LOCUS       DASRHN010000001         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_330072, whole genome shotgun sequence.
ACCESSION   DASRHN010000001 DASRHN010000000
VERSION     DASRHN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330072"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2328
                     /gene="mfd"
                     /locus_tag="VFB37_00005"
     CDS             <1..2328
                     /gene="mfd"
                     /locus_tag="VFB37_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZO20849.1"
                     /translation="KGRVLIAADSPGRREVLQEMLRGHGHDITPVSDWEGFANGRAAL
                     ALTVAPDLHGLTLTDPPIAVISEAQLFGARARQERRRKRAAADPEAILRDLQSLEPGA
                     PVVHEEYGVGRYVGLQIMEIAGQPGEFLILEYLDGDRVYVPVHSLHLVSRYTGAAPES
                     APLHKLGTDQWARARKRAAEQIRDVAAELLDLYARRKAQQGLKLPVNELEYQAFANAF
                     PFEETADQAEAIRQVLNDLQSDRPMDRIVCGDVGFGKTEVAMRAAFAAVQAGKQVAVL
                     APTTLLAQQHLTNFRDRFADWPVRVEALSRFGNSKQTQAVIEGLEHGSVDIVIATHRL
                     LHSHARFSDLGLLIIDEEHRFGVRDKERLQALRANVHVLTLTATPIPRTLNMALGGLR
                     DLSLITTAPAERLAIKTFVIEWHGPTLREAALRELRRGGQIYFVHNEVKTIEKIAAEV
                     RTLVPEASVRVGHGQMRERELEQLMVDFYHRRFDLLVCTTIIESGIDVPTANTMVINR
                     ADRIGLAQLHQLRGRVGRSHHRAYAYLVAPPRKALAGDAAKRLEAIESMEELGAGFVL
                     ATHDLEIRGAGELLGEQQSGQMTEIGLALYLDMLEQAVNALKLGREPALDRPLAAATE
                     VELRTPAFLPESYVADVHVRLGLYKRLAAADSPTELDELTAEIHDRFGPLPPVAQSLL
                     KVARLKLTARTLGIRRLDFGPQGGSVWFEEQHHLDPDVVVRLIQKHPREYRLEGSLKL
                     RVSRALASEQARFEFAAELMRRLSQKPARAGGKLQ"
     gene            2338..2949
                     /locus_tag="VFB37_00010"
     CDS             2338..2949
                     /locus_tag="VFB37_00010"
                     /inference="COORDINATES: protein motif:HMM:NF022425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HZO20850.1"
                     /translation="MKPLLASLLLSVLAIGLPALSQDSGPTYHIEVIVFRAASPQGGP
                     ENWSAEAGAANTVGDESASGSAQVGHFVALLPAAQYQLTDIETRLRSSGAFIPIAHFA
                     WAQTASAWGTRAGFPLQKLGVDVQGLSGNVALERGQYLHLGVSLSYVAASPPAGLGAA
                     PGTSFTLNESRRVRFYERNYYDNPAFGVIALVTPAQGSRPAGR"
     gene            complement(2990..3922)
                     /locus_tag="VFB37_00015"
     CDS             complement(2990..3922)
                     /locus_tag="VFB37_00015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO20851.1"
                     /translation="MNNWSAEAWRGVAETAPEGIVVCDAAAADHPVVYANAAFAQMCG
                     YPVSALLGTNLRILQGNDRDQEARLRIREALERGEPARVLLRNYRPDGTMFWNETVLQ
                     PVRNRTGRVTHTIGYHRDASDRLKSSERNSQGLPPWLREDRLTGLHSRAYFEELLQRD
                     WRLAQRDSHEIGLTLVDIDDLGPYNDTFERVGGDACIRRIARVIGASYRRGGDLVGRW
                     EGGTFAVLTQGDAAEKASQYAQVVAQRVRDLLIHHPRGAAERYVTISAGVASLVPPRE
                     LPLESLLKACNAALKRSKKLGKNSVSTAEATDFQ"
     gene            complement(3919..4974)
                     /gene="nagZ"
                     /locus_tag="VFB37_00020"
     CDS             complement(3919..4974)
                     /gene="nagZ"
                     /locus_tag="VFB37_00020"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZO20852.1"
                     /translation="MSLGPLMIDLEGKAVTSEEQQMLQHPLVGGVILFTRNFVDPEQL
                     TQLVASIHAVRSPALIVAVDHEGGRVQRFRSGFSLLPTARRIGHEFDLDPRAGLDLAR
                     DMGWLMAAELRSCGVDISFAPCVDLDYGISEVIGERAFHSRSEVVGQLANAYVHGMRE
                     AGMAATAKHFPAHGAVVADSHLSLPVDRRELADLAADLAPYRRLIADGLTAVMAAHVL
                     FPAVDPAPASLSARWIRDVLRGDLRFQGVVFSDDLSMGGAAAAAGNIVTRARQALAAG
                     CDMLPVCNDRASVTALLQGLDVEPEPASRLRLVRLHGREGLTRAELTSRAEWQRSREL
                     LARCSAAPMLHLEAGGS"
     gene            complement(4971..>5800)
                     /locus_tag="VFB37_00025"
     CDS             complement(4971..>5800)
                     /locus_tag="VFB37_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO20853.1"
                     /translation="PLAAMLNGLSIRERLPQIEIAVADNAVALVLRVLEPPSAADLVR
                     LREFAAAHSVRLYLQPRGLHSIHPLDAPADAPAPLEYRLSRFDLRLQFAPTDFVQING
                     PVNEALVARAVDLLGLESNSVVLDLYCGIGNFTLALARTAAKVVGVEGDASLVQRARD
                     NARLNALDNVEFHAADLGQALEPQTPWLHHRYTHVLLDPPRAGAAAVLPVIARLEPRG
                     LLYISCHPGSLARDAGLLVNQHGMSLLAAGVLDMFPHTTHFESMALLGPATRTQGAA"
BASE COUNT         1004 a         1953 c         1933 g          910 t
ORIGIN      
        1 aagggccggg tcctgatcgc cgccgactcc cctggccggc gcgaagtgtt gcaggagatg
       61 cttcgcgggc acggtcatga catcacgccg gtgagtgatt gggaaggctt cgccaacggc
      121 cgggctgcac tggccctgac cgttgccccc gaccttcatg ggctgacact gaccgatccg
      181 ccgattgccg tgatctccga agcccagctc ttcggcgccc gagcgcggca ggagcgccgc
      241 cgtaagcgtg cggcagcgga ccccgaggcc atcctgaggg atctgcagag cctggagccg
      301 ggtgcgcccg tcgtgcacga ggagtatggc gtcggccgct acgtggggct gcagatcatg
      361 gagatcgcag gtcaacccgg cgaattcctg atcctcgagt atctcgacgg ggatcgggtg
      421 tatgtgccgg tgcattcgct gcacctggtg agtcgctaca cgggggccgc ccccgagagc
      481 gcacccctgc acaagctcgg gaccgaccag tgggcgcgcg cgcgcaagcg cgccgcggag
      541 cagatccgcg atgtcgccgc cgagctgctc gatctgtacg cgcgccgcaa ggctcaacag
      601 gggctgaagc tgcccgtgaa cgagctcgag taccaggcgt tcgccaacgc cttcccgttc
      661 gaggagactg ccgatcaggc ggaggcgatc cgccaggtgc tcaacgatct gcagagcgat
      721 cgtccgatgg accgcatcgt ctgcggggac gtcggcttcg gcaagacgga ggtggcgatg
      781 cgggcagcct tcgccgccgt gcaggcgggc aagcaggtcg cggtgctggc gccgacgacc
      841 ctgctcgcgc agcagcatct gaccaacttc cgcgaccgct tcgccgactg gccggtgcga
      901 gtggaggcgc tgtcccgctt cggcaactcc aagcagaccc aggctgtgat cgagggactc
      961 gagcacggct cggtggacat cgtgattgcg acgcaccggc tgctgcactc ccatgcacgc
     1021 ttcagcgatc tgggactgct catcatcgat gaagagcatc gcttcggtgt ccgcgacaag
     1081 gagcgcctgc aggctttgcg cgccaacgta catgtgctga ctctcacggc cacgccgatt
     1141 ccgcgcaccc tcaacatggc cctgggcgga ctgcgcgatc tctcgctgat caccaccgcg
     1201 ccggctgagc gcctggcaat caagactttc gtcatcgaat ggcacggacc gacgctgcgt
     1261 gaggcggcgc tgcgcgagct gcgtcgcgga gggcagatct acttcgtgca caacgaagtc
     1321 aagacgatcg agaagatcgc tgccgaggta cgcacgctgg tgccggaggc gagcgtacgg
     1381 gtcggccacg ggcagatgcg cgagcgagag ctcgagcagc tgatggtcga cttctatcat
     1441 cgccgcttcg atctgctggt ctgcacgacc atcatcgaga gtggcatcga cgtgccgacc
     1501 gccaacacca tggtcatcaa tcgcgccgac cggataggtt tggcacagtt gcaccagctg
     1561 cgcgggcggg tcggccgctc ccatcatcgg gcctacgcct atttggtcgc cccgccgcgc
     1621 aaggcgctgg cgggcgatgc cgccaaacgc ctcgaggcga tcgagtcgat ggaggagctc
     1681 ggagccggct tcgtgctggc cacacacgat ctggagatcc gcggcgcggg cgagttgctc
     1741 ggcgagcagc agagcgggca gatgaccgag atcggcctcg ccctctacct ggacatgctc
     1801 gagcaggcgg tgaatgcgct caagctgggg cgtgagccgg ctctggatcg gccgcttgcg
     1861 gccgccacgg aggtcgagtt gcgcaccccg gcgtttctgc cggagagcta cgtcgcggac
     1921 gtgcacgtgc gtctcggact gtacaagcgc ctcgccgctg ccgactcacc gacagagctc
     1981 gatgagctca ccgccgagat tcatgatcgg ttcgggccgc tgccgcccgt ggcacagagc
     2041 ctgctgaaag tggcgaggct caagctgaca gcccgtacgc tcgggatccg gcgcctggac
     2101 ttcgggcctc agggaggcag cgtctggttc gaggagcagc atcatctcga tcccgacgtg
     2161 gtcgtgcggc tgatccagaa gcatccgcgc gaatatcgtc tggagggctc cctgaagctg
     2221 cgcgtctcgc gagccctcgc gagcgagcag gcccgcttcg agttcgccgc cgagctcatg
     2281 agacggctga gccagaagcc ggcgcgggct ggcggcaagc tacagtagaa tcagcgcatg
     2341 aagcctctgc tggcctccct gctgctgtcc gtactggcga tcggcttgcc cgcactgtcg
     2401 caggacagcg gcccgaccta tcacatcgag gtgatcgtct ttcgcgcagc ctccccccag
     2461 ggcggcccgg agaactggag tgcggaagcg ggcgccgcca acacggtcgg cgacgagtcc
     2521 gccagcggct ccgcgcaggt cggtcatttc gtggccctgc tgccggcggc gcagtaccaa
     2581 ctcaccgata tcgaaacccg cctgcgctcg agtggcgctt tcattccgat cgcacacttc
     2641 gcctgggccc agaccgcgag cgcgtggggc acaagggccg gatttccgct gcagaagctc
     2701 ggcgtcgatg tgcaggggct gtcgggaaac gtagcgctcg agcgcggcca gtatctacat
     2761 ctgggtgtct cgctgagtta tgtggcggcc tccccgccgg ccggcctcgg cgcagcaccc
     2821 ggaacctcat tcacgctcaa cgagagccgc cgcgtgcgct tttacgagcg caactactac
     2881 gacaatccgg ccttcggggt gatcgcactc gtgacgccgg cgcaggggag tcgtcctgcc
     2941 ggacggtagt ggcagcgcgc gagcctcgcg gcgggcgcaa gcggaacggc tactggaaat
     3001 ccgtggcttc ggctgttgag acgctgttct tgcccagctt cttcgagcgc ttgagcgcag
     3061 cattgcaggc cttcagcagc gactccagag gcagctctct gggcggcacg aggctcgcga
     3121 cgccggcact gatcgtgacg tagcgctcag cggctccgcg cggatgatgg atcagcagat
     3181 ctcgcacgcg ctgtgcaaca acctgagcgt actgggaagc cttctcggcc gcatcgccct
     3241 gcgtgagcac cgcgaaggtg ccaccttccc agcgaccgac cagatcgccc ccccgccggt
     3301 aggaagcacc gatcacgcgt gcgatccgtc ggatacaggc atcaccccca acgcgctcga
     3361 aggtgtcgtt gtacgggccc agatcgtcga tatcgacgag tgtcagcccg atctcatgcg
     3421 agtcgcgctg cgccaggcgc cagtcgcgct gtagcagctc ctcgaaataa gcccgggaat
     3481 gcaggcccgt caggcgatct tcacgcagcc acggcggcag cccttggctg ttgcgctccg
     3541 agctcttcag ccgatcgctc gcatcgcggt gatatccgat ggtgtgagtc actcgcccgg
     3601 tgcggttacg caccggctgc agcaccgtct cattccagaa catcgtgccg tcgggacggt
     3661 agttacgcag cagtacccga gcgggctccc ctcgctcgag cgcctcgcga atccgcaacc
     3721 gggcttcctg atctcggtcg ttaccctgga gaatgcgcag gttggtcccc agcagcgcgc
     3781 tgacgggata gccgcacatc tgggcaaagg ctgcattggc gtacaccacg ggatggtcgg
     3841 cggcggctgc atcgcagacc acgatgccct ccggcgcggt ctccgcgacc ccacgccagg
     3901 cttccgcgga ccaattgttc atgaaccgcc ggcctccaaa tgcagcatcg gcgcagccga
     3961 acagcgcgcc agcagctccc ggctgcgttg ccattccgcc cgggaagtca gctccgcgcg
     4021 ggtcaacccc tcgcgcccat gcaaccgaac caggcgcagc cgggaagccg gctcgggctc
     4081 cacatcgagc ccctggagca gtgctgtcac gctggcccgg tcattacaga ctggaagcat
     4141 gtcgcaaccg gcggccagcg cctggcgtgc acgggtcaca atattccccg ctgccgcagc
     4201 cgcgcctccc attgacaaat cgtcgctgaa cactaccccc tgaaagcgca aatctccgcg
     4261 caacacgtcg cgaatccagc gcgcggacag gctggccggt gccggatcga ccgccgggaa
     4321 caggacatgg gccgccatga cggccgtgag cccgtcggcg atcaggcgcc gatagggggc
     4381 caggtcggcc gccaggtctg ccagctcccg ccggtccacc ggcaggctca ggtgggagtc
     4441 cgctacgacg gccccatgcg ccgggaagtg cttggcggtg gcggccatcc ccgcctcccg
     4501 catgccgtgc acgtaggcgt tggcgagctg gcccacgacc tcggaccggg aatgaaacgc
     4561 gcgctccccg atgacctcgc tgatgccgta gtccaggtcc acacagggcg cgaatgaaat
     4621 atccactcca cacgagcgca gctccgcggc catcaaccat cccatgtcgc gtgcgagatc
     4681 gagccccgcg cgagggtcca gatcgaactc atggccgata cgccgggcag tcggcagcaa
     4741 cgagaagccc gatcggaagc gctgcacgcg gccaccctcg tggtccaccg cgacgatcag
     4801 cgccggggag cggacggcgt ggatgctcgc gacgagctgc gtgagctgct ccggatccac
     4861 aaagttgcgg gtgaagagaa tcacgcctcc gaccagagga tgctgcaaca tctgctgctc
     4921 ctcggaggtc acggctttgc cctcgagatc gatcatcagt ggccccaaac tcatgctgcc
     4981 ccctgcgtgc gcgtggccgg ccccagcagg gccatggatt cgaagtgagt cgtgtgcgga
     5041 aacatgtcga gtactccggc cgccagcagg ctcatcccgt gttgattcac cagcaagccc
     5101 gcatcccgcg ccaggctgcc gggatggcag gagatgtaga gcaggccgcg cggctcgagg
     5161 cgggcgatca ccggcagcac cgcggccgcg ccggcgcgtg gcggatcgag cagcacgtgg
     5221 gtatagcggt ggtgcaacca gggcgtttgc ggctcgaggg cctgaccgag atcggcggcg
     5281 tgaaattcca cgttatccag tgcgttcagg cgggcattgt cccgcgcccg ctgaacgagg
     5341 ctcgcatcgc cttccacgcc aacaactttc gcagcagtgc gagccagggc cagagtgaag
     5401 ttgccaatgc cgcagtacag atcgagcacc acggagttgg actccaggcc gagcagatcc
     5461 actgcgcgcg ccaccagcgc ctcgttgacg ggtccgttga tctggacgaa gtccgtcggc
     5521 gcgaattgaa gccgcaggtc gaagcgcgac agccggtact cgagcggcgc cggggcatcc
     5581 gcgggggcat cgagcggatg gatggagtgc agaccccgcg gctgcagata gaggcgcacg
     5641 gaatgtgcgg cggcgaattc gcgcaggcga acgagatcgg cagcgctcgg cggctccagg
     5701 actcgcagca cgagtgccac cgcattgtct gccacggcga tttcgatttg cggcaagcgc
     5761 tcgcgaatgc tcaggccatt cagcatcgcc gcgagcggcg
//
LOCUS       DASRHN010000002        19937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1154429, whole genome shotgun sequence.
ACCESSION   DASRHN010000002 DASRHN010000000
VERSION     DASRHN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19937
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154429"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFB37_00030"
     CDS             complement(<1..478)
                     /locus_tag="VFB37_00030"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HZO20854.1"
                     /translation="MWTRAVLSGVSRVWPGVTLLGLAPALLSQAASAPTPTAVSQAAS
                     MPERPAYTASGQLLLPASYREWIYLSSGFDMSYRPGRQAEHHEFDNVFVNPTAYKAFV
                     QTGRWPDGTLFVLEVRAAEGKGSINTSGNYQGAGVMELEMHVKDESRFPGKWAFFSF"
     gene            550..1281
                     /locus_tag="VFB37_00035"
     CDS             550..1281
                     /locus_tag="VFB37_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20855.1"
                     /translation="MSTDPRAPPDTAPYPRSLRYLATGLNTVTIILVITVLAISDTRP
                     WLLVVLALLPWIAIALVARFQPIYRFGARRGDPHPDLTLPLMVPGLVMVLPVLSSLHT
                     LDWKAPLAIAGVLGIALAGAAALVDPWFRQHRWNVLLVAVFACAYGYAVGMEINAHAD
                     PTDPRIYPVHVLTKHVSRGSKSTTWYLKTAPWGPYSQSQEVSVSSTRYRETQPGDLVC
                     VYMGRGAVGIAWYRLDDCSEGTRAF"
     gene            complement(1365..3167)
                     /locus_tag="VFB37_00040"
     CDS             complement(1365..3167)
                     /locus_tag="VFB37_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO20856.1"
                     /translation="MDELGTALGRFAGGLSNIDEMRNVFREYLRSHPEKREAVSHWVS
                     ESIRIGRISPAIMLTIGDLIGPLPQEATGPDTLSTVRPSVSPPGMTLASLVSAPATAS
                     TIAPQSMIPPPPAVGMPRATADPDATGARLPESGNAAAVAEEDATLALGHVLDGRYTL
                     MEELGRGGMGAVFKARDRNREAFQDRHPYIALKVLSEEFKRHPDARMALQRETVRAQN
                     LAHPNIVTVFDFDYDGPHAYMTMELLEGHSLEDWLQQESSATAPMARRWSIIRSIGAG
                     LAYAHEKGVVHSDLKPGNVFLCKSGTVKVMDFGIARPLRAVTDQTDTTLFDPAERLGG
                     LTPAYASMEQWNHEAPDPRDDIYAFACVVYYIFSGQHPFGRVSAKVACESRLTPQRIS
                     SLTRRQWDALRRGLAFKRNDRIPSVEQFLRQFAPLTWYQKYRKWLMGAAALAATVILI
                     LAAQYYRDYVADQSINAQLWPSTELAVPPPTAEQRRDIDDYLYLAQEALKQAANARSD
                     EERSAILSKGDNNLLDLLKSVRGLEPSNAQALQLTDDATHLYENRARALLEADQLPDA
                     LKLVLEGQKFEHTRVLFEIKRTICRRKAALCLGR"
     gene            complement(3302..3943)
                     /gene="tagF"
                     /locus_tag="VFB37_00045"
     CDS             complement(3302..3943)
                     /gene="tagF"
                     /locus_tag="VFB37_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HZO20857.1"
                     /translation="MSTSECWTERIGWFGKIPAAGDFVGRGLPANFVRSWDEWLSAGL
                     CEARHLLSEHWTEAYRQAPVLCLLLGPGVLTESAWRGILLPSMDRLGREFPLIVAQCS
                     APALVEASGHQGVEAPKQEWWDSLVRVAQQSLEHARSADAMEELLAMFVRGRAADLLT
                     DAPDCEPPAARILAESGQSAWWRFEAGRLPTEATTIFQGLPPGPELRRLLGFG"
     gene            complement(3940..7329)
                     /gene="tssM"
                     /locus_tag="VFB37_00050"
     CDS             complement(3940..7329)
                     /gene="tssM"
                     /locus_tag="VFB37_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HZO20858.1"
                     /translation="MLKSWLLALAGLTAFALLVWLAGPLLIVGGQAPLASPQVRAFVI
                     VVFVLQYVAQKLWSARQARRNNERVVAGLTPAAEPGDSSEAQVLRRRFSTALSELKSA
                     RFASRGAWRFGRSYLYELPWYAIIGSPGAGKTTALLNSGLNFPLTDKLGRGPVGGFGG
                     TRNCDWWFTDRAVLIDTAGRYTTHDSDRSADRAGWETFLNLLRQTRPRQPLNGVLVAV
                     SVGDLLEFTPAELLEHAQTLRSRIEELERSLRRRMPLYLLLTKCDLLPGFVDWFGALG
                     RKERDQVWGVTFDLDSEQPDSTARFPAKFERLVSHLTDGLVERMQAERDPQRRARIYS
                     LPGQLRGLEGPLDALVSRAFGPASATGGTSTFLRGVYLTSGTQAGTPIDRMLVAFGRE
                     LGLERQILPPNQNTGKSFFLAGLLNDVVLAEAGLTGGARTPRWRRRLLVSAIAAMQLS
                     AVALGAWWVTSYLRAVHDIAQLDEEVTRARSLVHSIPPGSDPDPRPVLPALDALRELA
                     NREGRPRGSVGFFDVGARARTKLIAAARQSYEQVLLGPFQSRVAGAIDTTLRNGADVN
                     LQYEALKAYRMLNDARHFDADGLRIFVLAYWDSALAPAPTLSERKALTSHLEAFLRAG
                     AVGSNIRLDPMLVDSVRDRLRSQSSAQRLALRLAVRLNSHHDPDFMLASLGPTAAALF
                     VGTDGSSPPKPVAGRYTIDAFRDVVSGQLQPMAKQLASESSWVLGSAQPDAGADASQV
                     LAAYQGEYGQAWAQFLDEVHLKAAASSAEAVSQAQALGRQDGPLELLLGAIVFQTPLR
                     LASGAEGPIAPSDPAADRFKALADLFTRAAGGGATIDGVMHSFTEVQILRTPGGTNTA
                     KPDAAERIARVVSAAKTDPEPVRSMLLSLAVLTPGNGAAPVPRASPGSLSRQIAARLE
                     VSCLRAVAGRFPFDRRAARDTSFEDFAQLFAPKGAFDEVFGQLLAAHVDTASDNWSWR
                     GPGGPGAEELERFRASARIREVFFPHGGSQPAFQLTFRPLDMDETIDRLQLEIDGQTV
                     RYAHGPPVATVVKWPGPQGSARIELTPAAAGSLEEYTGPWALFRLLDHAAVQEGSAPG
                     HFKVVFDVGGRHASFDVTTDDGANPFRLRELERFDCPIASR"
     gene            complement(7329..8438)
                     /locus_tag="VFB37_00055"
     CDS             complement(7329..8438)
                     /locus_tag="VFB37_00055"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HZO20859.1"
                     /translation="MAARLLFVAEPVLLLVCRLRAAATVADLSRLRERLSEVLLDFQA
                     SAQTQGIESRRVAQATEVLRVLIDHVVTSMPWGADAGWQSLAQTSAGAGRPAQRLLDM
                     ARAASSDSELAELISVAVSLGFERRSRGAEDPSVDQLLALLAAPGQKLNPHAKQSLSP
                     EGRSPVQRTAATGWLPLWTSTLIMAALLAALYFALQVSLGIKSDRIYARIAAMDIPQV
                     TSVGQATSAPRLQPALSEQIAQGNFSVRDETDRSVIVVPAAKLFQGDAATLQSSATGV
                     LQPIATVLQHTPGRIRVVGHTDGTVARSARFPSAWEFSVERARVVQEALRGLGIEASR
                     LSYDGRANIEPLAGAQRTGSAAADSRIEIVLLVGR"
     gene            complement(8438..8953)
                     /gene="tssJ"
                     /locus_tag="VFB37_00060"
     CDS             complement(8438..8953)
                     /gene="tssJ"
                     /locus_tag="VFB37_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HZO20860.1"
                     /translation="MLRRIGATVRAACIVASLLLAFGTGEQRALADPASSPSAATTLE
                     LTIVGGPALNPNTEGRPSPVVVRVFDLAASRNFEGADYQALFEQPNDLLKQDVVAQEE
                     LLLRPGDIQERSRTLSPQVHALGIAAAFRDLDRAVWRLTVPLKPGRRNFLLIDLDKNT
                     VRLDTGDSGRP"
     gene            complement(8956..10155)
                     /gene="tagH"
                     /locus_tag="VFB37_00065"
     CDS             complement(8956..10155)
                     /gene="tagH"
                     /locus_tag="VFB37_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HZO20861.1"
                     /translation="MTLVLRGVTLNEEPISRPLIGRFDERGGTLGRSDDATLTLPDPE
                     RMISRLQGQIVHSDSHYWIENISAASAILHNGRPLGIGMRVVLRHGDELRIGGYTLEA
                     AFEDDASNATILSGRTVVPRVDTVPAARRAAPPQPQVSLQPPASPQPPASPQPSAPSQ
                     PPTPLSPQPGAELHATLAEPLRGGLQATLQNAAGAEALWRAFLQGAGIEVPLAPSSEL
                     LTTIGEMMKIAVGGIQRLVTMRARAKSEMQADMTLIQSRDNNPLKFSPDPDQALQMLL
                     QPPARGFLAGPAALRDAVTDLQSHQVGMTAGMRSVLEAVLDRLDPAKIEGLPAKRSMS
                     DLFNPARRQARLWAIYLQQYQGLRDEAQDGFERIFGEVFREAYEAQVRNLGASGESDR
                     PRGGGSR"
     gene            complement(10297..12117)
                     /locus_tag="VFB37_00070"
     CDS             complement(10297..12117)
                     /locus_tag="VFB37_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO20862.1"
                     /translation="MDTSLSATEALKPGTDAPGARTDARHARADPRVAEVLYSTAVEG
                     DRRAVVITILGSFLVAFLVQRQTASHAAWLWCTACVTLGGLRLALNWPSRRSEVSDSA
                     ASARWRWLALLLVGFTVWGVGPLLFLMDNPSADTLLTGIFLAAAGLSAPLLSAWRPAV
                     YVSLLPSLLPLLVTLAMRPLTGPTPVSTNSLLLASIGAAFLLVLERLTMTQSDSLALL
                     LATRFRNEDLVSQLRSQIEVAARANQEKTRFLASAAHDLRQPLHALGMFCAALEQRLN
                     NTPEKPLVRNMMSAIESLEESFGAMLDISRLDAGVVQTSPQTFAIRDTFRRLYQQFAG
                     DAEARNLALRFRATRRIVRSDPLLLERVLANLVQNALRYTRKGGVLVAARRHASGVAL
                     EVWDTGLGIPADKREMIFREFYQIDNPERDRGRGLGMGLAIVQRLCSLLEHPLDLAST
                     PGRGSVFRLIVPVGEVSAIDAANTDADTLPPRRSGQVTVILIDDERTIREATRELLRP
                     MHVNVLLAGTIAEAVALARDSGEQIEMILSDWRLRGQENGVEAVRAVRAVCGEATPAV
                     LITGDTSPELLKLAHESGLVVLHKPLQPRQLVRLIKHLRR"
     gene            complement(12149..12778)
                     /locus_tag="VFB37_00075"
     CDS             complement(12149..12778)
                     /locus_tag="VFB37_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO20863.1"
                     /translation="MDTNKKVLLVDDHDLVRFGTSLLLESLEEVRCEIIPCRSLDDAR
                     TACREKGPFDLVLLDLNLPDAKGLQGLRTLLDEHPNMPFAMLTGTQDEFVIRQAQAMG
                     AVGYLLKSWTPERLKEALKSLLRPTAVPRSGTIAERFPKLAGSSHYDRVAELGPRHLQ
                     ILELILEGCSNQEIAKATELSLGTVKNYVSAILLALDVESRSHLISLFH"
     gene            complement(12853..13581)
                     /locus_tag="VFB37_00080"
     CDS             complement(12853..13581)
                     /locus_tag="VFB37_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZO20864.1"
                     /translation="MKTLVRLDRQAGATMVEFALVMPFALLLVLGLIQIGLMYSAKAV
                     VNEAAFMAARAGAVQNAQVDKMTAAMQKALIPFYQDTTNSDAATRLTAALASAKADTD
                     CASASQCFLKIEVLNPTPAAFADFGITSGASQGHTFIPNDNLEFRSHAVKGKTGGSGE
                     SIQDANALKIKVTYGYELKVPLIATLFKAVLCGTDSGVDAFGRANSPLSIQGDDDCTN
                     FYDQGRIPIVTYATVQMQTPAWQQ"
     gene            complement(13615..14169)
                     /locus_tag="VFB37_00085"
     CDS             complement(13615..14169)
                     /locus_tag="VFB37_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO20865.1"
                     /translation="MTSRIGSLLAGHARRAASLLLLASLAACATAPAPKQAAKEASQT
                     RPAGPAQSADSDQQSKKFQHAEALYLSGHLQEAAAAFEELTRAYPTDARIWFRYGNTE
                     AKLGAYDDAAKAFQTALAVEPGQGPAALNLALIRLAQAQESLDVAIAGLPAQSPEGKQ
                     AGGLKSQLDALVGTPLKGSAPPQH"
     gene            14393..14689
                     /locus_tag="VFB37_00090"
     CDS             14393..14689
                     /locus_tag="VFB37_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20866.1"
                     /translation="MNRITLSKRTAGARRQFGQGMTEYIIIVALIAIGAIAVYSAFGR
                     TVQSQMAAISNGLAGKGSVANGDVTQAGTEAGKADTDSKANLGLDSYTKQVTDQ"
     gene            14878..16359
                     /locus_tag="VFB37_00095"
     CDS             14878..16359
                     /locus_tag="VFB37_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZO20867.1"
                     /translation="MRSPIRGANRARQGGQTVVWFLATIAACCCVFALVYNVGQVTNK
                     KEATVNAADAAALSGALIEARMLNFEAYANRAMVANEVTVAQLVSSESFMYYTDTLLQ
                     NIATYTAAVPFVDDVTGPLSDGVDLVTTGVDAKIRVDIPIIEGVNTILQGVAAGVYAS
                     GATAANSVASTIAAANETTFNGRDDQAPQLAASTLALAMNESTWSSFISIEDQSILKD
                     IVNNSRDPFADQRNNSSLINDFNTALEILGAGTEFPQFAKTSGVTKLDDFNHWEAQDS
                     MDLEENTCFLGHFCSTDPVPFVDMGYGRADADSDGSTGTDQCNTSGGLFGQFTWNCQQ
                     AMNNANNLSWNGLPKLYDIVAGLGNKDPCTVNNGSDSGSMAYVMAVQEAGKATLTTQN
                     LGTGLNNVDVAGPQGSPRMTDNFQNGGDLTAISEACVFFLRPDGKAVDPTRGSLARED
                     GQHEFASLYNPYWQARLTAPDSKWITALYAQIGQASLIPVATQ"
     gene            16442..17209
                     /locus_tag="VFB37_00100"
     CDS             16442..17209
                     /locus_tag="VFB37_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20868.1"
                     /translation="MSEFVAAMALFIPLVLGIIYIGKFSDIRHQAIQASRYAAMERAL
                     DPRKSESDTIVQNETVARFFRDGGAHNIAKDEQAQGSTATDENPNWSQITGDPLIAQY
                     SDISVKLGQKGSLSTPADSAAGLFKGLGSGSGVEADVEVPVVNVTHFAPLANINLKIG
                     ATTVMAGDPWNGDGAADVASHQSDESVPGKTAPFQLLKDVLTPFVEALIDTPPPQFGC
                     VKPEVVPKTGGLGAPGAVYNPLHDPGDAGDPNDFCYN"
     gene            17220..17978
                     /locus_tag="VFB37_00105"
     CDS             17220..17978
                     /locus_tag="VFB37_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20869.1"
                     /translation="MKTTAPQLFRRTLELVTIGALLAVLTLPGTARAASEPWPEIPMP
                     PKADVQWIATSMRVNGIPTRIMRFQSHVSRAEIISYYRAYWTGAYEHKPSISALGDAT
                     VVGQTHGPYLMTVKVEDAPKSGSHGLISVALVIGSKLDHDPGQLPMLPGAHVISVVES
                     DDPGKHSRQLLILAPQAPTSALEFYSDSLLNSGWAQVQSTEARQGAGPAGGVFVAFAH
                     DDSEMQLSIVGTARGRGTTILANLVTKDTGRDGD"
     gene            18021..18245
                     /locus_tag="VFB37_00110"
     CDS             18021..18245
                     /locus_tag="VFB37_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZO20870.1"
                     /translation="MNRLRNTIGPMSHGRLARSGFIPGRERGQSMTEYAIIGGILAAL
                     LFAATSPVGQVLTTALHNFYQDLTFFLSLP"
     gene            18256..19059
                     /gene="cpaB"
                     /locus_tag="VFB37_00115"
     CDS             18256..19059
                     /gene="cpaB"
                     /locus_tag="VFB37_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZO20871.1"
                     /translation="MKFAPKLPSLNNNWLLFAGAIALGLVATFLFQKQLRDHKSQIDA
                     ELRGAHQMVDVVVAKRDLPKGAVISPAVFARDRIPAEYVHASAIDPKHFGQYAGQRLG
                     APLKRGEALLQVHLESLTAVFSSTLENGNRALTTEVDEVNSISGMLRPADHIDLMVTA
                     HGSGANATDVTFPLLSNVEVLATGQVTRKSEGTNQPRTYTTITLSVSPEDAQRIVVAK
                     NSGKLTAVLRNPDDARAAPTAAMNIDDVLPKKAAVGHHMAVQYIIGGRS"
     gene            19446..>19937
                     /locus_tag="VFB37_00120"
     CDS             19446..>19937
                     /locus_tag="VFB37_00120"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HZO20872.1"
                     /translation="MDDAHSCHIEPNENRHMKPNFLCNLLALGLVAQVALAAPQPEQP
                     ATADAAARAADSNTRSTDERHPARSVVNGPPLSLYHGEVQVLDLADVSRIAVGNGDVL
                     RANVVASNQVVLIGQAAGTTSLRVWTRNGSQFTYEVNVRAFDVAQILRDVQDLLAGEP
                     GISA"
BASE COUNT         3641 a         6563 c         6289 g         3444 t
ORIGIN      
        1 cgaacgagaa gaaggcccat ttgccgggaa aacggctctc atccttgacg tgcatctcca
       61 gttccatgac cccggctccc tggtaattcc cgctcgtatt gatcgagccc ttgccctccg
      121 ccgcgcgcac ctcgagcacg aacagggtcc cgtccggcca gcgacccgtc tgcacgaatg
      181 ctttgtaggc ggtcggattg acgaacacat tgtcgaactc gtgatgctcg gcctgccgtc
      241 ctggccgata gctcatgtcg aatcccgagc tcagatagat ccactcgcga tagcttgccg
      301 gcagcagaag ctggccgctg gctgtgtagg ccggcctttc gggcatgctc gcggcctggc
      361 tcaccgcagt cggcgtgggt gcgctcgccg cctggctcaa cagggccggc gcgagcccca
      421 gcagcgtgac gcccggccac acccgcgaga cgcctgaaag cacagctcga gtccacatgc
      481 ccggaagcgt accccacgcg aaggagggaa ggcgggatat cattgcgccg ggcggttcac
      541 agcagcgaca tgagtacaga tcctcgagcg ccgcccgaca cggcgcccta tccgcggagc
      601 ctgagatatc tcgccaccgg tctcaacacg gtgacgatca tcctcgtcat taccgttctg
      661 gccatctcgg atacccgccc ctggctgctg gtcgttctgg ccctgctgcc ctggatagcg
      721 atcgcgctcg tggcccgctt tcagcccatc taccgcttcg gtgcccggcg cggcgatccg
      781 caccccgacc tgacactgcc tctgatggtg cccgggctgg tcatggtgct gcccgtgctg
      841 tccagccttc acaccctgga ctggaaggca ccgctcgcga ttgccggcgt gctcggaatc
      901 gcgctggcgg gcgcagcggc ccttgtcgat ccctggttcc gtcagcaccg ctggaacgtg
      961 ctgctggtcg ctgtattcgc gtgcgcctac ggctacgcgg tcggcatgga gatcaatgcg
     1021 catgcggatc ccaccgatcc gcgaatctac cccgttcatg tcctgaccaa gcacgtcagc
     1081 cgagggtcga aatccaccac ctggtacctg aagactgcgc cgtggggccc gtattcgcag
     1141 agtcaggaag tcagtgtcag ttccacgcgt taccgggaga cccagccagg ggacctcgtg
     1201 tgtgtctaca tgggacgggg cgccgtcgga attgcctggt atcgccttga tgattgcagc
     1261 gagggtactc gcgcattttg agcgggccag cccacatttc gcagcgggcc aacgcacctc
     1321 ctgtgccgta atgcgcttgc ctccgaggaa tcgtgcgctc gcggtcagcg acccaggcac
     1381 agcgcggctt tgcgacgaca gatcgtacgc ttgatctcga aaagcacccg cgtgtgctcg
     1441 aatttctgcc cctcgagaac gagcttgagc gcatccggga gctgatcggc ctcgagcagc
     1501 gcgcgcgccc ggttctcata gagatgagtg gcatcatcgg tcaactgcag cgcctgggcg
     1561 ttggaaggct ccagcccgcg cacgcttttc agcaggtcga gcaggttgtt gtcgcccttc
     1621 gacagaatcg ccgagcgctc ctcgtcgctg cgcgcattcg ccgcctgttt cagtgcttcc
     1681 tgcgccaagt acaggtaatc atcgatgtcg cgtcgctgct cggccgtcgg gggcggcacg
     1741 gccagctccg tgctaggcca gagctgcgcg ttgatggatt gatcggcgac ataatcgcgg
     1801 tagtactgcg ccgccagtat caggatgacc gtcgccgcca gagccgctgc ccccatcagc
     1861 cacttccggt acttctgata ccaggtcagg ggcgcaaact ggcgcaggaa ctgctcgacg
     1921 gagggaatcc gatcattgcg cttgaacgca agcccacgcc gcaaagcgtc ccactgtcgc
     1981 cgagtgagtg aggagattcg ctgcggagtc aggcgggact cgcaggcaac cttcgccgag
     2041 acccggccga aaggatgttg gccgctgaag atgtaataga cgacgcaggc gaacgcatag
     2101 atgtcatccc gcggatccgg agcctcgtgg ttccattgct ccatggaggc gtaggccgga
     2161 gtcagtccgc cgagccgctc cgcgggatcg aataaagtcg tatccgtctg atcggtcacc
     2221 gcgcgcagtg gccgggcgat cccgaaatcc atgaccttga ccgtgccgga cttgcacaga
     2281 aacacgttgc cgggcttcag gtcggagtgg acgacaccct tctcgtgcgc gtaggccaga
     2341 cctgctccga tgctgcggat gatggaccag cggcgcgcca tgggggcggt ggcgctcgat
     2401 tcctgctgca accaatcctc gagcgagtga ccctccagca gttccatcgt catgtaggca
     2461 tgcgggccgt catagtcgaa atcgaacacc gtgacgatgt tgggatgcgc caggttctgg
     2521 gcgcgcaccg tctcgcgctg cagcgccatg cgggcatcgg ggtggcgctt gaactcctcg
     2581 ctcagcactt tcagcgcgat gtaaggatgg cggtcctgga aggcttcccg attgcggtcc
     2641 ctagccttga acaccgcccc cataccgccg cgtcccagtt cctccatgag cgtgtagcgg
     2701 ccatcgagca catggccaag ggcgagtgtg gcgtcctcct ctgcaaccgc agcagcattg
     2761 ccactctcgg gtagccgggc acccgttgca tccgggtctg ccgttgcacg gggcatcccg
     2821 accgcgggcg gcggagggat catgctttga ggggcgatcg tggaagcggt cgccggcgct
     2881 gagaccaggg aggccagtgt catccccggt ggtgacacac tcgggcgaac cgtggacagg
     2941 gtatccggcc cggtcgcctc ctgcggtagc gggccgatca ggtcgccgat cgtgagcatg
     3001 atggccgggg agatgcgacc gatccggatg ctctcgctca cccagtgcga gacggcttca
     3061 cgcttctccg gatgagagcg caggtactcg cgaaagacgt tgcgcatctc atcgatattg
     3121 ctcaggccgc ccgcaaaccg gcccagcgcc gtgcccagct cgtccatctt gtcccctgta
     3181 tcacctgaca ggtgaggctt ccgggaagcg ttcccgcagc ccatcggccc attctcgcgc
     3241 accgggcagt gaagttcgac tgtcctgggt cgaccgggtt ggcggttttt cgcccgctta
     3301 ttcagccaaa gcccagcagc ctgcggagct cgggtcctgg gggcagtccc tgaaagatcg
     3361 tcgtggcctc ggtcggcaac cttccggcct cgaaacgcca ccaggcactc tggccggatt
     3421 cggccaggat ccgcgcagct ggcggctcgc aatccggagc gtcggtcagc aggtctgcag
     3481 ctcgtccgcg aacaaacatc gcgagcaact cctccatggc atcggcgctg cgagcatgct
     3541 ccagagactg ctgtgccaca cggacgagcg aatcccacca ctcttgtttc ggagcctcca
     3601 ccccctgatg ccccgaagcc tccacaagcg caggtgcact acactgcgcg acgatgagcg
     3661 gaaactcgcg cccgagccgg tccatgctgg gcaggagtat tcctcgccag gcgctctccg
     3721 tgagcacgcc gggaccgagc agcaaacaca gcaccggcgc ctgccgatac gcctctgtcc
     3781 agtgctcgct cagcaggtgg cgggcctcgc agagccctgc cgacagccac tcgtcccagg
     3841 acctgacgaa attcgcaggc aaaccccgcc cgacgaaatc ccccgccgcg ggaatcttcc
     3901 cgaaccagcc gatcctctcc gtccagcact ccgaggtgct catcggctgg caatcggaca
     3961 gtcgaagcgc tccagctcgc gcagtcgaaa tggattggcg ccgtcgtcgg ttgtgacatc
     4021 gaaactggcg tgccggccac cgacatcgaa cacgactttg aaatgaccgg gcgcgctgcc
     4081 ttcctgaacg gccgcgtgat cgagcagtct gaacagcgcc cagggcccgg tgtattcctc
     4141 gagcgatccg gcagctgcgg gagtcagctc gatgcgcgca ctgccctgcg gcccaggcca
     4201 cttcacgacc gtggccaccg gcggcccatg ggcgtaccga accgtctgcc cgtcgatctc
     4261 gagttgcagg cgatcgatgg tctcatccat atccagcggt cgaaaggtga gctggaacgc
     4321 tggttgggag ccgccatgtg gaaagaacac ctcacgaatg cgagccgagg cgcggaagcg
     4381 ctccagctct tcggcacccg ggccgcccgg gccacgccac gaccaattgt ccgaggccgt
     4441 atcgacgtgt gccgcgagca actggccgaa cacttcgtcg aaggctccct tgggcgcaaa
     4501 cagctgcgcg aagtcttcga aggaggtatc gcgcgcggcg cggcgatcga agggaaatcg
     4561 tccggccacc gctcggaggc agcttacttc gagccgggcc gcgatctgac gcgacagcga
     4621 gcctggcgag gcgcgcggca ccggagcagc tccattacct ggagtgagca ccgcaagtga
     4681 cagcagcatc gagcgaaccg gctccgggtc cgtcttcgcc gcgctgacca cccgcgcgat
     4741 tcgctcagcg gcatccggct tcgccgtgtt tgtccctcca ggggtgcgca gtatctgcac
     4801 ctccgtgaag gagtgcatca cgccatcgat cgtcgcgccg ccacccgcgg cacgcgtgaa
     4861 gaggtcggcc agggccttga agcgatccgc tgcgggatcg gacggtgcaa tggggccctc
     4921 ggctccgctc gcgagccgca atggtgtctg aaagacgatc gcacctaaca gcagctcgag
     4981 gggaccgtcc tgtcggccga gtgcctgcgc ctggctcacc gcctcggcac tcgaggcagc
     5041 cgccttcaga tgcacctcgt cgaggaactg agcccaggcc tggccatact cgccttgata
     5101 ggcggccagc acctgagacg catcggcacc ggcatccggc tgcgcgcttc cgagcaccca
     5161 gctcgactcg gaggccagct gcttcgccat cggctgcagt tgtccgctca ctacatcgcg
     5221 gaacgcatcg atcgtgtagc gacccgcgac aggcttgggg ggagagcttc catcggtgcc
     5281 gacaaacagt gcagccgcgg tcggccccag agaggccagc atgaaatccg gatcgtggtg
     5341 cgagttgagg cgcaccgcca atctcaacgc gaggcgttgc gcgctggatt gcgatcgcaa
     5401 gcgatcgcgc accgaatcca ccagcatcgg atcgaggcgg atgttcgacc ctactgcacc
     5461 agctcgcaga aaagcctcca ggtggctcgt caaggccttg cgctcggaga gagtcggtgc
     5521 aggagcgagc gccgagtccc agtaggcgag aacgaagatc cggagcccgt cggcatcgaa
     5581 atgtctggcg tcgttcagca ttcgataggc tttgagcgcc tcatattgca ggttgacgtc
     5641 cgccccgttc ctcagcgtcg tgtcgatcgc gcctgcgacc cggctctgaa aaggtcccag
     5701 cagcacctgc tcgtaacttt gccgcgcagc ggcgatcagt ttcgtgcggg cacgcgctcc
     5761 gacatcgaaa aatccgacgc tcccgcgcgg acggccctca cggttggcca gctcccgcag
     5821 agcatcgagt gctggcagaa caggcctggg atcgggatcg cttcccgggg gtatcgagtg
     5881 aacgagggag cgggctctgg tgacctcctc gtccagctgt gcaatgtcgt gaacggcgcg
     5941 caggtagctt gtaacccacc acgcgccgag cgccacggcg ctcaactgca tcgcggcgat
     6001 ggctgacacg agcagccgac gccgccagcg cggggtccgt gcgccgccgg tgaggcctgc
     6061 ctccgccagc actacatcgt tgagcagccc cgccagaaag aagctcttgc cggtgttctg
     6121 attgggcggc aggatctgtc gttccagacc gagttcgcgg ccgaacgcca ccagcatccg
     6181 gtcgatcggt gttccggcct gtgtgccgct cgtcagatac acgccccgca gaaatgttga
     6241 tgtcccaccg gtggcgctcg cgggcccgaa cgcacgactt acgagcgcat cgagaggccc
     6301 ctccaaaccg cgaagttggc ctggcagcga gtagattctg gcgcgccgct gcggatcgcg
     6361 ttccgcctgc atgcgctcga ccaggccgtc ggtgagatgg ctcaccagac gctcgaattt
     6421 ggcggggaag cgcgctgtgc tgtcaggttg ctctgagtcg aggtcgaacg tgacgcccca
     6481 gacctggtca cgctccttgc gcccgagcgc tccgaaccag tcgacgaagc cgggcagcag
     6541 atcgcatttc gtcagcagca ggtacagtgg catgcgcctg cgcagggaac gctcgagctc
     6601 ctcgatgcgg ctccgcagcg tctgcgcgtg ctcgagcaac tcggcggggg tgaactcgag
     6661 gaggtcgccg accgagacgg cgaccagcac tccgttcaaa ggctggcggg gtcgtgtctg
     6721 tctcaggagg ttgaggaagg tttcccagcc ggcccgatcg gcgctccgat cgctgtcgtg
     6781 cgtggtatag cgcccggcgg tgtcgatgag cacggcgcgg tccgtgaacc accagtcgca
     6841 gttgcgcgtg ccgccgaacc cgcccaccgg gccccgtccc agcttgtccg tcagggggaa
     6901 attcaaaccg gaattgagca gcgccgtggt ctttcccgcc ccgggtgaac cgatgatcgc
     6961 gtaccagggc aattcataca gatacgaacg gccgaaccgc caggcaccgc gcgaggcgaa
     7021 gcgcgcgctt ttcaactcgg acagcgcggt ggagaagcgt cggcgcagca cctgcgcttc
     7081 ggacgagtca ccgggctcag ccgccggagt cagccccgcc acgacgcgtt cgttgttccg
     7141 tcttgcctga cgggcactcc agagcttttg cgccacgtac tgcagcacga agaccacgat
     7201 gacgaacgcg cgaacctgcg gtgaagcaag cggggcctgc ccgcccacaa tgagcaacgg
     7261 accggcgagc cacaccagca gcgcgaatgc ggtgaggcca gcgagcgcca ggagccatga
     7321 cttgagcatc agcgccccac cagcagcacg atctcgattc ggctgtctgc ggcagcggag
     7381 ccggtccgct gcgcgcccgc gagcggctcg atgttggctc gaccgtcgta gctgagtcgc
     7441 gaggcctcga tgcccaaccc tcgcaaggct tcctgaacca cgcgcgcccg ctcgaccgag
     7501 aattcccacg cagatggaaa acgcgccgag cgcgccaccg tcccatcggt atggccgacc
     7561 acccggatgc gccctggcgt gtgctgcaat accgtggcaa tgggttggag caccccggta
     7621 gcgctggatt gcagggttgc cgcatcgccc tgaaagagct tcgcggcggg cacgacgatg
     7681 acgctgcgat ccgtctcgtc gcgcacgctg aaattgccct gcgcaatctg ctccgaaagc
     7741 gctggctgca ggcgcggagc cgaagtcgcc tgcccgactg aagtgacttg cggaatatcc
     7801 atcgcggcga tgcgcgcgta gatgcggtcc gacttgattc cgagtgagac ctggagcgcg
     7861 aagtagagcg ccgcgagcaa ggccgccatg atcaaagtgg aggtccagag tgggagccaa
     7921 ccggttgccg ccgttcgttg caccggcgag cggccttccg gcgaaagact ctgttttgcg
     7981 tgcggattga gcttctggcc cggcgctgcc aacagggcga gaagttgatc gacgctgggg
     8041 tcctccgctc cgcggctgcg tcgttcgaat ccgagcgaga cggccacgct gatcaactct
     8101 gcgagctccg aatccgacga agcggcacgc gccatgtcga gcaggcgctg agcgggccgg
     8161 cctgcgcccg cggacgtctg ggccagcgac tgccagcccg catccgcacc ccagggcatg
     8221 gaggtcacca cgtgatcaat caacacgcgg agcacttcgg ttgcctgcgc tacgcgacgg
     8281 gattcgatgc cctgcgtctg tgcgctcgcc tggaaatcca acagcacttc ggacaggcgc
     8341 tcgcgcaggc gcgagagatc cgcgacggtg gcggcggcgc gaagccggca caccagaagc
     8401 aggaccggct ctgcgacgaa gagcaggcgg gcagccatca gggcctaccc gaatcgcccg
     8461 tatccaggcg gaccgtattt ttgtcgagat cgatgagcag aaagtttcgc cggccgggtt
     8521 tcagggggac cgtgagacgc cagacggcgc ggtcgaggtc gcgaaaggct gcagcgatgc
     8581 ccagtgcgtg aacctgtggc gataaagtgc ggctgcgctc ctggatgtcc ccgggtcgca
     8641 gcaacagctc ctcttgcgcc accacatcct gcttcagcag atcattgggc tgctcgaaca
     8701 aggcctgata gtcggcgccc tcgaagttgc gactggccgc caggtcgaag acccgcacga
     8761 cgacaggcga cgggcgccct tcagtgttgg gattgagcgc ggggccgcca acgattgtca
     8821 gctcgagcgt cgtggcggct gaaggactgg aagcagggtc ggccagcgct cgttgttccc
     8881 ctgtgccaaa cgcaagaagc aggctggcca cgatgcaggc cgcgcgcacc gtcgcgccga
     8941 tgcgtcgcaa catcctcacc gggagcctcc gccgcggggc cggtcggact ccccgctcgc
     9001 gcccagattt cgcacctgcg cttcgtaggc ctcgcggaac acttccccga agatccgctc
     9061 gaagccgtcc tgcgcctcat cacgcagacc ctggtactgc tgcagataaa tcgcccacag
     9121 gcgggcctgg cgccgggccg gattgaaaag gtccgacatc gagcgcttgg caggcaggcc
     9181 ctcgattttc gcgggatcga gccgatcgag gacagcctcc agtacagatc gcatcccagc
     9241 agtcatgcca acctggtggg actgcaggtc cgtcacggca tcgcgcaggg cggcgggccc
     9301 tgcgaggaag cctcgcgccg gcggctgcag cagcatctgc agagcctggt cggggtcggg
     9361 cgagaacttc agcgggttgt tgtcgcgcga ctggatcaga gtcatgtcgg cctgcatctc
     9421 gctcttggcc cgcgcgcgca tcgtgaccag ccgttggatg ccgcccaccg cgatcttcat
     9481 catttcgccg atcgtggtca gaagctcgct cgagggcgcc aacggcacct cgataccggc
     9541 tccttgcaga aaggctcgcc agagcgcctc ggcacccgcg gcattctgaa gagtcgcctg
     9601 caatccgccc cgcaggggct cggcaagcgt cgcgtgcagt tcggcgcccg gctggggact
     9661 gagaggcgtc gggggctgag aaggcgcgga gggctgcgga gatgccggcg gctgagggga
     9721 cgctggaggc tgaagagaca cctggggctg aggaggtgcg gcccggcgtg cagcggggac
     9781 ggtgtctact cgaggaacga ccgtccgtcc gctgagaatc gttgcgttgg aggcatcgtc
     9841 ctcgaaggct gcctcgagcg tgtagccgcc gatcctcagc tcgtcgccat gacgcaggac
     9901 cacccgcatg ccgatgccca ggggtcggcc gttgtgcaga atcgcgctgg ccgcgctgat
     9961 gttctcgatc caatagtgac tgtcgctgtg gacgatctgc ccctgcaggc gggagatcat
    10021 gcgctccggg tccggcagag taagcgtcgc atcatccgag cggcccagcg tgccgccgcg
    10081 ctcgtcgaac cgcccgatca gcgggcggct gatcggctct tcattcagcg tcacacctcg
    10141 caagacaagg gtcatacgtc attcccctcc gacagcccat ttcccgcgca tggacggcgc
    10201 gctccgtgcc cgtcagcctg gccgggacgg ctcggccgcg caaatcacta aagtcatatt
    10261 gacgggcatg cccctcgcgg ccccccggac gggctgttag cgccgcaagt gcttgattag
    10321 acggacgagc tgccgaggct ggaggggctt gtgcagcacc accagcccgc tctcatgtgc
    10381 cagcttcagc agctcgggcg aggtgtctcc cgtgatcagg accgccggcg tcgcctcccc
    10441 gcatacggcg cgcactgccc gcaccgcctc gaccccgttc tcctggccgc gcaaccgcca
    10501 gtccgacagg atcatctcga tctgctcacc cgagtcgcgc gcgagggcaa ccgcctcagc
    10561 gatggtgccg gccagaagca cattcacatg catcggccga agcagctcgc gggtggcctc
    10621 ccggatcgtg cgttcgtcat caatcaggat caccgtgact tggcccgacc tgcgcggggg
    10681 caacgtatcc gcatcggtgt tcgccgcatc gatcgcgctg acctcgccca ctggaacgat
    10741 caggcgaaag acactccccc tgccgggggt cgaggccaga tcgagcggat gctcgagcag
    10801 gctgcacaga cgctgcacga tggccaatcc cattccgagg ccccggccgc gatcgcgctc
    10861 gggattatcg atctggtaga actcgcggaa gatcatttcc cgcttgtcag cggggatgcc
    10921 gaggccggta tcccacacct cgagtgccac cccgctcgcg tgccgcctcg cagcgacgag
    10981 cacgccgccc ttgcgcgtat agcgaagcgc attctggacg agattggcca gcacacgctc
    11041 gaggagcagt gggtcgctgc ggacgatccg gcgcgtggcg cgaaatcgca atgccagatt
    11101 gcgcgcctcc gcatcacctg cgaactgttg atagagccga cggaaggtgt cgcggatcgc
    11161 gaacgtctgc ggagaagtct gcaccacgcc cgcatccagg cgcgagatgt cgagcatcgc
    11221 cccgaacgac tcttcgagcg actcgatggc gctcatcatg ttgcgaacca gaggtttctc
    11281 cggcgtattg ttgagccgct gctcgagcgc tgcacagaac attcccagag catggagcgg
    11341 ctgccgcaga tcgtgagcgg cggaggccag aaagcgcgtc ttctcctggt tggcgcgcgc
    11401 cgcgacctcg atctgcgagc gcagctggct caccagatcc tcgttgcgga agcgcgtagc
    11461 gagcagcagt gccagagaat cgctctgcgt catcgtcaga cgctcgagga ccagcagaaa
    11521 tgcagcgccg atggatgcca gcaggagtga gttggtcgag accggcgtcg gtcccgtgag
    11581 cgggcgcatc gcgagcgtaa ccagcagtgg caggagagag ggcaacaatg aaacatacac
    11641 agctggccgc cacgcggaga gaagtggagc tgacagtccc gcagcagcca ggaaaatacc
    11701 ggtcagcagc gtgtcggctg acggattgtc catcaggaac aacagcggtc ccacgcccca
    11761 gaccgtgaat ccgaccaaca gcagggccag ccagcgccag cgggcgctgg cggcggagtc
    11821 gctcacttcg ctccgacgcg acggccagtt cagagccagc cgcaggccgc cgagcgtcac
    11881 gcacgccgtg caccatagcc atgctgcgtg cgatgccgtt tgtcgctgca ccagaaaggc
    11941 aaccaggaac gagcccagga tcgtgatgac gaccgcccgg cgatcgccct cgaccgcggt
    12001 gctgtagagg acttcggcga ctcggggatc cgcacgcgcg tggcgcgcat cagtccgcgc
    12061 cccgggcgca tcggttccgg gtttcagggc ctcagtggct gacaggctgg tgtccacccg
    12121 catgaagaga tggacctggt cgcagggttt agtggaacag gctgatcagg tgcgatcgcg
    12181 actcgacatc gagcgccagc aggatagccg acacgtagtt cttcaccgtt ccgagtgaca
    12241 gctcggttgc cttggcgatc tcctgattgc tgcagccctc cagaatcaac tcgaggatct
    12301 gcagatggcg gggacccagc tcggcaacgc gatcgtagtg cgaggagccg gcgagtttgg
    12361 ggaaacgctc ggcgattgtc cccgacctgg ggactgcggt cggccgtaac agtgacttga
    12421 gcgcctcctt caggcgctcg ggcgtccagg acttgagcag atagcccacc gcgcccatgg
    12481 cctgcgcctg gcggatgacg aattcatcct gcgtcccggt gagcatcgcg aacggcatgt
    12541 tgggatgttc atcgaggagc gtccgcagcc cctgcaggcc cttcgcatcg gggagattga
    12601 gatcgagcag cacgagatcg aacggtcctt tctcgcggca ggcagtgcgc gcatcgtcca
    12661 gtgagcggca aggaatgatt tcgcaccgca cctcttccag cgactccagc aggaggctgg
    12721 tcccgaagcg caccagatcg tggtcatcga ccaggaggac cttcttgttc gtgtccatca
    12781 gcactcccca tgtccgcagc ggaggctagc gccgccgcgg cgagcttgtc cagttttcgc
    12841 cccggctgcc tctcattgct gccaggccgg ggtctgcatc tgcaccgttg cataggtgac
    12901 gattggaatg cgtccctgat cgtagaagtt cgtgcagtcg tcatcgcctt gaatgctgag
    12961 tggagagttc gcgcggccga aggcatccac gccactgtct gtcccgcaca gaaccgcctt
    13021 gaagagcgtg gcaatcagag gcaccttgag ctcgtacccg taggtcacct tgatcttcag
    13081 cgcattggca tcctggatcg actcaccgga gcctccggtc ttgcccttga ccgcgtggga
    13141 gcggaactcc agattgtcgt ttggaatgaa cgtgtgtcct tgcgaggcgc cgctcgtgat
    13201 gccgaagtcc gcgaaggccg ccggcgtagg gttgagcacc tcgattttga gaaagcactg
    13261 actcgccgat gcgcagtcgg tatccgcttt cgcactcgcc agcgccgctg tcagccgcgt
    13321 tgcggcatcc gaattcgtgg tgtcctgata gaacggtatc agcgcttttt gcatggccgc
    13381 ggtcattttg tcgacctgtg cgttctgtac cgcgcccgcg cgtgccgcca tgaaggccgc
    13441 ctcgttcact actgccttgg cactgtacat cagaccaatc tggatcagcc cgagtaccag
    13501 caggagcgcg aagggcatga cgagtgcgaa ctcgaccatg gtcgcgcctg cttgcctatc
    13561 gagccggaca agagtcttca tgatcactcc ttgcggattg cgtgaggacg cagatcagtg
    13621 ttgcggcggg gcggagccct tcaggggcgt tcccaccagc gcgtcgagtt gacttttcag
    13681 accgccagcc tgcttgcctt ccggtgattg ggccggcagc ccggcgatcg ccacatcgag
    13741 agactcttgc gcctgcgcca ggcggatcag cgccagattg agagcggcgg gtccctgccc
    13801 gggctcgacc gccagcgccg tttgaaatgc cttggccgca tcatcgtaag cgccgagctt
    13861 cgcctcggtg ttgccatagc gaaaccagat ccgggcgtcc gtcgggtagg cgcgcgtgag
    13921 ttcctcgaat gccgcggccg cctcctgcag atgtccgctg agataaagag cctcggcgtg
    13981 ttgaaatttc ttgctctgtt gatcggagtc cgcggactga gcgggcccgg cggggcgagt
    14041 ttgagatgct tccttcgcag cttgctttgg cgcaggtgca gtggcgcaag cggccaacga
    14101 tgccaggagc agcagcgacg cggcgcggcg cgcatgacca gccaacaacg aacctatacg
    14161 cgatgtcatc tgcctgactc ctcttcacta ctcgcccgcc tcgcggcggg tactaacaga
    14221 tcagtgaccc gaagagtact gcgagaaggg ccggccgtcc tgcaaaaacg ataggactaa
    14281 agtcaatagg caggcgtgcc ggatatgcgc accatgtgct ccgacgattt gaaccgcgcg
    14341 gcgccgactg gtcgcgcaac acacaaccca cacattcttg gaggatccga tcatgaaccg
    14401 cattactctc agcaagcgca ccgccggtgc ccgccggcag tttggccagg ggatgaccga
    14461 gtacatcatc atcgttgccc tgatcgccat cggcgccatc gccgtataca gcgcattcgg
    14521 ccgcaccgtg cagtcacaga tggctgcgat ttccaacggt ctggcaggca agggcagcgt
    14581 ggccaacgga gatgtcacgc aggccggcac cgaggctggc aaggccgaca cggattcgaa
    14641 ggcgaatctg ggcctcgaca gctacacgaa gcaggtcacc gatcagtaac agcactcgag
    14701 ccttcgttca ccggcgcggc ggccacgggg ccgccgcttt cgtttcggca cacgcccatc
    14761 ggatgcagac gcaatcccgc cgcggaacac gacattgact ttggtaatta ggcagcgccc
    14821 gcgtaaacga gcaggatcga tcacaacagc aagcctgccg atgcaaagga gcatgacatg
    14881 agatcaccca ttcgcggcgc aaaccgggcg cgccaaggcg gccagaccgt cgtctggttc
    14941 ctggcgacga tcgcggcatg ctgctgcgtg ttcgcgctgg tctacaacgt cggccaggtg
    15001 accaacaaga aggaagcgac cgtgaatgcc gcggatgccg cggcgctgag cggcgcgctg
    15061 atcgaggcca ggatgctcaa cttcgaggct tacgccaatc gcgcgatggt tgccaacgaa
    15121 gtgactgtcg cgcagctggt gagctccgag tccttcatgt actacacgga cacgctgctg
    15181 cagaacattg cgacgtacac ggccgcggtt ccgttcgtgg atgacgttac cggacctctc
    15241 tcggacgggg tggatctggt tacgaccggg gtggatgcca agatccgggt cgatataccg
    15301 atcattgagg gcgtgaacac gatcctgcag ggcgttgctg ccggggtcta tgcctccgga
    15361 gcgactgcag cgaactcagt ggcctcgacc atcgccgcgg caaacgagac gaccttcaac
    15421 gggcgcgacg accaggcgcc tcagcttgcg gcaagcaccc tcgctcttgc gatgaacgaa
    15481 agcacctggt cctccttcat cagtatcgag gaccagtcga tcctcaagga catcgtcaac
    15541 aactcgcgcg atccgttcgc ggatcagcgc aacaacagca gcctcatcaa tgacttcaac
    15601 accgcgctgg aaatcctggg cgccggcacc gagtttccgc agttcgccaa gacctccggc
    15661 gtgacgaagc tcgatgactt caatcactgg gaagcgcagg actcgatgga tctcgaggag
    15721 aatacctgct tcttgggcca cttctgctcc actgacccgg ttcctttcgt ggacatgggt
    15781 tatggccggg cggatgcgga ttcggatggg agtaccggca ccgatcaatg caacaccagc
    15841 ggcggactgt tcggccagtt tacctggaac tgccagcagg ccatgaacaa tgccaataac
    15901 ctgagctgga acgggctccc caagctttac gacatcgttg ccgggctcgg caacaaagac
    15961 ccttgcaccg tgaacaacgg cagcgatagt ggctcgatgg cctatgtgat ggcggtccag
    16021 gaggccggca aggcaactct gaccacccag aacctgggga ctggcctcaa caatgtggac
    16081 gtcgcgggtc ctcagggctc gccgcgcatg acggacaatt ttcagaatgg cggcgatctg
    16141 accgccatct ccgaggcgtg cgttttcttc ctgcgcccgg atggcaaggc cgtcgatccc
    16201 actcggggct ctctcgcccg tgaggacggt cagcacgagt tcgccagcct ctataaccct
    16261 tactggcagg cgcgcctgac ggcgcccgac tccaaatgga ttaccgcgct gtatgctcag
    16321 atcggccagg ccagcctgat tccggtcgcg acccagtgag ctgcacgccc gtccgaacat
    16381 tctgaccagg agcccgatca tgcgcaccct ttcgatgaga acgacccagc gaggccaggc
    16441 catgagcgag ttcgtcgcgg ccatggccct gttcattccc ctggtgctgg ggatcatcta
    16501 catcggcaag ttctcggata tcaggcacca ggcgatccag gcgagccgct acgcggcgat
    16561 ggaacgcgca ctcgatcccc gcaagagcga gagtgacacg atcgtgcaga acgagaccgt
    16621 ggcacgcttc ttccgtgacg gtggcgctca caacatagcc aaggacgagc aggcgcaggg
    16681 ctccacggcc acggatgaga acccgaactg gagtcagatc acgggcgatc cgctgatcgc
    16741 ccagtactcc gatatctcgg tcaagctggg tcaaaaaggc agcctgtcga cgccggcgga
    16801 ctcggcggcc ggcctcttca aagggcttgg ctcaggatcc ggagtcgaag ccgatgtcga
    16861 ggtgccggtc gtgaatgtga cgcacttcgc cccgctcgcc aacatcaacc tgaagattgg
    16921 agcgacgacc gtgatggctg gcgatccgtg gaatggagac ggcgctgcgg atgtcgctag
    16981 ccatcagagc gacgaatcag tgccgggcaa gaccgccccc ttccagctgc tcaaagacgt
    17041 gctgacaccg tttgtagaag cgctgatcga caccccaccg ccacagtttg gctgtgtgaa
    17101 gccggaggtg gtgcccaaga ccggcggcct cggcgcgccc ggcgctgtat acaacccgct
    17161 tcacgatccg ggcgatgccg gtgacccgaa tgacttttgc tacaactgag actgtcatca
    17221 tgaagaccac agcaccacag ttgtttcgac gtaccctcga gctcgtcacg atcggagcgc
    17281 tgcttgccgt tctgacactg ccggggacgg cccgagccgc ctcggagccc tggcccgaaa
    17341 tccccatgcc cccgaaagcg gacgtgcagt ggatcgctac gagcatgcgg gtgaatggaa
    17401 tccccacgcg catcatgcgg ttccagtctc atgtgagccg ggcggagatc atcagctatt
    17461 accgggcgta ctggaccggc gcttacgagc acaagccctc aatcagcgcg ctcggggacg
    17521 ctaccgtagt gggccagacg catggacctt acctcatgac ggtgaaggtc gaggatgccc
    17581 ccaaatcagg cagtcacggg ctgatctccg tggcgctcgt gattggatcg aagctggatc
    17641 acgaccctgg tcagttgccg atgctaccgg gtgctcacgt catctcggtg gtggagtccg
    17701 acgacccggg caagcacagc cggcagcttc tgatccttgc gccgcaggcg cccacttcgg
    17761 cgctcgaatt ttattccgac tcgcttctga attcgggctg ggctcaggtt cagagcaccg
    17821 aggcccgaca gggtgcgggc ccggcgggcg gcgtcttcgt ggcttttgcc cacgatgaca
    17881 gcgagatgca gctgagcatc gtcgggacag cccggggccg cgggaccacg atcctggcca
    17941 acctggtgac caaggacact gggcgggacg gcgattgatc ttcaaagtct caccgaagtg
    18001 cccgacacca ggaaactgcc atgaatcgcc tccgcaacac gattggtcca atgagccacg
    18061 ggagactcgc ccgtagcggc ttcatccctg gccgcgagcg tgggcagtcg atgaccgagt
    18121 acgcaatcat tggcggcatc ctggccgcgc tgctgttcgc cgcgacgtct cccgtcggac
    18181 aggtgcttac gacggccctc cacaatttct accaggatct gacctttttc ctttccctgc
    18241 cttgaggaca cacacatgaa attcgcaccc aagcttccga gcctgaacaa caactggctg
    18301 ttgttcgccg gggcgatcgc cctcggtctg gtcgcaacgt ttctgttcca gaagcagttg
    18361 cgcgaccaca agagccagat cgatgccgag ctgcgcggcg cccaccagat ggtcgatgtg
    18421 gtggtcgcca aacgggacct gccgaagggg gccgtgatct cccctgccgt gttcgccagg
    18481 gaccggatcc cggccgaata cgtccacgct tcggcgatcg atcccaagca cttcgggcaa
    18541 tatgccgggc agaggctcgg ggccccgctg aagcgcggcg aagccttgct tcaggtgcat
    18601 ctggagtctc tcaccgccgt gttcagctcc actctggaga acggcaaccg cgcgctcacc
    18661 acggaggtgg acgaggtcaa ttccatttcc gggatgctgc ggcctgccga tcacatcgat
    18721 ctgatggtca ccgcacacgg cagcggcgcc aatgcgaccg atgtcacctt tccattgctc
    18781 tcgaatgtgg aagtgctggc aaccggtcag gtgacgcgca agagcgaagg cacgaatcag
    18841 ccccgcacct acaccacgat cacgctctcc gtgagtcccg aggatgcgca acggatcgtc
    18901 gtagccaaga actcgggcaa gctcaccgcc gtcttgcgca acccggacga cgcccgcgcc
    18961 gcgcccactg ctgcgatgaa catcgacgac gtgctaccga aaaaggccgc tgtcgggcac
    19021 cacatggccg tgcagtacat catcggagga cgttcctagg atctcatcca ccgtttcatg
    19081 tatcacctcg caacgacgcc ggccttcctc catgggtcgc cggcgtctcc ctttgaccct
    19141 cgaatgtcct ggcccggcgt ttcctggaaa gatcccgcgt tctgattccc gcgttctgat
    19201 tcccgccgag ctgccgcgcc gattcctgcc gttctcgatc tcctgccgac ttcttagatc
    19261 gaccgctgcc ctttctcctt ccgatccctg ccctttctcg tcccggtccg cgccgccgcc
    19321 acgaccccct ctcatgagcc tcgcctgcgc cagccatatg cctttggtca tatcggccgg
    19381 ccgaagcctc ctcgacatgc cgttggtcac tatttggaga gcgtgcgcgc aggcaggatc
    19441 acgccatgga cgacgcgcac tcctgccaca tcgaaccgaa cgagaaccgt cacatgaaac
    19501 cgaacttcct gtgtaacttg ctggccctcg gcctcgtggc ccaggtagca cttgcggccc
    19561 cgcagccgga gcagccggca acagctgatg ccgctgcccg ggcggccgac tccaatacgc
    19621 gaagcaccga tgagcgccat ccggccaggt cggtcgtcaa tgggccgccg ctctcgctgt
    19681 accacggtga ggtgcaggtg ctcgatctgg ccgacgtgtc gcgcattgcg gttggcaacg
    19741 gtgacgtgct ccgcgccaac gtcgtagctt ccaaccaggt agttctgatc ggtcaggccg
    19801 ccggcacgac ttcgctgcgc gtgtggactc gcaacgggtc acagttcacc tacgaagtga
    19861 acgttcgtgc cttcgacgtc gcgcagatcc tgcgcgatgt gcaggatcta ctggctggtg
    19921 agcccgggat ctccgcc
//
LOCUS       DASRHN010000003         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2308823, whole genome shotgun sequence.
ACCESSION   DASRHN010000003 DASRHN010000000
VERSION     DASRHN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308823"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1331)
                     /locus_tag="VFB37_00125"
     CDS             complement(150..1331)
                     /locus_tag="VFB37_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HZO20873.1"
                     /translation="MPELRWTLLIIGVVFIAVLAWWERRRPHQASRDSLNDTVDSHPG
                     GKSEVGWGAEPERRGPPRVMREPTLTLPEIRVQGPASAPQELPVVEIAPEDSLAGLDV
                     GGDPVPVMRTGRDRPHEPDELENSADLEDLEESDRVQRLEESSKADPLEESDEVDRLE
                     ESGEADPLEGSDEIDPLEESDEISDETAEELEDPREREELRPHLERTGNVPPRGSVPR
                     PSPKPVEPASEAANTPQSAAPSSGEAALTRPEPIVDWPPEDARRLLTLRLVAPQHGRF
                     AGRAVRQALTAEGFVLGKFGIFHKPDAQNRAVVSAASLNKPGTFDLESIDTQRFGGLN
                     LFVVLPGPKTPLKAFDELVATARTLNERLEGALQDERGGPLTPMRAAALREALGAETA
                     S"
     gene            complement(1340..>2158)
                     /locus_tag="VFB37_00130"
     CDS             complement(1340..>2158)
                     /locus_tag="VFB37_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO20874.1"
                     /translation="ERLVNAAREAMEGARLAAQETRVRREAMAEQFAETRFELVEVQS
                     SLTAEASVPNWEHSLAQVRGDLERLGQVNLAAIDELRENSERKEYLDRQFQDLISALE
                     TLEHAMRRIDKETRTRFEETFERINVGMKEKFPRLFGGGHAYLELVGEDVLTAGVAVM
                     ARPPGKKNSTIHQLSGGEKALTAVALVFSIFDLNPAPFCLLDEVDAPLDEHNVGRFCD
                     IVREMSSRVQFIFITHNKTTMELASQLIGVTMNEPGVSRLVAVDVDEAMRLAAV"
BASE COUNT          368 a          741 c          655 g          394 t
ORIGIN      
        1 cgggtattcc gcttccagac cccgcagctc gaccatcagt ttgtcgtact cgccatccgg
       61 aatctccggg tcatcgagca cgtagtagcg tcggtcgtgg taagcgatct gcgcacgcag
      121 ctccgcggtc cgcgcctcgg cgtcgcgtct cacgaagcag tctcggcgcc cagagcttcg
      181 cgaagtgctg ccgctcgcat cggtgtcaaa ggaccgccac gctcatcctg cagcgcacct
      241 tccagacgct cattgagggt tcgcgccgta gcaaccagct catcgaaggc cttcagcggc
      301 gtcttggggc caggcagtac cacgaacagg ttgaggccgc cgaatctctg cgtatcgatg
      361 ctctcgagat cgaacgtgcc gggcttgttc aggctggcgg cactgacgac tgcacgattc
      421 tgggcatcgg gtttgtggaa gatcccaaac ttgccgagca cgaagccttc ggcggtgagt
      481 gcctggcgca cggcgcgacc ggcaaaccgg ccatgttgag gggctaccag acgcagggtc
      541 agcagacggc gggcatcctc aggaggccag tctacgatcg gctcgggccg cgtgagcgca
      601 gcctcaccgc tcgatggcgc ggcggactgc ggagtgttcg cagcttcgga cgcaggctcc
      661 acaggttttg gcgacgggcg ggggacggac ccccgaggag gcacattccc agtccgctcc
      721 agatgcggcc gcagttcctc cctctcacgc ggatcctcca gctcctctgc tgtttcgtca
      781 gagatctcgt cggattcctc gagcggatct atctcatcgg acccctccag cggatcggcc
      841 tcgccggact cttccagccg gtcgacctca tcggactcct ccagtgggtc cgccttgctg
      901 gactcttcca gccgctgcac ccggtcggac tcctcgagat cctcgaggtc cgcggagttc
      961 tcgagctcat cgggctcgtg cggccgatcg cgaccggtac gcatgaccgg gacgggatca
     1021 ccccctacat cgagcccggc cagcgaatcc tcgggggcga tttcgacgac cggcaactcc
     1081 tgcggcgcac tggccggtcc ctgaacccgg atctcgggca aggtcaacgt gggctcacgc
     1141 attacgcgag gcggccccct gcgttccggc tccgctcccc agccgacttc cgacttaccg
     1201 ccgggatgcg aatcaacggt gtcgttgagt gagtcgcgcg aggcttgatg ggggcgtcgc
     1261 cgctcccacc aggcaagcac ggcgatgaac accaccccga tgatcaggag cgtccagcgc
     1321 agctcgggca tgacgggccc tacaccgctg cgagccgcat ggcctcatcg acgtcgacgg
     1381 ccaccaggcg cgatacgcca ggctcgttca tggtgacgcc gatgagctga gaggcaagct
     1441 ccatcgtggt cttgttatgt gtaatgaaga tgaactgcac gcgtgaggac atctcccgca
     1501 cgatatcgca gaaacgaccc acgttgtgct catccagcgg cgcatcgacc tcatcgagca
     1561 gacagaaggg cgccggattc aggtcgaaga tcgaaaacac cagtgcgacg gccgtcaggg
     1621 ccttctcgcc gccggaaagc tgatggatgg tgctgttctt ctttccgggc gggcgggcca
     1681 tcaccgccac gcccgcggtc agcacatcct cgcccacgag ctcgagatag gcatgaccac
     1741 cgccgaacag acgcggaaac ttctccttca tgcccacatt gatccgctcg aatgtctctt
     1801 cgaagcgggt gcgcgtctcc ttgtcgatcc gccgcatggc gtgctcgagg gtttcgagcg
     1861 ccgaaatgag atcctggaac tgacgatcga gatactcctt gcgctcactg ttctcccgca
     1921 gctcatcgat ggcggcgaga ttgacctgtc cgagccgctc gagatcgccc ctcacctgcg
     1981 ccagcgaatg ctcccagttc gggacgctgg cctcggccgt caggctgctt tgcacctcca
     2041 ccagctcgaa gcgagtctcc gcaaactgct ccgccatggc ttcgcggcgc acgcgcgtct
     2101 cctgtgctgc gagccgtgct ccctccatcg cctcgcgagc ggcattgacc agcctttc
//
LOCUS       DASRHN010000004         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_990629, whole genome shotgun sequence.
ACCESSION   DASRHN010000004 DASRHN010000000
VERSION     DASRHN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990629"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..790)
                     /locus_tag="VFB37_00135"
     CDS             complement(119..790)
                     /locus_tag="VFB37_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZO20875.1"
                     /translation="MTLHWLSSGVRLSALITATALAAWMSAPASAQQQTLEGNPARGK
                     ALSYTCLGCHGVDGYKNAYPNYSVPELEGQHPEYLMAALHGYRDGDRAHITMHSQAST
                     LSDQDIADIASYFAGKPLAAGAKAIGTVPQAAQVCTSCHGQDGVGITPQYPSLAGQHP
                     DYIVRALEEYKKGGRKNPIMKGFASQLKDADMEVIADYFSQQRPSLATESRPYTTLGE
                     HSAAR"
     gene            936..1394
                     /gene="dtd"
                     /locus_tag="VFB37_00140"
     CDS             936..1394
                     /gene="dtd"
                     /locus_tag="VFB37_00140"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZO20876.1"
                     /translation="MIALIQHVSEAAVRVEEETIGAIGPGILALIGVRPADDLASAER
                     LLERVLGYRIFADELGRMNLSLRDTQGGLLLVPQFTLAADTHKGMRAGFSTAAPPEHA
                     RKLFEALLARARAVHSPVAGGSFGAHMRISLVNDGPVTFWLESAAEARPG"
     gene            1481..1729
                     /gene="tatA"
                     /locus_tag="VFB37_00145"
     CDS             1481..1729
                     /gene="tatA"
                     /locus_tag="VFB37_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZO20877.1"
                     /translation="MGIGMRELVVILLVVLLVFGAKKLRNIGEDLGAAVRGFKKSMNE
                     GEDEERTPPRQIRSDAPDAEFADSAKPREPAQKSDRPV"
     gene            1745..2191
                     /gene="tatB"
                     /locus_tag="VFB37_00150"
     CDS             1745..2191
                     /gene="tatB"
                     /locus_tag="VFB37_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZO20878.1"
                     /translation="MLDVGFSEILVIFVLALIVLGPEKLPKVAAQVGRWIGRARGMAR
                     QFREQLEEEVNLEQARKLQQAEPPPPITQRPSTPPPEPPKSYPDTYSHAHPTDAEGRP
                     VPAASSPPEAPAADPRQQDWVGGVSGDPSAQSAADAARAQSHERGT"
BASE COUNT          400 a          739 c          774 g          401 t
ORIGIN      
        1 ggcatggctt tcgcaagaag gccaatcagg atgtcctgca ggaaacggtg gcgacgttcc
       61 tgcagaagct ggcgcgttag ccagcgaatc ggaacgaacg agccgcgggc gagcggcttc
      121 agcgcgccgc tgagtgctcg ccgagcgtcg tgtaggggcg gctctcggtc gcgagggacg
      181 gccgctgctg gctgaagtag tccgcgatca cttccatgtc cgcatcctta agctgggagg
      241 cgaatccctt catgatggga ttcttgcggc cgcccttctt gtactcctcg agcgcacgga
      301 cgatatagtc ggggtgctgg ccggcgagcg aagggtactg tggagtgatg cctaccccgt
      361 cctgcccatg gcaggaggta cacacctggg ctgcctgcgg aaccgtccca atcgccttgg
      421 caccagcggc cagcggcttg cccgcgaagt aggaggcgat gtccgcgatg tcctgatcgc
      481 tgagggtcga agcctgggag tgcatcgtga tgtgggcgcg gtccccatcc cgatagccat
      541 gcagggcggc catgaggtac tcgggatgct ggccttcgag ctccggtacg ctgtagttcg
      601 gataggcgtt cttgtaaccg tccacgccat gacaacccag acacgtgtat gaaagcgcct
      661 tgcctcgagc aggattgccc tcgagtgttt gctgctgtgc gctcgccgga gcactcatcc
      721 acgccgcgag agctgtcgcg gtgatcagcg cgctaagacg gacccccgac gaaagccagt
      781 gcagcgtcat cgactactcc gcaacctgaa gcaaaaacgg ccccgaatct tacggactcg
      841 cccatgcaat tgccacattc agacccgatg gctgctgcca gtcggaccgg tccgcggccc
      901 gatcccgtgg agctgcttcc cgcggggcag gcggcatgat tgccctgatt caacatgtca
      961 gcgaagcggc ggtgcgggtc gaagaggaaa cgatcggcgc cattggtccg ggaatcctcg
     1021 cgttgattgg cgtgcggccc gcggatgatc tggcatcggc ggagcgactg ctcgagcggg
     1081 tgctcggcta ccggatcttt gccgatgagc tgggccggat gaatctgtcg ctgcgcgata
     1141 cccagggcgg gcttctgctg gtgccccagt tcacgctggc tgcggacacg cacaagggga
     1201 tgcgggcagg cttcagcacg gcagcgccgc ccgagcatgc ccgcaagctc tttgaggccc
     1261 tgctggcgcg ggcgcgggcc gtgcacagcc ccgtggccgg gggcagtttt ggtgcacaca
     1321 tgcgcatatc gctggtaaat gacggccccg tcacattctg gctggagagc gcggcggagg
     1381 cgcggccagg gtagccaaaa cggcgaactt tctcggtccg gcgcgctctt ttgccctatg
     1441 atcgatgggc tttccgcgca actagcagga gttcaagctt atgggtatcg ggatgcgcga
     1501 gctggtggtg atcctgctcg tcgtgctctt ggtgttcggc gccaagaagc tgcgcaacat
     1561 cggagaggat ctcggcgccg cggtgcgcgg cttcaagaaa tccatgaacg agggcgagga
     1621 cgaggaaagg acacccccgc ggcagatccg ctcggatgcc ccggacgccg agttcgccga
     1681 ttcggcgaag ccgcgcgagc ccgctcagaa gagcgatcgg ccagtctgag ctctcagtgc
     1741 cggcatgctg gacgtcggct tttccgagat tctcgtcatt ttcgtcctgg ccctcatcgt
     1801 gctgggcccg gagaagctgc cgaaggtcgc cgcacaggtg ggccgttgga tcggccgggc
     1861 ccgcggcatg gcgcgccagt tccgcgagca gctcgaagag gaggtcaacc tggagcaggc
     1921 tcgcaagctg cagcaggcag agccgccgcc gcccattaca cagcgcccct ccacgccacc
     1981 ccctgaaccg cccaagtctt atcccgacac ctactcccac gcgcacccca cggatgctga
     2041 gggccgtccg gtgcctgccg ccagctcacc gcccgaggct ccagccgccg acccccgtca
     2101 gcaggactgg gtgggcgggg tgagcggaga tccctcagcg cagagcgccg cggatgcggc
     2161 acgggctcag agccatgagc gaggaacctg aaaagctcgc cgaaggcacg ctgatctcgc
     2221 atctgctgga actgcgcgat cggctcatcc gcgcgctggc ggcggtcgcg ctggtattca
     2281 tcccttgcgc gctcaattcc aaccagatct tcac
//
LOCUS       DASRHN010000005         7085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_660972, whole genome shotgun sequence.
ACCESSION   DASRHN010000005 DASRHN010000000
VERSION     DASRHN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660972"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="VFB37_00155"
     CDS             complement(<1..1507)
                     /locus_tag="VFB37_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20879.1"
                     /translation="MSRALLAAWALVGLGSLTSSPAQQPATPAPSALPVPPEQVVSHA
                     SQTVDWYRHIVSLEQLPTDPEDVITRDRLYQTALTTVELAFDFGRAAAVLAAAPTSQG
                     QSGASGSKAGSGQNTVSGQNAVPGQNAASGQNAPSGQNAAGANSTTAGNTGGESASLD
                     RAAARLTARVAALQAQLNDLDQQIAQAPRKERDTLTAQRGEVSAALALTREIQDTVTQ
                     IERFEESSDTSGGVGASGLAAQIRDLQRSVPEARRPSGAHAAAVLAPAASGSGTESSS
                     SKTNSPPAASSAPTSNAASSATAAAAAFRPESAGVIALTGEWFSLASTRRQLNATAKQ
                     TDALLKELESLRTTVTHDVRTIAQQNLAVTSSDAAQIAQAREELDSAAMRFRQLSTLL
                     IPLGEQAISLESARNALSEWRNSLQSRSASVARYLLLRISVLLGSVVIVLIISDVWRR
                     ATFRYLHDSRRRMQFLALRRVVVSVALALVIIFGLVSEVGSVATYAGLITAG"
     gene            1539..3047
                     /locus_tag="VFB37_00160"
     CDS             1539..3047
                     /locus_tag="VFB37_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZO20880.1"
                     /translation="MVNLDEPAQSNPAQAPAPQRQEAAAAALRSVLPEDCVLWREAER
                     RPYECDGLTAFRQIPMLVALPRTEEQVHQVLRLCNRLDLPVVARGAGTGLSGGAMPHA
                     SGVLLSLARLNRILEINPLARQARVQPGVRNLQVSEAAASYGLYYAPDPSSQIACSIG
                     GNVAENSGGVHCLKYGLTIHNVLRLRGFTMEGELLELGSSALDGPGLDLLATVIGSEG
                     LLMIVTEVTLKLIPRPQLAQLVMASFDDVGKAGDAVAAIISAGIIPAGLEMMDRKATQ
                     AVEPFVRAGYDLSAEAILLAEADGSPEEVLEQIAEIERVLRAAGATALTVSHSEAERL
                     RFWSGRKNAFPAAGRVSPDYYCMDGTIPRKNLGHMLKAIEQMEAEHGLRCMNVFHAGD
                     GNLHPLILFDANQPGEWERADRFGAQILELCVQLGGTITGEHGVGVEKINSMCVQFSP
                     RERAAFFGIKRAFDPSGLLNPGKAIPTLARCAEYGKMHVHAGALPHADLPRF"
     gene            3052..4122
                     /gene="glcE"
                     /locus_tag="VFB37_00165"
     CDS             3052..4122
                     /gene="glcE"
                     /locus_tag="VFB37_00165"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HZO20881.1"
                     /translation="MEAELLRLREQVLIARRDRTPLRLRGAGTKDFYSASNSQSTPCT
                     TVDLRPFSGILDYEPSELVITARCGTPLSQIEAALAERGQFLGFEPPGFGADPTLGGI
                     VAAGLSGPRRLSAGAVRDFVLGAALLAGDGELLQFGGRVMKNVAGFDVARLLCGSLGI
                     FGIITQVCIKVLPRPRAEATVQLEMKAGEAIEAFNRWGSRPLPVSGAAWYDGGARIRF
                     SGAVPAVEKACTQIGGKRLDAAAAQRWWDSLRHYTHPLFSSDSLWRLSLPQTAAPLDL
                     SGDPLIDWGGALRWYAGDQGERDVRELAQRAAGTAMRWRGPAATGRFHPLSPAVAEIH
                     RRLKRAFDPEGIFNPGRLLADL"
     gene            4122..5396
                     /gene="glcF"
                     /locus_tag="VFB37_00170"
     CDS             4122..5396
                     /gene="glcF"
                     /locus_tag="VFB37_00170"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HZO20882.1"
                     /translation="MQTRLASEFRDRPEGRTADEILRACVHCGFCLATCPTYQLLGDE
                     LDSPRGRIYQIKQVLEGAPPSRAIQVHLDRCLTCRACETACPSGVEYGHLVDIGRAEV
                     DARTKRPLAERLYRWALREGVSRPRLFGFALRTGRAVSGLLPKRLRSKLGHRRPTGSW
                     PTQIHARKVLLPTACTQPSLSPPIDFATARVLDAVGLQSIVVQGAGCCGAIRQHLSDQ
                     EGALAQCRANIDAWWPYVESEQVEAIVVNASGCAAMIKEYGHLLRNDPQYSSKAAHIV
                     QRLRDPVQVLAPELERLRKRLSPQGSRRIAFHPPCTLQHALQIRGKVEGLLRALGAEL
                     VPVADAHLCCGSAGTYSLLQSDLAEELRTRKLETLLREQPDLVLSANIGCIAHLQGAC
                     AVPVRHWLEWVDAALTAGRTPPMSQTGKKEVR"
     gene            5403..6401
                     /locus_tag="VFB37_00175"
     CDS             5403..6401
                     /locus_tag="VFB37_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZO20883.1"
                     /translation="MRGNQTEFAFLTMPRYSMIALSNAVEPLRMANIQLGRPAYAWSI
                     TSLDGQPTPASNGLQLTPTVPLESIRSVDILFVCGGVDVQQGVSPRLLAALRRFADRR
                     IPLGALCTGGYALARAGLLDKYRATIHWENLSALREEFPRILLNDQLFTIDRDRFTCS
                     GGVAPLDLMLHLVKAKHGARISQLISEQFIVDRVRNDRDRQYVPLRAQIGVSHDTLIR
                     VAQLMEQNIEKPLSLDEIAAATELSRRQIERLFKRHLGCVPKRYYLQMRLRRARELLL
                     QTSMPIIDITTACGFQSPPHFSRCYRAQFGCPPSAERQIRHTKAGSGLRTRSDVLS"
     gene            6528..>7085
                     /locus_tag="VFB37_00180"
     CDS             6528..>7085
                     /locus_tag="VFB37_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HZO20884.1"
                     /translation="MAEQAQAEFNLRDLDDQELIEQVHDDLYNGLKSEVEEATHIFLE
                     RGWGAEKVLNEALVEGMRIVGIDFRDGILFVPEVLLAANSMKGGMEILRPLLAETGVE
                     PIGKIVIGTVKGDIHDIGKNLVSMMLEGAGFEVIDLGINNPVEKYLAALEEHKPDILG
                     MSALLTTTMPYMKVVIDRLKEQGIRK"
BASE COUNT         1203 a         2352 c         2306 g         1224 t
ORIGIN      
        1 gccccgccgt gatgagcccg gcgtacgtgg cgaccgagcc gacttcggag accagaccga
       61 aaatgatcac cagcgccagc gcaacgctga cgaccacacg tcgaagcgcg aggaactgca
      121 tccgccggcg tgaatcatgc aagtagcgga aggttgcacg ccgccagaca tcggagatga
      181 tcagcacgat gacgacggag ccgagcagca cgctgatgcg cagcagcagg taacgggcta
      241 ccgaggctga acgggactgc agcgaattcc gccattcgga gagcgcattg cgcgcactct
      301 ccagcgagat cgcctgctca cccaagggga tcagcagcgt cgagagctgc cggaagcgca
      361 tcgccgcgga atccaactcc tcgcgcgcct gcgctatctg tgccgcatcg gaggaagtca
      421 ctgccagatt ctgctgggcg atcgtgcgca catcgtgggt gaccgtcgta cgcaacgact
      481 cgagctcctt cagcagcgcg tcggtctgct tcgccgtcgc gttaagctgg cgccgtgtgc
      541 tggccagaga gaaccactcc ccggtgaggg cgatcacccc ggcggactcc gggcggaatg
      601 ccgcagcggc cgcggtcgcg gagctggctg cattgctcgt cggggcgctg ctggcagccg
      661 gcggtgagtt cgttttgctg ctcgagctct ccgttccact ccccgaagcc gcgggggcca
      721 aaacggcagc agcgtgagcg ccactcggac gccgcgcctc gggaaccgac cgctgcaggt
      781 cgcggatctg agccgcgagc ccgctcgcgc cgacgccgcc ggacgtatcc gagctttcct
      841 cgaagcgctc gatctgcgtc acggtgtcct ggatctcgcg tgtcagcgcg agcgcggctg
      901 acacttcgcc gcgttgtgca gtcagggtgt cccgttcctt acgcggggcc tgtgcgatct
      961 gctggtccag atcgttgagc tgggcttgca gcgcagcgac gcgggccgtc agacgcgctg
     1021 cagctcggtc cagggaagca gactcgccgc cggtgttgcc ggctgtggtg gaattcgcgc
     1081 cggccgcatt ctgaccagag ggggcattct gaccggaggc cgcattctgg ccggggacgg
     1141 cattctgccc ggagaccgta ttctgaccgg agccggcctt cgaaccggag gcgccactct
     1201 ggccctggct ggtcggtgcc gcggccagca ctgccgccgc ccgcccgaaa tcgaacgcaa
     1261 gctctactgt ggtgagcgcc gtttgataga gccggtcgcg cgtgatgaca tcttctggat
     1321 ccgtgggcag ctgctcgagg ctgacgatgt gtcgatacca gtccaccgtc tggctcgcat
     1381 ggctgacgac ctgctccggc ggcaccggca aggccgaagg cgcaggcgta gccggctgct
     1441 gcgccggact cgatgtgagc gagcccagtc caacgagagc ccacgctgcc aaaagagccc
     1501 tgctcatgtg gttctcatat tcgggttagc atgggccaat ggtcaacctg gacgagcccg
     1561 ctcaaagcaa cccggctcag gcgcccgcgc cccaacgcca ggaagccgcc gccgcagctc
     1621 tccgcagcgt cttgccggag gactgcgtcc tgtggcgcga agcggagcgg cgcccgtacg
     1681 agtgcgatgg gctgaccgcg tttcgccaga tcccaatgct ggttgcgctg cctcgcaccg
     1741 aagagcaggt gcatcaggtg ctcaggctct gcaatcggct ggacctgccg gtcgtcgcgc
     1801 gaggcgccgg cacgggtctc tccggaggag ccatgcccca cgcatccggg gtgctcctct
     1861 cgctcgcacg tctcaatcgc atcctggaaa tcaacccgct ggcccgtcag gcccgggttc
     1921 aaccgggcgt gcgcaatctg caggtatcgg aggcagcagc aagttacggt ctgtattacg
     1981 cgcctgaccc ctcatcccag atcgcatgct ccatcggcgg caatgtggcg gagaactccg
     2041 gcggtgtgca ttgcctcaag tatggactga ctatccataa cgtgttgcgt ttgcgcggct
     2101 tcaccatgga gggcgagctg ctcgagctcg gctcgagcgc cctcgatggc cccggtctgg
     2161 atctgctcgc caccgtcatc ggctcggaag gtctgctgat gatcgtgacg gaagtgacgc
     2221 tgaagctcat cccacgcccg cagctcgcgc aactcgtgat ggcctcgttc gatgatgttg
     2281 gcaaggccgg cgatgcggtg gccgccatca tttctgccgg catcattccg gccggcctcg
     2341 agatgatgga tcgcaaggcg acgcaggccg tggagccctt cgtgcgcgcc ggctatgacc
     2401 tgagcgccga agccattctc cttgccgagg cggacggtag ccccgaggaa gtcctcgagc
     2461 agatcgccga gatcgagcgc gtgttgcgcg cagcgggcgc aacagcgctc acggtttccc
     2521 actcggaggc cgagcggctg cgcttctggt cgggtcgcaa gaatgcattt ccggccgcgg
     2581 gacgcgtgtc ccccgattac tactgcatgg atggcacgat tccacgtaag aacctcggcc
     2641 acatgctcaa ggccatcgag cagatggagg ccgagcacgg gctgcgctgt atgaacgtct
     2701 ttcacgccgg cgatggcaac ctgcacccgc tcatcctctt cgatgcgaac caacccggtg
     2761 agtgggaacg cgcggatcgc ttcggcgcac agatcctcga gctgtgtgtg cagctcggag
     2821 ggacgatcac cggtgagcac ggggttggcg tcgagaagat caactccatg tgcgtacaat
     2881 tctccccgcg cgagcgcgcg gcattcttcg gcatcaagcg ggcattcgat cccagcgggc
     2941 tgctcaatcc gggcaaggcg attccgacac tcgcccgctg tgcggagtat ggaaagatgc
     3001 acgtccatgc cggcgccctc ccccacgccg acctgccgcg attctgagcc gatggaagcc
     3061 gagctgttga gattgcgcga gcaggtgctg atcgcccggc gcgatcgcac gcctctgcgg
     3121 ttacgtggcg caggcacgaa ggacttctac agcgcgagca actctcaatc gaccccgtgc
     3181 acgaccgtcg atctgcggcc cttcagcgga atcctcgact acgagccctc cgagcttgtc
     3241 atcaccgccc gttgtggcac gccgctgtca cagatcgagg cggccctggc cgaacgaggg
     3301 cagtttctag gcttcgagcc gcccggcttc ggcgccgacc cgacgttagg gggcatcgtg
     3361 gcggccggac tttcgggccc acgccgcctc agtgccggcg cggtgcgcga tttcgtactc
     3421 ggcgcagcgc tgctcgccgg cgacggcgag ctgttgcaat tcggtggccg tgtcatgaag
     3481 aacgtcgccg gcttcgacgt tgcccggctg ctgtgcggct cactcggtat cttcggcatc
     3541 atcacccagg tctgcatcaa ggtcctgcct cggccccgcg ccgaggcaac cgtgcagctg
     3601 gaaatgaagg cgggtgaggc gatcgaggcc ttcaaccgct ggggaagtcg gcctctgcct
     3661 gtctcggggg ccgcctggta tgacggtggc gcacggatcc gcttctccgg agcagtgccc
     3721 gctgttgaga aggcctgcac gcaaattggg gggaagcggc tcgatgcggc cgcggcacag
     3781 cggtggtggg actcgctgcg ccactacact cacccgctgt tctcctcaga ctcgctctgg
     3841 aggctgtcgc tgccccagac cgccgcgccg ctcgatctga gcggggatcc gctcatcgat
     3901 tggggcggcg cactgcgctg gtacgccggc gatcagggcg agcgtgacgt gcgagagctg
     3961 gcgcagcgcg cagcaggcac ggcgatgcgc tggcgggggc cagcagcaac cggtcggttt
     4021 catccactct ctcccgccgt ggcggaaatt catcgccgcc tcaagcgcgc gttcgatccc
     4081 gaaggcatct tcaatcccgg ccgcctgctc gcggatctgt aatgcagacg cgcctcgcca
     4141 gcgaattcag ggaccggccg gaagggcgta ctgccgatga gatcctgcgc gcctgtgttc
     4201 actgcggctt ctgtctggcg acctgcccga cctaccagct gctcggggat gagctcgaca
     4261 gcccgcgcgg acgcatctac cagatcaaac aggttctgga aggtgccccg ccgagccgcg
     4321 cgattcaggt tcatctggat cgctgcctga cctgccgggc ttgcgagacg gcctgcccgt
     4381 cgggtgttga gtatggtcac ctcgtagaca tcggtcgcgc cgaagtggat gctcgaacga
     4441 agcgccccct cgctgagcgg ctgtatcgct gggcgctgcg cgagggagtg agtcgcccgc
     4501 gcctgtttgg cttcgcgctg cgcaccggac gtgcggtgag cgggctcctt ccgaagcgtc
     4561 tgcgctcgaa actggggcac cgccgcccaa ccggctcctg gcccacccaa atccacgcgc
     4621 gcaaggttct actccccaca gcatgcacac agccctccct ctccccaccg atcgacttcg
     4681 ctacggcaag agtgctcgat gccgtcgggc tgcagagcat cgtcgtgcag ggggcaggct
     4741 gctgcggagc catccgccag cacctgagtg atcaggaggg cgcgcttgcg cagtgccgag
     4801 ccaacatcga cgcgtggtgg ccgtacgtcg agtcggaaca ggtcgaggcc attgtggtca
     4861 acgcatccgg ctgtgccgcg atgatcaagg aatacggcca tctgctgcgc aacgatccgc
     4921 agtactcgag caaagctgcg cacatcgtgc agagactgcg ggatcctgtg caggttctcg
     4981 cccctgagct cgagcgcttg cgcaagcgcc tctcgccgca aggtagtcgc cgcatcgcct
     5041 tccatccgcc gtgtactttg cagcacgcgc tgcagatccg ggggaaggtg gaagggctgc
     5101 tgagagcgct gggcgccgag cttgtgcccg tggccgatgc gcatctgtgc tgcggctcgg
     5161 cgggcactta ctccctctta cagtcggatc tcgccgaaga actgcgcacc cgcaagctcg
     5221 agaccttgct gcgcgagcaa cccgacctgg tgctctcggc caacatcggc tgcatcgcgc
     5281 atctgcaggg ggcctgcgct gttccggtcc ggcactggct ggaatgggtc gatgcggccc
     5341 tcactgccgg ccggacgccc cccatgtcgc aaacaggcaa gaaagaggta agataggtcc
     5401 ccgtgcgcgg gaaccagacc gagttcgctt ttctgacgat gccccggtat tcgatgatcg
     5461 ccctgtcgaa tgcggtcgag ccgctgcgca tggcgaacat ccagctcggc agaccggctt
     5521 acgcctggtc catcaccagt ctcgacggcc agccgacccc ggccagcaat ggcctgcagc
     5581 tcacaccgac cgtgccgctg gagtcgatcc gctccgtcga tatcctgttt gtgtgcggcg
     5641 gcgtcgatgt tcagcaaggg gtttcacccc ggctcctcgc cgccctccgt cgcttcgctg
     5701 accggcggat cccgctcggc gcgctgtgca ccggaggcta tgcgctcgcc cgggccggat
     5761 tgctcgacaa gtaccgcgcc acgattcact gggagaacct ttcggcccta cgcgaggagt
     5821 tcccccgcat cctgctcaac gaccagcttt tcaccatcga ccgcgatcgc ttcacctgct
     5881 ccgggggcgt ggcaccgctc gatcttatgt tgcatctggt gaaggccaaa catggcgcgc
     5941 gcatctcgca gttgatctcg gagcagttca tcgtcgaccg agtgcgcaac gatcgcgacc
     6001 gccagtacgt gccgctgcgc gcgcagatcg gggtgagcca cgacaccctg atccgtgtag
     6061 cgcagctgat ggaacagaat atcgagaagc cgctgtcgct cgacgagatt gccgcggcca
     6121 cggagctctc ccgccggcag atcgagcggc tgttcaagcg ccatctgggc tgcgtcccga
     6181 agcgctacta cctgcagatg cggctgcggc gtgcccgcga gttgctgttg cagacctcca
     6241 tgccgataat cgacataacg acggcctgcg gcttccaatc cccgccgcac ttctcgcgct
     6301 gctaccgggc gcagttcggc tgcccgccga gcgctgagag gcaaattcgc cacaccaagg
     6361 cgggctccgg cctgcggacg cgtagcgatg tcctgtcgtg atcgcgatgt cgtgcgcgga
     6421 cacgccgtgg tcgctaagtc acaaccggat cgtaaccccc ccacttaaca ttcccgcagg
     6481 agtcggcccg cgcagtccgc ccaccgcgcg tgcctggaga atcgagcatg gcagagcagg
     6541 cgcaagccga gttcaatcta cgcgacctcg atgatcagga gctgatcgaa caggttcacg
     6601 atgatctgta caacggactg aagagcgagg tcgaagaggc cacgcacatc ttcctcgagc
     6661 gaggctgggg cgcggagaaa gtcctgaacg aggcgctcgt ggagggaatg cgcatcgtcg
     6721 gcatcgactt ccgcgacggc atcctgttcg tgcccgaagt gctgctcgcc gccaactcga
     6781 tgaagggcgg catggagatc ctgcggccgc tgctggcaga gaccggggtc gagccgatcg
     6841 gcaagatcgt gatcggcacg gtcaagggcg acattcacga catcggtaag aacctcgtct
     6901 cgatgatgct cgagggcgcc ggcttcgagg tgatcgatct gggcatcaac aatccggtcg
     6961 agaagtacct cgcagcgctc gaggagcaca agcctgacat cctggggatg tcagcgctgc
     7021 tgacgacgac catgccgtac atgaaggtgg tcatcgaccg cctgaaggaa caggggatcc
     7081 gcaag
//
LOCUS       DASRHN010000006         6030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1814696, whole genome shotgun sequence.
ACCESSION   DASRHN010000006 DASRHN010000000
VERSION     DASRHN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814696"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..210
                     /locus_tag="VFB37_00185"
     CDS             16..210
                     /locus_tag="VFB37_00185"
                     /inference="COORDINATES: protein motif:HMM:NF021366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 family protein"
                     /protein_id="HZO20885.1"
                     /translation="MSVNFSDLLAALALCFVLEGIATFLHPRGVKRAFARLLEVTDRE
                     LRIAGLGSMLVGIVILFLVR"
     gene            222..1517
                     /locus_tag="VFB37_00190"
     CDS             222..1517
                     /locus_tag="VFB37_00190"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZO20886.1"
                     /translation="MGKFVVVIGTQWGDEGKGKVVDLLTEHAVAVARFQGGHNAGHTL
                     VIGARKTILSLIPSGILRENVRCFIGNGVVLSLEALMAESAMLMERGVPVFDRLRLSP
                     YCPLILPSHVELDKARERARGPNAIGTTGRGIGPAYEDKVARRAVRVADLFQRDRFAA
                     RLGEVLDFHNFVLKDYFRQPPVDFQKTLDQQLALAEHIAPLVTDVTLALHSLRQGGAN
                     VMFEGAQGAMLDVDLGTYPYVTSSNTTAGYAGTGTGLGPRAFDEVIGIVKAYTTRVGA
                     GPFPTELFDEYGEHLSRVGHEFGSVTGRRRRCGWFDSVALRRAIVHSSVSSLCITKLD
                     VLDGLDTIRVGIGYRIGGEVVAQPPLGIDGYAGVEPVYEELPGWTESTVGITDYEALP
                     VNARRYLERLQALVSVPIGLISTGPDREHIIVLRHPFAD"
     gene            1520..3256
                     /locus_tag="VFB37_00195"
     CDS             1520..3256
                     /locus_tag="VFB37_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO20887.1"
                     /translation="MARAGLALLILLSCLLAASTWRVYGHTWDEPEHLAAGLQLLDRG
                     RYEYDTEHPPIARVLMALGPWLAGAHSFGTPPPDGVQEGKDILYAGGHYQRTLTLARL
                     GVLPFLIVLLVAMWLWARQVARTEGEALLAVGLLASVPPVIGHAALATLDVPGTATTL
                     LALYLMQRWLARGALLDSALFGFVAGVAACTKLSAVPFIGLGLIVSAGLSGTLRWRGG
                     PRRQSGVQESGAQKSGDQTPASAWTRYGLGALILLLSLAGAIVLAYGWRSIELTTVPA
                     DREWILSYLFPAAPTSGLAAAISHVQLPEALWDFAEGILAVKAHNDAGHVSFLLGELR
                     EGGWWYFYLVALAVKTPLPLLLTGPAGLALLAREGWRRCDPWRLAPASLFVSLLAFAS
                     FFSRINIGIRHVLILYPFLALGAASLLAWIWRWGRTHRGGRWGVVAAAGVLGLMGWQV
                     SALWRAAPDYLPYFNEMVPHPERVLVDSDLDWGQDLRRLERRLAQLRVRYVSLAYLGT
                     ADLSQEALPTFALLPPEKPVSGWVAITALAREHAPLGYAWLNAYRPLERVGKTIDLYF
                     IPPPARAPGGSS"
     gene            complement(3216..4499)
                     /locus_tag="VFB37_00200"
     CDS             complement(3216..4499)
                     /locus_tag="VFB37_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZO20888.1"
                     /translation="MRWLTYLFVCALLAGVLIRFWLSTRQIKAVCAHRQRVPAPFTDR
                     ISLADHEKAADYTVANARLGQVSVALDALVTLGFTVAGGLAAIDGLWARAGWPQLWRG
                     AAVILTMACLSALLDLPLSVWRTFKLESRFGFNRVTPRLFLTDLAKSAALAVALGGPV
                     VVGALLLMERAGRLWWVWAWCGWLGLTLLMTWAWPAFIAPLFHRFTPLEDPALRARIE
                     SLLQRCGFASQGIFVIDGSRRSSHGNAYFTGLGRNKRIVFFDTLLSRLTHSEVEAVLA
                     HELGHFRLRHVRKRLLVTMAVSFGALALLGWLAGQPGFYRALGVSAPSAHSALVLFVL
                     AAPVFLFFATPLGSLWSRSHEFAADAFAAQQASPGELAVALVKLYRDNATTLTPDELY
                     SRFYYSHPPALERIRRLRQLDALSSTHPAPAPAAG"
     gene            complement(4669..4821)
                     /locus_tag="VFB37_00205"
     CDS             complement(4669..4821)
                     /locus_tag="VFB37_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HZO20889.1"
                     /translation="MLHYAIVFFIIAIVAAILGFGGIAAGAAGIAKVLFFVFLVLAIL
                     TFLRRA"
     gene            5421..>6030
                     /locus_tag="VFB37_00210"
     CDS             5421..>6030
                     /locus_tag="VFB37_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZO20890.1"
                     /translation="MKANQKVYCAVAAILSAHALAATAATPADATPADEESTTGITEV
                     VVTAQRRSENIQDVPIAIQALTSDTLKQLNVQTFDDYVKYIPSVTFKSNGPGQSLIYM
                     RGLSSGASAGSQSTGTIGGFPNVAIYLDDQSGQLPGRNLDIYAADLERIEVLEGPQGT
                     LFGAGAEAGVIRYITNKPKLNTTEASTTASYGTTAHGDNNSSI"
BASE COUNT         1009 a         1906 c         2038 g         1077 t
ORIGIN      
        1 gcgcaaggct gacgagtgtc cgtcaacttc tccgatctgc tcgcggccct ggcgctgtgt
       61 ttcgtgctgg aaggcatcgc cacttttctg catccgcggg gagtcaagcg tgccttcgcg
      121 cggctgctgg aagtcaccga ccgtgagttg cgcattgccg ggctcggcag catgctggtg
      181 ggcatcgtca tcctgttcct ggtgaggtag aggcgcatcg cgtgggcaag ttcgtcgtcg
      241 tcatcggcac acagtggggc gatgaaggca agggcaaggt cgttgacctg ctgaccgagc
      301 acgctgtggc ggtcgcccgc ttccagggcg gccacaatgc cggtcacacg ctggtgatcg
      361 gtgcccgcaa gaccattctg tcgctgatcc catccggcat cctgcgggag aacgtgcgct
      421 gcttcatcgg caatggcgtc gtgctatcgc tcgaggcgct gatggccgag agcgcgatgc
      481 tcatggagcg cggcgtgccg gtgttcgatc ggctgcggct ctcgccttac tgcccgctca
      541 tcctgccctc gcacgtggag ctggacaaag cgcgcgagcg ggctcgtggc ccgaacgcca
      601 tcggcacgac cggccgtggc attggacctg cctacgagga caaggtggcg cggcgcgcgg
      661 tccgggtggc cgacctgttc cagcgcgatc gcttcgccgc ccggctgggc gaggtgctgg
      721 atttccacaa cttcgtgctg aaggactatt ttcgccagcc gccggtggat ttccagaaaa
      781 ccctggatca gcaactcgcc ctggcggaac acatcgcacc gctggtcacc gatgtcacgc
      841 ttgcgctgca ctcgctgcgc caagggggtg ccaatgtcat gttcgaaggc gctcagggcg
      901 cgatgctgga tgtggacctc ggcacgtatc cctatgtcac ctcttccaac acaacggccg
      961 gttatgccgg cacgggtacg ggtctgggtc cccgcgcgtt cgatgaagtg atcggcatcg
     1021 tcaaggcata cacgacacgc gtcggcgccg gtccttttcc caccgagctt ttcgatgagt
     1081 acggcgagca tctgtcgcgg gtcggccacg agttcggctc ggtgacgggc cgccgccgcc
     1141 gctgcggctg gttcgactcc gttgcgctgc ggcgcgcgat cgtgcactcc agcgtctcga
     1201 gcctgtgcat caccaagctc gatgtgctcg atggtctgga cacgatccgc gtgggtatcg
     1261 gctaccgcat cggcggcgaa gtcgtggcgc agccgcctct gggcatcgat ggctacgcgg
     1321 gcgtcgagcc ggtgtacgag gagcttccgg gctggacgga gtccaccgta ggtatcacgg
     1381 attacgaagc gttgcccgtc aacgcccgtc gctatcttga gcgtctgcag gcactcgtga
     1441 gcgtgccaat cggtctgatc tccaccggcc cggatcggga gcacatcatc gtgctgagac
     1501 atccgttcgc cgactgacct tggctcgcgc cggcctcgcg ctcctcatcc tcctctcgtg
     1561 cttgctcgcc gccagcacct ggcgggtcta cgggcatacg tgggacgagc cggagcatct
     1621 agcggcgggg ctgcagctgc tcgatcgcgg ccggtacgaa tatgacaccg agcatccgcc
     1681 gatcgcgcgc gtgctcatgg cgctgggtcc ctggctcgcc ggtgctcact ccttcggcac
     1741 gccgccgccc gatggcgtgc aggagggcaa ggacattctc tatgccggtg gccactacca
     1801 acgaactctg acgctggccc ggctcggtgt gttgcccttc ctcatcgtgc tgctggtggc
     1861 catgtggctc tgggcacggc aagtcgctcg cacggaaggt gaagcgctcc tggcggttgg
     1921 gctgctggca tcggtgccac cggtcatcgg ccacgctgcc ctcgccacgc tggatgtgcc
     1981 cggcactgcg accacgctgc ttgcgctgta cctgatgcag cgttggctcg cgagaggcgc
     2041 gcttttggat agcgccctgt tcggattcgt cgcgggtgtc gcggcctgca ccaagctgtc
     2101 ggccgtgccc ttcatcgggc ttgggctgat cgtctcggca gggctgagcg gtacgctgag
     2161 gtggcgcggc gggccgcgca ggcagtccgg cgtgcaggag tctggtgcgc agaagtctgg
     2221 tgatcagaca ccggcaagcg cctggactcg gtatggcctt ggcgcgctga ttctgctgct
     2281 cagcctggcg ggtgcgatcg tgctcgctta cggctggcgc tcgatcgagc tgaccactgt
     2341 acccgcggac cgtgagtgga tcctgtccta cctttttccg gccgctccca ccagcggcct
     2401 ggccgcagcg atctcacacg ttcaactccc cgaggcgctg tgggattttg ccgaggggat
     2461 tcttgcggtg aaggctcaca atgacgccgg acatgtttcc ttcctgctgg gtgagctgag
     2521 ggaggggggt tggtggtact tctacctggt ggcgctggca gtcaagacac ctctgccgct
     2581 gctgctgacc ggacctgcgg gtctggcgct gctggcgcgg gagggctggc ggcggtgcga
     2641 cccttggcgt ctggcgccgg cgagcctatt cgtgagcttg ctcgcctttg ccagcttctt
     2701 cagccggatc aacatcggga tccgccatgt cctgatcctc tatcccttcc tcgccctcgg
     2761 cgcagccagc ctgctcgcct ggatctggag atggggccgt acccacaggg gtgggcgctg
     2821 gggggtcgtc gcagccgcgg gggtgctggg gctgatgggc tggcaggtga gcgcactgtg
     2881 gcgggcggcg cctgactatc ttccgtactt caatgagatg gtgccgcatc ccgaacgggt
     2941 gctggtggac tcggacctgg attgggggca ggacctgcgg cggctggagc ggcgactggc
     3001 gcagctgcgg gtccggtacg tcagtcttgc ctatctgggc acggcggatc tctctcagga
     3061 agcgttgccg acgtttgcgc tgctgccccc ggaaaagccg gtgtccggct gggttgcgat
     3121 caccgcgttg gcccgggaac acgcgcccct cggctacgcg tggctgaatg cttatcggcc
     3181 acttgagcgc gtaggcaaaa cgatcgatct gtatttcatc ccgccgccgg cgcgggcgcc
     3241 gggtgggtcg agctgagcgc gtcgagctgt cgcaagcgtc tgatgcgctc cagcgccggc
     3301 gggtgcgaat agtagaaccg cgaatacaac tcatccggcg tcagggtcgt ggcgttgtca
     3361 cggtaaagtt tcaccagcgc gaccgccagt tctccggggc ttgcctgttg cgccgcgaag
     3421 gcatcggcgg cgaattcgtg cgatcgggac cagagggagc caagcggggt ggcgaagaaa
     3481 agaaacacgg gcgcggccag gacaaaaagg accaacgccg agtgtgcgga aggcgcggac
     3541 acgcctagag ccctgtagaa gccgggctgc ccggccagcc atccgagcag cgccagcgcc
     3601 ccgaacgaga ctgccatcgt gaccagcaga cgtttgcgta cgtggcgcag ccggaaatgt
     3661 cccagctcat gggcgagcac cgcctcgact tcggaatggg tcaggcgcga aagcagggta
     3721 tcgaagaaca cgattcgctt gttgcggccc aagcccgtga agtacgcatt accgtgagat
     3781 gagcgccgcg atccgtcgat cacgaagatg ccctgggatg cgaacccgca gcgctgcagg
     3841 agggattcaa tgcgtgcgcg caatgccggg tcctccagag gcgtgaagcg atgaaagagt
     3901 ggggcgatga atgccggcca cgcccacgtc atgagcaatg taaggccgag ccagccacac
     3961 caggcccaga cccaccagag ccgcccggcg cgctccatca gcagcagcgc gcccactacg
     4021 acggggccgc cgagggcgac cgcgagcgca gcgctcttcg ccagatcggt caggaagagt
     4081 cgcggcgtga cgcggttgaa gccaaaacgg ctttccagct tgaacgtccg ccatactgaa
     4141 agaggcaggt ccagcagcgc actgaggcag gccatcgtca gaatgactgc tgcccccctc
     4201 cagagctgcg gccacccggc gcgggcccac aggccatcga tggctgcaag accgcctgcg
     4261 acggtgaatc cgagtgtgac gagcgcatcc agcgccacgg agacctggcc gaggcgggcg
     4321 ttcgcgacgg tgtagtcggc ggccttctca tgatcggcca gggagattcg atcggtaaag
     4381 ggtgcaggca cccgctgccg gtgcgcgcac accgctttga tctgccgtgt cgaaagccag
     4441 aagcgaatga gcacccctgc caggagggca cacacgaaga ggtaggtcag ccagcgcatg
     4501 cctggcgatt atgccttgga cgcgctccga gggcccagcc gccgccgggc acgctgacag
     4561 ccccgcgcca gcgcggcctg gcaggggcgt gttgcgccac cacagggaga gccccgcgac
     4621 tctggctcaa tgcttcagag ccgcgaggat tattgtcgat cggtaaacct acgcgcggcg
     4681 caagaaggtc aggatggcaa gcaccaggaa tacgaagaac agcaccttcg cgatcccggc
     4741 cgcgcctgcc gcaatgccgc cgaacccgag gatcgccgcg acaatggcga tgatgaaaaa
     4801 gacgatcgca tagtgaagca tttagttctc ccggcgcccg catctttacg gcggtgactc
     4861 agtagaagcg atgctgagcc gtcggctcat cgattgttat cggagtccgc cccgcttccg
     4921 tgtgggaggc gtccgacgag gcttgcgcga atgcgcgcgg ggcctgcact ttcgcggcgc
     4981 atcgctcctc gctgggtccg gctgctctga ccgatcgcgg cgccagaggg gttgcggcga
     5041 gctgctgccg cgtccataca gcacagatgt cgccgccata catcgctcga gcggcgatct
     5101 catgttgcga tggcgcatcg cgcggatgtg cctagaagtt gtggacaagc gggccgggtg
     5161 cccgcaggat gcgatacgtg cggcgcgagt cgcatgcggg gcggtgattg gattcccagt
     5221 gcaggaatcg aagaaggcca tgattttttt ctatggcccg atccaatttc tccgcttgcc
     5281 tgggttgatg tgactgtgaa aagtttgggc ggggggcctt cggccgcaag tctcgtgttg
     5341 cccgtgcgct acagcacggc accgggctgg aaccacggcg gctctcccgg gacgacagcc
     5401 aacattcggg ggagagcagc ttgaaagcga accagaaggt ctactgtgcc gttgccgcca
     5461 tcctgagcgc gcacgcgctg gccgcgaccg ccgccacacc cgcggatgcg actcccgcgg
     5521 acgaggaatc caccaccggc atcaccgaag tcgtggtgac tgcgcagcgg cgctcggaaa
     5581 acatccagga cgtgccgatc gcgatccagg cgctcacgtc tgacacgctg aagcagctca
     5641 acgtgcagac cttcgatgac tacgtgaaat acatcccgag tgtgactttc aagtccaacg
     5701 gcccggggca aagtctgatc tacatgcgcg gcctgagctc gggtgcatcc gcgggcagcc
     5761 agtcgacggg cacgatcggc ggattcccga atgtggccat ctacctcgat gatcagtccg
     5821 gtcagctgcc tggccgtaac cttgacattt atgcggcgga cctggagcgc atcgaggttc
     5881 tggaaggtcc gcagggcacc ctgttcggcg ccggcgcgga agcgggtgtg atccgctata
     5941 tcaccaacaa gcccaagctc aacacgaccg aggccagcac gacggccagc tacgggacca
     6001 cggcgcacgg tgataacaac agttccattc
//
LOCUS       DASRHN010000007         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1815162, whole genome shotgun sequence.
ACCESSION   DASRHN010000007 DASRHN010000000
VERSION     DASRHN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815162"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFB37_00215"
     CDS             complement(<1..376)
                     /locus_tag="VFB37_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZO20891.1"
                     /translation="MGASAGQLLAIGFDGVELSPPLASLLTRIQPAGVILFSRNIVNA
                     QQTHKLLKDCQFTAREPLFTCVDVEGGRVDRLRGVFGPAPSAAEVFASRRKKLYRQHG
                     ELIGRACRALGFNVDLAPVLDLA"
     gene            complement(551..>2513)
                     /gene="mutS"
                     /locus_tag="VFB37_00220"
     CDS             complement(551..>2513)
                     /gene="mutS"
                     /locus_tag="VFB37_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZO20892.1"
                     /translation="HFGVMSLEGFGLAGKPAAATAAGAILYYVRSTQRGTLEHVDRIG
                     FYERQNCLVLDAVTVRNLELIEPLFAGTGAGVTLFRSLDATVTPMAKRLLRAWMLRPS
                     IERSEIESRLDAVEEGAGQTIAREELRRSLEGVLDLERLLSRVTLETANPRDLVALAA
                     SLARVPALRAALSRLHSPRFASLHSAIDELADVRERIEKTIVPEPPVSLAEGGVIQRG
                     ADRELDELHQLSRNSKQFLAEMELRERERTGIQSLKVRFNSVFGYYLEVSKPNLHLVP
                     PDYERRQTLVNAERFTTPELKQYETRILDAQERMVEIERRIFSGLRATIAGEARRIRQ
                     TATAIAEVDVLASFAHVAALRNYCRPQLDESSDIEILAGRHAVIEQQELAGSERFVPN
                     DLYLNSTTHTILVLTGPNMGGKSTYLRQAALMVILAQMGSFVPAAKCRLGVVDRVFTR
                     IGASDNLARGRSTFMVEMTETAAILHTATPRSLILLDEVGRGTATYDGLAIAWAAIEY
                     LHSRTRAKTLFATHYFELTELAGQLPGVKNYHVSVKETPTGIVFLRKVEPGAADRSYG
                     IEVAKLAGLPEEVVGRAREVLAEHENAETRMSAHLAGGEAPAARRAQLTIFTPISQPV
                     LERLRDLDLNRLTPLEALNLLAELKQQVQ"
BASE COUNT          447 a          785 c          860 g          421 t
ORIGIN      
        1 gcgccagatc gagcaccggg gcgaggtcca cgttaaagcc gagcgcccgg caggccctgc
       61 cgatcagctc gccgtgctgg cggtacagct tcttccgccg gctggcgaat acttccgcgg
      121 ccgagggcgc gggaccaaag acgccgcgca agcggtccac ccgcccgccc tcaacgtcca
      181 cgcaggtgaa cagcggctca cgcgcggtga actggcagtc cttcagcagc ttgtgcgtct
      241 gctgggcgtt gacaatgttg cgggaaaaga gaatcacccc tgcaggctgg atgcgggtga
      301 gcagcgatgc cagcgggggc gagagctcga ccccgtcaaa cccgatcgcc agcagctggc
      361 cggcgcttgc gcccaacccg gaggaggatt ttcctggcat gagagtaaga ggctatttta
      421 cgtgagtcct gcctggcgag aatggcaagc aacttccctt tcgcggggtt tacacatgtg
      481 aacacaaatc aaaacatcaa tcggagaaag cggcattcgt ccgcatgaat cgggtggaga
      541 aagaattctt tcactgcacc tgctgtttca gctccgccag caggttcagg gcctcgagcg
      601 gagtcagacg attgaggtcc agatcgcgca ggcgctcgag caccggctgc gagatcgggg
      661 taaagatcgt aagctgcgca cgacgcgctg caggggcttc gccgcccgcg agatgcgcgc
      721 tcatccgtgt ttcggcgttc tcgtgctcgg ccagcacctc gcgggcgcgg ccgacgacct
      781 cctcgggcag cccggcgagt ttcgcgacct cgataccata actgcggtcg gcggcgcccg
      841 gctccacctt gcgcaggaaa acgatgccag tgggggtttc ttttaccgag acatggtagt
      901 tcttcacccc cgggagttgc ccggccagct ccgtcagctc gaagtaatgc gtggcgaaga
      961 gcgtcttggc gcgcgtgcgg gagtgcaggt attcgatcgc agcccaggcg atggccagcc
     1021 catcataggt tgcggtgccg cgcccgacct cgtcgagcag gatgagcgag cgcggcgtcg
     1081 cggtatggag gatggccgcg gtctcggtca tctccaccat gaaggtcgag cgtccacggg
     1141 ccagattatc gctggcaccg atgcgggtga agacgcggtc cacaactccc aggcggcact
     1201 tcgctgccgg aacgaacgag cccatctgag ccagaattac catcagcgcc gcctgccgca
     1261 ggtaggtgga cttgccgccc atgttcggcc cggtgagcac caggatggtg tgcgtggtgg
     1321 agttgagata aagatcgttg ggcacgaagc gctcgcttcc ggccagctcc tgctgctcga
     1381 tcacggcatg gcgtccggcc aaaatttcga tgtcggagga ctcatccaac tgcggccggc
     1441 agtagttcct gagcgccgcc acgtgggcaa aggaggccag cacgtccacc tcggcgatgg
     1501 cggttgcagt ctggcggatg cgccgggcct cgcccgcgat cgtggcccgc agcccggaga
     1561 aaatccggcg ctcgatctcg accatccgct cctgggcgtc gaggatcctg gtttcgtact
     1621 gcttgagctc gggggtggtg aagcgctcgg cgttgaccag ggtctggcgg cgctcgtaat
     1681 ccggcggcac caggtgcagg ttgggctttg aaacctccag gtaatagcca aacaccgagt
     1741 taaagcggac ctttaaggat tggatcccgg tgcgctcgcg ttcgcgcaac tccatctcgg
     1801 ccaggaactg cttggagttg cggctgagct ggtggagctc atcgagctcg cggtcggcgc
     1861 cgcgctggat gaccccgcct tcggcgagcg agaccggagg ctcgggaacg atggtcttct
     1921 cgatacgctc gcggacatcc gccagctcgt ctatggcgct gtgcagggac gcgaaccggg
     1981 gggagtgcag ccgggaaagc gcggcgcgca gggcgggaac gcgggcgagc gaggcggcca
     2041 gcgccaccag atcgcgcggg ttcgcggtct caagcgtcac ccggctgagc aggcgctcga
     2101 gatcgagcac gccttcgagc gagcgccgca gctcttcgcg ggcgatggtc tggcccgcac
     2161 cctcttcgac cgcatcaagg cgcgattcga tctcgctgcg ctcaatcgag ggccgcagca
     2221 tccaggcgcg cagcaggcgc ttggccatgg gggtgacggt ggcatcgagc gagcggaaca
     2281 gggtgacgcc ggcgccggtc ccggcaaaca gcggctcgat gagctcgagg ttgcgtaccg
     2341 tgaccgcatc cagcaccagg cagttctgcc gctcgtaaaa gccgatgcgg tccacatgct
     2401 ccagggtccc gcgctgggtg ctgcggacat agtaaaggat ggcgccggct gcggttgcgg
     2461 ccgcgggttt gccggccagg ccgaagcctt cgagcgacat caccccaaaa tgg
//
LOCUS       DASRHN010000008        21988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1321096, whole genome shotgun sequence.
ACCESSION   DASRHN010000008 DASRHN010000000
VERSION     DASRHN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321096"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..511
                     /locus_tag="VFB37_00225"
     CDS             164..511
                     /locus_tag="VFB37_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20893.1"
                     /translation="MTFASATPASRQNMLGDVVGSGWVTRCGTLLRQLGPYAAIELLL
                     PGGSLIVLALWLYRRHRLGRREDAQSKMIARVACAILTAVTAPLSFRAVADAAVQCPP
                     PRLAVQARAYTLA"
     gene            659..2539
                     /gene="zorA"
                     /locus_tag="VFB37_00230"
     CDS             659..2539
                     /gene="zorA"
                     /locus_tag="VFB37_00230"
                     /inference="COORDINATES: protein motif:HMM:NF033916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage ZorAB system protein ZorA"
                     /protein_id="HZO20894.1"
                     /translation="MLKLLPYFQANPLVSILGLLVFVLLLHFLVQYISRGLALRAQLI
                     RLVEEIGTSGAPGSAEMKSQLAQLFRKTRLARAWREFEESLHVQDAEVGAGPSAAPIR
                     STQSAEVFFNLETVIDPWIGSEYFRHLPGILTGLGIIGTFFGLIQGLVHFDPSLSDSA
                     GLRRGLGELFGHVRDAFMFSGSAIAAAIAVTGAEKWLYASCAKWVFELAAALDAQFKS
                     GVGEEYLASILRASQDSAVQVRNLKTVMAEDQRSLLTYLADRQLQATQQMAAEIGRSI
                     RQSLQEPLADIARTVALVSGRETEHLKTALEQLMTSFLEQMRETMGRQMGDLSSLLQQ
                     SARAVHQLEIAMQDLLQDVQQSGHDSVASMRTAVQELVQKLSETQRAQSDLVSSAMKD
                     VLGQLKEATVRMALAQEEAARRAREGNQAAEAHMRRQVATLADSHMTTIGATRELLER
                     FNGSSAAMIDKLSLGTVSLVSAMKSVQQSAEQLSRVGLELAALEGQAQRSSQDMVRAS
                     SHLALAAQNVGNSIHQLSNAAVRFEGVASSAGVEANARRQILVSLQDLIDQSQIASRE
                     FVNLAHEARKALDAGVERFDSEVSTVLSGHVRTYQKQLGDSVSGLRRALEQLAARTRD
                     RT"
     gene            2536..3270
                     /locus_tag="VFB37_00235"
     CDS             2536..3270
                     /locus_tag="VFB37_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20895.1"
                     /translation="MSVLQQLIRPARRRPEAERPFWISYADLMTAMMTLCLVAMAVTI
                     IAMSQELRRELSAEDTRAQEILGICNEIKQGIRDRPNVHVDCRDNRIDFGEAGQFGYE
                     EYRLHPEVESDLAALVPVILRASNDRDGRKWFKQVVIEGFTDTRGPYLYNLYLSLKRS
                     YWMMCMLVDPRRNASLGLSDEQVQQVRELFLAGGVSFNDAKPSDDASRRVELRLQFFT
                     RKEKEEPDQAARPHFITTQQESCELN"
     gene            complement(3236..4312)
                     /locus_tag="VFB37_00240"
     CDS             complement(3236..4312)
                     /locus_tag="VFB37_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO20896.1"
                     /translation="MSLIPRSLVGRTAVFVTLIVMANHILWFSVVRPMVFNRYVQADQ
                     VMHPAGVLRLYVELEWAAFALLTSAAGIYVIFFWLRRQLASVVQAARVMGSGQKPEAL
                     PDGGPSEIRQLSRGFNQLALNLEALESERRLMLVGISHDLATPLTRLRLALELLDMRG
                     SPADIQGMLQDLEEMSAILQQFTDYAKTGREEPPVAGDLGALAAEVCSRYRAVGRNIR
                     MQLIPGPTFMFRPLAMRRLITNLLDNAIRYGGQDVEIHLERTDERIVLSVLDRGPGIR
                     SVDPNALIRPFAREDPSRGSHTGAGLGLAIVERIAIAHGGGLHLHNRADGGLAAVVTL
                     AAPGSSPSGRQPRLSSARSFPAVS"
     gene            complement(4309..5055)
                     /gene="ompR"
                     /locus_tag="VFB37_00245"
     CDS             complement(4309..5055)
                     /gene="ompR"
                     /locus_tag="VFB37_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HZO20897.1"
                     /translation="MSITLTAVMKPVSARILVVDDDRRTRSLLQSYLQEMGFQVSVEP
                     DGTQLEHVLQTAAVDLLVLDLMLPGEDGLSLCRRLRGKGVEVPIIMLTAKGDDIDRIV
                     GLELGADDYLPKPFNPRELVARVKAVLRRRPEVPVGAPSAHEGTVRFGPCELNLGTRS
                     LARSGERLPITTGEFAVLEALVRHPHKPLSRERLMEIARGREHGAYDRSMDVQISRLR
                     RLIEEDSSKPRFIQTVWGFGYVFVPDPSPA"
     gene            5221..5802
                     /locus_tag="VFB37_00250"
     CDS             5221..5802
                     /locus_tag="VFB37_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZO20898.1"
                     /translation="MRGLFYATVVLASAGVTASPVTYTIDPDHTHPAFEADHLDGLSV
                     WRGIFRTTHGRITLDRAAQSGSVQIEVEVASVDFGNEQLNETAAHASAPPIFEAQRYP
                     TARYEGKLTQFVRGVPTRVTGTLEMHGVSRPLQLKIDSFKCLQHPLRKREVCGADASG
                     TLNRADFGIKVGQGYGFRMGVRLHIQVEAIRAG"
     gene            5896..6969
                     /locus_tag="VFB37_00255"
     CDS             5896..6969
                     /locus_tag="VFB37_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO20899.1"
                     /translation="MLAALVLAWLLRAPRTSAISAPDEALPVATAIAFQQSMPVLVEA
                     VGEVISRHSVQIRPQITGTLRRVCFSEGQEVVAGQRLFEIDPAVFTAAAAAARAAWES
                     ARANAERLPPLAKQGYVTPQDLLNARSATDQAEAAYKQAAINLSYTQLRAPIAGRTGV
                     LSRKAGNIVAPSDAAPLVTINEIRPIEVQFSIPQQTLGAVRQQLAAHAISVRISSEDG
                     AETLDEGVLGFIDNTVNAQTGTILLKARTPNRSEQLWPGEYVGVHMELRVEPRAIVVP
                     ATAVQTGQEGPYVFLVTQGAAVARTVRVDREVGVLAVISSGLTAGQRVITRVPRGLRA
                     GMRVRPQDVAVGSRATVTVPESP"
     gene            6966..10079
                     /locus_tag="VFB37_00260"
     CDS             6966..10079
                     /locus_tag="VFB37_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO20900.1"
                     /translation="MNISVAFIRRPVMTTVLSFALVACGTVAYLRLPVSELPNVDFPT
                     ISVSASLPGANPKEMAATVATPLERRLGGIAGLIAMSSASTTGKTTIALQFALSRNID
                     AAAEDVQIAISQSSRQLPPNMPTPPTLSKVNPADNAIVYLALTGDRLPLTDLDEYAET
                     QIAEQIAEVPGVAQVQVFGSYQYATRIYLNPYALSARHLTLDMVSDAIRNSNTSLPSG
                     TLYGGTRTYTVESNGQLANAQAYNHIVIAERDGAPVHLNDVGKAVDGIQQDRQLTTFS
                     DATTGGELHPAVMLAIRRQPGANTVAVARGIQELLPRLTRQAPGDATLRLLYTRADFV
                     RDSVREVRNALLAAMGLVIAVVFLFLRRAGTTLISALSLPVSLLGTFVAMRLLGFNLD
                     HLSLMGLTLATGFIIDDAIVVLENIVRLRERGVPQLLAAESGTAEISSTVVSMTLSLV
                     AVFIPILLMGGMLGRLFREFAVTVSAAILLSGLVSLTLTPMLCAVFGAAGAERLRTPP
                     GMSRAQAWYRRSLVRSLAHWPSLLGAAAFMLVLAIGLFAWIPKGLMPSEDTGQIIGTT
                     LAPDGTTFEQLNDVQQNVARIVQRNPAVATVMSNAGQGWGSTGGNNLGLLYMGLKPVA
                     DRSSGAEVRAQLRQALAHVQGLDVFIENPSTLNLNSINSNAEFQYVLQSPGIDALYRE
                     APRFEQLLRQVRGLRDVSSDLSLNNPQLKVDLRREAASALGVTAEQVQSTLAEAYGGQ
                     QVSTIYGPANEYWVMMQLAPQYQTNPRALDALYVTGAGQNLVPLSAVAGISAGAGPVS
                     VSHYGQLPAVTLSFNLDPGTSLGVATERIETLARKSLSAQVTADFAGAAKSFTESSRE
                     LLLLLGLSVLVIYVVLAVLYEHFIHPITILTALPLAMVGGLLALLACGQELNVFSFVG
                     LVLLIGLVKKNGIIMVDFALQMRREQGLSASEAIVEACTVRFRPIMMTTLAAILGTLP
                     LALGSGAGIETRRPLGIAAAGGLLVSQFLTLYITPAFYLAMENLGDWYRRNCRGDRAD
                     PGGRHAL"
     gene            10270..10812
                     /locus_tag="VFB37_00265"
     CDS             10270..10812
                     /locus_tag="VFB37_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20901.1"
                     /translation="MNRKSRLAVALAWVTAVLMTVSAQASQSARHEAAELHRAKLSLI
                     EAIVTAEKQGAGRAVSASFEFKRGNPAYFEVKVLSPDGRKLTGYKLDPRTGQVQDTSN
                     EALEKLISRITPESLSRAPTTMTHAIALAQEHSGGHAVSADVDHDGDQINYTVETVKA
                     DGASQKLKVQGADGKVLSSD"
     gene            complement(10832..11281)
                     /gene="arsN2"
                     /locus_tag="VFB37_00270"
     CDS             complement(10832..11281)
                     /gene="arsN2"
                     /locus_tag="VFB37_00270"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HZO20902.1"
                     /translation="MNQFEVRPGPPLASAAALLGSVGLPAADLAERHLADFLFCGPAE
                     SPTGLVGLEIHGHDALLRSLAVAPESRRAGMGSALVQHAERHALGKGVRTVYLLTTTA
                     QAFFEDLGYGPTTRESCPLAIRATPEFVSLCPASAVILMKRLRWWGL"
     gene            complement(11308..12231)
                     /gene="puuE"
                     /locus_tag="VFB37_00275"
     CDS             complement(11308..12231)
                     /gene="puuE"
                     /locus_tag="VFB37_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HZO20903.1"
                     /translation="MSRDEAYPRDLIGYGAHPPHPRWPGEARVAVSIVLNYEEGAESC
                     VLHGDEHSESVLTDLGALEPLSGARNLNVESNFEYGSRVGFWEIMRVLHERGVQATIY
                     AVGMALERNPEAAAAMRQSGFEVACHGQRWIDYQFVAEDLERSHIEHNVEIITRLIGR
                     RPLGWYTGRPGPNTRRLVAEHGGFLYDSDAYNDDLPYWMRVAGQPYLIVPHAFDTNDS
                     RLQRGGDFASGEEFFTYARDAFEWLYRLGKEGRPRMMTLSLHGRIIGRPGRLGALARL
                     LDHIQAHEAVWVCSREALARHWIAHHPPPML"
     gene            complement(12228..13277)
                     /locus_tag="VFB37_00280"
     CDS             complement(12228..13277)
                     /locus_tag="VFB37_00280"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO20904.1"
                     /translation="MTLLITGGTGFVMSVLARRWLDGGRAQRAVVLDAAPLDAAARRY
                     FEPVSERLSVLVADVTQPATWRQGLARSEITEIVHGATITPISRGTAAEARREPEADD
                     PRRILDVNLMGTVAVLDWARTLPKLRRFIYVSSGAVYKHHGPDRPGEPLPEDGYVMPR
                     RLYGISKLASELITERYGDLFGVSTLSVRPSSVFGSMDRITSSRNFRHVPNRIAHMAV
                     EGVKCVRVNSLEAVGDYIHAEDVADAIIALLHAPALRHSVYNIAAGNTCSVADLVGWA
                     AEKSPGFSARIEPIETADIVVDPTLSSGMWGAYDISRIISETGWRPRPVREALHAYMD
                     WIAAERTVPQPEVQP"
     gene            complement(13295..14551)
                     /locus_tag="VFB37_00285"
     CDS             complement(13295..14551)
                     /locus_tag="VFB37_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZO20905.1"
                     /translation="MSGRSIQADLALAQLLFERLDRATRRGAGIVRDTYGPGEQAAHD
                     ILRAAAEQIGLEVNVDAIGNLMMVLPGRDRSRPRILIGSHLDSVPQGGNFDGAAGVVA
                     GIAVLSALRQDAFVPDCDLVVMGIRAEESAWFDVAYIGSAGALGLLDPTCLAVPRCDN
                     GCSLEQAIRQAGFDPAPIRERRPLLDPQTVRAYLELHIEQGPTLVNRGLPAAVVTGIR
                     GCKRLRNARCVGEYAHSGAVSRLYRRDAVAATVALLHHLEAFWLQQEAEGADLVMTSG
                     ELYTNPVLHGPSKIAGEVSFVIDVRSLADATMAAVIHEARRAAVALEQAYRVRFELGT
                     TSDSPPAVMDTRLRARLMGLLEQPFEMASGAGHDAAVFAQLRIPTAMIFVRNEQGSHN
                     PAEAMTLDDFAVGTRALLGLLLDFPL"
     gene            complement(14548..15324)
                     /locus_tag="VFB37_00290"
     CDS             complement(14548..15324)
                     /locus_tag="VFB37_00290"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO20906.1"
                     /translation="MSSLLAGKTAIVTGASSGIGRAIALTFAAEGAMVVIADLTTQAV
                     EGGDPTVELIKRAGGAAVFEKTDVGRWEDVDSLVARTVASHGRLDIMVNNAATYTGTP
                     LLETSPAQWDQVLGANLTGMFYGCKRAVEQMITQEPRNEVRGRLINLGSQQGIVNSPR
                     DTAYGVSKAGAIYLTRQIAVDYAKDLIVCNCISPGKIVTGKPGLAMDPALLEYARRRT
                     PWPRFGRPQDVANGALFLASDQASFITGSNLVIDGGWLAG"
     gene            complement(15349..16254)
                     /locus_tag="VFB37_00295"
     CDS             complement(15349..16254)
                     /locus_tag="VFB37_00295"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZO20907.1"
                     /translation="MNHPRFWLLTAAFIVSGAQAEGIRVLNSDAHFPEGPIWYHDKLY
                     YVEYDRNTVTTWDGSKNAVFWSEKGCGPSAVVPTARGELVTTCYDNGTIGHMSAEGKP
                     LPPYAHDKAGNAFLGPNDFAPDGHGGLYFTCSGTPGPIINGKVFYMAADGAIAQQAVD
                     LHSANGLAVSKDGRILYVVETEDHRLVEFDIAADRSLSNRRLFLNLDDMTNNVVHIYP
                     DGVKIDSKGQIYIGQNPRDVHAPLAGIIFVVDTSGHLLRKLALPSPGVPNLAFSPDEK
                     TVFVTALDQLDKSPYHGKIYSIPNR"
     gene            complement(16274..17584)
                     /locus_tag="VFB37_00300"
     CDS             complement(16274..17584)
                     /locus_tag="VFB37_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO20908.1"
                     /translation="MSVNAIAPPRSDTPSIIERWYVLIVMCMVYAINIAARYVVTTVY
                     EPIRLELKLTDAGAAFLTGVPLALFYVAFGIPIAWIADRSNRRNIVAASLVIWSAFTV
                     LCGLSQTYWQLLLARIGVGVGESGATPPSTAIVSDYFPAARRPMALSVLALGAPIGAW
                     LGANLAGAVAQAYGWRAAFFALGVPGVILGIVVFLSIREPQRGRLDVIAGEGKPSLLV
                     SLKFLWRQKAAFHVIMASGVCSLWGWGLAWWTPTFLLRSYGLNVAQAGALTGNIHLVG
                     GISAMLATVWLMSRPYMVDPRRVLWTLALVIGFATIPSFLTYWTHSLSVARLMLWLFI
                     PAIYFFIGPCMALVLNCAPSNMRSIFTAWSVLVGNLFNLIVAPQAVGLLSDWFAHGQA
                     ADAESLRLALLLLAPTGFWATWHFILAARTVTADQARAIGYSSA"
     gene            17805..18791
                     /locus_tag="VFB37_00305"
     CDS             17805..18791
                     /locus_tag="VFB37_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZO20909.1"
                     /translation="MASDFSTSAASRRALIFFDQADIFLPRLIERFPAIEFSACGAHD
                     ELPGMLNRIRPEIILAFKFEGRPFPRRELLACEALRWLSLAFAGVDVLMPWDDARLTV
                     TNAAGVAAEEMARYAFAAILGLFQGFPELHARQVKKSWQFHSHRSARDATLGLVGLGH
                     SGRAIARMARAIGLRVFACRNSAQPSEEVDAVFPREQLHEMLGRVDVALLCAPLTPLT
                     RDLFGAAAFAAMKPGSFFINMARGAMVEESALIEALLTGHLAGAVLDVTRTEPLPASD
                     PLWDAPNLLITPHSSSEYDGWISDAALMFADNLDRWIHARPLQNRVHPDRGY"
     gene            complement(18842..20035)
                     /locus_tag="VFB37_00310"
     CDS             complement(18842..20035)
                     /locus_tag="VFB37_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO20910.1"
                     /translation="MTELGLPQRLAGGARQADRELLAQAPAGFLDTTHFDTVRFPPPP
                     WALRAFAEAAADGSLAYTGYRGNARVLEQVAGSLAGFTGERFDPHRELILTPGTQAGL
                     FGALSALVNEGDLVLLVDPDYLFSERILRFLGARVQSVPLLTEGEIRPDLDLLEECLR
                     QQQPKLLLFSHPNNPTGAVYSPAVIARLAELAVRHDLWVVVDELYARLLYDDVSYAHL
                     AAQPSMRERTVTLFGPSKTESLSGYRLGVVAAPPQVIAAMEDVQSITSLRAPAYAQHV
                     LSPWLRDDRDWLAERLQEFDRLRHMTVEAFRSMPWLRISLPAGTAYAWPDVSALGQPD
                     TVIAGALLKEAGVLVSPGYQFGAHGCNHFRVCFARDEREWAAALERMKSVLEGFARKN
                     GLTRG"
     gene            20572..21564
                     /locus_tag="VFB37_00315"
     CDS             20572..21564
                     /locus_tag="VFB37_00315"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZO20911.1"
                     /translation="MAGQSLLRRVGNVNVLEHDGARAASLNCIRRLLTWMTAGLILGP
                     AGAAHAEVTVLDSTAVYPEGPLWHDGALLFVEYSANDIKLWDGHATKQYWKKAGCGPS
                     GLIGFGKNHLLVACYDANSIVELDAHSAEVRTLTTDRGGQPFHGPNDFTSDRHGGVYF
                     SASGAYDVAAPSTGAVLHLSASGADLKEVANKIHYSNGLTLSKDGKTLLVAEMLSQRI
                     LAFPVLAGGALGERHVWVSLQDLAPAPQDQGAYNGPDGFKLGPDGNYYIAQNGSGRIL
                     VVSEGRKLLKAISVATPYVTNVAFGKDDARTLYVTGTFDEWNPPYAGAVYRVVQ"
     gene            21609..>21988
                     /locus_tag="VFB37_00320"
     CDS             21609..>21988
                     /locus_tag="VFB37_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HZO20912.1"
                     /translation="MHVNTKCARAVAAILGAHAAAAVHAAAPADETAASGGGLEEVIV
                     TAQRRSENAQDVPIAIQALTGETLQQLNISNFDDAVRYLPNVAGSSAGPGQTQIFMRG
                     LSVGNQGTQSGGSINGFPNVAIYL"
BASE COUNT         4059 a         6950 c         7110 g         3869 t
ORIGIN      
        1 ggtccgcagc cggcacgggt ccgcagccgg cacgggtccg cagccggcac gggtccgcag
       61 ccggcacggg tccccaggcg gcagattttc gcccgaattg tccgattcca ggaggctcga
      121 tcgtcttcaa ggcaggaaac attgaagcgg aagatgaccc atcatgacct tcgcctcagc
      181 aaccccagcc agccggcaga acatgctcgg ggacgttgta ggctctggct gggtcacgcg
      241 ctgtggaaca ctgttgcggc agctcggacc ctacgccgcc atcgagttgc tcctgcctgg
      301 tggctctctc atcgtgctgg ctctgtggct gtatcggcgc catcgtctcg gccggcgcga
      361 ggacgcacag tcaaaaatga tcgcacgcgt tgcttgcgcc attctgaccg ccgtgacggc
      421 gccgctcagc tttcgggctg tcgctgacgc ggcggttcaa tgcccgccgc cgcgattggc
      481 agttcaggca cgagcgtata ctctagcgta gcgttgccgc ttcgatcaaa gactctggga
      541 gaggcccaag agtttcggct gggagagagt cctgccgcag gccgcccggc aggcgcccga
      601 gttgctacag tggtgcgccc tcaacgacga aggcggatga ccgcccacag caaagcccat
      661 gttgaagctg ctgccgtact ttcaagcaaa cccactcgtg tcgattctgg ggttgctcgt
      721 attcgtgctg ctgctgcatt tcctcgtgca gtacatctcg cgcgggctcg ctctgcgcgc
      781 gcaactgatc cggctcgttg aggagatcgg cacttcaggg gcgcctggct cagcggagat
      841 gaagtcgcaa ctggcacagc tcttcaggaa aacccgcctg gcacgcgcgt ggcgtgagtt
      901 cgaggagtcg ctgcatgtgc aggacgcgga agtgggtgcg gggccgagcg cagcgcccat
      961 ccgctcgact cagtctgccg aagtgttctt caatctggaa accgtgatcg atccctggat
     1021 cggctcggag tatttccgcc atctgccggg catcctcacg ggtctcggca tcatcggcac
     1081 cttttttggc ctcatccagg gactcgtgca cttcgatccc agtctcagtg actcggccgg
     1141 tctcagaaga ggtctgggcg agctcttcgg gcacgtccgc gatgcgttca tgttctccgg
     1201 ttcggcaatc gcagccgcca ttgccgtcac gggggcggag aaatggttgt acgcatcctg
     1261 cgccaaatgg gtcttcgaac tggcggcggc gctggacgcg cagttcaaga gcggtgtcgg
     1321 tgaggaatac ctggcctcga ttctcagagc ctctcaggac agcgccgtac aggtgcggaa
     1381 tctgaagacg gtgatggccg aggaccagcg ctcgctcctg acgtacctgg cggaccggca
     1441 gctacaggcc acgcagcaga tggcggcgga gatcggacgc agcatccggc aaagcctgca
     1501 ggagccgctc gccgacattg cccgaaccgt ggcgctggtc agtgggcggg aaaccgagca
     1561 cctgaaaacg gcgctcgaac aactgatgac ttcgttcctg gagcagatgc gcgagaccat
     1621 ggggcgccag atgggcgatc tcagcagcct cctgcagcag tcggcgcgtg cggtccacca
     1681 gctcgagatc gccatgcagg acttgctgca ggatgtgcag cagagcggtc acgactcggt
     1741 agcgagcatg cggacggcgg tccaggagct ggtgcagaaa ctgtcggaaa ctcagcgggc
     1801 gcagagtgat ctggtctcct cggcgatgaa ggacgtcctc gggcagctca aggaggcgac
     1861 ggtgcgcatg gcccttgcgc aggaggaggc cgcgcggcgg gcgcgtgagg gaaaccaggc
     1921 tgcggaagct catatgcgcc ggcaggttgc gacccttgcg gattcccata tgaccacgat
     1981 cggagccacg cgggagctgc tggagcgctt caacggctct tcggcggcga tgatcgacaa
     2041 gctgagcctg ggtaccgtgt cgctggtgag tgcgatgaag tccgtgcagc agtccgccga
     2101 acagctgtcg cgggtcggcc tcgagctcgc cgccctggaa ggacaggccc agaggtcttc
     2161 gcaggacatg gtgcgtgcca gctcgcacct ggcgcttgcg gcgcagaatg tcggcaactc
     2221 tatccatcag ctgagcaatg ccgcggtgcg cttcgagggg gtcgcttcga gtgcgggcgt
     2281 ggaagccaac gcccgccggc agattctggt cagcctgcag gacctcatcg accagtctca
     2341 gattgcgagc cgcgagttcg tcaacctggc gcatgaagcg cgcaaggcac tcgatgcggg
     2401 tgtggagcgt ttcgactcgg aggtgagtac cgtattgtcc ggtcatgtca ggacttatca
     2461 gaagcagctc ggtgactcgg tcagcgggtt gcgccgcgcg ctcgagcaac ttgcggcgcg
     2521 tacccgggat cgcacgtgag cgtactgcag cagctcatcc ggcccgcgcg gcggcggccg
     2581 gaggccgagc gacctttctg gatctcttat gcggatctga tgacggccat gatgacgctg
     2641 tgcctggtcg cgatggcggt caccatcatt gccatgagtc aggagctgcg gcgcgagctc
     2701 agtgccgagg atacccgcgc gcaggagatc ctcggcatct gcaatgagat caagcagggc
     2761 atccgggacc ggccgaacgt gcatgtggac tgccgcgaca accggatcga tttcggggag
     2821 gcgggtcagt tcggttacga ggaataccgg ctgcacccgg aggtcgaaag cgacctggcc
     2881 gcgctggtgc ccgtgatcct ccgggcctcg aatgatcggg atgggcgcaa atggttcaag
     2941 caggtcgtga tcgaggggtt caccgacacg cgcgggccct atctctacaa tctctatctg
     3001 agcctgaagc gctcgtactg gatgatgtgc atgctggtgg accctcggcg taatgcctcg
     3061 ctcggcctca gcgatgagca ggtccagcag gtgcgggagc tatttctggc gggcggggta
     3121 tcgttcaacg atgccaagcc gtccgacgat gcgagtcggc gcgtggagtt gcgattgcag
     3181 ttcttcacgc gcaaggaaaa ggaagagccc gaccaggcgg cgcggcctca tttcatcacg
     3241 acacagcagg aaagctgcga gctgaactga ggcgcggctg ccgcccggac ggggagctgc
     3301 ccggggcggc caatgtaacg actgccgcca gtcccccgtc ggcacggttg tgcagatgca
     3361 acccgcctcc atgagcgatt gcgatccgct cgacgatggc caggccgagt ccagcaccag
     3421 tgtgcgagcc gcgtgaagga tcctcgcggg cgaatggcct gatcagcgca ttcgggtcca
     3481 cggaccggat tcccggccca cgatccaaca cagagaggac gatgcgctca tcagtgcgct
     3541 cgaggtgaat ctccacatcc tggccgccgt agcggatggc attatcgagg aggttggtga
     3601 tcaggcgccg catggcgagt gggcgaaaca tgaaggtggg gcccgggatc agctgcatgc
     3661 gaatgttccg gccgaccgcg cgatagcggc tgcagacttc tgccgccagc gcccccagat
     3721 cccccgcaac cggtggctcc tcgcgaccgg tcttggcgta gtcggtgaat tgctgcagga
     3781 tcgcgctcat ctcctccagg tcctgcagca ttccctggat atcggcgggg gatccccgca
     3841 tgtcgagcag ctcgagcgcg agccgcaggc gcgtcagggg tgttgcaagg tcatgtgaaa
     3901 tcccaaccag catcagacgg cgctccgact cgagcgcttc gaggttcagg gcaagctgat
     3961 tgaagccgcg gctgagctgg cggatttcgc tcggcccgcc gtccggtaac gcttcaggct
     4021 tttggccgct ccccatgacg cgcgcagcct ggaccacgct ggcgagttgc cggcgcagcc
     4081 agaagaagat gacgtagatt ccggctgctg aagtcaacaa agcaaacgcc gcccattcga
     4141 gttccacata aaggcgcagc accccagccg gatgcatgac ctgatcggcc tgcacgtagc
     4201 gattgaacac catcggtcgc acgacgctga accagagaat gtgattcgcc atgacaatca
     4261 gcgtcacgaa cacggcggtg cgcccgacca gcgatcttgg gatcaggctc atgcgggcga
     4321 tggatccggg acgaatacat agccaaagcc ccaaaccgtc tgaatgaagc gtggtttcga
     4381 ggagtcctcc tcgatcagtc gccgcagtcg ggagatctgc acgtccatgc tgcgatcgta
     4441 tgccccgtgc tcgcgtccgc gagcgatttc catcagcctt tcgcgtgaca gcggcttgtg
     4501 cggatgccgc accagggcct cgagcaccgc gaactcgccg gtcgtgatgg gcaaccgctc
     4561 accggatcgg gccaggcttc gggtaccgag attcagctcg cagggcccga agcggacggt
     4621 cccttcgtgc gcggatggcg cacccaccgg cacttctggc ctacggcgta aaactgcctt
     4681 gactcgcgcg acgagctcgc gtgggttgaa gggtttcggc aggtagtcgt ccgcaccgag
     4741 ctcgaggccc acgatgcgat cgatgtcatc accctttgcc gtgagcatga tgatggggac
     4801 ttccaccccc ttgccgcgca ggcgcctgca cagactcaag ccgtcctcgc cgggcagcat
     4861 cagatccaga accagcagat cgaccgcggc cgtctgcagg acgtgctcca gctgcgttcc
     4921 gtccggctcg acgctcacct ggaagcccat ctcctgcagg taggactgca ggagggatcg
     4981 cgtgcgccga tcgtcgtcga cgacgagtat ccgtgccgat acgggcttca taacggccgt
     5041 gagtgtaatt gacatcgtca accgctgccg cggcggaaat aatcatttac aaattgggcc
     5101 cggggagcaa caccccgttt acagctcccg gctatcgtgt gaacgctggc gccgagagcg
     5161 cttcgaggct tccagcaccc aatggcggct cgagcgcccc tgaaaccatc cggagcccgc
     5221 atgcgcggcc tgttctacgc gaccgttgtt ctcgcttccg ccggggtcac ggcctcaccg
     5281 gtcacctaca cgatcgatcc cgatcacacc catccggcat tcgaggccga tcatctcgat
     5341 ggtttatcgg tctggcgcgg aatttttcga acgactcatg gacggatcac gctggaccgg
     5401 gcggcccaat ccggctcggt gcagatcgaa gtcgaggttg cgagcgtcga cttcggaaac
     5461 gagcagctga acgagaccgc ggcccacgcc agcgcaccgc cgatcttcga agcgcagcga
     5521 tatcccacgg cccgatatga agggaaactc acgcaattcg tgcgcggggt cccgacccgg
     5581 gtgacgggta cgctcgagat gcacggagtc agccggccgc tgcaattgaa aatcgactcg
     5641 ttcaagtgcc tgcagcatcc gctgcgcaag cgggaggttt gcggagccga cgcaagtgga
     5701 acactgaacc gggcggattt tggcatcaag gtcggacaag gttatggatt ccggatgggc
     5761 gtgcggctgc acatacaggt cgaggcgatc cgggccggct gagtcatgcc tgcgctgacg
     5821 gacagccctg ctcggaaaca ggcaaggcgc agaccctcga gtcgcgtcct tcggggaatc
     5881 tccgctggtt tcgcagtgtt ggcggccctt gtgctcgcgt ggctcctgcg ggcgccgcgc
     5941 acctctgcca tttccgcgcc cgatgaggcc ctgccggtcg caacggccat agcgtttcaa
     6001 cagtccatgc ccgtgctcgt cgaagccgtc ggcgaggtca tctcgcgaca ttccgtgcag
     6061 attcgcccgc agatcaccgg gacactcagg cgcgtctgct tcagcgaagg ccaggaggtg
     6121 gtagcgggcc agcggctgtt cgaaatcgat cccgcggtct tcacggcagc cgccgcggcc
     6181 gcccgggccg cctgggagag cgcgagagcc aatgccgagc ggctgcctcc gctggcgaag
     6241 caggggtacg tcacgccgca ggatctgctc aatgcacgca gcgctacaga tcaggccgaa
     6301 gcagcctaca agcaggccgc aatcaatctg tcctacacgc aactgcgagc tcccatcgcg
     6361 ggccgtaccg gcgtgctcag ccgcaaagcc gggaacatcg tagcgccctc cgatgcggcg
     6421 cccctcgtga ccatcaacga gatccggccc atcgaggttc agttcagtat tccgcagcag
     6481 actctgggcg ctgtccgcca gcaactggcg gcacatgcca tcagcgtgcg aatcagctcc
     6541 gaagatggcg cggagactct ggacgagggc gttctcggct tcatcgacaa cacagtcaac
     6601 gcgcagaccg gcacgatact cctcaaggcc cgcactccca accgctcgga gcagctgtgg
     6661 ccgggagaat atgtgggggt gcacatggag ctgcgagtgg agccacgcgc catcgtcgta
     6721 ccggcgaccg ctgtgcagac agggcaggag ggcccgtatg ttttcctggt gacccagggc
     6781 gccgctgtgg cccgcaccgt ccgggtcgac cgggaagtcg gcgtgctcgc ggtcatcagt
     6841 tccggattga ctgccggcca acgggtcatt acgcgggtac cgcgaggcct gcgtgccggg
     6901 atgcgtgtcc ggccgcaaga cgtcgctgtc ggcagccggg cgacggtgac agtccccgaa
     6961 tcgccatgaa tatttccgtc gccttcatcc ggcgacccgt gatgaccacc gtcctgagtt
     7021 ttgcgctggt ggcttgtgga actgtggcct atctgcgcct gccggtgagc gagctgccga
     7081 acgtcgactt cccgacgatc agcgtctcgg cgagcttgcc gggcgcaaat ccgaaggaga
     7141 tggccgcgac agtggcgacc ccgctcgaac gccggttggg cggcatcgcc ggcctgatcg
     7201 cgatgagctc cgccagcacg acgggaaaga caacgatcgc gctgcaattc gcactgtctc
     7261 gcaacatcga tgcggcagcc gaggacgtgc agatcgcgat ctcgcagagc tcccgccagt
     7321 tgccgcccaa catgcccacg cctccgacgc tgagcaaggt caatccggcg gacaacgcca
     7381 tcgtgtatct ggccctgacg ggagatcggc tgccgctgac ggatctggat gaatacgcgg
     7441 agacgcagat cgcggaacag attgcagagg tgcccggggt cgcccaggtt caggtattcg
     7501 gatcctatca gtatgcgacc cggatctacc tcaatcccta tgccctgtcg gcgcgccacc
     7561 tgacgctcga catggtgagc gatgccatcc gcaacagcaa tacgagcttg ccctcgggca
     7621 cgctatacgg gggcacgaga acctatacgg tcgaatccaa cggtcagctg gcgaatgccc
     7681 aagcatacaa ccacatcgtc atagccgagc gggatggcgc gcctgtgcat ctgaacgatg
     7741 tcggcaaggc ggtcgatggt attcagcagg acaggcagct cacgacattc agcgacgcca
     7801 cgaccggtgg cgaactgcat ccggcagtca tgctcgcaat ccgtcgccag ccgggcgcca
     7861 acaccgttgc agtggcgcgt ggcatccagg agctgctgcc ccggctgacg cgtcaggccc
     7921 cgggagatgc aacgctgcga ctgctgtata cgcgggcgga tttcgttcgg gattccgtca
     7981 gggaggtgcg caacgccttg ctggctgcga tgggactggt gatcgcagtc gtgtttctct
     8041 tccttcgcag ggccggaacc actctcatca gcgcgttgag cctgccggtc tctctgctcg
     8101 gcacattcgt cgcgatgcga ctgctcggtt tcaacctgga tcacctttcg ctgatggggc
     8161 tgaccctggc cacgggcttc atcatcgacg atgcgatcgt ggttctggag aacatcgtgc
     8221 gcttgcgcga gcgcggggtg ccccagctcc tcgcggcaga gtcgggcacc gctgagatca
     8281 gctcgacggt ggtttccatg acgctctcgc tggtcgcagt gtttattccg atcctgctca
     8341 tgggtgggat gctgggtcgc ttgttccggg agtttgccgt gacggtcagt gcggccattc
     8401 tgctctcggg cctcgtgtct ttgactctga cgcccatgct ctgcgcggtg ttcggcgctg
     8461 caggtgcgga gcggctcagg accccgccgg gcatgagcag ggctcaggcc tggtaccgtc
     8521 gatccctcgt gcgttccctg gcgcattggc cttcgctgct gggcgccgca gccttcatgc
     8581 tggtgctcgc gatcggcttg ttcgcgtgga ttccaaaggg actgatgccc agtgaggata
     8641 ccggccagat aatcggtact acgctggctc ccgacggaac gaccttcgag cagctcaatg
     8701 acgtgcaaca gaacgttgcg cgaatcgtgc agcgcaatcc ggcagtcgcc acggtgatgt
     8761 ccaatgcggg ccagggctgg ggcagcacag gaggaaacaa cctcggactg ctctacatgg
     8821 ggctgaaacc cgtggcagac cggtcgagtg gggctgaggt gcgagcgcaa ttgcgccagg
     8881 cgctcgccca tgtccagggg ctggacgtat tcattgaaaa tccttccacc ctgaatctca
     8941 actcgatcaa cagcaatgcc gagttccagt acgtgctgca gagtcccggc atcgatgcgc
     9001 tctaccgcga ggcccccagg ttcgagcaac tgttgcgtca ggttcggggg ctgcgggatg
     9061 tcagcagtga cctatcgctc aacaatccgc agctcaaggt cgacctccgt cgcgaggcgg
     9121 cctccgctct cggtgtcacc gccgagcagg tgcaatcgac gctcgcggag gcttacgggg
     9181 gccagcaggt gagtacgatc tacggcccgg cgaacgagta ctgggtcatg atgcagctgg
     9241 ctccgcaata ccagacaaat ccgcgcgccc tggatgccct gtatgtcacc ggagcgggtc
     9301 aaaacctcgt accgctcagt gccgttgccg gcatctccgc tggggcgggg cccgtcagtg
     9361 tgagccacta cgggcagctt ccggcggtca ctctgtcgtt caacctcgat cccggtacat
     9421 cactcggggt ggcaaccgag cgaatcgaaa ccctcgctcg aaagtctctg agcgcgcagg
     9481 tcacggcaga ctttgccggg gccgccaaga gcttcacgga gtcaagccgt gagcttcttc
     9541 tgctccttgg cctctctgtt ctggtgatct atgtcgtgct ggcagtcctg tacgagcact
     9601 tcatccatcc gatcacgatt ttgacggccc tgccgctggc gatggtgggc ggactgctcg
     9661 ctctgctcgc ctgtggtcag gaattgaacg tcttcagctt cgtaggtttg gtcctgctga
     9721 tcggactcgt caaaaagaac ggaatcatca tggtggactt tgctctgcag atgcgccgtg
     9781 agcagggatt gagcgccagc gaggctatcg tagaagcctg cacggttcgc ttccgaccaa
     9841 tcatgatgac gactcttgcg gccatcctgg gcaccctgcc gctcgccctc ggctccgggg
     9901 cgggcatcga gacgcggcgg ccgcttggca ttgccgccgc gggcggactt ctcgtctccc
     9961 agttcctgac gctctacatc acgccggcgt tctacctggc gatggaaaat ctcggtgact
    10021 ggtaccggcg caactgccgc ggggatcgcg ctgacccagg gggccgtcac gcactgtgag
    10081 gcctcgccgg caggtgccgg attcgatggg cctgtagtgc attagctcac gcaacccctc
    10141 tcggcggccg catgcctcaa cggctgtaca gccgtggtag ggcgcgagct actcggcgga
    10201 gcggattcat cggaaccgag cgggcgaatg agcgttcctc ttgcgtccaa gccctcgagg
    10261 aatgcttcga tgaaccgcaa atccagactc gccgttgcgc tggcgtgggt aactgctgtg
    10321 ctgatgaccg tttcggcgca agcctcgcag agcgcccgac acgaagcggc cgaactgcac
    10381 cgggcaaagt tgtccttgat cgaggccatt gtcacggccg agaagcaggg cgctggccga
    10441 gcggtgagtg cctcgttcga gttcaagcga ggtaatccgg cttatttcga ggtcaaggtg
    10501 ctcagcccgg acggcaggaa actcacgggc tacaagctcg atccccgcac cgggcaggtg
    10561 caggatacga gtaacgaggc gctggaaaag ctgatctcgc ggatcactcc agagagtcta
    10621 tcccgagcgc ccacgacaat gactcatgcg atcgcccttg ctcaggagca ctcgggcggc
    10681 cacgcagtga gcgccgacgt ggatcacgat ggggatcaga tcaactatac ggtggagacc
    10741 gtcaaggccg acggcgcctc gcaaaagctc aaggtccagg gtgcggacgg caaggtgctc
    10801 tcatcggatt aatctgttcc gcagtcccgt cctacagccc ccaccagcgc agccgcttca
    10861 tcaggatgac ggcagaggct ggacagaggc tcacaaactc cggtgtcgcc cggattgcga
    10921 gcggacaaga ttcgcgggtc gtcggcccgt acccgagatc ctcaaagaac gcctgagcag
    10981 tcgtcgtcag cagatacacg gtgcggacgc ctttccctag cgcgtgtctt tcggcgtgct
    11041 gcacgagagc cgagcccatt cccgctcttc gcgactcggg tgcgacggcg agcgatcgca
    11101 gaagcgcatc gtgaccatga atctcgaggc ccaccaggcc cgtgggcgat tccgcaggac
    11161 cgcagaagag gaagtcagcg aggtgccgct cggccaggtc cgcggccggc aggccgacgg
    11221 agccgagaag agccgcggcc gaggcaagcg gcggtcctgg gcgcacttcg aattgattca
    11281 tgcgatggat gaactggaca tccgatctta caacatgggc gggggatgat gcgcaatcca
    11341 atgccgcgcc agggcctccc ggctgcacac ccagaccgcc tcgtgcgcct gaatgtggtc
    11401 cagcagtcgc gccagcgcgc cgaggcgtcc agggcggccg attatccgac cgtgcaggct
    11461 gagagtcatc atccgcgggc gcccctcctt acccagccgg taaagccact cgaaggcatc
    11521 ccgggcgtag gtgaagaact cctcgccgct ggcgaagtcg ccgccgcgct gcaggcgact
    11581 gtcattggtg tcgaaggcgt gcggcacgat cagatagggc tgcccggcaa cccgcatcca
    11641 atagggcaga tcgtcgttgt aggcatcact gtcgtagaga aatccgccgt gctcggcaac
    11701 caggcgccgg gtgttgggcc cgggacggcc ggtgtaccag cccagggggc gcctgccgat
    11761 cagccgcgtg atgatctcga cgttgtgctc gatgtgggac cgttcgaggt cctccgcgac
    11821 gaactggtag tcaatccatc gctgaccatg acaggccact tcgaagccgc tttgccgcat
    11881 ggcggcggcg gcctcggggt tgcgctccag agccatgccg actgcgtaga tcgtggcctg
    11941 cacgccccgc tcgtgcagca ctcgcatgat ctcccagaac ccaacacggc tgccatactc
    12001 gaagttggat tcgacattga ggttgcgcgc tccagagagc ggctcgagcg cgcccagatc
    12061 ggtcaggacc gactcggaat gctcgtcccc atgcagcacg cagctctctg caccttcctc
    12121 ataattgagc acgatcgaga ctgcgacgcg cgcctcgcct ggccagcgcg gatgcggcgg
    12181 atgggctcca tagccgatca ggtctcgagg gtaggcttcg tcccggctca tggctgcacc
    12241 tcgggctgag gcacggtccg ttcggccgcg atccaatcca tgtacgcgtg cagcgcctcg
    12301 cgcaccggcc ggggccgcca accggtctcg ctgatgatgc gcgagatatc gtaagccccc
    12361 cacatcccgc tgctcagggt agggtccacc acgatgtcgg ccgtctcgat cggctcgatc
    12421 cgggcgctga atccaggaga cttttccgca gcccagccga ccagatcagc cacgctgcag
    12481 gtgtttccag ctgcaatgtt atagacgctg tgtcgcagag ccggagcatg cagcagggca
    12541 atgatcgcat cggcgacgtc ctcggcatgg atgtagtccc cgaccgcttc gagcgagttc
    12601 accctgacac acttcacccc ctcgacggcc atatgggcga tgcggttggg aacgtgtcga
    12661 aagttcctgc tgctcgtgat gcgatccatc gagccgaaaa ccgatgacgg cctgacggac
    12721 agggtcgaca cgccgaacag gtcgccatat cgctcagtga tgagttccga ggcgagcttg
    12781 gagatgccgt agagcctgcg cggcatcaca tacccgtcct ccggcagcgg ctctccgggg
    12841 cgatcggggc cgtggtgttt gtaaaccgca cccgagctca cgtagatgaa tcggcgcagc
    12901 ttgggtagcg tcctcgccca atccagcact gccacggttc ccatgagatt cacatcgagg
    12961 atacggcggg gatcatccgc ctcgggctcc cgtcgagcct cggcagccgt gccgcgtgag
    13021 atcggggtga tcgtggcgcc gtggacgatc tccgtgatct cgcttcgcgc caggccctgc
    13081 cgccaagtgg cgggctgggt tacgtctgcg acgagcacgg agagcctctc ggagacgggc
    13141 tcgaagtagc gacgcgcggc ggcatccagc ggtgcggcat ccagaacaac ggcacgttgc
    13201 gcgcggccgc cgtccagcca gcgcctcgcc aggacgctca tcacgaagcc ggtgccgcct
    13261 gttatgagca atgtcacagc gttcgcgccg gcgatcagag ggggaaatcg agcaacagcc
    13321 ccagcagcgc ccgggtgccg accgcgaaat catcgagcgt catcgcctcg gcgggattgt
    13381 ggctgccttg ctcattgcgc acgaagatca tggcggttgg aatccgcagc tgggcaaaca
    13441 ctgcggcatc gtgtccggca ccgctggcca tctcgaaagg ctgttcgagc aaccccatga
    13501 gccgcgcccg caaccgagta tccatcaccg cagggggcga gtcactggtg gtgccgagct
    13561 cgaagcggac ccgataggcc tgctcgagcg ccaccgctgc ccgtcgtgcc tcatggatca
    13621 ccgcggccat cgtcgcatcc gcgagactgc gcacatcgat gacgaagctc acctcgcctg
    13681 cgatcttgct cggaccgtgc agcacggggt tggtgtagag ctcgccggat gtcatgacca
    13741 ggtcggcccc ctcggcctcc tgctgaagcc agaacgcctc cagatggtgg agcagtgcca
    13801 cggtggcggc aactgcatcg cgtcggtaca agcgcgagac ggcgcccgag tgagcgtact
    13861 ccccgacgca gcgggcattg cgcaatcgct tgcagcctcg tatgccagtg accacggccg
    13921 ccggaagccc tcggttgacg agggtcgggc cctgctcgat gtgcaactcc aggtacgcgc
    13981 gcaccgtttg aggatcaagc agcggacgac gctcgcgaat gggcgcagga tcgaaacccg
    14041 cctggcggat cgcctgctcg aggctgcagc cattgtcgca acgggggacg gcgaggcagg
    14101 tgggatcgag cagtcccagg gcgccggcgc tgccgatgta ggcgacgtcg aaccaggcgg
    14161 attcctcagc gcgaataccc atcaccacga ggtcgcaatc cggcacgaag gcatcctggc
    14221 gcagtgccga gaggacggca atcccggcta cgacgcccgc ggcaccatcg aagttgcctc
    14281 cctgagggac gctgtccaga tgcgagccaa tgagaatgcg tggtcggcta cgatcgcgtc
    14341 cgggtagcac catcatcagg ttgccgatcg catcgacgtt gacttcgagg ccgatctgct
    14401 cggccgccgc gcgcaggatg tcgtgcgcgg cctgctcgcc agggccgtac gtatcgcgca
    14461 cgatgcccgc cccccggcgg gtcgcccgat cgagtctctc aaagagcaat tgcgccagcg
    14521 caaggtcggc ttgtattgag cgtccgctca tccggcgagc catccgccat cgatcaccag
    14581 attcgacccg gtgatgaagc tcgcctggtc gctcgccagg aagagtgcac cgttggcgac
    14641 atcctgcgga cgcccgaatc gaggccaggg cgtgcgccgc cgtgcgtatt cgagcagcgc
    14701 ggggtccatg gccagcccgg gcttccccgt cacgatcttc ccaggtgaga tgcagttgca
    14761 cacgatcagg tccttggcgt agtccactgc gatctgccgc gtcaggtaga tggctcccgc
    14821 cttgctgact ccataggcgg tgtcgcgtgg tgagttgacg atgccctgct gcgaacccag
    14881 attgatcagg cgaccgcgga cctcgttgcg gggctcctgg gtgatcatct gttcgactgc
    14941 tcgcttgcaa ccgtagaaca tgccggtgag attcgctccc agcacctgat cccactgcgc
    15001 cgggctcgtc tcgagcaacg gtgtgccggt gtacgtggcg gcattattga ccatgatgtc
    15061 gagtcttccg tggcttgcga ccgtgcgggc gaccagcgaa tcgacatcct cccagcgacc
    15121 gacgtccgtt ttctcgaaca ccgccgcgcc gcccgcccgt ttgatcagct ctacagtcgg
    15181 gtcaccccct tcgacagcct gcgtggtcag atctgcaatc acgaccatcg cgccttccgc
    15241 tgcaaaggtg agggcgatgg cgcggccgat tccagagctc gcgccggtta caatcgccgt
    15301 cttgccggca agtaagctcg acatgggtgc agacccttcg tgatcgctct agcgattggg
    15361 gatggaatag atcttgccgt gatagggcga cttatcgagt tggtcgaggg cggtgacgaa
    15421 tacggtcttc tcatccgggc tgaaagccag attgggtacc ccaggggagg ggagcgcgag
    15481 cttgcgcagc aggtgtcccg aggtatcgac cacgaaaatg atgccggcca gaggcgcatg
    15541 cacgtcccgc ggattctgac cgatgtagat ctgacccttg gagtcgatct tgactccgtc
    15601 cggatagatg tgcacgacat tgttggtcat atcgtccaga ttcaggaaca ggcgccggtt
    15661 gctcagcgaa cggtccgcgg cgatatcaaa ctcgaccaga cggtggtctt cggtttccac
    15721 gacgtagaga atcctgccat ccttcgacac cgccagcccg ttcgcggagt gcagatcgac
    15781 ggcctgctgc gcgatcgcgc cgtcggcggc catgtagaag accttgccat tgatgatggg
    15841 gcccggcgtt ccggagcagg tgaagtacag gccgccgtgc ccatcgggag caaagtcgtt
    15901 cggacctagg aacgcgttgc cagccttgtc gtgcgcatag ggcggaagcg gcttgccttc
    15961 cgccgacatg tggccgatcg tgccgttgtc atagcaggtc gtgaccagct cacctcgggc
    16021 ggtgggaacg accgcggagg gaccgcaacc cttctcggac cagaacacgg cgttcttgct
    16081 gccatcccag gtcgtcaccg tgttgcggtc gtactcgacg tagtaaagct tgtcgtggta
    16141 ccagatcggg ccttccggaa aatgcgcatc gctgttgagc acgcggatgc cttcagcctg
    16201 agctcccgag acgatgaagg ctgcggtgag cagccagaag cgtggatgat tcaaggcgtc
    16261 tcctcgacag ttttcatgca ctggagtatc cgatggcgcg ggcctgatcg gccgtcacgg
    16321 tacgggctgc caggatgaag tgccaggtag cccagaagcc ggtcggtgcg agcagcagca
    16381 gcgccagccg cagcgattca gcatcggccg cctgcccgtg cgcgaaccaa tcgctcagca
    16441 ggccaaccgc ctggggcgca acgatcaggt tgaacaggtt gcctacgagc acggaccagg
    16501 cggtgaaaat cgagcgcatg ttgctcggag cacaattgag caccagcgcc atgcaggggc
    16561 cgatgaagaa gtagatcgcg ggaatgaaca gccacagcat cagtctcgcc accgacagcg
    16621 agtgcgtcca gtaggtgagg aacgacggaa tcgtggcgaa gccaatcacc agcgcgaggg
    16681 tccatagcac gcggcgcgga tcaaccatgt aggggcgcga catgagccac accgttgcca
    16741 gcatcgcgct gatgccaccc acgaggtgaa tgttgccggt gagggcaccg gcctgagcca
    16801 cattcagtcc gtaacttcgc agcaggaaag tcggcgtcca ccacgcgagg ccccaacccc
    16861 agagcgagca cactccactc gccatgatca cgtgaaatgc agctttctgc cgccacagaa
    16921 atttcagcga gaccaggagt gaaggcttgc cctcgccggc gatcacatcg agccggccgc
    16981 gctggggctc gcggatgctc aaaaacacga cgatgccgag gatgacaccc ggaactccca
    17041 gcgcaaagaa ggcggcgcgc cagccgtacg cctgcgcaac cgccccggcg agattggccc
    17101 cgagccaggc gccaatcggt gctccgagag ccaacaccga cagggccatg ggacgacgtg
    17161 ctgccggaaa gtagtccgag acgatcgctg tggacggtgg cgtggcaccg gattcgccga
    17221 ccccgacgcc gatcctggcg agcagtagtt gccagtaagt ctgcgacagt ccgcagagca
    17281 cggtgaaggc cgaccagata accagtgagg ccgccacgat gttgcggcgg ttggatcggt
    17341 cggcgatcca ggcgatcgga ataccgaacg ctacatagaa caacgccagc ggaacacccg
    17401 tcagaaaggc cgcgccggca tcggtcaact tcaactcgag gcggattggc tcatagacgg
    17461 tcgtgacgac atagcgggcc gcgatattga tcgcgtacac catgcacatc acgatgagga
    17521 cgtaccagcg ctcgatgatg gatggtgtat ccgagcgggg cggtgcgatt gcattgacgc
    17581 tcaaactgaa tccccgcgga cgtcgggacg gctgatgcgc cccggtaaat gactgtaccg
    17641 cattcgatcc gagaggggga aggctcgccg tgctgcggcc ggtcagtctg tgctccggtg
    17701 ttcccgcgcg ccggggcaga aagatcagcg atggcaaagc ggttggttat acgataaggc
    17761 cattccacga ggtcaaagcg cgagcgtcag gacctgtaaa ctcaatggcc agtgactttt
    17821 ccacctcggc agcctctcgg cgtgccctga tcttcttcga tcaagccgat atttttctgc
    17881 cgcgtctcat cgagcgcttt ccggccatcg agttttctgc ctgcggcgca cacgacgagc
    17941 tcccggggat gctgaaccgg atccggcctg agatcatcct ggccttcaaa ttcgaggggc
    18001 ggcccttccc gcgtcgcgag ctcctcgctt gcgaggcatt gcgctggctg agcctcgcat
    18061 tcgccggcgt cgatgtgctc atgccctggg atgatgcccg gctgaccgtt accaacgcgg
    18121 cgggcgtggc ggcggaggag atggctcgct atgccttcgc tgcgatactc gggttgttcc
    18181 agggctttcc cgagcttcat gcccggcagg tgaagaaaag ctggcagttc cattcgcacc
    18241 ggtcggcccg cgatgcgacg ctcggactgg tgggactcgg gcacagcggg cgcgcgatag
    18301 ccaggatggc ccgcgctatc ggtctgcggg tcttcgcctg tcgaaattcc gcgcagccgt
    18361 ccgaggaagt ggatgcggtc tttccgaggg agcaactgca cgaaatgctc ggcagggtcg
    18421 atgtcgccct gctgtgcgct ccgctgaccc cattgacgcg cgatctcttc ggagcggctg
    18481 ctttcgcggc catgaagccc ggctcattct tcatcaacat ggcccggggc gccatggtgg
    18541 aggaatcggc cctgatcgag gcgctcctca cgggtcatct cgccggcgcg gtgctcgatg
    18601 tgacccggac cgagcccttg cccgcatccg acccgctctg ggacgctccg aatctgctga
    18661 ttactcctca ctcatccagt gaatacgacg gttggatcag cgatgcggcg ctgatgtttg
    18721 ccgacaacct ggaccgatgg atacatgcgc gcccgctgca gaacagggtc caccccgatc
    18781 gcggctatta agcgctgtcg cagcggtggg ggcgcctgcg cgggttttcc cctgaacctc
    18841 ctcaaccgcg ggtgagcccg ttcttgcggg cgaagccctc cagaacgctc ttcatccgct
    18901 cgagcgctgc ggcccactca cgctcgtcgc gcgcgaagca gacgcggaag tgattgcagc
    18961 cgtgcgcacc gaactgatac ccggggctca ccagtactcc ggcctccttc aacagcgcgc
    19021 cggcgatcac tgtatccggc tggccgagcg cgctcacgtc tggccacgca taggcggtcc
    19081 ctgcaggcag cgagatccgc aaccagggca tcgaacggaa tgcttccacg gtcatgtggc
    19141 gtaatcgatc gaactcctgc aagcgctcgg cgagccaatc ccggtcgtcg cgaagccacg
    19201 gggacagtac gtgctgggca taggccggag cccgtagcga ggtaatcgat tgcacatcct
    19261 ccatcgcagc gatgacttgc ggcggcgcgg cgaccacccc gagacggtac ccgctcagag
    19321 actcggtctt gctcgggccg aacagcgtca cggtgcgttc gcgcatcgag ggctgcgcgg
    19381 ccaggtgagc atagctcaca tcgtcgtaga gcagccgcgc atacagctcg tccaccacca
    19441 cccacaggtc gtggcgcact gcgagctctg cgagccgtgc gatgacggcg ggtgaataga
    19501 ccgctcccgt ggggttgttg ggatgcgaga agagcagcag cttgggttgc tgttgccgca
    19561 ggcactcctc gagtagatcc aaatccgggc ggatctcccc ctcggtgagc aagggtacgc
    19621 tttggactcg agcgccgaga aaccgcagga tgcgctcgct gaacagataa tccggatcga
    19681 ccagcagaac gagatcgcct tcgttcacca gcgctgagag cgccccgaag agccctgcct
    19741 gggtgccggg ggtgagaatc agctcgcggt gaggatcgaa gcgctcgccg gtgaatcctg
    19801 ccagggaccc agcgacctgc tcgagaacgc gcgcgttgcc tcgataacct gtataggcca
    19861 gagagccatc ggcggcggcc tcggcgaagg ctcgcaacgc ccacggcggc ggcgggaatc
    19921 gcacggtatc gaaatgagtg gtgtcgagga atcccgccgg ggcctgggcg agcaactcgc
    19981 gatcggcctg cctggcccca cccgccagcc gctgcggcaa acccagttca gtcacacgcc
    20041 tggaagggca gaacaggctg gttcggttgt ttggcatagc tcgaatcgtg gcgccgctgc
    20101 gaattcgggt cgatcgcgcg ggctgcaggc cgcgcatgga ccgaagcata agccaagaag
    20161 cagtgctgcg gggatggccc aggcaaccgc aggtaaatca aaaagttgtg gtgccggccg
    20221 ccgtggcgtg tggcgagcgc ggtattcgta cacaaacatc gtcatctacc gcaatggctg
    20281 atggcgctgt tgctcctacc cgacgcgtac gtgtctgcgc aggatctcga gcgcgctcgg
    20341 aaggcgcgca gatctcaaag caactccact cgtgggtatt gaaccacaga agtgttcccg
    20401 aaaatttgtt cgcagcgggc agcgatgcga atggaccgca cgtccctgcg cggctacact
    20461 gcaccggcga gtctccacga gaatgcggac caacacgcca gggcgatcac cgtccataga
    20521 gcctcgcgcc gtgaactcag gggtccgtcg gagaatcggg gtcgagcgcg catggctggc
    20581 cagagtctgc tcaggcgagt tgggaatgta aacgtcctgg aacacgacgg cgctcgcgca
    20641 gcctcgctga actgcatccg ccgcttgctg acctggatga ccgcaggact catcctcggg
    20701 cctgccggcg ctgcgcacgc ggaggtgacc gtcctggaca gcaccgcggt gtatccggaa
    20761 ggcccgctgt ggcacgacgg cgcgctcctg tttgtcgagt actcggccaa cgacatcaag
    20821 ctctgggatg gccacgcgac caagcagtac tggaaaaagg ccggctgtgg acccagtggc
    20881 ctgatcgggt tcggcaagaa tcacctgctg gtcgcctgct acgatgccaa ctcgatcgtc
    20941 gagctcgatg cgcacagtgc ggaggtccgt acgctcacca cggatcgggg cggccagccc
    21001 ttccatggtc cgaacgactt tacctcggac cggcacggtg gggtctattt ctccgcctcg
    21061 ggagcctacg acgtcgccgc tcccagcacc ggcgccgtac tgcatttgtc cgcgagcggc
    21121 gccgatctca aggaagtcgc caacaagatc cactattcca acggcctgac gctttcaaag
    21181 gatggcaaga ctctgctggt cgcagaaatg ctgtcacagc gcattctcgc cttcccggtg
    21241 ctcgctggtg gagccctggg cgagcggcat gtctgggtga gcctgcagga tcttgcaccg
    21301 gcgccacagg atcagggtgc ctacaacgga ccggatggat tcaagctggg tcccgatggc
    21361 aattactaca tcgcacagaa cggctcgggt cggatactcg tcgtgagcga gggccgcaag
    21421 ctcctgaagg cgatcagcgt cgcgacgccg tatgtgacga acgtcgcctt cgggaaggat
    21481 gatgcacgaa cactttacgt cacaggcacc ttcgatgagt ggaacccgcc gtatgcgggt
    21541 gccgtatatc gggtcgtgca gtgattgcag cggagtctgc ggaataacca caacgggaga
    21601 ctcggccgat gcatgtcaac accaagtgcg cccgcgcagt cgccgcaatt ctcggggccc
    21661 acgcagccgc cgcggtccat gccgctgcgc ctgctgatga gacggccgct tccggcggcg
    21721 gtctggagga agtcattgtc accgcgcagc ggcgcagcga gaacgcgcag gatgtgccga
    21781 tcgccatcca ggcgcttacc ggcgagacgc tgcagcagct gaatatctcg aacttcgacg
    21841 acgcggtgcg atatctgccc aacgtcgcgg gctcctccgc cgggccaggt cagacgcaaa
    21901 tcttcatgcg gggcttgagt gtcggcaacc agggaaccca gtcgggtggg tccatcaacg
    21961 gcttcccgaa tgtcgcgatc tatctgga
//
LOCUS       DASRHN010000009        15685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1651748, whole genome shotgun sequence.
ACCESSION   DASRHN010000009 DASRHN010000000
VERSION     DASRHN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15685
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651748"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1171
                     /locus_tag="VFB37_00325"
     CDS             155..1171
                     /locus_tag="VFB37_00325"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZO20913.1"
                     /translation="MVRIVRATQVEIETAVQTLRDGELVAFPTETVYGLGASAQNPAA
                     VQKIFAAKGRPTSHPVIVHLDSPRFLPRWVREVPEIANKLAERFWPGPLTMVMPRAFN
                     VHDVVTGGQDTIAIRIPSHPMAQQLLTAFGGGIAAPSANRFGRLSPTRPEHVRDELGD
                     AVKVILDGGECQIGLESTIVAIEGTNVRLLRPGAVTAGQLRAVLGELLIGASHDSPRV
                     PGSSPAHYSPSTPMTIIPAGEIDAHAAALSEGGRRVAVLAQRLPLRAHKYVTWVNAGR
                     RPEQYGHDLYANLRTLDKAGCVQILVQDVPEGDRWDAIRDRLLRAASNVSESDDTGAM
                     AVLP"
     gene            complement(1173..2681)
                     /locus_tag="VFB37_00330"
     CDS             complement(1173..2681)
                     /locus_tag="VFB37_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO20914.1"
                     /translation="MSPPSAPQDPRRELETLLASRFALIVIESREEARVLTLVRDASL
                     KVRHGRGWGVFQWTVTEGLRRLDVDMGGPQKMLSDPGQLLRHLKATPMAGIYVLLDFH
                     PYLSDPVNVRMLKDVAQGYDQVARTVVLMSYEVTLPQELESFAARLHLALPSSGERQM
                     IIKRVAQEWAAANPGHPAQIEPEALTRLADNLSGLTGADAERVVRQVIFAHGALTMSD
                     MQSVLAAKYQLLNRGGTLTYEPDTARFADVGGLQHLRAWLTSRKAAFDGSAPELDAPK
                     GVLLLGVQGCGKSLAARCAAGVFGVPLVRLDFGALYSKWHGESEKNLRESLQAAEALA
                     PCVLWIDEIEKALADGEGDSGTSRRVLGAFLTWLAEQRARIFVMATANDITALPPELI
                     RKGRFDEIFFVDLPNEPARAAILAIHAHKRSVPLSPQEVLALAHGSKGFSGAELEQAI
                     VAALYDARSSGARVDAAAIARELQATKPLSVVMAEKIAALRAWARERTVPAD"
     gene            2753..3931
                     /locus_tag="VFB37_00335"
     CDS             2753..3931
                     /locus_tag="VFB37_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HZO20915.1"
                     /translation="MRRRHRPRFIDPFQPTQIRRLVKEFGSPLLILDSERVRVQFRKL
                     RKALPGVDLHYALKPLPHPAVVQTVIREGGFLDLATTGEVQLVQRLGIGPERCIHTHP
                     IKRDQDIRNAVAFGMRTFVADNPDEVRKFARYAGSADLLLRVSFRSPGAVCDLSRKFG
                     CDPEDLLPLARLTAELGIEVRGLSFHVGSQAADSAKHVEALHACVKLMAAARREKLGA
                     LDTLDIGGGFPIDYAQPVQDISRFCAPLRSALARLPERVRIIAEPGRYIVGPSAIGVA
                     SVMGRARREGHWWYYLDDGLYGSYSGQLYDHARYPVEPLRDSPERLPSVLAGPTCDSI
                     DVIAENLMLPELRMGDLIVGRAMGAYTWASASDFNFFARATVVAVNTEPGDSGSVSAP
                     "
     gene            3976..4470
                     /locus_tag="VFB37_00340"
     CDS             3976..4470
                     /locus_tag="VFB37_00340"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HZO20916.1"
                     /translation="MTSFVSAPRVLLATEALRHLELTNVHTGEVVKVSFRDARSIAPD
                     ALARLQHLLRDYRRDEEHVMDPALYVLLSDLAREAGCEPRYEVISGYRAPATNEHLRN
                     TGHAVAEHSQHMEGRAMDVRLQNFPLSRLRDLALDARRGGVGYYPRSGFVHLDTGRVR
                     SWQE"
     gene            4515..5018
                     /locus_tag="VFB37_00345"
     CDS             4515..5018
                     /locus_tag="VFB37_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20917.1"
                     /translation="MKTDPAHEPDTVIHRQVAAAREGREPRVIARLYSGWALFGERQF
                     LRGYALLLPDPVVPTLNSLGAQERTLFLLDVARLGDALLKLTGAARINYAILGNQEPA
                     LHAHVIPRYGDEPESLRASHPWAYDWSRAPAFDPAACAELARGLHQELTRMGVTKRMR
                     FAPGTNT"
     gene            complement(5058..5894)
                     /locus_tag="VFB37_00350"
     CDS             complement(5058..5894)
                     /locus_tag="VFB37_00350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZO20918.1"
                     /translation="MNAAVGRRKAVCIAAGMLACATAAWAQDTPQNKPAPSSAPAPAA
                     AQPKTPASHKGATGAGVKTPTNMKAQGSYSLGVSMGTQLHEAGLTNESVATERLSQGL
                     RDALSGKATMSPQDQQNIQTFVKSVREGLAETNRAAAKTFLADNGRKKDIVTTASGLQ
                     YHVINPGSGEGPKPTDEVTVNYRGTLLDGTEFDSSYKRGQPATFPVNGVIPGWREALV
                     LMKPGAKWQLWIPPNLAYDERSPPPIPPGSMLIFDVEMIGVKAPKAPAAAPLPQVPQP
                     PK"
     gene            6080..6784
                     /locus_tag="VFB37_00355"
     CDS             6080..6784
                     /locus_tag="VFB37_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO20919.1"
                     /translation="MLRIRELCRSFHEGERVHRVLEHAEAEFRAGEVAAIIGRSGSGK
                     STLLNLISGIDRPDRGSVHIDSLEVSALAEPARTLYRRKHIGFVYQFFNLIPTLNVEE
                     NVRLALELNGVRGSEARTRSAEMLREVGLGERLGSAVDRLSGGEQQRVAIARALVHRP
                     ALLLADEPTGNLDDETSQEVLPALLSMARARGATLLIVTHDNALARLADRVWELRDGR
                     LQEISCQHSGQQASAI"
     gene            6748..9267
                     /locus_tag="VFB37_00360"
     CDS             6748..9267
                     /locus_tag="VFB37_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZO20920.1"
                     /translation="MSTLWAASFRHLMRHPAQLALALVGLSVGVATLVAVDIATASAA
                     RAFELSMRAVNGAATHEILGGPAGLDEQLYVGLRLHDPEAELAPLVEGYVTVGDRTMQ
                     LVGIDPFANPQFEPPGSQSQGPSAALDLAAATKWFTQQGSVLMAANTARELGIGQDQS
                     FSLQVGGHQQRATLIGLANAAPGYESLIMTDIAQAQEWLGLRGQLSRIDVKLPPGEAA
                     TDRLRSQLPPGVQLEPAQGRTRQSIDMTAAFTTNLKAMSLLALLVSLLLIYGTVSFAV
                     VQRRRAVGILRALGATAGDILTVVLTEAAGLGLLGAGLGLLLGVSIAHLLVRLVSRTI
                     NDLYFAVSVNQTVLPASAVAIALLAGFLTALVGALVPALEAAGGAPQLGLRRSVLESR
                     AVAIAGRLALASLALAIGSATLAASSGRSLLAGFVALFMLLLSVAALTPALLRAGAQA
                     AAKVCARVSPVARLAFGDIAASLSRTGVAVAALGMAIAAMIGVSIMVESFRESLREWL
                     QQTIRADIYVTAPGAGGPERRIDPQLVAQLLGTPGIADYSVSRHASLEVRGENVALDA
                     LQPAASSRPGLVFVQGSAQTAWSGLATGAILVSEPLAWRMSLQAGQDLTLPTLSGPRN
                     FRIAGVYREYGNDRGNLMMSLSEYRRLWHDDSITGLGIYLAAHTSPEQAIARLRAKAQ
                     GKQALLISSNTRLRALSMDIFERTFVITRVLYWLAAGVAAVGLLSSLLAWELERSREV
                     GLLRALGMTPRGAAVLIEVQAGFMGLMALLAAIPAGLLTALMLIRVINRRAFGWQIDL
                     HLTAAQFGNALLLAVAAALAAGLYPAWRSARAPIADEIRDE"
     gene            9321..10421
                     /locus_tag="VFB37_00365"
     CDS             9321..10421
                     /locus_tag="VFB37_00365"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HZO20921.1"
                     /translation="MLGMLAAQIMAAAGSLSELDVLRGVRAKSGFELALAPREFHFPL
                     DHGPHPTFRHEWWYVTGNLDAAGGARFGFELTFFRFALAPGNGTDTPARSQQSRWRAR
                     QIYLAHFAVTDVELGVFEFAQKYSREALGLAGAQAEPFRVWLDDWTLGGGGGSWNLSA
                     RGQGYELSLDAQLLGAPVLNGQAGLSRKSDEPGAASYYYSVPRMAVTGRLVRGGHPMQ
                     VHGLAWLDREWGSGALGPHQQGWDWFALQLHNGGTLMFYALRDGNGARDPHSAGTWVS
                     PTGEVRAIASEDVQIEVLDHWVSPRGGRYPSRWRMRVPVVGIDVEIRPVLANQELVTR
                     PRYWEGAVDVSSAQGGSAASGRGYVELVGYGE"
     gene            10431..11033
                     /locus_tag="VFB37_00370"
     CDS             10431..11033
                     /locus_tag="VFB37_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HZO20922.1"
                     /translation="MDYSRLIQRVRALLVSPRTEWPVIAAEPVTIADLYRDPIAVLAA
                     IGPVCAFIKTSLIGYGWHGFRVYRLGIGAGLAAAIVSYAVTLVAVYVLAVVIDLLAPS
                     FGGQQNRLQALKVAAWSCTGVWLAGFAHLLPSFSVLLFLAGDAYSVYLLYTGLPALMQ
                     VPPERSAAYTAAAAITALVLGWIVSLLTGGITGVRFGLDY"
     gene            complement(11050..15477)
                     /locus_tag="VFB37_00375"
     CDS             complement(11050..15477)
                     /locus_tag="VFB37_00375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO20923.1"
                     /translation="MSVLSSFHPAVASWFSRTFDAPTPPQQQAWPAIQAGRNVLIAAP
                     TGSGKTLAAFLAALDGLIREGLQNALSDETRIVYVSPLKALSNDIRRNLEAPLAGIRE
                     ELVALGLPEIAIRTFVRTGDTPQHERNSMRRHPPHIVVTTPESLYILLGSESGRKMLA
                     SIRTVIVDEIHAMVGNKRGVHLAVSLERLAALTGAPLTRIGLSATQKPIEDVARFLVG
                     MRHLRSDGTADCDIIDSGHVRARDLALEMPPSPLEAVMSGDLWTQVYDRLAELVNEHR
                     TTLVFVNTRRHAERVARHLSERVGETQVTAHHGSMAKELRLDAESRLKRGELKALVAT
                     ASLELGIDIGDVDLVCQLGSPRSIAALLQRVGRSGHSVGGTPKGRLFPLSRDDLVECG
                     ALLDAVRRAELDRLRMPAKPLDVLAQQIVAEVAALEWDEEALFNLIRRAYPYRDLTRE
                     EFDEIVRMLATGYSTRRGRHGALIYHDPVNHRLRGRRAARLTAITSGGTIPDAADYQV
                     ILEPQATFVGTVNEDFAVESLQGDIFQLGNVSYRILRVEQGKVRVEDAHGQPPSIPFW
                     LGEAPGRSDELSFAVSRLRQEIESLLPQGLDSAQRWLIDQVGVGGPAAAQLAGYLAAG
                     YGALGTLPTQQRIVFERFFDEAGGMQLVIHSPFGSRVNRAWGLALRKRFCRSFNFELQ
                     AAATEDTIILSLTTAHSFELADSARYLHSNTARAILVQALCAAPMFTARWRWCATVAL
                     ALPRFRGGKKVPAPLARMAAEDLLTSIFPDQLACAENLPGELEIPDHPLIRQTIRDCL
                     EEAMDIDGFEHLLRALERGEIQVVARDVTEPSPLALEVLSARPYAFLDDAPLEERRTQ
                     AVMSRRWLDPQSASDIGRLDPEAIQRVREEAWPDAANEDELHDALLWLTFLTQAEVDS
                     HAGWPELIQNLLLQRRITRLEICPSDAPKREQAAPPAQVLWLATERVPLFRAIFPDVE
                     PHPAIEVPAAYAQSLTREEALVETVRGRLEGLGPVTVTSLAHSVGVERGAIEAALAAL
                     QTEGFAMRGRFTSSAEPSAMEEEWCERRLLARIHRYTVKRLRAEVEPIQPRDFLRFLF
                     EWQRVTPEGRMEGPDAVAAVLGQLEGFEAPASAWETEILPARISEYEPAWLDEQCLAG
                     RFVWTRLAHRASGQRKNDSERGAAPVRATPIALLSRRNIRIWSSLSGPADERPLSARA
                     RLVADYIRSHGASFFDEIADGAGLLPAQAEEALAELVALGVVNSDSFGGLRALLVPSD
                     RRRPAVGGRRRRRISLFGMDAAGRWALVHRRPAAGPEQVDETASPALVQRRQDDETIE
                     HVTRVLLRRWGVVFWRLLAREADWLPPWRDLLMCLRRLEARGEIRGGRFVSGLTGEQF
                     AVPEAIGLLRDVRRAAHTQSYVSLSAADPLNLVGIVTPGARLPALSGNRVLYRDGAPI
                     AVFSGGEVRFLQELEPNERWQAHNLLLRRHVPAILAEFA"
     gene            complement(15474..>15685)
                     /locus_tag="VFB37_00380"
     CDS             complement(15474..>15685)
                     /locus_tag="VFB37_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20924.1"
                     /translation="RLGWRLDRLACLLSRVNQTLFRARTAGALERYCTQYELSRRPLP
                     ARGILWFNSDHPMKSFAVPCAAGRG"
BASE COUNT         2542 a         5127 c         5282 g         2734 t
ORIGIN      
        1 aagccgtata agctcgcgcg gcgcaccttg tcgtcgggag gcgacggaga gggatcgacg
       61 ccgatgtacg cgaagttgcc tctctttgac attgtctgag cctggattcg tgccgggcgc
      121 tcccactgcc ccgactggta gaattcgccg gatcatggtt cgaatcgtca gagccacgca
      181 ggtcgaaatc gaaaccgccg tgcagacact gcgcgatggt gagctcgtgg cctttccgac
      241 cgagacggtc tacgggctcg gcgcgagcgc gcagaatccc gcggccgtgc agaagatctt
      301 tgccgcgaag ggtcggccta cgagccatcc tgtcatcgtg cacctggaca gtccgcgctt
      361 tttgccgcgc tgggtgcgcg aggtgcccga gatcgccaac aagctcgccg agcggttctg
      421 gccgggtccc ctcaccatgg tcatgccccg cgcattcaac gttcacgatg tggtgacggg
      481 cggccaggat acgatcgcca tccggatccc ttcgcatccc atggcccagc agctcctgac
      541 ggcattcggc ggtggcatcg cagccccttc ggccaaccgc ttcgggcggc tttccccgac
      601 tcgacccgag cacgtgcgcg atgagctggg cgatgcggtg aaagtcattc tcgacggcgg
      661 cgaatgtcag atcggtctcg agtccaccat cgtggcgatc gaggggacga acgttcgact
      721 gctgcgcccg ggagcggtca ccgccggtca gttgcgtgcc gtgctgggcg agttgctgat
      781 cggcgccagc cacgactcgc cgcgcgtgcc cgggagctcg cccgcgcatt actcacccag
      841 tacgccgatg acgatcattc cagccggtga gattgatgcc catgcggcag ctctgtccga
      901 gggcggacgg cgggtagcgg tgctcgcgca gcgtctgccg ctgcgagctc acaagtatgt
      961 aacgtgggtc aacgcggggc gccgtcccga gcagtacggg cacgatctct acgcgaacct
     1021 gcgaacactc gacaaggccg gatgtgtgca gatcctcgtg caggacgtgc cggagggtga
     1081 ccgctgggac gccattcgcg accgcttgct gcgcgcggcc tcgaacgtca gtgaaagcga
     1141 cgacacggga gcgatggccg ttctgccgtg agttagtccg caggcaccgt ccgctcgcgc
     1201 gcccaggcgc gcagcgccgc gatcttctcc gccatcacga ccgacagggg cttcgtcgcc
     1261 tgcagctcac gcgcaatggc ggccgcatcg acacgagctc cgctcgagcg ggcatcgtac
     1321 agggccgcga cgatcgcctg ctccagctcg gcccctgaga atcctttgct gccgtgtgcg
     1381 agcgccagca cctcctgcgg gctcaagggc accgaacgct tgtgcgcgtg aatggcgagg
     1441 atggctgcgc gcgccggctc attggggaga tcgacgaaga agatctcatc gaagcgacct
     1501 ttgcggatca gctccggggg cagcgccgtg atgtcattgg ccgtggccat gacgaagatt
     1561 cgcgcgcgct gctcggccag ccatgtcagg aaggcgccca gcacccggcg ggaggtgccg
     1621 ctgtcgccct cgccgtcggc gagcgctttc tcgatctcat cgatccacag cacacacgga
     1681 gcgagggctt ctgcggcctg gagtgactcg cgcaggttct tctccgattc gccgtgccac
     1741 ttgctgtaga gcgcgccgaa gtccaggcgc acgagcggca caccgaatac tcctgcggca
     1801 cagcgtgcag ccaggctctt gccgcatccc tgcacgccca gcagcaggac gcccttgggc
     1861 gcatccagct ccggcgcact gccgtcgaat gcagctttgc ggctcgtcag ccaggctcgc
     1921 agatgctgca ggccgccgac atcggcgaaa cgagcggtgt ccggctcata tgtcagggtc
     1981 ccgccgcgat tgagcagttg atacttcgca gcgagcacac tctgcatgtc cgacatggtg
     2041 agtgcgccgt gcgcaaaaat gacctggcgc accacacgct cggcatcggc gcccgtcagg
     2101 ccgctcagat tgtcggcaag cctggtgagc gcctcgggct cgatttgcgc gggatgtccg
     2161 gggttggcgg cggcccactc ctgcgcgacg cgcttgatga tcatctggcg ctcgccgctc
     2221 gagggcagcg ccaggtgcag ccgcgccgca aagctctcca gctcctgggg caacgtgact
     2281 tcgtagctca tcagcacgac cgtgcgcgcc acctggtcat acccctgagc cacatccttc
     2341 agcatccgca cgttgaccgg atcactgagg tagggatgga agtcgagcag gacatagatg
     2401 cctgccatcg gagtggcctt gagatgccgc agcaactgtc ccggatcgga gagcatcttc
     2461 tgcgggccac ccatgtccac atccagccgc cgcaggcctt ccgtgaccgt ccactgaaac
     2521 acaccccaac cgcggccgtg ccgcactttg aggctcgcat cgcgcaccag cgtgagcacg
     2581 cgcgcctcct cgcgggactc gatgacgatg agggcgaagc gggaagcgag cagggtttcc
     2641 agctcgcgtc gcggatcctg aggcgcgctc gggggactca tacgtgtgcg gcaatgtagc
     2701 agaactgcga ccgcgccctt gtcagcccgt cacgtttttc taacaatgcg ccatgcgccg
     2761 ccgccatcgt ccccgcttca tcgatccgtt ccagcccacg cagattcgcc ggctggtgaa
     2821 ggagttcggc tcaccgctgc tgattctcga ctccgagcga gtccgggtgc agttccgcaa
     2881 gctgcgcaag gccttaccgg gagtcgatct gcactacgct ctgaagccac tgccccatcc
     2941 ggcggtcgtg cagacggtga ttcgcgaagg tggcttcctc gatctggcga cgacgggcga
     3001 ggtgcagctc gtgcagcgac tcgggatcgg gcccgagcgc tgcatccaca ctcacccgat
     3061 caagcgcgat caggacatcc gcaatgcggt cgctttcggc atgcgaacgt tcgtcgccga
     3121 taaccccgac gaggtgcgca agttcgcgcg ttacgccggg agtgcggacc tgctgttgcg
     3181 ggtctccttc cgcagtcccg gcgcggtgtg cgacctctcg cgcaagttcg gctgcgaccc
     3241 ggaggatctt ctgccgctcg cccggctgac cgccgagctc ggaatcgagg tccgcggcct
     3301 gtcatttcat gtcggctcgc aggcggcgga ttcggcgaag catgtcgagg cgctgcacgc
     3361 ctgtgtgaag ctcatggctg ccgcgcgccg ggagaagctc ggagcgctcg acactctcga
     3421 catcggcggg ggctttccca tcgattacgc ccaacccgtg caggacatca gccgcttctg
     3481 cgcaccgctg cgctcggctc tggccaggct gcccgagcgg gtccgcatca tcgctgagcc
     3541 gggtcgctac atcgtcggcc cctctgccat cggcgtcgcc tcggtgatgg gccgcgcccg
     3601 gcgcgaggga cactggtggt actacctcga tgatggtctc tacggctcct acagcggcca
     3661 gctctacgat cacgcgcgct atccggtcga gccgctgcgg gacagcccgg agcggctgcc
     3721 ctccgtgctc gccgggccca cgtgcgacag catcgatgta atcgccgaga acctcatgct
     3781 gcccgagctc aggatgggcg atctgatcgt cggccgcgcc atgggggcct acacctgggc
     3841 cagcgcctcg gacttcaact tcttcgcccg ggcgaccgtc gtggccgtca acaccgagcc
     3901 gggtgattca ggaagcgtga gcgcgccgtg agggggcggg gatggagccg gcgccgcgtc
     3961 ctgctcggcg ccctgatgac ctcgttcgtg tccgcgcccc gggtcctact cgcgaccgag
     4021 gcgttgcggc atctcgagct caccaatgtt cacaccggcg aggtagtaaa agtctccttc
     4081 agggatgcgc gatccattgc ccccgatgcg ctcgccagac tgcagcacct gttacgggac
     4141 tatcgccgtg acgaggagca tgtcatggac ccggcgctgt acgttttgct gagcgacctt
     4201 gcgcgcgagg ccggttgtga gcctcggtac gaggtcattt cgggctatcg cgctcccgcg
     4261 accaacgagc acctgcgaaa cacggggcac gctgtagccg agcacagcca gcacatggag
     4321 ggcagggcga tggacgtccg gttgcagaat ttcccgctgt cgcggcttcg agatctggcg
     4381 ctcgacgccc ggcgcggagg tgtcggatac tatccgcgct ccggtttcgt gcacctcgac
     4441 acggggcgag tgcgctcttg gcaggagtga cgtcacatct cgcgcacgac tgatgtgcta
     4501 ccttccacgc gtccatgaag accgacccgg ctcacgagcc cgacactgtg atccaccgcc
     4561 aggtggctgc cgcccgcgag gggcgagagc cgcgggtcat cgcgcggctg tactcaggct
     4621 gggcgctctt tggggagcgg cagttcctgc gcggctacgc gctgctgctg ccggatccgg
     4681 tcgtcccgac actgaattcg ctcggtgcgc aggaacggac gttgtttctg ctcgatgtcg
     4741 cgcggctcgg cgatgcgctg ctgaagctca ccggcgccgc ccgcatcaac tacgcgatac
     4801 tcggcaacca ggagccggcg ctgcacgcgc atgtcattcc gcgatacggg gatgagcccg
     4861 agagtctgcg tgcctcgcac ccctgggcct acgactggag ccgcgcaccg gcctttgatc
     4921 cggccgcctg cgctgagctg gccagaggcc tgcatcagga gctgacacgg atgggggtga
     4981 cgaagcggat gcgcttcgca cccggaacca acacctgagg agcggcgggc gttgagcccg
     5041 ccgcctgccg gggatgatta cttcggaggc tggggcactt gcggtaacgg agccgccgca
     5101 ggggccttcg gcgccttcac tccgatcatc tccacatcga agatgagcat cgagcccggc
     5161 gggatgggcg gcggtgagcg ctcatcgtag gccaggttcg gcggaatcca cagttgccat
     5221 ttggcgccgg gcttcatcag cacgagcgct tcacgccagc ccgggatgac accgttcacc
     5281 gggaaagtcg cgggctggcc gcgcttgtag gagctgtcga actcggtgcc atcgagcagt
     5341 gtgccgcgat agttcaccgt gacctcatcg gtgggtttcg gtccttcacc cgagccggga
     5401 ttgatgacgt ggtactgcag gccgctcgcc gtcgtcacga tgtccttctt gcggccattg
     5461 tcggccagaa aggtcttcgc agccgcccgg ttggtctcgg ccaggccttc gcgcaccgac
     5521 ttgacgaagg tctggatgtt ctgctggtcc tggggggaca tcgtcgcctt gcccgaaagc
     5581 gcatcgcgca gaccttgcga caaccgctcg gtggctaccg actcgttggt gaggccggcc
     5641 tcgtgcagct gcgtccccat cgaaactccc aggctgtagc tgccctgggc cttcatgttg
     5701 gttggagtct tcacgcccgc gcccgtcgcg cccttgtggc tggcaggggt cttcggctgc
     5761 gcggcagcgg gagctggagc ggatgagggg gcaggcttgt tctgcggcgt gtcctgggcc
     5821 cacgcggcgg tcgcacacgc gagcattccg gcagcgatgc aaacggcctt ccttcgacca
     5881 acggcagcat tcatgaggca cctcgggcat gcgggccgcg ccgagcaggc gcggcatgga
     5941 gccgcagcgg gcgacagttt ggcctatcgg cagggcggtt gctgcggttt ttttgccgcg
     6001 ctcgcgccgt atagaaggtg agaaagcgtg ggcggcgact tagagtagcc cgcgtgctga
     6061 gcaacccgga gcgggccgcg tgctgaggat tcgagaactg tgccgcagct ttcacgaggg
     6121 cgagcgggtg catcgggtgc tcgagcacgc cgaggccgaa ttccgcgcgg gcgaagtcgc
     6181 tgcgatcatc ggtcgaagcg gctcgggcaa gtctaccctg ctcaacctga tcagcggcat
     6241 cgaccggccc gaccgcggct cggtgcacat cgacagcctg gaagtgagtg cgctcgcaga
     6301 gcccgcgcgg acgctctacc gccgcaagca tatcggtttc gtctaccagt tcttcaacct
     6361 cattccaacg ctgaatgtcg aggagaacgt gcgcctcgcc ctcgagctca acggggtgcg
     6421 cggcagcgag gcgcgcacac gcagcgcgga gatgctccgg gaggttggtc tgggcgagcg
     6481 gctgggtagc gccgtggacc gcttgtccgg aggcgagcag cagcgcgtcg cgatcgctcg
     6541 ggcgctcgtt catcggcccg cgttgctcct ggcggatgag ccaaccggaa accttgacga
     6601 tgaaacctcg caggaggtgt tgcctgcgct gctgtcgatg gcgcgggccc gaggtgcgac
     6661 gctgctcatc gtgacgcacg acaacgctct ggcacgcctc gccgatcgcg tgtgggagct
     6721 gcgcgacggt cgtcttcagg agatctcatg tcaacactct gggcagcaag cttccgccat
     6781 ctgatgcgcc atccggcgca gcttgcactt gccctggtgg gcctgtccgt aggcgtcgcg
     6841 acgctggtcg cagtggatat agcgactgcc agcgccgcac gcgccttcga gctgtccatg
     6901 cgcgccgtga atggcgcggc gacccacgag atcctcggcg gcccagccgg gctcgatgag
     6961 caactctatg tcggattgcg cctgcacgat ccggaagccg agctcgctcc gctggtggaa
     7021 ggatatgtca cggttgggga tcggacgatg cagctggttg gcatcgaccc gttcgcaaac
     7081 ccgcaattcg agccgcccgg atcgcagtcg cagggccctt cggccgcgct cgatctcgcc
     7141 gcagcgacga aatggttcac gcagcagggc tcggtgctga tggcggcaaa cacggcccga
     7201 gagctgggta tcggtcagga tcagtccttt tcgctgcagg tgggtgggca tcagcagcga
     7261 gccactttga tcggactggc gaacgcggct cccggatacg aatcactgat tatgaccgac
     7321 attgcacagg cgcaggaatg gctcggattg cgtgggcagc tgtcgcgcat cgatgtgaag
     7381 ttaccccccg gagaggcggc aacagatcga ctgcgctctc agcttccgcc aggtgtgcag
     7441 ctcgagccgg cgcaaggcag aacccggcaa agcatcgaca tgacggcagc tttcaccacc
     7501 aacctcaagg ccatgagcct gctggcgctg ctggtcagcc tgctgctcat ctacggcact
     7561 gtcagctttg cggtcgtgca gcgccggcgt gccgtgggaa ttctgcgagc gcttggcgcc
     7621 acggcaggcg acattctgac cgtcgtgctg accgaagctg ccgggctggg cctgttgggt
     7681 gcagggctgg gactgctgct cggggtgagc attgcgcacc tgctcgtgag gctcgtgtcc
     7741 cggaccatca acgatctgta tttcgccgtg tcggtcaacc agacggtttt gccggccagt
     7801 gcggtggcaa ttgcgttgct cgcgggattc ctgaccgcgc tcgtgggggc gctggtccca
     7861 gcactggagg ccgcgggcgg tgctccgcaa ctgggactca ggcgctcggt gctcgagtcg
     7921 cgcgccgtcg ccatcgcggg ccgactggcc ctcgccagcc tcgcgctggc gattggctca
     7981 gccacgctcg cagcgagctc cggacgcagc ctgctggcag gattcgttgc gctgttcatg
     8041 cttttgctga gcgtcgcagc actcacgccg gcgctgctgc gtgctggagc ccaggcggcg
     8101 gccaaagtct gcgctcgtgt cagtccggtc gcgcggctcg cctttgggga catcgccgcg
     8161 tcactgagtc gaacgggtgt ggcagtcgcc gcgctcggga tggcgatagc tgcgatgatc
     8221 ggcgtctcca tcatggtcga aagcttccgc gagtcgctgc gcgaatggtt gcagcaaacc
     8281 atacgcgctg acatctatgt gacggccccc ggcgctgggg gaccggagcg gcgcatcgac
     8341 ccgcagttgg tcgcgcagct gctgggaaca ccgggaattg ccgactacag tgtcagccgg
     8401 cacgcgagtc tcgaagtgag aggtgagaat gttgcgctcg atgcgttgca gcctgccgct
     8461 tcaagtcgtc cggggctggt attcgtgcag ggcagtgcgc aaaccgcctg gtcggggctg
     8521 gcgacggggg cgattctggt gtccgagccc ctcgcctggc ggatgagcct gcaggcgggg
     8581 caggacctca cgcttccaac actctccggg ccgcgtaact tcaggattgc cggcgtctat
     8641 cgggaatacg gcaacgatcg cggcaacctg atgatgagtc tgagcgagta ccggcgcttg
     8701 tggcatgacg acagcatcac cgggctcggc atctaccttg ccgcccacac ctcgcccgag
     8761 caggcaatcg ctcgactgcg ggcgaaggct cagggcaagc aggcgttgct gatcagctcc
     8821 aatacccggc tgcgggcgct ctcgatggat atcttcgagc ggaccttcgt gatcactcgc
     8881 gtgctttact ggctggccgc cggcgttgcg gcggtcggat tgctgagctc gttgctggcc
     8941 tgggagctcg agcgctcgcg cgaggtgggg ctgctcaggg cgctgggaat gacaccgcgc
     9001 ggggccgctg tgctgatcga ggtgcaggcc ggattcatgg ggctgatggc gctgctggcc
     9061 gcgattccgg ccgggctgct cacggcgctc atgctgatcc gggtcatcaa ccggcgcgcc
     9121 ttcggctggc agatcgattt gcatctcacg gcggcgcagt ttggcaacgc gctgctgcta
     9181 gccgttgcgg cggcgctggc cgcgggactg tacccggcat ggcgcagcgc acgcgccccc
     9241 atcgcggatg agatccggga cgagtgatgc gcggtcaggg acgtgttgcc acgaagtggt
     9301 gctcgaggct tgcagcgctc atgctgggga tgctcgcagc tcagatcatg gcagctgcgg
     9361 gctcgctctc agagctcgat gtcctgcgcg gggtccgggc aaagagcggt ttcgagctcg
     9421 ccctcgcgcc gcgagaattc cactttccgc tcgatcacgg cccgcatccc actttccgcc
     9481 atgaatggtg gtacgtcacg ggaaatctcg atgccgccgg aggggcgcgt ttcggcttcg
     9541 agctgacatt cttccgcttc gcgctggcgc ccggaaacgg gactgacacc cctgcgcgct
     9601 cgcagcagtc acgctggcga gcacggcaga tctacctggc gcatttcgcc gtgacggacg
     9661 ttgagttggg ggtgtttgag tttgcgcaga aatattccag agaggcgctg gggctggcgg
     9721 gcgctcaggc cgagccgttt cgcgtctggc tggacgattg gactctggga ggagggggcg
     9781 gctcctggaa cttgagcgcc cggggccagg gctatgagct gtcgctcgat gcgcaactac
     9841 tcggcgcccc ggtgctcaac ggccaggccg ggttgagccg caagtccgac gagcctggcg
     9901 cggccagtta ctactactcc gttccgcgca tggcggtgac cggtcggctc gtgcgcggcg
     9961 gccatccgat gcaagttcac ggcctggcct ggctcgatcg ggaatggggc agcggggcgc
    10021 tgggcccgca ccagcagggc tgggactggt tcgcactcca gctgcataac ggcggcacgc
    10081 tgatgtttta tgcgttgcgc gatggcaatg gtgcgcggga ccctcacagc gctggaacct
    10141 gggtgagccc gaccggcgag gtgagagcga ttgcgagcga ggacgtccag atagaggtac
    10201 tcgaccactg ggtcagcccg cggggtgggc gctatccctc gcggtggcgt atgcgagtac
    10261 ccgtggtggg catcgatgtc gagattcgac ccgtgttagc caaccaggaa ctggttacgc
    10321 gcccgcgata ctgggaggga gctgtggatg tcagcagcgc gcagggcggc tcggccgctt
    10381 ccggtcgtgg ttatgtggag ttggtcggct acggcgagta aattgcacgt atggattaca
    10441 gcagactgat ccaacgtgtc cgcgccctgc tggtctcgcc gcggaccgaa tggccggtga
    10501 ttgcggccga gccggtcacg atcgccgatc tgtaccgcga cccgatcgcc gtgctcgctg
    10561 ccatcgggcc cgtctgcgcc ttcatcaaga ccagcctgat cggctacggc tggcatggct
    10621 tccgagtcta ccggctgggg attggcgccg gcctcgcggc cgcgatcgtc agttacgccg
    10681 tcacgctcgt ggcggtttat gtgctggcgg tggtcatcga tttgctcgcc cccagcttcg
    10741 gtggacagca gaatcggctg caggcgctga aagtggcggc gtggtcgtgc accggcgtct
    10801 ggctggcggg attcgcgcat ctgctgcctt ctttcagtgt gttgctgttc ctcgccggag
    10861 atgcttacag cgtctacctg ctgtacaccg ggctgcctgc gctgatgcag gtgccaccag
    10921 agcgctccgc ggcctacacg gccgcggctg cgatcacagc gctcgtgctc gggtggatcg
    10981 tctccctgct gacaggggga atcaccggtg tgcgtttcgg cctggattat tgatggcgga
    11041 gcgaatctct caggcgaact cagcaaggat cgccggcacg tgacggcgca gcaggaggtt
    11101 atgtgcctgc caacgctcgt tgggctcgag ctcctgcaaa aagcgcactt caccacctga
    11161 gaagaccgcg atgggcgctc catcacgata gagcacacgg ttgccactga gcgcgggcag
    11221 acgggcaccg ggtgtcacga tcccgaccag gttgagcggg tcagcggccg agagtgatac
    11281 gtagctctgg gtgtgcgctg cgcggcggac gtcgcgcagc agaccgatgg cttccgggac
    11341 ggcgaactgc tctcctgtga gcccggaaac gaaccggccg ccgcgaatct caccgcgtgc
    11401 ttccaaccga cgcaggcaca tgagcagatc gcgccagggc ggcagccagt cggcctcgcg
    11461 ggccaatagg cgccagaaca ccacgcccca gcgacgcagg agcactctgg tcacgtgttc
    11521 gatcgtttca tcgtcctggc gacgctggac caacgccggg gaggctgtct cgtccacctg
    11581 ctcgggtccc gcagccgggc gccgatgcac gagcgcccag cgtcccgccg catccatccc
    11641 gaagagcgag atcctgcggc gtcgccgccc gcccacagcg ggcctgcggc gatccgatgg
    11701 tacgagcagc gcgcgcagtc caccgaagct gtccgagttg acgacgccga gcgccacgag
    11761 ctctgcgagt gcctcctccg cctgcgccgg caacaacccg gctccatcgg cgatctcatc
    11821 gaagaacgaa gcgccgtggc tcctgatgta gtcggcgacg agacgtgccc tggcggacag
    11881 cggccgctcg tccgccggtc ccgaaagaga ggaccagatc cggatgttgc gtcgggacag
    11941 gagtgcgatc ggcgtggcgc ggaccggggc tgcgccgcgc tcggaatcat tcttccgctg
    12001 ccccgaggct cggtgcgcca gccgcgtcca cacgaagcgt cccgcgaggc actgctcatc
    12061 gagccaggcg ggctcgtact cgctgatgcg cgccggcagg atttcggtct cccaggcgct
    12121 ggcgggagcc tcgaacccct cgagctgccc gaggaccgcc gctaccgcgt cgggcccttc
    12181 catccgacct tccggcgtca ctctctgcca ctcgaacagg aatcgcagga aatcgcgcgg
    12241 ctgaatcggc tccacttccg cgcgcaaccg cttgacggtg taacggtgga tgcgggcgag
    12301 aaggcggcgc tcgcaccact cctcctccat cgccgagggc tcggcgctcg aagtgaatcg
    12361 cccgcgcatc gcgaaaccct ccgtctgcaa tgcagcgagc gcggcttcga ttgcaccgcg
    12421 ctccactccc acggagtgcg caagggacgt gacggttacc ggtcccaagc cttccagacg
    12481 gccgcgaacg gtctcgacga gcgcttcctc gcgggtcagg ctttgcgcgt aggcggccgg
    12541 cacctcgatc gccggatgcg gttcgacatc aggaaagatc gcgcgaaaga gcggcacacg
    12601 ctccgtggcc agccagagca cctgggcggg cggggcggcc tgctcgcgtt taggagcatc
    12661 cgacgggcag atctcgagcc gggtaatgcg tctttgtaac agcaggtttt gaatcaactc
    12721 gggccagccc gcgtgagagt cgacttcagc ctgggtgaga aaggttagcc aaaggagcgc
    12781 atcgtgcagc tcgtcctcgt tcgcagcgtc gggccacgct tcctcgcgca ctcgctgaat
    12841 tgcctcggga tcgagtctgc cgatatcgga tgcactctgc ggatcgagcc agcggcgact
    12901 catgactgcc tgagtgcgcc gctcctcgag cggtgcgtcg tccaaaaacg catacgggcg
    12961 agccgagagg acttccagcg ccagtggcga gggctccgtg acatcgcgcg ccaccacctg
    13021 aatctcgccg cgctcgagcg cgcgcagcag gtgctcgaag ccatcgatgt ccatggcttc
    13081 ctccaggcaa tcgcgaatcg tctgccggat gagcggatga tccggtatct cgagctcccc
    13141 cggcagattt tccgcgcacg cgagctggtc gggaaagatt gaggtcagca ggtcctctgc
    13201 cgccattcgc gccaggggcg ccggtacctt cttgccgccg cgaaatcgtg gcagcgcgag
    13261 tgcgacggtt gcgcaccagc gccagcgagc tgtgaacatc ggcgctgcac acagtgcctg
    13321 caccaggatg gctctggcgg tgttggaatg caggtagcgc gcgctgtcgg ccagctcgaa
    13381 gctgtgtgcc gtggtcagcg acaggatgat cgtgtcctcg gtggctgcgg cctgcagctc
    13441 gaaattgaac gagcggcaga accgcttgcg caacgcaagt ccccaggcgc gattcaccct
    13501 gctgccaaag ggagaatgaa tcacgagctg catgccgccg gcttcatcga agaagcgctc
    13561 gaacacgatc ctttgctgcg tgggcaacgt cccgagcgca ccgtatccgg ccgccagata
    13621 gcccgccagc tgcgccgccg caggtccgcc tacaccgacc tgatcgatca gccagcgctg
    13681 tgcggagtcg agtccctgcg gcagcaggct ctcgatctcc tgccgcagac gcgacaccgc
    13741 gaatgacagc tcgtcggagc ggcccggggc ttcaccgagc cagaacggga tggagggcgg
    13801 ctgaccgtgc gcgtcctcga cgcgtacctt tccctgctcc acgcgcagaa tgcgatagga
    13861 cacattgccc agctgaaaga tgtcgccctg caggctctcg acggcgaagt cctcgttgac
    13921 cgtgccgacg aaagtggctt gaggctcgag aatcacctgg taatcggccg catcgggaat
    13981 cgtgccgccg gaggtaatgg ccgtgagccg cgccgctcgc cggccgcgca gcctgtgatt
    14041 caccgggtcg tgataaatga gcgccccatg gcgcccgcgc cgcgtgctgt agcccgtggc
    14101 cagcatccgc acgatctcat cgaactcctc tcgcgtcagg tcccgatagg ggtaagcgcg
    14161 ccggatcaga ttgaagaggg cttcttcatc ccactcgagc gccgccacct cggccacgat
    14221 ctgctgtgcc agaacatcca gcggcttcgc cggcatcctc agccggtcca gctcggcgcg
    14281 ccgcaccgca tcgagcagcg cgccgcactc caccagatcg tcgcgtgaga gcgggaacag
    14341 ccgacccttc ggtgtgccgc cgaccgagtg gcccgatcgc ccgacccgct gcagcagcgc
    14401 cgcgatcgag cgcggggaac cgagctggca gacgagatca acatcaccga tgtcgatgcc
    14461 gagctcgagg gaagcggtcg ccacgagagc cttcagctcg cctcgcttga gacggctttc
    14521 ggcatccagc cgcagctcct tggccatgct gccgtgatgt gccgtgacct gagtctcgcc
    14581 aactcgctcg gagaggtggc gagcgactcg ctcggcatgg cgccgggtgt tgacgaagac
    14641 cagtgtcgtg cgatgctcgt tgacgagctc cgcgagccgg tcgtaaacct gggtccacag
    14701 gtcacccgac atcacggcct cgagcggcga gggcggcatc tccagcgcca gatcgcgggc
    14761 gcgaacgtgg cccgaatcga tgatgtcgca atcggcggtg ccgtcggagc gcagatgtcg
    14821 catgccaacg aggaatcgcg ccacatcttc gatcggtttc tgtgtggcag agagcccgat
    14881 gcgtgtcagc ggagcgccgg tcagcgctgc gagccgctcg agggaaaccg cgagatgcac
    14941 gccgcgcttg ttgcccacca tcgcatggat ttcatccacg atgaccgtgc ggatgctcgc
    15001 cagcatcttc ctgcccgact ccgatcccag caggatgtac agcgactccg gagtcgtgac
    15061 gacgatgtgc ggcgggtgcc tgcgcatact gttgcgctcg tgctgcgggg tatcaccggt
    15121 ccgaacgaac gtccggatgg cgatctccgg caggcccaac gccaccagct cttcgcggat
    15181 gcctgccaga ggcgcttcca ggttgcgccg gatgtcgttg gagagcgcct tcagaggaga
    15241 gacgtacacg atccgcgtct catcgctcag agcgttctgc aagccctcgc ggatcagccc
    15301 atcgagcgcc gccaggaacg cggcgagcgt tttgcccgat ccggtcgggg ctgcgatcag
    15361 gacattccgg ccggcctgaa tggccggcca cgcctgctgc tgagggggcg tcggcgcatc
    15421 gaaggtgcgg ctgaaccagc tggctacggc ggggtgaaaa gaggaaagga cgctcatcct
    15481 cgacctgccg cgcacggcac ggcgaacgac ttcatgggat gatcggaatt gaaccacaga
    15541 atcccgcggg ccggcagagg ccggcgcgag agttcatact gcgtacagta ccgctcgagc
    15601 gcgcctgctg tgcgggccct gaacagagtt tgattcactc gcgacagcag gcaggcaagt
    15661 cgatccaggc gccatcccag acggg
//
LOCUS       DASRHN010000010         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2146672, whole genome shotgun sequence.
ACCESSION   DASRHN010000010 DASRHN010000000
VERSION     DASRHN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146672"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFB37_00385"
     CDS             complement(<1..454)
                     /locus_tag="VFB37_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO20925.1"
                     /translation="MAHALIVDDDPDVVDWLQEVARMEGFTVARAHSLRDARIELGRQ
                     RPDVLLTDLRLPDGEGIELVRELEKPEATEVIIVTGHATVDSAVAALRAGASDYLVKP
                     ADLERVQAVLRHAKKTSALQHEIGALRDELRKLGRFGRILGSSPRMQVL"
     gene            complement(787..1980)
                     /locus_tag="VFB37_00390"
     CDS             complement(787..1980)
                     /locus_tag="VFB37_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZO20926.1"
                     /translation="MRRPDNTRADLPLTRWSPHPILLGLGVGLLAGPAGLHLLEPHVV
                     EDSGQVESISEIVLVVALFCAGLRLRVPFEWHFWRIPLRLASLTMLATATLAAAAAHV
                     LFGMNLVEALLLGVVLAPTDAVLASDIHVPAEIDQDVVPFTLAAEGALTSTLAAPLLL
                     VVLEILGFGDSGSDALGAHLALSIWSVVGGAVVGWLIGALMMRWLTLLDSDRQGDFLE
                     DMVVVATAVLTYVSALALRADGLIAVFVAGLALSHGGRLRRSLRKATPSARVLRFAGR
                     AERFAAVLVAVLLGALLAGADLHLRMEVFALLLLAGLRPLAVRLGLGGLGLPPAQRRP
                     LEWFGARGAASLYCLELAINRGLGGEFAHELAATALVVVVTSIIFSAVSAFSLRKASP
                     GPGIV"
     gene            1940..2500
                     /locus_tag="VFB37_00395"
     CDS             1940..2500
                     /locus_tag="VFB37_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20927.1"
                     /translation="MSGRSALVLSGLRTAARPGTGQWLPLAAQAPDLIVVGHHILELA
                     QHDRSHEARQGIVQSVPVDLERSAFATLTLIENNLIFVTSKSGLQSGLQLRPAAMGSS
                     RLTRTGFVRISAEQAYQRLQFLSGFAALLQMRPKQPSKSRPTSMLRAHSEPALGVAPS
                     RDQLLQRADGETMTHDDAFVVRPTRN"
     gene            complement(2975..>3178)
                     /locus_tag="VFB37_00400"
     CDS             complement(2975..>3178)
                     /locus_tag="VFB37_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20928.1"
                     /translation="DEDGYALVRKLRALEDGRHVLAIAVTGYASKGDVAAAMAAGFDL
                     HVPKPVDFDTFVPMVRRLAALRH"
BASE COUNT          625 a         1075 c          966 g          512 t
ORIGIN      
        1 aaaggacttg catgcgcggg gagctgccga gaatccggcc gaagcggccg agcttgcgca
       61 gctcgtcgcg caacgccccg atttcatgtt ggagtgccga ggtcttcttc gcgtgccgca
      121 acaccgcctg cacgcgctcc agatcggcgg gcttcaccag gtaatcgctc gccccggcgc
      181 gcaaagcggc aacagcacta tcgacagtcg catgaccggt gacgatgatc acctcagtcg
      241 cttcgggctt ttccagctca cgaacgagct cgatgccctc gccgtccgga aggcgaagat
      301 cggtcaacag gacgtccgga cgctgccgtc cgagctcgat gcgcgcatca cgcaggctgt
      361 gcgcccgcgc gaccgtaaag ccttccatgc gcgcaacttc ctgcaaccag tccacgacat
      421 ccggatcgtc gtcgactatc aaagcatgtg ccatgtcatc ccctgaacca attcttcgaa
      481 aacctcactc tagactcaag ctgccgcgag cgccgcattg tcgtccccac gcgatgggaa
      541 cgatcgatgc gagctcgacc tgtccacgaa agttaaccca tatcagtctg cctcgatggg
      601 ctggcgactt ccgcgtaacg gcgcggcagt gtgttttgcc tgggttggcg gctatggtgc
      661 tcgggtcgtc tgcgtcgacg gcactcccgc gagggtacca tggccggcaa tgggcgagat
      721 agccaaacgc ccgtcgagct gccaagttcc cgcaactcat tctcgggcgg agccggatcc
      781 tcgtacttat acgattccag gtcctgggga cgctttccgc agggaaaacg cggagaccgc
      841 actgaagatg atcgaggtca ccaccacgac cagggccgtc gcggccaact cgtgcgcgaa
      901 ctctcctccc agtccgcgat tgatggccag ctcgaggcag tagagcgagg ccgcgccacg
      961 ggcgccgaac cattccaatg gccggcgctg ggccggcggt aagccgagcc cgccgagacc
     1021 aagacgtacg gccaaggggc gcagcccagc gagcaacagg agggcgaata cctccatccg
     1081 caaatgcaga tccgcgcccg ccagcagcgc accgagcaac accgcgacca gcacggccgc
     1141 gaagcgctcc gcgcggccag cgaaccgaag cacccgggcg ctgggggtcg ccttgcgtaa
     1201 cgagcgacgc agacggccac cgtgcgagag agcaagcccg gcgacgaaca ccgcgatgag
     1261 gccatcagcc cggagggcca gcgcgctgac atacgtgagc accgccgtcg ccacgacgac
     1321 catgtcctcg aggaagtcgc cttggcggtc ggaatccagc agggtgagcc aacgcatcat
     1381 cagtgcgcct atcagccaac ccaccaccgc cccgccgaca acggaccaga tgctcagagc
     1441 caagtgggcg ccgagtgcat cggagcccga atccccaaat cccaggatct ccagtacgac
     1501 caacagcagc ggagcggcaa gcgtactggt cagcgcgcct tcagcggcca gcgtgaaggg
     1561 caccacgtcc tggtcaatct cggccggaac gtggatgtcg gaagcgagca ccgcgtcggt
     1621 cggtgcaagc acgacgccca acaacaacgc ttcaaccaaa ttcatcccaa acaacacgtg
     1681 cgcggcggct gccgccaacg ttgcggtggc cagcatcgtc agagaggcca gtcgcaatgg
     1741 gatccgccag aagtgccatt cgaacggcac ccgtagccgc aggccggcgc agaacagcgc
     1801 cacgaccagc acaatctcgc tgatggattc gacctgcccg ctgtcttcca ctacatgcgg
     1861 ctcgagcagg tgcagccccg cgggcccggc cagaagtccc actcccaggc cgaggagtat
     1921 ggggtgtggc gaccagcgcg tgagcggcag gtcggctcta gtgttgtccg gcctgcgcac
     1981 cgctgcccgc cccggaaccg gtcagtggct gccattggct gcgcaggccc cggatctgat
     2041 cgtggttggt catcacatcc tcgaactggc gcagcacgat cgctcgcacg aggcccggca
     2101 agggatcgtc caaagcgttc cggtagacct cgagcgctcg gctttcgcca cgctcacact
     2161 catcgagaat aacctcatct tcgttacaag caagagcggt ctgcagagcg gcttgcagct
     2221 ccgtccagcg gcgatgggca gcagccgact cacgcgaacc ggtttcgtcc ggatctccgc
     2281 cgagcaggcg taccagagat tgcagttcct gtcgggcttc gctgcactcc tgcagatgag
     2341 accgaagcag ccctcgaagt cgcggcccac gagcatgctc cgcgcacatt cggagcccgc
     2401 gctcggcgtc gcgccgagcc gcgatcagct ccttcagcgc gccgatggtg aaacgatgac
     2461 tcatgatgat gcctttgttg ttcgaccgac gcgcaactga gcgcgcgtga gcctcagcca
     2521 cagcacgaga tgtgccgttg cagcgcggcg gacgtggtga gcgtcactcc ggctgcgcac
     2581 gcacattttt caccaccggt acgaattaca ccggcttttc gcgcctggca gggctcgatc
     2641 gcctggaatc tcccggcttt agtagggcaa gccctatggg agaacgacct ccggaggccg
     2701 caattcgttc aaaccgacct caaaaccgcg ggactcccgt tcccaggcgc cttcaggccg
     2761 tattttttga cactccagag cgcatggcat gcccgttgca tcgggggaac tacgggtatg
     2821 ggagttgcga gcttcgcaac tcgaaacagt tttgcgctga cgccgcaatc agggcggcag
     2881 tgcttaccga agaccccccg tttggcgggc tgtggtgcga ccagcccgct atttttttgt
     2941 gctgaagatt tggttggggg atggcttcac cgctctagtg tcgcagcgcg gcgagcctgc
     3001 ggaccatagg cacgaaggtg tcgaaatcaa ccggcttcgg tacgtgcaga tcgaagccgg
     3061 ccgccattgc cgcggccaca tcgcctttcg aggcatagcc cgtcaccgcg atcgcgagca
     3121 cgtgccgccc gtcttcgagc gcccgaagtt tccggaccag cgcgtaacca tcctcgtc
//
LOCUS       DASRHN010000011         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2146678, whole genome shotgun sequence.
ACCESSION   DASRHN010000011 DASRHN010000000
VERSION     DASRHN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146678"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFB37_00405"
     CDS             complement(<1..446)
                     /locus_tag="VFB37_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20929.1"
                     /translation="MSSDDDAGDLPAALEDIVSTGAAADAGDDEDRLKGLPRLLRYAM
                     LSTNRAQHVVERLVAEIHELCPNLPQSAAWALERTFDTGVVLAAELDHRAVTQDEPNL
                     RSLADCVRLLSLPTPRNSRQLEEHQRVGKALVHAFFESLGHVEKGL"
     gene            complement(443..667)
                     /locus_tag="VFB37_00410"
     CDS             complement(443..667)
                     /locus_tag="VFB37_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20930.1"
                     /translation="MYDATEAQRLRIQQLLLDERAMPLGQLLKAVAGESGPAAAVMAL
                     ACANLLSLDLLTRPLDPITLVRCCDPAERA"
     gene            complement(892..1692)
                     /locus_tag="VFB37_00415"
     CDS             complement(892..1692)
                     /locus_tag="VFB37_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20931.1"
                     /translation="MEADVGDGWRDLLERLCGRMETALQPGETIRITQVVKKSASLRV
                     YWRGDTSADTVARLHEAVALAKARSACTCESCGKEGRLCILGEFSMIRCANHLEGELI
                     PAPLGQENVHLVRVRAADGYRIVARRYDREADRFLDETPTIPDDRRPETAVTASWQAD
                     VMQAHPRLFRIPRDEPTSSPGYPRCSQGWRDLLDQLCSRIEDALGENETFEFTRIDDK
                     LSLLLIRWIGEVSRETRVKIEEAIHLASRRAGRTCSTCGLDRSAATQA"
     gene            complement(1776..>2435)
                     /locus_tag="VFB37_00420"
     CDS             complement(1776..>2435)
                     /locus_tag="VFB37_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20932.1"
                     /translation="RSGARCRGSAMSNMITPGQEPRQNGAASPSDHAGEGGAPSAVSI
                     ACRETAKPPRDGGDAKILDISSPVVRALLQIAPLRWWRSMPADAFGATEYLAIRSALD
                     EVAMLIEGAEVEPALEGDADAAIALVSSLTPIRRPGGIKSDIAMTAVLRLALEGEPRC
                     ALVLAHIIDRAEPDPRQADLLCGSWFEFRCGHAALCGGFTQGERAVLNALRAVEAAGG
                     L"
BASE COUNT          403 a          830 c          784 g          418 t
ORIGIN      
        1 tcgagcccct tctccacatg cccaaggctc tcaaaaaacg cgtgaaccag cgctttgcca
       61 accctttggt gctcctcgag ctgacgagaa ttgcgcggcg tcggcagcga cagcagccgg
      121 acgcagtcgg caaggctccg caagttcggc tcgtcttgcg tgacggcacg atgatcaagc
      181 tcggctgcga gcaccactcc ggtatcaaac gtgcgctcca aggcccaggc ggcgctctgc
      241 ggcagattcg ggcacagctc atggatctcg gcgaccagcc gctccacgac gtgctgggct
      301 ctgttggtgc tcagcatggc atagcgcagc aggcgcggca gtcccttcag gcgatcctcg
      361 tcgtcacccg catcggccgc cgctcccgtc gagacgatat cttcgagggc tgctggcagg
      421 tcacctgcgt catcatcgga gctcatgcgc gctccgccgg atcgcagcat cgaacgagcg
      481 tgatcgggtc caacgggcgg gtgagcagat cgagcgagag gaggttggcg caggcgagcg
      541 ccatcaccgc cgccgcggga ccgctttcgc cggccacggc tttgagcagc tggccgagcg
      601 gcatcgctcg ctcgtcgagc aggagctgct ggatgcgcag cctttgggcc tctgtcgcgt
      661 cgtacataat actactctct cctcctacag ggatggacga aagcgcgctg ccgccgtcgg
      721 gtcatcgaga tcagccatgg cttgctccag gcctcagatc tcgacaatgc tgtgcggagc
      781 gggcccggca ctcaaatcgg gtcattgctg tcgctccggt cgcgcgcagg cgacgcactc
      841 tcgcccagcg agccgtgcat gtcgtcgttg aatgcaggcg tggaacagtc ctcatgcctg
      901 cgtggcggcc gatctatcca ggccgcaggt tgagcaggtc cggcccgcgc gtctggaggc
      961 gaggtggatg gcctcttcga ttttcactct ggtctcgcgc gagacttcgc cgatccacct
     1021 gatcaggagc aggctcaatt tgtcgtcgat ccgagtgaac tcgaaggttt cgttctcccc
     1081 gagcgcgtct tcgattctgc tgcagagctg gtccaggaga tcgcgccacc cctgactgca
     1141 gcgcggatag cctggggacg atgtcggctc gtcccgcggg atccggaaca gccgaggatg
     1201 tgcctgcatg acatcggcct gccaactcgc ggtcacggcc gtttctggcc tgcggtcgtc
     1261 cgggatcgtc ggggtctcgt cgaggaagcg gtcggcctcg cggtcgtaac gccgcgccac
     1321 gatgcggtag ccgtcggccg cccggacgcg aacgagatgg acgttctcct ggcccaaagg
     1381 cgccgggatc agctcgcctt ccaaatggtt ggcgcaacgg atcatggaga attcaccgag
     1441 gatgcagagc cggccctcct tgccgcagga ctcgcaggtg caggcggagc gtgccttggc
     1501 aagcgcgacg gcctcgtgaa gtctggcgac cgtgtctgcc gatgtgtcac cgcgccaata
     1561 gacccgtagc gatgccgatt tcttgacgac ctgcgtgatc cggatcgtct cgcccggctg
     1621 gagggccgtt tccatgcgcc cgcagaggcg ctcgagcaga tcgcgccagc catcgccgac
     1681 gtcagcctcc actcttggcg ggtcgctcac cgggtcgaat agatccggat gcgccaacac
     1741 gagctcacgc cgccaatccc gcttctcata cgccattaca gacctcctgc cgcctcaact
     1801 gcgcgcagtg cgttcagcac cgccctctcg ccttgggtaa agccgccgca gagagcggca
     1861 tgaccgcagc ggaattcgaa ccaggagccg cacagcagat cggcctgccg cggatcgggc
     1921 tcggcgcgat cgatgatgtg ggcgagcacg agcgcgcatc tgggctcgcc ctcgagcgcg
     1981 agccggagca cggccgtcat tgcgatatca gacttgatgc cgcccggccg cctgatgggc
     2041 gtcagcgacg acaccagtgc gatcgcggcg tccgcatcgc cctccagcgc cggctcgacc
     2101 tcggcgccct cgatcaacat tgcgacctcg tcgagcgcgg agcggatggc gaggtactcc
     2161 gtcgccccga acgcgtccgc aggcatcgac cgccaccaac gcagcggcgc gatctgaagc
     2221 agcgcgcgga caaccggcga cgagatgtca aggatcttgg catcgccgcc atcgcgcgga
     2281 ggcttcgcgg tctcacggca ggcaatgctg accgcgctgg gcgcgccgcc ctcgcccgcg
     2341 tgatcggaag gactggccgc accattttgc cgaggctcct gccccggtgt gatcatgttc
     2401 gacattgccg agcccctaca ccgcgcgccc gatcg
//
LOCUS       DASRHN010000012         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2146756, whole genome shotgun sequence.
ACCESSION   DASRHN010000012 DASRHN010000000
VERSION     DASRHN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146756"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VFB37_00425"
     CDS             complement(<1..1325)
                     /locus_tag="VFB37_00425"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20933.1"
                     /translation="MNRNRVAAVAGMLCALLMMYAGAVSAQVDSQLFSGMRWRQIGPF
                     RGGRVVAVSGVPGDPATWYFGAVAGGVWKSSNAGDTWQAVFDGQKIASIGAIAVADSN
                     HDILYVGTGEACPRGNITYGDGVYKSVDAGKTWEHMGLDDTRQIGAVIVDPRNPEVVL
                     VAALGHAFGPNEQRGVFRTADGGKTWQRVLYRDRDTGAIDLAFDPHHPDVVFASLWQM
                     RRQPWSFSSGGPGSGLYRSSDNGLTWKQIQGHGLPAAPLGRIGISVSGADGQRVYASV
                     EAKEGGIFRSDDGGANWSRINEDHRFRQRAWYYSHIFADPVARDTVYVLNTGAFRSTN
                     GGKDFQLLPAPHGDHHALWIDPRDPRRLINGNDGGATISVDGGATWTQQNNQPTAQFY
                     HVAVDNHWPYRVYGAQQDNSTVAIASRDDAGVIGRQDWYEVGGGESGYIA"
     gene            1482..2225
                     /locus_tag="VFB37_00430"
     CDS             1482..2225
                     /locus_tag="VFB37_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20934.1"
                     /translation="MRALAILVMVVASPCVLAFTAEELASRNIAAHGGAEKLNAIRTL
                     RLTGKLLLNGGTLEVGYTMLIKRPDSIRYEAALQGLTRVQAYDGKQAWQINPFQGRKD
                     PEKLSGDDAKGLGEDAADFTGPLVDYKAKGYKLDYLGTEDVDGTEAHKLRVTRPNGDI
                     VYVYLDPDYFLEIRTVERRIEHGVPSETVTDYGDYEKVDGAYFAFAQESGPKGSSDRQ
                     KVQIDKAEANVPADDAEFRFPAPRTASAK"
     gene            2246..>3879
                     /locus_tag="VFB37_00435"
     CDS             2246..>3879
                     /locus_tag="VFB37_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20935.1"
                     /translation="MRSGLAMVSCVLACSWSIAAVAADPPVIDSGTVSGLGARNIGAA
                     AMSGRISALAGSDDPSGKVTLFVGAASGGVWKSTDGATTFKPVFDDQPVQSIGAITID
                     SSHPQNVWVGTGESWTRNSVSVGDGIYRSTDAGETWTHVGLENSERISRILVDPRASE
                     TVYACVPGKLWSDSADRGLYKSSDGGKSWQLVLKGSNLSTGCGSVAMAPGKPDVLLAS
                     LWDFRRKGWTFRSGGDGPKAASGSGLYRSDDAGKTWVELTAQPDHGLPAKPFGRIALA
                     FAPSDANTVYAFIESTDSGLFISHDGGRSWERGDKSQWMVWRPFYFANLIVDPKDARR
                     VYKTDGSLILSEDGGKSFSTVGGFVGMHGDVHDVFIDPANPQHVFAGDDGGLWISFDG
                     ANKWWKADNLPISQFYHVSLDDADPYHVYGGLQDNSSWVGDSAYPSGIPSSRWENMFG
                     GDGFWMFADPSDHDYVYAEAQGGTIGRVNIHTHETRDIQPKLGPEDLKRYTKLRFNWN
                     TPIALSPHDPAAVYIGAQFLFRSPDHGQTWERISPDL"
BASE COUNT          711 a         1267 c         1239 g          662 t
ORIGIN      
        1 gcgatgtagc cgctttcgcc gccgccgacc tcgtaccagt cctggcggcc gatgacgccg
       61 gcgtcatcgc ggctggcaat ggccaccgtc gagttatcct gctgcgcgcc gtagacccgg
      121 taaggccagt ggttgtccac cgcgacgtga tagaactggg cggtcggctg attgttctgc
      181 tgagtccagg tcgccccgcc atccaccgag atcgtcgccc cgccgtcgtt gccgttgatc
      241 aggcggcgcg gatcgcgcgg atcgatccag agcgcatgat gatccccgtg tggggcgggc
      301 agcagctgga agtccttgcc accgttggtg gagcggaagg caccggtgtt gagcacgtag
      361 acggtatcgc gtgcgaccgg gtcggcgaag atgtgcgagt agtaccaggc ccgctggcga
      421 aagcggtggt cctcgttgat gcggctccag ttggcaccgc cgtcatccga gcggaagata
      481 ccgccctcct tcgcttcgac cgatgcatag acgcgctggc cgtctgcccc cgagacggag
      541 atgccgatgc ggcccagcgg ggcagccgga agcccatggc cctggatctg cttccaggtc
      601 aggccgttat ccgaggagcg atacaggccg gagcccgggc caccgctgga gaagctccac
      661 ggctggcgcc gcatctgcca cagcgaagcg aacacgacgt ccggatgatg cgggtcgaaa
      721 gcaagatcga tcgcccccgt atcacggtca cggtacagga ctcgctgcca agtcttgccg
      781 ccatcagctg ttcgaaagac tccgcgctgc tcattggggc caaacgcatg gccgagcgcg
      841 gcgaccagca cgacctccgg gttgcgggga tccacgatga cggcgccgat ctgccgggtg
      901 tcgtccagac ccatatgctc ccaggtcttg cccgcatcaa ccgacttgta gacgccatcg
      961 ccgtaggtga tgttgcctct cgggcaagcc tcaccggtcc ccacatagag aatgtcgtgg
     1021 tttgaatcgg cgacggcgat cgcaccgatc gatgcgatct tctgtccatc gaagaccgcc
     1081 tgccaggtgt cgccggcgtt gctgctcttc cacacgccac ctgccactgc gccgaaatac
     1141 caggtcgccg gatccccggg caccccgctc acggcaacca cccgtccgcc gcggaacggc
     1201 ccgatctgac gccagcgcat gccgctgaag agctgcgagt cgacctgtgc cgagacagcg
     1261 cccgcataca tcatcaacaa cgcacacagc atgcccgcga cagcggctac ccgattccga
     1321 ttcatgtgat tccccttcgc cggaagtcaa tcgaccgatc gcgcggcgca cacggctcgt
     1381 cgcgtttgca cgcaccgccg acacggcgca aggctccccg gactggtgcc cccgcgcgtg
     1441 gcagcgcatc atagccgcct tcagggagat acgagagtcc aatgcgtgca cttgcgatcc
     1501 tggtgatggt cgtggcatcc ccatgcgtgc ttgccttcac ggccgaggag ctcgccagcc
     1561 gcaatatcgc agcgcacggc ggcgccgaaa agctcaatgc catccggaca ctgcgcctga
     1621 cgggcaagct gctgctcaat ggcggcacgc tcgaggtcgg atacacgatg ctgatcaagc
     1681 gccccgactc gatccgctat gaggctgccc tgcaggggct gacccgggtg caggcctacg
     1741 acggcaagca ggcttggcag atcaacccgt ttcaaggccg caaggacccg gagaagctct
     1801 cgggggatga tgcgaagggc ctcggggagg acgccgcgga tttcaccggc ccgctggtgg
     1861 actacaaggc caagggctac aagctcgatt acctgggcac ggaagatgtg gacggcaccg
     1921 aggcgcacaa actgcgcgtg acacgtccga acggcgacat cgtttacgtt tatctggacc
     1981 cggactattt ccttgagatc cgtaccgtcg agcgccgcat cgagcatggg gtgccgagtg
     2041 agactgtcac cgattacggc gactacgaga aggtcgacgg cgcctatttc gccttcgcgc
     2101 aggagtcagg gcccaagggc tcgagcgatc gccagaaggt ccagatcgac aaggccgaag
     2161 ctaacgtgcc ggccgacgac gccgagttcc gctttccggc gcctcgtacg gcctcggcaa
     2221 aatgatccga caggagatga ggccaatgcg atcaggactc gcgatggtga gctgtgttct
     2281 cgcgtgctcg tggagcatcg ccgccgtcgc ggccgatccg cccgtgatcg actcgggaac
     2341 ggtttccggg ttgggcgcgc gcaacatcgg cgcggcggcc atgagcgggc gcatctctgc
     2401 cctggccggc tcggacgatc cctccggcaa ggtgacgctg ttcgtgggcg cagcgagcgg
     2461 cggtgtctgg aagtccaccg acggagcaac cacattcaag ccggtgttcg acgatcagcc
     2521 cgtgcagtcg atcggcgcga tcacgatcga ttcctcgcac ccgcagaacg tgtgggtggg
     2581 cacgggcgaa tcctggacgc gcaacagcgt ctcggtgggt gatggcatct accgctcgac
     2641 ggatgcgggg gagacctgga cacatgttgg tctggagaac tccgagcgaa tctcccgcat
     2701 cctcgtggac ccgcgtgcct ccgagacggt gtacgcctgt gtgcctggca agctgtggag
     2761 cgactcggcg gaccgcgggc tgtacaagag cagcgacggc ggcaagagct ggcagctcgt
     2821 gctgaagggc tcgaatctct ccaccggctg cggcagcgtg gccatggctc caggcaagcc
     2881 ggatgtgctg cttgccagtc tttgggattt ccggcgtaaa ggctggacat tccgttcggg
     2941 tggcgacggc ccgaaggcag cctccggcag cggcctgtat cgcagtgacg atgcgggcaa
     3001 gacctgggtg gagctcaccg cgcaacccga tcatggtctg ccggcgaagc ctttcgggcg
     3061 catcgcgctc gccttcgccc cgagtgatgc caacaccgtc tacgcattca tcgaatcgac
     3121 cgactccggc ctgttcatct cccacgatgg cggcaggagc tgggagcgcg gcgacaagag
     3181 ccaatggatg gtctggcggc cgttctattt cgccaatctc atcgtggatc cgaaggatgc
     3241 ccgccgggtc tacaagaccg acggcagcct gatcctgagc gaagacggcg gcaagagctt
     3301 cagcacagtc ggcggcttcg tcggcatgca cggcgatgtg cacgatgtct tcatcgaccc
     3361 ggcgaacccg caacacgtgt tcgccggcga tgacggcggg ctgtggatct ccttcgacgg
     3421 tgccaacaaa tggtggaaag ccgacaacct ccccatctcg cagttctatc atgtcagtct
     3481 tgacgatgcc gatccctacc atgtctatgg cggactgcag gacaacagct cctgggtggg
     3541 agattccgcc tatccgagcg gcattcccag ctcgcgctgg gaaaacatgt tcggcggcga
     3601 tggtttctgg atgttcgccg acccttccga ccatgactac gtgtacgccg aagctcaggg
     3661 cggcacgatc ggtcgagtca acatccacac gcatgagacg cgtgacatcc agccgaagct
     3721 gggccccgag gatctcaagc gttacacgaa actgcggttc aactggaaca cgccgatcgc
     3781 cctctcgccc cacgacccgg ccgcggtcta catcggcgcg caattcctgt tccgctcccc
     3841 cgatcatggg cagacctggg agcgcatctc gcccgatct
//
LOCUS       DASRHN010000013        10269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2146784, whole genome shotgun sequence.
ACCESSION   DASRHN010000013 DASRHN010000000
VERSION     DASRHN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146784"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1129
                     /locus_tag="VFB37_00440"
     CDS             248..1129
                     /locus_tag="VFB37_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZO20936.1"
                     /translation="MRARIVARLIHFFGPRSLRPILAAMKLRGLSIYQTPVAIGSSAP
                     AATLAGQAHAMPTSLEQVGARHRTSLGGNLRASVFGVNDGLVSNASLVLGVAGAGAAG
                     GYVLVTGAAGLLAGALSMAAGEYVSVRTQREMYEYQIALEREEVAEYPEEEAEELALI
                     YEARGVELEQAREMSRALLARPEQALDVLAREELGLNPDDLGSPIQAATASFLSFAAG
                     AALPLIPFLAHVPGQTALMATALLTALALFLIGMTLSLFTGRQALRGALRMVLIGGGA
                     GVVSFLVGRALGVALSP"
     gene            1215..2102
                     /gene="htpX"
                     /locus_tag="VFB37_00445"
     CDS             1215..2102
                     /gene="htpX"
                     /locus_tag="VFB37_00445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HZO20937.1"
                     /translation="MKRIILFLVTNLAILLVLSVVARLIGLDQYLAMRGDSLEGLLVF
                     AAFFGFVGSLISLALSKWMAKTTMGVQVITQPRNAAESWLINAVAAHARNAGVGMPEV
                     GVFDSPEPNAFATGARRDHALVAVSSGLLQRMNETEVNAVLGHEMTHVANGDMVTLAL
                     IQGVVNTFVIFLSRIIGNVVDRAVFRSDSGRGPAYFICVIVAQIVLGILASLIVMAFS
                     RWREFRADRGGAKLAGRDNMIAALDALKSAHEPLPQQFAAFGIADGSVSRGFQKLWMS
                     HPPLDERIAALRALPPNFA"
     gene            2139..3551
                     /locus_tag="VFB37_00450"
     CDS             2139..3551
                     /locus_tag="VFB37_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO20938.1"
                     /translation="MSATELVERLGADTVLTEAAAIEPFLADHRGLYRGRALAVVLPR
                     SVGQLSTLLALCNDRRTAVVAHGGNTSYCGGATPDESGRQIVVSLRHLNRIRNVDPLN
                     YSLVAEAGCVLSQVQRAADEAERFFPLSLGSEGTCQIGGNLSTNAGGTSVLRYGMMRD
                     LVLGLEVVLADGRLLSTLTTLRKDNTGYDIKSLFLGAEGTLGIITAASLKLFPKVRAT
                     ATALAAVTSVQAAVELLARLRESSGDRVSSFELIPRIAIDLTTRHIPAVLDPLGRPHE
                     WYVLCELSSSRAADALESLLEEFLAAAMEDRLVLDAVMTRSERERAALWKLRETIPEA
                     QRRDGASLKHDISLPVASLAAFVARAAQWIGTHVPDGRLVAYGHVGDGNLHFNLNQAP
                     GASREAFLGREAQVRRVLHDLVREFGGSFSAEHGIGRLKVEELERYAPPVELDLMHSV
                     KRALDPNGILNPGKVLRAQL"
     gene            3600..4529
                     /locus_tag="VFB37_00455"
     CDS             3600..4529
                     /locus_tag="VFB37_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO20939.1"
                     /translation="MVPGRLIIVAALLILSACSKSNGPAAEAPALTKDASSAGASVEG
                     APQIATEADGSHIEYRLYGHGEPAIVLIHGWATDANYWNAQLPVLKPRYSVVALDLAG
                     HGASSRNRTDWSMGNYGEDVASVVRRLPAQHVILVGHSMGGTVALEATRRIGDRVIGI
                     IVVDALKSVGLPPLGQREIEIRLAPFRSDFVGQTRKYVTESLFEKGADPVLVQKVAYD
                     MSLEPPEVAVPSMQALLGMNLASVLPDIHVPVMAINSDLAPTDEARIRKSLPDFKADV
                     LPHTGHFLMMEVPQQFNPILLRDIETLVAKAPH"
     gene            complement(4474..5583)
                     /locus_tag="VFB37_00460"
     CDS             complement(4474..5583)
                     /locus_tag="VFB37_00460"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HZO20940.1"
                     /translation="MLLMLAVAALATAGPSGKSDVDALAQIWPGIRDSSEQTFVGTDA
                     SVSPWGLGAERRVRTIVAPVSVPWLGAHVLYLEEFLHDEPDTVRRQLLLQLEPVEPPD
                     VGVEVHLFAFRDPHSWVHLDRLPTRVASLRRTDIAPVAGCDLRLKREADQFMGGTLGR
                     HCTDPREGSNRYVDYQLLIGPDLYWYRRRILSKPSEDVLEEVVGFNWFELNDARLFAC
                     RVEWSATGRRSDLRPLVRLDLHDQGGIGHFVTPDGRKLELTLHSQDWPFMPDRDALIL
                     LVQDQSETTPLATVWTQVDDDEISADLGWLKVSCGSLDPDTDEVWSSLQGLPEDPGRA
                     ARSPVQVLLQPGAAAVGLSAGLLPRASRYRAAGWG"
     gene            complement(5599..6477)
                     /locus_tag="VFB37_00465"
     CDS             complement(5599..6477)
                     /locus_tag="VFB37_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HZO20941.1"
                     /translation="MDPRLADLLKLLELEQLEINLFRGESRDIGSPQVFGGQVLGQAL
                     MAASRTIEGRLAHSLHAYFLRRGDPQAPIVYQVDRSLDGHSFSNRRVVAIQHGEQIFN
                     MAASFQVTEPGLDHQIAMPTVPSPESLADSSLLPAELLARMPERLRRFFEQPRPFEFR
                     PVQPIDFLRPRKAPPARQIWFRAVGALPDGEQLHRCLIAYVSDFFLLDTATLPHGASF
                     LTSPIVMASLDHAMWFHRPLRVDDWLLYAMESPSASGARGFARASVFARDGRLVTSTA
                     QEGLVRLKHAPGPGKT"
     gene            complement(6498..6968)
                     /gene="azu"
                     /locus_tag="VFB37_00470"
     CDS             complement(6498..6968)
                     /gene="azu"
                     /locus_tag="VFB37_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HZO20942.1"
                     /translation="MRLRRELIAAAMLVGLAMASPRAAHAAGDVCKLEITGNDLMQYD
                     RKLLALSGACTQVQLTLRHVGKLPKEAMGHNWVLVKAADLNAVANAGMAAGLQNNYIA
                     PGDKRVIAATRVVGGGESTSVTFSTAGLRKGESYMYLCTYPGHNSSMRGTFNFG"
     gene            complement(7010..8053)
                     /locus_tag="VFB37_00475"
     CDS             complement(7010..8053)
                     /locus_tag="VFB37_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO20943.1"
                     /translation="MSQTSRSPTSAEVVIIGAGPCGLFQVFELGLLGIPSQVIDSLAR
                     PGGQCAELYPDKPIYDIPALPVCGAQELVDRLLKQIEPFHPQFHLGQEVTEFTRRDDG
                     RFRVVTSAGTEFDAGAVVIAAGVGSFQPRRISLESADSFEGKTIHYRIRASSALNGQH
                     LVIFGGGDSALDWTLELAAKAASVTLVHRRPEFRAAPASVAKMRELVAGGRMRCFEAL
                     PHSLIIEEGELRGVNIKAPDGVVHALPAQQLLVFFGLHPKLGPIAQWGLELEKKALRV
                     DTEKFQTSLPGVFAVGDINTYPGKKKLILSGFHEAALAAFAIQHHLYPQKKQFLQYTT
                     TSPIMHQRLGVPD"
     gene            8252..9553
                     /gene="gabT"
                     /locus_tag="VFB37_00480"
     CDS             8252..9553
                     /gene="gabT"
                     /locus_tag="VFB37_00480"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZO20944.1"
                     /translation="MNSNSELHQRRQNAIPRGVSNSLAVYAERASNAELWDVEGRRYI
                     DFASGIAVLNTGHVHPKVKAAVAAQLEKFTHTCFQVTPYESYVALAEQLNEIAPGPTA
                     KKTIFLTTGAEAIENAIKIARFHTKRSAVIAFSGGFHGRTLACISLTGKVQPYKAGFG
                     PMLPEIFHVPYPMAYHGVSVEDSLEALSQLFKADVDPARVAAIIIEPVLGEGGFYAAP
                     AELLRKLRALCDQHGILLIVDEIQTGFGRTGRMFAIEYAGIEPDLMTIAKSIAGGVPL
                     SAVIGKAEIMDSPGPGGLGGTYAGSPLACAAGLAVLEVIREEQLLNRALHIGHFMSSR
                     LKGLQVRFPCLGDIRALGAMVAVELVRNNADSPDAELTRTLVQAAARKGLILLSCGIY
                     GNVIRFLAPLTIPDALLKEGFHLFEQALQEVASTTVAAPAA"
     gene            complement(9570..9869)
                     /locus_tag="VFB37_00485"
     CDS             complement(9570..9869)
                     /locus_tag="VFB37_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20945.1"
                     /translation="MTEIADTFDSAFTKAVDLGNRLADKDKDADLWDIADGLLAGALQ
                     YWLYSRQPCGDPRCQDCMPISTAEGRMAELKRLMEQLAAESEYFHTPTDANAGRA"
BASE COUNT         1744 a         3438 c         3312 g         1775 t
ORIGIN      
        1 aggacgacga cgaggacgac gagctcgatg aggacgagga cgagacggaa gacagggagc
       61 agtactgagc gtcccggaca gctggtatca cgagaaagag tcggcgtggc tctatcggca
      121 agttgcggcg gctgagcccc acgcgcatcg tagccggctg ttcctgcagc ttgccgctgc
      181 cgcggagcag caggccgaga gatggcatcc tgcgcactcc gccatccagc gacagttcgt
      241 gccctcggtg cgtgcgcgaa ttgtcgcccg cctgatccat ttcttcggac cgcgctccct
      301 caggcccatc ctggctgcga tgaagctgcg cgggctgtcc atctaccaga cacccgtggc
      361 gatcggatcg agcgcgcccg ccgcgaccct ggccggccag gcgcatgcga tgccgacctc
      421 gctggagcag gtcggtgcac gtcaccgtac cagcctggga ggaaacctcc gggcctcggt
      481 cttcggagtc aacgatgggc tggtctccaa tgccagcctc gtgctgggcg ttgccggggc
      541 gggagcggca ggcggctatg tgctcgtgac cggggctgcc gggctgctcg cgggagcgtt
      601 gtcgatggcg gccggcgagt atgtctccgt gcgcacccag cgcgagatgt acgaatacca
      661 gattgcgctc gagcgtgagg aagtcgccga gtatcccgag gaagaagccg aagagctggc
      721 cctgatctat gaggcgcgcg gagtcgagct ggagcaggcg cgcgagatga gccgcgcctt
      781 gcttgcgcgg ccggagcagg ctctcgatgt tctcgcccgc gaggagctcg gcctgaaccc
      841 ggacgatctc ggctcgccca tccaggccgc cacggcgtcg ttcctcagct ttgcagccgg
      901 agcggccctc cctctgatcc cgttcctcgc ccacgtgccg ggccagaccg ccctgatggc
      961 gaccgccttg ctgaccgccc tggcactctt tctgattggc atgacgctga gcctcttcac
     1021 cgggcgccag gccctgcgag gggcgctgcg catggtgctc attggagggg gcgcaggcgt
     1081 ggtgagcttt ctcgtgggcc gggcgctcgg cgtcgcccta agcccttgaa accacgtggc
     1141 tgagccccac ctacgccctg agccgcggta aaaagggcgc cccgacgggg gcttgcgcgt
     1201 tgcggaaggt caccatgaaa cgcatcatcc tgtttctcgt caccaacctg gccatcctgc
     1261 tggtgctgtc ggtcgtcgcc cgcctgatcg ggctcgacca gtacctggcg atgcgcggcg
     1321 acagcctcga agggctgctg gtcttcgccg ccttcttcgg ctttgtcggc tcgctgatct
     1381 cgctcgcact ctccaagtgg atggccaaga ccaccatggg cgtgcaggtc atcacgcagc
     1441 cgcgcaacgc agccgagagc tggctcatca acgcggtcgc cgcccatgcc cgcaatgccg
     1501 gagtcggcat gccggaggtg ggcgtcttcg attcgcccga gccgaatgca tttgccacgg
     1561 gcgcacggcg cgaccacgcc ctcgtcgcgg tcagcagcgg cctgctgcag cggatgaacg
     1621 agaccgaggt gaatgccgtg ctcggccatg agatgaccca cgtggccaac ggcgacatgg
     1681 tgacgctcgc cctcatccag ggcgtcgtca atacgttcgt cattttcctc tcgcgtatca
     1741 tcggcaacgt cgtcgatcgg gccgtgttcc gctccgacag cggccgcggg ccggcttact
     1801 ttatctgcgt gatcgtcgcg cagatcgtgc tcggcatcct ggcgagcctg atcgtcatgg
     1861 cgttctcgcg ctggcgggag ttccgtgccg accgcggcgg cgcgaagctc gccggccgcg
     1921 acaacatgat cgctgcgctc gatgcgctga agagcgcaca cgagcctctg ccgcagcagt
     1981 ttgccgcctt tggcatcgcc gacggctcgg tctcccgcgg cttccagaaa ctgtggatga
     2041 gccatccgcc gctggatgaa cgtatcgcgg ccctgcgcgc acttccgccg aacttcgcct
     2101 gatcgctcgc tgccgggcat cgctcctcaa tgcgtgagat gagtgcaact gagctggtgg
     2161 agcggctcgg tgcggacact gttctcacgg aagccgcggc catcgagccg tttctcgccg
     2221 atcatcgtgg cctgtatcgg gggcgtgcgc tggctgtcgt cctgccgcgc agcgtcgggc
     2281 aactctcaac gctcctcgcc ctgtgcaacg atcgaaggac ggccgtggtc gcccatggcg
     2341 gcaacacgag ttactgcggc ggggctaccc cggatgagtc ggggcgtcag atcgtggtat
     2401 cgctcaggca cctcaaccgc atccgcaacg tagaccccct caactattcg ctggtggccg
     2461 aggccggttg tgtgctgtcc caggtgcagc gcgcagccga tgaggccgag cgtttctttc
     2521 ccttgagcct gggttccgaa gggacctgtc agatcggcgg caatctttcg accaatgccg
     2581 gcggcacgag tgtgttgcgc tacggcatga tgcgcgacct ggtgctcggc ctggaggtcg
     2641 tactcgccga cggacgcctg ctcagcaccc tgaccacgct ccgcaaggac aacaccggct
     2701 acgacatcaa gtcgctgttt ctcggcgccg agggcacgct cggcatcatc acggcagcca
     2761 gtctgaagct gtttcccaag gtccgcgcga ccgcaacagc gcttgcggcc gtcaccagcg
     2821 tgcaggcggc cgtcgagctg ctggcgagac tgcgcgaaag cagcggggat cgtgtcagct
     2881 ccttcgagct gatcccaagg atcgcgatcg atctcaccac gcgtcacatt cccgcagtgc
     2941 tcgatccgct gggccggccg cacgagtggt acgtgctgtg cgagctcagc tcctctcgcg
     3001 ccgcggatgc gctcgagtcc cttctggagg aattcctcgc agccgccatg gaggacaggc
     3061 tcgtcctcga tgcggtgatg acccgcagtg agcgcgaacg ggcggcgttg tggaaactgc
     3121 gcgagacgat tcccgaagcg cagcgacgcg atggcgcgag cctcaagcat gacatctcgc
     3181 tgccggtcgc ctcccttgcg gcgttcgtcg cccgggcggc gcaatggatc gggactcacg
     3241 tgcccgatgg gcgcctggtg gcctacggcc acgtcggcga tggcaacctg catttcaact
     3301 tgaatcaggc tcctggagct tcacgcgaag cattcctcgg gcgcgaggcg caggtgcggc
     3361 gcgtgctcca cgatctggtg cgtgaattcg gcggcagttt cagtgccgag catggcatcg
     3421 ggcggctgaa ggtggaggag ctcgagcgct acgccccacc cgtggagctc gacctgatgc
     3481 attcggtcaa acgggccctc gatcccaacg gcattctcaa tccgggcaag gttctgcggg
     3541 cgcagctatg atgcggccgc ggcgcggggg cgctgcgcga cttgcacgga gattcataca
     3601 tggtcccagg ccggctgatc attgtggcag cgctcctcat cctgtccgcc tgcagcaaat
     3661 cgaacgggcc cgcggctgaa gcccctgcgc tcacgaagga cgcgagctcg gctggcgcta
     3721 gcgtcgaagg ggcgccgcag atcgcgaccg aggccgatgg atcgcacatc gagtatcgcc
     3781 tctatggcca cggtgagccg gcgatcgtcc tgatccacgg ctgggccacc gatgcaaact
     3841 actggaatgc ccagcttccc gtgctcaagc cccgctacag cgtggtggcg ctcgatctgg
     3901 ccggacacgg cgcctcgagc cgcaatcgca ccgactggtc catgggcaat tacggcgagg
     3961 acgtggcgag cgtcgtgcgc cggttgcctg cccaacacgt gatcctggtg ggccactcaa
     4021 tgggcggcac cgtggcgctg gaggctacac ggcgcattgg tgaccgggtc atcggcatca
     4081 tcgtcgtcga tgcgctcaag tcggttggat tgcctcccct gggacagcgc gagatcgaga
     4141 tccggctcgc tccatttcgc agcgacttcg tcggccagac acgcaagtac gtcaccgagt
     4201 ctctgttcga gaagggcgcc gatcccgtgc tggtgcagaa ggttgcttac gacatgtcgc
     4261 tcgagccacc cgaagtcgcg gtgccctcga tgcaggcgct gctgggaatg aatctcgctt
     4321 ccgtgctgcc cgacatccac gtaccggtga tggccatcaa ctccgatctg gcgcccaccg
     4381 acgaagcccg gattcgcaag tcgctgccgg acttcaaagc cgacgtgctg ccgcacacgg
     4441 gccacttcct catgatggaa gtgccgcagc agttcaaccc catcctgctg cgcgatatcg
     4501 agacgctcgt ggcaaaagcc ccgcactgag tccgaccgcg gcagcgcccg gttgcagcag
     4561 cacctggacc ggggaacgcg ccgctcgtcc gggatcctcc ggcaggcctt gcaggctgga
     4621 ccaaacctca tcggtgtccg ggtccagaga gccacacgag accttgagcc agcccagatc
     4681 cgcgctgatc tcgtcatcgt cgacctgagt ccacacggtg gcgagcggcg tcgtctcgct
     4741 ctgatcctgc acgagcagga tcagagcatc ccgatcgggc atgaacggcc agtcctgact
     4801 gtgcaaggtc agctcgagct tgcgcccgtc gggtgtcacg aagtgtccga tgccgccctg
     4861 atcgtgcaga tcgagtcgca cgagcggccg cagatccgaa cggcgtcccg tcgctgacca
     4921 ctccacccgg caggcaaaca gacgcgcatc attcagctcg aaccagttga agccgaccac
     4981 ttcctccaga acgtcctcgc tcggcttgga caggatgcgc cgccggtacc agtacaggtc
     5041 cggaccgatc agcagctggt agtccacata tcgattgctc ccctcgcgcg ggtcggtgca
     5101 atgccgtccc agcgtgccgc ccatgaactg atcggcctcg cgcttgagcc gcaggtcgca
     5161 gccggcgacc ggcgcgatgt cggtgcggcg cagactggcg acccgcgtgg ggagccggtc
     5221 cagatgcacc caggagtgtg ggtcccggaa ggcgaacaga tgaacctcga cccccacgtc
     5281 cggcggctcg accggctcga gctgcagcag cagctgtcgg cgcaccgtgt caggctcgtc
     5341 gtggagaaat tcctcgagat acaacacatg cgcgccgagc cagggaacgc tcaccggcgc
     5401 cacgatcgtg cgcacgcgcc gctccgcacc caaaccccag ggtgagacac tcgcatcagt
     5461 accgacgaag gtctgttcgc tcgaatcgcg aatccccggc cagatctgcg ccagcgcatc
     5521 gacgtcggat ttgccggaag gccctgcggt cgccagtgcc gccacagcca gcatcagcaa
     5581 catgcggcta ccaacctgtc aggtcttgcc cggccccggg gcatgcttca gccggacaag
     5641 gccctcctga gcggtactgg tgacgagccg gccatcccgg gcaaaaacgc ttgcccgcgc
     5701 aaacccccgc gcacccgagg cgctcggact ctccatggca tacagcaacc agtcgtccac
     5761 gcgcagagga cgatggaacc acatcgcatg atcgaggctc gccatgacga tcgggctcgt
     5821 gaggaaagag gccccgtgcg gcagcgtcgc ggtgtcgagc agaaagaagt cggagacgta
     5881 ggcaatcaaa cagcgatgca gctgctcgcc gtccggcaac gctcccacgg cacgaaacca
     5941 gatctgtcgc gccggtggcg ccttgcgcgg gcgcaggaag tcgatcggct gtaccggacg
     6001 gaattcgaac ggccggggct gctcgaagaa acgccgcagg cgttccggca tgcgtgcgag
     6061 cagctcggcc ggcaacaggc tcgagtccgc caggctttcc ggcgagggca ccgtgggcat
     6121 tgcgatctgg tgatcgagac ccggctcggt gacctggaac gaggcggcca tgttgaagat
     6181 ctgctcgcca tgctgaatcg ccaccacacg gcggttggaa aagctgtgtc cgtccaggct
     6241 gcgatccacc tggtacacga tcggcgcctg gggatcgcca cggcgcagga agtaggcgtg
     6301 cagcgaatgc gccaggcggc cctcgatggt gcgtgaggcg gccatcaggg cttgcccgag
     6361 gacctgcccg ccaaagacct gcgggctgcc gatgtcgcgg ctctcacccc ggaaaaggtt
     6421 gatctcgagc tgctcgagct cgagcagctt caacaggtct gcaagccggg ggtccatggg
     6481 tgcgcagcgc aggcctttca gccgaaattg aaggtgccgc gcatcgagga gttatgtccc
     6541 ggataggtgc acagatacat gtaggactct cccttgcgca gccccgccgt cgagaatgtc
     6601 acgctggtgc tctcaccgcc gcccacgacc cgcgtggcgg caatcacccg cttgtctccc
     6661 ggcgcgatgt agttgttctg cagaccggcg gccatgccgg cattcgccac ggcattgaga
     6721 tccgccgcct tcacgagcac ccagttgtgg cccatggcct ccttcggcag cttcccgacg
     6781 tgcctcaggg tgagctgcac ctgcgtgcac gctcctgaga gcgccagcag cttcctgtca
     6841 tattgcatca ggtcgttgcc ggtgatctcc agcttgcata catcaccggc cgcatgggcc
     6901 gcccgcgggc tcgccattgc aagcccaacc agcatcgccg ccgcaatgag ctctcgcctc
     6961 aaccgcatcg cccgctcctc atcggtattg ccctagcctg ccgcaatgct caatccggca
     7021 cgccgagccg ctgatgcatg atggggctcg tggtcgtgta ctgcaggaac tgtttctttt
     7081 gcggatacag atggtgctgg atggcgaatg cggcgagcgc cgcctcgtgg aagcccgaca
     7141 ggatcagctt cttcttaccg ggataagtgt tgatgtcacc caccgcgaac acacccggca
     7201 ggctcgtctg gaatttctcc gtatccaccc tgagcgcctt cttctccagc tcgagacccc
     7261 actgggcgat cgggccgagt ttcggatgca agccgaagaa caccagcagc tgttgcgcgg
     7321 gaagcgcgtg cacaaccccg tccggggcct tgatgttgac cccgcgcagc tcaccttcct
     7381 cgatgatcaa agagtgcggc agcgcctcga agcagcgcat ccggccaccg gccacgagct
     7441 cgcgcatctt cgcaaccgag gcgggcgccg cgcggaactc cggacggcgg tgcacgagtg
     7501 tcacgcttgc cgctttcgct gcaagctcca gcgtccagtc gagcgcggag tcaccgccac
     7561 cgaagatcac caggtgctgc ccattgagcg cactcgaggc cctgatgcga tagtgaatcg
     7621 tcttgccctc gaagctgtcg gcgctctcga ggctgattcg ccgcggctga aatgagccca
     7681 caccggcggc gatcacgacg gcgccggcat cgaactcggt accggccgag gtgacgaccc
     7741 ggaatcggcc atcatcgcgc cgcgtgaact cggtcacctc ctgccccagg tgaaactgtg
     7801 gatgaaacgg ctcgatctgc ttcagcagac gatcgaccag ctcctgggcg ccgcacaccg
     7861 gcagcgccgg gatgtcgtaa atgggcttgt caggatagag ctcggcgcac tgcccgccgg
     7921 ggcgcgcgag agagtcgatg acctgcgagg ggatgccgag cagccccagc tcgaagacct
     7981 gaaacaggcc gcagggccct gcgccgatga tgactacctc ggccgaggta ggcgaacggg
     8041 atgtctggct caagaaatct gttccgcttg gaccggcgcg tattgtagag ggtctgcgca
     8101 cgggccgcat ggccgcaccc ggggcgccac cgtagcgcgc ccggcgcgcg cgcgccggcg
     8161 gccgaacagc cgctcgcggg tgcagcccag gcggggttca ggcacaattt ggggcggcgc
     8221 cttgcgcctg cccaccacaa ggggaactgc cttgaattcc aactccgagc tgcaccagcg
     8281 ccgccagaat gccattccgc gcggcgtgtc gaactccctt gcggtctatg ccgaacgggc
     8341 ctccaacgcg gagctctggg atgtggaagg acggcgctac atcgacttcg cgagcggcat
     8401 cgccgtgctc aataccggac atgtgcatcc gaaagtcaag gccgcggtgg ctgcacagct
     8461 cgagaagttc acgcatacct gcttccaggt cactccctac gagagctatg tggcgctcgc
     8521 ggagcagctc aacgagatcg caccgggtcc gaccgccaag aagaccattt tcctgaccac
     8581 cggggccgag gcgatcgaga acgccatcaa gatcgcgcgc ttccatacga agcgctcggc
     8641 agtcatcgct ttctcgggag gctttcacgg gcgcaccctg gcgtgcatca gcctgaccgg
     8701 caaagtgcag ccctacaagg cgggcttcgg accgatgctg cccgagattt ttcacgtccc
     8761 ctacccgatg gcctatcacg gcgtcagcgt cgaagactcg ctggaggcct tgtcgcagtt
     8821 gttcaaggcc gacgtggacc ccgcacgggt ggcggccatc atcatcgaac cggttctcgg
     8881 cgaaggcggt ttttacgcgg ctcctgcgga gctgctgcgc aagctgcgcg ccttgtgcga
     8941 tcagcacgga atcctgttga tcgtcgatga gatccagacc ggcttcgggc gcacggggcg
     9001 gatgttcgcg atcgagtatg ccggcatcga gccggatttg atgacgatcg ccaagagcat
     9061 tgcaggcggc gtgccgctct cggcagtcat cggaaaggcc gagatcatgg actctcccgg
     9121 cccgggagga ctcggcggca cctatgccgg atcaccgctc gcctgcgctg cgggactggc
     9181 cgtgctggag gtcatccgcg aggagcagtt gctcaaccgc gcgctgcaca tcggtcattt
     9241 catgagctcg cgtttgaagg gcctgcaggt ccgctttccc tgcctgggtg acatccgcgc
     9301 gctcggcgcg atggtggcgg tcgagttggt tcgtaacaat gcggactcac cggatgcgga
     9361 gctgacccgc acactggtgc aggccgcagc gcgcaaggga ctgatcctgc tttcctgcgg
     9421 catttacggg aatgtgatcc gctttctcgc gccgctcacg attccggatg ccctgctgaa
     9481 ggagggattc cacctgttcg aacaggcact gcaggaggtc gcgagcacga ccgtcgccgc
     9541 gcctgccgcc tgaggaagtc gcccgcggct caggctcgtc cggcgttggc atccgtcggt
     9601 gtatggaagt actcgctctc ggcggcgagt tgctccatca ggcgtttgag ctccgccatc
     9661 cgaccttccg ccgtgctgat cggcatgcaa tcctggcagc gcggatcccc gcagggctgg
     9721 cgcgagtaca accagtactg cagtgctcct gcgagcagcc cgtcggcgat gtcccacagg
     9781 tcggcgtctt tgtctttgtc cgccagccga ttgccgaggt cgacggcttt cgtgaaggcc
     9841 gagtcgaagg tatcagcgat ctcggtcatg tgcccctcat ccccgtcatg ccgctagtat
     9901 agagcaacac gggcccggat cgggcttccc gcaggcccgc cggagcgccc ctcccgcgct
     9961 ccggagcgca gctgtaaacg gccgtgtttt ccacaatccg ccgtgcgacg gcccactgtt
    10021 acgcttctgc gatggccgcc acggaagtcg cccgggactg cccgcatcat gcagcgacgc
    10081 cgaaaaggcc gacaggtact gtagttctgt tggcctcggc ttatgtctta tgcatgaaac
    10141 gcccatcacg gtcgaggcca ccgagatcgc tgccacagcg gtctctactt cacaggcggc
    10201 acccagtccg tcgaggctgc gcctgggaat cggcgggcgc ctcgcgctgg gtctggctgc
    10261 gctggccgc
//
LOCUS       DASRHN010000014         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1157784, whole genome shotgun sequence.
ACCESSION   DASRHN010000014 DASRHN010000000
VERSION     DASRHN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157784"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..533
                     /locus_tag="VFB37_00490"
     CDS             69..533
                     /locus_tag="VFB37_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20946.1"
                     /translation="MGSQNESGQPGAILFAEFWKRASKIREVWKVPFDPGNPIHTPRG
                     LNLSDKVATEIARAIADAVEVLQREQLPRDTANGDALFALAGHEHIPLQGGDGSDGVS
                     NALDASFVSSVRGSFSTRGTVARQPGQARHLRVRSNPDVSRRKDVPANAGRS"
     gene            complement(812..1567)
                     /gene="istB"
                     /locus_tag="VFB37_00495"
     CDS             complement(812..1567)
                     /gene="istB"
                     /locus_tag="VFB37_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZO20947.1"
                     /translation="MLIQPTLDALNRLKLHGMALALSEQMTNTATQQLAFEERIALLL
                     EREVTHRENSRMGRLWKACQPKERGAVIEDIDYRGRGGLDRVQLASLASCEWIRQSQN
                     LLIHGATGSGKTYLACALAHQACRAGLSAWYVRAPRLFEELSLCHADGTFRKRLAAIA
                     KIQLIVVDDFAISPIGPRERNDLLELLDDRVGSRASILTSQLPIEHWHEYLNGDPTLA
                     DAIMDRLIHCSHKIHLHGKESMRERTSKKAAKG"
     gene            complement(1621..3165)
                     /gene="istA"
                     /locus_tag="VFB37_00500"
                     /pseudo
     CDS             complement(1621..3165)
                     /gene="istA"
                     /locus_tag="VFB37_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            3380..3763
                     /locus_tag="VFB37_00505"
     CDS             3380..3763
                     /locus_tag="VFB37_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HZO20948.1"
                     /translation="MVRKTHQMGGIGPMQGQATAFERYIQEDVPLARARYKNETRRLY
                     EVLNRRLRGREFLCGDYSIADIASFSWVKTHRWARIPVEGLDDLLAWIERISARPAVQ
                     RGLLIPPPAGSSERQVSMGANMTTL"
BASE COUNT          632 a         1153 c         1264 g          743 t
ORIGIN      
        1 ttgagggagg tgacgtcttc ccatagggtg accatcgatg tccgtccagg ctgcggcgtg
       61 ctgcaggctt gggatcgcaa aatgaatcgg gacagccggg ggcgatactg tttgcggagt
      121 tctggaagcg ggcttcgaag atccgggaag tctggaaagt gccattcgac ccgggcaatc
      181 cgattcacac cccgcgcggc ttgaatctct cggacaaagt ggccaccgaa atagcccgag
      241 cgattgccga cgctgtcgag gtactacaaa gggagcaatt gccacgcgat acagccaatg
      301 gtgatgccct cttcgcgctg gctgggcatg aacatatccc gttgcagggc ggggatggct
      361 ccgatggcgt atcgaacgca ctggatgcca gtttcgtatc cagcgtcagg gggtcatttt
      421 caacccgtgg caccgtagcc cgccagcccg gtcaggcgcg tcatcttcga gtccggagca
      481 atcctgatgt atctcgccga aaagacgtgc cggctaatgc cggcagatct tgacggccga
      541 tacgaggtga tccaatggct aatgttccag attgcgtatt ccggcgattg tgaccggccg
      601 ttccggccga acgtgaccgg gcattccgcc aggtcggcgc tgtgagggtt tattactccg
      661 atcggtcacg ttccgtcaac gctcgcccac gctctgctcg acactcttcg ctccccgttt
      721 cacggggaag ccgtcgggga gtctgaccgc ctggagggcg ggcagaaggg cctgcgcggc
      781 gtttcgacgg ggtcgcgacc tcatcatcgc ctcagccctt cgcagccttt ttgctggtgc
      841 gttcacgcat cgactccttg ccatgcaggt ggatcttgtg cgagcagtgg atcaggcgat
      901 ccatgatcgc gtcggcgagc gtggggtcgc cgttcaggta ctcatgccag tgctcgatcg
      961 gcagctgact ggtcaagatc gatgcccggg atccgacgcg atcatcgagg agttcgagca
     1021 ggtcgttgcg ttcgcggggt ccgatggggc tgatcgcaaa atcatcgacc acgatgagtt
     1081 ggatctttgc gatcgcggcg aggcgcttgc ggaaggtgcc atcggcgtgg cagaggctga
     1141 gctcctcgaa gagtcgcgga gcgcgcacgt accaggcaga gaggcccgca cggcacgcct
     1201 ggtgcgccag agcacaggcc aggtaagtct tgccgctgcc ggtcgcgccg tggatcaaga
     1261 gattctggct ctggcggatc cactcgcagg aggcgaggct ggccagctgt acgcggtcga
     1321 ggcccccgcg tccgcggtag tcgatgtctt cgatcactgc accgcgctct ttgggctggc
     1381 aggccttcca cagccgtccc atgcggctgt tttcccggtg ggtcacctcg cgttcgagca
     1441 gtagcgcgat gcgctcctcg aaggcgagct gctgcgtggc ggtgttcgtc atctgctcgc
     1501 tcagggcgag cgccatcccg tggagcttca ggcgattcag tgcatcgagg gtgggttgga
     1561 tgagcatgga tcatcatctc cttcggtagt ggacggcggg gccgtctggg tggtcgcacg
     1621 gaagtactgc gccccgcgca cgttggcatg cgagcgcgcg gcggtcggcg cggccgtgtc
     1681 cgcgccggga aagaggtccg ggtgttgatc gagcttggcc ttgaggatcg cctcgatgcg
     1741 cttgcgggtc ggggaaccga tggtcagtgc ccggcgacag gccgcttcca gtcgctgctc
     1801 gccgtaggtc ttggcgaggt tcaacaggcc caggcacgtc cgataaccct gctcgggatg
     1861 cggccgattg tcgaactgcc attgcaccac cgcgcgggtg cccgggccga tcttctggcc
     1921 ccagttcagc agccggcccg gcgtccactg catgtgccga cggtgcgact cgggcatgtg
     1981 ctcaggcaag gtggtgtgcg caccgcgctg gtaggagcgc acgtgcgcgg cgacgcggcg
     2041 gcccttgaac agacactcga tagcagacac cgtcatccgc acgcgcgtgt actcaccgac
     2101 cagcgggtgc ggcacgctgt agtagtgtcc gtcggcttcg acgtggtagt cgatgccgac
     2161 cttggctttc agccactcgg cgtattcgta gcgcctctcg ggcagcggtt tcatcgccgg
     2221 ccggtcgatc aactcgaagg ccttggcgcg gcaaccaggg agcttcttga acgctcggtg
     2281 attgagctca acgagcagcg ctgcgatcgc agcgttgagc tccggcagac agaagaactg
     2341 gcggttgcga aggcgcgcca ggatccagcg ctgcaccacg agcacgtggt tttctacgac
     2401 cgctttgtcc ttcggagatt tcggccgtgc cgcgataatc gcacacccgt aatgacgcgc
     2461 catgtcgccg taagtggagt tctcttccgg ctcgtagcga cacgccctgt tgatggcgct
     2521 cttcaagttg tcaggtgtta ggatctcggg cacgcatccg aggtgctcga acatccggat
     2581 gtgcgaggcg atccagtcgg gcagctgctg cgtccaggtg gcttccgcat aggtgtagtt
     2641 gctcgctccc atcgaggcga cgaagatctg cgctcgcagg atctccccgg tcacggaatt
     2701 gatgaccggg acggtgtcgc cgctatagtc gatgaagagc ttctcgccgg cgcggtgggt
     2761 ctggcgcatc gagcgggcga ggctgtcgtg gaagcgctgg tagtgccagc agaactggct
     2821 gtaccggtac gcccgctcgc cgtgactggc gtggtactcc tcccacagca gctgcagcgt
     2881 cacccctttg cgcttgagct cctggtgcac gtacgcatag tcggcgtgcg tgtattgctc
     2941 gcgctgcagg acttggggga acagcaaccg ctcgagggcg ctctcgtcca gctcctccgg
     3001 cggcggccag ccgaggccct tctggatggc ccgctgcacg taattgctca cggcgccctt
     3061 ggagaggccg gtcgccgcgg cgatccgctc gtgactcaat cgcgcctcga acttcaggcg
     3121 caggacttct ttgattttgc gcatggcact ccgcttggct ggcatcggct ctccccgaga
     3181 acaaaatcgg gcagcttagc cagcggtcag gggtccagcg cagacccggc gggatcattc
     3241 cgggggaacg tgaccgagga ttccgggatc ttgaccggcg attccgggcg caagcccaaa
     3301 atcggtcacc ttcgagcgga atgagtggtc acgttcggcc ggaacggccg gtcacgttca
     3361 gccggaatca gcggtcacga tggtccggaa aacgcaccag atgggaggca tcgggcccat
     3421 gcaaggtcag gcgaccgcgt tcgagcgcta tattcaagag gacgttccct tggcccgggc
     3481 ccgctacaag aatgagaccc gacgcctcta cgaagtgcta aaccgacgcc tgcgaggccg
     3541 tgagttcctc tgcggagact acagcattgc ggacatcgcc agcttctcat gggtaaagac
     3601 ccatcgttgg gcgcgcattc ccgtagaggg tctcgatgac ctgctggcgt ggatcgagcg
     3661 tatctccgcc cgtcccgcgg ttcagcgcgg cttgcttatt cctccgccgg ccggctcttc
     3721 ggagcggcag gtcagtatgg gcgctaacat gaccaccttg taaggcttgt ccggcggcgc
     3781 cggaactcac ct
//
LOCUS       DASRHN010000015         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1981590, whole genome shotgun sequence.
ACCESSION   DASRHN010000015 DASRHN010000000
VERSION     DASRHN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981590"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFB37_00510"
     CDS             complement(<1..427)
                     /locus_tag="VFB37_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HZO20949.1"
                     /translation="MATSAPEPQREFDLEPTDNARLANLCGPLDENLRLLESRLAVQI
                     RRRGDNFRILGAHAGSAEDTLRELFDLAKDEEVTPERVHLALQERESGPVVGDPQQHE
                     NAVRVPRGGIRARGTHQREYLAHIRNRDLTFGIGPAGTGK"
     gene            complement(436..1911)
                     /gene="miaB"
                     /locus_tag="VFB37_00515"
     CDS             complement(436..1911)
                     /gene="miaB"
                     /locus_tag="VFB37_00515"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZO20950.1"
                     /translation="MRYQSSLKEGLLPCAPASEALEFPIIPPMSRRYFIKTFGCQMNE
                     YDSARMADVLRAGGDLAATDNPAEADLLLMNTCSVREKAQEKVFSLLGEWRKLKAERP
                     HVLIGVGGCVASQEGEAITKRAPFVDLVFGPQTLHRLPDMIAELRRTGQSVVDISFPE
                     IEKFDRLPAPRAEGATAFVSIMEGCSKYCSFCIVPYTRGDEVSRPFDSVLEEIRQLAT
                     QGVREVTLLGQNVNAYSGAMSDGACVDLATLIHHVAAIPGIERIRFTTSHPLEFTDSL
                     IEAYANVPKLANHLHLPVQSGSDRILALMKRGYTALEFKDKIRRLRAVRPDISITSDF
                     IVGFPGETERDFNATLGLVRDAALDQSFSFLYSRRPGTPAASLPDEVEPAVKQERLAR
                     LQAQLETQASAISERMVGTVQRVLVERQARKNAHELAGRTENNRWVNFAGPPGSFESC
                     AARSGAGDESRVIRPATLINQFVDVVITELRAHTLRGRLLS"
     gene            complement(1962..2483)
                     /gene="ssb"
                     /locus_tag="VFB37_00520"
     CDS             complement(1962..2483)
                     /gene="ssb"
                     /locus_tag="VFB37_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZO20951.1"
                     /translation="MARGVNKVILVGNLGADPETRAMPSGMTVANLRIATSESWRDKQ
                     SGEQQERTEWHRVALFGRLGEVAAEYLKKGSQVYIEGSLRTRKWQDKQGNERFSTEIV
                     ASDMQMLGGRGGGGGMGGAGGGGMGGGSPGGGGGTGSGGSGGRGGDPYAEYSSSEAPS
                     GGTRDDFDDDIPF"
     gene            2613..>3548
                     /locus_tag="VFB37_00525"
     CDS             2613..>3548
                     /locus_tag="VFB37_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZO20952.1"
                     /translation="MQHIRVRGARTHNLKGIDLDLPRDRLIVLTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMDKPDVDTIEGLSPAIAIEQKATSHNPRSTVGTVT
                     EIYDYLRLLYARAGIPRCPDHLIDLTAQTVSQMVDQVLKLPEGTPVALLAPLISDRKG
                     EHAQVLEDIAAQGFVRVRIDGRIHEMEALPKLDPKRKHTIEAVVDRTRIRPDSGQRLA
                     ESFETALRLSNGLARLTFLDEPSREELVFSSQHACPVCGYSVPALEPKLFSFNNPAGA
                     CPTCDGLGTQEFFDPSRVVVNPDLSLASGAVRGWDR"
BASE COUNT          641 a         1172 c         1061 g          674 t
ORIGIN      
        1 tcttgccggt gcccgccgga ccgatgccaa atgtcaggtc acgattacgt atgtgcgcaa
       61 ggtactcgcg ctgatgtgtg ccccgcgcgc ggataccacc acggggcaca cgcacggcat
      121 tttcgtgttg ctgcggatct ccgacgaccg gtcccgactc gcgttcctgc agggcgagat
      181 gaaccctctc gggtgtcact tcctcgtcct tggccagatc gaagagctcc cgcagcgtgt
      241 cctcggcgct cccggcgtgc gcgccaagga tgcggaaatt gtcgccgcgg cggcggatct
      301 gaaccgcgag ccgcgactcg agcaggcgca gattctcatc cagcggacca cacagattcg
      361 cgagccgcgc gttgtcggtc ggctcgagat cgaattcccg ctgcggctcg ggcgccgaag
      421 tagccactgg agttttcagg acagcagcct gccgcgcaac gtgtgcgctc gaagttcggt
      481 aatcacgaca tccacgaact gattgatgag ggtagcgggt cggataacgc ggctctcgtc
      541 gccggctccc gacctcgccg cgcaagattc aaagctacct ggcggccccg cgaagttgac
      601 ccaccggttg ttttcggtcc tcccggcgag ctcgtgcgca tttttcctcg cctgccgctc
      661 caccagaacg cgttgcaccg taccgaccat cctttcactg atcgcactgg cctgcgtctc
      721 gagctgagcc tgcagtcggg caagcctttc ctgcttgacc gcgggctcga cttcgtccgg
      781 caacgacgcg gcgggggtac ccgggcgccg gctgtagagg aagctgaacg actggtcgag
      841 tgccgcgtcg cgcacgagcc caagcgtggc attgaaatca cgctcggtct cgccggggaa
      901 acctactatg aagtcggatg taatgctgat atccggccgc actgcccgca atctgcggat
      961 tttgtctttg aattcaaggg ccgtgtagcc ccgcttcatc aacgcgagga tccggtccga
     1021 gccgctctgc acaggcagat gaagatggtt cgcgagcttc gggacgttgg cataggcctc
     1081 gatcaggcta tccgtgaact cgagcggatg agaggtggtg aaccgtattc gctcgattcc
     1141 tggaatggcc gcgacgtggt ggatcagcgt cgccagatcg acgcatgcgc cgtccgacat
     1201 tgcgcctgaa taagcgttga cgttctgacc cagcagcgtg acctcccgga caccttgtgt
     1261 ggccagctgg cggatctctt ccaacaccga gtcaaatggg cgactgactt catcgccccg
     1321 cgtatacggg acgatgcaga agctgcagta cttgctgcac ccctccatga tcgaaacgaa
     1381 cgccgtagcg ccctcagcac gaggtgcggg caggcgatca aatttttcga tttcagggaa
     1441 actgatatcg acgaccgact gtcccgtccg acgcagctcg gcgatcatgt ccggcaggcg
     1501 atgcagcgtc tgggggccga acacgaggtc cacaaacggg gcccgcttcg tgatggcctc
     1561 accttcctgg ctggccacgc agccaccgac gccgatcagc acatgaggcc gctcggcttt
     1621 cagcttgcgc cactcaccca gcagcgagaa caccttctcc tgcgcctttt cgcgcaccga
     1681 gcaggtgttc atcagcagta ggtcagcctc cgctggatta tccgtggctg ccagatcgcc
     1741 gccggcccga agcacgtccg ccattcgggc agagtcgtac tcgttcattt ggcagccaaa
     1801 cgtcttgatg aaatagcgac gggacatagg gggaatgatg gggaattcta aggcctcgga
     1861 tgctggcgcg cacggcaaaa ggccctcttt gagggagctt tggtagcgca ccctagcaaa
     1921 atcaaagact tggggtggat gcgcgctacg ccaacctgcc cttagaaggg gatgtcgtcg
     1981 tcgaaatcgt cccgtgtacc acccgaggga gcttccgagc tcgagtattc cgcataggga
     2041 tcgcctccgc gcccccctga gccccccgat ccggtacccc cacctccacc cggagagcca
     2101 ccgcccatac cgccaccgcc ggcgccaccc atgccgccgc cgcccccgcg gccacccagc
     2161 atctgcatgt cgctggcgac gatctcagtc gagaaacgct cgttgccctg cttatcctgc
     2221 cacttgcggg tgcgcagact gccctcaatg tagacctgcg agcccttctt caggtactcg
     2281 gcggcaacct cacccagccg gccaaagagc gccacccggt gccactcggt gcgctcctgc
     2341 tgctcgccgc tctgcttgtc ccgccagctc tcgctggtgg cgatgcgcag attggcgacc
     2401 gtcattccgg agggcatcgc gcgggtctcg ggatcggcgc cgaggtttcc gaccaggatg
     2461 actttattga ctccacgcgc catacgaaat cctcaatagg tatgagcggc attctagcag
     2521 ccgccagcct gggatgagcc gccaataact gcgctgattg cccgaattcg cgcacggggt
     2581 agacttgagt gttttcctgg aaaagaccac tgatgcagca tattcgcgtt cgcggagcac
     2641 gcactcacaa tctcaaaggt atcgatctcg acctgcctcg cgaccggttg atcgtgctga
     2701 ccgggctgtc cggctccggt aagtcctcgc tggctttcga taccctgtac gccgaaggac
     2761 agcgccgcta cgtcgagtcg ctctccgcgt atgcgcgcca gttcctgtcg atgatggaca
     2821 agcctgacgt cgatacgatc gagggccttt cgccggcgat cgccatcgag cagaaggcga
     2881 cttcgcacaa cccacgctcg acagtcggta cggtcaccga gatctacgac tatctgcgcc
     2941 tgctctacgc acgcgcgggc attccgcgct gtcccgatca tctgatcgac ctgacggctc
     3001 agaccgtcag tcaaatggtt gatcaggtat tgaagctgcc ggagggcaca ccggtcgcgt
     3061 tgctcgcgcc gctgatcagc gatcgcaagg gcgagcatgc gcaggtgctg gaagacatcg
     3121 ccgcgcaggg gttcgtacgc gtacgcatcg acggccggat ccacgagatg gaagcgcttc
     3181 cgaaactcga tcccaagcgc aaacacacca tcgaggcggt ggtggatcgc acgcgcatcc
     3241 ggccggactc gggccagcgg ctcgccgaat cctttgagac cgcgctgcgc ctgtcgaatg
     3301 gactggcccg cctcaccttc ctcgatgagc ccagtcgcga ggagttggtt ttttccagcc
     3361 agcacgcctg cccggtctgt ggctacagcg tcccggcgct cgagccgaag ctgttctcgt
     3421 tcaacaatcc cgccggcgcc tgccccacct gcgatggact gggtacccag gaattcttcg
     3481 atcccagtcg cgtggtggtg aatcccgacc tgtcgctcgc gagcggcgcg gtgcgcggtt
     3541 gggatcgg
//
LOCUS       DASRHN010000016         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1818933, whole genome shotgun sequence.
ACCESSION   DASRHN010000016 DASRHN010000000
VERSION     DASRHN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818933"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFB37_00530"
     CDS             complement(<1..237)
                     /locus_tag="VFB37_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20953.1"
                     /translation="MAAIASRVDVHSQEFLDNQRALAELSADVRRQAARISQGGSPAA
                     VKKHKAAGKLTARERIRVLLDTGSPFLELSQLAAY"
     gene            302..1465
                     /locus_tag="VFB37_00535"
     CDS             302..1465
                     /locus_tag="VFB37_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HZO20954.1"
                     /translation="MAALDSDFGLDESLEEIRSQVRRFAAERIAPRAAEIDRSNEFPR
                     DLWPALGEQGLLGVTVPERFGGAGLGYLAHTLVMEEISRASGSVGLSYGAHSNLCVNQ
                     IRLNGTDAQREKYLPKLCSGEHVGALAMSEPGSGSDVVSMQLRAELRGDTYILNGRKM
                     WITNGPDADVFVIYAKTDVSAGPRGISAFIVERGTPGFSTAQKLDKLGMRGSGTCELV
                     FQDCAVPAVNLLGQLNRGAAVLMSGLDYERVVLAGGPLGLMAAALDLVLPYIRERKQF
                     GVPIGTFELMQAKVADMYAALNASRAYVYAVARACDAGKSKRRDAASCILFAAEHATR
                     AALEAIQALGGNGYTNDYAAGRLLRDAKLYEIGAGTQEIRRMLIGRELFEAGA"
     gene            1465..2196
                     /locus_tag="VFB37_00540"
     CDS             1465..2196
                     /locus_tag="VFB37_00540"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO20955.1"
                     /translation="MMEFDQIQIEPAYRKVAAAIGGRILDRSLREGDRLPPETELARQ
                     FGVNRSTVREALRELESSGLVTRRPGSKLMSVSRPQQLAIAETVTRGLVLHDVTFMEV
                     WEALTLLEPPMAEAAARARTTDDMVQVTSAAAQFAAGNAETADAAHGSAEFFRAVGRA
                     THNQILVLAQEPLLQLLEPSLRVMIDKVPQARSRITTAQRKIADVLQARDPEGARMWM
                     ARHIRDFRKGFEIAGIDLQHRVTAD"
BASE COUNT          487 a          803 c          850 g          451 t
ORIGIN      
        1 gtacgcagcc agctgcgaaa gctccaggaa ggggctgccc gtatccaaca gcacccggat
       61 gcgttcccgg gcggtgagct ttccggcggc cttgtgtttc ttcactgccg ccggcgagcc
      121 gccctgggaa atgcgcgcag cctggcggcg cacatcggcg ctcagctcag ccagcgcgcg
      181 ctggttatcg agaaattcct gggaatgcac atcgacacgg gacgcgattg cagccatatc
      241 ttgccatcat tgccggtttg tcggacaatc atacgatcgt actcaagggg aaggcaagct
      301 catggctgcc ctagacagtg atttcgggct cgatgagagt ctcgaggaga tacgctccca
      361 ggtgcggcgc ttcgccgctg agcgcatcgc gccgcgcgcc gccgaaatag accgcagcaa
      421 cgagttcccg cgcgacctgt ggcccgcgct cggagaacag ggactgctgg gtgtcaccgt
      481 gccggagcgc ttcggtggcg ccggtctcgg ctatctggcg cacaccctcg tcatggaaga
      541 aatctcacgc gcgtccggct cggtcgggct ctcttacggc gcccattcca acctgtgcgt
      601 gaaccagatc cgcctgaacg gcacggatgc gcagcgcgag aagtacctgc cgaagctgtg
      661 cagcggcgaa catgtcgggg ccctcgcgat gtctgagccg gggtccggtt ccgatgtcgt
      721 ctccatgcag ctgcgtgccg agctgcgcgg cgacacctac attctcaatg gccgcaagat
      781 gtggatcacc aacggaccgg acgcggatgt attcgtgatc tacgcgaaga ccgatgtctc
      841 agcgggtccg cgtggcatca gcgcattcat cgtcgagcgc ggcaccccgg gattttccac
      901 ggcgcagaag ctggacaagc tcggcatgcg cggttccgga acctgcgagc tggtgttcca
      961 ggattgtgcc gtgcccgcgg tcaacctgtt gggtcagttg aaccggggcg cggccgtgct
     1021 gatgagcggg ctcgactacg aacgcgtggt gctcgctggc gggccgctcg gcctgatggc
     1081 cgccgctctc gatctggttc taccgtacat acgcgagcgc aagcagttcg gtgtgcccat
     1141 cggtacgttc gagctcatgc aggcaaaggt ggctgacatg tacgcggcac tgaacgccag
     1201 ccgcgcctat gtttacgcgg tggcgcgcgc ttgcgacgct gggaaatcaa agcggcggga
     1261 tgcggcgagc tgcatcctgt ttgcagccga gcacgccaca cgggcggcgc tggaggcgat
     1321 ccaggcgctc ggcggcaatg gttataccaa cgattacgcg gcaggtcggc tgctgcgaga
     1381 tgcgaagcta tacgagatcg gggcgggaac gcaggagatc cgccggatgc tgatcggacg
     1441 tgagctgttc gaagcgggcg cctgatgatg gagtttgacc agatccagat cgagccggcc
     1501 taccgcaagg tggccgccgc catcggcggg cggattctcg atcgaagtct gcgtgaaggt
     1561 gaccgtttac cgccggagac agagctcgcg cgccagtttg gtgtcaaccg gtccaccgtg
     1621 cgcgaagcgt tgcgcgagct cgagagcagc ggtttggtca cgcgtcggcc tggctcgaag
     1681 ctgatgtcgg tgagtcgtcc acaacagctt gcgatcgcgg aaacggtgac gcgcggactc
     1741 gtgcttcacg atgtgacgtt catggaggtg tgggaagcat tgacgcttct cgagcctccc
     1801 atggccgagg ctgccgcgcg tgcgcgcact acagatgaca tggtacaggt taccagcgcg
     1861 gcggcgcagt ttgcggccgg gaatgccgag actgcggacg ccgcccacgg ctccgccgag
     1921 ttcttccgcg cggtcggacg cgcaactcac aaccagatcc tggtgctcgc gcaagagcca
     1981 ttgctacagc tgctcgagcc ctcgctgcgg gtgatgatcg acaaagtgcc gcaggcgcgc
     2041 tcacgtatta ccacagccca acggaagatt gcggatgtgc tgcaggcacg cgatcccgag
     2101 ggtgcgcgca tgtggatggc acgacatatc cgcgacttcc gcaaaggctt cgaaattgca
     2161 ggaatcgatt tacagcatcg cgtgactgcg gactgacagc tcagcgccaa ggtctgtcca
     2221 ccagcctgcc gccatgagcc cggaacaaaa aacgtgcgag gtgtcaccac ctcgcacgtt
     2281 ctaacgccgc gacgacgaat gagggggtca atcgccgctg cgaacgtggc ctcaaatgag
     2341 cactaaagcc ggagtcgtca tgctcagcgt gaggcgcacc gccagaccag ataaagcaag
     2401 agacatgcca gtgccttgcc cccctgatca gcctcccagc cctcgcgcgg cgcgcggaac
     2461 cgcaccataa cggggcactt tggaaaccta tggcgccaca gtgatccagt ccatcacgcg
     2521 gtcggcctga cgggcgagta gccgaccgaa caacggctgc agccgatcca actgctcggg
     2581 tggcatttgc t
//
LOCUS       DASRHN010000017         5485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1324582, whole genome shotgun sequence.
ACCESSION   DASRHN010000017 DASRHN010000000
VERSION     DASRHN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324582"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFB37_00545"
     CDS             complement(<1..671)
                     /locus_tag="VFB37_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HZO20956.1"
                     /translation="MSDLPRLMPTSAKAETFGGVTYHIDGELVPALTIDVTRMPVYFE
                     HHILLWKNPTVRIALKPMKGALKRMMAGLQVFITEASGNGLISFSRDGAGHIVPIHLA
                     QGNELHVREHQFLAATESVDYTFERVRGISNMMFGGTGFFIDKFHSHRADGILWLHGY
                     GNVFEKVLAAGESIDIEPGGWLYKDPAVQMRTIVDRLTSGLFGGMNFIVNRFTGPGRV
                     GIQSM"
     gene            813..1850
                     /locus_tag="VFB37_00550"
     CDS             813..1850
                     /locus_tag="VFB37_00550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZO20957.1"
                     /translation="MRYLENKERSTEILRLALPLMARQLAAFHPLSYALWYEHCAGMN
                     PDLSRVLDGRLAASSALTEMDVWRLYAQYIAARDIESFERLQLQLRNLLEDTAQSAAS
                     ASMQATEYGQSLETHAQALATPMDPDKIREVVEDMLESTQKMQVVTTELSAKLQASTK
                     EVNSLTESLRRAQSEALLDSLTGLKNRRGLERAVEDLQRETTGLMGTALLLADIDHFK
                     VVNDTYGHVLGDKVIRAVAHVLRSSIKGRDVAARLGGEEFAVLLPQTSLKGAAAVAEQ
                     IRGTVAQGRICRPDGNESIGQVTLSVGVAVAMPGDTLEALLERADAAMYTAKRAGRNQ
                     VSLAGDTPAAN"
     gene            complement(1892..2650)
                     /gene="fabG"
                     /locus_tag="VFB37_00555"
     CDS             complement(1892..2650)
                     /gene="fabG"
                     /locus_tag="VFB37_00555"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZO20958.1"
                     /translation="MPPEKSERASVPEVKRALVTGGSGGIGRAICARLARDGHHVYVH
                     AHQSVEAAKAVVEEIVGGGGRAQAVAFDVTDAGSTHAALTQILSEGPIQILVNNAGMH
                     DDAVFPGMSPEQWHGVIDTSVHGFFNVTQPLLMPMIRTRWGRIVNTSSVSALLGNRGQ
                     VNYAAAKGALNGATRSLALEVAGRGITVNAIAPGIIATTMSANAFDEAAIERLVPMKR
                     AGLPAEVAGLVSYLVSDDAGYVTAQVISISGGMA"
     gene            complement(2631..3140)
                     /locus_tag="VFB37_00560"
     CDS             complement(2631..3140)
                     /locus_tag="VFB37_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20959.1"
                     /translation="MTHLDRLWIEGHIPHHGGMCLLEEVLDWDASGVRCATTTHRALH
                     NPLRGFGRLGSACGVEYAAQAMAVHGALVASASGVAARRGLLASVRGVELHVERLDDV
                     DESLVASVERLASDDQTALYQFTLRAVESSALRVAGSGLPAQGRLLVSGRAAIAFSPG
                     EPNNATRKI"
     gene            complement(3137..3949)
                     /locus_tag="VFB37_00565"
     CDS             complement(3137..3949)
                     /locus_tag="VFB37_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HZO20960.1"
                     /translation="MPPLLARIEGVGLLGPGLLDWARSQDILGGRSPYRLQATVLPVP
                     EGLPPAERRRVGQIVKIALAVGTQAIRDADANPTDLASVFTSSGGDGQNCHEICRVLA
                     QEDRQLSPTRFHNSVHNAAAGYWSIANGATAPSNAVCAFDASFAAGLLEAITQVALEK
                     RPVLLVAYDAAYPSPLDEKRPIADAFGVALVLGPEREGQALSSQEPSRARIRVSLADA
                     AAECLTDPQLESLRASIPAARSLPLLIQLARREPGRVGVEYLDGRSLAIEVL"
     gene            complement(3956..5212)
                     /locus_tag="VFB37_00570"
     CDS             complement(3956..5212)
                     /locus_tag="VFB37_00570"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZO20961.1"
                     /translation="MSVQNSLTSVQNSATRAQATPASPLLLAHYTAVSCIGRGRGEIL
                     ASLREQRSGLAPCTFETVTLETCAGEVPAVDEERLPQALRDFDCRNNRLAQLGLRQDG
                     LAEAVARSAARWGPRRVGVFLGTSTSGILQTELAYRRLGATDGALPDDFHYATTHNSF
                     SVADFVRHALQLEGPAVVVSSACSSSAKVFGSASRMIEAGLIDAALVGGVDSLCLTTL
                     YGFHSLQLTSRLPCRPFDAERDGISISEAAAFALLERVPESVDSNSVLLLGVGESSDA
                     YHMSSPHPQGLGARAAMAAALQAASLEPGAIDYINLHGTGTVSNDAAESQAVQALFGT
                     STPGSSTKGATGHALGAAGALEAVICALSLQNDFMPGGANTRHPDPALQIEYLLRNRE
                     GPVRNVLSNSFGFGGTNCTLVLARAG"
     gene            complement(5209..>5485)
                     /locus_tag="VFB37_00575"
     CDS             complement(5209..>5485)
                     /locus_tag="VFB37_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20962.1"
                     /translation="PVLARLGLRLASWTRRGFDTVNRDPQVVLAKLTRNLSARDILLL
                     HDGHAARTPAGRPVILEVLPRLLEAFAAAELKPISLRSALDQSGAPS"
BASE COUNT          962 a         1828 c         1822 g          873 t
ORIGIN      
        1 tacatggact ggatgccgac acggccggga cccgtgaatc gattcacgat gaaattcata
       61 ccgccgaaga gaccgctcgt cagacgatcg acgatggtgc gcatctgcac ggccgggtcc
      121 ttgtacagcc agccgcccgg ctcgatgtcg atcgactccc cggccgcgag gaccttttca
      181 aacacgttgc cgtagccgtg cagccacaga atgccgtcgg cgcggtgact gtgaaacttg
      241 tcgatgaaga aaccggtgcc gccaaacatc atattggaga tcccgcggac gcgctcgaag
      301 gtgtaatcaa cactctcggt ggcggccagg aactgatgct cccgcacgtg cagctcattc
      361 ccctgggcga gatggatggg gacgatgtgc ccggcgccat cgcggctgaa ggagatcaga
      421 ccgttgccgc tcgcctcggt gatgaagacc tgcagtcccg ccatcatgcg cttcagcgcc
      481 cctttcatgg gcttcagggc aatgcgcact gtagggttct tccacagcag aatgtggtgc
      541 tcgaaataca ccggcattct cgtcacatcg atggtgagcg ccggcaccag ctcgccgtcg
      601 atgtgatagg tcacgccgcc gaaggtctcg gctttggcgc tggtgggcat caggcgcggg
      661 aggtcgctca tggggtcact ccgtaaggct cgttcccgga cccggcatat gtaaggcgcc
      721 gaggcggggc gcgggagggc tggccgcgtg tttcgcgagt gtggcaaagt accggccgag
      781 cgcaccagtg tcgagtcggg gtcgtgagcg aaatgcgtta tctcgagaac aaagagcgaa
      841 gcacggaaat cctgcgcctc gctctgccgc tgatggcgcg gcagctcgcc gcgtttcacc
      901 ccctcagcta tgcgctctgg tacgagcact gtgccggcat gaatcccgac ctcagccggg
      961 tgctcgatgg gcggctcgcc gcgagctccg ccctcaccga gatggatgtg tggcgcctgt
     1021 atgcccagta cattgcggcg cgggatatcg agtctttcga gcggttgcag ctgcagttgc
     1081 gcaaccttct ggaagacacg gcgcagagcg ccgcgagtgc cagcatgcag gcgaccgagt
     1141 acgggcagtc tctcgagacg cacgcgcagg cgctcgccac ccccatggat ccggacaaga
     1201 tccgcgaagt cgtcgaggac atgctcgaga gcacccagaa gatgcaggtg gtgaccaccg
     1261 agctctccgc gaagctgcag gcgagcacca aggaggtcaa ctctctgacg gaaagtctgc
     1321 ggcgcgccca gagcgaggcg ctgctcgact cgctcaccgg tctgaagaac cgccggggac
     1381 tggagcgggc ggtcgaggac ctgcagcgcg agaccaccgg actgatgggc acggcgctgt
     1441 tgctggccga catcgatcac ttcaaggtcg tgaacgatac ctacgggcac gtgctcggag
     1501 acaaggtgat ccgcgcggtg gcacatgtgc tgcgctccag tatcaagggt cgcgacgtgg
     1561 cggcccgcct gggcggagag gagttcgcgg tgttactgcc gcagacgagt ttgaaaggcg
     1621 ccgcggcggt tgccgagcag attcgaggca ccgtcgcgca gggacggatc tgccgtccgg
     1681 acggcaatga gtccatcggt caggtcaccc tgtcggtcgg ggtggcggtg gccatgcccg
     1741 gcgacacgct cgaagcattg ctcgagcggg cggatgcagc gatgtacacc gcaaagcggg
     1801 ccgggcgaaa ccaggtgagt ctcgccggcg acacgccggc cgccaactga cggccgccgg
     1861 gcctcggcgc cacgggtcgg gcgatcgtcg cctaggccat cccgccgctg atcgagatca
     1921 cctgcgcggt cacatagccc gcatcgtcgg agacgaggta ggacacgaga ccggccacct
     1981 ccgcgggcag tccggcgcgc ttcataggca cgagccgctc gatggcggcc tcatcgaatg
     2041 cgttggcgct catcgtggtg gcgatgattc ccggggcgat cgcgttcacc gtgatcccgc
     2101 ggcccgcgac ctcgagcgcc agcgaccggg tggccccgtt cagggcgccc ttggccgcgg
     2161 catagttcac ttggccgcga ttgccgagca gggcgctgac ggaggacgtg ttgacgattc
     2221 gcccccagcg cgtgcgaatc atcggcatca gtaagggctg cgtcacattg aagaacccat
     2281 gcaccgaagt gtcgatcacg ccatgccact gctcgggact catccccggg aagaccgcgt
     2341 catcgtgcat gcctgcgttg ttcaccagga tctggattgg accttcgctc agaatctgcg
     2401 tgagcgccgc gtgcgtcgac ccggcatccg tgacgtcgaa ggcgacagcc tgtgcgcgcc
     2461 cgccgccgcc cacgatttcc tcgacgaccg ccttcgccgc ctcgacactc tgatgcgcat
     2521 gcacatagac gtgatggcca tcacgagcca gccgggcgca gatcgcacgg ccgattccgc
     2581 cgctgccgcc cgtgacgagc gcgcgtttga cctcgggcac gctcgccctt tcagattttt
     2641 cgggtggcat tattcggctc tcctggactg aatgcgattg cggcacgccc acttaccaga
     2701 agtctcccct gggcaggcag acccgatccg gccacgcgca gcgcggagct ctccaccgca
     2761 cgcagcgtaa attgataaag cgcggtctga tcatcgctgg cgagccgttc gacactcgcc
     2821 acgagacttt cgtccacatc atccagccgt tcaacgtgta actcgactcc gcgaacactg
     2881 gcaagcagcc cgcgccgcgc cgccacgcct gacgctgaag cgaccaatgc gccatgaacc
     2941 gccatcgcct gggcggcata ttccacgccg caggccgagc cgagccggcc gaagccgcgc
     3001 agcgggttgt ggagcgcacg atgggtcgtg gtggcgcagc ggaccccgct cgcgtcccag
     3061 tccaacacct cctcgagcag gcacatgccg ccgtgatgcg ggatatggcc ttcgatccag
     3121 agacgatcca ggtgcgtcac aagacctcta tcgcaagact ccgcccatca aggtactcca
     3181 cacccacccg gcccggttcc cgccgcgcaa gctggatcag cagcggcaag ctgcgggccg
     3241 ccgggatcga ggcgcgcagg ctctccagct ggggatcagt caggcactcg gccgctgcat
     3301 cggcgagcga cacgcggatg cgagcccggg aaggctcctg ggaggataac gcctggcctt
     3361 cgcgctcggg ccccagcacg agcgccacac cgaaggcatc ggcgatgggc cgcttctcat
     3421 ccagcggtga gggataggcc gcatcgtagg ccaccagcag cactggccgt ttctcgagcg
     3481 cgacttgcgt gatggcctcc agcagtccgg cggcgaagct tgcatcgaag gcacatacgg
     3541 cgttcgaagg ggctgtcgcg ccgttggcaa tgctccagta gcccgcggcc gcgttgtgga
     3601 ccgaattgtg gaaccgcgtc ggcgagagct gccggtcttc ctgcgccagc accctgcaga
     3661 tctcgtggca gttctgccca tccccgcccg aggaggtgaa tacggaggcc aggtcggtgg
     3721 gattcgcgtc cgcgtcccgg atcgcctgcg tcccgactgc cagcgcgatc ttcacgatct
     3781 ggccgacccg ccggcgctcg gccgggggca ggccttcggg gacgggcagc acagtcgctt
     3841 gcaagcggta ggggctgcga cctcccagga tgtcctgcga gcgggcccaa tccagcaggc
     3901 cgggccccag cagacccact ccctcgatgc gtgccagcag cgggggcatg ctgcctcagc
     3961 ccgcgcgtgc cagcacgagc gtgcagttcg tgccgccaaa gccgaaggag ttgctcagca
     4021 cgtttcggac gggaccttcg cgattgcgaa gcaggtactc gatctgcaag gcgggatccg
     4081 ggtggcgggt gttggctccg cccggcatga aatcgttctg gagtgacagg gcacagatga
     4141 cggcctcgag cgcaccagcg gctcccaacg catgccccgt ggcacccttg gtcgagctgc
     4201 ccggcgtgct cgtgccaaac agcgcctgca ccgcctggct ctccgccgca tcgttgctga
     4261 ccgtgcccgt tccatgcagg ttgatgtagt cgatggcgcc cggttcgagg ctcgcggcct
     4321 gcagtgccgc cgccatcgcc gcacgcgctc ccagcccctg cgggtggggc gaggacatgt
     4381 ggtaggcatc gctcgactca ccgaccccga gcagcagcac ggagttggaa tcaaccgatt
     4441 ccggcacgcg ctcgagcagt gcgaaggccg cggcttcact gatcgagatc ccgtcgcgct
     4501 cagcatcgaa cgggcgacag ggtaagcgcg aagtcagctg cagagagtga aagccgtaaa
     4561 gtgtggtgag acacagcgag tccaccccac cgacgagcgc cgcatcgatc agacccgcct
     4621 cgatcatgcg actcgcactg ccaaacacct tggcgctcga ggagcaggcc gaggagacca
     4681 cgaccgccgg tccttcgagc tgcagcgcgt ggcgcacgaa gtctgcgacc gagaaggagt
     4741 tgtgcgtggt ggcgtagtgg aaatcatcgg ggagggcgcc gtcagtggcg cccagacggc
     4801 ggtaggccag ctccgtctgc aggatgccgg aggtgctggt gccgagaaat acgcccactc
     4861 gccgcggccc ccaacgggcg gcgctgcgcg ctaccgcttc ggcaagtccg tcctgccgca
     4921 gcccgagctg ggccagccga ttgttgcggc aatcgaaatc gcgtagcgcc tgcggcaggc
     4981 gctcctcatc cactgccggg acctctcccg cgcaggtttc caatgtcacc gtttcgaacg
     5041 tgcacggtgc gagcccgctg cgttgctcgc gcagcgaggc cagaatttcg cccctgccgc
     5101 ggccgatgca actcacggcg gtgtagtgcg ccaacagcag cggactcgcc ggagtcgcct
     5161 gcgcacgggt cgcagagttc tgcacactgg tgagagagtt ctgcacactc acgagggggc
     5221 cccgctctga tccagggccg agcgcaggct gatcggcttc agttcggctg cggcaaatgc
     5281 ctcgagcagc cggggcagca cttccaggat cacgggccgt ccggccggtg tgcgcgcggc
     5341 atggccatcg tgcagcagca ggatgtcccg cgccgagagg ttgcgggtga gcttggccag
     5401 caccacctgg gggtcacgat tcaccgtatc gaatccgcgc cgcgtccagc tcgccagccg
     5461 caagccgagc cgcgcgagca ccggc
//
LOCUS       DASRHN010000018         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1325148, whole genome shotgun sequence.
ACCESSION   DASRHN010000018 DASRHN010000000
VERSION     DASRHN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325148"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="paaA"
                     /locus_tag="VFB37_00580"
     CDS             <1..627
                     /gene="paaA"
                     /locus_tag="VFB37_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HZO20963.1"
                     /translation="LVDGAAIMNQIPICRCSYGPYARAMVRICKEESFHQRQGFEIML
                     SLARGTSAQHEMAQDALNRWWWPSLMMFGPSDAQSRHTEQSMRWKIKRFTNDELRQKF
                     IDITAPQAAFLGLSIPDPHLSWDEGSGHFRFGAIDWSEFQAVLKGNGPCNRERLEARR
                     KANDEGAWVREAAAAYALKQRARASAHASAEAVTAARAQFAATQGEHS"
     gene            639..935
                     /gene="paaB"
                     /locus_tag="VFB37_00585"
     CDS             639..935
                     /gene="paaB"
                     /locus_tag="VFB37_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HZO20964.1"
                     /translation="MAASPSDDWPLFEVFIRSRSGLEHKHVGSLHAADARMAVEHARD
                     VYTRRQEGVSIWVVRSTDISASDPEEADALFEPARDKVYRHPTFYSIPDEVKNL"
     gene            932..1684
                     /gene="paaC"
                     /locus_tag="VFB37_00590"
     CDS             932..1684
                     /gene="paaC"
                     /locus_tag="VFB37_00590"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HZO20965.1"
                     /translation="MSEDLFRFLLRLGDTNLVLAQRLGEWVGHAPALEEDLGLGNLAL
                     DHLGQARLLLTYAGEVEGQGRDEDALAFLRDAPEFCNLTLAEQPNGDFGRTLVRQWLI
                     DGWQRELYGALQGSTDSRLAAIAAKSLKETRYHYRFSAGWLIRLGDGTQESHARVQGA
                     LDELWRFTDEMFSPDEVDNRLAEVGVVPHLPALREGWLAAVDEVLKEATLKRPASMKY
                     PWHGKKGVHTEHLGHMLGEMQHLQRTYPGAQW"
     gene            1678..2175
                     /gene="paaD"
                     /locus_tag="VFB37_00595"
     CDS             1678..2175
                     /gene="paaD"
                     /locus_tag="VFB37_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HZO20966.1"
                     /translation="MVAPVIDGDAGHTFAVTPVRQAWQVLEAVPDPEIPVLSIVDLGI
                     VRFVRWSNEGHLHVGLTPTYSGCPATEVIRHSVAQALADAGFADAVLEEVISPPWTTD
                     WITAAGRRKLAAFGIAPPTTRQVACPRCGSQETQCISEFGSTPCKAHYRCNACLEPFD
                     HFKCL"
     gene            2179..>2432
                     /locus_tag="VFB37_00600"
     CDS             2179..>2432
                     /locus_tag="VFB37_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO20967.1"
                     /translation="MLRFHSLQVAGIQPNAEDAVSIALTGGAEVRGEYQGLPGQHIVL
                     RHEIAGQEVRRTYSLVSPPGAWPLQIVARIHPHGVMSGYLA"
BASE COUNT          442 a          756 c          807 g          427 t
ORIGIN      
        1 ctcgtcgacg gcgcggcgat catgaatcag atcccgatct gccgctgttc gtacgggccg
       61 tatgcgcgcg ccatggtacg catctgcaag gaggagagtt ttcaccagcg acagggtttc
      121 gagatcatgc tgtcgctcgc acgcgggacg agcgcccaac acgagatggc gcaggatgcc
      181 ctgaaccgct ggtggtggcc atcgctcatg atgttcggcc cgagcgacgc gcaatccaga
      241 cacaccgaac agtcgatgcg gtggaagatc aagcgcttca ccaacgatga gctgcggcag
      301 aagttcatcg atatcaccgc tccgcaggcc gccttcctgg gcctgtcgat tcccgatccg
      361 cacctgagct gggatgaggg cagcggccac tttcgtttcg gggccatcga ctggagtgag
      421 ttccaggcgg tgctcaaagg caacggcccc tgcaaccggg agcggctcga agcgcggcgc
      481 aaggcaaacg acgagggcgc ctgggtcagg gaggcggcag cagcctatgc cctgaagcag
      541 cgtgcccggg cttcggctca tgcatcggct gaggcagtga cggccgcgcg cgcgcaattc
      601 gcagcgacgc agggcgaaca ttcgtgaagg agatctccat ggcggcatcc ccatccgacg
      661 actggcctct gttcgaagtc ttcatccggt cgcggtcggg actggagcac aagcacgtcg
      721 gcagcctgca tgccgcggat gcgcgcatgg ccgttgaaca tgcgcgggac gtctacactc
      781 gcagacagga aggcgtgagc atctgggtag tccgctccac cgacatcagc gcgtccgatc
      841 ccgaggaggc cgacgcgcta ttcgagccag cgcgcgataa ggtgtatcgc cacccgacgt
      901 tctattcgat tccggatgag gtcaagaacc tgtgagcgag gaccttttcc gtttcctgct
      961 ccggctgggc gacaccaatc tcgtgttggc ccagcgcctg ggtgagtggg tcggtcacgc
     1021 acccgccctg gaggaggacc tcggactcgg aaacctggcg ctcgaccacc tgggtcaggc
     1081 ccggttgctg ttgacgtacg cgggcgaggt agagggacag ggccgcgatg aggacgccct
     1141 cgctttcctg cgggatgctc cggaattctg caatctgacc ttggccgagc aacccaacgg
     1201 cgacttcggc cggaccctcg tccggcagtg gctgatcgac ggttggcagc gggagctgta
     1261 tggcgcgctc cagggctcta cagacagccg gctcgcggca atcgccgcca agtcgctgaa
     1321 ggaaacccgc taccactacc gcttcagcgc aggctggctc atccgccttg gtgatggtac
     1381 ccaggagagt catgcgcgcg tgcaaggcgc cttggacgag ctgtggcgct tcaccgatga
     1441 gatgttctcg cccgatgagg tcgacaaccg gctcgcggaa gtcggcgttg tcccgcatct
     1501 gcctgcgttg cgcgaagggt ggttggcagc tgtcgatgaa gtgctgaaag aggcgactct
     1561 caagcgtcct gcgagcatga agtatccctg gcacgggaag aagggagtgc acacggagca
     1621 cctcggtcat atgttgggcg aaatgcaaca cctccagcgc acgtacccgg gggcgcagtg
     1681 gtagcgccag tcatcgacgg ggacgccggt cacacattcg ccgtgacgcc ggtccggcag
     1741 gcgtggcagg tgctggaggc ggtgcccgac cctgaaattc ctgtgctgag catcgtggac
     1801 ctgggaatcg tccgcttcgt ccgctggagt aacgaggggc atctgcacgt cggcctgaca
     1861 cccacctatt ccgggtgccc ggccaccgaa gtgatccgtc attcggttgc ccaagcgctg
     1921 gccgatgcag gcttcgcaga tgcggtcctg gaggaagtga tctcgccacc ctggaccacc
     1981 gactggatca ccgcagcagg gcggcgcaag ttggcggctt tcggcatcgc gccgcccacg
     2041 acccgacaag tcgcttgtcc ccgctgcggg agccaggaga cgcagtgcat cagcgagttc
     2101 ggctcgacgc cctgcaaggc gcactatcgg tgcaacgcct gcctggagcc gttcgatcat
     2161 ttcaagtgtc tgtagagcat gctgcgcttt cactccctgc aggttgccgg tattcagcca
     2221 aacgcggaag acgccgtatc gattgcgttg accggcggtg ccgaagttcg gggtgagtac
     2281 cagggcctcc ccggccagca catcgtgtta cgtcatgaga tagcggggca ggaggtgcgc
     2341 aggacgtatt ccctcgtcag tcctccgggc gcctggccac tgcagatcgt ggcccgaata
     2401 catccgcacg gagtcatgtc ggggtacctg gc
//
LOCUS       DASRHN010000019        13661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_995441, whole genome shotgun sequence.
ACCESSION   DASRHN010000019 DASRHN010000000
VERSION     DASRHN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995441"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFB37_00605"
     CDS             <1..543
                     /locus_tag="VFB37_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20968.1"
                     /translation="LGPGGRVRVLPDCSIPGAPNVFVIGDAAYLVDSRSGAQVPGVSQ
                     AALQMGRYVARVIREDMAAGTAAVGAPTEAGATLAPSRPAGRRLPIREAGFHYVDLGA
                     MATIGKSRAVVERGRLRFGGVLAWFAWLTLHVTVLIGFRNRIAVLLSWIYSYIFFRRG
                     SRLITGVDPGQSVHARWPPR"
     gene            545..2392
                     /locus_tag="VFB37_00610"
     CDS             545..2392
                     /locus_tag="VFB37_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO20969.1"
                     /translation="MPSRPPHPALDRTAGEARSLQGFFGVFRYSRRAIELVWSTNFAL
                     TLALAALTLIAGVLPAAVAYIGSLIVDAVVAAMRAEATGARRVIELVVLEGVLVAGVA
                     AAQRGLSLCQSLLRAQLGQRVNVMILEKALTLQLHHFEDSEFYDKLTRARREASSRPL
                     SLVMRTFGLVQNAISLISYGTLLARFSPWAVAVLLLAGLPAFVAETKFSGDAFRLFRW
                     RSPETRMQTYLETVIAREDYAKEVKLYGLGPRLLQRYRDIFGRLYDEDRALTIRRDAW
                     GFGLGLVATVALYAAYAWIALSTVRRAITLGQMTMYLALFRQGQAAVSAMLSAVGGMY
                     EDNLYLSTLYEYLETPVPSPEAGLPQGPEPEDGVRFEDVGFSYPGAEQPALEHIDLHL
                     RPGESLALVGENGSGKTTLIKLLTRLYPPTSGRILLDGLDLARWDESALRQRVGVIFQ
                     DFARYQMLVGENVGAGDEQYFEDEERWREAASKGQASDFIETLPAGYRTQLGKWFKEG
                     RELSGGQWQKIALSRAFMRTRADVLVLDEPTAAMDARAEADVFEHFRQLASGRITILI
                     SHRFSTVRMADRIAVLDSGRIIEYGSHEELMSLGGHYANLFALQARGYR"
     gene            complement(2462..2950)
                     /locus_tag="VFB37_00615"
     CDS             complement(2462..2950)
                     /locus_tag="VFB37_00615"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HZO20970.1"
                     /translation="MRHGPIPPRALWTLLVSAVCGALLAGCSSVPRQQRDQQQLEQYL
                     QYAGPPVDRITWLGHYYGWQSLSQTQLVVWTTPNEAYLITVVPPCTDLPFVQHIGLTS
                     TASTVSTRFDSVLVRHWRCQISEIRPIDYARLRKDLRARHQEDKAVPPHNSDEQDKDK
                     DQ"
     gene            complement(2963..5968)
                     /gene="treY"
                     /locus_tag="VFB37_00620"
     CDS             complement(2963..5968)
                     /gene="treY"
                     /locus_tag="VFB37_00620"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZO20971.1"
                     /translation="MPETESRPVPSAPVSAGQRSPGTTQSVPRARIPRATYRVQLNKS
                     FTFRDATAIVPYLARLGISHLYCSPYFRARAGSTHGYDVVDHNSFNPEIGDREDFERL
                     VATLRAHGMGHILDIVPNHVGIMGADNAWWLDVLENGQGSKYADYFDIEWNPGNAALR
                     GKVLVPVLGDVYGLVLERGELELRFEAEYGSFAVFYHEHRLPLDPGTYPRVLERALSG
                     HPNGELERLRQDFLALPQRQDPTEGEVLERHHAKEKLKRRLLELSAGNPALASALGTA
                     VGSFAGTPGEPASFDALHALLEAQAYRLASWRVASDEINYRRFFDVNDLAGLRVENEA
                     VFDETHRLVLELVRTGHLDGLRVDHPDGLRNPAQYFQRLQERIAALRGDVAVPDFGEA
                     GQDRPLPIYLVVEKITASFEHLPASWPVHGETGYHFANVVNRLLVDAATKARMDRVYQ
                     SFIGQFLEWPNVAYEAQHMILRRSLASELNVVANQLARIAQAERRTRDFTLNNIRQVL
                     AEVIACFPVYRTYVADRASESDCRYVDWAIASARRRRSVGEAPLFDFVRAALLLELTA
                     SSDAMRRRIRDFAMKFQQVTAPVTAKGVEDTSLYRFNRLTSLNEVGGEPDAYGSSVRA
                     FHADSQHRARFWPHEMLATSTHDTKRSEDVRARINVLSEMTQVWRKTIERWSRMNRSR
                     KRDVEGRPAPSENDEYLFYQTLVGSWPLQEPDPTELTTYRERIEGYMIKAAREAKSRT
                     SWADVNAEYEDALLQFIRGALEPRDGNLFLTDLGAFQKRAARFGLLNALSQVLCKLTA
                     PGVPDIYQGNEIWDFSLVDPDNRRPVDYEARRRMLENLIHATADPTTLLRSLLESLPD
                     GRAKLFLSWKALQLRCEHAQLFRDGEYIPLRVIGEHAANVCAFARQHRGNVALTIVPR
                     LYLRLLGEREEMPVGPAVWGDTMVELPKQFDTSIALRNCLDGTPVATEQTGSRSAIRL
                     SHALANFPVALLSAAAPSQPGGASTGSTL"
     gene            complement(5955..7793)
                     /gene="treZ"
                     /locus_tag="VFB37_00625"
     CDS             complement(5955..7793)
                     /gene="treZ"
                     /locus_tag="VFB37_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZO20972.1"
                     /translation="MSHRRHSMPFGAQIEADGRVRFRLWAPAAQRVELLLGEEARALE
                     MSAAASGWFELLTNTAQVGSLYRFRTDGRHEFPDPASRSNPGDVHGPSEVVDPLAFEW
                     QDEDWRGRPWQDAVIYELHVGTFSAAGTFAGVEAHLDHLEQLGVTVIELMPLADFPGQ
                     RGWGYDGVLLYAPESAYGTPQELKSLIDAAHRRGLCVMLDVVYNHFGPEGNYLGQYAP
                     QFFTERHHTPWGAAINFDGEGSRAVRDFFIHNALYWLEEYHFDGLRFDAVHAIIDDSP
                     QHILTEIAETIRARVGSGRHVHLVLENAANEAWLLGPPAGARTFDAQWDDDVHHCLHT
                     ILTGECDGYYADYVDHPHAKLCRSLAQGFVYQGQFSPHEGAARGEPSTHLPPTAFVLF
                     LQNHDQIGNRALGERLSHIVQSPEALRAATAVVLLAPSPPMLFMGEEWAAPEPFPYFC
                     DFEPELAARVREGRKREFAKFERFASPADTERLPDPTSAATFEAARLRWSRLSSGAHA
                     QWLDHYRRLLVVRQRDIVPLIPEIRSSSCIELESDGAFAVDWRLADGSLLHLLANLSD
                     QPLRLVGRAHGRMIFATHPNIRAALSRNELEPWSVTWLLEHGRAGN"
     gene            complement(7790..9964)
                     /gene="glgX"
                     /locus_tag="VFB37_00630"
     CDS             complement(7790..9964)
                     /gene="glgX"
                     /locus_tag="VFB37_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZO20973.1"
                     /translation="MDQQTTTARASAQHELRPIRAVWPGQPYPRGATWDGEGVNFSIF
                     SGNAEKVELCLFDPTGRHEVQRIALRERTDEIWHCYLPEARPGQLYGYRVHGPYDPAK
                     GQRFNPHKLLIEPYAKHLQGPLNWSDSHFGYRVGGAKADLSFDRRDSAAGTPKCRVVD
                     PAFSWADDRPPRVPWHDTVIYEAHVRGLTMRHPEVPPQLRGTYAGIATAPVVDHLLRL
                     GITTLELMPVHAFVDDRHLLEKGLRNYWGYNTIGFFAPELRYAASGRISEFKTMVKTL
                     HSAGIEVILDVVYNHTAEGNQLGPTLSFRGIDNAAYYRLVPDNPRYYMDFTGTGNTLN
                     LQHPRVLQLLMDSLRYWVLEMHVDGFRFDLASALARELFDVDRLGSFFDTIGQDPALS
                     QVKLIAEPWDIGSGGYQVGNFPPGWNEWNDKYRDTMRAYWKGDGGLIGDFARRFTGSS
                     DLYEASGRKPHASINFVTAHDGFTLLDLVSYNEKHNDANGEEGRDGSNDNRSWNCGVE
                     GPSEEPSVRSLRLHQRRNLLATLLLSQGVPMLLGGDELGHTHGGNNNAYCQDNAISWL
                     SWDLDPEQSDFVEFVARMIELRRRHPVFSRRRFLQDAAINAEGLKEIVWLTPEGTEMT
                     ETEWNQHFARCLGIYLAGAAIERRDRRGKAVTDNNFMLLFNAHHEEIPFVLPQSLSAK
                     AWWTVLDTSSASSPFEQRRHEAGAHYPLQGRSLALLRETAYR"
     gene            complement(9980..13279)
                     /gene="treS"
                     /locus_tag="VFB37_00635"
     CDS             complement(9980..13279)
                     /gene="treS"
                     /locus_tag="VFB37_00635"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZO20974.1"
                     /translation="MSAQPELPQPAGDPLWYKDAVIYEVHVRAFFDSNDDGVGDFGGL
                     TRKLDYIQDLGVNTIWLLPFYPSPGKDDGYDISDYHNIHAHYGTRGDFRMFVQEAHRR
                     GLRVITELVINHTSDAHPWFQAARRAPAGSPKRDYYVWSDSPDRYAGTRVIFRDTETS
                     NWALDPLAKTYYWHRFFSHQPDLNFDNPHVVRAVIHIMEYWLDQGVDGLRLDAIPYLV
                     EREGTSNENLRETHELIKRIRAVINEHYADRMLLAEANQWPEDVRDYFGDGDECHMAY
                     HFPLMPRMYMAIAQEDRHPIVEIMEQTPDIPDTCQWAIFLRNHDELTLEMVTSRERDY
                     MYRMYAADPRARLNLGIRRRLAPLMENDPERIKLMNSLLLSMPGAPIIYYGDEIGMGD
                     NFFLGDRNGVRTPMQWSPDRNAGFSRADPQQLYLPPIMDPIYGYEAVNVEAQTRDRSS
                     LLNWMKRMLQVRKTSQAFGRGTLRFIRPGNRKVLVYLREYGPDSILCVVNLARSAQPV
                     EVDLADFKGTVPIELIGRTPFPPVGDLPYLLTLPGYGFYWFQLSRDQQPPSWHDERLP
                     REDLPVLVLFDGWTSFFPERVANWRSGLATKLREQLETRVAPKYIASQRWYAGKGAPI
                     VRARLGDNGEWDTPLGRWLAGVFEVESSAGKALYFLPFAIAYEDTEESRWQKLQPGAI
                     ARVRRQATVGVLADATTDENFCRAVVEAIGQGARIRTERGEVHLSATAVYSQLAGDQS
                     APLSVTPTAGQSSNTTVRVGDQFFLKIYRRLQAGINPELEIGRYLTEVVRFPSIVPVA
                     GAAEYRNADGNTVTLALLQAFVTNQGDGWDYTVNYLSRFLEERRTEVALREDVHGVYL
                     ELMKTLATRTAELHRALAAPTSDPAFAPEPLRAEDIATWRLNARREAEATLEQLAKPP
                     LPIPQTLLPDVEAVLARRAALLKRIDSLGSVAPQGLKIRPHGDYHLGQVLVKRNDFII
                     VDFEGEPGRSLAERRLKQSPLRDVAGMLRSFSYARHAAVLRGMLHTPDDVGRWEPMLE
                     KWESETRRVFLTTYDAIASAAGLYRSLPEVRPLLELFELEKALYEARYELGNRPDWAS
                     IPLRSLLAFTG"
     gene            complement(13292..>13661)
                     /locus_tag="VFB37_00640"
     CDS             complement(13292..>13661)
                     /locus_tag="VFB37_00640"
                     /inference="COORDINATES: protein motif:HMM:NF044853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20975.1"
                     /translation="ARVNRIRRENPPLQSDRGLKFHATDSDMLIAYSKTGESGADSVL
                     VVVNLDPHNPQSGWLQLDLPTFGLPASGSYQVHDLLSGARFLWQGARNFVSLDPGRAP
                     AHVFRIRRRVRSEKDFDYFM"
BASE COUNT         2341 a         4314 c         4571 g         2435 t
ORIGIN      
        1 cttgggcccg gcggccgtgt cagagtgctg cccgactgct cgattcccgg agcgcccaac
       61 gtgtttgtca tcggcgacgc cgcctatctg gtcgattccc gctccggcgc gcaggtcccc
      121 ggggtttctc aggcagcgtt gcagatgggg cggtacgtcg cgcgggtcat ccgcgaggac
      181 atggctgcgg gcacagcagc cgtcggcgcg cccactgagg cgggggcgac actcgcacca
      241 agccgacctg caggccgcag gctgccgata cgcgaagccg gatttcatta cgtggacctg
      301 ggcgccatgg ccaccatcgg caagtcgcgc gccgtggtcg agcggggtcg tttgcgtttc
      361 ggcggtgtgc tcgcctggtt cgcctggctg actttgcacg tgaccgtgct gatcggcttc
      421 cgcaatcgga tcgccgtgct cctgtcgtgg atctacagct atatcttctt tcggcggggc
      481 tcacgcctga tcacgggcgt ggatcccggt cagagcgtgc acgcgcgctg gccgccccgc
      541 tgagatgccg agcaggccgc cccatcctgc gctcgacagg accgccggcg aggcgcgcag
      601 cctgcaaggc ttctttggag tcttccgcta cagtcgccgc gccatcgagc tcgtctggag
      661 cacaaacttc gctttgacgc tggcccttgc agcgctgacc cttatcgcag gtgtgctgcc
      721 ggctgcggtc gcctacatcg gctcgttgat cgtcgatgcc gtcgtggctg caatgcgcgc
      781 cgaggcaacg ggtgcgaggc gtgtgatcga gctggtcgtg ctcgaaggtg tgctggtcgc
      841 tggcgtcgct gccgctcagc gcggcctctc cctgtgccaa tccctgctgc gcgctcagct
      901 tggccagcgg gtcaatgtca tgattctcga gaaggcgctg acgctgcagt tgcatcactt
      961 cgaggactcc gagttctacg acaagctgac ccgtgcgcgc cgcgaagcct ccagccgacc
     1021 gttgagtctg gtgatgcgga cgttcggcct ggtccagaac gcgatctccc tcatcagcta
     1081 cggcacgctg ctcgcgcggt tctcaccctg ggcggttgcg gtgctgctgc ttgccggact
     1141 gcccgccttc gttgccgaaa cgaaattctc gggcgatgcc ttccgcctgt tccggtggcg
     1201 ctcgcccgag acgcgcatgc agacctatct ggagacggtg atcgcgcgcg aggactatgc
     1261 caaagaggtc aagctctatg gcctgggtcc ccggctcctg caacgctacc gcgacatctt
     1321 tgggcgactc tacgatgagg atcgcgccct gacgatccgg cgggatgcct ggggattcgg
     1381 actgggactg gtggcgacgg ttgcgcttta tgcagcgtac gcctggattg cgctcagcac
     1441 ggtgcgccgc gcgatcacgc tggggcagat gaccatgtat ctggccctgt tccggcaggg
     1501 gcaagcggcg gtctcggcca tgctgtcggc ggtcggcggc atgtacgagg acaatctgta
     1561 tctatccacc ctgtatgagt atctggaaac tccagtgcca tcgcccgagg cgggactccc
     1621 acagggcccc gagcctgagg atggggtcag gttcgaggat gtcggcttca gctacccggg
     1681 cgccgaacag ccggctctgg agcacatcga cctgcacctg cgccccggag aaagcctggc
     1741 gctcgtcggt gagaacggct cgggcaaaac gacgctcatc aagctgctga cccggctgta
     1801 tccaccgaca tccggccgca tcctgctcga tgggctcgac cttgcacgat gggacgagag
     1861 cgccttgcgg cagcgggtgg gcgtcatttt ccaggacttc gcccgctatc agatgctcgt
     1921 gggcgagaac gtcggcgcgg gcgacgagca gtatttcgag gatgaggagc gctggcgcga
     1981 agcggccagc aagggccagg cgagcgattt catcgagacc ttgccggccg gctaccgtac
     2041 ccagctcggc aaatggttca aggaggggcg ggagctctcc ggcgggcaat ggcagaagat
     2101 cgccctgtca cgcgccttca tgcgcacgcg cgccgatgtg ctggtgctgg atgagccgac
     2161 cgccgccatg gatgcccggg ccgaggcgga cgtcttcgag cacttccgtc agctggccag
     2221 cggtcggatc accatcctga tctcgcatcg cttctccacc gttcgcatgg cggaccggat
     2281 cgcggtgctc gattccgggc gcatcatcga gtacggcagt cacgaggagc tgatgagtct
     2341 gggtgggcac tacgccaatc tgtttgcact gcaggctcgc ggctatcgct gaggctcaac
     2401 cccgcggcag ccgaggcact cgcggctgcg caaacggcgc gcgccgagcc tgcggtgcag
     2461 gctactgatc cttgtccttg tcctgctcgt ccgagttatg cggcggcacc gctttgtcct
     2521 cctggtgccg ggcccgcaaa tccttccgca gccgggcata gtcgatcggt cggatctccg
     2581 agatctgaca tcgccaatgc cgcaccaata cactgtcgaa gcgcgtgctg acggtgctcg
     2641 ccgtcgaggt gaggccgatg tgctgcacga aaggcagatc ggtgcagggc ggtaccacgg
     2701 tgatcagata ggcctcattc ggtgtcgtcc agaccaccag ctgcgtctga ctgagggatt
     2761 gccagccgta gtagtgaccc agccaggtga tgcggtctac cggggggccg gcatactgaa
     2821 ggtattgctc gagctgctgc tggtcgcgct gctggcgcgg gactgaggag cagcctgcca
     2881 gcagcgcacc gcataccgcg ctgaccagga gtgtccagag cgctcgagga gggatggggc
     2941 catgtctcat ggcagctcct tgctagagag tggatccggt acttgcgcca ccgggctgtg
     3001 acggtgcggc ggcgctgagc aaggccacgg gaaagttcgc cagggcgtgt gagaggcgaa
     3061 tggcgcttcg tgagcctgtc tgttcggtcg caaccggcgt gccatcgagg caattgcgca
     3121 gtgcgatcga tgtatcgaat tgtttcggca gctcgaccat cgtgtcaccc cagaccgccg
     3181 gacccaccgg catctcctcg cgctcgccga gcagccgcag atacaggcgg ggaacgatgg
     3241 tgagcgccac gttcccgcgg tgctggcgcg cgaacgcaca aacattcgca gcgtgttccc
     3301 cgatcacgcg caacgggatg tactcgccgt cgcggaacaa ctgggcgtgc tcgcagcgca
     3361 gctgcagggc cttccagctc aggaacagct tcgcccgacc atcgggcagg ctctcgagca
     3421 gggaacgcag caaagtcgtc gggtcggctg ttgcgtggat gagattttcg agcatgcgcc
     3481 ggcgcgcctc gtagtccact ggccggcggt tgtccgggtc caccagcgag aagtcccaga
     3541 tctcgtttcc ctgatagatg tccggaaccc cgggcgcggt caacttgcat aacacttgtg
     3601 acagggcatt cagaaggccg aagcgggctg cgcgtttctg gaaggccccg aggtcggtca
     3661 gaaaaagatt gccgtcccgg ggctccagtg cgccgcgaat gaactgcagc aacgcatctt
     3721 cgtactccgc gttgacgtca gcccagctcg tgcggctctt ggcttcccgg gctgccttga
     3781 tcatgtagcc ttcgatgcgt tcccggtacg tggtgagctc ggttggatcc ggctcctgca
     3841 ggggccagct tccaaccaga gtctgataga acaggtactc gtcgttctcc gatggcgcgg
     3901 ggcggccttc cacatcccgt ttgcgcgagc ggttcatgcg gctccagcgc tcgatcgtct
     3961 tgcgccagac ctgcgtcatc tccgacagaa cattgattct cgcgcggaca tcctcggaac
     4021 gcttggtgtc gtgcgtcgag gtggccagca tctcgtgcgg ccagaaccgt gcgcggtgct
     4081 gcgagtcggc gtgaaaggcc cgcacactcg atccgtacgc atcgggctca cccccgactt
     4141 cgttgagtga agtcagacgg ttgaagcgat agagggaggt gtcttcgact cctttggctg
     4201 tgaccggtgc agtgacctgc tggaacttca tggcgaaatc ccggatgcgg cgccgcatcg
     4261 catccgagga tgcggtgagc tcgagcagca acgccgcccg cacgaagtcg aacagcggcg
     4321 cctccccgac cgagcgccgg cgacgcgcgg aggcgatcgc ccagtccacg tagcggcagt
     4381 cgctctcgga agcccggtcg gccacatagg tccggtatac cggaaagcag gcgatgacct
     4441 ctgccaggac ctgccggatg ttgttgaggg tgaaatcgcg ggtcctgcgc tcggcctgcg
     4501 cgatccgggc aagctggttg gcgacgacat tcagttccga agccagcgag cggcgcagga
     4561 tcatgtgctg tgcttcataa gcgacgttcg gccattccag gaactgaccg atgaacgatt
     4621 gatagacgcg gtccatgcgc gccttcgtgg ctgcatccac cagcaggcgg ttgaccacgt
     4681 tcgcgaagtg atagccggtt tcgccgtgca cgggccagga ggccgggaga tgctcgaagg
     4741 acgccgtgat cttctcgacc accagataga tcggcagcgg ccgatcctga ccggcctcac
     4801 cgaagtcagg taccgctaca tcaccgcgca gcgcagcgat gcgctcctgc aggcgctgaa
     4861 agtactgcgc cggattgcgc aggccgtcgg gatggtccac ccgcagcccg tcgaggtggc
     4921 cggtccggac gagctcgagc acgaggcgat gggtctcatc gaatacggcc tcattctcca
     4981 ctcgcaggcc ggccagatca ttcacatcga agaatcgccg gtagttgatc tcatccgatg
     5041 ccactcgcca ggaggcgagc cggtaagcct gcgcctcgag tagcgcatgc agggcatcga
     5101 agctggcagg ctcgccgggg gtcccggcga agctgccgac ggccgtgccg agggcgctgg
     5161 cgagcgcggg attgccggcg ctcagctcca acagccgccg tttcaacttc tccttggcgt
     5221 ggtggcgctc gagcacctcg ccctcggtcg ggtcctggcg ctgcggcagc gcgaggaaat
     5281 cttgccggag ccgctcgagc tcaccattgg ggtgcccaga gagtgcacgc tcgagcaccc
     5341 gcgggtaggt ccctggatcg agcggcaggc ggtgctcgtg atagaacacg gcgaaggagc
     5401 cgtactcggc ctcgaatcgc agctcgagct cgcctcgctc gagcacgagg ccgtacacat
     5461 caccgagcac cggtaccagg accttgccgc gcagggcggc gttgcccgga ttccattcga
     5521 tgtcgaaata atcggcgtac ttcgatccct gcccgttctc gagcacatcg agccaccacg
     5581 cattgtcagc gcccatgatg ccgacgtggt tgggcacgat gtcgaggatg tgccccatgc
     5641 catgcgcccg cagtgtcgcg accagtcgct cgaagtcctc gcgatcgccg atttcggggt
     5701 tgaaggagtt atggtcaacg acgtcatagc cgtgcgtact gcccgcgcgc gcgcgaaagt
     5761 aaggagagca atacaggtga ctgatgccca accgggccag gtagggcacg atggcagtcg
     5821 cgtcgcgaaa agtgaacgac ttgttgagct ggacacgata ggttgcccgc gggatgcggg
     5881 cgcgcggcac gctctgggtg gtgcccggcg agcgctgccc ggcagagacc ggcgcgctcg
     5941 gaacgggcct gctttcagtt tccggcacga ccgtgctcca gcaaccaggt gacgctccag
     6001 ggctcgagct cgttccggga gagggcggcg cgaatgttgg gatgggttgc gaagatcatg
     6061 cggccgtggg cacggccgac gagccgaagc ggttgatcgc tcaggttcgc cagcagatgc
     6121 agcagcgagc cgtcggcgag cctccagtcc acggcgaagg cgccgtcgga ctcgagctcg
     6181 atgcaactcg aggagcggat ctccgggatg agcggcacga tgtcacgctg acgcacgacc
     6241 agcagacggc gatagtgatc cagccattgt gcgtgagcgc cgctcgagag ccggctccag
     6301 cgcaggcggg cggcctcgaa tgtcgcggca ctggtgggat cgggcagccg ctctgtgtcc
     6361 gctgggctgg cgaaccgctc gaatttcgcg aactcgcgct tgcggccctc ccgcacccgg
     6421 gcggcgagct ccggctcgaa gtcgcagaag taggggaagg gctcgggggc cgcccattcc
     6481 tcccccatga acaacatcgg cggcgagggt gcgagcagca caacggcagt ggccgcgcgt
     6541 aaggcttccg ggctctgcac gatgtgcgag agccgctcgc ccagggcccg gttgccgatc
     6601 tgatcgtgat tctgcaggaa gagtacgaac gccgtgggtg gcagatgggt gctcggctcg
     6661 ccccgcgcgg cgccctcgtg gggcgagaac tggccctgat agacaaatcc ctgcgccagg
     6721 ctgcggcaga gcttggcgtg gggatgatcg acgtagtcag cgtagtagcc gtcgcactcc
     6781 cccgtcagaa tcgtatgcag gcagtggtgc acatcatcgt cccattgcgc atcgaatgtg
     6841 cgtgcgcctg ccgggggacc gagaagccac gcttcattgg cggcgttctc tagcaccagg
     6901 tgaacatgac gtcctgagcc cacccgggcg cgaatcgtct cggcgatctc ggtgaggatg
     6961 tgttggggac tgtcatcgat gattgcgtgc acggcatcga aacgaagccc atcgaagtga
     7021 tattcctcga gccagtacag cgcgttgtgg atgaagaaat cgcgcactgc gcggctgcct
     7081 tcaccatcga agttgatggc cgcgccccac ggggtgtgat ggcgctcggt gaagaactgc
     7141 ggtgcgtact gcccgagata gttgccttcc ggaccgaaat ggttgtaaac gacatcgagc
     7201 atgacgcaca ggccccggcg atgagcggcg tcgatcaggg atttcagctc ctgcggcgta
     7261 ccgtaggccg actcgggcgc atagagcagg acgccgtcat acccccagcc gcgttggccg
     7321 ggaaaatcgg caagcggcat cagctcgatc acagtgacgc ccagctgttc cagatgatcg
     7381 agatgggctt cgacgcctgc aaaggtcccc gcggcgctga acgtccccac gtgcagctca
     7441 tagatcaccg cgtcctgcca cggacggccg cgccagtcct catcctgcca ctcgaaggcg
     7501 agcggatcga ccacctcgct cgggccatgc acatcgccgg gattgctgcg cgaggcgggg
     7561 tcgggaaact catgccgccc gtcggtgcga aacctgtaca gcgagcccac ctgcgcagtg
     7621 ttggtcagga gctcgaacca gccgctggcc gcggcgctca tctcgagcgc tcgagcctcc
     7681 tcgcccagca gcagctccac gcgttgtgcc gccggagccc agagccggaa acggacacgg
     7741 ccgtcagcct cgatctgcgc cccgaagggc atgctgtgac gtcgatgact caccggtagg
     7801 cggtctctcg cagcagcgcg agcgagcgac cctgcagcgg gtagtgcgct ccggcctcat
     7861 ggcggcgctg ctcgaaggga ctgcttgcgg aggaggtgtc cagcacggtc caccaggcct
     7921 tggccgacag ggactgaggc aggacgaagg ggatctcctc gtggtgtgcg ttgaagagca
     7981 gcatgaagtt gttgtcggtg accgccttgc cgcggcggtc gcgccgctcg atcgccgcgc
     8041 ccgcgagata gattcccagg cagcgggcga agtgctggtt ccactccgtt tcggtcatct
     8101 cggtgccttc cggagtgagc cagacgatct ccttcaggcc ttcggcgttg atggccgcgt
     8161 cctgcaggaa gcggcgccgg ctgaacaccg gatggcggcg gcgcagctcg atcatgcggg
     8221 caacgaactc cacgaagtcc gattgctcgg ggtccagatc ccaggaaagc cacgagatgg
     8281 cattgtcctg gcagtacgcg ttgttgttgc cgccatgggt gtgtccgagc tcatcgccgc
     8341 ccagcagcat gggcacgccc tgcgacagca gcagggtggc taacaggttg cggcgctggt
     8401 gcagccgcag gctgcgcacg ctgggctcct cgctcggccc ctcgacgccg cagttccagg
     8461 agcgattgtc attggagccg tcgcgcccct cttccccgtt cgcatcgttg tgcttctcgt
     8521 tgtagctcac gaggtcgagc agagtgaacc catcgtgcgc ggtgacgaag ttgatgctgg
     8581 cgtgcggctt gcgcccgctc gcctcgtaga ggtccgagga gccggtgaag cggcgggcga
     8641 aatcgccgat cagcccgcca tcgcccttcc agtaggcgcg catcgtgtcg cggtacttgt
     8701 cgttccactc gttccagccc ggcgggaagt tgcccacctg gtagccgccg ctgccgatat
     8761 cccacggctc tgcgatcagc ttcacctgcg agagggcggg gtcctggccg atcgtgtcga
     8821 agaatgagcc gaggcggtcc acatcgaaca gctcgcgggc cagggcggaa gcgaggtcga
     8881 accggaagcc atcgacgtgc atctccagta cccagtagcg caagctgtcc atgagcagct
     8941 gcagcactcg tgggtgctgc agattgagcg tgttgcccgt gccggtgaaa tccatgtagt
     9001 agcgcggatt gtccggcacc agacggtaat aggcggcgtt gtcgatgcca cggaaggaga
     9061 gggtgggccc gagctgattc ccctccgccg tatggttgta gaccacatcg aggatcacct
     9121 cgatgcctgc cgagtgcaga gtcttgacca tcgtcttgaa ttcgctgatg cggccgctgg
     9181 cggcgtagcg cagctcgggc gcgaagaatc cgatcgtgtt gtagccccag taattgcgca
     9241 ggcctttttc caacaggtgc cgatcgtcca cgaacgcatg caccggcatc agctccagag
     9301 tcgtgatccc gagccgcaga agatgatcga cgacaggcgc agtcgcgatg ccggcatacg
     9361 tgccccgcag ctgcggcgga acctccgggt ggcgcatcgt caatccgcgc acgtgcgctt
     9421 cgtagatcac ggtgtcgtgc cagggcacgc gcggcggacg gtcgtccgcc cacgagaagg
     9481 ccggatcgac cacccggcat ttgggcgtac cggccgcgct gtcgcgccgg tcgaacgaca
     9541 gatcggcctt cgcgcccccc acccgatagc cgaagtgcga atcactccag ttcaaggggc
     9601 cctgcagatg cttggcgtac ggctcgatca ggagtttgtg agggttgaag cgctggcctt
     9661 tggcaggatc gtacgggccg tgcaccctgt agccgtagag ctgcccaggg cgagcctccg
     9721 gaagatagca atgccagatc tcatcggtgc gctcgcgcag tgcgatccgc tgcacctcgt
     9781 gccgtccggt agggtcgaac aggcacaatt ccactttctc cgcgttgccg gagaagatcg
     9841 agaaattgac cccctcgcca tcccatgtcg cgccgcgcgg gtagggttgc cccggccaca
     9901 ccgcgcgaat cgggcgcagc tcatgctgcg ctgaggctct ggccgtagtg gtctgctggt
     9961 ccatggaatg gaattggtct cagccggtga atgcgaggag gctgcgaaga ggaatgctcg
    10021 cccaatcggg ccggttcccg agctcatagc gcgcctcgta gagcgctttc tccagctcga
    10081 acaactcgag gagcggccgg acctccggga gtgagcggta caggccggct gcgctcgcga
    10141 tagcgtcata agtggtaaga aagacccgcc gcgtctcgct ctcccacttc tccagcatcg
    10201 gctcccacct gccaacatcg tcgggggtgt ggagcatgcc gcgcagcacc gctgcatgcc
    10261 gtgcatacga gaacgaacgc agcattccgg ccacatcgcg caacggggac tgtttcaggc
    10321 ggcgctcggc cagggagcgg cccggctcgc cctcgaaatc gacgatgatg aagtcgttgc
    10381 gcttcaccag aacctgaccc agatgataat cgccatgggg gcggatcttc aggccttgcg
    10441 gggccactga gccgagtgag tcgatgcgct tcaacagcgc cgcgcgccgc gccagtaccg
    10501 cttccacatc gggcagcaga gtctgtggga tcggcaaggg gggcttcgcg agctgctcga
    10561 gagttgcctc ggcttccctg cgggcattca ggcgccaggt cgcaatatcc tccgctcgga
    10621 ggggctcggg cgcgaacgca ggatcggaag tgggcgcagc gagggcgcga tgcagctcgg
    10681 cggtgcgcgt ggcgagggtc ttcatcaatt ccaggtacac gccatgcacg tcctcgcgca
    10741 gcgccacttc cgtgcgccgc tcttcgagga agcgtgagag gtagttgacg gtatagtccc
    10801 acccgtcacc ctggttggtc acgaaggcct gcagcaatgc gagcgtcacc gtgttgccat
    10861 ccgcattgcg gtactcggcc gcgccggcga ccggcacgat gctgggaaag cgtacgacct
    10921 cggtcagata gcggccgatt tcgagctcgg ggttgatgcc cgcctgcagc cggcgataga
    10981 tcttcagaaa gaactgatcg cccactctca cggtggtgtt gctgctctgc ccggctgtgg
    11041 gagtcacgct cagcggcgcg gactgatccc cggccagctg cgaatagact gccgtggcgg
    11101 acagatgcac ctcgccccgc tcggtgcgaa tcctggcgcc ctggccgatg gcctcgacca
    11161 ctgcccggca gaaattctca tccgtcgtgg catcggcgag gaccccgacc gtcgcctggc
    11221 gccgtacccg cgcgatggct ccgggctgca gcttctgcca gcggctctct tcggtatcct
    11281 cataagcgat ggcgaagggc aggaagtaca gcgctttgcc ggcgctgctc tccacttcga
    11341 acaccccggc aagccaccgg ccaagcggag tgtcccattc cccgttgtcg cccaggcgcg
    11401 cgcgcacgat cggtgcgccc ttgcctgcat accagcgctg cgaggcgatg tatttcggcg
    11461 ccacgcgggt ctcgagctgc tcgcgcagct tggtcgcaag tcccgagcgc cagttggcga
    11521 cacgctcggg aaagaagctc gtccagccat caaacagcac caacaccggc agatcctcgc
    11581 gaggcaggcg ttcgtcgtgc caggaggggg gctgctggtc gcggctgagc tggaaccagt
    11641 agaacccgta tccgggcagt gtcagcaggt aggggagatc gcccacgggt ggaaaaggtg
    11701 tgcgcccgat cagctcgatc ggcacggtgc ccttgaagtc cgccagatcc acctcgacgg
    11761 gctgagcgga gcgtgccagg ttgaccacgc acaggatcga atccggcccg tattcgcgca
    11821 ggtaaacgag cactttacga ttgccgggac ggatgaagcg cagcgtgccg cgaccgaaag
    11881 cctgcgaggt cttgcgcacc tgcagcatgc gcttcatcca gttgagcagc gaggagcggt
    11941 cgcgcgtctg cgcctcgacg ttcaccgcct cgtaaccata gatcggatcc atgatgggcg
    12001 gcagatagag ctgctgcgga tcggccctgg agaagcccgc attgcgatcg ggactccact
    12061 gcatgggagt gcgcaccccg ttgcgatcgc cgaggaagaa gttatctccc atgccgatct
    12121 catcaccgta gtagatgatg ggcgcccccg gcatggacag cagcaggctg ttcatcagct
    12181 tgatgcgctc ggggtcgttt tccatcagcg gcgccagacg gcggcggatg ccgagattca
    12241 aacgtgcgcg gggatcggct gcatacatgc ggtacatgta gtcgcgctcg cggctcgtca
    12301 ccatctccag tgtcagctcg tcgtggttgc gcagaaagat cgcccactga cacgtgtcgg
    12361 ggatgtccgg ggtctgctcc atgatctcga cgatcgggtg acggtcctcc tgggcgatgg
    12421 ccatgtacat gcggggcatc agggggaagt ggtaggccat atggcactcg tcaccgtccc
    12481 cgaagtagtc ccggacgtcc tcgggccact ggttcgcctc agcgagcagc atgcgatcgg
    12541 catagtgctc gttgatgact gcgcggatgc gcttgatgag ctcgtgcgtc tcgcgcaggt
    12601 tctcgttgct cgtgccctca cgctcgacga ggtagggaat cgcatccagc cgcagcccgt
    12661 ccacgccctg gtcgagccag tactccatga tgtgaatgac ggcccggaca acgtgcggat
    12721 tgtcgaaatt cagatcgggc tggtggctga agaaacgatg ccagtagtag gtcttggcca
    12781 gcggatcgag cgcccagttg gacgtctcgg tgtctcgaaa gatgacacgc gtgcctgcgt
    12841 aacgatccgg tgagtcgctc cagacgtagt agtcgcgctt gggcgagccg gccggcgcgc
    12901 ggcgcgcggc ctggaaccag gggtgtgcgt ccgaggtgtg attgatgacc agctcggtga
    12961 tcacgcgcag cccgcggcgg tgcgcctcct ggacaaacat ccggaagtcc ccgcgcgtgc
    13021 cgtaatgggc gtggatgttg tggtaatcgg agatgtcgta gccgtcgtcc ttgcccgggg
    13081 agggatagaa cggcagcagc cagatcgtat tgacgccgag atcctggatg tagtcgagct
    13141 tccgcgtcag gccgccaaag tcgcccacac catcatcgtt gctgtcgaag aacgcgcgga
    13201 cgtgcacctc atagatgacg gcgtccttgt accagagggg atccccggcc ggctgcggca
    13261 gttcgggctg tgcactcatc agcttgaaag cctacatgaa atagtcgaaa tctttttccg
    13321 acctcactcg tcggcggatg cggaacacgt gcgccggggc gcgcccggga tcgagtgaga
    13381 cgaagttccg cgcaccctgc cacagaaagc gggcgccgga gagcagatca tgcacctggt
    13441 aggagccgct cgccggcagg ccgaatgtgg gcagatcgag ctgcagccag ccgctctggg
    13501 ggttgtgcgg atcgaggttc accaccacca gcaccgagtc cgcgccgctc tcgcccgtct
    13561 tgctgtaggc gatcagcatg tcgctgtcgg tggcgtggaa cttcagtccg cgatcgctct
    13621 gcaggggcgg gttctcccgg cgaatgcgat tcacccgcgc g
//
LOCUS       DASRHN010000020         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_831365, whole genome shotgun sequence.
ACCESSION   DASRHN010000020 DASRHN010000000
VERSION     DASRHN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831365"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..>2526)
                     /locus_tag="VFB37_00645"
     CDS             complement(126..>2526)
                     /locus_tag="VFB37_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO20976.1"
                     /translation="VGQFLTTRTTRLAVDAVRSMQTEHEPRAQRASAVVEALVTYDRT
                     VIGYLQDGQASEVGQVTDAADALEAALRAYFQEPRSDRSLTAQLGTQLQPQLASHVEH
                     GRDLAKHATQRAEWLEQRHAALDRVQRRIVSAGGGGLAINGTQVVANRSLAELAAAIN
                     ALRGNFGSPAVLTGLERAFQAALAENSSELQRSPGRTWLDLEKEDFALAVRMRLAIDR
                     FDATNGAARREFLQESAALTAGVQKQLQEPARRGLVDAAAHAASAAEVAERTLTMTGV
                     AMLCVGLLISVLLMVSISVPVRRLTAATRLLASGDRRARAPRGGSAEIDALAESFNTM
                     ADQIARAEAELRAHQAELERHVADRTRQLHHLAHHDPLTQLPNRRQLSAHLGGALARA
                     MATGQRLALLFVDVDNFKSINDTLGHNFGDRVLQGIAERLGTVAHERALLARLGGDEF
                     TMLIEDVKSVEEVEARAAEIVTALQQPLTVDGRLLATSASVGASLFPDHADDTEQLLR
                     AADVALFRAKELGRNRYALYKPALYDAAAQRFRLEQSLRRAVEDGDLLLMFQPQIALH
                     SFEVTGLEALLRWRKPDGRIATATEFIHIAEKTGLIHELTDWVLRSATSTVAAWRAQG
                     WHRACVSINVSPPQFFESDFVDHIMRALEVTGLPASALELELTETVFQTGPTTIDSLR
                     RLRELGVSIALDDFGIGYSSLTSLEQLPITRVKLDRMLVEGVDSNPRSAAIVRSIIAL
                     CHGLGLQVIAEGVENEAECEALLRLGCTRAQGHYLGKPMAADDVVDLLREHATVTNDM
                     N"
BASE COUNT          416 a          817 c          887 g          406 t
ORIGIN      
        1 cgggccgccg aacccccgca atgacgagga ttaaagtagt gtcccagcca gtagcacggg
       61 gggagctact gcctgggact cgccaaccat aacgcagcgt agccgccgcg cacaataggt
      121 agctgtcagt tcatatcgtt cgtcacagtg gcgtgctcgc ggagcagatc cacgacatca
      181 tcggcggcca tcggcttgcc gaggtagtgg ccctgggcgc gggtgcagcc cagccgcagc
      241 agggcctcgc actcggcctc gttctcgacg ccctcggcga tcacctgcag cccgagcccg
      301 tggcacaacg cgatgatcga gcggacgatg gctgcggaac gcgggttcga atcgactccc
      361 tcgaccagca tgcgatcgag cttcacgcgg gtgatcggca gctgctcgag ggaggtgagc
      421 gaggagtatc cgatgccgaa gtcatccagc gcgatcgata cgcccagctc gcgcagccgg
      481 cgcagggagt cgatcgtcgt gggtcccgtc tgaaacacgg tctccgtcaa ctcgagctcc
      541 agagcacttg cgggcaggcc ggtcacttcc agcgcgcgca tgatgtgatc gacgaagtcg
      601 ctctcgaaga attgcggcgg ggagacgttg atcgagacac aggcgcgatg ccatccctgg
      661 gcgcgccacg cggccacggt ggaagtcgcc gagcgcagca cccagtcggt gagctcgtga
      721 atgagccccg tcttttcggc gatgtgaatg aattcggtcg cggtggcgat gcgcccatcc
      781 ggcttgcgcc agcgcagcag ggcctcgaga cccgtgactt cgaatgagtg cagcgcgatc
      841 tgcggctgaa acatcagcag cagatcgcca tcctccaccg cgcggcgcag cgactgctcg
      901 agccggaagc gttgcgcagc ggcatcgtac agggccggct tgtagagcgc gtagcgattg
      961 cgcccgagct ccttcgcccg gaagagcgcc acatcggcag cccgcagcag ctgctcggta
     1021 tcatcggcgt gatcgggaaa gaggctcgcg ccgacactgg cgctggtggc gagcaggcgg
     1081 ccatccacgg tgagcggctg ttgcagagcg gtgacgatct ccgcggcgcg tgcctcgact
     1141 tcctccaccg atttgacgtc ctcgatgagc atcgtgaact catcgccgcc gagccgggcc
     1201 aggagcgctc gctcgtgggc cacggtgccg agtcgctccg cgataccctg cagcacgcgg
     1261 tcaccgaagt tgtgtccgag cgtatcgttg atggacttga agttgtcgac gtccacgaag
     1321 agaagggcca ggcgctgccc cgtggccatg gcgcgcgcga gcgcgccgcc caggtgagcg
     1381 gaaagctgcc gtcggttggg caactgggtg agcggatcgt gatgcgcgag atggtgcagc
     1441 tggcgggtcc gatcggcgac atgacgctcg agctccgcct ggtgcgcgcg cagctcggcc
     1501 tcggcacgcg cgatctgatc ggccatcgtg ttgaaggact ctgccagcgc gtcgatctcg
     1561 gcggatccgc ctcgcggggc gcgtgcacgg cggtcgccgc tggcgagaag ccgcgtggcg
     1621 gccgtcagcc gccgcaccgg cacactgatg ctgaccatca acaacaccga gatcagcagt
     1681 ccgacgcaca acatcgctac accggtcatc gtgagggtgc gctcggcgac ctcggctgca
     1741 gaggcggcgt gggcggcggc atccaccagc ccgcgccgtg cgggctcctg gagctgcttc
     1801 tgcacaccgg cggtgagtgc agcgctctcc tgcagaaatt cgcggcgcgc ggccccgttg
     1861 gtcgcatcga agcggtcgat ggccaggcgc atccgcacgg cgagcgcaaa atcctccttt
     1921 tcgagatcga gccaggtgcg acccggcgag cgttgcagct cgctgctgtt ttccgccagt
     1981 gcggcctgga aagcgcgttc gaggccggtc agcacggcgg gcgagccgaa gttgccgcgc
     2041 agtgcgttga tggccgccgc gagctcggcg agcgagcgat tggcgacgac ctgtgtaccg
     2101 ttgatggcaa gtccgccgcc accggcggag acgatacgac gttgcacccg atcgagggcg
     2161 gcatggcgct gctcgagcca ctcggcgcgc tgcgttgcgt gtttggcgag atcccggccg
     2221 tgctcgacat gactcgcgag ctgcggttgg agctgcgtgc cgagctgtgc ggtgagcgag
     2281 cgatcgctgc gcggttcctg aaagtacgcg cgcagggcgg cttcgagcgc atcggctgca
     2341 tcggtgactt gcccgacctc ggaggcctgg ccgtcctgga ggtatccgat gaccgtgcgg
     2401 tcgtaagtga cgagggcttc gaccacggca ctggcgcgct gagcgcgcgg ctcgtgctcg
     2461 gtctgcatgc tcctcaccgc gtccaccgcg agacgcgtgg tgcgggtcgt cagaaactga
     2521 ccgacg
//
LOCUS       DASRHN010000021        19319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2149862, whole genome shotgun sequence.
ACCESSION   DASRHN010000021 DASRHN010000000
VERSION     DASRHN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19319
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149862"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFB37_00650"
     CDS             complement(<1..409)
                     /locus_tag="VFB37_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZO20977.1"
                     /translation="MGRQQQLRIAAYALLLLAASATQAHTRSHSRAVRVSADEPGLRS
                     ASALVLDATHASVLYSRKADVSAPIASITKLMTALVVADAGLPLDEPITLTAEDRIVG
                     KGAHSRLTLGATLTRGDLIHLALMSSENRAAHAL"
     gene            743..1174
                     /locus_tag="VFB37_00655"
     CDS             743..1174
                     /locus_tag="VFB37_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20978.1"
                     /translation="MTDMSVSFRVDFGPHCSVGPGKIALLENIGRTGSLSQAARALDM
                     SYRRAWLLLAETNDSFQQTVARMSVGGRGGGGATLTPFGTELVAAYRSLEANIRKQLP
                     TAFPASAVPSAAPQRLRTSSPIPRRRRAPPSPQRRARSRKS"
     gene            complement(1257..1526)
                     /locus_tag="VFB37_00660"
     CDS             complement(1257..1526)
                     /locus_tag="VFB37_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20979.1"
                     /translation="MRFKTHPGFAVATAAALLFGAAIASPASAADATVHCTGVNSCKG
                     QSACKGANNACKGQNSCKGQGFLEMSKKDCKAAQAKMKSEGDQKS"
     gene            complement(1613..2107)
                     /locus_tag="VFB37_00665"
     CDS             complement(1613..2107)
                     /locus_tag="VFB37_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZO20980.1"
                     /translation="MRLLFDWLIDWPARVARHLMWIAPLFARITVGWVFLLSGWGKLH
                     NLPRITQNFIDWGIPWPHFFTPLTSGIEFFGGMFLLLGLFTRISAGALGVTMIVAIRA
                     AKWGDVDSLETLLGFDEFEYLALFLWLAIAGAGVVSLDCLLTRRLGHRPALPSAGARL
                     ETSQ"
     gene            2387..2926
                     /locus_tag="VFB37_00670"
     CDS             2387..2926
                     /locus_tag="VFB37_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO20981.1"
                     /translation="MSQTRDAAQESLARLLESFRPDLVRFAFWLSRDPALAEDLAQET
                     MLRAWRARGELRSEAAARAWLFTIVRREFARLHERKRLQTVDVAECIARQDIELAFTD
                     PEPELVDLRRAILLLSDEYREPLLLQVLGGFSTEEIAQELGLTVPAVLTRLFRARNKL
                     KDALGATMPQGVMPAADAS"
     gene            2923..3654
                     /locus_tag="VFB37_00675"
     CDS             2923..3654
                     /locus_tag="VFB37_00675"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HZO20982.1"
                     /translation="MSCEGIRELIGADPDAVSPQVQAHLDDCAACRTWQAEQQTLNVR
                     IRRALQIDPQKLPAARQPRVISLVDRLHGRRRRAWTRGLPIAGSIAAGLLVAFTLWIT
                     RPGTSLASEVVSHVEGEPGSWSSTQRVSQEQLAAVLTRSQVAMGSGLGPVVYASSCRF
                     RGHVVPHLVVTTPSGPVTVMILEHERVRRQETFHEDGYSGLLVPAPAGSVAILSRTPM
                     ALEPQAAEVLHALDAANPGLPEAVR"
     gene            complement(3695..5044)
                     /locus_tag="VFB37_00680"
     CDS             complement(3695..5044)
                     /locus_tag="VFB37_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO20983.1"
                     /translation="MTSIAHSRWPALLALSACLVCSAAMAEEAPPSAAAVLAIHCGHL
                     IDTVDGKLLSETTVVVDGKRIREVTPGSTTPPGATPVDLSSQTCMPGLIDSHTHLTSQ
                     FGPTTYIDRFHWNLADYVLRSTVYARRTLLAGFTTVRNLGDSENESVALRNAINSGVL
                     MGPRIFTAGKAIGSTGGHADPTDGYRDDLAGDPGPTDGIINGVDAAMKAVREHYKKGD
                     DVIKIMPSGGVLDASSSGENPQLTLDEIKAVVSTAHDYGFTVAAHAHGAEAIRRAVVG
                     GVDSIEHGTYMNDEDMKLMKEHGTWYVPTIIAGDFVATKAKIPGYYPPQVAAKAAAIG
                     PLILGTAGRAYKAGVRIAFGTDAGVYPHGENAHEFELMVQAGMPPIFTIQAATTHAAQ
                     LLKHDKDLGSVTAGKLADLVAVPGNPLEDISLMKKVSFVMKEGVVYKREGQAVELSP"
     gene            5107..7809
                     /locus_tag="VFB37_00685"
     CDS             5107..7809
                     /locus_tag="VFB37_00685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZO20984.1"
                     /translation="MRTILGMSTGRNWRALTRAGLLVFSPILLLLAGAGATTTDVVRT
                     TLSNGLQIIVVRDPLAPVVATSVNYLAGSDEAPAGFPGLAHAQEHMMFRGSPGLTADQ
                     LADIGSVLGGNFNANTRESVTQYLFTVPAEDLDVALRVEATRMRGVLDSAADWKQERG
                     AIEQEVAQDVSNPSYILYEKVRADLFAGTPYAHDALGTRASFDKTTAGMLKAFYEAWY
                     APNNAILVVVGNVDPSATILEIRSLFGAIKRRKLPSRPAVSLQPARAASFTVDTDRPN
                     GTELLATRTPGLDSADFPALEVLSDVLSSRRFDLYGLVPQGKAIDAEFVLDPLPHAGL
                     AYTSVSFSSGDDPKALESTVRAILGHVASEGVPPELVEAAKIQERSAAQFPRNSISEL
                     ASIWSDAVALYGLESPDEDLARIEKVSVDDVNRVARKYLDLDHAVSAIMLPHGSGKPV
                     TAQGGGFGGREAISLGEAKPTPLPAWAATALRRVTLPPSTLNPVVSVLPNGLTLIVQT
                     ENVSDTISVFGHIRNRPETEEPAGQEGVHLVLDQLLTFGTESLDRVAFEQALDAIGAR
                     ARAGTDFSLQTLAEHFDQGIELLAQNELHPALPEPAMKIIQSQVAQGADARTRSPGWL
                     TQRSLRQALYPKTDPSLRVPTSQSVRSLTLQQVKDYYHLVFRPDLTTLVVIGKITPAQ
                     ARAAVEARFGGWSASGPKPNTDLPAAPPNRADVIAVPDDSRVQDVVVLAQNLPLTRAD
                     PDYYALELGNALLGGGFYSTRLSIDLRKNAGLVYSVGSILQAGRTRGAYLVEYASDPE
                     NVSRAAGLVVRDVKAMQTTPVSDDELTRIKAMLIRQIPLGESSVDEIAHGFLGRIDLG
                     LPLDEPTLAARRYVALNPQQIQAAFAKWLRPDDLVRITEGPAPH"
     gene            7920..8174
                     /locus_tag="VFB37_00690"
     CDS             7920..8174
                     /locus_tag="VFB37_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20985.1"
                     /translation="MASKIQGEGDYESARHYNDDTRRFVDEKRKEGKELKGSAKEASA
                     NLTPEEQAALARARGGDQDKRDADLLRNLEKHPEEKKPER"
     gene            8254..8850
                     /locus_tag="VFB37_00695"
     CDS             8254..8850
                     /locus_tag="VFB37_00695"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HZO20986.1"
                     /translation="MLLLRESRYPVVPWRNGGGLTREMLRDPPEPLPFDWRLSLATIE
                     RSGPFSHFPGYQRTLVLVGGAGIELSFGAHGGLRLCEVGKSVTFDGAWATDCTLLDGP
                     STDLNLMVSQARAAASTRCVRLTGEETVPTAGWLETFICCLSGSLEIIDSAGRMQSVE
                     TLDVARCFERDGPVTCKPGMSGVAHAFFASVRRLAHAL"
     gene            complement(8881..9258)
                     /locus_tag="VFB37_00700"
     CDS             complement(8881..9258)
                     /locus_tag="VFB37_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO20987.1"
                     /translation="MSEPNIIPKRVLVVDDNEDAAESMALVLKLEGHVAVSVYTAADA
                     LTHATSFRPDVVLLDIGLPEMDGYEVAQQMRELPGLKHVRIVAVTGYGRSEDRRRARE
                     AGFDAHLVKPVDIAVLQKVLAAG"
     gene            9558..10277
                     /gene="msrA"
                     /locus_tag="VFB37_00705"
     CDS             9558..10277
                     /gene="msrA"
                     /locus_tag="VFB37_00705"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZO20988.1"
                     /translation="MPLRPLRRSTCHTLPLLLGALGLTWQLLSCAAEPAVPVPPPAMD
                     NLRAAGPPQTAVLAGGCFWGVQGVYEHVRGVQRVTAGYAGGDPSTAHYDMVSTGTTGH
                     AESVQITFDPAQISYGEILQIFFSVVHDPTQLDRQGPDTGSQYRSDIDYTDGRQKSLA
                     LAYIAQLNAAHVFSAPIVTRVDPLRGFYPAESYHQDYLIHNPTAPYIAINDLPKVRNL
                     QRVFPQYYTGRPVLVSAASSR"
     gene            10285..10989
                     /locus_tag="VFB37_00710"
     CDS             10285..10989
                     /locus_tag="VFB37_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZO20989.1"
                     /translation="MSAPRPSHTAAALEEALSAAQRKAEELFAAIVEQGLIREGVLES
                     ELSEEIHALARARFGVRRHWHKRVVRCGPNTVLTYHAEPPDRRIEADDVVYLDFGPLF
                     EEWEADFGRTYVLGADPRKHRLVADIEVAFARGKQRFREDPHLTAGALYDYVRDLAQQ
                     AGWEFGAPTAGHLIGHFPHETAPADKRRLSICHGNELDLREPDSRGAPRHWILEIHFV
                     DTERTMGGFFEELLTI"
     gene            complement(11067..11219)
                     /locus_tag="VFB37_00715"
     CDS             complement(11067..11219)
                     /locus_tag="VFB37_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20990.1"
                     /translation="MGILKAIGVLLIVVWLVLWLFVKITFAAIHLLLLIGIVMLVVGF
                     MKRAST"
     gene            11446..12390
                     /gene="panE"
                     /locus_tag="VFB37_00720"
     CDS             11446..12390
                     /gene="panE"
                     /locus_tag="VFB37_00720"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZO20991.1"
                     /translation="MRILVVGAGALGGYFGGRLLQAKRDVTFLVRERRAAQLRSKGLR
                     IKSPTGDLELRDPPTVRAAQLRARFDLILLSCKAYDLQSAINDFAPAVDESSAILPLL
                     NGMHHLQVLDSRFGAHAVLGGQCLISSALDAEGQILHLNDLHSLSFGERDAPNSDRAT
                     TILEELTGAGFEARLSPRILQEMWEKWVFIAALGGITCLMRASIGDIAAAGAAPLAAR
                     LYGECARIAEDAGFPPGESMRQRSLAALTTPGSPLTASMLRDIESGGRTELDQILGDL
                     LMRRRSSATADEVDASLLSIACAHLRAYENRRRGAASG"
     gene            complement(12442..12876)
                     /locus_tag="VFB37_00725"
     CDS             complement(12442..12876)
                     /locus_tag="VFB37_00725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO20992.1"
                     /translation="MTQVRPLRSADRPQWADLWQGYLHFYRRHLPEQLTETTFARLID
                     PSRQPHGLVAEHEGRLIGLVHYLFHTSSWSMTDVCYLEDLYVDPAARGAGAGRALIEA
                     VYAAADRASVDTVYWLTQEFNADGRALYDTLAHRTSFIRYER"
     gene            complement(12901..13680)
                     /locus_tag="VFB37_00730"
     CDS             complement(12901..13680)
                     /locus_tag="VFB37_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO20993.1"
                     /translation="MLSEEECARCADEMLCAAVLRRPATRPSRTFPDITFQDACRIQD
                     LWAQMRLTRGGKLLGYKVGLTSAAARQALRAAEPSCGRIFEEDVHSPAEHIPADRFLK
                     PSLEVELAFIVHEALEGPELRSEDVLRIPHEVVPAFEIVDRRTELPRTLADTIADNAA
                     FGGLILGRQALHADVDLSRVGATLFRNGTIEDTGLAAAVMGHPAAAVAWLANKLHATG
                     ATLQKGQIVLTGAFLRPIDVRRGDRIAADYQPGGTLEALFV"
     gene            complement(14407..16362)
                     /locus_tag="VFB37_00735"
     CDS             complement(14407..16362)
                     /locus_tag="VFB37_00735"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZO20994.1"
                     /translation="MRPLDRVIAALAILVCLSACGGGGGGVAMPTLSSTAFTTNENVA
                     LPGQLSASGGSGMLSFSVLSKPASGTLTSFTSAGTFTYMPVANFVGSDSFTVQVSDSQ
                     GNMGTGTVAITVHANHAPTADNIVMRADGTALAKIDVLTSAHDPDGDPLTVSLADSAL
                     VGQATVNSDQSVSITGLPPGFKGLTRFRFTVTDPSGATASANAAIFVGADPFRALFAG
                     DASGDGTPEVYLTDFASDPQAVTTATQGDYRLAGFVGSPNGATVVYRSESAAGSTLTF
                     VQTAAVNKQVNIALPAGAALIQDAQGKDQFQVSPNGQWIAFVAGQSGASSVYLVNVAS
                     PTVLSRVAPAGAVFMSQLSFAADSNSLYFLATSVASGANESLYLVGLSDPTATALISA
                     ASAPGSTDTVVQYSISRDQTRILEEADRQGVTGLFYIDPQQLQDEILINPVLLSGQFI
                     PQSSFNSPPGIAVSPDGDQVAYSVQSLLGINMYASSVAPTPDPKLVATGARAIAFRPD
                     GAALLYTTGAQVDESPVASGATGQLVGNGGAAWYDSTGNIVLLEQFLASGGTPSSYPA
                     LASTVRGSFGTSQPLGTPLLAAQYFNVSGFDRAVVLLGEGPTTGPPAASVHLSLVNAL
                     APSKLFYLADYQTPLSLTSDVAEIVSE"
     gene            complement(16591..17118)
                     /locus_tag="VFB37_00740"
     CDS             complement(16591..17118)
                     /locus_tag="VFB37_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HZO20995.1"
                     /translation="MSERKRKELNRRRAAPLVTPSDLGTKASAEIAGGMNAILADVFA
                     LYLKTKNFHWHMSGPHFRDYHLLLDEQADQLYAMTDPIAERIRKVGGATLRSIGHIAR
                     LQRVKDNDAEYVDPLDMLAELREDNQSLAARLREVHDVVDEVHDIATASLIENWIDET
                     ERRTWFLFEASRQER"
     gene            17402..18106
                     /locus_tag="VFB37_00745"
     CDS             17402..18106
                     /locus_tag="VFB37_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZO20996.1"
                     /translation="MNTLADFAITNKWPARYPERIQLYSLPTPNGVKAAIMLEETGLA
                     YEPHRVNFDTQDQTSPEFLSLNPNNKIPALIDPNGPDGRPLPLFESGAILIYLADKTG
                     KFLAGAGGDRYQTLQWLMWQMGGVGPMFGQVGFFHKFAGRQYEDKRPLEHYVTESKRL
                     LRVLNERLVSRTWIMGDAYTIADIATFPWVRNLMGFYEAGDLVGISGYPHVLRALEAF
                     VARPAVASGLTIPREE"
     gene            complement(18169..18675)
                     /locus_tag="VFB37_00750"
     CDS             complement(18169..18675)
                     /locus_tag="VFB37_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZO20997.1"
                     /translation="MATAVFGMASYQAQITWRRGHHERFVDSRYSRAHEWHFDGGVVV
                     PASSAVHSVPLPYSRAENVDPEEALIAAISSCHMLTFLYLAAKARLIVDAYEDLAVGT
                     MSKDSRGRQAITSVVLAPKIVFSGPQAPSETEVARLHHEAHEQCYIANSVRSEIVVQG
                     EWRHQAGA"
     gene            complement(18746..19039)
                     /locus_tag="VFB37_00755"
     CDS             complement(18746..19039)
                     /locus_tag="VFB37_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO20998.1"
                     /translation="MLNAASRIVLGIVLIASMIATVVVVGTDRYSDSEAMLVKACAGG
                     RTDARLDYTLFNMRAQSIGGQNAICEPATPLDEDSAVTRQTARLDRHLIHQGR"
BASE COUNT         3434 a         6207 c         6180 g         3498 t
ORIGIN      
        1 cgagcgcatg ggccgcccga ttctcagagg acatcaaggc gaggtgaatc aggtcgccgc
       61 gcgtcagggt cgctcccagc gtgaggcggg aatgcgctcc cttgccgacg atccgatcct
      121 cggcagtcag cgtgatcggc tcatccaggg gcagcccggc atccgccacg accagtgccg
      181 tcatcagttt ggtgatggag gcaatgggcg ccgacacatc cgctttgcgc gagtacagca
      241 cggaggcgtg cgtggcatcg aggaccagtg cggaggctga acgcagtccc ggctcgtcag
      301 cgctcacccg tacggcgcgg gaatgagacc gcgtatgcgc ctgggtggca gaggcggcaa
      361 gcagaagcaa cgcataggct gcgattcgta gttgttgttg ccgtcccatc tgcgaaacct
      421 tgtcaaatca accctgatgc agtgtagcag agcacattgg aacgtggtgc accgtgaacc
      481 aactactggt tcagccgcaa ggcgcgtgcg gctttccgca cctccgctct tgcggctatg
      541 agcagccaac aattccacga ccacgcaggc gccgaaccca gtacggaccc gcaggagttc
      601 ctgcattatg gcaaaaaact ttatctgtag ctttgcaata aggacactgc gggcgcgggc
      661 gtaaaattgc ccgccctggg gtccctcaca gaattcgaga ggcctgcagc agtcgagccg
      721 tggcgtttgc ggaggacgga cgatgacaga catgtcggtg agctttcgag tggacttcgg
      781 tccgcactgc tcggtggggc cgggcaagat cgccctgctg gaaaacatcg ggcgcaccgg
      841 gtcgttgtcg caagctgccc gggccctgga tatgagctac cggcgtgcct ggctgctgct
      901 ggccgagacc aacgattcat tccagcagac ggtagcgcgc atgagcgtgg gaggacgggg
      961 cggaggcggt gcgacgctca cccctttcgg aacggaactg gtggccgctt atcgcagcct
     1021 cgaagcgaac attcgcaagc agctgccgac tgcatttccc gcaagtgcgg ttccttccgc
     1081 cgctccgcaa cgactgcgga cctcgagtcc tattccacgc agaaggcgcg cgccgccctc
     1141 cccacagcga cgtgcgcgct cgcgcaagag ttagatgccg gagcggcggt cagacatcac
     1201 acctgatcgc cgctccggcg cgcgcatcct tgcccgcgtg cttcggtcgc tagtcctcag
     1261 gatttctgat cgccctcact cttcattttc gcctgggcgg ccttgcagtc cttcttgctc
     1321 atctcgagga agccctgccc cttgcaggag ttctgtccct tgcaggcgtt gttggcgccc
     1381 ttgcatgcgg attgcccttt gcaggaattg acgcccgtac agtggaccgt tgcatcggcg
     1441 gcggaggctg gggaggcgat cgccgcacca aagagcagcg ccgcagcggt tgcgacggcg
     1501 aagccaggat gggtcttgaa gcgcatggtc atgtcctttc ggattgtgga aggatgaggg
     1561 atcgccgctg tgcgcggctc accccggtga gcagaccgct tcaaggactg ctttattgcg
     1621 aggtttcgag ccgcgcgccg gcgctgggca aagctggccg gtggccgagg cgccgcgtga
     1681 gcaggcaatc gagcgacacc acgcccgcac ctgcgatcgc aagccacaaa aacagcgcca
     1741 ggtactcgaa ctcgtcgaat ccgagcaggg tctcgagaga atcgacgtcg ccccacttcg
     1801 ccgcgcggat ggccacgatc atcgtgacgc cgagggcccc ggccgagata cgcgtgaaca
     1861 gtcccagcag caggaacatc ccgccgaaaa actcgatgcc cgaggtcagc ggcgtgaaga
     1921 agtgcggcca gggaatgccc cagtcaatga aattctgggt gatgcgcggc aggttgtgga
     1981 gcttgcccca gccgctcagg aggaagaccc agccgacggt gatgcgggcg aacagcggcg
     2041 cgatccacat caggtgcctg gcgacgcgcg ccggccagtc gatcagccaa tcgaagagca
     2101 atcgcatcgc cgtctcccct ttctcgagcc gcaaccgcgg cctgttgagc gatattagac
     2161 cgtcgcggcc gagtcgttat tgcaagtgcg gcctcctggc gcagcgcaat ggatcgccca
     2221 cgagcccggt ctgatgaagg tggccctcat ccatcgcacc ccggctgtgg ccgggctcca
     2281 ccgtgcgcca gcggtgtttg ggccccgatg gggcgattct gcgggctatc cgctacagtg
     2341 tgtccccctg cacagagtgc atcggccgcc gatgatcgaa cggcagatga gccaaacccg
     2401 ggacgccgct caggaatcgc tggcgcgcct gctcgaatcc ttcaggcctg acctggtgcg
     2461 cttcgctttc tggctgtcgc gcgaccccgc gctggccgag gatctggctc aggagaccat
     2521 gttgcgagct tggcgcgcgc ggggggagct gcgcagcgag gccgctgctc gagcctggct
     2581 gttcacgatc gtgcggcgcg agtttgcgcg gttgcacgag cgcaagcgcc ttcagaccgt
     2641 tgacgtggct gagtgcatcg cgcgtcagga tatcgagctt gccttcaccg accccgaacc
     2701 ggagctggtg gatttgcgac gcgccattct cttgctgtcc gatgaatatc gagagcctct
     2761 gctgttgcag gtgctggggg gcttctcgac cgaagagatc gcccaggagc tcggcctcac
     2821 ggtgccggca gtcctgacgc ggcttttcag ggcgcgcaac aagctgaagg atgcgttggg
     2881 ggccaccatg ccccagggcg tgatgcccgc ggcggatgca tcatgagctg cgaggggatc
     2941 cgtgagctga tcggtgccga cccggatgcc gtctcaccac aggtgcaggc gcatttggat
     3001 gattgtgcgg cgtgccgcac gtggcaggcg gagcagcaaa ctttgaacgt gcgcatccgt
     3061 cgggcgctgc agatcgaccc gcagaagctg ccagccgcca ggcagcctcg tgtcatctcg
     3121 ctggtcgatc ggctgcacgg caggcggcgg cgtgcctgga ctcgcggcct gcccattgcg
     3181 ggcagcatcg ctgcagggct gctcgtcgca ttcacgttgt ggatcactcg acccggcacg
     3241 agccttgcgt ccgaggtggt ctcacacgtt gagggcgagc caggcagctg gagcagcacg
     3301 caacgggttt cccaagagca gcttgcggcc gtgctgaccc ggagccaggt ggcgatgggc
     3361 tccggactgg gccccgtggt ttatgcctcg agctgccgct ttcgtggtca cgtggtaccc
     3421 cacttggtgg tcacgacgcc ttcgggaccc gtcaccgtga tgatcctgga gcatgaacgc
     3481 gttcgcaggc aggagacatt ccacgaggac ggctattcgg gtctgttggt tcccgcacct
     3541 gcgggcagcg ttgccatact cagccgtacg cccatggcac tcgagccgca ggccgccgag
     3601 gtgctgcatg ccctcgatgc agccaacccc ggattgcccg aggcagtgcg ctgactcgaa
     3661 cccccgaccg cgcagctcca ggcctcacgc catcttatgg actgagctcg accgcctgac
     3721 cttcgcgctt gtacaccacg ccttccttca tgacgaagct gaccttcttc atgaggctga
     3781 tgtcctcgag cggattgccg ggcacggcga cgaggtccgc gagcttgccg gcggtcacgc
     3841 tgcccaggtc cttgtcatgt ttgagcaatt gggccgcgtg cgtggtcgcc gcctggatcg
     3901 tgaagatcgg tggcatccct gcctggacca tcagctcgaa ctcgtgggca ttctcgccgt
     3961 gaggatagac cccggcatcg gtcccgaagg caatcctcac tccggctttg tacgctcgtc
     4021 cggcagttcc aaggatcagc ggtccgatcg cggccgcctt ggcagccact tgcggagggt
     4081 agtaccccgg gattttcgcc ttggtggcca cgaagtcacc cgcgatgatc gtcggcacat
     4141 accaggtgcc gtgctccttc atgagcttca tgtcctcgtc attcatgtag gtaccatgct
     4201 cgatggagtc cactccgccc accaccgcgc ggcggatcgc ttcggctccg tgcgcatgag
     4261 ccgctaccgt gaatccgtag tcgtgtgcag tgctcaccac cgccttgatc tcatccaggg
     4321 tcaattgtgg attctcgccg ctcgagcttg catccaacac gccaccggac ggcatgatct
     4381 tgatgacgtc gtcccccttc ttgtagtgct cccgcacagc cttcatggcg gcgtcgacac
     4441 cattgatgat gccgtccgtc gggcccggat cgccggccag atcgtcgcga tacccatccg
     4501 tcggatccgc atgcccgccg gtcgatccga tggccttgcc ggccgtgaag atgcgcggcc
     4561 ccatgaggac ccccgagttg atggcgttgc gcagggcgac cgactcattc tcggagtcgc
     4621 ccagattgcg gacggtcgtg aaccctgcga gcagcgtgcg ccgcgcatag accgtggagc
     4681 ggagcacgta gtcggcgagg ttccaatgga acctgtcgat atacgtggtc ggaccaaact
     4741 gcgaggtcag atgagtgtga ctgtcgatca ggcccggcat gcaggtctgc gaagacagat
     4801 cgaccggggt tgcgcccggc ggagtcgtcg atcccggggt cacttcgcgt atccgcttgc
     4861 cgtccacgac cacggtcgtc tcgctcagca atttgccgtc gacggtgtcg atgagatggc
     4921 cacagtggat agccagcaca gctgccgcgc tcggcggagc ttcctccgcc atcgcggccg
     4981 agcagacaag gcatgcggac agggccagca gagctggcca gcgcgagtgg gcgattgatg
     5041 tcacggtgtg ctcccttcgt gcaatccaga tgatgagtcg cagtcaaatc gcataaggta
     5101 tacgggatgc gcacaatact gggaatgagt accggccgaa actggcgcgc gctgacacgg
     5161 gccggccttc tggtgttcag cccgatcctt ctgctgcttg cgggggccgg ggcaaccaca
     5221 accgatgtgg tccgtaccac cctctcgaac ggactgcaga tcatcgtcgt gcgcgatccg
     5281 ctggcccctg tcgtcgcgac gtcggtgaac tatctcgccg gctcggatga ggcgcccgcg
     5341 ggctttccgg gcctggcaca cgctcaggag cacatgatgt tccgcggcag tccggggctc
     5401 accgctgatc agctggccga catcggcagc gtgctggggg gcaacttcaa cgcgaacaca
     5461 cgcgagagcg tgactcagta cctgttcacg gtcccggcgg aggatctgga cgtcgcgctg
     5521 cgggtcgagg ccaccaggat gcgcggggtg ctggacagcg ccgccgactg gaagcaggag
     5581 cgcggggcca tcgagcagga ggtagcccag gacgtgtcca acccgagcta catactctac
     5641 gaaaaggtcc gtgcggacct gttcgccggc accccgtacg cgcatgacgc cctgggcacg
     5701 cgagcctcgt tcgacaagac gactgctggg atgttgaagg cgttttacga ggcgtggtat
     5761 gcgccgaata acgcgattct cgtggtggtc ggtaacgtcg acccttccgc gaccatcctc
     5821 gagatcagga gcctgtttgg cgccatcaag cgcaggaagc tgcccagcag gcctgcggtg
     5881 agtctccaac cggcccgcgc ggcttctttc acggtggata cggatcgtcc caacggcacg
     5941 gagcttctgg ccacgcgcac gccagggctg gacagcgccg acttccccgc gctggaagtt
     6001 ctctccgacg tgctcagcag tcgccgtttc gatctgtacg ggctcgttcc tcagggcaag
     6061 gcgatcgatg ccgagttcgt cctcgatcca ttgccccacg cagggcttgc ctacaccagc
     6121 gtctcctttt cctcaggcga tgatccgaag gcgctcgaga gcacggtgcg cgccatactc
     6181 ggccacgtcg cgagcgaagg cgtgcccccg gagctggtgg aagcggcgaa aatccaggag
     6241 cgcagcgccg ctcagttccc ccggaactcc atctcggagc tggcttccat ctggtcggat
     6301 gcggttgcgc tctatgggct cgagtccccc gatgaggatc tggcgcggat cgagaaggtc
     6361 agcgtggatg acgtgaatcg ggtcgcccgc aagtacctag atctcgatca tgccgtctcc
     6421 gccatcatgc tgccgcacgg ctcaggcaaa ccggtgaccg ctcaaggcgg cggcttcgga
     6481 ggccgagagg caatctctct cggagaggcg aagccgactc cactgcctgc gtgggccgcc
     6541 accgcgctgc gccgcgtgac cttgccgcct tcgaccctca atccggtcgt cagcgttctg
     6601 cccaacggac tcacactcat cgtgcagacc gagaatgtga gcgacacgat cagcgtgttc
     6661 ggacatatcc gcaaccgtcc agagaccgag gagcctgccg gacaggaggg cgttcacctg
     6721 gtgctggatc agctgctgac cttcggtacc gaatcgctcg atcgggtggc gttcgagcag
     6781 gctctggatg cgatcggcgc acgcgcgcga gccggcacgg atttctcatt gcagacactc
     6841 gcggagcact tcgatcaggg tatcgagctg ctggcgcaga acgagctgca tccggccctg
     6901 cctgagccgg ccatgaagat catccagagt caggtcgcgc agggagcaga cgctcgcacg
     6961 cgcagccccg gctggctgac gcagcgttcg ctgcgccagg ccctttatcc gaagactgac
     7021 ccgagcctgc gcgtgccgac ttcccagagc gtgcgcagcc tgacgctcca acaggtcaag
     7081 gactactatc acctcgtttt tcgacccgac ctgacgactc tcgtcgtcat tggcaagatc
     7141 actccggcgc aggcgcgggc cgccgttgag gcccgtttcg gcggctggag cgcctcaggt
     7201 ccgaagccca acactgacct gcccgcggcg ccccccaacc gagcggatgt catcgccgtg
     7261 ccggatgaca gccgggtgca ggatgtcgtc gtcctcgcgc agaatctgcc gctcacccgc
     7321 gccgatccgg actactacgc gctcgagctg ggcaacgcac tgctcggcgg tggcttctat
     7381 tcgacgcgcc tcagcattga tctgcgcaaa aacgcgggcc tcgtgtactc ggtaggctcc
     7441 atcctccagg ccggcaggac gcgcggcgct taccttgtcg aatacgcgag cgatcccgag
     7501 aacgtcagtc gtgccgcggg cctggtggtt cgagacgtca aggccatgca aacgactccc
     7561 gtcagtgatg acgagctcac ccggatcaaa gcgatgctca tccgacagat cccgctggga
     7621 gagtcgagtg tcgatgagat cgcgcacggc ttcctggggc ggatcgatct cgggttgccc
     7681 ttggacgagc cgaccctcgc ggcccgccgc tacgtcgcac tgaatccgca gcagatccag
     7741 gctgcgttcg cgaagtggct gcgccccgat gacctcgttc ggatcaccga agggccggcg
     7801 ccgcactgaa gcccgggctg tgccgtgaga gcgccggcag cagctcggag ccagcgaggg
     7861 catggtattt gctctgtatc gccatcactt ccaatgacga cgcactacgg agcatacgca
     7921 tggcatccaa gattcagggc gaaggcgatt acgaatctgc acgccactat aacgatgaca
     7981 cccggcgctt cgtcgacgag aagcgcaagg agggcaagga gctgaagggt tcggccaagg
     8041 aagcctcggc gaacctcacg cccgaggagc aggctgccct tgcacgcgcg cgcggcggcg
     8101 atcaggacaa gcgggacgcc gatctgctgc gaaatctgga gaaacatccc gaagagaaga
     8161 agcccgagcg ctgagcgccg gccgagagca ggttctggct gctgcccgcg gtggcgatgc
     8221 cataatcgcg catcgcactc aggcccctat tgaatgctgc tgctgcggga atcgcgatat
     8281 ccggtcgtgc cgtggagaaa tggcggcggg ctcacccggg agatgctgcg ggacccgcct
     8341 gagccgctcc ccttcgactg gcggctcagc ctcgcgacca tcgagcgctc gggccccttc
     8401 tcgcactttc ccggctatca gcgcaccctg gtgctggtcg gcggcgccgg aatcgagctg
     8461 agcttcggtg cgcacggtgg cttgcgactt tgcgaagtcg gaaagtcggt gacattcgac
     8521 ggggcctggg ccacggactg caccctgctc gatgggccca gcaccgacct caatctgatg
     8581 gtgtctcagg cgcgggccgc ggcctcaact cggtgtgtgc ggctgacagg tgaagaaacc
     8641 gtcccgaccg ccggttggct cgagacgttc atctgctgtt tgagcggatc gctcgagatc
     8701 atcgactcgg ccggaaggat gcagagcgtt gaaacgctcg acgtggctcg ttgcttcgaa
     8761 cgcgacggac ctgtcacctg caagcctggc atgtcgggcg tggcccatgc atttttcgcc
     8821 tcggtcagac gactcgcgca cgcactgtag gctcacgatg tgcctgctct tgcacgcata
     8881 tcaacctgcg gcgagaacct tctgaagaac cgcaatatcg accggtttca ccagatgagc
     8941 gtcaaagccg gcttcccgcg cgcgccgcct gtcttccgat cgcccgtaac cggtcacggc
     9001 cacgattcgc acgtgcttca gcccgggcag ctcgcgcatc tgctgtgcaa cctcgtagcc
     9061 gtccatttcg ggcagtccga tatcgagcag caccacatca ggccgaaacg atgtggcatg
     9121 agtcagcgca tcggcggccg tatagaccga cacggccaca tgtccctcga gtttcagaac
     9181 gagggccatc gattcggcgg catcctcatt gtcatcgacc acgagcacgc gcttcgggat
     9241 gatattcggc tcactcatgc gcgcatgtta atgggtggct cgcccttcga gccggccaac
     9301 cgggcagggc cgacgtctgt gatcggaaga aaccttaaga tagacttgct catccggacg
     9361 gattacgcaa acgacccgac gacggcctcg cgagtcggct cgggcgccat gtgcaggaaa
     9421 ccgtggtgcg ccgaatcgct gcgaaatgat gaccgcccga agccggtctg cttggcatac
     9481 gcagccgcag ccgccatctg ggcgatgtcg gctcccgact gagcacaccg gcgccaaacg
     9541 cgcctctgga gcttcccgtg ccccttagac ccctgcgcag atcaacctgt cacacacttc
     9601 ctctgctgct gggcgcgctt ggcctgacat ggcagctgct ttcctgcgcc gcagagccgg
     9661 cggtacccgt tccgcccccg gcgatggaca atcttcgggc ggccggaccg cctcagaccg
     9721 cagtactggc gggcggctgc ttctggggcg tgcagggcgt ctacgagcac gtgcgcggcg
     9781 tgcagcgggt cacggcgggc tacgccggtg gagacccttc tacggcgcac tacgacatgg
     9841 tgagcaccgg gacgacaggt cacgccgagt ccgtacagat caccttcgac ccggcgcaga
     9901 tctcgtatgg cgagatcctg cagatctttt tctccgtcgt tcatgatcca acacagctcg
     9961 accggcaggg cccggacacc ggcagccagt accgctcgga catcgattac acggacggga
    10021 ggcagaagag cctcgcgctc gcgtacattg cccaactcaa cgcggcgcat gtcttctccg
    10081 ccccgatcgt cacccgcgtt gatcccctgc gaggtttcta cccggcggag agttatcatc
    10141 aggactactt gattcacaac ccgacggcgc cctacatcgc gatcaatgat ttgccgaaag
    10201 tgcgcaatct gcagcgcgtc tttccccagt actacaccgg tcggccggtg ctggtgagcg
    10261 ccgcctcatc ccggtaaaag ctacatgtcc gcgccacgac cgtcacacac cgcagccgct
    10321 ctcgaagagg ctctgagcgc tgcgcagcgc aaggccgaag agctgtttgc ggccatcgtg
    10381 gagcaagggc tgatccggga gggtgtgctc gaaagcgagc tgagcgagga gatccacgcc
    10441 ctggcgagag cgcgcttcgg cgtacgccga cattggcaca agcgggtcgt gcgatgcgga
    10501 cccaataccg tgctcaccta tcatgccgag ccgccggacc gccggatcga ggcggatgac
    10561 gtggtgtacc tcgacttcgg tccgctgttc gaggaatggg aagcggattt tggacgcact
    10621 tacgtgctcg gcgcggatcc gcgcaagcac cgtctggttg ccgacatcga ggtcgccttc
    10681 gcgcggggca agcagcgctt ccgggaagat cctcatctca ccgctggcgc gctctacgac
    10741 tacgtgcgcg atctggcgca gcaggcgggc tgggaatttg gcgcgcccac cgccggccat
    10801 ctcatcggcc atttcccgca cgagaccgct ccggcagaca aacggcgcct ctcgatctgc
    10861 catggcaacg agctcgacct gcgcgagccc gactcgcgcg gcgcaccgcg ccactggatc
    10921 ctggagatcc acttcgtcga tacagagaga acgatgggcg gattcttcga ggagctgctg
    10981 acaatctgat ggcccggcca agcggccgtc agccgcgagc gcctgacctc gcgcacgcgg
    11041 gcggcctcaa tgggctgcca ggcacctcac gtactcgcgc gcttcatgaa ccccacgacc
    11101 agcatcacga tgccgatcaa cagcaacaga tgaatcgctg caaacgtgat cttcacgaac
    11161 agccacagca ccagccacac tacgatgagc agtacgccga tcgccttgag aatacccatc
    11221 tgtcctccgc aacggtttgc gtcctggcct gaagtttacg cgtgccttcg gtcggccagc
    11281 gcacgtgccg cgaggttccg gcttccgcca cccgatccgg tgttgggttc ctacgagctt
    11341 ttcagtctgc ggcctacaga ccgagcctaa cggcggcatt cccgtccggc ggcgctcagg
    11401 ccgcgcaagc cgagtgggtt acgatagggt aaggaggagt tccgaatgcg aattctcgtg
    11461 gtcggcgccg gcgcccttgg tggctacttt ggaggccggt tgctgcaggc caagcgtgac
    11521 gtgaccttcc tggtacgtga gcggcgcgca gcgcaattgc gcagcaaagg cctgcgcatc
    11581 aaaagcccca ctggcgatct ggagctgcgc gatccgccga cggtgcgggc tgctcagctg
    11641 cgcgcgcgat tcgatctgat tctgctgagc tgcaaggcct atgacctgca gagtgcgatc
    11701 aatgacttcg ccccggcggt cgatgagagc agcgcgatcc tgccgttgct caatggaatg
    11761 caccatctac aggtgctgga cagccgcttt ggtgcccatg cggtgctcgg cgggcagtgc
    11821 ctgatctcct ctgcgctgga tgcggagggc cagattctgc atctcaacga tctgcacagc
    11881 ctcagtttcg gtgaacgcga cgcgccgaac tcggaccgcg ccaccaccat tctcgaggag
    11941 ctgacgggag ccggcttcga ggcgcggttg agcccgagga tcctccagga gatgtgggag
    12001 aaatgggtgt tcatcgcggc gctgggcgga atcacttgtc tcatgagggc aagcatcgga
    12061 gacatcgccg cggcgggcgc cgcgccactc gctgcgaggc tctatggaga atgtgcccgg
    12121 attgcagagg atgcaggctt tcctcccgga gaaagcatgc gtcagcggag cctcgctgct
    12181 ctgacgacgc cgggctcacc gctcaccgct tcgatgcttc gcgatatcga gagtggcggc
    12241 cgaacggaac tggatcagat cctgggagac cttctcatgc gccggcgcag ctccgccacc
    12301 gcagatgagg tggatgcctc gctgctgagc atcgcctgcg cacacctgag ggcatatgag
    12361 aaccgtcgcc ggggtgcggc ttcgggctga gttcgccgct cgcgatcgtc ttggcggccc
    12421 gaaactttcg cgccgggacg ctcaccgttc atatcggata aaggatgtgc gatgcgccag
    12481 ggtgtcatac agagcacggc cgtcggcgtt gaattcctgc gtcagccagt agactgtgtc
    12541 aacactcgcg cgatccgccg cggcataaac agcttcgatc agcgcccgtc ccgcgcctgc
    12601 gccgcgcgct gccggatcaa catatagatc ctccagatag cacacatcag tcatcgacca
    12661 ggaggaggta tggaacagat agtgcacgag cccgatcagt ctgccttcat gctcagccac
    12721 caggccgtgc ggctgacgag atggatcgat gaggcgtgca aaggtcgtct ccgtcagctg
    12781 ttcgggcaaa tggcgcctgt aaaagtggag gtacccttgc cacagatctg cccattgagg
    12841 gcgatccgcg gatctcagtg gacggacttg ggtcatggtg gcttcccgtg tgcggccgat
    12901 tcagacaaac agcgcctcga gcgtaccacc gggttggtag tccgcagcga tccgatcacc
    12961 ccgccgcaca tcgatcgggc gcaggaaggc gccggtaagc acgatctgcc ccttctgcag
    13021 cgtagcgccc gtagcgtgca gcttgttggc gagccaggcg acagccgcgg cggggtgtcc
    13081 catcaccgcg gccgccaggc ccgtatcttc gatggtgccg ttccgaaaca gggtcgcgcc
    13141 cacccggctc agatcgacat cggcgtgcag ggcttgccgg ccaagaatga ggccaccgaa
    13201 agcggcgtta tcggcaatgg tgtccgcaag ggttcgcggc agttcggttc gtcgatcgac
    13261 gatctcgaag gcgggcacga cttcatgggg gatcctcagc acgtcctcgc tcctcagctc
    13321 gggtccttcc agagcctcgt gcacgatgaa tgcgagctcc acttcgaggc tcggcttgag
    13381 gaaccggtca gccgggatat gctctgccgg actgtgaacg tcctcttcga aaatgcgccc
    13441 gcaactcggc tccgcggccc gcagtgcctg gcgcgcggct gctgaagtca ggccaacttt
    13501 gtagcccaga agcttgccgc cacgagtcag ccgcatctgc gcccacagat cctgaatccg
    13561 gcaggcatcc tggaaggtga tatcgggaaa tgtgcggctg ggtcgggtgg cgggcctcct
    13621 cagcaccgcc gcgcacagca tttcgtcggc gcagcgcgcg cactcctcct ccgagagcac
    13681 gtcaacctcc ttccggcctg gctcaccgcg ttcgtcgcgg agcctacacc tatgacgagg
    13741 ttgcctgcac ggtcgcgctc acgtggagga atggtgcgcc gagcggctgc gcgcgccttt
    13801 gcgggctgcc atcgatcgcc cgctccgtcc tttggtcgcg gatgccttac gagccgaagc
    13861 cgaccgctcg tccggagttc gccggcgcgc cgccgacttc gcttgggcgg acaacgctcg
    13921 ccgcgaagcg gcggccgggc tttcgcggcg aagcgcctga gtcgaggccc tgcgccgctt
    13981 ggcagtggtc ttgcgccgag gcggcgcgcc atgaccgagc gcgtaagcgc gcgctgcgct
    14041 tcgccgtgtc ttgctcgagg cctgaccccg cgcgggcggc ggcagatcta cgcccgcacg
    14101 cctggccttg gacaacccga tggcgatcgc ctgcttgctc gagcgtgctc cgtgctggcc
    14161 tgcgcggata tgctccatct cctcgcggat gaactcgcca gcctgagtgg ttggcgcctt
    14221 cccctgttgc ttgtcccgac gtgctcgctg caaagtcttc ctgtctggca tgttcagctc
    14281 ctgctcgctg ggtgaaccgc acccgagcat caagctaggc gcaggcaacg caggcgcgcc
    14341 atcagccggc aactcactcg ttcgtgaggg atctcctacg agtctctgga cgcgtaccgc
    14401 tttgcattat tcgctgacga tttctgcgac gtcggaggtc agtgagagag gagtctgata
    14461 gtccgccagg tagaacagct tgctcggcgc cagagcattg acgagtgaga ggtggacact
    14521 cgcagcgggc ggtcctgtcg tgggtccttc gcccaacagg acgaccgccc gatcgaagcc
    14581 cgagacattg aaatactgcg cggcgagcag cggggtcccg agcggctgag aagtgccaaa
    14641 ggagccgcga accgtcgagg ccagcgccgg gtaggaggag ggggtgcctc ccgaggcgag
    14701 gaattgctcg agcagaacga tgttgcccgt cgagtcatac caggctgccc cgccattgcc
    14761 cacgagttga cctgtggccc cgctcgcgac ggggctctcg tcaacctgcg cgcccgtcgt
    14821 gtacaggagc gccgccccat cgggacggaa ggcgatcgcc cgagcgcccg tagccaccag
    14881 cttcggatcc ggtgtcggcg ccacactcga ggcatacatg ttgattccca gcagggactg
    14941 cacggaataa gcaacctgat cgccgtctgg gctcacggca atgcccggtg gggaattgaa
    15001 agacgactgc ggaatgaact gccctgacaa cagcaccggg ttgatcagga tttcatcctg
    15061 cagctgctgc ggatcgatgt agaacaggcc cgtaacgcct tgccgatcgg cctcctcgag
    15121 aatccgggtc tggtctctcg agatggagta ctgcacgacg gtatcggtgc tacccggtgc
    15181 gctcgcggcg gagatcaggg cggtcgccgt cgggtctgaa agcccgacga ggtacaacga
    15241 ctcgttcgct cccgaagcca cactggttgc aaggaagtac aggctgttcg aatcggcggc
    15301 gaagctcaac tggctcatga ataccgctcc ggctggagcc acacggctga gcaccgtcgg
    15361 actggccacg ttcacgaggt acaccgaact ggcgccgctc tgcccggcga cgaaggcaat
    15421 ccattgcccg ttcggactga cctggaactg atcctttccc tgcgcgtcct ggatgagcgc
    15481 cgcgccggcg ggcaaggcaa tgttcacctg cttgttcaca gcggcggtct gcacgaacgt
    15541 gagcgtcgag ccggccgcgg actcactgcg gtagacgacc gtggccccat tgggcgagcc
    15601 cacaaatccc gcgagccgat agtctccctg tgtcgcggtg gtgacggcct gagggtccga
    15661 ggcaaagtcg gtcaggtaca cctccggtgt gccgtcgccg gaagcatctc cggcgaacag
    15721 ggctcggaac gggtccgcgc cgacgaagat ggccgcattc gcactggcgg tggcaccgct
    15781 ggggtccgtg acggtgaagc ggaatcgggt cagtcctttg aatccgggtg gcaggccagt
    15841 gatgctgacg ctctggtctg aattgacggt ggcttggccc acgagggccg aatcggcaag
    15901 ggacaccgtc aatggatcgc cgtcggggtc gtgcgcgctc gtcagcacgt cgattttggc
    15961 gagcgctgtt ccgtccgccc gcatgacgat gttgtcagcg gtgggcgcat gattcgcgtg
    16021 cacggtgatg gcgacggttc ccgtgcccat gtttccctgg gagtcgctaa cttgtaccgt
    16081 gaaagagtcg gagccgacga aattggcgac cggcatgtag gtgaacgtgc ctgcagaggt
    16141 gaagctggtg agcgtgccgg aagcgggctt gctcagcacg gagaagctca gcatgccgct
    16201 gccaccgctt gcgctgagtt gtccgggcaa tgccacgttc tcattggtcg tgaacgcggt
    16261 ggagctgagt gtcggcatgg ccacgccacc gcctccaccg ccgcacgcgc tgaggcagac
    16321 caggatcgca agcgccgcga tcacgcgatc taacgggcgc atcttcactc gacactgcaa
    16381 ctgcacgatc gacccccgcg aggtttcgaa ggttggatag tgccacagct ttgccggcct
    16441 caatcacgcg ttgcgcaaca acaacgaaag gcttgtcaag ctcacgccag gcctgacggc
    16501 aacgttctcg cccgaggtgt tggtttgtgc tgtgttggat tacggcttta cagtgaagtc
    16561 aacgtgtcgg cggctcgcga cagccgcgcc tcagcgctcc tgtcggctgg cctcgaacag
    16621 aaaccaggtg cgccgctcgg tctcatcgat ccagttctcg atgagactcg ccgtggcgat
    16681 gtcatgcacc tcatcgacca cgtcatgtac ctcgcgcagg cgtgccgcga gggattgatt
    16741 gtcttcgcga agctcggcga gcatgtccag cgggtcgaca tactcggcgt cgttgtcttt
    16801 gactcgctgc agccgtgcga tgtgcccgat cgagcgcagc gtcgcgccac cgaccttgcg
    16861 gatgcgctcg gcaatcggat ccgtcatggc gtagagctga tccgcctgct cgtccagcag
    16921 caggtgatag tcgcggaaat gcggcccgct catgtgccag tggaaattct tggtcttcag
    16981 gtacagggcg aagacatcgg ccaggatggc attcattcca cccgcgatct cggccgaagc
    17041 tttcgtgccc agatcgctcg gggtgaccag cggcgcagcc cgccgacggt tcagctcctt
    17101 tcttttcctc tcgctcatgg ccatggcggc tcctcgaagt gatgggtcga ctctcccagg
    17161 ggtagccaat tgtcgcatcg ggattgaagc cgcgctcggt taaatgcacg ggtcaatgca
    17221 ccacgttgct catggcatcg ccgtcccgag cggtcatggc caatagctgc tgagcgccct
    17281 cgagacaggt gcattcgcgc tcgagtctga gataattgcg atcagctggg tggggacggc
    17341 aggggtcata ccctgccgtt tccttttggg ccagctgaac agcctacgag gatgtgtact
    17401 cgtgaatacg ctcgctgatt tcgccatcac gaacaaatgg cctgcccgct atccggagcg
    17461 gatccagctc tattcccttc cgacaccgaa cggggtgaag gccgcgatca tgctcgagga
    17521 gaccgggctc gcctacgagc ctcaccgggt caacttcgat acccaggatc aaacctcgcc
    17581 agagttcctg tcactcaatc ccaacaacaa gatcccggcc ctcatcgacc cgaacggccc
    17641 ggatggacga ccgctgccgc tgttcgaatc cggcgccatt ctcatctatc tggctgacaa
    17701 gaccggcaag tttctggcag gcgccggcgg ggatcgctac cagaccctgc aatggctgat
    17761 gtggcagatg ggcggcgtgg gaccgatgtt tgggcaggtc ggcttcttcc acaagtttgc
    17821 tggccggcag tacgaggaca agcgcccgct cgagcactac gtgacggagt cgaagcgcct
    17881 gctccgcgtc ctcaacgaac gcctggtgag ccgcacgtgg atcatgggcg atgcgtacac
    17941 aattgcggat atcgcgacct ttccctgggt tcggaatcta atgggcttct acgaggccgg
    18001 cgatctcgtc gggatttccg gctatcccca tgtcctgcgc gccctggaag cattcgttgc
    18061 gcgccccgcc gtagcgtcgg gcctcaccat tccgcgggag gagtgagatg tcggggaagg
    18121 ccgagccagc gctagatcga gcacaagagc gtccaacacg cgagcgcttc aggcgcctgc
    18181 ctgatggcgc cactcgccct gaaccacgat ctccgagcgg acggaattgg caatgtagca
    18241 ctgctcgtgc gcctcgtgat gcagccgggc cacttcggtc tcgctggggg cctggggacc
    18301 ggaaaagaca atcttagggg ccagcacgac cgaggtgatt gcctggcgtc cgcgcgagtc
    18361 cttgctcatc gtgccgacag cgaggtcctc gtatgcatcc actatcagtc gcgccttcgc
    18421 cgcgagatag agaaaggtca acatgtgaca ggaagagatc gccgcgatga gggcctcttc
    18481 cggatcgacg ttctcagctc tcgagtaggg cagcgggacc gaatggaccg cgctggaggc
    18541 gggcacgacg acaccgccgt cgaagtgcca ttcgtgtgcc cggctgtatc gggagtcgac
    18601 gaagcgctca tggtgtcccc gccgccatgt aatctgcgcc tggtaactcg ccatcccgaa
    18661 taccgccgtg gccattgccg ggtgctgccc gaccgaggag ctaggctacc accggggacc
    18721 ggtgtgggac atcgcgatgc gagcgtcaac gaccctggtg aatcaggtgg cgatcaaggc
    18781 gagccgtctg ccgggtcacg gcagagtctt catcgagtgg cgtcgctggc tcgcagatgg
    18841 cgttctgtcc gccaatcgat tgcgcgcgca tattgaacag ggtgtagtcg agacgcgcat
    18901 cggttcgccc gcccgcacag gccttgacca gcatcgcttc ggagtcgctg tagcgatcgg
    18961 tgcccaccac gaccaccgtg gcaatcatgc tggcgatcag gacgattcca agaacgatgc
    19021 ggctcgctgc gttcagcatg accctctgcc tccgtttgca gtgcggcttg gcggggcgac
    19081 agtgtcgagg ccccacgccg agtagaacgt aaacctcggg agaaagagga catccgccct
    19141 tcagtgctta tcgggcgcgc ggtagagctg gggacccaac acatgaacgt tactgaaaag
    19201 gagcgagacg ctccacgtcg cgtatggtca ccggcccttc gtcggacttg acattctcac
    19261 cgtcgatccg ctcctggtac gccttcgccg tcagcaacga gatcatgcca ttggggtac
//
LOCUS       DASRHN010000022         9094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1655567, whole genome shotgun sequence.
ACCESSION   DASRHN010000022 DASRHN010000000
VERSION     DASRHN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655567"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFB37_00760"
     CDS             <1..640
                     /locus_tag="VFB37_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZO20999.1"
                     /translation="YREGYSLDALMSEVAELVLALLSGTSAPLHVQRISYREAFLSQL
                     QLDPFTARAADLVEAATRTVGFHSVGRELARDELLELLMGAYIGPRLGQDALTFVHGY
                     PATQAALARLDSHDPRTALRFELYCRGIELANGFQELASSAEQRMRFEQDNDERRRRG
                     LPVIALDERLLAALEHGLAACAGVALGFDRLVMLATGCPRIDEVVPFPIERA"
     gene            740..1249
                     /locus_tag="VFB37_00765"
     CDS             740..1249
                     /locus_tag="VFB37_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZO21000.1"
                     /translation="MTFTGKRYNFHDKAGDYAQLASELEGLLQGEPDLIANAANTSAL
                     LFEALPDLNWVGFYFLRGAATRSELVVGPFQGKPACVRIPLGKGVCGSAAAKRSTIVV
                     PDVNEFPGHIACDAASRSEIVIPLIKESTLLGVLDLDSAKLARFDEADRQGLERLAAV
                     FVAASRVES"
     gene            1246..2139
                     /gene="rarD"
                     /locus_tag="VFB37_00770"
     CDS             1246..2139
                     /gene="rarD"
                     /locus_tag="VFB37_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HZO21001.1"
                     /translation="MSGTGRGFSATIAAFAMWGAFPLYFHALYQVSAFQVIAHRVLWS
                     CVFVLIWMGLRGELPVLRTVMGNRGALGRLVVTATLITINWTVYVWAVITGHVVEASL
                     GYFTGPLVNVLLGVVLLRERLTRVQWTAILIAAAGVTYLGVMAGRPPWIALTLAFSFG
                     TYGLLRKLVQVEALPGLAVETVVLVPFAVGYLLWCESAGSGAFTHLGPGIDALLIGSG
                     PLTAIGLFLFAYGTRRLPYSTVGILQYIAPTGQLLCGVLAFHEPFEKVRATGFALIWI
                     ALLLYATEGLRLSRKQQRAFA"
     gene            complement(2170..2739)
                     /gene="efp"
                     /locus_tag="VFB37_00775"
     CDS             complement(2170..2739)
                     /gene="efp"
                     /locus_tag="VFB37_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZO21002.1"
                     /translation="MASYTTSEIRGGMKVLLDGDPFAVVENEFVKPGKGQAFNRIKVR
                     NLRTGRTIERTFRSGESLEAADVQDTEMQYLYRDGDFWHFMVPESFEQYTADKTSVGE
                     SAQWLKDGVVCIVTLWNNVPLVVTPPPHIDLKVIETDPGLRGDTATGGQKPAKLETGA
                     VVRVPLFINEGEVLRIDTRTGEYVSRAKQ"
     gene            2901..4952
                     /locus_tag="VFB37_00780"
     CDS             2901..4952
                     /locus_tag="VFB37_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO21003.1"
                     /translation="MTEQNAVPLIVLSSTRDPVEAINSILRRAGQPVHCTWIPALRDL
                     ADALTQINPELLIHVVPVTEELKSAIAVRDQVAPTVPVIGIGEHADEARIGQALEAGA
                     RDFLSFGNPARLQAVMLRELRSFRLERALSTTLKSARDARRQLETVLQRSNDAIVQVQ
                     EGIVVDANPSWLELFGFAESLVGQPIMDLFDEATQPALKGALAACLQGRWSDHTLKVN
                     AVLADGSSLPVEVVLALGEHDGEPCVRLVVPSTPRDERKLAEDLTEVVRSDSATGFLH
                     RRELLEALRQRLANPAAGGMRYITIVKLDKFASVERDVGATASEEVLIEFAKLLKAAL
                     HPKEIVGRFGGVKFLILLERGNEHDVEAWGEQLLGRVQKHVFRVRDKTVTVTCTVGFS
                     VVAAGQANLDALIADALDANQKGRHRGGNQTVTSDKADADTRVQSYDKVWVKHIKAAL
                     MENRFRLVQQPVASLQGEDPGMFDVLVRMIDLQGKEVLPSEFMAAAERNDLLKNIDRW
                     VVGASLSFAAQRKPGCLFVRLSKDTTKDGTFVDWLDNHVRSSRAEPQRLCFQVTEEIA
                     SAYLAQVKALATVLRQSGFRFALDGFGSGRDPKGLLESVPLDFVKVDGAIVQGLTADQ
                     QLQQRVRHLVDQATKRQVQTIAERVEDANTMAILWQIGVQYIQGYFVNEPEQVVLRAE
                     R"
     gene            complement(4982..6541)
                     /locus_tag="VFB37_00785"
     CDS             complement(4982..6541)
                     /locus_tag="VFB37_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZO21004.1"
                     /translation="MTYRARKCRSGMLIGMALLSSCALSAVKIAAAAAAGTQEQQIHS
                     LQKALKDVLARLDQLADQNQQLMKRQEQLQAQVSQQQLALQRTARAQPPETVSALPDQ
                     SAAQTAAAAPASSPASAVPSPATSTAGTGGLPGAQPGGFGLAALSNVRLWGYGEIYYN
                     RPTHEPNRTQVDLARAVFGIGYHFDDRTEFNSEYEVEHAVASASDPGEFEVEQFYIDR
                     ELTDRVGLRAGLFLMPFGLLNEHHEPTNFYGVQRNFVETLIIPSTWREGGLSLHGDTE
                     LGLSWSAGLTTGVDLSKWQFAPEFPQYTTALQLEDNDVAPLQASHQELALANGRHLSQ
                     YLAVNYFGVPGLDVGGAITTGKAVPVPGSPGDPRVALWEGHARWTPGRFDLSGVYAQG
                     TISDVAAANRANPGSPNPIPSAFYGYFLQAAYGLWERGEYRLNPFVRWEHYDMGSRYS
                     GAAPVIPTGSVPLSDSPGDFGLWPLGRDRVWTVGANFYVTPHLVFKADYQWFDVNTEF
                     DRFDLGLGLNF"
     gene            complement(6681..8945)
                     /gene="parC"
                     /locus_tag="VFB37_00790"
     CDS             complement(6681..8945)
                     /gene="parC"
                     /locus_tag="VFB37_00790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZO21005.1"
                     /translation="MDNQELNFEGIERQPLRTFTEKAYLDYSMYVILDRALPALGDGL
                     KPVQRRITYSMSELGLSAASKHKKSARTVGDVIGKFHPHGDGACYEAMVLMAQAFAYR
                     YPIVDGQGNWGSQDDPKSFAAMRYTEAKLTRYADVLLGELAHGTVDWQANFDGTLEEP
                     VILPARLPNLLLNGASGIAVGMATDIPPHNLREVAAACIHLLEDPEATTAALMKHVKG
                     PDLPTGAEIISPRADLKEFYEKGVGAFKARATYEIEDGNIVITAFPHQVSGSRVLQQI
                     DEQWRAKKLPMVESYNDESDHENACRLVIVPRSNRVDLVELMNHLFATTDLERGYRVN
                     LNIIGLDGRPRVMGLKEILTGWLEFRLRTVTRRLQHRLEKVSRRLHILEGLLVAFLNL
                     DEVIRIIRREDEPKPVLMKRFKLSDEQAEEILNTRLRHLAKLEEMKIREEQKALAAER
                     EELEALLKSKARLKKLIAREIQEDADRHGDDRRTRIVEREAAQAIDETSLIPNEPVTV
                     VLSTGGWARQAKGHEIDPRTLSYKGGDAFRAIARGRSLQPAVFLDSTGRTYSLPAHSL
                     PSARGQGEPLSGRLNPPDGATFAGVMMGEPEDLWLLASDAGYGFTVRLKELITDRRAG
                     KTVLNVPTNCQALPPALVLSADSLLVAVSSEGKMLAFPVTDVPEMPRGKGNKLYDIPS
                     KRAASREEILKAVAIVPPAGKLILCTAEKQKVLSWSELKDYRGQRAQRGSVLPRGWRE
                     IERLQVELPADPKA"
     gene            complement(8954..>9094)
                     /locus_tag="VFB37_00795"
     CDS             complement(8954..>9094)
                     /locus_tag="VFB37_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21006.1"
                     /translation="RTRRLVQLTLEGKDNTDQLMDMLLAKKRAADRREWLEQNGNLAE
                     VL"
BASE COUNT         1503 a         2947 c         2946 g         1698 t
ORIGIN      
        1 gtaccgggag ggctattcgc tcgatgcgct catgagcgag gtcgccgagc tggtcctggc
       61 gctgctgagc ggaacgtccg ccccgctgca cgtgcagcgg atcagctacc gtgaggcttt
      121 cctgagccag cttcagttgg atccgttcac cgcgcgcgcc gccgacctgg tggaggcggc
      181 tacccgtacg gtcggcttcc actcggtggg tcgcgagctc gcgcgcgatg agctgctcga
      241 gctgctcatg ggtgcctaca tcggtccacg gctgggacag gacgcgttga ccttcgtcca
      301 cggttatccc gctacacagg cggcccttgc acgcctggat tcccacgacc cgcgaacggc
      361 cctgaggttc gagctgtact gtcgcggaat cgagcttgcc aacggcttcc aggagcttgc
      421 ctcctcggcc gagcaacgca tgcgcttcga gcaggacaac gatgagcgcc gccgcagggg
      481 cctgcccgtc atcgcgctcg atgagcggct gctcgcagcg ctcgagcacg gactcgcggc
      541 ttgtgccggc gtggccctcg gattcgaccg gctggtgatg ctggccaccg gctgccctcg
      601 gatcgatgag gtggtgccct ttccgatcga gcgcgcgtga gcgcgtgacc cgagcgccgt
      661 ggcgtcgctc ttccggggga ggcgtggcaa gcggttttcg cgtcacaatg catgctgcca
      721 actgcgcgct gagctttcca tgaccttcac gggaaaacgc tacaacttcc atgacaaggc
      781 cggcgactac gcgcagctcg ccagcgagct cgagggcctt ctgcaaggcg agccggacct
      841 gatcgcgaat gccgcgaaca ccagcgcgct gctgttcgag gcgctgcctg atctcaactg
      901 ggttggattc tacttcctgc gcggcgcggc gacgcgcagc gagctggtcg tgggcccgtt
      961 ccagggcaaa ccggcctgcg tgcgcattcc gttgggcaaa ggcgtgtgtg gaagtgcggc
     1021 agcgaagcgc tcgaccatcg tcgtgcccga cgtcaacgag tttccgggcc acatcgcctg
     1081 cgacgcggcc tcacgttcgg aaatcgtcat ccctctgatc aaggagtcta ccctgctggg
     1141 agtgctcgac ctcgacagtg caaagcttgc acgcttcgat gaggccgacc gtcagggtct
     1201 ggagcgtctc gccgcggtgt tcgtggccgc gagtcgcgtg gagtcatgag cggcacgggc
     1261 cgcggattct ccgccacgat tgccgcattc gcgatgtggg gcgcgttccc actctacttt
     1321 cacgcgctct atcaggtttc agcgttccag gtcatcgcac atcgcgtgtt gtggtcatgt
     1381 gtgttcgtgt tgatctggat gggcttgcgc ggggagctgc cggtgctgcg gacggtgatg
     1441 ggcaatcggg gcgcattggg ccgtctcgtg gtgacggcca cactcatcac catcaactgg
     1501 accgtctatg tctgggccgt gatcaccgga cacgtggtcg aggcgagcct cgggtacttc
     1561 accggcccgc tggtgaatgt gctgctcgga gtggtgctat tgagagagcg actcacacgc
     1621 gtgcaatgga cggccatcct cattgctgcg gcgggcgtga cctatctggg cgtcatggcg
     1681 ggccgtcccc cctggatcgc actgactctc gcattctcgt ttggaaccta cgggctgctg
     1741 cgcaagctgg tgcaggtcga ggcgctgcct ggcctggcgg tcgagaccgt ggtgctggtg
     1801 cccttcgcag tgggctatct gctgtggtgt gagagcgccg gatcgggtgc cttcacccat
     1861 ctggggccgg ggatcgatgc gctgctgatc ggcagcggcc cgctgaccgc catcggcttg
     1921 ttcctgtttg cttacggaac ccgccgactg ccctattcga ccgtgggcat ccttcagtac
     1981 atcgccccca ccggccagct cctgtgcgga gttctggcct ttcatgagcc ctttgagaag
     2041 gtgcgcgcga cggggtttgc gctgatctgg atcgcgctac tgctgtatgc gaccgaaggc
     2101 ctgcgtctct cgcgcaagca gcagcgcgcc tttgcttagg gtctggaggt gcggtctcga
     2161 ggcgcggtct tactgcttcg cgcgcgagac gtattcaccc gtgcgcgtat cgatgcgcag
     2221 cacttcgccc tcgttgatga agagcggcac ccgcactaca gcaccggtct cgagtttcgc
     2281 tggcttctgt ccaccggtcg cggtatcgcc gcgcagaccc ggatcggtct cgatgacctt
     2341 gaggtcgatg tgcggcgggg gtgtcacgac caggggtacg ttgttccaca gcgttacgat
     2401 gcagactacg ccatccttga gccactgcgc cgactcgccc accgaggtct tatcggcggt
     2461 gtactgctcg aaactctccg gcaccatgaa atgccagaaa tccccgtccc ggtagagata
     2521 ctgcatctcg gtatcctgga cgtcggccgc ctcgagagac tcgcccgagc ggaacgtgcg
     2581 ctcgatggtg cgcccggtcc tgaggttgcg gaccttgatg cggttgaacg cctggccctt
     2641 gccgggcttg acgaattcgt tctcgacgac ggcgaatgga tcgccatcga gcaggacctt
     2701 catcccgccg cgtatttcgc tggttgtgta gctggccatg gcgctgatcg ggaactgtcg
     2761 ggggacccac agggggccgc gtatgatagc ggcatcgacc cccacctgcc aggaaaagcg
     2821 aactggcgca tggggagccg cggcgggccg atgctagaat cccgcccgat ttttccgcac
     2881 ttttccggct ggagcccgca ttgaccgagc agaacgcggt accgctcata gtgctcagct
     2941 ccacgcgcga cccggtggag gcgatcaaca gcatcctgcg gcgcgcgggt cagccggtac
     3001 actgtacctg gattcccgcc ttgcgcgatc tcgccgatgc gctcacgcag atcaaccccg
     3061 agctgctgat ccacgtcgtc cccgtcactg aggagctgaa atcggcgatc gccgtgcggg
     3121 accaggtggc gcccacggtt cccgtgatcg gcatcggcga gcacgccgat gaagctcgca
     3181 ttgggcaggc cctggaggcc ggcgcgcgcg acttcctttc cttcggcaac ccggcgcgcc
     3241 tgcaggcggt gatgctgcgc gagctgcgct ccttccgcct ggagcgcgct ctcagcacaa
     3301 cactgaagtc cgcgcgggat gcgcggcggc aactcgagac ggtcctgcag cgatccaacg
     3361 atgcgatcgt gcaggtccag gaaggcatcg tcgtcgatgc caacccttcc tggctggagc
     3421 tgttcggatt tgccgagagc ctggtcggac agccgatcat ggacctgttc gatgaagcga
     3481 cccaacccgc gctgaaaggt gcgctcgcgg cctgtctgca ggggcgctgg agcgatcaca
     3541 cgctcaaggt caatgccgtg ctggccgatg gcagctcgct gccggtagag gtcgtcctgg
     3601 cgcttggcga acatgatggc gagccgtgcg tgcgcctcgt cgtgccctcg acaccgcgcg
     3661 atgagcgcaa gctcgcggaa gacctcaccg aagtcgtccg gtccgattcg gccacgggct
     3721 ttttgcatcg ccgcgagctg ctcgaggcgc tgcgacagcg cctggcgaac cccgccgcag
     3781 gcggcatgcg ctacatcacg atcgtcaagc tcgacaagtt cgccagcgtc gagcgcgatg
     3841 tcggcgccac ggccagcgaa gaggtgttga tcgagtttgc caaactgctg aaggctgcac
     3901 tgcacccgaa agagatcgtg gggcgcttcg gtggcgtgaa gttcctgatc ctgctcgagc
     3961 gcggcaacga gcacgacgtc gaggcctggg gtgagcagct tctcggtcgg gtccagaagc
     4021 acgtcttccg ggtgcgcgac aagaccgtga ccgtcacatg cacggtggga ttctcggtgg
     4081 tcgccgccgg ccaggcgaat ctcgatgcgc tgatcgccga tgcgctcgat gccaatcaga
     4141 aaggccggca tcggggcggc aaccagacgg tcacctcgga caaggccgac gccgataccc
     4201 gtgtgcagtc ttatgacaag gtctgggtca agcacatcaa ggcggccctc atggagaatc
     4261 gcttccggct ggtgcaacag cccgtggcga gcctgcaggg tgaggatccg ggcatgttcg
     4321 acgtgctggt cagaatgatc gacctgcagg gcaaggaagt gttgccctcg gaattcatgg
     4381 cggcggccga gcgcaacgat ctgctgaaga acatcgatcg ctgggtcgtg ggagcctcgc
     4441 tgtcgttcgc ggcgcagcgc aagcccggct gtctgttcgt gcgcctctcg aaggacacca
     4501 ccaaggacgg cactttcgtc gactggctcg acaaccatgt ccgctcgagc cgggcagagc
     4561 cgcagcgcct gtgctttcag gtcaccgagg agatcgcctc cgcgtatctc gcccaggtga
     4621 aggcgctggc caccgtgctg cgccagagcg gcttccgctt cgctctcgat gggtttggct
     4681 ccggacgcga cccgaaaggc ctgctggaga gcgtgccgct cgatttcgtg aaggtcgacg
     4741 gcgcgatcgt gcaaggcctc accgccgacc agcagctgca gcagcgcgtg cgccacctcg
     4801 tcgatcaggc caccaagcgg caggtccaga cgatcgccga gcgagtggag gatgccaaca
     4861 ccatggccat cttgtggcag atcggcgtgc agtacatcca gggctacttc gtgaacgagc
     4921 ccgagcaggt ggttctgcgc gcggagcgct gagcacaggt cctgcgcgcg gagcgctgag
     4981 gttagaaatt gagccccaat ccgaggtcga aacggtcgaa ctcggtgttg acatcgaacc
     5041 attgataatc ggccttgaac accaggtggg gggtcacgta gaagttcgcc cccacggtcc
     5101 acactcgatc ccgtcccagc ggccacagtc cgaaatcccc aggcgaatcc gacagcggca
     5161 ctgagcctgt aggaatgaca ggcgccgctc ccgagtaccg cgagcccatg tcgtagtgct
     5221 cccagcgaac gaacggattc aggcgatact caccccgctc ccacagccca taggccgcct
     5281 gcaggaagta gccgtaaaag gcggacggaa tcgggttcgg cgatcccggg ttggcccgat
     5341 tcgccgccgc cacatcgctg atcgtgcctt gcgcatacac gcccgataga tcgaatctgc
     5401 ccggtgtcca gcgcgcatgg ccttcccaca gcgcgacgcg cggatcccct ggggaacccg
     5461 ggacgggtac cgccttgccg gtggtgatgg cgccgcccac atcgagaccc ggaacaccga
     5521 agtagttgac cgcgaggtac tgcgacaggt ggcggccgtt cgcgagcgcc agctcctgat
     5581 ggctcgcctg cagcggcgcc acgtcgttgt cttccagctg gagcgcagtc gtgtactgag
     5641 gaaactccgg cgcaaactgc cacttcgaaa gatccactcc cgtcgtgagc cccgcgctcc
     5701 aactgaggcc cagctcggta tcgccatgca gactcaagcc tccctcgcgc caggtgctcg
     5761 gaatgatgag ggtctcgacg aagttgcgtt gcaccccgta gaagttcgtg ggctcgtggt
     5821 gctcgttcag caatccgaag ggcatcagga acaggccggc acgcagtccc acgcgatcgg
     5881 tgagctcacg atcgatatag aactgctcga cctcgaactc accgggatcg ctcgcggagg
     5941 cgacggcatg ctcgacctcg tactcggagt tgaactcggt gcgatcgtcg aagtgatagc
     6001 cgattccaaa caccgcccgc gccagatcga cctgcgtgcg gttgggctca tgcgtcgggc
     6061 ggttatagta gatctcaccg tagccccaca gccgcacgtt gctgagggcg gcgaggccga
     6121 acccgccggg ctgcgctccg ggcaatccgc ccgtgccagc cgtcgatgtc gccggagatg
     6181 ggactgccga tgccggtgac gacgcaggcg ccgcggcagc cgtttgcgct gcgctctgat
     6241 cgggaagtgc ggacacggtt tccggcggtt gcgcgcgcgc agtacgctgc agcgccagct
     6301 gttgctgcga gacctgcgcc tgcaattgct cctgccgctt catgagctgc tggttctggt
     6361 cggcgagctg atcgagtcgc gcgagaacat ccttgagcgc cttctgcagc gaatgaatct
     6421 gctgctcctg tgtgcccgca gcggcggccg cggcgatctt caccgcactc agcgcgcagc
     6481 tgctcagcag ggccatgccg atcagcatcc ccgaacgaca cttacgggct cgataggtca
     6541 tgagtcgtat cctcgggtca gcgcgccgcc ggagagaccg caggcctgct atacgcgaat
     6601 acgaatctta acgcgaatgg ttctcattcg ccaatgcgga cagattgtcc ccggagtgaa
     6661 ggtccagggg cgcgcttctc ttacgccttc gggtccgcgg gcaattccac ttgcaaccgc
     6721 tcgatctcgc gccagccccg cggcagcaca ctgccgcgct gcgcccgctg gccgcggtag
     6781 tctttcagct cactccagct gagtaccttc tgcttctcgg ccgtacacag gatgagcttg
     6841 ccggcgggcg gcacgatcgc tacggccttg agaatctcct cgcgggaggc ggccctcttc
     6901 gaaggaatgt cgtacagctt gttgcccttg ccgcgcggca tttcgggcac gtccgttacc
     6961 ggaaacgcga gcatcttgcc ttcgctgctg actgcaacga gcagcgagtc cgctgagagc
     7021 acgagcgccg ggggcagtgc ctggcagttg gtggggacgt tgagcacggt tttgcccgca
     7081 cggcggtcgg tgatcagctc cttcaaacgc acggtgaatc cgtagcccgc atcgcttgcg
     7141 agcagccaca ggtcctcggg ctcacccatc atcacgccgg caaacgtggc tccatcgggc
     7201 ggattgagcc gaccggagag tggctcgccc tgcccgcgcg cggagggcag ggagtgggct
     7261 ggaagcgagt aggttctgcc ggtgctgtcg aggaacaccg caggctgcag actgcggccg
     7321 cgcgcgatag cccggaaggc atcaccgccc ttgtacgaca gagtgcgcgg atcgatctcg
     7381 tgacctttgg cctggcgtgc ccacccgccg gtcgacagca cgacggtcac gggctcgttg
     7441 gggatgagag aggtctcatc gatcgcctgg gctgcttcgc gctccacgat gcgcgtgcgg
     7501 cggtcatcgc cgtgccggtc cgcatcctcc tggatctcgc gcgcaatgag cttcttcagt
     7561 cgggccttgc ttttcagcag cgcctcgagc tcctcgcgct cggccgcgag cgccttctgc
     7621 tcctcgcgga tcttcatctc ttcgagcttc gcgagatggc gcagccgcgt gttgagaatc
     7681 tcctccgcct gctcgtccga aagcttgaag cgcttcatca ggacgggctt gggctcgtcc
     7741 tcgcggcgga tgatgcggat cacctcatcg agattcagaa aagcgaccag cagcccttcg
     7801 agaatgtgca gccgcctcga gactttctcc agccggtgtt gcaggcggcg cgtgaccgtc
     7861 cgcaggcgaa attccagcca gccggtgagg atctccttca gacccatgac ccgcggacgg
     7921 ccgtcgagcc cgatgatgtt gagattgacg cgatagccgc gctcgaggtc cgtggtcgca
     7981 aacagatggt tcatcagctc gaccaggtcg acccggttgg agcgcggcac gatcaccagg
     8041 cggcaggcat tctcgtgatc ggactcgtcg ttgtagctct cgaccatcgg cagcttcttc
     8101 gcgcgccact gctcatcgat ctgctgcagc acgcggctgc ccgagacctg atgaggaaac
     8161 gccgtgatga cgatgttgcc gtcttcgatc tcataggtgg cgcgcgcctt gaaagcgccc
     8221 acgcccttct cgtagaactc cttcagatcg gctcgcgggg agatgatctc ggctccggtg
     8281 ggcaaatccg ggcccttgac gtgcttcatc agcgccgccg tggtcgcctc cggatcctcg
     8341 agcaggtgga tgcaggccgc ggcgacctcg cgcagattgt gcggcggaat gtcagtggcc
     8401 atgccgacgg cgatgccgga ggcgccgttg agcagcaggt tgggcaagcg cgccggcagt
     8461 atgaccggct cctcgagcgt accgtcgaag tttgcctgcc agtccaccgt cccgtgagca
     8521 agctccccca gcaaaacatc cgcataccga gtcagcttcg cctcggtata gcgcatggcg
     8581 gcgaaggact tcgggtcatc ctgcgagccc cagttgccct gcccgtccac gatcgggtac
     8641 cgataggcaa aggcctgcgc catcagcacc atcgcctcat aacaggcgcc atccccatgc
     8701 ggatggaatt tgccgatgac atcgcccacg gtgcgcgccg acttcttgtg cttgctggcc
     8761 gccgagagcc cgagctcgct catcgaatag gtgatccggc gctgcaccgg cttcagaccg
     8821 tcgcccagag caggcagcgc gcgatcgaga atgacgtaca tcgagtaatc gaggtaggcc
     8881 ttctcggtga acgtgcgcag cggctgccgc tcgatgccct cgaagttcag ttcctggtta
     8941 tccatcgtga tctctacagt acttccgcca ggttgccgtt ctgctccagc cactcgcgcc
     9001 ggtccgccgc gcgcttcttc gcaagcagca tgtccatgag ctgatcggtg ttgtccttgc
     9061 cttcgagcgt gagctgcacc agccgtcggg tgcg
//
LOCUS       DASRHN010000023         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1820955, whole genome shotgun sequence.
ACCESSION   DASRHN010000023 DASRHN010000000
VERSION     DASRHN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820955"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFB37_00800"
     CDS             <1..688
                     /locus_tag="VFB37_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HZO21007.1"
                     /translation="PTISAEVFALRPDYCALSVVADEVANSPRHPQVTALAEAVGASW
                     SRPVWAEAHLESWRAAYRAFGAKPQRTPSSAEALLKRLADDGHLPNLNAAVDLYNAIS
                     VGYAIPVGGENAGAYAGHPRLIRAAGHELFDTIKDGSEHAESVPAGEVVWCDDRGVTC
                     RRWNWRQGVRTRIDPSTRQMWFVLERLEPMPLAALIEAGQLLVATLSEMSPEAAITTA
                     LLTREGRIST"
     gene            complement(709..1473)
                     /locus_tag="VFB37_00805"
     CDS             complement(709..1473)
                     /locus_tag="VFB37_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZO21008.1"
                     /translation="MYDSENLSVRRYQPGTRMVAHEHAEPCFGVVMQGWFLERIGGEE
                     RHYARGCATFCPAGTVHSQDFGPLGARQILFRPDESWLGWLGGARSLRAATYTCSESS
                     ACLADRLLSELGQDDEFSAMACEGILLELVAAFGRTGAAARSREQTPRWLLRVRDFIH
                     DNAHLQLDMKRIAEVARRHEVHLAREFRRFFGLPIGGYARRLRAQEAARLLSSSRASL
                     TEVALCCGFASHSHLCRVFKAHYGLTPSQFRSRLGN"
     gene            1602..3665
                     /locus_tag="VFB37_00810"
     CDS             1602..3665
                     /locus_tag="VFB37_00810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO21009.1"
                     /translation="MKARARRLWRSLGIGILPAMALVSHAQDALTPAQALDYRRIADL
                     HFSPDVTQLAYLVLSYSDDYAPHLSVMDLRTQTVRELTPPGKSDRLPQWAPDGRALGF
                     LSTRAGKPQVYLLTSQLGDPVSLTDRKFGVTSFRWSPDAKSIAYLALEDTAPGTQSGP
                     QVADLDAGLARLWIADIASHTTHRLRTEGYRIDEFQWQDTGHILLVASNSPRTEEFND
                     ALYSLSVSDGSISLLERPPQPFGGLILSPDRKQLALRSTSANGPIARDLFVGTIGRDD
                     LGDASKSLDRAVSEVHWSTQPQLLVRAADGFYNRLYRLDRRGTATLIPLSLSVDAFDI
                     GRGGQLAFAGEDFSHLPEIWLRALDGHLTQLTHLQTGVLSTHLATAQIFRTSSFDGSQ
                     IESALLRPSARESAARAPLVLLVHGGPASNFTAGYGWEVAWGELLASHGYAVLLVNPR
                     GSNGYGEDFVKANRCDWGGGDYRDLMSVLDAVIAGGEVDANRLGIGGWSYGGEMSVWA
                     TTQTDRFKAAVAGASVFDQAAEFETESGPAADEWYFGTPWEHPEVFARNSPSTFIRNA
                     HTPVLILDGEEDTANPVGQSKGLYRALKHFGVETQMVLYAGEGHSPRQGTNNIDMFQR
                     ILDWYDAHLKATQSALAPIRSSASFLEMHGNGPARRFCPTPAFGRHRAPSANTTVNSC
                     PVNSC"
BASE COUNT          710 a         1231 c         1214 g          701 t
ORIGIN      
        1 cccgacgatc agtgccgagg tgtttgcact ccgacccgac tattgcgcac tgagcgtggt
       61 ggccgacgag gtcgccaact cgcctcgtca tccgcaggtc actgcgctgg ccgaagcagt
      121 tggcgcctcg tggtccaggc ctgtatgggc ggaagcccat ctggaatcgt ggcgcgccgc
      181 gtaccgggcc tttggcgcaa agccgcagcg aacaccgtcc tccgccgaag cattactgaa
      241 acgcctggca gacgatggac acctgcccaa cctcaacgcc gccgtcgatc tatacaacgc
      301 catcagtgtt ggatatgcga tcccggttgg aggtgaaaac gcaggcgcct atgccggcca
      361 tccgcgcctg attcgtgccg cagggcatga gctcttcgac acgatcaagg acggcagtga
      421 gcacgcggag tccgtccctg ccggagaggt agtctggtgc gatgatcggg gcgtcacctg
      481 ccggcgctgg aactggcgcc agggcgtgcg gacgagaatc gatccgagca cccgtcagat
      541 gtggttcgta ctggagcggc tcgagcccat gccattggcc gccctgatcg aggcagggca
      601 gctgctcgta gccactctct ctgaaatgag tcccgaagcc gccatcacga cggcgctgct
      661 gactcgggaa ggacggatca gcacctgaga gcctttcact gggcgcggtc agttgccgag
      721 gcgggatcga aactgcgagg gcgtcagccc gtaatgtgcc ttgaagacgc ggcacagatg
      781 agagtgactg gcgaatccgc agcaaagcgc tacttcggtg agggaggcgc gcgagctgga
      841 cagcagcctc gctgcctcct gagctcgcag ccgcctggcg tagccaccga tcggcaaacc
      901 gaaaaatcgt cggaactctc tcgccagatg gacttcatgt cgtcttgcga cctcggcgat
      961 gcgcttcatg tccagctgca aatgagcatt gtcatggatg aaatcgcgca ctctcaggag
     1021 ccagcgtggc gtctgctcgc gcgagcgggc cgccgcgccg gttctgccga aggccgcgac
     1081 cagttccaga agaatgcctt cgcaggccat tgcggagaac tcgtcatcct ggccgagttc
     1141 cgacagcagc ctgtcagcga ggcaggcaga ggactccgag caggtgtagg ttgccgccct
     1201 caggctccgt gcgcccccga gccatcccag ccaggattcg tcgggcctga agagaatctg
     1261 ccgcgcaccg agcggaccga aatcctggga atgcacggtg ccggcgggac agaacgtggc
     1321 gcatccccgc gcatagtgcc gctcttcccc gccgattcgc tcgagaaacc aaccctgcat
     1381 gaccacgcca aagcatggtt cggcatgctc gtgtgcgacc atcctggtgc ccggctgata
     1441 gcgccgaacg gacaagtttt cagaatcgta cattctctgc tccggcgcgg acgcgaccgc
     1501 ggcgctgacc acgctaaaac tgatcaaagc gcgctacctc ggtggaagac ggcaacgtgg
     1561 ctgtgcggtt agcttagctg aacgttagcg aggatcgtgg catgaaagcg cgtgcacgtc
     1621 gcctgtggcg ctcgttgggt atcggaatac tgccggccat ggccctcgtc agccatgcac
     1681 aagatgcact gacccctgcg caggcactcg actaccgccg gatcgccgat ctgcacttct
     1741 cgcccgatgt cacccagctc gcctatctcg tgctgagcta ttcggatgac tatgctccac
     1801 atctgtctgt catggatctg cgtacccaga cggtccgcga gctcaccccg ccgggaaagt
     1861 cagaccgact cccccagtgg gcgcctgatg gccgagcgct ggggtttcta tccacccgcg
     1921 caggcaaacc gcaggtctat ctcctgacat cgcagctggg cgatccggtg tctctcacgg
     1981 atcgcaagtt cggcgtgacg agcttccgct ggtcgcccga cgccaagtcg atcgcctacc
     2041 tcgccctcga ggacaccgcc ccaggcacgc aaagcggccc gcaggtcgca gatctcgatg
     2101 ccggcctggc gcgcctgtgg atcgcagaca tcgcctctca cacgacgcat cgccttcgca
     2161 cggagggcta ccggatcgat gaatttcagt ggcaggacac gggccacatt ctcctggtcg
     2221 ccagtaactc cccgcggacc gaggagttca atgatgccct gtacagcttg tcggtttctg
     2281 acggctccat cagcctgctt gagcgcccgc cgcaaccctt cgggggtctg atcctctcac
     2341 cagaccgcaa acagcttgcc ttgcgctcca cctccgcaaa tgggccgatc gcgcgggatc
     2401 tgttcgttgg cacgatcggg cgcgatgatc tgggggatgc ctcgaagtct ctggacagag
     2461 ccgtgagcga ggttcactgg agcactcagc cgcagctgct cgttcgtgcg gctgacggtt
     2521 tctacaatcg tctctatcgg ctcgaccggc gcgggacggc gacactgatc cctctctcgc
     2581 tttccgtgga cgctttcgat atcggcaggg gtggtcagct cgcgttcgcg ggggaggact
     2641 tttctcactt gccggagatc tggcttcgag ccctggacgg tcacctaaca cagctcacgc
     2701 atctgcaaac gggcgtcctg agcacacatc tcgcgacggc gcaaatcttc cggaccagca
     2761 gtttcgatgg aagtcagatt gagtcggcat tgctgcggcc atcagcgcga gagagcgccg
     2821 cgcgagcgcc gctcgtgctc ctggtgcacg ggggtcccgc atcgaatttc actgcaggct
     2881 atggctggga ggttgcctgg ggagagctgc tggccagtca cgggtacgca gtgctgctgg
     2941 tgaatccgcg cggctcgaac ggctacggcg aggatttcgt caaggccaac cgctgcgact
     3001 ggggtggagg cgactaccgg gatctgatga gtgtcctcga tgccgtgatc gcaggtggcg
     3061 aagtggatgc aaaccgcctt ggaatcggcg gctggtcgta cggaggcgag atgtcggtgt
     3121 gggccaccac tcagaccgat cgcttcaagg cggcggttgc gggtgccagc gtgttcgatc
     3181 aggcggccga gttcgagacc gagagcggtc cggcagcgga cgagtggtac ttcggaacgc
     3241 cctgggagca tccggaggtg ttcgcgcgca attcaccttc gacattcatt cgcaatgcgc
     3301 acactcctgt cctgatactc gacggggaag aagatacggc caatccggtc gggcagtcca
     3361 aagggttgta tcgggccctg aaacacttcg gcgtggagac ccagatggtt ctgtatgccg
     3421 gtgagggaca ctcgccccgc caaggcacca acaacatcga catgttccag agaattctgg
     3481 attggtacga cgctcacctg aaggccacgc aatccgccct ggcgcccatc cgcagctcgg
     3541 ccagcttttt ggagatgcat gggaatggac cggcgcggcg tttctgccct accccggctt
     3601 tcggacggca ccgggcgccg tcggcgaata caacggtaaa ttcatgtccg gtcaattcgt
     3661 gttgagaggc ggaagctgcg caactctcac cgcatgcggt gccggcatga gggctaccgc
     3721 cctgattgat cccggcagac gaccgagcgg cgcgtgactc gagacggccc tctcgagcgg
     3781 ccggcctcct gtccgctcac tccagcgccg cgatccgaca gcgcacgggc gcatcctcgt
     3841 tgaagtggca aaccgc
//
LOCUS       DASRHN010000024         6942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_8129, whole genome shotgun sequence.
ACCESSION   DASRHN010000024 DASRHN010000000
VERSION     DASRHN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8129"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2212
                     /locus_tag="VFB37_00815"
     CDS             <1..2212
                     /locus_tag="VFB37_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO21010.1"
                     /translation="KESQAQLEAQQAELEQSNAQLEEQAHLLARQRDELATAQSVLAQ
                     RAADLERANQYKSEFLANMSHELRTPLNSSLILAKILADNRGANLTAEQVKYAQTISS
                     AGHDLLAIINDILDLSKIEAGKVELTPETVLVARVTEPLLKTFQPVAQSKGLAFRIEA
                     APGAPDRLETDAQRLGQILRNLLSNAFKFTERGDVGLRVDSGPDGTIRFVVIDTGIGM
                     SEHQKGIIFDAFRQADGSTHRRYGGTGLGLTISRDLARLLGGEVEVESEVGKGSTFTL
                     TLPLAFSGPVGLPATAASRTSAERPPTRSLALGSDGSEAPRAAPASASHPVSRQVEDD
                     SERIKPDARLILIVEDDPAFATILRDLAHEQGFQCIVRYTGADALAAVATYQPKAILL
                     DMNLPDTSGLGILDQLKRDPRMRHIPVHIVSVSDRSQEALAMGAIGYALKPARREQLI
                     EALQRLEAKFSQGMRHVLVVEDDVRQSESIRELLQNGAVQISTAGNAGDALKQLRTTT
                     FDCVVMDLNLPDYSGYQLLRQMAEQEEVAFPPVIVYTGRSLSSEEEQELRRFSRSIIV
                     KDARSPERLLDEVTLFLHQVEATLPLERQRMLQAARDRDAALEQRRILVVEDDVRNIF
                     ALSSLLEPKGASIEIARNGREAIEALTRSSLPGGSAIDLVLMDIMMPEMDGLTATRVI
                     RERPEWQKLPIIALTAKAMKDDQEKCLAAGANDYIAKPLDVEKLLSLVRVWMPK"
     gene            2220..3047
                     /locus_tag="VFB37_00820"
     CDS             2220..3047
                     /locus_tag="VFB37_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZO21011.1"
                     /translation="MLSADSDIELQLLIEAIYRKYHYDFRCYAPASLKRRVTAAMARL
                     NCHSYSQLQHRVLHEPAAFPELLDHLTVPVSEMFRDPSYFHSLRAAVIPVLRTYPSLK
                     VWVAGCGTGEEVYSLAILLREEGLLGRTLIYATDINPQTLQKAATGVYDVQRIPSFTE
                     NHRRSGARSSLSEYYTAAYGRAVFDKSLRKHIVFSDHSLATDSVFAEVQLVSCRNVLI
                     YFNRDLQDRAIGLFRDALCRKGFLGIGSKESLRFSAHTDAFSDLIAADRIYQRRDPP"
     gene            3044..3667
                     /locus_tag="VFB37_00825"
     CDS             3044..3667
                     /locus_tag="VFB37_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZO21012.1"
                     /translation="MKGAALSGGRIDAVVIGASAGGIEALSVLLPALQPNMVVAFFVV
                     LHVPRERPSLLVEIFQRRCALPVREPEDKEPVAPGTVYFSPPDYHMLVEKERRIALSA
                     DEPVHFSRPSIDVLFESAADAYAERLLGVILTGANQDGASGLEAIHRAGGVTVVQEPE
                     SALMPFMTLSALQRAPADHVLSLERIADLLRTLAGPVPASEAQSAPQ"
     gene            3664..4776
                     /locus_tag="VFB37_00830"
     CDS             3664..4776
                     /locus_tag="VFB37_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZO21013.1"
                     /translation="MKRVTERRVKCLIVDDLQENQLALTALLAREDVEVLTARSGSQA
                     LELLLVHDVALAFIDVQMPEMDGFELAELMRGSERTRQIPLIFVTAGAREPHRLFKGY
                     ESGAVDFLFKPIEPHIVRNKADVFFQLYRQKQQLAQELRERSETLRMNEMLAAVLSHD
                     LRSPLNAVMTSAQLMLRLSDEQRVLDAALRIESSCKRMSRMIEDMLDLARARLAGGIP
                     VKRAPADLGALVERVSQEIRAASPGRRIELARVGDLQGEWDAERLAQVTSNLLCNALQ
                     HGEDGPPVLVDLDGRDSESVTLMVTNGGTIPPDLLPRLFDPFRGAERQSGRSEGLGLG
                     LYIVQQIVLAHQGSVEVRTETAHRTCFVVRIPRKAR"
     gene            complement(4773..6050)
                     /locus_tag="VFB37_00835"
     CDS             complement(4773..6050)
                     /locus_tag="VFB37_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZO21014.1"
                     /translation="MSAWTAEVAKRGARFPAEELRQQFPALARSAPFVFADNAAGAQI
                     PQRVLDAVNLHLLDRNVQRGGRYRHSIAVDAAIAHARASVAAFVNARRPEEIAFGMNA
                     TSFIRLISLAVGQSLAERNEIVLTDLDHEANVATWLVLERFGAKFRWWKMRSDGSLHV
                     EDLKPLLSSRTRLVACTLTSNALGSIVDVRAAAEIAHAVGAEVFLDGVHYAPHGLIDV
                     QAWGCDYLVCSGYKIFAPHMGFMWGRYESLSALPTFREDFIPDEPPGKIEAGTFIFEN
                     VAGMAAAVDYLAEIGREAMRTAGTAKPASLREAIRQGMEAIRAYEQTLSRELIGVLRE
                     SGAQIYGPETEARLAQRVPTIAFNMPGCSPRSVTEAASRADIGIRDGHMYSPRLMERL
                     NLSVDSGLVRVSLVHYNTLAEIHRFGEVLGKLR"
     gene            complement(6301..>6942)
                     /locus_tag="VFB37_00840"
     CDS             complement(6301..>6942)
                     /locus_tag="VFB37_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO21015.1"
                     /translation="VLALAIIVATIPWTATGVKVVAQSPFVSVFQYSGIAHAAGIMNF
                     VVISAALSSMNTNVYLCARMLFSLSRGSYAPRVLGRLSPNGSPLAAILISGACILVSA
                     GVSFFTPNAYAYLLSVALFDAMLVWVFILVSHLSFRRHHEPRTLPLRMPFFPWLQYLG
                     LAMLAAILITMGLDSNMYYSWLCGIPWLLVVSAGYLLWKLRHRPQRAQVEGAS"
BASE COUNT         1319 a         2220 c         2199 g         1204 t
ORIGIN      
        1 caaggaatcg caggcccaac tcgaagctca gcaggccgag ctcgagcaga gtaacgcgca
       61 gctcgaggag caggcgcacc tgctggcccg tcaacgcgac gaactggcca cggcacaatc
      121 cgtcctcgcc cagagagcgg ccgatctgga gcgggctaac cagtacaaga gcgagttcct
      181 ggccaacatg agtcacgagc tgcgcacccc gctcaactcc tcgctcatcc tcgcgaagat
      241 cctggccgac aaccgcggcg ctaatctgac ggcggagcag gtgaagtatg cccagacgat
      301 ttcctcggcc ggccatgacc tgctggccat catcaacgac atcctggacc tctcgaagat
      361 cgaggccggc aaggtcgagt tgacacccga gacggtcctc gtggcccggg tgaccgaacc
      421 gctgctcaag acgttccagc ccgtggcaca gagcaaagga ctggcattcc gtatcgaggc
      481 ggcgcccggt gcgcccgatc ggctcgagac cgacgcccag cgtctcggcc agatcctgag
      541 gaatctgctt tccaacgcgt tcaaattcac cgagcggggc gacgtcggcc tgcgcgtcga
      601 ttccggacct gacggcacga tacggttcgt cgtgatcgat accgggatcg gcatgtccga
      661 gcatcagaag gggatcatct tcgatgcctt ccgccaggct gacggcagca cgcaccgccg
      721 ctacggcgga acgggcctgg ggcttacgat ctccagggac ttggcgcgac tgctgggggg
      781 cgaggtcgag gtagagagcg aggtcggcaa aggcagcacg ttcaccctca cattgccgct
      841 cgccttctca ggccctgtcg ggttgcccgc aactgcagcg tcaaggacct ccgcggaacg
      901 gccgcccacg aggtcactcg ctctcggatc cgacggcagc gaagcaccgc gtgccgcacc
      961 cgccagcgca tcacaccccg tatcgcggca ggtggaagat gattccgagc gaatcaaacc
     1021 cgatgcgcgg ctcatcctca ttgtcgagga cgatccagcg tttgcgacga tcctgcgcga
     1081 tctcgcccac gagcagggct ttcaatgtat cgtgcggtac acgggtgctg acgcgctggc
     1141 ggccgtagcg acttaccaac ccaaggccat cctgctggac atgaacctgc cggatacctc
     1201 cggtctcggt attctcgacc agctcaagcg cgatccgcgc atgcgacaca tccccgtcca
     1261 catcgtatcg gtctcggatc gttcgcagga agctctcgcc atgggcgcga tcggctacgc
     1321 cttgaaacct gcgaggcgcg agcagctcat cgaggccctg cagcgtctcg aggcgaaatt
     1381 ttcacagggc atgcggcacg tgctggtcgt ggaggacgac gtgcgacaga gcgagagcat
     1441 ccgggagctg ctgcagaatg gcgccgtgca gatcagtacg gccggcaacg ccggcgatgc
     1501 cctcaagcag ctgcgcacga caaccttcga ctgcgtggtg atggatctca acctgccgga
     1561 ctacagtggc taccagcttc tgcgtcagat ggccgagcag gaggaagtgg cctttcctcc
     1621 ggtcatcgtg tacacgggac gttcattgtc ctccgaggag gagcaggagc tgcgccgctt
     1681 ctccaggtcc atcatcgtca aggacgcccg ctctccggag cgtctgctcg atgaggtcac
     1741 tttgttcctg caccaggtgg aagcaacgct gccgctcgag cgccagcgaa tgctgcaggc
     1801 agcccgcgac cgggatgccg cgctcgaaca gcggcgcata ctcgtggtcg aggatgacgt
     1861 tcgcaacatc tttgcgctct cgagtctgct ggagccgaag ggtgcgagca tcgagatcgc
     1921 ccgcaatggc agggaggcca tcgaggcgct gacacgcagc tcgcttcccg gcggcagtgc
     1981 catcgacctc gtgctgatgg acatcatgat gcccgagatg gatggcctca cggccacgcg
     2041 ggttatccgc gagcggcccg agtggcagaa actcccgatc atcgcgctga ccgccaaagc
     2101 gatgaaggat gatcaggaga agtgtctggc cgccggcgcc aacgactaca tcgccaagcc
     2161 tctggatgtc gagaagcttc tctccctggt gcgggtctgg atgcccaagt aaggttcaca
     2221 tgctcagcgc ggacagcgac atcgagctgc agcttctgat cgaggcgatc tatcggaagt
     2281 atcactacga tttccgctgc tacgcgcccg cctcgctcaa gcggcgcgtc acggcggcca
     2341 tggcgcgcct gaactgccac tcgtattcgc agctgcagca ccgggtgctg cacgagcccg
     2401 cggcatttcc cgagctgttg gatcacctga cggtcccggt cagcgagatg ttccgcgatc
     2461 cgagttattt ccattcgctg cgcgcggcgg tcattccggt actgcgaacc tatccctcgc
     2521 tgaaagtctg ggtcgccgga tgcggcacgg gcgaggaggt gtactctctc gccatcctgc
     2581 ttcgcgaaga aggcctgctc gggcgtacgc tgatctatgc aaccgacatc aatccgcaaa
     2641 ctctgcagaa ggctgcgacg ggtgtctacg atgtgcagcg catacccagc ttcaccgaga
     2701 accaccgcag atcgggcgca cgctcatcac tgtcggagta ctatacggcc gcatacggcc
     2761 gcgcagtgtt cgacaagtcc ctgcgcaagc acatcgtatt ctccgatcac agcctggcga
     2821 ccgatagtgt cttcgccgag gtgcagctcg tgtcctgccg caacgtgctg atctacttca
     2881 atcgcgattt gcaggatcgg gcgattggcc tgttccgcga tgcgctgtgc cgcaaaggct
     2941 ttctcggcat cggctcgaag gagtcgctgc gattttcagc gcacacggac gctttctccg
     3001 atctcatcgc ggcggatcgc atctatcagc gccgggatcc gccgtgaaag gcgcagcgct
     3061 gtccggtggc cgcatcgatg cggtcgtgat tggtgcctcg gccggaggca tcgaggcgct
     3121 ctcggtgctg ctgccggccc tgcagcccaa catggtcgtt gcgttcttcg tggtgctgca
     3181 cgtgccgcgc gagcggccga gtctgctggt agagatcttc cagcgccgct gcgcactgcc
     3241 agtgcgcgag ccggaggaca aggagccggt cgcccccggc accgtctact tctcgccgcc
     3301 cgactatcac atgctcgtgg aaaaggagcg gcgcattgcc ctttcggcgg acgagcccgt
     3361 gcatttctca cggccatcga tcgacgtgct gttcgagtcc gctgccgatg cctacgctga
     3421 gcgtctgcta ggtgtcattc tcaccggcgc gaaccaggac ggcgcttccg ggctcgaggc
     3481 gatccaccgc gccggcggag tcaccgtggt gcaggagccc gaaagcgcgc tgatgccgtt
     3541 catgaccctg tcggccctgc agcgcgctcc ggcggaccac gtcttgtcac tggagaggat
     3601 agcggatctg ttgcggacgc tcgccggacc ggttcccgcg agcgaagcgc agtcagcacc
     3661 gcaatgaaac gagtgacgga gcgccgcgtg aagtgtctga tcgtcgatga tctgcaggaa
     3721 aaccagcttg cgctgacagc ccttctcgcg cgggaggacg tcgaggtgct caccgcccgt
     3781 tccgggtcgc aggcactcga gctgctgctg gtgcatgatg tggccctggc gttcatcgac
     3841 gtgcagatgc cggaaatgga cggcttcgag ctggccgagc tgatgcgcgg cagtgagcgc
     3901 acgcggcaga ttcccctcat cttcgtcact gctggcgccc gcgagccaca ccggctgttc
     3961 aagggatacg agagcggcgc ggtggacttc ctattcaagc cgatcgagcc gcacatcgtg
     4021 cgcaacaagg ccgacgtctt cttccagctc taccgacaga agcagcaact ggcacaggag
     4081 ctgcgcgagc gcagtgaaac gctgcgcatg aacgagatgc tggctgccgt gttgagtcat
     4141 gatctgcgca gcccgctgaa cgcggtgatg acctcggccc agctgatgct gcgcctttcc
     4201 gacgagcagc gcgtgctcga tgccgcgctg cggatcgagt cgagctgcaa gcgcatgagc
     4261 cgcatgatcg aggacatgct tgacctggcg cgcgcgcgcc tggcaggcgg catcccggtg
     4321 aaacgcgcgc cggccgatct cggcgcgctg gtcgagaggg tgtcgcagga aatcagggca
     4381 gcgagtcccg gacggcgcat tgagctggcg cgcgtcggcg acctgcaggg cgagtgggat
     4441 gccgagcgcc tggcgcaggt gacatcgaat ctcctctgca acgcacttca gcacggtgaa
     4501 gacgggccgc cggtgctcgt ggatctcgat ggtcgcgatt ccgaaagcgt aaccttgatg
     4561 gtgaccaacg ggggcacgat cccgccggat ctgctgccgc ggctgttcga tccattccgg
     4621 ggtgcggagc ggcaaagcgg gcgcagcgag ggcctcgggc tcggcctcta catcgtgcag
     4681 cagatcgttc tggcccacca gggcagcgtc gaagtgcgca ccgaaaccgc ccaccgcacc
     4741 tgcttcgtgg tcagaatccc gcgcaaggcc cgctagcgga gcttcccaag cacctcgccg
     4801 aagcggtgaa tctcggccag agtgttgtaa tgcacgagcg agacccgcac caggccgctg
     4861 tcgactgaca gattcaggcg ctccatgagc ctgggcgaat acatgtgacc atcgcgaatg
     4921 ccgatatctg cccgggaagc cgcctcggtc acggaccgag gcgaacagcc aggcatgttg
     4981 aaagcaatgg taggtacccg ctgggcgagg cgcgcttcgg tctccggtcc atagatctgc
     5041 gcgccgcttt cacgcagaac cccgatcagc tcgcgcgata gagtctgctc gtaggcgcga
     5101 atcgcctcca tgccttgccg gatggcctcg cgcagcgagg caggcttcgc cgtcccagcc
     5161 gttcgcatcg cctcgcgccc tatctcggcc aggtaatcca ccgccgcagc cattcccgcg
     5221 acattctcga agatgaacgt gcctgcctcg atcttgccgg gtggctcatc cgggatgaag
     5281 tcctcgcgaa aggtcggcaa cgcgctcaac gactcataac gaccccacat gaaacccatg
     5341 tgcggagcga agatcttgta tccggagcag accaggtagt cacaacccca ggcctgaaca
     5401 tcgatgagtc cgtggggggc gtagtgcacg ccgtcaagga atacctccgc tcccacggcg
     5461 tgcgcgatct ctgcggctgc tcgtacatcg acgattgagc ccagcgcgtt ggaggtgagc
     5521 gtgcaggcca ccaacctggt gcgcgatgaa aggagcggct tgaggtcctc cacgtgcaat
     5581 gagccatcgg agcgcatttt ccaccagcga aactttgcgc caaagcgttc cagcacgagc
     5641 caggtggcca cgttggcttc gtggtccagg tcggtgagca cgatctcgtt gcgctccgcc
     5701 aggctctggc caaccgcgag actgatcagc cgaatgaacg aagtcgcgtt cattccgaag
     5761 gcgatctcct ccggccgccg cgcgttcacg aaagccgcaa cgctcgcgcg agcgtgagca
     5821 atggcggcat ccaccgcgat gctgtgccga tagcggccgc cgcgctgtac gttgcggtcc
     5881 agcaaatgca gattgacggc atccagtacg cgctgcggaa tctgggcgcc ggcggcattg
     5941 tcggcgaaca cgaagggcgc ggaccgcgcc agcgcgggaa actgttgtcg taactcctcg
     6001 gctggaaacc gggcaccccg cttcgcaacc tcggcagtcc atgcgctcat ttcgctctcc
     6061 gctcgttcac actctcgggc tcgtttgatg ataacggagc cactcgtagc cgcagccgct
     6121 gaattaggcc cgccgcacta ggtgcagtgg gaagcttcgg caggcgcaga ggggccggcg
     6181 tcagcgagca tgatggattc gcttcggcgt tattttatct atttataatc aattgattat
     6241 caaactttca tgcatttatt tcgagacggc ttcccagcac gcgcgtcgac gggcgcccac
     6301 tcatgaagcc ccctcaacct gcgcccgctg cgggcgatgc cgcagcttcc agaggagata
     6361 gccggccgaa accacgagca gccacgggat gccgcagagc cacgagtagt acatgttgga
     6421 atccagtccc atggtgatga ggatggcagc cagcatcgcc agcccgaggt actgcagcca
     6481 cggaaagaac ggcatgcgca gtggcagagt gcgcggctcg tggtggcggc ggaagctcag
     6541 atggctgacg agaatgaata cccagacgag catggcgtcg aacagcgcaa cgctcagaag
     6601 gtacgcatag gcgttcggcg tgaagaacga cacgcctgcg gagacgagga tgcacgcccc
     6661 tgagatgagg atggctgcga gcgggctgcc attaggactc agcctgccca gcactcgagg
     6721 cgcgtaactg ccgcgtgaca gcgagaacaa catccgtgca cacagataca cattggtgtt
     6781 catgctggac agtgcggcgc tgatcaccac gaaattcatg atgccggcgg cgtgagcgat
     6841 gccggagtac tggaagacgc tcacgaacgg gctctgggcg acgaccttga ccccggtggc
     6901 ggtccaggga atcgtcgcca cgatgatggc aagggcgagc ac
//
LOCUS       DASRHN010000025         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_174089, whole genome shotgun sequence.
ACCESSION   DASRHN010000025 DASRHN010000000
VERSION     DASRHN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174089"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFB37_00845"
     CDS             <1..198
                     /locus_tag="VFB37_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21016.1"
                     /translation="SHCGPVGVQGYLLAYPVEMHEAESESRAAAARARTVLEVRAESS
                     AQESSATPLVFVSAGARRRPS"
     gene            complement(195..878)
                     /locus_tag="VFB37_00850"
     CDS             complement(195..878)
                     /locus_tag="VFB37_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HZO21017.1"
                     /translation="MAAMKTELTSDEARIIGCLIEKQLTTPDQYPLSLNALVNACNQK
                     SNRDPVVAFDEPTVQHTVDALVRKHLVVERSGFGSRVPKYQHRFCNTEFGTLKFDPQE
                     LAVVCELLLRGPQTPGELRSRAARMAPFQDVSETEATLTKLMQREDGPFVVRLAREPG
                     RRESRYAHLFSGPPPAALSDPTPFTDARSAPPEAALPASDHEARLARVEEEVRSLRAQ
                     LEELKGRSG"
     gene            complement(866..1948)
                     /locus_tag="VFB37_00855"
     CDS             complement(866..1948)
                     /locus_tag="VFB37_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZO21018.1"
                     /translation="MVPRSEPSEPIVIGHRGACGYVPEHTLTSYFIAMQLGADYVEPD
                     LVMTGDGVLIARHENEIGGTTDIAQHPEFAGRRTTKSIDGTAVTGWFTEDFTLAELKT
                     LRARERIPQVRSANTRFDGQFQIPTLEEILALLAAVQQQREAAARELGRTVPRRIGIY
                     PETKHPTYFAGLGLAMEELLVGALHRHGFSGPKGLACIQCFEVSNLKAMRAMTELPIV
                     QLLEGEGAPYDFVARGDRRTYADLIQPQGLAEIATYANAIGPYKEMLIPRGSDGTLSS
                     PTSLMAEAHAQGLQVHAWTFRAENQYLPSNLRQGAAHEPGELGDLQSELLPYLQAGLD
                     GFFTDQPDIGVRARDAFVSRRLSWRP"
     gene            2154..4043
                     /gene="glgB"
                     /locus_tag="VFB37_00860"
     CDS             2154..4043
                     /gene="glgB"
                     /locus_tag="VFB37_00860"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZO21019.1"
                     /translation="MLTEQDIYLFREGTHFRSYDKLGAHPTTTAAGEQGTEFAVWAPN
                     AASLSVVGDFNGWNPRANPLDPRKDSSGIWSGFIRGVRPGALYKYHIESRHAGYTVDK
                     SDPFAFRCEVPPRTASVVWDLSYEWGDAEWMRNRARANALDAPQAVYEVHVGSWRRVP
                     EEHDRSLSYRELAHALADYVTEMGFTHVELMPIMEHPFYGSWGYQVTGYFAPTSRYGT
                     PQDFMYFVDYLHRRGIGVILDWVPSHFPADEHGLGYFDGTHLYEHADPRQGFHPEWSS
                     SIFNYGRAEVRDFLGSSAQFWLDKYHVDGLRVDAVASMLYLDYGRRAGEWIPNRFGGH
                     ENLDAVEFLKQLNLGLYRDHSDTQTVAEESTAWPMVSRPTYLGGLGFGLKWNMGWMHD
                     TLQYFRTDPLLRKYQHSALTFSLWYAFTENFVLPLSHDEVVHGKGSLFGKMSGDEWQK
                     YANLRLLLGYMWTHPGKKLLFMGGEFGQVREWTHDCSLEWHVLQYANHAGVQHWLRDL
                     NHFYRHRPSLYELDFSPDGFQWIDCNNADASVLVYLRKGRSPGDFTLVALNFTPIPRE
                     NYQIGVPRGGIWRERLNSDAREYWGSGLGNFGAIEAAPLPSHGHFHSLSLRLPPLGAL
                     VLTPE"
     gene            4067..>4405
                     /locus_tag="VFB37_00865"
     CDS             4067..>4405
                     /locus_tag="VFB37_00865"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HZO21020.1"
                     /translation="MSDVSKVRVVIENITPRVDCGRFPIKRTVGERVVVEADAFTDGH
                     DAVTVMLMHRHAASEQWHRSLMRPLGNDRWQGSFRVPSLGEYQYTVSAWVDHFESWRQ
                     GLLKKYEAGQD"
BASE COUNT          725 a         1436 c         1448 g          796 t
ORIGIN      
        1 tcgcactgcg gaccggtggg cgtgcagggc tatctgctgg cctacccggt cgagatgcac
       61 gaggcggagt cggaatcacg cgctgcagcg gcgcgggcgc gcacggtcct ggaggtcagg
      121 gcagaatctt ccgcgcagga aagcagcgcg actccgctgg tgttcgtctc ggccggtgcc
      181 cgccgccgcc cgagctagcc gctacggcct ttcagctcct cgagctgcgc acgcaagctg
      241 cgcacttcct cctccactct cgccagacgc gcctcgtggt ccgaggccgg caaggctgcc
      301 tcaggcggtg cgctacgggc atcggtgaaa ggggtcgggt ccgacagcgc agcgggcggc
      361 ggcccgctga agaggtgcgc atagcgggac tcgcgcctgc ccggctcgcg cgccaggcgg
      421 accacgaacg ggccatcttc ccgctgcatc agctttgtca gggtcgcttc cgtctccgac
      481 acatcctgga agggcgccat ccgcgcggct cgactgcgca gctcgccggg agtctgcggc
      541 cctcgcagca gcagctcgca cacgactgcc agctcctgcg ggtcgaattt cagcgtaccg
      601 aactccgtgt tgcagaagcg atgctgatac ttcgggacgc gactgccgaa cccggagcgc
      661 tccaccacga gatgttttct cacgagcgca tcgaccgtgt gctgcacggt aggctcgtcg
      721 aaggcgacca ccggatcgcg gttgcttttc tgattgcacg cattcaccag cgcattgagc
      781 gacagggggt actggtcggg cgtggtcagc tgcttctcga tcaggcagcc gatgatgcgc
      841 gcttcgtcgc tcgtgagctc ggtcttcatg gccgccatga taagcgccgg ctcacgaagg
      901 cgtctcgcgc ccgcacgccg atgtccggct gatcggtgaa gaacccatcg agcccggcct
      961 gcagatacgg cagcagctcg gactgcagat cgccgagctc gcccggctca tgggccgcgc
     1021 cctggcgcag gttcgagggc aggtattgat tctcggcgcg gaaggtccag gcgtgcacct
     1081 gcaggccctg cgcatgcgcc tctgccatga gcgaggtcgg cgagctcaag gtcccatcgc
     1141 tgccgcgcgg gatcagcatc tccttgtagg gtccgatcgc attggcgtac gtggcgatct
     1201 cggcaagccc ctgcggctgg atgagatcgg catacgtgcg ccggtcaccg cgcgcaacga
     1261 aatcatacgg cgcgccctca ccctccagca gctgcacgat cggcagctcg gtcatggcgc
     1321 gcatcgcctt gagattcgag acctcgaaac actggatgca ggcaagacct ttcgggccgc
     1381 tgaaaccgtg gcggtgcagg gcgcccacca gcagctcctc catggcaagg ccgagacctg
     1441 cgaaataggt gggatgtttg gtctcgggat aaatgccgat cctgcgcggg acggtccgcc
     1501 ccagctcgcg tgccgccgct tcgcgttgct gctggacagc ggccagcagc gcaagaatct
     1561 cctccagcgt cggaatctgg aactgcccgt cgaagcgtgt gttggcggac ctcacttggg
     1621 gaatgcgctc gcgcgcccgc aacgtcttca gctccgcgag cgtgaaatcc tcggtgaacc
     1681 agcccgtcac cgccgtaccg tcgatgctct tggtggtgcg acgcccggcg aactccggat
     1741 gctgggcgat gtcggtcgtg ccgccgatct cgttttcgtg ccgcgcgatg agcaccccgt
     1801 cgcccgtcat caccagatcg ggctcgacgt agtccgcgcc gagctgcatc gcaatgaaat
     1861 aggacgtgag cgtgtgctcc ggcacatagc cgcaggcgcc gcggtgaccg atgacgatcg
     1921 gctcgctggg ctcggagcgt gggaccacgt tagtgagtgt ggggcatggt gatggcgccg
     1981 atcccatgcg ggtccggcgc ggtattccag catcaagacc ggattgaggc aacagtggcg
     2041 gccgcggtcc tcgccgccgc gcgcgcgcag tcggccagct gccgcgcgtg cacaatcgag
     2101 ccggggcgcg cttttgggcg cgcagtgggc atgcctcttg ctagactggg gccgtgctga
     2161 cggaacaaga catctatctc tttcgcgaag gcacgcactt ccgctcgtat gacaagcttg
     2221 gcgcccatcc caccacgacg gccgcggggg agcagggcac ggagtttgcc gtctgggcgc
     2281 cgaatgcggc ctcgctgtcg gtagtcgggg acttcaacgg ctggaatccg cgcgccaatc
     2341 cgctcgatcc ccgcaaggat tcctcgggca tctggtccgg gttcatccgc ggtgtgcgcc
     2401 cgggagctct gtacaaatat cacatcgaat cgcggcacgc cggctatacg gtcgacaaga
     2461 gcgacccgtt cgcctttcgc tgcgaagtcc cgccgcgcac cgcttcggtc gtatgggacc
     2521 tctcgtacga gtggggtgat gcggaatgga tgcgcaaccg ggcgcgagcc aacgcgctgg
     2581 acgcgccgca ggcggtctat gaggtgcacg tcggctcgtg gcgacgcgtg cccgaagagc
     2641 acgaccgctc actgagctat cgggagctcg cgcacgctct cgccgactac gtgaccgaga
     2701 tgggtttcac gcacgtcgag ctgatgccga tcatggagca tccgttctac ggctcctggg
     2761 gctatcaggt caccggttac ttcgcgccga cctcccgcta tggcacgccg caggacttca
     2821 tgtacttcgt cgattacctg catcggcgcg gcatcggcgt gatcctcgac tgggtcccgt
     2881 cgcacttccc ggcggatgag catgggctcg gatacttcga cggcacgcac ctttacgagc
     2941 atgccgatcc gcggcagggc tttcatccgg agtggagcag ctccatattc aactatgggc
     3001 gcgccgaggt gcgcgacttt ctcggcagca gcgcacagtt ctggctcgac aagtaccacg
     3061 tggacggcct gcgggtggat gccgtggctt ccatgttgta cctcgattac gggcgccgcg
     3121 cgggcgagtg gattcccaac cgcttcggcg gtcacgagaa tctcgatgcc gtcgagtttc
     3181 tcaagcagct caatctcggc ctctatcggg atcactccga cacccagacc gtcgccgagg
     3241 aatccacggc gtggccgatg gtctcgcgcc cgacctacct cggcggcctc ggcttcggcc
     3301 tcaagtggaa catgggctgg atgcacgaca cgctgcagta cttccgcacg gatccgctgc
     3361 tgcgcaagta tcagcacagc gcgttgactt tcagtctctg gtacgccttc accgagaact
     3421 tcgtcctgcc gctttcgcac gatgaggtcg tgcacggcaa gggctcgctg ttcggcaaaa
     3481 tgagcggcga tgagtggcag aagtatgcga acctgcggct gctgctcggt tacatgtgga
     3541 cccacccggg caagaagctg ttgttcatgg gcggcgagtt cggccaggtc cgcgaatgga
     3601 cgcatgactg cagcctcgag tggcacgtgc tgcagtacgc gaaccatgca ggtgtgcaac
     3661 actggttgcg tgacctgaac catttctatc gccacaggcc ctccctgtac gagctggact
     3721 tctcgcccga tggcttccag tggatcgact gcaacaatgc ggacgcgagc gtgctggtgt
     3781 atctgcgcaa gggccggagc ccgggcgact tcacactggt tgcgctcaac ttcactccca
     3841 tcccgcgcga gaactatcag atcggcgtgc cgcgcggtgg aatctggcgc gagcgcctca
     3901 acagcgatgc gcgcgagtac tggggcagtg gcctgggcaa cttcggcgcc atcgaggccg
     3961 cgcccctgcc ttcgcatgga cacttccatt ctctcagcct gcgcctgccg cccctcgggg
     4021 cgctggtgtt gactcccgag tagcgcgccg ctcgccggtg cgcaacgtga gtgacgtgag
     4081 caaggtccgc gtcgtcatcg agaacatcac gccgcgggtt gactgcggcc gctttcctat
     4141 caagcgcacg gttggtgaaa gggtcgtggt cgaagcggat gcctttacgg acgggcacga
     4201 tgcggtgacc gtgatgctca tgcatcggca cgcggcctcc gagcaatggc accgctcgct
     4261 gatgcgaccg ctcggcaatg accgctggca gggcagtttc cgcgtgccct cgctcgggga
     4321 ataccaatac acggtctcgg cctgggtgga tcacttcgag agctggcggc aggggctgct
     4381 gaagaaatac gaggccggcc aggac
//
LOCUS       DASRHN010000026         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_997304, whole genome shotgun sequence.
ACCESSION   DASRHN010000026 DASRHN010000000
VERSION     DASRHN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997304"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VFB37_00870"
     CDS             <1..1266
                     /locus_tag="VFB37_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21021.1"
                     /translation="DRTSTTLQWSMHMFRTAWALTFCALLAARVYAWDQDAGALPEGA
                     GKQIAASKCLGCHDAGRLAAPGYTRAGWQSVIGRMVKMGAQVEPAQIPALSDYLARSF
                     PERPEPAARLIPGSVRVSFQEWAVATPGAFPHDPLATADGAIWYTGQHASVLGRIDPA
                     SGAIKEYPTRTADSGPHGLTADAAGSIWFTANSAGYIGKLDPGTGKITEYRMPDARAR
                     DPHTPIFDRHGILWFTVQGADMVGRLEPSTGNVKLVSVPIPHALPYGIVISSTGVPFF
                     AEFGSNRLASIDPHTLTIHEYVLPNSGSRPRRIAISSDDVLWYSDYARGCLGRFDPKS
                     GAAREWPSPGGPDSRPYAITALQDVLWYSESGVSPNTLVRFDPRSEKFQTWAIPSGGG
                     VVRNMMPAHDGTLVLAESGAGKLARVTIE"
     gene            complement(1314..1805)
                     /gene="msrB"
                     /locus_tag="VFB37_00875"
     CDS             complement(1314..1805)
                     /gene="msrB"
                     /locus_tag="VFB37_00875"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZO21022.1"
                     /translation="MSNFDLTPPSDTQRREITDGLDRNERRILLEHGTEAAFCGVFLD
                     NKKDGIYTCRFCGLPLFRSSAKFDSGTGWPSFFRPYDEAHIERLRDTSYGMIRVEEVC
                     ARCGSHLGHVFTDGPPPTGERHCLNSVSLGFTERGAPLPDVLHRGAPEGSPDAAPPRQ
                     STE"
     gene            1881..2651
                     /locus_tag="VFB37_00880"
     CDS             1881..2651
                     /locus_tag="VFB37_00880"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZO21023.1"
                     /translation="MFTRAIVRPPGASYAAGLTSSTEGVPDLGRAIAQHHEYCNALRE
                     CGLSVHALQPDERYPDGTFVEDTAVISGAMAVVTRPGAPSREGEIQGLAALLQGFGLS
                     VEEITAPGSVDGGDICQIEKHFLIGLSHRTNAEGARQLARILERYGYSSSEIDIRNNK
                     ALLHLKSGLSYLGENRLLVVPDLIVPEQLSRYQLISVSAEEGYAANCVRINDRVLVAA
                     GYPRLSASLERLGYSALALPMSEFRKMDGGLSCLSLRF"
     gene            complement(2656..>3258)
                     /locus_tag="VFB37_00885"
     CDS             complement(2656..>3258)
                     /locus_tag="VFB37_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZO21024.1"
                     /translation="AICGAAAVLAAEPIVRAQPHRVSIAVATVVVFGTLGMFLYPVLY
                     PHLGLSPQTYGIYAGSTIHEVAQVVAAGKAVSDSAAAAAVIEKMLRVMLLAPFLLLLS
                     RSQRDAARPRVTIPWFALCFLGVCAINSLNCFSPAAVSVLNNVDTLLLATAMAALGLR
                     THVRAVTQAGLRPLMLAGTLFSFLILGGYVVNRTLMSLLA"
BASE COUNT          597 a         1055 c         1051 g          555 t
ORIGIN      
        1 gatcggactt caacgacgct tcaatggagc atgcacatgt tcaggacagc gtgggctctc
       61 acgttttgcg ctctcctcgc ggcgcgggtc tacgcgtggg accaggatgc aggcgctcta
      121 ccggaggggg cgggcaagca gatcgcagcc tccaagtgcc tcggttgtca cgatgcgggt
      181 cggctcgcag cgccgggcta cacacgcgca ggctggcaga gcgtcatcgg tcggatggtg
      241 aagatgggcg cgcaggttga gcccgcccag atcccggcgc tgtccgatta tctcgcgcgc
      301 agctttccgg agcggccaga gcctgcagcg cgactgatcc caggatcggt gcgggtctcc
      361 ttccaggaat gggcagtcgc caccccagga gccttcccgc atgatccgct cgcgacggcc
      421 gatggggcga tctggtacac cggccagcat gccagcgtgc tcggccggat cgatccggcc
      481 agcggcgcga tcaaggagta tcccacccgg acagcggatt cgggtccgca tgggctcacc
      541 gccgatgccg cgggcagcat ctggttcacg gcaaactcgg ccggctacat cggcaagctc
      601 gatccgggta cggggaagat caccgagtac cgcatgcccg atgcgcgcgc ccgagacccg
      661 catacgccca tcttcgatcg tcacggtatc ctctggttca cggttcaggg tgccgacatg
      721 gtcggacgtc tcgagcccag taccggcaac gtaaagctcg tgtcagtgcc catacctcac
      781 gcgctgcctt atggcatcgt gatcagctct accggggtgc cgttcttcgc cgagttcggc
      841 tcgaaccgac tcgcgagtat cgatccccac accctgacca tccatgaata cgtgctgccc
      901 aacagcggct cgcgcccccg ccgcatagca atcagcagcg atgatgtcct gtggtattcg
      961 gactacgcgc gcgggtgcct cggtcgtttc gatccgaaat cgggcgctgc gcgggagtgg
     1021 ccctcccccg gcggtccgga ctcgcgaccc tacgccatca cggcgctgca ggatgtcctg
     1081 tggtacagcg agtccggggt cagccccaac acgctcgtgc gattcgatcc gcgctcggag
     1141 aaattccaga cctgggccat cccctccgga ggcggtgtcg tgcgcaacat gatgccggcg
     1201 cacgatggga ctctggtatt ggccgagagt ggcgccggca agctcgcgcg agtcacgatc
     1261 gagtaatcag agcgccgcag ggacaggcgc gcggcagcgc cggcaccgcc tgactactct
     1321 gtactttgac gcgggggcgc cgcgtccggg ctgccttcag gcgcgccccg atgcagcacg
     1381 tccggaaggg gcgctccccg ctccgtgaag ccgagggaga ctgaattgag gcaatggcgc
     1441 tcgccggtcg gcggcggccc gtcggtaaag acatggccga ggtgactacc gcagcgcgcg
     1501 caaacttcct ccacccgaat catcccgtag ctcgtatccc gcaggcgctc gatgtgcgcc
     1561 tcgtcataag gccggaagaa actgggccac ccggtaccgg aatcgaactt tgcactggag
     1621 cggaacaagg gcaatccgca gaagcggcag gtatagatcc catccttctt gttatccaga
     1681 aagacgccgc agaaggcggc ttccgtgccg tgttccagaa ggatgcgccg ttcattgcga
     1741 tcaaggccgt cggtaatctc gcgccgctgt gtgtcgctgg ggggagtcag atcgaagttg
     1801 ctcatgagag gccccgggca aagctccaag gtaatcgttc caggcgataa tacccccacc
     1861 ctatctggga gccgactgaa gtgttcaccc gagccattgt ccgacccccg ggcgcgagct
     1921 atgccgcggg cctcaccagc tccaccgaag gcgtgcccga tctgggccgt gcgattgcgc
     1981 agcatcacga gtactgcaat gccttgcgcg agtgcggcct cagtgtccat gccctgcagc
     2041 ccgatgagcg atatcccgac ggtactttcg tcgaggatac ggcggtcatt tccggcgcca
     2101 tggcggtggt cacccgaccc ggcgcgccga gccgcgaggg cgagatccag gggctcgctg
     2161 cacttcttca gggcttcggc ctgtcggtgg aggagatcac ggcccctggc agtgtggacg
     2221 gcggggatat ctgccagatc gagaaacact tcctgattgg attgtcccat cgcaccaacg
     2281 ctgaaggcgc tcggcagctt gcgcggattc tcgagcgata cggatacagt tcctccgaga
     2341 tcgacatccg caacaacaaa gccctgttgc atctgaagtc cggtctctcc tatctcggag
     2401 agaatcgcct cctcgtcgtg ccggatctga tcgttccgga gcagctgagc cgctatcagc
     2461 tcatcagcgt atccgctgaa gagggctatg ccgccaactg cgttcgcatc aatgaccgcg
     2521 tgctggtggc ggcggggtac ccgcgcctca gcgccagcct ggagcggctc ggctactcgg
     2581 cgctggcgct gccgatgtcg gaattccgca agatggatgg cggtctgagt tgcctgtcgc
     2641 tgcgattctg agtgctcagg cgagcaggct catgagagtg cggttcacca catagccccc
     2701 caggatcagg aaagagaaca gcgtcccggc cagcatcagc ggccgcaggc ccgcttgagt
     2761 gaccgcccgc acgtgggtgc gcaggccgag ggctgccatc gccgtggcca acagcagcgt
     2821 gtccacattg ttcagcacgc tgaccgctgc cgggctgaag cagttcagcg agttgatggc
     2881 gcacaccccg aggaagcaca gggcgaacca gggaatcgtc acgcgaggac gtgcggcatc
     2941 gcgctgcgag cgggagagca gcaacaaaaa cggagccagc agcatcacgc gcagcatctt
     3001 ctcgatgacc gcggccgcag cggctgaatc actcacggcc ttgccggctg ccaccacctg
     3061 tgcaacttcg tggatcgtcg agccggcata gattccgtag gtttgcgggg ataggcccag
     3121 atgtggatac aacacggggt acagaaacat ccccagggtc ccgaaaacca ccacggtagc
     3181 cacggcgatc gagactctgt ggggctgtgc gcggacgatc ggctcggcgg cgagtacggc
     3241 ggctgccccg caaatggc
//
LOCUS       DASRHN010000027         9548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1657142, whole genome shotgun sequence.
ACCESSION   DASRHN010000027 DASRHN010000000
VERSION     DASRHN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657142"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFB37_00890"
     CDS             <1..896
                     /locus_tag="VFB37_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21025.1"
                     /translation="IPWGGSQRLVVHEQIGGARVLDAMGRSDRALEWSGLFSGPDASQ
                     RAQFLDSLRVSGAQQSLTWAQFSYLVVVREFTATYQRFYRIPYHIVCEVVLDQSKPVT
                     SAPPTAVDDALLDDQAAADALAGAIGDGLLSSLMSGLDTAIAAVSTFANAAQSTINSV
                     LQPLAAVQARVSTLIGISSNALANVTTFGGVLPGTPPALVAGKLTDQTVNMVQATNLF
                     QMRNLLGRMDANLIAINVPQKTVATAGGNLFRIAQNQYGDAIAWTALARANGLTDPFV
                     QGSALLAVPPSPDKLGGVLNA"
     gene            906..2069
                     /locus_tag="VFB37_00895"
     CDS             906..2069
                     /locus_tag="VFB37_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21026.1"
                     /translation="MSVPADQIATVTVSATAQRVRAPRALVKLGGFGAGDIVPGWISW
                     SVSSNTFYEADSFRIEFATSALPSSNDANWFSTQRELFAEIFAGFPSDPQNPQLSELQ
                     SLIYGRVDEIEYDPAQRLITLTGRDLTAVFIDNKIASQYTNQTSSDIALTLAALHNLN
                     ATVAPTQTKVGTYYEQDQVQLQANRSEWDLLAYLARREGFVVYVQGTTLFFQPDPTNT
                     NEPYEIGWIAPDVATQAPISDALEIKFSRALTVAKGVAVTARSANFDAGRPIVQSYPS
                     SAKTIQAGKASPFGEVQNYFFTMAPGHSALEVEAFAKAQYDAIVAHEMKLSARLIADN
                     VLTTQIPIRIRGTGTAFDQIYYARKIEREMSLDEGYVMSVEAQNVNPDSSPQS"
     gene            2066..2671
                     /locus_tag="VFB37_00900"
     CDS             2066..2671
                     /locus_tag="VFB37_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HZO21027.1"
                     /translation="MIGPLQNAMRLQAQRALAYLVTTEIGTVTSYDPNKFSAKVLLQP
                     DELLTGWLPVASPWIGNGWGLFAPPNIGDLVSVEFINGNWGAGTVVGRFWNNANRPLP
                     VPSGEFWLVHSTGSYFKMLTSGEVTFSDARGATVSLDGHGNITSQAKQWTHTGPVHFT
                     DDVQVDGTLTANVDVIAAAISLLSHLHSGVSSGGSNTGPPV"
     gene            2693..3820
                     /locus_tag="VFB37_00905"
     CDS             2693..3820
                     /locus_tag="VFB37_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HZO21028.1"
                     /translation="MAAPQTQTFTSLLENAVAAVQGASRQLLDFTVGSVLRAVIESTC
                     ALALWLQAEALQIASLTRFSTSSGADADSWAADFAFERLAAQSATGPVLFARFTSTQQ
                     ATLNVGQIVQTADGTQKFAVIADTSQPSFDSTQNAYILQAGISEVLATVSAVVPGSAG
                     NVQVGFINTLGSAIPGVDTVTNLVAFTNGADAEPDAAFKARFVLYIQSLSKATPAAIS
                     AAIESVQQDVTNSLTENQMFDGTADPGNFYAVVDDGSGSPSASFLSGIANAIDLVRPI
                     GSTFQVFGPTLIQASVQMSLTIAAGYNQAQVDGLVQSAVEDYIASLSIGEPLPFTRLA
                     QLAYDASPGVTNVTNVLLNGGTADLVASNKQVIRPGVLTII"
     gene            3833..4498
                     /locus_tag="VFB37_00910"
     CDS             3833..4498
                     /locus_tag="VFB37_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21029.1"
                     /translation="MASGDQADILGRLQQLVPQGWFPVGAAPLRDGLLTGVANTLSFI
                     YGLLGYLRLQTRIATATDGFLDLIANDFFGAKIFRQANQTDDSFRAQIIANILRPRGT
                     RPAVSDLLTQLTGRTPIIFEPRRPADTGAYGNGSLAYNTAGGYGSLQYPYQSFVTVFR
                     PLGQGIPAVAGYGIPVGAYSTASQIEYVTSAFINGISDTDLIAAIESVRPVGYTIWVR
                     ISS"
     gene            4570..5565
                     /locus_tag="VFB37_00915"
     CDS             4570..5565
                     /locus_tag="VFB37_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21030.1"
                     /translation="MDRVIVYPGSIPQDVDVLSTNKNSLVALGMLMLDVLGSGTLASG
                     LKCVPTSPGSLNVSIQPGRLYSLQNIDNTAYGSLALDSTHQILKQGILLDALQLACPA
                     PSTSGFSINYLIEAAYQDSDTSATALPYYNSQNPQQPFSGPNNTGVAQPTVRQGSIVL
                     QAKPGSVAPTGTQNTPTPDVGFTGLWVVSVANGQSAITTSNIAMASGAPFLTGSFAGA
                     YTGYSGSITSSINWWRLGPAVYLTLAPVTGTSNGSGFGISNLPPAICPPTTQLISVPN
                     AAFVNNGAALGGVADVSAQVSASGLSFQFNNSLTGWTASSSKGIGAEVTFSYVLA"
     gene            5588..5785
                     /locus_tag="VFB37_00920"
     CDS             5588..5785
                     /locus_tag="VFB37_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21031.1"
                     /translation="MIQQKAADASIWAAVLVWFMSHLTEINAVLQFFLLIGSLVGVFF
                     AIRYHKRNTPRNSPPGAPPSL"
     gene            5782..6246
                     /locus_tag="VFB37_00925"
     CDS             5782..6246
                     /locus_tag="VFB37_00925"
                     /inference="COORDINATES: protein motif:HMM:NF040141.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HZO21032.1"
                     /translation="MNLSLIRDTATASYCQGVLQAGALRLQTIELPWVPFDGGRGGHP
                     AESCVSAGTYDLVLHDTVKHPKSFALSNPDLDVFHEPADVPAERRLYARIAILLHVAN
                     SPGELLGCIGVGMARGPGYVSQSRLAFDRFNTAVPWVAGHTLTITDAAWAQP"
     gene            6549..6809
                     /locus_tag="VFB37_00930"
     CDS             6549..6809
                     /locus_tag="VFB37_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21033.1"
                     /translation="MTFWQFLTNLCHALTSHGTRVLGVAVGTITALTTSNVIPANHLK
                     YYLAAIAVLTYWRGQGNADKIADKVIEKTQGPLAARPPEIPK"
     gene            6806..7081
                     /locus_tag="VFB37_00935"
     CDS             6806..7081
                     /locus_tag="VFB37_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21034.1"
                     /translation="MSTPNEAVVAAAPILVNVINELQSFVNTVLTGDPAQIALRFDGA
                     AKVLIGQIELQLPVLATSEIGVVNSDINAKLASWKNSLNALSAPKAA"
     gene            7078..7257
                     /locus_tag="VFB37_00940"
     CDS             7078..7257
                     /locus_tag="VFB37_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21035.1"
                     /translation="MTALQVLELIAAIGNASSALVKLIEALHAQGIDLSKPMQPEHQA
                     LADAAIDHMKKAMGG"
     gene            7259..7465
                     /locus_tag="VFB37_00945"
     CDS             7259..7465
                     /locus_tag="VFB37_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21036.1"
                     /translation="MGYILILLTLSATTGEGRYTRIQSFDTEAQCEAHFPLTVQRPQQ
                     GEVRVYQCVTGPKPEPKKPPGDHV"
     gene            7664..8092
                     /locus_tag="VFB37_00950"
     CDS             7664..8092
                     /locus_tag="VFB37_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21037.1"
                     /translation="MTCIAVKDSVMAADSLISGVYNTPAVKIYRKPDYLIGFAGEVAQ
                     ALVFVDWYQDRQCRIPDIAHEQDWCALVLSRKSGIEFWDRSLRPVPILERQAAIGSGA
                     PFAMAAMDAGLSAAAAVKIACKRDASCRLPIVTARLKPTK"
     gene            8099..8392
                     /locus_tag="VFB37_00955"
     CDS             8099..8392
                     /locus_tag="VFB37_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21038.1"
                     /translation="MTKAAAPTKIGKSIEAFRAAHDKNYIVPQKIRAALIKLGDGWEY
                     ELDFMRIAGVSSTDLATFREQFIDHIVTTSGKNPKRAWAGKKKLAEKLRAMSP"
     gene            8389..9456
                     /locus_tag="VFB37_00960"
     CDS             8389..9456
                     /locus_tag="VFB37_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21039.1"
                     /translation="MKTIEDFKQAHDPRTIIARLENELKRANEQSADSVAIKAAIGML
                     DAKLSTLNPPAWIEREVAKASSPGVPTLFLSDLHWGEVVHPGQINGVNKYNLSIARER
                     LRYTVNTAIHLLRILDRNMDYPGIVVPLGGDAISGNIHDELQATNELNTMPTVLDLYD
                     HLVGAIKLLAGTFGNVFLPCVTGNHGRDTKKIWAKDRHHTSFDWLLYQFLAKHFLSDK
                     RVRFYIPDGSDAFYRIFGTKYLLTHGDQFKAGDSIIGPLGPLTRGNQKKLARNTAVDM
                     AYDLMICGHWHQYIHLSRLIVNGSMKGYDEYAFQGNFGFELPMQALWVTHPRYGITYR
                     MPVYCEQPKGVPKAAWASVAA"
BASE COUNT         1750 a         3174 c         2875 g         1749 t
ORIGIN      
        1 agattccctg gggcggcagc cagcgcctgg tggtgcacga gcagatcggc ggggcgcggg
       61 tgctcgatgc catggggcgc tcggaccggg cgctcgagtg gagcgggctc tttagcggcc
      121 ccgatgcttc ccagcgcgca cagtttctcg attcgctccg ggtgagcggg gcgcagcagt
      181 ctctcacctg ggcacagttc agctatctgg tggtcgtgcg cgagttcacg gcgacctatc
      241 agcgcttcta tcggatcccg taccacatcg tgtgtgaggt ggtgctcgat cagagcaagc
      301 ccgtgacgag cgcgccgccc accgcagtgg acgatgcgct cctggatgat caggcggcgg
      361 cggatgcgct cgcaggggcc attggtgacg gcctgctcag cagcctgatg tctggcctgg
      421 atacggccat tgctgccgta tccaccttcg ccaacgcagc gcagagcacg atcaacagcg
      481 tcctgcagcc cctcgcggcg gtccaggcgc gcgtctcgac cctgattggg atctcgagca
      541 acgccctggc aaacgtcacg accttcggcg gcgtgctgcc tgggaccccg cccgccttgg
      601 tggccggcaa gctcaccgat cagaccgtga acatggtgca ggcgacgaat ctcttccaga
      661 tgcgcaacct gttggggcgc atggatgcga acctgatcgc catcaacgtg ccgcagaaga
      721 ccgtagcgac tgcgggcggc aatctgtttc gcattgcgca gaatcagtac ggcgatgcca
      781 tcgcctggac cgcgctcgcg cgcgccaacg ggctcacaga ccccttcgtg caaggctccg
      841 cactgttggc cgtgccgccg agcccggaca agctgggcgg cgtgctgaac gcgtaaggcg
      901 cgcccatgag cgtgccagcg gatcagattg cgaccgtgac ggtcagtgcg acggcgcagc
      961 gcgtgcgggc gccgcgggcg ctcgtcaagc tcgggggctt cggcgcgggc gatatcgttc
     1021 cggggtggat ttcctggtcg gtctcaagca ataccttcta cgaagccgat agctttcgga
     1081 ttgagtttgc gacctcggcg ctgccttcga gcaacgacgc gaactggttc tccacgcagc
     1141 gcgagctctt cgcggagatc ttcgcgggct tcccctcgga cccgcagaat ccgcagctct
     1201 ccgagttgca gagcctgatc tacgggcggg tcgatgagat cgagtacgac ccggcgcagc
     1261 ggctgattac cctcacgggc cgcgatctga cggctgtctt tatcgataac aagatcgcca
     1321 gccaatatac gaaccagacc tcgagcgaca ttgcgctcac gctcgcagcg ctgcacaacc
     1381 tgaacgccac ggtggccccg acgcagacaa aagtcggcac ctattacgag caggaccagg
     1441 tgcagctgca ggccaatcgc agtgagtggg atctgctggc ctatctggca cggcgcgagg
     1501 gcttcgtggt gtatgtgcag gggacgacgc tcttttttca acccgatccg acgaacacca
     1561 acgagccgta tgagatcggc tggattgcgc ccgatgtggc cacgcaggcg ccgatctccg
     1621 atgcgctgga gatcaagttc agccgcgcgc tgaccgtcgc caaaggcgtc gccgtgaccg
     1681 cgcgcagtgc gaatttcgat gccgggcgcc cgattgtgca gtcctatccc tcctccgcca
     1741 agacgatcca ggccggtaag gcgtcgccct tcggcgaggt gcagaactat ttcttcacca
     1801 tggccccggg gcactcggcg ctcgaggtcg aagccttcgc gaaggcccag tacgatgcca
     1861 tcgtggcgca cgagatgaag ctctcggcgc ggctgatcgc ggataacgtc ctcaccacgc
     1921 agatcccgat ccgcatccgg ggcacgggca ccgcattcga tcagatctac tacgcccgca
     1981 agatcgagcg cgaaatgagc ctggatgagg gctacgtgat gagcgtggaa gcgcagaacg
     2041 tcaaccccga cagttcgccg cagtcgtgat cggtccgttg cagaacgcca tgcgcttgca
     2101 ggcgcagcgt gcgctcgcct atctggtgac gaccgagatc ggtacggtca ccagttacga
     2161 cccgaacaaa ttttcggcca aagtgctgct gcagcccgat gagttgctga cgggctggct
     2221 accggtggcc tctccctgga tcggcaacgg ctggggactc ttcgctccgc cgaacatcgg
     2281 cgatctcgtc tcggtggaat tcatcaatgg caactggggc gccggtaccg tggtggggcg
     2341 gttctggaac aacgccaacc ggccgctccc ggtgccctcg ggcgagttct ggctcgtgca
     2401 ctctacgggg tcgtatttca agatgttgac ctcgggcgaa gtgacttttt ccgatgcccg
     2461 cggcgcgacc gtgagtttgg atggccacgg caacattacc tcgcaggcga aacagtggac
     2521 gcataccggt cccgtgcact tcaccgacga tgtgcaggtg gacggcacgc tgactgccaa
     2581 cgtcgatgtg atcgcggccg ccatcagtct cttgagccac ttgcacagcg gtgtctcgag
     2641 cggcggtagc aatacgggac ccccggtcta accaggaatc acgaaccgag ccatggccgc
     2701 ccctcagact cagaccttca cgagccttct ggagaacgcc gtggcggccg tccagggcgc
     2761 ctcgcggcaa ctcctggact tcaccgtggg gtcggtcctg cgggccgtca tcgagtcgac
     2821 ctgcgccctt gcgctgtggt tgcaggccga ggccctgcag attgcctcgc tcacccgctt
     2881 ctcgaccagc agcggcgcgg atgcggacag ttgggcggct gatttcgcct tcgagcggct
     2941 tgctgcgcaa tcggccaccg gccccgtcct cttcgcgcgc ttcacctcga cgcagcaggc
     3001 gacgctcaac gtcgggcaga tcgtacaaac cgccgatggc acgcagaagt ttgcggtcat
     3061 cgccgacacg agccagccat ccttcgactc gacccagaac gcctacattc tgcaggcggg
     3121 gatctcggag gttctcgcca cggtctcggc ggtcgtgccc ggcagtgccg gcaatgtcca
     3181 ggtcggcttc atcaatacac tcggcagcgc gataccgggg gtcgataccg tcaccaacct
     3241 cgtcgccttc accaacggag cggatgcgga accggacgcc gccttcaagg cgcgcttcgt
     3301 gctctacatt cagagcttgt cgaaggcgac ccctgcggcg atcagcgcgg cgatcgaatc
     3361 ggtgcagcag gacgtcacga acagcctgac cgaaaaccag atgttcgacg ggaccgccga
     3421 tcccggcaat ttctacgcgg tcgtcgatga tggctcgggc agcccttccg ccagcttcct
     3481 ctcaggcatc gctaacgcca tcgacttggt gcgcccgatc ggctcgacgt ttcaagtctt
     3541 cggcccgacg ctgatccagg cgagcgtgca gatgagtctg accattgccg ccggctacaa
     3601 ccaggcgcag gtcgatggcc tggtgcagag cgcggtggag gactacatcg cctccttgag
     3661 tatcggtgag ccgctgccgt tcacgcgcct cgcacagctt gcctacgacg cctccccggg
     3721 cgtgacgaac gtgaccaacg tgctgctcaa tggcggtacc gcagacctcg ttgcctccaa
     3781 taagcaggtc attcggcctg gcgtcctgac aatcatctga aggcgcgcag ccatggccag
     3841 cggtgatcag gcggacattc tcggccgctt gcagcaactc gtgccgcagg gctggttccc
     3901 ggtgggcgcc gccccgctgc gcgacgggct gctcacgggc gtcgccaata cgctcagctt
     3961 tatctacggc ctgctgggct acttgcgtct gcagactcgg atcgcgaccg cgaccgatgg
     4021 ctttctcgat ctgatcgcca acgacttttt cggcgccaag atctttcgcc aagcgaatca
     4081 gaccgatgac agcttccgcg cgcagatcat cgccaatatc ttgaggccgc gcggcacccg
     4141 ccccgccgtg tcggacctgc tcacgcagct caccggccgc acgccgatca tctttgaacc
     4201 gcggcgcccg gcggatactg gtgcgtacgg gaacggctcg ctcgcctaca acaccgcagg
     4261 cggctacggc tcgctgcagt acccgtatca gtctttcgtg accgtctttc gcccgctggg
     4321 ccagggcatc ccggccgtcg ccggctatgg gatcccggtg ggcgcgtatt cgacggcctc
     4381 gcagatcgag tacgtcacgt cagcatttat caacggcatc agtgataccg acctgattgc
     4441 cgccatcgag agtgtgcgcc cggtgggcta caccatctgg gtgcgtatct cgagctgatt
     4501 tttttcactg ttaccgaacc gccaagacgg gccgctcgct gcggcccgtt ttcgttggag
     4561 gctgcccctt tggatagagt tatcgtctac cccggttcga ttccccagga cgtcgatgtc
     4621 ctgagcacga acaaaaactc cctcgtcgct ctcggcatgc tgatgctcga tgtgctcggc
     4681 tcgggcaccc tggccagcgg cttgaaatgc gtgcccacca gtccgggctc gctgaatgtg
     4741 tccattcagc ccgggcgcct ttactcactg cagaacatcg acaacaccgc gtacggctcc
     4801 ctcgcgctcg actctacgca ccagatcctg aagcaaggca tcctgctcga tgcgctacaa
     4861 ctcgcctgtc ctgcgcccag cacctcgggc ttctcgatca actacctgat cgaagcggcg
     4921 taccaggaca gcgataccag tgccaccgcg ctgccgtact acaactcgca gaatccgcag
     4981 cagccgttct ccggtcccaa caataccggt gtcgcccagc ccacggtccg ccagggctca
     5041 atcgtgctgc aggcgaaacc gggctccgtt gcgcccacgg ggacgcagaa cacgcccacg
     5101 ccggatgtcg gcttcacagg cttgtgggtg gtttcggtgg ccaacggcca atccgccatc
     5161 accacgagca acatcgcgat ggcctcgggc gccccgttcc tgacgggctc gttcgcgggg
     5221 gcctataccg gctattcggg ctcgatcacc tcgagcatca actggtggcg gctggggccg
     5281 gcggtgtatc tcaccctcgc gccggtcaca ggcacgagca acggcagcgg tttcggtatt
     5341 tctaatttgc cgcccgcgat ttgcccgccg accacccagc tgatctcggt ccccaatgcg
     5401 gcgttcgtca ataacggcgc agcgctcggc ggggtcgccg atgtgtctgc ccaagtgagc
     5461 gctagcggac tgagctttca attcaacaat agcctcaccg gctggaccgc ctcgagcagc
     5521 aagggcatcg gagccgaggt gaccttctcg tacgtgctag cttgatcggc taaagggaac
     5581 gcagccaatg atccaacaaa aagccgcaga cgcctcgatc tgggccgcgg tcctggtgtg
     5641 gttcatgtca catctcactg aaatcaacgc cgtcctgcag ttcttcctgc tgatcggctc
     5701 gctcgtcggc gtcttcttcg cgatccgtta ccacaagcgc aacacgcctc ggaactcgcc
     5761 cccgggtgcg cctccgagcc tatgaacctc tcgctgatcc gcgataccgc gacggcttcc
     5821 tactgccagg gcgtgctgca ggcgggcgcc cttcggttgc agaccatcga attgccttgg
     5881 gtcccgttcg atggtggccg cggcggtcat cctgcggagt cgtgtgtgtc cgccgggacc
     5941 tatgacctcg tgctgcacga cacggtgaaa catccgaagt cgtttgcact gtcgaatccg
     6001 gatctggacg tcttccacga gccggcagat gttcccgccg agcgccggct ctacgcgcgc
     6061 atcgccatcc tcctgcatgt ggctaatagc cccggggaac ttttagggtg catcggcgtc
     6121 ggcatggcgc gcgggcccgg gtacgtgtcg cagtcgcgct tggccttcga tcgcttcaac
     6181 actgcggtcc cgtgggtcgc cgggcacacg ctcaccatca ctgacgcggc gtgggcccaa
     6241 ccctgacgta ccgtgtccgc tcggacctac atgggccgcc cgtgccggaa ggggcacgac
     6301 ggcgagcgta agaccgagcg ctacgtctct tcgggctact gcgtgaaatg ctcgctcgag
     6361 cgcggaaggc gctatcgaga gggccgagcg aagaaacctc cctgcgaacg gccgctcgct
     6421 gagcgtttca ccgcgattcg tccctgtctt cagtgccggg atcccttcat cgcaagggac
     6481 ccggccgccc agtattgcgg gattgcgtgc ttcctgcgtc accgcccgat cccgcagagg
     6541 aaattcgcat gacgttttgg caattcctaa ccaacctctg ccatgcactc acgagtcatg
     6601 gcacccgcgt gttgggtgtg gccgtcggca cgatcaccgc gctcaccaca tcgaatgtga
     6661 tcccagccaa ccatttgaag tattacctcg cggcgatcgc cgtcctcacc tactggagag
     6721 ggcagggcaa cgccgacaag atcgccgaca aggtgatcga gaaaacccaa ggccctttgg
     6781 ccgcacgtcc accggagatc ccgaaatgag caccccgaac gaagccgtcg tcgccgctgc
     6841 ccccatcctc gtgaatgtca tcaacgagct gcagtccttt gtcaacaccg tcctgacggg
     6901 tgatccggcc cagattgcct tgcgcttcga tggcgcggcc aaggtcctca tcggccagat
     6961 cgagctgcag ctgccggtgc tcgccacgtc tgaaatcggc gtggtaaaca gcgacatcaa
     7021 cgcgaagctg gccagctgga aaaacagcct aaatgccctg tctgcgccga aagccgcatg
     7081 acagcgctgc aggtactgga gctgatcgcg gccatcggca atgcgagctc ggcgctggtg
     7141 aaactgatcg aggccctgca tgcgcagggc atcgatttgt cgaagcccat gcagcctgag
     7201 catcaggcgc tcgccgatgc cgcgatcgat cacatgaaga aggccatggg cggctgatat
     7261 gggctacatc ctcatcctgc tcaccctctc ggcgaccacg ggggaggggc gctacacccg
     7321 catccagagc ttcgacaccg aagcgcagtg cgaggcgcac ttcccgctca cggtccaaag
     7381 gccgcaacag ggcgaagtgc gcgtgtatca gtgcgtgact ggcccgaagc cggagccgaa
     7441 aaagccgccg ggagatcacg tgtgaggcgg tgggtgccga ccctctgcct cctcatgctc
     7501 gtaattccgt attccgacgg caccccgctt ccgccgctca ccggcccgct attcgatcag
     7561 ttcaaccgcc tcaccgcgcg gccaaatccg tggccgcccg tgtcgatctt cctgagcgtg
     7621 atcgctgcgc tcgccgttcc gatcaacagg aaggcagatc cacatgacct gcatcgccgt
     7681 taaagattcg gtcatggcgg ccgattccct catttccggt gtctacaaca cacccgccgt
     7741 caaaatctac cggaagccgg actacctcat cggctttgcg ggcgaagtgg cgcaagccct
     7801 cgtcttcgtg gactggtatc aggaccggca gtgccgcata cccgatatcg cccatgagca
     7861 ggattggtgc gccctcgtgc tctcgcgtaa gtctggcatc gaattctggg atcgatcact
     7921 gcggcccgta ccgatcctcg agcgtcaggc cgccattggt agcggcgcgc ccttcgcgat
     7981 ggcggccatg gatgcggggc tcagtgccgc cgcggccgtc aagatcgcct gcaagcgcga
     8041 tgcgtcctgc cgccttccca ttgtcacggc tcgactcaaa cccaccaagt gaggcgtcat
     8101 gaccaaggcc gcagctccca cgaagatcgg taagagcatt gaggcgttcc gcgccgccca
     8161 cgataagaac tacatcgtcc cgcagaaaat ccgcgcagcg cttataaagc tcggcgacgg
     8221 gtgggaatac gaactcgact tcatgcggat cgcgggtgtg agttccacgg atctcgccac
     8281 attccgtgag cagttcatcg accacatcgt gacgaccagc ggaaagaacc cgaagcgcgc
     8341 ctgggcaggt aaaaagaagc tagccgaaaa actacgggct atgtccccat gaagacgatc
     8401 gaggacttca agcaagcgca cgacccgcgc acgatcatcg cgcggctcga gaacgagcta
     8461 aagcgggcga acgagcagag cgccgactcg gtagccatca aggctgcgat cgggatgctc
     8521 gatgccaagc tctcgacgct caacccgccc gcatggatcg agcgcgaagt cgccaaagcc
     8581 tcctcgccgg gcgtgccgac gctctttctg tccgacctgc actggggcga agtggtgcac
     8641 cccgggcaga tcaacggtgt caacaagtac aacctctcga tcgcccgcga gcgcctgcgc
     8701 tacaccgtaa acaccgccat tcacctgctt cgcatcctcg atcggaatat ggactacccg
     8761 ggcatcgtcg tcccgctcgg aggagatgca atcagcggca acattcacga cgaattgcag
     8821 gcgacgaacg aactcaacac gatgcctacg gtgctcgacc tgtacgacca tctcgtgggc
     8881 gcgatcaagc tgctggcggg taccttcggc aacgtctttc tgccctgcgt cacaggcaat
     8941 cacggccgcg atacgaagaa gatctgggcg aaggaccggc atcacacctc cttcgactgg
     9001 ctgctgtatc agttcctcgc caagcacttc ctgtctgaca agcgcgtgcg gttctacatc
     9061 ccggacggct ccgatgcgtt ctatcgcatc ttcggcacga agtatctgct gacccacggc
     9121 gatcagttca aggccgggga ttcgatcatc gggccgctgg gcccgctcac gcgcggcaac
     9181 cagaagaagc ttgcgcgcaa taccgccgtc gatatggcct acgaccttat gatctgcggc
     9241 cactggcatc agtacatcca cctgtcgcgg ctgatcgtca acggctccat gaagggatac
     9301 gacgagtacg ccttccaggg gaatttcggc ttcgagctgc cgatgcaggc gctctgggtc
     9361 acgcacccgc gctacgggat cacttaccgc atgccggtgt actgcgagca accgaagggc
     9421 gtcccgaagg ccgcttgggc gagtgtcgcg gcgtgagcca gacgaagatc ggctctttcg
     9481 tggaagcctg ggccaacatc gcggtcggct ttgcgatcaa ctggaccgcc aacatgctgc
     9541 tgctgcct
//
LOCUS       DASRHN010000028        14114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_9757, whole genome shotgun sequence.
ACCESSION   DASRHN010000028 DASRHN010000000
VERSION     DASRHN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14114
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9757"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFB37_00965"
     CDS             <1..282
                     /locus_tag="VFB37_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZO21040.1"
                     /translation="AVRPEKIQISREPPPPSAAGPAHNGSAAANLVRGAVQEIAYMGD
                     VSIYLIRTETGKMVRVTLPNVARHAEDRITWDETVYLSWHAASPVVLTR"
     gene            327..1193
                     /locus_tag="VFB37_00970"
     CDS             327..1193
                     /locus_tag="VFB37_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO21041.1"
                     /translation="MVIGIPSLWLIVLFLIPFIIVFKISFSEVRLAMPPYAPLFEWLN
                     GRPAAIRLNLANYAYLFTDALYISSYLYSLKVAAVSTLFCLLLGYPMAYSIARAGTTS
                     RNVLLMLIVLPFWTSFLLRVYAWMGLLKSNGLINNALLWLGVIHEPITMLQTDFAVYI
                     GIVYSYLPFMILPLYANLEKHDPTLLEAAADLGARPLTVFRRITLPQSLPGIVAGSLL
                     VFIPAVGEYVIPSLLGRTDQLMIGRVLSDEFFENRDWPVASAVAIIMLALLVIPIMVF
                     QHFEKRSLEAAK"
     gene            1190..2038
                     /locus_tag="VFB37_00975"
     CDS             1190..2038
                     /locus_tag="VFB37_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO21042.1"
                     /translation="MKSRGMFSRTALVLGLVFLYVPILSMVVFSFNNSRLVTVWDGAH
                     SPTLKWYIELASNDQILDAAWLSIKIAITAACVSVVLGTLSGVALARFGAFRGRALLA
                     GMTTAPLVMPEVITGLSLLLLFVALEDAIGWPRGRSAVTITLAHVTFCMAYVTVVVQS
                     RLVGFDESLEEAAMDLGARPAKVFFRITLPLIVPALMSGWLLAFTLSWDDVVISQFVA
                     GPSSSTLPMVIFSKVRLGVSPDVNALATLMVLVVACGIVAASIWMSYQNRRRERDMQL
                     AAAENL"
     gene            2049..2792
                     /locus_tag="VFB37_00980"
     CDS             2049..2792
                     /locus_tag="VFB37_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO21043.1"
                     /translation="MTRQVLGFDVGGSSVKAGVVDVEAGRLIGELISAPTPQPSTPAA
                     LMPVLSGLVLRLPAAEAAIGVAFPSVVKQGKVRTAANIDASWIGADGASLAAHALGRP
                     ALFLNDADAAGVAEVTWGAGRGVMGTVIMLTLGTGIGTALFVDGRLLPNTELGHMELH
                     GMDAEKWASAHVRTILKLDWPAWIERVNDYLASMHALFWPDLFILGGAVSERFAEFAP
                     LLRSEAQIRPAHFAGQAGVIGAAMAAAQQ"
     gene            2818..3984
                     /locus_tag="VFB37_00985"
     CDS             2818..3984
                     /locus_tag="VFB37_00985"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HZO21044.1"
                     /translation="MRSKLPDVGTTIFTVISRRARELNALNLGQGFPDYDIDPRLTEL
                     VSAAMRSGHNQYAPMEGLIELREEIARKLSQTYGLQIDPESQITVTLGATEAIYCAIQ
                     AVIGPGDEAIAFDPAYDSYEPAVRLAGGRCIRLPLTAPQFGYDWDAVRDAIGPRTRLI
                     LFNSPQNPACTAACAEDLTALAEVIRDREIFVMSDEVYEHVTYDGRSHHTILRQPELA
                     SKSYVIFSFGKTLHATGVRVGYCVAPPALTTELRKVHQFNTFSISSALQFAIAGYLRE
                     KPESWRTLAGFFQAKRDRLRGALAASGFHLPPAQGTYFQLLDYREFSQVADLAFAERL
                     LSEAGVATIPLSPFYANPPALTLLRLCIAKKDSTLDEAAQRLNAFAAALGKAGS"
     gene            4011..5180
                     /locus_tag="VFB37_00990"
     CDS             4011..5180
                     /locus_tag="VFB37_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO21045.1"
                     /translation="MKKTTRVNHPPEVELPPDNRALVAPIYQSVKFEFDSLGETERFL
                     RGERSGFFYSRASNPTTRQLELLLAELQGREDCLVTASGVGAIAQTLLALTRQGDHIV
                     CFIETYNPTRYLIRRLLARFGVTHTMLSIEDLAGVEQSLAAKRTRLVIFESPTNPVTK
                     IADLTALTKMARSAGALTVLDNTFAGFHQHGEYEVDLFLHSLTKYASGAGDVMGGAAI
                     GRADLIHSMRTDFGILGGTLDPHAAFLILRGLKTYFVRYQAQCASAMRIAEVLAAHPA
                     VSRVHYPGLPTHAQHELARSQMREFGSIVTLDLKGGAEAGGQFAGALKLFAMAASLGS
                     TESLVVAPQMMGSRDLSPEQLRLSGLAEGTVRLSIGLEDSDDLIEDVRQALASAG"
     gene            complement(5182..6936)
                     /gene="aceK"
                     /locus_tag="VFB37_00995"
     CDS             complement(5182..6936)
                     /gene="aceK"
                     /locus_tag="VFB37_00995"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZO21046.1"
                     /translation="MAITESPAQPSTSSLAHECAQQIAAAFVRYNAEFRSITRRAPDR
                     FDARDWKGSQRDAVERIELYDHFVGQTITQLCERLGAQAHDRELWSRIRTDFAAMIEG
                     LADEEFTKTFFSSITRRLFGTVGVAPGIEFVATDLDPLGKVTRTVGTNTYLNHGSLHL
                     LFEDVLGDVRFHSAWRDFDRSVEHVATETKRHLKDRNERRAVLRVEIIRPVFYQVSRA
                     YIVGRIVGREFLVPLVIALKNTDGGMLVDAVMLSEGDVSIVFSFTKSYFHVDLDRVGE
                     AVLFLKSMLPRKPVSELFTVLGRAKQGKTERYRELMRHLEQTQVQFVHAPGERGLVMV
                     CFTLPSFDVVFKVIRDRFAYPKTVVREEVMAKYQLVFKHDRAGRLVDAQEFKRLRFPR
                     ALFAPELLQELRAETAATVHEEGDDLIFDHMYIERRMTPLNLYLRTATAAAAERAVLD
                     YGQCIRDLAYSNIFAGDLLLKNFGVTRHGRVIFYDYDELCQVTDCHFRDVPQPANPED
                     EMRGEAWFYVADNDVFPETFISFLGFNDAQRAAFMRLHGEILTATFWRNVQQRLREGE
                     VLEVLPYHPHRVPVAGSA"
     gene            complement(6943..7584)
                     /locus_tag="VFB37_01000"
     CDS             complement(6943..7584)
                     /locus_tag="VFB37_01000"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZO21047.1"
                     /translation="MTPASDAGGNILQWARRVQAIAHNGLLFTKDPFDRERYTQLQEL
                     VASILAAELDIPVGQVRNLWSHDEGYATPKVDVRGGVFDGDEVLLVRERSDGKWTLPG
                     GWVDINDAPSGAVEREILEESGYRARAVKLAALLDKNNPAHGHPPGVHHIFKLFFLCE
                     RVGGSPATSTETDAVDYFPVKALPSLSLGRATPSQIELLYRHQLDRGLPTEFD"
     gene            complement(7581..8027)
                     /locus_tag="VFB37_01005"
     CDS             complement(7581..8027)
                     /locus_tag="VFB37_01005"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HZO21048.1"
                     /translation="MKTAGYSGTPLARKLGIKENSRLFLQDAPPNYLELVAPLPPGVK
                     LVSRIDATTDIVHLFVTFRASLSKICRHAMERLRPDGTIWVSWPKKASGVRTDVTEDA
                     IREIALPMGLVDVKVCAVNETWSGLKLVLRKENRHRAPARAAKGVS"
     gene            complement(8044..9351)
                     /gene="icd"
                     /locus_tag="VFB37_01010"
     CDS             complement(8044..9351)
                     /gene="icd"
                     /locus_tag="VFB37_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZO21049.1"
                     /translation="MSTAAASKITPPAQGARISIRDGKLLVPDNPVIPFIEGDGTGPD
                     IWRASVRVMDAAVQKAYGGKRKIHWMEVLAGEKANRQLNTWLPDETVAACREYLVSIK
                     GPLTTPVGGGIRSLNVALRQMLDLYVCLRPVRWFKGVPSPVKHPERVDMVIFRENTED
                     IYAGIEFETGSEENRRFLELFKQAFPKAYAKIRFPDSSGIGIKPVSREGSERLIRSAI
                     QYAVANRRKSLTLVHKGNIQKFTEGAFRNWGYQLAEREFAAQTYTWEQWERTRASGGE
                     KQANTEMDAAKQAGKVIVKDAIADIALQQVLTRSDEFDVIATLNLNGDYLSDALAAQV
                     GGIGIAPGGNINYLTGHAVFEATHGTAPKYANLDKVNPGSVILSGEMMLRYLGWLEAA
                     DAIISAMDRTIGQKIVTYDFARLMEGAKEVSTSEFGNTLIRNL"
     gene            9529..10974
                     /locus_tag="VFB37_01015"
     CDS             9529..10974
                     /locus_tag="VFB37_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO21050.1"
                     /translation="MASILRECFAVRPVTEADKEGGGRLKRVLTVWSLTAIGLGATIG
                     TGVFVLTGQVAANQAGPAVTLSLLIAAFGSGLAALCYAELAAFLPVPGSAYSYTYATL
                     GEAVAWFIGWNLLLEYGMSASAVAVSWSAYTVNVLESWHINLPKALTNAPLEAVNDHI
                     VATGAVFNVPAAFIIVAMSAICYVGMRETAGINTFMVALKVTIIVLFILAGLSYVDTA
                     NWHPYVPPATGEFGHFGWTGVMQGAAIIFFSYVGFDTASTTALEARNPQRDLPLGIIA
                     ALVISAVLYVATASVTTGMVSYTHLGVDAPVAVALDAHPQLAWLGRFVKIGAIVGMTS
                     VVLGSLLGQPRILLAMADDGLLPPAIARCHPRFKTPHIATVVTGVVFALIAGVFPLDI
                     LADLISIGILLAFVVVCIGVLIMRRTRPDAPRPFRVPWAPFTCVLGAVFCSAMACFLS
                     KGTWWRLVFWTILGFAIYAFYGFKHSRLRKR"
     gene            10971..12089
                     /gene="aguA"
                     /locus_tag="VFB37_01020"
     CDS             10971..12089
                     /gene="aguA"
                     /locus_tag="VFB37_01020"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HZO21051.1"
                     /translation="MTCTLTSTPAADGFWMPAEFEPHAGCWMLWPERPDNWREAARPA
                     QLAFVAVASAIAQFEPVTIGASASQYTTARALLPEHIRLVELAHDDSWMRDVGPTFLV
                     NARGAVRGVDWPFNAWGGLNGGVYFPWDQDDLVARKVLEIEARERYRAPLINEGGALH
                     VDGEGTALVTEQCLLNANRNPSLDRSQIETALRDYLGVSCVIWLSQGVCNDETDGHID
                     NLACFVRPGEVCLGWTDDRNDPQYALSLDAWERLQEARDARGRRLKVHRLPMPGPLRL
                     QAREAGGVVAREGTKPRLAGVRLAGSYINFYIANGGIIMPLLDRRTDRAAASRLKRLF
                     PKRRVVGVAAREILLGGGNIHCITQQVPCAGQKRKSPL"
     gene            12246..>14114
                     /locus_tag="VFB37_01025"
     CDS             12246..>14114
                     /locus_tag="VFB37_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZO21052.1"
                     /translation="MRTHPHAQAHAGIHRIIIKLRPASGSAAASSKLQMRAADRSAAL
                     ASRTGVTLKATRPITADLQALEVVPAVAEEGIEATLARLRADSEVEYAEADQRRYIHA
                     VPNDSLYATQQWYMQSSAATPSAVNAEGAWDTTNGSAGLVIADLDTGIRFDHPDLLWA
                     GQGSGGRLLPGYDFITDPFVANDGDGWDADPSDPGDWVTSADLKQTECSNITTVSNSS
                     WHGTRTAGMLGALTNNSAGIAGMTWNAWILPVRVLGKCGGLDSDIITGMLWAAGVHVA
                     GAPDNPYPARILNLSLGGTGACPTSYVDTITQLSQLGVLVVVSAGNEGGPVDAPANCP
                     GVAGVAGLRHAGTKVGFSSLGPELSLGAPAGNCVNTAPGSACIYSIGTTFNLGTTTPG
                     QNSYTDQLANPNLGTSFSAPIVSGIAGLMLAVNGNLGSAQLIARLKEGALPFPQTSLG
                     ETPQPPACHVPSGTSDVQDAECICTTDGRTCGAGMANAAGALAAALRPIAAVQAAATV
                     MAGQTVSMDASGSGAACGHNIASYQWVSSDPTHHPVSSSTGPTTSVVAPASGSVTISL
                     LVTDEAGRTDSAIIVVSPTSTTTSAPVSAGANACLKTIAVASPITLSVQPASATVPS"
BASE COUNT         2402 a         4482 c         4528 g         2702 t
ORIGIN      
        1 gccgtgcggc ccgagaagat ccagatcagc cgcgagcccc cgccaccctc cgccgccggc
       61 cccgctcaca atggcagcgc cgctgcgaac ctggtgcgtg gcgcggtgca ggagatcgcc
      121 tacatggggg atgtctcgat ctatctcatc cggaccgaaa ccggaaagat ggtgcgggtc
      181 acgctcccca acgtcgctcg ccacgccgag gaccggatca cgtgggatga gaccgtttac
      241 ctctcgtggc acgccgcaag tccggtcgtg ctgacgcgat aggcgggcgc atcttgggcc
      301 gactcggcgc aaaggcaggc aggcggatgg tgatcggcat tccgagcctg tggctgatcg
      361 tcctgttcct catccctttc atcatcgtat tcaagatctc cttctccgag gtgcgcctcg
      421 cgatgccacc ctacgcgcca cttttcgagt ggctcaacgg caggcctgcg gccatccggc
      481 tgaatctggc gaattatgcc tatctgttca cggatgccct gtacatcagc tcatacctgt
      541 attcgctgaa ggtcgcggcg gtttccacgc tgttctgcct gctacttggc tatccgatgg
      601 cgtactcgat cgcccgcgcc gggacgacct cgcgcaatgt gctgctgatg ctcatcgtgc
      661 tgccgttctg gacctcattc ctgctgcggg tctacgcatg gatggggctg ttgaaaagca
      721 atggcctcat caacaatgcg ctgctgtggc tcggagtcat tcacgagccc atcacgatgc
      781 tgcagaccga ctttgcggtg tatatcggta tcgtgtactc gtatctgccc ttcatgatcc
      841 tgcccctata cgccaatctc gagaagcacg accccacact gctggaagcg gcggcggatc
      901 tgggggcacg tcccctcacc gtgttccgcc ggatcaccct gcctcagtca cttccgggga
      961 tcgtcgccgg atcgctgctg gtgttcattc cggcggtcgg cgagtacgtc atcccctcgc
     1021 tgctcggtcg cacggatcag ctgatgatcg ggcgcgtgct gtcggatgag tttttcgaga
     1081 accgggactg gcccgtggcc agcgcggtgg cgatcatcat gctcgcgctg ctggtcatcc
     1141 cgatcatggt gttccagcat ttcgagaagc gcagcctgga ggcggccaag tgaaatcgcg
     1201 cggaatgttc tcgaggacgg cgctcgtgct ggggctcgtg ttcctgtatg tgccgatcct
     1261 gtcgatggtc gtcttctcct tcaacaattc ccggctcgtc acggtgtggg atggtgctca
     1321 ctcaccgacc ctgaagtggt acatcgagct cgcttccaac gatcagatcc tcgatgcggc
     1381 ctggttgtcg atcaagatcg cgatcaccgc ggcctgcgtg tcggtcgtgc tgggtacgct
     1441 gtcgggcgtc gcgctcgcgc ggtttggtgc cttccgtggc cgggcgctcc tggcgggcat
     1501 gacgacggca ccgctcgtca tgcccgaggt gatcacgggg ctgtccctgc tgctgctgtt
     1561 cgtggcgctg gaggatgcca tcggctggcc acgaggtcgc agtgccgtca cgatcacgct
     1621 cgcgcacgtg accttctgca tggcctacgt caccgtggtg gtacagtcgc gtctggtggg
     1681 ctttgacgag tccctggaag aggcagcgat ggatctgggc gcgcggcccg cgaaagtgtt
     1741 cttccgcatc accttgcccc tgatcgttcc tgcgctcatg tcgggctggc tgctcgcctt
     1801 cacgctctct tgggatgatg tggtcatctc gcagttcgtc gccggcccga gctcgagcac
     1861 gctgcccatg gtcatcttct ccaaggtccg gctgggcgtc agccccgacg tcaacgctct
     1921 ggcgaccctc atggtgctgg tcgtggcctg cggaatcgtg gccgcctcga tatggatgag
     1981 ttaccagaac agacgacggg agcgcgacat gcagcttgcc gcggccgaga acctatgagt
     2041 ccctcgtcat gacacgccag gtgctgggct tcgatgtggg aggctcctct gtcaaagcgg
     2101 gcgtcgttga cgtcgaggcg ggtcggctga tcggtgagct gatctccgct cccacgccgc
     2161 agccttccac tccggctgcg ctgatgccag tactgtccgg cctggtgctg cgcttgccgg
     2221 cggcagaggc agcaatcggc gtcgcctttc cgagtgtcgt caagcagggg aaggtgcgca
     2281 ccgcggcaaa cattgatgcg tcatggatcg gagccgatgg cgcctcgctc gcagcgcatg
     2341 ccttgggccg gcccgctctg tttctcaacg acgcggacgc cgcgggcgtc gccgaggtca
     2401 cctggggagc cggccgcgga gtcatgggca ccgtcatcat gttgacgctg ggcaccggaa
     2461 tcgggacggc attattcgtc gatgggcgcc tccttcccaa caccgagctc gggcacatgg
     2521 agctgcacgg tatggatgcc gaaaaatggg cctctgcaca cgtgcgcacg atcctcaagc
     2581 tcgactggcc agcctggatc gagcgggtca acgactacct ggcaagcatg cacgcgctct
     2641 tctggccgga tctgttcatc ctggggggag cggtcagcga gcggttcgcc gagtttgctc
     2701 cactgctgcg ctccgaggcg cagatccgtc cggcacattt cgccgggcag gcaggcgtga
     2761 tcggcgcggc gatggccgct gcgcagcaat aaggtccgtg cccggatgag ccctcccttg
     2821 cgcagcaagc ttcctgatgt cgggaccacc attttcacgg tgatctcgcg ccgcgcacgc
     2881 gagctcaatg cactcaacct cggacagggc ttccccgatt acgacatcga tccgcgcctg
     2941 acggagctgg tcagcgccgc catgcgctcg ggccacaacc agtacgcacc catggaaggc
     3001 ctgatcgagt tgcgagaaga gatcgcgcga aaactgagcc agacctatgg cctgcagatc
     3061 gaccccgagt cccagatcac cgtgaccctc ggggcgaccg aggctatcta ctgcgcgatc
     3121 caggcggtga tcggccccgg cgacgaggcg atcgcgttcg atcctgcgta cgactcgtac
     3181 gagccggctg tccgcctggc aggcggacgc tgcatcagat tgccgctgac ggcaccgcag
     3241 ttcggctatg actgggatgc ggttcgcgat gcgatcggcc cgcgcacccg tctaattttg
     3301 ttcaacagtc cgcagaatcc tgcgtgcacg gcggcttgcg cggaagatct cactgcgctg
     3361 gccgaagtga ttcgggatcg cgagatcttc gtgatgtccg atgaggtcta cgagcacgtg
     3421 acttacgatg gcagatcgca tcacaccatt ctgcggcaac cggagctcgc gtccaagagc
     3481 tacgtgatct tctcattcgg caagacgctg catgcgaccg gcgtacgcgt cggttactgc
     3541 gttgcgccgc ccgcactgac cacagaactc cgcaaggtgc accagttcaa cacgttcagc
     3601 atctcgagtg cgttgcagtt tgcgattgcg gggtatctgc gcgagaagcc cgaatcgtgg
     3661 cggacgctcg ccgggttctt ccaggcgaag cgcgacaggc tgcgaggtgc gctggccgcc
     3721 tcaggattcc atttgccgcc ggctcagggc acctacttcc agctgctcga ttaccgtgag
     3781 ttctcccaag tggccgatct ggcattcgct gagcggctgc tgagcgaggc cggtgtggcc
     3841 acgattccac tctcgccgtt ctatgcaaac cccccggccc tgacgctgct gcgattgtgc
     3901 atcgccaaga aggacagcac gctggatgag gcggcgcaga gactcaacgc gttcgccgcc
     3961 gctttgggca aggcggggag ctgagggcga gcggagcgcc gggagctgca atgaagaaga
     4021 ccacgcgagt gaaccatccg ccggaggtcg agctgccgcc ggacaaccgc gccctcgtgg
     4081 ccccgatcta ccagagcgtc aagttcgagt tcgattcgct aggggagacc gagcgcttcc
     4141 tgcgcgggga gcggtccggg ttcttctaca gccgcgcctc caacccgact acccggcagc
     4201 tcgagctgct gctcgcagag ctccagggcc gcgaagactg tctggtcacc gcctcgggag
     4261 tgggtgcgat tgcccagaca ctgctggcct tgacccggca gggcgatcac attgtctgct
     4321 tcatcgagac ctataacccg acacgctatc tgatccggcg cttgctggcg cggttcgggg
     4381 tcacacacac catgctctcg atcgaggatc tggcgggtgt ggagcagtcg ctcgcagcaa
     4441 agcgcacgcg gctggtgatc ttcgagagtc ccacgaatcc cgtgaccaag atcgccgatc
     4501 tgacggcgct gacgaagatg gcgcgcagtg ccggcgcgct cacggtgctg gacaacacgt
     4561 ttgccggctt tcatcagcac ggcgagtacg aggtagatct gtttctgcac agcctgacga
     4621 aatacgcttc gggcgcgggc gatgtgatgg gtggcgctgc gatcggccgc gccgatctga
     4681 tccacagcat gcgcacggac ttcggcattc tcggcggcac gctcgatccg cacgcggctt
     4741 tcctgattct gcgcggactg aaaacctatt tcgtgcgtta ccaggcgcaa tgcgcgagcg
     4801 cgatgcgcat tgccgaggtg ctggccgcgc atcctgccgt ctcccgcgtg cactatccgg
     4861 ggctgccgac gcacgcacag cacgagctgg cccgcagcca gatgcgcgag tttggcagca
     4921 tcgtgacgct cgatttgaag ggcggggcgg aggcgggtgg tcagtttgcc ggtgccctga
     4981 aactgttcgc catggcagcg agcctcggct cgaccgagtc gctggtcgtg gcgccgcaaa
     5041 tgatgggcag ccgggacctc tcccccgagc agctgcgtct ctcgggactc gccgagggca
     5101 cggtgcggct gtcgatcggc ctggaggata gcgacgatct gatcgaggac gtgcggcagg
     5161 cgctcgccag cgccggttga ctcaggcgct gcccgccacc ggcactcgat gcgggtgata
     5221 gggaagcacc tcgagcactt ctccttcccg aagccgttgc tgcacgtttc gccagaacgt
     5281 ggcggtcagg atttccccgt gcaggcgcat gaatgcggcg cgctgtgcat cattgaaacc
     5341 caggaagctg atgaaggttt cgggaaacac gtcattgtcg gcgacgtaga accaggcctc
     5401 accccgcatc tcgtcctccg gattggcggg ctggggcacg tcgcgaaaat ggcagtccgt
     5461 gacctggcag agctcatcgt agtcataaaa gatcacgcgg ccgtgacgcg tgacgccgaa
     5521 attcttcagc aacagatccc cggcgaagat gttgctatac gccagatcgc gtatgcactg
     5581 gccgtagtcg agcacggcgc gctcggcagc cgctgcggtc gcggtccgca agtacaggtt
     5641 gagcggcgtc atgcgccgtt cgatgtacat gtgatcgaag atcagatcat cgccttcctc
     5701 gtgcacggtc gcggccgtct cggcacgcag ctcctgcagc agctcgggtg cgaacagcgc
     5761 gcgcggaaag cgcagtcgct tgaattcctg cgcatcaacc agacggccgg cacggtcatg
     5821 cttgaagacg agctggtatt tcgccatgac ctcctcgcgc accacggtct tgggataggc
     5881 gaagcgatca cggatgacct tgaagaccac atcgaaggag ggcagggtga aacacaccat
     5941 caccaggcct cgctcgccgg gcgcgtgcac gaactggacc tgagtctgtt ccaggtgacg
     6001 catgagctcg cgataacgct cggtcttgcc ctgcttcgcc cgcccgagca ccgtgaacag
     6061 ctcgctgacc ggcttgcgcg gcagcatgct cttcaagaac aacacagcct cgccgacccg
     6121 atccaggtcg acatgaaagt aggacttggt gaagctgaat acgatgctca catcgccctc
     6181 tgaaagcatc acggcatcga ccagcattcc cccgtcggtg ttcttcagtg caatgacgag
     6241 cggcacgaga aattcccgtc ccacgatgcg cccgacgatg taggcgcgcg agacctggta
     6301 gaacaccgga cgaatgatct ccacgcgcag cacggcgcgc cgctcgttgc gatccttcag
     6361 atgccgtttc gtctcggtgg cgacgtgctc gacgctgcga tcgaaatcgc gccaggccga
     6421 gtggaaacgg acgtccccga gcacatcctc gaacaacagg tgcagcgagc cgtgattgag
     6481 gtaggtgttc gtccccaccg tacgggtgac cttgccaagc gggtcgagat ccgtcgcgac
     6541 gaactcgatg cccggggcga ctccgaccgt gccgaacagc cggcgcgtga tggagctgaa
     6601 gaaggtcttg gtgaactcct catccgcaag cccttcgatc atcgcggcga aatccgttct
     6661 gatgcgggac cacagctccc ggtcatgcgc ctgtgcaccg aggcgttcgc acagctgagt
     6721 gatcgtctgc ccgacgaagt ggtcgtagag ctcgatacgc tcgacggcat cgcgctgact
     6781 gcccttccag tcgcgggcat cgaagcggtc cggggcgcgc cgggtgatcg agcggaactc
     6841 tgcgttgtag cgcacgaagg cggcggcgat ctgctgcgcg cactcgtgcg ccaggctgga
     6901 tgtggagggt tgcgcagggg actcggtgat ggccatgccg gactagtcga actcggtcgg
     6961 caggcctcga tcgagctggt gccggtacag tagctcgatc tgcgagggcg tcgcccggcc
     7021 cagggacagg gacggaaggg ctttcacggg aaaatagtcc acggcatcgg tctcggtgct
     7081 cgtcgcagga ctcccgccga cccgctcgca cagaaagaac agcttgaaga tgtggtgcac
     7141 gccgggaggg tggccatggg ccggattgtt cttgtccagc aaggccgcca gtttcactgc
     7201 ccgggcacgg tagccggact cctcgaggat ttcgcgctcc acggcgccag agggtgcgtc
     7261 gttgatgtcg acccatccac ctggcagggt ccatttcccg tccgagcgtt cgcgtaccag
     7321 caggacctcg tcgccgtcga acacgcctcc gcggacgtcc accttcggag tggcgtagcc
     7381 ttcgtcatgt gaccagagat tgcgcacctg gccgaccggg atgtcgagct cggcagcgag
     7441 tatcgaggcc acgagctcct gaagctgcgt gtagcgctcg cgatcgaagg ggtccttcgt
     7501 gaaaaggaga ccattgtgcg caatggcctg taccctgcga gcccattgca gaatgtttcc
     7561 ccccgcatcg ctcgccggtg tcatgagacg cccttggcgg ctcgcgcggg cgcgcggtgt
     7621 cggttctcct ttcgcagcac cagcttcagt cccgaccagg tctcattcac agcgcaaacc
     7681 ttcacgtcga ccaggcccat gggcaaggcg atctcgcgaa tggcatcctc ggtgacgtcc
     7741 gtgcggactc ctgaggcctt tttgggccag gatacccaga tcgtgccatc cggcctgaga
     7801 cgctccatcg catgcctgca aatcttcgac agcgaggctc tgaaggtcac aaagaggtgc
     7861 acgatgtccg ttgtggcatc gatgcgggac acgagcttca cgcccggtgg caatggggcc
     7921 acgagctcca ggtaattcgg cggcgcatcc tgcaggaaga gccgactgtt ctccttgatg
     7981 ccaagcttgc gtgcgagagg tgtaccggag tagcccgcgg tcttcacggc ctggagcggc
     8041 tgcttacaga ttgcggatca gggtattacc gaactcgctc gtcgacactt ccttcgcacc
     8101 ttccatcaac cgggcgaagt cgtacgtgac gatcttctgg ccgatggttc ggtccatggc
     8161 cgagatgatc gcatcggccg cttccagcca gccgagatag cgcagcatca tttcccctga
     8221 aaggattacc gatccggggt tcaccttgtc cagattggcg tatttcggcg cggtgccgtg
     8281 cgtggcctcg aacaccgcat ggccggtgag atagttgatg ttgccacccg gggcgatccc
     8341 gatgcctccg acctgtgcgg ccagggcatc ggagagataa tcgccgttca ggttgagcgt
     8401 tgcgatgaca tcgaattcgt ccgagcgcgt gagcacctgc tgcagggcga tgtctgcgat
     8461 cgcatccttc acgatcacct tgcccgcctg cttggctgca tccatctctg tattggcctg
     8521 cttctcgccg cccgaagcgc gcgtgcgttc ccattgctcc caggtatagg tctgggcggc
     8581 gaactcgcgc tcggcaagct gatagcccca gttgcggaaa gctccttcgg tgaacttctg
     8641 gatgttgccc ttgtggacca gggtcaggct cttgcgccgg ttggccaccg catactggat
     8701 ggccgagcgg atcagccgct cggagccttc ccgggatacc ggcttgatgc cgatgccgga
     8761 actgtccggg aagcggatct tggcataggc cttggggaag gcctgcttga acaactcgag
     8821 gaacctgcgg ttctcctccg agccggtctc gaactcgatg ccggcgtaga tgtcctcggt
     8881 attctcgcgg aagatcacca tgtccaccct ctccggatgt tttaccggcg agggcacgcc
     8941 cttgaaccag cgcacaggcc gcagacagac gtacagatcc agcatctgcc gcagggcgac
     9001 gttgagcgag cggatgccgc cgcctacggg cgtggtcagc ggtcccttga tggagacgag
     9061 gtattcacgg caggccgcga cggtttcatc cggcagccag gtattcaact gcctgtttgc
     9121 tttctcgccg gcgagcacct ccatccagtg gatcttgcgc ttgccgccat aggccttctg
     9181 cacggcggcg tccatgacac gcacgctggc ccgccagatg tcggggcccg ttccgtcgcc
     9241 ttcgatgaag gggatgaccg ggttgtccgg cacgaggagc ttgccatcgc ggatcgagat
     9301 cctggcgcct tgggcagggg gcgtaatctt tgacgctgca gccgtggaca tggaccttct
     9361 cccgggtcgg cctcgcaaag gatcgggcgc gcatccggcg ccgtacccgc gaatgctagc
     9421 acaggggatt cccgagccgg tctgtgagag acttgccgga ttgcgcctgc gcgacaccaa
     9481 caatgggggc ctccaagccg cgcgcagcca catgcacaag agggtgtaat ggcatcgatc
     9541 ctgcgcgaat gcttcgctgt gcggccagtg acggaagccg acaaggaagg gggcgggagg
     9601 ctgaaacgcg ttctcaccgt ctggtcgctg acggccatcg gtcttggcgc gacaatcgga
     9661 accggcgtct tcgttctgac cgggcaggtg gcggcgaatc aggccggtcc ggccgtgacc
     9721 ttgtcgctgc tcatcgccgc cttcggcagc ggtctggccg cgctgtgcta tgccgagctc
     9781 gcagcatttc ttcccgtacc cggaagcgcc tacagctata cgtatgcgac gctcggcgag
     9841 gcggtagcct ggttcatcgg ctggaacctg ctcctcgagt acggcatgtc ggcatccgcg
     9901 gtggcggtca gctggtccgc gtacacggtc aatgttctcg agagctggca tatcaacttg
     9961 cccaaggcgc tgaccaacgc gcctttggag gccgtcaacg atcacatcgt cgcgaccggc
    10021 gcggtcttca acgtgcccgc ggcattcatc atcgtggcga tgagcgcgat ctgctacgtc
    10081 ggcatgcgcg agacagcggg catcaatacc ttcatggtgg ctttgaaggt gaccatcatc
    10141 gtgctgttca tcctggcggg gttgagttac gtcgatacgg ccaattggca cccctacgtg
    10201 ccgccggcga ccggagaatt tgggcacttc ggatggacgg gagtgatgca gggcgcagcg
    10261 atcattttct tttcctacgt tggattcgac acggcctcga ccaccgccct ggaagcccgc
    10321 aacccgcaac gtgatctgcc tctggggatc atcgcggcct tggtgatctc cgcagtgctg
    10381 tatgtcgcaa cggcgtcggt caccactggc atggtctcgt atacgcatct gggcgtcgat
    10441 gcgcccgtgg cggtggcgct ggatgcgcac ccccaacttg cgtggctcgg acggttcgtg
    10501 aagatcggcg ccatcgtggg gatgacctcc gtggtgctcg gctcgctgct gggccagccc
    10561 cggatcctgc ttgccatggc cgatgatggt ctgctgcccc cggcgatcgc tcgctgtcat
    10621 ccgcgtttca aaactccaca catcgcgacg gtcgtgacgg gcgttgtctt cgcgctcatc
    10681 gcgggcgtgt ttccgctgga catcctggcg gatctgatct ccatcgggat cctgctggcg
    10741 ttcgtggtcg tctgtatcgg agttctcatc atgcgccgga cccggcccga tgcgccccgg
    10801 ccttttcggg ttccctgggc tcccttcacg tgtgtcctgg gagcggtctt ctgcagcgcc
    10861 atggcgtgct ttctgtcgaa aggcacctgg tggagactgg tgttctggac cattctcgga
    10921 tttgcgattt acgcgttcta cggtttcaag cacagccgcc tgcgcaagag atgacgtgca
    10981 ctctgacgag cactccggcg gcggacggat tctggatgcc ggcggagttc gagccccatg
    11041 ccggctgctg gatgctgtgg ccggagcggc ccgacaactg gcgtgaagcg gcccgcccgg
    11101 cacaactcgc cttcgtcgca gtcgccagcg cgatcgcgca attcgagccg gtgacgatcg
    11161 gcgcctcggc ctctcaatac accactgcgc gtgcgctgct gcccgagcac attcgcctgg
    11221 tcgagctggc gcacgacgac agctggatgc gtgatgtcgg gcccaccttc ctcgtcaatg
    11281 cgcgcggggc ggtacgcgga gtggactggc cattcaacgc ctggggcggt ctgaacggcg
    11341 gcgtttattt tccctgggat caggatgacc tcgtcgcgag aaaggtgctg gagatcgagg
    11401 cgcgcgagcg ctaccgggcg cccctgatca atgagggcgg cgcgctacac gtggacggcg
    11461 agggcactgc gctggtgacg gagcagtgcc tgctgaacgc gaaccgcaat ccctcgctcg
    11521 accgcagcca aatcgagaca gcactgcgcg attatctagg ggtgagctgc gtcatctggc
    11581 tgagtcaggg tgtatgcaac gacgaaaccg atggacacat cgataacctc gcctgcttcg
    11641 tgcggccggg cgaagtgtgt ctgggctgga cggacgacag aaacgatcct cagtacgccc
    11701 tctcgctgga tgcctgggag cgcctgcagg aagcacgtga cgcccgcggc aggcggctca
    11761 aagtgcaccg cctgcccatg ccaggccctc tgcgcctgca agcccgcgag gctggcggag
    11821 tggtcgcacg cgagggcacg aaaccacgac tcgccggcgt gcgcctcgcg ggcagctaca
    11881 tcaatttcta catcgccaac ggcggcatca tcatgccgct gctggatcgg cgtacggatc
    11941 gggccgcggc ctccaggctc aagcgcctgt tcccgaagcg gcgagtcgtg ggggtagcag
    12001 ctcgcgagat cctcctggga ggcggtaaca tccactgcat tacccagcag gtaccttgcg
    12061 ccggccagaa gcggaagtcg cctctctgag gggtcgcaac gcccagtacg tgtgcgtgtg
    12121 atgcacgaca cggttcgcgg gggaacttgc gctattgtcg gttgatgcgt cttctgcttg
    12181 ttacgctggc tgtctcggct gcggcgttcg cgggtgcgag cggcggttcc gctgagttca
    12241 accctgtgcg gacgcatccg cacgcccagg cgcacgcagg cattcatcgc atcatcatca
    12301 agctcaggcc ggcttccggg tcggcggcgg cttcctcaaa actgcagatg cgtgcggcgg
    12361 atcgtagcgc cgccctggcc tcccgcaccg gagttacgtt aaaagccact cgcccgatca
    12421 ctgcagacct gcaggctctg gaggtcgttc ccgccgtggc cgaagaaggt atcgaagcga
    12481 cccttgcacg gctgcgggcc gacagtgagg ttgaatacgc cgaggctgat cagaggcggt
    12541 atatccacgc agtgccgaat gactcgttgt atgccacaca acagtggtac atgcagagct
    12601 ctgcggctac gcccagcgcg gtcaatgcgg aaggcgcgtg ggacacgacc aacggaagcg
    12661 caggcctggt gatcgccgat ctggacaccg gaatccgttt cgatcatccg gatctgctgt
    12721 gggccgggca gggctcgggc ggtcgcctcc tgcccggcta cgatttcatc acggacccct
    12781 tcgtggcaaa cgatggagac ggctgggacg ccgacccgtc cgatccggga gactgggtga
    12841 cgagcgcgga tctgaaacag actgagtgca gcaacatcac gacggtttcc aacagctcct
    12901 ggcacggcac tcgtacggcc ggaatgctgg gtgctctgac caacaacagc gccggcatcg
    12961 ccgggatgac ctggaacgcg tggatcctgc ccgtgcgcgt gctgggcaag tgcggtggac
    13021 tggattcgga catcatcacc ggcatgctgt gggcggccgg cgtgcatgtg gccggcgctc
    13081 ctgacaaccc gtatccggcg aggatcctga atctgagtct ggggggcacg ggcgcctgtc
    13141 ccaccagtta cgtcgatacg atcacccagc tgagtcagct cggtgtgctg gtcgtggttt
    13201 cggcaggcaa tgagggcggt ccggtcgatg cgcccgccaa ttgtccgggg gtcgccggtg
    13261 tcgcaggact gaggcacgcc ggcaccaagg ttggcttcag cagtctcggg cccgagctgt
    13321 cgctgggcgc gccagccggg aactgtgtca acacggcgcc tggatcagcc tgtatttact
    13381 cgatcggaac gactttcaac ctgggcacga ccacgccggg tcagaacagc tataccgacc
    13441 agctcgccaa cccgaatctc ggcacgagct tctcggcacc gatcgtctcg ggcattgctg
    13501 gtctcatgct tgcggtcaac ggcaatctcg gctccgcgca gctcatcgcc cggcttaagg
    13561 aaggagcgct gcctttcccg cagacctctc tcggagagac cccacagccg ccggcttgtc
    13621 atgtgccatc aggcacctcc gatgtgcaag acgccgagtg catctgcacg accgacggac
    13681 gcacctgcgg cgccggcatg gcaaacgcgg cgggcgctct agcggccgcg cttcgaccga
    13741 tcgccgctgt acaggctgct gcgacggtaa tggctggtca gacggtatcg atggatgctt
    13801 ccggcagcgg cgctgcgtgc gggcacaata tcgctagcta tcagtgggtc agcagcgatc
    13861 cgacacatca tcctgtcagc agctcgaccg gaccgacgac ttctgtggtc gcacctgcca
    13921 gtgggtccgt cacgatcagc ctcctcgtga ccgacgaggc cggcagaacg gacagcgcaa
    13981 ttatcgtcgt gagtcccact tcaacgacga cgagcgcgcc agtgagcgct ggcgccaatg
    14041 cctgcctcaa aacgattgcg gtggcctcac ctatcacgct ctccgtccag cctgccagcg
    14101 ctaccgtccc gagc
//
LOCUS       DASRHN010000029        21073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1162564, whole genome shotgun sequence.
ACCESSION   DASRHN010000029 DASRHN010000000
VERSION     DASRHN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162564"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="VFB37_01030"
     CDS             complement(<1..1613)
                     /locus_tag="VFB37_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO21053.1"
                     /translation="MPQFFIDRPVFAWVLAILITLAGLISVTRLPIEAYPAVAPPQII
                     VSATYPGANASTVEATVTQVIEQQLTGIDNLLYFNSQSGSDGSSQITLTFENGTDPDI
                     AAVQTQNRVSLAQPRLPAEVTQQGIRIAKANAGFLAAIALRSAPGGPSADELSNLIAS
                     RVLDEIQRLPGIGSATQFGSEYAMRIWLNPDKLHAYGLSAATVLDIVRQQNVQVASGS
                     IGAEPAVPGQEVNASVVAEGRFTSAEQFENILLRTEPNGTSVRLKDVARVSLGQYQYG
                     FESRLDDKPVAAFGIQLLPNANALDAMRAVKARMAELAHSFPAGVTWFFPYDSTAFIR
                     VAIRDVLITLVEAVALVFIVMLVFLQSFRATLIPTLVVPAALMGALVGMSAVGFSINQ
                     LSLFAMVLAIGIVVDDAIVVVEAAERIMREDHLSPREATRKAMGQITGAIVAITLVLA
                     AVFIPSALQTGSTGVIYRQFALTIALSMGFSAFLALSFTPALCATLLRPEHLRGNFVF
                     RWFNRTYEITRRSYVHRVFQSVTHTPRWMA"
     gene            complement(1641..2825)
                     /locus_tag="VFB37_01035"
     CDS             complement(1641..2825)
                     /locus_tag="VFB37_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO21054.1"
                     /translation="MSRQWLSRLASLAAIALLACACHRSGQSAPATGPPPQVGVITVH
                     AAPIPLTRELVGRLSATRVADVRARVAGILLKRLYQEGTDVKQGQPLFQIDPAPLKAA
                     LDAATAALAQAQATATNAHVAAQRTRDLVPGGLVSRSDLDNAQATERSSAAAVQQAQA
                     NVEAARINLGYARVTAPIAGRAGQQAVTEGALVGQGTATLLTTVEQLDPIYANFDQPA
                     VEVERLRRSQSSGDITLVQPNKAVVQLTLPDGTPYGRSGLLDFSDVSVDPTTGAVALR
                     ALVPNPDEQLLPGMYVNVRLTLGTLNHAYLVPTAALQRDADGPYVLAVGSDDKVLQKR
                     VVSDTSRSQNWIVTGGLNDGDRIIVTGIPSARPGATVVAVPYSTQNETAANSDEPAPA
                     HP"
     gene            2919..4121
                     /locus_tag="VFB37_01040"
     CDS             2919..4121
                     /locus_tag="VFB37_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZO21055.1"
                     /translation="MPPELNKAATPEPGPAHTRRRWLLGVTASFVVIGLIAGAWWWLY
                     GRHYQSTDDAYVAGDLVSVMSQVTGTVIGINADETDLVKAGQELVRLDGTDTEIALRD
                     SEARLARAVRQVRTVFATRDQLEALVAQRRADLQRAQADVQRRRNLTASGAVSMEEAA
                     HARDAVSGAQDALLAAEKNLAASDALTGQTGVSDNPDVEAAATDVERAYLNARRTSVR
                     APVSGYVAKRAVQLGERISPGVALLAIVPLDRLRIEANFKEVQLNQMRIGQPVRVVAD
                     LYGSRVEYRGTIEGLGLGTGAAFALLPAQNATGNWIKVVQRVPVRITLDPQQLVEHPL
                     RIGLSTRVRVDVRDASGPQLARSARVKPVLATTAYDIDKQEILARIAQIIQENTLQGS
                     GQVASRQR"
     gene            4471..5847
                     /locus_tag="VFB37_01045"
     CDS             4471..5847
                     /locus_tag="VFB37_01045"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZO21056.1"
                     /translation="MKLPHTTAVRVVIFSGLTWMTAAAASAATDPGVRPGVSAGDPVP
                     GLTADESSLFFAGQTAFAEVEGLGDGLGPRFNLDSCTGCHAYPARGGSSPPVNPQSTV
                     ATAMGAHNSLPVFISANGPVREARFKFTSSGARDGGVHSLFVISGRTDSTGNATQCTA
                     VQEDFNTQWSRGNVSLRIPTPTYGTGLIESIPDTTLVSNLAANSRQKSQLGISGHLNT
                     NGNDGRVTRFGWKAQNVSLLMFAGEAYNVEMGITNELFPTERDLNPTCQTAATPNDTT
                     GKNGLANMSDIVNDIEKFALYMRFLGAPVASTTLPGGATSISRGQRTFSNVGCALCHT
                     PVLTTDTTAVAALASKPVPLYSDLALHHMGPKLADNILQGAAGPDEFRTAPLWGLGQR
                     VYFLHDGRTSDLLQAIEAHSSADAGTGFHQPGTTSAPPSEANQVVGNFNGLSDGAKQD
                     VLNFLRSL"
     gene            6026..6658
                     /locus_tag="VFB37_01050"
     CDS             6026..6658
                     /locus_tag="VFB37_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21057.1"
                     /translation="MTLALVSAPGGADPSSGPAQEQWPGYEVAPFAGLSLGGGFDVEN
                     GSSREAGQHVGLGTRASFAIALDLRADEAAQYELFYGRESMLLRGDLSFEAAPVVVEY
                     LHAGGLLLLSEDLPVKPYIVAGLGLMRLSPDAPGSSDTRFSASGGMGLRWPVNRHVAL
                     RVEGRGLASLINADTAIFCRSDQSGLLCRIHGQGRTFLQGMLLAGVAVNF"
     gene            complement(6669..7136)
                     /locus_tag="VFB37_01055"
     CDS             complement(6669..7136)
                     /locus_tag="VFB37_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO21058.1"
                     /translation="MDKLDRKILDLIQKDAGLTAAELADRVGLSKAPCWRRLKRLEEA
                     GIIKQTVALLDARALNVGTTVFVTLKTGNHSEAWFDRFVRAVRDIPEVTEIHRMSGDV
                     DYLMRIVVPDIDAYDVVYKRLIAAVEFQDVSASFALETIKYTTALPLSYVQLA"
     gene            7247..7498
                     /locus_tag="VFB37_01060"
     CDS             7247..7498
                     /locus_tag="VFB37_01060"
                     /inference="COORDINATES: protein motif:HMM:NF040386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HZO21059.1"
                     /translation="MSLGRLFTEHPASVGESYIAHLLRAAWFGSRMMLAGGACLVHAI
                     FPFLFVKTGSNAIAELHTAMVTGRRPTAGPTLESRRVPS"
     gene            complement(7534..8385)
                     /locus_tag="VFB37_01065"
     CDS             complement(7534..8385)
                     /locus_tag="VFB37_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZO21060.1"
                     /translation="MPSGADTTNLALFCDFENIALGVRDARYAQFDINRVLERLLLKG
                     SIVVKKAYCDWERYKEFKSGMHLAGFELIEIPHVKQSGKNSADIRMVVDALDLCYTKT
                     HVDSFVIISGDSDFSPLVSKLRENNKDVIGVGVKNSTSDLLIANCDEFIYYDDLVREQ
                     PRARRTRKKAPASEPSAQTAEPGRGGESRGESEHREEDHKQEAFDLVMATLDALVSER
                     GAEDKIWGSMVKQALKRQRPGFNESYYGFRSFNKLLEEAAQRRLITLERDEKSGGYLV
                     HSSAESA"
     gene            8550..8783
                     /locus_tag="VFB37_01070"
     CDS             8550..8783
                     /locus_tag="VFB37_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO21061.1"
                     /translation="MASENIAAIFGKVLRELRNARGISQEALALSADVDRTFVSQMER
                     GIRQPTLTTLCKLSGALEIQPSTLVAKVERLMR"
     gene            8898..9503
                     /gene="folE"
                     /locus_tag="VFB37_01075"
     CDS             8898..9503
                     /gene="folE"
                     /locus_tag="VFB37_01075"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZO21062.1"
                     /translation="MADKQRRKVSRKAAEEAVRTLLRWAGDDPEREGLADTPKRVANA
                     YRDWFSGYDIDPAEYLRRTFEEVGGYDEMIVLRDISFESHCEHHMAPIIGRVHVGYLP
                     TNKVVGISKIARVVEGYARRLQVQEKLTAQIANCINSVLKPRGVGVVIDATHECMTTR
                     GIHKRGVSMVTSRMTGTFREDARTRMEFLQFVDISGVREVS"
     gene            complement(9577..10710)
                     /locus_tag="VFB37_01080"
     CDS             complement(9577..10710)
                     /locus_tag="VFB37_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21063.1"
                     /translation="MISRLSRPAALCALSGLLTLAAGTARADVTVVNTIAVEGIGAMA
                     FGNLKGTTRTTISGDKSRMDSDIQMQSRLVRLLAHNAVGPSATIVRLGDDRIYHLNIN
                     KKEYTETTFEQMREQMNKLADQMNQAGQKEKEEQQGPSAVDESKCEWLPPRLDVKKTG
                     EKAQIAGYDAERLTLLASQPCKDKETGAICEITLALDEWLASGFTENSEAQKYHKAYA
                     AKLGFDVSSSQDFSQRAQAMFGRYKGIWSELVSKMQGMKGYPVKSSFTLALGGEQCKN
                     AQQAKSSEESSGNSSGGGGGTANLAGQVAGKLGSLFHKKKDDEQAAQPAQPAQPADAP
                     PVPAGDVVMMTVSTQLVSVTTDVATPNAFEVPADFKKRELKTQ"
     gene            complement(10904..12733)
                     /gene="typA"
                     /locus_tag="VFB37_01085"
     CDS             complement(10904..12733)
                     /gene="typA"
                     /locus_tag="VFB37_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZO21064.1"
                     /translation="MNRPIRNIAIIAHVDHGKTTLVDCLLKQSGTFAAHEKLGERIMD
                     SNDIERERGITILAKNCAIEYGGTRINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLKAIVVVNKIDRPGARPAWVIDQTFELFDKLGASDEQLDF
                     PVIYASALQGWASTDYTERRPDMAALFEAILAHVPPPAAEAAKPLQLQISTLDYNSYV
                     GRIGIGRIRRGAVRPGQVVALRFGEEDRGTAKIGQVLTFKGLQRAPVDEATAGDIVAV
                     TGIEAVNIGLTVCAPESAEGLPPIRVDEPTLAMNFQVNTSPLAGREGKYVTSRQLRER
                     LYRELQSNVALRVEDTDEPDVMRVSGRGELHLTILIENMRREGYELAVSRPQVLTRVV
                     ETEVHEPYEALTVDIEESHQGAVMEALGVRRADLTDMVIDGSNGRARLEYRVPARGLI
                     GFQGEFMTLTRGTGLMSHIFDGYAPVKGEIPDRHNGVLVSNEAGEAVGYALFNLQERG
                     RLFVSPGEKLYEGMIIGIHSRENDLVVNPIKTKKLTNIRAAGKDEAILLTPPIELTLE
                     YAVEFIADDELVEVTPISIRIRKRYLREQERKRAARSEPALTV"
     gene            complement(12937..13368)
                     /locus_tag="VFB37_01090"
     CDS             complement(12937..13368)
                     /locus_tag="VFB37_01090"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HZO21065.1"
                     /translation="MDIAHLIQSALAPVFLLSGVGVTLNVMTGRLTRVVDRARSLEGK
                     LLERPAETREIEASLRVLAKRARYIYAAIAMCGCSAVLVALVVITLFANAFFGSGFAA
                     TIALLFVGSMIFITGAYLAFLVEVRIATSHLRIGPQKRQGT"
     gene            13517..14701
                     /locus_tag="VFB37_01095"
     CDS             13517..14701
                     /locus_tag="VFB37_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HZO21066.1"
                     /translation="MQLLRRNLSPGGILAATPGDRARARSYTAIYGRDAAVCAIGMAL
                     SGDEILRGEARAGLRTLAEHQAPNGQIAKFVDLSRQEADFWYLGCIDSTLWWLIALAV
                     LDRLAHKTERSGLRRHYARHIERATQWLLAQEHQRFFLLQQNEASDWADIMPRSGFVL
                     YTNALWYWVKRSYGLPHAAATYGNFNGLFHPFSAGLAPYRRARLLNDYVLRRGRDRSL
                     YLSFVNFSFFGEEGDVFGNVLAVLMGLADPRAMRRTLRALQRAHIGAPYPVRAVIQPI
                     RQSSPLWRPYMVRHRQNLMWQYHNGGIWPMVGGFWIAALAAAGQLGHARTELLGLARA
                     CSINDWGFTEWLHGRTLAPRGMPGQSWNAAAFLIAQHAVETGKNVFDLGPRRSVLRER
                     LD"
     gene            complement(14951..15316)
                     /locus_tag="VFB37_01100"
     CDS             complement(14951..15316)
                     /locus_tag="VFB37_01100"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HZO21067.1"
                     /translation="MAYVDGFVVPLPNGKEGEYRQMAETFAKKAQAEGAIGTIEALGD
                     GLEHGHTTDFFKAVKAQDNENVVFSFILWPSKDVRDAAWKKLMADPEMQPGVQPMPFD
                     GKRMFWGGFKPLLNTLDKS"
     gene            complement(15712..16155)
                     /locus_tag="VFB37_01105"
     CDS             complement(15712..16155)
                     /locus_tag="VFB37_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21068.1"
                     /translation="MIRTTIFLGMCLAASSLALSARAQDVRQYTDGPVTEMDYIRVEY
                     GHFDEYIDWLNSTWKPTMEATKKAGLILDYKVVRATPKAPDQPNIFLMITFKNMAAAL
                     DKEVELEDVANKVIGSTEVQNKARVGRNQYRTVVGIEYVREVILK"
     gene            16735..17691
                     /locus_tag="VFB37_01110"
     CDS             16735..17691
                     /locus_tag="VFB37_01110"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO21069.1"
                     /translation="MLDDLRALVEFAHAGSIAGAADRLFRTPSAITRQVQRLEAALGA
                     ELLDRSVKPARLNSLGARVLEQARDLLQRTEALKSLTASDAEPHGVLRIGLSHPLAER
                     TLIDPILALTDKYPKVRLRLVSELTVELYRRLLAGELDVAVVLLPEGKAAPPPLLTNI
                     IATDRMEIVQGAAGDLDGDWKSLGGAPWVLNPPGCPLRAALLDRMERDGCSPMIAAEV
                     HNMHLQLAFVQAGYGVGLLPARFIARNRSIDTVKLLRPPSFDLRPSVAIVRAGQLGAL
                     GKAVELLEHGLRQLLEAADTAKPLPRHRRGVPANRRAARRPA"
     gene            complement(17692..18129)
                     /locus_tag="VFB37_01115"
     CDS             complement(17692..18129)
                     /locus_tag="VFB37_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZO21070.1"
                     /translation="MCRKGGRPRSSEAAPNGCSISDPFHRAAASGADVAREPADVFAH
                     CATVFLMLSDGEAIDAVLDRCGAAFAPRVAGRTVVHMGTTSPAYSRELEADIRSVGRR
                     YVEAPVSGSRKPAEAGQLVAMLAGEADALANVRRPCSALTEGQ"
     gene            complement(18119..18643)
                     /locus_tag="VFB37_01120"
     CDS             complement(18119..18643)
                     /locus_tag="VFB37_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21071.1"
                     /translation="MIASVAGLTVLAMTATLADALVEKAMAQAPDADCALPSEPEGIP
                     AALDAAISGPADKDRACMKVLLISEARVMFVSRGTDGAPTYALQTLDDWITRVKARGH
                     GLLEEKQLKFHIERYGNIAHLWSSYGLHSDGKPVACGINSIQAIKEAGGWRVTSIMVQ
                     AESASAPLPKKYLP"
     gene            complement(18712..19476)
                     /locus_tag="VFB37_01125"
     CDS             complement(18712..19476)
                     /locus_tag="VFB37_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21072.1"
                     /translation="MKTIAMLIAAVALAYAAIAMDNAGNSRAATTAVADPSRPHSDTS
                     RDANRKPVQTLEFAGAKSGDKVADYVADTGYFTRLFATVVGSNGHVYAVQPTGFFKYP
                     NFVKGVTDLQSYPVTHPNVTVTIAAALEGLKFPEKLDLLWISQNYHDLHDEFMGPLDI
                     AAFNKAVYGALKPGGLYVILDHSAAQGAPANVTETLHRIEASTVRREVEAAGFEFDSE
                     SSILANPADPRTAKVFDPTIRGHTDQFLLKFRKPKH"
     gene            complement(19593..20501)
                     /locus_tag="VFB37_01130"
     CDS             complement(19593..20501)
                     /locus_tag="VFB37_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZO21073.1"
                     /translation="MKYAFHIVDVFSSTPFGGNQLAVLHEAAGISTEGMQKIAREFNF
                     GETTFVLPKNDPANTCRVRIFSPRAELDFAGHPTVGTACVLLMKENERLSDPIRLILE
                     ENIGPVTVDVAQRKGSLHGTLTLSAKIETPTGAPSPTDLAAVLSIEPAEVRQVFFAGV
                     GVRFCFAQLSSNEVVDRAVINRVAWAATLARAWSPQVFFFSGDLRDGGKLYARMWAPA
                     LGVEEDPATGSACAALVGAMASRPGFGGRTYRLSIQQGVSMGRRSEIEAEARKSGSVV
                     TSVSVGGATAYIASGEIEVPPSALVP"
     gene            complement(20524..>21073)
                     /locus_tag="VFB37_01135"
     CDS             complement(20524..>21073)
                     /locus_tag="VFB37_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HZO21074.1"
                     /translation="RESACLAIPTRRMGGPHVLSGTAVDVGEPGRARYTGQAAAGLNF
                     LNVYHRSPQEFAMSKTAVTSRELAPPAGPFSQAIEVGGFLYVSGQVGQDPATGKVVEG
                     GIEAETERVLRNLAAVLQAAGRSFDHVARAAVYLTRMSDFAAMNGIYAKYFDQPFPAR
                     TTIAVAALPLGACVEIDLVVKA"
BASE COUNT         3775 a         6663 c         6700 g         3935 t
ORIGIN      
        1 gccatccagc gtggcgtgtg ggtcaccgac tggaagacgc gatgcacgta cgatcgccgg
       61 gtgatctcgt aagtgcggtt gaaccagcgg aagacgaagt tgccccgcag atgctccggt
      121 cgcagcaatg tcgcgcacag cgccggggtg aaactcaagg ccagaaaggc cgagaacccc
      181 atggagagcg caatcgtgag ggcgaactgt cggtagatca cgcccgtgct accggtctgc
      241 agggcgctcg gaatgaatac cgcagccagc accagtgtga tggccacgat cgccccggta
      301 atctgaccca tggccttgcg agtggcctcg cgcgggctca gatggtcctc gcgcatgatg
      361 cgctccgccg cctccacgac gacgatcgca tcgtccacga cgataccgat agcgagcacc
      421 atggcgaaca gactgagctg attgatggaa aagccgacgg cgctcatgcc gaccagcgca
      481 cccatcagcg ccgccggcac gaccagggtt ggaatcagcg tggcgcggaa gctctgcaga
      541 aacacgagca tcacgatgaa aacgagcgcg actgcttcca ccagggtgat gagcacgtcg
      601 cgaatggcca cccggatgaa cgcggttgaa tcgtacggaa agaaccaggt cacccccgcc
      661 ggaaagctgt gcgccagctc ggccatccgc gccttcaccg cgcgcatcgc atccagggca
      721 ttcgcattgg gcaggagctg gatgccgaag gccgccaccg gcttatcatc gaggcgagac
      781 tcgaagccat actgatactg acccagggaa acgcgggcca cgtccttcag gcgcaccgat
      841 gtgccgttgg gctcggtgcg cagcaggatg ttctcgaact gttccgcgga ggtgaagcgg
      901 ccttccgcga cgaccgaagc attgacttcc tggcccggta cggccggctc ggctccgatc
      961 gagcccgatg cgacctgcac attctgctgt cggacgatgt ccaggaccgt cgccgccgag
     1021 aggccatagg catgcagctt gtcgggattc agccagatac gcatcgcgta ttcggagccg
     1081 aactgggtgg ccgagccgat gccgggcaga cgctggatct catcgagcac gcgcgaggcg
     1141 atgaggttgc tgagctcatc ggcgctgggg ccgccgggag cggagcgaag cgcgatggca
     1201 gcgagaaagc ccgcattcgc cttggcaatg cggattccct gctgcgtgac ctcggcgggc
     1261 aggcgaggct gcgccagcga caccctgttc tgggtctgca ccgctgcgat gtccgggtcg
     1321 gttccattct cgaaggtcag cgtgatctgc gaggacccgt cagagccgga ctgcgagttg
     1381 aaatagagga ggttgtcgat cccggtgagt tgctgctcga tgacctgggt gacggtcgct
     1441 tccaccgtgc tcgcgttggc gcccggatag gtggcgctca cgatgatctg cggcggagcg
     1501 acggcgggat aggcctcgat cggcagtcgc gtaaccgaga tcagacctgc cagggtgatg
     1561 aggatcgcca atacccaggc aaagaccgga cgatcgatga aaaattgagg catgcaggga
     1621 tgaaatcgac tcgccgcgct tcagggatgc gccggcgcgg gctcgtcaga gttcgccgcg
     1681 gtttcattct gcgtggaata gggcaccgcc acaaccgtgg cacccgggcg tgcgctcgga
     1741 atgcccgtca cgatgatgcg atcgccgtcg ttcagcccgc ccgtcacgat ccagttctga
     1801 ctccggctcg tatcggagac cacccgcttc tgcaggacct tgtcatccga gcccacggcc
     1861 aggacgtagg gaccatctgc atcccgctgc agggcggcgg tcggaacgag gtaggcgtgg
     1921 ttgagggtac ccagggtgag gcgcacgttc acgtacatgc ccggaagcaa ttgctcatcg
     1981 ggattcggaa ccagggcgcg aagtgccacc gctccggtgg tcgggtcgac cgaaacgtcg
     2041 gagaaatcca gcaggcccga acggccataa ggtgttccat cgggaagcgt gagctgcacg
     2101 accgccttgt tgggttgcac gagcgtgatg tcgccagagg actgcgagcg gcgcaaccgc
     2161 tccacctcca ctgccggctg atcgaagttg gcatagatcg ggtcgagctg ctcgacggtc
     2221 gtgagcagcg tggcggttcc ctgcccaacg agcgcacctt cggtcacagc ttgctgaccc
     2281 gcgcggcccg caatgggcgc ggtgacccgg gcatagccca ggttgatacg agcagcttca
     2341 acatttgcct gagcctgctg aaccgccgcc gcagaggagc gctccgtcgc ttgggcattg
     2401 tcgaggtccg atcgcgatac gaggccgccg ggcacgaggt cgcgcgtccg ctgagccgcg
     2461 acgtgcgcgt ttgtcgcggt ggcctgggcc tgggcgaggg cggcggtcgc ggcatcgagt
     2521 gccgccttga ggggcgctgg gtcgatctgg aagagcggct gcccttgttt gacgtcggtt
     2581 ccttcctggt ataaccgctt gagcaggatt ccagcgaccc gcgcacgcac atccgccacg
     2641 cgtgtggcgg acagccttcc gacgagctcc cgggtgagcg gtatcggggc ggcatgaacc
     2701 gtgatcaccc cgacctgcgg aggcggtccg gtggcaggcg cactctgccc gcttcggtgg
     2761 caggcgcacg ccagaagcgc gatcgccgcg agcgacgcga gccgtgaaag ccattgcctt
     2821 gacaaaagag tcccttccag tgagaaataa ctgccgaggc agtcatctta ccgagtgcca
     2881 tcgcatctgg aaaagggcct cgtgagccgg aatccgccat gccccccgaa ttgaacaagg
     2941 ccgcaacgcc ggaacccggg cctgcgcaca ctcgtcggcg ctggcttctg ggggtgaccg
     3001 catctttcgt ggtcatcgga ctgatcgccg gcgcctggtg gtggctatac gggcgccact
     3061 atcagagcac ggacgatgcg tacgtcgccg gagatctggt gagcgtcatg tcccaggtga
     3121 ccggcaccgt catcggcatc aatgcagatg agacggacct ggtgaaggcc ggccaggagc
     3181 tcgtcaggct cgatggcacc gatacggaga tcgcgttacg ggattccgag gcgagacttg
     3241 cccgagcggt ccgccaggtc cgtacggtgt tcgccactcg ggaccagctt gaggccctcg
     3301 tggcgcaacg ccgcgccgac ctgcagcgcg cgcaggcgga tgtgcagcgg cgcaggaacc
     3361 tgaccgcaag cggcgcggta tccatggagg aggcggcgca cgcacgcgat gccgtgagcg
     3421 gcgcgcagga tgcgctgctt gcagcggaaa agaatctcgc cgccagcgat gcactgaccg
     3481 gacagacagg agtgtcggac aacccagacg tcgaggccgc ggccaccgat gtcgagcggg
     3541 cctacttgaa cgcgcgccgc accagcgttc gcgctccggt gtccgggtac gtagccaagc
     3601 gcgcggtgca gctcggggag agaatcagtc cgggtgtggc cttgctcgcg atcgtgccgc
     3661 tcgatcggct tcgcatcgag gcaaacttca aggaagtcca gctcaaccag atgcgcatcg
     3721 ggcagccggt tcgcgtcgtc gccgatctgt acgggagccg cgtcgagtat cgcggaacca
     3781 tcgaggggct gggcctgggg acgggcgccg ccttcgcgct gcttccggca caaaatgcca
     3841 cgggaaactg gatcaaggtc gtacagcggg tccccgtccg aatcacactc gatccacagc
     3901 agttggtcga gcatccgctg cgcatcggct tgtccacgcg agtcagggtc gatgtgcgcg
     3961 atgcctccgg cccgcagctc gcacgctccg cacgagtgaa acccgtgctc gcgaccacgg
     4021 cctacgatat cgacaagcag gaaatcctgg ctcgcatcgc gcagatcatc caggagaaca
     4081 cgctgcaggg gtcggggcag gtggcctcgc ggcagaggtg aacagcagct cgcagtgccc
     4141 cgggctgcgc ggtaatggcc cgccccaaca tcggctgagg caagcggcaa gctgacagcg
     4201 gccccggggg cgtccgcggc ttgcgacaca ccgttctgcc actaatcaac gacttgtcag
     4261 tgcagagcgg atttgcgtcg gccctgcgcg acaagtcgcg tgacccgcga tggcccccgg
     4321 ccgtgcacgc tcgatcgcac ggagttggtt tggatcacga attcaaacag ttggacgggg
     4381 acggctgtat gcgcgtgcat acacggcatg gcctttgctt tgactccagc cgcacaggcc
     4441 gtcgcacgca cgagcctgca ggggggagtc atgaaactac cgcacacgac ggccgtgcgt
     4501 gtcgtcattt tttcggggct cacctggatg accgcggcgg ccgcgagcgc agcgaccgat
     4561 ccaggcgttc gcccgggcgt gagcgctggc gatcctgtcc cggggctgac tgccgatgag
     4621 agctcgctgt tcttcgcagg gcaaaccgcg ttcgcagagg tcgaaggtct gggcgatggg
     4681 ctcggaccgc gattcaacct cgacagctgt acaggctgtc atgcctatcc ggcccgcggc
     4741 gggtcctcgc cgccggtgaa tccacagtcg accgtcgcca ccgcgatggg cgcgcacaac
     4801 agtctgccgg tgtttatcag cgcgaatgga ccggtgcgcg aggcccgctt caaattcact
     4861 tccagcggcg cacgcgatgg gggcgtgcat tcgctcttcg tcatcagtgg ccggacagac
     4921 tccaccggaa atgccactca atgcaccgcc gtacaggaag atttcaacac gcagtggtcg
     4981 cggggcaacg tctcgctgcg cattccgaca cctacctacg gcacgggact catcgagagc
     5041 atcccggata cgaccctggt gagcaatctc gctgccaata gccgtcagaa gtcgcagttg
     5101 ggaatctccg ggcatctgaa cacgaacggc aatgacgggc gcgtcacgcg cttcggctgg
     5161 aaggcgcaga acgtctcgct gctgatgttc gccggcgagg cctacaacgt cgagatggga
     5221 atcaccaacg agctctttcc gaccgagcgg gatctgaatc caacctgtca gacggcggcg
     5281 acgcccaatg acacgactgg aaagaatggc ctggcgaaca tgagcgacat cgtcaacgac
     5341 atcgaaaagt tcgcgctgta catgcgtttc ctgggcgcgc ccgtggcatc caccaccctc
     5401 cctggcgggg ccacctcgat cagccgtgga caacggacct tcagcaatgt cggctgcgcg
     5461 ctctgccaca cccccgtgct caccacggac accactgccg tggcagctct ggccagcaag
     5521 cccgtgccgc tttactcgga cctggccctc caccacatgg gcccgaaact ggcggataac
     5581 atcttgcagg gcgctgccgg ccccgacgag ttccgtaccg cgcctttgtg gggcctcggt
     5641 cagcgagtgt atttcctgca tgatggacgc accagcgatc tgctgcaggc catcgaggcc
     5701 cattcgagtg cggatgcggg cactggcttt catcagccgg gcacgaccag tgcgcccccg
     5761 tccgaggcca atcaggtcgt cgggaatttc aatgggctga gcgacggcgc caagcaggac
     5821 gtcctcaact tcctgcgaag cctgtaatcg ctccgtagcg gcgcaacgga gcagcggact
     5881 tcgaggtgag ttgcgctgaa gtgtggcggc ccgggaacgc tttcgactga ttgaactggg
     5941 aacgtgcgtg gtgcggatcc tctccttatc atcgatgcgc gtggtcgatt gcagtgagga
     6001 ctccgtgcgg cagttggctt tagcgatgac actcgcgctg gtgagcgcac cggggggggc
     6061 cgacccttct tccggaccgg cgcaggaaca gtggcctggc tacgaggtgg ctccctttgc
     6121 cggcctgagc ctgggcggcg gctttgacgt ggagaacggc tcctcgcgag aggctggcca
     6181 gcatgtcggc ctgggtacgc gcgcctcttt cgccatcgcc ctcgacctga gggcggacga
     6241 ggccgcgcaa tacgagctgt tctatggacg cgagtccatg ctattgcgcg gtgatctgtc
     6301 cttcgaggct gcaccggtgg ttgtggagta tctgcacgcc ggcggcctgc tgctgttgag
     6361 tgaggacctg ccggtcaagc cctacatcgt tgccggcctc gggctgatgc gtctcagccc
     6421 ggacgccccc gggagctcag atacgcgctt ctccgcctcg ggcggaatgg gcctgcgctg
     6481 gccagtgaac cgacatgttg ccttgcgcgt ggaaggtcgt ggtctggcca gcctgatcaa
     6541 tgccgacacc gcgatcttct gtcgctccga tcaaagcggg ctgttgtgcc gtattcatgg
     6601 ccagggccga acctttctcc aggggatgct cctggcggga gtggcagtga atttctgagg
     6661 accgcagctc aggcgagctg cacgtagctc aggggcaagg cggtcgtgta tttgatcgtc
     6721 tcgagcgcga agctggcgct gacgtcctgg aattccactg cggcgatcag cctcttgtag
     6781 accacgtcat aggcatcgat gtccggaacc acgatgcgca tcaggtagtc cacatcgccg
     6841 ctcatgcggt ggatctccgt gacctccgga atgtcgcgca cggcgcgcac gaatctgtcg
     6901 aaccacgcct cgctgtgatt gcccgtcttg agggtaacaa aaacggtggt gccgacgttc
     6961 agcgcccggg catcgagcag cgcgacggtc tgcttgatga tgcctgcctc ttccaggcgc
     7021 ttgagccggc gccagcaggg cgccttcgag aggccgacgc ggtcggcgag ctcggcggca
     7081 gtcagccccg cgtctttctg gatcaggtcc agaatcttcc ggtccagctt gtccacgata
     7141 tttcgtctcc ctgtatcgat gatacgagaa atattgttgc gcagtcaacc ctggatcagt
     7201 ccgtcttcgc aaccctgtag cgccccgggc gagctaggct gtaggcatgt cattagggcg
     7261 cctgttcacg gaacatccgg cttcggtggg cgagagctat atcgctcatc tgctgagagc
     7321 tgcctggttc ggcagccgta tgatgcttgc cggcggcgcc tgtctcgtgc acgccatttt
     7381 cccgtttctg tttgtcaaaa caggcagtaa cgccatcgca gagctgcata cggcgatggt
     7441 gacagggcgg cggcccacgg cgggcccgac tctcgagagc cggcgcgttc cgagctagaa
     7501 gcccttcaca cgggccgcgg cattaggggg cggtcaggcg ctctcggcgc tgctgtgcac
     7561 caggtaaccg cctgacttct catcccgctc gagcgtgatc agccgccgct gtgcggcttc
     7621 ctccagcagc ttgttgaaag agcggaatcc gtagtagctc tcattgaagc ccggtcgctg
     7681 acgcttcaga gcctgcttca ccatcgagcc ccagatcttg tcctcggccc cgcgctcgga
     7741 cacgagggca tcgagcgtcg ccatcacgag atcgaatgct tcctgcttgt gatcctcttc
     7801 gcgatgctcg gactcgccac gcgactcgcc gccacgtccc ggctcggccg tctgcgccga
     7861 cggctcgctc gccggggcct tcttgcgcgt gcgccgcgcg cgcggctgct ctctgaccag
     7921 atcatcgtag tagatgaact cgtcgcaatt cgcgatgagc agatccgaag tcgaattctt
     7981 cacgcctacg ccgatgacgt ctttgttgtt ctcgcgcagc ttgctcacga gcggcgaaaa
     8041 atccgaatcg ccgctgatga tcacgaagct gtcgacatgc gtcttggtgt agcagagatc
     8101 cagcgcatcg accaccatgc ggatgtcggc ggagttcttg ccggactgct tgacgtgcgg
     8161 gatctcgatc agctcgaagc ccgcgagatg catgccggac ttgaactcct tgtagcgttc
     8221 ccagtcacaa taagccttct tgacgacgat cgagcccttg aggagcaacc gctcgagtac
     8281 gcgattgatg tcgaattgcg cgtaacgcgc atcgcgcacg ccgagggcga tgttctcgaa
     8341 gtcgcagaag agcgctagat tggtggtgtc ggcccctgag ggcatggcta tcgtccgtcg
     8401 cagtgagcgg cccgcagcgg cggattctga caggactgcg agcaaagcgt ggagtttttc
     8461 ggggcgctgg cgccggcctc gagcgcctgc gccctgtagg ctatagtccg tggagcaggc
     8521 gccgcatctg gccgaaagat ggctcgctca tggcatctga aaacattgcg gcgatcttcg
     8581 gcaaggtcct gcgggaactg cgcaacgcgc gcggcatttc gcaggaggcg ctggccctga
     8641 gtgccgacgt ggaccgcact ttcgtcagcc agatggagcg cggaatccgt cagcccacgc
     8701 tcacgactct gtgcaagctg tccggagcgc tcgagatcca gccctcgacg ctggtagcga
     8761 aggtcgagcg cttgatgcga taggctgagg gccgtaccca cgccgcttcg tacgcgcaag
     8821 tcttgcccac tttcccgggc atcgctacgc tgcgcactgc tggacccccc gaaggcaccg
     8881 aacgacaaga gggatggatg gcagacaagc agcggcgaaa agtctcgcgc aaggcggcag
     8941 aggaggcagt gcgcacgttg ctgcgctggg ccggcgatga ccccgaacgc gagggtctgg
     9001 ccgatacgcc caaacgggtc gccaatgcct atcgcgactg gttctccggc tacgacatcg
     9061 atccggccga gtacctgcgg cgtaccttcg aggaagtggg cggctacgac gagatgatcg
     9121 tgctgcgcga catctcattc gagtcgcact gcgagcacca tatggcgccc atcatcggca
     9181 gagttcatgt gggctatctg ccgaccaaca aagtagtggg catcagcaag attgcgcggg
     9241 tggtcgaagg ctacgcgcgg cgtctgcagg tgcaggagaa gctcaccgct cagatcgcaa
     9301 actgtatcaa ctccgtcctc aagcctcgtg gcgtgggcgt cgtgatcgac gccacgcacg
     9361 agtgcatgac gacccgcgga atacacaagc gcggcgtctc gatggtcacc agccgcatga
     9421 ccggcacttt ccgcgaggat gcacgcacgc gaatggagtt tctgcagttc gtcgacatca
     9481 gcggcgtcag agaggtcagc tagcgcctgt tcagacttgc ggtggctccg gcacaggagc
     9541 gcctctttcg catccagtgc cggggcattc gagccgctac tgagtcttga gctcccgctt
     9601 cttgaaatcc gccgggacct cgaaggcatt gggggtcgca acatccgtgg ttacagagac
     9661 gagctgcgtc gagaccgtca tcatgacgac gtcgccggcc ggcaccggcg gggcatctgc
     9721 tggctgagcc ggctgagccg gttgcgcggc ctgctcgtcg tccttcttct tgtggaacag
     9781 tgagccgagc ttgccggcga cctggcctgc caggttggcc gtgccgccgc cacctccact
     9841 gctattgccc gaggactcct cgctcgactt cgcctgctgt gcgttcttgc actgctcccc
     9901 gccgagcgcg agcgtgaagc tcgatttgac cggatagccc ttcatgccct gcatcttcga
     9961 gacgagctcg ctccagatgc ccttgtagcg gccgaacatg gcctgcgcgc gctgggagaa
    10021 gtcctgcgag gaggacacat cgaagcccag cttggcggcg taggccttgt gatatttctg
    10081 cgcttcggag ttttccgtga agccgcttgc cagccactca tcgagcgcga gcgtgatctc
    10141 gcagatggcc ccggtctcct tgtccttgca tggctgcgag gcgagcagcg tgagccgctc
    10201 cgcatcgtaa ccggcgatct gggccttctc gccggtcttc ttcacatcga ggcggggagg
    10261 cagccactcg cacttcgatt catcgaccgc cgagggtccc tgctgctcct ccttctcctt
    10321 ctggcccgcc tgattcatct ggtcagcgag cttgttcatc tgctcgcgca tctgctcgaa
    10381 ggtcgtctcc gtgtattcct tcttgttgat gttgagatga tagatccggt cgtcgccgag
    10441 gcggacgatc gtggcagagg gcccgacggc gttgtgcgcg agcagacgca ccagacgcga
    10501 ctgcatctgg atgtcgctgt ccatgcgcga tttgtcaccg gagatcgtgg tgcgcgtcgt
    10561 gcccttgagg ttgccaaacg ccatcgcgcc tatgccttcc acggcgatcg tgttgaccac
    10621 ggtgacatcg gcccgggcgg ttcctgcggc gagagtgagc agcccggata gcgcgcagag
    10681 cgcggccggg cgcgagagcc tgctgatcat gcgttccccg aaagtctggt tgtacttgcc
    10741 tgcgcccagg gcgcttctgg gggctgatgc taccaagccg tgccgcggct gtgtaacagg
    10801 ctgattctca aggagcttgc tccggcaaag ggccgcgcgg cgggtctcgg gtcgagctgg
    10861 gcgccaggcg ccctggggtg agcggtgtaa agccggatcg cgttcagacc gtcagcgccg
    10921 gctcgctccg tgccgcgcgt ttgcgctcct gctcgcgcag gtatctcttg cggatgcgga
    10981 tcgagatcgg cgtgacttcc acgagctcat cgtcggcgat gaactccacc gcatactcca
    11041 gagtaagctc gatgggcgga gtcagaagga tcgcctcatc cttgccggcg gcgcggatgt
    11101 tggtgagctt cttggtcttg atcgggttca ccaccaggtc gttctcgcga ctgtgaatgc
    11161 cgatgatcat cccttcgtaa agcttctcgc cgggactgac gaacagtctg ccgcgctcct
    11221 gtagattgaa cagcgcgtag ccgacggcct cgcccgcctc gttgctgacc agcaccccat
    11281 tgtggcggtc cgggatttca cccttcaccg gcgcatagcc atcgaagatg tggctcatca
    11341 gcccggtacc gcgcgtgagc gtcatgaatt cgccctggaa accgatcagc ccccgcgccg
    11401 gcacgcggta ttccagccgc gcgcggccgt tgctgccgtc gatcaccatg tccgtgaggt
    11461 cggcccggcg cacgccgagg gcttccatga ccgcaccctg atggctctcc tcgatgtcca
    11521 ccgtgagcgc ttcgtagggc tcgtgtacct cagtctcgac gacccgggtc agcacctgcg
    11581 gacgtgagac cgcgagctca tagccttcgc ggcgcatgtt ctcgatgagg atcgtgaggt
    11641 gcagctcacc gcggccggag acgcgcatga cgtcgggctc atcggtgtct tccacccgca
    11701 aggccacatt actttgcagc tcacgataca ggcgctcacg cagctgtcgg ctggtgacgt
    11761 acttgccctc gcggccagcc agtggcgaag tattgacctg gaagttcatt gcgagcgtgg
    11821 gctcgtcgac ccggatgggg ggcagcccct cggcggactc gggcgcgcag acggtgagtc
    11881 caatattcac ggcctcgatg ccggtgacgg ccacgatgtc acccgcagtc gcctcgtcca
    11941 ccggcgcgcg ctgcaggcct ttgaaggtca gcacctggcc gatcttcgcg gtaccgcgat
    12001 cctcctctcc gaaccgaaga gccacgacct gtcctgggcg caccgcgccg cgacggatcc
    12061 ggccgatgcc gatgcggccg acatacgagt tgtaatcgag cgtggagatc tgcagctgca
    12121 gaggcttggc ggcctcggcc gccggcggcg gcacgtgtgc caggatcgct tcgaacagcg
    12181 ccgccatgtc agggcgccgc tccgtgtagt cggtgctggc ccacccctga agggcagaag
    12241 cgtagatgac cggaaagtcg agctgctcgt cgctcgcgcc cagcttgtcg aacagctcga
    12301 aggtctgatc gatgacccat gcgggacgcg ccccgggtcg gtcgatcttg ttcactacca
    12361 cgatcgcctt gagcccgagc gcgagcgcct tgcgtgtgac gaagcgcgtc tgcggcatgg
    12421 gaccctcgac ggcgtcaacc agcagcagca cgccgtccac catcgacaga acacgctcga
    12481 cctcgccacc aaaatcggcg tgccccgggg tatcgacgat attgatccgt gtgcccccgt
    12541 attcgatcgc gcagttcttc gccaggatcg tgatcccgcg ctcgcgctcg atgtcgttgg
    12601 aatccatgat ccgctcgccg agcttctcgt gcgcggcgaa ggtgcccgac tgcttcagca
    12661 gacagtcgac cagggtcgtc ttgccgtggt cgacgtgggc aatgatggcg atgttgcgga
    12721 tcgggcgatt catgggggga ccaaaaaagg acgcgcaggg taacagattc cgcccttcgc
    12781 tgccggactt tgccgggctg caagtggttg aattcgaagg gaggttaggc tcccgggccg
    12841 tcttgagcgc cgcgggcgcg accgcggcgg cacccacggg ggctctcgca ggccgagcct
    12901 acgcacgggc ggcctcgccg ggtgagctgc cgcgcttcag gttccctggc gcttctgcgg
    12961 cccgatgcgc aaatgcgagg tcgcgattcg aacctcgacc aggaacgcga ggtatgcccc
    13021 ggtgatgaag atcatggacc ccacgaacag caacgcaatg gtggcagcga accccgagcc
    13081 gaaaaacgca ttcgcgaaca gcgtgatgac gaccagagca accagcacgg ccgagcagcc
    13141 acacatcgcg atcgcggcat agatgtatcg agctcgcttc gccagcacgc gcaggctcgc
    13201 ctcaatctcc cgtgtctcgg ccggacgctc gagcagtttg ccctcgagcg agcgcgctcg
    13261 gtcgacgaca cgtgtcagtc tcccggtcat gacgttcagc gtgacaccaa cgcccgacag
    13321 caggaatacg ggggcaagcg ccgattggat caggtgtgcg atgtccatag actcacggtc
    13381 ccggggccga caccgctatg atgccagtca tatgagccct gtcacgagcc gcgcgcatcg
    13441 ccggcgactc ccggcccggc ccacaggcga aaagtccaac acgcggtcgc tgctggcaac
    13501 ctgccgccag gaatccgtgc agctcctgcg ccgcaacctg tctcccggcg ggatcctggc
    13561 tgcaacacca ggtgatcgag cgcgtgcgcg gagctacacg gcgatttatg gacgtgatgc
    13621 ggccgtatgc gccattggca tggccctgtc cggcgatgag atcctgaggg gtgaggcccg
    13681 ggccggtctg cggacgctcg cggagcatca ggccccgaac gggcagatcg caaagttcgt
    13741 ggacttgagc cgacaggaag ccgatttctg gtatctcggc tgcatcgatt cgaccttgtg
    13801 gtggctgatt gcgctcgccg ttctggaccg gttggcccac aaaaccgagc gcagcgggct
    13861 gcgccgccac tatgcccgcc acatcgagcg tgcgacgcag tggctgctgg cgcaggaaca
    13921 tcagcgcttc ttcctgctgc agcagaatga ggcaagtgac tgggccgaca tcatgccgcg
    13981 ctcgggattc gtcctgtaca cgaatgcgct ctggtactgg gtgaagcgct cgtatgggct
    14041 accgcatgct gcggctacct acgggaattt caacgggctg ttccatccct tctcggcagg
    14101 tctcgcgccc tatcggcggg cgcgcctgct caatgactat gtcctgcggc gcggacggga
    14161 tcgctcgttg tacctgagct tcgtcaactt ctcgttcttc ggcgaggagg gcgatgtgtt
    14221 cgggaatgtg ctcgcggtac tgatgggctt ggccgacccg cgggcgatgc gccgaacgct
    14281 gcgcgcgttg caacgggcgc acatcggcgc tccctatccg gtccgagcag tcatacagcc
    14341 gatccgacag agcagtccac tgtggcgtcc ttacatggtc cggcatcggc agaacctgat
    14401 gtggcagtac cacaatggcg gcatctggcc catggtcggc ggtttctgga tcgcagcact
    14461 cgcagcggcg ggccagctcg gccacgcccg caccgagctc ctcgggctag cgcgcgcctg
    14521 cagcatcaat gattggggct tcaccgagtg gctgcatggg cgcacgcttg cgccgcgtgg
    14581 gatgccgggt cagtcctgga acgccgccgc gtttctgatc gctcagcacg cggtggagac
    14641 ggggaagaat gtgtttgatt taggccccag gcgctccgtc ctgcgcgaac gactggattg
    14701 agaatccgcg ggccgtcatt cgattccgcc cgccgcctgt ccaatactcg gctcgctgtc
    14761 tagtattttc gcgccaggat gcggccgccg tggtttgcgc aggtagacct ttcgggtctc
    14821 gtgcgcaata caggatctgc gccatcccgc tggcggcaca ctcgagcgcg cacccgacgc
    14881 gcagcgtgcc gaaccgagtt cgacagccga aaccgtgtct aacaacaaag cccaaccgaa
    14941 gtaattcggt tcagctctta tccagcgtgt tcaacagcgg cttgaatccg ccccagaaca
    15001 tccgcttgcc atcgaacggc atgggctgca cccccggttg catttcggga tcggccatca
    15061 gtttcttcca cgccgcatcg cggacgtcct tcgacggcca gaggataaag gagaagacga
    15121 cattctcgtt gtcctgcgcc tttacagcct tgaagaagtc cgttgtgtga ccgtgctcga
    15181 gcccgtcgcc gagcgcctcg attgtgccga tggcgccttc cgcctgcgct ttcttggcga
    15241 aggtctcggc catttggcga tattcgcctt ctttcccgtt cgggagcggg acaacaaatc
    15301 cgtcaacata ggccatagcg ggtctcctca gtgtagcgat gagtttccct cggcgatcgc
    15361 gctggaagtg gtgccgacgc tgcgctcagc cggcgcaggt ccatccacgg gatccgctct
    15421 gaattctgcc cctccgcgtc ctatttggcg agcggtgcgg ttcccgcatg gccgggcagg
    15481 gcgtgcggga tttggcgacc tcgtcattcg ggtccagctc gcggacgggc gcgccggcgc
    15541 gggcggtcaa gctcaaggaa agcgtactgc tggcggaacg cgggccgtcg atcattagca
    15601 tacgcgtcgg ctcgcgagcc cccgccccaa caccgtgtcg aacaagaggc tgccgatgga
    15661 cttgacctgc tgcgtgggcg cagcgccgcg aatgagcgtg agcacgccat ttcatttcaa
    15721 aatgacttcg cgaacgtatt caatgcctac cactgttctg tactgattcc ggcccaccct
    15781 ggccttgttc tgaacctcgg tgctcccgat caccttgttg gccacgtctt ccagctcgac
    15841 ctctttgtcc aaagcagccg ccatgttctt aaaagtgatc atgagaaata tatttggctg
    15901 atccggtgct tttggagtgg cccggacgac tttgtaatcg aggatcagcc ccgctttctt
    15961 tgtggcttcc atggtcggct tccaggtcga attgagccaa tcgatgtact cgtcaaaatg
    16021 accgtactca actcgtatgt agtccatctc agtgaccggg ccatccgtat actggcgcac
    16081 atcctgcgcg cgtgcactga gcgcaagtga ggaagctgcg agacacatgc ctaagaagat
    16141 ggttgtccta atcattttcg cttcaccttt tgtgagagat ctctactcgg ctaacatccg
    16201 aatcaacctc ggtccgctgt ctcgaacagc cttgaggcaa tctcgggcgc gtgggaggtc
    16261 aggacaccca cccgtgccag ggcatagatt tcggtcgcgc atcacggcgg tccaaccacc
    16321 atagaaatgc cacaggtccg ccgagagctc gcgtccaacc gatcgagctt cgatgagcct
    16381 gccatcagat ccgcgcaagt ccatgccggc cgcactggcc gcgatgttgt accggatggg
    16441 aggcgatgcg aactcgcaag tcgccataaa acgatcaaga agaagggtgg aaggtccatc
    16501 gctttcaccc ccgatcaggg accaggtggg tccaccaaca ttgctcagcg cctgccatgc
    16561 gctccgttgc ccgatcggtg tcaggctgcg gatcgcaccg atttccaccc aaacgtgttc
    16621 cggctgatag tctgctgcaa tacggaacat tctactactt cattatcttc acgattatag
    16681 taccctggtc gcacgattca gcgcttcgcc ggaggagcgc tttgggagat acccttgctc
    16741 gatgatctac gcgccctcgt agagttcgcc cacgctgggt ccatcgccgg tgcagccgat
    16801 cgtctatttc ggacgccctc cgctattacc cggcaagtgc aaaggctgga ggctgcattg
    16861 ggtgccgaat tgctcgaccg ctcggtaaag cctgcgcgcc tgaactcgct aggcgcccga
    16921 gtgctcgaac aagcacgaga tctgctgcag cggaccgagg cgctgaaatc tctcacggcg
    16981 agcgacgccg aacctcacgg cgtcttgcgg atcgggctct cgcacccctt ggccgagagg
    17041 acgctcatcg atcctattct ggcgctgact gacaaatacc cgaaggtccg actgcggctg
    17101 gtgagcgaac tgaccgttga gttatatcgc cggctgctcg cgggggagct cgacgtggct
    17161 gtcgtacttt tgcctgaggg taaagccgcg cctccacctc ttctgacgaa tatcatcgcc
    17221 accgaccgca tggagatcgt tcaaggcgcc gcaggcgact tggacggtga ttggaaaagt
    17281 ctcggcgggg ccccatgggt gctcaatcca cccgggtgcc cgcttcgggc cgcactcctc
    17341 gatcggatgg agcgtgatgg ctgctccccc atgattgcgg ctgaagtcca caacatgcat
    17401 ttgcagctgg cgttcgttca ggccggttat ggtgtcgggc tgctgcctgc tcgcttcatc
    17461 gcgaggaacc gctctatcga caccgtgaag ttattgcgcc ctccctcgtt tgacctgcgc
    17521 ccgtcggtcg cgatagtgcg ggcggggcaa ctgggtgcct tggggaaggc cgtcgaattg
    17581 ttggaacacg ggcttcggca attgcttgag gcagccgaca cggcgaagcc cctgccgcgg
    17641 caccgcagag gtgtgcccgc gaatcggcgg gcggcgcgcc ggcccgcgta gtcactgccc
    17701 ttcagtcaaa gccgaacagg gccggcgcac gttagcgagg gcgtctgctt cgccggcgag
    17761 catggcgacg agttggcctg cctcggcggg cttgcgtgaa ccggagaccg gcgcttcgac
    17821 gtagcgtctc ccgacggagc gaatatctgc ctcgagctcg cgcgaatagg ccggcgaggt
    17881 ggtacccata tggacgaccg tgcgtcccgc cacccgcggc gcgaaggcag caccacaacg
    17941 gtcgagtacc gcgtcaatcg cttcgccgtc gctcagcatt aggaagacag tcgcgcagtg
    18001 cgcgaacacg tcagccggct ctcgagccac atcggcgccg gacgcggcag cccgatgaaa
    18061 cggatcggat atgctgcatc cattcggtgc cgcctcgctg ctcctgggtc tgcctccctt
    18121 acggcagata tttcttgggc agcggcgccg aggccgactc cgcctgcacc atgatgctcg
    18181 tcactcgcca gccgccggct tccttgatcg cctggatgct gttgatgccg cacgcgaccg
    18241 gcttgccgtc gctgtgcagc ccgtagctgc tccataaatg cgcaatgttg ccgtagcgct
    18301 cgatgtgaaa tttcaactgc ttttcttcaa gcaggccgtg gccacgggct ttgacacggg
    18361 tgatccaatc gtccaaggtc tggagcgcat aggttggcgc tccgtccgtg ccgcgtgaaa
    18421 cgaacatcac gcgcgcttcg gatatcaaga ggaccttcat gcaggcccga tccttgtccg
    18481 cgggcccgga gattgccgca tcgagcgcgg cgggaatgcc ctcgggttcc gagggcaacg
    18541 cacagtccgc atccggcgcc tgcgccatcg ccttttcgac gagcgcatcg gccagcgttg
    18601 ccgtcatcgc gagcacagtc aagccggcga cgctcgcgat cactctacac acggatctat
    18661 tcatactgtc tcttctctac tgcatgaggt cgaaggagga ggccgcgctg ctcagtgctt
    18721 cggtttgcgg aacttcagga ggaactggtc ggtgtgtccg cgaatcgtcg gatcgaacac
    18781 tttggcggta cggggatcag ccggattggc cagaatcgaa ctctcggagt cgaactcgaa
    18841 tcccgcggcc tcgacctctc ggcgcacggt cgaggcctcg atgcggtgca aggtctcggt
    18901 tacgtttgcc ggagcaccct gcgcggcgga gtgatcgaga atgacatata agccaccggg
    18961 cttcagagcg ccgtagaccg ccttattaaa cgcggcgatg tcgagcgggc ccatgaactc
    19021 gtcgtggagg tcgtggtaat tttgggaaat ccacaacagg tcgagcttct cggggaactt
    19081 cagtccttcg agggccgcgg caatcgtcac cgtcacgttg ggatgcgtga cgggatagct
    19141 ctgcagatcc gtgacgccct taacgaaatt cgggtacttg aagaagccgg tgggttgcac
    19201 cgcataaacg tgaccgttgg agccgacgac ggttgcgaac agacgtgtga aatagcctgt
    19261 gtcggcgaca tagtcggcaa ccttgtcccc cgatttagcg cccgcgaact ctaaagtctg
    19321 caccggcttt cggttagcat cccgtgaggt gtcagagtgg ggacgcgacg gatcggcgac
    19381 tgccgtggtg gcggcccttg aattgcctgc gttgtccatg gcaatcgccg cgtaagcaag
    19441 cgcgacagcc gcgatcaaca tagcgattgt tttcatgtcg actcctatcg tgaatgcttg
    19501 gccatgggct aatacttcaa ctgaaatccg ccgccacatc agccatcagc cctgcagcga
    19561 tacggtctaa gcaaatgggc gatctagacg atttaaggca caagcgcgga gggcggcacc
    19621 tcgatttcgc cgcttgcgat gtaggctgtt gcgcctccaa cgctgacaga ggtcacgaca
    19681 cttccgctct tgcgtgcctc ggcttcgatt tcgctccgac gtcccatcga gacaccttgc
    19741 tggatggaaa ggcgataggt gcgcccgcca aaaccgggcc tcgatgccat tgccccgacg
    19801 agggcggcac aagcgctacc cgtcgcggga tcttcttcga cgccgagcgc gggcgcccac
    19861 atgcgagcat agagcttgcc gccatcccgt agatctcctg agaaaaagaa cacctgggga
    19921 gaccacgcgc gcgcgagcgt ggccgcccat gccacccgat tgatcaccgc ccgatcgacg
    19981 acctcgttcg agctcagttg cgcaaaacag aatcgaacac cgacgcccgc gaagaagact
    20041 tggcgcacct cggcgggctc gatggatagg actgcggcaa gatcagtcgg agaaggtgcg
    20101 cccgtggggg tttcgatctt cgccgataag gtcagcgtgc catgcagact cccctttcgc
    20161 tgagcgacgt cgaccgtcac ggggccgatg ttctcctcca ggatgagacg gattggatca
    20221 ctcagccgtt cgttctcctt catcagaagc acgcaggccg taccgaccgt cggatgcccc
    20281 gcgaagtcga gctccgcccg aggtgagaag atgcgcaccc gacaggtgtt cgccggatcg
    20341 ttcttcggca gaacgaaggt cgtttcgccg aaattgaact cgcgggcaat cttctgcatg
    20401 ccctcagtcg aaatgccggc tgcctcatgg agcacagcaa gttgattacc gccgaatggc
    20461 gtcgaactaa acacatcgac gatgtgaaat gcgtatttca tcaggtcaat ctcccgtcac
    20521 acctcaagcc ttgacgacga ggtcgatctc aacgcaggcc ccaagtggca gcgctgcgac
    20581 cgcaatcgtc gtgcgggcag gaaagggctg atcgaagtac tttgcgtaga tgccattcat
    20641 cgcggcgaag tcgctcatgc gcgtgagata cacagcggcg cgcgcgacgt ggtcgaagct
    20701 tctgccagcc gcctggagaa ccgctgcgag attccgcaag acgcgctctg tctcggcctc
    20761 aatgcctccc tcgaccacct tgccagtggc cgggtcctgg ccgacctgac cgctgacgta
    20821 gaggaaacca ccgacttcga tcgcctggga aaacgggccg gccggtggcg cgagctcacg
    20881 cgaggtcact gcggtcttgc tcatggcaaa ctcctgtgga ctgcggtgat agacgttaag
    20941 gaagttgagg cctgccgccg cctggcctgt gtatctggca cgaccgggct caccgacatc
    21001 gaccgcggtc ccgcttagaa catggggacc gcccatccgc cgagttggga tcgcgaggca
    21061 tgccgattcc cgc
//
LOCUS       DASRHN010000030         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1492223, whole genome shotgun sequence.
ACCESSION   DASRHN010000030 DASRHN010000000
VERSION     DASRHN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492223"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFB37_01140"
     CDS             complement(<1..289)
                     /locus_tag="VFB37_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21075.1"
                     /translation="MNAEPLTDHITQMQTLLERASLLLEGAADIVRGSAPAAACQAIE
                     SAKKNLLELCDINGASPPHETYRLYSVLCVAEHLLRKGIEISGGGVTDNPLA"
     gene            complement(286..480)
                     /locus_tag="VFB37_01145"
     CDS             complement(286..480)
                     /locus_tag="VFB37_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21076.1"
                     /translation="MNEVTTGSVGALSVSELQQAAGFLHAATRLVERLDARQRAPSIY
                     PPRAGLRLVIDNTTNNGGPP"
     gene            complement(537..896)
                     /locus_tag="VFB37_01150"
     CDS             complement(537..896)
                     /locus_tag="VFB37_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21077.1"
                     /translation="MSGPNAMSRLWCSLYDASSFIASKVQDLHPLIGEPMVEAQTAEE
                     LRQIQALVEATLTLLCRVTTRHDLRRKPRERRPQRKRRKPRPPLVLRPSEPVSAVRRI
                     EHLLQRLTALPGSGDAP"
     gene            complement(893..1114)
                     /locus_tag="VFB37_01155"
     CDS             complement(893..1114)
                     /locus_tag="VFB37_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21078.1"
                     /translation="MALGKTYAQRLGLLGQQFSLSHSGVKRALRALPQLCTGPLLPML
                     QALVEGRCASLVIEPAQARELLALLAVQS"
     gene            complement(1681..2394)
                     /locus_tag="VFB37_01160"
     CDS             complement(1681..2394)
                     /locus_tag="VFB37_01160"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HZO21079.1"
                     /translation="MRPIALGLGERALRRALVLACVTAAIFVLAIGVASANPRQHEGP
                     RPGFDHMDARFHHDHYYPSRGYAVHDLPRDRIVVNGPRGRYYFSGGVWYEARGPRFVV
                     VSAPIGLFVPVLPPFYTTVWFAGVPYYYADDTYYAWRPSSGEYEVVDPPGGDPTTDAP
                     PAPPSPNGDVFVYPKNGQSEEDQARDKYECHKWAQGQSGFDPTAANGGVPPEQANARR
                     ADYQRAIGACLEGRGYTVK"
     gene            complement(2410..2997)
                     /locus_tag="VFB37_01165"
     CDS             complement(2410..2997)
                     /locus_tag="VFB37_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21080.1"
                     /translation="MINETSPVPHFKTAARMLVLSCAVAVLAGCVEQPPPHRVYRQPP
                     PPPQANSEVYAYPLQGQTPEQTDRDRYDCHEWAVKQTNFDPSAPGTPPHDRVMVASGP
                     PPGTNTTIGAVAGAVLGAVIAGPRNAGFGAVAGGVTGAAIGSTGDAANAEAQRAQVQQ
                     ARAMDAREAAVMDQKAANYRRAISACLEGRGYSVK"
     gene            complement(3194..3949)
                     /gene="kdsB"
                     /locus_tag="VFB37_01170"
     CDS             complement(3194..3949)
                     /gene="kdsB"
                     /locus_tag="VFB37_01170"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF009905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZO21081.1"
                     /translation="MFRVVIPARYGSSRLPGKVLVGIAGRPMIQWVYERACASTAQEV
                     IIATDDLRVIQAAQLFGADATMTAATHASGTDRIAEVARARAWSDEDIIVNLQGDEPL
                     MPPALIDQVAGVLESAPQAGIATLAAPVESLAELMDPNAVKVVMDARGRALYFSRAPI
                     PWNRDGAPSGPASQSVFAGAWRHVGLYAYRVGALLRLAALGTGVLEAREKLEQLRALE
                     QGLEIRVSGALQRPGPDVNAAADLERVSALLRR"
     gene            complement(3962..>4133)
                     /locus_tag="VFB37_01175"
     CDS             complement(3962..>4133)
                     /locus_tag="VFB37_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZO21082.1"
                     /translation="EILACPICKGPLKYQRDAQVLVCRMDRLAYPIRDEVPVMLEEEA
                     RQLTADDPLLER"
BASE COUNT          694 a         1363 c         1377 g          699 t
ORIGIN      
        1 cggccagggg gttatcggtc accccgccgc cgctgatctc gattcccttg cgcagcagat
       61 gctcagcgac acataggacg ctgtagaggc gataggtttc atgcggtggg ctcgcgccgt
      121 tgatgtcgca cagctcgagg agattcttct tcgcgctctc gattgcctgg caggccgccg
      181 ccggcgccga gccccggacg atatcagccg cgccctccag caagaggctg gcgcgctcca
      241 gcagcgtctg catctgagtg atatggtcgg tgaggggttc tgcattcatg gtggacctcc
      301 gttattcgtg gtgttgtcga tcacgagccg caggccggcc cgcggcgggt agatgctcgg
      361 ggcacgctgg cgcgcatcga gtcgctccac gagccgcgtg gccgcgtgca ggaaaccggc
      421 cgcctgctgc agctcgctca ccgagagcgc gcccactgag ccagttgtca cctcattcaa
      481 tgcagcgacc agatcccggt gccgctcgca gagcctgcgc caagcctcct gagcggtcat
      541 ggcgcatcac ccgagccggg tagcgctgtg agtcgctgca ggaggtgctc gatgcgcctg
      601 acggccgata ccggctcgct gggcctcagg acaaggggtg ggcgcggctt tcggcgcttg
      661 cgctgtgggc gccgctcccg cggcttgcgc cgcaggtcgt ggcgcgtggt gactcggcag
      721 agcagcgtga gtgtcgcctc cactaaagcc tgaatctggc gcagctcctc ggccgtctga
      781 gcctcgacca tcggctcgcc gatgagcggg tgcaagtcct gcaccttgct cgcgataaag
      841 ctgctggcgt cgtacaggct gcaccacagt cggctcatgg cgttggggcc gctcatgatt
      901 gcaccgccag gagcgcgagc agctcacgcg cctgcgcggg ctcgatcacg aggctcgcgc
      961 aacggccctc cacgagggcc tgcagcatcg gcagtagcgg gcccgtgcag agctgcggga
     1021 gcgcgcgcag agcgcgcttc acgccgctat gtgacaaaga gaattgctga cctaacagcc
     1081 cgagccgttg agcgtaggtc ttccccagcg ccatcagggc agtgttggct gcacggatct
     1141 ccgcgtcgcg tttcgcagag cgtgcccgca gcttcgcgag ctgttcaggg ctgcgcggcc
     1201 ctcggcgccc atcaggggtc gcgcgtggag gccgagcggg ggcgatgtgc aggcgcctgg
     1261 tcgcttgctc ggctcgggcg aactgggcga tacgctcctc gacatacggg ctgcggttcg
     1321 gagcacgcgc ctgagcggct gggagcggcc gtggacgcgg tagctgagct ggggggcgca
     1381 gcaggagcag agataggggg gcggagacgg tagaattcgt cgtagccatg ttcagcacct
     1441 cggatcgctg gatgtggtga gccggccgct cgtgggtcaa gcacgggcgg gccggcgctc
     1501 ttgcgcatca gagggaatgc agtcgcacgg tggctgccgg tgtatcccgg cttgtatccg
     1561 gagtgaccta agtcactgag agacaataga aaacctggtg tttcccaggc agacgaacag
     1621 gatcctcatg gaagcggact cgtccgcaaa ctcgggccga cgtccccgcg gcgcaagctt
     1681 ttacttgacc gtgtagccgc gcccttccag acacgcgccg atggcgcgct ggtagtcggc
     1741 gcgtcgggca ttcgcctgct cgggcgggac accgccgttg gcagcggttg gatcgaagcc
     1801 tgactggccc tgcgcccact tgtgacattc atacttgtca cgtgcctggt cctcctcgga
     1861 ctggccgttc ttcgggtaca cgaagacatc gccattgggg ctgggcggag cgggtggcgc
     1921 atcggtcgtc ggatcaccgc ccggggggtc caccacttcg tactcacccg agctcggccg
     1981 ccaggcgtag tacgtgtcat cggcataata gtaagggaca cctgcaaacc agactgtcgt
     2041 gtagaacggc ggcagaaccg ggacgaagag accgatcggt gcgctcacga ccacgaagcg
     2101 cgggccacgg gcctcatacc acacaccacc cgaaaaatag tatcgcccac gcggaccgtt
     2161 gacgacgatg cgatcgcggg gcagatcgtg aaccgcgtag cctcgcgagg gatagtaatg
     2221 atcgtgatgg aaccttgcgt ccatgtgatc aaagcccggg cgcggtccct catgctggcg
     2281 gggattggcg ctcgccacgc cgatcgcaag cacgaatatc gcggcggtga cacatgcgag
     2341 cacgagcgcg cgccggagtg cccgctcgcc cagacctaac gcaatcggcc tcatagtgag
     2401 tcgcaccctt tacttgacgc tgtagccgcg gccctcgagg cacgcgctga ttgcccgcct
     2461 gtaattcgcg gccttctggt ccatgacggc cgcttcccgc gcatccatcg cgcgcgcctg
     2521 ctggacctgg gctcgctgcg cctcggcatt cgccgcatca ccggtggagc ctatggccgc
     2581 acccgtgacg ccgccggcca cagcaccgaa accggcgttg cgcggacctg cgatgacggc
     2641 acccagaacg gcgcccgcaa cggcaccaat ggtggtgttc gttcccggcg gcggacccga
     2701 tgcgaccatg actcggtcat ggggcggtgt gcccggtgcg ctggggtcga aattcgtctg
     2761 cttgactgcc cactcgtgac aatcgtaccg atccctgtcc gtctgctccg gggtctgccc
     2821 ctgcagcgga taggcataca cctcgctgtt cgcctgcggc ggcggcggtg gctgacggta
     2881 cacccgatgc ggcggcggct gctcgacgca acctgcaagc acggcaacgg cgcacgacag
     2941 cacgagcatc ctggcagccg tcttgaagtg cggaacagga ctggtttcat tgatcatctt
     3001 gaatgcttcc agcaaaaacc gtcgatagca aaacgcgagg tgtaggttgg ggttgacagc
     3061 gccactctcc gccccggagc ataccccggt tggattctcg cacgcccgcc tagttccaca
     3121 gacgctggag gtcacggtcc gccccgcgcc gttggcggtg tgaaaaattg taaaactgtc
     3181 gagcccatcc tggtcaacgc cgcagcaacg cgcttacgcg ctccagatca gccgccgcat
     3241 tgacgtccgg gccgggccgc tggagggcgc cactgacgcg gatctccagc ccctgctcca
     3301 gggccctgag ctgctcgagc ttctcgcgcg cctcgagcac gccagtgccg agcgccgcga
     3361 ggcgcaacaa ggccccgact cgataggcat acagaccgac atggcgccag gcgccagcaa
     3421 acacgctctg gcttgcaggt cccgacgggg caccatcgcg gttccaaggt atgggcgcgc
     3481 ggctgaagta caaggcccgc ccgcgagcat ccatcacgac cttcaccgca ttgggatcca
     3541 tcagctcggc gagcgattcc acgggtgccg ccagcgtcgc gatgccggcc tgcggcgccg
     3601 attccaacac gccggccacc tgatcgatga gcgccggggg catgagcggc tcatccccct
     3661 gcaggttgac gatgatgtcc tcatcggacc aggcgcgcgc ccgagccact tcggcgatgc
     3721 gatctgtgcc ggaggcatgc gtggctgctg tcatcgttgc atcggcgccg aacaattgcg
     3781 cagcctggat cacccgtaga tcgtccgtcg cgatgatcac ttcctgagca gtggaggcgc
     3841 aggcacgctc atatacccat tggatcatcg ggcggccggc gatgccgacc aacaccttgc
     3901 cgggcagccg ggacgagccg taacgggcgg ggatcaccac gcggaacacg cgatgcgggc
     3961 ctcagcgctc gagcagtggg tcgtccgctg tcagttgccg ggcctcctct tccagcatga
     4021 ccggcacttc gtcgcggatc ggataggcca gccgatccat gcggcacacg agcacctggg
     4081 cgtcgcgctg atacttgagg ggtcccttgc agatcggaca ggccaggatt tcc
//
LOCUS       DASRHN010000031         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_10623, whole genome shotgun sequence.
ACCESSION   DASRHN010000031 DASRHN010000000
VERSION     DASRHN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10623"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFB37_01180"
     CDS             complement(<1..461)
                     /locus_tag="VFB37_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21083.1"
                     /translation="MSAFLSTHVRAQGAAALAGDFAGNLGQLSLKLHLHAAPDGTLSG
                     TIDSPKQGAMGLVCSEFKLEGTQLSFQVPSVRGSWKGSIEDGGATLAGTWTQVNSKPL
                     KFVRDTFVPAAKPSPVDGFWLGTLHADTQSLRIQITVKSDQSGHEFCTVDSL"
     gene            complement(520..960)
                     /locus_tag="VFB37_01185"
     CDS             complement(520..960)
                     /locus_tag="VFB37_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZO21084.1"
                     /translation="MSGLVTIGYQGVSLEGFLETLKKAGVTLLLDVRELPMSRRTGFS
                     RTPLSQALGAAGIAYRHVRALGTPKRIRHRLRRDRDMKRFFAEYQQHLATQEALLEDL
                     AQTLSGCVALMCFERDPAVCHRSVVAEDLAKRLGTQTRDLFVED"
     gene            complement(1068..2393)
                     /locus_tag="VFB37_01190"
     CDS             complement(1068..2393)
                     /locus_tag="VFB37_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HZO21085.1"
                     /translation="MNANPALNLAPLLDVQGVCKSFRKPDGDELIVLENVNLSLRPGE
                     IVGLLGRSGSGKSTLLRVVAGLDAPSAGAVGYLGARVSGPASGIAMVFQSFALFPWLT
                     VLENVQLGLEAARVPEGEMRRRSLAAIDLIGLDGFESAYPRELSGGMRQRVGFARALV
                     VHPNILLMDEPFSALDVLTAETLRNDFLDLWCEGQLPIKSVLLVTHNIDEAVQMCDRL
                     LIFSTHPGRVVSEISIDLPKPRRALDPRYIALVDRVYAEMTARPSGEHRPGARMERFP
                     GTGIGTVLTHVSSNLLSGLMEALADEPFRGKADLPEIAETLHMEAGDLLPVAESLQML
                     RFAEIERGDIRLTEAGAQFVRSETDERKRLFSRHLLTYVPLAAHIRRVLDERKGKSAP
                     KSRFFDELEDYMAEEAAEQTIRTVISWGRYAEAFAYDDNQQAFSLENPA"
     gene            complement(2426..4150)
                     /locus_tag="VFB37_01195"
     CDS             complement(2426..4150)
                     /locus_tag="VFB37_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO21086.1"
                     /translation="MRLPELPPLSALRSRFVPGQWDVLAFLLVVVFFIYLAEAAHGLT
                     QSLPRLEATPISLAPSALVGYAARTALRMLIAMVASLVFTFTYATLAAKSHRAEIVLV
                     PLLDFLQSVPILSFSIIIVVFLQLTPGRVAGAEMAAIFLIFTSQAWNMAFSFYHSLRS
                     IPDELVEVARNFQLSPWMRFWRLEVPFAMPALIWNMMMSMSGGWFFVVASEAISVGNT
                     TVTLPGVGSYLWLANEQKNLAAIGWAIAAMFVVILLYDQLLFRPLVASADWFRLEQES
                     GLTPDRPWALTMMRRSRLLRLIAGAFRALLRAGNRRSQPSKEPVARVQRRSRWGDYLW
                     YAVLVIIGLLALWKATLFVLSKVSPEEIGHVIVLGFITLVRVAVLIGLASLIWVPIGV
                     AVGTRPRVARIVQPIAQFLAAFPSILFFPIVVSAVVIFRLTPDIWLSPLMILGTQWYI
                     LFNVIVGASAIAPELKYASENLGVRGWLWWKRVALPAVFPFYVTGAITASGGSWNASI
                     AAEVATWGHEKVVAHGLGAYIAGASEAGDFQRSVLGTVVLSLFVITVNRLFWRPLYAR
                     AERKFRIS"
BASE COUNT          884 a         1462 c         1338 g          687 t
ORIGIN      
        1 agactgtcga cggtgcagaa ctcgtgcccg ctctgatcgc tcttgaccgt gatttgaatg
       61 cgcagcgact gagtgtccgc atgcagcgtg ccgagccaga atccgtccac cggcgatggc
      121 ttggctgcgg gcacaaacgt atcgcggacg aatttgagcg gtttcgagtt gacctgtgtc
      181 caggtgcccg cgagtgtcgc gccgccatcc tcgatcgagc ccttccagct gccacgcact
      241 gacggaacct ggaaagacag ttgcgtccct tcgagcttga actcgctgca aaccaatccc
      301 atcgcgcctt gcttggggct atcgatcgtt ccgctcaacg ttccatcggg agcggcatgg
      361 agatgcagct tcaaggagag ctggccgaga ttgcccgcga agtcccctgc cagggcggct
      421 gcgccctgcg ccctgacatg ggtcgacagg aaagcactca tgagaagcca cccaagcacc
      481 gcgacatacc gggatttccg cgagttcatc tcccgctcct cagtcctcga caaacaggtc
      541 ccgagtctgc gtgcccagcc gcttggccag atcctcagcc accaccgatc gatgacacac
      601 ggccggatca cgctcgaagc acatcagcgc gacacaacca ctcaatgtct gcgcgagatc
      661 ctcgagcaat gcttcctgtg tcgccaggtg ctgctggtat tcggcgaaga agcgcttcat
      721 gtcccggtcc cggcgcaacc gatgccggat acgcttcggc gttccgagcg cccgcacatg
      781 tctgtacgca atacctgccg ccccgagtgc ctgcgacaac ggcgttctgg agaaacccgt
      841 acggcgcgac atgggcagct cgcgcacatc gagcaatagc gtcacgcccg ccttcttcaa
      901 cgtttcgaga aacccctcca ggctcacccc ctgatagccg atcgtgacga gcccagacac
      961 ccgatgcgct tcattcacaa tccgaagtgc ctccgtaggc tcactgtttt cggggagccg
     1021 cctatcggtg gtctaccatc caggctgcgc aaacccgccg acctgactta agccggattc
     1081 tccagactga acgcctgctg gttgtcatca taggcgaacg cctccgcgta ccgcccccag
     1141 ctgatcacgg tccggattgt ctgctccgcc gcctcttccg ccatgtagtc ctcgagctca
     1201 tcgaagaacc ggctcttggg agcggacttg cccttgcgct catcgagcac ccgccgaata
     1261 tgagcggcga gcggtacgta ggtgagcagg tgacgtgaga acagccgctt gcgctcgtcc
     1321 gtctcggagc gcacgaactg cgcccccgcc tcggtcaggc ggatgtcgcc gcgctcgatc
     1381 tcggcgaagc gcagcatctg cagcgactcg gccaccggca gaagatcgcc ggcctccatg
     1441 tgcagcgtct cggcaatctc cggcaggtcc gccttgccgc ggaaaggctc atctgcgagc
     1501 gcctccatga gacctgacag caggttggag gaaacatggg tcagcaccgt gccgatgcct
     1561 gtgcccggaa agcgctccat gcgagccccg ggccgatgct caccgctggg ccgcgcggtc
     1621 atctccgcgt acacgcgatc gaccagcgcg atgtagcgcg ggtcgagggc gcggcgcggc
     1681 ttgggcagat cgatggagat ctcgctgacc acccgccccg gatgagtgga gaagatcagc
     1741 agccgatcgc acatctgcac ggcctcgtcg atgttgtgcg tcacgagcag caccgacttg
     1801 atgggcagct gcccctcgca ccacagatcg aggaagtcat tgcgcaacgt ctcggcagtc
     1861 agcacatcga gcgccgagaa cggctcgtcc atcagcagga tattggggtg cacgaccagc
     1921 gcgcgggcga agccgacccg ctggcgcatg cccccggaga gttcacgcgg ataggcagac
     1981 tcgaatccat ccagcccaat gaggtcgatc gccgcaagcg accgccgccg catctcaccc
     2041 tccggcaccc gcgccgcctc gagcccgagc tgcacgttct cgagcacggt cagccacgga
     2101 aaaagcgcga agctctgaaa gaccatcgcg attccggagg caggccccga tacccgcgcg
     2161 cccaggtaac cgacggcccc ggccgagggc gcatcaagcc cagcgaccac acgcagcagg
     2221 gtggacttgc ccgagccgga gcgtcccagc agccccacga tctcgccggg gcgcagactc
     2281 agattgacgt tctcgagcac gatgagctcg tcgccgtccg gcttgcggaa ggacttgcag
     2341 acaccctgga cgtcgagcag cggcgccagg ttcagtgcgg ggttggcatt cacggtattc
     2401 tctttcgaac tctttcaggc tgctttcaac tgatgcggaa cttgcgctcg gcacgagcat
     2461 ataacggccg ccagaacagc cggttcaccg tgatcacgaa cagactgagc acgaccgtgc
     2521 cgagcacgct gcgctggaag tctcccgctt ccgaagcccc ggcgatgtaa gcgcccagcc
     2581 catgcgcgac gaccttctcg tgcccccagg tggccacctc cgcggcgatg ctcgcattcc
     2641 aggagcctcc cgaggcggtg atggcgcccg tgacataaaa cggaaacacc gcaggcagcg
     2701 ccacccgctt ccaccacagc cagccccgca cgccgagatt ctcgctcgca tacttcagct
     2761 cgggtgcgat ggcggaggcc cccacgatga cattgaacag gatgtaccac tgcgtgccga
     2821 gaatcatcag cgggcttagc cagatgtcag gggtcagcct gaagatgacg accgccgaga
     2881 cgacgatcgg aaagaacagg atcgacggaa aggccgccag aaactgtgcg atcggctgaa
     2941 cgatgcgcgc cacgcgcggg cgggtcccca cggcgacacc aatggggacc cagatgaggc
     3001 tcgccaggcc gatcagcacc gcgactcgaa cgagggtgat gaagcccagg acgatgacat
     3061 gaccgatctc ctcgggtgac accttgctca ggacgaaaag tgtcgctttc cacagggcca
     3121 gcagcccgat gatgaccagg actgcatacc agaggtagtc gccccagcgg gagcgccgtt
     3181 ggaccctcgc cacaggctcc ttcgagggtt gggagcgtcg attacccgcc cgcagcagcg
     3241 ccctgaaagc gcccgcaatc agccgcagca gacgcgagcg gcgcatcatc gtcagcgccc
     3301 agggccgatc gggagtgagc ccggactcct gctcgaggcg gaaccagtcc gcgctggcga
     3361 ccagcgggcg aaacaagagc tggtcgtaca gcaggatcac cacaaacatc gccgcgatgg
     3421 cccatccgat ggccgcgagg ttcttttgct cattcgccag ccaaagatag gacccgacgc
     3481 ccggcaaggt caccgtcgtg ttgccgacgc tgatggcctc cgaggccact acgaagaacc
     3541 agccacccga catcgacatc atcatgttcc agatgagtgc cggcatggcg aaggggacct
     3601 ccaggcgcca gaaccgcatc cacgggctga gctgaaagtt acgcgccacc tcgaccaact
     3661 catccgggat gctgcgcagc gagtgataga agctgaaggc catgttccag gcctgactgg
     3721 tgaagatcag gaagatcgcc gccatctccg cgccggccac tcggccggga gtgagctgca
     3781 ggaagaccac gatgatgatc gagaagctca ggatgggcac cgactgcagg aagtccagca
     3841 gcggcaccag cacgatctcc gcccggtggc tcttggctgc caaggtcgcg taggtgaagg
     3901 tgaatacgag cgaggcgacc atggcgatca gcatgcgcag ggcggtgcgg gcggcgtacc
     3961 ctaccagggc cgagggtgcg agcgagatcg gcgtggcttc caggcgcggc agggactggg
     4021 tgagcccatg tgccgcctcc gcgagataga tgaaaaacac caccaccagc aggaaggcaa
     4081 gcacgtccca ctggcccggg acgaaccggc ttcgcagcgc agagaggggt ggcagctcag
     4141 gcagacgcat atccgcgcaa gtttgcccga attacccgag tcttggcacg ccgccggggc
     4201 ccgcattgca ttcggtccgc ctcatccgac aaggggctac aatgcggttc ccgagccgcc
     4261 gaacgtcaca cgaatgcgtc tgctgatcgt tgaggatgat catgagctgc gcgaggcgtt
     4321 gaccgccatg ctgcagcagt ccggctatga agttgacgcc catgtggatg g
//
LOCUS       DASRHN010000032         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_834813, whole genome shotgun sequence.
ACCESSION   DASRHN010000032 DASRHN010000000
VERSION     DASRHN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834813"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..805)
                     /locus_tag="VFB37_01200"
     CDS             complement(140..805)
                     /locus_tag="VFB37_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZO21087.1"
                     /translation="MAEGGPVKLAVFDLDGTITRHDTLLPFVLGFARHTPWRLAGILL
                     FLPHLALFVLGVSDRGALKSAFIRCTLAGVRRSQIDSWAARFVPTLLERGVFAQALEH
                     IARHKQEGARLALLSASTDLYVPAIGKALGFDEVICTGVRWEGDRLEGHLTTPNRRGP
                     EKARCLESLRNAHPGLVTAAYGNAGSDLEHLQLAERPLLINPSHSARRAAQRLGIPCA
                     SWR"
     gene            complement(821..>2964)
                     /gene="acnA"
                     /locus_tag="VFB37_01205"
     CDS             complement(821..>2964)
                     /gene="acnA"
                     /locus_tag="VFB37_01205"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZO21088.1"
                     /translation="GRVIFDAQVNGTQRAYPDTLVGTDSHTTMINGLGVLGWGVGGIE
                     AEAAMLGQPVTMLIPQVIGFRLNGRLKPGATATDLVLTVTEMLRKKGVVDKFVEFFGD
                     GLANLPLADRATIANMAPEYGSTCGIFPIDEETIRYLELSGRPRERIELVSAYAKAQG
                     LWRNNGDRAADYTDVLELDLGKVEPSLAGPKRPQDRVPLQSARKVYELHGKKSAEERA
                     ARNTAAQGSASVTLEGHSFELKDGAVLIAAITSCTNTSNPSVMIGAGLLARKARERGL
                     MSKPWVKTSLAPGSRVVTDYLQKAGVLGDLAGLGFELVGYGCTTCIGNSGPLKPEISA
                     AVKAGDITACSVLSGNRNFEGRVHPEVRMNFLASPPLVVAYALAGTLDIDLTSEPLGK
                     DKAGKPVRLAEIWPSDQEVQEVLLRSIDSQMFRKSYASVFAGDENWTSIQIPAGKRYT
                     WDTRSTYVKNPPYFEGMTMTPAALADIHGARALAVLGDSVTTDHISPAGNISKSSPAA
                     RYLVEQGVKPVDFNSYGARRGNHEVMMRGTFANIRLRNLLVPGVEGGVTAHVPSGEQM
                     SIYDAAMRYKRDGVPLVVLAGREYGTGSSRDWAAKGTMLLGIRAVIAESFERIHRSNL
                     IGMGVVPLQFHPGQNAQSLGLSGQEVFDITGLGTGEAREVTVRAQAPGKPPIQFTARV
                     RIDTPKEREYYRHGGILQYVLRQLAAPGRAA"
BASE COUNT          491 a          981 c          978 g          514 t
ORIGIN      
        1 acccatattc cgacggtctg acggccgccc ggcgcaccgc cacaacgcgg gcagagccat
       61 ccgagcagac ccgagcacgg ccgcttttat tgcgcacagc acagacttgg catcggtctc
      121 cgccacgccc ctgcgctcct cagcgccagc tcgcgcaggg gatgccgagt cgttgggctg
      181 ccctgcgagc actgtgcgaa ggattgatca aaagaggccg ctcggcgagc tgcagatgct
      241 cgaggtcgct cccggcattg ccgtaggcgg ccgtgaccag gcccggatgg gcgttgcgaa
      301 ggctctcgag gcagcgcgcc ttttcgggcc cgcgccgatt gggtgtcgtg aggtgcccct
      361 cgagccgatc gccttcccag cgcacgccgg tgcagatgac ctcatcgaaa cccagggcct
      421 tgccgatcgc cggcacgtac agatccgtac tcgcgctcaa cagggccagt ctggcgcctt
      481 cctgcttgtg gcgcgcgatg tgttccagcg cctgcgcaaa gaccccgcgc tcgagcagcg
      541 tggggacgaa gcgggcggcc caggaatcga tctgcgagcg gcggacaccg gccagtgtgc
      601 agcggatgaa ggctgatttc agcgctccac gatccgagac tcccagcacg aagagcgcca
      661 gatgcggaag gaagagaagg atgcccgcaa gccgccaggg cgtgtgccgt gcgaacccca
      721 ggacgaaggg caacaacgtg tcgtggcggg tgatcgtgcc atcgaggtcg aacaccgcaa
      781 gctttactgg gcccccctcc gccactgcga gcccgtcagc tcacgccgcc cgtcccggcg
      841 ctgcaagttg gcgcagcacg tactgcagga ttccgccgtg gcggtagtat tcgcgctcct
      901 tcggggtgtc gatccgcact cgtgccgtga actggatcgg cggcttgccg ggcgcctggg
      961 ctcggaccgt cacttcccgc gcctcgcccg tgcccagacc cgtgatgtcg aagacttcct
     1021 gaccgctcag gccgagcgac tgcgcgttct gaccgggatg aaactgcagc ggcacgacgc
     1081 ccattccgat caggttagag cgatggatgc gctcgaagct ttcggcgatg accgcccgga
     1141 tgccgagcag catcgtcccc ttggcggccc agtcgcgtga ggagccggtc ccgtactccc
     1201 ggcccgcgag caccaccagg ggtacgccgt ccctcttgta gcgcatggcg gcatcgtaga
     1261 tggacatctg ctcgccgctc gggacgtgag cggtgacacc cccctcaacg cccggcacga
     1321 ggagattacg caagcggatg ttggcgaacg tgccccgcat catgacttcg tgattgccgc
     1381 ggcgcgcgcc gtaggagttg aaatcgaccg gtttcacgcc ctgctcgacg aggtaacgcg
     1441 ccgcggggct cgacttcgag atgttgccgg ccggggagat gtgatcggtc gttaccgagt
     1501 cacccaggac ggcgagggca cgggcgccat gtatgtccgc gagcgcggcc ggcgtcatgg
     1561 tcatgccttc gaagtacggc gggttcttca cataggtcga gcgggtatcc caggtatagc
     1621 gcttgcccgc gggaatctgg atgctggtcc aattctcatc gccggcgaac acgctggcat
     1681 agcttttccg gaacatctgc gagtcgatgg agcgcagcag gacttcctgc acttcctggt
     1741 cgctgggcca gatctcggcg aggcgcaccg gcttgccggc cttgtccttg ccgagaggct
     1801 cgctggtgag atcgatatcg agcgtaccgg cgagagcgta ggccacgacg aggggcgggg
     1861 aggcgaggaa attcatccgc acttccggat gcacgcgccc ttcgaagttt cgattccccg
     1921 acaggaccga gcaggcggtg atgtccccgg ctttaacggc ggcggagatc tcaggcttga
     1981 gcggcccgga gttgccgatg caggtcgtac agccataacc gaccagctcg aagccgagtc
     2041 ccgccagatc cccgagcacg ccggctttct gcagataatc ggtcacgacg cgcgaacctg
     2101 gcgcgagact cgtcttcacc catggcttgg acatcagtcc ccgctcccgc gccttgcgcg
     2161 cgagcaggcc ggcgccaatc atcaccgagg ggttggaggt gttggtgcaa ctcgtgatgg
     2221 cggcgatcag caccgcccca tccttcagct cgaacgagtg cccttcgagc gtcacgctgg
     2281 cgctgccttg cgcggccgta ttgcgcgcag cgcgctcttc ggccgacttc tttccgtgga
     2341 gctcgtacac cttgcgtgca gactgcaacg gcacgcggtc ctgcggccgc ttcggtccgg
     2401 cgaggctcgg ctccactttt cccagatcca gctccagcac atcggtgtag tcggctgccc
     2461 gatcaccgtt gttgcgccac aggccctggg ctttggcgta cgcactcacg agctcgatcc
     2521 gctcgcgggg acgaccggac agctcgagat agcggatcgt ttcttcatct atcgggaaga
     2581 tgccgcaggt gctgccgtat tcgggggcca tgttcgcgat cgtggcgcga tcggcgagcg
     2641 gcaggttcgc caggccatcg ccgaagaact ccacgaactt gtcgacaacc cccttcttgc
     2701 gcagcatctc ggtcacggtc aggacgaggt cggtggctgt cgcgccgggc ttgagacggc
     2761 cgttgagacg gaagccgatc acttgcggaa tcaacatcgt gaccggctgg ccgagcatgg
     2821 ccgcctcggc ttcgatgccg ccgacgcccc agcccagcac gcccaggccg ttgatcatgg
     2881 tggtgtgcga gtcggtgccg acgagcgtgt cgggatacgc gcgttgggtg ccgttgacct
     2941 gggcgtcgaa gatcacgcgc ccga
//
LOCUS       DASRHN010000033         8308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_176528, whole genome shotgun sequence.
ACCESSION   DASRHN010000033 DASRHN010000000
VERSION     DASRHN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176528"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFB37_01210"
     CDS             complement(<1..962)
                     /locus_tag="VFB37_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZO21089.1"
                     /translation="MNDVAKAAALPLGGSALHAYRRVLSYIRPHRGAFILGIFGGVIY
                     AAANTSFTGLAKFLFDGTLTRVLGNGPLTSMVGSAGSSIRDPRVILWLPLAVVIVFLI
                     RGLGDFTQTYFMGYVGRQIVCQLRREVYESVLRLPISYFDRNSSGALLSRLTYNSEQV
                     GQAATDSIVVIVRALLMILGSIVWLLFLNTRLALWSLIMGPFVGWLVSKINRHFRRYS
                     RRIQDSMGDVTRVAKESFEAPRLIKVYNAEGYQDRQFDLANTHNLRSNMKWILTKGLS
                     NPIVQLLSAIGAAVVLSLALEDVLHGRMSMGALVGFFTALASIT"
     gene            complement(959..1390)
                     /locus_tag="VFB37_01215"
     CDS             complement(959..1390)
                     /locus_tag="VFB37_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZO21090.1"
                     /translation="MNLKPRRHEEPEINVVSLIDVVLLLVVFFMLSSRFTEEGRLRVH
                     LPTASAVPTQRATTDPLVVTVTQQGSYRVNERELINSSEDTLRAALLKEAGSDRSRRI
                     TIRADARATHQSVITAMDVLGRLGFGEVNIATIKDESAGKP"
     gene            complement(1387..2079)
                     /locus_tag="VFB37_01220"
     CDS             complement(1387..2079)
                     /locus_tag="VFB37_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZO21091.1"
                     /translation="MWEIVRAGGPLMWPIILCSITAAAIILERLWTLQDRRVLPQELP
                     QKVWPLIEANQVNDKVIAALEQNSPLGKLLAAGLANRHRPREILMERLEDAGRHLVYD
                     LERYLNTLGTIAGIAPLLGLLGTVTGIIRSFNAIEQGGMGDPRALSGGIAEALIATVA
                     GLCVAIPALIAYRYFRGRVERIVVEMEKQAIRMADAVEASNVRQQQGPDSEQDARTLE
                     GATPLHRTRSRS"
     gene            2245..2781
                     /locus_tag="VFB37_01225"
     CDS             2245..2781
                     /locus_tag="VFB37_01225"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZO21092.1"
                     /translation="MPRRLIKRLTPSPGSLQNRWFLRPFAHRLADPQLWGLCRRGVTG
                     AFGAGLAICFIPLPVHLIVSSLLAVIFRLNIPVIIATVMLINPLTIVPVYYVAYRIGA
                     ALLGMPPEHFAFQMSWAWTHANLEPVWKAFLVGCLVCGVVAGVLGWMILELVWRWRVS
                     VRYRSRHEDSIEPHQAPR"
     gene            complement(2806..3516)
                     /gene="lolD"
                     /locus_tag="VFB37_01230"
     CDS             complement(2806..3516)
                     /gene="lolD"
                     /locus_tag="VFB37_01230"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HZO21093.1"
                     /translation="MTIPVLEAHDVQKSFKQGPVTLEVLQGVKLSVGTGERVAIIGAS
                     GSGKTTLLQILGGLDRPSSGQVRVDGQDIHALGEDARGMLRNRALGFVYQFHHLLPEF
                     SALENVAMPLLVRRTRIAEARSQARELLGRVGLEGRLDHRPYQLSGGERQRAAVARAL
                     VTRPKILLADEPTGNLDGRNAESVFALMLELNRERGTSLVVVTHDPRIASRMDRVFEI
                     EGGVLQERRSELEAPRAS"
     gene            complement(3523..4770)
                     /locus_tag="VFB37_01235"
     CDS             complement(3523..4770)
                     /locus_tag="VFB37_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZO21094.1"
                     /translation="MSTPYEWLIGTRYLRSTHRRGFVSFVALMSVFGLMLGVATLIVV
                     LSVMNGFERELRTRILSVTSHATLSGLDGPMSDWHSIQRAALKQPGVRAAVPYIEAQS
                     MIAAGANVTGATVRGVLPEEERKATGFAQHMISGRLEDLVPGAYRIILGDALARDLDA
                     HVGSQVILIAPEGTATPTGVVPRMRRFTVSGIFHSGMYEFDRALALVQMADAARLYRL
                     DDRVTGIRLALQDPLQAPTAVRRLAISLGGGFYVSDWTRSHVNFFRSIELTKSMMFVI
                     LLMIVAVAAFNIVATLVMIVKEKQADIAILRTLGAGPPNVLATFALQGIMIGLAGTLL
                     GAALGIVLSHNLEALVAGLEQLLHTQFLDASVYYMSDLPAYVEGGDVARVCGVAFLLC
                     AVATIYPAWRASRTAPAEALRHE"
     gene            complement(4773..6029)
                     /locus_tag="VFB37_01240"
     CDS             complement(4773..6029)
                     /locus_tag="VFB37_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZO21095.1"
                     /translation="MFRPLPLFVGLRYVRARTRKFFVSFITWTSLAGVCVGVAALIVI
                     LSVMNGLEGDLRDQLVSLSAHARVVARAGASAPSAAQWAAAERTVRAAEGVVAVSHYV
                     EIQGLAVRAPETMPILLRGIDPAAEAAVTDIGRSLVQGHLGDLTRGSNRVILGQVIAD
                     HLGLAPGDTLTVLVPDVSAAGVPAPRLREMTVAGVFEVGRVELDGNLVFGDIEDVRAL
                     TPAGSRTEGLRIRFRDALAAPVLSARLRPQLPPGFELFDWTQDNASYFRAVRIEKTMM
                     SLILMMIVGVAAFNIVSMLVMVVTDKRTDIAILRTLGTAPRGILGIFMTQGLLIGWVG
                     VALGVAVGLGIALHVDTLVPFLEHTFHFQIFNSDVYDMPRIPSDVRWINITMISAGAL
                     LLTALATVYPAIRAAHIAPAEALRYE"
     gene            6154..6435
                     /locus_tag="VFB37_01245"
     CDS             6154..6435
                     /locus_tag="VFB37_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21096.1"
                     /translation="MKTRMLVALTCACMAAGSALAETLVIDDHVTLRESTIDRPKRGT
                     TMTQVEAHFGAPTERHPTVGKPPITRWDYAGFSVFFEGDRVIHSVVTSG"
     gene            6728..7543
                     /locus_tag="VFB37_01250"
     CDS             6728..7543
                     /locus_tag="VFB37_01250"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZO21097.1"
                     /translation="MAADDFLRTRIEALMRAGAVRRKAVAAQLEAEIQRREEVMRQTF
                     RRYVSPRLADKILQDAQLRDSLLSTPDVRTHAVVLFADLRGFTGMSEQLAPHEVMPLL
                     NEYFSLLTEITFRHDGTVFHMAGDCLMLGFGVPLEQPDSSSRAILTAREMLSGFAELA
                     QSWKRRYQIEAGLGIGINEGDVVAGNVGSSAYMSYTIVGDTVNVASRLCQRARAGEIM
                     FSGAIKHSLDAHGLEIDALALPPMIVRGRTNPIDIYCVPLHDRLQVGPVPMEA"
     gene            7558..>8308
                     /locus_tag="VFB37_01255"
     CDS             7558..>8308
                     /locus_tag="VFB37_01255"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZO21098.1"
                     /translation="MLLKPPVPTSRLPRLRWHQLHGSAAALALAEATRADQRLYVVVA
                     DDARELERLSAELRFFAGEQLSLLTLPDWEVLPYDLFSPHPDITSQRLRTLFELPRLQ
                     HGCLVVTADVLMQRLPPRNYVQGRAFELEVGEQLPLEPFRQRLIEAGYVSVSQVASPG
                     EYAVRGSLFDVYPMGAATPLRIDLFDAEIEAIRRFDPETQRSLDSLQRIRLLPAREVP
                     LDPDGVRAFRRRFRTRFEGDPTRASIYRGVSE"
BASE COUNT         1456 a         2801 c         2658 g         1393 t
ORIGIN      
        1 tgcgtgatgc tggcgagggc ggtgaagaag ccgaccagcg cgcccatcga catgcggcca
       61 tgaagcacgt cctcgagcgc gagcgacagc acgaccgccg cgccgattgc gctgagcagc
      121 tgcacgatgg gattcgacaa acccttcgtc aggatccact tcatgttgga gcgcaggttg
      181 tgggtgttgg ccagatcgaa ctggcggtcc tgataacctt cggcgttgta gaccttgatg
      241 agtcgcggcg cctcgaagct ctccttggcc acccgcgtca catcgcccat cgaatcctgg
      301 atgcgccggc tgtagcggcg gaagtgccgg ttgatcttcg agaccagcca gccgacgaac
      361 ggccccatga tcagcgacca gagcgcgagc cgcgtgttga ggaacagcag ccacacgatc
      421 gatcccagga tcatgagcag cgcccgcacg atgaccacga tcgagtcggt cgcggcctgc
      481 cccacctgtt cgctgttgta ggtcaggcgc gacagcagcg ccccggagga attgcggtcg
      541 aagtagctga tcggcagccg cagcacgctc tcgtagacct cgcgccgcag ctgacacacg
      601 atctgccggc cgacgtatcc catgaagtag gtctgcgtga agtcccccag gccacggatc
      661 agaaacacga tgacgacggc gagcggcagc cacagaatca cgcgcggatc gcggatgctg
      721 gagccggcac tgccgaccat gctcgtcagg ggtccattgc cgagcacccg ggtgagggtc
      781 ccgtcgaaga gaaacttggc cagtcccgtg aagctggtgt tggctgcggc gtagatcaca
      841 ccgccgaaga tgccgaggat gaacgctccg cggtggggcc ggatgtagct cagcacgcgc
      901 cgataggcat gcagagcgct cccgcccaga gggagcgctg ctgccttcgc tacatcgttc
      961 aaggcttacc tgccgactcg tccttgatgg tcgcgatgtt gacttccccg aaaccgaggc
     1021 gcccgagaac gtccattgcg gtgatgaccg actgatgcgt ggcgcgcgca tcagcgcgaa
     1081 tggtgatccg gcggctgcgg tcgctgccgg cctccttgag cagcgccgcg cgcagggtgt
     1141 cctcactgga gttgatcagc tcgcgctcgt tgacgcggta actcccctgc tgagtgacgg
     1201 tcaccaccag cggatcggtc gtggcgcgct gcgttggcac ggcactcgcg gtaggcagat
     1261 gcacccgtag ccgcccctct tcggtgaagc ggctggagag catgaagaac accaccagca
     1321 gcaggaccac gtcgatcagc gagacgacgt tgatttccgg ctcctcgtga cggcgcggct
     1381 tcaaattcat gagcggctgc gcgtgcgatg caacggagtg gcgccctcga gggtgcgtgc
     1441 atcctgctcg ctatcgggac cctgttgctg gcgcacgttg gaggcttcca ccgcatcggc
     1501 catgcggatg gcctgctttt ccatctccac cacgatgcgc tcgacgcggc cgcggaagta
     1561 gcgataggca atcagggcgg ggattgcgac gcacaaacct gccacggtcg cgatcaaggc
     1621 ttccgcaata ccgcccgaga gggcgcgcgg gtcgcccatg cccccctgct cgatggcgtt
     1681 gaaggagcgg atgatgcccg tgacggtgcc gagcaggccc agcagcgggg cgatgccggc
     1741 aatcgtcccc aaggtgttca ggtagcgctc gaggtcatag acgaggtgcc gtccggcatc
     1801 ctccaggcgc tccatgagga tctcccgcgg gcggtgccga ttggccagcc ccgcagccag
     1861 cagcttcccc aggggcgagt tctgctccag ggccgcgatc accttgtcat tgacctgatt
     1921 ggcctcgatc aggggccaga ccttctgcgg gagctcctgg ggcaatacgc ggcgatcctg
     1981 aagcgtccac aaccgttcaa ggatgatcgc cgcggcggtt atggagcaca gaatgatcgg
     2041 ccacatcagc gggccgccgg cccgcacaat ttcccacatc cgagtgaccc caagccgtga
     2101 cgacgacggg gcatcttaca ggacccccga aagcgctcaa cgtatgtgca gggcctcgga
     2161 caggctcccg gcgggcgccc gctgaagaca taacgtggct ggaatcattg tgatcaaatg
     2221 tttctgctag agtccaggcc aaaaatgccc cgcagactca tcaagcgcct cacgccctcg
     2281 cctggatccc tgcaaaaccg gtggttcctg cgcccgttcg cgcaccggct ggccgacccg
     2341 cagttgtggg gactgtgccg ccggggcgtc accggcgcct tcggcgcggg cctggccatt
     2401 tgcttcatcc ccctgcccgt tcacctgatc gtctcgagcc tgctggccgt gatctttcgg
     2461 ctgaacatcc ccgtgatcat tgccacggtc atgctgatca atccgctcac catcgtgccg
     2521 gtctattacg tggcctatcg gatcggagcg gcgctgctcg gcatgccgcc ggagcatttc
     2581 gccttccaga tgagctgggc ctggacgcat gcgaacctcg aacccgtatg gaaggcattc
     2641 ctggtgggct gcctggtctg cggggtcgtc gccggcgtcc tcggctggat gatcctcgag
     2701 ctcgtgtggc gatggcgggt ctcggtgcgc tatcgcagtc gccacgagga cagcatcgag
     2761 ccgcaccagg cgccacgttg agcgggccta cacggccacg tccgctcaag aggcgcgggg
     2821 tgcctccagc tcgctcctgc gctcctgcag cacgccaccc tcgatctcga agacccgatc
     2881 catgcggctc gcgatacgcg gatcgtgggt gaccacgacc aggctcgtgc cgcgctccct
     2941 gttgagctcc agcatgagcg caaacacgct ctccgcgttg cggccatcca ggttgccggt
     3001 gggctcatcg gcgaggagga ttttgggacg cgtcacgagc gcccgcgcta ccgcggcccg
     3061 ctgccgctcc ccacccgaga gctggtacgg gcgatggtcc agccggcctt cgaggccgac
     3121 ccggccaaga agctcgcgcg cctggctgcg cgcttccgca atccgcgtgc gccgcacgag
     3181 caggggcatg gccacattct cgagcgcgga gaactccggc agcagatgat ggaactgata
     3241 cacgaatccg agcgcccggt tgcgcagcat cccgcgcgca tcctccccca gggcgtgaat
     3301 gtcctgtccg tccacccgga cttgccccga gctcgggcga tccagacctc cgagaatctg
     3361 caacagggtg gtcttgcccg agccggaggc gccgatgatc gcgacccgct cgcccgtgcc
     3421 cactgagagc ttcaccccct gcaggacctc gagtgtgacg ggcccctgct tgaagctttt
     3481 ctggacatca tgggcttcca acaccggtat cgtcatcgaa gctcactcat gccgcagggc
     3541 ctctgccggg gcggtacgcg aggcccgcca ggcgggatag atggtcgcga ccgcacacag
     3601 cagaaacgcg accccacaaa ctcgcgcgac atccccgccc tccacgtaag cgggtaagtc
     3661 gctcatgtag taaacgctcg catccaggaa ctgggtgtgc agcagctgct ctagtccggc
     3721 gaccagcgcc tccaggttat gcgacagcac gatcccgagc gcagcaccga gcaaggttcc
     3781 ggcgagcccg atcatgatgc cctgaagcgc gaaggttgcc agcacgttgg gcggccctgc
     3841 cccgagcgtg cgcaggatgg cgatgtcggc ctgcttctcc ttcacgatca tgacgagcgt
     3901 ggcgacgatg ttgaaggccg ccaccgccac gatcatcagc aggatcacga acatcatgga
     3961 cttcgtgagc tcgatggagc ggaagaaatt gacgtggctg cgcgtccagt cgctgacata
     4021 aaaaccgccg cccagactga tggcgaggcg tcgcaccgcc gtgggcgcct gcagtgggtc
     4081 ctgcagcgcc aggcggatcc ccgtgacccg atcatccagc cggtaaagac gcgccgcatc
     4141 ggccatctgc accagggcga gcgcgcgatc gaactcgtac attccggagt ggaagatgcc
     4201 gctcaccgtg aagcggcgca tgcgcgggac caccccggtg ggcgtcgcgg tgccctcggg
     4261 ggcgatcaaa atcacctggc tgccgacatg tgcatcgagg tcccgggcga gcgcatcgcc
     4321 caggatgatg cggtaggcgc ccggcaccag gtcctcgagg cggccggaga tcatgtgctg
     4381 tgcgaatcca gtggccttgc gctcctcctc cggcagcacg cctcttacgg tcgcgccagt
     4441 cacgttggct ccggcggcga tcatcgactg cgcctcgatg tagggaacgg ccgcccgcac
     4501 gcccggctgc ttgagggctg cccgctggat cgagtgccag tcgctcatcg gtccgtcgag
     4561 gccgctgagc gtggcatggg aggtcacgct cagaatgcgg gtgcgcagct cgcgctcgaa
     4621 gccgttcatg accgagagca ccacgatcag cgtggcgaca cccagcatca agccgaacac
     4681 cgacatcagg gccacgaacg acacgaagcc gcgacgatgc gtcgagcgca ggtagcgcgt
     4741 gccgatgagc cattcgtaag gagtgctcat ggctactcgt agcgaagcgc ttcggcgggg
     4801 gcgatgtgcg cggcgcgaat ggccggatag acggttgcga gcgccgtgag cagcagtgca
     4861 ccggcgctga tcatggtgat gttgatccag cgcacatccg atgggatgcg gggcatgtcg
     4921 tacacgtcgg agttgaagat ctggaaatgg aaggtgtgct ccagaaacgg cacgagcgta
     4981 tcgacgtgca aggcgatgcc caggcccacc gcgacgccca gcgcaacgcc gacccaaccg
     5041 atgagcagac cctgggtcat gaagatgccg aggatgccgc gtggcgcggt tcccagggtc
     5101 cgcaggatgg cgatgtcggt gcgcttgtcg gtgacgacca tgacgagcat cgacacgatg
     5161 ttgaaggcgg ccacgcccac gatcatcatc aggatgagcg acatcatggt cttctcgatt
     5221 cgcacggcgc ggaaatagct ggcgttgtcc tgcgtccagt cgaacagctc gaagcctggc
     5281 ggcaactgcg gccgcagcct ggcagagagc accggcgccg ccagcgcatc ccggaaccgg
     5341 atccgcagac cttccgtgcg ggagcctgcc ggagtcaggg cgcgcacgtc ctcgatgtca
     5401 ccgaacacga ggttgccgtc gagctcaaca cgaccgacct cgaagactcc cgcgacagtc
     5461 atttcccgca accgcggagc cggcactcct gccgccgata catccggcac gagcaccgtc
     5521 aaggtgtcac ccggggcaag ccccaggtga tccgcgatca cctggcccag aatcacgcgg
     5581 ttggagccgc gggtgagatc gccaagatgg ccctggacca gcgagcgccc gatgtcggtc
     5641 accgctgctt ccgccgcagg atcgattcca cgcagcagga tcggcatcgt ctcgggtgcc
     5701 cgcaccgcga gcccttgtat ctcgacgtag tgcgataccg ccaccacccc ttcggcggcg
     5761 cgcacggtgc gctccgcggc cgcccactgc gcagccgacg gagctgatgc gcccgcgcga
     5821 gctacgaccc gcgcgtgcgc actgagggag accagttggt cacgaagatc gccctcgagc
     5881 ccgttcatca ccgacaggat cacgatcagc gcggcgaccc ccacgcaaac cccggcaagg
     5941 gaggtccacg tgatgaacga gacaaagaac ttgcgggtcc tggcacgcac gtagcgcagg
     6001 cccacgaaca gcggcagcgg gcgaaacatc gtcgcgattt aaccacagtt atggccggga
     6061 cctgtgtcac attcccgacg atcgccggct gcgggcggct ttgacagaac gaccccggtg
     6121 ctatagtcgc ccttgcggca ggagttcaac gccatgaaaa cccgaatgct cgtcgcgctg
     6181 acatgcgcct gcatggcagc cggatcggcg ctcgccgaga cactcgtgat cgacgatcac
     6241 gtgaccttgc gcgaatccac gatcgaccgc ccgaagcggg gcacgaccat gacccaggtc
     6301 gaggcgcatt tcggcgcccc caccgagcgt catcccacgg tcggcaaacc gcccatcacc
     6361 cgctgggact acgctggttt ctcggtgttc ttcgaaggcg accgcgtgat tcattcggtg
     6421 gtcaccagcg gctgacacgc cgcgttcacc cgaccgcgct ccctcgtcag attcacacgg
     6481 ccgcccgggt gcggccgcgt tcatctctgc cgccacaccg atcctccagt cttgtcacgg
     6541 acccgctctg ccgccgcgtt gcggcatttg acagtgggca agcccggctg cggatgatag
     6601 ccttcgccgc gaaatcctcc cgcaagcccg tgcgtgcatg gaagttctgc ccgctcgggc
     6661 ccgaggagca gagccgctga tcgcacctcg cggtgaggtt cagccaagac agatagggga
     6721 tagcaaggtg gcggcagatg actttctgag gacccgtatc gaggctctca tgcgcgcggg
     6781 cgccgtccgg cgcaaggcgg tagccgcgca gctcgaagcg gagatccagc gccgcgagga
     6841 ggtcatgcgc cagacgttcc ggcgctatgt ttccccgcgg ctggcggaca agatcctgca
     6901 ggatgcgcag ctgcgcgaca gcctgctctc caccccggat gtgcgcacgc atgcggtcgt
     6961 actgttcgcc gatctgcgcg gcttcaccgg catgtccgag cagcttgcac cgcatgaggt
     7021 gatgccgctt ctgaacgagt atttctcgct gttgaccgag atcacctttc gccacgacgg
     7081 cacggtgttt catatggcgg gcgactgcct gatgcttggt ttcggtgtgc cgctcgagca
     7141 gcccgacagc tcctcgagag cgatcctgac ggcgcgcgag atgctcagtg gcttcgccga
     7201 gctcgcccag tcgtggaagc ggcgctacca gatcgaggcg ggactcggca tcggcatcaa
     7261 cgagggcgat gtggtcgcgg gcaacgtcgg gtcctccgcc tacatgagtt acaccatcgt
     7321 aggtgatacc gtgaacgttg cttcgcggtt gtgtcagcgc gcccgcgccg gtgagatcat
     7381 gttctcgggc gccatcaagc attccctcga tgcccacggt ctcgagatcg acgcactggc
     7441 cctgccgccg atgatcgttc gcgggcgcac caatccgatc gacatctact gcgtgccgct
     7501 gcacgatcgc ctgcaggtcg gccccgtgcc catggaagcc tgaggccggc tcgatacttg
     7561 ctcctcaagc ccccggttcc cacctcccgt ctccctcgcc tccgctggca tcagctccac
     7621 ggcagcgctg ccgcgctcgc gcttgccgag gctacccggg ccgatcagcg gctctatgtg
     7681 gtggtagcgg atgatgctcg agagctcgag cgtctctcgg ccgagctgcg cttcttcgcc
     7741 ggcgagcagc tttcgctgct gacactgccg gactgggagg tgctgcccta cgaccttttc
     7801 tctccgcacc ccgacatcac ctcccaacgg ctgcgcaccc tgttcgagct gcctcgtctt
     7861 cagcatggct gcctggtcgt gacggccgat gtgttgatgc agagattgcc gccgcgcaat
     7921 tacgtgcagg ggcgcgcgtt cgagctcgag gtgggtgagc agctgcccct cgagcccttc
     7981 cggcaacggc tcatcgaggc cggctacgtc agcgtgagtc aggtcgcaag tcccggcgag
     8041 tatgccgtgc gcggctcatt gttcgatgtc tatcccatgg gagccgccac gccgctgcgc
     8101 atcgatctgt ttgacgctga gatcgaagcc atccggcgct tcgaccccga gacgcagcgc
     8161 tcgctcgact cgctgcagcg catccgcctg ctgccggccc gcgaagtccc gctcgatccg
     8221 gacggcgtgc gcgcgttccg tcgccgcttc cgcacgcgct tcgaaggtga tcccacccga
     8281 gcgtccatct accgcggagt cagcgagg
//
LOCUS       DASRHN010000034         6433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_176557, whole genome shotgun sequence.
ACCESSION   DASRHN010000034 DASRHN010000000
VERSION     DASRHN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176557"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFB37_01260"
     CDS             <1..1065
                     /locus_tag="VFB37_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO21099.1"
                     /translation="PEETIQDDRKGKIVLRRKPVGVVGSITPWNWPMMIAVWHIMPAV
                     RVGCTIVIKPSPFTPLSTLRLVELINEVLPPGVVNAVTGEGDVGAHLASHPGIDKVVF
                     TGSVATGKKIMGGASQTLKRVTLELGGNDAGIVLPGTNIEPLLEKLFWGCFINAGQTC
                     AALKRLYVHEDQYEEVVQKFSEYVAKIPVGNGLDPKNLIGPLSNRMQFDKVVSLVEDA
                     RSSGARIVTGGVRPQAPGFFYPLTVIANAKDEMRVVKEEQFGPVIPLVKYKTVEEALR
                     RANSLDVGLGGSVWGDDPQEAAKYASQLECGTAWVNQHGTLHPLAPFGGVKCSGIGVE
                     FNVDGLKEYTTVQVVNVALQ"
     gene            1075..2571
                     /gene="hpaB"
                     /locus_tag="VFB37_01265"
     CDS             1075..2571
                     /gene="hpaB"
                     /locus_tag="VFB37_01265"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HZO21100.1"
                     /translation="MSNPSGGGARRGAQVLQRLREQPPALWYAGEPVKDVTVHPAFKG
                     GVHTLARLYDLQWEKADITLYDSPTSGQKVARSFMMPRTREELISVSRAMQVWEDYTH
                     GMMGRVPDYINRAMTGYAAGAPFLAEADPRFGENAVRYYEYLRENDLCLTHTLIPPQA
                     NRAVSSAKQADPFLAARIKEETDAGMVIRGCRMLATLPISDELMVFPSTLLKSPEEDA
                     PYAFGFSIPSSTPGLRFLCRESVDYGRSHFDHPLGSRFEEMDAVVIFDDVFVPWENVF
                     LHRDIQRCNQAYARTGAVAHMTHQVVVKNIAKCEFLLGLASLMVNAIGAEIFQHVQEK
                     VAEIWVNLETMKAFLRAAEADAALDEWGVMRPDWNPLDAARNLFPRLYPRMIEIIQQI
                     GASGLVAMPTAQDVRGPLAEDIRKYYQAARAEAFERIPLFRLAWDASISAFAGRQVLY
                     ERFFFGDPVRMAGALVAGHNSQIQQYAEKVRAFVLAGRDEAFAAASRS"
     gene            2568..3122
                     /locus_tag="VFB37_01270"
     CDS             2568..3122
                     /locus_tag="VFB37_01270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZO21101.1"
                     /translation="MSRPPPPLDTRRFRDTLGLFATGVAVIVARAGEEVHAMTVNAVS
                     SVSLDPMLVLFCPGKTTRLARDVGRIAGFTINFLRREQQALSTYFAGGWKEPSAPPYR
                     FVTSRSALRLEGSLASLDCEMERVTEAGDHWLVIGRVVHVHLGIQPHHPLVFFKGQYR
                     GVDFTESAPAPDLVNIHEVPFHFY"
     gene            3132..4622
                     /gene="glpK"
                     /locus_tag="VFB37_01275"
     CDS             3132..4622
                     /gene="glpK"
                     /locus_tag="VFB37_01275"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZO21102.1"
                     /translation="MSEPVLLAIDQGTSSSRAIGFSTRGEVLALEQQPFEQLYPASGW
                     VEHDPEVIWATALSTTREALRRLRDKGLSVAAIGVTNQRETTLLWSRRTGVPFHNAIV
                     WQDRRTAEHCRRLQEAGQEEQVALKTGLRLDPYFSATKLAWMLENVPAAREAAREGDL
                     AFGTVDSFLIWRLTGGRLHVTDATNASRTALFDIHAGRWDPQLCDLFGVPLSCLPEVR
                     DSAGDLGSTDSGLFGESLPIRGVAGDQQAALIGQAGFAPGDVKSTYGTGAFVVLNTGN
                     RPLRSRNRLLSTIAYQLGGKRTYALEGSILSAGSTIQWLRDGLGLITRAAESEALAQS
                     IPDSAGVYLLPAFTGLGAPYWDPEARGAIVGLTRASSRAEIVRAGLDSVVYQTRDLLD
                     AMAADGERPASLKVDGGMAGNAFFLQRLADILGMPILRPRMTESTAFGAACLAALGCG
                     IYRSVEEIAALWQADVRCEPRLDAAAREREMSGWRQALQRVRKSGL"
     gene            4657..4947
                     /locus_tag="VFB37_01280"
     CDS             4657..4947
                     /locus_tag="VFB37_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HZO21103.1"
                     /translation="MAKIPHLLVHDRRDNVGVVVVENLAAGTDMFCVVTEDNSEFRAV
                     CAQDVPIGHKIALRDLAVGDTVIKYGEDIGRIVAPVRRGEHVHTHNLKTKRW"
     gene            4953..6128
                     /locus_tag="VFB37_01285"
     CDS             4953..6128
                     /locus_tag="VFB37_01285"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HZO21104.1"
                     /translation="MTSSNRTIWGYRRENGRVGVRNHVAILPLDDISNAASEAVAGHI
                     RGTIALPHAYGRLQFGEDLKLHFRTMIGTGANPNVGACVVIGIEPGWTQKVVEGIAQT
                     GKPVAGFSIEGNGDIATVAAASRKAKEFVHRVSEQVRVECPLSEVWIAAKCGESDTTT
                     GLASCPTVGNMYDKLVPQGLYGCFGETSELTGAEHLAAARAISPEIGAKFMQTWSAYQ
                     REVIEAHKTSDLSDSQPTKGNIAGGLTTIEEKALGNLEKIGRKVRFIDVLAPAEAPAR
                     GAGLYFMDTSSAAAECCTLQAAAGYVLHIFPTGQGNVIGNPIMPVLKISGNPRTVRLM
                     SEHIDLDVSGIIRREITIEQAGDALIDAMVRTANGRLTSAEALGHVEFVMTKLYRSA"
BASE COUNT         1114 a         2041 c         2148 g         1130 t
ORIGIN      
        1 ccggaggaga cgattcagga tgaccgcaag ggcaagatcg tcctgcggcg caagccggtc
       61 ggcgtggtgg gatcgatcac accctggaac tggccgatga tgatcgccgt gtggcacatc
      121 atgccggccg tgcgggtggg ctgcacgatc gtgatcaagc cctccccgtt cacgcctctt
      181 tccactctcc ggctggtcga gctcatcaac gaagtcctgc cgccgggggt cgtcaatgcc
      241 gtgacgggcg aaggcgatgt cggcgcccac ctcgccagtc atccggggat cgacaaggtg
      301 gtgttcaccg gctcggtcgc caccggcaag aagatcatgg gcggagcctc gcaaacgctc
      361 aagcgggtga cactcgagct gggcggcaac gatgccggca tcgtcctgcc cggcacaaat
      421 atcgagccgc tgctcgagaa gctgttctgg ggctgcttca tcaatgccgg acagacctgt
      481 gcggcgctga agcggctgta cgttcacgaa gaccagtacg aggaggtggt gcagaaattc
      541 tccgagtacg tcgccaagat cccggtcggc aacgggctcg atccaaagaa tctgatcggc
      601 ccgctctcga atcggatgca gttcgacaag gtcgtctcac tggtcgagga cgcgcgctcc
      661 agcggcgcac ggatcgtgac cggtggagta cgcccgcagg ccccgggctt cttctatccg
      721 ctcacggtga tcgccaacgc caaggatgag atgcgtgtgg tcaaggaaga gcagtttggc
      781 cccgtcattc cgctggtgaa gtacaagacg gtggaagagg cactgcggcg cgccaactcg
      841 ctcgatgtcg gtctgggcgg ctcggtatgg ggcgatgatc cgcaggaggc ggcgaagtat
      901 gccagccagc tcgaatgcgg caccgcctgg gtcaaccaac acgggacgct gcacccactg
      961 gcgccgttcg gcggcgtgaa atgctcggga atcggtgtcg agttcaacgt ggacgggctg
     1021 aaggaataca ccaccgtgca ggtggtcaat gtcgcgcttc agtgagggcc ttccatgagc
     1081 aacccatcag gcgggggcgc acgccgcggg gcccaggtgt tgcagaggct gcgcgagcag
     1141 cctccagcgc tgtggtacgc cggggagccg gtcaaggacg tcaccgtgca tccggcattc
     1201 aagggcggcg ttcataccct ggccaggctc tatgacctgc aatgggagaa ggccgacatc
     1261 acgctgtacg actcaccgac gagcggccag aaggtcgccc gctcgttcat gatgccgcgc
     1321 acgcgtgagg agctgatcag cgtgagtcgc gcgatgcagg tgtgggagga ctacacgcac
     1381 gggatgatgg ggcgcgtacc ggactacatc aaccgcgcga tgaccggtta tgccgccggc
     1441 gcgcccttcc tcgcagaagc cgacccgcgc ttcggcgaaa acgccgttcg ttattatgag
     1501 tacctgcgcg agaacgatct ctgtttgacg cacacgctca tccctccgca ggcgaatcgg
     1561 gcggtgagct cggcgaagca ggccgacccg ttcctcgccg cacggatcaa ggaggaaacc
     1621 gatgccggca tggtgattcg cggctgccgc atgctggcga cgctgcccat ctccgatgag
     1681 ctcatggtgt ttccctcgac gctgctgaag agtcccgagg aggatgcacc gtatgcattc
     1741 ggcttctcga tcccgagctc gacgccggga ctgcgctttc tgtgccgcga aagcgtggac
     1801 tacggccgct cgcatttcga tcacccactc ggctcgcggt tcgaggaaat ggatgccgtc
     1861 gtcatcttcg atgacgtgtt cgttccgtgg gaaaacgtat ttctgcatcg ggacatccag
     1921 cgctgcaacc aggcctatgc ccgcaccggc gcggtcgctc acatgacaca ccaggtcgtg
     1981 gtgaagaaca tcgccaagtg cgagttcctc ctcggcctcg cctcgctgat ggtgaatgcc
     2041 atcggcgcgg aaatcttcca gcacgtgcag gagaaggtgg ccgagatctg ggtcaatctc
     2101 gagaccatga aggccttttt gcgtgccgcc gaggccgatg cggcgctcga tgaatggggc
     2161 gtgatgcgcc cggactggaa cccgctcgat gccgcacgca atctgtttcc gcgcctgtac
     2221 ccgcgcatga tcgagatcat ccagcagatc ggcgcgagtg gcctcgttgc catgcccacc
     2281 gcgcaggatg tccgcggacc gctggcggag gatattcgca agtattatca ggctgcacgc
     2341 gccgaggcct tcgagcgcat cccgctcttc cggctcgcct gggatgcctc gatctccgcc
     2401 ttcgccggtc gccaggtgtt gtacgagcgt ttcttcttcg gggatccggt gcgcatggcg
     2461 ggggctctcg tggccggcca taattcgcag atccagcagt atgccgagaa ggtacgtgcg
     2521 ttcgtgctag ccggaagaga tgaggcattc gctgcggcct cgcgctcgtg agcaggccgc
     2581 ccccgccgct ggatacccgc cggtttcgcg ataccctcgg tctgttcgcg acgggcgtgg
     2641 cggtgatcgt cgcgcgagcg ggcgaggagg tgcacgccat gacggtgaat gcggtgtcct
     2701 cggtatcgct cgatccgatg ctggtgctgt tctgcccggg caagacgacg cggctcgcgc
     2761 gcgatgtcgg gcgtatcgcc ggattcacga tcaacttcct gcgacgtgag cagcaggcgc
     2821 tgtccaccta tttcgccgga ggatggaagg agccgagcgc accgccctac cgcttcgtca
     2881 cctcgcgcag cgcgctgcgc ctggagggca gtcttgcatc gctcgattgc gagatggagc
     2941 gcgtcacgga agccggcgat cactggctcg tgatcggccg tgtcgtgcac gtgcatctgg
     3001 gcattcagcc gcaccacccg ctcgtgttct tcaaggggca gtaccgcggc gtggatttca
     3061 ccgaaagtgc cccggcaccg gacctggtga acattcacga ggttcctttc catttctatt
     3121 gaggcgccgc catgagcgag cccgttctgc tggcgatcga tcagggcacg agctcgagcc
     3181 gggccatcgg cttctcgacg cgcggcgagg tgctggcgct cgagcagcag cccttcgagc
     3241 agctctatcc cgcctccgga tgggtcgagc acgaccccga agtgatctgg gccaccgcgt
     3301 tgtcgaccac gcgcgaagcg ctgcgccgcc tgcgtgacaa gggtctgtca gttgccgcca
     3361 tcggcgtcac gaaccagcgc gagacgacgc tcctgtggag tcgccggact ggggttccgt
     3421 ttcacaacgc catcgtctgg caggaccgcc ggaccgccga gcattgccgg cgtctgcagg
     3481 aggcgggcca ggaggagcag gtggccctga agaccgggct gcgtctggat ccctattttt
     3541 ccgccacgaa gctcgcgtgg atgctcgaga acgtgcccgc tgcgcgcgag gcggcccgcg
     3601 agggcgacct cgccttcggc accgttgaca gctttctcat ctggcgcctc accgggggcc
     3661 ggttgcatgt gaccgatgcc accaatgcca gccgcacggc cttgttcgac atccacgccg
     3721 gccgctggga cccgcagctg tgcgatctat tcggcgtgcc gctgagctgc ctccccgagg
     3781 tgcgggacag cgccggcgat ctcggcagca cggacagcgg tcttttcggg gaatcgcttc
     3841 ccatccgcgg cgtcgcggga gatcagcagg ctgcgctcat cggccaggcg gggttcgcgc
     3901 caggggatgt gaaaagcacg tacggcaccg gggcatttgt cgtactcaac accggcaatc
     3961 ggccactgcg ctcgcgcaac cggctgctga gcacgatcgc ttatcagctc gggggcaagc
     4021 gcacctatgc gctcgagggg tcgatcctct cggcgggatc gacgatccag tggcttcgcg
     4081 acggcctcgg cctgatcacg cgagccgccg agagcgaagc gctcgcgcaa tcgatccccg
     4141 acagcgcagg cgtgtatctg cttccggcgt tcacggggct cggcgctccc tactgggatc
     4201 ccgaggcgcg cggcgccatc gtcggcttga cccgtgcctc gagtcgcgcc gagatcgtcc
     4261 gggctgggct cgacagcgtg gtctaccaga cgcgggatct gctcgatgcc atggccgcgg
     4321 acggcgagcg tccggcttcg ctgaaggtcg atggcggcat ggccgggaat gctttcttcc
     4381 tacagcgcct ggcggacatt ctgggcatgc cgattctgcg gccgcggatg acggaatcca
     4441 cggctttcgg cgcggcctgc ctggccgctc tcggctgcgg gatctaccgc tcggtagagg
     4501 agattgcagc gctgtggcag gcggatgtgc gctgcgagcc gcgactggat gcggccgctc
     4561 gcgagcgtga gatgagcggc tggcggcagg cgctgcagcg ggtgaggaag agcggcctat
     4621 aatggccgct ccgcagaggg acccagacgg agcgtcatgg ccaagatacc ccacctcctc
     4681 gtgcacgatc ggcgcgacaa cgttggtgtc gtcgttgtcg agaacctcgc ggcgggcact
     4741 gacatgttct gtgtggtcac cgaagacaac agtgagtttc gcgcagtctg cgcgcaggac
     4801 gtgcccatcg gccacaagat cgctctgcgg gacctggcgg ttggggacac cgtgatcaag
     4861 tacggcgaag atatcggacg catcgtggct ccagtgcggc gtggcgagca cgtgcacact
     4921 cacaatctga agaccaagcg ctggtaggga tgatgacaag ctcgaacagg accatctggg
     4981 gctaccggcg cgagaatggt cgcgtcggtg tgcgcaatca cgtcgcgata ttgccgctcg
     5041 atgacatctc caatgctgcg agcgaagccg tcgcaggcca tatccgtgga acgatcgcct
     5101 tgccgcatgc ctacgggcgc ctgcagttcg gtgaggacct gaagctgcac ttccggacga
     5161 tgatcggcac gggggccaac cctaatgtcg gggcttgcgt cgtgatcggg atcgagccgg
     5221 ggtggactca gaaggtcgtg gagggtatcg cccagaccgg caagccggtc gccggtttct
     5281 cgatcgaagg caatggggat atcgccacgg tggcggccgc ctcgcgcaag gcgaaggaat
     5341 tcgtgcatcg ggtttccgag caggtccgcg tggagtgccc gctgagcgag gtgtggatcg
     5401 ctgccaagtg cggcgaaagc gacaccacga cgggcctcgc ttcctgtccc acggtcggca
     5461 acatgtacga caagctcgtg ccgcagggcc tgtatggatg cttcggtgaa acctccgagc
     5521 tcaccggcgc cgaacacctg gctgccgcac gcgcgatctc gccggagatc ggggccaagt
     5581 tcatgcagac ctggtccgcc taccagcgcg aggtcatcga ggcacacaag acatccgacc
     5641 tttccgatag ccagcccacc aaaggcaaca tcgccggcgg gctgacgacg atcgaggaga
     5701 aggcgctcgg caacctggag aagatcggcc gcaaggtgcg attcatcgac gttctggccc
     5761 cggccgaggc cccggctcgc ggcgccggac tgtacttcat ggacacctcc tcggcggccg
     5821 ccgaatgctg cacgctgcag gccgcggccg gttacgttct gcacatcttt ccgaccggcc
     5881 agggtaatgt gatcggcaat ccgatcatgc ccgtcctcaa gatcagcggg aatccgcgga
     5941 ccgttcgtct gatgagcgaa cacatcgacc tggatgtctc gggcatcatc cgtcgcgaga
     6001 tcacgatcga gcaggcgggt gatgcgctca tcgatgccat ggtgcgcacc gccaacggcc
     6061 ggctcacctc cgccgaggcc ctggggcacg tcgagttcgt catgacgaag ctctacagga
     6121 gcgcctgagc tccatcggat ccgggccgcg agtcacatca gcgcgcccgg gttggcgcgg
     6181 cggacagtag cccgcgcgtg catccggacg gacatctggg ccgtataatg cgcgtctgcc
     6241 aacagaagcg gatccttgca tgctgcgctt gctccaggcc acgacaatca ccctcctcct
     6301 ggcacttctt gctgggatgc ccgccagcgc gcgtcccatc gactggcagt ccaaatggag
     6361 cgaggcggcc tttgctcagg ccgcacgcga acacaagttc gtgctgctgg atctgcacgc
     6421 cgtgtggtgc cac
//
LOCUS       DASRHN010000035         9944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1493422, whole genome shotgun sequence.
ACCESSION   DASRHN010000035 DASRHN010000000
VERSION     DASRHN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493422"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFB37_01290"
     CDS             complement(<1..434)
                     /locus_tag="VFB37_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZO21105.1"
                     /translation="MTRLWDKGTELSDSVLRYTAGEDHTLDERLVRYDVQASIAHVEM
                     LYAQKLISAEDLHAIRDGLAHLALEHTRGLWQIELQDEDGQTALERRLTARIGAAGAR
                     VHLGRSRNDQVLAAVRLYLRDATKELSGRAVQVAEALEGLAHR"
     gene            complement(431..1384)
                     /gene="argB"
                     /locus_tag="VFB37_01295"
     CDS             complement(431..1384)
                     /gene="argB"
                     /locus_tag="VFB37_01295"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZO21106.1"
                     /translation="MIMHRGEQALAIRALKSAAPYIRMYKGKTFIVKSGGGVFADAAT
                     TRVLIEQIAILHYFGVRVVVVHGGGPQLTDLSEALGVPTRMVEGRRVTDQKSIDVTAM
                     VLNGLINTRILGICRDLNIDAVGISGVDAGLVRAHKRAPVRIGSTGETVDYGFVGDID
                     AVDPTVIRKLLDNGLMPVVSPLSADQSGTLLNINADTVAAAIGAALGAEKLILCTGAP
                     GILERVGDAGSLISYTDLRGLKRLKDEGRLLEGMLPKAKAIEEAIRGGVRRVHVISYK
                     SPEGILGEVFTNEGTGTLIVADINALTLAEQHLAQGSTDIP"
     gene            complement(1391..2392)
                     /locus_tag="VFB37_01300"
     CDS             complement(1391..2392)
                     /locus_tag="VFB37_01300"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HZO21107.1"
                     /translation="MKRFLDLGDFSREELGSLLELARRLEIRPEPQALSGKVLGLLFF
                     NPSLRTLASFQSGMARLGGSSFVITPGQGTWQLETRTGVVMNGAAAEHVREGIPVLAS
                     YCDALGIRAFAEGKDLAADLAESNFKSMAELVDKPLINLESAANHPCQALADWKTLDD
                     LGIPRQSRFVLSWVYHPRALPLAVPAAALHMGAMRGMEVIVLRPAGFELPPVIMDKAR
                     RAAELSGGSVRETSDRGEALTGASVVYAKEWGATSCYGDTLADARLRAELTDWCVRDE
                     WFAPAAADCRLMHCLPVRRNTAVADEVLDGPRSIVQRQAFNRLIVQMAVLYQMLKRG"
     gene            complement(2389..3564)
                     /locus_tag="VFB37_01305"
     CDS             complement(2389..3564)
                     /locus_tag="VFB37_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO21108.1"
                     /translation="MGTPTSAARPGAAVPDFLAQVFAQYPLEVVSAEGVWLKNSRGER
                     VLDLYGGHAVVALGYGHPGFTRALADQAQSCNFQTNAVPMQVRRRAAERLVRFSGLPF
                     TSVFFVNSGAEANENALKLAFRLTSRGHIAAVEGAFHGRTAAAGAVTWGAQQKWYCFP
                     RAPFDVSFIPRGEIAAINEHVTPATAAVIVEPVQGLAGAVEMGSAFLAALRRRCDEVG
                     ALLIFDEVQCGMGRVGQPFAAQLYGITPDMLTTAKALGNGFPCAALLAAERVSAALKL
                     ELLGTTFGGGPMACAVMEAVIEAIETDKLLEHVQRVAQYIREQCIVGPVSAHQGAGFL
                     IGLRTTRPAKEVQAELLECGILTGTATDPNILRLLPPFILEEEHVDMLRDTLADLPA"
     gene            complement(3571..4632)
                     /gene="argC"
                     /locus_tag="VFB37_01310"
     CDS             complement(3571..4632)
                     /gene="argC"
                     /locus_tag="VFB37_01310"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZO21109.1"
                     /translation="MSERIPALVLGGTGYVAGELLRLIATHPRFELAAVLSDSQPGEP
                     LAAAFPQLGCVYPDTRFKAQGEIEKLLSELPQAAVFSAAPHGVSAALIDALLSAAARA
                     GKHPRVVDISADFRYASAEAYAAVYQHAHGAPNRIAEFTCSVPEHLSKLETPHVAHPG
                     CFATAILLASVPLLKLGFTAPMFFVAGVTGSTGAGRKPIEGTHHPLRHGDLYSYNALA
                     HRHAPEVAACARAATGIDADFAFVPHSGPFARGIHVTVQAPLRSPNDTAAVLAGLRDF
                     YAAAPFVHVSDLAPRVKDVVASNYAHLSASANGRSVAVTCAIDNLDKGAAGGAVQWMN
                     RMFGLAETTGLTAPAPGWT"
     gene            complement(4629..5696)
                     /locus_tag="VFB37_01315"
     CDS             complement(4629..5696)
                     /locus_tag="VFB37_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21110.1"
                     /translation="MNGMRSIVVDKIASVTQACGLSHEVRVAGEIPAEEGVVVAVEVL
                     TNKSTYNTLELTSGRMAKVGKGDVVVGALGHRKALFGYSGHVPQSVQPGDVIQMLNIG
                     GVLGICDSVNPDKGRPFDCRVIGSVLHFPYLGERIGIPARVGHKRLDLEATLQTGGVP
                     VVALAGTCMEAGKTAAACAVIGRMRHRGLVIDAFKATGVSLRRDILAMEDAGARRSMI
                     FTDLGVVSTTKSTGPALTRTMLTELAAGKPDAIVFELGDGILGTYGVDAILECPDIRA
                     GLSGVILSANDPVAAWGGVKLLRERFGIEPCVVTGPSTDNQVGIEIITKQLGVPACNA
                     LTQGASLGDRVIEAIGLRSLS"
     gene            complement(5702..6937)
                     /gene="argG"
                     /locus_tag="VFB37_01320"
     CDS             complement(5702..6937)
                     /gene="argG"
                     /locus_tag="VFB37_01320"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZO21111.1"
                     /translation="MVETASQSGGMPIVLAYSGGLDTSFLVPWIAENHGRPVITVTVD
                     TGGIDETTARTLAERARALGAVEHHQVDARADYFEQVLRFLIMGNVRRGNLYPLCVGA
                     ERVMQAQTVAHMARELGTNVIAHGCTAAGNDQVRFEVAVRTLAPDLEVLAPVRDRSFR
                     RQEELDYLQKRRLPVPPYGAAYSVNRGLWGVTIGGKETLTSSGSIPESAWVLSRDAFT
                     APRAPERHVISFQRGRPSALDGESLSPVALIESLEAIAGPFGIGRGIHLGDTIIGTKG
                     RVAFEAPAAETLLTAHRELEKLVLTGRQQRIKELVAQPYGDLVHEGQLLDPVCRDIEA
                     LLLSSQERVSGSVHVLFRPGQLFIEGVESPYSLMTASKGVYGEAVGEWTPADALGFSK
                     IVSLTGVFAHRAGAPTERR"
     gene            complement(6943..7395)
                     /locus_tag="VFB37_01325"
     CDS             complement(6943..7395)
                     /locus_tag="VFB37_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21112.1"
                     /translation="MQTEASQLDRRGTIVRLLRAGLIRRQGDLVRLLKKEGHEVTQSS
                     VSRDLRDLGVLKASGRYVLPPDEVTRANGDFGMLAQFVRGMRRAGHSITVLRTTIGAA
                     QSVAVAIDKAEWPEVAGTISGDDTIFIATATARAQDELVERLRSLFRI"
     gene            7565..8275
                     /locus_tag="VFB37_01330"
     CDS             7565..8275
                     /locus_tag="VFB37_01330"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HZO21113.1"
                     /translation="MDRWHAAAGRRYAPALWLMVLSALPLIVLGASRDPFTSKDASGA
                     LRTALVQGVDEAVNRLGSPDGFLQNPQYTIPLPPALVKADRPLRLIGMSGDVEKLKTA
                     MNHAAEMAVANARPLFKRAVLQMPIKDAKGLLRGGDTAATSYFRKAMGLELARNFKHI
                     VARATVRANARQLYDEYAAKAAQVGFISSEDGSLNDYLTASAMAAVFEEIGTQETAIR
                     KDPASQKSALIRKVFGAL"
     gene            complement(8296..9054)
                     /locus_tag="VFB37_01335"
     CDS             complement(8296..9054)
                     /locus_tag="VFB37_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21114.1"
                     /translation="MWLEREKERHHNRTSSRANATQFVSGPEWPQAWSDGMTQREHAL
                     WETPWPCAESLRALAELNEQCLELLTKQVLQHAAPAAPLLRELGDLWSRLDAPARRRA
                     AACPYLLLDPGFTDLYRWRWVAGSQVEDREPAFPSFFSVPGVTGLAHQVFSNAWYMVR
                     TQPLGVPLFLGMSAPCGAVFRACSLRQITDLAERHAAWLKPRWAGRPRIWSELLRAAI
                     SGEGLALEKARMRGINLLAAELKLLEQPHGLERR"
     gene            9257..9412
                     /locus_tag="VFB37_01340"
     CDS             9257..9412
                     /locus_tag="VFB37_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21115.1"
                     /translation="MLEVPLMMAAIYFILKVLAEHIAGKLLAAGLHYAFCAGTAPWAT
                     PAVCAVL"
     gene            complement(9466..>9944)
                     /locus_tag="VFB37_01345"
     CDS             complement(9466..>9944)
                     /locus_tag="VFB37_01345"
                     /inference="COORDINATES: protein motif:HMM:NF039696.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6236 family protein"
                     /protein_id="HZO21116.1"
                     /translation="FSFYNSLPVPAPDVPYAEILDLKARRGPELHALREHLEKLYLSV
                     INSNDIFRSQIVALRDLDASIATLARLMVESKLECLWQTLSVELLMEAGVGTATGAEL
                     AELGRQPVAYGAAAGLAGALYVFAKKRLKSVVSRAGPLAYLVHATHEEVIGSVRAP"
BASE COUNT         1790 a         3294 c         3147 g         1713 t
ORIGIN      
        1 cggtgtgcta gtccctcgag ggcctctgcg acctgcaccg ctctcccgct cagctccttc
       61 gtggcatcgc gcaggtagag gcgaaccgcc gcgagcacct gatcattgcg cgaccgcccc
      121 agatgaacac gcgctcccgc ggcgccgatg cgtgcggtca gccgccgctc gagtgcggtc
      181 tgtccatcct cgtcctgaag ctcgatctgc cagagtccgc gcgtgtgctc cagcgccagg
      241 tgtgcaagcc catcgcgaat ggcgtgcagg tcctctgccg agatcagctt ctgggcatac
      301 aacatctcga cgtgcgcgat cgaggcctgc acgtcatatc gcacgagacg ctcgtccaac
      361 gtgtgatcct ccccagccgt gtagcgcaac acgctgtcgc tcagctcggt gcctttgtcc
      421 cagaggcgcg tcatgggatg tcggtcgaac cttgcgccag gtgctgctcg gccagcgtca
      481 aggcgttgat atcggcgacg atcagtgtac cggtgccctc gttggtgaag acttcgccca
      541 ggatcccttc gggggactta tatgagatga catggacccg gcgcacgccg ccgcggatcg
      601 cctcctcgat ggccttcgcc ttgggaagca tgccctcgag gaggcggcct tcatccttca
      661 agcgcttcag cccgcgcaag tccgtgtacg agatgagaga gccggcatcc cccacacgct
      721 ccaggatccc gggagctcca gtgcagagaa tgagcttctc tgctcccagg gcggccccga
      781 tggcggcagc cacggtatcg gcattgatgt tgagaagcgt accgctctga tccgccgaga
      841 ggggactgac gacgggcatc aacccgttgt cgagcagctt gcggatgaca gtcggatcga
      901 ccgcgtcgat atcgccgacg aagccgtaat cgaccgtctc gccggtgctg ccgatccgga
      961 ccggggcgcg cttgtgagcc ctgaccagcc ctgcatcgac accgctgatg ccgaccgcgt
     1021 cgatgttcag atcccggcag attcccagaa tgcgggtgtt gatcaggccg ttgagcacca
     1081 tcgcggtgac atcgatcgac ttctgatcgg tgacgcgccg gccttccacc attcgcgtcg
     1141 gaaccccgag cgcctcggag agatcggtga gctgcggacc gcctccgtgc accacgacaa
     1201 cgcgaacgcc gaagtaatgc aggattgcaa tctgctctat gagaacacgc gtcgtcgccg
     1261 catcggcgaa caccccgccg cctgacttca cgatgaaggt tttccccttg tacatgcgaa
     1321 tgtacggcgc ggcgctcttc agcgcacgga tggcgagcgc ctgctcgccg cgatgcatga
     1381 tcattgcggg tcatcctctc ttgagcattt gatacagcac cgccatctgc acgatgaggc
     1441 ggttgaaggc ctggcgctgc acgatactgc gcggcccatc cagcacttca tccgccacgg
     1501 ccgtgttgcg tcggacaggc aggcagtgca tcaagcgaca gtcggcggcc gccggcgcaa
     1561 accactcatc ccggacgcac cagtcggtga gctccgcgcg caaccgtgca tccgccagag
     1621 tgtcgccata gcagctcgtc gcgccccact ccttggcata gaccacgctg gctcccgtca
     1681 gcgcctcgcc gcggtccgag gtctcgcgga ccgatccgcc ggacagctcc gcggcccggc
     1741 gcgctttgtc catgattacg ggcggcagct cgaagcctgc aggccgcagc acgatgacct
     1801 ccatgccgcg catggcaccc atgtggaggg ccgcggccgg gaccgccagc ggcagcgcgc
     1861 gtgggtgata gacccacgac aatacgaaac gactctgtcg cgggataccc agatcatcga
     1921 gggtcttcca gtccgcgagt gcctggcagg gatggttggc ggcggactcg aggttgatga
     1981 gcggcttgtc caccagctct gccatcgact tgaagttcga ctcggccaga tcggctgcca
     2041 ggtccttacc ctcggcgaag gcccggattc cgagggcgtc gcagtacgat gccagcaccg
     2101 ggatgccttc gcgaacatgc tcggcggccg cgccgttcat gacgacaccg gtccgcgtct
     2161 cgagctgcca ggtgccttgc cctggcgtga tgacgaacga gctgccgccc agacgggcca
     2221 tgcccgactg gaacgaagcc agggttcgca gcgagggatt gaagaacagc agccccagca
     2281 ccttgcccga caacgcctgc ggctccggcc ggatctcgag acgccgcgca agctcgagca
     2341 gcgagccgag ctcctcccga gaaaaatccc ccaggtcgag aaagcgcttc acgcgggaag
     2401 atccgccagc gtgtcgcgca gcatgtccac gtgttcctcc tcgaggatga acggtggcag
     2461 caggcgcaga atgttgggat ccgtggccgt gccggtgagg attccgcatt ccaggagctc
     2521 ggcctggacc tccttcgccg gacgggtcgt gcggaggccg atgagaaacc cggcaccctg
     2581 atgtgcactc acagggccca cgatgcactg ttcgcggatg tactgagcga cgcgctgcac
     2641 gtgttccagc agcttgtcgg tctcgatggc ctcgatgacg gcctccatga ccgcacacgc
     2701 catcggcccg ccgccgaagg tcgtcccgag cagctccagt ttcagagcgg cggatacgcg
     2761 ctccgctgcg agcaacgccg cacacggaaa tccattcccg agagccttcg ccgtggtcag
     2821 catgtcgggt gtgatgccgt acagctgagc ggcgaaaggc tggcccacgc ggcccatgcc
     2881 acactgaacc tcgtcgaaga tcagcaatgc gcctacctca tcgcaacgac gacgcagcgc
     2941 ggcgaggaag gcgctgccca tttcgaccgc cccggcgagc ccctgcaccg gctcgacgat
     3001 caccgccgcc gtcgcgggcg tcacgtgctc attgatggca gcaatctcac cgcgagggat
     3061 gaaactgaca tcgaagggag cgcgcgggaa gcagtaccat ttttgctgcg cgccccacgt
     3121 gacagcgcct gctgcagccg ttcggccgtg gaaggcgccc tcgaccgctg caatatggcc
     3181 gcgagaggtg aggcgaaagg ccagcttgag cgcattctca ttggcctcgg caccgctgtt
     3241 gacgaagaac acgctcgtga acggcagtcc ggagaagcgc acgagccgct ctgcggcgcg
     3301 ccgccggacc tgcatcggga cggcgttcgt ctggaagttg catgactgcg cctgatcggc
     3361 cagtgcgcgc gtgaagccgg gatggccgta accaagcgca acgacggcgt ggccaccgta
     3421 caagtccaaa acgcgctcac cgcggctgtt cttgagccac accccctctg cggacacgac
     3481 ttcgagcggg tactgcgcga agacttgcgc caggaaatcc ggaactgccg cgcccggtcg
     3541 cgctgctgag gtgggggtgc ccatgccgtg tcaggtccac cccggcgcgg gggcagtcag
     3601 gcccgtggtt tcggccaggc cgaacatccg gttcatccac tgcaccgcac cgccagcggc
     3661 gcccttgtcg aggttgtcga ttgcacacgt gacggcgacc gagcggccgt tcgcgctcgc
     3721 agagagatgg gcatagttgc tcgcgactac atccttgacc cgtggcgcca gatcactcac
     3781 gtgaacgaat ggtgctgcgg cgtagaaatc gcgtagtcct gcgagaaccg cggccgtgtc
     3841 gttgggcgag cgcagaggcg cctgcacggt cacatgaata ccgcgggcaa acggcccaga
     3901 gtgagggacg aaggcaaaat cggcgtcgat gcccgtagcc gcgcgggcgc aagcagcgac
     3961 ttccggggca tgacgatggg ccagcgcatt gtatgaatac aggtccccgt gcctgagagg
     4021 atggtgcgtg ccctcgatcg gtttgcgtcc cgcacccgtg ctgccagtca cgccagccac
     4081 gaaaaacatc ggcgccgtga aaccgagctt gagcaacggc acgctggcaa gaagaatcgc
     4141 cgttgcgaag cagccgggat gggcgacgtg aggggtctcg agcttcgaga ggtgctcggg
     4201 caccgagcag gtgaactccg caatccgatt cggcgcaccg tgtgcgtgct ggtacaccgc
     4261 tgcgtacgct tccgccgagg catagcggaa atcagccgag atatccacga ccctgggatg
     4321 tttgcccgct cgcgcagcag ccgagagcaa cgcatcgatc agcgcagcgg acacgccgtg
     4381 cggtgccgcc gagaacacgg cggcctgcgg cagctcgctc aacagcttct cgatctcccc
     4441 ctgggctttg aagcgcgtgt ccgggtacac gcaccccagt tgcggaaagg ccgccgcgag
     4501 cggttcgccc ggctgactgt ccgacaggac ggccgcgagc tcgaaacgcg gatgagtggc
     4561 gatcaggcgc agcagctccc ccgccacata acccgtgccc cccaggacga gggctggaat
     4621 tctttcgctc atgagagcga ccgcaggccg atcgcctcga tgactcgatc acccagtgag
     4681 gcgccctgag tcagcgcgtt gcaggcgggt acgccgagct gcttcgtgat gatctcgatg
     4741 cccacctggt tgtccgtcga gggtccggtg acgacgcagg gctcgatgcc gaaacgctcg
     4801 cgcagcagtt tgacgcctcc ccaggcggcc acaggatcgt tcgccgagag aatcacgccc
     4861 gacaggcctg cgcggatgtc cgggcactcg aggatggcat ccactccgta tgtccccagg
     4921 atgccgtcgc ccagctcgaa cacgatggca tcgggcttgc ctgcggcgag ctcggtgagc
     4981 atcgtgcgcg tgagagcagg tccggtgctc ttcgtggtgg agacgacacc caggtccgtg
     5041 aagatcatcg agcgccttgc gcccgcatct tccattgcca ggatgtcccg ccgcagcgat
     5101 acgccagtgg ctttgaatgc gtcaatcacg aggccgcggt ggcgcatgcg gccgatgact
     5161 gcgcaggccg cggcggtctt gcctgcctcc atgcaggtgc ccgccagtgc gacgacgggg
     5221 acgccacctg tctggagggt ggcttcgaga tcgagccgtt tgtgcccgac ccgcgccgga
     5281 atgccgatcc gctcgccaag atagggaaaa tgcagaacgc tgccgatgac gcggcagtcg
     5341 aaaggcctgc ccttatcggg gttgaccgag tcgcagattc ccagcacgcc cccgatgttg
     5401 agcatctgga tgacatcgcc cggctgcacg ctctgcggca cgtgcccgga atagccaaag
     5461 agggctttgc gatgacccag tgctccgacc accacgtcgc ccttgccgac tttggccatg
     5521 cgcccactgg tgagctcgag ggtgttgtaa gtggacttgt tggtcagcac ttccacagcg
     5581 accaccacgc cttcttcggc cgggatctcg ccggccacgc ggacctcgtg cgacagcccg
     5641 caggcctgcg tcaccgaggc aatcttgtcc acgacgatgc ttcgcatgcc attcatgccc
     5701 gtcatctccg ctcggtgggc gcgcctgccc gatgagcaaa cacgccggtc agggagacga
     5761 tcttcgagaa acccagagcg tccgctggcg tccactcacc gaccgcttcg ccatacacac
     5821 ctttggaggc ggtcatgagg gagtaagggg actcgactcc ctcgatgaac agttggcccg
     5881 ggcgaaacag cacgtgcaca cttccgctga ctctctcctg ggaagacagc agcaaagctt
     5941 cgatatcgcg acagaccgga tccagaagct ggccttcatg caccaggtcg ccgtagggct
     6001 gcgcgacgag ctctttgatg cgctgctgtc ggccggtcag cacgagcttc tcgagctcac
     6061 ggtgcgctgt cagcagcgtc tcggccgccg gggcctcgaa cgctacccgc cctttggtcc
     6121 cgatgatggt atcgcccaga tggatccccc ggccgattcc gaacgggccg gcgatcgcct
     6181 ccagcgactc gatcagggcc accggcgaca gcgattcgcc atccagagcc gagggccggc
     6241 cgcgctggaa agaaatgaca tgccgttcgg gcgctcttgg agcggtgaat gcatctcgcg
     6301 acagaaccca ggcgctctcg ggaatgctgc cgctggaggt cagcgtctcc ttgccaccga
     6361 tggtgacgcc ccacagcccg cgattgacag agtaagccgc gccatacggc gggaccggca
     6421 accggcgctt ctgcagatag tcgagctcct cctgacgccg gaagctgcgg tcacgcaccg
     6481 gggccagcac ctccagatcc ggtgccagcg tgcgcacagc aacctcgaac cttacctggt
     6541 cgttgcctgc agccgtgcaa ccgtgagcga tcacgttggt gccgagctcg cgtgccatat
     6601 gcgcgacggt ctgcgcttgc atcacccgct ccgctccgac acacagcggg tacaggtttc
     6661 cccgcctgac attgcccatg atcaggaagc gcagcacctg ttcgaaataa tcggcgcgcg
     6721 cgtccacctg atggtgctcc accgccccga gcgctcgggc gcgctccgcc agcgtgcgcg
     6781 cggtggtctc atctatcccg ccggtgtcca cggtcacggt gatgactggc cggccgtggt
     6841 tctccgctat ccacgggacc aggaaagacg tatcgagtcc gccggaatag gcgaggacga
     6901 tgggcatgcc gcccgattga gaggccgtct cgaccatgag ctctagatcc tgaataaaga
     6961 gcgcagacgc tcgaccagct cgtcctgcgc gcgcgcggtg gcggtggcaa tgaagatcgt
     7021 gtcatctccc gagatcgtgc ccgcgacttc cggccactcg gccttgtcga tcgcgaccgc
     7081 gacgctctgg gccgcaccga tggtcgttct gagaaccgtg atggagtggc ctgcgcggcg
     7141 catgccgcgc acgaactgag cgagcatgcc gaagtcgccg ttggcgcggg tcacctcatc
     7201 cggcggcagc acgtagcgac cgctcgcttt gagcacgccg agatcgcgca gatcccggct
     7261 gaccgaggac tgcgtgacct catgtccttc tttcttcagc agccgcacga gatccccctg
     7321 ccggcggatg aggccagccc gcaggaggcg gactatggtt ccgcggcgat ccagttgtga
     7381 ggcttccgtc tgcatggcat ttcgcgctgt gcataaattc gcgcgcattc tgcataatta
     7441 tgcagaatcg gtcaagcgtc actgccagcc gggcggcacg cacacccgcg aacgcacgca
     7501 aagcgggtca agcgcagccg ctgcggtacc atcgcgctgg cgaatacttc tagcgagagc
     7561 attcatggat cgatggcatg cggcggccgg gcgtcgatac gcgccggcgc tctggttgat
     7621 ggtgctgagt gcgcttcccc tcatcgtgct gggcgcctcg cgggatccgt tcacctccaa
     7681 ggatgccagc ggcgcgttgc ggacagcgct ggtgcaaggg gtggacgagg cggtcaaccg
     7741 cctcggatcg cccgacggat ttctgcagaa tccgcagtac acgattcctc tgccccctgc
     7801 gctggtgaaa gccgatcggc cgttgcggct gatcggcatg tccggggatg tggagaaact
     7861 caagacagcg atgaatcatg ccgccgaaat ggccgtcgcc aatgcacgtc cactcttcaa
     7921 gcgcgccgtg ctgcagatgc cgatcaagga cgcgaagggg ctcctgaggg gaggggacac
     7981 cgccgccacg agttatttcc gcaaggcgat gggcctcgag ctggctcgca acttcaagca
     8041 catcgtggcg cgtgcgaccg tgagggcgaa cgcgcgccag ctttacgatg aatacgccgc
     8101 caaggcggcc caggtgggat tcatcagctc cgaggacggc agcctgaatg actacctgac
     8161 ggcctctgcc atggcagccg tgttcgaaga aatcggtacg caggagacgg cgatccgcaa
     8221 ggatccggcg agtcagaaga gcgccctcat caggaaggtt ttcggagcgt tgtagcggtg
     8281 cctggctttc ggggctcagc gacgctcgag gccgtgcggc tgctcgagta acttcaattc
     8341 cgcggcgagc aggttgatgc cgcgcatgcg tgccttctcg agtgccagac cttcgccaga
     8401 gatcgccgcc cgcagcagct cgctccagat ccgcggccgg ccagcccagc gaggtttcag
     8461 ccatgcggcg tgtctctcgg ccagatccgt gatctggcgc aagctgcacg cacgaaacac
     8521 cgcaccgcaa ggcgcggaca ttccgaggaa caggggcacg ccgagcggct gggtgcggac
     8581 catgtaccac gcgttgctga acacctggtg cgccaggccg gtaacccccg gcacgctgaa
     8641 gaaggaagga aaggcgggct cccggtcctc gacctggctg ccggccaccc agcgccatcg
     8701 atacaggtcg gtgaatccgg gatccagcag caggtagggg caggcggcgg cgcgccgccg
     8761 ggccggggca tcgaggcggc tccacaagtc gccgagctcc cgcaacagcg gcgccgcggg
     8821 agcggcgtgt tgcagcacct gcttcgtcag cagctccagg cactgttcat tcaactccgc
     8881 cagggctcgc aggctctccg cacacggcca cggggtttcc cacagtgcat gttcgcgctg
     8941 cgtcatacca tccgaccagg cttgcggcca ttccggcccg ctcacgaatt gcgtggcgtt
     9001 cgcccggctg ctggttcgat tgtggtgccg ttctttttcg cgttccaacc acatgatggt
     9061 ctccctttcg cgagctatct tcacgcagtt cggcctgcat tgcacgcccg aatttggtca
     9121 aattatccca atataacgca taattcattg atttccatgg gattaatacc cgaatttctg
     9181 cagttatacc gtctagcgta ccgacacgcg cgtttgcagt cgtaccgcgc acgcttcttg
     9241 cttagcggat ggcctgttgc tggaggtccc cctcatgatg gccgccatct atttcattct
     9301 gaaggtcctc gctgagcaca tcgccgggaa actgttggct gccggcctgc actacgcgtt
     9361 ctgtgcaggt acagcacctt gggccacgcc ggccgtctgc gccgttctct gaagcggacg
     9421 ggcgcagcgg aggtggccct ggcggaattg atagaggccg caccttcagg gcgcccgcac
     9481 gctcccaatg acttcctcat gggtcgcgtg caccaaatag gccagcgggc cagctctgct
     9541 cacgaccgac ttcaaacgct tctttgcgaa cacatacagt gcgccggcca gccccgcggc
     9601 ggcgccgtac gcaactggct gccggcccag ctctgccagt tctgcgccag tcgcggtgcc
     9661 tacgccggcc tccatgagca gctcgacgct aagggtctgc cacagacact ccaactttga
     9721 ttcgaccatg agccttgcga gcgtcgcgat cgaagcatcg agatccctca gagcgacgat
     9781 ctgcgacctg aagatatcgt tcgaattgat tactgagagg tagagctttt cgaggtgctc
     9841 tcgaagggca tgcaattcag ggccgcgtcg agccttcagg tcgaggattt ccgcgtaggg
     9901 cacatctggc gcgggtaccg gtaacgaatt gtagaagctg aatt
//
LOCUS       DASRHN010000036         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_339878, whole genome shotgun sequence.
ACCESSION   DASRHN010000036 DASRHN010000000
VERSION     DASRHN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339878"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VFB37_01350"
     CDS             complement(<1..1253)
                     /locus_tag="VFB37_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21117.1"
                     /translation="MAAQAGVAAQADVVGGPMRFEGFLKLTPRMSAIADARGALHSVA
                     HARTTPRALLAADEWQELESIAQWCAERIARQPDARILLVVPGAPGTRERLATLIRQA
                     VDPRRWLGTAADDRRSVAAGPHPQSTDSLVAVEGGKPLITVPAIQHALSTLLWLSGTR
                     GEFQEVSEWLRAPYGPLERAGSRGRLDLWLRDLGRMRLSLHELAAVLRDAPPECSESA
                     RELQAQFAVASQTLSTGAASPRDWSQRFREVLNAFGWPGDRPRGSADQQNVVRFHELL
                     DEFGQLAAASRSMTRDQAVQCLADLAARTAFQPADDDPSVTISPILAAPVVQYDALWV
                     AGLHSEAFPQPVQPDPFLPLGAQVAKGIPAASAAGRLAEAQALLQAWRASTDELMLST
                     AVRSEDLELLPSPLLRPWLDAGAER"
     gene            complement(1821..3116)
                     /locus_tag="VFB37_01355"
     CDS             complement(1821..3116)
                     /locus_tag="VFB37_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO21118.1"
                     /translation="MKTESHVRPSESLRHVRYEIRGRLARRAHELERQGYEIALLNIG
                     NPKAFGFRTPETMRLAMIENLAEAEGYCHQKGIFPAREAIVMQQQARGVTGVTAEEVF
                     LGNGVSELIDLVLRALLNDGDEVLVPSPDYPLWTAAVTLNRGRAVHYPCRPENNFVPD
                     PQEIEKLITSRTRAIVVINPNNPTGAVYPRHVLEALVSVAERHSLIVLCDEIYDQMTY
                     DGIEHVPMATLVTDTLCCTMSGLSKVYRACGYRVGWAVFSGRTHGASDYFNALELLSS
                     LRLCSNAVGQWAVQTALGGYQSIKELISPGGRLYESRRAIVSAAKLSRFLKLEPPAGS
                     MYAFIGVDKKALPDFDDQQFALDLLEKKHVLIVPGVSFNVPYRDHFRVTTLPDSRLLQ
                     DVFGRIEELLIEYANTPTKSTAPTGNAQDNVVEARGRFK"
     gene            complement(3250..>4268)
                     /locus_tag="VFB37_01360"
     CDS             complement(3250..>4268)
                     /locus_tag="VFB37_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZO21119.1"
                     /translation="GPGEMPASAAAVAKEPPLSVSRLSVSFPVRRGLLGKRAELRAVD
                     DVSFELRAGEAVGVVGESGSGKSTLARAVLQLIHPQGGQVVWMGREVAGLSSHQLKPL
                     RRDLQIVFQDPLASLDPRMSVGEAIAEPLLVHRSDLRPGELSAAVMEALRNVGLGSEM
                     ATRFPHELSGGQCQRVGIARAMVLKPSVLICDEPVSALDVSVQAQIVVLLQNLKRQSG
                     TSLLFVSHNLAVVRRLCDRILVLYMGRMMELAAASQLYTRPRHPYSRALLDAVPVSDP
                     DIQPGRFARSILGEPPSPLAPPSGCVYRTRCPHAIEVCRDQRPSWEPTGAEGEVACHR
                     WREL"
BASE COUNT          765 a         1448 c         1339 g          716 t
ORIGIN      
        1 tgacgctcag cgccagcgtc gagccacggc cgcagcagcg ggctcggcaa cagctccaga
       61 tcttctgacc ttaccgccgt gctcagcatc agctcgtcgg tgctcgcccg ccaggcctgc
      121 aggagcgcct gggcctccgc gagccgcccg gcggcgctgg cagccgggat tcctttggcg
      181 acctgcgcgc ccaggggcag aaacggatcg ggctggaccg gttggggaaa cgcctctgaa
      241 tgcagcccgg ctacccacag tgcgtcgtat tgcaccacgg gcgcggccag gatcggcgaa
      301 atcgtcacgg acggatcgtc gtccgctggc tggaacgccg tccgtgccgc aaggtcggcg
      361 aggcattgca cagcctgatc gcgtgtcatc gagcgcgatg ccgcggcgag ctggccgaac
      421 tcgtcaagca gttcgtgaaa gcgcacgacg ttctgctgat cggcactccc acgaggacga
      481 tcaccgggcc agccaaatgc attcaggact tcgcggaatc gctgcgacca gtcgcgtggc
      541 gaagccgccc ctgtagacag tgtttgcgac gcaacggcaa actgcgcctg taactcgcgc
      601 gcggattccg agcactcggg tggggcgtcg cgcagtacgg cagcaagctc gtgcaaactg
      661 agccgcatac gtcccaaatc acgcagccac aaatccaaac ggccacggga ccccgcacgc
      721 tccagcggcc catacggtgc gcgcagccac tcgctgacct cctggaattc gccccgcgtt
      781 ccgctcagcc acaggagcgt cgagagtgcg tgttgaatag ccggcacggt gatcagcggc
      841 ttgccgccct ccaccgcgac gagcgagtcg gtggactgtg gatgcggacc ggctgcgacg
      901 gagcggcggt cgtcagccgc agtgcccagc cagcgccgcg ggtccacggc ctgacggatc
      961 agcgtcgcca agcgctcgcg cgtcccgggc gcaccgggaa ccaccaaaag aatccgcgca
     1021 tcaggctgcc tggcaatgcg ctcggcgcac cattgggcga tggattcgag ttcttgccac
     1081 tcgtccgcgg caagcagcgc tcgtggggtc gtccgcgcat gcgcgacgct gtgtaatgct
     1141 cctcgggcgt cggcgatcgc gctcatccgc ggggtcagct ttagaaagcc ctcaaagcgc
     1201 atcggcccac cgaccacgtc cgcctgcgct gccacccccg cctgcgccgc caccgccgac
     1261 tgcgctccca accccgcctg cgctgttggc cccgcgtgcg cccccggcgt cagcgcaccc
     1321 gcaagcgccg atagactcgc ggctccgagc gcgcgacaac gctcatccag tgcccgatgc
     1381 acatcacgaa gcaatgtagg ttctgtaccg cgagcaccgc cgatctttct cgtgtcgatg
     1441 tcgaactccg ccgcaagcgc gctcgatcgc cgcagtcctt ccgacagcgc gccacggttc
     1501 accagatcga ggcccgtcgt tgcctccgcc gtgcattgac gccagaggag ccagtcctcg
     1561 gccggggtta gcaggcgagg cagtttcgtg acggttgaaa ggcgctcaat ctctctggtg
     1621 agccaggcat cgagagagag aacgtcaggc gacttccaga ccctgcgtcc agcgctcatc
     1681 tgcgccgcgg cgtgcgcgag tcgagctgca tgcgcacgct gacgcgaggg gacgaccagc
     1741 gtcccgcctc gttgaatgag ctcgcttaac tcagtggaaa gcctaatcaa aacagtcgat
     1801 tgcgagcatg atctcgtgtt ctacttgaac cggccgcgcg cttcaacgac gttgtcctga
     1861 gcattacccg taggtgcggt ggacttcgtc ggcgtgttgg cgtactcgat caggagctcc
     1921 tcgatgcggc cgaacacatc ctgcaggagt cgcgaatcgg gcaacgtggt cacccggaag
     1981 tgatcccgat agggcacgtt gaagctgacg cccggaacga tcaggacgtg cttcttctcg
     2041 agcagatcga gcgcgaactg ttgatcatcg aagtccggca gcgccttctt gtccacgccg
     2101 atgaatgcgt acattgagcc cgcgggcggc tcgagcttca ggaatcggct taacttggcc
     2161 gcgctcacga tcgctcgtcg cgattcatag aggcggcccc ccggcgaaat gagttccttg
     2221 atgctctgat agccccccag cgcggtttgc accgcccact gaccgacagc gttgctgcac
     2281 aagcgcaggg agctcagcag ctccagcgca ttgaaataat cgctcgcgcc atgggttcgg
     2341 cccgagaaca cggcccaacc gacccggtaa ccgcaagcgc ggtagacctt ggacaatccg
     2401 gacatcgtgc agcacaacgt atcggtaacg agcgtcgcca tcggcacgtg ctcgatgccg
     2461 tcgtacgtca tctgatcgta aatctcatcg cagagcacga tcaggctgtg ccgctccgct
     2521 acgcttacga gcgcttcgag gacgtgtcgc gggtacaccg cgcccgtcgg gttgttcggg
     2581 ttgatgacga cgatcgcgcg tgtgcgcgaa gtgatgagct tttcgatctc ttgcggatcc
     2641 ggaacgaagt tgttctcggg gcggcaggga tagtgaaccg cgcgtcctcg attaagggtg
     2701 accgccgcgg tccagagcgg atagtcaggg ctcgggacga gcacttcgtc cccgtcgttc
     2761 agcaacgcac gcagcacgag gtcgatgagc tcactgacac cgttgccgag aaatacctcc
     2821 tcggccgtga ctccggtaac accacgcgcc tgctgctgca tcacgatcgc ttcgcgcgcg
     2881 gggaagattc ccttctggtg gcagtagccc tccgcctcgg cgaggttctc gatcatcgcc
     2941 agccgcatgg tctccggggt gcgaaacccg aatgccttcg ggttgccgat gttcaacagc
     3001 gcgatctcat agccctgccg ctcgagctca tgagcccggc gggccaggcg gccgcggatc
     3061 tcatagcgaa cgtggcgaag agactcgctc gggcggacat gcgattcagt tttcattggc
     3121 cggctttgca ggtcgaaagt gcgtcagtac gggccccctg agcatatcaa ggagacggtg
     3181 ccgcgtcccc ggcggctccg ccagcctgag atccagtgcc gtcacggcgc gcggctctca
     3241 tttgcggctc tacagctctc gccacctgtg gcaggccacc tctccctcgg cccctgtggg
     3301 ctcccaggag ggccgctgat ccctgcacac ctcgatggcg tgggggcacc gggtccggta
     3361 cacgcagccg ctcgggggcg ccagcggcga cggcggttca cccagaatgg atcgggcgaa
     3421 gcgtccaggc tggatgtcgg ggtccgagac gggcacggcg tccaggaggg ctcgggagta
     3481 tgggtgtcgc ggccgcgtgt acagctggga cgccgccgcc agttccatca tgcgtcccat
     3541 gtacagcacc agaatcctgt cacacaggcg tctgacaaca gcgaggttat ggctgacgaa
     3601 caggagcgag gtgcccgact gccgtttgag gttctgcagc agcacaacga tctgcgcctg
     3661 tactgagacg tccagagcgc ttaccggctc atcacaaatc aggacgcttg gtttcagcac
     3721 catcgcgcga gcgatgccga cgcgctgaca ctggcctcca ctcaactcgt gagggaaacg
     3781 agtcgccatc tccgagccca ggccgacgtt gcgcagggcc tccatcacgg cggcgctcag
     3841 ttcccctggc cttagatcgc tgcgatgaac gagcaacggt tcagcgatcg cctcgcccac
     3901 gctcatccgt gggtcgaggc tcgcgagagg atcctgaaaa acgatctgca ggtcgcggcg
     3961 cagcggtttc agttggtgcg acgacaggcc cgccacctcg cgccccatcc acacaacctg
     4021 cccgccttgg ggatgaatga gctgcagcac cgcccgagcg agggtcgact tccctgaccc
     4081 ggactcccca acgacaccaa ccgcctcccc cgcgcgcaat tcgaaggaaa catcgtcgac
     4141 ggcccgcaat tcggcccttt tacccaacag cccgcgtcgc acgggaaagc tgacactgag
     4201 gcgcgagacg ctcagcggtg gctccttggc caccgcggcg gcgcttgcgg gcatctcgcc
     4261 cggtcccg
//
LOCUS       DASRHN010000037         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1165804, whole genome shotgun sequence.
ACCESSION   DASRHN010000037 DASRHN010000000
VERSION     DASRHN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165804"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /locus_tag="VFB37_01365"
     CDS             <1..2112
                     /locus_tag="VFB37_01365"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HZO21120.1"
                     /translation="YQEHLAKTAPHVLKFSFSLVMDGRRLPRPVNYVLVRMIPPDDTE
                     IDPRKRPFVVVDPRAGHGPGIGGFKADSEIGVVFRGGHPCYLIGFLPEPMPEQTIEDI
                     AHAEAAFLEQVIALHPEAEGKPAVIGNCQAGWAVMLLAAMRPGLFGPIIVAGSPLSYW
                     AGVHGRNPMRYTGGLLGGSWLTALMGDLGNGKFDGAWLVNNFENLNPANTYWTKHYNL
                     YRKIDTEAPRYLEFEQWWGGHVLMNADEMQFIVDELFVGNRLASAEIVARDGTRIDLR
                     NIRSPIVVFCSKADNITPPQQALGWVLDLYDCVDDIRAHGQTIVYAVHESIGHLGIFV
                     SSSVARKEHDEFTGNIDLIDVLPPGLYEAVITPKDPTDASADLVGGDYLVRFETRTLD
                     DVRALGGNSEEDERKFATAARLSEINLGLYRTCLQPCVRAWANEGFARWMRRLHPLRL
                     QYEMFSHANPFMRPLLGAAESVLADRRPVASGNVFWQAQEQFAQYIEQTLDAYRDVRD
                     HMTEAWFHALYGSPLLHAMVGLGASDRSVRRRPGKDPAHAAAVARRIQELRSAIAEGG
                     PREAVLRALLYIRMPEGLVDERGFNLLRRAREEAGDGLTLGEFKQLMREQFLTLLLDE
                     RRAVEAIPAMLAKDRDLASRMATNLRRMIDVVGLHTGLAKARLAEIEELIEGSRSARY
                     EKRAVTGVGAAKSQAAASPKH"
     gene            2158..2586
                     /locus_tag="VFB37_01370"
     CDS             2158..2586
                     /locus_tag="VFB37_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21121.1"
                     /translation="MAGPLRPTELKQISAKAEIAKMDEERQFARKKEQHAAELREAFM
                     ARDVLPDAIDRVNRAVRVAAEQGLHQIQVVTFPVKYCNDGGRSINNFDPEWPRSLEGY
                     AKRAFAFYEKELRPLGYKVSAEVISFPDGIPGEAALYLKW"
     gene            2650..>3213
                     /locus_tag="VFB37_01375"
     CDS             2650..>3213
                     /locus_tag="VFB37_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZO21122.1"
                     /translation="MISEVREHAKYERLVAATKGLPGLRTAVAHPCDEASLTGAMEAA
                     EAGIIAPVLVGPEDKIRKLAKSLDLDIGAAQVVDVPHSQAAAEKAVAIVRAGEAELLM
                     KGSLHSDELLAEVTKRDTGLRTGRRISHVFVMDVPSHPQTLFITDAAVNIAPDLTAKR
                     DIIQNAVDLYAGLGLGMPKVAILSAVET"
BASE COUNT          587 a         1067 c         1048 g          511 t
ORIGIN      
        1 taccaggagc acctcgccaa gacggcaccg cacgtattga agttttcctt ttccctcgtc
       61 atggacgggc gcaggctgcc gcggccggtc aactatgttc tggtccggat gatcccgccg
      121 gacgacaccg agatcgatcc gcgcaagcga ccgttcgtcg tcgtcgaccc gcgcgccggc
      181 cacgggcccg gcatcggcgg cttcaaggcg gacagcgaga tcggcgtcgt cttcaggggt
      241 gggcacccct gctacttgat cggcttcctt ccggaaccca tgccggagca gacgatcgag
      301 gacatcgcgc acgcggaggc agcgttcctc gagcaggtga tcgccctgca ccccgaggcg
      361 gagggcaagc ccgcggtcat cggcaactgc caggccggat gggcggtcat gctgttggca
      421 gccatgcgac cggggctctt tggccccatc atcgttgccg gctcgccgct gtcctattgg
      481 gccggcgtcc acggccgcaa cccgatgcgc tacacgggcg gcctgctggg tggcagctgg
      541 ctgaccgcgc tgatgggcga tctgggaaac ggcaagttcg acggcgcatg gctggtcaac
      601 aacttcgaga acctcaaccc ggccaatacc tattggacca agcactacaa cctctatcgc
      661 aagatcgata ccgaggctcc gcgctacctc gagttcgagc agtggtgggg cggccacgtc
      721 ctgatgaatg ccgacgagat gcagttcatc gtcgatgagc tgttcgtcgg caacaggctg
      781 gcgtcagcgg agatcgtcgc gcgcgacggc acacgcatcg acctgcgcaa tatccgctcc
      841 cccatcgtcg tcttctgctc caaggccgac aacatcacgc cgcctcagca ggcattgggc
      901 tgggtcctcg acctctatga ttgcgtcgac gacatccgcg cgcacggaca gaccatcgtc
      961 tacgcagtgc acgaatcgat cggccacctc ggcatcttcg tctcttcgtc agtggcgagg
     1021 aaggagcacg acgagttcac gggcaacatc gatctcatcg acgtgctgcc gccgggtcta
     1081 tacgaggcgg tcatcacacc caaagacccg accgatgctt ccgccgatct cgtcggcggt
     1141 gactatctgg tacgcttcga gacgagaacc ctcgacgacg tccgcgcgct cgggggcaac
     1201 agcgaggagg acgagcgaaa gttcgccacg gcggcgcgat tgtcggagat caatctcggc
     1261 ctctaccgaa cgtgcctgca gccatgcgtg cgagcgtggg ccaacgaggg cttcgccagg
     1321 tggatgcgac ggctccaccc gttgcggctg cagtacgaga tgttctcgca tgccaatccc
     1381 ttcatgcgtc cgctgctcgg cgcggccgag agcgtgctgg ccgaccgtcg acccgtggcg
     1441 agcggcaacg tcttctggca ggcgcaggag cagttcgcgc agtacatcga acagacgctg
     1501 gatgcctatc gcgatgttcg cgaccacatg accgaagcct ggttccacgc gctctacggc
     1561 tcgccgctgc tgcatgccat ggtggggctc ggggcatccg atcgcagcgt gcgccgcagg
     1621 cccggaaaag atccggcaca tgccgctgcc gtggcgcgcc gcatccagga gctgagatcg
     1681 gccatcgccg aaggcggacc gcgcgaagcc gtgcttcgtg ccctcctcta catccgcatg
     1741 ccggagggcc tggtggacga gcgaggcttc aacttgctgc gtcgcgcgcg cgaggaggcc
     1801 ggcgacggcc tcaccctcgg cgagttcaag cagctgatgc gcgagcagtt cctgacgctg
     1861 ctgctcgacg agcggcgcgc ggtggaggcg attcccgcca tgctcgcgaa ggaccgcgat
     1921 cttgcctcgc gcatggcgac caatctgcgc cggatgatcg acgtggttgg cctgcacacc
     1981 ggtctggcca aggcgcgtct ggccgagatc gaggagctca tcgagggttc gaggagcgct
     2041 cggtacgaga agcgcgcggt gaccggcgtc ggcgccgcca agtcccaggc agccgcgagc
     2101 ccgaaacact agtcgcgcct gctacgtgca cacgacctgc gagcagaggt gatcaccatg
     2161 gccggcccat tgcgcccaac cgagctcaag caaatctctg ccaaggccga gattgccaag
     2221 atggacgagg agcggcaatt cgcacgcaag aaggagcagc acgctgccga gctgcgcgag
     2281 gccttcatgg cccgtgacgt gctgcccgac gccatcgatc gcgtcaacag ggcggtgcgc
     2341 gttgctgcgg agcagggcct gcaccagatt caggtggtga ccttccccgt caagtactgc
     2401 aacgacggcg gccgcagcat caacaacttc gaccccgaat ggccgaggtc gctcgagggc
     2461 tacgccaaga gggccttcgc cttctacgag aaggagctgc gtccgctggg ctacaaggtg
     2521 tccgccgagg tcatcagctt tcccgacggc atccctggcg aagccgccct ctacctgaag
     2581 tggtaagcgg ctctgccgcc gcgaccgagc ccacgagcga gaaggcccag tccgatgccg
     2641 agaaaggcct tgatctccga ggtgcgcgag cacgccaaat acgagcgcct cgtggcggcc
     2701 accaaggggt tgccgggcct ccggacggca gtcgcacacc cctgcgacga ggcctcgctg
     2761 acgggcgcga tggaggcggc cgaagccggg atcattgcgc ccgtgctggt ggggcccgag
     2821 gacaagatcc gcaagcttgc caaatcgctc gatctcgaca tcggcgccgc ccaagtcgtc
     2881 gacgttcccc acagccaggc agcagccgaa aaggcagtgg cgatcgttcg cgccggcgag
     2941 gcggagctgc tgatgaaggg aagcctgcat tccgacgagc tgctggcaga ggtcacgaag
     3001 cgggacacgg ggcttcgcac cgggcggcgc atcagccacg tgttcgtgat ggacgttcca
     3061 agccatccgc agacgctgtt catcaccgac gctgccgtaa acatcgcgcc cgacctgacg
     3121 gccaagcgcg acatcatcca gaacgccgtc gatctctacg cggggctcgg gctcggcatg
     3181 ccgaaggttg caattctgtc ggcggtggaa acg
//
LOCUS       DASRHN010000038         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1000713, whole genome shotgun sequence.
ACCESSION   DASRHN010000038 DASRHN010000000
VERSION     DASRHN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000713"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFB37_01380"
     CDS             <1..253
                     /locus_tag="VFB37_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO21123.1"
                     /translation="AGFNLKPGEHPIIPVMLGDAALAGRMAEQLLKRGVYVIGFSFPV
                     VPKGQARIRTQMSAALTREQLDRALEAFAAVGKQLQVIR"
     gene            250..1290
                     /gene="tdh"
                     /locus_tag="VFB37_01385"
     CDS             250..1290
                     /gene="tdh"
                     /locus_tag="VFB37_01385"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR00692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZO21124.1"
                     /translation="MKALVKKQATPGIWLEDVPEPKVGPNDVLIEIVRTAICGTDMHI
                     YKWDAWAQKTIPVPMAVGHEYCGRIIEMGSEVRGLKIGDRVSGEGHITCGYCRNCRAG
                     RRHLCRNTVGVGVNRPGAFAQYLAIPAVNTFKLADSISDDIAAILDPFGNATHTALAF
                     SVVGEDVLITGAGPIGIMAAAIARFVGARHVVITDVNDFRLDLARQMGASRAINVQRE
                     SLDQTMKDLGMQEGFDIGLEMSGNAAALREMLRTMHHGGSIAILGIPPEETAIDWNQV
                     IFKGLTLKGIYGREMFETWYKMASLLQSGLKLEPLITHHFPIQDFLAGFQTMESGKSG
                     KVILDWSTLQIG"
     gene            complement(1563..>2241)
                     /locus_tag="VFB37_01390"
     CDS             complement(1563..>2241)
                     /locus_tag="VFB37_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZO21125.1"
                     /translation="AAGKPVVVSMSDLAASGGYYISAAADEIWASPATLTGSIGIFAI
                     IPTVDKTLGKVGVTVDGVGTTPLASQMRIDRPLGQEARALLQSEIERGYEVFLERVAA
                     GRKKTRDQVNAIAQGRVWAGEDAKRLGLVDALGSFDDATKAAARRAKLTDYDVEFIEP
                     ELTWAEQLVLQMKSRTVRALVSADARTRSLAQVAAQFDPLRREVERLSRFSEPNRVYA
                     YCFCAAE"
BASE COUNT          404 a          736 c          704 g          397 t
ORIGIN      
        1 ggccggattc aacctcaagc ccggggagca tccgatcatc ccggtgatgc tcggggatgc
       61 ggcgcttgca gggcgaatgg ccgagcaact cctgaagcgg ggcgtctatg tcatcggctt
      121 ctcctttccg gtggtcccga aaggccaggc gcgcattcgt acacagatgt cggcggcgct
      181 gacgcgcgag cagctcgatc gcgcgctgga agcattcgcc gcagtgggta agcagttgca
      241 ggtgatccgg tgaaggcgct cgtcaaaaag caggctacgc ctggtatctg gctggaggat
      301 gtgccggagc ccaaggttgg cccgaacgat gttctgatcg agatcgtccg cacggctatc
      361 tgcggcacgg acatgcacat ctacaagtgg gacgcctggg cgcagaagac catcccggtc
      421 cccatggccg tcggccacga atattgcggt cgcatcatcg agatgggctc ggaggtgcgc
      481 ggcctgaaga tcggcgaccg ggtctcgggc gaagggcaca tcacctgcgg ctattgccgc
      541 aattgccgcg ctgggcgccg ccatctgtgc cgcaacaccg tgggggtcgg cgtcaaccga
      601 cccggagcgt tcgcgcaata tctggcgatc cctgccgtca acaccttcaa gctcgccgat
      661 tcgatcagcg atgacattgc ggcgatcctc gatccgttcg gaaacgcgac ccataccgcg
      721 ctcgccttca gcgtggtcgg cgaggatgtg ctgatcacgg gagccggtcc catcgggatc
      781 atggcggccg cgatcgcacg cttcgtgggg gcccgtcacg ttgtgatcac cgacgtcaac
      841 gatttccggc tggatctggc gcgccagatg ggcgcctcgc gcgcaatcaa tgtgcagcgg
      901 gagtcgctgg accagaccat gaaggacctc ggcatgcagg agggcttcga cattggcctg
      961 gagatgtcgg gcaatgccgc agcgctgcgc gagatgctgc gaaccatgca tcacggcggc
     1021 tcgatcgcca ttctgggcat cccaccggaa gagaccgcca tcgactggaa tcaggtgatc
     1081 ttcaagggcc tcaccctgaa aggcatctac ggccgcgaga tgttcgagac ctggtacaag
     1141 atggcgagct tgctgcagag cgggctcaag ctcgagccgc tcatcacgca tcactttccg
     1201 atccaggatt ttctcgccgg cttccagacc atggagtcgg gcaagtcggg gaaagtgatc
     1261 ctcgattgga gtacgcttca gataggctga aagacccctg cggcgctttc agcccgtgcc
     1321 tagcggcgat ctcgatgctc ggcgccgcgg caaaaatgtg actcttgccg ctcaatcctt
     1381 cgcgtttcac gcccgcgccg gaaagaggcc tgcgggggcc ccacagtcga ttccgactgc
     1441 ccttcactcg ggcgcctcga actcgaacat acgctcaggc gaacttgcgc gtcctcgcgc
     1501 ccgactcggc gtacttcgag atcggggtgc tccttgagtg cgctgacgct gacggcttgc
     1561 cactactccg ccgcgcagaa gcagtacgcg tacacccggt tgggttcgct gaagcggctc
     1621 aagcgctcga cctcgcgccg caaagggtcg aattgcgccg cgacctgagc cagcgagcgc
     1681 gttcgcgcat ctgcggatac cagcgcgcga accgtcctgg acttcatctg cagcaccagc
     1741 tgctccgccc aggtgagctc cggctcgatg aactcgacat cgtagtcggt cagcttggca
     1801 cgccgcgccg cggccttcgt cgcatcgtcg aatgagccca gggcatccac gagccccagc
     1861 cgcttggcat cctcgcccgc ccaaacccgc ccctgggcaa tcgcattgac ctgatcccgt
     1921 gttttcttgc gcccggcagc gacgcgctcg aggaaaacct cgtagccgcg ctcgatctcg
     1981 gactgaagca gcgcgcgcgc ctcctgcccg agtggacgat cgatgcgcat ctgcgaggcc
     2041 aatggagtgg tccccactcc atccacggtg acaccgactt tgccgagcgt cttgtctacc
     2101 gtcggaatga tcgcaaagat cccgatcgag cccgtcaggg tagccgggct ggcccagatc
     2161 tcatccgcgg ccgccgagat gtagtagccg ccggaggcgg ccagatcgct catcgagacc
     2221 accaccggct tgccggccgc a
//
LOCUS       DASRHN010000039        12364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1495855, whole genome shotgun sequence.
ACCESSION   DASRHN010000039 DASRHN010000000
VERSION     DASRHN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12364
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495855"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFB37_01395"
     CDS             <1..231
                     /locus_tag="VFB37_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21126.1"
                     /translation="ASARGSAGDAQQAAHEVIQGNETLRQESNRDATGNRHDMSPVYP
                     AAPAGPTDSGPPPSPPPPAAPEAGPSPNSGPN"
     gene            252..1157
                     /locus_tag="VFB37_01400"
     CDS             252..1157
                     /locus_tag="VFB37_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZO21127.1"
                     /translation="MKTFTLVAASALLLAACATPPQRNEQVEQARSEIDTLSQDPLAQ
                     QAAAHDLDAARVSLQQADAAFQQRQPPAIVDHLAYLARRHAEAGEARVAQAHAQQEMA
                     RAQDERNHILLSSREREAQNAQAQAQSAQAQAQSAQAEAQNARNQALAAQQQLADAQR
                     QLQDLQAKKTDRGMVVTLSDVLFDTGQATLKPGASLALDRLAGYLKSNPQTRVIIEGH
                     TDSRGSDDYNQGLSERRAQAVAMELESRGVPEDQYRTRGLGKSYPVASNDTAAGRQQN
                     RRVEIVFSDASGRFAQGATEPSQQR"
     gene            1338..2273
                     /locus_tag="VFB37_01405"
     CDS             1338..2273
                     /locus_tag="VFB37_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZO21128.1"
                     /translation="MNERFNDDRPGERDGPSRDREGSENEAGQSFHPGNPGFPEHQHE
                     RRLEWSGRHTPGQYERGPQYQRSPYQRDAHFERGAPERGHEPGSQPERMGHGSPSFSP
                     GEQRPWNRSGARFEDRRGHDEGGWSQPLNQRAFGHSPFSRSALTESDPPAYFGTGSQG
                     VGGGPGFTGGRYGHADERLDSPYFQEVGFNRGYYGDVPDQYGEGGGAELRRKQALRRR
                     YPPGPKGYQRSDERLREDISERLMHSYDIDSSEVTVEVLGGKVILEGTVPNRYMKHGI
                     EDLADAAPGVQDIDNRIRVVGAGGAASRAPEVHGG"
     gene            complement(2244..3599)
                     /gene="rpoN"
                     /locus_tag="VFB37_01410"
     CDS             complement(2244..3599)
                     /gene="rpoN"
                     /locus_tag="VFB37_01410"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZO21129.1"
                     /translation="MPNAVMGLRLKQQLTLTPRLQQSVKLLQLSALECVQELHQAIAQ
                     NPFLEEAAESPDTRTQAEEASESSHDDLDFSGGGSGGGGEDTPDWTEWTASPSTLHDS
                     LHEQLLLLGLGERDYALANLVVDALDEDGFLRQPLEEIQRFAPGVEPGEIETALRIVQ
                     TLEPTGIAARSLAECLALQLQALERGTPGRELALEIVQNRLPLMAARDHTRLLDALGC
                     SEEGLREAIELIRSLDPRPGSKVGTFEPRAIVPDVIVRKEKKRWMVSINSAIYPRIRV
                     NQQYADYFRQARDGETALLAQHLQEARWLVRNLEQRFLTIQRVAEAVVARQRNFFDYG
                     DLAMRPLTLREIADELGLHESTVSRATSHKYMATPRGVVAFKRFFSRQLATTSGGSCS
                     ATAIRALLREFIAAEDRRNPLSDVQLTELLADRGVKVARRTVTKYRRSMQLPAVDFRR
                     A"
     gene            3799..5463
                     /locus_tag="VFB37_01415"
     CDS             3799..5463
                     /locus_tag="VFB37_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO21130.1"
                     /translation="MRSIHDDGLGSEPSTASERALALARAQYAAVAQKNDALEVVLAI
                     ARLGYCRIDAGTRAVAANSQFKAEFGLPPDAVPSWPQILDRVGTEQRAAFSEAIERAL
                     REGNDLEWRLQTLWPDGTSQWISLRGRLLSDGAEGGALFVISRNVTVEIATAASEQLR
                     HTQSVEHEQRLRMDAEAANRGKDQFLSMFSHELRSPLNAILGWNRILSLKRAEDPEVA
                     SITARIDHSARAQLKMVNDLLDLGRIETGKLRIELRPMRLAQVAAAALDLARPVAAAK
                     SITITSDFEPGGGALRGDPDRLQQVVSNLLSNALKFTPSGGRISLLLRNRNGFAELTV
                     RDTGQGIDPDLLPHVFDRFRQGDSSSTRHAGGLGLGLALVREIVTLHGGSVSAMSAGP
                     GAGAAFTIRFPARPVWRAAETAAPGATELSRYGDSLRGLSILVVDDESDARAVVAETL
                     RLEGARVTVTDSAGSAYAKVREPDAHFDIIVTDIAMPEEDGYSLVRRLRALQTGRQML
                     AIAVTGYASKSDVAAALDAGFDLHVAKPVDLDAFVPMLRRLANGGR"
     gene            complement(5468..5755)
                     /locus_tag="VFB37_01420"
     CDS             complement(5468..5755)
                     /locus_tag="VFB37_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21131.1"
                     /translation="MDGTQQSAIHQALVTVQQAVTSMTFPSCDQGDVIELIDRIEEQL
                     HARHPNVPMMCTFLNSIARSLRAQPEARDVCLVIEDAIGAAGMPSTWQSGI"
     gene            6032..6523
                     /locus_tag="VFB37_01425"
     CDS             6032..6523
                     /locus_tag="VFB37_01425"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HZO21132.1"
                     /translation="MLELVRDMEYEGSAMRTAETISTLNGLIRVCRGAEARCQSWVAT
                     AQGTALRRRLEARAGEWGRQGDELQALVLLLGGEPARGTSFAALLHRLWSASRAVLLG
                     RSDLPVARALDWTQQQALEWYERALEGYLPERIRRTVSLHARHIAVRCERLGAGSGHF
                     ASP"
     gene            6721..7236
                     /locus_tag="VFB37_01430"
     CDS             6721..7236
                     /locus_tag="VFB37_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HZO21133.1"
                     /translation="MSSSSRIVSTSYRHATVALGELLRITRDGEQGLRMCAIRAESGS
                     LRTLFTAMADRYRSAAAELHELIGRIGGDPSMRCVIPGAARRGWVGLHESLALTDDRA
                     LIDDCERAEDHALEVYRNALDDHLPEFVRQAVLQQFEGLMQDHHRIRLLGREPHPTGA
                     VVVSYGGHTQP"
     gene            7217..8407
                     /locus_tag="VFB37_01435"
     CDS             7217..8407
                     /locus_tag="VFB37_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZO21134.1"
                     /translation="MATPSHRIDFVLLRRPALAAVVALALGMILGPLGLRILQPELVD
                     DGAPIESLSEIALLLCLFCAGLRLRVPLEWRHWRMPGRLATLTLGATVAIAAAVAHLM
                     FGMSLTESLLLGAIVAPTDSVLGSDAGPHPEGDHEGSAFVLAAEGGANNGLATGLVML
                     VLAMMGLSDSDSDALGTISLAALWAIGGGFLVGWLTGAAAARCIMLFDPERKTDFLEE
                     GMVFATASLAYGAALLIHTDGFPAVFAAGVALCHGGRLRRTLRNRPLMPRVLRIAARV
                     ERFAWLAIIVLLGSLVASVEFRARMLVFALLLLLLIRPLAVRLGLGGLAVPEAQWRSI
                     AWFTPRGIGCLYCLSFAINHGLSAPFSRELAGITLVVIVSSLIASGISGLPLRRASPG
                     TVDL"
     gene            8654..10045
                     /locus_tag="VFB37_01440"
     CDS             8654..10045
                     /locus_tag="VFB37_01440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZO21135.1"
                     /translation="MAHALIVDDDADVVDWLQEVARMEGFTVARAHSLRDARIELGRQ
                     RPDVLLTDLRLPDGEGIELVRELEKPEATEVIIVTGHATVDSAVAALRAGASDYLVKP
                     ADLDRVQAVLRHARKTSALQHEIGELREELRRLGRFGRILGSSPRMQVLYDQLSRVAP
                     TSATVILIGESGTGKELAAATVHELSRRRDAAFLPLNCGAVAPQLIESELFGHERGSF
                     TGADRQHKGFFERANGGTIFLDEITEMPMELQVKLLRVLETGTVTRVGGTQTIPSDVR
                     VICATNREPEKAVVDGKLREDLYHRLNVFPIRLPPLRERGTDIEQLAQFFLDELNRNE
                     GTNKTFSRDALVRLYQHAWPGNVRELKNYVQRTFIMADEVIEFEIGNADSAPKAEDTT
                     TITIRVGTPLEEVERRVTMATLAYCGHVKRKAAEILGVSLKTLYNRLETYNGKESLGD
                     NKDEAVNAQDSGH"
     gene            10245..10883
                     /locus_tag="VFB37_01445"
     CDS             10245..10883
                     /locus_tag="VFB37_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21136.1"
                     /translation="MPATRVAVVDDHQLVRLGLKQIIESQPDFAWAGEAKTGREALAL
                     LRSAACDVLLLDLSLPDMSGLDVLRQIKAHHESVATLVLSAYPENQYGLNVLRAGASG
                     FVSKTADDAELCRAIRAAVRGGRYVSPELAEMLVSGMQGAAGEPRHSVLSEREFQIFC
                     KLAEGKSVSEIAAKLFLSVKTVSTYRTRILEKMSMKTNADITYYAIKNNLLQ"
     gene            10887..11336
                     /locus_tag="VFB37_01450"
     CDS             10887..11336
                     /locus_tag="VFB37_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21137.1"
                     /translation="MATAQVHPPDASDSAAAERRILLIEDSAVLTRRLIDLLSEPGRV
                     EVAAQASTQTEALQRLQEAAFDVLVVDIELAEGNGVAVIRHARQVYPPQAQPLIVVLT
                     NYASDFVRDHCFAAGADYFLDKMRDIALLKAIVVDGHSKWAPPPASH"
     gene            complement(11384..12106)
                     /locus_tag="VFB37_01455"
     CDS             complement(11384..12106)
                     /locus_tag="VFB37_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZO21138.1"
                     /translation="MIVEQRDIEVSELERLVEARTHELSAFSTHLQSLAEQEKSQLAR
                     TLHDELGGLLTAAKMDLSWLQSRLSPGQEKPVQDRLAQLGSVLDEAMDLKRRIVEQLR
                     PSLLEHFGLATAIRAHVEATCAQAGLECEVLVEHPCDSLSKDMAIGLFRAVQEGLSNI
                     LRHARCRRVELVLGGNAAQCMVTLTDDGQGFDSSGPQITSSHGLMRMRQRIHALGGSM
                     SVESRAGAGTTLRFGVPLPHES"
BASE COUNT         2184 a         3966 c         4098 g         2116 t
ORIGIN      
        1 gcatcagcgc gcggctccgc cggtgatgcg cagcaggccg cacacgaggt gatccagggg
       61 aacgagacac tccgacagga atccaaccgc gatgcaacgg gaaaccggca cgacatgagc
      121 ccggtctatc ccgcagcgcc agccggtccg accgattcag gtccgccgcc gagcccgccg
      181 ccccctgcgg cgcctgaggc gggaccgtcg cccaactcgg gtcccaactg agcctgcctg
      241 aggaaacaac catgaaaaca tttactctgg tcgccgcctc cgccctcctg ttagccgcct
      301 gcgcgactcc gccgcagcgc aacgagcagg tcgagcaggc gcgaagcgag atcgacacgc
      361 tttcccagga tccactggcc cagcaggccg ccgcgcacga tctggatgca gcgcgagtgt
      421 ccttgcagca ggccgacgcg gcgttccagc agcgacaacc cccggccatc gtcgatcatc
      481 tggcatatct ggcccgccga catgcggaag ccggggaagc acgagtcgcc caggcgcacg
      541 ctcagcagga aatggctcgt gcgcaggatg agcggaatca catcctgctt tccagccgtg
      601 aacgtgaagc acagaacgcg caggcgcagg cacagagcgc ccaggcgcag gcacagagcg
      661 cccaggccga ggcacagaac gcaaggaatc aggctctggc ggcgcagcag cagctcgccg
      721 acgcacagcg gcagctgcag gatctgcagg ccaagaaaac cgatcgcggg atggtggtga
      781 ctctcagcga tgtgctgttc gatacgggcc aggctaccct caagcccggt gcgagccttg
      841 cgctggaccg gctggcgggc tacctgaagt ccaatcccca gacacgggtc atcattgaag
      901 ggcacacgga cagccgcggc tccgatgact acaaccaggg actctccgag cggcgcgccc
      961 aagctgtcgc catggagctc gaatcccgcg gagtgcctga ggaccagtat cgaacccgcg
     1021 gcctgggtaa aagctatccc gtggcttcga acgacacggc cgccggccgg cagcaaaacc
     1081 gacgcgtcga gatcgtcttc agcgatgcga gcggtcgctt tgcgcagggt gcgacggagc
     1141 cttcccagca gcgctagcga tgccttcagc ggcaggcgcc cgccccgcgg cccgctgata
     1201 agtaaggaac cgagcagagg gtccagggat ggacctgcct gtgcccccgt tggagggtcg
     1261 ggccgcgtct tcgcccccag gcgacaggta cggcgattgc acctcttggc tgaaatccta
     1321 ccggcgcgag gttgctcatg aacgaaagat tcaacgacga caggcctggc gagcgcgacg
     1381 gcccctcccg ggaccgggaa ggctcggaaa acgaggctgg gcaaagtttt catcccggca
     1441 atcccggctt cccggagcat cagcacgagc ggcgcctgga gtggtccgga aggcatacgc
     1501 ccggccagta cgagcgcggt ccgcaatacc agcgctcgcc gtatcagcgt gatgcccatt
     1561 tcgaacgcgg tgcgcctgag cgcgggcacg agcccggctc gcagcccgag cgcatggggc
     1621 acggatcgcc gtccttctca cccggcgagc aacggccttg gaacaggtcg ggtgcgcgct
     1681 tcgaggatcg gcgcggacac gatgagggcg gctggtcgca acccctgaat caacgcgcgt
     1741 tcggccattc ccccttcagt cgctccgcgc ttaccgagag cgatccgccg gcatacttcg
     1801 ggaccgggag tcagggtgtt ggaggtggac ctggtttcac gggcggacgc tacgggcacg
     1861 ccgatgagcg attggactcg ccgtactttc aagaggtggg cttcaaccgc ggctactatg
     1921 gtgatgttcc tgatcaatat ggtgagggtg gcggggccga gctgcgccgc aagcaggcgc
     1981 tacgccggcg ttatccgccc ggacccaagg gataccagcg ctcggatgag cggttgcgcg
     2041 aggacatctc ggagcggttg atgcactcct acgacatcga ttccagcgag gtgacggtcg
     2101 aagtgctcgg aggcaaggtg atcctcgaag gtaccgtgcc caatcgatac atgaagcacg
     2161 ggatcgaaga cctggctgat gcagcccccg gggtgcagga tatcgacaac cgcatccgag
     2221 tggtgggcgc cggcggcgcg gcctcacgcg cgccggaagt ccacggcggg tagctgcatc
     2281 gagcgccggt atttcgtgac cgtacggcgt gccaccttga cgccacgatc ggcgagcagc
     2341 tcggtgagct gaacgtcgga cagcggattg cggcgatcct ccgccgcgat gaactcgcgc
     2401 agcagtgcgc ggatcgcggt tgccgaacac gagccgccgc tggtcgtggc aagctggcgc
     2461 gagaagaagc gcttgaaagc caccaccccg cgaggggtgg ccatgtactt gtggctcgtg
     2521 gcgcgcgaca cggtcgactc gtgcaagccg agctcgtcag cgatctcgcg cagcgtcagc
     2581 ggacgcatcg ccaggtcgcc gtagtcgaag aaattccgct gccgcgcgac caccgcctcg
     2641 gccacgcgct gaatggtcag aaaacgctgc tcgagattgc ggaccagcca gcgcgcctcc
     2701 tgcaggtgct gcgcgagcag cgcggtctca ccatcgcgcg cctggcgaaa gtagtccgcg
     2761 tactgctgat tgacacgaat gcgtggatag atcgccgagt tgatactgac catccagcgt
     2821 ttcttttcct tgcgaacgat cacatcaggc acgatggcgc gcggctcgaa cgtcccgacc
     2881 ttggagccgg ggcgcggatc cagactgcga atgagctcga tcgcctcgcg cagcccctct
     2941 tccgagcatc ccagcgcatc cagcaggcgc gtgtgatccc gcgccgccat caggggcagg
     3001 cggttctgga cgatctccaa ggccagctcg cgtcccgggg tcccacgctc gagcgcttgc
     3061 agctgcaagg ccaggcattc cgcgaggctg cgtgccgcga tgccagtcgg ctcgagcgtc
     3121 tggacgatgc gcagtgcagt ctcgatctcg ccgggctcga ctcctggtgc gaatctctgg
     3181 atctcctcga gcggctgacg cagaaagccg tcctcgtcga gcgcatcgac caccagattg
     3241 gccagggcat agtcgcgctc cccgagtccc agcagcaaca gctgctcgtg cagggagtca
     3301 tgcaaagtgg agggcgaggc ggtccattcg gtccagtccg gtgtgtcctc tccgccaccg
     3361 cccgagcccc cgccggagaa gtccagatcg tcatgggaac tctcggaagc ctcctcggcc
     3421 tgggtgcgcg tgtccggact ctccgccgct tcttccagaa agggattctg ggcgattgcc
     3481 tggtgcagtt cttgcacgca ctccagcgcg gagagctgta ggagcttgac cgattgctgc
     3541 agcctcggtg tgagggtgag ctgctgcttc aggcgcagtc ccattacggc gttcggcatt
     3601 ccaggagtcc tcgagcaaag cgatcgactg aaatcacagc aacagctatg ccagagcttc
     3661 gtggaaagcg gcagccactc ccagaggggt tcttctgccg cctcagcgcc cccgggagct
     3721 acccccgacg gagaacttgc aacggcgtgt aataaacgcc gcgccgcgcg ctgtcgctgc
     3781 gagatcaatg cgaacggtat gagatcaatt cacgacgacg gcctcggttc cgagcccagc
     3841 actgcctccg agagggcact cgcgctcgcg cgcgcgcaat acgcggctgt tgcgcagaag
     3901 aatgacgcac tcgaagtcgt gctggcgatt gctcgcttgg gttattgccg catcgatgct
     3961 ggcacgcgtg cggttgcagc gaactctcaa ttcaaggctg aattcggctt accgcccgat
     4021 gcggtgccga gctggccgca gattctcgac agagtcggca cggagcagcg ggccgcattc
     4081 agcgaagcca tcgagcgggc gctacgcgag ggcaacgatc ttgaatggcg tctgcagacg
     4141 ctctggcctg atggcacttc ccagtggatc tcactgcgcg gacgactgct gagcgatggg
     4201 gcagagggcg gcgcgctgtt tgtcatttcg cgcaatgtca ccgtggaaat cgcaacggca
     4261 gcgagcgaac aattgcgcca cacccagagt gtggaacacg agcagcggct gcggatggat
     4321 gccgaggccg ccaatcgcgg caaggatcag ttcctatcga tgttctcaca cgagctgcgc
     4381 tcacccctga atgcgatcct ggggtggaac cgcatcctct ctctgaagcg cgccgaggat
     4441 cctgaagtgg cctcgatcac agcgcgcatc gatcacagcg cgcgggcgca gctcaaaatg
     4501 gtcaacgatc tgctcgacct cggccgcatc gagaccggca agctgcggat cgaactgcgc
     4561 cccatgcggc tcgcccaggt cgctgccgcg gcgctcgatc tggcgcggcc cgtggccgcc
     4621 gcaaaatcga tcaccattac ctcggacttc gagcctggcg gcggagcgtt gcgcggcgat
     4681 ccggaccgcc tgcagcaggt ggtctccaac ctgctgtcca acgcattgaa gttcacgccc
     4741 tccgggggcc gcatcagtct cttgctacgc aaccgcaacg ggttcgccga gctgaccgtg
     4801 cgggacaccg gccagggcat cgaccccgat ctgctcccgc atgtcttcga caggtttcgc
     4861 cagggagaca gctcgagtac gcggcacgca ggcggattgg ggctcggact ggccctggtt
     4921 cgcgagatcg tcacgttgca cggcggctcc gtctcggcga tgagcgcggg ccccggtgcc
     4981 ggggcggcct tcacgatccg gttcccggcc cgacctgtct ggcgggcggc ggagaccgct
     5041 gcgccgggcg caaccgagtt gagccggtac ggcgacagtc tgcgcgggtt gtccatcctg
     5101 gtggtggatg acgaatccga tgcgcgcgcc gtcgtcgcgg agacgctcag actcgagggc
     5161 gcgcgcgtga cggtgacgga ttcggcgggt tcggcctatg ccaaagtgcg cgagcccgat
     5221 gcccatttcg acatcatcgt gacggacatt gcgatgcccg aggaggacgg ctactcgctc
     5281 gtgcgcaggt tgcgggctct acagactgga cgacagatgc tggcgatcgc cgtgacgggc
     5341 tatgcctcca agagcgatgt ggcggcagct ctggatgccg gattcgatct gcatgtcgcc
     5401 aagccagtcg acctcgatgc cttcgtgccg atgctgcggc gactcgccaa tgggggcagg
     5461 taagagctca gattcccgat tgccaggtgc tgggcatgcc tgctgcgccg atggcgtcct
     5521 cgatcaccag gcacacgtcc cgcgcttccg gctgcgcgcg caggcttcgc gcgatggaat
     5581 tcagaaacgt acacatcatg ggcacgttgg gatggcgtgc gtggagctgc tcctcgatgc
     5641 gatcgatgag ctctatcaca tcgccttgat cgcagctcgg gaaggtcatg ctggtcacag
     5701 cctgctgcac tgtgaccagc gcctggtgta ttgcgctctg ctgcgtgcca tccatggatg
     5761 cggaatttga actcttcgcc gtccgtctgc aactggttgc gcgcagggga aatcgatccc
     5821 gcgcggcatg gccggaaaac ggacaggaag cgggcaaaaa aagagcgggc tggtcgcacc
     5881 acagcccgcc aaacgggggg tcttcggtaa gcaccatcgc ccaccgggcc atggcgcgaa
     5941 tctcacaatg acgccctcgc gtccgcaaag ggcgtgcccg cgtcctaatg caaccgttgt
     6001 gccaggggag cccagagtgt gggaacgatc catgcttgag ctggtgcggg acatggaata
     6061 cgagggaagt gcgatgcgta ccgccgagac catctcgacg ctgaacggtt tgatcagggt
     6121 ctgccgaggg gccgaagcac gttgtcaatc ctgggtggcc acagcccagg ggactgctct
     6181 gcgcaggcga ctcgaagcgc gcgccggaga atggggaagg cagggcgatg agctacaggc
     6241 gttggttctg ctgctgggtg gcgagccggc gaggggcact tctttcgcgg cgctcctgca
     6301 tcggctatgg agcgcctccc gcgcagtgct gctcggccgc agcgacttgc cggtggcacg
     6361 cgcgttggat tggactcagc agcaggcact cgaatggtat gagcgcgcgc tcgagggcta
     6421 tcttccggag cgcatccgcc gaacggtcag cctgcacgcg cgacatattg ccgtgcgttg
     6481 cgagcggctc ggcgccggaa gcggccattt tgcctcgcct tgagaccccc tctgccgtgt
     6541 aaatcgtacc gaggctgaaa aaagttccgc ggtgccgcag ccttcctggc ggactgcagc
     6601 ctgccaaccg acttcagccg ttttctgcac atcacgaagt ccaccgagca tccttcggtg
     6661 ggcacatctc ctgctggatt gtgttccgcc gtgagtgggc ctgcagcgag cgcgcgcgcc
     6721 atgtcctcaa gcagtcggat tgtttccacg agctaccgcc acgcgactgt ggccttgggc
     6781 gagctgctgc ggatcacgcg cgacggcgag cagggattgc gcatgtgtgc aatccgcgcc
     6841 gaaagcggat ccctgcgcac tctgttcacg gcgatggccg atcgttaccg atcagcggcc
     6901 gccgaactgc acgagctgat cggccgcatc ggtggcgatc cgagcatgcg ctgtgtcatt
     6961 cccggcgccg cgcgtcgagg ttgggtaggc ctgcacgaga gtctggcgct gactgacgat
     7021 cgcgcgctca tagacgactg cgagcgcgcg gaggatcatg ccctcgaggt gtaccggaac
     7081 gcattggatg atcatctgcc ggagttcgtg cggcaggccg tgctgcaaca gttcgagggt
     7141 ttgatgcaag atcaccaccg gatccggttg ctgggccgcg agccgcatcc gaccggtgcg
     7201 gttgtcgtga gctacggtgg ccacacccag ccatagaatc gatttcgtcc tgctcaggcg
     7261 cccggccctc gcagcggtgg ttgccctggc tctcggcatg attctcggtc cactggggct
     7321 gcggattctg cagccggagc tggtggatga cggcgcaccc atcgagtcgc tgagcgagat
     7381 tgcgctgctc ctctgtctgt tctgcgctgg cctgcggctg cgcgtgccat tggaatggcg
     7441 ccattggcgg atgcccgggc ggcttgccac gctgacactg ggagcgactg tggccatagc
     7501 cgcggcagtg gcccacctga tgttcggcat gagcctgacc gagtctcttc ttctcggggc
     7561 gatcgtggcg ccgaccgact ccgtgctggg ctccgatgca gggccacacc ccgagggcga
     7621 tcacgagggc agcgcttttg tactcgcagc cgaaggcggc gccaacaacg ggctggccac
     7681 ggggctcgtg atgctggtcc tggcgatgat gggcctgagt gattcggact ctgacgcgct
     7741 gggtacgatt tcgctcgcgg cgttgtgggc tatcggtggc ggttttctcg ttggctggct
     7801 gacgggcgcg gcagccgcgc gctgcattat gttgttcgat ccggagcgga aaacggattt
     7861 tctcgaagag gggatggtct ttgcgacggc ctctctcgcc tatggggcag cgctgctgat
     7921 tcacacggac ggctttccgg ccgtttttgc cgcaggcgtt gccctgtgcc acggcggccg
     7981 gctgcgccga actctgcgca atcgacccct gatgccccgg gtactcagga tcgcggcccg
     8041 cgtggagcgg ttcgcctggc tggcgatcat cgttctactg gggtcgctgg tggcgagtgt
     8101 cgagttccgg gctcgcatgc tggtgtttgc gctgctgctg ctgctgctga tacggccgct
     8161 tgccgtgcgg ctggggctgg gaggcctggc ggtcccggag gctcagtggc gcagcatcgc
     8221 ctggttcacc ccccgaggca tcggctgcct gtactgcctg tcctttgcca tcaatcatgg
     8281 tttgagcgca ccgttctcgc gggagctggc cggaatcacg ctggtcgtaa tcgtcagttc
     8341 cctcattgcc agcgggatct cgggcttacc gctcaggcga gcctcaccag gtacggtcga
     8401 tctttagccc gcttccccgc ggccggccgc ggaccgtggt acgctccggc cagcccggcc
     8461 ccgccgccgg cacccgggtc ccgtccttgt ccggcgtccg gacgaccccg acaacgctgg
     8521 tgaccctcag ggggccgtcc tgggaccata atcgttacta tctggagaga tcagcccccg
     8581 cgcctgtctc gggagggcga caattgcagg cacggcgccg ggctctggac tgaatttcag
     8641 cttcgaggaa tcaatggcac acgctttgat agtcgacgac gacgccgatg tcgtggactg
     8701 gctgcaggaa gtcgcacgca tggaaggctt cacggtggcc cgagcccaca gcctgcggga
     8761 tgcgcgcatc gagctcggcc ggcagcggcc ggatgttctg ctgaccgatc tgcgcctccc
     8821 ggacggggaa ggcatcgagc tggtccgaga gctggaaaag ccagaagcca cggaggtcat
     8881 catcgtgacg gggcatgcga ccgtcgacag cgccgtggcc gctctgcggg cgggcgccag
     8941 cgactacctc gtcaagccgg cggatctcga tcgcgtgcag gccgtcctgc ggcacgcgcg
     9001 caagacctct gcactgcagc atgagattgg agagttgcgc gaagaactcc ggcggttggg
     9061 gcgcttcggc cgcattttgg gaagctcccc acgcatgcag gtgctttatg accaactcag
     9121 ccgggtggca ccgacttccg ccaccgtgat cctcatcggc gagagcggca cgggcaagga
     9181 gctggctgct gcgacggtgc atgagctctc gcgccgccgg gatgccgcct tcctgcctct
     9241 caactgtggc gcggtcgcgc cccagctcat cgagagcgag ctcttcggcc acgagcgtgg
     9301 cagcttcacg ggagccgacc gtcaacataa gggcttcttc gagcgcgcca acggcggcac
     9361 gatcttcctc gatgagatca ccgagatgcc gatggagctg caggtcaagc tgctgcgggt
     9421 gctcgagacc ggcacggtga cgcgggtggg ggggactcaa acgattccca gcgacgtgcg
     9481 cgtcatctgt gcgaccaatc gcgagcccga gaaggctgtc gtggacggca agctgcgcga
     9541 agatctctat caccggctca acgtgtttcc gatccgcctg ccgccgctgc gtgagcgcgg
     9601 gaccgacatc gagcagctcg cgcagttctt cctggacgag ctcaatcgca atgaaggaac
     9661 gaacaagaca ttctcgcgcg atgccctggt gcggctgtac cagcatgcct ggccgggaaa
     9721 tgtgcgcgag ctgaagaact acgtgcagcg cacattcatc atggccgatg aggtgatcga
     9781 gttcgagatc ggcaacgccg atagcgcgcc gaaggcagag gacaccacga cgatcaccat
     9841 ccgcgtcggg acgcccctgg aagaggttga gcggcgcgtg accatggcga ccctggcgta
     9901 ttgcggccac gtgaagcgca aggccgccga gattctcggc gtcagcctga agaccttgta
     9961 caaccgtctg gagacgtaca acggcaagga gtcgctgggt gataacaagg acgaggcggt
    10021 gaacgcgcag gactcgggtc actgacccca gggcatgaaa gccgctgcag cgcaacagag
    10081 aggcctcgta tgtcggacga ttcctacacg atcgcgaggc ttacgctgcc gtaccggctc
    10141 cctgactccc acatccgccc ccccgaaggg gcgagtacct tctctgcccc atggaaagta
    10201 cggttgcgaa gccctcacgc acaccaggca aacatagcgc cgtcatgcca gccacccgcg
    10261 ttgcagtcgt agacgatcat cagctcgtac gcctcggact caagcagatc atcgagagcc
    10321 agcccgattt cgcatgggca ggcgaggcca agaccggccg cgaggctctc gcgctgctgc
    10381 gatccgccgc gtgtgacgtg ttgctcctgg acctgtcgtt gccggacatg tccggcctgg
    10441 acgttctgcg acagatcaag gcgcatcatg agtcggtggc cactctggtg ctgagtgcct
    10501 atccagagaa tcaatacggg ctcaacgtcc tgagggccgg ggcgagcggt tttgtgagca
    10561 agactgccga tgacgccgag ttgtgccgag ccattcgcgc tgccgtgcgc ggcggccggt
    10621 atgtcagtcc agagctggcc gagatgttgg tgagcggcat gcagggcgct gcgggtgagc
    10681 cgcgccatag cgtgctctcg gagcgcgaat tccagatctt ctgcaagctc gccgagggga
    10741 agagcgtctc ggagatcgca gccaagctgt tcctcagcgt gaagaccgtc agcacttacc
    10801 gcacacggat cctggagaag atgagcatga agacgaatgc tgacatcacc tattacgcca
    10861 tcaagaacaa cctgctgcag tagctcatgg cgacggccca agtgcatcca cccgacgcca
    10921 gcgattcggc cgcggccgag cggcgcatcc tcctgatcga ggattcggca gtgctcacgc
    10981 gccggctgat cgatctgctc tcggagccgg gtcgcgtgga ggttgccgcc caggccagca
    11041 cgcagaccga agccctgcag cgcctgcagg aagccgcgtt cgatgtcctg gtggtggata
    11101 tcgagctcgc cgaaggcaac ggagtagccg tgatccggca tgcgcgccag gtgtatccgc
    11161 cacaggcaca gccactcatc gtggtgctga ccaactacgc ctcggacttc gtccgcgacc
    11221 attgctttgc ggcgggcgcc gattatttcc tcgacaaaat gcgcgacatc gccctgctca
    11281 aggcgatcgt ggtggacgga cactcgaagt gggcgccgcc gcccgcgagc cactgatctt
    11341 cggacgcacg cgccatggcg ccgggggtgg tgcctcgagc ggatcagctc tcgtgcggga
    11401 gcggaacccc gaagcgcaga gtcgtgcccg cgcctgcacg cgattcgacg ctcatcgagc
    11461 cgccgagcgc gtggatgcgc tggcgcatcc gcatcagccc gtgtgagctg gtgatctgcg
    11521 gtccggagct gtcgaatccc tgcccgtcgt cggtcagggt gaccatgcat tgcgccgcgt
    11581 tgccgccgag aaccagctcg acgcgccggc agcgtgcatg tcgcaagatg ttggaaaggc
    11641 cctcctgtac ggcgcggaac agtccgatgg ccatatcctt ggaaagggag tcacaggggt
    11701 gctcgacgag gacctcgcac tcgaggccgg cctgggcaca ggtggcctca acatgcgccc
    11761 ggattgcagt cgccagacca aaatgctcca gcagcgaggg tcgcagctgt tccacgatgc
    11821 ggcgcttgag gtccatggct tcgtcgagca cgctgccgag ctgtgccaac ctgtcctgca
    11881 cgggcttttc ctgccccggc gagagccgcg actgcagcca cgagagatcc atctttgcag
    11941 cggtcagcag gcccccgagc tcatcgtgga gcgtgcgtgc gagctgcgat ttctcctgct
    12001 cggcgagcga ctgcaggtga gttgagaacg ccgataactc gtgggtgcgc gcctccacga
    12061 gccgctcgag ttcagacacc tcgatgtcgc gctgctccac aatcatcgcc tccgcactcg
    12121 atttgccggg ctctcgggaa gcgttccggc acgccacctg ccggacctcc tgtgaagcgt
    12181 tccggcacac cacgcgctgc ggtttagcag atgcggctcg tgaccgccac ctcggctgcg
    12241 agcgcggtga cacctcgact gcagggcctc gcccaactag acggtcagga ccagcagcat
    12301 gccgacgatg atggccagca atgatgaaag gtcgcgaacg ctcatgatcc ggtgactctg
    12361 aatc
//
LOCUS       DASRHN010000040        12327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_342706, whole genome shotgun sequence.
ACCESSION   DASRHN010000040 DASRHN010000000
VERSION     DASRHN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342706"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFB37_01460"
     CDS             <1..218
                     /locus_tag="VFB37_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21139.1"
                     /translation="GRRAGLCELPRTGCAWYGRLSPPCWAARTYILKQLGSFQSNMRN
                     VAVMYGVALNLRLTDMEAVARYLESQP"
     gene            complement(357..1991)
                     /locus_tag="VFB37_01465"
     CDS             complement(357..1991)
                     /locus_tag="VFB37_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO21140.1"
                     /translation="MDKTVGVLAPTLADAEIAASVLREAGIGILAYGNFEELCSALGN
                     GIGAVVLVEEALSAEHLDRLASIRRSQPPWSSTPFIVLTRPHSAAARQPGNALATLPN
                     ALLIERPVRIENLLSAIRSSLDARSKQYAMRDLLEEARRSAAERSQLLKAAEDSRQQA
                     EAANRMKDEFLAVLSHELRTPMNAILGWAALLRRKALTAEQTERAVESIERNSRLQAQ
                     LIDDLLDVSRVISGSLRLDLQPIELEAVVAEAIATVTPASANKGVKIKLIGERAGNLV
                     RADSARLQQIIWNLLVNAIKFTPRDGTISVTIASEPGRVQVEIADTGEGIAPEFLPHV
                     FERFRQADSSTTRQRGGLGLGLAIVRQLVELHGGAVEARSAGCGLGATFIVRLPLIEV
                     AEETRHAGQRRTKHRRAGSLAASAQPLAGRVILLIDDDPESLQMASAAVSASGARVHT
                     AESAAEGVALATAIGPHAVVCDISMPGEDGYSFMRRLKSMGDVGANIPAIALTAHARD
                     EDKRAALDAGFALHLAKPVDPAELVRAVTQLVSASS"
     gene            complement(2016..3497)
                     /locus_tag="VFB37_01470"
     CDS             complement(2016..3497)
                     /locus_tag="VFB37_01470"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZO21141.1"
                     /translation="MANPQFVSTGIAGLDTVLGGGFLQGGFYLLQGDPGSGKTTVALQ
                     FLMEGARQGRRGLYITLTETRNDLQRAAASRDWSLEGVDIFDLTTTDVGALRESQYVI
                     FQPSEVELGETTKRVIAEVERIRPAQVVFDGLSELRLLASEPLKYRRQLLALKAYFDE
                     QQTTVLLLDDRSGAATSLNPESIVGGNIVMEQHLPGYGGARRRLHVTKVRGADFWDGY
                     HDYEIKPDGVLIHPRLVAAKHHADFPVAELPSGVENLDKILCGGLASGTTTLFLGPAG
                     VGKSTVAMQYVTSALQAKMKAAIFTFDEIISTLIGRSEKLVARGIVQYMQSGQLHLRQ
                     VDPAELSPGTFAHEVYRLIDEGVKVLVIDSLNGYLNAMPEERFLPTHLHELFAYLNQK
                     GIVTIVVVAQHGLTMTEGSELDVSYLADAVLLFRYFEVDAEIRQAISVFKKRTGGHER
                     SLRELQISSNGVKVGEPLVGFRGILTGVPEYLGVTKALGPPAR"
     gene            3903..5033
                     /locus_tag="VFB37_01475"
     CDS             3903..5033
                     /locus_tag="VFB37_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21142.1"
                     /translation="MRPVRRAGAPDTALVGASAGTDAEHSRVLVHGAHRLPSVDIDSY
                     SLELTDAEGFTGDQANKSAFVRILDDVRKLSRQAGEDPLGSKPSAEISRKKLAGLLEK
                     GDPHEAAVVLSAIEDFAQQLQSVIRVFLKQKSWQGTECIVVGGGLSGSRVGELAIARA
                     NIILKAEGVATDLQLLHECPDEAGLIGAAHLLPRWMLEGYEALLAADIGGTNIRAGLV
                     ELNLEKADDLSRARVLKFEDWCHREKDDVKRDEAVERLLKMLNELLDAGKKANLRLAP
                     IIGLGCPGVIREDGSIERGAHNLPGNWESSRFNLPQLVRERISHIGKHETVVIMHNDA
                     VIQGLSELPRLRDRAHWGVLTIGTGLGNARFSMRRSRKEQER"
     gene            5115..6266
                     /locus_tag="VFB37_01480"
     CDS             5115..6266
                     /locus_tag="VFB37_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZO21143.1"
                     /translation="MPPDFAALRREFPVLQRKIYLNSGSYCALANDVKAAFEAYMDDR
                     LAVGANWDVWITKNESVRALVATLLGASPDEIAVTASVSAGLNSLASALSFDGRRNKV
                     VVSDFEFPTNAQIWHAQEPRGAQVVHVPRAADGYIPPESFEPLIDERTQLVAITHVCF
                     RNGARLDIPAIVRLAHARGAQVLLDCYQSVGSLHIDVKALDVDFAVGGMLKYLLGTAG
                     IGFMYVREPLIASLVPTNSGWWAQADVHAMDITANRPAPSARRFEAGTPAVPNCYAAE
                     AGLKLLLQVGTMAIETRNLALTRLCMRRLQEIGWPSITPTEDARRGATVCVPSRNVGG
                     LCAELLKRDIVTSHRDDNLRASHHFYNDENDVDAFIAAMTALRREYAPA"
     gene            6280..6759
                     /locus_tag="VFB37_01485"
     CDS             6280..6759
                     /locus_tag="VFB37_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO21144.1"
                     /translation="MRKSHAHRPAPPAEAELFDDFLPYLVARLAHLLHLDLVEKLRHE
                     GINSTRWRILAVLAMEEGLTVNAIAERAMTQQSALSRALMTMEKERYVRRTSSPGDAR
                     YVQVFLTAKGRQLFHALNVVVRARESRLLEGLSAGERQSARRLMRRLLRNVAGGGVT"
     gene            6894..9164
                     /locus_tag="VFB37_01490"
     CDS             6894..9164
                     /locus_tag="VFB37_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21145.1"
                     /translation="MSRVATAACAVLTAVVLADGASAYAETDASFRLSAAFADLPAYF
                     PGYLGNGYISSLTAPRGTEPTRTYLVGLMDYAAGDMSRPASVPGWSEIDFSAGAAGSE
                     FGWLNRVPLNDRHFADYRQTLDLHEATLTTRYRYLDRGRETEVEVLSLVSQAAPHLAA
                     TRFMITPNYDGIVRLSFALTLWAPPAPRFPLAQLSGPQMEEAVTASGRSLQPQSPATA
                     DRAAVWYPGYIRILNSGGAGDSLSLWLDGQAEQGLPMALAASVSLPEGVHPESIRVHP
                     DRYKLALDVSVKVERGRTYTFTKYVALSRAGWGGKATEDLALARQARAEGFEHLLNEH
                     RTAWSALWQPDIQIETDAKAQQAVHSEMYYLLASSTADTAWAPGACALTPGYAGHIFW
                     DSDTWIFPALLLFHPERARSLVDFRERTLGAARQRAQQRGLAGAMFPWESDPEQGTEQ
                     TPHSAYVLGESEIHVNADIAIAQWQYFLATGDRDWLRTHGWLVIRDVARFWTSRASYD
                     AHTHRYDIQHVTSVSESNTGISNDTFTNVLAARTLSIATTVAGLVGERPDRRWQEIAR
                     NLYIPLAPQHQHHLAFDPAVVPQGNDFAGGPVSLLFLPSLDVEMSPELRRGDYEYGVR
                     PSSPALAGRVSMGIAPRSIAADTIGDETDAAAWFATNFSGGTLKPPFNVRTETADNNT
                     GYFLTGSGGFIQSLVYGFSGLRIRESGLVEAYAAVLPAQWNSLTLRNLSFRGRHLDIR
                     IARDHGGVVRLTRNLH"
     gene            9172..11955
                     /locus_tag="VFB37_01495"
     CDS             9172..11955
                     /locus_tag="VFB37_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZO21146.1"
                     /translation="MSPHAPRLRSVIRLLVTVLIGLLSACSRTSGPTVVAGNARFEFL
                     TPWLVRMEYARSGAFVDAPTAVVEKRDWPEVRVQTTRKDGWLVASTADVRIRYKLESG
                     EFSATNLEVTWKDRGGVQHAWRPGDKDPLNLGGLPYSLDNISQDNLPKGRPDTETPVN
                     DTIPGIDLLLAQAKPGLLSRAGYAFLDDSRTPVWNAQRTWIEPRAGTSLQDWYFFQYN
                     SDYQRVLREYAQLCGPVPMIPRYALGPMVTDLNFEYFPGSEQSRSPQFQHYDQQYLED
                     EVSRMRQNHIPFDTLVLDFAWHNYGWDGGYDWSPLIAHPRELSSWLHSQGVKLSLNDH
                     PGYINTEESILSFSDSHAPQVLKDLGRAEPLKPSFNLDISELWSFAYDPSDKGLSAHW
                     YADSARGVHWKHTRVGPTWQEQGFDGGRGFGWYRAVVRLPAQVPAKLYLYLGEVRKAY
                     RIFVNGREATPSSIHWPERLTYTDITPYVHPGRDNLIVLRVEPGEAGGGLVLGPVAIR
                     DVMPPKRIYFDLSNEQQAEVFMRDLHEPLMREGVDFWWVDGGSGAVDMPGLNKQLWTN
                     KVFYDFTQQHTGRRGFILARYGDWGSERYPAFFTGDTYSQWPVLAYEVAFTARGGNVL
                     MPYISHDIGGFHGTKIDFDLYARWIEFGTFSPLLRMHSAHENPREGNLRMPWVYGDQG
                     IALMKKYFTLRTQLIPYIYTYVWQAHQGSMPVLRPLYLRDPDNPEAYRHSHEYLFGDE
                     MLVAPVLDPSGMQVVYLPPGQWENFFTGQRHEGGSSFTAHYGVDETPVFVREGAVIPE
                     QPPSDYSDAHPLDKLILNVYGSGQGSFELYEDDGSTRDFDKGAYALTALRYNTSADGA
                     HHLTIGPATGSFKGQVPERSYELHIHAGEKPTAVSVDGRASQALSWDSSEAIAVVQLA
                     QRSIRDRIEVTWK"
     gene            11961..>12327
                     /locus_tag="VFB37_01500"
     CDS             11961..>12327
                     /locus_tag="VFB37_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21147.1"
                     /translation="MKQLCTWLAALGLTALPAFGAALSQPAQSSAGQPAVAQGPAYIQ
                     WLEERSMLHQAQELAKRYSGNSNQWQHPYGTPQPRAAVARASVWFTAYPASTIPDAPG
                     QSVLTTLADESLWRAFEEIG"
BASE COUNT         2248 a         3896 c         3896 g         2287 t
ORIGIN      
        1 tcggaaggcg tgccggcctg tgcgaactgc cacggaccgg atgcgcatgg tacggccgac
       61 tttccccgcc ttgctgggca gcacgcacat acatcctgaa gcaactcggc tcattccaga
      121 gcaatatgcg caatgtcgcc gtcatgtacg gcgtggcctt gaacctgcgc ctcaccgaca
      181 tggaggcggt tgcgagatac ctcgagtcgc agccctgagg acacccgcag tccccagtct
      241 caggtgcaga gatcgcgcag gagcctgaga ggctccgaga cctcagctca cataccgccg
      301 ccgctccggc tcccggaggg ccagctgcaa agtggcagct gcaaagctct cttcggtcag
      361 gaagaggcgc tgaccagctg cgtcacggcc ctgaccagct cggccggatc gacgggtttt
      421 gccagatgaa gcgcgaagcc tgcatccagc gcagcacgct tgtcctcgtc ccgggcgtgc
      481 gccgtcagag cgatggcggg gatatttgcc ccgacatccc ccatggattt tagccggcgc
      541 atgaaggagt atccatcttc tccgggcatc gagatgtcac agacgaccgc atgagggccg
      601 attgctgtag caagcgcgac gccttcggcg gccgattcgg ccgtatgcac acgcgccccg
      661 ctggcggata ccgccgccga agccatctgc agcgactcgg gatcgtcgtc gatgaggagg
      721 atgacccggc ctgcgagcgg ctgagcggat gccgccagcg aaccagcacg ccgatgcttg
      781 gtgcgacgct gtccagcatg tctggtttcc tcggctacct cgatgagagg caggcgcacg
      841 atgaaggttg cgccgagccc acacccggcg ctccgagcct ccaccgcgcc gccgtgcagc
      901 tcaacgagct ggcggacgat cgcgaggccc aggccaagac cgcctcgctg gcgtgtggtc
      961 gagctgtccg cctgcctgaa acgctcgaac acgtgtggca ggaactcagg tgcaatccct
     1021 tcgcccgtgt ccgcgatctc gacttggacg cgtccgggct cgctcgcgat ggtcacggaa
     1081 atggtgccgt cacgcggagt gaacttgatc gcgttgacga gcaggttcca gatgatctgc
     1141 tgaagtcgcg ctgaatcggc gcgcacgagg ttgcccgcgc gctcgccgat cagtttgatc
     1201 ttgacgccct tgttcgcact tgcgggcgtg acggtagcaa tcgcctccgc taccaccgcc
     1261 tcgagttcga tcggctgcag atccagtcgc agcgatccgg agatgacacg agacacatcg
     1321 agcagatcat cgatgagttg cgcctgcagg cgcgagtttc gctcgatgct ttcgaccgcg
     1381 cgctcggttt gctctgccgt gagggctttg cgtcgcagga gagcggccca gcccaaaatc
     1441 gcattcatgg gcgtgcgcag ctcatgactc aggaccgcca aaaattcgtc cttcatccga
     1501 ttggccgctt ccgcctgctg acgcgaatct tcggccgcct tgagaagctg cgatcgttcg
     1561 gctgcggagc ggcgcgcctc ctccaggagg tctcgcatcg cgtactgttt gcttcgggca
     1621 tcgagactgc tgcggattgc gctgagcaga ttctcgatgc ggacgggcct ttcgatgagc
     1681 aaagcattcg gcagcgtcgc cagagcgttg ccgggctgtc gcgccgcggc cgagtggggg
     1741 cgcgtgagaa cgatgaaggg tgtgctcgac cagggcggct gactcctccg gatgctcgcc
     1801 agacgatcga gatgttcggc gctgagagct tcctccacta gcacgaccgc tccgataccg
     1861 ttgcccagag cgctgcacag ctcctcgaag ttgccgtagg cgaggatgcc gatccccgct
     1921 tcccggagca ccgaagctgc aatctcggca tcggccagcg tcggcgccag gacgccgact
     1981 gtcttgtcca ttggcgcaag cgctgcgctc atcgtctatc tcgcgggcgg gcccaatgcc
     2041 ttggtgacac ccaagtactc gggtacaccg gtcagaattc cgcggaaacc caccaatggc
     2101 tcccccactt tgactccgtt cgaggagatc tgtagctcgc gcaagctcct ttcatgaccc
     2161 ccggttcttt tcttgaagac gctgatcgcc tgacggattt cggcgtccac ctcgaaatat
     2221 ctgaaaagca ggaccgcatc ggcgagatag ctcacatcga gctccgaacc ctccgtcatg
     2281 gtgagcccgt gctgtgcgac cacgacgatc gtcacgattc ccttctggtt caggtaggcg
     2341 aacagctcat gcaaatgagt gggcaggaac cgctcttccg gcatcgcatt caggtaacca
     2401 ttcaggctat cgatcacgag gaccttgacg ccctcgtcga tcagccgata aacctcatgg
     2461 gcgaaggttc cgggcgagag ctcagccgga tcgacctggc gcagatgcag ctgtccgctc
     2521 tgcatgtatt gaacgatacc ccgcgcgacg agtttctccg agcgtccgat caaagtggaa
     2581 atgatctcgt cgaatgtgaa gatggcggcc ttcattttgg cttgcaaagc cgaggtgacg
     2641 tactgcatgg ccacggttga ctttccgaca cccgcaggac cgagaaaaag tgtggtggtc
     2701 ccggatgcga ggcccccgca caggatcttg tccagatttt cgacgccgct aggaagttcg
     2761 gccacgggga aatctgcgtg atgcttggcc gcgaccaatc gtggatgaat cagaacaccg
     2821 tcgggcttga tctcatagtc gtgataacca tcccagaagt cggcgccgcg cactttcgtc
     2881 acatgcagac gtcgccgcgc tccgccgtag ccggggaggt gctgctccat gacgatgttg
     2941 ccacccacga tgctttcggg attgagactt gtagccgcgc cggaacggtc atccaggagc
     3001 aggacggtgg tttgctgctc gtcgaagtac gccttgaggg cgagcaactg gcgccggtat
     3061 ttgaggggct cggaagcgag caaacgcagc tccgacaggc catcaaagac cacttgtgcc
     3121 ggccggatac gctccacctc ggcgatgacg cgctttgtgg tctcacccag ctccacttcg
     3181 gagggctgaa atatcacgta ctgggactca cggagtgcgc ccacgtccgt cgtcgtgagg
     3241 tcgaatatgt ccacgccttc gagagaccag tcgcgcgagg ccgcagctcg ttgcaggtca
     3301 ttacgcgtct cggtcagcgt gatgtacaag ccgcgccgac cctgccgtgc gccctccatc
     3361 agaaactgaa gcgcgaccgt agtcttgccc gatccggggt cgccctgaag caggtaaaag
     3421 ccgccctgca ggaagccgcc gcccagaacc gtatccaggc cagcgatccc ggtcgaaaca
     3481 aattgagggt ttgccaatgc gttctccccg ctgagccgat tcttgttctc gtccctgagt
     3541 gttgcccggc gcggcgacgc ccgggagttc attgtacacg agcgcgccgg ggcgcccgcc
     3601 gaacgggctt ctgtcccgcg cccccgcatc aggctcgtgg cggcctcaag ggggcgcgtg
     3661 cttgagcggc tgcgcgtatc ccaatttggc gtcccgtcca tctgttgcct gcggctgcct
     3721 gcacaccaac tccgaggcag cctcgcgccc cccgtcccgc agagtccggg cgcggccctg
     3781 atcggaacga cgattgctac agggcgtgag gagaagccct gtaagtgcac caggtgcaag
     3841 aagatgccgc gcgaacagct ggagcaggaa atgccgagta acgactggga gagcgcattg
     3901 aaatgcgccc ggtgcgccga gcaggcgcgc ccgacactgc gctagtgggt gcatcagcgg
     3961 gcacggatgc cgaacactcg agggttctgg ttcatggcgc ccacaggctg cccagtgtcg
     4021 acatcgatag ctacagcctg gagctcacgg atgcggaggg ctttaccggc gatcaggcca
     4081 acaagagcgc cttcgtccgg atcctcgatg acgtgcgcaa actctcgcgc caagcggggg
     4141 aggatccgct cggcagcaaa cccagtgcgg agatcagccg caagaagctg gcggggctgc
     4201 tcgagaaagg cgacccgcac gaggccgccg tcgttctgag cgcgatcgaa gacttcgcgc
     4261 agcagttgca gagtgtcatc cgtgtcttcc tgaaacagaa gagctggcag ggaaccgagt
     4321 gcattgtcgt gggtggcggc ttgagcggca gccgcgtggg cgagctggcg atcgcccgcg
     4381 ccaacatcat cctcaaagcc gagggtgtgg cgaccgatct gcagctcctg catgaatgcc
     4441 cggatgaggc ggggttgatc ggggccgctc atctgctgcc gcggtggatg ctcgaaggct
     4501 atgaagccct gctggctgcc gacattggcg gcacgaatat ccgcgccggg ctcgtcgagc
     4561 tgaatctgga gaaggccgac gatctgtcca gggcgcgcgt gctgaagttc gaggactggt
     4621 gtcatcggga gaaggacgac gtcaagcggg acgaggctgt ggagcggctg ctgaagatgt
     4681 tgaatgagct cctcgatgcc gggaagaagg ccaatctacg gctggccccg atcatcggtc
     4741 tgggctgccc tggcgtcatc cgagaggacg gttcgatcga gcgcggcgcg cacaacctgc
     4801 ccggaaactg ggagagcagc cggttcaatc tgccacagct cgtacgcgag cggatctcgc
     4861 acatcggcaa gcatgagact gtcgtcatca tgcataacga tgcggtcatt caggggctga
     4921 gcgagctgcc ccgtctgcga gatcgagccc actggggcgt cctgacgatc ggtaccggac
     4981 tggggaatgc gagattctcg atgcgacgct cgcgaaaaga gcaggagcga tgagcatggc
     5041 gccgcatgca ttgagccaat atatgaaaat gcatatgatg gccgtccact gtttgagcag
     5101 gcagcgccac taccatgccc cccgatttcg cagctctgcg ccgggaattc ccggtcctgc
     5161 agcgcaagat ctatctgaat agcggctcct actgtgccct cgccaatgac gtaaaggccg
     5221 ccttcgaggc ctacatggat gaccggctcg cggtcggtgc caactgggat gtctggatca
     5281 ccaagaacga atccgttcgt gccctggtcg cgacgttgct gggtgcctcg ccggatgaga
     5341 tcgccgtcac cgcatcggta tcggccggcc tgaattctct ggcgagcgcc ctgagcttcg
     5401 acggccgccg caacaaggtg gtcgtcagcg acttcgaatt cccaaccaat gcgcagatct
     5461 ggcacgccca ggagccgcgt ggtgcgcagg tggtgcacgt tccacgcgcc gccgacgggt
     5521 atattccgcc cgagagcttc gagcctctca tcgatgagcg cacccagctc gttgccatca
     5581 cccacgtgtg tttccgaaac ggcgcgcgcc tggatattcc ggcgatcgtg cggctggcgc
     5641 atgccagggg tgcgcaagta ctgctggact gttaccagtc cgttggctca ctgcatatcg
     5701 atgtcaaggc gctggatgtc gactttgcgg tcggcggcat gctcaagtat ctattgggaa
     5761 ccgctgggat cggcttcatg tatgtcaggg agccactcat agcctccctc gtgcccacca
     5821 acagcggctg gtgggcgcag gcggatgtgc atgccatgga tatcacggcc aatcgcccgg
     5881 caccgagcgc gcgccggttc gaggccggca ccccagctgt gcccaattgt tatgcagccg
     5941 aagccggtct gaagctgctc ctgcaggtgg gcacgatggc aatcgagacc cgcaatctgg
     6001 cactgactcg gctgtgcatg cgccggctgc aggagatcgg ctggccgagc attaccccta
     6061 ccgaggacgc ccgtcgtggc gcgacagtat gcgtaccgtc ccgtaacgtt ggcggtttgt
     6121 gcgcggaact tctcaaacga gatatcgtca cctcccatcg cgatgacaat ctgcgggcga
     6181 gccatcattt ctataacgat gagaatgatg tcgacgcctt catcgccgcc atgacggcgc
     6241 tgcgtcgcga gtacgcgccg gcctgatctc gcgcgccgca tgcgcaaatc acacgcgcac
     6301 agaccggctc cgcccgccga ggccgagctg tttgacgact ttcttcctta tctggtcgcc
     6361 aggcttgcgc atctgctgca tctggacctg gtcgagaagc ttcgtcacga gggtatcaac
     6421 tcgacccgct ggcgcattct ggccgttctg gcaatggagg aggggctgac ggtcaacgcg
     6481 atcgctgagc gcgccatgac gcagcagtcg gcgctgagtc gcgcactgat gaccatggag
     6541 aaggagcgct atgtccggcg aacatccagt ccgggcgatg cccgctacgt tcaggttttt
     6601 ctcacggcca agggccggca gctgttccat gcgctgaatg tcgtggtcag agcgagggag
     6661 agccggctcc tcgaggggct gtccgcaggc gagagacagt ccgcacgccg attgatgcga
     6721 cggctcctgc gaaacgtggc gggaggaggg gtgacgtagc gggccgaggg gggatgagac
     6781 acgctgacac agctaggctg ccttcaccga ggtgtccggc gaagttgcac ccgtgagagc
     6841 gcgcaggtta ttttcttacg cttcgcccgc gatcgccgta aagttgaggt gagatgagtc
     6901 gggtagcgac tgccgcgtgt gccgtgctga ccgcggttgt gctcgccgat ggcgcgagcg
     6961 cgtacgccga gaccgacgcg agctttcgac tgagtgccgc gttcgcggat ctgcctgctt
     7021 acttcccggg atatctcggc aacgggtaca tctcgagcct gaccgccccg cgcggcaccg
     7081 agccgacccg cacgtacctc gtcgggctca tggactacgc cgccggggat atgtcgcgcc
     7141 cggcttccgt tccgggttgg tccgagattg acttcagcgc gggcgccgcc ggttcggaat
     7201 tcggttggtt gaatcgggtg cccctgaatg atcggcactt cgcggattac cggcagacgc
     7261 tcgatctaca cgaggcgacg ctcacgaccc gttaccgcta cctcgaccga ggccgcgaga
     7321 ccgaggtcga agtgctgtcg ctggtcagtc aggcggcgcc gcatctggct gcgactcgtt
     7381 tcatgatcac cccgaactat gatggcatcg tgcggttgtc gtttgctctc acgctgtggg
     7441 cgcctcccgc tccgcgattt ccgcttgcgc agctgagtgg tccgcagatg gaggaggccg
     7501 ttaccgcgag cggacgatca ctgcaaccgc agtcccccgc cacggccgat cgcgcggccg
     7561 tctggtatcc cggctacatc cggatactca acagcggggg cgctggggac tcgctgtccc
     7621 tgtggcttga cggccaggca gagcagggtc tgccgatggc tctggccgcc tccgtttccc
     7681 ttcccgaggg ggtgcaccct gagtcgatac gggtacaccc tgatcgctat aagctggcgc
     7741 tcgatgtgag cgtgaaggta gagcgaggcc gtacctacac gttcaccaag tacgtggcct
     7801 tgtcgcgagc agggtggggc ggcaaggcca ccgaggacct ggctctagcc cggcaggcgc
     7861 gtgcagaggg cttcgagcat ctgttgaacg agcatcgcac ggcctggagc gcgctgtggc
     7921 agccggacat ccagatcgag accgacgcca aggcccagca ggcggtgcac tcggaaatgt
     7981 attacctcct ggcgagttcg accgccgata ccgcctgggc ccccggggct tgtgcgctca
     8041 cccccggcta cgccggccac atcttctggg acagcgatac ctggattttc cccgcgctgc
     8101 tgctgttcca tcccgaacga gcccggtctc tggtggactt cagagagcgt accctcgggg
     8161 ccgcgcgcca gcgtgcacag caacgcggcc tggccggagc catgtttcct tgggagtccg
     8221 atccagagca gggtactgag cagaccccgc actccgcgta tgtattgggg gagagcgaga
     8281 ttcacgtcaa cgcggatatc gcgatagcgc agtggcagta cttcctggcg accggtgatc
     8341 gggattggct gcgcacgcat ggctggctgg tgatccgcga cgtggcccgg ttctggacca
     8401 gccgtgcgag ctacgatgcg cacacgcacc gatatgacat ccagcatgtc acctcggtct
     8461 ctgaatccaa caccggaatc tccaatgaca ccttcacgaa cgtgctggcc gccaggacgc
     8521 tgagcattgc gacgacggtc gcaggcctgg tgggcgagcg cccggatcgg cgctggcagg
     8581 agatcgcccg caacctctat attcctctcg caccgcagca ccagcatcac ctggccttcg
     8641 accctgccgt ggtgccgcaa ggcaacgact tcgcaggcgg tccggtctcc ctgttgttcc
     8701 tgccgtctct ggatgtcgag atgagtcccg agctgcgtcg gggcgactac gagtacggtg
     8761 tgcgcccgag ctcacccgcc ctggccggtc gcgtcagcat ggggatagca ccccgatcca
     8821 tagcagcgga cacgatcggc gatgagacgg atgcggcggc ctggtttgcc accaacttca
     8881 gcggtggcac actcaagccg cccttcaacg tgcgtacgga aaccgccgat aacaacacgg
     8941 gctactttct gaccggatcc ggaggattca tccagagtct ggtgtatggc ttttcagggt
     9001 tgcgaattcg cgagtcgggg ctcgtcgagg cttatgccgc ggtgctgcca gcgcagtgga
     9061 actccttgac attgcgaaat ctgtccttcc gtggccggca cctcgatatc cgcattgcac
     9121 gtgaccacgg cggcgtagtg cgcctgacgc gcaaccttca ttgaggtgag catgtcccct
     9181 catgcccccc ggcttcgcag cgtgattcga ttgctcgtca cggtcctcat cgggctgctc
     9241 tcagcgtgca gcaggaccag cggtccgacg gtcgtcgcag gaaacgcccg gttcgagttc
     9301 ctgacgcctt ggctcgtacg catggaatat gcccgctccg gcgctttcgt cgatgcgccg
     9361 accgcggtgg tcgagaaaag agattggccg gaagttcgcg tccagacaac ccggaaggat
     9421 ggctggctgg tcgcctcgac ggccgacgtg cgcattcgct acaagttgga gtcgggggaa
     9481 ttctccgcaa ccaacctcga ggtgacgtgg aaggatcgcg gtggggtcca gcacgcttgg
     9541 cgcccgggcg acaaagaccc gctgaatctc ggcggcctgc cttactcgct ggacaacatc
     9601 agccaggaca atcttcccaa ggggcgcccg gataccgaga cgccggtcaa tgacacgatc
     9661 ccgggcatcg acctgctgct ggcccaggcc aaaccgggtc tcctcagccg cgccggttac
     9721 gccttcctcg atgacagccg caccccggtg tggaatgcgc aacgcacctg gatcgagcca
     9781 cgcgccggga ccagcctgca ggactggtac ttctttcaat acaactccga ttaccagcgc
     9841 gtattgcgcg agtacgcgca gctgtgcggc ccggttccga tgattcctcg ctacgcgctc
     9901 ggtccgatgg tgaccgatct caacttcgag tacttccccg ggagcgagca gtcgcgcagc
     9961 ccgcagttcc agcactacga tcagcagtac ctggaggatg aggtctcgcg gatgcgccag
    10021 aatcacattc ccttcgatac gctggtgctc gatttcgcct ggcacaacta tggctgggac
    10081 ggcggctacg actggagccc gctcattgcg catccgcgcg agctctcgag ctggctgcat
    10141 agtcagggcg tcaagctgag cctcaacgat catccgggct atatcaacac tgaggagagc
    10201 atcctgtcct tcagtgacag tcatgccccg caggtgctga aggatctcgg ccgtgccgaa
    10261 cccctgaagc catccttcaa tctcgatatt tcggagctct ggtcgtttgc ctacgatccg
    10321 agcgataagg gactgtccgc ccactggtac gcggacagtg ctcgcggcgt gcactggaag
    10381 cacacccgtg tcggcccgac ttggcaggag caggggttcg atggcggccg ggggttcggc
    10441 tggtatcggg ccgtggttcg gttaccggcg caagttcccg ccaagctcta cctgtatctg
    10501 ggagaggtgc gcaaggccta tcggatcttc gtcaacggcc gggaggccac cccgagcagc
    10561 atccactggc ccgagaggct gacttacacc gacatcacac cctacgtcca cccaggtcgc
    10621 gacaacctca tcgtgctgcg ggtcgagccg ggcgaagcgg gcggcggtct ggtcctcgga
    10681 ccggtcgcga tccgggacgt catgccgccg aagcggatct acttcgacct ctccaacgaa
    10741 cagcaggcgg aagtcttcat gcgcgacctg catgagccgc tgatgcgcga aggcgtggac
    10801 ttctggtggg tcgacggcgg cagcggtgcc gtcgatatgc ccggactcaa caagcagctc
    10861 tggaccaaca aggtcttcta cgacttcacg caacagcaca ccggccgcag gggcttcatc
    10921 ctggcgcgct atggcgactg gggaagcgaa cggtatccgg cctttttcac cggggatacc
    10981 tactcacagt ggccggtgct tgcctacgag gtcgccttca cagcgcgcgg cggcaacgtg
    11041 ctgatgccgt acatcagtca tgatatcggc ggctttcacg gtaccaaaat cgatttcgac
    11101 ctctatgcgc gctggatcga gttcgggaca ttcagtccgc tgctgcgcat gcatagcgcc
    11161 cacgaaaacc cacgcgaggg caatctgcgc atgccctggg tctatgggga tcagggtatc
    11221 gcgctgatga agaagtactt cacgctgcgc acgcagctta ttccctacat ctatacctat
    11281 gtctggcagg cacaccaggg atcgatgcct gtgctcaggc cgctgtacct gcgcgatccc
    11341 gacaacccgg aggcctatcg tcactcgcac gagtacctct tcggggacga gatgctggtc
    11401 gcgccggtgc tggatccgag cggcatgcag gtggtctatc tgccccccgg ccagtgggaa
    11461 aacttcttca ccggccagcg ccacgagggg ggctcgagct ttaccgccca ctatggcgtc
    11521 gatgaaacgc ctgtgtttgt tcgagagggg gccgtgattc ccgaacagcc gccgagcgac
    11581 tattccgatg cgcatccgct cgacaagctg atcctgaacg tttacggctc cggccagggc
    11641 agcttcgagc tttatgaaga tgacggctca acgcgcgact tcgacaaagg ggcgtacgca
    11701 ttgaccgcct tgcgctacaa cacgagcgcg gatggcgcac atcacctgac gatcgggccc
    11761 gcgaccggct cgttcaaggg ccaggtgccg gagcgttcgt acgagctgca cattcacgct
    11821 ggcgagaagc ccaccgccgt atccgtcgat ggtcgagcga gccaggcgtt gagctgggat
    11881 tcctcggagg caattgcagt cgtgcagctg gcccagcgta gtatccgtga tcgaatcgaa
    11941 gtgacctgga agtgagaggc atgaaacagc tctgcacatg gcttgccgca ctgggcctga
    12001 ccgcactacc tgccttcgga gctgcgctgt cccaacctgc gcagagcagt gccggccagc
    12061 ctgccgtggc tcaaggccca gcgtatatcc agtggctgga agagcgctcg atgctgcatc
    12121 aggcgcagga gctggccaag cgctattcgg gcaactccaa tcagtggcag catccctatg
    12181 gaaccccgca gccgcgggcg gcagtggcac gcgcttcggt gtggttcacc gcttatcccg
    12241 cttcgaccat tcccgatgcg cctgggcaat cggttctgac taccctcgcc gacgagagcc
    12301 tctggcgcgc ctttgaggaa attggca
//
LOCUS       DASRHN010000041         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_14194, whole genome shotgun sequence.
ACCESSION   DASRHN010000041 DASRHN010000000
VERSION     DASRHN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14194"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VFB37_01505"
     CDS             <1..1396
                     /locus_tag="VFB37_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZO21148.1"
                     /translation="GWPQHTPELAKFYPTSALITGFDIIFFWVARMMMMGLKFTAEVP
                     FRDVYITGLIRDEQGDKMSKSKGNVIDPLDVVDGIGVEELVAKRTSGLMQPQLAASIE
                     RSTRKRFPQGIAAHGTDALRFTLAALASPSRDIRFDLGRVGGYRNFCNKLWNAARFVS
                     ISVGDGAAPGAEACEWSVADRWIRSRFGRMVQELEAAFRDYRFDFAATALYDFTWHAF
                     CDWYLELTKPVLLSDAASAALKRGTRRTLVEILEALQRALHPLMPFITEEIWQGVAPL
                     AGCPGESVMLEPYPLASDYPVDEEAEREIAWIQSFVLGVRQIRGEMDIKPSRHIPVLL
                     QGASERDRLYVTRHVTYLEKLAGLESVTVLQAGAAAPQAATALMGELSILVPMAGLID
                     VRAETERLDKQLVKTQQDLARARLKLANENFVRNAPAAVVETERERAAELERTVTSLT
                     AQLERLRNPDSRAS"
     gene            1404..2348
                     /locus_tag="VFB37_01510"
     CDS             1404..2348
                     /locus_tag="VFB37_01510"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZO21149.1"
                     /translation="MNAAAAIEPSTAALDRALSALEEVILGKPAQIRLCLACLLARGH
                     LLIEDVPGVGKTTLAHALAHVLGLAWRRVQFTSDLLPADIIGVSVFDRSSQQFHFRQG
                     PVFTQLLLADEVNRASPRTQSALLEAMEERQVSADLTTHRLPEPFFVVATQNPHEQLG
                     TYSLPESQLDRFLMRVTLGYPDSAHERELLRGGDRRDLLTQITPALTAEAVIRIQHDV
                     RSVYVSDAVLDYAQSLIARTRDRTDLKLGLSPRAGQGLLRAAQAWAYLERRRAVLPED
                     IQAILPPVIAHRLERREPDGGDGRTLATELIRAVPVPL"
     gene            2348..3373
                     /locus_tag="VFB37_01515"
     CDS             2348..3373
                     /locus_tag="VFB37_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZO21150.1"
                     /translation="MKHLLRERMARWIRRRQGLDILPVTLQRRRLYIVPSRAGVAFGL
                     LQILMLVAGLNYANSSALFLTFVLMGFTLVSMHQCHRNLLGLRVIAATALPTFARSPG
                     MLTLVLENPTAPTRYRIAAGLDRGPPVATDVPGSGRQTIALPVAAPRRGIVCVDRVLL
                     ITRHPFGLFLTWTWVHTPLQLLVYPYPQGALPMPADSGERPGQRPRTSTGQDEWFGLR
                     PFRDGDSPRQVDWKAYARESPLLVKEYSATASELRMFDFNALAGLPVEERLEQLARWV
                     VMAETAGERYGLLLPGVSIAADHGREHRHRCLASLATFALEEESSAAGFFSPTAVAAR
                     AGGFASG"
     gene            3392..5374
                     /locus_tag="VFB37_01520"
     CDS             3392..5374
                     /locus_tag="VFB37_01520"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HZO21151.1"
                     /translation="MSPRRGLEWAAGAFAAGVLLNADRVPTWVPVAALGFILWRLIAG
                     RAPVRLPGNIVRSVLAVLLVAAVLARFRTLNGLTAGTALLILMGGIKLLETRAQRDQY
                     IMIGASLFLLLAACLDRQTLLRAPLYALHAWLCCAALAVVASADSDHSFSNRAAVMLA
                     ARSLLYALPLGLALFVFFPRLAGAFWAIPRSSAAQTGLGDTMTPGSITALTASYAVAF
                     RARFEGLPPPPEERYWRGPVLHEFDGYTWRRGSGTAYRPQALEYLGKAYHYDIALEPS
                     SQRWWFSLDTAAGAPGLRAFFTYDYQLIAAEPVTEVTRYTLVSYTRTQSATELSSLAR
                     RRETLLPPDRNPRTLSLARKLRAEAGSDRAFIGAALELLRTGGFSYSLTPPALAQDSV
                     DDFLFNTHTGFCGHYASAFVALMRAGGLPARVVTGYLGGEWNPIGRYFIVRQSDAHSW
                     AEVWLQGRGWTRIDPTAVVEPERLRRGILDLLPDAVSAPARFVWSRPWLHGALERWDA
                     LNAWWNERIVRFSYDDQLRLLERLGFDAPGPRQLGWAFAAALMAWLLWIAWRLGRHPL
                     PGRPDRLARCYARLCRKLAGAGVAREPSQGPLAFAAEIDRRRPDLSGVVQPLLWQYAE
                     LRFGPPVPRPGTARLMQFERAVSRLRLAPWRETARR"
     gene            complement(5307..>5802)
                     /locus_tag="VFB37_01525"
     CDS             complement(5307..>5802)
                     /locus_tag="VFB37_01525"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21152.1"
                     /translation="VDYGLSLGLKQNSLITALLVVQFVGFPAALAFGWLGQKLGPRAG
                     ILIGIAVYSGIAGYAYFLHTEAEFMAMAIIIGLVQGGVQSLSRSLFGRLVPPGKAGEF
                     YGFYNLMGKAAAILGPTLTGVVALLTNDSRLAILSIVILFIIGALFLSKVPVSAATRP
                     ARTA"
BASE COUNT          907 a         1921 c         1915 g         1059 t
ORIGIN      
        1 tggctggccg cagcacacgc ccgagctcgc gaagttctac cctaccagcg cgctgatcac
       61 gggcttcgac atcattttct tctgggtcgc gcgcatgatg atgatgggcc tgaaattcac
      121 cgcggaggtg ccattccgcg acgtctatat caccggcctg atccgcgacg agcagggtga
      181 caagatgtcc aaatccaagg gcaacgtcat cgatccgctc gatgtggtgg acggcatcgg
      241 tgtggaggag ctggtggcca agcgcaccag cgggctgatg cagccgcagc tggccgcctc
      301 catcgaacga tcgactcgca agcgctttcc gcagggcatt gcggcccacg gcaccgatgc
      361 gctgcgcttc acgcttgcgg cgctcgcctc accgagccgc gacatccgct tcgatctggg
      421 ccgggtgggc ggctatcgta acttctgcaa caagctgtgg aatgcagcgc gcttcgtgtc
      481 aatctcggtg ggagacggcg ccgccccggg tgcagaagcc tgtgaatggt cggtcgccga
      541 tcgctggatc cgctcgcgat tcggccgtat ggtgcaagag ctcgaggcag ccttccgcga
      601 ctatcgcttc gactttgccg cgacggcgtt gtatgacttc acgtggcacg ctttctgcga
      661 ctggtacctc gagctgacca agcccgtgct gctctctgat gcggccagtg ccgctctaaa
      721 gcgcggaaca cgtcgcacgc tggtagagat tctcgaggcg ctgcagcggg cgttgcatcc
      781 actgatgccc ttcatcaccg aagagatctg gcagggagtt gcgccactgg cgggctgccc
      841 gggggagagc gtcatgctcg agccgtatcc cctggcaagc gactaccccg tcgatgagga
      901 agccgagcgc gagatcgcat ggatccagtc tttcgtgctc ggggtccgcc agatccgcgg
      961 tgagatggac atcaaaccct cgaggcacat tcccgtgctg ctccaggggg cctccgagcg
     1021 ggaccgcctg tacgtgacgc gccacgtgac ctacctcgag aaactggcgg gcctcgagag
     1081 tgtcacggta ctgcaagccg gtgcggctgc gcctcaagcg gccaccgcgc tgatgggcga
     1141 gctgtcgatc ctggtgccga tggcgggact gatcgatgtg cgtgccgaga cggaacgcct
     1201 ggacaagcag ctcgtgaaga cgcagcagga cctcgcccgg gcgagactca aactggcgaa
     1261 cgagaacttc gtgcgcaacg ctccggcggc ggtcgtggaa acggaacgcg aacgtgcggc
     1321 agagctggaa cgcactgtga cgagcctcac ggcgcaactc gaacggctac gcaacccgga
     1381 cagccgcgcg tcataatgag gccatgaacg ccgccgccgc gatcgagccc agcaccgcgg
     1441 cactggatcg cgcgctcagc gcgctcgaag aggtcatcct cggcaagccc gcgcagatcc
     1501 ggttgtgcct tgcgtgtcta ctggccaggg ggcatctgct catcgaagat gttcctggcg
     1561 tcggcaagac cactctcgcc cacgcgctgg cgcacgtgct cggtctcgcc tggcgccgcg
     1621 ttcagttcac cagcgacctg ctgccggccg acatcatcgg cgtgtcggtg ttcgaccgat
     1681 ccagccagca gtttcatttc cgccaggggc cggtcttcac gcagctgctg ctggccgacg
     1741 aggtcaatcg ggcgagcccg cgaacacaga gtgcgctgct ggaggcgatg gaggagcggc
     1801 aggtcagcgc agacctgacg actcatcggc tgccggagcc gttcttcgtg gtggcgacgc
     1861 agaatccgca tgagcagctc ggcacctact cattgccgga atcgcagctg gaccgctttc
     1921 tcatgcgggt gacgctcggg tatcccgact ccgcgcacga gcgagagctg cttcgcggcg
     1981 gtgaccgtcg tgatctgctc acgcagatca ctccggcgct caccgcggag gcggtgatcc
     2041 ggattcaaca tgatgttcgc tccgtgtacg tttccgatgc ggtcctggat tacgcccaat
     2101 ccctcattgc ccgcactcgt gaccgcaccg acctcaagct cggtctctcg ccgcgtgccg
     2161 gtcaggggct gctgcgtgcg gcacaggcgt gggcctacct cgaacggcgc cgcgcagtgc
     2221 ttcccgaaga cattcaggcc atcctgccgc ccgtcatcgc tcaccggctc gagcgacgcg
     2281 agcccgacgg cggagatggc cggactctcg ccacggaact gatcagggcc gtgcccgtcc
     2341 ccctctgatg aagcatctgc tgcgcgagcg gatggcacga tggatccggc gacgacaggg
     2401 gctggatatc ctgccggtga cgctccagcg ccgcaggctc tatatcgtgc cgagccgcgc
     2461 gggtgttgcg ttcgggctgc tgcagattct catgctcgtg gccggcctca actacgccaa
     2521 cagcagcgct ctgttcctga cgttcgtgct gatgggattc actctggtca gcatgcatca
     2581 atgccaccgc aacctgctcg gcctcagggt catcgcggcc acggcccttc ccactttcgc
     2641 gcgcagcccg ggtatgctga ccctggttct cgagaacccg accgctccga ctcgttaccg
     2701 gatcgcggcg ggtctggacc gcggaccacc ggtcgcgacc gatgtgcccg gttcaggccg
     2761 acaaaccatt gctttgcctg ttgcggcgcc gaggcgaggc atcgtgtgcg tggaccgggt
     2821 tctgctcatc acgagacacc cgttcggact ctttcttacc tggacgtggg tgcacacgcc
     2881 cctgcaattg ctcgtctatc cgtaccctca aggggctcta ccgatgccgg ccgacagcgg
     2941 cgagcggccc ggtcagcgtc cccgcacgag caccggacag gacgaatggt tcgggctgcg
     3001 tccatttcgc gacggagact ccccacgcca ggtggattgg aaagcctatg cacgtgagtc
     3061 gccgctgctg gtgaaggaat acagcgcgac cgcttccgag ctgcgaatgt ttgatttcaa
     3121 tgcgctcgcg ggactgccgg tcgaggagcg gttggagcag cttgcccgct gggtggtgat
     3181 ggccgagacg gccggggagc gctacgggct gctcctgccg ggggtgagca ttgcagccga
     3241 ccacggcagg gagcatcgac atcgatgtct ggcctctctc gcgacgtttg ccctggagga
     3301 ggagtcgagc gccgcgggat ttttcagccc gacagccgtt gccgcgcgcg ctggcggatt
     3361 cgcgagtggc tgacttgcag aggcggccgc tatgagtccc cgacgcggac tggagtgggc
     3421 cgccggcgcg ttcgcggccg gcgtgctgct caacgccgat cgcgttccga cctgggtgcc
     3481 ggtggccgct ctcggcttca ttttgtggcg gttgatcgcc gggcgggcac cggtgcgatt
     3541 accgggcaac atcgtgcgca gcgtgctcgc tgtgctgctc gtcgcggcgg tgctggcccg
     3601 ctttcgcact ctcaatggcc tcacggccgg caccgctctg ctcatactga tgggcggcat
     3661 caagctgctg gagacgcgcg cccaacgcga tcagtacatc atgatcggcg cttcgctctt
     3721 tctgctgctg gcggcgtgtc tggaccggca aacacttctc cgcgcaccgc tgtatgcctt
     3781 gcacgcgtgg ctgtgctgcg cagctctggc ggtggtcgcc agcgccgact cggaccacag
     3841 cttcagcaat cgggccgccg tgatgcttgc cgcccgcagc cttctgtatg cgctgccgct
     3901 cgggcttgcg ctttttgtgt tcttcccgcg cctggcgggg gctttctggg ccatcccccg
     3961 ctcgtcggct gcgcagaccg ggctcggcga caccatgacg cccggaagca tcaccgcgct
     4021 caccgcctcg tatgcggtcg ccttcagggc tcggttcgaa ggattgccac cgccccccga
     4081 ggagcgctac tggcgtgggc cggtcctgca cgagttcgac ggctacacct ggcggcgcgg
     4141 ctcgggtacg gcctatcggc cgcaagcgct ggaatatctg ggcaaggctt accactacga
     4201 catcgcgctc gagccgagct cacagcgctg gtggttctct ctggacaccg cggccggtgc
     4261 cccgggcttg cgagcgttct tcacctacga ctatcagctc attgcggcgg agccggtcac
     4321 ggaagtgacc cgctacacgc tcgtttccta caccagaacg cagtcggcta cggagctctc
     4381 ttctctggcg cggcgtcgcg agaccctgtt gccgccggat cgcaatccgc gcacgctcag
     4441 ccttgcgcga aaattgcgcg ctgaggcggg ctcggaccgc gccttcatcg gcgcggcact
     4501 cgaactgctc agaacgggcg gcttcagcta ttcgctgact ccacccgcgc tggcgcaaga
     4561 ctctgtcgat gactttcttt tcaacaccca cacgggcttc tgcgggcact acgcgtccgc
     4621 gttcgttgcc ctgatgagag ccggaggttt gccggcacgc gtcgtgacgg gatatctggg
     4681 cggtgagtgg aacccgatcg gacgctactt catcgtgcgc cagtcggatg cccactcctg
     4741 ggccgaagtc tggctgcagg ggcgtggctg gacccggatc gatcccactg cggtggtcga
     4801 gcccgagcgc ctgcggcgcg gcatcctcga tctgctgccg gatgcggtct ctgccccggc
     4861 gcgctttgtc tggtctcgac cctggctgca cggggctctc gagcgctggg atgcactcaa
     4921 cgcgtggtgg aacgagcgca tcgtccgctt cagttacgac gatcagttgc ggttgctcga
     4981 gcgtctcgga ttcgatgcgc cggggccccg tcaactgggg tgggcgttcg ccgccgctct
     5041 catggcgtgg ctgctgtgga tcgcctggcg cctcgggcgc catccgctgc cggggcgacc
     5101 tgacaggctc gcgcgctgct acgcgcggct gtgccgcaag ctcgcaggtg cgggcgtggc
     5161 tcgtgagccc agccaggggc ccctcgcctt cgccgccgag atcgaccggc gccgcccgga
     5221 cctcagcggc gtcgtgcagc cgctcctgtg gcagtacgcg gagctgcgct tcgggccgcc
     5281 agtcccacgg ccgggtaccg cccgtctcat gcagttcgag cgggccgtgt cgcggctgag
     5341 actggcacct tggagagaaa cagcgcgccg atgatgaaca ggatcacgat ggagagaatg
     5401 gccagacgcg agtcattggt gagcagcgcg acgacaccgg tgagtgtcgg gccgagaatg
     5461 gccgccgctt tgcccatgag gttatagaag ccatagaact cgcctgcttt gccgggcggc
     5521 acgagtcttc cgaacagcga gcgggacagc gactgcaccc cgccctgcac cagtccgatg
     5581 atgatcgcca tggccatgaa ctcggcctcc gtgtgcagga agtaggcgta acctgcgatc
     5641 ccggagtaca cggcgatgcc gatgagaatt cctgctcgcg gcccgagctt ctgtccgagc
     5701 catccgaagg cgagggcggc ggggaagccg acgaactgca ccaccagcag tgcggtgatg
     5761 agactgttct gtttgagtcc gagcgagagg ccgtagtcca cc
//
LOCUS       DASRHN010000042        25447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_838917, whole genome shotgun sequence.
ACCESSION   DASRHN010000042 DASRHN010000000
VERSION     DASRHN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 25447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25447
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838917"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1106
                     /locus_tag="VFB37_01530"
     CDS             93..1106
                     /locus_tag="VFB37_01530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO21153.1"
                     /translation="MRTCDVLVVGGGPAGSSAARQLKLGGASVLVLDKERFPRLKLCA
                     GWITPEVVRDLGMDVASYPHRFLTFERLHVHLKGLHFRLSSVQHSIRRFEFDAWLLER
                     SGAEVVQHTVRNIRAQAGEYIVDELFRCRYLIGAGGTRCPVYRTLFRELNPRATELQT
                     VTLEHEIEYPWKDPDCHLWFFERGLPGYSWYVPKQNGWLNVGVGGMAERIKSSRGDIR
                     DHWSHLQQTLERSLAPGANYEPTGYSYFLRGRVEVVRRDNAFITGDAAGLATRDMAEG
                     IGPAIRSGQRAARSILEGAAYSLQDVTGASLGGGWVSRALDWAMTRGHAPLRTPAVHS
                     PAK"
     gene            complement(1027..2997)
                     /locus_tag="VFB37_01535"
     CDS             complement(1027..2997)
                     /locus_tag="VFB37_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HZO21154.1"
                     /translation="MSARFPDTPMGANLVDGGATFRTWAPHAKAVHVVGDFNDFTPAA
                     ENALTADSEGHWRGFIPGVLEGQRYKFWITGEDGPGYKRDPYAREIRGAQWYCIVRRP
                     TFPWHATDFVAPALPDIVLYQLHVGAFYTPRCPPYPGTFLDVLLKVPYLVELGINVVQ
                     LLPIQEFPGCFSLGYNGTDYFAPEPAFAVGDERLAPYRSQVNQLLTAKGLQPFALTDL
                     QGEVNQLKALIDVCHIHGLAVIFDLVFNHAGGGFGAESLWFFDRQRGADEDPPRYWSS
                     LFFSDQTWAGGNVFDFKSEGVRRFLIDNARFFLEEYRVDGFRFDEVSVIERCGYGRGW
                     DFCQELTARLRSLRPGLLLHAEYWPVNPAVVKDTRDGGAGFDTTLTDGLRIALRNVLA
                     TAARADDEPLPLSVLADHLAPSYLRDLWRGVQDIENHDLVLRPKDPMDHNRMQRIARA
                     ADPANPRSWWARSRSRVATGLVLTAPGIPMLFMGQEFLEDKQWSDDVLDLPELLLYWP
                     GLEAPDPAMRDFLRFTRELITLRHELHALRSEGFKVVHVHDRNRVLAFHRWVPAQGRD
                     VLVVASLCNSHQYGYRIGFPLGGSWREAFNSDAYDNWINPQVAGNWGRVVTVPVPLHG
                     CAWSAPVTLPANALLVFEAEHGPASSPSRGPG"
     gene            complement(2994..3680)
                     /locus_tag="VFB37_01540"
     CDS             complement(2994..3680)
                     /locus_tag="VFB37_01540"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZO21155.1"
                     /translation="MRHDGEKSAVMKGKDETREYSSPACLLHEFETTPPSPSERWEDV
                     KLWRKARREELLARRAQRSAEERLSLSAPILEYLFENIDASKCPVLGLYWPIRGEIDL
                     RALARRHVESGGIVGLPVVVQKAAPVEFWRWHPGVAMRRGLWDIPVPPERAPVTPQLL
                     VIPLVGFDGARFRLGYGGGFYDRTLAAAAPRPKTIGIGFADAQLPTIYPQPHDVPMEL
                     VVTDRFTLPA"
     gene            complement(3870..4571)
                     /locus_tag="VFB37_01545"
     CDS             complement(3870..4571)
                     /locus_tag="VFB37_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21156.1"
                     /translation="MHQQNARALDPRREAHDAEVTLGMTAEHHTAHHRTGNPRLDLIL
                     GLSAMFVSVVSLGVAIQHGHAMDRLVAANSWPFLMYTTANRDSQGHERISLTLMNQGV
                     GPARIETFEAWWQNQPVTSALDLLTRCCGLDSDTGDRAAPMPHGTVSLGAVAPLVLRP
                     GDSQTFLTLDRSEANAEIWQRLNAARIQIKMRACYCSVFDECWVSDLQHTNAQRAKSC
                     PAVKVPFEVPANWSQ"
     gene            4702..4893
                     /locus_tag="VFB37_01550"
     CDS             4702..4893
                     /locus_tag="VFB37_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21157.1"
                     /translation="MRTLLLAALFIGVVGLVSAYTLRIRAEAIATSQHLVYAAMGAPR
                     FPGGCPPVVRVTTLDLCAF"
     gene            complement(5030..5656)
                     /locus_tag="VFB37_01555"
     CDS             complement(5030..5656)
                     /locus_tag="VFB37_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21158.1"
                     /translation="MSFLRVAPMLAALFLCGCAETLAYRPEDVPDTPIPYPDRITGKI
                     LIYTTRTDDDRSITAGSTSLTGAGLRLTVPVGLMVREIALKVFSKAASAGADESDDFT
                     TASRYSIALRPQIENFEYGFPHPKGVEVKPEVRMTLRISLLDATGRVLLEKDYDSGVV
                     SGSRHLISGKLVERTDKLTHEVMFDLMQRAAADVHLYEQTQARAAAAN"
     gene            complement(6351..8171)
                     /locus_tag="VFB37_01560"
     CDS             complement(6351..8171)
                     /locus_tag="VFB37_01560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZO21159.1"
                     /translation="MKSNFSGRRQWMSSSLGLGLLLFWLACWAEAAPGAADTVYTGGV
                     IYTMDARDTVAQALAVAGGSIVYAGSDAGARRYVGHNTRLVPLKGRAVMPGLIDGHMH
                     PVEGGLTLLKCSLNYESLSIAEFQAHIQACLDQHRSNSAEAWFEVVNWFRYGLRPQGA
                     TVSRATLDALKTDRPIVVRDTFGHSSLVNSRALALAKITAKTSDPANGRIGRYASGEP
                     DGFLEDAAQNLVLERVPKPTPAENLAAVRAALNAMRRQGVTAFLDAVAETPDIEAFAA
                     IEKAGGLTARAHFAPRIRPEQTPDAPSARRAVTRVVALARQYDEGPLRPRPSISVHNA
                     KLFMDGVITAPANTGALLQPYYENHGSAAHPKFEPAAKREPDVYFPAPILREILIALG
                     EAGIDPHLHTDGDGAVRAALDGIAQMRQALPGRDVRPALAHCELVDPADYPRFAELNA
                     IPVLSFQWEKPAFDSIEGARDTLGPRRHALIEPAGVLAARGARIAYGSDWPVDPLDEW
                     FALQVGVTRMDRPGGPPQHAGRLGTDSGLSQKEVLRAITANAAYELHEDHFVGTLEAG
                     KLADFIVIDRDPAAVPPTEIAATRVLRTIVGGQEVFRSEP"
     gene            8318..8650
                     /locus_tag="VFB37_01565"
     CDS             8318..8650
                     /locus_tag="VFB37_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZO21160.1"
                     /translation="MDHSHGFKRRQNFILPLAVALAASWTVGYAADPTDDATVKSQVE
                     AALNGDSNLFAKHIDVSVKHGVVRLSGFVQKQTELERAKKDAAGVPGVMSVKNELTLK
                     HNETGASH"
     gene            8940..9116
                     /locus_tag="VFB37_01570"
     CDS             8940..9116
                     /locus_tag="VFB37_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21161.1"
                     /translation="MSQKPTPLTDAVVCRDEKGNIVITGKVNGREVVHTVTPRPKSVQ
                     RQLSLYKKLFADLG"
     gene            complement(9120..9617)
                     /locus_tag="VFB37_01575"
     CDS             complement(9120..9617)
                     /locus_tag="VFB37_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21162.1"
                     /translation="MALHLSDEALQDSAELSAAAPTKAQIRQAVRRQIAAELYDESAG
                     DGTGTAIYTLSDPRDLRLIRYVGQTRAPKGRFRQHLNSARLWLPDALPWWVKSPKLRP
                     LYQWIRDLYRDDGRLPTMIVREWQVSASQVRAAERARICECLAQGLPLLNYEAEILIR
                     QLRLL"
     gene            complement(9739..11154)
                     /locus_tag="VFB37_01580"
     CDS             complement(9739..11154)
                     /locus_tag="VFB37_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21163.1"
                     /translation="MRLLAISRLGPLGLLLFALLLSNVSAAATRVWVANNGVDSPSCG
                     AASNPCRSISQGIENAQSGDTIYVGPGHYGNVSGSADYSGPGDERPQTFVNDSEGCLV
                     CITKSLHIYSVSGASVTVIAGSPPPQGSGISMANVAILQDGVAFGSAGHGFTLTGGSS
                     VGLEIDYQTMEDVSSVGPSRNISVSGNVDIGDGSGFVFSGRTETTQECPTSSPVCAPT
                     AQLLFSDNESYGNGGFSYSATANMDLGPGQVLFENNLAMGSGGGFQIQTGFQCKTCEV
                     AIRASNIQWRNNVATNSGIGFYANMPGEISGNTAAYNSQAGFQVTPGPPSLFQHNSAI
                     GNSGPGVIIQWSPDAFTEFGGSFQTFSQNNFFGNDRNRPTLALHTGDGGGGHGPYNPG
                     PSAHCGVLNVGALADIIGGPAGTPPPRAPLAANYDYWGSAAGPKATGAGDAIGGACDQ
                     NNAVTTARFPAATAYPVTTAP"
     gene            complement(11440..12828)
                     /locus_tag="VFB37_01585"
     CDS             complement(11440..12828)
                     /locus_tag="VFB37_01585"
                     /inference="COORDINATES: protein motif:HMM:NF005460.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZO21164.1"
                     /translation="MSSDSPPTLASLAQALAAGRTSSRALVAECLERIADKQGEGART
                     FLKVHAAQALATAEFHDQMRRHGAASSPYAGIPISVKDLFDIAGDTTTSASAVLRDAP
                     AAQLDCPAVARVKAAGFVVIGRTNMTEFAFSGLGLNPHYGTPLNPWDRATGRIPGGSS
                     SGAAVSVTDRMAFGALGTDTGGSCRIPAALCGIVGYKPTAHRVPKSGVFPLSSTLDSV
                     GPLARSVACCRALDGVLSGDIPSAALQELASGGVDRHSAAAVRGLRLGVLTSYVMDGL
                     APQVAADYQRALRALSTAGASLTDVDVPELGELPGLNRKGGIAAAEAYAVHRTRLNSD
                     ASRYDPRVANRIMRAREQDAADYIELLHARVAFIERVSRRLIDFDAVVMPTVPVSAPA
                     LSELSADDVYVRTNALILRNSSVVNFLDGCAISLPCHEPGTAPVGLTLFGLRDADERL
                     WPVAAAVEQCLL"
     gene            complement(12834..13817)
                     /gene="speB"
                     /locus_tag="VFB37_01590"
     CDS             complement(12834..13817)
                     /gene="speB"
                     /locus_tag="VFB37_01590"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZO21165.1"
                     /translation="MDKPVQSPGSAISFNQPLGGNSMPRFAGPATMMRLPTAASAVGL
                     DACFIGVPLDIGTSNRSGARFAPRQIRAESCLIRPYNMGTGAAPFDSMQVADVGDVAI
                     NTFDLAESIEIIERAFEEVLRTDCVPLTLGGDHTIALPILRAMRRKHGPVGLIHVDAH
                     SDTNDTMFGSKLAHGTPFRRAIEEDLIDCRRAVQIGLRGTGYSSSDFDWTRERGVRVV
                     AAEDCWLRSLAPLMNEIRASFGRGPVYLSFDIDGIDPSFAPGTGTPEIAGLTVPQALE
                     IIRGCRSLQIVGGDLVEVAPPYDSTGNTSLLAANLLYEMLCVLPKTVYRKR"
     gene            13959..16073
                     /locus_tag="VFB37_01595"
     CDS             13959..16073
                     /locus_tag="VFB37_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO21166.1"
                     /translation="MGRQPRYDVLFEPVRIGPVTAPNRFYAVPHASGMTNTMPRMRAG
                     FRAMKAEGGWGVVCTGYVSVHPSSDDSPLPYATLWDDNDIRSHALMTEAVHRHGALAG
                     IELWHGGGSVSNKTSRFPPLSPSGLSWMATHVGFMGNARSKAMDAADIRNLLHWQREA
                     ARKARSAGFDIVYVYAGMGYLPYEFLLPEWNLRTDAYGGAIANRVRLVRELLEVTRDA
                     VGDTCGVALRISLEELRSRRSEHAPSEAHEVVELLSDSTDLWDVKMDSSPTDCGPSRF
                     TPEGSHEPVIDFVKRITGKPVVGVGRFTSPDTMVSQIRRGVLDLIGAARPSIADPFLP
                     RKIDEGREQDIRECIGCNICISSWHDSVPVRCTQNPTIGEEWRRGWHPEHYGAPGRGS
                     TVLIVGGGPAGLECALTLARRGYSVSLAEAADHFGGRLRFETSLPGLATWSRVLDWRL
                     SQLRRLSNVTLYAGNELSVEDILGLEHQRVVVATGARWTRMLYSTLELAVGDLEAANV
                     FTPDDVAAGKLIEGPAVVFDFDNYYMGSALAEYLAQSVDRVSYVTPAGHASAWTIMTN
                     EQPQVHRALANAGVKLHTLCRVTRLERDEVVIANLFTGEERQIPCRSLVIVGARAARD
                     DLHRALVARQEELSAAAILSVDRIGDALAPGAIVHAIHGGHRYGRELDAELGSDLYRR
                     DAPIVDEAPLLYDDGSAEQAGK"
     gene            16070..17239
                     /locus_tag="VFB37_01600"
     CDS             16070..17239
                     /locus_tag="VFB37_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZO21167.1"
                     /translation="MKTSEVAIIPGLEQTLPSSWYYSEEVYRLERERIFCREWLCAAR
                     AEELPNPGDYRVQDIVGESILLVRNREGTLRAFYNVCRHRGSRLCRADGRGPTGMAVQ
                     GGLIAGRSIMCPYHQWTYDLDGHLIAAPHLGSVTGFDKSEISLYPVGVECWGGFVFVH
                     LTPAEAQPLAAQLGEIPARIARYPLAELRIGHSIAYEVKANWKSICENYNECYHCGGV
                     HPELCSVVPAFREAGGGGLDWMRGIPHRQGAYTFTRSGTTARRPFPSLNQDERERHKG
                     EVIYPNLFVSLACDHAAVFILQPRGPERTDILCHFLFEPHEIAQPGFDPADAVEFWDV
                     VNRQDWEICESVQRGMRSRVHQFGYYAPMEDFNLDIRRYVHERLDPAPDRPCDDA"
     gene            17236..18582
                     /locus_tag="VFB37_01605"
     CDS             17236..18582
                     /locus_tag="VFB37_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21168.1"
                     /translation="MSSNGLIAQVDSLTAPTPLWALTHRVRWRIYSFLFVFGCIAYFQ
                     KLGLTVAADRMMPDLHITQAQLAWLEWALVLGYTAAQFPGGILGRRLGARRAFVLISC
                     FALLGTVLTPLAPELLHGMSLIGVLLALQLVVGLAQGPIFPVSSGVMEAWFPPEKWPL
                     VQGLQSAGLQLAAALTAPIVAYLMTFLGWQQALFWPALPAVLVIAAWGWYARNTPTEH
                     RAVTAQELAELGPIPPAQPQGPMSWERLRRLLSNRSILLLTCSYVCMNYSFYLIANWC
                     FLYLVQERHFNVLEGGWLATLPPLAAAVGAGLGGKLVMIACARLGNRWGFRAVPLLAL
                     PLTGVLLLATVHLGNPYAAVIALALAYAAVELTEGPYWAGTMFIARSETMPATGVLNT
                     GGNLGGLIGIPIVGYLSGSGHWTFAFGLGAIFSVLGALAWLGIDAEQSFDRPAGRT"
     gene            18657..21200
                     /locus_tag="VFB37_01610"
     CDS             18657..21200
                     /locus_tag="VFB37_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO21169.1"
                     /translation="MPDFRKRNPLIAAAIATALAGTRLAHAADADAAPTASTPAPVLQ
                     EVVVTANRREEDVLTVPYNISAISAAQIQDAGVKDLEGLTRMVPGLVAADLGPRVNST
                     NSTLIIRGLNASDEGSNYFGPNLAVPLVSTYLDDVPLFVNFNLTDIERVEVLRGPQGT
                     LYGSGAVGGTVKLLRHKPDLGVFSVDFTADTSGTEHSQGPSYSFSGIVNVPVSDTVAL
                     RASAGMTKITGFIDASHVARFQPNGQPLLADPSAPLTSDYQYTTKKDVDSATSEYGRL
                     SMLWKPSEVLSVLFDYTHQEDHSGGFSAETVNFNYATGAPIPIQPYTTTTPLPEQSLN
                     RLLDIGALTVTADFGFATLTSSTSYYDNRYNDVIDVSSTQQYFSLANPYYYGGYPRFA
                     AFNFDYSKDESLVEELRLVSKTGGAWDYIVGAFFRTQKEALSDPEIWPGLASWSHLPG
                     SANAYNYYAGTTFNTFADVIASRDGSDPESLKPTDLFYNNIRHTRFKDRALYGELTRH
                     LTDRWQVTGGAREFWQAYDQVETQIFPFAGAFYSDTGTDPLGDTTSEQAKSYRNHIFK
                     LNTSYEISPAARVYATWSEGFRHGGANSFKICPSGAPPLGGCEPAESAALIPYVPDTA
                     KNYEVGIKGEIGGRVRYSTSLYRVDWHNIQLDAFAPVTGIQIVANGKDARSQGVELEV
                     QALVTTGLSATIGGSLTDAHLTDSFVTANFIGQKGNALPLVPRTQLTAALDYVFLLPQ
                     ERDVSFHMDAAYRSAVDTSVNDLQVDANGNTFVDIYATNFRRLSGFTTLNAGIGFQAS
                     KQVKLRLYCNNITDQKGVTTWSVAHQPEQSFEYVMRPRTAGIELDYSFR"
     gene            21304..22866
                     /locus_tag="VFB37_01615"
     CDS             21304..22866
                     /locus_tag="VFB37_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO21170.1"
                     /translation="MTRLLQAGQLKAAEEQCRTLTSDFPLFLPGWHSASFIELCLGRL
                     GPALESIGRALASSPADPRYRLQHARCLSALGKLPEALASAAIAESGALADAALLDAI
                     GSFYSATGEQHRALQAYNRAIALDPQQAVFWFNRAAVRRFLGDLDGAEADYDRCIALR
                     PEDHEAYLNRSELRRQRPERNHLEVLEGLLGAGVRQWRGEVQIRYALAKEYEDVGDYA
                     SSWRHLQSGARLRRQHLQYDVLYDVRTVDWIMEAFASAPKEPVGGCPSAAPIFIVGLP
                     RSGTTLVERILGSHSEVHAAGELNHFAAALVAGVQAGRGDRALARRELIGATRQLDFT
                     ALGVAYLQRAHASTLGRPRFTDKMPLNYLYCGLIHRALPNARIVHVTRHPMGSCYAMF
                     KTLFKEGYPFSYDLGDLAKYYAGYRRLMQHWNATLPGVIHEISYERLIAAQETETRRL
                     LEACGLGWQEACLEFHRNPTATTTASAAQVRQPLYDSSVMQWRHYEEQLVGLRAELAS
                     LGVTWQDAGPLQ"
     gene            22905..24155
                     /locus_tag="VFB37_01620"
     CDS             22905..24155
                     /locus_tag="VFB37_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZO21171.1"
                     /translation="MPQATAATIFDASTCERCRADFPSLARRFGPHTVAFLDGPGGTQ
                     VPNAVIEAIADCYRKRNVNTHGNFPVSAELDERHYQMRAIVADFLGTTAERISFGQNM
                     TTLAFALAHAIGRTLKPGDEVLITQLDHEGNRGPWLMLQRSGVVVREVPLLPTGELDY
                     TALRDLINARTRLLALGCSSNAIGTVNNLRLARKLTREAGALLVLDAVHYAPHFAIEV
                     SELDPDFLICSAYKFYGPHVGILYSRPGALEPLATDKLIVQEDAAPYRIETGTLNHAA
                     IEGVGAAVEYLAGFGRGASRREAVHDAVSAIAAYEHGLGRYLWSALEEIAGITLWGVP
                     FTAAERAPTVCFTLAGVDSAAIARRLGAVGICVWDGDFYAPRPVEVLKVPGRSLLRVG
                     FSMYNTREEATRLLETVQQMARDK"
     gene            24404..>25447
                     /locus_tag="VFB37_01625"
     CDS             24404..>25447
                     /locus_tag="VFB37_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HZO21172.1"
                     /translation="MKSVNDVRIGPRLLFSFLIVLGCAGVIAAVAIVQLARVAQDADV
                     IATQALGSVYRVSSIGANAAQSRAAALEILTQLQLNFTGGADGARRALVDVDRQMQVN
                     VAAYDKLIRTAEQRALWEDAAARWQAYKHEQDRSIAVAGDGLPEDAQKILIGLAEVKF
                     GAFVTAVQRLMDFSNADAESARLAANAAAVNGRHLIFVFLILAVLVGCTVAVLMTRAI
                     TIPLQATVSLLQEIGAGRLDNPIQVKRSDEVGELLSGLAATQSQLRERTTAERRRAEE
                     DRQRAAEDRQALQEVQQVVADVVDGKLERRLALEGKSGFTHQLAESLNRLIDNVAGMV
                     KGVGHLVASANGGD"
BASE COUNT         4501 a         8097 c         8253 g         4596 t
ORIGIN      
        1 tctttgcccc tggcaacagc aatgacgtgc ccttgacccg tgagcatatt tacgcgcggt
       61 gatcccatta ccgcgcagcc ctcgaagctc tgatgcgaac ctgtgatgtc ctggtcgtcg
      121 gcggagggcc ggcaggctcc tcggccgccc ggcagttgaa gctgggcggg gccagcgtac
      181 tggtgctcga caaggagcgc ttcccacgcc tcaagctgtg cgccggctgg atcactcccg
      241 aggtggtgcg tgacctcggg atggatgtcg cctcctaccc gcatcgcttc ctcaccttcg
      301 agcgattgca cgtgcacctg aagggactgc acttccggct ctcgagcgtg cagcattcca
      361 ttcgtcggtt cgagtttgac gcctggctgc tggagcgatc gggcgcggaa gtcgtacagc
      421 acaccgtgcg caacatccgg gcacaggccg gcgagtacat cgtggatgag ctcttccgct
      481 gcagatatct gatcggggcg gggggcacgc gctgcccggt ctatcgaacg ctgtttcgcg
      541 agctgaatcc gcgtgccacc gagctgcaga ccgtgaccct cgagcacgag atcgagtacc
      601 cgtggaagga tccggattgc cacttgtggt tcttcgagcg cggcttgccg gggtattcgt
      661 ggtacgtgcc caagcagaat ggctggctga acgtgggtgt cggcggcatg gccgagcgca
      721 tcaagagtag ccggggagac atcagggacc actggtccca tctgcagcag accctggagc
      781 gctcactggc ccccggcgcg aactacgagc ccaccggcta cagttatttt ctgcggggcc
      841 gcgtggaagt cgtccggcgc gacaacgcat tcatcaccgg ggatgccgcg ggcctcgcga
      901 cacgcgatat ggccgaagga atcggtcccg cgatccgcag tggacagcgc gccgctcgct
      961 cgatcctcga gggcgctgcc tacagtttgc aggacgtcac gggcgcgagt ctcgggggtg
     1021 gttgggtcag ccgggccctc gactgggcga tgacgcgggg ccatgctccg cttcgaacac
     1081 cagcagtgca ttcgccggca aagtgactgg ggctgaccag gcgcagccgt gcaaaggaac
     1141 gggcacggtg acgacccggc cccagttgcc ggccacttga ggattgatcc agttgtcata
     1201 ggcgtcactg ttgaatgctt cgcgccacga gccgccgagc ggaaagccga tccggtagcc
     1261 gtactggtgt gagttgcaga ggctcgccac cacgagcacg tcgcggccct gcgcgggaac
     1321 ccagcggtga aaggcgagca cccgatttcg atcgtgcacg tgcaccactt tgaagccttc
     1381 actcctcaat gcatgaagct catgacgcag cgtaatcagc tcgcgtgtga atcgcaggaa
     1441 gtcgcgcatc gcggggtccg gagcctccag acccggccag tagagcagca gctcaggaag
     1501 atcgagcacg tcgtccgacc actgcttgtc ctccaggaac tcctgaccca tgaacagcat
     1561 cggaatgcca ggagccgtga gcacgagtcc cgtggccacc cgtgaacggc ttcgcgccca
     1621 ccaggagcgc gggttcgcgg gatcggccgc gcgggcaatg cgctgcatcc ggttgtgatc
     1681 catgggatct ttgggccgca gcacgagatc gtgattctcg atgtcctgca ccccgcgcca
     1741 caggtcgcgc agatagctgg gcgccaggtg atccgccagt acggacaggg gtagcggctc
     1801 atcgtctgct cgggcggccg tggcgagaac attgcgcaat gcgatccgta gcccgtcggt
     1861 gagcgtggta tcgaagcccg caccgccatc gcgtgtgtcc ttcacgacgg cgggattcac
     1921 gggccagtat tccgcatgca gaagtaagcc ggggcgcagc gagcgcagcc gcgcggtgag
     1981 ctcctgacag aagtcccagc cacggccata gccgcagcgc tcgatcacgc tcacctcatc
     2041 gaaccgaaag ccatcgaccc gatactcctc gaggaagaag cgggcgttgt cgatcagaaa
     2101 acgccgcacg ccctcggact tgaagtcgaa gacattccca ccggcccagg tctgatcgga
     2161 gaagaacaac gagctccagt agcgcggcgg atcctcgtct gcgcctcgtt gacgatcgaa
     2221 gaaccacagg ctctcggctc cgaagccgcc gccggcgtgg ttgaagacca gatcgaagat
     2281 caccgcgagg ccatggatgt ggcagacatc gatcaaggcc ttgagctgat tgacctcacc
     2341 ctgcaggtcc gtgagcgcaa agggctgcaa gcctttcgca gtgagcagct gattcacctg
     2401 cgagcggtag ggggcgagcc tctcatcccc aacagcgaag gccggctccg gcgcaaaata
     2461 atccgtcccg ttgtagccga gactgaagca ccccggaaat tcctggatcg gcaggagctg
     2521 cacgacgttg atgcccagct cgacgagata ggggaccttg agcagcacat cgaggaatgt
     2581 gcctggatac ggtgggcagc gtggcgtgta gaacgctccc acatgcagct gatagagaac
     2641 gatgtccggc agtgctggcg cgacgaagtc cgtggcgtgc caggggaacg tcggacgccg
     2701 gacgatgcaa taccactgtg ccccgcgaat ctcgcgcgcg taaggatcgc gcttgtatcc
     2761 gggaccgtcc tcaccggtga tccagaactt gtagcgctgt ccttcgagga cgcccggaat
     2821 gaatccgcgc cagtgaccct ccgaatcggc ggtcagcgca ttctccgcag cgggggtgaa
     2881 atcattgaag tcgcccacca cgtgaaccgc ctttgcatgc ggggcccagg tgcggaaggt
     2941 cgcgcctccg tctaccaggt tcgcacccat gggggtgtcg gggaagcgtg cgctcacgca
     3001 ggcagagtga atcgatcggt gacgacgagc tccatgggga catcgtgcgg ctgcggatag
     3061 atcgtcggga gctgcgcgtc ggcaaagccg atgccgatcg ttttcgggcg gggtgctgcc
     3121 gctgccagag tccggtcgta aaagccaccg ccatagccga ggcgaaatcg ggcgccgtcg
     3181 aatcccacga gtggaatgac caacaattgc ggcgtgacgg gcgcgcgctc gggtggcacc
     3241 ggaatgtccc acagtccacg gcgcatcgcg acccccgggt gccagcgcca gaactccacc
     3301 ggcgccgctt tctgtaccac gaccggcaga cccacgatgc caccggactc gacgtgccga
     3361 cgcgccagcg cgcgcagatc gatctcgccg cgaatgggcc agtacagacc cagcaccggg
     3421 catttgctcg catcgatgtt ctcgaacagg tactcgagaa tcggtgcgct caggctgagg
     3481 cgctcctctg ccgatctttg cgcgcgccgc gccagaagct cttcgcgtct cgccttgcgc
     3541 cagagcttca catcctccca gcgctcggag ggggagggtg gggtagtctc gaactcatgc
     3601 agcagacatg caggggagct gtactcacgc gtttcgtcct tgcccttcat cactgcactc
     3661 ttctcaccgt catggcgcat ggtaagccgc gcgcctgctc accgagcaca ggaatgcgac
     3721 gggaccttgc cggccgcaag cgggtgaatt aaatgctcgc agcgtggcgg cattactaga
     3781 ttcaaggtct gaaatagact gtaatagtct gaaattacta tagaaccctc gcgttgattg
     3841 acgcctgtca gctgcgcgct cgaggtgcgc tactgtgacc agttcgccgg cacttcgaaa
     3901 ggcaccttca cggcggggca gctcttggcg cgctgcgcgt tggtgtgctg caggtcgctc
     3961 acccagcact cgtcgaacac cgagcagtag caggctctca ttttgatctg gatgcgagcc
     4021 gcattgaggc gctgccagat ctcggcattg gcctcgctgc gatccagggt caggaaagtt
     4081 tgggagtcac cgggccgcaa taccaacggg gcgacggcgc ccaggctcac agtgccgtga
     4141 ggcatcggag cagccctgtc gcctgtgtcg gaatcgagcc cacagcagcg cgtgagcagg
     4201 tcaagggcag aggtgacggg ctggttttgc caccaggcct cgaatgtctc gatgcgcgcc
     4261 ggcccgactc cctggttcat gagcgtcagt gagatgcgct cgtggccctg agaatccctg
     4321 ttggccgtgg tgtacatcaa gaagggccag gagttcgcgg cgaccaggcg atccatcgcg
     4381 tgaccgtgct gtatggcaac ccccagagac acgactgaca cgaacattgc agacaacccg
     4441 agtatcaggt caagccgggg atttccggtg cggtggtgcg cagtgtggtg ctcggcggtc
     4501 atccccagtg ttacctcagc gtcgtgggct tccctgcggg ggtccaacgc gcgcgcattc
     4561 tgctgatgca tgagcagatt gtaatgctgc gcagcatacg tctgcctccg cgcgggtcaa
     4621 tacggtatgg cgggttccac ccgggattcc ggtcctaaaa gacagaggcc ctgtgaggct
     4681 tcggtctaag gggagcgcga aatgaggact ctgctactgg cggctctgtt cattggtgtg
     4741 gtcggcctgg tatcggccta caccctgcgc atccgtgccg aggcgatcgc gacgtcgcag
     4801 catctggtgt atgcagccat gggggctcct cgcttcccag gcggttgccc gccggtcgtg
     4861 cgcgtgacga ctctcgacct ctgcgcattc taacgacctg tccggctgag tgccgtccgt
     4921 cggcgatccg gctccccccg gccgcgctct cgtgcgcctc aagggtgcgt gcgaccggtt
     4981 tcggccggac gctacgagcg ggcgccagaa gctgcctggt agagcccctt cagttcgccg
     5041 cggccgcgcg agcctgagtt tgctcgtaga gatgcacgtc ggcagcggca cgctgcatga
     5101 gatcgaacat gacctcatga gtgagcttgt cggttctttc gaccagcttg ccgctgatca
     5161 ggtgccgact ccccgaaacg acgccggaat catagtcctt ctcgagcaaa accctgccgg
     5221 tggcatcgag caggctgatg cgaagcgtca tccgtacttc gggctttacc tccactccct
     5281 tagggtgcgg aaatccgtat tcgaagttct cgatctgggg acgaagcgca atggagtatc
     5341 gactggccgt cgtgaagtcg tcgctttcgt cggcgccggc tgaggcggcc ttcgagaaga
     5401 ctttgagggc aatctcgcgg accatgaggc cgacgggtac cgtcaacctc agtcctgcgc
     5461 ccgtaaggct cgtcgatccc gccgttatcg aacggtcatc gtccgtccgc gtcgtgtaaa
     5521 tcagtatctt gccggtaatg cgatccgggt atgggatggg tgtgtcgggc acgtcttcgg
     5581 gccgataggc caacgtctcg gcacagccgc acaggaagag tgcggcgagc atcggagcaa
     5641 cacgcaagaa gctcatggtt ctcctctcgc ctgttgccag aagcaagcct cgctcccgac
     5701 ccgcgcttct gcagcctctt tgaccttcat tttgaagggg ttgaggcggc agagcagatt
     5761 atccgcccag cacagatcac caacttgcca gagtcgcgaa gcctcagctt cccgagtcga
     5821 tggtcaggag gcaccggagg cggcggagaa cgaccgcgct gaaagccggt tctgcgactc
     5881 tcgcgaagcg agccggtaag aagacgaggc cctctcgggc tgcggcgctc catccaatca
     5941 gaaggctgcc gattcaatgt tgctcgctta tacttgtccg tggaggtgtg gccgagcggt
     6001 ttaaggcacc ggtcttgttc caaatcgagc gctgctgcgg aaacgcggca gatgtaagga
     6061 gtcaaattcg gcgaagcccc tgggcaaccg aggtgacgcc gagcgaagcc gacatcggtc
     6121 ggaacgtgta gagacttgac ggctcccacc tgtagcttcc gagcttggtg aagacaaagt
     6181 ccagaccccg aacagatgct gtctggtggc gaaagccatg gcgggtacgg aaaaccggca
     6241 acggggcaac ccgttcagca gttcgaatct gctcacctcc gccatctgac atcgaccgcc
     6301 cacatcacgg gcatcgaggg cacgccagtc ccgttggtgc gggctgtacc tcagggctca
     6361 ctgcggaaga cttcctggcc gccgacgatg gtacgcagca cgcgcgtcgc agcgatttcc
     6421 gtgggcggga ctgcagcggg atcccgatcg atcacgatga agtcggcgag ctttcccgcc
     6481 tcgagcgtcc ccacgaaatg gtcttcgtgc agctcgtaag ccgcgttcgc ggtgatcgcg
     6541 cgcagcactt ccttttgcga taggccggag tcagtgccca gccgcccggc gtgctggggc
     6601 ggcccgccgg ggcggtccat acgcgtgaca ccgacctgca gcgcaaacca ttcatcgagt
     6661 ggatcgacgg gccagtcact tccgtacgcg atgcgggcgc ctcgggccgc gaggacgccg
     6721 gccggctcga tgagcgcgtg gcggcgcggg cccaacgtat cgcgcgcgcc ttcgatgctg
     6781 tcgaaggcgg gcttctccca ttggaaggag agcactggta tggcattgag ctcagcaaag
     6841 cgcgggtagt cggccgggtc gaccaactcg caatgcgcca aggccggccg cacatcgcgc
     6901 ccgggcaggg cctggcgcat ttgtgcaatg ccatccaggg ccgctcgcac cgctccgtcg
     6961 ccatcggtat gcaggtgcgg atcgatgcca gcctcgccga gcgcgatcag aatttcgcgc
     7021 aaaatcggag ccggaaaata cacgtccggc tcgcgcttgg cggccggctc aaatttcgga
     7081 tgcgcagcgc tgccgtgatt ctcgtaatag ggttgcagca gcgccccggt gttggccggc
     7141 gccgtgatga ccccatccat gaagagcttg gcgttatgaa ccgagatcga cggtcgcggc
     7201 cgcagtggcc cttcgtcgta ctgccgggcc agggccacaa cgcgcgttac tgcgcgtctg
     7261 gcggatggag catccggagt ctgttcggga cgaatccggg gagcgaaatg tgcgcgcgcc
     7321 gtgagcccac ccgccttctc gatggcggcg aaagcctcga tgtccggtgt ctcagcgacg
     7381 gcatcgagaa aggcagtgac accttgacgg cgcatggcat ttagcgctgc ccgcacggca
     7441 gcgagattct ccgccggtgt gggcttgggg actcgctcca ggaccagatt ttgagcggca
     7501 tcctcgagga agccatccgg ttctccggaa gcataccgcc cgatgcgccc attcgccgga
     7561 tccgatgtct ttgctgtgat cttggcgagc gcgagagcgc gtgaattgac caggctggaa
     7621 tggccgaaag tgtcgcgtac cacgatcggt ctgtcggtct tgagcgcatc gagcgtggcg
     7681 cggctcaccg tcgcgccctg cggacgcaga ccgtagcgaa accagttcac cacctcgaac
     7741 caggcttcgg cgctgttgct gcggtgctgg tcgaggcagg cttgaatgtg tgcctggaac
     7801 tcggcaatgc tgagggactc gtagttgagc gagcatttga ggagcgtaag accgccttcc
     7861 accgggtgca tgtgcccgtc gatgagacct ggcataacgg cgcggccttt gaggggcaca
     7921 agccgggtgt tgtgtccgac atagcgcctg gcgcctgcat cactgccggc gtaaacgatg
     7981 cttcctcccg ccacggcaag tgcctgcgct acggtgtctc gtgcatccat cgtgtagatc
     8041 acgccgccgg tatacaccgt gtcagctgca cccggtgcgg cctcagccca gcaggcaagc
     8101 cagaacagca gcaggccgag tccaagactc gagctcatcc attggcgccg cccggaaaaa
     8161 ttacttttca cgtcgatggt ccttcacaag catcgaatag ctgtcgtcgc gcgcccgata
     8221 aggtctctgc aactgtgcag gcagcgagca gcgcgccgga acttgggttg aggccgatag
     8281 ttaaccagta tcggccagca gcgcggagcg cacagtcatg gatcattcgc acggtttcaa
     8341 acgacgacag aatttcatct taccgttggc agtggctctc gccgcgagct ggaccgtcgg
     8401 gtatgccgct gatccaacgg acgatgcaac ggtcaagtcg caggtagaag ctgcgctcaa
     8461 cggcgactcc aacctcttcg ctaagcacat cgacgtgtct gtgaagcatg gcgtggtgcg
     8521 tctgagcggg ttcgttcaga agcagactga actcgaaagg gcgaagaagg atgcggcagg
     8581 cgtgcctggt gtcatgtcag tcaaaaacga attgacactc aagcataacg aaaccggcgc
     8641 ctctcactga aagatcgccc gagcagccct ccgcgggcag cgcttcccac cttcaattga
     8701 ggggtcgcgg ctcgcgtgtt cggctattgc ggtggcgcat cgccgccgcc ggggacaagg
     8761 gcccgtggtc gggttggctg gtagagggtg agcgggttgg ctggtagaga gcgtccggta
     8821 aagccctcca accaatcggg ccaagcccgt gaaccccggg gattatgaca actctgactg
     8881 ctcgcgcttc gagacctcgc taggattcgg ctcctcacga agaacaaagg gctggagccg
     8941 tgagccagaa gccaacgcca ctgacggacg ccgtcgtttg cagggatgag aaggggaaca
     9001 tcgtcatcac cgggaaggtc aacggccgcg aagtcgtcca caccgtcacg ccgagaccca
     9061 agagcgtgca acgccagctc agcctgtaca aaaagctctt tgcggatctt ggctgaggct
     9121 cacagcagtc tcagctgccg aatgaggatc tccgcttcgt agttcagtag tggcagtccc
     9181 tgcgcgagac actcgcagat ccgcgctcgc tcggctgctc tgacctggga cgcagagacc
     9241 tgccactcgc ggacgatcat ggtgggcagt cgcccatcgt cgcgatagag atctctgatc
     9301 cactggtaca gcgggcgcag tttcggcgac ttgacccacc acggaagcgc atcaggcagc
     9361 cataacctcg cgctgttcaa atgttgccgg aagcgtccct tgggtgcgcg ggtttgtcct
     9421 acgtaacgga tcagacgcag atctctcgga tccgaaagcg tgtagatcgc agtgcccgtg
     9481 ccgtcgccgg cggactcgtc atacagctcg gcggcgatct ggcgcctgac ggcctgccga
     9541 atctgcgctt tcgtaggagc ggccgcgctc agctcggctg agtcctgcag agcctcgtct
     9601 gacaaatgca gcgccacggc cggatcgtag acatcagtgc taacgcacgt ccacccagtg
     9661 acggctcggc cccaacactg tatgcagcgg gccctcgcgt cggggcggct ccataacccg
     9721 ggttggtcac ctcatgcgtc agggggcagt cgtgaccgga tacgcggtgg ccgcagggaa
     9781 tctggcggtg gtgacagcat tgttctggtc acaggcgccc ccgatcgcat ccccggcgcc
     9841 ggtcgccttc gggcccgcag ccgagcccca gtagtcatag tttgcggcga gcggcgcccg
     9901 cggaggagga gtgccggcgg ggccgccgat aatgtcggcg agcgcaccga cgttgagcac
     9961 cccgcaatgg gcgctcgggc ctgggttata aggaccgtgg ccaccaccgc cgtcgccagt
    10021 atgcagtgcg agcgtggggc gattacggtc attgccgaag aagttgttct gactgaaggt
    10081 ctggaagctg cccccgaatt ccgtgaacgc atcgggcgac cactgaatga tgacgcccgg
    10141 tccagaattg ccgatagctg agttgtgctg gaacagcgat ggggggcctg gggtcacctg
    10201 gaagccggcc tgcgagttgt aggcggccgt gttgcccgag atctctccgg gcatgttggc
    10261 atagaagccg atgccagagt tggtggcaac gttattgcgc cattggatgt tgctggctcg
    10321 gatcgccact tcacacgtct tgcattgaaa gccggtctgg atctggaatc ctccgccgga
    10381 tcccatcgcc agattgttct cgaacagcac ctgtccggga ccgaggtcca tgttggcggt
    10441 ggcggaataa ctgaaaccgc cgttgccata ggactcattg tccgagaaaa gcagctgggc
    10501 ggtcggagcg catacgggtg agctcgtcgg gcactcctgt gtagtttccg tccgaccgga
    10561 gaacacgaat cccgatccat cgccgatgtc cacgttgccc gaaaccgaaa tgttcctgga
    10621 cggccccaca ctcgagacat cctccatggt ctggtaatcg atctcgaggc cgaccgagct
    10681 gccgcccgtg agcgtaaagc catgacctgc ggaaccgaaa gccaccccat cctggaggat
    10741 ggccacattg gccatgctga tgccgcttcc ctgcggcgga gggcttccgg cgatcacggt
    10801 cactgaagca ccgctgaccg agtagatatg cagcgacttg gtgatgcaca cgaggcagcc
    10861 ctcgctgtca ttcacgaacg tctgcggtcg ctcatcaccc gggccgctgt aatcagcact
    10921 cccgctgacg ttaccgtaat ggccgggacc cacatagatc gtgtcgccgg attgcgcgtt
    10981 ctcgatcccc tggctgatgg agcgacacgg attactggcg gccccgcagc tcgggctgtc
    11041 cacgccgttg ttggcgaccc agacccgcgt ggccgcggcc gaaacgttgc tgaggagaag
    11101 agcgaacagc agcaaaccca gtggcccgag ccgggagatc gcgaggagac gcatgatcac
    11161 tgcctttggc tccgcgggtt gcttatgttt cttcagcgga gagcgatttg actataagtg
    11221 caggggctcc tggaatcaaa gctgcacagg cccgttagat gtgcggtgcg tcacagttct
    11281 gcaaggcgca acgcggggtg atctgtctta ggtccgtcag atcgtcagag cctcggcttg
    11341 caagcccggc actgaccgcg acgccgagag aaaaattgcc agtggcgcga gggcattcgc
    11401 gcgcaacctc tcgtcagcgc gcgcacgagc cacatttgct tacaagagac actgctccac
    11461 ggcggcggcg accggccata ggcgctcgtc cgcatcacgc aggccgaata gcgtgagacc
    11521 caccggcgcg gtccccggct catggcaggg cagggagatg gcacagccgt cgagaaaatt
    11581 gacgacactc gagttccgca ggatgagcgc gttcgtgcgg acatagacat cgtcggcgct
    11641 cagctccgac aaggcggggg ccgacacggg gaccgtcggc atcacgacgg cgtcaaagtc
    11701 gatcaggcgg cgcgagaccc gctcgatgaa agcgactcgc gcgtgaagaa gctcgatata
    11761 gtcggcggcg tcctgttcac gcgcgcgcat gatgcgattg gccaccctgg ggtcgtatcg
    11821 tgatgcgtcg ctgttcaggc gcgtccggtg cacggcatac gcttcggccg ccgcgatgcc
    11881 gcctttgcgg ttgaggccgg gcagctcgcc cagctccggc acgtcgacgt ccgtaagcga
    11941 tgcgccagca gttgacaggg cccgcaatgc ccgctggtaa tccgcggcca cttgcggcgc
    12001 gagtccgtcc atgacgtagg acgtcaatac gccgagtcgc agtccacgaa ctgctgctgc
    12061 ggaatgccga tccacgccgc ccgaggccag ctcctggagc gctgccgagg ggatatcgcc
    12121 cgacagcacg ccatcgaggg cacggcagca ggcgaccgag cgagccaggg gcccgacgga
    12181 atcaagagtg ctcgagagcg ggaagacgcc tgacttcggt acgcgatgcg cggtcggttt
    12241 gtagcccacg attccgcaaa gagcggccgg gatgcggcac gatccaccgg tatcggttcc
    12301 gagcgcaccg aaggccatcc tgtccgtcac cgaaacggcg gcgccggagg aggagcctcc
    12361 ggggatcctt cccgtggcgc gatcccaggg attgagcgga gtgccgtagt gcgggttgag
    12421 tccgagaccg gagaaggcaa actcggtcat gttggtgcgg ccgatgacga cgaaaccggc
    12481 agccttcacc cgtgctacgg cggggcagtc gagctgcgcc gccggcgcat cgcgcagcac
    12541 ggcggaggca gaagtggtgg tgtcgcccgc gatgtcgaac aggtccttca ccgagatcgg
    12601 aatgccggca tagggactcg atgccgcgcc atgccgccgc atctgatcgt ggaattcggc
    12661 ggtggccagc gcctgcgcgg cgtgcacctt cagaaaggtg cgcgcacctt cgccttgctt
    12721 gtccgcaatc cgctccagac actctgcgac cagcgcacgg ctgctcgtgc gtccggcggc
    12781 cagcgcttgc gccaggctgg cgagagtcgg cggggagtcg ctgctcatcg cactcagcgc
    12841 ttgcggtata cggtcttcgg cagtacgcag agcatctcgt acagcaggtt ggctgccagc
    12901 agcgaggtgt tgccggtact gtcatacggc ggtgcgacct cgaccagatc gccccccacg
    12961 atctgcaggc tgcgacagcc gcggatgatc tccagtgcct gcggcacggt gaggcctgcg
    13021 atctcgggag tgccggtgcc gggcgcgaag ctcggatcga tcccatcgat gtcgaacgac
    13081 aggtaaaccg gtccgcggcc gaagcttgcg cggatctcat tcatgagcgg tgcgagcgag
    13141 cgcagccagc agtcctccgc tgctacgacc cgaacgcctc gctcgcgtgt ccagtcgaaa
    13201 tcgctcgagg aatagccggt gccgcgcagc ccgatctgaa cggcgcgccg gcagtcgatc
    13261 aggtcctcct cgatggcccg tcgaaatggg gtgccatggg cgagctttga gccgaacatg
    13321 gtgtcattcg tatccgaatg cgcatcgaca tggatcaaac ccacgggacc gtgcttgcga
    13381 cgcatggccc gaaggatcgg caacgcgatg gtatgatcgc cgcccagcgt gagaggtacg
    13441 caatccgtac gcaacacctc ctcgaacgcc cgctcaatga tctcgatact ctcggcgaga
    13501 tcgaaggtgt tgatcgccac atccccgaca tcggccacct gcatgctgtc gaagggagcg
    13561 gcaccggtcc ccatgttgta cggccggatc agacacgact cggcccggat ttgtcgaggc
    13621 gcgaatcgcg cacctgagcg gttcgaggtg ccgatgtcga gtggcacgcc gatgaagcag
    13681 gcatccaggc ccacggcgct cgcggccgtg ggcaggcgca tcatggtcgc cgggcccgcg
    13741 aagcgcggca tggaatttcc gcccagcggc tggttgaagg agatcgcgct gccggggctc
    13801 tgtacgggtt tgtccatcga ggcctgcctt gtgcccagag aaaagaggtc cggctgcatt
    13861 gtaaccggcg gccgcgaaaa tccttattga acggtcgata aacagtggca gccacgatgc
    13921 gagtggtcta tcctcgtgct gcctggtcgg ggggatcgat ggggcgccaa ccacgttacg
    13981 atgtgctgtt cgagccggtg cgtatcggcc cggtgaccgc tcccaatcgc ttctacgccg
    14041 taccgcatgc cagcggcatg accaatacga tgccccgcat gcgcgcgggc tttcgggcca
    14101 tgaaggcgga gggcggctgg ggcgtggtct gcaccggcta cgtctccgtt cacccgagct
    14161 cggacgattc gccgctgccg tacgcgaccc tctgggacga caacgacatc cgctcgcacg
    14221 cgctcatgac cgaagccgtg caccggcacg gcgccctggc gggcatcgag ctgtggcatg
    14281 gcgggggctc ggtctccaac aagacgagcc gctttccgcc gctctcgccc tcgggcctct
    14341 cctggatggc aacccacgtg ggcttcatgg gcaacgcgcg ctcgaaggcg atggatgccg
    14401 ctgacatccg caatctgctg cactggcaac gcgaggccgc gcgcaaggcg cgcagcgccg
    14461 ggttcgatat cgtttacgtg tacgccggca tggggtacct gccctatgaa tttctgcttc
    14521 ccgagtggaa tctgcgtacc gatgcctatg gcggcgctat tgccaatcgc gtgcgcctgg
    14581 tgcgcgagct gctcgaggtc acgcgcgatg cggtgggcga cacctgtggg gtggccctgc
    14641 ggatcagcct ggaggagctg cgcagccggc gcagcgagca tgcgccctcg gaagcccacg
    14701 aggtggtcga gctgctgtcg gactccaccg atctgtggga cgtcaagatg gactcgagcc
    14761 ccacggattg cggcccctcg cgcttcacgc ccgagggcag ccacgagccg gtgatcgatt
    14821 tcgtcaagcg catcaccggc aagcctgtgg tcggagtggg gcgattcact tcgccggaca
    14881 ccatggtctc gcagatccgg cgcggcgtgc tcgatctgat cggtgccgcc cgcccctcga
    14941 tcgccgatcc cttcctgccg agaaagatcg acgagggccg ggagcaggac atccgcgagt
    15001 gcatcggctg caacatctgc atctcgagct ggcacgacag cgttcccgtg cgctgcacgc
    15061 agaatcctac gatcggggag gagtggcgac gcggatggca tcctgagcac tatggagcgc
    15121 cgggacgtgg ctcgacggtg ttgatcgtcg gcggcggtcc ggctggcctc gagtgcgcac
    15181 tcacactcgc tcggcggggc tactcggtct cccttgcaga ggcggccgat cacttcggcg
    15241 gccggctgcg cttcgagacg agcctcccgg ggcttgccac ctggagccgt gtcctcgact
    15301 ggcgcctgag tcagctccgt cgcttgtcca atgtcacgtt atatgccggc aatgagcttt
    15361 ccgtggagga catcctcggg ctcgagcacc agcgtgtcgt ggttgcaacg ggtgctcgct
    15421 ggacgcggat gttgtattcc acactcgagc tggcagtcgg cgatctcgag gctgcgaacg
    15481 tcttcacacc ggacgatgtt gccgcgggca agctgatcga aggccccgcg gtggtgttcg
    15541 atttcgataa ctactacatg ggcagcgcgc ttgccgaata tctggcgcag tccgtggatc
    15601 gggtgagcta cgtcaccccg gccgggcatg cctccgcatg gaccatcatg acgaacgagc
    15661 agccgcaggt gcatcgtgcg ctcgccaatg cgggggtcaa gctgcacacg ctctgccgcg
    15721 tgacccggct cgaacgcgac gaggtggtga ttgccaacct gttcactggc gaggagcggc
    15781 agattccctg tcgatccctc gtgatcgtgg gcgctcgcgc ggcgcgtgac gacctgcatc
    15841 gggcgttggt tgcccggcag gaggagctca gcgctgccgc aatcctctcc gtcgatcgca
    15901 tcggggacgc actggcccct ggggccatcg tgcacgccat tcacggcggg catcgttacg
    15961 ggcgcgagct ggacgccgag ctgggcagtg atctgtaccg gcgcgatgct ccgatcgtcg
    16021 acgaggcgcc gcttctatac gacgatggca gtgcagagca ggcaggaaaa tgaagacatc
    16081 cgaggtcgcg atcattccag ggctggagca gactttgcct tcgagctggt actactctga
    16141 ggaggtgtac cgcctggaga gagagcggat cttctgccgg gagtggctct gcgcggcgcg
    16201 cgccgaggag ctccccaacc ccggcgacta ccgggtgcag gacatcgtag gcgagagcat
    16261 cctgctggtt cgcaaccggg agggcacgct ccgcgctttc tacaacgtct gccgtcatcg
    16321 cggctcgcgg ctgtgtcgtg ccgatgggcg gggccccacc ggtatggcgg tgcagggcgg
    16381 gctcatcgcc ggccgctcga tcatgtgtcc ctatcatcaa tggacatacg atctcgatgg
    16441 ccacctcatc gcagcgcccc atctcggctc cgtcacaggt ttcgacaaga gtgagatcag
    16501 tctgtatccg gtgggcgttg aatgttgggg cggatttgtc ttcgtgcatc tcacgccggc
    16561 cgaggcccag cccctggcgg cgcagctcgg ggagattccc gcgcgcatcg cccgctatcc
    16621 gctggccgag ctgcgaatcg ggcacagcat cgcctatgag gtcaaggcga actggaagtc
    16681 catctgcgaa aactataacg agtgctatca ctgcggcggg gtccatcccg agctgtgctc
    16741 cgtcgtgccc gccttcagag aggccggcgg cggcgggctc gactggatgc gcggcattcc
    16801 gcatcggcaa ggggcctaca cgttcacgcg cagcgggacc accgcgcggc ggcctttccc
    16861 gagcctcaac caggacgagc gcgagcgcca caagggggaa gtgatctatc cgaatctgtt
    16921 cgtgagcctc gcctgcgatc acgcggccgt gttcatcctg cagccgcgcg ggccggagcg
    16981 cacggatatc ctgtgtcact tcctgttcga gccgcacgag atcgcccagc cgggcttcga
    17041 ccccgcggat gccgttgaat tctgggacgt cgtcaatcgc caggactggg agatctgcga
    17101 gtcggtgcag cgcgggatgc gcagccgggt gcatcagttc ggctactacg caccgatgga
    17161 agatttcaat ctcgacatcc gccggtatgt tcacgagcgc ctggatccgg ctccggaccg
    17221 cccatgcgac gatgcgtgag ctcgaacggc ctcatcgctc aagtcgattc gctcaccgct
    17281 cccacgcccc tgtgggcgct cactcaccgg gtgcgctggc gcatctactc cttcctgttc
    17341 gtcttcggct gcatcgccta tttccagaag ctggggctga ccgtcgcagc ggaccgcatg
    17401 atgcccgacc tgcacatcac gcaggcgcag ctcgcgtggc tggagtgggc gctggtgctc
    17461 ggctacacgg ccgcccagtt tcccggaggc attctcggcc gtcgactcgg tgcacgccgc
    17521 gccttcgtgc tgatcagctg cttcgcactg ctcggcaccg tgctgactcc tttggcaccc
    17581 gagctgctgc acggaatgag cctcatcggc gtgttgcttg cgctgcaact cgtcgtgggg
    17641 ctcgcgcaag gtccgatctt tccggtgagc tcgggcgtca tggaggcctg gttcccaccg
    17701 gagaagtggc cgctcgtcca gggcttgcag tcagcgggcc tgcagctcgc ggctgccctc
    17761 accgcaccga tcgtcgccta cctgatgacc ttcctcggct ggcagcaggc gctcttctgg
    17821 cccgcactgc ccgccgtgct ggtgatcgca gcttggggat ggtacgcgcg caacaccccc
    17881 accgagcacc gcgccgtcac cgcccaggag cttgccgagc tcggcccgat tccgcccgcc
    17941 cagccgcaag ggccgatgag ctgggagcgg ctgcgtcggc tactctcgaa ccgatcgatt
    18001 ctgctgctca cctgcagtta cgtctgcatg aactattcgt tctacctgat cgccaactgg
    18061 tgcttcctgt atctcgtcca ggagcgccac ttcaacgtgc tcgagggcgg ctggctcgcc
    18121 accttgccgc cacttgccgc cgcggtcggc gccggcctcg gtggcaagct cgtcatgatc
    18181 gcctgcgcgc ggctcggcaa tcgctggggg tttcgcgccg tgcccctgct ggccctaccc
    18241 ctgacgggag tgctgctgct cgccaccgta catctcggca acccctatgc ggcggtgatc
    18301 gcactcgcgc tggcgtatgc ggcggtggag ctgaccgagg gcccttactg ggcaggcacg
    18361 atgttcattg cacgcagcga gacgatgccg gcgacgggcg tgctgaacac gggcggcaat
    18421 ctaggtgggc tgatcggtat tccgatcgtg gggtatctgt ccggctccgg tcactggaca
    18481 ttcgccttcg gactcggcgc gattttcagt gtcctcggtg cactcgcctg gctgggcatc
    18541 gatgccgagc aaagcttcga tcggcccgcc gggcgcactt gacgagtgcg cccggctatt
    18601 atacaaccat ttaataattt atatggccat tcaaattggg catccagcgc ggtctcatgc
    18661 cggatttccg taaacgcaac cccctgatcg ctgcggccat tgcgaccgca ctcgcgggca
    18721 cccggctcgc tcatgcggcc gatgccgacg cggcacccac cgcctccaca ccagcgccag
    18781 tgctgcagga agtggtggtc acggcgaatc gccgcgagga ggacgtgctc accgttccgt
    18841 acaatatcag cgcgatctcc gccgcacaga tccaggatgc gggggtcaag gatctcgaag
    18901 gcctcacccg tatggtcccg gggctggtcg ccgccgatct cgggccgcgt gtgaacagca
    18961 ccaacagcac gctgatcatc cggggcctga atgcctcgga cgaaggctcg aactacttcg
    19021 gtccgaacct cgccgtgccg ctggtctcga cctacctcga cgatgtgccc ctgttcgtca
    19081 atttcaatct caccgacatc gagcgggtgg aggtgctgcg cggaccgcag ggcacgctgt
    19141 acggctcggg tgcggtgggt ggcacggtaa agctgctgcg gcacaagccg gatctcgggg
    19201 tgttttccgt cgatttcact gcggatacct ccggtaccga gcactcccag ggcccctcgt
    19261 attcgttctc cggcatcgtc aacgtaccgg tttccgatac ggtggcgctg cgggcgagcg
    19321 ccggcatgac gaagattacc ggcttcatcg atgcgagcca cgtggcgcgc ttccagccga
    19381 acggtcagcc gttgctggcc gatccgtccg cgcccctgac cagcgactat cagtacacga
    19441 cgaagaagga tgtagacagc gccacgagcg agtacggccg actgtcgatg ctgtggaagc
    19501 cctcggaagt cttgagcgtg ctgttcgact acacgcacca ggaagatcac tccggcggct
    19561 tctctgccga gacggtcaac ttcaattatg cgaccggcgc gccgattccg attcagccct
    19621 acacgaccac gaccccgctc ccggagcagt ccctgaatcg cctgctcgac atcggcgcgc
    19681 tcacggtcac ggccgatttc ggcttcgcga cgctcacttc ctcgacttcg tattacgaca
    19741 atcgttacaa cgacgtgatc gacgtctcct ccacgcagca gtacttttcc ctggcgaacc
    19801 cgtactatta cggcggctat ccgcgcttcg cggcgttcaa cttcgattac tcgaaggacg
    19861 aatctctggt ggaggagctg cgcctggtct ccaagactgg cggggcgtgg gattacatcg
    19921 ttggagcgtt ctttcgcact cagaaggagg ctctgtcgga tcccgagatc tggccgggcc
    19981 tggccagctg gtcgcacttg cccggctccg ccaatgcgta caactactat gccgggacga
    20041 ctttcaacac cttcgcggat gtgatcgcct cgcgcgacgg cagcgatcca gagagcctga
    20101 agccgaccga tctgttctac aacaacatcc gtcacacacg attcaaggat cgcgcgctct
    20161 atggggaact cacccgtcat ctgaccgacc gctggcaggt gaccggtggc gcacgcgagt
    20221 tctggcaggc ctacgatcag gtcgagacgc agatatttcc ctttgcgggg gcgttctatt
    20281 cggacaccgg gaccgatccg ctcggggata cgaccagcga gcaggcgaag tcgtatcgca
    20341 accacatctt caagctcaac acctcgtacg agatctcgcc ggcggcgcgg gtctatgcca
    20401 cctggtcgga gggcttccgg cacggcggcg cgaattcgtt caagatctgc ccgtccggcg
    20461 cgccgcccct cgggggttgc gagccggcgg agtccgctgc gctcattcct tatgttccgg
    20521 atacggcgaa gaactacgag gtggggatca aaggggagat cggcggccgc gttcgctaca
    20581 gcacctcgct gtaccgcgtt gactggcaca acatccagct cgacgccttc gcgccggtga
    20641 ccggcatcca gatcgtcgcc aacggcaagg atgcgcgcag ccagggtgtg gaactcgagg
    20701 tgcaagcgct tgtcacgaca gggctctccg cgacgatcgg cggtagcctg accgatgcgc
    20761 acctgaccga cagcttcgtc acggcgaatt tcatcgggca gaaaggcaac gccttgccgc
    20821 tggtgccgcg tacgcagctc acggccgccc tcgattacgt gttcttgctc cctcaggaac
    20881 gtgatgtgtc atttcatatg gacgctgcct atcgaagcgc cgtcgacact tctgtgaacg
    20941 atctgcaggt ggatgccaac ggcaacactt tcgtggacat ctacgccacc aacttccgtc
    21001 ggctgagcgg ctttaccaca ctcaatgccg gcatcggctt tcaggcgagc aagcaggtga
    21061 agctgcggct gtattgcaac aacatcacgg accagaaggg cgtgacgacc tggtcggtgg
    21121 cgcatcagcc ggagcagtcc ttcgaatacg tgatgcggcc gcgcaccgcg ggcatcgagc
    21181 tggactacag cttccgctaa gtcggaccgc gcatcagggg acctgtacgg gcgggttgcg
    21241 cgccgttacg ctcaatccat gtcgggcgag tcctcctcag cggccatagc acgtcatcgt
    21301 gaggtgaccc gtcttctgca ggcaggccag ctcaaggcgg ccgaagagca gtgccgcacg
    21361 ctcacgagcg acttccccct gtttcttccc ggttggcata gcgcgagctt catagagctg
    21421 tgcctgggcc ggctgggccc ggcactcgag tcgatcgggc gggcactggc gagctcaccc
    21481 gccgatcccc gctacaggct gcagcacgca cgctgcctga gtgcgctcgg caagttgccg
    21541 gaggctctgg cgagcgcagc catcgccgag agcggggcgc tcgcggacgc cgcgcttctc
    21601 gatgccatcg gctccttcta cagcgccacg ggcgagcagc acagagcgct gcaggcctac
    21661 aaccgcgcga tcgcgctcga tccgcagcag gccgtgttct ggttcaatcg cgccgcggtc
    21721 cggcgcttcc tcggggatct cgacggggcc gaggccgact acgatcggtg cattgccctg
    21781 cgacccgagg accacgaagc ctatctcaac cgctcggagc tgcgcaggca gcgccccgag
    21841 cgcaaccatc tcgaggtgct cgaggggttg ctgggagcgg gtgtgcggca gtggcgcggt
    21901 gaagtgcaga ttcgttatgc gctggccaag gagtacgagg acgtcggcga ctatgcgagc
    21961 tcgtggcggc atctgcagag cggtgctcgc ttgcgcagac agcatcttca atacgatgtc
    22021 ctttacgatg tgcgcacggt ggactggatc atggaggcgt ttgcgtccgc gcccaaagag
    22081 cccgttggcg gttgtccgag cgctgcgccc atcttcatcg tcggtctgcc gcgcagcggc
    22141 accacgctgg tcgagcgcat cctgggcagt cattccgagg ttcatgcagc cggggagttg
    22201 aatcattttg ccgcggcgct ggtggcgggc gtgcaggcgg gccgggggga tcgcgccttg
    22261 gctcggcggg agctgatcgg cgcaacgcgg cagctcgatt tcacagcact cggggtggcc
    22321 tacctgcagc gcgcccacgc gtctactctc ggccggccac gcttcaccga caagatgccg
    22381 ctcaactatc tgtactgcgg cctgatccat cgggcgctgc cgaatgcgcg aatcgtgcac
    22441 gtcacgcgcc acccgatggg ctcctgctac gcgatgttca aaaccttatt caaagagggc
    22501 tatccgttct catacgatct gggtgacctc gcgaagtatt acgccggtta ccgtcgcctg
    22561 atgcagcact ggaacgcgac gctgcccggt gtcattcatg agatcagcta cgagcggctg
    22621 atcgcggctc aggaaaccga gacgcgccgc ctgctcgagg cgtgcggact cggctggcag
    22681 gaggcgtgcc tggaatttca tcgcaatcca accgcgacca cgacggcgag cgcggctcag
    22741 gtgcgccagc cgctttacga cagttcggtc atgcagtggc ggcactacga ggagcaactc
    22801 gtggggttgc gggctgagct tgcctcgctt ggcgtcacat ggcaggacgc cggcccccta
    22861 caatagcgca ccaggcgccg caagcggcgc ggagaccctt gatcatgccc caagctacgg
    22921 ctgcgacaat cttcgatgcg agcacgtgcg agcgctgcag ggccgacttc ccctctctcg
    22981 cgcgccgctt cggcccgcac acggtggcct ttctcgacgg gcccggcggg acccaggtcc
    23041 ccaatgcggt catcgaggcg attgccgatt gctatcgcaa gcgcaacgtc aacacgcacg
    23101 gcaactttcc ggtctccgct gagctcgatg agcggcacta tcagatgcgc gccatcgtcg
    23161 ccgatttcct gggcaccacc gccgagcgca tctccttcgg tcagaacatg accacgctcg
    23221 ccttcgcgct cgcacacgcg atcggtcgca ctttgaagcc tggcgatgag gtgctgatca
    23281 cgcaactcga tcacgaggga aaccgtggcc cctggctgat gctgcagcga tcgggagtgg
    23341 tggtgcggga ggtccctctg ctgcccaccg gtgagctgga ctacacggcg cttcgggacc
    23401 tgatcaatgc tcgaacccgg ttgctggcgc tcgggtgctc ctccaatgcg atcggcacgg
    23461 tcaacaactt gagacttgcg cgcaagctga cccgcgaggc gggcgcgctg ctcgtgctcg
    23521 atgccgtgca ctacgcgccg cactttgcaa tcgaggtgag cgagctcgat ccggattttc
    23581 tcatctgctc cgcttacaag ttctatgggc cgcatgtcgg catcctctat tcgcgcccgg
    23641 gcgcactgga accgctcgcc accgacaagt tgatcgtgca ggaggacgcc gcaccctacc
    23701 ggatcgagac cggaacactg aatcacgcag ccatcgaggg agttggcgcc gctgtcgaat
    23761 acctcgcggg gttcgggcgt ggcgcctcac gccgtgaggc ggttcatgat gcggtctcgg
    23821 ccatcgcggc ctacgagcac ggtttggggc gctatctctg gtcggcgctc gaggagattg
    23881 ccggcatcac tctgtggggt gtcccattca ctgcggcaga gcgcgctccg acggtctgct
    23941 tcaccctcgc gggggtcgac tccgctgcga ttgcccgcag gctgggggca gtgggcatct
    24001 gcgtctggga cggcgacttt tacgcgcccc ggcccgtgga agtgttgaag gttccgggac
    24061 gctcgctgct gcgagtcgga ttttccatgt acaacactcg cgaggaagcc acacgccttc
    24121 tcgagaccgt gcagcagatg gcgcgggaca aatagctttc agctttgaaa ggtagcgact
    24181 tgccgggtgt cgcaaccggg tgccggatga cggcttgccg gccgggggcc caggcccccg
    24241 ggttggggcg ccaacccgcg atcagcgcgt tagccggtgg ctggggccgc gtattggccc
    24301 ggcggggggc cgcgtaacgg cctcggctgg ggcgcgcgta cattctgtga actgattcgc
    24361 cgggccctgc tcaagttttc ccgcgccggg tcgttttggc agcatgaaat ccgttaatga
    24421 tgtccgaatc gggcccaggc tgctgttctc cttcctgatc gtgctgggat gcgccggggt
    24481 catcgccgcc gtcgccatcg tacagctcgc acgagtcgcg caggatgcgg acgtcattgc
    24541 gacccaggct ttgggcagcg tctaccgggt ctcctccatt ggcgccaatg ccgcacagag
    24601 ccgcgccgcg gccctcgaga tcctgactca gctacagctc aacttcaccg gcggcgcgga
    24661 tggcgcacgc cgcgcgctgg tggatgttga caggcaaatg caggtcaacg tcgccgctta
    24721 cgacaagctc atccgcaccg cggagcagcg tgccctgtgg gaggacgcgg ccgcgcgctg
    24781 gcaggcctac aagcacgagc aggatcgctc cattgcggtc gccggcgacg ggttacctga
    24841 ggatgcgcag aagattctca ttggcctggc cgaagtcaag ttcggcgcct tcgtcacggc
    24901 cgttcagcgc ctgatggatt tcagcaatgc agatgcggag agcgcacggc tcgccgcgaa
    24961 tgctgccgcg gtcaatggcc ggcacctcat cttcgtgttc ctcatactcg cggtcctcgt
    25021 cggttgcacc gtggcggtcc tgatgacgcg cgcaatcacc atcccactgc aggcgacggt
    25081 gtcactgctg caggagatcg gcgccggccg cctcgacaac cccattcagg tcaagcgctc
    25141 ggacgaggta ggcgagctgc tctctggcct cgccgccacg cagagccagc tgcgcgagcg
    25201 caccacggca gaacggcgcc gggcagagga ggatcggcaa cgggctgccg aggatcgaca
    25261 ggcgctgcag gaagtccaac aggtcgtggc cgatgtcgtg gacggaaagc tcgagcgccg
    25321 tctggcgctc gagggcaaga gcggtttcac gcaccagctt gccgagagcc tcaacaggct
    25381 gatcgacaac gtggccggca tggtcaaagg cgtcggtcac cttgtggcga gtgccaatgg
    25441 cggggac
//
LOCUS       DASRHN010000043         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1828423, whole genome shotgun sequence.
ACCESSION   DASRHN010000043 DASRHN010000000
VERSION     DASRHN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828423"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFB37_01630"
     CDS             <1..190
                     /locus_tag="VFB37_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21173.1"
                     /translation="EFPPVALFEYPTIRSCAGYLRSVRAGTHGAAESAPGESNDGSEE
                     REHLLLMAETLRAASDAH"
     gene            180..>3984
                     /locus_tag="VFB37_01635"
     CDS             180..>3984
                     /locus_tag="VFB37_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO21174.1"
                     /translation="MRTEAADRNSGLAIAIVGMTARLPGARDLEEFWKLLADGNEGIR
                     RLSDAELRAAGLSEERLRDPDLIRGLGLIDEGELFDAGFFGYSSSDAEILDPQQRIFL
                     EQCWMALESAGYSPEKFNGAIGLFAGCGLSAYTTRLVASGWVARSGIGFQKIVGNDKD
                     HLTTAVAYKLGLTGPAVTVQTTCSTSLVAVHMACRSLLGRECDVALAGGVTAGLEFGS
                     APTYQEGNILSRDGHCRPFDAQASGTVVSNGAAVVVLRRLEDALRDGDNIRAVIRATA
                     VNNDGSLKVGYMSPSVAGQFKVVRRALRLADVHPETVDYVEAHGTGTLKGDPIEVRAL
                     TRAYREQTARSGYCALGSVKANIGHPDTAAGAAGLIKTVLAMQHGVIPPTPHFTTPNP
                     ELELENSPFFVNNTAIPWPRAARPRRAAVSSFGLGGTNAHLILEQAPEVPHAAPDDEP
                     QLLLISGKTPTALAASCRGLAGYLRKNSQLALRDVAFTLRYGRADLKERAWFVGSRVQ
                     ILEQLEAGSDCAAGSASRWLQQARDYYRSSAAFRELVAQNAKRYAAGQIEIEKLLQSA
                     TQDGAGLRDLAVSVGDPDAGQALQLALSVMGELWRRGQVQAVAAPPGMAEGQRIELPH
                     YPFERSRYWIDTAESPRPSRDSAPVASDVSGWLYIPVWRQCAAGPSSGRPETSEKVVI
                     FAAPGDEVAQACAAHLKALYEPVTVFVQYPSVVHNPSVVDKRSVAHDGNEYAGLLAQW
                     NPTRDGMNRFVFFDSYLESLSPNESADGPGYWFAAPMLLAQALGKRALNGGGGWPSVS
                     LTVVSKGMWSLDGEEACFPRKALARGLGIVLSQEYPGLRFRSIDLPNTGYEAFATGTK
                     HRTLIEEIRCDAPPAHVALRGRSRWERVFAPVKNPQGGREALKQNGVYLITGGLGGIG
                     LEIAEYLARHQQARLVLVARHVPDAIEDRLQRIRELGGEIHVVRADVTSAAAMSRAKS
                     DAESRFGAIDGIIHAAGSMPGSIVQRMSEESAWEVMKAKVQGARVLTEVFGDRPRDFI
                     LFFSSLRALAGGAGNADYAAANAYLDAVALDAWTRGRTDIKSVNWDGWAGVGMSLKAK
                     KPPGQEADPAPLSVEQGLRALTLALGIENPQVIVSSTDVGNLVTAMATSASVPGLPAL
                     KGDESGPSEVRPRPANLEQPYVAPRSDTEALLAGIWEQALGVSPIGVRDSFLALGGDS
                     VTSLQVAAKATGAGIRIGPFDTLERDSIAEVCEHLQTQPAEVKDDAGAEVTGDVPLTP
                     IQHWFF"
BASE COUNT          699 a         1205 c         1386 g          694 t
ORIGIN      
        1 ggagtttccc cccgtagcgc tcttcgaata tcccacgatt cgcagttgcg ccggctatct
       61 gcgtagtgtg cgcgccggta cgcacggcgc tgcggagtct gctcccggcg agagcaatga
      121 cggatccgag gagcgcgagc atctgctgct catggccgag accctccggg cagcaagcga
      181 tgcgcactga ggcagccgac aggaacagcg gactcgcgat cgcgatcgtc gggatgacgg
      241 cgcgtttacc gggggcacgt gatctggagg agttctggaa gctccttgcc gatggcaacg
      301 agggcatccg aagactgtcg gacgctgagc tgcgcgcggc gggactgagt gaggagcgct
      361 tgcgcgaccc ggatctgatc cgtggactgg gactgatcga tgagggcgag ctgttcgatg
      421 cgggcttctt cggttactcc agcagcgatg ccgagatact cgacccgcag cagcgtatct
      481 ttctcgaaca gtgctggatg gcgctggaga gcgcgggtta cagccctgag aagttcaatg
      541 gcgcgatcgg cctgtttgcc ggatgcggtc tcagtgccta caccacccgg ctggtggcca
      601 gcggctgggt ggcgcgttcg ggaataggct tccagaaaat cgtcggcaac gacaaagacc
      661 acctgaccac cgctgtcgcc tacaagctcg gtctgaccgg tccagcggtg acggttcaga
      721 ccacctgctc gacatcgttg gtggccgtgc acatggcttg ccgcagcctg ttgggcaggg
      781 agtgcgacgt ggcgctggcc ggtggcgtta ccgccggcct ggagttcgga tccgcgccta
      841 cctatcagga gggcaacatc ctctcccgcg atggtcactg ccgaccgttc gacgcacagg
      901 cctcgggaac tgtggtgagc aacggcgcag cggtcgtcgt gctgcgcagg ctggaggatg
      961 cactcaggga cggagataac attcgcgccg tcatccgggc gactgccgtg aacaacgacg
     1021 ggtcgctgaa ggtgggatac atgtcgccgt cggtggccgg ccaattcaag gtcgtgcgcc
     1081 gggccttgcg tctcgccgac gttcatccgg agacggtcga ctacgtagag gcccatggca
     1141 ccggaacact gaaaggagat cccatcgaag tgcgcgctct gacgcgcgcc taccgggagc
     1201 agaccgccag gtccgggtac tgtgccctgg gatcggtgaa ggccaacatc ggccatcccg
     1261 atacggccgc cggggccgcg ggtctgatca agacggtgct cgcgatgcag cacggagtca
     1321 ttccgccgac gccgcacttc acaacgccca accctgagtt ggagctcgaa aacagtccct
     1381 tcttcgtcaa caacaccgcc atcccgtggc cccgcgccgc tcggccacgt cgcgccgccg
     1441 taagctcgtt tggattgggt ggtacgaatg cgcatctgat cctcgagcag gcgcccgagg
     1501 tgccgcacgc tgcgccggat gacgaaccgc aactcctgct catttccgga aagacgccga
     1561 cagcgctggc ggcttcctgc agggggctgg ccggctacct gcggaaaaac tcgcagctcg
     1621 ctctgcgcga tgttgctttc acgttgcgct acggacgcgc ggatctgaag gagcgcgcct
     1681 ggttcgtggg ctcgcgtgtg cagattctgg agcagctgga ggcaggctcg gactgcgccg
     1741 ctggctccgc ctcgcgatgg ctgcaacagg cacgcgacta ctatcggtcc agcgcggcct
     1801 ttcgcgagct cgttgcgcag aacgccaagc gctatgccgc cggacagatc gagatcgaga
     1861 agctgctgca atccgcgacg caggacggag cagggttgcg cgatttggca gtgtccgtcg
     1921 gcgatccgga tgccggtcag gcattgcagc tcgcgctcag tgtcatgggt gagctgtgga
     1981 gacgaggtca ggtgcaggcg gtggctgcgc cgccggggat ggcggaaggg caacgcatcg
     2041 agctgcctca ttatcccttc gagcgcagtc gttattggat cgatacggcc gagagtccgc
     2101 gcccgtcccg ggattcggcg ccggtcgcgt cggatgtgag cggctggcta tacatcccgg
     2161 tatggcgcca atgcgcggcc gggccatcga gcggccggcc ggaaacgtca gagaaggtcg
     2221 ttatctttgc cgcgcccggt gatgaggtcg cgcaggcgtg tgcggcgcat ctgaaggctc
     2281 tctatgagcc cgtgaccgtc ttcgtgcaat acccgagtgt cgtgcacaac ccgagtgtcg
     2341 tggacaaacg gagcgtcgcg cacgatggaa acgagtacgc aggactgctg gcgcagtgga
     2401 atccgacacg agacggtatg aatcgcttcg tgttcttcga tagctatctc gaaagtctct
     2461 ccccgaatga atctgccgac ggccctggat actggttcgc ggcgccgatg ctgctggccc
     2521 aggcactcgg gaagcgagct ctgaacggcg gcggtggctg gccctcggtc agcctgaccg
     2581 tcgtgtcgaa gggcatgtgg agtcttgacg gcgaggaagc ctgtttcccg agaaaggcgc
     2641 tggcgcgggg cctgggaatc gtcctgtcgc aggagtaccc cggcctgcgc ttcaggagca
     2701 tcgaccttcc gaacaccggc tatgaggcgt tcgcgacggg aaccaaacat cggacgctga
     2761 tcgaggagat ccggtgcgat gcgccgcctg cgcacgtggc gctgcgtggc cgatcccgat
     2821 gggagcgggt gttcgcgccg gtgaaaaacc cgcaaggggg ccgcgaggct ctgaagcaga
     2881 acggggtgta tctgatcacc gggggcctcg gtggcatcgg attggagatc gcggagtatc
     2941 tcgcccggca tcagcaggca agactggtgc tggtcgcgcg ccacgtcccg gacgcgattg
     3001 aagaccgcct acagcggatt cgcgagctgg gtggagagat ccacgtggtg cgagccgacg
     3061 tgacgagcgc tgcagccatg tcccgtgcga aaagcgacgc cgagagccgg ttcggcgcga
     3121 tcgacgggat catccacgcg gccgggtcca tgcccgggag catcgttcag cgcatgtccg
     3181 aagagtccgc ctgggaagtc atgaaggcga aggttcaggg cgcacgtgtg ctcacggaag
     3241 tgttcggcga ccggccgcgc gatttcattc tcttcttctc ttcgctacgc gcgttggctg
     3301 gaggtgctgg taacgccgac tatgccgcgg ccaacgcgta cctggacgcc gtcgctctcg
     3361 atgcgtggac acgaggccgc actgacatca agtcggtgaa ctgggacggt tgggccggcg
     3421 tgggcatgag tctgaaggcg aaaaaaccac cggggcaaga ggccgatccg gctccgctga
     3481 gtgtcgagca gggcttgcgc gcgttgacgt tggcactggg catcgagaat ccccaggtga
     3541 tcgtttcctc taccgacgtc ggcaacctgg tgaccgcgat ggccacttcc gccagcgtgc
     3601 ctggactgcc cgcgctcaag ggcgatgagt caggcccgag cgaagtgcgt ccgcgcccgg
     3661 cgaatctcga gcagccgtac gtcgcgcccc gtagcgatac ggaggcactg ctggcgggga
     3721 tctgggaaca ggcgctgggg gtcagcccca tcggggtgcg ggacagcttc ctcgcgttag
     3781 ggggagactc cgtgaccagc ctgcaggtcg cggccaaggc gaccggagcc gggatccgga
     3841 tcggaccctt cgatacgctc gagcgcgatt cgatcgctga agtctgtgag catctgcaga
     3901 cgcagcccgc tgaggtgaag gacgatgcag gggccgaggt gaccggcgat gtacccctga
     3961 ccccgattca acattggttt ttcg
//
LOCUS       DASRHN010000044        13053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1169107, whole genome shotgun sequence.
ACCESSION   DASRHN010000044 DASRHN010000000
VERSION     DASRHN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169107"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..796
                     /locus_tag="VFB37_01640"
     CDS             122..796
                     /locus_tag="VFB37_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HZO21175.1"
                     /translation="MCRRLVIPGREEAEQEISVSNPWWSFSTRFNVAPAQGVPTARMH
                     EKDSEGVVMRWGLLPASRKREAPGDPHYTIASAAIEASEEFRPVWLYGQRCIVPVAGF
                     YLWQRSGSAHRQPFYVRVVNRPVFGVAALWDRFVTEDDDVIEGCALVTVSPNSLLAGI
                     AGTGGMPVILPREAYRLWLGGRPAEARSLLEAYPAERMVTHAVGPHVNFPEFDDPSLI
                     RPIRHG"
     gene            complement(797..1771)
                     /locus_tag="VFB37_01645"
     CDS             complement(797..1771)
                     /locus_tag="VFB37_01645"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZO21176.1"
                     /translation="MTALGSWAFPERLRPRQELLQFDLDKALASVVLVHTEVADTAFT
                     ATVLGTERLGHGVVIGSDGLILTIGYLIAEAESIWLTTNSGAVLAGHALGYDPVTGFG
                     LIQPLGALDAPPMPRGRSSAAAVGDSMTVIGHGGLGHALEARLIAKREFAGYWEYLLD
                     EALFTAPAHPQWGGTALVDRHGCLLGVGSLLVQEASGARTLDANMFVPVDLLEPILTD
                     MLRFGRPSRVPRPWLGMYATSLEGVLVVGGLTPGGPAQRAGVRLGDVLLEVAREPVSD
                     LAGMLRKLWSVGPAGSEIPVTVARAGNTSHLSVTSADRNDYLTQPRKH"
     gene            complement(1835..2482)
                     /locus_tag="VFB37_01650"
     CDS             complement(1835..2482)
                     /locus_tag="VFB37_01650"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZO21177.1"
                     /translation="MQEFRMGNSHAERIVSSNHATTSRAPRGDALLFARNFFRHPRML
                     GSIIPSSRFLIKQLLQPIDWEKARVIVEYGPGVGGITAEILRRMRPDACLIAIETNPD
                     FVGFLRKTLVDERFHIAQASAADVRQILQQHGHSKANYIISGIPLGAMPQRLRQPILQ
                     NTCEALEPGGSFLVYQFTTRVLPDLRRIFRYVKRKFEPLNVLPAHLFFCQQPGTA"
     gene            complement(2602..2925)
                     /locus_tag="VFB37_01655"
     CDS             complement(2602..2925)
                     /locus_tag="VFB37_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="involved in start site selection during the
                     initiation of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HZO21178.1"
                     /translation="MKDRPTFRGVVLRNATRSPPGQPARVPARGPVRVGREVAGRNGK
                     GVSVITGLPLSASELDRLAADLKKLCGAGGGVRAGVIEIQGDHRDRLLTELRKLGYEA
                     RRSGG"
     gene            complement(3007..3840)
                     /locus_tag="VFB37_01660"
     CDS             complement(3007..3840)
                     /locus_tag="VFB37_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO21179.1"
                     /translation="MKTVSLPGGDAVPVFGMGTWRMAENPARRAEEIATLRLGLNRGA
                     TLIDTAEMYGEGRAEQLVGEAIVGHRDEVFLVSKVYPHNATRTGVPRACERSLERLGT
                     DRIDLYLLHWRGSIPLQETMDAFAALQRSGKIRHYGVSNFDLSDMEELSASSSGRAAQ
                     TDQLLYNLTRRGLEWDLLPWLRERHIPLMAYSPVEEGRLLRDTRLQKFARECERTPAQ
                     VALAWLCAQREVLVIPKTGSRERLLENLACLERPLDRAELSELDRLFPPPSAATPLEM
                     I"
     gene            complement(3875..4414)
                     /locus_tag="VFB37_01665"
     CDS             complement(3875..4414)
                     /locus_tag="VFB37_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZO21180.1"
                     /translation="MTGTVRPLIALTALLGTLLSGLTTGAEGVVQEAQSLTQAKVSLP
                     DAIRLAEQSARGRAVSAAYELGSSSPPYYEIKVLHEAGRKLTRYDLNPNTGKVKGASD
                     ERIEPLFTHLRPSSVASARVSLSRAVETAEERAGGKATAAAIERGTEPLRYSIAIARL
                     DGTTENVQVNGSDGTVAAD"
     gene            4721..5398
                     /locus_tag="VFB37_01670"
     CDS             4721..5398
                     /locus_tag="VFB37_01670"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZO21181.1"
                     /translation="MNTQRGLSILAGLCIAGAAYAQSPPPPEQPSSQSNVTTPQGQPT
                     PDQPPGSTNPSSRQPDQANPATYPAPDSSGNSNPSSASSPHQRDVTSASNSEAQPTTA
                     SPDPRAASTPHQRDTMRMAAAGSISSGMDVQSREGKQVGTVVNVVNDSSGKPGYVVIA
                     DQSGRDTAVPYSAAKHMVHGNKIVVSDKRLASAPKVPESQLQDTSNTEWQGQADSYWS
                     KHGKKRS"
     gene            5705..7012
                     /locus_tag="VFB37_01675"
     CDS             5705..7012
                     /locus_tag="VFB37_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZO21182.1"
                     /translation="MAQRSRLHVPHPPSRPGDKPDFSYVRLSPAGAVPRPDVTATLPE
                     VEGLATALVRVLDDAHRAVGAWNPHLEPADLQVGLRHMLLTRVFDDRMQRMQRQGRIS
                     FYMKSTGEEAVSVAQGMALLPGDMLFPSYRNQGLYVVRGRSLTDLMCQCLSNTHDMCK
                     GRQMPVMYHWASGNIFSISGNLATQFPQAVGWAMAAAIRGEDRIAASWIGEGSTAEAD
                     FHHALTFAAVYGAPVILNVVNNQWAISTFQGFAGGEHRTFAARGPGYGIPGIRVDGND
                     FLAVHAVTSWAAERARLSGGPTLIEHVTYRASAHSTSDDPSRYRPKDEWRAWPLGDPI
                     VRLKQHLIALGEWSEERHSALEKELEAQVTACWKEAVSYGTLTEGPTLDAATMFEDVF
                     KHMPPHLARQREQLKALQGLEPPKGSPAQKELERQTRSLAKEG"
     gene            7025..8032
                     /locus_tag="VFB37_01680"
     CDS             7025..8032
                     /locus_tag="VFB37_01680"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZO21183.1"
                     /translation="MNMVQALNSAMDIMLQRDPRVVILGEDVGYFGGVFRVTDGLQRK
                     YGEHRVLDTPIAEGGIVAAAIGMGVNGLKPVAEIQFADYIYPAFDQVVSELARLRYRS
                     AGEFSAAVTIRTPCGGGIRGGQTHSQSPEGIFTHVSGIKVVMPSNPYDAKGLLISSIE
                     DEDPVVFFEPKRIYNGPFDGDPHKPAIPWSSHPSGEVPEGHYTLPIGRAAVVRPGSAV
                     TVLAYGTMVHVAAAAVRLCEIDAEIVDIRTLVPLDVETICESVCKTGHCVVVHEATGF
                     SGYGAELVATVQEGCFWKLEAPIRRVTGWDTPYPHAFEWEYFPGPARIAQALKSVMEE
                     A"
     gene            8029..9345
                     /locus_tag="VFB37_01685"
     CDS             8029..9345
                     /locus_tag="VFB37_01685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZO21184.1"
                     /translation="MSRYVFKLPDLGEGTVEAEIVGWHVKPGDQVSEDDVMVEVMTEK
                     AAVELPAPISGRVISTTGAPGDRVPVGSELIVFETASGVQDEAPSGATTDRSSGDTPA
                     VAAAAAAAPAAPRESRSASPPSAGGGRSNGHAAVSTEERPIRVATSPAIRRKAREAGV
                     DLTRLSGSGPHGRILRQDFEAFMSGRSAPTVLRPVNPAKERHQDRAATEEIKVIGVRR
                     LIAQRMSESKRTIPHFAYVEELDVTELESLRRHLNTKPALRAPGLTYLPFIVSALVRV
                     LRDFPQCNALYDTERGVIVRHHAVHVGIATQTGDGLKVPVVRDAQRLSLWELAEQMRR
                     VTEAARTNKATREELTGSTITVTSLGKLGGIASTPIINAPEVAIIGVNRAIERPVVHG
                     GAIAIRRMMNLSSSFDHRFVDGFDAAAMIQALKERLEHPATIFIDP"
     gene            9675..10520
                     /locus_tag="VFB37_01690"
     CDS             9675..10520
                     /locus_tag="VFB37_01690"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HZO21185.1"
                     /translation="MNAAVQERAASVLARLQYLEPGSEKPRSLEFEPPPGVPHTTAVY
                     RERAVSIQDMRPIATYLSLDREGFRLLTAPSSVWDFRDENLIRARYYPEAVALIRRVA
                     GAARVVVFDHTIRRRVPGALDRARGIPRQPVPRVHNDYTSESGPQRVRDLLAEEADEL
                     LRRRFCVLNLWRPLKGPVEDSPLGLADARTVKPSDLIATDLIYPDRRGEIYYVRFNPA
                     HRWYYAPAMGTDEVLLFKCFDSARDGRARFVPHSAFTDPNTAAGAAPRESIELRTLVF
                     FDEPA"
     gene            complement(10538..12784)
                     /locus_tag="VFB37_01695"
     CDS             complement(10538..12784)
                     /locus_tag="VFB37_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZO21186.1"
                     /translation="MTAPAVTVSRRAVIQGTGALLLSFVIPATRSREVASARHADAAL
                     DLPGSLAKNPLLDAWIRIDADGSVTAFTGKAELGQGIRTALLQVIAEELDVPWSRLTL
                     VTADTERTPNEGYTAGSHSVQDSGTALRHAAAQVRGILLAEAARHLSVPEQQLHTEAG
                     AVLGPNGDRVSYGELVASISLHVQAQPSSQLKDPARYSVMNHPVPRVDIPGKVSGAVA
                     YVQDLRPPGMLHARVVRPPGYRAALLEVNVNEVESMPGVMKVVRNGSFLAVIAQREFQ
                     AIQAMWSLAREARWSAGAALPDGSDIRQLLLGLPTRDETILDTQPNPARPSNHGLLTL
                     EASYSRPYIAHASIGPSCALAQFTSGVLTVWSHTQGVYPDRQAIAEMLHLPSDKVRCI
                     HVEGSGCYGHNGADDAAADAALLAFAIPDRPIRVQWMREQEHAWEPYGPAMLAQVRGA
                     LDTSGRIASWDYGIWSNTHSMRPGPAGALLAAQHLEPAFPIPEPRPIPQPEGGGDRNA
                     IPLYSLPQARVVHHFLPDMPVRISALRSLGAHMNVFAIESFMDELAVAARADPVEFRL
                     RHLEDPRARDIVSAAADEFRWSAGSAPGQGRGFAFARYKNLAAYCAVAMQVEVSPETG
                     RIHVRRAIAAVDAGEVINPDGLRNQIEGAILQSLSWTLFEQVRFDRAHITSIDWASYP
                     ILRFGSVPESVSVRIVERPGTPFLGCGEAGQGPAAAALANAVADATGKRFRELPLSPA
                     RIKAALAS"
     gene            complement(12781..>13053)
                     /locus_tag="VFB37_01700"
     CDS             complement(12781..>13053)
                     /locus_tag="VFB37_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21187.1"
                     /translation="DAQIRAQLELHLCRCGTHMRILRAIHRAAELIQGGRAAISTPTA
                     ASGPADGGETGNPSSPGEGQTGDSHDPLRRERDLSRHPQARERPER"
BASE COUNT         2283 a         4252 c         4248 g         2270 t
ORIGIN      
        1 cgcggacgat gcgagcgtat tcgcccgcta acgcgctggg ggctggctgc cttgaccact
       61 cggcctgtct ccgccagcgg acagttcccg ccctgggcgg gtcggcctta cccttggcgt
      121 gatgtgcagg cgactcgtaa ttcccggccg ggaggaggcg gagcaggaaa tctccgtcag
      181 caatccgtgg tggagttttt cgacccgctt caatgtcgct ccggctcaag gcgttcccac
      241 cgcgcgcatg catgaaaaag actcggaagg cgtcgtgatg cgctgggggc tgctgcctgc
      301 ctccagaaaa cgcgaagcac ccggcgatcc gcactacacg atcgcaagtg ctgcgatcga
      361 ggcttcggag gaattccggc ccgtctggct gtacggacag cgatgtatcg tgcccgttgc
      421 cggattctac ctctggcagc gctctggcag cgcccaccgc cagccgttct acgttcgggt
      481 cgtgaaccga ccggtgtttg gggtcgctgc gctctgggac cggtttgtca ctgaagatga
      541 cgatgtgatc gagggctgcg cgctggtcac ggtatctccc aactcattgc ttgcaggaat
      601 agccggcacg gggggaatgc cggtcatcct gcctcgcgag gcgtatcggc tctggctggg
      661 ggggcgtccg gcggaggccc gatctctact ggaagcctac ccggccgagc gcatggtgac
      721 gcatgcggtc ggtccgcacg tcaactttcc ggagttcgat gatccgagtc tgatacgacc
      781 catccggcac ggctgatcag tgttttcggg gctgcgtgag ataatcgttg cgatcggcgg
      841 acgtgacgct gaggtgcgag gtgttgccag cgcgagccac cgtcacaggg atctccgatc
      901 cggcagggcc gacgctccac agcttgcgaa gcatccccgc aagatcgctc acaggctccc
      961 tggccacttc gagcagcaca tcaccgaggc gcacgcccgc ccgctgcgcg ggtccgcccg
     1021 gagtcaggcc gccgacgacg agaacgccct ccagtgaagt ggcgtacatc ccgagccacg
     1081 ggcgcgggac acgcgagggt cggccgaatc tgagcatgtc ggtcaggatc ggctcgagca
     1141 ggtcaacggg cacgaacatg ttggcatcca gcgtgcgtgc accgctggct tcctggacca
     1201 gcagtgatcc cacaccgagc aggcagccgt gtcgatccac gagggccgtg ccaccccact
     1261 ggggatgggc gggagcggtg aagagcgcct catcgagcag gtattcccag tagccggcga
     1321 actcccgctt tgcaatgagc cgcgcctcga gcgcgtgtcc caggccgccg tgcccgatca
     1381 ccgtcatgct gtccccgacc gccgcggccg aagaacgtcc cctgggcatc ggtggtgcat
     1441 cgagcgcgcc gagtggctga atgagtccga agccggtgac gggatcgtag ccgagtgcgt
     1501 gcccggcgag gacggctccg ctgttcgtcg tgagccagat cgattcggct tcggcgatca
     1561 ggtagccaat ggtgaggatg agtccgtccg aaccgatgac gactccatgt cccagacgct
     1621 cggttccaag aacggtagcc gtgaacgcgg tgtcggctac ctccgtatgc acgagtacga
     1681 cggaggccag cgctttatcc agatcgaatt gcaacagctc ctgccgcggt cgcaggcgct
     1741 ctggaaatgc ccagcttccg agcgcagtca tgccccgtcc gcggcggatc cgccgcctcg
     1801 aaccgcgccg tcgaccggca ttgcgtccgc gaggctaggc cgtgcccggc tgctgacaga
     1861 agaagagatg agccggtaga acattgagcg gctcgaattt gcgcttgaca tatctgaaga
     1921 ttcgtctcag atccggcagt acccgggtgg tgaactgata gacgagaaag gagccaccgg
     1981 gctcgagggc ctcgcacgtg ttctgcagga tgggctgacg cagcctctgg ggcattgcgc
     2041 ccagtggaat accggagatg atgtagttcg ccttggaatg accgtgctgc tgcaggatct
     2101 gcctgacgtc ggcggcggag gcctgcgcga tgtgaaatcg ctcgtccacc agagttttgc
     2161 gcaagaatcc cacgaaatcg ggattggttt cgatggcgat caggcacgca tccgggcgca
     2221 tgcggcggag gatctccgcc gtgattccgc ccaccccggg gccgtactcg acgatcaccc
     2281 gcgccttctc ccagtcgatc ggttgcagta gctgcttgat caggaagcga gagctcggaa
     2341 tgatcgagcc cagcatgcgc ggatggcgga agaagttgcg tgcgaagaga agcgcgtctc
     2401 cgcgtggagc ccggctagtt gtagcgtgat tgctcgacac gatgcgttcc gcgtgactgt
     2461 tgcccattcg aaactcctgc atgataagta agttccggga ccccgcgccc ctacagcggc
     2521 ccgtgtccag ggcggacgtc cagcgggtcc gcctcggcta atctatagca atattcggca
     2581 ccgcgtgcca tccggagcgc ctcaaccccc ggaacgccgc gcttcatagc cgagcttgcg
     2641 cagctcggtg aggagccggt cccgatggtc tccctggatc tcgatcacgc cggcccggac
     2701 gcctccaccc gcgccacaga gcttcttcag atcggctgcg agacggtcca gttcggaggc
     2761 tgaaaggggc aggccggtga tgaccgagac gcctttgccg ttgcgccccg cgacttcccg
     2821 cccgactcgg acgggcccgc gcgcgggtac tcgcgccggt tgccccgggg gactgcgagt
     2881 cgcgttgcgc agaaccacac cccggaaggt cggtcggtcc ttcatgagaa tcggaacacc
     2941 acggtcgaga actggaacac accgtcgccg cccaacgcca ccgcacggtg gcactttacc
     3001 gtggcatcag atcatttcca acggagtcgc tgcgctgggc ggtgggaaca aacgatccaa
     3061 ctccgagagc tctgcacggt ccagcggccg ctccaggcag gcgaggtttt ccaacagccg
     3121 ctcgcgactt cccgtcttgg gaatgacgag cacctcgcgc tgggcacaaa gccacgcaag
     3181 ggcgacctgc gccggcgttc tttcgcattc gcgcgcaaac ttctgcagcc gggtgtcgcg
     3241 cagcagccgg ccttcctcga ccggggagta ggccatcaac ggaatgtgcc gttcgcgcag
     3301 ccacggcagc agatcccact cgagtcccct gcgggtcaga ttgtagagga gctgatcggt
     3361 ctgagcggcg cgcccggaac tgctggcgct cagttcctcc atgtccgaca gatcgaaatt
     3421 gctcacgccg tagtggcgaa tcttgcctga gcgctgcagg gcagcgaagg catccatcgt
     3481 ctcctgcaac ggaatgcttc ctcgccagtg cagcaggtaa agatcgatcc ggtcggtgcc
     3541 cagccgctcg agactgcgct cgcacgcgcg gggcacgccc gtacgcgtcg cgttatgtgg
     3601 gtataccttg gagaccagaa agacctcgtc acgatgcccc acgatggcct cgccgacgag
     3661 ctgctccgcc cggccctcac catacatctc tgccgtgtcg atgagcgtgg cgccgcgatt
     3721 caatccgagc cgcagcgtcg cgatctcctc ggcacgccga gccggatttt ccgccatccg
     3781 ccaggtgccc atcccgaaca ccggaaccgc atcgccacca ggaagggaga cagttttcat
     3841 gcggccattg ctcctgtttg cgcgggcgtt cggctcagtc tgccgcgaca gtgccgtcgg
     3901 aaccattgac ctgcacgttc tccgtcgttc cgtcgaggcg cgcaatcgcg atcgagtagc
     3961 gcaacggctc tgtgccgcgc tcgatcgcgg cggccgtcgc cttgcccccg gcgcgctctt
     4021 ccgcggtctc gacggcacgt gagagggaga cccgggcgct tgcgacggag gagggcctga
     4081 gatgcgtgaa gagcggctcg atgcgctcat cactcgcgcc cttcacctta ccggtgttgg
     4141 ggttgaggtc gtagcgcgtg agtttccggc cagcctcgtg cagcacctta atttcgtagt
     4201 agggtgggct gctgctcccg agctcgtacg ctgcactgac cgcgcgacct cgcgcgcttt
     4261 gctcggctaa ccggattgcg tccggcagcg aaaccttcgc ttgcgtcagg gactgtgctt
     4321 cctgcacgac gccttcggcg cccgtcgtca gccctgaaag cagggtcccg agaagggctg
     4381 tcagggcgat gaggggtcgg actgtgcctg tcatgttccg cgctccaagg tgcccgctgc
     4441 tgccaccctc ccgagctgct cggctgatcc gcaaccgtgg gctcgcccgc ggatccgcat
     4501 ggcccggggg aagcctcggg agtccctcga tggctcgcaa attgtgagcc cgaagcggcc
     4561 gccgcatgtt ggccgcgcct gcggccgagg ggaagctggg tccgggcccg cgccgtgtac
     4621 cccgaaccct cgcatgcagg tatggcgttt gcgcctcttg tgacaggacc gctctcatgc
     4681 cgtccccgtt aacaatccga tggcaagcga aggtgacgct atgaacactc aaagaggcct
     4741 cagcattctg gccggtctgt gtatcgcggg ggccgcttac gcgcagtctc cgcctccgcc
     4801 cgagcagccc agctcccagt ccaacgtgac cacgcctcag gggcagccca cgccggacca
     4861 accgccgggc tcgaccaatc cgtcctcgag gcagccggat caggccaatc cggccactta
     4921 tcccgccccg gacagcagtg gcaactcgaa tcccagctcg gcctcgagcc cgcatcagcg
     4981 cgatgtgacc tccgcgtcca acagtgaggc tcagccgacc accgcgagtc ccgacccgcg
     5041 ggccgcttcc acaccgcatc agcgggacac catgcgcatg gccgccgctg gcagtatttc
     5101 atcaggtatg gacgtgcagt cgcgcgaggg taagcaggtc gggaccgtcg tgaatgtcgt
     5161 caacgactcc tccggcaaac ccggttacgt agtgattgcc gaccagagtg gacgcgacac
     5221 ggcagtgccg tacagcgctg ccaagcacat ggtccacggc aacaagatcg tggtgagcga
     5281 caagcggctg gcgagcgcgc ccaaagtgcc cgagagccag cttcaggaca cctcgaatac
     5341 cgagtggcaa ggccaggcgg acagctactg gagcaagcac ggcaagaagc gcagctgagg
     5401 ctattgcctg atatgccggc tgcgcgccgg atctgaccag gccctctggc ctgcgctgat
     5461 cgtccagtgg ggcggcctct gaggccgccc cttccagctg gcggatgact gctggcctcc
     5521 attcagcacg gttgcgcctt ccgatcgcca tgctccccgc gagggaggcg ggcgtcacac
     5581 tcccagtgca tcgcccgttg cgcgaattct gcgttcgcgg tgtaaattgg aacgcctgtt
     5641 gcgctgcgcg caagtaatat tccattccac ccgtattccc tcggggcgct tcgagtaacc
     5701 gcacatggca caacggtccc gcctgcacgt gccccacccg ccgtcccgtc ccggcgacaa
     5761 gcctgatttc agttatgtcc ggctatcgcc tgccggcgca gtgccgcggc ccgacgtgac
     5821 ggcaacgctg cccgaagtcg agggactcgc caccgcgctc gtgcgcgtgc tggacgatgc
     5881 gcaccgggca gtgggtgcgt ggaacccgca tctcgagccg gccgatctgc aggtgggtct
     5941 gcggcacatg ctcctgacgc gcgtgttcga cgatcgcatg cagcgcatgc agcgccaggg
     6001 ccgcatttcc ttttacatga agtccaccgg ggaggaggcc gtctccgtgg cacagggcat
     6061 ggctctgctg cctggcgaca tgctgttccc ttcctaccgg aaccaggggc tgtacgtcgt
     6121 gcgcgggcgt agcctgaccg atctgatgtg ccagtgcctc tccaacactc atgacatgtg
     6181 caagggccgg cagatgccgg tcatgtatca ctgggccagc ggcaacatct tctcgatctc
     6241 ggggaatctc gccactcagt ttcctcaggc cgttggctgg gccatggcgg ctgccatcag
     6301 gggcgaggac cgcattgcgg ccagctggat cggggagggg tccaccgctg aagcggattt
     6361 ccaccatgcc ctgacgtttg ctgccgtata tggcgcgccc gtcattctga acgtggtcaa
     6421 taatcagtgg gccatctcga ccttccaagg cttcgccggc ggtgagcacc ggacattcgc
     6481 cgcgcgcggc cccgggtacg gcatcccggg catccgcgtc gatggcaacg attttcttgc
     6541 ggtgcacgcg gtgacgagct gggcggcaga gcgggcgagg ctcagtggcg ggccaactct
     6601 catcgagcac gtcacctatc gcgcctcggc acattcgacc agcgatgatc catcacgcta
     6661 tcggcccaag gatgaatggc gcgcctggcc gctcggagat ccgatcgtga ggctgaagca
     6721 gcatctcatc gcgctcggtg aatggtcgga agagcgccat agcgcgctcg aaaaggaact
     6781 cgaagcccag gtgactgcgt gctggaaaga agcggtgtca tacggaacgc tcacggaggg
     6841 tccgactctc gacgccgcca cgatgttcga agacgtgttc aagcacatgc caccgcacct
     6901 tgcgcgccag cgtgagcagc tcaaagcatt acaggggttg gagccaccca agggctcgcc
     6961 agcgcagaag gagctcgaac ggcagacccg ctccctggca aaggagggct gagctgtggc
     7021 gcacatgaac atggtccagg cgctcaattc cgccatggac atcatgctcc agcgagatcc
     7081 gcgtgtagtg atcctgggtg aggatgtcgg ctacttcggc ggtgtgttcc gggtgaccga
     7141 cggcctgcag agaaaatacg gcgagcatcg agtgctcgat acgccgattg ccgagggtgg
     7201 catcgtcgcc gcagcgatcg gcatgggtgt gaatggcctg aagcccgtgg cggagatcca
     7261 gttcgccgac tacatctatc cggctttcga tcaggtcgtg agcgagcttg cacggttgcg
     7321 ctaccgcagc gctggtgagt tttccgcagc ggtgacgatc cgcactccct gcggtggagg
     7381 gatacgtggc gggcagacac actcccagag cccggaaggg atcttcactc acgtaagcgg
     7441 catcaaggtg gtgatgccat ccaacccgta cgatgccaag ggcctgttga tcagctccat
     7501 cgaggatgag gatccggtcg tattcttcga gcccaagcga atctacaacg gtccgttcga
     7561 tggcgatccg cacaagcccg ccatcccctg gagctcccat ccgagcggcg aagttcccga
     7621 aggtcattac acgctgccca tcggtcgggc cgcagtcgtg cggcccggca gcgccgtgac
     7681 ggtgctggcc tacggaacca tggtgcacgt agccgccgcg gccgtccggc tttgcgaaat
     7741 cgacgccgag atcgtcgaca tccgcaccct cgtgcccctc gatgtggaga cgatctgcga
     7801 gtcggtgtgc aagaccgggc attgcgtggt cgttcacgaa gcgaccgggt tcagcggcta
     7861 cggtgcggag ctcgtggcca cagtccagga gggctgcttc tggaagctcg aagcgccgat
     7921 ccgacgggtt accggctggg acacgcccta tccgcatgcc ttcgaatggg aatactttcc
     7981 cggaccggcc cggatcgctc aggcgctgaa gagcgtgatg gaggaagcgt gagtcgctac
     8041 gtgttcaaac tgcccgacct gggcgaggga accgtggagg cggagatcgt cggctggcac
     8101 gtgaaaccgg gcgatcaggt atccgaggac gatgtcatgg tcgaggtcat gacggaaaag
     8161 gctgccgtcg agctgcctgc cccgatcagc ggccgcgtga tctccactac cggcgcaccc
     8221 ggtgatcggg tccccgtcgg gtccgagttg attgtcttcg agactgcctc aggcgtgcag
     8281 gatgaagctc cctcgggcgc gacaaccgac aggagctccg gcgatactcc cgctgtagcc
     8341 gcggccgcag ccgcggcacc ggccgcgcct cgcgagtccc gatctgcgag tccgcccagc
     8401 gcaggcggcg gccggtccaa cggacacgcg gcagtttcca cagaggagcg cccgatccgg
     8461 gtcgcgactt cgcctgctat ccggcgcaag gctcgcgagg cgggtgtcga tctgactcga
     8521 ctgagcggct ccggtccgca cggccgaatt ctgcggcagg atttcgaggc tttcatgtcg
     8581 ggacgctcgg cccccacagt gctgcgcccg gtgaatccgg ccaaggaacg ccaccaggat
     8641 cgggccgcca ccgaagagat caaggtcatc ggggtgcgcc gcctcatcgc acagcggatg
     8701 agcgagagca agcgcaccat tccgcatttc gcctacgtcg aggagctcga cgtgaccgag
     8761 ctcgagtcgc tgcggcgaca tctgaacacg aagcccgcct tgcgggcgcc cgggctcacc
     8821 tacctgcctt tcatcgtgag cgccctggtg cgcgtgctgc gggattttcc ccaatgcaac
     8881 gcgctttacg acacggaacg cggcgtgatc gtgcgacacc acgccgtgca tgttgggatc
     8941 gccacgcaga ccggtgacgg cctgaaggtg ccggtcgtgc gcgatgccca gaggctttcg
     9001 ctctgggagc tcgccgagca gatgcgccgg gtgaccgagg cggcgcgcac caacaaggcc
     9061 acgcgcgagg agctgacggg ctcgaccatc accgtgacga gcctgggcaa gctgggcggc
     9121 atcgccagca ctccgatcat caacgcaccc gaagtcgcca tcatcggcgt gaaccgtgcc
     9181 atcgagcggc cggtcgtgca cggcggcgcc atcgccatcc ggcgcatgat gaacctgtcg
     9241 tcttcgttcg atcaccgttt cgtggatggt ttcgatgctg ccgcgatgat tcaggcgctc
     9301 aaggagcgtc tggagcatcc ggccaccatc ttcatcgatc cgtgaagaaa aactaaacac
     9361 cacgcaacgc actctatttg ttcccgggca gggcccggag cacgctgtct cgcgtcacaa
     9421 ggcatccgca gctcgctgct tcagcctgga cttcccggag cggaggcgta catgagtgtt
     9481 ccacgattgt cgttgttcga aatcattctc gcacggctca gccttggcgc agcgccggtg
     9541 gatggcgcgt actggttcca gccccgcaag acgagcgggc atacccgaca gcactcgcag
     9601 cagaaaccag aggccgaccg gtcgcccgct tcgctgaact cgtcgcggcg tgcgccctca
     9661 aagagggccc actgatgaat gctgcagttc aggagagagc agcgagcgtg ctcgcgcgtc
     9721 tgcagtacct cgagccgggc agcgagaagc cgcgttcgtt ggaattcgag ccgccgccgg
     9781 gcgtgcccca tacgactgcg gtgtatcgtg agcgcgcggt gagcatccag gacatgcggc
     9841 cgatcgccac atacctgtcg ctcgaccggg aaggattccg cctgctgaca gccccgagca
     9901 gtgtgtggga cttccgcgac gagaatctga ttcgcgcgcg gtactacccc gaagcggtcg
     9961 cgctcatccg gcgagtggcc ggagcggctc gcgtggtggt attcgatcac acgatccgcc
    10021 gccgtgttcc gggtgccctc gacagggcga gaggaatacc gcgccagcct gtaccacgcg
    10081 tgcacaacga ctacacgagc gaatcgggcc cgcagagagt gcgcgatctg ctcgcagagg
    10141 aagccgacga gctgctgcgg cgccggttct gcgtcctcaa tctctggcga ccgctcaagg
    10201 ggcctgttga ggattctccg ctcggcctcg ccgatgcacg gaccgtgaag ccgagcgatt
    10261 tgatcgcaac ggatctgatc tatccggatc gcaggggcga gatctactac gtgcgattca
    10321 atccggcaca ccgctggtac tatgccccgg cgatgggcac cgacgaggtg ctgctgttca
    10381 agtgttttga ttcggcccgg gacggacgcg cccggttcgt gccgcactct gccttcaccg
    10441 accccaacac tgctgcaggt gctgcgcctc gcgagagtat cgagctgcgt acgctggttt
    10501 tctttgacga acccgcgtaa cacggcgcgc cgccccgtca gcttgccagc gctgccttga
    10561 tgcgcgccgg actcagagga agctcgcgga accgtttgcc ggttgcatcg gccacggcat
    10621 tggcgagtgc ggctgctgcc ggtccctggc cggcttcacc acagccgagg aagggtgtgc
    10681 cgggacgttc cacaatccgg acgctcacgc tctcgggaac cgatccgaag cgcagaatgg
    10741 gatagctcgc ccagtcgatc gaggtgatat gcgcgcgatc gaaccgcacc tgttcaaata
    10801 gcgtccagct cagggactgc aggatcgccc cctcgatctg attgcgcaga ccgtcggggt
    10861 tgatgacctc gccggcatcg actgccgcaa tggcgcgccg aacatggatg cggccggtct
    10921 cggggctcac ttccacctgc atcgcaacgg cgcagtacgc ggcgagattc ttgtaacgcg
    10981 caaacgcgaa ccctcgtccc tgcccgggag cggagcctgc cgaccagcgg aactcatcgg
    11041 cagcagcgct gacgatgtcg cgagcacggg gatcctccag gtgacgaagg cggaattcca
    11101 ccggatcggc gcgtgccgca acggcaagct cgtccatgaa gctttcgatc gcaaaaacgt
    11161 tcatgtgtgc gcccagggag cgcagggccg aaatgcgcac cggcatatcg ggcaggaagt
    11221 gatgcacgac gcgcgcttgc ggcagcgagt agagcggaat cgcgttgcgg tcgcctcccc
    11281 cttccggctg cggaatcggg cgtggctccg ggattggaaa ggccggctcc aggtgctggg
    11341 cggcgagcag tgcgcccgcg gggcctggcc tcatggagtg cgtattgctc cagatgccgt
    11401 aatcccagct tgcgatgcgt ccggatgtat cgagggcgcc acggacctga gcgagcatcg
    11461 ccggtccgta gggctcccag gcgtgctcct gctcgcgcat ccactgaacc cggatgggcc
    11521 gatccgggat tgcgaaggcg agcagggcag catcggcagc cgcatcatcg gcgccgttgt
    11581 gaccataaca gcccgatcct tccacgtgga tgcagcggac cttgtcggac ggcaggtgca
    11641 gcatctcggc gatcgcctgg cgatccgggt agacgccctg ggtatgtgac cagactgtca
    11701 ggactcccga tgtgaattgc gcgagggcac acgaaggtcc gatcgaggcg tgtgcgatgt
    11761 agggcctcga gtaggaagct tcgagggtaa ggaggccgtg gtttgatggg cgagccggat
    11821 taggctgcgt atcgaggatc gtttcgtccc gggtgggcaa acccagcagc agctgacgaa
    11881 tgtcagagcc gtccggcaac gcagcgcccg ctgaccagcg tgcctcacga gccaggctcc
    11941 acatggcctg tatggcctgg aattcgcgct gcgcaatgac tgcgagaaag ctgccgttgc
    12001 gcaccacctt catgactccc ggcatgctct cgacttcatt gacattcacc tccagcagcg
    12061 ctgcccggta gccgggtggc cgcacgaccc gcgcatgcaa catcccgggc ggccgcaggt
    12121 cctgcacgta ggcgacagcg ccgctgacct tgccagggat gtccactctg ggcaccggat
    12181 gattcatcac cgagtagcgg gccggatcct tgagttgcga gctcggttga gcctgcacgt
    12241 gcagcgagat cgatgccacg agctcgccat agctcacgcg atcgccattg ggtccgagca
    12301 cggcgccggc ttccgtatgc aactgttgct ccgggacact cagatggcgg gcggcctcgg
    12361 ccagcagaat accgcgcacc tgcgctgcgg cgtgccgcag cgccgtgccg ctgtcctgca
    12421 cggagtgact cccagccgtg taaccctcat tgggcgtgcg ttccgtatcg gctgtcacga
    12481 gagtcagccg actccagggc acatcgagct cttcggcgat gacctgcaac agcgcagtcc
    12541 ggattccctg accgagctcg gccttgcccg tgaatgcggt cacgctgccg tcggcgtcga
    12601 tccggatcca ggcatcgaga aggggatttt tcgccaggct tcccggaaga tcgagggctg
    12661 catcggcatg cctcgccgag gcgacctccc ggcttcgcgt agctgggatc acgaagctca
    12721 ggagcagagc gccggtgccc tgaatgaccg cgcggcgcga gactgtgaca gcgggcgcag
    12781 tcatcgctcc ggtcgctccc gtgcttgcgg atgcctcgac aggtctcgct cgcggcgtag
    12841 cgggtcgtgt gagtcacccg tctgcccttc acccggtgag ctcgggttgc cggtctctcc
    12901 gccgtcggca ggacctgaag ccgctgttgg tgtcgagatg gccgcgcgcc cgccctggat
    12961 gagctcggcg gcccggtgaa tggcacgcaa aattcgcatg tgcgtcccac aacgacatag
    13021 atggagctcg agttgcgcac ggatctgggc gtc
//
LOCUS       DASRHN010000045         7139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_509459, whole genome shotgun sequence.
ACCESSION   DASRHN010000045 DASRHN010000000
VERSION     DASRHN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509459"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="VFB37_01705"
     CDS             <1..1701
                     /locus_tag="VFB37_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZO21188.1"
                     /translation="AKDVAEGRERRQARKEAVRSEQKKAPVRAPPRIEPPMPIVQKSE
                     RVEKERQVPLFDPPKASELPPLTLLDDPPPREPLYSPEALEALSRLVEMKLKDFGIDA
                     EVVAVQPGPVVTRFELRPAPGVKASQITSLQKDLARALSVLSVRVVEVIPGKSVMGLE
                     IANEKRQVVTLGEIVRSRAYDDMSSPLALALGKDIGGQPMVADLARMPHLLIAGTTGS
                     GKSVGINAMVLSLLYKSTAEHVRLIMIDPKMLELSVYEGIPHLLAPVVTDMKQAANAL
                     RWSVAEMERRYQLMAALGVRNIAGFNRRVKEATDAGKPIRDPVMMLKAANDPTLDQSQ
                     IPDLTPLPYIVVVIDELADLMMIVGKKVEELIARLAQKARASGIHLILATQRPSVDVI
                     TGLIKANIPCRIAFQVSAKVDSRTILDQMGAETLLGHGDMLFLPSGTSLPTRVHGAFV
                     SDQEVHRVVEALKTPIPPNYIEEVLSGPRTPIPGIAPEEGEAAAALEDPEADPLYDEA
                     VKIVTTERKPSISYVQRRLKIGYNRAARLLEAMEIAGLVGPLQSNGAREVLVPAPPEA
                     "
     gene            1704..2351
                     /gene="lolA"
                     /locus_tag="VFB37_01710"
     CDS             1704..2351
                     /gene="lolA"
                     /locus_tag="VFB37_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HZO21189.1"
                     /translation="MGRKWPLMLLMLVSPLVAPLAWSDSPTPLDSYLQGMKTLHASFV
                     QTLVDAHGREIDRSTGTLIVERPGKFRWEIHPQGSGAAAGQLMVADGRNLWFLDRDLQ
                     QVTVKPMDAVLSATPAMLLSGTVDVRKNFTITTLGTREGLDWVRVEPHGSQADFRRAE
                     LGFASGELKRMVLDDKLGQRATVVFDRIERNAPVSPEEVSFTPPPGVDVIGTPQK"
     gene            2384..3700
                     /locus_tag="VFB37_01715"
     CDS             2384..3700
                     /locus_tag="VFB37_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZO21190.1"
                     /translation="MSPKDATRAEALRPLADRMRPRSLEEFVGQPHVLGPGKPLRQAI
                     ETGQLHSLILWGPPGTGKTTLARLIAQRADAQFIALSAVMAGVKDIRAAVESARAARA
                     ASGRPTLLFLDEVHRFNKAQQDTFLPFLEDGTLTFVGATTENPSFEVVSALLSRARVY
                     VLKALSVEDIVSLLQSALGDEERGLGKEHLVAEPQALELIARAADGDARRGLNMLELA
                     ASLAEAAQPHGLTESIAREVATGSRRRFDKGGDQFYDQISALHKAVRGTDPDGALYWL
                     CRMLDGGCDPLYIARRVVRMAIEDVGLADPRAFAMTLDAWEAYDRLGSPEGELAIANA
                     VVYLACVPKSNAVYVAMAEAMEDVEKFGTLEVPLRLRNAPTRLMKQLDYGRDYRYAHD
                     EPDAFAAGERYLPDELPERRYYRPVPRGLELKIAEALARLRGNKQS"
     gene            3697..5034
                     /gene="serS"
                     /locus_tag="VFB37_01720"
     CDS             3697..5034
                     /gene="serS"
                     /locus_tag="VFB37_01720"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZO21191.1"
                     /translation="MIDLKLLRSDPEAVARNLARRGFVLDVQALQAFEERRKSSQIEV
                     DRLRAERNANAKAVGIAKGKGEDTAALMGRSAGLTEELARAEESLSAVQATLEQWQAG
                     LPNLLHESVPDGNDEAANREIRRWGEPRSYDFKARDHVEIGERLGGLDFEAAARISGA
                     RFVVMRSQVARLHRALAQFMLDLHTREHGYTEVYVPYLVQPQALFGTGQLPKFEQDLF
                     AVRARGESTAAPAASPSQTAAGAPSYYLIPTAEVPVTNLVREQILTASELPLRFVCQT
                     PCFRSEAGAAGKDTRGMIRNHQFEKVELVQITRPTDSYAALEELTGHAETVLKKLEIP
                     YRTVALCAGDIGAGSAKTYDLEAWLPGQARYREISSCSNFEAFQARRMQARWRNPETG
                     KPEPVHTLNGSGLAVGRTLVAVLENYQNEDGTVSVPPVLQPYLPGLDRLIPAK"
     gene            5233..6537
                     /locus_tag="VFB37_01725"
     CDS             5233..6537
                     /locus_tag="VFB37_01725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZO21192.1"
                     /translation="MNAVENCVASDTERSRGARTPPAPDAEAVQDLAVPAGRSSVGGR
                     ALARLLAFLGNPPVEFILWTGERVVAAGAIEPVARVYIADRRTLFGLLADPQVRFGDG
                     YSEGRIRVEGDLVRLLETTYHAGAARTKKFSVVRRGVELIRRRHVNTLPGSRDNIHHH
                     YDIGNAFYSLWLGRTMAYTCAYYPTPQSTLDEAQVAKMDHVCRKLRLRAGETVVEAGC
                     GWGALALHMARQYGVKVRAFNISHEQIVFAREKALREGLGSQVEYVEDDYRNISGHYD
                     AFVSVGMLEHVGVDNYQTLGRVVQECLGGRGRGLIHSIGRNRPAAMHPWIEKRIFPGA
                     YPPALSEMLQIFEPWNLSILDIENLRLHYAHTLRDWLALYEGARDRVAAMFDERFVRM
                     WRLYLAGSIAAFTTGTLQLFQVVFAPGKSNDVPMTREHLYAR"
     gene            6567..>7139
                     /locus_tag="VFB37_01730"
     CDS             6567..>7139
                     /locus_tag="VFB37_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO21193.1"
                     /translation="MRTCDVVVVGAGPAGSAAARQLKLGGANVLVLDKARFPRLKLCA
                     GWITPEVVRDLGMDVAAYPHRFLTFERLHVHLKGLHFRLSSVQHSIRRFEFDAWLLER
                     SGAEVVQHTVRNIRAQAGEYIVDELYRCKYLIGAGGTRCPVYRSLFRELNPRATELQT
                     VTLEHEIEYPWTDPDCHLWFFERGLPGYSWY"
BASE COUNT         1225 a         2253 c         2425 g         1236 t
ORIGIN      
        1 gcgaaggacg tcgcggaggg tcgtgagaga cggcaagccc gcaaggaggc cgtgcgcagc
       61 gagcagaaga aggcgccggt gcgcgcaccg ccgcgtatcg agccgccgat gcccatcgtg
      121 cagaagagcg agcgggtgga gaaggaacgt caggtcccgc ttttcgaccc accgaaggcg
      181 agcgagttgc cgccgctcac gctgctggat gatccgccgc cgcgcgagcc gctgtattcg
      241 cccgaggcgc tcgaggcgct ctcgaggctc gtggaaatga agctcaagga tttcggcatc
      301 gacgcagagg tggtcgcggt gcagcccggt ccggtcgtga cgcgcttcga gctgcgaccg
      361 gccccgggcg tgaaagcgag ccagatcacg agcctgcaga aggatctcgc gcgcgcgctc
      421 tcggtgctga gcgtgcgcgt cgtcgaggtg attccgggca agagcgtcat gggactggag
      481 atcgcgaacg agaagcgcca ggtcgtcacg ctgggcgaga tcgtgcgctc ccgtgcctac
      541 gacgacatga gctcacccct cgcgctcgct ctgggcaagg acatcggcgg acagcccatg
      601 gtggccgatc tggcacgcat gccacacctg ctgatcgcgg gcaccacggg gtcgggcaag
      661 tcggtcggca tcaacgcgat ggtgctgtcg ctgctctaca aatccaccgc cgagcacgtg
      721 cggctcatca tgatcgatcc gaagatgctc gagctgtcgg tctacgaggg cattccgcat
      781 cttctcgccc ccgtagtcac ggacatgaag caggcggcca acgcgctgcg ctggtcggtt
      841 gcggagatgg agcggcgcta ccagctcatg gcagcgctcg gcgtgcgcaa cattgccggc
      901 ttcaaccggc gcgtgaaaga ggccaccgat gcgggcaagc cgatccgcga cccggtgatg
      961 atgctcaagg cggcgaacga tcccactctg gatcagtcac agattcccga cctgacaccg
     1021 ctgccctaca tcgtcgtcgt catcgacgag ctcgcggacc tcatgatgat cgtggggaag
     1081 aaggtcgagg agctgattgc acggctggcg cagaaggcgc gtgcctctgg catccatctg
     1141 attcttgcga cccagcggcc ttcggtcgat gtcatcacgg ggctgatcaa agccaacatt
     1201 ccctgccgca tcgcttttca ggtctcggcc aaggtggact cgcgcacgat cctcgatcag
     1261 atgggcgccg aaaccctgct gggccacggc gacatgctgt tccttccttc cggaacctcg
     1321 ctgccaacac gagtgcacgg agcgttcgtc tcggaccagg aggtgcatcg cgtggtggag
     1381 gcgctcaaga cacccattcc accgaactac atcgaggaag tcctcagcgg cccccgcacg
     1441 ccgattccgg gcatcgcgcc ggaggagggc gaggcggctg cggccctgga agaccctgag
     1501 gcggacccgc tctatgacga ggcggtcaag atcgtcacca cggagcgcaa gccctccatc
     1561 tcctatgtcc agcggcgcct gaagatcggc tacaaccgcg cggcgcggct tctggaagcc
     1621 atggagatcg ccggactcgt ggggccattg cagtcgaatg gcgcgcgtga agtgctggtg
     1681 ccggccccgc cggaggcatg agcatgggca ggaaatggcc gctgatgttg ctgatgctcg
     1741 tgtcgcccct cgtggcaccc ctggcctgga gtgacagccc cacgccgctc gacagttacc
     1801 tgcagggcat gaagacgctg cacgccagct tcgtacagac gctcgtcgat gcgcacgggc
     1861 gcgagatcga tcgttcgacc ggcacactga tcgtcgagcg accgggcaag ttccgctggg
     1921 aaatccaccc gcagggctcg ggggcggcgg caggtcagct gatggtcgcc gatggccgca
     1981 acctctggtt cctggatcgg gatctgcagc aggtgaccgt gaaaccgatg gatgcggtgc
     2041 tgtcggcgac gcccgcgatg ctgctgtctg gcaccgtgga tgtgcgcaag aacttcacca
     2101 tcaccacgct cggcacgcga gagggtctcg actgggtgcg cgtcgagcct cacggcagcc
     2161 aggcggactt tcggcgcgcg gagcttggat ttgcctcggg cgagctcaag cgcatggtgc
     2221 tcgatgacaa gctgggacag cgggcgaccg tcgtgttcga tcgcatcgag cgtaatgcgc
     2281 cggtgtcccc cgaggaggtc agcttcacgc ctccccccgg agtggatgtc atcgggacac
     2341 cgcagaaatg aggctggtgc cgcatcgccc ggcgtaatcg ctgatgagcc caaaggatgc
     2401 cacgcgcgcc gaggccctgc gcccgctcgc tgatcggatg cggccgcgct cgctcgagga
     2461 gtttgtcggc cagccgcatg tgctcgggcc cggcaagcca ctgcgccagg ccatcgaaac
     2521 cggacagctc cactcgctga tcctgtgggg ccctccggga accggcaaga cgacgctcgc
     2581 gcggctgatt gcccagcgcg cggatgcgca gttcatcgct ctttcggcag tcatggcggg
     2641 cgtcaaggac atccgtgccg cggtggagag cgcgcgggcg gcccgtgcgg cgagcgggcg
     2701 gccgacattg ttgtttctgg atgaggtgca tcgtttcaac aaggcgcagc aggatacgtt
     2761 tttgcccttc ctggaggacg gcacgctgac cttcgtgggc gccaccacgg agaatccgtc
     2821 cttcgaagtg gtgagcgcac tgctctcacg cgcgcgagtg tacgtactca aagcgctgtc
     2881 ggtggaggac atcgtttcgc tgctgcagtc ggcactcggc gatgaggaac gtggcctcgg
     2941 caaggagcat ctcgtggccg agccgcaagc cctcgagctc atcgcgcgcg ccgccgacgg
     3001 ggatgcgcgc cgcgggctga acatgctcga gctcgccgcc tcgctggctg aagcggctca
     3061 gccacacggg ctcaccgagt cgattgccag ggaggtggcc accggcagtc gccgtcgctt
     3121 cgacaagggc ggcgatcagt tttacgacca gatctcggcg ctgcacaagg cggtgcgggg
     3181 caccgatccg gatggggcgc tctattggct atgccggatg ctcgatgggg gttgcgatcc
     3241 gctctacatc gcgcgtcgtg tggttcgcat ggcgatcgag gatgtgggcc ttgccgatcc
     3301 gcgcgctttc gccatgacgc tcgatgcgtg ggaagcctac gatcgactgg gcagcccgga
     3361 aggcgagctg gcaattgcca atgcggtcgt gtatctggcg tgcgtgccca agagcaacgc
     3421 ggtgtatgtt gcaatggccg aggccatgga ggatgtggag aaattcggaa cgttggaggt
     3481 gcccttgcgc ctgcggaatg cgccgacacg cctgatgaag cagctggatt atgggcgcga
     3541 ttatcggtat gcgcacgacg agccggacgc ctttgccgca ggcgagcgat acttgcctga
     3601 cgagctgccc gaacggcgct attaccggcc ggtgccgcgg gggctggagt tgaagatcgc
     3661 tgaggcgctg gcgcgcctgc ggggaaacaa gcaatcgtga tcgacctcaa gttgctgcgt
     3721 tcggatccgg aagctgtggc gcgcaacctc gcgcggcgag ggttcgtgct ggacgtccag
     3781 gccctgcagg cattcgagga gcggcgcaag tcctcccaga tcgaagtcga tcgcttgcgc
     3841 gccgagcgca atgccaacgc gaaggccgtc ggcattgcca aagggaaggg ggaggacact
     3901 gcggcactca tgggacgcag tgcggggctg accgaagagc tcgcgcgcgc ggaggagagc
     3961 ttgagcgccg tgcaagccac gctcgagcaa tggcaggcgg gacttccgaa tctgctgcat
     4021 gagtccgtgc cggacgggaa tgatgaggcg gccaatcgcg agatccgccg ctggggcgag
     4081 ccgcgctcct atgacttcaa ggcacgcgat catgtggaga tcggcgaacg tctcggcggg
     4141 ctcgatttcg aggcggcagc gcgcatctcc ggcgcacgct tcgtggtcat gcgctcgcag
     4201 gtcgcccgac ttcaccgcgc cctcgcgcag ttcatgctcg acctgcatac ccgcgagcac
     4261 ggctacaccg aggtgtatgt gccctacctg gtgcagccac aggctttgtt cggcaccggc
     4321 cagctcccga aattcgagca ggatctgttc gccgtgcgcg ccaggggtga gtccaccgcg
     4381 gcgccggccg catccccctc acagaccgcc gccggagcgc ccagctacta tctgatcccg
     4441 accgccgagg taccggtgac caacctcgtg cgcgagcaga ttctgacagc cagcgagctg
     4501 ccgctgcgat tcgtctgcca gaccccttgt ttccgctcgg aagcgggagc cgcaggcaag
     4561 gacacccgcg gcatgatccg caatcaccag ttcgagaagg tcgagctggt gcagatcacc
     4621 cgccccacgg actcgtatgc cgcgctcgag gaactgaccg ggcatgccga gacggtgttg
     4681 aagaagctcg agattcccta ccggacggtt gcactctgcg ccggggacat cggagccggc
     4741 agtgccaaga cctacgatct ggaggcttgg ctgcctggcc aggcgcgcta tcgggaaatc
     4801 tcctcgtgca gcaatttcga agccttccag gcgcggcgga tgcaggcccg ctggcgcaat
     4861 cccgagacgg gcaagcctga gccggtgcat acgctcaacg gctcgggact ggcagttggc
     4921 cgcacgctgg tcgccgtgct ggagaactat cagaacgagg acggaacggt gtcggtcccg
     4981 cccgtgctgc agccgtacct gccgggcctg gacaggctga ttccagcgaa gtagctccgc
     5041 gcccgggcgg ctccgcagca ggggtgacgg ctggctctgg gggatctggg gagggactcc
     5101 gccgctctag gcgctggcgt ccatttgctc ggccggcccg gatggggcag attgccccct
     5161 tccgggcgcg aggagccgtt gcgccgggcc cattcgttgc taaattagcg ccaggatgga
     5221 cggagaataa gagtgaatgc tgtcgaaaac tgcgtagctt ccgacacgga acgctcccgg
     5281 ggagctcgaa cgccgcctgc gccggacgcc gaggccgtcc aggacctcgc cgttcccgct
     5341 gggcgttctt ccgtgggtgg gcgtgcgctc gcccggctgc tggccttcct cggcaatcca
     5401 ccggtggaat tcattctctg gaccggagag cgggtggtcg ccgccggggc gatcgagccc
     5461 gtggcccgcg tctacatcgc cgatcgacgc acactgttcg gtctgctggc ggatccccag
     5521 gtccgcttcg gggacggcta cagcgaaggg cgcatccggg tcgaaggcga tctggtccgg
     5581 ttgctcgaga ccacttacca cgctggcgcg gcacggacga agaaattctc cgtcgttcgc
     5641 cgcggcgtcg agttgatccg ccgcaggcac gtcaacacgc tgccgggatc gcgggacaac
     5701 attcaccatc actacgacat tggcaacgcc ttctattctc tgtggctggg ccgcacgatg
     5761 gcctacacct gcgcctacta tccaacaccg caatccacgc tggatgaggc gcaggtggcc
     5821 aagatggatc atgtctgccg caagctgcgg ctgcgagccg gtgaaaccgt cgtcgaagcc
     5881 ggctgtggct ggggcgcctt ggcgctgcac atggcgcgcc agtatggcgt gaaggtcaga
     5941 gcattcaaca tttctcacga gcagatcgta ttcgcgcgcg agaaggcgct ccgtgagggt
     6001 ctcggctcgc aggtcgaata cgtggaagat gactatcgca acatctccgg ccattacgac
     6061 gcattcgtct ccgtcggcat gctggagcac gtcggggtcg ataattacca gactcttgga
     6121 cgggtcgtgc aggagtgcct cggcggccgc gggcgtgggt tgatccactc gatcgggcgc
     6181 aaccgtccgg ctgcgatgca tccctggatc gagaagcgga tcttccccgg cgcctatcct
     6241 cccgcgctca gcgagatgct gcagatcttc gagccctgga acctgtcgat cctcgacatc
     6301 gagaacctcc ggctgcacta cgcgcacacc ctgagagact ggctcgctct ctacgagggc
     6361 gcgcgtgacc gcgtggcggc gatgtttgat gagagattcg tgcggatgtg gcggctgtac
     6421 ctggccggct cgatcgctgc gttcaccacc ggcacgctgc agcttttcca ggtggtcttt
     6481 gccccgggta agagcaatga cgtgcccatg acccgagagc atctttacgc gcggtaatcc
     6541 cgtcacccgg caacctcgaa gcttgcatgc gaacctgtga tgtcgtggtc gtcggcgcag
     6601 ggccggccgg ctcggccgcc gctcgccagt tgaagctcgg tggggcgaac gtgctggtgc
     6661 tcgacaaggc gcgcttccca cggctcaagc tgtgcgccgg ctggatcact cccgaagtgg
     6721 tacgcgacct cgggatggat gtggccgcct atccgcatcg ctttctcacc ttcgagcggc
     6781 tgcatgttca tctgaaggga ctgcatttcc ggctctcgag cgtgcagcat tccattcgtc
     6841 gtttcgagtt cgacgcctgg ctgctggagc gatcgggcgc cgaagtggtg cagcacaccg
     6901 tgcgcaacat ccgtgcacag gccggcgagt acatcgtgga tgagctgtac cgctgcaagt
     6961 atctgatcgg ggcgggcggg acgcgctgcc cggtctacag atccctgttt cgcgagctga
     7021 atccgcgtgc gacggagctg cagaccgtga ctctcgagca cgagatcgag tacccgtgga
     7081 cggatccgga ttgccacttg tggtttttcg agcggggctt gcccgggtat tcgtggtac
//
LOCUS       DASRHN010000046         6110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_344815, whole genome shotgun sequence.
ACCESSION   DASRHN010000046 DASRHN010000000
VERSION     DASRHN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344815"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2563
                     /locus_tag="VFB37_01735"
     CDS             <1..2563
                     /locus_tag="VFB37_01735"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZO21194.1"
                     /translation="RKEAYMLGAIITLLLVAAAAYAVTQVGGVITLVFIAAVLFLAYR
                     RVSLLVFTATFTVLLGAYTWFGASSAPAGLWKGFLWVLLAALWLLNLRPLRKALLTRP
                     FMRTYLKLLPRMSQTEREALEAGTVWWDGELFTGAPQWSRLLSANAPQLSAEEQAFLD
                     GPCEELCGMLDDWNITHELGDMPPQVWEFLKSRGFFAMIIPKKYGGLEFSAYAHSCVL
                     VKVASRSATASSTVAVPNSLGPAELLNHYGTQEQKDYYLPRLARGEEVPCFALTGPRA
                     GSDAASIPDTGVVCKGMWQGREIVGLRLNFSKRYITLAPVATVIGLAFRMFDPEQLLG
                     GKTDIGITCALIPRHTAGVTIGRRHFPINVPFQNGPIQGKDVFVPLDFIIGGTKMAGA
                     GWRMLVEQLSVGRCISLPSNATGGAKAGVWATGAYARIRRQFNMPVGRFEGVEAVIAR
                     MVGLTYTMDAARSVTAGAIDGGEKPSVPSAMLKYHVTEMGRQVANDAMDVHGGKGICL
                     GPRNYLARGYEVVPVAITVEGANLLTRSLIIFGQGAVRCHPFVLKEMTAARNPDRARG
                     VDDFDRALFGHIGFTISNAVRSFIMALTHARFTHVPVRGQTARYFQHIVRFSASFAFA
                     VDVAMLTLGGYLKKKENLSARLGDVLSCMYLASMVLKHHENEGRRAEDLPIVEWACRN
                     LLYHAQEQMHGFLRNFPNRFLAATMRLFVFPRGRTYSAPGDRLGRTVAGLVTQATETR
                     ERLCRYIFRTLQAGNPLGLLQEALLLAQSVEPIEKRIRVEGVKTGKVTALDLPGQIQQ
                     AVAAGIVSETEGAALREYDRKVLELISVDDFAQHELGTKAEPVPQTAALTSVHVA"
     gene            2582..3733
                     /gene="dinB"
                     /locus_tag="VFB37_01740"
     CDS             2582..3733
                     /gene="dinB"
                     /locus_tag="VFB37_01740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZO21195.1"
                     /translation="MNRAILHVDMDAFYASVEQRDDPSTAGKPVIVGGLGGRGVVAAA
                     SYEVRRYGVRSAMPMGTALRLCPHAICIPPRMARYKEVSEQVFAVFREITPLVQGLSL
                     DEAFLDVTASTVLFGDAVNIARRIKSRIRAITGLTASVGVASNKLVAKIASDLSKPDG
                     LSVVEEGRVREVLDPLSIRRLPGLGAKTGARVEALGISTMADLRTAPDALLRPLFGRY
                     TQRVRDRASGIDERAVHPDLEEKSISAEDTFDQDVREPPTLHAQLARLAELACVRLRK
                     RRLMAACIGIKIRRSDFSTFTRQRAVAPPTHEGRTIANVAGELLARWLAENPGAKLRL
                     LGVVLSDLSPASQLGLFEGVPHFGRLDAALDEARARFGNHALRRGHGIK"
     gene            3743..5449
                     /locus_tag="VFB37_01745"
     CDS             3743..5449
                     /locus_tag="VFB37_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO21196.1"
                     /translation="MYLSFFGLNEKPFAITPDPRYLYLSERHAEALAHLLYGINEAGG
                     FVQLTGEVGTGKTTVVRSLLAQTPKNAEIALILNPKMTATEFLLTICEELGIGVPDSA
                     TESLKDLVDILSGYLLRAHANGRRVVVVVDEAQNLAPAVLEQVRLLTNLETNTQKLLQ
                     IILIGQPELRELLARNELRQLAQRITGRYHLNPLTYEETAAYVRHRLRVAGATTELFT
                     PPALAEVFRLSAGVPRVINVVCDRALLGGYSTDRHRVTGSLVRDAAAEVFGRRFTPHW
                     LSWLGGAVAALLLVAAIALVWAFKPWSSRAPAGVAPAAVAKPAKLTSTPITRPAVVGA
                     STPASSGADPGKALQVSKASLTGRELTALLAQHAGETDTDSAFTKLFGLWRIRYVPAT
                     VDPCTQAASQGLECVNQRGSFGQLRLYNRPAILMLNDEAGASHQVVLTALDDERAAID
                     LGGGSREVSIGELSRFWFGDFVMLWRPGTSQVKVLSEGMRGDDVRWLRESLQKIEGEH
                     SDQPVSDVFDADLGRLVREFQRRHRLTVDGIAGVQTQIVLASAVAPADSPLLYLADTH
                     GS"
     gene            5453..>6110
                     /locus_tag="VFB37_01750"
     CDS             5453..>6110
                     /locus_tag="VFB37_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21197.1"
                     /translation="MSFILDALKKSESDRQRQTGPALFEVKIAPPKSRFPLWAVSIAV
                     LLAVNAAVGGWLLLRHSSSSPAQAPAAVPALAQSPAPVTSGAAPSAALTTEAITTAPA
                     GSGPPAAAPAQPSPATLAQPTRTYSAGAGTGRTDAVGAATSPAPIGAGGSGEPTLATN
                     GKPSELPQGSSQEAPTANPDDYAPAAEPKAGPGLGSHVKVGTSSGYILYQDAAVVPGA
                     T"
BASE COUNT         1016 a         2033 c         1994 g         1067 t
ORIGIN      
        1 acggaaggaa gcctacatgc tcggcgcgat catcacgctt ctcctcgtgg cagccgctgc
       61 ctatgccgtc acacaggtgg gcggcgtcat tacgctggtc ttcatcgccg cagtgctgtt
      121 tctggcttat cgccgggttt cgctgctggt gttcaccgcc acgttcaccg tgctgttggg
      181 ggcttacacc tggttcggcg catcgagcgc cccggccggc ctctggaagg ggttcctctg
      241 ggtgctcctc gccgcgttgt ggctgctcaa cctcagaccg ttgcgtaaag cgctgctcac
      301 gcggcctttc atgcggacct acctcaagtt gctgccacga atgtcgcaga cggagcgcga
      361 ggcactcgag gccgggacag tctggtggga tggcgagctg ttcaccggcg cgccgcaatg
      421 gtcccggctg ctctcggcca atgcgccgca gctttccgcg gaagagcagg cgtttctcga
      481 cgggccgtgc gaagagctct gcggcatgct cgatgactgg aacatcaccc acgagctcgg
      541 cgacatgccg ccgcaggtgt gggagttcct gaagtcgcgt ggcttcttcg ccatgatcat
      601 tccgaagaag tatggcggcc tggaattttc cgcttacgcc cattcgtgcg tgctggtgaa
      661 ggtcgcaagc cgcagcgcga ccgcctcctc gaccgtcgcc gttcccaact cgctcggccc
      721 cgccgagctg ctgaaccact acggcacgca ggagcagaag gactactacc tgccgcgact
      781 ggcacgcggc gaggaagtgc cctgcttcgc gcttaccggc ccgcgcgccg gctcggatgc
      841 ggcctcgatc cccgatacgg gtgtggtctg caagggcatg tggcagggac gcgagatcgt
      901 cggcctcagg ctgaacttct ccaagcgtta catcacgctt gctccggtag cgaccgtgat
      961 cggcctcgcc ttcaggatgt tcgaccccga gcaactgctc ggcgggaaaa ccgacatcgg
     1021 catcacctgt gcgctgattc cgcgccatac ggccggcgtg acgatcggac gcaggcactt
     1081 cccgatcaac gtgccgttcc agaacgggcc gattcagggc aaggacgtct tcgtgccgct
     1141 cgatttcatc atcggaggaa ccaagatggc aggcgcgggc tggcgcatgc tggtcgagca
     1201 actctcggtc ggacgttgca tctcgctacc ctccaacgcc accggcgggg ccaaggcggg
     1261 cgtctgggcc acgggcgcgt acgcgaggat tcgccggcaa ttcaacatgc ccgtgggcag
     1321 attcgaaggc gtcgaggcgg tgatcgcccg gatggtgggc ctgacctaca cgatggatgc
     1381 tgcgcgctcg gtgacggcag gggccatcga tggcggagag aagccttcgg tcccttccgc
     1441 gatgctcaag tatcacgtga cggaaatggg ccggcaggtg gccaatgacg ccatggacgt
     1501 ccatggcggc aagggtatct gtctgggtcc tcgaaactat ctcgcccgcg gctatgaggt
     1561 ggtgcctgtg gccatcaccg tggagggtgc aaacctcctc acgcgcagcc tgatcatctt
     1621 cggacagggc gccgtacgtt gccatccgtt cgtactcaag gagatgaccg ctgcacgcaa
     1681 tcccgaccgg gcgcggggag tggacgactt cgaccgggct ttgttcggcc acattggctt
     1741 cacgatttcc aacgccgtac gctcattcat catggcgctc acgcatgcgc gctttacgca
     1801 cgtgccagtg cgcgggcaga cagcgagata tttccagcac attgtccgct tcagcgcctc
     1861 gttcgccttc gccgtcgatg tggccatgct cactctcggc ggctatctga agaagaagga
     1921 gaatctgtcg gcgcgactcg gcgatgtgct ctcctgcatg tacctcgcct cgatggtgct
     1981 caagcatcat gagaacgagg gtcgccgcgc cgaggatctg ccgatcgtcg agtgggcctg
     2041 ccgcaatctg ctctatcacg cccaggagca gatgcacggc ttcctgcgca acttcccgaa
     2101 tcggtttctt gccgccacca tgcgcctgtt cgtctttccc cgcggccgta cgtactccgc
     2161 gcccggcgac cgtctgggcc gcaccgtcgc gggtctcgtg acccaggcga cggagacgcg
     2221 cgaacggctc tgccgctaca tcttccggac tctgcaagcc ggcaatccgc tgggtctgct
     2281 gcaggaagcg ctgctgctcg cgcagagcgt cgagcctatc gagaaacgca tccgggtcga
     2341 gggggtcaag accggcaagg tcacggcact cgatctgcct ggccagatcc aacaggcggt
     2401 cgccgcaggg atcgtctcgg aaaccgaggg ggccgctctg cgggagtacg accgcaaggt
     2461 gctcgagctg atcagcgtgg atgatttcgc ccagcatgag ctgggtacga aagccgagcc
     2521 cgttccccag accgcggccc tgacgagcgt gcatgtcgcc tgaatcaagc ggcactcgcc
     2581 catgaaccgc gcgatcctgc atgttgacat ggatgcgttc tacgcatcgg tcgagcagcg
     2641 ggacgatccc tccactgcag ggaagcccgt gatcgtcggt ggccttggcg gcaggggcgt
     2701 cgtcgcggcg gcgagctacg aggtccgcag gtatggggtc cgctctgcga tgcccatggg
     2761 cactgcgctg cgcttgtgcc cgcacgcgat ctgcatcccg ccccgcatgg cgcgctacaa
     2821 ggaagtatcc gagcaggtgt tcgccgtctt ccgcgaaatc accccgctcg tgcaggggct
     2881 gtcgttggac gaggccttcc tggacgtgac cgccagcacg gtactgttcg gtgacgcggt
     2941 caacatcgcc cggcgcatca agagccgcat ccgggcgatc accggattga cggcctcggt
     3001 cggggttgct tccaacaagc tggtcgccaa gatcgcttcc gatctgagca agcccgatgg
     3061 tctcagtgtg gtcgaggagg gccgggtgcg ggaagtcctg gatccgctct cgattcgccg
     3121 cctgcctgga ctcggagcca agacgggagc ccgggtcgag gcgctgggca tctcgacgat
     3181 ggcggatttg cgcactgctc cggatgccct cctccggccc ctgttcggac gctacacaca
     3241 acgagtccgc gaccgggcct cggggatcga cgagcgcgcg gtgcaccctg acctggagga
     3301 gaagtccatc agcgccgagg acactttcga ccaggacgtg cgggagccgc cgaccctgca
     3361 cgcacagctt gcgcggctgg cggagctggc ttgcgtccgg ttgcgcaaac gtcgcctcat
     3421 ggcagcctgc atcggcatca agatccgccg ctcggacttc tcgacgttca cccgccagcg
     3481 ggccgtggcg cctccgacac acgaggggcg tacgatcgcg aacgttgccg gggagttgct
     3541 cgcgcgctgg ctggcggaaa accccggcgc gaaacttcga ctgctggggg tggtcttgag
     3601 tgatctcagt cccgcctcgc agctcgggct gttcgagggt gtcccgcatt tcggacgcct
     3661 ggacgccgcg ctcgatgagg cccgggcccg gttcggaaat cacgcgctgc ggcgcggtca
     3721 cggcatcaag tagcatcggc tcatgtacct gtccttcttc ggcctgaacg agaagccgtt
     3781 cgcgatcacc cctgatccgc gctatctcta tctgagcgaa cgccacgccg aagctctcgc
     3841 tcatctgctg tacggtatca acgaggctgg cggattcgtc cagctcaccg gcgaggtcgg
     3901 aacgggcaag accacggtcg tgcgctctct gctcgcgcag accccgaaga acgccgaaat
     3961 cgcgctcatc ctcaatccga agatgaccgc taccgagttc ctgctcacga tctgcgaaga
     4021 gctgggtatc ggagtgccgg actcggccac cgaaagcctc aaggatctcg tggacatcct
     4081 cagcggctac ctgctgcgcg cgcacgcgaa tgggagacgc gtcgtggtgg tcgtggatga
     4141 ggcccagaat ctcgccccgg cggtgctgga gcaggtgcgc ctgctcacca acctcgaaac
     4201 caacacccag aagctgctgc agatcatact catcggccag cccgagctgc gcgagctgct
     4261 ggcgcgaaac gagctccgac agctcgcaca acgcatcacc ggacgctatc acctcaatcc
     4321 gctcacgtac gaggaaactg cggcctacgt caggcacagg ctacgcgtcg cgggtgcgac
     4381 gaccgagctg ttcactcctc cggccctggc ggaagtattc cggctttcag caggggtgcc
     4441 gcgggtcatc aacgtggtgt gcgatcgcgc cctgttgggc ggctactcga ccgatcggca
     4501 tcgcgtgacg ggcagtctgg tgcgagatgc ggccgcggaa gtgtttggca ggcgcttcac
     4561 gccacactgg ctgtcatggc tcggcggcgc cgttgccgcg ctgttgctcg tcgccgccat
     4621 cgcactcgta tgggcgttca agccctggag cagtagggct ccagcggggg tggcacccgc
     4681 cgcggtcgcc aagcccgcga aactgacctc caccccgatc acccggccag ccgtggtcgg
     4741 cgccagcacg ccggcctcct ccggagcaga ccctggcaaa gcgctgcagg tgagcaaagc
     4801 cagcctgacc gggcgcgagc tcaccgcgct gctcgctcag catgccggcg agactgacac
     4861 cgacagcgcg ttcaccaagc tcttcggcct gtggcggatc cgctatgtgc cggccaccgt
     4921 ggacccgtgc acgcaggcgg ccagtcaggg actggaatgt gtcaaccagc gtggctcgtt
     4981 cggccagtta cgcctgtaca accggccggc gatcctcatg ctcaacgacg aggcgggtgc
     5041 gagtcaccag gtcgttctca cggcgctcga cgacgagcgc gcggcgatcg atctcggcgg
     5101 tggcagccgt gaggtcagca tcggcgagtt gtcgcgcttc tggttcggcg atttcgtgat
     5161 gttatggcgg cccggcacga gtcaggtgaa agtgctgtcg gaaggcatgc gcggcgacga
     5221 cgtgcgctgg ctgcgggaga gcctgcagaa gatcgagggc gagcacagcg atcagcccgt
     5281 gagcgatgtg ttcgatgcgg acctgggccg cctggtgcgc gaatttcagc ggcgccatcg
     5341 gctcaccgtg gacgggatcg ccggcgtgca gacacagatc gtgctcgcga gcgccgtcgc
     5401 cccggccgac agtccgttgc tgtacctggc cgacacccac ggcagctgat cgatgtcgtt
     5461 catactcgat gcactgaaga aatccgagag cgaccggcag agacagaccg gtccggcgct
     5521 cttcgaagtc aagattgccc cgcccaagag ccggtttccg ctgtgggcag tgtccattgc
     5581 ggtgttgctc gcggtgaatg ccgcggtggg aggttggctg ctgttacgcc actcatcatc
     5641 gtcaccggcc caggcgcccg ccgcggtccc ggcgttggcg cagtcccctg cgccggtcac
     5701 ctccggcgca gccccgagcg ccgcgctcac gaccgaagcg attaccactg ccccggccgg
     5761 ctctggccca cccgctgcgg caccggccca gccctcgcct gccactctgg ctcagcccac
     5821 gagaacctac tccgccggcg ctggcacggg ccgcaccgat gcggtaggcg cagctaccag
     5881 cccggcgccg atcggagcgg gaggctccgg agagccgacg ctcgccacta acggcaagcc
     5941 ctccgagctc cctcaaggat cgagccagga ggctcccacc gcgaatccgg atgattacgc
     6001 gccggcggca gagccgaagg cgggtccggg gctcgggagc catgtgaagg tcgggacctc
     6061 aagcggctac atcctgtatc aggacgccgc agtcgtgccc ggagccacta
//
LOCUS       DASRHN010000047        12944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_840954, whole genome shotgun sequence.
ACCESSION   DASRHN010000047 DASRHN010000000
VERSION     DASRHN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840954"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1865
                     /locus_tag="VFB37_01755"
     CDS             165..1865
                     /locus_tag="VFB37_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZO21198.1"
                     /translation="MSGSDRLPVVLLWHMHQPQYRDALTGQYVLPWTYLHAIKDYTDM
                     AAHLEAIPNARAVVNFTPVLIEQLEDFAQRVSEHLRSGGPLPDPVLALLGPDPVPRDP
                     AERLDLMRACLRAQRKQMIERFGPYLELATIAETLATPERVAYASNQLIQDLAVWYHL
                     AWLGETVRRTDPLPTALSDQGRGFSPEQRRKLLALIGQQVASIVPRFRRLSERGQCEL
                     SVTPYGHPIVPLLVDFHSARDAVPTMSLPRHAGYTGGVERAAWHMEEGLRVFMRAFGV
                     RPSGCWPSEGAVSRATLEILERFDFRWAATSTNVLRNSLAAVSPQSAEDPLAYNCPYR
                     LPGNTVTTFFRDDALSDLIGFTYATWHGDDAANHLVSELTQLARRYGDSRNHAILIAL
                     DGENAWEHYPFNGYYFLHALYTALASHPSLELTTLSECLTRRMDPIPLSRLVAGSWVH
                     GTLATWMGDPAKNRAWDLLCEAKEAFDRVMQVGAIAAEQRIAAERQLALCESSDWFWW
                     FGDYNPQDAVAQFDRLYRRQLLNLYRVLGLTPPESLSQPISVGRGLPEHGGVMRRANA
                     "
     gene            1865..3214
                     /gene="malQ"
                     /locus_tag="VFB37_01760"
     CDS             1865..3214
                     /gene="malQ"
                     /locus_tag="VFB37_01760"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZO21199.1"
                     /translation="MRLSVFDRRRAGVLLHLGCLDAALGQGGRAFIDWLAEAGFSLWQ
                     ILPLGPTGADGSPYWVRSDFAGHYAFVDPSELPDPHSALFESFLQATTAWLPDYALYE
                     ALSSVQHGKPWWQWPTQLRNREPQAMAQARRELDAPIRRIQLEQFAFSVQWHRLREHA
                     QSRGVRLLGDLPFYVAPDSAETWVHRRQFQLDAAGQPLAVAGVPPDYFSETGQLWGNP
                     LYDWDAIRQDDFALWRARVAGQLERVDALRIDHFRALAAHWAVPAAAADARGGAWKPT
                     PGRELMRLLHAELGDLPIVAEDLGVITPDVEALRRDFRLPGMRVLQFAFGGDADSPHL
                     PYMHEPDSVVYTGTHDNDTTLGWYATLDGESLRRVELFLRLTPGAMPAAMIRAALGSV
                     GRLAVIPLQDVLRLGSEARLNTPGTAQGNWTWRLPPGTLTPQLATQYRLLNGMYGRA"
     gene            3207..3656
                     /locus_tag="VFB37_01765"
     CDS             3207..3656
                     /locus_tag="VFB37_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HZO21200.1"
                     /translation="MHKRVSAWCALPLLLLCGCSLFVPKLEPPKLSILNIQLLRSDLW
                     HQDLRVRMRVENPNDRKLPVRSITYTVDIEGQEFAHGESASSFVVPPLGEAEFDMTVS
                     ANMAGMLVKLLGQGGSPIEYHLSGKVALSEGLLRSIPFDNHGTFKLQ"
     gene            complement(3719..5650)
                     /locus_tag="VFB37_01770"
     CDS             complement(3719..5650)
                     /locus_tag="VFB37_01770"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HZO21201.1"
                     /translation="MTPRLLILLSCALALLLGACGGSSGSGNPSSPMQPNPPPGSTAP
                     SITTQPTDLTVPVGQTATFQVVANGSTPLTYQWYRNGTAISSATAATYTTAPVMTSDN
                     GAKFYVIVGNSEGSTTSNTVTLTVGAAASAADVLTFKNDTARTGQNLAETTLTPANVT
                     SSTFGLLRVLAVDGKVDAQPLYLSQLAIAGGTHNVLLVATENDSVYAFDADTGSSLWH
                     VSLMKPGETPSDTHGCGQVVPQIGITSTPVIDRTAGAHGALYVVAMSLDSSSTYHQRL
                     HALDVTSGAELFGGPTEIAASFTNTAGTTTFSAGQYEERAALLLANGVVYTSWSSHCD
                     DAPYGGWIIAYNKSTLAQTSALNVAPGSGGALSGGSHYDSNGPAIWMSGDGPAVDSAG
                     NVYLLTGNGRFETTLNASGFPNQGDFGNSFLKIAGGSGTLSVADYFAMSREVSESASD
                     DDLGSGGEMLLPDLTDSTKTVKHLVVGAGKDGNIYIVDRDNMGKFDANANHIWQEIDG
                     VVVGAVFSSPAYFNSRVYYGDSNGTLKAFAISNAKLSTTPSSQSAATFPFPGTSPSVS
                     ANGSANGIVWTHENTDPAVLHAFDASNLAHELYNSNQASGARDQFGSGNKFITPTVVG
                     GKVFVGTTSGVAVFGLLGH"
     gene            complement(5784..7238)
                     /locus_tag="VFB37_01775"
     CDS             complement(5784..7238)
                     /locus_tag="VFB37_01775"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HZO21202.1"
                     /translation="MKLTVCVLASEAAPFSKTGGLADVSAALSKYLHSAAHDVKLFTP
                     LYSSIDRRAHPMSAVEGLQNLEIVVGSHRYPMSVLSSPMPGSGVPVLFVDCPELFARG
                     VLYTTDPDEHVRFLAFTRAVFACCQHLKWAPQILHCNDWHTAFGPLLLKAAYSWDTLF
                     RDTRSVLTIHNIGYQGVFNARFSADLALGDKAYLLHQDDLRAGRINPLRHGIMYADAL
                     TTVSPTHAREICSDEFGMGLQDSLRARRDVLTGILNGVDYQEWDPRHDRYLPRHFDPG
                     TLPVKAQLKKQLLARLKLKTPPTTPLAGMVSRLASQKGIELMFDSLPQVLDWRELAFV
                     ALGSGDAPYESFLAQLEADYPGRVVYRRGYDDELAHWIEGACDMFLMPSRYEPCGLNQ
                     MYSLRYGTVPVVRRTGGLADSVEPYDPQTGHGTGVVFNDFDSEALEWALNTALDLYAE
                     PRQWKRLVRNGMSCDFSWQRQGALYVEVYQRALT"
     gene            7237..7395
                     /locus_tag="VFB37_01780"
     CDS             7237..7395
                     /locus_tag="VFB37_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21203.1"
                     /translation="MSNLPVYDVAVSMRRILVGLVLVLLLAVSIGIGVAASRWPTLLR
                     CWHGGRWA"
     gene            complement(7407..8126)
                     /locus_tag="VFB37_01785"
     CDS             complement(7407..8126)
                     /locus_tag="VFB37_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZO21204.1"
                     /translation="MQRQVFTWTTRRLNEPAHMVRWGHFGTPVLLLPSAGGDCEEIER
                     FGLIGALGELIGQGRIKIYSIDGLPVRTWLGNASPQECVRFEKRHDAFLCREVVPRIC
                     ADCQVDSIAPVIAGVSLGAFTAVAEICRHPEVFRAAIGVSGVYDLARHLRAASSEKIR
                     AFSAAALLRLGSKLEPLRRRMIVLASGEGPHETPAESRTLAAALEAQRIPSRLSLWGP
                     AHDHVWASWRAMLPQLLSEQL"
     gene            8241..9173
                     /locus_tag="VFB37_01790"
     CDS             8241..9173
                     /locus_tag="VFB37_01790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZO21205.1"
                     /translation="MSSATANFVDSRDFARIARTIGYIDEHYREQPRLATLAAQAGLS
                     EFHFNRLFRRWAGVTPKQYLAFVTGNTAKDALRTQPSVLDAAYSVGLSGPGRLHDLIV
                     TLDAMTPGELKAQGRGTLIRHGFSPTPFGTALLATTARGICQLAFVEAGEERAAVRDL
                     QQRWPDARLDRDDGYARTLANKVWAPVGGTSSARSSGPLRVSVPGTNFQLKVWQALLD
                     LGAHRATTYTALSEAIRQPGGTRAVGQAVGANPVAWLIPCHNVLRKSGELGGYHWGVQ
                     RKRAMQAWQSLPGLARTQSPLRLDPRHSPPAAAS"
     gene            9365..10882
                     /locus_tag="VFB37_01795"
     CDS             9365..10882
                     /locus_tag="VFB37_01795"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZO21206.1"
                     /translation="MSASPYKQLEQEFKRLYAFRGALSLLRWDAAVMMPRGSADVRGE
                     QLAALETEHHALLTAPRITRLLDRAQANSQGMMDWQIANLREMRRQRDHAIATPVTLI
                     SRLTKAAARAEARWLEARQQGKFELFAPHLEEVVHLVRDKAALLGQALNLAPYDALVD
                     EFSPGVTTADIDSMFKALSRRLPSLIREVISLQEGRSLPPLTGKFPPGKQKALILEVM
                     KAVGFPFDRGRLDESEHPFTEGVPGDIRVTTRLDPNEPFSGLLGALHETGHAMYDLGL
                     PQDWRDQPVGRDRGMALEESQSLLIEMIICRSRPFVRYVQPLLAKHFGVSGPEWDVEN
                     LYAHLTTVRRGLIRVDADELTYPLHIMLRYELEKDLLSGSLAVRDLPEAWNAGMEQRL
                     GIRPSNDADGCLQDIHWAVGSFGYFPSYALGAVIAAQLYETLRADVPELDEQLALGDY
                     SGLFGWLRTQVHGLGAKVPVQDLLKAATGRTLSAVPFVRYVESKYLESAQNIAAA"
     gene            10929..11738
                     /gene="ttcA"
                     /locus_tag="VFB37_01800"
     CDS             10929..11738
                     /gene="ttcA"
                     /locus_tag="VFB37_01800"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HZO21207.1"
                     /translation="MSESIARPSRTFHRLQAHLRGRVGKAIEDFRMISAGDRVMVCVS
                     GGKDSYTLLDILLSLQRSAPVHFDLIAVNLDQKQPGFPAHVLPEFLGKLGVEYRIIEQ
                     DTYSVVKRVLGPEQTMCGLCSRLRRGALYRFAAENGVTRIALGHHRDDIVETLFLNLF
                     FGGRLKAMAPKLLSEDRRHIVIRPLAYVAERDIERYARGRAFPIIPCKLCGSQDNLQR
                     VAIKKMLTDWEREFPGRTESIFSSLRHVELAHLADTRRFDFQALDPGASRP"
     gene            11820..>12944
                     /locus_tag="VFB37_01805"
     CDS             11820..>12944
                     /locus_tag="VFB37_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZO21208.1"
                     /translation="MNAPAALPPLPMHNCYWVVPGLVLAGEHPGGPTPARTKQRLDRL
                     LSAGIGAFLDLTHPEEVPRYDQELPFSIDYRRHAIVDHGLPASPAHMIEILDSVQECL
                     SARRPLYVHCRAGIGRTGIVMGCLLAERGLSGDEALRELNRLWQQCRRSKNWAFVPET
                     DDQVNYIRSWQGRALRAAKLPSKAPVRVRAPVRVQAPVSRTADVPPPAPLSPAAPSRE
                     RPAVLKAPAPRVVSDPLLEPDTLSAARGLRERFMGALLGLAVGDAVAAATQFGRPGSF
                     RPVGDMLGGGPFDLPRGGWSDDTAMALCLADSLLERNGFDARDQVTRYTRWQQDGYLS
                     ATGHAVGITASTARALAMSKWRRQMFAGSHDPKLLDPEPLS"
BASE COUNT         2181 a         4271 c         4208 g         2284 t
ORIGIN      
        1 tcccagtgcg tgatccaggg cgccatcatc gatgagggct gcgaaatccc taacggcatg
       61 cgtattgggg tggaccggca gcaggacgcg cagcgcttcc atgtcaccga gaagggggtc
      121 gtcctcgtta cccccgacat gctgcgcaag atccagacgc cgcaatgagc ggctccgacc
      181 gccttcctgt cgtgctgctg tggcacatgc atcagccgca gtatcgcgat gcgctcaccg
      241 gtcagtacgt gctgccctgg acgtacctgc acgcgatcaa ggactacacg gacatggctg
      301 cgcacctcga ggccatcccg aatgcgcgtg ccgtcgtcaa cttcacgccc gttctcatcg
      361 agcagctcga agacttcgcg cagcgcgtga gcgagcatct gcgttccgga ggaccgctgc
      421 ccgatccggt gctcgcgctg ctgggccctg atccggtgcc gcgagatccc gccgagcggc
      481 tggatctgat gcgcgcctgc ctgcgggcgc agcgcaagca gatgatcgag cgcttcggtc
      541 cctatctgga gctggccacg atcgccgaga cgctcgccac gccggagcgc gtcgcatatg
      601 cctcgaacca gctcatccag gacctggcgg tctggtatca cctggcctgg ctcggcgaga
      661 cggtccgccg gaccgacccc ttgccgaccg cgctgtccga ccagggccgt ggcttcagcc
      721 ccgaacagcg ccgcaagctt ctcgccctga tcggccagca ggtggcctcc atcgtgcccc
      781 gctttcgacg cctgagcgag cggggacagt gcgagctgtc cgtgactccg tacgggcatc
      841 ccatcgtgcc gctgctggtg gactttcact cggcgcgcga tgccgttccc acgatgagcc
      901 tgccacggca cgcggggtat accggcggcg tggagcgagc cgcctggcat atggaagaag
      961 gcttgcgcgt gttcatgcga gccttcgggg tgcgcccgtc gggctgctgg ccctccgagg
     1021 gtgccgtcag ccgggcgacg ctggagattc tcgagcgctt tgactttcgg tgggctgcga
     1081 ccagcaccaa cgtgctgcgc aacagcctcg cggccgtcag tccgcagtcc gccgaagatc
     1141 cgctcgccta caactgtccg taccgcctgc ccgggaatac ggtcacgacc ttcttcaggg
     1201 acgatgctct ctcggacctg atcggcttca cttacgcgac ctggcatggc gacgatgccg
     1261 ccaatcacct ggtgagcgag ctcactcagc tcgcccgccg ctatggcgac tcacgcaatc
     1321 acgccattct gattgccctg gatggtgaga atgcctggga gcactatcct ttcaacgggt
     1381 actacttcct gcatgcgctg tacaccgcgc tcgcgagcca tccatcgctc gagctgacca
     1441 cgctttcaga gtgcctgacc cggagaatgg acccgatccc actctcacgt ctcgtcgcgg
     1501 gaagctgggt gcacggcaca ctggcgacct ggatgggcga tcctgcgaag aaccgggcgt
     1561 gggatctgtt gtgcgaggcc aaggaagcgt ttgatcgcgt catgcaggtc ggggcgatcg
     1621 ccgccgagca gcgcatcgcg gctgagcggc agctggccct gtgcgagagt tcggactggt
     1681 tctggtggtt cggcgactac aatccgcagg atgcggtcgc tcagttcgat cggctctatc
     1741 gccgccagct cctgaatctc tatcgggttc tcggtctgac gcccccggag agtctgtcgc
     1801 agcccatttc ggtcggccgc ggcctgccgg agcacggggg cgtcatgcgt cgcgccaacg
     1861 cgtgatgcga ctgtcggtat tcgatcggcg gcgcgccggt gtgctgctgc atctcggctg
     1921 cctggatgcg gccctcggtc aaggcggccg cgcgttcatc gactggctcg ccgaggccgg
     1981 cttcagcctc tggcagattc tgccgctcgg gcccaccggc gcagacggct cgccctattg
     2041 ggtgcgctcg gactttgccg gtcactacgc tttcgtagac cccagcgagc tgcccgaccc
     2101 gcattccgca ctttttgaat ccttcctgca ggccacgacc gcctggctac cggactatgc
     2161 gttgtacgag gcgctcagct cggtccagca cggcaagccc tggtggcaat ggcccacgca
     2221 actgcgcaat cgcgagccgc aggcgatggc gcaagctcgt cgggagctcg atgcgccgat
     2281 ccggcgaatc cagctcgagc agttcgcctt cagtgtccag tggcaccggt tgcgtgagca
     2341 cgcgcagtcg cgtggcgtgc gcctgttggg cgatctgccc ttctatgttg cccccgattc
     2401 cgccgaaacc tgggtccatc gccgtcagtt ccagctcgat gccgcggggc agcctttggc
     2461 cgtggccggc gttccaccgg attacttctc ggagaccggg cagctctggg gcaatcccct
     2521 ctacgactgg gatgccatcc gccaggacga cttcgcgctg tggcgggcgc gggtggccgg
     2581 gcagctcgag cgcgtggacg ccctgcgcat cgatcacttc agagcattgg cggcacactg
     2641 ggcggtgccc gccgctgccg ccgatgcacg cggcggcgcc tggaaaccca ctcccggtcg
     2701 ggagttgatg cgactgttgc acgctgagct cggcgacctg ccgatcgtgg ccgaggatct
     2761 gggtgtgatt accccggatg tcgaagcctt gcgccgggac ttccgcttgc ccgggatgcg
     2821 cgtactgcag ttcgcgttcg gcggcgatgc cgatagcccc catctgccgt acatgcacga
     2881 gccggacagt gttgtgtaca ccggtacgca cgacaacgac acgacactcg gctggtacgc
     2941 gacgctcgat ggcgagagcc tgcgccgggt ggagctcttt cttcggttga cgccgggggc
     3001 catgcccgcg gcaatgatcc gggcggcgct gggatcggtc gggcggctcg cggtcatccc
     3061 gctgcaggat gtgctgcgcc tcggctccga agcccggctc aacacgcccg gcacagcgca
     3121 gggcaactgg acctggcgcc tgcctccagg caccctcacc ccgcaactcg ccacccagta
     3181 tcggttgctg aacgggatgt atggtcgtgc ataaacgcgt gtccgcctgg tgcgctttgc
     3241 cgctcctgct gctgtgcggc tgctccctgt tcgttccgaa gctggagccc ccgaagctct
     3301 ccatcctcaa catccagctt ctcaggagcg atctgtggca ccaggacctg cgggtgcgca
     3361 tgcgcgtgga gaacccgaac gatcgcaagc tgccggttcg cagcatcacc tacacggtgg
     3421 acatcgaagg acaggaattt gcgcacggcg agtcggcttc gagcttcgtc gtgccgccgc
     3481 ttggggaagc ggaattcgac atgaccgtct cggccaacat ggccggcatg ctggtcaagc
     3541 tgctcgggca gggcggcagc ccgatcgaat atcacctgtc cggcaaggtg gccctgtcgg
     3601 aggggctgct gcgctcgatc cccttcgaca accacgggac cttcaaactg cagtagccag
     3661 gcaccgcgcg cgagaagggc gagccgcgcg tcccggaaga cgcgcggccc ggataccgtc
     3721 aatgtccgag gagcccaaac acggccacgc cgcttgtcgt ccccacgaag accttgccac
     3781 cgacgaccgt gggggtgatg aacttgttgc ccgaaccgaa ttggtcccgg gccccggagg
     3841 cctggttgct gttgtagagc tcatgcgcga gattgctcgc atcaaaggcg tgcagcaccg
     3901 ctggatcggt gttctcgtgc gtccagacga tcccgttcgc tgagccgttc gcggagaccg
     3961 agggggaggt cccgggaaac gggaacgtcg ctgcagactg ggaggagggc gtcgtggaca
     4021 gcttggcatt cgagattgcg aacgctttga gcgtgccgtt cgaatcgccg taatacaccc
     4081 tgctgttgaa gtaggccgga gaggagaaca cggcgcccac caccacccca tcgatctcct
     4141 gccagatgtg gttggcgttg gcgtcgaatt tccccatgtt gtcccgatcg acgatgtaga
     4201 tattcccgtc cttgccggcc ccgacgacca ggtgtttgac cgtcttggtg ctgtcggtca
     4261 ggtccgggag cagcatctcc ccgcccgagc ccagatcatc atcgctcgcc gattcggaca
     4321 cctcacgcga catggcgaag tagtcggcca cgctgagcgt gccgctcccg ccggcgatct
     4381 tgaggaagga gttgccgaaa tcgccctgat tgggaaatcc gctagcattg agcgtcgtct
     4441 cgaagcggcc gttgccggtc aggagataga cgtttcccgc gctgtccacc gccggaccgt
     4501 cgccgctcat ccagatggcc ggaccgttgc tgtcgtaatg actgccaccg ctcagggcac
     4561 ccccgctgcc gggcgccacg ttcagggccg aggtctgcgc gagcgtgctc ttgttgtagg
     4621 cgatgatcca accgccataa ggagcgtcgt cgcagtgcga gctccaggag gtatagacca
     4681 cgccattcgc caggagcaac gctgcccgct cttcgtactg tcccgcgctg aaggtcgttg
     4741 tcccggcggt gttggtgaac gaggctgcga tctcggtggg accgccgaac aactccgctc
     4801 cgctcgtcac atcgagtgca tgcagtcgct ggtgataggt cgatgaggag tctagcgaca
     4861 tggccacgac gtacagggcg ccgtgcgctc cggcggtgcg atcgatcacc ggtgtcgagg
     4921 tgatgccgat ctgcggcacg acctgcccgc acccatgagt gtcgctgggg gtctcgccgg
     4981 gcttcatcag cgagacgtgc cacagactcg agccggtgtc ggcgtcgaag gcgtaaacag
     5041 aatcattttc cgtggcgacc aacaggacat tgtgcgtacc gcctgcaatg gccagctggg
     5101 acaggtaaag cggttgcgca tccactttgc cgtcgacagc gaggacgcgc agcaacccga
     5161 aggtgctcga ggtgacgttg gcgggcgtga gagtcgtctc ggcaaggttc tggcccgtgc
     5221 gcgccgtatc gttcttgaaa gtgagtacat ccgcagcgct ggccgcggcg cctaccgtca
     5281 gcgtcaccgt gttgctcgtg gtggagcctt cactgttgcc gacgatcacg tagaacttgg
     5341 cgccgttgtc gctcgtcatt acgggtgccg tcgtgtaggt cgcggcggtg gcactcgaga
     5401 tggcggttcc gtttcgatac cactgatagg tgagtggggt ggaaccgttc gcgacgacct
     5461 ggaacgtggc agtttgtccg acaggaacgg tgaggtccgt gggctgcgtg gtgatggagg
     5521 gcgccgtgga gcccggcgga ggattgggct gcatgggtga gctcggatta cccgaccccg
     5581 aagagccgcc acaggcaccc agcagcaacg cgagtgcgca ggagagaagg atgaggagtc
     5641 gaggagtcat gctgatgctg ccccgcgcga gctggccctg tcgcccgcgg ccgctcgccg
     5701 agcccaggat tacagagaaa ccggctgccg ctcaactttc ggccaatggc gacaaacggt
     5761 cgggcggata ccgcggttcc ctctcaggtc agcgcgcgct ggtagacctc gacgtacagc
     5821 gcgccctggc gctgccagga aaaatcacag ctcatgccgt tgcgcacgag gcgtttccac
     5881 tgccgcggct ctgcgtacag atcgagcgcc gtgttgagcg cccactcgag cgcctcggag
     5941 tcgaaatcgt tgaacacgac accggtgccg tgcccggtct gcgggtcgta gggctccacc
     6001 gagtcggcca agccacccgt gcggcgcacg accggcaccg ttccataacg caagctgtac
     6061 atctggttga gcccgcacgg ctcatagcgt gacggcatga gaaacatgtc gcacgcgccc
     6121 tcgatccagt gcgccagctc atcgtcgtag ccgcgccgat agacaacccg ccccggatag
     6181 tccgcttcga gctgcgccag gaaactctca tagggcgcat cgccgctgcc cagcgcaacg
     6241 aaggcaagct cgcgccagtc gagcacctgc ggcagcgagt cgaacatcag ctcgatgccc
     6301 ttctgggaag cgagccggct caccatgccg gctagcggcg tcgtgggagg cgttttcagt
     6361 ttcaaacgcg ccagcaactg tttcttgagc tgcgctttga ccgggagcgt cccgggatcg
     6421 aagtggcgcg gcagataccg gtcatgccgc ggatcccact cctggtaatc cacaccattc
     6481 aggatcccgg tcagcacgtc gcggcgcgcg cgcagcgagt cctgcaaacc catgccgaat
     6541 tcatcgctgc agatctcgcg cgcgtgcgtt ggactgaccg tggtgagggc atccgcatac
     6601 atgatgccgt ggcgcagcgg gttgatgcgg ccggcgcgaa gatcatcctg gtgcagaaga
     6661 taagccttgt ccccgagcgc cagatccgcg ctgaagcggg cattgaagac accctggtag
     6721 ccgatgttgt ggatggtgag gacggagcgg gtatcgcgaa agagcgtatc ccagctgtag
     6781 gcggccttca gcagcagagg accgaaggcg gtatgccagt cattacagtg cagaatctgc
     6841 ggcgcccatt tgaggtgctg gcagcaggcg aatacggccc gggtgaaagc caggaagcgc
     6901 acatgctcat ccggatcggt cgtatagagc accccccggg cgaacagctc gggacagtcc
     6961 acaaacaata ccgggacgcc cgagccgggc atcggtgagc tcagcacact catcggatag
     7021 cggtgcgagc ccaccacgat ctcgagattc tgcagtccct ccaccgcact catcgggtgc
     7081 gcgcgccggt cgatggagga atacaggggc gtgaataact tgacgtcatg agctgcggaa
     7141 tgcaggtact tgctcagagc cgcagacaca tccgccagac cgcccgtctt ggaaaaggga
     7201 gccgcttccg aggccagaac gcacacagtc agtttcatga gcaatctgcc ggtttatgat
     7261 gtagccgtgt caatgcgccg cattctcgta ggactcgttc tggtgctgct gctcgcggtg
     7321 tcgatcggca tcggcgtggc ggcttcgcgc tggccaacgc tgctgcggtg ctggcacggc
     7381 ggccgctggg cctgaggcgc ggtccttcag agttgctccg acaataactg cggcagcatg
     7441 gctcgccagc tggcccagac gtgatcgtga gccggacccc agaggctgag ccgcgagggg
     7501 atccgctgag cctccagggc cgcagccagg gttctcgact ctgccggggt ctcgtgaggc
     7561 ccctcgccgc tggccaacac gatcatccgg cgccgcagcg gctcgagttt cgagccgagc
     7621 cgaagcaagg ccgccgcgga aaatgcccgg atcttctcac tcgaggccgc ccgcaagtgc
     7681 cgcgcgaggt cgtacacgcc actcacaccg atcgcggcgc ggaatacttc cggatgccgg
     7741 cagatctccg caacggccgt gaacgcgccg agagagacgc cggcaatgac cggagcgatc
     7801 gaatcgacct gacagtctgc acagatacgc ggcacgactt cccggcacag gaaggcatca
     7861 tgacgcttct cgaagcgcac acactcctgg ggcgaagcgt tgcccagcca ggtccgcacc
     7921 ggcagaccgt cgatcgaata aatcttgatc cgcccctggc cgatcagctc cccgagcgcg
     7981 ccgatgaggc cgaatcgctc gatctcctca cagtcgccgc cggcactcgg cagcagcagc
     8041 accggcgtgc cgaaatggcc ccaacgcacc atgtgtgccg gctcgttcag gcgccgcgtc
     8101 gtccacgtaa acacttgtct ttgcatcggc cgtccagtgt agctgatgag gtgcgagcgc
     8161 aagagccggc ggcccacccc agcaagcgcg gccgcaagaa tcgggtcgcg gctgcgggcg
     8221 cgctccgtat gatgtggccc atgagctcag ccaccgccaa tttcgttgac agccgcgact
     8281 tcgcccgcat cgcccgcacg atcggttaca tcgacgagca ctaccgcgag cagccgcgcc
     8341 tggccaccct cgccgcgcag gccggcctgt cggaattcca cttcaaccgg ttgttccgac
     8401 gctgggcggg cgtgacgccc aagcaatacc tcgccttcgt gacaggcaac acggcgaagg
     8461 atgcgctcag gacccagcct tcggtgctgg atgccgccta ttcggtcggc ctgtcaggcc
     8521 cgggacgatt gcacgacttg atcgtgacgc tcgatgcgat gacaccggga gagctcaagg
     8581 cacagggccg cggcaccctg atccgccatg gattcagccc caccccgttc ggcacggcat
     8641 tgctcgcgac gacggcccgc gggatctgcc agcttgcctt cgtggaggct ggggaggagc
     8701 gggccgcggt gcgcgatctt cagcagcgct ggcccgacgc ccggctggat cgcgatgacg
     8761 gctacgctcg caccctcgcc aacaaggtct gggcgccagt cggcgggaca agctccgcgc
     8821 gctcttccgg gcccctgaga gtgagcgtgc cgggcacgaa cttccaactc aaggtctggc
     8881 aggcgctgct ggatctgggc gcgcaccgcg cgaccactta caccgctttg tccgaggcca
     8941 tccgccagcc cggcggcacc cgagcggtgg ggcaggccgt gggcgcgaat ccggtggcgt
     9001 ggctcattcc ctgtcacaac gtgctgcgca agagcggcga gctcggcggc tatcactggg
     9061 gcgtgcagcg caagcgcgcc atgcaggcct ggcaatccct gcccggcctc gcgcggacgc
     9121 agtctccgct tcgcctcgac ccgagacatt ccccaccagc ggcggccagt tagacagctg
     9181 catatgtaaa caaaagcacg gattccgggc cgggagctgt caaccacccc ctccgtagct
     9241 taggtgattg ttttagaagg aaaaatagta gcaggcgacg gcttggccgt ggccccgctg
     9301 gtaaactggc tgccatcccg ctcccgatcc acgcttctgt ggatcgcgac ccgagtacca
     9361 gacgatgtca gcttcgcctt ataagcaact cgaacaggag ttcaaacgcc tgtacgcctt
     9421 ccgcggtgcc ctgtcgctgt tgcgatggga tgccgccgtc atgatgccgc gcggcagcgc
     9481 cgatgtgcgc ggtgagcagc tggcagcgct cgagaccgag catcacgctc tgctcacggc
     9541 cccgcgcatc acccgcctcc tggatcgggc ccaggcgaat agccagggga tgatggactg
     9601 gcagatcgcc aatctgcgcg agatgcgccg ccagcgcgat catgcgattg ccaccccagt
     9661 caccctgatc tcgcggctca ccaaggcggc cgcacgggcg gaagctcgct ggctcgaagc
     9721 gcgccagcag ggcaaattcg aattgttcgc cccgcacctg gaagaggtgg tgcatctggt
     9781 ccgcgacaag gcggcgctgc tcggtcaggc actgaatctc gccccttatg atgcgctggt
     9841 ggatgagttc tctccgggcg tgaccacggc ggacatcgac tcgatgttca aggcgctctc
     9901 gcgccgtctg ccatccctga tccgcgaggt gatctcgctc caggagggcc ggtcgctgcc
     9961 gcccctgacc ggcaaattcc cgcccggcaa gcagaaggct ttgatcctcg aagtgatgaa
    10021 ggcggtgggc tttcccttcg atcgcggcag gctggatgag agcgagcacc ccttcaccga
    10081 gggggtgccc ggggatatcc gggtcaccac gcggctggat ccgaacgagc cgttctccgg
    10141 gttgctcggc gcgctgcacg agaccggcca tgccatgtat gacctcggcc tcccgcagga
    10201 ttggcgggat cagcccgtcg ggcgtgatcg gggcatggcg ctcgaggaga gccagtcgct
    10261 gctcatcgag atgatcatct gccgcagccg gcccttcgtt cgatacgtgc agccactgct
    10321 tgcgaagcac ttcggcgtga gcggtcccga gtgggatgtc gagaatcttt acgcccatct
    10381 gacgacggtg cggcgtggat tgatccgagt cgatgccgat gagctcactt acccgctgca
    10441 catcatgctg cgctatgagc tcgagaagga tctcttgagt ggcagtctgg cggtacgcga
    10501 tctgcccgaa gcctggaacg ccgggatgga gcagcgcctc gggatccggc cgagcaacga
    10561 tgcggacggg tgtctgcagg acatccattg ggcggtcggc tcgtttggct acttcccctc
    10621 gtacgccctg ggtgcggtga tcgccgctca gctctatgag acgctgcgcg ccgatgtacc
    10681 ggagttggac gagcagctcg cgcttggcga ttactcggga ctgttcggct ggttgcgcac
    10741 ccaggtgcac ggcctcggag caaaggtgcc ggtgcaggat ctgctgaagg cggcgacggg
    10801 ccgaacgctc tcggccgttc ccttcgtgcg ttatgtcgag tcgaagtatc tcgagagcgc
    10861 gcagaacatc gccgccgcct gaagaccgtc gagcttcccg tcatccgccg ctggtaggat
    10921 cactccccgt gagtgagtcc attgcaaggc cttccagaac cttccatcgg ttgcaggcgc
    10981 acctgcgtgg ccgcgtcggc aaggcgatcg aagatttccg catgatcagc gcaggcgacc
    11041 gcgtcatggt gtgcgtgtcc ggcggcaagg attcctacac gctgctcgac attctgcttt
    11101 cgctccagcg cagcgcgccg gtgcacttcg atctgatcgc agtcaacctc gatcagaagc
    11161 aacctggctt ccctgcgcac gtgctgccgg aatttctcgg caaactcggt gtcgaatacc
    11221 gcatcatcga gcaggacacc tacagtgtcg tgaagcgggt gctgggcccc gagcagacga
    11281 tgtgcggact ctgctcccgg ttacgccggg gcgctctgta tcgcttcgca gccgagaacg
    11341 gcgtgacccg gatcgccctc ggtcatcatc gcgatgacat cgtcgagacg ctctttctca
    11401 acctgttctt tggcggccgg ctcaaagcca tggcgccgaa gcttctttcc gaggaccgcc
    11461 gccacatcgt gatacgccca ctggcctatg tggctgagcg cgacatcgag cgttacgctc
    11521 gcggccgggc cttccccatc atcccgtgca agctgtgcgg gtcgcaggac aatctgcagc
    11581 gggttgcgat caagaagatg ctgaccgatt gggaacggga attcccgggt cgaaccgaat
    11641 ccatcttctc ttcgctgcgg cacgtggagc tcgcgcatct cgccgacacc cggcggttcg
    11701 atttccaggc gctggatccc ggcgcgtccc ggccatgacg gagcgtgagg agcaggcact
    11761 cgaagagcgc ctcgacgcgc tcgccacccc agcctgatcg gcgcaaccgc cccttacgca
    11821 tgaacgcgcc tgctgccctg ccgccgctgc caatgcacaa ctgctactgg gtcgtgcccg
    11881 ggctggtgct ggcgggtgag cacccgggtg gcccgacacc agcccgtacg aagcagcgac
    11941 tcgatcggct cctctcggcg ggcatcggcg cgttcctgga tctgacccac ccggaggagg
    12001 tgccgcgcta cgaccaggag ctgccgttca gcatcgacta tcgccgccat gcgatcgtgg
    12061 atcacgggct gccggcgagt cccgcgcaca tgatcgagat cctcgactcg gtccaggaat
    12121 gcttgagtgc gcgacggccg ctctatgtcc actgccgcgc gggcatcggg cgcaccggga
    12181 ttgtgatggg ctgcctgctg gccgagcggg gcctgagcgg cgatgaggcg ctgcgcgagc
    12241 tgaaccgact ctggcagcaa tgccggcgct cgaagaactg ggcattcgtg cccgagacag
    12301 acgatcaggt gaattacatc cggagctggc agggacgggc tctgcgggcc gccaagctcc
    12361 ccagcaaagc gccggtgcgc gtgcgagccc cggtacgcgt gcaagccccg gtctcccgaa
    12421 ctgcagatgt cccgcccccg gcacccctgt caccggcggc gccttcgcgt gagcggcccg
    12481 cagtgctgaa agccccggct cctcgggtcg tgtccgatcc gcttctcgag cccgataccc
    12541 tgagcgcggc tcggggctta cgcgagcggt tcatgggcgc gcttctgggc cttgcggtcg
    12601 gggacgcggt ggccgccgcc acccaatttg gccggccggg atccttccgt cccgtcggag
    12661 acatgctggg tgggggaccc tttgatctgc cccggggagg ctggagcgat gacaccgcaa
    12721 tggcgttgtg tctggcggac agcttgctcg aacggaacgg gttcgacgcc cgcgatcagg
    12781 tcacccggta cacacggtgg cagcaggacg ggtatctatc tgcgactggg catgccgtgg
    12841 gcatcacggc cagcaccgcc cgcgccctgg ccatgtccaa gtggcgccgg cagatgttcg
    12901 caggctccca cgatccgaag ctcctggatc cagaaccgtt gtcc
//
LOCUS       DASRHN010000048         8473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1499467, whole genome shotgun sequence.
ACCESSION   DASRHN010000048 DASRHN010000000
VERSION     DASRHN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499467"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFB37_01810"
     CDS             complement(<1..437)
                     /locus_tag="VFB37_01810"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HZO21209.1"
                     /translation="MGTSELGVLIPIIAVTLGLGCAIVAVVSKHRHRLHELEFRHRER
                     MAAIDKGLELPPEPTQDGYAADYWKHARSGPRVLLKGLVWLGVGIALVLPGDPFGSDH
                     HFWTFGWIAVAVGAAYLIYYFVEARHRASPGQGFPGGSGSASNP"
     gene            714..1241
                     /locus_tag="VFB37_01815"
     CDS             714..1241
                     /locus_tag="VFB37_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO21210.1"
                     /translation="MLTDRGVTELVQSGHRDEALAVVVRQLRRKVFGLAYSYLHNRDA
                     AEDVAQEVFIKVWRALPAFDGRAALSTWVYAIARNASLSALRMRRPQVSLSDSAVAAQ
                     IDSASAATADEAQLDAAALRRLVEQLPLKQRQVVTLFYMQEQSHEEVAAMLAMPVGTV
                     KTLLHRARDRLRQIT"
     gene            1238..1687
                     /locus_tag="VFB37_01820"
     CDS             1238..1687
                     /locus_tag="VFB37_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21211.1"
                     /translation="MSFPDSADPASFDERLSRAFAGLDVSSDFDALLTARLARESEQD
                     FAQRLHLARQLELERYRNARFHASWRQEARALLRLLTSETAGVAVLIVLVGSAVWGPL
                     AAWANVQWPGLSDALHQNAAIVWPSLLGVLFGLAPLLARRLRDLRAD"
     gene            complement(1724..2644)
                     /gene="folD"
                     /locus_tag="VFB37_01825"
     CDS             complement(1724..2644)
                     /gene="folD"
                     /locus_tag="VFB37_01825"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZO21212.1"
                     /translation="MSGVEAGAATAADISGATRAPGGAILLDGKAVGARVRLEVAQRA
                     AEFSDRFGRRPGLAVVQVGQDPASSVYVRSKRKSSVEAGIESYAHDLPATASQSELLE
                     LVRSLNHDNRVDGILVQLPLPKGLDANLIMDAVNPAKDVDGFHPVNTGLLAQKRPGLR
                     PCTPFGVIRLAQAYGIALTGMRATVVGASNIVGRPMALELLLARATVTVCHTGTRDLR
                     AEVERADVVVAAVGKPGFVHGDWIRPGAAVFDVGINRSESGKLVGDVEFERASERAGW
                     ITPVPGGLGPMTIAMLLSNTVDAACRRHGM"
     gene            complement(2679..4100)
                     /gene="ahcY"
                     /locus_tag="VFB37_01830"
     CDS             complement(2679..4100)
                     /gene="ahcY"
                     /locus_tag="VFB37_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011266417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZO21213.1"
                     /translation="MNANLKQAARNPDHHVADLALADWGRKEILIAETEMPGLIAIRE
                     EFAPLQPLRGARISGSLHMTIQTAVLIETLQALGAQVRWASCNIFSTQDHAAAAIAAR
                     GTPVFAWKGESLADYWEYTHRIFEWTDGGFSNMILDDGGDATLLLHLGARAERDASLI
                     SKPSSDEESALFAAIQGRLRSDPKWYSTRLGHVKGVTEETTTGVHRLYQMAKEGQLAF
                     PAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVALVAGYGDVGKGCAQALR
                     ALSAQVWVTEIDPICALQAAMEGYRVVTLEYAADKADIFVTCTGNFHVITHDHMKRMK
                     NNAIVCNIGHFDNEIDVASLRQYAWENIKPQVDHIIWPDGRRMILLAEGRLVNLGCGT
                     GHPSYVMSSSFANQTIAQIELFTHPERYPVGVHVLPKRLDEKVARLQLKKLNAQLTEL
                     TEVQARYIGVDKAGPYKSEHYRY"
     regulatory      complement(4117..4215)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(4250..5425)
                     /gene="metK"
                     /locus_tag="VFB37_01835"
     CDS             complement(4250..5425)
                     /gene="metK"
                     /locus_tag="VFB37_01835"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZO21214.1"
                     /translation="MANTYLFTSESVSEGHPDKVADQISDAMLDAILREDERGRVACE
                     TLIKTGVVVVAGEVTTKAWVDVEALVRKTVLEIGYNSSEMGFDGASCGVVNIIGKQSP
                     DIAQGVDRKDERKQGAGDQGLMFGYATNETDVLMPAPITLAHRLVKRQAQVRKSGKLA
                     WLRPDAKSQVTLRYEDDHPVGIEAVVLSTQHNPEVSTKALREAVMEEILKPSLPAKWL
                     DKRTKYHINPTGRFVIGGPVGDCGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSA
                     AYAARYVAKNIVAAGLAERCEVQVSYAIGVAEPTSIMVDTFGTSRLSRERLTQLVRKH
                     FDLTPYGLREMLDLVRPIYQKTAAYGHFGRTEAEFSWERTDKAAELADEAKGAARAA"
     gene            complement(5525..6493)
                     /locus_tag="VFB37_01840"
     CDS             complement(5525..6493)
                     /locus_tag="VFB37_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZO21215.1"
                     /translation="MTHPTIEPSRRRVLICGSVAFDTIMVFEDHFSRHILPEKIHILN
                     VSFLVPQLRREFGGCAGNIAYNLKLLGDVAYPMATVGRDFGPYREWMARVGVPADYVK
                     VLESEHTAQAFITTDLDDNQITAFHPGAMQQSHLNRVPDAAEIGMGIVAPDGRQGMLE
                     HAEQFAAARIPFIFDPGQGLPLFDGEELRRFIERSSWVAMNDYEWQLVQQKTGWSVKD
                     VTRRVQALIVTRGAQGSVIYTEREQIEIACVKAKAVVDPTGCGDAYRAGIIHGLMHGL
                     DLRRAGQIASVLGAIKIEYLGTQNHRFTSAELKQRLEQSFGGVASP"
     gene            complement(6490..7677)
                     /locus_tag="VFB37_01845"
     CDS             complement(6490..7677)
                     /locus_tag="VFB37_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21216.1"
                     /translation="MSSQKGLPTSAGGPTGFGPTPFRFYDNRQKYLAFVNTCNEKAAI
                     ARRAARELRLLRPTPPALRVFDAGMGDATVLARLMRSTHFVYPTVPLLVVAKEISLED
                     VRLGLEKMPDRFYEHPATVLVITNLNYADAPQLRPREQTDAAALNWQKIALAGTSADE
                     YAAQIEELGPTLAEGWQTRPSLQSGNPVYVRPSVLVLHRADHEFLLDGVIPQARQVLP
                     GYDLVLASQPWRARMPAQFKARKVLAPLARSLVPGGRLLAIQSYGHDPGLEIIHRLWP
                     GEDPFKVARRELLAALAAELGESAVDYDLSIPEDQASLFRYEMHTLPSEIGDRIGTST
                     LFAAWNAAIYVNQIEDERLDAAVQSNAYLAATQAVLQKYSGLWFNDETFVVSRKPRSE
                     SSR"
     gene            complement(7700..>8473)
                     /gene="fdhD"
                     /locus_tag="VFB37_01850"
     CDS             complement(7700..>8473)
                     /gene="fdhD"
                     /locus_tag="VFB37_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZO21217.1"
                     /translation="VTVDRWVRGAVTHKTDQVAEEIPIALVYHGVPHVVMLATPANLE
                     DFAVGFTLSEGLVARPEEIRSVEVTQGEEAIDVRITVAWDRFSALLQRRRNLTGRTGC
                     GLCGAEKAEDAIREIAPVGAGPSVTVEDLHAAIDQLGTMQPINSRTGSVHAAAWVIPG
                     KGIQLVREDVGRHNALDKAIGALVRGGIDFGSGYMLVTSRASFEMVQKSAAAGVAFLA
                     AFSAPTAFAVRLAEKSGLTLVAFARREQHVVYSHPKRLK"
BASE COUNT         1478 a         2793 c         2638 g         1564 t
ORIGIN      
        1 gggttgctcg cggagcccga accgccaggg aagccctggc cgggggaagc ccgatggcga
       61 gcctccacga aatagtagat caggtaggca gcccccaccg ccacggcaat ccagccgaac
      121 gtccagaaat gatgatcaga gccgaagggg tcgcccggca ggacgagggc gattcccact
      181 ccgagccaga ccagaccctt gagcagaacc cgtgggccgc tgcgggcatg cttccaatag
      241 tcggccgcgt agccgtcctg cgtcggctca ggaggcagct ccaggccctt gtcgatcgcg
      301 gccatgcgct cccgatgccg gaattccagc tcgtgcagcc ggtgtcgatg cttggagacg
      361 accgcgacga tcgcgcaccc gagcccgagc gtcaccgcaa taataggaat caagacaccc
      421 agttccgatg ttcccacggc gctctcccat caactcatga tcatgtgaat gatcgcctcg
      481 atcgagggcc agtgctgatg cacaaagtag cctactatcg cgcccagcaa cgctgctgat
      541 acgttctctt tcacggctgc cccattggcg gctttttcct gcatacgcac ttgatacgtc
      601 caggcgggcg agagggtttc agccttggga attctttccg ccgagctgaa actcccgcgc
      661 cgccagctgc gtatcaatcg agtgatgccc tatgcaagcg ggaagctggt gcggtgctga
      721 cggaccgggg cgtcacggaa ctggtgcagt ccgggcaccg cgatgaggcg ctggcggtgg
      781 tcgtgcgtca gctgcggcgc aaggtgttcg gactcgctta cagctacctg cacaaccgcg
      841 atgcggcgga agacgtggct caggaagtgt tcatcaaggt ttggcgtgcg ctcccggctt
      901 tcgacggtcg cgcggcgctg tccacgtggg tctacgcgat cgcccggaac gccagtcttt
      961 ccgcgctgcg catgcggcgg ccgcaggtat cgctcagcga ttcagcggtc gccgcgcaga
     1021 tcgattcggc aagtgccgcc acggccgacg aagcacagct cgatgctgcc gcgctcaggc
     1081 gtttggtcga gcagctccct ttgaaacagc gccaggttgt gacactcttt tatatgcagg
     1141 agcaatcgca cgaggaggta gccgcgatgc tggcaatgcc ggtgggaacc gtcaagacac
     1201 tgctgcaccg ggcccgcgac cgactgagac agatcacatg agctttcccg attcggcaga
     1261 ccccgcgtca ttcgatgaac gcctgtcacg cgcctttgcc ggactggatg tcagctccga
     1321 cttcgacgcg ctgctgactg cgcgcctggc gcgggaatca gaacaggatt tcgctcagcg
     1381 tctgcatctt gcccggcagc tcgagctgga gaggtatcgg aatgcgcgct ttcacgcaag
     1441 ctggcggcag gaggctcggg cactgctgag actgttgacc tccgagacgg caggcgtcgc
     1501 ggtgctcatc gtcctcgttg gctctgcggt gtgggggcct ctcgcagcct gggcgaatgt
     1561 gcagtggccg gggttgagcg atgcgctgca tcagaatgcg gcgatcgtct ggccttcctt
     1621 gctcggagtc ctttttggtt tggccccgct tctcgcccgg cggttgcgcg acctgcgcgc
     1681 cgattgacat cagcctgcca cgcccttggg atggccccag aatctacatg ccgtgccggc
     1741 ggcaggccgc gtccactgtg ttcgacagca gcatggcgat cgtcatcggc ccgagccctc
     1801 ccggcacggg tgttatccag cccgcccgct cggaggcgcg ttcgaactcg acatcgccca
     1861 ccagcttgcc cgactcggag cggttgatcc cgacatcgaa cacggctgcc ccggggcgaa
     1921 tccagtcgcc gtgcacgaag ccgggcttgc cgaccgccgc aacgacgacg tccgcgcgct
     1981 ccacctccgc ccgcagatct cgcgtacccg tatgacatac cgtcacagta gcccgagcca
     2041 gcagcagctc gagggccatc gggcgaccga cgatgttgga agcaccgacc accgtcgcac
     2101 gcatgccggt gagcgcaata ccgtacgcct gggccaggcg gatcaccccg aacggcgtgc
     2161 acggacgcag accaggccgc ttctgcgcaa gtaaccccgt attgacagga tgaaacccat
     2221 caacgtcctt cgcgggattc actgcgtcca tgatcaggtt ggcatcgaga cccttcggca
     2281 gtggaagctg cacgaggatg ccgtccacac gattgtcatg attgagcgag cgcaccagct
     2341 cgagcagctc cgactggctg gccgtcgccg gcagatcatg cgcataggac tcgatgccgg
     2401 cctccaccga tgacttccgc ttgctgcgga cgtacaccga ggaggccgga tcctgtccta
     2461 cctgcacgac ggcgaggccc ggccgcctgc cgaagcgatc gctgaactca gcggcgcgct
     2521 gcgccacttc caggcgcacc ctggcaccca ccgccttgcc gtcgagcaga atcgcgccgc
     2581 cgggggcgcg cgtcgcgccc gagatgtcgg ctgctgttgc ggcgcctgcc tcaactccgg
     2641 acatgtagct cacctcgcat gcgtggcagg cgtttctctc agtaccgata gtgctcggac
     2701 ttgtaggggc cagccttgtc gacaccgatg taccgggcct gcacctcggt gagctcggta
     2761 agctgcgcat tgagcttctt gagctgcaac cgcgcgactt tctcatcgag gcgcttgggc
     2821 agcacgtgca cgccgaccgg gtatctctcc ggatgggtga acagctcgat ctgcgcaatg
     2881 gtctggttgg cgaagctcga gctcatcacg taactcggat gcccggttcc gcagccgagg
     2941 ttcaccaggc gtccctcggc cagcaggatc atgcgccggc catcgggcca gatgatgtga
     3001 tccacctggg gcttgatgtt ctcccaggcg tactgccgca ggctcgccac atcgatctcg
     3061 ttgtcgaaat ggccgatgtt gcacacgatg gcgttgttct tcatgcgctt catgtgatcg
     3121 tgcgtgatca catgaaagtt gccggtacag gtgacgaaga tatcggcctt gtcggccgca
     3181 tactccagtg tcacgacccg gtaaccttcc atcgccgcct gcagggcgca gatcggatcg
     3241 atctcggtca cccagacctg cgcagaaagc gcgcgcaatg cctgtgcaca gcccttgccg
     3301 acatcgccgt agccagccac cagggccacc ttgccggcga tcatgacgtc ggtcgctcgt
     3361 ttgatgccat ccaccagcga ctcgcggcag ccgtacagat tgtcgaactt gctcttggtg
     3421 accgaatcgt tgacgttgat tgcgggaaag gccagctgcc cctcctttgc catctgatag
     3481 aggcggtgta ctccggtcgt ggtctcttcg gtcacgcctt tcacgtgtcc cagccgcgtg
     3541 gagtaccact tcggatcact tcgcagcctg ccctgaatgg ctgcgaacaa tgcgctctcc
     3601 tcatcgctgg agggcttgct gatcaggctg gcgtcgcgct ccgcacgcgc tccgaggtgc
     3661 agcaacagtg tcgcatcgcc accgtcgtcc aggatcatgt tggagaagcc gccgtcggtc
     3721 cattcgaaga tccggtgtgt gtattcccag taatccgcca gtgattcgcc tttccaggcg
     3781 aacaccgggg tgccccgggc cgcaatggcg gcagccgcgt gatcctgggt cgagaaaatg
     3841 ttgcaggatg cccagcgcac ctgggcaccg agggcctgca acgtctcgat caacaccgcg
     3901 gtctggatgg tcatgtgcag agagccgctg atgcgggcgc cgcgcagcgg ctgcagcggc
     3961 gcgaactcct cgcggatcgc gatgagcccg ggcatctcgg tctcggcgat caggatctcc
     4021 ttgcgccccc agtctgcgag agctaggtcg gcgacgtgat ggtcaggatt gcgcgctgcc
     4081 tgtttgagat tggcgttcat gctcgaatac tccgataaag tacgagcgcc gtgttgatca
     4141 atcgcgacgc caccgagcct ggcagactcg gcacgttcac tcccgtgcca gtccgtcgca
     4201 gcgctcctcg gcagcgcacg ctttgccgca cctgtgcggc ttgattctgt tatgcggctc
     4261 gggcagctcc cttggcctcg tcagcgagct ccgcagcttt gtcggtcctt tcccaggaaa
     4321 actccgcctc ggtacgtccg aagtggccgt aagcggcagt cttctgatag atcggccgga
     4381 ccaggtccag catctcgcgc aatccgtagg gcgtgagatc gaaatgcttg cgcacgagct
     4441 gcgtcaggcg ctcgcgcgac agccggctcg tcccaaaggt atcgaccatg atggaagtag
     4501 gctcggccac accgatcgcg tacgacacct ggacctcaca acgctccgcg agtccggccg
     4561 ccacgatgtt cttggccacg tagcgggccg cataggccgc cgagcggtcg accttcgagg
     4621 ggtcctttcc ggagaaagcg ccgcccccat ggcgtgcgta gccgccatag gtgtccacga
     4681 tgatcttgcg cccggtcagg ccgcagtctc cgaccgggcc gccgatgacg aaccggcctg
     4741 tcgggttgat gtgatacttc gtgcgcttgt ccagccactt ggcgggcaag ctcggcttga
     4801 ggatctcctc catgacggcc tcgcgcagtg ccttggtgga cacctccggg ttgtgctgtg
     4861 tggacagcac caccgcttcg attccgaccg ggtggtcatc ctcgtaacgc agcgtcacct
     4921 ggcttttcgc atcgggacgc aaccaggcga gcttgccgga cttgcgcacc tgcgcctgcc
     4981 gcttgaccag gcggtgtgcc agggtgatcg gcgccggcat gagcacatcg gtctcattgg
     5041 tggcgtaacc gaacatcagc ccctgatcgc ccgcgccctg cttgcgctcg tccttgcgat
     5101 cgaccccctg cgcgatgtcg ggggactgct tgccgatgat gttgacgacg ccgcagctcg
     5161 ccccatcgaa gcccatctcg gaggagttgt agccgatctc gagcaccgtc ttgcgcacca
     5221 gtgcttcgac atctacccag gctttggtcg tcacctcgcc ggcgaccacc acgacgccgg
     5281 tcttgatcag cgtctcgcag gcgacccggc cccgctcatc ctccctgaga atcgcatcca
     5341 gcatcgcatc ggagatctgg tcggcgacct tgtccgggtg cccttcggag acggattcgg
     5401 aggtgaagag ataggtgttg gccatttgaa ttaatccgca ggactgcttc agtgaggaac
     5461 gaaccgccag tctaccgcac tgccgggtta tggcgcggca gcccggtgcg cttcctgcgg
     5521 ccactcaggg acttgcaacg cccccaaagc tctgctcgag gcgctgcttg agctccgctg
     5581 aggtgaagcg gtggttctgg gtgccgaggt attcgatctt gatggccccc agcaccgagg
     5641 cgatctggcc ggcccttcgc aggtcaagcc catgcatcag cccgtggatg atcccggcac
     5701 gataggcatc tccacatccg gtcggatcca ccacagcctt cgccttcacg caggcgatct
     5761 cgatctgctc ccgctcggtg tagatcacgg acccctgagc tcctcgcgtg acgatcaagg
     5821 cctgcactcg acgggtcaca tccttcacgc tccagccggt cttctgctgc acgagctgcc
     5881 actcgtaatc attcatggcc acccagctcg agcgctcgat gaatcgcctc agctcctcgc
     5941 cgtcaaacag cggcaacccc tgtcccggat cgaagatgaa aggaatgcgg gccgcggcga
     6001 actgctcggc atgctcgagc atgccctggc ggccatccgg ggccacgatg cccatgccga
     6061 tctcggctgc atccgggacg cggttcagat gggactgctg catggcaccg gggtgaaacg
     6121 ccgtgatctg gttgtcgtcg agatcggtcg tgatgaatgc ctgcgcggtg tgctccgatt
     6181 cgaggacctt cacgtaatcg gccggaacgc ccacacgcgc catccactcg cgatagggac
     6241 cgaagtcgcg ccccaccgtg gccatcgggt aggccacatc gccgagcagc ttgaggttat
     6301 aggcgatatt gccggcacag ccgccaaatt cccggcgcag ttgcggcacc agaaacgaca
     6361 cgttcaggat gtggatcttc tcgggcagga tgtgacggct gaaatgatcc tcgaacacca
     6421 tgatcgtgtc gaaggccacc gagccgcaga tcagaacgcg gcggcgggag ggttcgatcg
     6481 ttggatgggt catcggctcg actcgctcct gggtttgcgg ctcacgacaa aggtttcgtc
     6541 attgaaccag agaccggaat acttctgcag caccgcctgc gtcgccgcca ggtaggcgtt
     6601 ggattgaacg gccgcatcca ggcgctcgtc ctcgatctga ttgacgtaga tcgccgcgtt
     6661 ccaggctgca aacaacgtcg acgtgccgat ccgatccccg atttccgacg ggagcgtatg
     6721 catctcgtaa cggaacagcg acgcctggtc ctcggggatg ctgagatcgt agtccaccgc
     6781 ggactctccc agctctgccg cgagggccgc gagcagctcg cgccgagcca ccttgaaagg
     6841 gtcctctccc ggccacagcc tgtggatgat ctcgagtccc ggatcatgac cataggactg
     6901 gatcgccaga agtcggccac ccgggacgag gctacgggcc agcggagcca gcactttacg
     6961 tgccttgaac tgcgccggca tacgcgcccg ccacggctgc gaggcgagca ccaggtcgta
     7021 tcccggcagt acctgcctcg cctgcggaat caccccgtcg agcaggaatt cgtggtcggc
     7081 gcgatggagc acgagcactg atgggcgcac gtacaccgga ttgcccgact gcagactcgg
     7141 acgagtctgc cagccttctg ccagcgtcgg cccgagctcc tcgatctgcg cggcatattc
     7201 gtcggccgaa gtgcccgcga gtgcgatctt ctgccagttc agcgcggcgg catcggtctg
     7261 ctcgcgcggc ctgagctgcg gggcatccgc atagttgaga ttggtgatca cgagtacggt
     7321 ggccggatgc tcgtagaaac gatcgggcat cttttccagc cccagcctga catcctcgag
     7381 actgatctcc ttggcgacca cgagcagcgg cacggtcgga tagacaaaat gtgtgctgcg
     7441 catcaggcgt gccaacaccg ttgcatcgcc catgcccgca tcgaagacgc gtagcgccgg
     7501 aggcgtcggg cgcagaagcc gtagctcgcg ggccgcacgt cgcgcgatcg cggccttctc
     7561 attgcaggtg ttgacgaagg cgagatactt ctggcggttg tcgtagaagc gaaacggggt
     7621 cggtccgaaa ccagttggac ctccagcgga agtgggcaaa cccttctgcg agctcatccg
     7681 ccggcgcgcc tccggcaact catttcaagc gcttcggatg ggaatagacg acatgctgct
     7741 cgcggcgcgc aaacgcgacg agcgtcaggc ccgatttttc ggccagacgc accgcaaagg
     7801 cggtgggtgc ggagaaagcc gcgaggaacg ccactccggc tgcggcgctc ttctgaacca
     7861 tctcgaaact cgcgcgactg gtcaccagca tgtagcccga gccgaagtcg atcccgccgc
     7921 gcacgagcgc gccgatcgcc ttgtcgagcg cattgtgacg gccgacgtct tcgcgcacga
     7981 gctggatgcc tttgcccgga atgacccagg cggcggcatg aacgctgccc gtgcgagagt
     8041 tgataggctg catcgtgcca agctgatcga tggccgcgtg caggtcctcg accgtcacgc
     8101 tgggacccgc tcccaccggg gcaatctcgc gaatggcatc ctcggccttc tccgcgccgc
     8161 acagaccgca gccggtgcgc cctgtgagat tgcggcggcg ttgcagcagc gcggagaacc
     8221 ggtcccacgc cacggtgatg cgcacatcga tggcctcttc gccctgagtg acctccaccg
     8281 atcggatttc ctcgggccgc gcgacgagtc cctcgctcaa cgtgaagccg accgcgaagt
     8341 cctcgaggtt cgccggagtg gccagcatca cgacgtgcgg cacaccgtga tagacgaggg
     8401 cgatggggat ttcctccgcc acctgatcgg tcttgtgcgt caccgcgccg cgaacccagc
     8461 ggtcgacggt gac
//
LOCUS       DASRHN010000049         8361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1172587, whole genome shotgun sequence.
ACCESSION   DASRHN010000049 DASRHN010000000
VERSION     DASRHN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172587"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFB37_01855"
     CDS             <1..246
                     /locus_tag="VFB37_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZO21218.1"
                     /translation="EFAGVRVHVLHDLKELDLDPVAEGFRIVVAGHSHRPAILEREGV
                     LFVNPGSAGPRRFRLPVALGFLEISSKSPRARIQPLL"
     gene            576..1910
                     /gene="rimO"
                     /locus_tag="VFB37_01860"
     CDS             576..1910
                     /gene="rimO"
                     /locus_tag="VFB37_01860"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZO21219.1"
                     /translation="MSTDIKQRVPKVGFVSLGCPKALVDSERILTRLRAEGYAISPTY
                     EGADVVVVNTCGFIDAAVDESLEAIGEALEHNGRVIVTGCLGARPERILERHPRVLKV
                     TGPHAYEDVVGAVHEHLPPQHDPFLDIVPAQGIRLTPRHYAYLKISEGCNNKCSFCII
                     PSMRGRLVSRRIDDVLREAERLVTAGVRELLVISQDTSAYGADLRYSPASWRGDSYQT
                     RFIDLAKGLGTLDAWIRLHYVYPYPHVDEVIPLMAARRVLPYLDIPFQHGSPTVLKRM
                     RRPAHGQDTLQRIRTWRRECPDLVLRSTFIVGFPGETEAEFEELLQWLEQAQLDRVGC
                     FKYSPVQGAAANALADPVPPAVQEERYGRFMERAAAISAERLSARVGRNMRVLVDSIE
                     GTTAIARSEADAPEIDGVVKIANARKLRAGDWADVKITSSDAYDLHARLIPV"
     gene            2147..4252
                     /locus_tag="VFB37_01865"
     CDS             2147..4252
                     /locus_tag="VFB37_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO21220.1"
                     /translation="MTHPQRAIFVAIIGIYLGALAYADADDPYLWLEDAHSPKAMAWV
                     EAENAKTVSVLEKDPRYSGLYQDALTLAQAKDRIPTPNILAGAVYNFWQDAEHVRGIW
                     RKTTLESYRTSNPQWSTVLDLDALAAAEKANWFLTDTVCAEPSERRCLLSLSDGGEDA
                     VTVREFDLTSDSFPAKGFVLAKGKQRVAWENQETLLVSREWAPGELTASGYPFIVERL
                     KRGQPLTAAKEIFRGTANDGGYGVTPQVLRDAAGNQAILITRPVSTFESENYLVTGAR
                     VARLALPKKLEIQGLIDGKIVVSLAEDWLVDGKTLTSGSLVAFDLKAAARDPEHLKTE
                     LLYVPGPRESFSESSTTRDALVVATFDNVRGRAWAYRRNADGSWAHMPLELPENATIR
                     VTDTSFHSDHAFLSVAGFLQPSGLWELDTKAHALAEIKTLPPKFDASKEAVEQFEAAS
                     SDGTRIPYFVVHPKDMALDGTNPTILYAYGGFQISMTPSYSASMGKLWLEKHGVFVLA
                     NIRGGGEFGPAWHEAGLKTHRQIIYDDFAAVARDLIARKITSPRRLGIEGGSNGGLLM
                     GVEFTQHPELWNAVDIQVPLLDMVRFEKIAAGTSWVGEYGSVSNPDEAAFLTRISPYA
                     NIHRGTHYPEPFIWTTTKDDRVGPQHARKFAARLSEYGIPYLFYEVVEGGHGSGANLA
                     EKAHTTALEMTYFTRKLMD"
     gene            complement(4289..6007)
                     /locus_tag="VFB37_01870"
     CDS             complement(4289..6007)
                     /locus_tag="VFB37_01870"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZO21221.1"
                     /translation="MKQRILITALAGLCMTTAQADSTGAAGPLYDLIIRNGHVIDGTG
                     SPWYSADVAIREGRIAAIGRLHGATAKKTIDAQGLAVTPGFIDMLGQSELTMLVEPHV
                     PSKIFQGITTEITGEGNSVAPLNERMIAADRVTYEHLHIDPNWRTLAEYFHRLEHQGL
                     GINLATYVGATSVRRMVIGDDDRAPTAQELARMKDLVGESMRAGAFGLSSALEYTPAP
                     YAKTDELIALAAEAGRYGGIYATHMRSEQSGIMEALDEAIRIGREAHVPVEIWHLKTG
                     SKAIWGRMKEVVAKIDNARTSGVDVTADTYAYTAWFNTFSAFVPPWAHDGGDAKLIER
                     MKDPATRARIRRDILSSEGSWDNEWQAIPGPEAILICVVQNPALRALQGKTLAQAAAM
                     RHEDPIDALLNILIEDKAFTSVAVFGMREDDVALALSQPWVAVDNDSEGTAPTGLLGS
                     EHPHPRAYGTFPRILRKYVREDHLLSLADAIRKFTSLPAQRMGLTDRGVLKQGMWADI
                     DVFDPANIRDLATYENPNQLSVGMRYVFVNGVAVIAEGKGTNALPGHVLRGPGYLVAT
                     HEAAKE"
     gene            6097..7518
                     /locus_tag="VFB37_01875"
     CDS             6097..7518
                     /locus_tag="VFB37_01875"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HZO21222.1"
                     /translation="MSAEPEAVHHLFLSLETRVTCPKCTSRFSLQQGFAQQALEKLEQ
                     STEGALEAVRASERAEVSRQAQQLAAQRDKTLRAENSQLQQLLREQGEQQQRLLKAQS
                     EQHAKALQEVRAVAEKSLAPQLEALRGELTASQARVSELRSTEITLRQQKAELADRAA
                     ALEVEVVRRVDAQRTELETRIRTQERERAELEKAELQKRLDDVHGQLAEAQRKSQQTS
                     QQLQGEVLELTLQSGLERAFPLDSVEEVKKGARGGDVVHQVATRVGQVAGVLLWEAKR
                     ARDWSGQWVTKLKEDMRACNADVGILVITGTALPREWEPGQLFGLHEEVWVTSWTTAL
                     QLAAVLRSGLLDLHKQRLVSANKGEKMEAVYDYLTSSQFAQKLKAVYGAFYRMRQELE
                     SEKSQTLQRWARREKQLQCGLAELVGVAGDIQGLAQQDLPLLDIDPGMEADASPGSDG
                     AAGLGAEASQLGLGIGEQKSTGS"
     gene            complement(7559..>8361)
                     /locus_tag="VFB37_01880"
     CDS             complement(7559..>8361)
                     /locus_tag="VFB37_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21223.1"
                     /translation="RTGAEIRVLKHALLVNSAVFSPDGARIATASDDQTARIWDARTG
                     AQLLVLAGHRDGVTAVTFSSDGAYLLTGSMDRTTRLWDATTGTLIAVYVQPGIVSDVA
                     FSPAGHHVLTVSEDGSVRTWSIEDVSALRLQVSWYRAAEFDPLSAIEKAQLGLAENAS
                     AGIHLFHRDDGKDAAALARLAQGEERAATLDLSSRERNAHLLHAFTLYAAAARRSTAD
                     GRPEELSKAWRYRRATLAHVLARQGMMRQAAETYRALVSGTGVSYASR"
BASE COUNT         1527 a         2632 c         2700 g         1502 t
ORIGIN      
        1 gagttcgccg gggtgcgcgt tcacgtgctg catgacctga aggagctcga tctggatccg
       61 gtggcggaag gctttcgcat cgtagtcgcc ggccactcgc accggcccgc aatactcgag
      121 cgggagggcg tgctgttcgt caatcccggc agcgcgggcc cgcgccgatt caggcttccg
      181 gtcgcgcttg gatttctgga gatctcatcc aagtcacccc gggcacggat ccagcccctg
      241 ctctgagcca gaacgctaag cgcccggcag cgctcggccc gccctattgc cggacccggg
      301 gggcaggggt aggctttgcg cgccgtggct gcagtctctc ccaattcact ggtttctggc
      361 ggaccgtccg agcggcggcc ggcgatctgg ccgtggctcg tgatgcccct ggtcacgctg
      421 gccctgttct ttgccctgaa cagactgcac cagagcacag cgcccgcggc ctcattcgag
      481 gctggcccgg acaccgcggc cagcagttcg gcggagtcca ccactccctg actgcgcatc
      541 catccacgcc gcggcgcgcg ccgcgggctc aagacatgtc cactgacatc aagcaacgcg
      601 tcccgaaagt cggtttcgtc agtctggggt gccccaaggc cctggtcgac tccgagcgga
      661 tcctgactcg tcttcgcgcc gagggctacg ccatctcacc cacctacgag ggcgctgacg
      721 tggtcgtggt caatacctgc gggttcatcg atgccgcggt ggacgagtcg ctcgaggcaa
      781 tcggagaagc gctggagcac aacgggcggg tcatcgtgac aggctgcctt ggagcgcgac
      841 cggaacgcat cctcgagcgt catccacgag ttctgaaagt gactgggcca cacgcctacg
      901 aggacgttgt gggggccgtg cacgagcatc tgcctccgca acacgatccg ttcctggaca
      961 tcgttcctgc ccagggcatc cgtctgactc cgcgccatta tgcgtacctg aaaatctcgg
     1021 aaggctgcaa caacaagtgc agcttctgca tcattccctc gatgcgcggc agactggtga
     1081 gtcgccgaat cgacgatgtg ctgcgtgaag cggagcgcct ggtcacggcc ggagtccggg
     1141 agctcctggt catctcgcag gatacgagcg cgtatggcgc cgatctgcgc tacagcccgg
     1201 cctcctggcg cggagattcg tatcagacgc gtttcatcga tctggcaaaa ggcctgggta
     1261 cgctggacgc ctggatccgg ctgcactacg tgtatcccta tccgcacgtg gatgaggtga
     1321 tccccctgat ggcggcgcgg cgcgtgctgc cctatctgga catccccttc cagcacggaa
     1381 gtccgacggt cctcaagagg atgcgccgtc cggcgcatgg gcaggacacc ctgcagcgca
     1441 tccgtacctg gcgcagggaa tgtcccgacc tcgttctgcg cagcacgttc atcgtcggat
     1501 ttccgggcga gaccgaagcc gaattcgagg aactgctgca gtggctggag caagcgcagc
     1561 tcgatcgggt tggatgtttc aagtattcac ccgtgcaggg cgcagcggcg aatgcgctgg
     1621 cggatccagt gccgcccgcc gtgcaggaag agcgctatgg gcgcttcatg gagcgagctg
     1681 cggcgatcag cgcggaacga ctttccgcgc gtgtcggtcg caacatgcgt gtactggtgg
     1741 acagcatcga gggcactacg gcgatcgcgc gcagtgaagc cgacgcgcct gagatcgacg
     1801 gcgtggtgaa gatcgccaac gcccggaagc tgcgggctgg cgactgggca gatgtgaaga
     1861 tcacctcatc cgacgcttac gacctccacg cccggctgat ccccgtctga cattctgggg
     1921 caacttcgag tcagcgcgct ggcgctgatt tcttgacgtc ggttccgaca gaccgtcagt
     1981 gcaatgcgag cgtgtcgcgg cgccaggccg gatcctgcca gcggccgaag tctgccagca
     2041 gctccgcaga gcaggcctgt ctcaccagcg tgagatcatc caaacgatgg cgtgaccggg
     2101 gcgggcgtta tactgggaat cggcacaaat cacggatcgg aaccccatga ctcacccgca
     2161 gcgagcgatc tttgttgcaa tcatcggcat ctacctgggg gcactggcgt atgcggacgc
     2221 cgatgatcct tatctttggc tcgaggacgc tcacagtccc aaggccatgg cgtgggtaga
     2281 ggcggagaat gccaagacgg tgagcgtgct ggagaaggat ccgcgctatt ccgggctcta
     2341 tcaggacgct ttgacgctcg ctcaggcgaa ggatcgcatc ccaactccca atattctggc
     2401 gggagcggtc tacaacttct ggcaggatgc ggaacacgtg cgcggcatct ggcgcaagac
     2461 gactctcgag agctaccgca cttccaaccc gcaatggtcc accgtgctgg acctcgatgc
     2521 actggcagcg gctgaaaaag ccaattggtt cctgaccgat acggtctgcg ccgagccgag
     2581 tgagcgacgc tgcctcctca gcctctcgga cgggggtgag gacgcggtca ccgtccggga
     2641 attcgatctg acgagcgact ccttccccgc aaagggtttc gttctcgcca agggcaagca
     2701 acgtgtggcg tgggagaatc aggagacgtt gctcgtctca agggagtggg cccccggaga
     2761 gttgaccgct tcgggatacc ctttcatcgt cgagcgtctg aagcgtggcc agcccttgac
     2821 cgcggcgaag gagatcttcc gcggcacggc gaatgacggc ggctatggag tgaccccgca
     2881 agtgctgcgc gacgccgcgg gcaatcaagc gatcctcatc acgcggcccg tctccacgtt
     2941 cgagtcggag aactatctcg tcacgggcgc cagggtcgcc aggctggctc tgcccaagaa
     3001 gctggaaatc cagggactga tcgatggcaa gatcgtcgtg agtcttgccg aggactggtt
     3061 ggtcgacggc aagactctga ccagcggctc cctcgtggcg ttcgatctga aggccgccgc
     3121 acgcgacccc gagcacctga agaccgagtt gctctatgtt ccgggcccgc gtgagagctt
     3181 cagtgaatcg agtaccaccc gggatgcact cgtcgtggcg accttcgaca atgtccgcgg
     3241 tcgtgcctgg gcctatcggc gcaacgcgga tggatcgtgg gcacacatgc cgctcgagct
     3301 tcccgagaac gccacgattc gcgtgaccga cacgagcttt cacagcgatc acgcgttcct
     3361 cagtgtggcc ggcttcctgc agccttccgg cttgtgggag ctcgatacca aggctcatgc
     3421 gctggcagag atcaagacac tgccaccgaa gtttgatgct tcgaaggagg ctgtcgagca
     3481 attcgaggcc gcctcgagcg atgggacccg gattccctac tttgtcgtgc atcccaagga
     3541 catggctctg gatggcacca atccgacgat cctttacgct tatggaggct ttcaaatctc
     3601 gatgacgccc tcctactccg cctccatggg caagttgtgg ctggagaagc atggcgtgtt
     3661 cgtgctggcc aacatccgcg ggggcggaga gtttggtccc gcctggcatg aggccggact
     3721 gaagactcac cgtcagatca tctacgacga ttttgccgcg gtcgcgcgag atctcattgc
     3781 ccgcaagatc acgagtcccc ggcgattggg gatcgagggg ggctcgaacg gagggctcct
     3841 gatgggtgtg gagttcacac agcaccctga gctatggaat gcggtcgaca tccaggtgcc
     3901 gctgctggac atggtccgtt tcgagaagat cgccgcggga acctcctggg tcggcgaata
     3961 cggtagcgtc tccaatccgg acgaagccgc attcctcacg aggatatccc cctacgcgaa
     4021 catccaccgc ggaacacact acccggagcc cttcatctgg accacgacca aggatgatcg
     4081 cgtcgggccg cagcacgccc gcaaatttgc tgcacgcctg tcggagtacg gcattccgta
     4141 tctcttctac gaagtggtgg agggcggcca cggatccgga gccaatctgg ccgagaaagc
     4201 ccacacgacc gcgctggaga tgacctactt cactcgcaag ctgatggatt gatccagcga
     4261 accaggctcg gcgactgcgg ggatcggctc attctttcgc cgcctcgtgt gtggccacga
     4321 gatatccggg accccgcagc acgtgtcccg gcaaggcatt cgtgcccttg ccctcggcga
     4381 tcacggcgac gccgttcacg aatacatagc gcatgccgac cgagagttgg ttgggattct
     4441 cgtaggtcgc gagatcccgg atgttcgccg gatcgaacac gtcgatatcg gcccacatgc
     4501 cctgtttgag cacgccgcga tccgtcaggc ccatgcgctg agcgggaagc gaggtgaact
     4561 tgcgaatcgc atccgcgagg ctgagcagat gatcttcgcg cacatacttg cgcagaatgc
     4621 gcggaaaggt tccgtacgct cgcggatgcg ggtgctctga acccagcagc ccggtcggtg
     4681 cggtgccctc cgaatcattg tccacggcaa cccaaggctg ggagagcgcg agtgccacgt
     4741 cgtcctcgcg catgccaaac acggctacgg atgtgaatgc cttgtcctcg atgaggatat
     4801 tgagaagcgc atcgatcgga tcctcgtgcc gcatcgcggc cgcctgcgca agggtcttac
     4861 cctgcagcgc tcgcaacgcc ggattctgca cgacgcagat gagaatcgcc tcgggaccgg
     4921 ggatagcctg ccattcattg tcccagctcc cctcgctgga gaggatatcc ctacggatcc
     4981 gcgcccgcgt cgccggatcc ttcatgcgct cgatcagctt ggcatccccg ccatcgtgag
     5041 cccagggcgg cacaaatgcc gagaaggtgt tgaaccaggc ggtgtaggca taggtgtcgg
     5101 cggtgacatc cacgccactc gtgcgcgcgt tatcgatctt cgcgacgact tccttcatcc
     5161 tgccccagat cgccttgctg ccggtcttca ggtgccagat ctcgaccggt acgtgtgcct
     5221 cgcgcccgat gcggatggct tcatccagtg cctccatgat tcccgactgc tccgagcgca
     5281 tgtgcgtggc atagattccg ccgtagcggc ctgcctctgc agctagcgcg atcaactcat
     5341 cggtcttggc atagggcgcc ggggtgtact ccaatgcgct cgagagaccg aacgcgccgg
     5401 cgcgcatgct ctctccgacc aggtccttca tccttgcgag ctcctgggcg gtgggcgcgc
     5461 ggtcgtcgtc gccgatcacc attctgcgaa ccgaggtcgc ccccacatag gtcgcgaggt
     5521 tgatgcccag gccctggtgc tcgaggcggt ggaagtattc cgccagggtg cgccagttcg
     5581 gatcgatgtg cagatgctcg taggtcaccc ggtccgccgc gatcatcctc tcattgagcg
     5641 gggccaccga attgccctcg cccgtgatct cggtcgtgat gccctggaag atcttggacg
     5701 gcacgtgcgg ctcgaccagc atggtgagct cggattgacc gagcatgtcg atgaacccgg
     5761 gtgttaccgc aagaccctgc gcatcgatcg tttttttcgc ggtggcccca tgcaggcggc
     5821 cgatcgccgc tatccggccc tcgcggatgg cgacgtccgc ggaataccag ggcgagccgg
     5881 tgccgtcgat gacgtgaccg ttgcgaatga tgaggtcata cagtggccct gcggcgccgg
     5941 tactgtccgc ctgcgcggtc gtcatgcaca gcccggccag tgccgtgatg agaatgcgtt
     6001 gtttcaatcg atcgccaatc ccctgcgagc ctggatcgca gtatgctcca gatcgttatg
     6061 cagtggcgca agtgtggcgc taccgaggac ctaccggtgt ctgccgaacc tgaagccgtc
     6121 catcatctgt ttctctccct cgagacgcgg gtcacctgcc cgaagtgcac gtcgcggttc
     6181 agcctgcagc agggctttgc ccagcaggcg ctcgaaaagc tcgagcagtc caccgaaggt
     6241 gcgctggagg ccgtgcgcgc gagcgagcgc gccgaggtca gtcgccaggc gcagcagctc
     6301 gcagcgcaac gagacaagac cctgcgcgcc gaaaacagcc agctgcaaca gcttttgcgc
     6361 gagcagggcg agcagcagca gcgcctcctc aaggcgcaat ccgagcaaca cgccaaagcg
     6421 ctgcaggaag tgcgtgccgt tgccgagaaa tcgctcgctc cgcagctcga ggctctccgt
     6481 ggagagctca ccgcgagcca ggccagagtc tcggagctgc gctcgaccga gatcactctg
     6541 cgccagcaaa aggccgagct cgcagaccgg gcggccgctc tggaagtgga ggtggtgcgc
     6601 cgggtggacg cgcagcgcac cgagctcgag acccgcatcc gcacgcagga gcgcgagcga
     6661 gcggagcttg aaaaggccga gctgcagaag cggctcgatg atgtgcacgg ccagctggcg
     6721 gaggcgcagc gcaagagtca acagacctcg cagcaactcc agggtgaggt cctcgaattg
     6781 accctgcaga gcggtctcga gcgggccttt ccgcttgact ccgtcgagga ggtcaagaag
     6841 ggcgcccgcg gcggcgacgt cgttcaccag gtcgcgacac gtgtgggaca ggttgcgggc
     6901 gtgctgctct gggaggccaa gcgcgcccgg gactggagcg gccaatgggt gacgaagttg
     6961 aaggaagaca tgcgcgcctg caatgcggat gtcggaatcc tcgtgatcac gggtaccgcg
     7021 ctgcccaggg agtgggagcc ggggcaactg ttcggcctgc acgaggaggt ctgggtcact
     7081 tcctggacca cggcgctgca actcgccgcc gtcctgcgct caggattgct cgatctgcac
     7141 aagcagcgcc tggtctccgc caataaaggc gaaaagatgg aggcggtgta cgactatctg
     7201 acctcttccc agttcgccca gaagctcaag gcggtttatg gcgccttcta ccgcatgcgt
     7261 caggagctcg agagcgagaa gagccagacc ctgcagcgtt gggcacgtcg cgagaagcag
     7321 ctacagtgcg gcctcgccga gctcgtcggc gtggcagggg acattcaggg gcttgcgcaa
     7381 caggatctgc cgctgctgga catcgatccc ggcatggaag cggatgccag tcctgggagc
     7441 gacggtgccg cgggcctcgg ggccgaggcg tcgcagctcg ggctgggtat tggcgagcaa
     7501 aaatcgactg ggagctagtc acccgtcctg gacacgcgat ctctctcgag tgctggcatc
     7561 agcggctcgc gtacgaaacg cctgttccag agacgagggc tcgataggtc tcggcagctt
     7621 ggcgcatcat cccctgccgt gcaagcacgt gtgccagggt cgcgcgtcga tatcgccatg
     7681 ccttcgagag ctcctccggg cggccgtccg cagttgagcg cctggcggcg gccgcataga
     7741 gcgtgaaagc atgaagcaga tgagcgtttc tttcacgaga gctcaggtcc aaggtggcag
     7801 cgcgctcttc accttgggcc aggcgggcga gggccgccgc gtccttgcca tcatcacggt
     7861 gaaagagatg gatgccggcg ctcgcattct ccgccaggcc aagttgcgcc ttctcgatgg
     7921 ctgacaaggg atcgaactcg gctgcacggt accaggaaac ctgcaggcgc agtgctgaga
     7981 cgtcttcgat actccacgtt cgtaccgatc catcctcgga cacggtgagt acgtggtggc
     8041 cagcggggga gaatgccaca tcgctcacga tccccggctg cacgtaaaca gcaatcagag
     8101 tgccggtggt ggcgtcccaa agccgggtgg tgcggtccat tgaccctgtg agcagatagg
     8161 cgccgtctga agagaaggtc accgcagtga ccccgtcgcg atgccctgca aggaccagca
     8221 actgggcacc agtgcgtgca tcccagatgc gcgctgtctg gtcatcggaa gcggtcgcga
     8281 ttcgcgctcc gtcaggtgag aacacggcgc tgttgacgag cagagcgtgc ttgagcacgc
     8341 ggatctcagc cccggtccgc g
//
LOCUS       DASRHN010000050         8380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_843583, whole genome shotgun sequence.
ACCESSION   DASRHN010000050 DASRHN010000000
VERSION     DASRHN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843583"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      42..128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            462..1526
                     /locus_tag="VFB37_01885"
     CDS             462..1526
                     /locus_tag="VFB37_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZO21224.1"
                     /translation="MFLFQRKYRPLLGLDITTSSVKLIELAMAGGQYRVEAYAAEPTP
                     QNAINEKAIVDAEAVGEAIRRAVKRAGAKSGEVAIAISGDAAITKVIQMPRSLRTNDL
                     EAQVEMQADQYIPFPMDEVSYDFEVLGPSEKDSETNDVLLVATRTENVDQRQAAVKAA
                     GLSARIVDVEAFALENACKLMTHQMPDGGIDRSIAVVDFGASSTTFSVLRNLKVVYTR
                     DFAFGGQQLTEEVMRTYGLSMEEAGRAKKEGGLPGNYQSEVLDPFIDDMTQQVSRSLQ
                     FYLASGSGREQPEKIIVCGGCANIPGVADVISSRVGIQAEKGDPLGQMKLSSRAKAQA
                     VQRDATALLTACGLALRSFD"
     gene            1528..2154
                     /locus_tag="VFB37_01890"
     CDS             1528..2154
                     /locus_tag="VFB37_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZO21225.1"
                     /translation="MPRINLLPWREEERKERKVKFTVALGAAALAACFTTFAIYLMYG
                     SMIDAQEHRNEKLRVEIKTLDKQIEEINDLESAKQKFIARMEIIEKLQRSRPEIVHVF
                     DEIVRTLPDGVYLTSVKQTDKRLKFEGVAQSSTRVSSFMRNIDGSEWLRNPELEVVQT
                     SKDNKGPPGSNFTLYADQVSTASQDDASAVKSARKPGAKVMASRGSAQ"
     gene            2151..2816
                     /locus_tag="VFB37_01895"
     CDS             2151..2816
                     /locus_tag="VFB37_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZO21226.1"
                     /translation="MNLMEELRSLDPRDPGRWPFPIRAGAVAACFVVLSIVLSYVFVW
                     NDQRPELQRRADTEGTLRQEFKTKHAKAVNLEVYKQQLKDIERSFGALLRQLPGKTEV
                     PNLLVDISQTGLSAGLEEKLFQPQSEQKRDFYAELPIKIVLTGSYHQFGEFVSGIAAL
                     PRIVTLHDIEIKSVNKDAYDQLTLALTAKTYRYLDEDEVAAAEAEKRKDQQQHHPRPG
                     SGG"
     gene            2841..3359
                     /locus_tag="VFB37_01900"
     CDS             2841..3359
                     /locus_tag="VFB37_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HZO21227.1"
                     /translation="MNRYRGQLVFLMALASTSLGFLSACSSADDELQRFIEDTKKEPG
                     GRVEPLPEVKPYETFVYAAAEMRSPFLPGGSGPGAQGLRPDSKRNREFLEQFSLDTLK
                     MVGTLKLGGRMYGLVQTKDGLVHRVTDGNYMGQADGKITDITPAKIVLTEIVPDGLGG
                     YMERPAALGLNE"
     gene            3403..5559
                     /locus_tag="VFB37_01905"
     CDS             3403..5559
                     /locus_tag="VFB37_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HZO21228.1"
                     /translation="MKAHSPRVQVLATAAWLMIAGILGILPSQAVRAEDGPKLEAIDV
                     QPLPGQQVQLTMRLSGPAPQPLSFTIDNPARISLDLPNTTLALPSRRIDVHSSGLDSI
                     LAAEAKDRTRLVLNLDKLVPYETRVDGNNIIVMLGAGNQAVANTGGATPVLPTRTASA
                     AVGQRAIRSIDFRRSTDGAGRVMVKLTDPHTHINLRQVGSQIVVDLDADLPKNLARHY
                     DATDFGTPVSSFEVTRVGTGTRIAINASGDYEQLAYQSDDEYVVEIQPQRKAAAAQEE
                     KPQYTGERLTLNFQDIETRAVLQLLADASGQNIVVSDSVKGNVTLRLQNVPWDQALDI
                     VLRTKGLDKRKQDNVIIVAPQEELATREKAELAARKDIQELSPLRADYLQVNYAKAAD
                     MAALIKSQGGGSLLSARGNVAVDERTNTLLLQDTADRLADIRRLVASLDIPVRQVLIE
                     ARVVIVNDDFNRQLGARFGYTTAQANGNGIWTTSGTAASTDTAIGSALANTAANGSPF
                     PVQVPTGSTAANRYNVNLPVSSPAGSFALGILGSNYIVDLELSAAQAETNAQIISSPR
                     VITANQKEATIEQGTEIPYQQSASSGATTISFKKAVLSLKVTPQITPDNRIILDIDVK
                     NDSIGTITVASGGVNVPSIDTKEIVTQVLLNDGQTVVLGGVLQTTHREDSTKVPFLGD
                     IPVLGNLFKTTSKTNNKDELLIFVTPKIVREGVNVY"
     gene            5604..7403
                     /locus_tag="VFB37_01910"
     CDS             5604..7403
                     /locus_tag="VFB37_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21229.1"
                     /translation="MHKSFSIPAAAILALALSACGGGENLSGGSSSGTSGTGTGTGTG
                     TGTGTTTTYSMGNGSGSSFQTGVISLSSTSLSAGGTASLTVTIVDQTGTLYTGAAVTV
                     TFNSPCVAQGLATITASGASTAGSSPGTVQTSTGSASATYTAKGCSGSDVITASALVG
                     GQNLSATGTVSVAAASIGSIQFVSATPSTIGLKGTGLNETSTVIFKVTDSTGGDRPGV
                     TVTFALDSTVGGLTISPTSATSAADGTVQTVVSSGTAHSTVRVKASIASPALSTESSA
                     LTVSTGLPSSNAFSISVGPATYGTGASTLACSNVEAYDIDGVTVPVTVRLADRYNNPA
                     PDGTAVAFTTDGGHIVASCTTPSSPTSPGDGTCTALWTSADPRPTTSSVPPAANNGRA
                     MVMATAIGEEFFNDANGNGYYDPGETFANLGEPFRDDNENGTYDHGEYFLDFNKNGVR
                     DPGDGTFKGITCTASSCTTNTLAISASHLIIMSTSAAAVTFVGATGFTGNGSGLSITH
                     STSGTVTIGVADSNGNAMAAGTKVAVSADTGAGTVTQTPSPFIVGCDAGRGGEQVSFS
                     VAAASSAGGGTITIQVTAPNGTNSVFTIPVTVN"
     gene            7475..8002
                     /gene="aroK"
                     /locus_tag="VFB37_01915"
     CDS             7475..8002
                     /gene="aroK"
                     /locus_tag="VFB37_01915"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HZO21230.1"
                     /translation="MLGKPNLFLIGPMGSGKSAVGKHLSRVLNVPFHDSDAEIERRTG
                     VDIPFIFEKEGEAGFRARERDVIEALTALEPIVLATGGGAVLLPENRKVLAERGRVVY
                     LETSVAQQADRVRQGRARPLLTNVDRQAKLEELMDKRGTLYAQIADLTVATDSRKVHY
                     VAELILRELDSRARG"
     gene            8117..>8380
                     /locus_tag="VFB37_01920"
     CDS             8117..>8380
                     /locus_tag="VFB37_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZO21231.1"
                     /translation="MDTLKVELGERSYPILIGAGLLQEPDRIREHIDARDVLIVSNSA
                     VGPLYVPPVTAGLSAQRVLEAILPDGEAHKTLTTVTRILDVLVA"
BASE COUNT         1626 a         2709 c         2631 g         1414 t
ORIGIN      
        1 ctcagcttgc gctactaact tcggctatgc atagtaacgc ggtgatctaa gggcaaactc
       61 accgaaaggt ggggacgcaa agcctccggt ctacgggacg aagtgcctat gacagcgggg
      121 ttgccggcgc gaacgttaac ctactccgcg tacgctgacc aagcgtacgt cgcgatttac
      181 ggttcggccg aacctcgcca tgcgcccctt cgccgtagtt cacggaattc gtgcgccccc
      241 ccgagcgata gttagacatg gcacagcaca aatcgttgac cctggccgcg ggtctctcca
      301 ggcgagagcg ccgtagcaat tcccgcagcc tgaacgggtc gggaccgggt ttgtgtgagc
      361 gggctcacgt tctatttaac attgcgctga tatatagttg cctccgcttt gacgcggggt
      421 cagcgttttc aggcgtaagt tcctgaaaag gaaataaaat aatgttcctc ttccagcgca
      481 agtaccgtcc gctgctgggg ctcgacatca ccacctcgtc ggtgaagctg atcgagctgg
      541 ccatggcggg gggtcagtat cgggtcgagg cctatgcggc cgagcccacg ccccagaacg
      601 ccatcaacga gaaggcgatc gtggacgccg aggccgtcgg cgaggccatc cgccgggccg
      661 tgaagcgggc cggtgccaag agcggtgagg tcgcgatcgc gatcagcggc gatgccgcga
      721 tcaccaaagt catccagatg ccacgctcgc tgcgcaccaa cgatctcgag gcgcaggtgg
      781 agatgcaagc cgaccagtac attccatttc cgatggatga ggtcagctat gacttcgagg
      841 tgctcggtcc ttcggagaag gattccgaga ccaacgacgt tctgctggtt gccacgcgca
      901 ccgagaacgt cgatcagcga caggccgcgg tgaaagctgc cggactgagc gcccggatcg
      961 tggacgtcga ggcgtttgcc ctcgagaacg cctgcaagct gatgacacat cagatgcccg
     1021 acggtggtat cgatcgttcg atcgccgtag tggatttcgg agcgagcagc acgacattca
     1081 gcgtgctgcg caatctcaag gtcgtgtata cgcgcgattt cgccttcggc gggcagcagc
     1141 tcaccgagga agtcatgcgc acctacggcc tctcgatgga ggaagcgggc cgtgcaaaga
     1201 aggaaggcgg cctgccgggc aactaccagt ccgaggtgct cgatccgttc atcgatgaca
     1261 tgactcaaca ggtcagtcgc tcactgcagt tctatctcgc ttccggctcg ggccgcgagc
     1321 agcccgagaa gatcatcgtg tgcggcggtt gcgccaacat tccgggagta gccgacgtga
     1381 tctccagccg cgtgggcatc caggcggaaa agggcgatcc actcggacag atgaaactgt
     1441 cgtcccgcgc caaggcacag gccgtacaac gggacgccac ggcgctgctc acggcctgtg
     1501 gtctcgcact gaggagcttc gactgacatg ccgcgcatca acctacttcc ctggcgcgaa
     1561 gaagagcgca aggagcgcaa ggtcaagttc acggtggctc tcggagccgc cgccctggcc
     1621 gcctgcttca cgaccttcgc gatctacctg atgtacgggt ccatgatcga tgcgcaggag
     1681 caccgcaacg agaagctgcg cgtggagatc aagacgctcg acaagcagat cgaggagatc
     1741 aacgacctcg aaagcgcgaa gcagaagttc atcgcacgca tggagatcat cgagaagctc
     1801 cagcgctctc gcccggagat cgtgcacgtg ttcgatgaga tcgtgcgtac gctgcccgac
     1861 ggggtctacc tgacctccgt caagcagacc gacaagcggc tgaagttcga gggcgtggcg
     1921 caatcgagca cgcgcgtgtc ctccttcatg cgcaacatcg acggctcgga gtggctgcgc
     1981 aatccggagc tggaggtcgt tcagaccagc aaggacaaca aggggccccc gggctccaat
     2041 ttcactctat acgccgacca ggtctccact gcctcccagg atgatgcctc ggcagtgaag
     2101 agcgcgcgca aaccgggcgc caaggtcatg gccagcaggg ggagcgcgca atgaatctga
     2161 tggaggaact gcgatcactg gatccgcgtg atccggggcg ctggcctttc cccatacgag
     2221 caggcgcagt cgcggcctgc ttcgtcgtgc tgtcgatcgt gctctcctac gtgttcgtct
     2281 ggaacgatca gcgtccggag ctgcagcggc gtgcggatac cgaaggcacg ctgcgccagg
     2341 agttcaagac caagcacgcc aaggcggtca acctcgaggt ctacaagcag cagctcaagg
     2401 acatcgagcg ctccttcggc gcgctgctgc gccagcttcc cggcaagacc gaggtgccga
     2461 acctgctggt ggacatctcg cagaccggac tttcggccgg tctggaagag aagctgttcc
     2521 agccgcagtc ggagcagaag agagacttct atgccgagct gcccatcaag atcgtgctga
     2581 ccggcagcta tcaccagttc ggcgagttcg tgagcggcat tgcggccctg ccccgtatcg
     2641 tcacgctgca cgacatcgag atcaaatcgg tcaacaagga cgcatacgac cagctcacgt
     2701 tggccctgac ggcgaagacc tatcgttatc tcgatgagga cgaggtcgcc gccgccgagg
     2761 ccgagaagcg caaggatcag cagcagcatc atcccagacc cggtagtgga ggttaggccc
     2821 cctgaccggg gttggtcact atgaacagat atcgtggtca gctagttttc ctgatggcgc
     2881 tggcgagcac ctcgctcggc ttcctgtcgg cctgctcgag cgccgacgat gagctgcagc
     2941 gcttcatcga ggatacgaag aaggagccgg gcggacgcgt cgagccgttg ccggaggtca
     3001 agccgtacga gaccttcgtt tacgcggctg ctgagatgcg ctctcccttc ctgcctggcg
     3061 gcagtggccc gggcgcgcag ggactgcggc ccgactccaa gcgcaatcgg gagttcctcg
     3121 aacagttctc gctcgatact ttgaagatgg tgggcacgct gaagctcggc ggccggatgt
     3181 acgggctcgt ccagaccaag gacggcctgg tgcaccgcgt gaccgacggc aactacatgg
     3241 gccaggcgga cggcaagatc accgacatca cgccggcgaa gatcgttctc accgaaatcg
     3301 tgccggacgg cctcggcgga tacatggagc gccccgcggc gctcggtctc aacgaataag
     3361 tctccgactc agtcgcacag gttcttcagg gagttcgcac gaatgaaagc tcacagcccg
     3421 agagtacagg tcctggccac ggcggcgtgg ctcatgatcg ccggcattct cgggattctc
     3481 ccgagtcagg cggtccgcgc cgaggacggc cccaagctgg aagccatcga cgtgcaaccg
     3541 ctgcccggcc agcaggtgca gttgaccatg cgcctgtccg gaccggcccc tcagccgctg
     3601 tcgttcacca tcgacaatcc ggcccgcatc tcgctcgacc tgcccaacac gacgcttgcg
     3661 ctgccctcgc gccgcatcga cgtgcactcc tcgggccttg attccatcct ggccgccgaa
     3721 gccaaggacc gtacccggct ggttctgaac ctcgacaagc tcgtgcccta cgagacgcgt
     3781 gtcgatggca acaacatcat cgtcatgctg ggtgccggca accaggctgt cgccaacacg
     3841 gggggcgcca ccccagtcct gccgacccgg accgcctccg cagccgtcgg gcagcgcgcg
     3901 atccgcagca tcgacttccg ccgcagcacc gatggtgcgg gccgcgtgat ggtcaagctg
     3961 acggaccccc acacacacat caacctgcgc caggtcggca gccagatcgt ggtggacctg
     4021 gatgcggatc tgcccaagaa cctggcccgg cattatgacg ccaccgactt cggcactccg
     4081 gtcagctcgt tcgaggtcac gcgcgtgggc accggtaccc gcatcgcgat caatgcctcg
     4141 ggtgactacg agcagctcgc ttaccagtcc gacgatgagt acgtcgtgga gattcagccg
     4201 cagcgcaagg ccgctgctgc acaggaagag aagccgcagt acaccggcga gcgcctcacc
     4261 ctcaactttc aggacatcga gacgcgtgcg gttctgcagc tgctcgctga tgcgagcggc
     4321 cagaacatcg tggtcagcga ctcggtgaag ggcaatgtga cgctgcgact gcagaacgtg
     4381 ccctgggatc aggcgctcga catcgtgctg cgtaccaagg gactggacaa gcgcaagcag
     4441 gacaacgtga tcatcgtggc cccgcaggaa gagctcgcca cgcgtgagaa ggcagagctg
     4501 gccgcccgca aggacatcca ggagctctcg ccgctgcgcg ccgactacct gcaggtgaat
     4561 tacgccaagg ccgctgacat ggcggcgctg atcaagtcgc agggcggcgg ctctctgctc
     4621 tctgcccgcg gcaacgtcgc cgtggacgag cgcaccaaca cgctgctcct acaggatacg
     4681 gccgatcgct tggccgacat ccgccgcctg gtggcatcac tcgacatccc ggtgcgccag
     4741 gtgctgatcg aggcgcgcgt cgtcatcgtc aacgacgact tcaatcgcca gctcggcgcc
     4801 cgcttcggtt acacgacggc gcaggcgaac ggcaatggca tctggacgac cagtggaacg
     4861 gccgcctcca ccgacacggc catcggctcc gcgctcgcca acacggcagc caacggcagc
     4921 ccgttccccg ttcaggttcc gaccggctcg acggccgcca accgctacaa cgtcaatctg
     4981 ccggtctcca gccccgccgg cagtttcgcc ctcggcatcc tcggctcgaa ctacatcgtg
     5041 gatctcgagc tgtccgcggc ccaggccgag accaacgcgc agatcatctc ctcgccgcga
     5101 gtcatcacgg cgaatcagaa ggaggcgacc atcgagcagg gtacggagat cccgtatcag
     5161 cagtcggcct cgagcggtgc gaccaccatc tccttcaaga aggcagtgct ctcactgaag
     5221 gtgacgccgc agatcactcc tgacaaccgc atcattctcg acatcgatgt caagaacgac
     5281 agcatcggca ccatcaccgt cgcctcgggc ggcgtcaacg ttccctccat cgacacgaag
     5341 gagatcgtga cgcaggtgct gctgaatgac ggccagaccg tggtgctggg tggcgttctg
     5401 cagaccaccc atcgcgaaga ctcgacgaag gtgcctttcc tcggggacat ccccgtcctg
     5461 ggcaacctgt tcaagaccac atcgaaaacc aacaataagg atgagctgct gatcttcgtg
     5521 acaccgaaga tcgttcgcga aggggtcaac gtctactgat gcgggcggga accgcatcgg
     5581 caggaagttt tcaggtgagg acaatgcaca aatccttttc gatcccggca gcagcaatcc
     5641 tggcgctcgc gctttccgcc tgcggcggag gcgagaacct cagtggcggc tcgtccagcg
     5701 gcaccagcgg caccggtacc ggtaccggta cgggcacggg caccggcacg accacgactt
     5761 actcgatggg caacggcagc ggctcgagct ttcagacagg ggtcatctct ctgtccagca
     5821 cctctttgtc ggccggcggc acagccagcc tgaccgtgac catcgtcgat cagaccggca
     5881 cgctctatac cggtgctgcg gtcacggtga cattcaactc gccgtgcgtc gcgcagggac
     5941 ttgccacgat cacggcctcg ggcgccagca cggccgggag cagtccgggc accgttcaga
     6001 catcaactgg cagcgccagt gccacctaca ccgcgaaggg ctgtagtggc tccgatgtca
     6061 tcacggcaag cgcgcttgtc ggggggcaga acctgagcgc aacgggtacc gtttcggtcg
     6121 cggcggcctc gatcggctcg attcaattcg tgtccgcgac gccctcaacg atcgggttga
     6181 agggcacggg cctcaacgag acctcgaccg tgatcttcaa agtcacggac tcgactggcg
     6241 gcgaccggcc cggcgtgacg gtgacgttcg cgctggactc aactgtcggc ggcctcacga
     6301 tatcgcccac ctcggcgacc tctgcggcag atggcaccgt gcagacggtg gtgagctcgg
     6361 gtacggccca cagcacggtc cgcgtgaaag cctccattgc aagtccagcc ctttcgaccg
     6421 agtcgagcgc gttgacggtc agcacgggct tgccgtcctc caacgcgttc tccatctcgg
     6481 taggcccggc cacgtatggc accggtgcca gcacgctcgc gtgctccaac gttgaagcct
     6541 atgacattga tggtgtgacc gttccggtca ctgtccggtt ggccgaccgc tacaacaatc
     6601 cggcgccgga tggtacggca gtcgcgttca ccacggatgg cggccacatc gtcgcaagct
     6661 gcacgacgcc ctcttcgccc accagtccgg gagacgggac ctgcacggcg ctgtggacca
     6721 gtgccgatcc gcgcccgacg acctccagcg tcccgccggc cgccaacaat ggccgcgcta
     6781 tggtcatggc caccgccatc ggcgaggaat tcttcaacga tgccaacggc aacggctact
     6841 acgatccggg cgagacgttc gccaacctgg gagagccgtt ccgggacgac aacgaaaacg
     6901 gtacctatga tcacggcgag tacttcctgg atttcaacaa gaacggagtc cgggatcccg
     6961 gtgacgggac cttcaagggc attacgtgca cggcctcgag ctgcaccacg aataccctgg
     7021 cgatcagcgc ctctcacctc atcatcatgt ccacgagcgc ggctgcggtc acattcgtgg
     7081 gcgcgaccgg attcaccggg aatggctcag gcctgagcat cacacactcc acctcgggca
     7141 ccgtgacgat cggtgtggcc gactcgaacg gcaatgccat ggcggcaggc acgaaagtgg
     7201 cggtgtcggc cgataccggt gcagggaccg tcacgcagac gcccagcccc ttcattgtcg
     7261 gctgcgatgc gggcaggggt ggcgagcagg tgtcgttctc tgtggcagcc gccagctccg
     7321 ccggcggagg cacgatcacg attcaggtga cggcgcccaa tggaacgaac tccgtcttca
     7381 ccattcccgt cacggtcaac tgagtctcag gcgttcgatc gagtgcaagc ggccgccttc
     7441 gggcggccgc ttcatttcgg tatcctgcac agcgatgctg ggcaagccga acctgtttct
     7501 gatcggtccg atgggctccg gaaagagcgc cgtgggcaaa catctgtccc gcgttctgaa
     7561 cgtgccgttt cacgacagcg atgcggagat cgagcgccgc accggagtcg atatcccttt
     7621 catcttcgag aaggaaggcg aggcgggctt ccgggcacgc gagcgtgatg tcatcgaagc
     7681 cctgacggcg ctggagccga tcgtgctcgc caccgggggc ggggcggtgc tgctgcccga
     7741 gaatcgcaag gtgctcgccg agcgcggccg ggtcgtgtat ctggagactt cggttgcgca
     7801 acaggctgac cgtgtgcggc aaggccgggc gcgaccattg ctcaccaacg tggatcggca
     7861 ggcgaagctc gaagagctca tggacaagcg cggcacgctg tacgcgcaga tcgccgacct
     7921 cacggtcgcg accgacagcc gcaaggttca ctatgtggcg gagctgatcc tgcgcgagct
     7981 cgactcgaga gcgagaggct gatcggcgtc gcgctgcctg gggccgttgg ccggtggtca
     8041 tcgcggcagg gcgggctcgg ggcacgtata ctgggaggcg cgttgcgccc cgttgggttg
     8101 ggcgtttttg ttgcatgtgg atacgctaaa agtcgagctg ggcgagcgct cctatccgat
     8161 tttgatcggc gcggggctgc tgcaggaacc ggatcggatc cgggagcaca tcgatgcgcg
     8221 cgatgtgctg atcgtcagca actccgccgt cggacctttg tacgtgccgc ccgtcacggc
     8281 aggcttgagc gcccaacgag tcctcgaggc catcctgccc gatggggaag cccacaagac
     8341 actcaccacg gtcacccgca tcctggatgt cctggtggcc
//
LOCUS       DASRHN010000051         7459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2162592, whole genome shotgun sequence.
ACCESSION   DASRHN010000051 DASRHN010000000
VERSION     DASRHN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162592"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..376)
                     /locus_tag="VFB37_01925"
     CDS             complement(44..376)
                     /locus_tag="VFB37_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21232.1"
                     /translation="MAQYLIVAITFVFILGMIWLRSRLHYVERGGGVLRLQPAGRIYF
                     ASALGVLIIGWFVAPAVGRAIWPGAAITPTLMRVVWCLGTYYVYILVHRLLKSRGTMV
                     FRYEEPVP"
     gene            439..1047
                     /locus_tag="VFB37_01930"
     CDS             439..1047
                     /locus_tag="VFB37_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21233.1"
                     /translation="MTPPRSDNTVALDSHALGTLNYIRASIEAASAFAVPGIAGIAMG
                     AVGVGATVVSSIPVLAPYWLQIWLCAAIAASALGVLLVARQPARGAILYRGPARKFVL
                     SLCPALLAGAVLTAVLWQVDESKLIPGMWLLLYGCAVLSASLMTSAAIMRLIATMGGL
                     FVAYGLVAFELPPSWHNFILGLGFGALHLVFGILIGRSDRVE"
     gene            1088..1438
                     /locus_tag="VFB37_01935"
     CDS             1088..1438
                     /locus_tag="VFB37_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZO21234.1"
                     /translation="MSAPASRPVPVSTSSRSSEDARFDRLVYERVRLGIMSALAVNER
                     LTFNELKALFEVSDGNLSAHARKLEEADYIVCSKSFEGRRPKSEYRLTPVGRRALQRY
                     LEHIEAVIKATRRV"
     gene            1441..2172
                     /gene="uppS"
                     /locus_tag="VFB37_01940"
     CDS             1441..2172
                     /gene="uppS"
                     /locus_tag="VFB37_01940"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZO21235.1"
                     /translation="MENLHVAIIMDGNGRWAQQRGLPRTAGHVEGARTVRQIVEAAAQ
                     AGLRALSLYAFSADNWGRPGAEVMALMRLLRRYLLTETQRCVDQSVRINIIGRRDRLD
                     RSLVHAIERSERLTAGCQGLHLRIAVDYSAQYSILEAARRLARPENLHGRAMAEADCV
                     DTRRFQELLDQVAHSSASAGAVDLLIRTGAEQRLSDFLLWECAYAELYFTDCMWPDFG
                     ATGLRAALADYAGRQRRFGKVAEAR"
     gene            2274..4364
                     /locus_tag="VFB37_01945"
     CDS             2274..4364
                     /locus_tag="VFB37_01945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO21236.1"
                     /translation="MRLCPLLLVTLAATVGTVSAAPLEAPSHVFEGRDLFSLQCVTDP
                     QVRPDGHAVAYVRVSYEIMTDRPRRSIWLVDVDSGTDVPIAAGASSSYAPRWSPDGKR
                     LAYISSDGGHSQLYVRWMQSGQAARITDLTQAPNDLEWSPDGRSIAFIMLTPDEKSTL
                     GTAPAKPEGANWAEPLTVITELKYRADGQGYLKPGFTHAFVVSAEGGFPRQLTFGAFD
                     EEGPLSWTPDGQFIVLSGNRHEGWQHDPIKSELYQVSISDGAITELTHREGPDRNPRI
                     SPDGHRIAWLGFDDRLLGYQNVRVYLGDRQAQNSHSLTDKLDRSVDDVQWAGDGRSLY
                     IRYTDKAITKVARLSLDGRVEPVAEGLSGSGLDRPYTSGAFTVSSNGVVAFTAGTPEH
                     PSDLAVAHGTHVTRLTHLNDELFAVRKLGQVKHLEVTSSVDKRPIDAWLVTPPNFDAS
                     HKYPLILEIHGGPYASYGPVFSTDNQLYAAAGYLVLYANPRGSTSYGEDFANQIQHDY
                     PSHDYDDLMSAVDAAIATGSVDPEELFVTGGSGGGVLTAWIVGRTHRFRAAATDKPVI
                     NWGSFVLTTDISTYVRKYWFAKPPWEDPDTYWKHSPLSLVGNVTTPTMVVVGGEDYRT
                     PVSESEQYYEALQLRGVPTALVKVPGASHEGLASRPSQSAAKASATLAWFDRYRQGAA
                     HTGHPAPANANSGH"
     gene            4382..4858
                     /locus_tag="VFB37_01950"
     CDS             4382..4858
                     /locus_tag="VFB37_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZO21237.1"
                     /translation="MPMSLRVNDRPHELEIDPDMPLLWALRDHLRLMGTKFGCGMALC
                     GACTVHIDGKAVRSCVTPVSSVAGKSITTIEGLSSQAGKAVQAAWIHLDVPQCGYCQS
                     GQIMSAAALLADNSRPSDSDIDAAMAGNICRCATYVRIRAAIHEAAAALPLGSAGS"
     gene            complement(4904..5953)
                     /locus_tag="VFB37_01955"
     CDS             complement(4904..5953)
                     /locus_tag="VFB37_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO21238.1"
                     /translation="MDSPAPPARRVPKIRWGLRETLRSLGGGPGYDGPPSDHFDGHVF
                     FNPGSRGNRSFGDFLKWQRTREPKVWPRWVLNRARPELPPAVVPGQIALTFINHITFL
                     VQFAGLNILTDPVYSWRVSPFRSLGPQRVRDPGLAFEDLPPIHLVLVSHNHYDHLDLP
                     TLMKLEAVHAPHFITGLGNRPFLQQFGLRSVDELDWWESRSVAAANITMTPARHWSSR
                     RPRNRNRTLWGGFCVHSGGCQLYFAGDTGYGPHFREVRERFGQVNLALLPIGAYEPRW
                     FMSNQHMDPDEAVRAHLDLGAKVSVGSHFGCFQLTDEGIDDPIVELAAARQRHAVSEE
                     DFPVLNTGETRLFRR"
     gene            5977..6585
                     /locus_tag="VFB37_01960"
     CDS             5977..6585
                     /locus_tag="VFB37_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZO21239.1"
                     /translation="MRIERVETNTQRAVCVYCASSRASHPQYREAAFRLGEVLAGRGL
                     EIVYGGGARGSMGALADGALSRGGRVIGVLPRFMADLEWGHRGLSELELVEDMRARKH
                     LMLTRSHAAVALPGGSGTLEELLEAITLKRLGLYLNPIVLVNTRDFFDPLVHLLSRAI
                     EERFMDERHAQMWEVVAQPEEVPEALARAAAWDADARRFAAL"
     gene            complement(6707..>7459)
                     /locus_tag="VFB37_01965"
     CDS             complement(6707..>7459)
                     /locus_tag="VFB37_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO21240.1"
                     /translation="AERPLGFGAHGTDIESFRLRGVKIGAMPVAKSAHPQTARLYADA
                     RSALAQDGAVIVDLTAPKAFSEGEDAELQALLYEFKDSINAYLATLDPSQVDCRTLAD
                     LIAFNRDHSDAELIVFGQELFEMAEGRGPLTDGDYKKARATLDRTADKEGLATLFGQQ
                     GVEVLMSPSNGPAERIDEVWGDRHDGGFSEIASAAAIAGYPSLTVPMGTICGLPIGLT
                     LVGNRNQDGLILQVARAFERATNARLPPRLVI"
BASE COUNT         1317 a         2347 c         2485 g         1310 t
ORIGIN      
        1 tatcggaggt ggctggagtg atgagacgga gcggcgctga ggatcacggc accggctcct
       61 cgtagcgaaa caccatcgtg ccgcgactct tcaggagccg atgaacgagg atgtagacgt
      121 agtaggtgcc cagacaccag accacgcgca tgagagttgg tgtgatcgca gcacctggcc
      181 agatggcacg gcccactgcc ggtgcgacga accacccgat gatcagaacc ccgagagccg
      241 aggcgaagta gatccgcccg gcaggctgta atcgcagaac gccgccgccg cgttcaacat
      301 aatgaaggcg gctgcgcagc cagatcattc ccaggatgaa cacaaaggta atggcaacaa
      361 tcagatactg ggccatgtgt cttcactctg aaacgcagag tacttgacgg tttaacagtc
      421 cgctcctaga gtcatcccgt gaccccgcca agatcggaca ataccgttgc gctggacagc
      481 catgcgctcg ggacgctgaa ctacattcgg gcctccatcg aggccgccag cgcgtttgcc
      541 gttcccggaa tcgcgggcat cgcgatgggc gccgtgggcg tgggcgctac cgtggtcagt
      601 tcgattccgg tgctcgcgcc gtattggctc caaatctggc tctgcgccgc aatcgcggct
      661 tccgccctcg gcgtgctgct cgtcgcgcgc cagcccgccc gaggcgccat cctgtatcga
      721 ggtccggcgc gcaagttcgt tctgtccctc tgccccgcgc tgctcgcggg cgccgtgctc
      781 acggccgtgc tgtggcaggt cgatgagtcg aagttgattc cggggatgtg gctgctgctt
      841 tacggttgcg cggttctgtc ggcgagcttg atgacgagtg cggcgatcat gcggctgatt
      901 gccacgatgg gcggactgtt cgtcgcctat gggctggttg cattcgagct gccgccgagc
      961 tggcacaact tcatcctcgg gttgggcttc ggggccctgc acctcgtgtt cggtatcctg
     1021 atcggaagat cggaccgtgt cgaataagcc tgcggccagc agccccgcca ctcgaagcga
     1081 agaagggatg agcgcgcccg cgagccgacc tgtgcccgtg tccacctcct cgcggagcag
     1141 cgaggacgcg agattcgatc gcctggtcta cgagcgcgtg cgcctgggca tcatgagtgc
     1201 cctggcggtg aacgagcggc tcaccttcaa cgagctgaaa gcgctgttcg aggtgagtga
     1261 cggcaacttg agcgcgcatg cacgcaagct cgaggaggcc gactacatcg tctgctccaa
     1321 atccttcgaa gggcgccgac ccaaatccga ataccgcctg acgccggtgg gccgccgcgc
     1381 cctgcagcgc tatctggagc atatcgaagc ggtgatcaag gcaacgcgcc gggtctgacc
     1441 gtggaaaacc tgcacgtcgc gatcatcatg gacggcaacg gacgctgggc ccagcagcgc
     1501 gggttgccgc gaacggccgg tcacgtggaa ggcgcgcgga cggtccgcca gatcgtcgag
     1561 gccgcggcac aggcgggcct ccgcgccctg agtctgtatg ccttttcggc agataactgg
     1621 ggtcgccccg gcgcggaagt catggcgctg atgcgcctgc tgcgccgcta tctgctcacc
     1681 gagacgcagc gctgcgtgga ccagtccgtg cgcatcaaca tcatcgggcg gcgggaccgc
     1741 ctcgatcgga gcctcgtgca tgcgatcgag cgcagcgagc gcctgacggc cggctgccag
     1801 ggattgcacc tgcgcatcgc cgtggactat tcggcccagt acagcatcct ggaagcggcg
     1861 cgccggttgg cacgaccgga aaacctgcac gggcgcgcga tggcagaggc cgattgcgtg
     1921 gatacgcgtc gctttcagga gttgctcgat caggttgctc actcctccgc atccgccggg
     1981 gcggtcgatc tgctcatccg cacgggtgcg gagcagcggc tcagtgactt cctcctctgg
     2041 gaatgcgctt acgccgagct gtacttcacc gattgcatgt ggccggactt cggcgcgacg
     2101 ggcctgcgcg cggcactcgc cgactacgct ggacggcaga gacgtttcgg aaaggtcgct
     2161 gaagcgcgct gagggcaagc gcaccgcctg gaggccctcg gcactgggcg gcgtcgcctc
     2221 tataatcgcc ccccaccgcc ataccggcat gccgcacagc ttccggagcg ctcatgcgtc
     2281 tttgtccgct gcttctggtc actttggccg ctaccgtcgg cactgtcagc gctgcacccc
     2341 tcgaagcgcc ctcgcacgtg ttcgaggggc gcgatctgtt cagcctgcaa tgtgtcaccg
     2401 atccgcaagt gcggcccgat gggcacgcgg tcgcctacgt gcgcgtctcc tacgagatca
     2461 tgaccgatcg cccgcgacgc tctatctggc tggtcgatgt tgattccggc accgacgtgc
     2521 cgatcgctgc gggagccagc tcgagctatg cccctcgctg gtcacccgat ggcaagcgcc
     2581 tcgcctacat ctccagcgat ggtggacact cacaactgta tgtgcgctgg atgcagagcg
     2641 gccaagccgc acggatcacg gatctcacgc aagcgccgaa cgatctcgaa tggtctcccg
     2701 atgggcgctc gatagccttc atcatgttga cccccgatga gaagagcacg ctgggcaccg
     2761 cgccggcgaa gccggaaggc gcgaactggg ccgagccgct gacggtgatc accgagctca
     2821 agtatcgcgc ggacggccag ggttacctca agcccggctt cacgcacgcc ttcgtcgttt
     2881 ccgccgaagg cggctttcca agacagctga ccttcggtgc gttcgatgag gagggaccgc
     2941 tgtcctggac acctgacggc cagttcatcg tgttgagcgg caaccggcac gagggctggc
     3001 agcacgaccc catcaagtcc gagctctacc aggtctcgat ctccgatgga gcgattaccg
     3061 agctcacgca tcgtgagggt cccgaccgca atccacgcat ctccccggac ggccaccgga
     3121 tcgcctggct gggcttcgat gaccggctgc tcggatatca gaacgtgcga gtctatctcg
     3181 gtgatcgcca agcacagaac tcgcattccc tcacggacaa gctggaccga tcggtggacg
     3241 atgtgcagtg ggcgggcgat ggccgcagct tgtacatccg gtacaccgac aaggcgataa
     3301 cgaaggtggc gcgcttgtcg ctggatggtc gcgtggaacc ggtagccgaa gggctgagcg
     3361 gctcgggcct cgatcgccct tacacttctg gagcattcac ggtgtcctcc aacggcgtgg
     3421 tggcctttac cgccggtaca cccgagcatc cttctgatct ggctgtcgcg cacggaacgc
     3481 atgtcacccg cttgacgcac ttgaacgatg aactgttcgc ggtacgcaag ctgggacagg
     3541 tgaagcatct ggaagtgact tcatcggtgg acaagaggcc gatcgatgca tggctcgtga
     3601 caccgcccaa cttcgatgcg agccacaagt atccgttgat cctcgagatc cacggtggcc
     3661 cgtacgcaag ctacggcccg gtgttctcca ctgacaatca gctctacgcg gcggcgggct
     3721 acctcgtgct gtatgcgaac ccgcgcggct cgacctccta cggagaggac tttgccaacc
     3781 agattcagca cgactatccg agtcatgact acgacgatct catgagcgcg gtggatgccg
     3841 cgatcgccac gggatccgtc gacccggagg agctcttcgt gacaggaggg tcaggcggag
     3901 gcgtgttgac ggcgtggatc gtgggccgga cgcatcgatt ccgcgcggcg gcgacggaca
     3961 agcccgtgat caactggggc agctttgtcc tgaccacgga catctcgacc tacgtgcgca
     4021 agtactggtt cgccaagccg ccgtgggaag atccggacac gtattggaag cattcgccgc
     4081 tctcgctggt gggcaacgtg accacgccca cgatggtggt ggtcgggggt gaggattacc
     4141 ggacgccggt gagtgagtca gagcagtact acgaggccct gcagctgcgc ggggtcccga
     4201 ccgcgctggt gaaagtgccc ggcgcctcgc acgagggact ggcttcgcgg ccctcgcaat
     4261 cggccgccaa ggcgagcgcc acgctcgcct ggttcgatcg ctatcgccag ggcgccgcgc
     4321 acacgggcca tccagcgccg gcgaacgcga acagcggcca ctgacaccgg ggagagacgt
     4381 catgccgatg agcctgagag tcaatgatcg gccgcacgag ctcgagatcg atcctgacat
     4441 gccattgctg tgggcgttgc gcgatcactt gcgcctcatg ggcacgaagt ttggctgcgg
     4501 catggcgctg tgcggagcct gcacggtgca catcgatggc aaggcggtcc gctcgtgcgt
     4561 gacgccggtc tcctctgttg caggcaagag catcacgacg atcgaagggc tgtcgagcca
     4621 ggcgggcaag gccgtgcagg cggcgtggat ccatctcgat gtaccgcaat gcgggtactg
     4681 tcaaagcgga cagatcatgt ccgccgccgc actgctggca gataattcgc ggccgagcga
     4741 ttcggatatc gacgccgcca tggcggggaa catctgccgg tgcgccacct atgtgcgcat
     4801 ccgggccgcc attcacgaag ccgcggccgc gcttccgctg ggctctgctg gtagctgagg
     4861 gctcacaggg cgcaattcgc gaattgcgcg ctctcgctgt gcctcagcga cgaaagagcc
     4921 gtgtctcgcc ggtattcaga accgggaagt cttcttccga tactgcgtgc cgttgccggg
     4981 cggctgcgag ctccacgatg gggtcatcga tcccttcatc ggtgagctga aaacatccga
     5041 agtgcgagcc gacggagacc ttggccccca gatcgaggtg cgcacgcacc gcctcatccg
     5101 gatccatgtg ctgattgctc atgaaccagc ggggctcgta ggctccgatc ggcagcagcg
     5161 cgagattcac ctggccgaag cgctcgcgga cctcgcggaa gtgagggccg taaccggtgt
     5221 cgccggcgaa atagagctga cagccccccg agtgcacgca gaaaccaccc catagcgttc
     5281 gattgcgatt gcgcggccga cggctcgacc agtgcctcgc cggcgtcatc gtgatgttcg
     5341 ccgctgccac gctccgcgac tcccaccagt cgagctcatc gaccgagcgc aggccgaact
     5401 gttggaggaa gggacgattg cccaggccgg tgatgaaatg cggtgcgtgg acggcctcga
     5461 gcttcatgag cgtgggcagg tcgaggtgat cgtagtgatt gtgggaaacg agcacgagat
     5521 gaatcggtgg cagatcctcg aatgcgaggc cggggtctcg cacgcgctgc ggaccgaggc
     5581 tgcgaaaagg actcacgcgc caggagtaca ccgggtcggt gaggatgttg agcccggcga
     5641 actgcacgag aaaagtgatg tggttgatga acgtcagggc gatctggccc ggcacgaccg
     5701 cgggcggcag ctccgggcgg gcgcgattca gcacccagcg cggccagact ttgggctctc
     5761 gcgtgcgctg ccatttcagg aaatccccga acgagcggtt gccgcggctg cccggattga
     5821 agaacacgtg gccgtcgaaa tgatcggagg gcggaccgtc gtatccgggg ccaccgccga
     5881 gtgagcgaag cgtctctcgc aggccccagc ggatcttcgg gacacggcgg gccgggggtg
     5941 caggcgaatc catggtttcc ttgtggcgac agccgcatga gaatagagcg agtggagacg
     6001 aacactcaac gagcggtctg tgtgtactgc gcctcgagcc gggcttcgca tccgcagtat
     6061 cgcgaggccg ccttcaggct cggcgaggtg ctggccgggc gcgggctcga gatcgtatac
     6121 ggtggcggcg cgcgcggatc gatgggggcg ctcgcggatg gcgcgctgag cagaggcggc
     6181 cgtgtcatcg gggttctgcc gcgattcatg gcggacctgg agtggggcca ccggggtctc
     6241 tcggagctgg agctcgtcga ggacatgcgg gcgcgcaagc atctgatgct cacccgcagc
     6301 cacgccgcgg tcgccctgcc tggaggctca ggcacgctgg aggagctgct cgaggcgatc
     6361 actctgaaac gtctgggttt gtacttgaat ccgatcgtgc tggtgaacac gcgagacttc
     6421 ttcgatccgt tggtgcacct gctgtcgcgc gctatcgaag agcgtttcat ggacgagcgc
     6481 cacgcacaga tgtgggaagt cgttgcacag cccgaagagg tgccagaggc gctggcgcga
     6541 gcggcggcgt gggatgccga cgctcgccga ttcgcggcgc tctaggttaa ctggagaaag
     6601 cggctgcggc gccttctcca acatcggcta cgtttccggc ggcaaaacgc gtggcttttg
     6661 cggcctgtac agccccgcga tagcagcagt cctggcggct gcaggctcaa atcaccaggc
     6721 gaggcggcag gcgggcattc gtcgccctct cgaaggctct cgccacctgc agaatcaggc
     6781 cgtcctgatt gcgattgccg accagcgtga ggccgatggg cagtccgcag atggtgccca
     6841 ttggcaccgt caggctcgga tagcccgcga tcgccgcggc ggatgcaatc tcggagaacc
     6901 cgccatcgtg ccgatcgccc cagacctcgt cgatccgctc cgcggggcca ttgctcggcg
     6961 acatcagcac ttcgacgcct tgctgcccaa acagtgtcgc gaggccctcc ttgtcggcgg
     7021 tgcggtccag cgttgcccgg gctttcttgt agtcgccatc ggtgagcggg ccgcggcctt
     7081 cggccatctc gaacagctcc tgcccgaaga cgatgagctc tgcatccgag tgatcccgat
     7141 tgaaggcgat gagatccgcc agcgtgcggc aatcgacctg tgaggggtcg agggtggcca
     7201 gataggcgtt gatggaatcc ttgaactcat acagcagggc ttgcagctcg gcatcctccc
     7261 cttcgctgaa ggctttgggt gccgtcaggt ccacgatcac tgcaccgtcc tgcgccagag
     7321 cagagcgtgc gtctgcgtag aggcgtgcgg tttgcgggtg agcggatttg gcgaccggca
     7381 tcgcgccgat cttgacgccg cgcaatctga acgactcgat gtccgtgccg tgggcaccga
     7441 agcccagcgg acgttcggc
//
LOCUS       DASRHN010000052         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_513715, whole genome shotgun sequence.
ACCESSION   DASRHN010000052 DASRHN010000000
VERSION     DASRHN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513715"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFB37_01970"
     CDS             complement(<1..466)
                     /locus_tag="VFB37_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZO21241.1"
                     /translation="MLQEVVVTAQFRRQDLQQTPIAITAVNAAMLEQRNQTDISQVAA
                     QAPNVTLQPNGAAFGSSMVAFIRGIGQTDFDLALEPGVGIYVDDVYYATLTGSLLDLL
                     DLDRVEILRGPQGTLAGKNSIGGAIKLFTKKPTGEGGYLEGSYGSLHRVDARG"
     gene            complement(897..3398)
                     /gene="lon"
                     /locus_tag="VFB37_01975"
     CDS             complement(897..3398)
                     /gene="lon"
                     /locus_tag="VFB37_01975"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZO21242.1"
                     /translation="MSHFDIEGLSVSDPTTPVVPPSSSTPAPTLENVPVLPLRDVVVY
                     PHMVTPLFVGREKSIQALDVAMRTDKRIMLIAQKQADIDDPKGDDLYRIGTVATILQL
                     LKLPDGTVKVLVEGVDRARIERLHTAADYYSSDLTLLNAQQVYDEREMDVLARSVISQ
                     FEQYVKLNKKVPPEVLTALAGLDEVSRLADTVASHMSLKLAEKQKVLEILDVRKRLEH
                     ILVAIEGEMDVLQIEKRIRGRVKAQMEKSQREYYLNEQMKAIQKELGEMDEGGNEISE
                     LEQKIAKAGMPKEAREKAGGELNKLKMMSPMSAEATVVRNYIDWLVKVPWKKRTKIIK
                     DIARAEKVLDEDHFGLEKVKERIVEYLAVQQRVDKLRGPILCLVGPPGVGKTSLGQSI
                     ARATNRKFVRMSLGGVRDEAEIRGHRRTYIGSMPGKIVQNMSKAGVHNPLFLLDEVDK
                     MSMDFRGDPSSALLEVLDPEQNTTFNDHYLEVDLDLSEVMFVCTANSLNIPPPLLDRM
                     EVIRLPGYTEDEKMNIARRYLLPKQMKHNGLKEEELKVSDPTLLDIVRYYTREAGVRN
                     LEREIAKIGRKAVKELLLAKKDGKSVQVTPKNLEKYLGVRRFRYGKAEDENRIGQVTG
                     LAWTEVGGELLTIEAAVVPGKGKLQHTGQLGEVMQESIQAAMTVVRSRAQVLGLESEF
                     YQKHDVHLHIPEGATPKDGPSAGIGMCTALVSALTRIAVRSDVAMTGEITLRGEVLPI
                     GGLKEKLLAAHRGGIKTVLIPDENQKDLAEIPQNIKGNLEIKPVKWIDEVLAVALTQH
                     PTPQQADPPPGVAAAEDKRTRRNGRRAVKTVVPAH"
     gene            complement(3572..>4212)
                     /locus_tag="VFB37_01980"
     CDS             complement(3572..>4212)
                     /locus_tag="VFB37_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO21243.1"
                     /translation="EGTIASVPPQGGRKHPQQEFLQVDTQNILFICGGAFAGLEKIIL
                     NRTQKSGIGFTSEVRDVNVRPHGSDLFHDVEPEDLIKYGLIPEFVGRLPVVATLDELD
                     EAALISILTEPKNALTKQYRRLFEMEGADLEFRDEALRAVAHRAMQRRTGARGLRTIL
                     ETVLLDTMYELPSMRNVNKVVIDETVIEGQTKPYVVYRSEDEPRRASGTNHH"
BASE COUNT          723 a         1435 c         1199 g          855 t
ORIGIN      
        1 ctccgcgtgc atccacgcga tgcaggcttc cgtagctgcc ttcgagatag ccaccttcac
       61 ccgtaggctt tttggtgaac agcttgatcg cgccgccgat ggagttcttg ccggccaggg
      121 taccttgcgg tccacgcaga atctccactc gatccagatc gagcagatcc aacaaggatc
      181 cggttaatgt cgcgtagtag acgtcatcga cgtagatacc aacacctggt tcgagcgcca
      241 ggtcgaaatc ggtctgcccg ataccgcgga tgaacgccac catcgaggaa ccgaatgcgg
      301 cgccattggg ctgtagtgtt acgttgggag cttgcgcggc gacctgcgaa atgtccgtct
      361 gattgcgttg ttccagcatc gcggcgttca ccgcagtgat cgcgatcggc gtctgctgca
      421 ggtcctgcct gcggaactgc gcggtgacga ccacttcctg cagcacgtca ctactggcag
      481 tcgtggaggc ggcggtgttg tcagtagtct gggccaacgc cgaactggac aggagcccgg
      541 ccagacaagc gcaaagaacg aggcgagcgt aggaactcga agcccttgag ggcgcaactg
      601 gaaacagcat tagaccccct gtagcgattg agccgccgtc gtctgacgtc agccatcggc
      661 cagcgaggtg agcagcggct attccccccg cggatggatg ccgatcgcac gcgggttgtt
      721 gctgttgatc cgtggcggcg ccgcgcactc gtccgcgctg cggccgatca tccagcaagg
      781 tttcagccgt tacaagagat tacaagtgtg cgatgagggc cgggaacgcg cgcgtggacc
      841 gtttcccacg cgcacttccc cggtagacga atccttcaag tgaggcgcac tgaaactcag
      901 tgcgccggca ccaccgtctt gacggcgcgg cgaccgttgc ggcgcgtgcg cttgtcttcg
      961 gccgcggcca ccccaggggg aggatccgcc tgctgaggtg ttggatgttg agtgagcgcg
     1021 acggcgagca cctcatcgat ccacttgacc ggcttgatct ccagattgcc cttgatgttc
     1081 tgcgggatct ccgccaggtc cttctggttc tcatccggga tcagcaccgt cttgatcccg
     1141 ccgcgatgcg ccgccagcag cttctccttc aagcccccga tcggcagcac ctcgcctcgc
     1201 aaggtgatct ccccggtcat cgccacatcc gagcgcaccg caatgcgcgt cagtgccgac
     1261 accagcgccg tgcacatccc gatgcccgca ctcggaccgt ccttcggcgt ggccccttcc
     1321 gggatgtgca gatgtacgtc gtgcttctgg tagaactcgc tctccagccc cagcacctgc
     1381 gcgcggcttc gcaccaccgt catcgccgcc tggatcgact cctgcatcac ctcgcccagc
     1441 tgcccggtgt gctgcagctt gcctttgccg ggcaccaccg ccgcctcgat cgtcagcagc
     1501 tccccaccta cttccgtcca ggccagtccc gtcacctgcc cgatccggtt ctcatcctcg
     1561 gccttgccgt agcggaagcg ccgcacgccc aggtacttct ccagattctt cggggtcacc
     1621 tgcacgctct tgccgtcctt cttcgccagc agcagctcct tcaccgcctt gcggccgatc
     1681 ttggcgatct cccgctccag attgcgcacc cccgcctcgc gcgtgtagta gcgcacaatg
     1741 tccagcagcg tcgggtcgga gaccttcagc tcctcttcct tcagcccgtt gtgcttcatc
     1801 tgcttcggta acaggtaccg gcgggctatg ttcatcttct cgtcttccgt gtacccgggc
     1861 agccggatca cttccatccg gtccagcaac ggcgggggaa tgttcaggct gttcgccgtg
     1921 cacacgaaca tcacctccga gaggtccagg tccacttcca ggtagtgatc gttgaaggtc
     1981 gtgttctgct ccgggtccag cacctccagc agcgccgagg aggggtcgcc ccggaagtcc
     2041 atcgacatct tgtccacctc atccagcagg aacagcgggt tgtgcacccc cgccttggac
     2101 atgttctgca cgatcttccc gggcatcgag ccgatatagg tgcgccggtg accgcggatc
     2161 tccgcctcat cgcgcactcc gcccaaggac atgcgcacaa atttacggtt cgtcgcccgg
     2221 gcaatactct ggcccagcga ggtcttgccc actcccggcg ggcccaccag acacaggatc
     2281 gggccgcgca gcttgtccac ccgctgctgc accgccaggt actccacgat ccgctccttg
     2341 accttctcca gcccaaagtg gtcctcgtcc agcaccttct ccgcccgcgc aatgtccttg
     2401 atgatcttcg tgcgcttctt ccacggcacc ttcaccagcc agtcgatgta gttgcgcacc
     2461 accgtggctt ccgccgacat cgggctcatc atcttgagct tgttcagctc cccgcccgcc
     2521 ttctcccgcg cctccttcgg catccccgcc ttggcaatct tctgctccag ctccgagatc
     2581 tcgttccccc cctcgtccat ctccccgagc tctttctgaa tcgccttcat ctgctcgttc
     2641 aggtagtact cgcgctggct cttctccatc tgcgccttca cacgcccgcg aatccgcttc
     2701 tcgatctgca gcacgtccat ctcgccttca atggcgacca ggatgtgctc gaggcgcttg
     2761 cgaacgtcga gtatctcgag aaccttctgc ttctcggcca gcttcaagga catgtgtgag
     2821 gcgactgtgt cagccagacg gctcacctca tcgagccccg cgagtgcagt cagcacttcg
     2881 ggagggacct tcttgttgag cttcacgtac tgctcgaact gcgaaatcac cgaccgcgcg
     2941 agcacatcca tctcgcgctc gtcgtacacc tgctgagcgt tcaacagagt gaggtcggat
     3001 gagtagtaat cggcagccgt atgcaaacgc tcgatgcgag cgcggtcgac gccctccacc
     3061 aaaaccttga cggtaccgtc cggcagcttg agcaactgca gaatcgtggc gacggtgcca
     3121 atgcggtaga gatcgtcgcc cttggggtcg tcgatgtccg cctgcttctg cgcgatcagc
     3181 atgatgcgct tgtcggtacg catcgcgacg tccagcgcct gtatcgattt ctcacgcccc
     3241 acgaaaagcg gggtgaccat atgcgggtag acaacgacat cccgcagcgg aagcacagga
     3301 acgttctcca gagttggcgc cggagtgctg ctgctggggg gaacaacggg agtagtggga
     3361 tcagacacgc tcaagccctc gatgtcgaaa tgggacacgg cccttggtgc gagcaagggt
     3421 cgacagcgtc ggaagctagt ctaacgggaa atgaggtccg tccgccgtag ttcaacgagg
     3481 taggagtgcc gcggtccccc gcggctccgc cagcccgcgc agcggctctt gatctttcgc
     3541 gtgcgggaac cgcacgcgcg aaggcgtacc ttcagtgatg attcgttcct gaggcgcgcc
     3601 ggggttcgtc ttcggatcga taaaccacgt agggcttcgt ctggccctcg atgaccgtct
     3661 catcgatgac gaccttgttc acgttcctca tcgacggcag ctcgtacatc gtgtcgagca
     3721 ggaccgtctc gagaatcgtg cgcaagccgc gtgcgcccgt cctgcgctgc atggcgcgat
     3781 gggcgaccgc ccgcaaagcc tcgtcacgga actccagatc agcgccttcc atctcgaaga
     3841 gtcgccgata ctgcttcgtg agcgcgttct tcggctcggt cagaatcgag atcagcgccg
     3901 cctcgtccag ctcatccagc gtggccacga ccggcagacg ccccacgaac tccggaatga
     3961 ggccgtactt gatcaggtcc tcgggctcga cgtcatggaa caggtcgctg ccgtgcggcc
     4021 tgacgttgac gtcacggacc tctgaggtaa agccgatacc gctcttctgc gtgcggttca
     4081 gaatgatctt ttccagaccg gcgaacgcac cgccacagat gaacaagatg ttctgcgtgt
     4141 cgacctgcag aaactcctgc tgcgggtgtt tgcggccacc ctgaggcggc acggaggcaa
     4201 tcgtcccctc ga
//
LOCUS       DASRHN010000053        16527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1668725, whole genome shotgun sequence.
ACCESSION   DASRHN010000053 DASRHN010000000
VERSION     DASRHN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668725"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFB37_01985"
     CDS             <1..732
                     /locus_tag="VFB37_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO21244.1"
                     /translation="QFRKYADPAAEQLNDCGTLRWRTRNRFAAPLVVRMPGGFFKCGD
                     PWHSQTNEVAWVHGIGWRVAVPSNAEDAVGLLRSALRGNDPTIYFEHRAMLDGAWARR
                     PYPGDDFTVPFGVARLLRSGSELTVVSWGAMVERCEQAAADSAVDVELLDLRTLSPWD
                     REAVLSSVGKTRRCLIVHEDNITAGFGAEIAAVLAKDAFFQLDAPIERLTMPDVPSPH
                     SPVLLEAAVPSIERIRQSMQQIASV"
     gene            735..2096
                     /locus_tag="VFB37_01990"
     CDS             735..2096
                     /locus_tag="VFB37_01990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZO21245.1"
                     /translation="MSDVVEIRVPAEQTEGTRSQVLRWLKSVGEQVTADEPLIEIETD
                     KVTVEVAAPASGLLREILKQEQEEVGPGELLGRIEAEGTKSRQAPGAPTQSPGALSKP
                     VPTAWPAGARTEGPVATRSQAAEIEQPESGPRSDSGSARSLSPAVRRLLAEHGLEPSE
                     VHGTGQGTRITVEDILRHVKEPGAQGRPSALAARSGVRATTPAGDASPAAAEAVSERV
                     GAVRRVPHSAIRKRIADHMVHSLLHTAPHVTTVFEANLTAVLADRDRRRQEFAAAGVP
                     LTLTAYFVQAAAAAIRVVPEANSRWTDTALEIHESVHIGIATALENGLVVPVLHEVQS
                     RDLLQTARGLNDLTSRAREGRLTPADVRGGTFTISNHGVSGSLFAAPIVINQPQAAIL
                     GIGKLEKRVVVVDGVGAPVTQSMSIEPRCYVTLTIDHRVMDGLQANRFMQAFIRCLET
                     SGS"
     gene            2114..3430
                     /gene="paaK"
                     /locus_tag="VFB37_01995"
     CDS             2114..3430
                     /gene="paaK"
                     /locus_tag="VFB37_01995"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HZO21246.1"
                     /translation="MRTPDGTVPSELEAIERASEDELRTLQLARLKWSVRHAYANVAP
                     YRRKCELTGVHPEDLRTLDDLRHFPFTTKEDLRNSYPFGMFAVPRDKVTRIHASSGTT
                     GKPTVVGYTAHDIETWAQLVARSIRAAGGRRGDTVHVAYGYGLFTGGLGAHYGAERLG
                     CTVIPMSGGQTSRQVQLICDFEPDIIMVTPSYMLAIAEEFAHQNLDPAASSLRIGIFG
                     AEPWTDAMRLEIEKRMGLDAADIYGLSEVMGPGVASECIETKDGPVVWEDHFYPEIIE
                     PRTGHVLGDGREGELVFTSLSKEALPLIRYRTGDLTKLLRPTARSMRRIARISGRSDD
                     MLIIRGVNVFPSQIEELLLGLPGLTPHYVLELTREAHLDALTVHVECSAAIAQDERAR
                     ERLCQQLQYHIKERVGVTARVVVANPGAIERSAGKAKRVIDKRALR"
     gene            complement(3533..3667)
                     /locus_tag="VFB37_02000"
     CDS             complement(3533..3667)
                     /locus_tag="VFB37_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21247.1"
                     /translation="MLLEPQGIVPDRGLSHIRAEVEVRLPEAGVEILCEPRGRCGENQ
                     "
     gene            complement(3714..4712)
                     /gene="paaX"
                     /locus_tag="VFB37_02005"
     CDS             complement(3714..4712)
                     /gene="paaX"
                     /locus_tag="VFB37_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HZO21248.1"
                     /translation="MEPKPISPITPAAAALARATQGLLRRFRSHRPLRGGSLLVTIFG
                     DAIAPRGGIVTLGSLIRLAAPFGLTERLVRTSVGRLAQEGWLVARRDGRRSEYRLSGY
                     GEQSFAEATRRIYAASPSEWDGKWMLLLLPAKGPRREAGRRDELRQELRWLGFGQVSS
                     GVFLHPGCTPERARDWLRGLEEAKDAVLLQSFGDAGAADRRLVAAGWDLAELGRRYRA
                     FVAAFTPVRTAMHVPKSSKAVLMASDGMPGREVAFVIRTLLIHEYRKIHLQDPSLPPQ
                     LLPAHWIGTSAYELCRSLYGSVFAAAEDYLSNTASTLSEALPAAGREAYERFGGIG"
     gene            4801..5802
                     /gene="paaA"
                     /locus_tag="VFB37_02010"
     CDS             4801..5802
                     /gene="paaA"
                     /locus_tag="VFB37_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HZO21249.1"
                     /translation="MYTQGLDSLGKEADHHAGADTAALEARFQSRVDADEKIEPKDWM
                     PDAYRQTLIRQISQHAHSEIVGMLPEGNWITRAPTLRRKAVLIAKVQDEGGHGMYLYS
                     AAETLGIERAQMLEQLLAGTAKYSSIFNYPTLTWADVGAIGWLVDGAAIMNQIPICRC
                     SYGPYARAMVRICKEESFHQRQGFEIMLALARGTPQQHAMAQDALNRWWWPSVMMFGP
                     SDADSKHTAQSMRWKIKRFSNDELRQKFIDITAPQARFLGLTIPDPELRWDEQTQHFH
                     FGVIDWSEFQAVLKGHGPCNRERLEARRRAHEEGSWVREAAAAHAAKQQARSQQAAA"
     gene            5824..6108
                     /gene="paaB"
                     /locus_tag="VFB37_02015"
     CDS             5824..6108
                     /gene="paaB"
                     /locus_tag="VFB37_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HZO21250.1"
                     /translation="MSNDWPLYEVFIRSRSGLEHKHVGSLHAADSKMAVDHARDVYTR
                     RQEGVSIWVVRSADIIASDPLEADALFEPARDKVYRHPTFYAIPDEVKNL"
     gene            6105..6929
                     /gene="paaC"
                     /locus_tag="VFB37_02020"
     CDS             6105..6929
                     /gene="paaC"
                     /locus_tag="VFB37_02020"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HZO21251.1"
                     /translation="MSAVGEAAESPRGKPGSGESSPEGLRPALFRYLLRLGDTSLVLG
                     QRLGEWVGHSPALEEDLGLANISLDLIGQARLLLTYAGGIEGKGRDEDALAFLRDAPS
                     FANLTLVEQPNGDFGRTIVRQCLLDAWQLELYEALASSRDARLSSIASKAVKETRYHF
                     HYSSDWLVRLGDGTAESHGRVQRALEDLWRFTEELFAADEVEEQLGRAGVAPVLADLQ
                     PRWSSRMDAALHEATLVKPPPSPYPWHGKRGVHTEHLGHLLAEMQHLQRTYPGARW"
     gene            6972..7514
                     /gene="paaD"
                     /locus_tag="VFB37_02025"
     CDS             6972..7514
                     /gene="paaD"
                     /locus_tag="VFB37_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HZO21252.1"
                     /translation="MNWPGRAQAAEPARQPGTENIWALLERVPDPEIPVLSVVDLGIV
                     RHVEWEAAAGNPKILHVGITPTYSGCPATEMIHQSVRRALDAAGYTDALIEQVLAPPW
                     SSDWLTEAGRQKLAAFGIAPPESAVGQPRHLLRAPASVTCPHCGSRVTKRVSEFGSTP
                     CKAHYRCAACLEPFDYFKCL"
     gene            7645..8808
                     /locus_tag="VFB37_02030"
     CDS             7645..8808
                     /locus_tag="VFB37_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZO21253.1"
                     /translation="MLKFHPLRITGLHPEGQDAIALSLEVPQELRAEYTGRAGQHIVL
                     RKQFEAEEVRRTYSLVNTPGEWPLRIVARVHEKGLMSRFFAEQLRVGDSIDVLPPNGS
                     LTPHLGAYRAGAMYVAFAAGCGITPVLSIAKAILQSDGANQLILFYGNTSSERTMCLE
                     ELLALKDQYVQRLALHFVMSREPQEVELYNGRLDGERVRLFARLLFKPEEIAEYFICG
                     PGDMIDQLGATLRDLGVGAEHLHGEHFTLGAGVAPRAAAPAAAPAAPASRDSRTSTAV
                     GAAESIDGRGSQPASAADTAEVTVLMDGRRRTFTMKMNEDTVLDAAARAGLELPFSCR
                     AGVCSTCRTKVLSGKVEMAQNYALEDWELEQGYILACQSRVMTPVLELDYDEK"
     gene            8848..9639
                     /gene="paaG"
                     /locus_tag="VFB37_02035"
     CDS             8848..9639
                     /gene="paaG"
                     /locus_tag="VFB37_02035"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HZO21254.1"
                     /translation="MNYQTILFETTAGVARLTLNRPDRLNSFNSAMHQEVRDAIASLI
                     GDSGARVLVITGSGRGFCAGQDLGDRAVAPGGAAADLGESIEKNYKPLVLALHSLQIP
                     VIAAVNGVAAGAGANIALACDLVIAARSASFVQAFCKLGLVPDSGGTWFLPRLLGTAR
                     AMGLSLLGDKLPAQQAAEWGLIWRCVEDAELRAVVDELAAQLAAAPTRGLARTKQALY
                     ESWGRTLEQQLDVERDYQRELGRTTDYAEGVAAFTAKRAPKFTGR"
     gene            9649..10107
                     /gene="paaI"
                     /locus_tag="VFB37_02040"
     CDS             9649..10107
                     /gene="paaI"
                     /locus_tag="VFB37_02040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HZO21255.1"
                     /translation="MADTNDPAQQLAERAARAMFARDRAARMLGMRVSRVGPGHAEVL
                     MQVVPEMINGHDVCHGGLVFSLADTAFAYACNSYNESTVAAAGSIDFLAPARSGDALT
                     AVAQELWRTRRSGLYEITVTNQRGERIALFRGRSHRISGKVAPQESSETQ"
     gene            10104..12158
                     /gene="paaZ"
                     /locus_tag="VFB37_02045"
     CDS             10104..12158
                     /gene="paaZ"
                     /locus_tag="VFB37_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HZO21256.1"
                     /translation="MRDAPRQLQSYASGQWVAGSHDAVALRDATTGAVIASSSASGLD
                     YRGMLEHARQVGGPNLRRLTFHERAGLIKELAKRLGELKEEFYKLSFATGSTRSDSWI
                     DIDGGISTLFVYASKGTRELPNSCVYVDGGVEALSRGGTFVGQHICVPLEGAAVQINA
                     FNFPVWGMLEKLAPALLAGVPSIVKPATATSYLTEQVVRRIVETGLLPEGAIQLICGG
                     TGDLLEHLSCQDVVCFTGSAATAQKLRQHPAIMAHAVRFTSETDSLNSSILGPDAAAG
                     TPELELFVREVIREMTVKAGQKCTAIRKAIVPRERVADVVDAMKAALGQVIVGDPRLE
                     QVKMGPLASLGQRREVREQLARLQREAELVYGAQETEPVGADGERGAFLSPKLLYCRD
                     SASAREVHSVEAFGPVCTILGYDTLDTAIALARRGGGSLAASVFSADEQAAARLVLGL
                     APFHGRVLVVNRHCAKESTGHGSPLPHLVHGGPGRAGGGEELGGIRGVLHFMQRTAVQ
                     GTPDVLTAVTGRWAKGARQIDPGVHPFRKPFGALVIGDTFNSGEREVTIEDIERFAEL
                     SGDRFYAHMDEEAARRNPLFGGRVAHGYFLVSAAAGLFVDPPYGPVLANYGIDGLRFT
                     KPVKPGDRIKVRLTCKEKSLRLGMGYGEVRWDTQISNQNGEVVASYDVLTMVSEKSVP
                     DA"
     gene            12155..13036
                     /locus_tag="VFB37_02050"
     CDS             12155..13036
                     /locus_tag="VFB37_02050"
                     /inference="COORDINATES: protein motif:HMM:NF005595.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO21257.1"
                     /translation="MKRVTDKARPAKRPRSAATGSAPAEERVNTVVERYRSRFPALQF
                     SQPAAAVLEVVLANPARLNSADASMHRELASVWREIDADEAVRAVLVRGEGAHFSSGG
                     DFSLIESMIEDEATLVRVWKEASDLVYNLINCSKPVVSAIRGTAVGAGLAVALLADIS
                     IAGRDVKILDGHTRLGVAAGDHAVIIWPLLCGLAKARYYLLTNEPLSGEEAERIGLVA
                     RCVADEEVYPTALQVAARLAQGSSTAMRWTKHALNNWLRLAGPSFDTSLALEFLGFRL
                     HDARAGLEAVRNRPKRR"
     gene            complement(13011..14123)
                     /gene="modC"
                     /locus_tag="VFB37_02055"
     CDS             complement(13011..14123)
                     /gene="modC"
                     /locus_tag="VFB37_02055"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HZO21258.1"
                     /translation="MLKVAVLKRLGSFTLRAQFEAPTPGVVALFGRSGSGKTTTVNIL
                     AGLLAPDAGRVELNGSVLEDTATGMRIAAERRCIGYVFQEPRLFPHLSVLGNLQYGQK
                     RAAPAAQPIELERVVALLGLKPLLRRRPHQLSGGERQRASLGRALLSQPRLLLLDEPL
                     ASLDVARREEVLPYLELLRDSLSIPMVYVSHQLDEVLRLATYVVLLEGGRVAGQGTVN
                     EISLLPPLRSLVGPDAVGAVVDGQVTRFDGTQRMAELQVGRGKLHVSVREVTAGTRVR
                     VHLLARDIILGLQEPKGLSVRNALQGTIVELLDDDHETVLVKVDIGAATIVLARITRS
                     AAVELALRPGMSIWVLVKAVSTRGHAFRLSGASGDS"
     gene            complement(14141..14836)
                     /gene="modB"
                     /locus_tag="VFB37_02060"
     CDS             complement(14141..14836)
                     /gene="modB"
                     /locus_tag="VFB37_02060"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZO21259.1"
                     /translation="MHEILSPAEWEALRLSLEVALCSALFSLPWALLIAWVLSRGRFP
                     GRSVLDAFVHLPLVLPPVAVGYVLLLLFGNRGPLGGWLHAHFGVQLAFTTAGAALATA
                     VMSFPLMVRSIRISLESIDRGFEEAAQTLGAGPLDRFCTITLPLMLPGVIAGAVTAFA
                     AGLGEFGAVITFAANVPGETRTIPLALYTAIQSPTGDAQALRLAILSFALGLLGLMLA
                     EVLARGVRRKLGR"
     gene            14989..15414
                     /locus_tag="VFB37_02065"
     CDS             14989..15414
                     /locus_tag="VFB37_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZO21260.1"
                     /translation="MSIDRQFGWMWAQACELLDQADRMQRQFVRYVGQDAEAAVWEPP
                     VDIQESAEGVHFTFALPGVDPDRINVSLDPTALTVSAVRVPRLGGSGSVVRRLEIPHG
                     RFVRRIALAAARLRLGESRYSNGCLEVRLVWTDDNRGCK"
     gene            15416..>16527
                     /locus_tag="VFB37_02070"
     CDS             15416..>16527
                     /locus_tag="VFB37_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZO21261.1"
                     /translation="MADTENPASTAEPTSPPSDAIPVIAMRTVVVFPGVLAPVALGRE
                     ESIAAAQEAVRHGRKIGLLLQQDPAVEKPTAHDLHPIGVLASVVRYVTAPDGTHHLVA
                     QGESRFRAVEFVQERPYLAARTEVLAPANTTGPEIEARVEVLKQRAIEALSLLPQVPT
                     ELTRTIQSIDSASQLSDLIVSFMDVKVQDKQEMLETLDLRERLDKVLSLLSNRLQVLK
                     ITRDITEQTQSALGERQREAVLREQMRQLQRELGESEDSGSEVAELEKAVERAGMPAE
                     VAEHTRKELKRLQRMSEASPEYGMVRSYLDTLVALPWSTADAEDIDIERARRILDEDH
                     YGLDKVKRRILEYLAVRKLNPQGRSPILCFVGPPGVG"
BASE COUNT         2905 a         5317 c         5574 g         2731 t
ORIGIN      
        1 cagttccgca agtacgccga tccggcggcc gagcagctca atgattgtgg gaccttgcgc
       61 tggagaacgc ggaaccggtt tgcagccccg ctggtggtgc gaatgcccgg gggatttttc
      121 aaatgcggcg atccctggca cagccagacc aacgaggtgg cctgggtaca cggaatcggc
      181 tggcgcgtgg cagtaccctc caacgccgag gacgccgtcg gattgctgcg cagcgcgttg
      241 cgcgggaacg acccgacgat ctacttcgaa caccgcgcca tgctggacgg cgcctgggca
      301 cgccggccct atccgggcga tgacttcacg gtgccctttg gcgtggcccg cctgctgcgt
      361 tccggctcgg agttgaccgt ggtgagctgg ggtgcaatgg tcgagcgatg cgagcaggcg
      421 gcggccgact ccgcggtgga cgtcgaactg ctggacctgc gaacgctctc gccctgggat
      481 agggaggccg tgctgagctc cgtgggcaag acccgccgtt gcctgatcgt gcacgaggac
      541 aacatcacgg ccggcttcgg ggcggaaatc gctgcggtgc tggccaagga tgcattcttt
      601 cagctggatg ccccgatcga gcggctcacg atgcccgatg tgcccagtcc gcacagcccg
      661 gtgttgctgg aggcagcagt cccgagcatc gagcgcatca ggcaatccat gcaacagatt
      721 gccagcgtgt agccatgagc gatgtggtgg aaatccgggt gccggcggaa cagaccgagg
      781 gtacgcggtc gcaggttctg cgctggctca aatcggtcgg cgagcaggtc acggccgacg
      841 agccgctgat cgagatcgag acggacaagg tcactgtgga agtggccgcg cccgcatcgg
      901 gcctcctgcg cgagatcctc aagcaggagc aggaggaggt cgggcccgga gagttgctgg
      961 gacgaatcga agccgaagga accaagtcca ggcaggctcc gggtgcgccc acgcaaagtc
     1021 cgggtgcgct ctcaaagccg gtgccaacgg cgtggcccgc aggggcccgc accgagggtc
     1081 cggtggcaac tcggtctcag gcggcggaga tcgagcaacc tgaatccggt ccccgcagtg
     1141 acagcggtag cgcgagatcg ctcagccctg cggtccgcag attgcttgcc gagcacgggt
     1201 tggagccatc cgaagtgcac ggcacggggc agggcacgcg catcaccgtc gaagacatcc
     1261 tgcgccatgt gaaggagccg ggcgcgcagg gtcgcccctc cgcgctcgct gcacgatcgg
     1321 gtgtgcgtgc gacaacaccc gcgggcgatg cttcgcccgc tgcggccgaa gcggtcagcg
     1381 aacgcgtcgg cgcggtccgt cgcgttcctc attcggccat ccgtaagcgc atcgccgatc
     1441 acatggtgca cagcctgttg cacaccgcgc cgcatgtgac gacggtgttt gaagcgaacc
     1501 tcaccgcggt gctggccgat cgcgacaggc gccggcagga attcgccgcg gcgggtgtgc
     1561 cgctcacgct gacggcttat ttcgtgcagg ctgcagcggc agccatccgg gtggtgcccg
     1621 aagccaacag ccgctggacc gacaccgctc tggagatcca cgagtcggtg catatcggga
     1681 tcgccaccgc gctcgaaaac gggctcgtgg tgccggtact gcatgaggtg cagtcccggg
     1741 acctcctgca aaccgcgcgc gggctcaacg acctgacatc gcgggcgcgg gagggccggc
     1801 tcacaccggc cgacgtccgg ggtggcacgt ttacgatctc gaatcatggc gtcagtggca
     1861 gtcttttcgc tgcaccaatc gtgatcaacc agccgcaggc tgcgatcctg ggcatcggta
     1921 agctcgagaa gcgcgtcgtc gtggtggacg gcgtgggcgc ccccgtcacc cagagcatga
     1981 gcatcgagcc gcgctgttat gtcacgctga ccatcgatca ccgggtgatg gatggcctcc
     2041 aggcgaaccg cttcatgcag gccttcatac gatgtctgga gacttcaggc tcatagtgcg
     2101 atgatccgcc gctgtgcgga cccccgacgg caccgtccct tccgaacttg aggccataga
     2161 gcgtgcgagt gaggatgagc tgcgaacgct gcagctcgcg cgcctgaaat ggtccgtacg
     2221 ccacgcgtac gcgaatgtcg ccccctaccg ccgtaaatgc gagctgaccg gggttcatcc
     2281 ggaagacctg cgcacgctgg atgacctgcg ccacttccct ttcacgacca aggaagatct
     2341 gcggaacagc tatccgttcg gcatgtttgc ggtgccgcgg gacaaggtca cccgcattca
     2401 tgcttccagc ggaacgacgg ggaaaccaac cgtcgtcggc tacaccgcac atgacatcga
     2461 aacgtgggcg cagctcgtcg cgcgctcgat ccgagccgcc gggggccggc gcggcgacac
     2521 ggtgcacgtc gcttatggct atggcctgtt taccggcggc ctcggcgcgc attacggcgc
     2581 ggagcgcctg ggatgtaccg tgattcccat gtcagggggc cagacctcgc ggcaggtgca
     2641 gctcatctgc gacttcgagc ccgacatcat catggtcacc ccctcctaca tgctggccat
     2701 cgccgaggag ttcgcgcacc aaaatctgga tcctgcggcc tcatcgctgc gtatcgggat
     2761 cttcggcgct gagccctgga ccgacgccat gcgcctggag atcgagaaac ggatgggcct
     2821 cgatgccgcc gacatctatg gactgtcgga ggtgatggga ccgggtgtcg ccagcgaatg
     2881 cattgaaacc aaggacgggc cggtggtctg ggaggatcac ttctatccgg agatcatcga
     2941 gcctcgcacc ggtcacgtgc tcggtgatgg acgcgagggc gagctggtgt tcacttcgct
     3001 ctccaaggag gcgctgcctc tgatccgcta tcggacgggg gacctcacca agctgttgcg
     3061 accgactgcc cgctcgatgc gccgcatcgc acgcatcagc ggccgctcgg atgacatgct
     3121 gatcatccgc ggtgtcaacg tgtttccgag tcagatcgag gaactgttgc tcggcttgcc
     3181 aggactgacc ccccactatg ttctggagtt gacgcgtgaa gcgcacctcg atgcgctgac
     3241 ggtgcatgtg gagtgctccg cagcaattgc gcaagacgag agagctcgcg agcggctgtg
     3301 ccagcagctg caataccaca tcaaagagcg agtaggtgtc accgcacgcg tggtcgtcgc
     3361 caatcccggc gcgatagagc gttccgccgg caaggccaaa cgcgtcatcg ataagcgggc
     3421 gttgcggtag attccggccg cctggcggcg ctgcctggtc aacctggcgt ttgctcaggc
     3481 gttgctgaag gacccgccaa ggaggactcc tcatgcgcaa gtgctgcccg cgctactgat
     3541 tctcgccgca gcgcccccgg ggctcgcaca ggatttcgac tccagcttcc ggcagcctca
     3601 cttctacctc ggcgcggatg tgggagagtc cacgatccgg gacaattccc tggggttcga
     3661 gcagcatggg atcggcttcg acctgcgcgc ctcagcggcc acgtccggcc gctctagccg
     3721 attcccccaa accgctcgta ggcttcgcgg ccggctgccg ggagtgcctc gctgagcgtg
     3781 ctcgcagtgt ttgacaggta gtcctcggcc gccgcgaata cgctgccgta caagctccgg
     3841 cacaattcgt aggcgctggt gccgatccag tgtgcaggca gcagttgcgg cggtagtgag
     3901 gggtcctgca ggtgaatctt gcggtactcg tgaatgagca gcgtgcggat gacgaatgcg
     3961 acttcgcggc cgggcatgcc atcggatgcc atcaggacgg ccttggagga tttgggaacg
     4021 tgcatggccg ttcgcacggg cgtgaaggcc gccacgaacg cccggtagcg gcggccgagc
     4081 tcggcaagat cccaaccggc ggcgaccagc cgccggtcag cggcccccgc gtctccgaag
     4141 ctctgcagca gaacggcatc cttggcctcc tccagacccc gcagccaatc gcgggcgcgc
     4201 tcgggcgtgc accccggatg cagaaaaacg cctgagctca cctgcccaaa gcccagccag
     4261 cgcagctcct ggcgcagctc gtcgcgtcgc cccgcctcac ggcgcgggcc tttcgccggc
     4321 agcagcagca gcatccattt tccgtcccac tcgctcggac tggccgcgta gatgcgacgc
     4381 gtggcttccg caaaactctg ctctccatac ccgctcaggc gatactcgct gcggcgaccg
     4441 tcgcgtcggg caacgagcca accctcctgc gcaaggcggc caacggaggt gcggacgagc
     4501 cgctcagtca gtccgaaagg cgcggccagc cggatcagac ttccgagcgt cacgatgccg
     4561 ccgcgcggcg cgatcgcatc accaaagatg gtcaccagca gcgagccgcc tctgaggggg
     4621 cgatggctgc ggaagcgtcg caggagcccc tgcgtggccc gggcgagggc ggctgccgcg
     4681 ggggtgatcg gcgatatggg cttgggctcc atctgcagcc cgattgtgac acagaaatct
     4741 ttgcacgacg cctgtttctg tgtcacattg gcggcacaga tcctcgaccg cggagctgcc
     4801 atgtacacgc aaggactcga ttccctcgga aaggaggccg accaccacgc cggcgccgat
     4861 accgcagcgc tcgaggcgcg gttccagagc cgggtggacg ctgatgagaa gatcgagccc
     4921 aaggactgga tgcccgatgc ctatcggcag acgctcatcc gccagatttc ccagcacgcc
     4981 cactcggaga tcgtcgggat gcttcccgag ggcaactgga ttacgcgggc gcccacgctg
     5041 cgccgtaagg ctgtcctgat cgcgaaagtg caggacgagg gcggccacgg catgtacctc
     5101 tacagtgctg ccgagactct cgggatcgag cgtgcacaga tgctggagca gcttctcgcg
     5161 ggtaccgcga agtactccag catcttcaat tacccgactc tgacctgggc ggatgtcggg
     5221 gcgatcggct ggctggtcga tggtgcggca atcatgaatc agatcccgat ctgccgctgc
     5281 tcctatggcc cctacgcccg cgcgatggtc aggatctgca aggaagagag ctttcatcag
     5341 cggcaggggt tcgagatcat gctcgcgctc gcccgcggga cgccacaaca gcatgccatg
     5401 gcccaggatg cgctcaatcg ctggtggtgg ccctcggtga tgatgttcgg gcccagcgat
     5461 gcggactcga agcacacggc gcaatcgatg cgctggaaaa tcaagcgctt cagcaacgat
     5521 gaactgcgcc agaaattcat cgacatcacc gcccctcagg cccggttcct cggtctgacc
     5581 attcccgacc ccgagctgcg ctgggacgag cagacgcagc actttcattt cggcgtcata
     5641 gactggagcg agtttcaggc ggtgttgaag ggacacggtc cctgcaatcg cgagagactc
     5701 gaggcacgac ggcgtgccca tgaggaggga agctgggtgc gggaggctgc cgcagcccat
     5761 gctgccaaac aacaagcccg ttcgcagcag gctgccgcct gagtgagatc cgagggaaca
     5821 tccatgtcga atgactggcc gctgtacgaa gtcttcatcc gctcccgctc ggggctggag
     5881 cacaaacacg taggcagtct gcacgccgca gacagcaaga tggccgtcga ccacgcccgt
     5941 gatgtgtata cgcgccgcca ggaaggcgtg agcatctggg tcgtgcgctc ggcagatatc
     6001 attgcctcgg atcctctcga ggcggacgca ttgttcgagc cggcgcggga caaggtctac
     6061 cggcacccga ccttctatgc catcccggat gaggtcaaga acctgtgagc gcggtcgggg
     6121 aagccgccga aagcccgcga ggcaagccgg gtagcggtga gagcagcccg gaggggctgc
     6181 gaccggcgct cttccggtac ctgctgcgac tcggcgatac gagcctggtg ctcggccagc
     6241 gcctcggaga gtgggtgggg cactcgcccg cgctggagga agatcttggc cttgcgaaca
     6301 tctcgctcga tctcatcggg caggcgcgcc tgcttctgac ctatgcgggc gggatcgagg
     6361 gcaaggggcg cgacgaggat gcgctggcct tcctgcggga cgccccgtcg ttcgccaacc
     6421 tcactctggt cgagcaaccc aacggtgact tcggccggac catcgtgcgt caatgcctgc
     6481 tggatgcctg gcagctcgag ctgtacgagg cgctggcgag ctcgcgcgat gcgcggctct
     6541 cgagcatcgc ctccaaggca gtcaaggaaa cccggtacca ctttcattac agctcggact
     6601 ggctggtccg tctcggcgat ggcactgccg agagtcatgg ccgggtgcag cgcgccctcg
     6661 aggatctttg gcgattcacc gaggagctgt tcgcggccga tgaggtggag gagcagcttg
     6721 gtcgggcggg cgttgcgccg gtgttggctg atctgcagcc gcgctggtcg tcgcgcatgg
     6781 acgcggcgct gcacgaagcg acgctcgtga aaccaccacc gtcaccgtat ccctggcatg
     6841 gcaagcgcgg ggtccacacg gagcatctcg ggcacctgct ggccgagatg caacatctgc
     6901 agcgcactta ccccggggcc cgctggtaac cgcaagtgac gagtgctaca ccaacggttg
     6961 gcccaagggc cgtgaactgg ccagggcgag cgcaggctgc cgagccggct cggcagccgg
     7021 gcacggaaaa tatctgggct ctactcgagc gcgtgccgga tccggagatt ccggtcctga
     7081 gcgtggtgga tctgggcatc gtgcgtcacg tcgagtggga agcggctgcg gggaatccga
     7141 agattctgca cgtcggaatc actccgacct attcgggctg tccggcaacg gagatgatcc
     7201 atcagagcgt tcgtcgcgca ctggatgctg cgggctacac cgatgcgctc atcgagcagg
     7261 tgctggcgcc tccctggagc agcgactggc tgaccgaggc cggccggcag aagttggccg
     7321 ccttcggcat cgcaccgccc gagagcgcgg tgggtcaacc caggcatttg ctcagagcgc
     7381 ccgcctcggt aacctgtccg cactgcggca gccgcgtaac gaagcgcgtg agtgaattcg
     7441 gctcgactcc ctgcaaggct cactaccgct gcgcggcgtg cctggagcct ttcgactatt
     7501 tcaaatgtct atgacgatcc atgacagggg ggcggcacct gctcaggtgc tcagcgggag
     7561 cgcacattgc gctgcgctgt cctgctgcgc gcaagcccgg ggcgcacatc cgtgtgggcc
     7621 cgcgcggcgc catgcgcctc gcccatgctg aagtttcatc cactgcggat cacgggcctg
     7681 catcctgaag ggcaggatgc gatcgctctt tcgctcgaag tgccgcaaga gctgcgcgcg
     7741 gagtacacgg ggcgggccgg ccagcacatc gtgttgcgca agcagttcga ggcggaggag
     7801 gtgcgccgca cctattctct cgtcaacacg ccgggggaat ggccgctgcg catcgtggca
     7861 cgcgtgcatg agaagggcct catgtcgcgt ttctttgccg agcagctgcg agtcggagac
     7921 agcatcgatg tgctgcctcc caacggcagc ctcactccgc acctgggcgc gtaccgtgcg
     7981 ggcgccatgt atgtcgcatt cgcagcgggc tgtggcatca ctccagtgct gtccatcgcc
     8041 aaggcgatcc tgcagtcgga tggcgccaat cagctcattc ttttctacgg caacaccagc
     8101 agcgagcgca cgatgtgtct cgaggaactg ctggcactga aggatcagta cgtccagcgc
     8161 ctggcgctgc atttcgtgat gagccgcgag ccgcaggagg tcgagctcta caatggaagg
     8221 ctcgacggcg agcgcgtgcg gctgtttgcc cgcctcctgt tcaaacccga ggagattgcc
     8281 gaatacttca tctgcggtcc cggggacatg atcgaccagc tgggtgcgac gctgcgcgat
     8341 ctgggcgttg gcgccgagca cctccacggc gaacatttca cgctcggggc gggcgttgcg
     8401 ccacgagcgg ctgcaccggc tgccgcaccg gcggcaccgg cgagccggga cagccggacc
     8461 tcgaccgcgg tgggagccgc cgagtccatc gacggcaggg gatcgcagcc ggcctccgca
     8521 gccgatactg ccgaagtcac cgtcctcatg gatggcaggc gacggacttt cacgatgaag
     8581 atgaacgagg acacggtgct ggatgcggcc gctcgtgccg gactcgagtt acccttttcg
     8641 tgtcgggcgg gtgtgtgctc gacttgccgc accaaggtcc tgagcggcaa ggtggaaatg
     8701 gcgcagaact acgccctcga ggactgggag ctcgagcagg gctacattct cgcgtgccag
     8761 tcgcgggtga tgacgcccgt cctggagctc gattacgacg agaagtgaag cgcacgcggc
     8821 ttccgatcgt gcaaggcgag tgacctcatg aactatcaga ccattctttt cgagacgacc
     8881 gcgggcgtcg cccgcctcac cctgaaccgg cccgatcgcc tcaacagctt caatagcgcg
     8941 atgcaccagg aggtgcgcga tgccatcgcg agtctgatcg gcgattccgg ggcgcgcgtg
     9001 ctcgtcatca ccggctccgg ccggggattt tgcgccggcc aggatctcgg tgaccgggca
     9061 gtggccccgg gcggagcagc ggccgatctc ggcgagtcga tcgagaagaa ctacaagcca
     9121 ctggtgctgg cgctgcattc gttgcagatt ccggtgatcg ccgccgtcaa cggcgttgcg
     9181 gccggggcgg gcgccaacat cgcactcgca tgcgacctcg tgattgcagc gcgctccgcg
     9241 agtttcgtgc aggcgttctg caagctcggt ctcgtgccgg actccggcgg gacctggttc
     9301 ctgccgcggc tgctcggtac ggcgcgcgcc atgggattga gcctcctcgg ggacaagctt
     9361 cctgcccagc aggcggccga gtggggtctc atctggcgtt gtgtcgagga tgccgagctg
     9421 cgcgcagtgg tggatgagct cgccgcacag ctggccgctg ctccgacccg gggtctcgcc
     9481 aggaccaaac aggcgttgta tgagagctgg ggacgcaccc tcgagcagca gctcgatgtg
     9541 gaacgggatt accagcgcga gctcgggcgc acgacggatt acgccgaagg cgtcgccgcc
     9601 ttcacggcca agcgtgcacc gaagttcacg ggccgttgag gatattgcat ggccgatacc
     9661 aacgatcctg cgcagcagct cgctgaacgc gccgcccgtg cgatgtttgc tcgcgaccgg
     9721 gcggcccgga tgctcggaat gcgggtgagc cgcgtcggac ccgggcatgc cgaggtgctg
     9781 atgcaggtgg tgccggagat gatcaacgga cacgacgtct gtcacggcgg actcgtgttc
     9841 tcactcgcgg atacggcatt cgcctatgca tgcaactcgt acaacgaaag tacggtcgcg
     9901 gccgccgggt ccatcgactt cctcgcgccc gcgcgctccg gcgatgcgct gacggctgtg
     9961 gcgcaggagc tgtggcgaac ccgccgcagc ggcttgtacg aaatcacggt gaccaaccag
    10021 cgcggtgagc gcatcgcctt gtttcgcggg cgctcgcacc gcatctccgg gaaagtcgcg
    10081 ccgcaggaat cgagtgagac ccaatgagag acgcaccgag gcaactgcag agctacgcaa
    10141 gtgggcaatg ggtcgccggg tcgcacgatg ccgtcgcact gcgggatgcg accacaggcg
    10201 ctgtgattgc cagctcctcg gccagtggcc tcgattatcg cggcatgctg gagcacgcgc
    10261 gtcaggtggg cggtcccaac ctgcgccggc tcactttcca cgagcgggcc ggcctgatca
    10321 aggagctcgc gaagcggctg ggagagctca aggaggaatt ctacaagctg tcgtttgcca
    10381 ccggctcgac ccgtagcgac tcgtggatcg atatcgacgg tggcatcagc acgctgttcg
    10441 tctacgcaag caaagggacg cgtgagcttc ccaatagctg cgtatatgtg gatgggggcg
    10501 tcgaagcgct ctcgcggggt ggcaccttcg tcggccaaca catctgtgtt ccgctcgagg
    10561 gcgcggcggt gcaaatcaac gccttcaact tcccggtctg gggcatgctg gagaagcttg
    10621 cgcccgcact cctggcgggt gtgccctcca tcgtcaaacc ggcaaccgcg acctcgtatc
    10681 tcaccgagca ggtggtgcgg cgcatcgtcg agaccgggct gctccccgag ggggcgattc
    10741 agctgatctg cggcggaacc ggggatctgc tcgagcatct gagctgccag gatgtcgtct
    10801 gcttcaccgg ttctgcggcc accgcccaga agctcagaca gcacccggcg atcatggcgc
    10861 acgccgtgcg cttcacctcg gagaccgatt cgctcaactc atccattctc gggccggatg
    10921 ctgcggccgg caccccggag ctggagctgt tcgtgcgcga agtgattcgc gagatgacgg
    10981 tcaaggctgg ccagaagtgc acggcgattc gcaaggccat cgttccgcgg gagcgggtgg
    11041 cggatgtcgt ggacgccatg aaggctgcac tcggccaggt gatcgtcgga gatccccgcc
    11101 tcgagcaggt caagatgggc ccgctggcct cgctcggcca gcgccgcgaa gtgcgcgagc
    11161 agctcgcgag actgcagcgc gaggccgagc tggtttacgg cgcgcaggag accgagccgg
    11221 tcggggccga tggcgagcgc ggcgcatttc tatcgcccaa gctgctgtac tgccgcgaca
    11281 gtgcctcggc gcgcgaggtc cactcggtcg aagcgttcgg gccggtgtgc acgatcctcg
    11341 gctacgacac tctcgatacg gcgatcgcgc tcgctcgtcg aggcggcgga agtctggcgg
    11401 catcggtgtt cagcgccgat gagcaggccg ctgcccgact cgtgctgggg cttgcccctt
    11461 ttcatggccg agtgctcgtc gtcaaccgtc actgtgcgaa ggagtccacc ggacacggct
    11521 caccgctccc gcatctggtg catggcggcc cgggccgagc gggtggcggc gaagagctgg
    11581 gcggcatccg cggcgtgctg cacttcatgc aacgaaccgc cgttcagggc actccggatg
    11641 tcctcaccgc cgtgacgggc cgatgggcga aaggtgcccg gcagatcgat ccaggcgtgc
    11701 atccgtttcg taagccattt ggtgcactcg tcatcgggga tacgttcaac tcgggcgagc
    11761 gcgaggtgac gatcgaggac atcgagcggt tcgccgagct ctcgggtgat cgcttctatg
    11821 cgcacatgga cgaggaggcc gcgcggcgca atccgctgtt cggcgggcga gtcgctcacg
    11881 ggtacttcct ggtctcggcc gccgccggcc tgttcgtcga tccgccttac ggaccggtgc
    11941 tcgccaacta cggtatcgat gggttgcgct tcaccaagcc cgtcaagccc ggcgaccgca
    12001 tcaaggtccg cctcacttgc aaggagaagt ccttacgcct cggaatgggc tatggcgagg
    12061 tccggtggga tacgcaaatc agcaatcaga acggggaagt cgtcgccagc tacgatgtcc
    12121 tgacgatggt gagcgaaaag agcgttccag acgcatgaag cgcgtaacgg acaaggccag
    12181 gccggcaaag cgcccccgaa gcgccgcgac cggcagcgcg cccgccgagg agcgcgtcaa
    12241 caccgtggtc gagcggtatc gatcgcgctt tccagctctg cagttttcgc agcctgcggc
    12301 ggcggtactc gaagtcgtgc tcgccaaccc ggcccgtctc aactccgccg atgcgagcat
    12361 gcaccgggag ctggcgagcg tgtggcgcga gattgatgcg gatgaggcag tccgggcagt
    12421 cctcgtgcgc ggggagggcg ctcacttctc ctcgggcggg gatttctcgc tgatcgagag
    12481 catgatcgag gacgaggcca ccctcgtgcg cgtctggaaa gaggcgagcg atctggtcta
    12541 caacctcatc aactgctcca agcccgtggt atcggcaatc cggggcaccg cggtcggagc
    12601 cggactggcg gtcgcgctgc tggcggacat ctccattgcc gggcgcgacg tgaagatact
    12661 cgacgggcat acccggctcg gcgttgctgc cggtgatcat gccgtcatca tctggccgct
    12721 gctctgtggg ctggccaaag cacgttatta cctgctcacc aacgagccgc tctcgggcga
    12781 ggaggcggag cgcattggtc tggtcgcgcg ctgcgtggcg gacgaagagg tctacccgac
    12841 agcactccag gtcgctgcca ggctggccca gggcagcagc acggcgatgc gctggaccaa
    12901 acacgcactg aacaactggc tgcgtctggc gggccccagc ttcgacacct cgctcgcgct
    12961 ggaattcctg ggtttccggc tccacgatgc gcgcgcaggt ctggaagcgg tcaggaatcg
    13021 cccgaagcgc cgctgagccg gaacgcatgg ccgcgggtgg atacggcctt gaccaacacc
    13081 cagatgctca tccccggacg caacgccaac tccaccgctg cacttcgggt gattcgggcc
    13141 agcacgatcg tggcggcgcc gatgtccacc ttgaccagca cggtttcgtg atcgtcgtcc
    13201 agcagctcca cgatcgtgcc ctggagcgcg ttgcgcacgc tcagaccttt gggctcctgc
    13261 agacccagga tgatgtcgcg cgcgagcaga tggacgcgca cgcgtgtgcc tgctgtgacc
    13321 tcacgcacac tgacgtgcag cttgccgcgc cccacttgta gctccgccat tctttgcgtc
    13381 ccatcgaagc gtgtcacctg gccgtccacg accgcgccta cggcatcggg cccgacaagc
    13441 gagcgcagcg gcggcagcag gctgatttcg ttcaccgttc cctgccccgc aacgcgccct
    13501 ccttcgagca gaacgacgta ggtcgccaga cgcagcacct cgtcgagctg atgactgaca
    13561 tacaccatgg ggatcgacag gctgtcgcgc agcagctcca gatagggcag cacctcctcg
    13621 cgccgcgcga catcgagcga ggcgaggggc tcatcgagaa gtaacaggcg cggttgcgag
    13681 agcagggcgc gacccagaga ggcgcgctgg cgttcgccac cggagagctg atgagggcgg
    13741 cgccgcagaa gtggcttgag gccgagcagg gcgacgaccc gctccagctc gatcggctgc
    13801 gcggcaggcg cggcgcgctt ctggccgtat tgaagattcc cgagcacgct caaatgagga
    13861 aacagtctcg gctcctggaa aacgtagccg atgcagcgcc gctcggccgc aatgcgcatc
    13921 cccgtagccg tatcctcgag caccgacccg ttcagctcga cgcggcccgc atcgggggcc
    13981 agcagtccgg cgaggatatt gactgtcgtg gtcttaccgc tgcccgagcg gccgaacaac
    14041 gcaacgaccc caggggtcgg cgcctcgaat tgcgcacgca gcgtgaagct gccgaggcgc
    14101 ttgagcacgg cgaccttcag catgcgtgcg gcgcgagcac tcagcggccc agctttctgc
    14161 ggacacctct ggccaggact tcggccagca tcagtccgag caacccgagc gcgaaagaca
    14221 ggatggcgag ccgcagcgcc tgtgcatcgc cggtgggcga ttggatcgcc gtgtagagcg
    14281 cgagcggaat ggttcgtgtc tcgccgggga cgttggccgc aaaagtgatc accgctccga
    14341 actcgccgag tccggcggca aatgcggtca cggcgcctgc gatgacgccg gggagcatga
    14401 gcggcaaggt gatcgtgcag aatcgatcga gagggcctgc accgagggtc tgggctgcct
    14461 cctcgaaccc gcgatcgatg ctctcgagcg agatgcggat cgagcgcacc atgagcggaa
    14521 agctcatgac agcggtggcc agcgccgcac cagcggtcgt gaacgcaagc tgcacgccga
    14581 aatgcgcatg cagccacccc ccgagcggtc cccgattccc gaacagcaac agcagcacat
    14641 agccgactgc gacagggggc agcacgaggg gtagatgcac gaaggcatcc aggacggagc
    14701 gtcccgggaa tcgcccgcgg gagagcaccc aggcgatcag gagcgcccag ggaagactga
    14761 acagggcgct gcacaaagcc acctcgaggc tcaggcgcag ggcctcccat tcggcggggc
    14821 tgaggatctc gtgcatgggc cggacgcaga cgtagccttc tgttcagggt gactttcgca
    14881 tgccgcccgg ctggtcggcc agaggcttga ctccggcccg gccgcccaca tttctgtggc
    14941 acgccccttg cgggcgagcc ctaccctcat acggaaccct tccctcagat gagcatcgat
    15001 cggcagttcg gctggatgtg ggcgcaggcg tgcgagttgc tcgaccaggc ggatcgcatg
    15061 cagcgccagt tcgtccggta tgtgggacag gatgcggagg cggccgtctg ggagccgccc
    15121 gtggacatcc aggagagcgc cgaaggcgtg catttcactt ttgcgctacc gggtgtggat
    15181 cccgatcgga tcaatgtcag tctcgatccc accgcgctca cggtgagcgc cgtccgagtg
    15241 ccgcggctgg gcggctcggg ctcggtggtg cgacgcctgg aaatccccca cggtcgcttt
    15301 gtgcgacgca tcgcattggc tgccgcgcgt ctgcggctcg gagagagccg gtattcaaac
    15361 ggctgtctgg aagtccgcct ggtgtggacc gacgacaatc gaggatgcaa ataacatggc
    15421 cgatacagaa aaccccgctt cgacggcgga gccgacttcg ccaccgagcg atgccatccc
    15481 cgtcatcgcc atgcgcaccg tggtcgtatt ccccggggtc ctcgcgcccg tggccctcgg
    15541 ccgtgaggaa agcattgccg cagcacagga ggccgtgcgc cacggccgca agatcggact
    15601 gctgttgcag caggatccgg ctgtcgagaa gcccaccgct catgatctac acccgatcgg
    15661 agtgctggcc tcggtcgtcc ggtacgtgac tgcccccgat ggcacccatc atctggtcgc
    15721 tcagggtgag agccgtttcc gcgccgtgga gttcgtgcag gagaggccgt acctggcggc
    15781 ccgcacggag gtccttgcgc cggccaacac caccggcccg gaaatcgagg cgcgtgtgga
    15841 ggttctcaag cagcgggcca tcgaggccct gtccctgctg ccgcaggtcc cgaccgagct
    15901 cacccggacc attcagtcga tcgactccgc gagccagctc tccgacctga tcgtcagctt
    15961 catggacgtt aaggtgcagg acaaacagga gatgctcgag acactcgacc tgcgcgagcg
    16021 gctggacaag gtcctgtcgc tcctctcgaa ccggctgcag gttcttaaga tcacgcgcga
    16081 catcaccgag cagacgcaga gcgcgctcgg cgagcgccag cgtgaggcgg tgctgcgcga
    16141 gcagatgcga cagctgcaga gggagctcgg cgaatcggag gacagtggca gcgaggtcgc
    16201 cgagctcgag aaggccgtgg agcgcgccgg catgcccgcg gaggtggccg agcatacgcg
    16261 caaggagctc aagcgactgc aacgcatgag cgaggcgagc ccggagtacg gcatggtccg
    16321 cagctacctg gatactctgg tcgcgctccc gtggagcacg gccgatgcgg aagacatcga
    16381 cattgagcgc gcccgtcgca tcctggacga ggaccattac ggcctcgaca aggtgaagcg
    16441 ccgcatcctc gagtacctcg cggtgcgcaa gctgaatccg caggggcgca gtccaatctt
    16501 gtgcttcgtg gggccgccgg gggtggg
//
LOCUS       DASRHN010000054        14916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_349418, whole genome shotgun sequence.
ACCESSION   DASRHN010000054 DASRHN010000000
VERSION     DASRHN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349418"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..595)
                     /locus_tag="VFB37_02075"
     CDS             complement(188..595)
                     /locus_tag="VFB37_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21262.1"
                     /translation="MSATVLEECAELGRFMRGETDPACFPHREHVRMGFEMLRRHDFA
                     EAAFRYSDALRSIVTKAGKAKIFHQTITIAFLSLIAERMEAAPYPDFETFAIANGDLI
                     DKSALRRCYRAERLASEAARRTFLLPEPIQRTP"
     gene            complement(576..827)
                     /locus_tag="VFB37_02080"
     CDS             complement(576..827)
                     /locus_tag="VFB37_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21263.1"
                     /translation="MKEITRTQTGVRLETQLLKVLKALATELDLSLGDLLEGIVLHAF
                     EGKTPFSAPTLKKIRTLRSVFGLELTAADSHRLHERDSA"
     gene            complement(966..3017)
                     /locus_tag="VFB37_02085"
     CDS             complement(966..3017)
                     /locus_tag="VFB37_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO21264.1"
                     /translation="MKNLLVTALVLGVVTAPGAVRADQPPIIDRNLFFGEITIGGAQI
                     SPDGKTLSFLKPFKGTRNIWVKKAAEPFSAARPVSAETARPVRVYFWSRDSKYLLYVQ
                     DVGGDENFNVYAIDPSLPADPKTGVPPTRALTNLKGVRTELFAAPKAKPDILYIGLNN
                     RDAHWHDLYELHISSGKRTLLRKNTEQIAGWDFDNDGNLRLAERTNNAGDTEILRVDA
                     DGFRQIYSCSVLESCGVEGFDASNHSSWLITNKGNLDLAELDSMDPQTGATAKVESDP
                     ENRVDLTGVTTSEVDHHILFTEYEDSVPRRYFRDKAFEKEWRWLSAHLGGRQITLGSH
                     SQDENLWVLTAYSDTDPGSTYVWNRKAKSVTLQYRIREELPRGSLSERRPYEFKSSDG
                     LVIPAYLTLPKGLTPKELPLVVWPHGGPWYRDTYGYDTFAQFLANRGYAVLQPNFRGS
                     TGYGKKFLNAGNGEWGRKMQDDLTWGAKALIGAGIVDAKRVGIGGGSYGGYATLAGVA
                     FTPDVYAAAVAIVAPSNLITLLDAIPPYWEAGRKQMYTRMADPTTPQGKALLVAESPL
                     TRAKSIVTPLMVVQGKNDPRVNIRESNQIVAAVRDNGKPIEYLVAPDEGHGFARPINN
                     LAMVTAMEKFYAHYLGGRTQTEVPAEVAARLAEITVDPKTVNGAVTLKGALAKPAGSG
                     E"
     gene            3144..3953
                     /locus_tag="VFB37_02090"
     CDS             3144..3953
                     /locus_tag="VFB37_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21265.1"
                     /translation="MRATLFLLTFLGLASSGLSAAAAPDPALEHAVADPGRSQKFVSR
                     DRYRHPVEELTFFGLKPTMTVVEVWPGSGYWTEILGPYLKTSGHYYAAVTVPKESQEE
                     DRTTAAWRTAVTGDKARYGDIVITQLGKGHYEVAPAGSADLIVTFRNLHNWMEGGYVD
                     EALKGFYTALKPGGVLGIEDHRGRNDQPQDPQAKSGYVRQDYVIEHARKAGFELVATS
                     EIDANPKDTKNWPEGVWTLPPTLALGDKDRQKYLAIGEADNFVLKFRKPAH"
     gene            complement(4001..4978)
                     /locus_tag="VFB37_02095"
     CDS             complement(4001..4978)
                     /locus_tag="VFB37_02095"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZO21266.1"
                     /translation="MQHEFAILGAGAIGSILGAHLARAGHDVVMLARGRRAEQIEAQG
                     LRITGLSNFGVPIAVLREPARLRASGTLIVAMKTPATEDALDRVRHIEVDVAFSIQNG
                     PLKDDLLVTTFGSGRVLGALADTSGELKPDGEVAFTRNVNVLLGELSGETGERAHRIA
                     QVLDAAGIRALAVPHIRSLEWSKFAAWVGLFALSITTRAVTWRYLADPGAALVLVRLV
                     RETGLLAGRLGIALSDQAILPVASLCEGTEQSAVEKVLQAGKAFAILAPDHRMSSLQD
                     LQSGRPLELHETLGYTLKRAAAHGLSLPLIEAFYSVIAAAEKFRESAPR"
     gene            5714..6004
                     /locus_tag="VFB37_02100"
     CDS             5714..6004
                     /locus_tag="VFB37_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZO21267.1"
                     /translation="MTKLYVGNLPFSATEDGVRALFAAHGTVEKVSLITDRDTGRPRG
                     FGFVEMSNADASRAMQALNGKDFEGRPLKVNEAQDRDRAGGGNRGGGGGRRY"
     gene            complement(6094..6348)
                     /gene="minE"
                     /locus_tag="VFB37_02105"
     CDS             complement(6094..6348)
                     /gene="minE"
                     /locus_tag="VFB37_02105"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HZO21268.1"
                     /translation="MGLLALFRRTPPSANVAKERLRIIVAQERSARGGPDYMPLLRRE
                     LLEVIRKYVNVDPGAVQVNVEREHGQDILELSVALPERAR"
     gene            complement(6350..7162)
                     /gene="minD"
                     /locus_tag="VFB37_02110"
     CDS             complement(6350..7162)
                     /gene="minD"
                     /locus_tag="VFB37_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HZO21269.1"
                     /translation="MSEIIVVTSGKGGVGKTTTSASIACGLARRGKRVAVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGECTLRQALIKDKRIETLFVLAASQTRDKEALSADGV
                     RRVFDELAADGFDYIICDSPAGIEKGAHLAMYFADRAVVVVNPEVSSVRDSDRILGLL
                     ASKTHRAEQGNGGVREHLLLTRYSPKRVATGEMMSVADVKEILGLDVVGVVPESTDVL
                     SASNAGIPVILNPESDVSRAYEDAVARLLGEMVPLRFVEEPKKGFFARMFGG"
     gene            complement(7287..8018)
                     /gene="minC"
                     /locus_tag="VFB37_02115"
     CDS             complement(7287..8018)
                     /gene="minC"
                     /locus_tag="VFB37_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HZO21270.1"
                     /translation="MNTPADAARPQAIEIRFGQVGLVQLRLHTVDPGAILDELTGRTA
                     TAPHFFERTAVCLDLSALGPAPEAHQVRAVVDVIRRAGLLSVGLAQGPASAEALAREL
                     DVPVLTHIRIPNRPVPVVQAAPEVALQPAQMQHQPVRSGQRIYARNRDLIVTTTVAAG
                     AEVMADGCVHIYGPLRGRVMAGVHGETSARMFCQEFHAELVSIAGIFRVFETIPPELA
                     GAPVQAWLAGEELHVARIGAGAAQT"
     gene            complement(8059..8811)
                     /locus_tag="VFB37_02120"
     CDS             complement(8059..8811)
                     /locus_tag="VFB37_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZO21271.1"
                     /translation="MPNQASWLGRVPVAVENAQGRGPLVLVCDHASNWVPPALARLGL
                     APQELDRHIAWDPGALPVAQSLSRLLDAPLVHAGISRLVLDVNRDPDHAGSIVSLSED
                     TRIPGNVAITAEDRMRRIRDIYEPYHHCLERTVDTQVARHADLQVVSIHSFTPIYRGH
                     HRPWHVGVLSDRDRRMAEPLLRLLQADPDMNVGDNEPYAPTDGVYHTLGRHCSVRGLR
                     SVLLELRADLVGDAASQERWARKLAQALRAIS"
     gene            8895..10445
                     /locus_tag="VFB37_02125"
     CDS             8895..10445
                     /locus_tag="VFB37_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HZO21272.1"
                     /translation="MIRAHALYTLLAAAVLLPGCHGGDQGNAPGTSGPPPRGTLLIRP
                     PPMLASYSTADLLKSLGGNDLGKELLTLAYSPICTVNVYQLEFETVGGQGEATKSSGA
                     LMIPVGLNSACNGPKPIVLYAHGTTADKTFNIAALNQSNDAEGLLLAAVFAAQGYIVV
                     APNYAGYDTSDLPYHPFLDADQQSKEMIDVLSAARDALPIASAPGVSDNHKLLLTGYS
                     QGGFVAMAAQKAMEAAGVTVTAAAPMSGPYAPAAFADAVFMGQVNLSATLNVTLLVSS
                     YQHVYGNIYQQPTDLFSAAYAGDIESLLPSTTPISELYSERKLPESELFSNVPPAPEF
                     ASLTPATTPAALAPAFALGFGPDALITNDYRLAYLRDEQANPDGGFPTVSTGVPAETP
                     ANTFRQDLKLNDLRSWTPGAPVLLCAGDQDPTVFYLNTSLMQRYWMMNAPGAVVTVLD
                     VDGPVTSGDPYATLKKAFGAAKALTAADAVLGGAKDGGAMAVLQAYHAGLVPPFCLSA
                     VKSFFDGF"
     gene            10458..10679
                     /locus_tag="VFB37_02130"
     CDS             10458..10679
                     /locus_tag="VFB37_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21273.1"
                     /translation="MATKLDKQLKREIDIDGKPYMVTLSPEGVKLTEKGKRKGQELSW
                     KGLVNGQAATAVSLAGSGPEAGQSGSEMG"
     gene            complement(10697..11164)
                     /locus_tag="VFB37_02135"
     CDS             complement(10697..11164)
                     /locus_tag="VFB37_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO21274.1"
                     /translation="MDKIDHRIITLLEAEARQSFADLGQQIGLSKTPCWQRVRDLERA
                     GVIKGYRAEIDAAQLGLHVHAFVHVTISSARHDDFESAVQRHPAILQCFTTAGQADYL
                     MHVLVAGISELDALLRREISRMPGVQRIETTVCMKTIKQRAPVTGCLPATAGA"
     gene            11388..13067
                     /locus_tag="VFB37_02140"
     CDS             11388..13067
                     /locus_tag="VFB37_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21275.1"
                     /translation="MATPAQYAQNQQASGIDRAHRSVIFASSLGTVFEWYDFYLYGSL
                     SAVISKQFFSGVNETSGFIFALLAFAAGFFVRPFGALVFGRVGDLVGRKYTFLITIVI
                     MGTSTALVGVLPSYASIGVAAPAILISLRLLQGLALGGEYGGAATYVAEHAPAGRRGL
                     YTSWIQTTATLGLFLSLLVILVCRVSLGAQFESWGWRIPFLASIVLLAISVYIRLQLR
                     ESPAFLEIKSQGKLSKAPLTEAFGQWSNLKIVLLALFGATAGQAVVWYGGQFYALFFL
                     EKILQVDSKAANLIIAAALLIGTPFFVVFGSLSDRMGRKTIVMAGCVLAVFTYFPVFK
                     GLTHYANPALEAAVATSPVTVIADPGDCSFQFDPVGKKTFTRSCDVAKAALAKAGVPY
                     ANEAAPASSVARLRIGTGAQAVFVDSFAGNTLAKPDFKTANEAFSKQLKGALAQAGYP
                     PKADMSRLDYPMVILLCAYLVVLVTMVYGPIAAWLVELFPTRIRYSSMSLPYHIGNGW
                     FGGFLPVTSFAIVAATGGIYNGLWYPVIVAAATAIIGTLFLRETRGTSLAT"
     gene            13085..13357
                     /locus_tag="VFB37_02145"
     CDS             13085..13357
                     /locus_tag="VFB37_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HZO21276.1"
                     /translation="MSEEGMRELLVKVHERLRTSGSLDADARELLLTVMRDIEAALGA
                     ADAGASRTRVSHVPRLEALAVRFEAGHPGLAQVLRQLATVLGQAGL"
     gene            complement(13360..>14916)
                     /gene="rpoD"
                     /locus_tag="VFB37_02150"
     CDS             complement(13360..>14916)
                     /gene="rpoD"
                     /locus_tag="VFB37_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZO21277.1"
                     /translation="VRMYMREMGTVELLTREGEIRIAKRIEEGLDAVRSALALYPATY
                     DFLLKAYEPVKAGQARLVDVIVGFIDPNAPDVIAQPQNPTKMELVEADEKSDDEEEGE
                     DASDSEEEAVDTGPDPEEAATRFASIGKGYIHALSAIEKVGARDPKSLKTRKKLADEF
                     MELKLSPRMFDALILNLRTHVAEVRQLEKEIMIIAVRDAGMPRKDFISTFPKNETSSR
                     WLARHVKSGKKYSAPLAKFKDEIERRQKKLEQLETQYHLTINDIKEVNRDVSIGEAKA
                     RRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTY
                     ATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAVRME
                     MPEDKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTSVASPIDQATMESLRETTHS
                     VLAQLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRS
                     EQLRSFLMDD"
BASE COUNT         2557 a         4764 c         4761 g         2834 t
ORIGIN      
        1 ccgtcgagcg gaaagatatt ctttggggcc atgctgcggt gtgccgtggg tgagagcgcg
       61 aactcgcgca gtgtactacc tggctttccg aaccttcacg ccatccattg cgtatcgtgc
      121 gctcgggcac ctgcgggtgc cgtcgcatcc cgccacgtcg ggggcttgtt tccggggtcg
      181 gacgatccta gggtgtccgc tggatgggct cgggaagcag gaatgtgcgt cgcgccgcct
      241 cgcttgccag cctctccgcg cggtagcagc gcctcaaggc cgacttgtcg atgagatcgc
      301 cattggcgat cgcaaacgtt tcgaaatcgg ggtagggggc agcctccatc cgctcggcga
      361 tgagcgacag gaatgcgatc gtgatcgtct ggtggaagat tttcgccttc ccggctttcg
      421 tcacgatcga gcgcagggca tccgaatatc ggaaagcggc ctctgcgaag tcatgccggc
      481 gcagcatctc gaaacccatc cgcacgtgtt cccgatgcgg gaaacaggca gggtcggtct
      541 ccccccgcat gaagcgtccg agctctgcgc actcctcaag cactgtcgcg ctcatgcagc
      601 ctgtgcgagt cggctgccgt cagctcgagg ccaaacaccg acctcagtgt gcggatcttc
      661 ttcagggttg gcgcgctgaa gggtgtcttg ccctcgaagg cgtgcaggac gattccctcg
      721 agcaggtctc cgagcgacag gtcgagctcg gttgcgagtg ccttgagcac cttcaacagt
      781 tgagtctcga gccgaacgcc ggtctgggtg cgggtgattt ccttcatggt actatggtac
      841 caagttacca cgaatccggc aagcatcgca cgcgacgccc ggcaaacact gcacggggct
      901 gtgagcctgg tgcagctggc accaggcggg ggcgcgcagc ggctatctcg gccgctgcgc
      961 tgtcctcatt ctcctgaccc ggccggtttc gccagcgcgc ccttgagggt gacggccccg
     1021 ttcacggtct ttggatcgac agtgatctcc gccagccggg cggcgacctc agcgggcact
     1081 tcggtctgtg tgcgcccgcc cagatagtgt gcgtagaact tctccatcgc tgtgaccatg
     1141 gcgaggttgt tgatcggtct ggcaaaccca tggccttcat ctggagcgac caggtattcg
     1201 atcggtttgc cgttgtcgcg cacggcggcg acgatctggt tgctttcacg gatgttgacc
     1261 cgcggatcgt tctttccctg cacgaccatc aacggggtga cgatggactt ggcgcgtgtc
     1321 aggggcgact ccgccacgag cagcgctttg ccctgcgggg tggtcgggtc ggccatgcgt
     1381 gtatacatct gcttgcggcc ggcttcccaa tacgggggaa tggcatccag aagcgtgatc
     1441 aggttcgacg gcgcaacgat tgccaccgct gcggcgtaca catccggtgt gaaggccact
     1501 ccagccagtg ttgcgtagcc gccgtaggag cccccgccga taccgacccg cttcgcatcg
     1561 acgatgccgg ctccgatcaa cgccttggct ccccacgtca ggtcatcctg catcttgcga
     1621 ccccactcgc cgtttccggc gttgaggaat ttcttgccgt accccgtgga tccacggaag
     1681 ttgggctgca ggaccgcgta gccgcgattg gccagaaact gggcgaaggt gtcgtacccg
     1741 taagtgtccc ggtaccaggg tccaccgtgc ggccacacca ccagtgggag ctccttcggc
     1801 gtcaatccct tgggtagcgt caggtacgcc ggaatcacta ggccatccga tgacttgaac
     1861 tcgtacgggc gccgttcgga caacgacccg cgcggcagtt cctcgcggat ccggtactgc
     1921 agtgtcacgg actttgcttt gcggttccat acgtacgtgg aacccgggtc ggtatcgctg
     1981 tatgcagtca gcacccacaa attctcatcc tgtgagtgtg agccgagggt aatctgccgg
     2041 ccgccgaggt gagcgctcag ccagcgccac tccttttcaa aggccttgtc gcggaaatac
     2101 cggcgcggga cgctgtcttc gtattcggtg aacagaatgt ggtggtctac ctcggaggtc
     2161 gtgacgccgg tcaggtcaac ccggttctcg ggatcgctct cgactttcgc cgtagcgccg
     2221 gtctggggat ccatgctgtc gagctcggcc agatcgaggt tgcccttgtt ggtgatgagc
     2281 cagctgctgt ggttcgaggc gtcgaagcct tccacgccac aggattccag caccgagcaa
     2341 ctgtagatct gcctgaagcc gtccgcgtcg actcgcagta tttccgtgtc gcccgcgttg
     2401 ttggtccgct cggccagtcg gaggttgccg tcattgtcga aatcccaacc ggcaatctgc
     2461 tccgtgttct ttcgcaacag agttcgcttc ccgctcgaga tgtgcagctc atacagatcg
     2521 tgccagtgcg catcacgatt gttcaggccg atatagagaa tgtccggctt ggccttggga
     2581 gccgcgaaga gctcggtacg cacgcccttg agattcgtca gggcgcgcgt gggcggcacg
     2641 ccggtcttcg gatccgcggg caggcttgga tcgatggcat agacattgaa gttctcgtct
     2701 ccaccgacgt cctgcacgta aaggaggtat ttcgaatcgc ggctccagaa atagacccgc
     2761 accggccgtg cggtctccgc gctcacgggc cgcgcggcgc tgaaaggctc ggcggctttc
     2821 ttgacccaga tgttgcgagt ccccttgaag ggcttgagga atgagagagt cttgccgtcc
     2881 ggggagatct gcgcgccgcc gatcgtgatc tcaccgaaga acaggttgcg gtcgatgatc
     2941 ggcggctgat cggcgcgcac cgcaccgggt gcagtcacga cccccagcac cagggcggtc
     3001 acaagcaggt tcttcatggg agcctcggga gacgtcagag ctcgattgga tcggcgcatt
     3061 gtgccagtga aggggcccgt ttgcccgccc ggggcgcgtg ttaagatcgc gcccccagcc
     3121 ggatcaggct cacggagcgt tccatgcgcg caactctttt tttgctgacg ttcctgggtc
     3181 ttgcctcgag tggtctgagc gcggcagcag caccggatcc tgccctcgag catgcggtgg
     3241 ccgacccggg ccgcagtcag aagttcgtca gccgcgatcg ttaccggcac cctgtcgaag
     3301 agctcacgtt tttcgggctg aagccgacca tgacggtggt agaggtctgg ccgggcagcg
     3361 gctactggac ggagatcctg gggccgtatc tcaagacatc gggccattac tatgccgcgg
     3421 tgacggtgcc gaaggagagc caggaggagg atcgcacgac ggccgcctgg cgaaccgcgg
     3481 tgactggcga taaggcacgc tatggagata tcgtcatcac acagctcgga aagggacatt
     3541 atgaggtggc tcctgccggc tcggccgatc tcatcgtgac gtttcggaac cttcacaact
     3601 ggatggaggg cggctacgtc gatgaggcct tgaagggctt ctacaccgcg ctgaaaccgg
     3661 gtggcgttct cggcatcgaa gaccaccggg gacggaatga ccagccgcag gatccgcagg
     3721 ccaaatccgg ctacgtgcgc caggactacg tgatcgagca cgcgcgcaag gcaggcttcg
     3781 agctggtcgc gacctcggag atcgatgcca atcccaagga caccaagaac tggcccgagg
     3841 gggtctggac gttgccgccc acgctcgcgc tcggcgacaa ggatcggcag aagtacctcg
     3901 cgatcggcga agcggataat ttcgtcctga aattccgcaa acccgctcac tgagggagtg
     3961 cgccgggcag cggtgcgccc gtggctgcga cagccaaagc tcagcgcggc gcagactccc
     4021 tgaacttctc tgcggccgcg atcacactgt agaacgcctc gatcagagga agcgacaggc
     4081 cgtgcgcagc ggccctcttc agggtgtatc ccagcgtctc atgaagctcg agcggccggc
     4141 ccgattgcag atcctgcagg gaagacattc tgtggtcggg tgcgagtatc gcgaacgcct
     4201 tgcctgcctg cagcaccttc tccaccgcgc tctgctccgt tccctcgcac aaggatgcaa
     4261 ccggcagaat ggcctggtcc gagagggcga tgcccaatcg acccgccagc agtcccgtct
     4321 cacgcaccag cctcaccagc accagcgccg caccggggtc cgcgagatag cgccaggtca
     4381 ccgcgcgcgt ggtgatcgag agcgcaaaca gtcccaccca cgcagcaaac ttcgaccatt
     4441 ccaggctgcg tatgtgggga actgccaggg cacggatgcc agcggcatcg aggacttgcg
     4501 cgatgcggtg cgcccgttcg ccggtttcgc ccgacaactc accgaggaga acgttgacat
     4561 tgcgcgtgaa ggcgacttcg ccgtcgggct tcaattcgcc gctcgtatcg gcgagtgcgc
     4621 cgagcacacg ccccgagccg aatgtggtga cgagcagatc gtccttcagc ggaccattct
     4681 gaatcgagaa agcgacatcc acctcgatgt gacgcacgcg gtcgagtgcg tcctccgtgg
     4741 ccggtgtctt cattgcaacg atcagcgtgc ccgaggcgcg cagccgggca ggctcgcgca
     4801 gcaccgcgat ggggaccccg aaattgctga gacccgtgat gcgcaggccc tgagcctcga
     4861 tctgctccgc ccgccggccg cgggcgagca tgaccacgtc gtgacctgcg cgtgccagat
     4921 gcgctccgag gatcgagccg atcgcaccgg cacccagtat tgcgaactca tgctgcatcg
     4981 tttcagtgtc cgagggagca catctccccg ggctctgcgg ctgcattgtg gaccaacggc
     5041 gtcgtggcgt ggggaagttg ggtcgtgcgg ggcggcgcgg atccggagct gccgatgaac
     5101 ccgcaacgcc gggatgatgt tggtcacggg cgtagagcga gagtcgttca gcgtccgatc
     5161 agcttgaata cgccatcctt ctgacgcggc cgaagcatcc gtgcacgcag gaccggcccc
     5221 accgagcgtc tcccgattgg agcgcaccgc gcagacggca ggggggcagg cccaggtgac
     5281 agactcgcgc cggcatggag ctggctcccg acgggcgcat tcaagtccgc ggattcgggc
     5341 cggcacatgc cggtgcgggt ccaatggccg ctgactgagc acgagttcca ccgtcaaccg
     5401 catgaccagg acaggcttcg ccagcggtcc agtgcacgct ttgagagtgc cgcgcttacg
     5461 ccgaggcatg gttatcgccc ctcgggggca ccccaggcgg ggtgcccgcc aacatgggat
     5521 gcggatcttc gacagcccgg gggggactgc tgtaagctgc cggccggctc gtgatctttc
     5581 ggcttgtctg tcttcggccc ggcgcccgcg cggcgctcct tccggccaaa gtctgccacc
     5641 ctcggaaagc ttggcgaacc aggaggcagg ttcccctgcc gagtttcttc ttttctctgt
     5701 gagtatttca gcagtgacca aattatacgt tggaaatctt cctttctcgg cgaccgaaga
     5761 cggcgtgcgg gcgctatttg cagcgcatgg caccgtcgag aaggtatccc tcatcacgga
     5821 tcgcgatacc ggccgtccgc gtggcttcgg tttcgtggag atgtcgaatg cggatgcttc
     5881 ccgggccatg caggccctga acggcaagga cttcgaaggc cggccattga aggtgaacga
     5941 agctcaggat cgcgatcggg ccggcggcgg caatcgcggc ggcggcggcg gacgccgtta
     6001 ctgagtaaga atcccgcctg aacagtcccc gtgcgccgca tgcgtggcgc gcggggacgt
     6061 ctttaagcct ccctgcgagg ccttcaattc ccttcagcga gccctctcgg gcagcgccac
     6121 ggacagctcg agaatgtcct gaccgtgttc gcgttcgacg ttgacctgga ccgcaccagg
     6181 atccacgttg acgtacttac gaatcacttc cagcagctcg cggcgcagca gcggcatgta
     6241 gtccgggccc ccgcgcgcac tgcgctcctg ggcaacgatg atgcgcaggc gctccttggc
     6301 aacgttcgcc gaaggaggcg tgcgacggaa aagagcgagc aaccccacgt cagcctccaa
     6361 acatgcgagc gaagaagccc ttctttggct cttcgacgaa cctcagcggc accatttcgc
     6421 ccagcaggcg cgccacggca tcttcgtacg ctcgcgagac gtcgctttcg gggttgagga
     6481 tgactggaat gccggcattg gatgctgaaa ggacatcggt cgactcaggc acaacgccaa
     6541 ccacatcgag gcccagtatc tccttcacgt cggcgacgct catcatctct ccggtcgcga
     6601 cccgcttcgg gctgtaccgg gtcagcagga gatgctcccg gacaccccca ttgccctgct
     6661 cggcgcggtg agtcttgctc gcaagcagcc cgaggatccg gtccgagtcg cgaaccgagg
     6721 agacttccgg attgaccacg accacggccc gatcggcgaa atacatcgcc aggtgcgctc
     6781 ccttctcgat ccccgccggt gagtcgcaga tgatgtagtc gaagccgtcc gcagccagct
     6841 cgtcgaacac gcggcgaacc ccgtctgcgg agagggcctc cttgtcgcgg gtctgcgagg
     6901 ccgcgagcac gaacagtgtc tcgatgcgct tgtccttgat gagcgcctgg cgcagggtgc
     6961 attcgccctg gatcacgttc acgaaatcgt aaacgacccg ccgctcgcac cccatgatga
     7021 ggtccaggtt gcgcagaccg atgtcgaagt cgatcacggc gactcgcttg ccccgccgcg
     7081 cgaggccaca ggcaatgctg gcggaggtgg tcgtcttgcc gacgccgccc ttgccggagg
     7141 tgacaacgat gatctcgctc aatgttggat ctccttgggt cagatcggcg cgtggcgtcg
     7201 cgccaggatt actggatggg tctgggcacg agggggcggc cgcattcaga tccacggccc
     7261 agcctccgat cgcgcgcgcc ccgtcatcag gtctgggcag cgccggcgcc gatacgcgcc
     7321 acgtgcagct cctcgccggc gagccaggcc tgcaccgggg cgccggcgag ctcgggcggg
     7381 atcgtctcga agacacgaaa gatgccggcg atcgagacga gctccgcgtg gaactcctga
     7441 cagaacatgc gcgcgctcgt ctcgccgtga actcccgcca tgacgcggcc gcgtaggggg
     7501 ccatagatgt ggacgcagcc gtccgccatc acctcggctc cggctgccac tgtggtcgtg
     7561 acgatcaggt ctcgattgcg ggcgtaaatg cgctgtccgg agcgcacggg ttggtgctgc
     7621 atctgcgcag gttgcagcgc cacctcgggt gcggcctgga ccaccggtac cgggcgattt
     7681 ggaatgcgga tatgagtgag caccgggaca tcgagctcac gcgcgagcgc ttcagcagaa
     7741 gccggtcctt gcgcgagtcc gaccgaaagc agcccggcgc gacggatgac gtccacgacc
     7801 gcccgcacct ggtgagcctc cggtgcaggg cctagcgccg aaagatccag acacaccgcg
     7861 gtgcgttcga agaagtgtgg cgcggtggca gtgcggccgg tgagctcatc gagaatggcg
     7921 cccggatcca ctgtgtgcaa tcgcagctgc acgagcccga cctggccgaa acggatctca
     7981 atggcttgcg ggcgcgcggc gtcggctggt gtattcatcg agggcataca gtctatgttg
     8041 ccttcgagaa tgggtccatc acgaaatggc ccgcagcgcc tgagcgagct tgcgcgccca
     8101 tcgctcctgt gatgcagcgt cgccgacgag atccgcgcgc aactccagca gcacggaacg
     8161 caaaccgcgc accgagcaat gtcttccaag cgtgtgataa accccatcgg tgggcgcgta
     8221 cggctcattg tcgccgacgt tcatgtccgg gtccgcttgc agcaggcgca gcagcggctc
     8281 cgccatcctg cgatcgcgat cggagagcac gccgacgtgc cacggccgat gatgcccgcg
     8341 gtagatgggc gtgaagctgt ggatcgacac cacttgcaga tcggcatgcc gcgcaacctg
     8401 cgtatcgacc gtccgctcga gacagtgatg atagggttcg tagatgtcgc gtatgcgccg
     8461 catacgatcc tctgcagtga tcgcgacatt gccagggatg cgggtatcct cgctgaggga
     8521 cactatggag cctgcgtggt cgggatcgcg attcacatcg agcaccagcc gggagatccc
     8581 ggcatgaacc agcggcgcat ccagcaagcg cgacaggctc tgcgccaccg gcagcgcacc
     8641 cggatcccag gcgatgtgcc ggtcgagctc ctgcggcgcg aggccgaggc gcgccagggc
     8701 gggcggcacc cagttggatg cgtgatcgca gacgaggacc agcgggccgc ggccctgtgc
     8761 gttctcgacc gcaaccggta cgcgcccgag ccaggaagct tgattcggca cggcgcgaat
     8821 cttagaccat gaacctttcg cccggctcgt gaattgatcc tcgcaagaca ctgcgccgct
     8881 ctccggagct cacgatgatt cgtgcccacg ctctctacac tctcctcgcc gcagcggtgc
     8941 tgctgccggg ttgccacgga ggcgatcaag gcaacgctcc aggtacctcg ggtccaccgc
     9001 cgcggggaac gctgctcatc cggcccccgc cgatgctcgc ctcctattcc acggcggacc
     9061 tgctcaagag cctgggcggc aacgatctgg gcaaggagct gctgaccctc gcatacagcc
     9121 cgatctgcac ggtcaacgtc taccagctgg agttcgagac cgtgggcggg cagggcgagg
     9181 cgacgaaatc ctcgggggcg ctgatgatcc cggtcggtct gaattcagcg tgcaatggac
     9241 cgaagcccat cgtgctgtat gcccatggaa ccacggcgga caagaccttc aacatcgccg
     9301 cgctgaacca gtcgaatgac gccgagggat tgttgctcgc agccgtgttc gcagcccagg
     9361 gatacatcgt ggtggcgccc aactatgcag gctacgacac ctcggacctg ccctatcacc
     9421 ccttcctcga cgccgatcaa cagtcgaagg agatgatcga tgtgctgagc gctgcacgcg
     9481 atgcgcttcc gatcgcttcc gctcccggcg tgagcgacaa ccacaaattg ctgctgaccg
     9541 ggtactccca gggcggcttc gtcgccatgg ccgcccagaa ggcaatggag gctgccggag
     9601 tcaccgtgac ggccgctgcg ccgatgtcgg gaccatacgc gcccgctgct ttcgccgacg
     9661 ctgtcttcat gggccaggtc aacctgagtg cgacactcaa tgtgaccctg ctcgtgtcca
     9721 gctatcaaca cgtctacggc aacatctatc agcagccgac tgacctgttc tcggccgcct
     9781 acgccgggga catcgagtcc ctgctgccga gcacgactcc gatcagcgag ttgtattccg
     9841 aaaggaaact gcctgaaagc gagctgttca gcaacgtgcc gccggcgccg gagttcgcct
     9901 cactgacgcc ggcgacgacg ccggccgcac tggctccggc attcgcgctg ggtttcggcc
     9961 ccgatgcgct catcaccaac gactatcgcc tggcgtatct gcgggacgag caggccaacc
    10021 cggatggcgg cttcccgacc gtcagcacgg gggtaccggc agaaaccccg gccaacactt
    10081 tccgacagga cctgaagttg aacgatctgc gcagctggac gcccggcgca cccgtgctgc
    10141 tgtgcgccgg ggatcaggat cctaccgtgt tttacctgaa taccagtttg atgcagagat
    10201 actggatgat gaacgctccg ggagcagtcg tgaccgtcct ggatgttgac ggtccggtaa
    10261 cgagcgggga tccttacgcg acgctgaaga aagcgttcgg cgcggccaaa gcgctgaccg
    10321 cagccgacgc ggtactcgga ggcgcaaaag acggcggcgc gatggcggtt ctgcaggcgt
    10381 atcatgccgg cctcgtgccg ccgttctgcc tgagtgcggt aaaatcgttc ttcgatggat
    10441 tctgaacaga gggtgacatg gcaaccaagc ttgacaaaca actgaagcgc gagattgaca
    10501 tcgatggcaa gccatacatg gttactcttt cgcccgaggg agtgaagttg acggagaaag
    10561 gcaaacgcaa aggtcaggag ctctcctgga agggactcgt gaatggtcag gctgccactg
    10621 cagtctccct cgccggctcg ggacccgagg ccggccaatc gggaagcgaa atgggctgaa
    10681 acccgcccag cttccttcag gcgcctgccg tcgccggcag gcaacccgtc accggtgcgc
    10741 gctgcttgat cgtcttcatg cagacggttg tctcgatccg ctgcacgccc ggcatgcgcg
    10801 agatctcacg ccgtaacagc gcatccagct cggatatccc cgctacgagg acgtgcatca
    10861 ggtagtccgc ctggccagcc gtcgtgaagc attgcaggat ggccggatgt ctctgaaccg
    10921 cgctttcgaa gtcatcatgg cgtgccgagc tgatcgtgac gtggacgaaa gcgtgcacat
    10981 gcagcccgag ttgcgcggcg tcgatctcgg ctcggtatcc cttgatgacc cctgcccgct
    11041 cgagatcccg cacgcgttgc cagcagggtg tcttcgacag gccgatctgc tggcccaggt
    11101 cggcgaagga ctgccgtgct tccgcctcga gcagcgtaat gatccgatgg tcgatcttgt
    11161 ccaagctcgt ccttccagat cgctactgtg gagatcaatg ttctaccgcg acgcgcgctt
    11221 tcagtccagc cggctgagcg ctctggccac cggcggagcg gtcgggacag ggactccggc
    11281 tcagaggctt gccgggagca cggcccaagg ctgacaggca cgcggaaacc tgctttagtg
    11341 agtacggagg caacaaaatc catagcgagc gcctccgggg agagcccatg gccacacctg
    11401 cccagtacgc tcagaaccaa caggcatccg gcatcgatcg cgcgcatcgc agcgtgatct
    11461 ttgcctcttc gctcggaacg gttttcgagt ggtacgactt ctatctgtac ggatcgctgt
    11521 cggcagtgat ctccaagcaa ttcttctccg gagtcaatga gacgagcggc ttcatctttg
    11581 cgttgctcgc cttcgccgcc ggattcttcg tgcgaccctt cggcgctctc gtgtttggcc
    11641 gggtcggcga tctcgttggc cgcaaataca catttctgat caccatcgtg atcatgggta
    11701 catcgaccgc tctggttgga gtgctgccca gctacgcttc gatcggcgtc gccgcgccgg
    11761 cgatcctgat ctcgctgcgg ctgctacagg gcctggcact cggtggcgag tacggcggtg
    11821 cggccaccta tgtggcagag cacgcacctg ccggccgccg gggactgtac acgagctgga
    11881 ttcagaccac ggcaacactg ggactgtttc tgtcgttgct cgtgattctc gtgtgccggg
    11941 tctccctggg tgcgcagttc gagagctggg gctggcgcat cccgttcctc gcctccatcg
    12001 tgctgctggc catttcggtc tacatccgcc tgcagttgcg tgaatcgccg gctttcctcg
    12061 agatcaagag ccagggcaag ctctccaaag cgccgctcac cgaggcgttc ggccagtgga
    12121 gcaatctgaa gatcgtgctg ctggcgctgt tcggcgcgac cgcgggccag gccgtcgtct
    12181 ggtatggcgg ccagttctat gcgctgttct tcctggaaaa gatcctccag gtcgactcca
    12241 aggctgccaa cctgatcatc gccgccgcgt tgctcatcgg tacgccgttc ttcgtcgtgt
    12301 tcggctccct gtcggatcgc atgggtcgca aaacgatcgt catggccggc tgtgtgctgg
    12361 cggtcttcac gtatttcccc gtgttcaagg ggctgacgca ctatgccaat ccggcgctgg
    12421 aggctgccgt ggccacgagt ccggtcaccg tgatcgccga tccgggcgac tgctcgttcc
    12481 agttcgatcc ggtgggaaag aagaccttca cccgctcctg cgatgtcgcg aaggcggcgc
    12541 tcgccaaagc cggcgtcccg tacgcgaatg aggcggcgcc ggccagctcg gtcgcgcgtc
    12601 tgcgcatcgg caccggcgcc caggcagtgt ttgtcgattc ctttgccggt aacaccctag
    12661 cgaagcccga cttcaagact gcgaacgagg ccttctccaa gcaactgaag ggtgcgctcg
    12721 cgcaggcggg gtatccgccg aaagccgaca tgtcgcgcct cgactatccg atggtcatcc
    12781 tgctgtgcgc ctacctggtc gtactggtta cgatggtgta cggaccgatc gccgcgtggc
    12841 tggtggagct gttcccgacg cggatccgct actcgtcgat gtcattgccg taccacatcg
    12901 gcaacggctg gttcggcggg ttcctgcccg tgacctcgtt cgcgatcgtt gcggccaccg
    12961 gtgggatcta caacggtctg tggtacccgg tcatcgtggc ggcagcgact gccatcatcg
    13021 gtactctgtt cctgcgtgag acacgcggga cgagccttgc aacctgatca gatgaggtac
    13081 tgcggtgagt gaagagggaa tgcgcgagct gctcgtgaaa gttcacgagc ggttacgcac
    13141 gtccggctct ctcgatgccg atgcgcgcga actcctgttg accgtgatgc gggacatcga
    13201 ggcggcgctc ggtgctgccg acgccggcgc ctcgcgcacc cgagtctctc acgtacctcg
    13261 gctcgaggcg ttggcggttc gctttgaggc aggacatccc gggctggcgc aggtgttgcg
    13321 gcagctcgca accgtgctgg gccaggccgg gctttagcgt cagtcgtcca tcaggaagct
    13381 gcgcagctgc tcgctgcggc tcggatgccg cagcttgcgc agtgccttcg cctcgatctg
    13441 gcggatgcgc tcgcgtgtga cgtcgaactg cttgccgact tcctcgagcg tgtggtcggt
    13501 gttcatgtcg atgccgaatc gcatgcgcag cactttcgct tcacgcggcg tcagttgcgc
    13561 cagaaccgag tgcgtagttt cgcggagcga ctccatcgtc gcctgatcga tcggcgaggc
    13621 cacggaggtg tcctcgatga agtcgcccag atgcgagtcc tcgtcatcgc cgatgggagt
    13681 ctccatcgag atcggctcct tggcgatctt cagcaccttg cgcaccttgt cctcgggcat
    13741 ctccatgcgc acggccagct cctcgggagt gggttcgcgg cccatctcct gcagcatctg
    13801 ccgcgagatg cggttgagct tgttgatcgt ctcgatcatg tgcacgggga tgcggatcgt
    13861 gcgcgcctga tcggcgatcg agcgcgtgat cgcctggcga atccaccatg tggcgtaggt
    13921 gctgaatttg tagccgcgcc ggtactcgaa cttgtccacc gccttcatca ggccgatgtt
    13981 gccttcctgg atgaggtcga ggaactgcag gccgcggttg gtgtacttct tggcgatcga
    14041 gatcaccagg cgcaggtttg cctcgaccat ttccttcttc gcgcgccgcg ccttggcctc
    14101 gccgatggag acatcgcggt tgacttcctt gatgtcgttg atggtcaggt ggtactgcgt
    14161 ctcgagctgc tcgagcttct tctgccgccg ctcgatctcg tccttgaact tcgccagcgg
    14221 cgctgagtac ttcttgccgc tcttgacgtg gcgcgcgagc cagcgcgagc tggtctcgtt
    14281 cttcgggaag gtggagatga aatccttgcg cggcatcccc gcatcgcgca cggcgatgat
    14341 catgatctcc ttctcgagct ggcggacctc ggcgacgtgc gtgcgcaggt tgaggatgag
    14401 cgcatcgaac atacgcggcg agagcttgag ctccatgaac tcgtccgcga gtttcttgcg
    14461 ggtcttgagc gacttggggt cgcgcgcgcc caccttctcg atcgcactca gcgcgtggat
    14521 gtaacccttg ccgatggagg cgaaccgcgt ggcggcttcc tccggatcgg ggcccgtgtc
    14581 caccgcttcc tcttcgctgt cgctcgcgtc ctcgccttcc tcctcgtcgt cggacttctc
    14641 gtccgcctcg accagctcca tcttggtggg gttctgcggc tgcgcgatca catcgggcgc
    14701 attgggatcg atgaagccga cgatcacatc gaccaggcgg gcctggccgg ctttcaccgg
    14761 ctcgtacgcc ttgagcagaa aatcatacgt agcggggtac agggcgagcg cgctgcgcac
    14821 cgcatccagg ccctcctcga tgcgcttcgc gatgcggatc tcgccctcgc gggtcagcaa
    14881 ctcgacggtg cccatctcgc gcatgtacat gcgcac
//
LOCUS       DASRHN010000055         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_514589, whole genome shotgun sequence.
ACCESSION   DASRHN010000055 DASRHN010000000
VERSION     DASRHN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514589"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFB37_02155"
     CDS             complement(<1..345)
                     /locus_tag="VFB37_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZO21278.1"
                     /translation="MLLIRRMEEKLSTDSAAGVLPGAVHLYIGQEAVAAGVCMQLAPD
                     DWITSTHRGHGHFLGKGGDPKAMMAEIWGKKTGICQGMGGSMHVADLSKGILGANGIV
                     GGGLAISVGAAFG"
     gene            complement(405..1259)
                     /locus_tag="VFB37_02160"
     CDS             complement(405..1259)
                     /locus_tag="VFB37_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZO21279.1"
                     /translation="MSNVELIASYWTLAGGALPHTDKEYSTFEFKERVEAAARAGFKG
                     IGLWHADLEHVQKTYTLPQMKRILDDNGMKHIEIEFLADWFLPPGERRAASDQRRQFL
                     FDACEGLGARHLKVGDFYKSQCPMPRLIDEFANLCRDAARRGIRIVYELMPFSVVESL
                     EATRQLVQGAAQKNGGVIFDLWHIVKLGIPYDEVMRFPGEYFFGLELNDGYLRTPPGM
                     DLVTETTSHRKLCGQGEFDIKGFTGKLSSSAYRGPVGIEVLSQELRSWPLEKTATTAF
                     NTTRAQFS"
     gene            complement(1288..>2386)
                     /locus_tag="VFB37_02165"
     CDS             complement(1288..>2386)
                     /locus_tag="VFB37_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HZO21280.1"
                     /translation="LLELARHKAASAKDAADFAAALREAVADVVRRQVAAGIDIVNDG
                     EYGKSSWANYVLERIKGFEEREGRFDARDWLGRERERFPEVIEAEFGQYVMRPVQACV
                     APIDYHDLSDVKRDTQNLNNALAGSSATEGFLTAVAPASAAFNAIDDYYHDERAYVMA
                     MAEALRKEYLEVYRAGLIVQVDDAVLANMYDHLTQKSPREYRKWAELRIEALRHALRD
                     IPEDRIRYHVCFGSWHLPHTVDAPLEEIVDLILQVPAQAYSLEAANPRHEHEWRVWEK
                     VKLPPGKILIPGVITHHLLMVEHPRVVADRIVRFARIVGRENVIAGSDCGFAQQESLQ
                     RVHPKVMWAKFEALAAGARLATSELWGRAAA"
BASE COUNT          427 a          790 c          722 g          447 t
ORIGIN      
        1 accgaacgcc gcgccgacgg aaatcgccag cccgccaccg acgatgccgt tcgccccgag
       61 aatccccttg ctcaggtcgg cgacatgcat cgagccgccc atgccctggc agatgccggt
      121 tttcttgccc cagatctcgg ccatcatggc cttcggatca ccgcccttgc cgaggaaatg
      181 gccgtggccg cgatgggtgc tggtgatcca gtcgtcgggc gcgagttgca tgcagacgcc
      241 ggcggcgaca gcctcctgcc cgatgtacag gtgcacggct ccggggagca cgccggcggc
      301 ggaatcggtg ctgagcttct cctccatgcg gcggatgagc aacatgcgct catagagcgc
      361 cagggattgc gcgggtgtcg gccccgcggt cgatgtgggt tgagtcatga gaattgcgcc
      421 cgcgtggtgt tgaatgctgt ggtcgccgtt ttctccaggg gccaggagcg cagctcctgc
      481 gagaggacct cgattccgac cggcccccgg taggcggaag aggacaattt gccggtgaaa
      541 cccttgatgt cgaactcgcc ctggccacag agcttgcggt gcgaggtggt ctcggtcacc
      601 aggtccatcc cgggcggcgt ccgcagatag ccgtcgttga gctccaggcc gaagaagtat
      661 tctccgggaa acctcatcac ctcgtcatac gggatgccga gcttgacgat gtgccagaga
      721 tcgaaaatga cgccgccgtt tttctgcgcg gcgccctgca cgagctgtcg ggtcgcctcc
      781 agcgactcca ccacggagaa gggcatcagc tcgtagacga tccggatccc gcgccgcgcg
      841 gcatcccggc atagattcgc gaactcatcg atgaggcgcg gcatcgggca ttgcgacttg
      901 tagaagtcgc cgaccttcag atggcgcgcg ccgagtcctt cacaggcgtc aaagagaaac
      961 tgacgccgct ggtcggaggc cgcacgccgc tcgcccggcg gcagaaacca gtccgccagg
     1021 aactcgatct cgatgtgctt catgccgttg tcgtccagga tccgcttcat ctgcggcagc
     1081 gtgtacgtct tctgcacgtg ctcgagatca gcgtgccaga gcccgattcc cttgaagccg
     1141 gcgcgcgcgg ccgcttcaac ccgctccttg aattcaaagg tgctgtattc cttgtcagtg
     1201 tgcggcagcg cgcccccggc caatgtccaa taggatgcga tcagctcgac attgctcatt
     1261 ccgctcaccc ccaacgcccg cggtggatca ggcagcagcc cggccccaga gctcgctcgt
     1321 cgcgagacgg gcgcccgcgg cgagggcttc gaacttagcc cacatcactt tcggatgcac
     1381 gcgctgcaga ctctcctgct gcgcgaaccc gcagtcggag cccgcgatca cgttctcacg
     1441 cccgacgatc cgggcaaaac gcacgatccg gtccgccacc acccgaggat gctccaccat
     1501 caagagatga tgggtgatca cgcctggaat aagtattttt ccaggcggca atttgacctt
     1561 ctcccagacg cgccactcat gctcatggcg gggattggcc gcctcgagtg aataggcctg
     1621 cgccggcacc tgcagaatca gatcgacgat ctcctccaga ggcgcatcga ccgtgtgcgg
     1681 cagatgccaa ctcccgaaac aaacgtggta gcggatgcgg tcctcgggaa tatcgcgcag
     1741 agcatgccgg agcgcctcga tgcgcagctc agcccacttg cgatattctc gtgggctctt
     1801 ctgcgttaga tgatcataca tgttggcaag gacagcatcg tccacctgca cgatcagccc
     1861 tgcgcggtag acctccagat actctttccg caaagcctcg gccatggcca tgacgtaggc
     1921 gcgctcgtca tggtagtagt cgtcgatggc gttgaatgcc gcgctcgccg gcgccaccgc
     1981 ggttagaaag ccttccgtcg cactacttcc cgcgagcgcg ttgttcaggt tctgcgtgtc
     2041 ccgtttgacg tcgctgaggt cgtgatagtc gatcggggca acacacgcct gcactggacg
     2101 cattacgtac tgcccgaact ccgcttctat tacctcggga aagcgctcac gctcgcgacc
     2161 gagccagtcg cgtgcatcaa accggccctc ccgttcctcg aagcccttga tgcgctcgag
     2221 cacgtagttc gcccaactcg acttgccgta ttcgccgtcg ttgacaatgt cgatacccgc
     2281 cgcgacttgt cggcgtacga cgtccgcaac ggcttcacga agggctgccg cgaagtcggc
     2341 ggcatctttc gcggacgcgg ccttgtggcg agcgagctcg aggagc
//
LOCUS       DASRHN010000056         9412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_845647, whole genome shotgun sequence.
ACCESSION   DASRHN010000056 DASRHN010000000
VERSION     DASRHN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845647"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VFB37_02170"
     CDS             complement(<1..1365)
                     /locus_tag="VFB37_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21281.1"
                     /translation="MKRLLLLMMSAGLVAVTLAGASLTYPPAARGNHVDTYHGVKVPD
                     PYRWMENIDSPQTRSWVQAEDRLSRAYLDALPGRDAITQRLKRIWNFERWSAPERHGR
                     YWFYTHNDGLQNQAVIFVTTDPNTAGRVLLDPNTLSPDGTVALRETAVSDDGRLFAYA
                     LSDAGSDWQIWHVRNVDSGTNLPDELHWSKAGGGSWRKDGSGFYYTAYDQPRGTEALK
                     AANKYQKMYFHKLGTPQSSDPVVYTRADDPDWFVGGQVTDDGHYLMIEAHHGDEVQNT
                     LLLQDLSIDNAPIKPMIPEPTAVYSFIGNIGSTLYVLTDDGAPRYKVVAIEESKPAPA
                     DWRTVIPEGADTLNEVSLVGGQLLAQYLQDAHSAVRRYTPQGKLLGEVKLAGLGKAAG
                     FQGHEQDHETYYLYSGLTLPPSVFRLDLGSGTSSLWKMPHLQGFSPEEFETRQVFYKS
                     KDGTR"
     gene            complement(1436..1921)
                     /locus_tag="VFB37_02175"
     CDS             complement(1436..1921)
                     /locus_tag="VFB37_02175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO21282.1"
                     /translation="MPPYTIRACTSSDAPTIARHRVEMFRDMGEVPTERAAAAFLAAS
                     IPAIRTELDRGSYYGWFAVSGGEQVIAGVGAHVKATLPRMGADGLHVFSTAVPLVVNV
                     YTEPNWRKRGLATALMRTLMQWAREQSFDRVVLHASDHARSLYASLGFVPSNEMRWRL
                     A"
     gene            complement(1926..2951)
                     /locus_tag="VFB37_02180"
     CDS             complement(1926..2951)
                     /locus_tag="VFB37_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZO21283.1"
                     /translation="MRNASLPLLCTAALAWAAAAQADSPPHYHLTHEVPLPGDDGWDY
                     LAYDSAGGRIFVTHGTKVQAVDSGKLILTGEIGDTPGVHGVAFAHDLGRGYISAGRAN
                     TVVVFDLKTLTVIARIPTGENPDAILYEPTTHRVFTFNGRSRNISAIDTAKNEVIGTI
                     PVDAKPEFAATDGAGHVFVNLEDKSSIASIDPIKLTVTAVWPLSGCEEPSGLAIDVKH
                     RRLFSVCDKAMAVVDSGTGRVVANVPIGSGIDAAAYDPGTRLAFASGGDGTLTVVQEE
                     TPDKFAVVQTVQTRAGARTMALDDRTHRVFLVTAKFGPRPAPTAEQPHPRPSIVPRSF
                     ELLVLDP"
     gene            3020..4282
                     /locus_tag="VFB37_02185"
     CDS             3020..4282
                     /locus_tag="VFB37_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21284.1"
                     /translation="MKKKLHYAWIVLGVTFLCLLVSAATRATPSILIVPLEREFGWSR
                     TLISMAISVNILLYGLIGPFTAGFIERYGPRRVLAVSAVLIGVGTLATITIKHPWQLF
                     ALWGVLVGVGTGSVAIVLGASIVQRWFHAHRGLALGLLTASAATGQLLFLPPLARLVV
                     DHGWRAAVITMAVLALCLAPVALLLVRDRPQDKGLLPLGQLAGARERPPPAQNPFLAA
                     TAALRMASHRREFWILAVSFFICGASTNGLVGTHLVPACMDHGIPEVQAASLLAIMGI
                     FDLLGTTGSGWLSDRFDNRRLLFAYYALRGASLLYLPYGFVSEGHGLSIFAVFYGLDW
                     IATVPPTVALAREAFGPEKAALVFGWIMAAHQIGAALAASLAGLIRTRSGSYDQAFWI
                     AGGLCLLTALGVLFAGRPRKAGLVPQTG"
     gene            complement(4356..4694)
                     /locus_tag="VFB37_02190"
     CDS             complement(4356..4694)
                     /locus_tag="VFB37_02190"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO21285.1"
                     /translation="MIERQSRLTDAVPVFAALGDATRLKIVNRLCGSGPMSIIELTEG
                     SQVTRQAVSKHLRALERAGLVSSGRVGRERIWELRTAPLAEVRAELEAISDRWDQAIG
                     RLRALVQDNP"
     gene            complement(4697..5188)
                     /locus_tag="VFB37_02195"
     CDS             complement(4697..5188)
                     /locus_tag="VFB37_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO21286.1"
                     /translation="MNTDRIEKKVLLQATRERIWRAISDSAEFGTWFGVAFDQPFTEG
                     ARLSGRIVPTAVDAEVAAHQRPYEGRRFEFQVERIEPMTRIAFRWHPFAVEPEVDYSH
                     EPTTLIVFELSEAVGGILLTIVESGFDRIPLARRAKAFSANDGGWEQQTQLIRKYLAR
                     EAR"
     gene            complement(5649..7202)
                     /locus_tag="VFB37_02200"
     CDS             complement(5649..7202)
                     /locus_tag="VFB37_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21287.1"
                     /translation="MNKKTLCLCLLAGCLLGPPLTFAKDKDASSPAGEAGKAAAKDAD
                     KSKEEDIKLPSFPADKSVHQSMTLGGRVIKYDATVGSLPVLDEKGKTIASVMFTAYTV
                     PGANRAVTFAFNGGPGASSVYLNLGAIGPKRVQFGAEGDSPSDPATPQDNAGTWLDFT
                     DLVFIDPVGTGFSYAQVPEAEAKKHFYNARADVGYLSRIVYDWLVKNERLSSRKYLVG
                     ESYGGFRGPRITHYLQTRLGIAMNGMVLVSPYLDPGASSNGDLSPLPWMLTLPSIAAA
                     NLERQGKLNDESMAKIIDYTRTDYVTDLLKGRSDTQAIERIVARVSELSGLDPTFVRH
                     LGGRLETQAYLREVYRSEGKLGSRYDSNVTSWDPFPFAPNQRNNDPILDSIIAPTTTA
                     MVDFITRTVGWKYSGRYNALSYEVNKAWSAGGEGQDGENEIERGSVEQLRESVAIDPK
                     LHVLIVHGWDDLSCPFMASVLIVDQMPLMGDPKRVRVREYPGGHMFYSRSASQAALRG
                     DVMTMYGLH"
     gene            complement(7228..8658)
                     /gene="nuoN"
                     /locus_tag="VFB37_02205"
     CDS             complement(7228..8658)
                     /gene="nuoN"
                     /locus_tag="VFB37_02205"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZO21288.1"
                     /translation="MISSSLAPAIPEMYLVAVICVILLVDAFAGENHRRLTGTLTMLA
                     LAVGAALTVLVAPVTQRTVLFDGMYVADQLGLVLKLAGFLFVAVGLLYSRDYLEERGI
                     LRGECYVLALTALLGIFVLISAHNLITLYIGVELLALSVYSLVAFDRDSGVAAESAMK
                     YFVLGAIASGALLYGMSMIYGMTGTLDLDELALRVHEPASLGAIIGLVFIVVAVAFKL
                     GAVPFHMWLPDVYEGAPTSVTLFISTAPKLAYFALTLRLLAQGLGGTVGEWSQMLGAL
                     AVLTLIVGNVVAIVQTNLKRMLAYSTISNVGFIVLGFVAGTPDGYTAALYYTLIYVLV
                     ALGSFGVILMASRKGFEADQLDHYRGLYRRDPLLAFVMMILMFSTAGVPPFVGFWAKL
                     RIFQALWETNHFWLVVISAAMSVVGAFYYLRVIKLMYFDPPPAHFAPARHSAGIRMTL
                     GLNAAAVLVLGLLPGPLLDLCGRLIH"
     gene            complement(8689..>9412)
                     /locus_tag="VFB37_02210"
     CDS             complement(8689..>9412)
                     /locus_tag="VFB37_02210"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZO21289.1"
                     /translation="APDASRELDLLLISLSLIAVVYIGFVALVQQDMKKLIAYSSIAH
                     MGFVTLGAFVVYEIVRATGAVQGAGMGMDGAMVQMISHGLISGALFLCVGVLYDRMHS
                     RQIADYGGVINTMPIFAAFMVLFALANTGLPGTSGFVGEFLVILASFKASFWYSLLAA
                     VTLILGAAYTLWLVKRVVFGPVANPRVAALTDLNGREFIVLGALALAVLWIGVWPAPL
                     LKIMQPTIHHLVDQAIASKIPV"
BASE COUNT         1707 a         3026 c         2950 g         1729 t
ORIGIN      
        1 ccgcgtgccg tccttgctct tgtagaacac ctggcgggtc tcgaactcct ctggggaaaa
       61 gccctgcaga tgcggcatct tccagagtga gctcgtcccg ctgcccagat cgagccggaa
      121 cacactcggg ggcaaagtca aaccgctata cagataatag gtctcgtgat cctgctcatg
      181 tccctggaat cccgccgctt tgccgagacc tgcgagcttg acctccccga ggagcttgcc
      241 ttgtggcgtg tagcgacgca cggcgctgtg ggcatcctgc aggtactgag cgaggagctg
      301 gcctccgacg agactgacct cgttcagtgt gtcggctcct tcaggaatca ccgtgcgcca
      361 gtcagccggt gccggcttcg attcttcgat cgccacgacc ttgtaacgcg gcgccccatc
      421 atcggtcagc acataaagtg tcgagccgat gttgccgatg aagctgtaca cggccgtcgg
      481 ctcgggaatc atcggtttga tcggcgcgtt gtcgatcgac aggtcctgca gtaaaagtgt
      541 gttctgcacc tcatcgccgt ggtgggcctc gatcatcagg tagtgtccgt catcggtgac
      601 ctgaccgccg acgaaccagt cggggtcgtc cgctcgcgtg tacaccaccg ggtcactcga
      661 ctgaggcgtc cccagcttgt ggaaatacat cttctgatac ttgttcgcag ccttgagcgc
      721 ctcggttccg cggggctgat cgtaagcggt gtagtagaag cccgagccgt ccttgcgcca
      781 gctgccgccg ccagctttcg accaatgcag ctcgtccggc agatttgtgc ccgagtcgac
      841 attgcgcaca tgccagatct gccagtccga gcccgcatcc gacaaggcat aggcgaacag
      901 ccgaccgtcg tcgctcacgg ccgtttctct cagcgcgacg gtgccatccg gcgagagcgt
      961 gttcggatcg aggagcacgc gccctgcagt gttgggatcg gtggtcacaa agatcaccgc
     1021 ctgattctgc agtccgtcat tgtgcgtata gaaccagtat cggccgtgcc tttcgggcgc
     1081 gctccagcgc tcgaagttcc agatccgctt caggcgctgg gtgatcgcgt cacggcccgg
     1141 caacgcatcc agataggcgc gactgagccg gtcctcggcc tgcacccagg aacgcgtctg
     1201 gggcgaatcg atgttctcca tccagcggta cgggtcgggc accttgaccc cgtgataagt
     1261 gtcgacgtga ttgccgcgcg ccgcaggcgg ataggtgagc gaggccccgg cgagcgtcac
     1321 cgccacgagg cctgccgaca tcatcaacag cagcagtctc ttcacgctcg agctccattc
     1381 tggtttgttg gaaatcgccc gcaacgattt tcgtgcacgg accctggcgc cctcttcatg
     1441 cgagccgcca gcgcatctca ttgctgggaa cgaaacccaa cgatgcatac agcgagcgtg
     1501 cgtggtccga tgcgtgcagg accacgcggt cgaagctctg ctcccgcgcc cattgcatga
     1561 gggtacgcat cagggcagtg gccagaccgc gctttctcca gttcggctcg gtatagacat
     1621 tgactacgag cggtacggcg gtgctgaaga cgtgaaggcc atccgcgccc attcgcggca
     1681 acgtcgcttt gacgtgcgcg cccacgccgg caatgacctg ctcgccgcca ctgaccgcga
     1741 accagccgta atacgacccg cgatccagct ccgtccggat ggcgggaatc gatgccgcga
     1801 gaaaggccgc ggcggcccgc tcggtcggga cctcccccat gtctcggaac atctcgacgc
     1861 gatgtcgtgc gatcgtcggc gcatcgctcg aagtgcaggc tcgaatcgtg tacggaggca
     1921 tcccgtcagg gatccagcac cagcagctcg aagctgcgcg gcacgatgct cggacgcgga
     1981 tgcggctgct cggcggtggg tgccggccgt ggcccgaact tggcggtcac cagaaacacc
     2041 cggtgcgtgc ggtcatcgag cgccatcgtg cgcgcgcccg cccgagtttg aaccgtctgc
     2101 accacggcaa acttgtccgg agtttcctct tggacgaccg tgagcgtccc gtcgccgccg
     2161 gaagcgaagg ccagccgtgt acccggatcg taggctgcag cgtcgatacc gctgccgatc
     2221 ggcacattcg cgaccacccg gccggtgccc gagtcgacca ctgccatcgc cttgtcgcac
     2281 acgctaaaca accgacggtg cttgacgtcg atggcaagcc cgctcggttc ttcgcaaccg
     2341 ctgagtggcc acacggcggt gaccgtgagt ttgatcgggt cgatggaggc gatgctgctc
     2401 ttgtcctcca ggttcacgaa cacatgtccg gcgccgtcgg tggctgcgaa ctccggcttc
     2461 gcatcgacag gaatggttcc gatcacctcg ttcttcgccg tgtcgatcgc ggaaatattg
     2521 cggctgcggc cgttgaaagt gaacactcga tgtgtcgtcg gctcgtacag aatggcatcc
     2581 gggttctctc ccgttgggat gcgggcgatg accgtcagcg tcttgagatc gaagaccacc
     2641 accgtgttgg cccggccggc gctgatgtag ccgcggccca gatcgtgcgc gaacgccaca
     2701 ccgtgcactc ccggcgtatc gcctatttcg ccggtcagaa tcagcttgcc gctgtccact
     2761 gcctgcacct tcgtgccatg agtcacgaag atgcgcccgc ccgcactgtc gtaagccaga
     2821 tagtcccagc cgtcatcgcc gggcagcggg acctcatgag tcaggtggta atggggtggc
     2881 gagtctgcct gcgcggccgc agcccaggcc agcgcggccg tgcagagaag tggcagtgaa
     2941 gcgtttctca tcgtcagctc cggagatctc gatctttggg gatacagaac attataaggc
     3001 ctgtctgtac acttcctgtg tgaagaagaa gctgcactac gcctggatcg tccttggagt
     3061 gactttcctc tgcctgctgg tctcggcagc tacgcgcgct acgcccagca tcctgatcgt
     3121 gccgctcgag agggagttcg gttggtcgcg cacgctcatc tcgatggcca tctcggtgaa
     3181 catcctgctc tacggcctga tcgggccgtt cacggccggc ttcatcgagc gttacggacc
     3241 ccgacgcgtg ctggcggtat cggcagttct catcggggtc ggcacactcg ccacgatcac
     3301 catcaaacat ccctggcagc tgttcgcgct gtggggcgta ctggtcggtg ttggcacggg
     3361 tagcgtagcc atcgtgttgg gagcgagcat cgtgcagcgc tggtttcatg cgcatcgtgg
     3421 cctcgctttg ggtttgctga ccgcgagcgc agcgaccggg cagctgctgt tcctgccgcc
     3481 gctcgcccgg ctcgtggtcg atcatggctg gcgggcggcc gtgatcacga tggcggtgct
     3541 ggcattgtgc ctggctccag tcgctctgct cctggtgcgc gatcgcccgc aggacaaggg
     3601 gctgctgccg ctcggacagc tggcgggcgc gcgggagcga ccaccgcccg ctcagaatcc
     3661 gttcctcgcg gccaccgccg ccttgcgtat ggccagccac cggcgggaat tctggatact
     3721 cgccgtcagc ttcttcatct gcggggcgag caccaacgga ctcgtgggca cccatctggt
     3781 gcccgcgtgc atggatcatg gaatcccgga ggtgcaggca gcttccctgc tcgccatcat
     3841 gggcatcttc gacttgctcg gaacgacggg ctcgggctgg ctctccgacc gcttcgacaa
     3901 tcggcggctc ctgtttgcgt actacgcgct caggggtgcc tcgctgctgt atctgcctta
     3961 cggcttcgtt tccgaaggtc atgggctgtc aatattcgcg gtcttctacg gactggactg
     4021 gattgcaacc gtgccgccaa cggttgcgct cgcccgtgaa gcgttcggcc ccgaaaaggc
     4081 ggctctcgtc tttggctgga tcatggctgc tcaccagatc ggagccgcac tcgcagcctc
     4141 cctcgccgga ctgatccgca cacgcagcgg cagctacgat caggcgttct ggatcgccgg
     4201 cgggctgtgt ctcctgaccg ccttgggagt gctgttcgcg ggccggccgc ggaaagcggg
     4261 actcgtgcct caaacaggat aggcggccgg gctggactat tgaatgtctc gcgagggggc
     4321 tagcagcgtg agcccgcggg cgtgcgtggc tgcgactagg gattgtcctg caccagcgcg
     4381 cgcaagcgcc cgatcgcctg atcccatcga tccgagatcg cctcgagctc agcccggacc
     4441 tccgccagag gggccgtacg aagctcccag atgcgctcgc gacctacgcg gccgctgctc
     4501 acgagccctg ctctctcgag cgcgcgcaga tgcttgctca ccgcctggcg ggtgacctgt
     4561 gagccctcgg tcagctcgat gatcgacatc gggccgctgc cgcacagccg gttgacgatc
     4621 ttcagccgag tcgcatcgcc cagcgcggcg aataccggaa ccgcatcggt gagcctcgac
     4681 tgtcgctcta tcacggctag cgcgcctctc gggccaggta cttgcggatg agctgagtct
     4741 gttgctccca gcccccatcg ttggcgctaa acgccttggc gcggcgggcg agaggaattc
     4801 gatcgaaacc ggactcgacg atcgtcagca ggattccccc cacggcctcg ctgagttcga
     4861 acacgatgag cgtcgtgggt tcgtgagagt aatccacctc aggctcgact gcgaaaggat
     4921 gccaccggaa ggcaatccgg gtcatcggct cgattcgctc gacctggaac tcgaatctcc
     4981 tgccttcata gggccgctgg tgcgcggcca cctcagcgtc caccgctgtg ggcacgatgc
     5041 gtccgctcag ccgcgcgcct tccgtgaagg gttggtcgaa agccacacca aaccacgttc
     5101 caaactccgc ggaatcgctg atggcccgcc agatgcgctc gcgggtggcc tgcagcagca
     5161 ccttcttctc gattcggtcg gtattcatat acaacctcca ggttgctcgt tataccggct
     5221 ggcggggaat tatgcaaccg gttggttgcc tgtcttcagg ctctgcgtgg ggagcgactc
     5281 cgcgctgcgg ttgctgtggg acgaactcgt tgccgagcgg gtctgatccg tcgggtagag
     5341 ctttcgctca tgcggtgcag agcgtctgat tgtggggctg aagacacctc ggggagttgc
     5401 aggaatgaag catctcggtg tggtctgggc cctcgtggca ctcgttgcga cgagtctggc
     5461 cttgagtgag gtgcaacagc cagggccgat cgcgcacgag attacggcag cggtgcaggg
     5521 cccatcgcgc tccccatccg ataccgaacg cgatgccgac gggaagcccg ctgaggtgtt
     5581 ggctttcgct ggcctcagag caggtcacaa ggtggcagag tatgccgccg gtaccgggtc
     5641 tcgcctgctc aatgcaggcc atacattgtc atcacatccc cacgcaaggc cgcctgacta
     5701 gccgaacggc tatagaacat gtgaccgccg gggtactccc gtacccgcac gcgtttggga
     5761 tcccccatga gcggcatctg atccacgatc agcaccgagg ccatgaaagg gcacgacagg
     5821 tcgtcccagc catgcacgat cagcacgtgc agcttggggt cgatcgccac tgactcgcgc
     5881 agctgctcga ccgagccccg ctcgatttcg ttctcgccgt cctggccctc gccgccggcc
     5941 gaccaggcct tgttcacctc gtagctcaga gcgttgtaac gcccactgta cttccagccg
     6001 accgtgcgcg tgatgaagtc aaccatcgcc gtggtcgtcg gagcgatgat gctgtcgaga
     6061 atggggtcgt tgtttcgttg gttcggggcg aacggaaatg gatcccagct cgtcacgttg
     6121 gagtcgtagc ggctgcccag tttgccttcg gatcgataga cctcccgcag gtaagcctgc
     6181 gtctcgaggc gaccccccag gtgccgcacg aaggttggat cgaggccgga cagctccgat
     6241 acccgcgcga cgatgcgctc gatggcctgc gtgtcggagc gccccttgag cagatccgtg
     6301 acgtaatcgg tccgggtgta gtcgatgatc ttcgccatgg actcatcgtt gagcttgccc
     6361 tggcgctcga gattggcggc cgcgatgctc ggcagcgtca acatccacgg cagcggtgac
     6421 agatcgccgt tgctggaagc cccgggatcg agatagggcg acaccagcac catgccattc
     6481 atcgcgatgc cgaggcgggt ctgcaggtaa tgcgtgatgc gcgggccacg aaagccaccg
     6541 tagctttccc cgaccagata cttgcgcgag ctcaggcgct cgttcttgac cagccagtca
     6601 tagacgatgc gcgatagata cccgacatcg gcgcgcgcgt tgtagaaatg cttcttcgcc
     6661 tccgcttccg gaacctgcgc atagctgaaa cccgtgccga cgggatcgat gaagaccagg
     6721 tcggtgaagt cgagccaggt tccggcattg tcctggggcg tggccgggtc ggaaggactg
     6781 tcaccctccg caccgaactg cacgcgcttg ggaccgatcg ccccgagatt gagatacacc
     6841 gatgaggccc ccgggccgcc gttgaaagca aatgtaactg cacggttagc gcccggaacg
     6901 gtgtaggcgg tgaacatgac actggcgatc gtcttaccct tctcatccag caccggcagc
     6961 gagcccaccg tggcatcgta tttgatgacc cggccgccga gcgtcatgct ctgatgaacg
     7021 ctcttatcgg cgggaaatga aggcagcttg atgtcctcct ctttcgactt gtcggcatcc
     7081 ttcgcggccg ccttcccggc ctcacctgcc gggctggagg catccttgtc cttcgcgaac
     7141 gtcagaggcg gccccagcag gcagccggcg agcaggcaca ggcagagcgt cttcttgttc
     7201 atggttgggt ccgaacgtcc ctgaaggtca gtggatcagt cgcccgcaca ggtccagcaa
     7261 gggtccgggc agcaggccca gcacgaggac ggccgcagcg ttcaggccga gggtcatgcg
     7321 gatgccggcg gagtgccggg ccggggcgaa atgtgccggc ggaggatcga agtacatcag
     7381 cttgatcacg cgcaggtagt agaacgcgcc cactaccgac atcgctgccg agatcacgac
     7441 cagccagaag tgattcgttt cccacagcgc ctggaagatg cgcagcttgg cccagaaacc
     7501 gacgaaaggc ggcaccccgg ccgttgagaa catcaggatc atcatcacga atgcgagcag
     7561 cggatcgcgc cggtagagtc cccggtaatg atcgagctga tccgcctcga accctttgcg
     7621 gctcgccatc aggatcacgc cgaaggaccc gagcgcgacc agcacataga tcagcgtgta
     7681 gtaaagcgcc gccgtgtagc cgtcgggcgt gccggccacg aagccgagca cgatgaagcc
     7741 gacgttggag atggtcgagt aggccagcat ccgtttgaga ttggtctgca caatcgcgac
     7801 gacattgccc acgatcaggg tgagcacggc aagggcgccc agcatctggg accattcccc
     7861 gacggtcccg cccagtccct gagcgagcag gcgcagagtg agagcgaaat aggcgagctt
     7921 gggtgccgtg ctgatgaaga gagtaacgct cgtaggcgcg ccctcgtaca catccggcag
     7981 ccacatgtgg aacgggactg cgccgagctt gaatgccacc gccacgacga tgaacaccag
     8041 gccgatgatt gctccgagac ttgccggctc gtgcacccgc agggcgagct cgtccagatc
     8101 caacgtgccg gtcatcccgt aaatcatgga cattccgtac agcagcgccc ccgatgcgat
     8161 cgcgccgagc acgaagtact tcatggcgga ttcggcagcg acgcccgagt cccgatcgaa
     8221 ggccaccagg gagtagacgg acagcgcaag aagctcgacg ccgatataga gcgtgatgag
     8281 attgtgagcg gagatcagca cgaagatacc gaggagcgcc gtcaaggcga gcacgtagca
     8341 ctcgccgcga aggatgcccc gctcctcgag gtagtcgcgt gaatagagaa gtccgacggc
     8401 gacgaacagg aatcccgcca gcttcagtac caggccgagc tggtctgcca cgtacatgcc
     8461 atcgaacagc acggtgcgtt gggtgaccgg tgcgaccagc acggttagtg ccgcccccac
     8521 cgcgagcgca agcatcgtca gcgtaccggt caggcgccga tggttctcgc cggcaaaggc
     8581 atccaccagc agaatcacgc agatcactgc aaccagatac atctccggaa tggccggcgc
     8641 caggctcgag gaaatcatcg tcgtgtcctg cataagctca tcggtgcctc acaccggtat
     8701 cttgctggcg atggcctggt ccaccagatg gtggatcgtc ggctgcatga tcttcagcag
     8761 tggcgcgggc cagacgccga tccacagcac tgccagcgcg agcgcgccga ggacgatgaa
     8821 ctcgcgtccg ttcagatctg tcagcgcagc cacgcggggg ttcgccaccg gtccgaagac
     8881 cacccgcttc accagccaca aggtgtaggc cgcaccaagg atcagcgtga ccgcggcgag
     8941 cagcgagtac cagaagctgg ccttgaagct ggccaggatc accaggaact cgccgacgaa
     9001 tcccgaagtg ccgggcagcc ccgtgttggc cagcgcgaac agcaccatga acgcggcgaa
     9061 gatcggcatc gtattgatca cgccgccgta gtcggctatc tgccggctgt gcatgcggtc
     9121 gtaaagcacg ccgacacaca ggaagagcgc cccggagatc agtccgtgcg agatcatctg
     9181 caccatcgcg ccatccatgc ccatgcctgc accctgcacg gcgcccgtgg cacgcacgat
     9241 ctcgtaaacg acgaatgcgc cgagcgtcac gaaccccatg tgcgcgatcg aggagtaagc
     9301 aatcagcttc ttcatgtcct gctgtacgag cgcgacgaag ccgatgtaca ccaccgcgat
     9361 cagtgacagc gagatcagca gcagatccag ctcccggctg gcatccggcg ca
//
LOCUS       DASRHN010000057         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_186871, whole genome shotgun sequence.
ACCESSION   DASRHN010000057 DASRHN010000000
VERSION     DASRHN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186871"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1516)
                     /locus_tag="VFB37_02215"
     CDS             complement(149..1516)
                     /locus_tag="VFB37_02215"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO21290.1"
                     /translation="MSRTRARQLVASVALVACAAFAWLWLRPASPARIAPQQVIHDEA
                     PNPNPQPALAQTAAPPGQVPTLQPVSSPVHPSGTTAAALRAGAASPAAATLDVIVNRN
                     DTLDRIFRKLNLSVADLAFLRALPGIKTALDRLRPGESLHFTYRDTELDGLERRLNEN
                     ETLKVTREDGTLQASVLQNPLEGRTRTIRGVIKSSLFEAVTAAGAHDQTAVNLADIFG
                     WDIDFVLDIQPGDSFAVTYRELYQNGAYVKDGPVLAAKFVNQGRTYLAVRYVDPEGNA
                     HYFNPEGKSMRKAFLRAPLEFSRVSSRFNSARLHPILNLIRAHKGVDYAAPIGTPVRA
                     AGDGRISFAGPKGGYGNVVEIEHSRSVTTVYGHLSRFASGTHVGRHVTQGTVIAYVGM
                     TGLATGPHLHYEYRVNGVFKNPQTVALPDAEPIEARWREDFLSKSEPLLATVETPLGT
                     ALVAR"
     gene            1644..2852
                     /gene="tyrS"
                     /locus_tag="VFB37_02220"
     CDS             1644..2852
                     /gene="tyrS"
                     /locus_tag="VFB37_02220"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZO21291.1"
                     /translation="MTDIDSQLAELERGTQEILVTEELVRKLRRQKPLHIKAGFDPTA
                     PDLHLGHTVLLNKMRQFQQLGHTVTFLIGDFTGLIGDPSGRNATRPALTPEAVQANAR
                     TYEQQVFKILDPQRTRVDFNSRWLSSMTAPEFVRIAAQYTVARMLERDDFAKRYRAGQ
                     AISIHEFLYPLAQGYDSVALRADVELGGTDQKFNLLVGRHLQEAYGQEPQVVITMPLL
                     EGTDGINKMSKSQNNYIGIAEDPTVMFGKIMSISDELMWRYFELLSFRPLGELAALRR
                     ATQEGRNPRDVKFELASEIVSRFHDAQAAERAQKEFIARVSLKEVPDDLEPLTVFVDG
                     AGLRLANVLKAAGLAASTSEANRKIDEGAVRIDGTRTTDRSLTLAAGADHVLQVGSRR
                     FARLRLELKP"
     gene            3275..>3456
                     /locus_tag="VFB37_02225"
     rRNA            3275..>3456
                     /locus_tag="VFB37_02225"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          627 a         1051 c         1152 g          626 t
ORIGIN      
        1 aggaacgaca gctcgcgatt gatgtagtgc tggggtgcct tgagatcggg cgagtccatc
       61 gatggattac ctcgtcgcgc gcttcagctt acggccagct gctcgagggc gcggctgagc
      121 acggctccga tgacagcttg atgacacctc agcgggccac cagcgccgtg ccgagcggag
      181 tctccacggt cgccagcagc ggctcggact tgctcaggaa gtcctcgcgc cagcgggcct
      241 cgatcggctc cgcatcgggc aatgccaccg tctggggatt cttgaatacg ccgttcaccc
      301 gatattcgta gtgcagatga ggtccggtcg caagtccggt catgccgacg taggcgatca
      361 cggtgccctg ggtcacgtgc cggcccacgt gcgtcccgct cgcaaagcgc gacaagtggc
      421 catagaccgt cgtgacgctg cgcgaatgct cgatctccac gacgttgccg tagcctcctt
      481 tgggcccggc gaagctgatt cggccatccc ccgccgctcg caccggcgtg ccgatcggcg
      541 ccgcatagtc cactcccttg tgtgcacgga tcaggttgag aatgggatgc agacgcgcgc
      601 tgttgaaacg cgagctgacg cggctgaact cgagcggcgc acgcaggaac gccttgcgca
      661 tgctcttgcc ttccgggttg aagtaatggg cgttgccttc cggatcgaca tagcgcacgg
      721 cgagataggt gcggccctgg ttgacgaact tcgccgcgag caccggcccg tccttcacgt
      781 aagccccatt ctgatagagc tcgcggtagg tcacggcaaa cgagtccccc ggctggatgt
      841 ccagcacgaa gtcgatgtcc cagccgaaga tgtcggccag gttgacggca gtctgatcgt
      901 gggcaccggc agccgtgacg gcttcgaaga gcgagctctt gatcacgccg cggatggtcc
      961 gggtacggcc ctcgagggga ttctgcagca cgctggcctg cagagtgccg tcctcccgcg
     1021 tgaccttgag tgtctcgttc tcattcagcc ggcgctcgag cccgtcgagc tccgtgtccc
     1081 gataggtgaa gtgcagggac tcgccgggcc gcaagcggtc cagggccgtc ttgatcccgg
     1141 gcagcgcgcg caggaaggcg agatccgcga cactcaggtt cagcttgcgg aagatgcggt
     1201 ccagggtgtc gttgcgattg acgatcacgt cgagcgtggc ggccgcgggc gaggcggccc
     1261 cggcccgcag agcggccgcg gtggtaccgc tggggtgcac agggctgctc accggctgca
     1321 gggtggggac ttggcctgga ggcgcagcgg tttgcgccag ggcgggttgg gggttcgggt
     1381 ttggcgcttc gtcgtgaatg acttgctgcg gcgcgatgcg ggcgggcgat gccggtcgca
     1441 gccagagcca ggcgaatgcc gcgcacgcca cgagcgccac actggccacg agctgccgcg
     1501 cgcgcgtccg ggacatcccc gcttccgagc gagccaattt gaacaggtcc ggtttgaagc
     1561 ggagggccat cgactacact tggcgtgccg cgggtgcgga aaccgtaccc gagcggcaaa
     1621 cgttcggtca attttgggca gacatgacgg atatcgacag ccaactggcc gagcttgagc
     1681 gcggtacgca ggaaatcctg gtcacagaag agctcgtgcg caagctgcgc cgacaaaagc
     1741 cactccacat caaggcaggc ttcgacccca cggcgcccga tctgcatcta gggcatacgg
     1801 tcctgctgaa taagatgcgg cagttccagc agctcggcca cacggtcacc tttctcatcg
     1861 gggacttcac cgggctcatc ggtgatccga gcggccgcaa cgccacccgc ccggccctga
     1921 ccccggaggc ggtgcaggcc aatgcgcgga cgtacgagca gcaggttttc aagatcctgg
     1981 acccgcagcg cacgcgcgtg gacttcaatt cccggtggct gtcgtcgatg acggctccgg
     2041 agttcgtcag gatagccgcg caatacacgg tggcccgcat gctcgagcgc gatgacttcg
     2101 ccaagcgtta tcgcgcgggt caggcgatct cgatccacga gtttctctac cccctggcgc
     2161 agggctatga ctcggtcgcg ttgcgagctg acgtcgaact cggcggaacc gatcagaagt
     2221 tcaatctgct cgtcgggcgg catctgcagg aggcgtacgg acaggagccc caggtcgtga
     2281 tcacgatgcc gctgctcgaa ggcaccgatg gcatcaacaa gatgtcgaag tcccagaaca
     2341 actacatcgg gatcgccgag gatccgacag taatgttcgg caagatcatg tcgatctccg
     2401 atgagctgat gtggcggtac ttcgagctgc tcagtttccg gccgctcggg gagcttgctg
     2461 cgttgcggcg cgcgacgcag gaggggcgga atccccgtga cgtgaagttc gagcttgcgt
     2521 ctgagatcgt gagccgtttc cacgacgctc aggctgcgga gcgcgcccag aaggagttca
     2581 tcgcccgggt cagcctcaag gaagtgccgg atgatctcga gcctctgacc gtcttcgtcg
     2641 atggcgccgg cctgagattg gcgaatgtgc tcaaagcagc cgggctggcg gccagcacct
     2701 ccgaggccaa tcgcaagata gatgaggggg cggtgcgaat agatggcacc cgcaccacgg
     2761 accggagcct gacgctggcg gccggtgcgg atcatgtgct ccaggtcgga tcgaggcgct
     2821 ttgcccgtct cagactggag ctaaagcctt gaataatcag gccggatagg gcctgagccg
     2881 agagtaggcc gggaaccgct agggggcgcg ccgctcaata cgcgtgccgg tttcgacgcg
     2941 catcatggca ctgcgttgac aaggcgccgc cggccgctat actgcgcgcc cctcccagac
     3001 cacgatcgtc atgcagggtc ctgccttgac cggcgccagc atgagctcga gcggttgccc
     3061 cgatggtggc cgcgaggttg acgggggacg ggccggggct atacttccgc cccctcgttt
     3121 tcccgctccc agcgtgcggg aatgctcttt aaaagtttgg caggtaattt gtgtggggac
     3181 tcgcgtcgat gcccttggtg catagaccga gtgaccaaga aagtccagca atgagtcttt
     3241 ctcgagccat tcgttttcga tgtttcaagt tcttcaagtg aagagtttga tcctggctca
     3301 gattgaacgc tggcggcgtg cctaacacat gcaagtcgag cggtaacagg tgtagcaata
     3361 catgctgacg agcggcgaac gggtgagtaa tgcttcggaa tctacccaat ggtggggaat
     3421 aaccagccga aaggttggct aataccgcat acgtcc
//
LOCUS       DASRHN010000058         8659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2328916, whole genome shotgun sequence.
ACCESSION   DASRHN010000058 DASRHN010000000
VERSION     DASRHN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328916"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFB37_02230"
     CDS             complement(<1..880)
                     /locus_tag="VFB37_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO21292.1"
                     /translation="MRPEQETVLHSWCKQTDWDAPTIVGGTGARLRTADGRAILDMSS
                     LAECSNLGHQHPRVVAAIRSQAERLCFVTNAWGAQPRARLAELLLEKSGFTGGRVFFT
                     LGGADANEHAIKFARQVRGQPRGWIVARDRSYHGASYAAMALSGDTRTAHQVDATAYR
                     VKHVAPPYAYRCPFGTADESACGSAAVDATAAAIDTLGAGDVAAVLMEPHAGTNGIVA
                     PASFWPALRSATRAKGVLLIADEVMSGFGRCGEWFAWQKYGEAGRPDIMTLAKGLTGG
                     HLPLGAVLVSAGIARAL"
     gene            992..2440
                     /locus_tag="VFB37_02235"
     CDS             992..2440
                     /locus_tag="VFB37_02235"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZO21293.1"
                     /translation="METAYETQLALRIDGERIVPGSRATHAVLNPATGSTLGELPLAT
                     PADLDRALDTAARGFRLWRSKSADERANVLAGAARLLRERISRIATTATLEEGKPLGE
                     TRAEVNAAANLFDFYAGETKRVYGRVLVRPAGMLVRVMKEPVGPVAAFCPWNFPIVNP
                     ARKLGAAIGAGCSIIIKPAEETPGSALEVVQCLLDAGLPKEAITCVFGVPDQVSRHLL
                     ASPVIRKVSFTGSVPVGKHLMKLAAEGGQRTTMELGGHAPVLVFDDADLGRALDILVT
                     AKFRNAGQVCVSPTRFYVQEGIYERFLKEFAARAAKLAVGNGLESGVQMGPMANPRRP
                     EAVEGFVSNAVQAGARVAAGGERGNGSTGHGGFFFRPTVLADVPLTARVMNEEPFGPI
                     ALMRSFKTLEEAVEQANRLPYGLAAYCFTENGRRALLVGDAIESGMVGINTTAIAAAD
                     APFGGVKDSGHGSEDGPEGLAACMVLKTIHQA"
     gene            complement(2467..3663)
                     /locus_tag="VFB37_02240"
     CDS             complement(2467..3663)
                     /locus_tag="VFB37_02240"
                     /inference="COORDINATES: protein motif:HMM:NF020505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monodechloroaminopyrrolnitrin synthase PrnB
                     family protein"
                     /protein_id="HZO21294.1"
                     /translation="MLTSKRVEAFDHWIRTAFVQMNTELENLYFAQQDRAKVQGVGDS
                     IKGAVRDEGHVHVDALLEEGNTGDGFDSAFGVLGNVGMYLGALRRHELTNPAREERSP
                     FVKASSLALHVGASLGMAPRFSTAHLATHNRAVAGVRKSFTSLPDEFLFIDENTRGIL
                     ALQQTADALTRIVQVGVSSPVADVLFETAAQGLRNVLRFNARLSELLDVQRFFYSVRP
                     YYKPYRVGRQEYRGANAGDFSGINEIDLLLGLCQANNPYYAQLLVDKMLFMLPADQAR
                     LRDCMMRVSLLDELLGLLEQHADTDWFQRNARAYLHLCDLFAQTAAQHHDTLVRRFIE
                     APAQQMSAEHLDGLTASGPPLPVLLRSLEVLRDLRTAADRADLPTRHADLARLRRHVR
                     GHDSST"
     gene            3974..5392
                     /locus_tag="VFB37_02245"
     CDS             3974..5392
                     /locus_tag="VFB37_02245"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO21295.1"
                     /translation="MPIKCRLPLIVLSIVAMQSATGAASGPVPPEADRELARSILAEL
                     VQIDTTHEHGSTRAAEIMRQQLQAAGFPSDDLVLLAPEDKPTKGNLIVRLHGRGRGRP
                     ILYLGHLDVVEARREDWSSDPFELLQKDGWFYGRGTEDMKDGDAALMETLIRLKREHF
                     RPARDIIVAFTADEEAGGDANGPAFLLREHRDLIDAEMVLNLDGGGGALQNDERLFFS
                     VGTSEKVYVTFTLETTSPGGHGSLPGPDNAIYRLAHGLTRLEALRFPVMMTDTTRASF
                     RQLAPLHSGAEQADMLAVAGSPPDPGAAERLSQTVRLNAQLRTTCVATLISGGHAENA
                     LPQRAKATIQCRMMPGDTAQNVQALLVSTLDDSAIHVSLDAPPILSPESPLTAPVLNK
                     VTRVVHSMWPGVPIVPTMATGFSDGRQTRNAGMPTYDVSGLWNDVNDNRAHGRDERIS
                     VQAFDESVEYTYKLMKAFASAR"
     gene            complement(5498..5911)
                     /locus_tag="VFB37_02250"
     CDS             complement(5498..5911)
                     /locus_tag="VFB37_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21296.1"
                     /translation="MHSEGPLNRKPAIPSTLDPSETTSVVEAAALLLARAIQETRKPV
                     EDLGGALQLMSRTALAPEGLAEQLAVCVESLQFHDRLVQQLACARELLAGILSQKAPD
                     LGACGAGRWNAVLAAVRCGNGEMADAGARGTCELF"
     gene            6099..6764
                     /locus_tag="VFB37_02255"
     CDS             6099..6764
                     /locus_tag="VFB37_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO21297.1"
                     /translation="MNERTPTVYIVDDDDVSRRYFSAVLSGEQIQSRMVESAQAFLQS
                     YDPREPGCLLLDVQMPGMNGLQLQQQLNLRGAVIPVIFVSGHAQVPTAVEALLQGAFG
                     FLQKPVPAEILLSQVRKALERDATNRAELREREKLTQRFHALTPREREVLEMLMAGRS
                     NKVMASDLSLSQRTVELYRARVMEKTGARSLAQLVRMAMELNIGPPSSRAPPSSAGHP
                     AGS"
     gene            6875..>8659
                     /locus_tag="VFB37_02260"
     CDS             6875..>8659
                     /locus_tag="VFB37_02260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO21298.1"
                     /translation="MHVRVLFVEDSEADAQLALRRLRADGMECSGRRVETEETLVGAL
                     REQAWDLILSDFSLPSFDGLSALTIAVRESPQVPFIFVSGTIGEERAIEALRCGAVDY
                     VLKSNLKRLAPAVRRALREASERVARDVAEARLRDVIDVARDWIWELDGQGIFVFSSD
                     SVRDVLGVQAAQIQGTHYSQWLHDGDRERMQAALAGLDRDSRTLGSLTARWLHADGST
                     RWLERHALALVNAEGAVTGFRGADRDVTEREAQQLRIIRLTRILEMLSGINTAVVRIR
                     DRMELLQEACRIAVTTGEYQTAVVSILDPGTRTARPRAWAGASNGITAEMAFTVAQTA
                     AGDTSITGRVLRSGELFLCNDVAQLDADVASWPTLRNITFRAVVGLPLEVDGTPVGVL
                     ILTAPDTETVTNDELRLLGEVAANLSFALQYFEKENAVQFLSYFDPLTGLAKRVLLCE
                     RLAARMAGLKSEERQPAVAVLDIEHLSIINDSYGRHAGDHLLQKIGDRLKGLFVDTER
                     LAYLGGGCFAIVLEDTGDQESALRELQQHTFALLQQPFRIGGHDIPVTVKSGLACRSA
                     GVDAQTLVQNAEVGLRQAKAAGEQYHPHQ"
BASE COUNT         1496 a         2801 c         2872 g         1490 t
ORIGIN      
        1 cgagtgcgcg ggctatgccc gccgagacca gcacggcgcc gaggggcaga tggcccccgg
       61 tcagaccctt ggcgagcgtc atgatatcgg ggcggcccgc ctcgccgtac ttctgccagg
      121 cgaaccactc accgcaacgc ccgaagccgc tcatcacctc atccgcgatc agcagcacgc
      181 ccttggcgcg cgtggccgag cgcaacgccg gccagaagct cgccggggcg acgatgccgt
      241 tggtgcctgc atggggctcc atgagcaccg ctgcgacgtc cccagcaccc agggtatcga
      301 tagcagccgc tgtggcatcc acggctgcgg agccacaggc gctttcgtcc gcggttccga
      361 aggggcagcg gtaggcatat gggggagcaa cgtgcttgac ccggtacgcg gtggcatcga
      421 cttggtgtgc cgtgcgcgta tccccggaca gcgccatggc ggcgtagctg gcgccgtggt
      481 acgagcgatc gcgcgcaacg atccagccac gcggctggcc ccgaacctgg cgcgcgaact
      541 tgatcgcgtg ctcattcgca tcggcgccgc cgagcgtgaa aaaaactcga ccgccggtga
      601 atccggactt ctccagcagc agttccgcca gtcgggcgcg cggctgtgcg ccccaggcgt
      661 tggtcacgaa gcacagccgc tcggcctgcg agcggatcgc tgcgaccacg cgcgggtgtt
      721 ggtggccaag attgctgcac tcggcgagac tgctcatgtc caggatggcg cggccatcgg
      781 cggtgcgcag ccgggctccg gtcccgccca cgatcgtggg tgcatcccag tctgtctgct
      841 tgcaccagct gtgcaggacc gtttcctgtt ccgggcgcat acgatcccgc ggcgtgggct
      901 gagcctcagt gaagcggcag tttaggggat attgaccggg gacgttataa tttgaggtcc
      961 ctcgcgactc tcaactttcc gggaaatcat catggagacc gcttacgaga ctcagctcgc
     1021 tctgcgcata gacggcgagc gcatcgttcc aggtagccgg gccacgcacg cggtcctgaa
     1081 tcctgccacg ggaagcactc tcggggagtt gcccctcgcc acgcctgccg atctcgatcg
     1141 ggcgctcgac acggccgcac gcggcttccg gctttggcgc agcaagtcag cggacgagcg
     1201 cgccaatgtg ctcgcgggcg ccgcccgact gctgcgcgag cgaatcagcc gaatcgccac
     1261 gacggcgacg ctcgaggaag gcaagccact cggcgaaacc cgggcggaag tgaacgccgc
     1321 ggccaacctg ttcgatttct atgccgggga gaccaagcgg gtttacgggc gcgtactggt
     1381 gcggccggcc ggaatgctgg tgcgggtgat gaaagagccg gtggggccgg tagcggcctt
     1441 ctgtccctgg aatttcccca tcgtcaatcc cgcgcgcaag ctcggggcag cgatcggcgc
     1501 gggctgctcg atcatcatca agcccgccga ggagacgccc ggctcggcgc tcgaggtcgt
     1561 gcaatgcctg ttggatgccg ggctgcccaa ggaagcgatc acctgtgttt tcggtgtacc
     1621 ggaccaggtc tcgcggcatc tgctcgcctc gccggtcatc cgcaaggtga gcttcaccgg
     1681 atcggttccg gttggaaagc atctgatgaa gctcgccgcg gagggcggcc agcgcaccac
     1741 catggagctc ggcggacacg ccccggtgct ggtgttcgac gatgccgatc tcggtcgtgc
     1801 cctggatatc ctcgtcacgg caaagttccg caatgccggt caggtttgcg tctcgccgac
     1861 tcgcttttac gtgcaggagg gcatctacga gcgtttcctg aaggaattcg ctgcgcgcgc
     1921 ggcgaagctg gccgtcggca atggactgga gagtggagtc cagatggggc ccatggccaa
     1981 cccgcgccgg cccgaggccg tcgagggatt cgtatccaac gccgttcagg ccggggcacg
     2041 cgtggccgcc ggcggcgagc gcggcaacgg ctcgacggga catggcgggt tcttcttcag
     2101 gccgacagtg ctcgccgatg tgccgctcac tgcccgcgtg atgaacgagg agccgttcgg
     2161 tccgatcgca ctgatgcgct cgttcaagac cttggaggag gccgtcgaac aggccaaccg
     2221 cctgccctac gggctcgcgg cctattgctt caccgagaac ggccgccggg cgctgctggt
     2281 tggagatgcg atcgaaagcg gaatggtggg catcaacacc accgcgatcg ccgcagccga
     2341 tgcacccttc ggaggggtga aggactctgg ccacggttcc gaggatggcc cggagggact
     2401 ggccgcctgc atggtgctca agaccattca tcaggcctga ggagaactcg ggtcgcggcc
     2461 gccgcgctaa gtgctcgaat cgtgcccccg gacgtgacgg cgcaaccggg ccagatcggc
     2521 gtggcgcgtc ggcaggtcgg cacgatcggc agccgttcgc aggtcccgca gcacctccag
     2581 cgaacgtaac agcaccggca gcggcggtcc gctcgcggta aggccatcga ggtgctccgc
     2641 gctcatctgc tgcgcgggag cctcgatgaa gcgcctgacc agggtgtcgt ggtgctgcgc
     2701 ggccgtttgg gcgaagaggt cgcagaggtg gaggtaggcg cgggcgttgc gctggaacca
     2761 gtcggtgtcg gcatgctgct cgagcagccc gagcagctca tcgagcaggc tgacccgcat
     2821 catgcagtcg cgcagccggg cctggtcggc cggcagcatg aagagcatct tgtccaccag
     2881 cagctgagcg tagtagggat tgttggcctg gcataatcca agcaacaggt cgatctcgtt
     2941 gatgcccgag aagtcacccg cgttggcccc gcgatactcc tggcgcccga ccctgtacgg
     3001 cttgtagtac ggccgcacgg agtagaagaa ccgttgcacg tcgagcaact ccgacaaccg
     3061 cgcattgaag cgcagtacgt tgcgtagccc ctgcgccgcg gtttcgaaca gcacgtcagc
     3121 gaccgggctg gacaccccga cctgcacgat gcgcgtcaat gcatcggccg tctgttgcag
     3181 ggccagaatc ccgcgcgtgt tctcatcgat gaacaggaac tcgtcgggga gtgaggtgaa
     3241 gctcttgcgc actcccgcta ctgcccggtt atgggtggcg aggtgggcgg tggaaaatcg
     3301 cggcgccatg ccgagcgagg cgcccacgtg caatgccagc gaggaggcct tgacgaatgg
     3361 cgagcgttcc tcgcgcgccg ggttcgtcag ctcgtggcgt cgcagggcgc cgagatacat
     3421 cccgacattc ccgaggaccc cgaatgcgct gtcgaatccg tcgccggtgt tgccttcctc
     3481 aaggagcgcg tcgacgtgca catgtccttc atcgcgcacg gctcccttga tcgagtcacc
     3541 aacgccctgg accttcgcgc gatcctgctg cgcaaagtag agattctcca gctcggtatt
     3601 catctgcacg aaggccgtgc ggatccagtg atcgaacgcc tcgactcgct tgctggtcag
     3661 catcgtctcg cctgatggtt ctcgtcgtga agcggaacat gccctcgcga gccgctattg
     3721 cgcaagcccg cgccgcctgc accattctga ccgcccgacg acggaacatt gttttgcgcg
     3781 ctcggcccat cagcttgcct cgacagaacg gacggaccgc acgggcccgc cctgaggagc
     3841 catccgtgct ggctgtctct tgagccgcgc acagctccgg gcatgcgagc cccaagggca
     3901 accgccccgg gggccgggct atactgcgcg ctcagcgccc tggtttcctc agcgcccgcc
     3961 gcaaagaggc tgcatgccca tcaagtgccg gttaccgctc atcgtcctca gcattgtggc
     4021 catgcagagc gcgaccggcg ctgcgtccgg tccggtcccg ccggaggcgg accgcgagct
     4081 ggcgcgctcc atccttgccg agttggtgca gatcgatacc acgcacgagc atggctccac
     4141 gcgtgccgcc gagatcatgc gccagcagct ccaagctgcc ggatttcctt cagacgacct
     4201 cgtgctgctc gccccagagg acaagcccac caagggcaac ctgatcgtaa ggctgcacgg
     4261 ccggggccgc ggcaggccga ttctgtacct cggccatctc gatgtggtcg aggcgcgacg
     4321 cgaggactgg tcgagcgatc ctttcgagtt gctgcagaag gatggatggt tctatgggcg
     4381 cggcaccgag gacatgaagg acggggacgc ggcgctcatg gagacgctga tccggctcaa
     4441 gcgcgagcac ttcaggccgg cgcgcgacat catcgtcgcc ttcactgccg atgaggaggc
     4501 cggtggggat gccaacggac cggcctttct gctgcgtgag caccgggacc tgatcgatgc
     4561 ggagatggtg ttgaatctgg acggcggtgg cggtgcgctg cagaacgatg agcggctttt
     4621 cttttcggtg ggcaccagcg agaaggtcta cgtcaccttc acactcgaga cgacgagccc
     4681 cgggggccac ggatccctgc ccggaccgga caatgcgatc tatcggctgg cccacgggct
     4741 gacacggctg gaggcattgc gcttccccgt catgatgacg gacaccacgc gagccagctt
     4801 cagacagctc gcgcccctgc acagcggcgc tgagcaggca gacatgctcg cagtcgccgg
     4861 ctcaccaccg gatccgggcg cggcggaacg tctgagtcag actgtccggc tgaacgcgca
     4921 attgcgcacg acttgtgtcg caacgctcat ctccggagga catgcggaaa acgccctccc
     4981 ccagcgtgcg aaggcgacca ttcagtgccg catgatgccc ggcgataccg cgcagaacgt
     5041 ccaggccttg ctggtgagca cactcgacga ttccgcgatt cacgtaagtc tcgatgcgcc
     5101 gccgatcctg agccctgaat ctcccttgac tgcgccggtg ctgaacaagg tgacccgtgt
     5161 cgtgcactcg atgtggcccg gtgtacccat cgtgccgacc atggcgaccg gcttcagtga
     5221 cgggcgtcag acccgcaacg cggggatgcc gacctacgat gtgagtggac tgtggaacga
     5281 tgtcaacgac aatcgcgcgc acgggcgcga tgagcgcatc tcggtgcagg ctttcgatga
     5341 gagtgtcgag tacacgtata aactcatgaa ggccttcgct tccgctcgat aaacggcctt
     5401 cgcttgatgc cccggcaatc gcagggcccc gggtcgcgcc ctgggtgcca gcatcggagc
     5461 tgatgtccga aaaatcgccg cggccatttt ccaatgacta gaacagttcg caggtccccc
     5521 gcgctccggc atccgccatc tcgccattgc cgcaacggac cgccgccagc acggcattcc
     5581 agcggcccgc gccgcaggca ccgaggtccg gcgccttctg cgagagaatg cctgcgagca
     5641 gctcgcgcgc gcaggccagc tgctgcacga gtcggtcgtg aaactgcagg ctctcgacac
     5701 acaccgccaa ttgctccgcg agaccctcgg gagccagtgc ggtccggctc atgagctgca
     5761 gcgcgccgcc gagatcctcg acgggcttgc gagtctcctg gattgcccgc gcgaggagca
     5821 gtgcggccgc ctcgacaacc gaagtcgtct cggacggatc gagcgtcgat gggatcgcgg
     5881 gcttcctgtt caggggtcct tctgagtgca tgcgggtccc tccatttcgt ccgtaagccc
     5941 tggtgcgccg ccgcatccgc aggtgcggat atttaatccg gggctgagct gcattatcgt
     6001 cggcctgctg cgggtcctgg agcactcgcg aggggaaccg cgatctgcgc acatccgtag
     6061 ttgccgcagt tgtcgcatac tctgttacga gggagcgcat gaacgaaaga acgccgaccg
     6121 tttacattgt cgatgatgat gacgtgagtc gccggtactt cagcgcggtg ctgtccggcg
     6181 agcagatcca gagccgaatg gtcgaatccg cgcaggcttt tcttcaatcc tacgatcccc
     6241 gcgagcctgg ctgcctgctg ctcgatgtac agatgccggg catgaatggc ttgcagctgc
     6301 agcagcagct caaccttcgc ggtgcggtga tcccggttat cttcgtcagc ggccacgctc
     6361 aggttccaac tgccgtcgaa gccctgctgc agggcgcatt cggatttctt cagaaaccgg
     6421 tcccggccga gattctgttg agtcaagtgc gaaaggcgct cgaacgggac gccacgaatc
     6481 gtgccgagct gcgcgagcgt gaaaagctga cgcagcgttt tcatgccctc acaccccgcg
     6541 agcgcgaggt gctggagatg ctcatggccg ggcgctccaa caaggtcatg gccagcgatc
     6601 tgagcctcag ccagcgcacc gtggagctct atcgcgcacg cgtgatggag aaaaccggtg
     6661 cccgctcgct cgcgcagctg gtgcgcatgg ccatggagct gaacatcggc ccaccctctt
     6721 cgcgcgcgcc gccgagcagc gccggtcatc ccgccgggag ttgacgcacg tctcatttgc
     6781 cgtgcagccc gggcactcca tgagcaacct cacagtgtac cggcgccgga aaatggtaac
     6841 ttcggccagc aaacgacgga gcgggtgctg aaaagtgcac gtcagggtgc tgttcgtaga
     6901 ggatagcgag gcagatgcgc agctcgcatt gcggcgcctg cgcgccgacg gcatggaatg
     6961 ctccggtcgg cgtgtcgaaa ccgaggaaac gctcgtcggg gccctgcgcg agcaggcgtg
     7021 ggatctgatc ctgtcggatt tttccctccc gagcttcgat gggttgtccg cgctgaccat
     7081 tgccgtacgc gagtcgcccc aggttccctt catcttcgtc tccggcacga tcggcgagga
     7141 gcgcgcgatc gaggccctgc gctgcggcgc cgtggactat gttctcaaga gcaatctgaa
     7201 gcggcttgcc ccggcggtgc ggcgcgcact gcgggaggcc tccgagcgcg ttgcacggga
     7261 cgtggcggag gctcggctca gggatgtcat cgatgtcgca cgcgactgga tctgggagct
     7321 cgatggccag ggaatcttcg tattcagcag tgattccgtg cgcgatgtgc tcggcgtgca
     7381 ggcggcgcag atccagggca cgcactacag ccaatggctc cacgatggcg atcgggagcg
     7441 catgcaggct gcactggcgg ggctcgatcg agactcgcgc acgctcggat cgctgaccgc
     7501 gcggtggctg cacgcagatg ggtccacgcg ttggctcgag cggcatgctc tggcgctcgt
     7561 gaacgccgag ggcgcggtaa ccgggtttcg cggagccgac cgggatgtca ccgagcggga
     7621 ggcccagcaa ttacgcatca tccgtttgac gcgcattctc gagatgctct cggggatcaa
     7681 caccgcggtg gtgcgcatcc gcgaccgcat ggagttgctg caggaggcgt gccgcatcgc
     7741 agtgaccacg ggcgaatatc agaccgcagt cgtctcgatc ctcgaccccg gcacccgcac
     7801 ggcacgcccg cgcgcctggg ccggagcatc caatggcatc acggcggaga tggcatttac
     7861 ggtcgcgcag actgcggcgg gcgatacgag catcaccggt cgcgtactgc gctcaggtga
     7921 gctcttcctg tgcaacgatg tcgcgcaact cgatgcggat gtggccagct ggcccacgct
     7981 gcgcaacatc actttccggg cagtcgtcgg tctgcccctc gaggtcgacg gaacgccggt
     8041 gggagtgctc atcctgaccg ctccagatac cgagacggtg acgaatgacg agctgagatt
     8101 gctcggcgaa gtggcggcca acctgtcctt cgcgctgcag tacttcgaaa aggagaatgc
     8161 ggtccagttc ctctcctact tcgatccact caccgggctc gcgaagcgcg tactgctgtg
     8221 cgagcggctg gcggcgcgca tggcgggcct gaagtccgag gagcgccagc cagccgtggc
     8281 ggtcctcgac atcgagcatc tgagcatcat caacgattcc tatgggcggc atgcgggcga
     8341 tcatctgctg cagaagatcg gcgatcggct gaagggcctg ttcgtcgata ccgagcgcct
     8401 ggcttacctc ggtggtggct gctttgccat cgtgctggag gatacgggcg accaggagag
     8461 tgcgctcagg gagctgcagc aacacacctt cgcactgtta cagcaaccct tccggatcgg
     8521 cggacatgac atccccgtga cggtgaaatc cggactcgcc tgtcgcagcg ccggcgtgga
     8581 tgcgcagaca ctggtccaga atgccgaagt cggcttgcgc caggcaaagg cagcgggtga
     8641 gcaataccat cctcaccag
//
LOCUS       DASRHN010000059         8004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2165701, whole genome shotgun sequence.
ACCESSION   DASRHN010000059 DASRHN010000000
VERSION     DASRHN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165701"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1302
                     /locus_tag="VFB37_02265"
     CDS             379..1302
                     /locus_tag="VFB37_02265"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HZO21299.1"
                     /translation="MPPSATTGAAAPDPDVTLFASPTRIDHIGRVVAPVMVNGRGPFR
                     FIVDTGATHSTISPALAARLGLQPSADASIEVNGVTGTARVPGVTIAKLQAGDLSIED
                     TRFPVVWAPLMAGADGILGAAGLQTQRLLVDFQHNRVIIYRASRRNTPAGFVRIPARR
                     LGGGLVTVEAIVGGVHVRAVIDTGSERSLGNMALLEALNARRRHGEQTRVARVYGATT
                     EVVSGDAQVAPTIVIDALRIADVTLVYGDFHIFKVWDMQAKPALIIGMDILGTVDALT
                     IDFHHADVFLASAAIRGTERAAAGWKVVGAQ"
     gene            complement(1426..2238)
                     /locus_tag="VFB37_02270"
     CDS             complement(1426..2238)
                     /locus_tag="VFB37_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HZO21300.1"
                     /translation="MRRRATALNCAALLLWVLSCGPGPALAQGGIPIYDYEVVHTYPH
                     DSHAFTEGLFYLNGFLYESTGLEQHSSIRRVRLETGEVLQKIDVAPQYFGEGIVNWKH
                     RLISLTWKSQVGFVYDLATFKMQKQFHYTGEGWALTQDGTHLIMSDGTSELRFLDPRT
                     LEESGRLKVTVAGRPLPSLNELEWVKGEIYANVWQTNVIVRIDPYSGAVLGVIDLANL
                     LPAADRTQGQTDVLNGIAYDAAHDRLFVTGKNWPKLFEIRLKRRAAAALVPK"
     gene            complement(2235..2828)
                     /locus_tag="VFB37_02275"
     CDS             complement(2235..2828)
                     /locus_tag="VFB37_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21301.1"
                     /translation="MHSILLVAAAVGIAAVPGPVSTPAGAPAAKPSHGCLASGNGYLR
                     ARIRGALSLDVDLHNSELECDGGARPDGSGIRVSFAGPLRSDGRRLRMVFGVGETSEG
                     QSGHALPTNLTVIFEGEQRLFATRGEDKCTVDQLTQERLGALGGPVRSYRVIARGFCI
                     APVHALKQNDAQESILVSSFDFAGRITFEDADEAPAK"
     gene            complement(2879..3793)
                     /locus_tag="VFB37_02280"
     CDS             complement(2879..3793)
                     /locus_tag="VFB37_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZO21302.1"
                     /translation="MSRPAPRSIEEYLKQLRAALEGADPALIQDALYDSEEYLRAEIA
                     AYPEKSEADVLELIATTYGAPEEVAAAYQATEAKVKEALRPPPPRAAASPSAWRRFFG
                     VYSDARAYTSLFYMLLTLATGIVYFVFVIAGLSMSAGLAVLIIGVPFFLAFVGIARVI
                     SLGEGRLLEAVTGERMPRRPVHPGAAAGWWARIIEMLRDPRTWSTLAYLVLMLPLGIV
                     YFVIATTGIALGGGLIVAPLVAAAQFFGWPFVEQATFHPEGLATGPGVLLCAALGVVI
                     FTALMHLARGVGRMQVAIARALLVKPGM"
     gene            complement(3790..4158)
                     /locus_tag="VFB37_02285"
     CDS             complement(3790..4158)
                     /locus_tag="VFB37_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZO21303.1"
                     /translation="MDDSGSRKFQKDLNAGLVALVLLAVLDQSGEDLYGYEIAKRLQR
                     ANQGEALFKEGTVYPVLRALSAGGLLTSRVVPSYAGPPRRYYRITDEGRTVLRNWSAI
                     WRQTRDFVDRFIETSPGVEA"
     gene            4378..6918
                     /gene="glgP"
                     /locus_tag="VFB37_02290"
     CDS             4378..6918
                     /gene="glgP"
                     /locus_tag="VFB37_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZO21304.1"
                     /translation="MLPGKLQRLPELTSNLFFSWHRPARALFEDLDPELWKQTGGNPR
                     LMLRCVGQSALDSAADDPVYLARYDQALQTLDAYLSAASTAVDAPLVGYFCAEYGFHE
                     SFQIYSGGLGVLAGDYCKAASDARANFVAVGLLYEQGYFTQTVDNDGIQHAEYAERDP
                     RDLPVEPARKASGEWVQVVVRIAARDVRARLWKAQVGRVPVYLLDTNCAENTPSDRDI
                     THRLYGGDESTRIRQEMILGIGGTRALRALGVAPAVWHLNEGHAAFLILELLRERLAE
                     GLPYNAALEAIAADCVFTTHTPVAAGHDAFSHDLIITHFSDFIRELGIPVERFLDLGR
                     SPSVPGLFNMTRLALNATRHVNAVSRIHAAVTSRLMADQWPEVRPEENAVGFVTNGVH
                     VPTFLHQKWADFFDHELGRDWRDRLRDREYWNALDQVPSDRYWTASQEVKARMLTSVR
                     ERLEREYARKGLSPAQLRHVTRFLDPMRPDVLTIGFARRFATYKRATLLLRDRPRLAR
                     LLRNPERPMIMLFAGKAHPADEPAKQVLREVRQLMTAPEFLGHVVFLEDYDLQLARWL
                     VSGVDVWLNNPIAPLEASGTSGIKAAINGRLNLSILDGWWAEGWMQDNGWGIPPANAQ
                     DPERRDALEAEMLFDTLEEEVIPLYYDRADSGYSPEWVRRSKRAMSTVIPRFNMDRVL
                     YDYTRGLYQPAARQFRRLAADGFAGARQLADWKQRVRQAWPKVSLRLLDDAAADLPRG
                     ARLKLKVAAGLNGLQPADVRVEFVARRLLPEADLGFPPLSAYGHDEPDGIWRATFTAT
                     GEVQSDGSAVFALDVEPKQCGQFATEVRIYPWHELLSHPNELGLMKWL"
     gene            7111..>8004
                     /locus_tag="VFB37_02295"
     CDS             7111..>8004
                     /locus_tag="VFB37_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZO21305.1"
                     /translation="MPRQPARASSGRYVSRLTGGTLAVIMAGGRGERLKDLTAHRAKP
                     ATPFGGKFRIIDFVLSNCVNSGIRQISILTQYKAQSLIQHVQRGWSYLRGEFAEFVEV
                     IPAQQQLGPVWYQGTADAVYQNIDLILSHRPKHVLVLAGDHIYKMDYGPMIAYHVEKG
                     ADITVGVVEVPAAESRNFGVLTATEWNRVTRFAEKPAVPDKLPGRPDSILASMGIYVF
                     NTRLLEKLLGADAAEGASSHDFGKDILPKAIGDNMQVFAYPFQDVETRAQSYWRDVGT
                     IDAFYDANLELVHVKPELNIYD"
BASE COUNT         1429 a         2570 c         2571 g         1434 t
ORIGIN      
        1 ttgttggtcg gccatgaggt caacatggag cggacggagt gggcacgatt cgaggtcttg
       61 atttggcagg gcacgcacgg ttcgcggccg aagtacgtgg tacctttcga caggagaccg
      121 gcccggcagg caggaaacga tcgaggaggt cgcttggcga ttctctggcg gctgagcctt
      181 gggctcgcag gatcactcgt cgcggctgtg gcgctggcgg cggtggaccc gagtgcggca
      241 tccgatccgc aggctgcgtt gcccgctgca caagcactcc cgtcctcacc agctccgagt
      301 ccaccagcag gcagcgccgc ttccgggagt tcgactcccg cagacccgcc tgcctcgatt
      361 caacctgtcg gcccgcagtt gccaccctca gctactacag gagcggcagc gcccgatccc
      421 gatgtcacgc tgttcgcttc ccccacgcgc atcgatcaca tcggacgggt cgtggctccc
      481 gttatggtga acggaagggg tcccttccga ttcattgtgg acaccggcgc gacgcactcg
      541 acgatctcgc cggcgctcgc agccaggttg ggcctgcaac ccagtgcgga tgcgtccatc
      601 gaggtgaatg gtgtgacggg cactgcacga gtgcctggcg tgaccatcgc gaagctgcag
      661 gcgggcgatc tgtcgatcga ggacactcgg ttccccgtgg tctgggcacc gctgatggcc
      721 ggagccgacg ggatcctcgg tgcagcgggc ctgcagacgc agcggctcct ggtcgatttc
      781 cagcataacc gcgtgatcat ctatcgcgcc tcgcggcgca acacgcccgc tgggttcgtg
      841 cgcattccgg cacggcggct cggcggtggt ctggtcaccg tcgaggcgat cgtcggcggc
      901 gttcacgtgc gcgcggtcat cgacaccggc tccgaacgca gtctcggaaa tatggcgttg
      961 ctggaagcgc tgaatgcacg gcggcgacat ggcgagcaga cccgtgttgc ccgggtctac
     1021 ggcgctacga cggaagttgt gtcgggcgat gcgcaggtgg cccccacgat cgtgatcgat
     1081 gcactgagaa ttgccgacgt gaccctcgtc tacggcgatt ttcacatctt caaagtgtgg
     1141 gacatgcagg cgaagccggc attgatcatt ggaatggaca ttctcggcac cgtcgatgcc
     1201 ctcacgatcg actttcatca cgcggatgtc tttctcgcga gcgcagcgat tcgaggtacg
     1261 gaacgagccg ctgcaggctg gaaggtggtc ggtgcccaat gacgagcaaa gcccgcggcc
     1321 gctgagtacc cggcttaggc cctgttagcg agggtcgcca ggccctgatg cgataggcgg
     1381 cgccgctgat cgccggccgc catttgggag cagaaaaccg cacccttact tcggcacaag
     1441 cgccgcggcg gcacggcgct tgaggcggat ctcgaacaac ttcggccagt tcttgcccgt
     1501 cacgaacaag cgatcgtgag cggcgtcgta ggcaattccg ttgagcacat cggtctgacc
     1561 ctgggtgcgg tccgcggcag ggagcaggtt tgccaggtcg atgacgccga ggacagcgcc
     1621 gctgtaggga tcgatccgaa cgatcacatt cgtctgccag acgttcgcat aaatctcgcc
     1681 cttcacccat tccagctcgt tgagactcgg cagcggccgt ccagcgaccg tgaccttcag
     1741 gcggccgctc tcctcgagcg tacgcggatc gagaaatctc agttccgagg tgccatcgct
     1801 catgatgagg tgcgtgccgt cctgggtgag cgcccagcct tcgcccgtat agtggaattg
     1861 cttctgcatc ttgaaagtgg ccaggtcgta gacgaagccg acctgtgact tccacgtcag
     1921 actgatgagg cggtgcttcc agttgacgat tccctcgccg aaatactgtg gcgccacgtc
     1981 gatcttctgc agcacctcgc ccgtctccag gcgcacgcgt cggatagagg agtgctgctc
     2041 gagcccggtg ctctcgtaga gaaaaccgtt gaggtagaac aaaccctcgg tgaatgcatg
     2101 gctgtcgtga ggataggtgt gcaccacttc gtagtcgtag atggggattc cgccctgcgc
     2161 cagtgccggg cctggaccgc acgacagcac ccacagcagc aacgccgcgc agttcaatgc
     2221 tgtggcgcgg cgcctcattt tgcgggcgcc tcatcggcgt cttcgaacgt aattcgcccg
     2281 gcaaaatcga agctgctgac caggatgctt tcctgggcat cgttctgttt gagtgcgtgg
     2341 accggagcga tgcagaagcc gcgcgcgatg acgcggtacg agcgaacagg tccgccgagg
     2401 gctccaagtc tctcctgcgt gagctgatcc accgtgcact tgtcctcgcc tcgtgtggcg
     2461 aacagccgct gctcgccctc gaagatgacc gtgaggttgg tgggaagggc gtggccagac
     2521 tggccttcgg aggtctcgcc gacgccgaac accatccgca ggcgccgccc atccgagcgc
     2581 aaaggcccgg caaagctgac ccggatccca ctgccatcgg ggcgagcacc gccatcgcac
     2641 tcgagctcgg agttgtgaag gtccacatcg aggctcaaag cgccgcggat ccgcgcgcgc
     2701 agataaccgt tgccgctggc gaggcagccg tgcgaaggct ttgcggcggg tgcgccggcg
     2761 ggagtggaga ccggacctgg gaccgccgct atgccgaccg ccgcggccac caggaggatc
     2821 gaatgcatcc gcgcagttta gcgtgagcgc tgagcgtgtg gcttctcacg agcgcagctc
     2881 acattccagg cttgacgagc agcgcccgcg caatcgcgac ctgcatgcgg ccgactcccc
     2941 gtgccaggtg catgagtgcg gtaaagatca cgacgccgag cgcggcacac agcagcacgc
     3001 cgggccctgt cgccagcccc tccggatgga aggtcgcctg ctcgacgaac ggccagccaa
     3061 agaattgcgc cgcggcgacc aggggcgcga cgatcagtcc gcctcccaga gcaatgcccg
     3121 tcgtagcgat cacgaagtac acgatgccca gcggaagcat caggacgaga taggcaaggg
     3181 tcgaccaggt gcgcgggtct ctgagcatct cgatgatgcg agcccaccaa cctgcggcgg
     3241 cgcccggatg cacagggcgc cgcggcatgc gctcgcccgt gaccgcttcg agcagccggc
     3301 cctctccaag tgaaatcacg cgggcgatac cgacgaacgc gaggaaaaaa ggtacgccga
     3361 tgatcagcac ggcaagtcca gccgacatgg acaggcccgc aatgacaaac acgaagtaca
     3421 cgatgccggt cgcaagcgtg agcaacatgt agaacaggga cgtgtaagcg cgcgcatcgg
     3481 aatacacacc gaagaagcgg cgccatgcgc tcggcgaagc cgcagcgcgt ggaggaggcg
     3541 ggcgcagggc ttccttgacc ttggcttcag tggcctgata tgcggccgcc acctcttcgg
     3601 gagccccgta ggtggttgcg atcagctcca ggacatccgc ctcggacttc tcgggatacg
     3661 cagcgatctc ggcacgcagg tattcctccg agtcgtacag ggcatcctgg atgagggccg
     3721 gatccgcacc ttcgagcgcg gcgcggagtt gcttcaggta ttcctcgatc gagcgagggg
     3781 cgggtcggct catgcttcaa ctcctgggct cgtttcgatg aagcgatcga cgaagtctcg
     3841 ggtctggcgc cagattgcac tccagttgcg caggaccgtc cggccttcat cggtgatccg
     3901 gtagtaacgg cgcggaggcc ccgcgtacga gggaaccacc cgactggtga gaagtcctcc
     3961 ggcactcagc gcgcgcaaca cggggtagac agtcccttcc ttgaagaggg cttcaccttg
     4021 attcgccctc tgcagccgct tggcgatctc atagccgtaa aggtcctccc cgctctgatc
     4081 gaggacggcg agcagcacga gcgccaccag ccccgcattg aggtccttct ggaacttccg
     4141 cgatccgcta tcgtccacag gccggtacta ttgactaact aatataggga tacaactaat
     4201 actatcggct ccagagaacc gttcgtcgca tttggatttg cgcctggtgc ccccacgcct
     4261 gcgccgggcc gctcctgcag cagtgagccc cgcggatccg actcgctgct agactgcgcc
     4321 ttttttgacg cccgcaagcg gaaacggatg agaaacccgc tacttgaagt gacccccgtg
     4381 ctcccgggga agctgcagcg cctgccggag ctcaccagca atctgttctt cagctggcac
     4441 cggccggccc gcgccctgtt cgaggacctc gatccggagt tgtggaagca gaccgggggc
     4501 aatccgcgcc tcatgcttcg gtgtgtcggc cagagcgctc tggattcggc ggccgacgat
     4561 cccgtctatc tggcgcgcta cgatcaggca ctccagaccc tcgatgccta tctttctgcc
     4621 gcctcgaccg cggtggatgc gccgctcgtc gggtacttct gcgccgagta cggctttcac
     4681 gagagcttcc agatctactc gggcggcctc ggggtcctgg ccggtgatta ctgcaaggca
     4741 gccagcgatg cacgggcaaa cttcgtggcg gtcgggctgc tgtacgagca gggctacttc
     4801 acgcagaccg tcgacaacga tggcatacaa catgcggagt acgccgagcg ggatccgcgg
     4861 gatctgccgg tcgaaccggc gcgcaaggcc agcggcgagt gggtgcaggt ggtcgtccgg
     4921 atcgcagccc gggatgtgcg cgcgcggctg tggaaggcgc aggtcggccg cgttccggtc
     4981 tatctcctgg acaccaactg cgcggagaac acaccatccg atcgggatat cacccatcgc
     5041 ctctacggcg gcgacgagtc cacccgcatc cgccaggaga tgattctcgg catcggcggg
     5101 actcgcgcgc tgcgggctct cggcgtcgcc ccggccgtct ggcatctcaa tgaagggcac
     5161 gcagcctttc tgatattgga attgctgcgc gagcggctgg ctgaaggact tccttacaat
     5221 gcggcgctcg aggcgattgc cgcggattgc gtattcacga ctcacactcc ggtcgccgcg
     5281 ggccacgatg ccttctcgca cgatctgatc atcacgcact tcagtgactt catccgcgag
     5341 ctcggcatcc ccgtcgagcg ctttctcgat ctgggtcgct cgccctcggt gccgggcttg
     5401 ttcaacatga cacgcctggc gctgaatgcc acccgtcacg tgaatgcggt gagccgcatc
     5461 catgcggccg tgacttcccg actcatggcc gatcagtggc cggaagtgcg ccccgaggaa
     5521 aacgcggtcg ggttcgtgac caacggcgtt cacgtgccca cgttcctgca ccagaagtgg
     5581 gccgatttct tcgaccatga gctggggcgc gactggagag atcgactgcg ggaccgcgag
     5641 tactggaacg cgctcgatca ggtcccgagc gatcgttatt ggacggcctc gcaggaagtg
     5701 aaggcgcgga tgctcaccag tgtgcgcgag cgtctcgagc gcgagtacgc gcgcaaaggc
     5761 ttgagcccgg cacagcttcg ccacgtcacg cgtttcctcg accccatgcg gcctgatgtg
     5821 ctcaccatag gcttcgctcg acggttcgcg acctacaaac gcgccacgct gttgctcaga
     5881 gaccgtccgc ggctggcccg cctgctgagg aatcccgagc gcccgatgat catgctgttc
     5941 gccggtaagg cgcatccggc cgatgagcct gcgaagcagg tcctgcgcga ggtgcgtcaa
     6001 ctcatgaccg cgccggagtt tctcggccac gtggtttttc tggaggacta cgacctgcag
     6061 ctcgcccgct ggctggtttc gggcgtggac gtgtggctca acaatccaat cgcgccgctg
     6121 gaggcaagcg gcacctcagg catcaaagcg gcgatcaacg gccggctcaa cctgagcatt
     6181 ctcgatggct ggtgggcaga gggctggatg caggacaacg gctgggggat cccgccggcc
     6241 aatgcccagg atcccgagcg ccgcgatgcg ctcgaagccg agatgctctt cgatacgctc
     6301 gaggaggaag tgattccgct gtactacgat cgcgctgaca gcggctattc acccgagtgg
     6361 gtccgccgca gcaagcgggc gatgagcacg gtcatcccgc gattcaacat ggaccgggtg
     6421 ctgtacgact acacgcgcgg cctgtatcag cccgcggcgc ggcaattccg caggctcgcc
     6481 gccgatgggt tcgcgggggc gcgtcagctc gccgactgga agcagcgggt gcgacaggca
     6541 tggcccaagg tcagcctgcg gctgctcgat gatgccgcgg ccgatctgcc gcgcggcgcc
     6601 cggctgaaac tgaaggtcgc cgccggactg aacggcttgc agcctgcgga cgtacgcgtc
     6661 gagttcgtgg cgcgccgcct gctcccggaa gccgatctgg gctttcctcc gctttcggcc
     6721 tacggccacg acgaacccga tggaatctgg cgtgccacct tcacagcgac cggtgaggtg
     6781 cagtccgatg ggtcggcggt gttcgcgctc gatgtcgagc ccaagcagtg cgggcagttc
     6841 gccaccgaag tgcgcatcta tccgtggcac gagctgctct ctcatccgaa cgagctcggc
     6901 ctgatgaagt ggctgtagtt cactgaaaaa gtcggttacg acgctttttc cgacgtgcct
     6961 tcgatagcga tcgcaaaagg tgcgattttt gcggcctgct gattccagtg ccacgactga
     7021 gcttcgcgat ttgagcacga gatccttgcg cgagcgcgct tcctgcggtg tagtacccgc
     7081 gtccccgatc ccgaaccctt ttgcgtcgcc atgcccagac agcccgcgcg agcctcctcc
     7141 ggacgctatg tcagccgcct gacgggcggc acgctcgcgg tcatcatggc gggcggccgc
     7201 ggcgagcgtc tgaaggatct caccgcccat cgtgcgaagc cggcgacgcc cttcggcggc
     7261 aagtttcgta tcatcgactt cgtgctctcc aactgcgtca actcgggtat ccgccagatc
     7321 tcgatcctca cgcagtacaa agcgcagtcg ctcatccagc acgtgcaacg cggctggagc
     7381 tacctgcgcg gcgagtttgc cgagttcgtc gaggtcatcc ccgcgcagca acaactcggt
     7441 cccgtctggt atcagggcac tgcggatgcc gtgtaccaga acatcgacct gatcctctcg
     7501 caccggccca agcacgtgct ggtgctcgca ggcgatcaca tctacaagat ggactatggg
     7561 ccgatgatcg cctaccacgt cgagaagggc gcggacatca cggtcggcgt cgtggaggta
     7621 ccagccgcgg agtcgcgtaa cttcggtgtg ctcaccgcca ccgaatggaa tcgcgtgact
     7681 cgctttgcgg agaagccggc cgtgcccgac aagttgcctg gacggccgga ttcgatcctc
     7741 gcctccatgg ggatctatgt attcaacacg cggctgctcg agaagctgct cggcgcagat
     7801 gccgccgagg gcgcctcctc acacgatttc ggcaaggaca tcctgccgaa ggcgatcggt
     7861 gacaatatgc aggtgttcgc gtatcccttc caggatgtgg agacgcgcgc ccagagctac
     7921 tggcgtgatg tgggcacgat cgatgcgttc tacgacgcca atctcgagct ggtgcacgtc
     7981 aagccggagc tgaacatcta cgat
//
LOCUS       DASRHN010000060         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1999748, whole genome shotgun sequence.
ACCESSION   DASRHN010000060 DASRHN010000000
VERSION     DASRHN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999748"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(782..1516)
                     /locus_tag="VFB37_02300"
     CDS             complement(782..1516)
                     /locus_tag="VFB37_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HZO21306.1"
                     /translation="MRSLFPVAIICVALAAPPASGAEVSGPAHIKDADTIEVRGSIFR
                     LHGIDAPELDQSCLDAEGALYACGRDAVRELRGLIAERTVICEDRGPDSAYPKRRIGR
                     CSVGGTDLHRWLVQNGWAVNFEPYAKGEFLADEEDARRGRLGIWKGCFVAPRDFRHWN
                     NGKGKLLGPECPQDARGKLFPDVADMLSGCEIKGHYSRRAWPYKGIYHLPGCRSYRRT
                     KAKRWFCSEEDAIAAGFRKAFTCDWH"
     gene            complement(2995..3189)
                     /locus_tag="VFB37_02305"
     CDS             complement(2995..3189)
                     /locus_tag="VFB37_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21307.1"
                     /translation="MQRGTPLAKLLKDGPDPITFFCRILADESAPEDKRREAARLLRP
                     YYHPLLAQMGPLLDRFGKED"
     gene            complement(3189..3521)
                     /locus_tag="VFB37_02310"
     CDS             complement(3189..3521)
                     /locus_tag="VFB37_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21308.1"
                     /translation="MAVSSTTWKKGKPKTGGRKKGTPNKVTLWHRQMLERMKVDGKDP
                     MSFAMSILQNEEAPFAERKWACEQLFPYSHPKLSSVEARVGGLTHEDRLERLRGMADR
                     VGLESETD"
     gene            complement(3585..4022)
                     /locus_tag="VFB37_02315"
     CDS             complement(3585..4022)
                     /locus_tag="VFB37_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZO21309.1"
                     /translation="MHGNIECAFIGRLGQEADLKTSAAGNPWTRLSLAVGQGDDTQWV
                     SVAVFGEAAERLCAGLRKGDRVYVEGTLRLAEWAGRDGHRRASLSVAAWKAEKLGAIG
                     RNRPARIKAGRSEGGEDESRHRQPAAYAAPDAPDGLHDAQIPF"
     gene            complement(4090..>5306)
                     /locus_tag="VFB37_02320"
     CDS             complement(4090..>5306)
                     /locus_tag="VFB37_02320"
                     /inference="COORDINATES: protein motif:HMM:NF023726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HZO21310.1"
                     /translation="EAERGARDDTSGQGRPLSLPEPVPSDDIVDGPELLHELGEAIGH
                     YVVLSPNEGVAVVLWVLHCYVFDAFTCTPRLAITSPEKQCGKTTLIDVLGCLVPRPLS
                     VSSITAAAIYRSVELARPTLLIDEADTFLHDNDELRGILNSGHRRGGEVVRLVGDGHE
                     PRQFSTHSPAAIAIIGQLPGTLADRSITIRMRRRLPDERVASFRSDRTDHLAEIARKA
                     ARWSIDNKDALRDADPDVGSLFNRVADNWRPLLAVADAVGGDWPQRARIAAAALSAAA
                     DEGSTRTMLLADIREYFRERDVDRVTSEDLVRHLNGLEGRPWAEFKAGKELTKNGLAR
                     LLKRFGISPASIRVPMGGTSKGYLAKDFTEVFERYLGPGRGFPTVTPSHPLVEAGHSN
                     FQSVTLNSSVTV"
BASE COUNT          862 a         1840 c         1674 g          930 t
ORIGIN      
        1 ccggccgtgc cacctgattg tcgcgcagcg tcaatcagcg cttgccaatc cggcggccaa
       61 cgacaatcat cgccataccc gaccgtcgat tctcatcctg attgacgcgc ggttctcatc
      121 cagattgtcg cgccatactt cctctcgcca ccaggcctcg gcggctcatg tgcactggtc
      181 gcgacggcat gggcaaccgc gagatggctg tcgcaaattc gggtcctggc ttctcttcgg
      241 ctcgatgctg cgctgcgagg gcatgcgcgc tcctggtcct tctgcgatcc tccttcggag
      301 atcgcgccga tccggtgccg gcttggcggc gccgcgtcct cgccgacggg ctttgtcaac
      361 ccgcttgttg gcacctcgcc gctcacccga tcgcgtccat ggcgcgacag acgagagtcg
      421 cgcggtccgg ccggctctcg tacgcgagcc atggcggcgt cccatcgttg gccaagcccg
      481 ctcacttcag cgggcaaaac cccgggggag catgctgccg gcacgtcgcg cagatcgtct
      541 tggcaaactc ccagccggcg aatgtgcagg aattgggctg gggacagcgc gcagccttct
      601 ctttgcagcc gacacacttg ccgcggctgt catccctatt gatccgccac cccgagccgc
      661 ccttgcagcc gcagccggag cagaaatgct gagcctccgc actgacgccg aagaaagcga
      721 gcagtccaag aacgagcaca gcaatgcgca tgacggcacc tccccgagca cacgacgcgc
      781 ctcaatgcca atcgcaggtg aatgccttgc gaaatccggc tgcgatggcg tcctcttccg
      841 agcagaacca gcgcttggct ttcgtgcgcc ggtagctacg gcagccgggc aggtgataga
      901 tgcccttgta cggccaggca cgccgcgagt agtggccttt gatctcgcag cccgacagca
      961 tgtctgccac gtcggggaat agcttgcctc tggcgtcctg cgggcactcg gggccgagca
     1021 acttgccctt gccgttgttc cagtggcgga agtcgcgagg cgcgacgaag caacccttcc
     1081 agatcccaag acggccacgc ctggcatctt cttcatcggc caggaattca cccttggcgt
     1141 agggctcgaa attcacggcc cacccgttct gcacaagcca gcggtgcagg tctgtgccac
     1201 ctaccgagca gcggccgatg cgccgcttgg gataggcgga atcggggccg cgatcctcac
     1261 aaatgacggt ccgctctgcg atgagccctc gaagttctcg aaccgcgtcc cgaccgcaag
     1321 catagagcgc gccttccgca tcgagacatg actggtcgag ctctggagcg tcgatgccgt
     1381 gcaggcggaa gatgctgccc ctcacctcga tcgtgtcggc atccttgatg tgtgccgggc
     1441 ccgacacctc cgctcccgag gctggaggag ctgcgagggc aacacagata atcgcaaccg
     1501 ggaacaatga acgcattgtc gctacctccc cgcgctccct gcaatgctgg tgtctgggcg
     1561 cgagcgctca atttgtgcac gcaaacgacg atgtagcaat agaggcccgt cagcaggaac
     1621 cgcgcggcgc cgaacacgta ggcaagggcc ggcggcagga aggtgcgcgg gctgccgggc
     1681 ggcagggaac cgagctcgat cgccccggcc gccagcgcct atgccgcgag cccgtccctg
     1741 cggccgggta gtcccagagg tgagccttct tgccgcgacg tgacgcggct gcacgtcgcc
     1801 tgggcaacgg agagggcttc ggctgaaccc gattggcacc gggattgcaa cccctgcggt
     1861 tggcgcccga cactggcatg cacgtggtga cgtggacgcc atgcccgcga cggagggcgt
     1921 gacaatccgg ccccccacga agcccatcga gagtgcggcc atccagagcg tgtccggctt
     1981 cggtaaatca cccactgagg tctgccgatc gcggtgctgc accgcggcag gccttcgagg
     2041 tcgcgcacga tgttgccggc atcgagtgcg accatgatct cttgcgtgct cacgcctcga
     2101 cgcggcgcgc gatcgccgcg gtcctgctca ggctcaggac cggcacttcg cggcacgccc
     2161 ccaccattct gccccgtcgg ctcgcaacgc gtcatcgcca cgcctccaaa gtaggtgaaa
     2221 atcgggggcg ccactgaagt agatcgaagt tggggtcgag tggatttgcg aggggggtcc
     2281 cgttcccacc cctccccttc gaaaatcttc aagggtaccc ccccctggcg cctggtcttg
     2341 gccacgagcg ggccagattg gccgacaaca cccggctttg gctctcgacc ggcgttcggg
     2401 cgcacaggtg cgctccacgt acttctgcct gcctcgaact tgcagcggca tgacgcgagc
     2461 gccggagagc gagccgacgt ggcggttgaa ggggaggcct acgtggaagg caggcgcatg
     2521 atcttcccgt ggtcgtcgga ggccacctct ttgcctgccg agtccgaagc cagccacgct
     2581 tgccagcagc cctatggccc atgccagcag cgtccttccg ccggaagggc ctccggcaga
     2641 tcgatgggcg ccaaacgggc ccgcgtctga agatggcgcc aaccgccacc ttgaggccgg
     2701 cctgtgaagc ccagtcgtcg tgctgctccc cagcacgggg ctgtggatga cgtcccggat
     2761 gccgggcgcc ggcggcaggg atgccaagcc ccggtgacag cgcaccgccc gcggccgcga
     2821 acgtggccgc caccaacgct tcgtccgcct cttgctcgaa catcggcgag gccacctcac
     2881 aaggcctcct cacgggccgc tggcgttccg cagcaatgag cagcaagccg caacaggccg
     2941 caaatccggc aacctcgccg ccgagacccg ctgttgggcg cacagagcac gacatcagtc
     3001 ctccttcccg aagcgatcga gcagcggccc catctgggca agcagcgggt ggtagtaggg
     3061 ccgcagcagc ctggccgcct cccggcgctt gtcctccggc gcgctctcat cggccaggat
     3121 gcggcagaag aacgtgatgg ggtcgggccc atccttcagc agcttcgcca gcggcgtccc
     3181 tcgctgcatc agtcggtctc gctctcgagg cctacgcgat ccgccatccc ccgaaggcgc
     3241 tccaggcggt cttcgtgcgt cagcccgccg acccgtgcct cgaccgagct gagcttggga
     3301 tggctgtagg gaaacagctg ctcgcatgcc cacttgcgtt cggcgaacgg cgcttcctcg
     3361 ttttgcagga tcgacatcgc gaaggacatc gggtccttcc cgtcgacctt catccgctcg
     3421 agcatctgcc ggtgccacag ggtcaccttg ttgggcgttc ccttcttcct gccaccggtc
     3481 ttcggcttgc ccttcttcca ggtcgtagag ctgactgcca tccctctatc ccctctaaac
     3541 accgtccagt aagtgctcct tcgaggccgt gcgggcccct cccctcagaa cggtatttgc
     3601 gcgtcgtgca ggccgtccgg tgcgtccggt gctgcgtacg ctgcgggctg ccggtgcctg
     3661 ctttcgtcct cgccaccttc gctgcgccct gccttgatcc ttgctggcct gttgcggccg
     3721 atggctccga gtttttcggc cttccaggca gcgactgaga ggcttgcgcg tctgtggccg
     3781 tcacggccgg cccactcggc gaggcgcagg gtgccctcga catagacgcg gtcgcccttg
     3841 cggagccctg cacacagccg ctcagcggcc tccccgaaga cagccacgga gacccattgg
     3901 gtgtcgtcgc cctggcccac ggcgaggctg agccgcgtcc aggggttgcc ggcggccgag
     3961 gtcttgaggt ccgcctcctg cccgagccgg ccgatgaagg cgcactcgat gttcccgtgc
     4021 atcgccccct cccctctttt gatccgtcac accgtcacaa gccttgagcc tcaagggatt
     4081 cagcggattt caaaccgtaa cactcgaatt gagtgttacg ctttgaaagt tgctatgtcc
     4141 ggcctccacc aagggatgtg acggtgtgac ggttggaaag ccccttcctg ggccgaggta
     4201 gcgctcgaat acctcggtga agtctttggc gaggtatccc ttggatgtgc ctcccatggg
     4261 aactcggatg ctcgccggtg atatgccgaa gcgcttgagg aggcgggcga ggccgttttt
     4321 cgtcagctcc ttgccggcct tgaactctgc ccaggggcgc ccctcgaggc cattgaggtg
     4381 tcggacgaga tcctcgcttg ttacccgatc cacgtcacgc tccctgaaat actcccgaat
     4441 gtcggcgagc agcatggtgc gcgtgcttcc ctcgtcggcc gccgccgaga gggctgcggc
     4501 ggcgatgcga gcgcgctggg gccaatcccc tcccaccgca tcggcgactg cgagcagcgg
     4561 gcgccagttg tccgcgacgc ggttgaacaa gctgccgaca tcggggtcgg catcccgcag
     4621 cgcatccttg ttgtcgatcg accaccgggc cgccttgcgt gcaatctccg ccaggtggtc
     4681 cgtccgatcg gaacggaacg acgccacccg ctcgtcaggc aagcgccgac gcatgcggat
     4741 cgtgatgctt cggtcggcca aggtgcccgg cagttgcccg atgatggcga tggccgcagg
     4801 tgaatgcgtg gagaactgcc gcggctcgtg cccatcgccc acgaggcgca cgacctctcc
     4861 gccgcggcgg tgcccactgt tcaatatccc gcgcagctcg tcattgtcgt gaaggaaggt
     4921 atcggcctcg tcgatgagca acgtcggccg cgccagctcc accgatcggt agattgccgc
     4981 tgcggtgatg ctggagacgg agagagggcg cggaacgagg cagccgagca catcgatcag
     5041 ggtggtcttg ccgcactgct tctccgggct cgtaatcgcc agacgcggtg tgcacgtgaa
     5101 ggcgtcgaac acgtagcagt gcaggaccca caggacgacg gccaccccct cgttgggcga
     5161 gagcacgacg taatggccga tggcctcgcc cagctcatga aggagctcgg gaccatccac
     5221 gatgtcgtcc gacggcaccg gctccggcag cgaaagcggc cgaccctgtc cgctcgtatc
     5281 gtcgcgggcg ccacgctctg cctcca
//
LOCUS       DASRHN010000061         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1999751, whole genome shotgun sequence.
ACCESSION   DASRHN010000061 DASRHN010000000
VERSION     DASRHN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999751"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFB37_02325"
     CDS             <1..222
                     /locus_tag="VFB37_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21311.1"
                     /translation="LLALLGLDWDDRCVQVPAARRIVRTASVWEVRRPLYTSSSGRAR
                     HYERELSALRAELAEPAVGGSGTCESTAG"
     gene            317..979
                     /locus_tag="VFB37_02330"
     CDS             317..979
                     /locus_tag="VFB37_02330"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HZO21312.1"
                     /translation="MFLQIEDFLSAAEVQTITEVAQKTKFIDGRRSNPHNMTKHNVIA
                     EPTDAAGQQAAQIALAALQRSDIARDFVFPRRVAIPVLTRYGVGMSYGAHIDSAFLPV
                     GTEPLRSDVSCTVFISDPSTYVGGELVIYLGSEEVRIKGKAGQAVFYASTSVHQVAPV
                     TSGERLVLVTFIESQIADPMHRDLLYALNEVRALEGLKMDWRNRTRLEYVSANLQRLW
                     SR"
     gene            1012..2643
                     /locus_tag="VFB37_02335"
     CDS             1012..2643
                     /locus_tag="VFB37_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO21313.1"
                     /translation="MERNRAADQATLHAVHAAAQRGAHTEAALLAQAALADGLEHPLL
                     LNVLALSHEQRGEPEEAARLLTRAVQIAPQDQPARNALGLCLLRLERPGRALVQFEAL
                     LQLNPSLPFAHVSRGTALAALGLIAEAEQSYQQALALDPQHAVALAGLASLASSRGAY
                     TDARSLAQSSLALLPGLPEAVLALAAAELGDQDVYGAEARIRALLADPRIGPLEHARA
                     NGLLGDILDAKNYTSEAFAAYSACNQELQKVFAGRFTGDQEAYAYVCSIQHYFEQVVA
                     ETWKQRPARDPRHTGAAQHVFLLGFPRSGSTLIEVILKGHRNIVTLQENESLIDALDE
                     FMRRPADLDGLIRAPETTLERLRAAYWHRVAAAGVDVMGKMFVDENPLNILKLPLIAR
                     LFPDARILFACRDPRDVVLSCFRHRFRMSASMYQLLTLAGAAGYYERVMQLGVQLFSL
                     LPLETYLVRHEDVVQGFAREMQRICAFLQIEWDPAMGDFALRTQKGAGLAPSATEMAK
                     ELNTEGIGQWRRYERQLAAVRPVLEPWVKRFCYEE"
BASE COUNT          489 a          886 c          862 g          490 t
ORIGIN      
        1 ctgctggcat tgctcgggct ggactgggat gaccgctgtg tgcaggtgcc ggctgcgcgc
       61 cgcattgtca ggaccgccag cgtgtgggaa gtccgccgac cgctttacac cagctcatcg
      121 ggccgcgccc ggcactatga acgagagttg tcggctctgc gggccgagct cgcagagccg
      181 gccgtcggag ggagcggaac ttgtgaatcc accgcaggct gagctcatcc gcgcgcacgg
      241 cccgcgccct acggggctcc tgccgtatca taggcgaccg gcccctcata ctgattgagc
      301 acagacggac tggaccatgt tcctgcaaat cgaagatttc ctgtcggctg cggaagtgca
      361 gacgatcacc gaggtcgccc agaagaccaa gttcatcgac ggccggcgat ccaatccgca
      421 caacatgacc aagcacaacg tcattgccga accgaccgat gctgccggcc aacaggcggc
      481 gcagatcgca ctcgccgccc tgcaacgcag cgacatcgct cgcgacttcg tctttccgcg
      541 gcgcgtggcc attccagtgc tgacgcgcta cggcgtgggc atgagctacg gtgcgcacat
      601 cgactcggct ttcctgcctg tgggcaccga gccgctgcgc tcggacgtct cgtgcacggt
      661 cttcatttcc gacccgtcca cctacgtcgg cggtgaactg gtcatttatc tcggctcgga
      721 ggaagtgcgt atcaagggca aggccggcca ggcagtcttc tatgcctcga cctcggtaca
      781 ccaggtcgct ccggtaacct ccggcgaacg tctcgtcctc gtcaccttca tcgaaagtca
      841 gatcgcagat cccatgcatc gcgatctgct gtacgccctc aacgaggtgc gagccctgga
      901 gggcctgaag atggattggc gcaaccggac gcgcctggaa tatgtgagcg ccaaccttca
      961 acggctctgg tcccggtagc cgatcagccg aatccgcgag aacgcaacgc catggaacgc
     1021 aatcgggccg ccgaccaggc gaccttgcac gccgtgcatg cggccgccca gcgtggcgcg
     1081 cacaccgaag cagccttgct ggcccaggcc gctctggcgg acggcctgga gcatccattg
     1141 ctgttgaatg tgctggcgct gagccacgag cagcgcggcg agcccgaaga ggccgcgaga
     1201 ttgctcaccc gcgcggtaca gattgccccg caggaccagc cggcgcgcaa tgccctcggg
     1261 ttatgtttgt tgcggctcga gcgccccggg cgggcgctcg ttcaattcga agcgctgctg
     1321 caactgaatc cctcgttgcc gtttgcgcat gtcagtcgcg gaacggcgct cgcggcgctg
     1381 gggctgatcg cagaggcgga acagagctat caacaggcac tcgccctcga tccgcaacat
     1441 gccgtggcgc tggcaggcct ggcgagcctg gcaagcagtc gaggcgcata caccgatgcc
     1501 cggtcgctgg cgcaaagctc cctcgccctg ttacccggct tgccggaagc ggtgctggct
     1561 ctggcggccg ccgagctggg ggaccaggac gtctacggcg ccgaagcgcg cattcgcgct
     1621 ttactggccg atccgcgaat cggacctctg gagcatgccc gtgccaatgg gctcctcggc
     1681 gatatcctgg atgccaagaa ttacacgtcg gaggctttcg cggcgtacag cgcctgcaat
     1741 caggagttgc agaaagtatt tgccggccgc ttcaccggtg atcaggaagc ctacgcttac
     1801 gtatgctcga tccagcacta cttcgaacag gtcgtagcgg agacctggaa gcaacgcccc
     1861 gctcgcgatc cgcgccacac cggggctgcc cagcatgttt ttctgctggg ctttccacga
     1921 tcgggctcca ccctgatcga ggtcatactc aagggacatc gcaacatcgt caccctgcag
     1981 gagaacgagt cactcatcga tgcgctggat gagttcatgc ggcgccccgc ggatctcgat
     2041 ggcctgatcc gggcaccgga aacgacactc gagaggttga gagccgctta ctggcatcgc
     2101 gtcgctgccg ccggtgtcga tgtgatgggc aagatgttcg tggacgaaaa cccactcaac
     2161 atcctgaagc tgccgctcat cgcacgtttg ttccccgacg cccggattct gttcgcctgc
     2221 cgtgatccgc gcgatgtcgt gctgagctgc ttccggcacc gattcagaat gagcgcctcc
     2281 atgtaccagc tgctgacgct ggcgggtgcc gccggctact acgagcgcgt catgcagctc
     2341 ggagtgcaac tgttcagcct gttaccgctc gagacctacc tggtcaggca tgaggacgtg
     2401 gtgcagggct ttgcgcggga aatgcagcgc atctgtgcat tcctgcaaat cgagtgggat
     2461 cccgccatgg gagacttcgc tttgcgcacc cagaagggcg ccggactcgc tccgtcggcc
     2521 acggagatgg caaaggagct gaacacggag ggcatcggcc agtggcgtcg ctacgaacgc
     2581 cagctcgctg ccgtgcggcc ggttctcgag ccctgggtca agcgcttctg ttacgaggag
     2641 tgaccctggg ctgtcgcgag atcctgctgc gctcgggccg gagtggaagc tcacgctgca
     2701 tcagggaggg caaaccaggt gttgaag
//
LOCUS       DASRHN010000062        14919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_516587, whole genome shotgun sequence.
ACCESSION   DASRHN010000062 DASRHN010000000
VERSION     DASRHN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14919
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516587"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFB37_02340"
     CDS             <1..772
                     /locus_tag="VFB37_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21314.1"
                     /translation="KDGYFWIMGRIDDVLNVAGHRLGTMEIESALVSHPRVAEAAVVG
                     KPHEIKGEAVFAYVVFRGERPTGDTGALVQELRNWVAEQLGAIAKPEEIRFADNLPKT
                     RSGKIMRRLLRSIARGEAITQDVSTLENPAILDQLRGVDAGAKATSPSAKKTAPAKEV
                     RGTGSARAKKAAHGAKAAHGAKAKRTARKSTSSGKLAGRKKRTGAVKAGSRRAGARGK
                     STRNRHVSRSKTTHAARKKTAAGRGRRATAKGSRGRKI"
     gene            795..1271
                     /locus_tag="VFB37_02345"
     CDS             795..1271
                     /locus_tag="VFB37_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO21315.1"
                     /translation="MSLKKPALEPLKVEPVSKTGYPEPYRSRVLPREKRPLGDAVGLK
                     SIGINRSTLLPGKESSMRHWHTHEDEFVFVLEGEVVLRTNGGEQVLMPGMCAGFPAGV
                     EDGHQLINRSSRPAVYLEISNRDDDDTVYYSDEDVDLVASPPHARGRMTRRDGSPI"
     gene            1326..1685
                     /locus_tag="VFB37_02350"
     CDS             1326..1685
                     /locus_tag="VFB37_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21316.1"
                     /translation="MGEVIHVAFGTEREWEQTRAKTADGLVSIGSLFGDDEELMRAKA
                     DCVYQILREIVEEVPAVQITTKMPENLSAEQLETLTRAIKEAALKGIEIAMTHSVQVL
                     MASIYDLCTSKLHACPQ"
     gene            complement(1679..2278)
                     /locus_tag="VFB37_02355"
     CDS             complement(1679..2278)
                     /locus_tag="VFB37_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZO21317.1"
                     /translation="MAVPASARLCTALCAIVYFGVATGADPGPATHYSLDPGKSRLDF
                     EFVQAGARNQGHFGQLAVSLDLAADNPAGGKLDVQVPTSSIDTGDKERDDTLKGADLF
                     DIAKYPQARFTSSQITRTANGYEAVGKLTLRGVTRDQRVAFVLRNAQEQGKAVTYLTG
                     KTTVKRLDFGIGQGDWKSTEWVGNEVLVSYSLRLVSTPH"
     gene            complement(2290..2847)
                     /locus_tag="VFB37_02360"
     CDS             complement(2290..2847)
                     /locus_tag="VFB37_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZO21318.1"
                     /translation="MTLRNTTTRWGGLAQSLHWLIVALLITQVILANIAMDLPIGMKK
                     VAMFARHKSVGLTILVLAIIRLLWRWTNPTPPLPNTLKPYERTLARLTHAALYLLLFA
                     MPLTGWMMTSARGFPVSWFNLFQLPDFVPKNQRLYEAMKETHHVLSYALYTVAFLHVA
                     AALKHHFVLKDDVLRRMLPTAKKPG"
     gene            2884..3672
                     /locus_tag="VFB37_02365"
     CDS             2884..3672
                     /locus_tag="VFB37_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZO21319.1"
                     /translation="MAWLPGGCALIVAASLLCGCRSGVLRPGTSHPAPPVTEQPAQAP
                     GALVPGHAGRPYSIIADESLLSILAFRGGALSKAGHNHVIAAHDLSGTVYVPENMLQT
                     SFELRFAVAQLKVDEPELRAREGADFPPDMPESAREGTRHNMLGPALLDAERFPEITL
                     LSEQLERAPGNSDSGDGSDGAAHAQARVRITVRGQSHSVSVPVRFQLQRDQLIADGEL
                     PLRQSELGLTPFSAMLGALQVQDEMRVKFHVVAKRAPVRGRDAN"
     gene            complement(3676..5685)
                     /locus_tag="VFB37_02370"
     CDS             complement(3676..5685)
                     /locus_tag="VFB37_02370"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HZO21320.1"
                     /translation="MWTFNDFPSGVVKQEYGADITPGWLDRVRQATVRLSNCTASFVS
                     STGLMLTNQHCVAACLDQHSGGGRSLLDDGFLARSRGAELSCGAQLADVLLETRNITE
                     QVRAAGRGLSEQAASEARKQTLSRLEQACEQDRARTRTGAPLKCETVALYDGGQYWLY
                     RYRRYTDVRLVFAPERDIAAFGGDPDNFRFPRWSLDFALLRAYQANGKPAQTPDYFEI
                     NPAGPDAGQLVFVSGHPGATERMLTVSELETLRDVDLPGWLLRAAELRGRYIQFGKSS
                     REAQRMVEAPLESLENAIKVHRVELEALLDETLLRRKRQEEAALRTRIAADPALARSV
                     GDPWATIAKAQQVERALSLPYTFLEEGAGFNSRLFNYARLLVRAAKERTKPSVQRLRE
                     YGDPALPRLEQQLGAALPVHPELEKLTLSFSLERMRERLGADEPLVRTLLAHDTPDSL
                     ATRLIEGSKLADPAVRLQLWNGGAEAVARSEDPMIDLARRVDPAAREIRKRYEAEVEA
                     PVSGAEQKIGQARFRLLGTSVPPDATFTLRLNFGTVQGWRENGEQIEPFTHLRRLFER
                     DTGEEPFRVPPSWLAAKDALDPDSKFDLSTNNDIVGGNSGSPLIAADGRLVGLVFDGN
                     LHSIASSFEFDPQLDRAVAVHPAIMLEALRTVYNATELLKELGWQ"
     gene            complement(5757..6479)
                     /locus_tag="VFB37_02375"
     CDS             complement(5757..6479)
                     /locus_tag="VFB37_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21321.1"
                     /translation="MPLGRFHEISLATPDIRASVEFYEALGFTQAPTMDVWPHPYGVL
                     TDGRLFIGLHQRDAPSPCVTFVMPDVAAHLSELARLGIELTVCRTAEEVFNEIGFLAP
                     CGQAVRGIEARTYSPVARDAAEVSLCGYFAEISVPARDFAAARTFWEPLGFVAAEQTE
                     SPFPRLALTSDHIDIAFHHPETFAAPLLVFRDRAMRERIARLRELALTLSSDVPRGLE
                     PSSAALLKSPEGMRLLLMEGED"
     gene            complement(6512..7588)
                     /gene="hemH"
                     /locus_tag="VFB37_02380"
     CDS             complement(6512..7588)
                     /gene="hemH"
                     /locus_tag="VFB37_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZO21322.1"
                     /translation="MRYVSSPDFHRDAAERIGILLVNSGTPASAAPADVRHFLAGLLG
                     DPRVVELPRLLWLPILYGLILPFRSHLSARRYRSIWTANGSPLLDLSQRLRTELIGTL
                     AQRMLAPLSLELGMLYGSEPSVPQALGRLRASGAQRILVVPMFPQYCGATTGAVYDRV
                     SEELRRWRWLPELRYVAEYHDHPGYIEALRASVIQHWEREGKTQHLLISFHGIPERHF
                     HDGDPYFCKCQKTARLLADELMLRESEWSVSFQSRFGPDDWLKPYTSTVLQDMPARGT
                     RQVTLVCPGFAVDCLETLEEIAIENRDRFLTAGGERFQYVPALNFRPEHARFLADLIA
                     RHCQGWTHVEFGLRATGAARGASA"
     gene            complement(7644..9101)
                     /gene="gabD"
                     /locus_tag="VFB37_02385"
     CDS             complement(7644..9101)
                     /gene="gabD"
                     /locus_tag="VFB37_02385"
                     /inference="COORDINATES: protein motif:HMM:NF008415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZO21323.1"
                     /translation="MKLKDPQLLRTQAYVNGQWIGAANGPAVEVFNPATREKLGTIPN
                     FGAAETRQAIEAAAAAFPAWAAKTAKERGAILRRWYELMMANQEDLATIMTAEQGKPL
                     AESKGEIAYAASFIEWFAEEGKRLYGDLIPAHQSDKRLLVLRQPIGVVAAITPWNFPA
                     AMITRKAGPALAAGCTFVCKPAQQTPYSALAMAELGARAGLPPGVLNLVTGKDATAIG
                     GEMTSNPIVRKMTFTGSTSVGKKLMAQCSGTLKKLSLELGGNAPFIVFEDADLDAAVA
                     GAIASKYRNTGQTCVCANRLLVQASVYESFTGKLVEAVSRLRVGDGLQGPTEQGPLID
                     SKALAKVEEHLADALSKGAHVVCGGKRHALGGTFFQPTVLTNVTPNMLVAREETFGPV
                     APLFRFSTEAEAIRMANDTEFGLAAYFYTRDLARSWRVSEALEYGIVGLNTGIISTEV
                     APFGGVKESGYGREGSRYGILDYTEMKYVCVGGIA"
     gene            complement(9118..9801)
                     /locus_tag="VFB37_02390"
     CDS             complement(9118..9801)
                     /locus_tag="VFB37_02390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZO21324.1"
                     /translation="MAEFTTIMARDGHEFQAYLAASSGKPRGAVLVIQEIFGVNAHIR
                     SVTEGFAAEGYTAIAPALFDRIRRGIELGYSPPELEEGAGYMKQLKLEDTLKDLAAAV
                     AVVRNSGRVGSVGYCWGGAMSYVAASELPIACAVVYYGRVANYLDRRPKCPVMYHFGT
                     QDRSIPLSDVDKVQAAHPQGVFHQYEAGHGFNCEPRETYNPQAAALARERTLQFFAHY
                     LAGAKQQPS"
     gene            9943..11229
                     /locus_tag="VFB37_02395"
     CDS             9943..11229
                     /locus_tag="VFB37_02395"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZO21325.1"
                     /translation="MLVGSAVRRVAIIGGVRIPFARAHSVYAKASNEDMLTAVFRAAV
                     ARFKLNGLRLGDVAAGAVIKHTKDYNLTRECVLSSGLDPQTPGVDLQRACGTSLEAAI
                     LIGNKIALGQIECGLAGGTDSISDAPIVYPRSYQQLLLESYRGRSTWQRLEPFLQLRP
                     RHFKPVLPAVVEPRTGLSMGQSTELMAKRWKITREDQDKLAFDSHVHAAAAWREGFYD
                     DLVVEFAGLKIDNNVRADTSMEKLAKLRPTFDPQAGTMTAGNSTPLTDGASAVLLGTP
                     EWAQRRNLPVLAYLTYGKVWAVDFASGHEGLLMAPAHAVPAMLRDANLTLQDFDYYEI
                     HEAFAAQVLCTLKAWESAEYCRDVLGLTAPLGSIERSKLNVKGGSIALGHPFAATGTR
                     IVGSLAKLLAGDANARRGLISACTAGGMGVTAILER"
     gene            11257..13059
                     /locus_tag="VFB37_02400"
     CDS             11257..13059
                     /locus_tag="VFB37_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZO21326.1"
                     /translation="MGMEGDLPEETVLLRANLGVVLSGLPLFNGLDEAIVGEIAAAAE
                     WLSLPGGATLFAAGEPPDALYVVLSGCLGAFAPDNMRDRRRFMGRVLSGDTVGEMGLI
                     SGRPRSAHVVALRDSELVRIPSESFNRIFGRHPEAMMRIARLTVDRLELSQSRVRGPR
                     HGARTFTLLPQSVEVDVGGFATEFVQALSRFGRTELVWSVRAESHTSHWFHKIETSNE
                     FVVYVGDPTPGRWSNLCVRQADALLLLARAEGEPGRWAALSSPRDSSMAPQRSELVLL
                     HDGALVRGAAARWLAGQPSVPHHHIVTGGDFARLARMLTGRGVGLVLSGGGARGFAHI
                     GIVKALREAGVPIDLVGGTSMGGILGAGVAQCWSVEELTHRFRRAFVEAKPLRDYTLP
                     FVSLVSGRKVSKLLRNDFGDITIEDLPLTFFCVSSNLTTGHSEVHRRGEVWRWLRASV
                     AIPGVLPPVMHKGEVLVDGGTMNNLPVDAMRELGRGPVIGCDVGADRAFTTDSDELDV
                     PLPWQLMSWLRTRRRRPNIFQILWRSGMVNSSAMTAAHREKTDLLLQPPLAQIDMLNW
                     LAFERAIASGYEYAVRRLEKLPADSPLRECMEPT"
     gene            complement(13091..13936)
                     /gene="mtnP"
                     /locus_tag="VFB37_02405"
     CDS             complement(13091..13936)
                     /gene="mtnP"
                     /locus_tag="VFB37_02405"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZO21327.1"
                     /translation="MIGIIGGTGLYQMEELRVTDSREVTTPFGAPSSPVMLGVIRDRN
                     VAFLARHGLHHTLLPGEINYRGNIWALKGLGVRTILGVSAVGSLREEIRPGDLALPSQ
                     YLDFTKGLRPATFFGRGLVAHVSTAHPSCRSTAELIAGAARSLNLPLHRDKTYACIEG
                     PRFGTRAESFFLRTAGADLVGMTNVPEAFLACEAQLGYCTIAVATDYDCWLEDAAHHV
                     SVEQVSSLFRSSLQRVQELLAAVVGQYTEDESRPCRRSLRSAIITPPDQMTSEQRELL
                     RFLSA"
     gene            14224..>14919
                     /locus_tag="VFB37_02410"
     CDS             14224..>14919
                     /locus_tag="VFB37_02410"
                     /inference="COORDINATES: protein motif:HMM:NF016174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21328.1"
                     /translation="MSKPGIDRVFERRLAALVNSGQPQILQGGRKGVEKESLRVLPNG
                     SLATSPHPRALGSALTNVHITTDYSESLIELVTPPFTASWELLQYLLDLHQFVYRHLG
                     EELLWATSMPCAIERDEDIPIAEYGSSHVGRMKTIYRRGLGLRYGRMMQAISGVHFNY
                     SFPVPFWELYADMRESRERGTDFISASYFELLRSYRRYGWIVLYLFGVSPVVCKSFLR
                     GRHLELTDLTGNTA"
BASE COUNT         2656 a         4793 c         4967 g         2503 t
ORIGIN      
        1 caaggacggc tacttctgga tcatgggccg catcgatgac gtgctgaacg tcgccggaca
       61 ccgcctgggc accatggaga tcgagtccgc cctcgtctca cacccgcgcg tggctgaagc
      121 cgccgtcgtg ggcaagccgc atgagatcaa gggcgaggcg gtattcgcct acgtggtctt
      181 tcggggcgaa cgtcccaccg gggataccgg cgcgctggtg caggagctgc gcaactgggt
      241 cgccgagcag ctgggtgcca tcgccaagcc ggaggagatc cgctttgcgg acaatctgcc
      301 caagacccgc tcgggcaaga tcatgcgccg cctgctgcgc tcgattgccc gcggcgaggc
      361 gatcacacag gacgtctcga cgctggagaa tcccgcgatc ctcgatcagc tgcggggagt
      421 cgatgcgggc gccaaagcca cgagcccgtc cgcaaaaaag accgcgccgg caaaggaggt
      481 gcgcgggact ggatcggcgc gagcaaaaaa ggcggcacac ggagccaagg cggcacacgg
      541 agccaaggcg aaacgtactg cgcgcaagag cacctcctcc ggcaagcttg ccggccgcaa
      601 gaagcgcacc ggcgccgtaa aggccggatc gcgcagagcg ggggcacgag gcaagtccac
      661 gcggaacagg cacgtcagcc gctcgaagac aacacacgcc gcgcgcaaga agactgcagc
      721 cggacgggga cgccgggcca ccgccaaggg cagccggggg cgcaagatct gaattcaaca
      781 taactggaga gcacatgtcg ttgaagaagc cggcgctcga gccactgaaa gtcgagcctg
      841 tcagcaagac cgggtatccc gagccgtacc gcagccgggt gttgcctcgc gagaagcgtc
      901 ccctgggaga cgcggtgggg ctgaagagca ttgggatcaa ccgctcgaca cttctgccgg
      961 gcaaggagtc ctcgatgcgc cactggcata cccacgagga cgagttcgtc ttcgtactcg
     1021 agggcgaggt cgtgctgcgc accaacgggg gtgagcaggt gttgatgcca gggatgtgtg
     1081 cgggctttcc ggcgggtgtc gaagatgggc atcagctcat caatcgcagc agccgacccg
     1141 cggtgtacct cgagatcagc aatcgcgacg acgacgacac agtttactat tccgacgagg
     1201 atgtggacct cgtggccagt cccccccacg cccggggccg catgacccgg cgagacggaa
     1261 gcccgatcta ggccgtatgt gccactccgc cttggatgcg gtacggaact tgcggattag
     1321 gccgcatggg tgaggtgata cacgtagcct tcggcaccga acgcgagtgg gaacagaccc
     1381 gcgccaagac cgccgacggt ctggtatcca tcggttccct gttcggcgac gacgaggagc
     1441 tgatgcgggc caaggcggac tgtgtctacc aaatcctgcg cgagatcgtg gaggaggtac
     1501 cggcggtcca gatcacgacg aaaatgcccg agaatctatc cgcggagcag ctcgaaacgc
     1561 tcacccgggc gatcaaggaa gcggcgttga agggcatcga gatcgcgatg acccactcgg
     1621 tgcaggtgct gatggcttcg atctatgacc tgtgcacctc gaagctgcac gcctgtcctc
     1681 agtgaggggt gctgacgagc cgtagcgagt acgaaaccag cacttcgttg cccacccact
     1741 cggtcgactt ccagtcgccc tgtccgatgc cgaaatccag acgtttgact gtcgtttttc
     1801 ccgtgagata ggtcacggct ttgccttgtt cctgggcgtt gcgcaggaca aaggcgacgc
     1861 gctggtctcg ggtcacacct cgtaaagtca gcttgcctac cgcctcgtag ccgttcgcgg
     1921 tccgggtgat ctgcgatgac gtgaagcgcg cctgcgggta cttcgcgata tcgaacaggt
     1981 cagctccctt cagtgtgtca tcgcgctcct tgtcgccggt atcgatcgag ctcgtcggca
     2041 cctgcacgtc gagctttcca ccggcgggat tgtccgctgc cagatcgagg cttacggcta
     2101 gctggccgaa atgtccctga ttgcgcgccc ccgcctgcac gaattcaaaa tccaaccggc
     2161 ttttgccggg atcgagactg taatgcgtgg cagggccggg atccgcaccc gtggcgacgc
     2221 caaagtaaac aatggcgcac agagcagtgc acagcctcgc gcttgcaggt acagccatcg
     2281 tgatcgacct cagccaggtt ttttcgctgt gggtaacatg cgccgcagga cgtcgtcctt
     2341 gagcacgaag tggtgtttca gtgccgccgc gacgtgaaga aacgccaccg tgtagagcgc
     2401 gtacgacaga acgtggtggg tttccttcat cgcctcgtac agccgttggt tcttgggtac
     2461 gaaatcggga agctggaaca ggttgaacca gctcaccggg aagccgcgcg cggaggtcat
     2521 catccagccg gtgagcggca ttgcgaacag aagcaagtaa agtgccgcgt gcgtgaggcg
     2581 tgcgagagtc cgctcgtaag gcttgagcgt gttaggcagc ggcggcgtcg ggttcgtcca
     2641 gcgccacagc agacgtatga tggcgagcac gaggatcgtc agcccgacgg acttgtgacg
     2701 cgcgaacatc gcgaccttct tcatcccgat cggcaagtcc atcgcgatgt tggccaggat
     2761 cacctgtgtg atcagcaatg cgacgatcag ccagtgcagg ctttgggcga gcccgcccca
     2821 gcgggtggtg gtgttccgga gggtcatgcg actaagctta agcggatgca cctgcgtttc
     2881 aaaatggcgt ggcttcctgg cggatgcgcg ctgatcgtag cagcgagcct gctgtgcggc
     2941 tgccgctccg gtgtcctgcg cccgggaacg tctcatccgg cgccgcctgt tacggagcaa
     3001 ccggcacagg cccccggggc gctcgtcccc ggacacgccg ggcggcccta cagcatcatc
     3061 gcggacgaat ctcttctgag catcctggct tttcgaggcg gcgcactctc caaggcagga
     3121 cacaaccatg tcattgctgc ccacgacctc tcgggaacgg tgtacgttcc cgagaacatg
     3181 ctgcagacct cattcgagct gcgcttcgcg gtggctcaac tgaaggtcga tgagccggag
     3241 ctgcgcgcac gggagggcgc cgattttccg ccggatatgc ccgaatcggc ccgggaagga
     3301 acacgacaca acatgctcgg cccggccctg ctcgatgcgg aacggtttcc ggagatcacg
     3361 ctactcagtg agcagctcga aagagctccg ggaaacagcg actctggcga cggctcggat
     3421 ggcgctgcgc acgcgcaggc gcgtgttcga atcaccgtcc gaggacagag tcactcagtc
     3481 agcgttccgg tgcgatttca actgcagagg gatcagctca tcgccgacgg cgagctgccg
     3541 ctcaggcaga gcgagctcgg cctgacgcct ttcagcgcaa tgctgggcgc tctgcaagtg
     3601 caggacgaga tgcgggtgaa gtttcacgtg gtggcgaagc gggctccggt acggggtcgc
     3661 gacgcgaact gacggctatt gccatccaag ctccttcagc agctcggtag cgttgtacac
     3721 cgtgcgcagc gcctcgagca tgatcgcagg atgaacggcg acggcgcggt cgagctgcgg
     3781 atcgaactcg aatgagctgg cgatggaatg caggttgcca tcgaacacca gccccaccag
     3841 cctgccatcg gctgcgatca gcgggctgcc cgagttgccg ccgacgatgt cattgttggt
     3901 ggatagatcg aacttggaat cgggatccag cgcatccttg gcggcgagcc agctcggcgg
     3961 cacccgaaac ggctcctcgc cggtatcccg ctcgaacagc cggcgcagat gcgtgaaagg
     4021 ctcgatctgc tcgccattct cccgccagcc ctgcacggtg ccgaagttca ggcgcagggt
     4081 gaaagtcgca tccggcggca cgctggtgcc caggaggcgg aagcgggcct gaccaatctt
     4141 ctgttcggcc ccgctcaccg gtgcctcgac ctccgcctcg tatcgcttgc gaatctcgcg
     4201 ggcggcggga tcgacgcgac gtgcgaggtc gatcatggga tcctcggaac gtgccacggc
     4261 ctctgcgccg ccattccaca gctgcaggcg tacggccggg tccgcgagct tcgagccctc
     4321 gatcagacgc gtcgcgagcg agtccggcgt gtcatgggcg agcagagtgc gcacgagcgg
     4381 ctcatccgct cccaagcgct cgcgcatgcg ctccagtgaa aaagacaggg tcagcttctc
     4441 gagctcggga tgcacgggga gcgcggcgcc gagttgctgt tcgaggcgcg gcagtgccgg
     4501 atcaccatat tcacgcaggc gttgcacgct gggcttggta cgctccttgg cggcgcgtac
     4561 cagcagtcgg gcgtagttga agagacggct gttgaatccc gcaccttctt ccaaaaaagt
     4621 gtatggaagc gagagggcgc gctcgacctg ttgcgccttg gcaatcgtcg cccacgggtc
     4681 cccgacgcta cgggccaggg ccggatcggc cgcgatgcgg gtgcgcaggg cggcctcttc
     4741 ctggcgcttt cgtcgcagca gggtttcatc gagcagcgct tcgagctcga cccgatgcac
     4801 cttgatggca ttctcgagcg actcgagggg tgcctccacc atccgttgcg cctcgcggct
     4861 gctcttgccg aactggatgt agcgaccccg cagctcggcc gcgcgcagca gccagccggg
     4921 caggtccaca tcccgcagtg tctcgagctc cgacaccgtc aacatccgct cggtggcgcc
     4981 gggatgaccg gagacgaaca cgagctgacc tgcatcgggt ccggcgggat tgatctcgaa
     5041 gtaatcgggt gtctgcgcag gcttgccgtt ggcctggtac gcacgcagca gagcgaaatc
     5101 cagactccag cggggaaacc ggaaattatc cggatcgccg ccgaaggctg cgatgtcgcg
     5161 ctcaggggcg aacaccaggc gcacgtccgt gtatcgccgg tacctgtaga gccagtactg
     5221 ccccccgtca tagagcgcaa cggtctcgca tttgagcggc gctcccgtcc gagtccgcgc
     5281 tcggtcctgc tcgcatgcct gctcaaggcg gctcagggtt tgcttgcgcg cttcgctggc
     5341 ggcctgctcg ctcagtcctc gcccggcggc gcgcacctgc tcggtgatgt tccgggtctc
     5401 gagcagcacg tccgccaact gcgcgccgca gctcagctcg gccccgcggc ttctggccag
     5461 aaaaccatca tcgagcaaac tcctgccgcc gccggagtgc tgatccaggc acgcagccac
     5521 gcagtgctgg ttggtcaaca tgaggcccgt ggaggagacg aaggaggccg tgcagttgct
     5581 caggcgaacc gtcgcctggc gcactctgtc cagccatccc ggcgtgatgt ccgccccata
     5641 ctcctgcttc accaccccgg acgggaaatc gttgaacgtc cacatcccct catcgctcag
     5701 cgccggcccc gccgcgagtg ccagcagcga cagaaggagc caggcgtgtg ggcggctcag
     5761 tcctcgccct ccatcagcaa cagcctcatc ccttcgggtg atttcagcag cgcagccgag
     5821 gagggctcga ggccgcgggg cacatcggac gagagggtga gcgcgagctc acgcagccgg
     5881 gcgatccgct cgcgcatggc cctgtcacga aagaccagca ggggcgcagc gaacgtctcg
     5941 ggatgatgga aggcgatgtc gatgtgatcg ctggtgaggg cgagacgcgg gaaggggctc
     6001 tcggtctgct cggcggcgac gaaacccagc ggctcccaga acgtgcgcgc cgctgcgaaa
     6061 tcgcgcgctg gcacgctgat ctcggcgaag tagccgcaga gggagacctc tgccgcatcg
     6121 cgcgccaccg gggagtacgt gcgggcttcg attccgcgca cagcctggcc gcagggagcg
     6181 agaaagccga tctcgttgaa gacctcctcg gcggtccggc agactgtcag ctcgattcca
     6241 agccgcgcca gctcggacaa gtgtgcggcc acatccggca tcacgaacgt cacgcagggc
     6301 gacggggcgt ccctttgatg cagaccgatg aacagccggc cgtcggtcag cacgccgtag
     6361 gggtgcggcc agacatccat cgtcggcgcc tgagtgaagc ccagggcctc gtagaactcc
     6421 accgaggcgc ggatatccgg cgtcgcgagg ctgatctcgt ggaaccgccc gagaggcaac
     6481 tgcggagctc cgctgcgcga aaggggggga ctcaggccga ggcgccgcgg gcggcgccgg
     6541 tggcccgcag accgaactcg acatgcgtcc agccctggca atgtcgcgcg atcaggtcgg
     6601 ccaggaagcg cgcgtgctcc ggccggaagt tgagtgcggg tacgtactgg aagcgctcgc
     6661 ccccggctgt cagaaagcgg tcgcgattct cgatcgcgat ctcctcgagc gtctcgagac
     6721 agtccactgc gaagcccggg cacaccagcg tgacctggcg cgtgccgcgc gccggcatgt
     6781 cctgcagaac cgtgctggtg tagggcttga gccaatcgtc cgggccgaat cgggactgaa
     6841 agctcacgct ccactccgac tcgcgcagca tcagctcatc ggccagcaga cgcgccgtct
     6901 tctgacattt gcagaagtag gggtcgccgt cgtgaaagtg tcgctcgggg atgccgtgga
     6961 aggagatcag cagatgttgc gtcttgcctt cacgctccca atgctggatc acgctcgcac
     7021 ggagcgcctc gatatagccg ggatgatcat ggtattcggc gacatagcgc agctcgggca
     7081 gccagcgcca gcggcgcagc tcctcgctca cccggtcata gaccgcgccc gtcgtcgccc
     7141 cgcagtactg gggaaacatc ggcacgacga gaattctctg ggcaccggag gcccgcaatc
     7201 gcccgagagc ctggggcaca ctgggctcgg acccgtagag catccccagc tcgagagaca
     7261 agggcgcgag catgcgctgc gcgagcgtgc cgatgagctc ggtgcgcaac cgctgcgaca
     7321 gatcgagcag cggagagccg ttcgccgtcc agatgctgcg gtatctacgc gccgagaggt
     7381 gcgagcggaa cggcaggatg agcccataca ggatcggcaa ccacagcagg cgtggcagct
     7441 ccacgacccg gggatcaccg agcagccccg cgaggaagtg gcgcacgtca gccggcgcgg
     7501 cggaagcggg cgtacccgaa ttgaccagca ggatgccgat gcgctctgcg gcatcgcgat
     7561 gaaaatccgg agagctcaca tagcgcatgg gtgtgacgat accgcaagcc ggaggtgggg
     7621 gaccacctgt tgcgggaaag cgctcaggcg atgcccccca cgcacacgta cttcatctcg
     7681 gtgtaatcca gaattccata acgtgagccc tcgcgcccgt accctgactc cttcacacca
     7741 ccgaagggtg caacctccgt cgagatgatg cccgtgttga gtcccacgat gccgtattcg
     7801 agcgcctccg acactcgcca cgagcgcgcc agatcgcgcg tatagaaata cgcagccagc
     7861 ccgaactccg tatcgtttgc catgcggatg gcctcggctt cggtggagaa gcggaacagc
     7921 ggcgccaccg ggccaaaggt ctcctcacgc gcgacgagca tgttgggagt gacattggtg
     7981 aggaccgtcg gctgaaagaa tgtgccgccg agcgcatggc gcttgccgcc gcagaccaca
     8041 tgcgccccct tggagagcgc gtccgcgagg tgttcctcga ccttggcgag cgccttcgag
     8101 tcgatcagcg gtccctgctc ggtcggcccc tgaagtccgt cgccgacgcg cagcctggag
     8161 accgcctcga cgagttttcc ggtgaaactc tcataaacgg acgcttgcac gagcagcctg
     8221 ttggcgcaga cacaggtctg accggtgttg cggtacttgc tcgcaatcgc gcccgcaacg
     8281 gcagcatcga ggtccgcgtc ctcgaagacg atgaagggcg cgttgccacc gagctcgagc
     8341 gaaagcttct tgagtgtgcc ggagcactgc gccatcagct tcttgcccac cgacgtggaa
     8401 ccggtgaaag tcatcttgcg cacgatcgga ttggaggtca tctcgccccc gatcgcggtg
     8461 gcatccttgc cggtcacgag attgagcacg ccggggggaa ggccggcgcg ggctccgagc
     8521 tcggccatcg ccagtgccga gtacggtgtt tgttgtgcgg gcttgcatac gaacgtgcag
     8581 ccggcggcga gcgcaggacc ggccttgcga gtgatcatgg ctgcagggaa gttccagggc
     8641 gtgatggcgg cgaccactcc gatgggctgc cgcagcacga gcagacgctt gtccgactgg
     8701 tgtgctggaa tcagatcgcc gtacaggcgt ttgccctctt cggcgaacca ctcgatgaac
     8761 gaggccgcgt aggcgatctc gcccttggac tcggccaatg gcttgccctg ctcggccgtc
     8821 atgatggtcg cgagatcctc ctggttggcc atcatcagct cgtaccagcg acgcaggatg
     8881 gcgccccgct ccttcgctgt cttggcggcc caggcgggaa aggctgcggc ggcggcctcg
     8941 atcgcctggc gcgtctcggc agcgccgaag ttgggtatgg tgccgagttt ctcgcgcgtg
     9001 gccggattga agacctccac cgccgggcca ttcgcagcac cgatccactg gccgttgacg
     9061 taggcctggg tccgcaggag ctgaggatct ttcagtttca tggcgcttct tccgagttca
     9121 ggacggctgc tgcttcgccc cggccaggta gtgggcgaag aactgcaggg tacgctcgcg
     9181 cgccagcgca gccgcctgcg ggttgtaggt ctcacgcggc tcgcaattga agccgtgccc
     9241 ggcctcgtac tggtggaaga caccctgcgg gtgggcagcc tggaccttgt ccacgtcgct
     9301 gagcggaatg ctgcgatcct gggtcccgaa gtgatacatc acgggacact tcgggcgccg
     9361 gtcgagatag tttgcgacac ggccgtaata cacgactgcg caggcgatcg gcagctcgct
     9421 ggcggccacg tacgacatgg caccgcccca gcagtagccg acgctcccta cacgtccgga
     9481 gttcctgacg accgcgacgg ccgccgcgag atccttcagg gtgtcctcca gcttgagctg
     9541 tttcatgtag ccggcccctt cctccagctc aggcgggctg tagccgagct cgatgccccg
     9601 ccggatccga tcgaacagcg caggggcgat ggcggtgtag ccctcggcgg cgaatccctc
     9661 ggtcaccgag cggatgtggg cattcacgcc gaaaatctcc tggatgacga gcaccgcgcc
     9721 gcggggcttg ccgctggatg ccgcgaggta ggcctgaaac tcgtggccat cacgcgccat
     9781 gatcgtggtg aactcggcca tagccgcaat ccgtccttcg gtggggcatg tgagaatacc
     9841 gccccgggtc caggcaggga aagctgggcg ggagcggccg cgccgtggca ttcttttcgg
     9901 ggtcgcgcgc cgtagcacct taccgtaacg aggggagaac acatgctcgt cggctcagca
     9961 gtgagacggg tcgccatcat cggcggtgta cggattccct ttgcgcgggc ccattcggtc
    10021 tacgccaagg cgagcaatga agacatgctc acggcagtgt ttcgcgccgc cgtggcccgc
    10081 ttcaagctca acggccttcg actcggcgat gtcgcggccg gcgccgtgat caagcacacc
    10141 aaggactaca acctgacccg cgagtgtgtt ctgtccagcg gcctcgaccc gcagactccc
    10201 ggggtggacc tgcagcgcgc ctgcggaacg agtctcgagg cggccattct gatcggcaac
    10261 aagatcgcgc tcgggcagat cgagtgcggg ctcgcgggcg gcacggattc catcagtgat
    10321 gcgcccatcg tctacccgcg ctcgtaccag caacttctgc tggagagtta ccggggccgc
    10381 agcacgtggc agcgactgga gccgttcctg caactgcgac cgcggcactt caagccggtg
    10441 ctgccggcgg tggtcgagcc acggaccgga ctgtcgatgg gccagagtac ggagctcatg
    10501 gccaagcgct ggaagatcac gcgcgaggac caggacaagc tcgccttcga cagtcacgtg
    10561 cacgccgcag cggcctggcg cgaaggcttc tacgatgacc tggtggtcga gttcgccggc
    10621 ctgaagatcg acaacaacgt gcgcgccgac acgagcatgg agaagctggc gaagttgcgg
    10681 ccgactttcg accctcaggc cgggaccatg acggcgggca acagcacccc gctgaccgat
    10741 ggcgcgagcg cggtgttgct cgggacaccg gagtgggccc agagacgcaa tctgcccgtt
    10801 ctcgcgtatc tgacctacgg caaggtgtgg gcggtggact tcgcgagcgg ccatgaggga
    10861 ctgctcatgg ccccggctca cgcggtgccg gcgatgctgc gggacgccaa cctgaccctg
    10921 caggacttcg actactacga gattcacgag gcgtttgccg cgcaggtgct gtgtacgctc
    10981 aaggcctggg aatcggcgga atactgccgg gacgtcctgg gactgactgc gcccctgggc
    11041 agcatcgagc ggtccaaact caacgtgaag ggaggcagca tcgcactcgg tcaccccttt
    11101 gccgccacgg gcacgcgcat cgtcggctcg ctggccaagc tgctggccgg tgatgcaaat
    11161 gcccggcgag gattgatctc ggcgtgtacc gcaggaggca tgggcgtcac ggccatactg
    11221 gagcgctgat ccccgctggc gaggggcagc actgacatgg ggatggaggg agaccttccg
    11281 gaggaaacgg ttctgctccg cgccaatctg ggtgtggtgc tgagcggact gccgctgttc
    11341 aacgggctgg acgaagccat cgtgggcgag atcgccgcgg cggccgaatg gctctccctg
    11401 ccgggcggtg caaccctgtt tgccgctggc gagccgccag atgcgctcta cgtcgttctc
    11461 agcggctgtc tgggagcatt cgcccctgac aacatgcgcg accggcgccg cttcatgggg
    11521 cgcgtgctct ccggtgacac ggtcggcgag atgggactga tctccggccg gccccgctcg
    11581 gcccatgtcg tggcgctgcg cgacagcgag ctggtccgca tcccgtccga gtccttcaac
    11641 cgcatcttcg gacgccaccc ggaggcgatg atgcgcatcg cgcgcctcac ggtagatcgt
    11701 ctggagctgt cccaatcgcg cgtgcgtggg ccgcggcacg gagcgcgcac cttcacctta
    11761 ttgcctcaga gcgtcgaggt ggatgtcggc ggctttgcaa ccgaattcgt ccaggcgctc
    11821 agccgcttcg ggcggaccga gctggtctgg agcgtgcgcg ccgaatccca caccagccat
    11881 tggttccaca agatcgaaac ctccaacgag ttcgtggtgt atgtcggcga tccgacaccg
    11941 ggacgctgga gtaacctctg cgtccgccaa gccgatgctc tgctgctgct ggcgcgcgcc
    12001 gaaggcgagc cgggacgctg ggcggcactc tcgagcccac gggattcgag catggcgccg
    12061 cagcgctccg agctggttct tctgcatgac ggcgcgctcg tacgtggggc ggccgcgcgc
    12121 tggctggccg gtcagccctc cgtaccccat catcacatcg tgaccggggg cgactttgcg
    12181 cgcctggcca ggatgctgac cggtcgcggc gtcgggctcg tgctctccgg cggcggcgcg
    12241 cgcgggttcg ctcacatagg catcgtcaaa gcgctgcgcg aggcgggtgt tccgatcgat
    12301 ctcgtcgggg gcacgagcat gggcggtatc ctgggtgccg gtgtggccca gtgctggagc
    12361 gtcgaggagc tgacacatcg cttccggcgc gcgttcgtcg aagcgaaacc cctgcgggac
    12421 tacacccttc ctttcgtgtc gctcgtctcg gggcgcaagg tcagcaagct gctgcgtaac
    12481 gatttcgggg acatcaccat cgaggatctg ccgctcactt tcttctgcgt ctcgtccaat
    12541 ctcaccaccg gccattccga ggtccaccgc cgcggcgaag tgtggcgctg gttgcgggcc
    12601 tcggtagcca ttccaggcgt gctgccgccc gtcatgcaca aaggggaagt gctggtggac
    12661 ggcggcacca tgaataatct gcccgtggat gccatgcgag agcttggccg tggaccggtg
    12721 atcggctgcg acgtcggggc ggaccgggca ttcaccaccg acagcgacga gctcgatgtg
    12781 ccgctgccgt ggcagctcat gagctggctg cgcactcggc gccgccgccc caacatcttt
    12841 cagatcctgt ggcgctcggg gatggtgaac agcagcgcca tgacggcagc ccaccgcgaa
    12901 aagacggatc tgctgctgca gccgccactt gcgcagatcg acatgctcaa ctggttggca
    12961 ttcgagcggg ccatcgcctc cggctacgag tacgccgtgc ggcgcctgga gaagctgcca
    13021 gccgattcgc cgctccggga gtgcatggag ccgacctgac cggcgcgact gttcagcggc
    13081 tgtatgttcg tcaggcagaa agaaagcgca gcagctcgcg ctgctcggaa gtcatttgat
    13141 ccggtggcgt gatgatcgcg cttcgaaggc tccggcggca ggggcgcgac tcgtcctcgg
    13201 tgtactgccc cacgacggct gcgagcaact cctggacgcg ctgcagactg ctgcggaaga
    13261 ggctgctcac ctgctccact gacacgtgat gcgctgcatc ctccagccag caatcgtagt
    13321 cggttgcgac cgctatcgtg cagtagccca gctgcgcctc gcaggccagg aacgcctccg
    13381 gcacgttggt catgcccacc aggtcggcac cggccgtgcg caggaaaaag ctctctgcgc
    13441 gggtcccgaa gcgcggccct tcgatgcagg cgtaggtctt gtcccggtga agcggcagat
    13501 tgagtgaccg cgcggcgcct gcgatgagct ccgccgtact gcgacagctc gggtgagcgg
    13561 tcgagacatg cgccacgagt ccacggccga aaaaagtggc cgggcgcaac cctttggtga
    13621 aatcgagata ctgggaaggc agcgccagat cgcccggtcg gatctcctcg cgcaggctcc
    13681 cgacggccga gacgccgagg atggtccgga ctcccagacc cttgagcgcc cagatgttgc
    13741 cccgatagtt gatctccccg ggtagcaggg tgtgatgcag cccatggcga gcgaggaagg
    13801 cgacgttccg gtcccggatg acacccagca tgacaggcga agagggcgca ccgaagggcg
    13861 tggtcacctc acgcgaatcg gttacccgca gctcctccat ctggtagagg cccgttccac
    13921 ctatgatgcc gatcattggg aaatcctcga aaccctgcgc ccaggctgcc tgcggcgcgt
    13981 atctgcgccg attcgccgct ggccgccgca cagtggcatc gcggcccgcg acgcggcaag
    14041 atgcctgaaa acgctccacg atgccccaaa ctgccgccgc acaacaagcc gaaagatggc
    14101 atcccggtca atacctcgga atacggacat ccgcccgggg agctgcggcg ctagcaatgc
    14161 gcgggccctt ggcgcccagc ctcgcgtgtg cggcatccgg gcgcaggcgc tggtagccca
    14221 acgatgagca aacccggaat cgatcgcgtc ttcgagcggc gtttggcggc cctggtcaac
    14281 agcgggcagc cgcagatcct gcagggcggc cgcaagggcg tggagaagga atccctgcgc
    14341 gtgctgccga atggctcgct cgccacgagc ccgcatcccc gtgccctcgg ctcggcactg
    14401 acgaacgtgc atatcaccac agattattcc gaatcgttga tcgagctcgt gacgccaccc
    14461 ttcacggcga gctgggagtt gctgcagtat ctgctggatc tgcaccagtt cgtctatcgg
    14521 catctcgggg aggagctgct ctgggcgacg agcatgccgt gcgcgatcga gcgggacgag
    14581 gacattccga tcgccgagta cggcagctcg cacgtcggca ggatgaagac catctaccgg
    14641 cgcggtctgg gcttgcgcta cgggcgcatg atgcaggcca tctccggcgt gcatttcaac
    14701 tactcgttcc cggtcccttt ctgggagctt tacgcggata tgcgcgagtc gcgcgaacgc
    14761 gggaccgact tcatctccgc gagctacttc gagctgctgc gcagctatcg ccgatacggc
    14821 tggatcgtgc tgtatctgtt cggagtctca ccggtggtgt gcaagtcctt cttgaggggc
    14881 cggcacctgg aattgaccga cctcacgggg aacaccgcg
//
LOCUS       DASRHN010000063        15805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_351917, whole genome shotgun sequence.
ACCESSION   DASRHN010000063 DASRHN010000000
VERSION     DASRHN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351917"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFB37_02415"
     CDS             <1..544
                     /locus_tag="VFB37_02415"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZO21329.1"
                     /translation="SVEVGEHRPLERESALQLTLAQGISRAERMDWVIQKATELGVTR
                     IVPLLTERSVVHLDSGQARKKLQHWRSIAIAACEQSGRNHVPQIEEPGPLLDFLHTQT
                     AGSRLLLAPDADRRLVDIASAADPVTVLIGPEGGLDESERQAALSAGFVAVRLGPRVL
                     RTETAALSALAVLQHRLGDL"
     gene            541..1323
                     /locus_tag="VFB37_02420"
     CDS             541..1323
                     /locus_tag="VFB37_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21330.1"
                     /translation="MKYIVIALLALVLGGMIGRALWGAKPAQPDPVQVIVTQLKTHAV
                     IQHERQLAIWYRACPSVFGKTPQIFIAWPARVSYQLELADVKVDRDGSVIKVSTAAIH
                     PDEPAVPTDFMDYLSTTSIFTFANEQELVNHEIGKATPIARYLTTYFLARDPSLQGDF
                     VDELRRLVEHLAAALGVPFTRVEVEVPKPQVSWPKLPKIELCEGTMASVNGLPFAKFA
                     DGATIPIGFRPPQASHPDRSLTTVEAPKGIASVYGGSGASHP"
     gene            complement(1381..1854)
                     /locus_tag="VFB37_02425"
     CDS             complement(1381..1854)
                     /locus_tag="VFB37_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZO21331.1"
                     /translation="MAERVTEIYSLLVPLTEGRLIIPRACVAEVIGFHVPSEMTGAPP
                     WYIGTVPWNGKAVPLVSFEGACGQMIPPASGRTRIVILHALGTRVEGGYFGIISQGFP
                     QLVRVSSDVVRPDNSRVFADRAPIICQVRMVNETPLVPDLERLEEMIAEETTVSA"
     gene            complement(1859..8176)
                     /locus_tag="VFB37_02430"
     CDS             complement(1859..8176)
                     /locus_tag="VFB37_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZO21332.1"
                     /translation="MPEVASQTLDFVARELNVTLAEARTALENYVEQPDNVNLLERCA
                     QELHQVQGVLRVLEIYGAALLAEEMEQVASYLLATAAERKSQAESLDALMRAMVQLPS
                     YLERVLAGGRDLALVLLPLLNDLRAVRGSALLSEGTLLLLNLKSDKQAQPVAGPAGEP
                     PLTVEQWARRVRARFQVGLIGWIRGERIEQNLDILAAVAQKLEQIATRQPVFQLWWVT
                     GAVIEALQENGLEGGVSVKRLLGLGDREIRRLYEQGEARYAQSPPVELLNNLLYYVGR
                     AESSGPRVAAVRASFRLAELLPVDESIEQERENLSAPSVKLMQTVAAAIREDLSKVKD
                     VLDIFVRRGAGQPKELSSQVEMLRKIGDTLGVLGLGELRGNVQEQTERLEKVVQGSLQ
                     ADEPTLVEIAAALIAVEDRLDGQLVGLIRPKSAAEPEEGEDSEFQQVQSAVLRECILN
                     LARIKEAITQNVGGTLDAAGLDSWQELMRGLKAGLLMLGKARAVETVEGITSQLKRVM
                     QPGGHGLPPGFLDRLADAIVSVEYYMETLQAGRSDPWYMLDNAQACVQALESQQTPQV
                     PTVAPLEPSAFARTVQIPPPTFSPETVAGAGGVAPTLTQTRAATPTALETADPELLKL
                     FIEEAHEELARIKRFFPAWDQNPLERDSLITVRRSFHTLKGSGRMVGARELGEYAWSI
                     ENLLNRILDNTLSRTPTIVEVLREAVAALPELVEQLETGRAPRVDAVSIATRAHALAA
                     GRPAASRPIPAATEPAPSEAATDGHAAAGEPQTMPEPHAASPLAEHAESSANSAAPAA
                     ERTTAPKEPASSEAIAWDGEPSLSADQLELEPPPPHADGIEPIAAEERNDELGILSAD
                     LPAAALQDEFTATSERRSLDEAAIPEAEPEAGAPVWSSAGPLDLESPPGPGPEQAGVD
                     EQLAADSRASEPAPEQHSTSATDELSLIDETAVHLEKSSHPEQAEAPYLPPTQDDVLR
                     DIYARETGTHVATVRAYLQRESGLPEPHAIPEEVYRACHTLSGSSKMAQARHGIRLAE
                     PLDHWLRRVYSSGLGLQSQDMALLADCMSAMEAVVSHLDEATGYFQTHWGLQERIAHA
                     EQVFDQRLAAAAQAQNEAPVPAPAEELSDATADFDPEVAAIFTEEAMELIELSEGALS
                     DWRGEPASADYRSALKRPLHTLKGGARMAGITPMGDLSHELETLVMQVDNGSVAADEQ
                     VFEVIQASLDELARMREAVANGGRVAPARAMIARIQALSRPGVAAAMTREPEERMAER
                     TALPAPGREPAAAETAQPLERAGSALPASQASAPTAEISESAPSAQPTAGDSAAETPA
                     GPAWDSEIEAPTSADFAERADGFKGPQGSSPAALAAPSGTELPDTVVAPVSIEAFVEL
                     EQAESTSGSLASEILAGRYEEADEPTIAEEGEQSVAEQDEPSLAPQHEHDGAPEPEVA
                     ASDDSSPSSASTISVSVPLGRMSEPRAYDDSLLPPQAVPPGREPVVAAERQEMARVDA
                     DLLDQLLNISGEASIARSRLEQQLGSIDFNLGELSRTVTRLKEQLRSLEIETEAQILH
                     RHEDESGHRGDFDPLELDRYSSIQQFSRALAETANDVASIQQLLENLAKDTQNLLQQQ
                     ARTITELQNGLMRTRMVPFQRHVQRLARIVRQAATDTGKRVELVVEGASGELDRQVLE
                     RMLPPFEHMLRNAVVHGIEKPSERVAASKPETGRIVLELHREGAEVMVRLSDDGAGMN
                     LQAIREKGAALGLVPQGRNLSDEDAMQLILEPGFSTAGTITQQAGRGVGMDVVATEIK
                     RLGGALHMETKPGEGTSFTIRLPFTLAISHALVVRTGEEFYALPLPTVEGVLRLTKSE
                     VSAHLGREAAAFEYGGQKYRFQHLATFVGLEPSPLPEQDVTIPVVLVRAGEHSTGLVA
                     DELVGSREIVVKSVGPQVSSIRGISGATILGDGRIVIILDIGALVRAEWRGRAPALPL
                     LQKDRSDKRTLAMVVDDSITVRRVTQRLLERNGMRVVTARDGMDAVTLLQDNVPDIIL
                     LDIEMPRMDGYEVAAHVRNDPRLKDVPIIMITSRVGEKHRARAIELGVDDYLGKPYQE
                     AQLLDAIEPLVERRRAAQASGTFARLGEARS"
     gene            complement(8193..10253)
                     /locus_tag="VFB37_02435"
     CDS             complement(8193..10253)
                     /locus_tag="VFB37_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZO21333.1"
                     /translation="MATPPRLAAGQMLLLGAIALLVAASAISFMASRPKAVTNAVPPT
                     GPLYAIAIDAQSAAAGDQAALTDFRSQLQHLKDDAAANPAPAYTKDPRFARLLGNATA
                     VLEAQSALTDASSAARETRELVPKLLSEVAAAASGLSGPSLESATRALERFELRAQRL
                     QLDVAALASGAANPTQAAQRLAESSDYLGQVIQGLMGTSPALGLPRLTGAEGEKRLKR
                     LDSLYTDLNGAVRRAVTAAPALPAAQAAANSVTADARALASASEPSGTANAGGSAPVW
                     TPVLIAVAALVLVFVGLAGAWRLRRALDQQRLAVETQRKENDRNQQAILRLLDELSSL
                     ADGDLTVQATVTEDITGAIADSINYAVDALRGLVTTINQSAIQLDSATRQTQALSGHL
                     AKASGAQSKQIASATESAGGMATSAEEVSGNAERAADVARHSVEVAHKGGDAVRRTID
                     GMNTIRETIQETSKRIKRLGESSQEIGNIVELINDIAEQTNILALNASIQASMAGDAG
                     RGFAVVADEVQRLAERAANATKQIEVLVRTIQTDTNEAVVSMERSTTDVVGGALLAEN
                     AGAALEEIEQVSNQIASLVQNISASSRQQTGAAQNIARNMQILKEISAQTADSTHATS
                     AAIAKLAELSAGLRKAAAGFRLPGSAAEPVGNTSAFKRLDESKSPANVRQGATHASHA
                     VGGT"
     gene            complement(10259..10822)
                     /locus_tag="VFB37_02440"
     CDS             complement(10259..10822)
                     /locus_tag="VFB37_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZO21334.1"
                     /translation="MTEELTLMSLRDRPYELLAELERRGRAVSAQVSQEGSAGREWVG
                     VALRMAGDLYLVAREETREVLGVPTALTRVPGAKAWIKGLANVRGQLLPIIDLRQFLG
                     SGVTPVTRNTRILVVNHREIPAGLLVDEVLGFRRFAEGEFSADAPPTVARCERYLAGA
                     FRRATEQWPVLNLRAVLESTAFAEAAA"
     gene            complement(10884..11249)
                     /locus_tag="VFB37_02445"
     CDS             complement(10884..11249)
                     /locus_tag="VFB37_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO21335.1"
                     /translation="MPLILIVDDSPTELHVMQKALEKHGYRTAAAADGAEGVRLAREM
                     SPDLIFMDIVMPGMNGYQATRALANDPRTRTIPIIMVTSKGQETDRIWGLRQGAIDYM
                     VKPVSPDQLVAKAQATLSA"
     gene            complement(11254..11667)
                     /locus_tag="VFB37_02450"
     CDS             complement(11254..11667)
                     /locus_tag="VFB37_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO21336.1"
                     /translation="MRGVALNVTGNNTKLQGLKVLVIDDSKTIRRTAETLLAKEGCEV
                     FTAIDGFDALAKIADHQPDIVFVDIMMPRLDGYQTCSLIKHNKVFRSIPVIMLSSKDG
                     LFDRARGRIVGSEHYLTKPFTKDELLSAIETHIGR"
     gene            12085..13062
                     /gene="gshB"
                     /locus_tag="VFB37_02455"
     CDS             12085..13062
                     /gene="gshB"
                     /locus_tag="VFB37_02455"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZO21337.1"
                     /translation="MKRLGVVMDPIDAIKYAKDSTLAMLLAAQERGFELEYMEQSDLS
                     LRDGIAFGRVRALTVRADAQNWFTLGEPRLERLGALDALLMRKDPPFDTEYIYSTYIL
                     ERAEEQGTLVVNRPQGLRDMNEKVYTAWFPQCCAPTLITREMGDMDSFLREHGKIVCK
                     PLDGMGGRSIFVLERNDKNMNVVFETLTGYGSRFAIAQRYIPDIVDTGDSRVLLIDGE
                     PVPYALARIPTATDHRGNLAAGARGVGRPLNDRDRWLAAQIGPTLAAKGMLFVGLDVI
                     GGYVTEINVTSPTGIRELDRQFGIDIGAVLIEALERRLQASSGEVSAGA"
     gene            13069..13971
                     /locus_tag="VFB37_02460"
     CDS             13069..13971
                     /locus_tag="VFB37_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZO21338.1"
                     /translation="MTRRAPSLREGKAPVRDRLVTMLFLAALLHGVVILGLTFDASAG
                     GRDSAPGLKVLLVSDEVPEAERNDTATYLAQRTQLGSGNTTESVSPRNPASSVPVPQQ
                     DGVPEGNSLNRQADWAGHPDESVITTTAWKTDVHYQAEEGNAGTSPDQPLLLQAQSSA
                     QPGPEEDSGPAQLSGPKRDELWVTPDTRAATLAPYLDSWRRKVERVGTINYPSAARNS
                     GLHSNPVVEVGISSDGKLERMLIQRSSGDAELDAAALAILKLASPFDPFPPELAREHR
                     VLRFVYEYQFSGGRLQGGAVSTVP"
     gene            14003..14632
                     /locus_tag="VFB37_02465"
     CDS             14003..14632
                     /locus_tag="VFB37_02465"
                     /inference="COORDINATES: protein motif:HMM:NF001266.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZO21339.1"
                     /translation="MTEKESMGILGDEPASEGEEGPSGPVSGLTNQLLIAMPSLLDPN
                     FSQTVALICEHTDKGALGIVLNKPLPMRLSDVLTQMKLEPSSEAIASLPVLRGGPVQT
                     DRGFVLHRPGGQWDHTHKVSDSIQVTTSRDILAAMARGEGPVDAFIALGYAGWESGQL
                     EREIRENAWLSMPVDARVVFELPFEERWLGAWRLMGIDVDRLSLVAGHA"
     gene            14669..15115
                     /gene="ruvX"
                     /locus_tag="VFB37_02470"
     CDS             14669..15115
                     /gene="ruvX"
                     /locus_tag="VFB37_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZO21340.1"
                     /translation="MKRPDTVLAFDFGLKRIGLAAGETLAGSAAPRPAIPVMRGSPDW
                     SAIEREVRMWHPQVLVVGAPYNVDGTPGALAEAARAFAAELGSRFGLPINMVDERWSS
                     LEAATALKMRRASGARRRRIRREDIDSAAAAVILERWLAGEGRPHE"
     gene            15108..>15805
                     /gene="pyrB"
                     /locus_tag="VFB37_02475"
     CDS             15108..>15805
                     /gene="pyrB"
                     /locus_tag="VFB37_02475"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZO21341.1"
                     /translation="MSDSQIEQRRPDGSLRHLLTLENLPRREIERLLNKAESFVRPLG
                     ARPPTSNALAGVTVANLFTEPSTRTRVSFELAAKRLGAHVVNLEVQLSSRVKGESMLD
                     TLYTLQSLHVDVMVIRDAEPGVPALAAANVAPHVSVLSAGEAHVAHPTQGLLDALTVR
                     QHKKRFEGLSIAIVGDIRHSRVARSAHHAFSALGVSDLRIIAPAALMPEANEFSGCTR
                     HTTLDTGLLDADVI"
BASE COUNT         2593 a         5317 c         5134 g         2761 t
ORIGIN      
        1 atccgtggag gtcggcgagc atcgcccgct cgaaagggaa tccgccttgc agctgacgct
       61 cgcacaaggc atctcgcgcg ccgaacggat ggactgggtg atccagaaag cgaccgagct
      121 gggcgtgacc cggatcgtcc cgctgctcac cgagcgcagt gtggtccatc tggacagcgg
      181 gcaggcgcgc aagaagctgc agcattggcg ctcgatcgcc attgccgcct gcgagcaaag
      241 tggccgcaac cacgtgccgc agatcgagga gcccggccca ctgctcgact tcctccacac
      301 ccaaacggct ggatcgcggc tgctcctcgc cccggatgca gatcggcgcc tcgtcgatat
      361 cgcatcagcc gcagacccgg tgacggtgct catcggaccg gaaggcggcc tcgacgagag
      421 tgaacggcaa gccgccctgt cagccgggtt cgttgccgtg cgcttggggc cccgcgtgct
      481 gcgcaccgaa acggcggcgc tgtccgctct ggcggtcctg cagcacaggc tgggtgacct
      541 gtgaagtaca tcgtcatcgc gctgctcgcc ctcgttctcg gcggcatgat cggccgcgcg
      601 ctttggggtg cgaagcccgc ccagcccgat ccggttcagg tcatcgtcac gcagctcaag
      661 acacatgccg tcatccaaca cgagcggcag ctggccatct ggtatcgcgc ctgtccgagc
      721 gtgttcggca aaacgcctca gattttcatc gcctggcccg cgcgcgtgtc ctatcagctc
      781 gagctggcag atgtgaaggt cgatcgcgat ggaagcgtga tcaaggtcag caccgcggcc
      841 attcatcctg atgagcccgc cgtccccacc gacttcatgg actacctctc gacgacctcg
      901 atcttcacct ttgcgaacga gcaggagctc gtcaaccacg agatcggcaa agccaccccg
      961 atcgcgcgct acctaaccac gtatttcctg gcccgggatc cttctttgca gggcgatttc
     1021 gtggacgagc ttcgccgcct ggtcgagcat ctcgccgccg cactgggcgt accattcacg
     1081 cgggtggagg tggaggtgcc gaagccccag gtgagctggc ccaagctgcc gaagatcgag
     1141 ctctgcgagg ggacgatggc ttcggtcaac gggctgccgt ttgccaagtt cgccgacggc
     1201 gccaccattc cgatcgggtt ccggccgccg caggcctcgc atcccgatcg atcactcacg
     1261 acagtcgaag caccgaaggg aatcgcctcg gtgtatggcg gctcaggggc gagccatcca
     1321 taggcgaaga cggacacgcg gactcggctg cggctggacg tgccgactcg cctcgcgcgc
     1381 ctatgcgctg acggtggtct cctcagcgat catttcctcg aggcgctcca gatcgggcac
     1441 cagcggtgtc tcgttgacca ttcgcacctg acagatgatc ggtgcgcgat ctgcaaagac
     1501 ccgcgagttg tcgggacgca cgacatccga gctgacccgc acgagctgcg ggaaaccctg
     1561 cgaaatgatc ccgaagtagc cgccctccac ccgtgtgccc agcgcgtgca gaatcacgat
     1621 gcgggttcgg ccggaggcgg gcggaatcat ctggccgcag gcgccctcga aggatacgag
     1681 cggcacggcc ttgccattcc aggggaccgt gccgatgtac cacgggggcg cgcccgtcat
     1741 ctctgacgga acgtggaagc cgatcacttc ggccacacag gcgcgcggga taatcaaccg
     1801 gccctcggtg agcggcacga gcaggctgta aatctcggtg acgcgctcgg ccatgtcttc
     1861 agctccgggc ctcaccgagg cgcgcgaacg tcccgctggc ctgcgccgcc cggcggcgct
     1921 ccacgagggg ctcgatcgca tcgagcagct gcgcctcctg gtaaggcttg ccgaggtagt
     1981 catccacacc aagctcgatt gcccgcgcgc ggtgcttctc cccgacgcgc gaggtgatca
     2041 tgatgatggg tacgtccttg aggcgcggat cattgcgcac gtgagcggcg acctcgtaac
     2101 cgtccatgcg cggcatttcg atatccagca ggatgatgtc aggtacgttg tcctgcagca
     2161 gggtgaccgc gtccatgcca tcgcgagcgg tgacgactcg catgccattg cgctccagca
     2221 ggcgctgcgt cacccgccgc accgtgatgg agtcatccac gaccatcgcg agcgttctct
     2281 tgtcgctcct gtctttctgc agcagcggca gtgcgggagc gcgtccgcgc cattcggctc
     2341 taaccagagc tccgatgtcc aggatgatca cgatgcgacc atcgcccaga atcgtcgcgc
     2401 ccgagatgcc gcggatgctc gagacctgcg ggcccaccga cttgacgacg atctcgcgac
     2461 tgccgaccag ttcatcggca acgagacccg tggagtgttc accggcacgg accagcacga
     2521 ccggaatcgt tacgtcctgc tccggaagcg gcgaaggctc gagccccacg aacgtcgcca
     2581 gatgctggaa gcggtatttc tgaccgccgt actcgaacgc ggcagcctcg cgccccagat
     2641 gagcgctgac ttcgctcttg gtgaggcgca agacaccttc gaccgtgggc aggggcaacg
     2701 cgtagaactc ctcgccggtg cgtacgacca gcgcgtggct gatcgcgagg gtgaagggaa
     2761 gacgaatcgt gaaggacgtg ccctcgccgg gcttcgtctc catgtggagc gcgccgccga
     2821 gacgcttaat ctcggttgcg acgacgtcca tgccgactcc acggccggcc tgttgagtga
     2881 tcgtcccggc ggtcgagaag cccggctcga ggatgagctg catggcatct tcgtcgctca
     2941 gattgcggcc ctgagggacc aggcccagcg ccgcgccctt ctcgcggatc gcctgcagat
     3001 tcatgccggc cccatcgtcc gagagccgca ccatgacctc cgccccttcg cgatgcagct
     3061 cgagcacgat gcgtccggtt tccggcttgc tcgcagccac gcgctcgctg ggcttctcga
     3121 tgccgtgcac cacggcattg cgcagcatgt gctcaaaggg tggcagcatg cgctcgagca
     3181 cctgccgatc caactcgccc gaggcgccct cgaccacgag ctcgacccgt tttccggtgt
     3241 ccgtcgcggc ctgtcgcacg atgcgcgcca gccgttgtac gtggcgctgg aacggcacca
     3301 tgcgcgtccg catcagaccg ttctgcaact cggtgatggt gcgggcctgc tgctgcagca
     3361 gattctgcgt gtccttggcg agattctcga gcagttgctg gatactcgcg acatcgttcg
     3421 ccgtctcggc aagcgcacgc gagaactgct ggatcgagga ataccgatcg agctcgagcg
     3481 gatcgaagtc gccgcggtga ccggactcat cctcatgccg gtgcaggatc tgcgcctcgg
     3541 tctcgatctc gaggctgcgc aactgctcct tgaggcgtgt cacggtgcgt gacagctcac
     3601 ccaggttgaa gtcgatcgat ccgagctgct gctccagacg cgagcgcgcg atactcgcct
     3661 cgccggagat gttgagaagc tgatcgagca gatcagcatc cacacgcgcc atttcctggc
     3721 gctcggcggc gacgaccggc tcgcgtccag gcggtacagc ctgcggaggc agcagcgaat
     3781 catcgtaggc gcgcggctcg ctcatccggc cgagcggcac ggacacggaa atggtgctcg
     3841 ccgaagaagg gctcgagtca tcggacgcgg caacctccgg ctccggcgca ccgtcgtgtt
     3901 catgttgcgg agcgaggctc ggctcatcct gctctgcaac gctttgctcg ccttcttcgg
     3961 cgatcgtcgg ttcgtctgct tcttcgtatc ggcccgccag aatttccgat gccaggctgc
     4021 ccgatgtcga ctcagcctgc tcgagctcta cgaacgcctc gatcgagacc ggggcgacta
     4081 cggtgtcggg aagctcggtg cccgagggcg cagccaaagc tgccggcgac gaaccctgcg
     4141 ggcctttgaa tccgtctgcc cgctcggcga agtccgccga agttggagcc tcgatctcgc
     4201 tgtcccaagc aggccctgca ggtgtctcgg ctgcagaatc cccggctgtc ggttgcgcgc
     4261 tcggcgctga ctccgaaatc tcggccgtgg gtgcgctggc ctgcgatgca gggagtgcag
     4321 atccagctcg ctcgagcggc tgcgcagtct cagcggctgc gggctcgcgt cccggagcag
     4381 gcagcgctgt ccgctcggcc atacgctctt cgggctcgcg agtcatcgca gcggccactc
     4441 cgggcctcga cagtgcctgg atgcgcgcga tcatggcgcg cgccggcgcg acacgaccgc
     4501 cgttggccac ggcctcacgc atccgcgcga gctcatcgag gctggcctga atgacttcaa
     4561 acacctgctc gtccgccgca accgagccgt tgtccacctg catgacgagg gtttcgagct
     4621 catgactgag gtcgcccatg ggggtgatgc cggccatacg ggcaccgccc ttgagcgtat
     4681 gcaggggccg cttcagagcc gagcggtagt ccgcactcgc cggctcgcca cgccagtcgc
     4741 tgagggcacc ctcggagagc tcgatgagct ccatggcttc ctcggtgaag atcgccgcca
     4801 cttccgggtc gaagtccgcc gtggcatccg acagctcctc ggcaggggcc ggcacggggg
     4861 cctcgttctg cgcttgagcc gcggccgcca ggcgttgatc gaaaacctgc tcggcgtggg
     4921 cgatccgctc ctgcagcccc cagtgagtct gaaagtagcc ggtcgcctca tcgaggtgag
     4981 acacgactgc ttccatcgcg gacatgcagt cggcgagcag cgccatgtcc tggctctgca
     5041 gtcccagtcc gctgctgtag acccgacgca gccagtgatc cagcggctcg gcgaggcgga
     5101 tgccgtgccg ggcctgcgcc atcttcgagc tgcccgagag ggtgtggcag gcccgataga
     5161 cttcttcggg aatggcatgc ggctccggca gccccgactc ccgctgcagg taggcccgca
     5221 ccgtagcgac atgtgttccg gtctcgcgcg catagatatc gcgcagcaca tcgtcctgtg
     5281 tcggtggcag gtaaggcgcc tcggcctgct ccgggtgaga cgacttttcg agatgcacgg
     5341 ccgtctcgtc gatgagcgac agctcgtcag tcgcggaagt ggaatgctgc tccggggcgg
     5401 gctcggacgc tcgcgaatcg gcggcaagct gctcatccac gccggcttgc tcgggccctg
     5461 gcccgggtgg actctcgagg tccaaaggcc cggcggagct ccacaccggt gcgcccgctt
     5521 cgggctcggc ttccggaatg gctgcctcat ccagcgatcg gcgttctgaa gtggcggtga
     5581 actcatcctg caaagcggcc gccggcaggt ccgccgacaa gataccgagc tcatcgtttc
     5641 gttcttcagc agcgatcggc tcgatcccgt ccgcatgcgg cggaggaggt tcgagttcga
     5701 gttggtcggc actcaacgag ggctcgccgt cccacgcgat tgcctcgctg gaggccggct
     5761 ccttaggcgc cgtggtgcgc tcggcagccg gggcagcact gttcgcagag ctctcggcat
     5821 gctcggccag cggcgaggcc gcgtgaggct cgggcatcgt ctgcggctcg cctgctgctg
     5881 catgcccgtc ggtggcggct tcgctcggag cgggctcggt ggcggccgga atcggccgac
     5941 ttgcagctgg acgccccgca gccagcgcat gcgcgcgcgt cgcaatcgaa acggcatcga
     6001 cgcgcggcgc tctgcccgtc tcgagctgct cgacgagctc gggcagggct gccactgcct
     6061 cgcgaagcac ctccacgatg gtcggggtac ggctgagcgt gttgtccagg atcctgttga
     6121 gcaggttctc gatcgaccag gcgtactcgc cgagctcgcg tgcgccaacc atgcgcccac
     6181 tgcccttcag cgtgtggaag gagcggcgca ccgtgatgag tgaatcgcgc tcgagtggat
     6241 tttgatccca ggccgggaag aaccgcttga tccgggcgag ctcctcgtga gcctcctcga
     6301 tgaagagctt caacagctcc gggtcggccg tttcgagcgc ggtcggtgtc gcggcgcgtg
     6361 tctgtgtcag agtcggagcc acaccgccgg cgcctgcaac agtctcgggc gagaatgtcg
     6421 gaggcggaat ctgcacggtg cgcgcgaaag ccgaaggctc gagtggcgca accgtaggaa
     6481 cctgcggggt ctgctgactc tcgagcgcct gcacgcaggc ctgcgcgttg tcgagcatgt
     6541 accacgggtc gctgcgcccg gcctgcagcg tttccatgta gtactcgacg ctgacgatcg
     6601 catcagccag tcgatccaga aaaccaggcg ggagcccgtg gccgcctggc tgcatcacac
     6661 gcttgagctg gctcgtgatg ccctcaacag tctccaccgc acgcgccttg cccaacatga
     6721 gcagcccggc cttcaggccg cgcatcagct cctgccagct atcgagcccg gccgcatcga
     6781 gcgtcccgcc cacgttctgg gtgatggcct ccttgatacg tgccaggttc agaatgcatt
     6841 cacgcagaac ggcagactgc acctgctgga actcgctgtc ttcgccttcc tcaggctccg
     6901 cagcgctctt cgggcggatg agtccgacga gctgaccatc gagccggtcc tccaccgcga
     6961 tcagcgccgc ggcgatctcg accagagtcg gctcatcggc ctgcagcgaa ccctgcacga
     7021 ctttttcgag acgctcggtc tgctcctgca cgttgccccg cagttcgcca agaccgagga
     7081 cgccaagcgt gtcgccgatc ttgcgcagca tctcgacctg ggacgacagc tccttgggct
     7141 gtcccgcccc gcgacggacg aaaatgtcca gcacatcctt gaccttcgaa aggtcttccc
     7201 ggatcgccgc agcaaccgtc tgcatgagct tcacgctcgg tgcggacagg ttctcccgtt
     7261 cctgctcgat gctctcgtcc accggcagca actcggccag gcggaatgaa gcgcgcaccg
     7321 cggccacgcg cggacctgag gattctgcgc gtccgacgta atagagaaga ttgttgagca
     7381 actcgaccgg aggcgactgc gcataacgcg cctcaccctg ctcatacagg cgccggattt
     7441 cgcgatcccc aaggccgagg agccgcttca ccgacacgcc cccctccagg ccgttctcct
     7501 gcagagcttc gatgaccgcc ccggtcaccc accagagctg aaaaacaggc tggcgcgtgg
     7561 cgatctgctc gagcttctgg gccactgccg cgaggatgtc gaggttctgc tcgatgcgct
     7621 ccccgcggat ccagccgatg aggccgacct ggaatcgtgc tcgcacgcgg cgcgcccatt
     7681 gctcgacggt gagtggcggc tcgcctgcag gccccgcaac cggttgcgcc tgcttgtctg
     7741 acttgagatt gagcagcagc agcgtgccct cggacagcag cgcactgccg cgcacggccc
     7801 gcaggtcatt cagcagaggc agcagcacga gcgccagatc gcgtccgccg gcgagcaccc
     7861 gctccaggta gctgggcaat tgcaccatcg cgcgcatgag cgcatccagg ctctcggcct
     7921 ggctcttgcg ctcggccgcc gtcgcaagca ggtagctcgc gacctgctcc atctcctctg
     7981 cgagcagcgc ggcgccgtag atttccagca cccgcagcac accctgcacc tgatgcagct
     8041 cctgggcgca acgctcgagc aaattgacgt tatccggctg ctcgacgtaa ttctcgagag
     8101 ccgtgcgcgc ctcggcgagc gtgacgttca gctcgcgagc gacgaaatcc agcgtctggg
     8161 aagcgacttc cggcattcag gactcgcccg cgtcaggttc cgccgaccgc gtgcgaagca
     8221 tgcgtcgcac cctggcgcac gttcgcgggg gatttgctct catcgaggcg cttgaacgcg
     8281 ctcgtgttgc caacgggctc ggccgcggag ccgggcagcc ggaagccggc cgccgccttg
     8341 cgcaagccgg ccgacagctc ggcgagcttc gcgatggccg cggaggtcgc gtgcgtcgag
     8401 tccgccgtct gggcgctgat ttccttcagt atctgcatgt tgcgggcaat gttctgggcc
     8461 gcgccggtct gctggcgcga gctggcggag atattctgca cgaggctggc gatctgattg
     8521 gagacctgct cgatctcctc cagagcggcg ccggcgttct cggccagcag cgcgccgccg
     8581 accacatcgg tggtgctgcg ctccatcgag acgaccgcct cgttcgtgtc ggtctggatg
     8641 gtacgcacca ggacctcgat ctgcttggtc gcattcgccg cccgctctgc cagccgctgc
     8701 acctcatcgg ccacgaccgc aaagccccgg cccgcatcgc cggccatcga tgcctgaatg
     8761 gacgcattca gcgccagaat gttcgtctgt tcggcaatgt cgttgatgag ctcgacgatg
     8821 ttgccgatct cctgcgagct ctctccgagg cgcttgatgc gcttggaggt ctcctggatg
     8881 gtctcgcgga tcgtattcat gccgtcgatg gtccggcgta ccgcatcccc gcccttgtgc
     8941 gccacttcca ccgaatgccg ggccacatcg gccgcgcgct cggcattacc ggacacttct
     9001 tcagccgagg tcgccatccc gccggccgat tcggtcgccg aggcgatctg cttggactgc
     9061 gcaccgctcg ctttcgccag atggcccgag agggcctgcg tctgtcgcgt agcgctatcg
     9121 agttggatcg ccgactgatt gatggtggtc acgaggccgc gcagcgcatc caccgcatag
     9181 ttgatcgagt cggcgatggc gccggtgatg tcctcggtca ccgtggcctg caccgtgaga
     9241 tcgccatcgg ccaggctgga gagctcgtcc agcagcctca ggatggcctg ttggttgcgg
     9301 tcattctcct tgcgctgcgt ctccactgcg agacgctgct gatccagcgc tcgccgcagt
     9361 cgccaggctc ccgccaggcc cacgaagacg aggaccaaag cggcgacggc aatgagcact
     9421 ggggtccaga ccggcgcgct gcccccggca ttcgccgtgc cactcggctc ggaagccgac
     9481 gccagcgcgc gagcgtccgc cgtgacggag ttggcggcag cctgtgcggc cggaagcgcc
     9541 ggcgctgcag tgacggctcg gcggaccgcc ccattgagat ccgtataaag ggaatcgagt
     9601 cgcttgaggc gcttctcgcc ttccgcgccc gtgagccgcg gcaatcccag cgccgggctc
     9661 gtgcccatca gaccctggat gacctggccc agatagtcgg agctctccgc gaggcgctgt
     9721 gcggcctgcg tgggattggc cgctccggag gcgagcgcgg cgacgtcgag ctgcaggcgc
     9781 tgggcgcgca gctcgaaacg ctcgagcgcg cgggtggcac tctcgagact cggaccggac
     9841 aggccgctgg cggcggcggc cacttccgac agcagtttag gaacgagctc gcgcgtctcc
     9901 cgcgccgcac tgctggcatc cgtcagcgca ctctgagcct ccaagaccgc cgtggcgttg
     9961 ccgagcaggc gcgcaaagcg tggatccttg gtgtaggcgg gcgccggatt ggcggccgca
    10021 tcgtccttca ggtgttgcag ctgactgcgg aagtccgtga gcgcagcctg atcgcccgcc
    10081 gcagcgctct gggcatcgat ggcgatcgca tacaggggcc ccgttggcgg caccgcattc
    10141 gttacggcct tgggccggct cgccatgaag gagatcgcac tcgcggcgac gagcagcgcg
    10201 atcgccccga gcaggagcat ctgccctgcc gcgaggcgtg gcggtgtggc cacgttgctc
    10261 aagccgctgc ctcggcgaat gccgtgctct cgagcacggc gcgcagattg aggacgggcc
    10321 attgctccgt agcgcgccgg aatgctccgg ccaggtagcg ctcgcatcga gccacggtgg
    10381 gcggcgcatc ggctgagaac tccccttcgg cgaagcgccg gaaccccagc acctcatcga
    10441 cgagcagtcc ggccgggatc tcgcgatggt tgacgacgag gatccgggtg ttacgcgtca
    10501 ccggtgtcac gccgctgccc agaaactgac gcaggtcgat gatcggcaga agctgacccc
    10561 gcacgttggc gagtcctttg atccacgcct tggcacccgg aacacgcgtc agagccgtcg
    10621 gcactcccag cacctcgcgt gtttcctcgc gcgccaccag gtagaggtcg ccagccatgc
    10681 gcagcgccac gccgacccat tcgcgaccgg cagagccttc ctggctgacc tgcgccgaga
    10741 ctgccctgcc gcgccgctcg agctccgcga gcagctcgta cgggcggtcg cgcagcgaca
    10801 tcagggtgag ctcttcagtc attggatttc tcgtgcgcga cgcgccggga aggaagcctg
    10861 ttcggctgca tgagactcgt tcctcacgcg gacaacgtcg cctgggcctt ggcaacgagc
    10921 tgatcgggcg agacgggctt gaccatgtaa tcgatcgcac cctggcgcag tccccagatc
    10981 cggtcggtct cctgaccctt cgaggtgacc atgatgatcg ggatcgtgcg ggtgcgcgga
    11041 tcgttggcga gggcgcgcgt cgcctggtac ccgttcattc caggcatgac gatgtccatg
    11101 aaaatcaggt cgggactcat ctcacgcgcc agccgcaccc cttcggcgcc atcggctgcg
    11161 gccgcggtgc gatagccgtg cttctcgagg gccttctgca tgacgtgcag ctcggttggc
    11221 gagtcgtcaa cgatgagtat gagaggcata cgatcatctc ccgatatgcg tctcgatggc
    11281 gctcaggagc tcatccttgg tgaagggttt ggtcagatag tgttccgagc cgacgatccg
    11341 gccgcgcgcc cgatcgaaca gaccgtcctt cgaggacagc atgatcaccg ggatggagcg
    11401 aaaaaccttg ttgtgcttga tcagcgagca ggtctgatac ccatcgagcc gcggcatcat
    11461 gatgtcgacg aagacgatgt cgggctggtg gtcggcgatc ttggcgagcg catcgaaacc
    11521 gtcgatggcg gtgaagacct cgcagccttc ctttgccagc agcgtctcgg cggtccggcg
    11581 gatcgtcttg ctgtcatcga tcaccagcac cttcaggccc tggagtttgg tgttgttgcc
    11641 ggtcacgttg agggcaactc ctctcatgcg gttggcgcaa attgcgggga cgggcgcttt
    11701 ccgctgctcg cgcggacgtc ctcgttctgc ggatcaccca tggtgacggc ctcaggctcg
    11761 ccaccccagc gaatcgatct tgccatgacg aatcggtcac acaggttgtc agggcgctcg
    11821 atctgcggcc ttcgatcgag ccctttcggg aaatcttgcc acggcggggc agtcacgcag
    11881 gttatcaaag acccccgact tgccgattcc tcgcggcgct cgcgcagcgc gggttttaac
    11941 atatcggcac accccctgct atggcatgcg cgtcacgttt ttcgaagatc gggtgctttc
    12001 atccatggcc cgcgcctggc cgcggggcgc acaagaccgt cactgacata agatagggcc
    12061 tcttcagcag gtcgggcgca gccgatgaaa agactcgggg tggtgatgga ccccatcgac
    12121 gccatcaagt atgcgaagga ctccacgctt gccatgctgc ttgctgcgca ggagcgcggt
    12181 ttcgagctcg aatacatgga gcagagcgac ctctcgctcc gtgacggtat cgccttcggg
    12241 cgcgtgcgcg cgctgacggt gcgggctgat gcgcagaact ggttcacgct cggggaaccg
    12301 cgtctggagc ggctgggggc gctcgacgcg ctcctgatgc gcaaggaccc gccattcgat
    12361 accgaatata tttacagcac ttacatactc gagcgggccg aggagcaggg gaccctggtc
    12421 gtcaaccgac cgcagggact gcgggacatg aacgaaaagg tctatacggc atggttcccc
    12481 cagtgctgcg cgcccacgct gatcactcgc gagatgggcg acatggacag cttcctgcgt
    12541 gagcacggca agatcgtctg caagcccctc gacggcatgg gcggccgctc gatcttcgtc
    12601 ctcgagcgca acgacaagaa catgaacgtg gtgttcgaga cactcaccgg gtacggcagc
    12661 cgcttcgcga tcgcccagcg ctacatcccg gacattgtcg acaccggcga ttcgcgagtg
    12721 ttgctgatcg acggcgagcc agtaccgtat gcgctcgcca gaatccccac agccaccgat
    12781 catcgcggca atctcgccgc gggagcgcgc ggcgtgggcc ggccactgaa cgaccgcgac
    12841 cgttggcttg cggcccagat cgggccgacg ctcgctgcca agggaatgct gttcgtcggg
    12901 ctggatgtta tcggcggtta cgtgaccgag atcaacgtga cgagtcccac gggcatccgc
    12961 gagctggacc gccagtttgg aatcgacatc ggcgctgtgc tgatcgaggc gctcgagcga
    13021 cgattgcagg cctccagcgg cgaggtgagc gcaggtgcgt gaactgcgat gacacggcga
    13081 gctccctcgc tgcgcgaagg caaggcacca gtgcgcgatc ggctcgtgac catgctgttt
    13141 ctcgccgcac tgcttcacgg cgtcgtgatt ctcggcctga cgttcgacgc ctcggccggc
    13201 ggcagggact ccgcgcccgg gctgaaggta ctgctcgtct cggatgaggt ccccgaggcc
    13261 gagcgcaacg acacagccac ctacctggcc cagcgcaccc aactgggctc gggcaacacg
    13321 accgagtcag tgtccccgcg caatccagcc tcttccgtgc ccgtgccgca gcaggacggg
    13381 gtccccgagg gaaattccct gaaccgccag gcggactggg ccggccatcc cgatgagagc
    13441 gtgatcacga ccacggcctg gaagacggac gtgcactatc aagccgaaga aggcaacgca
    13501 ggcacctcgc ccgaccagcc gctcctgctg caggcgcaga gctcggctca gcccggacct
    13561 gaggaggaca gtgggcccgc gcaactcagc ggtccaaagc gcgatgagct gtgggtcaca
    13621 ccggatacgc gcgccgccac actggcaccc tatctggaca gctggcgccg caaggtggag
    13681 cgggtgggga cgattaatta tcccagtgcg gctcgaaact ccggcctgca ctcgaacccc
    13741 gtggtcgagg tcggtatctc ctccgacggc aaactcgaaa ggatgctcat ccagcgttcc
    13801 agcggcgatg cggagctcga tgccgccgcg ctggccattc tcaagctcgc cagtccgttc
    13861 gaccccttcc cgcccgagct ggcgagagag catcgcgtgc tgcgattcgt ctacgaatac
    13921 cagttctccg gcggccggct gcagggcgga gcggtgtcga cagttccttg agccggccgc
    13981 gcgccgtcct catactgcac tcatgacgga aaaggaaagc atggggattc tcggtgacga
    14041 gccggcctcc gagggcgagg aggggccgtc cggcccggtt tcgggtctca ccaatcagct
    14101 tctgatcgcc atgccgtcgc tgctggatcc gaacttttct cagacggtgg cgctcatctg
    14161 cgagcacacc gacaagggcg cgctcggcat cgtgctcaac aagcccttac cgatgcggct
    14221 gtcggacgtg ctcacgcaga tgaagctcga gccctcgagc gaggccattg cctccctgcc
    14281 cgtgttgcgc ggcggccccg ttcagaccga ccggggcttc gtgctgcacc ggcccggcgg
    14341 gcaatgggac catacccata aggtctcgga cagcattcag gtgacgactt cgcgcgacat
    14401 cctggctgcg atggcgcgcg gcgagggacc tgtcgatgcg ttcatcgcac tgggctatgc
    14461 cggttgggag tccgggcagc tcgagcgcga gatccgcgag aacgcctggc tgtcgatgcc
    14521 cgtcgatgcc cgtgtcgtgt tcgagctccc cttcgaggaa cgctggctgg gcgcctggcg
    14581 actcatgggc atcgatgtcg atcggctgag cctggttgcg ggccatgctt gagcaggtga
    14641 tggggcagcc ctccggtacc gccggggcat gaaacggccc gataccgttc tcgcattcga
    14701 cttcggactc aagcgcatcg ggctggctgc cggtgagaca ctcgcgggta gcgccgcccc
    14761 ccgccccgcg ataccggtga tgcgcggcag ccccgactgg agtgccatag agcgcgaggt
    14821 gcgcatgtgg catccgcagg tgctcgtggt tggcgcgccg tataatgtcg atggaactcc
    14881 gggagcgctc gccgaagcag cgcgtgcttt cgccgccgag ctgggatccc gcttcggact
    14941 cccgatcaac atggttgacg agcgttggtc ctcactggaa gcggccaccg ctctcaagat
    15001 gcggcgcgcg agcggcgcgc gccgccggcg gatccggcgc gaggacatcg acagtgccgc
    15061 ggccgcagtg atcctggagc gctggttggc cggggagggg cgtccacatg agtgattcgc
    15121 agatcgagca gcgcaggccg gatggaagcc tgcgccacct gctgacactc gagaacctgc
    15181 cccggcgcga gatcgagcgg ctgctcaaca aagccgagag cttcgtgcgg ccgctgggag
    15241 cgcgtccgcc cacgagcaac gcgctcgccg gggtcacggt ggccaatctc ttcaccgaac
    15301 cctccacgcg cacccgggtc tcgttcgagc tggctgccaa gcgactcggg gcgcacgtgg
    15361 tgaatctcga ggtacagctc tcatcccgcg tcaaaggcga gagcatgctc gatacgctct
    15421 acacccttca gtccctgcac gtggacgtca tggtgatccg cgatgccgag ccgggagtgc
    15481 cggcgctcgc ggccgctaat gtggcacccc acgtcagcgt gctctccgcg ggcgaagcgc
    15541 atgttgccca tcctactcag ggattgctgg atgcgctcac cgtccgacag cacaagaaac
    15601 gcttcgaagg cctgagtatc gccattgtcg gcgatatccg gcattcgcgt gtcgcgcgct
    15661 cggcccatca tgcattcagt gctctcggcg tcagcgacct gcgcatcatc gcaccggcag
    15721 cgctcatgcc ggaagccaat gaattttccg gctgcacgcg gcacacgaca ctggataccg
    15781 gcctgctgga tgctgacgtg atcat
//
LOCUS       DASRHN010000064        10164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_688134, whole genome shotgun sequence.
ACCESSION   DASRHN010000064 DASRHN010000000
VERSION     DASRHN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688134"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFB37_02480"
     CDS             complement(<1..532)
                     /locus_tag="VFB37_02480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZO21342.1"
                     /translation="MAEPTFTIRASWALHAAAAVGLVMRPSLWPWMLGTVVADHLVLT
                     AAGLWPRSSLLGPNWTRLPKAPSTADAVAITIDDGPDPEITPEVLDVLARHDAKATFF
                     CIGERIAQNAPLAREIVHRGHTIENHSQRHLHSFSVLGPRGMREEIERAQNTIAAVTG
                     EQSRFFRAPAGLRNPFL"
     gene            complement(525..1226)
                     /locus_tag="VFB37_02485"
     CDS             complement(525..1226)
                     /locus_tag="VFB37_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21343.1"
                     /translation="MRTIPDLLEPHGVAPTRVLFLPGAYTRPEDFLQAGFAEAVRARQ
                     LPIDLVFAEPGLQHLADRTLLRRLRHELVLPARALGCHSIWICGISLGGFIAMAYAER
                     YANELDGLCLLAPYLGNHIITGEIARAEGVAAWQPGELAADDDERRIWRFIKTHESRP
                     LPLHLGFGSEDRFADSQRLMAAVVRPEHLDVIPGGHEWPVWRQLWENFLDRQFAASRE
                     SLPGIASPSARGGHG"
     gene            complement(1229..3583)
                     /locus_tag="VFB37_02490"
     CDS             complement(1229..3583)
                     /locus_tag="VFB37_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO21344.1"
                     /translation="MIVWVGLLGLACIVVVRAHYTADLSAFLPRAPTATQRLLVEQLR
                     EGVASRLILIGIEGGDAAARARASLELGRRLRADGQFVSVQNGEPVGLEQDRALLFKH
                     RYLLSEAVTPEHFSVAGLHEALQSSLDALASPAGMLLKSLLPEDPTGEMLQIIDQLGN
                     EQQPPMVDGAWSSRDGKRALLVAQTRAAGSDTDGQERAVKTIRAQFQAAAPAANAAGS
                     SSSTHLLLEMSGPGVFSVEARGTIKHEVMRLSILSTVLIMLLLLQIYRSFAALVLGFI
                     PVASGALAGIAAVALGFGLVHGITLGFGVTLIGESIDYSIYLFIQARQPAGSDWKRML
                     WPTIRLGMLTSVVGFASLLPSAFPGLAQLGLFSISGLVAAALVTRFVLPNLIPAGFDI
                     RDTARIGVGFAALLRRVRMPRALLIIVPVLAGIVLYTHRASLWNRELSALSPVSQADQ
                     QLDARLRADVGAPDPRYLIVVSGADQEAVLQAAEKVGQRLDPLVASGILTGYESPARY
                     LPSAALQRARQASLPDPAQLRERLQAALSGLPIRLERLEPFLHDVEAARHQPLLRRAD
                     LEGTSLATGVDALLLRDGSRWSALLPLRVPASFAMAASPVHSAVSEAAPGEAVVLDVK
                     AQADQLYSTYLSEVLKLSLAGFVGIIALLLIVLRSPMRVLRVVAPLVLAVLSVAAILV
                     LLGRELTILHVIGMLLIVAVGSNYALFFDRRANDRHAGEVPLTLASLVIANCATVVGF
                     GVLAFSVVPVLSALGSTVAPGALLALVFSALLAGDLPAPDRAAA"
     gene            complement(3604..4197)
                     /locus_tag="VFB37_02495"
     CDS             complement(3604..4197)
                     /locus_tag="VFB37_02495"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HZO21345.1"
                     /translation="MRRSRRVLACTLLLSAAIASAQTRPQGKSPDLDRLMSALAQRPH
                     GHVTFLERKYLAILDRPVESSGELLYDAPDRLEKRTLKPKPESLVLQGGVVRAQRGRH
                     TYTLDLQRYPQVVPFIESIRATLAGDEAALERVFKVRFEGSFDHWKLLLVPLDPKLAA
                     KVREIHIQGEASAIHSVEIQEADGDRSVMTIGADVGR"
     gene            complement(4184..5116)
                     /locus_tag="VFB37_02500"
     CDS             complement(4184..5116)
                     /locus_tag="VFB37_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21346.1"
                     /translation="MAIKADELHARAEWARTRERGSVTLLRVMSFISLHLGRQVGRVV
                     LYLIAAYFFAFAPAARRQSRVYLRRVLGREPTAADRFRQVLSFASTIHDRVYLLNDRF
                     DLFDVECEGEHWVQQALHAGQGAFLMGSHLGSFEIIRSVGLRQPGLHVAMAMYEGNAR
                     KVNEQLTAINPAARGHIIPLGHMDAMLRIQEALDRGTFMGILADRTLGHEPSLTVPFL
                     GKPAAFPVGPMRAAALMRRPVIFMAGLYRGGNRYHVVFEPLADFSSVSRKERDAAIHA
                     AVVRYAAVLEKYCRTDPYSWFNFYDFWQSADAPQ"
     gene            complement(5100..5432)
                     /locus_tag="VFB37_02505"
     CDS             complement(5100..5432)
                     /locus_tag="VFB37_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21347.1"
                     /translation="MAGLTGSPTRTDLKIAADHPAYAGHFPTHPVLPGVLLLDALLHE
                     LDGPAQRWRIESAKFPSAVQPGEPLSLERETLQSGSIRFVIRAAERIVAQGIVAPSAD
                     GAIRGDQG"
     gene            complement(5437..6870)
                     /locus_tag="VFB37_02510"
     CDS             complement(5437..6870)
                     /locus_tag="VFB37_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO21348.1"
                     /translation="MSRSSARWSTSWTTSARTGRSSVLAKFPLLTHPGPDSIVAYRAG
                     EPVTAARFLADVRRLAAVLPAPAHLLNVCADRYRFAVGFAASLLAGKVSLLPSTHTPE
                     VIKQLRLFAADVFCLTDDPRCPIDLPRVIYPQGAPAPDTEAQSWPPPALDADQVAAYV
                     FTSGSTGSPLPYRKTWGRLVNCVRFEAQCLGLNGQAHTLVGTVPPQHMYGFESTVLMP
                     LQGGHALGSGRPFYPVDICSQLEAVPAPRMLISTPVHLRALLATDTRLPPMALIVSAT
                     APLSLDLAHEVERRFGTRLLEIYGSTETGQIASRRTTATLEWQLFRGVRLVSKAEQTW
                     AEGGHVEQPTLMCDVLEITAEERFLLHGRLSDLVNIAGKRSSLAYLNHQLNAIPGVKD
                     GVFVLREEEAAAAHGVSRVAALVVAPGMSSAALIRELRKRIDAVFLPRPLVLLERLPR
                     NETGKLPRESVHSLLTTALKRSRSASR"
     gene            complement(6806..7111)
                     /locus_tag="VFB37_02515"
     CDS             complement(6806..7111)
                     /locus_tag="VFB37_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZO21349.1"
                     /translation="MSDNASPLRQPARPADNTELQREVAGLVVAALNLDVAAADIDPK
                     APLYGEGLGLDSIDILEIALVVSKQYGVQLRADSEQNEQIFRSLEHLVDYISAHRTK"
     gene            complement(7187..7954)
                     /locus_tag="VFB37_02520"
     CDS             complement(7187..7954)
                     /locus_tag="VFB37_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO21350.1"
                     /translation="MTTRSATHLVLIPSYNPGRKVFETVRGAREQWNPVWIVVDGSTD
                     GTAETLQVMAQTDPGLRVLVRRHNGGKGAAVLDGLVEALRHGYTHVLTMDSDGQHPAE
                     LISTFMAESLAHPRAMVLGEPVFDASAPSVRVKGRRVSNYWANVETLWAGIHDSLFGF
                     RVYPAEPLVRIMKSHMWMRRFDFDVEAVVRLSWSGIEAINLPAPVRYFRPDEGGVSHF
                     RYGRDNMLLTCMHGRLFWGFLVRLPWLLARRVLRIKH"
     gene            complement(7951..8769)
                     /locus_tag="VFB37_02525"
     CDS             complement(7951..8769)
                     /locus_tag="VFB37_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZO21351.1"
                     /translation="MSSPERAAGSRPLKIFADYLVTYAMVIMLGLICLVFSLWALIAY
                     PLLPRRWGIAAGRAGIMAGFRLYSWLLTFTGAYRLDLTGIDSLRGGPPVILAPNHPSL
                     IDALLILSRHPDLACVMKAALMRNLFLGAGSRLARYIPSDHPRQMIREAVADLRRGGV
                     LLLFPEGTRTTHDPVNTLKASVAVIAKHARVPVQVAIIETDSPYAGKGWGMFRRPVLP
                     ITYRIRLGRRFDPPEDVTAFMSALEREYAQQLTGALQSDWLGHERGSQADSYGR"
     gene            complement(8766..9599)
                     /locus_tag="VFB37_02530"
     CDS             complement(8766..9599)
                     /locus_tag="VFB37_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HZO21352.1"
                     /translation="MTGTRRRTLTLAALAASLAIAAHADQKPLWEFGLGVGTVVFEDY
                     RGADTTHAYVLPLPYLYYRGKFLQSDRNGIRGRLFDQDWIELNISLNATTPVRRNAAR
                     AGMPDLRPTFEIGPSLDMHLWKSASQKVKFDVRIPLRKAFTFEAPPRAIGWVFAPNAS
                     VDVADLAGLQGWYFGALAGPLFADRRYHDYFYTVAPQYATAERPAFQASGGYAGTQVL
                     ASLSKRYPAYWVGAYVRHDSLAGAVFESSPLVKRNSYWAGGLGIAWIIHQSAGTVEAA
                     E"
     gene            complement(9608..>10164)
                     /locus_tag="VFB37_02535"
     CDS             complement(9608..>10164)
                     /locus_tag="VFB37_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZO21353.1"
                     /translation="ALAACGRDLTGNVPAACALGSLSGSPLVIYFLASHEAPTAIENP
                     RQVSAYEYPPQYGPKPAFSRASVLRERSGAMLFVSGTSSIVGHRTVHAGDVAAQTRET
                     LTNIEALVEEANRVTGASRFAIGTLAYKVYVRKVADLPTIQSQLHAALGRQAQVMYLH
                     AEICRQDLLVEIEAIGRAPESAAA"
BASE COUNT         1893 a         3290 c         3443 g         1538 t
ORIGIN      
        1 ccagaaacgg attccgcagt ccggcgggcg cgcgaaagaa gcgcgactgc tcgccggtaa
       61 ccgcggcaat ggtgttctgg gcgcgctcga tttcctcccg catgccgcgc gggccgagca
      121 cggagaagct gtgcagatgg cgctggctgt gattctcgat cgtatgaccg cggtgcacga
      181 tctcgcgtgc cagcggcgcg ttctgggcaa tgcgctcgcc gatacagaag aaagtcgcct
      241 tcgcgtcatg tctcgccaac acatccagta cctcgggcgt gatctccgga tccggcccgt
      301 cgtcgatggt gatcgcaacc gcgtcagcgg ttgagggggc cttcggcagc cgcgtccagt
      361 tggggccgag gagcgagctt ctcggccaga gccccgcagc cgtgagcacc aggtgatcgg
      421 caaccaccgt gcccagcatc cacggccata ggctgggacg catgacgagg ccgactgccg
      481 ccgcggcgtg cagcgcccag gaggcccgga tggtgaatgt cggctcagcc atgtccgccc
      541 cgcgcactgg gactggcgat gcccggcagg gactcacgcg aggcggcgaa ttgccggtcc
      601 aggaagttct cccacagttg ccgccagacc ggccactcgt gccctcccgg gatgacatcg
      661 aggtgctccg gtctgaccac ggccgccatc aggcgctggc tgtcggcaaa gcgatcctcg
      721 ctgccaaatc ccaggtgcag cggcaacggg cgcgactcat gagtcttgat gaagcgccag
      781 atgcgccgct cgtcatcatc cgcggcgagc tcgccgggct gccacgcggc tacaccctcc
      841 gcgcgcgcga tttcccccgt gatgatgtga ttgccgaggt agggcgcgag caggcacagc
      901 ccatcgagct cgttcgcata ccgctccgca taggccatgg cgatgaatcc gccaagcgag
      961 atgccgcaga tccagatcga gtggcagccg agggcccgcg cagggagaac caactcatgg
     1021 cgcaggcggc gcagcagcgt ccgatctgcg aggtgctgca gtcccggctc cgcgaatacg
     1081 aggtcgatcg gcagttgccg ggcgcgcacc gcctccgcaa accccgcctg cagaaagtcc
     1141 tcgggacgtg tgtaggcgcc cgggagaaac agcacgcgtg tgggggcgac gccgtgcggt
     1201 tcgaggagat cggggatcgt gcgcatgctc aggccgccgc tcgatcgggg gcgggcaggt
     1261 cgccagccag gagagcggag aaaacgagtg ccagcaacgc gcccggggcc actgtggatc
     1321 ccagagcgga cagcaccggg accacggaga aggcgagcac gccgaagccc accacggtgg
     1381 cgcagttggc gatgacgagt gaagccagtg tcagcggaac ctcgccggcg tgtcgatcat
     1441 tcgcgcggcg atcgaagaaa agcgcgtaat tcgatccgac cgccacgatg agcagcatcc
     1501 cgatcacatg caaaatcgtg agttcgcgtc cgagcaggac cagaatggca gcgacgctca
     1561 gcacggccag cacgagcggg gccacgacgc gcaacacacg catcggcgag cgcagcacga
     1621 tcagcagcag cgcaatgatg cccacgaatc ccgctaacga aagtttgagg acctctgaca
     1681 ggtaggtcga gtagagctga tccgcctgcg ccttcacatc gagtaccacg gcctccccgg
     1741 gggcggcttc ggaaaccgca gaatgcacag ggcttgcagc catggcaaat gaggccggca
     1801 cacgtagcgg cagcagcgca ctccatcgac ttccgtcccg aagcaggagc gcatcgacgc
     1861 cggtcgcgag cgaggtcccc tcgaggtccg ctcgccgcag cagaggctgg tggcgagcgg
     1921 cctcaacatc gtgcagaaac ggctccagcc gctccaggcg gatcggcagg ccgctgagcg
     1981 ccgcctgcag gcgctcgcga agctgcgcgg ggtccggcag gcttgcctgc cgcgcccgtt
     2041 gcagcgcggc gctcggcaga tagcgggccg ggctttcgta gccggtgagg attcccgatg
     2101 ccaccagggg atccaggcgc tggccgacct tttcggcggc ctgaaggacc gcctcctggt
     2161 cagctcccga gaccacgatg aggtagcggg gatccggcgc accgacgtcg gcccgcagcc
     2221 gggcatcgag ttgctggtcg gcctgcgata caggactcaa cgccgagagc tcgcggttcc
     2281 acagcgaggc ccgatgggtg tagaggacga tgccggcaag caccggcacg atgatcaaca
     2341 gtgcgcgcgg catgcgaacg cgcctcagga gcgccgcgaa accaactcca atgcgggccg
     2401 tgtcgcggat gtcgaatccg gcgggaatca ggttcggcag cacgaagcgg gtcacgaggg
     2461 cggccgccac gaggccgctg atggagaaca gcccgagctg ggccagaccc ggaaatgccg
     2521 agggcaggag cgatgcgaat ccgacgacgg aagtcagcat gcccagccgg atcgtcggcc
     2581 agagcatgcg cttccagtcg ctgccggcgg gctgccgcgc ctggatgaag agatagatcg
     2641 agtaatcgat ggactcaccg atcagcgtca cgccgaaccc gagagtgatg ccatgcacca
     2701 ggccgaagcc gagcgccacg gccgcaatac cggcgagcgc gccggaggcc acgggaatga
     2761 accccagcac cagggcggcg aaggagcggt agatctgcag cagcagcaac atgatcaaca
     2821 cggtgctgag aatcgacagg cgcatcacct cgtgcttgat ggtgccgcgg gcttccacgg
     2881 aaaacacacc gggtccgctc atctcgagca gcaggtgcgt gctgctcgag ctgcctgccg
     2941 catttgcagc gggcgcggca gcctggaact gtgcccgaat ggtcttcacg gcacgctcct
     3001 gcccgtccgt gtcagatcct gcggcgcgtg tctgggccac gagcagcgcg cgtttgccgt
     3061 cgcgcgagct ccaggctcca tcgaccatgg gcggttgctg ctcgtttccc agctgatcga
     3121 tgatctgcag catctcgcct gttggatcct ctggcagcag ggatttcagg agcatgccgg
     3181 caggggaggc gagcgcatcg agcgagctct gcagggcctc gtgcaggccc gccacgctga
     3241 aatgctcggg agtgactgcc tcactcagca ggtagcgatg cttgaagagc aatgcgcgat
     3301 cctgctcgag acccaccggc tcgccgttct ggaccgacac gaattgcccg tcggcgcgca
     3361 gtcgccggcc cagctcgaga gaagcgcgtg cgcgagcagc cgcatcgcct ccctcgatgc
     3421 cgatcagaat caaacgcgac gcgacccctt cgcgcagctg ctccacgagc agtcgctgag
     3481 tcgccgtggg cgctcgcggc agaaaggccg agagatcggc cgtgtagtgg gcgcggacaa
     3541 ccacgatgca ggccagaccc agcaggccga cccaaacaat catcgccggc aaacccgccc
     3601 ggctcatcgc cccacgtctg ccccgatcgt catcaccgac cggtcgccat cggcctcctg
     3661 gatctcgacc gaatggatgg cagaggcctc accctggata tgaatctcgc gcaccttcgc
     3721 ggcgagcttc ggatcgagcg gcacgaggag cagcttccag tgatcgaagc tgccctcgaa
     3781 gcgcaccttg aagacacgtt cgagtgcggc ctcatctcct gcgagtgtgg cccggatgct
     3841 ctcgatgaac gggacgactt gcggatagcg ttgcaaatcg agcgtgtagg tatggcggcc
     3901 gcgctgggct cgcacgacgc cgccttgcag cacgaggctc tcgggtttgg gcttgagggt
     3961 gcgtttctcg agccggtcgg gtgcatcgta aagcagctcg ccggaggact cgacgggccg
     4021 atcgagaatg gcgaggtact tgcgctcgag gaacgtgaca tgcccgtgtg ggcgctgggc
     4081 cagcgcgctc atcagccgat cgaggtcggg agatttaccc tgcgggcggg tttgcgcaga
     4141 ggcgatggcc gcgctcagca gcagcgtgca ggccaggact cgcctactgc ggcgcatcgg
     4201 cgctctgcca gaagtcataa aagttgaacc agctgtacgg atcggtgcgg caatacttct
     4261 ccagcacagc cgcgtaacgc acgaccgctg cgtggatcgc cgcatcgcgc tccttgcgac
     4321 tgacgctgga aaagtccgcc agcggctcaa agaccacgtg atagcgattg ccgccacggt
     4381 ataagccggc catgaagatg accggccgcc gcatgagcgc ggcggcccgc attgggccca
     4441 ctggaaaggc ggccggcttg cccagaaacg gcaccgtgag gctcggctcg tgacccaagg
     4501 tgcgatcggc cagaatgccc atgaaagtgc cgcgatcgag cgcctcctgg atgcgcagca
     4561 tggcatccat atgtccgagc ggaatgatgt gcccccgggc cgccggattg atggcggtga
     4621 gctgttcatt gaccttgcgg gcgttgccct cgtacatggc catcgccacg tgcagtccgg
     4681 gctggcgcaa cccgacggag cggatgatct cgaagctccc gaggtgcgag cccatgagga
     4741 aggcaccctg accggcgtgc agcgcctgct gcacccagtg ctctccctcg cactcgacat
     4801 cgaacagatc gaaacgatcg ttcagcagat agacgcgatc gtggatcgtg ctggcgaaac
     4861 tcaacacctg gcggaagcgg tccgccgcgg tgggttcgcg gcccaatacc cggcgcaggt
     4921 agacccggct ctggcggcgc gcggcgggcg caaaagcgaa gaagtacgcc gcgatgagat
     4981 agaggaccac gcgaccgacc tgacggccca gatgcagcga gatgaagctc atcacgcgca
     5041 gcagcgtgac gctgccgcgc tcacgggtcc tggcccattc ggcgcgcgcg tgcagctcgt
     5101 cagccttgat cgccacggat cgctccatcc gctgaggggg ccacgatacc ctgggcgacg
     5161 atgcgctcgg ccgcccggat cacgaagcgg atcgagccac tctgcagggt ttcccgctcg
     5221 aggctgagcg gctcgccggg ctgcacggcg ctggggaact ttgcagactc gatgcgccag
     5281 cgctgggcgg ggccatccag ctcgtgcaac agcgcatcca gcagcaacac acccggcagc
     5341 accgggtgag tggggaaatg cccggcgtag gccggatgat ccgccgcgat cttcagatcg
     5401 gtgcgtgtcg gggagcccgt gagcccggcc atgctcttac ctggaggccg agcgcgagcg
     5461 cttcagagcg gtggtcagca gcgagtgcac gctctcgcgc ggcagcttgc cggtctcgtt
     5521 tcgcggcagc cgctcgagca gcacgagcgg tcgaggcagg aatacggcat cgatgcgctt
     5581 gcgcaattcc cggatcagcg cagccgagct catacccggg gcgacaacca gagcggccac
     5641 gcggctgacg ccgtgcgctg cggctgcctc ttcctcgcgc aacacgaaca caccgtcctt
     5701 cacgcccggg atcgcattca actgatggtt gaggtaggcg agcgagctgc gcttgccggc
     5761 aatgttgacc agatccgaca gccggccgtg cagcagaaag cgctcctcgg cagtgatctc
     5821 cagcacatcg cacatcagcg tcggctgctc gacatggcct ccttcagccc acgtctgctc
     5881 ggccttgctc accagccgca cgccgcgaaa cagctgccac tcgagggtcg cggtcgtgcg
     5941 ccggctcgcg atctggccgg tctccgtcga gccgtaaatc tccagcaggc gcgttccgaa
     6001 acgccgctcg acctcgtgcg ccagatccag cgacaggggc gcggtggccg acacgatgag
     6061 agccatcggc ggcaggcgtg tgtcggtggc aaggagtgcc cgaaggtgga ccggggttga
     6121 gatcagcatc cggggtgctg gcacggcttc gagctggctg cagatatcga ccggatagaa
     6181 gggccgtccg ctgcccagag catggcctcc ttggaggggc atcagcacgg tggactcaaa
     6241 gccgtacatg tgttgtggag gcaccgttcc cacgagggta tgcgcttggc cgttgaggcc
     6301 cagacactgc gcctcgaagc ggacgcagtt gacgagtcgt ccccaggtct tgcggtaggg
     6361 cagcgggctg ccggtggacc cggaagtaaa tacataggcc gcgacctgat ctgcatcgag
     6421 ggccgggggc ggccagctct gcgcctcggt gtccggcgcc ggcgcacctt gcggatagat
     6481 cacgcgcggc aagtcgatcg ggcacctggg gtcatcggtg aggcagaaca catcggcagc
     6541 gaacagcctg agctgcttga tgacttccgg ggtgtgggtc gagggcagca ggctgacctt
     6601 gcccgccagc aggctggccg caaagccgac cgcgaagcga tagcgatcgg cgcagacatt
     6661 gagcagatgg gccggtgcgg gcagaaccgc ggcgaggcgc cgcacatcag cgagaaagcg
     6721 ggcggccgtg acgggctcgc ctgcccgata ggcgacgatc gagtcggggc cagggtgggt
     6781 cagcagcggg aacttggcca ggacgctact tcgtccggtg cgcgctgatg tagtccacga
     6841 ggtgctccag cgagcggaag atctgctcat tttgttcgct gtcggcacgg agctgcacac
     6901 cgtactgctt ggacacgacc agggcgatct cgagaatgtc gatcgagtcc agaccgaggc
     6961 cttcgccgta gagaggggcc ttgggatcga tgtcggcggc ggcgacgtcc aggttcagtg
     7021 ccgccacgac cagaccggcc acttcccgtt gcagctccgt attgtcagcc ggccgcgccg
     7081 gttgtcgaag aggcgacgcg ttgtcggaca tatcctgaac gattcaatcg ggagctgtta
     7141 tctggccatg atacatgggg ttagctggag cgcaaccaga gtggcttcag tgcttgatcc
     7201 gtagcacacg gcgggccagc aaccacggca gacgcaccag gaagccccaa aaaagtcggc
     7261 cgtgcatgca ggtgagcagc atgttgtcgc ggccataacg aaaatgcgac acgccgccct
     7321 catccgggcg gaagtaacgc accggcgcgg gaaggttgat tgcctcgatg ccgctccagc
     7381 tcagccggac gactgcctcg acatcgaaat cgaaccggcg catccacatg tgcgacttca
     7441 tgatgcgcac cagcggctcg gccggataga cgcgaaagcc gaagagcgag tcgtggatcc
     7501 ccgcccagag cgtctcgaca ttggcccagt agttggaaac gcgacgaccc ttgacgcgca
     7561 cgctgggggc gcttgcatcg aaaacgggct ctccgagaac catagcgcgc ggatgcgcga
     7621 gcgactccgc catgaaggtg ctgatcagct cggctggatg ctgtccgtcc gaatccatcg
     7681 tcagcacgtg cgtgtagccg tgccgcaggg cttccaccag cccgtccagc accgctgcgc
     7741 ccttgcctcc attgtgacgg cgcaccagga cacgcaagcc agggtcggtt tgcgccatga
     7801 cctgcaaggt ctcggcggtc ccgtcggtgc tgccatccac gacgatccag accggattcc
     7861 actgctcgcg cgcgccccgg accgtctcga aaaccttgcg ccccgggttg tagctcggaa
     7921 tgagcaccag atgagtcgcc gacctcgtgg tcatcgaccg tagctgtcag cttgcgatcc
     7981 ccgctcgtgg ccgagccaat ccgattgcag ggcgcccgtg agctgctgcg cgtattcgcg
     8041 ctcgagcgcc gacatgaatg ccgtgacgtc ctccggcgga tcgaagcgcc ggccgagccg
     8101 gatccgatag gtgatcggca gcacgggccg gcggaacatg ccccagccct tgcccgcgta
     8161 gggcgaatca gtttcgatga tggcgacctg cacgggcacc cgcgcgtgct tcgcgatcac
     8221 cgcgacgctc gccttgagag tgttgacagg atcgtgcgtg gtccgggtgc cttcgggaaa
     8281 cagcaacagc acgccgcccc gacgcagatc ggccacggcc tcccggatca tctgtcgggg
     8341 atgatcgctc ggaatgtagc gcgccaggcg cgaacctgcg cccaggaaca gattgcgcat
     8401 cagcgcggct ttcatcacgc aggcgagatc cgggtggcgc gacagaatga gcagcgcatc
     8461 gatcagggag ggatgattgg gcgcgaggat caccggcgga ccgccgcgca gcgaatcgat
     8521 cccggtcagg tccagccggt aggcgccagt gaaggtgagc agccaggagt acaggcgaaa
     8581 gccggccatg atcccggcgc ggccggcggc gattccccag cggcgcggca gcagcggata
     8641 ggcaatcagc gcccagaggg agaacaccag gcagatcaga cccaacatga tgaccatcgc
     8701 gtaggtcacc agatagtcag caaaaatctt cagcggccgc gacccggccg cgcgctccgg
     8761 cgagctcact ctgccgcttc gaccgtaccc gctgactgat ggatgatcca cgcgatcccg
     8821 aggcctccgg cccaatagct gttgcgcttg acgagcgggc tcgactcgaa tacggcgcct
     8881 gccagcgaat catggcgcac gtaggccccc acccagtatg caggatagcg tttcgatagc
     8941 gaggcgagaa cctgggtccc ggcgtacccg ccgctcgcct ggaaggccgg gcgctcggcc
     9001 gtcgcatact ggggggcgac cgtgtagaaa taatcgtgat agcggcgatc cgcgaacaag
     9061 ggacccgcca gcgcgccgaa gtaccagccc tgcaagccgg ctaaatcggc gacgtccacg
     9121 ctggcattgg gcgcaaagac ccacccgatc gcgcgcggcg gagcttcgaa ggtgaaagcc
     9181 ttgcgcaagg ggatccgcac atcgaatttc accttctggc tcgccgactt ccacaggtgc
     9241 atgtccaggg aagggccgat ctcgaaggtt ggacgcaggt ccggcatgcc ggcgcgcgca
     9301 gcgttgcgcc gcacgggggt cgtcgcattg aggctgatgt tcagctcgat ccagtcctga
     9361 tcgaagagcc gcccgcgtat cccgttgcga tcggattgca gaaactttcc gcggtaatag
     9421 aggtagggca ggggcaacac ataggcgtgc gtggtgtcgg caccccgata gtcctcgaag
     9481 accaccgtgc cgacgccgag cccgaattcc cacaggggct tctggtccgc atgggccgcg
     9541 atcgcaaggg aagctgccag cgccgcgaga gtgagggtcc ggcggcgggt gcccgtcatg
     9601 agcactctca ggccgcagcg ctctcgggcg ctctgccgat cgcctcgatc tcgaccagca
     9661 gatcctggcg gcagatctcg gcatgcaggt acatcacctg cgcttgccgg ccgagcgcgg
     9721 cgtggagctg ggattggatc gtggggaggt ccgcgacttt gcgcacatag actttgtacg
     9781 ccagggtgcc gatggcgaat cgggatgctc ccgtcaccct gttggcttcc tcgaccaggg
     9841 cttcgatgtt cgtgagggtc tcgcgggttt gcgcggcgac atcgccggca tgcacggtcc
     9901 gatgcccgac gatgctcgag gtcccggaga cgaacaacat cgcgccggag cgctcacgca
     9961 gcaccgaggc gcgcgagaac gccggcttcg ggccgtactg cgggggatat tcgtacgcgc
    10021 tcacctggcg cggattctcg atggcggtgg gtgcctcgtg actggcgaga aagtagatca
    10081 ccagtgggct gccgctcaac gagcccagcg cgcaggccgc gggcacgttg ccggtgagat
    10141 cacggccgca ggcggcgagc gcct
//
LOCUS       DASRHN010000065         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1342058, whole genome shotgun sequence.
ACCESSION   DASRHN010000065 DASRHN010000000
VERSION     DASRHN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342058"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VFB37_02540"
     CDS             complement(<1..1084)
                     /locus_tag="VFB37_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HZO21354.1"
                     /translation="MPAPASSVETRTARPQIGAWGLDLTTQDSSVKPGDDFYRYAEGK
                     WMDANQIPPDRTSWTSAAELADRAEHNIRAIVEALPREAPPDSIEQKVGDYYRAYLDA
                     DTIEHLGLAPARPGLEAIAAARTHEQLATLLGRPDLDLKSPLIIAITIDQKNPDRYIA
                     VVTQSGLSLPDRDYYLKDEPVFTELRAKYVAHVERMLTLAGEAHAGAKAAWVLDIETQ
                     IAHLHWPAAKRRERDLTYNLRTRRELEQLEPGFPWTQVLTAQGLQSQSEFVVRELDAV
                     QGLARLFRHIPVERWRAYLEYQYLAGAADVLPAAFDAERFDFYGHTLNGQPRQRDRWK
                     RAVAAVNLGLGEAIGQLYVQQYFPAAA"
     gene            complement(1180..1548)
                     /locus_tag="VFB37_02545"
     CDS             complement(1180..1548)
                     /locus_tag="VFB37_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZO21355.1"
                     /translation="MDARKTLAVAGILIALATALGAFGAHALRAHLTADRLQVYETAV
                     RYHFLNSLGLLAIGVLLRSSDGELLRWSAVLVLAGVILFSGSLYLLTFGAPRMLGIVT
                     PLGGLALIAGWILFAVAVSR"
     gene            complement(1569..2315)
                     /locus_tag="VFB37_02550"
     CDS             complement(1569..2315)
                     /locus_tag="VFB37_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZO21356.1"
                     /translation="MPYGIVVIILGLVLLLSILRVLNEYERGVMFTLGRFTGIKGPGV
                     VFVFPVLQQMRKVDLRVIVLNVPKQDVITRDNVSVKVNAVVYFRVVDPGKAIIQVANP
                     YEATSQVAQTTLRSVLGQHEMDDLLAQREKLNADIQQILDQNTEAWGIKVSNVEIKDV
                     DLDESMIRAMAKQAEAERTRRAKIIHAEGESQAAQMLVQAAESLARQPSAMQLRYLQT
                     LADISHEKTQVIVFPLPLDLIEPLLDRRRE"
     gene            complement(2349..>2621)
                     /locus_tag="VFB37_02555"
     CDS             complement(2349..>2621)
                     /locus_tag="VFB37_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZO21357.1"
                     /translation="LPIIAGIATVGGLVIVAMAALASRSLRRPVVTGVQAMIGDRAEV
                     VQALNGKWRIRYGGELWNAHSATTLQVGQIVRITRVDGLTLWVEPV"
BASE COUNT          501 a          841 c          799 g          480 t
ORIGIN      
        1 tggccgcagc ggggaagtac tgctgcacgt acaactggcc gatggcctcg ccgagcccga
       61 gattcacggc cgcgaccgcg cgcttccatc gatcgcgctg ccgcggttga ccattcaggg
      121 tgtgtccgta gaaatcaaac cgctcggcat cgaaggcggc gggcagcaca tccgcggctc
      181 ccgcgaggta ttgatactcg aggtaggccc gccagcgctc gaccgggatg tgccggaaca
      241 gcctggcaag gccctgcacg gcatcgagct cgcgaacgac gaactcgctc tggctctgca
      301 gtccctgcgc ggtgagcacc tgagtccacg gaaagccagg ctccaactgc tccagctcac
      361 gacgggtacg caggttgtag gtcagatccc gctcgcgccg cttggccgcc ggccagtgaa
      421 gatgcgcaat ctgtgtctcg atatcgagta cccaggccgc tttcgcgccg gcatgggcct
      481 caccggcgag cgtcaacatc cgctcgacat gcgccacgta tttggcgcgc agctcggtga
      541 ataccggctc gtccttcaga taatagtcac gatcgggcag actcaggccg ctttgagtga
      601 ccactgcaat atatcgatcc ggattcttct gatcgatcgt gatcgcaatg atcagcggtg
      661 acttgagatc caggtcaggc cggcccaaga gcgtggcgag ttgctcgtgc gtgcgggcag
      721 cggcgatcgc ctcgagcccg ggccgggcag gggcgaggcc caggtgctcg atcgtgtccg
      781 cgtcgagata cgcacgataa tagtcgccca ccttctgctc gatgctgtcc gggggcgctt
      841 cccgtggcag cgcttcgacg atcgcgcgga tgttgtgctc ggcacgatcc gccagctcgg
      901 cggcggatgt ccaactcgtg cggtctggag ggatctggtt ggcatccatc catttgcctt
      961 cggcgtaacg atagaagtca tcgccgggct tgaccgacga gtcctgagtc gtcagatcga
     1021 ggccccacgc gccgatctgc gggcgggccg tgcgggtttc gacactgctc gccggggctg
     1081 gcatgttggc atgttcagtt ggcatatgag tgctacaggc tgcaagaagc attgccacag
     1141 cagaccgtat ggcgactcga gcgcgcatgt tcaagacctt tacctcgaaa cggcgacagc
     1201 gaacaggatc caacccgcga tcagagccag cccgcccagc ggagtcacga tgcccagcat
     1261 ccgcggcgcg ccgaaggtca gcagatagag gctgccgctg aacagaatga ctcctgccaa
     1321 caccaggacc gccgaccagc gaagcaactc gccgtcgctc gagcgcagca acacaccgat
     1381 tgccagcaat ccaagtgaat tcaggaagtg gtagcgcaca gcggtctcgt agacctgcag
     1441 cctgtcagca gtcaggtgcg ctctcagggc gtgcgcgccg aaggcgccga gggcggtggc
     1501 cagggcgatc agaatacccg caacagccag ggtttttcgt gcatccatgg gcgaagtgcc
     1561 gtccggcgtc attcgcgccg ccggtccagc agcggctcga tcagatcgag cggcagcggg
     1621 aagacgatca cctgcgtctt ctcgtgggag atgtcggcca gggtctgcag atagcgaagc
     1681 tgcatggcac tgggctgacg ggcgagagac tcggcagcct gcacgagcat ctgagctgcc
     1741 tggctctcgc cttccgcgtg aatgatcttc gcccggcgcg tgcgctctgc ttccgcctgt
     1801 ttggccatgg cccggatcat gctctcgtcg agatcgacat ccttgatttc aacattggaa
     1861 accttgattc cccacgcctc ggtgttctgg tccaggatct gttgaatatc ggcgttgagc
     1921 ttctcacgct gagccagcag atcgtccatc tcgtgctggc cgaggactga gcgcagcgtg
     1981 gtctgagcca cctggctggt cgcctcgtaa gggttcgcta cctgaataat ggccttgcca
     2041 ggatcgacga cccggaaata caccacggcg ttgaccttca ccgaaacgtt gtcgcgggtg
     2101 atgacatcct gtttcggcac attcagcacg atgacgcgca gatccacctt gcgcatctgc
     2161 tgcagtacgg ggaatacgaa cacgactccc gggcccttga tgcccgtgaa ccgacccagc
     2221 gtgaacatga caccgcgctc gtattcattc aacacacgca ggatgctcaa cagcagcacc
     2281 agacccagta tgatgacgac tattccatag ggcatgagga tctccgctaa ccttattttc
     2341 gctatcgatc agaccggctc cacccacaag gtgaggccat ccacccgcgt gatccggacg
     2401 atctggccga cctgcagagt cgtggcgctg tgcgcgttcc agagctcccc accgtaacgg
     2461 atcctccatt tgccgttgag ggcctgcacg acctccgcac gatcgccgat catcgcctgc
     2521 acacccgtca ccacaggccg gcgcagagac ctgctggcca gggccgccat ggcaacgatc
     2581 accagtccgc cgaccgtggc gatgcccgcg atgatcggta a
//
LOCUS       DASRHN010000066         4144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_353765, whole genome shotgun sequence.
ACCESSION   DASRHN010000066 DASRHN010000000
VERSION     DASRHN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353765"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2610
                     /locus_tag="VFB37_02560"
     CDS             <1..2610
                     /locus_tag="VFB37_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HZO21358.1"
                     /translation="QGTESPAQTAKLLQALQASNGAATPAALAAAGQPPAAPPGAAPN
                     APNDGNPQDTNMPASPSSQEEPAMSAEDKTRLKDLMTLIRSRNPYQLSHDGALTLPGF
                     EPIPLLGLTEEQASLRLQIEPAFRGVEIRLTRLPLKKTGPLGLKPFGYDLFKEAPSTF
                     APVTNVPVPSDYVIGAGDELSIQLYGSQNRTLKLTVGRDGRIAFPELGPISVAGLVFS
                     KAKADIESRVEHQLIGVRASVAMADTRSIRVFVLGEARRPGSYTISGLGTITSALFAA
                     GGVKRIGSLRRIELKRQGTVVRKLDVYDLLIRGDTTDDAKLLQGDVIFVPPVGPTVSV
                     YGEVRRPAIYEIKNESTVADVVQLAGGLTPQADPVTAMLTRIDANQHRVVLQADILGQ
                     DRSRALVRNGDELRVTRLRPTLDSAVVLQGHVFTAGAFPYHEGMHISDVIHSTDELRP
                     DADLHYVLIRRELPPDRRVAVLSVDLAAAWRSPRSNQDIVLMPRDRITVFDLSSGRDK
                     IIQPLMDELRRQSSAMQPAEVVHVEGKVKVPGEYPLQPGMTVVDLIRAGGGLADAAAY
                     GGTAELTRYRVEKGETRQVQLVNVDLTAALHGDPSQNLKLEPFDDLSVKQVSQWDVQE
                     SVLLRGEVRFPGRYIIRNGDTLRGVIARAGGLTSFAFPEGSVFTRETLKELEQKQMDM
                     LATRMQTALTGLAIRGAAAGVGGGASALTLGQSLLGQLRSAKAVGRLVIDLPRLMREP
                     PGSADDVILRNGDLLIVPKFQQQVTVIGEVQSATSHLYRRGLGRDEYIALSGGLTRDA
                     DKRKIYVVRANGSVVARNGNRWFRGTDVAIKPGDTIVVPLDTERMPPLPFWQAVTGIL
                     YNVALGAAAIRVL"
     gene            2832..3176
                     /locus_tag="VFB37_02565"
     CDS             2832..3176
                     /locus_tag="VFB37_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HZO21359.1"
                     /translation="MSGSLLTEEMRYRLLRLIAANPHMSQRDVARELGISLGKVNYCL
                     RALVQRGWVKAHNFKNSRNKAAYMYLLTPRGIEAKALLAVRFLKIKKHEYERLRQEIE
                     QIRIEAGEPGRR"
     gene            3197..>4144
                     /locus_tag="VFB37_02570"
     CDS             3197..>4144
                     /locus_tag="VFB37_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZO21360.1"
                     /translation="MQTHPAAIRDRPIRIAVVGCGRIAANHFKAIEAHRNELRLVAVC
                     DNDPQALEAAQRRHDVPGYGSIVELLKSEVDVVALCTPSGLHPLHAVLAARAGCHVIT
                     EKPMATRWSDGLSMVRECDEAGVHLLVVKQNRRNATLQLLKSALDAKRFGRVHMVLIN
                     VFWSRPQSYYDSAKWRGTWEFDGGALMNQASHYVDLIDWLIGPVESVQAYIATLARNI
                     QVEDTATVGVRWRSGALGSVNVTMLTYPKNLEGSITILGEKGTARIGGVAVNEIQHWE
                     FEDRNPDDERVRNASYETQSVYGLGHPLYYANVVDVLRGN"
BASE COUNT          760 a         1298 c         1383 g          703 t
ORIGIN      
        1 cagggcacgg aatcccctgc ccagacagcg aagctgctgc aggccctgca ggcctccaac
       61 ggcgcggcaa cgcccgccgc tctggccgcg gccgggcaac ctcctgcggc gccgcctggg
      121 gcggccccca atgcccccaa cgacggcaat ccgcaggaca ccaacatgcc ggcctctccc
      181 agctcgcagg aggagccggc gatgagcgcg gaggacaaga cgcggctgaa ggatttgatg
      241 accctcatcc gctccagaaa tccctaccag ctctcgcatg atggggcgtt gacgttgccc
      301 ggattcgagc cgatcccgct gctagggctc accgaggagc aggcgagcct gcgactgcag
      361 atcgagccgg cgttccgcgg cgtggagatc cgactcacgc ggctgccgct caagaagacc
      421 ggtcctctgg gtctgaagcc cttcggctac gatttgttca aggaagcacc ctcgacgttt
      481 gcgcccgtga ccaacgttcc ggtcccttcg gactacgtga tcggggcggg ggatgagctg
      541 agcatccagc tgtacggcag tcagaaccgc acgttgaagc tcaccgtcgg ccgcgatggt
      601 cgcattgcct tcccggagct cggcccgatc agtgtcgcag gcctcgtgtt cagcaaggcc
      661 aaggcggaca tcgagtcacg ggtcgagcac cagctgatcg gcgtgcgagc cagtgtcgcc
      721 atggccgaca cccgttccat ccgtgtcttc gtcctgggcg aggcgcgtcg gccaggctcc
      781 tacacgatca gcgggctggg aacgatcacc tccgcgctgt ttgccgcggg cggggtcaag
      841 cgaatcggct cgctgcggcg aatcgagctc aagcgccagg gcaccgtcgt gcgcaagctg
      901 gacgtctatg atctgctgat ccggggcgat acgaccgacg atgcgaagct cctgcagggc
      961 gatgtcatat tcgtgccgcc cgtggggccg acggtgagcg tctatggtga ggtgcgacgc
     1021 ccggccatct acgagatcaa gaacgaatcg acggtggccg atgtagttca gctcgccggc
     1081 ggactgacac cgcaagccga tccggtcacc gcgatgctca cccgcatcga cgcgaatcaa
     1141 catcgagtgg tgctgcaggc cgatatcctc gggcaggatc gcagccgggc actggtgcgt
     1201 aacggggacg agctgcgggt cacccgcctg cgcccgacgc ttgattccgc ggtggtgttg
     1261 cagggacatg ttttcaccgc cggggctttc ccctatcacg aggggatgca tatcagtgac
     1321 gtgatccact ccacggacga actgcgtccc gatgcggacc tgcattacgt tctcatccgg
     1381 cgcgagctgc cccccgatcg ccgcgtcgcc gtgctgtccg tcgatctggc ggcggcctgg
     1441 agatcacccc gatcgaacca ggacatcgtt ctcatgcccc gggaccggat cactgtgttc
     1501 gatctttcct ccggtcgcga caagatcatt cagccgctga tggatgagct gcgcaggcaa
     1561 tccagcgcga tgcagccggc cgaggtggtg cacgtggagg gcaaggtcaa ggtacccgga
     1621 gagtaccccc tgcagcccgg tatgacggtg gtcgatctga tccgggccgg aggtggcctg
     1681 gcggatgcgg ccgcctatgg cggcacggcg gagctcaccc gctatcgggt ggaaaagggg
     1741 gaaacgcgcc aggtccaact cgtcaacgtg gatctcacgg cggcgctgca cggcgatccg
     1801 agccagaacc tgaaactgga gcccttcgat gatctgagtg tcaagcaggt ctcgcagtgg
     1861 gatgttcagg aaagcgtcct gctgcgcggg gaagtgcggt tcccaggccg ctacatcatc
     1921 cgcaacggcg acacgctccg aggggtgatc gcccgggccg gcggcttgac gagctttgcg
     1981 ttcccggaag gcagcgtgtt cacccgcgag actctgaagg agctcgagca gaagcagatg
     2041 gacatgctgg ctacacgcat gcagacggcc ctcaccggat tggccatccg cggcgcggcg
     2101 gccggcgtgg gcggtggggc gagcgcgctg acgctgggtc agtcgctgct gggccagctc
     2161 cgatcggcca aggccgtcgg gcgtctcgtc atcgatctgc cgcggctgat gcgcgaacct
     2221 ccgggatcgg ccgatgatgt gatcctgcgc aatggcgatc tgctgatcgt gcccaagttc
     2281 caacagcagg tcactgtcat cggcgaggtt cagtcggcca cctcccatct gtaccggcgc
     2341 ggcctgggcc gcgatgagta catcgcactc agtggcggac tgacgcgcga tgccgataag
     2401 cgcaagatct atgtcgtacg cgccaatggc agcgtcgtcg cccgcaacgg caatcgctgg
     2461 ttccgcggca cggacgtggc gatcaagccc ggcgacacca tcgtcgttcc gctcgatacg
     2521 gaacgcatgc cgccgctgcc cttctggcag gccgtgacgg gcattctcta caacgtggcc
     2581 ctgggggcgg cagcaattcg cgtgctgtag tggatcgaaa aatcccctgg cggcgatttt
     2641 tcggatatcg gcggccgcca aggtgcgatt ttggcggctt ggatcggcga aaactccagt
     2701 gtgaacgggc agtagtgtaa atcgccgagc cgggcccgct agcactgttg agccgctgca
     2761 ttgacagtcg gccggggtct cgcataaagt tctccgttca agtgttgaac ggatactcga
     2821 tcggcacact catgagcggc tcgctgctga ccgaggagat gcgataccgg ctgttgcgac
     2881 tgatcgcggc caacccgcac atgagtcagc gcgacgtcgc gcgcgagctg ggcatcagtc
     2941 tcggcaaggt caactattgc ctgcgggcgc tggtgcagcg cggctgggtc aaggcccaca
     3001 acttcaagaa cagtcgcaac aaggccgcct acatgtacct gttgaccccg cgcggcatcg
     3061 aggcgaaggc gctgctcgcc gtgcgcttcc tgaagatcaa gaaacacgag tacgagcggc
     3121 tgcgccagga aatcgagcag atccgcatcg aggccggcga gcccggccgc cggtagcggc
     3181 ccacgggagc tgacgcatgc aaacgcaccc cgccgcaatc agggatcgac ccatccggat
     3241 cgcggtggtc ggctgcggcc gtatcgcggc caatcatttc aaggcgatcg aggctcaccg
     3301 taacgagctg cggctcgtcg cggtgtgcga caacgatccg caggccctcg aggcggcaca
     3361 gaggcgtcac gacgttccgg gctacggcag catcgtcgag ctgctcaagt ccgaggtgga
     3421 tgtcgtggcg ctgtgtacac cgagcgggtt gcatccgctg cacgcggtgc tcgcagcaag
     3481 ggcgggatgc cacgtgatca ccgagaagcc catggcgact cgctggagcg acgggctgag
     3541 catggtgcgt gaatgcgacg aggccggtgt ccatctgctg gtcgtgaaac agaaccggcg
     3601 caatgcgacg ctgcagctgc tgaagagcgc gctcgatgcc aagcgcttcg gccgcgtgca
     3661 catggtgctg atcaatgtct tctggtcgcg gccgcaatcc tattacgaca gcgcgaagtg
     3721 gcgcgggaca tgggagttcg acggtggcgc actgatgaat caggcgagtc actatgtcga
     3781 tctgatcgac tggctgatcg gccctgttga gagcgtgcag gcgtacatcg cgactctcgc
     3841 ccggaacatc caggttgagg atacggcgac cgtgggcgtg cgctggcgat cgggtgcgct
     3901 cggctcggtg aacgtcacga tgctcaccta tccgaagaat ctcgagggct cgatcacgat
     3961 actcggcgaa aaaggcaccg cgcgcattgg cggggtcgcg gtcaacgaga tccagcattg
     4021 ggagttcgag gaccgcaatc cggatgacga gcgggtcaga aacgccagct acgagaccca
     4081 atccgtgtac ggattagggc atccactgta ttacgccaat gtcgtcgatg tgctgcgcgg
     4141 caac
//
LOCUS       DASRHN010000067         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_849191, whole genome shotgun sequence.
ACCESSION   DASRHN010000067 DASRHN010000000
VERSION     DASRHN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849191"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..484
                     /locus_tag="VFB37_02575"
     CDS             149..484
                     /locus_tag="VFB37_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZO21361.1"
                     /translation="MRHSTQFIALAATTLIVSGCNLMRGNETTGQAVDDATLLTRAKA
                     ALVKDSTVSATDFNIDVYKGDVTLSGVAKTPGEIDRAIDDIKQVPGVHSVKSAARVAT
                     SDRGDGRSQ"
     gene            581..1192
                     /locus_tag="VFB37_02580"
     CDS             581..1192
                     /locus_tag="VFB37_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZO21362.1"
                     /translation="MSRKLPRNFYAREDTLAIARDLIGMHLVRALDGVRRVGRIVETE
                     AYMGPQDLAAHSARGRTQRTEVMFGPPGRAYVYFIYGFWNCMNVVTARAGIPHAVLLR
                     ALEPIEGITDKTWGPGLLCRAMRIDRTLNGADLRGASLWIEHPRAPLRAPRIGRSPRI
                     GVDYAGKWARRPWRFFDLASPYVSTAPARLRQRRSDPAALIAP"
     gene            1252..2664
                     /locus_tag="VFB37_02585"
     CDS             1252..2664
                     /locus_tag="VFB37_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZO21363.1"
                     /translation="MACAALSACATESGKSTAAPAKAATPPPAEVAPAAELYPSTYHV
                     PSSAPTLIQHATVLTGTGARLEDADVLIVDGRIQSVGPNLTAPPQAHIIEGRGRWVTP
                     GLIDVHSHLGAYPSPAVEANADGNELTSPSTANVWAEHSVWPQDPGFRTALAGGITTL
                     QILPGSGNLFGGRSVVLKNVPSVTYQGMKFPGAPQGLKMACGENPKRVYGEKHQFPST
                     RMGNVAGYRAQWVEAKEYLREQQEYQKKLAAGDKDAKPPKRDLRLDTLAAVLKGDILV
                     HMHCYRADEMATILDMSKEFGYHVTAFHHAVEAYKIADLLAENGVCAAMWADWWGFKM
                     EAYDGIPENIAFVDAAPGGCAIVHSDSEEGIQRLNQEAGKAIAYGRRAGLDIPPERAI
                     RWLTSNPAKALGLLERTGTLEPGKMGDLVVWNGSPFSVYALADYVLIDGAVRYDRSNP
                     AVAPQSDFLLGQPAVDGVYP"
     gene            2661..3938
                     /locus_tag="VFB37_02590"
     CDS             2661..3938
                     /locus_tag="VFB37_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO21364.1"
                     /translation="MSARNSLPAIVLALFPLTVAAAASSTVLIRNATVHTLGTPAVLE
                     HTDLLIENGRIAGWGHDLKAPADAETIDAGGKPVTPGLFGGLGHLGLEEIGLEPSADD
                     YALRLGSMRPEFDVTPAFNPQSIVLGVSRLGGITFAVLAPSSGPGHSGGAGGTIIAGQ
                     GSVAQLDGTVVPKVRALFIDVGGDANALSGGSRAAQFMLLQQAISEVHSPKELLPNDQ
                     RLLTPAGRRALQSFLDGAGPVVFDVDRASDIREVIAFANREKLHAVIKGATEAWRVAP
                     ELAAAHIPVILNPLDDLPESFDSVGATLENAARLNRAGVKIAFSLDAAQPHNMRKVRQ
                     TAGIAVAHGLPWEVALAALTRNPAEIFGVTAGHGSLERGRPADLVLWSGDPLEVTSLA
                     ERVFIAGHEQSMQSRQTLLRDRYLAKLRANAAR"
     gene            3925..4680
                     /locus_tag="VFB37_02595"
     CDS             3925..4680
                     /locus_tag="VFB37_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZO21365.1"
                     /translation="MLRAEAEAQASSRGPSRPQEAQKNFKYYDLLLGAYVCVLLCANL
                     IGPAKVSTIQVPGLGPVTFVAGVLFFPFSYFFGDVLTEVYGYARDRRVVWSGFGALVF
                     ASVMATVIVHLPPADFWKAQQPAVEAVFGNTPRIIAASIIAFWCGSFVNSYVLANMKL
                     LTRGRWLWTRTIGSTICGELVDSGLFYTIAFAGIWSTKQLVSVGITQYVLKSTWEVVM
                     TPATYKIVGFLKRAEQEDYYDRGTNFTPFSLKA"
BASE COUNT          831 a         1587 c         1564 g          845 t
ORIGIN      
        1 cgcgacttcg cgccctgcgg gatcctgcca gggtgccttg ctcgcctagc cctgcacctc
       61 tcggcatcgc atgcgccggg cccgggcatc cgtcttgctg cgctacaccg acgacacggc
      121 aacgcgcgaa atttcgggag cacgccccat gcgacattcc acccagttca tcgccctggc
      181 ggcgaccacc ctgatcgtct cgggttgcaa tctgatgcga ggcaatgaga cgaccggtca
      241 ggctgtggac gatgccaccc tcttgacgcg agcgaaggcc gccctcgtca aggacagcac
      301 cgtctcggca acggacttca acatcgacgt gtacaagggc gatgtcacgc tctccggcgt
      361 cgccaagaca cccggcgaga tcgaccgcgc catcgatgac atcaagcagg tgcccggcgt
      421 gcacagcgtg aagagcgctg cccgcgtggc gacctccgac cgaggcgacg ggcgatctca
      481 gtaaccggct cggccggtat ctcctgcgta ttgcgcctcg gccctggggg gcgttctgcg
      541 cgtgcgcgcc atcctctgag gtgatagtta tacgcggctg atgagtcgca agctgccgcg
      601 aaacttctat gctcgggaag acaccctcgc cattgcccgt gatctgattg gcatgcacct
      661 cgtgcgtgcg ctcgatggcg tgcgcagagt gggtcgcatc gtggaaaccg aagcgtacat
      721 gggcccgcag gacctggccg cgcattccgc gcgcggccgc acgcagagaa cggaagtcat
      781 gttcggccct cccggccgcg cgtacgtcta cttcatctac ggattctgga actgcatgaa
      841 cgtcgtaacg gcgcgtgcgg gtattccgca cgcggtcctg ctgcgcgcgc tcgagccgat
      901 cgagggaatc accgacaaga cctggggtcc gggattgctg tgccgagcca tgcgcatcga
      961 ccgtactctg aacggcgctg acttacgtgg cgcaagcctg tggatcgagc acccgcgcgc
     1021 gccgctgcgc gcgccgcgaa tcgggcgcag cccgaggatc ggggtggact acgccggcaa
     1081 gtgggcgcgg cgtccctggc gcttcttcga tctcgcatcg ccgtacgtct ccacggctcc
     1141 cgctcgcctg cggcagcggc gaagtgatcc cgcggccctc attgcgccat aatgcgcgga
     1201 ttgcgcgccg cgcacgaagg gatgttcgtg atgagaagaa taatttgttt tatggcctgc
     1261 gcggcactca gcgcctgtgc gacggaaagc ggcaaatcca ctgcagcgcc cgcgaaggct
     1321 gcgacacccc ctccggccga ggtagccccc gcagccgagc tgtatccctc cacttaccac
     1381 gtacccagta gcgccccgac gctgattcag cacgcgacgg tcctcacggg caccggtgcg
     1441 cggctggagg atgccgatgt cttgatcgtg gacggcagga ttcagtccgt gggccccaat
     1501 ctgaccgcgc caccccaggc gcacatcatc gagggcaggg gccgttgggt gacacccggc
     1561 ctgatcgacg tgcactctca tctgggggcg tatcccagcc ctgcagtgga agccaacgct
     1621 gatggcaacg agctcacctc gccctccacc gccaatgtgt gggccgagca ttccgtgtgg
     1681 ccccaggatc cgggctttcg caccgcgctc gccggtggca tcaccacgct gcagattctg
     1741 cccggctcgg gaaatctctt cggcggccgc tcggtggtgc tgaagaacgt tcccagcgtg
     1801 acgtatcagg gaatgaagtt tccgggtgcc ccgcaagggc tgaagatggc gtgcggcgaa
     1861 aatcccaagc gcgtgtacgg tgagaagcat cagtttccct cgacccgcat gggtaacgtt
     1921 gccggctatc gggcacagtg ggtggaggcg aaggagtacc tgcgcgagca gcaggaatac
     1981 cagaagaagc ttgccgcggg tgacaaggat gcgaagccac cgaaacgtga tctgcggctc
     2041 gacaccctgg cggcggtgtt gaagggtgac atcctcgtgc acatgcattg ctaccgcgcg
     2101 gatgagatgg cgaccatcct cgacatgtcg aaggagttcg gctaccacgt caccgccttc
     2161 caccacgcgg tcgaggctta caaaattgcg gatcttctgg cggagaacgg agtctgcgcg
     2221 gccatgtggg ccgactggtg gggcttcaag atggaagcct acgatggcat cccggagaac
     2281 atcgcatttg tcgatgccgc tccgggcggc tgcgccatcg tgcactccga ctcggaggaa
     2341 gggattcagc gactgaacca ggaggcgggc aaggcgatcg cctatggccg acgcgccggg
     2401 ctcgatattc cgcccgagcg cgccatcaga tggctgacgt ccaatccggc gaaggcactc
     2461 gggctgctcg agcgcacggg cacgctcgag ccgggaaaga tgggtgacct cgtcgtctgg
     2521 aacggcagtc cgttcagtgt ctacgccttg gcggactatg tgctgatcga cggtgcggtg
     2581 cgctacgacc gctccaatcc ggccgtcgct ccgcaatcgg acttcctgct gggccaaccc
     2641 gctgtcgacg gggtgtaccc atgagcgcgc gcaactccct gccggcgatc gtgctcgctc
     2701 tgtttcccct gaccgtcgcc gccgcagcct cgagcaccgt gctgatccgc aacgcgaccg
     2761 tacacacgct tgggactccc gcagtactcg agcatacaga cctgctcatc gagaacggcc
     2821 gcatcgccgg atggggacac gatctgaaag cccccgctga tgcggaaacg atcgatgcgg
     2881 gcggcaagcc cgtcacgccc ggcctgttcg gcggcctcgg gcatctggga ctcgaggaga
     2941 tcggcctcga gccctccgcg gatgattacg cattgcgact cggctcgatg cggcccgagt
     3001 ttgacgtgac gcccgccttc aatccgcagt ccatcgtgtt gggcgtgagt cgcctcggcg
     3061 gtatcacctt tgccgtgctg gcgccgagct cgggtccggg gcactcgggc ggtgcgggtg
     3121 gcacgatcat cgccggacag ggatcggtcg cgcagctcga cggaacggtc gtaccgaagg
     3181 tgcgggcgct gttcatcgat gtggggggtg atgccaacgc gctctccggc ggcagccgcg
     3241 ctgcgcagtt catgttgctt cagcaagcaa tcagcgaggt ccactcgccg aaggagctgc
     3301 ttccgaacga tcagaggctg ctcacgcccg cggggcgccg ggcgctgcag agtttcctcg
     3361 atggcgcggg gccggtggtg ttcgatgtgg accgcgcctc ggacatccgt gaggtcatcg
     3421 cgttcgctaa tcgcgagaag ctgcacgcgg tgatcaaggg agccaccgag gcctggcggg
     3481 tcgccccgga gctcgccgcc gcgcacattc ctgtcatcct caatccgctc gacgatctgc
     3541 cggaaagctt cgacagtgtc ggtgcgacgc tggagaacgc ggcgcgcttg aaccgggccg
     3601 gcgtcaagat tgcattcagc ctcgacgctg cgcagcctca caacatgcgc aaggtgcgcc
     3661 agacggccgg gatcgccgtc gcccacggat taccgtggga agtggccctc gccgcgctca
     3721 ccaggaaccc tgccgagatc ttcggggtca cggccggcca cggctcactc gagcgcggcc
     3781 ggcccgccga tctggtgctc tggagcggcg accccctcga ggtcacgagc ctcgctgagc
     3841 gcgtcttcat cgcaggacac gagcaatcga tgcaatcgag gcagacgctg ctgcgtgatc
     3901 gctatctggc gaagttgcgt gccaatgctg cgcgctgagg cagaggcaca ggcgagctcc
     3961 cgagggccga gccggccaca agaggcgcag aagaacttca agtactacga cctgctgctc
     4021 ggggcatacg tctgcgtcct gctgtgcgcg aacctcatcg ggcccgccaa ggtatccacg
     4081 attcaggtgc cgggcctcgg cccggtcacc ttcgtcgccg gcgttctctt ctttcctttc
     4141 tcctatttct tcggggatgt cctcaccgag gtctacggct atgcgcgcga ccggcgggtg
     4201 gtgtggagcg gcttcggagc gctcgtgttc gcctccgtga tggcgaccgt catcgttcac
     4261 ctgccacccg cggacttctg gaaggcgcag cagcccgccg tcgaggctgt attcggtaac
     4321 acaccacgca tcatcgcggc atcgatcatc gcattctggt gcggctcctt cgtcaacagc
     4381 tatgtcctgg cgaatatgaa actgttgacg cgtggacgat ggctgtggac tcgcaccatc
     4441 ggctcgacga tctgcggaga gctcgtggac tccggcctgt tctacacgat cgccttcgca
     4501 gggatctggt ccacgaagca gctggtcagc gtcggcatca cgcagtacgt gctcaagtcc
     4561 acgtgggagg tcgtgatgac gccggccacc tacaagatcg tcggcttcct caagcgcgcc
     4621 gagcaggagg attactatga ccggggcacc aacttcacgc ctttctcgct gaaggcctga
     4681 aggcttgcgg agagctggcg ccgtcacccg ggcgcaaaga aggaagcagt tactgccatc
     4741 caccacaggg cccctggcga gggggtccta ccccgcgcca gacaccggga gacgctagat
     4801 ccctatcgtc cggttagcgg tctgcac
//
LOCUS       DASRHN010000068         7357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1012494, whole genome shotgun sequence.
ACCESSION   DASRHN010000068 DASRHN010000000
VERSION     DASRHN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012494"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /gene="hutU"
                     /locus_tag="VFB37_02600"
     CDS             complement(<1..1629)
                     /gene="hutU"
                     /locus_tag="VFB37_02600"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZO21366.1"
                     /translation="MSDTPARPPLRAPHGSTLSCRSWITEAALRMLLNNLDPEVGERP
                     ADLVVYGGIGKAARNWTCFEQIVAALRRLGSEETLLIQSGKAVGVFRTHADAPRVLMA
                     NSNLVARWADWDHFNELDRKGLMMFGQMTAGSWIYIGSQGIVQGTYETFAEMGRQHFG
                     GRLAGKWILTAGLGGMGGAQPLAATMAGASMLAVECRPERIAKRLQTRYLDVEAANLE
                     EVVEILERARREGRPISVGLLGNACEVLPEILRRGIRPDAVTDQTSAHDPVNGYLPRG
                     WSLEQWDRLRAEDPKRVASEARTSMARHVESMLEFKRLGIPVFDYGNNLRQVAFDEGV
                     KDAFAFPGFVPAYIRPLFCRGVGPFRWAALSGDPEDIYRTDAKVKELLPNDTHLHHWL
                     DMARERIQFQGLPARICWVGLGDRHRLGMAFNEMVARGELRAPIVIGRDHLDSGSVAS
                     PNRETEAMRDGSDAVADWPLLNALLNTASGASWVSIHHGGGVGMGYSQHAGVVIVCDG
                     SPAASRRLERVLWNDPASGVMRHADAGYESAIACA"
     gene            complement(1663..2388)
                     /gene="hutC"
                     /locus_tag="VFB37_02605"
     CDS             complement(1663..2388)
                     /gene="hutC"
                     /locus_tag="VFB37_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HZO21367.1"
                     /translation="MPAPPATATARYLQVKQHILRQISGGLLRAGERVPSENQLVREL
                     DVARMTANRALRELAADGVLVRVAGVGTFVADARVHAHPLEVRNIAEEIRARGHEYAS
                     RMVSLGPVIASRELAERCGVSPGARLDHSLLVHFEGGSPLQVEDRYVNPIVVSGYLRN
                     DFARLTPHEFLMQAAPLQRAEHTVRAIVADGRIRRLLKLEPNDACLLIRRRTWSDGQV
                     ASLADLYHPGSRYELTGAFPARS"
     gene            2747..3199
                     /gene="rimP"
                     /locus_tag="VFB37_02610"
     CDS             2747..3199
                     /gene="rimP"
                     /locus_tag="VFB37_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZO21368.1"
                     /translation="MTVTLRERLIALIEPLLAQLGYELVELEQTSGRSQALVRLFIDR
                     PEGVGVDDCERVSREVAALLDVQDPIPTAYTLEVSSPGFDRVLRTAGHFERFVGSRVF
                     VELKEPRAGRRRFTGLLQLVSAQGIELEVDRQKVEIPFAEIAKARLAV"
     gene            3264..4760
                     /gene="nusA"
                     /locus_tag="VFB37_02615"
     CDS             3264..4760
                     /gene="nusA"
                     /locus_tag="VFB37_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZO21369.1"
                     /translation="MNKEILMVVDAVSNEKGVDKEVIFEALEAALASATRKKHGEEWD
                     VRVAIDRKSGDYETFRRWKIFADDSKELEVPERELRLEDGHDIDPAVEVGGFVEQPME
                     SVAFGRIAAQQAKQVIVQKVREAERAQVVDAYRDRVGQLVSGVVKRVDRNGIYVDLGS
                     NAEGFVPRTDMIPREQVKPQDRIKAFLKEVRSEPRGPQLFLTRTAPEFLIELFKLEVP
                     EVGQGLIQILGAARDAGVRAKIAVRSNDPRIDPVGACVGMRGSRVQAVSNEIAGERVD
                     IIPYDDNPAQFVINAMSPAEVMSIVVDEDSHSMDIAVTEEKLSQAIGRGGQNIRLASQ
                     LTGWDLNVMTESDAEAKSETEAKELVQNFMRQLDVDEDVATILAQEGFSTIEEIAYVP
                     QAELAAIEEFDEDIVKELRNRARDVLLTQAIASEESLDQSMPADDLLLLEGMSPDLAL
                     ALARRGVRTREDLAEQSVDDLSDIDGLASEEAGKLIMTARAPWFEAGQ"
     gene            4781..>7357
                     /gene="infB"
                     /locus_tag="VFB37_02620"
     CDS             4781..>7357
                     /gene="infB"
                     /locus_tag="VFB37_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZO21370.1"
                     /translation="MAEVTVSQFAEVLKVPVDRLLVQLDQAGIKVDGPDARISDDAKL
                     ELLTHLRRSHGSDDGGDAAPRKITLRRKTQSELKLASAQGRARTVNVEVRRQRTYIKR
                     DVLQEQARQQQDEIDQKRREAEEAARRESERIENERRERERLESENRRRAEEEQARKK
                     AQEDARRVAEQRAREDAERREREREEEAQQQERRNFEAPREKARAAAPAPQAAPAASV
                     AAAAASPSAREKPAAAPAAPVAVGDDRGSTRYGRQELHVTGDVSSRYKKKRRTKSRPL
                     PASAEGRHAFEMPTAPVVREVSIGETVTVAELAQRMAVKATEVIKVLMNIGVMATINQ
                     PIDRDTAVLVVEEMGHTPKVLKENQIEEDLQGVVSEEASTEPRPPVVTVMGHVDHGKT
                     SLLDYIRSTKVAAGEAGGITQHIGAYHVETPKGVITFLDTPGHAAFTAMRARGARATD
                     VVVLVVAADDGVMPQTVEAIQHARAAGVPIVVAVNKVDKSDADPDRVRTELSKQEVIP
                     EEWGGQNIFVNVSARTGAGIDELLDAILLQAEVLELRAPQTGLASGVVIESSVEKGRG
                     AVATVLVKRGTLRMGDPILAGTEFGRVRAMFDETGRAVQDAAPSMPVVVLGLASPPNA
                     GDDLLVVESERKAREVALYRQGKFRDVKLARQATRAEDVFSQMGEAKAGVVAVLIKAD
                     VQGSAEALREALAKLSTDEVQVKVLGSGLGGITVSDVQLAAASKALIIGFNVRADAAA
                     RDALKESGVGVRYYSIIYEAIDDVKQMMSGMLQPEIKESILGVAQVREVFRSSKFGVV
                     AGCLVTEGLVRRNNPIRVLRDNVVIFEGALESLRRFKDDAGEVRAGTECGIGVKNYQD
                     VRV"
BASE COUNT         1315 a         2269 c         2604 g         1169 t
ORIGIN      
        1 ggcgcaggcg attgcggact cgtatccggc atcggcgtgg cgcatgacgc cgctggccgg
       61 gtcgttccac aacacacgct cgaggcgccg actggcggcg ggggatccat cgcacacgat
      121 gacgacgccg gcgtgctgcg aatagcccat tccaacaccg ccgccgtggt ggatcgatac
      181 ccagctcgcc ccgctggcgg tgttgagcag ggcattgagc agtggccagt cggctaccgc
      241 gtccgagccg tcgcgcatcg cctccgtctc gcgattcgga ctggccacgg agcccgagtc
      301 cagatgatcg cggccgatga cgatgggggc gcgcagctcg ccgcgcgcga ccatctcgtt
      361 gaaggccatc ccgaggcggt ggcggtctcc gagcccgacc cagcagatgc gcgcgggaag
      421 accctggaac tggatgcgct cgcgtgccat gtcgagccag tgatgcagat gcgtgtcgtt
      481 cgggagcagc tccttgacct tcgcatccgt gcgatagatg tcctcggggt ctccggagag
      541 tgctgcccag cggaagggcc ccacaccccg gcagaacagc ggtcggatgt aggccggaac
      601 gaaaccgggg aaggcgaacg cgtccttcac tccttcgtcg aatgcgacct ggcgcagatt
      661 gttgccgtag tcgaataccg ggatgcccag tcgtttgaat tcgagcatcg attccacgtg
      721 ccgggccatg gaggtgcggg cctcggaagc aacccgcttg gggtcttccg cgcgcagccg
      781 atcccactgc tccaggctcc agccccgagg caggtagcca ttcaccgggt catgcgccga
      841 ggtctgatcg gtgacggcat cggggcgaat tccacggcgc aggatctccg ggagcacttc
      901 gcaggcattc cccagcaagc cgacggagat gggccgccct tcgcgacggg cacgctccag
      961 gatctcgacc acttcctcca ggttggccgc ctcgacatcc agatagcggg tctgcaggcg
     1021 cttggcgatt cgctcgggac ggcattccac cgccagcatg gaggccccgg ccatggtggc
     1081 cgccaggggc tgggccccgc ccatgccgcc gagacccgcc gtgagaatcc acttgccggc
     1141 gagccgcccg ccaaaatgct gccggcccat ctcggcgaag gtctcgtagg tcccctgcac
     1201 gatcccctgg cttccgatgt agatccagga cccggcagtc atctgcccga acatcatcag
     1261 gcccttgcga tcgagctcat tgaagtgatc ccagtccgcc cagcgcgcaa cgaggttgga
     1321 gttggccatc agcacccgcg gcgcatcggc atgcgtgcga aaaaccccga ccgccttgcc
     1381 ggactggatg agcagcgtct cctccgagcc gagccggcgc agagccgcga cgatctgctc
     1441 gaaacaggtc cagttgcgcg cagccttgcc gatgcccccg taaacgacca ggtccgcggg
     1501 gcgctcgccc acttccgggt ccaggttgtt cagcagcatg cgcagcgcgg cctcggtgat
     1561 ccagctacga caactcaacg tggagccgtg cggggctcga agaggtggac gggcgggggt
     1621 gtcggacatc gttcgctctc caggtaacag tcgggcgcgc ggtcagctgc gcgccgggaa
     1681 ggctcccgtc aactcgtagc gggagcccgg gtggtacaga tccgcgaggc ttgcgacctg
     1741 gccgtcactc caggtgcgtc gtctgatcag caggcatgca tcattcggct ccagcttcag
     1801 cagccggcgg atccgcccat cggccacgat ggcgcgcacc gtatgctcgg cgcgttgcaa
     1861 gggtgcagcc tgcatgagga attcatgcgg ggtcagccgc gcgaaatcgt tgcgcaggta
     1921 gccggacacg acgatcgggt tgacgtaccg gtcctcgacc tgcagcggcg atccgccttc
     1981 gaagtgcacc agcagggaat ggtcaaggcg cgctccgggg ctgacaccac agcgttcggc
     2041 gagctcgcgc gaggcgatca ccggaccgag cgaaaccatc cggctcgcat actcgtgacc
     2101 gcgcgcgcgg atctcctcgg cgatgttcct cacctcgagg gggtgggcat gcacgcgagc
     2161 atcggccacg aacgtgccca cgccagccac cctgacgagg acgccatcgg cggcgagctc
     2221 gcgcagcgct cggttggcag tcatgcgggc cacgtccagc tcgcggacga gctgattctc
     2281 tgaaggcacc cgctcccctg cgcgcagcag accgccggag atctgccgca ggatgtgctg
     2341 cttcacctgc aggtaacggg cggtggcggt cgcgggcggg gcaggcattc gaagcttcac
     2401 gggtgcgtgg ttgcacgagc ttgacacgcc gaggccatcc tgtctataca ggtctaatcc
     2461 gcatgcggct cgcactacgg cgggtgcgca gcggtgcatt cgcacgcatg tttcgcggac
     2521 gtgcgggttt gggtagaatg cgcgcccccg cgggcctcgg ccgtcggggg gcgggcgcac
     2581 caaggcgacg gggacctggc gggacctgtt gctcgagcgg caggtactca ggcacccgta
     2641 cgaagcgctt gttcaagaag ccccccgagg ggctttttgc atttttggag tcggtgtgat
     2701 gggcccttgg aaggcccatt tttattcgtg gcggcgggta cttgcgatga cggtcacctt
     2761 gcgggagcgg ctcatcgccc tcattgagcc tctgctcgct caactgggtt atgagctggt
     2821 cgagctcgag cagacatcgg ggcgttcgca ggcgctcgtg cgcctgttca tcgatcggcc
     2881 ggagggggtg ggggtagacg attgcgagcg cgtgagccgc gaggtcgctg ccctgctcga
     2941 tgtgcaagac ccgattccga ccgcttacac actggaggtg tcctcgcccg gattcgaccg
     3001 ggtcctgcgc acggcggggc acttcgagcg cttcgtgggc tcgcgggtgt ttgtggagct
     3061 gaaggagccg cgggccgggc ggcggcgctt taccggattg ctgcagctcg tgagcgcaca
     3121 ggggatcgag ctcgaggtgg acaggcagaa agtcgagatt ccgttcgccg agattgccaa
     3181 ggcgcgactg gcggtttgac gggcatgacg cgcggccgca aggccggaag ctgggggcct
     3241 tgagccccgt gaggatcgaa agcgtgaaca aagagattct gatggtggtc gatgccgtct
     3301 ccaacgagaa gggcgtggac aaggaagtca ttttcgaggc cctcgaggcg gcgctcgcct
     3361 ccgcgacgcg caagaagcac ggcgaggaat gggacgtgcg ggtggccatc gaccgcaaga
     3421 gcggcgatta cgagaccttt cgtcgctgga agatctttgc cgatgactcg aaggagctgg
     3481 aggtgcccga acgcgagctg cgtttggagg acggccacga catcgacccg gcggtcgagg
     3541 ttggcggatt cgtcgagcag ccgatggaat cggttgcctt cggacgcatt gccgcccagc
     3601 aggcgaagca ggtcatcgtg caaaaggtgc gcgaggcaga gcgtgctcag gtggtggacg
     3661 cctatcgcga ccgggtcgga cagctcgtca gcggagtggt gaagcgagtg gatcgcaacg
     3721 ggatctacgt cgatctcggc agcaacgcgg aaggcttcgt gccgcgcacc gacatgattc
     3781 cgcgcgagca ggtgaaaccc caggatcgca tcaaggcctt cctgaaggaa gtgcgctccg
     3841 agccgcgcgg tccgcagctc tttttgaccc gcacggcccc ggagttcctc atcgagctgt
     3901 tcaagctgga ggtgccggag gtgggccagg gcctcattca gatcctcggc gcggcgcgtg
     3961 atgcgggtgt gcgcgccaag atcgcggtgc gcagcaacga tccgcgcatt gatccggtcg
     4021 gtgcgtgcgt cggcatgcgc ggctcgcgcg tgcaggcggt ctcgaacgag atcgccggtg
     4081 agcgtgtgga catcattccc tatgacgaca atccggcgca gttcgtcatc aatgcgatgt
     4141 ccccggcgga agtcatgtcg atcgtggtgg acgaggactc gcacagcatg gatatcgccg
     4201 tcaccgagga gaagctctcg caggccatcg gccgcggcgg acagaacatt cgactcgcca
     4261 gccagctcac cggctgggac ctgaatgtca tgaccgagtc cgatgccgag gccaagagtg
     4321 agaccgaagc gaaggagctg gtgcagaact tcatgcgaca gctcgacgtg gacgaggacg
     4381 tggcgacgat tctggctcag gagggttttt cgactatcga agagatcgct tacgtgcctc
     4441 aggccgagct ggcggcgatc gaggagtttg acgaagacat cgtcaaggag ctgcgcaacc
     4501 gcgcgcgcga tgtgctgctg acgcaggcca tcgcgagcga ggaaagcctc gatcagtcca
     4561 tgcctgccga cgacctgctg ctgctcgagg gtatgagccc ggatctggcg ctcgcactcg
     4621 caaggcgcgg agtgcgcacc cgcgaggacc tcgccgagca gtccgtggac gacctgtccg
     4681 acatcgacgg cctcgcctcg gaagaggccg gcaagctgat catgaccgca cgcgccccct
     4741 ggttcgaggc gggccagtga ggccggaata ggattgatct atggcagaag taacggtttc
     4801 ccagttcgct gaagtgctga aggtcccggt ggatcggctg ctcgtgcagc tcgatcaggc
     4861 gggcatcaag gtggacggac ccgatgcgcg catcagcgat gatgcgaagc tggagctgct
     4921 cacgcatctg cggcgcagcc acggcagcga cgacggcggc gatgccgcgc cgcgcaagat
     4981 taccttgcgc cgcaagaccc agagtgagtt gaagcttgcg agcgcacagg gccgcgcgcg
     5041 caccgtcaac gtggaggtgc gccgccagcg cacctacatc aagcgtgatg tgctgcaaga
     5101 gcaggctcgc cagcaacagg acgagattga tcagaagcgc cgcgaggcgg aggaggccgc
     5161 acggcgcgaa agcgagcgca tcgagaacga gcgtcgtgag cgcgagcggc tcgagagcga
     5221 gaatcgccgc cgcgcggaag aggaacaggc ccgcaagaag gcccaggaag atgcccggcg
     5281 ggtggccgaa cagcgggcac gcgaggacgc cgagcgccgc gagcgcgagc gcgaagaaga
     5341 ggcccagcag caggagcggc gcaatttcga ggcgccgcgc gagaaagcgc gcgcggccgc
     5401 gcctgctccg caggcagccc cggccgccag tgtggcggcc gcagcagcct ccccatccgc
     5461 acgcgagaag ccggcggcgg ccccggcagc tcctgttgct gtgggcgatg atcgcggttc
     5521 gacacgctat ggccgccagg agctgcatgt cacgggagac gtgagctccc ggtacaagaa
     5581 aaagcggcgc acgaagtcgc gtcccttgcc ggcgagtgcc gaggggcgcc atgccttcga
     5641 gatgccgacc gcgcccgtgg ttcgggaagt cagtatcggg gagaccgtca cggtggccga
     5701 gcttgcgcag cgaatggctg tcaaggcgac tgaggtcatc aaggtgctga tgaatatcgg
     5761 ggtcatggcc acgatcaatc agcccatcga tcgcgacacc gccgtgctgg tggtcgagga
     5821 gatgggtcat accccgaagg tgctgaagga aaaccagatc gaggaggatc tgcagggggt
     5881 ggtcagcgag gaggcgagca ccgagccgcg accgccggtg gtgaccgtca tgggccacgt
     5941 tgaccacggc aagacctcgc tgctcgatta catccgcagc acgaaggtcg ccgccggcga
     6001 ggccggcggc atcacgcagc acatcggtgc ctaccatgtc gagacgccga agggcgtgat
     6061 cacttttctg gatacgccgg gccacgcggc gttcacggcc atgcgcgcgc gcggtgcccg
     6121 ggcaaccgat gtggtggtgc tggtcgttgc cgccgatgac ggggtcatgc cccagaccgt
     6181 cgaggcgatt caacatgcgc gcgctgcggg agtgccaatc gtcgtcgcag tcaacaaggt
     6241 cgacaagagc gatgcggatc ccgatcgcgt gcgcacggag ctctccaagc aggaagtcat
     6301 ccccgaggag tggggcggtc agaacatctt cgtcaacgtc tccgcccgga ccggggcggg
     6361 tatcgatgag ctgctcgatg cgatcctgct gcaggcggaa gttctcgagc tgcgtgctcc
     6421 gcagaccggg ctggcttccg gcgtggtcat cgaatccagc gtcgagaagg gacgcggcgc
     6481 agtcgcgacg gtgctcgtga agcgcggcac gttgcgcatg ggagacccga tcctcgccgg
     6541 cacggagttc ggccgggtcc gcgccatgtt cgacgagacc ggacgggcgg tgcaggacgc
     6601 cgccccttcc atgcccgtcg tggtgctcgg actggcgagt ccccccaatg ctggagacga
     6661 tctgctggtc gtcgaaagcg agcgcaaggc gcgcgaggtg gccctgtatc ggcagggcaa
     6721 attccgcgac gtgaagctcg ctcgacaggc gactcgcgcc gaagacgtgt tctctcagat
     6781 gggcgaggcg aaagccggcg tcgtggccgt gttgatcaag gcggacgtgc aaggcagcgc
     6841 cgaggcgctg cgcgaggcgc tggccaagct gtccaccgat gaagtccagg tcaaggtgct
     6901 gggcagcgga ctgggcggca tcaccgtctc ggacgtgcag ctggccgcag cctccaaggc
     6961 gctcatcatc ggcttcaacg tgcgtgcgga tgccgctgcc cgtgatgcgc tgaaggagag
     7021 cggagttgga gtccgttact acagcatcat ctatgaggcc atcgacgatg tgaagcagat
     7081 gatgtcgggc atgctgcagc cggaaatcaa ggaatcgatc ctgggtgtgg cgcaggtgcg
     7141 cgaggtattc cgctccagca agttcggtgt ggtggccggg tgtctggtca ccgaagggct
     7201 ggtccgccgc aacaatccca tccgcgtgtt gcgtgacaac gtggtgatct tcgagggcgc
     7261 gctcgagtcg ctgcggcggt tcaaggacga tgccggcgag gtgcgggcgg gtaccgagtg
     7321 tggcatcggc gtgaagaact accaggacgt gcgggtc
//
LOCUS       DASRHN010000069        16274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_190127, whole genome shotgun sequence.
ACCESSION   DASRHN010000069 DASRHN010000000
VERSION     DASRHN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190127"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFB37_02625"
     CDS             complement(<1..234)
                     /locus_tag="VFB37_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21371.1"
                     /translation="MSEHFEALVTATYGRHLLVRDRAGRELKARPFGRGHVVVCGDHV
                     SCRIDPRSADIHVFDVHPRHGALYRSNARGGAEP"
     gene            291..851
                     /gene="orn"
                     /locus_tag="VFB37_02630"
     CDS             291..851
                     /gene="orn"
                     /locus_tag="VFB37_02630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008048058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZO21372.1"
                     /translation="MPSGENLIWIDLEMTGLRPDVDSIIEIATVVTDKELTVIADGPV
                     LAIHQPQEVLDRMDEWNQKQHGSSGLTARVRSSRISEAEAEERTLEFLAPLVTGGASP
                     MCGNSICQDRRFLARHMPRLERYFHYRNLDVSTLKELARRWAPAVAASFLKQGTHLAH
                     ADIQESIRELRHYRTHLLAPAYAARQ"
     gene            938..1828
                     /locus_tag="VFB37_02635"
     CDS             938..1828
                     /locus_tag="VFB37_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZO21373.1"
                     /translation="MSSPRLDERAGCRVVFKCENLQHGGAFKYRGALNAILPLAVAGN
                     VAAVATHSSGNHGTAVALAARVCGLDAHVVVPRDSARPKIAAIERAGGHVILCEPGQP
                     AREAALADLLREVDAHVVPPFDDARVIAGQGTAALELFSEEPGLDLVSTPIGGGGLIG
                     GTALAAQALAPRCRVVGAEPAGADDAYRSFRSGTRQPVVKPQTIADGLRGSIGALNFE
                     LLRRHVADVVTVSEREIVEAMRIVLQDLKLLIEPSSAVPVAALLAGKLGSAGQRVGII
                     LSGGNVDLEQCPFLRGERLA"
     gene            1825..2226
                     /locus_tag="VFB37_02640"
     CDS             1825..2226
                     /locus_tag="VFB37_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZO21374.1"
                     /translation="MTEELLTHRGGCHCGAVAFEVLAPARLRASDCNCSICRMSGYLH
                     LIVPQGRFRLLRGAELLTEYRFNTGTARHLFCARCGVKSFYVPRSNPDGYSINVRCLD
                     PSTVAHVEVEPFDDDDREAATARIRELSRQG"
     gene            complement(2248..4623)
                     /gene="ppsA"
                     /locus_tag="VFB37_02645"
     CDS             complement(2248..4623)
                     /gene="ppsA"
                     /locus_tag="VFB37_02645"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZO21375.1"
                     /translation="MNRYVIPFERLAMRDVETVGGKNASLGEMIGSLASLGVRVPGGF
                     ATTAQAYRDFLKQGDLEGRIRAELASLNVDDVTRLAATGARIRGWIMATPFHPELQKA
                     VAEELRRMSGGSEIAVAVRSSATAEDLPEASFAGQQETFLNVRGEANVLRAMHEVFAS
                     LFNDRAISYRVHQGFDHNAVALSAGVQHMVRSDLGSSGVMFTLDTDSGFRDVVFITAS
                     YGLGETVVQGAVNPDEFYVYKLGLRAGKYPILRKNLGGKAVKMVYAPEGSSERVRTVE
                     VPQHERMRFCLEEADLLALARQALLIEDHYKAPMDIEWGKDGASGEIFILQARPETVQ
                     SRAGRTIQRFSLKSRSRVLTSGRSIGQRIGAGPARVIKDASEMARVQNGDVLVADMTD
                     PDWEPVMKRASAIVTNRGGRTCHAAIIARELGIAAVVGCTDATQSIREGQDVTVSCAE
                     GDTGYVYEGMLEFERKQIELDTLPKIPVKIMMNVGNPDRAFDFAGIPHRGVGLARLEF
                     IINRMIGVHPRALLEFDQLDSELQEQIRGQMAGYADPVGFYVDKLAEGIAQIAAAFAP
                     EPVIVRLSDFKSNEYANLIGGARYEPEEENPMLGFRGASRYVDETFRPCFELECLALK
                     KVRDEMGLTNVQVMVPFVRTLKEARQVTELLAENGLERGTGDLRIIMMCELPTNALLA
                     DEYLNHFDGMSIGSNDMTQLTLGLDRDSSIVAKAFDERDEAVKMLLAMTIAACKRRGK
                     YVGICGQGPSDHPELARWLLEQGIESMSLNPDTVVETWMFLAESAGKPAKG"
     gene            4719..5540
                     /locus_tag="VFB37_02650"
     CDS             4719..5540
                     /locus_tag="VFB37_02650"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HZO21376.1"
                     /translation="MSRRTVFFVSDQTGVTAETMGHSLMTQFEGLEFRPVTLPFVSSV
                     DKAHEAVRRINRAAEEEGLRPIIFSTLVQDELRDIVVSANGLFLDFFAAFVGPLEREL
                     NTRSTHRAGRAHGMADVAAYTARINATNFALANDDGTGGDYAHADVILLGVSRSGKTP
                     TCLYMALQYGVFAANYPLTEEDLEAGRLPKRLELHRRKLFALSIKPERLQQIRNERRP
                     ESRYSSRPQVQYEIRAAEALFVRYAIPCLDTTECSIEEIASRILNTTGIERRLRP"
     gene            5802..6215
                     /locus_tag="VFB37_02655"
     CDS             5802..6215
                     /locus_tag="VFB37_02655"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HZO21377.1"
                     /translation="MGLYESNYIRLGWLVSGLGTLTGRQRSVVPGDCDLLLTLSERSP
                     YTTTLNLTYVLPAASGEEPLPDMRVRVYHDAHLAEALEWAGSHSQPILRTLRSHAERE
                     LDQRWARNVMLNKWLEYCVERGHRFSSGASVTAGR"
     gene            6243..7301
                     /locus_tag="VFB37_02660"
     CDS             6243..7301
                     /locus_tag="VFB37_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21378.1"
                     /translation="MKALRQLGFLKLNFRQDAPPEEEGRVLALFRNRAELKKAYGELQ
                     EEIYRLQDRMKQQEGATQRVQEMLSALEGRLGMSDTAYPALVFYQLRRLWQSGRELIE
                     QFVADLTRQQEDRERRAHLAEHNRRQFTRRQSVEKTLRSAESQAAEMADSVAGLESER
                     ARLTRFWHYFKRRRLEHRIAAAVGAAVSANAALVEARAASEALEQEPVPEFAGLSLEA
                     RRAINLAAIAYAEVLCLRLVKTPLVNLAREATSRREVADEYGSQQECERLMDEIESAR
                     VAIQVRTNIAQEITSRSERLKQVARYRSAADTAPTTDSLAFSEGDVMGSDPVGASAAR
                     MPNVLAEDTWDLFRILLR"
     gene            complement(7346..9475)
                     /locus_tag="VFB37_02665"
     CDS             complement(7346..9475)
                     /locus_tag="VFB37_02665"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HZO21379.1"
                     /translation="MLHKSLRRTTGWMAASVLTTLAVLALALAGPSRAPASPSILPPG
                     ALAPTDAQRSTARKIGRILEEAHYSRMPIDKKLSEQVFQRYLDFLDSQHSYFLASDIA
                     EFQAYRFKFDDMIRTGDVDPAYVIFARFQQRNRERIQHAIELLKTEPDWTLNETFEFD
                     RTKTAWPSNENELNELWRKRVKNDALSLMLTGKQWPEVSDLLRKRYEHVLHRVDQITS
                     EDVFENLMNAYARSFDPHSSYFSPRNSEEYRIQMSLNYEGIGASLQLVDDYVTIMNVI
                     EGGPAAVAQTLNQNDRVTAVGQGHDGEMKDVIGWRLDDVVQLIRGKAGTIVRLEVLPA
                     GAAPGSKEKTLEFVRNKVTLEAQAAHKEVKNVTRNGRTLKVGVITVPGFYQDIAAQNA
                     GDQNYRSTTRDVLKLLKELKTENIDGLVLDLRGDGGGYLPEATALTGLFIDHGPVVQL
                     KDTTGRLEVLDDPEQAPAYDGPLAVLVDRFSASASEIFAGAIQDYHRGLILGQRTFGK
                     GTVQNLVPLDRWSQKPVSGQLTVTIGKFYRVTGESTQHRGVEPDLTLPSPIDTKEVGE
                     SALEAALPWDRIAGVPFQKSDPPGTLPVSALVSDEDIRAQRDPDYRWLVSDIAAIDSV
                     REQHSVSLNLKERREERARQDKERLDRENSRRAAKNLPPLKSVEDIEKDKNELPDVVL
                     SQATEVMADMVTGVRPSAPSQKTARRS"
     gene            9576..9863
                     /locus_tag="VFB37_02670"
     CDS             9576..9863
                     /locus_tag="VFB37_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21380.1"
                     /translation="MRPLTILLGIVMGSTVSLAVGLVMTWIVILFLPRDAERFAPEHA
                     PLLQAIAIFTALAAATAASFYGDLRTRTWRLGAHALTVLLLGATIWMYWPS"
     gene            complement(9974..10339)
                     /locus_tag="VFB37_02675"
     CDS             complement(9974..10339)
                     /locus_tag="VFB37_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21381.1"
                     /translation="MPRLASRSLLLLPVHRSFAHLGSLTRELLILGVCLLAGVTLVPL
                     LIWVVGNRALGPYTHGTNTHAGPMALLGDFLVGLSQGAFTYWLVALGPAILIVLLRIC
                     FALLLRRPEATPPSHPSPH"
     gene            10459..11424
                     /locus_tag="VFB37_02680"
     CDS             10459..11424
                     /locus_tag="VFB37_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZO21382.1"
                     /translation="MQQVRAAVVGVGYLGRFHAQKYAQAHDCKLVAVVDASPSAREAI
                     TTELGVRAIAQYQDLLGAVDAVSIVTPTPTHFPIARDFLAAGVHVLLEKPITETGTQA
                     RELIALAAAHKRILQVGHLERFNAAILAAEPYLRMPRFVECHRLAPYRERGTDVNVVL
                     DLMIHDIDIVQTMVGASIATIDAVGTPVFSEEIDICNARIRFANGCVANATASRVSLK
                     TERKLRVFEDDAYLSLDLQQKILTVIRKRSPGDPPGPLPVTIEEQALEQGDALKAEIE
                     SFLQCVRTGASPVVSGEAGLTALETATRITEQVHESLAARAGHRH"
     gene            11435..12361
                     /gene="aguB"
                     /locus_tag="VFB37_02685"
     CDS             11435..12361
                     /gene="aguB"
                     /locus_tag="VFB37_02685"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HZO21383.1"
                     /translation="MSLQPEHFRRPSRRVSVAAIQMSCGWDIQANIAKAESLVRQAAA
                     RGAQIILLPELFEAPYFCIEQDNRHLALARPVQESVPVQHFSGVARELGVVLPISFFE
                     RSGPVFFNSIAIIDADGVNLGLYRKSHIPNGPGYQEKNYFSPGDTGFRVWDTRFARIG
                     VGICWDQWFPETARSMALLGAELFFFPTAIGSEPPPALPVNSREHWQRTQQGHAAANL
                     TPLIACNRYGLERSIQDPDGLYIRFYGSSFIADATGAKLAEAPEEGDAVLTADFDLEE
                     LGQLRNNWFVFRDRRPDLYGSLVSLDGGSDRH"
     gene            complement(12419..13099)
                     /locus_tag="VFB37_02690"
     CDS             complement(12419..13099)
                     /locus_tag="VFB37_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HZO21384.1"
                     /translation="MKREDHEIEGRRHEPEDRGTEQGLTGARTLASTGELPEAVNQCR
                     RCSLWRTATQGVPGEGKSPNALMLVGEAPGDSEDLQGHPFIGPAGAMLDRALGEAGLD
                     RQSVYVTNAVKHFKFEPRGKRRLHVKPSPSEIEACNWWLVEELRLVGPKLVIALGATA
                     ARALLGQVVTISQMRGTPVSLSATTHLWVTIHPSLLLRIPDDVRRRAEFDRFVRELKD
                     ARQWVARH"
     gene            complement(13149..13499)
                     /gene="ssrA"
                     /locus_tag="VFB37_02695"
     tmRNA           complement(13149..13499)
                     /gene="ssrA"
                     /locus_tag="VFB37_02695"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13590..14057)
                     /gene="smpB"
                     /locus_tag="VFB37_02700"
     CDS             complement(13590..14057)
                     /gene="smpB"
                     /locus_tag="VFB37_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZO21385.1"
                     /translation="MAKAEQPQRLIAENRKARFDYFIEERYEAGIALQGWEVKAMRAG
                     RAQLKEAYVFVRGFEAFLFGAHIAPLNSASTHVVADPTRTRKLLLHRSELSGLIGAVE
                     RKGYTLVPLELYWKNGRAKLQVGLAKGKKQHDKRAVEKERDWQRDKARLLRRG"
     gene            14184..14621
                     /locus_tag="VFB37_02705"
     CDS             14184..14621
                     /locus_tag="VFB37_02705"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HZO21386.1"
                     /translation="MRQVQHSALIVQTPGRVFSLINDIESYPRFIPWCTHASVLSRSE
                     REIIATIGVRRGPLRGEFTTRNELEPDRRILMHLVSGPFRMLEGEWLLSPVGDNQAGC
                     RVQLTMKFAFSNPLTALLFEQQFAETAASLLDAFVARARTLPP"
     gene            14618..14923
                     /locus_tag="VFB37_02710"
     CDS             14618..14923
                     /locus_tag="VFB37_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HZO21387.1"
                     /translation="MTRPVRKQCTVAYATSEQQYLWSVDLPWEANIADALEAARTIAS
                     SAQVPWESAAVGIFGERRQRSEIPAPGDRIELYRPLRCDPRQRRREQVQKQRKGTPR"
     gene            complement(14978..15352)
                     /locus_tag="VFB37_02715"
     CDS             complement(14978..15352)
                     /locus_tag="VFB37_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZO21388.1"
                     /translation="MKPTFFRLGVLLLSLLCLQACVYRMNIQQGNYLEARTVDQLQVG
                     MTRSQVRYLLGTPMVPDIFDKGRWDYLYYFKKGRLHRAEERHLIVLFHEDKVEKFEKI
                     NVPNKAPQGPEQGPSIQKFPVM"
     gene            15454..15888
                     /gene="fur"
                     /locus_tag="VFB37_02720"
     CDS             15454..15888
                     /gene="fur"
                     /locus_tag="VFB37_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HZO21389.1"
                     /translation="MEGKDLRKAGLKVTLPRLKILEILESSATRHLSAEDIYRNLLDS
                     NEDIGLATVYRVLTQFEAAGLVTRHHFEDGMAVFELNQGTHHDHIVCLDCGRVEEFMD
                     AGIEDRQTSVAQRLGFTIRDHSLIMYGHCRRTNCAYRKKKAS"
     gene            complement(15903..>16274)
                     /locus_tag="VFB37_02725"
     CDS             complement(15903..>16274)
                     /locus_tag="VFB37_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21390.1"
                     /translation="LPQVASQGHHHLRVTKLTDGRTSRTALAELAPQERVEELARMLG
                     GVEVTARAREHAREMLKAISPPAADPGATGRVNIPAGEAPREAASAAARGAPVRSGAP
                     APIAPERTEEEEAARPASRRR"
BASE COUNT         2859 a         5309 c         5186 g         2920 t
ORIGIN      
        1 cggctccgcg ccacctcgag cgttggatcg atacagcgct ccgtgccttg ggtgcacatc
       61 gaacacgtgg atgtcggcgc tgcgcggatc gatgcggcac gagacgtggt cgccgcagac
      121 caccacgtgg ccccgcccga aaggcctggc tttcaattcc cgtcccgcgc gatcccgcac
      181 gagcaaatgg cgcccgtaag tggcggtgac gagggcctcg aaatgctcac tcatgagaac
      241 cgcagcgtaa cctctggtaa aatcaggctc aatccatatc gaccctgcgc atgccaagcg
      301 gcgaaaacct catctggatc gatctcgaaa tgaccgggct caggccggac gtcgactcga
      361 tcatcgagat cgcgaccgtc gtcacggata aggaactgac ggtgatcgcc gatggcccgg
      421 tgctggccat tcatcagccg caggaagtcc tcgatcgcat ggatgagtgg aatcagaagc
      481 agcatggctc ctcgggactc actgcacgcg tgcgctccag tcgcatcagt gaggcggaag
      541 ccgaagagcg cacgctcgag ttcctggcac ccctggtcac gggcggcgca tctcccatgt
      601 gcggaaacag catttgccag gatcggcgct ttctcgcccg gcacatgccg cggctcgagc
      661 gctacttcca ttaccgcaac ctcgatgtca gtaccctcaa ggagctcgcc agacgctggg
      721 cgcctgcggt agcggcgagt ttcctcaagc aggggacgca cctggcccat gccgacatcc
      781 aggagtccat ccgcgagctt cgtcattacc gtacgcacct gttggccccc gcctacgctg
      841 cccggcagtg agtaccgaaa caacatcgct cccgagcttt gcggatgtcc tggccgccgc
      901 tgaccgcatc ggctcgcgcc tcgtgcgaac ccctgtcatg agttcgccca ggctcgatga
      961 gcgggccggg tgccgcgtcg tgttcaaatg cgaaaacctc caacacggtg gagcgttcaa
     1021 gtatcgcggc gccctcaatg ccattctgcc gcttgcggtg gccggaaacg tcgccgcggt
     1081 tgccacgcac tcctccggaa atcacggcac ggcggtggcg ttggcagcac gggtctgcgg
     1141 cctcgatgct catgtggtgg tgccgcgcga ctcggctcgc cccaagatcg cggcaatcga
     1201 gcgggccggc ggacacgtga ttctctgtga gccgggtcag cccgcgcgcg aagcggctct
     1261 tgccgatctg ctgcgcgaag tcgatgccca tgtggtgccc cccttcgatg atgcgcgggt
     1321 catcgccggc caaggcaccg ccgcgctgga gcttttcagt gaggagcccg gcctggacct
     1381 cgtcagcacc ccgatcggcg gcggcggcct catcgggggc acggcactcg ccgcgcaggc
     1441 ccttgcgccg cgctgccggg tcgtcggtgc ggagcctgcg ggggcggacg atgcgtatcg
     1501 ctctttccgc tccggaaccc gccagccggt cgtgaagccg caaacgattg cggacgggct
     1561 gcgaggatcc atcggcgcgt tgaacttcga gctgctgcgc cgtcatgtcg ccgacgtcgt
     1621 caccgtctcg gagcgggaga tagtcgaggc gatgcgcatc gttctgcagg acctgaagct
     1681 tctgatcgag ccttcctcgg ccgtgccggt cgccgcgctg ctggcgggaa agctcggcag
     1741 tgccggtcaa cgagtcggca tcatcctgtc gggcggcaac gtcgatctcg agcagtgccc
     1801 gtttctgcgc ggtgagcggc tcgcttgacg gaggagctgc tcacccaccg tggcggctgt
     1861 cattgtggcg cagtagcctt cgaggtgctg gcgccagcgc gcctgcgtgc cagcgattgc
     1921 aactgctcga tctgccggat gagcggctat ctgcacctca tcgtgccgca gggcagattc
     1981 cgcctgctcc gcggagcgga gctgctcacc gagtatcgat tcaacaccgg tacggcgcgg
     2041 catctgttct gcgcgcgctg tggcgtgaag tcattttacg taccgagatc gaacccggac
     2101 ggctacagca tcaacgtgcg ctgcctcgat ccctcgacag tggcacacgt ggaggtggaa
     2161 ccgttcgacg atgacgatcg ggaggcggcc accgcgagga tccgggagct ctcgcgccaa
     2221 ggctgaaggc cgccggatct ctcctgctca acctttcgcc ggctttccgg cgctctccgc
     2281 caggaacatc cacgtctcga ccaccgtatc cgggttgagc gacatgctct cgatcccctg
     2341 ctcgagcagc cagcgcgcca gctccggatg atccgagggt ccctggccac agatgcccac
     2401 gtacttgccg cgcctcttgc aggcggcaat cgtcatggcc agcagcattt tcaccgcctc
     2461 gtcccgctca tcgaaagcct tcgccacgat cgaggagtcg cgatccagac cgagggtgag
     2521 ctgagtcatg tcgttcgagc cgatcgacat gccatcgaaa tgattcagat actcgtccgc
     2581 gagcagcgcg ttggtgggca gctcgcacat catgatgatc cgcaggtcgc ccgtgccgcg
     2641 ctcgagaccg ttctcggcga gcagctcggt gacctgcctg gcctccttca gcgtgcgcac
     2701 gaagggcacc atgacctgca cgttggtgag gcccatctcg tcgcggacct tcttcagagc
     2761 gagacattcg agctcgaagc acggccggaa ggtctcatcg acgtaacgcg aggcgccacg
     2821 aaagccgagc atcggatttt cttcttccgg ctcgtagcgc gcgcccccga tgagattcgc
     2881 atattcgttc gacttgaaat cggacagccg cacgatcacc ggctccggcg caaacgccgc
     2941 ggcgatctgc gcgatgccct cggcaagctt gtccacgtaa aacccgaccg gatcggcgta
     3001 gccggccatc tggccgcgga tttgctcctg cagctcgctg tccagctgat cgaactccag
     3061 cagcgcgcgc gggtgcacgc cgatcatccg gttgatgatg aactccagcc gcgcaagtcc
     3121 tactccacgg tgagggatgc ctgcgaaatc gaaggcacgg tccggattgc cgacgttcat
     3181 catgatcttg accggaatct tcggcagcgt atcgagctcg atctgcttgc gctcaaactc
     3241 cagcatgccc tcgtacacgt agccggtgtc tccctcggcg caggataccg tgacgtcctg
     3301 tccctcacgg atgctctgcg tcgcatcggt acagccgacc acggctgcaa ttcccagctc
     3361 acgtgcaatg atcgccgcgt gacaggtccg gccgccgcga ttggtgacga tcgccgaagc
     3421 gcgtttcatg acgggctccc agtcggggtc ggtcatgtcg gccaccagca cgtcaccatt
     3481 ctggacacgg gccatctcgg aggcatcctt gatcacacgc gccggaccgg ctccgatgcg
     3541 ctgaccgata ctgcgcccgg aagtgagcac cctggagcgc gacttgagcg agaaacgctg
     3601 gatggtgcga cccgcgcggc tctgcacggt ttccggccgc gcctgcagaa tgaagatctc
     3661 acccgaggcg ccatccttgc cccactcgat atccatcggc gccttgtaat ggtcctcgat
     3721 gagcagcgcc tggcgcgcca gcgccagaag atcggcttcc tccagacaga agcgcatgcg
     3781 ctcgtgctgc ggaacctcca cggttctcac gcgctcggat gagccctcgg gcgcgtacac
     3841 catcttcacg gccttgccgc ccaggttctt gcgaaggatc ggatatttcc ctgcgcgcaa
     3901 tcccagcttg tagacgtaga actcatcggg attcacggct ccctgcacca cggtctcgcc
     3961 caaaccgtag gaggcagtga tgaacacgac atcgcgaaag ccggaatcgg tgtcgagtgt
     4021 gaacatgaca ccgctggagc cgagatcact gcgtaccatg tgttgcacac cggcggaaag
     4081 tgcgacggcg ttatggtcga agccctgatg cacgcgatag gagatcgcgc ggtcattgaa
     4141 cagcgaggcg aaaacctcgt gcatggctcg cagcacattc gcttcgcccc gcacgttcag
     4201 gaacgtctcc tgctggccag cgaaagaggc ttcgggcaga tcctcggcag tggccgagga
     4261 tcgcactgca accgcaattt cgctgccgcc gctcatgcgg cgcagctcct ccgcgaccgc
     4321 cttctgcagc tccgggtgaa acggcgtagc catgatccag ccgcggatgc gcgcgccggt
     4381 cgctgcaagt cgcgtgacat catcgacgtt caacgaggcg agctcggcgc gaatccgtcc
     4441 ctcgagatca ccctgcttga ggaaatcccg atacgcctgc gcagtagtcg cgaagccgcc
     4501 gggtacacgc acgccgagac tggccaggga gccgatcatc tcgcccaggg aagcattctt
     4561 gccgccgaca gtctccacgt cacgcatggc aaggcgctcg aaaggaatga catacctgtt
     4621 caatggcgct cctcggcaga cgtgcgaacg tgaatcatgc ttgctccggc tgccgggcgg
     4681 tggaacactc gctgcacgct gggcaaccgg acaggctcgt gagtcgtcga accgtattct
     4741 tcgtttcgga ccagacgggt gtgactgcgg aaaccatggg ccacagtctc atgacgcagt
     4801 tcgaaggcct ggaatttcga cccgtgactt tgccatttgt gtcgagcgtt gacaaggcgc
     4861 atgaggcggt cagacgcatc aaccgcgccg cggaagaaga ggggctgcgg ccgatcattt
     4921 tcagcactct ggtgcaggac gagctgcgcg acatcgtagt ctcggccaac ggtttgttcc
     4981 tggacttctt cgccgcgttc gtgggtccgc tcgagcgcga gctcaacact cgctccaccc
     5041 accgcgccgg ccgcgcgcat ggcatggcgg acgttgccgc ctataccgcc cgtatcaacg
     5101 cgacgaactt cgcgcttgcc aacgacgacg gcactggtgg cgactacgcg cacgccgatg
     5161 tgatcctcct cggcgtgtcc cgctccggca agacgccgac gtgcctctac atggcgctgc
     5221 agtacggtgt gttcgccgcc aactatccgc tcaccgaaga ggatctcgag gcgggacgct
     5281 tgcccaagcg gctcgagctg catcgacgca agctgtttgc cctgtcgatc aagcccgagc
     5341 gattgcaaca aatacgcaac gagcgccgcc cggagagtcg ctactcgtcg cggccgcaag
     5401 tgcagtacga gatccgcgcg gccgaggcat tgttcgtgcg ctacgcgatt ccctgtctgg
     5461 ataccaccga gtgctccatc gaggagattg ccagtcgtat cctgaacacg accggcatcg
     5521 agaggcgtct gcggccgtag agaaattccg gcaattatcc cgcgcgagtg ctgtcggtgg
     5581 cacgattcgt gcgtgcgttg actggcaact gcggagcgct ctgaaaatcg cgcatttcat
     5641 gtcttgcacg cgccccgagc tgcgttatag tcaggaggat gctgaaacca gccaccagaa
     5701 ccagacctgc acgagcggcg cgcgcaagcg ctcgcgccgc tttcgatgac acgctggctc
     5761 cggcttcctg gcgcgcgagc ccgcgcactt ttgtcgcgct catgggtttg tacgaaagta
     5821 actacattcg actcggctgg ctggtgagcg gactcggaac actcacggga cggcagcgct
     5881 ccgtggtgcc cggagactgt gatcttcttc tcacgctgtc ggaacgctcc ccgtacacga
     5941 cgaccctgaa tttgacgtat gtactgcccg cagcctctgg cgaggagccg ctgcccgaca
     6001 tgcgcgtacg ggtctatcat gacgcgcatc tcgccgaagc tctcgagtgg gccggcagcc
     6061 acagccaacc cattctcagg acgctgcgca gccatgcaga gcgtgaactg gatcagcgct
     6121 gggcgcgcaa cgtgatgcta aacaagtggc tggaatattg cgtggagcga ggtcatcgct
     6181 tctcatccgg agcgagcgta acggccggta gatgagaagc ggcggttccc gccgcagctc
     6241 tcatgaaggc ccttcgacaa ctcggatttc tcaagctcaa cttccgtcag gatgcgcctc
     6301 ccgaagagga ggggcgcgtg ctggcgctgt ttcgcaatcg cgccgagctc aagaaggctt
     6361 acggcgagct gcaggaggaa atctatcgcc tgcaggaccg catgaaacag caggaagggg
     6421 ccacacagcg cgtgcaggaa atgctcagcg cgctggaggg tcgcctcggc atgtccgata
     6481 cggcctatcc cgccctcgtt ttctatcagc tacgcaggct gtggcaaagc ggacgcgagc
     6541 tgatcgagca gttcgtggcg gatctgaccc ggcagcagga ggatcgcgag cgccgggcgc
     6601 acctcgccga gcacaaccgc cgtcagttca cccggcgcca gtccgtggag aagacgctgc
     6661 gcagcgccga gtcgcaggcg gctgagatgg ccgacagcgt cgccggtctc gaaagcgagc
     6721 gtgcgaggct cacccgtttc tggcactact tcaagcggcg caggctcgag caccggatcg
     6781 ccgccgcagt cggcgccgcc gtcagtgcca acgccgctct ggtcgaagcg cgcgcagcct
     6841 ccgaggctct cgagcaggag cctgttcccg agtttgcggg cctgtcgctg gaagcgcgcc
     6901 gggccatcaa tctcgcagcc atcgcctatg ccgaagtgct gtgcctgcgg ctggtgaaga
     6961 cgccgctggt gaatctggcg cgcgaggcga ccagccggcg cgaagtggcc gacgagtatg
     7021 gctctcagca ggaatgcgag cggctgatgg atgagatcga gagtgcgcgt gtggcgattc
     7081 aggttcggac gaacattgct caggagatca ccagtcggag cgagagactc aagcaggtcg
     7141 cacgctacag aagcgctgcc gacacagctc cgacgacgga ctctctcgct ttctccgagg
     7201 gcgatgtgat gggcagcgat cccgtcgggg caagcgcggc gcgcatgccc aacgtgctcg
     7261 ccgaagacac ctgggatctg ttcagaatcc tgctgagatg aggatacccg ggccggctcc
     7321 ccgccggccc gggctcaaaa gggcttcagc ttcggcgcgc ggtcttctgg gacggtgccg
     7381 aggggcgtac accggtcacc atgtccgcca tcacctctgt cgcctgagaa agcacgacgt
     7441 ctggtaactc gttcttgtcc ttctcgatgt cctccacgga cttcaggggc ggcaggttct
     7501 tggcagcacg gcggctgttc tcgcgatcga gtcgctcctt gtcctgccgg gcgcgctcct
     7561 cgcggcgctc cttcaggttc agcgaaacgg agtgctgttc acgcacggag tcgatcgctg
     7621 caatgtcgga gacgagccag cggtagtcag gatcgcgctg cgcgcgaatg tcctcgtcgg
     7681 acaccagcgc cgagacgggc agggtgcccg gcggatcgct cttctgaaag ggcacgcccg
     7741 caatccggtc ccagggcagc gcggcctcga gtgcgctctc gcccacttcc ttggtatcga
     7801 tcggtgaggg caatgtcagg tcgggctcga cgccccgatg ctgggtactc tcaccggtca
     7861 cgcggtagaa cttaccgatg gtgacggtga gctggccgct caccggcttc tgcgaccacc
     7921 gatccagcgg caccagattc tgcacggtgc ccttgccgaa ggtgcgttgc cccagaatga
     7981 gcccacggtg atagtcctgg atggcgcctg cgaagatctc ggaggcggag gcactgaagc
     8041 gatccacgag taccgccagc gggccgtcgt aggctggagc ctgctccgga tcatcgagca
     8101 cctcgagccg cccggtggtg tccttgagct gcaccaccgg cccgtgatcg atgaacagcc
     8161 cggtgagcgc agtggcttcc ggcagatatc cgccgccatc cccgcgcagg tcgagcacca
     8221 ggccgtcgat attctcggtc ttcagctcct tcagcagctt cagcacatcg cgcgtcgtgc
     8281 tgcggtagtt ctgatcgccc gcattctgtg cggcgatgtc ctgatagaag cccggcacgg
     8341 tgatgacacc gaccttgagc gtgcgcccgt tgcgagtgac gttcttcact tccttgtgtg
     8401 ccgcctgggc ctccagggtg accttgttgc gcacgaattc gagtgtcttt tccttcgagc
     8461 ccggcgcggc gcccgcgggc aggacttcca ggcgcacgat cgtgccggcc ttgccgcgga
     8521 tgagctgcac cacgtcatcg agccgccagc cgatcacatc cttcatctcc ccatcgtgac
     8581 cctgacccac tgccgtgacg cgatcgttct gattcaacgt ctgggcaacg gccgccgggc
     8641 cgccctcgat cacgttcatg atcgtcacgt agtcatccac gagttgcagc gaggcgccga
     8701 tgccctcgta gttgaggctc atctggatgc gatattcctc ggaattgcgc ggcgagaaat
     8761 agctcgagtg cggatcgaac gagcgcgcat acgcgttcat caggttctcg aacacatctt
     8821 cggaagtgat ctgatcgacg cggtgcagga cgtgctcata gcgcttgcgc agcaggtcgg
     8881 aaacctccgg ccactgcttc ccggtgagca tcagcgaaag ggcatcgttc ttcacccgct
     8941 tgcgccacag ctcgttcagc tcattctcgt tcgacggcca agcggtcttg gtgcgatcga
     9001 actcgaatgt ctcgttgagc gtccagtcgg gctcggtctt gagcagctcg atcgcatgct
     9061 gaatgcgctc gcggttgcgc tgttgaaagc gcgcaaagat cacgtaggcg ggatcgacat
     9121 ccccggtgcg gatcatgtca tcgaacttga agcggtaggc ctggaactcc gcgatgtcgc
     9181 tcgccaggaa gtagctgtgc tggctgtcga ggaaatccag gtaacgctgg aagacctgct
     9241 ccgacagctt cttgtcgatc ggcatccggc tgtagtgcgc ctcctcgagg atgcgaccga
     9301 tcttgcgggc agtggaccgc tgcgcgtctg tcggggcgag ggccccgggg ggcagaatgg
     9361 aagggctggc cggtgcacgc gagggacccg cgagggcgag tgccaggacg gccagggttg
     9421 tcagcactga ggcggccatc cacccggtcg tgcgccgaag agatttatgc agcattcatg
     9481 acgctcgcag tttcgtaagg cttacacttt ggagcaaggc tagagcgtgc ggttcggctc
     9541 agcaagtgcg ggaactctcc agtctggtgg acagcatgcg tcctctcacc atattgctcg
     9601 gaatcgtgat gggctcgacg gtctcgctgg ccgttggact agtaatgacc tggatcgtca
     9661 tcctgttcct gccccgggac gccgagcgat tcgccccgga acacgccccg ctcctgcaag
     9721 ccatcgcgat cttcacggcc ctggcggccg ccacggccgc cagcttctat ggcgacctgc
     9781 gcacccgcac ctggcgcctg ggggcgcacg ccctcacggt gctgctgctg ggggcaacca
     9841 tttggatgta ctggccatct tgatggcccg gcacccagct cggacttggc gggcgatcta
     9901 gagcgttcca tgcgaaccac tgatcggatt cgagcctcgc tgccggaagg aggttccgtc
     9961 gcgccggtat tacttaatgg ggagaggggt gggaaggcgg cgttgcctcg ggccgccgca
    10021 gcagcagcgc gaagcatatc cgcagcagca cgatcaggat ggcggggccg agagcgacca
    10081 gccagtacgt gaaggcaccc tggctgagtc ccaccaggaa gtctccgagc agggccatgg
    10141 gaccggcatg cgtgtttgtg ccatgcgtgt acggccccag agcgcgatta ccgactaccc
    10201 agatcaggag tggcacgagg gtgacgccgg ccagcaggca cacgcccagg atgagcagct
    10261 cgcgggtcag cgagcccaga tgagcaaagg atcggtggac gggcagcagc agaagactcc
    10321 ttgaggccag ccggggcacg gcctaatctt cgaaaggccg ccgagcgccc cgccgcctca
    10381 aacgaccggc cgggcgacct gtgtccttcg cggtgcgccc caaggcgagc cctcgcaggc
    10441 tggagagtat acgcgtcgat gcagcaagtc agagcagcag tcgtcggtgt cggctatctg
    10501 ggccgattcc acgcccagaa atatgcccag gcgcacgact gcaaactcgt tgcggtggtg
    10561 gacgccagcc cgtccgcaag ggaggcgatc acaacggagc tgggtgttcg cgcgatagcg
    10621 caataccagg atctgctcgg cgcggtcgac gctgtgagca tcgtcacgcc tacgcccaca
    10681 cactttccca tcgcgcgtga ctttctcgct gccggggtcc acgttctgct cgaaaagccg
    10741 atcaccgaga ccggaacaca ggcgcgcgag ctcattgcgc tggccgcggc ccacaagcgc
    10801 attctccagg tgggtcacct cgagcgcttc aacgccgcga ttcttgctgc cgagccatac
    10861 ttgcgcatgc cacggttcgt cgaatgccac cggcttgctc cctatcgaga gcgtggcacg
    10921 gatgtcaatg tcgtgctgga tctgatgatt catgacatcg acatcgtgca gaccatggtg
    10981 ggtgcttcca ttgcgaccat cgatgcggtc ggaacgcctg tgttctcgga agagatcgac
    11041 atctgcaacg cgcggatcag atttgccaat ggttgcgttg cgaatgcgac cgcaagccgg
    11101 gtgagcctga agaccgagcg caagctgcgc gtgttcgagg atgacgctta cctgtctctg
    11161 gatctgcagc agaagatcct cacggtgatc cgcaagcgca gccccgggga cccgccgggc
    11221 ccgctgccgg tcacgatcga ggagcaggcg ctggagcagg gggatgcgct caaggccgag
    11281 atcgagtctt tcctgcagtg cgtgcgcact ggcgcctcgc ccgtagtgag cggggaagcc
    11341 gggctgaccg cgctcgagac ggcgacgcgc atcaccgagc aggtccatga atcgctcgcc
    11401 gcgcgcgccg gccaccgtca ctgagaggtc cgcgatgtcc ctgcaaccag agcacttccg
    11461 ccgtccgagc cgccgcgtgt cggtggcggc catccagatg tcctgcggct gggatattca
    11521 ggccaacatc gccaaggcgg agagcctggt gcgacaagcc gccgcccgcg gcgcgcagat
    11581 catcctgctg ccggagctgt tcgaggcacc ctatttctgc atcgagcagg acaatcgaca
    11641 cctcgccctg gcccgtccgg tgcaggagag cgtgcccgta cagcacttca gcggcgttgc
    11701 ccgcgagctc ggggtcgtcc tgccgatcag ctttttcgag cgctccggcc cggtattctt
    11761 caactcgatc gccattatcg atgccgatgg ggtgaacctc ggtctctacc gcaagtcaca
    11821 cattcccaac ggcccggggt atcaggagaa gaattacttc agtccgggcg acaccggctt
    11881 cagggtctgg gacacgcgct tcgcacgcat cggggtgggc atctgctggg accagtggtt
    11941 tccggagacg gcccgctcga tggcgctgct cggtgccgag ctgtttttct ttccgaccgc
    12001 catcggctcg gagccgcccc cggccttgcc ggtgaattcc cgcgagcact ggcagcgcac
    12061 ccaacagggc cacgctgcgg ccaacctgac tccgctcatc gcctgcaacc gctacggcct
    12121 ggagcgctcg atccaggacc ccgatgggct gtacatccgc ttctacggct cgtccttcat
    12181 cgcggatgca acgggtgcca agcttgcgga ggccccggag gagggtgacg cggtgctgac
    12241 ggccgatttc gatctggaag agctcgggca gctgcgcaac aactggttcg tcttccgcga
    12301 ccgccgtccc gacctctacg gttcgctggt gtcactcgat ggagggtctg accggcactg
    12361 aacaaccggc gctgtgtagg tcgccattga gcgggcccga gctgagcggc gccggcgctc
    12421 agtgcctcgc gacccactgg cgcgcatcct tcagctcgcg cacgaagcga tcgaattcgg
    12481 cgcgcctgcg gacatcatcc gggatgcgca gcaggagcga agggtggatc gtcacccaca
    12541 agtgtgtggt ggcgctcagg ctcacgggcg tgccacgcat ctgcgagatg gtcacgacct
    12601 ggcccaacag ggcacgtgcg gccgtcgcgc ccaacgcgat caccagcttc ggcccgacga
    12661 ggcgcaattc ctcgaccaac caccagttgc aagcctcgat ctcgctcggg ctgggcttga
    12721 catgcagacg gcgtttgccg cgcggctcga acttgaaatg cttgaccgca ttcgtgacat
    12781 aaacggactg gcggtcgagg ccggcctcac cgagcgcccg gtcgagcatc gcaccggcag
    12841 ggccaatgaa ggggtgtccc tgcaggtcct cggagtcccc gggcgcctca cccaccagca
    12901 tcagcgcgtt gggtgacttg ccttccccgg gcacgccctg agtcgccgtc cgccacagtg
    12961 agcaccgtct gcactgattg acagcttccg gcagctcgcc ggtggaggcg agagtgcggg
    13021 cgcccgtgag cccttgctcc gtgccacgat cctcaggctc gtgacgcctg ccctcgatct
    13081 catggtcttc gcgcttcatc cgacagcttc aatgatcgaa gccgcccggt ggttccgggg
    13141 aaagagtttg gtggaggcgg ggggaatcga acccccgtcc gcaagcgcta gactccaagc
    13201 tctacgtatg tagctcgctc tttgtttctc gcgtcacgct acccgaggag cagggaagac
    13261 gcttcgccag ccacggtgac tcttaacgac acggcccgtc gcacaccgca tcgcgatcct
    13321 atgagcgcgt cccctgattc agtcgcatag gcacgagctg gtcagaggtc agctgcgttt
    13381 aggcagctag agcgtagttg tcgtcgttgg caactatgat ttgcagcatt attaacgagg
    13441 gcactgcgcc tcgatacgca cctggaggtt tgcaacccac gtcgaaaccg gtcgccccct
    13501 aaggtcctgc ctagcataac ccaggagccg cactcccggg ggaaatgccc ccgcgggcct
    13561 gcataagccc cggtccgcag gtcccggggt cagcctcgcc tgagcagccg cgccttgtcg
    13621 cgctgccagt cgcgttcctt ctcgacggcc cgcttgtcat gttgcttctt gcccttggcg
    13681 agccccacct gcagtttcgc gcggccgttc ttccagtaca gctcgagcgg caccagggta
    13741 tagcctttgc gctccaccgc gccgatcagt cctgagagct cgctgcggtg aagtagcagc
    13801 ttgcgagtgc gggtcgggtc cgccaccacg tgggtggatg ctgagttgag tggggcgatg
    13861 tgcgcgccaa acaggaacgc ctcgaagccg cggacgaaga cgtacgcctc tttcaactgc
    13921 gcgcgtccgg cgcgcattgc cttcacttcc catccctgca gggcgatgcc ggcctcgtag
    13981 cgctcctcga tgaagtagtc gaagcgcgcc ttgcggttct cggcaatgag gcgttgaggc
    14041 tgttcggctt tggccatgct cgaagcaagg gtgaggggca gggcggcagt gtaccccagg
    14101 gcggctcgcg agcccggcgc agcctgtgcg gcccagcggc agccgatcat gacaaatcgg
    14161 gacgaccttg caacaatcaa cccatgcgac aggtccagca ctcggcgctc atcgttcaga
    14221 cgccgggccg cgtgttttcc ctcatcaatg acatcgaaag ctatcctcgc ttcatcccgt
    14281 ggtgtacgca cgcgagtgtg ctgtcccgtt cggagcgcga gatcatcgcc acgatcggcg
    14341 tgcgacgcgg cccgctgcgc ggcgaattca ccacacgcaa cgagctcgag cccgatcgcc
    14401 gcatcctgat gcacctggtg agcgggccgt tccggatgtt ggaaggagag tggctgctga
    14461 gccccgtagg ggacaaccaa gcgggctgcc gtgtgcagct caccatgaag ttcgctttca
    14521 gcaacccgct gacggcgctc ctgttcgagc agcagttcgc cgagaccgcc gcctcgctgc
    14581 tggacgcctt cgtggcacgg gcgcgaacgc tgccgccgtg acccgcccgg tgcgaaagca
    14641 gtgcacggtc gcctacgcca cctccgagca gcagtacctg tggagcgtcg atcttccctg
    14701 ggaggcaaac atcgcggacg ccctggaggc tgcgcgtacg atcgcgagct cagcacaggt
    14761 tccctgggag agcgcggccg tcggcatctt cggggagcgc cggcagcgct ctgaaatccc
    14821 ggctccgggg gatcgaatcg agttgtatcg gccgctgcgg tgcgatccac gccagcgccg
    14881 gcgcgaacag gtgcagaagc agcgcaaagg cacgccgcgt tagggaaccc gacgggctgc
    14941 gaaggcgggg cagcgtgttg gccgcggctc agccgctcta catcaccggg aacttctgga
    15001 tggagggtcc ctgctcgggg ccttgcggcg ccttgttcgg cacgttgatc ttctcgaatt
    15061 tctcgacctt gtcctcgtga aacagcacga tcagatggcg ctcttcggcc cgatgcagcc
    15121 ggcctttctt gaagtagtac aggtagtccc agcgaccctt gtcgaagatg tccgggacca
    15181 tcggggtgcc gaggaggtag cgcacctggc tgcgcgtcat gccgacctga agttggtcca
    15241 cggtgcgggc ttccaggtag ttcccctgct ggatgttcat gcgatagacg cacgcctgca
    15301 ggcaaagcag tgacagcagc aggacgccga gccgaaaaaa agtgggtttc atgccgttcc
    15361 aatgagggat aatggcggcg catcataccc gatcgccaac cgtccgccgc ccgcgccgcg
    15421 actgtggggc tggtccggac ctgaagagca ccagtggaag gcaaggacct ccgcaaagcc
    15481 ggcctgaagg taacgctgcc gaggctgaaa attctcgaga tcctcgagag cagtgcgacg
    15541 cgtcaccttt ccgccgagga catctaccgc aacctgctcg actcgaacga ggacatcggg
    15601 ctggcgacgg tgtatcgcgt gctgacacag ttcgaagctg cgggcctcgt cacccggcac
    15661 cacttcgagg acggcatggc cgtcttcgag ctcaatcagg gcacgcatca cgatcatatc
    15721 gtgtgtctgg actgcggccg cgtcgaggag ttcatggacg ccggcatcga ggatcggcag
    15781 acctcggtgg cgcagcgcct gggattcacc attcgcgatc attccctgat catgtacggc
    15841 cactgccgcc gcaccaactg cgcctatcgc aagaagaagg cgagctgagt cgttgcgggc
    15901 gatcagcgcc gcctggaggc tgggcgggcg gcctcttctt cctcggtccg ctccggagca
    15961 atgggcgctg gtgcgccaga acggaccggc gcgccgcggg ccgccgccga ggcggcttcc
    16021 ctgggcgcct cgccagcggg gatgttcacc cggccggtcg cgcccggatc cgcagcgggc
    16081 ggggaaatcg ctttgagcat ctcgcgggca tgctcacggg cgcgggccgt gacctcgacg
    16141 ccgccgagca tgcgtgccag ctcctccacg cgctcctgcg gagcgagttc ggcgagcgcg
    16201 gtgcgactcg tgcggccatc cgtgagtttc gtgacgcgca ggtgatgatg gccctgggat
    16261 gcgacctgcg gcag
//
LOCUS       DASRHN010000070         5709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_689871, whole genome shotgun sequence.
ACCESSION   DASRHN010000070 DASRHN010000000
VERSION     DASRHN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689871"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..570
                     /locus_tag="VFB37_02730"
     CDS             271..570
                     /locus_tag="VFB37_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21391.1"
                     /translation="MSEIADTFDSAFTKAVDLGNRLADKDKDADLWDIADGLLAGALQ
                     YWLYSRQPCGDPRCQDCMPISTAEGRMAELKRLMEQLASESEYFHTPTDANAGRA"
     gene            complement(582..1886)
                     /gene="gabT"
                     /locus_tag="VFB37_02735"
     CDS             complement(582..1886)
                     /gene="gabT"
                     /locus_tag="VFB37_02735"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZO21392.1"
                     /translation="MTSNAELHERRKNAIPRGVANNLAIYADRASNAELWDVEGKRYI
                     DFGTGIAVLNTGHVHPRIKGAIARQLDRFIHTCFQVTPYESYVALAEQLNALAPGKTS
                     KKTIFLSTGAEAVENAIKIARFHTRRSAVIAFSGGFHGRTLACIALTGKVQPYKAGFG
                     PMMPEVFHLPFPMTYHGITVEQSLKALDQLFKADVDPARVAAIIIEPVLGEGGFYVAP
                     PELLRKLRSICDEHGIVLIIDEIQTGFARTGRLFAIEHAGVEPDLMTVAKSIAGGVPL
                     AAVIGKADIMDAPMPGGLGGTYAGAPLACAAGLEVLEVIREEQLLNRAQHIGHFMTSR
                     LKGLQVRFPAIGDVRSLGAMVAVELVKNCSAEQPDADLAKALVQAASRRGLILLSCGV
                     YGNVIRFLAPLTIPDALLKEGFNLFEQALDEVTRAAVAAVPA"
     gene            2063..3100
                     /locus_tag="VFB37_02740"
     CDS             2063..3100
                     /locus_tag="VFB37_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO21393.1"
                     /translation="MSENPSITAEVVIIGAGPCGLFQVFELGLLGIGAHIVDSLQHPG
                     GQCAELYPEKPIYDIPALPVCGAQELVDRLMKQIEPFHATFHLGQEVVEFARQENGRF
                     HVRTTAGTTFDAGAVVIAAGVGSFQPRRIGVEGAEPFEGVNLHYRVKSSSAFHGQHLV
                     IFGGGDSALDWTLELLPKAASLTLVHRRPEFRAAPASVAKMQEHVSAGRMRLFEALPH
                     SLILDGGALRGVNIKARDGAMHALSADQLLVFFGLHPKLGAIAEWGLELEKKALKVDT
                     EKFQTSLPGVFAVGDINTYPGKKKLILSGFHEAALAAFAIQHHLYPQKKQFLQYTTTS
                     PIMHQRLGVPD"
     gene            3129..3605
                     /gene="azu"
                     /locus_tag="VFB37_02745"
     CDS             3129..3605
                     /gene="azu"
                     /locus_tag="VFB37_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HZO21394.1"
                     /translation="MKLIPNTLAALVVLASPALLLMSAPAAAAGDACKVEITGNDQMQ
                     YDKKEIDVPASCKEVTVTLHHSGQLPAAAMGHNWVLVNTPDLTAVANAGMGAGLASDY
                     VPAGDKRVLAHTKVVGGGQTTSVTFSTAALKAGGDYSYLCTFPGHNALMRGKFKFG"
     gene            3613..4476
                     /locus_tag="VFB37_02750"
     CDS             3613..4476
                     /locus_tag="VFB37_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HZO21395.1"
                     /translation="MDPCIADLLALLELEQLEVNLFRGESRDIGSPQVFGGQVLGQAL
                     TAASATVEGRVVHSLHAYFLRRGDFNAPIVYQVDRSLDGHSFSNRRVVAIQHGQQIFN
                     MAASFQVPEDGFDHQIPMPRVPPPEELPDSSRPPPELLERLPERMRRFFEQPRPFEFR
                     SVQPIDFLRSRISEPTRQLWFRAVGRLPDDEKLHRCLLAYVSDYFLLDTATLPHGTSF
                     LHGSLTMASIDHAMWFHRPLRVDEWLLYATESPSASGARGFARAGLFSLDGRLVASTA
                     QEGLVRIKKAT"
     gene            4486..5541
                     /locus_tag="VFB37_02755"
     CDS             4486..5541
                     /locus_tag="VFB37_02755"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HZO21396.1"
                     /translation="MFAAANTRRGCLVGRMMIVVALAWAFPTGAAAAGTDVDSLQQLW
                     TGIRDSSEEVVVSSEAGINSWPESSERRVRVVVEPVALAWLGPHVLYLEEFLHDDPDN
                     LRRQLLVKLEPAEPPARGVRAELFTFARPRAWVHLNLHPNRLAMLSSADIATIPACDL
                     LFTREGDQFRGSTTGHRCVDARGPTTRYVEYQLLIGQDLYWYRRRLLRKADGEVQEEV
                     IGFNWFELNATQLYTCRIDWAASGRPQDLRPLLRLEVQDQGGHGRFVTPDGKKLELTL
                     HSDDWPFALERDALILVLQEQGAAEPFATAWASADETEIALNLSWLRIRCGPTVPDPD
                     ELQARAPVSRYPSIAVD"
BASE COUNT          998 a         1765 c         1870 g         1076 t
ORIGIN      
        1 agccaggagg tctacacatc ctgatcttgt gcgcggcttt ttccgtgccg cgatgatgcg
       61 cgggacggcc gggcacttcc gtggtgggga tcgcataaag cgtggccgtt gccgggaaaa
      121 tgctccaggg tgacgcagtt ctgcgtccgt ccccgccggc ggagtgtggg atcacttcac
      181 gtgttcgaaa atcgtatgcg atggcatacg tcgcgaccgg cggcgcgttg ttttgcctta
      241 tactagaagc atgatcgaga aaagggaccc atgagcgaga tcgccgatac tttcgactcg
      301 gccttcacca aagccgtcga cctgggaaat cgtctcgcgg acaaggacaa ggacgcggac
      361 ctctgggata tcgcggatgg gctgctcgca ggcgcactgc aatactggct gtactcccga
      421 cagccttgcg gcgacccacg ctgtcaggat tgcatgccca tcagcacggc cgaggggcgc
      481 atggcggagc tcaaaaggct gatggagcaa ctcgcatcgg agagcgagta tttccatacg
      541 ccgaccgacg cgaacgccgg tcgggcttaa gagcgagcgg tttaggcggg cacggcggcg
      601 accgcagccc gcgtgacctc atcgagcgcc tgctcgaaca ggttgaaccc ctctttcaac
      661 agcgcatccg gaatcgtcag tggcgcgaga aaccggatca cgttgccgta gacaccacag
      721 gacaacagga tcagcccgcg ccggctggcc gcttgcacga gagccttggc gaggtccgca
      781 tccggctgct cagcgctgca attcttaacc agttccacgg ccaccatggc cccgagagag
      841 cgcacgtcgc cgatcgcggg aaagcgcacc tgcagtccct tcaggcgcga ggtcatgaaa
      901 tgaccgatgt gctgcgcccg gttcagtagc tgctcctccc ggatgacttc caacacctcc
      961 aagcccgccg cgcacgccag cggcgcgccc gcgtaggtgc ccccaaggcc accgggcatc
     1021 ggcgcgtcca tgatgtccgc cttgccgatc accgcggcca gtggaactcc gcccgcgata
     1081 cttttcgcca ccgtcatgag gtccggctcg accccggcgt gctcgatggc gaacaggcga
     1141 ccggtgcgcg cgaatccagt ctggatctcg tcaatgatga gcacgatgcc gtgctcatcg
     1201 cagatcgagc gcaacttgcg gagcagctcg ggaggcgcga catagaaccc gccttcaccg
     1261 agtaccggct cgatgatgat ggccgccacc cgagccgggt ccacatcggc cttgaagagc
     1321 tggtcgagcg ccttcagcga ctgctccacg gtgatgccgt gataggtcat ggggaagggc
     1381 aggtggaaca cctccggcat catcgggccg aagccggcct tgtagggctg caccttcccc
     1441 gtcaacgcga tgcaggcgag ggtgcggcca tggaaaccac cagaaaaggc gatgacggcg
     1501 gagcgcctgg tgtgaaagcg cgcgatcttg atggcgttct cgaccgcttc ggcgccggtg
     1561 ctcagaaaga tggttttctt cgatgttttg ccgggcgcca gagcgttgag ttgttcggct
     1621 agcgcgacat agctttcgta tggcgtgacc tgaaagcagg tgtgtatgaa ccgatcgagc
     1681 tgccgggcga tcgctccctt gatgcgcgga tggacatgtc ccgtgttgag gacagcgatg
     1741 ccggtgccga aatcgatgta gcgcttgcct tccacgtccc acagctccgc attggaggcg
     1801 cggtcggcat agatcgcgag attgttggcg actccgcgtg ggatggcgtt cttgcggcgc
     1861 tcgtgaagtt ctgcattcga ggtcaagatg gattctctcc agcgcccgtg gcgcgagctc
     1921 tgcgcggatt ctaagccaag tgaggggtgg cctgcttcgg cgcgaccgcg gtcgtggcgg
     1981 catcgcagga actcgcacgt tcatgcgggg gcgccacgga cccactacaa tggtggcttt
     2041 ccttgcagtg gaagtcactg ttttgagcga aaatccttcg atcaccgccg aggtggtcat
     2101 catcggggct ggcccgtgcg ggctgtttca ggtcttcgag ctcgggctgc tcggtatcgg
     2161 cgcgcacatc gtcgactccc tccagcaccc cgggggacaa tgcgcggagc tgtacccgga
     2221 aaagccgatc tacgatattc cggcgctccc ggtctgcgga gctcaggaac tcgtcgatcg
     2281 gctcatgaag cagatcgagc cgttccacgc aactttccac ctcgggcagg aggtcgtcga
     2341 gttcgcgcgc caggagaacg ggcggttcca tgttcgaacc acggcgggca cgacgttcga
     2401 tgccggcgct gtggtgatcg ccgcaggtgt cggctcgttc cagccgcggc ggatcggtgt
     2461 tgagggagca gagcctttcg agggcgtcaa cctgcactac cgcgtcaaga gctccagcgc
     2521 gttccacggt cagcacctcg tcattttcgg tggcggcgat tcggcgctcg actggacgct
     2581 cgagctgctc ccgaaggccg ccagccttac gctggtgcat cggcgtcccg aatttcgtgc
     2641 cgcccccgca tcggtggcga aaatgcagga gcatgtctcc gcgggcagga tgcgattgtt
     2701 cgaggcactg cctcactcgc tcatcctcga cgggggcgcg ctgcggggcg tgaatatcaa
     2761 ggctcgggac ggcgcgatgc acgctctttc cgccgaccag ctcctcgtgt tcttcgggtt
     2821 gcatccgaaa ctgggtgcga tcgccgaatg gggcctggag ctggagaaga aggccttgaa
     2881 agtggatacg gagaagttcc agaccagcct gccgggggtg ttcgccgtgg gtgatatcaa
     2941 cacctacccg gggaagaaga agctgatcct gtcgggcttc cacgaggccg cgctggcggc
     3001 gttcgccatc cagcatcacc tgtatccgca gaagaaacag ttcctgcagt acacgaccac
     3061 gagcccgatc atgcatcagc gtctgggtgt acccgattga attttggccc tagctagagg
     3121 agagaatgat gaagttgata cccaacacac tcgctgcgct ggtggtgctc gcgagtccgg
     3181 cattgttgct gatgtccgct cccgcggccg ccgccggcga tgcttgcaag gtggagatca
     3241 ccggcaacga ccagatgcag tatgacaaga aggagatcga tgttcccgcg tcctgcaagg
     3301 aagtgaccgt gacgctgcat cattcggggc aattgcctgc cgccgcgatg ggacacaact
     3361 gggtgttggt caacacgccg gacctgaccg ccgttgcaaa cgccggcatg ggcgcggggc
     3421 tcgctagcga ttacgttccc gcgggcgaca agcgcgtgct ggcccacacg aaggtggtcg
     3481 gcggtgggca aaccacgagt gtgacgttct cgaccgcggc gctgaaggcg ggcggagact
     3541 actcgtacct gtgtaccttc cccggacaca acgcgctcat gcgcggcaag ttcaagttcg
     3601 gctgaggccg gcatggaccc ctgcatcgcc gatctgttgg ccttgctcga gctcgagcaa
     3661 ctggaggtca acctgtttcg cggcgagagc cgtgatatcg gcagcccgca ggtgtttggc
     3721 gggcaggtgc tcggtcaggc cctcaccgcc gcttcggcga cggtcgaggg ccgagtcgtg
     3781 cactcgctgc acgcctattt tttgcgtcgg ggcgatttca acgcgccgat cgtctaccag
     3841 gtcgaccgca gcctggatgg gcacagcttt tcgaatcgtc gggtggtcgc cattcaacac
     3901 ggccagcaga ttttcaacat ggcagcctcc ttccaggtac ccgaggacgg cttcgaccac
     3961 cagatcccga tgccgcgggt gccgcctccg gaggagctgc cggactcgag ccgaccgcca
     4021 ccggagctgc tggaacgcct gcccgagcgt atgcggcgct tcttcgagca gccccgcccg
     4081 ttcgaattcc gctcggtgca gcccatcgac ttcctccgga gccgcatctc cgaacccacc
     4141 cggcagctct ggtttcgggc ggtgggccgg ttgcccgatg acgagaagct gcatcgttgc
     4201 ctgttggcct acgtctccga ctatttcctg ctcgacacgg cgaccttgcc gcacgggact
     4261 tcgttcctcc atggctctct gaccatggcg agcatcgatc atgcgatgtg gttccatcgg
     4321 ccgctgcgcg tcgacgagtg gctgttgtat gccacggaaa gcccgagcgc ctcgggagcg
     4381 cgtggcttcg cccgtgcggg cctcttcagt ctggacggcc gtctggtggc cagcaccgcc
     4441 caggaaggcc tcgtgcggat caagaaagcc acctagggag cgcgcatgtt cgctgctgcc
     4501 aacacccggc gaggatgtct ggtggggcgg atgatgatcg tcgttgcgct cgcgtgggct
     4561 ttccccaccg gggccgccgc ggcggggacg gatgtcgatt ccctccagca actctggacg
     4621 gggattcgcg attcgagcga ggaggtcgtc gtcagtagcg aggccggcat caactcctgg
     4681 ccggaaagca gcgagcggcg ggtgcgggtg gtggttgagc cggtcgcgct agcctggctt
     4741 ggccctcatg tcctctacct cgaggaattt cttcacgacg atcctgacaa tctgcggcgt
     4801 cagctgctgg tgaagctcga gccggcggag cctccggccc gaggagtacg cgcggagttg
     4861 ttcaccttcg ccaggccacg cgcgtgggtt catctgaatc tccacccgaa tcggctggcc
     4921 atgctgagca gcgcggatat cgccacgatc cctgcctgtg atttgctgtt tacacgcgag
     4981 ggcgatcagt tccggggctc gacgacgggc catcgctgtg tcgatgcgcg cgggccgacc
     5041 acccggtatg tcgaatacca acttctcatt ggccaggatc tctactggta ccgccgccgg
     5101 ttgctgcgca aggccgatgg agaggtgcag gaagaggtca tcggcttcaa ctggttcgag
     5161 ctcaacgcga cccagctcta cacctgtcgg atcgactggg cggcgagcgg acgtccccag
     5221 gacctgcgtc cgctgttgcg tctggaggtc caggaccagg gtggccacgg ccgattcgtg
     5281 acgcccgatg gcaaaaagct ggagctcact ttgcacagcg acgattggcc attcgctctg
     5341 gagcgtgatg ccctgattct cgtgctccag gagcagggcg cggctgagcc cttcgcgacc
     5401 gcttgggctt cggccgatga gacggagatc gcgttgaatc tcagttggct gcggatccgc
     5461 tgtggcccga cggtgccgga cccggatgag ctgcaggcgc gcgcgccagt atctcgatat
     5521 ccttcaatag cagtggattg aaacgctccg gagcctccat catcaggaag ttaccggtgt
     5581 gctcgagcac gtcggccttg aaccccggca ggtacttacg gatgcgcgcc tcgtccgtgg
     5641 gacccaggtc ggagttgatc gcgagcactg gaacgtggat gtccggcagg acggtggtga
     5701 aatcgagtg
//
LOCUS       DASRHN010000071         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_849398, whole genome shotgun sequence.
ACCESSION   DASRHN010000071 DASRHN010000000
VERSION     DASRHN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849398"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFB37_02760"
     CDS             <1..457
                     /locus_tag="VFB37_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21397.1"
                     /translation="WQVNRYVPELERELGFRYASDTRGSGPFIPVVEGRAVGIPQLPT
                     TLPTLDELIGRPDLAATDPISHLLALTGDLLEGDHVYTLHAELEGNTYVGAFERLLRA
                     WRKRGLQLTDLASCAASLDPARLPRCELIAGRVEGRSGQLAYQGEALKA"
     gene            510..1091
                     /locus_tag="VFB37_02765"
     CDS             510..1091
                     /locus_tag="VFB37_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO21398.1"
                     /translation="MNVTLSGAPVISEQDRQISAAVRREGHRLRRFIRRRVLDAAEAE
                     DILQEVLYELVSAYRLLQPIEQVGAWLLRVARNRIIDRFRRTRGEGSFSGSAREDEEA
                     GALEDLLPDIDAGPEGEAMRQVLLEQIEEALDELPREQREVFIAHELEGASYRELSVR
                     WGVNVNTLLARKRYAIAAMRERLQATYDEWLDE"
     gene            1110..1496
                     /locus_tag="VFB37_02770"
     CDS             1110..1496
                     /locus_tag="VFB37_02770"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HZO21399.1"
                     /translation="MRFHRVWRGLGFLVLAVAVLVAVSFVIMGLWNALIPHLFGGPAV
                     SFWEAAGLLVLSRLLFGGFRGGRHGWRHRGWRERWERMSPEERERFRDGFRRWKEMTP
                     EERAEFRRGFSGCGRGMPPGLAHKEG"
     gene            1498..1689
                     /locus_tag="VFB37_02775"
     CDS             1498..1689
                     /locus_tag="VFB37_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21400.1"
                     /translation="MTPGKKPMGAAPLAQDGWELHALEFLERSREVADERIVDAARCE
                     TSTSSRETAAHPGALSPVR"
     gene            1646..2371
                     /locus_tag="VFB37_02780"
     CDS             1646..2371
                     /locus_tag="VFB37_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO21401.1"
                     /translation="MKPPLILVPCLLCDEVVWAAQRAALASVAEVHGALHGAWDSLGA
                     LAEAIVARAPPRFAIAGHSMGGRIALEVARRVPERLTGVALLDTGCEALPRGEPGERE
                     ALKRHALLELARREGMRAMAGTWIQSMIHPARIADETLVNIVLDMFGRRTAEELEVQI
                     RALLRRPDATAVLHQLTCPALVLCGREDLPSPPARHAEMAGQLARATLAVIPECGHMS
                     PLERPQAVSEAMAGWLEAVGRAA"
BASE COUNT          386 a          757 c          924 g          439 t
ORIGIN      
        1 atggcaggtc aaccgatacg ttccggagct cgagcgcgag ctcggattcc gctacgcctc
       61 ggacacgcgc ggcagcgggc cttttattcc ggtagtcgag ggcagggcgg tagggattcc
      121 acaattaccc acgaccttgc ccacactgga tgagttgatc gggcggcccg atctggccgc
      181 tacggatccg atctcgcacc tgctcgcgct cacgggcgat ctgctcgaag gcgaccacgt
      241 gtacacgctg catgctgagc tggagggaaa cacgtacgtg ggggcattcg agcggctcct
      301 ccgtgcctgg cgcaagcgcg gcttgcagct gacggatctg gccagctgcg ccgcctcgct
      361 ggacccggcc cgattgcccc gctgcgagct catcgcgggc cgtgtcgagg ggcgctcggg
      421 gcaattggcc taccagggtg aggccctgaa ggcctgactt ttgtgtcccg gggacactat
      481 ttgccccacc cgttcgtcta tctctacaga tgaacgtcac gctcagcgga gccccggtga
      541 tctccgagca ggatcgacag atctcggccg ccgtgcgccg cgagggccat cgactgcgcc
      601 gcttcattcg gcggcgggtg ctggacgcgg ccgaagcgga agacatcctc caggaggtgc
      661 tttacgagct ggtgtcagcc taccgattgt tgcagcccat cgagcaggtg ggcgcctggc
      721 tactgcgggt ggcccgcaat cggatcatcg atcgattccg tcgcacccgg ggcgagggct
      781 ctttctccgg atctgcgcgt gaggacgagg aggccggtgc gctcgaggac ttgctgccgg
      841 atatcgatgc ggggcccgag ggtgaggcca tgcgccaggt cctgctcgag cagatcgagg
      901 aggcgctcga tgagctgccg cgcgagcaac gcgaggtgtt catcgcacac gagctggagg
      961 gagcgagcta tcgggagctt tctgtgcgtt ggggtgtgaa tgtcaacacg ctgctggcgc
     1021 gcaagcgtta cgcgatcgcc gcgatgcgcg agcgactgca ggcgacatac gacgagtggt
     1081 tggatgagtg aagagtcgag gaggctgtcg tgagatttca ccgggtttgg aggggactgg
     1141 gattcctcgt gctggcggtc gctgtcctgg tggcagtgag cttcgtcatc atggggctgt
     1201 ggaatgcgct catcccgcac ctgttcggcg ggccggcggt gagtttctgg gaggccgccg
     1261 gactgttggt gctgagccgg ctgctgttcg gcggatttcg cggcgggcgg catggctggc
     1321 gacaccgggg ctggcgcgag cgctgggagc ggatgtcgcc cgaggagcgg gagcgctttc
     1381 gcgatggctt ccggcgctgg aaggagatga ccccggagga gcgggccgaa ttccgccgcg
     1441 gattttcggg ctgcggtcgc ggcatgccgc cgggcttggc ccacaaggag ggttgagatg
     1501 actccaggaa agaagccgat gggggcggcc ccgctcgccc aggacggctg ggagctgcac
     1561 gcgctcgaat tcctcgagcg cagccgtgag gtcgccgacg agcggatcgt tgacgcggcg
     1621 cgctgcgaaa cctcaacatc gtcgcgtgaa accgccgctc atcctggtgc cctgtctcct
     1681 gtgcgatgag gtcgtctggg cggctcagcg tgcagcgttg gcttcggtcg cggaggttca
     1741 cggggcgctg cacggtgcat gggacagtct cggagcgctt gccgaggcga tagtggctcg
     1801 agcgccgccg cggtttgcga ttgccgggca ttccatgggc gggaggattg cgctcgaggt
     1861 cgcccggcgg gttccggagc gcctgacggg tgtggcgtta ctcgatacgg gctgtgaggc
     1921 gctgcctcgc ggcgagccgg gagagcggga agcactgaag cgccacgcac tgctggagct
     1981 ggcgcggcgt gagggaatgc gcgcgatggc cggcacctgg attcagagca tgatccatcc
     2041 tgcgcgcatc gccgatgaga cattggtcaa catcgtcctg gatatgtttg gccgccgcac
     2101 ggccgaagag ctcgaagtcc agattcgtgc cttgttgagg aggccggacg ccacagcggt
     2161 gctgcatcaa ctgacttgcc cggctctcgt gctctgcggg cgcgaagatc tcccctcccc
     2221 gccggcgcgc cacgcggaga tggcaggtca gttagctcgc gcgacgctcg ccgtcattcc
     2281 cgaatgcggc cacatgagtc cactggaaag accgcaggcg gtgagcgagg ccatggcggg
     2341 atggctcgag gcggtggggc gcgcagcctg agccggcgcg ctccacgctg cccgataccg
     2401 cccagtcagg ccggggtgcc ggctttcaga ggcagtgcca cggcacgcgt gacggtccgc
     2461 atcgcgaagc tcgactgcac gctcgccaca ctcggcaagc gggtca
//
LOCUS       DASRHN010000072         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_518260, whole genome shotgun sequence.
ACCESSION   DASRHN010000072 DASRHN010000000
VERSION     DASRHN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518260"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFB37_02785"
     CDS             complement(<1..931)
                     /locus_tag="VFB37_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO21402.1"
                     /translation="MLRDGSDPTWEILACSQCGAPLSPEPEGAKCTKCQTRFGHTPHG
                     GLDLRLRATKKFPFEFELGSPPPQDFPFVPLPRNPRPEVEFPAGKVPWHLTPQLLSHF
                     PRARSATSFALDLGCGSGLHRFVCEQAGYRWVGLDYTADRAPILGDGHALPFANESFE
                     FVLSIAVLEHIQYPFVVGREVLRVLKPGGLYIGTVAFLEPFHGNSYYHHTHLGTYNTL
                     RSAGFGVEYVGPNAAWSGLKAQASMGGLFPRMPEALGQAVVWPIEMLHRTWWKMGRLA
                     SRRPNASEQMRLVTNTGAFEFIARKPREDGAAAK"
     gene            complement(951..2282)
                     /locus_tag="VFB37_02790"
     CDS             complement(951..2282)
                     /locus_tag="VFB37_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21403.1"
                     /translation="MNGLLLVVFVVATFADFLTTLPGVPQLVKYVPEAFSCVVAICVL
                     LEGMRTGFSLVAPKYLAVFGLLVLVIVCGILANGDGAGPVLSGTRFYLRGMPLFFLPA
                     VARFSEKQVQQQLKLLLFICLFQVPLAILQRWLIFSAGRFSGDDVRGTVGDSGVLSIV
                     LISAVLVLTGLFMQKRIPRIRYLLLCLLLLFPTTINETKATVILLPIGLFTTLLVAAP
                     SGKRLKITLYTLIGLVAFGSILVPIYDYMNKDSPYKKERNIGDFFTNEKQMVDYMQAK
                     KGVAAVGTTKEVRRGDAIEIPFEYISRDPVRFAFGLGLGNVSPSTLGQNFVGEYNGLF
                     RSFLLTSFTIFLLEIGVLGTALLFLLYWFLGKDALALARSPHPSLVSSIAVGWVGVVV
                     VMFVCLFYTLIHTYSALTYLFWYFSGAVAAHRMQLVLTANRAVIARRRQVV"
     gene            complement(2327..3460)
                     /locus_tag="VFB37_02795"
     CDS             complement(2327..3460)
                     /locus_tag="VFB37_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO21404.1"
                     /translation="MDLTIISTYRCNSRCQMCYIWQNPTDPREEVSLETLSKLPGGFD
                     NLNVSGGEPTLRRDLAELVDLLYPKARVLEISSNGLHPEKLVPIIRKHPNIKVRFSLE
                     GNEHTSNSIRGEKDGHATKMAGLRALQEAGGTDLGFAFVIQDENVDQLVATYEMARDM
                     GVELSTSTLHNAWQFYKNDNYFYDRTKVARQVEGLITSMLSSQKPKNWFRAYLNLGLI
                     EKILGHPRLIRCVAGTGFAFIDPWSDVWTCNVRSDLHMGNLARQSWEEIMGSAEAKQS
                     IKKVHACEQNCWMVTTARTAMRSSLIPQAPKMGPLMWVLKNKLKVSLGLPIAFDRYID
                     YSHVEPSPQKARKSYLNVKQKAVLVRGRNTVDTRYPLRDFQNN"
BASE COUNT          682 a         1046 c         1132 g          606 t
ORIGIN      
        1 ccttggccgc cgcgccatcc tcccgcggct tgcgtgctat gaactcgaag gcgccggtgt
       61 tggtcaccag gcgcatctgc tcgctggcat tgggccgtcg gctggccaga cgccccatct
      121 tccaccacgt ccggtgcagc atttcgatcg gccagacgac cgcctgaccc agggcctcgg
      181 gcatgcgcgg aaagagtccg cccatgctgg cctgcgcctt caaacctgac caagcggcgt
      241 tcggacccac atattccacc ccgaagcctg ccgagcgcag cgtgttgtag gtacccaggt
      301 gggtgtgatg gtagtagctg ttgccgtgga agggttcgag gaaggccacg gtgccgatgt
      361 acaggccacc gggcttcaag actcgcaaca cttcgcggcc gaccacgaag ggatactgga
      421 tgtgctcgag cacggcgatc gagagcacga actcgaagga ctcgttcgcg aagggtaggg
      481 catgtccgtc gcccagaatg ggggctctgt ctgcggtgta gtcgaggccg acccaccggt
      541 agccggcctg ctcgcatacg aagcgatgca gtccactccc gcatccgagg tcgagcgcga
      601 aactcgttgc cgaccgcgcc cgcggaaaat gtgacaggag ctggggcgtg agatgccagg
      661 gcaccttacc cgccggaaat tcaacctcgg ggcgaggatt gcgcggtaga ggcacgaagg
      721 gaaagtcctg cggtggcggg gagccgagct cgaactcgaa ggggaatttc ttggtcgccc
      781 gcaagcgcag atcgaggccg ccgtgcgggg tgtggccgaa gcgcgtctgg cacttggtgc
      841 atttcgcgcc ttccggctcc ggcgacagtg gcgcgccgca ctggctgcac gccaggatct
      901 cccacgtcgg atcggaaccg tcccggagca tcgaactccc tcgaggttgc tcagactacc
      961 tgccgccggc gcgcgatcac ggcgcggttc gcagtcagga ccaactgcat cctgtgcgcg
     1021 gctacggccc ctgaaaagta ccagaacagg tacgtgagcg ccgaataagt atgtattagc
     1081 gtgtaaaaca ggcagacgaa catcaccacg accacgccga cccagccgac ggcaatcgag
     1141 gagacgaggc tcggatgcgg tgatcgcgcc agcgcaaggg catccttgcc aaggaaccag
     1201 tacagcagga agagcagtgc cgtgccgagc acgccgatct ccagcaggaa gatggtgaac
     1261 gaagtcagca ggaagctgcg gaacaggccg ttatactccc cgacgaaatt ctgtccgagc
     1321 gtggagggcg agacgttgcc gagcccgagc ccgaatgcga agcgtaccgg atcgcgtgag
     1381 atgtactcga aggggatctc gatggcatcg ccgcggcgga cttccttggt cgtgccgacg
     1441 gcggccacgc ccttcttcgc ctgcatgtaa tccaccatct gcttctcgtt ggtgaagaag
     1501 tccccgatgt tgcgttcttt cttgtacgga ctgtccttgt tcatgtagtc gtagatgggc
     1561 acgagtatcg agccgaaggc gacgaggccg atgagcgtgt acagcgtgat cttcaagcgc
     1621 ttgccgctcg gggccgccac gagcagcgtg gtgaacaggc cgatcggcag gaggatgacg
     1681 gtggccttgg tttcgttgat cgtggttgga aacagcagca gcaggcacag cagcaggtag
     1741 cggatgcgcg gaatgcgctt ttgcatgaag aggccggtca gcacgagcac cgcgctgatc
     1801 agaacgatcg agagcacacc cgaatcgccc accgttccac gcacatcgtc tccggagaag
     1861 cgtccggcac tgaagatcag ccagcgctgc aggatagcga ggggaacctg gaacaggcag
     1921 atgaacagaa gcagcttcag ttgctgctgg acctgcttct cggagaagcg cgccacggcg
     1981 ggcaggaaga acagcggcat gccacgcaga tagaaccgtg ttccggacag caccggcccg
     2041 gccccatcgc cattggccaa aattccgcag acgatgacga gtaccagcag cccgaagacg
     2101 gcgaggtatt tgggcgcgac cagcgagaac ccggtgcgca tgccctcgag cagcacgcag
     2161 atcgccacga cgcacgagaa cgcctccggg acatacttga cgagttgcgg aacgccagga
     2221 agggtcgtga ggaaatcggc gaaggtcgcc acgacgaaga cgaccaggag aagcccgttc
     2281 attgcggttc agagccgtgt gcggacccag gcatctgcgt acggcctcag ttgttctgga
     2341 agtccctcag cggataacgg gtgtccaccg tattgcgccc gcgtaccaga accgccttct
     2401 gcttcacgtt cagataggac ttgcgggcct tctgcggtga aggctcgacg tgggagtagt
     2461 cgatgtagcg atcgaaggcg atcggcaagc cgagggagac cttgagcttg ttcttcagca
     2521 cccacatgag cggccccatc ttgggggcct gcggaatgag gctcgagcgc atggcagtgc
     2581 gcgcggtggt caccatccag cagttctgct cgcaggcgtg caccttcttg atcgactgct
     2641 tcgcctcggc gctccccatg atctcctccc aggactggcg cgcgaggttg cccatgtgca
     2701 ggtccgagcg gacattgcac gtccagacat ccgaccacgg atcgatgaag gcaaatccgg
     2761 ttccggccac gcagcggatc aggcgcggat ggcccaggat cttctcgatc aggccgagat
     2821 tgaggtaggc gcggaaccag ttcttgggct tctggctgct cagcatcgag gtgatgagtc
     2881 cctcgacctg gcgcgccacc ttggtgcgat cgtagaagta attgtcattc ttgtagaact
     2941 gccaggcatt gtgcagcgtg gaggtcgaga gctcgacgcc catatcgcgc gccatctcgt
     3001 aagtggcgac gagctggtcc acgttctcat cctgaatgac gaaggcgaag ccgaggtcgg
     3061 tgccgccggc ctcctgcagt gcgcgcaggc cggccatctt ggtcgcatgg ccgtccttct
     3121 cgccgcggat gctgttgctg gtgtgctcgt tgccctcgag gctgaagcgc accttgatgt
     3181 tgggatgctt gcggatgatc ggcaccagct tctccgggtg cagcccgttg gagctgatct
     3241 ccagcacgcg cgccttcgga tagagcaggt ccaccagctc cgccagatcc cggcgcagcg
     3301 tcggctcgcc gcccgagaca ttcaggttgt cgaagccgcc cggaagtttc gagagcgtct
     3361 cgagggagac ctcctcgcgc ggatcggtcg ggttctgcca gatgtagcac atctggcagc
     3421 gcgagttgca ccgatacgtg gagatgatcg tcaggtccat tcaaag
//
LOCUS       DASRHN010000073         9971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2003241, whole genome shotgun sequence.
ACCESSION   DASRHN010000073 DASRHN010000000
VERSION     DASRHN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003241"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFB37_02800"
     CDS             <1..746
                     /locus_tag="VFB37_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZO21405.1"
                     /translation="MAGLPKAVRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFAD
                     YTHNLEATGKLVEFSLSDPAVIIGLFIGGLVPYLFGAMAMEAVGRAAGSVVNEVRRQF
                     REIKGIMEGTAKPDYSRAVDMLTKAAIREMIVPSLLPILVPIVLVYVMNAIMGPGAGI
                     KALGGLLIGTIVTGLFVAISMTTGGGAWDNAKKYIEEGNFGGKGSDAHKAAVTGDTVG
                     DPYKDTAGPAINPLIKIINIVALLLVTLL"
     gene            complement(907..1299)
                     /locus_tag="VFB37_02805"
     CDS             complement(907..1299)
                     /locus_tag="VFB37_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO21406.1"
                     /translation="MIEDLPHAEVPLKVLVVDDQADSADAVAFMLSMMGHDVHTAYGP
                     REALELFRELQPAVVFMDISMPELSGLEAAARIRSMPEGTSTTIIALTGLGHEQDRRR
                     SHEASIDHHVVKPASPAVLHRLLSAIHP"
     gene            complement(1452..1751)
                     /locus_tag="VFB37_02810"
     CDS             complement(1452..1751)
                     /locus_tag="VFB37_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21407.1"
                     /translation="MQLARLWREHLAAGCPAELAGEEIAGSDARALDAEITACASALI
                     CRKTTLDGRMERRLYDISAALRDKQSRADARTATYYARLNQLVSAVLVESRTHDA"
     gene            complement(2035..4353)
                     /locus_tag="VFB37_02815"
     CDS             complement(2035..4353)
                     /locus_tag="VFB37_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO21408.1"
                     /translation="MSIVGLLCLLFALVATLLLVLYWIQRREIKGIEQLSQQVRRIAI
                     GGRLEGRVDAGNDQPEIAALATAINHLLTRGASAEREGARGSPKLFAELGDRIHEAVL
                     IHRDVILYANRQFASFVGVDRVELVGRRLGDLVPPEYAELVNENIRRRFAGEPAAERY
                     EIDMVGLQGQLSRLEITSSLIDYEGTPALLITGVEIIPTQTMQQLRLPPDPEVVAAPS
                     MHLLALDSLAEAIIATGATGLISYVNPAAERLTGSSAAQALGKPLEDIVSLVDETDRR
                     LLSDPVRQALTSGAAVNLSRRTLLLSRANGSERSIELSASPIRNEAQALAGAVILLHD
                     VTEMRGLARQMSYQATHDALTGLVNRREFEGRLEEAIESGHRGDGQHVLCYLDLDRFK
                     VVNDTSGHLAGDSMLREVAKVLRDAVRDSDTVARLGGDEFGMLLIGCPLEKARQIADD
                     VCRAVGDYRFVWKDKIFNIGVSIGLVEISRESGTLEELFAAADSACYVAKKQGSGRVA
                     VYSARDEALARHTGEIQWLQKLQSALKENRFQLYHQPIVPAYGTDGGGPATEVLVRLQ
                     DEAGHEVPPSEFVRAAERYRLMSLVDRWVVQTTLAALGRGAMPIPANRSVAINISGQT
                     LGDVQFLEFVVECLDSTGVSPSQVCFEITESAVIANLDHARRFVGVLHGMGCQFAVDD
                     FGSGVGSFSNLKNLPMDYLKIDGSFMRNLAKDSVNQAMVTAMIKLARTLNFKVIAEQV
                     EDEAALEAARRMGVDYVQGFAVGRPQPLPLAA"
     gene            4770..5420
                     /locus_tag="VFB37_02820"
     CDS             4770..5420
                     /locus_tag="VFB37_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZO21409.1"
                     /translation="MQTASAREQMIEQQVRAWDVLDERVLAIFRRIPRERFVPAEQRY
                     LAFADLEIPLPKGQHMLRPSVTGRLLQALELTGTQRVLEVGSGSGFLTACLASVSAYV
                     ESVEVFPELATIAHANLASLGVGNAQVITADATALESDARYEAIAVTASLPVEDERFQ
                     RRLAIGGRLFIVVGEPPVMDARLIRRTSESSWSSQSLFETVIDPLINARRPPEFSF"
     gene            5466..7043
                     /locus_tag="VFB37_02825"
     CDS             5466..7043
                     /locus_tag="VFB37_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZO21410.1"
                     /translation="MNIIQRIAALLAASLFVTGAAHAKDLVGVYEDALQNDPQIRQAN
                     ANRLASREARPQAWASLLPQLSALASRQQDKQSGTQGFLNFDPVTGTYFALPTPFGLN
                     STQKTWSVNLRESLFSWSNWMTLKEADRQVAEAEATYLAAQQNLILRVAQAYFNVLSA
                     QDGLDANQASLEAIQRQLDQADKRFEVGLIAITDVQEAKAARDTAAAAVIAAKRTLAT
                     AGDQLSEVTGQKYDRLSKPGEDMPLTPPEPASEEKWVDASMDQNPTLLSSRLAADVAR
                     DNVRVAFGGHLPTLDLIASKSRTQTSSDESVLGSNPFTLDSNVNDRQYTLQITVPIFS
                     GGLTQSRVRQSQYEWIAAKEQVVQSSRATERQARDAYLGVISGIARVQALRQALQSSE
                     TALKATEAGYEVGTRTAVDVLNARRTLVQAQTDYAGSRYDYIVSVIQLRLAAGSLSRN
                     DVAEINNWLAVTAPTEPAQPIVPPEQSQGAPPPAPPQSPAPPATPQSAIPTPSTPAPT
                     PGQPAAPQPAPSTAPRR"
     gene            complement(7051..8361)
                     /gene="waaA"
                     /locus_tag="VFB37_02830"
     CDS             complement(7051..8361)
                     /gene="waaA"
                     /locus_tag="VFB37_02830"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HZO21411.1"
                     /translation="MRLLYILAVYLAAPIVCAVMLCRGFRDRSYWHNFRERFGFGARL
                     ETEAIWVHAVSVGEVQAAAPLVSTLHERHPALPLLVTTLTPTGAARARALFKHTAQVR
                     YVPFDLPGSVRRFFDRARPRLAVIFETELWPNLYHECSRRRIPLVLASARISSRSVGR
                     YRKLGTLFREALSRGAVVAAQAEGDADRFRSLGADPDRTHVTGNIKFDFCVPTDTAER
                     GEKLRQRYGAGRPVWLAGSTHAGEEEIVLEAHRIVRARHPRALLILVPRHPPRFHDVA
                     ELLRRSSVRYVRRSQDAPPPGAGASASAPVGSAAAAALADVLLVDTLGELLDFYAAVD
                     VAFVGGSLVPIGGHNLLEPAALGVPILTGPNNSNSEDIARMLVERGAAEVVHDASGLG
                     TRVAQLLSEPQQRLSIGQLGRESVDNNRGALAKLLNLIEPLLRA"
     gene            complement(8388..9155)
                     /locus_tag="VFB37_02835"
     CDS             complement(8388..9155)
                     /locus_tag="VFB37_02835"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HZO21412.1"
                     /translation="MKGRWPLGSEVKHTALPGGAMLYDGSRVGNLSPAWFDPKHWQNR
                     GEVEGAAQGRGYALYINSEGRRFVLRHYRRGGLMATVSRDRYYWQGEDVSRPFMEWQL
                     TYRLHRAGLPVPAPLAARYQHEGLTYTGDIITERLATVGSLAECLCKGALSILTWISI
                     GRCIRRFHDLGVCHADLNAHNILLNEDAKAYLIDFDRCQLRRDGLWRDGNLVRLRRSL
                     EKVTYSLPSDRFGEADWHGLLDGYRQLSAEDSPKEGT"
     gene            9205..>9971
                     /locus_tag="VFB37_02840"
     CDS             9205..>9971
                     /locus_tag="VFB37_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZO21413.1"
                     /translation="MSERQRSAALPLTAPPHSVCILRLSALGDTCHVVPLIRTLQHAW
                     PSTRVTWIIGRPEARLMSLLQDVELIVVDKRAGIAAWRDLRNRLAERQFDVLLHLQVA
                     FRASVLSRQVHSPIRLGFDRARARELQWLFTNARIVPQARQHVLDSFFGFTAALGIRE
                     RILEWNLPLPPQAQAYAEHLIPDSQPTLLISPCSSHALRNWQAQRYAAVADHAARVHG
                     MRVILVGGPTLIEREVAAAIEGLVAQKTALINQVGRDT"
BASE COUNT         1804 a         3329 c         3135 g         1703 t
ORIGIN      
        1 agatggccgg gctgccgaaa gccgttcgcg acattactga tccgctcgat gcggtgggca
       61 acaccacgaa agcggtcacc aagggctatg cgatcggatc cgcgggcctt gccgctctcg
      121 tgctgttcgc cgactacact cacaacctcg aggccacggg caagctggtc gaattctctc
      181 tttcggatcc ggccgtcatc atcggcctgt tcatcggcgg tctcgtaccc tacctcttcg
      241 gtgccatggc gatggaggcc gtcggccgcg ccgcgggctc cgtcgtgaac gaggtgcggc
      301 gtcaattccg cgagatcaag gggatcatgg aaggcaccgc caagcccgac tattcgcgcg
      361 ccgtggatat gctcaccaag gcggccatcc gcgagatgat cgtcccctcg ctgctgccca
      421 tcctggtgcc gatcgtgctc gtgtatgtca tgaacgccat catgggaccc ggggccggca
      481 tcaaggctct cggtggtctg ctcatcggca cgatcgtcac cggcctcttc gtggcgattt
      541 cgatgaccac cggcggcgga gcatgggaca acgccaagaa gtacatcgag gaaggtaact
      601 tcggcggcaa gggctccgat gcccacaaag ccgccgtgac aggcgatacg gtgggcgatc
      661 cttacaagga caccgccggt cccgccatca accccctcat caagatcatc aacatcgtgg
      721 cgctgctgct ggtcaccctg ctgtagcggc gacagtcgcg ggcagaaagc gccgcaggcg
      781 tctttctgca ggcgatgaat acaacgcggg ggattgagcg tcaaaagcca cgccttttga
      841 tgcgaggccg agggtcacgg caaccgcaag tcgcgggcag aaagcgccgc aggcgtcttt
      901 ctgcacctaa ggatgtatgg cgctcagcag ccggtgcagc accgcgggcg aggccggctt
      961 cacgacgtgg tgatcgatgc tggcctcgtg ggaccggcgc cggtcctgct cgtgtccgag
     1021 tccggtgagc gcgatgatgg tcgtcgaagt accttcgggc atcgagcgga tgcgggcggc
     1081 cgcttccagg ccactcagct cgggcatgct gatgtccatg aagacgacgg ccggctgcaa
     1141 ctccctgaag agctccagcg cctcccgcgg accgtaggcg gtgtgaacgt cgtgccccat
     1201 catcgaaagc atgaaggcca cggcatctgc cgagtcggcc tggtcatcga ccaccaatac
     1261 cttgagcgga acctcggcat ggggcaagtc ttcgatcatt tcggtcctcg acgtctcggc
     1321 aacaaggcag agacgaaaac tgcgccgtag caagattcgt gcgggctcag ggagcgcgcc
     1381 aagatgcgcg aactcttgcg cgcgatgcag aatttagccg gactcgagcg cttgcgcgac
     1441 tggatggctg atcaggcgtc gtgagttcgc gactcgacca gcaccgcgct gaccagctga
     1501 ttgagacggg cataataagt cgccgtgcgg gcatccgccc gcgactgctt gtcccgcagc
     1561 gcggcgctga tatcgtacag ccggcgctcc atgcgtccat ccagggtggt cttccggcag
     1621 atcagggcgc tggcgcaggc ggtgatttcc gcatccaggg ctcgcgcgtc gctgccggcg
     1681 atctcctcgc ctgccagctc cgcgggacaa ccggcggcca gatgttcgcg ccacaagcgg
     1741 gcaagttgca tggtgggcgt cccgtcatcg ttatggtatt ggggaccggg cgcctttctt
     1801 accacggtcg cgggggggca tgcactgtca aataatgcaa agagaccttt gcaggccggt
     1861 gcagggctcg cccaccccac atcccgcgat tgggagagac tggctcggac gtccgtttgg
     1921 acttcaactc attgatcagg aaggcgcttg cgtcttgcct agggctattc ggaacctgcg
     1981 cgttacgctc tgcaggccga cgatccgcgc tttgctgacc cctctttcgc ccgactacgc
     2041 ggccagcggc agcggctgag gtctcccgac agcgaacccc tgtacgtagt ccacgcccat
     2101 cctgcgggcc gcctccagcg ccgcctcatc ctcgacctgc tcggcaatca ctttgaaatt
     2161 cagagtccgc gcgagcttga tcatcgcggt gaccatggcc tgattcaccg agtccttggc
     2221 gagattgcgc atgaacgagc cgtcgatctt gaggtagtcc atcggaaggt tcttgagatt
     2281 cgagaacgag ccgacgcccg agccgaaatc atcgacggca aactggcagc ccatgccgtg
     2341 cagcacgcct acgaagcggc gcgcatgatc gagattggcg atcaccgcgc tctcggtgat
     2401 ctcgaagcac acctgggaag gggacacccc cgtgctgtcc aggcactcga ccacgaactc
     2461 caggaactgc acatccccca gagtctgccc tgagatgttg atggccacgc tgcggttggc
     2521 cggaatgggc atcgcgcccc gccccaaggc agcgagggtc gtctgcacga cccaccggtc
     2581 cacgaggctc atcagcctgt agcgctcggc ggcccgtacg aactccgagg gcggcacctc
     2641 gtggcccgcc tcgtcctgca gtctgaccag cacctcggtt gccggccccc cgccatcggt
     2701 cccgtaggcc ggcacgatcg gctggtgata gagctggaag cgattctcct tcagcgcgct
     2761 ttgcagcttc tgcagccatt ggatctcgcc ggtgtgccgc gccagcgcct catcgcgcgc
     2821 ggagtacacc gcaacgcggc ccgagccctg cttcttggcg acatagcagg ccgagtccgc
     2881 tgccgcgaac agctcctcca gcgtgccgct ctcgcgcgag atctccacga gtccgatcga
     2941 gacaccgatg ttgaatatct tgtccttcca gacgaatcga tagtcgccta ctgcccggca
     3001 cacgtcatcg gcgatctggc gcgccttctc gagcgggcag ccgatgagca gcattccgaa
     3061 ttcgtcgcca cccaggcgcg ccacggtatc gctgtcgcgc acggcatcgc gcaacacctt
     3121 ggccacctcg cgcaacatgc tgtcacccgc gagatggccg ctggtgtcgt tgacgacttt
     3181 gaaacgatcg aggtcgagat aacacagcac gtgctgacca tcgccgcgat ggccgctctc
     3241 aattgcttcc tcgagccgcc cttcgaactc gcgccggttg acgagtcctg tcagggcatc
     3301 gtgtgtcgcc tggtaggaca tctggcgcgc caggcctcgc atctcggtca cgtcgtgcag
     3361 caggatgacg gcgccggcca gggcctgggc ctcgttacgg atgggcgaag cggagagctc
     3421 gatggagcgc tcgctgccgt tggccctcga cagcagcagc gtgcggcggc tgagattcac
     3481 cgccgcccca ctggtgagtg cctgtcgcac cgggtcggag agcagccgcc gatcggtctc
     3541 atccaccagg ctcacgatgt cctcgagagg cttgccgaga gcctgcgcgg ccgagctgcc
     3601 ggtcaggcgc tcggccgccg gattcacgta actgatcagc ccggttgcac cggtcgcaat
     3661 gatcgcctcc gcgagcgagt cgagcgccag caggtgcatg gagggagccg cgacgacttc
     3721 cgggtccggc ggcaggcgaa gctgctgcat cgtctgagtt ggaatgatct ccacccccgt
     3781 gatgagcagc gccggggtgc cctcgtagtc gatcagcgag gaggtgatct cgagacggct
     3841 gagctgaccc tgcagcccta ccatgtcgat ctcgtagcgt tcggcagcag gctcgcctgc
     3901 aaagcgccgc cggatgttct cgttgacgag ctctgcgtac tcgggcggca ccagatcacc
     3961 gagccggcgg ccgaccagct ctacccggtc gacgccgacg aagctggcga actgacgatt
     4021 cgcgtacagg atcacatcac gatggatcag caccgcctca tgaatcctgt cgcccagctc
     4081 tgcaaacagc ttgggtgagc ctcgcgcgcc ctcgcgctcg gcactggcgc cgcgtgtcag
     4141 gagatgattg attgccgtgg cgagcgctgc gatctcgggc tgatcgttgc cggcgtccac
     4201 gcggccctcg agccggccgc cgatcgcaat ccgtcgtacc tgctgcgaga gttgttcgat
     4261 gcctttgatc tcacgccgtt ggatccagta aagcacgagg agcagcgtcg caacgagcgc
     4321 aaacaaaagg cacaacagcc ccacgatcga catctgcact tcccactgcc tcgcttcgcg
     4381 cgctcgcgcc aaaagtcagc gcattcgagc gcggtgctct ccaagggcga aagtcacgcc
     4441 tggtgtccgc gggccgaaag gcccgcaggg gcactttcag caatcaaccg taagggcgcg
     4501 ctcccgcgga tcactctcat tcgtcttgtc atgccgggga tgcggacaaa cgaagcataa
     4561 cgtaggccag ccttcgcccc ggccgtattt caccatatct cgtcgcaacg ccgcttggca
     4621 ggggctgcga aaacccttaa cttctcttgc attttcgctg acacgcaata agtccaatca
     4681 tcaccgggcg cgaaggtccg accttgcgcc cgcaaatgcg gccgaatggc tcgaggccac
     4741 cgcgccgtca tcgaaggagt gccttgacga tgcagaccgc cagtgcccgc gagcagatga
     4801 tcgagcagca ggtgcgtgcc tgggatgtgc tcgatgagcg tgtgctggcg atattccgca
     4861 ggattccgcg agagcgattt gtgcctgccg agcaacggta cctcgccttc gcggatctgg
     4921 agattccgct gccgaaagga caacacatgc tgcgtcccag tgtgacggga cgtcttctgc
     4981 aggcactcga gctcacgggc acgcagcgcg tgctcgaagt cggctccggc tcggggtttc
     5041 tgaccgcctg cctggccagc gtcagtgcgt atgtggagtc cgtggaagtc ttccctgagc
     5101 tcgccacgat tgctcatgcc aatcttgcat ccctgggcgt gggcaacgcg caagtgatca
     5161 ccgccgacgc cacagcgctt gaatccgacg cgcgctacga ggcaatcgcg gtaacagcat
     5221 cccttcctgt ggaggatgag cgttttcaac gtcggctcgc gataggcggg cgcctgttca
     5281 tcgtcgtggg agagcccccg gtcatggatg cgcgcctcat caggcgcacc tcagagagca
     5341 gctggagctc gcagagtctc ttcgagacgg tcattgaccc tctaatcaac gctcggcgcc
     5401 ctccggagtt ctccttctga gggcggggcg ctcggtcggt caaaagataa ctaattgatt
     5461 tcacagtgaa tattattcaa cgtatcgcgg ccttgctcgc cgcgtcgctc ttcgtgacag
     5521 gcgccgctca cgccaaagac ctcgtcgggg tctatgagga tgccctgcag aacgacccgc
     5581 agatccgtca ggccaatgcc aatcgcctgg cctcgcgcga ggcgcgaccc caggcctggg
     5641 ccagcctgct gccgcagctc tccgcgctcg catcgcgaca gcaagacaag cagagcggca
     5701 cccagggctt cctgaacttc gacccggtaa ccggcaccta tttcgctctc ccgacaccct
     5761 tcgggctgaa ttccacccaa aagacctgga gcgtcaatct gcgcgagagc ctgttctcgt
     5821 ggtccaactg gatgacgttg aaggaggcgg accggcaggt cgcggaagcc gaggcgacct
     5881 atctggctgc gcagcagaat ctgatcctcc gcgtggcaca ggcttatttc aacgtcctgt
     5941 ccgcccagga cggcctcgat gcgaaccagg cctctctcga ggccatccag cgccagctcg
     6001 atcaggcgga caagcgcttc gaggtgggcc tgatcgccat caccgacgta caggaagcca
     6061 aggccgcacg tgatacggcg gcagcagcag tgatcgccgc caagcggact ctggccactg
     6121 cgggcgatca gttgagtgag gtcaccggtc agaaatacga ccggctttcc aagcccggcg
     6181 aggacatgcc cctcacaccc cctgagccgg ccagcgagga gaagtgggtt gatgcctcca
     6241 tggatcagaa tccgacgctg ctttcgagcc gtcttgcggc agacgttgcc cgcgacaacg
     6301 ttcgcgttgc cttcggcgga cacttgccga cactcgacct gatagcgagc aaatcccgca
     6361 cgcaaaccag tagtgacgag tcggtgttgg gatcgaatcc gttcaccctg gacagcaacg
     6421 tgaatgaccg ccagtacacg ctgcagatca ctgtccccat cttcagtggc ggactcacgc
     6481 agtcccgggt ccgacagagc cagtacgaat ggatcgcggc caaggagcag gtcgtgcaga
     6541 gctcacgcgc caccgagcgg caggcgcgag atgcctacct gggagtgatc agcggtatcg
     6601 cgcgcgtgca ggcactccgc caggcactgc aatcgagcga gaccgcgctc aaggctacgg
     6661 aagcgggcta tgaggtcggc acgcgcaccg cggttgacgt cctgaatgcc agacgcacgc
     6721 tcgtgcaggc gcagacggac tacgcgggaa gccgctatga ctacatcgtc agtgtgatcc
     6781 agctgcgcct ggcggccggc agtctctcgc gcaacgacgt tgccgaaatc aacaactggc
     6841 ttgcagtcac agcgccgacc gaaccggctc agcccatcgt gccgcccgag cagtcacagg
     6901 gtgccccgcc ccctgccccg ccgcagtccc cggctccccc cgcgactccg cagtctgcaa
     6961 ttccgacgcc ctcgacgccc gccccgaccc cagggcagcc ggcggcgccg caaccggctc
     7021 cctccacggc accgcgccgc tgaacgcggc tcacgcccgc aacaacggct cgatgaggtt
     7081 gagcagcttg gcgagcgcgc cgcgattgtt gtccaccgac tcccggccga gctggccgat
     7141 gctgaggcgc tgctgcggct cgctgagcaa ctgcgccacc ctcgtcccga gtcctgaggc
     7201 atcgtgcacc acttccgcgg cgccgcgctc gacgagcatg cgagcgatgt cctcgctgtt
     7261 ggaattgttg ggtcccgtga gaatgggtac accgagagcg gcgggctcga gcaggttgtg
     7321 acctccgatg ggcaccagac ttccgccgac gaaggccacg tcgacggccg catagaagtc
     7381 cagcaactcc cccagcgtat ccaccagcag cacatcggcc agcgcagctg cggcggcgga
     7441 tccgaccggg gctgaagcgg atgctcctgc cccggggggt ggcgcatcct gcgatcgccg
     7501 cacgtagcgg accgaagagc gccgtaacag ctcggcgaca tcatggaacc gcggcggatg
     7561 ccgcggcacc aggatcagca aggcacgcgg atgccgcgca cgcacgatcc ggtgggcttc
     7621 gaggacgatc tcttcctcac cggcatgggt gctgccggcg agccacaccg gccgcccggc
     7681 gccgtagcgc tggcgcagtt tctcacctcg ttcggccgtg tccgtgggca cacagaagtc
     7741 gaatttgatg ttccccgtga catgcgtgcg gtcgggatcc gcaccgagtg agcggaatcg
     7801 gtcggcatcg ccttctgcct gcgccgcaac gaccgcaccg cgcgacagcg cttcacggaa
     7861 cagcgtcccg agcttgcggt agcggccgac cgagcgggag gagatccgcg cgctcgcgag
     7921 gaccagcgga atgcgccgcc ggctgcactc gtgatagagg ttcggccata actcggtctc
     7981 gaagatgact gccaggcgcg gccgggcacg atcgaagaaa cggcgcaccg atcccggcag
     8041 atcgaaaggg acatagcgga cctgcgccgt gtgcttgaac agcgcccggg cacgcgccgc
     8101 tccggtcgga gtcagcgtcg tgacgagtag cggcagtgcc ggatggcgct cgtgcagcgt
     8161 gctcaccagc ggcgccgccg cctgcacttc cccgaccgag acggcgtgca cccagatggc
     8221 ctcggtctcc agccgcgcgc cgaaaccgaa ccgttcgcgg aagttgtgcc agtagctgcg
     8281 gtcgcgaaac ccgcggcaca gcatcacggc acagacgatg ggtgcggcga ggtagaccgc
     8341 aaggatgtag aggagacgca cgagcggcgg gtcacccgag ggaggcctca ggtcccctcc
     8401 ttcggcgagt cttccgcgga gagctgccga tagccatcca gcagaccatg ccagtcggcc
     8461 tcgccgaagc ggtcgctcgg caggctgtag gtcaccttct ccagagaccg gcgcaaccgc
     8521 acgagattgc cgtcgcgcca caacccgtct cggcggagct gacatcgatc gaagtcgatg
     8581 aggtaggcct tcgcatcctc gttgagcagg atgttatgcg cgttgaggtc cgcatggcag
     8641 acccccagat catggaagcg gcggatgcag cgtccgatgg agatccaggt caggatcgag
     8701 agtgcgccct tgcacaggca ttccgccagt gagccgacgg tcgccagccg ctcggtgatg
     8761 atgtcaccgg tataggtcaa accctcgtgt tggtagcggg ccgccagcgg ggctggaacc
     8821 ggcaggcccg cgcgatgcag tctgtaagtg agctgccatt ccatgaacgg acgcgaaaca
     8881 tcctcgccct gccagtagta tcgatcacgc gagaccgtcg ccatgaggcc gccgcgccgg
     8941 taatggcgca gcacgaaacg ccggccttcg ctgttgatgt acagcgcata gcccctgccc
     9001 tgtgcggcgc cctcgacctc tccgcgattc tgccagtgtt tcggatcgaa ccaggcggga
     9061 ctcaaattgc ccacacggga accgtcatat agcatggcgc cgccgggcag ggcggtgtgc
     9121 ttgacctcgg aaccgagcgg ccagcgcccc ttcatctcga atgctcccga catcaccggc
     9181 ggattttcta ggattgcacg actggtgagt gaacgccaac gatctgcggc gctgccgctc
     9241 accgcgccgc cccacagcgt gtgcatcctc cggttgtcag ccctcggaga tacctgccac
     9301 gtcgtcccgc tgatccggac tctgcagcac gcgtggccct cgacacgagt tacatggatc
     9361 atcggccgcc cggaagcgcg cctgatgagc ctgttgcagg atgtcgagct catcgtcgtg
     9421 gacaagcgcg caggaatcgc cgcctggcgt gatctgcgta accggctggc cgagcgccag
     9481 ttcgatgtgc tgctgcatct gcaggtcgca tttcgggcca gcgtgctgtc gcggcaggtg
     9541 cactcaccca tccgattggg tttcgaccgg gcgcgggcac gcgagctgca atggctgttc
     9601 accaatgcca ggatcgttcc gcaggcacgg cagcacgtgc tggacagttt tttcggcttc
     9661 accgccgcgc tgggtatccg cgagcgcatt ctggagtgga acctgccgtt gcccccgcag
     9721 gcgcaggcat acgccgagca tctgatccca gactcgcagc cgacattgct catcagtccc
     9781 tgttcgagcc atgcgctgcg caactggcag gcccaacgct acgcagcggt cgccgaccat
     9841 gccgcgcgtg tgcacggcat gcgcgtcatc ctggtcggtg gccccacgct gatcgagcgc
     9901 gaggtcgctg cggccatcga aggtctggtg gcgcagaaaa cagctctcat caaccaggtg
     9961 ggccgggaca c
//
LOCUS       DASRHN010000074        12631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1180204, whole genome shotgun sequence.
ACCESSION   DASRHN010000074 DASRHN010000000
VERSION     DASRHN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180204"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1324
                     /gene="ilvC"
                     /locus_tag="VFB37_02845"
     CDS             329..1324
                     /gene="ilvC"
                     /locus_tag="VFB37_02845"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZO21414.1"
                     /translation="MRLYSQKDVDRKALRGARITVLGYGSQGRAHALNLSDSGFDVVV
                     GVRRGGASWKKARRDGLAVAEPAEAVAGADLIAMLVPDLAQRELYESLQKRLARGSTL
                     LFAHGFNIHFRQIRPRKDLDVVLIAPKGPGDLVRRQYQQGRGVPCLLAVAQDPTGKAH
                     ARALAYAHGIGGTRGGVLETTFGEETETDLFGEQAVLCGGATELVVKGFETLVEAGYQ
                     PEVAYYECLHELKLIVDLLHEGGLRKMHEFISETAKYGDLTRGPRVVNDRVKKEMRRI
                     LKEVKSGKFARQWIAENASGRKTYQKLLDQDTSHPIEKVGAALRARMPWLNERRT"
     gene            1343..2923
                     /locus_tag="VFB37_02850"
     CDS             1343..2923
                     /locus_tag="VFB37_02850"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZO21415.1"
                     /translation="MAPDPDRVLIFDTTLRDGEQSPGCSMTQPEKLRVARALAELGVD
                     VIEAGFPAASRGDWESVHAVAREVPGAVICALARCNREDIELAARALQPAASRRLHVF
                     LATSAIHRQYKLNMAQEEILRTAVDGVRMARELCEDVEFSPEDASRTELEFLAQVVEA
                     AIEAGASTVNIPDTVGYTVPDEVAELFRYLRKNVRGIERIRLSVHCHDDLGMAVANSL
                     TAVLAGARQVECTLNGIGERAGNCSLEEVVMALRTREAFFNVRTNIQTTRLYPTSRLV
                     SSITGMPIPRNKAVVGENAFAHEAGIHQHGMLKHHSTYEILRPEDVGLSRRHLVLGKH
                     SGRHAFRDRVKELGFELDELEFNRLFEEFKALADKKKELFDGDIEALVLRAEGSANGP
                     WTLIELKTVADSRSPAEAVVRLRHAAGQTVERQASGDGPVDAAFKAIEAATGISVMLR
                     KFEVHSVSEGEDAQGEAVICVEYNQRTYRGSSVSTNIVESSTQAFLEVVNRIELSQAS
                     ARARDERIGAATVAQAAV"
     gene            2942..3901
                     /locus_tag="VFB37_02855"
     CDS             2942..3901
                     /locus_tag="VFB37_02855"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZO21416.1"
                     /translation="MPIPATQFIWFNGKLVPWEKATVHVLAHALHYGSSVFEGLRAYQ
                     TPGGVAIFRLRDHTRRLFDSAKVYRIGMPFQPEQINEACREVIRANGLTRGAYLRPVV
                     FRGYGEIGVTPKTEPPTEVAIAAWEWGKYLGHESEAQGVDACVSSWNRVAPNTLPALA
                     KAGGNYLSSQLIGAEARRLGFDEGIGLSPDGLVSEGSGENLFLVRDGVLLTPALAHSV
                     LGGITRDTVMQLARRRGLEVREGAIPREMLYLADEAFFTGTAVEITPIRSVDRLPVGA
                     GRRGPITEALQSAFFGLFSGKTSDEWGWLDAVDLTAPRAAAAG"
     gene            3921..5324
                     /gene="leuC"
                     /locus_tag="VFB37_02860"
     CDS             3921..5324
                     /gene="leuC"
                     /locus_tag="VFB37_02860"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZO21417.1"
                     /translation="MHPARTMFEKVWRSHEVLAETADAPAVLYIDLHLIHEVTSPQAF
                     TLLRQRGLRVRRPDLTLATMDHSTPTRTEQLFGGAPIAIESAARQIRELENNCQQSGV
                     ELFGLRHSRRGIVHIIGPELGITQPGKTVVCGDSHTSTHGALGALAFGIGTTEVAHVL
                     ATQCLLQRKPRTLAIEVSGSLRPGVTAKDLILGIIGRIGVAGGTGHVIEYRGGTIRGF
                     DMDQRLTVCNMSIEAGARAGMIAPDDTTFSYLAGRARAPQGEDWERALERWRELPSDA
                     GAQFDREVHIDATAIEPMITYGTNPGMVMPISAAIPRSDDAAFAKSLAYMGLEAGAPI
                     RDKPVSVVFLGSCTNGRISDLRSAAAVMRGRKVAQGVQMLVIPGSQQVKADAEAEGLD
                     RIFKDAGAQWRESGCSMCLGMNGDLVPSGEYSVSTSNRNFEGRQGAGARTLLASPLTA
                     AASAVRGRVTDPRELLP"
     gene            5356..5931
                     /gene="leuD"
                     /locus_tag="VFB37_02865"
     CDS             5356..5931
                     /gene="leuD"
                     /locus_tag="VFB37_02865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZO21418.1"
                     /translation="MSGIANLRSRTVVLPASNIDTDQIIPARFLTTTTREGLGRNLFA
                     DWRYDSAGQPRPDFALNRPEAQGCRILVAGRNFGCGSSREHAAWALLDHGIQAVISTE
                     FADIFRTNSLKNGLLPIVVSEAVSRRLLDHPGLEVSIDLQATTLTLAHGEAVQFPMEA
                     FARYCLLNGIDELGFLLEQRERIAAYESRSA"
     gene            5928..6995
                     /gene="leuB"
                     /locus_tag="VFB37_02870"
     CDS             5928..6995
                     /gene="leuB"
                     /locus_tag="VFB37_02870"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZO21419.1"
                     /translation="MKAQIVSLGGDGIGPEVACEGLRCLQAAARKFAHEFTVLELPFG
                     GAAIEATGEPLPASTLQACLAADAVLLGAIGGPRWSAPEAKVRPEQGLLRLRRELGVY
                     ANLRPVAVHPALAEISTLKPQVLEGVDLLFVRELTGGIYFGDKRRDADGAQDVCIYTV
                     AEVERITRVAARLARQRRRKVTSIDKSNVLETSRLWREVTTRVMRDEFADVALEHLLV
                     DAAAMHLIRQPRQFDVLVTENMFGDILTDEAAMLAGSLGLLPSASVGDGRRGLYEPIH
                     GSAPDIASRGIANPIGMILSVALMLRHSFELTKEADALEAAVARALQSGARTADVAMP
                     GSATLSTREVGDAVLAELQRS"
     gene            complement(6992..8185)
                     /gene="metB"
                     /locus_tag="VFB37_02875"
     CDS             complement(6992..8185)
                     /gene="metB"
                     /locus_tag="VFB37_02875"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HZO21420.1"
                     /translation="MSDRPGQITQAVRAGIEVDETTGAVVPPLHLTSTFAFRGFGEKR
                     AYDYTRSGNPTRDLLAHALAELELGAGAVITATGMAAITLVGYLIPAGARIVAPHDCY
                     GGTYRLFEAWRRRGERQVEFINFGDEAAVRAALAHPAALLWIETPSNPLLRITDIEKF
                     AALGRAAGALTVVDNTFLSPAWQQPLTLGADLVVHSTTKYLNGHSDVVGGAVVAGDPA
                     LHEQLAWWANCLGITGAPFDSYMTLRGLRTLHARLDHHGRNAIALARWLQEQPNVQQV
                     YYPGLPTHPGHEIAQRQQRGFGAVVTMELEGGHDAVREFVRDLECFSLAESLGGVESL
                     VAHPATMTHAAMDPAARQRAGLADGLVRLSIGIEGLEDLQRDLAAGLARAARTLLPVP
                     KLVTA"
     gene            complement(8182..9252)
                     /locus_tag="VFB37_02880"
     CDS             complement(8182..9252)
                     /locus_tag="VFB37_02880"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF006449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HZO21421.1"
                     /translation="MNASVDSLSLTPRQSSASGFSGLADRPAALDCPVREGLLQVPGE
                     TRLHHGGTLAGVRIAWRLAGPATAPVVCALGGISAGRRVCLGDERDEGWWSGIAGPGC
                     ALDSRRYRILSFDYLGGSGQTSGPEPGQSFPSLSTYDQAEVLLRILNHLGLKSVRAIA
                     GGSYGAMVALAFGERHPERVGHLLTIGAADRPHPMATAWRSVQRQVVRFAISCGRSDE
                     GLQLARALAMSTYRSAEEFSARFGGHPSRQDERFVFPVEGYLLARGSDYARRHRPESF
                     LCLSESIDLHRVDASRIFVPTTAVAVREDQLVPLADVRAMVARLPTARLHEISSLYGH
                     DAFLKETEQLRHIFTVALESLS"
     regulatory      complement(9368..9498)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9548..9901)
                     /locus_tag="VFB37_02885"
     CDS             complement(9548..9901)
                     /locus_tag="VFB37_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21422.1"
                     /translation="MRPQRQFRLTPASTALVAQAHKAANSGDFGLAAATIERAMRIEP
                     DNPLLWIELGRVRLNAADYSQADSMGRKALSLATGDPHAQSSAWRLIADSLRALGRNP
                     EAADADKRAEALAAR"
     gene            complement(10066..12312)
                     /gene="mrcB"
                     /locus_tag="VFB37_02890"
     CDS             complement(10066..12312)
                     /gene="mrcB"
                     /locus_tag="VFB37_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HZO21423.1"
                     /translation="MLLLLLIGVYWIYLDRVVTRQFEGRRWTLPAQIYAAPLELYAGL
                     SLSQGELEHELQRLQYRRVDRLLRQGTYREQGSRIEIALRPARFADELRPASTLNITT
                     NGSGIQSLRDSAGHDLPIIRMEPLLIGSIFPIHGEDRIVVTPQEVPPLLPAALQAVED
                     RNFEHHHGIDPMGIIRAMWVDVRAGQIEQGGSTLTQQLVKSYFLNPRQTFGRKLREAA
                     MAISLELHFSKADIMNAYINEIFLGQDGVRAIHGFGLASEFYFGKPLSELDLSEVALL
                     VAVVRGPSYYDPRRHPDRARLRRDLVLKLMAERGIIRATEAQAAQRKALGVTARGAGA
                     YYPAYLDFVRRTLRRDYRNEDLTEAGLRIYTSLEPRAQDEAERALERELGRLDRTRKP
                     QSGAAQLQGAVVVTSPQSGDVIAIVGGRDVGYDGFNRALDARRQMGSLVKPFIYLTAL
                     ESGRYNAASIIQDVPVTIRLQNGKLWQPENFTHQSNGPVPLVRALAESLNLATVGLGL
                     DLGLPKIAQTLQRFGLPSPPAQVPAMLLGAIDVSPLEAAQMYNGLANGGFQTPLRAVR
                     AVISEDGKAIRAFPLKVTPVATPENIYQLDRMLEQVMVKGTGKAARTILPANLVVAGK
                     SGTSSDLRDDWFAGFSGSHLSIVWVGYDDNRPTGFTGSSAALPVWARVMAGLGTSSWS
                     APMPESLAETWINYQSGGQVDTSCAEDAVPIAVPVGTQLAVQPGCGSSGIESIVKRAG
                     EWLRDIMH"
     gene            complement(12363..>12631)
                     /locus_tag="VFB37_02895"
     CDS             complement(12363..>12631)
                     /locus_tag="VFB37_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZO21424.1"
                     /translation="ATGMNSLLRPALYGAYHEIINLTRSDEPATELVNVVGPICESAD
                     VLGHDRLLAPTREGDVLLIANTGAYGQAMSSHYNLREPAAELFI"
BASE COUNT         2143 a         4217 c         4182 g         2089 t
ORIGIN      
        1 gcgttgacac aggctgcccc ggtatggtcc tatcgaggtc gctcccgatt ctttcgcctg
       61 cgcgcatatc ccatggcagc tttcgtatac ggtcaacgat tcgcagcccc cattcgggcg
      121 ccgcgcctcc tgtccgtcac gctcgtactg cttaccggac ttccgctcat tacgagcgga
      181 cggtgcgggg agctcgcgca agcctgagac gaaaccaagg cacagcgagc aagacaccac
      241 aaaaccccgc accggccctc gagccgctgc ggggtttttt actttctgcc tgtcaatcat
      301 tgcggccgcg tgccgtgtgg agagcgagat gaggctttat tcgcagaagg acgtggaccg
      361 caaggcactt cgcggagcgc gcattaccgt gctcggttac ggcagccagg gacgcgcaca
      421 cgcactgaat ctcagcgaca gcggcttcga tgtcgtggtg ggcgtgcgcc gcggcggggc
      481 cagctggaag aaggcgcgcc gcgacggtct ggccgttgcc gagcccgccg aggcggtcgc
      541 cggcgcagac ctcatcgcca tgctcgtccc cgatctcgca cagcgggagt tgtacgagag
      601 ccttcaaaag cgcctggcac gcggcagcac cctgctcttc gcgcacggct tcaacatcca
      661 tttccgccag atccggcccc gcaaggacct ggatgtcgtg ctcatcgccc ccaaggggcc
      721 cggtgacctc gtccggcgcc agtaccagca gggacgcggc gttccctgcc tgctcgcggt
      781 ggcacaggat cccacgggca aggcccacgc gcgggcgctc gcctatgccc acggcatcgg
      841 tggcacgcgt ggcggcgttc tcgagaccac cttcggtgaa gagaccgaga ccgacctgtt
      901 tggcgagcag gccgtcctgt gcggcggcgc gaccgagctc gtcgtgaagg gtttcgagac
      961 gctggtggag gccggctatc agcccgaagt ggcgtattac gagtgtctgc acgagctgaa
     1021 gctcatcgtg gacctgttgc acgaaggtgg actgcgcaag atgcacgagt tcatcagcga
     1081 gaccgccaag tacggcgatc tgacgcgcgg cccacgagtc gtcaatgatc gtgtgaaaaa
     1141 ggagatgcgc cggatcctca aggaggtgaa gagcggcaag ttcgcccgcc aatggatcgc
     1201 cgagaacgcg agcggccgca agacctatca aaagcttctc gatcaggaca cgtcccaccc
     1261 gatcgagaag gtgggcgcgg cgctgcgcgc acgcatgccc tggctgaacg agcggcgtac
     1321 ctgattgaca cgaggatttc tcatggcacc cgatcctgac cgagtcctga tcttcgacac
     1381 cacgctgcgc gatggcgagc agtcccccgg ctgcagcatg acccagcccg agaagctgcg
     1441 cgtggcacgt gccctcgccg agctcggtgt ggatgttatc gaagcgggct ttccggccgc
     1501 ctcgcgcggt gactgggagt ccgttcacgc ggtggcgcgc gaggtccccg gagccgtcat
     1561 ttgcgcgctc gcgcgttgca accgtgagga catcgagctg gccgcccgcg ctctgcagcc
     1621 ggctgcgagc cggcgcctgc atgtgttcct cgccacgagt gccattcacc gccaatacaa
     1681 gctcaacatg gcccaggagg aaatcctgcg cactgcagtg gacggcgtcc gaatggcgcg
     1741 cgagctttgc gaggacgtcg agttctcgcc cgaggacgcc tcgcgcaccg agctcgaatt
     1801 tctcgcacag gtcgtcgagg ccgccatcga ggccggcgcc agcaccgtca acatccccga
     1861 caccgtcggc tacacggtcc ccgatgaggt cgcggagctg ttccgctatc tgcgcaagaa
     1921 cgtccgcggc atcgagcgga tccgtctgtc ggtgcactgc cacgatgatc tcggcatggc
     1981 ggttgcgaac agtctgacgg cggtccttgc cggtgcgcgc caggtcgagt gcacgctcaa
     2041 tggcatcggc gagcgtgccg gcaactgctc actggaggaa gtcgtcatgg cgctcagaac
     2101 gcgcgaagcg ttcttcaacg tccggaccaa catccagacg acgcgcctct atcccacttc
     2161 gcgtctggta tcgagcatca ccggcatgcc gattccacgc aacaaagcgg tcgtggggga
     2221 gaatgcgttc gctcacgagg ccggcatcca tcagcatggc atgctcaagc accattcgac
     2281 ctacgagatc ctgcggcccg aggacgtcgg gctgtcacgc aggcatctcg tgctcggcaa
     2341 gcacagtggc cgacacgcgt tccgcgatcg ggtgaaggag ctcggattcg agctcgacga
     2401 gctcgagttc aaccgcctct tcgaggagtt caaggcgctc gccgacaaga agaaggagct
     2461 gttcgatggg gacatcgagg ctctggtgct gcgcgccgag ggctccgcga acggcccgtg
     2521 gacgctgatc gagctcaaga ccgtggccga cagccgctcc ccggccgagg ctgtcgtgcg
     2581 cctgcgccat gcagccggac agactgtcga gcgccaggcc tcgggtgatg gtccggtcga
     2641 tgcggcattc aaagccatcg aggctgcgac gggcatcagc gtcatgctgc gcaaattcga
     2701 ggtgcacagc gtctccgagg gcgaggatgc gcagggcgag gcggtcatct gcgtggagta
     2761 caatcagcgc acctaccgcg gctccagcgt cagcacgaac atcgtggaat ccagcaccca
     2821 ggccttcctc gaggtcgtga atcgcatcga gctctcgcaa gcgagtgccc gcgcgcgcga
     2881 tgagcgcatc ggagctgcga ccgtggctca agctgccgtc tgatatcgaa ggagtctggt
     2941 catgcccatt ccggccactc agttcatctg gttcaacggc aagctcgtgc cctgggagaa
     3001 agccacggtg cacgtgcttg cgcacgccct gcactacggc tcctcggtgt tcgaaggcct
     3061 gcgcgcgtat cagacccccg gcggtgtggc catcttccgg ctgcgcgatc atacgcggcg
     3121 cctcttcgat tcggccaagg tctatcgcat cggcatgcca ttccaaccgg agcagatcaa
     3181 cgaggcctgc cgcgaagtca ttcgcgccaa tggcctgacg cgcggcgcgt atctgcgccc
     3241 tgtggtgttt cgcggttatg gcgagatcgg tgtgacaccc aagaccgagc cgccgaccga
     3301 ggtcgcgatc gcggcctggg aatggggcaa gtacctcggc cacgagagcg aggcgcaggg
     3361 agtcgatgcc tgcgtctcct cctggaaccg ggtcgctccc aacacgctgc cggcgctcgc
     3421 caaggccggc ggcaattacc tctcgagcca gctgatcggc gccgaggcgc gccgcctcgg
     3481 cttcgatgaa ggaatcgggc tgtccccgga cggcctggtg agcgagggct cgggtgagaa
     3541 cctgttcctg gtgcgcgacg gggtgctgtt gactccggcg ctcgcgcatt cggttctggg
     3601 cggcatcacg cgcgacaccg tcatgcagct ggcgcgccgc cgcggcctcg aggttcgcga
     3661 gggtgccatt ccgcgcgaga tgctctacct ggcagatgag gccttcttca ccggaaccgc
     3721 ggtcgagatt acccccattc gctccgtaga ccgcctgccg gtcggggccg ggcggcgcgg
     3781 gccgatcacc gaggcgctgc agagcgcctt cttcggcctg ttctccggca agacgagcga
     3841 tgagtggggc tggctcgatg cggtcgacct gactgcgccg cgagccgccg ccgcaggctg
     3901 aagggcggag cgcacgccag atgcacccgg cgcgaacgat gttcgagaag gtctggcgca
     3961 gccacgaagt gctcgccgag accgccgatg caccggcggt cctctacatc gatctgcatt
     4021 tgattcacga ggtcacctcg ccgcaggcct tcaccctcct gcgccagcgc gggctgcgtg
     4081 tgcgccgtcc cgatctgaca ctcgccacca tggatcactc cacccccaca cggaccgagc
     4141 agctgttcgg cggggcgccg attgcgatcg aatcggcggc ccggcagatc cgtgagctgg
     4201 agaacaactg ccagcaatcc ggcgtcgagc tcttcgggct gcgccactcg cgccgcggaa
     4261 tcgtgcacat catcggaccg gagctgggca tcactcagcc cggcaagacc gtggtttgcg
     4321 gagacagcca caccagcacg cacggcgcgc tgggtgcact ggcctttggc atcgggacga
     4381 ccgaggtggc tcatgtcttg gcgactcagt gtctgctcca gcgcaagccc cgcacgctgg
     4441 ccatcgaggt ttccggcagc ctgcgtcccg gtgtcacggc caaggacctg atcctcggca
     4501 tcatcggccg catcggcgtg gccggcggaa cgggccacgt gatcgagtat cgcggtggaa
     4561 cgatccgcgg cttcgatatg gatcagcgcc tgacggtgtg caacatgtcg atcgaggccg
     4621 gcgcccgggc aggcatgatc gctcccgacg acaccacttt ttcctacctc gctggcaggg
     4681 ctcgtgcgcc ccagggcgag gactgggagc gcgcgctcga gcgctggcgc gagctgccga
     4741 gtgatgccgg agcgcaattc gaccgcgaag tgcacatcga tgcgacggcg atcgagccga
     4801 tgatcactta tggcaccaac cccgggatgg tgatgccgat cagtgcagcg atcccccgct
     4861 cggatgatgc ggcgtttgcg aaatctctcg cctatatggg gctggaagcc ggcgcaccga
     4921 tccgcgacaa accggtgagc gtggtgttcc tcggaagctg caccaacgga cgcatctccg
     4981 acctgcgcag cgcagcggcc gtgatgcgcg gccgcaaagt ggcccagggc gtgcagatgc
     5041 tcgtcattcc gggctcgcag caggtcaagg ccgatgcgga ggccgaaggc ctcgatcgca
     5101 tcttcaagga cgccggggcg cagtggcgcg aatccggctg ctcgatgtgc ctcggcatga
     5161 acggagatct cgtgccgagc ggggaatatt ccgtgagcac gagcaatcgc aacttcgaag
     5221 gccgccaggg cgccggtgcc cgcaccctgc tggcaagccc cctgaccgcc gcggccagcg
     5281 cggtgcgcgg tcgcgtcacc gatccacgcg agttgctgcc atgagagctg ccgcagcggg
     5341 ctgcgggggc ggcgcgtgag cgggatcgcg aacttgcgct cgcggacggt cgtgctgccg
     5401 gcaagcaaca tcgataccga ccagatcatc ccggcgcgct tcctgacgac cacgacgcgc
     5461 gaaggcctcg ggcgcaacct cttcgccgac tggcgttatg actccgccgg acagccgcga
     5521 ccggattttg cgctcaaccg ccccgaggct cagggctgtc gcattctggt cgcgggccgc
     5581 aacttcggct gtggctcctc gcgcgagcat gcggcctggg cgctcctcga tcacggcatc
     5641 caggcggtca tcagcacgga gttcgccgac attttccgca ccaactcact gaagaacggc
     5701 ctgttgccga tcgtcgtgag cgaggccgtg agccggcggc tgctcgatca tccaggcctc
     5761 gaggtcagca tcgatctgca ggccaccacg ctcactcttg cccatgggga ggcggtgcag
     5821 ttcccgatgg aggccttcgc gcgctactgt ttgctcaatg gaatcgatga gctcggcttc
     5881 ctgctcgagc agcgcgagcg catcgccgct tacgagagcc gctcggcgtg aaggcgcaga
     5941 tcgtgagtct cgggggcgat ggcatcggcc ccgaggtcgc ctgcgaaggg ctgcgctgcc
     6001 tgcaggcggc cgcgcggaag tttgctcacg agttcaccgt cctcgagctt cctttcggcg
     6061 gcgctgcgat cgaagccacg ggcgagcctt tgcccgcgag cactttgcag gcctgcctgg
     6121 cagccgatgc cgtgctgctc ggggcgatcg gcggtccgcg atggtcggcg ccggaggcga
     6181 aggtgcgacc cgagcagggc ctgctgcgcc tgcgccgcga gctgggtgtc tacgccaacc
     6241 ttcgacccgt tgccgtccat ccggcgctgg ccgagatttc caccctcaag ccgcaggtgc
     6301 tcgagggagt cgatctgctc ttcgtgcgcg agctgaccgg cgggatctac ttcggcgaca
     6361 agcggcgcga tgccgacggt gcgcaggacg tctgcatcta caccgtggcc gaggtcgagc
     6421 ggatcacgcg cgttgccgct cggctcgcgc gccagcggcg gcgtaaagtc acctcgatcg
     6481 acaagtcgaa cgtgctggag acctcgcgcc tgtggcgcga agtcacgacc cgggtgatgc
     6541 gcgatgagtt cgccgacgtg gccctcgagc acctgctggt ggacgctgca gccatgcact
     6601 tgatccgtca accgcggcaa ttcgacgtgt tggtcacgga gaacatgttc ggcgacattc
     6661 tgacggacga ggctgcgatg ctcgcgggct ccctcggcct gctcccctcc gcctcagtgg
     6721 gcgacgggcg gcgcggcctg tatgagccga tacacggctc ggcaccggac atcgcctcgc
     6781 gcggcatcgc caatcctatc gggatgatcc tcagcgtcgc gttgatgctg aggcactcgt
     6841 tcgagctcac caaggaggcg gacgccctgg aggcggcggt cgcgcgcgcc ctgcaaagcg
     6901 gagctcgcac ggcggatgtc gccatgccgg gctcagcgac gctctcgacc cgtgaagtgg
     6961 gcgatgcggt cctcgcggag ttgcagcgca gctaagcggt gaccagcttc ggcaccggaa
     7021 gcagcgtgcg ggccgcgcgg gccagcccgg cagccaggtc gcgctgcaga tcctccagac
     7081 cctcgatgcc gatggacagc cggaccaggc catcagcgag ccccgcccgc tggcgcgcgg
     7141 ccggatccat cgccgcgtgc gtcatcgtcg caggatgggc cacgaggctt tcgactccgc
     7201 cgagcgactc ggcaagcgag aaacattcca ggtctcggac gaactcgcgc accgcatcgt
     7261 gtccgccttc gagctccatc gtgacgacgg caccgaagcc gcgttgctgg cgctgcgcga
     7321 tctcgtgacc gggatgagtg ggtagaccgg ggtaatacac ctgctgcacg ttcggctgct
     7381 cctgcagcca gcgagccagc gctatcgcgt tgcgcccatg gtgatcgagc ctcgcgtgca
     7441 gggtgcgcag accgcgcaag gtcatgtagc tgtcgaacgg cgcgccggtg attcccaggc
     7501 agttggccca ccacgcgagc tgctcatgca gggccgggtc gcctgcgacc acggctccgc
     7561 ctaccacatc actgtggccg ttcagatact tcgtggtgga atgcaccacg agatcggccc
     7621 ccagggtcag gggctgctgc caggctgggg acaggaaggt gttatcgacc acggtgagcg
     7681 cccccgccgc cctgcccagc gctgcaaatt tctcgatgtc cgtgatgcgc agcaatgggt
     7741 tgctcggagt ctcgatccac agcagcgctg ccgggtgcgc cagagcggca cgcacggccg
     7801 cctcatcgcc gaaattgatg aactccacct ggcgctcacc tcggcgccgc cacgcctcga
     7861 acagacggta ggtgccgccg taacaatcgt gcggcgccac gattcgcgcc ccggccggaa
     7921 tcaggtagcc gaccagagtg atggcggcca tgccggtcgc agtgatcacg gccccggcac
     7981 cgagctcgag ctccgccagc gcgtgcgcga gcagatcgcg ggttggatta ccggaacggg
     8041 tgtaatcgta ggcgcgcttc tcgccgaagc cgcggaacgc aaaggtcgag gtgagatgca
     8101 aaggcggcac caccgcaccg gtggtctcat ccacttcgat tccagcgcgt acggcctggg
     8161 tgatctgccc cggacgatcg ctcatgacag actctccagc gccactgtaa agatgtggcg
     8221 taactgttcg gtttccttca ggaaggcatc atgcccatac agcgaagaaa tctcgtgcag
     8281 gcgcgccgtc ggcaagcggg cgaccatcgc ccggacgtcc gcgagtggca cgagctgatc
     8341 ctcccggacc gcgaccgcgg tggtcggaac gaagatgcgc gaggcatcca cgcgatgcag
     8401 atcgatcgat tccgagaggc acagaaacga ctctggccgg tggcggcgcg cgtagtcact
     8461 accgcgcgcc agcagatagc cctcgaccgg aaagacaaag cgctcgtcct gccgcgaggg
     8521 gtggccgccg aagcgggccg agaactcctc cgcgctgcgg taagttgaca tcgcgagcgc
     8581 gcgggccaat tgcaggccct cgtccgagcg accacaggag atcgcgaaac gcacgacctg
     8641 ccgttgcacg ctccgccacg cggtcgccat tggatgaggc cgatcggcgg cgccgatggt
     8701 gagcagatgc ccgacgcgct ccggatgacg ctcaccgaaa gccagtgcca ccatcgctcc
     8761 gtacgagccg cccgcgattg cgcgcacgga cttgagcccg agatgattca ggattcgcag
     8821 cagcacttcg gcctgatcgt aagtggacag actcggaaag ctctggcccg gctcgggccc
     8881 actcgtctgc ccgctgccgc ccaggtagtc aaagctgaga attcggtatc ggcggctgtc
     8941 gagcgcacag ccggggccag cgatccctga ccaccatccc tcgtcccgtt catcccccag
     9001 acacacgcgc cgcccggcgg agatgccgcc gagcgcgcag accacgggcg ccgtggcggg
     9061 cccggccagt cgccacgcga tgcgcacgcc ggccagcgtt ccgccatggt gcaggcgggt
     9121 ctcaccgggt acctgcagca ggccttcgcg cacggggcag tcgagggcgg ctggacgatc
     9181 ggcgagcccc gaaaaaccag acgcgctgct ttggcgcggc gtgagggaaa gagagtcgac
     9241 cgaggcgttc atcaatgctc cgcagacctt gcacggccca ccggcctcct gcggagcgcc
     9301 cgcctctcag gcgggttcgt cgcgtccatc tccgatcgca cgaatggctg atcagagcta
     9361 cgcaaaccgc tcatctgtcg agccggccca gcatgcgccg agtccgaact cgcaggagtt
     9421 ggcaccttcc cagggtggtt aaacctggcg gttgccccgg cgtctaaggg cctatccctc
     9481 agccggtctc gatgagtgat ttgcagtcta tggtgtgccc gacggaggcg tcaagctgac
     9541 gcgagcctta acgggcagcg agtgcctcgg cccgcttgtc cgcatcggcg gcttccgggt
     9601 tacggcccag ggcgcgcagc gagtcggcta tgaggcgcca ggccgagctc tgggcgtgcg
     9661 gatcgccggt cgccagggac agcgccttgc gtcccatgct gtcggcctgg ctgtaatcgg
     9721 cggcgttgag gcgcacgcgt cccagctcga tccacagcaa ggggttgtcc ggctcgatgc
     9781 gcatggcgcg ctcgatggtg gctgccgcca gcccgaaatc accgctattc gccgccttgt
     9841 gcgcctgggc caccagtgcc gtggatgccg gtgtgagccg gaactgacgc tgaggtctca
     9901 tggcagaggc tgggggctgc cccggcgggg gtggttgcgc cgttccggat tggctcggtt
     9961 gctcgggaaa ctgcggcggc ggcgagcgca gcgaatcgca cccggccatc gcgcagcagg
    10021 cggccacgcc cagcgtgagc agcagtttcc cggaggaggc gttcatcaat gcatgatatc
    10081 gcgcagccac tcgcctgcgc gcttcacgat cgattcgatg ccgctgctgc cgcagcccgg
    10141 ctgcaccgca agctgagtgc cgaccggcac ggcgatcggc acggcatctt cggcgcagct
    10201 cgtatcgact tggccgccgc tttgatagtt gatccaggtc tcggccagcg actcaggcat
    10261 cggcgcgctc caggagctcg tcccgaggcc tgccatgacc cgcgcccata ctggcagcgc
    10321 agccgatgaa cccgtgaagc ccgtcggccg gttatcgtca tagccgaccc agacgatgct
    10381 caggtgcgaa ccggagaatc ctgcgaacca gtcatcgcgc aggtccgagg aagtgcctga
    10441 cttgcccgcc accaccagat tcgccggcag aatcgtgcgc gccgccttgc cggtgccttt
    10501 caccatgacc tgctcgagca tccgatcgag ctggtagatg ttttccggcg tggccacggg
    10561 tgtcaccttc agcggaaagg cccggatggc cttgccgtcc tcgctgatca cggcacgtac
    10621 cgctcgcaag ggagtctgga agcctccgtt ggccaggccg ttatacatct gcgcagcttc
    10681 cagaggactc acatcgatgg cgcccaggag catggcggga acttgcgccg gcggactcgg
    10741 caggccgaag cgctgcagcg tctgagcaat cttcggcagt ccgagatcca gccccaggcc
    10801 gacggtcgcg aggttcagcg attccgccag cgcgcgaacc agcggcaccg gaccgttgct
    10861 ctgatgggtg aagttctccg gttgccacag cttgccgttt tgcaggcgga tcgtgaccgg
    10921 cacatcctgg atgatgctgg ctgcgttgta gcgtccactt tcgagcgcgg tgagatagat
    10981 gaatggcttg acgagtgagc ccatctgtct gcgggcatcg agcgcacgat tgaagccgtc
    11041 gtagcccaca tcacggcccc cgacgatcgc gatcacatca ccgctctgcg gcgaggtgac
    11101 gacgacagcc ccttgcagtt gcgccgcccc ggactgtggt ttgcgcgtac gatcgagtcg
    11161 ccccaactca cgctcgagcg cccgctcggc ctcatcctgg gctcgaggct cgagactcgt
    11221 gtagatgcgc agtccggcct ccgtgagatc ctcattccgg taatcgcgac gcagggtgcg
    11281 gcgcacgaaa tcgaggtatg ccggatagta ggcacccgcg ccccgcgcgg tgactccgag
    11341 cgctttcctt tgcgcagcct gggcctcagt ggcgcggatg atgccgcgct ctgccatcag
    11401 cttgagaacc agatcgcggc gcagtctcgc ccggtccgga tgtcggcgcg ggtcgtaata
    11461 cgaaggcccc cggacgacgg cgaccagcag agccacttcc gagagatcca gctccgagag
    11521 cggcttgccg aagtagaact cgcttgccag accaaatccg tgtatcgcgc gcaccccatc
    11581 ctgacccaga aagatctcgt tgatgtaggc gttcatgatg tccgccttgc tgaagtgcag
    11641 ctcgagcgag atggccatcg ccgcctcgcg cagcttgcgg ccgaaggtct ggcgcgggtt
    11701 gagaaagtag ctcttgacca gctgctgtgt cagcgtgctg cccccctgct cgatctggcc
    11761 agcgcgcaca tccacccaca tggcgcggat gatgcccatg gggtcaatac cgtggtggtg
    11821 ctcgaagttg cggtcctcca ccgcctgtag cgctgccggc aacagcggcg gcacctcctg
    11881 gggcgtgacg acgatgcgat cctccccgtg gatcggaaat atgctgccga tcagcagcgg
    11941 ctccatgcgg atgatcggca agtcgtgtcc tgcgctgtcg cgcaggctct ggatgccgct
    12001 tccattggtc gtgatgttga gtgtcgaggc gggccgcagc tcatcggcaa agcgcgccgg
    12061 ccgcagagcg atctcgatcc gcgagccctg ctctcgatag gtaccctgtc ttagcagccg
    12121 atcgacccgg cggtactgca ggcgctgcaa ctcgtgctcg agctcgccct gcgataggga
    12181 gaggccggca tacagctcga gtggagcggc gtagatctgc gccggcagcg tccagcgccg
    12241 cccctcgaat tgccgcgtca cgacgcgatc gagatagatc cagtacacgc cgatgaggag
    12301 cagcaacagc acagcgccgc ccagcagtgt gcgagtgagc cggcttcgca agccgaggcg
    12361 cgtcagatga acagctccgc ggccggctcg cgcaggttgt aatgcgagct catggcctga
    12421 ccgtaggccc ccgtgttggc gatcagcagc acatcgccct cgcgggtggg cgccagaagg
    12481 cgatcatgtc cgagcacatc cgcgctctca cagatgggtc ccacgacgtt cacgagctcc
    12541 gtggccggct cgtccgagcg cgtgagattg atgatttcat ggtatgcccc atagagcgcc
    12601 ggacgcagca gggagttcat cccagtcgcg a
//
LOCUS       DASRHN010000075         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_191530, whole genome shotgun sequence.
ACCESSION   DASRHN010000075 DASRHN010000000
VERSION     DASRHN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191530"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /gene="uvrA"
                     /locus_tag="VFB37_02900"
     CDS             <1..1625
                     /gene="uvrA"
                     /locus_tag="VFB37_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZO21425.1"
                     /translation="TRLNRAARFVYVAERSLPEVAHLTVGRALQFFSSLNLAGWRGEV
                     AAKIVNDVSQRLRFLVDVGLDYLTLDRSAETLSGGEAQRIRLASQVGSGLTGVMYILD
                     EPSIGLHQRDNERLLGTLKRLRDIGNTVIVVEHDEEAILAADHVVDLGPGAGAHGGHV
                     VAQGTAREIQANPASITGQYLSGKRQIALPRLRAQRSPDRLIRIVGAKGNNLRAVTAE
                     IPLGLFTCITGVSGSGKSTLIIDTLFGHAAAQLNGQMLDAAPCDRVEGLEQIDRVIDI
                     DQSPIGRTPRSNPATYTGLFAPLRELFAAVPEARARGYDAGRFSFNVKGGRCEACQGD
                     GLIQVEMHFLPDVYVPCDVCKGRRYNRETLEIRYRGRNIFEVLEMTVEEALRFFQNIP
                     GVANRLQTLTDVGLSYVRLGQNATTLSGGEAQRVKLARELAKRATGRTLYILDEPTTG
                     LHFHDVAQLLDVLHRLRDEGNTIVVIEHNLEVIKTADWVIDLGPEGGNAGGEIIASGT
                     PEQIAGQAGSYTGEFLRPLLGLNGRQASALSR"
     gene            1754..2059
                     /locus_tag="VFB37_02905"
     CDS             1754..2059
                     /locus_tag="VFB37_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZO21426.1"
                     /translation="MDAVAAPGGLALDPGYMSLSALSPGATGTIVGLGAHAESLSALD
                     RRLLELGFCSGERVEIVAEARPGRDPFVVRIGHTTLALRRREAENIWVEISRPGTLS"
     gene            2056..>3801
                     /locus_tag="VFB37_02910"
     CDS             2056..>3801
                     /locus_tag="VFB37_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HZO21427.1"
                     /translation="MNASVAPALGLGSSPSLSLIGVPNCGKTALFNRLTGSRQKVANY
                     PGVTVERKEGRFVGPRTGRIYRLVDLPGAYSLEPTTLDEAIARDVVLGRHAAEAPPDL
                     LVCVVDATNLRLNLRLVLDLKRLGRPMLIALNMSDVAQQRGYQIDRAALERELGLPVV
                     ETVAVRPGGERALLEAIDQLGFGERLDRIEAPPPATSAQIEATQLEVRRILDAIGYRV
                     PTRLVLLSRLDAVVLHPISGPLLLAVVLFLMFQAVFSWAQLPMSLIQSGIASLSGWLG
                     STMQEGPLRGLLVSGIIAGAGSVLVYLPQILILFLFILALEDSGYLPRAAFMLDRLMG
                     KVGLSGRAFIPLLSSFACAIPGIMAARTIPSSRDRLATIMIAPLMTCSARLPVYALLI
                     GAFIPPRAVGVFNLRGLVLFALYAAGVLSAMSVAFLLKRTLMRGSYQPLLLELPEYRL
                     PVLRNLALGLWERVRIFLSRVGTIILSLMVVLWFLASYPAPPHGATGPAIQYSIAGML
                     GRGLEVIFAPLGFNWQISIALVPGLAAREVAVGALGTVYSMSASNDLESALSPIIAST
                     WSIATGLSLLAWYVFA"
BASE COUNT          599 a         1235 c         1216 g          751 t
ORIGIN      
        1 gcacccggct caaccgtgcg gctcgtttcg tttacgtggc cgagcgcagt ctgcccgaag
       61 tcgcgcacct gacggtgggc cgggcgctgc agttcttcag ttccctgaat ctggcgggct
      121 ggcgcggcga ggtggccgcg aagatcgtca acgacgtttc gcagcgacta cgctttctcg
      181 tggacgtggg cctcgattat ctgacgctcg atcgcagcgc ggagacgctg tccgggggcg
      241 aggcgcagcg cattcgcctc gcgagccagg tcggctccgg cctgaccggt gtcatgtaca
      301 tcctcgatga gccttcgatc ggattacatc agcgcgacaa cgaacggctg ctcggcacac
      361 tgaaacgact gcgcgatatc ggcaatacgg tcatcgtcgt cgagcacgat gaggaagcca
      421 tactcgcagc cgatcatgtg gtggacctgg gaccgggagc cggggcccac ggcggacacg
      481 tcgtcgcgca gggcaccgct cgcgagatcc aggccaaccc ggcctcgatc acgggccagt
      541 acctcagcgg caagcgccag atcgctctgc cgcggttacg cgcccaacgc tcgccggatc
      601 gattgatccg catcgtcggg gccaagggca acaacctgcg tgccgtgacg gcagaaatcc
      661 ctcttggact gttcacctgc atcaccggcg tctccggctc tggcaagtca acattgatca
      721 tcgacacgct gttcggtcat gccgcggcac agctgaacgg ccagatgctc gatgccgcac
      781 cgtgcgaccg cgttgaaggg ctcgagcaga tcgaccgggt gatcgacatc gaccagagcc
      841 cgattggccg caccccgcgc tccaatcccg ccacctacac gggcctgttc gcgccgctgc
      901 gggagctgtt cgctgccgtg cccgaggcac gtgctcgcgg ttacgatgcc ggcaggttca
      961 gtttcaacgt caagggaggg cgttgcgagg cctgtcaggg cgatggcctc atccaggtgg
     1021 agatgcattt tcttcccgac gtgtacgttc cgtgtgatgt ctgcaaggga cggcggtaca
     1081 accgagagac gctcgagatc cgctaccgcg gccgcaatat cttcgaggtt ctggagatga
     1141 cggtggagga agccctgcgc ttcttccaga acattccagg tgtggcgaac aggttgcaga
     1201 cgctcacgga tgttgggctc tcgtacgtcc gcctcggtca gaatgcgacg accctgtcgg
     1261 ggggcgaggc gcaaagggtg aaactcgccc gcgagctggc caaacgcgct accggacgca
     1321 cgctgtacat tctcgatgag cccaccacgg gccttcactt ccacgatgtc gcgcagcttc
     1381 tcgatgtcct tcaccggctg cgcgatgagg gcaatacgat cgtggtcatc gagcacaatc
     1441 tggaggtcat caagaccgcg gattgggtga tcgatctcgg tccagagggc ggcaatgccg
     1501 gcggcgagat catcgcgagc ggcacgccgg agcagatcgc cgggcaggcc ggctcgtaca
     1561 ccggtgagtt tttgcgaccg ctcctgggac tgaatggccg gcaggcgagc gctctgagcc
     1621 ggtgagacgg ctccggtcac gctaagtcaa accggcaggg acccctccgc tggcccgtgg
     1681 gctcctgtag ggggtcgcca cggcctaaca agactcgatt gcaaatgcga acggttctca
     1741 tatactgggc cgcatggatg ccgttgctgc gccgggtgga ctcgcactcg atcccggcta
     1801 catgagtctg agcgccttgt cgccgggagc gaccgggacc atcgtgggcc tcggggcaca
     1861 cgccgagagc ctttcagctc tcgatcgacg gctgctcgag ctgggattct gcagtggcga
     1921 gcgcgtcgag atcgttgcag aggcccgtcc gggccgagat ccctttgtcg tccgaatcgg
     1981 ccacacgacg ctggccctgc gacggcgtga agctgaaaac atctgggtgg agatttcccg
     2041 gcccgggacc ctctcgtgaa cgcctcggtt gccccggctc tgggccttgg ctcgagtccc
     2101 agcctatccc tcatcggggt tccgaactgc ggcaagaccg ccctcttcaa tcgcctcacg
     2161 ggcagtcgcc agaaagtggc caactacccg ggcgtcaccg tcgagcgcaa ggaagggcga
     2221 ttcgtgggcc cgcgcaccgg caggatttat cggctggtcg atcttcccgg tgcctacagt
     2281 ctggagccca cgacgctcga tgaggcaatc gctcgcgacg tggtgctggg tcgtcatgcc
     2341 gccgaggcgc cgcctgactt gctggtctgc gtcgtggatg cgacgaatct gcggctcaat
     2401 ttgcgcctgg tactggatct gaagcggctc ggacgcccga tgttgatcgc gctcaacatg
     2461 agcgacgtgg ctcagcaacg cggctatcag atcgaccggg ctgctctgga acgcgagctg
     2521 gggctgccag tcgtcgagac ggtcgcagtt cgaccgggcg gtgagcgcgc acttctcgag
     2581 gccatcgatc agctggggtt tggcgagcgc ctcgatcgaa tcgaggcccc gccgccggcc
     2641 acctcagccc agatcgaggc gacccagctc gaggtgcggc gcattctcga tgccatcggt
     2701 tatcgcgtcc ccacacgcct ggtgctgctt tcgcgcctcg atgcggtagt cctccaccct
     2761 atctccggtc ccctcctcct ggctgtggtg ctgttcctga tgttccaggc ggtattcagc
     2821 tgggcccagt tgccgatgag cctcattcag tcaggcattg cctcgctcag cgggtggctg
     2881 ggcagcacga tgcaagaggg tccgcttcgc ggactgctcg tcagcggcat catcgctggc
     2941 gccggcagcg tgctcgttta tcttccccag attctgatcc tgttcctgtt catccttgcg
     3001 ctggaggatt ccggatacct gccgcgcgca gctttcatgc tcgatcggct gatgggcaag
     3061 gtgggccttt cggggcgtgc cttcattcct ctgctctcca gtttcgcctg cgccattccc
     3121 ggcatcatgg cggcacgcac cattccctcc agccgcgacc gcctcgcgac gatcatgatc
     3181 gcgccgctca tgacgtgttc ggcgcgcctg cccgtgtacg cccttctgat cggcgccttc
     3241 attccgccgc gtgctgtcgg cgtgttcaac ctgcgcggac tcgtactgtt tgcgttgtat
     3301 gccgcgggcg tgttgagtgc gatgagtgtg gccttcctgc tcaagcggac cttgatgcgc
     3361 ggcagctacc aacctctgct tctcgagctt cccgaatacc gtttgcccgt gctccggaat
     3421 ctggcgctcg ggctgtggga gcgcgtccgc atcttcctga gccgcgtcgg aaccatcatc
     3481 ctgtcgctca tggtcgtgct gtggtttctg gcgagctatc cagccccgcc gcacggtgcg
     3541 acaggtccgg ccatccagta cagcatcgca ggcatgctcg ggcgcggact ggaagtcatc
     3601 tttgcgccgc tgggcttcaa ctggcagatc tcgattgcgc tcgttcccgg ccttgcggcg
     3661 cgcgaagtgg ccgtcggcgc cctcgggacg gtgtactcga tgtcggcttc gaatgatctc
     3721 gagagtgccc tctcgccgat cattgcaagc acctggagca ttgccaccgg gctgtcgctg
     3781 ctcgcctggt acgtgttcgc a
//
LOCUS       DASRHN010000076         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1015031, whole genome shotgun sequence.
ACCESSION   DASRHN010000076 DASRHN010000000
VERSION     DASRHN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015031"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFB37_02915"
     CDS             <1..1082
                     /locus_tag="VFB37_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO21428.1"
                     /translation="LVELTGTLRRREAEAQAVLGGVVEGVYAVDKNRTIRYLNPQAAR
                     LLNVTPGQAVGQFCGDVLKPQLEDGRRPCEHRCPILQARVDVSAKAVEHLRVSDSAPR
                     TTVITSSSMVEGLQVQVIRDETELEAVRRARDSVLANISHEFRTPLAAQLASIELLLE
                     GLSTLRPEQQRDLVRSLERGTLRLTRLIDNLLESVRIESGQLGIRRQSVELAEVVEDA
                     QALVDSLLALRRQTLEVSLPTELPLVSGDKPRLTQVFVNLLANANKFAPEDTVVRIGA
                     EAQTGRVLAWVEDEGPGPAPAEGEAIFARFQRGSEEEPEPGGLGLGLWIVKSIIDRHD
                     GTISMIRTDQGRTRFVISLPAERIE"
     gene            1079..1753
                     /locus_tag="VFB37_02920"
     CDS             1079..1753
                     /locus_tag="VFB37_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21429.1"
                     /translation="MKMLVVDDDRDLRELIGFTLTQASYLVVKAADGPAALRTFEAEA
                     PDLVVLDINMPGASGFQVCEAIRKRSRVPVIMLTVRGEEEDLVRALELGADDYLTKPF
                     SPRTLLARIKALLRRAGMENPTPLQAGRIVLDMDEHTVRIAEGEPVRLTKLELRLLQM
                     LVANAGHTVSTDRLLIQVWGHRSSADRQLLKQLVHRLRQKIERDPATPQLLQTAAGAG
                     YKLVVD"
     gene            1849..3312
                     /locus_tag="VFB37_02925"
     CDS             1849..3312
                     /locus_tag="VFB37_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HZO21430.1"
                     /translation="MTPARTIAIVGGGLSGTLVAANLLRAPHWANTVIVLIERARHVA
                     RGKAYVEREHPYLLNVPAGRMSVHPRSPLDFLWYARRRMPHATAEDFLPRPLYGEYLE
                     STLLQAEREASSHVRLQRLRADVCAIERTPGAGQLRVRFADGHALLADEVVLALGNPS
                     PPAVPGTGSLQSSEHYIADPWAMPLSFRAGERLLLIGTSLTMADVATTAATTTQGTAV
                     IHSISRHGLVPPSQAAFSDGRCRSDGTELLRAASFSMLTLLRAVRELADETERNGGDW
                     REAVTFVRNLAPALWKGLSLKERRRMLRHVRPYWDVHRHRLPGETLTRLRQLRLRQKL
                     HIYAGRLLTSRIVNDEAHLTWRPRGASDSRTLVVDRVINCTGPDHNIHRAKDPLMCSL
                     LSQGLTLADPLGLGLRTGAHGALIDSAGRVSANLFYVGPLLRADHWEATAAHELRDHA
                     EGLAKHLTRADLPAPLGRPQTTPSRHVLALRLGQQAP"
     gene            complement(3445..4479)
                     /locus_tag="VFB37_02930"
     CDS             complement(3445..4479)
                     /locus_tag="VFB37_02930"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZO21431.1"
                     /translation="MSGKALDRLCRAWAARLASLIIAFTVVISAPAQAAQVSLLNVSY
                     DPTRELYTEYNAAFARYWKAKTGQDVRINQSHGGSGKQARSVIDGLSADVVTLGLAAD
                     IDAIATQGKLLPLNWASRLPDNSSPYTSTIVFLVRKGNPKGIRDWGDLVRPGLGVITP
                     NPKTSGGARWNYLAAWAWALRQPGGNATTAKEFVRKLYKNVPVLDTGARGATTTFAQR
                     GIGDVLITWENEALLAIRQLGADKLQIVVPTVSILAEPPVAVVDRVVLRRGTREVAQA
                     YLQYLYSKEGQEIVARHFYRPRDPEVLARYARQFPRLNLVSIADFGGWSKVQATHFAE
                     GGIFDQIYAR"
     gene            complement(4487..5161)
                     /gene="lipB"
                     /locus_tag="VFB37_02935"
     CDS             complement(4487..5161)
                     /gene="lipB"
                     /locus_tag="VFB37_02935"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZO21432.1"
                     /translation="MNAALTQPVSARSREGSPPLVRVRRLGRVEYEPTWRAMQAFTDA
                     RNPSTPDEIWFLEHSPVFTLGMNASEAHVLAPGAIPVVRVDRGGQVTYHGPGQLVVYA
                     LIDLRRAALGVRDIVTALERSVIGLASMYGVSAECRRGAPGIYVSGQKLGSVGIRVRR
                     GASYHGLALNVNLDLEPFSRINPCGYFGLQMTRLVDLGGPGSVADTADLLEPHLLRAL
                     GLSAQP"
     gene            complement(5158..6162)
                     /gene="lipA"
                     /locus_tag="VFB37_02940"
     CDS             complement(5158..6162)
                     /gene="lipA"
                     /locus_tag="VFB37_02940"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZO21433.1"
                     /translation="MADLRSGRKYVTPQGFTAIRDGVKAGAAAATRATLTAGLPKPPW
                     LRAKAPSGAGFQSVRALVKEHRLATVCEEAKCPNIGECWNAGTATIMLMGAVCTRACR
                     FCSVDTGNPRGWLDAEEPANAARTVELMKLRYVVLTSVDRDDLPDGGAAHYAACVRAI
                     KDRNPHTAVEALTSDFRGVLRDVETVVDSGIEVFAQNIETVRRLTHPVRDPRAGYDQT
                     LQVLAHAKDHRGEVLTKSSLMLGLGETDEEIAQTLSDLRAVGVDIVTLGQYLRPTLNH
                     LPIERYVTPEQFDQYREQALALGFLECVSGPLVRSSYRAEQALARNNAGLDNRAFALK
                     "
     gene            complement(6194..>6580)
                     /locus_tag="VFB37_02945"
     CDS             complement(6194..>6580)
                     /locus_tag="VFB37_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HZO21434.1"
                     /translation="PFLVVNGDTWTDVDFAALAARAEADDPSSVTARLIFVSNPPQHP
                     RGDFALEGDRVVERDHDRLTYSGVGVYRPEFFAGCTAGKFPLAPLLRRAIAAGLLRGE
                     LHRGEWWDAGTPERLAALDERLGRPI"
BASE COUNT         1132 a         2146 c         2159 g         1143 t
ORIGIN      
        1 acctggtcga gctcaccggc acgctccgtc ggcgtgaggc ggaggcgcag gcggtcttgg
       61 gcggtgtggt cgaaggcgta tacgcggtgg acaagaaccg caccattcgc tacctgaatc
      121 cccaggcggc gcgccttttg aatgtgacac ccgggcaggc cgtgggacag ttctgtggcg
      181 acgtgctgaa gccccaactg gaagacggcc gtcggccctg cgagcatcgc tgccccatcc
      241 tgcaggcccg ggtcgatgtc agcgccaagg cggtcgagca cctgcgggtg tcggactcgg
      301 cgccgcgcac gacggtgatc accagctcca gcatggtcga gggcctgcag gtgcaggtga
      361 tccgtgatga gaccgagctc gaagcggtcc ggcgtgcgcg cgactccgtg ctcgccaaca
      421 tctcgcacga gtttcgcacc ccactcgctg cgcagctcgc ctcgatcgag ctgctgctgg
      481 agggcctttc gaccttgcgg ccggagcagc aacgggacct ggtcagatcc ctggagcgcg
      541 gcacgctgcg cctgactcgc ctgatcgaca acctgctcga gagcgtacgc atcgagtccg
      601 gacagctcgg aatcagacgt cagagcgtcg agctcgccga agtcgtggag gatgctcagg
      661 ccctggtcga ttcgctgctc gcattgcgcc ggcagaccct ggaagtgtcg ttgcccacgg
      721 agctcccgct tgtcagcggc gacaagccac gactcaccca ggtgttcgtc aacctgctcg
      781 ccaacgccaa caaatttgcg ccggaggata ccgtggtacg catcggcgcc gaggcgcaga
      841 cggggcgcgt gctcgcctgg gtcgaggatg agggtccggg tcccgcaccc gccgagggcg
      901 aggcgatctt cgcccgcttc cagcgaggct ccgaggagga gcccgagccc ggcggcctcg
      961 gcctcggcct gtggatcgtg aaatcgatca tcgaccggca tgacggcacg atctcgatga
     1021 tccgcaccga tcaggggcgc acacgattcg tgatctccct gcccgcggag cgtatcgaat
     1081 gaagatgttg gtcgtggacg acgatcgcga tctgcgggag ctgatcggat tcacgctcac
     1141 gcaggcaagc tatctggtcg tcaaggcggc agatggaccc gccgccttgc gcaccttcga
     1201 agcagaagct ccggacctcg tggtgctgga catcaacatg ccgggggcga gcggctttca
     1261 ggtttgtgag gcgattcgca agcgctcacg ggtgccggtc atcatgctca cggtgcgcgg
     1321 cgaggaggaa gatcttgtcc gggcgctcga gctgggcgcc gacgactacc tcaccaagcc
     1381 gttcagcccg cgcaccttgc tggcgagaat caaggcgctg ctgcgtcgcg ccggcatgga
     1441 aaatcccacg cctctccagg cgggacgtat cgtgctcgat atggatgagc acacggtgcg
     1501 catcgccgag ggcgagcccg tgcggctgac gaagctcgag ctgcggcttc tgcagatgct
     1561 ggtggccaat gccgggcaca cagtcagcac ggaccgcttg ctgattcagg tctggggcca
     1621 ccgcagttca gcggatcgac agctgctgaa gcagctcgtg catcgattac gtcagaagat
     1681 cgagcgcgac ccggctacgc ctcagctcct gcagaccgcg gccggggccg ggtacaagct
     1741 cgtcgtggac tagggccgct tgcacctttt tgtcaccgag gtgttactgg tttgatatgg
     1801 ccgatcggct aggctgagcg atatcgaatc cccccggagg tccggctcgt gacacccgca
     1861 cgtaccatcg cgatcgtcgg tggtggcctg agcggcacgc tcgtggccgc gaacctgctc
     1921 agggcaccgc actgggccaa cacagtgatc gtgctcatcg agcgcgcccg gcacgtggcg
     1981 cgcggcaagg cgtacgtcga gcgcgagcac ccgtacctgc ttaacgtgcc tgcgggacgc
     2041 atgtcagttc atccgcgctc cccactggac tttctatggt atgcgcggcg gcggatgcca
     2101 catgccacgg ctgaggactt tttgccgcgc ccgctctacg gcgagtatct ggaatccact
     2161 ctgttgcagg ccgagcgaga ggcatcctcg catgtgcggc ttcagaggct gcgcgcagat
     2221 gtgtgcgcca tcgagagaac tcccggagcc gggcagcttc gcgtgcgttt tgctgacggc
     2281 catgcgctgc tggccgacga ggtcgtgctc gccctcggta atccttcgcc ccccgccgtg
     2341 ccgggcaccg gaagcctgca gtcgtcagag cactacattg ctgatccgtg ggccatgcct
     2401 ctgtcgttca gagcgggcga gcgtctgttg ctcattggca cgagtctcac gatggccgat
     2461 gtcgctacga cggcagcgac cactacccag ggcacggcag tcattcattc gatctcgcgg
     2521 cacggcctcg tgccgccctc ccaggcagca ttcagcgacg ggcgttgccg ctccgatggt
     2581 actgagttgc tgcgcgccgc gtccttctcg atgctgactt tgctgcgtgc tgttcgggag
     2641 ctcgccgacg aaaccgagcg caacggcggg gactggcgcg aagccgtcac attcgtgcgc
     2701 aatctggcac ctgccctctg gaagggttta tcgctgaaag aacggcggcg gatgctccgg
     2761 catgtacgcc cttactggga tgtccatcgt catcggctcc ccggcgagac ccttacccgg
     2821 ctgcgacagt tgcgcctgcg acagaagtta cacatctacg cgggccgcct gctgacctcg
     2881 cgcatcgtga atgacgaagc tcaccttacc tggcgtccgc gcggagccag cgactcccgg
     2941 acactggtgg tggaccgggt gatcaactgc acaggacccg atcacaacat ccaccgcgcc
     3001 aaggacccgc tcatgtgctc gctgctgtct cagggcctga ccctggcgga tccgctgggc
     3061 ctcggcctcc gcaccggtgc gcatggtgca ttgatcgact ccgcgggccg cgtctctgcg
     3121 aatctctttt acgtgggtcc cttgctgcgc gccgatcatt gggaagccac cgccgcacac
     3181 gagcttcggg atcacgccga gggcctggcg aaacatctga cccgcgccga cctgccggcg
     3241 ccgttggggc ggccacagac aacgccctca cgacatgtgc tcgcgctcag gctcgggcag
     3301 caggcgccgt aggcgtcatt cagactgatg cgttcaacgt ggggcgttga gcggcaaaag
     3361 tcaccccttt tgccgcgggg ccaaggatcg cagcgcccgt aagtcgcggg cagaaagcgg
     3421 cgcagccgtc tttctgcaat caaactaacg cgcgtagatc tggtcgaaaa tcccgccctc
     3481 ggcgaagtga gtggcctgca ccttggacca gccgccgaag tcggcaatgc tgacgaggtt
     3541 gagccgaggg aactgccggg cgtagcgggc gagtacttcg ggatcgcgcg gccggtaaaa
     3601 gtgccgggcc acgatttcct gaccttcctt gctgtaaagg tactgcaggt acgcctgggc
     3661 cacttcgcgt gtgccgcggc gcaggacgac gcgatccacc acggccacgg gcggctccgc
     3721 gaggatactc acggtgggca cgacgatctg cagcttgtcc gcccccagct ggcggatcgc
     3781 tagcaacgcc tcgttctccc atgtaatgag gacgtcaccg attccgcgtt gcgcgaaagt
     3841 ggtcgtcgca ccacgcgcgc cggtgtcgag gaccgggacg ttcttgtaga gcttgcgcac
     3901 gaattctttc gcggtggtgg catttccacc tggctgacgc agtgcccagg cccatgctgc
     3961 caggtagttc cagcgcgcgc cccccgaagt cttcgggttg ggtgtgatga cgccaaggcc
     4021 cggcctgacg aggtcgcccc agtcgcggat ccccttcgga ttgcctttgc gcaccaggaa
     4081 aacgatggtc gaggtgtagg gtgagctgtt gtcggggagg cggctcgccc aattgagcgg
     4141 cagcagtttg ccctgcgtgg cgatggcatc gatgtcggcc gccaggccga gcgtcaccac
     4201 atccgctgag aggccatcga tcaccgaacg ggcctgcttg cccgagcccc catgcgattg
     4261 attgatcctc acgtcctgtc cggtctttgc tttccagtag cgcgcgaagg ccgcgttgta
     4321 ttccgtgtac agctcgcgcg tcggatcgta ggacacattc agaagcgaga cttgcgccgc
     4381 ctgcgcaggg gcactgatca ccacggtgaa ggcgatgatg agggaggcga gtcgagcagc
     4441 ccatgcccga cacaaccgat cgagtgcctt accgctcatg ttgaggctac ggctgcgcgc
     4501 tcagaccgag cgcgcgcaga aggtgcggct cgagcagatc cgcggtgtcg gccacactcc
     4561 ccgggccgcc cagatcgacg agccgggtca tctgcagacc gaagtaaccg cacggattga
     4621 tgcgtgagaa aggttccaga tcgaggttca cattgagtgc gaggccgtgg tagctggcac
     4681 cgcgtctgac gcggatcccg acgctgccca gcttctgacc cgacacgtag atgcccggag
     4741 cgccgcggcg gcattccgcg ctgaccccat acatgctcgc gagaccgatg acgctacgct
     4801 ccagcgcggt gacgatgtca cgcacgccga gagccgcccg acgcagatcg atgagtgcat
     4861 agacgaccag ctggccgggg ccgtgatagg tgacctgccc gccacgatcc acgcgcacga
     4921 ccgggatggc accgggcgcc aacacgtgcg cctcgctcgc gttcataccg agagtgaaga
     4981 ccggcgaatg ctccaggaac cagatttcat cgggcgtcga gggattgcgc gcatcggtga
     5041 acgcctgcat ggcgcgccag gtcggctcgt actcgactcg gcccaggcgt cgcactctga
     5101 cgaggggtgg agagccctca cggctgcgcg ccgaaacggg ttgggtcagt gcagcgttca
     5161 cttgagcgca aacgcgcgat tgtcgagacc ggcgttgtta cgcgcgagcg cctgctcggc
     5221 ccgatagctg gatcgcacga gaggtcccga gacgcactcc aggaatccga gcgcgagcgc
     5281 ctgctcgcgg tactggtcga attgctccgg agtgacgtag cgctcgatcg ggagatggtt
     5341 gagcgtcgga cgcaggtact gacccaaggt cacgatgtca acgcccactg cgcgaaggtc
     5401 gctcagcgtc tgtgcaatct cctcatccgt ctcgcccagc ccgagcatca ggctgctctt
     5461 ggtgagcacc tcgccacgat ggtccttcgc atgcgcgagc acctgcaggg tttgatcata
     5521 gccggcgcgc gggtctcgga ccgggtgcgt gaggcgacga acggtctcga tgttctgtgc
     5581 aaagacctcg atgcccgaat cgactaccgt ctcgacatcg cgcagcacgc cgcgaaaatc
     5641 cgatgtcagc gcctcaacgg cggtatgcgg attccggtcc ttgatggctc tgacacaagc
     5701 agcgtagtgc gcggcgccgc catcgggcag gtcatcccga tcgactgagg tcagcacgac
     5761 atagcgcagc ttcatgagct cgaccgtgcg ggccgcgttc gcgggctcct ccgcgtcgag
     5821 ccagcctcgc gggttaccag tgtcgacgga gcagaagcgg caggcgcgtg tgcacaccgc
     5881 ccccatgagc atgatcgtgg ccgtaccggc gttccagcat tcgccgatgt tgggacactt
     5941 ggcctcttcg cagacagtcg ccaggcggtg ttctttaacg agcgcccgga cagactggaa
     6001 tccggctccc gaaggcgcct tcgcgcgcaa ccagggtggc ttgggcagtc ctgcggtcag
     6061 ggtggcgcgc gtggcggcgg ccgcgccagc cttcacgccg tcgcggatcg cggtaaagcc
     6121 ttgcggggta acatatttcc gcccgctgcg cagatctgcc atggcgccat tgtacgcata
     6181 tccccgtccc gcatcagatg ggccggccca agcgctcatc gagtgccgcc aggcgctcgg
     6241 gggttccggc atcccaccat tcaccacgat gcagctcgcc gcgcaacagt ccggcagcta
     6301 tggcccggcg cagcagcggc gcgagcggaa acttgcctgc cgtgcacccg gcaaagaatt
     6361 cgggccgata aacgccgaca cccgagtacg tcagccgatc gtgatctcgt tcgaccaccc
     6421 ggtccccctc gagggcgaag tcgccgcggg gatgctgcgg agggttgctg acgaaaatca
     6481 gtcgtgccgt cacgctggag ggatcgtcag cttctgcgcg tgcagcgagt gcggcgaaat
     6541 cgacatccgt ccaggtatcg ccgttgacca ccagaaacgg
//
LOCUS       DASRHN010000077         5981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_26885, whole genome shotgun sequence.
ACCESSION   DASRHN010000077 DASRHN010000000
VERSION     DASRHN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26885"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFB37_02950"
     CDS             complement(<1..736)
                     /locus_tag="VFB37_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO21435.1"
                     /translation="MRYRIQTLNHISARGLERLPRERYEIAAEIERPQAILVRSADMH
                     GLSLTDSLLAVGRAGAGVNNIPVSLLSKRGIPVFNSPGANANAVKELVLAGLLIAARN
                     ICPAWAFAQALTGDDEAIEAAVEKGKQRFVGFELPGRSLGVVGLGAVGVEVANSALAL
                     GMKVLGYDPQLTVQRAWQLSSSVEQALSLEDLCARADVISVHVPLNAHTKHLLNETRL
                     KLMRKSGIILNFARAAIVDDRAVVAAL"
     gene            complement(745..1824)
                     /gene="serC"
                     /locus_tag="VFB37_02955"
     CDS             complement(745..1824)
                     /gene="serC"
                     /locus_tag="VFB37_02955"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZO21436.1"
                     /translation="MRVFNFAAGPATLPLEVLQQVREELTDWHGLGSSVMEISHRSAA
                     FVGVAREAEASLRRLLRIPGSYRVLFLQGGASAQFAAVPLNLARADSAVDYINTGAWS
                     RKAIHEARRFARVNIAADEAASHYCTVPEPGALRLTAGAAYVHYTPNETIGGVEFPYV
                     PESGAVPLVADMSSTILSRPIEVERFGLIYAGAQKNIGPAGLCVVIVREDLISRAQPP
                     TPAIWDYRAMADEGSMLNTPPTFAWYVAGLVFKWLEDGGGLAAMAERNRAKAQALYQA
                     IDASGFYRNPVAVPARSWMNVPFMLARPGLDAPFLSEAQAAGLTGLEGHRSVGGMRAS
                     LYNAMPLEGVQALIAFMSEFERRHG"
     gene            complement(1846..4449)
                     /gene="gyrA"
                     /locus_tag="VFB37_02960"
     CDS             complement(1846..4449)
                     /gene="gyrA"
                     /locus_tag="VFB37_02960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZO21437.1"
                     /translation="MADIAREILSVSLEDEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMRELGNEWNKAYKKSARVVGDVIGKYHPHGDNAVYDTIVRMAQPFSMRYLL
                     VDGQGNFGSVDGDLPAAMRYTEVRMSRIAGELLADIDKATVDFTPNYDESEHEPAVLP
                     ARIPNLLVNGQTGIAVGMATNIPPHNLTEVVNACLALLEDPSLPLAALMQHVPGPDFP
                     TGGIINGAQEIATAYRTGRGRLSVRARVQVEEVGKGERQAIVVTELPFQVNKARLIER
                     IAQLVREKQLEGIASDGLRDESDKDGMRIVIELRRGEVTEIVLNNLYAQTPMETVFGI
                     NMVALQDGQPRLMSLKEMLDAFIRHRREVVTRRTIYDLAKARERAHILEGLAVALANI
                     DEIIALIKAAPAPAEARTALLARAWPAGIVPQLLERAGAVSTRPAGLAADAGLNGGTY
                     RLSEVQAQAILDMRLNRLTGLEQEKIITEYRELLEGIRDLADVLARPERLTQVVRTEL
                     IEIREAYGDARRTEINRDHLNLTTEDLIEPQDVVVTLSHAGYAKSQPLTEYQTQRRGG
                     RGKAATAVKDEDFVEKLFVAHTHDTVLCFSNRGKVYWLKVYEMPQAGRNARGRPMVNL
                     LPLEEGEKINALLPIKQYDEQHFVFMATSQGTVKKTPLSAFSRPRTSGIIAVDLHDND
                     RLVGVALTDGQREIILVSSGGKAIRFQEDEVRPMGREAAGVRGIKLGEAQELIALIVV
                     GEGNILTASATGYGKLTPLLEFPVHGRGGQGVIALQTSDRNGTTVAALQVLGGQEIML
                     ISSTGTLVRTSVDEISVLGRNTQGVRLIRLAEGERLTGIERIEQLQGEEEPVGGELAA
                     AEPPAGNPEGG"
     gene            4609..5934
                     /locus_tag="VFB37_02965"
     CDS             4609..5934
                     /locus_tag="VFB37_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO21438.1"
                     /translation="MPPEPVDLIIEPRWLLPVAPANIVLAEHAVAIAAGKIVALGSAE
                     RIRRDFEPRERLVREHHALLPGLVNAHTRAATRLLRGLAVQGPLLRWRFEIVLPAEQR
                     WSGPDFARDGVKLAVAEMLRAGITAFADPSLYPEETARVAATTRMRVAVGLPIADEPS
                     AWADSATAYLARAERLWDEYQSDPWVSPYFAPQGADIRDETLVRIRRVADELDARVAM
                     QIHESIVEVRDSLSQHGHRPLRRLRDLGLLRPGFTAVHMNSLEAEDLQAVGASGICAV
                     ACPQSDLRLGSGICPARQLAAQDIAVGLGTGDPVAVGALDLLAEARTAALACGALPEA
                     GRLSAEDVLYMATLGSATAVGLSAQVGSIEPGKSADLLCLDLNVAGCEPVLRAADAIV
                     FGATRSQVSDVWLSGRAAVSAGKLLAFDEQELAALERQWRQRMRRGSDQ"
BASE COUNT         1000 a         1975 c         1980 g         1026 t
ORIGIN      
        1 cgagcgccgc cacgaccgcc cgatcgtcca cgatggcggc acgggcgaaa ttcaggatga
       61 tcccgctctt gcgcatcagc ttcagtcgag tctcattcag cagatgcttc gtgtgggcat
      121 tgagcggcac gtgcacgctg atgacatccg cgcgcgcgca caggtcctcg agcgagagcg
      181 cctgctccac gctcgaagac aattgccacg cgcgctgcac ggtgagctgc gggtcgtagc
      241 ccagaacctt catcccgagg gcgagcgcgg aattcgcgac ctcgaccccc accgcgccca
      301 gtcccaccac ccccaggctg cggccgggga gctcgaatcc gacgaatcgc tgtttgcctt
      361 tctccacggc cgcctcgatg gcctcgtcgt cgcccgtcag ggcctgcgcg aaggcccatg
      421 cagggcagat gttgcgggct gcgatcagca gtcccgccag caccagctcc ttcacggcgt
      481 tggcattggc gccgggagag ttgaagacgg gaatcccacg tttgctgagc agactcacgg
      541 ggatgttgtt gacgccggca cccgcccgcc ccaccgcgag cagcgaatcg gtcaaggaca
      601 gcccgtgcat gtctgcggat cgcaccagga tggcctgcgg gcgctcgatc tcggcggcga
      661 tctcgtaccg ctcgcgcggc aggcgctcga gtccgcgggc gctgatgtgg ttgagcgtct
      721 ggatgcgata gcgcatggat gacttcagcc gtggcgccgt tcaaactcgc tcatgaacgc
      781 gatcagggcc tgcacgccct ccaatggcat cgcgttgtag agactggcac gcatcccccc
      841 gactgaccga tggccctcca acccggtgag tccggccgcc tgggcctccg acaggaaggg
      901 agcatcgagc ccggggcgtg ccagcatgaa ggggacgttc atccacgagc gggcaggcac
      961 agcgaccgga ttgcgataaa accccgatgc atcgatcgcc tgatagagcg cctgggcttt
     1021 ggcccgattg cgctcggcca tcgccgccaa tccgccgccg tcctcgagcc acttgaacac
     1081 caggcccgcg acgtaccagg caaacgtggg cggtgtgttg agcatcgagc cttcatcggc
     1141 catcgcccgg tagtcccaga tggcaggcgt cggtggctgc gcccggctga tgagatcctc
     1201 gcgcacgatc accacgcaca gcccggccgg gccgatgttc ttctgcgccc cggcatagat
     1261 cagtccgaag cgctccacct cgatcgggcg cgagaggatg gtggaagaca tgtcggcgac
     1321 cagaggcacg gctcccgact cggggacata gggaaactcg actccaccga tcgtctcgtt
     1381 gggggtgtag tgcacatagg cggccccggc ggtcagccgc agcgcacctg gctcgggcac
     1441 ggtgcaatag tgagatgccg cttcgtcagc agcgatgttg acgcgtgcaa agcgcctggc
     1501 ctcgtggatg gcctttctgg accaggcgcc ggtgttgatg tagtccacgg cggagtcggc
     1561 acgggcgagg ttgaggggca ctgccgcgaa ctgcgcgctg gcgccaccct gcagaaacaa
     1621 cacccggtaa gagcctggaa tccgcagcag cctgcgcagc gaggcctcgg cctcccgggc
     1681 gacgccgacg aaagcggcac tgcggtgact gatttccatg accgaggagc ccaggccatg
     1741 ccagtcagtc agctcctcgc ggacctgctg gagcacttcg agaggcagag tagccgggcc
     1801 tgcggcaaaa ttgaacacgc gcatagtgcc tgccgcgcgg aagtctcagc cgccttccgg
     1861 attgcctgcc gggggctccg cagctgcaag ctctccaccg acaggctctt cctcaccctg
     1921 cagctgctcg atgcgctcga tacccgtcaa ccgctcgcct tcggccagac ggatcaggcg
     1981 cacgccctgg gtgttgcggc cgagcaccga gatctcatcg acggaagtgc gcaccagcgt
     2041 acccgtggag ctgatcagca tgatctcctg gccgccgagg acctgcagtg ccgcgaccgt
     2101 cgtgccattg cgatcggagg tctgcagcgc gatcacaccc tggccgccgc ggccatgcac
     2161 cggaaactcc agcagcggcg tcagcttgcc gtaaccggtc gcagaggccg tgaggatgtt
     2221 tccctcgccc accacgatga gcgcgatgag ctcctgtgcc tcgccgagct tgattccgcg
     2281 cacgccggcg gcctcgcgcc ccatgggccg cacctcatcc tcctggaagc ggatggcctt
     2341 gccgccgctg gaaacgagga tgatctcgcg ctgcccatcg gtcagcgcca cgccgaccag
     2401 gcggtcgttg tcatggaggt ccacggcgat gatgccgctg gtgcgagggc gggagaacgc
     2461 actcagcggt gtcttcttga ccgtgccctg actcgtcgcc atgaacacga agtgctgctc
     2521 gtcgtactgc ttgatcggca gcagtgcgtt gatcttctcg ccctcctcca gtggcagcag
     2581 attcaccatc ggccggccgc gcgcattgcg ccccgcctgc ggcatctcgt acaccttcag
     2641 ccagtacacc ttgccccggt tggagaagca cagcacggtg tcatgagtgt gggcgacgaa
     2701 cagcttctcg acgaagtcct catccttcac cgcggtcgcg gccttgcccc gaccgccacg
     2761 gcgctgggtc tgatattcgg tcagcggctg cgacttggcg tagccggcat gcgagagcgt
     2821 caccaccaca tcctgtggct cgatcaaatc ctcggtggtg agattgagat gatcgcggtt
     2881 gatctcggtg cggcgcgcat cgccgtaggc ctcccttatc tcgatgagct cggtacggac
     2941 gacctgcgtg agccgctccg ggcgcgcaag gacatcggcc aggtcgcgga tgccctcgag
     3001 cagctcgcga tactcggtaa tgatcttttc ctgctcgaga ccggtcagcc ggttcagccg
     3061 catgtcgagg atggcctgcg cctggacctc ggaaagccga taggtcccgc cgttcagacc
     3121 ggcgtcggca gcgagccccg cgggacgcgt ggagacggca ccggcccgct ccagaagttg
     3181 cggcacgatg cccgcgggcc atgctcgcgc gagcagcgcg gtgcgcgctt cggccggggc
     3241 cggggcggcc ttgatcagcg cgatgatctc gtcgatgttg gccaaggcga ccgcgaggcc
     3301 ttcgagaatg tgggcccgct cgcgcgcttt cgccagatcg tagatggtgc ggcgggtgac
     3361 gacctcgcgt cgatgccgga tgaaggcatc cagcatttcc ttcagcgaca tcagccgggg
     3421 ctgcccatcc tgcagcgcca ccatgttgat gccgaacacc gtctccatcg gtgtctgcgc
     3481 atagaggttg ttcagcacga tctcggtgac ttcgccgcgc ctgagctcga tgacgatgcg
     3541 catgccatcc ttgtcggact catcgcgcag tccgtcggag gcgatgccct cgagctgctt
     3601 ctcgcgcacc agctgcgcga tacgctcgat caggcgggcc ttgttgacct ggaagggcag
     3661 ctcggtgacc acgatcgcct gccgctcacc cttgccgacc tcctcgacct ggacgcgggc
     3721 gcgcaccgag aggcgccccc ggccggtgcg ataggcggtg gcgatctcct gggctccgtt
     3781 gatgatgccg ccggtcggga agtccggtcc cggaacgtgc tgcatcaaag ctgcaagcgg
     3841 cagcgagggg tcctcgagca gcgccagaca ggcgttgacc acctcggtca ggttgtgagg
     3901 cgggatgttg gtcgccatgc cgacggcgat gcccgtctgg ccattgacca gcaggttggg
     3961 gatgcgcgcc ggcaggactg cgggctcgtg ctccgattcg tcgtagttgg gcgtgaaatc
     4021 gaccgttgcc ttgtcgatgt ccgcgagcag ctcgccagcg atgcgcgaca tgcgcacctc
     4081 ggtgtagcgc atcgccgccg gtagatcgcc gtccaccgag ccgaagttgc cctgcccgtc
     4141 caccagcaga tagcgcatcg agaagggctg agccatgcgc acgatcgtgt cgtacacggc
     4201 attgtccccg tgggggtggt acttgccgat gacatcgccg acgacgcgcg ccgacttctt
     4261 gtaggccttg ttccactcat tgcccagctc gcgcatggcg aacaggacac ggcgatgcac
     4321 gggcttcagt ccatcacgaa cgtcgggcag cgcgcgcccg acgatcacgc tcatcgcata
     4381 gtcaagatac gactgccgca tctcatcttc gagactgacg gacagtattt cgcgggcaat
     4441 gtcggccatc tatgcaagag cggccacgag ggctcggtaa aacgccaatc ttaacacagg
     4501 cgctacagtc cgatccgcgc gcagacaggc ggggaatact tggcggagcg gtccgcaacg
     4561 ggcctcgata tactgcgaag ccgccaatcc ttgcgcgctc ttgcgcaaat gccccccgaa
     4621 ccagtcgatc tcatcatcga accccgctgg ctgctgccag tcgccccggc caacatcgtg
     4681 ctggcggaac acgccgtcgc catcgccgcg ggcaagatcg tcgcgttggg atcagcggag
     4741 cgaatcagaa gggatttcga gccccgcgag cggcttgtgc gcgagcacca tgcgcttctg
     4801 cccggactgg tcaacgctca tacgcgtgca gccacgcgct tgctgcgtgg gcttgcggtc
     4861 caggggccgc tgctgcgttg gcgattcgag atcgtcttgc cggccgagca acgctggagt
     4921 ggcccggact ttgcgcgcga tggagtgaag ctcgccgtcg ccgaaatgct gcgggcgggt
     4981 atcacggctt ttgccgatcc ttcgctgtat cccgaggaga cggcccgggt cgccgccacg
     5041 acgcggatgc gcgtggcggt gggcctgccc atcgccgatg agcccagcgc ttgggccgat
     5101 agcgccaccg cgtatctcgc gcgtgccgag cggctgtggg atgaatatca gtccgatccc
     5161 tgggtgtcgc cctatttcgc gccgcagggc gccgatatcc gcgatgagac gctcgtgcgc
     5221 atccgccggg tcgcggatga gctcgatgcg cgggtcgcaa tgcagattca cgagtccatc
     5281 gtagaagttc gcgacagcct gtcgcagcac ggccaccggc ctttgcgcag gctgcgggac
     5341 ctcggactgc tgcggccggg cttcaccgca gttcacatga actcgctgga ggccgaagat
     5401 ctgcaggcgg tgggtgcgag cggcatctgc gctgtggcct gcccccagtc cgacctcaga
     5461 cttggcagcg ggatctgtcc ggcaagacag ctcgcggcgc aggatatcgc ggtaggtttg
     5521 ggtaccgggg acccggtagc cgtgggtgcc ttggatctgc ttgccgaagc gcgcaccgcg
     5581 gcgctggcgt gcggggcgct accggaggct ggaaggctct cggccgagga tgtgctgtat
     5641 atggcaacac tgggaagtgc aacggcagtg ggactgagcg cgcaggtcgg ctcgatcgag
     5701 cccggcaaga gcgccgatct gctgtgcctc gacctgaatg tcgccgggtg cgagccggtg
     5761 ctgcgggccg ccgatgcgat cgtattcggt gccacccgct cgcaggtgag tgacgtatgg
     5821 ctctcgggac gtgccgcggt gagtgccggg aaacttctgg ccttcgatga gcaggagctg
     5881 gccgcgctcg agcgacaatg gaggcaacgc atgcgaaggg gaagcgacca atgagcacac
     5941 ccggagtgaa ttccgatgcc cgcgagctcg gaaaattcga c
//
LOCUS       DASRHN010000078         5118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1015233, whole genome shotgun sequence.
ACCESSION   DASRHN010000078 DASRHN010000000
VERSION     DASRHN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015233"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="dapB"
                     /locus_tag="VFB37_02970"
     CDS             complement(<1..623)
                     /gene="dapB"
                     /locus_tag="VFB37_02970"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZO21439.1"
                     /translation="MPPRASRLRITVIGASGRMGRAVIRSAAEFPELAIVAAVVSPMS
                     EALGRDAGLLAGVPESGLSVTADLQDALAGSDVAVDFSSAGALPAHIQACRQAQRALL
                     VGTTGHRAEAEAVLAAAAEEIPLLVAANTSLGVAVLTELVRAAAAALPPQFDIEIIEA
                     HHRTKQDAPSGTALALGQVAGQARERPNGVEGGRSRSAGARREGEIG"
     gene            complement(623..1759)
                     /gene="dnaJ"
                     /locus_tag="VFB37_02975"
     CDS             complement(623..1759)
                     /gene="dnaJ"
                     /locus_tag="VFB37_02975"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZO21440.1"
                     /translation="MSKRDYYKVLDVPKNATEAEIKKAYRRLAMKYHPDRNPNDREAE
                     ERFKEAKEACEVLTDAQKRAAYDQYGHAGVEAASRGGRGGFGSGDAFSDIFGDVFGDI
                     FGAARRDGRSQVYRGADLRYELAMDLDQAVFGYSAEIEVPKLSECETCHGSGAAKGSS
                     ASACDTCGGSGQIRISQGFFQLQQTCPRCRGAGTLIKNPCDTCLGQGRVRRTRTLSVK
                     VPAGVDTGDRIRLSGEGEAGRNGGPPGDLYVEVNVREHAIFERDGVHLSCEVPVCFAT
                     AALGGTIEVPTLDGDVTLKVPAETQSGRVFRLRDKGVKPVRGGSRGDLFCRVVVETPV
                     HLSTEQRELIRKLDDSLKRDGTRHAPREQGFFEGVKRFFSGSST"
     gene            complement(1843..3792)
                     /gene="dnaK"
                     /locus_tag="VFB37_02980"
     CDS             complement(1843..3792)
                     /gene="dnaK"
                     /locus_tag="VFB37_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZO21441.1"
                     /translation="MAKVIGIDLGTTNSCVAIMEGGKPRVIENSEGDRTTPSIIAFSK
                     DGEVLVGQPAKRQAVTNPQNTLFAIKRLIGRKFDDEVVQRDIKMVPYKIVRAENGDAW
                     VEVQGKRMAPQEISARVLMKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAASLAYGEDKQGKDRKIAVYDLGGGTFDISIIEIAEVDGERQFEV
                     LSTNGDTFLGGEDFDLRIINFLADEFLKESGVDVRKDPLAMQRLKEAAEKAKIELSST
                     QQTDINLPYITADASGPKHLNIKLTRAKLESLVEDLVQKTVGPCRTAMKDAGVSASDI
                     SEVILVGGQTRMPLVQKYVKDFFGKEPRKDVNPDEAVAVGAAIQAGVLAGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIEKNTTIPTKAAQTFSTADDNQTAVTIHVLQGERDRAQD
                     NKSLGKFDLSDIPPAPRGMPQVEVSFDIDANGILNVSAKDKATGKEQKIVIKASSGLT
                     EDEIKRMVRDAEAHAEEDKRFRELAETRNKADGMTHAVEKSLKDLGDKVDPSERARVE
                     SAISDLRTALKGDDRNVIEKKTEALAQASAAIAQRAYGAQQGAAGAGGPSGGAGAQPG
                     AQSGAPGRDDVVDAEFEEVKDKKAS"
     gene            complement(3904..4455)
                     /gene="grpE"
                     /locus_tag="VFB37_02985"
     CDS             complement(3904..4455)
                     /gene="grpE"
                     /locus_tag="VFB37_02985"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZO21442.1"
                     /translation="MSTDEAGRERTAGEPTAVLPETAAQLVELERLQQALTEAEDRAR
                     NHWEQYLRAVADLDNVRKRAQRDIEAANRYGLEKFAAELLPVQDSLELAVQNAGRADA
                     RALAAGQEATLKLLAKALEKIGIVPIDPLGEPFDPARHEAMLMQESATAEPNSVLQVV
                     QPGYELNGRLLRPARVIVAKGRG"
     gene            complement(4530..>5118)
                     /locus_tag="VFB37_02990"
     CDS             complement(4530..>5118)
                     /locus_tag="VFB37_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HZO21443.1"
                     /translation="LVFNDREVQNRIIQLERYYSPDELKRASNYLNEQFRGRTLQQVR
                     HEILRQLAEAHAHMSQIMLDAISVAQHVFEAGEGEERLEYVIKGETNLMGAAELTSVE
                     KLRRLFEAFNEKRDFLHLLDHSLKAEGVKIFIGHESGYQVLDDCSFVAAPYADGDSVV
                     GVVGVIGPTRMAYERIIPIVDMTAKLLGAALNSRR"
BASE COUNT          780 a         1781 c         1578 g          979 t
ORIGIN      
        1 aacccgattt ccccctcacg gcgtgcgccc gcgctgcggc tcctcccacc ctcgaccccg
       61 ttcggtcgct cgcgtgcctg gccagccacc tggccgagcg cgagggcggt ccccgaaggc
      121 gcgtcctgct tggtacggtg atgggcctcg ataatctcga tgtcgaactg cggcgggagc
      181 gccgctgcgg ccgctcgcac gagctccgtc aggacggcca ccccgagact cgtgttggcc
      241 gccaccagga gcgggatttc ctcagccgcg gccgccaaaa ccgcctcggc ctcggctcga
      301 tggccggtcg tgcccaccag cagggcgcgc tgcgcctggc ggcaggcctg aatatgggca
      361 ggcagggccc cggcactgga aaagtccact gcgacgtcac tgcccgccag ggcgtcctgc
      421 aggtcggccg tcacgctcag acccgactcc gggactccgg cgaggagccc ggcatcccga
      481 ccgagcgcct cgctcatggg cgagaccacg gcagccacga tcgccagctc aggaaactcg
      541 gcggccgagc gaatgactgc gcgacccatc cgcccgcttg cgccaatgac ggtgatccgc
      601 agccgggatg ctcgcggggg catcaggtcg agctgccgga gaagaagcgc ttgacgccct
      661 cgaagaagcc ctgctcacgc ggtgcatgcc gcgtcccgtc gcgcttgagg gaatcatcga
      721 gcttgcggat cagctcccgc tgctcggtcg agaggtgcac cggcgtctcg actacgacgc
      781 ggcagaacag atctccccgt gagccaccgc ggacgggctt cacacccttg tcccggagtc
      841 tgaagacgcg gcccgattgg gtctccgccg gcaccttgag agtgacgtcc ccgtccagcg
      901 tgggcacctc gatggtgcct ccgagagccg ccgtcgcaaa gcacaccgga acctcgcagc
      961 tcagatgcac accatcgcgc tcgaagatgg cgtgctcgcg cacgttgact tccacataca
     1021 ggtctccggg cggcccgcca ttacggcctg cctcgccctc accgctcaag cggatccggt
     1081 ctccggtatc gacgccagcg ggcaccttca cggaaagtgt gcgcgtccgg cggacccgac
     1141 cctgcccaag gcaggtgtcg catgggttct tgatcagagt gcccgcaccc cggcaccggg
     1201 gacaggtctg ctgcagctgg aagaagcctt gcgagatgcg gatctgtcca gacccgccgc
     1261 aggtatcgca tgcggatgcc gagctccctt tcgccgcccc gctgccgtgg caggtctcgc
     1321 actccgacag cttggggact tcgatctcgg ccgagtagcc gaacacggcc tgatcgagat
     1381 ccatggcgag ctcgtagcgc agatcggcgc cgcggtacac ctgcgagcgg ccatcccgac
     1441 gcgccgcgcc gaagatgtct ccgaacacat cgccgaatat gtcgctgaag gcatcgccgc
     1501 tgccgaatcc accgcggccg ccacgactgg ccgcctccac ccccgcatgc ccgtactggt
     1561 cgtaggccgc ccgcttctgc gcgtcggtca acacctcgca ggcctccttg gcctccttga
     1621 agcgctcctc cgcctcccga tcgttcgggt tgcggtcggg gtgatacttc atcgcgaggc
     1681 ggcgatacgc cttcttgatc tctgcctcag tcgcgttctt cggtacgtcg agtaccttgt
     1741 agtagtcacg ctttgacatt tgtagagtta atcagaagca gtgcccccgg aaatacgacg
     1801 caccgcacgg gacgtgcggt gcgtgtcaac tttcatctgt actcaggatg ctttcttgtc
     1861 tttcacttcc tcgaactcgg catccaccac atcgtcgcgt cctggcgccc cggactgggc
     1921 tccgggctga gcgcccgcgc cccccgaggg gccgccggcc ccagccgcgc cctgctgggc
     1981 cccgtacgcc cgctgggcaa tcgcggcgga cgcctgggcc agggcttcgg ttttcttctc
     2041 gataacgttc cggtcgtcgc ccttgagcgc cgtgcgcaga tccgaaatcg ccgactcgac
     2101 acgtgctcgt tcgctcgggt cgaccttgtc gcccagatcc ttcagcgact tttccaccgc
     2161 gtgagtcatg ccatccgcct tgttgcgcgt ctcggccagc tcgcggaatc gcttgtcttc
     2221 ctcggcgtgc gcttcggcat cgcgaaccat ccgcttgatc tcatcctcgg tgaggccgct
     2281 cgaggccttg atgacgatct tctgctcctt gccggtggcc ttgtccttcg ctgacacgtt
     2341 gagaatgccg tttgcatcga tatcgaacga tacctcgacc tgcggcatgc cacgcggcgc
     2401 cggcggaata tccgagagat cgaatttgcc gagggacttg ttgtcctgcg cccgatcgcg
     2461 ctcgccctgc agcacgtgga tggtcaccgc ggtctgattg tcgtccgcag tggagaaagt
     2521 ctgcgcagcc ttggtgggga tcgtcgtgtt cttctcgatc agcttggtca tcaccccgcc
     2581 cagggtctcg ataccgagcg acagcggtgt gacatccagc agcagcacgt ccttcacctc
     2641 accggcgagc acaccggcct ggatcgccgc gcccacggcc accgcctcgt ccggattcac
     2701 atccttgcgc ggctctttgc cgaagaagtc cttcacgtac ttctgcacca gaggcatgcg
     2761 ggtctggccg cctaccagga tcacttcgga gatatcactg gccgacaccc ccgcatcctt
     2821 catggcggtg cggcacgggc ccacggtctt ctgcaccagg tcctccacca gggactcgag
     2881 cttcgcccgg gtcagcttga tattgaggtg cttcggcccc gaggcatcgg cggtgatgta
     2941 gggcagattg atgtcggtct gctgggtcga ggacagctcg atcttggctt tctcggcagc
     3001 ttccttcaaa cgctgcatgg ccagcgggtc cttgcgcaca tcgacgccgg attccttcag
     3061 gaactcatcg gccaggaagt tgatgatgcg caggtcgaag tcctcgccac cgaggaaggt
     3121 gtcgccgttg gtcgagagca cctcgaactg ccgctcgcca tcgacctcgg caatctcgat
     3181 gatcgagatg tcgaaggtgc ctccgccgag gtcatacacc gcgatcttgc ggtccttgcc
     3241 ctgcttgtcc tcgccatagg cgagcgaggc cgccgtcggc tcattgatga tgcgcttgac
     3301 ctcgaggccc gcgatgcgcc cggcatcctt ggtcgcctgt cgctgggaat cgttgaagta
     3361 ggcgggcacc gtgatcaccg cctcggtgac cggctcgccc aggtagtcct cggcggtctt
     3421 cttcatcttc atgaggacgc gcgcggagat ctcctgcggg gccatccgct tgccctgcac
     3481 ctctacccac gcatcgccgt tttcggcacg cacgatcttg taggggacca tcttaatgtc
     3541 ccgctgcacg acctcatcat cgaacttgcg gccgatcagc cgcttgatgg caaaaagcgt
     3601 gttctgcggg ttggtgacgg cctggcgctt cgccggctgc cccaccagga cctctccgtc
     3661 cttgctgaag gcgatgatcg acggggtggt acggtcgccc tcgctgttct cgatcacgcg
     3721 aggcttgccg ccctccatga tggccacgca ggaattggtg gttccgaggt cgatgccgat
     3781 tacctttgcc atgggtgtac tccgcaggga atcttctggt ctgttggttg gacttcttgg
     3841 gtacttgccc ctaccgattc aagtagaggg tcggcgaaaa acaccgcgcc gtccgccccc
     3901 tcctcagccc cggcccttgg cgacgatgac ccgggcaggc cgcagcaggc gcccgttgag
     3961 ctcgtagccg ggctgcacga cctgcagcac cgaattgggc tccgcggtcg ccgactcctg
     4021 catcagcatc gcctcgtggc gagctggatc gaagggctcc ccgaggggat cgataggcac
     4081 gattccgatc ttttccaggg ccttggccag aagcttcagc gtcgcctcct ggccggcggc
     4141 cagggcgcgc gcatccgccc taccggcgtt ctgcacggcc agctccagac tgtcctggac
     4201 cggcagcagc tccgcggcga acttctccag cccgtagcgg ttcgcggcct cgatatcgcg
     4261 ttgggcccgc ttgcggacgt tgtccaggtc tgccaccgca cgcaggtact gctcccagtg
     4321 attgcgggcc cggtcctcgg cctcggtcag cgcctgttgc aggcgctcga gctccacgag
     4381 ctgggccgcc gtctcgggca gcaccgccgt cggctccccc gccgtccgct cccgtccggc
     4441 ctcatcagtg ctcatcggaa tcctcctgcc gtcggatggt tgtcggcgcc gccggtcccg
     4501 acgcgctgaa gaggggcgta actgcggatt cagcgccggg aattcaaggc ggcgccgagc
     4561 agtttcgcag tcatatccac tatggggatg atgcgttcgt aggccatgcg cgtgggtccg
     4621 atgaccccca ccacccccac gaccgagtcg ccgtcggcgt acggggcggc gacgaaactg
     4681 cagtcatcga ggacctggta gcccgattcg tgtccgatga agatcttcac gccctcggcc
     4741 ttgaggctgt ggtcgagcag gtgcaggaag tcgcgcttct cgttgaacgc ctcgaacagg
     4801 cgccgcagct tctcgacgct ggtcagctcg gccgcgccca tcaggttggt ctcgcccttg
     4861 atgacgtact cgaggcgctc ctcgccttcc ccggcctcga atacgtgctg ggcgaccgag
     4921 atcgcatcca gcatgatctg actcatgtgg gcgtgcgcct cggcgagctg gcgcaggatc
     4981 tcgtggcgca cctgctgcag ggtgcggccc cggaactgct cgttcagata attcgaggcc
     5041 cgcttgagct catcggggga gtaatagcgc tcgagctgaa tgatgcggtt ctgcacttcg
     5101 cggtcgttga aaacgagc
//
LOCUS       DASRHN010000079         7736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1015303, whole genome shotgun sequence.
ACCESSION   DASRHN010000079 DASRHN010000000
VERSION     DASRHN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015303"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="VFB37_02995"
     CDS             complement(<1..1438)
                     /locus_tag="VFB37_02995"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZO21444.1"
                     /translation="MDKVYAPQEIERRTYQRWEARDSFRPRGTGQPYCIVIPPPNVTG
                     TLHLGHAFQHTLMDALTRYHRMRGDDTLWQPGFDHAGIATQMIVERQLNAQGISRLDL
                     GREAFVERVWEWKNRSGGTMAQQMRRLGDSVDWSRDRFTMDPGLSSAVVEVFVRLHQE
                     GLIYRGKRLVNWDPVLLTALSDLEVQSEQEEGQLWHLRYPLQDRSGYIVVATTRPETM
                     FGDVAVAVHPDDERYALLVGRTLRLPLSERLIPIIADSYVDPAFGSGAVKITPAHDFN
                     DYEIGERHGLPQLNIFTPRAALNDSVPERFRGLDRLEARARVVAELAGAGLIERTEKH
                     LLVVPRGDRTGAVLEPYLTDQWYVRIAPLAAPAIAAVESGRTRFVPESWARTYFEWMR
                     NIKDWCISRQLWWGHRIPAWYDPQGAVYVGRTEDEVRAQHRLPPSLHLRQDEDVLDTW
                     FSSALWPFSTLGWPQQTPALAKFYPTSAL"
     gene            complement(1539..2084)
                     /locus_tag="VFB37_03000"
     CDS             complement(1539..2084)
                     /locus_tag="VFB37_03000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZO21445.1"
                     /translation="MADSAGTSDSADSSAAKRSSPVSSDADRSPGVGSPGAQLRVDFY
                     VLEEASVTARLRLGCKLAEKAYLSAQRALVRLSDAQELGAFDELLWTFMEGSFVPHDV
                     PGASAADEAPVLLSTGAPPPGAFDIIINLAPDIPGGLEQTRRVAEIVDGEETRRRAGR
                     SRFKAYRELGVVPASHNIRAE"
     gene            complement(2103..3596)
                     /locus_tag="VFB37_03005"
     CDS             complement(2103..3596)
                     /locus_tag="VFB37_03005"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZO21446.1"
                     /translation="MHFEVWARGLEALGVECLVVGVFEDGELADQARTLDSVTSGALK
                     KVVSRGDLSGRAGETLLLTDLAGIEASRILLTGLGPQKSFNRKAWRRAVTSALAAVSR
                     TRITSLALAIDRPGARELDDYYFGRSVAELAAASLYRVNDLKSAKKPKPPALQTLLAG
                     PVRKAGIKAAERGLVHGEAVGAAAAVQRDLANLPANVCTPTYLAEQARALAKQYPSLR
                     VQVLDEAAIRREKMGCLLAVAQGSHQAPRFIVLEHKAGKAQSPPVVLVGKGVTFDSGG
                     ISLKDPPGMDEMKFDMSGAAAVIASLSLAARLRLPLRVIGLVAAVENMPSGHAVKPGD
                     IATSASGLTVEILNTDAEGRLILSDALHYSRRFNPAAVVDIATLTGACVVALGHHHAG
                     AMGNDDRLVRELVEAGVRADDRAWHLPLTEEYAEQLKSNFADFANVGGREGGAITAAA
                     FLAKFTQGLKWAHLDVAGTAYQSGAQKGSTGRPTPLLADFLIHRAGV"
     gene            3741..4829
                     /gene="lptF"
                     /locus_tag="VFB37_03010"
     CDS             3741..4829
                     /gene="lptF"
                     /locus_tag="VFB37_03010"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HZO21447.1"
                     /translation="MGKILGRYILREAAVASVVVTGVLLVILLANQVAVVLERAALNQ
                     FPRGVVLELIGLGALQNLSILIPVGLLLGVVLAFGRLYHDSEMASALACGVRPWTLYS
                     PVALLALVASGTLAWLTLSLSPEATARALSLRNTAVRAGQFAPITPGKFRTFGGVNAV
                     VYAQDVKPDGTLGNVFVEHNEGPRVEVAVAERARHTVTPDGMTHTITLYDGERFEGVP
                     GSPQFRIVRFGEHVVPVQVPALSDVVKNIEAQPTSTLLGSRDPDRRAELHWRIALPSM
                     CFVLTLLAIPLARLRPRQGRYSRIWLAVVVYFVYSNLISAGRVWLARGDIPDFLGLWW
                     THLVVVLLCAALVFGPRTLANMRLRRRQ"
     gene            4826..5893
                     /gene="lptG"
                     /locus_tag="VFB37_03015"
     CDS             4826..5893
                     /gene="lptG"
                     /locus_tag="VFB37_03015"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HZO21448.1"
                     /translation="MSLLDRYIVRAILGSVLLVMSVVLVLGALLVFIDQQDDIGVGHY
                     TTLSALWFTLLNLPQQAYELLPITALIGSLLGLGSLARGSELVVVRATGVSVRRIAGA
                     AALAGLLLIGLELLLGEVLAPPLQEAAKEQKAFSKFNNISFGGGSGAWVRDGNLILNV
                     ARQSGQRQFGGMQVFELSPDHRLLAIGHAGRATAEANRSWLLSDYTESRFTPERVLAQ
                     PLGQRVLQSNVTAGFLGLAVEDPGQLTSRALLKLIRYLNANSLNAREYLFAFSSRIAR
                     TVAIMFSVLLAVPFVLGSLRSAGAGTRTMLGLALGIGLFLLQRLIESGTVVFDLDPVV
                     LAWLPTALLAAVTLLLLARAR"
     gene            complement(5870..7033)
                     /locus_tag="VFB37_03020"
     CDS             complement(5870..7033)
                     /locus_tag="VFB37_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HZO21449.1"
                     /translation="MSARRVTILLLLGVLVIAFAIWLSSTRHLEHATLTGDLVLPGLE
                     SAVNTVTEVRLHKGDGTQTTLRKGSSDWIVSERSYPADSSKVRKLLLDLAALNVVEEK
                     TRTPENYPQLGVEDVSSPKATGTQVDAVTPTKTYSLIVGKSSSGKSGYVRVTGRPESL
                     LATPLLSLDPAPQRWLDPAIIDIAQERVKEFSVKPASGPAYTASRDKKEQQDFKVSDV
                     PKGRELSSPTAADPIAGSLAGLTLDDVRRAGQPANGANASSASSSTPGATPTPAATPG
                     EHVTFRTFDGLEIEVSGHKEGTRTFIALTPRSSAKETQAEAANLEARLKGWEFEIPSY
                     KYEGMFRPLEELLKKLPEPAKSTGKQGKGHGGAVIPGLPKKSGAASSSGPGEQ"
     gene            complement(7030..>7736)
                     /locus_tag="VFB37_03025"
     CDS             complement(7030..>7736)
                     /locus_tag="VFB37_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21450.1"
                     /translation="AARITGKVKTMFPSGPPNGVKLATGQTALKESTKPLNLVVFADT
                     DMLSDYLWVREQDFFGQRIQQAWANNGDMVLNTLDNLAGSADLISVRGRASFTRPFDR
                     VEALRRNADDRFRAKEQELEQQLRDTEQKLTQLQSRRNDKSAIILTPEQESELEHFQQ
                     EKLRIRKDLRAVRAGLDEDISHLGTLIKILDIVVFPALFVLIALLIGLWRRQRRRHAS
                     GSSAPAAAQTPREVRS"
BASE COUNT         1200 a         2522 c         2580 g         1434 t
ORIGIN      
        1 tcagcgcgct ggtggggtag aacttcgcga gcgcgggcgt ctgctgcggc catcccagcg
       61 tggagaacgg ccagagagcc gaagagaacc aggtgtcgag aacgtcctcg tcctgacgga
      121 ggtgcaggct gggcggcaag cgatgctgcg cgcgaacctc gtcctcagtg cgaccgacat
      181 agaccgcgcc ctgcgggtcg taccaggccg gaatccgatg tccccaccag agctgtcggc
      241 tgatgcacca gtccttgatg ttgcgcatcc attcgaagta ggtgcgggcc cagctttcag
      301 gcacgaagcg ggtccgaccg ctctcgaccg cggctatggc aggtgcggcg agcggtgcga
      361 tcctgacata ccactgatca gtcaggtacg gctcgagcac tgccccggtg cgatcgccac
      421 gcggtacgac gagcaggtgc ttctcggtcc gctcgatcag accggcgccc gcgagctccg
      481 ccactacgcg ggcccgcgcc tccagtcggt ccagtcccct gaaacgctcg gggacactgt
      541 cattgagcgc cgcgcgggga gtgaagatgt tgagttgggg aagcccgtga cgctcaccga
      601 tctcgtaatc attgaagtcg tgggccggcg tgatcttcac ggcaccggat ccgaatgccg
      661 ggtcaacata ggaatccgcg atgatgggga tcaaacgctc ggacagtggc aggcgcaagg
      721 tccgaccgac caacagcgca taacgctcat catcggggtg cacggcaacg gccacgtcgc
      781 cgaacatggt ctcgggccgg gtcgtagcga cgacgatgta gccggatcga tcctgcagcg
      841 ggtagcgcag atgccacagc tggccttcct cctgctcgct ttgcacttcg agatcggaca
      901 atgcggtgag cagcaccggg tcccagttga ccaggcgctt gcctcggtag atgaggccct
      961 cctggtgcaa tcgcacgaat acctccacca cggcgctcga gagccccgga tccatggtga
     1021 aacgatcacg tgaccagtcc accgagtcac cgagccggcg catctgctga gccatcgtgc
     1081 cgccggagcg gttcttccac tcccagacgc gctccacgaa cgcctcgcga cccagatcga
     1141 ggcgcgagat accctgagcg ttcagctgcc gctccacgat catctgcgtc gcaatgccgg
     1201 cgtggtcgaa gcccggctgc cagagcgtgt catcgccccg catgcggtga taacgcgtca
     1261 gggcatccat caatgtgtgc tgaaaggcgt gcccgagatg caatgtgccg gtaacgttcg
     1321 gcgggggaat cacgatgcaa tagggctggc cggttccgcg cggtcgaaac gagtccctgg
     1381 cctcccagcg ctggtaggtc cgccgctcga tctcttgcgg cgcgtaaact ttgtccatcg
     1441 tgctgctgct ggtgtcctat cggggagagc cgccgggggc gcctcgggac gattatagcg
     1501 atcgggaggc gcctgggccg gcaaggcctt ggacgcagct actcggcgcg aatgttatgc
     1561 gaggcgggga ccacgcccag ttcgcggtaa gccttgaagc ggctgcgccc ggcgcgccgg
     1621 cgcgtctctt cgccgtccac gatctccgcc actcggcggg tctgctccag gccgccgggg
     1681 atatcgggtg cgaggttgat gatgatgtcg aaggcgcccg ggggaggtgc gccggtgctc
     1741 aacagaaccg gcgcctcgtc ggccgccgag gcacccggca cgtcgtgtgg aacgaagctt
     1801 ccctccatga aggtccacag cagctcatcg aatgcaccga gctcctgcgc atcactcagt
     1861 cgcaccaggg cccgttgtgc ggaaaggtag gctttttccg cgagcttgca gccaagccga
     1921 aggcgggcgg tgacggaggc ctcctcgagg acatagaaat cgacacgcaa ctgtgcgccc
     1981 ggactgccga cccccggcga cctgtcagcg tcggatgaga cgggcgacga gcgctttgcg
     2041 gcgctcgaat cggcagaatc ggatgtgccg gcgctgtcgg ccatggctca caccgttgcg
     2101 cctcaaacgc ccgcgcgatg gatcagaaag tccgcgagca gcggcgtggg ccggcccgtg
     2161 cttcctttct gagcaccgga ttgataggcg gtcccggcca cgtcgagatg tgcccatttc
     2221 agcccctggg tgaacttggc aaggaatgcc gcggccgtga tggctccgcc ttcccggccg
     2281 ccgacgttcg cgaagtccgc gaagttgctc ttgagctgtt cggcgtattc ctcggtcaaa
     2341 ggcagatgcc acgcccggtc gtccgcgcgg actccggcct ccacgagctc acggacgagc
     2401 ctgtcgtcgt tgcccatggc gccagcgtga tgatgcccga gagcgacgac gcacgcaccc
     2461 gtcagggtgg cgatatcgac cacggcagcg ggattgaagc gccgcgagta gtgcagggca
     2521 tcgctgagga tcagtcggcc ttccgcatcc gtgttgagaa tctcgactgt caggcccgag
     2581 gcgctcgtgg cgatgtcccc gggcttcacg gcgtgtccgc tcggcatgtt ttccaccgcg
     2641 gcgaccagcc cgatgacccg caggggcaag cgcaaccgag cggccaggga caatgaggcg
     2701 atcacggcgg ccgccccgct catgtcgaat ttcatctcgt ccatgcccgg cggatccttg
     2761 agcgagatgc caccggaatc aaacgtgacg cccttgccga ccagcaccac cggcgggctt
     2821 tgcgccttgc cggccttgtg ctcgagcacg atgaagcggg gcgcctggtg gctgccctgc
     2881 gcgacggcga gcaggcagcc catcttttcg cggcgaatgg cggcctcgtc caggacctga
     2941 acgcgcaacg agggatactg tttcgccagc gcacgagcct gctcggcaag ataggtcggc
     3001 gtgcacacgt tggcgggcag gttggcgaga tcgcgctgca cggccgccgc agcgccgact
     3061 gcctcgccgt gaacgaggcc gcgctcagca gctttgatcc ccgccttgcg cactggaccg
     3121 gccagcagcg tctgcagcgc aggtggcttc ggcttctttg cgctcttcag gtcgttgacg
     3181 cgatacagac tggcggccgc cagctccgcg acgctccgcc cgaagtagta atcgtccagc
     3241 tcccgtgcgc ccggccggtc gatggcgaga gcgagactcg tgatccgggt gcgtgagaca
     3301 gctgccaggg cgctcgtcac ggcgcgccgc caggccttgc ggttgaaact cttttgaggt
     3361 ccgaggccgg tcagcaggat ccgcgaggcc tcgatgcccg ccaggtccgt gagcagcagc
     3421 gtctcgccgg cgcgccccga caggtcaccc cgggagacca ctttcttgag ggctccgctc
     3481 gtgacggagt cgagcgtccg ggcctgatcg gccagctccc catcctcgaa gaccccgacc
     3541 acgaggcact ccacgccgag cgcctccagt cctctggccc agacctcgaa atgcattcga
     3601 tcgccccctc tgattgcgcg cccggaagaa tctgatttac cttacccgcc tcgcctgcgc
     3661 cacgccggcg cttgttgatt cgtgcgtatc gaggcgccgg gcgctggccg gcgccaagtg
     3721 taacctgagc gagtggctgg ttggggaaga tcctcgggcg ctacatctta cgggaagccg
     3781 cagttgcatc ggtggtcgtc acgggtgtgc tgctggtgat cctgctcgcg aaccaggttg
     3841 cggtggtgct cgagcgcgct gcgctcaatc agtttccccg aggggtggtg ctcgagctga
     3901 tcggcctcgg cgccctgcaa aatctcagta ttctcattcc ggtcggcctg ttacttgggg
     3961 tggtccttgc atttgggcgt ctctatcacg acagcgagat ggcgtccgcg ctggcctgtg
     4021 gcgtgaggcc ctggaccctt tactctcccg tggctctgct ggcactggtg gcctccggca
     4081 cgttggcctg gctcacgctt tccctgtcgc cagaggcgac ggcacgcgca ctcagcttgc
     4141 gcaataccgc cgtgcgtgcc ggccagtttg cgccgatcac tccgggcaaa ttccgcacgt
     4201 tcggcggggt gaatgccgtc gtctacgcgc aggatgtcaa acccgacgga accctgggca
     4261 atgtgtttgt cgagcacaac gaggggcctc gggtcgaagt tgccgtcgcc gagcgcgcgc
     4321 ggcacaccgt cacgccggac ggcatgacac acacgatcac gctgtatgac ggcgagcgct
     4381 tcgagggcgt tccggggagc ccgcagtttc gtatcgtgcg cttcggtgag cacgtcgttc
     4441 cggtccaggt ccccgccctg tccgatgtgg tgaaaaacat cgaggcacag cccaccagca
     4501 cgctgctcgg ctcgcgcgat ccggatcggc gcgccgagct gcattggcga atcgcgctac
     4561 cctcgatgtg cttcgtgctg acgctcctcg ccattccgct cgcgcggctg cggccgcggc
     4621 aagggcgcta ttcgcgcatc tggctcgcgg tcgtggtcta cttcgtctac tcgaatctca
     4681 tctcggcggg tcgcgtgtgg ctggcgcgcg gagacatacc cgacttcctc ggtctatggt
     4741 ggacccatct ggtggtggtg ctgctgtgcg ctgcgttggt gtttgggccg cgtacactgg
     4801 ctaacatgcg tctgaggcgc cggcaatgag tctgctcgac cgctacatcg tgcgggcgat
     4861 cctcggctcc gtgctgctcg tcatgtcagt ggtgctggtg ctcggcgcgc tgctggtatt
     4921 catcgatcag caggacgaca tcggtgttgg ccactacacc acgctcagcg cactctggtt
     4981 cacgctgctc aatcttcccc agcaggctta cgagctgctg ccgatcacag ctctcatcgg
     5041 ctcgctgctc ggcctcggct cgctggctcg cggcagcgag ctcgtggtcg tgcgcgccac
     5101 aggtgtgtcc gtcaggcgca tagccggcgc tgcggccctg gcgggattgc tgctgatcgg
     5161 gctggagctg cttctgggag aggtgctggc gccgccgctg caggaagcgg ccaaggaaca
     5221 gaaggccttc agcaaattca acaacatcag cttcggcggg ggcagcggtg cctgggtgcg
     5281 cgacggcaac ctgatcctca acgtggcgcg ccagtcgggg caacgtcagt tcggtggcat
     5341 gcaggtgttc gagctgtccc ccgatcaccg tctgctcgct atcggccatg cggggcgcgc
     5401 cacggccgaa gcgaatcgca gctggctgct cagcgactac accgaatcga ggttcacgcc
     5461 ggagcgggtg ctggcacagc cgctcgggca gcgagtgctg caatccaacg tgacggccgg
     5521 ctttctcggg cttgcagtcg aagacccggg ccagctcacc agccgcgccc tgttgaagct
     5581 gatccgctat ctgaatgcca actccctcaa tgcgcgcgag tatctgtttg cattctcctc
     5641 gcgcatcgcg cgcacggttg ccatcatgtt ctcggtgctg ctcgccgtgc ccttcgtgct
     5701 cgggtcgctg cgctctgccg gagcggggac gcggacgatg ctcggcctgg cgctcgggat
     5761 cggcctgttc ctgctgcagc gactcatcga gagcggcacg gtcgtctttg acctcgatcc
     5821 ggtggtgctc gcctggctgc cgacggcgct gcttgccgcc gtcacactgc tactgctcgc
     5881 ccgggcccga tgaggacgca gcgccggact tcttgggcag gccggggatt acggcgccgc
     5941 cgtggccctt gccctgcttg ccggtgctct tggcgggctc cggaagcttc ttcagcagct
     6001 cctccagcgg cctgaacatg ccttcatact tgtagctggg gatttcgaat tcccagcctt
     6061 tgaggcgcgc ctcgaggttc gcagcctccg cctgggtctc cttggcgctc gagcgcggcg
     6121 tcagcgcgat gaaggtccgc gtcccttcct tatggccgga cacttcgatt tccagaccgt
     6181 cgaaggtgcg gaaggtgacg tgctcgcccg gcgtggcggc aggcgtgggc gtcgccccgg
     6241 gtgtggaaga gctcgcactg gaggcgttcg cgccatttgc cggctgtccg gcgcgccgga
     6301 cgtcatcgag cgtcaatccg gcgagcgatc ctgcaatcgg atcggcggct gtcgggctgc
     6361 tcagctcgcg acccttggga acatccgaca ccttgaagtc ctgctgctcc ttcttgtcgc
     6421 ggctggcggt gtaagccggg ccgctggcgg gcttcaccga gaactccttc acgcgctcct
     6481 gcgcgatgtc gatgatcgcg gggtcgagcc agcgttgcgg agcggggtcc agcgacaaga
     6541 gtggagtggc gagcaggctc tcgggacggc ccgtgacgcg cacgtagccg gatttgccgc
     6601 tggagctctt gcccacgatg agcgagtaag tcttggtggg cgtcaccgca tccacctggg
     6661 tgccggtcgc cttgggcgag ctcacgtcct cgacgcccag ctgcggatag ttttccggtg
     6721 tgcgtgtctt ctcctcgacc acgttgagcg cggcaaggtc cagaagcagc ttgcggacct
     6781 tgctcgaatc ggccggatag ctgcgttcgc tgacaatcca gtcggaggat cctttgcgca
     6841 gggtggtctg agttccgtca cccttgtgca gtctgacctc ggttaccgtg ttgacggcac
     6901 tctccagccc cggcagaacg agatcaccgg tgagggtcgc gtgttccagg tggcgcgtcg
     6961 aggacagcca gatcgcaaac gcgatgacca gcacgccgag gagcagcaga atcgtcactc
     7021 gccgggcact catgagcgga cttccctggg agtctgtgcc gctgccggcg cggatgagcc
     7081 gctggcgtgg cggcgccgct gtctgcgcca caggccgatg agcaacgcga tgaggacgaa
     7141 cagggcgggg aagaccacga tgtccaggat cttgatcagc gttccgagat ggctgatgtc
     7201 ctcatcgagg ccggcgcgca ccgcgcgcag atccttgcga atgcgcagct tctcctgctg
     7261 gaagtgctcg agctcgctct cctgctcggg cgtcaggatg atggccgact tgtcattgcg
     7321 tcgtgactgc agctgcgtga gcttctgctc cgtgtcacgc agctgctgct cgagctcctg
     7381 ctccttggcg cggaagcggt cgtccgcatt gcgacgcagg gcctcgaccc ggtcgaaagg
     7441 ccgggtgaag ctcgcgcggc cgcgcacact gatgaggtcg gcgctgccgg ccagattgtc
     7501 cagcgtgttg aggaccatgt cgccattgtt ggcccaggcc tgttggatac gctgcccgaa
     7561 gaaatcctgc tcgcgcaccc acagatagtc ggacagcatg tcggtatcgg cgaacaccac
     7621 gaggttgagc ggcttggtgg attccttgag cgcggtttgc cccgtggcga gcttgacgcc
     7681 gttcggcggg ccgctgggga acatcgtctt caccttgccg gtgatgcgcg ccgcga
//
LOCUS       DASRHN010000080         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_519645, whole genome shotgun sequence.
ACCESSION   DASRHN010000080 DASRHN010000000
VERSION     DASRHN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519645"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFB37_03030"
     CDS             complement(<1..368)
                     /locus_tag="VFB37_03030"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HZO21451.1"
                     /translation="MEPEPLRINIWPNCSLSVRAAFLFFGSTCFVSFTIAGVMASRGF
                     WPVLPFAGLEMLLLGWALKTSLDRRHHRQTITVTDASVSIELKDGAHSVQLVFPRHWA
                     QVKLRRPDSRLHPSRLTIES"
     gene            complement(399..1112)
                     /gene="bioD"
                     /locus_tag="VFB37_03035"
     CDS             complement(399..1112)
                     /gene="bioD"
                     /locus_tag="VFB37_03035"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZO21452.1"
                     /translation="MSHTPRGLFITGTDTEVGKTLVAVSLVRALVKGGLRVAVMKPVA
                     SGAEPMPLGLRNSDALALLRAANVPAPYERVNPYCYVPPISPHLAAKEAAFPVDLRVI
                     EECFDALRGNADLVVVEGAGGWYAPISEKHTMQDLALRLDLPVVLVVAMRLGCLNHAM
                     LSRRAIEAAGANLAGWVANELDPGFERRAENLESLVQLMGSPPLAVLPFQPDEATVLL
                     AAEEAVRALRLSQPRKRLT"
     gene            complement(1118..1996)
                     /gene="bioC"
                     /locus_tag="VFB37_03040"
     CDS             complement(1118..1996)
                     /gene="bioC"
                     /locus_tag="VFB37_03040"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HZO21453.1"
                     /translation="MSADEFSLDRAGIRTAFDRASARYEASAGLQARIGDELLGRLEL
                     FRFQPQTVLDLGSGPGRASEELKRRYRHALVVALDLAPGMLREAGRRQRLFRRFERVC
                     GDAMRLPLADASVDVVYSSLMLQWCEPIDVVFAEIRRILRPAGFFAFSTFGPDTLQEL
                     RAAWSSADGHNHINHFADMQQVGEALMRAGLSEPVLDVDRIELTYADTLTLMRDLKAI
                     GAHNVTAGRPRGLTGRASLQRMQLAYEAFRRDGGLPATYEVIYGATWGASGQPTAAFG
                     AEVHIAPSSIRRRRFR"
     gene            complement(1993..2928)
                     /locus_tag="VFB37_03045"
     CDS             complement(1993..2928)
                     /locus_tag="VFB37_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO21454.1"
                     /translation="MTALHVEVSGTGPDLVLLHGWGVNLRVWDGLVKALCGRFRVITL
                     DLPGHGRSPSGPAGTPAERSWLVHQTLSGLTNHYSLLGWSLGGQIALDLAAAVPGQIG
                     RIVLVGTTAKFVAAPDWPHGMPAAAMAALATKLRADCQRTVHDFIDLQARGSARGDHV
                     RVQLHSALSIHGEADAQALDRALESLAQSDLRAALGHVRSPALVVSGQYDRITPPAAS
                     RALARALPDARYVELRRASHAPFLSHESTFCRLLERFLRPRAARTQRKAKAHKAARPK
                     KPLRPAPKAGRRLSRMSAVKRKVRRRTRRARARGR"
     gene            complement(2925..4133)
                     /gene="bioF"
                     /locus_tag="VFB37_03050"
     CDS             complement(2925..4133)
                     /gene="bioF"
                     /locus_tag="VFB37_03050"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HZO21455.1"
                     /translation="MKRGPSEVEAALAQIDRDSLRRERRTLEEFVEPDSRVTVRAAGR
                     LLVDFSSNDYLGLARHPEVVSAMSDCATRCGAGSGASHLVTGHGAEHARLEEELAAFT
                     GRERALLFSTGYMANLAVISTLAGRGEWVLLDRLSHASLIDAGLLSGARFRRYPHLDA
                     TAAETQLTGLSQAAAVVATDGVFSMDGDLAPLDALARSTRKHEAWLVVDDAHGLGVLG
                     KTGRGSLEHYGLDAESVPVLVGTLGKALGSFGAFVAGSSDLIELLMQRARAYIYTTAL
                     PQPVAAATRKALQIAQRESWRRERVLALTARFRAAAVQLGVPLLPEGAQPLTPIQPIV
                     LGGSKAALAAQQELLVRGFCVIAIRPPTVPRGTARLRVTLSAAHSESQIDALAEALSI
                     ACTQQRRNGA"
     gene            complement(4114..>4652)
                     /gene="bioB"
                     /locus_tag="VFB37_03055"
     CDS             complement(4114..>4652)
                     /gene="bioB"
                     /locus_tag="VFB37_03055"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HZO21456.1"
                     /translation="EAGLDFYNHNLDTSPEFYDEIITTRTYQDRLDTLGAVREAGLKV
                     CCGGIIGMGESITDRAQLLRTLANLPEHPESVPINQLVQVPGTPLYGRDGVDPFDFVR
                     TIAVARLLMPAAHVRLSAGREAMTDETQALCFFAGANSIFYGEKLLTTGNPDVERDQR
                     LFARLGLQAESASEARAI"
BASE COUNT          796 a         1580 c         1526 g          750 t
ORIGIN      
        1 tgcgactcga tcgtgagccg gctcgggtgc aagcgcgaat cagggcggcg aagtttaacc
       61 tgcgcccaat gccgcgggaa cacgagttga acggaatgcg ctccgtcctt cagctcgatg
      121 ctgacactgg catcggtgac ggtgattgtc tgtcggtgat ggcgccgatc caggctcgtc
      181 ttcagagccc agccgaggag cagcatctcc agccctgcga agggcagcac gggccagaag
      241 ccacggctgg ccatgacgcc tgcgatcgtg aaggagacga aacaggtgct accgaagaac
      301 aggaacgctg cacgcacgct gagcgagcag ttcggccaga tgttgatacg caacggctcg
      361 ggttccactt tgggatacta ctgcgcggga atttttattc aagtcaagcg cttacgcggt
      421 tgcgagagcc tcagcgcgcg cactgcctcc tctgccgcca gcaataccgt cgcctcatcc
      481 ggctgaaacg ggagcacggc cagtgggggc gatcccatga gctggacgag cgattcgaga
      541 ttctccgcgc gtcgctcgaa tcccgggtcc agctcattcg ccacccaacc cgcaagattc
      601 gcgccggccg cttcgatcgc gcgtcggctg agcatcgcat ggttcagaca acccaggcgc
      661 atggcgacga ccagcacgac gggaaggtcg agcctgagag ccaggtcctg catcgtatgc
      721 ttctcgctga tgggcgcgta ccagccgccc gcgccttcca cgaccacgag gtccgcgttg
      781 cctcgaagtg cgtcgaagca ttcctcgatc acgcgaagat ccaccgggaa agcagcctct
      841 tttgcggcga gatgcggcga aatcggcgga acgtagcaat atgggttgac ccgctcgtaa
      901 ggcgccggca cgttcgcggc gcgcagcagc gccagggcat ccgaattgcg aagcccgagc
      961 ggcataggtt cggctcctga agcaaccggc ttcatgaccg cgacccgcaa gccgcctttc
     1021 acgagagctc gcaccagcga caccgcgacg agcgtcttgc cgacttcggt atcggttccg
     1081 gtgatgaaaa gaccccgcgg tgtgtgactc acgggcttca ccggaagcgg cggcgacgaa
     1141 tggagctcgg cgcgatgtgc acttcggcgc cgaacgccgc ggtgggctgg cccgaagctc
     1201 cccaagtggc cccgtaaatg acctcgtatg tagcgggcaa tccgccgtcg cgcctgaagg
     1261 cttcgtaagc gagctgcatg cgctgcaggc tggcgcgccc cgtcaggccg cggggccgcc
     1321 cggcggtcac gttgtgagcg ccaatcgcct tgagatctct catcagcgtg agcgtgtcgg
     1381 cataggtcag ctcgatgcga tccacgtcca acacgggctc gctcaggccg gcccgcatga
     1441 gcgcctcccc gacctgctgc atgtcggcga agtggttgat gtggttatgg ccatcggcgc
     1501 tcgaccaggc ggcacgcagc tcctgcagcg tgtctggccc gaacgtgctg aaggcaaaga
     1561 agcctgcggg ccgcaggatc ctgcggatct cggcgaacac gacgtcgatg ggctcacacc
     1621 actgcagcat gaggctgctg tagacgacat ccacgctggc gtccgccagg ggcagtcgca
     1681 tggcatcgcc gcacacgcgc tcgaaccggc ggaacagccg ctgacgccgc ccggcctcgc
     1741 gcagcattcc cggtgcgagg tcgagcgcaa cgaccagcgc atgccggtag cggcgcttga
     1801 gttcctcgct cgccctgccc ggacccgagc ccaagtcgag aacagtctgc ggctgaaagc
     1861 ggaacagctc caggcggccg agcagctcat ctccgattcg cgcctgcagc cccgcgctcg
     1921 cctcgtatcg cgcgctcgcg cgatcgaaag cggtgcgaat gcctgcgcga tcgaggctga
     1981 actcatcggc actcatcgac ctcgggccct cgctcgccgg gtccgtcgtc gcaccttgcg
     2041 cttgactgcg ctcattcggg aaagccgccg tcccgctttc ggggccggcc gcaggggctt
     2101 cttgggtcga gccgccttgt gcgccttggc tttgcgctgg gtccgcgctg ctcgcgggcg
     2161 caggaatctt tcaagcagcc ggcaaaatgt gctttcgtgc gacaggaacg gcgcgtggga
     2221 ggctcgtcgc agctcgacgt agcgcgcatc cggcagcgcc cgggccaatg cacgcgaagc
     2281 tgccggcggc gtgatgcgat cgtactggcc ggacaccacc agggccggcg agcgcacgtg
     2341 ccccagagcg gcccgcagat cgctctgcgc cagcgactcg agcgcgcgat cgagtgcttg
     2401 cgcatccgcc tcgccatgta tcgaaagtgc actgtgaagt tgcacgcgga cgtgatcccc
     2461 acgcgcactg ccgcgcgcct gcaggtcgat gaagtcgtgc acggtgcgct ggcaatccgc
     2521 tcgcaatttc gtggccagag cggccatcgc cgcggccggc atgccgtggg gccagtcggg
     2581 tgcggcgacg aactttgccg tggttccgac cagcacgatg cgcccgatct gtcccggtac
     2641 ggcggcggcc agatcgagcg caatctgtcc acccaatgac cagccgagaa gtgagtaatg
     2701 attggtgagc ccgctgaggg tctggtggac gagccaggag cgctcggccg gtgtaccggc
     2761 cggcccgctc ggactgcgac catggcccgg caagtccagg gtgatgaccc gaaatcggcc
     2821 gcagagcgcc ttcaccaacc catcccacac gcgcaggttc accccccatc catgcagcag
     2881 cacgagatct gggccggtgc cgctcacctc gacatgcagc gccgtcatgc cccgttccgg
     2941 cgttgttgcg tgcaggcaat gctcagcgcc tccgcgagcg catcgatctg cgactccgaa
     3001 tgcgctgccg agagggtcac tcgcagccgg gcggtgccgc ggggcacggt cggtggccgg
     3061 atcgcgatga cacagaagcc gcggacgagc agctcctgtt gagccgcgag agccgccttc
     3121 gagcccccca gcacgatggg ttggatgggc gtcagcggct gggccccttc aggcagcagt
     3181 ggaacgccga gctgcaccgc cgccgcgcgg aatctcgccg tgagcgccag aacccgttct
     3241 cgccgccagc tctcgcgctg tgcaatctgc agtgccttgc gagttgcggc ggcgactggc
     3301 tggggcagtg cagtggtgta gatgtacgcg cgcgctcttt gcatcagcag ctctatcagg
     3361 tcgctcgagc ccgcgacgaa cgcaccgaag gagcccagtg ccttgccgag cgtgccgacc
     3421 agcaccggaa ccgactcggc atcgaggccg tagtgctcga gcgaaccgcg tcctgtctta
     3481 ccgagcacgc ccagcccatg ggcgtcgtcc accaccagcc aggcttcgtg cttgcgagtt
     3541 gaccgcgcca gcgcatcgag cggtgcgagg tctccatcca tgctgaagac cccgtcggtc
     3601 gcaacgaccg ccgcagcctg cgacaacccc gtgagctggg tttccgcggc cgtggcatcc
     3661 agatgcggat agcgcctgaa acgggcccct gacaacaggc cggcgtcgat cagcgaggca
     3721 tggctgagcc gatccaggag cacccactct ccccggccgg cgagcgtgga aatgactgcg
     3781 agattcgcca tgtagccggt ggagaacagc agtgctcgct cgcgtcccgt gaatgcggcg
     3841 agctcctctt cgaggcgggc atgctcggct ccgtgtccgg tgacgaggtg ggaggcgccg
     3901 cttccggcac cgcaacgcgt cgcgcaatcg ctcattgcgc tgaccacctc cggatgccgc
     3961 gccagaccga gataatcgtt gctgctgaag tccacgagca gccggccggc ggccctgacg
     4021 gtcacgcgac tgtcgggctc gacgaattcc tcgagcgtgc ggcgctcgcg tctcagcgag
     4081 tcccggtcga tctgcgcgag cgcggcttcg acctcagatg gcccgcgctt cacttgcgct
     4141 ctccgcctgc aacccgaggc gcgcaaacag cctctggtca cgctccacgt cgggattgcc
     4201 ggtggtcagc agcttctcgc catagaagat cgaattcgcg ccggcgaaga agcacagcgc
     4261 ctgcgtctca tcggtcatgg cttcgcgtcc ggccgacagg cgcacgtggg cggccggcat
     4321 cagcagcctc gcgacggcga tcgtgcgcac gaagtcgaac gggtccactc cgtcccgccc
     4381 gtacagcggc gtgcccggca cctgcacgag ctggttgatc ggcacgctct ccggatgctc
     4441 cggcaggttg gcgagggtgc gcagcaactg ggcacgatcg gtgatcgact cccccatccc
     4501 gatgatgccg ccacaacaga ccttgagccc cgcctcacgc accgccccga gcgtgtcgag
     4561 ccggtcctga taggtgcgag tggtgatgat ctcgtcgtag aactccggcg aggtgtccag
     4621 attgtggttg tagaagtcca gacccgcttc gc
//
LOCUS       DASRHN010000081        15161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_192419, whole genome shotgun sequence.
ACCESSION   DASRHN010000081 DASRHN010000000
VERSION     DASRHN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192419"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFB37_03060"
     CDS             complement(<1..190)
                     /locus_tag="VFB37_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21457.1"
                     /translation="MPERTRWAPGSAALAEAARAVAAVAFDGNSSDEALRPHEDAREG
                     PAVRAITLGSLRWFLRLAP"
     gene            complement(183..1166)
                     /gene="fmt"
                     /locus_tag="VFB37_03065"
     CDS             complement(183..1166)
                     /gene="fmt"
                     /locus_tag="VFB37_03065"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZO21458.1"
                     /translation="MSSLRIAFAGTPDFALRALLALLDSHHHVVGVLTRPDRPRGRGR
                     QLAGSPVKTLAMERGLPLSQPATLTGADDRADLAAWAPDVLVVAAYGLILPSAVLELP
                     SLGCLNIHASLLPRWRGAAPIQRALLAGDAETGISIMRMDAGLDTGPILLQRAIAISA
                     AETGGTLHDRLATLGASALLEALEAVAAGAADAMPQRGQGATYAAKIDKAEARIDWRR
                     DALGIERQVRAFNPWPIAETSLEGAQLRIFSARALGIDACGGAPSVDPAAVVAGAGGE
                     PGTIIDVQDEALYVACGRGVLAIAEVQRPGRRPVSARDFAHTLALKGRRLA"
     gene            complement(1178..1723)
                     /gene="def"
                     /locus_tag="VFB37_03070"
     CDS             complement(1178..1723)
                     /gene="def"
                     /locus_tag="VFB37_03070"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZO21459.1"
                     /translation="MATRTILEFPDPRLRTRAQPVAHFDAGLKQLVEDMFESMYAAPG
                     IGLAATQVDVHQRVIVIDISKERTEPLVFINPEILTREGKAESEEGCLSVPGIFEDVE
                     RAAKVRVRAQDVTGQTFERDCDEVLAVCIQHEMDHLEGKLFVDYLSDLKRQRIRKKLE
                     KERKERAQKSSEGSTRPKRMY"
     gene            2172..3260
                     /locus_tag="VFB37_03075"
     CDS             2172..3260
                     /locus_tag="VFB37_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZO21460.1"
                     /translation="MDELTATEAAASGAGASATAASAPSSADASSIVNPSAPKSYTVK
                     RGDTLWGIASMFLKDPWLWPEVWIINPKVANPHLIYPGDVLALAYGADGRPQIRLDQA
                     GAARLEPRLRSSPLDAAIPTIPYSAIAAFLSRPTVLSSEQVRDAAHIVAFRDEHVVAG
                     TGHDVYISGLNAQPNGRYSVVHIADELRDPDDNKVVGYEGVYTATALVANPGRPTKAL
                     LIDAARETLQGDKVLAADLDVPLNFMLRAPRNEVNGRIISVIDGTQVIGQYQIVVINR
                     GKRHGIDAGHVLAIDQAGEVVHDVYGGATGLSRYLGQAGSSLSPKVRLPDERAGTLLV
                     FKSFDRVSYALIIGASNSIHVGDIVHNP"
     gene            3419..4510
                     /gene="dprA"
                     /locus_tag="VFB37_03080"
     CDS             3419..4510
                     /gene="dprA"
                     /locus_tag="VFB37_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZO21461.1"
                     /translation="MDAVTARVVLARAPGLTAAHVHALMAAVDSDIERVLDAGSLARI
                     ELPAHTRAFLEQPDRSAVLQDCHWLAASGAQLLLSTDALYPLQLAGAPDPPAALFVLG
                     DPRTLREPQLAMVGSRNPTPCGRRTAREFAASFARAGLTITSGLAIGIDAACHEGALQ
                     GGGKTIAVCATGLERVYPAQHGGLAERIRERGALVSEFAPSTALRRANFPRRNRLISG
                     LAVGTLVVEAAQRSGSLITARHAADQGREVFAIPGSIYSPLSRGCHKLIRDGATLVQE
                     PAELLAELRIPLKEEALVEGSRPSGAGSALDKGFEMLLDAVDFEPATVDVLAARTGLA
                     GEVITPMLLMLELEGRIASCAGGRYGRLP"
     gene            4545..5030
                     /locus_tag="VFB37_03085"
     CDS             4545..5030
                     /locus_tag="VFB37_03085"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HZO21462.1"
                     /translation="MTGTVLDILIYVFDRYMLDEVPEVPERAHLARDLERVGFTHANV
                     ERALDWLAELACERDRPLLPLSGENDGPAVRIFSDGELARLSTECRGFLVTLERLGIL
                     SPQQREIVIDRMLALDADELDTEQLKWVVLMVLSSQPGQEQAFARLEDLVSDVHPGTP
                     H"
     gene            5091..7913
                     /locus_tag="VFB37_03090"
     CDS             5091..7913
                     /locus_tag="VFB37_03090"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HZO21463.1"
                     /translation="MPENLLIVESPAKAKTLKKYLGKDFEVLASYGHVRDLIPKEGAV
                     DPDRGFSMQYQVLEKNERHVEAISRSLRKSKALYLATDPDREGEAIAWHLKEILQSRG
                     ELDGKRVHRVAFYEITRNAIRAAVGEPRDLSLDLVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRMICEREAQIAAFIAQEYWTIEGEGSHTGQAFPLKLLEYSGR
                     KVEQFSFVNEAQAREVERTIEAAARSTAQAAAPGLGELRIIAIDRKQRRRNPAPPFTT
                     STLQQEAARKLGFNARRTMRLAQQLYEGIDIGDGSGSVGLISYMRTDSVTLAAEAITE
                     IREVAAREYGKAEVAEEPRIYKTKSKNAQEAHEAIRPTSASILPGPLEGKIDPDLYKL
                     YSLIWKRAVASQMSHAIFDTVAVDMLAGKDGPERHLLRANGSTLVKPGYIAVYQEGTD
                     DSRVDDTDHALPPMEVGDIVTLAALRAEQHFTEPPPRYSEASLVKALEEHGIGRPSTY
                     ATIISTLQDREYVEMDARRFVPTDIGKIVNRFLTDHFHHYVEYGFTAAMEDELDAVSR
                     GEEDWTTPLGKFWKPFIIQVQKIEKNVTREQVAQARELGKDPASGKPMTVRMGRYGPF
                     VQIGTKDDEEKPRFAGLRPGQKMDTLTLADALELFKLPRTLGATADGEPIITNVGRFG
                     PYVKYGSKYVSLKEDDPYTVSLERALEVIRLKQEADANRIIADFSAEGIQVLNGRYGP
                     YVTDGTKNAKIPKDRDPKSLTPADCRELLAQAPARGGRFGRGRRGTQSRPRAGNGQGT
                     GNGSESQPSPTPSDSRPKRPRASTAQSAATGTDKQQKASNRDRSRTTPEAADRKSGTG
                     KGAAGSLAGARSAVTAKLGAAAKAGAKPGPAAKNTAAHARAPGSNPRVGRGSPANKPA
                     RQSPAGKPPAARKNGKSAANRPRGLK"
     gene            7943..8776
                     /gene="rlmJ"
                     /locus_tag="VFB37_03095"
     CDS             7943..8776
                     /gene="rlmJ"
                     /locus_tag="VFB37_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZO21464.1"
                     /translation="MTKYRHSHHAGNFADVHKHVTLLALLASMKRKDKGFGYFETHAG
                     RGAYDKSAASSELAAGAGRFMTSQHTAPELREFASLLTQFRLSRPQHAYPGSPVIASR
                     ELRAQDRAVLCELVPSEARALERELAGNARLRIEQGDGFDRLRAWMPPAERRGLVLLD
                     PAYEDSQDFDRVSASLVDALRRFGTGVFMVWYPIKDEREIRTWQSRMAPMLGREALLS
                     ELWLYPRDSKVALNGSGILIVNPPWLLAERMHRWLPELQALLDTGHEGGTSVTKLTST
                     A"
     gene            8808..9680
                     /gene="aroE"
                     /locus_tag="VFB37_03100"
     CDS             8808..9680
                     /gene="aroE"
                     /locus_tag="VFB37_03100"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZO21465.1"
                     /translation="METQKDRYGVVGHPVGHSWSPFIHGLFARDTAQAMSYRLYDFRP
                     KEFEPRVRDFFTLGGRGLNVTLPHKGAAVKIASELTSRATDAQAVNTLAAQKDGSILG
                     DNTDGLGLVRDLCDNLGLVVTRRRLLILGAGGATRGVLGPLLTLEPSEIVIANRTTER
                     ASSLAEAFATLGPVRGAGLAEIPGGPYDLIINATSAGLSGEIPAVGAGIIGPETTCYD
                     MSYGKSDTPFTKWALDLGCARAFQGWGMLVEQAAESFRVWRGMRPSTASVLSALKERA
                     LSASQATAGALSAS"
     gene            complement(9667..11022)
                     /gene="gor"
                     /locus_tag="VFB37_03105"
     CDS             complement(9667..11022)
                     /gene="gor"
                     /locus_tag="VFB37_03105"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF004776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HZO21466.1"
                     /translation="MTDQFDLVVIGGGSGGLAAAQRASEYGARVALVESGRLGGTCVN
                     VGCVPKKIMWNAAMIADCLADASEYGFRLGAAEPHDWALLKSKRDAYVARLNGIYESN
                     LAKRGVELLRGRGTFLDAHTVEASGRKLAATHVLIATGGRPLLPAIPGMELGITSDGF
                     FELPQLPERVAVVGSGYIAIELTGILAALGARATLVLRGQAALKEFDSMLGEATLTIM
                     REAGMEVVTNAWPQSLERTVTGALDLEVRDGRRLTPFDTVIWAIGRVPEVEELGLERA
                     EVALDDYGFIVTDKFQVTTSPGVYAVGDVTGRKQLTPVAIAAGRRLADRLFGGQPERY
                     LDYDYIPTVVFGQSPVGTVGLTEDAARAQYGDEAVTVFRSGFIPMYHALTSRKPRTDM
                     KVVTVGAEQRVVGVHVIGPGADEMMQGFAVAVRMGATKRDFDDTVAIHPTSAEELVTM
                     R"
     gene            11155..13191
                     /locus_tag="VFB37_03110"
     CDS             11155..13191
                     /locus_tag="VFB37_03110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZO21467.1"
                     /translation="MENPLLGLEPLPAFARIRPEHVEPGVRALLESSRARLEELVAVE
                     NPTFATVIEPLEELHHRVTRTWSPVSHLNAVLNSEALRTGYNACLPLLSAYQTDLAQS
                     EPLYRAYRAIAEQEGAALGPVQRQLIEHALRDFRLAGVGLDTARKQRFKAVMLELTQL
                     QAKFEENVLDATNGWSCHLTEESELTGLNSTLIEQARRRAHERGMEGWILILDQPTYV
                     AVVTDAESEALRRSFYEAWTTRASDQGPNAGRWDNSQVMEAILRLRHEAAQLLQFRNY
                     AEYALSTRMARSVEEVMQFLVELARSARDAAHREFAELEAFAGRKLAAWDVGFHAERL
                     QRSRYSVSQEELRPYFPLPRVLAGLFEVGQRLFGLRIHERHGAPVWHPDVRFFEIESA
                     AGQLVGSFYLDAYARPSKRSGAWMDECVGRKRLPSGSALPVAYLVCNFLPPSEDHPAL
                     LTHDDVLTLFHEFGHGLHHLLTRVDYPSIAGINGVSWDAVELPSQFLENYAWHPEVLQ
                     RISSHIETGAPLPAETQSRLIATRSFHAGLQMMRQLEFALFDFRLHAEYSPERGARVL
                     EVLEQVRREVAVVPAPPWNRFPNSFGHIFAGGYAAGYYSYKWAEVLAADAFAAFEESG
                     VFDMLTAHRFLDAILTRGGSRDALEAFIEFRGRKPDVRALLKQHGILTESPPAA"
     gene            13422..14435
                     /locus_tag="VFB37_03115"
     CDS             13422..14435
                     /locus_tag="VFB37_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO21468.1"
                     /translation="MKSVRRSDFDVTNTVQVSSPPAVLAEVRSLFEPTWPTLSFEPLD
                     RAFRHFERLFAGDVPGYCGVDTVYHDRQHTLDITLALARLLVGYERQHGEAERLGGSR
                     ALIGLITGLFHDVGYLRRLDDLSIRNGAEFTRTHVSRGARFLQDYLPELGLADAVPLA
                     CEIIHFTGYEVPFSQIKVTDPRDIAVGHLLGTADMIAQMADRCYLEKCRDRLYAEFVL
                     GGVALPIAANGGREVKYASGLDLLRQTPEFIAEVRAKRLDGEFHSAYRSLEILYGGRN
                     PYIEAIDRNVDYLRQILRSENWRLLRRNPPIFAATDDPMSTMRGLMLGYIKRVWASKY
                     EES"
     gene            complement(14475..>15161)
                     /locus_tag="VFB37_03120"
     CDS             complement(14475..>15161)
                     /locus_tag="VFB37_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZO21469.1"
                     /translation="RMLTALELTGRAATHVQELAELSCRLQPDAGRALLRMREAAREA
                     GIDVGIASAFRDFERQLGIWSAKFRGERRLLDRQGNEIAHASLDEPALIEAILIWSAL
                     PGASRHHWGTDIDVIDLASLPAGGRPQLVPHEFAPGGCFAHLDRWLTENMGRFGFFRP
                     YTIDRGGVQPEPWHLSYGPVAVPALDALSLDILAQTIAATDMPGQAQVLERLPEIYER
                     YVCAVEAPVS"
BASE COUNT         2634 a         5029 c         4946 g         2552 t
ORIGIN      
        1 ccggagcgag tctgagaaac cacctcagtg agccgagcgt gatggcgcgg acagcggggc
       61 cttcgcgggc gtcctcgtgg ggccgcaagg cctcgtcgct ggagttgccg tcaaacgcga
      121 ccgccgccac cgcacgggca gcttctgcga gcgcagccga acccggcgcc cagcgcgttc
      181 tttcaggcaa ggcgccggcc cttgagcgcg agggtgtggg cgaagtcgcg agcggacacc
      241 ggacggcgcc ccgggcgctg gacttcggcg atcgccagca cgccacggcc gcaagctacg
      301 taaagcgcct cgtcctgcac gtcgatgatc gtgccaggct cgcctccggc gccggccaca
      361 accgcagccg ggtccacact cggagcacct ccgcacgcat cgatccccaa agcgcgagct
      421 gagaaaattc tcagttgcgc gccctcgaga ctcgtctcgg cgatcggcca gggattgaag
      481 gcacggacct gtctctcgat gccgagcgca tcgcgccgcc aatcgatgcg tgcctccgcc
      541 ttgtcgatct tggcggcgta ggtcgcgcct tgcccgcgct gcggcatcgc gtctgcggcg
      601 ccggcggcca cggcttcgag tgcttcgagc agtgccgagg cgccgagcgt cgccagccgg
      661 tcgtgcagcg tgccgcccgt ctccgcggcc gagatcgcga tggctcgctg caagaggatc
      721 ggacccgtat cgagcccggc atccatgcgc atgatgctga tgccggtctc ggcgtctcct
      781 gccagcagcg cgcgctgaat cggtgcggca ccgcgccagc gcggcaacag cgaggcatgg
      841 atattgaggc agcccagcga aggcagctcg agcacggcag agggcagaat aagaccgtag
      901 gctgcgacaa ccagaacatc gggtgcccag gcggccaggt cagcgcggtc gtctgcgccc
      961 gtcagggtcg caggctgcga cagcggcagg ccgcgctcca tcgcgagagt cttgacgggg
     1021 ctccccgcaa gctgccggcc gcgccctcgc ggcctgtcag gcctggtcag gactcccacg
     1081 acgtggtggt gcgagtccag cagagcgagc aaagcgcgta gcgcgaagtc gggggttccg
     1141 gcgaaagcga tgcgaagaga gctcacaccg cgcggaatca atacatgcgc ttgggacgcg
     1201 tggatccttc cgagctcttc tgtgcccgct ccttgcgctc cttctccagt ttcttacgga
     1261 tccgctggcg cttcagatcg gacaggtagt ccacgaacag cttgccctcg agatgatcca
     1321 tctcgtgctg gatgcagacc gccagcacct cgtcgcagtc gcgctcgaaa gtctggcccg
     1381 tgacatcctg cgcgcgcacg cgcaccttcg ccgcccgctc cacatcctcg aagatcccgg
     1441 ggaccgacag acagccttcc tcgctttctg ccttaccctc gcgcgtcagg atctcgggat
     1501 tgatgaagac cagcggctcg gtgcgctctt tcgagatgtc gatgacgatc acgcgctgat
     1561 gcacatcgac ctgggtcgcc gcgaggccga tcccgggagc ggcgtacatg ctctcgaaca
     1621 tgtcctcgac gagctgcttc agcccggcat cgaaatgggc cacgggctgg gcgcgcgtgc
     1681 gcagccgcgg gtcgggaaac tccagaatgg ttcgggtcgc catgggcttc aaactataag
     1741 ggagaacatg tatttgattc aaaaatactg ctaatgttca atagcttgcg ggagttttga
     1801 ggcgctccgg gtcggcggcc ctatacagcg gccgcggccg tgacccagtg cacaaagttg
     1861 acattaaatc atgtgctggc gctgtcgctg ccagtataac aaccgggatg ctgcggcggg
     1921 gtcgtacgcc ctcgccagga ccccagattg ccggggcggc gtgcgccacc tgcgggaaac
     1981 ccaagggcgc agcccattga ggagtggaga tggtctcaaa tcgcatacct cgggcttgcc
     2041 tgtgcgcgag cctgctcatg gtgctgttcg ccgtcggtgg ttgctcctgg ttgcaccggg
     2101 gaagtgggag cccggcagtg caggcagcgc ccccgagcgc gcctccgccg gattccgccg
     2161 aggcgcctcc gatggacgag ctcaccgcca ccgaggccgc cgcaagcggc gcgggcgcct
     2221 cagcgaccgc agcgtccgcg cccagcagcg ccgatgccag cagcatcgtc aatccgagcg
     2281 cgcccaagag ctacaccgtc aagcgtggcg acaccctctg gggtatcgcc tcgatgttcc
     2341 tcaaggatcc gtggttgtgg cctgaggtgt ggattatcaa ccccaaggtc gccaatccgc
     2401 atttgatcta ccccggggac gtcctggcgc tcgcgtacgg ggccgatggc cgcccgcaga
     2461 tccgcctgga tcaggctggc gccgcacgcc tggagccgcg cctgcgcagc tcgccgctcg
     2521 atgctgcgat tccgacgatc ccgtattcgg cgatcgccgc gttcctctcg cgaccgacgg
     2581 tcctgtcgtc ggagcaggtg cgcgatgcgg cacacatcgt ggccttccgc gacgagcatg
     2641 tcgtcgccgg cactgggcac gatgtctaca tcagcggttt gaatgcgcag cccaacggcc
     2701 gttacagcgt cgtgcacatc gccgacgagc tgcgtgatcc ggacgataac aaggttgtgg
     2761 gctacgaggg tgtctatacg gcgaccgctc tggtcgccaa tccgggtcgg ccgacgaagg
     2821 ctctgttgat cgatgccgcg cgcgagactc tccagggaga caaggtgctg gcggccgacc
     2881 tcgatgtgcc gctgaatttc atgttgcgtg cgcctcgtaa tgaggtgaac gggcggatca
     2941 tctccgtcat cgacggcaca caggtcattg gccagtacca gatcgtggtc atcaaccgcg
     3001 gcaagcgcca tggcatcgat gccggtcacg tgctggcgat cgatcaggcc ggcgaggtgg
     3061 tgcacgacgt gtacggcggc gccaccggcc tcagccgcta tctcggccag gcaggctcct
     3121 cgctgtctcc gaaggtcagg ttgcctgatg agcgcgccgg cacgctgctg gtgttcaagt
     3181 cgttcgaccg ggtgagctac gccctgatca tcggtgcgtc caactcgatt cacgtgggcg
     3241 atatcgtcca caatccgtag gatccagcgc ccgccatacc acgctgcaga gcacgcccgc
     3301 cagcggccct ggtcgcatgg cccgtcgtgc ttgcgcatgg gggcgccgcg caaatctgcc
     3361 ttttcaggct gcttttaccg gactgtctcc aggcacacgc acgcttaggc ttgcgcgcat
     3421 ggatgcagtg actgcgcgtg tcgtgttggc ccgagcgccc ggcctcacgg cggcccacgt
     3481 gcatgcgctg atggccgctg ttgattctga catcgagcgc gtcctcgacg ccggcagtct
     3541 cgcccggatc gagctacctg cgcacacccg ggcttttctc gaacaacccg atcgcagcgc
     3601 cgtgctgcag gattgccact ggctggcggc gagcggcgcg cagctgctgc tgagtaccga
     3661 tgcgctctat ccgctgcagc tcgcgggagc acccgacccg ccggcggctc ttttcgttct
     3721 cggtgatccg cgaacgctgc gcgagccgca gcttgcaatg gtcggctcgc gcaatccaac
     3781 tccctgcggt cgccggaccg cgcgcgaatt tgcggccagc ttcgcccgcg ccggattgac
     3841 gatcaccagt gggctcgcca tcggcatcga tgcggcatgt cacgagggcg ccctgcaggg
     3901 tggtggcaag accatcgcgg tttgtgccac gggactcgag cgcgtctatc ccgcccaaca
     3961 tggtgggctt gccgagcgca tccgcgagcg cggcgcgctg gtgtcggagt tcgcaccgag
     4021 caccgcactg cggcgcgcaa actttcccag acgcaatcga ctcatcagcg ggctcgccgt
     4081 gggaacgctg gtcgtcgagg cggcacagcg cagcggctca ttgatcactg cgcgccacgc
     4141 cgcggatcag ggtcgcgagg tgtttgccat ccccggctcg atctacagtc cgctctcgcg
     4201 cggctgtcat aagctgatcc gcgacggcgc caccctggtg caggagccgg cggagctcct
     4261 tgccgagctc agaattcccc ttaaggaaga agcacttgta gagggctcga gaccctctgg
     4321 cgcaggctcg gcattggaca aggggtttga aatgctgtta gatgcggtcg atttcgaacc
     4381 cgcgaccgtc gatgtgctcg cggcccgcac aggacttgcg ggcgaagtga ttacaccgat
     4441 gttgctgatg ctcgagctcg aggggcggat cgcgtcctgt gcgggcggcc gttatggccg
     4501 gctaccttga tcgtgccgca ggcgattttt ctgcggaaat tatgatgacc ggcactgttc
     4561 tagacattct gatctacgta ttcgaccgct acatgctcga tgaggtgccg gaggtacccg
     4621 agcgcgcgca tctggcgcgc gatctggagc gcgtgggatt cacgcatgcc aacgtcgagc
     4681 gcgcgctcga ctggcttgcc gagctggcgt gcgagcgtga ccggcccttg ttgccgcttt
     4741 ccggagaaaa cgacggacct gcggtacgga ttttctccga cggcgagctt gctcgtctgt
     4801 ccactgaatg ccgcggcttt ctcgtcacgc tggagagact tgggatcctc tcaccccagc
     4861 agcgcgagat cgtgatcgat cggatgctgg ccctggatgc cgatgagctc gacacagagc
     4921 agttgaaatg ggtcgtgctg atggtgctct ccagccagcc cggtcaggaa caggcgttcg
     4981 cgcgcctgga ggacctggtc tccgacgttc atcccggcac gccgcattga gcgcggcgcg
     5041 cacggccgcc cggccgatac agtgctgaaa ggggctttca gcagcagccc atgccagaga
     5101 atctcctcat agtcgagtcg cccgccaagg cgaagacgct caagaagtac ctggggaagg
     5161 acttcgaagt gctggcgtcc tacggccacg tgcgcgatct gattcccaag gaaggcgcgg
     5221 tcgatcccga tcgcggcttc agcatgcaat accaggtgct ggagaagaac gagcgccacg
     5281 tcgaggccat ctcgcgcagc ctgcgcaagt cgaaggcgct ttacctggcg actgaccccg
     5341 accgcgaggg cgaggcgatc gcctggcacc tgaaggagat cctgcagtct cgcggcgagc
     5401 tcgatggcaa gcgcgtgcac cgggtcgcct tctacgagat cacgcgcaat gcgatccgtg
     5461 cagccgtcgg tgagcctcgc gatctctcgc tcgacttggt gaatgcgcag caggcgcggc
     5521 gcgcgctcga ctacctcgtc ggtttcaacc tctcgccgct gctgtggaag aaggtgcggc
     5581 gcggactttc agccggccgc gtgcagagtc ccgcactgcg catgatctgc gagcgggaag
     5641 cgcagatcgc cgcgttcatc gcccaggagt actggacgat cgagggcgaa ggctcgcaca
     5701 cgggtcaggc ttttccgctg aagctgctgg agtacagcgg gcgcaaggtc gagcagttca
     5761 gcttcgtcaa cgaggctcag gcgcgcgagg tcgagcgcac gatcgaggcg gccgcgcgct
     5821 ccaccgcgca ggcggcggca cccggcctcg gggagctgcg catcatcgcg atcgaccgga
     5881 aacagcggcg ccgcaatccg gcgccgccct tcacgacctc gacgctgcaa caggaggccg
     5941 cgcgcaagct cggattcaac gctcggcgca cgatgcggct cgcgcagcag ctgtacgagg
     6001 gcatcgatat aggcgacggc agcggcagcg tcggactcat ttcttatatg cgtaccgatt
     6061 cggtgaccct ggctgccgag gccatcaccg aaatcagaga ggtcgcagcg cgggaatacg
     6121 gcaaggccga ggtggccgag gagccgcgca tctacaagac caagtcgaag aatgcacagg
     6181 aggcacacga ggccattcgc cccacttcgg cttcgatcct tccggggccg ctcgaaggca
     6241 agatcgaccc cgatctgtac aagctctact cgctgatctg gaagcgcgcg gtggcctcgc
     6301 agatgagtca cgcgatattc gacaccgtcg cggtggacat gctggccgga aaggatgggc
     6361 cggagcgcca tctgctgcgc gccaacggct cgacgctggt caagccaggg tacatcgccg
     6421 tttaccagga aggcaccgat gactcccgcg tggacgacac cgatcacgcg ttgccgccga
     6481 tggaggttgg cgacatcgtt accctggccg ccttgcgcgc cgagcagcat ttcaccgagc
     6541 caccgccgcg ctactcggag gcctcgctgg tgaaggctct ggaggagcat ggaatcggcc
     6601 gcccctcgac gtacgcgacg atcatctcga ctctgcagga tcgcgagtat gtcgagatgg
     6661 acgcgcggcg attcgtgccg acggatatcg gcaagatcgt caatcgcttt ctgacggatc
     6721 acttccatca ttacgtcgag tacggcttca cggcggccat ggaagatgag ctggatgcgg
     6781 tctcgcgcgg tgaagaggac tggaccaccc cgctcgggaa attctggaag ccgttcatca
     6841 ttcaggtcca gaagatagaa aagaacgtca cccgcgagca ggtcgcccag gctcgcgagc
     6901 tgggtaagga tccggcaagc ggcaagccga tgaccgtgcg aatgggccgc tacggcccgt
     6961 tcgtgcagat cggcaccaag gatgatgagg agaagccgcg cttcgcaggg ctcaggcccg
     7021 ggcagaagat ggatacgctg acgctcgccg atgccctcga gctgttcaag ctgccgcgca
     7081 cccttggagc cactgcggac ggtgagccca tcatcaccaa cgtgggccga ttcgggcctt
     7141 acgtgaagta cggcagcaag tatgtttcgc tgaaggagga cgacccctac accgtcagcc
     7201 tcgagcgggc actcgaagtc atccgtttga aacaggaggc agatgccaac cggatcatcg
     7261 cggacttcag tgccgagggg atccaggtgt tgaatgggcg ttatggacct tatgtcacgg
     7321 acgggaccaa gaacgccaag attccgaaag atcgcgaccc caaatcgctc actccggcgg
     7381 actgccgcga gctgttagcc caggccccgg cccggggagg ccgctttggt cgcggcaggc
     7441 gtggcaccca gtcgcggccg cgtgcgggca atggccaggg cacggggaat ggcagcgagt
     7501 cgcagccgag cccgacgccc agcgacagcc ggcccaagcg cccgcgcgcc tccactgcgc
     7561 aatcggcagc gacaggcacc gacaagcagc agaaggcttc gaatcgagac cgatcccgga
     7621 ccacgcccga agctgcagat cgcaaatccg gcacgggcaa gggggcggcg ggctcgctag
     7681 cgggcgcgag gagcgcggtc acagcgaagt tgggggcggc ggcgaaggct ggcgcgaagc
     7741 cgggccccgc cgcgaagaac acggccgcgc acgcacgtgc accgggttcc aacccgcgtg
     7801 tggggcgagg ctccccagcc aataagcctg cccgacaaag cccggcaggc aagccgcccg
     7861 cggctcgcaa aaacggcaag tccgcagcga accggcctcg cggactgaaa tagccggtcg
     7921 cctgcgcggt gctgtcgcgc acatgactaa gtaccggcac agccatcatg caggcaactt
     7981 cgcggacgtg cacaagcacg tcacgttgct cgcgctgctc gcctcgatga agcgcaagga
     8041 caaggggttc ggctacttcg agacgcatgc cggtcgcggc gcctacgaca aatcagccgc
     8101 ctcttccgag ctcgctgccg gcgccggccg gttcatgacc agccagcaca ccgcaccgga
     8161 gctccgcgag ttcgcctctc tcctgacgca gttccgtctg tcgcgcccgc agcacgcgta
     8221 tcccggctca ccggtgatcg cctcccgcga gctgcgcgcg caggatcgtg cggtcctttg
     8281 cgagctcgtt ccctcggaag cacgcgcgct ggagcgggaa ctcgcaggca atgcgcgcct
     8341 gcgcatcgag cagggcgatg gcttcgacag gctgcgagcg tggatgccgc cggcggagcg
     8401 gcgtgggctc gtgctcctcg atcctgcgta cgaggattcc caggacttcg acagagtgag
     8461 tgcctcgctg gtggatgccc tgcgacgctt cggcaccgga gtcttcatgg tgtggtaccc
     8521 gatcaaggac gagcgcgaga tccgcacctg gcagtctcga atggcgccga tgctcggccg
     8581 cgaggcgctg ctcagcgagc tgtggctgta cccccgcgac tcgaaagtcg cgctcaacgg
     8641 ctccgggatt ctgatcgtga acccaccctg gctgctcgcc gagcgcatgc accgctggct
     8701 acctgagctg caagcgcttc tggataccgg gcacgagggc ggtacgagcg tcacgaagct
     8761 gacatccacg gcgtgaccgg ccttagccgc gggagcagaa tcttcgcatg gagacgcaaa
     8821 aggaccgcta cggcgtcgtc ggccatccgg tgggccacag ctggtcaccg ttcattcatg
     8881 gcctgttcgc gcgggacacc gcgcaggcca tgagctatcg gctgtacgac tttcgaccga
     8941 aggagttcga gccgcgagtg cgtgacttct tcaccctcgg gggccgcggc ctgaacgtga
     9001 ccctgccgca caagggagcg gccgtcaaaa tcgccagcga gctcacttca cgggctactg
     9061 atgcgcaggc ggtcaacacg ctcgcggctc agaaggacgg ctcgattctt ggcgataaca
     9121 ccgacggtct gggcctggtg cgcgatctgt gtgacaacct ggggctggtc gtcactcgcc
     9181 gcaggctgct cattctcggc gctggcggcg ccacgcgcgg cgtgctcggg cccctgctga
     9241 cactcgagcc ttcagagatc gtcatcgcca accggacgac ggagcgcgcc agctcactgg
     9301 ctgaagcttt tgccacgctg ggaccggtgc gcggagccgg tctcgcggag attccggggg
     9361 gtccgtacga tctgatcatc aatgcaactt ccgccggtct gagcggagag attccggcgg
     9421 tcggcgcggg catcatcggc ccggagacga cctgctacga catgtcgtac gggaagagcg
     9481 acacaccctt cacgaaatgg gccctggacc tcgggtgtgc ccgggccttt caggggtggg
     9541 gaatgctcgt cgagcaggcg gccgaatcct tccgggtgtg gcgtgggatg cggcccagca
     9601 ctgcctccgt cctcagcgcg ctcaaagaac gcgcgctcag cgcctcacag gccacagccg
     9661 gggcactcag cgcatcgtga cgagctcttc cgcgctggtc ggatgaatgg ccaccgtatc
     9721 gtcgaagtcg cgcttggttg cccccatgcg cacggccacc gcgaatccct gcatcatctc
     9781 gtcggcaccc ggcccgatga catggacacc caccacacgc tgctcggccc cgaccgtcac
     9841 aaccttcatg tcagtacgcg gcttgcggct ggtcagcgcg tgatacatcg gaatgaatcc
     9901 cgatcggaac acggtcacgg cctcatcccc gtactgcgcg cgagcggcgt cctctgtcag
     9961 gccgaccgtt ccgaccgggg actggccgaa gaccacggtc gggatatagt cgtagtcgag
    10021 gtaacgctcg ggctgccccc cgaaaagccg atcggctagc cgccggccgg cggcgatggc
    10081 cacgggggtg agctgttttc gcccggtcac atcgcccact gcatacacgc ccggactggt
    10141 cgtgacctga aacttgtcgg tcacgatgaa accgtaatca tcgagagcga cttccgcgcg
    10201 ctcgagaccc agctcttcca cttccgggac acgtccgatc gcccagatga cagtgtcaaa
    10261 aggtgtcagt cgccggccgt cccgcacttc gagatccagc gctccggtca cggtccgttc
    10321 gagtgactgg ggccatgcgt tggtcacgac ttccatgccc gcctcacgca tgatcgtgag
    10381 agtcgcctcg ccgagcatgc tgtcgaactc cttgagcgcg gcctgaccac gcagaaccag
    10441 tgtcgcccgg gcgcccagcg cggcgagaat tccggtcagc tcgatggcga tatagccgct
    10501 gccgaccacg gcgactcgct cggggagctg cggcagctcg aagaagccat cggaggtgat
    10561 tcccagctcc atccccggga tggcgggcag cagcgggcgc cccccggtcg cgatgagaac
    10621 gtgggtggcg gcgagcttgc gcccggaggc ttcgacggtg tgtgcatcga ggaaggttcc
    10681 ccgacctcgc aggagctcga ctccgcgctt cgccagattg ctttcgtaga tgccgttgag
    10741 gcgtgccacg taggcgtccc gcttgctctt cagcagcgcc cagtcatgcg gctcggcggc
    10801 acccaaccgg aagccgtact cgctcgcatc ggcgaggcaa tcggcgatca tggcggcgtt
    10861 ccacatgatc ttcttgggca cacagccgac attgacgcag gttccgccga gccggccgga
    10921 ttccaccagc gcgacccggg cgccgtactc gctcgctcgc tgggcagccg cgaggccgcc
    10981 gctccccccg ccaatcacca cgagatcgaa ctggtccgtc atagcgcacc tgcgaccgaa
    11041 gctgccgcat atcatgttac atggggctcg gggacgggcc cgccatctct cattcggact
    11101 gcgcgggacg cccacaaagt cctagaatgc agctccttca tggacgggag ccgcatggaa
    11161 aaccccctgc tgggcctcga gcccctgccc gcatttgccc ggatccgccc ggagcacgtc
    11221 gagccgggcg tgcgagcgct gctggagagc agccgcgcgc ggctcgagga gctggtggcc
    11281 gtggagaatc cgacattcgc cacggtcatc gagcctctgg aggagctgca tcatcgcgtg
    11341 acccggacgt ggtcgccggt gagtcacctc aacgccgtgc tcaattccga ggccttgcgc
    11401 accggctaca acgcgtgcct gccgctgttg tcggcctatc agaccgatct ggcccaaagc
    11461 gagccgcttt accgcgctta ccgcgccatt gcagaacagg aaggcgccgc ccttggaccg
    11521 gtgcagcgtc agctcatcga gcacgccttg cgcgacttcc gcctcgccgg cgtcgggctc
    11581 gacactgcac gcaagcaacg cttcaaggcg gtgatgctcg agctgacgca actgcaggca
    11641 aagttcgagg agaacgtgct cgatgcgacc aacggctgga gctgccatct caccgaagag
    11701 tccgagctga caggcctcaa ctcgacgctc atcgagcagg cgcgccgtcg cgcacacgag
    11761 cgcggaatgg agggttggat actgattctc gatcagccca cctacgtcgc cgtggtcacc
    11821 gacgcggagt ccgaagcctt gcgacgctcg ttttacgaag cctggacgac ccgcgcatcc
    11881 gaccagggtc ccaatgccgg acgctgggac aattcacagg tgatggaggc cattctgcgg
    11941 ctgcgccacg aggccgccca gcttctgcaa ttccgcaatt acgcggaata tgcgttgtcc
    12001 acgcgcatgg ctcgcagcgt cgaggaggtc atgcagttcc tggtcgagct cgcccgctcg
    12061 gcgcgcgatg ccgctcatcg tgagtttgcg gagctggagg cctttgcggg ccgcaagctc
    12121 gccgcctggg acgttggttt ccacgcggag cggctgcagc gcagccgcta ttccgtatcg
    12181 caggaggagc tgcgacccta cttcccgttg ccgcgcgtgc tggccggatt gttcgaggtg
    12241 ggacagcggc tcttcggcct gcgcatccac gagcgtcacg gcgctccggt ctggcacccg
    12301 gacgtccgct tcttcgagat cgagagcgcg gccgggcagc tggtcggcag cttctatctc
    12361 gatgcctatg ccaggcccag caagcgcagc ggcgcctgga tggatgagtg cgtcggtcgc
    12421 aagcgcctcc ccagcggctc ggccctgccg gtcgcctatc tcgtgtgcaa ttttctgccg
    12481 ccgagtgagg atcatccggc cctcctgact cacgacgacg tgctcacctt gttccacgag
    12541 ttcggccacg gactgcacca tctcctgacc cgcgtggact atccgagcat cgcgggcatc
    12601 aacggtgtgt cgtgggatgc agtggagctg ccgagtcagt tcctggaaaa ctacgcctgg
    12661 cacccggaag tgctgcagcg gatctcgagc cacatcgaga caggagcgcc gttgccggcc
    12721 gaaacgcagt cgcggctgat agcgacccgc agcttccacg ccgggctgca gatgatgcgg
    12781 cagctcgagt tcgcgctgtt cgacttccgt ctgcacgcgg agtactcgcc cgagcggggt
    12841 gcgcgcgtgc tcgaggtgct cgagcaggtc cgccgcgaag tcgccgtggt cccggcaccg
    12901 ccctggaacc gcttccccaa cagcttcggg cacatctttg ccggcggtta tgccgccggg
    12961 tactacagct ataagtgggc ggaggttctg gcggctgacg ccttcgcagc attcgaagag
    13021 agtggcgtat tcgacatgct gaccgcccat cgcttcctcg atgcaatcct cacacgcgga
    13081 ggaagccggg acgcgctcga ggcattcatc gaattcaggg ggcgaaagcc cgatgtgcgg
    13141 gcactgctca agcagcacgg gatactgacg gagagcccgc ccgccgcgtg agtcgatgtg
    13201 acctttcctg gccgagcttg cggcttgaca tacaacgcgc gccagctggg gtcgaggatc
    13261 aagagcttac ggagtctgcg gctgcacagc cgagcagccc tgccgccggc cctcaaaagt
    13321 gtgcgttgtg tcgctaacac tcccctcggc cgctggccta gacttccggg cggtcgggtc
    13381 gaaactgggg acggccattt agccgcgcgg acatcaagag aatgaaaagc gttcggcgca
    13441 gcgatttcga tgtaaccaac acggtgcagg tgagctcgcc tccggcggtt ctggccgagg
    13501 tcagaagtct gttcgagccc acgtggccga cactctcctt cgagcccctg gatcgcgcct
    13561 tccggcactt cgagcgactg ttcgccggag acgtgcccgg ctactgcggt gtggacacgg
    13621 tctatcatga tcgccagcac acactcgata tcacgcttgc gctggcacgg ctgctcgttg
    13681 gctacgaacg gcaacacggg gaagcggagc gcctgggcgg cagccgggct ctgatcgggc
    13741 tgatcaccgg cctcttccac gacgtcggct acctgcggcg gctggatgac ctctcgatcc
    13801 gcaacggcgc ggagttcacg cgtacgcacg tctcgcgcgg cgcccgtttc ctgcaggact
    13861 atctgcccga gctgggactc gccgacgccg taccgctggc gtgcgaaatc attcacttca
    13921 ccggctatga ggtgcccttc agccagatca aggtcaccga tccgcgcgac atcgccgtgg
    13981 gccatctgct gggcacggcg gacatgatcg cgcagatggc cgatcgatgt tatctggaga
    14041 agtgccgcga ccggctgtat gccgaattcg ttctgggtgg cgtcgcgctg ccaatcgccg
    14101 caaacggggg tcgcgaggtg aagtacgcct ccggtctcga tctgctgcgc cagacaccgg
    14161 agttcattgc cgaggtccgt gcgaaacgtc tcgatggcga gttccattct gcctatcgca
    14221 gtctcgagat cctctacggc ggtcgcaacc cctacatcga ggcaatcgac cgcaacgtgg
    14281 actacttgcg gcagatcctg cgcagtgaga actggcgcct gctccggcgc aacccgccga
    14341 tctttgccgc gaccgatgac ccgatgtcca ccatgcgggg gctgatgctc ggctacatca
    14401 agcgggtctg ggcgagcaag tacgaagaaa gctgatctgc gcgaatccgg ctgtcagacc
    14461 gcggcgcccc gctgtcacga taccggcgcc tcgaccgcgc acacgtaccg ttcgtaaatc
    14521 tccggcaacc gctcgaggac ctgcgcctga cccggcatgt ccgttgcagc aatggtctgc
    14581 gccaagatgt ccagcgagag cgcgtccagc gccggcaccg ctaccggacc gtagctcaga
    14641 tgccacggct ccggttgcac gccgccgcga tcgatcgtgt aggggcggaa gaatccgaag
    14701 cggcccatgt tctcagtcag ccagcgatcc aggtgcgcga agcaaccgcc gggtgcgaac
    14761 tcgtgcggaa ccagttgcgg gcgcccgcca gcggggagcg atgccaggtc gatgacatcg
    14821 atgtcggtgc cccagtgatg acggctggcg ccaggtagtg ccgaccagat caggatggcc
    14881 tcgatgagcg cgggctcatc caggctggca tgcgcgatct cgtttccctg ccgatcgagc
    14941 aggcgccgct cgccccggaa cttcgcgctc cagatgccga gctggcgctc gaaatcccgg
    15001 aacgccgagg cgatgccaac atcgatccct gcctcgcgag ccgcctcgcg catccgcagc
    15061 agcgcccggc cggcatccgg ctgcagccga cagctcagct cggcgagctc ctgcacgtgc
    15121 gtcgcagcgc gtccggtcaa ctcgagggcg gtgagcattc g
//
LOCUS       DASRHN010000082         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1675653, whole genome shotgun sequence.
ACCESSION   DASRHN010000082 DASRHN010000000
VERSION     DASRHN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675653"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFB37_03125"
     CDS             complement(<1..636)
                     /locus_tag="VFB37_03125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO21470.1"
                     /translation="MITKFDSLFAGHTDLENVGYGGTPINERRYPNEHLATALSKAEN
                     MAKLMDGLGYNCFWTAEHHFQREGTECIPNNILMAVHLAHCTKNIKIGCGFNITPMWH
                     PLRLAEDYAMADILTGGRVVFGVGRGYHTREVETFGSPLRDQEANRELFEEQVDIIFK
                     AFNSEAFSHKGKHYTLPPEIPYRGYTLKELTLVPRPLRLPVECWQPIQGGST"
     gene            complement(667..1185)
                     /locus_tag="VFB37_03130"
     CDS             complement(667..1185)
                     /locus_tag="VFB37_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21471.1"
                     /translation="MLYMVEMDFRNPAREADWHVWYLGHVAKLIRNVPGFRASQRFRA
                     ITPTPSPWLALHDVASAAVFESAEYRANGGPASTGEWQAEHLNWHRNLFSGIDNTPEV
                     PPDAHLLLAEAGAEVPEPHAATLTRLEPVGLDRTSKGVALGVIPAGRLTAGLFGLPGV
                     RLFKPISPRITK"
     gene            complement(1308..1712)
                     /locus_tag="VFB37_03135"
     CDS             complement(1308..1712)
                     /locus_tag="VFB37_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZO21472.1"
                     /translation="MADSELYRKGEAIRRRLRGEADFARNQADYARDPVMKKFIDVAT
                     ETVFGALWTRPGLDIKTRTLVCVVSDAATGRHPELDIHLRFALKEGWTEEQLTETLLH
                     LTGYVGVPIIREAMLVASKVFAEVRAERAGKA"
     gene            complement(2049..2798)
                     /locus_tag="VFB37_03140"
     CDS             complement(2049..2798)
                     /locus_tag="VFB37_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HZO21473.1"
                     /translation="MRQPRSGNTWVQPVTSLSEPNRSNPFEHLTFMTFRGDSQRTTLL
                     YLVAGLMLACWCSCIASAQTSGRFSGDLVLKVLPDGRTMELVQPFAYTDSRGVAWPVP
                     ARTRVDGASIPSAFWSIIGAPFTGKYREASVIHDYYCASRSRHWKAVHKVFLDGMLAR
                     GVDKIQAQLMYLAVYRFGPRWDFDVDACFCQGCPTCANPVVKRIPSYQPTYKAADFQE
                     LRSKLMAGGSTLEELEDDADYQLNTEILKAR"
     gene            complement(2747..3475)
                     /locus_tag="VFB37_03145"
     CDS             complement(2747..3475)
                     /locus_tag="VFB37_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21474.1"
                     /translation="MRRPFVLQTLLLSSMLFGDCAVAQRTYTFEEACRNAGWTTGPCA
                     PQQQAQPLGCVPLGGNAFHAEAFSGNCIPATLSPALDAEIFIEMIGLPNIPKINELRL
                     TIGRTSNQNNAFATFHNGGRLLIIDPVWARSGVEAYLIIGHEAGHHFCGHTLPGFKGR
                     PKEAELEADRFSGASIKRFEIYHRRAFFTEALKAAERLYSEDGNRTHPPRAARINAIT
                     QGYLSGSPCGNLAPAIPGFSPSPR"
BASE COUNT          653 a         1149 c         1303 g          671 t
ORIGIN      
        1 cgtcgagccg ccctggatcg gctgccagca ctccaccggc agcctgaggg gccgcggcac
       61 gagcgtcagc tccttcaggg tgtagccgcg gtaggggatc tcgggcggca gcgtgtagtg
      121 cttgcccttg tggctgaagg cttcgctgtt gaaggccttg aagatgatgt cgacctgctc
      181 ctcgaacagc tcgcggttgg cctcctggtc gcgcagcggc gagccgaacg tctccacctc
      241 gcgcgtgtga tagccgcggc ccaccccgaa cacgacgcgg ccgccggtga ggatgtcggc
      301 catggcgtag tcctcggcca gccgcagcgg gtgccacatc ggcgtaatgt tgaatccgca
      361 gccgatcttg atgttcttcg tgcagtgggc gaggtgaacg gccatcagga tgttgttggg
      421 gatgcactcg gtgccctcgc gctggaagtg gtgctcggcc gtccagaagc agttgtagcc
      481 caggccgtcc atcagcttgg ccatgttctc ggccttgctc agagcggtgg cgaggtgctc
      541 gttggggtag cggcgctcgt tgatgggcgt gccgccgtag ccgacgttct cgagatcggt
      601 gtggccggca aagaggctgt cgaacttggt gatcatgggg cgtctccctg ctgggcaggt
      661 gtgaggctac ttggtgatgc ggggcgagat gggcttgaag aggcgcacgc cgggaaggcc
      721 gaacaggccg gcggtcagac gaccggccgg gatgacgccg agcgcgacgc ccttggaggt
      781 gcggtcgagg cccaccggct cgaggcgggt gagcgtggcg gcatgaggtt ccggcacctc
      841 ggcgccggcc tcggcgagga gcaggtgcgc atcgggcggc acctcgggcg tattgtcgat
      901 cccggagaac aggttgcgat gccagttgag gtgctcggcc tgccattcgc cggtggaggc
      961 cggtccgccg ttggcgcggt attcggcact ctcgaacacc gctgccgagg cgacgtcgtg
     1021 cagcgcgagc cagggcgacg gggtgggggt gatggcgcgg aagcgctggc tcgcccggaa
     1081 gcccgggaca ttgcggatga gcttggccac gtggcccagg taccagacgt gccagtccgc
     1141 ctcgcgggcc ggattgcgga aatccatctc caccatgtag agcatggggc gcgcctcctc
     1201 acgtcgttgt cgtggcggcc acattacgcg ggccgcagcc gcgcgcacag agccgccggc
     1261 gcagggcagc caaacggcaa tgggagctgg ctgcggcgac agctgcgtca ggccttgccg
     1321 gcgcgctcgg ccctgacctc ggcgaacacc ttggaggcca ccagcatggc ctcgcggatg
     1381 atgggcacgc cgacatagcc ggtgaggtgc agcagcgttt ccgtcagctg ctcctcggtc
     1441 cagccctcct tgagggcgaa gcgcaggtgg atgtcgagct cggggtggcg cccggtcgcc
     1501 gcgtccgaga cgacgcagac gagcgtgcgc gtcttgatgt cgagaccggg gcgcgtccac
     1561 agcgcgccga acacggtctc ggtggcgacg tcgatgaact tcttcatgac ggggtcgcgc
     1621 gcatagtcgg cctggttgcg cgcgaagtcg gcttcccccc tcagacgcct gcggatcgcc
     1681 tcgcccttcc ggtagagctc gctgtcggcc attggctcgt ctcctcacct attgatcgag
     1741 cgaggctaac cgcggtccga cgggttgtca cgggaccagg caggacgatg ggctcgccgc
     1801 gcccgagctc acctgcgacg gtggaacgtg agacggacgg tccccggccg cgaaaggagg
     1861 caatcgcacg gccacaagag gcagcggaag ccgccgtcgc acgatcaggg ccctgctcgt
     1921 gcaggctggc gcgacatccc ttgctgcctc cgcgcatccg cacgcctttc ccccgcgctc
     1981 gagatgtcgc gccgctgctg cgatgagccg caagaggcag ccgaggatcg ggggctgccg
     2041 ccgacgcgct atcgcgcctt gaggatctcc gtgttgagct gatagtcggc gtcatcctcg
     2101 agctcctcca aggtggagcc tccggccatc agcttgctgc gcagctcctg aaaatccgcg
     2161 gccttgtacg tcggctggta gcttggaatg cgctttacaa ccggattggc gcaggtcggg
     2221 cagccttggc agaagcacgc gtcgacatcg aaatcccatc ttggtccgaa ccgatagacg
     2281 gcgagataca tcaattgagc ctgaatcttg tcgacgccgc gtgcaagcat tccgtcgagg
     2341 aaaaccttgt gaaccgcctt ccaatggcgt gagcgggaag cgcaatagta gtcatggatg
     2401 actgacgcct cgcggtattt tcccgtgaat ggagctccaa tgatgctcca gaacgcgctc
     2461 ggaatggatg cgccgtcgac gcgagtgcgg gccggaacag gccatgccac gccacggctg
     2521 tccgtatagg cgaagggctg caccagctcc attgtgcgcc cgtcaggcag gaccttcaga
     2581 acgaggtcgc cgctgaaccg gcccgaggtc tgtgcgctgg caatgcagct gcaccagcac
     2641 gccaacatca agcctgcaac caggtacagc agcgtcgtgc gctgggagtc tccgcggaag
     2701 gtcatgaacg tcagatgctc gaaggggttt gaccgattgg gttcgctcag cgaggtgacg
     2761 ggctgaaccc aggtattgcc ggagcgaggt tgccgcatgg ggagccggag aggtagcctt
     2821 gggttatggc gttgatgcgg gcggcccgtg gtgggtgcgt acgatttcca tcctcggaat
     2881 ataggcgctc agccgccttc aaggcttccg taaaaaaagc cctgcggtgg tagatttcga
     2941 acctctttat cgaagcgccc gaaaagcggt cagcctccag ctcggcttcc ttcggcctgc
     3001 ctttaaaacc gggaagagta tggccgcaga agtgatgacc ggcttcatgc ccgatgatca
     3061 ggtaggcctc cacacctgat ctggcccaca cgggatctat gatcagcagg cgacctccgt
     3121 tgtgaaaggt ggcaaatgcg ttgttctggt tgctggttcg accgatggtg agtcgcagct
     3181 cgtttatctt cgggatattc ggcaggccaa tcatctcgat aaaaatctcg gcgtccagcg
     3241 caggcgacag agtcgccgga atgcaatttc cagagaaggc ctccgcatgg aaggcgttgc
     3301 cgccgagcgg cacgcatccc aaaggctggg cctgctgctg cggcgcgcat ggccctgtag
     3361 tccacccggc gttccggcag gcctcctcga aggtgtaggt tcgttgagcg acggcacaat
     3421 cgccaaacag catggacgag agcagaagag tttgaagaac gaacggccgt ctcatccact
     3481 gcctccgtcg ggaaaatcaa tcaacaggcc gcattcagga tacctgccga gggaggcaca
     3541 gtccaagcca acactcggcc gacggctgac tttatctcta gccgcaggtt gccgtcgaga
     3601 tcatttggac atggccgcgc gtgtgcagtg ccggaatggc tgggcgtacc ctggctcagg
     3661 gcgcacggcg ggagacgagt gcggtgatcg cccgatggtc gctgacgccc tggtgcaggg
     3721 tcaccgcgct cacgtcgtag ttgggcgtgg agaacatgcc gtccaccatc aggcgg
//
LOCUS       DASRHN010000083         9613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1509788, whole genome shotgun sequence.
ACCESSION   DASRHN010000083 DASRHN010000000
VERSION     DASRHN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509788"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFB37_03150"
     CDS             complement(<1..666)
                     /locus_tag="VFB37_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21475.1"
                     /translation="MGSIVRPGRAAMLLVPLCLSLFTIDSVQATPLEVYGHLPSVEDV
                     ALSPDGSKLAFVRTTEDLRGLAVVGVADHKFLGGAKLGDMKLRSLSWADDDHLLVTVS
                     TTEKAYGTVGTGEYFHLFVYEVSSHKRREYPQYLTETTTQTHTRMMNVLAGSVMVRRM
                     NGETNLFIPGVYVDEGSRSSAFQNIEQWKLLLLRVNLASGKESLVRKGDTSTRDWLVD
                     ESGE"
     gene            750..1475
                     /locus_tag="VFB37_03155"
     CDS             750..1475
                     /locus_tag="VFB37_03155"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HZO21476.1"
                     /translation="MYNSERVLEFPLLLNARDLGGYPTLDGSQTRWRSLLRSDDLAQL
                     TPAGLQALAAYGVETVVDLRWDEEIALAPSPILQQSNIHYVHISLLASSSGRWRELSQ
                     SCEKERWKCLVLEQARPELQEVLRAIATASPGPLLFHCVAGKDRTGLIAALLLTLADV
                     QPDAIADDYAASTRMLGDAYLKRYANTDPSDILENVRCPEQGVHNMIDYLQREGGIRQ
                     YLHKIGLSAQEVDLLRARLRERC"
     gene            1469..2317
                     /locus_tag="VFB37_03160"
     CDS             1469..2317
                     /locus_tag="VFB37_03160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO21477.1"
                     /translation="MLTVLVTGATSGIGLESARVLAGQGHSVLLHGRDAARGEAARRL
                     VNAAGGPHARYLQADFASLAEVRKLARTIQESVPRLDVLINNAGGVNFTRSVTLDGYE
                     RTFAVNHLAPFLLTNLLLPKIRQHTPARIVTVASRAHRNQQIDFADLMSARGYRPRRA
                     YGRSKLANILFTRALAKRLAGTGVTANCLHPGIVATGLGQNNILARALVRLIQSSRGV
                     STAEGARTSLYLATSRDVENVSGEYFSDSRPAPLLTRPEALDDSVAEQLWRSSEELVG
                     LPCTSY"
     gene            2302..3087
                     /locus_tag="VFB37_03165"
     CDS             2302..3087
                     /locus_tag="VFB37_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZO21478.1"
                     /translation="MYFVLKALARTLVLPPAGPLIVASIGLVLLWRQRRIAGGSLLLA
                     GLISSWLLATPVVADALSALAEHYPPLDLSAATGAQAIVILGGGGERLIAPEYAGSVA
                     DSVLLERLTYGAYVARRTGLPVLVTGTPAEAEAMQVTLSRNFGLQVRWIDARSHDTYE
                     NAHYSERLLHAAGVRRIILVTSSTHLWRATHEFQDVGLEVVPAPAGVLARREAGLFRW
                     VPTPVALMRAHAALYELIGEPLRKLQHASGVRERFDKRASTGT"
     gene            complement(3095..4561)
                     /locus_tag="VFB37_03170"
     CDS             complement(3095..4561)
                     /locus_tag="VFB37_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZO21479.1"
                     /translation="MDKIQHVVVLMLENRSFDSVLGKLYPASEAFNGLTGEEFNLDPA
                     GARVSVWNAAGQDPRSMAIPAPDPGELFTDINTQIFETASPAQDAAASMGGFVKSYAA
                     QAADGTPAGTYDPRAVMHFYAPEQVPVLSELATKFAVCDQWHASAPCQTWPNRFFVHT
                     GTANGYENNSPAHFPYRMPSVFTRFDQVGMADAWRIYFHEVPQCLTLTDLWGHLERFR
                     LYAQFRDDAASGALPAYSFIEPRYFPDVELPNDQHPPHVVTLGEQLIADVYNCVRGGP
                     AWPETLLVITYDEHGGCYDHVGPPAAVPPETPQPGQIFKFDRYGIRVPAVIVSPYIRP
                     GTILRPAAGSKPFDHTSLIATLRKRFGLGAPLSSRDGSAPDLSGAFSLPTPDNGGPAR
                     LDAMAYTPTPGEVARARQQPLNGMQTALTRLAAHLPDLTGAANPAAAIDAHIQALSAA
                     PAVAAAGDKVLTPPEALATRSDALHFIKQRMQALFSAF"
     gene            complement(4677..5303)
                     /locus_tag="VFB37_03175"
     CDS             complement(4677..5303)
                     /locus_tag="VFB37_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZO21480.1"
                     /translation="MKRARQKNGEPRFERKLPEARRKALIEATIESLKRFGHEGLSIR
                     RISAQAQVSIGLINHHFPTKGALVAEAYRYFNNELVANLRQAVADAPHAPRARLSAFF
                     LASFSPPNLDSDVLAVWVVFWGLYRHSKDIQRVHSETYHGYVDLLRGMLADLEREVGE
                     LRFNLRLAAIGLTALIDGLWLEWCLDPDNFEPLEAVQLCEAWTERLGS"
     gene            complement(5304..6935)
                     /locus_tag="VFB37_03180"
     CDS             complement(5304..6935)
                     /locus_tag="VFB37_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO21481.1"
                     /translation="MSDSSRPCDALIIGGGHNGLVCAAYLAAGGLRVTVLERRGVVGG
                     AAVTEEFHPGFRNSVAAYTVSLLNPKVIRDLELPRHGLRVVERLVSNFLPTQDGQYLR
                     TGGALTRSDVARFSQKDAAALDAYGERLDAVADVLRDLVLETPPNVVQGDWRSALPEL
                     LRASRVGGRLRKLDMALRRELLSLFATSAGDYLDNWFESDPIKAVYGFDGIVGNYASP
                     YTPGSAYVLLHHVFGEVNGKKGAWGHAIGGMGAITQAMAQSALSRGAQIRVATPVEQV
                     VVEGGRAVGAVTASGEVIRASAVISNLNPKLLYLGLLEPAALPAEFRARISHYRCGSG
                     TFRMNVALSELPDFTCLPGKARGEHHTAGIILAPTLRYMEQAYFDARTFGWSKRPIVE
                     MLIPSTLDDSLSPPGQHVASLFCQHVAAELPGGESWDAHREQVADLMIETVNQHAPNF
                     KASVLGRQIMSPLDLERTFGLVGGDIMHGSLQLDQMFSARPMLGHGNYRGPLPGLYMC
                     GAGTHPGGGVTGAPGHNAAREILADFKRGRLKRAA"
     gene            complement(6973..8163)
                     /locus_tag="VFB37_03185"
     CDS             complement(6973..8163)
                     /locus_tag="VFB37_03185"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZO21482.1"
                     /translation="MDEEACSLPAWIYHDPEFFELEKQMIFRQSWQIVCHQSDVPEAG
                     DYHSLEFLGESVIVLRGEDGEVRAFHNACRHRAAKLLDGPRGHCGRRITCPYHAWTYG
                     LDGRLLTIPQREAFKGLDKARHGLVPLEHEVFLGFIFVRFAPGLPSVREMAAPYAHEL
                     APYRMEELVPHGRVSLRPRMVNWKNVADNFSDGLHINVAHPGLTRLFGRGYGIEAKPW
                     IDKMWGELREIPSSNWSERLYQSVLPPVEHLPAERQRLWTYFKLWPNVAFDIYPDQID
                     FMQFLPVSATETMIREIVYVHPDSRREMKAARYLNWRINRRVSLEDKALIERVQSGMS
                     TSGYTVGPLSEDEVCLRSFGRRMRGLIPETRLLRAPAKGWSDRRTAPELPAFTPQILP
                     QEVA"
     gene            complement(8256..8672)
                     /locus_tag="VFB37_03190"
     CDS             complement(8256..8672)
                     /locus_tag="VFB37_03190"
                     /inference="COORDINATES: protein motif:HMM:NF027545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR/PupR family sigma factor regulator"
                     /protein_id="HZO21483.1"
                     /translation="MSRADRISDEAANWLIRVEVESSPELWQGLHAWLDADPRHRAAF
                     VRLRVAWHRVDALKNLRPLDGTVDADLLTQVRLTPTPTDPVGSPTAARDVRGGRRDRA
                     LGSFYRWAFVACAAVAAAVALAWLLTATPVGLLVSR"
     gene            complement(8675..9238)
                     /locus_tag="VFB37_03195"
     CDS             complement(8675..9238)
                     /locus_tag="VFB37_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO21484.1"
                     /translation="MSKNEERYLLQALELEGSLRARLHRYAGEKCEVEDLLQETYARL
                     LAAAAADAPEVHSIREFAFGIAGDVAADYLRQEHAQPVAIGPELEALAADEPGEPVEE
                     IVKRRQDLARLVSALQRLPERCRQVFTLRKVYGYSPAQIASRLGVPVRLVLEDLRAAA
                     RRCAQMVFDPPALQPTEGWLARLRLHL"
BASE COUNT         1732 a         3074 c         3140 g         1667 t
ORIGIN      
        1 ctcaccgctc tcgtccacga gccaatctcg tgtgctggtg tcgccctttc gcaccaggct
       61 ttcctttccc gaggccaggt tgacacgcag aaggagaagc ttccattgct ctatgttctg
      121 gaaggcggat gaccgggagc cctcgtcgac gtacacgcca gggatgaaca ggttggtctc
      181 gccgttcatc cgtcgcacca tgacagaacc agcgaggacg ttcatcatcc tggtatgcgt
      241 ctgcgtcgtc gtctcggtga ggtactgcgg atactctctg cgcttgtgtg agctgacctc
      301 gtagacgaag agatggaaat attctccggt ccctacagtg ccgtaggcct tctcggtagt
      361 cgagaccgtg acgagcagat gatcgtcgtc ggcccacgag aggctgcgca gcttcatgtc
      421 gcccagcttg gcgccgccca ggaatttgtg gtccgccacc cccacgaccg ccagtccccg
      481 aaggtcctcg gtcgtgcgca cgaaagccag cttcgagccg tccggggaga gcgcgacatc
      541 ttcgacgctc ggcaggtgcc cgtagacctc gagtggcgtc gcctgaaccg agtcaatggt
      601 gaaaaggctg agacacagcg gcacgagcag cattgctgcc cggccgggcc ggacgatgga
      661 ccccatgcgc atgctctccc tttattatct tacgggcgca ccctcgcacc cttgagcatc
      721 atccctcaag agtaagtaga ctttgggtga tgtacaactc agaacgcgtg ctggaattcc
      781 cccttctgct gaacgcccgg gacctggggg gctatccgac cctggatggc tcgcagacgc
      841 gctggcgctc gctgctgcgt tccgatgacc tcgcccagct caccccggca ggtttgcagg
      901 ctctggccgc ctacggagtg gagaccgtgg tcgatttgcg atgggatgag gagatcgccc
      961 tcgcccccag ccccatcctc cagcagtcca acatccatta tgtccacatc tcgctcctgg
     1021 ccagcagctc cggccggtgg cgggagttga gccagagctg cgagaaggaa cgctggaaat
     1081 gtctggttct ggagcaggcc cgtcccgagc tgcaggaagt gttgcgagcc attgcgaccg
     1141 cctcgcccgg gccgctgctg tttcattgtg tcgcaggcaa ggatcgcacg ggcctgatcg
     1201 ccgctttgtt gctgacactg gcggacgtgc aacccgatgc gatcgcggac gactacgcag
     1261 ccagtacccg gatgctgggc gatgcgtatc tgaagcgcta tgccaacacc gacccgagcg
     1321 acattctcga aaacgttcga tgccccgagc agggcgtcca caacatgatc gattatctgc
     1381 agcgtgaggg agggatccgg cagtatctgc acaagatcgg gctcagtgcg caggaggttg
     1441 acctgctgag ggcgaggctg cgcgagaggt gctgaccgtt ctggtgaccg gggcgaccag
     1501 cggaatcggg ctggagagcg ctcgcgtgct ggccgggcaa gggcattcgg ttctcctgca
     1561 cggccgggat gcggcacgcg gagaggctgc gcggaggctc gtgaacgcgg cgggcggtcc
     1621 gcatgcccgc tatctgcagg ccgacttcgc ctcgcttgcc gaggtccgaa agctcgcgcg
     1681 gaccattcag gagagcgttc cgcggctcga tgtgctgatc aacaacgcag gaggggtgaa
     1741 tttcacccgt tcggtgacac tcgacggtta tgaacggacc ttcgccgtga atcatctggc
     1801 tcccttcctg ctcaccaacc tgctgcttcc taaaatccgc cagcacacgc cggcgcggat
     1861 cgtgaccgtc gcctcgcgcg cgcaccgcaa ccagcagatc gatttcgcag acctcatgag
     1921 cgcgcgcggt tacaggccgc gccgcgctta cggccgctcc aagctggcga atatcctctt
     1981 cacccgcgcg ctggccaagc gcctggcggg gacgggtgtg acggccaatt gcctgcatcc
     2041 gggcatcgtg gcgactggtc tcggccagaa caacatactc gcgcgtgcgc tggtccgcct
     2101 catccagtcc tcacggggcg tgtcgaccgc ggagggcgcc cggacctcgc tctatctggc
     2161 gacctcccgg gatgtggaga acgtgagtgg cgaatacttt agcgacagtc gcccggcgcc
     2221 gctgctgacc cgcccggagg ccctggacga ctcggtcgcc gagcagctct ggcggtcgag
     2281 cgaggagctc gtaggattgc cgtgtacttc gtactgaagg cgctcgcgcg aacactcgta
     2341 ctcccgcctg cgggcccctt gatcgtagcg agcatcgggc tcgtgctgct gtggcgtcag
     2401 cgacgaatcg ccggcggctc gctcctgctc gcgggattga tctcctcatg gttgctcgcc
     2461 acgccggtag ttgcggatgc gctgtcggca ctcgccgagc actatccgcc gctcgacttg
     2521 agtgcagcga cgggcgcaca ggcgatagtg atcctcgggg gcggcggcga gcggctcatt
     2581 gctccggaat atgccggctc cgtggccgac tccgtcctcc tcgagcggct gacttatggg
     2641 gcatatgtcg cccgacgaac cggtctgcct gtgctggtga ccggcacccc tgcggaagcc
     2701 gaggccatgc aggtcacgct gtcgcgcaac ttcgggctgc aggttcgctg gatcgatgcc
     2761 cggtcgcacg acacgtacga gaacgcgcat tactcggaac ggctgctgca cgcagccggt
     2821 gtgcgccgga tcattctagt gaccagcagc actcacctct ggcgtgccac acacgagttc
     2881 caggatgtcg ggctggaggt cgtgcctgca cctgcagggg tcctcgcgcg gcgtgaagcg
     2941 ggcctgttcc gctgggtgcc gacgcctgtg gcgttgatgc gggcgcacgc ggcgctctat
     3001 gagttgatcg gcgagccctt gaggaagctg cagcacgcct cgggcgtgcg cgaacgattt
     3061 gacaagcgcg catccacagg aacatgaccc gctgtcagaa ggcgctgaat aacgcctgca
     3121 tccgctgctt gatgaaatgc agagcgtcag agcgggttgc gagcgcctcc ggaggtgtca
     3181 aaaccttgtc ccccgcggct gcaacagctg gcgctgcaga tagagcctgg atatgagcgt
     3241 caatcgccgc ggcagggttg gccgcgccgg ttagatcggg caggtgtgcg gcgagccggg
     3301 tgagtgcggt ctgcatgcca ttgaggggtt gctgtctcgc ccgggcaact tctccgggag
     3361 tcggcgtata ggccatcgca tccaggcggg cgggaccccc gttgtccggt gtaggcagcg
     3421 aaaatgcgcc ggacagatcc ggcgcagagc cgtcccggct gctgagcggg gcgcccaggc
     3481 cgaagcgttt gcgcaaagtg gcgatgaggg aagtgtgatc gaagggcttc gagcccgccg
     3541 ccgggcgcag gatcgttccg ggccggatgt agggcgagac gatgacagcc gggacccgga
     3601 tgccgtagcg gtcaaatttg aagatctggc caggctgagg agtctcggga ggcacggcgg
     3661 ctggagggcc aacgtgatca taacagccac cgtgctcgtc gtaggtgatg acgagcagcg
     3721 tctcaggcca cgccggtccg ccgcgcacgc agttatagac gtcggcgatc aactgctcgc
     3781 cgagagtgac gacatgcggc ggatgctgat cgttaggcag ctccacgtcg ggaaaataac
     3841 gcggttcgat gaatgaatag gcgggcaggg cgccggaggc cgcatcatcg cgaaactgag
     3901 catacagtcg aaaccgttcc agatggcccc acaggtccgt cagcgtcaga cattgcggga
     3961 cttcgtggaa gtagatgcgc caggcatctg ccattccaac ctgatcgaac cgcgtgaaca
     4021 cggacggcat ccgatacgga aagtgcgccg gtgagttgtt ctcgtaacca ttggcggtgc
     4081 ccgtatgcac gaagaagcgg ttgggccagg tctgacaggg cgcggaggcg tgccattgat
     4141 cgcagaccgc aaacttcgtg gccagctcgc tgaggaccgg cacctgctcc ggagcgtaga
     4201 aatgcatcac ggcgcgtgga tcgtaggtcc ccgcaggtgt gccatccgcg gcctgagcgg
     4261 catagctttt gacgaatccg cccatcgagg ccgcagcgtc ctgcgccggc gaggcggtct
     4321 cgaagatctg ggtgttgatg tcggtgaaga gctcccctgg gtcaggcgct ggaattgcca
     4381 tgctgcgcgg atcctggccc gcagcattcc agacgctcac gcgcgcccca gccggatcga
     4441 ggttgaactc ctcgcccgtg aggccattga acgcctcaga tgccggataa agcttgccca
     4501 gcacactgtc gaacgagcgg ttctccagca tcaacacgac cacgtgctga atcttgtcca
     4561 tcagaatgtc catcctgcag ctccggcagg cctctgacgg cctcgcaggt ccacggggaa
     4621 agtatcccaa ggattctcgg tgtttgcagg ctgcctggat agcccgcaag ggcgcgctag
     4681 cttccgagcc gctcggtcca ggcttcgcag agctgcacgg cctccagcgg ctcgaaattg
     4741 tccggatcca gacaccactc gagccacaat ccgtcgatca gcgccgttag gccgatggcg
     4801 gccagtctca gattgaagcg cagctcgccc acttctcgtt ccaggtccgc cagcatgccc
     4861 cgcagcagat ccacatagcc gtggtaggtc tcgctgtgca cgcgctggat atccttcgag
     4921 tgccggtaaa gaccccagaa caccacccag acagccagca cgtcgctatc gagattgggg
     4981 ggcgagaacg acgccaggaa aaatgcgctg agacgggcac gcggagcgtg tggggcatcg
     5041 gcgaccgcct gccgcaggtt cgcgacgagc tcgttgttga agtagcggta cgcctcggcg
     5101 accagggcgc ccttggtcgg gaagtgatga ttgatcagcc cgatcgagac ctgcgcctga
     5161 gcgctgatgc ggcggatgga cagaccctcg tgcccgaacc gcttcagcga ttcgatcgtc
     5221 gcctcaatga gcgccttgcg gcgcgcctcc ggtagcttgc gctcgaagcg tggctcgcca
     5281 ttcttttgcc tggcgcgctt caatcaagcc gcccgcttga gccgcccccg tttgaaatcg
     5341 gcgaggatct cgcgcgcggc gttgtggccg ggcgcgccgg tcacaccacc gcccggatgc
     5401 gtgcccgcgc cgcacatgta caggcccggc agaggtccgc gatagtttcc atggcccagc
     5461 atcggtcgcg cggagaacat ctgatcgagc tgcagcgagc cgtgcatgat gtcgccgccg
     5521 accaggccaa aagtgcgctc gagatccagc gggctcatga tctgccgccc gagcacggat
     5581 gctttgaaat tcggcgcatg ctggttgacg gtctcgatca tcaggtcggc gacctgctca
     5641 cgatgtgcgt cccaggactc cccgcccggc agctccgctg ccacgtgctg gcagaagagg
     5701 ctcgccacgt gctgtcccgg agggctcaag gaatcatcga gcgtggaagg aatcagcatc
     5761 tcgacgatgg gccgcttgga ccagccgaag gtgcgggcat cgaagtaggc ctgctccatg
     5821 taacgcaaag tcggggccag aatgatgcct gcggtgtgat gttcgccgcg cgccttgccg
     5881 ggcaggcagg tgaaatccgg cagctcggac agggccacgt tcatgcgaaa cgtccccgag
     5941 ccgcagcgat agtgcgagat gcgcgcgcga aactcggcgg gaagtgccgc cggctccagc
     6001 agacccagat acagcagctt gggattgagg ttggaaatca ccgcggatgc ccggatgact
     6061 tctccggatg cggtgacggc gcccaccgcg cggccccctt ccaccacgac ctgctcgacc
     6121 ggcgtcgcga ctctgatctg cgcgccgcgc gagagcgccg actgggccat cgcctgggtg
     6181 atggcgccca ttccgccgat ggcgtgcccc caggctcctt tcttgccatt cacctcgccg
     6241 aagacatgat gcagcagcac gtaggcagag ccgggcgtgt agggactggc atagttgccg
     6301 acgatgccgt cgaaaccgta gaccgccttg atcggatcgc tctcgaacca gttatcgagg
     6361 tagtcgcccg cagaggtggc aaacaaactc aacagctcac gacgcagagc catgtcgagc
     6421 ttgcgcaagc gtccgcccac tcggcttgcc cgcagcaatt ccggcagcgc cgagcgccag
     6481 tcaccctgga cgacattggg aggggtctcg agcaccagat cgcgcaggac atcggcgacg
     6541 gcatccagac gctcgccata ggcatcgagg gccgcggcat ccttctggga gaagcgcgcc
     6601 acgtcagatc gggtgagcgc gcccccggtc ctgaggtatt ggccgtcctg cgtcggcagg
     6661 aagttggaaa cgagacgctc gaccacgcga agtccgtgac gcggcagctc gaggtctcga
     6721 atgaccttcg ggttcagcag cgacacggtg taggccgcaa ccgagttgcg aaaacctgga
     6781 tggaattcct cggtcaccgc cgctccgcct actacgccgc gacgctccag gaccgtgacg
     6841 cgcaagccgc ctgctgcaag gtaggcggcg caaaccagac cgttgtgtcc gccaccgatg
     6901 atgagcgcat cgcatggcct tgatgaatcc gacatcagtc tctccccgca gaaggccagg
     6961 cctggcgccg gttcaggcga cttcctgtgg aaggatctgg ggcgtgaagg ccggcagctc
     7021 cggggcggtg cgacgatcgc tccaaccctt cgcgggggca cgcagcagcc gcgtctcggg
     7081 aatcaacccg cgcatgcgcc gcccaaaact gcgcaggcaa acctcgtcct cgctgagtgg
     7141 cccgaccgta tagccgctcg tgctcatgcc cgactggacg cgctcgatca acgccttgtc
     7201 ctcgagactc acgcggcgat tgatccgcca gttgagatac cgtgcggctt tcatttcgcg
     7261 gcggctgtcc gggtggacgt acacgatctc gcggatcatg gtttcggtgg cggagaccgg
     7321 caggaactgc atgaagtcga tctgatccgg atagatgtcg aaggccacgt tgggccagag
     7381 cttgaagtag gtccacagcc gctggcgctc cgccggcagg tgctccacgg gcggcagcac
     7441 gctctgatag agcctctccg accagttgct ggaagggatc tcgcgcagct ctccccacat
     7501 cttgtcgatc cagggtttcg cttcgatccc atagccgcgg ccgaacaggc gagtcagtcc
     7561 cggatgggcg acgttgatgt gcaacccgtc cgagaagtta tccgcgacgt tcttccagtt
     7621 gaccatgcgc ggtcgcaggc tgacgcgacc gtgaggaacg agctcttcca tccggtatgg
     7681 agccagctca tgcgcatacg gtgccgccat ctcccgcacg ctgggcaggc cgggggcaaa
     7741 acggacgaag atgaagccga ggaaaacctc atgctctagg ggcacgagcc cgtgccgtgc
     7801 cttgtcgagg cccttgaagg cctcacgctg cgggatcgtc agaagtcggc catcgagtcc
     7861 gtaagtccac gcatgatagg ggcaggtgat ccggcgcccg caatggcccc gcggcccgtc
     7921 gagaagcttc gcagctcgat gacggcaggc attgtggaaa gcgcggacct cgccgtcctc
     7981 acctcgcaag acgatgacgg actcgccgag aaactcgagc gagtgatagt cccccgcctc
     8041 aggcacatcg ctttgatggc agacgatctg ccatgactga cggaagatca tctgcttttc
     8101 gagctcgaaa aactcggggt cgtgatagat ccaggccggc aggctgcagg cttcttcgtc
     8161 catagcggcg gattccagac agggtgctat tgaacgatcg tacaacaagc gaggcgccgg
     8221 ggcaaacacc cgggtccaag tgcgcgacgc acgccctacc ggctgaccag aaggccgacg
     8281 ggggttgctg tcagcagcca cgcaagggcg accgctgcag ccacggcggc gcaggcgacg
     8341 aaggcccagc gatagaatga gcccagcgct cggtcgcgcc ttccgccgcg gacgtcacgc
     8401 gcagcggtgg gggatccaac cgggtcagtc ggcgtggggg tgagccgcac ctgcgtcagc
     8461 aagtcggcgt cgaccgtgcc atccaggggg cgcaggttct tgagtgcatc gacacgatgc
     8521 caggcgactc tcagtcgcac gaacgcggcg cgatgacggg gatcggcgtc cagccaggcg
     8581 tgcagccctt gccagagctc cggggaggac tcgacctcca cgcgaatcag ccagttcgcc
     8641 gcctcgtccg agatccgatc tgcccggctc atgctcacag gtgaaggcgc aagcgggcga
     8701 gccatccctc cgtgggctgc agggccggag ggtcgaagac catctgcgcg cagcggcgcg
     8761 ccgcggcccg cagatcttcg agcaccagtc gcaccgggac cccgagccgt gaggcgatct
     8821 gagcggggga atagccgtac actttgcgca gcgtgaacac ttgacggcac ctttccggga
     8881 gccgctgaag cgcgctcacg agcctcgcca ggtcctggcg ccgttttacg atttcttcga
     8941 ccggctcacc cggctcgtcg gccgccaggg cttccagctc cgggccgatc gcgaccggct
     9001 gcgcgtgctc ttgccgcagg tagtccgcgg ccacatcgcc ggcgatgccg aacgcgaact
     9061 cgcggatcga gtgcacctca ggagcatccg cggcggcggc ggcaaggagg cgcgcgtagg
     9121 tttcctgcag gaggtcctcg acttcgcatt tctcaccggc ataccgatgc agacgcgctc
     9181 gcagcgagcc ctcgagctcg agcgcttgca ggaggtagcg ttcttcgttc ttcgacacga
     9241 ccgggacctc gcgctcattc gcctgccttc cgtgagagtc gctgacctgt tacccccgaa
     9301 agcgactcaa gttcattgag gccggcaata ttacgcacct attgcgcaca cggcgcaacg
     9361 gaaaaatacg tacggcatac tgggctcggt gccggactgt tggaagtgtg acccacaccc
     9421 ggtcaccggg gcgtgtctag ttcccgcctg cgcagtgcgc ctgccgggaa accttcacgt
     9481 ctgcaaggcc tggcgaacgg gaggctgctt cgcgaaacac tgtgccgcca cggatagtca
     9541 tcgttggctg tggctttggc ggattattcg cagcccgcgc gcttgcccgg gcagacgcgg
     9601 agctgctggt gat
//
LOCUS       DASRHN010000084         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1509834, whole genome shotgun sequence.
ACCESSION   DASRHN010000084 DASRHN010000000
VERSION     DASRHN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509834"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFB37_03200"
     CDS             complement(<1..1041)
                     /locus_tag="VFB37_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO21485.1"
                     /translation="MAAPLSDQLTYSATVNRSKALQILADAAIVVVIAGDAVPSVHLQ
                     ARNGSLDEIDQMLALVHGRRVLLGPLAAQVLADPQIYGGRFDAVHTHTVTSADLLGGS
                     RSAAAYERLAADRGAYAELIGQLGWRPVAEIELYRGCTRRRFCSFCNEPVKSPLVRFR
                     DPSDVLAEVTKLYEAGVRNFRLGQQTCFFSYLNRDADAIERLLAGVRERCPELEVLHI
                     DNADPLAVASRTGAKIARLVARYCTEGNCAPMGIESFDPVVIEANALSCTPEILLRAV
                     ANISDAGAHTGPRGLPVLLAGLNLVYGLPGETHRTHYENLSWLVRILDAGYLCHRINV
                     RQGHVYPGTPLSA"
     gene            1821..>2007
                     /locus_tag="VFB37_03205"
     CDS             1821..>2007
                     /locus_tag="VFB37_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21486.1"
                     /translation="MSLVIENAKICATSQSGSEQPRFADRRYLVVPAVDQQDRAADRV
                     RVDKHRSCAVIERRASLA"
BASE COUNT          379 a          650 c          630 g          348 t
ORIGIN      
        1 ggcagacagc ggcgtgccgg gatagacgtg cccttgccgg acgttgatgc ggtggcacag
       61 ataacccgcg tcgaggatgc ggaccagcca ggacaggttc tcatagtggg tgcggtgggt
      121 ctcgccaggc agcccgtaca ccaggttcaa gcccgcaagc agcaccggca ggcctcgcgg
      181 acccgtgtgt gccccggcat cgctgatgtt cgcgacggcg cgcagcagga tctccggtgt
      241 gcaggaaagc gcgtttgcct cgattacgac cgggtcgaag ctctcgattc ccatcggcgc
      301 gcagttgccc tcggtgcagt aacgggcaac cagccgcgct atcttcgctc cggtcctgga
      361 agcgaccgcc agtggatcgg cattgtcaat atgcagcacc tccagctccg ggcatcgctc
      421 ccggacgcca gccagcaacc gctcgatcgc atccgcatca cggttgaggt aagagaagaa
      481 gcatgtctgc tggccgagcc ggaagttccg gaccccggcc tcgtacagct tcgtgacctc
      541 agccagcacg tcggacggat cccggaacct caccagcggg ctcttgactg gctcgttgca
      601 gaacgagcag aacctgcgcc gcgtgcagcc ccggtaaagc tcgatctccg cgaccggccg
      661 ccagccgagc tggccgatca gctccgcgta cgcgccgcga tcggccgcca ggcgctcata
      721 tgctgccgcg ctgcggctcc cgcccagcag atccgcggag gtcaccgtat gcgtatgcac
      781 ggcatcgaac cggccgccat agatttgcgg atcagccagc acctgggccg cgagcgggcc
      841 gagcaggacc cggcggccat gtaccagcgc cagcatctgg tcgatctcgt ccagggatcc
      901 gttgcgcgcc tgaagatgca cagaaggtac ggcgtcgccc gcgatcacca ccacgattgc
      961 tgcatccgcc aggatctgca gcgccttact gcggttgact gtggccgagt aggtgagctg
     1021 atccgacagc ggcgcggcca catacggatt gccgccgtta aagcaccaac gcaggtcatc
     1081 aacggtcagg taccggacat cggcaccggg acaggcgcga ttcagcgcgc tgaatgcggt
     1141 ccgggcgtag gtggacaagt acggaggaac gccgagcccg ctcggctcca cggtatagca
     1201 gtcgatgatc gccgcaatct gcaccatcac gtctcctcgg ctgcggctat ctcggccgca
     1261 tatgctcccc tctcacggct ttacacaatg agtgtctgtc gaaatgcgcg tggaatcaac
     1321 gggcatgacg gcgttcttcc cgggttaacc atgagaacgg atggccgcct cctcctcgtg
     1381 gcgggaggcg gcagcaccgg ttgcgccgcg gggtcgggcc ttgcgtatcg gaatccgcga
     1441 cttctttgtc tgccattacg tcacctacac aagggcagac tcgatcagct cgaaacctgt
     1501 cacgcatcca caggatcttg gctctaccca cagcgctgcg cccgggacga tcttcgtgta
     1561 ctcggcacgg ttgccagaaa acgtagatat aggcgccgtg gaaaccgcga ggtcgctgcc
     1621 ggcaggatca ggccttcgct gagcacaaac ccaaagcgtg atctcatggc gaagcatggg
     1681 caatgcctag ttgtcctatt cggctaatga cactttggta ttggaggtgg gcttgattct
     1741 cattgttgcc gcagcgcagt tcgcgcaaag ccgagctcga gggctaggac ctcctcgagg
     1801 acttgcgagc ccctgaagcc gtgtccctgg tcatcgaaaa cgcgaagata tgcgcgactt
     1861 cccaaagcgg cagcgagcag ccgagattcg ccgaccggcg ttatctggtc gttcccgccg
     1921 tggatcagca agaccgggca gctgatcgcg tgcgcgtcga caagcaccga tcgtgtgcgg
     1981 taatcgagcg gcgcgccagt ctggccg
//
LOCUS       DASRHN010000085        10236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_357463, whole genome shotgun sequence.
ACCESSION   DASRHN010000085 DASRHN010000000
VERSION     DASRHN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357463"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFB37_03210"
     CDS             <1..296
                     /locus_tag="VFB37_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21487.1"
                     /translation="FLLQIQKSGLVPVIGLDWKDDDSQAREWLAQLGDPYEVVAVDRD
                     GRTAIDWGVYGAPETFLVNPQGVVVYKYVGPLTPGAWETEFVPRLPPHRAAKS"
     gene            296..814
                     /locus_tag="VFB37_03215"
     CDS             296..814
                     /locus_tag="VFB37_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HZO21488.1"
                     /translation="MRTPSARPPGVGLPAPRRGEGRGARARHFLLFALSAALLSGAPF
                     VAAVDTTELANPALQARYLALTHELRCMQCQNQTIADSPVGLASDMRREVREMLLAGK
                     SDEDVRNFMVARYGEFILFRPRFSVRNAWLWLAPGVLLVAGAIIGIRIVRQRNAMVGQ
                     DDTQVEEDSSPT"
     gene            811..1905
                     /locus_tag="VFB37_03220"
     CDS             811..1905
                     /locus_tag="VFB37_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21489.1"
                     /translation="MMVTFVLLAALFTVAGVALIAVPLLRPLRAEVAPAPWTALGVAG
                     VLVIGSALLYLAWSNWSWRAEPAADSPQTMVARLARKLERDPQDINGWLMLGRSYTVL
                     QQYPLAVRAYERADRLAGGKNVDALLGQAEALSLEDESELDRGAGRLVEQALLLEPNS
                     GKALFFGAAAAMRRGDLPLARARFAHLLSLNPPDNIKPLLQQQIAAIDQQLAAARGQG
                     GSSERGSVAADVAGKAQAAAASGTGASPSNGGSAPSVRINVTLAPSLAQAASADAPLF
                     VFVRDPQRPGPPLAVKRLLSHFPQTVELTASDSMMPGRAIVVGQKVQVVARIARSGNP
                     VGASGDPFGQIAYLVGHDGLSNIVIDQVTP"
     gene            1942..2829
                     /locus_tag="VFB37_03225"
     CDS             1942..2829
                     /locus_tag="VFB37_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZO21490.1"
                     /translation="MSGPVSLHFSSPPGIVPAYVRIFLSRKPSVVASDVPRIEAVLER
                     FVVNPRHLARYRKVCGDRVSDELPITYPHILATPVHLAMIASEAFPVSLLGVVHVRNR
                     IVQHRPLHVHDTGGIHAWMEGYRDTSRGQEIDLQTEVRIHDDVVWSETATFLARHRQR
                     RRLPRTGEPAMPNVEMPPRQDVTTSTFIVNRAVGRQYARVSGDFNPIHIADVGARFFG
                     LKRAIAHGMWSLARCAAEIGGGAFAQPCTLDVAFRRPVAFCARMVLESWMTDGRVGFS
                     LRDQDADRGHLLGTVVPTS"
     gene            2946..3341
                     /locus_tag="VFB37_03230"
     CDS             2946..3341
                     /locus_tag="VFB37_03230"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZO21491.1"
                     /translation="MAHAPTQDGRTSNRGFASMNQERQRQIASMGGKAVPDEKRSFSQ
                     NRRLAAEAGRKGGQSVPGSKRSFSQNRQLAAVAGRKGGQSVPDEKRSFSQNPELAAEA
                     GRKGGQASHGGGASGESQSRTRTPEQQEE"
     gene            3806..4474
                     /locus_tag="VFB37_03235"
     CDS             3806..4474
                     /locus_tag="VFB37_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21492.1"
                     /translation="MRLLLVEDDHMIGESLAKGLRDDGYVVDWVREGPAALAALRDQQ
                     AAYAIAVLDWNLPRQDGLSVLRTLRAEGNTLPVLMITARDELTDRISGLDSGADDYLI
                     KPFELGELKARLRSLLRRRDGRTTTLMVHADLVLDPVTHSVRQGEALIAVTPREFTLL
                     RALLERPGAVLSRSQLEERLYGWEDSVHSNAVEFTIHSLRRKLGAAAIENVRGVGWRL
                     SEPS"
     gene            4471..5862
                     /locus_tag="VFB37_03240"
     CDS             4471..5862
                     /locus_tag="VFB37_03240"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO21493.1"
                     /translation="MSSLRKRLTLWLWAAVALIAAISVLIGYWQAREETQDQLDYEMQ
                     QVVHILAGQSFSPAAAAPASAVPNISPTVHISHDEEDDLIVSVRDARGNLLYASRANR
                     QLSGSVLPSLDSLGFQMATIDGESYRVFVAQSGERRIEIAQSMDVIREAEEGIALATL
                     LPVLLLLPVLGLVIGLVIRRQLRPLNAAASLIASRPPLSLELLPLEDIPREVQPLVAE
                     INRLLGRLKTAVQREQRFVTDAAHALRTPLTALQLQADVLDGSRDPDEKAARLAQLRA
                     GIRRVSRLSEQLLSLARSESAAGPLTVSTELDSALEEIVAVHAAAAAARQITVRVESR
                     SRARVYGNARRLALIFGNLLDNAVRYTPDAGKVGVRAGVEANAAKVEVWDEGCGLPPE
                     ELERVFERFYRAPGDDSSGNGLGLATVEALLRQLGGRIVLANRVDRSGLIATVILPLA
                     SQLEAEEKEMAVS"
     gene            5859..7277
                     /locus_tag="VFB37_03245"
     CDS             5859..7277
                     /locus_tag="VFB37_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZO21494.1"
                     /translation="MSVERATRAESRRTPRLRLAGLAALGMALTGGCTFYHPKPLSSP
                     DVEASLESPDRAQLSRDALQLRHPRIQPLKLDFSRPLTGEEIEVIAVLANPDLRALRT
                     QQGVAEAQVFASGLLPDPQISLGYDKLLSPKNEGFSDAYAGSLTLDILGALATRHLER
                     QAAQATAEQLRNDIAWQEWTTAGQARLLALRLFYQRQASELAQQAASLAEHALQRALD
                     AAHSGDVSADDIEVRRVAASEATQRALSAARDAEGTRLDLNQTLGLKPEESVSLADPP
                     PLPSWTHLAPDELFARARIERLDLAALAQGYAGQEASLHRAVLGQYPRLGITINRASD
                     TSAVHTLGPAVTFDLPLWNRNRGAIATAKADRTRLRAEFAARLHQTRADIADLVDTLD
                     HDDSARAALAAQVPEMERIAKELQIAAARHDLAQPVAEAAASTALDKQITLLGLEQSC
                     AEERIALTLAIGSPVADVVEGP"
     gene            7274..8422
                     /locus_tag="VFB37_03250"
     CDS             7274..8422
                     /locus_tag="VFB37_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO21495.1"
                     /translation="MTARLMCALAIGLCVSGLIGCKKAGEESEPAPTASVVTQPAAQH
                     SVEQVLTAYGTVEFVPARTRALTVQVESQIAERFVLPGTVVKQGQALLRLIPSATSRL
                     DLDKAARDASVAVAEAQRIQRLQGQGLATDSELRSAKAAADTAVALRDSLVRRIGAAS
                     FEASSTRQAGSERGVTLTAPIAGVVDSLTAQPGDVIPPGTLLLRVSDPNALYVRLGIE
                     PQDAAEVHAGQAVVFSELFPRAPAQQGNIAEVDSRVDPQTRLTMVVVHPAPAAGLVPG
                     SSVRARIVIDTHEHALTVPRSAVLYDGDQPFLYVAAAGKAQRRLISVGITDGDLIEIL
                     RGLKAGEPVVTGGNYELQDGMSIRTAAAPESHGAAAGAAPAKSAPETP"
     gene            8419..>10236
                     /locus_tag="VFB37_03255"
     CDS             8419..>10236
                     /locus_tag="VFB37_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO21496.1"
                     /translation="MTDWLLAHQRSVLFLIAIAAIGGAVAAWHLPVALFPNIDFPRIV
                     VNLDAGDRPVDRMVLEITRPLEQALREVPDVGDIRSTSSRGTADLSLNFAWGTNMVAA
                     QLQVESAINRALPDLPPGIRFQVRRMDPTVFPIFGLTLTSDTRSLIDLHDLAFYTLRP
                     ILSAIPGVAQVEVLGGRVAEYQVVADPERLQASGLGIEAVAQALTANNTVSAVGRLED
                     RYRLYLTLADSRLQGAQDIENILLKSGPGGAIRLGDVASVRLSAAPQWTRVTANGHDA
                     VLINIRQSRGANSVELVRMVRAQMAEHARSVPAGVKIGTFYDQSELVSAAAASVRDAI
                     IIGAVLAGCVLLLFLRDVRLTVVVAIVLPVTLSITALLLASLGMSFNIMTLGGMAAAV
                     GLVVDDAVVIVEHLARRLHERGLEKASTDGGASLLPSAREMFKPLLGSSLATVVIFVP
                     LAFLSGVTGGFFKALAMTMACALVVSFFVALLGVPLLTDRFIQRYAARGEESGWFFHL
                     TERYARVSDAAMRHPGRIFLAALVLLIGGALALWKLPSGFMPHMDEGGFILDYRAPPG
                     TSLSETDRLLRKLEGLILAVPEVASYSRRTGLQLGGGITE"
BASE COUNT         1677 a         3366 c         3385 g         1808 t
ORIGIN      
        1 ccttcctgct gcagatacag aagtccggac tcgtgcccgt catcggcctg gactggaagg
       61 acgatgactc gcaggcgcgc gaatggcttg cacagctcgg cgatccctac gaggtggtcg
      121 cggtggatcg cgatggccgg accgcaatag attggggggt ttacggggcg cccgagacct
      181 tcctggtcaa tccgcaaggg gtcgtcgtct acaagtacgt gggtcccctg acaccgggtg
      241 cctgggagac cgagttcgtg ccgcgactgc cgccgcaccg ggccgcaaaa tcgtgatgag
      301 aactccgagc gctcgcccgc cgggtgttgg cttgcccgct ccgcgccgcg gtgaggggcg
      361 gggcgcccgt gcacggcact ttttactctt tgcactctcc gccgcgctgc tctcgggcgc
      421 ccccttcgtt gctgcagtcg atacgaccga gctcgccaat cctgcgctgc aggcgcgcta
      481 ccttgccctc acacacgagc tgcgctgcat gcagtgtcag aatcagacga tcgcggactc
      541 gcccgtcggt cttgcttccg acatgcgccg ggaagtgcgg gagatgctcc tcgccggcaa
      601 gtccgatgag gacgtgcgga acttcatggt cgcccgttac ggcgaattca tcctgttcag
      661 gccacgcttt tccgtccgca acgcatggct ctggcttgca ccgggcgtcc tgctggtggc
      721 gggcgccatc atcgggatcc ggattgtgcg ccagcgcaac gccatggttg gccaagacga
      781 tacgcaggtg gaagaggaca gctcacccac atgatggtga ccttcgtcct gctggcagct
      841 ctgttcaccg tcgcgggcgt ggcgctcatc gccgtccccc tgttgcgtcc gctgcgagcc
      901 gaagtcgctc cggcaccctg gaccgccctg ggggttgccg gcgtgctcgt gattggctcg
      961 gcgctgttgt atctggcatg gagcaactgg tcgtggcgcg cggagcccgc tgcggacagc
     1021 ccgcaaacga tggtcgcgcg gctggcacgc aagcttgagc gcgacccaca agacatcaac
     1081 ggctggttga tgctcggccg ctcctacacg gtgttgcagc aatacccgct ggcggtccgc
     1141 gcctatgaac gcgcggaccg cctcgccggc ggcaagaatg tcgatgcgct gctcggccag
     1201 gcggaggctc tctcgctcga agacgagagc gagctcgacc gtggcgcagg ccgcctggtg
     1261 gaacaggccc tgctgctcga gccgaattca ggcaaggcgc tgttcttcgg cgccgcagcc
     1321 gccatgcgtc gtggcgatct gccactcgcc cgcgcaagat tcgcgcacct gctcagcctc
     1381 aatccaccag acaacatcaa gccattgctg cagcagcaga tcgctgcgat cgatcaacaa
     1441 ctcgcggcgg ccagagggca ggggggctcc tcagagcggg gaagcgtggc agcagacgtt
     1501 gcaggaaagg cgcaggccgc agcggcgagc gggactgggg cgagcccgag caacggcggc
     1561 tccgcgccga gtgtccgcat caacgtcacg cttgcaccat cgctggccca ggccgcttcc
     1621 gcagacgctc ctctgtttgt gttcgtgaga gacccgcagc gtccggggcc accgctggca
     1681 gtcaagcggc tgttgagtca ttttccgcag actgtcgagc tcacggcttc cgattcgatg
     1741 atgcccggac gggcgatcgt ggtcggccag aaggtgcagg tcgtggcccg catcgcccgg
     1801 tccggaaatc cggtcggcgc gagtggcgat ccattcggtc agattgccta tctggtcggg
     1861 catgatggat tgagtaacat cgtgatcgat caagtcacgc cctgatcggc ccgcgagtgc
     1921 gttgatggta cagtcggctg catgtctgga ccggtctcgc tgcatttttc ctcgccgccg
     1981 ggaatcgtcc cggcgtacgt gagaatcttt ctctcgcgta agccatcggt cgtcgcctcg
     2041 gacgtgccgc gcatcgaggc agtgctcgaa agattcgtcg tgaatccgcg gcacctggct
     2101 cgttatcgga aagtctgcgg cgatcgcgtc tcggatgagc ttcccataac ttacccgcac
     2161 atcctcgcca caccggtaca tcttgcaatg atcgccagtg aggcctttcc cgtgagcctg
     2221 ctgggcgtcg tgcacgtacg caatcgcatc gtgcagcatc gacctctgca cgtgcacgac
     2281 acggggggca ttcacgcgtg gatggaaggc taccgcgaca cctcgcgagg gcaggagatc
     2341 gatctgcaga ccgaggtccg aatccacgat gatgtcgtgt ggtcggagac cgccaccttt
     2401 ctcgctcgac accggcagcg gcgccggctc cctcgaacgg gcgagcccgc gatgcccaat
     2461 gtggagatgc ctccgcggca ggacgtgacg acgagcacgt tcatcgtgaa tcgcgcggtc
     2521 ggccggcaat atgcgcgcgt ctcgggggac ttcaatccga tccatatcgc cgatgtgggt
     2581 gcgcgctttt tcggcctcaa gcgggccatt gcccatggca tgtggtccct ggcgcgatgc
     2641 gctgcggaaa taggcggggg tgctttcgcg cagccgtgta cattggacgt cgccttcaga
     2701 cggcccgtcg ctttttgtgc gcgcatggtg ctcgagagtt ggatgacgga cggtcgcgtc
     2761 ggtttcagtc tgcgtgatca ggacgccgac cgtggacatc tgctcggaac cgtcgtcccc
     2821 acgagctgaa atgcttgcat aaacctttca cgccgcgcgg tcttcaggcg gcatgggtct
     2881 tgctgggcta taggtgtgct cgctagtcga tgcgagcccg aagcatctat tggaggagag
     2941 tagtaatggc tcacgcacca acacaggacg gacgcacgtc gaatcgcggg ttcgcgtcga
     3001 tgaatcagga gcgccagagg cagatcgcca gcatgggcgg caaggccgtc ccggacgaga
     3061 agcgcagctt ctcgcagaat cgtcgtctcg ccgctgaggc cggtcgcaag ggcggccaga
     3121 gtgtccccgg ctcgaagcgc agtttctcgc agaaccgcca gcttgcggcg gtcgcgggcc
     3181 gcaagggcgg ccagagtgtt cccgacgaaa agcgcagctt ttcgcagaat cccgagctgg
     3241 ccgctgaggc cggtcgcaag ggcggccagg cgagccatgg cggcggcgct tccggggaaa
     3301 gccagtcccg cacgcgcact cctgagcagc aagaggaata gtcgaggcag tctgcactag
     3361 agggccgtcc agcggacagc ggacggcggc gacgtgggtt gcaaggatgc agcccatcag
     3421 ggtgttcccc gtccggggtt cacccttgag tcgtttccgc attgaaaaga ttaccttgga
     3481 ctttgcagat cttgcgcggc gcccgcgtcc gccgctgact tgagcgtcgg ccaagctttc
     3541 gcgcgcaggc ctcttctggg gcctcgggcg gcaacgggcg tttagctcat caagacgggg
     3601 cacacggcct cggcgcgacg atttgcgagc ccccccagca tgggtgagcc cgcgctttat
     3661 tcgggcagaa agcgcccacg gccagcagac ccggcctcga gccacggatc ccctaagtct
     3721 ctactaagtc ccaggctcca caattgcctg catgacctac catttccatc cctcgacctg
     3781 atgggctgcc ggagccgggt ccctgatgcg actgctcctt gtcgaagatg accacatgat
     3841 cggcgagagc ctcgcgaagg ggctgcgcga tgacggatac gtcgtggact gggttcggga
     3901 agggcccgcg gcgctggctg ctctgcgtga tcagcaagcc gcctacgcga tcgcggtcct
     3961 cgactggaac cttccacggc aggatgggct ctcggtgctc cgcacgctac gcgcagaagg
     4021 caacacgctg cctgtgctga tgatcactgc gcgcgatgag ctcacggacc ggatcagcgg
     4081 cctcgacagt ggcgcggatg actatctcat caagcctttc gagctcggtg agttgaaggc
     4141 acgattgcga agcctgctgc gcaggcgcga tgggcggacg acgacgctga tggtccacgc
     4201 ggacctggtg ctcgatccgg tgacccatag cgtgcgccag ggcgaggctc tgattgctgt
     4261 gaccccgcgg gagttcacgc tgctgcgcgc gctcttggaa cgtccgggtg cggttctctc
     4321 gcgatcccag ctcgaggagc ggctgtacgg ttgggaggac tccgtacaca gcaacgctgt
     4381 ggaattcacg atccacagtc tgcgcagaaa actcggtgcg gccgccatcg agaacgtacg
     4441 cggagtgggc tggcggttga gtgagccttc atgagctccc tgcgcaaacg tctcaccctc
     4501 tggctctggg cagccgtagc actgatcgcc gcgatctcgg tgctgatcgg ctactggcaa
     4561 gcccgcgagg agacgcagga ccagctcgat tatgagatgc agcaggtcgt acacatcctg
     4621 gcgggacaga gcttctcccc cgcggccgct gcccccgcat cggcggtgcc taacatctcg
     4681 cccactgtgc acatctcgca tgacgaggaa gacgacctga tcgtctcggt gcgcgatgcc
     4741 cgcggaaatc tcctgtacgc ctcccgtgcc aaccggcagc tctccggcag cgtgctgccg
     4801 tctctggact cgctcggatt tcagatggca accatcgatg gagagtccta ccgggtgttc
     4861 gttgcccagt cgggagagcg gcgcatcgag atcgcccaat ccatggatgt gatccgcgaa
     4921 gcagaggagg ggatcgcgct cgccacgctg ctgccggtcc tgctgctgct gccggtcctc
     4981 ggcctggtca tcggcctggt cattcgccgc cagcttcgcc cgttgaacgc ggccgcctcg
     5041 ctcatcgcca gccgtccgcc cttatcgctc gagctgctgc cgttggagga cattcccagg
     5101 gaagtgcagc cactggtcgc tgagatcaac cggttactcg ggcgcctgaa gacagcggtc
     5161 cagcgcgagc aacgcttcgt cacggatgcg gcgcacgcat tacgcactcc gctgacggcg
     5221 ctgcagctgc aggcggacgt cctggatggc tcgcgggatc cggacgagaa ggcggcccgc
     5281 ctcgctcaac tgcgtgccgg tatacgccgg gtgagccgac tgtcagagca acttctgtcg
     5341 ctggcccgaa gcgagtccgc cgccggcccg ctcacagtca gcaccgagct ggattcggcc
     5401 ctcgaggaga tcgtggccgt gcatgcggca gcagccgctg cacgccagat cacggtgcga
     5461 gtggagagcc gctcgagggc gcgcgtttac ggcaacgcgc gccgtctcgc gctgatcttc
     5521 ggcaatctct tggacaatgc ggtgcgctac accccggacg cgggcaaagt cggcgtgcgc
     5581 gcaggagtag aagccaacgc ggcgaaagta gaggtgtggg acgaaggctg cgggttgcct
     5641 cccgaagagc tcgaacgcgt gtttgaacgc ttctatcgcg cacccgggga tgacagcagt
     5701 ggcaacgggc tcgggcttgc gaccgtcgag gccctgctgc gacagcttgg tgggcgcatc
     5761 gtgctcgcga accgggtgga ccgaagcgga ctcatcgcaa ccgtgatcct gccgctcgcc
     5821 tcgcagctgg aggccgaaga aaaagagatg gcggtgtcat gagcgtggag cgcgccacga
     5881 gagccgaatc ccggcgcacg ccccgactgc ggctcgcggg gctggctgcg ctcgggatgg
     5941 ccctgaccgg gggctgcacc ttctatcacc cgaagccgct gtccagcccc gatgtggagg
     6001 cgagcctgga gtcgcccgat cgcgcacagc tgagccgcga tgccctgcag cttcgccatc
     6061 cccggatcca gcccctgaag ctcgacttca gccggccgtt gacgggcgag gaaatcgagg
     6121 tcattgcggt gctcgccaat ccggatctgc gggcactgcg cactcagcag ggagtggccg
     6181 aggcacaggt ctttgccagt ggcctgttgc ccgatccgca gatcagtctc ggctacgaca
     6241 agttgctctc acccaagaat gagggattct cggacgccta cgccggatct ctcacgctcg
     6301 atatcctggg cgccctggcg acgcgccacc tggaacgcca agcggcgcag gccaccgcag
     6361 aacagcttcg caacgatatc gcctggcagg agtggacgac cgccggtcag gcgcgcctgc
     6421 ttgcgctgcg gctcttctac cagaggcagg cgagcgagct cgcgcagcag gcggcgagtc
     6481 tcgccgagca cgcgctgcaa agggccctcg acgccgccca ttcgggcgat gtgtctgccg
     6541 atgacatcga ggtgcgccgc gtcgcagcct ccgaggccac gcaacgagcg ctgtcggcgg
     6601 cgcgcgatgc cgagggcacc cggctggatc tcaatcagac gctgggtttg aagcccgagg
     6661 agtctgtcag tctcgcggac cccccgccgc tgcccagctg gacccatctt gctccggatg
     6721 agctcttcgc gcgggcccgc atcgagcgac tcgacctggc ggcgctcgcg caaggctatg
     6781 cgggccagga agcctcactg caccgggcag tgcttggcca gtacccacgg ctcggcatca
     6841 ctatcaatcg cgccagcgac accagcgcgg tccacacgct gggcccggcg gtcactttcg
     6901 atctgcccct gtggaaccgg aatcgcggcg ccattgccac cgcgaaggcg gaccggacgc
     6961 ggctgcgcgc ggagttcgct gcccgtttgc atcaaacgcg cgccgatatc gcagacctcg
     7021 tggatacgct cgatcacgac gacagcgcac gggcagccct cgccgcacag gtaccggaaa
     7081 tggaacgcat tgcaaaggaa ctccaaatcg cagcggctcg ccacgacctc gcccagcccg
     7141 tggccgaggc cgccgcgtcg accgccctgg acaagcaaat cacgctgctc ggtctcgagc
     7201 agtcgtgtgc ggaagagagg atcgccttga cactcgccat tggctccccc gtcgccgacg
     7261 ttgtggaggg gccatgactg cacggctgat gtgtgccctc gccatcgggc tgtgcgtgtc
     7321 cggattgatc ggttgcaaga aggcaggcga ggagtcggag cccgcaccca ccgcgagtgt
     7381 cgtcactcaa cccgctgctc aacattcggt cgagcaggtg ttgactgcct acgggacagt
     7441 cgagttcgtg ccagcccgca cgcgggcgtt gaccgttcaa gtggaatctc agatcgccga
     7501 gcgattcgtg ttgcctggaa ccgtggtcaa gcagggtcag gctctgctgc gtctcattcc
     7561 cagcgccacc tcgcgtttgg atctggacaa ggccgcacgg gatgcttcgg tcgcggttgc
     7621 ggaagctcaa cggattcaac gcctgcaggg gcaagggctg gccacggact ccgagctgcg
     7681 ctcggccaag gccgccgccg acaccgcggt cgccctgcgg gacagtctcg ttcgccgcat
     7741 cggtgccgcc agcttcgagg cgagctctac acgccaagcc ggcagtgagc gcggcgtgac
     7801 gctcaccgca ccgatcgccg gcgtggtcga ttcgctgact gcccagccag gggatgtgat
     7861 ccctccgggc acgctgttgc tgcgcgtctc cgatccgaat gctctttacg tccggctggg
     7921 gatcgagccg caggatgccg ctgaggtgca cgccggacaa gccgtggtgt tctctgagct
     7981 gtttccgcgc gccccggcac aacagggcaa catcgcggaa gtggactcgc gcgtggaccc
     8041 ccagacgcgt ctcaccatgg tggtggtcca ccccgcgccc gcggccggcc ttgttccggg
     8101 gagttccgtg cgggcaagga ttgtcatcga tactcacgag cacgcgctga cggtgccgcg
     8161 atcggcggtg ctgtacgacg gcgatcagcc ctttctgtat gtggcggcag caggcaaggc
     8221 tcagcggcgg ctcatctccg tgggcatcac cgacggggac ttgatcgaga tcctgcgcgg
     8281 cctgaaggcc ggtgagcccg tcgtcaccgg gggcaactac gagctgcagg acggaatgtc
     8341 gatccggaca gccgcagcac cggagagcca cggcgccgct gccggggcgg cccctgcgaa
     8401 gagtgctccc gagacaccgt gaccgactgg ctcctcgcac accagcgctc ggtgctcttt
     8461 ttgatcgcga tcgccgcgat cggcggggcc gtcgccgcct ggcacctgcc ggtcgcgctc
     8521 ttccccaaca tcgattttcc gcgcatcgtg gtgaatctcg atgcgggcga caggcccgtc
     8581 gatcgaatgg tgctggagat cacccgaccg ctggagcagg cgttgcggga ggtgcccgat
     8641 gttggggata tccgctccac gagctcccgg ggcaccgcgg acttgtcgct caactttgcc
     8701 tggggcacca acatggtggc cgcccagctg caagtggagt cggcgatcaa ccgggcgctg
     8761 ccggacctgc ccccgggaat ccgcttccag gtcaggcgca tggatccgac cgtctttccg
     8821 atcttcggtc tgacgctcac ctccgatacg cgcagtctca tcgacctgca cgatctcgcc
     8881 ttctacacgc tgcgccccat cctgtcggcc attcccgggg tcgcgcaggt cgaggtgctc
     8941 ggaggtcgcg tcgcggagta ccaggtcgtc gccgatccgg aacggctgca ggcctccggg
     9001 ctcggtatcg aggcggtggc ccaagcgctg acggccaaca ataccgtgag cgccgtcggc
     9061 cggctcgaag atcgctatcg cctctatctg actctcgccg acagccggct acagggcgcg
     9121 caggacatcg agaatattct gctgaagagc ggtcccgggg gcgccatccg actgggcgac
     9181 gtcgccagcg ttcgactgag cgctgcgccg cagtggacgc gggtcacggc aaacggtcat
     9241 gacgcggtcc tcatcaacat ccgccagtcg cgcggggcca actcggtcga gctggtgagg
     9301 atggtgcgcg cgcagatggc tgaacacgcg cgctccgtgc cagcgggcgt caagatcggc
     9361 accttctacg atcaatcgga gctcgtgtcc gcggcagccg cgagcgtgcg cgatgccatc
     9421 atcatcggtg ccgtgctggc cggttgcgtg ctgctgttgt tcctgcggga tgttcgcttg
     9481 acggtggtcg tcgcgatcgt cctacccgtg acactgagca tcaccgctct gctgcttgcg
     9541 agcctcggca tgagcttcaa catcatgacc ctcgggggca tggccgccgc cgtcggtctg
     9601 gtggtcgatg acgccgtggt catcgtcgag catctggcac ggcgcctgca cgagcgcggc
     9661 ctcgaaaaag catccaccga cggtggcgct tcgctgctgc cctctgcacg ggaaatgttc
     9721 aaacccctgc tcggatcctc cctcgcaacg gtggtcatct tcgtgccgct cgcctttctc
     9781 tcgggagtga ccgggggctt cttcaaggca ctggcgatga ccatggcctg cgcgctcgtc
     9841 gtgtccttct tcgtggcgct cctcggagtg cccttgctga cggatcgatt catccagcgc
     9901 tatgcagcgc ggggcgaaga gtcgggctgg ttcttccatc tgaccgagcg ctacgcgcgc
     9961 gtgagcgacg cggcgatgcg ccacccgggc cgcattttcc tggcggcgct ggtgctgctg
    10021 atcggcggtg cgctcgccct gtggaagctg ccctcgggct tcatgcccca catggatgag
    10081 gggggattca tactcgatta ccgtgctccg cccgggacct cgctctcgga gaccgatcgg
    10141 ctgctgcgca agctggaagg actcatcctg gcggtcccgg aggtcgcaag ctactcacgc
    10201 cgcaccggac tgcagcttgg aggcggcatc acggag
//
LOCUS       DASRHN010000086         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2172166, whole genome shotgun sequence.
ACCESSION   DASRHN010000086 DASRHN010000000
VERSION     DASRHN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2172166"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFB37_03260"
     CDS             complement(<1..936)
                     /locus_tag="VFB37_03260"
                     /inference="COORDINATES: protein motif:HMM:NF005762.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HZO21497.1"
                     /translation="MVDYIGVERVRELVTHIGPARFVEALAAEIEADYRRWPQFEKCP
                     RHATHSPGGVIELMPTSDGRLYSFKYVNGHPKNTSDGLLTVTAFGVLSDVDTGYPLLL
                     SELTFTTALRTAAMSALAARHMARPSSRVMALIGNGAQSEFQAIAFHQMLGIRELRLH
                     DTDPLAMLKLERNLAELHLQGLKVIRCASAAEAVRGADIVTTVTADKRNALILTPEMI
                     APGMHLNAVGGDCPGKTELHADILRRADARVIVEYEPQCRIEGEIQQLEPSFAVTELS
                     EVLCGRASGRVADHEVTIFDSVGFALTDFSALRYLY"
     gene            1076..1498
                     /locus_tag="VFB37_03265"
     CDS             1076..1498
                     /locus_tag="VFB37_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO21498.1"
                     /translation="MDDTDRQLLGLLRDNARTSIATLARELRVARGTVQNRLQRLEAD
                     GTIVGYTVRLRPQEEAQRIRALTTVAVEGNRTDEVLRALRGDPAVGALHTTNGRWDIV
                     AELRADTLEAFDRVLGRIRQIEGISSTETSLLLSTHKL"
     gene            complement(1584..2057)
                     /locus_tag="VFB37_03270"
     CDS             complement(1584..2057)
                     /locus_tag="VFB37_03270"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZO21499.1"
                     /translation="MLNLRDRLYVCVGVMLLALMPMVGNSADGAPTKEEVVAQVKKAV
                     AFYKANGREKTLAEMNNKDGQFAKGEDYIDVHDIHGVCQAHPISPGVVGLNRMDAADP
                     AGKKWIREIVEETRAGKTSGWSTYQRKNPVSGKIEHKLAYWELSDDLIFKAGTYE"
     gene            complement(2084..>2719)
                     /locus_tag="VFB37_03275"
     CDS             complement(2084..>2719)
                     /locus_tag="VFB37_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZO21500.1"
                     /translation="QCAETGNAVVAKAVEAMGSINTASRKIADIIGVIDEIAFQTNLL
                     ALNAAVEAARAGEQGRGFAVVASEVRTLASRSAEAAKEIKGLIKDSVVRVAAGERVVT
                     ESGATFEQVVIAVKKVGEIIAEIAAASAEQAAGIDQVGTAVSKMDRLTQENAALVEEA
                     AASSQVLADQASSLSGLMRRYRVGTEAGAASGVSESIERRSAATQWTQRTA"
BASE COUNT          471 a          910 c          840 g          498 t
ORIGIN      
        1 atacagatag cgcaacgccg agaaatccgt cagcgcaaat cccaccgagt cgaagatggt
       61 gacctcgtgg tcggcgaccc gaccagaagc tctgccgcac agcacttccg acagctcggt
      121 cacggcaaag gaaggctcga gctgctggat ctcgccctcg atgcgacact gaggctcgta
      181 ttcgacgatc acgcgcgcat cggctcgccg cagaatatcg gcgtgcagct cggtcttgcc
      241 gggacagtcg ccgccgaccg cattcagatg catgccgggc gcaatcatct cgggcgtcag
      301 aatcagcgca ttgcgcttat ccgccgtcac ggtggtcacg atgtccgcgc cccggacggc
      361 ctccgctgcc gaggcgcaac gaatgacctt caaaccctgc aggtgcagct cggcgagatt
      421 gcgctccaac ttgagcatcg cgagcggatc cgtgtcgtgc aagcgcagct cgcggatgcc
      481 gagcatctgg tgaaaggcga tcgcctggaa ttcgctctgg gcaccgttgc cgatgagcgc
      541 catgacgcgg ctggaggggc gcgccatgtg gcgcgcggcc agagcagaca tggccgcggt
      601 tcgcaaagcc gtcgtgaagg tcagttctga cagcagcaac gggtagccgg tgtccacgtc
      661 cgagagcacg ccgaaggcgg tcacggtcag caacccgtcg ctcgtgtttt tcgggtggcc
      721 gttgacgtac ttgaacgaat acagccgccc gtcgctggtg ggcatcagct cgatcacccc
      781 accgggcgaa tgagtcgcgt gtcgagggca cttctcgaac tgcggccagc gccggtagtc
      841 cgcctcgatc tcggccgcga gggcctccac gaaacgcgca ggtccgatat gggtgacgag
      901 ctcccggacg cgctcgacgc cgatgtagtc caccatgatc gtatccccag ctgttgctct
      961 gtgcggctag tgtcgccagc gctcgcgcca atgaatagcc agaaaattga tcaaaaagct
     1021 gtcaagtatg gtcaaaagta cagctttggc tggcgttttg gctagaatag cagccatgga
     1081 tgacaccgat cggcagctgc tcggattgct ccgtgacaac gcccgaacct cgatcgcgac
     1141 gctggcgcgg gagctgcggg tcgcacgggg aacggtgcag aaccgcctgc aacggctcga
     1201 ggcggacggc actatcgtgg gctacaccgt ccggctgcgg ccccaggagg aggctcagcg
     1261 gattcgcgcc ctcaccacgg tggccgtcga gggcaatcgg accgatgagg tgttgcgtgc
     1321 cttgcgcgga gatccggcag tgggtgcctt gcacaccact aacgggcgct gggacatcgt
     1381 ggcagagctg cgcgcggata cgctcgaagc ctttgaccgg gtgctgggac gcatccgtca
     1441 gatcgagggc atctcgagca ccgagaccag cctgctgctc tccacacaca agctctgatg
     1501 tgagctggcg gcggcgcggc gaacacgccg cgacgccgtg agcctatcga cagaaagggc
     1561 gcgccggatc cccggcgcgg ccgttactcg tacgtgccag ccttgaagat cagatcgtcc
     1621 gagagctccc agtaggcgag cttgtgctcg atctttccgc tgaccgggtt cttgcgctga
     1681 taggtgctcc agccactcgt cttgcctgcc cgggtctcct cgacgatctc gcggatccac
     1741 ttcttgcccg caggatccgc cgcatccatg cgattcaatc cgacgacgcc tggggagatg
     1801 ggatgggctt ggcagactcc atggatgtcg tgcacatcaa tgtagtcctc gcccttggcg
     1861 aactgcccgt ccttgttgtt catctcggcg agggtcttct cgcggccatt cgccttgtag
     1921 aaggcgaccg ccttcttcac ctgagcgacg acctcttcct tggtgggcgc gccatccgca
     1981 ctgttgccaa ccatgggcat gagtgcaagc agcatcacgc ccacgcacac gtacaatcga
     2041 tcacgcaaat tcagcatatg ttgtcctcca gaatctcgtt cactcaggcg gttcgttgcg
     2101 tccactgggt ggccgcgctc cgccgctcga tgctttccga tacgccgctc gccgcaccgg
     2161 cttcggtgcc gacgcgatag cgtcgcatca atccgctcag gctgctcgcc tgatccgcaa
     2221 gcacctgcga ggaggcggcg gcctcctcca ccagtgccgc gttctcctgg gtcagccggt
     2281 ccatcttgga gaccgccgtc ccgacctgat cgatgccggc ggcctgctcg gcgctggccg
     2341 cggcgatttc cgcgatgatc tcgccaacct tcttgactgc gatgacgacc tgctcgaagg
     2401 tcgcgcccga ctcggtcacc acgcgctcgc ccgctgcgac gcgcaccacg ctgtccttga
     2461 tcagaccctt gatctccttg gcggcctccg cgctgcgcga ggccagcgtg cgcacttcgc
     2521 tggccaccac cgcaaagccg cgcccctgct cgccggcgcg agcggcctcg accgccgcat
     2581 tcagcgccag caggttggtc tggaaggcga tctcatcgat caccccgatg atgtccgcga
     2641 tcttacgcga ggcggtgttg atcgagccca tggcctcgac ggctttcgca accaccgcgt
     2701 tgccggtctc cgcacactg
//
LOCUS       DASRHN010000087         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2172653, whole genome shotgun sequence.
ACCESSION   DASRHN010000087 DASRHN010000000
VERSION     DASRHN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2172653"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1659)
                     /gene="betC"
                     /locus_tag="VFB37_03280"
     CDS             complement(133..1659)
                     /gene="betC"
                     /locus_tag="VFB37_03280"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HZO21501.1"
                     /translation="MGQTPNLLILMADQLAPRMLPAYGGSVARTPHIDALARAGVVFE
                     SFYCNSPLCAPSRFSFMAGQLPSKIGAYDNAAEFPAQVPTFAHYLRAAGYQTALSGKM
                     HFCGPDQLHGFEDRLTTDIYPADFGWTPDWSRFAERPGWYHTMESVITAGACTRTNQI
                     DFDDEVVAAARQKLFDIARSKDSRPFCLVVSMTHPHDPYVIPTAYWNRYRDEEIDLPR
                     VPDLAGREDPHSTRLRHVIGLDLAKPSTAQIRAARRAYCGAVSYVDDQIGSMINTLKE
                     AELDRNTVTVLLADHGDMLGERGLWYKMSFYEGACRIPLIVHAPGRFAPRRVTAVASL
                     LDLLPTFAALANGGAEPAYAAPIDGHNLLPQLAANAPERNAAEVVGEYLAEGAIAPII
                     MIRRGRHKYVHSPADPDQLYDLESDPDERKNLATDLGFAEAIAQFRTEVARRWSLPEL
                     HEAVLASQRRRHFVYTTLRQGEQHPWDFQPRRDASRLYIRNDQELNDLERMARFPRLA
                     "
     gene            complement(1750..1953)
                     /locus_tag="VFB37_03285"
     CDS             complement(1750..1953)
                     /locus_tag="VFB37_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21502.1"
                     /translation="MQAIAAIADRQRLETYPFYSAALNEFELRSGHRSRARQHFRSAL
                     ALARNATERRFLEHRLAACDCRN"
BASE COUNT          353 a          639 c          704 g          368 t
ORIGIN      
        1 ttcgtaacca ttgtgctgga tcattgccag gaggtgacga tcggcgatgt tgccggggtc
       61 catgccaggg atcgcccggt gcaccgtaag gggctcagcc gcgccctgca agccgcgcat
      121 gcgtccgaga tctcaggcca gccgcggaaa gcgcgccatg cgttcgagat cgttgagctc
      181 ctggtcattg cggatgtaga ggcggctggc atcgcgcctc ggctggaagt cccagggatg
      241 ctgttcgccc tgccgcaggg tcgtgtacac gaagtggcgt cgtcgctgac ttgccagcac
      301 cgcctcatgt aactcgggca gcgaccaccg gcgcgccact tccgtacgaa actgcgcaat
      361 cgcctcggcg aagccgagat cggtggccag attcttgcgc tcgtccggat cactttcgag
      421 gtcatagagc tggtcaggat ctgccggcga gtggacatac ttgtgccggc cgcgccggat
      481 catgatgatc ggtgcgatgg ccccttcggc cagatactca cccaccacct cggcagcatt
      541 gcgctccggt gcgttcgccg cgagttgcgg cagcagattg tgcccgtcga tcggcgcggc
      601 gtaggctggc tcagcgcccc cgttggcgag cgctgcgaag gtgggcagca gatcgagcag
      661 cgaagccact gcggtcacgc gccgcggcgc aaagcgtccc ggcgcgtgca cgatcagtgg
      721 aatcctgcag gccccctcgt agaagctcat cttgtaccac aggccgcgtt cgcccagcat
      781 gtcaccgtga tcggccagga gcaccgtgac cgtgttgcga tccagctccg cctccttgag
      841 cgtgttgatc atcgagccga tctgatcgtc cacgtacgag accgctccgc agtaggcgcg
      901 tcgggcggcg cggatctgtg ccgtgctcgg tttcgcgagg tcgagtccga tgacgtgacg
      961 caggcgcgtc gaatgcgggt cctcgcggcc cgcaagatcg gggacccgcg gcaggtcgat
     1021 ctcctcgtcc cggtagcgat tccaataggc cgtgggaatg acgtagggat cgtgcgggtg
     1081 ggtcatcgag accaccaggc aaaaaggccg cgagtccttg ctacgtgcaa tgtcgaagag
     1141 tttctggcgt gccgcggcca cgacctcatc atcgaagtcg atctgattgg ttcgggtgca
     1201 agccccggcc gtgatcacag actccatcgt gtgataccag ccaggacgct cggcgaagcg
     1261 gctccagtcc ggggtccagc cgaagtccgc cggatagatg tccgtggtca gacgatcctc
     1321 gaagccgtgg agctggtcgg gcccgcagaa gtgcattttg ccgctcagcg ccgtctgata
     1381 gccggcagcg cgcaggtagt gggcaaacgt ggggacctgc gcgggaaatt ccgcggcgtt
     1441 gtcgtaggcg ccgatcttcg agggcagctg tccggccatg aaggaaaagc gcgacggcgc
     1501 gcacagcggg ctgttgcagt agaaggattc gaacaccaca ccggcccgtg ccagcgcatc
     1561 gatgtggggt gtgcgcgcca cgctgccccc gtatgcgggg agcatacgcg gcgcgagctg
     1621 gtccgccatc aggatcaaca gattgggggt ctggcccatg cgagctcgaa gtatacctgg
     1681 gtgcgatccc tcgccacgct tcggttgccg cgcgagcgtg ccgttgaacg cctgcgacgc
     1741 tggcaccggc tagttgcggc aatcgcacgc agcgagtcga tgctcgagga agcgccgctc
     1801 ggtcgcattg cgcgccaggg cgagtgctga gcggaaatgc tgccgggccc gcgagcgatg
     1861 accgctgcgc agctcgaact cgttcaacgc ggcgctatag aacggatagg tctcgagacg
     1921 ttgacgatcg gcgatcgcag cgatcgcctg catgctcggg gccctcgagc tgcgccagcg
     1981 ttgtgaagac aacctacgac gcttttggga ggccgggcgc ggacaatttt tttagcgtag
     2041 ctcgcgcaag gctttcgcgt cagg
//
LOCUS       DASRHN010000088        14627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1018392, whole genome shotgun sequence.
ACCESSION   DASRHN010000088 DASRHN010000000
VERSION     DASRHN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018392"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFB37_03290"
     CDS             complement(<1..326)
                     /locus_tag="VFB37_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21503.1"
                     /translation="MSADHRSETQWKLAALLAVLSMVSPFSIDTFFPSFHTIAAHFHL
                     SRWAVQQTLTVYMLPLAVMSLVQGPLSDALGRRPVILAGLATYTAASIGCTLAPNFGS
                     LLVFRAL"
     gene            complement(323..1117)
                     /gene="trpA"
                     /locus_tag="VFB37_03295"
     CDS             complement(323..1117)
                     /gene="trpA"
                     /locus_tag="VFB37_03295"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZO21504.1"
                     /translation="MKARDVISQAIRAASAGGEPALVAFLTAGFPSRERFREHLHAVA
                     DGADVVEIGVPFSDPMADGVTIQRSSEVALSQGVSLRWILKELEAMPAASAPLLLMSY
                     LNPLLAYGLDELAQASVRARVSGFIVPDLPYDEGAELRQALDAHGIAMVQMVTPVTRA
                     QRMQQLCESSQGFVYAVTMTGTTGKNVAVPPEVMGYLDRVRSLAPVPVCAGFGIRTRE
                     QVQRLRGHVDGVVIGSALVEVLEREEAPTQWLRALRATAPEARETG"
     gene            complement(1117..2367)
                     /gene="trpB"
                     /locus_tag="VFB37_03300"
     CDS             complement(1117..2367)
                     /gene="trpB"
                     /locus_tag="VFB37_03300"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZO21505.1"
                     /translation="MTPAIDAERLLADSMAGKFPDARGRYGPFGGRYVPETLIPALER
                     LEGGIAEHLHARLFQSELQHQLRTWVGRPTALTFAARLSERWGAEVWLKREDLAHTGA
                     HKINNAIGQALLAKRLGAKRIVAETGAGQHGVASAAACARLGLPCTVYMGSVDMERQA
                     PNVGRMRLLGAIVVAVTTGDRTLRAAIDEALRDWVSDPLDTYYLIGSAVGPHPYPYLV
                     RELQAVIGREARAQMLEETGSLPDVVIACVGGGSNSIGAFHAFVPDRSVAIIGVEAGG
                     QGRSLGSNAATLSYGSPGVLQGSFSMLLQDENGQIQETHSVSAGLDYPGVGPEHALLL
                     ATGRAAYETASDEEALEAVRACSECEGILPALESAHALVGARRWAQRNPGRRILVCLS
                     GRGDKDMSTLQSTLLANKATSEQP"
     gene            complement(2367..2987)
                     /locus_tag="VFB37_03305"
     CDS             complement(2367..2987)
                     /locus_tag="VFB37_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZO21506.1"
                     /translation="MTWIKICGMTTPAAVDTALETGVDAIGFVFAPSPRQLTPQAAAG
                     LAGPARGRIACIAVMRHPTQDSLEEILERFRPDALQTDLEDLRTLQIPQQLEVLPVIR
                     LWQDEGRQPRGRVLFEAADSGVGMRADWDGASRLARQVELVLAGGLTSANVVTAMTAV
                     RPFGVDVSSGVERERAIKDPAEIRRFVSAVRAHDQAGSPHVEEKSR"
     gene            complement(2984..3841)
                     /locus_tag="VFB37_03310"
     CDS             complement(2984..3841)
                     /locus_tag="VFB37_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HZO21507.1"
                     /translation="MSGDSGFLKQMAASSRERAARARARVTEAALLERALASGPAPAL
                     RASPEGFDLIAELKLRSPAEGQLRPVSEDVGARVEVYAGAGAAAVSILTEPSRFDGSM
                     AHLELAARALRPLQVPAMRKDFLVDPYQVIEARVAGAGGVLVILRMLPVAQIHSLMDC
                     ARQLGMFVLLEAFDEHDIEIMHDILQSHAAHSGAAGTPAPVLAGLNCRDLSSLQIVPE
                     RLLELVDLLPAGVPRVAESGVRSPEDARRVAAAGYRYMLVGSALMQTDPHVLAEALLS
                     AGRAAMGSS"
     gene            complement(3838..4884)
                     /gene="trpD"
                     /locus_tag="VFB37_03315"
     CDS             complement(3838..4884)
                     /gene="trpD"
                     /locus_tag="VFB37_03315"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZO21508.1"
                     /translation="MREMLEILLDRRDLAESQAEELLTCLTSAQCPPPMAGAILAALS
                     SKGVVADEVRGFARGMRRLARRPQVPAGLRAIDVVGTGGDASGSLNISTGTALLAAAC
                     GLPVLKHGNRSVSSRAGSADVLEALGLEIPLDEQAAGECLAACNFTFLFAPHYHPAMK
                     AVAPVRAALGVRTIFNILGPLTNPGEPPFHLIGAYSREVAQLIAQTLAGMEVERSFVV
                     HGAHGWDEPTPAGPFELFDVRPGRIVAEVREPQDYRLSRCTAEGLAGGDAQHNARALR
                     AVLMGEDRGPHRDCLLLGAALALEVTGQARTPHEAVERAVEAIDSGASRRVLESLAAF
                     SAARGASRPTALPV"
     gene            complement(4877..5491)
                     /locus_tag="VFB37_03320"
     CDS             complement(4877..5491)
                     /locus_tag="VFB37_03320"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZO21509.1"
                     /translation="MKPQVLLIDNYDSFTYNLVQAFLVLGADVEVHRNDAITPQQARD
                     LSPSHLCISPGPGTPADAGVSMDMIRAFAGRIPVLGVCLGHQSMVEVFGGKVVRAGRL
                     MHGKTSIVHHDERTVFAGLPQACEVGRYHSLIAELESLPGELEISARTLEGEIMGVRH
                     RELVLEGVQFHPESILTPQGPRLLENFLRLTGGLRPDFAARCHA"
     gene            complement(5488..6888)
                     /locus_tag="VFB37_03325"
     CDS             complement(5488..6888)
                     /locus_tag="VFB37_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HZO21510.1"
                     /translation="MKPPFDIAGDLDTPVSAFIKLAAFAPRFLLESVEGGERLGRYSF
                     IGFGDGLEVRLDRDALFIGGERRPAPADGAALLAALREALRRAPQPRPDIAGVPLAGG
                     LVGYAAYDVVRSFERVSAPTLSGVPLLHYVAPCSLLVFDHLTRGIALLHAGSEQERRS
                     LRTEIISALRGALPGAGRRVGRYSQPTASYARADYMEGVRRVQEYIAAGDVYQLVLSA
                     RFEGRHEIEPFQAYRALRLINPSPYMYYCALGDVTVVGSSPEALVKLKDGHAQLRPIA
                     GTRPRSDELSTDLAREAELRADPKENAEHVMLVDLARNDLGRVARPGSVRVDPYRAIE
                     RYSHVMHIVSGVHGLLEDSRDAFDLFAAAFPAGTLVGAPKVRAMQIIAELEPTNRGLY
                     GGTVGYFGARGDMDHAITIRTLVFNADRYSFQAGAGIVADSVPETEYEEVLAKSAAMG
                     RALEMTCDASAGGGGL"
     gene            7146..7490
                     /locus_tag="VFB37_03330"
     CDS             7146..7490
                     /locus_tag="VFB37_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003154107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin assembly protein"
                     /protein_id="HZO21511.1"
                     /translation="MSFKKLLEGWRESAAAPRTAAEYAVRLPVDDAARLRALADMFPG
                     RSTEQLITDLLGTALQEIAAAMPYVAGHKVISTDDQGDPVYEDVGPTPRFMELARRHR
                     RKLEAELKKAAG"
     gene            7501..7983
                     /locus_tag="VFB37_03335"
     CDS             7501..7983
                     /locus_tag="VFB37_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZO21512.1"
                     /translation="MPSFDVVSELNIHELSNALDQANRELAQRFDFKDSGAVFELKEF
                     TVTLRAQVDFQLKQMLEILKLRLTKRGIDLACLEVKEPQTTLASAQQEVVLKHGIDQE
                     TGKKIMRRMKDSKLKVQAALQADKVRVTGKQRDELQQAIQLLRAEKLGVPLQFNNFRD
                     "
     gene            complement(7980..8879)
                     /locus_tag="VFB37_03340"
     CDS             complement(7980..8879)
                     /locus_tag="VFB37_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZO21513.1"
                     /translation="MNSPPPAARRALAAVLVWALATAPLPAADPPVTVTDDAGQTLSL
                     PHHPQRIVSLTPGATEMLFAAGAGAQVIATVEYSDEPAAARRVPRIGDVVAIDMERLV
                     ALRPQVAVIWPGGGNPAQIEQIRRMGIPEYSQQVNSLADLPGSLRRLGQLAGTSAIAE
                     PAARDLEARVARLRRTYGAGPHPTVLLQIWDHPIYTVGGTQLMSDALQLCGARNAFGD
                     LRQLAPAIDVEAVIARDPDVIVAAAPPGNAAQWLADWKRFATLRAVRTGHLIAFEDRR
                     LTRLGPSLLDATEGLCQALAALH"
     gene            complement(8876..9334)
                     /locus_tag="VFB37_03345"
     CDS             complement(8876..9334)
                     /locus_tag="VFB37_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZO21514.1"
                     /translation="MSSSTSATDTNEKIVTILNRILELELAGLVRYLHYSFMIFGHNR
                     IPIVGWLRAQADESQAHAVKAGEHITNLGGHPSLRIGSLLETHKHNVDEILGEAHAHE
                     EEGLAEYRALLELVAGKSIMLEEYARLQIATEEEHLSEIRKMMRKPGSIK"
     gene            complement(9425..9841)
                     /locus_tag="VFB37_03350"
     CDS             complement(9425..9841)
                     /locus_tag="VFB37_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZO21515.1"
                     /translation="MSARLSAGVIVVRNAREAWRFLMLRAYRNWDFPKGLVEAGEQPL
                     AGALREVQEETLLADLQFTWGEAYVETAPYSHKKVARYYIAETATEAVTLPVNPQLGR
                     PEHNEWRWVSFEQGLSLCSPRLDPILRWAAATIGIR"
     gene            complement(9906..10469)
                     /locus_tag="VFB37_03355"
     CDS             complement(9906..10469)
                     /locus_tag="VFB37_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZO21516.1"
                     /translation="MTTLDTIRDAIPEYARDLRLNLGSVLASTGAPGLSEQQIWSVAV
                     AAAIASRNLTFSTDIEALSSSHLDAAHLNAARAAAAIMGMNNVYYRFLHLVEDPEYAQ
                     LPARLRMNVIGNPGIDKQDFELLSLAVSAINGCGTCVASHERTLRKHGLSREAIQSAI
                     RIAATVHAVARVLEDATRQTGTTAQAA"
     gene            complement(10524..11060)
                     /locus_tag="VFB37_03360"
     CDS             complement(10524..11060)
                     /locus_tag="VFB37_03360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO21517.1"
                     /translation="MLAIGQKFPAFALTGVVSSDAQAFQPFTQDSFPGKWQVVFFWPK
                     DFTFVCPTEIAAFGKLDKEFKARDAQLLGVSIDSEYVHLAWRKEKEELRKLPFPMLSD
                     LKRELTSQLGILDPQAGVAQRATYIVDPQGIIRFVYVTDLSVGRNPDEVLRVLDALQT
                     DELCPCNWQQGEDTLKAA"
     gene            11176..12072
                     /locus_tag="VFB37_03365"
     CDS             11176..12072
                     /locus_tag="VFB37_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZO21518.1"
                     /translation="MPDIKLKDLRYLIAVADSRHFGRAAQSCFVSQPTLSAQLKKLED
                     SLGVQLIERQPGNVTLTDAGEQVVARARRILEASEELVTLARALRDPLAGRLRLALLP
                     TIGPYLLPRVSQPIRKALSRIELRLYEFQTAQMLEKLTAGDIDVGILALPVELEGLES
                     RELYAEPFTVALPEQHRLAKRSSIRVEDLEGETLLLLEDGHCLRDQALEVCSRIGLNE
                     KQDFRATSLETLRQMVATGAGITLLPELAARGAYGNARGVSIRPFTRPPPVRHVGAVW
                     RKSSARRIAIDALCDVVAEHAK"
     gene            12133..12573
                     /locus_tag="VFB37_03370"
     CDS             12133..12573
                     /locus_tag="VFB37_03370"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HZO21519.1"
                     /translation="MKLTGFVARVSLGALVVLAGAAQTASLAVQNAWIRATPGSDVAA
                     AYFTLHNSSAAPVTITGVYSPAASQAMIHETSVQGGQSRMRPHEHLLIPPGQTVKLEP
                     GGLHVMLTGLKRPLAVGQSVPLVLALAGGSTLQLTATVRPLTAE"
     gene            12570..13904
                     /locus_tag="VFB37_03375"
     CDS             12570..13904
                     /locus_tag="VFB37_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO21520.1"
                     /translation="MSPVSAGRQDSPAEHVTPYDRFGWSDSEVERLLATGKHEHELTA
                     YFGAAEYRALRRLAKRCKRSSPAKGAPPVFVVPGIMGSQLGLRRAKPLPQDVLWLDPI
                     DIGVGRLAALRLPPRAPIVPLGVVLYSYLRLKLHLRAGGFAPVFHDYDWRLRVEELGA
                     ALAQRIRATPGKRIMIVAHSMGGLVSRAALTQPGTDRVERVVLLGTPNLGSFAPLQAL
                     RGTYSVVRKIARLVSADTAEALAENIFNTFPSLYQMLPVSQRTAGTDLFDRAQWPASG
                     PQPDPALLRSARAIQSRMAPGDERMCPVVGIGQETITSVSRRANEFVYTITRHGDGTV
                     PAASASLPGARNHYVPVPHSELTRDPVVAAAVNDLLHRGTTKLFPSKWRSDSRAEARI
                     SDTQLRRMHTGKVDWAGLLPEERREFLQTLNEPPKLRLRVPGRRKSPRGKRP"
     gene            13901..14422
                     /locus_tag="VFB37_03380"
     CDS             13901..14422
                     /locus_tag="VFB37_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZO21521.1"
                     /translation="MSSGFEEITRAVPDMAQVVRIVIRLLIALLIGSLVGLQRELTHK
                     PAGLRTHMLVSLGTALFIVGALEAGMAHDDLSRVVQGLATGIGFLGGGAILKLTTERE
                     IHGLTTAAGIWMTAAASAAAALGQIAVALIGTVLALLVLTAFRKLEKELGHRAKKDSE
                     THPSGNPDPRDLM"
     gene            complement(14441..>14627)
                     /locus_tag="VFB37_03385"
     CDS             complement(14441..>14627)
                     /locus_tag="VFB37_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21522.1"
                     /translation="AAELECKSWGQVFLKYIAANPAVTCIIPATGKLTHLQDNLGGGR
                     GRLPDARQRELILQALF"
BASE COUNT         2598 a         4935 c         4674 g         2420 t
ORIGIN      
        1 agggcacgaa acacgagcag cgagccgaag tttggagcga gcgtacagcc gatcgaggcc
       61 gccgtgtagg ttgcgagccc cgcgagaatc acgggccggc gtcccaacgc atccgatagc
      121 ggcccctgca cgagcgacat cacagcaagc ggcagcatgt agacggtgag tgtctgctga
      181 acggcccacc gactgagatg gaaatgcgcc gcgatggtgt ggaaagacgg gaagaaagta
      241 tcgatcgaga aaggactcac catggacagc accgccagca gtgccgccag cttccactgc
      301 gtctctgatc ggtgatctgc gctcacccgg tctcccgcgc ctccggtgcg gtggctcgca
      361 gagcgcgcag ccactgcgtt ggagcctctt cacgctccag aacttccacc agcgccgaac
      421 cgatcacgac gccgtccaca tggccgcgca gccgctgcac ctgctcccgg gtccgaatac
      481 cgaagccggc gcacaccgga actggagcga gcgagcgcac ccgatcgaga taacccatga
      541 cttccggtgg cacagccaca ttcttccccg tcgtgccggt catcgtcacc gcgtacacga
      601 agccctggct actctcgcaa agctgctgca tccgctgcgc ccgggtgacg ggtgttacca
      661 tctgtaccat tgcgatgccg tgagcatcca gtgcctggcg cagctcagct ccctcatcgt
      721 aggggagatc cggcacgatg aatcccgaga cccgcgcccg taccgaggct tgcgcaagct
      781 catccagccc gtaggcgagc agtggattga gatagctcat caggagcaac ggggcgctgg
      841 ccgcaggcat ggcctccagc tctttcaaaa tccatcgcaa gctcacccct tgggagagag
      901 cgacctcgct cgagcgctga atcgtgacac cgtcggccat cgggtcgctg aatggaacac
      961 cgatttccac gacatcggcc ccgtcggcca cggcgtgcag atgctcgcga aatctctccc
     1021 ggctcggaaa accggctgtc aggaaagcaa ccagagccgg ttcaccgccg gcgctggcgg
     1081 cccggattgc ctgactgatg acatcacgcg ctttcattac ggctgctccg acgttgcttt
     1141 gttggccagc aacgtgctct gcaaggtgga catgtccttg tcaccgcgtc ccgagaggca
     1201 aacgaggatc cgtctgcccg gattgcgttg cgcccagcgc ctcgcgccca ccagcgcgtg
     1261 cgcgctttcc agagccggta agatcccttc gcactcgctg caggcgcgga ccgcctcgag
     1321 ggcttcttcg tcggaggcgg tctcataggc ggcgcggccc gttgcgagca gcaacgcatg
     1381 ctccggaccg acgccagggt aatccaggcc tgcggacacc gaatgggttt cctggatctg
     1441 accgttctcg tcctgcaaca gcatcgagaa gctgccctgc agcacaccgg ggctgccata
     1501 ggaaagagtg gccgcattgc ttcccaagct gcggccctgg ccgccggcct cgacgccgat
     1561 gatggcgacg gagcggtcag gcacgaacgc gtggaaggcg cctatcgagt ttgagcctcc
     1621 ccccacgcat gcgatcacta catcgggcag gcttccggtc tcttcgagca tctgcgctcg
     1681 cgcctcgcgg ccgatgaccg cctgcagctc ccgcacaagg taaggatagg gatgggggcc
     1741 gacggcggag ccgatcagat agtaggtgtc gagcggatcg ctcacccagt cgcgcagcgc
     1801 ctcgtcaatc gcggcgcgca gcgtacgatc gcccgtggtc accgcaacta cgattgcacc
     1861 cagcaggcgc attctgccga cattgggggc ttgcctctcc atgtcgaccg agcccatgta
     1921 caccgtgcac ggcaagccga gtcgcgcgca agcggccgca ctggcgacac catgctgccc
     1981 cgcgccggtt tcggcaacga tgcgcttcgc gcccagccgc ttcgcgagca gcgcctgtcc
     2041 gatggcgttg ttgatcttgt gggcccccgt atgcgcaagg tcttcgcgct tcagccacac
     2101 ctccgcgccc cagcgctcgg acaatcgcgc cgcaaaggtc agcgccgtgg gtcgtcccac
     2161 ccaagtccgc agctgatgct gcagttcgct ctggaaaagg cgcgcgtgca aatgctctgc
     2221 aataccgcct tccagacgct ccaatgcagg aatcagggtc tcgggcacgt agcgaccgcc
     2281 gaaggggcca taacgccccc gcgcgtcggg aaacttgccc gccatggagt ccgcgagcag
     2341 ccgctcggcg tctatcgctg gggtcatcac cgtgatttct cctctacatg cggcgagccg
     2401 gcctgatcgt gcgcgcgtac cgctgagacg aaacgcctga tctcggccgg atccttgatg
     2461 gcacgctctc gctcgacgcc gctggagacg tccacgccaa aagggcgaac cgccgtcatg
     2521 gccgtaacaa cgttcgcgga ggtcaaaccg cccgccagga cgagttcaac ttggcgcgct
     2581 agccggctcg caccgtccca gtcggcgcgc attcccaccc cgctgtcggc agcctcgaac
     2641 aacactctcc cccggggctg ccgaccctca tcctgccaga ggcggatgac cgggagtacc
     2701 tcgagctgtt gcgggatctg caaggtgcga agatcctcga ggtcggtctg cagcgcatcc
     2761 ggccggaagc gctccaggat ttcctcgagg ctgtcctgag tgggatgacg catcactgcg
     2821 atgcaggcaa tgcggccccg agcggggccg gcaaggccgg ctgccgcctg cggcgtcaat
     2881 tgccgtggcg aaggcgcgaa tacgaatcca atcgcatcga ccccggtctc gagcgcggta
     2941 tccaccgccg ctggggtcgt catcccgcag atcttgatcc aggtcatgag ctgcccatgg
     3001 cggctctgcc cgcactgagc aatgcttccg ccagaacgtg cggatcggtc tgcatcaagg
     3061 cgctgccaac caacatgtaa cggtaaccgg cggcggccac gcggcgggca tcttccgggc
     3121 tgcgcacacc gctctcggcc acccgcggca cgcccgccgg cagcagatcg acgagctcga
     3181 ggaggcgctc cggcacgatc tgcaggctgg atagatcgcg gcaattaagc ccggcgagca
     3241 ccggcgccgg cgtgccggca gcgccgctgt gcgcagcatg agattggagg atgtcatgca
     3301 tgatctcgat gtcgtgctcg tcaaaagcct ccagcagcac gaacattccg agctgtcgtg
     3361 cgcagtccat cagggaatgg atctgcgcga cgggaagcat cctcaggatg accagcactc
     3421 cgcccgcccc ggcgacgcgt gcttcgatca cctgataggg atcgaccaga aagtccttac
     3481 gcatggccgg aacctgcagc gggcgcagcg cacgcgccgc gagctcgagg tgcgccatgg
     3541 agccgtcgaa gcgactcggc tctgtcagga tggagacggc ggctgcgccg gcccctgcgt
     3601 acacctcgac acgcgcccca acatcctcac tcaccggcct gagctgtcct tcggcaggcg
     3661 atcgcagctt gagctccgcg atcagatcga atccttccgg gctcgcgcgc aaggctggag
     3721 ctggacctga agccaacgcc cgctcgagca gcgccgcctc cgtcacacgc gcccgggccc
     3781 gagccgcacg ctcacggctg ctcgcggcca tctgcttcag gaaccctgag tcgccgctca
     3841 taccggcagc gcagtcggcc gcgatgcgcc gcgtgctgcg gaaaaggccg ccagactctc
     3901 gagcacgcgg cgcgatgcgc cgctgtcaat cgcttccacg gcgcgctcaa ccgcttcgtg
     3961 cggcgtgcga gcttgtcccg tgacctcgag agcgagtgca gcgccgagca acaggcaatc
     4021 cctgtgcggt cctcgatcct ctcccatgag aaccgcccgc aaggcgcgag cgttgtgctg
     4081 cgcatcgccg ccggcaagac cttcggccgt acacctgctc aggcgatagt cctgcggctc
     4141 acgcacctcg gccacgatcc ggcccggcct gacatcgaac agctcgaagg gcccagcggg
     4201 ggtcggctcg tcccagccgt gggcgccgtg cacgacaaag ctgcgctcaa cctccatgcc
     4261 ggcaagcgtt tgtgcgatga gctgcgctac ctcgcggctg taggcgccga tcaggtggaa
     4321 gggaggttcg cccgggttgg tcagcggccc gaggatgttg aatatggtcc gcaccccgag
     4381 ggccgccctc acgggcgcca cggccttcat tgccggatgg taatgagggg cgaacaggaa
     4441 ggtgaaattg cacgcggcca gacactcgcc ggctgcctgc tcgtcgagcg gaatctcgag
     4501 tccgagtgct tccagcacat ccgcactgcc ggcacggctc gatactgagc ggttgccatg
     4561 tttcaggacg ggcagaccac atgccgccgc cagcaacgcc gtgccggtgg agatgttgag
     4621 acttccggaa gcatcccccc cggtgccgac cacatcgatg gcacgcaagc ccgccggaac
     4681 ctgaggtcgc cgcgcgagcc gccgcatgcc acgcgcgaat ccacgaacct catcggcgac
     4741 gacgcccttg ctactcaggg ccgcgaggat cgcaccggcc atgggtggcg gacactgggc
     4801 gctggtcaga cacgtgagca gctcctcggc ctgcgactcg gccaggtcgc gccgatccag
     4861 caggatttcc agcatctcac gcatggcagc gggcggcgaa gtccgggcgc aggccgccag
     4921 tcaggcgcag gaagttctcc agcaagcgcg ggccttgcgg cgtcaggatg ctttccggat
     4981 gaaactgcac accctccagg acgagctcgc gatggcgcac gcccatgatc tccccctcca
     5041 gcgtgcgtgc gctgatttcc agctcgcccg gcagggactc aagctcggcg atcagcgagt
     5101 gataacgccc cacctcgcac gcctggggca atcctgcaaa cacggtgcgc tcatcgtgat
     5161 gaacgatcga agtcttgccg tgcatgagcc gcccggcgcg caccactttc ccgccgaaca
     5221 cctccaccat ggattgatgt cccaggcaga cgcccagcac cggaatacgg cccgcaaagg
     5281 cacggatcat atccatggac accccggcat ccgccggcgt gcctggaccg ggcgagatgc
     5341 acagatggct cggagacaga tcacgcgcct gctggggcgt gatcgcatca ttgcgatgca
     5401 cttcgacatc tgcgcccagc accagaaatg cctgcacgag gttgtaggta aaggaatcgt
     5461 agttgtcgat gagcagaacc tgcggtttca cagcccaccc ccgcccgcgc tggcatcgca
     5521 cgtcatttcc agcgcgcgcc ccatcgcggc gctcttggcg agcacttcct cgtattccgt
     5581 ttcggggacg ctgtcggcca cgatgcccgc gccagcctga aagctgtacc gatccgcgtt
     5641 gaagaccaga gtacggatgg taatcgcgtg atccatgtcg ccgcgcgccc cgaagtatcc
     5701 aaccgtgccg ccgtacaagc cccgattggt cggctccagc tcagcgatga tctgcatcgc
     5761 gcgtaccttg ggtgcaccga cgagtgtgcc tgcggggaag gccgcggcga acaggtcgaa
     5821 tgcatcgcgg ctgtcttcca gcaggccgtg caccccgctc acgatgtgca tgacgtggct
     5881 gtagcgctcg atggcccgat agggatcgac ccgaacgctg cctgggcgcg ccacgcgtcc
     5941 caggtcatta cgcgccaggt cgaccagcat gacatgctcg gcattctcct tcggatcagc
     6001 gcgcagttcc gcctctcgcg ccaggtcggt cgacaactcg tccgagcgcg gccgtgtgcc
     6061 ggctatcggc cgcagctggg cgtgaccgtc cttgagtttg acgagcgcct cgggcgagga
     6121 tcccacgacc gtcacatcgc ccagagcgca gtaatacatg tagggcgagg gattgatcag
     6181 cctgagagcc cgataggcct gaaagggctc gatctcgtgc cggccctcga atcgggccga
     6241 gagcaccagc tgatacacat cgccggctgc aatgtattcc tgcacgcgcc tgacaccttc
     6301 catgtagtcg gctcgcgcat aactcgcagt cggctgcgag tagcgtccca cacgccggcc
     6361 tgcgcccggc aaggccccgc gcagcgcgct gatgatctcg gtgcgcaggg agcgacgctc
     6421 ctgctcggag cccgcatgca gcaacgctat tcctcgcgta aggtgatcga acaccagcag
     6481 cgagcagggc gcgacatagt gcagcagcgg cactccgctg agcgttggag cggatactcg
     6541 ctcgaaagac cgcaccacat cgtacgccgc gtagccgacg aggccgcccg ccaacggcac
     6601 gcccgcgatg tcaggccggg gctggggagc tctgcgcaat gcctcgcgca gcgccgcaag
     6661 gagcgctgcg ccgtccgccg gagccggccg gcgctcaccg ccgatgaaca gggcgtcccg
     6721 atcgagccgg acctccagcc cgtcgccgaa gccgatgaac gagtaccggc ccaggcgctc
     6781 gccgccctcc acgctctcga gcaggaaccg cggcgcgaac gcggccagct tgatgaaagc
     6841 cgataccggc gtatccagat cacctgcaat gtcgaacggc ggtttcaaca tcactccaaa
     6901 agcgaaaaac ccatcccggc gtgaccggag atgggttctg gtgcttggga acttgagact
     6961 gtcgagaaac tagcagcgac cgggcccact ctccgtcagg aagtgcgcca ccaccatacg
     7021 ttgaagcaga gtgcctgcgg ctgccgtgac atttgcgacc gagtatcgcc gggccgcggc
     7081 tcgggcgtca agtgcgtaaa ctgcattaaa ataccctcgt ccaacgtggc cacgcggagc
     7141 gactcgtgag tttcaaaaag ctgctcgaag gctggcgaga gagcgcggcg gcgccgcgca
     7201 cggcggcgga gtatgcggtg cggctgccgg tcgacgatgc cgcgcggctg cgagctctgg
     7261 ctgacatgtt cccggggcgc tccacggagc agctcatcac ggacctgctc ggcaccgccc
     7321 tccaggagat tgccgccgcg atgccctacg tggccggaca caaggttatt tcgacagacg
     7381 accaggggga tcctgtctac gaggatgtcg gccccactcc gcgtttcatg gagctcgctc
     7441 gacggcatcg ccgcaagctc gaggcggagc tgaagaaagc cgcaggctga ggagaacgtc
     7501 atgccctcct tcgatgtggt ctcggagctc aacattcacg agctgagcaa cgcgctcgac
     7561 caggccaacc gcgagctggc gcagcgcttc gatttcaagg actcgggcgc cgtcttcgag
     7621 ctcaaggagt tcaccgtcac gctgcgagcg caggtggact ttcagctcaa gcagatgctg
     7681 gagatcctga agctgcggct caccaagcgc ggcatcgatc tggcgtgcct ggaggtgaag
     7741 gagccgcaga cgacactggc cagcgcacaa caggaagttg tgctcaaaca cggcatcgat
     7801 caggaaaccg gcaagaagat catgcggcgg atgaaggatt cgaagctcaa ggtgcaggcc
     7861 gccctgcagg cggacaaggt gcgcgtcacc ggcaagcagc gcgatgagct gcaacaggcg
     7921 attcagcttc tgcgcgccga gaagctcggc gtgccgctgc agttcaacaa tttccgcgat
     7981 taatgcagcg cggcgagcgc ctgacacagc ccttcggtcg catcgagaag gctgggcccg
     8041 agtcgcgtca agcgccgatc ctcgaaggcg atcagatggc cggtacgcac cgcgcgcagc
     8101 gtcgcgaagc gtttccagtc cgcgagccac tgcgccgcat ttccgggcgg cgccgctgcg
     8161 acgatgacat ccggatcccg ggcaatcacg gcctcgacgt cgattgccgg ggcaagctga
     8221 cgcagatcgc cgaacgcatt gcgcgccccg cacagctgca gggcatcgct catgagctgg
     8281 gtaccaccga cggtgtagat cgggtgatcc cagatctgca gcagcaccgt cggatgcggg
     8341 cccgctccat aggtgcgccg gagtctggcc acgcgagctt ccaggtcgcg ggcggccggc
     8401 tccgcaatgg cgctcgttcc cgcgagctga cccaagcggc gcagcgagcc gggaagatcg
     8461 gccagagagt tgacctgctg gctgtactcg gggatcccca ttctgcggat ctgttcgatc
     8521 tgcgcaggat tgccgccgcc cggccagatc acggcgacct ggggtcgcag ggccacgagg
     8581 cgttccatgt cgatcgcaac cacatcgccg atgcgtggca cgcggcgcgc cgccgccggc
     8641 tcgtcggaat attcgactgt tgcaatcacc tgggcgcccg cgccggctgc gaataacatc
     8701 tccgtcgcgc ccggtgtcag gctgacaata cgttgcggat gatgtggcag cgacagcgtc
     8761 tgcccggcgt catccgtcac ggtcacgggc ggatcggccg ccggtagcgg cgctgtcgcc
     8821 agagcccaca ccagcacggc cgccagcgcc cgcctggcgg ccggcggcgg cgagttcact
     8881 tgatcgagcc gggcttgcgc atcatcttgc gaatctccga gaggtgctcc tcctcggtcg
     8941 caatctgcag gcgtgcatat tcctccagca tgatgctctt gcccgccacc agctccagca
     9001 gcgcgcgata ttcggccagc ccttcctcct cgtgtgcgtg cgcctcgccc aggatctcat
     9061 ccacgttgtg tttgtgcgtc tccagcagcg acccgatccg caacgaagga tgtccgccca
     9121 ggttcgtgat gtgctcgccc gccttgaccg cgtgtgcctg ggactcatcc gcctgagcac
     9181 gcagccagcc gacgatggga atgcggttat gcccgaaaat catgaagctg tagtgcagat
     9241 aacggaccag acccgccagt tcgagctcca ggatccggtt caggatcgtg acgatcttct
     9301 cgttggtatc cgtggcgctg gtcgatgagc tcatgacggc actcctctcg cggtggagcg
     9361 cgattgtctg ccgccggctc catgcaggtc cagcagttcg cgaacccccg ggcgcctcga
     9421 tccgctagcg gattccgatg gtcgccgccg cccagcgcag gatgggatcc aggcgcggcg
     9481 agcacagcga cagtccctgc tcgaaactca cccagcgcca ctcgttgtgc tccggccgac
     9541 ccagttgcgg gttcaccggc aaggtcacgg cctcggtggc cgtctctgca atgtagtaac
     9601 gcgcgacctt cttgtgactg tagggcgcgg tctccacgta ggcctcgccc caggtgaact
     9661 gcaggtccgc gagcagagtc tcctcctgga cctcgcgcag cgcaccggcg agcggctgct
     9721 cgcccgcctc caccaaaccc ttggggaagt cccagttacg ataggcgcgc agcatcagaa
     9781 agcgccacgc ctcgcgcgca tttctgacga cgatcactcc cgcggacaga cgggcggaca
     9841 tgtggcccct ccctgatccc cgcagcggat tctgcaacgg cgcgccaggc gccgtaaatc
     9901 ggccctcagg cggcctgagc cgtcgtgccg gtctggcgag tcgcatcctc cagcacgcgt
     9961 gccacggcat ggacggtcgc cgcgatccga atcgcactct ggatcgcctc gcggctcaac
    10021 ccgtgcttgc gcagcgtgcg ctcgtgcgag gccacacagg tgccacaccc gttgatggcc
    10081 gaaaccgcca gtgacagcag ctcgaagtcc tgcttgtcga tccccggatt gccgatgacg
    10141 ttcatgcgca gccgcgcggg cagctgtgca tattcaggat cctcgacgag gtgcaggaag
    10201 cggtagtaga cgttgttcat gcccatgatg gccgccgcag cccgcgctgc attgaggtgg
    10261 gcagcgtcga ggtgggagct gctcagagct tcgatgtcgg tcgagaaagt cagattgcgc
    10321 gaggcaatcg ccgcggcgac tgcgaccgac cagatctgct gctcgctcag tccaggggcc
    10381 ccggtgctcg ccaggacgct tccgagattg agtctcagat cgcgcgcgta ctcggggatg
    10441 gcgtcgcgaa tggtatcgag cgtggtcatg atgatcctct gcggggcgcg cagcgccccg
    10501 ctcgttgatg cgcgtgaaag ctgtcaggca gccttgagtg tgtcctcgcc ctgctgccag
    10561 ttgcaggggc acagctcatc tgtctgcaga gcgtccagaa cccgcagcac ttcgtccgga
    10621 tttcgcccga cgctgagatc ggtgacgtaa acgaagcgaa tgatgccctg gggatccacg
    10681 atgtacgtgg cccgctgtgc gacacccgct tgcggatcga gaatacccag ctggctcgtc
    10741 agctcgcgct tgaggtcgga gagcatcggg aacggaagct tgcgcagctc ctccttctcc
    10801 ttgcgccagg ccagatgcac gtactcactg tcgatgctca ctccgagcaa ctgcgcatcg
    10861 cgggctttga attccttgtc gagcttgccg aacgcggcaa tctcggtcgg gcacacgaag
    10921 gtgaagtcct tcggccagaa gaagacgacc tgccacttgc ccgggaagct gtcctgggtg
    10981 aagggctgaa aggcctgggc atcgcttgaa acgacgccgg tgagcgcgaa cgcggggaat
    11041 ttctgtccaa tagctagcat tccatgactc ctcgaggatt gactcctgcg gtaggcattg
    11101 atcatggggg cgggcgcttc atagttccaa tcgattgata gtataatctc gatagtctct
    11161 ataaatggtt gtgccgtgcc cgacatcaag ctgaaggatc tgcgctatct gatcgccgtc
    11221 gccgacagcc gccacttcgg gcgcgctgcg cagagctgct tcgtcagtca gcccaccctg
    11281 tcggctcagc tcaagaagct cgaggactcc ctcggcgtgc agctcatcga gcgccagccc
    11341 ggcaacgtga cgctcacgga cgctggtgag caggtcgtcg cgcgcgcacg gcgcattctg
    11401 gaagccagcg aggagctggt gaccctcgca cgggcacttc gcgatccgct tgccggacgg
    11461 ctgcgcctcg cactgctgcc aacgatcggt ccgtatcttc tgcctcgtgt ttcgcagccg
    11521 attcgcaagg cgctgtcgcg aatcgagctg cgcctgtacg agtttcagac ggcgcagatg
    11581 ctggagaagc tgaccgccgg ggacatcgac gttgggattc tggcgctgcc cgtggaactg
    11641 gagggcctcg aatcacggga gctgtacgcg gaacctttca ccgtggccct gcccgagcag
    11701 caccgcctcg ccaagcgcag cagcatccgg gtggaggacc tcgaagggga gacgctgctg
    11761 ctgctcgagg acggccattg tctgcgtgac caggctctcg aggtgtgcag ccggatcggt
    11821 ctgaatgaga agcaggattt tcgcgcgacc agcctggaaa cgctccggca gatggtggcc
    11881 acgggagcgg gcatcacctt gctgccggag ctggctgccc gcggcgccta tggcaatgcc
    11941 cggggagttt cgatccggcc cttcacccgg cctccgccgg tcagacacgt cggcgccgtt
    12001 tggcgcaagt cgagcgcaag gcgaatcgcc atcgatgcgt tgtgcgatgt cgtggcagag
    12061 cacgcgaagt agggtctggc ataatccccg gccaggagcc gaaggcggct tcgcgcatcg
    12121 agagagcccg ccgtgaagct gacaggtttc gttgctcgtg tgtccctggg cgcgctggtc
    12181 gtgctcgcag gggcagcgca gacagcctcc cttgcggtgc aaaatgcctg gatacgcgcg
    12241 acaccgggct cggacgttgc cgccgcctac ttcacgctcc ataacagcag cgcagcccct
    12301 gtcaccatca cgggtgtcta ctcgcccgcg gcttcacagg cgatgatcca tgagacctcg
    12361 gtgcagggtg ggcagtcgcg catgcgcccg catgagcatc tgctgattcc acccggacag
    12421 accgtcaagc tcgagcccgg cggcctgcac gtgatgttga cgggtctgaa acggccgctc
    12481 gcggtgggtc agagtgtgcc gctcgtgctg gccctcgccg gaggcagcac gctgcagctc
    12541 acggcaaccg tccggcccct gactgccgaa tgagcccggt cagcgcgggc cggcaggatt
    12601 ctccggccga acacgtgacc ccgtacgatc gcttcggctg gagcgatagc gaggtcgagc
    12661 gcctgctcgc gaccggcaaa cacgagcacg agctgactgc ctactttggc gcggccgaat
    12721 accgcgcgct gaggaggctg gcgaagcgct gtaagcgctc ctcgcccgct aagggtgcgc
    12781 cgccggtttt cgtcgtcccc ggaatcatgg ggtcacagct ggggctgcgg cgggccaaac
    12841 ccttgccgca ggatgttcta tggctcgatc cgatcgacat cggcgtcggc cggctcgccg
    12901 cgctgcgtct gcccccgcgt gcaccgatcg tgccgctcgg ggtcgttctt tactcctatc
    12961 tgcggctcaa gttgcacctg cgcgccggcg gctttgctcc cgtgttccac gactacgact
    13021 ggaggctccg cgtcgaggag ctcggggctg ccttggcgca gcgtatccgg gccactccgg
    13081 gcaagcgcat catgatcgtc gcccacagca tgggtggact ggtcagccgg gccgcgctga
    13141 cacaacccgg tacagaccgg gtggagcgcg tcgtgttgct cggcactccc aacctgggct
    13201 cgttcgcgcc tctgcaggcc ctgcgaggga cctactcggt cgtgcgcaag atcgcccggc
    13261 tggtgagcgc cgacaccgcc gaagccctgg ccgaaaacat attcaacacc ttccccagcc
    13321 tctaccagat gcttccggtg agccagcgca ccgcgggcac ggatctgttc gatcgggcgc
    13381 aatggccggc ttccgggccg caacccgatc cggcgttgct gcgcagcgca cgcgcgattc
    13441 aaagccggat ggcccctggc gatgagcgga tgtgtccggt cgtaggcatt ggtcaggaga
    13501 cgatcacctc tgtcagccgg cgtgccaatg agttcgtcta taccatcacc cggcacggag
    13561 acggcacggt tcccgcagcg agtgcgagtt tgcccggcgc gcgcaaccac tatgtcccgg
    13621 tgccccacag cgagctcacc agagatccgg tcgtagcggc ggccgtcaac gaccttctgc
    13681 atcgtggcac cacgaagctc tttccctcca agtggcgcag tgacagtcgc gccgaggcgc
    13741 gcatcagcga cacgcagttg cggcggatgc atactgggaa ggtggactgg gcgggcctgc
    13801 tgccggagga acggcgcgag tttttgcaga ccctcaatga gccgccgaag ctgcggctgc
    13861 gagtgcccgg ccgaagaaag agcccacggg ggaagcggcc atgagcagcg gcttcgagga
    13921 gatcacgcgc gccgtgccgg atatggcgca ggttgtgcgc atcgtcatcc ggctgctgat
    13981 tgcgctcctg atcggctcac tggtcggcct gcaacgcgag ctgactcaca agccggccgg
    14041 actgcgcacc cacatgctgg tcagcctcgg cacagcgctg ttcatcgtgg gcgcgctgga
    14101 agcgggcatg gcgcacgacg acctgtcgcg cgtggtgcaa ggattggcca cgggcatcgg
    14161 cttcctgggc ggcggggcca tcctcaaact gacaacggag cgcgagattc acggtctcac
    14221 gactgccgcg ggaatctgga tgaccgcagc ggccagcgcc gcagccgcgc tcggtcagat
    14281 cgcggtcgcg ctgatcggca ccgtgctggc cctgctcgtg ttgacggcct tccgcaagct
    14341 ggagaaggag ctcggtcacc gcgcgaagaa ggactccgaa acgcatccgt ccggcaatcc
    14401 ggatccgcgt gacctcatgt gaagcggccc gcgcgccgat tcagaagagg gcctgaagaa
    14461 tcaactcccg ctggcgcgca tccggaagcc tcccgcgtcc gccgccgaga ttatcctgaa
    14521 gatgggtcag cttcccggtc gcgggaatga tgcaggtcac ggccggattg gcagcgatgt
    14581 acttgaggaa cacctggccc cagctcttgc attcgagctc ggccgcc
//
LOCUS       DASRHN010000089         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2008807, whole genome shotgun sequence.
ACCESSION   DASRHN010000089 DASRHN010000000
VERSION     DASRHN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008807"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFB37_03390"
     CDS             complement(<1..341)
                     /locus_tag="VFB37_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO21523.1"
                     /translation="MIARHRCADGYTFSRIIKGGWHLAGDHGVIDPQQAIQDMRHFVE
                     AGITTFDCADIYSGVESLIGAFRRAHPQAARRLQIHTKFVPDLPDLARLSRRDVEGII
                     DRSLQRLGVEQL"
     gene            complement(338..727)
                     /locus_tag="VFB37_03395"
     CDS             complement(338..727)
                     /locus_tag="VFB37_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HZO21524.1"
                     /translation="MSERGSSLQPIERGPGEAGFELYDLRVEVVGPSDQPFYCGARLG
                     DHFELHGEMLQLPPGQGFSIYSLAALLPLLPAKQRVTDPHDWMSSDAEVACPDPNCPS
                     RFRITRTGRRYFRRADTTATPLVAEPL"
     gene            complement(724..1425)
                     /locus_tag="VFB37_03400"
     CDS             complement(724..1425)
                     /locus_tag="VFB37_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO21525.1"
                     /translation="MRAQPKLSLAPIARETVQERVYSVLRERLMRGAFEPGQKLTIVA
                     LAQALGTSAMPVREALNRLSAERAIESMPNRSVRVPSLSRDALRDLMDARFAIEGLAV
                     TRAAANMDRRTLERLRKLIVAQSRTDAQHVSEASSERNREFHFTIYARSGSSVLLPII
                     ESLWLQFGPYLRVASERFDGRDGLGTNFHEALVAALQRGDGRSARRALESDIGRSFRL
                     VMTDEAFWHRQEREK"
     gene            2241..2555
                     /locus_tag="VFB37_03405"
     CDS             2241..2555
                     /locus_tag="VFB37_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21526.1"
                     /translation="MWMRIFGIIGAGFMVLYGALGVLRNDLHVSLSKSSSSGVHLHGF
                     LAWLCFAGMMMMSIGLVRLLAPDAGASEFDFDARRHRFGPLFLSGLVLFGVAQLIADL
                     RS"
BASE COUNT          482 a          837 c          794 g          493 t
ORIGIN      
        1 agctgctcga cgccaagacg ctgcagcgaa cggtcaatga tgccctcgac atcgcgacgg
       61 ctcaggcgcg ccagatccgg cagatcgggc acgaatttcg tgtgaatctg aaggcggcgt
      121 gctgcttgcg ggtgtgcccg acggaacgcg ccgatcaggc tctcgacccc cgagtagatg
      181 tcagcgcagt cgaaggtcgt gatcccggcc tccacgaagt ggcgcatgtc ctggatggcc
      241 tgttgcggat cgatgactcc gtggtcgccg gccaggtgcc atcctccctt gatgatgcgg
      301 gagaacgtgt agccatccgc gcagcgatga cgtgcaatca cagcggttca gccaccaagg
      361 gagttgccgt ggtgtcggcg cgccggaaat accgccgtcc cgtccgggtt atacggaagc
      421 ggctcgggca gttcggatcc gggcaggcaa cctcggcatc cgaggacatc caatcgtgcg
      481 gatcggtgac ccgctgcttg gccggcagca gcggcagtaa cgccgccagg gaatagatcg
      541 aaaacccctg cccgggcggc agctgcagca tctccccgtg aagctcgaag tggtctccga
      601 gccgcgcgcc gcaatagaag ggctgatcgc tgggtcccac cacctcgacc cgcaggtcat
      661 agagttcgaa ccctgcctcc ccgggtccac gctcgatcgg ctggagactc gatccgcgct
      721 cactcatttt tccctctctt gccgatgcca gaaggcctca tcggtcatga ccagccggaa
      781 agagcgtcca atatccgatt ccagcgcccg gcgtgcactc cgcccgtcgc cgcgctgcag
      841 cgccgcgacc agcgcttcgt ggaaattcgt acccagacca tctcgcccgt cgaagcgctc
      901 cgaggccacg cgcagataag gtccgaactg cagccacagg ctctcgatga tgggcagcag
      961 cacgctcgag ccggagcgcg cgtatatggt gaaatggaac tcgcgattac gctccgagct
     1021 ggcttccgag acgtgctgtg catcggtgcg gctctgcgcc acgatcagct tgcgcaaacg
     1081 ctcgagcgtc cgccggtcca tgttcgccgc cgcgcgcgtc acggcaaggc cctcgatggc
     1141 aaagcgagca tccatgagat cgcgcagcgc atccctggac agcgacggca ctcgcacact
     1201 gcggttcggc atcgattcga tcgcccgctc ggcgctcagc cgattcagcg cctcgcgcac
     1261 cggcatcgca ctcgtgccga gcgcctgggc cagcgccacg atcgtcaatt tctgtccggg
     1321 ctcgaaggct ccgcgcatca gtcgctcacg caataccgag tacacgcgct cctgcacggt
     1381 ctcgcgcgcg atgggtgcca gtgacagctt cggctgcgcc cgcatgggca ctccttgatc
     1441 tttgatcaaa gatttataac caaaaactca ccgactggca accggccgca agtcagcgtc
     1501 acgggaggcg aatcgaacga gcgtgcgagc tgcgccgcaa catttgagga ctgactggaa
     1561 gtggccggcc aggctcagag tatgctgcga atgatcggcc ttactcaggg taacgagggt
     1621 tcccgccgcc tgtttgtttc agcggcaaag cggcgagtct gagtaggagc atcgatgggg
     1681 tggccgttga tggccacgat ctcctcccca gccttacagc cgactcgctc cgctggactt
     1741 ccggggccac cagcaggacc ttgagactct tgttcgagaa tgactgccgt gaggaaggcc
     1801 gaccgtcagc gatgaggaca gcccggccaa cagcatggcc ggcgcttgcc attcttcagg
     1861 cttgaacagc cccactggat ccgacggctc ctggtctcgg ctttcttggc ggacatgatc
     1921 caacagatcc attcgttgcg cgccagcggc gtgatgtcct cccacgctgc cagcaccttc
     1981 gaatcagaca ggagcgcggc tcgtaggtcc gcggggacgt tgtgcacaac gcctgtcgca
     2041 attttctttc tcgccagcat tgcttgggaa agctaaaaag gacggccgac agcggtactt
     2101 catcacatta gaacatagct cacgaagcta tcgttcgtgg ccaccggtaa gcttaagcat
     2161 atgaaccgcg ataacgtctc ggaccacgtc ctacccctga gaggaatgcc atgatcgggg
     2221 ccgaccacag ggtcccctca atgtggatgc gcattttcgg gatcatcggg gcgggcttca
     2281 tggtgctgta cggcgctctc ggcgtgctgc gcaacgactt gcacgtttcc ttatcgaaat
     2341 ccagcagctc cggcgtgcac cttcacgggt tcctcgcgtg gctctgcttt gcgggaatga
     2401 tgatgatgtc gatcggactg gttcgcttgc tcgcccccga cgcaggtgcg agcgaatttg
     2461 attttgacgc gcgtcggcat cggtttgggc ccctgtttct cagtgggctg gtgcttttcg
     2521 gagttgccca gctcatcgct gacctgcggt catgatgcca atcgcgcggg cggttagagg
     2581 gcagcccgta tcgccaagcc aatctg
//
LOCUS       DASRHN010000090         6499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1679028, whole genome shotgun sequence.
ACCESSION   DASRHN010000090 DASRHN010000000
VERSION     DASRHN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679028"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2539)
                     /gene="polA"
                     /locus_tag="VFB37_03410"
     CDS             complement(<1..2539)
                     /gene="polA"
                     /locus_tag="VFB37_03410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZO21527.1"
                     /translation="MTQSPDLVLVDGSSYVYRAFHALPPLTNSRGEPTGAVYGVLNML
                     LKFLKDYQPQRIAVVFDAAGRTFRDDLFAEYKAHRTPMPNDLRPQIEPLLSILRAQGV
                     PILRIEGVEADDVIGTLACRASRAGLRVLISTSDKDMAQLVDGSITLINTMSNTALDR
                     EGVKAKFDVYPEQIIDYLALIGDSSDNIPGIDKVGPKTAARLLEQYKALDSVIAHADQ
                     ISGRVGENIRAGLEVLELSRRLATIRTDLDLPLSHEQLMPTAPDTARLRELYTRYELR
                     SFLKQLEGTAPETAAEPASSAVPAGSGEQSPLAQVERHYECVTTWAELERWIGMLGAA
                     ELFAFDTATSTLDYMKAEIVGVSFALQRGVAAYVPLRHDYAGAPEQLDRDRVLAALKP
                     ILEDPARGKIGHHLKYDAHVLANYGVELAGMRFDTMLESYVLNSVATRHDVDSTALCY
                     LGMRTLTYEDVAGKGAKQLPFNQVPVDSAFRYAAEEADIALCLHHTLWPQLEAEPKLR
                     QLYENVERPLMPVLLRMERRGVLVDRERLRAQTREINHQLQELIEQARREAGAEFNID
                     SPRQLQQILFEKLQLPVTRRTPTGQPSTAEDVLEELASSYALPRIVLDYRVLAKLKST
                     YTDKLPELVDPRTGRIHTSYHQAVAATGRLSSADPNLQNIPIRRAEGRRIRQAFVAPP
                     GHVLMAADYSQIELRIMAHLSGDEGLLAAFAEDRDVHQATAAEVFGVPLENVSADQRR
                     LAKTINFGLIYGMSPFGLARQLGIDRGSAQKYVERYFARYPGVKRFMDETRLKARERG
                     YVETVFGRRLYLPEIKSGNSQLRQYAERSAINAPMQGTAADLIKRAMI"
     gene            2664..4097
                     /locus_tag="VFB37_03415"
     CDS             2664..4097
                     /locus_tag="VFB37_03415"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZO21528.1"
                     /translation="MQQAAADSLPAVAANRFGLGARPGELGRIGADPREWLRGQLAQA
                     PPLLIDANLQSSAETLAKALEARRELRQAKRSAEAERTGNGEAQADALAALLKVGQIY
                     RPVYMEEARARFREGVATDRPFIERLTYFWTNHFAVSVDKNIVLGLAGSLEREAVRPN
                     VLGHFADLLLAVEQHPAMLLYLDNHASVGPHSQAARAAERRNPGREIGINENLAREIM
                     ELHTLGVGGGYTQRDVTTFAEVLTGWSIGGSHGGLSGGEPGRFMFRPQLHEPGAKVVL
                     GRRYPEDGLGQGVAVLRHLAAHRSTAHFIATKLARHFIADDPPTGAVQRIAAAFTGSG
                     GHLPTVYRALIDSPEAWVAPLSKFKTPNDYILSTFRGLELPVDAGRAPLAPFEVMGQR
                     TYSPGSPAGWPDRSADWDGASALIKRLEWADAIGQRLGARRDAVALASQLLGGTLEEG
                     TRTAIARAASAAQALTLLLAAPEFMRR"
     gene            4131..5354
                     /locus_tag="VFB37_03420"
     CDS             4131..5354
                     /locus_tag="VFB37_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZO21529.1"
                     /translation="MASLKRREFLQAGALALGGSLMTSRLAFAGIGDGGDSRVVFVIM
                     RGALDGLAAVPPYGDSDYAALRRELTVGVPGAADGALPLDGFFGLHPHLGFLRDTYSA
                     GELIVFHAVASPYRERSHFDGQDVLENGSVHPHALQTGWLNRALASLQAGHAGPKELG
                     VVLGQNVPLLMRGPASVTSWSPSKLATLDDDTLQRVTDLYAADPLLSKRLADALAADA
                     IAAGSDSTMQGTAVAAKPARYAEIVHAAAGFLRRDDGPRVAVFDTTGWDTHANEGGAE
                     GSLAARLTALDDGLRMLKEGLGPVWSHTAVLLATEFGRTVATNGTRGTDHGTATAAFL
                     VGGAVKGGRVIADWPGLSARALYEGRDLKPTLDLRSVLKGVLAEHMRVSNGALEDWVF
                     PESTKATPIQGLMRA"
     gene            complement(5702..6121)
                     /locus_tag="VFB37_03425"
     CDS             complement(5702..6121)
                     /locus_tag="VFB37_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21530.1"
                     /translation="MAENLNASTTASAEANSSLLTYTHVIYALHTLSVVMGLATFWTI
                     AGSFIFGIPSIIAVIMNYARQRDARGSYLDSHFRWQIRTFWFALLWLVVIWAVSLPLM
                     LILVGLVTLWVGHVILGIWVIYRIARGWLALRDHRPV"
     gene            complement(6129..>6499)
                     /locus_tag="VFB37_03430"
     CDS             complement(6129..>6499)
                     /locus_tag="VFB37_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HZO21531.1"
                     /translation="HIGIRVAEVGPDYVRATMPVNPQTHQPMGVLHGGASVALAETVG
                     SLAASLCVDPQKHVCLGQEINANHIRPVSSGIVTATARPYHLGNRSQVWHIEIRDEQD
                     RLVCVSRLTMAVVDRPKNMS"
BASE COUNT         1096 a         2050 c         2187 g         1166 t
ORIGIN      
        1 agatcatggc gcgcttgatg agatccgctg cagtgccctg catcggcgcg ttgatcgcgc
       61 tgcgctccgc gtactgacgc agctgactgt tgcctgattt gatctcgggc agatacaaac
      121 gccgtccaaa gacagtctcg acgtatcccc gctcgcgcgc cttcaggcgc gtctcgtcca
      181 tgaaccgctt gacccccggg taacgcgcaa agtagcgctc cacgtatttc tgggcagagc
      241 cgcgatcgat accgagctgg cgcgccagac cgaatggcga catgccgtag atgagaccga
      301 agttgatcgt tttcgccagc cggcgctggt ccgccgagac attctcgagc ggcacgccga
      361 aaacttcggc ggcggtggcc tgatgcacgt cgcgatcctc ggcaaaggcc gcgagcagtc
      421 cctcgtctcc cgacaggtga gccatgattc gcagctcgat ctgcgagtag tcggcggcca
      481 tgagcacatg gccgggcggg gcgacgaaag cctggcggat ccggcgccct tcggcccgcc
      541 ggatcggaat gttctgcaga ttcggatcgg ccgaggacaa tctgccggtg gccgcgactg
      601 cctggtgata ggaggtatgg atgcgcccgg tgcgcggatc caccagctcc ggcaacttgt
      661 ccgtgtaggt tgatttgagc ttggccagca cccggtagtc gagcacgatc cgcgggagcg
      721 cgtaggagga ggcgagctcc tcgagaacgt cctcggccgt cgagggctgg ccggtcggcg
      781 tgcggcgcgt cacgggcagc tgcagcttct cgaacaggat ctgctgcagc tgccgcggag
      841 aatcgatgtt gaattcggcg cccgcctcgc gccgggcctg ctcgatgagc tcctgcagct
      901 gatggttgat ctcgcgagtc tgtgcgcgca gtcgctcgcg atcgaccagg acaccgcgcc
      961 gctccatccg cagcaggacc ggcatcaacg gccgctcgac gttctcgtac agctgcctca
     1021 gtttcggctc ggcctcgagc tggggccaca gcgtgtgatg cagacacaga gcgatgtccg
     1081 cctcctcggc tgcgtagcgg aaggcgctgt ccaccgggac ctggttgaag ggcagctgct
     1141 tggcgccctt gcccgccaca tcctcgtagg tcagcgtccg cataccgaga taacacaggg
     1201 cggtggagtc cacgtcgtgc ctggtggcca cgctgttcag cacataggac tcgagcatcg
     1261 tgtcgaagcg catcccggcg agctcgacgc cgtagttggc cagcacgtga gcgtcgtatt
     1321 tgaggtgatg acctatcttg ccgcgcgccg gatcctccag gatcggcttg agagccgcca
     1381 gcacccgatc gcggtcgagc tgctcgggcg ccccggcata gtcatgacgt aagggtacat
     1441 aggcagccac accgcgctgc agagcaaagg agacaccgac gatttcggcc ttcatgtagt
     1501 cgagtgtcga cgttgcggtg tcgaaggcaa acagctccgc agccccgagc atgccgatcc
     1561 agcgctccag ctcggcccag gtcgtgacgc attcgtagtg cctttcaact tgtgccagag
     1621 gcgactgctc gccggacccg gcaggcacgg cgctcgaagc cggctcggcg gctgtttcgg
     1681 gcgcagtgcc ctccagctgc ttcagaaagg agcgcagctc gtagcgcgtg tacagctcgc
     1741 gcagccgtgc ggtgtccggt gcagtcggca tcagctgctc gtgtgacagc ggcagatcga
     1801 gatcggtgcg gatcgtggcg agccggcgcg acagctcgag tacttccagt cctgcgcgga
     1861 tgttttcccc cacccggccg ctgatctgat cggcatgcgc gatcacgctg tcgagggcct
     1921 tgtactgctc cagcagcctc gccgccgtct tcggtcccac cttgtcgatg cccggaatgt
     1981 tgtcggagct gtcgccgatg agtgcgaggt agtcgatgat ctgctccgga tagacatcga
     2041 actttgcctt gacgccctcg cgatccagag ccgtgttgct catcgtgttg atcagcgtga
     2101 tggagccgtc cacgagctga gccatgtcct tgtcactggt ggagatcaga acgcgcaatc
     2161 ccgcgcgcga ggcgcggcag gcgagcgtgc cgatgacatc atccgcctcc actccctcga
     2221 tccgcaggat cggcacgccc tgggcgcgca ggatgctcag cagcggctcg atctgggggc
     2281 gcagatcgtt gggcattggc gtgcgatgcg ccttgtattc ggcaaacaga tcgtcacgga
     2341 aagtccgccc cgcggcatcg aagaccacgg caatgcgctg gggctgataa tccttcagga
     2401 acttgagcag catgttgagc acgccataga cggcgcccgt cggctcgccc cgcgagttgg
     2461 tcagcggcgg cagggcatgg aatgcccggt agacataaga agagccgtcg acgaggacca
     2521 gatcaggcga ttgggtcatg aagggggccg gcgcgggggg aagcccagta tatcggaccc
     2581 gtcaggggat tcggggggcc ggtccgtcaa cgcgtgctgt gctggcgcgc tattgcggaa
     2641 ggctcatcgg aggaggtctg cctttgcagc aagccgcggc tgattccctg ccggccgtag
     2701 cggccaaccg cttcggcctg ggcgcacggc ccggggagct gggccgcatc ggcgcagacc
     2761 cacgcgagtg gctgcgtggg caactcgccc aagcgccccc cctgctgatc gatgcgaatc
     2821 tgcagagctc ggccgagacg ctcgcgaagg cactcgaagc gcggcgtgag ctgcgtcagg
     2881 ccaagcgctc tgcagaggcc gagcgcacgg gcaatgggga ggcgcaggca gatgccctcg
     2941 ccgccctgct caaggtgggc cagatttatc gaccggtcta catggaggaa gcccgcgctc
     3001 gcttccgtga gggagtcgct accgatcgtc ccttcatcga gcggctcacc tatttctgga
     3061 cgaatcactt tgccgtctcg gtggataaga acatcgtgct ggggcttgcc ggcagcctgg
     3121 agcgcgaggc cgtgcggccg aacgtgctgg gacatttcgc cgatctgctg ctggccgtcg
     3181 agcagcatcc ggcgatgctg ctgtatctcg ataatcacgc ctcggtgggc ccccactcgc
     3241 aggcggcccg tgccgccgag agacgcaatc cggggcgaga gatcggcatc aacgaaaacc
     3301 ttgcgcgcga gatcatggag ctgcacaccc ttggagtggg cggtggctac acgcagcggg
     3361 atgtcacgac tttcgcggaa gtcctcaccg gctggtcgat aggcggatct catggtggcc
     3421 tctccggcgg agagccgggt cgtttcatgt tcaggccaca gctccacgag ccgggcgcca
     3481 aggtcgtgct cggacggcgc tatccggagg acgggttagg acagggtgtg gcggtgctgc
     3541 gccatctcgc tgcgcaccgc tctactgcac acttcatagc cacgaagctg gctcggcact
     3601 tcattgcgga cgatccgcca acgggggcgg tccagcggat cgctgccgcc ttcacaggca
     3661 gcggtgggca cttgccgacc gtttaccgtg cgctcatcga ttcgcccgag gcgtgggtcg
     3721 cgccactttc gaagttcaag acgcccaacg attacattct ctcgacattc cgcggcctcg
     3781 agctcccggt ggatgcgggc cgcgcgccgc tagctccatt cgaggtcatg gggcagcgca
     3841 cctacagtcc cggatcgcct gcgggttggc ctgatcgcag cgcagattgg gacggcgcct
     3901 ccgcactcat caagcgtctc gaatgggcag atgcgattgg acagcgcctc ggagcgcggc
     3961 gcgacgcggt agcccttgct tcccagctgc tcggtggcac gctcgaggag gggacgcgga
     4021 cagcgatcgc gcgcgctgcg agtgctgcac aggctctcac tttgttactc gccgcgccgg
     4081 agtttatgcg gcgctgagcg cagcttgcgc cgctatccca ggagaggtcc atggcaagct
     4141 tgaaacgacg ggaattcctg caggcgggtg ccctcgcctt gggcgggtcc ctcatgacat
     4201 cacgtctggc gtttgccggt atcggcgacg gaggcgactc ccgcgtcgtg ttcgtgatca
     4261 tgcgcggagc tctggatggt cttgccgcag tgcctcccta cggcgactcc gattacgcgg
     4321 ctctccgtcg tgagctgacc gttggggttc caggcgctgc ggacggcgct ctgcctctcg
     4381 atggcttctt cgggttgcat ccgcatctgg ggtttctgcg cgacacgtac agtgccggag
     4441 agttgatcgt ctttcacgcc gtggcgagcc cctatcgtga gcgctcacac tttgacggtc
     4501 aggatgtgct ggaaaacggc tcggtccatc cgcacgccct acagaccgga tggctcaacc
     4561 gggccctcgc ctcactgcag gccggccatg ctgggccgaa agagttgggc gtcgttttgg
     4621 gccagaacgt gccgctcctc atgcgcggtc cggcttcggt gacctcgtgg tcgccatcga
     4681 agctggcgac gctcgacgat gacacactgc agcgcgtaac cgatctgtac gctgccgacc
     4741 cgttgctctc gaagcgcctc gccgatgcgc tggcggcgga tgccatcgca gcgggttccg
     4801 actcgacgat gcaggggact gcagttgcgg cgaagccggc ccgctatgcc gagatcgttc
     4861 atgcggctgc cgggttcctg cggcgggatg acggacctcg cgtggcggtg ttcgatacga
     4921 ccggctggga tacgcatgcg aacgagggcg gcgcggaagg ttcgctcgcc gcaagactga
     4981 cggcactgga cgacggtttg cgcatgctga aggaagggct gggaccggtc tggagtcaca
     5041 ccgcggtgtt gcttgcgacc gaatttggcc ggaccgttgc gaccaacggg acacggggca
     5101 cggatcacgg cactgcgact gcggcctttc tggtcggcgg cgccgttaaa ggcggtcgtg
     5161 taatcgcgga ctggcccggc ctgtccgctc gggcgctcta tgagggccgt gacctcaagc
     5221 cgacgctcga tctgcgctcg gtactcaaag gagtcctggc ggagcacatg cgagtctcca
     5281 acggcgcact cgaggattgg gtttttcccg agagcaccaa ggcaacgccg atccagggcc
     5341 tgatgcgcgc ctgactcagc gtcgacgcga tgtcagttgc tgcgatcgcc ccgatgcggc
     5401 ggcgactgtg agttgtcatc gtcgtccttg cgctcatccg gcttctgcgg cggcgtgcct
     5461 tgagacgact ggttaggcga ggcttggtcc gacggcgccg gagcggacga aggcggaggt
     5521 gttgtggtct tctgaccttg cgtcggggcg gtgcccggcg cggcggagcc agctggacgg
     5581 gtgatcaccg ttccacgatc cggcaggtag agcgggccct tctggccaca ggcggccagc
     5641 gggagcaggc tgcccagtgc cgcgaggacg cccatccaac tcggaccaac acgctgcaag
     5701 actacaccgg gcgatgatcg cgtagcgcga gccagccgcg cgcgatgcgg tagatgaccc
     5761 agattcccag aatgacgtga ccgacccaca acgtcacgag ccccaccagg atgagcatca
     5821 acggcagcga gaccgcccag atcacgacca gccacagcag cgcgaaccag aaggtacgaa
     5881 tctgccagcg gaagtgacta tcgagatagg agccgcgggc gtcgcgctga cgcgcgtaat
     5941 tcatgatcac ggcgatgatc gacggaatcc cgaaaatgaa gctgcccgcg atcgtccaga
     6001 aggtagccag acccatcacc accgataagg tgtgcagggc gtagatgaca tgcgtgtagg
     6061 tgagaagcga gctgttcgct tctgcgctgg cagtcgtgct ggcgttcaag ttctcggcca
     6121 cagcgatact atgacatgtt cttcgggcga tccaccacgg ccatcgtcag ccgcgagaca
     6181 cacacgagcc tgtcctgctc gtcacggatc tcgatgtgcc agacctggct gcggttgccg
     6241 aggtggtagg gccgcgccgt cgccgtcacg atgccggaag acacgggccg gatgtgattc
     6301 gcgttgatct cctgacccag gcacacgtgt ttctgtgggt cgacgcacag gctcgccgca
     6361 aggctgccga cggtttccgc cagcgcaacg gatgcgccgc cgtgcaggac gcccatcggc
     6421 tggtgggttt gagggttgac cggcatcgtg gcgcgcacgt aatcaggtcc gacctccgca
     6481 actcggatgc cgatgtgct
//
LOCUS       DASRHN010000091         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1513861, whole genome shotgun sequence.
ACCESSION   DASRHN010000091 DASRHN010000000
VERSION     DASRHN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513861"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..859)
                     /locus_tag="VFB37_03435"
     CDS             complement(59..859)
                     /locus_tag="VFB37_03435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZO21532.1"
                     /translation="MHALLNIAVRAARSAGEIIIRSLNRLESLTITSKGRNDFVSEVD
                     RAAEQEIIKLIRRHYPSHAFLAEESGRAGESDTLWIIDPLDGTTNFLHGFPTFAVSIA
                     CQHKGRLEHGVVYDPISQEIFMATRGAGAHLDNRRIRVSKQRGLDGALIGTGFPYRAN
                     TRFMDCYLAMLKAVMEHTAGVRRPGAASLDLAYVAAGRTDAFWEIGLSPWDTAAGTLL
                     IQEAGGRIGTLAGGEYRQTGNVLAGTPKVYAAMLELLAPHLPPELREA"
     gene            922..1668
                     /locus_tag="VFB37_03440"
     CDS             922..1668
                     /locus_tag="VFB37_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZO21533.1"
                     /translation="MKRANIRIVLVEPSHPGNIGAAARAMKNMALEELVLVRPKAFPH
                     AEATARASGADDVLARARVVGSLAEALQGCGFVAATTSRDRDQNFRVADVREVAGRIV
                     SEAQRAPAAVLFGAERTGLTNEDLEAAHLLIRIPTSAVYASLNLAMAVQLVVYELFRA
                     QGARIIPLAGTAPLATPEEMDRLYLHLAQVLEEIDFRDRTQSGTHLMARIRRFLQRAE
                     LDQNEVNILRGMLTAMQSRRRKAGNPPPEA"
     gene            1665..2834
                     /locus_tag="VFB37_03445"
                     /pseudo
     CDS             1665..2834
                     /locus_tag="VFB37_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005090087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            2832..3305
                     /locus_tag="VFB37_03450"
     CDS             2832..3305
                     /locus_tag="VFB37_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZO21534.1"
                     /translation="MPAGAASPELPARQSTSIAAVRPASDLSELAWELFATLPGAGPL
                     SHAHASGAGTHVSGEAGSASEGTWVRFHLALEPGPSIERGTVKDARFQAYGCPHTLAT
                     AAWLTGRLRGRTREALVPGGPQDWMRALSVPIEKLGRLLIIEDALRACMQAWTGP"
     gene            3420..3743
                     /gene="iscA"
                     /locus_tag="VFB37_03455"
     CDS             3420..3743
                     /gene="iscA"
                     /locus_tag="VFB37_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HZO21535.1"
                     /translation="MAISLTESAADRVKTFLGARGRGVGLRLGVRKTGCSGFAYVINY
                     ADEVTPGDLMFEDRGVKVFVDPASLSLIDGTIVDFVKQGLNEAFRFRNPNVKGECGCG
                     ESFSV"
     gene            3847..>4229
                     /gene="ndk"
                     /locus_tag="VFB37_03460"
     CDS             3847..>4229
                     /gene="ndk"
                     /locus_tag="VFB37_03460"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZO21536.1"
                     /translation="MALERTLSIVKPDGVSRNLIGEVYRRFEKAGLRIVAARMLHLSS
                     REAEGFYAVHRERPFFKDLVRYMTSGPVIVQVLEGENAIARNREIMGATDPKKAAPGT
                     IRAEHALSIEQNVVHGSDAAETAAT"
BASE COUNT          687 a         1377 c         1427 g          738 t
ORIGIN      
        1 ggcgcgatgc gcaaccccgg gcacgaatcg ccgcccggca cacggatgtg cagtttgtct
       61 aggcttcgcg cagctcgggc ggcaggtgtg gagcgagaag ctcgagcatc gccgcataga
      121 ccttcggcgt gccggcgagc acgttgccag tctggcgata ctctccaccg gccagtgtgc
      181 cgatgcgccc gcccgcctcc tggatcagga gcgttcctgc ggcagtgtcc cacgggctca
      241 ggccgatctc ccagaaggca tcggtccgcc ccgccgcgac ataagccaga tcgagcgagg
      301 cggcgcccgg gcgccgcaca ccggctgtat gctccatgac ggccttgagc atcgccaggt
      361 aacagtccat gaagcgtgtg ttggcgcggt acggaaaacc agttccgata agcgccccat
      421 caagaccgcg ctgcttgctg acacgaatgc gccgattgtc caggtgcgca ccggctccgc
      481 gggtggccat gaagatctcc tggctgatcg gatcgtagac gacgccgtgc tcgagccgtc
      541 ctttgtgctg gcaggcaatc gagaccgcga aagtcggaaa cccgtgcagg aagttcgtcg
      601 tgccatcgag cggatcgatg atccacagcg tgtcgctttc gccagcgcgg ccgctctcct
      661 ccgccagaaa agcgtggctc ggataatggc gacggatgag cttgatgatc tcctgctcgg
      721 cggcacggtc gacctcgctc acgaagtcat tacggccctt ggaggtgatc gtgagtgatt
      781 cgagccgatt gaggctgcgg atgatgatct cgccggcgct gcgggcggcc cggacggcaa
      841 tattgagaag cgcgtgcacg ttaaataagc tctccgtggc cgccgggcca tcctgcaagc
      901 caaacaggca atcgtagcag attgaaacgc gccaacatcc gcatcgtgct ggtcgagcct
      961 tcgcacccgg gcaacatcgg ggcggccgcg cgcgcgatga agaacatggc gctggaggag
     1021 ctggtcctgg tgcgccccaa ggcctttccg catgcggaag ccacggcacg tgcctccggt
     1081 gccgacgatg tgctcgctcg cgcacgcgtg gtgggttcgc tggccgaggc cctgcagggt
     1141 tgcggcttcg ttgcggcaac cacctcacgg gatcgcgatc agaacttcag ggtagccgat
     1201 gtacgggaag tcgccggtcg catcgtgagc gaggcacagc gcgcgccggc cgcagtgctg
     1261 ttcggagccg agcgcaccgg actgaccaac gaggatctgg aggccgctca cctgctgatc
     1321 cggatcccca ccagcgccgt gtacgcctcg ttgaatctcg ccatggcggt ccagctcgtc
     1381 gtgtacgagc tgtttcgtgc ccagggggcc cggatcattc cgcttgcggg cacggcgccg
     1441 ctggcgaccc ccgaagagat ggaccgcttg tacctgcatc tggcacaggt gctcgaggag
     1501 atcgacttcc gtgaccgaac ccagagcggt actcacttga tggcccgtat ccggcgcttc
     1561 ctgcagcgcg cggagctgga tcagaacgag gtgaacatcc tgcgcggcat gctcaccgcg
     1621 atgcagagcc ggcgccggaa ggccggcaat ccgccacccg aagcatgagc gagccgatct
     1681 acctcgatta cgcggccacg acgccggtgg atccgatggt cgcgcagctg atgggcgagt
     1741 gcctggcgtc cgatggggac ttcggcaacg cggcctccgc cagccatctg ttcgggcggc
     1801 gtgctgcggc gcgcatcgag gccgcgcgcg cgcaggtggc agcgctcatt catgccgcgc
     1861 ccgaggaaat cgtgctgacc tccggtgcga ccgaatccaa caacctggcc attctgggaa
     1921 tcgcccgggc gagcgcgcat cggggccgcc acgtgttgac ttcgcgcatc gagcacaagg
     1981 cggtgctcga tccgtgcaga gagctgcaga aggagggctt cagcgtcacg tacctgagcc
     2041 cagaccactc cggcagcatt gccgcgcgag ccgtcgaggc ggcactgcgt cctgagaccc
     2101 tgctggtatc gctcatgctg gccaacaacg agaccggagt gctgcaggat ctcgtggcga
     2161 tcggcgctct gtgtcgggcg cgcggggtcg tgtttcattc cgatggggcc caagcggtgg
     2221 gcaagatcgc cgtggatgtg cgcgagcttc ccattgacct gctatccctg accgcgcaca
     2281 agctctacgg acccaaaggc gtgggtgccc tctacgtgcg ctcagcagcc cgggcgttgc
     2341 tgaagcctgt cagctttggc gggggccagg agcggggact gcgcccgggc acgctggcga
     2401 cccatcaggt cgtgggcctg ggcgccgcct gcgagctcgc cggccagtcg cagtcagtgg
     2461 agtcggcacg cctggcgggg cttcgcgatc gtctctggca caagctcgag gctctcggca
     2521 aggtccacct caatggcgag gaggctcccc gcctgcccgg actcctcagc gtctcctttg
     2581 agggcgtgga aggcgagagt ctcgtcaccg gcttgccgga gctggcggta tccacaggag
     2641 cggcctgcaa ttccgcctcg ggcgagccct cctacgtgct gcgggcgctg gggcgcgata
     2701 cgcagctcgc ccaggcgtcc ttgcgcttca gtctgggccg cttcacgacc gaagcggatg
     2761 tcgatctcgc ggcgagcgcc gtatgccgtg aggtccggcg gctgcgggat gtctctccct
     2821 tggcgtggcc gatgcctgcg ggggccgcca gcccggagct ccctgcgagg caaagcacct
     2881 ccatagccgc cgtccggccg gcctctgacc tcagcgagct cgcctgggag ctctttgcca
     2941 cgctgcccgg ggcagggcct ttgtcccacg cccacgcttc gggagccgga acgcacgtca
     3001 gcggcgaggc tggcagtgcg agcgagggga cctgggtccg cttccatctg gctctcgagc
     3061 cgggcccctc tatcgagcgg ggcactgtga aggatgcacg cttccaggcc tatggatgcc
     3121 cccatactct ggccacagcg gcctggctta cgggtcggct tcgcggacgg acccgcgagg
     3181 ccctggttcc gggcgggccg caggactgga tgcgagccct ctcagtcccc atagagaagc
     3241 tgggacgcct tctgatcatc gaggatgcgt tgagagcttg catgcaggcg tggacggggc
     3301 cgtgaggcag cgttccggac cggtggtttt agcgctggtg cccctcattg ggttcaaggc
     3361 gccccggcca accgtttccg tcctagaatg gatcctctca ccgtgaattt ctgaaagcga
     3421 tggccatctc tctcactgaa tccgccgccg atcgtgtcaa aaccttcctg ggtgctcgcg
     3481 ggcgcggggt cgggctgcgg ctcggggttc gcaagaccgg ttgctcgggt tttgcttacg
     3541 tcatcaacta cgctgacgag gtaacccccg gagatctcat gttcgaggac cggggcgtca
     3601 aggtgttcgt ggatcccgcc agcctctcgc tcatcgacgg cacgattgtg gatttcgtca
     3661 aacagggcct gaacgaggct ttccgcttcc gcaatcccaa cgtgaagggc gaatgcggct
     3721 gtggggagag cttttcggtc tgaccccccg caggtagact gccgggctgg tcagtctgac
     3781 ttgtcgcccc gcccgtgcga ttcgcgcggg ccggtctccc cgcagccctg ataaggatca
     3841 acgcgaatgg cgctcgagcg caccctgtcc atagtcaagc cggatggcgt cagccgcaat
     3901 ctcatcggcg aggtctatcg tcgtttcgag aaggccgggc tgcgcatcgt ggccgcccgc
     3961 atgctgcatc tgtcatcgcg cgaggcggaa ggcttttatg cggtgcatcg cgagcggccg
     4021 ttcttcaagg acctggtccg ctacatgacc tcggggcccg tgatcgtgca ggtgctggag
     4081 ggcgagaatg ccattgcacg caatcgcgag atcatgggcg ccaccgatcc gaagaaggcc
     4141 gctccgggca cgatccgcgc agagcatgcg ctgagcatcg agcagaatgt cgtgcacggc
     4201 tccgatgcag ccgagaccgc ggccaccga
//
LOCUS       DASRHN010000092         8151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_697360, whole genome shotgun sequence.
ACCESSION   DASRHN010000092 DASRHN010000000
VERSION     DASRHN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697360"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1723
                     /locus_tag="VFB37_03465"
     CDS             134..1723
                     /locus_tag="VFB37_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21537.1"
                     /translation="MINPEARPCDALAIRSARTDRACAQRSKKWVLAAAVLGSTMAFV
                     DESVVNVALPAMESDLAATLAAMTWVVNAYTLCMSALLLTGGAAADQLGRRRIFLAGL
                     CVFAVASLCCGLAPGALSLIASRAVQGVGAALLIPCSLAIIGAAFEGRERGAAIGIWS
                     GASAIAAGAGPLLGGWLVDHWSWRAIFLINPVIAVPTLWIAIRHLPESRDPSARRGLD
                     WIGALLAFTGLGSLAYGLIAAAHRSWRDTSVLVTVGAGVLLLLGFIAFERRSRAPMMP
                     LELFRSRGFSGVNLLTFLLYGALGGVMFFLPFLLIQVHGYSATAAGAAFLPFTVVLGL
                     LSKWGGGLVDRIGPRWPLIVGPLVVSAGFWLLSVPGPDGLYRSTFLLPMVVLGLGMAI
                     TVAPLTTTVLDAVPQHQSGTASGINNAVASVASLLLIALLGTIGSMKLSHSLEEHLLR
                     AQATPQVRQIVEQTKGGFVVPAMPPGISQETAQTAHAIIKAALIDAVKLVTLIGVALA
                     VASAAAAAALIGPAAERPQVP"
     gene            complement(1802..6019)
                     /gene="rpoC"
                     /locus_tag="VFB37_03470"
     CDS             complement(1802..6019)
                     /gene="rpoC"
                     /locus_tag="VFB37_03470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZO21538.1"
                     /translation="MKDLLKLFKQHAPVEHFDSIKIGLASPEMIRAWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVVCEKCGVEVTQSKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLMLDMTLREIERVLYFEAFVVIDPGMTPMQRGQLL
                     TDEMYLEAIEEHGDEFDARMGAEAVFELLRTIDLSQEMIKIREEMAGTSSETKLKRLS
                     KRLKLVESFIESGNKPEWMVLTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLRRLLELNAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPQLRLHQCGLPKKMALELFKPFIFQKLQLRGEAS
                     TIKAAKRLVEREGPEVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPLLVEGKAIQLH
                     PLVCTAFNADFDGDQMAVHVPLSLESQLEARALMMSSNNILSPANGDPIIVPSQDVVL
                     GLYYMTRERVGATGEGMAFSDVHEVHRAYESRSIDLQAKIKVRLRQQVRGEGGTTQDR
                     VRLVETTVGRALLFEILPKGLSFDLINQDMTKKAISATINACYRALGLKETVVFADQL
                     MYTGFHYATRSGVSFGIDDIVIPEQKTRIVASADREVKEIQDQYSSGLVTNGERYNKV
                     VDIWSRANDQVAKAMMEKLGTEEATDLRGQLKKQKSFNSIFMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEHDCGTSNGLSMAPLVEGGDVVEALGERVLGRVLAEDILKPGTEEV
                     LIAKNTLLDEQLVRRLEHDGVDQMMVRSPITCETRYGVCSSCYGRDLARGRSISIGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASSVEVRNKGVLRFHHVKTVQHEKGH
                     LVAVSRSGEIGVVDEYGRERERYKIPYGAMITAKEGDTVAGGQVVATWDPHTHPVVTE
                     VAGFLKFQDFIDGMTVTSQVDEVTGLSSTVVLDTKQRGGKDLRATMKLVNSKGKEVTF
                     ANTEIPAVYTLPPGALVSLEDGAKVSVGDVIARIPQESSKTRDITGGLPRVADLFEAR
                     KPKDPAILAEKSGTVSFGKETKGKRRLIITSDDGEKYEELIPKWRQLNVFEGETVERG
                     EVIADGEPNPHDILRLQGVEALANYLVREIQDVYRLQGVKINDKHIEVIIRQMLRKTE
                     VQEIGETTLLRGEQLDRARALDINDRSKQDGKQPARLQPVLLGITKASLATESFISAA
                     SFQETTRVLTEAAVRGLKDDLRGLKENVIVGRLIPAGTGFAAHASRRRKTEGTDRRSF
                     MDVGGGLPDIEESNVSEESESAASSST"
     gene            complement(6092..>8151)
                     /gene="rpoB"
                     /locus_tag="VFB37_03475"
     CDS             complement(6092..>8151)
                     /gene="rpoB"
                     /locus_tag="VFB37_03475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZO21539.1"
                     /translation="PFLEHDDANRALMGSNMQRQAVPTLRSETPLVGTGMERPVAIDS
                     GVTVVAKRGGSVDSVDASRIVVRVNDDETTAGEPGVDIYNLTKYTRSNQNTCINQRPL
                     VHAGDAIARGDVLADGPSTHLGELALGQNLLVAFMPWNGYNFEDSILISERVVQEDRF
                     TTIHIEELTCVARDTKLGPEEITADIPNVGDAALAKLDEAGIAFIGAEVKAGDILVGK
                     VTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPPGMDGTVIDVRVFTRDGVDKDSR
                     ALSIEKAEIERVRKDFADQQRILEDDLYQRVRGMLVGQSAAGGPRKLKASTRITAEYL
                     DELPREEWFEIRLEDEDANSQLEQTSERLKLQRKAFEKRLDDKKAKITAGDDLAPGVL
                     KMVKVYLAVKRRVQPGDKMAGRHGNKGVISTIVPVEDMPYLEDGTPVDIVLNPLGVPS
                     RMNVGQVLETHLGWAAKGVGLKIGRMLEAQSAVAELRAFIKRIYNETGGQKEETDSLS
                     DADLLQLARNLSGGVPMATPVFDGASEEEIKRLLTLADLPLNGQTHLFDGRTGERFER
                     QVTVGYMYMLKLNHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAY
                     GAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMDAGMPESFNVLVKEIRSLGINMEL
                     EQD"
BASE COUNT         1286 a         2728 c         2521 g         1616 t
ORIGIN      
        1 cgatgtgctg cgcagaccgg atgatccccg cagccagatc tcgtacaagg gaccggcact
       61 gggcaagacg gatcgtttcg tcctcgtatt tcgcaagacc tagccccggg ccggactcgc
      121 ggcggcggct gccgtgatca accctgaagc ccgcccctgc gatgcgctcg ccatccgctc
      181 cgcccggacg gaccgggctt gcgcgcagcg cagtaagaag tgggtgctcg ccgctgcggt
      241 cctcggctcg accatggctt tcgttgacga gtcggtcgtc aatgttgccc tgccggcaat
      301 ggagagcgat ctcgccgcga cgctggcggc gatgacctgg gtggtcaatg cctacaccct
      361 gtgcatgtcc gccctgctgc tcacgggtgg tgccgcggct gaccagctgg gtcgtcgccg
      421 catctttctg gcagggttgt gcgtattcgc cgtcgcgtcc ctctgctgcg gactggcgcc
      481 cggcgctctg tccctcattg cctcgcgtgc cgtccagggc gttggggctg cactcctgat
      541 cccctgttcg ctggcaatca tcggcgctgc attcgaggga cgagagcgcg gcgcagccat
      601 cggcatctgg tcgggcgcct cggcgatcgc tgcgggcgcg ggcccgctgc ttggcggatg
      661 gctcgtggat cactggtcct ggcgggcgat cttcctgatc aacccggtga tcgctgtgcc
      721 gacgctgtgg atcgcgatcc gtcacctgcc cgagagccgt gatccgagcg cgcgccgcgg
      781 actcgattgg atcggcgcgc tgctcgcctt taccggactc ggttcactcg cttacgggct
      841 gatcgccgca gcgcatcgca gctggcggga cacctcggtg ctcgtgacgg tgggagcggg
      901 tgtgctcctc ctgctcggct tcatagcctt cgagcggcgc agccgcgcgc caatgatgcc
      961 cctcgaactc ttccgatccc ggggtttcag cggcgtcaac ctgctcactt tcctgctcta
     1021 cggagccctg ggtggagtga tgttcttcct gcctttcctg ctcatccagg ttcacggtta
     1081 ttcggcgacc gccgcgggag ctgctttcct gccgttcacc gtcgtactcg gcctgctctc
     1141 gaaatggggc ggcggtctcg tcgatcgcat cggccctcga tggcctctca tcgtcggacc
     1201 cctcgtcgtg tccgcaggct tctggctcct cagcgttccg ggaccggacg gcctctaccg
     1261 ctcgaccttt ctgttgccga tggtcgtcct ggggctgggc atggcgatca cggtcgcacc
     1321 actcaccacg accgtcctcg atgccgtgcc ccagcaccag agcggcaccg cttccggaat
     1381 caacaacgcc gtggcctcag tggccagtct ccttctgatc gcattactcg gcaccatcgg
     1441 atcgatgaag ctgagccact cactcgagga gcatctgctg cgcgcgcaag ccacgccgca
     1501 ggtacgccag atcgtggagc aaacgaaggg gggtttcgtc gtgcccgcga tgcctccggg
     1561 gatttcgcag gaaacggcgc agaccgctca cgcgatcatc aaggccgcgc tcatcgatgc
     1621 agtcaagctc gtgaccctga taggggtggc gctggcagtt gcgagtgccg cagcggctgc
     1681 cgcgctcatc ggtcctgcgg ccgagcggcc gcaggttccc tgatacggca caacctggcg
     1741 aattccttcg cacacccgcg cggacagcgc gccgccactg cgggcggcgc gcatctgcat
     1801 ctcaggtgct gctgcttgcc gcgctctccg attcctcgct gacattcgat tcctcgatgt
     1861 cgggcaagcc gccgcccacg tccatgaagc tgcggcgatc ggtaccctcc gtcttgcgcc
     1921 ggcgcgaagc gtgagccgcg aagcccgtgc ccgccggaat gagccggccg acgatcacgt
     1981 tctccttcag accgcgcaga tcgtccttca gaccgcgcac cgccgcctcg gtcagcactc
     2041 gtgtggtctc ctggaaggag gctgcagaga tgaaggattc cgttgccagg gaggctttcg
     2101 tgatgccgag cagcaccggc tgcagtcgtg ccggctgctt accgtcttgc ttcgagcgat
     2161 cgttgatatc gagcgcccgc gcccgatcga gctgctcgcc acgcagcagc gtggtctcgc
     2221 cgatctcctg gacctcggtc ttgcgcagca tctggcggat gatcacctcg atgtgcttgt
     2281 cgttgatctt cacaccctgc agtcgataga cgtcctggat ctcgcggacc aggtagttcg
     2341 cgagcgcttc gacgccctgc agacgcagga tgtcgtgcgg gttgggctcg ccatcggcga
     2401 tgacctcccc gcgctcgacc gtctcgccct cgaagacgtt gagctgacgc cacttcggaa
     2461 tcagctcctc gtacttctcg ccgtcatcgc tcgtgatgat gaggcggcgc ttgcccttgg
     2521 tctccttgcc gaagctcacc gtgccggact tctccgcgag gatggccggg tccttgggct
     2581 tgcgtgcctc gaacagatcg gccacgcgcg gcaggccgcc ggtgatgtcg cgtgtcttgg
     2641 aggattcctg cgggatgcgc gcgatcacgt cgcccaccga aaccttggct ccatcctcga
     2701 ggctgaccag tgcgcccggc ggcaaagtgt agaccgccgg gatctcggtg ttcgcgaagg
     2761 tcacttcctt gcccttcgag ttcaccagct tcatggtggc tctgaggtcc ttgccaccgc
     2821 gctgcttcgt atcgagcacc accgtgctcg acaggcccgt gacctcatcg acctgcgagg
     2881 tcaccgtcat gccgtcgatg aaatcctgga acttgaggaa ccctgcgacc tccgtgacca
     2941 ccgggtgcgt gtgcggatcc catgtggcga ccacctggcc tccggccacc gtgtcgccct
     3001 ccttggcggt gatcattgcg ccgtaaggaa tcttgtagcg ctcacgctcg cgaccgtatt
     3061 cgtccaccac accgatctcg ccggagcggg aaaccgctac caggtggccc ttctcgtgct
     3121 gcacggtctt gacatgatgg aaacgcagaa cgcccttgtt acgcacctcg acgctgctgg
     3181 ctgccgccgc tcgcgatgct gcgccaccaa tgtggaaggt acgcatcgtg agctgcgtgc
     3241 cgggctcacc gatcgactgc gctgcaatga cgccgacggc ctcgccgatg ctgatcgagc
     3301 ggccgcgggc gagatcacgc ccgtagcagg acgagcacac cccgtaccgg gtctcgcagg
     3361 taataggtga acggaccatc atctggtcca cgccatcgtg ctcgagccgc ctcacgagct
     3421 gctcatccag cagcgtgttc ttcgcgatca gcacctcctc ggtgccgggc ttgagaatgt
     3481 cctcagccag tacgcgaccc agcactcgct ctccgagcgc ctccaccacg tcgccgccct
     3541 cgacgagcgg tgccatgctg aggccattgg aggtaccgca gtcatgctcg gtgacaacca
     3601 ggtcctgagc cacgtccacc agacgacgcg tcagatagcc ggagttggcc gtcttcagtg
     3661 ccgtgtcggc aagacccttg cgggcgccgt gggtcgagat gaagtactgc aggacgttca
     3721 ggccctcgcg gaagttggcc gtgatggggg tctcgatgat cgacccgtcc ggcttggcca
     3781 tgagaccgcg catgccggcg agctggcgga tctgcgctgc cgagccgcgc gcgcccgagt
     3841 ccgccatcat gaagatcgag ttgaacgact tctgtttctt gagctggccg cgcaggtccg
     3901 tggcctcctc ggttcccagc ttctccatca tcgccttcgc cacctgatca ttggcgcgcg
     3961 accagatatc gaccaccttg ttgtagcgct cgccgttggt cacgagacct gaggagtact
     4021 ggtcctggat ctccttcacc tctcgatcgg cggaggcgac gatccgggtc ttctgttccg
     4081 ggatcacgat gtcgtcgatg ccgaaggaga cgccggagcg cgttgcgtag tggaagcccg
     4141 tgtacatgag ttggtcggcg aagaccacgg tctccttcag accgagcgcg cgatagcacg
     4201 cattgatggt cgcggagatc gccttcttgg tcatgtcctg gttgatgaga tcgaaggaca
     4261 ggcccttcgg cagaatctcg aacagcagag cgcgcccgac cgtcgtctcc accagacgga
     4321 cgcggtcctg agtggtgccg ccctcgccgc ggacctgctg gcgcagacgc accttgatct
     4381 tcgcctgcag atcgatgctg cggctctcgt aagcgcgatg cacctcgtgc acatccgaga
     4441 aggccatccc ctcacccgtg gcacccacgc gctcgcgcgt catgtagtac aggcccagga
     4501 cgacgtcctg cgacggcacg atgatgggat cgccgttcgc cggcgacagg atgttgttgg
     4561 aggacatcat gagggcacgc gcctcgagct gcgactcgag agacagcggc acgtgtacgg
     4621 ccatctggtc gccgtcgaag tccgcattga aggccgtgca gaccagcgga tgcagctgaa
     4681 tcgccttgcc ctccacgagc agcggctcga aggcctggat gcccaggcga tgcagtgtcg
     4741 gtgcgcggtt gagcagcacc ggatgctcgc ggatgacctc ctcgagaatg tcccacacct
     4801 ccggtccttc gcgctcgacc aggcgcttgg ctgccttgat ggtcgaggcc tcgccccgca
     4861 gctgcagctt ctggaagatg aacggcttga agagctcgag cgccatcttc ttcggcaatc
     4921 cgcactgatg caggcgcaac tgcgggccga cgacgatgac ggagcgaccg gagtaatcga
     4981 cacgcttgcc cagcaggttc tgccggaagc ggccctgctt gcccttgatc atgtcggcca
     5041 gcgacttcag cgggcgcttg ttggtgcccg tgatcgcgcg gccacgccgg ccgttgtcga
     5101 gcagcgcatc gactgcttcc tgcagcatgc gcttctcgtt cctgacgatg atatcgggtg
     5161 cgttgagctc gagcaggcgg cgcaggcgat tgttgcggtt gatgactcgg cgatacagat
     5221 cattcaggtc cgaggtcgcg aaccgaccgc cgtcgagggg tacgagcggg cgcagatcag
     5281 ggggcagcac cggcaggacg gtcagtacca tccactccgg cttgttgccg gactcgatga
     5341 aggactcgac gagcttcaga cgcttggaca gcctcttcag cttggtctcc gaggaagtcc
     5401 ccgccatctc ttcgcggatc ttgatcatct cctgcgacag atcgatcgtg cgcagcagct
     5461 cgaacaccgc ttcagcgccc atgcgggcat cgaactcatc gccgtgctct tcgatggcct
     5521 cgaggtacat ctcatcggtc aggagctgac cgcgctgcat gggtgtcatg cccggatcga
     5581 tgaccacgaa ggcctcgaag tacagcaccc gctcgatctc gcgcagcgtc atgtccagca
     5641 tcaggccaat gcgcgaagga agcgacttga ggaaccagat gtgcgcagtc gggctcgcca
     5701 gctcgatgtg tcccatgcgc tcgcggcgca ccttcgactg ggtgacctcg acgccgcact
     5761 tctcgcagac cacgccgcga tgcttgagtc gcttgtactt gccgcacagg cactcgtaat
     5821 ccttcacggg cccgaagatc ttggcgcaga acaggccgtc acgctccggc ttgaacgtgc
     5881 ggtaattgat ggtttcgggc ttcttcacct cgccgtacga ccaggcgcgg atcatctccg
     5941 gcgaggcgag gccgatcttg atcgagtcga agtgctcgac cggagcatgc tgcttgaaga
     6001 gcttcagtaa gtctttcatc tagtctccta acggctcgta ttcgccgtct tcaccgaggt
     6061 cccgggatcg atcgatcttt cccgggggtc atcagtcctg ctccagctcc atgttgatgc
     6121 cgagcgagcg gatctccttc acgagtacgt tgaaggactc cggcatcccg gcatccatct
     6181 ggtgattgcc gtcgacgatg ttcttgtaca tcttcgtgcg gccgttcacg tcatcggact
     6241 tgaccgtgag catctcctgc agcgtgtagg ctgccccata ggcctccagc gcccagactt
     6301 ccatctctcc gaagcgctgg ccgccgaact gcgccttgcc gcccagcggc tgctgcgtga
     6361 cgagactgta ggggcccgtc gagcgggcgt gcatcttgtc gtccaccagg tggttcagct
     6421 tcaacatgta catgtatcca acggtcacct ggcgctcgaa gcgctcgccg gtgcggccgt
     6481 cgaacaaatg cgtctggccg ttcagaggca gatcggcgag tgtcagcagc cgcttgatct
     6541 cctcctcgct cgcgccgtcg aacaccggcg tggccatggg caccccgccg gagagattgc
     6601 gggcgagctg cagcagatcc gcatcgctca gcgaatcggt ctcctccttc tgcccgccgg
     6661 tctcgttgta gatgcgcttg atgaaggcgc gcagctcggc caccgccgac tgcgcctcga
     6721 gcatccgtcc gatcttcagc ccgacaccct tggcggccca gcccagatgc gtctcgagca
     6781 cctggccgac attcatgcgc gaaggcacgc ccagcggatt caggacgatg tcaacgggag
     6841 tgccgtcctc cagataaggc atgtcctcga ccggtacgat cgtggagatc acacccttgt
     6901 tgccgtggcg tccggccatc ttgtcgcccg gttgcacgcg gcgcttcacg gcgaggtaca
     6961 ccttgaccat tttgagcacg ccgggggcga gatcgtcgcc ggcggtgatc ttggctttct
     7021 tgtcatcgag gcgcttctcg aacgccttgc gctgcagctt cagccgctcg ctggtctgct
     7081 cgagctgcga gttggcatcc tcatcctcga ggcgaatctc gaaccattcc tcgcggggca
     7141 gctcgtctag atactctgcg gtgatccgtg tggaggcctt gagcttgcgc ggtccgccgg
     7201 cagcgctctg acccaccagc attccacgca cgcgctgata caggtcatct tccaggatgc
     7261 gttgctggtc cgcgaagtcc ttgcggacgc gctcgatctc ggctttctcg atggacaggg
     7321 cgcgcgagtc cttgtccacg ccatcccggg tgaacacccg cacgtcgatg accgtgccat
     7381 ccattcccgg cggcacgcgc agactcgtgt ccttcacatc ggaggccttc tcgccgaaga
     7441 tcgcccgcaa cagcttctct tccggcgtga gctgggtctc tcccttcggc gtgaccttgc
     7501 ccaccaggat gtcccccgcc ttgacctccg cgccgatgaa ggcaatgccc gcctcatcca
     7561 gcttggccag cgcggcatct ccgacattgg gaatgtcggc tgtgatctcc tcgggaccga
     7621 gcttcgtatc gcgagccacg caggtcagct cctcgatgtg gatcgtcgtg aagcggtcct
     7681 cctgcaccac gcgctcggag atgaggatcg agtcctcgaa gttgtagccg ttccacggca
     7741 tgaaggcgac cagcaggttc tgaccgaggg cgagctcacc caggtgggtg gatggcccat
     7801 cagcgagcac atcgccccgc gcaatcgcat cgccggcatg caccagagga cgctgattga
     7861 tgcaggtgtt ctggttcgag cgggtgtact tcgtgaggtt gtagatgtcc acgccaggct
     7921 cgcccgcggt ggtctcatca tcgttcaccc gcaccacgat gcgggatgcg tcgacggagt
     7981 cgacgctgcc gccacgcttg gcgacgacgg ttacgcccga gtcgatggct acaggccgct
     8041 ccatgcccgt tccaaccagc ggcgtctcgg aacgcaaagt cggcacggcc tggcgctgca
     8101 tgttcgaacc catgagcgcg cgattggcat cgtcgtgctc gaggaaggga a
//
LOCUS       DASRHN010000093         5943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_196931, whole genome shotgun sequence.
ACCESSION   DASRHN010000093 DASRHN010000000
VERSION     DASRHN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196931"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..432)
                     /locus_tag="VFB37_03480"
     CDS             complement(22..432)
                     /locus_tag="VFB37_03480"
                     /inference="COORDINATES: protein motif:HMM:NF008769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HZO21540.1"
                     /translation="MSSAPPPLAKRPYLLRAMHQWMSDSGHTPHVIVDADRAGPELPK
                     AYVKDGKIVLNLSSSATQRLRLGNEDIEFDARFAGVVHHVKFPVSAVMGIYARETGEG
                     MVFSEQDLGPEPPKGPTSAEEGSGSRRPQLKVVK"
     gene            complement(444..1046)
                     /locus_tag="VFB37_03485"
     CDS             complement(444..1046)
                     /locus_tag="VFB37_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZO21541.1"
                     /translation="MTLFSAPNDPWSHRTRIVLAEKGISIDIVSVEPGKLPEDLLDLN
                     PYHSVPTLVDRDLVLYDSRVIIEYLDERFPHPPLMPVDPVTRAQFRLALYRIERDWYA
                     LVQQIEQEPDKKQTVKLKKILRDTVLQSTELFRVKPYFLSDEFSLVDATIAPVLWRMP
                     YYEIDLPPQAQPIIKYAGLIFSRPAFRESLSDTEQEMRLI"
     gene            complement(1090..1863)
                     /locus_tag="VFB37_03490"
     CDS             complement(1090..1863)
                     /locus_tag="VFB37_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HZO21542.1"
                     /translation="MRTKLLLLSALLWASGAALAAESGAEGASDWSHWHAGNDVKNIG
                     SLQRGARNFMNYCVGCHSLKYERYSRMAEDLEIPPDLLQKYLLRPGVKATQYILTSMP
                     AGDAETWFGKTPPDLSLMARERGVDYLYQFLKTFYVDPSRQTGANNLRLPSTAMPDVI
                     SALEGLKKPVYKNEHKGGEGGTAGSEQVVDHFEQIAPGEMNADEFDGFVRDTVNFLDY
                     VGEPAQVQRRELGVWVVLFLLVFTWLAWLLKREYWKDVH"
     gene            complement(1853..3145)
                     /locus_tag="VFB37_03495"
     CDS             complement(1853..3145)
                     /locus_tag="VFB37_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HZO21543.1"
                     /translation="MASLHEPDVRAPGDPLPPGSNPLPRKGFWAWINKRMPVDEFIAD
                     QLTGYYAPKNFNIWYFFGSLALLVLVVQLVTGIFLTMFYKVGETTSFDSVEYIMREVD
                     WGWLIRYLHSTGASAFFIVVYLHMFRAFLYGSYKPPRELLWILGMLVYLALMAEAFMG
                     YVLPWGNMSFWGAQVIVNLFGTIPGIGPGLVEWIRGDYGIADATLNRFFSLHVVAIPL
                     ALLLLVMLHLVALRRTGSNNPDGIEIKEKVGPDGKPLDGIPFHPYYTVKDVVGVGVFL
                     VVFAIIVFFTPTFGGLFLEGPNFGPANPMSTPEHIAPVWYFTPYYAILRAVPNQRMGA
                     LLMALAVVSFLFVPWLDRSPVKSMRYRGWISRLALAIFAISFIALGYLGLQPAEGSYV
                     LLARIFSVLYFAFFWLMPIYSRIDRVKPVPDRVIFHAH"
     gene            complement(3145..3765)
                     /gene="petA"
                     /locus_tag="VFB37_03500"
     CDS             complement(3145..3765)
                     /gene="petA"
                     /locus_tag="VFB37_03500"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZO21544.1"
                     /translation="MTENVVAADDGVDQSRRKFLTTATIATGAVGAVFAAVPFIASWQ
                     PSERAKALGAPTVLDISKLDPGQMTITSWRRSPIYVVRRTPEMLASLKGHDQLLKDPN
                     SEDSDQPPYTKNVYRARKPEVLVLIGTCTHLGCLPKQHFAPHDPEIGAYWPGGFFCPC
                     HGSRFDLSGRVFTGSPASINLRIPPYEFSNPNTLVVGLDETHKGAA"
     gene            3934..5076
                     /locus_tag="VFB37_03505"
     CDS             3934..5076
                     /locus_tag="VFB37_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZO21545.1"
                     /translation="MLERLGRGLTFVAGSVVGGLALAFLIVAARPELIRRSGAKAPVP
                     AVADPPTSVHAPARVTYADAVQRAAPSVVNVYTARLVTERVPPSSLGELFGGFLPRYR
                     QRVERSLGSGVIIDDSGHIVTNHHVIANADAIRVQLADGRVADAHIVGRDPDTDLAVL
                     KIDLSPLPTAVLGRSDQLKVGDVVLAIGNPVGLSQTVTHGIVSATSRQQLGLAALEDF
                     IQTDAPINVGNSGGALVDSSGALVGINTAIVAKDLGVEGIGFAIPVNMVRGVVSDIIA
                     TGRVIRGWIGIEPQDISDQQAAQLGLAQGGVVIANMYVGSPAQQAGLRPGDLILSVDG
                     TQVRSSQDVMGRIATHKPGSSLAIHALRGDHPFDVTAQVTERPRNS"
     gene            5122..5799
                     /locus_tag="VFB37_03510"
     CDS             5122..5799
                     /locus_tag="VFB37_03510"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZO21546.1"
                     /translation="MTTIQPRRFPTLAAFEQALTERLQGALGNPEPGRAAVMLSGGRT
                     PLPVYQELARKPPRPAPALRLLYSDDRYVPATSEASNYYQSRSLVDALRLPETAVLRV
                     RTELPLEQAAADYEQQLAALLRSGERIGLGLLGLGADGHTASLFSASDLEQARGRLAI
                     AVQRPDGLHGISVTPEVLSGIEELLFLVTGPDKHAIVEAFLKRDSNLIARRAVANCAN
                     VQIWIAE"
BASE COUNT         1091 a         1928 c         1907 g         1017 t
ORIGIN      
        1 gaatgctgta cgcgaccgtt tctacttcac gaccttcaat tgcggacggc gactgccgct
       61 gccttcttcc gcagaggtgg gtcccttcgg cggctcgggt ccgaggtcct gctcggagaa
      121 caccattccc tctcccgtct cgcgcgcata gatccccatg acggcgctca cgggaaactt
      181 gacgtgatgc acgacccccg cgaagcgggc gtcgaattcg atgtcctcat ttcccagccg
      241 cagccgttga gtcgcgctgg agctgaggtt gagaacgatc ttgccgtcct tgacgtaggc
      301 tttgggtagc tcaggacctg cccggtccgc atcgacgatc acatgcggag tgtgcccgct
      361 gtcggacatc cactggtgca tcgctcggag caggtaggga cgcttggcta aaggaggcgg
      421 tgcggaggac atgactcgca atatcagatc agacgcattt cctgctcggt atccgaaagg
      481 ctctcgcgga acgccggccg ggaaaaaatc agcccggcat acttgatgat gggctgagcc
      541 tgcggcggca ggtcgatctc gtagtacggc atgcgccaga gcaccggcgc gatcgtcgca
      601 tccacgagcg agaactcgtc ggagaggaaa taaggtttca cccggaacag ctccgtgctc
      661 tgcagcacgg tgtcccgcag gatcttcttc agcttcaccg tctgtttctt gtccggctcc
      721 tgctcgatct gctgcacgag cgcgtaccag tcgcgctcga tccgatacag cgccaggcgg
      781 aattgcgccc gcgtcaccgg atcgaccggc atcaggggcg gatgcggaaa acgctcgtcc
      841 aggtactcga tgatcacccg tgagtcgtac agcaccagat cgcgatccac gagtgtggga
      901 acgctgtgat agggattgag atcgagcaga tcctcgggaa gcttcccggg ctcgacgctc
      961 acgatatcga tgctgatgcc tttctcggcc agcacgatcc gcgtccgatg actccagggg
     1021 tcgttgggcg cggagaacag cgtcatgatg gatcgtctgc tcgacaagat tccgctcccg
     1081 gaactgcgat cagtgaacgt ctttccagta ttctcgcttc aacagccagg ccagccaggt
     1141 gaagaccagc aggaacagaa cgacccacac gccgagctca cgccgctgca cctgcgccgg
     1201 ttcgcccacg tagtccagga aattcaccgt atcgcgcacg aagccgtcga actcgtctgc
     1261 attcatctcg cccggcgcga tctgctcgaa atgatcgacg acctgctcgg agccggccgt
     1321 gccgccctcg ccgcctttgt gctcgttctt gtacaccggc ttcttcaggc cctccagtgc
     1381 ggagatcaca tcaggcatcg ctgtcgaggg cagccgcaga ttgttcgcac ccgtctggcg
     1441 cgaagggtcc acgtagaagg tcttcaggaa ctgatacaga tagtccacgc cccgctcgcg
     1501 cgccatgagc gacaggtcgg gcggggtctt gccgaaccag gtctccgcat ccccggccgg
     1561 catgctcgtg aggatgtact gggtcgcctt cacgcccggc cgcagcagat atttctgcag
     1621 cagatccggc gggatctcca gatcctcggc catgcgcgag tagcgctcgt acttgagcga
     1681 atggcagccc acgcagtagt tcatgaaatt acgcgcgccc cgctgcagcg agccgatgtt
     1741 cttcacatcg ttgccggcat gccagtggct ccagtccgag gcgccctcgg ctccggactc
     1801 cgccgccaga gcggcgccgg aagcccacag gagcgcactc aacagcagca gcttagtgcg
     1861 catggaagat cactctgtcc ggcaccggct tcacgcgatc gatgcgggaa tagatgggca
     1921 tgagccagaa gaacgcgaag tacaggacgc tgaagatccg cgccagcagc acgtacgagc
     1981 cttcggccgg ctgcaggccg aggtagccga gcgcgatgaa cgagatggcg aagatcgcca
     2041 gcgcgagccg cgagatccag ccgcggtagc gcatcgactt gacgggcgag cggtcgagcc
     2101 acggcacgaa cagaaacgac acgaccgcga gcgccatcag cagcgcgccc atcctttgat
     2161 tgggcacggc gcgcagaatc gcgtagtacg gtgtgaagta ccacaccggg gcaatgtgct
     2221 ccggggtgga catggggttg gccgggccga agttcgggcc ttcgagaaac aacccgccga
     2281 aggttggtgt gaagaacacg atgatcgcga acaccaccag gaacaccccg acgccgacga
     2341 cgtccttcac cgtgtagtac ggatgaaacg ggatgccatc gagcggcttg ccatccggtc
     2401 cgaccttctc cttgatctcg atgccgtcag ggttgttcga gccggtgcgc cgcagggcca
     2461 ccaggtgcag catcaccagc agcagcagcg cgaggggaat tgccacgacg tgcaacgaga
     2521 agaatcgatt cagggtggca tcggcgatgc cgtagtcgcc ccggatccac tcgacgaggc
     2581 cgggaccgat gcccgggatc gtgccgaaca ggttcacgat gacctgcgcg ccccagaatg
     2641 acatgttgcc ccagggcagc acgtagccca tgaaggcctc agccatcagc gccagataga
     2701 ccagcattcc gagtatccac aacagctcgc gcggcggctt gtacgagccg tagaggaacg
     2761 cgcggaacat gtgcagatag acgacgatga agaacgccga ggcgccggtc gagtgcaggt
     2821 agcggatgag ccaaccccaa tccacctccc tcatgatgta ctcgacggag tcgaacgagg
     2881 tcgtctcgcc gaccttgtag aacatcgtca ggaagatgcc cgtgaccagc tgcaccacca
     2941 gcaccagcag cgcgagcgag ccgaagaagt accagatatt gaaattcttc ggggcgtagt
     3001 acccggtgag ctggtcggcg atgaactcat cgaccggcat gcgcttgttg atccaggccc
     3061 agaagccctt ccggggcagc gggttgctcc cgggaggcag cggatcaccc ggcgcgcgca
     3121 cgtccggctc atgcagagag gccatcacgc ggctcctttg tgagtctcat cgagcccgac
     3181 gaccagcgtg ttcgggttgg aaaactcgta cggcggaatt ctcaggttga tcgaggcggg
     3241 cgagccggtg aagacgcggc ccgacaggtc gaagcgcgat ccgtggcagg gacagaagaa
     3301 gccgcccggc cagtaggcgc cgatctccgg atcgtggggc gcgaagtgct gcttgggcag
     3361 acagcccaga tgcgtgcagg tgccgatcag caccaggacc tcgggcttgc gcgcacggta
     3421 gacgttcttg gtgtacgggg gctgatcgga atcctcggaa ttggggtcct tcagcagctg
     3481 atcgtggccc ttgaggctag cgagcatctc aggggtccgg cgcacgacgt agatcggcga
     3541 acgccgccac gaagtgatgg tcatctgtcc cggatcgagc ttggagatat cgagcacggt
     3601 cggggccccc aaggccttgg cccgctcgga gggctgccag gaggcgatga agggtacggc
     3661 cgcgaacacc gcgcccaccg ctccggtggc gatggtggcc gtggtcagaa acttgcgtcg
     3721 actctgatcg acaccgtcgt cggcagcgac aacgttttcc gtcatcgaac gtttctcctc
     3781 tcacccgcgc atcgctcacc gcgcgcaatg gaggcatgca cggcagcagt ttccggcttg
     3841 cggggggcgg cgcattatac acaaacgaac tcgaccttcc gtgcaggcgg acaccgcaaa
     3901 gtaccgccgc gagcgcgtct tccggagtga accatgctcg agcgcctggg ccgcggactg
     3961 acgttcgtgg ccggatcggt agtgggcggc ctcgccctcg ccttcctcat cgtagctgcg
     4021 cgaccggagc tcatccgccg cagcggcgcc aaggcacccg tgcccgcggt ggcggatccg
     4081 cccacatcgg tgcacgcgcc ggcccgggtc acctatgcgg acgcggtcca gcgcgctgct
     4141 ccctcggtgg tgaatgtgta caccgcccgc ctggtgaccg agcgtgtccc gccctccagc
     4201 ttgggtgagc tgttcggtgg ctttctgccc cggtaccggc agcgcgtgga gcggagcctg
     4261 ggctcgggtg tgatcattga cgacagcggt cacatcgtca ccaaccacca cgtgatcgcc
     4321 aacgcggatg cgatccgggt tcagctggcg gacggacggg tcgccgatgc gcacatcgtc
     4381 gggcgcgacc cggacactga cctggctgtg ttgaagatcg atctctcccc gctgccgacc
     4441 gcggtgctcg gccgctccga ccaactcaag gtgggcgatg tcgtgctcgc catcggcaac
     4501 cctgtcgggc tctcccagac cgtcacgcac ggtatcgtga gcgcgaccag ccggcagcag
     4561 ctcgggctcg ccgcgctcga ggatttcatc cagaccgatg cgccgatcaa cgtcggcaat
     4621 tcgggcggcg cactcgtgga ctcgagcggg gccttggtgg gcatcaatac ggccatcgtg
     4681 gccaaggatc tgggcgtcga gggcatcggc ttcgcgatcc cagtgaatat ggtgcgcggt
     4741 gtcgtgagtg acatcatcgc cacgggccgg gtcatccgcg gctggatcgg catcgagccg
     4801 caggacatct cggatcagca ggctgcgcag ctggggctgg cacaaggggg cgtggtgatc
     4861 gccaatatgt acgtgggcag ccctgctcag caggccggtc tcagacccgg agatctgatc
     4921 ctctcggtcg atggcaccca ggtgcgcagc tcccaggatg tgatgggacg gattgcgacg
     4981 cacaagccgg gctcgtccct tgcgatccac gctctgcgcg gcgaccaccc gttcgatgtc
     5041 acggcacagg taaccgagcg gccgcgcaac tcctgatcct cgagctgccg cggcagcccg
     5101 gttcccttca tcggagcacc catgaccacc attcagcccc gccgcttccc caccctcgcg
     5161 gccttcgagc aggcgctcac cgagcggttg caaggggccc tgggcaatcc agagccgggc
     5221 cgggccgccg tgatgctttc aggtggccgc acgcccttac cggtatacca agagctcgca
     5281 cgcaagccgc cccgcccagc ccccgccctc cgcctgctct actcggacga ccggtacgtc
     5341 cccgccacat ctgaagcgag caactattac cagtcccgct cactggtcga cgccctgcgc
     5401 cttcccgaga ctgccgtgct gcgcgtcaga accgagctgc ccctcgagca ggccgccgcg
     5461 gactacgagc agcagctcgc cgctttgttg cgctccggtg agcggatcgg attggggctg
     5521 ctcggcctgg gcgccgatgg ccacacggca tcgctcttca gcgcgagtga cttggagcag
     5581 gctcgcgggc ggttggcgat tgcggttcag cggccggacg gcttgcacgg gatcagtgtg
     5641 acccccgagg tgctctccgg gattgaggag ctgttattcc tcgtcacggg gccggacaaa
     5701 catgccatcg tggaggcctt cctcaagcgg gactcgaatc tgatcgcgcg ccgcgcggtg
     5761 gccaactgcg ccaacgtgca gatctggatc gccgaatagc tacggtgcga atgcgctcag
     5821 cttgagcggc ccgcacgcgc gggtccaggt atgcgcatcg cttcccggat tgtgccagct
     5881 gcagtgcccg gcgatcagat cgtcggcctc tttggggccc caggatccgg gcatgtattc
     5941 gta
//
LOCUS       DASRHN010000094         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1514697, whole genome shotgun sequence.
ACCESSION   DASRHN010000094 DASRHN010000000
VERSION     DASRHN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514697"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFB37_03515"
     CDS             <1..516
                     /locus_tag="VFB37_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21547.1"
                     /translation="YTVAGAATMSAVFPGVGAVSVLATGWLSDRLGVNGRSLVMFVGL
                     GATTLALLFLMSLQAGSAEAWLPVLMIGIIAFCLLGPYSYLGGAFALDFGGKQASAIS
                     SGIIDGVGYLGGVVAGDSVARISVSYGWQGVFVALAGVTALTALGAGYLLLLGLRTSA
                     ALQAQTPPGGR"
     gene            519..1067
                     /locus_tag="VFB37_03520"
     CDS             519..1067
                     /locus_tag="VFB37_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO21548.1"
                     /translation="MSLADEVIAIYQIRGTEAYFGERVSMTEHALQAAFFARQADAPP
                     ELVLAALLHDIGHLLEAAPEFEQWSVDARHEEVGSRWVGRRVRPEVAGPILLHVAAKR
                     YLCATDADYIRMLSPASVVTLKLQGGPMTSPEISAFEAEPAFRNAVRIRHWDDQGKVA
                     GLRTPGLADYRELIDSFAVRNT"
     gene            1156..3603
                     /locus_tag="VFB37_03525"
     CDS             1156..3603
                     /locus_tag="VFB37_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZO21549.1"
                     /translation="MRAQSWIIGAWAMLATVAASSAVPPPRAANAVVPAASAPRLHLA
                     GVRSLQQRLSGSTAKLDSALADLSGHLSQVRSDHALEDLYALNPAARFMQRSAAEGPL
                     VLVDAVTTGDPQKLKSALVSLGMQHAAVYSNDVGGFLPLAQLANAAARAEVHFMRASM
                     PRTRIGAVTSQGDFAQHSDVLRSTYSALNGTGVTVGVMSDSYDCYDVYAEPGSKVPVS
                     GPTGYAPNGFTASATTDISTGDLPSSVALVATSNAGEFPCLSYGAPTLPPFSDEGRAM
                     LQIVHDVAPGASLAFHTAEWSEADFASGVTALAAAGAKVIADDVGYFDEPFYQDGIIA
                     QAIDTVEGKGVAYFSAAGNSADHAWESTNPTFVALPSPPVPGELLVNFDTTGATKKTT
                     LSVDIPGLQQGELLAVVVEWDQPYVTGAPHSGGATSHIDLCISGAGANTIINTSGVAE
                     GCTGANATGSDPVQVLIVDNPANAPAVTAATTLNITVGLLDGTAPPGRIRVAVDDGGA
                     GATIDPTVATHSSTMQGHPNAAGAAAIGAAAFFSTPACGTSPAVLEKYSALGSVQLPI
                     LFDSAGTRLATPVLRQKPDFVGPDGGDNTFLGFTLAKAGADFQDHSTIAQCLDNTSYP
                     NFFGTSAATPHAAAIAALMLQANPALTPTQIYTALRTSALPMSGTTPDPSSGSGFIQA
                     DAALKLLPPGPPALSFSASSVMTGGTTTLSWSSINTSSCTASGSWSGAQKTSGSATIT
                     APSTAGDAAYTLTCTNSEGSANATVHLTVMAASSHGGGGGAVDELSLLVLAALGCLRL
                     LRPGRDSIVSPRRHRGP"
     gene            complement(3509..>3797)
                     /locus_tag="VFB37_03530"
     CDS             complement(3509..>3797)
                     /locus_tag="VFB37_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21550.1"
                     /translation="FYATLATLTGTLTLYDLWRKSRKEAVPRALKAPFITAQPQAMVG
                     QFLADADRKGAPPPAAEDASVRDLDASGATRLSPFPAAGVAGTPEPPERAG"
BASE COUNT          606 a         1239 c         1283 g          669 t
ORIGIN      
        1 tacaccgtag cgggcgccgc gaccatgagt gcggtgttcc ctggggtcgg agccgtgtcg
       61 gtgctcgcca ccggctggct gagtgatcgg ctcggggtga acgggcgctc gctcgtcatg
      121 ttcgtgggac tcggcgcgac cacgctcgcc cttctttttc tcatgtcgct gcaggccggc
      181 tccgccgaag cctggctccc cgtgctcatg atcggcatca tcgccttctg cctgctgggt
      241 ccctactcct acctcggagg cgcctttgcg ctggatttcg gcggcaagca ggcgagcgcc
      301 atctcctcgg gcatcataga tggtgtaggc tatctgggcg gtgtcgtggc aggcgatagc
      361 gtggcgagga tctccgtgag ctatggttgg cagggcgtgt tcgtggcgct ggcgggtgtc
      421 acggcgctga cggcattggg ggcgggctac ctgctgctgt tggggctgcg gacctcggca
      481 gccctgcagg ctcagactcc tccgggcggt cgctgagagt gagcctcgcc gacgaagtca
      541 tcgcgatcta tcagatacgc ggcaccgagg cctacttcgg cgagcgcgtc tcgatgaccg
      601 agcacgcctt gcaagccgcc ttctttgcgc gacaggccga tgctccgccg gagctcgtcc
      661 tcgccgccct gctgcatgac atcggacacc tgctcgaagc tgctccggag ttcgagcaat
      721 ggagcgtcga tgcgcgccat gaggaggtgg ggagccgttg ggtggggcgg cgcgtgcgtc
      781 ccgaggttgc cgggcccatc ctgttgcatg tcgcggcgaa gcgctatttg tgcgcgacgg
      841 atgctgacta tatccgcatg ctgagccccg cttccgtggt gacactcaag ctgcaggggg
      901 gaccgatgac gtcccctgaa atctcggcct tcgaagccga accggcgttc cggaacgccg
      961 tgcgcatccg gcactgggac gaccagggca aggtcgcggg tctgaggacg ccggggcttg
     1021 cagactatcg cgagctgatc gacagttttg cggttcggaa tacgtaaggt ccgtcgggcg
     1081 cacgagctcc gaggggcgta gtctgacaac ccggtcgggt ccatggagcc cgagggcaca
     1141 ggggggaagc ctgggatgag agcacagagc tggatcatcg gtgcgtgggc gatgctggca
     1201 acagtggcgg cgagctccgc cgtgccaccg ccgcgggcgg ccaatgctgt agtgcctgcg
     1261 gccagcgcgc cgcggttaca tctggccggc gtgcgcagcc tgcaacagcg cctcagtggg
     1321 agcaccgcca aactcgactc ggccctggcg gacctgtccg gccatctctc gcaggtgcgc
     1381 tccgatcacg cgctcgagga tctgtacgca ctcaatccgg ccgcgcgctt catgcagcgc
     1441 tccgcagccg aggggccgct cgtgctcgtg gatgccgtga cgaccggcga cccgcagaag
     1501 ctcaagagcg ctctggtgag cctcggcatg caacacgccg cagtgtattc gaacgatgtg
     1561 ggtggctttc tgccgctggc tcagctggcc aacgcggctg cgcgcgccga agtgcatttc
     1621 atgcgcgcct ccatgccgcg cacgcgcatc ggcgcggtca cttcgcaggg ggatttcgcg
     1681 cagcacagcg atgtcctgcg gagcacttac tccgcgctca atggcacggg tgtcacggtg
     1741 ggtgtgatgt cggacagcta tgactgttac gacgtgtatg ccgagccggg atccaaggtt
     1801 ccggtttccg gaccgacggg ctatgcgcca aatggattca cggcgtccgc aaccacggat
     1861 atttccaccg gcgatctgcc ttcgagtgtg gcgctcgtgg ccacctcgaa tgcgggcgag
     1921 tttccgtgtc tcagctacgg ggccccgacg ctgccgccct tctcggacga aggtcgcgcg
     1981 atgttgcaga tcgtgcatga cgtcgctccc ggagcctcac tggcctttca taccgccgag
     2041 tggagcgagg ccgatttcgc cagcggcgtg acggcgctgg cggccgcagg ggccaaggtc
     2101 atcgccgacg atgtggggta tttcgacgag cccttctatc aggacggcat catcgcgcag
     2161 gccatcgata cggtagaggg gaagggagtg gcgtacttct ctgctgccgg caacagcgcg
     2221 gatcacgcct gggagagcac gaacccgacc ttcgtggccc tgccgagccc gccggtaccg
     2281 ggtgagttgc tggtgaattt cgataccacg ggagcgacga aaaagaccac cctgtcggtg
     2341 gacatcccgg gcctgcagca gggagagctg ctggccgtcg tcgtcgaatg ggaccaaccg
     2401 tacgtgaccg gtgccccaca tagcgggggc gcgacgagcc acatcgactt gtgtatcagc
     2461 ggagccggtg cgaacaccat tatcaacacg agcggcgttg cagaaggctg caccggggcc
     2521 aatgcgaccg gatcggaccc ggtgcaggtg ctgattgtgg acaacccggc caatgcgccc
     2581 gcggtcacgg ctgccacgac actcaacatc accgtcggac tcctcgatgg cactgcacct
     2641 ccgggccgca tccgggtcgc ggtggatgac ggcggcgccg gcgccacgat cgatccaaca
     2701 gtagcgaccc acagctccac gatgcagggg catcccaacg cagccggtgc cgctgcgatc
     2761 ggcgcagcgg ctttcttttc gacgccggcg tgcggcacct cacccgcggt gctcgagaaa
     2821 tattccgcgt tggggagcgt gcagctgccg atcctgttcg atagcgcggg cacccggctg
     2881 gcgacgcccg tcctgcggca aaaaccggat ttcgtgggcc ccgacggagg cgacaacacc
     2941 ttcctcggct tcacgctggc gaaggccggg gcggattttc aggatcattc gacgatcgcg
     3001 cagtgcctcg acaacacgag ctatccgaac ttctttggga cctcggccgc gaccccgcat
     3061 gcagcggcca tcgccgcttt gatgctgcag gccaatcctg ccctcacacc aacgcagatt
     3121 tacacggcgc tgcgcacgag tgccttgccg atgtccggaa cgactccgga tccgagctcc
     3181 ggttccggat tcattcaggc cgatgcagcg ctgaagcttc ttccgcccgg tccgcccgcg
     3241 ctctcgttct ccgcatcctc cgtgatgacc ggaggcacca cgacgctcag ctggtcctcc
     3301 atcaatacct cgagctgcac ggcatcgggc agctggagcg gcgcacagaa aaccagcggt
     3361 agcgccacca tcacggcccc gtccacggcc ggggatgcgg cttacacctt gacctgcacc
     3421 aattccgagg gctcggccaa cgcgaccgtc catctcaccg taatggccgc gagctctcac
     3481 ggagggggcg gcggcgcagt ggacgagctc agcctgctcg ttctggcggc tctggggtgc
     3541 ctgcgactcc tgcggccggg aagggactca atcgtgtcgc cccggaggca tcgaggtccc
     3601 tgaccgaggc gtcctcggct gccgggggcg gcgctccctt gcgatcggcg tcggcgagga
     3661 actggccgac catcgcctgc ggttgcgcgg tgatgaacgg cgccttcagc gcacgcggca
     3721 cggcctcctt acggctcttg cgccacagat cgtagagggt tagcgtgccg gtgagggtcg
     3781 cgagcgtcgc atagaac
//
LOCUS       DASRHN010000095         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1351856, whole genome shotgun sequence.
ACCESSION   DASRHN010000095 DASRHN010000000
VERSION     DASRHN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351856"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VFB37_03535"
     CDS             complement(<1..1054)
                     /locus_tag="VFB37_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HZO21551.1"
                     /translation="MNAAPTTALLSELLLLLLIIAFSSGTEVAMLSVNRYRVKHRAQQ
                     GQGTARLLERLLQKPDDWLGANLVILAGASAFASAIATILAQRTGYTYAVPITVLILA
                     VVVIVFCELTPKIYAATHPESVALSAAYIYRALVLVTRPVLWLTNRMAYGLLWIVGVG
                     QTSRASQALSTEELRSVVVEAGPMIPARHRQMLLSILDLGQVTVNDIMIPRQEIAGID
                     VGENWDEILDQLRQTPHTRLPVYEGELDKLYGILHMKRVAQELARGTLTRDRLIEVAR
                     SREPYFVPESTPLTVQLSQFQRNRRRLAFVVNEYGDIEGLVTLEDILEEIVGEFTTDP
                     ATVVHKDIYIERPGVFI"
     gene            1225..2586
                     /gene="ffh"
                     /locus_tag="VFB37_03540"
     CDS             1225..2586
                     /gene="ffh"
                     /locus_tag="VFB37_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZO21552.1"
                     /translation="MFDNLTARLGRTIESLRGRGRITEENVGETLREVRVALLEADVA
                     LPVIKTFIDAVKAKALGVEVASSLTPGQAFIGILHRELVALMGGPRAGINLRAQPPIV
                     VLLAGLQGAGKTTTAAKLARWLIERERKRVLLVSTDVRRPAAMLQLERLCEQVGAQYF
                     AAQATDEPSGIARAAVEAARRGVFDVLIVDTAGRLHVDEALMAEVREIDTAVSPAERL
                     FVVDAMAGQDAVNSARAFGAALDLTGVILTKADGDARGGAALSVRQITGKPIVFVGVG
                     EKTEALEPFDPERMASRILGMGDVVSLVEQVHRQVDTQEAERLARKVVKGKGFDMMDL
                     RSQLEQLQKMGGVGALLDKLPTAAMSKKGAVSADQGDRDVRRQIAIINSMTPRERRHP
                     AIIDGSRRRRIANGSGVQVQDVNRLLKQFMEMQRVMKSMKGGKLRQLMSALKGGPPGF
                     PGR"
     gene            2792..3046
                     /gene="rpsP"
                     /locus_tag="VFB37_03545"
     CDS             2792..3046
                     /gene="rpsP"
                     /locus_tag="VFB37_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZO21553.1"
                     /translation="MVTIRLTRRGARNQPFYHVVVTDSRKRQGGPRLEHVGFFNPIAR
                     RSEPKLKLDLPRIDYWVSKGAQQSERVRSLVASYRKQATA"
     gene            3075..3581
                     /gene="rimM"
                     /locus_tag="VFB37_03550"
     CDS             3075..3581
                     /gene="rimM"
                     /locus_tag="VFB37_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZO21554.1"
                     /translation="MGWVELGRVGAPFGVKGWSHVDSYTDPPEGLLRYAQWELRLPSG
                     ERVTRRLAEGRLHGERLVARLEAVEDRDAAAALSGAVIEVSRAELPPAGKRQYYQADL
                     VGLPVRNLEGALLGTVAHFVEATAAPLMVVKDAAGREHWVLATPRHLHRVDLEAGLIV
                     VDWPVELE"
     gene            3598..4362
                     /gene="trmD"
                     /locus_tag="VFB37_03555"
     CDS             3598..4362
                     /gene="trmD"
                     /locus_tag="VFB37_03555"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZO21555.1"
                     /translation="MRIEVVTLFPQMISGALAYGVVGRAIERGLLAVGTEDPRRHAQD
                     VHRTVDDRPYGGGPGMVLKPQPLLAAIEAAHGRLCEGSPRIHLSAQGEPFDQRQAREL
                     AALPGFALVAGRYEGLDERIIELGIDRELSVGDYIVSGGELPALVVIDAVARLLPGTL
                     GDERSSEEESFAAGLLDWPHYTRPEVFRGLEVPQVLLSGNHADIQRWRLAQSVERTWR
                     RRPDLIAGHGLAPEASRLLNEFLAARAAGSDRGSRT"
     gene            4359..>4567
                     /locus_tag="VFB37_03560"
     CDS             4359..>4567
                     /locus_tag="VFB37_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZO21556.1"
                     /translation="MSSKIVQEIEKGRMSREVPDFKPGDTVVVQVKVIEGDRERVQAY
                     EGVVIAISNRGLNSSFTVRKISHGEG"
BASE COUNT          781 a         1374 c         1648 g          764 t
ORIGIN      
        1 cgatgaacac gccagggcgc tcgatgtaga tgtccttgtg cacgacagta gccggatcag
       61 tggtgaactc gcccacgatc tcctcgagga tgtcctcgag cgtgaccagt ccttcgatgt
      121 cgccatattc gttcacgacg aatgccagtc gccgcctgtt gcgttggaat tgcgagagct
      181 gcaccgtcag tggggtgctc tccggcacga agtacggctc gcggctgcgg gccacctcga
      241 tcagccgatc ccgtgtcagc gtgccgcgcg cgagctcctg agccacacgc ttcatgtgca
      301 ggatcccata gagcttgtcg agctcacctt cgtagaccgg caggcgggtg tgcggtgtct
      361 gccggagctg atcgaggatc tcgtcccagt tctcacccac atcgatgccg gcgatctcct
      421 ggcgcgggat catgatgtca ttcaccgtga cctgtccgag atcgaggatc gagagcagca
      481 tctgtcgatg ccgggccggg atcatgggac cggcttccac cacgacgctg cgcagctctt
      541 cggtgctcag agcctggctt gcccggctcg tctgcccgac tccgacgatc cacagcaggc
      601 cataggccat cctgttggtg agccagagca ccgggcgcgt caccagcacc agggcgcggt
      661 agatgtacgc ggcgctcagg gcaaccgact ccggatgggt cgcggcatag atcttgggcg
      721 tgagctcgca gaagacgatg accaccacgg ccaggatcag cacggtgatc ggaaccgcat
      781 acgtatagcc cgtgcgttgc gcgagaatgg tggcgatcgc cgaggcgaac gcactggccc
      841 ctgcgagaat cacgagattt gcacccagcc agtcgtcggg cttctgcagc agcctttcca
      901 ggagccgggc ggtgccctgg ccctgctggg cgcgatgttt cacgcggtaa cggttgacgg
      961 agagcatggc gacctccgta cccgaggaga acgcgatgat cagcagcagg aggagcagct
     1021 cactcagcag ggcggtcgtc ggggcagcat tcaaggtgca ttcctcgatc tgaagcggca
     1081 cggaggcaag ccggcagtgg tagtagcaca cgggatccgg gcgcgccaag ccggccgacc
     1141 ggccggggcg agagcgagcg gggtgctccg ggcagcccgc tataatcatc cccgggcgca
     1201 gcgagcgtcc ggcgcggaat catcatgttc gacaatctca cagcccggct cggccgtacg
     1261 atcgaatcgc tgcgcgggcg tggccggatc accgaggaga acgtgggcga gacgctgcgc
     1321 gaggtgcgcg tggcgctgct cgaggccgac gtcgcattac cggtcatcaa gaccttcatc
     1381 gatgcagtca aggcgaaggc gctcggcgtc gaggtggcct cgagcctcac ccccgggcag
     1441 gccttcatcg gcattcttca ccgcgagctg gtcgcgctca tgggcggacc gcgcgccgga
     1501 atcaacctca gggcgcagcc gccgatcgtc gtgctgctcg ccggtctgca gggcgcaggc
     1561 aaaaccacca cggccgcgaa acttgcccgc tggttgatag aacgcgagcg caaacgcgtg
     1621 ctgctcgtga gcacggatgt ccgccgcccg gccgcgatgc tgcagctcga gcggctctgc
     1681 gagcaggtcg gggcgcagta ttttgccgcg caggctaccg atgagccgtc ggggattgcg
     1741 cgggcggctg tggaggcggc ccgacggggc gtgttcgacg tgctcatcgt ggataccgcg
     1801 gggcgattgc acgtggacga ggccttgatg gccgaggtgc gcgagatcga caccgcggtg
     1861 agcccggcgg agcggctgtt cgtggtggat gcgatggccg gtcaggatgc cgtgaactcc
     1921 gcgcgcgcat tcggtgcagc gctcgatctg accggcgtca tcctgaccaa ggctgacggg
     1981 gatgcccgag gcggcgctgc cttgtcagtc cgtcagatca cgggaaagcc catcgttttc
     2041 gtcggcgtcg gtgaaaagac ggaggccctc gagccgttcg atccggagcg catggcatcc
     2101 cgcatcctcg gcatggggga tgtagtgagc ctcgtcgagc aggtgcatcg gcaggtggac
     2161 acccaggagg ccgagcggct tgcgcgcaag gtcgtcaagg ggaagggctt cgacatgatg
     2221 gatctgagga gtcaactcga gcagctgcag aagatgggtg gcgtgggcgc cctgctggac
     2281 aagctgccca ctgcggccat gtcgaagaag ggggccgtat ccgccgacca gggcgatcgc
     2341 gacgtgcgcc ggcagattgc catcatcaat tcgatgaccc cccgagagcg gcgccatccc
     2401 gccatcatcg atggctccag gcgccggcgg atcgcgaacg gctccggagt gcaggtgcag
     2461 gatgtgaacc ggctgctgaa gcagttcatg gagatgcagc gcgtcatgaa gagcatgaaa
     2521 ggcggcaagc ttcgccaact catgagtgcg ctgaaaggcg gcccgccagg gtttccaggg
     2581 cgctagccgg ggtgagtggc ctgaatggtg ggggccgcag gaatggcctg ctgctcttcg
     2641 cgggcaaaga ccacgttttt tgcccgcggg cgtgacctct tccgatggcc tggccggcct
     2701 ggataggtct ggcccatcca ggccaatacc agtagaatcg ccggtctttt gggccggatc
     2761 ggcccgaaat ctgcatgaga gcgatacgag tatggtgacg atacgcctga cgcggcgcgg
     2821 tgcgcgcaat cagcccttct accacgtggt ggtgaccgac agccgcaagc gccagggcgg
     2881 accccgcctc gagcacgtgg gcttcttcaa cccgattgcg cgccgcagcg agcccaaact
     2941 gaagctcgac ctgccacgca tcgattactg ggtgagcaag ggcgctcagc agtctgagcg
     3001 cgtgcgctcg ctggtggctt cctaccgcaa gcaggctact gcctgagggc agtcagcccg
     3061 ccggtggcgc caccgtgggc tgggtcgaac tcgggcgagt gggcgcacct ttcggtgtga
     3121 agggttggtc acacgtcgat tcgtacaccg atccgccgga gggcctgctg aggtatgcgc
     3181 agtgggaact gcgactgccg tccggggagc gggtcactcg gcgcctggcg gaaggtcggc
     3241 tccacggtga gcgactcgtc gcacggctgg aagcagtgga agaccgcgat gctgccgccg
     3301 ctttgagcgg tgcggtgatc gaagtgagca gggcggagtt gccgccggcc ggcaagaggc
     3361 agtactacca ggcggacctg gtggggctcc cagttcgcaa tctcgaaggg gcgctgctcg
     3421 gaacagtagc ccatttcgtg gaggcgacgg ctgcgccgct gatggttgtg aaagatgctg
     3481 cagggcggga gcactgggtg ctggccacgc cgcggcatct gcacagggtg gatctcgagg
     3541 cagggctgat cgtggtcgac tggccggtcg agctcgaata gcagcaggtg cggccatatg
     3601 cgcattgagg tcgtcacgtt gttcccgcag atgatcagcg gggcactggc gtacggcgtg
     3661 gtcggccggg cgatcgagcg gggcctgctg gccgtgggca ccgaggatcc acgccggcac
     3721 gcgcaggacg tgcaccgcac ggtggacgac cggccttatg ggggcggccc cggcatggtg
     3781 ctcaagccgc agcccctgct cgctgcgatc gaggcggcgc atgggcggct ctgcgagggt
     3841 agtccgcgga ttcatttgtc tgcgcagggt gagccgttcg atcagcggca ggcccgggaa
     3901 ctggcggctc tgcccgggtt tgcgctggtg gcgggccggt acgagggttt ggatgagcgg
     3961 atcatcgagc tcggcatcga tcgcgagctg tccgtgggcg actacatcgt ctcgggcggg
     4021 gagctgccgg cgctggtggt catcgatgcg gtggcgcggc tcctgccggg aactctcggg
     4081 gatgaacgct cgagcgagga ggagtccttc gccgccgggc tgctcgactg gccgcactac
     4141 acgcggcccg aggtgttcag aggcctggag gtgccgcagg tgttattgtc gggcaatcac
     4201 gccgacattc agcgctggcg cctggcgcag tccgtcgagc gcacctggcg gcggcggccg
     4261 gacctgatcg ccggacacgg actcgcgccg gaggcgagcc gtctgttgaa tgagtttctc
     4321 gccgcgcgtg cggcggggtc agatcgagga tcgagaacat gagcagcaag atcgtgcagg
     4381 aaatcgagaa aggtcgcatg agccgcgaag tacccgactt caagccgggc gacaccgtgg
     4441 ttgtgcaggt gaaggtcatt gaaggcgatc gcgagcgggt tcaggcgtac gagggcgtcg
     4501 tgattgcgat cagcaatcgc ggactgaact cctcgttcac cgtccgcaag atctcgcatg
     4561 gcgaggg
//
LOCUS       DASRHN010000096         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_32489, whole genome shotgun sequence.
ACCESSION   DASRHN010000096 DASRHN010000000
VERSION     DASRHN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32489"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFB37_03565"
     CDS             <1..744
                     /locus_tag="VFB37_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZO21557.1"
                     /translation="QVTHISGTPSHWRKALMSGFASRISPTYVRLSGEIADQAILDNL
                     RAIFPQASIAHAFASTEAGVAFSVEDGLTGFPPSLLNGSTRAGAEIRIEDDTLRVHSA
                     GSATRYLGHEGDLRDAQGFVNTGDRVELRDGRYYFMGRSGGIINVGGLKVHPEEVEAV
                     INSHPRVRMSLVKARRNPITGAVVTADVVLTGTEPTPSVAVSADETLKQEILDTCRGS
                     LAAHKVPAVIRIVPALEVSASGKLVRPNA"
     gene            737..1471
                     /locus_tag="VFB37_03570"
     CDS             737..1471
                     /locus_tag="VFB37_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO21558.1"
                     /translation="MRNVIVTGGSRGLGLSIAATLAASGYRVIAIARSETPALQEAAA
                     RLSGEKRGELRFRQFDLTDTSEISTLVAGLRKEHGPLYGLINNAGIGTAGVLSMMRDE
                     HIESLARLNIVSPIIMTKYVLRSMMVERAGRIVNISSIIGTTGYSGLSAYAATKASLL
                     GFTRSLAREVGQLGITVNAVAPGFVETDMTGELTQGERDQIIRRSALKRLAGVEDVAA
                     AVEFLLSEKAKNMTGVTLTVDAGNTA"
     gene            1613..2638
                     /locus_tag="VFB37_03575"
     CDS             1613..2638
                     /locus_tag="VFB37_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21559.1"
                     /translation="MNWWIGWPHAARDHGTFCVSCHTVLPYALARPALQRALGEAAPA
                     PAEVKLLENVSSRVQAWNQASPFYDDQHSGSDKTRQSRGTESVLNALILSSTGARAGR
                     VSPATQAALDIMWAQQRTDGPDRGAWDWLNFGLRPWEAADSRYYGATLAALAVGYAPA
                     VYRSDPAVRDHLQRLREYLDRESASQSLEHRLLLLWASMKLPGLLDQTQQQSIATEVL
                     DAQRSDGGWSLAPLARRHWRVFGLFPAQSDGYATGLATFVLEQISTPRARAAAAIGRA
                     WLRQNQQIPRGQWVARSLNKRRDLSTPVGLFMSDAATAYAVLALTTTPQSSASQTQAA
                     VSNALVR"
     gene            complement(2597..4366)
                     /locus_tag="VFB37_03580"
     CDS             complement(2597..4366)
                     /locus_tag="VFB37_03580"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HZO21560.1"
                     /translation="MNSPRKAWLRALEATAPIERLEAPSLPLQIAALADRFESAPALV
                     SAETQLTYRSLSAQVNRYARWARAQGLTAGDVVCLLMTNCPEYMAVWLGLTRAGITVS
                     LLNTNLTGEALAHGIRIVAARGVIVGSSLCDAIQTARAHLDPAIRYWQHGATNAGPPS
                     IDLEIARLSADPLPDSRWPAPALEDRALYIYTSGTTGLPKAATVSHLRILQWAYWFAG
                     MMNTGPNDRMYNCLPMYHSIGGIVATGALLVNGGTVVVRERFSAREFWKDIVKERCTL
                     FQYIGELCRYLLSSESQPEETRHVLRLCCGNGLRGDVWEQFQARFQIPQILEYYASTE
                     GSFSLYNCEGRPGAIGKIPPFLSHRVPVALVKFDPATEMPARDQSGFCTRCDADEVGE
                     ALGQIRDAAGHPGGRFEGYADPEASSRKLLRNVFETGDLWYRTGDLMRRDAQGFFYFV
                     DRVGDTFRWKGENVSTTEVASVISSCPGVLEVAVYGVSVPGTEGRAGMASLVVNGEFD
                     LVHLRDQLSQKLPPYARPVFIRLVSTIERTGTFRLRKAELAAQGFDPAQAGEIYMDDR
                     PSGGYVRLDASAYERIRNGGLRL"
BASE COUNT          821 a         1415 c         1440 g          805 t
ORIGIN      
        1 caggtgacgc atatctcggg caccccctcg cattggcgca aggctctgat gagcggcttc
       61 gcctcccgga tctcaccgac ctacgtgcga ctgtccggcg agatcgccga tcaggcgata
      121 ctggataatc tgcgcgcgat ctttccgcag gccagcatcg ctcacgcttt cgcttccacc
      181 gaggcgggcg ttgccttctc ggtcgaggat ggccttacgg gctttccgcc gagcctcctg
      241 aacggctcga cacgcgccgg cgccgagatc cggatcgaag acgacacgct gcgggtgcac
      301 tcggcaggat cagcgacccg ctatcttggc cacgaaggcg atctgcggga tgcgcaaggg
      361 ttcgtcaata cgggagaccg ggtggagttg cgcgacggcc ggtactactt catgggccgc
      421 agcggcggca tcatcaatgt gggcggactc aaggtgcatc ccgaggaagt cgaagcggtg
      481 atcaattcgc atcctcgggt gcgcatgtcg ctggtgaaag cacggcgcaa tccgatcacc
      541 ggtgccgtgg tcacggcgga cgtcgtgctc accggaaccg agccgacgcc cagcgtggct
      601 gtctctgcgg acgagacgct caagcaggag attctcgata cctgtcgcgg ctctctcgcg
      661 gcgcacaaag tgccggcggt gattcggatc gtgccggcgc tggaggtctc agcgtccggc
      721 aaattggtgc ggccgaatgc gtaatgtcat cgtcaccggt ggcagtcgcg gcctgggcct
      781 ctcgatcgcc gcaacgctgg ccgcatccgg ttaccgggtc atcgccatcg cgcgttccga
      841 aaccccagcc ctccaggagg ccgcggcgcg cctctcgggg gaaaagcgtg gcgagctgcg
      901 cttccggcag ttcgatctga ccgacaccag cgagatctcg acactggtcg cgggtttgcg
      961 caaggagcat ggtccgctct acgggctcat caacaatgcc gggattggga ccgcgggtgt
     1021 gctgtcgatg atgcgcgatg agcacattga gagtctcgca aggctcaaca tcgtctcgcc
     1081 gattatcatg accaaatacg tgctccgctc gatgatggtc gagcgggccg ggcgcatcgt
     1141 gaatatttca tcgatcatcg gtaccacggg atacagtggc ctctcggcct acgctgcgac
     1201 caaggcttcc ctgctaggct ttacgcgctc gctcgcgcgc gaggtcgggc aactcggaat
     1261 caccgtgaat gccgtggcac cgggctttgt cgaaaccgac atgaccggcg agctgacaca
     1321 aggcgagcgc gatcagatca tccgccgcag cgcgctcaag cgccttgctg gagttgagga
     1381 tgtcgccgca gcggtggaat ttctgttaag cgagaaggct aagaacatga cgggcgtgac
     1441 cctcactgtg gatgccggca atacggcctg agaatcctcc ggaacagccg tgcgtctgag
     1501 ctgcactctc ggcctcgctt gcgtggcagt attgtcagcg tgctcgccga gagtacctgt
     1561 cgatcaggaa agctgggacc cgaggaccgc tgctcagtac ctcgactcgc gaatgaactg
     1621 gtggataggg tggccgcatg ccgcgcggga tcatgggacc ttctgcgtct catgccacac
     1681 agtactgccg tatgcgctcg ctcggcccgc tctgcagagg gcacttggcg aggccgcccc
     1741 tgctcctgcc gaggtcaaac tgctggagaa tgtgagctcg cgggtgcagg cgtggaatca
     1801 agccagcccg ttctatgatg accaacattc cgggtcggac aagacgcggc agtcacgcgg
     1861 gaccgaatcc gttctcaatg cactgatcct gagctctacc ggtgcgcgcg ctgggcgagt
     1921 tagcccggcc acccaggccg cgctcgacat catgtgggcc cagcagcgca cagatggccc
     1981 ggatcggggg gcctgggatt ggctcaactt cggactgcgt ccctgggagg ctgcggacag
     2041 tcggtactat ggagccacgc tggctgcgct tgcggtcgga tacgcaccgg cggtgtatcg
     2101 gtcagacccg gccgtccgcg atcatcttca gaggctgcgt gagtatctcg atagagagtc
     2161 tgcatcgcag tcgctggaac accgactgct ccttctctgg gcctcgatga agcttcccgg
     2221 cctgctggac cagactcagc agcaatcaat tgcgaccgag gtgctggatg ctcagaggtc
     2281 ggatgggggc tggagccttg cacctctagc caggcgtcac tggcgcgtgt tcgggctttt
     2341 tcctgcgcaa agtgacggct atgccactgg actggcgacg ttcgtcctgg agcagatctc
     2401 gacaccccgc gcccgggcag ccgctgcgat cggacgtgcc tggttgcgtc agaatcaaca
     2461 gattcctcga ggtcagtggg tggcccgttc cctcaacaag cggcgtgacc tctcgacgcc
     2521 cgtcggattg ttcatgagcg acgcggccac ggcatacgcc gtactcgctc tcacaacgac
     2581 tcctcagtcc tccgcgtcac agacgcaggc cgccgtttcg aatgcgctcg tacgctgaag
     2641 catcgagtcg aacgtagcca cccgagggcc tgtcgtccat gtagatctcg ccggcttgcg
     2701 cgggatcgaa tccctgtgcc gccagctccg ccttgcggag gcggaacgtg cctgttcgct
     2761 cgatcgtgga caccagcctg atgaatacgg gacgggcgta gggcggcagc ttctgcgaga
     2821 gctggtcccg cagatgcacc aagtcgaact ccccgttgac gaccagcgag gccatgccgg
     2881 cccggccctc ggtcccggga acggatacgc catacaccgc cacttcgagc actccggggc
     2941 aggagctgat cacactggcg acctcggtgg tggagacgtt ctcgcccttc cagcggaacg
     3001 tgtcaccgac gcgatcgacg aaatagaaga atccctgcgc gtcgcgccgc atcagatcgc
     3061 cggtgcgata ccagagatcg ccggtctcga acacgttgcg cagaagcttg cgactggagg
     3121 cttccgggtc cgcatagccc tcgaacctgc cgccggggtg gcccgcggca tcgcgaatct
     3181 gcccgagcgc ctcgcccact tcatcagcgt cgcaccgagt acagaagccg gattgatccc
     3241 gagccggcat ctcggtcgct ggatcaaatt tcacgagtgc aaccggcacg cgatgcgaca
     3301 gaaacggcgg aatctttccg atggccccgg gacgtccctc acagttgtaa agcgagaagc
     3361 ttccctcggt cgaggcgtaa tactccagga tttgcggaat ctggaagcgg gcctggaact
     3421 gctcccagac atccccgcgc aaaccgttcc cgcaacacag ccgcaaaacg tggcgagtct
     3481 cttcgggctg gctctccgag ctcagcaggt aacggcagag ctcgccgatg tactgaaaga
     3541 gcgtgcagcg ctccttcacg atgtccttcc agaactcgcg cgcggagaag cgctcgcgca
     3601 ccacgaccgt gccgccattc acgaggaggg cgccggtggc cacgatcccc ccgatgctgt
     3661 gatacatcgg cagacagttg tacatccggt cgttcgggcc ggtattcatc attccggcga
     3721 accagtaagc ccactgcagg atgcgcaagt gactgaccgt agcagccttg ggaagtccgg
     3781 tggtgcccga ggtgtagatg tagagcgcgc gatcctccag tgccggggca ggccatctcg
     3841 agtccggtaa aggatcggcc gagagtcggg cgatctccag atcaatactt ggcggcccag
     3901 cattcgttgc accgtgctgc cagtaccgga tggccgggtc caggtgtgct cgcgcggtct
     3961 ggatcgcatc gcacagcgag cttccgacaa tgacaccccg cgcggcaacg atgcgaattc
     4021 catgcgccag cgcttcgccg gtcagattgg tgttgagcag ggagacggtg atgccggctc
     4081 gcgtcaggcc gagccagacg gccatgtact ccgggcagtt ggtcatgagc aggcaaacca
     4141 cgtcgcctgc agtgagaccc tgcgcgcgcg cccagcgggc atagcggttc acctgggcgg
     4201 agagcgagcg gtaagtgagc tgagtctctg ctgaaacgag tgcgggagcc gattcgaacc
     4261 gatctgccag cgcggcgatc tgcaggggca ggctcggcgc ctcgaggcgc tcgatgggcg
     4321 ccgtggcctc cagcgcacgc agccaggctt tgcggggtga attcacgttt tttccggtcg
     4381 aacgcggata atgggcccat gaggaaagag accatcgccc tgcacagtgg cttcgactgc
     4441 gatccgagca ccaaggccgt cgcggtgccg atctatcaga c
//
LOCUS       DASRHN010000097         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1021402, whole genome shotgun sequence.
ACCESSION   DASRHN010000097 DASRHN010000000
VERSION     DASRHN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021402"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFB37_03585"
     CDS             complement(<1..567)
                     /locus_tag="VFB37_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21561.1"
                     /translation="MSTVGRHQDATISYVRRGSLTYQARGKSTDLVPGSIIVAPPGVE
                     YVCSHNHAAWAECISFRFAAELVEQVAWQRPVWQIGCLPPVAELMVFGALAQSARDGG
                     VDLGLDEIGIALAARTAEIVSGTPARCNASRSGIDRRRAVDAALWIEAHSHEMVDLDV
                     LSRQVGLSPYYFLRMFSSVVGITPHQYLI"
     gene            complement(816..>3701)
                     /locus_tag="VFB37_03590"
     CDS             complement(816..>3701)
                     /locus_tag="VFB37_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21562.1"
                     /translation="GMTFRYRPVLAATVLALANAIAVHADPAPFDLAGPTLDVTVTRG
                     SQTLPISEVPNLAGGDHLSIKADFPATQSAHYLLIAAFLRGATNPPPKDWFFNCETWS
                     PKCAKGLSITVPQEAQQVLLFLAPETSGDFKTLVNAVRGRPGAFVRTSQDLNQATLDR
                     SRLEKYLTSVRALNGSDPAKLRQAAPLLARSLAIKVDDKCLDKIPELQAPCLMQGQAS
                     LILNDGHSTSIVEALTTGPASDLAMEASFTPQLSYGYYSPYIASVLDIAKIFDSFRTA
                     QYQYIPALGSPQGEHLALTLNAPPSFHNPKSVLVTALPAIEQQQLPPLHAVDPKEIYC
                     ARKSSLVLPVEGAPLVFSTGYAHDVTLSLTGKDGSSIDLPARADAEQGGFVVDTTALG
                     KATLGDSIHGSLHGLWGFEPYVGPSFQLMNAHAQSWEVDSQDDGKLIVGREGTIHLQS
                     ASVSCVDNIMLKDPAGKELTAEWKTVKPNEVEIKLPLQQAKPGALTLLVKQYGAGDPQ
                     AVPVHAFSEAGHLDSFAIHAGDSQGVLKGTRLDEVASLEIKGVQFLPGTLTTHQTADE
                     LTMIAQDAPAAVAWKQGDVVTAKATLKDGRVLSLSTLVATPRPSVRLLGKSVALSSSS
                     ADSNIQLNNEDELPQDAKLTFSVRSVQPAAFAYDQKIEVATVDEEFSTLLTLNNGVTL
                     ENSRVAVATLDPAKAFGPSAFGPLQFRVIANGVAGDWQRLATLVRLPLLKELKCPATS
                     ELACKLSGSNLFLVDSVSSDPKFSHPVQVPDGFPGYSMPVPHPTDGNLYVKLRDDPSV
                     INLTALGAQVLPPTSDEAARAPERHAAHSEPEPAPTPAPAPNGSASKTTVLSSSSNDS
                     SSAQPVPAAPPSAATSVSPTSPTPPATSVVASSAAPAPSGDVQAASHAPVQVPSTVNT
                     NKSAETPRGEATSSVPVPSAQPTATQPASAAQVPSGAPAVSGARPAG"
BASE COUNT          686 a         1164 c         1194 g          657 t
ORIGIN      
        1 tatcaggtat tgatgcggtg tgatgccgac cacggaggaa aacatgcgca ggaaatagta
       61 cgggctcagc ccgacctgtc gagacaacac gtccaggtct accatctcgt gggaatgggc
      121 ctcgatccaa agagccgcat caacggcgcg ccgtcggtcg atgccgctgc ggcttgcgtt
      181 gcagcgggcg ggagtgccgc tcacgatctc cgcggtcctg gcggccagcg cgataccgat
      241 ctcgtccaat cccaggtcca cgccgccatc acgtgccgac tgcgccagcg cgccgaacac
      301 catcaattcg gccaccggag gcaagcagcc aatttgccag acaggccgct gccaggcgac
      361 ctgctcaact aattcagctg cgaagcgaaa cgaaatgcac tcagcccagg cagcatggtt
      421 gtgcgaacag acgtactcca caccgggcgg cgccacgatg atggaacccg gcacgaggtc
      481 agtgcttttg ccgcgcgcct ggtaggtcaa actgccgcga cgaacgtagg aaatcgttgc
      541 gtcctggtgc cgtcccacgg tggacatatc gcaccggatc gccgtcaccg agagcgacgc
      601 ctgggacgtg agctccgtta taaatagccc aggggcaggt gtgtggactc cagccatagg
      661 tcacgcgatg caacatgcgt gcccatcgca ctcggcctgc gaccgatgtc tcgggcatta
      721 ccgccgcttg acgactaact ggctcgtcat aggaggcctt gtcttgagcg tgagctcaaa
      781 acgttgcgca gccagggtgt gtatagaatc cggcatcacc ccgcaggccg tgcgccggaa
      841 acggcaggcg cccccgaagg cacttgcgcg gctgaagccg gctgagtcgc tgtaggttgg
      901 gcggagggca ccgggaccga ggaagttgcc tcaccccgcg gcgtttctgc ggatttgttc
      961 gtattcaccg ttgacggcac ttgcacaggc gcatgcgatg cagcttgcac gtcgccggaa
     1021 ggcgcaggag cagccgacga cgcgaccacc gaagtcgccg gcggtgtagg cgatgtggga
     1081 ctcacggaag tcgccgcaga tggcggcgcc gcaggcaccg gctgtgccga gctgctgtca
     1141 ttggagctac tactcaacac cgtcgtcttc gaagcgctac cgttaggcgc cggcgcggga
     1201 gttggcgcag gctccggctc ggagtgcgcc gcgtgccgct ccggcgcgcg cgccgcctcg
     1261 tccgaggtcg gcggcagcac ctgcgcgccg agcgctgtca agttgatcac cgacggatca
     1321 tcacgcaact tcacgtagag attgccatcg gttggatgcg gcacgggcat ggagtatccc
     1381 ggaaaaccgt ccggcacctg cacgggatgc gagaacttcg gatcgctgga cacggagtcc
     1441 accaggaaga gattcgaccc ggagagcttg caggccagct ctgaggtcgc gggacacttc
     1501 agctccttca agagcggcag gcgcaccagc gtggccagcc gctgccagtc acccgcaacc
     1561 ccgttggcga tcacccggaa ttgcaaggga ccgaaagccg acggcccaaa cgccttcgcc
     1621 ggatcgagtg tcgccaccgc gacccgggag ttctccagcg tcacaccgtt gttgagcgtg
     1681 agcagcgtcg agaactcctc gtcgacggtt gcaacttcga tcttctggtc gtaggcgaac
     1741 gcggcgggtt gcacgctgcg aaccgagaac gtgagcttcg catcctgcgg caactcatcc
     1801 tcattgttga gttggatgtt gctgtcggcg ctcgacgaag acagcgccac actcttgccc
     1861 agcaatctca cactgggacg gggcgtggcc accagcgtgc tcaaggagag cacgcggccg
     1921 tccttgaggg tggccttcgc ggtcaccacg tctccttgct tccaggccac ggccgccggc
     1981 gcgtcctgcg cgatcatggt caactcgtcg gcagtctggt gcgtcgtcag cgtgccgggc
     2041 aggaactgca cgcccttgat ctcgaggctg gcgacctcgt cgaggcgggt gcctttgagc
     2101 acgccttggc tatcaccggc atgaatcgca aagctatcga gatgtcctgc ttcggaaaac
     2161 gcgtgcaccg gcaccgcttg cggatcaccc gcaccgtact gcttcaccaa caacgtgagc
     2221 gcacccggct tggcttgctg cagaggcagt ttgatttcca cctcgttcgg cttgaccgtc
     2281 ttccattcgg cggtgagctc cttgcctgcc ggatccttga gcatgatgtt gtccacgcag
     2341 ctcacgctcg cggactgcag atggatcgtc ccctcgcgac cgacgatgag cttgccgtca
     2401 tcttgggaat caacttccca gctttgcgcg tgcgcgttca tgagctggaa gctggggccc
     2461 acgtacggct cgaagcccca gagtccgtgc agcgagccgt gaatactatc cccgagcgtc
     2521 gcttttccca gagcggtcgt gtcgaccacg aagccaccct gctccgcatc cgcgcgtgcc
     2581 ggcagatcga tcgaggagcc gtccttgccg gtcaggctca atgtcacgtc atgcgcatac
     2641 ccggtggaga acaccagtgg cgccccttcc acgggcagca ccagtgagct cttgcgagcg
     2701 cagtagatct ccttgggatc caccgcatgc aggggcggta actgctgctg ctcgatcgcg
     2761 ggcagggccg tgacgagcac cgacttcgga ttgtgaaacg aaggcggagc gttgagcgtc
     2821 agcgcgagat gctcgccttg aggcgagccc aaagcgggaa tgtactggta ctgcgcggtg
     2881 cggaaggaat cgaagatttt cgcgatgtcc agcaccgacg cgatgtaggg gctgtagtac
     2941 ccataactca actggggcgt gaaactcgcc tccattgcca agtcgctggc gggccccgtc
     3001 gtgagcgctt cgacaatgga cgtgctgtgc ccgtcattga gaatgaggga agcctgtccc
     3061 tgcatcaggc agggcgcctg cagctcaggg atcttgtcga ggcacttgtc gtccaccttg
     3121 atggccagac tccgggccag caacggcgcc gcttggcgca atttcgccgg atcggacccg
     3181 ttcagggcgc gcacggaagt cagatacttc tccaggcgcg agcgatccag cgtggcctgg
     3241 ttcagatcct gcgaagtgcg cacaaaggcg ccggggcggc cgcgtaccgc attcaccaga
     3301 gtcttgaagt cgccgctggt ttccggcgcg agaaagagca gcacttgctg cgcttcctgc
     3361 ggcaccgtaa tgctcagacc cttggcgcac ttggggctcc aggtctcgca attgaagaac
     3421 cagtccttcg gtggcggatt ggtggccccg cgcaagaacg cggcgatgag caggtagtgc
     3481 gccgactggg tggcgggaaa gtcggccttg atcgagaggt gatcgccccc cgcgagattc
     3541 ggtacttcgg atatgggcag cgtctggctg ccccgcgtga ccgtgacatc gagtgtcggg
     3601 ccagccaggt cgaagggtgc agggtcagcg tggaccgcaa tggcattcgc gagcgctaag
     3661 accgtggccg cgagcacggg gcgatatcgg aaagtcatgc c
//
LOCUS       DASRHN010000098        42885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1847731, whole genome shotgun sequence.
ACCESSION   DASRHN010000098 DASRHN010000000
VERSION     DASRHN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 42885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42885
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847731"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..592
                     /locus_tag="VFB37_03595"
     CDS             95..592
                     /locus_tag="VFB37_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21563.1"
                     /translation="MRRRPHATLGGILVGGWIAGTLDIGAAALINHISPLIILRFIAA
                     GVLGLQSLKMGLTSSALGLLLQWAMSILIAAICILVTQPIAGMRRHWIPLGLAYGVAT
                     FFIMNYVVVPLSAIGHGPKFTPEHFAANLLAMLVFGLIIAFFTRAAGGHFALGPLPGR
                     PPARS"
     gene            688..1647
                     /locus_tag="VFB37_03600"
     CDS             688..1647
                     /locus_tag="VFB37_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZO21564.1"
                     /translation="MLLLVDGRHLAAMKRLSPDEDAAHRVLEITRAWIKEAVTELRKS
                     TLDDEVVHSARKALKKARAGLRMLRPGLRDAEYRNANAALRDASRPLGELRDAKVLQD
                     TLTDLMRRRRSRRRNGLNVARVSSLLKEHRRRVRRQILHDKRALAHSRHLLRVTLSRT
                     KKWRLRKTNWKLLGPALEHAYRKARKARADAWTIGTPEALHEWRKQVKYLRYALEIAE
                     PLWPAMLGVLAKQAHELSDELGDSHDLTALCEMTVAHPDAFANSSELDALLALSEHRQ
                     QELTGEANLLGARLLGERPPAFGARLEGYWRNWHQGAGLVHAH"
     gene            1747..2211
                     /locus_tag="VFB37_03605"
     CDS             1747..2211
                     /locus_tag="VFB37_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21565.1"
                     /translation="MSAVLTALYSNHETAEQVRTRLVQDGFPTDRVQLTSRQELGQAD
                     VAPAKELPEKLKQYFHQLFQNERAQRSAQRLERAVLDGGAVIAVHPRGDVETQRALAL
                     MEDSGPVEFRASDLQNQAGEHAASSGSEALSWIGKIMVAPLAPHRTSPGAGG"
     gene            complement(2256..3707)
                     /locus_tag="VFB37_03610"
     CDS             complement(2256..3707)
                     /locus_tag="VFB37_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO21566.1"
                     /translation="MNRIVRSSAFTLAFSYVAFGIGALILFAAPLWYAWQVTIQDSRT
                     ELLRVDTQRLAEVFAREGPSGLKAFINTRVGMQIPGERILLLTDPSLHPVAGNLPEWP
                     RVIPVEPGTYTLPVNLAGHHTKAMFVHTVLPGGYNLLVGRDQTLFAPLTTRFWYGLAA
                     AVAVLSIVGVLGGLMVRRALLLRVDNIRHTVLAIVQGDWSHRLPTKPSSDELDTLSRT
                     INGMLDQIEQLLHGVRDVSNSIAHDLRTPLAELRSRLEELSLTRPEAEETFAEIDAAV
                     VDVDRVIRIFNALLRLAEIDAGMRRSGFVAINAADIARQAVDFYLPAAELKGISLSYR
                     GGPAGISGDPVLLAQAIGNLIDNALKYAGEGGQIEVEVQTLADGRVRISVADGGPGIP
                     DSEKPKVPTRFYRGDTSRGTPGVGLGLSLVASVAKVHGGALELADNHPGLRANMLIAP
                     GTVWASGAREGQPAEAPSKESSAELHPESVEAS"
     gene            complement(3700..4380)
                     /locus_tag="VFB37_03615"
     CDS             complement(3700..4380)
                     /locus_tag="VFB37_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21567.1"
                     /translation="MKVLLVEDNERVRRFVVKGLQEAGHTADHADNGRDGMFLAASER
                     YDVIVMDRMLPGDIDGLAIIEALRKLGNRTPILILSALSDVDERIRGLKSGGDDYLTK
                     PFAFGELLARLDALCRRVQGGGTQTSLKVGDLHMDLLSRKAARGSRAIALQPQEFKLL
                     EYLMRHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISKLRQKIESDSERPLLRTVRNA
                     GYMLAADE"
     gene            complement(4410..7535)
                     /locus_tag="VFB37_03620"
     CDS             complement(4410..7535)
                     /locus_tag="VFB37_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO21568.1"
                     /translation="MSISEPFIRRPIATSLLMAGILLIGIVAYPLLPVAPLPQVDFPT
                     IQVSANLPGASPETMASAVATPLEYQFAEIPGVSQLTSSSVLGNTQITLQFDLGRNID
                     AAAQDVQSAIDAAGGQLPKNLPSPPSYRKVNPADSPILIFSVHSDVLPLTVVDDYAEN
                     VLTQQISRIPGVAQVSLGGQRKPAIRVQVDPAKIAALGIQLEDIASVISEATVDAPKG
                     AINGVHHSFAIYDNDQLLKAAPWNDVIVAYKNGAPVRIRDIGVAVDGPENELSFAFQK
                     GGDGVLLMVFKQAGANVIDTVKRVNALLPHAMASVPPSIKVSTIIDRTTTITASVHDV
                     EYTLLLTIVLVVLVIFLFLRNVWATIIPGVTVPLALLGTAALMYVLGYTLDNLSLMAL
                     TIAVGFVVDDAIVMLENIYRHLESGLSPLQASLKGSREIGFTIMSISISLVAVFIPLL
                     LMGGIIGRLFREFAITVTMTIAVSAFVALTLSPMMCSLFLRDEHRVSHGRLYLTIEKG
                     FDKVLRGYTRGLDFVLTHQRPTLVVFLLTVVLSGLLYVFVPKGFFPQQDTGILMGLSD
                     ASQDVSYDEMMKLQKQLMEVVNEDPAVASWATHIGGSHPVNSGWVGIGLKPRSERTAT
                     ADQVITRLRAKLANVKGATLFLQASQDLGVGGRTTRTQYQYTLQDSNLDELNEWAPKM
                     LAQMQKLPALRDVASDQQTNGTTLSLVIDRDQAARFGIQPALIDQTLYDAFGQRQAAQ
                     YFTQLNAYHVILEITPQLQKDPHSLDKIYIKSPITNQQVPLSTFVHYDTQHVSYLSVN
                     HQGQFPAVTLSFNLAPGAALGDAVDGIHRIATTMGMPASMLGTFQGTAQAFESSLGSE
                     PYLILAALVVVYIILGMLYESYIHPLTILSTLPSAGVGALLMLLAFHFDLSVIALIGI
                     ILLIGIVKKNGIMMVDFAIQAEREQHLSPEAAIRQACLLRFRPIMMTTMAALLASLPL
                     MLGTGTGSELRQPLGYTMVGGLLLSQALTLFTTPVVYLYLDRVNARLSRHRLGADADR
                     PDDTHHEPATV"
     gene            complement(7532..8779)
                     /locus_tag="VFB37_03625"
     CDS             complement(7532..8779)
                     /locus_tag="VFB37_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO21569.1"
                     /translation="MLTRHRRRVWLLGAGALVLLIVGWHGLPGTRTEAASPPRDPNQT
                     VAVDATAVKREDVPVYLDGLGTVQAFNTVTVTARVDGELQKIGFVEGQMVKVGDLLAQ
                     IDPRPFQAVLAQAQAAKAKDQAQLQSARADLERYTVLAPENLASKQTLDSQRALVAGL
                     EAQIKGDQASIENAQTQLQYTTITSPIQGRTGIRRVDAGNNVHATDTNGIVVVTQMQP
                     IALIFTLPEETLDSVGSALSAGKVQVVALSRDGGTELDRGTVTLIDNQIDQSTGTIRL
                     KAVFPNIQNRLWPGQYLTARVLVRTEHAALTIPSNAVERGPNGMYTYVIKPDSTVEAR
                     PLKVAEESGAVMVVRDGLHEGERVVTSNQFRLQPGARVQISSNSSLGGDSGPEVATVP
                     DRSPPMAATPPPRKRSTLGRTVQ"
     gene            complement(8851..10404)
                     /locus_tag="VFB37_03630"
     CDS             complement(8851..10404)
                     /locus_tag="VFB37_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO21570.1"
                     /translation="MIRADSSSFAALRRRSSWCRGAAALGVAVLLSACSLGPRYKRPE
                     IPPPAAWRTDITETPTEAAAEKTAAQQPAGSSTGASASAKLGTTPAWPSADWWRGFGS
                     TQLNDLIAQAQRANDDIAAAIARVRQADAQAQIAGAPLFPTLDVSGDASRQREKASGA
                     LPGAPPITFSEFSALASASYELDFWGKNRATFNAAKFAAQASRYDRATIELSVMTSVA
                     NTYFQAIELHDRLNVAQQDLSAAQTVLKDLQLEEQVGTAAALDVAQQETTVAVLNAAI
                     PPLRQQLRQTIDALAVLVGRMPQELDAPESTLTDLSEPVVGAGLPSELLARRPDVAEA
                     EAQLMSANANIAAARAAFFPSIDLTADGGFVSTALSSLVTPLNRIFSLSAGVTQQIFA
                     GGALVGNYRLNKGRYAELLADYHKAVISAFSNVEDALVAAQQTAEQYRRQQEAVAKAR
                     RAYEITEIQLHAGTVNVLTVLNTQSALFTAEDELVQARFAHFQTLVQLFSALGGGWQQ
                     GQSPQTGHG"
     gene            complement(10540..11148)
                     /locus_tag="VFB37_03635"
     CDS             complement(10540..11148)
                     /locus_tag="VFB37_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZO21571.1"
                     /translation="MELVPMVLEQSSRGERSFDIFSRLLRERIIFLNGPINDGVAALV
                     CAQLLFLESENPSKDIALYINSPGGVVTSGFAIYDTMQWVKCPIWTVCIGMAGSMGSF
                     LLMAGARAQRIALPNATILLHQPSGGFQGQASDVERHAEDIVKTKRRMTQLYATHCGR
                     TYEEVEKVLDRDRFMSAEEARDWGLVDHVYKTRDIPGLVNGP"
     gene            complement(11148..11636)
                     /locus_tag="VFB37_03640"
     CDS             complement(11148..11636)
                     /locus_tag="VFB37_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZO21572.1"
                     /translation="MRELTRQPLQQGTTGNKREASRDAVVSECELGAAPERVWRALTE
                     PELLAKWLLPNDIRPLRGARFTLQGPPGTPDGRVECEILEAQPNRLLQYRWRIAADGS
                     EAAAGPPLDSTVTFELFPTITGGTLLRVIHAGFAAAIPHSIASQTRASTACASAGLRW
                     AA"
     gene            complement(11633..11947)
                     /locus_tag="VFB37_03645"
     CDS             complement(11633..11947)
                     /locus_tag="VFB37_03645"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO21573.1"
                     /translation="MLEAQVFRTLADPTRRAMFEALASGERSVSELTRRLSISQPAVS
                     QHLGALRRVGLVRERRAGRFTLYQAEPAGLSPLIGWIDRYRAFWPERLQRLRTVLEDF
                     EP"
     gene            12127..12504
                     /locus_tag="VFB37_03650"
     CDS             12127..12504
                     /locus_tag="VFB37_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21574.1"
                     /translation="MSTTADHFQAALLALTRSGSIKDRLLEAYRNHLAQVEESELPAE
                     VREEFRLFSQALTREPPLLRGEDASRATVRKMSLHEAAAMASIVVRMFAAVSGGAALG
                     SWSKSMRSDSNVVPLYQAEANRA"
     gene            complement(12491..12943)
                     /locus_tag="VFB37_03655"
     CDS             complement(12491..12943)
                     /locus_tag="VFB37_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21575.1"
                     /translation="MSQANPIRLFVTHAWENSDDYLRVFEYLESSRNFFYRNSSKPEL
                     RPQGDKEALKEELRRQIAPAEVVIGLSSLHETSQDLVTFQLLYAQASQKPVVLMKPFG
                     SQRPVPKALCDLADELIDWDERALVDAIRRQARHEETTRWDTIEFKLD"
     gene            13110..13874
                     /locus_tag="VFB37_03660"
     CDS             13110..13874
                     /locus_tag="VFB37_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZO21576.1"
                     /translation="MRLLVYNIRYATGTGPAFYLPIPGAGYLRSNRKVLEGITNFIRA
                     EHPDVVGLIEVDTGSIRTGMLNQAEHIAGSIGHYSTFQCKYGTGSINHLVPIVRKQAN
                     AFLSAPHVHGERFHYFDTGIKRLIIELELDDVCVFLVHLSLKFRHRQYQLRSLHDLIV
                     QSPKPVIVAGDFNTFWGTHEIYLFMRAAGLRSANSAGLPSFPARVPRIELDFILVSEG
                     IEVTDFRVPDVRFSDHRPLVCDFHVRTDALVRRTAA"
     gene            13887..15932
                     /locus_tag="VFB37_03665"
     CDS             13887..15932
                     /locus_tag="VFB37_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZO21577.1"
                     /translation="MNDSPAWSSERDADGVLWLSIDKPGTSANVLSSNVLRELDALLQ
                     PLAQQPPRAVVVLSAKKSGFVAGADIKEFTGISNAEEGYQLIRAGQQVLDHLEALRCP
                     TVAAIHGFALGGGLELALACRYRVAVADERLSLGLPEVQLGIHPGFGGTVRSVRLVGV
                     KAAMELMLTGKPVRADKALRIGLVDKLVPAAELRAAAKAMAMQPPPLHRAPFAERLLS
                     WGAMRPFVKNALLAQVRSKARREHYPAPYAMIDLWARWGAQGHAAFEAEARSIAQLFL
                     SETSRNLVRVFLLQDRLKSLGGKVAQEQKHVHVVGAGVMGGDIAAWSALRGFTVTLQD
                     RSLELIEPALKRAQELFEKRLREPGAHSAARERLRADVAGDGVPSADVVIEAIFENLE
                     AKRELYARLEPRMKPAALLATNTSSLMLEPLAANLARPGQLVGLHFFNPVAQMPLIEI
                     VQAQQTDATVMQAATAFARRLDKLPVPCRSAPGFVVNRVLMPYLHEAMFAAQEGVPLS
                     VIDATAVSFGMPMGPIELADVVGLDVASHVGEIIAAELGRPAPDLSRLKELIAAKKLG
                     RKSGEGFYTWHEGKPRKPTPPDTTAPPDLIDRLILVLVNECVACLRERIVAEADLIDA
                     AVIFGTGFAPFRGGPLAYARSRGVGAVVARLEELAVRHGPRFRPDSGWPQLSVALKS"
     gene            16080..17150
                     /locus_tag="VFB37_03670"
     CDS             16080..17150
                     /locus_tag="VFB37_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZO21578.1"
                     /translation="MPEEREASVQLLKTFAPLDGLKRDNLAALAKKVSVRTMPAGRML
                     FKEGDTDKRTVWLVAGMVEIREGERTIAMIRGGTPDARNPLYPKIPRTVSARAVDEIS
                     YLSIDSELLDVMITWDQTGTYEVAELQTRLDGTSSDDWMTTLLQTKAFHRIPPANIQA
                     IFMRLQRFPCRAGDIVIKQGDEGDYFYVIVNGKCVVTRETPLNREGIKLAELGVGDTF
                     GEEALIAEAKRNATVTMLTDGVLMRLNKQDFRELMNEPLLQWVPYEKATEIIARGGRW
                     LDVRLPSEHQNLAIEGSLNIPLYFIRLKISALDRNIPYVVYCDTGRRSSAAAFILVER
                     GFDAYVLTGGLTNSNIALRRSA"
     gene            17523..17783
                     /locus_tag="VFB37_03675"
     CDS             17523..17783
                     /locus_tag="VFB37_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21579.1"
                     /translation="MSELKISDEGGRSTGWTWLIVLAMVTLPVLAWFFFIPVAAVALV
                     AACIIGTVGGAARRARAQVREQRKQQMQSAEVALNDYWRPMS"
     gene            complement(17863..18213)
                     /locus_tag="VFB37_03680"
     CDS             complement(17863..18213)
                     /locus_tag="VFB37_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZO21580.1"
                     /translation="MRTGGNKPGLLTLTIKDKSALYLAYMPFVRNGGLFIPTNSNYRL
                     GDEVFMLLNLMGEDEKLPVAGRVIWVTPKGAQGKRTAGIGVQFSDQDRGQTQKKIENY
                     LAGALSGDKATHTM"
     gene            complement(18218..19204)
                     /gene="holB"
                     /locus_tag="VFB37_03685"
     CDS             complement(18218..19204)
                     /gene="holB"
                     /locus_tag="VFB37_03685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZO21581.1"
                     /translation="MSMSWLRNQMAALQAAYAADRMPHALLIHEAPGAGGEWLAGWVA
                     QLVLCAHPERAPCLACGACQRVRARHHPDLISLEPVEDSRQIRIEQVRELAQELALTS
                     HQGGYKLAIVSPADLLNRFAANALLKTLEEPPARTVLILVATQPSRLPATLLSRCQRI
                     QVPAPARAEAIGWLEATRGKADWNAVLDVLGEAPWHAAAADPAVIVQISGEVQRSLDE
                     AVRGALDPAATAERWARTELPLRLRCFENWLTKRIQEQSGTTGGFTEVGAVPYLQGAG
                     TVLNIRALFGLLDGVRDLRSALETPINRGLALEDLLRHLAPKIENTAVSRAR"
     gene            complement(19201..19857)
                     /gene="tmk"
                     /locus_tag="VFB37_03690"
     CDS             complement(19201..19857)
                     /gene="tmk"
                     /locus_tag="VFB37_03690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZO21582.1"
                     /translation="MIRGRFITLEGIEGAGKSTVAVAIAEWLVRQKLRARLTREPGGT
                     PLAERVRTIVLERGTEHVSAVTETLLMFAARAIHLDNLIRPALGHGEWVICDRFTDAT
                     RAYQGYGRGMNLAWIEQLASAVHADLQPERTLILDLPVEVGLARARTRSGAAADRFEA
                     EQIEFFERVRAGYLAIAGGAPERIRVVDAARPVNEVIEQVIAALAGLMNGSPEDDLPR
                     "
     gene            complement(19854..21002)
                     /gene="mltG"
                     /locus_tag="VFB37_03695"
     CDS             complement(19854..21002)
                     /gene="mltG"
                     /locus_tag="VFB37_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZO21583.1"
                     /translation="MPSSRGRVLVVLLALILLTVCAAGAGYLWLQRLFHSPGPSQSIA
                     RIQVEQGASVRTVLSELGNLGALRHPRAIEVYLRLSGRLSGHRLRIQAGMYEIPAAAS
                     PAQILDLFDQGKVVLEQITIIEGTTFADFRRALDHHAAVTHTLLGKSSAELMAALGHP
                     GEAPEGLFFPDTYRFAARTADLEILSLAYNAMQRVLGSAWQQRSVDLPLQSPYEALTL
                     ASIIEKETGTPEERARIAGVFVSRLRKGMRLQTDPTVIYGLGASYEGEIRTKDLLKDT
                     PYNTYTRAGLPPTPICLPGRDSVLAAVHPQETGELYFVATGTGDGRHHFSKTLEEHNK
                     AVKSYLEHLRALQRAGVTHGATGTAPHAETATGSHEPAGPDRTPPGTK"
     gene            complement(20995..21888)
                     /gene="pabC"
                     /locus_tag="VFB37_03700"
     CDS             complement(20995..21888)
                     /gene="pabC"
                     /locus_tag="VFB37_03700"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:NF004761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HZO21584.1"
                     /translation="MIRKQPARYDPGSVLPGAGSERMLVDGTEAAGVSTLDRGLHFGD
                     GLFETIACRAGRPRFLSLHLERLMSGCKRLQIAFHDEAAVRSEVEELAHGSDRALVKV
                     LVTRGSAVARGYGFTGREKATRITIRYAWPAEDSAPPHAGLKAGTLALRLGENPLLAG
                     LKHCNRLEQVLARAECLNRGLSEGILFSSSGRLVSGTMTNVFLVRGSTLQTPLLDVCG
                     VAGVMRRIVLRQAASAGIEAHECALDLADLRAADEVFLTNARVGIWPVRELDEQVLAP
                     GALTHRLLEELTPLLEEPRDA"
     gene            complement(21885..23276)
                     /locus_tag="VFB37_03705"
     CDS             complement(21885..23276)
                     /locus_tag="VFB37_03705"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF006563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZO21585.1"
                     /translation="MGARNPPVIRDLDVAPSVLRRMAARFPDRYPFLFDSAADGALSH
                     SSILVAAPGECLWLDREGRLGADGVAPAGSGFLQALENWWLTERRPATPEYPAFCGGW
                     SVFLSYELAAEVEPHLRLPAASLPWRAFAVRTRAALMHERASGRVYMIAEPEAAQALE
                     RIESDARSTAALAAPDPQAIRVVSLEEEVPEAYLERVVRAKEYVKAGDIYQANLSRRW
                     RLQLSGEPDVGRLYERLCSANPAPFAALVQWRGARILSSSPERLVRIHGRRIDTRPIA
                     GTRPRSRRPGDDHAEMRELAAHPKERAEHIMLIDLERNDLGRVCRAGTVRVDELMSIE
                     SYAHVHHIVSNVSGELRPEVTPIGALRAVFPGGTITGCPKFRCMQIIAELEAEGRGAY
                     TGSLGYLGRDGLMDMNILIRTMTVMGRAVELRAGGGIVADSDPQRELEETRAKARGML
                     AAFNLNALGRADT"
     gene            complement(23311..24558)
                     /gene="fabF"
                     /locus_tag="VFB37_03710"
     CDS             complement(23311..24558)
                     /gene="fabF"
                     /locus_tag="VFB37_03710"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012149092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZO21586.1"
                     /translation="MSKRRVVITGLGIISPVGSTLKSAWEAILRGESGIGPITRFDVS
                     AFPVRFGGPVRDFDVSQYIAPKDARRMDDFMHYGVAAGIQAVTDSGIDFSKLDSARCG
                     AVCGAGIGGLGTIESEYDNYLKGHSPRKISPFFVPATIINMIAGHLSIKYGLRGPNLG
                     VVTACTTSTHAIGLAMRTIQYGDADVLIAGGGEMATTVCGLGSFGQAKALSTRNDAPK
                     QASRPWDRDRDGFVLSDGGGAVIMEELEHARARGANIYAEIIGFGMSGDAYHITAPPE
                     DGEGARLAMSNALADASLNAADVQYINAHATSTQLGDKAETTAMKRAFGEHAKRLAVS
                     STKSMTGHLLGAAGVIEAIFSILAIRDGVVPPTINYHTPDPDCDLDYVPNTARRMKVD
                     VALSNSFGFGGTNGSVIFRRFTG"
     gene            complement(24655..24897)
                     /gene="acpP"
                     /locus_tag="VFB37_03715"
     CDS             complement(24655..24897)
                     /gene="acpP"
                     /locus_tag="VFB37_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZO21587.1"
                     /translation="MSTVEQQVKAIVAEQLGVKQEQVTNDASFVDDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKITTVQQAIEYINDRRTKA"
     gene            complement(25125..25886)
                     /gene="fabG"
                     /locus_tag="VFB37_03720"
     CDS             complement(25125..25886)
                     /gene="fabG"
                     /locus_tag="VFB37_03720"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZO21588.1"
                     /translation="MTTQEAGRLENEVALVTGASRGIGHAIALALAEAGAQVVGTSTS
                     AEGAAAFGATLASHGYNGRGAVLDVGDAASIEALIQELDAAGQMPTILVNNAAITRDM
                     LLLRMKPEDWERVIATNLTSVFRLSKACLRRMMKERRGRIINLTSVVGVTGNPGQANY
                     AAAKAGILGFTKSLAREVATRGITINAVAPGFIDTDMTRALNEEQRAALLTQIPMGRL
                     GTPADVAAVVLFLSSARAAYVTGQTLHVNGGMYMP"
     gene            complement(25883..26863)
                     /gene="fabD"
                     /locus_tag="VFB37_03725"
     CDS             complement(25883..26863)
                     /gene="fabD"
                     /locus_tag="VFB37_03725"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZO21589.1"
                     /translation="MSFAFVFPGQGSQSVGMLAGYAEVPVVRRTFDEASGVLGYDLWK
                     LVEEGPREALDATERTQPAVLAAGMAVWRLWRERGGGEPALVSGHSLGEFTALTCAEA
                     LDLPAAIGLVRFRGQAMQEAIPAGTGAMAAILGLEDAQIEAACREAAQGAVVDAVNFN
                     SPGQVVIAGERGAVQRAIELMKARGAKRAIELPVSVPSHCNLMRAAGERLGEYLARVD
                     LRPPRLRYFSPVDVAFHEQPEEIRAHLARQLLRPVQWTRTIRAIASQVAQIIECGPGK
                     VLTGLNRRIEKRPGLDFRALDDLESIGAALQATQAAAAGGERGPGGEQRA"
     gene            complement(26929..27546)
                     /gene="rrtA"
                     /locus_tag="VFB37_03730"
     CDS             complement(26929..27546)
                     /gene="rrtA"
                     /locus_tag="VFB37_03730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HZO21590.1"
                     /translation="MGKVGPVKAVRRLLTSLNCDGSYGLALVGCCAVLLAPELAGKAG
                     RTLLRYDRAEIAAGEWWRLLTAHFVHLDLAHAALNSLGLVLMWALFARDYRPRQWLTI
                     VLGAIAAIDAGLWLRDSTVAWYVGSSGALHGVMAAGTLAHLRRRDLDGWLLVAFIVIK
                     LVYEQVAGALPFTDSAEAVVVDAHLYGAVGGLVVGACLRSPLESA"
     gene            27603..28142
                     /gene="gspG"
                     /locus_tag="VFB37_03735"
     CDS             27603..28142
                     /gene="gspG"
                     /locus_tag="VFB37_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HZO21591.1"
                     /translation="MDRKQDAFEVTRAGQVPGGPGGLGTAQHRRAPWRRGSPRASGFT
                     LIEIMVVVIIIGLLAAVIVPQVVNKVDEARVAKAKQDIQSLETALTMFRLDNSKYPTT
                     DQGLQSLVKQPTDPSIRHWRQGGYLERISKDPWGNDYQYVYPGTHGKEYDLYTLGADA
                     QPGGDGVNADIGNWNIGDQ"
     gene            28157..28825
                     /gene="gspH"
                     /locus_tag="VFB37_03740"
     CDS             28157..28825
                     /gene="gspH"
                     /locus_tag="VFB37_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HZO21592.1"
                     /translation="MRAPGRSAGFTLIEILVVVLIIGIISLGVLLSVSLTGRDRELET
                     ESDRLLSLVNYAREQAELQTREYGVIFHDDGYQFVAYDQRRGHWREVYEDEALRQRKL
                     PLGLDFTLSVESRPVVLVPTGEAKHDKPKSKAKSLKDISTLDDAVSKDSKDSSSGLRG
                     QEDSNATLEDEKPITPQIMIFSNGDLTSFEVTLERDGGTRSVTLAQDDKGQVIEKPMI
                     EHKT"
     gene            28831..29391
                     /gene="gspI"
                     /locus_tag="VFB37_03745"
     CDS             28831..29391
                     /gene="gspI"
                     /locus_tag="VFB37_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HZO21593.1"
                     /translation="MTRGFTLIEVLVALVIVAVGMAALMGALSSSADTVNYMRDKTFA
                     EWVALNQLANLRLQMQPGQVPATGNTNGDLDYAGRSWHWRQEVVATQVQGMERIDVKV
                     RPAEVKAGDDAGWFVTVSGIVGDAVAAPGSATLSWGTGVTQPGNTNGNGTLQPGSTTT
                     PGTTTPTTQGPQPSPTPTKPLGGTSP"
     gene            29494..30072
                     /gene="gspJ"
                     /locus_tag="VFB37_03750"
     CDS             29494..30072
                     /gene="gspJ"
                     /locus_tag="VFB37_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HZO21594.1"
                     /translation="MFIAAVIFAMGYGAVNQALKSRVELQEQQAKLLQLQTTMRVLEQ
                     DFVQLAPRPVRQPVGDSYQPALQSNPSAPPLLALTRGGWTNPTGLQRPGLQRVAYYFE
                     NGTLRREFWTVLDPTQNSTTIKRDLMTHLKSVTLRFMDLSRVWQPQWPPATNTVGATN
                     ADLMLRSRPIAVEITLETEDWGKVVRVVEIAG"
     gene            30069..31091
                     /gene="gspK"
                     /locus_tag="VFB37_03755"
     CDS             30069..31091
                     /gene="gspK"
                     /locus_tag="VFB37_03755"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HZO21595.1"
                     /translation="MSSRALVPTRRRYDSAAPGRQRGIAMLVAILLVALGTILAAAIA
                     YESAMSARRSTATFSFDEALLVDEGAEALAAYGLRAIRQQDKTGTINMAQPWVQPVGP
                     VEVVPGVTLEASLEDLQGRFNLNSLVLSDGTGQPDPQAVQIFQRLLQLVGVEPKWADL
                     LVDWIDPNNQPLPDGAEDNAYLSQDPPYLTANLYITSTTELLALPGFGADRFRKIAPY
                     VVALPPTVTQVNICTASGVVLDALMAAGGQGEESRAEFSLDPQGLAKNRASAGMCFPS
                     LQEYQAALGSNASKATKYVGTNSHYFRLSSFVTLGTTEFNLYSLLFQDDNLGMVHTVL
                     RSYSPD"
     gene            31112..32353
                     /gene="gspL"
                     /locus_tag="VFB37_03760"
     CDS             31112..32353
                     /gene="gspL"
                     /locus_tag="VFB37_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HZO21596.1"
                     /translation="MADWLLLRMPRTPEELATWAVVDPRGNQVGPPQSGPLALAAPRA
                     AGRRICVLVPGSDVLLAEPDVPMKAGTKLQQIVPYALEEQLADDIDDLHFAFGKRIGE
                     SSRTPVAVVRRDLMNEWLQTLKESGIAPDTMYADSDLLPHNPGQAVALLEEGVVIVRP
                     PSGSPVTLPTEALGEALNIAQQATAELTAGGGRGLVLYTGAAEWHQHSAQVEALRERF
                     DGIKIQLLTGGPLTLFAQQLPVASPVNLLQGTYAPVTQNRVGWQAWRIAAILLVCLVG
                     LHIVGKTAELGLLKRHERALDSSIGETFRAAMPGEHSMLDARRRMEARLAAARSGGDS
                     GGLLSALEALGQARSVAPGAVVQALSFHQGALELKLSAPDATSLDKMSQALRGNGWDA
                     SLTSGNAVGSAYEGRIQIRPR"
     gene            32366..32836
                     /locus_tag="VFB37_03765"
     CDS             32366..32836
                     /locus_tag="VFB37_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HZO21597.1"
                     /translation="MPTLSLHNMSEREQRMVLIGAILAVLLLIFAVLVPLDHSVSKAR
                     ERITRKQADLAWMRSVAPELAAAGPGQLPASNESLLVIVDRSARESGLGAALAGSDPS
                     GAGGLQVRMQKASFDAMVGWLARLSQQNGIRVDGATIDNAGAPGIVNAAIILQR"
     gene            32863..33600
                     /locus_tag="VFB37_03770"
     CDS             32863..33600
                     /locus_tag="VFB37_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HZO21598.1"
                     /translation="MNRSIWLAVAVVLVFAVILIARLPAAWVLSKPGSDLSCADTAGT
                     IWNGTCTGMVVQHQPIGDLNWEVHAWRLLSAKFAATVLLTRAAGVARGTVEVGFGGHL
                     TGHDIHVDLPLDPRVMPQLPPNLSGSVQANLSSIQMKGNTVTAVEGEVQALNLQLGTG
                     PGAEPLGSYSLTFSPSSGAPIGQLRDLGGPLQVEGTLRLTPEPGFDLQSLVKPRANAP
                     EALTRDIQFLGSPDAQGRRPFALAASF"
     gene            complement(33627..37010)
                     /locus_tag="VFB37_03775"
     CDS             complement(33627..37010)
                     /locus_tag="VFB37_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZO21599.1"
                     /translation="MAEQGDLFAIEEPGEGLCDERAREYAADPHRSVLLQAPAGSGKT
                     TVLTERLLRLLAQVNEPEEILAITFSRRAAAEMRDRVLKALRGAMEDGSAAPALHAPD
                     ALSQRLRSLAAAALARDSARGWNLAQDPGRLRIQTIDSFNFTLASQLTVTARAGGSLS
                     ITERPEELYAEAARATLASAEDDPQLALDVELLFERLDNNWRNVEQLLADMLQRRGHW
                     LRYVLGHEPHALCARINESLGHIVREQLVATLRRIPGDLLAQAAALAGVGRLGAEPET
                     LGEWKRLGCLALTGQGEWRKSSGLTRAQGPGYELAATREALAAVIERMSDVPGLRFTL
                     LDVAALPSSTLATADAGAIEALSRVLRFAAAHLQAIFAAAGRVDYTYVAGAARAALIE
                     AGLPTELALRTGLALRHILVDEFQDTSLAQLELIEGLVAGWEEGDGRTLFVVGDPMQS
                     IYQFREAEVGLFLRARNEGIGSVPLQALRLTRNFRSIPELISWTNAAFRSLYPETDDL
                     RASAVAFTASEPGTQGAAACPGEPVRLRFFADRDAETRAIGERIAELRAGDPQATLAI
                     LVASRSHATVLMAELASRHIEAVGIELVPLSELPIVRDLVALTRATSHLGDRSAWLAL
                     LRAPWCGLSLRALTALSGRRHPLLVWEALEDPACLSDCSTEDCLRLARVRDVLSSALS
                     TRHTLALVDWLELTWLRLGAADAYPASELAHARAFFDALSQRVASVEWTGAQDLDSLL
                     RDLYAQPQASSANPVQVMTIHRAKGLEFDHVFVPALDRDLNRGREPLLRWLDLPRAQG
                     DSDLIMAPVPPVGPEGPGEVTTFLKRLAAQRAANERTRLLYVAATRAKKSLHLSAAPR
                     PKANGTLTARAGTLLATLWPALDPLALRRATSEEFAGQSAAGAGPETTPSESAERQAS
                     THLRRLVSRWSPPALARGPELSRLPLSRQSLEPPEFSWVQETARHIGTVVHAALESFS
                     RAAQLPTAAAIEAQRGDYLQQLRRHGVPQRDLPRAAERVIDALKATVSDERGRWIFAP
                     THREARSELALTGLAAGRLVNVVVDRSFLDADGNRWVIDFKTSRHEGGGLEAFLEQEM
                     QRYRPQLETYRTLARHLGEHPVRAALYFPLLATFRELEG"
     gene            complement(37003..39723)
                     /locus_tag="VFB37_03780"
     CDS             complement(37003..39723)
                     /locus_tag="VFB37_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZO21600.1"
                     /translation="MIRLEESISAAVEAGGTLVVPSRQRAHAARLAYAAAQLARGRHV
                     WASPQILAVEGWQVREIERAAAAGQSLPRLLSPAEEWLLWRQCTAVATESLELLDRPG
                     LADGLRQASALAADFGLDVASFAKAPGLESPLLCEVQGAVDARCLSLGAARMHSLTAS
                     LSGACRARTTVFAGFTKLPPRLVSLGAVQPVSARSLATPRVVRAADETEELERIALWC
                     QDLLSGPAQARLLVILPGAPGSRERLATLIRQTVDARAWLDSGAARPADEPIGNLAVI
                     EGGSGLARIPVVAHALDLLSWLAGTPGQLNALSEWLRSPYWSTPEEGARARIDLWLRE
                     RSLTRVDLRNWRAIVVSAPAELVRPVQEIASKLDLAAHALREGQASPRDWSERFRAAL
                     EAAGWPGSRVRGSGEQQTVVRFHELLDELGQLALASRSMSLQDAVHWLTELAARANYR
                     PADEDALVTIAPALLDPVVSYEGIWVAGLHADVLPRPLQPDAFIPLALQTLSGIPQAS
                     SAGRLQEAHALMNAWRAATNHLVLSVPRRSGDLELLPSPMLSAWGQGTQPMGTDRVDR
                     AGDMAATDRAAVWLAARLHRPNRLECIDDPGLAWPAGQLLPSGTRSLELQNLCAFRAY
                     AELRLGSKPMETPEPGVALDVRGQLLHAALEKLWQSLRDSRALAAHSESSLGELIERC
                     VAEAAQRVLAPRYGGAASRGRERLLAREHRRTVRLIKGLCALERERKPFRVQATEAEA
                     TLTLAGAPMRLRIDRLDELEDGGHAILDYKSGRRQPGDWYSERPSHPQLLAYLAAHRN
                     TAVATATVNVTAREIRFDGIADSAGRLPRVKGVQATGMAARDAWSLRTLEWLTKLENL
                     TRDFLAGRAVLDPKPGACDFCHAIGICRISERALERLSELNELSLPPSDG"
     gene            complement(39748..41061)
                     /locus_tag="VFB37_03785"
     CDS             complement(39748..41061)
                     /locus_tag="VFB37_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO21601.1"
                     /translation="MKTEIGFRPSEGLQHVRYEIRGRLARRAHELERLGYEIISLNIG
                     NPGAFGFRTPETMRLAMIENLPEAEGYCHQKGIFPAREAVIMQQQARGVAGVTAEEVF
                     IGNGVSELIDLVLRALLNDGDEVLIPSPDYPLWTAAVTLNRGRAVHYPCRAENRFVPD
                     PEEVASLVTARTRAIVVINPNNPTGAVYPRAVLEALVQLAERNGLIVFSDEIYDQMVY
                     EDAEFVPMATLVNDTLCATLSGLSKIYRACGYRVGWAVFSGKLAAATDYLNAMELLSS
                     LRLCSNVPAQWAVQTALGGYQSIRDLVGAGGRLHESRRAILSAVQQSQFLHLRPPAGA
                     MYGFIGVDARELPGFDDQQFALDLLEQKHVLVAPGVSFNVPYRTHFRVTNLPDSATLQ
                     TVFARIEELLLGYASGARTRAGSPGSAQLTPVTAERVVQVSERFR"
     gene            complement(41097..>42885)
                     /locus_tag="VFB37_03790"
     CDS             complement(41097..>42885)
                     /locus_tag="VFB37_03790"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZO21602.1"
                     /translation="EVSFQTPQSEVHAVRSLSLSVAPGECLSVVGESGAGKSQAFLAI
                     LGLLAENGRARGHAWFAGRDLIASSRVELDRIRGARIGMIFQDPMTSLTPHLRVGEQI
                     AEPLVRHRQLSWRCARGRALELLEQAQVTDAARRLHQFPHELSGGMQQRVMIAIALAC
                     EPSLLIADEPTTALDVTIQAQILSLLAELKRKRGLSMVLITHDLGAVAGLADRVAVMQ
                     AGSLLEIDSAHTILTRPQHPYTQELLKAVPRLDAAGPAGTDSEGPVTLQAADVRVQFE
                     VRTSRLGRAATLHALLGVDVQLHAGEALGIVGESGSGKSTLARAVLRLLRPQSGSIAW
                     LGRSIESLPGAVLRPLRSEVQIIFQDPLASLDPRRTAGESIAEALQVHRPEMGAAQRL
                     QTVHEAMLRVGLAAELLNRYPHELSGGQCQRVGIARALVLRPRVLVCDEAVSALDVSV
                     QAQILRLLAELKRDEGLSILFISHNLAVVKQLCDCVLVLYLGRMMELGRAGSLFGRAL
                     HPYTRELMAAAPTPDPAVQPGRLLRILAGEPPSQLALPSGCVYRTRCAHAIEICRERL
                     PAWEEGGAPRQRVACHRWRELAALSDAGD"
BASE COUNT         7600 a        14299 c        13720 g         7266 t
ORIGIN      
        1 gccggggccc atccgggtaa tggtggatgc cacgccgtgc ggaacgctgt aaattaccgg
       61 ctctcgtgca ggtaatggca ggcctaggtt cgtcatgcgc agacgtccgc acgccacgct
      121 cggcggcatt ctggtgggtg gctggatcgc cggaacgctc gatattggag ctgcggccct
      181 catcaatcac atcagcccgc tcatcatcct gcgattcatc gccgctggcg tactcggact
      241 tcagtcgttg aaaatgggcc tgacttcttc cgctctcggc ctgctccttc aatgggcgat
      301 gtcgattctg atcgctgcga tctgcatcct cgtgacccag ccgattgccg gcatgcggcg
      361 tcactggatt ccgctcgggc tggcttacgg cgtcgccaca ttcttcatca tgaactacgt
      421 ggtggtcccc ttatcggcga tcgggcacgg accgaaattc acccccgagc acttcgccgc
      481 gaatctgctc gctatgttgg tgtttggcct gatcatcgcg ttcttcacgc gtgcggccgg
      541 tggtcatttc gcgctcggtc ccctccccgg ccgaccgccg gctcgatcct gaagcgcaca
      601 ggtacctcgt ggagaggggt cgctgagctg aggcgatgag gtcgcgcggg ggcggctttc
      661 cgcgccgttg ccgcttggga acgcgagatg ctgctactcg tggatggccg gcatctcgct
      721 gcgatgaagc gactttcacc agatgaggac gctgctcacc gggtgctcga gatcacgcgt
      781 gcctggatca aggaagcggt gactgagctg cggaaaagca cgctcgatga cgaagtagtc
      841 cattcggctc gcaaggcact gaagaaggcg cgcgcgggac tgcgaatgct acgcccaggc
      901 ctcagagatg ccgagtatcg caacgccaac gcggccttgc gcgatgcctc ccgccctctc
      961 ggtgagttac gtgacgccaa agttctgcag gacaccctga cagacctcat gagacgccgc
     1021 cgttcacgcc gccggaacgg gctcaatgtg gcccgcgtaa gcagcctcct gaaggagcac
     1081 cgcagacggg ttcggcggca gatcctgcat gacaagcgag ccctcgctca ctcgcgccac
     1141 ctcctgcgag tgacgctgtc gcggacgaag aaatggagac tccgcaaaac gaactggaag
     1201 ctgctcgggc ccgccctgga gcatgcatat cgcaaggccc ggaaggcacg cgctgatgcg
     1261 tggactatcg gaaccccgga agcgctgcac gagtggcgca agcaggtcaa ataccttcgt
     1321 tatgcgctcg agatcgcgga accgctctgg ccggccatgc tcggggtgct cgccaagcag
     1381 gctcatgagc tctcggacga gctcggcgac agccacgatc tgacggcgct ttgcgaaatg
     1441 acggtcgccc atccagatgc attcgcaaac tcgagcgagc tcgatgcgct gcttgcattg
     1501 agcgagcacc gtcagcagga gctgacgggc gaggctaacc tgctgggtgc ccgattgctc
     1561 ggcgaaaggc cgccggcctt cggcgctcgc ctcgagggct actggcgcaa ttggcaccaa
     1621 ggcgcaggtc tcgttcacgc tcactgaccg cgcagaatgg actcgactgg tggcacgcgg
     1681 cttgcgagaa ctgtgcagcg tggcgctcct cgtcggggga ccgccgttaa ttgaaaggag
     1741 cggtctatga gtgcggtttt gacagcgttg tactcgaatc acgagaccgc ggaacaggta
     1801 cggacacggt tggtccagga tggatttccc accgaccgcg ttcagctcac ctcccgccag
     1861 gagctcgggc aagctgacgt cgcccccgcc aaggagctgc ccgaaaagct caaacagtac
     1921 ttccaccagc ttttccagaa tgagcgggcg cagcgctcgg cgcaacgcct ggagcgcgcg
     1981 gtactggacg gtggcgcggt aatcgccgtg catccgcgcg gggatgtcga gacccagcgg
     2041 gcgctcgctt tgatggagga ctcggggccc gtggaatttc gtgccagcga cctgcagaac
     2101 caggccggcg agcacgccgc ctcgagcggc tccgaggcgc tctcctggat cgggaagatc
     2161 atggtggcac cgctcgcgcc ccaccggacc agccccggcg cggggggctg acgcggctcg
     2221 ccagaggttg cgggcgtccc tgccacgttc gtctctcagc tggcctccac cgactccgga
     2281 tgcaactccg cgctgctctc ctttgaggga gcctccgcgg gctggccttc acgtgcgccg
     2341 ctggcccaca cggtacctgg agcgatcagc atgttcgcac gcaagccagg atggttatcc
     2401 gcaagctcca gcgcccctcc atggaccttg gccaccgagg cgacgaggct caacccgagg
     2461 ccgactcccg gcgtgcctcg gctcgtgtcc ccacgataga agcgtgttgg gaccttgggt
     2521 ttttccgaat cgggtattcc cgggccgccg tcggcgaccg agatgcgcac tcgtccgtca
     2581 gcgagcgtct ggacctcgac ctctatctgt cccccctcgc ccgcgtactt cagcgcgttg
     2641 tcgatgagat tgccgatggc ctgagcgagc agcacgggat cgccggagat gccggccggt
     2701 cccccgcgat acgacaggct gatacccttc agctccgccg caggcagata gaagtccact
     2761 gcctgccggg cgatgtctgc tgcattgatc gcgacgaagc ccgagcggcg cattccagcg
     2821 tcgatctcgg cgaggcgcag cagcgcattg aagatccgga tgacgcggtc aacatcgacg
     2881 accgccgcat cgatctccgc gaaggtctcc tccgcctcgg ggcgggtcag cgacagttcc
     2941 tccaggcgcg agcgcagctc ggcgagtggg gtacgcaggt cgtgagcgat ggaattcgaa
     3001 acatcccgga caccgtgcag cagctgctcg atctgatcga gcatgccgtt gatcgtgcgc
     3061 gacagggtgt ccagctcatc actgctcggc ttggtcggca ggcggtggct ccagtccccc
     3121 tgcacgatcg cgagcacagt gtggcggatg ttgtccacgc gcagcaggag tgctcgccgg
     3181 accatcagtc ctccgagcac accgacaatg gacagcactg cgaccgccgc cgcgagccca
     3241 taccagaagc gggtcgtcag aggggcgaac aaagtctgat cgcgaccgac gagcaggttg
     3301 taaccgcccg gcaaaacggt atgcacgaac atcgccttcg tgtgatggcc cgccagattc
     3361 accggcaggg tataggttcc gggctcaacc gggatcacac gcggccactc gggaagattg
     3421 ccggccacag gatgcagcga gggatcggtg aggagcagga tccgctcgcc ggggatctgc
     3481 atgcccacgc gcgtgttgat gaaagctttg agtccggatg ggccttcgcg cgcgaacact
     3541 tccgccaggc gctgcgtatc gacccgcagc agctccgtgc ggctgtcctg gatggtgacc
     3601 tgccacgcat accacagcgg agctgcgaac aggatcagcg cgccgatccc gaaagcgacg
     3661 tagctgaagg caagagtgaa ggctgatgag cgcacgatcc tattcatcgg cagcgagcat
     3721 gtaccccgcg ttccgcacgg tgcgcaacag cggccgctcg gagtcggatt cgatcttctg
     3781 gcgcaacttg ctgatgtgga cgtcgatcac gttggtctgc ggatcgaaat gatagtccca
     3841 gacgttctcc agcagcatcg tgcgcgtcac cacctgccct gcatgccgca tcaggtattc
     3901 cagcagcttg aactcctgcg gctgcaacgc gatcgcacgg ctgccgcgag ctgctttgcg
     3961 cgacagcagg tccatgtgca gatctcctac tttgaggctc gtctgggtcc caccgccctg
     4021 cacccgacgg cacagggcat ccagcctggc caggagctct ccgaaggcaa acggcttggt
     4081 gaggtagtca tctccgccgc tcttgagccc gcggatgcgc tcatccacat ccgatagcgc
     4141 gctgaggatg agaatcggcg tgcgattccc gagcttgcgc agcgcctcga tgatggcgag
     4201 gccgtcgatg tctccgggca gcatgcgatc catgacgatg acatcgtagc gctcgctggc
     4261 ggcgaggaac atgccatcgc ggccgttgtc cgcgtgatcg gccgtatggc ctgcctcctg
     4321 cagccccttg accacgaagc gcctcacccg ctcgttgtct tcgaccagga gaaccttcac
     4381 cgcatcgctc cgatagcctg ctgcatggct cagaccgtcg ccggctcgtg atgcgtgtcg
     4441 tcggggcgat ctgcatcagc gcccaggcga tgtcggctga gccgagcatt cacacgatcc
     4501 agatacaggt agaccaccgg cgttgtgaac aaggttagag cttggctgag caaaaggccg
     4561 cccaccatgg tgtaccccag cggctggcgc agctccgagc ctgttccggt tccaagcatc
     4621 aaaggcagcg aggcaagcag cgccgccatg gtcgtcatca tgatcgggcg gaagcgcagc
     4681 agacaggcct gccgaatggc ggcttccgga ctcaggtgct gctcgcgctc ggcctggatg
     4741 gcgaagtcca ccatcatgat gccgttcttc ttcacgatgc cgatcagcag gatgatgccg
     4801 atcaacgcga tgaccgacag gtcgaagtga aaggccagca gcatgagcag cgccccgacc
     4861 cctgccgagg gcaatgtcga gagaatcgtc agcggatgaa tgtagctctc gtagagcatt
     4921 ccgagaatga tgtagacgac gaccagcgcg gcgaggatca gatacggctc gctgccgagg
     4981 gaggactcga aggcctgcgc ggttccctgg aacgtgccga gcatcgatgc gggcatgccc
     5041 atcgtggtgg caattctgtg aatgccatcc actgcatcgc cgagtgcggc ccctggcgcc
     5101 agattgaatg acagcgtcac cgcagggaat tgtccctggt gattgaccga caagtagctc
     5161 acgtgctgcg tgtcatagtg cacgaacgtg gacagcggca cctgttgatt cgtgatgggg
     5221 gatttgatgt agatcttgtc gagcgaatgc ggatccttct gcagttgcgg tgtgatctcc
     5281 aggatcacat ggtaagcatt gagctgcgtg aagtactgcg ccgcctgccg ttgcccgaac
     5341 gcatcgtaga gggtctggtc gatgagcgca ggctggatgc caaagcgcgc cgcctgatcg
     5401 cgatcgatga cgagcgacag cgtggttccg ttggtctgct ggtccgaggc cacatcgcgc
     5461 aacgcgggta gcttctgcat ctgcgccagc atcttcggcg cccactcgtt cagctcatcg
     5521 agattcgagt cctgcagcgt gtactgatac tgagtccggg tcgtgcgtcc accgaccccc
     5581 agatcctggg acgcttgcag gaagagcgta gcgcccttta cgttcgccag cttcgcgcgc
     5641 aggcgcgtaa tgacctgatc ggcagtcgcg gtgcgctcgc tgcgcggctt caatccgatc
     5701 cctacccatc cgctgttgac cggatgcgag ccgccgatgt gcgtcgccca gctcgcaacc
     5761 gccggatctt cgttgacgac ctccatcagc tgcttctgca gcttcatcat ctcgtcatag
     5821 gagacatcct gcgaggcatc cgagagcccc atgaggatgc ccgtgtcctg ctgcgggaag
     5881 aagcccttcg gtacgaaaac gtacagcagc ccgctgagca cgacggtcag cagaaaaacc
     5941 accagagtcg ggcgctggtg agtcaacacg aaatcgagcc cgcgtgtata gccccgcagc
     6001 actttgtcga atcccttctc gatcgtcaga tacagccggc cgtggctcac ccgatgctca
     6061 tcgcgcagaa acagcgagca catcatgggc gagagcgtca gagcgacgaa tgccgagacc
     6121 gcgatcgtca tggtcacggt gatcgcgaac tcgcggaaca gacggccgat gatcccgccc
     6181 atgagcagca gcggaatgaa caccgccacc agcgagatgc tgatggacat gatggtgaat
     6241 ccgatctcgc gcgagccctt cagggaggcc tgcaacgggg aaaggccgga ctcgaggtgg
     6301 cggtagatat tctccagcat gacgatcgca tcatcgacga cgaacccgac cgcaatcgtc
     6361 aaggccatca gcgagagatt gtccagcgta taaccgagga cgtacatgag agctgcggtt
     6421 ccgaggagcg ccagcggcac cgtcaccccc ggaatgatcg tggcccagac gttgcgcagg
     6481 aacagaaaaa tgaccagcac aaccaggacg atcgtcagta acagcgtgta ttccacgtca
     6541 tggaccgagg cggtgatggt ggtcgtgcga tcgatgatcg tgctcacctt gatggacggc
     6601 ggcacggagg ccatggcatg cggcagcagc gcattgaccc gcttcaccgt atcgatgaca
     6661 ttggcgccgg cctgcttgaa caccatcaac agcacgccgt caccgccctt ctggaatgca
     6721 aacgagagtt cgttctcggg cccatccacc gcgacgccga tatcccggat acgcactggg
     6781 gcaccgttct tgtacgcgac gatcacgtca ttccagggag cggccttcag gagctggtcg
     6841 ttatcgtaga tggcaaagct gtggtgcacg ccgttgatcg ctcctttcgg agcgtcgacc
     6901 gtcgcctcgg agatcacgct ggcgatgtcc tcgagctgaa tgccaagcgc tgcgatcttc
     6961 gccggatcca cctgcacgcg gatcgcaggc ttgcgctggc cgccgagcga gacctgggcg
     7021 acgccgggaa tgcgggagat ctgctgggtg aggacgttct cggcgtagtc atccaccacg
     7081 gtcagcggca gcacgtccga gtgcaccgag aaaatcagga tcggtgagtc cgccggattg
     7141 accttacgat aggaaggcgg cgagggcagg ttcttcggca gctggccgcc ggcggcatcg
     7201 atcgccgact gaacatcctg cgcggcggca tcgatgttgc ggccgagatc gaactgcagc
     7261 gtgatctgcg tattgccgag cacgctggag gaggtcagct gcgaaacacc ggggatctcg
     7321 gcgaactggt attcaagagg cgtcgcgacc gcggaggcca tggtctcggg actggctccc
     7381 gggagattgg cggacacctg gatggtgggg aagtcgacct gcggcagggg tgccacgggc
     7441 agcagcgggt aggcgacgat gccgatcagc agaattcctg ccatcagcag cgaggtggcg
     7501 atcggccggc gaatgaaggg ttcggagatg ctcactggac agttcggccc agggtggatc
     7561 gcttgcgggg cgggggcgtc gcggccatcg gcggggatcg atcgggaacc gtggccacct
     7621 caggaccgct atcgcctccg aggctcgagt tactgctgat ctgcacccgc gcaccgggct
     7681 gcaggcggaa ctggttgctg gtcaccacac gctcgccttc gtgtagacca tcgcggacca
     7741 ccatcacggc gccactctcc tcggcgacct tgagcgggcg ggcctccacc gtggaatcgg
     7801 gtttgatcac gtaggtgtac atgccgttcg gaccgcgctc cacggcgttg gagggaatcg
     7861 tgagcgccgc gtgctcggtc cgcacgagca cccgtgcggt gaggtactga ccgggccaaa
     7921 gcctgttctg tatgttaggg aatacggcct tgaggcgaat cgtgccggtt gactggtcga
     7981 tctgattgtc gatcagtgtg acggtgcccc gatcgagctc cgtgccgcca tcgcgcgaga
     8041 gcgccacgac ctgcactttc ccagcgctca gagcgctacc cacggagtcc agcgtttcct
     8101 ccggcaaggt aaagatcagc gcgatgggct gcatctgagt caccaccacg atgccgttcg
     8161 tatcggtcgc gtgcacgtta ttgccggcat caacgcggcg gatgccggtg cgcccctgga
     8221 tgggtgaggt gatggtggtg tactgaagtt gggtctgtgc attctcgatg ctggcctgat
     8281 cacccttgat ctgcgcttcg aggccggcca ccagtgcccg ttgcgaatcc agcgtctgct
     8341 tgctcgcgag gttctcgggg gccagcacgg tatagcgctc caggtcggcc cgcgcgcttt
     8401 gcagttgcgc ctggtctttg gccttggcgg cttgcgcctg ggccaggaca gcctggaacg
     8461 gccgcggatc gatctgcgcc agcagatcgc ccaccttgac catctggccc tcgacgaagc
     8521 cgattttctg caactcccca tcgacccggg cagtcaccgt cacggtgttg aaggcttgca
     8581 cggtaccgag cccgtcgaga tagaccggca cgtcctcgcg tttcaccgcg gtggcgtcca
     8641 ccgcgaccgt ttggttgggg tcgcgcggcg gcgaggccgc ttcggtgcgc gtgccgggca
     8701 ggccgtgcca gcccacgatc agcaatacca gagcgcccgc acccagcaac cacacccgcc
     8761 ggcgatgcct tgtaagcatt ccgcgaaatc cgttcggtag tgtattcaac gtcaccaact
     8821 ccacatctca gtgttacgcc tgggcagccc tcaaccgtgg ccggtttgcg ggctttgccc
     8881 ctgttgccac cctcccccta gcgcgctgaa gagctggacc agcgtctgaa agtgcgcgaa
     8941 ccgcgcctgg acgagctcat cttcagcggt gaacagggct gactgcgtat tcaaaaccgt
     9001 caggacattc acagtcccgg catgcaactg gatctcggtg atctcgtaag cgcgacgcgc
     9061 cttggcgact gcttcctgct gacgtcggta ttgctcggcg gtctgctgcg cggccaccag
     9121 tgcgtcctcg acgttgctga aggcggagat caccgctttg tgataatcgg ccagcaattc
     9181 ggcgtagcga cccttgttga gccggtaatt acccaccagt gcaccaccgg cgaaaatctg
     9241 ctgagtgacc ccggcgctca aggaaaatat ccgattgagg ggcgtcacca ggctcgagag
     9301 cgccgtgctg acgaagcctc catctgccgt cagatcgatg ctggggaaga aagcggcccg
     9361 cgcggccgcg atgttggcgt tggccgacat gagttgcgcc tcggcctcgg ccacatcggg
     9421 ccggcgtgcc agcagctcgc taggcaatcc tgccccgacg accggctcgg acagatcggt
     9481 cagcgtgctc tcgggcgcat cgagctcctg gggcatgcgc ccgacgagca ccgccagcgc
     9541 atcgatcgtc tggcgcagct gttgtcgcag cggcggaatg gccgcgttca gcaccgccac
     9601 ggtggtctcc tgctgcgcga catccagcgc agccgcggtg cccacctgct cctcgagctg
     9661 cagatccttg agaacggtct gggccgcaga cagatcctgc tgtgcgacgt tcaagcggtc
     9721 atgcagctcg atcgcctgaa aataggtgtt ggccacactc gtcatcacac tcagctcgat
     9781 cgtggcgcga tcgtagcggc tcgcttgggc tgcgaacttt gcggcattga aggtggcccg
     9841 gttcttgccc cagaaatcca gctcgtagct ggccgatgcg agcgcactga attccgagaa
     9901 cgtgatcggc ggggccccgg gcaaagcgcc cgatgccttc tcacgctgcc gcgaggcatc
     9961 gcccgagaca tcgagagttg gaaacagagg cgcaccggcg atctgcgcct gcgcatccgc
    10021 ctgacgcacc ctggcgattg ccgcggcgat gtcatcgttg gcgcgctgcg cctgcgcgat
    10081 cagatcattc agttgcgtcg agccgaaccc gcgccaccag tccgccgagg gccatgccgg
    10141 agtagttccg agcttcgcag aagccgaggc cccggtgctg ctgcccgcgg gttgctgggc
    10201 cgccgtcttc tcggcggcgg cctctgtcgg cgtctcggtg atgtcggtgc gccatgccgc
    10261 cggtggcggg atctcgggcc gtttgtagcg tgggccgagg ctgcacgcac tcaacagtac
    10321 ggccacaccc agcgccgctg cgccgcggca ccaggaggat ctgcgccgca gggcggcgaa
    10381 gctcgaggaa tctgcacgta tcacgggaat ggatcggaca agcgggcccg gcaacagaca
    10441 atacgggcaa cggggttgac cgcatattgt cggaagatta atttctgtta acgggagcgg
    10501 ttccgcgggc tctccctggt ccgttccagg cctgcccgat cagggcccgt tcacgagtcc
    10561 tggaatgtcg cgggtcttat agacgtgatc gaccaggccc cagtcccgag cctcctcggc
    10621 gctcatgaag cggtcgcgat ccagaacctt ctccacctct tcgtaggtgc gcccgcaatg
    10681 ggtggcgtac agctgcgtca tccgtcgctt ggtcttgacg atgtcctccg catggcgttc
    10741 gacatcagaa gcttgtccct gaaagccgcc cgagggctgg tgcaaaagga tggtcgcatt
    10801 gggcagcgca atgcgctgag cacgggcacc ggccatcaaa aggaacgacc ccatcgagcc
    10861 ggccatgccg atgcagaccg tccagatcgg acatttcacc cattgcatgg tgtcgtagat
    10921 ggcaaacccg ctggtcacca cgccaccggg cgaattgata tagagcgcaa tatccttcga
    10981 cggattctcg gactcgagaa acagcagctg ggcgcacacc agagccgcca ccccatcgtt
    11041 gatcggaccg ttgaggaaga tgatccgctc gcgcaggagg cgcgaaaata tgtcaaatga
    11101 gcgctctccc cggctcgact gctcgagcac catggggacc agctccatca ggctgcccac
    11161 ctcagccccg ccgaggcaca cgccgtggaa gctctcgtct gcgaggcgat ggagtgtggg
    11221 atcgcagcgg cgaaccctgc atggatcact ctcaaaaggg tcccaccggt aatcgtgggg
    11281 aagagctcga acgtcaccgt ggaatccagc ggcggtccgg ctgcggcttc gctcccatcc
    11341 gctgctatgc gccatcgata ctgaagcagc cggttcggct gcgcctccag gatctcgcat
    11401 tcgacccggc cgtcgggggt gccgggaggt ccctgcagag tgaaacgagc gccccgcagc
    11461 gggcggatat cgttcggtaa cagccacttt gccagcagct ccggctcggt gagagcccgc
    11521 cagacccgct cgggggccgc tcccagctca cactcgctca ccacggcatc ccggctggcc
    11581 tctcgcttgt tacccgtggt gccctgctgc aacggctgcc gagtcagctc tctcatggtt
    11641 cgaaatcctc cagaactgtg cgaaggcgtt gcaggcgttc cggccagaag gctcgatagc
    11701 gatcgatcca cccgatgagc ggggagagtc cggcgggttc tgcctggtag agggtgaacc
    11761 gtcctgcacg tcgctcgcgc acaagtccca cccgacgcaa cgcacccaaa tgctgagaaa
    11821 ccgcgggctg cgaaatggag agccgccggg tcagctcgga aacgctgcgc tcgccgctgg
    11881 cgagcgcttc gaacatggcc cgccgcgtcg ggtcggccaa tgtccggaaa acctgtgcct
    11941 cgagcatgcg cttatataag catagactta tatctgtggc aaattgccgt cgcccccacc
    12001 gcagcagtgc cattgttttt cctcgaatct gagctatatt ttatgcgcag gcatctgagt
    12061 gcgaacgagt atcgttccct ctcgcccaac aactcgaaca acaacatacg gcgaagctcg
    12121 cgactcatga gcaccacggc agatcatttt caggcagctc ttctggccct gacgcgctcg
    12181 gggtccatca aggatcgtct gctcgaggcc tatcgcaatc atctggcgca ggtggaggag
    12241 tcagagcttc ccgccgaagt gcgtgaggag tttcgtctct tcagtcaggc cttgacgcgc
    12301 gagccgccct tgctccgcgg cgaggatgcg tcgcgcgcca ccgttcgaaa aatgtcgctg
    12361 catgaggcgg ccgcgatggc atcgatcgtc gtacgcatgt ttgccgccgt ctcgggcggc
    12421 gcggctctcg gcagctggag taagagcatg cggagcgact cgaatgtcgt tccgctgtat
    12481 caggccgaag ctaatcgagc ttgaattcga tcgtgtccca gcgcgtggtc tcttcgtggc
    12541 gcgcctgacg ccggatcgca tcaaccagcg cccgctcatc ccagtcgatc agctcatcgg
    12601 ccagatcgca gagcgccttc ggaaccgggc gctgtgagcc gaacggcttc atcaggacca
    12661 cgggcttctg actcgcctgc gcatagagca attggaacgt cacgaggtcc tgggacgttt
    12721 catgaagact tgacagtccg atcaccacct cggccggtgc gatctgccgg cgcagctcct
    12781 ccttcagggc ctccttgtcg ccctgcgggc gcagctcggg cttgctcgag ttgcggtaaa
    12841 agaagtttcg cgagctctcg aggtactcga agacgcgcag gtagtcatcg ctgttttccc
    12901 aggcgtgggt cacgaagagc cggatcggat tggcctgcga catgaaagga acgccagaac
    12961 aggctgcatg cccgggacag cagcgagtgt cgcacaagct tcgccggcgc gaaactcggc
    13021 aggccgacgc tccgcagcga cggttttacc ctcccgcata ccaagctgcc ccgatagaat
    13081 gcggcgtttg cgataagggc cagctatagg tgcgtctgct cgtctataac atccgctacg
    13141 ccacgggcac cggtccggcc ttctacctgc cgatccccgg ggcaggctac ctgcggagca
    13201 accgcaaggt gctcgaggga atcaccaatt tcattcgcgc cgagcacccg gatgtggtgg
    13261 gcctgatcga ggtggacacc ggatcgatcc gtaccggcat gctgaatcag gccgagcaca
    13321 tcgccggctc catcggccac tattccacgt tccagtgcaa atacggcacc ggctcgatca
    13381 accacctggt gccgatcgtg cgcaagcagg cgaatgcctt cctgtccgca ccgcacgtgc
    13441 atggcgagcg gttccactat ttcgataccg gcatcaagcg gctcatcatc gagctcgagc
    13501 tcgatgatgt gtgtgtcttc ctcgtgcatc tgtcgctgaa gttccgccac cgccagtacc
    13561 agttgcgctc cctgcacgat ctgatcgtcc agtcgcccaa gccggtcatc gtcgcgggcg
    13621 acttcaacac cttctggggc acccacgaga tctatctctt catgcgcgcc gccgggctgc
    13681 gcagcgcgaa ttccgccggc ctgccgtcct tcccggcacg cgtgccccgc atcgagctcg
    13741 acttcatcct ggtgagcgaa gggatcgagg tgaccgattt tcgtgtcccc gatgtgcgct
    13801 tctcggatca ccggccgctg gtatgcgact ttcatgtccg cacggacgcg ctggtgcgtc
    13861 gcacggccgc ctgaggctgc agaatcatga atgactcccc tgcctggagc agcgagcgcg
    13921 atgccgatgg ggtactgtgg ctcagcatcg acaagcccgg cacctcggcc aacgtactct
    13981 ccagcaacgt gctgcgcgag cttgatgcgc tgttgcagcc gctcgcccag cagcctccgc
    14041 gcgccgtggt ggtgctctcg gcgaagaaga gcggcttcgt tgcaggcgcc gacatcaagg
    14101 agttcaccgg catcagcaat gccgaggagg gctaccagct catccgtgcc ggccagcagg
    14161 tccttgacca cctcgaggcg ctccgctgcc ccaccgtggc ggccattcat ggcttcgcac
    14221 tcggcggcgg cctggagctg gccctggcgt gccgctaccg ggttgcggtc gccgatgagc
    14281 gcctgtcgct cggactgccg gaggtgcagc tcggcattca tccggggttc ggtggcaccg
    14341 tgcgttccgt gcggctcgtc ggggtgaaag cggccatgga gctgatgctc accggaaaac
    14401 ccgtgcgcgc ggacaaggcc ctgcgcatcg gcctggtaga caagctcgtc ccggccgcag
    14461 agctgcgggc ggccgccaaa gccatggcga tgcagccccc accgctgcat cgggcgccgt
    14521 tcgccgagcg cctgttgagc tggggagcga tgcgaccctt cgtgaagaat gcattgctgg
    14581 cgcaggtccg ctccaaggcc cggcgcgagc actatccggc cccgtacgcg atgatcgatc
    14641 tgtgggctcg ctggggcgcg cagggacacg ccgcattcga ggcggaagca cgctcgatcg
    14701 cgcagctctt cctctccgag acttcacgca acctggtccg cgtgttcctg ttgcaggacc
    14761 ggctgaagag cctgggcggc aaggtggcgc aggagcagaa gcacgtacac gtggtgggcg
    14821 ccggcgtgat gggaggtgac attgcggcct ggtcggcgct gcgaggcttc acggtcacgc
    14881 tgcaggatcg cagcctggag ctcatcgagc cggcgctgaa gcgggctcag gagctgttcg
    14941 agaagcgcct gcgcgagccc ggtgcacact ctgcggctcg cgagcggctg cgcgcggatg
    15001 tggcaggtga cggagtcccc tctgccgatg tcgtgatcga ggccatcttc gaaaatctgg
    15061 aggccaagcg cgagctgtac gcgcgcctgg agccacgcat gaagcctgca gcgctgctgg
    15121 caaccaacac ctcgagcctg atgctcgagc cgctggccgc gaacctggcg cgccccggcc
    15181 agctcgtcgg cctgcacttc ttcaaccccg ttgcccagat gccgctcatc gagatcgtcc
    15241 aggcgcagca gaccgacgcc acggtcatgc aggcagccac cgccttcgcg cgccgcctcg
    15301 acaagcttcc cgtgccctgc cggagcgcgc cgggatttgt cgtgaaccgc gtcctcatgc
    15361 cctacctgca cgaggccatg tttgccgctc aggaaggagt gcctctgagc gtgatcgatg
    15421 caacggccgt ctccttcggc atgcccatgg gcccgatcga gctcgcagac gtcgtcggac
    15481 tcgatgttgc aagccacgtg ggcgagatca tcgctgccga gctcgggcgg cccgcaccgg
    15541 atctctcccg gctcaaggag ctgattgcag ccaagaagct cgggcgcaag agcggcgagg
    15601 gattctacac atggcatgaa ggcaaaccgc gcaaacccac accacccgac accaccgcac
    15661 cccccgacct gatcgaccgt ctgattctcg tcctcgtgaa cgagtgcgtg gcctgcctgc
    15721 gcgagcggat cgtcgcggaa gccgacctca tcgatgcagc ggtgatcttc ggaaccggat
    15781 tcgcgccatt tcgcggcgga ccactcgcct atgcgcgcag ccgtggcgtg ggcgcagtcg
    15841 tagcgaggtt ggaagagctg gcagtgcgtc acggtccgcg cttccggcct gactcgggct
    15901 ggccacagct cagcgtggca ctcaaatcct gactcggata cggctgtgca tacactcgcg
    15961 ggccggcctg cagcccgtgt aaaccgtgac ggacgttccg gtgcccccga gggcctgtgc
    16021 taccatcgga cataattgcg acgcaccaat ttccggtaga tggcgtcagg cctggagaaa
    16081 tgcccgaaga acgcgaagcc agcgttcaac tgctcaagac gttcgcgccg ctcgacggtc
    16141 tgaagcgcga caatcttgcc gcgctcgcca agaaggtgag cgtgcgaacg atgcccgccg
    16201 gcaggatgct cttcaaggag ggcgataccg acaagcgcac cgtctggctc gtcgcaggca
    16261 tggtggaaat tcgcgaaggc gaacgcacga tcgccatgat tcgcggtggc acgcccgacg
    16321 cccgcaatcc tctgtacccg aaaattcccc ggacggtgag cgcccgcgcc gtggacgaga
    16381 tcagctatct gtcgatcgac agcgagctgc tggatgtcat gatcacctgg gatcagacag
    16441 gcacgtacga agtcgcggag ctgcaaacgc ggctcgacgg gacgagcagc gatgactgga
    16501 tgacgacgct gctgcagacc aaggcctttc accgcatccc cccggccaac attcaggcga
    16561 tcttcatgcg cctgcaacgc ttcccctgcc gagccggcga catcgtgatc aagcagggcg
    16621 acgaagggga ttacttctac gtcatcgtga acggcaagtg cgtggtcacc cgcgagacgc
    16681 ctctcaaccg cgaaggcatc aagctcgccg agctcggagt cggcgacacc ttcggcgagg
    16741 aggccctgat tgccgaggca aagcgcaacg cgactgtcac gatgctgacc gacggcgtgc
    16801 tgatgcggct caacaagcag gacttccgcg agctcatgaa cgagccgctg ctgcaatggg
    16861 tgccctacga gaaggccacg gagatcatcg cacgtggcgg ccgctggctc gacgtgcgcc
    16921 tgccgagcga gcaccagaac ctggccatcg aaggctcgct gaacatcccg ctttacttca
    16981 tccgcctgaa gatctcggcg ctcgaccgca acatcccgta tgtcgtgtac tgcgatacgg
    17041 ggcggcgcag ctccgccgcc gccttcatcc tcgttgagcg tggcttcgat gcctacgtgc
    17101 tgaccggcgg ccttaccaac agcaacatcg ccctgcggcg ctccgcctag tccctcccgc
    17161 cggctgaccc cctgctgcgc cctgcaaagg gtccgcagct tcccccgagc tgacctgcgc
    17221 gtgccgtcga tggcacgtcg ccatcgtgtg cgccctcttt cgcctcggct gttcgcccgc
    17281 cctcctcggc gggtcagccg ccttttttcc gggttcgccg ccgctcgggg tataccacaa
    17341 gccgcttgtg gtacgctgtc gaaaatggta cagaagtcag tcagtcggta cctcgccgcg
    17401 atcggcaaac gcggcggtga ggcgggacgc ggcgcctcga agctccgcgg gggagtcagt
    17461 tactacaaac gcatctcgcg gcttgctgcc cgggcacggg ccgcgaagcg caaggggtcc
    17521 agatgagcga actgaagatc agcgatgagg gggggcgcag cacggggtgg acctggctga
    17581 tcgtcctggc gatggtgacg ctaccggtgc tcgcgtggtt cttcttcatc cccgttgccg
    17641 ccgtcgccct ggtggcggcg tgcatcatcg gcaccgtcgg cggggcagcc cgtcgcgcgc
    17701 gtgcgcaggt acgtgagcaa aggaagcagc agatgcagtc tgcagaggtc gcgctcaacg
    17761 attattggcg accgatgtcc tgaagagctt tcggctgcgc cgccgcagcg caagagtgtc
    17821 gtcgagcggc ccgtgcggtg tggtgcacgc cgcgcgggag actcacatcg tgtgtgtggc
    17881 tttgtcgccc gagagcgcgc cggccagata gttctcgatt ttcttctgcg tctgcccgcg
    17941 atcctggtcg ctaaactgca cgccgatgcc ggcggtccgc ttgccctgcg cgcccttggg
    18001 cgtgacccag atgacccgtc cggctaccgg cagcttctcg tcctcgccca tcaggttgag
    18061 cagcatgaaa acttcatcgc ccaggcgata gttgctgttg gtgggaatga acagcccccc
    18121 attgcgcacg aacggcatgt acgcaaggta cagcgcgctc ttgtctttta tggtcagggt
    18181 caacagaccc ggcttgttcc caccagtacg caatggccta cctcgcccgg ctcacggcag
    18241 tgttctcgat cttcggtgcc agatgccgca ggagatcctc cagcgccagt ccccggttga
    18301 tcggcgtctc gagcgcggac ctgagatctc tcaccccgtc cagcagcccg aagagcgccc
    18361 gtatattcaa gaccgtcccg gctccttgca agtaggggac agcgcccact tctgtaaagc
    18421 cgccggtcgt tcccgattgt tcctgaatgc gtttcgtgag ccagttctca aagcatcgca
    18481 gcctgagcgg cagctcggtt cgcgcccacc gctcggcagt ggccgcggga tccagcgcac
    18541 ctcggaccgc ctcgtccagg ctccgttgca cctcgcctga gatctgcacg atgaccgcag
    18601 gatccgcggc ggctgcatgc cagggggcct ctccgagcac atcgagcacg gcattccagt
    18661 ccgccttacc gcgcgtagcc tcgagccagc cgatcgcctc ggcccgagcg ggagccggca
    18721 cttgaattcg ctggcagcgg ctgaggaggg ttgcaggcag ccgcgaaggc tgcgtcgcga
    18781 ccaagatcaa caccgtgcgc gccggaggct cttcgagcgt cttcagcagc gcgttggccg
    18841 caaaccgatt gagcagatcg gccggcgaga cgatagcgag cttgtacccg ccctggtggc
    18901 tcgtcaaagc gagctcctgc gcaagctcgc gcacctgctc gatccggatc tggcgcgagt
    18961 cctcgaccgg ctcgaggctg atgaggtccg gatggtggcg cgcacgcaca cgctggcaag
    19021 cgccacacgc gaggcaaggt gctcgctcgg gatgcgcgca cagcacgagc tgggccaccc
    19081 agccggccag ccactcgccg cccgctcccg gggcctcgtg gatcagcaac gcgtgcggca
    19141 tccgatcggc tgcgtacgct gcctgcagag cggccatctg attgcgcagc cagctcatgc
    19201 tcatcgcggc agatcatcct ccggtgagcc gttcatgaga ccggcgagcg ctgcgatcac
    19261 ctgctcgatc acctcattca ctggacgtgc cgcatcgacg acgcggatgc gctctggcgc
    19321 accgcccgca atggcgagat agcctgcgcg gacacgctcg aagaactcga tctgttcggc
    19381 ctcgaagcgg tccgctgcgg ctccggaacg agtgcgggca cgggcaagcc ccacctcgac
    19441 cggaagatcc agaatcaagg tccgctcggg ctgcagatcg gcgtgtactg cgcttgcaag
    19501 ctgctcgatc cacgccaggt tcatcccccg cccataaccc tggtaggcgc gtgtggcatc
    19561 ggtgaagcga tcacagatca cccattcgcc atgccccagg gctggccgga tcagattgtc
    19621 gaggtggatc gcgcgcgcgg caaacatgag cagtgtctcg gtcaccgccg agacgtgctc
    19681 ggtcccgcgc tccagcacaa tggtgcgcac acgctctgcg agtggcgtac cgcccggctc
    19741 ccgggtgaga cgcgcccgca gcttctgccg gaccagccac tctgcgatcg ccaccgccac
    19801 cgtggatttg cccgcgccct cgatgccttc gagcgtgatg aaacggccgc ggatcatttc
    19861 gtccccggag gtgtgcggtc ggggcctgcc ggctcgtgag agcccgtggc ggtctccgca
    19921 tgaggggccg taccggttgc gccgtgtgtc acgcccgcac gctgtagcgc cctgaggtgc
    19981 tcgaggtagc ttttcacggc cttattgtgc tcctcgagcg tcttcgaaaa atgatgacgg
    20041 ccatctccgg taccggtcgc gacgaagtac agctcgcctg tctcctgagg atgcaccgct
    20101 gcaaggacgg agtcgcgccc gggaaggcag atcggggtcg gcggcagccc ggcgcgcgta
    20161 tacgtgttgt acggtgtgtc cttgagcagg tccttcgttc tgatttcgcc ctcataactc
    20221 gccccgagtc cgtaaatgac cgtggggtcg gtctgcagcc gcatcccctt gcgcagccgg
    20281 ctgacgaaaa cgcccgcgat cctggcgcgt tcctccggcg tgccggtctc cttctcgatg
    20341 atcgaggcga gtgtcaaagc ctcataggga ctctgcaagg gcagatccac gctgcgctgc
    20401 tgccaggcgc tccccagaac tctctgcatc gcgttgtaag ccagggaaag gatctccagg
    20461 tccgctgttc gcgccgcaaa gcggtaggtg tccggaaaga atagcccctc cggcgcctct
    20521 cccggatgtc ccagagccgc catcagttcg gcgctgctct tacccagcag cgtgtgcgtc
    20581 acggcagcgt ggtgatccag tgcccggcgg aaatcagcaa aagtcgtccc ctcgatgatg
    20641 gtgatctgct cgagtacgac cttgccttga tcgaagaggt cgaggatctg cgccgggctc
    20701 gctgcagcgg gaatctcgta catccccgcc tggatacgaa gccgatggcc gctgaggcgt
    20761 cccgagagcc gcagatacac ctcgatcgcg cgcggatggc gcaaggcgcc gaggtttccc
    20821 agctcgctca gcaccgtgcg cacgctggca ccctgctcga cctgaatgcg ggcaatgctc
    20881 tgcgaggggc ccggtgagtg gaacagccgc tgcagccaca gatagcccgc gccggctgcg
    20941 cagacggtga ggaggatgag ggcaagcaat acaaccagca cccggccgcg actgctaggc
    21001 atcgcgaggc tcttcgagca gcggcgtgag ttcttcgagc agacgatgcg tgagggctcc
    21061 gggagccagg acctgctcat cgagctcccg gaccggccag ataccaactc gtgcgttggt
    21121 cagaaagact tcatcggctg cgcgcaggtc ggcgaggtca agcgcgcatt cgtgcgcttc
    21181 aatgcccgca ctggcggcct gccgcagcac gatgcgccgc atgacgcctg cgacgccaca
    21241 aacatccagg agcggagtct gaagagtcga accgcgcaca agaaaaacat tcgtcatggt
    21301 gcctgagacg agccggcccg agctgctgaa caggatgcct tcgctcaaac cgcgattgag
    21361 gcattccgcg cgtgcgagga cctgctcgag ccgattgcaa tgcttcagtc ccgccagcaa
    21421 cggattctct ccgagacgca gcgcgagcgt gcccgccttg agcccagcat gaggcggcgc
    21481 ggagtcttcg gctggccatg cgtatcggat tgtgatccgc gtcgccttct cgcggcctgt
    21541 gaatccgtaa ccgcgcgcca ccgccgagcc gcgcgtgacc aggactttca cgagggcccg
    21601 atcggatccg tgtgcgagct cctcgacctc gctgcgcacg gcggcctcat cgtggaaggc
    21661 gatctgcagc cgtttgcagc cgctcatcag gcgctcgagg tgcagggaca ggaagcgtgg
    21721 gcgacccgcc ctgcaggcaa tcgtctcaaa caggccgtca ccaaaatgca ggccccgatc
    21781 gagcgtcgag accccagcag cctctgttcc gtcgaccaac atcctctcgg agcctgcgcc
    21841 aggcagaaca gacccggggt cgtagcgtgc cggctgcttc cggatcatgt gtccgctctg
    21901 ccgagcgcgt ttaggttgaa cgccgccagc atgccgcgcg ctttcgcgcg ggtttcctcc
    21961 agctcgcgct gtggatctga atcggcaacg atgcccccgc ccgcgcgcag ctccaccgca
    22021 cgccccatca ccgtcatcgt gcggatcaga atattcatgt ccatcaggcc gtcgcggccg
    22081 agatacccga gggagccggt atacgctccg cgcccctctg cctccagctc ggcgatgatc
    22141 tgcatgcagc ggaattttgg acagcccgtg atcgtgccgc ccggaaaaac cgctcgcagg
    22201 gcgccgatcg gcgtcacctc cggccgcagc tccccgctta cgttggaaac gatgtggtgc
    22261 acgtgcgcgt aggattcgat gctcatcagc tcatccaccc tcaccgtgcc cgcgcgacag
    22321 acacgaccca agtcgttgcg ctccagatcg atcagcatga tgtgctcggc acgctccttc
    22381 ggatgcgcgg cgagctcgcg catctccgca tgatcatctc cggggcgacg gctgcggggc
    22441 cgagtccctg caatgggtcg cgtatcgatc cgacggccat ggatacgcac cagtctctcg
    22501 ggcgaggagc tcagtatccg cgctccgcgc cactgcacga gcgctgcgaa tggtgccgga
    22561 ttggcgctgc acaggcgctc atagagcctc ccgacatccg gctcacccga aagctgcagt
    22621 ctccagcgcc tggacaggtt ggcctggtag atgtcgccgg ccttgacgta ttccttcgct
    22681 cgaacgacgc gctcgaggta cgcctccggt acttcctcct cgagcgacac aacgcgtatc
    22741 gcctgcgggt cgggcgcggc gagcgcggcg gtgcttcggg catcggactc gatccgctcg
    22801 agcgcctgcg ccgcctccgg ctcggcgatc atgtatacgc gtccgctggc ccgttcgtgc
    22861 atcaaggcag cccgggtccg cacggcaaag gcgcgccacg gcaagctcgc ggccggaagc
    22921 cgcaggtggg gctccacctc cgccgcgagc tcgtaactca gaaagacaga ccagccgcca
    22981 caaaaagcag ggtactcggg tgtcgccggc ctacgctcgg tgagccacca gttctccagc
    23041 gcctgcagga atccgctgcc tgcaggggcg actccgtctg cccccaggcg tccctcccgg
    23101 tcgagccaca gacattcacc cggcgcggcg accagtatgc tgctgtggct gagggcgccg
    23161 tccgcagcac tgtcgaacag gaagggatag cgatcgggaa agcgcgcggc catgcgtcgc
    23221 agcaccgagg gtgcaacgtc cagatcgcgg atgacaggtg gattgcgagc tcccacggag
    23281 ccctccggag tgctcttgcg gaaccgtacg tcagccggtg aagcggcgga agatcaccga
    23341 gccgttcgtg ccgccaaagc cgaaggagtt cgacagggcg acatcgacct tcattcgccg
    23401 cgccgtgttg ggcacgtagt cgagatcgca gtccgggtcc ggcgtgtgat agttgatcgt
    23461 gggcggcacg acgccatcgc gaatcgcgag aatcgagaag atcgcctcga tgacgcccgc
    23521 cgcacccagc agatggcccg tcatggactt ggtggagctc acggccagcc gcttggcatg
    23581 ctcgccgaaa gcgcgcttca tggccgtggt ctcggccttg tccccgagtt gggtcgaggt
    23641 cgcatgcgca ttgatgtact gaacgtcagc ggcattcagt gaggcgtccg ccagagcatt
    23701 gctcatggca agccgcgccc cctctccgtc ctcgggcggc gcggtgatgt gataggcgtc
    23761 gccgctcatg ccgaaaccga tgatctcggc atagatgttc gcgccgcggg ctcgcgcatg
    23821 ctccagctcc tccatgatga ccgcgccacc gccatcgctg agcacaaaac catcgcggtc
    23881 ccgatcccac ggccgactcg cctgcttcgg tgcatcgttg cgcgtcgaaa gcgccttggc
    23941 ctggccgaag ctgccgaggc cgcaaacggt ggtcgccatt tccccgccgc cggcgatgag
    24001 cacatcggca tccccgtact ggatggtgcg catggcaagg ccaatggcat gagtcgaagt
    24061 cgtacaagcg gtgaccaccc ccaggttggg tcctctcagg ccatacttga tcgacagatg
    24121 gcccgcgatc atgttgatga tcgtcgccgg cacgaagaac ggcgagatct tgcggggact
    24181 gtgtcccttg agatagttgt cgtactcgga ctcgatcgta ccgaggccgc cgatgcctgc
    24241 tccgcagact gctccgcagc gggccgagtc gagcttgctg aagtcgatac ccgagtccgt
    24301 gaccgcctgg atgcccgcgg cgacgccgta gtgcatgaag tcgtccatgc gccgcgcatc
    24361 cttgggcgca atgtactggc ttacgtcgaa gtcgcggacc gggccgccga agcggacggg
    24421 aaacgcggac acatcgaacc gcgtgatcgg tccgatgccg ctctccccgc gcaggattgc
    24481 ttcccacgcg gacttgagcg tgctgcccac tggcgaaatg atgccgagcc ctgtaatgac
    24541 gacgcgacgc ttgctcacac gctacctgta agaagatccg acggatggaa ggggcgcggg
    24601 gacgtgccac cccgccgggc cggaagccgg acgggctgcc ccgaagcgct tgggtcaggc
    24661 tttggtgcgg cgatcgttga tgtactcgat cgcctgctgc accgtcgtga tcttctcggc
    24721 atcctcatcg ggaatctcga tctcgaattc ctcttccagg gccatgacga gctcgacggt
    24781 gtcgagcgag tcggctccca gatcgtccac gaacgaggca tcgttcgtga cttgttcctg
    24841 cttgacgccc agctgctcgg caacgatggc tttgacctgc tgctcaactg tgctcattag
    24901 cgctttgtcc ttatgtggtc gtttaacgca tgctcggtac gagcccggcg cggccagggt
    24961 ccgccagcgc gggcgcggta ttgtagggga acacccccgg ggctgccact cgaacgcaga
    25021 tgcgagtgat tgtttttgct gacgaaactc gggcgccgcg ttcctgcccg agccgcgcgc
    25081 cgtgccgcgg cttgtggcgc gcggccaacg gagcacctcg cagctcaggg catgtacatt
    25141 ccgccattca cgtgcagggt ctgcccggtc acatacgctg cacgcgcaga gctcaaaaac
    25201 aggaccaccg ccgcaacatc cgccggtgtc ccgagtcgcc ccatcggaat ctgtgtcagc
    25261 aacgccgcgc gctgctcctc gttgagcgca cgtgtcatgt cggtgtcgat gaatcccggg
    25321 gcaaccgcgt tgatggtgat gccgcgcgta gcgacttccc tcgccagcga cttggtgaat
    25381 ccgaggatgc cggcctttgc cgcggcgtaa ttcgcctgac cggggttacc cgtgactccc
    25441 accaccgagg tgagattgat gatccgtccg cgacgctcct tcatcatgcg tcgcaggcaa
    25501 gccttggaga gccggaacac cgaggtcagg ttggttgcaa tcactcgctc ccagtcctcc
    25561 ggtttcatgc gcagcagcag catgtcgcgc gtgatcgccg cattgttcac caggatggtc
    25621 ggcatctgac cggccgcatc cagctcctgg atcagggcct cgatggaggc cgcatccccg
    25681 acgtccagca cggcgccccg gccgttgtat ccgtgcgacg ccagagtcgc gccaaaggcc
    25741 gcagcccctt ccgcggaagt ggaggtcccg accacctgcg ctcccgcctc ggccagcgcc
    25801 agtgcgatag cgtggccgat gccgcgtgag gccccggtca cgagtgcaac ctcgttctcg
    25861 agcctacccg cctcctgagt cgtcacgctc gttgctcccc gccggggccg cgctcgccac
    25921 ctgcagccgc ggcctgcgtc gcttgcagcg ccgcaccgat cgactccagg tcatccagtg
    25981 cccggaagtc caaccccggt cgcttttcga tccgacgatt cagccccgtg aggactttgc
    26041 ccggcccgca ctcgatgatc tgagcaacct ggctggcgat ggcccggatg gtgcgggtcc
    26101 actgcaccgg tctcaggagc tggcgggcca gatgagcgcg gatctcctcg ggttgctcgt
    26161 gaaaggcgac atcgacgggg ctgaaatagc gcagccgcgg cgggcgcaga tcgacgcggg
    26221 ccagatactc gccgagccgc tcacccgcag cacgcatcag attgcagtgc gagggcacgc
    26281 tgacgggcag ctcgatggcg cgcttcgctc cgcgcgcctt catcagctcg atggcacgct
    26341 gaacggcgcc gcgctcgccg gcaatgacta cctgtccggg cgaattgaag ttgacggcgt
    26401 ccacgacggc cccttgcgcc gcctcccgac aggccgcctc gatctgcgca tcctccagac
    26461 ccaggatagc cgccatggcg cccgtgccgg ccggaatggc ttcctgcatc gcctggcccc
    26521 ggaagcgaac gagcccgatg gccgcaggca gatccagagc ctccgcgcag gtgagtgccg
    26581 tgaactcccc caggctatgg ccggacacca gcgccggctc cccgccgccg cgctcgcgcc
    26641 acagcctcca gaccgccatt ccggcggcaa gcacggcggg ctgggtgcgt tcggttgcat
    26701 ccagcgcttc ccggggaccc tcctcgacga gcttccacag gtcatacccc agcaccccgg
    26761 acgcctcatc gaaagtcctg cgcacgaccg ggacctctgc gtagccggcc agcatgccca
    26821 ccgattgcga gccttgcccg ggaaagacga aggcgaagga catgtgcgct cctgtgtgag
    26881 agtccgcccc gattatagcc agtccaaggt gcgcaccccg cccggcgctc aggccgactc
    26941 cagcggcgat cgcaggcaag ctcccacgac cagcccgccc accgccccat acagatgagc
    27001 atcgaccacc acggcttcgg cactgtcggt gaacgggagc gcgccggcca cctgctcgta
    27061 gaccaacttg atcacgatga aggccaccag aagccagccg tccaggtccc ggcgacgcag
    27121 atgcgcgagc gtgccggccg ccatgacccc gtgcagggcg cccgaggaac cgacgtacca
    27181 ggcgactgtc gaatcgcgga gccacagacc ggcatcgatg gccgcaattg cgcccagcac
    27241 gatcgtcagc cactggcggg gtcgatagtc acgggcaaac agcgcccaca tgagcaccag
    27301 accgaggctg ttgagcgcgg catgcgcgag gtcgagatga acgaagtgcg cggtgagcag
    27361 gcgccaccac tccccggcgg cgatctcggc gcgatcgtaa cgcagcagag tccggccggc
    27421 cttcccggcc agctccggcg cgaggagcac tgcgcagcag ccgaccaggg ccaggccata
    27481 cgagccgtcg caattgagag acgtgagcag ccttctaacc gccttcacag gccccacctt
    27541 tcccatttgt tatgatccgg cgcccatcgc gagcacgggc caaatcacca ggacagaacg
    27601 gcatggaccg aaaacaggat gcattcgagg ttaccagagc gggtcaggtc cctggcggcc
    27661 cgggagggct cggtacagcg cagcatcggc gtgcgccctg gcgacgtggc agcccccggg
    27721 cgagcggctt cacgctcatc gagatcatgg tcgtcgtcat catcatcggc ctgctcgccg
    27781 cggtcatcgt gccgcaggtc gtcaacaagg tggatgaggc gcgggtcgcc aaggccaaac
    27841 aggacatcca gagcctggag accgccctca ccatgtttcg cctggataac tcgaaatacc
    27901 cgaccaccga tcagggactg cagtcgctgg tcaagcaacc cacggatccc tcgatccgcc
    27961 actggcgtca gggcggctat ctggagcgca tcagcaagga tccctgggga aatgactatc
    28021 agtacgtcta tcccggcaca cacggcaagg agtacgacct gtacacgctg ggtgccgatg
    28081 cgcagccggg tggcgacggc gtgaacgccg acatcggcaa ctggaacatc ggagatcagt
    28141 gaggctgcag cggcccatgc gcgctcccgg acgatccgct gggttcacgc tgatcgagat
    28201 cctggtcgtc gtcctcatca ttggcatcat ctcgctcggc gtactgctgt cggtgagcct
    28261 gaccggtcga gatcgtgagc tcgagaccga gagcgatcga ctcctcagcc tggtcaacta
    28321 tgcacgcgag caggccgagc tgcagacacg cgagtacggt gtcatcttcc acgatgatgg
    28381 ctatcagttc gtcgcctacg atcagcgccg cggccactgg cgcgaggtct acgaggatga
    28441 ggcgctgcgc cagcgcaagc tgcccctcgg gctggatttc accctctcgg tcgagtcacg
    28501 cccggtggtg ctcgttccca ccggcgaggc caagcacgac aagccgaagt cgaaagccaa
    28561 gtccctgaag gacatctcga cactggatga tgccgtgtcg aaggattcga aggattcctc
    28621 atccgggctt cggggtcagg aggactcgaa cgcgacactc gaggacgaaa agccgatcac
    28681 cccgcaaatc atgattttct ccaacggaga cctcacttcc ttcgaagtca cgctcgagcg
    28741 cgacggcggc acccgcagcg tgacgctggc tcaggacgac aagggacagg tcatcgaaaa
    28801 gccgatgatc gagcacaaga cgtgagccgc atgacgcgcg gcttcacact gatcgaggtg
    28861 ttggtcgcgc ttgtcatcgt cgcggtcggc atggctgcgc tcatgggcgc gctgagctcc
    28921 tcggctgaca ccgtcaacta catgcgtgac aagacctttg ccgaatgggt tgcgctcaac
    28981 caactcgcca acctgcgcct gcagatgcag ccaggacaag ttccggccac agggaacacc
    29041 aacggcgatc tggactatgc cggacgcagc tggcactggc gccaggaggt cgtggcgacc
    29101 caggtgcagg gcatggagcg catcgatgtg aaggtgcggc ccgcggaagt gaaggcaggc
    29161 gatgatgccg gttggttcgt cacggtcagt ggcattgtcg gtgatgcggt cgcggcaccg
    29221 gggtctgcga cgttgagctg gggaactggc gtgacccaac ccggcaacac caacggcaat
    29281 ggaacgctgc agcccggcag tactacgacc ccgggaacca cgacgcccac cacccagggt
    29341 ccgcaaccct cccccacccc aaccaagcct ttgggcggca cctcgccatg agcctcgccc
    29401 ggctgatgga gctcgacaac ggccggcact gcaagcgccg gccgggtgcc ggcagacgct
    29461 tgcgaggatt cacgctgctc gaattgctcg tcgatgttca tcgcggcggt gatcttcgcg
    29521 atgggctacg gcgcagtgaa ccaggcgctc aagagccgcg tcgagctgca ggagcaacag
    29581 gccaagctgc tgcagctgca gaccacgatg cgcgtgctcg agcaggattt cgtgcagctc
    29641 gccccgcgac cggtgcgcca gccggtcggc gacagctatc agccggcgct gcagtccaat
    29701 ccatcagcgc cgccgctgct ggcgctcact cgcggcggtt ggaccaaccc cacggggctg
    29761 cagcgacccg gattgcagcg ggtggcctac tacttcgaga acgggacgct gcggcgcgaa
    29821 ttctggaccg tcctggatcc cactcagaac agcacgacca tcaagcgcga cctgatgacg
    29881 catctgaagt cggtcacgct gcgcttcatg gatctcagcc gggtttggca accgcaatgg
    29941 ccgcccgcaa ccaacacagt gggcgcgacg aatgcggacc tgatgctgcg ctcgcgtccg
    30001 atcgcggtag aaatcacgct ggagaccgag gactggggca aggtcgtgcg cgtggtggag
    30061 atcgccgggt gagctctcga gcgcttgtcc cgacccggcg gcggtacgac agcgcggcac
    30121 ccggacggca gcgcgggatc gccatgctcg tggccattct gctcgtggcc ctcggcacca
    30181 tcctcgccgc cgcgatcgct tacgagagcg ccatgagcgc tcgccgctcg accgccacct
    30241 tctcttttga tgaggcgctg ctggtggatg agggcgccga ggccctcgca gcctatgggc
    30301 tgcgggcgat tcgccagcag gacaagacgg gcaccatcaa catggcgcag ccctgggttc
    30361 agcctgtcgg cccggtcgag gtcgtgccgg gcgtgacgct cgaagcctca ctcgaggatc
    30421 tgcagggccg cttcaacctc aattcactgg tgctgagcga tggcacgggc cagcccgacc
    30481 cccaagccgt gcagatcttc cagcggctgc tgcagctcgt gggcgtcgag cccaagtggg
    30541 ccgatttgct ggtggactgg attgatccga acaatcagcc gctgcccgat ggggcggagg
    30601 acaacgcgta tctgagccag gatccgcctt atctgacagc caacctctac atcaccagca
    30661 ccacggagct gctggcgctg cccggcttcg gcgcagaccg ttttcggaag atcgccccct
    30721 acgtcgtggc tttgccccca actgtgactc aggtcaacat ctgcacggcc tccggtgtgg
    30781 tactcgatgc cctgatggca gccgggggac agggtgagga gagccgggcc gaattcagcc
    30841 tcgacccgca gggcctggcg aagaatcgcg ccagcgccgg aatgtgtttc ccctcgctgc
    30901 aggaatacca ggcggccctc gggtcgaatg ccagcaaagc aaccaagtac gtgggcacca
    30961 attcgcatta cttccggctc tcgagcttcg tcacccttgg cactaccgaa ttcaacttgt
    31021 acagtcttct gtttcaggat gacaacctcg gaatggtcca caccgtgctg cgctcgtact
    31081 cgcccgactg aggaacgacg gattcaggct tatggctgac tggctgctat tgagaatgcc
    31141 gcgcacgccg gaggagcttg ccacctgggc cgtggtcgac ccgcgcggca accaggtcgg
    31201 cccccctcag agtggaccac tcgccctcgc cgctcctcgt gcagccggcc gacgcatctg
    31261 cgtactcgtg cccggatcgg atgtgctgct ggcagaacct gacgtgccga tgaaggccgg
    31321 caccaagctg cagcagatcg tgccgtacgc gctcgaggag cagctcgccg atgacatcga
    31381 tgacctgcac ttcgccttcg gcaagcgaat cggcgagtcc tcgcggacac cggtggcggt
    31441 ggtgcgccgg gacttgatga atgagtggct gcagacgctc aaggaaagcg gcatcgcgcc
    31501 cgacacgatg tatgccgaca gcgacctcct gccccacaat ccgggccagg ccgttgcact
    31561 gctggaggag ggcgtggtca tcgtgcgtcc gccgtcaggc tccccggtga ctttgcccac
    31621 cgaggcactg ggcgaggctc tgaatatcgc tcagcaggcc actgcggagc tgaccgccgg
    31681 cgggggccgc gggttggtcc tgtatacggg tgcggccgaa tggcatcagc attccgcgca
    31741 agtggaagcg ctgcgcgagc gcttcgatgg catcaagatc cagctgctga ccggcggtcc
    31801 gctcaccttg ttcgcccagc agctcccggt cgcctcgccc gtgaatctcc tgcagggcac
    31861 ttacgcgccc gtcacgcaga accgcgtcgg ctggcaagcc tggcgcatcg ctgcgatcct
    31921 gctcgtgtgc ctggtcggcc tgcacatcgt gggcaagacc gccgagctcg gcctcttgaa
    31981 gcggcacgag cgcgcgctcg acagctcgat cggcgagacg tttcgcgccg ccatgccagg
    32041 cgagcacagc atgctcgatg cgcgccggcg gatggaggcg cggctggcgg ctgcgcgcag
    32101 cggcggcgat tcgggcggcc tgctctctgc actggaggct ctgggtcagg cgcgcagcgt
    32161 cgccccggga gcggtcgtgc aggcactcag ctttcaccag ggcgcgctcg aactgaagct
    32221 gtcggcccct gatgccacca gcctcgacaa gatgagccag gcgctgcgcg gcaatggctg
    32281 ggatgccagc ctgacctccg gaaatgcagt gggctcagct tacgagggac ggattcagat
    32341 ccgtccacgc tagcgaggcc gcccgatgcc aacactctcg ttgcacaaca tgtccgagcg
    32401 cgagcagcgc atggtcctca tcggcgccat cctcgccgtg ctgttgctga tctttgccgt
    32461 gctggtgccg ctcgaccaca gcgtttcgaa ggcccgcgag cggatcaccc gcaagcaggc
    32521 tgaccttgcc tggatgcgca gcgtcgcccc cgagctcgcc gcagcaggcc ccgggcagct
    32581 gccggccagt aacgagtcgc tgctggtgat cgtagaccgc tcggcgcgcg agtcgggtct
    32641 cggagctgcg ctggccggga gcgatccgag cggcgccggc ggcctgcagg tgcggatgca
    32701 gaaagcctca ttcgatgcga tggtgggctg gctcgcacgc ctctcccagc agaacgggat
    32761 tcgcgtcgat ggcgcgacga tcgataatgc aggcgccccc ggcatcgtca acgcagccat
    32821 catcctgcag cgttagctgg cgagctcgac cgcggcacgc gcatgaatcg atccatctgg
    32881 ctcgccgttg ccgtcgtgtt ggtattcgcc gtgatcctga tcgcgcggct gccagccgcc
    32941 tgggtgctct cgaagccggg ctcggatctg agctgcgcgg ataccgcggg aaccatctgg
    33001 aacggcacct gcacggggat ggtcgtgcag catcagccga tcggcgatct caactgggag
    33061 gtgcacgcct ggcggctgct gtctgccaag tttgctgcca ccgtgctgct cacccgtgcg
    33121 gcgggcgtgg ctcgcggcac ggttgaagtt ggcttcggcg gccatctcac ggggcacgat
    33181 atccacgtgg atctgccgct cgatcccaga gtgatgccgc agctgccgcc aaatctttcc
    33241 ggcagcgtgc aggcgaacct cagctcgatc cagatgaagg gcaataccgt gacggccgtg
    33301 gagggtgaag tccaggcgct caatctgcag ctcggcaccg ggcccggcgc cgagccgctc
    33361 ggcagctatt cgctgacctt cagcccctcc agcggcgcac ccatcggaca gctgcgcgat
    33421 ctgggcggcc cgttgcaggt cgagggcacg ctgcgcctca cgcccgagcc gggcttcgac
    33481 ctgcaaagtc tcgtcaagcc gcgtgccaat gcgcccgagg cgctgactcg ggacatccag
    33541 ttccttggct caccggatgc ccaggggcgc cgtccgttcg ccctcgccgc gagcttttga
    33601 gcggtcgttt gcgtcgccga ctgcgctcac ccttccagct ctcgaaaagt cgcaagcagc
    33661 ggaaagtaca gcgccgctcg aaccgggtgc tctcccagat ggcgtgccag ggtcctgtag
    33721 gtctcgagct gcgggcgata gcgctgcatc tcctgctcga ggaaagcctc gagcccacca
    33781 ccttcgtgcc ggctcgtctt gaagtcgatc acccagcggt tcccgtccgc atcgaggaac
    33841 gagcggtcga ccacgacatt cacgagccgg ccggcggcga gcccggtcag tgcgagctcg
    33901 ctgcgagcct cgcgatgagt gggggcgaaa atccatcggc cccgttcatc gctcaccgtg
    33961 gccttcaaag catcgatcac cctctcagcg gcccgtggca gatcgcgctg cggcacgccg
    34021 tggcgccgaa gttgctgcaa gtaatcgccg cgctgcgctt cgattgccgc ggctgtcggc
    34081 agctgcgcgg cgcgcgagaa gctctcgagc gcggcgtgaa cgaccgtgcc gatgtgcctc
    34141 gcggtctcct gcacccagct gaactcgggc ggctcgagcg actggcggga caggggcagc
    34201 cgggagagct cggggccgcg tgcgagcgca ggcggactcc atctcgagac cagacgacgc
    34261 agatgcgtcg acgcttgtcg ctctgctgac tcgctcggcg ttgtctcggg cccggctcct
    34321 gcagcgctct gcccggcaaa ttcctcggag gtagccctgc ggagggccaa aggatccaga
    34381 gcaggccaaa gggtagccag cagcgtgccg gcgcgcgcgg tgagggtgcc attcgccttc
    34441 ggcctgggag cggccgaaag atgcaaagac tttttcgcac gcgtcgccgc gacatacagc
    34501 agtcgcgtgc gctcattggc ggcgcgctgt gccgccaatc gcttcagaaa ggtggtcact
    34561 tcgcccggcc cctcggggcc aacaggtggc acgggtgcca tgatcaggtc gctgtcccct
    34621 tgcgcgcgag gcagatcaag ccagcgcagc aacggctcgc gaccgcgatt cagatcccgg
    34681 tcgagggctg gcacgaacac gtggtcgaat tccagaccct tggcacgatg gatggtcatg
    34741 acctggaccg ggttggccga ggaggcctgc ggttgcgcgt acagatcccg caacagcgaa
    34801 tcgagatcct gcgcgccggt ccactcaaca ctcgcgactc gttggctcag cgcatcgaag
    34861 aaagcgcgcg cgtgagccag ctcactcgcc ggataagcat ctgcagcacc gagccgcagc
    34921 caggtgagct cgagccaatc caccagcgcc agcgtatgac gcgtggagag tgcagagctc
    34981 aacacatcgc gcacgcgcgc gagccgcagg caatcctccg tcgaacagtc acttaggcag
    35041 gcgggatcct cgagggcctc ccagacgagc agcggatggc gccggcccga gagagccgtc
    35101 agggcgcgca gcgacaggcc acaccaaggt gcgcgaagca aagcgagcca cgcactgcgg
    35161 tcgcccaggt ggctggttgc gcgcgtaagc gcgacgagat cgcgcacgat cggaagctcg
    35221 ctcaagggga ccaactcgat gccgaccgct tcgatgtggc gcgaggcgag ctcggccatc
    35281 agcaccgtgg cgtgtgatcg cgaggccacc aggatggcga gcgtcgcctg cgggtcccct
    35341 gctctgagct cggcgatccg ctcaccgatg gcccgcgtct cggcgtcgcg atcggcgaag
    35401 aaccgcagac gcacaggctc gcctgggcat gcagctgcgc cttgcgtgcc tggctcgctc
    35461 gcggtaaacg ccacggcgct cgcacgcaga tcatccgtct ccggatacag actgcgaaat
    35521 gccgcattcg tccaggagat gagctccggg atcgatctga agttgcgtgt cagacgcagc
    35581 gcctgcagcg gcacgctgcc gatcccttcg ttccgggcac gcagaaacag gcccacctcg
    35641 gcctcgcgga actgatagat cgactgcatc ggatcaccga ccacgaacaa cgtgcggcca
    35701 tcgccctctt cccagcctgc aaccaggcct tcgatgagct cgagctgcgc gagcgaggtg
    35761 tcctggaatt cgtccacgag gatatgacgc agagcaagtc cggttcgcag tgccagctcc
    35821 gtaggtaatc cggcctcaat cagcgccgca cgcgccgcac ccgcaacgta ggtgtagtcc
    35881 actcgaccgg ccgccgcgaa gatcgcctgc agatgggcgg ccgcaaaccg cagaacgcgc
    35941 gacaacgcct cgatggcgcc cgcatcggcc gttgccagcg tagacgacgg aagtgcggcg
    36001 acgtcgagca gcgtgaaacg caggccaggc acatcgctca tgcgctcgat cacggccgcc
    36061 agagcttcac gcgtggctgc aagctcatac ccgggccctt gcgcccgtgt gaggccactc
    36121 gacttgcgcc actcgccctg gccggtgagc gcaagacagc cgagccgctt ccactctccc
    36181 aaagtctcgg gctcggcgcc cagccttccc acaccggcga gcgccgccgc ctgggcaagc
    36241 agatcaccgg ggatgcgccg caaagtcgct acgagctgct cgcgcacgat gtggcccaga
    36301 ctctcattga tccgggcgca caaggcgtgc ggctcgtgcc cgagcacgta gcgcagccag
    36361 tggcctcggc gctgcaacat atcggcgagc agctgctcga cgttgcgcca gttgttgtcg
    36421 agccgttcga acagcagctc cacatcgagc gcgagctgcg gatcgtcctc ggcactcgcc
    36481 agggttgcgc gagccgcctc ggcgtacagc tcctcgggtc tttccgtgat cgacagcgaa
    36541 cccccggcgc gcgcggtcac ggtaagttga cttgcgagtg tgaagttgaa tgaatcgatc
    36601 gtctggatgc gcaggcggcc agggtcctgc gcgagattcc agcctcgggc cgaatcgcgc
    36661 gccagcgccg ccgcggcgag cgagcgcagc cgctgggata aagcgtccgg ggcgtgcagc
    36721 gcgggcgcgg cgctcccatc ttccatcgct ccgcgcaagg ccttcagcac gcgatcacgc
    36781 atctcggccg ccgcgcgtcg cgagaaggtg atggcaagaa tctcttccgg ctcgttgacc
    36841 tgtgcgagca accgcagcag ccgctcagtc agcaccgtgg tcttgcccga tccggcggga
    36901 gcctgcagca gcacggaccg atgggggtcc gcggcatact cgcgcgcgcg ctcatcgcag
    36961 agcccctccc cgggctcctc gatcgcaaac agatcgcctt gctcagccat cgcttggcgg
    37021 gagactgagc tcgttgagct cggagagccg ctcgagcgcg cgttctgaga tccggcagat
    37081 cccgatcgcg tggcagaagt cacaggcgcc gggcttggga tcgagaaccg cccggccggc
    37141 gagaaaatcc cgggtgaggt tttccaactt cgtgagccac tccagcgtgc gcagcgacca
    37201 ggcatcgcgc gccgccatcc cggtggcttg gacgcccttc acgcgcggca gcctgccggc
    37261 gctgtccgcg atcccatcga aacggatctc ccgcgcggtc acattgacgg tcgcggttgc
    37321 gaccgcggtg tttcgatgtg cggccagata ggcgagcaat tgtggatgcg aggggcgctc
    37381 ggaataccag tcgccgggct ggcgccggcc actcttgtag tcgaggatcg cgtgtccacc
    37441 gtcttcgagc tcatccagcc ggtcgattcg gagcctcatc ggcgcacccg cgagtgtcag
    37501 agtcgcctcg gcttccgtgg cctggacgcg aaaaggtttg cgctcgcgct cgagcgcaca
    37561 caatcccttg atcagccgaa cggtccgccg atgttcgcgt gccagcagcc gttcgcggcc
    37621 ccgggaggcc gcgccgccat agcgcggagc aagcacccgc tgcgccgcct cggcaacgca
    37681 tcgctcgatc agctcgccga gactggactc ggaatgagcg gccagcgcgc gcgagtcccg
    37741 cagggattgc cacagttttt cgagagcggc gtgcaacaac tgaccgcgca catcgagcgc
    37801 cacgccgggc tcgggagtct ccatcggttt gcttcccagc cgcagctcgg cgtaggcgcg
    37861 aaaggcacac agattctgca gctccaggct gcgcgtgccg gagggtaaga gctgcccggc
    37921 gggccaggcg agacccggat catcgatgca ttccagacgg ttgggccgat gcagacgcgc
    37981 ggcaagccat accgctgcgc gatcggtggc cgccatgtcg cccgcgcgat ccactcgatc
    38041 ggtgcccatc ggctgcgtgc cctgccccca ggcactcagc atcgggctcg gcagcagctc
    38101 cagatcacca ctgcggcggg gtacgctcaa caccaggtga ttcgttgcgg cacgccacgc
    38161 gttcatgagc gcgtgtgcct cctgcaggcg gcccgcagaa ctggcctggg gaatgccgct
    38221 cagcgtttgc agggcaagcg ggatgaaggc gtcgggctgc agcggccgcg gcagcacatc
    38281 cgcatgcaag ccggcgaccc aaataccctc gtaactgacc actgggtcca acagtgccgg
    38341 ggcgatcgtg acgagagcat cctcatccgc cggccgatag ttcgcacggg cggcgagttc
    38401 cgtcagccag tggacggcgt cctgcaacga catcgagcgg ctggcgagcg ccagttgccc
    38461 gagctcatcg agcagctcat gaaatctcac cacggtctgt tgttcgccgc ttcccctgac
    38521 gcggctgccg ggccagcccg ctgcctccag cgcggcgcga aagcgctccg accagtcacg
    38581 cggcgaggcc tgaccctcgc gcagcgcgtg agccgccaga tcgagcttgc tggcgatctc
    38641 ctgcacaggc cgcaccagct cggccggggc actcaccacg atggcccgcc agttgcgcag
    38701 atcgacgcgc gtgagactgc gctcgcgcag ccagaggtca atgcgcgcac gcgcgccctc
    38761 ctccggtgtg ctccagtacg gtgagcgcaa ccactcgctg agggcattga gctgtccagg
    38821 cgtgcctgcg agccaactca agaggtccag tgcgtgcgcc acaacgggta tccgcgccag
    38881 ccccgacccc ccttcgatga ctgcgagatt cccgatcggc tcatccgccg gtcttgccgc
    38941 tccggagtcc agccacgcgc gcgcgtccac ggtctgtcgg atcagtgtgg cgagtcgctc
    39001 tctcgagccc ggcgcaccgg gcagaatgac gaggaggcgt gcctgcgcag gaccgctcag
    39061 cagatcctgg caccagagcg caatgcgttc cagctcttca gtctcgtcgg cagccctgac
    39121 cacgcgcgga gttgccagcg atcgggccga gaccggctgc acggcgccca gggagacgag
    39181 acgcggcggc agcttcgtga agccggcgaa gaccgtcgtg cgagctcgac aggcccctga
    39241 caacgaagcc gtcaacgaat gcatccttgc ggcccccaga ctgaggcagc gcgcatcgac
    39301 cgctccctgc acctcgcaaa gaagcggcga ctcgaggccg ggggctttcg cgaagctcgc
    39361 aacgtccaga ccgaaatcgg ccgcgagcgc gctcgcctga cgcagcccat cggcgaggcc
    39421 gggacggtcg agcaactcga gcgactcggt cgcgaccgcg gtgcattgcc gccacagcag
    39481 ccattcctcc gcgggcgaga gcaggcgtgg cagcgactgg cctgctgcgg ccgcccgctc
    39541 gatttcgcgg acctgccaac cttcaaccgc gaggatctgc gggctggccc agacgtgccg
    39601 accccgcgcg agctgcgcgg cggcataggc gagacgcgcc gcatgcgccc gctggcgcga
    39661 tggaaccacg agggtcccgc ccgcctcgac tgcagccgaa atagattcct cgagtctaat
    39721 caatgaacta gtctcaaatc cttgaaacta cctgaatcgc tcgctgacct ggacgaccct
    39781 ctctgcagtg accggagtga gctgcgcact gcccgggctg cccgcgcgcg tgcgcgcccc
    39841 gctggcgtag ccgagcagca attcctcgat tctggcaaac acggtctgca gcgtcgctga
    39901 atcagggaga ttcgtgactc ggaagtgcgt gcggtacggc acgttgaagc tgacgcccgg
    39961 cgcaaccagc acgtgcttct gctccaggag gtcgagtgcg aactgctgat catcgaagcc
    40021 cggcagctca cgcgcatcca cgccgatgaa gccgtacatg gcgcccgccg gtggacgcag
    40081 atgcaggaac tgactctgtt gtacggcaga gaggatcgcc cggcgggatt catgcagccg
    40141 gcctcctgca cccaccagat cccggatgct ctgatagccc cccagtgctg tctggacggc
    40201 ccactgggcg ggaacgttgc tgcacagacg cagcgagctc agcaattcca tcgcgttcag
    40261 atagtccgtc gcggcggcga gctttccgga aaacaccgcc cagccgaccc gatagccgca
    40321 cgcgcgatag atcttcgaga ggccggaaag tgtggcgcac agcgtgtcgt tcacgagtgt
    40381 cgccatgggc acgaattcgg cgtcctcgta caccatctga tcgtagattt cgtcgctgaa
    40441 gacgatgagc ccgttgcgct cggcgagctg cacgagcgcc tcgagcaccg cgcgcggata
    40501 aacagccccg gtcggattgt tggggttgat caccacgatg gcgcgtgtgc gcgctgtgac
    40561 gaggctcgct acttcctcgg gatcgggcac gaagcggttc tcggcccgac aggggtaatg
    40621 cacggcacga cctctattga gcgtgaccgc cgcggtccag agcggataat cgggactcgg
    40681 tatcaagacc tcgtcgccat cgttcagcag ggcgcgcagc accagatcga tcagctcact
    40741 gacgccgttg ccgatgaaga cctcttcggc agtgacgccc gcgacgccgc gcgcctgctg
    40801 ctgcatgatg accgcttcac gtgccggaaa gatgcccttc tggtggcagt agccttccgc
    40861 ctccggcaga ttctcgatca ttgccagacg catcgtctcg ggtgtgcgga agccgaatgc
    40921 gcccgggtta ccgatattca gggagatgat ctcgtacccg agccgctcca gctcatgcgc
    40981 gcgccgggcg agccggcctc gaatctcata gcgaacatgc tgcaagccct cgctggggcg
    41041 aaagccgatc tcggttttca tggcgcaaga atagcgcacg aggccacgcg cagcgctcaa
    41101 tcgcctgcat cggaaagcgc cgcaagctcc cgccaccgat ggcaggcgac tctctgccgg
    41161 ggtgcgccgc cctcctccca ggcgggaagg cgctcgcggc agatctcgat ggcgtgcgcg
    41221 catcgtgtgc ggtacacgca gccgctcggc agggcgagtt gtgaaggtgg ctcgcccgca
    41281 aggatgcgca ggagccgccc tggctgcaca gccggatccg gggtcggcgc ggcagccatg
    41341 agctcgcgtg tgtagggatg cagcgcacga ccgaagagcg atcctgcccg ccccagctcc
    41401 atcatgcgac ccagatacaa caccagcacg caatcacaca gttgcttcac gaccgcgagg
    41461 ttatggctga tgaacaggat cgaaaggccc tcgtcacgct tcagctcggc gagcagccgc
    41521 aggatctggg cctgcacact gacatcgagt gcgctgaccg cctcgtcgca cacgagcacg
    41581 cgcgggcgca gaacgagcgc gcgcgcaatg ccgacgcgct gacactggcc gccactgagc
    41641 tcgtggggat atcggttcag cagctcggca gcgagtccga ctcgcagcat cgcctcatgc
    41701 accgtctgca gccgctgcgc agcgcccatc tccggccgat gaacttgcag cgcctcagcg
    41761 atgctctcgc ccgccgtcct gcgtggatcg aggctagcga gcggatcctg gaagatgatc
    41821 tgcacctcgc tgcgcagcgg ccggagcact gcccccggaa gactctcgat cgagcgtccg
    41881 agccacgcaa tgctgcctga ctgcggccgc agcaatctca gtactgcacg cgccagcgta
    41941 gacttgccgg aacctgactc gccgacgatg cccaaggcct ccccggcatg cagctgtacg
    42001 tcaacgccca gcagcgcatg cagcgtggct gcgcgtccga gccgactggt acggacctcg
    42061 aactgcacac gcacgtccgc tgcctgcaac gtgaccgggc cctcggaatc tgtaccggcg
    42121 ggacctgcgg catccagccg cggcaccgcc ttgagaagct cctgcgtgta gggatgttgg
    42181 ggtcgcgtca aaattgtgtg cgccgaatcg atctcgagga ggctgcccgc ctgcatgacg
    42241 gctacacggt cggccaaccc ggccacggcg cccagatcgt gcgtaatcaa caccatcgag
    42301 aggccgcgct tgcgcttcaa ctcggcgagc agcgacagaa tctgtgcctg aatggtcaca
    42361 tcgagcgcag tggtcggctc atcggcgatg agcagcgaag gctcacaggc cagtgcgatc
    42421 gcgatcatga cccgttgttg catgcccccc gagagctcat gcgggaactg gtgcagccgc
    42481 cgcgccgcgt cggtgacctg tgcctgctcg agcagctcga gcgcacggcc gcgcgcgcaa
    42541 cgccaggaga gctggcggtg ccggaccagc ggctcggcga tctgctcgcc cacacgcaaa
    42601 tggggcgtga gcgaggtcat cgggtcctgg aagatcatcc cgatgcgtgc accgcgaatc
    42661 cgatcgagct caacgcgtga cgaagcgatg agatcgcgac cggcgaacca ggcatgccct
    42721 cgggcgcgcc cgttttcggc caggagcccg aggatggcga gaaatgcctg gctcttgccg
    42781 gcacccgact cgccgaccac gctcagacat tcacctggag cgaccgagag agacagtgag
    42841 cgcaccgcgt gcacctcgct ctgcggggtc tggaagctca cctcg
//
LOCUS       DASRHN010000099        15299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_364009, whole genome shotgun sequence.
ACCESSION   DASRHN010000099 DASRHN010000000
VERSION     DASRHN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15299
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364009"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3802
                     /locus_tag="VFB37_03795"
     CDS             <1..3802
                     /locus_tag="VFB37_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO21603.1"
                     /translation="QAIVPGAAGTWKDLTDNVNSMASNLTGQVRNIAEVATAIARGDL
                     SRKITVDVRGEILQLKETINTMVDQLNSFAAEVTRVAREVGTEGKLGGQAIVPGVAGT
                     WKDLTDNVNSMARNLTGQVRNIAEVSTAIARGDLSRKITVDVKGEILQLKETLNTMVD
                     QLNGFAAEVTRLAREVGTEGKLGGQAVVPGVAGTWKDLTDNVNVMANNLTNQVRGIVK
                     VVTAVANGDLRQRLTVESKGEVNALADTINSMTDTLAIFADQVTSVAREVGVEGRLGG
                     QAHVPGAAGTWKDLTANVNLLAANLTNQVRAIAEVATAVTKGDLTRSIQVEARGEVSE
                     LKDNINAMIGNLRATTERNTEQDWLKTNLAKFSRMMQGQRDLESVGKLLLSELAPLVN
                     AQQGLVYVMEGTGSGSGLKELASYADEGGVNHDSRRFAVGQGLVGQAAADRQRLLVTD
                     IPANSIQVRGGLLSARPRSVIVLPVLYEGHVKAVLELASLGEFTASHLAFLEQLTGSI
                     IGVVLNTIEATMRTEGLLKQSQQLAGELQAQQAELQQTNEELANKAKLLAEQNVEVER
                     KNQEIELARRALEEKAAELALTSRYKSEFLANMSHELRTPLNSILILGQQLSENADGN
                     LSGRQVEFAKTIHGAGTDLLNLISDILDLSKIESGTVTVECEDLLFTHLRETIERNFR
                     HEADARKLSFTTDFDSRLGRAITTDSKRLLQVLKNLLSNAFKFTDRGGVRLHVGVANA
                     GWTTEHPILSQAPTVIELSVSDTGIGIPPEKQKIIFEAFQQADAGTARKYGGTGLGLA
                     ISRELAHLLGGEIRLSSIHGSGSTFTLYLPLSYLGPAYNKAMAIGQSPASVAASYQPA
                     VLPVVRQEEISDDRDQVQPDDAVLMVVEDDPHYARVLLSLARDRGFKVLLAKTGADAL
                     SLAAKYRPTAVSLDVFLPDMLGWTVLNQLKRNPETRHIPVQILTVEDERQYGLERGAF
                     SFVTKTVTSDALEQAFDRLKSFTTKRLRRLLVVEDDPAEQMSVKELLGHDDVEIASAA
                     TGAKALEAMRSTPFDCVVLDLRLPDMSGFDLLAEVQQDPALRETPIIVFTGRELSESE
                     ESELRKKAKSIVLKGVRSPERLLDETALFLHRVIADLPASKQHMIETLHESDEPLNGR
                     KVLVVDDDIRNIFALNSLLERHHMQVITATNGHEAINLVENTEDLSLVLMDVMMPEMD
                     GYETMRRIRSNHQFRMLPIIALTAKAMKGDREKCLEAGASDYVAKPVNTDQLLSLVRM
                     WLHR"
     gene            3860..5644
                     /locus_tag="VFB37_03800"
     CDS             3860..5644
                     /locus_tag="VFB37_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO21604.1"
                     /translation="MSAAERVSILLVDDQPARLLTYESILSELGQHLVCARSGLEALD
                     RVMRHEFAVVLLDVSMPDMDGFEVARLIHEHPRFEKTPIIFVTGVHVSELDRLKGYKL
                     GAVDYVSIPVVPEILRSKVSVLVELFLKRRELVELNRTLALANAQLAEANTTLQAEKT
                     RELEAINVHLQRVNTELEQANRTLQSEIGERTRVETALKEADRHKDEFLAMLAHELRN
                     PLAPILNAVQLMRLRPIVDPQLTWCREVIGRQLGHLTRLVDDLLDVSRITRGKINLTR
                     EKVELAVIAARAVETVQPLIAEREHQLTVTLPEEPVRVYGDPLRLTQALGNVLGNAAK
                     YTDRGGRIELVARRQGEHAEVRISDSGIGIPAALMPNIFNLFTQINQQPDRPQSGLGI
                     GLSLVRRLLEMHGGSITAFSDGEGQGSEFVIRLPAVAEMTDTSSATQPAPAQPPEDPT
                     MTTPLRRRILVADDNMDALESLAALLQLGGHEVFSAHNGAVALESAERHLPELALLDI
                     GMPMLDGYEVARRIRAQPWGDRITLVALTGWGQDSDRHRSGEAGFDSHLVKPLDLDKL
                     TDLLQRLPLRVAKDGDGRNVQDGSTSAA"
     gene            5656..6627
                     /locus_tag="VFB37_03805"
     CDS             5656..6627
                     /locus_tag="VFB37_03805"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO21605.1"
                     /translation="MRELTKIIVTELLLGLAGILIMGAVIAWYAGGELIDPVPRYIGH
                     PPRALSVANASFVNGSGTLVHGWVSHGKAGQGVILLLHGLHGDRRDMVPRAEFLSRLG
                     YSVLLIDFQAHGESTGRHVTFGFLESRDVVGAIQYLRYTLPDEPIGVIGASLGADAFA
                     LARSRLPVDAVILESMYASVKEAVESRLRVHVGLFAPAFASLMAVQVRTRLGISHDSQ
                     EIMEGMKHLDAPLLMLSSTDDPNVPLTDAQALFGAAAEPKEFWAVRGAGRGDLYEFEK
                     AEYERRVAAFLRDHLKPRAKSHSDSQEEDSPLEDSRGAGRPQSLKTG"
     gene            complement(6672..7484)
                     /locus_tag="VFB37_03810"
     CDS             complement(6672..7484)
                     /locus_tag="VFB37_03810"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HZO21606.1"
                     /translation="MLSWILQPGTAGAQTPSPLQEWQYSGGIVLARLFEPHLPEWRTV
                     AGVATEVQPVYDGSNAYRVQPGPVFNIRYKDIAFLSVGEGLGVNLLRGDHYQAGVAVA
                     YDLGRKMSDDYTHLRGLGDIAPAPAFKAFATYVLAKKFPLVLRADVRQIVGGANGMVG
                     DLEAYLPLPGSSRKFVMFAGPSITYADHRYLQHVFGVDRAQSLASGYPEFGVHGGENA
                     VGVGFSATRFITKHWLVNLDAAVNHLRGSAEESPIIQQRRQRVLALSTEYMW"
     gene            complement(7646..7852)
                     /locus_tag="VFB37_03815"
     CDS             complement(7646..7852)
                     /locus_tag="VFB37_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZO21607.1"
                     /translation="MNNDRTAGNWHQIKGKVKEQWGKLTDDDLKQLEGQSEQLAGKLQ
                     ERYGLARDEAERQAKEFRSRNNWH"
     gene            8083..8601
                     /locus_tag="VFB37_03820"
     CDS             8083..8601
                     /locus_tag="VFB37_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZO21608.1"
                     /translation="MTVAIAPCAGAAEKLVLEASKQSVRASAVRKNATTLRSTRTTVR
                     STRKPTADFSGKRRLGKASFYADWFAGRKMANGAPMNPHGDNAASRTLPLGTVAKVTN
                     LETGKSAVVSIQDRGPYVDGRIVDLSPTTARKIGLTQRQGIVRVAVAPIVVPLPNGEV
                     KPGVAAHEAEGG"
     gene            8768..9208
                     /locus_tag="VFB37_03825"
     CDS             8768..9208
                     /locus_tag="VFB37_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21609.1"
                     /translation="MAIARQTFYGSALRILVICAVVACIYYLWAGQPTVSTAIAIGIA
                     CVILARLVIRARLFPNPFGETDRDYSVRPVLWGIIKGGVCLSAAFVWGIGCVLAMRYL
                     VIPDTPLVEYGLMGAPFVLLILAGAWFLIASGIRAVYGHDRRGY"
     gene            complement(9441..10082)
                     /locus_tag="VFB37_03830"
     CDS             complement(9441..10082)
                     /locus_tag="VFB37_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21610.1"
                     /translation="MEGLGLHGWENFYVIVGSSAGALTGLTFIVITISSSDGSGGFSN
                     SPVARLMGMRAFITPIAVHFCSALWVSFLLSVPVQTVLSLTVCLGVTGLAGAVYCAQI
                     TAWMLKTRVHYKPFLSDWIWNVILPGTSYLALLVAALLLSGHTLAAMLVVAAASVLML
                     FIGIHNAWDVVAWITTERHAYGRRRQGDPGRVEDDSGGASEDANKPKRSGPSR"
     gene            10284..11765
                     /locus_tag="VFB37_03835"
     CDS             10284..11765
                     /locus_tag="VFB37_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO21611.1"
                     /translation="MLPRYALLLVFSSLGSIALSAPGPADLQIGLHLADCTAGSAHLR
                     ARCGTFVVYEDRAAASGRKVEIPLIVIPAKQPTHKGIFWNPGGPGAGAVGFAGQIADG
                     EVAKELMALHEHYDIVLANNRGVGGSSSQQCDLAPRDHPEYYFLQIWPDALLKQCRAR
                     LGANANLSLYSTMVSADDMEDMRAALRYDRIVLDGGSYGTYFFLAYLRQHPEHVESAI
                     LDGVAPPGLLTIPLEDAAGAQLAMDHLIAACAADATCHTSFPQLAQHFAELVHRFAGG
                     PVRITIENPATKRLQAVFLSKEVFADRLRQTLYSAGSAAYVPYILERAYLGDYVPLGR
                     MIDTTTHQLGSLVEVGLNLSVTCAEDIPFISEAEVRSSSAGSFEGDVRVRAQQRACKI
                     WNVSPVPASFNAPVRSSVPVLMVSGSSDPASPAEYGTRELAYLSNARQALVRGAGHTE
                     ETPCTDRLKVMLVLKASVSELDTASCTSAFERPPFATSMAGFG"
     gene            11851..12117
                     /locus_tag="VFB37_03840"
     CDS             11851..12117
                     /locus_tag="VFB37_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21612.1"
                     /translation="MIRLFRLWRLGRQDLRLLWYALHHPSRPAWLWPAALVMVAYALD
                     PLNLAIPVLGVVDDFVLLPLALHWLLKWLPADIRLGFARPSLVR"
     gene            complement(12134..12820)
                     /locus_tag="VFB37_03845"
     CDS             complement(12134..12820)
                     /locus_tag="VFB37_03845"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HZO21613.1"
                     /translation="MVVRSSTKPERTTAQKLLWNHTRVWLGIVLGLLLFCLLPRAWSA
                     IERALTAWNATVLFMLLATWIKLLRVNAQQLRAYYEEEDPTAPVILVAVVAAAVVSVM
                     AIVALLSTVKHMEPAARVAHILLATLTILDCWMLVPTMFTLHYADLFYSADEANRPLR
                     FPETKQPVFWDFVYFSFTIAAACQTADVATADRNIRRTVVVHSLISFLFNLAILGFAI
                     NVTAGLLSGD"
     gene            complement(13447..13848)
                     /locus_tag="VFB37_03850"
     CDS             complement(13447..13848)
                     /locus_tag="VFB37_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZO21614.1"
                     /translation="MAETNTRTATCSCGQLRVVVQGEPARVGVCHCHACQRRTGSAFG
                     VQARWPRDKVTTEGETRIWRRIGDDGTALDFRFCPHCGSTVWYTQDVAPELIAVAVGA
                     FGDPGFPPPARSVYEVYRHPWVQFPKELERL"
     gene            complement(13855..14079)
                     /locus_tag="VFB37_03855"
     CDS             complement(13855..14079)
                     /locus_tag="VFB37_03855"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HZO21615.1"
                     /translation="MAADLILQSELSCPICGHRQLATMPTDACIYFYPCVGCGTTLRP
                     QPGDCCVFCSYGNIPCPPVQAAQPCCGGIP"
     gene            14310..14639
                     /locus_tag="VFB37_03860"
     CDS             14310..14639
                     /locus_tag="VFB37_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO21616.1"
                     /translation="MESKSAVDALSALAQETRLQIYRLLVQAGPDGLPAGDVAARVGI
                     PANTLSFHLKALSHAGLVTSRQAGRFVYYAANYEQMTTLLGFLTENCCGGRSCLPAPA
                     PRRRKVS"
     gene            14636..15127
                     /locus_tag="VFB37_03865"
     CDS             14636..15127
                     /locus_tag="VFB37_03865"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HZO21617.1"
                     /translation="MKRFHVHVSVSDLTESVSFYSQLFGVEPTVHKADYAKWMLEDPR
                     VNFAISSQRQPVGLNHLGFQVESDEELHRMRTQLAAADARMIQEQAQPCCYSRADKYW
                     VTDPTGIAWETFHTLGSIPVYGEDTSVFEHGASSVPAPSGTAKESCCIPAERSALEPC
                     CSK"
BASE COUNT         2897 a         4821 c         4914 g         2667 t
ORIGIN      
        1 ccaggcgatc gtgccgggcg cggccggtac gtggaaggac ttgacggaca acgtcaactc
       61 catggccagc aatctcaccg gccaggtccg taatatcgcc gaggtggcca cggccattgc
      121 gcgcggcgac ctgtcacgca agatcacggt ggacgtgcgc ggcgagatcc tgcagctgaa
      181 ggagaccatc aacacgatgg tggaccagct gaacagcttc gctgccgaag tgacgcgcgt
      241 ggcgcgcgaa gtcggcactg agggcaagct cggtggccag gccatcgtcc ctggcgtcgc
      301 cggcacctgg aaggacctga cggacaacgt gaactcgatg gcgcgcaacc tgaccggcca
      361 ggtgcgcaac atcgcggaag tctccaccgc gatcgcccgg ggcgacctgt cgcgcaagat
      421 cactgtggat gtgaagggag agatcctgca gctgaaggag actctcaaca cgatggtcga
      481 ccagctcaac ggcttcgcgg ccgaagtgac gcgcctggcg cgcgaggtcg gcacggaagg
      541 caagctcggt ggccaggcgg tggtcccggg tgtcgccggc acctggaagg acctcacgga
      601 caacgtgaac gtgatggcga acaacctcac gaaccaggtg cgcggcatcg tgaaggtcgt
      661 gaccgcagtc gccaatggtg acctgcggca gcgcctcacg gtggagtcca agggcgaggt
      721 gaacgccctg gccgacacga tcaacagcat gaccgacacg ctggcgatct tcgccgatca
      781 ggtgaccagc gtggcgcgcg aagtgggtgt cgaaggccgc ctgggcggtc aggcccacgt
      841 gcccggcgcg gcgggcacct ggaaggacct gaccgccaac gtgaatctgc tggcggccaa
      901 cctgacaaac caggtgcgtg ccatcgccga agtggcgacg gccgtgacca agggcgattt
      961 gacccgctcg atccaggtgg aagcgcgcgg cgaggtgtcc gagctcaagg acaacatcaa
     1021 tgccatgatc ggcaacctgc gcgccacgac ggagcgcaac actgagcagg actggctgaa
     1081 gaccaatctc gcgaaattca gccgcatgat gcagggtcag cgggacctgg agagcgtcgg
     1141 caagctgctg ctctcggaac tggcgccgct ggtgaatgcg cagcagggcc tcgtctacgt
     1201 catggaaggc accggcagcg gatccggcct caaggagctc gccagctacg ccgatgaggg
     1261 cggtgtcaat cacgactcgc ggcgcttcgc tgtcggccag gggctggtcg gccaggcggc
     1321 cgctgatcgc caacgtctgc tggtgaccga tattccggca aactccatcc aggtgcgcgg
     1381 tggcctgctg agtgccaggc ctcgcagcgt gatcgtcctg ccggtgctct acgaggggca
     1441 tgtcaaagcg gtcctcgagc tggcctcgct cggggaattc accgcttcgc acctcgcctt
     1501 tcttgagcag ctcacgggca gcatcatcgg cgtggtgctg aacacgatcg aagcgacgat
     1561 gcgcaccgaa ggcctgctga agcagtcgca gcagctggcc ggcgagctgc aggcgcagca
     1621 ggccgagctg cagcagacca acgaagagct cgccaacaag gcgaagctgc tggccgagca
     1681 gaacgtggag gtggagcgca agaaccagga gatcgagctg gcgcggcgcg cgctcgagga
     1741 gaaggccgcg gagctggcgc tcacctcgcg ctacaagtcc gaattcctgg ccaacatgtc
     1801 gcacgagctg cgtacgccgc tcaacagcat cctgatcctg ggtcagcagc tatcggagaa
     1861 tgcggacggc aacctcagcg gccgccaggt cgagtttgcc aagaccatcc acggagccgg
     1921 taccgacctg ctcaatctga tcagcgacat cctcgatctg tcgaagatcg agtcgggcac
     1981 cgtgacggtg gaatgcgagg atctgctctt cacgcacctg cgcgagacga tcgagcgtaa
     2041 cttccgtcac gaggccgatg cgcgcaagct gtcgtttacc accgatttcg acagccgtct
     2101 cggccgggcg atcaccaccg actccaagcg cctgctgcag gtactgaaga acctgctttc
     2161 caacgccttc aagttcaccg accggggcgg agtgcggctg cacgtcgggg tggcgaacgc
     2221 tggatggacg acagagcacc ccatcctgag ccaggccccg acggtcatcg agctctcggt
     2281 gagcgatacc gggatcggga ttccgccgga aaagcagaag attatcttcg aggccttcca
     2341 gcaggccgat gccggcacgg cccgcaaata cggcggcacc ggtctcggcc tggccatcag
     2401 tcgcgagctg gcgcatctgc tgggcggaga gatcaggctc agcagcatcc atggcagcgg
     2461 cagcaccttc acgctgtacc tgccactgag ctacctgggc cccgcctaca acaaggccat
     2521 ggcgattggt cagtccccgg cttcggtcgc cgcttcctat cagccggcgg tgctgccggt
     2581 ggttcgccag gaagagatca gcgatgaccg ggatcaggtc cagcccgatg atgccgtgct
     2641 catggtcgtg gaggacgatc cgcactatgc gcgcgtcctg ctgagccttg cgcgcgatcg
     2701 aggcttcaag gtgctgctgg cgaagaccgg cgcggatgcc ctgtcgctcg cggccaaata
     2761 ccgcccgacg gccgtgtcgc tcgatgtctt cctgcccgac atgctcggct ggaccgtgct
     2821 caatcagctc aagcgcaacc cggagacccg gcacatcccc gtccagatcc tcaccgtcga
     2881 agacgagcgc cagtatgggc tcgagcgcgg cgcattctca ttcgtcacca agaccgtcac
     2941 gagcgatgct ctggagcagg ctttcgaccg gctgaagagc ttcacgacca agcggctgcg
     3001 acggctgctg gtggtggaag acgatcccgc cgagcagatg agcgtgaagg agctgttggg
     3061 tcacgacgat gtggagatcg ccagcgccgc aacgggtgcg aaggcgctcg aagcgatgcg
     3121 cagcacgccc ttcgactgcg tggtcctcga tctgcgcctg ccggacatgt ccggtttcga
     3181 tctactggcg gaggtccagc aggatccggc actgcgcgag acgcccatca tcgtcttcac
     3241 cgggcgggag ctgtccgaga gcgaggagtc cgagctgcgc aagaaggcca agagcatcgt
     3301 gctcaagggc gtgcgctcgc ccgagcggct gctggatgag actgccttgt tcctgcaccg
     3361 cgtgatcgcg gacctgccag cctcgaagca gcacatgatc gagacgctgc acgagagcga
     3421 tgagccgctg aacggccgca aggtgctggt cgtcgatgac gatatccgca acatcttcgc
     3481 gctcaacagc ctgctcgagc ggcatcacat gcaggtcatc accgcgacca atggtcatga
     3541 ggccatcaat ctcgtggaga acaccgagga tctgtcgctg gtgctgatgg atgtcatgat
     3601 gcccgagatg gacggctacg agaccatgcg caggatccgc agcaaccatc aattccgcat
     3661 gctgcccatc atcgcgctca cggcgaaggc gatgaaaggg gaccgggaga agtgcctgga
     3721 ggcgggcgcc tcggattacg tggccaagcc tgtgaacacc gatcagcttc tgtcactcgt
     3781 acgcatgtgg ctgcaccggt aaacgcgtga agacacgtat catcgtgatt cagcgctaac
     3841 ggtcagagtc tctaacgaca tgagcgctgc tgagagagtg agcattctgc tggtggatga
     3901 ccagccggcg cgtctgctga cctacgagtc catcctgtcc gagctcggac agcacctggt
     3961 atgcgcccgc tcgggcctcg aggccctgga ccgggtcatg cgccacgagt ttgccgtcgt
     4021 gctgctcgat gtcagcatgc cggacatgga cggcttcgag gtcgcgcggc tgattcacga
     4081 gcatccgcgc ttcgagaaga cgccgatcat cttcgtcacc ggcgtgcacg tgagcgagct
     4141 ggaccggctg aaaggctaca agctcggcgc cgtcgactat gtttccatcc cggtcgtgcc
     4201 cgagatcctg cgcagcaagg tctcggtgct cgtcgagctg ttcctcaagc ggcgggagct
     4261 ggtcgagctg aaccggaccc tggcgctggc caatgctcag ctcgccgagg ccaacaccac
     4321 gttgcaggcg gagaagaccc gcgagctcga ggcgatcaac gttcacctgc agcgcgtcaa
     4381 cacggagctc gagcaggcga accggacgct gcagagcgag atcggcgaga gaacccgggt
     4441 ggagacggcg ctgaaggaag cggaccggca caaggatgag tttttggcga tgctggccca
     4501 cgagctgcgt aatccgctcg cccccatcct gaatgcggtg cagctgatgc ggctgcgccc
     4561 gatcgtggac ccgcaactga cctggtgccg ggaggtcatc gggcgccagc tcgggcatct
     4621 cacccggctc gtcgatgatc tgctggatgt ctcacggatc acgcgcggca agatcaacct
     4681 gacccgggag aaggtggaac tggcggtgat cgccgcccgg gcggtggaga cggtgcaacc
     4741 tctgatcgcc gagcgcgagc atcagctcac ggtcacgttg cccgaggaac ccgtgcgcgt
     4801 ctatggagac ccgctgcggc tcacccaggc tctcggcaac gtactcggca acgccgcgaa
     4861 atataccgat cggggcggcc ggatcgagct cgttgcccgg cgccagggcg agcacgccga
     4921 agtgcgcatc agcgactccg gaatcggcat ccccgccgcg ctgatgccca acatcttcaa
     4981 cctcttcacc cagatcaacc agcagccgga ccggccgcag agcgggcttg gcatcggctt
     5041 gtcgctcgtc aggcggctgc tggagatgca tggtggctcg atcaccgcct tcagcgacgg
     5101 ggagggacag ggaagcgagt tcgtgatccg ccttccggcc gtagcggaaa tgaccgacac
     5161 ttcgagcgcc acgcaacccg cgcccgcgca gccacccgag gacccaacga tgacgacccc
     5221 actgcgacgg cggatcctgg tcgccgatga caacatggat gccctggaga gtcttgccgc
     5281 gctgctgcag ctcggcggcc acgaggtgtt cagcgcgcac aacggggcgg ttgcgctcga
     5341 gtccgcggag cggcatctgc cggagctcgc gctgctcgac atcggcatgc caatgctcga
     5401 tgggtacgaa gttgcgcggc gcattcgcgc acagccttgg ggtgaccgga taaccctggt
     5461 ggccttgacg ggctggggtc aggactccga tcggcatcgc tcgggagaag caggcttcga
     5521 cagtcatctg gtcaagcccc tcgacctgga caagctcacc gatctgttgc agcggcttcc
     5581 gctgcgcgtc gcgaaggatg gggacggccg aaacgttcag gacgggagca cctcggccgc
     5641 ctgagccgga gcggcatgcg cgagctgacc aaaatcatcg tcaccgagct gctgctcggt
     5701 ctggcgggga ttctcatcat gggtgcagtg attgcctggt acgccggcgg agagctcatc
     5761 gatcccgtgc cgcgttatat cgggcatccg ccgcgcgccc tgagtgtcgc gaatgccagc
     5821 ttcgtgaatg gctcgggtac cctcgtgcat ggttgggtat cccatggcaa ggccgggcag
     5881 ggcgtgatcc tgctgctgca cggactgcac ggtgatcggc gcgacatggt tccgcgagcc
     5941 gagtttctga gccggctcgg atattccgtg ctcttgatcg acttccaggc tcatggtgaa
     6001 agcaccggcc ggcacgtgac cttcggattt ctcgagtcac gcgatgtcgt aggggcgatc
     6061 cagtatctgc gctacacgct cccggacgag ccgatcggcg tcatcggagc ttcgctgggg
     6121 gcggatgcgt ttgctcttgc gaggagccgt ctgccggtgg acgccgtgat cctcgagtcc
     6181 atgtacgctt ccgtgaagga agctgtggaa agtcgtctgc gcgtgcacgt gggtctattc
     6241 gcgcccgcgt ttgcttcgct catggcggtg caggtgcgca cccgcctggg gatctcgcac
     6301 gattctcagg aaatcatgga gggcatgaag cacctcgacg cgccgctgct gatgttgagc
     6361 agcaccgatg acccgaacgt cccgctgacg gatgcacagg cactcttcgg agcggccgcc
     6421 gagcccaagg agttctgggc ggtacggggg gcggggcgtg gggatctgta tgagtttgaa
     6481 aaagcggagt acgagcgacg cgtcgccgct tttctacgcg atcacctcaa gccccgcgcc
     6541 aagtcacact cggattcgca agaggaggat tcaccgctcg aggacagccg gggcgcgggc
     6601 cggccccaga gcctgaagac gggctgaact cgccggccga ggggggcgtg tggcccagcc
     6661 cggcggcgct gctaccacat gtactccgtg gacagcgcaa ggactcgctg gcgcctctgt
     6721 tgaatgatcg ggctctcctc ggcgctcccg cgcaggtgat tgaccgccgc atcgagattc
     6781 accagccagt gcttggtgat gaagcgcgtg gcgctaaaac ccacaccgac cgcattctct
     6841 ccaccgtgga cgccaaactc cgggtatccg gaggccagcg attgagcacg atcgactccg
     6901 aaaacgtgct gcagataccg gtgatcggca taggtgatgg aggggccggc aaacatcacg
     6961 aacttcctgg agctgccggg aaggggcagg taggcctcga gatcgcccac catgccgttc
     7021 gctccgccga cgatctggcg cacgtcggcg cgcaggacga gcgggaattt cttcgctagc
     7081 acatacgttg cgaatgcctt gaacgcgggc gccggcgcga tatccccgag cccacgcagg
     7141 tgcgtgtagt catccgacat cttgcgcccg agatcgtagg cgacggcgac ccctgcctga
     7201 tagtgatcgc cgcgcagtag attcacaccg agcccctctc cgaccgagag gaatgcgatg
     7261 tccttgtacc gtatgttgaa aacaggcccg ggctggacgc ggtacgcgtt cgacccgtcg
     7321 tataccggct gcacctcggt ggccacaccc gccacggtgc gccactccgg caggtgcggc
     7381 tcgaacagcc gtgccagcac gattccgccg gaatactgcc actcctgcag gggagaaggg
     7441 gtctgcgctc cggccgtccc aggctggaga atccagctga gcaagagagc gcaggcagct
     7501 cgaatcgtca gacgagctgc gcggcagtac cgctcagggt ttgagccaac gagtggacgg
     7561 agccagggca gagacaagtt agacaggccg gcccgggcga aggaggtgcg ggcagacgtg
     7621 aaagccgccc gcatgattgg gggcgtcaat gccagttgtt gcgactgcga aactccttcg
     7681 cctgacgttc agcttcgtca cgggcgagac catagcgctc ctgcagcttg ccggcgagct
     7741 gctcgctctg gccctcgagc tgtttgaggt catcgtccgt cagcttgccc cactgttcct
     7801 tcactttgcc cttgatctga tgccagttgc cggcggtgcg atcattgttc acgggtatcc
     7861 ctccagaaaa tagcgacagc aaaggcgtag caagctccgg gcctgcacgc tgcgacggcg
     7921 attgccacct ccagcaacgc ctcgcccctg ccacgcctcc gcctaggggt atcccaccgg
     7981 aaccgaatca ggggctggtc ggttttctat aggagcaggc agccaaagga gctcactgtg
     8041 aaccaactgg ccgcggcgat gtttgtaggg gtcggggcag ccatgactgt tgcgatagct
     8101 ccctgtgccg gtgcagcgga gaaattggtg ttggaggcgt cgaagcaatc agtaagagcc
     8161 tcggcggtgc gtaagaatgc gactacactc aggagtaccc ggactacagt caggagtact
     8221 cgcaaaccca ccgcagattt ctccggcaag agacgcctgg gcaaggcgtc cttctatgcg
     8281 gactggtttg ctggcaggaa gatggccaac ggtgctccga tgaatccgca cggtgacaac
     8341 gcggccagcc gcacgttgcc cctcggtacg gtcgcaaaag tgaccaacct cgagaccggc
     8401 aagagcgccg tcgtgtccat ccaggaccgc ggcccgtacg tggatgggcg catcgtcgat
     8461 ctgtcaccca cgacggcccg caagatcgga ctaacacaac gtcagggcat cgtgcgggtc
     8521 gcggttgcgc cgatcgtcgt tccgctcccc aatggcgagg tcaaaccggg cgtcgcggcc
     8581 cacgaggctg aaggaggctg atcgcctggc tttgcggcgc cgcgccacgc gccgcccagt
     8641 tcgcccgcac gacggcattt cgcgacgact tccctggggc gattgcgccg gttaccatca
     8701 cccggggccg gttggcccgc atcaaagcgc cgcaagccgc ttttgacaca ggggagatga
     8761 gccggacatg gcgatcgctc gtcaaacgtt ctacggcagt gcgctgcgca ttctggtcat
     8821 ctgtgcggtg gtggcttgca tctactatct gtgggccggg caacccactg tctcgaccgc
     8881 catcgcgatc ggcatcgcat gtgtgatcct cgctcgactg gtcatcaggg ccaggctctt
     8941 tcccaacccc ttcggtgaga ccgatcgcga ctacagcgtg cgacctgtcc tgtggggaat
     9001 catcaaaggc ggcgtttgcc tcagcgccgc tttcgtatgg ggcataggct gcgttctcgc
     9061 gatgcgttat ctcgtgatcc ctgacacgcc attggttgag tatgggctca tgggcgcccc
     9121 tttcgtgctg ctgatcctcg caggcgcgtg gttcctcatt gccagcggca tcagggcagt
     9181 ctacggacac gaccgaaggg ggtattaact gacccaggcg cggccctggc gggcgccgga
     9241 tgaccaggca acgcacagac cgccgccagc tccgccggca gaaccagaac tgcacggcga
     9301 tccatgcgcg gtgggccacc gtgccccaag gtcacgagcg cccacgcgga tacgttaggc
     9361 cccaacggcg agcgatgatt cgtcgcagat caaatggtga ttgcccggcg gcgatcgcca
     9421 gcctatccgg tccccagcga tcagcgcgag gggcccgaac gtttaggttt gttcgcatcc
     9481 tcgctcgcac cgcccgaatc atcctcaact cggcccggat cgccctggcg acgacgaccg
     9541 taagcatgtc tctcggtcgt aatccatgcg accacgtccc aggcgttgtg aatgccgatg
     9601 aacagcatca acacagaagc ggcggcgacc acaagcatcg cggctagcgt gtgaccgctc
     9661 agcagcaggg ccgccaccaa cagggccagg tagctcgtgc ccggcaggat gacattccat
     9721 atccagtcgc tgagaaaggg cttgtagtgc acccgggtct tcaacatcca ggcggtgatc
     9781 tgcgcgcagt acacggcgcc ggccaagccc gtgacgccaa ggcaaaccgt caggctgagc
     9841 acggtctgca ccggcacact gagcaggaac gatacccaga gcgccgagca gaaatggacc
     9901 gcaatagggg taatgaatgc ccgcatcccc atcagccgcg cgaccggcga gttgctgaag
     9961 ccaccgctgc catcggatga ggagatggtg atgacgatga aggtcaggcc cgtcagcgcg
    10021 ccggccgatg agccgacgat cacgtagaaa ttctcccacc cgtgcaggcc taatccttcc
    10081 atgcggcgat tgtatgtgaa gcgcaccgag cgtggcgcgc ggaccgcgac cgcctggcca
    10141 gtcggaccgc cggcgtttca tgtggcgaga tctgcgcttg tcctgtcgcg tccggcctcg
    10201 ttacgctctg gaccggcgct gcgggaagca gataatcccg tcctgcaggg ccagctgatg
    10261 ccgccgatat gcaggttcaa accatgttgc cacgctatgc actcctgctc gtcttctcat
    10321 cgctaggctc catcgccctg tctgctccgg gtccggcgga cctgcagatc ggccttcacc
    10381 tggcggactg cacggcaggc tcggcacacc ttcgggcgcg ttgtggcacc tttgtcgtgt
    10441 acgaggaccg cgcggccgcc tcgggccgca aggtcgagat cccgctcatc gtgatccccg
    10501 caaagcagcc gacccacaag ggcatcttct ggaatccggg cggcccagga gccggggcag
    10561 tcggtttcgc cggccagatc gccgatggcg aggtggcaaa ggagctgatg gcgctgcacg
    10621 agcactacga tatcgtgctc gccaacaacc gcggggtagg gggctcgagc tcgcagcagt
    10681 gtgatctcgc tccgcgtgat catcctgaat attatttcct gcagatctgg ccggatgccc
    10741 tgctcaaaca gtgccgggcg cgcctcggtg caaatgccaa cctcagtctg tacagcacca
    10801 tggtcagcgc cgatgacatg gaggacatgc gggccgcact caggtacgac cggatcgtgc
    10861 tcgacggggg ctcttacggc acctacttct ttctcgcgta tctgcgtcag caccccgagc
    10921 acgtggagag cgccattctc gatggggtag cccctccagg gctgctgacc attccgctgg
    10981 aggacgctgc cggagcgcag cttgcgatgg atcacctcat cgcggcctgc gcggcggatg
    11041 cgacctgcca cacgagcttt ccgcagctcg cccagcattt tgccgagctc gtccaccgtt
    11101 tcgccggcgg cccggttcgc atcaccatcg agaatccggc caccaagcgc ttgcaggcgg
    11161 tattcctctc caaggaggtt tttgcggacc gcctgcggca gacgctctac agcgcggggt
    11221 ccgcggcgta tgttccctac atcctcgagc gagcttacct cggcgattac gttccgctgg
    11281 ggcgaatgat cgacaccacg acccatcagc tgggctcgtt ggtggaagtg ggtttgaatc
    11341 tgtccgtgac gtgcgccgag gatattccct tcatcagcga agcggaggtt cgaagcagca
    11401 gtgccggttc attcgaaggt gacgttcgcg tcagggctca gcagcgcgcg tgcaagatat
    11461 ggaatgtcag cccggtcccc gccagcttca acgccccggt ccgcagcagc gtgcccgttc
    11521 tgatggtctc gggcagcagc gatcccgcca gtccagcgga gtatgggacg cgcgagctgg
    11581 cctatctgtc gaacgcacgg caggccctgg tgcggggcgc aggtcatacg gaagaaaccc
    11641 cctgcaccga tcgcttgaag gtcatgctcg ttctgaaagc cagcgttagc gagctggaca
    11701 ctgcatcctg caccagcgca ttcgagcgcc caccttttgc cacttccatg gcggggttcg
    11761 gctagggtcg gttcttactg cacagcttgc attcgccggg cccagccctt agttcgagag
    11821 ttccaggatt caaccggagc gtgacttctg atgatccgcc tgtttcgact ctggcgactc
    11881 ggccggcagg acctgcgcct cctctggtac gccctgcatc atccgagccg gcctgcgtgg
    11941 ctgtggccgg cagccctcgt gatggtggcg tatgcgctcg atccgctcaa tctcgccatc
    12001 ccggtcctgg gcgtggtcga cgatttcgtt ctgttgccgc tggcgctgca ctggctgctg
    12061 aagtggctgc cagccgatat ccgtctcgga tttgcccgac cctcgctcgt gcgctgagcg
    12121 agccgtccaa ggctcaatcg ccgctgagga ggccggctgt cacattgatg gcaaagccaa
    12181 gaatcgcgag attgaagaga aacgagatca ggctgtgcac aaccacagtc ctgcgaatat
    12241 tccgatcagc cgtggcgacg tcggcggtct ggcacgcggc cgcgatggtg aatgagaaat
    12301 aaacgaagtc ccagaacacc ggctgcttcg tttctggaaa cctcaggggg cggttcgctt
    12361 catctgcgct gtagaacaga tctgcgtaat gcagcgtaaa catggtgggc acgagcatcc
    12421 agcagtcgag gatcgtgagc gttgcgagca ggatgtgagc cactcgcgca gccggttcca
    12481 tgtgcttgac ggtcgagagc agcgccacga tcgccatcac gctgacgacc gccgcggcaa
    12541 ccaccgccac cagaataacg ggcgccgtcg gatcctcttc ctcgtagtac gctcgcaatt
    12601 gctgcgcatt cacgcgcagc aacttgatcc aggtcgccag cagcatgaac aatactgtgg
    12661 cgttccaagc ggtcagcgcc cgctcaattg ccgaccaggc tcgcggaagc aggcagaaca
    12721 gaagcaagcc cagcacgatg cccagccaga cccgtgtgtg attccacagc agcttttgcg
    12781 ccgtcgtgcg ctcaggcttg gtggagcttc ttacaaccat ccgcctaaga tagcagacag
    12841 cactaacgcg ggcgtcgcgg cgccgacggg actcacgctg gtaaacggcc cctcagaatc
    12901 tctccatcgt aggtgatcca ggcaagccgg agccgcagtc tgcccagggc agccgaccct
    12961 ccgtcccgta gtgatactgt ggcacaaagt cctcgggatg ctcgagggtg ccgatcaaca
    13021 cacccatctc ttccgcgccg tcatacttga ggtaaatcgg agtcccgcag ctggcacaaa
    13081 atccacgctg cgcgatcgga gacgagcggc gcgaggcggg aagacgggtc gcccactcga
    13141 tgctgctcgc cggaagccag acgagcacgg caaaagggcc gcccgtcgcg cgccggcaca
    13201 tgccgcagtg gcagtagtga actcggctgt gcttggccga cgctgaatag cggatcgcgc
    13261 cacagaaaca agtgcctgtt atcggcatcc cgtgccccgc ccaagtgacg atctgtctgg
    13321 ggattgagca gaaaacgtgc cgaagcttgc gtcggctggg aagaccaaag cgccttgaga
    13381 gagacggctt cagcggcgca gttgaagcgc gcgagcagct gccgtcatcg aagccgaccg
    13441 gcgcagtcag agccgctcga gctcctttgg aaactgcacc cagggatggc ggtatacctc
    13501 gtagacggag cgagccgggg gcggaaagcc tgggtcaccg aaagcaccga ccgcaaccgc
    13561 gatgagctcc ggtgccacgt cctgcgtgta ccacaccgtc gaaccgcaat gtgggcaaaa
    13621 gcgaaagtcc agcgcggtgc cgtcatctcc gattcggcgc cagatgcggg tttcgccttc
    13681 cgttgtgact ttgtctctag gccagcgcgc ctgcacgccg aaagcgctgc ccgtacgtcg
    13741 ctggcaagcg tgacagtgac agactcccac gcgggcaggc tctccctgca cgacgacgcg
    13801 aagctgcccg caggagcagg tggcggtacg tgtgttggtt tcggccattc ggatctacgg
    13861 gatgccgccg cagcaaggtt gtgccgcttg cacgggcgga caggggatgt tgccgtagga
    13921 gcagaacacg cagcaatccc ccggctgagg tcgcagcgtc gttccgcatc caacgcacgg
    13981 gtagaaatag atgcaagcat cggtgggcat cgttgcgagc tgccgatgcc cgcagattgg
    14041 gcagctcagc tcgctttgca gaatgagatc agccgccatc tccagcgtgc cctcagctca
    14101 attccccggg gcgactcggc gtgcccttct ttgcaggtct catcgggttg atgaccttac
    14161 ccgatctgag ggcgagatcc acggctgatg tcgtaagtca ttgaatattc gggtttcccg
    14221 aaggacattc gacctcgagt gactgagagg ctccgcaaaa aggattctcg gggcttgact
    14281 gcaccagtca attccataat tctagagata tggaatcaaa aagcgccgtg gatgctttga
    14341 gtgctctcgc gcaggagacg cgtctgcaga tctatcgttt gctcgtgcag gccgggccgg
    14401 acggccttcc tgcaggcgat gtggccgcgc gcgtcggcat tccggccaac actctgtcgt
    14461 ttcacctcaa agcgctgagt cacgctggtc tggtcacatc gcgccaggcg ggcagatttg
    14521 tttactacgc ggcgaactac gagcagatga cgacgctgct cgggtttctc acggaaaact
    14581 gctgtggtgg gcggagctgc ttgcctgctc ccgcacctcg aaggagaaag gtgtcatgaa
    14641 gcgcttccat gtccacgtgt cggtctcgga cctgaccgag agcgtcagct tctactcgca
    14701 actcttcggt gtcgagccga cggtgcacaa agctgattac gccaagtgga tgctcgagga
    14761 tccacgagtg aatttcgcga tctcatctca gcgccagccc gtcgggctca atcacctggg
    14821 ctttcaggtc gagagtgatg aggaattgca ccgcatgcgc actcagctgg cagccgctga
    14881 tgcccggatg atccaggagc aggcacagcc ctgctgctat tcccgcgccg acaagtactg
    14941 ggtgaccgac ccgacgggga ttgcgtggga gacattccat accctcggta gcatcccggt
    15001 gtatggcgaa gacacgtcgg tcttcgagca cggcgcctcg agtgttccgg caccttcggg
    15061 cacggcgaag gaatcgtgct gcattccggc agaaagaagt gctctcgagc cctgctgctc
    15121 gaaatgagtg gacgtcggcg cctgcgcagg tcgtctcggc tcaggtgttg acccaatcga
    15181 gcaagtcccg ggccaagcgt tcttgatggg tgaccaaaag gccgtgtgag gctccgtcat
    15241 agaccttgag ctggctgtta ccgatcattg tcacaagtcg cctgctggtg agctcgagc
//
LOCUS       DASRHN010000100         8396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2177287, whole genome shotgun sequence.
ACCESSION   DASRHN010000100 DASRHN010000000
VERSION     DASRHN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177287"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(643..1830)
                     /locus_tag="VFB37_03870"
     CDS             complement(643..1830)
                     /locus_tag="VFB37_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21618.1"
                     /translation="MTETHDLVGGKVHVYRRGTRGTWQCSTFLKGRNWRVTTKTDSLS
                     LAKEFAEDWYLELRGKSHAGLLEEKKKGKRFKEAAAKYLEELPILTHGQRSERYVNDQ
                     KKKLKVILPFLGDRYLSEAPGSISEYRIHRAQNCKTGKPPSRSTMHQEIVVIRQVYKS
                     AIRHGWFQFLPDFSVPYKASGKVVHRAWFSPEEYRTLYQATRKRAQNPKKPRWKRASE
                     NLHDYVLFMANTGLRPDEGARLRYRDVTIVHDEDSQERILEIEVRGKRGVGFCKSMPG
                     AVIPFERLRERNRPPSPDTKIFGKFQRELFNTILDELGLRIDRDGQHRTAYSLRHTYI
                     CMRLMEGADIYQVAKNCRTSVEMIEKYYAAHIKNMLDASAINVRKVRRKIKQVEVEEA
                     AEA"
     gene            2095..2679
                     /locus_tag="VFB37_03875"
     CDS             2095..2679
                     /locus_tag="VFB37_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO21619.1"
                     /translation="MATLAQIEVPPEVLAGARTGDEGAQAAIYAAVAPAAFGLIRRLA
                     GNRALAEDLFQETMLILYQRLGDFRGEAPLGAWVRQIAVTRCLMYLRSPWHRARLYAS
                     HAEDSDSWCESHLPVTPAPQAESLDLERALASLTPTARAVVWLFEVEGYSHQEIGQAF
                     GRSLSFSKSQLARAHGKLREWLEPRVDRQTCTPM"
     gene            2664..3302
                     /locus_tag="VFB37_03880"
     CDS             2664..3302
                     /locus_tag="VFB37_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21620.1"
                     /translation="MHTDVNHPSPLTDRLRQLPAELAPPYSWQEFKRRARPAASPARA
                     AMSWRHAAVAAVTVVGIVAVALWSRLNEPAPRTASTPVSIGDPHALGRAVGGDSLDRA
                     SRPGVSAPDGSAGEDSPSSGQRRAGGARPEALERWLAALPQEPAVVRVGTRAAVVGLE
                     DQLAQVDDLLNGERLDGGGQQRLAALQEQRTRLMGSLAQVRYAEALDAQASP"
     gene            3283..4458
                     /locus_tag="VFB37_03885"
     CDS             3283..4458
                     /locus_tag="VFB37_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZO21621.1"
                     /translation="MPRPHRERHFLRIGIEVFTVKYNSIILAMLLLLAGASASSLAAQ
                     PGAASEAVEEAGAAADKAGAQAEKAAAQADKATAQVDKATAQADQADADAESERSDAN
                     MKEMEAQLEAARARLEQAAHEVAALSARMSMPLVRNFLALGGDEFSRAIIGVELDPAS
                     GPGGARIEDVSPGGPAADAGLRRGDVIVSVNGTEVKGHDPARQVAHLMRNLAPDSKVK
                     VGVLREGKSREFVVTARRGLVLRDFPGVPAPPGVPAAPGAPAAPGAPALPAFPDGTFH
                     LPEVMMFRRPLAEMELATLTPQLGRYFGSDKGVLVVRAPKESGFKLEDGDVILAIDGR
                     EPTSGSHATRILGSYQPGEKISLRLMRDHKVLTVETTLPERERPQRKVRVLHDEAPA"
     gene            4486..5544
                     /gene="queA"
                     /locus_tag="VFB37_03890"
     CDS             4486..5544
                     /gene="queA"
                     /locus_tag="VFB37_03890"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZO21622.1"
                     /translation="MQRHDFAYELPPELIAQGPLPERSASRMLVLERSAASVEVPRRG
                     PPVMLDSTIRELPGLLRSGDLLVLNDTRVIAARLLGTKPSGGRVEIFLERALGGDEAL
                     AQMRASKPIRAGLQVSTAGGIVHILEREGDLWRIQLPGPALEFFEQWGEVPLPPYIDR
                     GATGADRERYQSVFAREPGAVAAPTASLHFDAPLLAEIQARGVRHAFITLHIGAGTFQ
                     PIRTQDVDSHTMHSERVTVRAETMETIEQTRAAGGRIVAVGTTVVRALESAALASSGR
                     GWSGETRLFIRPGFHFRIVDALLTNFHLPESTLLMLVCAFAGTEAVFAAYRHAVQARY
                     RFFSYGDCMFLTPSSEGR"
     gene            5541..6668
                     /gene="tgt"
                     /locus_tag="VFB37_03895"
     CDS             5541..6668
                     /gene="tgt"
                     /locus_tag="VFB37_03895"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZO21623.1"
                     /translation="MTPQFELLASEGAARRGRLTLAHGTVETPAFMPVGTYGTVKAMT
                     PEELEELGAQIVLGNTFHLMLRPGAAIIAAHGGLHDFMHWRHPILTDSGGFQVFSLKS
                     LRRISEEGVQFRSPIDGSEVRLTPEDSMAVQRHLRSDIAMALDDCTPYPATEEQARES
                     MERSMRWALRSHDCYYRQEAPGGLFGIVQGGVYPQLRLASLEALGARDWAGFAIGGLA
                     VGEPEEERLRILESLTPSLPADRPRYLMGIGRPEDIVAAVLRGVDMFDCVMPTRHARN
                     GHLFTSTGVINIRNSAHQSDLGPIDPGCTCYTCRHYTRAYLRHLDRCNEILGARLNTL
                     HNLHVYLQLMRSLREAIAAGGLTAWAHRYLEERRAAPDAVA"
     gene            6735..7067
                     /gene="yajC"
                     /locus_tag="VFB37_03900"
     CDS             6735..7067
                     /gene="yajC"
                     /locus_tag="VFB37_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZO21624.1"
                     /translation="MNALIPDAWAQAAPGAPGGSPFAMPILLGLFIVVFYFMLIRPQQ
                     KKAKEHQSLVSKLASGDEVVTNGGLLGRVTEVGDHFVTLEIADGVRVKIQKFQIASLM
                     PKGTLKSA"
     gene            7071..>8396
                     /locus_tag="VFB37_03905"
     CDS             7071..>8396
                     /locus_tag="VFB37_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21625.1"
                     /translation="MLEYARWKYILVVVVLLLGLLFALPNVFGDDPALQLARKDRAPV
                     AAAAQPEVESFLRGRNIAFERSYIDSGRLMLRFANVPEQLQARDAINDHYKDTYATAL
                     SFASRAPAFLRKMGLRPMPLGLDLRGGLYLLYQVDVNAAVSQLLDGYAQDARRVLSTA
                     NIPYKDVNVVTVGSDRPNGVRIVLPPEADVSAARSALAQTLQGLQVTTESLPSGSALV
                     ATLTQAQLRERQDYAMQQNITTLRNRVNELGVSEPIVQRQGLDRINVQLPGVQNSAEV
                     KDILGKVATLEFRLEDMQNSAFEAQQTGHAPLGSKLYTHTRIGRPILLKREVIATGDE
                     LTNATTTMQQEGPAVAIKLDARAGEAMLKTTRANINKRMAVVLIEKRRETSEVNGKKV
                     TKDVTDEQVINDATIRGIFSNNFVITGLSAGEARELALLLRSGSLATPVY"
BASE COUNT         1408 a         2670 c         2747 g         1571 t
ORIGIN      
        1 tgaggtcgag aaggatgctg tccgggccgc taggaggctt catttgcgaa tcacccgctt
       61 cgagctttga tcaaccgacg gcacggcgct tggatgcagc tggatccgcg gatcgaggga
      121 cgctccactt tcaggaatga caccgttcag tgacagtcag tacgctaggc agcgagccac
      181 ggcgcgaaag gagagcggcg ttcgccgcga aagagaattc ccctcggaaa ttttgaaagg
      241 aggagcgcca gcggcgctcg ggatgttatt tgtttgaaga gatggtgccc gaggccggaa
      301 tcgaaccggc acgcttttaa gggcggtgga ttttcttcct acttcggctt tcgccgccgc
      361 cgctcattgc gacgttcgta gtctggagca cgccttcacc atagtcttgc gaccgtaggt
      421 gcccgccgtc tgctctctac accttccccg gacttctggg gcttggctcg gcattagctc
      481 ggacttcgcg tccagggcct ctaccgagtt tgacgggcgt cacctccggg gtttcccccg
      541 gagggctcaa atttggcctg agtccactgc gtctaccaat tccgccactc gggcaacgca
      601 gccattgtat agcgccgcgc cgggtggtgt gccagcaaaa ggctatgctt cggcagcttc
      661 ttcgacctcg acctgcttga tttttcggcg gaccttgcgg acgtttatag ccgaggcgtc
      721 gagcatgttc ttgatgtggg cggcgtagta cttctcgatc atttcgacgc ttgtgcggca
      781 attcttcgcc acctggtaga tatcggcgcc ctccatgagg cgcatgcaga tgtaggtgtg
      841 ccggaggcta taggcggtgc ggtgctgccc gtcgcggtcg atcctaaggc cgagttcgtc
      901 gagaatggta ttgaagagct cgcgctgaaa tttcccaaaa atcttcgtat caggcgaggg
      961 cggtctgttg cgctctctga gtcgctcgaa gggtatgacg gcgcccggca tgctcttgca
     1021 gaagccaacg ccgcgtttgc ctcgcacttc tatttcgagg atccgctcct gcgaatcctc
     1081 gtcgtgcacg atcgtcacgt cacgataccg caggcgcgca ccttcgtctg ggcgcagtcc
     1141 cgtattggcc atgaacagga cgtagtcatg aaggttctcg gaggctctct tccagcgagg
     1201 cttcttgggg ttttgtgcgc gcttgcgggt ggcctggtac agcgtccggt attcctcggg
     1261 cgaaaaccac gcccgatgga cgactttccc cgacgcctta tacggcacgg aaaagtcggg
     1321 caggaattga aaccaaccat gtcggatggc gctcttgtag acctgccgga tgacgacgat
     1381 ttcctggtgc atggtactgc gggacggcgg cttgcctgtc ttgcagtttt gggcgcgatg
     1441 aatgcggtat tcggagatcg agcccggagc ttcagacagg tagcgatcgc ctaggaatgg
     1501 caggattact ttcagcttct tcttctggtc gttcacgtag cgctcgctac gttggccgtg
     1561 tgtgaggatg ggcaattcct cgaggtactt cgctgccgcc tccttaaacc ttttgccctt
     1621 cttcttttct tccagcaggc cggcgtggct cttgccgcgc aactcgagat accaatcctc
     1681 ggcgaattct ttggctagcg aaaggctgtc agtctttgtt gtaacccgcc agttgcggcc
     1741 tttgaggaat gttgagcact gccacgtgcc cctggttcca cgtcggtaga cgtgaacttt
     1801 cccaccaacg agatcgtgtg tttcggtcat ggctcgattc cttccgagac ctcaaaaggg
     1861 cgtaaacgtg taacggttgt gtaagggtat caggctcggt tgctttgcgc gatcttattg
     1921 tttttactgg ctaaaaaaga gattctggtt gctgaaggcg gattttgagt ccgctgcgtc
     1981 taccaattcc gccactcggg ccgcgagtgc cggccctggg cgcctcgccc gggcaggggc
     2041 gcgaattata gaccgcgttg cgacctttct ggccgccggc gcatctaacc caccgtggcg
     2101 acgcttgcgc aaatcgaagt cccgccggag gtgctggccg gggcgcgtac cggggacgag
     2161 ggtgcacagg cggcgatcta tgccgccgtg gcgccggctg ccttcgggct gatccgccgg
     2221 ctggccggca atcgggcgct ggccgaggac ctgttccagg aaaccatgct gatcctctat
     2281 cagcggctgg gtgattttcg gggggaggcg cccctcggcg cctgggtcag gcagattgcc
     2341 gtcacccgct gtctgatgta cctgcgctcc ccctggcatc gcgcccgcct gtacgcgagc
     2401 cacgccgagg actccgatag ctggtgcgaa tcccacttgc ccgtgacacc ggcacctcag
     2461 gcggagtccc tggatctgga gcgggcgctt gcgagtctca ccccgacggc ccgtgccgtg
     2521 gtgtggctgt tcgaagtcga aggctactcg catcaggaga tcgggcaggc cttcggtcgc
     2581 tcgctcagct tctcgaagtc acagcttgcg cgggcgcacg gcaaattgcg ggagtggctg
     2641 gaaccccgcg tggatcgtca gacatgcaca ccgatgtaaa tcatccctca ccgctcaccg
     2701 accggctgcg gcagttgcct gctgagctgg cccctccgta ctcgtggcag gagttcaagc
     2761 gtcgtgctcg ccctgcggca tcgcccgcgc gcgccgcgat gagctggcgc catgccgccg
     2821 tggccgcggt gaccgtggtt gggatcgtcg ctgttgctct gtggagccgt ctgaacgagc
     2881 ccgcgccgcg cacggcaagt acccctgtca gcatcggtga tccgcacgca ttgggtagag
     2941 cggttggggg cgactcgctc gacagggcct cgcgacccgg ggtcagcgcg cctgacggca
     3001 gcgccggaga ggattcgccg agctcggggc agcggagggc tggaggcgcg cgcccagagg
     3061 cgctcgagcg ttggctggca gccctgccgc aagagcccgc agtcgtgcgg gtgggcacgc
     3121 gtgcggcagt ggtgggcctg gaggaccagc tggcgcaggt ggacgatctg ctcaacgggg
     3181 agcgcctgga tggcggcgga caacagaggc tcgccgcgct tcaggagcag cgtacccgct
     3241 tgatgggctc gctcgcgcag gtgcgctatg cggaagcgct cgatgcccag gcctcaccgt
     3301 gagcgccact ttctcagaat cggaatcgag gtgttcaccg tgaaatacaa ctccatcatt
     3361 ctcgcgatgc tgcttctgct cgccggggct tccgcttcga gcctggctgc gcaacctggc
     3421 gcagccagtg aggccgtcga agaagcgggc gccgccgcgg acaaggccgg tgcccaggcc
     3481 gagaaagcag ccgcccaggc agacaaggct acagcacagg tggacaaggc gaccgcccag
     3541 gccgaccagg cggatgccga tgccgagagc gaacgcagcg atgccaacat gaaggagatg
     3601 gaggcgcagc tcgaggccgc acgcgcaagg ctcgagcagg ccgcccacga agtcgccgcc
     3661 ctgtctgccc gcatgagcat gccgctcgtg cggaacttcc ttgccctggg gggtgatgag
     3721 ttctcgcgag ccatcatcgg cgtggagctg gatccggcca gtggccccgg tggcgctcgc
     3781 atcgaggatg tcagtccggg tggccccgcg gccgatgccg ggctgcgccg cggcgatgtg
     3841 atcgtctccg tcaatggcac cgaagtgaag ggacacgatc cggcccggca ggtcgcccac
     3901 ctgatgcgta acctcgctcc cgacagcaag gtgaaggtgg gtgtactgcg ggagggcaag
     3961 tcacgggagt tcgtcgtcac cgcacggcga ggtctcgtcc tgcgagactt tccgggcgtc
     4021 cccgccccac ccggagtccc tgctgccccg ggggctccgg cagccccggg tgccccggcc
     4081 cttccggctt tcccggatgg cacttttcac ctgcccgagg tgatgatgtt tcgcaggccg
     4141 ttggccgaga tggagctggc cacgctcaca ccgcaactcg gccgctattt cggcagcgac
     4201 aagggtgtgc tcgtggtgcg cgcgccgaaa gaatccggct tcaagcttga ggatggcgat
     4261 gtgatcctgg cgatcgatgg gcgcgagccc accagcggct cgcacgccac tcggatcctc
     4321 ggctcctatc agccagggga aaagatctcc ctgcgtctca tgcgcgatca caaggtgctc
     4381 actgtcgaga cgaccttgcc cgagcgcgag cgtccgcagc gcaaggtccg ggtcctgcac
     4441 gatgaggcgc ctgcctgaga tgcgggtgat tgctaccatg aagcggtgca gcgtcatgat
     4501 tttgcctacg agcttccgcc ggagctgatt gcccaggggc ccctgcctga aagatcggcg
     4561 agccggatgc tcgtgctcga gcgctcggca gcgagtgtcg aggtcccgcg gcggggtcca
     4621 ccggtgatgc tcgacagtac catccgcgag ctgccgggcc tgctgcgttc cggcgatctg
     4681 ctcgtgctca acgacactcg cgtgattgcc gcgcgcctgc tgggcacgaa gccatctggg
     4741 ggccgtgtgg agatcttcct cgagcgggcg ctggggggag acgaggcctt ggcgcagatg
     4801 cgcgccagca agcccatccg cgccgggctg caagtgagca ccgccggcgg catcgtgcac
     4861 atcctggagc gtgagggcga cctgtggcgt atccagttgc ccgggcctgc tctggagttc
     4921 ttcgagcaat ggggagaggt gccgcttcct ccctatatcg atcggggtgc aacgggcgct
     4981 gaccgcgagc gctaccagag tgtgttcgca cgcgaaccgg gcgccgtggc cgctccgacc
     5041 gccagcctac acttcgatgc gccgctgctc gcggaaatcc aggcacgcgg cgtccgacac
     5101 gccttcatca cactgcatat cggcgccggc acgtttcagc ccatccgtac gcaggacgtc
     5161 gattcgcata ccatgcactc cgagcgcgtg accgtccgcg ccgagacgat ggaaacgatc
     5221 gagcagaccc gtgcggcggg gggccggatc gtggctgtcg gaacgaccgt ggtgcgcgca
     5281 ctggaatccg cggctctggc gtccagcggc cgcggctgga gcggcgaaac gcgcctgttc
     5341 atccgcccgg gatttcactt ccggatcgtc gatgcgctgc tgaccaactt ccatctgcct
     5401 gagtcaacat tgctcatgct ggtgtgtgcc tttgcgggca ccgaggcggt tttcgctgct
     5461 taccggcatg ccgtgcaggc tcgatacagg ttcttcagct acggagactg catgtttctg
     5521 actccttcct cggagggtcg ttgacaccgc agttcgagct gctcgcgagc gagggtgccg
     5581 cgcggcgcgg ccgcctcact ttggcccatg gcaccgtcga gaccccggcg ttcatgccgg
     5641 tgggcaccta tgggaccgtc aaagcgatga cccccgagga gctcgaggag ctcggagcgc
     5701 agatcgtgct gggcaatacg tttcatctga tgctgcgccc cggcgccgcc atcatcgcag
     5761 cgcacggcgg gttgcacgat ttcatgcact ggcggcatcc gatcctgacc gattcgggcg
     5821 ggtttcaggt cttcagcctc aagagcctgc gcaggatctc cgaggaaggc gtgcagttcc
     5881 gctcgcccat cgacggcagc gaggtccgtc tgacgccgga ggactcgatg gccgtgcagc
     5941 ggcatctgcg ctcggacatc gccatggcac tcgatgactg cacgccatat cccgccactg
     6001 aagagcaggc gcgtgagtcc atggagcgct cgatgcgctg ggcgctgcgc agtcatgact
     6061 gctactaccg ccaggaagcc cccggcggtc tgttcgggat cgtgcagggt ggggtctatc
     6121 cgcagctgcg tctcgcctca ctcgaggccc tgggcgcccg ggactgggcg ggcttcgcca
     6181 tcggcggcct ggcggtgggg gagccggagg aagagcggct gcgcatcctg gagagtctga
     6241 ctccaagctt accggcggat cggccccgct acctgatggg aatcgggcgc ccggaggata
     6301 tcgttgccgc ggtactgcgc ggggttgata tgttcgattg cgtgatgccg acgcgccatg
     6361 cacgcaacgg ccatctattt acttctaccg gtgtcatcaa catccgcaac agcgcgcacc
     6421 agagcgacct cggcccgata gatccgggct gcacctgcta cacctgccgg cactacacgc
     6481 gtgcctacct gcggcacctg gaccgctgca atgagatcct cggagcgcga ctgaacaccc
     6541 tccacaacct gcacgtctac ctgcaactca tgcgctcact gcgcgaggcg atcgccgctg
     6601 ggggcctcac cgcctgggcc catcgttatc tggaggagcg gcgggccgcg ccagatgctg
     6661 tggcataatg ccgccccttc gctccggccg gccgccatgc cccgcccggg gcacctccga
     6721 gccaagggtg acgcatgaat gctttgattc ccgatgcctg ggcccaggcg gcgcccgggg
     6781 cccccggcgg cagcccattt gccatgccca tcctgctggg cctgttcatc gtcgtcttct
     6841 atttcatgct gatccggccc cagcagaaga aggcgaagga gcatcagagc ctcgtctcca
     6901 agctcgcgag cggcgatgag gtcgtgacga acggcggcct gctcgggcgt gtcacggagg
     6961 ttggggacca cttcgtgaca ctggagatcg ccgatggagt gcgggtgaaa atccagaagt
     7021 tccagatcgc ttccctgatg cccaaaggca ccttgaaaag cgcctgaacc atgctcgaat
     7081 acgcccgctg gaaatacatc ctggtagtgg tcgtgctgct gctgggcctg ctgtttgccc
     7141 tgcccaacgt gtttggggat gacccggctc tgcagctggc ccgcaaggac cgggcgccgg
     7201 tggccgccgc tgcgcagccg gaggtcgaat cgtttctgcg cgggcgcaac atcgccttcg
     7261 agaggagcta catcgatagc ggccgcctga tgctgcgctt cgccaatgtg cccgagcagc
     7321 tgcaggcgcg cgatgccatc aacgatcact acaaggacac gtacgccacg gcgctgtcat
     7381 tcgcctcccg agcgccggcg ttcctgcgca aaatgggtct gcgaccgatg ccgctcggcc
     7441 tcgatctgcg cggaggcctg tacctgctct accaggtcga cgtcaacgcc gccgtcagcc
     7501 agcttctgga cggctacgcc caggacgcac ggcgcgtttt atcgaccgcc aacattccct
     7561 acaaggacgt caacgtggtg acggtcggca gcgaccggcc gaatggcgtg cggatcgtcc
     7621 tgccgcccga ggccgatgtc agtgccgccc gttccgccct cgcgcagacg ctgcaagggc
     7681 tgcaggtcac gaccgagagt ctgccgagcg gctccgcgct ggtggcgacc ctgacgcagg
     7741 cgcaactccg ggagcgtcag gactacgcga tgcagcagaa catcaccacg ctgcgcaatc
     7801 gcgtcaacga gctgggcgtg tccgagccga tcgtgcagcg tcaggggctc gaccgtatca
     7861 acgttcagct gcctggagtg cagaattcgg ccgaagtgaa ggacattctc ggcaaggtgg
     7921 caacgctcga gttccggctc gaggacatgc agaacagcgc cttcgaggcg cagcagaccg
     7981 gtcacgcgcc gctaggatcg aagctctaca cccacacgcg catcggccgc cccatcctgc
     8041 tcaagcgcga ggtcattgcc acgggcgatg agctcaccaa tgccactacc accatgcagc
     8101 aggagggtcc ggcggtcgcg atcaagctgg atgcgcgcgc gggagaggca atgctgaaga
     8161 ccacccgcgc caacatcaac aagcggatgg ccgtcgtgct catcgaaaag cgacgcgaga
     8221 cgagcgaggt caacggcaag aaggtcacaa aggatgtcac tgacgagcag gtgatcaacg
     8281 atgcgacgat tcgcggcatc ttcagcaata acttcgtgat caccggcctg tctgcgggtg
     8341 aggcacgcga gctggctctg ctgttgcgct ccggctcgct cgccacgccg gtgtat
//
LOCUS       DASRHN010000101         5603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_364560, whole genome shotgun sequence.
ACCESSION   DASRHN010000101 DASRHN010000000
VERSION     DASRHN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364560"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /gene="aspS"
                     /locus_tag="VFB37_03910"
     CDS             complement(<1..1229)
                     /gene="aspS"
                     /locus_tag="VFB37_03910"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZO21626.1"
                     /translation="MRSHYCGQVDEKLIGQAVTVAGWVHRRRDHGGVIFVDLRDREGL
                     LQIVFDPEAAEVFREAERLRNEFVVEVTGRVRERPGGTANANLASGRVELLANSIELL
                     NRSEPLPFQLDEQVGEEVRLRYRYLDLRREEMTQRLRLRHRITRAMRRYLDDHGFIDL
                     ETPMLTKATPEGARDYLVPSRTHPGKFFALPQSPQIFKQLLMISGFDRYYQIVRCFRD
                     EDLRADRQPDFTQLDIETSFLTQDAIMGLMEGLVRYFFKDVLSVALADPLPRMTFAEA
                     LRRYGSDKPDLRIALELVDVADLVRECDFKVFATPARDPRGRVAALRVPGGAAMPRSQ
                     IDEYTAYVGRYGAKGLAYIKVNDVSKGREGLQSPIVKFLSDAAVTGILERTRAEDNDL
                     IFFGADTYKIVSDALGAL"
     gene            complement(1243..1911)
                     /locus_tag="VFB37_03915"
     CDS             complement(1243..1911)
                     /locus_tag="VFB37_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZO21627.1"
                     /translation="MAGPAKRRGGLRRYLVAGILIWLPILATIWVVTFIIGLMDRTLL
                     LLPSAYRPQALVGFALPGLGALFAFAVVLATGLLVTNLIGRQLVLYWEELLQRIPVVR
                     TVYSGVKSFAESVFSQSNSFRKVVMVEYPRLGMWSLGFMTAEDVPEVSVRTGEPHACV
                     YISNALNATAGFLVIVPRRQIVELEMSVDAAMKMIITCGVVVPQTALAKPPIEPVAGV
                     AGAP"
     gene            complement(1968..2459)
                     /locus_tag="VFB37_03920"
     CDS             complement(1968..2459)
                     /locus_tag="VFB37_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZO21628.1"
                     /translation="MPFYEYECSNCKFYTEIMQKISDAPLTKCPSCGRRKLKKLVSAP
                     VFRLKGAGWYETDFKSDKEKKRNLAGADKEEAKPEATKTEGGEAKADAKATKADSGSA
                     AASAAGESKPAKVAAESRGAGRARSLPAAKSAGRAGVSAKVRPSASRSKAKSGSGRKK
                     GRR"
     gene            complement(2506..5079)
                     /gene="clpB"
                     /locus_tag="VFB37_03925"
     CDS             complement(2506..5079)
                     /gene="clpB"
                     /locus_tag="VFB37_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZO21629.1"
                     /translation="MNPEKLTSRFQQALTEAQSLALGRDHQFLEPAHVLLAMLDSQGG
                     SVRPLLLKAGLDINRLRSELQAQLDRLPRVQGAPGDIHISNELNRVFNGADKLAQQRG
                     DQFISSELFVLACFEDRNVARALKDAGGVRGAIEKAIDEVRGGEKVSDPNAEESRQAL
                     EKYTIDLTARATSGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGDPGVGKTAIVEGL
                     AQRIINGEVPEGLKNKRILALDMGALIAGAKFRGEFEERLKGVLNDLAKQAGQVILFI
                     DELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQK
                     VLVNEPSVEDTIAILRGLQERYEVHHRVDITDPAIVAAATLSHRYIADRQLPDKAIDL
                     IDEAASRIRMEIDSKPEALDRLERRIIQLKVEREALKKEQDVASRKRLATLQESLSGY
                     EKEYADLEEVWKSEKAALQGAAQVRADLDRVRGELEAARRAGDLQRMAELQYGRIPEL
                     EKRLQQALDAENKAPQLVRNRVTEEEIAEVVSKWTGIPVSKMLEGEKEKLLRMEEALA
                     RRVVGQDEALRIVANAIRRTRAGLSDSRRPNGSFLFLGPTGVGKTELCKALAEFLFDT
                     EDALVRVDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEIEKAH
                     QDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGSQVIQEYAGEKNYVRMKAAV
                     MEIVQQSFRPEFINRIDDIVVFHPLGTEQIRAIVDIQLAYLRKRLQERNMDLTLEDSA
                     RDLLGEAGFDPVYGARPLKRAIQQQIENPLAQRILQGAFAPGDRIRVRARGEELVFEK
                     A"
     gene            complement(5152..>5603)
                     /locus_tag="VFB37_03930"
     CDS             complement(5152..>5603)
                     /locus_tag="VFB37_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZO21630.1"
                     /translation="GAAGVLVLVGDSIHNVLDGVLIAAAFLTDVPLGLMTTFAVAAHE
                     IPHRVGDFAILVHTGLSRRRALCLNMATGLGSVLGGVVAWFGLQRAIGIVPYALAFAA
                     AGFLYIAAAGLIPGLHRRTDPGTSLVQIVLMGLGAAVIAWAQSLAHR"
BASE COUNT          985 a         1914 c         1715 g          989 t
ORIGIN      
        1 agtgcaccga gcgcatcgct cacgattttg tacgtgtcgg caccgaagaa aatgagatcg
       61 ttgtcctccg cccgcgtgcg ctcgagaatc ccggtcacgg ccgcatcgct gaggaacttg
      121 acgatcgggg actgcagacc ctcgcgcccc ttcgaaacat cgttcacctt gatataggcg
      181 aggcccttgg cgccgtaacg tccgacgtag gcggtgtatt cgtcgatctg cgagcgcggc
      241 atcgctgcgc cgccgggcac tcgcagggca gccacgcgcc cgcgcggatc gcgcgcaggc
      301 gtcgcgaaca ctttgaaatc gcactcgcga accagatcag ccacatccac gagctcgagc
      361 gcgattcgca ggtccggctt gtccgaccca taccggcgca aagcctcggc aaaggtcatt
      421 cgcggcaacg gatcggcaag cgcgacactc aacacgtcct tgaagaagta gcgcacgagc
      481 ccctccatga gccccatgat cgcatcctgc gtgaggaacg aggtctcgat gtcgagctgc
      541 gtgaaatcgg gctgacggtc ggcacgcaaa tcctcgtcgc ggaagcagcg cacgatctgg
      601 taataccggt cgaacccgga aatcatcagc aactgtttga agatttgtgg cgactgcggc
      661 agcgcgaaga atttgcccgg gtgcgtgcgg ctcgggacga gataatcgcg cgccccctcg
      721 ggagtcgcct tggtcaacat cggcgtctcc agatcgatga acccgtggtc atcgagataa
      781 cgccgcatcg cacgcgtgat ccggtgacgc agacgcaggc gctgagtcat ctcttcgcgg
      841 cgcaaatcca gatagcggta acgcaggcgc acctcttcgc cgacctgctc atcgagctgg
      901 aacggcaacg gctcggagcg gttgagcagc tcgattgaat tcgccagcaa ctcgacgcga
      961 ccggaagcga gattggcgtt cgccgtcccg cccgggcgct cgcgcacgcg gccggtgact
     1021 tcgacgacga attcgttgcg caggcgctcg gcctcccgga acacctcggc ggcctccgga
     1081 tcgaagacga tctgcaggag gccttcgcga tcgcgcaggt cgacgaagat caccccgcca
     1141 tggtcccggc gccggtgcac ccagcccgcg accgtgacgg cctgtccgat cagcttctca
     1201 tcgacctgtc cgcagtaatg tgagcgcata aggcgcgcga tactacggag cccccgctac
     1261 ccccgcaacc ggctcgatcg ggggcttggc cagtgccgtc tgggggacca ccaccccaca
     1321 ggtgatgatc atcttcatcg ccgcatcgac gctcatctcg agctccacga tctgtcgccg
     1381 cggcacgatc acgaggaagc cggccgtggc attcagcgcg ttggagatgt agacgcaggc
     1441 gtgcggctca ccggtcctca cactgacctc tggcacatct tcggcggtca tgaatcccag
     1501 gctccacatg ccgagccggg ggtactccac catcacgacc ttgcggaacg aattcgactg
     1561 cgagaagacg ctctcggcga aactcttgac gccgctgtac accgtgcgca ccaccggaat
     1621 gcgctgcaac agctcctccc agtagagcac gagctgccgt ccgatcagat tcgtcaccag
     1681 cagtccggtg gccaggacga ccgcaaaggc aaacagggcc ccgaggccgg gcagcgcgaa
     1741 tcccaccagc gcctgcggcc gataggcaga aggcagcagc agcagagtgc gatccatgag
     1801 cccgatgatg aaagtcacga cccagatcgt ggccaggatc ggcagccaga tcaggatccc
     1861 ggcgacgaga tagcgccgca gaccgcctct ccttttggct ggtccggcca agagtcaccc
     1921 caccggggtg cagggcgcca agcgcgcgag ggctatctgc cgcctcttca cctgcgtccc
     1981 ttcttgcgcc ccgaaccgct tttcgccttg ctgcgcgaag cagacggacg cactttggcc
     2041 gagacgccgg cgcgcccggc cgatttagcc gcagggagag atcgtgccct gcccgcgcca
     2101 cgggactcgg cagccacttt cgcaggcttc gactcgccgg cggcgctcgc cgcggccgag
     2161 ccgctgtccg ccttggtggc cttcgcgtcg gcttttgcct cgccgccctc ggtcttggtg
     2221 gcctcgggct tcgcctcctc cttgtccgcc ccggccagat tgcgcttctt ttccttgtcc
     2281 gacttgaaat ccgtctcgta ccagccggcg cccttgaggc ggaataccgg tgccgaaacc
     2341 agcttcttca gcttgcgtcg gccgcacgaa gggcacttcg tcagcggagc atcgctgatc
     2401 ttctgcatga tctcggtgta gaacttgcag ttcgagcact cgtattcgta gaacggcatg
     2461 acactcgtgg gttttctggc gtgaagaagc ggcgattata gggtctcagg ccttctcgaa
     2521 caccaactcc tcgccccggg cgcgcacgcg gatccggtcg ccgggcgcaa aggcgccctg
     2581 cagaatccgc tgcgcgagcg gattctcgat ctgctgctga atggcccgct tcaggggccg
     2641 ggcaccataa accggatcga aaccggcctc gcccagtaga tcccgtgcgg agtcctcgag
     2701 cgtcaggtcc atgttgcgct cctgcaggcg cttgcgcagg taggcaagct gaatgtccac
     2761 gatcgcgcgg atctgctcgg tgccgagcgg gtggaacacc acgatgtcat cgatccggtt
     2821 gatgaattcg ggccggaagc tctgttgcac gatctccatc acggccgcct tcatgcgcac
     2881 gtagttcttc tcgcccgcat attcctggat gacctgcgag ccgagattcg aggtcatgat
     2941 gatgaccgtg ttgcggaagt ccacggtgcg gccctgaccg tcggtcagcc gtccatcatc
     3001 caacacctgc agcaggacat tgaagacgtc ctggtgggcc ttctcgatct catcgagcag
     3061 aatcaccgaa taggggcggc gccggaccgc ctcggtgaga tagccgccct cctcgtaccc
     3121 gacgtatccg ggaggggcgc cgatcaggcg cgagaccgag tgcttctcca tgaactcgga
     3181 catatccacc cgcaccagtg cgtcctcggt gtcgaacagg aactccgcca gagccttgca
     3241 cagctcggtc ttgccgacac cggtcgggcc gagaaacagg aaggatccgt tcgggcgccg
     3301 cgaatccgac aggccggcac gcgtgcgccg gatcgcgttg gcgacgatgc gcagcgcctc
     3361 atcctgtccc accacacgtc gcgcgagagc ctcctccatg cgcagcagct tttccttctc
     3421 gccctcgagc atcttcgaga ccgggatgcc ggtccacttg gagacgacct ccgcgatctc
     3481 ctcctcggtc acccggttgc gtaccagctg cggcgccttg ttctccgcat ccagcgcctg
     3541 ctgcagccgc ttctccagct ccggaatccg cccgtactgc aactcagcca tgcgttgcaa
     3601 atcgccggcg cggcgcgccg cctcgagctc gccgcgcacg cgatcgagat ccgcccgcac
     3661 ctgcgctgcg ccctgcagcg ccgccttctc ggatttccac acttcctcga ggtccgcgta
     3721 ctccttctcg tagccgctca gactctcctg cagtgtggcg aggcgcttgc gggaggcgac
     3781 atcctgctcc ttcttcagcg cttcccgctc caccttgagc tgaatgatgc ggcgctcgag
     3841 ccggtccagt gcctcgggct tcgagtcgat ctccatgcgg atgcgcgaag cggcttcgtc
     3901 gatcagatcg atcgccttgt cggggagctg ccgatcggcg atgtaacgat gggacaaggt
     3961 cgccgccgca acgatcgccg gatccgtgat gtcgacccga tggtgaacct cgtaccgctc
     4021 ctgcagtcca cgcaggatcg cgatggtgtc ctccacggag ggctcattca ccagcacctt
     4081 ctggaagcgg cgctcgagcg cggcatcctt ctcgatgtac ttgcgatatt catcgagcgt
     4141 cgtggcaccc acacaatgca gctcaccgcg cgccagggcc ggcttgagca tgttgccggc
     4201 atccatggcg ccttcggcct tgccggcccc aaccatggtg tgcagctcgt cgatgaacag
     4261 gatcacctgt cccgcctgct tggccagatc attgagcacg cccttgagac gctcctcgaa
     4321 ctcgccgcga aacttcgcac cggcgatcaa cgcgcccata tcgagcgcga gaatgcgctt
     4381 gttcttcaga ccctcgggga cttcgccgtt gatgatccgc tgcgccaggc cctcgacgat
     4441 cgccgtcttg ccgacacctg gatcgccgat cagcacgggg ttgttcttgg tgcgccgctg
     4501 tagcacctgg atggtccgcc ggatctcatc gtcgcggccg atgaccggat cgagcttgcc
     4561 ggaggtggcc cgcgccgtca ggtcgatggt gtatttctcg agcgcctggc ggctctcctc
     4621 ggcattggga tccgacacct tctctcctcc tcgcacctca tcgatcgcct tctcgatggc
     4681 accgcgcacg ccgccggcat ctttcagcgc acgagcgaca ttgcggtcct cgaagcaggc
     4741 gagcacgaac agctcgctgg agatgaactg atcgccgcgc tgctgagcca gcttgtcggc
     4801 accgttgaac acccggttga gctcgttgga gatatggata tcgcccggtg cgccctgcac
     4861 tcgcggcagg cgatcgagct gcgcctgcag ctccgagcgc agccggttga tatccagtcc
     4921 ggccttcagc agcagcggcc gcacgctgcc gccctgactg tccagcatgg ccagcagcac
     4981 atgcgcaggc tccaggaatt gatgatcccg gcccagcgcg agggactgcg cctcggtgag
     5041 cgcctgctgg aagcggctgg tgagtttttc ggggttcatg ggggagcctg acggaagttt
     5101 cggctgcgga cggtatgggg gcaatcccca ggtgcttcaa ggcgctacgc gctatcggtg
     5161 cgccagcgat tgcgcccagg cgatcacggc ggcgccgagg cccatgagca cgatctgcac
     5221 caggctggtc cccggatcgg tccgccggtg cagccccggg atcagcccgg cggcggcgat
     5281 gtagagaaag ccggcggcgg caaaagcgag cgcatagggc acgatgccga tcgcccgctg
     5341 caggccgaac caggccacca cgccccccag cacggagcct agaccggtgg ccatgttcag
     5401 acacagtgcc cgcctgcgcg acagaccggt gtgcacgagg atcgcgaaat cgccgacccg
     5461 gtgcggaatc tcatgtgcgg ccacggcaaa ggtagtcatc agtcccaggg gcacatcggt
     5521 caggaaggca gcggcaatca gaacgccatc gagcacattg tggatgctgt cgccgacgag
     5581 aacgagcacg cccgcggcgc cca
//
LOCUS       DASRHN010000102        10357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2012871, whole genome shotgun sequence.
ACCESSION   DASRHN010000102 DASRHN010000000
VERSION     DASRHN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012871"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VFB37_03935"
     CDS             complement(<1..1039)
                     /locus_tag="VFB37_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZO21631.1"
                     /translation="MNSMPSRLANIWAALRPDPEKTSLGWWMVAIHVAIVLLVGGGIS
                     YSATRILHALADEQGKARAQLAGAMAREDLRRLGEDTLTAARALAERPTLQRLVAEKR
                     AEAIPPLLRRSCEAAGIDACALFSGTQRIAQSGPDLDWDGILTASAEQGATFLALPAN
                     VRIPVFGAYAGLGDTDGTRVFAVRMLNEKLARVLSQQVGLEIRLIDYRSFTNDPVNAF
                     TPLYSASLADGRSAVQRIESQHVYASSVPVFASTGEAIALIEALLPSAEIDSSAARLT
                     NKLLMTALILAALAVFGGVILSQLVAGPVRALTQAAVRLGQGDFSTSIPPGGAAEVGT
                     LARTMEDMRRNL"
     gene            complement(1036..1905)
                     /gene="cysW"
                     /locus_tag="VFB37_03940"
     CDS             complement(1036..1905)
                     /gene="cysW"
                     /locus_tag="VFB37_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZO21632.1"
                     /translation="MAAGAHTLGLEDLDTRPGRDTLLASLVRWLFISVSLVFLAALLF
                     APLATVFVMALEHGWRAYVQTFADPDTTAAIRLTLLTALVVVPINTVFGLAASWAIAK
                     FEFKGKSFLITIIDLPLAVSPVISGMMYVLLFGLHGWFGVWLQEHDISIIFAVPGIML
                     ATMFVTFPYVARELIPLMQQVGNDEEEAAMTLGAGGWITFFRVTLPKVRWGLVYGVIL
                     CNARAMGEFGAVSVVSGHIRGLTNTMPLHIEILYNEYNFVGAFAVASLLTLLALMTLL
                     VKTLVERTGYRRR"
     gene            complement(1905..2744)
                     /gene="cysT"
                     /locus_tag="VFB37_03945"
     CDS             complement(1905..2744)
                     /gene="cysT"
                     /locus_tag="VFB37_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZO21633.1"
                     /translation="MSTFRQPSVLPGFSLTFGFTTLFLSTIVLIPLAALVLMTASMHW
                     SEFVRIVLSPRALGSYRLSFGASMLAAFINLVFGFIIAWTLVRYEFPGRKLIDALIDM
                     PFALPTAVSGIALTTVFAENGWIGSYLHPLGIKVAYTWIGVTVALTLIGLPFAVRSVQ
                     PALLEVQRDLEDAAETLGAGWLYVFRRIILPTVLPALLTGFTLAFARAVGEYGSVIFI
                     AGNLPMKTEITPLLIVIHLEEFDYKGAAALGFVMLVISFVVLLTINLLQAWGRRRIVA
                     VSR"
     gene            complement(2741..4528)
                     /locus_tag="VFB37_03950"
     CDS             complement(2741..4528)
                     /locus_tag="VFB37_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO21634.1"
                     /translation="MSITLDQVTKRYQGVPVVNDVSLDIGDGEFFVLLGPSGSGKSTL
                     LRAIAGLAGVDHGRISLHGRDVTHVAARERGVGLVFQNYALFRHMTVADNIEFALRVR
                     RMRAADRRRRRQELLRLVALEGMDERLPAQLSGGQQQRVAVARALAHKPEVLLLDEPF
                     GALDAKIREELRRTIRQVQRELGITTVLVTHDQEEAFALADRIGVMNLGRLLELGRPH
                     ELYTRPATRFVATFLGAANLILARQSKNGIRFGQTPVEASASLPSPFHADREHEVVAV
                     VRPEEIEIAPTRERLTSNYLTRGAVDELLFTGALERIRIRIDESADGAALSSDGSGSA
                     LLEATRTQHEQRAFRIRPGEQVVIGVRRVHILPTPLSSFTACASTEGLAQALLREPLL
                     SELALRMKTRISARVEPRLGMPGAGAEGRSNFAGTTVIASHADAVFHVQWLLERGAEE
                     ILILPQHASAPQRVLIHWADQSLRRATLAVSASILRHLSAEAVYVDILPEQTPMAQRP
                     HGMRALLDARSEAQALHGLEIRTELRFGEASSELARHLAESAGQMLILGIQETAGLAA
                     RFGSLLSSAAWPMLIVHRQHESALAEVTS"
     gene            complement(4683..4949)
                     /locus_tag="VFB37_03955"
     CDS             complement(4683..4949)
                     /locus_tag="VFB37_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HZO21635.1"
                     /translation="MVEPPPLLRFPTDYPLKIVGRPSEEFRKRVRAIMLRHVPGLDPT
                     RVSERLSENGNFLSISYVIVAESSDHVIGLIKDLKACEGVLTII"
     gene            complement(4943..5815)
                     /locus_tag="VFB37_03960"
     CDS             complement(4943..5815)
                     /locus_tag="VFB37_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HZO21636.1"
                     /translation="MAEPLPLAYLNGAFLPLAEARISPLDRGFLYGDGVYEVMPVYGG
                     RPFRFEAHAERLTRSLKQVRMDDPHTRQEWRELLGTLIARNGGGQQYVYWQVTRGAEK
                     GRNHAPLPLIPRTVFAFCAPLPPIAAATLESGLACVTAEDTRWAQCDIKSVALLANVL
                     LRQLAVDAGAGETILLREGELMEASSSAVQVVIDGVVRAPPNSRKILPGTTRSAVEEM
                     AERAGIAYTAAPVSEAELRNAAEVWLSSATREVQPVTRIDGKPVGDGKPGPLWRRVFD
                     ELQRYKRELADRPW"
     gene            complement(5992..7029)
                     /locus_tag="VFB37_03965"
     CDS             complement(5992..7029)
                     /locus_tag="VFB37_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HZO21637.1"
                     /translation="MRSIVSKLALVATLCACASLQAASGRGAPGSYFDSSGRDDVLSG
                     GVKIITIQTPKGPFHVWTKRVGNNPSIKVLTLHGGPGATHEYLEALDSYFPGAGIEYY
                     YYDQLGSAYSDQPDEPTLWDLPRFVEEVEQVRKALKLDRSNFYLYGQSWGGMLALEYA
                     LKYQQNLKGLIISNMVASIPAYNDYAHKVLMPAMDQKALAEILRLEAAGRYEDPRYEE
                     LLTQNFYVYHVLRMPPDQWPDPVLRAFRKTNRKLYVPMQGPSEMGASGKLEKWDRTAD
                     LPRITVPTLAIGARYDTMEPAQMERIARSVHKGRYLYCPNGSHLALYDDQKTYMSGLI
                     RFIQDVDSGRF"
     gene            complement(7064..9007)
                     /locus_tag="VFB37_03970"
     CDS             complement(7064..9007)
                     /locus_tag="VFB37_03970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZO21638.1"
                     /translation="MSPASPAPAAASSSAIGDASGGPIPNPYTTAHTLDKTDLEAWLD
                     GFMPYAIENGDIAGAVVTVVKDGELIFAKGYGYSDVAHHTPVDPERTLFRPGSVSKLF
                     TWTAVMQMVEQGKLDLDRNINDYLDFHIPDRPDGPITLRDVMTHTPGFEEQIKSLIAD
                     NPKAVVPLAQYARDFTPTRIFKAGSTPAYSNYATAVAGYLVQRVSGQSFDDYIDEHIF
                     KPLGMTHASFRQPLPATLLPEVSQGYRVASEPEKPYEYVVPAPAGSLAASGEDMAKFM
                     IAHLQDGEFHGQRILKAQTAEEMHTTALTMVPPLNRMDLGFYEQNYNGHRIISHGGDT
                     VWMHSYLHLFLDDHVGLFMSFNSQGRQGAAGQVRQALFDEFVDRYFPGPWPMGSVPKD
                     IAAQHAHMMVGYYDDSRRADRSFMSLIQILGPAKVATAKDGTLTMSLMMGRNGVLRHY
                     QEVAPFVWRDAASGWRMAAKVVDGRVVRVSMDELSPFMVFDPTPGWRSPAWLVPAIVL
                     ALCALVLTGALWPIAAISRRRHNFRLPVTGLAWRGHLISRLAAIALVVMSLAWLTLLI
                     SGTKSLAVLTASLDPLLLLMYLLSVVVYIGGTAAMLYSVWVAWRTARPLGALIWTSIL
                     ALCSLVLLYVAILYHLMSFVTRY"
     gene            9296..10324
                     /locus_tag="VFB37_03975"
     CDS             9296..10324
                     /locus_tag="VFB37_03975"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HZO21639.1"
                     /translation="MSRPLVQFRVPFVVFLGDLADETFCKTGMGLVQWRRQECVGQIR
                     LPGCGADAEVPDLDVSQAKAAGARSLIVGSAVVGGGLPEGWIDVLCEAARSGIDIVAG
                     LHVRLATLPGLREAAEAGGARLIDVRVPPSGLPVGTGQKRSGRRLLTMGTDCACGKKY
                     TALALDAELRRRGVESSFRATGQTGIMIAGEGIPLDAVVADFVTGAAELLSPAASPGH
                     WDVIEGQGSLFHPGYLQVTVGLLVGSQPDAFVVCHDPVRESISGWPDFPLPTPAQVIA
                     RTVELGRMTNPAIRCVGISLNLSKVRPEDRARVLEQYAVQTGLPCCDPLLGGVGPIVE
                     SLLRAFPA"
BASE COUNT         1920 a         3234 c         3416 g         1787 t
ORIGIN      
        1 ccaggttgcg ccgcatgtcc tccatcgtgc gcgccagggt gcccacttcg gccgcgccac
       61 ccggtgggat ggaggtcgag aaatcgccct gcccgagccg gactgcagcc tgggtcagtg
      121 ctctgaccgg tccggcgacc agctgactga gaatcactcc gccgaacacc gccagagcgg
      181 cgaggatcag tgccgtcatg agcagcttgt tggtcagtct cgcggcggag ctgtcgatct
      241 cggcactcgg cagcagggcc tcgatgagcg cgatggcttc gcccgtcgag gcaaataccg
      301 gcacgctgga ggcatagacg tgctgcgatt cgatgcgctg cacggccgag cgcccatccg
      361 ccaggcttgc ggagtagagc ggcgtgaatg cgttcacggg atcgttcgtg aacgagcgat
      421 agtcgatcag tcgaatctcg aggccgacct gctggctcag cacccgcgcg agcttctcat
      481 tcagcatccg taccgcgaag acacgcgtgc cgtccgtgtc gccgagcccg gcgtatgcgc
      541 caaacaccgg gatgcgtaca ttggcgggaa gcgccaggaa ggtcgcgccc tgctcggcgc
      601 tcgcagtgag gatgccgtcc cagtccagat ccggtccact ctgcgcgatg cgctgcgtgc
      661 ccgagaagag cgcacaggca tcgatgccgg cggcctcgca ggacctgcgc agcagaggtg
      721 gaatcgcctc ggcgcgcttt tcggccacca ggcgttgcaa tgtcgggcgc tcggcaagcg
      781 ctcgcgctgc ggtgagcgtg tcctcgccca agcggcgcag gtcctcgcga gccatggcgc
      841 ctgcgagctg tgcgcgtgcc ttgccctgct catccgccag tgcgtgcagg atccgcgtcg
      901 ccgagtagga gatgccacca cccacgagca gcacgatggc cacatggatg gccaccatcc
      961 accagccgag gctggttttc tcaggatcgg gcctcagcgc ggcccagatg tttgccaggc
     1021 ggctcggcat cgagttcatc gccggcggta accggtgcgc tcgaccagtg tcttgaccag
     1081 cagcgtcatg agcgcgagca gggtcaggag cgaggccacg gcgaatgcgc cgacaaagtt
     1141 gtactcgtta tagaggatct cgatgtgcag cggcatcgtg ttggtcaagc cgcggatatg
     1201 acccgaaacg accgagacgg cgccaaattc gcccatggcg cgcgcattgc acagtatcac
     1261 accgtacacc agtccccagc gcaccttggg cagcgtcaca cggaagaagg tgatccagcc
     1321 acccgctccg agcgtcatgg cggcctcctc ctcgtcgttg ccgacctgct gcatgagggg
     1381 aatcagctcg cgtgccacat aggggaaagt gacgaacatg gtggcgagca tgatgccggg
     1441 gacggcgaaa atgatgctga tgtcatgctc ctggagccag acgccgaacc agccgtgcag
     1501 gccaaacagc agcacgtaca tcattcccga gatgacgggc gagacggcca gcggcaggtc
     1561 gatgatggtg atgaggaagc tttttccctt gaattcgaac ttggcgatgg cccaggaggc
     1621 tgcgagcccg aataccgtgt tgatcggcac cacgacgagc gcggtgagca gcgtgagtcg
     1681 gatggcggcg gtcgtgtcgg ggtcggcgaa ggtttgcaca taagctcgcc agccatgctc
     1741 cagagccatg acgaacacag tggcgagcgg cgcgaacagc agggccgcga ggaacaccag
     1801 cgacacgctg atgaacagcc agcgcaccag cgaggcgagg agcgtgtcgc ggcccggccg
     1861 ggtgtccaga tcctccaggc ccagggtgtg ggcgcctgca gccatcagcg ggacaccgca
     1921 acgatccgcc gccggcccca ggcctgcagc agattgatgg tgagcagcac gacgaacgag
     1981 atcacgagca tgacgaagcc gagggccgcg gctcctttgt agtcgaactc ctccagatgg
     2041 atgacgatca gcaacggggt gatctcggtt ttcatcggca ggttgccggc aatgaagatc
     2101 accgagccgt attcgccgac agcgcgcgcg aacgcgagcg tgaatccggt cagcagtgcg
     2161 ggcagcaccg tcggcagaat gattcgccgg aaaacgtaga gccagccggc tccgagcgtc
     2221 tcggccgcat cctccaggtc gcgctgcact tcgagaagtg ccggctgcac tgagcgcacc
     2281 gcgaatggca agccgatgag tgtcagggcg acggtgaccc cgatccaggt gtaggcaacc
     2341 ttgataccca aggggtgaag gtaggagcct atccagccgt tctcggcaaa cacggtggtc
     2401 aatgctatgc ccgaaacggc cgtgggcagc gcgaaaggca tgtcgatcag cgcgtcgatc
     2461 aacttgcgcc ccggaaactc gtagcgcacg agggtccatg cgatgatgaa tccgaacacc
     2521 aggttgatga aagcggccag catcgaagcg ccgaacgaga gccggtacga gccgagcgcg
     2581 cgcgggctga gcacgattcg cacgaactcg ctccagtgca tggaggcggt catgagcacc
     2641 agagcggcca gcggaatcag cacgatagtg ctgagaaaca gcgtcgtgaa gccgaaagtg
     2701 agcgagaagc cggggagtac gctcggctgt cggaaggtgc tcacgaggtc acctccgcca
     2761 gcgcgctttc atgctggcga tgcacgatca gcatgggcca ggctgccgag gagagcagcg
     2821 agccgaatct cgccgcgagt ccggcggttt cctggatccc gaggatgagc atctgacccg
     2881 ccgactcggc aaggtgccgt gcgagctcgc tggaagcctc gccgaaacgc agctcggtcc
     2941 gtatctccag cccatgcagg gcctgtgcct ccgagcgggc atcgagcagc gcgcgcatgc
     3001 cgtgaggtcg ctgtgccatg ggggtctgct cgggcaggat gtccacgtag acggcctccg
     3061 cggaaagatg tcggaggatg cttgccgata cggcgagcgt ggcacgcctc agcgactgat
     3121 ccgcccagtg gatgagaact cgctgcgggg ccgatgcgtg ttgcggcagg atcagaatct
     3181 cctcggctcc gcgctcgagc agccattgca cgtggaacac cgcatctgca tgggaggcaa
     3241 tgaccgtcgt gccggcgaag ttggatcgcc cctcggcccc ggcgccgggc attcctagcc
     3301 ttggctcgac acgggccgaa atccgggtct tcatgcgcag ggcgagctct gagagcagcg
     3361 gctcccgcaa cagcgcttgc gcaagtcctt cggtggaggc gcaggcggta aagctcgaca
     3421 gcggtgtggg caggatgtgc acccgacgga ctccgatgac gacctgctct cccggccgga
     3481 tgcggaaggc acgttgctcg tgctgagtac gcgtcgcctc gagcagggca ctgcctgagc
     3541 catcgctcga gagcgcagcg ccatcagcgc tctcatcgat ccggatgcgg atgcgctcga
     3601 gagccccggt gaaaagcaac tcgtcgaccg cgccgcgtgt caggtagttg gaggtgagac
     3661 gctcgcgcgt cggtgcgatc tcgatctcct ccgggcgcac caccgcaacg acctcgtgct
     3721 cacggtcggc gtgaaagggg cttggcagac tcgcgctggc ctcgaccggg gtctgtccga
     3781 agcgaattcc gttcttcgac tgtcgcgcaa gaatcagatt ggcggcaccc aggaaagtgg
     3841 cgacgaagcg cgtagccgga cgggtgtaca gctcgtgggg tcgcccgagc tccagcaatc
     3901 gccccagatt catgactccg atccgatcgg cgagcgcgaa cgcctcttcc tgatcgtgcg
     3961 tcacgaggac agtcgtgatc ccgagctcgc gctgcacctg gcggatcgtg cgccgcagct
     4021 cctcgcggat cttcgcatcc agagccccga agggctcatc cagcagcagc acttccggct
     4081 tgtgagccag cgcgcgcgcc actgcgaccc gctgctgctg gcctcctgag agctgcgcgg
     4141 gcaggcgctc gtccatgccc tcgagggcta cgagtcgcag cagctcctgg cggcgcctgc
     4201 ggcgatcggc cgcgcgcatg cggcgcacgc gcaaggcaaa ctcgatgttg tccgcgactg
     4261 tcatgtggcg aaagagcgcg tagttctgga agacgagccc cacaccccgt tcgcgcgctg
     4321 caacatgggt cacatcacgt ccgtgcagtg agatgcggcc gtggtccact ccggcaaggc
     4381 cggcgatcgc ccgcagcaag gtgctcttgc cgctgccgct cgggccgagc agcacgaaga
     4441 attcgccgtc cccgatgtcc agtgagacat cgttgacgac cggcacgccc tgatagcgct
     4501 tggtgacctg gtcgagggtg atcgacatgt tcttgaaatt ctgcggcctc gagtaacccc
     4561 atgctagcgg ggcgcactcc gcgccgtcag ttatacggag gtcaccggcc ggttacaaag
     4621 tggttacaga ccgggccagc gggccgtatc ggctcaggcg agattgcagc cgggcagcag
     4681 cctcagatga tggtcagcac accttcgcag gccttcagat ccttgatcaa gccgatgaca
     4741 tggtcgctgc tctcagcaac gatgacgtag gagatcgaga ggaaattgcc gttttcgctg
     4801 agccgctcgc tgacgcgggt ggggtcgagc ccgggcacgt ggcgcagcat gatggcccgg
     4861 acccgcttgc ggaactcctc ggacggtctg cccacgattt tgagcggata gtcggtggga
     4921 aaccgcagca gcggcggcgg ctctaccacg gccgatccgc gagctcgcgc ttgtagcgtt
     4981 gcagctcgtc gaaaacacgg cgccagagag gtcccggctt tccatcgcca accggcttgc
     5041 cgtcaatgcg cgtcacgggt tgcacctcgc gagtggccga cgaaagccaa acctccgcgg
     5101 cgttacgcag ctcggcttcg ctcaccggcg cagctgtgta ggcgatgccg gcgcgctcgg
     5161 ccatttcctc cactgcgctc cgggtcgtgc ctggaaggat cttgcgcgag ttcggcggcg
     5221 cgcggaccac accatcgatg accacctgga cggctgatga ggaggcttcc atcagctcgc
     5281 cctccctgag cagaatggtc tccccggcac ccgcatccac cgcaagctga cgtaacagta
     5341 cattggcgag aagggcgacg gacttgatgt cgcactgtgc ccagcgtgtg tcctccgcag
     5401 tcacgcacgc gaggccgctc tccagagttg ctgcggctat cggtgggagt ggggcgcaga
     5461 aggcaaagac cgttctgggt atcagaggca gcggagcgtg attgcgaccc ttctcggcac
     5521 cacgtgtaac ctgccagtaa acatactgct ggcccccgcc gttgcgtgcg atcagcgttc
     5581 ccagcagctc gcgccactcc tgccgggtgt gcggatcgtc catgcgcacc tgcttcaggc
     5641 tgcgcgtcaa tctctcggca tgtgcctcga agcgaaacgg tcgcccgcca tagacgggca
     5701 taacttcata cacgccatcg ccataaagaa aaccacgatc gagaggcgaa atgcgcgcct
     5761 ccgcgagtgg caggaaagca ccattgagat aagcgagtgg gagaggctcg gccatgagtt
     5821 caattgaacc acagcgcgct gcagagcatc cagcgcctca ggtgacatcg gcgacggcgc
     5881 cggctgagtg gccgccgaga gtcaatccgg acagcctcac ccgagagcgc ccggctgaag
     5941 agtgcgcttg cgttctggcc tcgatgccgc tacggcgtgc tgtgccgcag gtcaaaagcg
     6001 gccgctgtcc acatcctgaa tgaatctgat caggccgctc atgtaggtct tctggtcgtc
     6061 atagagcgcc agatgactac cgttcggaca atagagataa cgtcccttgt gcacactgcg
     6121 cgcgatccgc tccatctgcg caggttccat ggtgtcgtag cgggctccga ttgccagcgt
     6181 gggcaccgtg atcctgggca ggtcggccgt gcggtcccac ttctccagct tgccgctcgc
     6241 gcccatctca cttgggccct gcatgggcac gtagagtttc ctgttggttt ttctgaatgc
     6301 gcgtaaaacg gggtcgggcc attggtcagg gggcatgcgc agcacgtggt acacgtagaa
     6361 attctgcgtc aacagctcct cgtaacgggg gtcttcgtag cgcccggcgg cctccagccg
     6421 caggatctcg gcgagtgcct tctgatccat ggccggcatc agcaccttgt gcgcatagtc
     6481 gttgtacgcc gggatgctgg ccaccatgtt ggagatgatc aggcccttga gattctgctg
     6541 atacttcaac gcatactcga gcgccagcat gccgccccag gattggccgt acagatagaa
     6601 gttgctgcgg tccaacttca gcgccttgcg tacctgctcg acctcttcga cgaagcgggg
     6661 caggtcccac agcgtgggct catcgggctg gtcgctgtaa gccgatccta actggtcgta
     6721 gtagtagtac tcgatgccgg cgcctgggaa atagctgtcc agggcctcca ggtactcgtg
     6781 agttgcaccg ggaccgccgt gcagagtgag caccttgatg gaggggttgt tgccgacgcg
     6841 cttggtccag acgtgaaaag gacccttggg tgtctggatc gtgatgatct tgacgccgcc
     6901 gctcagaaca tcatcacggc ccgacgaatc gaagtagctg ccgggagccc ccctaccgga
     6961 ggccgcctgc aaggatgcgc aggcgcacag cgtcgcaacc aacgcaagtt tactgacgat
     7021 ggatcgcatc ggttgacccc ctgaatgctt cgagtcgtcc tgctcagtat cgcgtcacga
     7081 aggacatcag atggtaaagg atagcgacgt agagcagcac cagggagcac agtgcgagga
     7141 tgctggtcca gatcagggcg ccgagcggtc gtgccgtcct ccaggcgacc cagaccgagt
     7201 acagcatcgc cgccgtcccc ccgatgtaga ccacgacgga cagcagatac atcagcagaa
     7261 gaagcggatc gagcgaggcg gtgaggacgg ccaggctctt cgtcccggag atcagcaggg
     7321 tgagccatgc gaggctcatg acgaccaggg cgatggctgc cagacgcgag atcaggtggc
     7381 cgcgccatgc gagccccgtg accggtaggc ggaagttgtg tcgtcgccgc gagatggccg
     7441 cgatcggcca gagcgccccg gtcagcacca gcgcgcacag ggccagcacg atggccggga
     7501 ccagccatgc aggcgagcgc cagccgggag tcggatcgaa caccatgaaa ggtgagagct
     7561 catccatgct cacgcggacc actcgaccgt cgacgacctt ggcagccatc cgccagccgc
     7621 tggccgcatc gcgccacacg aaaggtgcca cttcctgata gtgccgcaac actccattac
     7681 gccccatcat gagtgacatc gtgagcgtgc cgtccttcgc ggtggccacc ttggctggcc
     7741 cgagaatctg gatcaggctc atgaaggagc ggtcggcgcg gcgcgaatca tcgtagtagc
     7801 cgaccatcat gtgcgcatgc tgggcggcga tgtccttcgg cacgctaccc atgggccatg
     7861 ggcccggaaa gtaacgatcg acgaactcgt cgaacagcgc ctggcgcacc tggcccgcag
     7921 cgccctgacg accctgactg ttgaaagaca tgaacagccc gacgtggtca tccagaaaca
     7981 gatgcagata gctatgcatc cagaccgtgt caccgccgtg actgatgatg cgatgaccgt
     8041 tgtaattctg ctcgtagaat cccaggtcca tgcgattgag gggcggcacc atggtcagtg
     8101 cagtggtgtg catctcttca gcggtctgcg ccttcaggat ccgttgacca tggaattcgc
     8161 cgtcctgcag gtgtgcgatc atgaacttcg ccatgtcctc accgcttgcc gcgagactgc
     8221 ctgcaggggc gggcacgacg tattcgtagg gcttttccgg ctcgctggcg accctgtagc
     8281 cttggctcac ttccggcagc agcgtggcgg gcagcggctg gcggaagctc gcgtgcgtca
     8341 tgcccagcgg cttgaagata tgttcgtcga tgtagtcatc gaagctctgg ccggagaccc
     8401 gctgcacgag atagcccgcc accgcggtgg cgtagttgga ataagcgggc gtcgagcctg
     8461 ccttgaagat ccgggtgggc gtgaagtcgc gggcgtactg agccaggggg acgacggcct
     8521 tcggattgtc cgcgatgagc gatttgatct gctcctcgaa tccgggggtg tgcgtcatga
     8581 catcacgcag cgtgatcggt ccgtccggcc ggtcgggtat gtggaagtcg aggtagtcat
     8641 tgatattgcg atcgaggtcg agcttgccct gctccaccat ctgcatgacc gcggtccagg
     8701 tgaacagctt cgagaccgag ccgggccgga acagggtgcg ctccggatcc acaggggtgt
     8761 ggtgcgcgac atccgaatag ccatagccct tcgcgaagat gagctcgccg tccttcacga
     8821 ccgttacgac cgcaccggca atgtcaccat tctcgatggc atagggcatg aacccgtcca
     8881 accaggcctc gaggtccgtc ttatcgagcg tatgagcggt cgtgtaggga ttcggaatcg
     8941 ggccgcccga ggcatcgccg atggcggagg agctggcagc ggcaggggcc ggggacgcag
     9001 gactcacact gggcccgggc ggcgaagagg gcgccgcaga ggggcttgcc gcgccctgtt
     9061 gagcaatcgc ggcaccggac aagaaccctg ctatcaatag accccaggca aaaacgcttc
     9121 gcatgacttc cccccctgat gcgttattga cggttccgtt tccgcaacta tactgctttt
     9181 aagcaacgag ccatcccaat atgagaacaa gtctctcgcg ccagcgcaag ctcacctccc
     9241 ccagccccct gcgattgcat gcgccagtgt cggcctcatc agctcgcgag cgacgatgag
     9301 ccgccccctc gtgcagttcc gggtgccgtt cgtcgttttc ctcggcgatc tagccgatga
     9361 aaccttctgc aagaccggaa tggggctggt gcaatggagg cgccaggagt gcgtcgggca
     9421 gattcgtctg cccggctgtg gggcggatgc tgaagtaccc gacctcgatg tttcgcaggc
     9481 caaggcggcg ggtgcccgga gcctgatagt cgggtcggcg gtcgttgggg gaggactgcc
     9541 ggaaggctgg atcgatgtgc tgtgcgaggc ggcgcggtcc ggcattgaca tcgttgcggg
     9601 gctgcacgtg cggctggcga ccctgcccgg attgcgcgag gcggccgagg cgggcggtgc
     9661 ccgactgatc gacgttcgtg tcccgccttc cggcctgccc gtggggacgg gccagaagcg
     9721 ctcgggccgc agactcctga cgatgggcac cgactgcgcc tgcggaaaga agtacaccgc
     9781 gctggcgctc gacgcggagc tccgacgccg cggtgtcgag agctcgtttc gagctactgg
     9841 gcagaccggc atcatgatcg cgggggaagg gattccgctc gatgcggtgg tggcggactt
     9901 cgtgacgggc gccgcagagc ttctctcgcc ggcggcatcg ccaggccatt gggatgtgat
     9961 cgaggggcag gggagcttgt ttcaccccgg ctacctgcag gtgaccgtcg gattgctcgt
    10021 aggctcccag cctgatgcct tcgtcgtgtg ccacgatcca gtgcgggaat cgatctcggg
    10081 gtggccggat ttcccgctgc cgactcctgc acaggtcatt gcgcgaaccg tggaactcgg
    10141 tcgcatgacc aacccggcta ttcgctgcgt cggtatctcg ctcaatctgt ccaaggtgcg
    10201 gcccgaagat cgggctcgcg tgctggagca gtatgccgtg cagaccggct tgccctgttg
    10261 tgatccattg ctcggcgggg ttggccccat cgtcgagagc ttactgaggg cctttcctgc
    10321 ctgagtgcca gccgatgacc aagacacagc tctctac
//
LOCUS       DASRHN010000103         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1188928, whole genome shotgun sequence.
ACCESSION   DASRHN010000103 DASRHN010000000
VERSION     DASRHN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188928"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..436
                     /locus_tag="VFB37_03980"
     CDS             59..436
                     /locus_tag="VFB37_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZO21640.1"
                     /translation="MTGFVLRAVISALGLWLATRLVWGIRIDDAASLVLAGLLLGVVN
                     AVIRPIAIILTLPLTILTLGLFLLVVNTGMVALVAAILPGFHIYGGFRAAFLTALIVG
                     LTGWIGSYLIGPRGKIDVYVRKL"
     gene            complement(423..1253)
                     /locus_tag="VFB37_03985"
     CDS             complement(423..1253)
                     /locus_tag="VFB37_03985"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZO21641.1"
                     /translation="MEARPLEGCTIAIPETRAAEVFAALLERRGATVLRCPLVSIRDA
                     PDPGPILQWVRALIRGECDDLILMTGEGLQRILDCLDRHEPALREDFVRSLDQVRRIT
                     RGPKPARALRELGLKTDIAAEPPTTEGVIASLRSQDLRGRRVGLQLYGTVPNAPLLEF
                     LESAGAHVLPVAPYVYADAADDQAVLDLLEKLRSGEVDAIAFTSTPQVERLFAVAPED
                     SVISALGKTLVAAVGPVVAQSLSRHGVQARVMPEESFFLKPLTSVLESALAERRLRAS
                     "
     gene            complement(1267..2757)
                     /locus_tag="VFB37_03990"
     CDS             complement(1267..2757)
                     /locus_tag="VFB37_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HZO21642.1"
                     /translation="MSQSAESAARAVAAPTEIFGQPRGLATLFLTEMWERFTYYGMRA
                     ILILFMTGAVTSGGLAIGDKAASSIYGLYMACTYLLSLLGGWIADRLIGAQQAVFCGA
                     VFIMVGNAMLASGHTQVFFLGLLVAVFGVGLLKPNVSAIVASLYPEGGARRDAGFSIF
                     YVGINIGSFMGSWLVPVFAARYGWHWGFALPAVGMLIGITQFNLTRRHLGSAGASAAP
                     GARGSWIPVIVFVALVVFVAALSVSGHLQIDPIAVSAAATWLILIIAISYFAYLFFFA
                     GLSDIERRRAYLMAVLSIGCATFWAGYEQMGNSFNLFAQRYTDLDIIGIHIPAGVFQA
                     VNPFFILLFAPLLASLWVRLDRRGSDPSAPAKFGVGLILMGLGFLVMYCASQYVLAGQ
                     KVLPWWLVATYLLHTLGELCLSPVGLSSMTKLAPARFVGQVMGVWFMATALGENLAGQ
                     LSAQYDASQLTSLPTLFMKIFLWGTLSGGVMLALTPLLKRLMSGAR"
     gene            complement(2759..3523)
                     /gene="tatC"
                     /locus_tag="VFB37_03995"
     CDS             complement(2759..3523)
                     /gene="tatC"
                     /locus_tag="VFB37_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZO21643.1"
                     /translation="MSEEPEKLAEGTLISHLLELRDRLIRALAVVGLVFIPCALNSNQ
                     IFTFVATPLKDKLPKGTQLIATTVMSPFLTPFKLSLFVAVFIAMPYIIYQLWAFVAPG
                     LYRHEKRFAVPLLVSSIILFYVGVFFAYFAVFPVMFQFFANTTPKGVAMMTDISAYLD
                     FVLTMSLAFGAAFEVPIAVVLLVLTGIVRIEKLKQNRGYVLLGIFILAAFLTPPDAIS
                     QSIMAVPMYALYEGGILMARILSRMRQSSGHEEASA"
     gene            complement(3507..>3715)
                     /locus_tag="VFB37_04000"
     CDS             complement(3507..>3715)
                     /locus_tag="VFB37_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21644.1"
                     /translation="ELPKSYPDTYSHAHPTDSEGRPVPAASPAPETAAADPRQQDWVG
                     GVSGDSSMPSAADAARAQGHERGT"
BASE COUNT          738 a         1142 c         1235 g          600 t
ORIGIN      
        1 agcaggcgat cacgcggctg aaagttctgg tcgaaaggca gctcgggggg cccgcgtcat
       61 gacaggattc gtactgcgtg cagtcatcag cgcgctcggc ctctggcttg ccacacggct
      121 cgtgtggggc attcgcatcg atgacgccgc ttccctggtg cttgcgggcc tgctgctcgg
      181 tgtagtgaac gcggtgatca ggccgatcgc aatcattctg accctgccgc tcaccatcct
      241 gacactgggc ctgtttctgc tcgtggtgaa taccggcatg gtggcgctgg tcgctgcaat
      301 cctgccaggg tttcatatct acggcggctt ccgcgccgcc tttctgactg ccttgatcgt
      361 cggcctgacc ggctggatcg gctcctacct catcggtccg cgcggcaaga tcgacgtgta
      421 cgtcaggaag ctctgagacg cctttcggcg agtgcgctct cgagcacact ggtcagcggc
      481 ttgaggaaga aggattcttc gggcatgaca cgtgcctgaa ccccgtgacg gctgagcgac
      541 tgggccacga ctggtccgac tgcggcgacg agggtcttgc ccagtgccga aatgacagaa
      601 tcctctggcg ccaccgcgaa cagccgctcc acctgcggtg tgctggtgaa ggcaatcgcg
      661 tccacttcgc cggagcgaag cttctcgagc aggtccaaca cggcctgatc atctgcggcg
      721 tccgcgtaca cgtagggagc cacgggcaga acatgtgcgc cagcgctttc cagaaactcg
      781 agtagcggcg cgttcggaac ggtcccataa agctggaggc ccacgcgtcg gccacgcaga
      841 tcctgtgagc gcaggctcgc gatcacgcct tccgtggtcg ggggttcggc tgcgatatcc
      901 gtcttcaggc cgagctcgcg cagagcccgg gcgggctttg gcccccgggt gatgcggcgc
      961 acctggtcga gcgagcgcac gaaatcctcg cgcagtgcgg gctcgtgccg atccagacaa
     1021 tcgagtattc gctgcaggcc ctcgccggtc atcaggatga ggtcatcgca ttcaccgcga
     1081 atgagtgctc gcacccactg caggatgggg cccggatcgg gcgcgtcccg gatcgagacc
     1141 agagggcatc gcaggacggt ggcgccgcgg cgctcgagca gggcggcgaa gacctcggcg
     1201 gcccgagtct ccgggattgc gatggtgcaa ccctcgagtg gccgtgcttc catcgaccca
     1261 gggcctctac cgcgcgcctg acatcagacg cttcagcagc ggtgtcaggg ctagcatcac
     1321 gcccccggag agcgtgcccc agaggaagat tttcatgaac agtgtcggca gcgaggtgag
     1381 ctggcttgcg tcgtattgcg ccgaaagctg gccggccaga ttctcaccga gggccgtggc
     1441 catgaaccaa acgcccatca cctggcccac gaatcgtgcc ggggcaagtt tggtcatgga
     1501 cgacaggccc acgggagaca ggcatagctc gcccagtgtg tgcagcaggt aggttgcgac
     1561 aagccaccag ggcagcacct tctgtcccgc gagcacgtac tgtgaggcgc agtacatgac
     1621 caggaatccc agtcccatca gtatcaggcc gacgccgaat ttcgccgggg ccgacggatc
     1681 ggacccccgc ctgtcgagtc gtacccaaag tgatgccagc aggggcgcga agagcaggat
     1741 gaagaaggga ttgaccgcct gaaacactcc ggcggggatg tgaatcccga tgatgtccag
     1801 gtcggtatag cgctgcgcga acaggttgaa ggagttgccc atctgctcgt agcccgccca
     1861 gaacgtggcg catccgatgg aaagaaccgc catgaggtaa gcgcggcggc gttcgatgtc
     1921 cgacaggccg gcaaagaaga acaggtaggc gaagtagctg atcgcgatga tcaggatgag
     1981 ccaggtggcc gccgcggata cggcgatggg atcgatctgc aggtgaccgc tgacactcag
     2041 cgcagcaacg aaaaccacca gcgcgacgaa cacgatgacg gggatccagg atccccgcgc
     2101 tcccggcgcg gccgatgccc ccgcgctccc gagatgccgg cgcgtgaggt tgaactgggt
     2161 gatcccgatc agcattccga ccgcgggcag cgcgaatccc cagtgccagc cataacgggc
     2221 cgcaaacacc ggcacgagcc aggagcccat gaaggatccg atgttgattc ccacgtagaa
     2281 gatcgaaaag ccggcatcgc ggcgcgcgcc gccttcggga tacaaggagg cgacgatcgc
     2341 gctcacgttc ggtttcagca ggccgacgcc gaagaccgcc acgaggagcc cgaggaagaa
     2401 cacctgcgtg tggcccgaag ccagcatggc gttacccacc atgatgaata cggcgccaca
     2461 gaatacggcc tgctgcgcgc cgatcagccg gtcggcaatc cagcctccga gcagcgacag
     2521 cagataagtg caggccatgt agaggccata gatggagctt gcggctttgt caccgattgc
     2581 caggccgccg ctggtcaccg cacccgtcat gaacagaatg aggatggcgc gcatcccgta
     2641 gtaggtgaag cgctcccaca tctcggtcag aaacagggtc gcgagtccgc gcggctggcc
     2701 gaagatctcc gtgggcgctg cgacggctcg ggccgccgac tcggccgatt ggctcatctc
     2761 aagcgctcgc ctcttcgtgt ccggatgact ggcgcatccg cgacagaatg cgcgccatca
     2821 ggatgccgcc ttcgtagagc gcgtacatgg gcaccgccat gatcgactgc gagatggcat
     2881 cgggcggggt caggaacgcg gccaatatga aaattcccag caggacgtag ccgcgattct
     2941 gcttcaattt ctcgatgcgc acgatgccgg tgagcaccag cagcaccacc gcgatcggca
     3001 cctcgaatgc tgcgccgaag gcgagcgaca tggtgagcac gaaatcgagg taggcggaaa
     3061 tgtccgtcat catcgcgacg cccttgggcg tggtattggc gaagaactgg aacatcaccg
     3121 ggaagaccgc gaagtaggcg aagaacacgc cgacgtagaa caggatgatc gaggagacga
     3181 gcagcggcac cgcgaagcgc ttctcgtgcc ggtagaggcc gggcgcgacg aacgcccaga
     3241 gctggtaaat gatgtagggc atcgcgatga acaccgccac gaacaaggag agcttgaagg
     3301 gcgtcaggaa gggcgacatc accgtcgtcg cgatgagctg cgtgcccttg ggaagcttgt
     3361 ctttgagcgg tgtggcgacg aaggtgaaga tctggttgga attgagcgcg caagggatga
     3421 ataccagccc gacgaccgcc agagcgcgga tgagccgatc gcgcagttcc agcagatgcg
     3481 agatcagcgt gccttcggcg agcttttcag gttcctcgct catggccctg agcacgcgcc
     3541 gcatccgcag cgctcggcat ggaggaatcc ccgctcaccc cgcccaccca gtcctgctga
     3601 cgcgggtccg cggcggcagt ctcgggcgct gggctggcgg caggtaccgg gcggccctcg
     3661 gaatccgtgg ggtgcgcgtg ggagtaggtg tcaggataag acttgggcag ctcag
//
LOCUS       DASRHN010000104         4761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2178233, whole genome shotgun sequence.
ACCESSION   DASRHN010000104 DASRHN010000000
VERSION     DASRHN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178233"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1661)
                     /locus_tag="VFB37_04005"
     CDS             complement(309..1661)
                     /locus_tag="VFB37_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21645.1"
                     /translation="MRDAKDRGQQNRPRSTDLTTSPAFPARGYAWTVVAILVATAVLS
                     YTDRQVLSLLVDPVRAELHISDTQISLLLGSAFAVVYGIAGIPLGYLADRASRRNLIF
                     AGVLVWSVGTLACGFARSFSELFMARIVVGLGEAVLSPAAISLIADYFPPARRGLAVG
                     AFLSGIAMGLGLSIVIGGGVLRLVEAGTFLGTPLQSLSAWRLVLVLIGAPGLLWALVI
                     LMIREPQRRPEGGVSRVAEDHPPTGLMRVLARAAPLYLIVAMASLVDNAVGAWAPSLL
                     IREFNRDAATVGMQLGLLITAGFGGGVLIGGWLADRAALSGDYARKLLVCLLAGAMIL
                     PVAASLNGSALSLVILAIPAYFALSGIVTACGFSSILDVVPPHSRAFAMAISFFLNVA
                     VGAGIGPTAVALTHERLFTAPGGLGASIAVTIGWGYLIVIAAAMIARRLSRPMAAALR
                     "
     gene            complement(1759..4107)
                     /locus_tag="VFB37_04010"
     CDS             complement(1759..4107)
                     /locus_tag="VFB37_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO21646.1"
                     /translation="MRSQVGSDQGCGPGRVRRWLQVGVVVGVALIGVAPMPAAMAAAA
                     PTADENATANDSANVLQEVVVTAEKREATVQDTPISITAITGADLQARGVSDLVSVAQ
                     QVPGVSFKTSGPGQTELEMRGLTSTGGESPTVGFYLDETSLTPPAMAQNGKVVIDPNL
                     FDLNRVEVLRGPQGTLYGAGSMGGTIKLVTNTPDPRQLGANVELIGSGTDGGGFNHTE
                     NGMLNLPLVQDYAAVRLVLTDKHISGWIDRDVLFPFPTEVDNSTQRGNVAAAPVVQRF
                     SESNSEELKGGRVSLLVQPSERLSVTAGFLYQRITQDGPNTIDVPPGNEVHYQPFDVS
                     EPFKDTFNLGDLTIKYDFDSFQVVSASSTWTRQENQTQDISEAMQDYIGGFLGPPANF
                     SFYTADGGLGPGSISEDDYTRQVSEELRVASTGNTALQWLLGGYYSSFHATSHVFSFY
                     DGFAPLFGTNNLADNHRLLDIDQYALFGEVSYLLPANLKATAGARYFLYHSDSATSVS
                     GISANGTSATLFGGAQNSGATPKLSLAYLPDERTNLYATIAKGFRPGGPNSPIPPPCT
                     PAPKQFGPDSVWSYEMGEKLKMLDSRLSLNSDVYYEDWSNLQQQVAPGCGYKFTTNAG
                     KARVYGAEMEAAVVIVPGLLFTQNFGYTHATNATTVKEAGVTSGDRLLDVPEVTANTS
                     LTYRHLLVNNLNLMMRVNNSYIDSIQDITFTRNTLPSYDLTDARIGIESDRWSVAAFV
                     DNLANKKALLSDTGALSANISILNRVATNQPRTIGADLTYRF"
     gene            complement(4104..>4761)
                     /locus_tag="VFB37_04015"
     CDS             complement(4104..>4761)
                     /locus_tag="VFB37_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO21647.1"
                     /translation="YTETALELERLRKSGKIAHLGVTNFDVPHLQELLDAGIPILTNQ
                     VQYSLLDDRPGRGMVELCRDRNIALLCYGTVCGGFLSERWLHRPEPEAGLANRSLVKY
                     KLIIDDFGGWELLQRLLVTLARIATEHRTDIASVATRAILDRPQVAAAIVGATNTSHL
                     IAHGHIEALRLSVADLGAIASVTAQRHGPLGEVYGLERDRTGPHGRIMKYELNTGGAQ
                     "
BASE COUNT          940 a         1515 c         1489 g          817 t
ORIGIN      
        1 ttcgccggca cgataccgtc tactggcgcg ctccccactg gcgaggacac atccggatgg
       61 ggcacatccg gacgcggacc gtgcaactgc aacttcccgc gtgatactca ggccaggagg
      121 aagggtgcaa gccgatcgcg ataccgccgg ctcagcgtga tacgcgttcc acgcctgagg
      181 acgagttcat agtccatgaa acagccgatg catctccgct atgtgctcga cgatcgtcga
      241 acggtggacg cggacgaaca ggctgtcgct cttgcacgag ggttccaccc gtgagaggaa
      301 ccccgcgctt accgtaacgc tgcggccatc ggcctggaca ggcgacgcgc gatcattgcc
      361 gcggcaatga caatgagata gccccagccg atggtcacgg cgatggaggc gccgagtccc
      421 cccggcgcgg taaacagccg ctcatgcgtc agggccaccg cggtcgggcc gataccggcc
      481 cccacggcca cattgagaaa aaagctgatg gccatggcga aggcgcgcga atgaggcggc
      541 acgacatcca ggatgcttga gaagccgcag gcggtgacga tgcccgacaa cgcgaagtac
      601 gccgggattg ccaggatgac cagcgacaaa gcactgccat tcagagatgc tgccacgggc
      661 agaatcatag ccccggccag gagacacacc agcagcttgc gcgcatagtc tccggagagc
      721 gccgcacgat cggccagcca gccgccgatc agcacgccac ccccaaaacc agccgtgatc
      781 agcaggccga gctgcatgcc gaccgtggcg gcatcgcgat tgaattcgcg gatcaacagc
      841 gagggcgccc aagcgccgac tgcgttatcg accagtgaag ccatggccac gatcagatac
      901 agcggcgcag cgcgtgccag gacgcgcatg agccccgttg gggggtggtc ctcagcgaca
      961 cggctcacac cgccctcggg ccggcgctgc ggctcacgga tcatcaggat aaccaacgcc
     1021 cagagcagtc ccggagctcc gatcagcacc aacaccaagc gccaggctga cagcgactgc
     1081 aagggggttc cgagaaaggt cccggcctcg accagccgca gtaccccgcc accgatgacg
     1141 atcgagaggc cgagacccat agcgatgccg ctgaggaatg cccccacagc tagcccgcga
     1201 cgtgcggggg ggaagtaatc agcgatgagg gaaattgcgg cgggcgacag cacggcctcc
     1261 cccagtccca cgacgatgcg cgccatgaac agctcgctga agctgcgcgc gaacccacag
     1321 gcgagcgtac cgacactcca caccaggacg ccggcgaaga tgagattgcg ccgcgaagcg
     1381 cggtcggcca ggtaacccag gggaatgccg gcgatgccgt agacgacggc gaaggcactg
     1441 ccgagcagca ggctgatctg cgtatcgctg atatgcagct cggcgcgcac cggatcgacc
     1501 agcagactca gcacctgccg gtcggtgtag ctgaggacgg cggtggcgac cagaatcgcc
     1561 accaccgtcc acgcgtagcc gcgtgccgga aaagcgggcg aagtggtcag gtcagtagat
     1621 cgggggcggt tttgctgccc ccgatccttc gcatcgcgca tgaaccactc gattcaaacg
     1681 gctctcgcgg cccgaccttt gagcgggaca agcccctgca tcgctccact cgtgcccctt
     1741 cgtttgtcat cgtcgctttt agaagcggta agtcagatcg gctccgattg tgcgcggctg
     1801 gttggtcgcc acgcggttga ggatcgagat gttggccgac aatgcgccgg tgtcactcag
     1861 cagcgccttc ttgttggcta gattgtcaac gaaggcggcc accgaccagc gatcgctctc
     1921 gatgccgatg cgcgcatccg tcagatcgta cgacggcagc gtattgcgcg tgaaggtgat
     1981 gtcctggatc gaatcgatgt agctattgtt gacccgcatc atgagattga ggttgttgac
     2041 cagtagatga cgataggtca acgaggtatt ggccgtgacc tcgggaacat ccagcagccg
     2101 gtcacccgaa gtcacaccgg cttccttcac cgtcgtcgca ttggtggcat gcgtgtagcc
     2161 gaagttctga gtgaacagca gcccgggcac gatcacgact gcggcctcca tctccgcgcc
     2221 ataaacccgg gctttgcctg cattggtggt gaacttgtaa ccacatcccg gagccacctg
     2281 ctgctgcagg ttcgaccagt cctcgtaata cacgtcgctg ttcagggaga gacgcgagtc
     2341 gagcatcttc aacttctcac ccatttcgta gctccatacg ctgtcgggcc cgaattgctt
     2401 gggcgccgga gtgcagggtg gcggaatcgg cgagttggga ccccccggtc gaaatccctt
     2461 ggcaatggtc gcgtacaggt tggtgcgctc atcgggcagg tatgccaggc tgagcttggg
     2521 agtcgcgccg gaattctgcg cgccgccgaa cagcgtggca ctggtgccgt tcgccgagat
     2581 accgctgacc gacgtggcag aatcgctgtg gtacagaaag tagcgcgcac cggcagtggc
     2641 cttcagattc gcaggcagca ggtaggacac ttctccgaac agcgcatact ggtcgatatc
     2701 gagcagccgg tggttgtcgg ccaggttgtt ggtgccgaac aacggcgcga atccgtcata
     2761 gaacgagaac acgtgcgagg tcgcatggaa gctgctgtag tagccgccca gcagccactg
     2821 cagcgccgta tttccggtgg aagccacgcg caactcttcg ctcacctgcc gcgtgtaatc
     2881 gtcctcgctg atcgacccgg gcccgaggcc gccatcggcg gtatagaagg aaaaattcgc
     2941 cggggggccg agaaatccgc cgatgtaatc ctgcatcgcc tcggagatgt cctgcgtctg
     3001 gttctcctga cgggtccagg tggagctcgc cgagacgacc tggaaggaat cgaagtcgta
     3061 cttgatcgtc aggtcgccca ggttgaatgt atccttgaac ggctcggaga catcgaacgg
     3121 ctgataatgg acctcgttgc ccggtggcac atcgatcgta ttcgggccgt cctgtgtgat
     3181 gcgctgatag agaaatcctg ccgtaacgct cagccgctcc gagggctgca ccagcagact
     3241 cacccggccg cccttcagct cctccgagtt tgactcactg aagcgctgca ctaccggcgc
     3301 agcagcgaca ttgccgcgct gcgtgctgtt atcgacctcc gtggggaagg gaaagaggac
     3361 atcacggtcg atccagccgg agatgtgctt gtcggtcaat accaagcgca ccgccgcgta
     3421 gtcctgcacg agcggcaggt tgagcatgcc gttctcggtg tgattgaagc cgccaccatc
     3481 ggtacccgag ccaatgagct cgacattggc gccgagctgc cggggatccg gcgtattggt
     3541 cacgagcttg atggtgccgc ccatcgagcc ggctccatag agcgtaccct gaggcccgcg
     3601 caacacctcg acgcggttca gatcgaacag atttgggtcg atgacgacct tgccgttctg
     3661 tgccatcgcc ggcggcgtga gcgatgtctc atccagatag aacccgacgg tgggcgactc
     3721 gccgcccgtc gaggtcaatc cgcgcatctc caactcggtc tgtccgggac ccgaggtctt
     3781 gaatgacacg ccgggaacct gctgcgcaac cgataccaga tccgaaacgc cccgcgcctg
     3841 caggtcggca ccggtgatcg ccgtgatgct gatgggcgta tcctgaactg tggcttcgcg
     3901 cttctcggct gtcactacca cttcctgcag gacgttcgcg ctgtcgttgg cggtggcatt
     3961 ctcatcagca gtcggcgccg cagccgccat cgcagccggc atcggcgcga cgccgatgag
     4021 cgccacgccg acgacgacgc ccacctgcag ccagcgccgc actcggcccg ggccacaccc
     4081 ctggtcagat ccgacttgac ttctcattgt gctccccccg tattcaactc gtacttcatg
     4141 atcctgccgt gaggtcccgt gcgatcgcgc tcgagaccgt agacttcacc caacggtcca
     4201 tgccgctgtg cggtaacgct ggcaatggcg cccagatccg caacgctcag gcgtagcgct
     4261 tcaatatgcc catgcgcaat caggtggctg gtgttggttg cgccaacaat cgcggcggcc
     4321 acttgcgggc ggtcgagtat cgcgcgcgta gccaccgagg cgatgtccgt gcgatgctcg
     4381 gtggcaattc gcgcgagcgt gacgagcagc cgttgcaaca gctcccagcc accgaaatca
     4441 tcgatgatga gcttgtattt gacgagcgag cgattggcga gccccgcctc aggctcgggg
     4501 cgatgcagcc agcgctcgga gaggaatccg ccgcatacgg tgccgtagca cagtagcgcg
     4561 atgttgcggt cgcggcacag ctcgaccatg ccgcgtcccg gacggtcatc cagcagcgaa
     4621 tattgaacct gattcgtgag gatcgggatg cccgcatcca gcagctcctg cagatgcggg
     4681 acgtcgaaat tggtcacccc gagatgcgcg attttccccg acttgcgcaa ccgctcgagc
     4741 tcaagggcgg tctcggtgta a
//
LOCUS       DASRHN010000105        14104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1354582, whole genome shotgun sequence.
ACCESSION   DASRHN010000105 DASRHN010000000
VERSION     DASRHN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354582"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFB37_04020"
     CDS             <1..264
                     /locus_tag="VFB37_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21648.1"
                     /translation="VFARTILLGNRSYRFTQQINLHASGAAPKRQLDRQARGVARSGR
                     VTVGGRLGTRDVMRRVFVPDFDFVAVGIADESEGLVAAEVASL"
     gene            complement(408..815)
                     /locus_tag="VFB37_04025"
     CDS             complement(408..815)
                     /locus_tag="VFB37_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21649.1"
                     /translation="MKRAILAFLAGLVAWTLVVSLLNRALRGMLDGYAAAEPTMTFTL
                     GMMAARLTIAAVTSLLAGVVTRWVAPAGNRVAVVLGLFLLVAFIPIHASLWNHFPIWY
                     HLTFLLTLVPLVVLGSWFAARAQGAKPPVLANS"
     gene            878..1468
                     /locus_tag="VFB37_04030"
     CDS             878..1468
                     /locus_tag="VFB37_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZO21650.1"
                     /translation="MKAELIILGVLHRGDFHPYEIKRRVTNAMIECYTDVDVGTLYYA
                     VRRLAKDGLIAAVTRERVARGGIRTVYGITAKGRQRFQELLTEQFEAEGSVADTMYGA
                     MLFLHLADLPVVADLLLKKIARQNELIHKLDQIQKQIAPSLSTGGLHLMRHLSLQRRL
                     DRQWLKALLADVVAGKVRDVPDPRKLAAPSQLVTLD"
     gene            1551..2234
                     /locus_tag="VFB37_04035"
     CDS             1551..2234
                     /locus_tag="VFB37_04035"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HZO21651.1"
                     /translation="MASLIAASVFFLLIHFGVSGTRMRDGLIARMGERPYRAAFGIAS
                     LAGTIWLVIAYRHAPNIVLWGQLISLRPLAYVVVLIAFLFVVIGVTTVNPTRVGMEAK
                     LADGAAVVRGITRITRHPFLWGVALWALMHLIINGDLASLFLFGSLLLLALGGTASID
                     AKRRRSFPSQWESFARATSSVPFAAVFSGRNQLGPALSEIGGLKLLLAVVIYVVAFVI
                     HGRLGHPLT"
     gene            complement(2294..3166)
                     /locus_tag="VFB37_04040"
     CDS             complement(2294..3166)
                     /locus_tag="VFB37_04040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZO21652.1"
                     /translation="MSSPIRDVSDTAFWIAWHRALESERPDALFRDPFAARLAGERGK
                     AISEAMPTSTIVAWTVVLRTRIIDDFIAFAIESGTDTILNLGAGLDARPYRMGLPETL
                     RWIEADYPHIIDYKEDSLRAETPHCRLERVKIDLADESARRELLKRVNAEARGVLILT
                     EGVVPYLTNDAVASLAEDLREMRRAHYWIVDYFSEKALRYRQRKRINRAMQNAPFRFR
                     PGDWFAFFSRYGWKLKEVRYFIDEGQRLGRPIPLPRRLQLVMAVSRLFTPKRRLEAMR
                     QFAGYTLLEQETRH"
     gene            complement(3334..4056)
                     /locus_tag="VFB37_04045"
     CDS             complement(3334..4056)
                     /locus_tag="VFB37_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZO21653.1"
                     /translation="MSIWVGTSGYNYPEWKGSFYPQKLPTAKMLSFYAERFASVEINY
                     TFYRMPNEKILAGWSEETPERFRLTLKAPKRITHDARLRDCEENLSYFLRVAGTLGPK
                     LGALLFQLPPYFKKDLPRFDAFLQLLPPELCAAFEFRHASWLDEEVYARLKSRNLALC
                     VADGEKVDTPVEPTADYAYFRLRDEGYTADDILRWARVIREQAARCREVYVYFKHEEA
                     GKGPAFARLLLAGLGEPAPLAS"
     gene            complement(4073..4936)
                     /locus_tag="VFB37_04050"
     CDS             complement(4073..4936)
                     /locus_tag="VFB37_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO21654.1"
                     /translation="MAEGPMPSYGVGDVERVLHLSRSTIRGLIDAGFVKPERGARREY
                     RFSFRDMIVLRAARALIQAKVPRRRIHRSLQDLRRHLPETVPLSGLSICAVGDRVVVR
                     DGKTHWRPDSGQYLLGLDVILENGVLHMVDRVQATPDAALIRAAAPKSAEEWFTEALE
                     LEGADESAARGAYEHAVSADPQHVASWINWGRLLHEVGQTGEADDVYRRALDLCGPDP
                     VLMFNRGVLLEDLGRTGAALEAYQAAIEEDPSLAECHYNLARLYEALGQPQHAIRHLG
                     QYRRLLNNPGN"
     gene            complement(4947..5819)
                     /locus_tag="VFB37_04055"
     CDS             complement(4947..5819)
                     /locus_tag="VFB37_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZO21655.1"
                     /translation="MAARSIGSLTISFGLVAIPVKLYTATQSANAISFNLLHKDCGSR
                     LRQQYVCLKDGSMVERDQMVKGYEFAKDQYVTFTPQEIKALEEVGTHSVDISEFVPVE
                     SIDPVYFDKTYYLSPDKGAGKPYGLLTEALKQSQLCAVGRWAARGKAYIVLLRPVGDV
                     LTMQQLHFAGDVRSASEIDVPKADVKPAELKLAQQLISQQTSEKFDANAYQDEVRARI
                     EAEIQKKVEGKEISVSETAPAGEGKVIDLMEALRASLEKTESARASASQLGPRKAPKR
                     VEQPAKTARKASKR"
     gene            complement(5830..8610)
                     /gene="ligD"
                     /locus_tag="VFB37_04060"
     CDS             complement(5830..8610)
                     /gene="ligD"
                     /locus_tag="VFB37_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZO21656.1"
                     /translation="MKRPLDPYAAKRTFTRTPEPGAEVAPRRRGPLLFVVQKHAARRL
                     HYDFRLELDGVLKSWAVPKGPSLTAGDKRMAVEVEDHPFEYASFEGVIPAKQYGAGNV
                     IVWDCGVYSPDEDHKYSFGDREEAQARLRRELAEGKLSLFLRGEKLKGSFALVRTRQD
                     KQWLLIKHKDRFVAGGELLARSRSVLSNVVVEDAESLQSVQRTAAAHLTPSGPEENMP
                     AKLSPMLAETGSMPRSSVDWLYEPKLDGYRVLAFVLGSRVRLQSRRGIDLTAVFPDLT
                     ADLAQQAGADMILDGEIVALGADGKPSFNALQNRAQLKTAGEIATAVRESPAVLVCFD
                     LLHFAGLNVRGFSYVDRRRYLAQCLLPSARIQLMHVSPEAEPLYEAATASGFEGIIAK
                     RRNSLYEPGKRSGSWLKFKTTQTTEFVVGGYTQGKGEREPLGALLLGYWLDGKLRYAG
                     HAGSGLTEPVIAEIGRRAKGLERRTSPFAERVQLHRPTHWLAPELVAEVTFADWTPDG
                     LLRAPVFKRLRDDVSPESVTRPAEVPVQAAVSSSEAVPPRKSRPARARTAPTSKRSSP
                     ASQIEEIVGQLDNDAARVDLTVGDARIRLTNLDRAYWPADPAARAPAITKRDLIRYLA
                     RVSPYMLPHLVNRPLTMIRMPEGIYGERFFQKHWEQSLPDFVETIRVYSGHKEEEHRY
                     LLANNLPTLLWLGQSGTLEFHIWHSRAQPGPDCVSQSTDYASSLESLEESVLNYPDYL
                     VFDIDPYIYSGKEAKGDEPELNSRGFAVGRRVAFWLRTLLRQMSLDAIVKTSGKTGLH
                     VFVPIDRTVTFEAARHICETVGRHLMRAHPKDITLEWAVEKRTDKIFIDYNMNVRGKT
                     LNVAYSPRGLPGGPVSMPLTWEELEAAEVMDFTIATVPARLEKTADRWQDALSGKFSL
                     SRTLGRNPDRNT"
     gene            complement(8607..9302)
                     /locus_tag="VFB37_04065"
     CDS             complement(8607..9302)
                     /locus_tag="VFB37_04065"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZO21657.1"
                     /translation="MSGVSLPSAQPATIAVDAKRRVSALLMLPQKPSACFVFAHGAGA
                     GMQHPFMAAMASALVERDIATVRFQFPYMEAGSRRPDVPAVCHATVRAAVEEASQQCP
                     GVPLIAGGRSFGGRMTSQAQAQQPLPAVRGLAFLGFPLHPAGRPSEERAQHLQEVDLP
                     LLFLQGTRDELAELSLLQGVVGRLAPRATLKWMEDADHSFHVPARSGRKDAQVVQDLA
                     QTLTDWLPIVLSA"
     gene            9397..9840
                     /locus_tag="VFB37_04070"
     CDS             9397..9840
                     /locus_tag="VFB37_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21658.1"
                     /translation="MSGEIPEGASCYEGRCHCGGIGYSYYTRSAPDKWSVRACQCSFC
                     RAHGALSTSDPSGFVIFHARAGAPQIRYRFGQRTADFLICGDCGVYVAAVIDTDRGAF
                     TVVNINALNPRIEHLPAPQPMSYDTETPEQRIHRRMQRWTPCKRI"
     gene            9948..10826
                     /locus_tag="VFB37_04075"
     CDS             9948..10826
                     /locus_tag="VFB37_04075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZO21659.1"
                     /translation="MCDADAMDDMVEYKLRSGELSRRSFGALTLGVGLASVLPQAAGA
                     AEVKDSEVNIKTPDGTADAYFVHPAKGKHPGVLVWPDIFGLRPAFRQMAKRLSESGYA
                     VLVVNPFYRTKKAPTAPEHPDFNDPATRQALMSLAGTLNPTTTVTDAKAFIPWLQKQS
                     AVDRKRGMGTTGYCMGGPFTLRTAAEFPDQVRAGATFHGGGLVTDKPDSPHLLIPKLK
                     AQYLFAIAENDDKRQPEAKNVLREAFQNAHLPAEIEVYAGTLHGWCPPDSQVYNHDQA
                     EKAWSRQLALFDRALS"
     gene            complement(10895..11794)
                     /locus_tag="VFB37_04080"
     CDS             complement(10895..11794)
                     /locus_tag="VFB37_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZO21660.1"
                     /translation="MVIGTRTLLSVLLALAAWFTWRWWSIERTRRDVAQGSRHPHWND
                     LLLGFVTNVLDTLGIGSFAPTTAVFKLQHRPADEDIPGTLNAGHAPPTFVEALIFISA
                     VSVDPVTLIGMIGASILGAWLGGGVVARLPRRAIQIGMGVALLVSAGLFVAQNLQLMP
                     GGGDALGLQDGRLVFAIVVSFVLGALMMLGIGLYAPCLILVSLLGMNPLTAFPIMMGS
                     CAFLMPVGAVRFIQKGRYSLRAALGLALGGIPGVLIAAYVVKSLPMHWLRWLVVIVVV
                     YAAALMLVSARREPALQANRLTQ"
     gene            11989..13848
                     /locus_tag="VFB37_04085"
     CDS             11989..13848
                     /locus_tag="VFB37_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HZO21661.1"
                     /translation="MNIRLKVAALVASLFVVLGAAEIIVDRTILTPSFAELERADART
                     AMRRIDYALDATLEGLEVSATDWGNWADTYRFVQDRNAAFVQDNLTSIALRQLNANVL
                     LIVDPDGNIIESRTQDLKSDRPLTLDFAERNTLATAFPWRAHLRDGRPARGLMRTNLG
                     IMMLVSAPVLDGNGGGPVRGMVMLGRLLSEAEVARIGAQSQTQLALLPRQQGAARERL
                     VEAGTLTQVYRSFDDLYGRPIMTLRVDVPRRITGQGRAAIVYASAYLAGAAVIVLVLL
                     VIVLNRIVLEPLARITRHAVAIGEDKDMASRLDFRSQDEIGVLAREFDRMVGRVGETR
                     RQLVDQSFQAGFAELAKGVLHNLGNAMTPVGVRVARLRERLREAPVADAQQALMELAS
                     AGAAPQRRADLEEFVRLACKELAGSVQAAQEDIEVMSRQTAIVQSALAEQMRSARNEH
                     VIESVRLPELVGQSLEIVPDACRQRLVIKMEDSLKRLGSLRLARTVLRLVVQNVIINA
                     ADAVKESGKDKGLLQVEGELEREADGDKLHLLFRDNGIGIAADNLGRVFEKGFSTKSR
                     ETNHGIGLHWCANAIAALGGRIWAASEGPGRGTSVHLVIPLDQPATATAVGAA"
     gene            13856..>14104
                     /locus_tag="VFB37_04090"
     CDS             13856..>14104
                     /locus_tag="VFB37_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21662.1"
                     /translation="MKSQNRTPIRVLVADDEADVRDAYTQILLETDVSQEIAGFRELR
                     SRLFKRAAAETQKSRSQARGPTFDPVFCEHAEAAVAAVK"
BASE COUNT         2624 a         4421 c         4519 g         2540 t
ORIGIN      
        1 gtctttgcac gcacgattct cctcggcaat cgttcatatc gtttcacgca gcagatcaat
       61 ttgcacgcat cgggcgccgc gccgaagcgg cagcttgatc gccaggcgag gggagtcgcg
      121 cgctcggggc gggtcaccgt cggtgggcgg ctaggcacca gggatgtgat gcggcgtgtt
      181 ttcgtgccag atttcgattt cgtagccgtc gggattgcgg acgaaagcga agggttggtc
      241 gccgccgaag tcgcctcgct gtagcaccgt gccgccagcg gcgatcacgc gctcaatgac
      301 ttcgcccagc cgttccggtc actgcgcgat cgaggtccgc taccttgagc gcaaggtgcg
      361 acagaccaaa ggttgccgga tccatcaacc cccgcttgag cgcttcgcta gctattggcc
      421 aacacgggcg gcttggcgcc ctgcgcgcgt gcggcgaacc atgagcccag tacaaccaag
      481 gggacgagag tcagcaaaaa cgtcaggtgg taccaaatgg ggaaatgatt ccagagcgag
      541 gcgtgaattg gaatgaaagc cacgagcagg aataagccca gaaccacggc gacccggttg
      601 ccagccggcg caacccatct cgtgaccaca cccgcaagga gtgaagtgac ggcagcgatc
      661 gtcagccgcg cagccatcat ccctagggtg aaggtcattg tcggctcggc cgccgcgtat
      721 ccgtcgagca tgccgcgcag cgcgcggtta agaagtgaga cgacgagagt ccaggccaca
      781 aggccggcta gaaatgcgag gatggcgcgc ttcatggcga ttcacccgta ttagttcatt
      841 tataatagtc taattaaagt ttcaggatag tgcaactgtg aaagccgagt tgatcattct
      901 gggggtgctg catcgcggtg atttccaccc ctacgagatc aagcggcgtg tcaccaacgc
      961 aatgatcgag tgctataccg atgttgacgt gggcacgctt tactacgccg tccgccgtct
     1021 cgcgaaagac ggcctaattg cggccgttac gcgcgagcgc gtcgctcgcg gaggcatacg
     1081 gactgtttac gggatcacag ccaaaggaag gcaacgcttc caggaactgc tgaccgagca
     1141 gttcgaggcc gagggctccg tcgcggacac catgtacggc gcgatgttgt ttctgcatct
     1201 tgcggacctg ccggtggtgg ccgatctact gctcaaaaaa atcgctcggc agaacgagct
     1261 gattcacaaa ttggaccaga tacagaaaca aatcgctcca tcactgtcga ccggtgggct
     1321 gcatctgatg agacatctgt cgttgcagcg acggctggac cggcaatggt tgaaggctct
     1381 gctggcggac gtcgtagccg gcaaagtgcg cgacgtcccc gatcccagga aactcgccgc
     1441 gccaagccag ctcgtcacgc tggactagca cggggtgacg ttccggccca aaaatcctat
     1501 gctccatggg cagcggcacg ggctgacacc gctgctacgg gacggatgcc atggcctctc
     1561 tcatcgcagc gagcgttttc ttcctgctca ttcattttgg ggtttcgggt acccggatgc
     1621 gggatgggct gatcgcgcgg atgggggagc gcccctaccg ggcggccttc ggcattgcct
     1681 ctctggcagg caccatctgg ctggtcattg cctaccgaca cgccccgaac atcgtgctct
     1741 ggggacaatt gatcagcttg cgaccgctgg cctacgtcgt cgttctcatc gctttccttt
     1801 tcgtggtgat cggtgtcacg actgtcaatc caacacgcgt cggcatggaa gcgaagctgg
     1861 ctgatggggc tgctgtcgtc cgcggcatta ctcgcatcac tcgccacccg ttcctctggg
     1921 gcgtggcgct gtgggctctg atgcatctca tcatcaacgg tgacctggca tctctgttcc
     1981 tgttcggatc actcctgctc ctcgcgctcg gaggcactgc ttcgatcgat gccaagcggc
     2041 gacggagttt cccgagtcaa tgggagagct ttgcgcgagc gacctccagc gtcccattcg
     2101 ccgcggtctt ttcaggaagg aaccagctgg gtccggcatt gagtgagatc ggcgggctca
     2161 aactgctgtt ggcggtagtg atctacgtcg tggccttcgt gattcacggt cgattgggac
     2221 atccgctcac gtagaccgct ggaaccagat cgcaacagtc tccggcgcgg cagcgctgct
     2281 atcagccccg aaatcagtgg cgggtttcct gctcgagcag ggtataacct gcgaattggc
     2341 gcatggcctc caggcgccgc ttcggagtga agaggcgcga gaccgccatg acgagctgca
     2401 gacgccgtgg caaagggata gggcgcccca ggcgctggcc ttcgtcgatg aaatagcgca
     2461 cttccttcag cttccagccg tagcggctga agaaagcgaa ccagtcgccc ggccgaaagc
     2521 gaaacggcgc attctgcatc gcgcggttga tgcgtttgcg ctgccggtat ctgagcgcct
     2581 tctcggagaa ataatccacg atccagtagt gagcccggcg catctcacgc aggtcctcgg
     2641 caagtgaagc caccgcgtcg ttggtgagat acgggaccac cccctcggtc aggatcagga
     2701 cgccgcgtgc ctccgcgttc actcttttca acagctcgcg gcgcgcgctc tcatcggcca
     2761 gatcgatctt cacccgctcg aggcggcagt gcggcgtctc tgcgcgcaga gagtcttcct
     2821 tgtagtcgat gatgtgcggg tagtccgcct cgatccagcg gagcgtctcg ggcaggccca
     2881 tccggtacgg gcgtgcgtcc aagccggcac ccaggttgag gatcgtgtcg gtgccgctct
     2941 cgatggcgaa ggcgatgaag tcatcgatga tgcgggtgcg cagcaccacc gtccacgcca
     3001 cgatggtgga ggtcggcatg gcctccgaga tggccttgcc gcgctcgcct gcgagccgcg
     3061 cggcaaacgg gtcgcgaaag agcgcatcgg ggcgctctga ttcgagcgcg cgatgccagg
     3121 caatccagaa ggcggtatcc gagacgtctc tgatcggcga ggacatgcgg gcggattcta
     3181 actccgcccg ctcgctctca ccggcttgcc gcaatgtgac aaccggcgat taagattgtg
     3241 cggttgagct ttgcggcgcc aagtgaagac cgccgcactc gtccgcgggg gccgagcgca
     3301 cacagctctg caccgtcagc agcggagtgc cgcctaggag gcgagcggcg cgggctcgcc
     3361 gaggccagcc aggagcagcc gggcgaacgc cgggcccttg cctgcctctt catgcttgaa
     3421 atagacgtaa acctcgcggc agcgagctgc ctgctcgcgg atcacccgcg cccatctcag
     3481 gatgtcatcc gccgtgtacc cctcatcccg cagccggaaa taggcgtaat cagcggtcgg
     3541 ctcgacgggg gtatcgacct tctcaccgtc ggcgacgcac aatgcgagat tccgtgattt
     3601 gagccgcgca tagacctctt catccagcca tgaggcatgg cgaaactcga acgccgcgca
     3661 gagctccggt ggaagaagct gcaggaacgc atcgaaccgc ggcaggtcct tcttgaaata
     3721 gggcggcagc tggaacagca gcgcgccgag tttcggcccg agcgtgccgg cgacccgcag
     3781 gaaataactc agattctcct cacagtcgcg caagcgggca tcgtgcgtga tgcgtttggg
     3841 agccttgaga gtcagcctga agcgctccgg agtctcctcg gaccacccgg cgagaatctt
     3901 ctcgttgggc atccgataga aggtgtagtt gatctcgaca ctcgcgaatc gttcggcata
     3961 aaaagacagc atcttggcgg tcggcagctt ctgcggatag aagctccctt tccactcggg
     4021 gtagttgtag ccggacgtgc cgacccagat gctcatgcgg agggccgcag ggtcagttgc
     4081 cgggattgtt gagaagccgg cgatattgtc cgaggtgccg gatggcgtgt tggggctggc
     4141 ccagggcctc atacagccgc gccaggttgt aatggcactc ggcgagactc ggatcttcct
     4201 cgatggcggc ctggtaagcc tcgagcgcgg cgccggtgcg gcccagatcc tcgagcagta
     4261 cgccacgatt gaacatcagc acgggatcag gaccgcaaag atccagcgcg cgccgataca
     4321 catcgtcggc ttccccggtc tgacccactt catgcagcag gcggccccag ttgatccacg
     4381 aagccacatg ttgcggatct gcgctcactg catgctcgta agcgccgcgc gcggcggatt
     4441 catccgcgcc ctcgagctcc agtgcctcgg taaaccattc ttccgcgctc ttgggggccg
     4501 ccgcgcggat cagcgccgca tcaggggtag cctgaacgcg atccaccatg tgcaagacgc
     4561 cgttctcaag gatcacatcg agtcccaaca ggtactgtcc gctgtcgggt cgccagtggg
     4621 tcttgccatc gcggacgacc acccggtcgc cgactgcaca gatgctcagg cccgaaaggg
     4681 gaaccgtttc gggtagatga cgacgcaggt cctggagtga gcgatgaatg cgccgacggg
     4741 gaaccttcgc ctggatgagt gcccgcgccg cacgcaatac gatcatgtcc cggaaggaga
     4801 accggtactc gcggcgtgcg ccgcgctcgg gtttcacgaa gccggcatcg atcaggcccc
     4861 ggatcgtact gcgcgagagg tgcagaacgc gctctacatc gcccacaccg taggaaggca
     4921 tcggtccttc tgccacctgc cggaacctat cgtttgcttg cctttcgcgc agtcttggcc
     4981 ggctgctcga cacgtttcgg cgccttgcgc ggcccgagct gagaagccga agcgcgtgcg
     5041 ctctcggtct tctccagact cgcccgcagc gcttccatca gatcgatgac cttgccttcg
     5101 cctgctggcg cagtctcgga cacggagatc tccttgcctt ccaccttctt ctgaatctca
     5161 gcttcgatgc gcgcgcgcac ctcgtcctgg tatgcgttcg cgtcaaattt ctcggaggtc
     5221 tgctgactga tgagctgctg agcaagtttc agctccgcgg gcttcacgtc cgccttcggc
     5281 acatcgatct cggacgcgct gcgcacgtcg cccgcgaaat gcagctgctg catcgtcagg
     5341 acatcgccca cgggccggag taacacgatg taggctttgc cacgtgcagc ccagcggccg
     5401 accgcgcaca gttgcgattg cttcagcgct tcggtgagca gtccgtaagg cttgcccgcg
     5461 cccttgtcag gcgacaggta gtaggtcttg tcgaagtaga ccgggtcaat cgactccacc
     5521 ggcacgaact cggagatgtc caccgagtga gtaccgactt cctccagagc cttgatctcc
     5581 tgcggagtga aggtgacgta ctggtccttg gcgaactcgt agcctttcac catctggtcg
     5641 cgctccacca tgctcccatc cttcagacac acatactgct gtcgcaggcg cgagccacag
     5701 tccttgtgca gcaggttgaa ggagatcgca ttcgcggact gcgtggcggt gtagagcttc
     5761 accggtatcg cgacgagccc gaaggaaatc gtcagggatc caattgagcg cgcggccatg
     5821 atccacctgt tatgtgttgc ggtccgggtt gcgaccgagg gtccgggaca agctgaactt
     5881 gcccgacagt gcatcttgcc accggtcggc cgtcttttcc aaccgggcgg ggacggtggc
     5941 gattgtgaag tccatcacct cggctgcttc gagctcctcc caggtcaggg gcatggagac
     6001 ggggccgccc ggcaggcccc ggggcgaata ggccacgttg agggtcttgc cgcggacgtt
     6061 catgttgtaa tcgatgaaga tcttgtcggt cctcttttcc accgcccact cgagggtgat
     6121 gtccttcggg tgcgcccgca tcaggtgcct gccgaccgtc tcgcagatgt gtcttgcagc
     6181 ctcgaatgtc acggtccggt cgatggggac gaagacgtgc aggccggtct tccccgaggt
     6241 cttcacgatg gcatcgagcg acatctgccg tagcagcgtt cgcagccaga aagccacccg
     6301 gcggccgacc gcaaagcccc tggagttgag ttcgggctcg tctcccttgg cctccttgcc
     6361 ggagtagata tacgggtcga tgtcgaagac caggtagtcc gggtaattga gaactgactc
     6421 ctcgagcgac tcgagtgagc tcgcgtagtc ggtactctga ctcacacagt ccggcccagg
     6481 ttgggcgcgc gagtgccaga tgtggaactc gagcgtgcca ctctggccga gccagagcag
     6541 ggtgggcaga ttgttggcca ggagatagcg gtgctcctcc tccttgtgtc cggagtagac
     6601 ccgtatggtc tcaacgaagt ccggcaacga ctgttcccag tgcttctgga agaagcgctc
     6661 gccgtagatg ccctcgggca tgcggatcat cgtgagcggc cggttgacaa ggtgcggcag
     6721 catgtaaggc gagacgcggg ccaggtagcg gatcagatcg cgtttggtga tagcaggggc
     6781 ccgcgccgcg gggtccgccg gccagtaggc ccggtcgagg ttcgtcaggc gaatgcgcgc
     6841 atcgccgacc gtcagatcga ctcgtgcggc gtcgttgtcc agttgaccca cgatctcctc
     6901 aatctggctg gctggactcg accgcttgct ggtgggcgca gtcctggcac gggctggcct
     6961 gctcttgcgg ggtggcactg cttcgctgga ggacacggca gcttgcaccg gcacctcggc
     7021 agggcgcgtc acgctctctg gggacacatc atcccgcaac cgtttgaaca cgggcgcacg
     7081 cagcaggccg tcaggcgtcc aatcggcgaa agtcacttcc gcaaccaact ccggcgccag
     7141 ccaatgggtt ggacgatgga gttgcaccct ttcggcgaac ggcgaggtgc gtctttccag
     7201 ccccttcgcc cgcctgccga tctccgcaat gacaggttcc gtcagccccg atcctgcatg
     7261 tccggcataa cgcagcttgc cgtctagcca gtagccgagc agcagggcgc cgagcggctc
     7321 acgctcgcct ttgccttggg tgtatccgcc cacgacgaat tcggtggttt gggtcgtctt
     7381 gaacttcagc cacgagccgg agcgcttgcc cggctcgtag aggctgttgc ggcgcttggc
     7441 gatgatgcct tcgaagccgc tggcggtggc tgcttcgtaa agaggctctg cctccgggga
     7501 gacgtgcatg agctggattc gcgccgaagg cagcaggcat tgagccaggt agcgacggcg
     7561 gtccacatag ctgaagccgc ggacattcag ccccgcgaaa tgcagcaggt cgaagcagac
     7621 cagcacggca ggcgactcgc gcactgccgt ggcgatctcg cccgcggttt tgagctgtgc
     7681 ccgattctga agcgcgttga aggagggctt tccatccgcg cccagcgcga cgatctcgcc
     7741 atcgagaatc atgtcggcac cggcctgctg cgccagatcg gcggtcagat ccggaaagac
     7801 cgccgtcaag tcgattccgc ggcgcgactg cagccgaact ctcgagccga gcacgaacgc
     7861 aagcacgcga tacccatcca gcttcggctc atacaaccaa tccacgctcg aacggggcat
     7921 cgagccggtc tcggccagca tcggactgag cttcgcaggc atgttctctt cgggtcccga
     7981 gggcgtcagg tgagccgccg ctgtccgctg caccgactgg agcgactcgg catcctccac
     8041 caccacgttc gagagcaccg agcggctccg agccagcagc tcaccgccag cgacgaaccg
     8101 atccttgtgt ttgatcagca gccactgctt gtcttgccga gtccggacca gtgcgaagga
     8161 gcctttcagc ttctccccgc gcaggaacaa actcagcttg ccctcagcga gctcacggcg
     8221 cagacgcgct tgcgcctcct cgcgatcacc gaaggagtac ttatggtctt catcgggtga
     8281 gtacacgccg cagtcccaca cgattacgtt accagcgccg tactgtttgg caggaatgac
     8341 cccttcgaac gaggcgtatt cgaacggatg gtcctcgacc tcgaccgcca tacgcttatc
     8401 gcccgcggtg agtgaaggcc ccttgggcac ggcccacgat ttcagcacgc catccagctc
     8461 cagccgaaag tcatagtgca gccgtcgggc ggcatgcttc tggacgacga aaagcagcgg
     8521 accgcgccgc ctcggcgcga cttcggcgcc aggctccgga gtgcgcgtga aggttcgctt
     8581 ggcggcgtag gggtcgagcg gccgcttcat gcgctgagca cgatggggag ccagtcggtg
     8641 agggtttgcg ccaagtcctg aacgacttgt gcgtccttac gtccgctccg ggccgggaca
     8701 tggaacgagt gatcggcgtc ctccatccac ttcagggtgg cgcgcggggc gagtcggccc
     8761 accacgcctt gcagcagact caactcagcg agctcatcgc gcgtgccctg cagaaacaac
     8821 agcggcaggt ccacttcctg caagtgctgc gcgcgctcct cggaagggcg gcccgccgga
     8881 tgcagcggaa agcccagaaa agccaggccc cgaacggccg gaagaggctg ctgggcctgc
     8941 gcctgggagg tcatccgtcc cccgaatgag cggccaccgg caatgagggg tacacctgga
     9001 cactgttgac tggcttcttc aacagcagca cgcacggtag cgtggcaaac cgcggggacg
     9061 tccgggcgcc ttgagccagc ctccatgtac ggaaactgga agcgtaccgt ggcgatgtcg
     9121 cgctccacga gagcgctggc catggccgcc atgaagggat gttgcatgcc ggcgccggcg
     9181 ccgtgcgcga aaacaaagca ggcgctcggc ttttgcggca gcatgagtag cgcggagacc
     9241 cggcgtttgg cgtccaccgc gatcgtggcg ggctgtgcgc tcggaagtga cacgccgctc
     9301 atggtacccc aaggggagcc gcacagtggc gaggggacgg tccattggcc agctggccgg
     9361 gttctgaggt actttctgcc ggaggaggga tcttgcatgt ccggagaaat tcctgaaggc
     9421 gcgtcgtgct acgagggccg ttgccactgc ggaggcatcg gctactccta ctacacgcgc
     9481 tctgcgcccg acaagtggtc cgtgagggct tgtcagtgct cgttctgtcg cgcgcacgga
     9541 gcgctttcga cctccgaccc ttcgggattc gtaatctttc acgcccgcgc cggagcgcct
     9601 cagattcggt accgtttcgg gcaacggacc gcggactttc tgatctgcgg tgattgcggt
     9661 gtgtatgtcg cagccgtgat cgacacggac cggggcgcct tcaccgtagt gaacatcaat
     9721 gcattgaatc cccgtatcga gcaccttccg gcgccgcagc ccatgtcata cgacacggag
     9781 acacccgagc agcgaatcca ccggcgcatg cagcgctgga cgccctgcaa gcgaatctga
     9841 aggttttttc atcgtcacgt gcttgcgatc cgtgatgcga tcggagcaac atgttgcccc
     9901 cagggcaact ggtgcgcggc cattcctgcc catgaccgga gaaaacgatg tgtgacgcgg
     9961 atgcaatgga tgacatggtg gagtacaaac tgcgctcggg agagctgtcg cggcgcagct
    10021 ttggtgcgct gaccctgggt gtaggccttg ccagcgtact gccccaggcc gcgggtgccg
    10081 ccgaagtgaa ggactccgag gtcaacatca agacccctga cggtacggcg gatgcgtact
    10141 tcgtgcaccc cgcaaagggc aaacatcccg gcgtcctggt ctggcccgac attttcggtc
    10201 tgcgaccggc tttccggcag atggcgaagc gcctgtccga atccggctac gctgtgctgg
    10261 tcgtcaatcc gttctaccgc accaagaagg ctccgacggc acccgagcat cccgatttca
    10321 acgatcccgc gacccgtcag gccctcatga gccttgcggg aacgctgaat ccgaccacca
    10381 ccgtcactga cgcgaaagca ttcataccct ggctgcagaa gcaatccgcc gttgaccgta
    10441 agcgcgggat gggcacgacc ggctattgca tgggcggccc gttcacattg cgtacggcag
    10501 cggagtttcc ggatcaggta cgcgccggtg caaccttcca tggcggcggc ctcgtgaccg
    10561 acaaacccga cagtccgcac ctgctcattc cgaaattgaa agcgcaatac ctgttcgcga
    10621 tcgctgagaa cgacgacaag cgccaacccg aggccaagaa cgtgttgcgc gaggctttcc
    10681 agaacgcgca tctgccggcg gaaatcgagg tgtatgcggg gactcttcac ggctggtgtc
    10741 cgcccgactc tcaggtttac aaccacgacc aggcggagaa ggcctggagc cggcagctcg
    10801 ccttgttcga tcgggcgctg agctaggcga gaaggggacg gccaagccca agacccgaaa
    10861 gcctggctcc ctcgagaagg gagtcaggcc cggctcactg cgtgagccgg ttggcctgca
    10921 gggcgggctc gcgccgagcc gataccaaca tcagggccgc ggcgtacacg acgacgatca
    10981 ccacgagcca gcgcagccag tgcatcggca gtgacttgac gacgtaagcc gcgatgagaa
    11041 cgccgggaat gccgccgagg gccaagccga gcgcagcgcg caggctgtag cggcctttct
    11101 ggatgaaccg cacagcaccc accggcatga ggaaggcaca cgagcccatc atgatcggga
    11161 aagccgtcag ggggttcatg cccagcagac tcaccaggat gagacagggc gcatacagcc
    11221 cgatgccgag catcatgagt gcgcctaaca cgaagctgac cacgatcgca aagaccagcc
    11281 gaccgtcctg caggcccaag gcatccccgc cgccaggcat caactgcaga ttctgagcga
    11341 cgaagagccc cgccgagacg agcagggcca ctcccatccc gatctggatc gcacgtcgcg
    11401 gcagtcgggc gacaacgccg ccaccgagcc aggcgcccag aatggaagct ccgatcattc
    11461 ctatgagcgt caccgggtcc acgctcaccg cggatataaa gatgagtgcc tctacgaagg
    11521 tgggcggcgc gtgtccggcg ttgagcgtgc ccgggatgtc ttcgtccgcc ggacgatgct
    11581 gaagcttgaa gacggcggtc gtgggcgcga aagagcctat cccgagcgtg tccagcacat
    11641 tcgtgacgaa acccaggagg aggtcgttcc agtgtggatg gcgcgagcct tgcgccacgt
    11701 cacgccgggt tcgctcgata gaccaccagc gccaggtgaa ccaggcggcc agcgcaagaa
    11761 gaacggacag cagggttctg gtaccgatga cgatcgctcc cggaggtcag aggggctctt
    11821 gttgcgctgc gccgggattg tagcgcagct gcccgcgagc gccgagcgcg gcgtgcggag
    11881 caaatatgcg acgcgcatca cgcgcccctc cggggcaatc cgccggctgc tcccgaatct
    11941 gagatatctg gacactacac ggcgggcgat tccatttagc tttccagagt gaacatacga
    12001 cttaaagtgg ccgccctggt cgcctccctg ttcgtagttc tcggtgctgc ggaaatcatc
    12061 gtcgatcgga ccatcctgac cccgagtttc gcggagctcg agcgggcgga tgcccgcacg
    12121 gccatgcgcc gcatcgacta cgccctcgat gcgaccctcg aaggcctcga ggtgtcggcc
    12181 acggactggg gaaattgggc cgatacgtac cggttcgtcc aggaccgcaa cgccgccttc
    12241 gtgcaggaca acctcaccag catcgctctg cgccagctca acgccaacgt gctgctgatc
    12301 gtggatcccg atggcaacat catcgaatcc aggacgcagg atctgaagtc ggacaggccc
    12361 ctgacactcg atttcgccga gcgcaacacg ctggcgactg catttccatg gcgtgcgcac
    12421 ctgagagatg ggcggccggc gcgcgggctg atgcggacca atctcggcat catgatgctg
    12481 gtttccgctc ccgttctgga cggcaacggg ggtgggccgg tgcggggcat ggtgatgctg
    12541 ggtcgactcc tgtcggaagc cgaagtcgca agaattggtg cgcaatccca gactcaacta
    12601 gcactcctgc cccgccaaca gggggccgcg cgtgagcgac tcgtcgaggc gggcacgctc
    12661 acgcaggtct atcgatcctt tgacgatctg tacggtcggc cgatcatgac gttgcgggtc
    12721 gatgtgcccc gtcggattac cgggcagggt cgcgcggcca tcgtctacgc ctcggcgtat
    12781 ctggccggcg ccgcagtcat cgtcctggtc ctgctcgtca ttgtcctcaa tcggatcgtg
    12841 ctcgagccgc tggcgcgcat cacgcgccat gcggtcgcga tcggcgagga caaggatatg
    12901 gcctcgcgac tggacttccg cagtcaggat gagatcggag tgctggctcg tgaattcgat
    12961 cgcatggtgg ggcgcgtcgg agagacccgc cgccagctgg tcgatcagtc cttccaggcc
    13021 ggattcgccg agctcgccaa gggcgtgctg cataacctgg ggaatgcgat gaccccggtc
    13081 ggtgtgcgag ttgcccggtt gcgggagcga ctgcgcgagg cgccggtagc ggatgcgcag
    13141 caggcactca tggagctggc cagcgctggc gccgccccgc aaaggcgcgc cgatctggag
    13201 gaattcgtgc gactggcctg caaggaactc gccggcagcg tgcaggccgc tcaggaggac
    13261 atcgaagtca tgagccgcca gaccgccatc gtgcagagtg cgctggcgga gcagatgcgc
    13321 tcggcacgca acgagcacgt gatcgagtcc gtgaggctgc cggagctggt gggccaatct
    13381 ctggagatag tgccggacgc ctgccgacag cgcctggtca tcaagatgga agactctctg
    13441 aagcggctcg gctcgcttcg gctcgcacgc acggtgctac gtcttgtcgt gcagaacgtc
    13501 atcatcaatg cggccgatgc ggtcaaggag tcgggaaagg acaaaggtct gctgcaggtc
    13561 gaaggcgagc tcgaacgcga ggccgatggc gataagctcc atctgctgtt ccgcgacaac
    13621 gggatcggca tcgcggcgga taacctgggc agggtattcg agaagggttt ctcgaccaag
    13681 tccagggaaa ccaaccacgg catcggactg cactggtgcg cgaatgccat tgccgcgctc
    13741 ggcgggcgga tctgggcagc cagcgagggt cctggacgag gcacctccgt gcacctcgtg
    13801 atcccgctgg atcaaccggc gactgcgact gccgtcgggg cggcgtgagg tcctcatgaa
    13861 gagccagaat cggacgccca ttcgcgtgct cgtcgccgac gatgaggcgg atgtgcgcga
    13921 tgcttacacg caaatcctcc tggagacgga tgtcagtcag gaaatcgccg gatttcgcga
    13981 gttgcgcagc aggctcttca agcgggcggc ggcggagacg cagaagtccc gatcgcaggc
    14041 gcgcggtccg acattcgatc cggtcttctg cgagcacgct gaggcggcag tagccgcggt
    14101 caaa
//
LOCUS       DASRHN010000106         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1849888, whole genome shotgun sequence.
ACCESSION   DASRHN010000106 DASRHN010000000
VERSION     DASRHN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849888"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFB37_04095"
     CDS             complement(<1..222)
                     /locus_tag="VFB37_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZO21663.1"
                     /translation="MRAMVLAAGRGERLRPITDTTPKPMVPVGGQPLIEWHLQALARA
                     GFREIVINLSWLGEQIRSALGDGTRCGVRI"
     gene            complement(219..1271)
                     /locus_tag="VFB37_04100"
     CDS             complement(219..1271)
                     /locus_tag="VFB37_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZO21664.1"
                     /translation="MHQPDTRLALLQQWLCEDLGWRPERIEPASSDASFRRYFRIFRG
                     DQTFIVMDAPPDKEDVGPYLKVSRLLESIGVHVPHVHETNIASGLLLLEDLGSVQYLQ
                     RLTAGDDPEALYEDALAALARIQTLGGKAAGELAPYDRGALARELALMPEWFCGRHLQ
                     LELTATERTVIAAAFEFLIREALAQPTVFVHRDYHSRNLMVMSERNPGVIDFQDALRG
                     PIGYDLVSLLKDCYIGWPRERVERWVADYRARLRSHGGPAEADERQFLRWFDLIGVQR
                     HLKVLGIFARLWYRDGKPGYLRDLPLTLEYVRDTCRRYCELGDLSRLLELRLAPELVR
                     ANARATQTDSRLAVSS"
     gene            1331..>2974
                     /gene="lptD"
                     /locus_tag="VFB37_04105"
     CDS             1331..>2974
                     /gene="lptD"
                     /locus_tag="VFB37_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HZO21665.1"
                     /translation="MLRWRLLHIAILSLGSLACRAWAADPPPCPTQVSPLTTGSAPAS
                     TQQPGTPAGQPADKLGLKEHKPGIEEGPIDITADHATGTAGKAKLQGNVIIRQGDREI
                     RAQQADFDRDTNAVKTDGSVEYQDPVVHVTGEGGDYSPTEGADFRSAQFDLRQRAARG
                     SADTMRLTPQGVIDLKGVKFTSCPKNDEAWRLRADSVTLDTRSRIGEAHDARVDFEGV
                     PIMYLPWLSFPLGNERKSGFLFPTVGSNSRSGAELAVPYYWNIAPNADFTFDPMIYSR
                     RGADLGGDLRFLESNDHGELTWNYLPYDGVAHASRSRVKLTNVMELPDDFRFSIDAEN
                     VSDTTYFEDFAQGPEGTSTAFVQQQAAVTYRDEHWNLDAQLEHYQTIDTTLLDPALGG
                     SPLNKPYARLPRLAVSADYGLGPAEIFHYGFQSEVVNFDRSVNIERSITCALLVNGVD
                     PCVTGWRMDLMPQASLDFEAPGYFVRPGIAWRATQYALDNTDPGEDRSPLRTLPIASF
                     DTGLLFERDAGSHDQRRVTLEPRLLYLYVPYRNQDQLPLF"
BASE COUNT          556 a         1014 c          922 g          482 t
ORIGIN      
        1 gatgcgcacg ccgcagcgcg tgccatcgcc gagcgcgctg cgaatctgtt cgccgagcca
       61 cgagaggttg atgacgatct cgcggaatcc ggcccgggcg agggcctgca ggtgccattc
      121 gatcaggggc tggccaccga ccggcaccat cggcttgggt gtggtgtcag tgatcggccg
      181 aagacgctcc ccgcggccag ccgcgagcac catcgctctc atgaggaaac agcgaggcgg
      241 gaatctgtct gagtcgctcg agcattcgcg cgtaccagct cgggagccag gcgcaactcc
      301 agcagccgcg aaagatcgcc gagctcgcaa tagcgccggc aggtatcgcg gacgtattcg
      361 agcgtgagcg gcagatcgcg cagatagccg ggttttccat cccgatacca cagccgcgca
      421 aagatgccga gtaccttgag gtgccgctgc acgccgatca gatcgaacca gcgcaggaac
      481 tgccgctcgt ccgcctccgc cgggcccccg tgcgagcgca gtcttgcacg atagtcggcc
      541 acccagcgct caacgcgctc acgcggccag ccgatgtagc aatccttcag aagcgagacc
      601 aggtcatacc cgatgggacc gcgcagcgca tcctggaaat cgatcactcc gggattgcgc
      661 tcgctcatca ccatgaggtt gcgcgaatgg tagtcgcgat gcacaaagac cgtcggctgc
      721 gcgagcgctt cgcgaatcag gaactcgaac gctgcggcaa tcacagtgcg ctccgttgct
      781 gtcagctcca actgcaggtg ccgcccacag aaccactcag gcatcaaagc gagctcgcgc
      841 gcaagcgccc cccgatcata aggggcaagc tcgcccgcag cctttccacc cagcgtctga
      901 atcctcgcca gcgctgcgag cgcatcctcg tacagagctt ccggatcatc gcctgccgtc
      961 agccgctgca gatactgcac gctgccgaga tcctccagca gcagcagacc gctggcgatg
     1021 ttcgtctcgt gcacatgcgg gacgtgcacg ccgatgctct cgagtaatcg actgaccttg
     1081 aggtacggtc ccacgtcctc cttgtcaggc ggtgcatcca tgacgatgaa ggtctgatcg
     1141 ccgcggaaga tgcggaagta gcggcgaaag ctcgcatcac tcgaagccgg ctcgatccgc
     1201 tcgggccgcc acccaaggtc ctcgcacaac cattgctgca gcaacgcgag tcgggtgtca
     1261 ggctgatgca tgaggggccg gcgttttcgc acccgcggtg caggagtggg ccagtataat
     1321 gccgcccctc atgctgcgct ggcgactcct tcacatcgca atcctgtcgc tgggcagtct
     1381 cgcctgccgc gcgtgggccg cggatcctcc gccttgtccc actcaggtgt cgccgctgac
     1441 gaccggcagt gcccccgcga gcacgcagca gcccggcacc cccgcgggac agccggcgga
     1501 caaactcggt ctgaaggagc acaagcccgg aatcgaggaa ggtccgatcg acatcaccgc
     1561 cgaccacgcg acgggtaccg caggcaaggc caaactgcaa ggaaatgtga tcatccgcca
     1621 gggggaccgc gagatccgag cgcagcaggc agacttcgac cgcgacacca acgcagtcaa
     1681 gaccgacggc agcgtcgaat atcaggatcc tgtcgtgcac gtgacgggcg aaggcggcga
     1741 ctactccccc acggagggcg ccgatttcag gtccgcccag ttcgatctgc gccagcgcgc
     1801 cgcgcgcggc tccgccgaca cgatgcggct gacgccgcag ggggtcatcg atctgaaggg
     1861 agtgaagttc acgagctgtc cgaagaacga tgaggcctgg cggctgcggg cagacagcgt
     1921 gacgctcgat acgcggagcc ggatcggcga agcgcacgat gcgcgggtcg acttcgaggg
     1981 cgtgccgatc atgtacctgc cgtggctctc cttcccgctg ggcaacgagc gcaaaagcgg
     2041 tttcctcttc ccgaccgtcg gcagcaactc gcgcagcggc gccgagctcg ccgtgccgta
     2101 ctactggaat atcgcaccga atgccgattt cactttcgat cccatgattt actcgcgccg
     2161 cggcgccgat ctcggcggtg atctgcgctt cctggaatcc aacgatcacg gcgagctgac
     2221 ctggaattat cttccttacg atggcgtcgc gcacgcgagc cgcagccggg tcaagctcac
     2281 gaacgtgatg gagctgccgg atgatttccg cttctccatc gatgcggaga acgtcagcga
     2341 cacgacatac ttcgaagact tcgcgcaggg gccggagggg accagcacgg cctttgtgca
     2401 gcagcaggcg gcggtcacgt atcgcgacga gcactggaat ctcgacgccc agctggagca
     2461 ctaccagacc atcgacacga cactgctcga tccggcgctc ggcggctctc cgctcaacaa
     2521 gccttacgcg cgtcttcctc gtctcgccgt gagcgccgac tacggcctgg gtccggccga
     2581 gatcttccac tacggctttc agtcggaagt cgtcaacttc gaccgctcgg tcaacatcga
     2641 gcgctccatc acctgcgcgc tgctggtaaa tggcgtggac ccgtgcgtga cgggctggcg
     2701 catggacctc atgccgcagg cgtcactcga cttcgaggcg cccggctact tcgtgcgccc
     2761 gggcatcgcc tggcgggcga cgcagtacgc gctcgacaac acggatccgg gcgaggaccg
     2821 ctctccgctg cgaaccctgc cgatcgcgag cttcgatacc gggctcctgt tcgagcggga
     2881 tgcaggctcg cacgatcagc gccgggtgac tctcgagccc cgcctcctgt acctgtacgt
     2941 gccttatcgc aaccaggacc agctgcctct gttc
//
LOCUS       DASRHN010000107         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_528243, whole genome shotgun sequence.
ACCESSION   DASRHN010000107 DASRHN010000000
VERSION     DASRHN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528243"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFB37_04110"
     CDS             complement(<1..433)
                     /locus_tag="VFB37_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21666.1"
                     /translation="MAELAPPLAVTSGIWRRKLLLTLLLGPALLWLLALIVLPHAQLA
                     LLSLRQRVAPHVYEPSLAQYRTFFTQPLYWEVFVRTALLSAVATALTLLVAFPIAWVI
                     TKIARGRASALLFVVCLIPFWVSETVRSLGWLILLRQSGVLP"
     gene            complement(426..1547)
                     /locus_tag="VFB37_04115"
     CDS             complement(426..1547)
                     /locus_tag="VFB37_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO21667.1"
                     /translation="MSPAAAATPDLAVRSIVKSFAAHQAVQDLSFNVERGSFFSILGP
                     SGCGKTTLLRMIAGFIEPDSGEIVIGGRNMAGVGPNRRPVNMVFQQLALFPMMSVAEN
                     VAFGLARRGMARAERTRKAEAMLERVGLAGAGPKRVDELSGGQRQRVAIARSMVLEPT
                     LLLLDEPLGALDLKLREHMKIELKQLQAAFGTTFVYITHDQSEALVLSDHVAVMNRGR
                     FEQLGPPQQLYYQPATAFVAGFVGANNRICGKLQALDGGIAQIETAEGWRVRARPSTP
                     VAPGDRVEAFVRPEAANLEREAHLLPPGQASYIGRVESLLFDGANSAVLLNEEKSRFP
                     FRVALPQTGRLADLKVGEQVHFSFDPQRAVCFREPGSHG"
     gene            complement(1544..2605)
                     /locus_tag="VFB37_04120"
     CDS             complement(1544..2605)
                     /locus_tag="VFB37_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZO21668.1"
                     /translation="MRAAAASLLALLVLAGCSRVDPAKRLRIITWADYVPADVIAQFK
                     RETGIQVELTLSNNEEMISKLHATGGAGYDLAQPSQDRIVGVQREFGLYKPIDLSQVK
                     LDQFRPEILAITRRIATLDGKLYALPYLWGADGLVVNTRRTSLSDYGDLCRPDLKGET
                     SMRLRRPTLMAFAFAAGKDPFVLYDDPRAYERLMEQVGERLSACKSIVRFFYDNKDQL
                     LNAMRSGEVVAAMMWDTGGWKLNRENPNIKFIVPRSGALGWLDTFALPYKGRNDAAAY
                     KWINFTMRPEIAARITRNVGNWTAARGTEDLVDQKMRAQFNASFPGGAFKDIHWYPAI
                     PPGLEEMEGRILDRIKAAD"
     gene            complement(2651..>3322)
                     /locus_tag="VFB37_04125"
     CDS             complement(2651..>3322)
                     /locus_tag="VFB37_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21669.1"
                     /translation="APTLMETMRFLWTQRAAVHVMMGTAVCALWGWGLMFWTPVFLQR
                     TYHMSVGQAAAVTQNMHLWGGGIATLVTGFLMARSSMIDARRIVWLLAAGIAIATVAS
                     WFVYYTRDLELAKAMLWIFIPSIYFYIGPGFGLLNNLARCRMRAVFCAMVLFLANLGN
                     LVIAPPLIGALSDWFGRTHASNADSLRLAMLCLVPTGLWAAAHLYLSARDIIHDQERA
                     IGLAS"
BASE COUNT          642 a         1057 c         1070 g          553 t
ORIGIN      
        1 cgggcagcac accggactgg cgcaacagga tcagccagcc cagtgagcgg acggtctcgc
       61 tgacccagaa gggaatcagg cacacgacga ataacagcgc gcttgcccga ccgcgcgcga
      121 tcttcgtgat gacccaggct atcggaaacg ccaccagcag cgtcaaagca gtggcgacgg
      181 cggacagcag cgccgtgcga acgaacacct cccagtacaa cggctgggtg aagaacgttc
      241 gatattgcgc caggctcggc tcgtagacgt ggggagcgac tcgctgtcgc aaagagagca
      301 gggcgagctg tgcatggggc aggacgatca acgcgagtag ccacagcagg gcgggtccga
      361 ggaggagggt gagcaggagt ttgcgccgcc aaatgcccga tgtgacggcc aggggagggg
      421 cgagctcagc catgggaccc cggctcgcgg aagcacaccg cccgctgtgg atcgaaactg
      481 aagtgcacct gctcgccgac cttcagatcg gcgagccgcc cggtctgtgg cagcgccacg
      541 cggaagggaa agcggctctt ctcttcgttg agcaacacgg cagaattcgc cccgtcgaag
      601 agcagactct cgacgcgtcc gatgtagctc gcctgccccg gtggcagcag gtgcgcttcg
      661 cgttccaggt tggccgcctc gggccgcaca aatgcctcga cccgatcccc cggagccacc
      721 ggcgtgctcg ggcgcgcccg cacccgccag ccctctgcgg tctcgatctg cgctatccct
      781 ccgtcgagtg cctgcagctt gccgcagatc cggttgttgg cgccgacgaa gccggcgacg
      841 aacgcggttg caggctggta atagagctgc tgcggggggc cgagctgctc gaagcgaccg
      901 cggttcatca cggccacgtg atccgagagc accagggcct cggattgatc gtgagtgata
      961 tagacgaacg tcgttccgaa ggctgcctgc agctgcttca gctcgatctt catgtgctcg
     1021 cgcagcttca gatcgagcgc gccgaggggc tcatccagca gcaacagggt cggttccagc
     1081 accatgctgc gagcgatggc cacacgctgg cgctggccac cggaaagctc atccacgcgc
     1141 ttcggtccag cgcccgcgag ccccactcgc tcgagcatcg cttcggcctt gcgcgtgcgc
     1201 tccgctctgg ccatgccacg tcgtgcgagg ccaaacgcga cattctccgc gacgctcatc
     1261 atgggaaaga gggccagctg ctggaacacc atgttgacgg gccggcggtt gggcccgacc
     1321 cctgccatgt tgcggccacc gatcacgatc tcgccggagt cgggctcgat gaagccggcg
     1381 atcatgcgca gcaacgtggt tttgccgcac cccgatggcc cgaggatgga aaagaagctc
     1441 ccgcgctcga cgttgaacga cagatcctga acggcctgat gagctgcaaa gctcttgacg
     1501 atgctgcgca ccgccagatc cggcgtcgct gcggcagcgg gactcaatca gccgccttga
     1561 ttcgatcgag aatgcggcct tccatctctt ccaatcctgg cgggatcgcc ggataccagt
     1621 ggatgtcctt gaacgcgccg ccgggaaagc tggcgttgaa ttgcgcccgc attttctggt
     1681 ccaccagatc ctcggttccc cgtgcagccg tccagttgcc gacattgcgc gtgatccgtg
     1741 ctgcgatctc ggggcgcatc gtgaagttga tccatttgta ggccgcggcg tcgttgcgac
     1801 ccttgtaggg cagggcgaag gtatcgagcc atcccagggc gccggagcgc ggcacgatga
     1861 acttgatgtt tggattctcg cgattcaatt tccagccgcc cgtgtcccac atcatcgccg
     1921 cgacgacctc gccggagcgc attgcgttga ggagctggtc cttgttgtca tagaagaacc
     1981 tgacgatgga cttgcaggcg gacagcctct cgccgacctg ttccatgagc ctctcgtatg
     2041 cccgtggatc atcataaagc acaaacgggt ctttacccgc tgcgaacgca aacgccatca
     2101 aggtcggacg gcgcaatctc atggaggtct cgcccttgag gtccggccgg cacagatccc
     2161 catagtccga cagtgaggta cgccgcgtgt tgacgaccag gccatccgcg ccccacagat
     2221 agggcagcgc atagagcttg ccgtcgagcg tagcgatgcg gcgtgtgatc gccaggatct
     2281 ccgggcggaa ttgatccagc ttgacctggc tcagatcgat gggcttgtag agcccgaact
     2341 cgcgctgcac accgacgatg cggtcctggg agggctgtgc caggtcgtaa cccgcgcctc
     2401 cagtagcatg gagcttggag atcatctcct cgttgttcga gagggtcagc tcgacctgga
     2461 tccccgtttc gcgtttgaac tgcgcgatga catccgctgg cacgtaatcg gcccacgtga
     2521 tgatgcgcag gcgcttggcg gggtccacac gcgagcaacc ggccagcacc agaagggcca
     2581 gcaacgacgc ggctgcggct ctcatccggt cgccttgggt agtgccgggc gaaccgtcag
     2641 cgggtagtgc tcagctcgcg agaccaatcg cccgctcctg gtcatggatg atgtcgcgtg
     2701 ctgagaggta cagatgcgcg gcggcccaga gcccggtggg caccagacac agcatcgcca
     2761 gtcgcagcga gtcggcattc gaggcatgag tgcgtccgaa ccaatcgctc agagcgccga
     2821 tgaggggcgg cgcgatgacg aggttgccca gattggcgag gaacagcacc atcgcgcaga
     2881 agacagcccg catgcggcat cgcgcgaggt tgttgagcaa tccaaagccc gggccgatgt
     2941 agaagtagat cgaggggatg aagatccaca gcatcgcctt ggccaactcg aggtcgcgcg
     3001 tgtaatagac gaaccaggaa gcgaccgtgg caatcgcgat gcctgcagcg agcagccata
     3061 cgatgcggcg cgcatcgatc atactgcttc gggccatcag gaagccggtc acgagcgtcg
     3121 cgataccgcc gccccacagg tgcatgttct gcgtcacggc ggccgcctgg cccacactca
     3181 tgtgataggt gcgttgcagg aagacgggag tccagaacat gagaccccac ccccacagcg
     3241 cgcaaaccgc ggtacccatc atcacatgta ccgccgcgcg ctgggtccac aggaagcgca
     3301 tggtctccat cagggtagga gc
//
LOCUS       DASRHN010000108         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1518596, whole genome shotgun sequence.
ACCESSION   DASRHN010000108 DASRHN010000000
VERSION     DASRHN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518596"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..161
                     /locus_tag="VFB37_04130"
     CDS             30..161
                     /locus_tag="VFB37_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZO21670.1"
                     /translation="MGTYGICEECGNPIGEERLRARPEATLCIDCQRRLDGEQRRAS"
     gene            304..1494
                     /locus_tag="VFB37_04135"
     CDS             304..1494
                     /locus_tag="VFB37_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO21671.1"
                     /translation="MLSGEFPPRSGGIADYTSRLASALEHSGCSVQVVTQDETGGQTR
                     DGPYAVRSWSVGGIRAVSGAIRRFGADVVHIQYQTAAFQMNPTINFLPLLLRLNGVRA
                     PVVTTFHDFRAPFLFRGAGAVRPLANHLLVGFSSAVLFTDPADLLRAHPRRPAAWIPI
                     GPNLWPSPGHRTPIDQARAWLGLPLGVGVVCYFGFLNHSKGVDTLIESIRLLADSGRN
                     IRLLLIGDALGSSDPSNRETLGRVNADIERLGLQDHVMRSGPLLPREVSTWLAASDCA
                     ALPFVDGASLRRGSLLACLAHAIPVVSTFPEPMPRLADRWLVAPFCNQEEYRLSGAEL
                     ALVRPGDSHSLAGTIGQLLDSPPEAHAMGLRGRALAERLGWPRIADATMSMYERALSA
                     NESL"
     gene            1481..>2346
                     /locus_tag="VFB37_04140"
     CDS             1481..>2346
                     /locus_tag="VFB37_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21672.1"
                     /translation="MSHCDHLSEDFPSVCRALPDGRSVPVVIVPTRTLAILPVLGILA
                     AAALGLWAAEAAQSALPIVVLADESALVLCVAILLRPAVGISCFVVVAMVLPFAVVPA
                     RLLVAPTFVDLTLGVTLASWIATSLLRPRPLILSATHLVLLAVCGLVLVAAILGAAFS
                     PLTSETLRLVLKLINAMLVFFSVTQLFNGDSQVRSVVRLIVGCGAVGALLALVIYYLP
                     FNAAVATLSALGDLGYPGGPEVVRFLAGTDVVRATGTAVDPNILGGTLMMSGLLGLGL
                     LVGERIRWRQVLL"
BASE COUNT          351 a          744 c          750 g          501 t
ORIGIN      
        1 aggtcgagca tgcgatggcc cgaagtcaca tgggaaccta cgggatctgt gaggagtgcg
       61 ggaatccgat tggggaagag cggctgcgag cccgcccaga ggcgactctc tgtattgatt
      121 gtcagcggcg actcgatggg gagcagcgcc gcgcctcgta gccggccaag cggcgggcgg
      181 acgtgacgct tcattcggga agtgcacgct cggatagcgc aggctcgcca gacgttccgt
      241 gcgcatcaaa gcgcaatggc tgaacctgca tttggcttag cggcggcacg acttcgcgtc
      301 tgcatgctca gcggtgaatt ccctccacgt agcggtggaa tcgcggacta cacttcgcgc
      361 ctggcatctg ctctggaaca tagcggctgt tcagtccagg tggtcacgca ggacgagact
      421 ggcggccaaa cccgggacgg gccatatgcc gtccgatcct ggagcgtggg cggaatccgc
      481 gcggtctcgg gagcgatacg tcggtttggc gccgatgtgg ttcacatcca gtatcagacc
      541 gcagcatttc agatgaaccc cacgatcaat tttctccccc tcctgctcag gttaaatggg
      601 gttcgcgcgc cggtggttac gaccttccac gatttccgtg caccgttcct gtttcgcggc
      661 gccggtgctg tgcgcccact cgccaatcac ctgcttgttg gattctcctc ggccgtgctc
      721 tttaccgacc ctgccgatct gcttcgcgcc catcccaggc ggccagcggc ctggatcccc
      781 attggtccga acctatggcc ttctccaggc caccgaaccc ctatcgatca agcgcgcgct
      841 tggctcggcc ttccactcgg agtcggggtg gtctgctact tcggattcct gaaccactcc
      901 aagggagtgg acacgctgat cgagtcgatt cgactgctgg cggattcggg ccgtaacatt
      961 cggctgctcc tcattggcga tgcgctgggc agcagcgacc cgtcgaaccg cgaaacttta
     1021 gggcgcgtga atgcagacat tgagcgattg ggtttgcaag accacgtgat gcgtagcggt
     1081 ccgctgctgc ccagagaggt gagtacctgg ctcgccgcct cagactgcgc cgccctgccc
     1141 ttcgtcgacg gcgcgtcgct ccgtcgaggt agcctcctgg cctgcctggc gcacgcaatt
     1201 ccggtcgtgt cgacgttccc agaaccgatg ccacgcctgg cggatcgctg gcttgttgcc
     1261 ccgttctgca atcaggagga gtaccgtttg tcgggcgcgg aattggcgct cgtccggccc
     1321 ggcgactcac acagcctcgc tgggacgatc ggtcagcttc tggactcgcc gcctgaagct
     1381 catgcaatgg gactgcgagg ccgtgccctc gccgagcgcc tgggctggcc acgcatcgcg
     1441 gatgcgacca tgagcatgta tgagcgcgcg ctgtccgcga atgagtcatt gtgaccatct
     1501 atcggaggat ttcccgagtg tctgccgggc actgcccgat ggccggagtg tcccggtggt
     1561 gatcgtgccc actcgcacgt tggccattct acccgtgctc gggatcctgg ctgcggcggc
     1621 tctcggtcta tgggctgcgg aggctgctca aagcgcactg cctatcgttg tgctcgccga
     1681 tgaatcggcg ctcgtgttat gcgtcgcgat tcttctgcgt cccgccgtgg ggatcagctg
     1741 cttcgtggtt gtggcgatgg tgctgccatt cgcggtcgtg ccagcgcggt tgctggtggc
     1801 gccgaccttc gtcgatctca cgctcggcgt caccctggca agctggatcg ccacttcgct
     1861 gctccggcct cgaccgctca tcctttccgc tacacacctc gtgctgctcg cagtctgcgg
     1921 cctcgtgctc gtcgcggcga ttctcggtgc tgcgttcagt ccgctgacga gcgaaacgct
     1981 gcgcctggta ctgaagctca tcaatgccat gctggttttc ttcagcgtga ctcagctctt
     2041 caatggtgac agccaggttc ggagcgtggt tcgactcatc gttgggtgcg gcgccgttgg
     2101 cgccctgctc gccctggtga tctattacct gccattcaac gcggccgttg caacactgtc
     2161 agcgctgggc gacctcggct atccaggcgg gccagaggtg gtgcgattct tggctggcac
     2221 ggatgtggtg cgcgccaccg ggaccgcggt cgacccgaac atcctgggcg gaacactcat
     2281 gatgagcggc ctgctggggc tcggcctgct ggtcggggaa cgtatccgat ggcgacaggt
     2341 tctgct
//
LOCUS       DASRHN010000109         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1684518, whole genome shotgun sequence.
ACCESSION   DASRHN010000109 DASRHN010000000
VERSION     DASRHN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684518"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..413
                     /locus_tag="VFB37_04145"
     CDS             129..413
                     /locus_tag="VFB37_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HZO21673.1"
                     /translation="MNGISDVLLALAVLSAWVGTIAFLRLDTSLDQLHTVTFVNVMTI
                     GALTLAGFASRGLTPQTLKCALLWIINLAVSALLSHATARAVHFRGGEVR"
     gene            410..655
                     /locus_tag="VFB37_04150"
     CDS             410..655
                     /locus_tag="VFB37_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase subunit MbhD domain-containing
                     protein"
                     /protein_id="HZO21674.1"
                     /translation="MKPLVITVLLLTALTGLLVVLSRDPARQVFALAANGLMLGVLFF
                     VLQAPDVAYSEIAVGTAVTPLLYLATLAALRINKKHP"
     gene            652..1428
                     /locus_tag="VFB37_04155"
     CDS             652..1428
                     /locus_tag="VFB37_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HZO21675.1"
                     /translation="MNPGARLVIFLCALVITAPAAWRVMHSMPTFGAATSGYADTVNT
                     LAPPLRHVSNMVAAVNFDIRGFDTLGEETMLLCAVVGAVMLLRGSRGEQEGDEAGLLA
                     GRADIARSDATTLACRVGSTALLLFGTYVVLHGTVTPGGGFQGGVIASSALLLLYLGE
                     GYRCWRDLIHSTVLAALEGIGALVFVAAAAWPLAQGHPALDNLLPLGKLKDLYSGGVI
                     FLLNGAVGLAVTGSFGQLILEFLEETRSPEDESEPNEADT"
     gene            1431..1775
                     /locus_tag="VFB37_04160"
     CDS             1431..1775
                     /locus_tag="VFB37_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HZO21676.1"
                     /translation="MAPWLVTIWLFGIGLYGIISSRNTVHLVGCLSVCQSGSYVLLLG
                     IGFKWQAGPPIFYDHPPGTPAVDPVMQALVLTDIVVGAAVTALLLAIAIQVHKRRGTL
                     DPQHLRPLRPDA"
     gene            1775..>2735
                     /locus_tag="VFB37_04165"
     CDS             1775..>2735
                     /locus_tag="VFB37_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZO21677.1"
                     /translation="MSASALLPLTVLLPLGVACALLVLAHWLPSQASNVLAIATAVAV
                     CILCAWLARQALEGPVLHWFGGWSPRPGISSLAARSPQSVDVPGVVIGISLRADPANC
                     VVSAFCALLFAASFVFAWGYFDTIHSHFQILMLFFLAALVGFCLTHDLFNLFVWFELM
                     SIAAFAATAYPLGKSSLEGALNFTITNALASFMMLAGVGLLYARTGTLDFTLLASQAA
                     GAASPVLTAGFGLIAMALLTKGAILPFNLWLADAHAVAPSPVSMIFSGAMVPVALFGL
                     ARLVLEVFAHCAPVSGLVHTLLPVLGSLTAAIGGVMAWAQRHLK"
BASE COUNT          390 a          888 c          851 g          606 t
ORIGIN      
        1 tccgtcgcca cactgctcgc cctcagcttc gcatttgacc agccctcatc gacggacctg
       61 gcgctcacac tggcgttcct ctgcctgcca gggagcctgc tgctcgctct ttttacggag
      121 cgctggctgt gaatggcatc agcgatgtgc ttctggctct ggcagtgcta tccgcgtggg
      181 tgggtaccat cgccttcctg cgactcgaca cctcgctcga tcagctccac acagtgacct
      241 tcgtcaacgt gatgacgatc ggcgccctga cactcgcggg gtttgcgagc cggggactta
      301 cgccacaaac attgaaatgc gcccttctgt ggatcatcaa cctggccgtg agcgcattgc
      361 tgtcgcacgc tacggcgcgg gccgttcatt tccgcggagg cgaagtccgg tgaagccgtt
      421 agtcatcacc gtactgctgc tgaccgccct gaccgggttg ctggtggtgc tgtcccggga
      481 tcctgcccgg caggtctttg cgctcgccgc caacggcctg atgctgggtg ttctgttctt
      541 cgtgttgcag gcgcccgatg tggcctattc ggagatcgcc gttggaacgg ccgtaactcc
      601 cctactctac ctggccactc tggctgcgtt gagaatcaac aagaagcacc catgaatccg
      661 ggggcgcgcc tggtcatctt tctgtgcgca ctggtgatca cggcaccggc ggcgtggcgt
      721 gtcatgcact ccatgcccac gttcggcgcg gcgacctcgg gctacgccga cactgtgaac
      781 acgctggcgc cgccactgcg gcacgtatcc aacatggtgg cagccgtcaa cttcgacatc
      841 cgcggcttcg acacccttgg ggaggagacc atgttgcttt gcgcggtggt cggggcagtc
      901 atgctgctgc gcggctcacg gggagagcag gaaggcgatg aggcgggcct gctggccgga
      961 cgtgccgaca tcgcccgctc ggatgcaacg acgctggcct gtcgtgtcgg ctctaccgcg
     1021 ctgcttctct tcggcacgta cgtggtttta cacggaaccg ttacccctgg cggcggcttc
     1081 cagggcggag tcatcgcctc ttcagcgctg ttgctgctct acctgggcga gggctaccgg
     1141 tgctggcgcg acctcattca cagcactgtg ctggcagcgc tcgaaggcat cggcgcactg
     1201 gtcttcgtgg ccgcggccgc ctggccgctg gcgcaagggc accccgctct ggacaatctt
     1261 cttccgctgg gcaagctcaa agacctctat tcgggaggag tgatcttcct gctcaatggc
     1321 gcggtcggac tggctgtcac gggcagtttc ggacagctca ttctcgagtt tctggaagag
     1381 acgcgctcgc cggaggacga atccgagccc aatgaggccg acacgtgagc atggctccct
     1441 ggctggtcac gatctggctg ttcgggatcg gcctgtacgg catcatctcc tcccgcaaca
     1501 ccgtccacct ggtcggatgt ctttcggtgt gccagtccgg cagttatgtt ctgctgctcg
     1561 ggatcggctt caagtggcag gcgggtccgc cgatttttta tgaccacccg cccggcacac
     1621 ccgccgtcga cccggtcatg caggcattgg tattgaccga catcgtggtg ggagcggcgg
     1681 tcacggcgtt gctgctcgcg atcgccatcc aggtccacaa gcgacgcggc accctggacc
     1741 cgcaacatct ccggcctctg cgtccggatg catgatgtcg gcgagcgccc tgctgccatt
     1801 gaccgtcttg ctgcctctcg gcgtcgcttg cgcactgctg gtgctggcac actggctgcc
     1861 gtcgcaagct tccaacgtgc tcgcgatcgc gacagccgtc gcggtttgca tcctgtgtgc
     1921 gtggcttgcc cgccaggctc tcgaaggacc cgttctgcac tggtttggag gctggtcccc
     1981 ccgacccggg atttcctcgc tggctgctcg gtcaccgcaa tcggtcgatg tacctggagt
     2041 ggtcatcgga atcagcctgc gggcagatcc ggcgaactgt gtcgtgtccg cgttctgcgc
     2101 gctgttgttc gccgcgtcat tcgtattcgc gtggggatac ttcgatacca tccattcgca
     2161 cttccagatc ctgatgctgt tcttcctggc ggccctggtg ggcttctgcc tgactcacga
     2221 tctgttcaat ctcttcgtct ggttcgagct catgagcatc gcggcgtttg ccgcaaccgc
     2281 ttatccgctc gggaagagct ccctcgaagg agcgctcaac ttcaccatta ccaacgccct
     2341 ggccagcttc atgatgttgg ccggcgtggg actgttgtat gcccgcaccg ggacgttgga
     2401 tttcaccctg ctcgcgtccc aggcggcagg agcagcgtcg cccgtattga cggccggatt
     2461 cggcctgatc gcgatggcct tgctgaccaa gggtgcgatt ctgcccttca atctttggct
     2521 ggcggacgcg cacgcggtgg cgccaagccc ggtctcgatg atcttttccg gcgcgatggt
     2581 cccggtagcg ctgttcgggt tggcgcggct ggtactggaa gtcttcgcgc attgcgcgcc
     2641 cgtgtcgggc ctggtacaca ctctgctgcc ggtgctgggc tcattgacgg ccgctatcgg
     2701 aggcgtcatg gcctgggcac aacgccacct gaaga
//
LOCUS       DASRHN010000110         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1354917, whole genome shotgun sequence.
ACCESSION   DASRHN010000110 DASRHN010000000
VERSION     DASRHN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354917"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFB37_04170"
     CDS             complement(<1..480)
                     /locus_tag="VFB37_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZO21678.1"
                     /translation="MKIAAIQMTSGPEVAANLEQARPLLEAAAQEGSLLAVLPENFAF
                     LGLRDIDKREIAEADGSGPIQDFLAAHARALGMWIVAGTVPLRSGTDGRVAAASLVYD
                     SGGQRAARYDKIHLFDVDIPGRAEKYRESAHVAPGSSVVVVDTPVGKLGLSVCYDVRF
                     "
     gene            complement(521..>2972)
                     /locus_tag="VFB37_04175"
     CDS             complement(521..>2972)
                     /locus_tag="VFB37_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HZO21679.1"
                     /translation="IARVASGWQLTTDAFTLEHERVRLTVAGTLRQDAAHAKPTISAR
                     GTLAGADIPLLKQLLGDSSAQAFGAVASRLTAGRIRNAEFALHGPVAELPFGGHADAF
                     TGSLSLQEAILSGGDLWPDAEAVDARVAWSGAKIQAIISGGHAGAFQLTGARAEWDAA
                     GRSATRLSGRITGRLEEAIAWMEEHPGLQSFAPQVQDIPASGAATFDFRVSVPPDAEG
                     APLPSSAAALQTSRIQARVTTSLRGARLQAVAGLPPMEALTGSFVFDGGHLQRSSLSG
                     MWFGGPVTLRLGEHMQAGVQVFAIQAQGVLSAKQLANAANAPATAQGNADWNGELEYV
                     TASTSRPSSWRMHAESNLLGVLSTLPAPFAKKPALALPAHVEITGAADSAELHASLGD
                     RLRSSLELRKHPEGWRVSRGAVRFDSTPPSLPVAPIVLVQGRLSRLDLPDYVAAWQQL
                     RWDNLPRVHVQIVAADMLLGGEVYPDVSLQADGTQAGTQLQLQSADLEGEASWPTRGA
                     SAHQAGADGSPGAAEIHLTRLTLAHGVGPGEEVAMLAALAPLTRVSVDVLLWQGRSLG
                     RLSATLATHGNSVAAEDLLLANDSHTAHGALHCQAELVRCRLSFSLASTDVATTLKDF
                     GFRSDLSAAQASLGGEVEWPLAGKQPWLARLEGTVRLRLADGLTHNPKPDDPGTPFAL
                     FSVPALLHGLDAAEAARAALPQPLRELHFARLDADFQLRGGEAQTSDLHFDGDAEILM
                     RGHTGLLARDFDQQVWILRGEERLPAAIRRFGATPRVAAAWLSLRELFEGSGQADRSG
                     AELRLQGSWDDPMVVAAD"
BASE COUNT          505 a         1053 c          960 g          454 t
ORIGIN      
        1 gaatctcacg tcgtagcaga ccgagaggcc gagtttgccg acgggcgtgt ccacgacgac
       61 gaccgaagat ccaggcgcaa cgtgcgccga ctcgcggtac ttctccgctc gacccggaat
      121 gtcgacatcg aatagatgga ttttgtcata gcgtgcggcg cgctggccgc ccgagtcata
      181 cacgagcgaa gcagcggcca cccgcccgtc cgtgccactt cgcaggggca cggtcccggc
      241 cacgatccac atgcccagtg cgcgcgcgtg cgcggcgaga aaatcctgga tggggcctga
      301 gccatcggcc tcggcgatct cccgcttgtc gatgtcacgc agtcccagaa acgcgaagtt
      361 ctccggcagc accgcaagaa ggctcccctc ctgcgcagcc gcctcgagca gaggcctcgc
      421 ctgctcgaga ttggccgcga cttccggccc cgaggtcatc tggatggctg cgatcttcat
      481 catgctcatt tcgggcggct cgctagcgac tgcaaacggc tcagtccgca gcgactacca
      541 tcgggtcatc ccaggatcct tgcaagcgca gctcggcgcc tgagcggtcc gcctgcccac
      601 tgccctcgaa cagctcgcgc agcgacagcc aggccgccgc cacgcgcggg gtcgccccaa
      661 agcgccggat ggctgccggc agccgctcct cgccccgaag aatccagacc tgctggtcga
      721 aatcgcgagc caggagaccg gtgtggccgc gcatcaggat ctcggcatcg ccatcgaagt
      781 gcagatcgga ggtctgggct tcacccccgc gcagctggaa atccgcatcg aggcgtgcga
      841 aatgcagctc gcgcaacggc tgcggaaggg ccgctcgtgc agcctccgcg gcgtccaggc
      901 catgcaggag cgcgggtacc gaaaacagag cgaacggcgt gcccggatca tccggcttgg
      961 gattgtgcgt gagcccatct gcgagccgca gccggacggt cccttcgagc cgcgcgagcc
     1021 agggctgctt gccggccagc ggccactcca cctcgccgcc gagggaggcc tgcgcggccg
     1081 acagatcaga ccggaatccg aaatccttca gcgtcgtggc cacatcggtg ctcgcgaggc
     1141 tgaagctcaa gcggcacctg accagctcgg cctggcaatg cagcgcgccg tgggcggtat
     1201 gcgaatcatt ggcgagcagc agatcctccg ctgccacgct gttgccgtgc gtggccaggg
     1261 tagcgctcag ccgacccagg gagcggcctt gccagagaag cacatccaca gacactcgcg
     1321 tgagcggagc cagtgcggcg agcatggcca cttcttcacc gggcccaacg ccgtgcgcca
     1381 gggtgagcct cgtgagatgg atctctgccg caccagggga tccatctgcg ccggcctggt
     1441 gggccgaggc accgcgggtg ggccagctcg cctcgccctc cagatcggcc gactgaagct
     1501 gtagctgggt acccgcctgc gttccgtcgg cctgcaggct cacatccgga tacacctcgc
     1561 cgcccagaag catgtctgcc gcgacgatct ggacgtggac ccgagggaga ttgtcccagc
     1621 gcagttgctg ccaggccgcc acgtagtccg gcagatccag acggctcaga cggccctgaa
     1681 cgagcacgat cggcgccacg ggcaagctcg gcggcgtact atcaaagcgc acggcgccgc
     1741 ggctcactct ccagccctcc gggtgcttac gcagctcgag agagctgcgt agccggtcgc
     1801 cgaggctggc gtgcagctcg gctgaatcgg ccgcgccggt aatctccacg tgagcaggca
     1861 gcgcgagcgc aggtttcttc gcgaacgggg cgggcagcgt gctgagcacg ccgagcaggt
     1921 tcgactccgc gtgcattcgc cagctggacg gccgcgatgt tgaggctgtc acatactcca
     1981 gctcgccgtt ccagtctgca ttgccctggg ccgtcgcggg cgcgttggcg gcgttcgcca
     2041 gctgcttcgc actcaacacg ccctgagcct gaatggcaaa cacctggaca ccggcctgca
     2101 tgtgctcgcc cagacgcaag gtgaccggac ccccgaacca catgcctgac agactcgaac
     2161 gctgcagatg tccgccatcg aacacgaacg agccggtgag ggcttccatg ggcggcaatc
     2221 ctgcgaccgc ctgcagccgg gccccgcgca gggacgtggt cacgcgcgct tgaatcctcg
     2281 atgtctgaag cgccgccgcg ctgctcggga gcggtgcgcc ttcggcatca ggtggaacgg
     2341 acacccggaa atcgaatgtc gcagcgccac tagccggaat gtcctgaacc tggggcgcaa
     2401 aggactgcag tcccggatgc tcttccatcc acgcgatcgc ctcctcgagc cgccccgtga
     2461 tgcggccgct gagccgcgtg gcgctccggc ctgctgcatc ccactcagcg cgcgcacccg
     2521 tcagctggaa cgcgcccgcg tggccgccgg aaatgattgc ctgaattttt gcaccactcc
     2581 aggcgacgcg agcgtcgacc gcctcggcat ccggccacag atcaccgccc gacaggatcg
     2641 cctcctgcaa cgacagcgac ccggtgaatg cgtcggcgtg cccgccgaag ggcagctccg
     2701 cgaccggccc gtgcagcgcg aattccgcgt tgcggatccg gcctgctgtc agtcgcgagg
     2761 cgacggctcc gaatgcctga gcgctgctgt cccccaggag ttgcttgagc agcggaatat
     2821 ccgctccggc gagcgtcccc cgcgcactga tcgttggctt ggcgtgtgcc gcatcctgtc
     2881 gcagcgtacc cgccacagtg agtcgcaccc gctcatgctc cagcgtgaat gcgtccgtgg
     2941 tgagctgcca gccactcgcc acgcgggcaa tt
//
LOCUS       DASRHN010000111         5525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1684895, whole genome shotgun sequence.
ACCESSION   DASRHN010000111 DASRHN010000000
VERSION     DASRHN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684895"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFB37_04180"
     CDS             complement(<1..332)
                     /locus_tag="VFB37_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21680.1"
                     /translation="MNWLLRRLLALWVRFKIRPDEAAAHLRERDRPVCYALERRSAAD
                     LAVLQNACVALKLARPRKRLLPQTKDLRSFFYLSRPRGFWDERIDRRPPAQLQQMIAA
                     LAHDPHLDV"
     gene            complement(333..866)
                     /locus_tag="VFB37_04185"
     CDS             complement(333..866)
                     /locus_tag="VFB37_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO21681.1"
                     /translation="METRIARHIVFSHGQESGPWGRKISALAEVARHEGYDTHSVDYR
                     GIDEPRARVAKLVEFCKELSGDLVLVGSSLGGYVAVASASLLHARGVFLMAPALYMEG
                     LPELRQGVLDCPASIVHGWMDEVIPYEHSVRFAQSYRAALHVLDSDHRLHNQIRVIQY
                     LFEYFLIALDLPAIAFQ"
     gene            958..4584
                     /locus_tag="VFB37_04190"
     CDS             958..4584
                     /locus_tag="VFB37_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO21682.1"
                     /translation="MSAAWQFWIDRGGTFTDVIGFSPTGQLHVRKVLSARSAAGEAAQ
                     GDPGMQGARDILQTESSSRISAIKVGTTVATNALLERKGEPVVLVATAGFADALRIGY
                     QNRPDIFARRIVLPDLLYATVIEARERIDTHGSVLVPLETQTLETELRRARERGLRSV
                     AIVFLHGWQHPRHEREAAAIARSLGFEEVSVSHELSPLVRYVARGDTTVLNAYLALPL
                     RSYASGLERELQRLDPSARLELMQSNGGLAAVQSFHAMSSVLSGPAGGLIGMRWIGER
                     LGLRSLIGFDMGGTSTDVSLLDGELPRRFEHIIGGVRLQSPMLDVHTIAAGGGSILHF
                     ADGRFAAGPDSAGADPGPACYGGNGPLTLTDVQVLLGRLRPDTLPAVFGQDARSPIDE
                     SVVEREFTALATRVGEQTSQPVAIETLAEAFLEVAVEAMANAIRQVSTRQGLDAGDFT
                     LFCFGGAAGQHACRVAQAAGMRRVLVHPLASVLSAFGIGVADRLAMRRASMRQRLDEA
                     GLHMAVRRLGELEDQARGELATFATSPKGIEVQHWLELRAGDSEATLSVPAGSPAEVL
                     AGFRAAHLKRFGFAAPVQSIQIEALRVEARTASVDASALRMPQQPPLPSTSRTARVWF
                     GAWVEVPLIPSIALEGQLEGPALLIEPHSTIVLERGWRARRLAGGELMLELAAASQRG
                     ASSAGGVCAPDPARIEIFNNLFMHIAEQMGEVLKATAQSVNIKERLDYSCALFDPQGG
                     LVANAPHMPVHLGSMGASVRAVIDAQGERMGPGDSWLLNSPYHGGTHLPDMTVVTPVF
                     LSRSAGKQSGTGARPDFFVASRAHHADIGGITPGSMPPFSRTIDEEGVLFECFPLVVC
                     GELQERELREALLSARYPARNPDQNTADLRAQLAANARGIAEIERAVQRHGDDVVKAY
                     MRHVQDNAAASVRNAIDRLKPGEFRYEMDSGQLIAVRIDIDRGAQRARVDFTGTSAQD
                     VHNFNAPRAVALAAVLYVFRTLIEHSIPLNEGCLEPLEVIIPRASMLDPSPPAAVAAG
                     NVETSQCIVDTLYGALGVLAASQGTMNNLTFGDDRVQYYETIAGGAGAGPGFDGCDAV
                     QTHMTNSRLTDPEILEAKFPVLLREFSIRKGSGGAGAHRGGNGTIRRMQFLAPLSGAL
                     LANHRRVAPFGLAGGRNGAHGEGRIERVSGAVEHLAGTAAFEVQPGDELIISTPGGGG
                     FGNL"
     gene            complement(4717..>5525)
                     /locus_tag="VFB37_04195"
     CDS             complement(4717..>5525)
                     /locus_tag="VFB37_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21683.1"
                     /translation="ALLLVDPPSAWISARAALEGLRNWPFRSDSAVMYFPRVQSFDRL
                     RGRAETFASCGAAAGMIARTDETCPVWATPETEEAFLRPGLKPAAAVSELDRVRLAQV
                     DVNTLVSTRSGGRQGLTPRTLGAGTRGVSDWKYLAARRLSLLVASSIEQGTRWVLLEP
                     NIPSMWERARASVDRFLGGLAQEGAFAAALPDESHFVISDDRVNREHTVAEGKFNLLY
                     GFATSKPGEFDTWLVTHQAGSSRVRPVSVNRAATSRNRVEWEIETSILRS"
BASE COUNT          943 a         1828 c         1821 g          933 t
ORIGIN      
        1 acatccagat gcgggtcgtg tgcgagcgct gcgatcatct gctgcaattg cgcaggaggt
       61 cgccgatcga tgcgctcgtc ccagaaacct cgcggccggc tcagatagaa gaaagaccgc
      121 agatccttcg tctgcggcag caggcgcttg cgcggtctcg caagcttcag cgccacgcag
      181 gcattctgca gcaccgccag gtccgcggcg ctgcgccgct cgagcgcata gcacaccggc
      241 cggtcgcgct cgcgcaggtg cgctgccgcc tcatccggcc tgatcttgaa tcgcacccag
      301 agcgcgagca gccgccgcaa cagccagttc attcactgaa acgcgatggc cggaaggtcg
      361 agcgcgatga ggaagtactc gaacagatat tgaatgacgc ggatctggtt gtgcaggcga
      421 tgatcactgt cgaggacatg cagcgctgcc cggtaggatt gcgcaaagcg cacgctgtgc
      481 tcgtaaggga tgacttcgtc catccagcca tggacgatcg aagccggaca gtcgagcacg
      541 ccctgacgca gctccggcag gccttccatg tagagcgccg gggccatcag gaacaccccc
      601 cgggcatgca gcaaagaggc ggacgccact gcgacgtagc cgccgaggct cgagcccacc
      661 agaaccagat cgcccgagag ctccttgcag aattccacga gcttggcgac tcgcgcccgc
      721 ggctcgtcga tgccgcgata gtccaccgaa tgggtgtcgt aaccctcatg gcgcgccacc
      781 tcggccagcg cggagatctt gcggccccac ggcccgctct cctggccgtg cgagaagacg
      841 atatggcggg ctatgcgcgt ttccatggga gctcatcctg catgatcctc tccactgagg
      901 gagaataatg caggtaatgc ggatccgagg acaaatgcgc ggctggactg aatctcaatg
      961 agcgccgcct ggcagttctg gatcgatcgt ggcggcacct tcacggacgt catcggtttc
     1021 tcaccgacag gtcagctgca cgtgcgcaag gtcctctctg ctcgttccgc tgcgggcgag
     1081 gccgcgcagg gggatccggg gatgcagggt gcccgggata tcctgcagac cgagtccagt
     1141 tccaggatca gcgccatcaa ggtcggcacg accgtggcga ccaatgccct gctcgagcgc
     1201 aaaggcgagc ccgtcgtact ggttgccacc gcgggattcg ccgacgcact gcgcatcggc
     1261 tatcagaacc ggcctgacat cttcgcgcgc cggatcgtct tgccggacct tctctatgcc
     1321 acggtgattg aagcgcggga gcgcatcgac acccatgggt cggtgctcgt tcctctcgag
     1381 acgcaaaccc ttgagaccga gctgcgccgc gcccgcgagc gggggctgcg ttccgtggcg
     1441 atcgtcttcc tgcatggctg gcagcatccg cgccacgagc gggaagcggc agccatcgcc
     1501 cgcagcctcg gattcgagga ggtctccgta tcgcacgagc tttcgccgct cgtgcgatac
     1561 gttgcgcggg gcgacacgac ggtattgaac gcgtacctgg cgctcccatt gcgcagctac
     1621 gcatcgggtc tcgagcgcga gctgcagagg ctcgatccgt ccgctcgact cgagctcatg
     1681 caaagcaatg ggggcctggc ggccgtgcaa tccttccacg cgatgtcgag tgtcctgtcg
     1741 ggaccggcgg ggggcctgat cggcatgcgc tggatcggag agcggctggg gctgcgttcg
     1801 ctgatcggtt tcgacatggg tggaacatcc accgacgtct cgctgctcga tggcgagttg
     1861 ccccgccgct tcgagcacat catcggtggt gtacgcctgc aaagccccat gctggacgtg
     1921 cacaccatcg ccgccggcgg cggctccatc ctgcacttcg cagacggccg cttcgcagcc
     1981 ggtcctgatt cggccggagc cgatcccggg cccgcctgct atggggggaa cggaccgctg
     2041 acactcaccg acgttcaggt cctgctgggt cggctgcgtc cggacaccct gcccgccgtg
     2101 tttggtcagg atgctcgttc cccgatcgat gagtcggtgg tcgagcgcga attcaccgcc
     2161 ttggccaccc gggtaggtga gcagacatcg cagccggtgg ctatcgagac cctcgcagag
     2221 gcctttctgg aggttgcggt ggaagcgatg gccaacgcca ttcgccaggt ttccacccgg
     2281 caggggctcg atgcgggcga cttcacgctg ttctgcttcg gcggagccgc aggccagcac
     2341 gcgtgccgtg tagcccaggc cgccggcatg aggcgtgtac tcgttcatcc gctcgccagc
     2401 gtcctgtccg cctttggaat cggcgtggcc gaccgtctgg cgatgcgccg cgccagcatg
     2461 cggcagcggc tcgatgaggc aggcttgcat atggcggtgc gtcgcctcgg cgagcttgaa
     2521 gatcaggcgc gcggcgagct cgcaacgttt gcgaccagcc cgaagggcat cgaggtgcag
     2581 cactggctgg agctgcgggc cggcgacagt gaagcgacct tgtcagtccc ggcaggttcg
     2641 cccgcggagg tgctggccgg cttccgcgcc gcacatctga agcgcttcgg cttcgcagcg
     2701 cccgtgcaga gcattcaaat cgaggcgctg cgcgtggaag cccggacagc ttcggtggat
     2761 gcgagcgcgc tgcggatgcc ccagcagcca cccctgccct ctacctctcg gaccgcgcga
     2821 gtctggtttg gcgcgtgggt ggaggtgccc ctcataccga gcatcgcgct ggaaggtcag
     2881 ctcgaaggtc cggcgttgct catcgagccg cactcgacga tcgttctcga gcgcggctgg
     2941 cgggctcgac ggctggccgg aggcgaattg atgttggagc ttgcggccgc ttcgcagcgc
     3001 ggggcgagtt ctgccggcgg tgtgtgcgcg ccggacccgg cgcgcatcga aatcttcaac
     3061 aacctgttca tgcatatcgc cgagcagatg ggcgaggtgc tcaaggccac cgcgcaatcg
     3121 gtcaacatca aggagcggct cgactactcc tgcgcgctgt tcgatccgca gggcggcctg
     3181 gtcgcgaacg cgccgcacat gccggtgcac ctcggctcaa tgggcgcgag cgtgcgtgcg
     3241 gtcatcgatg cccagggcga gcgcatgggg ccgggtgact cctggctgct caacagtccg
     3301 taccacggcg gaacacattt gccggacatg acagtcgtga cgcccgtgtt cctatcccga
     3361 tcggccggca agcaatcggg gaccggcgca aggcccgact tcttcgttgc atcgcgcgcc
     3421 catcacgccg atatcggcgg catcacgccc ggctcgatgc cgcccttcag ccgaacgatc
     3481 gatgaagaag gcgttctgtt tgaatgcttc ccgctcgtgg tctgcggcga gctgcaggaa
     3541 cgcgagctgc gcgaggctct gctctcggcg cggtatccgg cgcgcaatcc tgaccagaac
     3601 acggcggatc tgcgcgcgca actggcggcc aatgcgcgcg gcatcgccga aatcgagcgg
     3661 gcggtacagc gtcacggcga cgacgtcgtg aaggcctaca tgcgccacgt gcaggacaat
     3721 gccgctgcca gtgtgcgcaa tgcaatcgat cgactcaagc ccggcgagtt tcgctacgag
     3781 atggacagcg gtcagctcat cgcggtgcga atcgacatcg atcgtggcgc gcagcgtgcg
     3841 cgcgtggatt tcaccggtac ctcggcccag gacgtgcaca acttcaatgc accccgggca
     3901 gtagcgctgg ccgccgtgct gtatgtcttc cgcaccctga tcgagcactc gattcccctg
     3961 aacgagggat gcctggagcc ccttgaggtg atcattccgc gcgcaagcat gctcgacccg
     4021 tccccgccgg cggcagtggc ggccggcaac gtcgaaacct cgcaatgcat tgtcgacacg
     4081 ctgtacggtg cgctgggtgt gctcgcagcc tcccagggga cgatgaacaa cctcacgttc
     4141 ggcgatgacc gcgtgcagta ctacgagacc attgcgggcg gagccggtgc gggtcccggc
     4201 ttcgacggct gcgatgccgt gcagacccac atgaccaact cacgactcac cgatccggag
     4261 atcctggagg cgaagtttcc cgtcctgttg cgcgagttct ccatccggaa gggatccggc
     4321 ggcgcaggcg cccatcgtgg aggcaacggc acgatccggc ggatgcagtt cctggcgcct
     4381 ctctcggggg cgctcctcgc caatcaccgc cgcgtcgccc cattcggact ggcgggcggt
     4441 cgcaacggcg cgcacggcga gggtcgcatt gagcgcgtat cgggcgcagt cgagcatttg
     4501 gccggcaccg ccgctttcga agtgcaaccg ggcgatgaac tgatcatctc gactcccggc
     4561 ggaggtggat tcggaaacct ctaaagaagg tcccaggtgt tgcgtgtggg agagtcccct
     4621 tgcggccgag ctctcttgcc gggggcgagc ccacacgcaa gcccagcagc ccgagcctca
     4681 agcgcaagag ggcttccccc ccatctcacg cccccgttaa cttctaagta tcgaagtctc
     4741 gatctcccac tcgacgcgat tgcgagaggt tgccgcccgg ttgaccgaca ccggtcgcac
     4801 gcggctgctg cccgcctgat gggtgacgag ccaggtgtcg aactcaccgg gcttgctcgt
     4861 ggcgaaaccg tacagcagat tgaacttgcc ttcggcgacg gtgtgctcgc gattcacgcg
     4921 gtcatcgctg atgacgaaat gactctcatc cggcagcgca gccgcgaatg cgccttcctg
     4981 cgcgaggccg cccagaaaac gatccacgct cgcgcgcgcc cgctcccaca tcgagggaat
     5041 gttcggttcg agcagcaccc aacgtgtccc ctgctcgatg ctggaggcga ccaggagtga
     5101 cagccggcgc gctgcgagat atttccagtc ggaaaccccc cgagtgcccg cgcccagagt
     5161 ccgcggggtc agaccctggc gaccgccact gcgtgtcgag accagtgtgt tcacgtccac
     5221 ctgtgcaagg cgcacccgat cgagttccga gacggcagcg gccggcttca ggccgggtcg
     5281 cagaaacgct tcctcggtct ccggtgtagc ccacacggga caggtctcat cggtgcgagc
     5341 gatcatcccg gcggcggccc cgcatgaagc gaaggtctcg gcacgaccgc gcagtcgatc
     5401 gaaagactgg acgcgcggga agtacatcac cgcgctatcg ctgcggaacg gccagtttcg
     5461 cagcccctcg agcgccgcac gggcggagat ccaggcggaa ggcggatcga cgaggagcag
     5521 tgcct
//
LOCUS       DASRHN010000112         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2180115, whole genome shotgun sequence.
ACCESSION   DASRHN010000112 DASRHN010000000
VERSION     DASRHN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180115"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFB37_04200"
     CDS             complement(<1..418)
                     /locus_tag="VFB37_04200"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZO21684.1"
                     /translation="MNRAVLYASLMLLPCATVSLGIADETHPAGGAAWHSLLEERSAP
                     AWRGWKEPGLPAGWHVAGGVLSKEGPVEDLVTSRNYGNFELELEWKIGKAGNSGIFYR
                     GTREYDHIYWSAPEYQLLDDAHAEDGRSRLTAAGSAY"
     gene            complement(432..1049)
                     /locus_tag="VFB37_04205"
     CDS             complement(432..1049)
                     /locus_tag="VFB37_04205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZO21685.1"
                     /translation="MDRRTSIKWVLAASAAWPLGARHIARAHSTQAAHGYGTDPDLLP
                     KHHPGEFWPLTLSPSQRQLAAALSDLIIPADEHSPSASAVGVVDFVDEWVSAPYPANR
                     ADRPIVLKGFTWLDGESSRRFGKPFAQLTAAEQNDICGAICNETRASPALREPARFFA
                     LYRDLTAGGFYSTPAGRKDLNYIGNVALASFEGPPAELLRKLNLP"
     gene            complement(1049..>2676)
                     /locus_tag="VFB37_04210"
     CDS             complement(1049..>2676)
                     /locus_tag="VFB37_04210"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZO21686.1"
                     /translation="DMAPLGAISTPDKPFGFYDATVDGGWQVPGEPYVRTTEEAAGRF
                     DWWRARMLGGRTNHWGRISLRNGPYDFKPRTRDGLGFDWPITHEEVQPYYDKVELLIG
                     VYGSNEGLENTPNSAPGCLLPPPKPLVSDLLMAQRAKPLGIPVIPGHRAVLTRQLDFR
                     SSPPRLHPGNLTAQKIIAEDMRRRSACIWATSCGRGCAIRANYQSTTVHLPPALASGN
                     LDITTDAMVYEITVGRDGRATGVSFIDRTTGKHQHAAARAVVLAASACESVRILLNSH
                     SAQFPNGLANSSGKVGRYLMDTVGSSLQGQIPLLESLPPLNEDAADGHQVYVPWWRYR
                     EQLRGKLDFARGYHIEYRGGRRIPDFSTAARLEWLTGGSYGVKFKQDVRRYYGSFVNF
                     DGRGEMIPNEQSYCEIDPSVKDRWGIPVLRFHWQWSEHELRQAAHMQRTFADIIEAMG
                     GRPNGPVYKDGAKAIAPGGSIIHEVGGAIMGADPKSSVTDRWSQTWDVPNLFITDGAV
                     FASNADKNPTLTIMALAWRAADRMLERMRRKEL"
BASE COUNT          463 a          879 c          852 g          482 t
ORIGIN      
        1 cataggccga tccggcagca gtgagccggc tcctgccatc ctcggcatgg gcatcatcca
       61 gaagctgata ttccggagcg ctccagtaga tgtgatcgta ctcgcgcgtg ccgcgataga
      121 agatcccgct gttgccggcc ttgccgatct tccactccag ctcgagctcg aaattgccgt
      181 agttgcggct cgtgacgagg tcctccacgg gtccctcttt cgagagcacg ccgccggcga
      241 catgccagcc tgcaggcagc cccggttcct tccagccccg ccaggccggg gcactgcgct
      301 cctccagcag cgagtgccag gccgcgcctc cggcagggtg cgtctcgtcc gcgataccta
      361 gcgatacggt tgcgcaggga agcagcatca ggcttgcata aaggaccgct ctattcatct
      421 cgcacccttc gtcatggcag gttgagtttg cgcagcagct cggccggcgg accctcgaag
      481 ctggcaagcg ccacgttacc gatgtagttc aaatccttgc ggccggctgg cgtcgaatag
      541 aaaccgccag cggtcagatc gcggtagagg gcgaagaagc gcgcgggctc gcgaagcgcg
      601 gggctggcac gtgtctcatt gcagatagcg ccgcagatgt cgttttgctc ggcggcggtg
      661 agctgcgcga aaggcttgcc gaagcggcgg ctcgactcgc catcgagcca tgtgaagcct
      721 ttcaggacga tcggccggtc ggcacgattg gcaggataag gcgcgctcac ccactcatcg
      781 acgaagtcga ccacgcccac tgccgaggca ctgggcgagt gctcgtccgc tggaatgatc
      841 agatcggaca gtgcagcggc gagctgtcgc tgggagggcg agagcgtcag gggccagaac
      901 tcgccgggat gatgcttcgg cagaagatcc ggatccgtcc cgtagccgtg cgccgcctgc
      961 gtggagtggg cccgggcgat gtgtcttgcg cccagcggcc acgcggcact cgccgccaga
     1021 acccacttga tgctggtgcg tcggtccatt acagctcctt gcgccgcatg cgctcgagca
     1081 tccgatcagc cgcccgccag gccagagcca tgatcgtgag agtcgggttc ttgtcggcgt
     1141 tcgaggcaaa caccgcacca tccgtgatga agagattcgg cacgtcccag gtctgcgacc
     1201 agcgatcggt gacggaactc ttcggatctg ctcccatgat cgcaccgccg acctcatgaa
     1261 tgatcgatcc tcccggagcg atagccttgg cgccatcctt gtacaccggg ccgttcggac
     1321 gcccacccat cgcctcgatg atatctgcaa atgttctctg catatgggca gcctgacgca
     1381 gctcgtgctc ggaccattgc cagtggaaac gaaggacggg gatgccccag cgatccttca
     1441 cggaagggtc gatctcgcag taggactgct cgttcgggat catctccccg cgcccatcga
     1501 aattgacgaa cgagccgtaa taccggcgca cgtcctgctt gaatttcaca ccgtagctgc
     1561 ccccggtcag ccattcgagc cgggcggccg tcgagaagtc gggaatgcgc cgcccacccc
     1621 ggtactcgat gtgatagccg cgcgcgaagt ccagcttgcc gcgcagttgc tcccggtatc
     1681 gccaccaggg gacatacacc tgatgaccgt ccgcggcgtc ctcattgagc ggaggcaggc
     1741 tctcgaggag cggaatctgt ccctgaagac tgctgcccac cgtatccatc agatagcggc
     1801 cgaccttgcc gctcgagttc gcgagcccgt tgggaaactg cgcgctgtgg gagttcagca
     1861 ggatgcgcac cgactcgcac gcgctcgccg cgagcacgac cgcccgggct gccgcatgct
     1921 ggtgtttgcc agtggtcctg tctatgaagc tcacaccggt cgcgcgcccg tcgcgcccga
     1981 ccgtgatctc gtagaccatc gcgtccgtcg tgatgtcgag gttcccgcta gccagcgccg
     2041 gcggcaagtg gacggtggtt gattgatagt ttgcgcgaat tgcgcagcca cgcccgcacg
     2101 atgtcgccca gatacaggcc gagcgccggc gcatgtcctc cgcgatgatc ttctgagcgg
     2161 tgaggtttcc cgggtgcagc cgcggcggcg agctgcgaaa atcgagctgt cgagtcagca
     2221 ccgcgcgatg ccccgggatg accggtatgc cgaggggttt tgcccgctgt gccatcaaca
     2281 gatcgctgac gagcggcttg gggggtggca acaggcaccc gggcgccgaa ttcggcgtgt
     2341 tctccagacc ttcattgctg ccatacacgc cgatcagcag ctcgaccttg tcgtagtaag
     2401 gctgaacctc ctcgtgggtg atcggccagt cgaatcccag gccgtcccgg gtgcgcggct
     2461 tgaagtcgta cggaccgttg cgcagcgaga tccggcccca gtggttggtt cgcccgccga
     2521 gcattcgcgc acgccaccaa tcgaaccggc cagcggcctc ctcggtcgtg cgcacatacg
     2581 gctcgcccgg aacctgccag ccgccatcca ccgtggcatc gtagaagccg aagggtttgt
     2641 cgggtgtact gatcgcgccg agcggtgcca tgtccg
//
LOCUS       DASRHN010000113         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_529590, whole genome shotgun sequence.
ACCESSION   DASRHN010000113 DASRHN010000000
VERSION     DASRHN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529590"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2496)
                     /locus_tag="VFB37_04215"
     CDS             complement(<1..>2496)
                     /locus_tag="VFB37_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HZO21687.1"
                     /translation="RLFDYGYFGLTPKEAAGTSPEQRWLLECAEEALQGAGYGERWQG
                     ERIGVFCGSGVSSYFLRNLWPLVRSPESAEAMRLIAGGTSPATRVSYLLNLTGPSLTI
                     DTACSSSLVAVHHAMQALAAGECDLALAGGATVRHLASQGYLAESGGIFSADGHCRPF
                     AEQAQGTIGSGGAGFVLLRPLRRAIEERDPILAVILGSAVNNDGRQKLAYTAPGLAGQ
                     VDVIRRAHAAAGVNPADIRFIETHGTATPLGDAIEIAALAQVFADSRDGAGRCALGTL
                     KANVGHLEAAAGIAALIKSVLVLMHRTIPPCIHSFPTHPQLALERTRFFFNEQLQSWP
                     PGDAPARGGVSAFGIGGTNAHVVLEQPPPSSPDPVESAPDLMLVSARSESAFRRICDE
                     LASYLTDNPSTRLCDVAHTLRIGRTAHVYRRAAVCADLAEAASQLRHWANDAAPPAPV
                     STGSPIVFLFPGQGSQHARMAAGLYAREGVFREHLDQCAEALLRPLGLDIRSLVLGGT
                     SPDDERAIKTALARTEISQPALFAVEYALGRLWLSYGVQPSLFIGHSVGEYAAACLAG
                     VFSLDDALRILVTRGRLTQRTAPGRMLAVRLGRHEITGLLGPRVSLAAVNGPNSCVLS
                     GEASALEEVRRKLDARDITAQVVNDTHGFHSELMQEVMEEFGNFLRTVTFSPARIPLV
                     STLHGRLVAPGTLAEPAYWLSQLRNAVEFDSAVRCAFEGSSKIFLELGPGTILSALVR
                     AQRDADRHLIVNGCRSARTEAPDHSAFLAALGQLWARGAPIHWPAARFAAGARRVPLP
                     THPFERVRCWVDPPRSEPGPAAEKPPSREPPENW"
BASE COUNT          472 a          840 c          805 g          379 t
ORIGIN      
        1 ccaattctcc ggcggctcac gcgatggtgg cttctccgca gcggggccgg gctcgctcct
       61 cggtggatcc acccaacacc ggacacgctc gaacgggtga gtcggcagag ggacgcgacg
      121 cgcgcccgct gcgaatcgcg ctgccggcca gtgaatgggc gctccccgcg cccagagctg
      181 tcccagcgct gccaggaatg cggagtgatc cggagcctcg gtccgcgccg accggcaacc
      241 gttgacgatg agatgacgat cggcatcgcg ctgcgctcgc accagagcgc tcagaatcgt
      301 ccccggtccc aactcgagga aaatcttgga cgagccctcg aacgcacagc ggaccgccga
      361 atcgaactca acggcattgc gcagctggct caaccagtac gcaggctcgg caagtgtgcc
      421 aggggcgacg agccgcccgt gaagcgtgga tacgagtgga atgcgtgccg gggagaaggt
      481 aacggtccgc aggaagttgc caaattcctc catgacctcc tgcatcagct ccgagtgaaa
      541 cccatgcgtg tcgttgacga cctgagccgt tatgtcgcgg gcatccagct tccttcgaac
      601 ctcttccagc gccgatgctt ctcccgacag cacgcagctg ttcggaccgt tgacggctgc
      661 cagcgatacg cgcggaccca gaagacccgt gatctcgtga cgtcccaacc ggaccgcaag
      721 catccggccc ggcgcggtcc gttgcgtcag tcgcccgcgg gtcacgagaa tcctcaacgc
      781 atcatcgagg gagaacactc cggcgagaca ggctgccgcg tactcaccca cactatggcc
      841 aatgaacagc gagggctgca caccgtacga gagccacaag cggcccagtg cgtattcgac
      901 cgcaaagagc gcgggctgag agatttcggt tctcgccagc gccgtcttga tggcgcgctc
      961 atcgtctggc gatgtgccac cgagcacaag cgagcggata tccaacccta gcgggcgcag
     1021 gagcgcctcg gcgcactgat cgaggtgctc gcgaaagaca ccctcccggg cgtataggcc
     1081 ggccgccatt cgtgcgtgtt gcgatccctg ccccggaaac aggaatacga tgggcgaacc
     1141 ggtactgacc ggcgccggcg gtgcggcgtc atttgcccag tggcgcagct gggaggcggc
     1201 ctccgcgagg tcagcgcaca ccgccgcgcg acgatagacg tgcgcagtcc tcccgatccg
     1261 cagggtgtgt gcaacatcgc acaaccgggt agagggattg tcagtcagat agctcgccag
     1321 ctcatcgcag atcctgcgaa aagcactttc ggagcgtgcg gacacgagca tgagatccgg
     1381 ggcgctctcc accggatccg gggacgatgg tggcggttgc tcgagcacca cgtgcgcgtt
     1441 cgtgccaccg attccaaacg cgctgacacc cccacgcgcg ggcgcatcac ctggaggcca
     1501 gctctggagc tgctcgttga aaaagaagcg cgtgcgctcg agagcgagct gcgggtgagt
     1561 gggaaaggaa tggatgcacg gcgggatcgt tcgatgcatc aggaccagaa ccgacttgat
     1621 gagcgcggcg atgccggcgg ccgcttccag gtggcccaca tttgccttga gagtacccag
     1681 ggcacagcgg cctgctccgt cgcgggagtc ggcaaagacc tgggccaacg cggcgatttc
     1741 gatcgcatcg ccaaggggtg tcgccgtccc gtgtgtctcg atgaaccgga tgtcggccgg
     1801 attcacgccc gcggccgcgt gagcccgacg gatcacatcc acctgcccgg ccagacccgg
     1861 ggccgtatag gcgagcttct gcctgccgtc gttgttgaca gccgagccca gaatgaccgc
     1921 caggatcgga tcgcgctcct cgatggcccg gcgcagtgga cgcagcagca cgaacccggc
     1981 tcccccggag ccgatcgtgc cctgagcctg ctccgcgaac ggcctgcaat ggccatccgc
     2041 ggagaatatt ccgccgctct cggccaagta gccctgcgag gcgagatgcc gcacggttgc
     2101 gccacctgcc aacgcgagat cgcactctcc ggcagccagt gcctgcatgg cgtgatgcac
     2161 cgcgaccagc gacgaggagc aggcggtatc gatcgtcaga ctcgggccgg tgagattcaa
     2221 cagatacgaa acgcgggttg ccgggctcgt ccctccggcg atgaggcgca tggcctccgc
     2281 ggactccggc gaacgcacca ggggccacag attgcgcagg aaatagctgc tgacgcccga
     2341 accgcagaag actccaattc tttcgccctg ccagcgctcc ccatagcccg cgccctgcag
     2401 ggcctcctca gcgcactcca gcagccatcg ctgctcgggt gaggtgccgg ccgcctcctt
     2461 gggggtcagg ccgaagtatc cgtaatcaaa cagccg
//
LOCUS       DASRHN010000114         7726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_529804, whole genome shotgun sequence.
ACCESSION   DASRHN010000114 DASRHN010000000
VERSION     DASRHN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529804"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFB37_04220"
     CDS             <1..560
                     /locus_tag="VFB37_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HZO21688.1"
                     /translation="VPLENVSADQRRLAKTINFGLIYGMSPFGLARQLGIDRGSAQKY
                     VERYFARYPGVKRFMDETRVKARELGYVETVFGRRLYLPEIKSGNSQLRQYAERSAIN
                     APMQGTAADLIKRAMISVDAWCQRQGSIARLIMQVHDELVLEVTDASVEHVARAVQDL
                     MTGAGELRVPLRVEIGTGASWDEAH"
     gene            692..1240
                     /locus_tag="VFB37_04225"
     CDS             692..1240
                     /locus_tag="VFB37_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZO21689.1"
                     /translation="MRHLLVGSVLALAAVVAHAQAPAVYVGAGVSRATLDDIFDQAGH
                     HIDLDNTAWKAFVGVRPIRPIGVEADYMDLGSESQNFGFGSGVHADAKAFAAYAVGFL
                     PLPVPFLDVYGKVGAARWQLRGHAEPSLFALDDNGTNFAWGAGAQAHFGPVAVRLEYE
                     QFDVRNTDGVKAVTAGVSFNFL"
     gene            complement(1471..2067)
                     /gene="yihA"
                     /locus_tag="VFB37_04230"
     CDS             complement(1471..2067)
                     /gene="yihA"
                     /locus_tag="VFB37_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZO21690.1"
                     /translation="MYTTTLVGRFSQARFLISAAAPAQFPADSGAEVAFVGRSNAGKS
                     SAINALTERKSLARTSKTPGRTRLLNFFELAPLQRLVDLPGYGYASGPVAERQMWKPL
                     IEALRTRRSLRGLFMIVDARRGVMEGDEALLSWAGPSVPLHVLLSKVDKLTRTEGAQA
                     LSAARTALGDRASVQLFSAVTKTGLEEAQRLLQAWLTS"
     gene            2108..2782
                     /locus_tag="VFB37_04235"
     CDS             2108..2782
                     /locus_tag="VFB37_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZO21691.1"
                     /translation="MAKTIRAGWAALAALAATVSLAQSAAAPTSQTPFSDGTVQAGTT
                     KSAVCSSCHGPSGNSTNPEWPRLAGQSAVYIAEQLKLFKSAVRNNPVMMPMASTLNDR
                     DIDDIAVYYEAQTPQGLEGDPSYWKAGESLYRGGDSTRNIPACMACHGPDGRGNLAAG
                     YPALRAQQSVYVMKQLRDYASGARYAGSKNPATASRNGVMMLTIAKRLSPEDIRNVAS
                     YVQGLR"
     gene            2846..3712
                     /locus_tag="VFB37_04240"
     CDS             2846..3712
                     /locus_tag="VFB37_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZO21692.1"
                     /translation="MIRSITLALVLSLLVCSCSRHEATQQPAAATPAPPNAATSMQNP
                     AQDAAAAQGEAQQATAAQEKGGDEPEEDRGQARSDAGLERIAQMPADQQLPSGKWKPG
                     VNYDPLVPAQPTSVGPGKVEVTEVFWLGCPHCYALEPYVESWLKSKPDYEQFVRVPVM
                     WGPVHRAHARLYYTLMALDRPELLQQAFDTIQQKHEMLVASTDEDTLKLQQAFAKQFG
                     ISADQYANAYNSFTVNSNLQRAEQLTKRYGVTGVPLFVIDGKYTTDVGKAGGPSELIS
                     LINDLAAAEHRH"
     gene            3764..4735
                     /locus_tag="VFB37_04245"
     CDS             3764..4735
                     /locus_tag="VFB37_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZO21693.1"
                     /translation="MLSIFVPSASGLAAAQPAGGSLPEDAIWIDLREPTVDEERLVEA
                     ALGINVPTREEMQEIEASNRFYEENGSLYMTATLVTKLDTDLPVRTQVTFILTGAKLV
                     TNRYMDPLPFRRFISHAATHPLAAPCGASVMAGLLEAVVNRIADALEKIGADVDSISS
                     EVFSRSRDKRPVPRDFRSALERIGQSGELVSQASESLVSLTRLLGFAQQSTGPQIPPE
                     AHARLRILSGDVLAMSDHSSFLVDKVQFVLDATLGMVSIDQNNILKIFSVVAVFMLPP
                     SVVTGFYGTNFEHIPWLHKTWGPWAGLAMMIVSATLPYLYFKRRGWL"
     gene            complement(4814..4890)
                     /locus_tag="VFB37_04250"
     tRNA            complement(4814..4890)
                     /locus_tag="VFB37_04250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4854..4856),aa:Arg,seq:ccg)
     gene            5083..5610
                     /locus_tag="VFB37_04255"
     CDS             5083..5610
                     /locus_tag="VFB37_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZO21694.1"
                     /translation="MSKQDTHFFNVFSVVIGLLAAVAICLFVLARVVASETQDKQVIS
                     ETQYLKNVDARLQPVGHEAVAGQDNTALAIKPAAGAAQAGSAAAGPLPKSGAEVFEQV
                     CSACHGAGIAGAPKAGDKAAWGPRIAKGKSTLYDHALHGFQGSSGVMPPKGGRADLPD
                     DLIKQAVDHMVDMAK"
     gene            complement(5667..6527)
                     /locus_tag="VFB37_04260"
     CDS             complement(5667..6527)
                     /locus_tag="VFB37_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO21695.1"
                     /translation="MVGPPGAGKSMLARRLPALLPPMTQAECLEVAAIASVSGAGFRK
                     THFGRRPVRTPHHTASAVALVGGGPRARPGEISLAHHGVLFLDELLEYDRHALEALRE
                     PLDSGVVSVARAAMQAQYPAEFQLVAAMNPCPCGRLGDPAGSCICTPTEVSRYRARIS
                     APLLDRLDMHIEVPRVDLSAFEGEARSESTATVRERVAQARKIQLARQGLCNSRLSDA
                     QVESLCRLDGSARAILESAMQRFGLSARARHRILKLARTIADLGGEVAISSLHVSEAV
                     TYRALDRKIA"
     gene            6563..>7726
                     /locus_tag="VFB37_04265"
     CDS             6563..>7726
                     /locus_tag="VFB37_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZO21696.1"
                     /translation="MLKPLFPIEGLAMTPKATPAGPARAYSYLRFSRPEQAQGDSLRR
                     QTSMAAEYAARHGLNLDSELNLRDLGVSAFRGANATVGALGGFLKAITDGLVPPGSLL
                     LVEALDRVSRQSARQAVRRLEEIIEAGVDVVTLSDGKRWTQESLDGYDFLLAILLFMR
                     GHEESATKSRRLKAAWSAKRERAARLEIQTRCVPAWVRHDGKKLALIPDRAALIRRIF
                     RMFLRGVGKIGIATALNREGVEPWGCTTGRRPAAHWHTSYLLKILRNPAVVGTYIPHV
                     VEHKDGRVARVPQPPIPGYYPRVVDDETFERAQTLLTARAHTVRQTNVRNLVGGLARC
                     PKCGATMTRVTKGSKPSKAGHPKLVCVTAKAGAGCKYHAVVAPDVERALVDSAD"
BASE COUNT         1420 a         2505 c         2446 g         1355 t
ORIGIN      
        1 gcgtgccgct cgagaatgtc tcggcggacc agcgccggct ggcgaaaacg atcaacttcg
       61 gtctcatcta cggcatgtcg ccgttcggtc tggcgcgcca gctcggcatc gatcgcggct
      121 cggcgcagaa atacgtcgag cgctactttg cgcgctaccc cggagtcaag cggttcatgg
      181 acgagacacg cgtgaaggcg cgcgagctgg gatacgtcga gacggtgttc ggacggcgtt
      241 tgtatctgcc tgagatcaaa tcaggcaaca gtcagctgcg ccaatacgcc gagcgcagcg
      301 cgatcaacgc accgatgcag ggcactgccg cggatctgat caagcgcgca atgatctcgg
      361 tcgacgcctg gtgtcagcgg cagggcagca tcgcgcgcct catcatgcag gtgcacgatg
      421 agctcgtgct cgaagtcacc gatgcttccg tcgagcacgt ggcgcgtgcc gtgcaggacc
      481 tgatgacggg ggccggagag ctgcgtgttc cactgcgcgt cgagatcggg acgggcgcga
      541 gctgggacga agcgcattaa ttccatccgg aactttggcc ttcaccgttc gtctttggca
      601 gagccacaca ccgcccgcgc gtcgtgccct tgccacagcc gcacggcgca gcacacggtc
      661 gtggcgcccg agtcacactt cgagagcacc catgcgccat ctcctggttg gttccgtcct
      721 cgcactcgct gcagtcgtcg cccacgctca ggctcctgcc gtttatgtgg gagccggggt
      781 ttcccgcgct acgctcgacg atatcttcga ccaggccggc caccatatcg atctggacaa
      841 taccgcctgg aaggccttcg tgggtgttcg cccgataagg ccgatcggtg tcgaggcgga
      901 ctatatggat ctcgggagtg agagccagaa cttcggcttc ggatcgggtg tgcacgcgga
      961 cgccaaagcc tttgccgcct atgcggtggg cttcctgccc ctccccgttc ctttcctgga
     1021 cgtctacgga aaggtcggcg ccgcacgctg gcaactgcgc ggccacgcag agccgtcgct
     1081 ctttgccctc gatgacaacg ggaccaattt tgcgtggggc gccggggccc aggcgcattt
     1141 cggtccggtg gccgtgcggc tggaatacga gcaattcgat gtgcgcaaca ccgatggggt
     1201 gaaggcggtg acggcgggcg taagcttcaa ctttctgtag cgaaaagacg acatctttaa
     1261 ggccaagcca ctgatatcga tagtcttttt tcgatatatc agtcggaact tccgtaatcc
     1321 acatccgtct aatccactga gtgctagtca ctccccgctt ccctccctga gcgggtcaac
     1381 ccggttcacg agacctgtac cgggttagtc tgccccggtg acctgttctc aggtcatcgg
     1441 ggtcttttat ctccactgca tccaagccag ctaggacgtt agccaggcct gcaggagccg
     1501 ctgtgcctcc tcgaggcccg tcttcgtcac cgcggaaaac agctgcacgc tggcgcggtc
     1561 ccccagggcg gtccgcgcgg cggacagggc ctgcgctcct tcggttcggg tgagtttgtc
     1621 caccttcgag agaagcacat ggagtggcac gctcggcccg gcccagctca acagtgcttc
     1681 atccccttcc atcacaccac gccgggcgtc gacgatcatg aacagcccgc gcagcgagcg
     1741 ccgcgtgcgc agcgcctcga tcagcggttt ccacatctgg cgctcggcca ccgggccgct
     1801 cgcatagcca tatcccggca ggtcgacgag acgctgcagc ggggcgagct cgaagaaatt
     1861 cagcagccgc gtgcggccgg gtgtcttgct cgtgcgggca agtgacttgc gctcggtaag
     1921 agcgttgatg gcactcgact tcccggcatt ggagcgcccg acgaaggcca cttcggcgcc
     1981 gctatccgcc ggaaactgtg cgggcgcagc ggcgctgatc aggaatcgtg cttgtgagaa
     2041 acggcccacg agggtcgtag tgtacaattc ggcgcccttg cgacgggacg aaaccggaaa
     2101 gaaaacaatg gcgaagacga tcagagccgg ctgggcagca ctggctgcgc tggcggcgac
     2161 cgtgagcctt gcgcagagcg ctgcggcgcc cacgagtcag acgcccttct ctgacggcac
     2221 cgtgcaggcg ggtaccacca agagtgccgt ctgttcgtcc tgccatggac cgagtggcaa
     2281 cagcaccaac cccgaatggc cgcgactcgc gggtcaaagc gctgtttaca tcgccgagca
     2341 gttgaagctg ttcaagagcg ccgttcgcaa caatccggtg atgatgccga tggcctcgac
     2401 gctgaatgat cgggacatcg atgacatcgc ggtgtactac gaagcgcaga ctccgcaggg
     2461 gctggaggga gatccttcct actggaaggc tggcgagtcc ctctatcgtg gcggcgacag
     2521 cacgcggaac atccccgctt gcatggcctg tcatgggccg gatggccgcg gcaacctggc
     2581 agcaggctac ccggcacttc gcgcccagca gtctgtctac gtcatgaagc agcttcgtga
     2641 ctacgcaagt ggcgctcgct acgccggctc caaaaatccg gccacggcca gtcgcaacgg
     2701 cgtgatgatg ctgacgatcg ccaaacgact gagccccgag gacatccgca acgtcgcttc
     2761 ctacgttcag ggcctgcgct gactgaattg gggccgccct tccggaagcg ctcgaccggc
     2821 gccctcgcca ccattgagga atccaatgat ccgatcgatc actctcgccc tcgtgctgag
     2881 tctgctcgtg tgctcctgct cacggcacga agcaactcag cagcccgccg cggccacacc
     2941 cgcgcccccg aacgcggcga cttccatgca aaacccagcg caggatgcgg cagccgcgca
     3001 gggcgaagca cagcaggcca cggctgcgca ggaaaaaggg ggcgatgagc ccgaggagga
     3061 tcggggccag gcacgcagcg atgccggcct ggagcggatt gcgcagatgc ccgcagacca
     3121 gcagcttccc tcgggcaagt ggaagcccgg cgtcaactac gacccgctcg tgccggcgca
     3181 gccgaccagc gtcgggcccg gcaaggtcga ggtgaccgag gtcttctggc tcggctgtcc
     3241 gcactgctat gcattggagc cgtacgttga gagctggctg aagagcaagc ccgactacga
     3301 gcagttcgtg cgggtgccgg tgatgtgggg cccggttcat cgcgcgcacg cgcgtttgta
     3361 ctacacgctg atggctctcg accggcccga gctgctgcaa caggccttcg acaccatcca
     3421 gcagaagcac gagatgctgg tcgccagcac cgatgaggac actctgaaac tccagcaagc
     3481 cttcgccaag cagtttggca tcagtgccga ccagtacgcc aacgcctata actcgttcac
     3541 ggtgaactcg aacctgcagc gcgccgagca gctcaccaaa cgctacggtg tcaccggagt
     3601 gccgctgttc gtgatcgacg ggaagtacac gacggatgtc ggcaaggctg gcggaccgag
     3661 tgagctgatc tctctcatca acgatctggc ggcggccgaa caccgccact gatggccgcc
     3721 cgcgcggcct gcggccagtc caactgaagc gggatcgttc gccgtgttga gcatcttcgt
     3781 tcccagtgcg agcgggctgg cagccgctca gccggcgggc ggctcgctgc ccgaagatgc
     3841 gatctggata gacctgcgcg agcccacagt cgatgaagag cggctcgtgg aggcggcact
     3901 cggaatcaac gtcccgacgc gcgaagagat gcaggagatc gaggcatcga atcgcttcta
     3961 cgaggagaac ggatcgctgt acatgacggc gactctggtc accaagctgg acaccgatct
     4021 accggtgcgg actcaggtga ccttcattct cacgggcgcg aagctcgtga ccaaccgcta
     4081 catggatccc ctgccattcc gccgcttcat cagtcatgcc gccacgcacc ccctggctgc
     4141 accgtgcggg gccagcgtga tggcaggcct gctcgaggcg gtcgtgaacc ggatcgccga
     4201 tgcgctggaa aagatcggcg cggacgtgga ttcgatctcg tcggaggtct tctcgcgctc
     4261 gcgcgataaa cgtccggtcc cgcgtgactt ccgcagcgca ctcgagcgga tcgggcaaag
     4321 cggcgagctg gtctcccagg cgagcgagag cctcgtcagc ctgacgcggc tgctcggctt
     4381 cgctcagcag agtaccggtc cgcagattcc accagaggcc cacgcgcgac tgcgcatcct
     4441 gtccggcgac gtgctcgcca tgagcgatca ctcgtccttt ctcgtcgaca aggtgcagtt
     4501 cgttctcgat gccaccctgg gcatggtcag catcgatcag aacaacattc tgaagatctt
     4561 ctcggtcgtg gccgtgttca tgctgccacc ctccgtggtc accggattct acggaaccaa
     4621 cttcgagcac atcccctggc tgcacaaaac ctggggaccc tgggcgggat tggccatgat
     4681 gatcgtctcg gccaccctgc cgtacctgta tttcaagcgc cgcggttggt tgtaagcgcc
     4741 aggcttgagt gcagcgccat ccgccagaag gtggaggggc taaccgagcg gagcagcgga
     4801 aattttgcgg gattggcgcg cccgactgga ttcgaaccag cgacctgcag cttcggaggc
     4861 tgccactcta tccaactgag ctacgggcgc acgaagctga ttttacaaga gtttccaggg
     4921 ggtcgctaat gatccgggca cgtgcgcgag ccgatctgcc tgcacctgag cggcatctgc
     4981 gcgcaatgcc tcggcctgtg ccgcgctcct cagctcccgc atcgctcggc gcgttggcgt
     5041 ggtctatact gccgcgccga tttattccac acgggtctca tcgtgagcaa gcaggacacc
     5101 catttcttca acgttttcag cgtcgtcatc gggctgctgg ccgccgtcgc gatctgtttg
     5161 ttcgtgctgg cgcgagtcgt agccagcgag acccaggaca aacaggtcat cagcgaaacg
     5221 caatacctga agaacgtgga cgctcggctc cagcctgtcg ggcatgaagc ggtggccggg
     5281 caggacaata cggcgctggc gatcaagccc gcagccggcg cggcacaggc cggcagtgcc
     5341 gcagcaggtc ccctgccaaa atcgggagcc gaggtgttcg agcaggtgtg tagcgcctgt
     5401 cacggtgccg gcatcgccgg cgcgccgaaa gcgggcgaca aggccgcatg gggtccccgc
     5461 atcgccaagg gtaagagcac tctgtacgac cacgcgctgc atggcttcca gggtagctcc
     5521 ggtgtcatgc cacccaaggg cggtcgcgcc gacctgcccg atgatctcat caagcaggcg
     5581 gtcgaccaca tggtggacat ggccaagtaa gcggcgctcg ctccagaccg ccctcgacgc
     5641 cgggcatcga gactcgggac ctcgtctcaa gcgatctttc tatccagtgc gcggtacgtg
     5701 actgcttcgc tgacgtgcag gcttgagatc gcgacttcgc cgcccagatc cgcaatcgta
     5761 cgcgccagct tgagaatccg gtgtcgggct cgggcagaga gcccgaacct ctgcatggcg
     5821 ctctccagga tggcgcgagc ggacccatcg agcctgcaga ggctctcgac ctgggcatcc
     5881 gagagtctcg agttgcacag gccctgtcgt gcgagttgga tcttgcgtgc ctgagctacc
     5941 cgttctctga ccgtggcggt gctctccgat cgcgcctcgc cctcgaaggc actcaagtcc
     6001 acgcgcggca cctcgatgtg catgtccagc cggtccagca gtggagcgga gatgcgcgcg
     6061 cggtatcggg atacttccgt cggagtgcaa atacagctgc cggcgggatc tcccagtcgg
     6121 ccgcacgggc acgggttcat cgccgccacg agctgaaatt cggccggata ctgcgcctgc
     6181 atggcggctc gcgcgacgct gacgaccccc gagtccaacg gctcacgcaa ggcctcgagc
     6241 gcatgacggt cgtattccag cagctcatcg agaaacagca cgccgtgatg cgccaggctg
     6301 atctctccgg ggcgtgcgcg cggccctcct cccaccagcg cgaccgccga agcggtgtga
     6361 tgcggagtgc gcacggggcg ccggccgaaa tgagttttcc tgaagccggc accgctcacc
     6421 gaggcgatgg cggcaacttc caggcactcg gcctgcgtca tcggcggcag cagcgcgggc
     6481 aaacgccgcg ccagcatgct cttgccggca cctggcggac cgaccatcaa caacctattg
     6541 acgataggac cccggtcgag ccatgctcaa gcccttgttt ccaatcgaag gactggcgat
     6601 gacgccgaaa gcaaccccag ccgggccagc aagggcgtac tcgtatctgc gtttcagtcg
     6661 tcccgagcag gcgcagggcg attcactccg acgccagacc agtatggccg cggagtatgc
     6721 cgcacggcat ggcctgaacc tcgattctga gctgaacctg cgcgaccttg gggtcagcgc
     6781 cttccgcgga gccaatgcca cggtaggcgc cttaggaggc ttcctgaagg cgatcactga
     6841 cggcttggta ccgcctggat cgctgctcct cgtcgaggcc ctagaccgcg tatctagaca
     6901 gagcgcccgg caagccgtgc gcaggctcga agagatcatc gaggctggcg tcgacgtcgt
     6961 gactctcagc gacggcaagc gctggacgca ggagtccctc gatggctacg acttcctgct
     7021 cgccatcctt ctgttcatga gggggcatga ggagagtgcc actaagtcgc ggcgcctcaa
     7081 ggcggcgtgg tccgccaaac gggagcgtgc agcccggctc gaaatccaga cgcggtgtgt
     7141 accggcctgg gtccgtcacg atggcaagaa gctcgccctg attcccgatc gtgccgccct
     7201 cattcgccga atcttccgca tgttcctgcg tggcgtcggc aagattggaa ttgccacagc
     7261 gctcaaccgg gaaggcgttg agccgtgggg ttgcacgacg gggcggcggc cggcggcaca
     7321 ctggcacacc tcgtatctac tgaagatact ccgtaatcct gcggtcgtcg ggacctatat
     7381 cccgcatgtc gttgagcaca aagacgggcg tgtcgcgcgt gtgccgcagc caccaattcc
     7441 tggctattac ccacgggtgg tcgatgacga gactttcgag cgtgctcaga ctctgctgac
     7501 tgccagagct catactgtcc gtcaaacgaa cgttcggaac ctggtcggcg gcctcgcacg
     7561 gtgtcccaag tgcggggcga ccatgactcg ggtcacaaaa ggctcaaagc cgagtaaggc
     7621 ggggcatccg aagctcgtct gcgtcaccgc taaagccggc gccggctgca agtaccacgc
     7681 cgttgtggct ccggatgtcg agcgtgcgct cgtagacagc gcggat
//
LOCUS       DASRHN010000115         8166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1520081, whole genome shotgun sequence.
ACCESSION   DASRHN010000115 DASRHN010000000
VERSION     DASRHN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520081"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFB37_04270"
     CDS             <1..1051
                     /locus_tag="VFB37_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZO21697.1"
                     /translation="TRQFDAASGSVIVSKDSLQEWRVLAHVRAGQLETPPFAASVPFA
                     GSPFTEVFGRSPKAAYMNVQQGNWEIWPGALEYHRREGFVGVLIYPLVFGARNVGFFV
                     LSFRREAPEVQYSELLVALAQQATLAVQLTRLAYSAKEAAVLVERTRIGQEIHDGLAQ
                     AFTGILMQLGAAEEFPPCKKKNSELGGILNRIRDLARDGLSEARRSVMALRLDQTRRA
                     GLESALRQLADRSTIPGRLNCTFEGGDFATGLRPEHEHELLRIAQEAVSNAVRHAHPN
                     NVRIKMADEPAHWQLSVADDGVGMEKHLELDSGEGFGLTSMRQRAGAIGGEWQIESQP
                     GVGTRVSVRLPKRRA"
     gene            1051..1686
                     /locus_tag="VFB37_04275"
     CDS             1051..1686
                     /locus_tag="VFB37_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21698.1"
                     /translation="MVAVLKRKIRVILADDHPVVRDGLAAMVNQQPDMEVVAEAGDGE
                     EAITLYEQHRPDVMVLDLRMPKRDGVAVVQRVLELNPKACLLIMTTYDGDEDIFQCLS
                     QGAKGYLLKDAPRQEILSAIRAVSEDRPYTSSSVAAKALQRMAKPSLTQRELDVLQLV
                     AQGRSNKDIARRLSITEGTAKTHVKAILTKLDAISRTEAVAVAHKRGMIRL"
     gene            complement(1753..1986)
                     /locus_tag="VFB37_04280"
     CDS             complement(1753..1986)
                     /locus_tag="VFB37_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21699.1"
                     /translation="MLAESGKSTGAERQPPQQEERLRTWAGHGSGAVCNGCGGAITEH
                     EIEYEVEMPRGNDVATLHFHFACYRTWTGRGIR"
     gene            complement(2087..2359)
                     /locus_tag="VFB37_04285"
     CDS             complement(2087..2359)
                     /locus_tag="VFB37_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HZO21700.1"
                     /translation="MSRNVQCVLLKREAPGLDRPPYPGELGKRIYENVSREAWGHWLK
                     HQTMLINEYRLTPIEPKARKFLETEMEKYFFGAGSQAPEGFQPPKL"
     gene            complement(2623..3714)
                     /gene="mutY"
                     /locus_tag="VFB37_04290"
     CDS             complement(2623..3714)
                     /gene="mutY"
                     /locus_tag="VFB37_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZO21701.1"
                     /translation="MRSETGVAAALIEWHAGQGRHDLPWQRDRNPYRVWVSEIMLQQT
                     QVSTVIPYFERFMKRFPDVRALADAAVDDVLHLWAGLGYYARARNLHRAAVRIRDDFG
                     GVFPTTFAEVASLPGIGRSTAGAILTFACGQRHPILDGNVRRVLARYFGIQGDPSDRA
                     VTDQLWELSERVTPHEGAEVYTQAIMDLGATVCTRRKPLCDYCPLADGCFAKRTGRQE
                     ELPTARRVRAKRQREVFMLIAMRRDGSVLLERRPESGIWGGLWCLPEFDTVSAARAYA
                     GQTLRAADAEPKLLGMVEHGFTHFNLIIRPLLLQCAGQMRVMDSIPTLWYDAKRPAHV
                     GLPAPIKSLLAELASPTMFESGMANAPRS"
     gene            complement(3817..4719)
                     /locus_tag="VFB37_04295"
     CDS             complement(3817..4719)
                     /locus_tag="VFB37_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZO21702.1"
                     /translation="MRTTIWLFLGVCAAGLAHADTKTFDRTVEADPHGRVEISNIAGR
                     IEVSGWDGKEVQIHGSLGEGVDRVDVDSGHGITSIRVIVPNHTFRSASADLQVRVPRE
                     SELEVSAVSADVGETGVQGGLQLKSVSGDVKADIFKATSEIKTVSGDIVLRGLRGQTD
                     GAELHVTTISGNIRLDHGSADFEGSTVSGDLHIRVDSTHNVRVRTTSGDIGFEGKLLH
                     GGSIEAESVSGDVTLRATPENGYDYDLSSFSGDITDCMGVKAERTSKYGPGKRLNGQE
                     GSAADGARIRVKTMSGDVELCNKS"
     gene            complement(4721..5299)
                     /locus_tag="VFB37_04300"
     CDS             complement(4721..5299)
                     /locus_tag="VFB37_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21703.1"
                     /translation="MNRKEGAITSLRELPRDIPPGRDLWPAIQARLAEESRGPKAAVT
                     GLRNRQVRMRFLAAAAMVATLAVGIWIGRSGFPVVGTPARAPLTASTANPTNEPGAVQ
                     AAYMMDGRYLRDRAALVKSLEDQLQRLPPQSRAKVMTSLATIQQSKQDLEAALGRDPS
                     NALLQELLLNTYQDEMRVLTAVHEAGDSGKGI"
     gene            complement(5301..5858)
                     /locus_tag="VFB37_04305"
     CDS             complement(5301..5858)
                     /locus_tag="VFB37_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO21704.1"
                     /translation="MAQAEPQDGRIEWEGALVQRARSGDTRAFERLYREHSGKVYGLC
                     LRMTRDPAIAEDCTQETFINAWRALDRFETRSSLGTWLHRIAVNVALAKRRKATPVLS
                     SDVAEDEAIESEWTLETPLEVEEIETAIAGLPEGARDVLVLYAIYGYSHAETAEMLGV
                     AEGTCKAQLHRARGLLRERLGVEVH"
     gene            complement(5946..6671)
                     /locus_tag="VFB37_04310"
     CDS             complement(5946..6671)
                     /locus_tag="VFB37_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21705.1"
                     /translation="MKTSPARASGFSAGGAQRRVLIVDDHPIVRQGLRRMIELEPDLV
                     VCGEVQTEREARSAIRALSPDVVIVDISLGQGDGLELVRDVHAQLPDLPMLVLSMHDE
                     SIYAERLLAAGASGYIMKQAASELLLVALRQVLAGGTYLSESLARNLGRTEGAAGVAG
                     GLDPIERLSNRELQVLSLIGRGLSSREAAEGLGLSVKTVETHRQTLKRKLNLMTNAQL
                     LQYAINWYASRSQQASQSTETEA"
     gene            complement(6706..7746)
                     /locus_tag="VFB37_04315"
     CDS             complement(6706..7746)
                     /locus_tag="VFB37_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZO21706.1"
                     /translation="MSAPTERSTQQETLFTQARILETMREGVVLIDTNTHLITLTNGT
                     FDRMFGYGGHELLGQSIDPLFSVSSLQRRRFERTLRDGSEVMPVEFECSRKNGSRFVA
                     TCVVTPLRMGGSERLLAVLNDVTERKRLEREIIEIASREQQRIGSDLHDGLGQDLTGI
                     ALILRGVAAQLRKEGSAARHEVEDVIALVNNAIESTRALARGLSPVGGERGGLAAAMQ
                     NLAARASERYGVRVAFKGTFGEALRLSDSSATHVYRIVQEALTNVLRHSRATEVLISL
                     ETCGGELHLRVDDNGRGLDQPAPDSADGLGLKIMRYRAQMLGGDLVLESSADGGTSVR
                     CTCPLDKVARAS"
     gene            7891..8106
                     /locus_tag="VFB37_04320"
     CDS             7891..8106
                     /locus_tag="VFB37_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21707.1"
                     /translation="MNPWRLWQPPSETIDAIAGVLRSHWAAAKSLQPEALWSGPATWL
                     RRQANRLVDQTAPIVEDPEPPESELTI"
BASE COUNT         1431 a         2745 c         2537 g         1453 t
ORIGIN      
        1 gacacggcaa ttcgatgcgg cgtccggctc ggtcatcgtc tccaaggatt cgctgcagga
       61 gtggcgcgtc ctggcccatg ttcgcgcggg ccagctcgag acaccgccct ttgccgcgag
      121 cgtgcccttc gccggatccc cgttcaccga ggttttcggc cgcagcccga aagccgccta
      181 catgaacgtc cagcaaggca attgggagat ctggcccggg gctctggagt atcaccggcg
      241 cgagggcttc gtcggcgtcc tgatttatcc gctcgtgttc ggcgcccgca atgttggctt
      301 cttcgtcctg tccttccggc gcgaggcgcc cgaggttcag tacagtgagc tgctcgtcgc
      361 gctcgcgcag caggccaccc ttgcggtaca gctcacgcga ctcgcgtatt cggcgaaaga
      421 ggccgcggtt ctggtcgagc gcacgcgcat cggccaggag attcatgacg ggctggcgca
      481 ggccttcacc ggcattctca tgcagctcgg ggccgcggag gaatttccgc cgtgcaagaa
      541 gaagaactcc gagctcggcg gcattctcaa ccggattcgc gacctggcgc gggatgggct
      601 ctcagaggcg cgccgttcgg tcatggcact gcgcctggat cagacccggc gcgcgggcct
      661 ggagagcgca ttgcggcagc tcgccgatcg ctcaaccata cccggccggc tcaactgcac
      721 gttcgaaggc ggagatttcg ctacgggcct tcgcccggag cacgagcatg agctgctgcg
      781 catcgcgcag gaggcggtca gcaacgccgt gcgccatgct catcccaaca acgtgcggat
      841 caagatggcc gatgagcctg cccactggca gctctcggtg gccgatgacg gcgttggcat
      901 ggagaagcat ctcgagctcg actcgggaga aggattcggg ctgaccagca tgcgtcagcg
      961 cgcaggggcc atcgggggag agtggcagat cgagagtcaa ccgggcgtcg ggacgcgggt
     1021 cagcgtgcga ttgcccaagc ggagagcctg atggttgccg tgctcaagcg aaagatccgc
     1081 gtcattctcg cggatgatca tccggtggta cgcgacgggc tcgcggccat ggtcaaccaa
     1141 cagcccgaca tggaggtggt cgcggaagcc ggcgatggcg aggaagcgat caccctctac
     1201 gagcaacacc gccccgatgt catggtgctc gatctgcgca tgcccaagcg cgacggtgtc
     1261 gccgtggtgc agcgggtcct cgagctgaat cccaaggcat gtctgctgat catgaccacg
     1321 tatgacggcg atgaagatat ctttcagtgt ctgagtcagg gagccaaggg ttacctgctg
     1381 aaagatgcgc cgcggcagga gattctgtcg gcgatccgcg ccgtcagcga agatcggccc
     1441 tatacctcat catccgtagc ggccaaggcg ctgcagcgaa tggccaagcc gagcctgacc
     1501 cagcgcgagc tcgacgttct gcagctcgtc gcccaggggc gcagcaacaa ggacatcgcc
     1561 cgccggctgt cgatcacgga aggcacggcg aagactcatg tcaaggccat cctgaccaag
     1621 ctcgatgcca tcagtcgcac ggaagcggtc gccgtagccc acaagcgggg catgattcgg
     1681 ctctagggtg aggtgatccg gctgtggggc aggcgcgatc ggctgtcgcg attcgcccaa
     1741 cactgtcggc aatcagcgaa ttccccgccc ggtccacgtg cggtagcagg cgaaatgaaa
     1801 gtgtaacgtc gccacgtcgt tgccacgcgg catctcgact tcgtattcga tctcgtgctc
     1861 ggtgatggcg ccgccacagc cgttgcaaac ggccccgctt ccatgcccgg cccaggtccg
     1921 caaccgctcc tcctgctggg ggggttgtcg ctcagcgcct gttgactttc cactttctgc
     1981 gagcatcgag gtctccatgg ctgctctccc cggtacagac tgtaggcgga gattttcgtt
     2041 ccctgctcaa cgctcgtgcg cgcacaagga tgacaaacgt attgtatcaa agcttgggcg
     2101 gttgaaagcc ttccggtgct tgcgagccgg cgccgaaaaa atatttttcc atctctgttt
     2161 cgagaaattt tcgcgccttg ggctcgatgg gcgtcaggcg gtattcgttg atcagcatcg
     2221 tctggtgttt caaccagtgt ccccaggcct cgcgcgagac gttctcgtag atccgcttgc
     2281 cgagttcccc cggatacggc ggccgatcca gccccggcgc ctcgcgcttc aacagcacgc
     2341 actgcacgtt tcgcgacatg acttttcctc atgcgacgta agtagctgat gccagtatga
     2401 aggtttcatc gagaacgtga atttgcaagc caggtgaaag cttcccggcg tatacgccag
     2461 cgctgcgttg accggcgcag caagcgtctg cgcatggcga caattcccgc agcggtcacc
     2521 cgcgtgacgc atcggcatgt caggctggaa gctggattcc ccgacccacc ttccgcgcta
     2581 cgattcagca gggattcagg caactgagcg atgaatggca cctcagctgc ggggcgcatt
     2641 cgccatcccg gactcgaaca ttgtcgggct ggcaagctcg gcgagcagac tcttgatggg
     2701 ggcgggcagg cccacgtgcg ccggccgctt cgcgtcgtac cagagcgttg gaatcgaatc
     2761 catcacccgc atctgcccgg cgcattgcag cagcagcggg cgaatgatca ggttgaagtg
     2821 cgtgaagccg tgctccacca taccgagcag cttcggctcg gcatcggcgg cacgcagtgt
     2881 ttgtccggca taggccctcg ccgctgagac ggtgtcgaat tccggcaaac accacagccc
     2941 gccccagatt ccgctctccg gccggcgctc gagcaacacg ctgccgtccc tgcgcatcgc
     3001 aatcaacatg aacacttcac gctgccgctt ggcacggacc cgacgcgcag tgggcagctc
     3061 ctcctggcgg ccggttcgtt tcgcgaagca cccatccgcg agcggacagt aatcacagag
     3121 gggtttgcgg cgagtacaga ccgtcgcccc cagatccatg atcgcctggg tatagacctc
     3181 ggccccttcg tgcggcgtga cccgttcgga cagctcccac agttggtcgg tgacggcccg
     3241 gtcggacgga tctccctgga tgccaaagta gcgcgcgagt acacgccgga cattgccatc
     3301 cagaatcgga tgccgctggc cgcaggcgaa tgtcaggatc gccccggcgg tcgagcggcc
     3361 gatgccagga aggcttgcga cttcggcgaa cgttgtcgga aacacgccgc cgaaatcgtc
     3421 ccggatacgc accgccgcgc ggtgcagatt gcgggcgcgc gcgtagtagc ccaatccggc
     3481 ccacaggtgc agcacatcgt ccacagccgc atcggcgagc gctcgaacat cggggaagcg
     3541 cttcatgaag cgctcgaagt acgggataac cgtggagacc tgggtctgct gcagcatgat
     3601 ctcggagacc cagacgcggt agggattgcg gtcgcgctgc cagggtaaat cgtgccggcc
     3661 ctgtccggcg tgccactcga tcagcgcggc ggcgacgcct gtttccgatc tcactgttgc
     3721 caccccacga tgggtagcat cgtacaccgt gagcctcagc tcttgtggca gctctacatc
     3781 gccgctcaac gctcgggcgg agctccgcgc cgccgctcag ctcttgttgc agagctccac
     3841 gtcaccgctc atcgtcttga cgcggatccg cgcgccgtcc gccgcagagc cttcctggcc
     3901 gttcagccgc ttgccgggcc cgtacttgct ggtgcgctcg gccttcaccc ccatgcagtc
     3961 ggtgatatcc ccgctgaagc tgctgagatc gtagtcatag ccattctcgg gcgtggcgcg
     4021 cagcgtgaca tccccgctga ccgactcggc ctcgatcgag cctccgtgca ggagcttgcc
     4081 ctcgaagccg atgtcgcccg aggtggtacg cacgcgcacg ttgtgcgtcg aatccacccg
     4141 aatgtgcagg tcgccactga ccgtggagcc ttcgaaatcc gccgacccat gatcgaggcg
     4201 gatattgccg ctgatcgtcg tgacatgcag ctcggcgccg tctgtctggc cgcgcagccc
     4261 gcgcagcacg atgtcaccgc tcacggtctt gatctccgag gtcgccttga aaatatcggc
     4321 tttgacatcg ccgctgaccg atttgagttg cagcccgccc tgaacgccgg tctcgccgac
     4381 atccgcactg acggcggaga cttcgagctc gctctcgcgc ggtacgcgca cctgcagatc
     4441 ggcagaggcg ctccggaagg tatggttggg aacgatgacc cggatgctcg tgatcccgtg
     4501 gccgctgtcc acatcgaccc gatcgacgcc ttcgccgagg gagccgtgga tctgcacttc
     4561 cttcccatcc caaccgctga cctcgatgcg gccggcgatg ttggagatct cgaccctgcc
     4621 gtgcggatcc gcctccaccg tgcgatcgaa ggtcttcgta tcggcatgcg cgagccccgc
     4681 cgcgcagacg cccagaaaca accaaatcgt ggtgcgcatc tcaaatcccc ttgccggaat
     4741 ctccggcctc atgcacggcg gtgagcacgc gcatctcatc ctgataagtg ttgagcagca
     4801 gctcctgcag cagcgcgttg ctcggatcgc gtccgagggc tgcttccagg tcctgcttcg
     4861 attgctggat cgtcgcgaga ctcgtcatga ccttggcacg cgactgcggt ggcagcctct
     4921 ggagctggtc ctccagagat ttcaccagcg cggcccgatc ccgcagatat ctcccatcca
     4981 tcatgtaagc ggcctgcacg gcgccgggct cattggttgg attggccgtg ctcgccgtga
     5041 ggggcgctcg agccggcgtg ccgaccaccg gaaaccccga gcggccgatc cagatgccga
     5101 cagccagcgt ggccaccatg gcggccgctg cgagaaagcg catgcggacc tgtcgattgc
     5161 gcagacccgt cacggcagcc tttggcccgc gcgactcttc cgccaggcgc gcctggatcg
     5221 ccggccacag atcccgaccg ggcgggatgt cccgtggcag ctcgcgcagc gaggtaatgg
     5281 ccccctcttt cctgttcatc tcagtgaacc tccacaccca gccgctcgcg cagcaggcca
     5341 cgcgcgcgat gcagctgtgc cttgcaggtg ccttcggcga cgcccagcat ctccgcagtc
     5401 tccgcgtggg agtagccgta gatcgcatac agcacgagca catcgcgtgc cccttcgggc
     5461 aggcctgcaa tcgccgtctc gatctcctca acttccaagg gcgtctccaa agtccactcg
     5521 ctctcgatcg cctcgtcctc ggccacgtcg ctgctcagga ccggggttgc cttgcgccgc
     5581 ttggcgagtg ccacgttgac cgcaatgcgg tgcaaccagg tccccagcga gctgcgggtc
     5641 tcgaaccgat ccagtgcgcg ccacgcgttg atgaacgtct cctgtgtgca gtcctcggcg
     5701 atggcgggat ctcgcgtcat ccgcaggcac agtccgtaca ccttgccgga gtgctcgcgg
     5761 tagaggcgct cgaacgcgcg ggtgtccccg gatctggcgc gctgaaccag cgcgccctcc
     5821 cattcgatcc gtccgtcctg cggttctgcc tgtgccatca acgatctgct ttcgtccctt
     5881 agaggcgtca tcgccccgta acgtttaagc aggggcaccc ccgtcgcagg ggcccggacc
     5941 gcccgctaag cctctgtttc tgttgactgt gaggcctgct ggctgcggct ggcgtaccag
     6001 ttgatcgcgt actgcaggag ctgggcgttt gtcataaggt tgagtttacg cttcaaggtt
     6061 tgccgatggg tctccacggt cttgaccgaa aggcccaggc cctcggccgc ctcacgcgaa
     6121 gacaggccgc ggccgatgag gctcaggacc tgcagctccc gattgctgag ccgctcgatc
     6181 ggatcgagcc cgccagccac gcccgccgcg ccctcggttc ggccgagatt gcgagcgagc
     6241 gactcgctca gataagtgcc ccccgccagc acctgccgca aggccacgag cagcagctcg
     6301 gaggcggcct gcttcatgat gtagccgctt gcgccggccg cgagcagccg ctcggcgtaa
     6361 atcgattcat cgtgcatcga aagcacgagc atcggcagat cgggaagctg ggcgtgaaca
     6421 tcgcggacca gctccaggcc atcgccctgg ccgagcgaga tgtccacgat cacgacatcc
     6481 ggcgacaagg cacgaatggc cgaacgggcc tcacgctcgg tctgcacctc cccacacacc
     6541 accagatccg gctccagctc aatcatgcgg cgcaagccct gccgcacaat cggatggtca
     6601 tcgacaatca acacacgccg ctgcgcaccg cctgcggaaa acccggaagc gcgagcgggc
     6661 gaggtcttca tgagaggcga ggcaacgttt tcatgaggcg cggattcatg aggcgcgggc
     6721 caccttgtcc agagggcacg tgcagcgcac cgaggtcccg ccatcggcgc tggactcgag
     6781 caccagatcg ccgccgagca tctgcgcccg atagcgcatg atcttgaggc ccaggccatc
     6841 ggcgctgtcg ggtgccggct gatcgaggcc gcgcccgttg tcatccacgc gcagatgcag
     6901 ctcgccgccg caggtttcga ggctgatgag cacctcggtc gcgcgactgt gacgcagcac
     6961 gttcgtgagt gcttcctgca cgatccgata aacgtgcgtc gccgacgaat cactcagccg
     7021 caaggcctcg ccaaacgtcc ccttgaacgc cacccgaacc ccataccgct cactggcacg
     7081 cgccgccaga ttctgcatgg ccgccgcgag accgccccgt tcaccgccca ccggtgacag
     7141 gccacgagcc agagcccgag tgctctcgat cgcgttgttg acgagggcga tgacatcctc
     7201 gacttcgtgc cgcgcggcgg agccttcctt acgcagttgc gcagcgactc cccgcaggat
     7261 cagcgcgatg ccggtgagat cctggccgag cccatcgtgc agatcgctgc cgatgcgctg
     7321 ctgctcgcgg ctggcgatct cgatgatctc gcgctcgagc cgcttgcgct cggtgacatc
     7381 gttgagcaca gccagcagcc gctcagatcc ccccatgcgc agcggcgtca ccacgcaggt
     7441 ggccacgaat cgcgagccat ttttgcgcga gcactcgaac tcgaccggca tcacctccga
     7501 gccatcgcgc aatgtgcgct cgaagcgccg gcgttgcaga gatgacacac tgaagagcgg
     7561 atcgatggac tggccgagca gctcgtgtcc gccgtacccg aacatccggt cgaacgtgcc
     7621 attggtgagc gtgatcagat gggtattggt atcgatcagg acgacacctt cgcgcatcgt
     7681 ctcgaggatg cgcgcctgcg tgaacagcgt ttcctgctgg gtcgaccgtt cagtcggagc
     7741 actcatcgga tcctgcgctt gcattcgcgg gctgcgacgc ccgtcacgtc gccgtggaga
     7801 gacagtttcc aacggaaccc cgtgcggagt aaaacgctct tcctcttaca catgacataa
     7861 gaagggagcg ttcctacagt gccgacgaag atgaacccgt ggcggctgtg gcagccgccg
     7921 agcgagacga tcgatgccat cgcgggcgta ctgcgctcgc actgggctgc ggccaagtcg
     7981 ctgcagcccg aggcactctg gagtggaccc gcgacctggc tgcgccggca ggcgaaccgc
     8041 ctcgtcgacc agacggctcc gatcgtcgag gatcccgaac cgccggagag cgagctcacc
     8101 atctgaccag cggcggcagg ctcatgaggt aagcctccgg atccgcatcc gtactgaaac
     8161 cgaact
//
LOCUS       DASRHN010000116        14124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1356614, whole genome shotgun sequence.
ACCESSION   DASRHN010000116 DASRHN010000000
VERSION     DASRHN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356614"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFB37_04325"
     CDS             complement(<1..192)
                     /locus_tag="VFB37_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZO21708.1"
                     /translation="MRAARIRRIYEPPSPQDGARVLVDRLWPRGVSKKSAALTLWLKE
                     VAPSPALRQWFGHEPQRWRE"
     gene            355..1440
                     /locus_tag="VFB37_04330"
     CDS             355..1440
                     /locus_tag="VFB37_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZO21709.1"
                     /translation="MSDPIPDSRAPRRRSASSTVGRGKAIQHDVLPQQARHAAAGMEV
                     SFGDSALLCAGWPAPVCIVSDGGYGVLGFEGDTSDHLDLPQWYEAHVAAWALRATPQT
                     TLWFWNSEIGWAAVHPLLERYGWRYVSANLWSKGRAHIAGNVNTGRIRRFPVVTEICV
                     QYVFEAKVDGLALREWLIREWKRTGLPLREANSACGVADAAVRKYLNPGHLWYFPPPQ
                     MFARLQAYANERGAVSGRPYFSLDGRTPADEEQWARMRAKFRCPFGVTNLWERGALRG
                     EERQVVPGGRAVHLNQKPLDLMSRIITACTDPGDVVWEPFGGLFSATIAAARAGRRAF
                     SAEIDPTYFHFGLERIRAEPGCRTSTG"
     gene            1507..3015
                     /locus_tag="VFB37_04335"
     CDS             1507..3015
                     /locus_tag="VFB37_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO21710.1"
                     /translation="MPRLDTMLSAATLIALSGTSGAPAQNARLEAAVLPQPAAPAAAV
                     LPYVRVSAKHIILNHVRVIDGMGAAEAEDRSIEIADGRIRAVGAAVDSPPADATVLEL
                     RGHTVIPGLVGMHDHMYYIARPNMDADGHSDPPLVVPQMSFSAPRLYLANGVTTLRTT
                     GSVEPYADLNLKHLIDAGALPGPHMDVTGPYLEGSHSPFIEMHQLKDADDARRTVAFW
                     ADQGVTSFKAYMNITRAELKAAIDEAHRRGLKLTGHLCAVTYPEAAALGIDDLEHGFF
                     VNTQLDPGKTPDVCPQSGGVPTLRAMDPEGAPAAELIRTLIAHHVAVTSTLPVFEQGV
                     PDHAPLWPRAMEVMTPEAREAYLYLRNRTNTLPADQAGERRLTYQHALGLERKFVAAG
                     GLLLGGPDPTGNGGVIPGFGDLREIELLVEAGFTAAEAVRIATFNGATYLGLAERIGS
                     VQPGRDADLVVLNGDPGKTITDVENVELVFKDGVGYDSAALLKSVRGRYGEY"
     gene            complement(3100..3570)
                     /locus_tag="VFB37_04340"
     CDS             complement(3100..3570)
                     /locus_tag="VFB37_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO21711.1"
                     /translation="MSTPSTQPIPPGMHTVTPHLICDGASEAIEFYRKAFGATETSRL
                     PGPGGKLMHASIRIGDSTVMLVDEMPQHGALGPKALKGSPVTVHLYVEDADAFAARAA
                     AAGARVTMPVTQMFWGDRYGQIEDPFGHRWSIATHVKDVAPGDMRKAMEQFNCQ"
     gene            3723..4355
                     /locus_tag="VFB37_04345"
     CDS             3723..4355
                     /locus_tag="VFB37_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21712.1"
                     /translation="MRHRRSLSFSALLLLGALAGGCDRLGIGAHGAPVADGAPTEQEL
                     QKIRYMSSAPSGPKGRTLYTHFEEAKTCGDFELAMRWNRPPNIESGPFNKKMVYVTAS
                     IPADLPKNSEVFIRGKIEQGETLPSGAARWYVTLQDGTRVQAIETADFYEKEEQAPQD
                     GGKASALVEPTRAHRIFCGQGVYEGLIGKDPDGDKKIPLLSMLFAMDRTK"
     gene            4491..5138
                     /locus_tag="VFB37_04350"
     CDS             4491..5138
                     /locus_tag="VFB37_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO21713.1"
                     /translation="MTHKSSTLGGLIRLLRRQKGWTLRQMSEKVGIPLSTLAKVEADK
                     LSLTYDKLQQFTSRLGLTMAEFLAHDTTAAQSPPRVVTARRSLTHSGNSIPISTPNYD
                     YEYLCADLREKRMVPIIARIRSHSIEEFGETVRHQGEEFIFVLEGSIEVHLQFYTSVT
                     LTAGQGIYLDSTMGHAYVAKDCESALVLAVCSSEDPVNLASELMSLADSETEPAI"
     gene            complement(5141..6271)
                     /locus_tag="VFB37_04355"
     CDS             complement(5141..6271)
                     /locus_tag="VFB37_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21714.1"
                     /translation="MSTAQQSAQSSWLQRYILPGLAFKAVVIGGGYATGRELAEFFLP
                     SGPRGGLLAIVVATIVWSVVCALTFSFSYSYRLLDYRAFFGKLLGRGWILFELTYLLL
                     VLLILSVFGATIGAVGHALFGWPSLAGTLLLLVSIAAVVAVGARAVEPVFKYVSVLLY
                     ATYAVFLALSLSKFGTRILSNLELSHVGAGWLSGGVTYAGYNSVAAVVILPVLRHLRS
                     HREAVIAGVLAGPLAMLPGLFFFLSMIAWYPEVGGSTLPSDFLLLRIGIPLVRLLFQC
                     MIFFALLESGCGIVHAIMERVRRAMRARTGHDPDLPVRIAVTLAALTVSVFAAQGIGL
                     VELIAKGYRFISWVLIVIFILPIILVSILKPRYLRHSDAAGS"
     gene            6385..8508
                     /locus_tag="VFB37_04360"
     CDS             6385..8508
                     /locus_tag="VFB37_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO21715.1"
                     /translation="MNQQSLSNRQLACAALSCGALLQSASVASHQAAFTLEQVMAAPY
                     PSELVGSSTGNAVAWVYETQGRRNIWVADAAHGMQGRSITNFTADDGFNIGELAWSHD
                     GQWIAYTRGGSLEDERPANVNSSPEGPTPREVWIVSTAGGEPRKLGVGHSAAFSPDGA
                     RVVFVDKDKLWSVAPGAREAAQTLIVDSGRVGSVTFSSDGRRLAFVSSRGSHSIVGVY
                     EFGSRHIVWLSASLDQDASPVFAPGGTQLAFIRVPSEKSPEFVAHASGTPWSIWVADT
                     LSGQGHRVWRADAGMGSVFQPSLSEQNLYWTRRDELIFPWEKTGWLQLYAVPVQGGPV
                     RTITTGAFEVAHLALSPDQTHLAFSSNQDDQDRLHVWTVDADSGTPVRAASDNSIEDK
                     PQVSAEGSVFALQSSANMPLHPVMLVHGHWQSLIEPPVPPMFPTDKLVRPAAVTFAAK
                     DGQQAHAQLFLPQERGQSRHPAILFFHGGPQRQMLLGFHPMDAYNWMYALNQYFVSEG
                     YIVLSVNYRGGIGYGHDYREAKDFGPAGGSELNDLLGAIAYLQARQDVDAHRLGIWGG
                     SYGGLMTALGLARASDSLAAGVDYAGLYNWASFLASVGAPIASPEAVQRAIQSSPVAT
                     IEQWKSPVLIVQADDDRNVPSQQASELIADLRSHHIEHDELMMPNEVHDLTRFASWMT
                     LFHATDDYFARHLGTKGLESGSETR"
     gene            8765..8983
                     /locus_tag="VFB37_04365"
     CDS             8765..8983
                     /locus_tag="VFB37_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21716.1"
                     /translation="MSVRNHIKLVWLVLTNRMHVEIRKHPRQDGVPHETDSTDMLKHA
                     QEASPLSGEGSDYVAPNEDSARSRSASR"
     gene            9151..9714
                     /locus_tag="VFB37_04370"
     CDS             9151..9714
                     /locus_tag="VFB37_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21717.1"
                     /translation="MVTDIIPRLHQALPQAKAWIERFLAAHESTAQPMSSVRSDRLAA
                     CFPADFLARSKIVAVDRVVFPPVESFGLFEFAALHQMEFEGITFKDTIFVRRDRKYEG
                     LYFHELVHVAQWARLGVDDFLLAYAAGLAQQSYRETPLENMAYDLQAKFEAGTLPRDL
                     IQQIGHLTDKVWADVRQFLNQADGRAP"
     gene            complement(9739..10116)
                     /locus_tag="VFB37_04375"
     CDS             complement(9739..10116)
                     /locus_tag="VFB37_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21718.1"
                     /translation="MKHALKIARALTDTGDMIRRRIPHSARFEAYRDFHADLGRALDA
                     ERQSWMGQSIECLKDRNEISRLTFDLSRTTTRAELAWTLRRVFGRHSPFLVERVASAL
                     DTFRSATGAELISGRNPLGHRGR"
     gene            10228..11460
                     /locus_tag="VFB37_04380"
     CDS             10228..11460
                     /locus_tag="VFB37_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZO21719.1"
                     /translation="MSAARPPEPPDPPRSVFLSYASEDRQAAQLIRDALPTYGLEVWY
                     DESDLQGGDAWDQKIRRQIRECDFFMPVISARTEARHEGYFRREWRLAVERTLDMADD
                     HTFLLPVVIDETPQTGARVPEKFLAVQWTRVPGGQPSAALEALCRRLASGLTITPESP
                     RKAPDAFSGTRVAPAGRYPEFPREEPGQKVRFWALAAAWPFQSAWVFFNRFPKWIRIC
                     IYIWLFVLVLSKGCTSSDDDRPEKLTPAQTKKLKQISDSYQGNADKADVAKLGAQIAR
                     EFSRAAAGNAAVASPLLAIPFSARAGDAVGRRVADSTFAQVYGRLAISRGNGVSLTGE
                     PLPDQSAAIELGRAQHAKYVLFGTVEGQPGAQELAVKMLAVTDGTVLWSKSYPAKGAD
                     PATIAAEVNAKVLSLDED"
     gene            11636..13264
                     /locus_tag="VFB37_04385"
     CDS             11636..13264
                     /locus_tag="VFB37_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO21720.1"
                     /translation="MKNWILAMAILLAAAPAAADQPTYDLVIRNARVLDGEGNPEVHA
                     DIAISGGRFVRIGRVTGHGRREIDVQNDYASPGWIDMMDQSGEVLIRNGLAENKLREG
                     VTTAIAGEGGAPVPSAKLTDYFHGLAQKGISLNFGTYYGAAQARVEVMGDVAGEPTPQ
                     QMDAMKAHVDEAMRAGAMGIATALIYPPDSFQSTSDLIQLARVAAQYDGMYASHMRDE
                     SANLLKAIRESIEIGEQAGIPVEIFHFKVAYAPGWGKLVNEAGQLIRDARARGVNIAS
                     DMYVYTAGGTGLDITVPNWVWEQGEKKGLERLKERHIRERLKREVAAGSQPGWSNLIE
                     ASGGWDHVVLANAFNPKYDAYRYKSLAYIGQQLGKDPADVAWDIVLAAQPNRAMALYF
                     MMSEQDIETALRWPWMSIGSDAGANEKEGQIDAIGLPHPRAYGNFPRVIAEYVKKRGV
                     LTLEDAIRKMTSWPASRMHLTERGAIREGLKADLTIFNYDRIQDTATYDEPLRYPTGI
                     DYVVVNGQLVVEHGRHTGAKPGEVLYGPGHRSGS"
     gene            13377..14063
                     /locus_tag="VFB37_04390"
     CDS             13377..14063
                     /locus_tag="VFB37_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZO21721.1"
                     /translation="MKQKAGRARLGAALKALRRRRGWTLAEVSRRTGFSVPTLSKVEN
                     DKLSLSYDKLIRVSEGCEVDIAQLFAPVEAESAPMTITGRRSINKVAAGELVSTRNYD
                     YLYLSGEVTEKKFIPILAEIRAHSIEEFGKLVRHSGDEFIYVLEGQLEVHTELYAPIV
                     LSKDESAYLDSTMGHAYIALGAGACRVLAVCSAAERELRHAAENRELRDGPSDRADQT
                     KRSRRTVRRR"
BASE COUNT         2640 a         4420 c         4584 g         2480 t
ORIGIN      
        1 ttcgcgccag cgctgcggtt catgcccgaa ccactgtcgc agggcagggc tcggggcgac
       61 ttccttgagc cagagcgtca acgccgctga tttcttcgac actccacgag gccagagccg
      121 gtccacgagc acccgcgcgc catcttgtgg cgagggcggc tcgtaaatcc gcctgatgcg
      181 tgcagctctc atccgctgtc ccatgcagaa gtactgactc atcggcattg tagccggccc
      241 caggcccctg gtgtctggcg ggcgagcacg gacggctttc tcaccgttat cagcgggacg
      301 ctcatcggga aacactggcc tgcgatcggc tcccgtggtc tgatggcctt cgagatgagc
      361 gacccgatac ccgattcgcg tgcccccagg cgccgcagcg caagcagcac ggttggccgt
      421 ggcaaggcca ttcaacacga cgtgctgcca cagcaggcgc gccacgccgc ggcggggatg
      481 gaggtgtcgt ttggcgacag cgcgctgctt tgcgccggct ggccggcgcc ggtttgcatc
      541 gtgagtgatg gcgggtacgg cgtgctcgga ttcgaggggg atacatccga tcatctggac
      601 ttgccgcagt ggtatgaggc tcatgttgcg gcgtgggctt tgcgcgcgac gccgcagacg
      661 actttgtggt tttggaatag cgagatcggc tgggcggcgg tgcatccgct tctggagcgc
      721 tacggctggc gctatgtgag cgccaacctc tggagcaagg gccgggcgca tatcgccggc
      781 aatgtcaata caggccggat ccgccgcttc cccgtagtca ccgagatctg cgtgcagtat
      841 gtcttcgagg ccaaggtgga tgggctcgcg ctgcgcgaat ggctgattcg agagtggaag
      901 cggaccgggc tcccgctgcg cgaagcgaac tcggcctgcg gagtcgccga tgccgccgtg
      961 cgcaagtatc tcaacccggg gcacctgtgg tacttcccgc cgccgcagat gtttgcgcgg
     1021 ttgcaggctt atgccaacga gcgtggggca gtctcggggc ggccatactt cagtctggac
     1081 ggccggacgc ccgcggatga agagcagtgg gcgcgcatgc gcgcgaagtt ccgatgtccg
     1141 ttcggtgtga cgaatctctg ggagcgggga gccttgcgcg gagaagagag gcaggtggtc
     1201 cctggtggac gcgccgtgca cctcaatcag aagcccctgg atctcatgag ccggatcatc
     1261 accgcctgca ctgatcctgg cgatgtcgtc tgggagccct ttggcggtct gttcagcgcg
     1321 accatcgctg cggcccgtgc aggccgcaga gccttcagcg ccgagatcga tcccacctat
     1381 tttcacttcg gcctggagcg catccgggcg gagccgggtt gccgcacctc gaccgggtga
     1441 gcgcactgtg gcgctggagc ctgcttcccg ggcaaaatgc gctcccctcg cgcaaagaag
     1501 aacgccatgc ccaggctcga cacgatgctg agcgctgcca ccctgattgc gctgagcggc
     1561 acaagtggcg caccggcgca gaacgccagg ctcgaagcgg ccgtactgcc gcaaccggct
     1621 gctcccgctg cagcggtact gccgtatgtc agagtgtcgg cgaagcacat catcctgaat
     1681 catgtgcgcg tgattgatgg gatgggcgct gcggaggcgg aggatcgcag catcgagatc
     1741 gcagacggca gaattcgcgc ggtgggcgct gcggtggatt ccccacccgc tgatgcaacc
     1801 gtgctcgagc tgcgcggaca tacggtgatc ccgggccttg tcgggatgca cgaccatatg
     1861 tactacattg cgcgacccaa catggatgcg gatggacaca gcgatccgcc cctcgtcgtt
     1921 ccgcagatga gcttttcggc cccgcggctc taccttgcga acggtgtgac gactctgcgc
     1981 acgacaggca gtgtcgagcc ttatgcagat ctcaacctga aacatctgat cgatgccggc
     2041 gcgttgccgg gcccgcacat ggatgtcacg ggaccctacc tcgagggaag ccactccccc
     2101 ttcatcgaaa tgcatcagct caaggacgcg gatgatgcgc gccgcaccgt ggccttctgg
     2161 gctgaccagg gagtgacctc gttcaaggcc tacatgaaca tcacgcgtgc cgagttgaaa
     2221 gccgcgatcg atgaggctca ccggcgcggc ttgaagctga ccggccatct gtgtgcagtg
     2281 acctatcccg aggcggcggc gctcggaatc gacgacctcg agcacgggtt cttcgtgaac
     2341 acccagctcg atcccggcaa gacgcccgat gtctgtccgc agagcggggg ggtgcccacg
     2401 ctgcgggcga tggatccgga aggtgccccg gctgccgagt tgattcgaac gctgatcgcg
     2461 catcacgtcg ccgtgacttc caccctgccg gtgttcgagc agggcgttcc cgaccatgcc
     2521 ccgctgtggc cccgcgcgat ggaggtcatg acacccgagg cgcgggaggc ctatctctat
     2581 ctgcgtaaca ggaccaacac gctacctgcg gatcaggcgg gcgaacgaag gctgacctac
     2641 cagcacgcac tcggcctgga gcgcaagttc gtggccgccg gcggcctgct gctgggcggt
     2701 ccggatccca ccggcaatgg cggcgtgatt cccggtttcg gtgacctgcg cgagatcgag
     2761 ctcctggtgg aagcgggatt tacggcggcc gaggcggtgc gaatcgcgac attcaacggc
     2821 gcgacctatc tcggtctggc cgagcggatc ggctcggttc agccgggcag ggatgccgat
     2881 ctggtggtgc tgaacgggga tccgggcaag accatcaccg atgtcgagaa tgtcgagctc
     2941 gtcttcaagg atggtgtcgg ctacgactcg gcggcgctgc tgaaatcagt gcgcggccgt
     3001 tatggcgagt actgacggca tcgcgagcta tgagcggtgc gcatccccgt gtggccccac
     3061 gagtggacca cacggggcga tgatcatcgg ccgctgcgat cactggcagt tgaactgctc
     3121 catcgccttg cgcatatcgc ctggcgcgac atccttcacg tgggtcgcga tcgaccagcg
     3181 gtggccgaac ggatcctcga tctgaccata gcggtcgccc cagaacatct gcgtgaccgg
     3241 cattgtgacc ctggcgcctg ctgcagcggc ccttgctgcg aaggcatcgg cgtcctcgac
     3301 gtagaggtgt acggtcaccg gagagccctt gagggccttc gggccgagcg ctccgtgctg
     3361 aggcatttca tccacgagca tcacggtcga atcgccgatg cggatgctgg cgtgcatgag
     3421 cttgcccccg gggccgggca ggcgcgaggt ctccgtggca ccgaatgcct tgcggtagaa
     3481 ctcgatggcc tcgcttgccc cgtcacagat cagatgcggt gtcacggtat gcatgcccgg
     3541 gggaatgggc tgggtgctgg gtgtggacat gaggtctcct ggaatgacgg gtttcgctcc
     3601 aaggacgctc gggcagttcc cgttacgaca acggtgtgga atatttgcgg gatacgagcg
     3661 gtatcttgag cgttcaattt cgacaggctg cactgcagcg cagcccaacc gagaggcagc
     3721 ccatgcggca caggcggtcc ctgagtttca gcgcacttct cctgctcggc gcgctcgccg
     3781 gcggctgtga ccggctcggc atcggggccc acggcgctcc ggtcgcagac ggggcgccca
     3841 ccgagcagga gctgcagaag attcgctata tgagctctgc gccctccgga cccaagggac
     3901 gcacgctcta tacccatttc gaggaagcca agacctgcgg cgatttcgag ctcgcgatgc
     3961 gctggaaccg cccccccaac atcgagagcg gacccttcaa caagaaaatg gtctatgtca
     4021 ccgcatccat tccggctgac ctgccgaaga actccgaggt gttcatccgg ggcaagatcg
     4081 agcagggtga gacgctgccc tcgggcgctg cacgctggta cgtgacgcta caggacggta
     4141 cccgcgtgca ggctatcgaa acggcggact tctatgagaa ggaagagcag gccccgcagg
     4201 acggaggcaa ggccagtgcg ctggtcgagc ccacccgggc tcacaggatc ttctgtgggc
     4261 agggcgtgta cgaaggcctg atcgggaagg atcccgatgg cgacaagaag attcccctgc
     4321 tgtcgatgct ctttgccatg gaccggacga agtgatctcc cggccatagc ctagaaagag
     4381 acgctgttct cttgctgtag aactggcggg tacggcacta tcgtggccga ccaggcgcca
     4441 ctcccgctag catgcgccga cggggccggt ttctcgagga cgcgtcgata atgactcaca
     4501 agagcagcac gctgggtggg ctgatccgcc tgctgcgccg gcagaaaggc tggacgctgc
     4561 ggcagatgag tgaaaaggtg ggtattccac tgtccacgct cgccaaagtc gaggcggaca
     4621 agctctcact cacctacgac aagctgcagc aattcacctc gcgccttggt ctgacgatgg
     4681 ctgagtttct cgcccacgac accactgcag cgcaatcgcc cccacgggtg gtcaccgcgc
     4741 gccgcagcct cacgcacagc ggcaactcca tcccgatctc cacccccaac tatgactacg
     4801 aatacctgtg cgccgatctg cgcgagaagc gcatggtgcc gatcattgcc cgaatccgct
     4861 cgcacagcat cgaggagttc ggcgagaccg tgcggcacca gggcgaggag ttcattttcg
     4921 tcctcgaggg cagcatcgag gtccatctgc agttctacac ctcggttacc ctcaccgcgg
     4981 ggcagggaat ctatctggac agcacgatgg gacatgccta cgtggcaaag gattgcgaaa
     5041 gcgcgctggt gctcgcggtc tgctcgagtg aggatccggt gaatctcgcc agcgagctca
     5101 tgagtctggc cgacagcgag accgagccgg cgatctgagc tcagctgccc gccgcatccg
     5161 agtgccgcag gtagcgcggt ttcaggatcg agacgaggat gatcggcagg atgaagatga
     5221 cgatcagcac ccaggagatg aagcggtagc ctttcgcgat gagctcgacc agcccgatgc
     5281 cctgcgcggc aaacaccgac acggtgagcg cggcgagggt gaccgcgatc cgcacgggaa
     5341 ggtccggatc gtgtccggtt cgcgcgcgca tggcgcggcg cacgcgctcc atgatcgcgt
     5401 gcacgattcc gcagccactc tccagaagcg cgaagaagat catgcactga aacagcagtc
     5461 gcaccagcgg tatgccgatc ctcagaagca ggaagtccga gggcagcgtg ctaccgccca
     5521 cttccgggta ccaggcaatc atcgacagga aaaagaaaag ccccgggagc atcgcaagcg
     5581 gacctgccag cacgccggcg atgacggctt cgcgatgact gcgcagatgc ctgagcaccg
     5641 gcaggatgac gacagccgcg accgagttgt acccggcgta cgttacgcca cccgacagcc
     5701 agccggcgcc gacgtgcgag agctccaggt tcgacaggat gcgggttccg aacttcgaaa
     5761 ggctcagggc aaggaataca gcataagtgg catagagcag gaccgagacg tacttgaata
     5821 ccggctcgac cgctcgcgct ccgaccgcga ccaccgcagc gatcgagacc agcaggagca
     5881 gcgttcccgc cagcgacggc cagccaaaca gcgcatgacc caccgccccg atcgtcgccc
     5941 cgaataccga caggatcagc agaaccagca gcagataggt cagctcgaag aggatccagc
     6001 ccctgcccag cagcttgccg aaaaacgccc gatagtccag caggcggtat gagtacgaga
     6061 acgagaaggt gagtgcgcaa accacactcc acacgatcgt cgcgaccacg atggcgagca
     6121 gtccgccgcg tggaccgctc ggcaggaaga attccgccag ctcgcgcccc gtcgcgtagc
     6181 ccccgccgat gacgacggcc ttgaaggcca ggcccggaag gatgtagcgc tgcagccagc
     6241 tcgactgggc gctctgctga gcggttgaca tggattctct tatcaggaca ttattctttg
     6301 atcaggatat cgtatcacct gccaagggaa cgatgccatt aaggcaggcc gtgagcctgc
     6361 gcagccgacc acccagatga tcccatgaat caacaatcac tcagcaacag gcagctggcc
     6421 tgcgctgcgc tctcgtgcgg cgcgctgctt cagagcgcgt cggtcgccag ccaccaggcc
     6481 gcattcaccc tcgaacaggt catggcggca ccgtacccct cggagctagt gggctcgagc
     6541 accggtaacg cggtcgcctg ggtatacgag acgcaggggc gacgcaacat atgggtcgcc
     6601 gacgccgctc acggcatgca gggccggtcc atcaccaact tcacggcaga cgacggattc
     6661 aacatcggcg agctggcgtg gtcgcacgat ggtcaatgga tcgcatacac ccggggcggg
     6721 tcgctcgagg acgagcgccc ggctaatgtc aacagctccc ctgaaggacc gacgccgcgc
     6781 gaggtatgga tcgtgtccac cgccggcggc gagccgcgca agctcggtgt gggtcattcc
     6841 gccgcgtttt cccccgatgg cgctcgagtc gtgttcgtgg acaaggacaa gctctggtcc
     6901 gtcgcacccg gtgcccgtga ggccgctcag acgctgatcg tggactcggg ccgggtcggc
     6961 tcagtgacgt tctcctccga tgggcggcgg cttgcgttcg tgagctctcg tgggagccac
     7021 tcgatcgtcg gtgtgtatga gtttggaagc cggcatattg tctggctcag cgcgagcctg
     7081 gatcaggatg catcacccgt gttcgcacca ggaggaacgc agctcgcctt catccgcgtg
     7141 ccgtccgaaa agagcccgga attcgttgcg catgcgagcg gcacgccctg gtcgatctgg
     7201 gtggctgata cgctgagcgg gcagggccat cgtgtctgga gggccgatgc gggaatgggc
     7261 agtgtgttcc agccgagcct ctccgagcag aatctgtact ggacgcggcg cgacgagctg
     7321 atatttccgt gggagaagac gggctggctg caactctatg ccgtacctgt ccagggcggc
     7381 ccggtccgga caatcacgac cggtgcattc gaggtcgctc atctggcgct gagcccggat
     7441 cagacgcatc tggccttctc atccaaccag gacgatcagg atcgtttgca cgtctggacc
     7501 gtcgatgccg acagcggcac gccggtgcgg gccgcttccg acaacagcat cgaggacaaa
     7561 ccacaagtga gcgccgaagg cagcgtgttt gccctgcaga gcagtgccaa catgcccttg
     7621 catccggtca tgctcgtcca tggccactgg cagtcgctca tcgagccgcc cgtcccgccg
     7681 atgtttccga ctgacaagct ggtgaggcca gctgccgtga ctttcgcggc gaaagatggt
     7741 cagcaggctc acgcacagct gttcctgcct caggagcgtg gccagtcccg ccaccccgcg
     7801 atcctgttct ttcatggcgg cccgcagcgg cagatgctcc tgggctttca cccgatggac
     7861 gcctacaact ggatgtacgc gctgaatcaa tacttcgtct cggaaggcta catcgttctc
     7921 tcagtcaact accgcggggg aatcggctac ggccacgact atcgcgaggc caaggatttc
     7981 ggacccgccg gcggcagcga gctcaatgat ctgctggggg cgatcgctta cctgcaggct
     8041 cgacaggatg tggacgcgca tcggctgggc atctggggcg gcagctatgg cgggctcatg
     8101 acggcactcg gtctggcacg ggcctccgat tctctggccg ccggtgtgga ttacgccggc
     8161 ctgtacaact gggcctcgtt cctcgcctcg gtcggggcgc cgatcgccag ccctgaggcg
     8221 gtgcagcgcg cgatccaatc ctcgcccgtc gcgaccatcg aacaatggaa gtccccggtg
     8281 ctcatcgttc aggccgatga cgatcgcaac gtgccctcgc aacaggcgtc cgagctgatc
     8341 gcagacctca gatcgcatca catcgagcac gatgagttga tgatgcccaa cgaggtgcac
     8401 gatctgacac gctttgcatc ctggatgact ctgttccacg ccacggacga ctacttcgca
     8461 cgccacctcg gcacgaaggg cttggaaagc ggctcagaga cccgatagag cctgcaggcc
     8521 tcgcgcttgc gaggcgctgt atcaaccgtc cgcagcggtt tgacctggcg gtctgccacg
     8581 ctcgggctcc gctcctcttg ttggtttacc ctgcaaggcc ggtgggtgtt gacctacctg
     8641 accgcacccc tgaggccatg gtatagccag cggtcaggcc ggagctttgt tttggactgc
     8701 caggtctgtc gcccgactgc gacgcgttga cgggtgccag ccccacgaca gaaggtcaga
     8761 ggtgatgagc gtacgcaatc acatcaagct ggtatggctc gttctgacga acaggatgca
     8821 cgtcgagatc cgcaagcacc cgcgtcagga cggcgtaccg cacgaaacgg actccaccga
     8881 catgctgaag catgcgcagg aggcctcgcc tttgtccgga gagggcagtg attacgtcgc
     8941 ccccaacgag gactctgcgc gctcgcgcag cgcctcacga tagtcttgct ggcagctcgt
     9001 ggcccggcca tcgtcccggg ctcttctgtc gccttgcgga gtgttcctaa ccagcgctct
     9061 ggatgcggcg actggccgcc aaggttgccc ctcggcacgc ggcgttttac attttccggc
     9121 tcggaccaac agtgaaacgc aggagcatga gtggtcactg acatcattcc gcgcctgcac
     9181 caagcgctgc cgcaggccaa ggcctggatc gagcgcttcc tggccgccca cgaaagcacc
     9241 gctcagccga tgagctcggt ccgctcagat cgcctggcgg cctgtttccc ggcggatttc
     9301 ctcgctcgga gcaagatcgt ggcggtggat cgggtcgtgt tcccgcccgt cgagagcttc
     9361 ggtctgttcg agtttgcggc gcttcaccag atggaattcg agggaatcac cttcaaggac
     9421 acgatcttcg tgcgccgcga ccgtaagtac gagggtctat atttccacga gctcgtccat
     9481 gtggcgcaat gggcgcgact gggggtggat gacttcctgc tggcctatgc cgcgggtctg
     9541 gcacagcaga gctaccgcga aacgcccctg gagaacatgg cgtatgacct gcaggcgaag
     9601 ttcgaggccg gaacgttgcc gcgggacctg atccagcaga tcgggcatct cacggacaag
     9661 gtgtgggccg atgtgcggca gtttctgaac caggccgacg gtcgggcgcc ctgagccgct
     9721 gcgacttatt atggtaaatc agcggccgcg gtgacccagc gggttgcgtc ctgaaatcag
     9781 ctccgccccg gttgccgatc tgaaggtatc gagcgcactg gcgacgcgtt ctacgagaaa
     9841 cggggaatgc cgtccaaaga ccctgcgcaa agtccaggcc agctccgcac gcgtcgtggt
     9901 gcggctgagg tcgaaggtca gtcgcgagat ttcgttgcga tccttcaggc actcgatgct
     9961 ctgccccatc cacgactgcc gttcggcatc cagagcgcgg cccaggtcgg cgtggaagtc
    10021 cctgtaggct tcgaagcgcg ccgaatgcgg tatgcgtcgt cgaatcatgt ctccagtatc
    10081 ggtcagggcg cgcgcaatct tcagggcatg cttcacaaag cgccattcgt gatcgccttg
    10141 cgagagcctg ccagccccgc cccggctgct tcccggacct ctccactgtt gactctttga
    10201 ccctgagctt ggatcgttgg cgccctcatg tcggcagccc gccccccaga gcctccagat
    10261 cccccgcgct cggtcttcct gagctatgcg tcggaagacc gccaggcggc gcagctcatc
    10321 cgggatgcgc tgccgacgta cgggctcgag gtttggtacg acgagagcga cctgcagggc
    10381 ggagatgcgt gggatcagaa gatccgccgg cagatacgcg aatgcgattt cttcatgccg
    10441 gtgatttcgg ctcgcaccga ggcccgtcac gaggggtatt tccgtaggga gtggcgtctg
    10501 gccgtcgaga ggaccctcga catggccgac gatcacactt tcctgctgcc ggtggtcatc
    10561 gatgagaccc ctcagactgg cgcgcgtgtg cccgaaaagt ttctcgcagt gcagtggacg
    10621 cgggtgcccg gcgggcagcc gagtgcggcg ctcgaggcgc tatgccgccg cctggcctcg
    10681 ggcctgacga tcacgccgga gagccccagg aaagcaccgg atgccttctc cggaacccgg
    10741 gtcgcccccg ccggacggta tcccgaattc ccacgagaag agccgggcca gaaagtgcgc
    10801 ttctgggccc tcgcggccgc atggccgttt caaagcgcct gggtcttctt caaccgcttc
    10861 ccgaagtgga tccggatctg catttacatc tggctgtttg ttctggtgct ctccaaggga
    10921 tgtacctcgt ccgacgacga tcgtccggag aagctcaccc cggcgcaaac gaaaaaactc
    10981 aaacagatct cggacagcta tcaaggcaac gccgacaaag ccgatgtcgc caagctcggc
    11041 gcacagattg cacgggagtt ctcgagggca gccgctggga atgctgcggt cgcaagccct
    11101 ctgctggcga ttcctttcag cgcccgggca ggtgatgcag tcggccgcag ggtcgccgat
    11161 tcgaccttcg cgcaggtgta cgggaggctc gcgatatccc gcggaaatgg cgtcagtctc
    11221 accggcgagc cgcttcccga tcagtcggcg gcgatcgagc ttggacgcgc tcagcacgcg
    11281 aaatacgttc tcttcggaac cgtcgaaggc caacccggtg cgcaggagct tgccgtgaag
    11341 atgctggcgg taacggatgg cacggttctc tggtcgaagt cgtatccggc caagggggcg
    11401 gatcctgcaa cgatcgctgc agaggtgaat gccaaggtcc tcagtctgga cgaggattga
    11461 ggctctgcgg agaagcccgc tgtcgtgaga accctggcgc gcctggagtt tgcctcgagg
    11521 cacccctatc cctgacccgc gccgtagttt cagttttgga aaattatttt ccgatatggt
    11581 atggtcgccg ccgccgtgag ccatttgcaa agcgactcag ttgcggggcg acctcatgaa
    11641 gaattggatt ctcgccatgg caatcctcct cgcagctgcg ccggctgcgg ctgatcagcc
    11701 cacgtacgat ctcgtgatcc gcaatgctcg cgtgctcgac ggcgagggca accctgaggt
    11761 gcacgccgac atcgccatca gcggcggtcg ctttgtgcga atcggacgcg tcaccggcca
    11821 cggacggcgc gaaatcgacg tgcagaacga ttacgcatcg cccggttgga tcgacatgat
    11881 ggaccaatcc ggagaggtcc tgatccgcaa cgggctggcg gagaacaagt tgcgtgaggg
    11941 ggtgaccacg gcgatcgcgg gcgagggggg tgctccggtg ccctctgcga aacttaccga
    12001 ctattttcac ggactggcgc agaagggcat ttcactgaac ttcggcactt actacggcgc
    12061 agcgcaggca cgcgtcgagg tcatggggga cgtcgccggc gagccgactc cgcagcagat
    12121 ggatgcgatg aaggcgcatg tggatgaggc catgcgcgcc ggggcaatgg gcattgcgac
    12181 tgccttgatc tatccgcctg acagctttca gagcacctca gatctgatcc agctggcgcg
    12241 ggtcgccgcg cagtacgacg gcatgtacgc cagccacatg cgcgatgaga gcgccaatct
    12301 gctgaaggcg atccgcgagt cgatcgagat cggtgagcag gcggggatcc ccgtggagat
    12361 ttttcacttc aaggtggctt acgcccccgg gtggggcaag ctcgtcaacg aagccgggca
    12421 gctcatccgc gacgcccgcg cgcgcggtgt caacattgcc tcggacatgt atgtctacac
    12481 ggcgggcggt accggactcg acatcacagt gccgaactgg gtatgggaac aaggcgagaa
    12541 gaaggggctg gaacgactga aggagcgaca catccgcgag cgactgaaac gggaagtggc
    12601 cgcaggctcg cagcccggtt ggtccaatct gatcgaagcc agcggcggct gggaccatgt
    12661 ggtgcttgcc aatgccttca atcccaaata cgacgcgtat cgctacaaga gtctggccta
    12721 catcggccag cagctcggca aggatcccgc ggatgttgca tgggacatcg tgctggcggc
    12781 gcagccgaat cgggccatgg cgctgtactt catgatgagc gagcaggaca tcgaaaccgc
    12841 cttgcgctgg ccctggatga gtatcggcag cgatgcgggt gcgaatgaga aggaaggcca
    12901 gatagatgcc atcggtctgc ctcacccgcg cgcgtatggc aacttcccgc gcgtgattgc
    12961 cgagtacgtg aagaagcgtg gcgtactgac gctagaggac gccattcgca agatgacttc
    13021 gtggccggcg tcgcgaatgc atctcaccga gcggggcgcc atacgggaag ggttaaaggc
    13081 tgacctcacg attttcaact acgatcgaat ccaggacacc gcgacctacg atgagccgct
    13141 gcggtacccg acgggtatcg actacgtggt cgtcaatggc cagctcgtgg tggagcacgg
    13201 gcgccatacc ggcgccaagc ccggcgaagt gctctatggc ccgggccatc gctccggaag
    13261 ttgagacggc agtcctccag ggaagcaggg gtcgtgccct tccagggtgg ttcggcaggc
    13321 gggatgggcc cagaagccgg tacactgccc aggcaggagc tccaacgttg ccgagagtga
    13381 aacagaaggc gggccgagcg cgcctgggcg cagccttgaa ggctctgcgc cggcgcaggg
    13441 gctggaccct ggcggaagtc agccgccgca ccggcttttc ggtccccacc ctctcgaagg
    13501 tggagaacga caagctctcg ctctcctatg acaagttgat tcgcgtcagc gaaggttgcg
    13561 aagtcgacat cgcccagctg ttcgcgccgg tggaagcaga atcggcgccg atgacgatca
    13621 cgggccgccg cagcatcaac aaagtcgctg ccggcgagct ggtctcgacg cgcaactacg
    13681 attatctgta tctcagcggc gaggtgacgg aaaagaaatt cattccgatt ctcgccgaga
    13741 ttcgcgcaca ttcgatcgag gaattcggca agctggtgcg ccactcgggc gatgagttca
    13801 tctacgtcct cgaaggccag ctcgaggttc acaccgagct ttatgcgccc atcgtgcttt
    13861 ccaaggacga gtcggcctac ctggacagca cgatgggtca tgcgtacatc gccctcggcg
    13921 cgggcgcttg ccgggtactg gcagtctgtt cggcggccga acgcgaactg cgccacgcgg
    13981 ccgagaacag ggaactccgc gatgggccga gcgaccgtgc cgatcagacc aagcgatctc
    14041 ggcgaaccgt ccgccgacgc tgacgcggac aatttgcgcg ccggtggcgc ggcgagcgcc
    14101 attgaagtcc aagcgctcgg gacg
//
LOCUS       DASRHN010000117        17253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1686795, whole genome shotgun sequence.
ACCESSION   DASRHN010000117 DASRHN010000000
VERSION     DASRHN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686795"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="VFB37_04395"
     CDS             <1..1976
                     /locus_tag="VFB37_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HZO21722.1"
                     /translation="LTCRVGFAIAALLTALQCDALGNGVTPYLPLNLEPEIESQIERV
                     LILGDQPVMTRPIPAATVLDALPKACAIDEALCQRVRRYLARYMYGSGLAEASLEGAL
                     SSGSGDRTVTPNRYGMREDSHWQASAQAYAQPSDYVLIDAGAVAYEGRTTFTGSMLSL
                     GWDFAQLDIGFRPHWFSPLSDSSMLMSTEAPTMPSITLSNYRQFFGLGLHYELFAARM
                     SNSDRIVDGTTLTAGHPRLAGFHLDVEPASGWSLGLSRLLQYGGGALGGSFRDLLDAF
                     FSPSAAQTSNVSHPQPFGNQEASITSSLLFPGKIPFSVYFEYAGEDTSRGRDYLLGNS
                     SLSAGIHFPSLWKHFDLTLEASEWQNAWYVHTVYLDGMTNYGLVTGNWFGDQRVFGDG
                     VGGHSSMVALGWEPPFGGLVQLRYRTLQNQEYGSFEDYQRYQFVSLGYSRPLLGLVVG
                     GELDEGRDVFGEKFTRLAGFVRYDEGAALSSLPSSQEDDEDEGAPKSGEVFIDTGANV
                     SRQNIDLTSAATRHNGPVDSGYHLGLGARRFVSDHNDLGARLEFDNVQGHSLIGARLI
                     DYRYRFNNPLTFNFFLGAARYDLATPAYGFYYGVGTQWRDILPKWDLGADLRYANSIA
                     RDHLLPSDPPSVGGRPDSFYNISSVTIFISRRF"
     gene            complement(2030..3673)
                     /gene="pgi"
                     /locus_tag="VFB37_04400"
     CDS             complement(2030..3673)
                     /gene="pgi"
                     /locus_tag="VFB37_04400"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZO21723.1"
                     /translation="MQTTPGPRTSEWADLTAQATRLNDVAISELFSRDPGRYERFFRQ
                     AAGLHMDFSRQRIDEIVLGKLMELADAAGLRQRIEAMWRGEHINVTEDRAVLHVALRQ
                     PPGAGIGGEAIEQEVMTERERMLSFAEAVRTGQIVGSARKPFTLIVNIGIGGSDLGPA
                     MAVEALQRYAAGGPRCEFVSNVDGNHLHDVLDGADPATTLFIVASKTFVTLETLTNAR
                     TARGWLKAKLGEEAVPTHFAAVSVNHQAMDEFKVNPDYRFQMWDWVGGRYSIWSSIGV
                     SLAIAIGREHFLEFLRGGHELDEHFRSTPWQSNLPALAGLAGIWNINFMNLPTHAVLP
                     YDDRLRRLPAYLQQLEMESNGKSVTIEGSAVQWGTAPIVWGEPGDNAQHSFFQLLHQG
                     TPRAALDFLLPARSSCADQNHQNLAIANCLAQAEAFMNGRSAEEVRAELARQNVPPAR
                     IDALTPQKVHQGNRPSTILLFARLNPATLGKIIAFYEHKVFTQGVIWGINSFDQWGVE
                     LGKKLAEQLTPAVQDPGAHPAPAAVAKLLACISRLRDQH"
     gene            complement(3725..4903)
                     /locus_tag="VFB37_04405"
     CDS             complement(3725..4903)
                     /locus_tag="VFB37_04405"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZO21724.1"
                     /translation="MTSRIQVPLLDLKPQYQPLAEEIQAAIARVCASQHFILGPAVKE
                     LETNVAALSQCRFGIGVSSGTDALLLALMALDFGPADAVITSPFTFFATGGTIARTGA
                     APLFCDIDPVTFNLSPEAVRACLQTRCERRSGELFTPKGQRVRAIMPVHLYGQLADMP
                     PLVEIAQANGLRMIEDAAQAIGAQDRHGRRACSYSDVGCLSFFPTKNLGAFGDAGMCV
                     ANDPTIAERLEIMRVHGGKPKYFHSVIGGNFRMDELQAAILNVKLKHLEGWTAMRQRN
                     AAFYDGAFAAADLGRAVQTPAAVAGGRHIYNQYVIRVRDRDLLRQHLTANGVGTEIYY
                     PLPLHLQKCFAYLGHRAGDFPVSERAAEETLALPIYPELTETQLQYVVDTIAAFYSRG
                     "
     gene            complement(4900..5916)
                     /locus_tag="VFB37_04410"
     CDS             complement(4900..5916)
                     /locus_tag="VFB37_04410"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HZO21725.1"
                     /translation="MKILLTGAAGFIGYHTSLRLLDKGYEVVGLDNLNEYYDINLKKA
                     RLAQLQKRAAFRFEKLDLTDREGVAALFAREKFPRVIHLAAQAGVRYSLEAPHAYVDS
                     NITGTLNILEGCRHNQVAHLVYASTSSVYGANTAMPFSVHQIADHPLSLYAASKRANE
                     LMAHNYSSLFKLPTTGLRFFTVYGPWGRPDMALFLFTRNILEGKPIDVFNHGRHKRDF
                     TFVEDIAEGVVRVCERIPKADPAWSSDSPDPATSNAPFRLYNIGNNQPVELMRYIEVI
                     EECLGRKAEKHFLPMQLGDVPETYADIDDLVRDVGYRPATPIETGVRRFVEWFCDYYG
                     YTKR"
     gene            complement(5913..7193)
                     /gene="tviB"
                     /locus_tag="VFB37_04415"
     CDS             complement(5913..7193)
                     /gene="tviB"
                     /locus_tag="VFB37_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HZO21726.1"
                     /translation="MPLNLRKCRIGVVGLGYVGLPLAVEFGKHFQTLGFDVKAPRIRE
                     LEAGHDSTLEISRAELKSAKYLRFTTDLAAMKSCRVYIVTVPTPIDQYKRPDLTPLIR
                     ASESVGKVLKKDDVVVYESTVYPGCTEEVCVPILERESGLRLNKDFFAGYSPERINPG
                     DKEHRLTTIRKITSGSTPQAAEFVDQLYSRIIKAGTHRASSIRVAEAAKVIENTQRDV
                     NIALINELALIFNRLGIDTEEVLNAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKAQE
                     IGYNPEMILAGRRLNDNMSLYVAGEITKLMARKRIHVNGCRALVLGITFKENCPDIRN
                     SKVVDVIRELQKFGAQVDVHDPWADGAECRHEYGLRPVRKLKPNSYDVAVIAVAHRQF
                     RELGAQGVRRLCRKNHVVYDIKHVFAATEVDGRL"
     gene            complement(7246..7995)
                     /gene="dnaQ"
                     /locus_tag="VFB37_04420"
     CDS             complement(7246..7995)
                     /gene="dnaQ"
                     /locus_tag="VFB37_04420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZO21727.1"
                     /translation="MRQIVLDTETTGLEPELGHRIIEIGCVELVNRRPTGRTFHRYLN
                     PERDIEEGALGVHGISREDLLDKPRFMDVADGLIEFIGGAELVIHNAAFDVAFLDAEL
                     ARVQGEARTISGVCSVLDTLALARSMHPGQRNSLDALCKRYSVDNSSRELHGALLDAR
                     ILADVYLAMTGGQGALALSDAIAMPGTAETVRTVRALVRPPIPLVIISASEEEMAAHA
                     AMLQIISAASGGACLWLREAGETPEMNRSSA"
     gene            complement(8007..8468)
                     /gene="rnhA"
                     /locus_tag="VFB37_04425"
     CDS             complement(8007..8468)
                     /gene="rnhA"
                     /locus_tag="VFB37_04425"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZO21728.1"
                     /translation="MSLTQQPAAQVVEIYTDGACRGNPGPGGWAATLRFADSEKELAG
                     AEALTTNNRMELTAVIRALEALKRPVRASVFTDSEYVRRGIKEWIHSWKARGWRTADR
                     KPVKNQDLWERLDELAAVHEIEWHWVRGHSGVPGNERVDQLANQAIDALLR"
     gene            complement(8465..9217)
                     /locus_tag="VFB37_04430"
     CDS             complement(8465..9217)
                     /locus_tag="VFB37_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO21729.1"
                     /translation="MPVRISRIPDRDAALSSWWASPLGRALLAAESELLGEALEDVFG
                     WELLQIGAWGGSRELLACSRTRRQTIVAAPAFPCGADIIGRPSLLPINSDSVDAVLLP
                     HTLEFAADQYAIVREVDRILVGEGQLLVLGFCPWGLWGMRARASRDGFPPGMRRVLSE
                     RRVRDWLVLLGYEVVASRRYLYCSPWSSRRAAGEGVGRILRRGFTYPFPGSAYLLKAR
                     KRVYTLTPVRPRFREKPAVLGGLVKPTTRSQS"
     gene            9345..10976
                     /locus_tag="VFB37_04435"
     CDS             9345..10976
                     /locus_tag="VFB37_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZO21730.1"
                     /translation="MAREPILKTRWVLVLSALVLISGCAHAPRRPVTPAAPAADAVPP
                     VEPSSTYPEAPTPPAEQAPAPEDATLAQQPTQVAGLTPAQYADLFDRMRAGFHIEDAD
                     RAMIDQQLAWFASHPDYLERAFGRAELYLYHIVTELEARGMPLEIALLPVVESAFEPY
                     AYSRARASGLWQFIPGTGSRYGLRQDWWYDGRRDVVESTRAALDYLQSLHDEFNGDWL
                     LAIAAYNCGELAVEHALDVNRQKGKPLDFWSLKLPKETQAYVPKLLAMRRLVLDPEKY
                     DISISPIPNQPYFTRVDTRGQIDLKLAAEIAGVTDEEIYELNPAYHRWATDPAGPHYL
                     LLPIDGAQTFSQNISQLTDDQLMRATSYTVAKGDSVISVARRFHTTPDVIREMNELPS
                     GPLTVGADLRVPSTSVTLPAKVLLAAARVDGHDRFARRPHVHVVHRGDSLWSIARRTG
                     MNVNTLALMNGMQPGDPLRAGQRLRLSNRGTASTGSAKSSRQVTYTVRSGDTLTQIAR
                     LFQVSVKQILAWNGMISRALTPGQKLTIRVLSRGG"
     gene            11084..11881
                     /locus_tag="VFB37_04440"
     CDS             11084..11881
                     /locus_tag="VFB37_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HZO21731.1"
                     /translation="MGFLTGKRAFIVGVATDRSIAWGIAQALHREGAELAFSYVNDKM
                     KERVEPLAQSLGSTLIMPLDVTIDGQLDSAFELIRREWGQLDIVIHAVAYAPREAIMG
                     SFVDNTSREAFRIAHDVSSYSLTALARGAKPLMSGRKGALLTLSYLGAVRSIPSYNVM
                     GLAKASLEANVRFLAADLGPAGIRVNGISAGPIKTLAAAGIAGFRKMLSRVAEIAPLR
                     RNVTPEDVGNAAAFLCSDLAAAITGEILYVDNGFSTVGMSFSEQGND"
     gene            11886..12818
                     /locus_tag="VFB37_04445"
     CDS             11886..12818
                     /locus_tag="VFB37_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO21732.1"
                     /translation="MARRIFITGAAGLVGQNLIAHIEQQGPAEIVGLDKHAHNLAVLA
                     RLHPDVRLIESDCATSGEWEKVLGECDELILLHAQIGGLSAAEFERNNVAATRRVLEA
                     AARGRVSHIVHVSSSVVNSRAQDLYVQTKKQQEALVSASAIPHVILRPTLMFGWFDRK
                     HMGWLMRFLRRAPLFPIPGSGKYLRQPLYAGDFCAIVLACMQRGMVGEYNISGLSRID
                     YIDLIRELRRVSGARTPIVRLPYSVFRLLLATYALFDRDPPFTTAQLRALVIPELFEV
                     IDWPAIFQVTPTPLHEALERTFNDPRFADVALRF"
     gene            12799..14127
                     /locus_tag="VFB37_04450"
     CDS             12799..14127
                     /locus_tag="VFB37_04450"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO21733.1"
                     /translation="MSHFDSEPHARDLLVIGAGPMGLAAAYFACARGYRVEVLEAGDR
                     PGGMAAHFDFGGLSLERFYHFCCKTDVDTFALLSELGLPAAVHWVPTRMGYFVAGRLH
                     PFGDPVALLRFPWLSLVEKMRYAAMAFCATKRSDWSALDRLSARDWLVRWCGPRAYDR
                     MWRPLFELKFFEFANSISAAWAWQRIKRLGKSRRSLFQEELGYIEGGSEALIERLVDA
                     IERGGGRIRLKEPVRHILVEDGTVKGVETAAAQRAPAAAVISTVPLPYVPAMLQRDAP
                     QLAARYTGLDNIAVVCVVHKLRRSVSPHFWVNICDPDIGIPGFVEFSHLRPLPDTIVY
                     VPYYMPATRERFSWSDEALGDESFRFLQRINPALTAADRIDTHVARLRYAQPVCTVGF
                     ASKLPPAQTSIAGLQIADTSFYYPEDRGISESIKFARRMVQALPSHAKAG"
     gene            complement(14191..14859)
                     /locus_tag="VFB37_04455"
     CDS             complement(14191..14859)
                     /locus_tag="VFB37_04455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO21734.1"
                     /translation="MDLQQLYLNRFDETARARKAVVWDVLYRHVFQQWIRPTDTVLDL
                     GAGFCEFINSARAGRRIAVDLNPDTARLAAPGVEVQACSATELGFLGPGAVDVVFSSN
                     FLEHLSCKQDVASALSESMRVLRPGGTLILLGPNVRLIPGPYWDFFDHHVPLSDRSLC
                     EVLSVTGFDLVRVEPRFLPYTTRSALPQAAWLVALYLRLRPLSSRFLGKQFLIVARKP
                     SAAR"
     gene            complement(14868..15350)
                     /locus_tag="VFB37_04460"
     CDS             complement(14868..15350)
                     /locus_tag="VFB37_04460"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZO21735.1"
                     /translation="MSTPPLARSPGAASAGPTSAPAVGDARADSQPLRRQFRNFLLAG
                     GVAAAANFGSRFIFSLWVRYEWAVVLAFVVGLATGFVLMRAHVFGGQGKAIGPQAARF
                     VVVNLLALAQTLAISVALARWALPAVGLQAHAQALGHLAGVTVPMLTSYFGHRLFTFR
                     "
     gene            complement(15347..16090)
                     /locus_tag="VFB37_04465"
     CDS             complement(15347..16090)
                     /locus_tag="VFB37_04465"
                     /inference="COORDINATES: protein motif:HMM:NF005489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO21736.1"
                     /translation="MTQNVVIVGATSGIAQAVGRCYAARGSRLFLAARNPQRLEAVAA
                     DLRARGAGAVDIFIMDAEDAAQIQAMEQSAWAALGKVDVALIAYGSLPDQARAAVDST
                     YLVREWRVNAESVLLCLAGLAQRFQSQGGGIIAVVGSVAGDRGRPGNYLYGAAKSAVH
                     AFSSGLRAQLWAQRVHVLTIKPGFVATSMTAHLNLPRLLTAEPAAVARRIVRAIDRRW
                     DVCYVPGFWRLIMTVIRLLPETIFKRLKL"
     gene            complement(16087..>17253)
                     /locus_tag="VFB37_04470"
     CDS             complement(16087..>17253)
                     /locus_tag="VFB37_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO21737.1"
                     /translation="YGDACLAVSDHVLMMTNMNRILAADWQSGRIVAEAGLTLAELIE
                     VALPRGWFPPVTPGTKFITLGGAVANDVHGKNHHVMGTFGRHVGSLTLYRTDTGASTI
                     SRTQAPELFAATIAGLGLTGVILSVELQLRPVSSARIQERRIRFRGLDEFFDLVAGYD
                     QDYEYTVAWIDCAASGKTAGRGHLIVGNHAPHGALRCEQPGALKVPIDPPFSLVNPFT
                     LRAFNAVYYRSQLRREVQRSAGYDSFFYPLDRISGWNRMYGRAGFQQYQCIVPDATSR
                     ESIRSVVRAIARSGTGSFLAVLKKCGALTSPGLLSFPMPGVSLALDFPQSARTVALFG
                     ELDALVHDAGGRLYPAKDAHMTAGHFQSAYPAWRSVEALRDPRLMSRFWQRVTQ"
BASE COUNT         3015 a         5620 c         5539 g         3079 t
ORIGIN      
        1 ggctcacgtg tcgcgtcggt ttcgctatcg cggcactcct taccgcgctc cagtgcgatg
       61 ccctgggtaa cggggtcacc ccctacctgc ctttgaatct cgaacctgag atcgagagcc
      121 agatcgagcg ggtactgata ctcggagatc agccggtcat gacccggccg attcccgccg
      181 ccacggtgct cgatgcgctt ccaaaagcgt gcgcgatcga tgaggctctc tgccagcgcg
      241 tccggcgcta cctcgcgcgc tacatgtatg gctcgggact tgccgaagcc agcctcgagg
      301 gtgcactgag cagcggctcc ggcgaccgta cggtgacccc caatcgttac ggaatgcgcg
      361 aggacagcca ttggcaggct tcggcacagg cctatgccca gcccagcgac tacgtgctga
      421 tcgatgcggg agccgtcgcc tacgaaggtc ggacgacctt caccggctcc atgctgagcc
      481 tgggctggga tttcgcgcag ctcgatatcg gctttcgtcc gcactggttc tcgccgctga
      541 gcgacagcag catgctgatg agcacggagg cgccgaccat gccctccatc accctgtcga
      601 actaccggca gttctttggg ctcggcctgc actacgagtt gtttgccgcg cggatgtcga
      661 attcggaccg catcgtggat ggcacgacgc tcaccgcggg gcatccgcgc ctggcggggt
      721 ttcacctgga tgtcgagccg gcctccggct ggtcgctggg tctgagccgg ctcttgcagt
      781 acggcggcgg tgcgctcggc ggctcgttca gggacctgct ggatgccttc ttcagcccga
      841 gcgcggcgca gacctccaac gtgagccatc cacagccctt cggcaatcag gaggcttcca
      901 tcaccagcag tctgctgttc cccgggaaaa ttccgttctc ggtgtatttc gagtatgcgg
      961 gcgaggacac ctcccgcggc agggactatc tgctgggcaa ttcctcgctg tccgccggca
     1021 tccatttccc cagtttgtgg aagcatttcg acctgacgct cgaggcgagc gaatggcaga
     1081 acgcttggta cgtccacacg gtgtatctgg acgggatgac caactacggg ctcgtcaccg
     1141 ggaactggtt cggggatcag cgcgtcttcg gcgatggggt cggcggtcac agcagcatgg
     1201 tggctttggg gtgggagccg ccctttggcg gcctggtgca gctgcgctat cggaccctgc
     1261 agaaccagga gtacggcagc ttcgaggact atcagcgtta tcagttcgtc tcgctcggct
     1321 attcccgccc gctgctgggc ctcgtggtgg gcggggagct cgacgaaggt cgtgatgtct
     1381 tcggcgagaa attcacgcgc ctggccgggt tcgtgcgcta cgacgaaggt gccgcgctca
     1441 gctcgctgcc gagctcccag gaagacgatg aggatgaggg ggcgccgaag tccggcgaag
     1501 tcttcatcga cacgggcgcc aatgtcagcc gccagaatat cgatctcacc tcggcggcga
     1561 cccgccacaa cggcccggtg gactccggct accacctcgg gctgggtgcg cgacgcttcg
     1621 tctcggacca caacgatctg ggtgcccggc tcgagttcga caacgttcag gggcacagcc
     1681 tcatcggcgc gcgcttgatc gactatcgtt accggttcaa caatcccctg accttcaatt
     1741 ttttcctggg tgccgcgcgt tacgatctcg ctacccccgc atacgggttc tactacggcg
     1801 tgggcactca gtggcgcgat atcctcccga agtgggatct gggcgccgac ctgcgctacg
     1861 ccaacagcat cgcgcgcgat catcttctgc caagcgatcc ccccagcgtc ggggggcgac
     1921 cggacagttt ttacaacatc tcgagcgtga cgatcttcat cagccgccgc ttttgagacg
     1981 cgagcgctct gccgccacac ggtagcaatc agtcacactg cgacggccct cagtgctgat
     2041 cgcgcagccg gctgatgcag gcgagcagct tcgccaccgc tgcaggcgcc ggatgcgcgc
     2101 cgggatcctg caccgcgggg gtcagctgtt cggccagctt cttgccgagc tcaacacccc
     2161 actggtcgaa ggagttgatt ccccagatca ccccctgcgt gaagaccttg tgctcgtaga
     2221 aggcgatgat cttgccgagc gtggccggat tgagccgcgc gaacagaaga atggtgctcg
     2281 ggcgattgcc ctgatgcact ttctgcggcg tcagtgcgtc gatgcgcgcg ggcggcacat
     2341 tctgccgggc gagctccgcg cggacttcct ccgcggagcg gccgttcatg aacgcctccg
     2401 cctgcgcaag gcaattggca atagcgaggt tctggtggtt ctggtctgcg cacgaggagc
     2461 gcgccgggag cagaaaatcc agggcggcac gcggggtgcc ctggtgcagg agctgaaaga
     2521 acgaatgctg cgcgttatcg cccggctcgc cccagacgat gggcgcagtc ccccattgca
     2581 ccgcactgcc ctcgatggtg acgctcttgc cgttactctc catctcgagc tgttgcagat
     2641 aggccggcag gcgccgcagg cgatcgtcgt agggcagcac ggcgtgggtc ggcagattca
     2701 tgaagttgat gttccagatg ccggcgagcc ctgccagcgc cggtagattc gactgccagg
     2761 gcgtgctgcg gaaatgctcg tcgagctcgt ggccgccgcg caggaactcg aggaagtgct
     2821 cgcgtccgat ggcaatggcg agcgacacgc cgatcgagga ccagatcgaa taacggccgc
     2881 ccacccagtc ccacatctgg aagcgatagt ccggattgac cttgaactcg tccatcgcct
     2941 ggtgattgac cgacacggcc gcgaagtgag tggggacggc ctcttccccg agcttcgctt
     3001 tcagccagcc gcgcgcggtt cgcgcgttgg tcaaggtctc cagcgtcacg aacgttttgg
     3061 aggccacgat gaagagtgtc gtggcgggat ctgcaccatc gaggacatca tgcaaatgat
     3121 tgccatcgac gttggaaacg aactcgcagc gcgggccgcc cgcggcgtag cgttgcagag
     3181 cctcgaccgc catggccggc ccgaggtcgg agccgccgat gccgatgttg acgatcagcg
     3241 tgaacggctt gcgggcgctg ccgacgattt gccccgtgcg caccgcctcg gcaaacgaga
     3301 gcatgcgctc gcgctcggtc atgacttcct gctcgatggc ttccccgccg atgcccgccc
     3361 cgggcggctg ccgtaaggcg acatgcagca cggcccgatc ctcggtgaca ttgatgtgct
     3421 caccgcgcca catggcctcg atgcgctgac gcaagccggc ggcatcggcc agctccatca
     3481 gcttgccgag aacgatctcg tcgatgcgct ggcgggagaa atccatgtgc aggccggccg
     3541 cctggcggaa gaagcgttcg taacgtcccg gatcccgact gaagagctcg gagatcgcaa
     3601 cgtcgttgag gcgcgtggcc tgagctgtca gatccgccca ttcgctcgtg cgcgggccgg
     3661 gggtggtttg catcggagga tctcctgcgg acgaggcggc ggggccgtgc ccgcgcggcg
     3721 cgagttagcc gcgactgtag aaagcggcaa tcgtatcgac gacgtattgt aattgggttt
     3781 cggtgagctc cgggtagatc ggcagcgcga gcgtctcctc ggcggctcgc tccgaaaccg
     3841 ggaaatctcc cgcccggtgc ccgagatagg caaagcattt ctgcaaatgc aacggcagcg
     3901 ggtaatagat ctcggtgccc accccgttgg cggtgagatg ctgtcgcaac aggtcgcgat
     3961 cgcgcacacg aatcacgtat tgattgtaga tgtggcgtcc gccggcaacc gcggcgggcg
     4021 tctgcacggc gcggccgaga tcagccgccg cgaacgcgcc gtcatagaag gcggcattgc
     4081 gctgtcgcat cgccgtccat ccctccagat gcttgagctt gacgttcaga atcgcggcct
     4141 gcagctcatc cattcggaag ttgccgccga tgaccgaatg gaaatacttc ggcttgccac
     4201 cgtgcacgcg catgatttcg aggcgctcgg cgatggtcgg gtcgttggcg acgcacatgc
     4261 ccgcatcgcc aaaggcgccg agattcttgg ttgggaagaa ggagaggcaa cccacatcgc
     4321 tgtagctgca ggcgcgcctg ccgtgccgat cctgagcccc aatggcctgg gccgcatcct
     4381 cgatcatccg cagcccgttc gcttgggcga tctccaccag tggtggcatg tccgcgagct
     4441 gtccgtacag atgcaccggc atgatggccc tgacgcgctg ccccttgggc gtgaacagct
     4501 cgccggaccg ccgctcgcac cgagtctgca ggcatgcgcg caccgcttcg ggcgacagat
     4561 tgaaggtcac gggatcgatg tcacagaaca gaggcgctgc accggttcgc gcgatcgttc
     4621 ctccggtcgc gaagaaggtg aagggactgg tgatgaccgc atccgcgggg ccgaagtcga
     4681 gggccatcaa cgcaagcagc agcgcatccg tgccggagga gacgccgatc ccgaagcggc
     4741 attgactcaa ggcggcgaca ttcgtctcga gctccttcac ggcaggtccg agaatgaagt
     4801 gctggctggc gcagacccgg gcgatggcgg cctggatctc ctcggccagc ggctggtact
     4861 gcggcttcag atccagcagc ggaacctgta tgcgggaagt catctcttcg tatacccgta
     4921 gtagtcgcag aaccactcga cgaagcgccg cacgcccgtc tcgatcggcg tggcgggccg
     4981 gtagccgaca tctctcacca ggtcatcgat gtcggcgtat gtctcgggca cgtccccgag
     5041 ctgcatgggc aggaagtgct tctcggcctt gcggcccagg cactcctcga tgacttcgat
     5101 gtagcgcatg agctcaaccg gctggttgtt gccgatgttg tagagcctga acggggcatt
     5161 gctcgtggca ggatccggtg agtcgctgct ccaggcggga tcggccttgg ggatgcgctc
     5221 gcagacccgc accacgcctt cggcaatatc ctcgacgaag gtgaaatcgc gcttgtggcg
     5281 cccatggttg aacacatcga tcggcttgcc ctcgagaatg ttgcgcgtga acaggaacag
     5341 ggccatgtcc gggcggcccc agggtccata gacggtgaag aagcgcaggc ccgtggtcgg
     5401 cagcttgaac agggaagaat agttgtgcgc catcagctca ttggcgcgct tgctcgcggc
     5461 atacagcgac agaggatggt cggcaatctg atgcacactg aagggcatcg ccgtgttggc
     5521 cccgtacacc gagctggtcg aggcatacac cagatgcgcc acctggttgt gccggcagcc
     5581 ttccaggatg ttgagcgtgc cggtgatgtt gctatcgacg taggcatgcg gtgcctcgag
     5641 cgagtagcgc acaccggcct gcgcggccag gtggatgacg cgcgggaatt tctcgcgggc
     5701 gaacagggcg gccacgcctt cccggtccgt caggtcgagc ttctcgaagc ggaaagcggc
     5761 ccgcttctgc agttgtgcga ggcgcgcctt cttcagattg atatcgtaat actcgttgag
     5821 gttgtcgagt ccgacgacct cgtacccttt atcgagcagc cgcaggctcg tgtgatagcc
     5881 gatgaagccc gcggcacccg tgaggaggat tttcatagcc tgccgtccac ttccgttgcc
     5941 gcaaagacgt gtttgatgtc gtagacgacg tgattcttgc gacacagccg ccgcaccccc
     6001 tgcgcaccca gctcgcggaa ttgccggtgc gcgacggcga tcaccgcgac gtcatagctg
     6061 ttcggcttga gcttgcggac cggccgcagg ccatattcgt ggcgacattc cgctccatcc
     6121 gcccacggat cgtggacgtc aacctgggct ccgaacttct gcagctcgcg gatcacatcc
     6181 accaccttgg agttgcggat gtcgggacag ttctccttga acgtgattcc gagcaccagc
     6241 gcgcggcagc cgttgacgtg aatgcgcttg cgggccatga gcttggtgat ctccccggcc
     6301 acgtacaacg acatgttatc gttcaggcgg cgcccggcga ggatcatctc ggggttgtag
     6361 ccgatctcct gggccttgtg tgtcaggtag tacggatcga cgccgatgca gtggccgccc
     6421 accagccccg gccggaaggg caggaaattc catttgctgc cggcggcatt caacacttcc
     6481 tcggtgtcga tgccgaggcg attgaagatc agagccagct cgttgatcag cgcgatgttg
     6541 acatcgcgct gggtgttctc gatgaccttg gcggcttcgg ctacgcggat gctggaggcg
     6601 cggtgcgttc ccgccttgat gatgcgcgag tagagctggt cgacgaactc ggcggcctga
     6661 ggggtcgagc cggaggtgat cttgcggatg gtcgtcaggc ggtgttcctt gtcgccggga
     6721 ttgatccgct ccggactgta gccggcgaag aagtccttgt tcagccgcag tcccgattcg
     6781 cgctcgagga tcggcacgca aacctcttcg gtgcagcccg gatagaccgt ggactcgtac
     6841 acgacgacgt catctttctt gagcaccttg ccgacgctct cgctggcgcg gatcagaggt
     6901 gtcaaatccg gacgcttgta ttggtcgatc ggggtgggca ccgtgacgat gtagacccgg
     6961 cagctcttca tggcagccag atccgtcgtg aagcgcagat acttcgcgct cttcagctcc
     7021 gctcgcgaga tctccagcgt gctgtcgtga ccggcctcca gctcgcggat acgcggcgcc
     7081 ttgacatcaa agcccagcgt ctggaaatgc ttgccgaact cgaccgccag gggcagtccc
     7141 acatagccga gaccgaccac gccgatacgg cacttacgca ggttcagggg cattcgcttt
     7201 cacttggtgg cggcgacggt tgtgtcgcac gacgcaattt tagaatcaag cacttgaacg
     7261 attcatctcc ggcgtctcgc cagcttcgcg gagccacagg caagccccgc cgctggccgc
     7321 ggagatgatc tgcagcatgg ctgcgtgagc ggccatttcc tcttcactcg cgctgatgat
     7381 caccagcggt atcggtgggc gcaccagcgc acgtacggtg cgcacggtct ccgccgtgcc
     7441 cggcatggcg atcgcatcac tcagcgccag agcgccctga cctccggtca tggcgagata
     7501 tacgtccgcc agaatccgcg catcgagcaa ggcgccgtgc agctcgcgac tggaattgtc
     7561 gacgctgtag cgcttgcaca gagcatccag gctgtttcgc tgcccggggt gcatggaacg
     7621 cgcaagcgcc agcgtatcga gcacgctgca tacgcccgag atcgttcgcg cctcgccctg
     7681 gacgcgcgcg agctctgcgt ccaggaaggc gacatcgaat gcagcgttgt gtatgaccag
     7741 ctccgctccg ccgatgaatt cgatcagccc gtcggcgaca tccatgaagc gcggcttgtc
     7801 gagcagatcc tcgcgcgaaa tgccgtgcac gcccagcgcg ccctcctcga tgtcgcgctc
     7861 cggattcaga tagcggtgga aggtgcgccc cgtgggccgg cggttcacca gctccacgca
     7921 tccgatctca atgatgcggt gaccgagctc gggctcgagt ccggtggtct cggtgtcgag
     7981 gacgatctgg cgcatggtcg cgcagtctac cggagcaggg catcgatggc ttggttcgca
     8041 agctgatcga cccgctcatt tccgggtaca ccggagtgtc cgcgaaccca gtgccattcg
     8101 atctcgtgaa ccgcagccag ctcgtcgagg cgctcccaca gatcctggtt cttcaccggc
     8161 ttgcgatctg ccgtgcgcca gcctcgggct ttccagctgt gaatccactc cttgatgccg
     8221 cggcgcacgt actccgagtc ggtgaacacg ctcgcgcgga ccgggcgctt gagtgcctcg
     8281 agcgcccgga tcaccgcggt gagctccatg cgattgttgg ttgtgagcgc ctcagctccg
     8341 gccagctcct tctcgctgtc ggcaaatcgc agcgtggcgg cccagcctcc cggtcccgga
     8401 ttgccgcggc aggcaccgtc cgtatagatc tccacaacct gcgcggcagg ctgctgcgta
     8461 aggctcacga ttgcgaacgc gtcgtcggct tgacgaggcc acccagcacc gccggctttt
     8521 cgcggaagcg gggtcgcacc ggagtcagcg tatagacacg cttgcgcgcc ttgaggaggt
     8581 aggcgcttcc cgggaagggg taggtaaagc ctcgccgcag aatccttccg acgccctcgc
     8641 ccgctgcgcg ccgcgagctc cagggactgc aatagaggta acgacgggat gcgaccactt
     8701 catagcccag caggacgagc cagtcccgga cgcgcctttc ggagagcacg cgtcgcatgc
     8761 ccggtgggaa gccatcacgg ctggcgcgcg cgcgcatgcc ccacaggccc caggggcaga
     8821 atccgagcac gagcagctgc ccctcgccga ccagtatgcg atcgacctcg cgcacgatcg
     8881 catattggtc ggctgcgaat tccaacgtgt gcggcagcaa taccgcgtca accgaatcac
     8941 tattgatcgg cagcagtgag ggcctgccga tgatgtccgc gccgcaggga aatgcgggcg
     9001 ccgcgacgat cgtctgacga cgggtgcggc tgcaggcgag cagctcccgc gagccgcccc
     9061 atgcgccgat ctgcagcaat tcccagccga aaacgtcttc gagtgcctca ccgagaagct
     9121 cggactccgc ggcgagaagc gcgcgtccga gcgggctagc ccaccagctg ctcagggccg
     9181 catcgcggtc cgggattcgt gaaatgcgaa ctggcacgaa ctcgggctgg caatattgag
     9241 aagttaccgg ttaaattccc tgccagatct gctgtagcgt tgacccgcca cccgcgctcc
     9301 ggcgccgggg accttagcat gcccggaaaa cgagggtaag agacttggcg agggagccga
     9361 ttctgaagac gcgttgggtg ctggtgctgt cagccctcgt attgatttcg ggctgcgcac
     9421 atgcgccgcg ccggcctgtc accccggcgg ctccggccgc cgatgccgtg cctccggtcg
     9481 agccgagcag cacgtatccc gaggccccca cgccgcccgc agagcaggcc cccgcccccg
     9541 aagatgccac gctcgcccag caacccacac aggtcgcggg gctgacgcca gcccagtacg
     9601 cggatctgtt cgatcggatg agagccggct tccacatcga agatgcggac cgcgcgatga
     9661 tcgatcagca gctcgcctgg ttcgcctcac atccggacta tctggagcgt gccttcggac
     9721 gcgcagagct gtacctctac cacatcgtga ccgagctgga agcgcgcggc atgccgctgg
     9781 agattgccct gctgccggtc gtggagagcg cattcgagcc gtacgcttac tcgcgagcac
     9841 gcgcctcggg tctgtggcag ttcatcccgg gtaccggctc gcgctacggt ctgcgacagg
     9901 actggtggta cgacgggcgg cgcgacgtcg tggaatcgac ccgcgcggcg ctggactacc
     9961 tgcagtcgct gcacgatgag ttcaatgggg actggctgct ggcgattgcg gcatacaact
    10021 gcggcgagct cgccgtggag cacgcgctcg atgtcaatcg ccagaagggc aagccgctgg
    10081 acttctggag cctgaagctg ccgaaggaga cgcaggccta cgttcccaag ctgctggcca
    10141 tgcgccgcct ggtgctcgat cctgagaagt atgacatctc gatcagcccc atccccaatc
    10201 agccttactt cactcgtgtc gatacacggg gccagatcga tctgaagctc gccgccgaaa
    10261 tcgctggcgt gaccgatgag gaaatctacg agctcaatcc cgcctatcac cgctgggcga
    10321 ccgatccggc cggaccgcac tacctgctgc tgccgattga cggggcccag acattttccc
    10381 agaacatctc acagctgacc gacgatcagc tgatgcgggc cacgagctat acggttgcca
    10441 agggtgactc ggtgatctcg gttgcgcggc gcttccacac caccccggat gtcatacggg
    10501 agatgaacga gcttccgagc ggtccgctca cggtcggggc ggatctgcgc gttccgtcca
    10561 cctcggtcac cttgccggcc aaggtgctgc ttgcggcggc gcgcgtcgat gggcatgacc
    10621 gctttgccag gcgtccgcac gtgcacgtcg tgcaccgagg cgactcgctc tggagcatcg
    10681 cgcgacgcac ggggatgaat gtcaacaccc ttgccctgat gaatggcatg caacccgggg
    10741 acccgctgcg tgcgggccag cggctgcggc tctcgaatcg cgggactgcg agcacgggtt
    10801 cggcgaaatc ctccagacag gtgacctaca cggtccgcag cggcgacacg ctgacgcaga
    10861 tcgctcgact cttccaggtc tcggtgaagc agatcctcgc ctggaacggc atgatctcac
    10921 gcgctctgac gcccggtcag aagctcacga ttcgcgtctt gagccgaggc ggctgaacgc
    10981 gacgtcctcg ctcgcgccgg gggtcctggc gcatcccacc agcgttcgtt gctacacttg
    11041 ggctcgtcag cagctgcccg accgggcgct gggaggctta cccatgggat ttctcaccgg
    11101 caagcgcgcc ttcatcgtgg gggtggcgac cgatcgctcg atcgcctggg gaattgccca
    11161 ggccctgcac cgggagggag cggagctcgc cttctcgtac gtcaatgaca agatgaagga
    11221 acgcgtggaa cctctggcgc agtccctcgg gtcaacgctg atcatgccgc tcgatgtgac
    11281 gatcgacggc cagctcgact cggcattcga gctgatccgg cgcgaatggg gacagctgga
    11341 catcgtgatc cacgcagtgg cttacgcgcc gcgggaagcc atcatgggca gtttcgtcga
    11401 taacaccagc cgcgaggcgt ttcgcatcgc tcacgatgtc tccagctaca gtctcactgc
    11461 gctggcgcgg ggcgcgaagc ctctcatgtc cgggcgcaag ggcgcgcttc tcacgctgtc
    11521 gtatcttggc gctgtgcgtt cgatcccgag ctacaacgtc atgggtctcg ccaaggcaag
    11581 cctggaagcc aacgtccggt tcctcgccgc ggatctgggt ccggcgggaa tccgcgtcaa
    11641 cgggatctcg gcgggtccta tcaagacgct ggcggccgct ggcatcgccg gctttcgcaa
    11701 gatgctcagc cgtgtcgccg aaatcgcgcc tctgcggcgc aacgtcacgc ccgaggacgt
    11761 cggcaacgct gcggcgttcc tctgctccga tctcgcagcc gccatcaccg gtgagatcct
    11821 gtatgtggac aacggcttca gcacggtcgg catgagcttc tctgagcagg gcaacgactg
    11881 acggaatggc tcggcggatc ttcatcaccg gcgccgccgg gttggtcgga cagaacctga
    11941 ttgcgcacat cgaacaacaa ggtcctgccg agatcgtcgg gctcgacaag cacgcgcaca
    12001 atctggcggt cctggcgcgg ctgcacccgg acgtccgcct catcgagagc gattgcgcga
    12061 cctctggcga atgggaaaag gtgctggggg aatgtgatga gttgatcctg ctgcacgccc
    12121 agatcggagg actgagcgca gcggagttcg agcgcaacaa cgttgcggcg actcggcgtg
    12181 tcctcgaggc tgccgctcgc ggccgggtgt cgcacatcgt tcacgtgagc tcatcggtgg
    12241 tcaactcacg cgcccaggac ctgtatgtac agacgaagaa gcagcaggaa gcgctcgtat
    12301 cggcctcggc gatcccccac gtcattctgc ggcccacgct gatgttcggc tggttcgacc
    12361 gtaaacacat gggatggctc atgcgcttcc tgcggcgcgc cccgctgttc ccgattcccg
    12421 gcagtggcaa atatctgcgc cagccgctgt atgccggcga tttctgcgcg atcgtgctcg
    12481 cctgcatgca acggggcatg gtgggtgagt acaacatctc gggattgtcc cggatcgact
    12541 acatcgatct catccgcgag ctgcgccggg tatcgggcgc ccgcaccccg atcgtgcgcc
    12601 tgccctactc ggtcttccgg ctgttgctcg cgacctacgc actgttcgat cgcgacccgc
    12661 cgttcaccac cgcgcagctg cgcgcactgg tcatcccgga gctcttcgag gtcatcgact
    12721 ggcccgcaat ctttcaggtc acaccgactc cactgcacga ggcgctggag cgcaccttca
    12781 acgatcctcg cttcgccgat gtcgcacttc gattctgagc cgcacgcgcg cgatctgctg
    12841 gtcattggcg ccgggccgat gggcctcgct gccgcttact ttgcctgcgc gcgcggctat
    12901 cgggtcgaag tgctcgaggc cggtgaccgc cccggcggga tggctgccca tttcgatttt
    12961 ggcggtctgt cgctcgagcg cttctatcat ttttgctgca agaccgacgt ggatacgttc
    13021 gcgctgctgt ccgagctcgg cctgccggcg gccgtgcact gggtcccgac ccgcatgggc
    13081 tacttcgtcg ccggccgatt gcatcccttt ggcgatcccg tggcgttgct gcgcttcccc
    13141 tggctcagcc tcgtcgagaa aatgcgctac gccgccatgg cgttctgcgc cacgaaacgc
    13201 agcgactggt cggcgctaga tcgcctgagc gcccgcgatt ggctcgtcag atggtgtggc
    13261 ccacgcgcct atgaccggat gtggcggccg ttgttcgagc tgaagttctt cgagttcgcg
    13321 aactcgatct cggcggcctg ggcctggcaa cggatcaagc gcctcggcaa gtcgcgccgc
    13381 tcgctgttcc aggaagagct cggctacatc gagggcggct ccgaggcgct catcgagcgg
    13441 ctggtcgatg ccatcgagcg aggcggcgga cgcattcgcc tcaaggagcc cgtgcgccac
    13501 atcctcgtgg aggacggcac ggtgaaaggt gtcgagaccg ctgcagcgca gagggcgccg
    13561 gcggctgcgg tcatctcgac cgtgcccctg ccctacgtcc ccgccatgct ccagcgcgat
    13621 gccccgcagc ttgccgcgcg atacacgggc ctggataaca ttgccgtcgt gtgtgtggtg
    13681 cacaaattgc gacgctcggt gtccccacac ttctgggtca acatctgcga cccggacatc
    13741 ggcattcccg gtttcgtcga gttttcgcac ctgcgtccgc ttccggacac catcgtctac
    13801 gttccgtact acatgcccgc cactcgcgag cgctttagct ggtccgatga ggcactcggc
    13861 gatgagagtt tccgctttct gcagcgcatc aacccggccc tgaccgccgc cgaccggatc
    13921 gacacgcacg tggcgcggct gcgctacgcc cagcctgtct gtacggttgg cttcgcatcg
    13981 aagctgccgc ctgcgcaaac cagcatcgcg ggcctgcaga tcgcagacac gtccttctac
    14041 tatccggagg atcggggcat ctcggagagc atcaaattcg cgcgccgcat ggtgcaggct
    14101 ctgccgagcc acgcgaaagc cggctgagcg ctcagcgcac ggccaccgct tcgccgcgaa
    14161 gctatcccgc cacgaattgc ggcggcagcc tcagcgcgcc gcggagggct ttctcgccac
    14221 gatcagaaat tgcttcccca ggaaccggga gctcagtggg cgcagtcgca gatacagcgc
    14281 caccagccag gcagcctgtg gcaatgcgga gcgggtcgtg tagggcagga accgcggctc
    14341 gacccggacg agatcaaacc cggtcacgct caaaacctcg caaaggctgc ggtcgctgag
    14401 ggggacgtga tgatcgaaga agtcccagta gggtccgggg atcagtcgca cgttcggacc
    14461 cagcaggatc agcgtgccgc cgggccgcag cactcgcatc gactcgctca atgcgctcgc
    14521 gacatcctgc ttgcaggaca gatgctcgag gaagttcgac gagaacacga catccaccgc
    14581 acccggcccg agaaatccca gctcggtcgc cgagcaggcc tgcacttcga cgcccggcgc
    14641 ggcgagccgc gcggtgtcgg gattgagatc gaccgcgatg cgtcgcccgg cgcgggcgga
    14701 gttgatgaac tcgcagaagc cggcgcccag atcgagcacc gtgtcagtcg gccggatcca
    14761 ctgttgaaac acgtgccggt agagcacatc ccagacgact gccttgcgtg cgcgggccgt
    14821 ctcatcgaac ctgttcaggt acagctgctg aagatccatg cgggttctca cctgaaggtg
    14881 aagaggcgat ggccgaagta gctggtgagc atgggcacgg tgactcctgc caggtggccg
    14941 agtgcctgcg catgcgcttg caatcccacc gcgggcagcg cccagcgggc gagcgccacg
    15001 ctgatcgcca gcgtttgtgc caaagcgagc agattcacga ccacgaaccg tgccgcttgc
    15061 gggccgatgg ctttaccctg cccgccgaac acatgtgcgc gcatcagcac aaagccggtg
    15121 gccagaccga cgacgaaggc gagaacgacg gcccactcat agcgaaccca gagactgaag
    15181 atgaaacgcg agccgaagtt cgccgccgcc gccacgcctc cggccagcag gaaattgcgg
    15241 aactgtcgcc gcagcggctg cgaatccgcg cgcgcatcgc cgacagcggg ggcactcgtg
    15301 gggccggcgg atgcagcccc cgggctgcgg gccagcggcg gcgtgctcat agcttcagcc
    15361 gcttgaagat cgtttcgggc agcaggcgaa tgaccgtcat gatcagacgc cagaaacccg
    15421 gtacataaca gacatcccag cgccgatcga tggcgcggac gatgcgccga gctacggcgg
    15481 cgggctcggc ggtcaacagg cgaggcaggt tcagatgagc cgtcatgctc gtggcgacga
    15541 accccggttt gatcgtcagg acgtgcaccc gctgagccca gagctgcgcg cgcaatcccg
    15601 atgagaaggc gtgcacggcc gatttcgcgg cgccgtacag atagtttcca ggccgcccgc
    15661 gatcgccggc caccgaaccc acgaccgcga tgataccgcc cccctgcgat tggaagcgct
    15721 gcgcaaggcc ggcgaggcag agcagaacac tttcggcatt gacccgccat tcacgcacca
    15781 ggtaagtact gtccactgcg gcacgcgcct ggtcgggcaa tgagccgtaa gcgatcagcg
    15841 cgacgtccac ctttccgagc gccgcccagg ccgattgctc catggcctgg atctgcgcgg
    15901 cgtcctccgc gtccatgatg aaaatgtcga ctgcgcccgc gccccgggct cgcaagtcag
    15961 ccgccacggc ctcgagtctc tgtgggttgc gggcggcaag gaagagccgc gacccgcgtg
    16021 cggcatagca gcgacccacc gcctgcgcaa ttccggaggt cgcgcctacg atgaccacgt
    16081 tctgcgtcat tgcgtcacgc gctgccagaa ccgggacatc agccgcggat cccgcagcgc
    16141 ctcgacgctg cgccaggcgg ggtaggcact ctgaaaatgc ccggccgtca tgtgagcatc
    16201 cttggccgga tagagtcggc caccggcatc atgaaccaac gcatcgagct cgccgaacaa
    16261 agcgacggtg cgcgcgctct gcgggaagtc cagcgcgagc gagactcccg gcatcgggaa
    16321 tgacaacagc ccgggcgaag tcagagcccc gcatttcttc aggacggcca ggaatgaccc
    16381 ggtgccgctc ctcgcgatcg cgcgcaccac cgagcggatg ctctcgcggc tggtcgcatc
    16441 cggcacgatg cactgatact gctgaaagcc cgcacgtccg tacatgcgat tccagccgga
    16501 aatgcgatcg agcggataga agaacgagtc gtagcctgcc gagcgctgca cctctctgcg
    16561 cagctggctg cggtagtaga cggcattgaa ggcacgcagg gtgaagggat tgaccaggga
    16621 gaacggcggg tcgatcggga ccttcagggc gcccggctgc tcacatctca aggcaccgtg
    16681 gggagcgtga ttgccaacaa tgagatgccc gcgtccggca gttttgccgg acgcggcaca
    16741 gtcgatccac gcgaccgtgt actcgtagtc ctggtcgtac ccggcaacga gatcgaaaaa
    16801 ctcatccagt ccgcgaaagc ggatgcgccg ctcctgaatg cgcgcggagc tcaccgggcg
    16861 cagttgcaat tccacactca ggatgacgcc ggtcaggccc aatcccgcga tggtcgcggc
    16921 gaacagctcc ggcgcctgcg tgcgcgatat ggtgctcgcg cccgtgtcgg tccgatacag
    16981 cgtgaggctt ccaacatggc ggccaaaggt gcccatgacg tgatgattct tgccgtgcac
    17041 gtcgttggcc acggccccgc ctagcgtgat gaacttggtt cccggggtga ccggagggaa
    17101 ccaaccccgc ggcagggcca cttcgatcaa ctcggccagg gtgaggcccg cttcagctac
    17161 gatccggcca ctctgccagt ccgccgccag gatgcggttc atgttggtca tcatcagcac
    17221 gtgatccgat accgcgaggc atgcatcccc ata
//
LOCUS       DASRHN010000118         6994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_530985, whole genome shotgun sequence.
ACCESSION   DASRHN010000118 DASRHN010000000
VERSION     DASRHN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530985"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFB37_04475"
     CDS             complement(<1..949)
                     /locus_tag="VFB37_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO21738.1"
                     /translation="MGLAQLRRLGARGSELTQWALPCDLPALLGPLADLLVFIGLTQG
                     GLGLGPAHILSFVAAAMLNYLVNVRRAVRGAGRSGDLRLYGYLFTISAFALFLRGGVL
                     SLFAVTWGWPPAAAMVFAALTSAIVTQWGYGFALSSAVWNVGRGSRWQLLAWSVVVCA
                     FVLRLVYLAQIELLPEETYYWNYSRHLDIGYLDHPPMVAWLIRLGTTICGDTQFGVRI
                     GALGCALCASFFAYRLTRNLFGEASALIALVLMQVLPFFFLAGVLMTPDAPLTAGWAA
                     SLYFLERAILGGRSNAWWLTGLSVGLGLLSKYTISLLVPA"
     gene            complement(1107..3362)
                     /locus_tag="VFB37_04480"
     CDS             complement(1107..3362)
                     /locus_tag="VFB37_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZO21739.1"
                     /translation="MKALLFALLLVLPLSWASAQDLEFHPPASAADPAAAAVMRDLAT
                     RILPVYQENDQDRYLKNLSALQLVARNFTGAWATRQSLRDRRKSSEARKPLSSSIIYD
                     MYVQARDVEAEKGTPFDRGFAQAYRDTVSKLSDQDAYNVTGWLALPVSGFQEVVQRTF
                     DQWRSKGSIPQVDAVDLIWNYLVFDAYRSFHPLIAALNEEDDGRRYVKDYDVLIDTPE
                     HESISAIMVRPKDDTKPLPTLLEFTIYVDARTFARECAAHGYVGIVAFTRGERRSRGK
                     VLPYEHDGEDARAVIDWIAKQNWSDGRVGMYGGTYSGFAAWAALARHPPAALKAIAAT
                     SATAPGIDLPMLNNIVRNSAYRWSGCVSGEKDFDDKTCADDARWRQLDEDWYRSGKSY
                     RDLDRWLGGHNVIFHTWLDHPSYDRYWQKLIPFREGFAHINIPVLQTAGYYALGEAGT
                     LYYFTEHYKYDPHANHTLLIGPYDDGSMQHGAPVNLQGYQVDQAALVDLRELRYQWFD
                     SIFKGAEKPALLQGRVNYEVMGSNEWRHVASLEAMGTGTMRLYLDAGAGAEYGHRLLK
                     RKPAKPTYVQQVVDFKDRSDADWTAPTGVQSHSLQLHDAIAFVSEPLQAAAELGGLFS
                     GKLDFTVNKMDLDLNMTLYELLPSGEYLKLFEPAYEFRASYVHDPVNRRLLHAGERQH
                     LTFRSDRLMSRKLEPGTRLLVVLGVNKRADRQINYGTGAPVNDENIDDARIPLKVRWY
                     SDSYIDLPVAK"
     gene            complement(3359..5179)
                     /locus_tag="VFB37_04485"
     CDS             complement(3359..5179)
                     /locus_tag="VFB37_04485"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZO21740.1"
                     /translation="MTGAAEPNPETAPEVESSPHSGLRRQLGMIMRALGRSTVVKTLV
                     FMVLAIIIIVVVTAYCQIRLNRWNKPFYDALSRRDLHDFLTQLAVFFVIAGALLVLNV
                     VQRWLVEMLKVRMREGLVQDLLRDWMLPRRAFWLANAGGPMGVNPDQRMHEDARKLCE
                     LSADLGTGLLQAGILFGTFAGVLWVLSSDFSFRFGDRDYAVPGFMVWAAIVYAGAGSL
                     LSYWVGRSLISRNAERYAREADLRFSLVRINEHLDGISLASGEEDERRRVELHLGNVL
                     AATRRLVTGLTNLTWVTAGFGWITLVAPILVAAPLYFAGKISFGGLMMAAAAFTQAQS
                     SLRWFVDNFSTIADWRATLHRVASFRHALSGTEVLQDFESRINYAEGPAGQMTIEALE
                     IASPVGYDMLREREIVVKAGERILIIGAPGTGKTLLFRALAGLWPWGAGSVTHPKDDL
                     MLYLPRGTPYLPRGTLREVLAYPHHVDRFNEGAFRHALERLGLQRLAPMLDATRRWDR
                     ELSQDEQLALAFARIALQAPPWLLIDDMLGSLDDEALERVIDLFAHELAHSSLIHIGR
                     AAQARDPLFSRVLHLVRAPPRPPQHHNQAAQAQGAPADHR"
     gene            complement(5210..6484)
                     /locus_tag="VFB37_04490"
     CDS             complement(5210..6484)
                     /locus_tag="VFB37_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZO21741.1"
                     /translation="MSISAVSLEKRRPGFGQTVRPHRGSIVASPVLADWDPEPDYFFH
                     WFRDSAVVIEALRLLLEAGHVGPEALGHFSDFVDFSLSLRSLDGGTLLAERSWRERCS
                     PQFEKFLRSDSELGAVKGEKVVAEARVNPDATLDFSSWTRPQHDGAPMRALVALRWAH
                     AVPLPRDLKLRVAELVRIDLAFTERHWREPAYDIWEEEKGLHYYTLCVSAAALQEGAR
                     WLEEEDEPALAHRYRLEVSSIHSRLDGFWLSESGHYRSRVLDAGARSSKELDISVILA
                     AIHAAGEGAMHTVDDPRMHDTLDRLEALFDRTYAINQARAAHAGPAMGRYEGDVYFSG
                     GAYYFSTLGAAEFCFRAALHSDKAAAWMERGDAFLQTVRAYTPASGEMSEQFDQRTGA
                     QSSARHLAWSYACFITCIAARRAALGRALIRS"
     gene            complement(6556..>6994)
                     /locus_tag="VFB37_04495"
     CDS             complement(6556..>6994)
                     /locus_tag="VFB37_04495"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZO21742.1"
                     /translation="NHAHCVANPSAHKGYGESCWGLTASDDPDGYSVHAPDLDNGTIA
                     PTAAVASLPYAPSEGLSAIRHFLGHRGGRLWGRYGFVDAFCEQRNWYADTYVAIDQGP
                     SVIMIENHRTGLLWKLFMSVPEVQVGLRRLGFSSPQLARPTAR"
BASE COUNT         1338 a         2256 c         2268 g         1132 t
ORIGIN      
        1 ttgccggaac gagcaggctg atcgtgtatt tcgacaacaa gcccagaccg acgctcaggc
       61 cggtgagcca ccacgcgttg gagcgtccgc ccagaatggc gcgttcgagg aaatacaacg
      121 aggcagccca gcccgctgtc agcggcgcat cgggtgtcat gagcacgccg gcaagaaaga
      181 agaaaggcag cacctgcatc agtaccaggg cgatgagcgc actggcctcg ccgaacaggt
      241 tgcgtgtcag ccggtacgcg aaaaacgagg cgcacaaggc gcagccgagt gcgccgatgc
      301 gcacgccgaa ctgtgtatcg ccgcaaatcg tggtccccag acggatcagc caggcgacca
      361 tgggcggatg gtcgaggtaa ccgatatcca gatgccggga gtaattccag tagtaggtct
      421 cctcggggag caactcgatc tgggccagat agacgagtcg caacacgaaa gcacagacca
      481 ccacgctcca cgcaagcagc tgccagcggg agcctcggcc gacattccag acggctgacg
      541 agagcgcgaa gccgtagccc cactgggtca cgatggccga agtaagcgcc gcaaagacca
      601 tggcggctgc agggggccag ccccacgtca ccgcgaacaa ggacagtacg ccaccgcgca
      661 gaaacagcgc gaaggcactt atcgtgaaga gataaccgta caaccgcagg tcgcccgagc
      721 gcccggcgcc cctcacggca cgtcgcacgt tgacgaggta attcagcatt gcggcggcca
      781 caaagctcag gatatgcgcc ggcccgagac ccaaaccgcc ttgcgtcagt ccgatgaata
      841 cgaggaggtc ggccagcggt cccaggagtg ccggcagatc gcagggaagc gcccattggg
      901 tcagttcgct gccgcgggcg cccaggcggc gcagctgggc cagacccacc tgccatctga
      961 gcggttgagt tcctgaagac ggcacgttgg gttctggggt gcgggatcgt cgagtggctc
     1021 agtagacaat catggaccgc cgcggcaagg cagagcaacc cgagcgggca ccaaggatgc
     1081 tcgccgggct cgcacgccac agctgcttac ttggccacgg gcaggtcgat gtagctgtcg
     1141 ctgtaccagc ggaccttcag cggaatccgc gcatcgtcga tgttctcatc gttgaccggc
     1201 gcgccggtac cgtagttgat ctgccggtcg gcgcgcttgt tgacacccag caccaccagc
     1261 agccgcgtgc ccggctcgag cttgcgactc atcagtcgat cgctgcggaa ggtgaggtgc
     1321 tggcgctcac cggcatgcag caggcggcgg ttcacggggt cgtgcacgta gctggcgcga
     1381 aactcgtagg cgggctcgaa cagcttcaga tactccccgc tcggcaacag ctcgtacagc
     1441 gtcatgttca ggtccaggtc catcttgttg accgtgaaat cgagcttgcc ggaaaacagg
     1501 ccgccgagct ccgcggcggc ctgcaacggc tcgctcacga atgcgatcgc gtcgtgaagc
     1561 tgcaagctat gactctggac ccccgtcggc gccgtccagt cggcatcgct gcgatccttg
     1621 aaatccacga cctgttgcac gtaagtcggc ttggcaggct tgcgcttcag caggcgatgt
     1681 ccgtactccg cacccgcacc ggcatcgaga tagagccgca tggtgccggt ccccatcgcc
     1741 tccagtgagg ccacgtggcg ccactcgttc gatcccatta cctcgtaatt gacgcgaccc
     1801 tgcagcaatg caggcttctc ggcgcccttg aaaatggagt cgaaccactg ataacgcagc
     1861 tcccgcagat caacgagagc cgcctgatcc acctggtagc cctgcagatt caccggcgcg
     1921 ccatgctgca tcgagccgtc atcgtagggt ccgatgagaa gcgtgtgatt ggcgtgagga
     1981 tcgtacttgt agtgctcagt gaagtaataa agcgtaccgg cctctccgag agcgtaatag
     2041 ccggcggtct ggagcaccgg gatgttgatg tgcgcgaaac cctcccggaa gggtatgagc
     2101 ttctgccagt agcggtcgta gctcggatga tcgagccagg tgtgaaagat cacgttgtgg
     2161 ccacccagcc agcgatccag gtcgcggtag gacttgccgc tgcggtacca gtcctcatcg
     2221 agctgacgcc agcgggcatc gtccgcgcac gtcttgtcat cgaagtcctt ctcgcccgag
     2281 acgcagccgg accagcggta cgccgagttg cggacgatat tattgagcat cggcagatcg
     2341 atgcccggag cggtcgcgga agtcgcggca atggccttca gggcggccgg tggatgcctg
     2401 gcgagggcgg cccaggctgc aaagccgctg tacgttccgc cgtacatgcc gacccggccg
     2461 tcgctccagt tttgcttcgc gatccaatcg atgaccgcgc gggcatcctc gccatcgtgc
     2521 tcatagggaa gcaccttgcc gcggctccgg cgctcaccgc gcgtgaaggc aacgatgcct
     2581 acataaccgt gcgcagcgca ctcgcgggcg aaggtgcgcg cgtccacgta gatcgtgaat
     2641 tccaggagcg tcggcagagg cttcgtgtcg tctttcggcc gtaccatgat tgccgagatg
     2701 ctctcatgct cgggcgtgtc gatcaggaca tcgtagtcct tgacgtagcg ccggccgtcg
     2761 tcctcctcgt tgagcgccgc gatcaacggg tgaaagctcc gataggcatc gaacacgaga
     2821 tagttccaga tcagatcgac ggcatcgacc tgaggaatgc tgcccttgga acgccactga
     2881 tcgaacgtac gctgcacgac ctcctggaag ccgctcaccg gcaatgccag ccagcctgtc
     2941 acgttataag catcctgatc gctgagcttc gagaccgtgt cccggtacgc ctgggcaaag
     3001 ccgcggtcga atggcgtgcc cttttcagcc tccacatcac gtgcctgcac gtacatgtcg
     3061 tagatgatcg agctcgagag gggtttgcgg gcttcgctgc tcttgcggcg gtcgcgcagc
     3121 gactgccgcg ttgcccaggc acccgtgaag ttgcgggcca cgagctgcag agccgacagg
     3181 ttcttcaggt accggtcctg atcgttctcc tgatagaccg gcaggatgcg cgtggcaagg
     3241 tcccgcatga ccgcggcggc cgctggatcg gccgccgaag cgggagggtg aaactccaga
     3301 tcctgggcgg aggcccagga gagcggtagc acgagcagca gcgcgaagag cagcgccttc
     3361 atcgatgatc ggccggcgcg ccctgcgcct gggcagcttg gttgtgatgc tggggcggcc
     3421 gcgggggtgc ccgcaccaga tgcaggacac gcgagaacag cgggtcgcgc gcctgggccg
     3481 cccgcccgat gtgaatgaga ctcgagtgcg ccagctcgtg tgcgaaaagg tcgatcacgc
     3541 gctcgagcgc ctcatcatcg agcgatccga gcatgtcatc gatcagcaac caggggggcg
     3601 cctgcagcgc gatgcgcgcg aacgcgagcg cgagctgctc gtcctgactg agctcgcgat
     3661 cccatctgcg tgtcgcatcc agcatcgggg ccagccgctg caggccgagg cgctcgagcg
     3721 catgccgaaa cgcgccctcg ttgaaacgat ccacgtggtg cggataggcc agcacctcgc
     3781 gcagggtgcc gcgcggcaga tacggggtgc cgcgcggcag gtacagcatg aggtcgtctt
     3841 tgggatgggt gacgctgccg gcgccccacg gccacaagcc ggcgagcgcg cggaacaaca
     3901 gtgtcttgcc cgtaccaggc gcgccgatga tcaggattcg ctcgccggcc ttcactacga
     3961 tttcgcgctc gcgcagcatg tcatatccga caggcgatgc gatctccagc gcctcgatcg
     4021 tcatctgtcc cgcggggccc tcggcgtagt tgatccggct ctcgaaatcc tgcagcacct
     4081 cggtgccgct gagtgcatga cggaagctgg cgacgcgatg cagggtcgcg cgccagtcgg
     4141 caatcgtgct gaagttgtcc acgaaccagc gcagggagga ttgagcctga gtgaaggccg
     4201 ccgccgccat catcaggccg ccgaaggaga tcttgccggc gaaatacagc ggcgcagcga
     4261 ccaggatcgg ggcgaccagc gtgatccaac cgaagcccgc agtgacccat gtcaggttgg
     4321 tgagcccggt gacaaggcgt cgcgtcgcgg cgagtacgtt acccagatgc agctcgacgc
     4381 ggcgccgctc gtcctcctcg cccgaggcaa gcgagatgcc atccaggtgc tcgttgatgc
     4441 gcaccaggga gaagcgcaga tcggcctcgc gcgcgtaacg ctcggcgtta cggctgatga
     4501 ggctgcggcc gacccagtag ctcaacagag agcccgctcc ggcgtacacg atggcggccc
     4561 agaccatgaa gcccggcacg gcatagtcgc gatcgccgaa gcggaagctg aagtcgctgg
     4621 agagcaccca gagcacccct gcgaaggtgc cgaacaggat cccggcctgc agcagacccg
     4681 taccgagatc ggcggacagc tcgcagagct tgcgggcgtc ctcgtgcatg cgctggtccg
     4741 gattgactcc catggggccg cctgcgttgg cgagccagaa tgcgcggcgg ggcagcatcc
     4801 agtccctgag caggtcctgc accaggccct cgcgcatgcg caccttgagc atctccacca
     4861 gccagcgctg caccacgttg agcaccagga gcgccccggc tatcacgaag aacactgcga
     4921 gttgcgtcag gaagtcgtgc aggtcgcgtc gcgagagcgc gtcgtagaag ggcttgttcc
     4981 agcgattgag gcggatctgg cagtaagcgg tcacgaccac gatgatgatg atcgccagca
     5041 ccatgaagac gagtgtcttc acgaccgtgg agcggcccag cgcccgcatg atcatgccga
     5101 gctggcgacg cagacccgag tgcggagacg attcgacctc cggcgccgtc tctggattcg
     5161 gttccgcggc gcccgtcatc gtgcctggtc cttgccctgc ctcaccgggt cagctccgaa
     5221 tcagggcccg gccgagagct gcgcgccgcg cggcgatgca ggtaatgaaa catgcgtagc
     5281 tccacgcgag atgtcgcgcc gagctctgcg caccagtgcg ctggtcgaac tgttccgaca
     5341 tctccccgct ggcaggtgta taggcgcgca cggtctgcag gaaggcatcg cccctttcca
     5401 tccacgctgc cgccttatcg gagtgcaacg ctgcgcggaa gcagaattcg gccgccccga
     5461 gagttgaaaa gtaataggca ccacccgaga aatagacatc gccctcatac ctgcccatgg
     5521 ccggtcctgc gtgagccgcg cgcgcctgat tgatggcata cgttcgatcg aacagggctt
     5581 cgagcctgtc cagcgtgtcg tgcatccgcg gatcatcgac ggtgtgcatt gcgccctcgc
     5641 ccgcggcatg aatcgccgcc agaatgaccg aaatgtcgag ctccttggag gaacgcgccc
     5701 ccgcatcgag cacacgcgag cgatagtggc ccgactcgga cagccagaac ccatcgagcc
     5761 tgctgtggat actcgagacc tcgagtctat agcgatgcgc gagcgccggc tcatcttcct
     5821 cctccagcca gcgggcacct tcctgcagag ctgcggcgga gacgcacagg gtgtagtaat
     5881 gcagcccctt ctcctcctcc cagatgtcgt acgctggctc gcgccaatgg cgctcggtga
     5941 aggcgagatc aatgcgcacg agctcggcga cccgcagctt caagtcgcgc ggcagcggca
     6001 ctgcgtgtgc ccaacgcagc gcgacgagcg ctcgcatcgg agcgccatcg tgttggggcc
     6061 gcgtccagct cgagaagtcg agcgtggcat ccggattgac gcgcgcctcc gcaacgactt
     6121 tctctccttt caccgcgccg agctccgaat cgctgcgcag aaacttctcg aactgcggcg
     6181 agcaacgctc acgccaggaa cgctcggcaa gcagtgtgcc cccatcgagc gagcgcagtg
     6241 agaggctgaa gtccacgaag tcggaaaagt gcccgagcgc ctcgggcccc acgtgtccgg
     6301 cttcgaggag caggcgtaat gcctcgatga ccacggccga atcgcgaaac cagtgaaaga
     6361 agtagtccgg ctccggatcc cagtctgcga gcacgggcga ggccacgatg gagcccctgt
     6421 gcgggcggac ggtctggccg aagcccggac gccgcttctc gaggctgacc gccgagatgc
     6481 tcatcagcat ggcactcgcg gcgcgccggt gctgagcgtc gagccagtgc ccgaaggttg
     6541 gatcctcgcg ctgcatcagc gagcggtcgg ccgggcgagc tgagggctgc tgaagccgag
     6601 tcggcgcaat cccacctgaa cctccggcac gctcatgaac agcttccaga gcaggccggt
     6661 ccgatgattt tcgatcatga tcacactcgg gccctgatcg atggcgacgt aggtatcggc
     6721 gtaccagttg cgctgctcac agaaagcatc gacgaagccg tagcgtcccc agagccggcc
     6781 gccacgatgg ccgaggaaat ggcgaatggc gctcagcccc tcgctcggtg catacggcag
     6841 gctcgcgacg gcggcggtcg gcgcgatggt gccgttatcc agatccggcg cgtggaccga
     6901 atagccgtcg ggatcatcgc tcgccgtgag tccccaacac gactcaccgt agcccttgtg
     6961 agcgctggga ttggcgacgc agtgcgcatg attg
//
LOCUS       DASRHN010000119        17241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_368787, whole genome shotgun sequence.
ACCESSION   DASRHN010000119 DASRHN010000000
VERSION     DASRHN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368787"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFB37_04500"
     CDS             complement(<1..227)
                     /locus_tag="VFB37_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21743.1"
                     /translation="MSKKEVPWREKPAAEDYKAALAYLSLLMSHPEAQKVIRALRSAR
                     TQAWIAKDLLRASRLPLSSSDDRHVSSDLKRL"
     gene            complement(516..815)
                     /locus_tag="VFB37_04505"
     CDS             complement(516..815)
                     /locus_tag="VFB37_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21744.1"
                     /translation="MEALLMPLPAAIAVEARELAELPGRNGMEYDERRQCAYWFTHQP
                     GGMFVCVTFRYVPDLDAVAQLWTVLTDEDSEPSVERLLAVYRQATGIDVDALPLQ"
     gene            complement(1160..1579)
                     /locus_tag="VFB37_04510"
     CDS             complement(1160..1579)
                     /locus_tag="VFB37_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZO21745.1"
                     /translation="MHNITQILKIHPHRRGLLEITDEVQGWVRSQKISAGLLTLFCRH
                     TSASLLIQENAAREVRTDLEAFFETLAPEQPGRYRHEDEGPDDMPAHLRTALTGVQLS
                     VPVIDGKLALGTWQGIYLFEHRNRPHAREIALHLIGQ"
     gene            complement(1555..2838)
                     /locus_tag="VFB37_04515"
     CDS             complement(1555..2838)
                     /locus_tag="VFB37_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZO21746.1"
                     /translation="MIRGLAAALATRMARVKPSAIRELLRLGADPQIISFGGGYPDPA
                     LFPLEKLSAVFEQAIIGQGRSALQYTLSTGMPRLREQIAERMARQGVRCSAENVLILQ
                     GAQQGLDLVAKMLVDRGDLIITENPTFLGALIAFNPYEPGYLAIRMDREGMDTEHLEE
                     ALTEHPRVKLLYTMPDFQNPTGVSLSLARRRRLIELANRFDLIVLEDTPYREIRFEGE
                     PIPTLKSLDTEGRVIYLGSFSKILAPGLRLGWVVAEPELIHELGLLKLAADTQCSTLS
                     MAATSLFLERYDVEAHIEIIRETYRRKKEAMLAAIRGSFPRAVSCTNPHGGMFTWLTF
                     PAGFDTTQFLANEALPKVKVAYVPGATFFPVTEEINHARMSYTTQDHGTITRGVTALG
                     KLVSSFLGDEPECVAAVAEGSRGKTLLQCTTLPRS"
     gene            complement(2835..3593)
                     /locus_tag="VFB37_04520"
     CDS             complement(2835..3593)
                     /locus_tag="VFB37_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO21747.1"
                     /translation="MPFSAIEDRRSVPDRSSNLGEQAYQRIRSEILFYQLPPGSRLSE
                     ASLTSRFGLRQAAVRSALLRLAQEGLIEKTDERSPRVAPLTLKDVRDIYGMRILLEPR
                     AAELGAQLGLAAPDLQRLRELAQSRYEFTNHGELVEFLKANREFNLLIAAGSGNARLA
                     ATITHLQDLTLRILYVGIRSLNVSEWFQTTHLQIADAIAIRDGARAAELWKTDLRYGE
                     RLISDALMRLPELSGVNLAGASLAAAYVSWSKKE"
     gene            3839..4234
                     /locus_tag="VFB37_04525"
     CDS             3839..4234
                     /locus_tag="VFB37_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZO21748.1"
                     /translation="MGARREPIEYEAASPEVRAVYDDIMATRKIDWVNNFWKVLAHDP
                     VALRRIWTNIKQVMGPGALDPLTKELLYVAVSASNGCRYCIASHGAAARKKGMTEAQY
                     SELLAIVGLANETNTLVTALDVAVDPQFR"
     gene            4289..4669
                     /locus_tag="VFB37_04530"
     CDS             4289..4669
                     /locus_tag="VFB37_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO21749.1"
                     /translation="MGAVDFRSVSHHRWDRIPEETLKGTITRRLITGERMMIAHVYLK
                     KGDDVPRHSHDNEQLTYILQGALHFWLGANDERELTVRAGEVLVIPTNVPHRALAMED
                     TLDVDVFSPPRQDWLSGTDAYLRK"
     gene            4671..5405
                     /locus_tag="VFB37_04535"
     CDS             4671..5405
                     /locus_tag="VFB37_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO21750.1"
                     /translation="MNLGLQGRRALVMGGSSGLGRAVAEALVAEGARVAICARNAARV
                     ADTATALGVEGLVGDLSVGGAATNLVREAESRIGNLDVLVVNTGGPPPAPFSALTDAT
                     WREAFEGLWMSSVGAIRSALAGMRTRRWGRIIVITSVAAREPVPNLMLSNSLRAGLHG
                     LVNALSREVAADQVTVNALMPGYTLTERLADLKLDMAALTAQIPARRLGGPRELAALA
                     AFLASEQAGYVTGQAIACDGGLLQSI"
     gene            5439..7853
                     /locus_tag="VFB37_04540"
     CDS             5439..7853
                     /locus_tag="VFB37_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO21751.1"
                     /translation="MKKYGCPKSHLKGFVLVLLAWNAPALWHEAVAQQATATEESASG
                     LQEVIVTAQKRAQNSQDIGIALSAVSGDQLEALGAVTASDITKTMPAVVLTQPNGPSS
                     FSLSIRGVTQNDFADHQESPAAIYVDDVYVSQMAGLAFTMFDMDRVEVLRGPQGTLFG
                     RNATGGLANFVTRRPTDDYSGFADVTFGSHNLTHIEGAVNGQLWHDVDARFSFQSNHY
                     DPLFHNISGGAPDAENGNDWSLRGQLLFKDIGGGSVLFIARGSREDVHAGSWEEYATN
                     PGPNGYDTLLAPNQNAYGTCNGCNASGLTNSGPFVTRDNQAGYARLKTEGLTGKYTAD
                     LSGATLTLIADYSGLRKDYQEDSDASPFTVFQFFNGSNVNQESLEARLNGGDQKLNWT
                     AGLYGLRIDGKYYEGWEGPAFFGAQEFNTPSNPNAAYAAFDGAAGPWPYGAFPFYTGA
                     PAAPNGALPATRAPYSLLTKSYAAFGQVEYRMTELLGFTLGARFTKDQKDYDYTWYPY
                     EYFPQSLTGETVQLTRPSSALLNVYDASRSDNLWSGKAQVDFHLTQDFLAYVSYNRGV
                     KGGGFSAPLFPATINDLATLTFRPETLISYEVGFKSEYLNHTLRINGAAYYYDYHDYQ
                     ALIYTLGLEQLILNADATHKGTEAEIEWAPTEAWDLGLGISYVDAMVNNIPGRCCTAQ
                     GAPVYGNFVPGNAPRWSGNAMIRYTLPLGPGHVALQMDGNYLSRFWFNLGDAPAVEQP
                     AFGIANARVNYTPSGGKIEIGVAVENLANKHYGVMGFDNTSINGLAQVYPGMPRWYKA
                     HINYKF"
     gene            7876..8691
                     /locus_tag="VFB37_04545"
     CDS             7876..8691
                     /locus_tag="VFB37_04545"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO21752.1"
                     /translation="MRTIATAASGTVLAVLYSVCATAAPAAPPSLDEIYATLATKRFV
                     DLTHAFGPSTPHWKGFGDETVKTLYTIKKDGFKVQQFTHVGQWGTHIDPPAHFHEGLR
                     TVDQIPPQEMILPLVVLDVHEKAAKNPDYTIKVADVSAWEAKHGRIPEHAFVAMRTDW
                     SKRWPDDAAMQNRDSAGVAHYPGWSKEVLQLLYEDRKITASGHETTDTDPGLATTHDD
                     YSLERYLLGTNHYQIELLANLDQVPEAGALVVVTWPKPEGGTGFPARVFAILP"
     gene            8725..10092
                     /locus_tag="VFB37_04550"
     CDS             8725..10092
                     /locus_tag="VFB37_04550"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HZO21753.1"
                     /translation="MSGTGQRWSSELGFLLSAVGAAVGLGSIWRFPYLAAAGGGFAFI
                     AVFVLACLAFGAPVLVGEIITGRWSRHSPPQAAGIIAARSGYSTRWNVVGWSGSIAGY
                     LILSYYTMIAGWVLAYTWFFLSGNYARGGTAPAVAKFHAFVADSVSVSLWQLGFFALV
                     TFVSARQLNRGVEWANRWRAPALLAILLVLVVYSLATGDVARGLRFAFAPDVSRLTPA
                     VVLGAVGQALFALGVSAGIMIAYGAYMPAGESLRRSVLVVVGSIFIVSLLATVVIFPL
                     MFHYGLDPAEGPELVFQVLPVAFAQMPGGRLIGTLFFALLALAAFTPSVGLMEPWIAW
                     LSERAGLQRRTAAILCAGICWLIGQGSVLSFGRWSQWHPAASLPHLSGLNVFGLVDLL
                     SANVLMPISALLVSVFIGWRMGHRVPEEELSGLSASARRWLLFAMRYICPVGIAVVLL
                     VGFWH"
     gene            complement(10098..12170)
                     /locus_tag="VFB37_04555"
     CDS             complement(10098..12170)
                     /locus_tag="VFB37_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZO21754.1"
                     /translation="MSDAAQAGPGRLQQIWIGLDTGGTYTDAVALDSGMGIVASAKAP
                     TTHWDLSVGLGAAMRALLACLPVGWGREQVALVSVSTTLATNAVVESRFSPVCTFLIG
                     FDDRMVERSGLSRDAAGVVVRVQGGHDATGEELIALDEAAIDTAVREHASRVEAFCVA
                     AMFSVRNPAHELRARERIRAASGKPVTCSHELSSQLDAPKRALTAALNARLTPHIRHL
                     LEALERVLQEEAIAAPLMIVKGDGSLMRAEVALEYPVETVLSGPAASVVGAGVLTGRR
                     DFVVADMGGTTTDVAVVVAGHAVVREEGAIIGAWRTMVEAIDARTIGLGGDSEVGFDR
                     DGRLKVGPRRVMPLSRLAHEFPETLCVLQRLASAERVSPPATQLAFRNPGAAATGRLD
                     RLERRAWEALSPAPRPLAEIAHSNQGVQALHRLVDRGLVTLAGFTPSDAMHVLGHQHG
                     WNREAACLGSAILATEERSAGDRLERDTPQGISVRTSQYVIREAARLVLESTLAQDPG
                     IEARNGRWGPLGALLDQVVSGGTFSRLMEMRVRLATPLIAIGAPAKIYYPDIASRLGA
                     ELLVPEHAAVCNAVGAAAGVVSQTCEVLVNQPTLNVFRVHDPTGARDYTGPHEAIEDA
                     RRVSRELALAAARRAGASDPHVETSVTERRAHSQSGLDYLAEATVRSKATGRASTGRP
                     AGPERPAG"
     gene            complement(12187..13521)
                     /locus_tag="VFB37_04560"
     CDS             complement(12187..13521)
                     /locus_tag="VFB37_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO21755.1"
                     /translation="MPRNSIWLIRLIPFLFLIPMSKAATPAALPAPPSPVTVIRAGKL
                     IDVEAARVLSDQLIVIRDGKIQAVGANIQTPPDAKLIDLSSMTVLPGLVDCHTHLADL
                     ASAEPLTLLQMSAAETAYAAIPNARVTLLAGFTTVRDVGVYRAFNDVAMRDAIAKGII
                     VGPRMYVAGSYITISLGAGAMTGLAPDIQLPLDLRYGEANSPWEVRQKIRELAHRGAD
                     HIKILTTGAVLTHGSNPKSIEFTPEEIQAAVDEARNFGLRVAAHAHAPEGIKNAIRAG
                     VASVEHATLIDDEGIALAKKYGTYLDMDIYDEECIQSAPDTPADFLQHDRDLAEAQRR
                     NFTKAVKAGVKMTFGTDAGVCPHGINARQFAFMVKYGMTPMQAIQAATVNAADLIGHS
                     ELFGSIRAGKSADIIAVPGDPLSNIRLLENVSFVMKEGAVYKQDGHPTPAVL"
     gene            13679..14170
                     /locus_tag="VFB37_04565"
     CDS             13679..14170
                     /locus_tag="VFB37_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO21756.1"
                     /translation="MRARLLPALAAALISANVLGGMADQATDHSSGFVRLAPEQVRWQ
                     TVAEGFGAQIATIAGDPARAGLYVQRVRFPPHVMTRPHWHATDRYVTVLKGTWFAGEG
                     PTFDPRHAQPMKPGSYMFHPARALHWDGSNSDEEVIVQVFGMGPLESVRAEPGKPAWA
                     RVP"
     gene            complement(14204..14479)
                     /locus_tag="VFB37_04570"
     CDS             complement(14204..14479)
                     /locus_tag="VFB37_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO21757.1"
                     /translation="MKATSHLQVKTGRVVVTEWRFQPGAETGHHAHAYDYVVVPLTDG
                     VLRLEEPSGAREVRLQTGVSYARSAGVTHNVINANAHEFRFIEIELL"
     gene            complement(14476..14919)
                     /locus_tag="VFB37_04575"
     CDS             complement(14476..14919)
                     /locus_tag="VFB37_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZO21758.1"
                     /translation="MNDAQVLQGTAHPPVEIDWVSGIAFDAGRPGAHKVRIDADVRDA
                     PGPFDLLLGALAACAASDVVMILQKQRATLRSLHVNIEAKRVSGTPRHLASVVLHFIV
                     RAQGWTPEKAGRAIELAVTKYCSVGASLRADAPITWTVDLEEAGP"
     gene            complement(15237..16046)
                     /locus_tag="VFB37_04580"
     CDS             complement(15237..16046)
                     /locus_tag="VFB37_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HZO21759.1"
                     /translation="MRDSIRAVAWLSFLATAYLMCAAALAPWISLPQLGSVGFTLVFV
                     LFAVSHCLELEGVPRTVVFFTVSAVVSYLMEEIGVRTGVIYGAYHYSDQLGAKLGHVP
                     VIIPLAWFMMIYPSWRVAGALAGDCLARPAVALTVQAALAALVMTAWDMVMDPGMAAA
                     GNWTWEQGGAYFGVPAHNYVGWLATTFPVYWCTGWLWQNVRGRAGPTCAFGILPIILY
                     ALFAIRYIATNSIPALQLVALFAMGLPALIALTRVIPEMRRPGAVLDAAAK"
     gene            16308..>17241
                     /locus_tag="VFB37_04585"
     CDS             16308..>17241
                     /locus_tag="VFB37_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZO21760.1"
                     /translation="MNLKAWIHKARELGASDLHAEAGTALVARIRGELRPIGEAVPQS
                     ELELLGKELLGAEAWRQFSLRGSADVSTAIAGVRCRISFYRTIRGAAIAVRLLAPSVN
                     DLRSCNLHPDLHRLIDASTGLVVVSGPTGSGKSTTLAALIEEINTSRSRHIITLESPL
                     EYLFSNRRSYIRQREIPTHSPGFEQALTDALRENPDVLVIGEMRTPEVMRLTLNAAET
                     GHLVLATMHSATCAEALSRICMSFPAELQGNIRAQLADSLVGVLCQRLDFLEAHHLLV
                     PRCELLLASSAARGTIRTGQFSNIANVIQSGGEDG"
BASE COUNT         3081 a         5642 c         5414 g         3104 t
ORIGIN      
        1 agccgtttca gatcggagct cacatgccgg tcatcgctcg aactcagggg cagtcggctg
       61 gcacgcagca ggtctttggc gatccacgcc tgggtgcggg ccgagcgcag cgcccgaatg
      121 actttctgcg cctccgggtg actcatcagc aaagacaggt aggcgagcgc agccttgtag
      181 tcttccgctg cgggcttctc tctccagggc acttctttct tggacatagc gctgcatccg
      241 attccggcgt gcgctcattc tagcggtccc caagtgccgt gtatcgctag ccgcaggctg
      301 gcagcacccc ctccatggtg tagaggcgag cccggtgtct tccctgacga catggcagca
      361 gagttttggt atgggctcgc cagatccccc gcttggcgtg cgacacagat tagcggaagg
      421 gcagcgatcc gctatcggta taaacacgta cgcaaacggg aggcacagcg gccttgtggc
      481 cgcggcctca ggcattcgaa cgagcttcaa ctagctcatt gcagcggtag agcgtccaca
      541 tcaatgccgg tcgcctgccg gtagaccgcg agcagccttt ccactgatgg ctcgctgtcc
      601 tcatcggtga gcaccgtcca caactgcgcg accgcatcga ggtccggcac ataccggaat
      661 gtcacgcaca caaacattcc accgggctga tgcgtaaacc agtaagcgca ttgtcggcgc
      721 tcgtcgtact ccatcccgtt gcggccgggc agttccgcca gctcacgggc ttcgacggca
      781 atcgccgccg gcaaaggcat cagcaaagct tccatcgcgg cagagtcatc gatacacgcg
      841 cgtgccgccc atatagcctg ttcatctatt gttagttctg gggccatact tcgtgcctcc
      901 cgactcacac actacagcga atcgcgacac ccgacccctt cccgccgaca tattcccgga
      961 ccaaggggcg ctgagtgcct gcgggggggc cctccagctc gcagtcgatc acgttatgta
     1021 agggtagcgc cgggtactgg agcaattccg gtacccggcg gagcaacact tgctgccctg
     1081 cgggatgcgt cccatctgct gccaaggtcg ccgtcttgcc gcccgggcgc aaccgggagg
     1141 caagctacgt cccctcgcct tactgcccga tcaaatgcag tgcgatttcc ctggcatgcg
     1201 gccggtttcg gtgctcgaaa agatagattc cctgccacgt tccgagcgca agcttgccgt
     1261 cgatgacggg cacggaaagc tggacgccgg tcaaggcggt tcgaaggtga gccggcatat
     1321 cgtcgggacc ttcgtcttca tgccgataac gaccaggctg ctccggcgca agggtttcga
     1381 agaatgcctc caggtccgtg cgcacctcgc gcgcagcatt ttcctgtatc aacagagagg
     1441 ccgatgtatg acggcagaac agggtcaaca gcccggcact gatcttctga ctgcgcaccc
     1501 agccctgaac ttcgtccgtg atttctagca gcccacgccg atgcgggtgt attttcagga
     1561 tctgggtaat gttgtgcatt gcagcagagt tttgccccgc gatccctccg ccaccgccgc
     1621 aacgcactct ggctcgtcgc cgaggaacga gctgaccagc ttgcccaatg ccgtgacgcc
     1681 ccttgtgatc gtgccatgat cctgagtcgt ataactcatg cgcgcatggt tgatctcttc
     1741 ggtcaccgga aagaaggtcg cacccggcac gtacgcaact ttcaccttcg gcagcgcctc
     1801 gttggcgaga aactgcgtgg tatcgaaacc tgccgggaaa gtgagccacg tgaacatccc
     1861 accatgcggg ttggtacagg acaccgcacg cggaaaactc ccccggatgg cggccagcat
     1921 cgcctccttc ttgcgccggt aggtttcccg gatgatctcg atgtgggcct cgacgtcata
     1981 acgctcgaga aagagtgagg tggcggccat gctcagcgtg ctgcactggg tgtcggcagc
     2041 cagcttcaac agacccagct cgtgaatcag ctcgggctca gccacaaccc aaccgaggcg
     2101 caatccgggc gcgagaattt tcgaaaagct gccgaggtag atgacccgcc cttccgtgtc
     2161 gagactcttc agagttggaa tcggctcacc ctcgaagcgg atttcccgat acggcgtatc
     2221 ctcgagcacg atcagatcga agcggttggc gagctcgatc aaccggcgac ggcgcgccag
     2281 actgagcgat accccggtcg ggttctggaa atcgggcatg gtatagagca atttcacccg
     2341 ggggtgctct gtgagcgcct cctcgagatg ctcggtgtcc atgccctcac gatccatgcg
     2401 gatggccaga tagccgggct catacgggtt gaaggcaatc agcgcgccga ggaatgttgg
     2461 attttcggtg atgatcagat ctccccggtc caccagcatc ttcgccacca ggtcgagacc
     2521 ctgctgcgcg ccttggagga tcaatacgtt ttcagccgag cagcgcacgc cctggcgagc
     2581 catgcgttcg gcgatctgct cacgcagccg gggcatcccg gtggacaggg tgtactgcaa
     2641 tgcactgcgc ccctggccga tgatcgcttg ctcgaacacg gcggacagct tctccagggg
     2701 aaacagcgcg gggtcgggat agccaccgcc gaatgagatg atctgcgggt cggcgcccag
     2761 gcgcagcagc tcacggatgg ccgaaggctt cacacgcgcc atgcgcgtgg cgagggcagc
     2821 tgccagcccg cgtatcactc cttcttgctc cagctgacat acgcagccgc gagcgaggcg
     2881 ccggcaaggt tcaccccgct cagctccggc aatcgcatca aagcgtcgga gatcagccgc
     2941 tcgccatagc gcagatcggt tttccacagc tccgcggctc tcgccccatc acgaatcgcg
     3001 atcgcatctg cgatctgcag atgcgtcgtc tgaaaccact ccgagacatt cagactgcga
     3061 atgccgacat acaggatccg cagggtcagg tcctgcagat gggtgatcgt ggcggcaaga
     3121 cgcgcattgc cggaacccgc tgcgatcagc aggttgaact cgcggttggc cttcaggaac
     3181 tccaccagct cgccgtgatt ggtgaattcg taccggctct gtgcgagctc gcgcagccgc
     3241 tgcaggtcgg gggccgcgag gccgagctgg gcgccgagct ccgcagcccg gggctcgagc
     3301 aggatgcgca tcccgtagat gtccctgaca tccttgagcg tcaggggtgc cacgcgcggg
     3361 ctgcgctcgt cggtcttctc gatcaatccc tcctgtgcca ggcgcagcag cgccgatctc
     3421 acggcggcct gccgcaggcc gaagcggctg gtgagcgagg cttcggaaag gcggctgcca
     3481 gggggcagtt gataaaagag gatttccgag cggatccgct gataggcctg ctcgcccagg
     3541 ttgctgctgc gatcgggcac cgagcgacga tcttcgattg cagagaaggg catgcgcacg
     3601 ggcgtcgaat ccgttctggt cgggactata tgttactgca gggctcaacc gatgtcgtgg
     3661 gcagcgcttt cgcaggttgt gcggctattt acaacatttt gttgcggtgc tagcttcatc
     3721 tcgcgcggga acgattcagt cgccgcttcc cgggcaggcc ccgcggggct ccggtatatc
     3781 caccgtgtgc cgcaagtcgc gcacggcttt cggggtgcgg cacatcgggg gaggcggtat
     3841 gggtgccagg cgcgagccaa tcgaatatga ggcggcctca ccggaagtgc gggccgtgta
     3901 tgacgacatc atggcgaccc gcaagatcga ttgggtcaat aacttctgga aggtgctcgc
     3961 gcacgatccc gtggcgctgc ggcgcatctg gaccaacatc aagcaggtca tggggccggg
     4021 cgcgctcgac ccgctcacca aggagctgct gtacgtggcg gtgagcgcat ccaacggctg
     4081 ccgttactgc atcgcttcgc acggcgccgc ggcgcgtaag aagggcatga ccgaggcaca
     4141 gtattccgaa ctgctcgcga tcgtcggcct ggcgaacgaa accaacaccc tggtgaccgc
     4201 gctcgacgtg gcggttgacc cgcagtttcg ctgagccttc tcggggaccg cctgagctct
     4261 gcgtctttca acacgacagg ataccaatat gggcgccgtt gacttcagga gcgtgagcca
     4321 tcaccgctgg gaccgtattc cggaggaaac cctcaagggc acgatcacgc gacgcctgat
     4381 cacgggcgag cggatgatga tcgcgcatgt ctatctgaag aagggcgatg acgtgccacg
     4441 tcattcgcac gacaacgagc agctcaccta catcctgcag ggcgcgctgc acttctggct
     4501 cggtgcgaac gatgagcggg agctcaccgt gcgcgccggc gaggtgttgg tcatccccac
     4561 caacgtgccg caccgcgcgc tggcgatgga agatacgctg gatgtggacg tgttcagtcc
     4621 gccgcgccag gactggctct ccggcaccga cgcctatttg aggaagtagt gtgaatctcg
     4681 ggttgcaggg tcgccgcgcg ctcgtgatgg gcggctcgag cggcctggga agggccgtgg
     4741 ctgaggctct ggtcgcagaa ggcgcacgtg tggcgatttg cgcacgtaac gctgcgcgtg
     4801 tggccgacac tgcaaccgcg ctaggcgtcg aaggtctcgt cggtgatctg tcggtcggcg
     4861 gcgcggccac gaatctggtg cgcgaggccg agagccgcat cggcaatttg gacgtgctgg
     4921 tcgtcaacac cggagggcct cctccggctc cttttagcgc tctgacggat gccacctgga
     4981 gagaggcgtt cgaaggcctg tggatgagca gcgttggtgc gattcgtagc gcgcttgccg
     5041 ggatgcgcac ccgacgctgg ggccgcatca tcgtcatcac atcggtcgcc gctcgcgagc
     5101 ccgttccaaa cctgatgctg tccaactcgc tgcgcgccgg cttgcacggc ctcgtcaacg
     5161 cgctcagcag ggaggttgcg gcggatcagg taaccgtcaa cgccctcatg cctggataca
     5221 cactgaccga acgccttgca gacctcaagc tcgatatggc tgcgctgact gcgcagattc
     5281 ccgcccgcag gcttggaggt ccccgggagc tcgccgcgct ggcggccttc ctggcctccg
     5341 agcaggcggg ctacgtcacc ggccaggcga tcgcctgcga tggtggactg ctgcagtcga
     5401 tctgacccca cccttcgcaa tcaaccggca ggacaaccat gaagaagtac ggctgcccga
     5461 aatcacacct gaagggattc gtcctcgtgc tgctcgcctg gaacgccccg gcactctggc
     5521 acgaggctgt cgcccagcag gctaccgcca ccgaggagtc cgcgagcggc ctgcaggagg
     5581 tcatcgtcac ggcgcagaag cgagcgcaga actcgcagga catcggcata gcgctgtccg
     5641 cagtgagcgg cgatcagctc gaggcactgg gcgccgtgac cgcgagtgac atcacgaaaa
     5701 cgatgccggc cgtggtactc acccagccga acggcccctc ctccttcagt ctgtcgatcc
     5761 gcggcgtgac gcagaacgac ttcgccgacc accaggagag cccggccgca atctatgtcg
     5821 atgacgttta tgtgagccag atggcgggac ttgccttcac catgttcgac atggatcgcg
     5881 tcgaggtgct gcgcgggccg cagggcaccc tgtttggccg caacgccacg ggcggcctgg
     5941 ctaacttcgt cacccgccgt cctacggacg actacagcgg ctttgcagac gtgactttcg
     6001 gctcgcataa cctcacgcat atcgagggtg ccgtcaacgg tcagctctgg cacgacgtcg
     6061 acgcccggtt ctcgttccag agcaaccact atgacccgct gttccacaat atctccggcg
     6121 gagcccccga tgcggagaac ggcaacgact ggtcgctgcg cggccagctc cttttcaagg
     6181 atatcggtgg cggcagcgtg ctgttcatag cgcggggcag ccgtgaagac gtgcatgccg
     6241 gctcgtggga ggaatacgcg acgaacccag gccctaacgg atatgacacg ctgctggccc
     6301 cgaatcagaa cgcttacggc acctgcaacg gttgcaacgc cagcggactc accaacagcg
     6361 gtccgttcgt gacccgtgac aatcaggccg gctatgcccg cctgaagacc gaaggcctga
     6421 ccggcaagta cactgccgat ctgagcggtg cgacgctcac gctcatcgcc gattactcgg
     6481 gactgagaaa ggactatcag gaggactcgg acgcatcgcc tttcacggtc ttccagttct
     6541 tcaacggcag caatgtgaac caggaatcac tcgaagcccg actgaatggc ggggatcaga
     6601 agctcaactg gacggctggt ctgtacggat tgcgtatcga tggcaagtac tacgagggtt
     6661 gggaagggcc ggccttcttc ggtgcccagg agttcaacac tccatcgaac ccgaacgcgg
     6721 catatgcagc tttcgatggc gcagccgggc cgtggcccta tggagcgttt cccttctaca
     6781 ccggcgcgcc agcggctcca aatggggcac tgccagcaac ccgggcgccc tactccctgc
     6841 tcacgaagtc atacgctgca ttcgggcagg tcgagtaccg catgaccgag ctgctgggtt
     6901 tcacgctcgg cgcgcgcttc accaaggacc agaaggatta tgactatacc tggtacccgt
     6961 acgaatattt tccgcaaagc ctgaccggcg agacggtgca gctgacgcgt ccaagcagcg
     7021 cactgctgaa cgtgtatgac gccagccgca gcgacaatct ttggagcggc aaggcgcagg
     7081 tggatttcca cctgacacag gacttcctgg cctacgtcag ctacaaccgc ggtgtgaaag
     7141 gcggaggctt ttccgctccg ctattcccgg caacgatcaa cgatctggcg actctgacgt
     7201 tcaggcctga aaccctcatc tcctacgagg tgggattcaa atcggagtat ctgaatcaca
     7261 cactgcggat caatggcgcg gcgtattact acgattatca cgactatcag gctctcatct
     7321 acacgctggg cctcgagcag ctgatactca atgccgatgc cacacacaag ggcaccgagg
     7381 ccgagatcga gtgggctccg acggaagcct gggacctcgg cctcgggatc tcgtacgtgg
     7441 atgcgatggt gaacaacatc cccggccgct gctgcacggc ccagggcgcg ccggtgtacg
     7501 gcaatttcgt ccccggcaat gccccacgct ggtctggcaa cgccatgatc cgctacacgc
     7561 tgcctctcgg tccgggccat gtggccttgc agatggacgg caactacctg tcgcgattct
     7621 ggttcaacct gggtgatgcg cccgcagtgg agcagcctgc cttcggcatc gccaatgccc
     7681 gggtaaacta caccccgtcc ggtggcaaga tcgagatcgg agtcgccgtc gagaatctcg
     7741 ccaacaagca ctacggagtg atgggcttcg acaacacctc gatcaatgga ctggcgcagg
     7801 tctacccggg aatgccccgc tggtacaagg cgcatatcaa ttacaagttc tgatacggca
     7861 cgagcggaga atgacgtgag aacaatagca accgcagcca gtggcactgt gctggcagtc
     7921 ctctacagcg tgtgtgcgac ggccgcgccc gccgcgcctc ccagcctgga tgagatctat
     7981 gccacgctgg ccaccaagcg cttcgtcgat ctgacgcacg ctttcggccc cagcacgcca
     8041 cactggaagg gctttggcga tgagacggtg aagacgctct acaccatcaa gaaggacggc
     8101 ttcaaggtgc agcagttcac tcatgtcggg cagtggggca cgcacatcga cccgcccgcg
     8161 cactttcacg agggactgcg cacggtcgat cagattccgc cgcaggagat gattctgccg
     8221 ctggtcgtgc tcgacgtgca cgagaaggcc gcgaagaatc cggactacac gatcaaggtc
     8281 gcggatgtga gcgcatggga ggcgaaacac ggccgcattc ccgagcacgc tttcgtagcg
     8341 atgcgcaccg attggtcaaa gcgctggccc gacgatgcgg ccatgcagaa tcgggactcg
     8401 gcaggagtcg cccactatcc gggctggagc aaggaagtgc ttcagctgct gtacgaagat
     8461 cgcaagatca ccgcctcggg tcatgagacg accgacaccg acccgggact cgcgaccacg
     8521 cacgatgact actcgctcga gcgttacttg ctgggcacga atcactacca gatcgagctg
     8581 ctcgccaacc ttgaccaggt tcccgaggcc ggtgctctgg tggtggtgac ctggcccaag
     8641 ccggaagggg gcaccggttt ccccgcgcgg gtattcgcca tcctgccgtg agcgccatcc
     8701 tgccgtgagc cggccacccg caagatgagt ggcacgggcc agcgctggtc atcggaactc
     8761 ggatttctgc tgagcgcggt gggcgctgca gtggggctcg gcagcatctg gcgctttccc
     8821 tatctggcag cggcgggcgg cggtttcgct ttcattgccg tattcgtgct ggcgtgcctg
     8881 gcgtttgggg cgccggtgct cgtcggcgag atcatcacgg ggaggtggtc gagacattcg
     8941 ccgccgcagg cggcaggtat catcgcggcg cgctcaggct actcaacacg ctggaacgtc
     9001 gtcggctgga gcggctccat tgccggctat ctgattctct cgtactacac gatgatcgcg
     9061 ggctgggtgc tcgcgtatac ctggttcttc ctttccggca actacgcccg cggcggaacc
     9121 gcgcctgccg tggcgaagtt ccacgccttc gtcgccgact cggtcagcgt ctccctgtgg
     9181 cagctcggtt tttttgcgct ggtgaccttc gtctcggcgc gacagctcaa tcggggcgtc
     9241 gaatgggcca accgctggcg cgcaccagcg ctgctggcga tcctgctggt gctggtcgtt
     9301 tactcccttg caaccggcga cgtcgcgcgt gggctgcgct tcgccttcgc gcccgatgtc
     9361 agcaggctta cgccggctgt cgtgctgggc gccgtgggtc aggcgctgtt tgctctcggg
     9421 gtgagtgctg ggatcatgat cgcttatgga gcgtacatgc ccgccggcga atcgctgcgg
     9481 cgcagtgtgc tcgtggtcgt gggctcgatc ttcatcgtct cgctgctcgc caccgtggtg
     9541 atctttccgc tcatgttcca ctatggactg gatccggccg agggcccgga gctggtcttt
     9601 caggtgctgc cggtcgcgtt tgcgcagatg ccgggcggtc gtctgatcgg caccctcttc
     9661 ttcgctctgc tggccctcgc ggccttcacc ccctccgtgg gtctgatgga accgtggatc
     9721 gcctggctct ccgagcgcgc cggactacaa cgcagaaccg ccgcgatcct ctgcgcgggc
     9781 atctgctggc tcatcggaca gggatcggtc ctgtcgttcg ggcgctggtc ccagtggcat
     9841 ccagccgctt cgctcccgca cctgtcaggc ttgaatgtct tcggtctcgt ggatctgttg
     9901 agcgctaacg tcctgatgcc gatctcggcg ctgctcgtga gcgtgttcat cggttggcgc
     9961 atgggccatc gcgtgccaga ggaggagctg agcggcctct cggccagcgc ccgtcgctgg
    10021 ctgcttttcg caatgcgcta catctgcccg gtgggcattg cagtggtgct gctcgtcggg
    10081 ttctggcact gaaagcatca ccccgccggg cgctctggcc ccgcggggcg gcccgtcgaa
    10141 gcgcgccccg tcgctttgga gcgcacggtc gcctccgcca gatagtcgag cccggactgc
    10201 gagtgcgctc ggcgctcagt gactgaggtt tcgacgtgcg gatcgctggc gcccgcgcgc
    10261 cgggccgccg caagcgcgag ctcgcgcgac acgcgccgtg catcctcgat cgcctcgtga
    10321 ggccccgtat agtcgcgcgc accggttgga tcgtgcactc ggaagacatt gagagtgggc
    10381 tgattgacga ggacttcaca cgtttgcgag acgacgccgg cggcggcacc gaccgcattg
    10441 cagaccgctg cgtgctccgg tacgagtaac tcggcaccca ggcggctcgc aatatccggg
    10501 taatagatct ttgccggggc tccaatggcg atgaggggcg tcgcaagccg gacacgcatc
    10561 tccatgagcc gcgagaaggt gcctccggag acgacctgat caagcagcgc ccccagcggt
    10621 ccccagcgtc cattcctcgc ctcgatgccg gggtcctggg cgagagtgct ctcgagcacg
    10681 agccgcgcag cctccctgat cacatactgc gaggtgcgga cagaaatgcc ttgcggagta
    10741 tcgcgctcga ggcgatcccc tgcgctgcgc tcctcggtcg cgagaatggc cgatcccagg
    10801 caggcggcct cgcgattcca tccgtgttga tgcccgagca catgcatcgc atcactcggt
    10861 gtgaatccgg ccaaggtgac aaggccgcgg tccaccagcc ggtgcagcgc ctgcacgccc
    10921 tgattgctgt gcgcaatctc ggccagcggc cgtggagcgg ggctgagcgc ctcccacgcg
    10981 cggcgctcga gccgatccag ccggcccgtg gcggcagcgc cgggattgcg aaaggcaagc
    11041 tgcgtggcgg gtggcgaaac tcgctcggcg gaggccagcc gttgcaacac gcagagcgtt
    11101 tccgggaact catgcgccag tctgctgagc ggcatcaccc ggcgcggtcc caccttgagc
    11161 cggccatccc gatcgaaacc gacctccgag tctcctccca gtccaatcgt gcgcgcatcg
    11221 atggcctcga ccatggtgcg ccaggcaccg atgatcgcgc cttcctcccg aaccaccgcg
    11281 tgtcctgcga cgaccaccgc cacatcggtc gtagtgccgc ccatgtcggc gaccacgaag
    11341 tcccgccgac cggtcaacac ccccgcgccg accacgctgg cagcgggtcc cgacaggacc
    11401 gtttccaccg ggtactcgag tgcaacctct gctcgcatca ggctgccatc gcccttcacg
    11461 atcatcaagg gcgcggcgat ggcctcctcc tgcagcaccc gctcgagagc ctcgagcaga
    11521 tgtcggatgt gcggagtcag gcgcgcattc agcgcagccg tcagcgcccg cttgggcgca
    11581 tcgagctgcg agctcaactc atggctgcag gtcaccggct ttccagatgc cgcacggatc
    11641 cgctcccgag cacgtaactc gtgcgccggg ttgcgcaccg agaacatcgc ggccacgcag
    11701 aaggcttcga cccgagaggc atgttcgcgg accgccgtat cgatggccgc ctcgtccagg
    11761 gcaatcaact cctcgcccgt cgcatcgtgt ccaccctgca cgcgcactac aacgccggct
    11821 gcatcacggc tcagcccgga gcgctcgacc atccgatcgt cgaacccgat gaggaaggtg
    11881 cacaccgggc tgaagcggct ttcgaccacc gcgttggttg caagggtggt tgacacggaa
    11941 accagcgcca cctgctcgcg accccaaccc acgggcaagc aagccaacag ggctcgcatc
    12001 gccgcaccca ggccaaccga gaggtcccaa tgtgtggtgg gtgccttggc actggctact
    12061 atgcccatgc cactgtcgag tgcgacggca tcggtgtaag tgccgccggt atccaggccg
    12121 atccagatct gttgcaggcg tccgggccca gcctgcgcgg catcgctcat cggcatccga
    12181 tctctgctaa agcaccgctg gcgtcggatg accgtcctgc ttgtaaacgg ccccctcctt
    12241 catcacgaag gacacgttct cgagcaggcg gatgttgctc agtggatctc ccggcacggc
    12301 gatgatgtcc gcggacttgc ctgcccggat ggagccgaac agctcggagt ggccgatcag
    12361 gtcggccgca tttaccgtgg ccgcctggat cgcctgcatc ggggtcatgc cgtacttcac
    12421 catgaaggcg aactgcctcg cgttgatgcc gtgcggacac acgcccgcat cggtgccgaa
    12481 tgtcatcttg accccggcct tcacggcctt ggtgaagttg cgccgctgcg cctcggcaag
    12541 atcccggtcg tgctgcagga agtccgccgg agtgtccggc gcggactgaa tgcattcctc
    12601 atcgtagatg tccatgtcca ggtaggtgcc atacttcttt gccagagcaa tcccctcgtc
    12661 atcgatgagc gtggcgtgct cgaccgaggc gaccccggcg cggatggcat tcttgattcc
    12721 ctcaggcgcg tgcgcgtgag cggcgacccg gaggccgaag ttgcgtgcct catcgactgc
    12781 ggcctggatc tcctcgggtg tgaactcgat cgacttcgga ttgctgccgt gggtcaggac
    12841 ggcgcccgtg gtcaggatct tgatgtgatc ggctccgcgg tgcgcgagct cgcggatctt
    12901 ctgccgcacc tcccacgggc tgttggcctc accgtagcgc aggtccaagg gcaactgaat
    12961 gtcgggggcc aggccggtca tcgctccggc gcccaggctg atcgtgatgt acgaccccgc
    13021 cacgtacatg cgcggaccga cgatgatgcc tttggcgatg gcatctcgca tcgcaacgtc
    13081 gttgaatgcc cggtacacac ccacatctct cacggtcgtg aagcctgcaa gcaatgtcac
    13141 gcgcgcgttg ggtatggcgg cgtaagcagt ctcggctgcc gacatctgca gcagtgtgag
    13201 gggctccgca ctcgcgagat ccgccaggtg ggtgtggcag tcgacgagcc ctggcagcac
    13261 ggtcatcgat gagagatcga tgagctttgc atcgggcggc gtttgaatgt tggcacccac
    13321 cgcctggatc tttccatcac ggatcacgat gagctgatcg gacaggacgc gcgccgcttc
    13381 gacgtcgatc agctttccgg cccggatcac cgtcaccgga gacggcggag ccggtagagc
    13441 cgctggtgtg gcggccttgc tcatcggtat cagaaagaga aagggaatca gacgaatgag
    13501 ccagatactg tttctgggca cgacaaatcc tctcagcaca gcccccgcgc gctgcgcttg
    13561 ccaccttagc acagccggac gaatcagcgc tgaccaccgc ctccgcactc atcgcaggct
    13621 cgccttgcgc agggtcccct gttaaatttt gccgtcttgc ttcccggagg cgtccaacat
    13681 gagagcgagg cttctgccgg ctttggctgc ggcgctgatc agcgcaaatg tgctgggcgg
    13741 aatggccgat caggcgaccg atcattcctc aggattcgtg cgcctcgcgc ccgagcaggt
    13801 tcgatggcag acggtcgcgg agggctttgg cgcgcagatc gccacgatcg ccggtgaccc
    13861 ggcccgagcc ggactctacg ttcagcgggt caggtttccc ccgcacgtca tgacgcggcc
    13921 ccactggcat gcgacggacc ggtacgtcac ggtcctcaag ggcacgtggt tcgcgggcga
    13981 aggtccgaca ttcgatccgc ggcacgcgca gccgatgaag ccgggaagct acatgtttca
    14041 tccagcacgc gcgctgcact gggatgggtc caacagcgac gaggaggtca tcgtccaggt
    14101 gttcggcatg ggccctctgg agagcgtgcg tgccgagccc ggcaaaccgg cctgggcgcg
    14161 tgtcccttag ccgcctgccg atcgctcctg cgccgggata ggctcagagc agctcgatct
    14221 cgatgaaccg gaactcgtgg gcattcgcgt tgatcacgtt gtgggtcaca ccggcagagc
    14281 gagcgtagct gacgcccgtt tgcagtcgca cttcccgcgc ccctgagggc tcctcgagcc
    14341 gcagcacgcc atcggtcagc ggcacgacca catagtcgta cgcatgcgcg tgatgtccgg
    14401 tctccgctcc gggctggaag cgccactccg tcacgacgac acgcccggtc ttcacttgca
    14461 ggtgggatgt cgctttcacg ggcctgcttc ctcgagatcg accgtccagg tgatcggcgc
    14521 atccgctcgc agcgatgcgc caacgctgca gtacttcgtt accgcgagct cgattgcgcg
    14581 gccggccttc tccggcgtcc aaccctgggc ccgcacgata aaatgcagga ccacggaggc
    14641 caggtgtcgg ggtgtaccgc tgacccgctt tgcttcgatg ttgacatgca gtgagcgcag
    14701 ggttgcccgc tgcttctgca gtatcatcac gacgtccgac gcggcgcatg cagccagggc
    14761 gccgagcaga agatcgaagg gcccgggcgc atcgcgcacg tccgcatcga tgcggacctt
    14821 gtgcgccccg ggccgacccg catcgaacgc gatgcccgat acccaatcga tttccacagg
    14881 cgggtgcgcg gtgccttgca ggacttgtgc atcgttcatg tgtgactgaa tcggccagcc
    14941 gaggggccgt tagcctcgcg catgaccgcc agcggcgcaa gcgcaggcgc accacgcctg
    15001 atccaaggaa agcgatccac agagacacga tggcgtactc tttgccgtcg ctgacgttat
    15061 ggccgactat cggtgcggct acgcttcccg atttttccag ccagtaggcg tacagaacgc
    15121 gcagcaggaa tgcgtaagtc tgctctccga gggcgatcgg ccagttgcgt acacgaccac
    15181 cagaagcgga aggaccttga agcgcggccg cgctcccgag agctccaggc tcatccctac
    15241 ttcgctgccg catccagcac cgccccgggg cggcgcattt ccgggatcac gcgagtcaac
    15301 gctatgagcg ccggcagtcc catggcgaac agcgcgacga gctgcaatgc agggattgag
    15361 ttcgtagcga tgtaccgtat cgcgaagagt gcgtacagga tgatcggcag aatcccgaac
    15421 gcgcaggtcg ggccggctct gccgcgaacg ttttgccaaa gccagccggt acaccagtag
    15481 accggaaagg tcgtcgccag ccagccgaca tagttgtgcg caggcactcc gaagtatgcc
    15541 ccgccctgct cccaggtcca gttgcccgcc gcagccatcc cgggatccat caccatatcc
    15601 cacgccgtca tcacgagcgc ggcgagcgcc gcctgcaccg tcagggccac cgcaggccgc
    15661 gcaagacagt ccccggccag cgctccggca actcgccagg acggatagat catcatgaac
    15721 caggcgagcg ggatgatgac cgggacatgc ccgagcttgg caccgagctg atcactgtag
    15781 tggtaggcgc cgtagatcac tccagtgcgc acaccaatct cctccatgag gtaggacacg
    15841 acggccgaaa ctgtgaagaa gaccactgtg cgcggcacgc cctcgagctc gaggcagtgc
    15901 gatacggcga acaggacgaa caccagcgtg aatcccaccg agccgagttg cggcaggctg
    15961 atccagggag cgagcgccgc ggcgcacatc aggtacgccg tcgccagaaa gctgagccag
    16021 gccacggccc ggatcgagtc gcgcatacgc tctccgcccg agcctttaaa ccaggcgcag
    16081 cccgctttgc ctgatcatgc aatccccctt gttgcatcac ctcgagagat cgaccgatcg
    16141 ctgcggtggt gcccgattag cttaccgccc tcgaaccgcg ccaccaaccc tgcctggcca
    16201 gcgcagtgtc ctgtgcccga atcaggcgcc ctgtacaatc tccccatcac catggctgcg
    16261 gccggaaacg gaccagcgca tcgcgccgca gccggcggga tctgcatatg aatctgaagg
    16321 cctggattca caaagcccgg gagctggggg ccagcgacct gcacgccgag gcaggcaccg
    16381 cgctggtggc gcgcatccgc ggtgagctgc gtcccattgg cgaagccgtt ccgcagagcg
    16441 agctcgagct gctcggcaag gagctgctcg gcgccgaggc ctggaggcaa ttcagcttac
    16501 gcggctcggc ggatgtttcc acggcgattg cgggcgtgcg ctgccggatc agcttttatc
    16561 gcacgattcg gggcgccgcg atcgcggtcc ggctgctggc gccctcggtc aatgacctgc
    16621 gctcgtgcaa tctgcacccg gatctgcatc gtctcatcga tgcgagcacg gggctcgtgg
    16681 tggtatcggg tccgaccggc agtggcaaat ccacgacgct ggcggctttg atcgaggaga
    16741 tcaacacctc gcgttcccgc cacatcatca ccctcgaaag cccgctcgag tatctcttct
    16801 cgaaccggcg ctcctacatc cgccagcgcg agatccccac gcactcgccg ggcttcgagc
    16861 aggcgctcac cgacgccctg cgcgagaacc cggacgtact ggtcatcggc gagatgcgca
    16921 cgccggaggt catgcgcctc acgctgaatg cggcggagac cggtcatctg gtgctcgcca
    16981 caatgcactc cgcgacctgc gcggaagccc tgagccgcat ctgcatgtcc ttcccggcgg
    17041 agctgcaggg caacatccgc gcgcagctgg cggacagcct cgtcggtgtg ctctgccaac
    17101 gcctggactt tctcgaagcg caccatctgc tcgtgccgcg ttgcgagttg ctgctggcaa
    17161 gctccgccgc gcgcggcacg atccgcacgg gccagttcag caacattgcc aacgtaatcc
    17221 agtccggggg cgaggacgga a
//
LOCUS       DASRHN010000120        15949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_368866, whole genome shotgun sequence.
ACCESSION   DASRHN010000120 DASRHN010000000
VERSION     DASRHN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368866"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFB37_04590"
     CDS             <1..201
                     /locus_tag="VFB37_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21761.1"
                     /translation="RVAAVRVLVVTGRRDGTSAHGKMQENPMFSSTCVANPQRQLRYA
                     IPAAFLHGAMRHSNADLRQSGR"
     gene            complement(242..1276)
                     /locus_tag="VFB37_04595"
     CDS             complement(242..1276)
                     /locus_tag="VFB37_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21762.1"
                     /translation="MATQPITATCLVLLAACAAPALAQQAQTPPPPAPAPSSSGATYD
                     AAEHLSILPKNGQTTDQIWADRYTCHNWARTQSGFDPTRPPQSASSGDLAAQHDQYRR
                     ALSACLESHGYNVSTEAQAAGTNIPPPPPAGSQHLERMHHRLYPMTGFNYHPLTLGID
                     AGYTITAGNAGRSLDDGYNTGLSINWFPSSTVPLGLRINGSYMRFNENLNSVFAVERQ
                     TGLGDLFGHQDLYGGDVDLQLNLRMGPSVREYFFGGVGWYRQQTRFEQLSFDRGFVCF
                     FNCFRGVFPVGNTVQENTTGWTHSWNAGMGFEFALTDPTSFFIEARYMRLGDMSSKQE
                     FVPITIGLRF"
     gene            complement(1330..2301)
                     /locus_tag="VFB37_04600"
     CDS             complement(1330..2301)
                     /locus_tag="VFB37_04600"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HZO21763.1"
                     /translation="MHLPESLLAIACGIFLPLTCVWAEAASDRGTPEQSQSRGPQEQS
                     EAYHKHHDRRHGHNHVYPDRGSILREVPSGAALVNYAGVSYRFYDGIWFEPRGPAFMV
                     VLPPIGLVVPSVPSFATVVDAGGEPYLYADDVYYRARPDLGGYEVVNDPADMATGAAA
                     ANGPSAAPPATAPPAAATAQPPAGAPLPAATTPPTSATAPPAVATSAAPPAVAPGSAG
                     AAPAPTVLSQPAAAAANAPAAPPQPSTALPVLTVGSAGGRVLAVPRSAKSQDEQERDH
                     YDCYRFAAAQSGFDPMRTNPPFAQATGQANYERAQAACFEARGYTVR"
     gene            complement(2327..2497)
                     /locus_tag="VFB37_04605"
     CDS             complement(2327..2497)
                     /locus_tag="VFB37_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21764.1"
                     /translation="MAVRQGRWIVVPLHERTWRFFTGTLIFQIEVNDGQERCVAPALF
                     IQLHSGGDQAEP"
     gene            complement(2747..4450)
                     /locus_tag="VFB37_04610"
     CDS             complement(2747..4450)
                     /locus_tag="VFB37_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO21765.1"
                     /translation="MRSDKGASAFVAARDLLLRLRDDPDRAAAEFRWPPLHEFNWATD
                     YFDPLAASIERDAVRVVGDDGEQQVLTYRGLSRRSSQLANYLRACGIRAGDRVLLMLG
                     NDIALWECILALMKLRAVMIPATTLMQREDLRDRLERGSVSAIIAESDCASRFEDLPG
                     AAIRIAVGPAASGWLDFAQSRNASMEWVRLPCRADDLLLLYFTSGTTARPKLVAHTHS
                     SYPVGHLSTLYWIGLRPGDVHLNLSSPGWAKHAWSCVFAPFNAEATVLAYRYERFDAR
                     RLLEVLTAQRVTTFCAPPTVWRMLIQHDMSGHRGVLRELVSAGEPLNPQVIDHVRRIW
                     KLTIRDGYGQTETTAMAGNSPGSRIKDGSMGRALPGYRIELLDAEGNPAQEGEICIPL
                     AGRATGLMQGYLDSGETPARPTDAEYYRTGDVAVRDEEGYLTYVGRMDDVFKSSDYRI
                     SPFELESVLIEHEAVAEAAVVPSPDPMRLAIPKAYIVLTAGHAQNAETALSIFRHVHA
                     RVAPYKRIRRIEFAPLPKTISGKIRRVDLRAAEHPRSGQEHHPGEFWEEDFPALRSPQ
                     P"
     gene            complement(4733..6391)
                     /locus_tag="VFB37_04615"
     CDS             complement(4733..6391)
                     /locus_tag="VFB37_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21766.1"
                     /translation="MSSPLKGATWTLLLLFAVLRGAPAVEPTGRGEAHPDPAASRVPE
                     SEPDSVTEGSVTVDGHAIRYRAVSGTLTVGSTNELDALLGTDGHWLPNSGFKVAAEDP
                     QNAPATARIFYVAYFRTGAPAAGRPVIFLYNGGPSVATVWLHMGAFGPRRVSIPDTQH
                     QSAAPYAIIENKYSLLDVADLVFIDAPGTGFSRVMGRNAERAFWGVDEDAHAFERFIH
                     RFLTKYDLWNNPKYLFGESYGTPRTAVLAAALRQVDLNGIILLSTILSMDDSIDAVKG
                     NPGVDQGYALVLPSYAATAYFHHKLSPQPAALLPFLQEVEDFALGEYMAALLQGSQLS
                     AARKQAVAEKLHQYTGLPADLFVRYDLRINVGIFCKRLLQEQGLEIGRLDTRYTGLNL
                     NPGSEEADATLDPMSLATGPAYYAAVNKYLKEDLKFGANDSYKFSLYDVPGFRWDWHH
                     QGPGSPAPLFAYTGTSMSLDLAYAMKENPKMKILVAGGYFDLGTPFFGGLYEMHHLPM
                     PPQLQANISYRYYETGHMLYLNEGALRQFHEDVAAFVHATEIGQ"
     gene            6499..7308
                     /locus_tag="VFB37_04620"
     CDS             6499..7308
                     /locus_tag="VFB37_04620"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZO21767.1"
                     /translation="MKVSTFLQVTAGTLLLSVSVSALADNAVTSRPANVLAGPDDSYP
                     VVAQLDADTPVQVMGCLNDWSYCDVAFQGARGWLYAPDLTYAYQGGYVPLYSYGPALG
                     ITVESFSVDNYWGRYYHDRPWYSRREEFDRRGPPHHERPPGPAPSHSPPPREVVQHRQ
                     DNHSGGVRLSSAGSDSRHQENERRAGSAENRAPAPRPEERAAPRPEERTAPRPEERAP
                     AARPETRAKPAEHPTPQREERRTASPNDSKHEEQPPKEERKDRPDHPDRPQ"
     gene            7827..11072
                     /locus_tag="VFB37_04625"
     CDS             7827..11072
                     /locus_tag="VFB37_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21768.1"
                     /translation="MRRSRSGYWLMVVAALGSMAATVVSAADSDKESDFKFRFVGPKV
                     GNRIAAAVGIPGDPSTYYAGAASGGVWKSTDGGNRWAPVFDKQSAAAIGALAVAPSEP
                     STVWAGTGEAWAIRDSDVMGNGIYKSTDAGHTWINMGLPQSGRIGRIIIHPSNPNIVF
                     ACVLGRTTGPQQERGVYRTTDGGQHWERVLFGGENVGCSGLSMDPHNPYALVAGMWEV
                     QMHTWGEFSGGPGSGVYISHDGGSKWTRIEEHGLPAPPVGKIDVAIAPTNSKRIYALI
                     QTKDQGSVWRSDDRGENWKRVNSQRALIGRAGYYIRIAVSPASDNEVLVANSSFHQSL
                     DGGENFHEVRWGGDTHDIWFDPTDADRFVITDDAGMLITTVHGRGFHRVHLPIGQMYH
                     VAVDDQIPYYFYSNMQDDGNMRGPVVPLDSDETGWDRHMGGCESGFTVPDVADPNIVW
                     ATCYGDTVTRWDAHYKEAHSVSPWKHTLDSPPNDVKYRCHWTPPLAVDPFDPNTVYYG
                     CQVIFRTTNAGQSWSVISPDLSTHNPAHIVPSGGLVGDNLGQFYGDVVFAIAPSSIKK
                     GLIWAGTNDGQIWNTQDAGANWTNVSGHLPGLPAGGTVTRIAPSTFDAATAYVSVDLH
                     LENNRDPFIYKTTDSGKSWKRITANLPKDELSYVRTVTEDPNCQGLLFAGTGNSLYYS
                     LDDGGHWTALKTGLPPAPVTWAVVQKRFHDLVVSTYGRGLYILDDISPLEQMAKHHSE
                     GPVALFEPRQTYRFVRGGEALLNFSLPAEHKDAIHLEILDAQGNEIRKLEAKELLAGL
                     NRVKWDLHYDSPRVIALRTVAPDNPRIWDEPRFRDEDSRPITHWGSKPAEVGPIVAPG
                     TYSVRLTVDGKSYTQPLTILRDPRAPGSDADIQASVKTLLKIRDDISQASDTVNQVEW
                     LRKQLEVIEAMLRPAQESDKHEAPITEEEDEYDSQPPPAPPRELSDVEKQQREQLLQA
                     TQDLDKKLESVESRLVSHALRNSDDKYFVEAYGVYMDLIWLNAEVGSGGGDVAGGADF
                     APTDAQLETLKTLETQMSSADSDLKQVLDHDLPALDQGLEHANIAPVAHSP"
     gene            11200..11694
                     /locus_tag="VFB37_04630"
     CDS             11200..11694
                     /locus_tag="VFB37_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZO21769.1"
                     /translation="MKPRIEFRNANPKIMPLLLGIESYLQGSSLGSRLLHLVKTRASQ
                     INGCAYCLDMHTKDARAEGDSEQRLYALDAWRETPFFDERERAALEWTEAVTRVADTH
                     VPDDVYERVSEQFSEEELIDLTLAVTNINAWNRLNVAFRTVPGNYRAGMYKSTMSAAE
                     RPRA"
     gene            11707..12540
                     /locus_tag="VFB37_04635"
     CDS             11707..12540
                     /locus_tag="VFB37_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZO21770.1"
                     /translation="MRTQRELYERFRALHEQKGAFVMPNPWDAVSALLLKEAGFQALG
                     TSSAALASALGRLDGRHAVSRDEHLANARLIGELTDLPVNGDFEDGYGRSASDVAATV
                     QAAIAGGLAGIGIEDTSADPARPIRDFDEAVERVRQAAKAANGRILLTGRTDNFLQGR
                     PDLEDTIKRLVAFAEVGADVLYAPYPPDMEAVRSIVRAVSPKPVNIVVGTMSGAVPVK
                     ELEAAGVKRISMGVALYTRVMADLRKAAQQLAEGHSDLASAGLPFGEIKKMIAKATAA
                     K"
     gene            12577..15027
                     /locus_tag="VFB37_04640"
     CDS             12577..15027
                     /locus_tag="VFB37_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO21771.1"
                     /translation="MADAVPEWADVVIVGGGIVGCSVAYHLAEQGCTNVVLLERRELT
                     CGTTWHAAGLVGQLRATLNLTRLAQYTASLYARLEAETGHATGFRQTGSIAIAGSVAR
                     FEELKRGASMGRSFGLEVAVLSPSDVKALWPLVAIHDLAGAVYLPKDGQTNPVDTTQA
                     LARGARKRGVQVIEHCRVTHILRADDRVIGVCTERGDIRSEIVVNCGGMWARDIGGWA
                     DASVPLHAAEHFYIVTQPVPGLTPGLPVLRDPDSCAYFKEDAGKILAGWFEPVAKPWG
                     HDGIPESFSFDHLPDDLAHMEPLFEAAVRRVPQLESAGVQLFFNGPESFTPDDRYLLG
                     EMPEVRGLFVAAGFNSIGVQSAGGAGKVLADWIVKGHAPMDLWDVDIRRCVPFQRNKR
                     YLRDRTVEALGLLYAMHWPFRQPETARGVRKSALHDRLQAAGACFGEVAGFERPNWYA
                     PAGVPAKYEYSYGRQNWFEYSATEHRAVRERVGLFDQSSFAKFLVEGRDAEPVLNGLC
                     TANVDVPVGRIVYTQWLNERGGIEADVTITREAPHRFLVITSCATQTRDLAWLRRHIP
                     ETARAVSVDVSAAYAVLGIMGPQSRALLSSLTDSDLSNDAFPFGSSQVIDLGYARVRA
                     TRITYVGELGWELYIPTDCAQGVYDEILAAGQQFGLVHAGYHAMNSLRMEKGYRHWGH
                     DIGAEDTPLEAGLSFTVHWSKPGGFLGLAALLRQQDEGVRRRLVHFMLDQADPLLYHD
                     EPIWRDGTLVGRLTSGMFGHTLGRPLGMGYVADCGRVVTPDWVDAGRYEIEVGLQRIG
                     ARASFQPFYDPGSQRVRS"
     gene            15141..>15949
                     /locus_tag="VFB37_04645"
     CDS             15141..>15949
                     /locus_tag="VFB37_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZO21772.1"
                     /translation="MTTRRGALGLIGLALPAWAFAARGQSEKEAPAVAVGDPSIKVIE
                     NVWIPMRDGSRLAARLFLPQTAGKTPTGAVLEYLPYRKRDAYRYRDDVAGPSLARSGI
                     ALLRVDIRGTGDSDGAMVDEYMPIEQADALAVIEWMAKQPWCNGNVGMRGISYGSFVA
                     LQAAAKAPPALKAIVSTCGTEQRYLDDIHYRGGCLINDQYVWAMEWQVIMRAPPDPII
                     VGEDRWRPMWQQRLEAASAMTILWNEHQTLDGKWKDGSIQDYGRIRCAIYNV"
BASE COUNT         2973 a         5020 c         5135 g         2821 t
ORIGIN      
        1 cgcgtcgcag cggttcgagt tcttgtcgtg acgggcaggc gggacggtac atcggcacac
       61 ggcaaaatgc aagaaaaccc tatgttttcc agcacttgtg tggccaatcc gcagcggcag
      121 ctgcgctacg cgatccccgc agcgttcctg cacggcgcga tgcgccactc gaacgccgat
      181 cttcggcagt ctgggcgcta gagtgagcgg tgccctcttg cctcgagagc gcctcggctc
      241 ttcagaagcg cagcccgatc gtgatcggta cgaactcctg cttgctgctc atatcgccca
      301 ggcgcatgta gcgtgcttcg atgaagaagc tggtcgggtc cgtcagcgcg aactcgaacc
      361 ccatgcccgc attccacgag tgtgtccagc cggtcgtatt ctcctgaacg gtgttcccca
      421 ccggaaagac gccgcggaag cagttgaaga aacagacgaa gccgcggtcg aaggaaagtt
      481 gctcgaagcg ggtctgctgc cgataccagc cgaccccgcc gaagaaatat tcccgcacgc
      541 tcggtcccat gcgcaggttg agttgcagat cgacatcccc gccgtaaagg tcctggtggc
      601 cgaacagatc gcccaggccg gtctggcgct caacggcgaa cactgaattg aggttctcat
      661 tgaatcgcat gtagctgccg ttgatccgca ggcccagtgg gaccgtggag gaaggaaacc
      721 agttgatgga cagtcccgtg ttgtagccat catcgagcga tcgccccgca ttgcccgcgg
      781 tgatggtgta gcccgcgtca attcccaggg tcaacgggtg atagttgaat cccgtcatcg
      841 ggtacaaacg atggtgcatg cgctcgagat gctgcgaacc ggctggaggg ggcggtggga
      901 tgttggtgcc cgccgcctgc gcctcggtac tgacattgta tccatgcgac tcgagacacg
      961 cgctcagtgc gcgccggtac tgatcatgtt gtgcggccag atcgcccgag gacgcgctct
     1021 gcggaggcct cgtcgggtcg aagccgctct gggtccgcgc ccagttgtga caggtatagc
     1081 ggtccgccca gatctgatcg gtcgtctggc cattcttcgg caagatggag aggtgctcag
     1141 ccgcatcgta ggtcgccccc gaggaggacg gcgcaggcgc aggtggcggc ggggtttgcg
     1201 cctgctgcgc cagtgcgggt gcagcgcagg cggccagcag aactaggcag gtcgctgtga
     1261 tgggttgggt ggccatggcg ttactcctgt acataagccc gtcgcatcaa catttccgtc
     1321 cggactggat caccggaccg tatatccgcg cgcctcgaag caggccgcct gtgcacgctc
     1381 gtaattggcc tgcccggtcg cctgtgcgaa aggaggattg gtgcgcatag gatcgaaacc
     1441 gctttgcgcg gcggcgaagc ggtaacagtc gtagtgatcg cgctcctgct cgtcctgaga
     1501 cttggcactt cgcggcaccg caagcaccct gccgccagcc gatccaaccg tgagaaccgg
     1561 caaagccgta gaaggctgag gcggtgctgc aggtgcattc gcggctgcgg cggccggttg
     1621 ggagagaact gtcggtgcgg gcgcagcacc cgccgagccc ggggccacag cgggtggcgc
     1681 cgctgaggta gccacagcgg gtggcgcagt cgcagaagta ggtggtgtcg tcgcggcagg
     1741 gagtggcgca cccgcaggag gctgcgcagt cgcggcagca ggcggcgcag tcgctggcgg
     1801 cgcggccgac ggcccgttgg ccgcggcggc cccggtcgcc atgtctgccg gatcgttcac
     1861 gacttcgtag ccgcccagat ccggccgggc acgatagtag acgtcgtctg cgtatagata
     1921 cggctcgccg cccgcatcaa ctactgtggc aaaagatgga acgctgggaa cgacgaggcc
     1981 gatcggcggc aggaccacca tgaatgccgg cccgcgaggc tcgaaccaga tcccgtcata
     2041 gaatcgatac gacactcctg cgtagttgac cagcgcggcc ccgctcggca cctcgcgcag
     2101 gatcgagccg cgatccggat aaacatgatt gtggccgtgc cggcgatcgt gatgcttgtg
     2161 gtaggcctcg gactgttcct ggggcccgcg actttgactc tgttccggcg tgccgcgatc
     2221 ggatgcggcc tcggcccaga cacaggtgag tggaagaaag atgccgcagg cgatagccaa
     2281 cagtgactca ggcaggtgca tcgtcgccct cccttccgga cgaagctcac ggctccgcct
     2341 gatccccgcc cgaatgcaat tggatgaaca gcgcaggagc aacgcatctc tcctgcccat
     2401 cgttgacctc gatctggaaa atcagagttc ccgtgaaaaa tctccacgtc cgttcgtgca
     2461 gaggcacgac gatccagcgt ccctgcctca cagccatcga ctcgctgtac cttgaaccaa
     2521 cacgaggcgc gcgggcgccg cgtgctcgcg atctgagcgc aaatccttcg cgcccagtgg
     2581 cagggacatg ccatctgcat gcattgttcc tgcaatgagc ttccggattg ttccccgcac
     2641 gcagggccag acggaacgca gggggtaaga tcacgcagcg attttggatc ggctgaaggt
     2701 cgagctgcgg ctcgctcgat gcggccgcgc atccgccggc agcgccttac ggctggggcg
     2761 agcgcagcgc gggaaaatcc tcttcccaga attcgcccgg atggtgttcc tgcccactgc
     2821 gcggatgttc cgcggcgcgc agatccaccc ggcggatctt tccggagatc gtcttgggaa
     2881 gcggcgcgaa ctcgatgcgg cggattcttt tgtacggcgc gacacgggca tgcacgtgcc
     2941 ggaagatcga cagcgccgtt tccgcatttt gcgcgtgtcc ggccgtgagg acgatgtagg
     3001 ccttcgggat cgcgagccgc atcggatccg gactgggcac caccgccgcc tcagctaccg
     3061 cttcgtgctc gatgagcacg ctctccagct cgaacggact gatgcgatag tccgaggact
     3121 tgaagacgtc atccatgcgg cctacgtagg tcagatagcc ttcctcgtca cgaaccgcaa
     3181 catcaccggt acggtaatac tctgcgtccg tcggccgcgc gggggtctcc ccactgtcga
     3241 gatagccctg catcaagccc gtggcgcgcc ctgcgagcgg aatgcagatc tcaccctcct
     3301 gcgcggggtt accctcggca tcgagcagct cgattcgata tcccggcagc gcacgaccca
     3361 tggatccatc cttgatcctg ctcccgggcg aattgcccgc catggcagtg gtctcggtct
     3421 gaccgtaccc atcgcggatg gtcagcttcc atatcctgcg cacgtgatcg atgacctgcg
     3481 gattcaaggg ctcaccggca ctgaccagct ctctgaggac accgcggtgg cctgacatat
     3541 cgtgttgtat cagcatccgc cacacggtgg gtggtgcgca gaatgtcgtc acgcgctggg
     3601 cagtcaggac ctcgaggagc cgccgggcat cgaagcgctc atatcgataa gcgagcaccg
     3661 tcgcttcagc attgaatggc gcaaacacac acgaccaggc atgtttcgcc caccctggcg
     3721 agctcagatt caaatgcaca tcgcccgggc ggagtccgat ccagtacagg gtcgagaggt
     3781 gccccaccgg gtagctgctg tgggtatgcg caacgagctt gggtcgagcc gtggtgcccg
     3841 aagtgaagta tagaagcagc aggtcatctg cgcggcacgg cagccggacc cactccattg
     3901 aagcgttgcg cgactgcgcg aagtccagcc agcccgaggc cgccggcccc accgcgatcc
     3961 ggatcgcggc gccgggcaga tcctcgaacc gggaggcgca gtcgctctcg gcgatgattg
     4021 cgctcacgct gccgcgctcc aggcggtcgc gcagatcctc gcgctgcatg agagtggtcg
     4081 ccggaatcat caccgcgcgc agcttcatca gcgcgagaat gcattcccag agagcgatgt
     4141 cgttaccgag catgagcagt acacgatcac cggcacgaat gccgcaggca cgcaggtagt
     4201 tggccagctg cgacgaacgg cgcgaaagcc cgcgataggt gagcacttgc tgctcgccgt
     4261 catcgccgac gacgcgcacg gcatcgcgct cgatcgaggc cgccagtgga tcgaagtagt
     4321 ccgttgccca gttgaattcg tgcagcggtg gccagcggaa ctcggcggcg gcacgatccg
     4381 gatcgtcccg cagccgaagc agcaggtcgc gcgccgcaac gaaagcggac gctcccttgt
     4441 cggacctcat gcgcatcagc tcacctgaga caccggcgga ttcgaaccgc ggatcacctc
     4501 gaggcgaggc tacgaaacgt gccgctttct atcaagcgcg agcggctcga acgtcttttt
     4561 cggccgggcc cgtttcgcaa cgcgtgagag ccgcactccc cgggttcgca gagcgcccga
     4621 attgagggcg gccgcggtga ccctgacggc aggatccgct gtgggcttgc cttcttacgc
     4681 gcgagctgca tttgggcggc gagggcgccg cagtgcggga gatggagggc cgctactgac
     4741 ctatctcggt agcatgcacg aaagccgcta cgtcctcgtg aaactgacgc agggcaccct
     4801 cgttcagata cagcatgtgc ccggtctcgt agtagcgata gctgatgttg gcctgcagct
     4861 gtgggggcat cggcaggtga tgcatctcgt agagaccgcc gaagaacgga gttccgagat
     4921 cgaagtagcc gcctgccacg aggatcttca tcttcgggtt ctctttcatc gcataggcga
     4981 gatcgaggct catgctggtg cccgtgtagg cgaagagcgg cgccggactt cccggtccct
     5041 ggtggtgcca gtcccagcgg aagcccggga cgtcatagag ggagaacttg tagctgtcat
     5101 tggcgccgaa cttcagatcc tccttcaggt acttgttgac tgcggcgtag taggcgggcc
     5161 cggtcgccag gctcatcgga tcgagcgtgg cgtcggcttc ctcactcccc ggattgagat
     5221 tgagtcccgt gtaacgggta tcgagcctgc cgatctccag accttgctcc tgcagcaggc
     5281 gcttgcagaa gatcccgaca ttgatccgca ggtcgtaacg gacgaacaga tcagccggca
     5341 agcccgtgta ctgatggagt ttctccgcca cggcctgctt tctggcggca ctgagctggc
     5401 tgccctgcag gagagcggcc atgtactccc cgagcgcgaa gtcctcgacc tcctgcagga
     5461 aaggcagcaa cgccgccggt tgcggcgaga gcttgtgatg gaaatacgcc gtcgcagcgt
     5521 acgaaggcag tacgagcgca taaccctgat cgacaccggg gttacccttg accgcatcga
     5581 tggagtcgtc catggagagg atggtggaga gcaggatgat tccgttcagg tccacctggc
     5641 gcagcgccgc cgccagcacc gcggtgcggg gcgtgccata actctcccca aacaggtact
     5701 tggggttgtt ccacagatcg tacttggtca ggaagcggtg gatgaatcgc tcgaacgcat
     5761 gagcgtcctc gtcgacaccc cagaaggcac gctcggcatt gcgacccatc acccgactga
     5821 acccggtacc gggggcatcg atgaagacca gatcggcgac atcgagcagg ctgtacttgt
     5881 tctcgatgat tgcgtaagga gccgccgact gatgctgggt atccggaatt gaaacccgcc
     5941 gcggaccgaa cgctcccatg tgcagccata cggtcgccac actcggaccg ccgttgtaga
     6001 gaaagatgac cggccggccc gccgccggcg ccccggttcg aaaataggcg acgtagaaaa
     6061 tccgcgcggt ggccggcgcg ttctgcggat cctccgccgc aaccttgaat ccggagttcg
     6121 gcagccagtg accatccgtg cccagcagcg catccagctc attggtcgag ccgacggtca
     6181 gagtgccgga gaccgcccga tagcggatcg cgtgtccgtc cacggtgacg gagccctcgg
     6241 ttaccgagtc cggctcgctc tccggcaccc ggctggcagc cgggtcggga tgagcctctc
     6301 ccctgcccgt gggctcaacg gctggcgccc cccgcaggac ggcgaacagc agcaggaggg
     6361 tccaggtcgc gcctttcagg ggggagctca tggagtgctc actttcactg tttacaactg
     6421 caccgacaag ctctgtcagc gcggaacccc agggggctgt aaggcattgt agccatagct
     6481 atggctgcgg aggtcttcat gaaagtctca acttttctgc aggtgacagc gggcacgctg
     6541 ctgctctccg tttctgtgtc cgcacttgcg gacaatgcgg taacttctcg tcctgccaac
     6601 gttcttgccg gtccggacga ctcctatccg gtggtcgcgc aactcgatgc cgatacgcct
     6661 gtgcaggtca tgggctgtct gaatgactgg agctattgcg atgtcgcctt ccagggcgcc
     6721 cgtggatggc tttatgcacc ggatctgacg tacgcatacc agggcgggta cgtgccgctc
     6781 tattcctacg gcccggcact tggcatcaca gtcgagtcct tctcggtcga taactactgg
     6841 ggccgttact accacgatcg tccctggtat tcccggcgtg aggagttcga tcgccgcggt
     6901 ccgccgcacc acgagcgacc gcccggcccg gcgcccagcc attcacctcc gccacgtgaa
     6961 gtggtgcagc accggcagga caatcattcc ggcggcgttc gcctgagcag cgccggatcg
     7021 gactcccggc atcaggagaa cgagcgccgt gccggcagtg ccgagaacag ggcccctgcg
     7081 ccgcggccgg aagagcgtgc agcgccgcgg ccggaagagc gtacagcgcc gcgacccgag
     7141 gagcgcgcgc ccgcggcacg ccccgagact cgcgcgaaac ccgccgagca tccgacgcct
     7201 cagcgggagg aaaggcgcac tgcatctccg aatgactcca agcatgagga gcagcctccg
     7261 aaggaggagc gcaaggatcg tccggaccat ccggatcgtc cgcagtaatc gcgaagtcag
     7321 tgcaagcaga acatccggtg ggatgatccc cgccggatgt tctgcgtcct gggagccact
     7381 caccgaggcg cttgcccttc agcgcagggg cacctgcagt tcggtccggt actcagttcg
     7441 gagctgcagg ggcccgtgtc gtgggcaccg gccagcgcga tggcgatcgc ctggtcgtgt
     7501 tatttccata cgcgagggcg ccttccgcga tacgttcacc cgcgattcag cggacgattc
     7561 gtggcagctg ctgctggaat cgcaggaaaa gagcggctcg tggtcaacgt tcgcatcgta
     7621 cgtgctcacg cgtccgcaca agcactgatc cgagcggcac ttcgtgccca ccgcgaccga
     7681 ccttcacagg cggtttccaa agaattccaa gcaaggaata tctctgcagt caggcacggc
     7741 cgtcttctgc ccggcagtcc tgctccaggg cgtgcgaact gatgtaaggt gcgccctttc
     7801 cctgccgcgg tcggaaggag tagcccatgc gcagaagcag atccgggtac tggctcatgg
     7861 tggtcgccgc tctcggcagc atggcggcaa ccgtcgtatc ggcagccgat tccgacaagg
     7921 aaagcgactt caaattccgc ttcgtggggc ccaaggtcgg caaccggatt gccgccgcgg
     7981 tgggaattcc gggcgacccg agcacctact acgcgggtgc ggcctccggc ggagtatgga
     8041 aatccacgga tggcggcaac cgttgggcgc ccgtattcga caagcaatct gccgccgcaa
     8101 tcggtgccct cgccgtcgcg ccctccgaac caagcaccgt ctgggcgggc accggggaag
     8161 cctgggccat ccgcgacagc gacgtgatgg gcaacggcat ctacaaatcg accgatgccg
     8221 gacatacctg gatcaacatg ggacttccgc agtcgggtcg catcgggcga atcatcatcc
     8281 atccgtccaa tcccaacatc gtgttcgctt gcgtgctggg ccgcacgacc ggccctcagc
     8341 aggagcgtgg cgtctatcgc acgactgacg gcggccagca ttgggagcgc gtgctgtttg
     8401 gtggagagaa cgtcggttgc tcgggacttt ccatggaccc ccacaatccc tacgccctcg
     8461 tggccggcat gtgggaggtg cagatgcaca cctggggtga gttcagcggc ggtcccggca
     8521 gcggcgttta catttcgcac gacggtggat cgaagtggac ccgcatcgaa gagcatggtc
     8581 tgcctgctcc gccggtgggc aagatcgatg tggcgatagc cccgaccaac tcgaagcgca
     8641 tttacgcgct catccagacc aaggatcagg gctcggtgtg gcgttcggac gaccggggag
     8701 agaactggaa gcgtgtcaac tcgcagcggg ccctgatcgg ccgtgcaggc tactacatcc
     8761 gcatcgccgt atcgcccgca agcgacaacg aggtgctggt ggccaacagc tcattccacc
     8821 agtccctcga tgggggcgag aacttccacg aagtccgctg gggcggagac acgcacgaca
     8881 tctggtttga cccaaccgac gccgaccgtt tcgtcatcac ggatgatgcc gggatgctca
     8941 tcacgacggt ccacgggcgc ggcttccacc gtgttcatct gccgatcggg cagatgtacc
     9001 acgtggccgt ggatgaccag atcccgtact acttctatag caacatgcag gacgacggaa
     9061 acatgcgtgg ccccgtcgtg ccgctcgact cggatgagac gggatgggat cgccacatgg
     9121 gcgggtgcga atcggggttc acggtccccg acgtcgcgga tccgaacatc gtgtgggcga
     9181 cctgctacgg tgacacggtc acccgatggg atgcgcacta caaggaggcg cattcggtca
     9241 gtccgtggaa gcacacgctc gactcacctc ccaacgatgt caaataccgc tgtcactgga
     9301 caccgccgct ggccgtcgat cccttcgatc ccaacaccgt ctactacggt tgccaggtga
     9361 tcttccggac cacgaatgcg ggtcagagct ggtcggtgat cagccccgat ctgtccaccc
     9421 acaatccggc gcacatcgtt ccctccgggg gactcgtggg agacaacctc ggtcagttct
     9481 acggcgacgt cgtcttcgcc atcgcgcctt cctcgatcaa gaaaggtctg atctgggccg
     9541 gtacgaacga cggccagatc tggaataccc aggacgcagg agcgaactgg accaacgtct
     9601 cggggcatct ccccggattg cccgccggcg gcacggtgac ccgcatcgct ccttcgacgt
     9661 tcgatgcggc tacggcatac gtgagcgtcg atctgcacct ggaaaacaat cgcgacccct
     9721 tcatctacaa gaccaccgac tccggcaaat cctggaaacg aatcaccgcc aatctaccga
     9781 aggacgaact gtcctacgtt cgaaccgtga cagaagaccc aaactgtcag ggtctgttgt
     9841 ttgccgggac tggcaacagt ctgtactact cgctcgatga tggcgggcac tggacagccc
     9901 tcaagacggg gttgcccccc gctcctgtga cgtgggccgt cgtgcagaag cgctttcacg
     9961 atctcgtggt ctccacctac ggccgcggcc tgtacatcct ggatgacatc tcgcctctgg
    10021 agcagatggc aaagcatcat tccgaaggtc cggtggcgct gttcgagccg cgtcagacct
    10081 atcggttcgt gcgcggcggc gaggcactgc tcaacttctc gctgcctgcc gagcacaagg
    10141 acgccattca tctggaaatc ctcgatgctc aggggaacga gatccgcaag ctcgaggcaa
    10201 aggagctgct tgcaggactg aaccgggtga aatgggacct gcactacgac tcgccccgag
    10261 tgatcgcctt gcgcacggtc gcgccggaca atccgcgcat ctgggacgag ccgcgctttc
    10321 gcgacgagga ttcccggccg atcacgcact ggggatccaa acctgccgag gtgggtccga
    10381 tcgtcgcgcc gggcacctat tcggtccgtc tgaccgtcga cggcaagtcc tacactcagc
    10441 cgctgacgat cctgcgcgat ccccgcgcgc caggctcaga tgcggacatt caggcctcag
    10501 tcaagacact gctcaaaatc cgcgacgaca tcagtcaggc ttccgatacc gtcaaccagg
    10561 tcgaatggct gcgcaagcag ctggaagtga tcgaggcgat gcttcggccg gcccaggagt
    10621 cggacaagca cgaggcgccc atcaccgagg aggaagacga atacgactcg cagccgccgc
    10681 cggctccgcc gcgcgagctg agcgatgtgg aaaaacagca gcgcgagcag ctcctccagg
    10741 caacgcagga tctcgacaag aagctggaaa gtgtcgagtc gcggctcgtc tcgcatgcgt
    10801 tgcgcaacag cgatgacaag tacttcgtcg aggcctatgg cgtttatatg gacctgatct
    10861 ggctgaatgc ggaggtcgga tccggtggcg gcgacgtggc gggcggcgcc gatttcgcgc
    10921 ccacggatgc tcagctcgag actctgaaga cgctcgagac ccagatgtcg agtgccgatt
    10981 ccgacctcaa gcaggtgctg gatcacgatc tgccggcgct cgatcagggg ctggagcacg
    11041 cgaacatcgc acccgtggcg cactctccgt gaggggcggt cggcacggtt tggaatccgg
    11101 acaagcttgc ggggccgcct gcgacggcgc acggttaatg accctgtcac acgcaggacg
    11161 caccgttcgt ctatggagca gcaccccaag gagattctta tgaagcctcg catcgaattc
    11221 cgcaacgcca atcctaagat catgccgctg ttgctcggca tcgaaagcta tctgcagggg
    11281 tccagtctgg gctcgcgcct gctgcacctc gtcaagacgc gcgcctcgca gatcaatggc
    11341 tgcgcctact gcctcgacat gcatacgaag gatgcccgcg ccgaaggtga ctccgagcag
    11401 cggctttacg ccctcgatgc ctggcgcgag acgccgttct tcgatgagcg cgagcgcgca
    11461 gcactcgaat ggacggaggc ggtgacgcga gtggccgata ctcacgttcc ggacgatgtg
    11521 tacgagcggg taagcgagca attcagtgaa gaagagttga tcgatctgac gcttgccgtc
    11581 accaacatca acgcctggaa ccgtctgaac gtcgcctttc gtaccgtgcc gggaaactat
    11641 cgagcgggga tgtataaatc cacgatgtcc gcagccgaac ggccgagagc ttgaaggagg
    11701 gctgccatga gaacccagcg tgaactgtac gaacgattcc gcgcactgca cgagcagaag
    11761 ggcgccttcg tcatgcccaa cccttgggac gcagtctcgg cactgctgct caaggaggcc
    11821 ggatttcagg cgctgggaac ctcctcagcg gcactggcct cggcgctcgg aaggctggat
    11881 ggccgccatg cggtgagccg cgatgagcac ctggcgaacg caagactcat cggcgagctc
    11941 acggatctgc cggtgaacgg agacttcgag gacggctacg gacgaagtgc cagtgacgtc
    12001 gccgcaacgg tgcaggcggc cattgcgggt ggactcgctg ggatcggcat cgaggacacg
    12061 agcgccgatc ccgccaggcc gatccgggac ttcgatgagg ccgtcgagcg cgtgcgccag
    12121 gcggccaagg cggccaacgg ccgcattctc ctgaccgggc gcacagacaa tttcctgcag
    12181 ggcaggccag acctcgagga caccatcaag cggctcgtcg cttttgcaga ggtcggagcc
    12241 gacgtcctct acgcgcccta ccctcctgac atggaggccg tccgctcgat cgtgcgggcc
    12301 gtatcaccca agccggtcaa catcgtcgtg ggcaccatga gtggcgcagt gcccgtcaag
    12361 gagctcgagg ctgcgggcgt caaacgcatc agcatgggag tggcgcttta cacccgcgtg
    12421 atggccgatc tgcgcaaagc cgcacagcag ctcgccgagg gtcactctga tttggcttcc
    12481 gccggcctgc ccttcggcga gatcaagaag atgatcgcga aagcgaccgc cgcgaagtag
    12541 cacaaggcgc ggcgcgaagg ggaggcaagg caaagcatgg cggatgcagt gccggagtgg
    12601 gcggacgtcg tcatcgttgg cggcggcatc gtcggctgca gcgtcgccta ccacctggct
    12661 gagcagggct gcacgaatgt cgtgctgctc gagcgtcgcg agctgacctg cgggacaacc
    12721 tggcatgccg ccggactcgt tgggcagctg cgtgccacac tgaacctcac ccgactggcg
    12781 caatacaccg cctcgctcta cgcgcggctg gaagcggaga ccggtcacgc gacaggattc
    12841 cggcaaaccg gttccattgc gatcgccggg agcgtggcgc gcttcgaaga gctcaagcgc
    12901 ggtgcatcga tgggccgcag tttcggactg gaagttgcgg ttctctcgcc gtcggacgtc
    12961 aaggctctct ggccactcgt agcgatccac gacctggcag gggcggtcta tctgccgaag
    13021 gacgggcaga ccaatccggt cgatacgacg caggccctgg cccgcggagc ccgaaaacgg
    13081 ggcgtgcagg tcatcgaaca ctgtcgcgtt acccacatcc tccgggcaga tgaccgggtg
    13141 atcggcgtct gcaccgagcg cggcgacatt cgtagcgaga tagtggtgaa ctgcggcggg
    13201 atgtgggcac gtgatatcgg cggttgggcg gatgctagcg ttccgctcca cgccgccgaa
    13261 cacttctaca tcgtgacgca gccagtccct gggcttacgc ccgggctgcc cgtgttacgc
    13321 gatccagata gctgcgccta cttcaaggaa gacgccggta aaatcctcgc aggatggttc
    13381 gagccggtcg ccaagccctg gggccacgac gggatccccg agagcttctc cttcgatcat
    13441 ctgccggacg atctcgccca catggagccg ttgttcgagg ccgccgtccg gcgcgtccca
    13501 cagctcgagt cagcgggtgt gcagctcttc ttcaatggtc cggaaagctt cacgcccgat
    13561 gaccgctacc tgctggggga gatgcccgag gtgcggggac tcttcgtggc cgccggattc
    13621 aactccatcg gtgttcagtc tgccggcggt gccggcaaag tcctggcgga ttggatcgtc
    13681 aagggtcatg caccgatgga cctgtgggat gtggacatcc gccgctgcgt ccctttccag
    13741 cggaacaagc gctatctgcg cgaccgaaca gtcgaagcgc tgggattgct gtacgcgatg
    13801 cactggccct tccgccagcc ggagaccgcg cgcggcgtgc gcaagtccgc gctccacgac
    13861 cggctgcagg cggcgggagc ctgcttcgga gaagttgccg gcttcgagcg tcccaactgg
    13921 tacgcacctg ccggagtgcc agcgaagtac gaatacagtt acggtcgcca gaactggttc
    13981 gagtactcgg ccacggagca tcgcgcggta cgtgagcgag tcgggctgtt cgaccagtcc
    14041 tcattcgcga agttcctggt cgagggtcgc gatgccgagc ccgtcctcaa tggactgtgt
    14101 acggccaacg tggatgtgcc ggtcggcagg atcgtctata cgcagtggct caacgagcgc
    14161 ggcggcatcg aggcggacgt gacgatcacc cgcgaggcgc cgcatcggtt cctcgtgatc
    14221 acgagctgcg ccacgcagac ccgcgacctc gcctggctgc ggcgccatat tcctgagacc
    14281 gcgcgcgcag tatccgtgga tgtctccgcc gcttacgccg tcctgggaat catgggccct
    14341 caaagccggg ctttgttgag ctcgctcacc gactccgatc tgtcaaacga tgcttttccg
    14401 tttggcagct cgcaggtgat cgatctgggc tatgcgaggg ttcgtgcgac tcgcatcact
    14461 tatgtgggtg agctcggctg ggaactctat attcccacgg actgtgcaca gggtgtctac
    14521 gatgaaatcc tggcggctgg gcagcagttc gggctggtgc acgcgggata tcacgcgatg
    14581 aactcgctgc gcatggagaa aggctatcga cactggggcc acgacatcgg cgccgaagac
    14641 acgccgctcg aggcgggcct ctctttcacc gtgcactggt ccaagcccgg cgggttcctt
    14701 ggactggcgg cgctgcttcg tcagcaggac gagggtgtgc gacggcggct ggtgcatttc
    14761 atgctcgacc aggccgatcc gcttctctat cacgacgagc cgatctggcg cgacggcacg
    14821 ctggtgggac gtctcacctc gggaatgttt ggccacacgc tcgggcgccc tcttgggatg
    14881 ggctacgtcg ccgactgcgg gcgggtcgtg acacctgatt gggtggatgc cggtcgctac
    14941 gagatcgaag tcggcctgca gcgcatcggt gcgcgggcct cattccagcc tttttacgac
    15001 ccaggcagtc aacgcgtaag aagctgacat ggacactcac ccgtgcccgg ccttaacggt
    15061 gagtgggctc aggggtcaca cgatgaagct cgctagggta gagtacgccg ttcaataagc
    15121 ggcgagtgag gcttccgaga atgaccacga gacgcggagc tctgggactg attggattgg
    15181 cacttcccgc ctgggcgttt gccgcgcggg ggcagtcgga gaaggaagcg ccggcggttg
    15241 cggtcgggga tccttcgatc aaggtgatcg agaacgtgtg gatcccgatg cgggatggct
    15301 cgcgcctggc ggcgcgactc tttttgcccc agacggccgg caagacaccc acgggcgcgg
    15361 tcctggagta cctcccctat cgcaagcgcg atgcctatcg ttaccgcgat gacgtcgccg
    15421 gaccctctct tgccaggtcc ggcattgcgc ttctgcgtgt cgatattcgt ggcacgggcg
    15481 attccgatgg cgcgatggtc gatgagtaca tgcccatcga gcaggccgat gcgctggcgg
    15541 tgatcgaatg gatggcaaag cagccctggt gcaacggtaa tgtggggatg cgcggaatct
    15601 cctacggctc attcgtcgct ctgcaggcgg ccgccaaggc ccctcccgca ttgaaggcga
    15661 tcgtctcgac gtgtggaacc gagcagcgct atctggacga cattcactat cggggtggct
    15721 gcctcatcaa tgatcagtat gtgtgggcta tggagtggca ggtcatcatg cgcgcgccgc
    15781 ccgacccgat catcgtgggg gaagatcgct ggcgtcccat gtggcagcag cggctcgagg
    15841 ctgccagcgc catgaccatc ctctggaatg agcatcagac actcgatggc aaatggaagg
    15901 acggatcgat ccaggactac ggccggattc gctgcgccat ctacaacgt
//
LOCUS       DASRHN010000121         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2018122, whole genome shotgun sequence.
ACCESSION   DASRHN010000121 DASRHN010000000
VERSION     DASRHN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018122"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1377
                     /locus_tag="VFB37_04650"
     CDS             310..1377
                     /locus_tag="VFB37_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZO21773.1"
                     /translation="MILFGRLGDMVMVSSVLHPLHLRFQQPCLILGAGEWNAPLFSAQ
                     PDVAEVWSLPRHVPFFLSPVFWRALWSLRRCHPGPIYVCERQPRQLARVRRLLRLSGI
                     DPARCLFITDLAASHEEHWVDRFVRFSAGTPAALRASDYPLPRLQDVPAPVLCVTDSE
                     RTERDAWIRSQGWDGRPLILVQPGNRRSMSKRRERRQLNADDKLWPTERWASLLERIQ
                     GRMPEALILLCGTAREEALLGEIRAAARRAGVVEAKLPLRPLLALCEVAHSMISVDTG
                     PAHAAAALGLPLVVLFGAESPRRWLPRSALGSAVMGVGGPPERVRADQISVEDVFSIW
                     CELLAKKAAPYRHPARTYEGA"
     gene            1433..2563
                     /locus_tag="VFB37_04655"
     CDS             1433..2563
                     /locus_tag="VFB37_04655"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO21774.1"
                     /translation="MPSTALKNGCPLVCLWFAAAAAWLPAPCTLAAEPEPVKHEFIIK
                     NFKTESGAVLPEARIVYGTYGKLNAAGDNAMLLPSHYMAEMHGYAWLIGPGKALDPNR
                     LFLITSELFGNGRSSSPSNTAEPMHGPRFPVMTIRDNVHAVHELLGSLHVHHLRAVVG
                     FSMGAQQAFQWAVSYPDYMDRIVATSGTAKTYGHGIVRLEGQIAALTADAAFQGGNYS
                     TPPEKGLQAFAVVWTGWLFSQEWWRRELWRTDASLGSTFPQVLEHFRTKFIPGADAND
                     LILQMRTWESNDVGNTPPFAGNVETALRSIKAQVLYMPSETDLYFPLSDARYEAAFIP
                     HCSLVPIPSLWGHPAGAGASREDAKFLNEHIGAFLEGESATN"
     gene            2607..4775
                     /locus_tag="VFB37_04660"
     CDS             2607..4775
                     /locus_tag="VFB37_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO21775.1"
                     /translation="MQTLHRLHTGTAMLALAALLALPHPAAARTESAFTMADVLDYPY
                     TSELVAAEHADVIAWVRNLQGVRNVWVAKGPAYTPIQATHYTQDDGQELTQLTFTPDG
                     DHIVYVRGGDHDANWPAEGNLAPDPDSSTEQPETTIWSVGTDASAPVKIAEGDTPAIS
                     SRGQLAYTKDDHVWTVSLDGQGKAERLFFDRGKDSDLRWSDDGSRLAFVSDRGDHAFI
                     GIFSSKDRPISYLAPSTGNDSSPRWAPDGSRIAFVRQPGTGGAPEPLLKEVPHPWSIW
                     VANIADGAGHLVWQSPDTLAGSFPEVADGANLYWAAGDRLVFLAYLDDWPHLYSTPVA
                     GGAPVLLTPGSYMVEHVAQSRDRRFMVFDANTGKTQDDDDRRHLMRVAVDHPGPAELT
                     SGDTLEWAPVTTVSDHIAYVSAGFQQPPAPAVIELNGRHAPQRVGKDLPTDFPLKAFM
                     TPRSVSFKAADGLLVHGQLFDAGAGAGAGARGGGGKPGVIFVHGGPPRQMLLGWHYMD
                     YYSNAYAVNQYLAAHGFVVLSINYRLGIGYGRAYHFPEHAGPRGAAEYQDVVAGARYL
                     QSRPGVDPARIGIWGGSYGGYLTALALARDPDIFKAGVDLHGVHDWSALPDMPFSKAP
                     TRYEKGDREEAIHMAWESSPDASIDAWKGRVLLIQGDDDRNVPFQQTVDLARRLQAHK
                     VPYEELVIPNEIHGFLRHATWLHVDEATAEFLARTLGARH"
     gene            4850..>5097
                     /locus_tag="VFB37_04665"
     CDS             4850..>5097
                     /locus_tag="VFB37_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21776.1"
                     /translation="MSLLVRINVALAATFIIAAAALGYVCSTMLQANAKQNVLEQAGL
                     MIDSALATRDYTADEILPLLNAQMRTEFLPQSVPFYAAT"
BASE COUNT          880 a         1654 c         1639 g          924 t
ORIGIN      
        1 tcatcggtct tcattcggcc ttttctgctg caatctctcg ggcaattgtg tccgtggggc
       61 ccccgattcg ctagacttcc cgcaaatcat gagcttctcc acgggcgcaa ggggtgatcg
      121 aaccctcgcc acccttcgga agagaccttg cgcctgaaaa gagtgccacg cggctcaacc
      181 gggcagcaga tcgctgcgtt tgagacgact cctgccgaga cttgcccgta ccagaacgag
      241 gttggcgggc aaacgcccgt atcccccccg caggcgcctc gggcccgggc tccctcgttg
      301 cagccgaccg tgatcctctt cggccggctc ggggacatgg taatggtcag ctcggtgctg
      361 catcccctgc atcttcgatt tcagcagcct tgcctgatcc tcggcgccgg cgagtggaat
      421 gccccgcttt tctcggctca gccggatgtg gcggaggtct ggtcgctgcc gcgacatgtg
      481 cccttcttcc tcagtccggt tttctggcgg gccctgtggt ccctgcgccg ctgccacccg
      541 ggaccgattt acgtctgcga gcggcagccc cggcagctcg cgcgcgtccg acggcttctg
      601 aggctgagcg ggatcgaccc ggctcgatgc cttttcatca ccgacctcgc agcgagccac
      661 gaggagcact gggtcgaccg atttgtgcgc ttcagtgccg gcaccccggc ggcattacgc
      721 gcctcggact atccgctccc gcggctgcag gatgtgccgg cgcccgtgct gtgtgtaacg
      781 gactccgagc ggaccgagcg ggacgcatgg atccgctcgc aaggctggga cggccggccg
      841 ctcatcctcg tgcaacccgg caaccgccgc agcatgagca agcgccgcga acggcggcag
      901 ctcaatgctg atgacaagct ctggcccacc gaaaggtggg catctttgct ggagagaatt
      961 cagggccgca tgcccgaggc gctgatcctt ctgtgcggca ccgcgcggga ggaggccctg
     1021 ctcggggaaa tccgagcggc cgcccgccga gccggtgtgg tggaggcgaa gttgccgctg
     1081 cggccgctgc tggcactctg tgaagttgcg cacagcatga tctcggttga caccgggccg
     1141 gcacacgctg cagccgcgtt gggccttccg ctcgtcgtac tgttcggtgc ggagtcgcca
     1201 cgccgttggt tgccccgcag cgcattgggc tcggcggtga tgggagtcgg aggtccaccg
     1261 gagcgcgtgc gcgcggatca gatctccgtg gaggatgtct tcagcatctg gtgcgagctg
     1321 ctcgccaaaa aggcggcgcc atacaggcac ccggctcgca cgtacgaagg cgcgtgagca
     1381 cggcaaccag ccaggtgcgc acttccctga ttcactctgc caggagacct ccgtgccatc
     1441 gactgctttg aagaacggtt gcccgctcgt gtgcctgtgg tttgcagctg cagcggcatg
     1501 gctgcccgca ccctgcacgc tcgcggccga acccgagccg gtgaagcatg agttcatcat
     1561 caagaacttc aagacggaga gcggcgcggt gctgcccgag gcgaggatcg tctacggcac
     1621 ttatggaaag ctgaacgccg cgggcgacaa cgccatgctg ctgccctcgc attacatggc
     1681 ggaaatgcat ggttatgcgt ggctgattgg ccccggcaag gctctggatc ccaaccggct
     1741 gttcctgatc acgagcgagc tgttcggcaa tggtcgctcg tcctcgccga gcaacactgc
     1801 ggagccgatg cacgggccac gtttccccgt gatgaccatt cgggacaacg tgcacgccgt
     1861 gcacgagctc ctcggatcgc tgcacgtgca tcacctgcgt gccgtcgtgg gcttctccat
     1921 gggcgcgcag caggctttcc agtgggcagt gagttatccg gattacatgg accgtatcgt
     1981 tgcgacctcg ggcacggcaa agacctacgg tcatggaatc gtgcgtctgg aggggcagat
     2041 tgcggccctg accgccgatg cggccttcca gggcggcaac tacagcactc cgcccgaaaa
     2101 gggactgcag gcctttgcag tggtctggac cgggtggctg ttctcgcagg aatggtggcg
     2161 ccgggagctg tggcgcacgg atgcctcgct cggcagcacc ttcccacagg tgctcgagca
     2221 cttccgcacg aaattcatcc cgggcgcgga tgcgaacgac ctgatcctgc agatgcgcac
     2281 gtgggagagc aacgatgtgg gcaatacgcc gccgttcgcg ggcaacgtcg agacggccct
     2341 gcgttcgatc aaggcacagg tcttgtacat gccctcggag accgaccttt actttccgtt
     2401 gagcgatgcg cggtacgagg ccgcattcat tccacactgc tcgctcgtac cgataccctc
     2461 gctgtgggga caccccgccg gtgcgggtgc ctccagggag gatgcgaaat tcctgaacga
     2521 gcatatcggc gccttcctcg agggcgagag cgcgacgaac tgacagcgga tcgattcctc
     2581 ttccatcccg agcatagaat ctcgacatgc aaacacttca cagactccat accggcacgg
     2641 cgatgctcgc cctcgccgcg ctgctcgccc tgcctcaccc ggccgctgcg cgcaccgaaa
     2701 gcgcattcac gatggcggac gtgctggact atccctatac cagcgagctg gttgcggccg
     2761 agcacgcgga cgtgatcgca tgggtgcgca acctgcaagg cgtgcgcaac gtgtgggtgg
     2821 cgaagggtcc ggcctatacg cccatccaag ccactcacta cacgcaggat gatggacagg
     2881 agctcacgca gctcacattc acccccgacg gagaccacat cgtgtacgtg cgcggcggcg
     2941 atcacgatgc caactggccg gccgaaggca acctggcacc cgatcccgac tcctcgaccg
     3001 agcagcccga gacgacgatc tggagcgtgg gcactgacgc cagcgcgccg gtcaagatcg
     3061 ccgaaggcga tactccagcc atctcatcgc ggggccaact tgcttacacg aaggatgatc
     3121 acgtctggac cgtctcgctc gacggtcagg gcaaggccga gcgcctgttc ttcgatcgcg
     3181 gcaaggacag cgatttgcgt tggtcagatg atgggtcgcg gcttgcgttc gtatccgacc
     3241 ggggcgacca cgcgttcata ggcatcttct catcgaagga tcgcccgatc agttacctcg
     3301 ctccatccac cggcaatgac agctccccgc gctgggcgcc ggatggttcg cgtatcgcct
     3361 tcgtgcgaca gcccggcacg ggtggcgcgc ccgagcctct gctgaaggag gtgccgcatc
     3421 cgtggtccat atgggtggcc aacatcgccg atggcgcagg acatctggtg tggcagagcc
     3481 cggacacgct ggcaggctcg tttccagaag tggcggacgg cgcgaatctg tactgggcag
     3541 caggcgatcg gttggtgttt ctggcgtatc tcgacgattg gccgcatctg tattcgaccc
     3601 cggtcgctgg cggcgcaccg gttctgctga cgcccgggtc gtacatggtt gagcatgtcg
     3661 cacagagccg cgatcgtcgt ttcatggtct ttgatgccaa caccggcaaa actcaggacg
     3721 acgacgaccg gcgccacctg atgcgtgtgg ccgtggacca tcccggtcca gccgagctga
     3781 catccgggga cactctcgag tgggctcccg tcaccactgt gagcgatcac atcgcctacg
     3841 tctccgcggg cttccaacag ccgccggcgc cagccgtgat cgagttgaat gggcgccacg
     3901 cgccgcagcg ggtgggcaag gatctgccga cggactttcc gctcaaggca ttcatgacgc
     3961 cacgctcggt gagcttcaaa gcagccgatg gtcttctcgt gcatggtcag ctgttcgatg
     4021 caggggcagg ggcaggggca ggcgcacgcg gcggcggcgg caagccggga gtcattttcg
     4081 tacatggcgg gccgccgcgg cagatgctgc tgggctggca ctacatggac tactacagca
     4141 acgcctacgc agtgaatcag tacctcgccg cgcacggatt cgtggtgctt tcgatcaact
     4201 atcgtctcgg cataggctat ggccgcgcct accatttccc ggagcatgca ggtcctcgcg
     4261 gggcggccga atatcaggac gtcgtagcgg gcgcgcgata tctgcagtct cggccgggcg
     4321 tggatcctgc ccggatcggc atctggggcg gctcgtatgg cggctatctc accgccctgg
     4381 cgctggctcg cgatccggat atcttcaagg cgggcgtgga cctgcacggc gtgcacgact
     4441 ggtcggccct gcccgatatg cctttcagca aagccccgac gcgctacgag aagggcgatc
     4501 gtgaggaggc gatccacatg gcctgggaat cctcacccga tgccagcatc gatgcctgga
     4561 agggacgcgt gctgttgatt cagggcgacg atgatcgcaa cgtcccgttc cagcagacgg
     4621 tggacctggc gaggcgcctg caggcacaca aggtgcccta tgaggagctg gtgatcccca
     4681 acgagatcca cggattcctc agacacgcga cctggctgca cgtggatgag gccactgcgg
     4741 agtttcttgc gcgcaccctg ggcgcgcggc actaggagga aatcccccga ccccccgcaa
     4801 gtctggcaca ctgctgacgg ggagtggacc cgggccggct gggggtgctt tgagcctact
     4861 cgtccgaatc aacgtcgctc ttgccgcaac gttcatcatc gccgctgcgg ctctgggata
     4921 cgtatgttcg accatgttgc aggcgaacgc gaagcaaaac gtgctggagc aggccggtct
     4981 gatgatcgac agcgcgctgg cgactcgcga ctacaccgcc gatgagatcc tgcccttgct
     5041 caatgcacag atgcgaaccg aattcctgcc gcagagcgtg cccttctacg cggccac
//
LOCUS       DASRHN010000122         7634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_864640, whole genome shotgun sequence.
ACCESSION   DASRHN010000122 DASRHN010000000
VERSION     DASRHN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864640"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFB37_04670"
     CDS             <1..429
                     /locus_tag="VFB37_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21777.1"
                     /translation="AQEGKARAASRAELSGRLQQLIDDPQHLASDGAKDEARSLLQTA
                     EAQSPSGPVLRSQVAQLQILLVGEDKPVRLSLLSDNSTQVAISSIGSFGTFSRREIQL
                     RPGKYTVVGTRSGFREVRRVITVAPGQDSQTIRVSCSEPI"
     gene            445..2592
                     /locus_tag="VFB37_04675"
     CDS             445..2592
                     /locus_tag="VFB37_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZO21778.1"
                     /translation="MTHASSYAQVRVREPAGERILGDRPTLGGLGADVVVPGAGTGAA
                     IQFERQDGTWVALSAGDSRVQIDGVPLRGARDLRRQDVLRVGDAQIIVSEVSRTLLRI
                     EVCHLAGNATLPPSGIVSSLADVEGGDEEVEIRTAAPAPGGTTPRRLAPAEARRRRLY
                     AAVALISSLAVLGLISRIVPVALDVEPDDARLRVPGTLLAFHLGGRLFLFPGAYLVRA
                     EHQGYEPAQVAITVRHAGQTALRLHLIKLPGTLQIDTGGIASTVSIDGLESGRAPGDV
                     LVPAGEHTIAIRAPRYVDYIAPVRIEGALTRQNLKITLQPSWGTLQISTIPSGAQISV
                     DGNEAGKAPAVVDAPSGVRRVRISAPGLKTWESSLVLKAGEALNVGPITLGQADAHLV
                     LHSDPSGADVMIGGTYRGRTPLESDLPAGIEHEVTLSLRGYATWTKPVFAESGRRIAM
                     TVPLAVVGGRVAVEGEPDGAQLLIDGIDRGRTPQSLVLSAVEHHIEVHKEGRTPFALN
                     LTPAPGLDRTIRYKLGYVEHALAPDEVARTLYSQTGYLLRLVVAANFAVSGEQTVARR
                     VSGALPPLLSDRPFYVGVAEVTNEQFRRFRPDHVSGSDQRSPGSADQPVTQVSWNEAA
                     AFCNWLSEQDNLPPAYARSEGRYVLRRPVSIGYRLPMQAEWEYAARNAATLGLHDLPG
                     RLSEWVNDDREGAAVAVDAGRAARGFRIVRYAQ"
     gene            2625..2858
                     /locus_tag="VFB37_04680"
     CDS             2625..2858
                     /locus_tag="VFB37_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21779.1"
                     /translation="MRKIWQPIWIMGLISLLFLGAAFLTLRAAEQAIAPATPPSHPPP
                     SAPEESATITDDPTIAPDAAQSADHNVSFPTDI"
     gene            2883..4286
                     /locus_tag="VFB37_04685"
     CDS             2883..4286
                     /locus_tag="VFB37_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZO21780.1"
                     /translation="MRALALELDDRALSLARAGCVLTTAPSAMFDRDSGMLGPHAWSR
                     VRSHPTLTSTRHLGLVLTGRGAATPAPAQVREEIVQLLAAHPPQTEESIWIAIPAQAQ
                     VPGVESLLQIARGASVPLAGFLDAATASVTALGLQRPAIVLEFGLHHAAATAVEVVSG
                     RACRRRALVAANGGLIGLYQAWLGLISATMVKRTRFDPLHDASIEQQLFDVLPELARE
                     AVITGGSTAQVTNGVERFQVELTHDQFVAAAEPVARQLASLVHGLRPAGASLAIVVPR
                     AIADLPGLRSAIDQFVGCELVKLPDGFAAAAASILELPEGPPEQDSVRLVRRLPVQAT
                     PELAARTSREWIGEGRLREPPPSHVLFEGRAYSLEGPPLIVGRTAAGSELQPAAGAQI
                     TITLPEGLAGVSRRHCTLARDSGQVLLLDHSRFGTFVNGERVAERVHLHAGDRVRLGD
                     PGVELELIAMSDKTVTG"
     gene            4302..5402
                     /locus_tag="VFB37_04690"
     CDS             4302..5402
                     /locus_tag="VFB37_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZO21781.1"
                     /translation="MTARRRTVEVFTLSFLDCICCGFGAVILFYTIVSAQAGMQSYRT
                     ADDLHAEVNRLEEEVRGGSRGLATLRNEVLQTESETASDEARTRSIAAAIASRREQIA
                     AHDADTLAQQQRIAKLKADVRSLQEGRKRLEAGSLDRTPPGQQVNSFRDVGGDRHYIT
                     GIRMRGQHILFLVDASASMLHEDLVSILRLRNADDAQKRAAAKWRRAVDTVSWLATQV
                     PPTSDYQIYTFNTRAQPLSVDSGGAWIPAADVAELARDLAQLRNIVPAEGTSLYNAFA
                     AARTLSPLPDQIVLITDGLPTQGRTASSRKYVDSAARMRLFDEALAEVPNGVPVDSIL
                     LPMQGDLQAAHRFWNLARQSHGTLLMPSKDWP"
     gene            5399..6385
                     /locus_tag="VFB37_04695"
     CDS             5399..6385
                     /locus_tag="VFB37_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21782.1"
                     /translation="MRGGRRRSVEVFSLSFLDCICCGFGAIILLLVMTQFARPARLED
                     SRRDLQAQVRDLQQQLHVIRGESDDLERQLTGRIDRRERERQRLAHLSGDLSSIQGQY
                     SASQESAALTNNVVRELVANRQKLSAEMLRLLQEQVRPTTEAIGGIPIDSEYVIFVVD
                     TSSSMTTAHWDANLAIIDEILGMYPHVRGLQVMNDQGIYMFAGTKGRWLEDSPAERAQ
                     IRAQARHWHAFSQSNPVPGMEEALRTYWSADRRVSLFVLGDEFTGQSIQTALDAIGNL
                     NKPDASGRRLMRIHAIGFAEAPGMSPYTSIRFSALMRLVCEQNNGTFVGLRQ"
     gene            6492..7211
                     /locus_tag="VFB37_04700"
     CDS             6492..7211
                     /locus_tag="VFB37_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZO21783.1"
                     /translation="MADGGPRLVMLAGATGFVGGFALESLLDASDVARVFAVTRRPLG
                     REHPRLANRIVQFDSIESQLKGLSCHAALCCLGTTIRQAGSQEAFRKVDLECVLAFAR
                     TAKAAQAQRFVVVSSVAADPGSRNFYLRTKGEMEQALAALGFASLEILQPGVLLGWRH
                     AMRPLELAARLAMPLINPFLIGEREQYRGIPARTVGEAMAGVLRSSRRGVQRYTYAGI
                     QGLARLKPARALAKAASSPEK"
     gene            7335..>7634
                     /locus_tag="VFB37_04705"
     CDS             7335..>7634
                     /locus_tag="VFB37_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21784.1"
                     /translation="MTRMGRRMAAVVALCAALPLILLALAAARQADTAGDALITSRLT
                     DASTLYADLIKSRLGVAETIVEAITANDVGSERSTLERQAGNSRAFKSVVITDSEG"
BASE COUNT         1301 a         2458 c         2557 g         1318 t
ORIGIN      
        1 gcccaggagg gcaaagcaag agctgcttcg cgcgccgagc tgtccggaag gctgcagcag
       61 ctcatcgatg acccgcagca cctcgcctct gacggggcaa aggatgaggc ccgctcgctg
      121 ctgcagaccg cggaggcgca gtcgccatcc ggtccggtgc tgcgctctca ggtcgcacag
      181 ctgcagattc tgctcgtcgg ggaggacaag cccgtgcgcc tgtcactgct gtccgataac
      241 agcacccagg tggccatctc cagcatcggc tccttcggca ccttttcccg gcgcgagatt
      301 cagctgcgcc ccggtaagta caccgtggtc ggtactcgca gcggatttcg cgaggtacgc
      361 cgcgtgatca cggtcgcgcc cggacaggat tctcaaacca tcagggtcag ttgctcggaa
      421 ccgatctgac ttcgccacac tgcagtgacg cacgccagca gctatgcgca ggtgcgcgtc
      481 cgcgaacccg cgggcgagcg catcctcggt gatagaccga cgctgggcgg acttggggcc
      541 gatgtcgtcg ttccaggtgc aggcactggg gcggcgatcc agttcgagcg tcaggacggc
      601 acgtgggttg ccctgtcagc cggcgacagc cgggtgcaga tcgatggagt ccctctgcga
      661 ggcgcccgcg atctgcgtcg tcaggatgtg ctgagggttg gcgatgccca gatcatcgtc
      721 tcggaggtct cccgaactct gctccgcatc gaggtatgtc acctcgcggg taatgcaact
      781 ctgcctccct ctggaatcgt gtcctcgctc gccgatgtgg agggtggcga cgaagaagtg
      841 gagattcgca cggccgcgcc cgctcccggc ggcacgaccc cgaggcgatt ggcgccggcc
      901 gaggctcgaa ggcgcaggct ttacgccgct gtcgccctca tctcgtctct ggcggtgctc
      961 ggtctcatct cgcgaatcgt gccggtcgca ctggatgtag agcctgatga tgcgaggctt
     1021 cgggttccag gcacgctgct cgcctttcat ctcggcggcc ggttgtttct ctttcccgga
     1081 gcatacctgg taagagccga gcatcagggc tatgagccgg cgcaggtggc aatcaccgtg
     1141 cgccacgcgg ggcaaaccgc cttgcgactc catctgatca agctgcccgg gacactccag
     1201 atcgacaccg gcggcattgc ctccacggtc agcatcgacg gcctcgagtc cggccgtgca
     1261 cccggagacg tgttggtgcc cgcgggcgag cacacgattg cgatccgggc gcctcgatat
     1321 gtggattaca ttgctcccgt ccggatcgaa ggagccctca cccgccagaa cctgaagatc
     1381 accctgcagc cctcgtgggg gacactgcag atctcgacca ttccctcagg agcgcagatc
     1441 agtgtagacg gcaatgaggc aggtaaggct ccggcagtgg tcgatgcccc atccggcgtc
     1501 cggcgagtgc gcatttccgc tcccgggctc aagacatggg agagcagcct cgtgctcaaa
     1561 gccggtgaag cgctcaacgt cggccccatc acactcgggc aggcggacgc tcatctggtg
     1621 ctgcattcgg atccgagcgg ggcagatgtc atgatcggag ggacctaccg cgggcgcaca
     1681 ccgctggaga gcgacttgcc cgccggcatc gagcacgagg ttaccctcag cctgcgcgga
     1741 tatgcgacct ggacgaagcc ggtgtttgca gagtcgggcc ggagaattgc gatgaccgtt
     1801 cccctggcgg tcgtcggcgg ccgcgtcgcc gtggagggtg aaccggacgg agcgcaactg
     1861 ctgatcgacg gcatcgatcg cggtcggacg ccgcaatctc tggtgctctc tgccgtggag
     1921 caccacatag aagtccacaa ggaaggccgg accccattcg cgctgaacct gactccggcg
     1981 cccggcctgg atcgtacgat tcgttacaaa ctcggatatg tcgagcacgc actggcaccc
     2041 gacgaagtgg caaggaccct gtactcgcag acgggctacc tgttgcgact ggtggtcgca
     2101 gcgaacttcg cggtgagcgg cgagcagacc gtagcgcgcc gggtgagcgg agctttgcct
     2161 ccgctgctgt ctgaccggcc gttctacgtt ggagtggccg aggtcaccaa tgagcagttc
     2221 cggcgctttc gtcccgacca cgtctccgga tcggaccaac gctcgccggg ttcagcggat
     2281 caacccgtga cacaggtgag ctggaatgaa gcagccgcct tctgcaactg gctttccgag
     2341 caggacaatc tcccgccagc ctacgcacgg agcgagggac ggtacgtgct gcgccggccc
     2401 gtcagcatag gctatcgcct gcccatgcaa gcagaatggg aatatgccgc ccgcaacgcc
     2461 gccacactcg ggctgcacga tttgccgggg agactgtctg agtgggtcaa cgatgaccgc
     2521 gagggggcag ccgtcgcggt cgatgccgga agggcagcgc gcggtttccg catcgtgcgc
     2581 tacgcgcagt gagccggccg ggaccggcac gaggagtgtg actcatgagg aaaatctggc
     2641 aacccatctg gatcatgggg ctgatctcgc tgctgttcct gggagcggct ttcctcacgc
     2701 tgcgggcggc agagcaggcg attgcaccgg ccacgccgcc ctcgcacccg cctccctccg
     2761 cgcccgagga gtcagcgacg atcaccgatg atcccacgat tgcgcccgat gcagcgcagt
     2821 cagcggatca caacgtcagc tttccaaccg acatctgagg cgcggaccgc tcgccggcgc
     2881 agatgagggc actcgctctg gagctcgatg atcgtgcgct gtcgctggcg cgagccgggt
     2941 gcgtgctcac gactgcgccg agtgcgatgt tcgatcgcga ttcgggcatg ttaggtccgc
     3001 acgcgtggag ccgcgttcgc tcccatccga cgctcacctc aacgcgccac ctgggactgg
     3061 tgttgacggg ccgcggcgcg gcgactccgg ccccggcgca agttcgcgag gagatcgtcc
     3121 aactgctcgc ggctcatccg ccgcagaccg aagagtccat ctggattgca atacccgccc
     3181 aggcgcaggt gcctggggtg gaaagcctgc tgcagatcgc gcgcggcgca tcagtgccct
     3241 tggcggggtt tctggatgca gcgacggcca gcgtcacggc cttgggcctg cagcggccgg
     3301 cgatcgtgct ggaattcggg ctgcaccatg ctgcggccac cgcagtcgag gtggtgtcgg
     3361 ggcgggcgtg ccggcgtcgc gcgctggttg ccgccaacgg cggactgatc gggctgtacc
     3421 aggcatggct gggcctcatc agcgcgacca tggtgaagcg cacacgcttc gatccattgc
     3481 acgatgccag catcgagcag caactcttcg atgtgttgcc ggagcttgcg cgtgaggctg
     3541 tgatcacggg cggctcgacg gcgcaggtca ctaacggcgt agagcgcttc caggtcgagt
     3601 tgacacacga ccagttcgtg gcggctgccg agccggttgc ccgacagctc gcgagtctgg
     3661 tgcatggctt gcgtccggcg ggcgcttcgc tggcaatcgt cgttccgcgg gccatagctg
     3721 atctgcccgg cctgcgcagt gcgatcgacc agttcgtcgg atgcgagctc gtgaagctgc
     3781 ctgacgggtt cgctgcagcc gccgcctcaa tcctcgagtt gccagagggt ccgcctgagc
     3841 aggacagcgt gcgcctggtg cgcaggctgc ctgtgcaggc gacgccggaa ttggccgcga
     3901 ggacatcgcg cgagtggatc ggcgaagggc gtttgcgcga accgccgcca tcgcacgttc
     3961 tgttcgaggg ccgtgcctat tcactcgaag gtccgccgct gattgtcggc cgcaccgcgg
     4021 ccggctcgga actgcagccc gcagcaggtg ctcaaataac aatcaccctg ccggagggac
     4081 ttgcgggcgt gtcgcggcga cactgcacgc tggcgcgcga cagcgggcag gtgctgttgc
     4141 tcgatcacag ccggttcggc acgttcgtca atggtgagcg cgtggcggag cgtgtgcacc
     4201 tgcacgccgg cgacagggtt cgactgggtg acccgggagt cgagctcgag ctgatcgcca
     4261 tgagcgacaa gacagtgacc gggtgagaga gcggtaactg catgaccgcc cgacgccgca
     4321 ccgtcgaagt cttcacgctg tcgtttctcg actgcatctg ctgcggcttc ggggccgtaa
     4381 ttctcttcta caccatagtg agtgcgcagg cgggcatgca gagctatcgc acggccgatg
     4441 atctgcatgc ggaggtcaac cggctcgaag aagaagtgcg cggcggctct cgcgggcttg
     4501 cgacgctgcg gaacgaggta ctgcagaccg agagcgagac cgcaagcgat gaggcgcgca
     4561 cccggagtat cgccgccgcg atcgccagcc gccgcgagca gattgccgct catgatgcgg
     4621 acacgctcgc gcagcagcag cgaatcgcga aactgaaggc agacgtgcgt tcgctgcagg
     4681 aaggcaggaa gcggctcgaa gcgggcagcc tcgatcgcac cccgccaggt caacaggtca
     4741 acagcttccg ggatgtcggc ggcgatcggc actacatcac cgggatccgg atgcgcggcc
     4801 agcacatcct gtttttggtc gatgcctcgg ccagcatgct gcacgaggac ctcgtgagca
     4861 ttctgcggct gcgtaatgcg gatgacgctc agaagcgtgc agccgccaaa tggcgtcgcg
     4921 cggtcgacac agtgagctgg ctcgcgaccc aggtgccgcc cacgagcgac tatcagatct
     4981 atacgttcaa cacgcgagcg caaccgctgt cggtggattc gggcggagca tggatacccg
     5041 ccgcggacgt ggccgagctg gcccgggacc tcgctcagct gcgcaacatc gtgcctgccg
     5101 aaggcacgag tctctacaat gcattcgccg ctgcgcgcac gctcagcccg ctgccggatc
     5161 agatcgtgct catcaccgac ggtctgccta cccagggaag aacggccagc agccgcaagt
     5221 atgtcgactc ggccgcgcgc atgcggctgt tcgatgaggc actcgccgag gtgccgaatg
     5281 gcgtgcccgt ggactcgatc ctcttgccga tgcagggcga tctgcaggcg gcgcatcgct
     5341 tctggaacct ggcccgtcag tcccacggca cgctgctcat gccttcgaag gactggccat
     5401 gagaggcggg cggcgccgca gcgtagaagt cttcagcctg tcgttccttg actgcatctg
     5461 ctgtggcttc ggcgccatca tcctgcttct ggtgatgaca cagttcgcgc gcccggcccg
     5521 tctggaggac agccgccgtg acctgcaggc acaggtgcgc gacctgcagc agcagctcca
     5581 tgtcattcgc ggggagtctg atgacctgga acgccagctc acagggcgca tcgatcggcg
     5641 cgagcgcgag cggcagagac tggcccacct gtcgggcgac ctcagcagca tccagggaca
     5701 gtattcggcc agccaggaga gcgccgccct caccaacaac gtggtgcggg agctcgtggc
     5761 caaccgccag aagctcagtg cggagatgct gcgcctgctg caggagcagg tccgtccgac
     5821 aaccgaggcg atcggtggca ttcccatcga cagcgagtac gtgattttcg tggtggacac
     5881 ctcctcgagc atgaccacgg ctcattggga tgcgaacctg gccatcatcg atgagatcct
     5941 gggtatgtat ccgcatgtca ggggcctgca ggtgatgaat gatcagggca tctacatgtt
     6001 tgccggtacc aaaggccgct ggctcgagga ctctccagcc gagcgcgcgc agattcgtgc
     6061 gcaggcacgc cactggcacg cattcagcca gtcgaaccct gtgccgggaa tggaggaagc
     6121 tctgcgcacc tactggtcgg cagaccggcg tgtcagcctg ttcgtgctcg gcgatgagtt
     6181 cacgggtcaa tcgattcaga cggcgctcga tgcgatcggc aatctgaaca agcctgatgc
     6241 cagcggccgt cgcctgatgc gcatccacgc catcggcttt gcggaggcgc caggcatgtc
     6301 gccctacacg agcattcgtt tctccgcgct catgcgcctg gtctgcgagc agaacaacgg
     6361 caccttcgtc ggcctgcgcc agtaggtgcc gcgcagcggc gcaccggtcc gtgggtggcg
     6421 aagcgcgggg gacccggtta agctctgccg cgcccagacc aacatctcac gacttccgag
     6481 cgaatgtgcg catggccgac ggcggaccga ggctggtgat gcttgccgga gccaccggct
     6541 tcgtgggagg ttttgcactc gaatcgctgc tggacgcatc cgacgtcgcg cgggtgttcg
     6601 cagtgacccg ccgccctctc gggcgggagc atccgcggct tgccaaccgc atcgtacagt
     6661 tcgacagcat cgagtcacag ctcaagggac tgagctgcca tgcggcgctc tgctgtctgg
     6721 gcaccacgat ccggcaggcc ggttcccagg aagctttccg caaggtggat ctggagtgcg
     6781 tcctggcttt cgcgcgtacg gccaaggcgg cgcaggcgca gcgcttcgtg gtggtctcct
     6841 cggtcgctgc agacccgggc tcccggaact tctacctgcg gaccaagggt gaaatggagc
     6901 aggcgctcgc ggctctcggg tttgcctcac tcgagatcct gcagccgggc gtgctgctgg
     6961 gttggcggca tgcgatgcgg ccgctggagc tggcggcgcg gctggcgatg cccctgatca
     7021 atccctttct catcggggag cgcgagcagt atcgcgggat tccggcgcgg actgtcgggg
     7081 aggcgatggc gggggtgcta cgctcgagcc gtcgcggcgt gcagcgctac acctacgcag
     7141 gaattcaggg gctggcgcgt ctgaaacccg cccgtgcgct cgccaaagct gccagctcgc
     7201 cggaaaagtg accgggttca cgcggcggat tttccaataa ttgacgcaat cgccttccgg
     7261 cgttgccacc ggatggatac attccgccgc gactctaccg cgcggtagct actggaatgg
     7321 atcggaccgc gatcatgacc cgcatggggc ggcgcatggc agccgtcgtg gcgctgtgcg
     7381 cagccctacc cttgattctt cttgcgcttg cggccgctcg tcaggcggat accgcgggcg
     7441 atgccctgat caccagtcgg ctcacggatg ccagcacact ctatgcggac ctgatcaagt
     7501 cgcgtctcgg cgtggcggag acgatcgtcg aggccattac ggccaatgac gtaggctccg
     7561 agaggtctac gctcgaaaga caagccggca actcccgcgc cttcaagtcg gtcgtgatca
     7621 ccgacagcga aggg
//
LOCUS       DASRHN010000123         8494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1521972, whole genome shotgun sequence.
ACCESSION   DASRHN010000123 DASRHN010000000
VERSION     DASRHN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521972"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..778)
                     /locus_tag="VFB37_04710"
     CDS             complement(98..778)
                     /locus_tag="VFB37_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21785.1"
                     /translation="MRILLVEDEAKLCELIARALRAERYAVDTAGDGTAGWELADAYE
                     YDLIILDLMLPSLSGTEILRRVRRKNQTVPILVLTARDATEEKVQNFEAGADDYLTKP
                     FAFAELLMRVKALLRRGTASRANVLRVADLEVDRLSQNVRRAGKRIELTPKEYALLEY
                     LAANPGRVFSRTMIIEHVWDQSFEGLTNIVDVYVRHLRTKVDDPFPTKLIRTVRGVGY
                     GLSEGGDS"
     gene            1417..2814
                     /locus_tag="VFB37_04715"
     CDS             1417..2814
                     /locus_tag="VFB37_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZO21786.1"
                     /translation="MTSRTPLQSARSTPALATAAAASLLGTLSGVAQAQTVPADDALT
                     MHGVTLYGIVDIGLQCDSHGAPFNDYFPAGSNDIVQKDSNHGVCGATPSNLSQSRVGL
                     QGKEPLMGDWSAVFRLETFFNPQSGEISDALRSLVQNNGRSAKEQTVNLDSSVAGQAF
                     QQSYVGVSSPSFGTLTFGRQNTLLADAIAKYDPQAASQAFALIGLSGTTAGAGDTQDR
                     RLDSSLKYSLNSTNGLHAGAMYKFNGSTGAANTAFEGQLGGEFAGFSVDGYYVHVRDA
                     IAASALSATQLSTLAAGFSPSNSLSGTVSDNTSFTIAGDYKLGVVTLYAAYEHIKFAN
                     PDTPLDAGFDDIGGYKLAFVNNTAFPHDKILQVFWAGVKFNPTPMIELNAAYYGYKQN
                     SFAGGANTGCSDTRNAGCSGTENAVSFSAVYKATKRFDLYAGAMWTDVTNGLASGFDF
                     SRSNIDPTIGVRFKF"
     gene            2904..3110
                     /locus_tag="VFB37_04720"
     CDS             2904..3110
                     /locus_tag="VFB37_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HZO21787.1"
                     /translation="MSDSQSKRGRWSWWYLLFFLQFIAVLWPPFYNKLDPEWLGMPFF
                     YWYQLLWVVISAVLTGIVYWATET"
     gene            3158..4705
                     /locus_tag="VFB37_04725"
     CDS             3158..4705
                     /locus_tag="VFB37_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZO21788.1"
                     /translation="MHDVNWVALVIFVLLFALITWLGFAAAHWRKGDLEQLHEWGLAG
                     RRFGTIVTWFLVGGDVYTAYTFIAVPALAFGTGAVAFFAVPYTIVVYPILFIVFPRLW
                     YVCNKHGYITSGDFVRGRFGNRWLALAVTITGIVATMPYIALQLVGIQVVVGALGISG
                     SGLAADLPLIIAFLVLAAFTFTSGLRAPASIAIVKDTLIYLTALVTIIAVPIQLGGFH
                     HIFSTVPATKLLLAAPGPHTTGSYGSYATLALGSALALFLYPHSLTGILSANSGHAIR
                     RNAAILPGYSLMLGLLAMFGFFAIAANVQHMPEYAGGFKLFGNNFAVPALLLHSFPSW
                     FVGVAFAAIAIGALVPAAIMSIAAANLYTRNIHREFINPNPTDQQEARMAKWVSMIVK
                     LGALVFILFVPTEYAIQLQLLGGIWIIQTLPAVMLGVFTRWFNSWALLVGWIVGTFAG
                     TAMAVAAHLKPTFPLEIGGYTFPGYTAFYTVILNLVLAALLTPIFNMARAGRESVDAT
                     AAADYQA"
     gene            4919..5647
                     /locus_tag="VFB37_04730"
     CDS             4919..5647
                     /locus_tag="VFB37_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21789.1"
                     /translation="MRAARFMSRLPVLIITALLLGTAGIATAAGGPGGGGGGGGHGGG
                     GGGGHGGGGGHGGAASHGFGGAHGASGHGYGHGYGHGWGHRGGHGPGHWRGGGWGWGF
                     WGGVGLGWYVPFLPWGYETYWWDGEPYYYAGGNYYAWDDSAGEYEAVEPPDGQPQSNP
                     TEPAAGAANPGVSFELYAYPKAGQSDEQQARDRDECRKWASQQSGFDPAKPADNPASD
                     DMTKRQSYLRAEAACLEARNYTVR"
     gene            complement(5664..6359)
                     /gene="phoU"
                     /locus_tag="VFB37_04735"
     CDS             complement(5664..6359)
                     /gene="phoU"
                     /locus_tag="VFB37_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZO21790.1"
                     /translation="MLEGHISKAFDGALAGLHIRVVEMGGLVLEQVREAAYAYTEWNA
                     AAAERVLQRETQVSAYDTSLDEEQLTLLARRQPVAGDLRATIAMSKSVAELERAGHEA
                     KKIARVVLAQDSRPGVATSRDVRHLGQLAVSLLRLSLEAFDRLDPTLALDVIARDDEL
                     DLEYAAGLRRLLTRAMEDPRRFDVALEAAFVLKSLERVGDHARNLARHVRSMTGETPS
                     ADQHAAPRASPLS"
     gene            complement(6377..7321)
                     /gene="pstB"
                     /locus_tag="VFB37_04740"
     CDS             complement(6377..7321)
                     /gene="pstB"
                     /locus_tag="VFB37_04740"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZO21791.1"
                     /translation="MNDVHNDVTQERRAMGASGIPEEPHRRRIGEGGLNLKPSGRTPA
                     AASLEQRAGEQTVGQASVADAVFTCRDVNVFYGSKHALRSVSIDVARRQVLAMIGPSG
                     CGKSTFLRCLNRMNDTIPGARVTGSIHLDKTDIHDKRQDVVQLRARVGMVFQKPNPFP
                     KSIYENVAYGPRIHGIPRSRADLDEIVCTSLQRAGLWDEVKDRLNRPGTGLSGGQQQR
                     LCIARAIAVQPEVILMDEPCSALDPVATARVEDLIEQLRASYAIVIVTHSMQQAARVS
                     QRTAYFHMGSLIEVGETDRVFTNPRHRLTEDYITGRFG"
     gene            complement(7328..8134)
                     /gene="pstB"
                     /locus_tag="VFB37_04745"
     CDS             complement(7328..8134)
                     /gene="pstB"
                     /locus_tag="VFB37_04745"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZO21792.1"
                     /translation="MSASAASVRANGHSLEAHAPKFEVRHLDFYYGQNRALKDISLSL
                     ADRSITAFIGPSGCGKSTLLRVFNRMYALYPGHYATGEVLLDGLNILAPTVDLSALRF
                     RVGMVFQKATPFPMSIFNNVAFGLRLARRIPRSELEKRVEVALRDAAMWDEVKDKLHE
                     DGRGLSGGQQQRLCIARTIALEPEVVLLDEPTAALDPISTLRVEETLQSLREKYCLAV
                     VTHNLQQAARVSNVTAFMYMGELVEVGATEQLFTAPRDRRTDDYVTGRFG"
     gene            complement(8131..>8494)
                     /locus_tag="VFB37_04750"
     CDS             complement(8131..>8494)
                     /locus_tag="VFB37_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO21793.1"
                     /translation="GMKDASTAMGAYPWRTITSIIYPAARSGIVTGLLLAIARISGET
                     APLLFTALNNQFWSTNLRAPIANLPVIIFQFALSPYKNWQQLAWSGALLITFAILVLS
                     VAARIVVSALNPTGSRQR"
BASE COUNT         1503 a         2800 c         2642 g         1549 t
ORIGIN      
        1 cgcagaaaga cgagggtcaa ggcgccgagc accacgaaga ctatcgtgag caggcccgca
       61 taccaggcga cgaggcgaaa gctcagggat cgtgtgttca cgaatcccct ccttccgaga
      121 ggccatagcc cactccgcgc accgtgcgaa tcaacttcgt cgggaacggg tcatccactt
      181 tggtgcgcag atggcgcacg tacacgtcca cgatgttcgt caggccttcg aaactctggt
      241 cccagacgtg ctcgatgatc atcgtgcgcg agaagacgcg accggggttt gccgcaagat
      301 actccagaag cgcgtattcc ttcggcgtga gctcgattcg cttgccggcg cgccgcacgt
      361 tctgcgacag ccggtccacc tcgaggtcgg ccactcgaag cacattcgcg cggctggcgg
      421 tgcctcggcg cagcagtgcc ttgacgcgca tcagcagctc ggcaaaggcg aacggtttgg
      481 tgaggtaatc gtccgcaccc gcctcgaagt tctgcacctt ctcctcggtg gcatcccgtg
      541 cggtcaggac gagaatcgga accgtctggt tcttccggcg aacccgccgc aggatctcgg
      601 tgccgctcag actggggagc atcagatcga ggatgatcag gtcatattca taggcatcgg
      661 ctagctccca gccggccgtg ccatcgccag ccgtatccac ggcgtaacgc tcggctctca
      721 gcgcccgggc gatgagctcg cacagcttgg cctcgtcttc gactaacagg atgcgcatgg
      781 gggtggagtt tccgccgcac aggaaaacac ggccgcatta agcggcggcc aatgcgccat
      841 taaaatccgt tcagaaacgc tgccgagcaa agcaaatgcc gctccggccg gccggaaatg
      901 aggcgtgcgt caccatccaa ttcaacgcct taaccttgag aatacggctg ccggccttgc
      961 ctcgccacgc ccttcccccc agcctgcagt gctcggccgc tgcctcgcga cccgttgatc
     1021 cgcctgaccc ccggacgttc cgtccctccg ctcccggtgc tctcgaggcg cgctcgcccg
     1081 cccccccgcg cctgtcgttg ctcagacgca acaaacccat ccggatcggt gcgtaaacca
     1141 aggccgcacc tgccgcagcg cacccaaacc aacgcgggac gaggtgttgg cccggatccg
     1201 gtacacctcc accctctcgg gcggcccgca acaaaactgt caattatctg aaggccagat
     1261 gtcataatag tgtcgcacag tgacggcccg tcgatgcgtg gatagcctac ggatccagcg
     1321 tatcccggat gtcgcgccat cggggagcgg tggagtcgat cgagccgctg tccgcgcgcc
     1381 gcgtttgcct cttcaaaaaa cataggaacc ttgaagatga catcacgtac tccgctgcaa
     1441 tcggctcgct ccacaccggc cctcgccacc gccgcagccg ccagcctcct ggggaccctc
     1501 tcgggtgtcg cgcaggcgca aaccgtgccc gccgatgatg cgctgacgat gcacggtgtc
     1561 accctgtacg gcatcgtcga catcggcctg cagtgcgact cgcatggcgc gcccttcaac
     1621 gactacttcc cggccggaag taacgacatc gtgcagaagg acagcaatca tggggtctgc
     1681 ggcgccaccc ccagcaacct gagccagtcg cgcgtcggcc tgcagggcaa ggagcccctg
     1741 atgggcgact ggtccgccgt gttcaggctg gagacattct tcaatccgca atccggcgag
     1801 atcagcgatg cgctgaggtc gctcgtgcag aacaacggcc gctcggcgaa ggagcagacc
     1861 gtcaacctgg attcgagcgt ggcgggacag gccttccagc agtcctatgt cggagtgagc
     1921 tcgccgagct tcggcacgct gaccttcggc cgccagaaca cgctgctggc cgatgcgatc
     1981 gccaagtacg acccgcaggc cgcctcacag gccttcgccc tgatcggtct gtcgggcacg
     2041 acggccggcg cgggcgacac gcaggaccgc cgcctggatt cgtccctgaa gtattcgttg
     2101 aattccacca acggcctgca tgccggggcc atgtacaagt tcaacggctc gaccggggca
     2161 gcgaatactg cgttcgaggg ccagctcggc ggcgagttcg ccggcttctc ggtggatggc
     2221 tactacgtgc atgtgcgcga tgccatcgcg gcatccgccc tcagcgcaac tcagctctcc
     2281 acgttggccg ccgggttctc gccgagcaat tcactctcgg gcacggtctc cgacaatacc
     2341 tcgttcacca tcgccggcga ctacaaactc ggtgttgtga ccctgtacgc cgcatacgag
     2401 cacatcaagt tcgcaaatcc cgacacgcca ctggatgcag gcttcgatga catcggcggc
     2461 tacaagctgg ccttcgtcaa caatacggcc ttcccgcacg acaagatcct gcaggtgttc
     2521 tgggccggcg tgaaattcaa cccgacaccg atgatcgagc tgaacgcagc ctactatggc
     2581 tacaagcaga acagcttcgc gggcggagcg aatactggct gcagcgacac tcgcaatgct
     2641 ggctgcagtg gcacggagaa tgcggtcagc ttctcagcgg tctacaaggc caccaagcgc
     2701 ttcgacctct acgccggtgc gatgtggacg gacgtgacga acggcctcgc cagcggcttc
     2761 gatttcagcc gttccaatat cgatccgacg attggtgtac gcttcaagtt ctgagccgcc
     2821 gcggcaagcc gcagcggctt gctgaagaac aacaggctgg gactacgccg cccgggccag
     2881 ccgggcggcg ggggattgcg gcgatgagcg actcacagag caaacgcggt cggtggagct
     2941 ggtggtacct gctcttcttc ctccagttca tcgccgtcct ctggcccccc ttctataaca
     3001 aactcgaccc cgaatggctg gggatgccgt ttttctactg gtatcagctg ctgtgggttg
     3061 tgatcagcgc cgttctgacc ggcatcgtct attgggccac cgaaacctga gcgacggacg
     3121 agcgcagaag aattaagccg aatcccgggg gaatgccgtg cacgatgtga actgggttgc
     3181 tctggtcatc ttcgttctcc tgttcgcgct catcacctgg ctcggcttcg cggccgcgca
     3241 ctggcgcaag ggcgatctgg agcagctgca cgagtgggga ctcgccgggc ggcgctttgg
     3301 cacgatcgtg acctggtttc tggtgggcgg tgatgtgtac accgcctaca ccttcatcgc
     3361 ggtgccggcg ctggccttcg gcaccggtgc agtggccttc ttcgctgtcc cctacacgat
     3421 cgtcgtttac ccgatcctgt ttatcgtgtt cccgcgcctc tggtacgtct gcaacaagca
     3481 cggctacatc acctcgggcg acttcgtgcg aggacgtttc ggcaatcgct ggctggcgct
     3541 cgccgtcacg atcaccggaa tcgtggccac gatgccctac atcgccctgc agctcgtcgg
     3601 catccaggtc gtcgtcgggg ccctggggat ctcgggaagc gggctcgcag cggatctgcc
     3661 actgatcatt gccttcctgg tcctggcggc cttcaccttc accagcggac tgcgcgcgcc
     3721 ggcatccatc gcgatcgtca aggacaccct gatctatctg accgcgctgg tcacgatcat
     3781 tgcggtcccg atccagctcg gcggatttca tcacatcttc tcgaccgtcc cggccacgaa
     3841 gctgctgctg gcggctcccg gcccacacac gacgggctct tacggctcct acgcgactct
     3901 cgccctcggc tcggcgctgg cgctattcct gtatccgcat tcgctcaccg gcatcctgag
     3961 cgcgaacagc gggcatgcca tccggcgcaa tgcggcaatt cttccagggt attcgctcat
     4021 gctcggcctg ctggcgatgt tcggcttctt cgccatcgcc gccaacgtgc agcacatgcc
     4081 cgagtatgcc ggcgggttca agctgttcgg caacaacttc gccgtccccg cgctgctgct
     4141 gcacagcttt ccgtcctggt tcgtcggggt ggccttcgca gccatcgcaa tcggcgccct
     4201 ggtgccggct gcgatcatgt cgatcgcggc ggccaacctt tacacacgca acatccatcg
     4261 cgagttcatc aatccgaatc ccaccgacca gcaggaagca cgcatggcga agtgggtctc
     4321 catgatcgtg aagctcggag cgctggtgtt cattctcttc gtgcccaccg agtatgcaat
     4381 ccaacttcag ctcctcggcg gcatctggat catccagacc ctgccggcgg tgatgctcgg
     4441 agtcttcacg cgctggttca actcgtgggc gctgctggtg ggctggatag tcggaacgtt
     4501 cgccggcact gcgatggcgg ttgcagctca cctgaagccg acgtttccgc tggaaatcgg
     4561 cggctatacg ttcccaggct acacagcctt ctacacggtc attctcaacc tggtgctggc
     4621 tgcgctcctg acgccgatct tcaacatggc ccgtgccggg cgcgaatccg tcgatgcgac
     4681 cgcagctgcg gattatcagg cgtaggagcg gtgtgagttt gcgcctcgca gattaatacc
     4741 ggatgaatac aacattaacg ccgggaacag cgaagagtta gtaactgaaa gcgacgaagc
     4801 gtaggattca atgaccggcg gccatcgtcg agtgcggagt tccgcaagct tgcgagtggc
     4861 gccgtgcggt ccgtcccgag ccccacatgc gggtgcccgg aacggccggg agaatgccat
     4921 gcgcgccgcg agattcatgt cccgactgcc agttctgatc atcaccgccc tcctgctggg
     4981 aactgcgggt atcgccactg cggcaggtgg gccgggaggc ggcgggggcg gaggtgggca
     5041 cggtggcgga ggcgggggcg gtcacggggg cggtggcggt cacgggggtg cggccagtca
     5101 tggcttcggc ggcgcccatg gcgcctccgg acacggctac gggcatggct acggacatgg
     5161 ctggggccat cgtggcgggc acggacccgg acactggcgc gggggcggct ggggctgggg
     5221 cttttggggc ggcgtcggtc tcggctggta tgtgcccttc ctgccctggg gctacgagac
     5281 ctactggtgg gacggcgagc cttactacta cgcaggcggc aactattacg cctgggatga
     5341 ttcggccggc gagtacgaag ccgttgagcc gccggacgga cagccccagt ccaatccgac
     5401 agaacctgcg gcgggcgccg ccaatccagg cgtgagcttc gagctgtacg cgtaccccaa
     5461 ggccggccaa tcggatgagc agcaggcgcg cgatcgggac gagtgccgca agtgggcctc
     5521 gcagcaatcc ggattcgatc cagccaagcc ggctgacaac cccgccagcg atgacatgac
     5581 caaacggcag tcctacctgc gcgccgaggc ggcctgtctc gaagctcgca actacaccgt
     5641 acgctgagag ctcacccagc gtgtcagctc aggggtgaag cacgcggcgc agcgtgctga
     5701 tccgcgctcg gtgtctctcc agtcatgctg cgtacgtgcc gtgcaagatt gcgcgcgtga
     5761 tcgccgacgc gctcgagcga tttcagcacg aaggcggctt cgagcgcgac atcgaaccgc
     5821 cgcgggtctt ccatcgccct cgtcaggagc cggcgcagac ccgcggcgta ctccagatcg
     5881 agctcgtcat cccgcgcgat cacatccagt gccagagtcg gatccaggcg gtcgaaggcc
     5941 tcgagcgaga gtcgcaacag actcacggcc agctgtccga ggtgccgcac gtcgcgcgat
     6001 gtagccacac cgggacggct gtcctgggcc agcacgacgc gcgcaatttt tttcgcctca
     6061 tgtccggccc gctcgagctc ggccacggat ttcgacatgg cgatggtcgc gcgcagatcc
     6121 cctgccaccg gctggcggcg cgcgagcagc gtgagctgct cttcatcgag actggtgtcg
     6181 taggcgctga cctgggtctc acgctgcaga acgcgctcgg ccgctgcggc gttccattcc
     6241 gtataggcgt aagcggcctc gcgcacctgc tcgagtacca gtccgcccat ctcgacaacg
     6301 cggatgtgca atccggcgag ggcgccatcg aatgccttgc tgatgtggcc ttcgagcatg
     6361 caaccggctt ccagcgttac ccgaagcggc cggttatgta gtcctcggtg agccggtgcc
     6421 gcgggttggt gaataccctg tcagtctcgc cgacctcgat gagtgacccc atatgaaagt
     6481 acgccgtccg ctgcgacacg cgcgctgcct gctgcatgga gtgcgtgacg atcacgatcg
     6541 cgtagctcgc ccgcagctgc tcgatgagat cctccacacg cgcggtcgcg accggatcga
     6601 gggccgagca cggctcgtcc atcaggatga cttccggctg cacggcgatc gcacgcgcga
     6661 tgcacaacct ctgctgctgg ccacccgaga ggccggtgcc gggccgattg agccgatcct
     6721 tcacctcatc ccacaggccc gcgcgctgca ggctggtgca gacgatctca tcgagatcgg
     6781 cacggctgcg ggggattccg tgaatcctcg ggccataggc cacgttctcg tagatcgact
     6841 tgggaaaggg attcggcttc tggaacacca ttccgactcg cgcgcgcagc tggaccacat
     6901 cctggcgctt gtcgtgaatg tcggtcttat cgagatggat cgacccggtg acccgcgcac
     6961 cggggatggt gtcgttcatc cggttgagac agcgcagaaa ggtggatttg ccgcagcccg
     7021 agggcccgat catcgccaac acctgcctgc gagccacatc gatgctgaca cttctgagcg
     7081 cgtgcttgct gccgtagaac acattcacgt cgcgacacgt gaacacggca tccgcgaccg
     7141 atgcctgccc gacagtctgc tcgcccgcgc gctgctcgag agatgccgca gccggagtgc
     7201 ggccactcgg cttgaggttg agacctccct cgccgatccg gcgccggtgc ggctcctctg
     7261 gaatgcccga agcacccatc gccctgcgct cctgtgttac atcattgtgc acatcattca
     7321 tgaaggatca tccgaatcgg cccgtcacgt agtcatccgt ccgccggtca cggggtgcgg
     7381 tgaaaagctg ctccgtcgcc ccgacttcca ccagctcccc catgtacatg aacgccgtaa
     7441 cgttcgaaac gcgcgcggcc tgctgaagat tgtgtgtcac gaccgcgaga cagtatttct
     7501 cgcgcaggct ctgtaatgtt tcctcgaccc gcaacgtgga gatgggatcg agcgcggccg
     7561 ttggctcgtc tagcaacaca acctcgggtt cgagcgcgat ggtacgcgca atgcagagac
     7621 gctgctgctg ccccccggac aggccccggc catcctcgtg cagcttgtcc ttgacctcat
     7681 cccacatcgc tgcatcccgc aacgccacct cgacgcgctt ctcgagctcg ctgcgcggga
     7741 tgcgccgcgc caggcgcagg ccgaaggcaa cgttgttgaa tatcgacatc gggaacggcg
     7801 tggccttctg gaataccatg ccgacccgga agcgcagcgc agacaggtcc accgtcggtg
     7861 cgaggatgtt gagtccgtcg agcagcacct cgccggttgc ataatgccct ggataaaggg
     7921 catacatgcg gttgaagacg cgcagcagcg tcgatttgcc gcagcccgaa ggcccgatga
     7981 acgcggtgat gctgcgatcg gccaggctca gactgatgtc tttcagcgcg cggttctggc
     8041 cgtagtagaa gtccaggtgc cggacctcga atttcggggc gtgggcctcg agggaatggc
     8101 cgttcgcgcg cacgctcgcg gcgctggcgc tcatcgctgc ctcgagccgg tgggattgag
     8161 cgccgagacg acgatgcgtg ccgcgacgct cagcaccagg atggcgaaag tgatcagcag
     8221 cgcaccggac caggcgagct gctgccagtt cttgtagggg ctgagcgcga actggaagat
     8281 gatcaccggc agattggcga tcggcgcgcg caggttggtg ctccagaact gattgttcag
     8341 cgcggtgaac agcagcggcg cggtctcgcc actgatgcgc gcgatggcaa gcagcaggcc
     8401 ggtcacgatg ccactgcgcg ccgcagggta gatgatgctg gtgatggtac gccacggata
     8461 tgcccccatc gcagtggagg catctttcat gccc
//
LOCUS       DASRHN010000124         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2347828, whole genome shotgun sequence.
ACCESSION   DASRHN010000124 DASRHN010000000
VERSION     DASRHN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347828"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VFB37_04755"
     CDS             <1..1421
                     /locus_tag="VFB37_04755"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZO21794.1"
                     /translation="GWFTDDTPKRFEAYGWHVEPEVDGYDPQAIAAAIRSSQAQTSRP
                     SLICCKTIIGFGAPHKQGTKAVHGEALGADEVAAARKELNWPYEPFVIPDELRAQWDQ
                     RRKGASEEAAWRDLFMRYRDAHPQLAREFERRVAGELPRNWRETAAQALAAATRQSSA
                     QATRQSSQAVLDVIGPALPEIFGGSADLTGSNNTLFKGAHSLTREDPAGNYLHWGVRE
                     FGMTAAMSGMALHGGLLPYGGTFLVFSDYARNAVRLECLMHVHVILVYSHDSIGLGED
                     GPTHQPIEHLTSLRAMPHMTLWRPCDAAETAVAWISAVEHRGPTALVLTRQGLPQQQR
                     TPGQLEQIRRGGYVLIDCDGVPEALVIATGSEVGIAAQAVNALKAAGRRVRLVSMPCN
                     EVFDAQDEAYRESVLPKAVRARLAVEAGATLGWWKYVGTEGRVLGIDRYGASGKASDL
                     FPHFGFTADNVESQLRGLLGAR"
     gene            1452..2468
                     /gene="gap"
                     /locus_tag="VFB37_04760"
     CDS             1452..2468
                     /gene="gap"
                     /locus_tag="VFB37_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZO21795.1"
                     /translation="MAIKVGINGYGRIGRNILRALFESKRTSEVQIVALNDLGDAKTN
                     AHLTRYDTAHGPFPGEVAVDGDSMVVNGQRIRVLAERDPAKLPWGSLEVEFVLECTGL
                     FTSKTKASAHLKGGAKKVVISAPGGEDVDATIVYGVNHKVLKREHTVISNASCTTNCL
                     APLAKVLHEKVGITAGIMTTIHSYTNDQVLTDVYHSDLRRARSATMSQIPTKTGAAAA
                     VGLVLPELKGKLDGFAVRVPTINVSLVDLTFTARKATSVEEVNSAMKEAAAAAAWQGI
                     LAYNDAPLVSVDFNHNPHSSIFDATLTKVIDGNLVKACAWYDNEWGFSNRMIDTTVAW
                     SKVS"
     gene            2465..>3646
                     /locus_tag="VFB37_04765"
     CDS             2465..>3646
                     /locus_tag="VFB37_04765"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZO21796.1"
                     /translation="MKVLRLTDLDLREKRVLIREDLNVPVQNGTVSSDARIRASVPTL
                     RRALEQRAKVFILSHLGRPEEGKYDEALSLQPVAARLSELLGKAVPLRRDWLDGVDCQ
                     PGDAVLCENVRFNKGEKKDDEKLARKMAALCDVFVMDAFGTAHRAEASTHGVARFAPV
                     ACAGPLLVGELEALETALHKPARPLVAIVGGSKVSTKLTVLESLLEKVDKLIVGGGIA
                     NTFLAAQGLPVGKSLHEAEMLDVARRLMSKARERGTEIPMPTDVVVAKEFAATAHADV
                     RGINEVRPDEMILDIGPDTAESFGERVRSAGTIIWNGPVGVFEFDQFGEGTRILAQAI
                     ARSKAFSLAGGGDTLAAIEKYAVEDGISYISTGGGAFLEFVEGKTLPAVAVLEERARE
                     SV"
BASE COUNT          654 a         1168 c         1223 g          601 t
ORIGIN      
        1 agggctggtt caccgatgac acgccgaagc gcttcgaggc ctatggctgg catgtggagc
       61 cggaggtgga cggttacgat ccgcaagcca tcgcggcggc gatccgctcg tctcaggcgc
      121 aaacctcgcg cccctcgttg atctgttgca agacgatcat tggcttcggc gccccgcaca
      181 agcagggcac gaaggcggta cacggtgagg cgctcggcgc ggatgaagtg gccgctgccc
      241 gcaaggagct caactggccc tacgagccct tcgtgattcc ggatgagctg cgtgcgcagt
      301 gggaccagcg gcgcaagggc gcctcggaag aggccgcctg gcgcgacctg ttcatgcgct
      361 atcgcgatgc gcatccgcag ctggcccgtg agttcgagcg ccgcgtggcc ggtgagctgc
      421 cgcggaactg gcgcgagacc gcggcccagg cgctggccgc tgccactcgc cagagttccg
      481 cgcaggccac gcgccagtcc tctcaggccg tgctggatgt catcgggccc gcgctgccgg
      541 agatcttcgg cggctcggcc gatctgaccg gctccaacaa caccctgttc aagggcgcgc
      601 actcgttgac ccgcgaggac cccgccggca attatctcca ctggggcgtg cgtgaattcg
      661 gcatgacggc tgcgatgagc ggcatggcct tgcatggcgg actgctgccc tacggcggaa
      721 ccttcctggt gttctccgac tatgcgcgca atgccgtgcg cctcgaatgc ctgatgcacg
      781 tgcacgtcat cctggtctac agccacgatt cgatcgggct gggggaggac gggcccacgc
      841 accaacccat cgaacacctg acgagcctgc gcgcaatgcc ccacatgaca ctgtggcggc
      901 catgcgatgc ggccgagacc gccgtcgcgt ggatctcggc agtggagcac cgcggtccca
      961 cggcgctcgt cttgacccgg caaggcctgc ctcagcagca gcgcacgccg gggcagctcg
     1021 agcagatcag acggggtggc tacgtcctga tcgactgcga cggagtcccc gaggcgctgg
     1081 tcatcgcgac cggctccgag gtaggcattg ccgctcaggc cgtcaatgcg ctgaaagccg
     1141 cgggccggcg cgtgcgcctg gtgtcgatgc catgcaatga ggtctttgac gcccaggatg
     1201 aggcctaccg ggaaagtgtt ctgcccaagg ccgtcagggc gcgcctggcg gtggaggccg
     1261 gggcgacgct cggctggtgg aaatatgtcg gcaccgaggg ccgggtgctg ggcatcgacc
     1321 ggtatggcgc ctcgggcaag gcttcggatc tgtttcccca cttcggcttc acagccgata
     1381 atgtagagag tcagctgcgc ggcctgctcg gggcgcgctg acaaccgaca cacatcagca
     1441 gaggacgagg aatggcaatc aaggtgggca tcaacggcta cggccgcatc gggcgaaata
     1501 tcctgcgcgc gctcttcgag tccaagcgca cgtccgaggt gcagatcgtg gcgctcaacg
     1561 atctgggcga tgccaagacc aacgcgcacc tgacccgcta cgacacggcc catgggccct
     1621 tcccgggtga ggtagcggtg gatggcgatt cgatggtggt caacggccag cgcatccgcg
     1681 tcctcgccga gcgcgacccc gccaagctgc cctggggctc actcgaggtc gagttcgtac
     1741 tcgagtgcac cggccttttc acgagcaaga ccaaagcgtc cgcccatctc aagggcggcg
     1801 ccaagaaggt ggtgatctcc gctccgggcg gtgaggacgt cgatgcgacg atcgtctacg
     1861 gcgtgaatca caaggtgctg aaacgcgagc acaccgtcat ttcgaacgcc tcctgcacga
     1921 ccaactgcct ggctccgctc gccaaggtgc tgcatgagaa ggtcggcatc accgccggga
     1981 tcatgacgac catccattcg tacaccaacg atcaggtgct cacggatgtg tatcactcgg
     2041 atctgcgccg ggcccgctcg gcaaccatgt cgcagattcc gaccaagacc ggggccgccg
     2101 cggccgtcgg gctcgtgctg ccggagctca agggcaagct ggatggcttc gccgtgcgtg
     2161 tgccgaccat caatgtgtca ctcgtggacc tgacattcac agcccggaaa gccacctccg
     2221 tggaggaggt gaacagtgcg atgaaggagg cggccgccgc ggcggcatgg caaggcattc
     2281 tcgcctacaa cgacgcgccg ctggtctcgg tggacttcaa tcacaaccca cactcctcga
     2341 tcttcgatgc gaccctcacc aaggtgatcg acggcaatct ggtgaaggcg tgcgcctggt
     2401 atgacaacga gtggggtttc tccaaccgga tgatcgatac gacggtcgcc tggtcaaagg
     2461 tgtcatgaag gtcctgcgtc tgacggacct ggacctgcgc gagaaacgcg tcctcatccg
     2521 cgaagacctc aacgttccgg tgcagaacgg aaccgtgagc agcgacgccc gcatccgggc
     2581 ctcggtgccg accctgcgtc gggctctgga gcagcgcgcg aaggtcttca tcctctcgca
     2641 tctgggtcgg ccggaggaag gcaagtacga tgaagcctta tcgctgcagc cggtcgccgc
     2701 gaggctctcc gagctgctcg gcaaggcggt tcccctgcgc cgcgactggc tggacggcgt
     2761 cgactgtcag ccgggcgatg ccgtgctgtg cgagaacgtc cgcttcaaca agggagagaa
     2821 gaaggacgac gaaaaactcg cacgcaaaat ggcggccctc tgtgatgtat tcgtgatgga
     2881 cgccttcggc accgctcacc gcgctgaagc gagcacgcac ggtgtcgcac gtttcgcccc
     2941 ggttgcctgc gccggcccgc tgctcgtcgg cgagctcgag gctctggaaa ccgctctgca
     3001 caaaccggcg cggcccctgg tcgcaatcgt gggtggctcc aaggtctcaa ccaagctgac
     3061 ggtgctcgag tcgctgctgg agaaggtgga caagctgatc gtcggcggcg gcatcgccaa
     3121 cacgttcctg gccgcgcagg gtctgcctgt cggcaagtcg cttcacgagg cggagatgct
     3181 cgatgtcgca cggcgcctga tgagcaaggc cagggagcgc ggcacggaga ttcccatgcc
     3241 caccgatgtg gtcgtggcca aggagttcgc cgccacggcg cacgctgacg tgcgcggcat
     3301 caacgaagtg aggcctgatg agatgatcct ggacatcgga ccggacaccg cggaaagttt
     3361 tggcgagcgg gtgcgctcgg cgggcacgat catctggaac ggaccggtgg gcgtgttcga
     3421 attcgatcag ttcggcgagg gcacacgcat cctggcccag gcgatcgccc gcagcaaagc
     3481 cttctccctc gcaggcggcg gcgatacgct cgccgcgatc gagaagtacg cagtcgaaga
     3541 tggcatttcc tatatctcca ccggcggcgg agccttcctg gagttcgtcg aaggcaagac
     3601 gttgcccgcg gtggcggttc tggaggagcg tgcgcgggaa tcagta
//
LOCUS       DASRHN010000125        10924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1194965, whole genome shotgun sequence.
ACCESSION   DASRHN010000125 DASRHN010000000
VERSION     DASRHN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194965"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFB37_04770"
     CDS             complement(<1..239)
                     /locus_tag="VFB37_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZO21797.1"
                     /translation="MIDLTVNGQTHSIDADPQTPLLYVLRDDLQLNAAKFGCGLGQCG
                     ACTVLIDGAAALACVTPILLLSGRQITTLEGLGTL"
     gene            complement(232..1500)
                     /locus_tag="VFB37_04775"
     CDS             complement(232..1500)
                     /locus_tag="VFB37_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZO21798.1"
                     /translation="MKLLPGGRIIMGVIAWVALALGACLLLAWRTAIEPIVRPAVSEF
                     DPAVVHRGAQLAALGNCASCHTAPGGEPLTGGRALSTPFGTLYSTNITPDPKTGIGHW
                     SRAAFRRAMREGVDREGRQLYPAFPYDHFTLVNDTDDDALYAYLMTRRSVPALPRENQ
                     LRFPMNVRWLLGTWKLLFFREGQFQADAAHDAQWNRGAYLVNGLGHCGACHTPRNRFG
                     AEIPAQRLAGGDAQGWYAYAINSASQARVPWDAAALSDYFRTGWQSDHGVAHGPMAEV
                     TNALAEIADSDRSAMSTYVGWMMSQEHRAHGATKLNGPADRDGALVYAGACARCHDGT
                     RALPFGGIRLQFSTAVSAANATNLINVVLDGIGPSEVSAGPIMPGFASTLSDSQLESL
                     LAYVRVHFGSQPPWPAIAESVHQGRRRAHD"
     gene            1620..2084
                     /locus_tag="VFB37_04780"
     CDS             1620..2084
                     /locus_tag="VFB37_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO21799.1"
                     /translation="MPRPPLPPYTAETAAQKVRMAEDAWNQRDPRTVSLAYSEDSRWR
                     NRAEFIRGRAEIAAFLTRKWARELDYRLIKELWAFHRNRIAVRFAYEWHDDSGQHFRS
                     YGNENWEFDDDGLMRVRIASINDLPITAAERKYQWALGPRPQSHPGLSELGL"
     gene            2155..3039
                     /locus_tag="VFB37_04785"
     CDS             2155..3039
                     /locus_tag="VFB37_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZO21800.1"
                     /translation="MLELVIDQRRRDLGGFEVGRVLPFATRRTIGPFIFFDHIGPADF
                     APGLPPGADVRPHPHIGLSTVTYLFEGEIMHRDSVGSEQAIQAGEVNWMTAGRGITHS
                     ERFEKIRTQGGALHGIQSWVALPGHDEETDPAFSHHSRDELPVLEAGGVWMRLIAGRA
                     YGVSSPVRTHSPLFYAHCAMKEGSRVQLPPEYSERAAYIAHGAVEVDRRRYTAGQMLV
                     FSGAHAPASLTALGPAIVMTLGGEPLGERFIEWNFVSSSLERIEQAKADWRAGRMKLP
                     DLDNSEFIPLPALRRPSE"
     gene            3036..3587
                     /locus_tag="VFB37_04790"
     CDS             3036..3587
                     /locus_tag="VFB37_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZO21801.1"
                     /translation="MSVTQPQPDLRVPPQAADHSLGPEHAPVTVVEYGDFECPNCKQA
                     APALRLLLERFAGRVRLIFRHFPLEEVHPHALGAAEAAECAAGQGSFWQMHDLLFANQ
                     DHLEPAQLHGYAEQLRLDMPRYTAGMDEHLYLQRIREHMDGAQRSGVRGTPAIFVNGR
                     IQDVSFGIRALFDAVQSSLQHHR"
     gene            3614..5101
                     /locus_tag="VFB37_04795"
     CDS             3614..5101
                     /locus_tag="VFB37_04795"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZO21802.1"
                     /translation="MDERRSMTAALMVQGCGSDVGKSVLVAGLCRLFTNRGLRVRPFK
                     SQNMSNNAAVTCDGGEIGRAQALQAHACRAEPSTDMNPILLKPQSDLAAQLIVRGRMA
                     GILDASAHGRDKRALLEVAVECYGRLAREAQLVIVEGAGSPAETNLRRHDIANMGLAR
                     ALNLPVVLVGDIDRGHVIASLVGAHAVLDEADRAHIRGFIINKFRGDRTLFEEGLQCI
                     RAHTDWPSLGIIPWLRQAAQLPSEDGVQLEGEGGADIGRPSRPRIVIPLLSRIANFDE
                     FDALRAEPNLDVQFVPPGRPLPPEAALIILPGTKSTIADLAFMRAQGWDIDLLAHVRR
                     GGRVLGICGGYQMLGQALSDPEGIEGPPQSVRGLGLLEVTTVMAPHKTLRVVSGVLLP
                     GRDPFNGYEMHVGTTEGAATCRPLLELSDGTVDGASTADGRIAGSYVHGLFATTAARS
                     ALVRVLGVIPGHADHAATVDSALDGIAGALEECLDIEALAAIASL"
     gene            5277..7061
                     /locus_tag="VFB37_04800"
     CDS             5277..7061
                     /locus_tag="VFB37_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZO21803.1"
                     /translation="MWTVRAARARWPLACLISLSVLAVSPVWLSACRSGRNEADERLR
                     SLYTTEWKWREQQLADGEDAQKPVVDHLPRVDPAAQLTRLRYWEAVLAKLDGIRPAQL
                     SPRERLNYEVYRPQIEALVSNQRFRDYEMPANSDTTFWTNLGYTARRPFRTLQDYTSW
                     IKQLHDVPRYFHEQIGEMRAGLKRGFTPPRVTMQGRDASITAVTDATPEASLLYTPFR
                     EMPGIAPTDQASLRAQAVETIREFVQPAYRELLEFMRTEYVPGCRASLAARDLPDGGA
                     YYRAKIREFTTLDMDAAAIHALGESEVERLHAQMLASMRETGFTGDFPAFLQHLRTDP
                     QFYVGTPDELLMRAAWIAKKFDGKASQFFGYLPRARFAIKPVPADMAPFYTAGRGGPG
                     VYLLNTYNLPARPLYNLTALTLHESAPGHAFQIPIAAEHTDQPEFRRHTYISAYGEGW
                     ALYCELLGVEMGMYETPYDRFGMLGYQIWRAARLVVDTGIHAQGWSREQAIAYLQKYT
                     ALPQHEIETEVDRYIAWPGQALSYYLGERAILQAREKAQHVLGSRFNIRAFHDTVLEL
                     GSVPLPVLTARIDRFITEGGQGPYPGME"
     gene            7073..8467
                     /locus_tag="VFB37_04805"
     CDS             7073..8467
                     /locus_tag="VFB37_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO21804.1"
                     /translation="MQGEGPKTPRPAEPATAQAGEDSLARSLHSRHIQLIAIGGTIGV
                     GLFLGSAGAIHKAGPGLLLNYAIGGIAIFFIMRALGELLTYRPVAGSFASYAEEFVGP
                     FAGFVTGWSYWFMWVVTAMAELTAIGIYVNFWLPDIPQWLPGLCALIVLYATNTLAVR
                     VYGELEFWFALLKVITIVALIVAGLIVIVFGVGSLGATASFANLWKQGGFMPFGLLGV
                     LLTLQIVMFAYSGVELVGVTAGEAQNPAVVLPRATNSIIFRILLFYIGSLVIIMALVP
                     WNQLDPNMSPFVLMFDKMGIPGAANFINVVVITAAASSCNGGVYSTGRMLHSLALRGQ
                     APRAFATLSSRQVPLAGVNASAAMMLIGVVLNYIVPKEVFTWVTSIALIGTLWTWIII
                     MLAHRGYRQAVREGRVRAVSYRMPGWPAANWLVVAFLLIVAAMFALDPDTRVALYVAP
                     LWFALLALGYRFAR"
     gene            complement(8476..10305)
                     /locus_tag="VFB37_04810"
     CDS             complement(8476..10305)
                     /locus_tag="VFB37_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZO21805.1"
                     /translation="MSVLQKTLAIVLLILLGGALYGWWYTNQADSTPVRPGKTLQQAD
                     AASGVNQGTFMLAQRLAALATGPEEQRLAASAERLADHELDLAFTAALRRLEAHPPQL
                     SAEARSVQDRLDKSQKQLESDQASVARLTAAVAQATDSQKGRLQDQLDLASSQLELDK
                     DEIDEANEDLLQAGGNQHQRVQMMMQEHAAAQSRPAATTSSSVAPQATGRMGGLVGEL
                     REWLALHRKQEWLLYAGSQAASNAAALSTEHQRLAVRLQSLKEQTPQLAGHAQADPAA
                     PAAPARPADSASLATTTRRIAADQQRLTLLDQRIAALNQLSGVYGNWNAVSAAKSFGI
                     LHGILSGVAVVVATLLLLLFLDRWLQRLLGRTRLDRRQVETLRSVTRVALQIVGVLII
                     FVIIIGVPGQLGTMIGLAGAGLTVALRDFIVAFIGWLVLMGRNGVRLGDWVEINGVSG
                     EVVELGMFHTVLLETGNWNDTSHPTGRRVTFTNSFAVEKHYFNFSTSGQWLWDELLVL
                     VPFDKDPNLIAEAIQKEVAAATEKSVAEAEAEWGRASASRRGKTLSAAPGVSLKPAVG
                     GVEVSVRYITRASERFALRARLFQAAVRLLGKGIGQPPVTAPG"
     gene            10496..>10924
                     /locus_tag="VFB37_04815"
     CDS             10496..>10924
                     /locus_tag="VFB37_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21806.1"
                     /translation="MRQRWLRAFRRAPRAVQVGALVVILATLSLCINWLYQVVRKPSE
                     LFFPVSGALYKTPAETWRDYEPAFSRYSTPVMTAALLAALAQVEGSGNPVARTYWRWS
                     WSLRPFEVYRPASSAVGMYQITDGTFAEARHFCIHDHTVAE"
BASE COUNT         1872 a         3509 c         3574 g         1969 t
ORIGIN      
        1 ctgagcgtgc ccaggccttc cagtgtcgtg atctgccgtc cggaaagaag cagaatcggt
       61 gtcacgcagg cgagggcggc agccccatcg atcagcaccg tacaagctcc gcactggccg
      121 aggccacagc cgaacttggc tgcgttgagc tgcaggtcat cccgcaggac gtagagcagc
      181 ggagtctgcg gatccgcatc gatggagtga gtctgtccat tgacggtcag gtcaatcatg
      241 cgcgcgcctc cggccctggt gtacggactc ggcgattgcc ggccacggcg gctgactgcc
      301 gaagtgcacg cgcacgtacg cgagcaatga ctcgagctgg gagtccgaga gcgtgctggc
      361 gaagccgggc atgatgggtc ccgcgctgac ctcggagggg ccaataccat cgaggaccac
      421 attgatgagg ttggttgcat tcgcagcgct cactgcggtg ctgaactgca ggcggatccc
      481 tccgaaaggc agtgctcgcg tgccgtcgtg gcatcgcgca caggcgcctg cgtaaacgag
      541 cgcgccatcc ctgtcggccg ggccgttgag cttggtcgcg ccgtgcgcgc ggtgctcctg
      601 gctcatcatc catccgacat aggtcgacat cgcactgcgg tccgaatccg cgatctcggc
      661 cagagcgttg gtcacctccg ccattggccc gtgcgccacg ccatgatcgc tctgccagcc
      721 ggtgcggaaa tagtcgctga gcgcagcggc atcccagggc acgcgcgctt gcgaagccga
      781 attgatcgcg taggcgtacc agccttgtgc atcgcccccc gccaggcgct gtgctggaat
      841 ctcggcccca aaccgattgc gcggcgtatg gcaggctcca cagtgcccga ggccgttgac
      901 gagataggca ccgcgattcc actgcgcatc gtgggcggcg tcagcttgaa actgcccctc
      961 gcggaagaac agcagcttcc aggtcccgag caaccagcga acgttcatcg ggaacctgag
     1021 ttgattctct ctcggcagtg cgggcaccga acgccgcgtc atgagatacg cgtacagcgc
     1081 atcatcgtcc gtgtcgttga ccagtgtgaa atgatcgtat gggaacgccg ggtacagctg
     1141 tcggccctca cggtccacgc cctcccgcat ggcccgacgg aatgccgccc tcgaccaatg
     1201 tcctattccc gtcttcgggt caggtgtgat gttggtggaa tagagcgtac cgaagggcgt
     1261 ggagagggct cgtccacctg tcagcggctc tccaccagga gcggtatgac aggatgcgca
     1321 attcccgagg gcagcgagct gcgcgccgcg atgaacgacg gctggatcaa actcgctcac
     1381 ggcaggtcgc acaatgggtt cgatagcagt gcgccacgcc agaagcagac aggctccgag
     1441 cgccagtgcc acccatgcga tgacccccat gatgatgcgc ccgcccggga ggagcttcat
     1501 tgtggaaatg caccttgccg aactgtagcg ctagcttgcc tgcgagaacg ggccgtccgc
     1561 agcaagtgta tattgcgggg gcatctgatc gctcgaaccg cttcctcacg gagggccgca
     1621 tgccacgccc gcccctgcct ccctacaccg ccgagacggc cgcgcaaaaa gtccggatgg
     1681 ccgaggacgc ctggaatcag cgcgatccga ggaccgtatc gctggcttac agcgaggaca
     1741 gtcgctggcg caaccgggcc gaattcatcc gtggccgcgc cgagatcgct gccttcctca
     1801 ctcgaaagtg ggctcgcgag ctcgactacc gcctcatcaa ggagctctgg gcattccacc
     1861 gcaaccgcat cgccgtgcgc ttcgcctatg agtggcatga tgacagcggt cagcactttc
     1921 gctcctacgg caacgagaac tgggagttcg acgacgacgg actgatgcgc gtgcgcatcg
     1981 ccagtatcaa tgatctgccg atcacggccg cggagagaaa gtatcagtgg gccctgggac
     2041 cgcggccgca gtcccatccg ggtctgagtg agctgggcct gtagcgcacg cacgctggtt
     2101 tgccagggtc ggcgaaaagc tcgatgatgt gactcccact gcgtccttcc catcatgctc
     2161 gagctcgtga tcgatcagcg ccgtcgcgat ctgggcggct tcgaagtggg acgggtcctg
     2221 ccgttcgcaa cccgccggac catcggtccg ttcatcttct tcgaccacat cggcccggcc
     2281 gattttgctc ccgggctgcc ccccggtgcc gatgttcggc cccatcccca tatcggcctg
     2341 tcgacggtca cgtacctgtt cgagggagag atcatgcacc gcgacagcgt ggggtccgag
     2401 caggccatcc aggcgggcga ggtgaactgg atgacagcag ggcgcggcat cactcactcg
     2461 gaacgtttcg aaaaaatccg tacgcaaggc ggcgcgctgc acggtattca atcctgggtc
     2521 gcacttccgg gtcatgatga ggaaaccgac ccggctttct cccatcactc tcgcgatgag
     2581 ctgccggtgc tggaggctgg aggggtgtgg atgcgattga ttgccgggcg cgcgtacggg
     2641 gtcagctcgc ccgtccggac tcactcgccg ctcttctatg ctcactgcgc gatgaaggaa
     2701 ggctcacgcg tacagcttcc gcccgaatac agcgagcgtg ccgcgtatat cgcccacggt
     2761 gcggttgaag tggaccgccg ccgctacacc gccggacaaa tgctcgtgtt ctccggagcc
     2821 cacgcgcccg cgagcctcac tgccctgggt cccgcaatcg tcatgacgct cggaggcgag
     2881 ccgctcgggg agcgcttcat cgaatggaac ttcgtttcct cctccctcga gcgcatcgag
     2941 caggcgaaag ccgattggcg cgccgggcgg atgaaactcc ccgatctgga caattccgag
     3001 ttcattcctc tgccagcact caggaggcca tccgagtgag tgtcacgcag ccgcagcccg
     3061 acctgcgcgt gcccccgcag gccgccgatc actccttggg tcccgaacat gcgccggtga
     3121 cagtcgtcga atacggcgat ttcgaatgtc ccaactgcaa gcaggcggct cccgctctca
     3181 gactgctgct cgagcggttc gccggccgcg tacggctcat cttccgacat tttcccctcg
     3241 aggaggtcca cccgcacgcg ctcggagcgg cggaggcggc cgagtgtgct gccggccagg
     3301 gcagcttctg gcagatgcac gatcttctgt ttgccaatca ggaccatctc gagccggccc
     3361 agctgcacgg atacgccgag caattgcggc tggacatgcc gcgctacacg gccgggatgg
     3421 acgagcattt gtatctgcag agaattcgcg agcacatgga tggcgcgcaa cgcagtggag
     3481 tacgtggcac ccctgcgata ttcgtcaacg gtcggattca ggatgtctcg ttcggcatcc
     3541 gcgcgctgtt cgatgcggtg caatcgagcc tgcaacacca ccgctgatgg acacaggtgc
     3601 ctgacatcct ccggtggacg aacggcgctc gatgaccgcg gcgctgatgg tgcagggttg
     3661 tggctcggac gtcggcaagt ccgtgctggt ggccgggttg tgccgcctgt tcaccaatcg
     3721 cgggctcagg gtccggccct tcaagtcgca gaacatgtcg aacaacgctg cggtcacgtg
     3781 cgacggcggc gagatcggcc gggctcaggc tctgcaggcg catgcctgtc gcgcagagcc
     3841 ttccacggac atgaatccca tcctgctgaa gccacagagc gatctggcag cgcagctcat
     3901 cgtgcgcggt cgcatggcag gaatactcga tgcgagcgcg cacggccgtg acaagcgggc
     3961 gctcctcgag gtcgcggtgg agtgctacgg gagactcgcc cgcgaagcgc agctcgtgat
     4021 cgtggagggg gcgggcagcc cggccgagac gaacctccgc aggcacgaca ttgccaacat
     4081 gggcctggcg cgggccctga atctgccggt cgtcctcgtt ggcgatatcg atcgtggcca
     4141 cgtcattgcc tcgctggtgg gcgcgcatgc cgtgctggat gaggcggatc gcgcgcacat
     4201 ccgtggcttc atcatcaaca agtttcgcgg agaccggacg ctcttcgagg agggactgca
     4261 gtgcatccgg gcgcacacgg attggccgtc cctgggcatc attccctggc tcaggcaggc
     4321 cgcacagctg ccatccgaag acggcgtgca actcgaaggt gagggcggcg ccgacatcgg
     4381 gcggccctcg cgcccccgga tcgtcattcc tttattgtcg cgcattgcca acttcgatga
     4441 gttcgatgcg ctgcgcgctg aacccaatct ggatgtccag ttcgtccccc cgggccgacc
     4501 gctgccgccc gaggcggcgt tgatcatcct tccgggcacc aaatcgacga tagcggacct
     4561 tgcgttcatg cgcgcgcagg gctgggacat cgaccttctc gcacacgtgc gccgtggcgg
     4621 gcgcgtgctc ggcatctgcg gcggctacca gatgctcggg caagctctga gcgatccgga
     4681 agggatcgag ggcccaccgc agagtgtgcg cggtctgggc ttgctggaag tcacgaccgt
     4741 catggctcct cataagacgc tgcgggtcgt ctcgggggtg ctgttgcccg ggcgcgatcc
     4801 gttcaatggc tatgagatgc acgtgggtac gaccgagggt gccgcgacat gccgtcccct
     4861 gctcgagctt tccgacggga cggtcgatgg cgcaagcacg gccgacgggc ggatcgctgg
     4921 cagctatgtt cacgggcttt tcgcaacgac cgcggcccgg tcggcgctcg tccgagtgct
     4981 cggcgtaatc cccggtcatg ccgatcatgc ggccacggtg gatagcgcgc tcgatgggat
     5041 tgcaggcgcg ctggaagagt gtctggatat cgaagcgcta gcggcgatcg cgagcttgtg
     5101 agaatggaac ccacagttcc catgagagca aacggcgtgg ccgaccgtga cattggtggg
     5161 ggcagcagaa ctcgcagcgt tatacttctg gccgctgcaa cgatggaaag gctgacgatg
     5221 cggacaaggg atgcgacggt tgtgtgcgcg ggctcgctct gcgtcgcggt gctttgatgt
     5281 ggaccgtgcg ggccgcgcgg gcgcgttggc cgctcgcctg cctcatcagc ctgagcgtgc
     5341 tcgcggtcag ccccgtctgg ctcagcgcct gcagatcggg ccggaacgag gcggatgagc
     5401 gtctaagatc gctctacacg accgagtgga agtggcgcga gcagcagctg gctgacggcg
     5461 aggatgcgca gaagccggtc gtcgatcatc tgcccagggt cgatccggcc gcacagctca
     5521 cgaggctgcg ttactgggaa gcggttctgg cgaagctcga cggcatccgg ccggcgcagc
     5581 tttccccgcg agagcgactg aactacgaag tctaccggcc gcagatcgag gccctggttt
     5641 ccaaccagcg tttccgcgac tacgagatgc cggccaattc ggacaccacg ttctggacca
     5701 acctgggtta caccgcgcgc aggccgttca ggaccctgca ggactacacc agctggatca
     5761 agcagctgca cgatgtgccg cggtattttc atgagcagat cggagagatg cgcgcgggac
     5821 tgaagcgtgg cttcacgccc ccgcgggtca ccatgcaggg gcgggacgcc tccatcacgg
     5881 cggtcactga tgccactccg gaggcgagcc tcctctacac ccccttcagg gagatgccgg
     5941 gaatcgcgcc aaccgatcag gccagtctgc gcgcgcaggc agtcgagacc atacgagagt
     6001 tcgtgcagcc ggcctacaga gagctgctcg agttcatgcg cacggagtac gtgcccggct
     6061 gccgtgccag cctcgccgcg cgagatctgc cggatggcgg ggcgtactac cgcgcaaaga
     6121 tccgcgagtt cacgacgctc gacatggatg cagcggccat ccacgcgctg ggagagagcg
     6181 aggtggagcg gctgcatgcg cagatgctgg catcgatgcg cgaaacgggc ttcacgggcg
     6241 acttccctgc cttcctgcaa cacttgcgca cggatccgca gttttatgtg ggcacccccg
     6301 atgagctact gatgcgcgcg gcctggatag ccaagaaatt cgacggcaag gcctcacaat
     6361 tcttcggcta cctgccgcgc gcgcgcttcg ccatcaagcc ggtgccggcg gatatggcgc
     6421 cgttttacac cgccgggcgc ggcggaccgg gagtctacct gctcaacacc tacaacttgc
     6481 cggcgcgacc actctacaac ctcaccgctc tgacgctgca cgagtcagcc ccggggcacg
     6541 cctttcagat tccgatcgcg gccgagcaca ccgatcagcc ggagtttcgc cgccatacct
     6601 acatctcggc atacggtgag ggatgggcac tctattgcga gctgctgggt gtggagatgg
     6661 gcatgtacga aaccccatac gatcgcttcg gcatgctcgg atatcagatc tggcgcgccg
     6721 cgcgcctggt ggtggacacc gggatacacg cccagggctg gtcgcgtgag caggcgatcg
     6781 cgtatctgca gaagtacacc gctctgccgc agcatgaaat cgagaccgaa gtcgatcgct
     6841 acatcgcctg gcccgggcag gcgctgtctt attacctggg cgagcgcgct atcctgcagg
     6901 ctcgggagaa agcccagcat gtgctcggat cgcgcttcaa tatccgtgca ttccacgaca
     6961 ccgtcctgga gctgggttcg gtaccgctgc cggttctgac ggcccgcatc gaccggttca
     7021 tcaccgaagg tggtcaagga ccgtatccgg ggatggagta gagagggcga gcatgcaagg
     7081 ggaaggtcca aaaacaccgc ggcccgcaga gccggcgact gcacaggcgg gggaagattc
     7141 cctggcgcgc agcctgcaca gtcgccacat ccagctcatc gcaatcggcg gcacgatcgg
     7201 tgtcggcctg tttctcggct cggccggtgc catccacaag gcgggccccg ggctgctgct
     7261 caattatgcg atcggcggaa tcgccatctt cttcatcatg cgggccctgg gtgagctgct
     7321 cacttaccgg cccgtggccg gctccttcgc gagctatgcg gaggagttcg tcgggccatt
     7381 cgccggcttc gtcacgggct ggtcctactg gttcatgtgg gtcgtcaccg cgatggcgga
     7441 gctgaccgcc atcggcatct acgtgaattt ctggctgccg gatattccac agtggctgcc
     7501 cggcctgtgc gcgctgatcg tgctttatgc gaccaacact ctggcggtac gcgtctacgg
     7561 cgagctggag ttctggtttg cgctcctcaa ggtgatcacg attgtcgccc tgatcgtggc
     7621 gggcctgatc gtgatcgtct tcggggtggg ctcgctcgga gcgacggcga gcttcgcgaa
     7681 tctgtggaag cagggagggt tcatgccctt tggcctgctg ggagtgctgc tcacactgca
     7741 gatcgtgatg tttgcatact ccggcgttga gctcgtcggt gtgaccgccg gcgaggcgca
     7801 gaacccggcc gtggttctgc cgcgcgccac caacagtatc atcttccgca tcctgctctt
     7861 ctacattggc tccctcgtga tcatcatggc tctcgtgccc tggaatcagc tcgacccgaa
     7921 catgagtccg ttcgtgctga tgttcgacaa gatgggaatt cccggggcgg ccaatttcat
     7981 caatgtagta gtcatcaccg cggcagcctc ttcgtgtaat ggcggggtct acagcaccgg
     8041 acgcatgctg cactcgctgg cgctgcgcgg ccaggcgccg cgggcgtttg ccactttgag
     8101 ctctcggcag gtgccgcttg caggggtgaa tgcttcggct gccatgatgc tgatcggcgt
     8161 ggtcctcaac tacatcgtgc ccaaggaagt gttcacgtgg gtcacgagca ttgccctgat
     8221 cggcacgctg tggacctgga tcatcatcat gctcgcacac cggggctatc ggcaggccgt
     8281 gcgcgaaggg cgggtacgcg ccgtgtcgta ccggatgccc ggctggcccg ccgcgaactg
     8341 gctggtggtc gcttttttgc tgatcgtcgc agcgatgttc gcgctcgatc ccgacacgcg
     8401 cgtggcgctg tacgtcgcgc ctttgtggtt cgccctgctc gcgctgggat accggttcgc
     8461 gcgatgacct gagagtcagc cgggggctgt caccggtggc tggccgatgc ccttgccgag
     8521 caggcgaacg gccgcctgga acagccgcgc gcgcaaggcg aagcgctcgc tcgcgcgagt
     8581 gatgtagcgg accgatactt ccactccgcc aaccgctggc ttgaggctga caccgggcgc
     8641 cgcggacagc gtcttgccgc ggcgcgaggc actggcgcgt ccccactccg cctcggcttc
     8701 tgcgacgctt ttctcggttg cggcggcgac ctccttctga atcgcctccg cgatcaggtt
     8761 cggatccttg tcaaacggca ccagcacgag cagctcgtcc cagagccact ggccggaagt
     8821 ggagaagttg aaataatgct tctcgaccgc gaagctgttc gtgaaagtca cgcgccggcc
     8881 cgtaggatgg ctggtgtcgt tccagttgcc tgtctcgagc aggacggtgt gaaacatgcc
     8941 gagctcgacc acctcgccgc tcacgccgtt gatctcgacc cagtccccga gacgcacgcc
     9001 attgcgcccc atcaacacca gccagccgat gaaggcgacg atgaaatccc tgagtgccac
     9061 cgtgagtccg gcccccgcca ggccgatcat cgtgccaagc tgacccggca ctccgatgat
     9121 gatgacgaag atgatgagca cgcccacgat ctgtagcgcg acccgcgtga cgctgcgcaa
     9181 cgtctccacc tggcggcggt cgagccgggt gcgaccgagc aggcgttgca gccatcgatc
     9241 cagaaaaagc aacagcagca gagtcgccac caccacggca accccggaca gaatgccatg
     9301 caggatgccg aaggacttcg ctgcactgac cgcattccag ttgccgtaaa ccccggagag
     9361 ctggttgagg gcggcaatgc gctgatcgag cagcgtcagt cgctgctggt cggccgcgat
     9421 gcggcgcgtc gtagtggcga gtgatgcgga gtcggcaggc cgcgccggcg cggcgggcgc
     9481 ggcaggatcg gcctgtgcgt ggcctgccag ttgaggagtc tgttccttga gactctgcag
     9541 ccggacagca agcctctgat gctccgttga cagagcggca gcgttgctcg cggcctgtga
     9601 accggcgtac agcagccact cctgcttgcg atgaagagcc agccactcac ggagctcgcc
     9661 gacgaggccg cccatgcggc cggttgcctg cggagcgacg gacgagctcg ttgtcgcggc
     9721 ggggcgcgac tgcgcggcag cgtgctcctg catcatcatc tggacgcgct gatgttgatt
     9781 accgccagcc tgcagaagat cctcgttcgc ctcgtcgatc tcgtccttgt ccagttcgag
     9841 ctgcgaggag gcgagatcga gctggtcctg caggcggcct ttctgagaat ctgtggcctg
     9901 agcaacagcc gcggtcagcc gcgccacgct cgcctggtcg ctctcgagct gcttctgtga
     9961 tttgtcgagc cgatcctgga cgctgcgcgc ttccgcgctg agctgaggcg gatgggcttc
    10021 gagccggcgc aacgcggcgg tgaatgccaa atcgagctca tgatccgcga gccgctcggc
    10081 cgaggccgcc aggcgttgct cttccggacc tgtcgccagg gccgccaggc gttgggccag
    10141 catgaaggtg ccctggttga cgcccgaggc cgcatccgcc tgctgcagcg tctttcccgg
    10201 ccgcacgggc gtgctgtctg cctgattcgt ataccaccag ccataaagcg caccgccgag
    10261 caggatcagc aggacgatcg ccagcgtttt ctgcaagacg ctcatgggac tccccggccg
    10321 gatcggcacc gaccgcatgc tagattaagc ggcaggcagc cgcacgtggc gcatgtgccg
    10381 gatcgaagga caggacctga tgccacgccg aatcgctgcc ggatctcccg aacgccatcg
    10441 tgcacgggga cgcggacgcc gcgggcgccg atggtggccc tgctcgaacg cggccgtgcg
    10501 tcagcgatgg ctgcgggcct tcaggcgcgc gccacgagcc gttcaggtcg gggccctggt
    10561 ggtgatcctg gcaacgctct ctctgtgcat caactggctc taccaggtgg tccgcaagcc
    10621 ttcggagctg ttctttccgg tgagtggcgc gctgtacaag acacccgccg agacctggcg
    10681 cgactacgag ccggcattca gcagatattc gacgcccgtg atgacggcgg cgttgctcgc
    10741 ggccctggct caggtagagg ggtcgggtaa tccggtcgca cggacgtact ggcgctggtc
    10801 ctggagcctg cggccgtttg aggtctatcg gccggcatcg agtgccgtcg ggatgtacca
    10861 gatcacggac ggcacgtttg ccgaggcgag gcatttttgc atccacgatc acacggtggc
    10921 agag
//
LOCUS       DASRHN010000126        18157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_39901, whole genome shotgun sequence.
ACCESSION   DASRHN010000126 DASRHN010000000
VERSION     DASRHN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18157
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39901"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFB37_04820"
     CDS             <1..967
                     /locus_tag="VFB37_04820"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZO21807.1"
                     /translation="LDMKRAEDCELFATLIGQADVYIQNFRPGTATRLGAGPERLLEL
                     NPQLVYCSISGFGADGPYAERPSYDSVAQALSGFLSVTVDYRRPQFLGPALADAITGL
                     YASQGILAALVQRGRRGRGCHLEISMLEAMAHFAIEPFAAFFALGETPTSADRPRLAQ
                     AYIVHTADERLIAIHLSSLEKFWRGLVAALEAPELGTDPRFNPRERRIAEYETLRAEL
                     DSRFAQRPLAHWTARLHAEDVPHAPVNRINDVVRDAQVEHLGIVVPVRTPHSATHSVR
                     APWQFAGQRAHAVSAAPLLDEHGAAIRGALAGSKAWPEATQAANS"
     gene            983..2035
                     /locus_tag="VFB37_04825"
     CDS             983..2035
                     /locus_tag="VFB37_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZO21808.1"
                     /translation="MLLPTMLVGSYPQPEWLIDRERLAGRFPPRVRARELWRVPELYL
                     EQAQDDATIVAIKAQEDAGLDFITDGEIRRESYSNRFATALEGIDLDNPGIALDRSGH
                     PNPVPRVVGAIRRRHAVGVRDLQFLRSHTRRQIKVTVPGPFTMAQQAQIDFYGGSREQ
                     AALDYATAVNEEIRDLFEAGADIVQIDEPYMQARPEDARAYGLRALNRALDGVSGITA
                     VHICFGYAAIIHTRPSGYSFLPELAECHCNQVSVETAQSNLDCAVLAQLRGKKIILGV
                     IDLSTATVESAELIATRIRRALPYVDAHELLLAPDCGMKYLPRAAAFGKLQAMVAGAR
                     IVRAQLENENAARAQS"
     gene            2062..4353
                     /locus_tag="VFB37_04830"
     CDS             2062..4353
                     /locus_tag="VFB37_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO21809.1"
                     /translation="MSTATRRNLSVLAGVLSVISFSPASWAQSQQSASPDAVALEEIV
                     VTARKRDESYQDVPITVSVFTEQEIQAAGIESPRDFIAMVPNMTLVETQNVGNSFVTV
                     RGISQARNSEPSVAVLVDGVLETNPYEFSQELFDIRQIEVLKGPQGALYGRDAIGGAI
                     IIRTADPPDHFEATTRVGIGNGVSEKAQLALGGPLDSDGTLRYRAAFNFYNTAGYINN
                     ANLDRDADPYRDYAGRIRLVWKPGDAFSADLRLAFDRTQTTAYYFVIPRSDEANPFTD
                     FSTPGDANNTTSPIQNNNLGTDDRNVFDTALKLDYSPGYGTVTSITDYDETREIDTGD
                     AYDFRPITSSIAFNCPSCIGANPTAAQGGPFDESQSQFIWVRTWSQELRFTSPVVNGF
                     SWIAGGYFVHTDRFISTGNLVDRGFGVPAVYYTPIVDPSNPYATNSNVTFLADSQANN
                     AWALFGDATYDFSKAWELDLALRYDEDRRENTTLTPTPFLPTTAAFYGEKRYATFSAS
                     QPKATLRFRPMEDWTFYGGWSRGFRSGGFNQTGVGEVARDNSVLGVNDLFQAEIADTW
                     EIGTKAELMDRRLSLDADVFYTRSKNGYFFVFIAADSTQNLGNLDATYKGAELSLTAR
                     PTDRLDVYASFGYTASRITAMADPTVIGNEAPLVSRNTVNAGVQYRQPLANGLNGTVR
                     LDYQEIGRTWWEPYNITSRDPVNLLDLRLGIQGEKWDLTAWSKNLTNTIYNAEFSPGN
                     VGGSGFLWRALPRRYGVDFTYKF"
     gene            4372..4908
                     /locus_tag="VFB37_04835"
     CDS             4372..4908
                     /locus_tag="VFB37_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO21810.1"
                     /translation="MAQTKQLPSRLHHTAYVTRDQEKTRQFYEEVIGLPLLATWCESD
                     ELFGKVRTYVHTFYGIGDGGALAFFQFADSADQEEFGPKMPATPFHHIALRVDAETQA
                     AIEGRIAAAGIKPPDTYVLEHGYCRSVYVTDPNGLILEFTRDHQDVEKIGRDRRADAH
                     QALKRWLAGDHTSNNTYR"
     gene            4936..5799
                     /locus_tag="VFB37_04840"
     CDS             4936..5799
                     /locus_tag="VFB37_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HZO21811.1"
                     /translation="MREFDEQSITQAVISRLSDADDPRFKQVMLSLIRHLHDFVREVR
                     LTEAEWFSAIQFLTDTGKTCTDKRQEFILLSDTLGLSALVITLNHPVEDGSVESTVQG
                     PFYWEGAPELPRGSNLAEGVKGEPAFYSGRVLSTDGTPLANALLDIWSGDGEGKYDMQ
                     MAGEAYMQARGRIRTDAEGRYWFRSIRPTFYPVPTDGPVGNMLRRMGRHPYRPGHIHM
                     IVSAPGHHAVTTHLFVAGSPYLDSDAVFGMKESLVAQFERHPPGAGPGGEPLETPFYT
                     VSYDFRLRPTV"
     gene            5816..6961
                     /locus_tag="VFB37_04845"
     CDS             5816..6961
                     /locus_tag="VFB37_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HZO21812.1"
                     /translation="MSAGAGRILTTHVGSLPRSQAVTDVLFAREREELRATDRDEQVI
                     TEAVAEVVRRQVEVGIDLVSDGEMSKISYATYIARRLNGFAGDTPREPGQDLVEFPGL
                     LRKLAERGSTAKYRRPRCVAAVTVKDTRPLATDVANLSAAVASAQPVGMFMNAASPGV
                     VALFQPNDYYRTQDEYLEALAAALQSEYEAIVQAGIILQIDAPDLAMGRHTMYRDRSM
                     EEFESLAARHIEVLNHALRNVPADRVRMHACWGNYEGPHHHDVPMARLLPILLKAKPQ
                     GLLFEAANPRHAHEWAVFQGADIPEEKVLIPGVLTTTTNYVEHPELVAQRLERFAQIV
                     GRDRVIGGTDCGFGTFAGFGPVEPDIAYLKLRSLVEGAAIASERLWR"
     gene            complement(7024..7097)
                     /locus_tag="VFB37_04850"
     tRNA            complement(7024..7097)
                     /locus_tag="VFB37_04850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7061..7063),aa:Arg,seq:tcg)
     gene            complement(7147..8289)
                     /locus_tag="VFB37_04855"
     CDS             complement(7147..8289)
                     /locus_tag="VFB37_04855"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZO21813.1"
                     /translation="MSRRELLAASALLLVPPGAALARAQQARTAVAATTTTAAEAGAA
                     PIILCWNENPYGPSPAARAAITAAVSQGCRYPDDEIGKLVDLLAKKEGVAQDRIVTGT
                     GSGELLRALGMLCGHGGGEIVAAQPTYQELTEYAQGSGAELKWVPVDGKLRHDLTAMH
                     AAVSDRTRAVYICNPNNPTGTVVPASAIEAFVSSMPEHVTTIVDEAYLDFTDAPEVRS
                     VASLTSGSKRVVVLRTFSKIHGMAGVRCGYAIARPDVAKEIAKARMTTPNVFAVRGAC
                     ASLGDHAFLADTRRRILASRKRITSGLSSMGFNYAEPQGNFVFFDTGAPLPRITEFMR
                     KRNILVGRRFPPLDNWCRITIGTEPEVDAFLEGLRAFRASGQSKAA"
     gene            8420..9034
                     /locus_tag="VFB37_04860"
     CDS             8420..9034
                     /locus_tag="VFB37_04860"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZO21814.1"
                     /translation="MPERRRCAWASGLDADYTRYHDEEWGRPVHDDRRLFEMLILEGA
                     QAGLSWATILRKRAHYRRAFADFDPARVARFDARRRAALLKDAGIVRNRLKIESTVSN
                     ARAFLEVQREFGSFDRYLWGFVANRPRIRLATGRRPPARTELSDRISKDLKKRGFRFV
                     GTTILYAFLQAVGVVNDHTRECFLRPRPRRRSPAAARAVRGGRE"
     gene            9092..9481
                     /locus_tag="VFB37_04865"
     CDS             9092..9481
                     /locus_tag="VFB37_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21815.1"
                     /translation="MRVLAAMATVAALAVGPVYADCSYPPPPDKLPDGNTATKDEMLA
                     GQQTVKTYDKAINAYLACIKLEHDGAASKIGDKPSTPEQKKALDDLNRVQIQKNNAAV
                     DQLQSVADRFNEQVRAYKAKHKDDPKG"
     gene            9487..10686
                     /locus_tag="VFB37_04870"
     CDS             9487..10686
                     /locus_tag="VFB37_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HZO21816.1"
                     /translation="MLSPAQADELIGQHVTCLPIESLPLAQCAGAVLRENIYAERDQP
                     PFDRVAMDGIALDSNTVQHGTRRLQIQATQAAGDPPLALATPASCIEVMTGAVVPSGC
                     DSVVPVEQITVDRSEAELTAGVTVEPWQNVHRRGTDTRQGALLLSTGLRLQAPEIAIA
                     ASAGMARIRVSSQPMVAVISTGNELIEPGEPILPHQVRRSNAYAIVAALRRHGFQRVA
                     DDHLLDDPAELKRRLKFHLETHDVLVMSGGVSMGKLDFVPRVLDELGVQTVFHKIMQR
                     PGLPIWFGAASSGATVFALPGNPVSTLMCLTRYALPALFTAMGQLPPAPERMALGGEI
                     KVKSPHTRFMPVKIEVDDWGRAWAMPRPTNGSGDFTSLGGTDGFVELPPGPNNYPKGF
                     VTRLHRW"
     gene            10801..11856
                     /gene="moaA"
                     /locus_tag="VFB37_04875"
     CDS             10801..11856
                     /gene="moaA"
                     /locus_tag="VFB37_04875"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZO21817.1"
                     /translation="MSRPEVVVPLNNLAGVRDTLGRPVRDLRISVMDRCNFRCPYCMP
                     REKYHERYKFLGSHERLSFDEIVRLARLFVKLGVRKLRLTGGEPLLRTNLSDLIGDLT
                     AIPGVEDVALTTNGVLLARQAAELKAAGLRRITVSLDSLDPELFARMSGGFGGLEDVL
                     DGIAHARSAGLEPIKINCVIQRGINDATAVDLVEHFRGTGVIVRFIEYMDVGSRNEWR
                     QDLVVPSKELVARISERWPLQAVEREYRGEVAQRYQFADGAGEVGFISSVTQPFCGDC
                     SRARLSSDGVIYTCLFATHGTNLRDALRSGASDEQLLQIIRSIWLARTDRYSEQRAQL
                     RKTAGDQRKVEMFYIGG"
     gene            11858..12355
                     /gene="moaC"
                     /locus_tag="VFB37_04880"
     CDS             11858..12355
                     /gene="moaC"
                     /locus_tag="VFB37_04880"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZO21818.1"
                     /translation="MPRSSPGDLTHLDARNRPTMVDVGAKDATRRIATAEARIRIPPA
                     VAQALRKSGHRTKKGPVFDTAIVAGVMAAKRTHELIPFCHPLGLDNCAIEIEERRSGA
                     ELVIRCQASVHHRTGVEMEALTGATVAALTVYDMCKALSHEIEITSIRLLEKSGGKRD
                     FRRKA"
     gene            12366..13274
                     /locus_tag="VFB37_04885"
     CDS             12366..13274
                     /locus_tag="VFB37_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZO21819.1"
                     /translation="MSDLSSPAPTLYGLVLAGGRSSRMQRDKAELSFHGRSQLEWTVA
                     LLEPHVQRVFVSVRPDQVDDPIRARFAQVVDERAGLGPIAGILAAQARYPRAAWLVLA
                     CDLPYVDQTTITHLIRAREPHKQATAYRSSYNGLPEPLCAIFEPSTREALLAYAGRGK
                     DCPRKFLINSDIQLLDQPNPRALENVNTPAERDSAMTALSSKEASPGRPRASGRPEVA
                     LSMTSKRIKVQYYALLREQAGRSDETLLTSARTPRELYEELKARYPFSLAPEMLRVAV
                     NAEFGEWGNPLADGDFVVFIPPVAGG"
     gene            13277..14890
                     /locus_tag="VFB37_04890"
     CDS             13277..14890
                     /locus_tag="VFB37_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZO21820.1"
                     /translation="MQRVASFRFSHEKIDEASLREQLADPAAGAYTSFEGWVRNHNEG
                     LSVHHLEYEAFEPLAVKEGERIVAEAVARFGVEHAACVHRTGDLAIGEMAVWVGVSAP
                     HRHEAFLACRYIIDEVKHRLPIWKKEHYENGDSGWVNCERCAEHGGAEHDSSQHHHPN
                     TSRADHVLVPDYSRQMALREVGAAGQAKLRAGKVLVVGCGGLAVPVVGYLAGAGIGRL
                     GLLDSDRLEPSNLHRQTMYALSDVGQLKAELAAERARALNPEVEARAHVLRLDAQNAG
                     ELIAQYDLVVDCTDNFATKFLLNDFCVLLNKPVVFASVYQYEGQLQVVRPLRDAESEN
                     RSGACLRCVWPEATRDGIVGNCAEAGVLGPVPGVFGALQAFEALKLLLDLPGQLTDEL
                     LVLDLLTLSIARVRTRRAAACPQHAISRAESAQKAASHAASDTVPELEVSFDSLEQAL
                     DAGFELVDIREAEEVSETPAPSRQVKHVPMAQLLYGKTELEPRAKSLLICASGRRSLA
                     ATQELRARGLENVYSLRGGVTALQGRLST"
     gene            15151..15795
                     /locus_tag="VFB37_04895"
     CDS             15151..15795
                     /locus_tag="VFB37_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21821.1"
                     /translation="MYDDLGATYSALGNPAEAAEAFAKAVSCNPRATYLHAELASEYL
                     HLGRFEDAKAEARRGPQPEGAAFTVNSLLAQAEFSQQQWTQAIQHLRLAAVEAPDEVQ
                     ATYWQCLLWLAQKRAGTATPALASRAPTTLWPRPVLDFLQGTIDETALVHAVAAERDE
                     RRRREILCEALFYTGESYLAGHRGTEARRYFQATVNLQVYDFIEHYLAKAELAR"
     gene            complement(15808..17073)
                     /locus_tag="VFB37_04900"
     CDS             complement(15808..17073)
                     /locus_tag="VFB37_04900"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZO21822.1"
                     /translation="MNVELGGLMAELGRSAVAAAEVLATAEATLKNTMLRASAAAVRA
                     RSASILRANHQDWAAAQEAGLAAPLLDRILLDAPRVEAIARSLEAIAELPDPIGVIAK
                     QWRRPNGLQIQRVSVPLGVIGIIYESRPNVTADAGGLCLKSGNAVILRGGSECRHSNG
                     GIHACLVEGLRASGLPLHSIQLVPTSDRAAVGHMLAGMSEYLDVIVPRGGKSLVQRVR
                     EEARVPVIGHLDGNCHVYVDRDADVGMARTIVLNAKLRRTGICGAAETLLVDRACVST
                     HLAPLVCDLLEAGCEVRGDETVLSVDPRVQAANEEDWRTEYLDAIIAVRIVDGLDGAI
                     AHIGAYGSGHTESIVTENQLTAERFLREVDSAIVLHNASTQFADGGEFGMGAEIGIST
                     DRLHARGPVGVEQLTSYKYVVRGSGQVRA"
     gene            complement(17078..>18157)
                     /gene="proB"
                     /locus_tag="VFB37_04905"
     CDS             complement(17078..>18157)
                     /gene="proB"
                     /locus_tag="VFB37_04905"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZO21823.1"
                     /translation="WLETLIEDLLRLRRRGQQVILVSSGAIALGRRQLGLARGSLRLE
                     ESQAAAAVGQIRLAHAYKELLEPGGVTVAQVLLTLDDTESRRRYLNARATLEALLELG
                     ALPVINENDTVATAEIRYGDNDRLAARVAQMAGADCLVLLSDVEGLFTADPNRRPEAR
                     FIEQVSQITPDIEAMAGASASAVGSGGMATKILAAKIAVAAGCHMGIAAGHPRHPVRR
                     IEEGGRCTWFVPHGTPVAARKQWIAGTLRPAGAITIDAGALRALLEGKSLLPAGVLRS
                     LGRFERGDTVSVLGTDGTEVARGVIAYSDGDAARIMGRRSSEIEAILGYRGRDEMIHR
                     DDLVLLRPQAQAGSEPARSGASAPP"
BASE COUNT         3276 a         6028 c         5731 g         3122 t
ORIGIN      
        1 cctcgatatg aagcgggccg aggactgcga gctgtttgcg actctcattg gccaggccga
       61 tgtctacatc cagaacttcc gtcccggtac agccacccgc ctcggggcgg gccccgagcg
      121 gctgctcgag ctcaatccgc agctcgtcta ctgctccatc agcggcttcg gcgccgatgg
      181 accctacgcc gagcgcccga gctacgactc cgtagcgcag gcgctgagtg gcttcctgag
      241 cgtaaccgtc gattatcgcc ggccgcagtt tctcggcccg gcgctggccg acgccatcac
      301 cgggctgtac gcctctcagg ggattctcgc ggcactcgtg cagcgcggcc gtcgcggtcg
      361 tggctgtcac ctcgagatct cgatgctcga ggcgatggcg catttcgcga tcgagccgtt
      421 cgcggctttc ttcgcgctcg gcgaaacacc cacctcggcg gatcggccgc ggctcgcgca
      481 ggcctacatc gtacacacag ccgatgagcg cctgatcgcc attcacctgt catcactcga
      541 gaagttctgg cgcggtctgg ttgccgcgct cgaggccccg gagctcggga cggatccgcg
      601 cttcaaccca cgagagcgcc gcatcgccga gtacgagacg ttgcgcgcgg agctcgatag
      661 tcgcttcgcg cagcggccgc tcgcgcactg gacggcccgt ttgcacgccg aagacgtgcc
      721 acacgcgccg gtcaatcgca tcaacgacgt tgtgcgggat gcgcaggtcg agcacctcgg
      781 catcgtcgtg ccggtacgca caccgcactc agcgacccat agcgtgcggg cgccgtggca
      841 attcgcgggt cagcgcgcac atgccgtgtc ggctgcaccg ctgcttgatg agcacggcgc
      901 agcgattcgc ggtgcgctcg cgggcagcaa ggcgtggccg gaagcaaccc aagccgccaa
      961 tagctgaagc ggggatatgc acgtgctctt acccaccatg ctggtcggca gttatccgca
     1021 gccggagtgg ctcatcgacc gcgaacgcct tgcggggcgc ttcccgccgc gagtgcgagc
     1081 gcgggagctg tggcgcgtcc ccgagctata tctggagcag gcccaggacg atgccaccat
     1141 cgtggcgatc aaggcacagg aggacgccgg actcgatttc atcaccgatg gggaaatccg
     1201 gcgcgagagc tactcgaatc gcttcgccac cgctctcgag ggcatcgacc tcgacaaccc
     1261 cgggatcgca ttggaccgca gcggccatcc gaatccggtg ccgcgagtgg tcggtgcaat
     1321 acggcgccgc cacgcggtcg gcgtgcgcga tctgcaattc ctgcgcagtc acacccggcg
     1381 gcagatcaag gtcaccgtgc ccggaccctt cacgatggcg cagcaggcgc agatcgactt
     1441 ctatggcggc agtcgcgagc aggcggcgct cgactatgcg acggcggtaa acgaagagat
     1501 ccgcgacctt ttcgaggccg gcgccgacat cgtgcagatc gacgaaccgt acatgcaggc
     1561 acgccccgag gatgcgcgcg cgtacggcct gcgggcgctc aaccgagcac tcgacggagt
     1621 aagcggcatc accgcagtgc acatctgctt cggctatgcg gccatcatcc atacccgacc
     1681 ctccggctat tcattcctgc cggagctcgc cgagtgccac tgcaaccagg tgtcggtcga
     1741 gaccgcgcag tcaaacctcg actgcgcggt cctcgcccaa ctgcgcggca agaagatcat
     1801 cctcggggtg atcgacctgt caacggcgac cgtcgagtca gcggagctca tcgcgacacg
     1861 catccggcgc gccctgccct acgttgatgc gcatgaactg ctactggcac cggactgcgg
     1921 catgaagtac ctgccgcgcg cagcggcatt cggcaagttg caggccatgg tcgccggtgc
     1981 acgaattgtg cgcgcacaac tggagaacga gaacgctgcg cgcgcgcagt cctgaaactt
     2041 cgataatcag ggggaactca catgtcgacc gctacgcgcc gaaacctgtc agtgctggct
     2101 ggcgtcctga gcgtgatctc tttcagcccg gcgagctggg cgcaaagtca acagtcggca
     2161 tcgcccgacg ccgtggctct cgaggaaatc gtggtcaccg cccgcaagcg cgacgagtcc
     2221 tatcaggacg tgccgatcac ggtgagcgtg ttcaccgagc aggagatcca ggcggcgggc
     2281 atcgagtcgc caagagactt catcgccatg gtccccaaca tgacgttggt ggagacgcag
     2341 aacgtcggca actccttcgt caccgtccgc ggcatctccc aggcacgcaa cagcgagcca
     2401 tcggtagccg tgctggtcga tggcgtcctc gaaaccaatc cctacgagtt cagccaggag
     2461 ctgtttgaca ttcggcagat cgaggtattg aaggggccgc aaggcgcact ctacgggcgc
     2521 gatgccatcg gcggtgcgat catcattcgc accgcggacc cgcccgacca cttcgaggcc
     2581 accacgcgcg tcggaatcgg caacggcgtg tcggagaagg cgcagctcgc cctgggcggc
     2641 ccgctcgaca gcgacggcac gctgcgctac cgggcggcgt tcaacttcta caataccgcc
     2701 ggctacatca acaacgccaa tctcgaccgt gacgccgatc cgtatcgtga ctatgccggg
     2761 cgcatccgcc tggtgtggaa gccgggagat gcgttcagtg ctgatctgcg gctggccttc
     2821 gatcgcacgc agacgaccgc ctattacttc gtcatccccc gcagcgatga agccaatccg
     2881 ttcacggatt tctcgacacc gggcgatgcc aacaacacga cctcgccgat ccagaacaac
     2941 aatctcggca ccgatgaccg taacgtgttt gataccgctc tcaagctcga ctacagcccg
     3001 ggatacggga ccgtcacctc gatcaccgac tatgacgaga cgcgcgagat cgacaccggc
     3061 gatgcctacg acttccggcc gatcacgagc tccatcgcct tcaattgccc gagctgcatc
     3121 ggcgcgaacc cgactgccgc gcaaggcggc ccgttcgacg aaagccagag ccagttcatc
     3181 tgggtgcgta cctggagcca ggagctgcgc ttcacctcac cggtcgtcaa cggcttcagc
     3241 tggatcgcgg gcggctactt cgtgcatacc gaccgcttca tctccaccgg caacctggtg
     3301 gaccgcggat tcggggttcc ggcagtctat tacacaccga tcgtggaccc gagcaatcca
     3361 tacgcgacca actccaatgt gaccttcctg gctgatagtc aggccaacaa cgcctgggca
     3421 ctgtttggcg atgccaccta cgacttcagc aaggcgtggg agctcgacct ggcactgcgc
     3481 tacgatgagg accggcgcga gaacacgacc cttacgccga cgccgttctt gccgaccacc
     3541 gccgcctttt atggcgagaa gcgctatgcc acgtttagcg cctcacagcc caaggccacg
     3601 ctgcggttcc gaccgatgga ggattggact ttctacggtg gctggagccg gggtttccgc
     3661 agcgggggtt tcaatcagac cggtgtaggt gaggtcgccc gcgacaacag cgtgctcggt
     3721 gtgaacgatc tcttccaggc ggagatcgcc gacacctggg agatcggcac caaggccgag
     3781 ctgatggatc gacgcctgag cctcgatgcg gatgtcttct acacgcgatc gaagaacggc
     3841 tatttcttcg tcttcatcgc cgccgattcg actcagaacc tcggcaacct cgatgcgacc
     3901 tataaaggtg ccgaactctc tctcactgcc cgaccaaccg atcgcctcga cgtatacgcg
     3961 agcttcggct acaccgcgag ccgcatcacc gcgatggccg acccgaccgt gatcggcaac
     4021 gaggcgcccc tcgtgtctcg caacacggtc aatgcgggtg tgcagtaccg gcagccgctc
     4081 gcaaatgggc ttaacggtac ggtgcgcctc gattaccagg agatcgggcg cacctggtgg
     4141 gagccgtaca atataacctc gcgcgacccg gtgaacctgc ttgatctgcg gcttgggatc
     4201 cagggtgaga agtgggatct gaccgcctgg tccaagaacc tcaccaatac gatctacaat
     4261 gccgagttct cgccgggcaa tgtcgggggc tcggggttcc tgtggcgggc gctgccgcgc
     4321 cgctacgggg tggatttcac ttacaagttc tgagcggaaa ggggaactgc catggcgcag
     4381 accaaacaat tgccgtcgcg gctgcatcac acggcatacg tcacgcggga tcaggagaag
     4441 actcgccagt tctatgaaga ggtcatcggc ctgccgttgc tcgccacctg gtgcgaatcg
     4501 gacgagctgt tcggcaaggt gcgcacctat gtgcatacct tctatgggat cggcgacggc
     4561 ggcgcgctgg ccttcttcca gtttgccgat tcagccgatc aggaggaatt cgggcccaag
     4621 atgccggcca cgcccttcca tcatattgcg ctgcgcgtcg atgcggagac tcaggccgcc
     4681 atagaggggc gcattgccgc ggccggtatc aagcctcccg atacctatgt gctcgagcac
     4741 ggctattgcc gctcggtgta tgtcaccgac cctaacggcc tgatcctcga gttcacgcgt
     4801 gatcaccagg acgtggagaa gatcggccgc gatcggcgcg cggacgcgca tcaagcgctg
     4861 aagcgctggc tggcggggga ccacacctca aacaatactt atcggtgagc gtgtcacgac
     4921 ggtccgcgtg agcccatgcg cgagttcgac gagcagagta ttactcaggc ggtgatcagc
     4981 cgactatccg acgctgatga tccgcgtttc aagcaggtga tgctgtcgtt gatcaggcat
     5041 ctgcatgact tcgtccgtga ggtgcgcctg actgaggccg aatggttttc ggccatccag
     5101 tttctgaccg acaccggcaa gacctgtact gacaaacgtc aggagttcat tctgctatcc
     5161 gacacgctcg gcttgtcggc tctcgtcatt acgctgaatc acccggtgga ggacgggagc
     5221 gtcgaatcga cggtacaggg cccgttctac tgggaaggtg cacccgaact gccgcgcggc
     5281 agcaatctgg ccgaaggagt caaaggtgag ccggctttct attccggacg cgtcctcagc
     5341 actgatggca ctccgcttgc caacgccctg ctcgacatct ggtcgggaga tggtgaaggc
     5401 aagtacgaca tgcagatggc cggcgaagcg tacatgcagg cgcgcggccg gatccgcacc
     5461 gatgccgagg gccgctactg gttccgttcg atccgcccga ccttctatcc ggtacccact
     5521 gacggtcctg taggcaacat gctgcgcagg atggggcggc atccgtatcg ccccggacac
     5581 atccatatga tcgtctcggc ccccggacat cacgcggtga caacccatct gtttgttgcc
     5641 ggcagtccat atctggattc ggacgcggta ttcggcatga aggagtcgct ggtggcacag
     5701 ttcgagcggc atccgcccgg cgctggaccc ggcggggagc ctctggagac acctttctac
     5761 accgtgagct acgacttccg gctgcggccc acggtttagg agcacgccga agaccatgag
     5821 tgcgggcgcc ggccgtatcc tgaccacaca cgtcggcagc ctgccgcgca gccaagctgt
     5881 gacggatgtg ctgttcgcgc gcgagcgcga ggagctgcga gcgaccgacc gtgacgagca
     5941 ggtcattacc gaggcggttg ccgaggttgt acgtcgccag gtcgaggttg gcatcgacct
     6001 cgtgagcgat ggcgagatga gcaagatcag ctacgccacg tacattgcgc gacgtctgaa
     6061 cggattcgct ggcgataccc cacgcgagcc gggtcaagac ctggtggaat ttccaggact
     6121 cctgcgcaag ctcgccgagc gaggctcaac ggccaaatac cggcggccac gttgcgtggc
     6181 tgctgtcacc gtcaaggaca cgaggcccct ggcaactgac gtcgcgaatc tgagtgcggc
     6241 tgtcgcgagc gcacagccgg tgggcatgtt catgaacgcg gcctcccccg gcgtggtcgc
     6301 actctttcag cccaacgact actaccgcac gcaggatgag tatctcgaag ccctcgcagc
     6361 ggctttacag tccgagtacg aggcgatcgt gcaggcgggc atcatcttgc agattgatgc
     6421 cccggatctg gccatgggcc ggcacaccat gtatcgcgat cgcagcatgg aagagttcga
     6481 gtcgcttgcc gcgcgccaca tcgaggtgct caaccatgcg ctccgtaacg tgccggccga
     6541 ccgcgtgcga atgcatgcct gctggggaaa ctacgaggga ccgcatcacc acgacgttcc
     6601 gatggcacgc ctcctgccca tcctgctcaa ggccaaaccg cagggactgc tgttcgaagc
     6661 cgctaacccg cgtcacgccc acgagtgggc ggtgttccaa ggcgctgaca ttcctgaaga
     6721 gaaggtcctg atcccgggag tgctcaccac cacgaccaat tacgtcgaac atcccgagct
     6781 cgtcgcgcag cgcctggagc gctttgcgca gatcgttggg cgtgaccgcg tcatcggggg
     6841 taccgattgt ggattcggca cctttgctgg cttcgggccg gtcgagcccg atatcgcgta
     6901 ccttaaactc agatcactcg tcgaaggggc tgccatcgcc tcagagcggc tgtggcgata
     6961 gcgcgtggca acgtgcactc cgtcgtccac ggcccccaag gtacgctgct gagatgtgat
     7021 tcgcgcgccc ggacggattc gaaccgccga ccctcaggtt cgaagcctga tgctctatcc
     7081 aactgagcta cgggcgcagc caatgccgag tctaccggct cctcgcccgc cagcaagccc
     7141 gctttttcag gccgccttgc tttggccgct cgcccggaaa gcccgcaatc cctcgagaaa
     7201 ggcgtccacc tcgggctccg tgccgattgt gatgcgacac cagttatcga gcggtggaaa
     7261 gcgcctgccg accaggatat tgcgcttgcg catgaactcc gtgatacgcg gcaacggcgc
     7321 acccgtgtcg aagaatacga agttgccctg cggctcggcg tagttgaatc ccatcgagct
     7381 cagcccggag gtgatgcgct tgcggctcgc caggatgcgc cgccgggtat ccgccaggaa
     7441 cgcatggtcc cccaaactgg cacaagcacc ccgcacagca aacacgttag gggtcgtcat
     7501 tcgcgccttg gcgatctcct tggcgacatc cggtcgagcg atggcgtagc cacagcgcac
     7561 gccagccatg ccgtgaatct tggagaaggt cctcaacacg acgacgcgct tgctgcccga
     7621 ggtgagactt gccacggagc gaacctccgg agcgtcggta aaatccagat aggcttcatc
     7681 gacgatcgtc gtcacgtgct caggcatcga gctgacgaat gcctcgatgg ctgaggccgg
     7741 gacgaccgtg cccgtcgggt tgttgggatt gcagatgtaa acagcgcgcg tgcgatcgga
     7801 caccgcggcg tgcatggcgg tgaggtcgtg gcgcagtttg ccgtccacgg gcacccattt
     7861 gagctcggcg ccggacccct gcgcgtactc ggtcagctcc tgataggtcg gctgggcagc
     7921 gacgatctca ccgcccccat gaccacagag catgccgagg gctcgcagca gctctcccga
     7981 accggtcccc gtcacgatgc ggtcctgcgc cacgccctcc ttcttcgcga gcaggtccac
     8041 gagctttcca atctcatcat ccggatagcg gcatccctgc gacaccgcgg ccgtgatcgc
     8101 ggcgcgcgct gcaggcgatg gaccataggg attctcgttc caacacagga tgatcggtgc
     8161 ggcacccgcc tcggcagctg tcgtcgtcgt tgccgccaca gcagtccgcg cctgctgggc
     8221 acgggccagc gcagcacccg gcggtaccag caacaacgcc gaagcggcga gcagttcacg
     8281 gcggctcatg tccatgggtc aacctcaggg aaagtcgtgc ggggcccgta cgctagcatg
     8341 gcatctgcat cggttccaca cgctccatgc ggcgaatccg gctacagtgt ggaggttcaa
     8401 accttgaccc gaaaccgaca tgccggagag aagacgttgc gcatgggcct ccgggctcga
     8461 tgccgactac acccgatacc acgatgagga gtgggggcgg ccggtgcatg acgatcggcg
     8521 cctgttcgag atgctcatcc tggaaggtgc tcaggcggga ctttcctggg cgacgatcct
     8581 gcgcaagcga gctcactatc ggcgagcgtt cgcggatttc gatccggcac gcgttgcgcg
     8641 tttcgatgct cggcggcgcg cggcgttgct gaaggatgcc ggcatcgtac gcaaccgcct
     8701 caaaatcgag tccacggtca gcaatgcccg cgcctttctc gaggtgcagc gcgaattcgg
     8761 ctccttcgac cgctacctgt ggggcttcgt tgccaatcga ccccggatcc gcctggcgac
     8821 cggcaggagg ccgccggcac gaaccgagct cagtgaccgc atttccaaag acctgaagaa
     8881 acgtggattc cgcttcgtgg gcacgaccat cctctacgcc tttctgcagg cagtgggggt
     8941 ggtcaatgac cacacccgcg agtgcttttt gcggccccgc ccgcgccgcc gcagccccgc
     9001 cgccgcccgg gcagtgcggg gtggccgaga gtagccgcgc acgccgcccc gccactaaga
     9061 tagcggttca atcacaatcg gggatgatcc tatgagagta ctcgctgcca tggcgacagt
     9121 cgccgcgctg gcggtcgggc cggtctatgc ggactgcagc tatccgccgc cgccggacaa
     9181 gctgcccgac ggcaacacgg ccaccaagga tgagatgctg gccggtcagc agacggtcaa
     9241 gacctatgac aaggcgatca acgcctacct ggcctgtatc aagctcgagc acgacggagc
     9301 cgcaagcaag atcggcgaca agcccagcac tccggagcag aagaaggcgc tggacgatct
     9361 caatcgcgtc cagattcaga agaacaacgc cgccgtcgat cagctgcaga gcgtcgcaga
     9421 ccgtttcaac gagcaggttc gggcctacaa ggcgaagcac aaggacgatc cgaagggctg
     9481 acccccgtgc tgagtcccgc gcaggctgat gagctgatcg ggcagcacgt gacctgtctg
     9541 ccgatcgaat cgctgcctct cgcgcagtgc gcgggggccg tgctgcggga gaatatctac
     9601 gccgagcggg atcagccgcc cttcgaccgg gtggcgatgg atgggatcgc cctggacagc
     9661 aacacggtcc agcatggaac tcgccggctg cagatccagg ccacgcaggc ggcgggcgat
     9721 ccgccgttag cgctcgcaac acccgcctct tgcatcgagg tgatgaccgg cgcagtggtc
     9781 ccctctggtt gcgacagcgt cgtgccggtt gagcagatca ccgtcgatcg cagcgaggcg
     9841 gagctcacag ccggggtgac cgtggagccc tggcagaacg tccatcggcg cggcaccgac
     9901 acccgccagg gggcccttct gctctccacc gggctgcggc tgcaggctcc ggagatcgcg
     9961 atcgctgcct ccgcgggcat ggcgcgcatc cgggtcagca gccagccgat ggtggcggtg
    10021 atctcgaccg gcaatgagct catcgagcct ggcgagccga tcctgccgca tcaggtccgt
    10081 cgctccaatg cgtacgccat cgttgcggcg ctgcgccggc atggcttcca gcgtgtcgcc
    10141 gacgatcacc tgcttgatga cccggcggag ctcaagcgcc gcctcaagtt ccacctcgag
    10201 acacacgacg tcctggtcat gagcggcggc gtatcgatgg gcaagctcga tttcgtgcct
    10261 cgggtgctcg atgagctcgg cgtgcagacg gtctttcaca agatcatgca gcggccgggc
    10321 ttgccgatat ggttcggtgc cgcgagctcg ggcgccaccg tatttgcgct gcccgggaat
    10381 ccggtctcca cgctgatgtg cctcacccga tacgcgctgc ccgcgctctt cacggccatg
    10441 ggccagcttc cgcccgcgcc ggagcgcatg gcactcggcg gcgaaatcaa ggtcaaatcc
    10501 ccgcatacgc gcttcatgcc agtcaagatc gaggtcgatg actggggccg agcctgggcg
    10561 atgccccggc cgacgaatgg gtcgggggac ttcacctccc tgggcggcac ggacggcttc
    10621 gtcgagctgc ctcccggacc gaataactat ccgaagggct tcgtcacgcg tctgcaccgc
    10681 tggtgaaggc ggcatccagc ccccatatgg gcagatagcc cggcctgatt ggcgtcgcgg
    10741 ctgacgcggt ggtaagacca cttcccgagc cccgacgtag ggccaaccga tcaaggtatt
    10801 atgtccagac ccgaagtcgt cgttccgctc aataatctcg ccggcgtgcg cgacacgctc
    10861 ggccggccgg tgcgcgacct gcgcatctcg gtcatggatc ggtgcaactt ccgctgcccg
    10921 tactgcatgc cgcgcgagaa gtaccacgag cgctacaagt tcctcggttc gcatgagcgt
    10981 ctctcgttcg atgagatcgt gcggctggcg cgcctgttcg tgaaactcgg cgtgcgcaag
    11041 ctgcgtctga ccggcggcga gccgctgctg cggaccaacc tctccgatct catcggggat
    11101 ctcacggcga taccgggcgt ggaggatgtt gcactgacga caaacggggt cctgctggcg
    11161 cgccaggcgg cggagctcaa ggcggcagga ctgcggcgca tcaccgtcag tctcgacagc
    11221 ctcgacccgg agttgtttgc ccggatgagt ggcggcttcg gcggcctcga ggacgtcctc
    11281 gacggaatcg ctcacgcccg cagcgcagga ctcgagccga tcaagatcaa ctgtgtgatt
    11341 cagcgcggca tcaacgatgc gacggcggtg gatctggtgg agcattttcg cggcacgggc
    11401 gtcatcgtcc gcttcatcga gtacatggac gtcggaagcc gcaacgaatg gcgtcaggat
    11461 ctggtggttc cgtcgaaaga gctcgttgcg cgcatctccg agcgctggcc gctgcaggcc
    11521 gtggaacgtg agtatcgcgg tgaagtggct cagcgctatc aatttgccga cggcgccgga
    11581 gaagtcggct tcatctcctc ggtgactcag cccttctgcg gtgattgctc gcgcgcccgc
    11641 ctgtcttccg acggtgtgat ctacacgtgc ctgttcgcaa cgcatggcac gaacctgcgc
    11701 gatgcgctgc gcagcggcgc gagcgacgag cagctgctgc agatcatccg cagcatctgg
    11761 ttggctcgca cggaccgtta cagcgagcaa cgcgctcagt tgcgtaaaac ggccggcgat
    11821 cagcgcaagg tcgagatgtt ctacatcggg ggttgatgtg ccccgcagca gcccaggcga
    11881 cctgactcat ctcgatgccc gcaatcggcc gaccatggtc gatgtgggcg cgaaggatgc
    11941 cactcgacgg atcgcaacgg cggaggcgcg catccgcatt ccgccagcgg tcgcgcaggc
    12001 tctacgcaag agcggccatc ggaccaagaa gggcccggtg ttcgataccg ccattgttgc
    12061 cggagtcatg gcggccaagc gcacccacga gctgattccc ttctgccatc cactcgggct
    12121 ggacaactgc gccatcgaga tcgaggagcg ccgctccggc gccgagctcg tgattcgctg
    12181 tcaggcctct gttcaccatc gcacgggtgt cgagatggag gctctcacgg gtgccaccgt
    12241 ggccgcgttg accgtttacg acatgtgcaa agcgctttcg catgagatcg agatcaccag
    12301 catccggctg ctggagaaaa gcggcggtaa gcgcgacttc cggcgcaaag cgtaacattg
    12361 cacccatgag cgacctctcc tccccggccc cgaccctgta tggactcgtg cttgcgggcg
    12421 gtcgcagctc gcgcatgcag cgtgacaagg ccgagctgag ctttcacggc cgctcgcagc
    12481 tcgaatggac ggtggcgctg ctcgagcctc acgtgcagcg cgtattcgtc tcggtccggc
    12541 cggaccaggt ggatgacccc atccgcgccc gattcgccca ggtcgtggac gagcgcgccg
    12601 ggctcggccc catcgcggga atcctcgccg cccaagcgcg ttatccccgt gccgcctggt
    12661 tggtgctcgc ctgcgatctg ccttacgtgg atcagacgac gatcacgcat ctcatccgcg
    12721 cccgagaacc gcacaagcag gctactgcct accgctccag ctacaacggg cttccggagc
    12781 cgctgtgcgc catcttcgag ccgagcaccc gcgaggcgct gcttgcctac gccggccgcg
    12841 gaaaggactg cccgcgcaag ttcctgatca actccgacat ccagctgctc gatcagccca
    12901 atccccgcgc gctggagaat gtgaacacgc cagccgagcg cgactcggcc atgacggcac
    12961 tgagttcaaa agaggcgagt cccggtcggc cccgggcaag cgggcgcccg gaggttgccc
    13021 tatccatgac gagcaagcga atcaaggttc agtactacgc gttgctgcga gagcaggcgg
    13081 gccgcagcga tgagactctc ctcacgagcg cgcgcacgcc gcgcgagctc tatgaggagt
    13141 tgaaagctcg ttaccccttc tcactcgcac ccgaaatgct gcgtgtagcg gtgaatgccg
    13201 agttcggcga atgggggaac cccctcgcag acggcgattt cgtcgtgttc attcccccgg
    13261 ttgccggcgg ctgaacatgc aacgcgtcgc ctctttccgt ttcagccacg agaagatcga
    13321 tgaggcgtcc ttgcgtgagc agctcgcgga tcctgccgcc ggggcctaca catcgttcga
    13381 aggttgggtg cgcaaccaca atgaaggcct gtcggtccat cacctcgaat acgaggcatt
    13441 cgaaccgctc gctgtcaaag agggcgagcg catcgtcgcc gaagccgtgg cgcgcttcgg
    13501 cgtcgagcac gctgcctgcg tacaccggac tggcgatctg gccataggcg aaatggccgt
    13561 ctgggtcggc gtgagcgccc ctcaccgcca tgaggctttc ctcgcctgtc gctacatcat
    13621 cgacgaggtc aagcatcgcc tgcccatctg gaaaaaggag cattacgaga acggcgattc
    13681 cggatgggtg aattgcgagc ggtgtgccga gcatggcggt gcggagcatg actcttcaca
    13741 gcatcaccat ccaaacactt cgcgtgcgga tcacgtcctg gtgccggact actcgcgtca
    13801 gatggcgctg cgggaagtcg gggccgcggg gcaggccaag ctgcgcgcgg gcaaagtgct
    13861 ggtggtcggt tgcggtggcc tcgccgtgcc ggtagtgggc tatctggccg gtgccggaat
    13921 cggtcgcctg gggttgctcg atagcgatcg actggaaccc tccaacctgc accgtcaaac
    13981 gatgtacgcg ttgagcgatg tcgggcagct gaaagcggag ctggcggcgg agcgtgctcg
    14041 tgccctgaat ccggaggttg aagcgcgcgc tcacgtgctg cggctcgatg cgcagaacgc
    14101 gggcgagctc atcgcgcagt acgatctcgt ggtcgactgc acggataatt tcgcgacgaa
    14161 gttccttctg aatgacttct gcgtgctgtt gaacaagccg gtggtctttg cgagcgtcta
    14221 tcagtacgaa ggtcagttgc aggtcgtgcg cccgctgcgg gatgcagaaa gcgagaaccg
    14281 atcgggcgcc tgcttgcgct gtgtgtggcc ggaggctacc cgcgacggca tcgtgggcaa
    14341 ctgcgccgaa gcaggtgtgc tcggcccggt accgggtgtg ttcggtgcgc tgcaggcttt
    14401 cgaggccctg aaacttctcc tcgacctgcc gggtcagttg accgacgagc tgctcgtgct
    14461 cgatctgctg acgctgtcga ttgcacgtgt gcggactcgg cgcgcggccg cgtgtcccca
    14521 acatgcgatc tctcgagccg agtcggctca gaaagcggcc tcacacgccg cctcggacac
    14581 tgtcccggaa ctggaggtca gcttcgactc gctcgagcag gctctggatg ccggtttcga
    14641 gctggtggac atccgcgaag ccgaggaggt ctcggagacc cccgcgccct cgaggcaggt
    14701 caaacacgtc ccgatggcgc agctgctgta tgggaagacg gagctcgagc cgcgggcgaa
    14761 gagtctcctc atctgcgcca gcggccggcg cagccttgca gccacgcaag aattgcgcgc
    14821 gcgcggactc gagaacgtgt attcccttcg gggtggcgtc acggccctac aggggcgact
    14881 gagcacctga agccggctta gcggctcgag gacagcattc tgagacgtgc gcgtactcta
    14941 gtcctcctgc tcgcgctcgc gacggcgctc ggggcggcca ccgcaccgtc gtctcccctc
    15001 ctgagctacg cagcctcgca gcgactcctg cccctggcag agcaaggcgc gcggatccgc
    15061 cggctggacc agatcacaca cacgatcgag aagaacccgc cgcccgagca agattgcgcg
    15121 cgaacgctcg gggcgcggcg attcgccagc atgtatgacg acctcggcgc gacctattcg
    15181 gctctcggaa atcccgcgga agccgccgag gcctttgcca aggcggtcag ttgcaacccg
    15241 cgcgccacgt atctgcacgc ggagctcgcg agcgaatacc ttcacctggg tcgcttcgag
    15301 gatgcgaagg cggaagcccg ccgcgggccg cagccggagg gggccgcatt cacggtcaat
    15361 tccctgctcg cccaggcgga gttcagccag cagcagtgga cgcaggcgat ccagcatctg
    15421 cggctcgcgg cggtcgaagc tccggatgag gtgcaggcca cgtactggca gtgcctgttg
    15481 tggctggccc agaagcgtgc agggactgcg acaccggcgc tggcttcccg ggcccccacg
    15541 acactgtggc cgcggcccgt actcgacttc ctgcaaggca cgatcgacga gaccgcactg
    15601 gtgcacgccg tcgcggcaga gcgtgacgag cggcgtcggc gcgaaattct ctgcgaagca
    15661 ctgttttaca ccggcgagag ctatcttgcc gggcatcgcg ggaccgaagc gcgccgctac
    15721 ttccaagcca ccgtcaatct gcaggtgtat gacttcatcg agcactacct cgccaaggca
    15781 gagctcgcaa gatgagctcg aaggcggcta cgccctcacc tgcccgctgc cgcgaacgac
    15841 gtacttgtag ctggtgagct gctccacacc taccggaccg cgcgcatgca agcgatccgt
    15901 cgagatgccg atttccgctc ccatgccgaa ctccccgccg tcggcaaact gcgtcgaggc
    15961 attgtgcagc acgatggcac tgtccacctc gcgtaaaaaa cgctccgccg taagctgatt
    16021 ttccgtgacg atcgactcgg tgtgccccga tccataggcg ccgatgtgcg cgatcgctcc
    16081 gtcgagcccg tccacgatgc gcaccgcgat gatggcgtcc agatattcag tccgccaatc
    16141 ctcttcgttc gccgcctgca cacgcggatc gacgctcaga acggtttcat ccccccgcac
    16201 ttcgcacccc gcctccagaa ggtcgcacac caggggcgcg agatgcgtgc tcacacaggc
    16261 acgatcgacc aacagcgtct cggcagcgcc gcagatcccg gtgcggcgca gcttcgcatt
    16321 caacacgatc gtccttgcca tgccgacatc cgcatcccga tccacataga cgtgacagtt
    16381 gccgtccagg tggccgatca ccggcacgcg cgcttcctcc cggacccgct gcacgaggct
    16441 tttgccgccc cgcggcacga tcacgtccag atattcgctc atacccgcca acatatggcc
    16501 gacggcagcc cggtcggagg tcggcaccag ctggatgctg tgcagaggaa gcccactcgc
    16561 gcgcaaaccc tccacgagac acgcatggat gccaccattg gaatgccgac actccgagcc
    16621 gccgcgcagg atgaccgcgt tgcccgactt gaggcacagt ccgcccgcgt cggccgtcac
    16681 gttgggacga ctttcataga tgatgccgat caccccgagc ggcacgctca cccgctgaat
    16741 ctgcagtccg ttggggcgcc tccattgttt ggcaatcacc ccgatcggat cgggcagctc
    16801 tgcgatcgcc tcgagactgc gggcgattgc ttccacgcgt ggggcatcga gcaggatccg
    16861 atccaggagc ggtgcggcga gacccgcctc ttgcgcagcg gcccagtcct gatgattcgc
    16921 gcgcaggatg ctcgcgctac gcgcgcgcac cgcggcagcc gacgcgcgca gcattgtgtt
    16981 cttgagtgtc gcctcggctg tcgcgaggac ctcggctgcg gcaacggccg agcggccgag
    17041 ctctgccatc agcccaccga gctcgacgtt catccggtca cggcggagcg ctggcgccgg
    17101 agcgcgccgg ctcggatccg gcttgagcct gtggccgcag cagcaccaga tcatcgcgat
    17161 gaatcatctc gtcacggccg cgatagccca ggatggcctc gatctcagac gagcggcgac
    17221 ccatgatccg ggcggcatcc ccatcggagt aggcgatgac tccgcgtgcc acttccgttc
    17281 catccgtacc taacacgctg accgtgtccc cgcgctcgaa tcggcccagt gagcgcagca
    17341 ctccagcggg taagaggctc ttgccttcga gcagggcgcg caatgcaccc gcgtcgatgg
    17401 tgatggcacc cgcgggacgg agcgtcccag cgatccactg cttgcgcgcc gcaacgggcg
    17461 ttccatgcgg cacaaaccag gtgcatcgtc cgccctcctc gatccgccgc acgggatgcc
    17521 tgggatggcc ggccgcaatg cccatgtggc atcccgccgc cacggcgatc ttcgcggcca
    17581 ggatcttggt agccatgccc cctgagccga ccgcagaggc cgaggcgccg gccatcgcct
    17641 cgatgtcggg cgtgatctgg ctgacctgct cgatgaaacg agcctcggga cggcggttgg
    17701 gatcggccgt gaagagacct tccacatccg acagcagcac caggcagtcg gcccctgcca
    17761 tctgcgccac gcgcgctgcg agccggtcgt tatccccata acggatctcg gccgtggcaa
    17821 cggtatcgtt ctcgttgatg acgggcagtg cgccgagctc cagcagcgct tcgagagtgg
    17881 cgcgcgcgtt gagatagcgg cgacggctct cggtgtcatc cagtgtgagg agcacctgtg
    17941 cgaccgtcac gccaccgggc tccaacagct ccttgtacgc gtgcgccagc cggatctgcc
    18001 cgaccgccgc ggcggcctgg ctctcctcca accgcaggga accgcgggcg agcccgagct
    18061 ggcgccgtcc gagtgcgatg gccccggagg acacgaggat cacctgctgg ccgcggcggc
    18121 gcagccgcaa tagatcctcg atcagtgttt ccagcca
//
LOCUS       DASRHN010000127         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1195232, whole genome shotgun sequence.
ACCESSION   DASRHN010000127 DASRHN010000000
VERSION     DASRHN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195232"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..726)
                     /locus_tag="VFB37_04910"
     CDS             complement(49..726)
                     /locus_tag="VFB37_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21824.1"
                     /translation="MALNLSELTKERREEVQADMRKTGTFQQAWLWLAVFFCAGACLL
                     SACGNADNPKSATSSYNAKARKAQKPVHSTKPGEEDLTEMVAAVSATKAGPPVEMRFA
                     LQQSPQVGQPVAVSIALVTSAPALDSVSASFQASDGLDIVEGAESPRVDRPVPGTPLR
                     HVLRIVPKRDGIFAVTAVVTVDSSNQHSTRTFLIPVIAGEGLSEPAKSAEGSSTPPAR
                     PPDATKG"
     gene            complement(840..1850)
                     /locus_tag="VFB37_04915"
     CDS             complement(840..1850)
                     /locus_tag="VFB37_04915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZO21825.1"
                     /translation="MREMINKSRAADVKPFTGLSPDVVLDAAASVGLEVDGRLFALNS
                     YENRVYQLGAAPGSLVLKFYRPQRWSDEQIAEEHQFTAELAAGELPVAAPIVVGGETL
                     FRYKEFRFAAFPWMRGRAPELDATDSRQILGRSLARLHQIGSLRSFRVRAHLGIQRLG
                     VEASEQVLEGELLPDVLRDRYAQTSEALIDRITQAFEEAGELREVRIHGDCHLGNLLW
                     NEHGPAFVDLDDCTTGPRIQDLWMLLAGSASDQRRQWAQLLEGYLQFADFDFREVALI
                     EPLRALRMLHHAAWVSHRWRDPAFPRAFPWFGEHRYWEGYLGDLREQLGTIEDPPLLG
                     GA"
     gene            1849..2841
                     /gene="galE"
                     /locus_tag="VFB37_04920"
     CDS             1849..2841
                     /gene="galE"
                     /locus_tag="VFB37_04920"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZO21826.1"
                     /translation="MKRASILVTGGAGYIGSHVVLQLRARGERVIVLDDLSTGFRQAV
                     LDTPLVVGQVGDREQVLQVLQSHEVDTVLHFAAHTIVPESVSEPLKYYGNNTCSTRNL
                     LQCCVETGVRHFVFSSTAAVYGIPAESFAAENTPTAPINPYGTSKLMSEWMLRDVAAA
                     SSLRYVALRYFNVAGSDTAGRIGQATRKATLLVKVACEAAVGKRPHVSIFGTDYPTSD
                     GTGIRDYIHVEDLATAHVDALSYLQAGGNSAVLNVGYGHGYSVREVLASVERVAGQAL
                     KVREEPRRPGDPPTLVARADRVRSELGWKPRLDDLDTIVRTSLEWEKRLQRAPW"
     gene            2900..>4341
                     /locus_tag="VFB37_04925"
     CDS             2900..>4341
                     /locus_tag="VFB37_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO21827.1"
                     /translation="MSGMQWARPKSLSGLMLLGLALIAVPLLAAVLTAALQIRALADT
                     GQKIVVEGVTAARASQELFSQIASLERTARLYDVLADRKLLDLYRQQNDHLSATRAQL
                     EGHEQAAPSARGTLRELGELQRNIGESVLSMQAGKGANTTGLSRRFTELSALADRIAA
                     QSNAQIDAEVGALERQTQQARARLLWQAALLLPLTIIAIVVLTLVVGRPLRQLDRAIS
                     ELGQGAFSNPIVVAGPHDLERLGGQLEWLRQRLLDLALERNRFLRHMSHELKTPLANI
                     REGTELLMDGAVGELDSNQREVTAILRENGIKLQRMIENLLSFSAWQTSSVGLETSEF
                     RLRPVVKQVLENQQLTLLSQRVRLDVQVQDVTLIADRSKIRLILENLVSNAVKYSPKG
                     GTIYLRARTEGALLRLDVADSGPGIPLEDRAHVFEAFYTGRASKNTAIKGTGIGLSVV
                     LEFVSAHGGTVEIRDGEFPGAHFRISMPVQA"
BASE COUNT          769 a         1449 c         1390 g          733 t
ORIGIN      
        1 gtcgtcttat ttgacataag cgtccacgtg ccgccccggg ggccggattt agcccttggt
       61 cgcgtcgggt ggtcgggccg ggggtgttga agagccttcg gccgacttgg cgggctcgga
      121 caacccctcg cccgcaatga cgggaatcaa gaaagttcgg gtgctgtgct gattagagga
      181 atccactgtc acgacggcgg tgacggcaaa gatgccatcg cgcttgggaa cgattcgcag
      241 cacgtgccgc agtggcgtgc cgggcacggg tcgatcgaca cgcggggact cggcgccctc
      301 cacgatatcc agtccgtcgc tcgcctgaaa cgaggccgag acgctgtcca gagccggtgc
      361 ggaggtcacc agcgcaatcg aaaccgccac gggctggccg acctgcggac tctgctgcag
      421 tgcaaacctc atctccaccg gaggccctgc cttcgtggca ctcaccgccg cgaccatctc
      481 cgtcaggtcc tcctcgccag gcttggtgga gtggacgggc ttctgcgcct tgcgcgcctt
      541 cgcgttatag gagctggtgg cggatttcgg attgtcggca ttgccacagg cgctgagcag
      601 gcaggcgccg gcgcagaaga acacggcgag ccacagccac gcttgctgaa aagtcccagt
      661 ttttcgcata tctgcctgca cttcttcgcg tcgctccttg gtcagctcgg acaaatttaa
      721 cgccatcgcc gtccccaacg cacgcgcgag atgaccattt cgtgaagcgt tttacgcctg
      781 cgccaaggcg acagctgcag ctcgggcgcc tcggacgaag gatcttgcca tgccgcgcct
      841 catgcgcccc ccagcaacgg ggggtcctcg atcgtgccga gctgctcgcg cagatcgcca
      901 agatagccct cccaataccg atgttccccg aaccagggaa aagcccgcgg aaaggcaggg
      961 tcgcgccagc gatgcgacac ccaggcggca tgatgcagca tgcgcaaggc tcgcaagggc
     1021 tcgatcagcg cgacctcccg gaaatcaaaa tccgcgaact gcaggtagcc ttccagaagc
     1081 tgcgcccatt gccgccgctg atccgaggcc gagccggcga gcagcatcca cagatcctgg
     1141 atgcgtgggc ccgtcgtgca gtcatcgaga tccacgaacg ccggtccgtg ctcattccag
     1201 agcagattgc cgaggtgaca gtctccatga atgcgcacct cgcgcagctc accggcctcc
     1261 tcgaaggcct gcgtgatgcg gtcaatcagc gcctcgctcg tctgggcata gcggtcgcga
     1321 agcacatccg gcagcagctc gccctcgagc acctgctcgc tcgcttccac tcccaggcgc
     1381 tggataccca ggtgcgcacg cacgcggaat gagcgcaggg agccgatctg atgcagccgc
     1441 gcaaggcttc tgccgaggat ctgccgggag tcggtggcat cgagctcggg ggcccggccg
     1501 cgcatccagg ggaaggccgc aaagcggaat tccttgtacc gaaacagtgt ctcaccgccc
     1561 accacgatgg gggcggcgac cggcagctcg ccggcggcca gctcggcggt gaactggtgc
     1621 tcctcagcga tctgctcatc gctccagcgc tgcgggcggt agaacttcag caccaggctg
     1681 cccggcgcag cgccgagttg atacacacga ttctcgtagc tgttcagggc gaacagccgc
     1741 ccatcgacct ccagcccgac ggaggcggcc gcatcgagca ccacatccgg tgacagcccg
     1801 gtgaacggct tcacatccgc agcgcgactt ttgtttatca tttcacgcat gaaacgcgca
     1861 tccatcctcg tcaccggcgg cgccggctac atcggcagtc acgtcgtgct tcaactgcgc
     1921 gcccgaggcg agcgcgtcat cgtgctcgat gacctttcga ccggctttcg tcaggccgtc
     1981 ctcgacaccc cgctggtggt cggccaggtg ggcgaccgcg agcaggtgct gcaggtcctg
     2041 cagtcgcacg aggtggacac ggtcctgcac ttcgccgccc atacgatcgt cccggagtcc
     2101 gtgagcgagc cgctcaaata ttacgggaat aacacctgct cgacgcgcaa tctcctgcag
     2161 tgctgcgtgg agaccggcgt ccggcacttc gtcttctcct cgaccgcagc cgtctacggc
     2221 attccggccg agagcttcgc cgcggaaaat acccccacgg ccccgatcaa cccgtacggc
     2281 acttcgaagc tgatgtccga atggatgctg cgtgatgtag cggccgcgag ctcgttgcgc
     2341 tacgtcgctt tgcgctattt caatgtagcg ggatcggata cggccgggcg catcggtcag
     2401 gcgacgcgca aggccaccct gctggtcaag gttgcctgtg aggcggccgt cggcaagcga
     2461 cctcacgtct cgatcttcgg caccgactat ccaacatcgg acggcaccgg tatccgggac
     2521 tacatccacg tggaggatct ggccaccgcg cacgtggacg ccctttccta cctgcaggcc
     2581 gggggcaact cggcggtgct caacgtcgga tacggacacg gttatagcgt tcgagaggtg
     2641 ctggccagcg tggagcgagt ggccggccaa gccctgaaag tgcgcgagga gccgcgccgc
     2701 cccggtgatc cgccgaccct cgtggctcga gccgaccggg ttcgctcgga gctcggctgg
     2761 aagccgcggc tcgatgatct ggacaccatc gtgcgcacct cgctcgagtg ggagaaacga
     2821 ctgcagcgcg caccttggta gcccgcatgt cagttggtat gccccgtccc cgtggacagg
     2881 gtgccccaag gccctgagaa tgtcaggcat gcagtgggca cgacccaagt cattgagcgg
     2941 attgatgcta ctcggcctgg cgctgatcgc cgtgccgctg ctggccgccg ttctgacggc
     3001 cgccctgcag atccgcgccc tcgccgatac cggccagaag atcgtcgtcg agggcgtcac
     3061 cgccgcccga gccagccagg agctcttcag tcagatcgcc tcgctcgagc gcacggcacg
     3121 cctgtatgac gtgctcgccg atcggaaact gctcgacctg tatcggcagc agaacgatca
     3181 cctctccgcg acgcgcgccc agctcgaggg tcatgagcag gcggcgccct ctgctcgcgg
     3241 gacgctgcgc gagctcggtg agctgcaacg gaacatcggc gagtccgtcc tgtccatgca
     3301 ggctggcaag ggagcgaata caaccgggct gtcacgccgc ttcactgagt tgagcgcgct
     3361 ggcagatcgc atcgccgcgc aaagcaatgc gcagatcgat gccgaggtcg gcgctttgga
     3421 gcgccagacg cagcaggcgc gcgcgcgtct gctgtggcaa gcggcactgc tgctgccgct
     3481 gacgatcatc gccatcgtgg tgctgacact ggtggtcggg cggccgctgc gccagctcga
     3541 ccgcgcgatc agcgagctgg ggcagggagc cttctcgaac ccgatcgtcg tggccggacc
     3601 tcacgacctg gagaggctcg gaggacagct cgagtggctg cgtcagcggc tgctcgatct
     3661 cgcactggag cgcaaccgct ttttgaggca catgtcacac gagctcaaga caccgctcgc
     3721 caacatccgc gaaggcaccg agctgctgat ggacggcgcg gtgggcgagc tcgattccaa
     3781 ccagcgggag gtgacggcca ttctgcggga gaacggcatc aagctgcagc gcatgatcga
     3841 gaacctgctc tccttcagcg cctggcagac ctcgagcgtg gggttggaga ccagcgagtt
     3901 ccggctgcgg cccgtggtga agcaggtgct cgagaaccag cagctcacgc tgctttcgca
     3961 gcgcgtgcgc ctcgatgtcc aggtccagga tgtgacgctg atcgcggatc gcagcaagat
     4021 tcggctcata ctcgaaaacc tcgtgtcgaa cgcggtgaag tactcaccca aggggggtac
     4081 catctatctg cgcgcacgca ccgagggcgc cttgctccgg ctggatgtcg ccgacagcgg
     4141 cccgggtatt cccctcgagg accgggcgca tgtcttcgaa gccttctaca ccggacgagc
     4201 ctcgaaaaac acggccatca agggcaccgg catcggcctg tcggtcgtgc tcgagttcgt
     4261 ctccgcgcat ggcggcacgg tggagatccg cgacggagag tttcccggtg ctcacttccg
     4321 catcagcatg cccgtgcagg c
//
LOCUS       DASRHN010000128         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_532916, whole genome shotgun sequence.
ACCESSION   DASRHN010000128 DASRHN010000000
VERSION     DASRHN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532916"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..967)
                     /locus_tag="VFB37_04930"
     CDS             complement(317..967)
                     /locus_tag="VFB37_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21828.1"
                     /translation="MKPARIIIADDHEFVRRGLVATLAEVRGWTVVAEASNGRKAVDL
                     VREFHPDIAILDLSMPELNGLDATRQILEIDAVTRVLILSAHESEQLTREVLKAGAQG
                     YVLKSDAGRVLVTAVEALLDGRPFFTSKIGQLVLDGYLRGAPVETDSSMPALSSRERE
                     IVQLLAEGRSNKEVARVLGISVKTAETHRSNIMRKMEFGSLADLIRFAVRNKIIDA"
     gene            complement(971..>2286)
                     /locus_tag="VFB37_04935"
     CDS             complement(971..>2286)
                     /locus_tag="VFB37_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZO21829.1"
                     /translation="EADAARGGRMQRQSEALVRLASRNDATWTELRARLRLICETSVD
                     VLAAERVCIWLLDDDLRTLRCAADCGSRPGRPREGATIATSRLSSYLAALRSERVLEA
                     TDAPSDVRLSELLQAGLAWPGARSSIASAVRSSGELSGFVLFESCEQPRVWAPDEVSF
                     AGGVADQVTQALLDSQREQVLKDLRSLAAELMRIQDEERRRIGRDLHDSTGQTLAALE
                     MDLTRLMLNARSLAPKEQDLLAECVRLAGLCSAEIRTASYLLHPPLLDELGLVSALRW
                     LADGLRQRGVIEMRLELPESIPRLRPEEELTLFRVAQEALTNAHRHSASPWIAMRLKA
                     EPDTVQLEIEDAGRGLGAEDDPSAEDAGSAAGRVLDDGDAQAGAPRTADGGMRLGVGL
                     AGMRERIRQVGGTFVVESTGSGTRIRAVLPLHWREERDAQDARSA"
BASE COUNT          392 a          793 c          711 g          390 t
ORIGIN      
        1 caccgtcagc ctggtcacct tgcggctccc tcgacccggg cggcgatcct gtgtagcgcc
       61 gcgctgcgag tcgtacttgg atttattggg ggagcgacgt tagtcgaggc gcgcgcgcgc
      121 gggcattgag gaaattcggt agcgctacca ggggtgattg tgtagtcgcg cagccgccgc
      181 ctgatagctg gtatcaggat ttctgttggt gggttgtcag cgcgctacgc agattaacgg
      241 cggctgaacg cttgcggctt ggctactgtg cagtgcgaca tggagattaa cgcagcgtga
      301 atcgaatctc aaacactcac gcatcgatga tcttgttgcg caccgcaaaa cggatcaggt
      361 cggcgagcga gccgaactcc atcttgcgca tgatgttgct gcggtgcgtc tcagccgttt
      421 tcacgctgat gccgagcacg cgcgcgactt ccttgttgct gcgtccttcc gcgagcaact
      481 gaacgatttc ccgctctcgc gaggacaacg cgggcatcga cgaatccgtc tccaccgggg
      541 cgccgcgaag ataaccatcc agaacgagtt gcccgatctt cgatgtgaag aagggccgtc
      601 catccagaag agcctcgacg gcggtgacca gaacgcgccc ggcatcggac ttcagcacat
      661 agccttgcgc gcccgctttg agcacctcgc gagtcaactg ctcggactcg tgcgccgaga
      721 gaatcaacac gcgagtcacc gcgtcgatct cgagaatctg ccgcgtcgcg tccaacccgt
      781 tgagctcggg catcgacaga tcgaggatgg cgatgtcagg gtggaactcc ctgacgagat
      841 ccacggcctt gcggccgttg gaagcttccg ccacgacggt ccagccgcga acttcggcga
      901 gcgtcgccac gagaccccgc cgcacgaact cgtgatcgtc ggcgatgatg attcgagccg
      961 gcttcacctt ctaggcgctc cgcgcatcct gtgcatcccg ttcttcgcgc cagtgcaggg
     1021 gcagcaccgc acggatgcgc gtgcccgaac ccgtcgactc cacgacaaat gtaccgccaa
     1081 cctgccggat acgctcgcgc attccggcga gccccacgcc caggcgcatt ccgccatcgg
     1141 ccgttcgcgg cgcgccggcc tgcgcgtcac catcgtccag aacacgcccg gcagcggaac
     1201 cggcgtcctc tgccgatggg tcgtcctcgg cgccgagccc gcgccccgca tcctcaattt
     1261 caagctgcac ggtatccggc tcggccttaa ggcgcatcgc aatccatgga ctcgcggaat
     1321 gccgatgcgc gttcgtcagc gcctcctgcg ccactcggaa cagcgtgagt tcctcttccg
     1381 gacgcagccg cggaatggac tccggcaact ccagtcgcat ctcgatgacc ccgcgctgcc
     1441 gcagcccatc ggcaagccag cgcagcgcgc tgacgagtcc cagctcatcg agcagtggcg
     1501 gatgcaacag ataggaggcc gtgcgaatct ctgcggagca cagccccgcg agccgcacgc
     1561 actcggccag cagatcctgc tccttcggcg cgagagaccg ggcattgagc atcaggcgcg
     1621 tcaggtccat ttcgagcgcg gcgagcgtct ggcccgtcga gtcgtgcaga tcgcggccga
     1681 tccggcgccg ctcctcatcc tggattcgca tcagctcggc cgccagcgag cgcaggtcct
     1741 tcagcacctg ctcgcgctgc gagtcgagca gcgcctgggt gacctggtcg gcgactcccc
     1801 cggcgaagct cacctcgtcc ggcgcccaga cccggggttg ctcgcagctc tcgaacaaca
     1861 cgaatccgct gagctctccg gagctgcgca cggcggaagc gatgctggac ctggcgcccg
     1921 gccaggccag accggcctgc agcagctcgc tcaaccggac atcggagggc gcgtcggtcg
     1981 cctcgagcac tctctcgctg cgtagcgcag ccaggtacga ggacaagcgg ctcgtggcga
     2041 tcgtggcgcc ttcgcgaggg cgaccgggcc tggacccgca atcggcggcg cagcgcaggg
     2101 tccgcaggtc atcatccaaa agccagatgc acacccgctc ggcagccaac acgtccaccg
     2161 atgtttcgca gatcagtctc agtctggcgc gcagctcggt ccacgtcgca tcgttgcggc
     2221 tggccagtct gacgagcgct tccgactgcc gctgcatgcg gccgcctctg gcggcatcgg
     2281 cttcct
//
LOCUS       DASRHN010000129        10563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_709796, whole genome shotgun sequence.
ACCESSION   DASRHN010000129 DASRHN010000000
VERSION     DASRHN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10563
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709796"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFB37_04940"
     CDS             complement(<1..656)
                     /locus_tag="VFB37_04940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO21830.1"
                     /translation="MSSIAARPILIMAGGTGGHVFPALALARLLRARSYEVIWLGTRR
                     GLEARVVPAEQIPIEWLSVGGLRGKGLGTLIAAPFRLALALTQALGVMWRHRPLVVVG
                     LGGFVTGPGGVAAWLTRRPLLIHEQNAVAGFTNRLLSHIAREVLEAFPGSFGRGVKAR
                     AIGNPVRQDISALAPPANRFAARGGPIRVLVIGGSQGATRLNAVVPFALARLKQLANG
                     "
     gene            complement(653..1909)
                     /gene="ftsW"
                     /locus_tag="VFB37_04945"
     CDS             complement(653..1909)
                     /gene="ftsW"
                     /locus_tag="VFB37_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZO21831.1"
                     /translation="MNSTLAGSSMTYARSGRGAGVVHIDTVTVALVLAISLLGLIMVT
                     SASVSIASQDTGQPFYYLVRQLVLMLIGGGCAALLFCVRTELLEKASFALLIFAIVLL
                     AVVFVPGLGHTVNGSRRWIHVAGINFQASELARVLVLVYVASYAVRRQEELRSSFAGL
                     LKPLGLLVFVAALLLAEPDFGAASVLFATGFGILFLAGARLRYVIAMTAMAAAGFGLL
                     AVTSSYRMRRLTAFLNPWADPYNTGFQLTQSLIAIGRGQWFGVGLGDSVQKLFYLPEA
                     HTDFLFAVLAEELGLAGVILTLALFLALIWRSFYIARLASEVGLKFQSYLAAAFGLWV
                     GIQAFINIGVNMGALPTKGLTLPLMSYGRSSLIVSLAWVGLLLRVYHEAMLEKRGSAT
                     VRQRGAVMGGSRGSARTAGPTAEELS"
     gene            complement(1906..3261)
                     /gene="murD"
                     /locus_tag="VFB37_04950"
     CDS             complement(1906..3261)
                     /gene="murD"
                     /locus_tag="VFB37_04950"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZO21832.1"
                     /translation="MSTPAAKAPCAVIVGLGRTGLSSARHLRRLGWRVAVTDTREQPP
                     ELLALRELDPRIPLRTGGLDTSLLDGAVCVVASPGVSLAAPFFAEARRRGLEIIGDIE
                     LFARAADAPVVGITGTNGKSTVTTLVGRMAARAGVNVRVGGNLGEPALDLLSAEGAPR
                     RPDLYVLELSSFQLEATYSLALKAATVLNVTPDHLDRYASIEEYANAKARIFARCDTA
                     VINLDDPLVVAMPHEGQRTLSFSLRAAIGADYSISITGADCWLMRRTEALLPISAMRI
                     KGMHNAANALAALALGEALELPMAAMLTELKEFPGLPHRSQWVADVRGVAYINDSKGT
                     NVGATIAAVGGMSGPLIMIAGGDGKNQDFAPLAAAFRGKVRHTVLIGRDAGAIENALQ
                     GICTTETCTSLQEAVRAAARAAKPGDTVLLSPACASLDMFRDYTQRGAVFSEAVRELA
                     A"
     gene            complement(3258..4343)
                     /gene="mraY"
                     /locus_tag="VFB37_04955"
     CDS             complement(3258..4343)
                     /gene="mraY"
                     /locus_tag="VFB37_04955"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZO21833.1"
                     /translation="MLYWVTQQLVGWRSGFNVFSYLTFRAILATVSALALSLLVGPPL
                     IARLSRYQIGQVVRDDGPQTHLLKAGTPTMGGTLILVTTLISTLLWAELTDRLVWMVL
                     GVTFAFGLIGFYDDYLKLVIRNPKGLIARWKYFWQSVAGLSTAGTLYYTATSSSETTL
                     FLPFVKSFAEPLSAFTYITIAYFMIVGMSNAVNLTDGLDGLAIMPATLVIAALGIFAY
                     ASGNAVFAHYLQIPEVRGAGELIIFCGALAGAGLGFLWFNSYPAQVFMGDVGALAIGA
                     SIGVIAVIVRQEIVALIMGGLFVAETASVILQVGSFKLRGKRIFKMAPIHHHFELKGW
                     AEPKVIVRFWIISLLLVLLGLATLKLR"
     gene            complement(4344..5714)
                     /gene="murF"
                     /locus_tag="VFB37_04960"
     CDS             complement(4344..5714)
                     /gene="murF"
                     /locus_tag="VFB37_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZO21834.1"
                     /translation="MMRSLSDFARACDGTLEGEDGPYEAVWSDSRTLSRGDLFIALHG
                     PQHNGNEFVGAALAAGAAGALVDTRQPVALPQILVADTQSALERAASVWRAQYTIPIV
                     GVAGSNGKTTAKEMTAAILAEAGDCLATRGNLNNHIGVPLTTLRIEPHHRFAVIEMGA
                     NRAGEVAALVRIARPTVGLITNAGVEHLEGFGSLEGVARAEGEMVAGLDSSATAVINA
                     DDEFADLWRALTPARITTFGMRKPADFSAIEVQTTVGSEGFITRFTLLTPTGAAGVEL
                     KMGGRHNVANALAAAAAAMAAGAHLSHVVSGLGAVRSVPGRLQFKMTRDGAWLIDDSY
                     NANPSSVRAGIEVLAGLDGHKWLVLGDMAELGEFAESAHSEIGSFAREQGVERLFATG
                     ALAALAARSFGEGAEWFEETGALTQALRGASAEVRMLIKGSRMNRLERVVEALVGPTD
                     NPEGSH"
     gene            complement(5711..7240)
                     /locus_tag="VFB37_04965"
     CDS             complement(5711..7240)
                     /locus_tag="VFB37_04965"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZO21835.1"
                     /translation="MSAAAHKLADLTAGLAVVPSDLRISDVTLDSRAASSGSLFLACQ
                     GRASHGLEFAPEAVARGASAVLYESAAPGEPARRIELLAEQLGKAIFVAAVPQLSRHV
                     GEIADRFFAAPSAALTIAGITGTNGKTTCAWLLAQALQHSGRPAAYMGTLGYGVPPQV
                     TPTQHTTPDAVSVHRQLAALKELGAECVCMEVSSHAIDQDRVGGVRFNTAAFTNLTRD
                     HLDYHGTMQAYGAAKARLFEWPSLAIRIINIDDPFGANLAHQTSRARLIVTSRAAAAH
                     DSVPALAGYVRATRTQPEAAGMTIGVDSSWGEVELKVPLVGEFNVDNVLTVLAVLLAW
                     NIPLAQARLALAQCRAPSGRMELFGGQGTAPLAIVDYAHTPDALAKALQGARLHCRGR
                     LRVVFGCGGDRDVGKRPVMGRIATELADEVIVTDDNPRTEDPGRIVADILNGMPDRTA
                     ALVEHDRARAIQLALRRSAAADVVLIAGKGHEDYQIHGHVRRAFSDQAIVESELAGAR
                     P"
     gene            complement(7237..8988)
                     /locus_tag="VFB37_04970"
     CDS             complement(7237..8988)
                     /locus_tag="VFB37_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZO21836.1"
                     /translation="MKARNTREGSPLSFRWRAYLLIGLLGCCACGLLWRAVNLQLVDH
                     SFLAKEGDARFSRVAEIAAHRGTITDRYGEPLAVSTPVDSIWVNPKELALATDQIPRL
                     ASALKQDRQELTRRITSNLDREFLYLARGRQPSEAEQIKALGIPGVYLSREYRRYYPA
                     GEVAGHILGFTNVDDAGQEGLELAFDHWLAGENGAKRVIQDRYGRIVQDVESIRPARP
                     GRDLVLSLDLRIQYLAYRELKSAIREQRAKAGSIVVLDVTTGEVLAMVNQPAYNPNDR
                     DQILPDLYRNRAATDILEPGSSMKPFFVAAAIESGKYDDHSIIDTSPGFYKVGVKIFE
                     DEHNLGAVNIATVLAKSSNVGMARLALSLEPKLIWSTLTRFGFGQVTTSGYPGESAGL
                     LANYSQWRPVGIATMSHGYGISVTPLQLAHAYATVGSFGISKPISFVRVDTPPAGQRV
                     MDEHGARELVGMLESVVIAEGATGKRAAIPGYRVSGKTGTAYKIISGGYSTDRYMAVF
                     GGVAPASAPRLAAVVVIDEPSAGLHQGGQVSAPVFSAVVGGALRLLAVAPDRPVNAPD
                     ELPPSPTDTQVRTAALR"
     gene            complement(8985..9269)
                     /gene="ftsL"
                     /locus_tag="VFB37_04975"
     CDS             complement(8985..9269)
                     /gene="ftsL"
                     /locus_tag="VFB37_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZO21837.1"
                     /translation="MSSLWRRIFLVALPLLWLAALGSAAGAVYCKHRARELFVELERL
                     SARRDNLEIEWGQLQLEQSAWSTHAFVERVASTKLRMAMPPPKEIEIVAP"
     gene            complement(9266..10195)
                     /gene="rsmH"
                     /locus_tag="VFB37_04980"
     CDS             complement(9266..10195)
                     /gene="rsmH"
                     /locus_tag="VFB37_04980"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZO21838.1"
                     /translation="MDEHSPVLVDEALAALALEAGGYYVDATFGRGGHTAHILEGLGS
                     QGRLLAIDRDPQAIAAGARRFADEVRLTLLHASFADLAALVPAHSRGQPCHGVLFDLG
                     VSSPQLEDPERGFSFRADGPLDMRMDPTRGEPVSAWLARARLDEIREVIATLGEERFA
                     ARVARAIVAEREQRPLTRTAQLAALVAKAVRTREPGKNPATRTFQALRMFINDELGQL
                     EAGLAAALEVLAPGGRLAVISFHSLEDRLVKRFLQRESQLDPQLAALPILPPSVAPPR
                     LRLIGRKTRPAEQEIQRNPRSRSALLRIAEKLT"
     gene            complement(10354..>10563)
                     /locus_tag="VFB37_04985"
     CDS             complement(10354..>10563)
                     /locus_tag="VFB37_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21839.1"
                     /translation="DVEIDGHGRVLLPPELREFAQLERHGMLIGQGNRFELWDETRWG
                     ERRDQWLKSEEAATDLPSELDSLSL"
BASE COUNT         1899 a         3580 c         3479 g         1605 t
ORIGIN      
        1 ctgccattgg caagctgctt caaacgtgcg agagcgaagg gcacgaccgc attcagacgg
       61 gttgccccct ggctgccacc gatgacgagc acgcggatgg ggccgccgcg cgcggcgaac
      121 cggttcgcgg gcggggcgag cgcactgatg tcctggcgca cggggttgcc gatcgcgcgc
      181 gccttcacgc cgcgtccaaa gcttccggga aacgcttcca gcacctcgcg ggcgatatgg
      241 gatagcagac ggttggtgaa gcccgccacc gcattctgtt cgtgtatcaa cagcgggcgc
      301 cgcgtcagcc acgcggcaac gcctcccggc ccggtcacaa agccgcccag accgacgaca
      361 accagcggcc gatggcgcca catcacgcct aacgcctgcg tgagtgccaa cgcgagccga
      421 aagggagcgg caatcagagt tcccaacccc ttcccgcgca aaccccccac cgacaaccac
      481 tcaatcggga tctgctccgc cggaacgacc cgtgcttcca gaccgcgccg ggtgcccagc
      541 cagatcactt cgtaggagcg cgcgcgcagc agacgcgcca gggcgagagc cggaaagaca
      601 tggccgccgg tacctccggc catgatgagg atcggtcggg ccgcgatgga gctcatgaga
      661 gctcctccgc tgtggggcct gcggtccgcg cactgccgcg cgagccgccc atgacggcac
      721 cgcgctggcg gaccgtcgcc gagccgcgct tctcgagcat ggcttcgtgg taaacacgca
      781 gcagcagtcc gacccaggcg agtgagacga tgaggctgga gcggccatag ctcatcagcg
      841 gcagcgtcag ccccttggtc ggcagcgccc ccatgttgac gccgatgttg atgaaggcct
      901 ggatgccgac ccacaggccg aaagcggcgg cgagatacga ctggaatttc aggccgacct
      961 cggaagcgag ccgggcgatg tagaagctgc gccagatgag cgcgaggaaa agggccagcg
     1021 tcaggatcac gccggccagg cccagctctt ccgccagcac tgcaaacaga aagtcggtgt
     1081 gcgcttcggg cagatagaac agcttctgca cgctatcccc gagaccgacc ccgaaccact
     1141 ggccgcgacc gatcgcgatc agcgactgcg tgagctggaa gccggtgttg tacgggtccg
     1201 cccagggatt gagaaaggcg gtgagccggc gcatgcggta gctcgaggtg accgcgagca
     1261 gcccgaagcc cgcggcggcc atcgctgtca tcgcaatgac gtagcgcagc cgggcgccgg
     1321 ccagaaacag gatgccgaac ccggtggcga acagcacgct cgcagcgccg aagtccggct
     1381 cggcgagcag cagtgctgcg acgaacacca gcagcccgag cggcttgaga agacccgcga
     1441 agctgctgcg cagctcctcc tggcgccgca cggcgtagct cgcgacatac accagcacca
     1501 gcacgcgcgc gagctcggag gcctgaaagt tgatccccgc cacgtggatc cagcgccggc
     1561 ttccgttcac ggtgtggccc aggccgggca cgaagacgac cgcgagcaga acgatggcga
     1621 agatgagcag cgcgaacgag gctttctcca acagctcggt ccgcacacag aacaacagtg
     1681 cggcgcaccc tccgccgatg agcatgagca cgagctgccg cacgaggtag taaaagggct
     1741 ggccagtgtc ctggctcgca atggacaccg aggccgaggt caccatgatc aggccgagca
     1801 gcgagatcgc caggaccagg gcgaccgtca cggtgtcgat gtggaccaca ccagcgcctc
     1861 ggccggagcg cgcgtaggtc atcgaggagc ctgccaaggt gctgttcatg ccgcgagctc
     1921 ccgcaccgcc tcactgaaga ccgctccgcg ctgggtgtag tcgcggaaca tgtcgaggct
     1981 cgcacaggcg ggtgagagca acacggtgtc acccggcttc gcggcccgcg ccgcggcgcg
     2041 gactgcctcc tggagcgagg tgcaggtctc cgtcgtgcat attccctgca gagcgttctc
     2101 gatcgcacct gcatcccgcc cgatgagcac ggtgtgccga accttgccgc gaaaggccgc
     2161 cgcaagcggc gcgaagtcct gattcttgcc atcgcctccg gcaatcatga tcagcggccc
     2221 gctcattccg ccgaccgccg cgatcgtcgc cccgacgttc gtgcccttgg agtcattgat
     2281 gtaggcgaca cccctgacat ccgccaccca ctgggagcga tgcggaaggc cgggaaactc
     2341 cttgagctcc gtgagcatcg ccgccatcgg cagctccagc gcttcgccga gcgcaagagc
     2401 cgcaagggcg ttcgcggcgt tgtgcatgcc cttgatgcgc attgccgaga tcggcagcag
     2461 cgcctccgtg cgacgcatca gccagcaatc ggctccggtg atcgagatgg agtaatcggc
     2521 gccgatggcc gcgcgcagtg agaagctcag cgtgcgctgg ccttcatgcg gcattgccac
     2581 gacgagcgga tcgtccaggt tgatcacggc cgtgtcgcag cgcgcgaaga tgcgcgcctt
     2641 ggcgttcgcg tattcctcga tcgaagcgta tcgatccagg tgatccggag ttacgttgag
     2701 aaccgtagcc gccttcagcg cgagcgaata ggtcgcctcc agctggaagc tcgacagctc
     2761 cagcacatag aggtcaggac ggcgcggcgc gccctcggca ctcagcagat cgagcgcggg
     2821 ctcgcccagg ttgcccccga cacgaacgtt cactccagcg cgcgcggcca tgcggccgac
     2881 taacgtggtc acggtgctct tgccgttcgt accggtgatg ccaacgacgg gtgcatccgc
     2941 cgctcgcgcg aacagctcga tgtctccaat gatctcgaga ccccgtcgcc gcgcttccgc
     3001 gaagaacggc gcggcaagcg atacgccggg cgaggccacc acgcacaccg cgccatcgag
     3061 cagactcgtg tccaggccgc cggtgcgcag tggaatccgc ggatccagct cccgcagagc
     3121 gagcagttcc ggaggctgct cgcgggtgtc cgtcacggcg acccgccatc cgagccgccg
     3181 cagatggcgc gcgctcgaga gacccgttcg accgagaccg acgatcactg cgcaaggggc
     3241 tttggcggcg ggggtgctca tctcagcttc agcgtcgcca gcccgagcag cacgagcagt
     3301 aaggagatga tccagaaccg cacgatgacc ttcggctcgg cccacccctt gagctcgaaa
     3361 tggtgatgaa tcggggccat cttgaagatg cgcttgccgc gcagcttgaa tgagcccact
     3421 tgcaggatga ccgaagcggt ctcggcgacg aacaggccac ccatgatgag cgcgacgatc
     3481 tcctggcgca cgatgaccgc gatgacaccg atgctcgcgc cgatcgccag cgccccgaca
     3541 tcgcccatga agacctgcgc cggataggag ttgaaccaga gaaatccgag gccggccccg
     3601 gcgagcgcgc cgcagaagat gatcagctcg cccgcgccgc gcacttccgg aatctgcagg
     3661 taatgcgcga agacggcgtt gccgcttgcg taggcgaaga tcccgagcgc ggcgatcacc
     3721 agagtggccg gcatgatggc gagaccatcg agcccgtcgg tgagattcac cgcgttgctc
     3781 attccgacga tcatgaaata cgcgatcgtg atgtaggtga acgccgagag cggctcggca
     3841 aagctcttga cgaagggcag gaacagagtc gtttcggagg agctggtggc cgtgtagtac
     3901 agcgtgcccg cggtggacag gccggccacc gattgccaga agtacttcca gcgggcgatc
     3961 aggcccttgg gattgcggat gacgagcttc agatagtcgt cgtagaagcc gatcagcccg
     4021 aaggcaaacg tcacaccgag caccatccac accagccgat cggtgagctc ggcccacagc
     4081 agcgtgctga tgagcgtagt caccaggatc agcgtgccgc ccatcgtcgg cgtgccggct
     4141 ttcagcagat gggtctgcgg tccgtcatcg cgcaccacct ggccgatctg atacctcgag
     4201 agccgcgcaa tcagtggcgg acccacgagc agcgacaggg caagtgccga gaccgttgcc
     4261 agaatggcgc ggaaggtcag gtacgagaag acgttgaagc ccgaccgcca gccgaccagc
     4321 tgctgtgtca cccagtagag catctagtgg ctgccctcag ggttgtccgt cggaccgacg
     4381 agagcctcga ccacgcgctc caacctgttc atgcgcgagc ccttgatcag cattcgcacc
     4441 tccgcactcg cccctcgcag ggcctgcgtc agcgcgcccg tctcctcgaa ccactccgct
     4501 ccctctccga agctgcgggc ggcgagcgcg gcgagcgcgc cagtggcgaa cagccgctcc
     4561 acgccctgct cgcgcgcaaa gcttccgatc tccgagtgcg cgctctcggc gaattcgccc
     4621 agctccgcca tgtccccgag caccagccac ttgtgtccat cgaggcccgc cagcacttcg
     4681 atgccggcac gcaccgagct cgggttggcg ttgtaggagt catcgatcag ccaggcgcca
     4741 tcacgagtca tcttgaactg cagtcgcccg gggaccgagc gcactgcgcc cagaccgctg
     4801 acgacgtgac tcagatgcgc gccggcggcc atggcggctg ccgccgccgc gagggcgttc
     4861 gctacgttat gccgcccgcc catcttcaac tcgacgccgg cagcgccggt gggtgtcagc
     4921 agtgtgaagc gcgtgatgaa gccttcggag cccacggtgg tctgcacctc gatggcactg
     4981 aagtccgccg gctttctcat cccgaaggtc gtgatccggg ccggggtcag cgcgcgccac
     5041 agatcggcga actcatcatc agcattgatc accgcagtcg cactgctatc gaggccggca
     5101 accatttcgc cctcggcgcg tgcgacccct tccaggctgc caaagccttc gaggtgctcc
     5161 accccggcat tggtgatcag gcccaccgtc ggccgtgcga tccggaccag ggcggccacc
     5221 tcgccggcgc gattggcgcc catctcgatg acggcgaagc ggtgatgagg ctcgatcctg
     5281 agcgtcgtga gcggtacgcc gatgtggttg ttgagattgc cgcgcgtcgc gagacagtcg
     5341 cccgcctcag cgaggatggc cgcggtcatt tccttggccg tcgtcttccc gttgctgcct
     5401 gcgacgccca cgatcgggat ggtgtactgc gcgcgccaga cgctggccgc gcgctccagt
     5461 gccgactgag tgtccgccac gagaatctgc ggaagggcga ccggctggcg cgtatcgacc
     5521 agcgcgccgg ccgctccggc cgccagagcc gccccgacga actcgttgcc gttatgctgt
     5581 ggtccgtgca gagcgatgaa cagatctccg cgcgagagcg tgcggctgtc gctccagaca
     5641 gcctcatagg ggccgtcctc accttcgagc gtgccgtcac aggcccgggc aaaatcgctc
     5701 aacgagcgca tcatggccgc gctcccgcga gctccgactc gacgatggcc tggtcgctga
     5761 acgctcggcg cacgtggccg tgaatctgat agtcctcgtg ccccttgccg gcgatcagca
     5821 ccacatcggc ggcagcggag cgtcgcagcg caagttggat tgcccgggcc cgatcgtgct
     5881 cgacgagagc cgccgtccga tccggcatgc cgttcaggat gtcggcaacg atgcgaccgg
     5941 gatcttcggt gcggggatta tcgtcggtga cgatcacttc gtcggccagc tccgtggcga
     6001 tgcgccccat caccgggcgc ttaccgacat cgcgatcacc gccacaaccg aacaccaccc
     6061 gcagccgacc ccgacagtgc agtcgcgcgc cctgcagagc cttcgcaagc gcatcaggcg
     6121 tgtgcgcgta atccacgatg gcaaggggcg ccgtaccctg tccgccaaac agctccatgc
     6181 ggccgctagg ggcccggcac tgcgcgagcg cgagtcgggc ctgcgccagc gggatgttcc
     6241 aggcgagcag cactgccagg acggtgagca cgttgtcgac gttgaactcc cccaccaggg
     6301 gcactttgag ctcgacttcg ccccagctcg agtccacacc gatcgtcatg ccggcggcct
     6361 ccggctgcgt gcgggtggcg cgcacgtacc cggcaagcgc cggcacgctg tcgtgtgccg
     6421 cagccgcgcg gctcgtgacg atgaggcggg ctcgggaggt ctgatgggcg aggtttgcgc
     6481 cgaaggggtc atcgatgttg atgatccgga tggcgagcga gggccactcg aacaggcgcg
     6541 ccttggcagc cccataggcc tgcatcgtcc cgtgataatc cagatgatca cgcgtcaaat
     6601 tcgtaaaggc agcggtgttg aagcgcacgc caccgacccg atcctggtcg attgcatgcg
     6661 aggacacctc catgcacacg cattccgcgc cgagctcctt gagcgccgcc aactggcggt
     6721 gaacgctgac ggcatcaggc gtggtgtgct gcgtcggggt gacctgcgga ggcacgccgt
     6781 agccgagcgt acccatgtag gcggcaggac ggccgctgtg ttgcagggcc tgagccagaa
     6841 gccaggcgca ggttgtcttg ccgttggtgc ccgtgatccc cgcaatcgtg agcgccgcgg
     6901 agggcgcggc aaagaaccga tcggcgatct caccgacgtg gcggctcagt tgcggaaccg
     6961 cagcgacgaa gatcgccttc ccgagctgct cggcaaggag ttcgatgcgc cgcgccggct
     7021 caccgggcgc ggcgctctcg tacagcacgg cgctcgcgcc ccgtgctacc gcctccggcg
     7081 caaactcgag cccatgagag gcgcgcccct gacaagccag aaacaacgac ccggagctcg
     7141 ccgcccggct gtcgagcgtg acatcactga tgcgaagatc cgaaggcacg acagcgagcc
     7201 cggcggtcag gtcagccagc ttgtgcgccg ctgcactcat cgcagtgccg ccgtacgcac
     7261 ttgcgtgtcc gtgggactcg ggggcaactc atcgggcgca ttcacaggac gatcgggcgc
     7321 caccgccagc aggcgcaacg cgccgcccac cacggcagaa aacaccggcg ccgagacctg
     7381 tccaccctgg tgcagtcccg cgctcggctc atcgatgacg accacggccg caagccgcgg
     7441 ggcactggcc ggtgctacgc caccgaacac cgccatgtat cgatcggtcg agtagccgcc
     7501 cgagatgatt ttgtaagcgg tcccggtctt gcccgagaca cgatagccgg gaattgccgc
     7561 gcgcttgccc gtcgcgccct ctgcgatcac taccgactcc agcatgccca ccagctcacg
     7621 ggcgccgtgc tcatccatca cgcgctgtcc ggctggaggg gtatccacgc gcacaaagga
     7681 gatcggtttg ctgataccga agctgccgac cgtggcgtac gcatgtgcga gctgcagcgg
     7741 cgtcacggag atgccgtaac cgtgggacat cgtggcaatt ccgaccggac gccactggga
     7801 gtagttggcc agcagtccgg cggactcacc cgggtagccg ctcgtggtca cctgcccgaa
     7861 gccgaatcgg gtgagcgtgc tccagatcaa cttgggctcg aggctgagcg caagccgcgc
     7921 catcccgacg ttggagctct tggccagcac ggtggcaatg ttgaccgcgc cgaggttgtg
     7981 ctcgtcctcg aagatcttca cgccgacctt gtagaagccc ggcgaggtat cgatgatgct
     8041 gtgatcgtcg tacttgcccg actcgatggc ggcggccacg aagaaaggct tcatggagga
     8101 gccgggctcg agaatgtcgg tcgcggcccg attgcggtac aggtccggca ggatctgatc
     8161 gcggtcgttc gggttgtagg ccggttggtt caccatcgcc agcacttcac cggtggtgac
     8221 atcgagcacc acgatcgagc cggccttcgc gcgttgctcg cgaatggccg acttcagttc
     8281 ccgataggcg aggtactgga tgcgcagatc gagcgacagc acgagatcgc gccccggccg
     8341 cgccgggcgg atgctctcga cgtcctgcac gatgcggccg tagcggtcct ggatgacacg
     8401 cttggcgccg ttctcgccgg caagccagtg atcgaacgcc agctcgagcc cttcctggcc
     8461 ggcatcatcg acattcgtga agcccagaat gtgaccggcc acttcgccgg ccgggtagta
     8521 gcggcgatat tcgcgcgaca ggtacacgcc ggggatgccg agtgccttga tctgctcggc
     8581 ctcactcggc tgacggccgc gggcgagata caggaactcg cggtcgagat tgctcgtgat
     8641 gcggcgcgtg agctcctggc ggtcctgctt caacgcgctg gcgaggcgcg gaatctgatc
     8701 cgtggcgagc gcgagctcct tcggattgac ccagatcgaa tccaccggag tactcacggc
     8761 cagcggctca ccgtaccgat cggtgatcgt gccgcggtgg gcggcaatct cggcgacgcg
     8821 cgagaagcgg gcatcgcctt ccttcgccag gaagctgtga tcgacgagct gcagattgac
     8881 ggcacgccac agcagcccac aggcacagca gcccagcagg ccgatcagca ggtaggccct
     8941 ccagcggaaa gagagtggcg agccctcgcg agtgttacgg gccttcatgg cgcgacgatc
     9001 tcgatctctt ttggaggcgg catggccatg cgcagtttcg tgctggcgac ccgctccacg
     9061 aaggcgtgcg tggaccaggc actttgctcg agctgcagct gcccccactc gatctcgaga
     9121 ttgtcgcggc gcgcgctcag gcgctcgagc tcgacgaaca gctcacgagc gcgatgcttg
     9181 cagtaaacgg ctccggctgc agagccgagc gctgcgagcc acagaagcgg caacgcaacc
     9241 agaaaaatcc ggcgccagag cgagctcatg tgagcttctc ggcgatgcgc agcagggcgc
     9301 tgcgagagcg gggattgcgt tggatctcct gctctgcggg ccgcgtcttg cgaccgatga
     9361 gcctcagccg gggaggagcc acggaaggcg gcaggatagg cagcgctgcg agttgcggat
     9421 cgagctggga ttcgcgctgc aggaagcgct tcacgagccg atcttcgagg gagtggaagc
     9481 tgatcacggc gaggcggccg ccgggggcga gcacctcgag ggctgcggcg agccccgcct
     9541 cgagctggcc gagctcatcg ttgatgaaca tgcgcagggc ctggaaggtt cgcgtcgccg
     9601 gattcttgcc gggctcgcgg gtacgcaccg ccttggcgac cagggcggcg agctgagccg
     9661 tgcgcgtgag gggacgttgt tcccgctcgg cgacgatggc gcgcgccaca cgtgcagcga
     9721 agcgctcttc tcccagcgtt gcgatcactt cgcgaatctc gtcgaggcgc gcccgagcga
     9781 gccacgctga gaccggctcg ccacgtgtcg ggtccatgcg catgtcgagc ggaccgtcgg
     9841 cgcggaaact gaagccccgc tcggggtctt cgagctgggg cgatgaaaca ccgaggtcga
     9901 agagcacgcc gtggcaaggt tgaccgcgcg agtgagcggg cacgagcgcg gcgagatcgg
     9961 caaacgacgc atgcaggagc gtaagccgca cttcgtcggc aaaacgtcgg gcgccggccg
    10021 cgatggcttg cggatcccgg tcgatcgcga gcaggcgccc ctggctgccg aggccctcga
    10081 ggatgtgtgc tgtatgaccg ccccgcccaa acgtcgcatc gacatagtaa ccgcccgctt
    10141 cgagggccaa agccgcgagc gcctcatcga caagcacagg tgagtgctca tccacgatcg
    10201 aagcggtgat ggacccgccc cccgaagagt tgccgcgttt tcgccccacg cgcgcccgcc
    10261 aaggcgcgcc gcccgctcaa tgcccagcac accccgcgtg cactgcgccc ccaaagcccc
    10321 tgtcccagcc gccccgatct gcgaaccccg tgctcatagc gacagcgaat cgagctcgga
    10381 gggcagatcg gtcgccgcct cctcgctctt cagccactga tcgcgccgct cgccccagcg
    10441 tgtctcgtcc cacagctcga agcggttccc ctggccaatc agcatcccgt gccgctcgag
    10501 ttgcgcgaac tcgcgcagct cgggcggcag gagcacacgg ccgtgcccgt cgatctcgac
    10561 atc
//
LOCUS       DASRHN010000130        13813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1361129, whole genome shotgun sequence.
ACCESSION   DASRHN010000130 DASRHN010000000
VERSION     DASRHN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361129"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VFB37_04990"
     CDS             <1..1335
                     /locus_tag="VFB37_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO21840.1"
                     /translation="SLSSFIGVDGNNVTLNSGRFLINLKPRDSRTLTASDIIRRLREE
                     TADVPGITLYMQPVQDLTLDNTISRTQYQFTLESADALALATWVPKLVSVLRQQPDLS
                     DVVSNQEDDGLAVYITVDRDSAARLGISVGTVDNALYDAFGQRIVSTIFTQSNQYRVI
                     LEADPGAYGSVDSLASLYVPSASGGQVPLSAIAKVSVQSRPLLINHLAQFPATTVSFN
                     LAAGASLGAAVNAIEKSERDLQLPASIRTTFQGAALAFRSNLTNELLLLIAAVLVMYI
                     VLGVLYESFIHPITILSTLPSAGIGALLALIIAGDDLTIIALIGIILLIGIVKKNAIL
                     MIDFAIDAEREHGLTPREAIHQACLLRFRPILMTTVAAIFGALPLMFGTGTGSELRHP
                     LGISIVGGLLVSQVLTLFTTPVIYLAFDRVAKRLMRRGDGDQRDPDLADNPA"
     gene            1332..4775
                     /locus_tag="VFB37_04995"
     CDS             1332..4775
                     /locus_tag="VFB37_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO21841.1"
                     /translation="MNISAPFIARPVATILLTFGVALCGMVAFTLLPVAPLPKIDVPA
                     IFVSASLPGASPEVMASSVATPLERHLGAISDVDDMTSTSGVGATVIQLTFGLDRDID
                     GAARDVEAAIVGARADLPSALTRNPTYRKLNSSLFPVLSIAMTSDVLTQGQIYDAADA
                     VISQRLSQINGVGGVSVSGSALPAVRVEINPLAVSKYGIGLQDIRAAISNANANAPKG
                     AIETDKLHYQIYTNDNARDAEPYRDLIIANRNGNMVRLGDVAAVTDMQDGATEDIRTY
                     GVYNGKAAVSVQVYQQAGANIIQVVDAVKAELPILKASIDPKIDLAITYDRSITIRAS
                     LHQVEQTLLIAVAMVILVVYMFLHSYRAALVPAIVVPISLIGTFGAMYLLGYTLDNFS
                     LMALTIATGFVVDDAIVVMENTARHIEDGMTPMRAALQGAREVGFTVVSMSLSLIAVF
                     IPFQLIGGLVGRLFREFTVTLSIAILISLALSLTTTPMMCSRLLRRERKRNFSRLGRS
                     FEHLFESSREGYARTLGWALDHPRMTMLLLLATVCLNIYLYIAVPKGFFPQQDTGQLR
                     GGIRGDADASFQLMKTKLQQVARIVQADPAVEKVTGTVGSGGFRPSSGASASINVTLK
                     PLAERKISADAVIARLRPKLAHVRGVATFLQAVQDIGGGGGRSANSEYQYTLMGDDLA
                     ELRTWAEKLRLALQDVPEVQDVDSDLQPGGLEADLIVDRDSASRLGLTESQIDNSLGD
                     AFAQAQVSTIYNPFSPQQYHVVMEVAPEYWQNPEVLKELYVSTSGGAVTGTQATQAVA
                     GTTLLKARTASTSGASTASTGSSAAASPLASTTGAAATGSASATSASASTTATGSLSA
                     TAAAVAQDAARNLAANSLANAGRGNTSTGSAVSVAAETVVPFSAFSHFATGTTAVSVN
                     HTGTSVSTSISFNLPEGEPLGVALAAIERTMNQIHMPISIHGGSYGNARLFQQNASAQ
                     PLMLAASLLAIYVVLGMLYESYTQPITILSTLPSAGVGALLALMATDTEFSLIAFLGI
                     LLLIGIVKKNAIMMVDFALEAERTLGLEPRAAIARACHLRFRPIMMTTFAAIAGALPL
                     AIASGDGTEMRRPLGISIVGGLVVSQLLTLYTTPVVYLYIERWSRRKRAQTRLQWQPG
                     T"
     gene            4799..6169
                     /locus_tag="VFB37_05000"
     CDS             4799..6169
                     /locus_tag="VFB37_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO21842.1"
                     /translation="MGAVFALLSACAVGPSYHRPAFNTTPTYKEESGWKPSEPADALD
                     RGPWWLIFHDDFLSSLEAQVNISNENVLAAEAAFEQSRALLKQAQASLWPSVSIDATR
                     GRTVTGDGAPHTLNSLGASASWDIDLWGQIRRGVESSRASAQANAASLAAARLSAQAQ
                     LATAYFQLRAQDQQQIILDDIVAAEQLSLKITENRYRVGVAAKADVVTAQTQLLSSQA
                     QQVSAPLQRALLEHAIAVLIGQQPASFSAPVAPIRTDVPTVPAGVPSTLLERRPDVAE
                     AERKVAAANAQIGVAVAAFFPNLTLSGSDDYTGNAISRLIRTSNRVWQFGPALALSVF
                     DAGLRRAEVAQARAAYDASVDSYRQTVLAGIEQVEDEIATLRILEQQSVIEDAAVKAA
                     REAEKLTLNQYKAGTVPYSSVITAQTTRLGSEETALGVLSNRLQASVALIEALGGGWK
                     TSDLPN"
     gene            6179..7618
                     /locus_tag="VFB37_05005"
     CDS             6179..7618
                     /locus_tag="VFB37_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21843.1"
                     /translation="MSAPEPPRAVAEVDDRSGSLASASGPEADGLPVPRRYWAILAIV
                     LAISMSVLDSSIANVALPSIARHFHATSAASIWVINAYQIAILMALLPLASLGEVVGY
                     RRISQAGLGVFTVASLACAFAPSLLTLSLARVIQGIGAAGIMSVNAALVRFTYPHRML
                     GRAIGINAFVVATCAAIGPTIASAILAVGSWRWLFGINVPIGLITILIATRALPETER
                     LKRPLNYLGAALSAGTFGVLIGGLQALAHDGAAPLALAQIVAGCVLGLILVRHERRRA
                     APLLPFDLLKIRLFTLSLATSVCSFMAQTSALVALPFEIQRLGHTPVETGLLMTPWPL
                     ALAVAAPVAGRLADRYPAAVLGGIGLLVMGSGLMLLACFPESGSPAGFVWRMVLCGLG
                     FGFFQSPNNRAMLSSAPRARSGAAGGMLSTARLLGQTLGAAGVAILFRTYDARGSNFA
                     LFAAAALAIAAAIVSLLRLPAVGSTTARL"
     gene            complement(7625..9727)
                     /locus_tag="VFB37_05010"
     CDS             complement(7625..9727)
                     /locus_tag="VFB37_05010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZO21844.1"
                     /translation="MADLPSDNPFARPSTLPFQLPPFDRIHDTDYLPAFEAGMRAQRD
                     EVTQIAQARNAPTFENTLVALERSGELLERVSSAFSNLNASNTNPQMQRIESEIAPRL
                     QSHEDAIFLDSALYARVDAVYRTRSGLQLDAESAQLLERYHTEFVRAGAQLSETDKQH
                     LRSLNEQLSGLTTRFKQNVLKATQDGAVLVDRVAELDGLSAEQAGAAALAAQARGMPG
                     KWLIPLQNTTNQPLLAQLTNRGLRERIYKASAGRGLGVSSGSGPGADNRGVIAQIVRL
                     RAERAALLGYPNHAAYVLADESAASSAAVHQILGQLVPAALARAKDDARQLQMIIDAE
                     AAVRHTATFALQPWDWEFYAEKLRASRYDFDQSQVKPYFELDHVLQDGVFHAAHELYG
                     LTFKERKDLPVYQPDVRVFEVLDADGSPLALFLGDYFARDNKQGGAWMNSFVRQSKLL
                     GRKPVVGNNLNIPKPQAGQPALLTFEEVTGLFHEFGHAVHAMLSSVQYPLLSGTSVPR
                     DFVEYPSQYNEMWAREPAVVAHFARDYRSGAPMPEALLEKVLAAQKFNQGYALTEYLA
                     AALLDQSWHQINPAQAPAAAEVAAFETRALHEVGIDYPPIATRYHSSYFSHIFAGGYS
                     AAYYAYLWSEVLARDTGKWLHEHGGLTRANGDILRAKVLSRGRSSEPQVLFRDFYGGP
                     PDIGPLLQYRGLSAPAAP"
     gene            9969..10454
                     /locus_tag="VFB37_05015"
     CDS             9969..10454
                     /locus_tag="VFB37_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO21845.1"
                     /translation="MLSIKQDSVVTFHYTLRDDAGEIIDRSDAGQPLSYLQGHGNLVP
                     GLEQALEGKSAGEKLSVKVSPAQGYGEYSQDLLQQVPRRSLKGIGRLTPGMRLHAQTA
                     QGPRTVTVTRITGDMVALDANHPLAGKNLNFDIEITGVREATQEELAHGHVHGPGGHH
                     H"
     gene            10464..10949
                     /locus_tag="VFB37_05020"
     CDS             10464..10949
                     /locus_tag="VFB37_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO21846.1"
                     /translation="MSRPRALYAASLALALLSWTGASLASRCPTAPESEKKAILELIN
                     RMQDAWNRGDFRGYMQGFKNPDVIFVSGGRFQSGWQGTLDHYVRDYGASAETRGRLRF
                     FNIRIEMLAPDAAQLISQFELLRPQHPQYGINTRLMRKVDGRWVIALNHVSASETPPQ
                     R"
     gene            complement(11000..12052)
                     /locus_tag="VFB37_05025"
     CDS             complement(11000..12052)
                     /locus_tag="VFB37_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZO21847.1"
                     /translation="MSTPDVSVAQKIARYALIGSLVALGAWMLQHFLAALGWAVVLAI
                     ATSSLYDRWLAKFRGKHRHIWAALSFTAIVGIVLIVPLVYGGVVAVREAISLVRAYLE
                     SSKGGPPELPQWVVQLPLVGGWIHDMWLEQVHHAGAAAAPAKPAVYEWTRMVGVQLLR
                     RLSTLVFTLLTLYFVYLNRDGLRSDVPRVSRRLFGPTVESLLTRAAEAVRATVDGIVL
                     VAVAEGAIMAGVYAVTGVRHPILIGVVTGIFAMVPFAAPVAFGIVALLLFSQGAVGPA
                     IGVLVCGFVMLFIADHFVRPLIIGEGAKLPFLWVLLGILGGIESFGLIGIFLGPALMA
                     ALVSLWRGWVAHSRPP"
     gene            12107..13369
                     /locus_tag="VFB37_05030"
     CDS             12107..13369
                     /locus_tag="VFB37_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HZO21848.1"
                     /translation="MAPTAEPLSRAPSPSPGPAAERIGCPDCGLLQRLPLPGRGQLIE
                     CARCGRTLAGPATGQVSTPFALTVSALLLLLPALIEPLMTVSSYGAHRSIALPASAEA
                     LWHDGFPSLGLLVGAFSIALPLVFLSLLIWVFARLQMGSTQGVGTAFRWTRHLRPWVM
                     IEVYLVGGLVAYSRINMMTSVEIGSGGWCLVASSLVLLVALIGLDERTVWERLARPVP
                     PATGSTLACSVCDLIVAPDATRCPRCHASLAVRKPHSLQWTAALVAAGYLLYVPANLL
                     PVLTTVRLGREEHNTILSGIIELARNDLVPLAVIVFAASIVLPIVKLFGLTWMLLAIR
                     SRSGLYLVGRTRLYRVIDAIGRWSNIDVFAVAVLVALLQFGELTAVHAGNGLVAFAAV
                     VVITMLATMVFDARLMWDAVRSRHERRP"
     gene            13353..>13813
                     /locus_tag="VFB37_05035"
     CDS             13353..>13813
                     /locus_tag="VFB37_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO21849.1"
                     /translation="MNEGPEEREQEEEPRARLRPHGWFGWAWLIPIGAAAVLLWLAWR
                     GLAERGPGITISFRNAEGLQAGQTKIQHRNVDVGTVESLELTPDMAHVIVHARMTRVA
                     SDHLTANTRFAIIAPHVGVGGISGLSTIVSGSYIEMYPGTPGPPQRQFVGL"
BASE COUNT         2354 a         4669 c         4366 g         2424 t
ORIGIN      
        1 agcctgtcct cgttcatcgg cgtggacggc aacaacgtca cgctcaatag cgggcgtttc
       61 ctcatcaacc tgaagccgcg cgactcgcgc accctcaccg cgagcgacat catccggcgg
      121 ctgcgagagg agacggcgga cgtcccgggg atcactttgt acatgcagcc ggtacaggat
      181 ctcacgctcg acaacacgat cagccgaact cagtaccagt tcacgctcga gagcgccgat
      241 gcactcgccc tggcgacctg ggtgccgaag ctcgtgtcgg tgctgcggca gcagccggat
      301 ctctccgatg tcgtgagcaa tcaggaggac gacgggctgg ccgtgtacat cacggtcgac
      361 cgcgacagcg cggctcgact cgggatcagc gtgggcacgg tggacaacgc cctctacgat
      421 gccttcggac agcggatcgt ctcgaccatc ttcactcagt ccaatcagta tcgcgtgatc
      481 ctcgaagcgg acccgggcgc atacggctcg gtggattcgc ttgcctcgct gtacgtgcct
      541 tcggcgagcg gcgggcaggt gccgctctcg gccatcgcca aggtcagcgt gcaatcgcgt
      601 ccgctgctca tcaatcacct ggcccagttc ccggccacga cggtgtcgtt caacctggcg
      661 gccggggcct cactcggcgc cgcggtcaac gccatcgaga agagcgagcg cgacctgcag
      721 ctgccggcga gcatacgtac gaccttccag ggcgctgcgc tcgccttccg cagcaacctg
      781 accaacgagc tgttgctgct gatcgccgcc gtactcgtga tgtacatcgt gctgggcgtg
      841 ctgtacgaga gcttcattca tccgatcacg attctctcga cgctgccctc ggccggtatc
      901 ggcgctctcc tcgccctgat catcgccggc gacgatctga cgatcatcgc cctgatcggc
      961 atcatcctgc tcatcggcat cgtcaagaag aacgcgattc tcatgatcga cttcgccatc
     1021 gacgccgagc gcgagcatgg cctcacgccg cgcgaggcga tccatcaggc ctgtctgctg
     1081 cgcttccgtc ccatcctcat gaccacggta gcggcgatct tcggcgcttt gcccctgatg
     1141 ttcggtaccg gcacgggctc cgagctgcgt catccccttg gaatcagcat cgtcggaggg
     1201 ttgctggtga gtcaggtgct cactctcttc accacgccgg tgatctacct tgccttcgac
     1261 cgtgtcgcca agcgcctgat gcgccgcggc gacggagatc agcgcgaccc ggatctggcg
     1321 gacaacccgg cgtgaacatc tccgctccct tcatcgcccg gcccgtcgcc accatcctgc
     1381 tcactttcgg cgtggctttg tgcggcatgg tggcgttcac ccttctgccg gttgcgccgc
     1441 tccccaagat cgacgtaccc gccatcttcg tctcggcctc tctgccgggc gccagtccgg
     1501 aagtcatggc gagctcggtg gcgacgcccc tggagcgaca cctcggcgcg atctccgatg
     1561 tggatgacat gacatccacg agcggcgtcg gcgcgacggt cattcagctg accttcgggc
     1621 tcgatcgcga catcgacgga gctgcgcggg atgtggaagc ggcaatcgtc ggagcccgcg
     1681 ccgacctgcc cagcgccctg acacgcaatc cgacctaccg caaactcaac agctcgctct
     1741 tcccggtgct gtcgatcgcg atgacctccg atgtgctcac acaaggtcag atctatgacg
     1801 ccgccgacgc ggtgatctcg cagcggctgt cgcagatcaa cggtgtcggc ggagtgagtg
     1861 tgagcggctc ggcactgcca gcggtgcgcg tcgagatcaa tccactggcg gtctcgaagt
     1921 atggcatcgg gctgcaggac atccgcgcag ccatctccaa cgccaatgcc aatgcgccga
     1981 aaggagcgat cgagaccgac aagctccatt accagatcta taccaacgac aacgcccggg
     2041 atgccgagcc gtaccgggat ctcatcatcg cgaaccgcaa tggcaacatg gtgcggctcg
     2101 gcgatgtggc ggccgtcacc gacatgcagg acggggcgac cgaggacatc cgcacctacg
     2161 gcgtgtacaa cggcaaggcg gctgtttcgg tacaggtcta ccagcaggcc ggtgccaaca
     2221 tcattcaggt ggtggatgcc gtcaaggcgg agctgccaat cctgaaggct tcgatcgatc
     2281 cgaagatcga cctcgccatc acttacgacc gctcgatcac catccgggcc tccctgcacc
     2341 aggtggagca gacgctgctg atcgccgtgg ccatggtgat tctggtcgtc tacatgttcc
     2401 tgcacagcta ccgcgccgcg ctggtgccgg cgatcgtcgt gccgatctcg ctcattggca
     2461 cattcggggc gatgtacctg ctcggctaca ccctcgacaa cttctccctc atggcactca
     2521 cgatcgccac cggtttcgtc gtcgacgatg ccatcgtcgt catggagaac accgcgcgcc
     2581 acatcgaaga cggcatgacc ccgatgcggg ccgccctgca gggcgcgcgt gaagtgggct
     2641 tcaccgtcgt gtccatgagc ctgtcgctga tcgccgtgtt cattcccttc cagctcatcg
     2701 gcggcctggt cggcagactg ttccgcgagt tcaccgtgac gctgtccatc gcgatcctga
     2761 tctcgctggc gctgtcgctg accacgaccc ccatgatgtg ctcccgtctg ctgcggcgcg
     2821 agcgcaagcg caatttcagc cggctcggcc gcagcttcga gcatcttttc gaaagctcgc
     2881 gggaaggtta cgcccgcaca ctcggatggg ccctggatca tccgcgcatg accatgctgc
     2941 ttctgctggc aactgtttgt ctgaacatct atctctacat cgcagtgccc aaaggcttct
     3001 tcccgcagca ggacaccgga cagctgcggg gcggtatccg cggtgacgcc gatgcgtcct
     3061 tccagctgat gaagaccaag ctgcagcaag tcgcccggat cgtgcaggcg gatcctgcgg
     3121 tggagaaggt gaccggcacg gtgggcagcg gcggctttcg tccttccagc ggcgccagtg
     3181 ccagcatcaa cgtgactctc aagccgctgg ccgagcgcaa aatctccgcc gatgcggtga
     3241 tcgcccgttt gcgcccgaag ctcgcccacg tccgcggggt ggcgacattc ctgcaggccg
     3301 tgcaggacat cggcggcggc ggcggccgct ccgccaactc cgaatatcaa tacacgctga
     3361 tgggagacga tctggcggag ttgcgcacct gggcggagaa gctgcgcctc gcgctgcagg
     3421 atgtacccga ggtgcaggac gtcgattccg acctgcagcc gggcggcctg gaagcggatc
     3481 tgatcgtcga ccgcgacagt gcctcgcgtc tggggctcac cgaaagccag atcgacaact
     3541 cactcggcga tgccttcgcg caggctcagg tctccacgat ctacaatcct ttcagtccgc
     3601 agcaatacca cgtcgtgatg gaggtggcgc cggaatactg gcagaacccg gaggtcctga
     3661 aggagctgta cgtgagcacc tccggtggcg cagtcacggg cacgcaggcc acccaggcgg
     3721 tcgccggtac gacgctgctc aaggcgcgga ctgcctcgac gtcgggcgcg agcaccgcct
     3781 cgacaggctc gtctgccgcc gcgagccccc tggcgagcac aaccggtgca gccgccaccg
     3841 gctccgccag cgcaacttct gcgagcgcct cgacgaccgc aaccggatcg ctcagcgcca
     3901 cggcggccgc agtggcccag gatgccgcgc gtaaccttgc ggccaactcg ctggccaacg
     3961 ccggccgcgg caacacctcg accggcagcg cagtcagcgt ggccgccgag acggtggtgc
     4021 ccttctcggc cttcagccac ttcgccaccg gaacgaccgc cgtcagcgtc aatcacaccg
     4081 gcacctcggt gtccacttcc atctccttca acctgcccga aggcgagccg ctgggtgtcg
     4141 ccctggctgc catcgagcgg accatgaacc agatccacat gccgatcagc attcacgggg
     4201 gctcatatgg caacgcgcgt ctcttccagc agaacgccag tgctcaaccg ctgatgctgg
     4261 ccgcctcact gctggcaatc tacgtcgtgc tgggcatgct gtacgagagt tacacccagc
     4321 cgatcacgat actttcgacg ctgccctcgg ccggcgtcgg ggctctcctg gcgctgatgg
     4381 ctaccgatac cgaattcagc ctgatcgcct tcctcggcat cctgcttctg atcggcatcg
     4441 tcaagaagaa tgccatcatg atggtcgatt tcgctctcga ggcggagcgc acgctgggcc
     4501 tcgaaccgcg cgccgcgatc gctcgcgcct gccatctgcg cttccggccc atcatgatga
     4561 ccacgttcgc tgcgatagcg ggcgcactgc cgctggcaat cgcctcgggc gacggcacgg
     4621 agatgcgccg cccactgggc atctccatcg tgggaggact ggtcgtcagc cagctcctca
     4681 ctctctacac gacgccggtc gtctacctct acatcgagcg ctggagccgc agaaagcgcg
     4741 cgcagacccg cttgcaatgg caaccgggca catgatgtcc ggcgttcggt gtgtgtgtat
     4801 gggggcggtg ttcgcgctcc tgagcgcctg cgcggtgggg cccagctacc atcggccggc
     4861 gttcaacacc acccccactt acaaggaaga gagcggttgg aagccgagcg agccggccga
     4921 tgcgctcgat cgcggcccct ggtggttgat tttccacgac gacttcctga gctcgctcga
     4981 agcgcaagtc aacatctcga acgaaaacgt gctggcagcc gaggcggctt tcgagcagtc
     5041 gcgcgccctt ctcaagcagg cgcaggcgag cttatggccg tcagtgtcca tcgatgcgac
     5101 gcgcgggcgg accgtgacgg gcgatggagc gcctcatacg ctgaacagcc tcggcgcgag
     5161 tgccagctgg gacatcgacc tctgggggca gatccgccgc ggcgtggaga gcagccgggc
     5221 ctccgcacag gcgaacgcag cgagtctggc tgccgcacgc ctctcggcgc aggcgcagct
     5281 cgccacggcc tacttccagc ttcgcgccca ggaccagcag caaatcatcc tcgacgatat
     5341 cgtcgcggcc gaacagctct cgctgaagat cactgagaac cgctaccgcg tcggcgtcgc
     5401 cgccaaggcc gacgtggtaa ccgcgcaaac ccagcttctg tccagccaag cgcagcaggt
     5461 cagtgcgcct ctgcagcgag ccctgctcga gcacgccatt gccgtgctga tcggccagca
     5521 gccggcgagc ttttcggcgc ccgtcgcacc gatcaggacg gacgtgccca cggtgcccgc
     5581 aggcgtcccc tccactcttc tcgagcgccg acccgatgta gccgaagcgg agcgcaaggt
     5641 ggcggcagcc aatgcgcaga tcggggtcgc cgtggccgcg ttcttcccca atctcacctt
     5701 atccggctcg gacgattaca ctggcaacgc gatcagccgg ctgatcagga cttccaaccg
     5761 cgtatggcaa ttcggacccg cactcgcgct gtcggtattc gatgccgggc tgcggcgcgc
     5821 cgaggttgca caggcacggg cagcttacga tgccagtgtc gacagctatc gccagacggt
     5881 gctggcaggc atcgagcagg tggaggacga gatcgctacg ctgcgtatcc tcgaacagca
     5941 atcggtcatc gaagatgccg ccgtgaaggc cgcgcgagaa gccgaaaaac tgacactcaa
     6001 ccagtacaag gccggcaccg tcccctacag cagtgtcatc accgctcaga ccacgcggct
     6061 cggcagcgag gagaccgcgt tgggcgtgct ctcgaaccgg ctgcaggcca gtgtggctct
     6121 gatcgaggct ctcggcggcg gctggaaaac ctcggacctc cccaattgac cccgccccat
     6181 gtccgctcct gagccgcctc gcgcggtggc ggaggtggat gatcggtccg gttctctggc
     6241 aagcgcctcg gggcctgagg ccgatggctt gcccgtgcca cgccggtact gggcaattct
     6301 cgcgatcgtt ctcgcgatca gcatgtccgt gctggacagc agcattgcca atgtcgcctt
     6361 gccgtccatc gcccggcact ttcatgcgac gagcgccgca tcgatctggg tcatcaacgc
     6421 ctaccagatc gcaatcctca tggcgctgct gcctctcgcc tcgctcggtg aggtcgtcgg
     6481 ctatcgcaga atctcgcaag ccggtctggg ggtattcacc gttgcttccc tggcgtgcgc
     6541 ctttgcaccc tcgctgctca cgctcagcct cgcaagggtg atccagggta taggagccgc
     6601 cggcatcatg agcgtgaatg ccgcgctggt gcgcttcacc tatccgcatc gcatgctcgg
     6661 ccgtgcgatc gggatcaacg ctttcgtcgt ggcgacttgc gccgccatcg gccccacgat
     6721 cgcctccgcg attctcgcgg taggctcctg gcgctggctc ttcggcatca acgtgccgat
     6781 cggactgatc accatcctga tcgcgacccg ggccctgcct gaaaccgagc ggctgaagcg
     6841 tccgctgaac tacctggggg ctgcgctgag cgcgggcacg ttcggagtgc tgatcggagg
     6901 acttcaggcg ctcgcccacg atggcgcggc accgctcgcg ctggcgcaga tcgtcgcagg
     6961 gtgtgtgttg ggactcatcc tcgtgcggca cgaaaggcgc cgcgcggcgc cgctgttgcc
     7021 cttcgacctg ctgaagatcc gccttttcac cctctcgctc gcgacgtctg tctgttcctt
     7081 catggcccag acttcggccc tggtcgctct gcccttcgag atccagaggc tcggacatac
     7141 cccggtcgag accggcctgc tcatgacccc ctggccgctg gccctggcag ttgccgcccc
     7201 ggtggccggg cggctggcgg atcgctaccc ggccgcagtg ctcggtggga tcggcctgct
     7261 cgtgatgggc agcgggctga tgctgctggc gtgctttccc gagagcggct cgcccgcagg
     7321 cttcgtatgg cgcatggtgc tgtgtgggct cggcttcgga ttctttcagt cgcccaacaa
     7381 ccgggccatg ctctccagcg cgccgcgtgc gcgcagcggc gcagccggcg ggatgttgag
     7441 tactgccagg ctgttgggtc agaccctggg tgccgctggt gtcgccatcc tgtttcggac
     7501 ctacgatgcg cgaggctcca atttcgcgct gtttgcggct gcggcactcg cgatcgctgc
     7561 tgctatcgtc agcctgctgc ggctgccggc tgtcggcagc acaacggcgc gcctgtagag
     7621 cccatcacgg agcggcgggc gcgctcaggc cccgatactg cagcaacggc ccgatgtccg
     7681 gcgggccgcc gtagaagtcc cggaacagca cctgaggctc ggagctgcgc ccacgcgaga
     7741 ggaccttggc gcgcaggata tcgccgttgg cgcgcgtgag cccaccgtgc tcatgcagcc
     7801 acttgcccgt gtcgcgagca agcacctcgc tccacaggta tgcgtagtag gcggcggagt
     7861 atccgccggc gaagatgtgc gagaaatagc tcgaatgata gcgagtcgcg atcggcggat
     7921 agtcgattcc aacctcgtgc agggcgcgcg tctcgaatgc cgccacctcg gccgccgccg
     7981 gggcctgtgc ggggttgatc tggtgccagg actgatcgag cagcgccgcc gccaggtatt
     8041 ccgtaagcgc atacccctgg ttgaacttct gtgccgccag gaccttctcg agcagggcct
     8101 caggcatcgg cgcgccgctg cggtaatcgc gggcaaagtg cgccaccacg gccggctccc
     8161 gtgcccacat ctcgttgtac tgcgagggat actcgacgaa gtcgcgcggc acgctggtcc
     8221 ccgagagcaa cggatactgc acgctcgaga gcatggcgtg caccgcgtgc ccgaactcgt
     8281 ggaacagccc ggtgacctcc tcgaacgtca gcagcgcagg ctgaccggcc tgcggcttgg
     8341 gaatattgag gttgttgccc accaccggct tgcgcccgag cagcttcgac tggcgcacga
     8401 agctgttcat ccacgcccca ccttgcttgt tgtcccgtgc gaaatagtcc ccgaggaaca
     8461 gcgcaagcgg tgagccgtcg gcatccaaca cttcaaacac ccgcacgtcg ggctgataga
     8521 ccggcagatc cttacgctcc ttgaatgtca gcccgtagag ctcgtgtgcg gcatggaaga
     8581 ctccgtcctg cagcacatgg tccagttcga agtatggctt gacctgcgac tgatcgaaat
     8641 cgtagcgcga agcgcgcagc ttctccgcgt agaactccca gtcccatggc tgcaacgcga
     8701 acgtcgcagt gtgacgcacg gccgcttcgg catcgatgat catctgcagc tgtcgagcat
     8761 cgtctttcgc acgcgcgagc gcggccggca cgagctgacc gagtatctgg tgtacggccg
     8821 cagaggaagc cgccgattca tcagcgagaa cgtacgcggc gtgattgggg tagccaagca
     8881 aagcggcgcg ctcggcacgc aatctgacta tctgcgcgat cacgcccctg ttgtcggcgc
     8941 ctgggccact gcccgaggac accccgaggc cgcgtcccgc cgaggccttg tagatccgct
     9001 cgcgcaagcc gcggttcgtg agctgcgcca gcagcggctg gttcgtcgtg ttctgcagcg
     9061 gaatcagcca ttttccgggc attccccggg cctgcgcggc aagcgccgca gccccggcct
     9121 gctcggccga caagccatcg agctcggcta ccctgtccac caggacggca ccgtcctgcg
     9181 tcgccttgag cacgttctgc ttgaaacggg tggtgaggcc ggagagctgc tcgttcagcg
     9241 agcgcaggtg ctgcttgtcc gtttccgaca gctgtgcgcc ggcgcgcacg aactcggtgt
     9301 gatagcgctc cagaagctgt gcggactcgg catcgagctg caggcccgag cgcgtgcgat
     9361 acaccgcatc cacccgggcg tagagcgcgg aatccaggaa aatcgcatcc tcatgcgact
     9421 gcaatcgcgg cgcgatctcg gactcgatcc tctgcatctg cggattggtg ttggaagcat
     9481 tgaggttgga gaacgcgctc gagacgcgct ccagcagctc tcccgagcgc tcgagcgcga
     9541 ccagcgtgtt ctcgaacgtc ggcgcgttgc gcgcttgggc gatctgcgtg acttcatcgc
     9601 gctgagcgcg catgccggcc tcgaaggcgg gcaaatagtc cgtgtcgtgg atccgatcga
     9661 acggaggcaa ctggaacggc agcgtactcg gccgcgcgaa tggattgtca gatggaaggt
     9721 cggccatggg cgttgtcctg gatggcgcgg cgtacacctg cgtggacggg ctgaggctgt
     9781 gcaggcgagc aggagcggcg ctgcacgccg gcagcggcgc gacgccccgc cggaggcgtc
     9841 atggcgcgag ctgctcttca ccggataccc agcggcctat cttcccatac gggtctggcc
     9901 ccggcagaac tgttcccgct cccgcgcgct cttcgctaca cttgcggggc gcaccttacc
     9961 gggatctcat cttgagcatc aagcaggact ccgtcgtcac cttccactac acgctgcgcg
    10021 atgatgccgg tgagatcatc gaccgctccg atgccgggca gcctctctcc tacctgcagg
    10081 gtcatggcaa tctggttcca ggactcgagc aggcactcga gggcaagagc gccggggaga
    10141 agctgagcgt caaggtctca ccggcgcagg gctatggcga atacagccag gatctgttgc
    10201 agcaggtccc gcggcgttct ctcaagggca tcggtcggct cacccctggc atgcgcctgc
    10261 acgcgcagac cgcgcaggga ccgcggacgg ttaccgtcac gcgtatcacg ggagacatgg
    10321 tggctctcga tgcgaaccat ccgctggccg gcaagaacct gaacttcgac atcgagatca
    10381 ccggggtgcg cgaagccacg caagaggagc tcgcgcacgg ccacgtccat gggcccggcg
    10441 gccatcatca ctgaacgcac tgcatgagcc gcccgcgcgc cctctacgca gcctcgttgg
    10501 cattggcgct gctgagctgg accggcgcga gcctcgcaag ccggtgtccg acggcgccgg
    10561 aatccgagaa gaaggcaatc ctcgagctga tcaaccgcat gcaggatgcc tggaaccgtg
    10621 gcgacttccg cggctacatg cagggcttca agaatccgga tgtgatcttc gtctccggtg
    10681 ggcgcttcca gtccggatgg cagggtacgc tcgatcacta tgtacgcgac tacggcgcat
    10741 cggccgaaac acgcggccgc ctgcgtttct tcaacatcag gatcgagatg ctggcgccgg
    10801 atgccgcgca actcatcagt cagttcgagc tcttgcggcc gcagcaccct cagtacggca
    10861 tcaacacgcg tctgatgcgc aaggttgacg gccgctgggt gattgcgctc aaccacgtct
    10921 ccgccagcga gactccgccg cagcgttaga ggtgtcgaaa ggacggtgaa gtgcggcacg
    10981 ccgtgccgca cgggacagtt cagggcggcc tcgagtgcgc gacccacccc cgccagagag
    11041 acacgagcgc tgccatcagg gcagggccga gaaaaatgcc gatcagcccg aaggactcga
    11101 tcccgcccag gattccgagc agcacccaca ggaacggcaa cttggcacct tcaccgatga
    11161 tgagcgggcg tacgaagtga tccgcgatga agagcatcac gaacccgcag acgagcacac
    11221 cgatggcggg gccgacagcg ccctgactga acagcagcag ggccacgatt ccgaatgcca
    11281 cgggcgcggc gaatggaacc atggcaaaga tacccgtcac caccccgatc aggatcgggt
    11341 ggcgcacgcc ggtgacggcg tagacgcccg ccatgatcgc accttcagcg actgcaacca
    11401 gcacgatgcc atccacggtc gcgcgaacgg cttccgcggc gcgggtgagc aggctctcca
    11461 cggtcgggcc gaacagccga cgactcacgc gcggcacatc actacgcaag ccatcccggt
    11521 tcaaatacac gaagtacagg gtcagcaagg tgaaaacgag cgtactcagg cgccgcagga
    11581 gctggacacc gaccatgcgc gtccattcgt acacggccgg cttggctggc gcggcagcgg
    11641 cacccgcgtg atgcacctgt tccagccaca tgtcgtgaat ccagcctccg acgagcggca
    11701 actgcaccac ccattgaggc agctcgggcg gcccgccctt ggaggactcc aggtaggcgc
    11761 gcaccagtga gatcgcttcg cgcacggcta ccacaccccc gtataccagg ggcacgatca
    11821 acacgatgcc gacaatggcg gtgaagctga gtgctgccca gatgtgacgg tgcttgccgc
    11881 gaaactttgc gagccaccgg tcatacagcg aggaggttgc aatcgcgagc accacggccc
    11941 agccgagcgc cgccaggaaa tgctgcaaca tccaggcgcc gagcgcgacc agcgagccga
    12001 tcagcgcata gcgtgcaatc ttctgagcga cggagacgtc tggtgtggac acgctgcgtc
    12061 tttgcgaagg gatacagcga gtatacaggc gtttatactc gcgaagatgg ctccaaccgc
    12121 cgaaccgctc agccgcgcgc ccagcccctc gccaggacct gcagccgagc gcatcggttg
    12181 tccggattgc ggcctgctgc aaaggttacc gctgccagga cggggccagc tcatcgagtg
    12241 cgcccgctgt gggcgcaccc tcgcgggccc tgcgacgggg caggtgtcta cgccgtttgc
    12301 gctcacggtg agcgccttgc tgctgctgtt gccggccctg atcgagccgc tcatgacggt
    12361 ctccagctat ggtgcgcacc gttccatcgc tctgccggcg agtgccgagg ccctgtggca
    12421 tgacggtttc ccttcactgg gactgctcgt gggcgccttc agcatcgcgc tgccgctggt
    12481 gttcctgtct ctgctcatct gggtgtttgc caggctgcaa atgggcagta cgcagggcgt
    12541 gggcacggcc ttccgctgga ccaggcacct gcgtccctgg gtgatgatcg aggtgtacct
    12601 ggtcggcggg ttggtggcgt acagccggat caacatgatg acttcggtcg aaatcggcag
    12661 cggtgggtgg tgtctggtcg cctcctcgct ggtgctgctc gtcgccttga tcgggcttga
    12721 tgagcgaacg gtctgggaga ggctcgctcg gcctgttccc cccgcaacgg gcagcacgct
    12781 ggcctgtagc gtgtgcgacc tcatcgtcgc acccgatgcc acccgctgtc cccgctgcca
    12841 cgcctcgctc gctgtccgaa agccgcactc gctgcagtgg actgcggctc tcgtggcggc
    12901 gggctacctg ttatatgtgc ccgcgaacct gctcccggtg ctgaccacgg tgcgcctggg
    12961 tcgggaggag cacaacacca tcctgagcgg gatcatcgag ctggcgcgca acgatctggt
    13021 gccgctggcg gtgatcgtgt tcgcggccag catcgtgctg ccgatcgtga agctcttcgg
    13081 cctcacctgg atgctgctgg cgatacgctc ccgctcgggc ctctacctcg tcgggcgcac
    13141 cagactttac cgagtgatcg atgcgatcgg ccgctggtcc aacatcgatg tgttcgcagt
    13201 ggcagtgctg gtcgcactgc tgcagtttgg agagctcacg gccgtgcacg cgggcaacgg
    13261 cctggtcgcc ttcgccgctg tggtcgtcat cacgatgctc gcgaccatgg tgttcgatgc
    13321 tcgcctgatg tgggatgcag tccggagtag gcatgaacga aggccctgag gagcgcgagc
    13381 aggaggaaga gccccgggcc cgcctgcgcc cgcatggctg gttcggctgg gcatggctga
    13441 taccgatcgg ggcggctgca gtgctcctct ggctcgcctg gcgtggcctc gccgagcgcg
    13501 ggccggggat cacgatctcc ttcaggaatg cggagggtct gcaggccggc cagaccaaga
    13561 tccagcaccg caacgtcgat gtgggcacgg tcgaatccct cgagctgact ccggacatgg
    13621 cccacgtcat cgtacatgcg cggatgacac gtgtggccag cgatcatctg acagccaaca
    13681 cgcgcttcgc gatcatcgcc ccgcacgtgg gagtcggcgg tatctccggg ctctcgacca
    13741 tcgtttcggg ctcctacatc gagatgtatc ccggtacccc gggcccgccg cagcgccagt
    13801 tcgtgggcct gga
//
LOCUS       DASRHN010000131         4545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_208028, whole genome shotgun sequence.
ACCESSION   DASRHN010000131 DASRHN010000000
VERSION     DASRHN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208028"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFB37_05040"
     CDS             complement(<1..825)
                     /locus_tag="VFB37_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21850.1"
                     /translation="MAKSEPWVVHKFGGSSVADADCFRRVATILDSLPAGRLAVVLSA
                     CRGVTDALLRLVSLAEEQDEAYLGEIAALRVRHAAIAEALLRPESARLYLAGFDRDCH
                     DLEGILHTVKLTRAAARNVSDLIAGYGEIWSTRLFHRFLEERGGREGPFQWLDARRVV
                     LVKWGPLGPGVQWTESSERLTELLDPDFTGTLVITGFIASDRRGVQTTLGRNGSDFSA
                     SVFGALLGAREIHIWTDVDGVLSADPRRVPDATVIDSLSYNEAMELAYFGAKVIHPQ"
     gene            952..2049
                     /locus_tag="VFB37_05045"
     CDS             952..2049
                     /locus_tag="VFB37_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZO21851.1"
                     /translation="MESLLDPLLPLYERERAANRAVALGVLVQTLGSTYRKAGAMMLI
                     AADGDYAGLLSGGCLEGDLREHARAVIETGQARLIEYDTRGGDDVIWGLGLGCEGAIT
                     ILLLRAGPEQSWEPLQHLADSLSQRRRTAVGLVVQSERSELPAGSCILPGAERRERGL
                     MEAIAGSADTGRPGWFESDSPRYRVFAVPLTLPPRLLLLGAGPDAFPLVDFAARLRWS
                     ITVVDHRPAYAAADHFPGAEQALLARAEELDEKLDLSSYSAAVVMSHHLPTDLTYLRV
                     LSRTPVPYIGLLGPANRRERLMSDLGPDAMSVRARLRAPVGLAIGGRTPEAVALSIVS
                     EIHAFLHAAEGGTPTTRDVPTAADKARSVPA"
     gene            2046..2471
                     /locus_tag="VFB37_05050"
     CDS             2046..2471
                     /locus_tag="VFB37_05050"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21852.1"
                     /translation="MNEIQIIQQHLAIERQHFAQVVAACTTALAAPRAAHEPLIEACA
                     QYFEFAVTRLQPDAIAAARARLQAARKADPDRLSEHWQAFLQMFTAACNERFATIDEL
                     AKRNESVAQWRNIGRLDAESIVGERERFARVQAGLSGAP"
     gene            2468..3073
                     /locus_tag="VFB37_05055"
     CDS             2468..3073
                     /locus_tag="VFB37_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZO21853.1"
                     /translation="MTEPVGTEDALHAIVLAAGASTRFGSVKQLVRIGGRPLLHTVVA
                     RACEVVGSAVTVVLGAHAAELAPLLTHTPATIVINRNWREGMASSIRAGITRLAPSCS
                     AVLLVLADQAAVTVEDLRRLAGAWRRQPEYMAAARYGTTTGVPAIFPRSTFGELIALR
                     GDVGARALLQRNPDRVVRVPIASAAIDIDTPEDLLALDPSK"
     gene            complement(3227..4183)
                     /locus_tag="VFB37_05060"
     CDS             complement(3227..4183)
                     /locus_tag="VFB37_05060"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZO21854.1"
                     /translation="MTELTPPPGVRRVGTSPPLRSLLFVPGDSERKQTKALASAADLL
                     ILDLEDSVASAHLPAAREQVRELLLGNTERSAQQLWVRLNALSSGLLLQDLAAVFPGQ
                     PDGIVLPKASSAEEVSEVSHYLSAMEAQAGRPIGSTRLIVIVTETPQALLRLGEYPRA
                     SGLTASARERLAGLTWGCEDLSAALGITHKTDRQGSLTFTFQLARSMCVLAAAAVGVQ
                     AIDGVFLDYRDLEGLKRDIESARRDGFTGKLAIHPDQIEAINAAFLPAASEIEWARRV
                     VAAFASDPEAGVLSVAGQMVDRPHLVQARRILELVARPRGTP"
     gene            complement(4180..>4545)
                     /locus_tag="VFB37_05065"
     CDS             complement(4180..>4545)
                     /locus_tag="VFB37_05065"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZO21855.1"
                     /translation="FSTLTMNPAALHLDAEYCKGTPFGKPVVNSVFTLGLLVGLSVYD
                     TTYGTTLGNLGWEEVRFPRPVFVGDTLRAQTTVVAKRESQSRSDSGILTFEHRAYNQR
                     NEEVASCRRAALMMKRPAA"
BASE COUNT          752 a         1532 c         1508 g          753 t
ORIGIN      
        1 ctgcggatgg atcaccttcg cgccgaagta cgccaactcc atggcctcgt tgtacgagag
       61 ggaatcgatc acggtcgcat ccggcacgcg tcgggggtcg gcagacagca ccccatctac
      121 atccgtccag atgtggatct cgcgggcgcc gagcagcgct ccgaacacgg aggcggagaa
      181 atcgctgccg ttgcggccga gggtcgtctg cacgccgcgc cggtcggagg caatgaagcc
      241 ggtgatcacg agcgtgccgg tgaaatccgg atcgaggagc tcggtgagcc gctcgctcga
      301 ttctgtccat tggacccccg gtcccagcgg accccatttc accagcacca cccggcgggc
      361 atcgagccac tgaaacggac cctcgcggcc cccgcgctcc tccaggaacc gatggaaaag
      421 ccgggtggac cagatctcgc cgtagccggc gatcaggtcg ctgacattgc gagcggccgc
      481 tcgcgtcagc ttgaccgtat gcaggatgcc ctcgaggtcg tggcaatctc ggtcgaagcc
      541 tgcgaggtac aggcgcgcgg actccggccg cagcagcgct tccgcgatcg ccgcgtgccg
      601 tacccgtagc gcggcaattt cgcccaggta ggcctcgtcc tgctcctctg cgaggctcac
      661 caggcgcagc aacgcatcgg tcacgccccg gcacgccgag agcacgacgg cgaggcgccc
      721 cgccggcagt gaatcaagaa tggtcgcgac ccggcggaag caatcggcgt cagcgacgct
      781 cgaccctccg aacttgtgta cgacccaggg ttcgctcttg gccatctaga acgggaacag
      841 ggtggaaaaa gcgatgcgac tgtactgacg cgcttcagtg cacgcaatga cggggtcgtg
      901 cctttgtgtg gcggaggcgc gccgtgcggc cttgtcgata taatccaccc gatggaatcg
      961 ctgctcgacc cgctgctgcc gctctacgag cgtgagcgcg ccgcgaaccg cgccgtggcg
     1021 ctcggcgtgc tcgtccagac tctgggctcc acctaccgca aggccggggc gatgatgctc
     1081 atcgccgcgg atggggatta tgccggcctg ctctccgggg gctgcctgga gggcgatttg
     1141 cgcgagcacg ctcgggccgt gatcgagacc ggtcaggccc gcctgatcga gtacgacacc
     1201 cgcggcgggg acgatgtgat ctggggcctg ggactcggct gcgagggcgc gatcacgatc
     1261 ctgctgctgc gcgcaggacc ggagcagagc tgggagcccc tgcagcacct ggccgatagc
     1321 ctctcgcagc gtcgccgtac tgcggtggga ctcgtggtgc agtccgagcg gtccgagctg
     1381 ccggccggga gctgtatcct gccgggagcc gaacggcgtg agcgcgggct gatggaggcc
     1441 atcgccggct cagccgatac gggccgcccg ggctggttcg aatccgatag cccccgctac
     1501 cgggtattcg ccgtgccact gactctgcca cccaggctgc tgctgctggg cgccgggccc
     1561 gatgcgtttc cactggtgga cttcgcggcg cggctgcggt ggagcattac ggtggtcgat
     1621 catcgtcccg cttacgcggc cgctgaccac ttcccggggg ctgagcaggc cctgctggcg
     1681 cgagcggaag agctcgacga aaagctggac ctgtcgagtt attccgctgc cgtggtgatg
     1741 agtcatcatc tgcccacgga ccttacatac ctgcgggtgc tctcgcgtac gccggttccg
     1801 tacatcggtc tgctcggccc ggccaaccgg cgcgagcggc tgatgtcgga tctcggcccg
     1861 gatgcgatga gcgtgcgcgc gcgactgcgc gctcccgtgg ggcttgccat tggcggacgg
     1921 acccccgagg cggtcgccct ctccatcgtg tcggagattc atgctttcct gcatgcggcc
     1981 gagggcggta ccccgacgac ccgcgatgtg cccacggccg ccgacaaagc ccgctccgtc
     2041 ccggcatgaa tgagatccag atcattcaac agcatctcgc catcgaacgt cagcactttg
     2101 cgcaggtagt cgccgcctgc acgaccgctc tcgcagcgcc gcgggccgca cacgagccgc
     2161 tgatcgaggc atgcgcccag tacttcgagt tcgccgtcac acgcttgcag cccgatgcga
     2221 tcgctgcagc gcgggcaaga ctgcaagctg ctcgcaaggc cgatccggac aggctttctg
     2281 aacactggca agcgttcctg cagatgttca ccgcagcgtg caatgagcgc ttcgcaacca
     2341 tcgacgagct ggcaaagcgc aacgagtctg tggctcagtg gcgcaacatc ggccgcctcg
     2401 atgcggagag catcgttggc gagcgcgagc gctttgcccg ggtgcaagcc ggactgtccg
     2461 gggcaccatg actgagcccg tcgggaccga ggacgcgctg cacgcgatcg tgctggcagc
     2521 cggcgcttcg actcgattcg gatccgtgaa acaactcgtc cgcatcggtg gtcggccgct
     2581 gctacataca gtcgtcgcgc gcgcatgtga ggtagtcggc agcgcagtca cggtagtgct
     2641 cggtgcgcat gcggccgaac tggcaccact gctcactcat acccccgcca cgatcgtgat
     2701 caaccgcaac tggcgtgagg gcatggcgag ctcgattcgc gccgggatca cgcgcctcgc
     2761 cccgagctgt agtgccgtac tgctggtgct ggccgatcag gccgccgtga cggtagagga
     2821 tctcaggcgt ctcgccggag cctggcggcg ccagcccgag tacatggccg cggctcgcta
     2881 tggaaccacc accggcgtcc cggccatttt tccgcgctcg actttcggtg agctgattgc
     2941 gcttaggggg gacgtcgggg cgcgagccct cctgcagcgc aatccggatc gtgtggtgcg
     3001 cgtgcccatc gcgagcgcag ccatcgacat cgatacaccc gaggaccttc tggcgctcga
     3061 tccctccaag tagccgcagt cgccggactg ctccgggccc cgatgcaccc ttcaacgggc
     3121 cgtggcagtg ccggccccaa tgtcgtgcac catggaggtt tgcatctcga gaagctgcat
     3181 cttgtcgcga tcttggcgtc cgcgtgcgtg agggtcgagc cagccattat ggtgtgcccc
     3241 gcggccgcgc gacgagctcg aggatgcgcc gggcctgcac cagatgcggc cgatcgacca
     3301 tctgccccgc cacgctcaac acgcccgcct ccggatcgct ggcaaaggcg gccacgaccc
     3361 gccgcgccca ttcgatctcg gaagccgcag gcaggaaggc tgcgttgatg gcttcgatct
     3421 ggtccggatg gatggcgagc ttccccgtga agccatcgcg ccgcgcactc tcgatatcgc
     3481 gcttcaaccc ttcgagatcc cgataatcca ggaacactcc gtcgatcgcc tgcacaccga
     3541 ccgccgcggc ggccagcacg cacatcgagc gcgcaagctg gaaagtgaag gtcagcgagc
     3601 cctgtcggtc cgtcttgtgc gtaatcccga gtgcggcaga cagatcctcg caaccccatg
     3661 tgagcccggc cagccgctcg cgtgccgagg cggtgaggcc cgatgctcgc ggatattcgc
     3721 cgagccgcag cagcgcctgc ggtgtctcgg tcacgatcac gatgaggcgc gtggagccga
     3781 tgggccgtcc tgcctgcgct tccatcgcag agaggtagtg agaaacctcc gagacttcct
     3841 cggcggagga ggccttcggc agcacgatgc catcgggctg tcccgggaat acggcggcga
     3901 gatcctgcag cagcaggccg ctcgagagcg cgttgagccg aacccagagc tgctgggccg
     3961 atcgctcggt gttgccgagt aacagctcgc gaacctgctc gcgtgcggcc ggcaggtgcg
     4021 cgctggccac cgaatcctcc aggtccagaa tgaggaggtc ggcggcgctt gcgagcgcct
     4081 tggtctgctt tcgctcgcta tcccccggga cgaacagcag cgagcgcagc ggcgggctgg
     4141 tccccactct cctgacccct ggaggcggtg tcagctcagt caagcggccg gcctcttcat
     4201 catcagagcc gcgcgccgac aggaggcgac ctcctcgttc cgctggttat aggctcgatg
     4261 ctcgaaagtc agaatgccgg aatccgaacg ggactgactc tcgcgcttcg cgaccacggt
     4321 cgtctgggca cgcaatgtgt cgccaacgaa gaccggacgc gggaagcgca cttcctccca
     4381 gcccagattg ccgagcgtgg tcccatacgt ggtgtcgtag acagacaatc cgacgagcag
     4441 ccccagcgtg aagacgctgt tcaccacggg cttgccgaag ggagtgccct tgcagtattc
     4501 ggcatcgaga tgcagggccg ccgggttcat ggtgagcgtg ctgaa
//
LOCUS       DASRHN010000132         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1362543, whole genome shotgun sequence.
ACCESSION   DASRHN010000132 DASRHN010000000
VERSION     DASRHN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362543"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="tsaD"
                     /locus_tag="VFB37_05070"
     CDS             complement(<1..661)
                     /gene="tsaD"
                     /locus_tag="VFB37_05070"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZO21856.1"
                     /translation="MRRPAKAKDVLILAIESSCDESAAAVLDSGRGLLAHELFSQVEL
                     HRAYGGVVPELASRDHVRRLLPLVRRALEQAGSDPKELHGVAYTAGPGLIGALLTGAA
                     LARSLAYAWQIPAIGVHHLEGHLLAPLLEPAPPPFPHVALLVSGGHTMLIEVCNIGRY
                     ELLGQTRDDAAGEAFDKTAKLLGIAYPGGPQLAQLAERGRPGAYDFPRPMLDRPGLEF
                     SF"
     gene            765..974
                     /gene="rpsU"
                     /locus_tag="VFB37_05075"
     CDS             765..974
                     /gene="rpsU"
                     /locus_tag="VFB37_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZO21857.1"
                     /translation="MPSVRVRENEYFDAALRRFKRACEKAGILTELRRREFYEKPTQE
                     RKRKKAAAIKRHMKRISRDGMRAAR"
     gene            1055..1501
                     /locus_tag="VFB37_05080"
     CDS             1055..1501
                     /locus_tag="VFB37_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZO21858.1"
                     /translation="MPLKERITEDMKAAMRSGDKERLATIRLILAAVKQREVDERISL
                     DDNQVLAALEKMIKQRKEAITQFQAGGRADLVAKETAEVAVLQGYLPAQMSEAELDAL
                     ISEAIAATGAASVKDMGKVMGVVKSKAQGRADMGAVSARIKQKLSG"
     gene            1648..3459
                     /gene="dnaG"
                     /locus_tag="VFB37_05085"
     CDS             1648..3459
                     /gene="dnaG"
                     /locus_tag="VFB37_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZO21859.1"
                     /translation="MIPQTFIDELIARADIIEVIGLRVQLKKAGREYKACCPFHNEKT
                     ASFWVSPDKQFYHCFGCGAHGTVIRFLMEHDHLTFPEAVEELAGRLGLEVPQEGLSSG
                     TIRRPDEPLYDLMGRVARLYSDSLTRDERAQAYVARRGLDAETLAGFGIGYAPNSWSE
                     ILRRFGATEDERRALSDLGLIVERDRAQTRTAERFYDRFRDRIMFPIRDSRGRIIAFG
                     GRILDQGEPKYLNSPETLLFHKGRELYGLYEARRNRSNLRRLLIVEGYMDVVRLHQFG
                     IGYAVGTLGTATTPEHLKRIFRIVNEIVFAFDGDRAGRAAAWRALQNTLPEIRQGREV
                     RFLFLPDGHDPDTLIGEEGAEAFEKRLESTLPLADYLMRELQEQSGELDEQGRARFSQ
                     NAGPLMSRIPDSVYRDLLIERIGRVVRITPERLEQIWREANRGRAADGAAAQFAPELG
                     RGARTGVSAGRGGLVRQAILLLLTFPPVAGEVTETERNGLADCDEPGIELLRQLLAEL
                     QERPARSPAQLIERWAGRPEHEILAKLLQREQVVADAVAAADELRAALRKLADRVAIR
                     RLEALEAKEVATGLDAQEKQEYLRLTMLRASPGSRGG"
     gene            3681..>4185
                     /locus_tag="VFB37_05090"
     CDS             3681..>4185
                     /locus_tag="VFB37_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HZO21860.1"
                     /translation="MPTVREKDRSKAAITGRKAAAVDAGSHAPETSRPAEAKGSGPKS
                     AVEKKPLGIERRVNVMPEDRQSQLKLLIARGKEQGYLTYAQVNDHLPSEIVDPEQIED
                     IVNTINDMGIPVYEKAPDTESLLATEPSQPADEEAIEEAAAALAALDAELGRTTDPVR
                     MYMREMGT"
BASE COUNT          763 a         1348 c         1406 g          668 t
ORIGIN      
        1 agaagctgaa ctccagcccc gggcggtcga gcatcggacg gggaaagtca tacgcaccgg
       61 ggcgccctcg ctcggccagc tgcgccagct ggggtccgcc cggataggct atgcccagca
      121 gcttggcggt cttgtcgaaa gcctccccgg ccgcatcgtc ccgagtctgg cccaggagct
      181 catagcggcc aatgttgcag acctcgatca gcatcgtatg gccgcccgag acgagcagcg
      241 ccacgtgggg gaaggggggt ggcgcaggct cgagtaacgg cgccagcaga tggccctcga
      301 ggtgatggac gccaattgcc ggaatctgcc atgcgtaggc caggctcctg gcgagtgccg
      361 cgccggtcag gagtgcgccg atcagccctg gacctgccgt gtaggcgacc ccgtgcagct
      421 ccttcgggtc gctgccggcc tgttccaggg cgcggcggac caggggaagc agccgccgga
      481 catgatcacg cgaggccagc tctggaacca ctccgccgta ggcgcgatgc agctccacct
      541 ggctgaacag ctcatgcgca agcaggcccc gcccggaatc gaggacggcg gccgcacttt
      601 cgtcgcagga ggattcgatg gcgagaatga gcacgtcttt tgcctttgcc gggcgcctca
      661 attagaatcc cccgcccttt gtcccaggga ctcttcgagg ggcggactgg ctttccggaa
      721 aaggtccatc ccggagccgc caccccagcg taactgaggt ttcgatgccg agcgtccgtg
      781 ttcgtgagaa cgagtatttc gatgccgcct tgcggcgttt caagcgcgcc tgcgagaagg
      841 ccggcatcct caccgagctg cgtcggcgcg aattctacga gaagcccact caggagcgga
      901 aacgcaagaa ggccgccgcg atcaagcgcc acatgaagcg catttcgcgt gacggcatgc
      961 gcgccgcccg gtaagactgc gctgggcacc gcttccacgt cggtgccgct ggggattcga
     1021 caggccgcca cccgccacgc ccgcgccacc cgccatgccg ctgaaagaac gcataaccga
     1081 ggacatgaaa gccgcgatgc gctccggcga caaggagcgg ctcgcgacca ttcgcctcat
     1141 cctcgctgcc gtcaagcagc gggaagtgga cgagcgcatc agcctggatg acaaccaggt
     1201 gctcgcagct ctcgagaaga tgatcaaaca gcgcaaggag gcgatcaccc agtttcaggc
     1261 cggcggccgg gcggatctcg tcgccaagga gacggcggag gtagcggtcc tgcagggcta
     1321 tcttccagcg cagatgtccg aggccgagct ggacgcactg atcagcgaag ccatcgcggc
     1381 gaccggcgcc gcctcggtca aggacatggg caaggtcatg ggcgtggtga agtcgaaggc
     1441 ccagggccgg gccgacatgg gcgcggtgag cgcccgaatc aaacagaaat tgagcggctg
     1501 agtccccgat gggtgccgcc gccgcggccg gtgctatcta cgaggcggct cgctcgcgcc
     1561 tttccgccgc aagcgcgtgc ggttggcgct ctcgggcggg ctgatcgcgc tgctgctggc
     1621 ggtcgtggtt gcgttacgct tgaccctgtg atcccgcaga ctttcatcga cgagctgatt
     1681 gcccgcgcgg acatcatcga ggtcatcggc ctgcgcgtgc agctcaagaa ggccggtcgc
     1741 gagtacaagg cctgctgccc gtttcacaac gagaagacag cctcgttctg ggtgagcccc
     1801 gacaagcagt tctatcactg cttcggctgc ggtgcgcatg ggacggtgat ccgcttcctc
     1861 atggagcacg atcacctcac cttcccggag gccgtcgaag agcttgccgg gcggctcggt
     1921 ctcgaggtgc cacaggaggg cctgtcgagc gggacgatcc ggcggccgga tgagccgctc
     1981 tacgatctca tgggacgcgt tgccaggttg tattcggact cgctgacacg ggatgagcgt
     2041 gcccaggcgt atgttgcgcg tcgcggtctc gacgccgaga ccctcgccgg cttcggaatc
     2101 ggttatgcac ccaactcctg gagcgaaatt ttgcgccgct tcggtgccac ggaggacgag
     2161 cggcgtgcgc tgtccgatct cggcctcatc gtcgaacgtg accgggcgca aacccgcacc
     2221 gccgagcggt tctacgaccg cttccgcgat cggatcatgt ttcccatccg cgacagccgg
     2281 ggtcggatca tcgccttcgg cgggcgcatt ctcgatcagg gcgagccgaa gtatctgaac
     2341 tctcccgaga ccctgctctt tcacaagggg cgcgagctct acggactgta cgaggcgcgg
     2401 cgcaaccgtt ccaacctgcg gcgactgttg atcgtcgaag gctacatgga tgtcgtgcgc
     2461 ctgcatcagt ttggaatcgg ctatgcggtc ggcactctcg gcaccgcgac cacgcccgag
     2521 catctgaaga gaattttccg catcgtcaac gagatcgtgt tcgcattcga tggtgaccgg
     2581 gccggccggg cggccgcctg gcgcgcgctg cagaacacgt tgcccgagat tcgccaggga
     2641 cgcgaggtgc ggttcctgtt tttgccggat ggccacgacc ccgacactct gatcggggaa
     2701 gaaggcgccg aggcattcga gaagcgcctc gagagcacgc tgccgctcgc tgactacctg
     2761 atgcgcgagc tgcaggagca gtccggtgag ctggacgagc agggccgggc acgcttttca
     2821 cagaatgccg ggccgctcat gagccggatc cccgacagcg tctatcgcga tctgctcatc
     2881 gagcggatcg ggcgcgtcgt gcgcatcaca cccgagcgcc tggaacagat atggcgggaa
     2941 gccaacaggg ggcgcgcagc ggacggcgcc gcggcacagt tcgcgccgga gctcgggcgt
     3001 ggcgcgcgaa caggggtgag tgcgggacga ggcggtctcg ttcgccaggc cattctgctg
     3061 ctgctgacat tcccaccggt cgctggcgag gtgaccgaaa cagagcgcaa cgggctcgcg
     3121 gactgcgacg agcccgggat cgagctgttg cggcagctgc tcgccgagct gcaggagcgg
     3181 ccggcacgct cgcccgccca gctcatcgag aggtgggccg gaaggccgga acacgaaatt
     3241 ctcgcaaaac tgcttcagcg cgagcaggtc gtggccgatg cagtggcagc agccgatgaa
     3301 ttgcgggctg cgctgcgcaa gctggccgat cgggtggcga tccggcgcct cgaggcgctc
     3361 gaggccaagg aggtcgcgac cggcctggat gcgcaggaaa agcaggaata tctgaggctt
     3421 accatgctaa gagcctcccc gggcagccgt ggaggctagc gcggaatttg gtgtttgggc
     3481 ccttgtcaaa tagaatcgca cgctttgctt tggcgcccct tgagttgcgg ctctgagacc
     3541 tgcacatagg ggatcgtccc ccgtcgcccc cgcaagccat gacacgcaag cacaaatccc
     3601 ctgcccctgc caagaagcgt ccttccgctg agtcgcgccg accggcgaaa gccgccgccg
     3661 ctgaaaagtc cgtagctgcc gtgcctacgg ttcgcgagaa ggatcgctcc aaggctgcca
     3721 tcaccggacg caaggcggcg gcggtagacg ctggttcgca cgccccggag acgagccgcc
     3781 ccgctgaggc gaaaggcagc ggcccaaaga gtgctgtcga gaagaaaccg ctcggcatcg
     3841 agcgccgtgt caatgtgatg cccgaggatc gccagtctca gctcaagctt ttgattgccc
     3901 gcggcaagga gcagggctat ctgacctacg cgcaggtgaa cgaccacctc ccctccgaga
     3961 tcgtcgatcc ggagcagatc gaggacatcg tcaacaccat caacgatatg ggcattccgg
     4021 tgtacgagaa ggcaccggac accgaatcgc tgcttgccac cgagccttcg cagcctgccg
     4081 atgaggaggc aatcgaggaa gccgccgcgg ctctggcagc gctcgatgcg gagctcggcc
     4141 gcaccaccga cccggtgcgc atgtacatgc gcgagatggg cacgg
//
LOCUS       DASRHN010000133         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2351235, whole genome shotgun sequence.
ACCESSION   DASRHN010000133 DASRHN010000000
VERSION     DASRHN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351235"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>3387
                     /locus_tag="VFB37_05095"
     CDS             40..>3387
                     /locus_tag="VFB37_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO21861.1"
                     /translation="MADEARVLARETHERSSGHLQLILDHIKDVVLTVDEEGIVQTLN
                     PTGERVFGHQAGEVVGRRIDLLIPKIASHESITQALHRLAASSGDTHLDLAARERWGQ
                     RKNGEFFPLEIAASKASLSRREMFVLCLRDITDRRQSEQAMRESEARYRLLVTHAPEA
                     IVVFDADAGRFVDANENAERLFGLARERLLEVGPVDLSPPLQSDGERSARRAREYVQR
                     ALDGEPQVHEWLHCDASGKEIICEVRLVRLPSGDRRLVRGSIADISERKRAERVAAAE
                     REVFRQLTGNASLQKVLTSITRLIESLGVGTVCSVSLLAEDGLSFSSMVAPQLPAILL
                     TQLEHAPIDIRNGSCAAAVYLSRQVLVADIAKDPFWQQRREPALAAGLRTAWSTPLKA
                     ADGKLLGSLGVYRSEPGLPTLRESEIMAHAAQLAGIAIQRRLAEEALRGSEAAFRGLF
                     ETMSEGVYQSSRDGRLLSVNPAFVSILGFGSAEELYALSNVALLYWNPGDRAEFMRRV
                     ESENEIRNAEFLMRSRDGEPVVVLENARARRDAAGRILGYDGTIADIRERKQAEQAIF
                     AEKERAQVTLQSIGDAVISTDAEGRIEYINPVAESLTAWSLEEARGRPIADVLNLVNE
                     ITREPIENPLMCALGRGQGGVSADHSVLITRTGQEVAIQESAAPICDRQGLVIGAVIV
                     FHDVTKERRLKRALSYQASHDALTGLINRREFDNRLHAAVLSAQRGEGSYALLYVDLD
                     QFKVVNDTCGHQAGDRLLRDVTGLLQTRVRASDTIARLGGDEFGVLLEGCTLDQATRI
                     ADGVRQAIRDYRFMWGTNQLSVGASVGVVQIRDDTQSVAAVMSAADIACYAAKDEGRN
                     RIHVYEQDGVSHHHREMHWVSRVTRAVEENRLELFFQPIEPIAADAGTAFHELTVRLR
                     DNDGQLVLPSEFIPAAERYNVMSIIDLWVIRQALELLKQRQSGPALPLLAVNLSGTSL
                     NDQTFTEFVLRALGETPLASALCFEVTETAAVTNLASAIYFMRELKARGCRFALDDFG
                     SGLSSFMYLKTLPVDYLKIDGQFIGQIAEDPVDRSMVEAISKVGAALGIATVAECVET
                     QAVLDELKRIGVDFAQGYFLAPPLPVAQ"
BASE COUNT          582 a         1063 c         1153 g          589 t
ORIGIN      
        1 gatcaggagc ggcgcggcgc ggcggaatcg atgcggctca tggccgacga ggcccgcgtg
       61 cttgcgcgcg agactcacga gcggagctcc gggcacctgc aactcatcct cgatcacatc
      121 aaggatgtgg tgctcacggt cgatgaggaa ggcatcgtcc agacgctcaa tcccacgggc
      181 gagcgtgtgt ttggtcacca ggcgggtgaa gtggtcgggc ggcgaataga tctactcatc
      241 cccaagatcg caagccacga gagcatcacc caggccctgc accgtctcgc agcgagctcc
      301 ggggacacgc atctggacct cgcggcgcgc gaacgctggg gccagcgcaa aaatggcgag
      361 ttctttcctc tcgagatcgc cgcaagcaaa gcctctctgt cgcggcgaga gatgttcgtc
      421 ctgtgcctgc gggacatcac cgaccggcgc cagtccgagc aggcgatgcg cgagagcgag
      481 gcccgctatc ggctcctcgt cacgcacgct cccgaggcca tcgtcgtctt cgatgccgat
      541 gccgggcgct ttgtagacgc gaatgagaat gccgagcgcc tcttcgggct cgctcgcgag
      601 cggctgttgg aagttgggcc ggtggatctg agcccgcctt tgcaatccga cggcgagcgc
      661 tcagcccgga gagcgcgcga gtacgtgcag cgcgccctcg atggcgagcc gcaggtgcac
      721 gaatggctcc attgcgatgc ctcgggcaag gaaatcatct gcgaggtgcg gctcgtgcgg
      781 ctgccgagcg gtgatcgccg cctcgtccgc ggcagcattg ccgacatttc cgagcgcaag
      841 cgcgccgagc gcgtggcagc ggccgagcgc gaggtctttc gccagctcac cggcaacgcc
      901 tcgctgcaga aggtcctgac atccatcact cgtctcatcg aatccctggg ggtcggaacg
      961 gtctgctcgg tgagtctgtt ggccgaggat ggcctgtcgt tttcgagcat ggtggcccca
     1021 cagctgcctg cgatattgct gactcagctc gagcacgccc cgatcgatat ccgcaacggg
     1081 tcctgtgccg cggccgtcta tctgagccgt caggtgctcg tagccgacat cgccaaggat
     1141 cccttctggc aacagcggcg cgagccagcg ctggcagcgg gactgcgcac ggcttggtcc
     1201 acgcctctga aggcggctga cggcaagctg ttgggctcgc tcggggtgta ccggagcgag
     1261 ccgggcctgc cgacattgcg tgaatccgag atcatggctc acgccgcgca gctcgcgggc
     1321 atcgccatcc agcgccgcct ggcggaagag gctctgcgcg gcagcgaagc cgcattccgc
     1381 ggactgttcg agaccatgtc tgagggtgtc taccagagct cgcgggacgg gcggctgctg
     1441 tcggtcaacc ctgcattcgt ctccattctg ggcttcggaa gcgccgagga actgtacgcg
     1501 ttgtcgaacg ttgccttgct ttactggaat cccggggatc gcgcggagtt catgcggcgg
     1561 gtggagtcgg agaacgagat ccgcaatgcc gagttcctca tgcgcagtcg cgatggtgag
     1621 ccggtagtgg tcctcgagaa tgcccgagct aggcgcgatg ccgcgggcag gatcctgggc
     1681 tacgacggaa cgattgcgga catcagggag cgtaaacagg cggagcaggc gatattcgcc
     1741 gagaaggagc gggcacaagt gacgcttcag tcgatcggtg atgcggtgat cagcaccgat
     1801 gcggaagggc ggatcgagta catcaatccg gtcgccgaga gcctgacggc ctggagtctg
     1861 gaagaggcgc gtgggcggcc catcgccgac gtgctgaatc tggtcaacga gatcacgcgc
     1921 gaaccgatcg agaatccgct gatgtgcgcg ctcgggcgcg gccagggcgg cgtgtcggcg
     1981 gatcactcgg tgttgatcac gcggacgggc caggaagtag ccatccagga atcggcggcg
     2041 ccgatctgcg accgccaggg actggtcatc ggtgccgtga tcgttttcca tgacgtgacc
     2101 aaggagcggc gactcaagcg cgccctgtcg tatcaggcca gccatgacgc gctcaccggc
     2161 ctgatcaacc gccgcgagtt cgacaaccgg ctgcatgcgg ccgtgctcag cgcgcagcgt
     2221 ggcgagggct cgtacgcgct gctgtacgtc gatctcgatc agttcaaagt cgtgaacgat
     2281 acctgcggcc accaggctgg cgatcgcctc ctgcgcgatg tgaccgggct gttgcagacc
     2341 cgggtgcgcg cctccgacac gatcgcgcgt ctggggggcg atgagttcgg cgtcctgctg
     2401 gaagggtgca cgctcgatca ggccacccgg attgccgacg gcgttcgtca ggcgatccgc
     2461 gactatcgct tcatgtgggg cacgaaccag ctctccgtcg gcgcgagcgt cggtgtggtg
     2521 cagatcaggg atgacaccca gagtgttgca gccgtcatga gcgctgcgga catcgcctgt
     2581 tacgccgcca aggacgaggg ccgcaaccgg atccatgtct acgagcagga cggcgtctcg
     2641 caccatcatc gcgagatgca ttgggtatcg cgcgtgacgc gcgcggtgga ggaaaaccgg
     2701 ctcgagctgt tcttccagcc gatcgagccc attgccgccg acgccggtac cgctttccac
     2761 gagttgacgg tgcggctgcg ggacaacgac ggtcagctgg tgctgccgag tgagttcatt
     2821 ccggccgccg agcgttataa cgtcatgtcg atcatcgacc tgtgggtcat tcgccaggcg
     2881 ctcgagctgc tcaagcagcg ccagagcggc ccagcgctcc cgctgctcgc ggtcaacctc
     2941 tcgggcacct cgctcaacga ccagaccttc accgaattcg tgctgcgggc gctcggtgag
     3001 acgccgctcg cctcggcgct gtgcttcgag gtcaccgaga cggcggccgt gacgaatctg
     3061 gcgagcgcga tctacttcat gcgcgagctc aaggcccggg gatgccggtt cgcgctcgat
     3121 gacttcggca gtggcctgtc atcgttcatg tacctgaaaa ccctgccggt ggactatctg
     3181 aagatcgacg ggcagttcat cggtcagatc gccgaggacc ccgtcgaccg cagcatggtc
     3241 gaggcgatca gcaaggtggg tgcggcgctc gggattgcga ccgtggccga atgtgtcgag
     3301 acacaggccg tgctggacga actgaaacgt atcggcgttg acttcgcgca gggctatttc
     3361 ctcgccccgc cgctccccgt cgcccag
//
LOCUS       DASRHN010000134         8976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_536171, whole genome shotgun sequence.
ACCESSION   DASRHN010000134 DASRHN010000000
VERSION     DASRHN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536171"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFB37_05100"
     CDS             <1..440
                     /locus_tag="VFB37_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21862.1"
                     /translation="LQGNIRAQLADSLVGVLCQRLDFLEAHHLLVPRCELLLASSAAR
                     GTIRAGQFSNIANVIQSGGEEGMWTFERYQRWMQQNKAWTRAAPSAATAEERPTAPEP
                     IAAPAPLGAAPGEITAPGEITIEEDMDLEELAELARQIEQRTS"
     gene            complement(469..1962)
                     /locus_tag="VFB37_05105"
     CDS             complement(469..1962)
                     /locus_tag="VFB37_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO21863.1"
                     /translation="MSSESAMQSGKGDRTRWWALLVLCMGVLMIVLDTTVVNVALPSI
                     RAQLHFSETSLVWVMNAYLLTFGGFLLLGGRLGDLFGHRRLFLSGVALFTLASLGCGL
                     ADSQGVLIVARAIQGVAGAVVSAVALSLIMNLFVEHEERAKAMGIYGFVCSAGGSLGL
                     LLGGVLTSSLNWHWVFLINVPIGIVVCVWVPRLLGKGSRPDSSPTLDIAGATTVTTSL
                     TLAVYAVVNGNETGWTSARTLGLLACAVVLFASFLLIESRARAPLMPLGLFRLRSLVG
                     ANIAATLWAAAMFAWFFISTLYLQLVLGAGPMQVGFAFLPANLIMAVFSLGLSAKLVV
                     RFGVKRPLAAGLLFAAAGLACFARAPVGGSILPDVVPGMMLLGVGAGMAFNPLLLAAM
                     SGVPARDSGLASGLVNTSFMMGGALGLAVLASLAAARTGAMLREGSVRVTALSSGYHV
                     AFAAGLICALAAGALAVILIPAEPPLPRRQGERVPAAGAPMSAAEGE"
     gene            complement(1981..2742)
                     /locus_tag="VFB37_05110"
     CDS             complement(1981..2742)
                     /locus_tag="VFB37_05110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO21864.1"
                     /translation="MPSPQPTPQPPHPPLTTYYEREADRRAWVLDIFERSSADYDRVE
                     RLMALGTGSWYRRRALRRAGLRPGMTVLDIGSGTGLVACEAARIVGDATRVRGVDPCS
                     GMMDQAKVPAGVRLLTGTAEAIPAEDGCADFLSMGYALRHVGDLPVAFAEFHRVLRPG
                     GRLCLLEITRPESAWYRALLKTYMRRVVPFAAGLVARTRATPTLMRYYWDTIELCVPP
                     QSITAALEAAGFMDIDRYVELGIFSEYRATKPAIS"
     gene            2973..3749
                     /locus_tag="VFB37_05115"
     CDS             2973..3749
                     /locus_tag="VFB37_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO21865.1"
                     /translation="MPGNFILETCGLTKEFSGFVAVNGVDLRVCTGHVHALIGPNGAG
                     KTTFFNLLTKFLVPTRGQILYKGNDITSEKASAVARRGLVRSFQMSAVFPHLTVLENV
                     RVALQRSLRTSFHFWKPESSLRVLDRRAGELLESVDLGGFSHRVAVELPYGRKRALEL
                     ATTLAMEPELMLLDEPTQGMGHEDVDKVTSLIKKAAANRTVLLVEHNMKVIATIADQI
                     TVLQHGRIIAAGTYAEVSRNSQVIEAYMGSSEQQLQGARA"
     gene            3746..4450
                     /locus_tag="VFB37_05120"
     CDS             3746..4450
                     /locus_tag="VFB37_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO21866.1"
                     /translation="MSTELLRVAALHAFYGESHILHGLDFSVHEAEIVTLLGRNGAGR
                     TTTFRALLGLVGRRTGSVLIGGREAVNLPPHEIAQLGVGYCPEERGVYSSLTTEENLL
                     LPPVVAPGGMSVDEIYGMFPNLRTRRATQGTRLSGGEQQMLAVARILRTGARLLLLDE
                     ISEGLAPVIVQALSRTIQALRERGYTIVMAEQNFRFAAPLADRFYVMEHGRIVQSFAA
                     SELEGKMVMLHEVLGV"
     gene            4491..5681
                     /locus_tag="VFB37_05125"
     CDS             4491..5681
                     /locus_tag="VFB37_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO21867.1"
                     /translation="MWAIGALVALSAATNSRVAAAADQIVIGDLDDFSGVYADVAGAG
                     AVEATRMAIADFGGSVLGRKIVLLSADHQNKPDIGASKFREWADQNGLNMLLGGSNTG
                     VSIAMAKVAAAKQIPFFAIGAGGASLTNEDCTAYTVHYAYDTTALANGTATTIVKNGG
                     KTWFYLTADYAFGTQLQTAASKVVEANGGKNVGAVRVPLGAADFSSFLLQAQGAGAQV
                     LGLANAGTDFSNSLKAANEFGITRKMKPAALLAFITDINSLGLQTAQGLYLTTGWYWD
                     LNDQTRSFGKRYFEKIKRMPTMDQSGYYSATLTYLKAVKAIGTTDPAKVMEELRKTKI
                     NDIFTKDGHIRADGVMVHDMFVMQVKSPKESKYPWDYYRLVKQMPGAEAFGPITGLCQ
                     LAKK"
     gene            5756..6640
                     /locus_tag="VFB37_05130"
     CDS             5756..6640
                     /locus_tag="VFB37_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZO21868.1"
                     /translation="MTEIFGVPVAVILGQLMLGLVNGSFYALLSLGLAVIFGLMGVVN
                     FAHGAFYALGAFAALLGLQWFGINYWAALLLAPLAVGLLGILCERLFLRRLYGLDPLY
                     GLLLTFGLALVIEGCLHEKFGASGQSYPVPPALEGALNLGFMILPEYRGWVIIVSLLV
                     CFGTWFIIERTRLGATLRAATENAPLLQAFGVNVPLLVALTYGGGVALAALAGVLSAP
                     IILVSAHMGSSLVIVVFAVVVIGGMGSILGSIVSGLLLGVAEGFTKVIYPEASNVIVF
                     VIMALVLILRPAGLFGKD"
     gene            6644..7591
                     /locus_tag="VFB37_05135"
     CDS             6644..7591
                     /locus_tag="VFB37_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZO21869.1"
                     /translation="MRAAPAFYCLAVLFALCAPFLGVYPVFMMKLLCFAMFACAYNLL
                     LGFSGMLSFGHAAFFGSAAYATAWLVAAHGWSTVPAILCGVLVSGFLGLIIGAIAIRR
                     HGIYFAMITLALAQLVYFVCLEAPFTGGENGLQGVPRGTLLGILPLRSDITMYYFVLA
                     AFLAVFIFIRRVIHSPFGQVLKAIRDNEPRALSLGYEIERYKLLAFVLSASLAGLAGS
                     LKALVLGFVTLSDVLQANSGEVILMTLVGGTGTFVGPVVGAAIVVTLQEYLSDIVGGW
                     VTVIIGAVFVICVLVFRRGIVGELAALRRLSGGSSPDRA"
     gene            complement(7652..>8976)
                     /locus_tag="VFB37_05140"
     CDS             complement(7652..>8976)
                     /locus_tag="VFB37_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZO21870.1"
                     /translation="SVASLFTIWFGSNMQLTTIVTGALATILGLSLQRALIALFLGNA
                     FGAIFMALHSAQGPKLGIPQMIQSRAQFGFYGACVPIALVVLMYLGYFASSAVLGGET
                     LAAAWGLGFARSTLILAAITAVLSIYGYRAIHRLERLFSAVAAIAFLYLSARLLGAHD
                     LGALWTGGNTSFGLFLLVVSIAATWQITYAPYVADYSRYLPESTSLRAAFWWTYTGSV
                     ISCVWMMWLGAIAAAVAPQGFSHDSSAFLLKLAAPQVRWAIMLIIATGIVAANVLNLY
                     GMFMSITTTISAMRPISISARARAVAIAVIAVLGTCVAILGQGNFVTNFENFILFLAY
                     FLIPWTAINLADFYLVRKERYDIASIFDPNGIYGRINWRTLCAYTVAIVAEIPFVKTS
                     FYTGRAVERVGGADIAWIIGLVIATLLYLLLMRGEVRTTQPLLDSGKS"
BASE COUNT         1643 a         2869 c         2885 g         1579 t
ORIGIN      
        1 agctgcaggg caacattcgc gcgcagcttg cagatagcct cgtcggtgtg ctctgccagc
       61 gcctggactt tctcgaagcg caccatctgc tcgtgcctcg ttgcgagttg ctgctggcaa
      121 gctccgcagc gcgcggcaca atccgcgcgg gccagttcag caacatcgcc aacgtgattc
      181 agtccggcgg cgaggagggc atgtggacct tcgagcgcta tcagcgatgg atgcagcaga
      241 acaaggcgtg gacacgcgcc gcgccctctg ctgccaccgc cgaggaacgg cccactgcac
      301 cggagcccat cgctgcacct gcgccgctcg gcgcggcacc gggggagatc acggcaccgg
      361 gggaaatcac catcgaagag gacatggatc tggaggagct ggccgagctc gcccggcaga
      421 tcgagcagcg cacgtcctag ctattcgtag ctactcgcct gcggcagcct attcgccctc
      481 ggcagcgctc atcggcgctc ccgcggcggg aacccgctcg ccctggcggc gcgggagggg
      541 tggctcagcc ggaatgagga tgaccgcgag tgcaccggca gccagcgcgc agatcagacc
      601 cgcagcgaag gcaacgtgat agccacttga cagagccgtc acccgcaccg agccctctct
      661 caacatcgcg ccggtgcgtg ccgcggcgag gctcgccagg actgccagcc cgagcgcacc
      721 ccccatcatg aacgaggtgt tgacgagccc ggaggccagt cctgagtccc gcgccggcac
      781 accgctcatg gcagcgagca gcagcgggtt gaaggccatg ccggcgccca cgcccagcag
      841 catcatgccg ggaaccacat cgggcaggat actgccgcct accggagcac gtgcgaagca
      901 ggcaaggccg gccgccgcga acagcaaccc tgcagcgagc gggcgcttga ccccgaagcg
      961 gacgacgagc tttgccgaga gtccgaggga gaacactgcc atgatgaggt tggcgggcag
     1021 gaaggcgaag ccgacctgca tcggacctgc tccgagaacg agctgcagat acaacgtcga
     1081 gatgaaaaac caggcgaaca tcgccgccgc ccagagcgtg gcggcaatgt tggcgcctac
     1141 gagactgcgc agacgaaaca ggcccagcgg catgagcggc gcgcgggcgc gcgattcgat
     1201 cagcagaaag ctggcaaaca gtaccaccgc gcaggcgagc agcccgagcg tacgcgcgga
     1261 agtccaccct gtctcattgc cattgaccac ggcgtagacc gcaagggtta acgatgtagt
     1321 gaccgtcgtc gcccccgcga tgtccagagt cgggctggag tccggtctgc tgcccttgcc
     1381 gagcagcctg ggcacccaca cacagaccac gattccgatg ggcacattga tgaggaacac
     1441 ccagtgccag ttgagtgagc tggtcagcac gccgccgagc agcaggccca ggcttccgcc
     1501 agcggagcag acgaagccgt agatacccat cgccttcgct cgctcctcgt gctcgacgaa
     1561 caggttcatg atcagcgaca gcgcaacggc cgacacgact gcgcctgcca caccctggat
     1621 ggctcttgcc acgatcagca caccctggga gtcggcgagt ccgcagccga gcgaggccag
     1681 cgtgaacagg gccacaccgg acaggaacag gcgacgatga ccgaagagat cgccgagcct
     1741 gccgccgagc agcaggaaac cgccgaacgt gagcaggtag gcattcatga cccagacgag
     1801 ggaggtctcg gaaaagtgca gctgcgctcg gatggacggc agtgcgacgt tcaccactgt
     1861 ggtgtccaga acgatcatca gaacgcccat acacagcacg agcagcgccc accagcgcgt
     1921 acggtcgccc tttccagact gcatggccga ttcactgctc atccaacacc cgtgccgccg
     1981 tcagctgatc gcgggcttgg tggcgcgata ctcggagaag attccgagct ccacgtatcg
     2041 atcgatgtcc atgaagcccg ccgcctcgag tgccgctgtg atggattgcg gcgggacgca
     2101 gagctcgatg gtgtcccagt agtagcgcat cagtgttggt gtggcgcgcg ttcgcgcgac
     2161 caaccctgcg gcaaagggca cgacacggcg catgtaagtt ttgagcaggg cgcgatacca
     2221 ggcactctcg ggacgggtga tctcgagcag acacaggcgc ccgcccggtc gcaaaacccg
     2281 gtggaactct gcgaaggcga cgggcagatc gccgacgtgc cgcagcgcgt atcccatgct
     2341 caggaaatcc gcgcagccgt cctccgctgg gatcgcctcc gccgtgccgg tcagcagcct
     2401 gacccccgcg ggcaccttcg cctggtccat cattcccgag caagggtcca cgccacggac
     2461 gcgcgtggca tcacccacga ttcgtgcggc ctcgcacgcc acgagcccgg ttccggaacc
     2521 gatgtcgagt accgtcatac ccggccgcag gccggcgcgt cgcaaagcgc gacggcgata
     2581 ccaggagccc gtacccagcg ccatgagccg ctcgacccga tcgtagtctg cggatgagcg
     2641 ttcgaagata tcgagcaccc acgcccgccg gtccgcttca cgctcgtaat aggtcgtcaa
     2701 cggcgggtga ggcggttggg gggtaggctg aggagacggc atcggcccga gagcctacta
     2761 ctttgtccgg cacggcataa gccgaaggct taagctttgc ctcgaaacgc gccggttccc
     2821 cccgcgcttg ccggaatcga gtgctgcggc tggccgacgc caatgcgcct ccgtcagcgg
     2881 ccgctgtgcg gcagggttcg tcccgcgagt gcgatgactg ccgcataatg gctgcaaaac
     2941 aaggccctcg ccgcctgacg cttccactga gcatgcccgg taacttcatc ctcgagacct
     3001 gcggcctgac caaggaattc agcggcttcg tcgcggtgaa cggggtggac ctgcgtgtgt
     3061 gcaccggcca tgtccatgcg ctgatcggcc caaatggcgc gggcaagacc accttcttca
     3121 acctgctcac gaaatttctc gtgccaaccc gtgggcagat cctctacaag ggaaacgaca
     3181 tcacttccga gaaggcctcc gccgtggcac gccgcggcct cgtgcgctca tttcagatgt
     3241 cagcggtctt cccgcacctc accgtgctcg agaacgtgcg ggtggctctg cagcgctcgc
     3301 ttcgcaccag cttccacttc tggaaacccg aatcgagcct cagggtcctc gaccggcgag
     3361 ccggcgagct gctcgaatcg gtggatctcg gcggcttctc gcaccgggtg gctgtggagc
     3421 tgccgtacgg gcgcaagcgg gcgctggagc tcgcgacgac cctggcgatg gagcccgagc
     3481 tcatgctgct cgatgagccg acccagggca tgggtcacga agacgtcgac aaggtgacca
     3541 gtctgatcaa gaaggccgcc gccaaccgta cggtcctgct cgtcgaacac aacatgaagg
     3601 tgattgccac gatcgctgat cagatcacgg tgctgcagca cggccggatc atcgccgccg
     3661 gtacatatgc cgaggtctcc cggaattcgc aggtgatcga ggcctacatg ggctcctcgg
     3721 agcagcaatt gcagggcgct cgcgcatgag caccgagctg ctgcgggtag ccgctctcca
     3781 tgcgttctac ggagaatccc acattcttca cggcctggac ttcagcgtcc acgaggctga
     3841 gatcgtcaca ctcctcggtc gtaatggcgc cgggcgcacc acgacgttca gagcgctgct
     3901 cggcctggtt ggccgccgaa ccggctcggt gttgatcggc gggcgggagg ccgtcaacct
     3961 gccgccgcat gagatcgcgc agctgggcgt cgggtactgt cccgaggagc gcggggtgta
     4021 ctcgagcctg acgaccgagg aaaatctgct gcttccgccg gtggtcgcgc ccgggggcat
     4081 gtctgtcgat gaaatctatg ggatgttccc aaacttgcgg acgcggcgag ccactcaggg
     4141 gacgcgtctg tcgggcggag aacagcagat gctggcggta gcgcgcatcc tgcgcacggg
     4201 cgcccggctt ctgctgctgg atgagatctc cgaggggctc gctccggtca tcgtgcaagc
     4261 tctgtccaga accatccagg cgctgcgcga gcgaggctac accatcgtga tggcggagca
     4321 gaacttccgc ttcgctgcgc cgcttgcgga ccgcttctac gtcatggagc atggtcggat
     4381 cgtgcagagc ttcgcggcgt cggagttgga ggggaagatg gtcatgctgc acgaagttct
     4441 gggcgtctga ttccaacaaa ccgatggatg ggagagctat gaaaccgggc atgtgggcaa
     4501 ttggggcctt ggtggcgctc agcgcggcca cgaattcgcg ggtcgctgcc gcagcagacc
     4561 agatcgtgat aggcgacctc gatgacttct ccggggtgta cgccgatgtg gccggcgcgg
     4621 gcgcggtgga agctacccgg atggccattg cggatttcgg cggctcggtt ctcggccgca
     4681 agatcgttct actcagcgcc gaccaccaga acaagccgga tatcggcgcc tcgaaattcc
     4741 gggaatgggc ggaccagaac ggtctcaaca tgctcctcgg gggctccaac acgggagtca
     4801 gtatcgcgat ggccaaggta gccgccgcca agcagatacc gttcttcgcc atcggcgcag
     4861 gtggcgcctc gctcaccaat gaggactgca ccgcgtacac ggtgcattac gcctacgaca
     4921 ccactgcgct tgcgaatggg acggccacga ccatcgtcaa gaacggcggc aagacctggt
     4981 tctatctgac tgccgactat gcattcggca ctcaattgca gaccgcggca tcgaaggtcg
     5041 tcgaagccaa cggcggaaag aatgttggtg cggttcgcgt gccgcttgga gccgcagact
     5101 tctcctcgtt cctgctgcag gcacaggggg cgggcgcgca ggtgctcggc ctggccaacg
     5161 ccggtacgga cttcagcaat tcgctcaagg ccgccaacga gttcggcatc acgcgcaaga
     5221 tgaagcccgc tgcgctgctt gcgttcatca ccgacatcaa cagcctgggc ctgcagaccg
     5281 ctcagggtct gtatctgacg acgggctggt actgggacct gaatgatcag actcgctcct
     5341 tcggcaagcg ctatttcgag aagatcaagc gcatgcccac catggatcag tccggctact
     5401 actccgccac gctcacctat ctgaaggcgg tcaaggccat cggcaccacg gacccggcga
     5461 aggtcatgga ggagctgcgc aagacgaaga tcaatgacat cttcaccaag gatggccaca
     5521 tccgcgcgga tggtgtgatg gtccacgata tgttcgtgat gcaggtgaag tcgcccaagg
     5581 aatccaagta cccgtgggac tactaccggt tggtgaagca gatgcccggc gcagaggcat
     5641 ttggtcccat caccggcctg tgtcagctcg cgaagaagta agtgcgcaag gctctgaccc
     5701 acgctgcagg cagcctggca ccccgtgagg cccccttcct gctggcgcca gcgccttgac
     5761 tgagatcttc ggcgtaccgg tcgccgtcat cctcgggcag ctcatgctcg gcctggtgaa
     5821 cggctcgttc tatgcgctgc tcagtctcgg gcttgcggtg atattcgggc tgatgggcgt
     5881 ggtcaatttc gcgcacggcg ccttctatgc gctgggggcg tttgccgccc ttctcggcct
     5941 gcagtggttc ggcatcaact actgggctgc cttgctgctg gctccgctgg ctgtcgggct
     6001 gctgggcatt ctctgcgagc gattgttcct gcgacggctg tacggcctcg atccgctgta
     6061 tggtctgctc ctgacctttg gtctggcgct cgtgatcgag ggttgtctgc acgagaagtt
     6121 cggcgcctcc gggcagagct atcccgtccc accggcactc gaaggcgccc tcaatctggg
     6181 gttcatgatc ctgcccgaat accggggctg ggtaatcatc gtctcgctgc tcgtgtgctt
     6241 cggcacctgg ttcatcatcg agcgcacccg tctgggcgcc accttgcgcg ccgcgacgga
     6301 aaatgcaccg ctgctacagg ccttcggtgt caacgtcccc ctcctggttg ctctcaccta
     6361 cggaggtggc gtcgcgttgg cggctctggc aggggtgctg tcggcgccga tcatcctcgt
     6421 gagcgcacac atgggcagct cattggtgat cgtcgtcttt gcggtggtgg tgatcggcgg
     6481 tatgggctcg atcctcggct cgatcgtcag cggtctgctc ctcggggtcg cagagggctt
     6541 cacgaaggtg atctatcccg aggcttccaa cgtcatcgtg ttcgtgatca tggcgctcgt
     6601 gctcatcctg cgtcccgcgg gcctgttcgg gaaggattag cacatgcgcg ccgcgccagc
     6661 tttctattgc ctcgccgtac tgttcgcgct ttgcgccccg ttcctggggg tctacccggt
     6721 cttcatgatg aagctgctgt gcttcgcgat gttcgcgtgc gcctacaacc tgctgctggg
     6781 cttcagcggg atgctctcct tcgggcacgc cgccttcttc ggctcggcgg cctacgccac
     6841 ggcatggctc gtggcggcgc acgggtggtc caccgtgcca gcgatactct gcggcgttct
     6901 ggtgtcggga tttctggggc tgatcatcgg cgcaatcgcc atccgccggc atggcattta
     6961 cttcgccatg atcacgctcg cactcgcaca gctggtgtac ttcgtgtgtc tcgaggcgcc
     7021 ctttaccggc ggcgagaacg ggctgcaggg ggtgccccgc ggaacgcttc tcggaatcct
     7081 gcccctgcgc tccgatatca cgatgtatta cttcgtgctg gcggccttcc tggcggtatt
     7141 catcttcatc cgccgcgtga ttcattcccc cttcggccag gtcctcaagg ctatccgcga
     7201 taacgagccg cgcgccctgt cgctgggtta cgagatcgag cgctacaagc tgctcgcctt
     7261 cgtactctcg gcgagcctcg cgggcctcgc cggttcgctc aaggctctgg tactcggctt
     7321 cgtgacgctt tcggacgtgc tgcaggcaaa ctcgggtgaa gtgatcctga tgaccctggt
     7381 cggcggaaca ggcactttcg tgggccccgt ggtaggcgcc gccatcgtgg tcacgctgca
     7441 ggaatacctc tccgacatcg tgggcggctg ggtgacggtg atcatcgggg ccgtgttcgt
     7501 catctgcgtc ctggtgtttc gccgtggcat agtgggcgag ctcgccgcct tgcgcagact
     7561 ctcaggcggt agcagccccg atcgcgcgtg atcgtcaacc cccatggcct gtctaggcgg
     7621 tggcgcggcc gcaccgcagg tcttgctcgg ctcagctctt gccgctatcc agcagtggct
     7681 gcgtcgtgcg tacttcgccg cgcatcagca gcagatataa gagcgtggcg atcaccaggc
     7741 cgatgatcca ggcaatgtcg gctccaccca cgcgttccac agcacgtcct gtgtaaaagc
     7801 tcgtcttgac gaagggtatt tcggcgacta ttgcgacggt atacgcgcac agcgtgcgcc
     7861 agttgatccg tccatagatg ccgttcggat cgaagatcga ggcgatgtcg taacgctcct
     7921 tgcgcaccag atagaaatcc gccaggttga tggccgtcca gggaatgagg aaataggcca
     7981 ggaacaggat gaagttctcg aagttggtga cgaagtttcc ctggccgaga atcgccacgc
     8041 aggtgcccag aacggcaatc accgcgatcg ccacggcgcg tgcgcgcgcg ctgatgctga
     8101 tcggtcgcat cgcgctgatc gtggtcgtga tcgacatgaa catgccatag aggttgagca
     8161 cgttggctgc cacgattccc gtcgcgatga tgagcatgat ggcccagcga acctgcggcg
     8221 cggcgagctt gaggaggaat gccgaggaat cgtgactgaa gccttggggc gcgacggcgg
     8281 ccgcgattgc ccccaaccac atcatccaga cgcacgagat gacactgccg gtataagtcc
     8341 accagaaggc ggcgcgcagc gaggtactct cgggcaggta gcgggagtag tcggcgacat
     8401 agggtgcgta ggtgatctgc caggtcgcgg caatcgatac caccagcaga aagaggccga
     8461 aggatgtatt gccccccgtc cagagagcac ccaggtcatg tgcgccgagc aggcgggcgg
     8521 agagatagag gaaggcgatc gcggccaccg cgctgaacag acgctccagc cgatgaatcg
     8581 cccggtagcc atagatgctc agcacagcgg taatggcggc gagtatgagt gtcgagcgcg
     8641 cgaagcccag accccacgcc gccgcgagcg tctcgcctcc gagcaccgcg ctgctggcga
     8701 aataacccag atacatcagc accacgagcg caatcggcac acacgctccg tagaaaccga
     8761 actgagcgcg gctctggatc atctgcggga tcccgagctt ggggccttgc gccgagtgca
     8821 gcgccatgaa aatggccccg aacgcattgc cgagaaacag ggcgatcagc gcgcgctgca
     8881 gagaaagtcc gaggatcgtg gccagtgctc ccgtgacgat ggtggtgagc tgcatgttgc
     8941 tgccgaacca gatcgtgaac agactggcca cggagc
//
LOCUS       DASRHN010000135         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1364412, whole genome shotgun sequence.
ACCESSION   DASRHN010000135 DASRHN010000000
VERSION     DASRHN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364412"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFB37_05145"
     CDS             <1..372
                     /locus_tag="VFB37_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZO21871.1"
                     /translation="VDTQSTAIQDVTDKPVQKPPPPAPKAVNRVQGGPGKGFPNSDDY
                     YPAASRRLNEQGAAVVHVCIDAKGKVTAEPTVQQTSGSARLDEGAVKLAKAGSGRYRA
                     TTENGTPVDSCFPMSIRFQLH"
     gene            428..1171
                     /locus_tag="VFB37_05150"
     CDS             428..1171
                     /locus_tag="VFB37_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZO21872.1"
                     /translation="MQIQTTATADNPYGLAALWASGDIIARAVLVLLCIMSLASWYVI
                     FTKLWDQRKLKQSARVAEKQFWTAPSIKDGVERLKKGDDFRLVAEDGLRAASHHDGRL
                     TDRIDLHEWITMSLQRAVDGVNTRLNSGLGLLATVGSVAPFVGLFGTVWGILNALVSI
                     GIAGQASIDKVAGPVGEALIMTAIGLFVAVPAVMGYNWLLGRNKLIQEALRNFASDLH
                     AYLVSGARVGSGEVEARPATAAPAAALRK"
     gene            1228..1671
                     /locus_tag="VFB37_05155"
     CDS             1228..1671
                     /locus_tag="VFB37_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZO21873.1"
                     /translation="MSMNVGGSSEEKEVMSVINTTPLVDVMLVLLIIFLITIPVINKT
                     VKVDLPKAVNIPTQTKPENITIAVDKDGNIYWNDRRMPNRDELLAHIKEAAVRKPQPE
                     IHIRGDKDARFEAIGRVLYAIQRGGVVKVGFLTEPDHGVRGTRLP"
     gene            1748..2170
                     /locus_tag="VFB37_05160"
     CDS             1748..2170
                     /locus_tag="VFB37_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZO21874.1"
                     /translation="MAMSVGTAEGAAMCDINTTPLIDVMLVLLVTLIVSLPIMTHAVK
                     LDMPQANQPPPPDARPEVIDLEIDFDGTVVWNGTVVQSLNQLEGYFHEEAQKQPQPEI
                     HLRPDRRAKYDYVAKVLASAQRNRMKKIGFVNTSEFRD"
     gene            2208..3461
                     /locus_tag="VFB37_05165"
     CDS             2208..3461
                     /locus_tag="VFB37_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO21875.1"
                     /translation="MQAPGKAVAAFFLALALIGAGGLLASRSAAAADEKKKDTVSREL
                     AKPLKAAQDDIQAKKYSDALAKIKEADGNPKKTPFDEHVINQLAGFAYAKTNNYPEAA
                     KAFEAQINDGFTSPEEVERLTKAVAQIYYQLKSYDKAIEYGNRLIKSGQADDEMYTLV
                     GQSYYLKNDYKGTLKFEDNFVDAQIKKGQQPKKEPLELALSSCVKMNDSDCTSHELER
                     LVAYYPKPEYWAQLLDTLLRSPQKNDKVQLQVYRLMSEVDVLKEPGDFTDMAQIALDQ
                     GSPGEAQHTLEKAFQKNVFPDKRTQDKNQRLLESAKKAAANDLATLPKAAKDAESAPT
                     GDKEVGVGLAYLGYQQYDKAVDALSKGIAKGGLKSEPEARLLLGIAQLKAGHKDDAVK
                     AFHAVKGDPTLERLATLWTLHAKQA"
     gene            3660..4142
                     /locus_tag="VFB37_05170"
     CDS             3660..4142
                     /locus_tag="VFB37_05170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO21876.1"
                     /translation="MAIKAGERMPSGKLKTMTKEGPKDVTTDELFKGKKVVLFSVPGA
                     FTPTCDAKHLPGFVQVAEQIHAKGVDTIACMAVNDVFVMNAWGKASSVGDKILMLADG
                     NGEYAKALGLELDAKGYGMGMRGQRFAIVVQDGTAKHVEIEQPGQFKVSAAEHVLSKL
                     "
     gene            4166..>4814
                     /locus_tag="VFB37_05175"
     CDS             4166..>4814
                     /locus_tag="VFB37_05175"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZO21877.1"
                     /translation="MHDAGDRNQRIVLSRRPVGAPLPENFGADTTAVPTPGPGQFLSR
                     TVYLSLDPSYRPTLHEGAQSAVRLNLGDVMSGETVAQVLESRHPDYRQGEYVAVRNGW
                     QQFALSSGQGVRRLDPTRAPVSTALGVMGLPGLAGYTGLVYFGEPRPGQTVLVSAATG
                     PVGCTAGQAARIIGARPVGIAGSAQKCDYAVRELGYAACLNYRAADLATQIERECP"
BASE COUNT          974 a         1516 c         1559 g          765 t
ORIGIN      
        1 gttgacacgc agtccacggc gatccaggac gtcactgaca agccagtgca gaagccgccg
       61 ccaccggcgc cgaaagcggt caaccgtgtg cagggtggcc cgggcaaggg cttcccgaac
      121 agcgacgact actacccggc agcctcgcgc cgcctgaatg agcagggcgc agcagtggtg
      181 cacgtctgca tcgacgccaa aggcaaagtc accgcggagc cgacggtcca acaaacttcc
      241 ggcagcgccc ggctcgatga aggagcagtc aagctcgcca aggccggctc aggccgctat
      301 cgcgccacca ccgagaacgg cacgccggtc gattcgtgct tcccgatgtc cattcgcttt
      361 cagttgcact gaagaaaaat gttcgtgccg ggccatagcc cggctttttt tgtgatccga
      421 ggaaactatg caaattcaaa ccaccgcaac cgccgataat ccttacggcc tcgccgctct
      481 gtgggcctcg ggcgacatca tcgcccgcgc cgtcctggtc ctgctgtgca tcatgtcgct
      541 ggcctcctgg tatgtgatct tcaccaagct gtgggatcag cgaaagctca agcagtcggc
      601 cagggtggcc gagaagcagt tctggacggc cccctcgatc aaggacggcg tcgagcggct
      661 gaagaagggc gatgacttcc ggttggtcgc tgaggacgga ctgcgcgccg cctcccatca
      721 cgacggccgc ctcacggacc ggatcgatct gcacgaatgg atcaccatgt cgctgcagcg
      781 tgccgtggac ggggtgaaca cccggctcaa ttccgggctg ggactgctcg ccaccgtcgg
      841 ctccgtcgcc cccttcgtcg gcctgttcgg cacggtgtgg ggcatcctga acgcgctcgt
      901 gtcgatcggt atcgccggcc aggcaagcat cgacaaggtg gcaggccccg tcggtgaggc
      961 cctcatcatg acggccatcg gtctgttcgt cgccgttccg gcggtgatgg gctacaactg
     1021 gctgctcggc cgcaacaagc tgatccagga agcgttgcgg aacttcgcga gcgacctgca
     1081 tgcctacctg gtaagcggtg cccgcgtagg cagcggcgag gtcgaagccc gtccggccac
     1141 ggcagctccg gcagcggcgc tgcgcaagta gtcatgacgg cacgcggcgg tgccccggcg
     1201 gccgccgcgt agcgcgagga tttgcatatg tcgatgaatg tcggcggctc gagcgaagag
     1261 aaagaggtga tgtcggtcat caacacgacg ccgttggtgg acgtcatgct ggtgttgctg
     1321 atcatcttcc tgatcaccat cccggtcatc aacaagaccg tgaaggtcga tctgccgaaa
     1381 gcggtgaaca ttccgactca gaccaagccg gagaacatca ccatcgcggt ggacaaggac
     1441 ggcaacatct actggaacga ccgcaggatg cccaaccgcg atgagctgct ggcgcacatc
     1501 aaggaggccg cggtgcgcaa gccccagccc gagatccata tccgcggcga caaggatgcg
     1561 cgcttcgagg cgatcggccg ggttctgtac gcgatccagc gcggcggcgt ggtcaaggtc
     1621 ggattcctga ccgaacccga ccatggcgtg cgcggtactc gcttaccatg acctgttcga
     1681 agaaggcatc gtcagccggg ccgaccgtga caggcgcacg gccggacaac acaggagtcg
     1741 cagcaagatg gcaatgagtg taggcaccgc tgaaggtgcc gcgatgtgcg atatcaacac
     1801 gacgccgctg atcgacgtca tgttggtgct tctggttacg ctgatcgtct cgctgccgat
     1861 catgacgcac gcagtgaagc tggacatgcc gcaggcgaat cagcccccac cgcccgatgc
     1921 gcgacccgag gtcatcgatc tggagatcga cttcgacggc acggtcgtct ggaacggcac
     1981 cgtggtgcag agcctgaacc agctcgaggg ctatttccac gaggaggcgc agaagcagcc
     2041 gcagcccgag atccatctgc ggcccgaccg ccgcgccaaa tacgattacg tagcgaaggt
     2101 gctggcctcg gcccagcgca atcgcatgaa gaagatcgga ttcgtcaaca cctcggagtt
     2161 ccgcgattaa cccccgcgta ccaacaccta cgatgcacaa tcacacgatg caagcccccg
     2221 gcaaggctgt tgccgccttc ttcctcgcgc tcgccctcat cggcgcgggc ggactgctgg
     2281 cgagccgctc ggccgctgca gcggatgaga agaagaagga caccgtcagc cgggagctcg
     2341 ccaagccgct gaaggcggcc caggatgata tccaggcgaa gaagtactcg gatgcactcg
     2401 ccaagatcaa ggaggcggac ggcaacccga agaagacgcc cttcgacgag cacgtcatca
     2461 accagctcgc gggctttgcc tacgccaaga ccaacaacta tcccgaagcg gccaaggcct
     2521 tcgaggcgca gatcaacgat ggcttcacga gccccgagga ggtggagcgt ctgacgaagg
     2581 cggtcgcgca gatctactat cagctcaaga gctacgacaa ggccatcgaa tacggcaacc
     2641 ggctcatcaa gtccgggcag gccgacgatg agatgtacac gctcgtgggc cagtcctact
     2701 acctcaagaa cgactacaaa ggcacattga agttcgagga caacttcgtc gacgcccaga
     2761 tcaagaaagg ccagcagccg aagaaggagc cgctcgagct ggccctgagc tcatgcgtca
     2821 agatgaacga cagcgactgc acgagccacg agctcgagcg gctggtcgcc tattacccga
     2881 agccggagta ctgggcgcag ctgctcgata cgctgctgcg ctccccgcag aagaatgaca
     2941 aggttcagct gcaggtctac cgcctcatgt ccgaggtgga cgtcctcaag gagcccgggg
     3001 acttcaccga catggcgcag atcgcgctcg atcagggctc gcccggcgaa gcccagcaca
     3061 cgctcgagaa ggcattccag aagaacgttt tccccgacaa gcgtactcag gacaagaatc
     3121 agcgcctgct cgaatccgcc aagaaggccg ccgccaatga cctggcgacg ctgccgaagg
     3181 cagcgaagga tgctgagtcg gccccgaccg gcgacaagga ggtcggagtc ggattggcct
     3241 atctcggcta tcagcagtac gacaaggcgg tcgatgcgct cagcaagggc atcgccaagg
     3301 ggggcctcaa gagcgagccc gaagcgcggc tgctgcttgg gatcgcgcag ctgaaggctg
     3361 gacacaagga tgacgcggtg aaggcgttcc atgcggtgaa aggggatccg acactggaac
     3421 gtctggcgac tctctggacg ctgcacgcca agcaggcttg acgccgacgc gctgaggcgg
     3481 ccccgcttac ccgcggcggt acgcagaggc gagattccac aaggccggaa gcgtgagctt
     3541 ccggcctttt taatatgcac gagcgtgcgc ccgcggaggc aggagccgtt tgagccacta
     3601 aaccttcgcg ccggtggttt acgatatcgc cttcacgagg cgtaattcgg agactcaaga
     3661 tggcgatcaa ggctggcgag cggatgcctt ccggcaagct caagacgatg accaaagaag
     3721 gcccgaagga tgtgacgacc gatgagctct tcaagggcaa gaaggtcgtg ctgttctcgg
     3781 ttccgggagc attcacaccc acgtgcgatg cgaagcacct gcccggtttc gtgcaggtcg
     3841 ccgagcagat ccacgcgaag ggcgtcgaca cgatcgcctg catggcggtg aacgacgtgt
     3901 tcgtgatgaa tgcctggggg aaggcctcca gcgtcggcga caagatcctc atgctcgccg
     3961 atggcaatgg tgagtatgcc aaggcgctcg gactggagct cgatgcgaag ggctacggca
     4021 tgggtatgcg cgggcagcga ttcgccatcg tggtgcagga cgggacggcc aagcacgtcg
     4081 agatcgagca gcccgggcag ttcaaggtct ccgcagcgga gcatgttctg agcaagctgt
     4141 aaggcgcgag gcggcgcgat ctttcatgca cgacgcgggc gaccgcaatc agcgcatcgt
     4201 cctgagcaga cgccccgtcg gcgcgccgct tcctgagaac tttggcgcgg atacgactgc
     4261 ggtgcccacg ccgggccccg gtcaattcct gtcgcgcacc gtgtatctgt cactcgatcc
     4321 gtcctatcgc cccacgctgc acgagggtgc ccagtccgcc gtgcggttga acctcgggga
     4381 cgtgatgagt ggcgagaccg tggcgcaggt gctcgagtcg cgccaccccg actaccggca
     4441 gggcgagtac gtcgccgtgc gcaacggctg gcagcagttc gcgctgtcga gcggccaggg
     4501 cgtgcgacgc ctggatccca cccgggcacc ggtctccacc gcgctcggcg tcatgggttt
     4561 gccgggtctc gccggctaca caggcctggt gtacttcggt gagcctcggc ccgggcagac
     4621 cgtgctcgtg tcggccgcga cggggccggt cgggtgcacg gcgggtcagg ccgcgcgcat
     4681 catcggcgcg cggccggtgg ggatcgccgg ctctgcacag aagtgcgact acgccgttcg
     4741 cgagttggga tacgcggctt gtctgaatta tcgcgcggcc gacctcgcta cacagatcga
     4801 gcgcgagtgt cccg
//
LOCUS       DASRHN010000136        10919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_374536, whole genome shotgun sequence.
ACCESSION   DASRHN010000136 DASRHN010000000
VERSION     DASRHN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374536"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VFB37_05180"
     CDS             <1..1028
                     /locus_tag="VFB37_05180"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HZO21878.1"
                     /translation="AYVKTFSGPAAGIVVERERLEKFGRPLLGATVKPKLGLSGRNYG
                     RVVYEALKGGLDFTKDDENINSQPFMHWRDRFLNCMEAVNRAQAATGEVKGHYLNVTA
                     GTMEEMYERADFARQLGSVVVMIDLVIGYTAIQSMARWARRNDVLLHLHRAGHSTYTR
                     QRNHGISFRVICKWMRLAGVDHIHAGTVVGKLEGDPNSVQGFYNVLRESHNSVDLQRG
                     VFFEQDWAGLPGVMPVASGGIHAGQMHQLLHYLGEDVVLQFGGGTIGHPMGIQAGATA
                     NRVALEAMVLARNEGRDIWNEGPAILTEAARHCSALRAALDVWGEITFNYTSTDTPDF
                     VPTLTSA"
     gene            1040..1489
                     /locus_tag="VFB37_05185"
     CDS             1040..1489
                     /locus_tag="VFB37_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HZO21879.1"
                     /translation="MRLTQGTFSFLPDLSDAQIARQIEYCLHNGWAVSIEHTDDPHPR
                     NTYWEMFGAPMFDIRDAAGIMTEVHSCRRTFPERYIRVNAFDSSLGRESLRLAFIVGR
                     PAQEPGFALERHEGPGRTIQYTTRAYATDRPEGERYRSAGTNAPADE"
     gene            1506..2450
                     /gene="cbbX"
                     /locus_tag="VFB37_05190"
     CDS             1506..2450
                     /gene="cbbX"
                     /locus_tag="VFB37_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HZO21880.1"
                     /translation="MNAPAKAPAEGQTAQLDLDAAFRESQIGEVLEQLDRELVGLAPV
                     KSRIRDIAALLLVDKLRRTLDLSAGAPSLHMSFTGNPGTGKTTVALRMAEILHRLGYV
                     RKGHLVAVTRDDLVGQYIGHTAPRTREVLKRAMGGVLFIDEAYYLYRPENERDYGQEA
                     IEILLQVMENQRADLVVILAGYRNRMDTFFRSNPGMSSRVAHHIDFPDYTPAQLHAIA
                     GLMLEQMQYRLSPQADEALTEYIARRILRPHFANARSVRNALDRARLRQASRLFGRRG
                     GLLSREELLTLEAADIRASRVFAPELDEEAASGSEALW"
     gene            2447..3511
                     /locus_tag="VFB37_05195"
     CDS             2447..3511
                     /locus_tag="VFB37_05195"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006778.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZO21881.1"
                     /translation="MKALTLTRFLIEEQRRYPAATGGFTALVNDVRLACKRIATVIGR
                     GALEETLGAAGSTNVQGEEQMKLDVLANDVFVRTNEWGGQLAGMLSEEMTDPYLIPEE
                     YPRGRYLLLFDPLDGSSNTDVNVSVGSIFSVLRCPEGVSEPVESDFLQPGTRQVCAGY
                     AIYGPATMLVLTTGRGVNGFTLDREIGEFMLTHPDLRIDPDTSEFAINASNSRYWEPA
                     IKRYVKECLAGRSGPRGRDFNMRWIASLVAETHRILMRGGVFMYPRDRKDLTRAGRLR
                     LLYEANPIGLLIEQAGGRASTGQRPILEVEPQSAHQRIGFVFGARHEVERIERYHRDH
                     NEFEFEAPLFGTRGLFTTRI"
     gene            3528..4430
                     /locus_tag="VFB37_05200"
     CDS             3528..4430
                     /locus_tag="VFB37_05200"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HZO21882.1"
                     /translation="MSARHPIIAVTGSSGAGTTSVMRTFEHIFRREKIRAGFIEGDSF
                     HRYDRAEMKQRLQSALDRGDHRFSHFGPDANLFAELEALFRDYGHRGRGRFRRYLHDE
                     AEASRHAQPPGTFTEWEELTSDTDVLFYEGLHGAIVTEEVNVARHVDLLIGVTPVINL
                     EWIQKLHRDKATRGYSAEAVTDVILRRMHDYVQYICPQFSRTHINFQRVPVVDTSNPF
                     IARFIPTPDESFLVIHFSNPGGIDFPYLLSMLHDSFMSRANTIVCPGGKMDLAMQLIF
                     TPMIWRLMECRRESLQRGAMAACG"
     gene            4427..5224
                     /locus_tag="VFB37_05205"
     CDS             4427..5224
                     /locus_tag="VFB37_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZO21883.1"
                     /translation="MRGLQALIFDVDGTLADTEELHREAFNTAFRSFDLPWNWDAALY
                     GRLLQVTGGKERIARYIDELPILESERARLKGRVPDLHAAKTRFYRQLIGLGRVRPRA
                     GVRRLMLEALEAGVRLGIASTTSPENVEPLIRAAFGQATLSWFATIATGDIVQHKKPA
                     PDIYNVAVAELTVPASGAVAVEDSAIGVESAKRAGLFAVAVPCRWTRTQDFSSADLVL
                     DSLGEPDHPLDEASARRLGARYLSLEHLAALHGASLSDRRRGDGRST"
     gene            5208..5954
                     /locus_tag="VFB37_05210"
     CDS             5208..5954
                     /locus_tag="VFB37_05210"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HZO21884.1"
                     /translation="MADPPESFRAAAFDLDGTLIDTLPDLAAAVNLMLGMLGARELAP
                     SAIRALVGGGVEKLVHRALAESLGEQSAHAAQRSAAVTLFGRLYGQALFERSQLYPGV
                     EAALRALSAAGVGLCCVTNKDSRFATPLLERAGLRRFFAFTLCADTPEERKPSPHLLL
                     SACSRLGIAPAQMVYVGDSRVDIAAARAAGCRVVVVRYGYDTPALLEQAQPDAIVDTL
                     ADLPSLRLSSAPPDATRAPFDQRGSGATPR"
     gene            complement(5905..6597)
                     /locus_tag="VFB37_05215"
     CDS             complement(5905..6597)
                     /locus_tag="VFB37_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HZO21885.1"
                     /translation="MIDLYYWTTPNGHKITMMLEEIGLPYRIVPVEIAKGAQFEPAFL
                     AISPNNRIPAIVDREPAGGGAPISLFESGAILLYLAEKSGRLLPRDVRGRAEVTQWLF
                     WQMAGLGPMAGQNHHFSRYAPEKLPYAIQRYVNETNRLYGVLNQRLAERAFVAGEYSI
                     ADIAAYPWIVPYEAQGQKLEDFPHLKRWFEAIRDRPATIRAYEKAQQINTQPTVSSEE
                     SRQILFGQRARA"
     gene            complement(6764..7420)
                     /locus_tag="VFB37_05220"
     CDS             complement(6764..7420)
                     /locus_tag="VFB37_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZO21886.1"
                     /translation="MSHITQIQQIRDRARKQITEGAVTEDYQLDRQKAIGILNEALAT
                     ELVCVLRYRFHFFMASGIHSQAVKAEFKEHAEEEQEHADWIAERIKQLGGKPDMNPAI
                     LTERSHSEYKEGTSLADMIREDLIAERIAIETYREISKFFGEKDSTSRRLIEQILAKE
                     EEHADDMADLLFAVEPDTGEGARPLYFADEVPGATNELKVSSTQAITKGANKPGAKPS
                     "
     gene            complement(7503..8648)
                     /gene="nagA"
                     /locus_tag="VFB37_05225"
     CDS             complement(7503..8648)
                     /gene="nagA"
                     /locus_tag="VFB37_05225"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HZO21887.1"
                     /translation="MATALINGQVMGDAGLIENHAVMLEGGLITGLVPESDPRCAQLE
                     RRDLGGALLLPGFIDCQVNGGGGVLFNDSPSLEVIRSIGIAHRKFGTTGFLPTLISAD
                     LEVVARAIGAVQAAIKAGVPGVLGIHIEGPFLNVARKGVHDQAKLRELDASGIGLLTS
                     LRGGRTLVTLAPEMTTPELIRTLVSAGVVVSAGHTNATYAEIASALRHGLSGFTHLFN
                     AMSQLTGREPGVVGAALDDQQSWCGIIVDGAHTDPVVLRIALRCKRHDRFMLVTDAMP
                     SVGTDTKSFNLQGRHITVSGSLCVDEDGRLAGSNVDMASCVRNAVSMLGLPLSEAVRM
                     ASLYPAQFLGLSDQVGRIAPGYRANLVLVDEQLQVLDTWIDGIPIAG"
     gene            complement(8648..9697)
                     /locus_tag="VFB37_05230"
     CDS             complement(8648..9697)
                     /locus_tag="VFB37_05230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZO21888.1"
                     /translation="MISPNTTSRPESTSLFREAAQAAAIVRQQFAANESLMGELGARL
                     RGLGPKAVVTCARGSSDHAATFAKYLIETRLGLVTASAAPSVSSLYEAQPQLRDMLLL
                     AISQSGASPDLLAAVDSARAAGSLIVALVNAESSPLARAADIAIPLRAGEERSVAATK
                     SYIASIAAVIHLVAAWAQDGRLLEQLRRAAESLERAWERDWSSALSRLQGADHLFVVG
                     RGLGLGIAQEAALKLKETCGIHAEALSAAELRHGPIALVKPGFPVLIFAQSDETHASV
                     ESLAAEIASRGAEVVIAGARAPQALVLPSEEAPAAIQPMLLIQSFYRLVNSLALARGY
                     DPDRPPHLRKITETV"
     gene            complement(9694..10434)
                     /locus_tag="VFB37_05235"
     CDS             complement(9694..10434)
                     /locus_tag="VFB37_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO21889.1"
                     /translation="MNLLSTVGALDESSSLPLYQQLQRALRQAIERRVLGPDDALPAE
                     RDLATEFSVSRITVRKAIDGLVSEGLLVRRQGSGTFVTARVEKNFSKLTSFSEDMRAR
                     GRTPRSVWLQRSAGTVSPEEALTLRSSPGTPVFRFHRIRFANDSPMSLEYATVLASSL
                     PSLEAVESSLYEALERAGNRPVRALQRLRAVLLTGDQAELLQAKERDAGLLVERLGFL
                     QDGRAAEFTQSYYRGDTYDFVAELSISS"
     gene            10719..>10919
                     /locus_tag="VFB37_05240"
     CDS             10719..>10919
                     /locus_tag="VFB37_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21890.1"
                     /translation="MRIARSTLVGTAVAMALFGRNGAVRADAPDTTSSQNGPLEEVVV
                     HGYRMALRESMETKRAATGVEEV"
BASE COUNT         1941 a         3510 c         3525 g         1943 t
ORIGIN      
        1 cagcctacgt caagaccttc tccgggccgg cggccggcat cgtcgtggag cgcgagcggc
       61 tggagaagtt cggtcgcccc ctgctcggag cgaccgtcaa accgaagctc ggcctctcgg
      121 gacgcaacta cggacgtgtc gtgtacgagg cgctcaaggg cgggctggat ttcaccaagg
      181 acgacgagaa catcaactcg caacccttca tgcactggcg cgatcgcttc ctcaactgca
      241 tggaggcggt caaccgggcg caggcggcga cgggcgaggt caagggtcac tacctgaacg
      301 tcaccgccgg gacgatggag gagatgtacg agcgggctga tttcgcccgc cagctgggct
      361 ccgtggtggt catgatcgat ctggtgatcg gctacacggc tatccagtcg atggcacgct
      421 gggccagacg caacgatgtg ctgttgcacc tgcaccgggc cggtcattcg acctacacgc
      481 gtcagaggaa tcatggcatc tccttccgcg tgatctgcaa gtggatgcga ctggcgggtg
      541 tcgatcacat ccacgccggc acggtcgtgg gcaagctcga aggcgatccc aactcggtgc
      601 aaggcttcta caacgtgctg cgcgaaagcc acaacagcgt cgatctgcag cgcggtgttt
      661 tcttcgagca ggactgggcc ggtttgcccg gggtgatgcc ggtggcctcc ggcggcatac
      721 atgccggcca gatgcaccag cttctgcatt atctgggcga ggacgtcgtc ctgcagttcg
      781 gcggcgggac catcggacat ccgatgggaa tccaggccgg ggcgaccgcc aaccgggtgg
      841 cgctcgaggc catggtgctg gcccgcaacg aggggcgcga tatctggaat gagggcccgg
      901 cgatcctgac ggaggcggcg cgccattgca gtgctctgcg ggcagcgctc gatgtctggg
      961 gcgagatcac cttcaactac accagcaccg acacgccgga ttttgtgccg actttgacga
     1021 gtgcctgagg agcaccagca tgagactcac gcagggcacg ttctcgttcc ttcccgattt
     1081 gagcgatgcg cagatcgcac ggcagatcga gtactgcctg cacaatggct gggccgtctc
     1141 gatcgaacat accgacgatc cccatcctcg caacacgtac tgggagatgt tcggcgcgcc
     1201 catgttcgac atccgcgatg ccgctggaat catgaccgag gtgcacagct gccgcaggac
     1261 cttcccggag cgctacatca gggtcaacgc attcgactcc tcgctcgggc gcgagtcgct
     1321 gcgcctggca ttcatcgtcg gccgtcccgc ccaggagccg ggcttcgcgc tcgaaaggca
     1381 tgaggggccc ggacgtacga tccaatacac cacgcgtgcg tatgccacgg atcggcccga
     1441 aggcgagcgc tatcgctcag ccgggacgaa cgcacccgcc gatgaataaa cccgccggcc
     1501 gataaatgaa cgcgccagcc aaagcgccag ccgaagggca gacggctcag ctcgatctgg
     1561 acgccgcatt tcgcgaatcg cagatcggtg aggtgctgga gcagctcgat cgcgagttgg
     1621 tcggtctcgc gccggtgaag agccggatcc gcgacatcgc cgcgctgctg ctggtggaca
     1681 agctgcggcg cacgctggat ctgagcgccg gggccccatc gctgcacatg agcttcaccg
     1741 gcaatcccgg aaccggcaag accaccgtgg cgctgcgcat ggccgagatc cttcatcgcc
     1801 tgggctacgt gcgcaagggt catctggtcg cggtcacgcg cgatgacctc gtcggtcagt
     1861 acatcggcca cacggcgccc aggacccggg aagtgctcaa gcgtgcgatg ggcggcgtgc
     1921 tgttcatcga tgaggcctac tacctctacc ggccggagaa cgagcgcgat tacggtcagg
     1981 aggcgatcga gatcctgctg caggtcatgg agaatcagcg cgccgatctg gtcgtcattc
     2041 tcgccggcta ccgcaatcgg atggacacct tcttccgcag caatcccggc atgtcctcgc
     2101 gcgtcgcgca tcacatcgac tttcccgact acaccccggc gcagctccat gccatcgccg
     2161 gcctgatgct cgagcagatg caataccggc tcagcccgca ggctgacgag gcgctcaccg
     2221 aatacatcgc gcggcgcatc ctccggcctc acttcgccaa cgcacgctcg gtgcgcaacg
     2281 cgctcgaccg ggcgcggctg cgccaggcaa gccggctgtt cggccgccgg ggcggtctgc
     2341 tgtcgcgcga ggagttgctg accctcgagg cggcggacat tcgcgctagc cgcgtgtttg
     2401 cgccggagct ggatgaagag gcggcctccg gcagcgaggc cctctggtga aagctctcac
     2461 gctgacccgt ttcctgatcg aagagcagcg ccgctacccc gcagcaaccg gcggcttcac
     2521 tgcgcttgtc aacgatgtgc gcctcgcgtg caagcgcatc gcgaccgtga tcggccgcgg
     2581 cgcgctcgag gagacactcg gggcggccgg ttcgaccaac gtccagggcg aggagcagat
     2641 gaagctggac gtgctggcca acgacgtctt cgtgcgcacc aacgagtggg gcgggcagct
     2701 cgcgggcatg ctctccgagg agatgacgga tccttatctt atcccggaag agtacccgcg
     2761 cggccgttac ctgctgctgt tcgacccact ggatggctcc tccaacaccg atgtcaacgt
     2821 ctcggtcggc agcatcttct cggtgctgcg ctgtccggag ggcgtgagcg agccggtcga
     2881 gagtgatttt ctgcagcccg ggacgcgcca ggtctgcgcc ggatatgcga tctacgggcc
     2941 cgccacgatg ctggttctca cgacggggcg cggtgtcaac ggcttcacgc tcgaccggga
     3001 gatcggtgag ttcatgctca cccatccgga tctgcgcatc gatccggaca cctcagagtt
     3061 cgcgatcaat gcctccaact cgcgctactg ggaaccggcg atcaagcgct atgtgaagga
     3121 gtgcctggca ggcaggagcg ggccgcgggg acgcgatttc aacatgcgct ggatcgcctc
     3181 gctcgtcgcg gagacccacc ggatcctgat gcgcggcggc gtattcatgt acccccgcga
     3241 tcgcaaggac ctgacgcgcg ccgggcgtct gcggctgttg tatgaggcca atccgatcgg
     3301 cctgctgatc gagcaggctg gcggccgggc gagcacgggg cagcgaccaa ttctcgaagt
     3361 cgagccgcaa tcggcccacc agcgcatcgg cttcgtgttc ggtgcgcgtc acgaggtcga
     3421 aagaatcgag cgctatcacc gcgatcacaa cgaattcgag ttcgaggcgc cgctcttcgg
     3481 cacacgcggc ctgttcacca cccggatctg acagggcaga gatcgacatg tcggcgcgcc
     3541 atcccatcat cgcggtcaca ggctcctccg gggccggaac cacttcggtc atgcgaacct
     3601 tcgagcacat cttccgccgg gagaagatcc gcgccgggtt catcgaaggt gacagctttc
     3661 accgctacga tcgggcggag atgaagcagc gcctgcaaag cgcgctggac cgcggggatc
     3721 accgcttcag ccatttcggt ccggacgcca acctgttcgc cgagctcgaa gcgctgtttc
     3781 gggactacgg gcatcgcggg cggggacgct tccgccgcta tctgcacgat gaggccgaag
     3841 cttcccgtca cgcgcagccg cccgggacgt tcaccgagtg ggaggagctg accagcgata
     3901 cggacgtgct cttttatgaa ggcttgcacg gcgccatcgt cactgaggag gtgaacgtgg
     3961 cgcgtcacgt ggacctgctc atcggagtca cccccgtcat caatctcgag tggatccaga
     4021 agctgcaccg cgacaaggcg acgcgcggct attctgccga agcggtaacg gatgtcatcc
     4081 tgaggcgcat gcacgattac gtgcagtaca tctgcccgca gttctcgcgc acgcatatca
     4141 atttccagag ggtgccggtg gtggatacct ccaatccgtt catcgcccgc ttcatcccca
     4201 cgcccgatga gtcgttcctc gtgatccatt tctccaaccc gggcggcatc gactttccgt
     4261 atctcctttc gatgctgcac gactcgttca tgtcgcgcgc gaacacgatc gtctgtccgg
     4321 gaggcaagat ggatctggcc atgcagctca tcttcacgcc gatgatctgg cggctgatgg
     4381 agtgcaggcg ggaaagcctg cagcggggtg cgatggctgc atgcgggtga gaggcctgca
     4441 ggcactgata ttcgacgtgg acgggacgtt ggcggacacc gaagagctgc accgcgaagc
     4501 cttcaatact gccttccgca gcttcgactt gccgtggaac tgggacgccg ccctctacgg
     4561 caggctgctg caggtcaccg gcggaaagga gcggatcgcc cgctacatcg atgagctgcc
     4621 gatcctggaa tccgagcggg ctcgcttgaa gggccgggtc cccgatctgc atgccgccaa
     4681 gacacgtttc taccgccagc tcatcggtct gggccgggtg cgtcccaggg cgggtgtgcg
     4741 ccggctcatg ctcgaggcgc tcgaagcggg cgtacgcctc ggtattgcct ccacgacatc
     4801 gcccgagaac gtggagccgc tcatccgcgc cgcattcggt caagcgacac tcagctggtt
     4861 cgcgacgatc gcgacgggag atatcgtcca gcacaaaaag cctgctccgg atatatacaa
     4921 tgtcgcggtc gcagagctca ccgtgcccgc ctccggcgcg gtcgcagtgg aagactccgc
     4981 gatcggagtg gagtcagcca agcgtgcggg actattcgcc gtagcggttc cgtgccggtg
     5041 gacccggaca caggacttct cctcagcgga cctcgttctg gactccttgg gcgagccgga
     5101 ccacccactc gatgaagcca gtgcgcggcg gttgggcgcg aggtatctct cccttgaaca
     5161 tctggcggcg ctgcacggcg ccagcctgtc ggacaggagg cgtggggatg gccgatccac
     5221 ctgagtcttt tcgggcggca gccttcgatc tggatggaac gctcatcgat acgctgccgg
     5281 acctggctgc cgccgtgaac ctgatgctcg ggatgctcgg ggcgcgcgag ttggcgccat
     5341 cggcaatccg ggccctcgtg ggcggaggtg tcgagaagct cgtgcatcgc gccctggcgg
     5401 aaagcctggg cgagcagtcg gcgcatgcgg cgcagcgctc ggcggcagtc acattgttcg
     5461 gaaggctcta cgggcaggcg ctgttcgagc gcagccagct ctatccgggt gtcgaggccg
     5521 cgctgcgcgc gctctcggcc gccggtgtgg ggctgtgctg tgtcaccaac aaggacagcc
     5581 gctttgcgac accgctgctc gagcgggcag gcttgcgccg ctttttcgct ttcactctgt
     5641 gcgccgatac ccctgaagag cgtaagccga gtccccatct gttgctttcg gcctgttcac
     5701 ggctgggcat tgcgccggcg cagatggtgt atgtggggga ttcgcgggtg gacattgcgg
     5761 cggcgcgcgc tgcggggtgc agggtggtcg tcgtgcgcta tgggtatgac acaccggctc
     5821 ttctcgagca ggcgcaaccc gatgccatcg tggacaccct ggcggatctg ccgagcttgc
     5881 ggctctcttc tgcgccgccc gatgctacgc gcgcgccctt tgaccaaaga ggatctggcg
     5941 cgactcctcg ctagagacgg tcggctgagt gttgatctgc tgcgctttct cgtaggcgcg
     6001 gatcgtcgcg ggccgatccc ggatcgcctc gaaccagcgc ttcaggtgcg ggaaatcctc
     6061 gagcttctgg ccctgggcct catacgggac gatccacgga tatgccgcaa tgtcggcgat
     6121 ggagtattca ccggcgacga acgcacgctc ggcgagtcgc tggttgagca cgccatacag
     6181 ccggttggtc tcgttcacgt agcgctggat cgcgtaaggc agcttctcgg gcgcatagcg
     6241 cgagaagtgg tgattctggc cggccatcgg cccgaggccg gccatctgcc agaagagcca
     6301 ttgcgtcacc tcagcgcggc cgcggacatc gcgcggcagc agccgtccgc tcttctcggc
     6361 gaggtaaagc agaatcgccc ccgattcgaa cagtgaaatg ggcgctccgc cccccgcggg
     6421 ctcccgatcc acgatcgccg gaatgcggtt gttgggcgag atcgcgagga acgcaggctc
     6481 gaactgtgcc cctttcgcga tctccacggg cacgatgcgg taggggagcc cgatctcctc
     6541 gagcatcatc gtgatcttgt gcccgttggg cgtcgtccag tagtacaggt cgatcatgac
     6601 cgcctcggct gatgggagtg ctgagacact cgcacgaacg gcagcgcctg tgccagtgtc
     6661 attggcgggt gcggggcacc cgccatcgtc ggtattgacg cccgggaaaa gctgacactc
     6721 gggccgctgg aaggtcgcct gcggcgactt cccaccgcag aacttagctt ggcttcgccc
     6781 ccggcttgtt ggcacccttg gtgatggcct gcgtgctgct caccttgagc tcattggtcg
     6841 cgccgggcac ctcgtcggcg aaatacagcg ggcgcgcgcc ctcaccggta tcgggctcga
     6901 ccgcaaagag cagatcggcc atgtcatcgg cgtgctcttc ctccttggcg aggatttgct
     6961 cgatgaggcg gcgcgaggtc gagtccttct cgccgaagaa cttcgagatt tcccggtaag
     7021 tctcgatggc gatgcgctcg gcaatcagat cctcgcggat catgtccgcc agcgaggtcc
     7081 cttccttgta ttcgctgtgg ctgcgctcgg tgaggatcgc gggattcata tccggcttgc
     7141 cgccgagctg cttgatgcgc tcggcgatcc agtcggcatg ctcctgctcc tcctcggcat
     7201 gctccttgaa ctccgccttg acggcctggg aatgaatccc cgaagccatg aagaagtgga
     7261 accggtaccg cagcacgcac accagctccg tcgcgagcgc ctcattcagt atgccaatcg
     7321 ccttctggcg atcgagctgg tagtcctccg tcacggcacc ttccgtgatc tgcttgcggg
     7381 cgcggtcgcg aatctgctgg atctgcgtga tatggctcat gggttctcct tcggtttcgc
     7441 gtaggcttag caagaaacgc acccacgttc aaggtcggcc gcagcagcag ggggcgtgcg
     7501 ttctagcccg ctatggggat gccgtctatc caggtgtcga gcacctggag ctgctcgtcc
     7561 accagcacca gattggcgcg atagcccggc gcgatgcgcc ccacctgatc cgatagcccg
     7621 aggaactgcg ccggatacag actggccatc cgcacggcct cgctcaatgg caatcccaac
     7681 atcgagacgg cattgcgcac acagctcgcc atatccacgt ttgagcccgc gagccggccg
     7741 tcctcatcta cacagaggct gccggagacg gtgatgtgcc ggccctgaag attgaatgac
     7801 ttcgtgtcgg tcccgacact cggcatagcg tcggtcacca gcatgaagcg gtcatgacgc
     7861 ttgcagcgca gcgcgatgcg cagcacgacg gggtcagtgt gcgctccatc cacgatgatg
     7921 ccgcaccagc tctgctgatc gtccagggca gcccccacca cgccaggctc gcggcctgtg
     7981 agctgggaca tcgcgttgaa caaatgcgtg aagccactca gtccatggcg cagagcgctg
     8041 gcgatttccg catacgtcgc attcgtgtga ccggcggaga ccaccacacc tgcgctcacc
     8101 agcgtgcgga tgagctccgg cgtggtcatc tccggcgcca gcgtcaccag tgtccggccg
     8161 ccgcgcaatg aagtgagcag cccgatgcca cttgcgtcga gctcgcgcag cttcgcctga
     8221 tcgtgtacgc ccttgcgggc cacattgaga aacggtcctt cgatatggat gccgagaaca
     8281 ccgggcacgc cggccttgat tgcggcctgc accgcgccga tggcccgggc gaccacctcg
     8341 agatctgcac tgatcagcgt cgggagaaag cccgtcgtcc caaactttcg gtgcgcgatg
     8401 ccaatgctgc ggatgacctc gaggctcgga gagtcgttga acagcacgcc cccgccacca
     8461 ttcacctgac agtcgatgaa acccggcagc agcagtgcgc cgccgaggtc gcgccgctcc
     8521 aattgggcgc agcgcggatc cgactccggc acgagccctg tgatcaggcc cccctcgagc
     8581 atgacggcgt ggttctcgat cagaccggca tcacccatga cctgcccgtt gatgagcgcc
     8641 gtggccatca gacggtctcg gtaatcttgc gcaagtgagg cggccgatcc ggatcgtagc
     8701 cccgcgcgag tgccagcgag ttgacgaggc ggtagaagct ctgaatgagc agcatcggct
     8761 gaatcgctgc gggtgcctcc tcgctcggta aaacgagcgc ctgcggggcc cttgcgcctg
     8821 cgatcaccac ctcggcaccc cgcgaggcga tctccgctgc gagcgactcg acgctcgcat
     8881 gcgtctcatc gctctgcgca aagatcagca ccggaaagcc gggcttgacg agtgcgatgg
     8941 gtccatgacg cagctccgcc gcgctcagcg cttcagcatg aatcccgcac gtctctttca
     9001 acttcaacgc ggcctcttgc gcgatgccca gacccagccc gcgaccgacg acaaacagat
     9061 gatccgcccc ctgaaggcga gacagcgcgc tgctccaatc gcgctcccag gctcgttcga
     9121 gcgattcagc ggcgcgccga agctgctcga gcaagcgccc atcctgcgcc caggcggcca
     9181 cgagatgaat gacggcggca atcgaggcga tgtacgactt ggttgccgcg acgctgcgct
     9241 cttcgcctgc ccgcagcggg atagcgatgt cagccgcgcg tgccagcggt gaggattcgg
     9301 cattcaccag cgcgacgatc agcgaacctg cagcccgggc gctgtccacg gcggcgagca
     9361 gatcgggact ggcccccgat tgggagatgg cgagcagcag catgtcacgc aactgaggct
     9421 gcgcttcgta caacgagctc accgacgggg cagccgatgc agtcacgagg ccgagacgtg
     9481 tctcgatgag gtatttcgcg aaagtggccg catggtccga gctgccgcgt gcgcacgtga
     9541 ccacagcctt gggcccaagc ccacgcagcc gagcacccaa ctcacccatc aacgactcat
     9601 tggcggcaaa ctgctgccgg acaatcgcag ccgcctgcgc ggcctcgcgg aacaggctgg
     9661 tggactcggg cctcgaggtc gtgtttgggc tgatcacgaa gagatgctga gctcagccac
     9721 gaagtcgtag gtatcaccgc gatagtagga ctgcgtgaac tctgcggctc ggccgtcctg
     9781 gaggaagccg agccgctcga ccagcagacc ggcgtcgcgc tccttggcct ggagcaattc
     9841 cgcctgatcg cccgtcagca gcaccgcccg aagcctctgc agtgcacgca ccggtcgatt
     9901 gcccgcacgc tcgagagcct catacaggga ggattcgacc gcctcgagcg agggcagcga
     9961 ggaggcgagc acggttgcat attcgagcga catcggcgag tcgtttgcga aacgtatgcg
    10021 atgaaaacga aacaccgggg tgccggggct cgagcgcagt gtcagtgcct cttcaggcga
    10081 caccgtaccg gcggagcgtt gcagccagac gctgcgtggc gtgcgaccgc gtgcgcgcat
    10141 gtcctcggag aacgaggtca gtttcgagaa gttcttctct acccgcgcgg tgacaaaggt
    10201 tcccgaccct tgccggcgga cgagcagccc ttcgctgacg agtccgtcga ttgccttgcg
    10261 cactgtgatg cgtgagacgg agaactccgt cgccagatcg cgttcggcgg gaagcgcatc
    10321 gtccgggccc agcacgcggc gctcgatagc ttggcgcagc gcacgctgaa gctgctggta
    10381 gagagggagg ctgctcgatt catccagtgc cccgacggtc gagagcagat tcaagcggtg
    10441 tctcctgaag atccgatcac gtcatactac caatatcaga ccactttaaa cctgaagaag
    10501 cttattggct agcacaaccg attgttttgg caagtcatag gctcgtatgg ccagattggt
    10561 attcatggag gatagcattc ccgccagaat ggtactatat tggaacctaa gtggtccctt
    10621 gcgcggttcg gctgatatga cgaggccgtg acggcgccgg atcacgggga gcaacgcggc
    10681 agcgcggttg taacgaataa cagtggggtg atcattttat gagaatcgca aggtctacct
    10741 tggtcggaac ggccgtggcc atggctttgt ttggtcgtaa tggcgcagtc cgggctgatg
    10801 cccccgatac cacctccagc cagaacgggc cgctcgagga agtggtcgtc catggctacc
    10861 gcatggcgct gcgcgagtcg atggagacca agcgcgcggc gaccggtgtc gaagaggtg
//
LOCUS       DASRHN010000137         6201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1200186, whole genome shotgun sequence.
ACCESSION   DASRHN010000137 DASRHN010000000
VERSION     DASRHN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200186"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VFB37_05245"
     CDS             complement(<1..1473)
                     /locus_tag="VFB37_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21891.1"
                     /translation="MLDDEIDSTPSLRRRRQWVGVRLVLAVAAGVAVFGVFLLVAYEE
                     AIARIPEHRAALERLVRAQTGLDVRFNELGVRWGWYGPEAVFRRVELDEPGTSRVLLR
                     APELVVGFDAWRTLRSGHPEAGRIELVAADIDFAGLEPGVTPHTAPKPSLHPGFGNAH
                     PATDTRFDRMAILQRWRGVRIDLDGGTLRLPDPGGSATPFTLQIRRASLRRSDEEWSA
                     YGLVFLPDRIGRMARVVMRVTGDLNQPGTLSGSVRLEAHRLLFSGCRDLLAPMTGLAA
                     YLPRAGSGDLTTDVDFAGGSVIKATGNLRAAGLVFAGLQSKASRRQEAHEEASGDVDE
                     LTLERLRGDFRLARSASEWRLRVDSLEAGRSRRQASLRLDAARSGEWIRGTLEGAALK
                     PIVAVARWMRLPMDLAGIRLNGLVREVGFDWSELRPEGDKLQLSARMQDITLMPRSGD
                     FSLSGFDLQLQGSESLQTAELRSRSAHLELAQSRQYPLSDI"
     gene            complement(1518..2984)
                     /gene="rng"
                     /locus_tag="VFB37_05250"
     CDS             complement(1518..2984)
                     /gene="rng"
                     /locus_tag="VFB37_05250"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HZO21892.1"
                     /translation="MSVEILVNVAPRETRAAIVETGVLQEIYVERTSKRGLVSNLYKG
                     RVSRVLPGMQAAFVDIGLDRTAFLHVADIADGDPEDTVILPTVGDIRRLVSPGDDILV
                     QVIKDPLGTKGARLTTFIALPSRYLVYLPRGEGIGVSSRIDDEAERQRLREAVSGSIA
                     GELSGGYILRTAAQGVPVESLREDMAYLGKLWEHVRTRALQTQAGSIVHEDLRLALRV
                     LRDELSRGVSRVLVDSPREHAHMVAFAAAFMPDAATRIELHAGPRPIFDLHGIEDEIA
                     RALERKVTLKSGGHLVIDQTEAMTTIDVNTGAYVGHRNLEETIFRTNLEAAVAIARQL
                     RLRNLGGIIIIDFIDMRDESHRKQVLAALERALAGDRAQTHIVSLSPLGLVEMTRKRT
                     RESLEHLLCQPCPACEARGFVKSAETVCNEIFREIVRQARQFASRELLILAHQDVLER
                     LLEDESATLAELETQVSRPIRMQVEALYGVDQYDVVPI"
     gene            complement(2981..3580)
                     /locus_tag="VFB37_05255"
     CDS             complement(2981..3580)
                     /locus_tag="VFB37_05255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZO21893.1"
                     /translation="MDSSAPVLCLASLSPRRRELLAQIGVNCSVVGADIDESVLAEEG
                     PRDYVIRLAQQKALAVRRGGETLPVLAADTTVLLEGRIYGKPKDQQDSLAMLRQLSGR
                     THEVLTAVALADSQGVVARLSQSTVVFRKLTLTECAAYWDSGEPRDKAGSYAIQGLGA
                     VFVESLSGSYSGVMGLPLFETAELLRASGIAYWSGRVPP"
     gene            complement(3589..4059)
                     /gene="rlmH"
                     /locus_tag="VFB37_05260"
     CDS             complement(3589..4059)
                     /gene="rlmH"
                     /locus_tag="VFB37_05260"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZO21894.1"
                     /translation="MKLRILAVGSRMPAWVRQAFEEYVARLGPAAKLTLTEIAPGLRT
                     AGASAHKAIATEARRLLAALREGEFVIALDERGTQMSTLELARWLQARRRDGADLAFL
                     IGGPDGHAPEVLARSQFTWSLSLLTLPHALVRVVLAEQLYRAHAVLGNHPYHRQ"
     gene            complement(4133..4555)
                     /gene="rsfS"
                     /locus_tag="VFB37_05265"
     CDS             complement(4133..4555)
                     /gene="rsfS"
                     /locus_tag="VFB37_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZO21895.1"
                     /translation="MISRTRPRRSGEPERVLLQEVITTALDDMKAVNVKVLDVRGLTD
                     IADTMVIASGNSDRHVRSIAERVLEKAKAAGFRALGTEGERDGEWVLVDLQDIVLHVM
                     LPRVREFYGLERLWDHAEAPVLAAAQVPPTTARRGRRR"
     gene            complement(4552..5196)
                     /gene="nadD"
                     /locus_tag="VFB37_05270"
     CDS             complement(4552..5196)
                     /gene="nadD"
                     /locus_tag="VFB37_05270"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZO21896.1"
                     /translation="MMQPIGLFGGTFDPIHYGHLRTAFELWQELRLTEVRFLPTGSPP
                     HREQTHATAELRLAMVRAAVAGQPGFVVDDREVRRTGVSYSIDTLTELRAELPDRSLC
                     LLLGMDAFLGLPNWHRWRELLNLAHIVVAHRPGWKAPTKGPLGEVMVDHGTGTVRDLH
                     DKPGGRVYVHAVTQLEISSTELRQLIIAGHDPRYLVPEAVRQIIRETGCYARHT"
     gene            complement(5205..>6201)
                     /gene="holA"
                     /locus_tag="VFB37_05275"
     CDS             complement(5205..>6201)
                     /gene="holA"
                     /locus_tag="VFB37_05275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZO21897.1"
                     /translation="ALSAHLAQHLSPLYLISGDEPLLAAEAADAVRERARSTGFSERK
                     VHFPERAADWEEVRGSLGNLSLFGSRQLVEIRLASARVGTAGASALLSIFEMEDPDTV
                     LLILAPRLDREVQSASWVKHVQMKGAWVQVWPVAADKLVSWLRARSRRLGLELAPEAL
                     ELLAERTEGNLLAAHQELEKLALIAPGQPITADAVLKSVADSARFDVFQLGESVLAGD
                     AERALRMLAGLRAEGTEPTLVLWTLTKAMRDLWSTLSGGADAGSRAWSRQLAALQKGA
                     RRALRLRFEELATRAGRADRMVKGRLAGNAWDEIALLALEICARPAVRPAETVLK"
BASE COUNT         1078 a         2124 c         2011 g          988 t
ORIGIN      
        1 aatgtccgag agcgggtact ggcgcgattg cgccagctcc agatgagcgg agcgcgagcg
       61 caactccgct gtctgcagag actcgctccc ctgcagctgc aggtcgaatc cgctcagaga
      121 gaaatcgccc gagcgcggca tcagcgtgat gtcctgcatc cgggcggaca gttgcagctt
      181 gtcgccctcc ggacgcaact cgctccagtc gaatccgacc tcccgcacca ggccgttgag
      241 ccggatgccg gccagatcca tcggtaggcg catccagcgg gcgacagcga cgatgggttt
      301 cagtgccgcc ccctccaagg tgccgcggat ccattcgccg gagcgagcgg catcgaggcg
      361 cagcgaagcc tgtcggcggc tgcgcccggc ctcgagcgaa tcaacgcgca gccgccactc
      421 ggatgccgag cgcgccaggc ggaagtcgcc ccgcaaacgc tcgagggtca gctcatcgac
      481 atcgcccgag gcctcctcgt gagcctcctg gcggcgtgag gccttgctct gcaggcctgc
      541 aaacaccagc cctgctgcgc gcaggttgcc ggtggccttg atgacggagc caccggcgaa
      601 gtcaacatcc gtggtgaggt cgccgctccc ggcgcggggc agataagctg ccagcccggt
      661 catcggggcg agcaggtcgc gacagccgga gaaaagcagg cgatgcgcct cgagccgtac
      721 cgatccgctt agcgtaccgg gctggttcag atcgccggtc acgcgcatca ccactctcgc
      781 catgcggccg atgcggtcgg gcaggaacac cagcccgtag gcgctccact cctcgtccga
      841 gcggcgcagc gaggcgcggc ggatttgcag agtgaagggc gtcgccgagc cgcccggatc
      901 ggggagccgc agcgttccgc cgtccagatc gatgcgaact ccgcgccagc gttgcaggat
      961 ggccatgcgg tcgaagcgcg tatcggttgc gggatgcgcg ttcccgaacc ccggatggag
     1021 gctcggtttc ggcgcggtgt ggggagtcac tcccggctcg agcccggcaa agtcgatatc
     1081 ggcggcaacc agctcgatgc gtcccgcctc ggggtgtccg ctgcgcagag ttcgccaggc
     1141 atcgaaaccc accacgagct ccggggcgcg cagcagcact ctggaggtgc ctggctcatc
     1201 cagctcgaca cgtcgaaaga cagcttcggg gccgtaccaa ccccagcgca ctcccaactc
     1261 gttgaaacgc acgtcgagtc ccgtttgcgc acgcaccagc cgctcgagcg cagcccgatg
     1321 ctcggggatg cgcgcgatgg cctcctcgta cgcgacgaga aggaaaaccc cgaagacggc
     1381 gacaccggcg gccaccgcca gaaccaaccg cacgccgacc cactgccgcc tccggcgcaa
     1441 gctcggcgtg gaatcgattt cgtcatccag cattgcagat gccacggcgc gttgcggcgc
     1501 cccgtttttc ccctgatcta gatcggaacg acgtcgtact gatcgacccc gtagagcgcc
     1561 tcgacctgca tccggatcgg ccgcgatacc tgggtctcga gctcggccag cgtcgccgac
     1621 tcatcttcga gcagccgctc gagcacatcc tggtgagcca gtatcagcag ctcgcgcgag
     1681 gcaaactgcc gggcttgtcg cactatttcg cgaaagatct cattacacac cgtttcggcc
     1741 gatttcacga acccgcgagc ttcgcaggcc gggcacggct ggcacagcag atgctcgaga
     1801 ctctcgcgcg tgcgcttgcg cgtcatctcc accagaccca gcggcgaaag gctcacgatg
     1861 tgggtctggg cgcggtcgcc tgcgagtgcg cgctcgagcg cggccagcac ctgcttgcgg
     1921 tgcgattcgt cccgcatgtc gatgaagtcg atgatgatga tgccgccgag attgcgcagc
     1981 cgcagctgcc gggcgatcgc caccgccgcc tcgaggttgg tccggaaaat ggtctcctcg
     2041 agattgcgat gccccacata agcgccggtg ttgacatcga tggtggtcat cgcctcggtc
     2101 tggtcgatta ccaggtgtcc accggacttc agcgtcacct tgcgctcgag ggctctggca
     2161 atctcatcct cgatgccatg cagatcgaag atgggccggg gtcctgcatg caactcgatg
     2221 cgcgtggcgg catcgggcat gaacgcagcg gcgaaggcca ccatgtgggc gtgctcacga
     2281 ggcgaatcca ccagaacccg gctgacaccg cgagagagct cgtcacgcag cacgcgcagc
     2341 gccaggcgca gatcctcgtg aacgatcgag cctgcctggg tctgcagcgc acgcgtgcgc
     2401 acgtgctccc agagtttgcc cagataagcc atgtcctcgc gcaggctctc gaccgggacg
     2461 ccctgagcgg cagtgcgcag gatgtatccg ccggagagct ccccggcgat ggagcccgac
     2521 actgcctccc tgagccgctg acgctcggct tcgtcatcga tgcgggacga gaccccgatc
     2581 ccctcgccgc gcggcagata cacgagatat cgcgatggca gcgcgatgaa tgtcgtcagt
     2641 cgcgcgccct tggtcccgag cggatccttg atgacctgca ccaggatgtc atctccggga
     2701 ctgacgagcc ggcggatgtc acccaccgtg ggcaggataa cggtgtcctc cggatcgccg
     2761 tcggcgatgt ccgccacgtg aaggaaggcg gtgcgatcga gtccgatgtc cacgaatgcg
     2821 gcctgcatgc ccggcagtac gcgcgacacc cttcccttgt agagattgct cacgagcccg
     2881 cgcttgctcg tgcgctccac atagatctcc tgcaatacac cggtctccac gatcgccgcc
     2941 cgcgtctcgc gtggtgcgac gttgaccagt atctcgacgc tcacggcgga acccgtccgg
     3001 accagtaggc aatgcccgat gcgcgcagaa gctcggcggt ctcgaacagc ggcaatccca
     3061 tcacccccga atagctcccg ctcagggact cgacgaacac cgcgcccagt ccctgaatgg
     3121 catagctgcc cgctttgtcg cgcggctcgc cgctgtccca gtaggcggca cactctgtca
     3181 gtgtcagttt gcggaacacg accgtgctct ggctcaagcg cgcgaccacg ccctgcgaat
     3241 cagccagggc tacggcagtc agtacttcgt gggtgcgacc ggagagctgg cggagcattg
     3301 cgagtgagtc ctgctggtct ttgggtttgc cgtagatgcg gccttccagc agcacggtgg
     3361 tatcggcagc gagaaccgga agagtctcac cgccacgtcg aactgcaagc gccttctgtt
     3421 gggccagccg gatcacataa tcacgggggc cttcctctgc cagcacgctc tcatcgatgt
     3481 cggctcctac cacgctgcag ttcactccga tctgcgccag cagctcgcgg cgcctgggcg
     3541 agagcgaggc gaggcagagc accggagcgg aggagtccat gcggcccatc actggcgatg
     3601 atacggatga ttcccgagca cggcatgagc tcgatagagc tgctcagcca acaccacacg
     3661 caccagagcg tgaggcaagg tcagcagcga cagggaccac gtgaactgac tgcgggcgag
     3721 aacctcgggc gcatgaccgt cgggtccgcc aatgaggaag gccaggtccg ccccgtctcg
     3781 ccggcgagcc tggagccagc gcgccagctc gagggtgctc atctgggtgc cgcgctcgtc
     3841 cagcgcgatg acgaattcgc cctcgcgcag ggccgcgagg aggcggcgcg cctcggttgc
     3901 gatggccttg tgcgccgatg cgcctgcagt acgcaggccc ggcgcgattt ccgtcagcgt
     3961 gagtttggcc gctggtccca gccttgcgac atattcctcg aatgcctgtc gcacccaggc
     4021 gggcatccgt gagccgacgg caaggatgcg cagcttcatg ggcggaccga gccaaggagg
     4081 cgggcaagcg gcccgtccac gagccccttg gcgagcagcg gcagaaggcc tgctatcgcc
     4141 gccgtcccct gcgtgcggtg gtgggaggaa cctgcgcagc cgcgaggacc ggtgcctcgg
     4201 cgtgatccca gagccgctcg agaccgtaga actcccgcac tcgcggcaac atgacgtgca
     4261 ggacgatgtc ctgcagatca accagaaccc actcgccgtc gcgctcgcct tcggtgccca
     4321 aagcgcgaaa gccggcggcc ttggccttct cgaggacgcg ctccgctatg gagcgcacat
     4381 ggcgatcgga gttgccggag gcgatgacca tcgtatcggc aatgtcggtg aggccacgaa
     4441 cgtccagcac cttcacgttg accgctttca tgtcatcgag cgcggttgtt atgacctcct
     4501 gcaacaggac gcgctcgggc tcaccggagc ggcgcgggcg agtacggcta atcatgtgtg
     4561 gcgagcatag catcctgttt cgcggatgat ctgacggact gcctcgggca cgagatagcg
     4621 tggatcgtgg cccgcaatga tcagttggcg cagctcggtc gaggaaatct cgagctgtgt
     4681 cacggcgtga acgtatacgc ggccgcccgg cttgtcgtgc aggtcccgca cggttccggt
     4741 gccgtggtcc accatgacct cccccagcgg ccccttggtg ggggccttcc agcccggtcg
     4801 gtgcgccacc acgatgtgtg ccaggttgag cagctcgcgc cagcggtgcc agttgggcaa
     4861 acccaggaac gcgtccatcc cgagcagaag acaaagcgag cggtccggca gctccgcgcg
     4921 cagctcggtg agggtgtcga tggaatacga aactcccgtg cggcggacct ctcgatcgtc
     4981 caccacgaat cccggctggc cggcgactgc ggcgcgcacc atcgccagtc gcagctccgc
     5041 ggtggcgtga gtctgctcgc ggtggggcgg actgcccgtt ggcaggaatc tcacttcggt
     5101 gagccgcaac tcctgccaca gctcgaacgc cgtacgcagg tgcccgtaat gaatcgggtc
     5161 gaacgtcccg ccgaacagcc ctatgggttg catcatggcg ggggctattt gagcacggtt
     5221 tcggcgggcc gcaccgccgg gcgagcgcag atctccaggg ccaggagcgc gatctcgtcc
     5281 caggcatttc cggcaagccg tcccttgacc atgcgatcgg cccgcccggc gcgggtcgcg
     5341 agctcttcaa aacgcaggcg caaggcgcgc cgcgcaccct tctgcaacgc ggcgagctgc
     5401 cgcgaccagg ctctcgaacc tgcatcggct ccgccgctca aggtgctcca cagatcgcgc
     5461 atcgccttcg tgagcgtcca cagaaccagc gtcggctcgg taccctccgc ccgcaatccg
     5521 gccagcatgc gcagcgcccg ctcggcatcg cctgcgagca ccgattcgcc gagctgaaag
     5581 acatcaaagc gagcggagtc ggccacgctc ttgaggaccg catcggccgt gatcggctgc
     5641 cccggggcga tcagcgcgag tttctccagc tcctgatgag cggcgagcag gttgccttcg
     5701 gtgcgctcgg ccagcagctc gagcgcctcc ggggcgagct cgagtcccag gcgccgcgag
     5761 cgtgctcgca gccagctcac cagcttatcg gccgcaaccg gccagacctg cacccaggcg
     5821 cccttcatct gcacgtgctt cacccagctc gcgctctgca cctcgcgatc gagtctcggg
     5881 gcgagaatca ggagcacggt gtcaggatcc tccatctcga aaatggagag cagcgcgctg
     5941 gctcctgccg taccgaccct ggcgctcgcc agccggatct ccacgagctg gcgcgagccg
     6001 aacagggaga gattgccgag cgacccgcgc acctcctccc aatctgcggc gcgctcgggg
     6061 aagtgcactt tgcgttcgct gaaaccggtg ctgcgcgcac gctcgcgcac cgcatcggcc
     6121 gcctctgctg cgagcagtgg ctcatccccc gagatcaggt acaggggtga cagatgctgc
     6181 gcgagatgag cggaaagcgc g
//
LOCUS       DASRHN010000138         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_536902, whole genome shotgun sequence.
ACCESSION   DASRHN010000138 DASRHN010000000
VERSION     DASRHN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536902"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFB37_05280"
     CDS             <1..395
                     /locus_tag="VFB37_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO21898.1"
                     /translation="PRELVARVRAVLRRMSGEGHPANGAMQWGPLRLDLRARRADVND
                     QDLELTAAELRILELLVRADTKTVTRDELMTQALGRRLLPTDRSLDTHVSNLRRKLAK
                     VTARVSVQSVRGTGYALALSARTDDSEP"
     gene            403..1845
                     /locus_tag="VFB37_05285"
     CDS             403..1845
                     /locus_tag="VFB37_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO21899.1"
                     /translation="MHSLYWRIFLAFWLALVLILLGTVTLVVNNELQRRFSQPWVQRA
                     ELYAQATQAFQSGGGPALRAWLVGLRPEIFALTYITDQNTGKEMLGRPIPDYLRDPPE
                     RIAAARQNALLHPTIRSVGGPLVLVGPGGQAFHVLVGPLRSGPHLFGELEMPGVSTAT
                     LAIALVVSAAVCFFLARYLVGPVDQLRRATRQIASGDLSVRVSPKLKGRRDELGLLAS
                     DLDMMSERVRNLLEVKQQLLRDVSHELRSPLARLQLALSLARRQDNTEVERHLARIAC
                     EGDRLEQLIARTLRLASLERPMQGFEHLPIDLAELIANIVADVAIEAEAHGCSVALES
                     ENDLGVRGDTELLRSALENVIRNAVRYGPPSSEVRITARRAETRIEVIVRDNGPGVPE
                     KDLRLIFEPFYRVDAARNRAVGGDGLGLAIAARAISIHGGTIEARNLSAGGLAVHVSL
                     PADESEGVTRRESRPELQSAAVPAANNVIA"
     gene            complement(2226..2894)
                     /locus_tag="VFB37_05290"
     CDS             complement(2226..2894)
                     /locus_tag="VFB37_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21900.1"
                     /translation="MLSVRGTRPVPRWVVILSVLVLLIGILLAIAPVRHALLRGAGHA
                     LVKNDRIEPADVIVIAADARAVGALEAADLVREGISRRIALFFYEPDRADRELARRGA
                     PTVDLNTLTLEQLHAMQVHAAIEVIPGSIAGTTDEGEILGRWCRERSIRTLLFVSTTD
                     HSRRARRVLNRALADYGTRVIVHASRYSSFDPDSWWQSRDGRRTELTESEKLLLDLLR
                     HPFS"
     gene            complement(2969..3790)
                     /locus_tag="VFB37_05295"
     CDS             complement(2969..3790)
                     /locus_tag="VFB37_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZO21901.1"
                     /translation="MHLQTTVAGAAYAPAGRLDDAKQGARDLVHLVDDFDQKQFLAVA
                     AAFGQRRYGFVVTPNVDHLIRLHEDPSFRALYRSAEYVLMDSRFFARVLRLLKGIRLP
                     VCTGSDLSEGLLAQVAAKSDRIVMVGGSAEQAQRLAALYGLTNLRHHSPPMGFIEDPL
                     AVEECLQFIESESPFRFCFLALGSPQQEMLAQLLLARGAARGLALCVGASLNFITGVE
                     KRAPLWMQRASLEWLHRLWLDPRRLAGRYLVRGPRIFSHLRRARFVLRNPSANSG"
     gene            complement(4061..>4399)
                     /locus_tag="VFB37_05300"
     CDS             complement(4061..>4399)
                     /locus_tag="VFB37_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZO21902.1"
                     /translation="GRLLRATSLDELPQLLNVLEGKMSVVGPRPHAVAHNELYRKLIS
                     GYMIRHKVRPGITGLAQVNGLRGETETLDKMRERVRYDLEYLSHWSPWLDLKIILKTI
                     WVIARDKNAY"
BASE COUNT          803 a         1401 c         1421 g          774 t
ORIGIN      
        1 gtccgcgcga gctggtggcc cgtgtccggg cggtgctgcg tcgcatgtcc ggcgaagggc
       61 atcccgcgaa tggcgccatg caatgggggc cgctgcggct cgatctgcgg gcccggcgcg
      121 ccgatgtgaa cgatcaggac ctcgagttga cagccgcgga gctgcgcatt ctcgagctgc
      181 tcgtgcgagc cgataccaag acggtgacgc gcgatgagct catgacccag gcgctcggac
      241 ggcggttgct gcccaccgac cgcagcctcg acacgcatgt gagcaacctg cgcagaaagc
      301 tggcgaaggt caccgcccgc gtcagtgtcc agagcgtgcg gggcaccggt tatgctctcg
      361 cgctgtcggc tcggacggac gacagtgaac cctgacctca gaatgcattc gctctattgg
      421 cggatatttc tcgccttctg gctcgcgctg gtcctgattc tgctgggaac cgtcaccctc
      481 gtcgtcaaca acgagctgca gcgccgattc tcgcaaccct gggtgcaacg ggcagagctg
      541 tatgcccagg ccacgcaggc gttccagtcg ggcggtgggc cggccttgcg cgcctggctc
      601 gtggggctgc gtccagagat ctttgcgctc acctacatca ccgatcaaaa caccggcaag
      661 gagatgctcg gacggccgat cccggactat ctgcgcgacc cgccggagcg catcgccgcc
      721 gcccggcaga acgcactgct gcatccgacg atccgctcgg tcggcggtcc gctggtgctg
      781 gtgggccccg gcggccaggc ctttcacgtg ctcgtggggc cgttgcgatc cgggccgcac
      841 ctgttcggtg agctggaaat gcccggcgtc tcgacggcga ccctggcgat tgcgctcgta
      901 gtgagcgccg cggtctgctt ctttctcgcc cgctatctgg tcgggccggt ggatcagctc
      961 aggcgcgcca cgcggcaaat cgcttcgggc gatctgagcg tccgggtgtc gcccaagctc
     1021 aagggtcgcc gcgacgagct ggggttgctc gcctcggatc tcgacatgat gtccgagcgc
     1081 gtccgcaatc tcctcgaggt gaagcagcag ctgctgcgcg atgtgtcgca tgagttgcgg
     1141 tcacctttgg cgcggctgca gcttgcgttg tcacttgccc gtcggcagga caatacggag
     1201 gtcgaacgcc acctcgcccg catcgcctgc gaaggcgatc gtctcgagca actcatcgcc
     1261 cgcaccctgc gcctcgcgag cctcgagcgt ccgatgcagg ggttcgagca tctccctatc
     1321 gacttagcgg agctgatcgc caacatcgtg gccgatgtcg ccatcgaggc cgaggcgcac
     1381 gggtgcagcg ttgcgctgga gtcggagaac gacctcggcg tgcggggcga caccgagctt
     1441 ctgcgcagcg cgctggagaa cgtgatccgc aatgcggtcc gctacgggcc gcccagctcc
     1501 gaggtgcgga tcaccgcacg gcgcgcagaa acgcgcatcg aggtcatcgt gcgggacaac
     1561 ggaccgggcg tgccggagaa agatttgcgg ctcattttcg agccatttta ccgcgtcgat
     1621 gcggctcgca accgcgcggt gggcggcgat ggtctcggac ttgccatcgc ggcacgggcc
     1681 atctcgattc atggcggcac catcgaggcg cgcaacctga gcgccggcgg gctggccgtc
     1741 catgttagtt tgccggcaga cgagagcgag ggcgtgacgc gaagggaatc ccggcctgag
     1801 ttgcagagtg cggcagtccc cgcggccaac aacgttatcg cgtaggctgg ctttcaggct
     1861 gtcgcctggc agctcagaag gatttgagcc caagaaaaag ccgcccttcc gggcggcttc
     1921 caacggcgct caatgacgag aactggcagc aacgcctgag cctcagggag ggtgaaaaac
     1981 tcgggcgcga ataccacacc gggacaaaaa atcgacactc aatcagtcag gtcttgcgaa
     2041 cctcacgcga ttagacgcct cgcggggcaa aaagttgacc cgattcaacc ttcttatgaa
     2101 ttattgttct ctttaacatt cctgcggtca acgcggcgca cgcgctgaaa gctgcgggaa
     2161 aggtgcagcg cagcgaacca acatcaaggt gaagcgtggc gttgagagaa gcggcccgcg
     2221 ccgcctcagg aaaagggatg tcgcaggaga tcgagcagca gcttttccga ttcggtcaac
     2281 tcggtgcgcc gtccatcgcg ggactgccac cacgaatccg gatcaaagct cgagtagcgt
     2341 gaggcgtgca cgatcacacg agttccatag tccgcgagag cgcgattcaa cacgcgccgc
     2401 gcccgccgcg agtgatcggt ggtgctgacg aacagaagcg tccggatcga gcgctcgcga
     2461 caccaacgcc ccagtatctc cccttcgtca gtggtgccgg caatgctccc gggaatcacc
     2521 tcgatcgcgg cgtgcacctg cattgcgtgc agctgctcca gagtgagcgt attcaaatcg
     2581 accgtcggtg cgccgcgacg cgcgagctcg cgatcggcgc gatcgggttc atagaagaac
     2641 agtgcgatgc gcctcgagat gccttcgcgt acgagatccg cagcctcaag cgcgcctacg
     2701 gcgcgcgcat cggccgcgat gacgatgaca tcggcaggtt caatgcggtc gttcttcacc
     2761 agcgcgtgac ctgcgccgcg caggagggca tggcggacgg gagcgatcgc caggaggatg
     2821 ccgatcagga gcaccaggac tgagaggatg acgacccagc gcgggacggg ccgggtaccg
     2881 cggacggaca gcatcgtgct tctattatgg cgcgagagcg ggaaacgggc ggcgatgccc
     2941 cagaggccaa cgccgctcgc gcaaaggctc agccggagtt agcgctcggg ttgcgcagca
     3001 cgaatcgagc gcggcggagg tggctgaaga ttctcggtcc gcgtaccagg tagcgtcccg
     3061 cgagccgtcg aggatcgagc cacagccggt gcagccattc cagtgaagct cgctgcatcc
     3121 aaagcggcgc ccgcttctcc acgcctgtga tgaaattcag tgaggcgccc acgcacaatg
     3181 caagcccccg ggccgcaccg cgcgccagca gcagctgggc cagcatctcc tgctgcggcg
     3241 agcccagcgc caggaagcag aagcggaaag gactttcgct ctcgatgaac tgcaggcatt
     3301 cctcaacggc cagcggatcc tcgatgaatc ccatcggcgg gctgtgatgg cgcaggttcg
     3361 tcaggccata gagggcggcg agcctttggg cctgctcggc gctgcctccg accatcacga
     3421 tgcgatcgga tttggcggcg acctgcgcga gcaggccctc gctcagatcg gaaccggtgc
     3481 ataccggcag ccggatgccc ttcagcaggc gcagcacgcg cgcgaagaag cggctgtcca
     3541 tcagcacgta ctcggcgctt cgatagagcg ctcggaagga cggatcctca tgcaggcgga
     3601 tcaggtgatc gacgttcggc gtcaccacga aaccgtaacg ccgctgcccg aacgccgcgg
     3661 cgacggccag aaactgcttc tgatcgaaat cgtcgacgag gtgtacgagg tcgcgtgcac
     3721 cttgctttgc atcatcaagt cgccctgcgg gcgcgtacgc ggctccagcg acagtcgttt
     3781 gcaggtgcat tccattcgcc agcgaatagc cagcgtcttt tctccctgcc gaattcgttg
     3841 tcatctcaga ccccgtgcct ctactgggtt cccctctttt caaggcactg cagaacaaag
     3901 ttgttaaaac gcttttactt actcgacgcc atgttgttgt ttcgcgtgat tatccccgtc
     3961 gcggagcgcg aatcctacac cagaatcgtg aagctgtgat ggatgtctga tcccgccgca
     4021 acgggccaaa cataacgttc gcgccgtttg acggaatcag tcagtacgcg ttcttgtcgc
     4081 gcgcgatgac ccagatggtc ttgaggatga ttttcagatc gagccacggt gaccagtggc
     4141 tgagatactc gagatcgtag cggacgcgct cgcgcatctt gtcgagcgtt tccgtctcgc
     4201 cccgcaggcc attcacctgc gcaaggccag tgatgccggg acgcaccttg tgccggatca
     4261 tgtagccgct gatcagcttg cggtacagct cgttgtgcgc cactgcgtgc ggccggggtc
     4321 cgacgacgct catctttcct tcgagcacat tgagcagctg cggcagctca tccagcgaag
     4381 tggcccgcag cagtcttcc
//
LOCUS       DASRHN010000139         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2353021, whole genome shotgun sequence.
ACCESSION   DASRHN010000139 DASRHN010000000
VERSION     DASRHN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353021"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="choX"
                     /locus_tag="VFB37_05305"
     CDS             complement(<1..966)
                     /gene="choX"
                     /locus_tag="VFB37_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015220 - choline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015871 - choline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter substrate-binding
                     protein"
                     /protein_id="HZO21903.1"
                     /translation="MRLQSVLLAAALAVLGVPSLAGAAGRSVTTDRPSCKSVRLADIG
                     WTDVSATTALFAALVGKLGYQPQITVLSVPVTFASMKNRDIDVFLGNWMPAEKTDRRP
                     YDADGSIEVLGPNLTGAKYTLAVPAYAYAAGLRDFSDIHRFAEPLKHSIYGIEPGNDG
                     NHLVLGMLKQNLFGLGDFKLIESSEQGMLAQVERAVHDHEPIVFLGWAPHPMNMRFDL
                     RYLTGGDAVFGPNFGGAIIYTTTRAGYSAACPNLGRLLRNLKFSVEGESQLMDAILNQ
                     HLAPQSAAERWMQANVPVVDQWLQGVTTYDGRPGSSALRGMRNRIG"
     gene            1218..>2961
                     /gene="betI"
                     /locus_tag="VFB37_05310"
     CDS             1218..>2961
                     /gene="betI"
                     /locus_tag="VFB37_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HZO21904.1"
                     /translation="MNKQSTPHGVARRDRERSGTVPRDELEDVRRRQLIEVTIDSLAE
                     AGYVGSTLAQIANRAKVSPGLVAHYFHDKDSLLEAAFRTLGRRLADGVRARMRGAHTP
                     RGRIQAIIDTNLGPEEFTQRTGSAWLAYWGQVLHVHGLKRVQDVYQRRMLSNLRHALK
                     RMLPAGEARSLAATIAAMIDGVWLRAALSNWQEADSESARALLTAFVDARLMERALPT
                     DPATDAAGSQPRLPVRPDTFASINPATGAVLARIKVDGPEQIEAAVRLAQSAQRKWAA
                     TSGTERGRILRRAAELLRARNDELAHLETCDTGKPIQETRVVDVVSGADCFEYFAGLA
                     ASLSGEHLDLGPQAFGYTRREPLGVVAGIGAWNYPLQIACWKSAPALACGNAMIFKPA
                     DLTPLTAMKLREVLSAAGLPEGLFQVVQGFADTGRLLTRHPLIRKVSVTGAVATGKAV
                     MADAAPTLKHLTLELGGKSPLIIFEDARLDDAVSGALLGNFYSAGEVCSNGTRVFVQH
                     RIKAAFIEKLVERVRALRIGDPLDPQTQVGALISEAHMEKVLGYVARGKAEGARVLVG
                     GYRLGTGELAHGFFV"
BASE COUNT          523 a          923 c         1019 g          496 t
ORIGIN      
        1 cccgattcgg ttgcgcatcc cgcgcagcgc gcttgagccc ggcctgccgt cgtaggtcgt
       61 gaccccctgc agccactgat ctacgaccgg cacattcgcc tgcatccacc tctcggcagc
      121 cgactgtggc gcgagatgct ggttgaggat cgcgtccatg agctgactct cgccctcgac
      181 cgagaacttc aggttgcgca gcaagcgccc caggttgggg caggccgccg agtaaccggc
      241 gcgggtggtg gtatagatga tggcgccgcc gaagttgggc ccgaacacgg catcgccgcc
      301 tgtcagataa cgcagatcga agcgcatgtt catcggatgc ggagcccacc cgagaaacac
      361 gatcggctca tggtcgtgca cggcgcgctc gacctgggcg agcattccct gctcgctcga
      421 ttcgatcagc ttgaagtccc cgagcccgaa gaggttctgc ttgagcatgc cgagcaccag
      481 gtggttgccg tcattgcccg gctcgatgcc atagatggaa tgcttcagcg gctcggcgaa
      541 gcgatgaata tccgagaagt cgcgcaagcc tgccgcatag gcgtaggccg ggacggcgag
      601 ggtgtatttc gcgcccgtga ggttgggccc caggacctcg atggagccat cggcatcgta
      661 aggcctgcgg tcggttttct cagcgggcat ccagttgccg agaaaaacgt cgatgtccct
      721 gttcttcatg gaggcgaagg tgaccgggac ggagagcacg gtgatctgcg gctggtagcc
      781 gagcttgccg acgagcgctg cgaagagggc cgtggtggcc gagacatccg tccagccaat
      841 gtcggcgagc cggacgctct tgcagcttgg ccgatccgtc gtcacggagc gcccggccgc
      901 tccggccagg gacggcacac caagcaccgc cagggcggcg gccagcagga cgctttggag
      961 tctcacggcg acccgatgcc aagcccccga gggtggcgac atgcacgctc cgatgcggtt
     1021 cggacctgac gggcttgacc cgcccgggtg gcgacgacat gatcctgcag gcattcttga
     1081 atagccgttt aacggacccg ctgtcaagcg tctcggacag ggggcaagtg cttgatgggg
     1141 cgcggggcac ggtcccgaag aacacgccgg ggggaaggca ggaccgaggc tcgtatggag
     1201 catggcggag taccgcattg aataaacaat caacgcctca cggggtcgct cgccgggacc
     1261 gggagcgcag cggtacggtc ccgcgcgacg agctcgagga tgttcgccgc cgccagctga
     1321 tcgaggtcac catcgatagt ctcgcggagg cgggctatgt cggcagcacc ctcgcgcaga
     1381 tcgcgaaccg ggccaaggtc tcgccgggtc tggtggcgca ttacttccac gacaaggaca
     1441 gcctgctcga ggcggcgttc cgtacgctcg gtcggcgcct ggcggacggt gtgcgcgccc
     1501 gcatgcgagg cgctcacacc ccgcgcgggc gtatccaggc catcatcgat accaacctcg
     1561 gtccggagga attcactcaa cgcaccggaa gcgcatggct cgcttactgg ggccaggtgc
     1621 tgcatgtaca tggtttgaaa cgtgtgcagg atgtctacca gcgccgcatg ttgtcgaacc
     1681 tgcggcatgc actcaagcgc atgttacctg caggcgaggc acgcagcctc gcagcgacga
     1741 ttgcagccat gattgacgga gtctggttgc gagcggcgct gtcgaattgg caggaagcgg
     1801 acagcgagag cgctcgagcg ctgctgaccg ccttcgtgga tgcgcgattg atggagcgcg
     1861 ccctgccgac ggatcctgca acggacgcag cagggtcgca gccgcggctc ccagtcaggc
     1921 cggacacctt cgccagcatc aaccccgcaa ccggtgcggt tcttgcccgc atcaaggtgg
     1981 acggccctga gcagatcgag gccgcggtac ggctcgctca gagtgcgcag cggaaatggg
     2041 ccgcgacgag cggcaccgag cggggccgga tcctgcgacg ggcggcagag ctgctgcgcg
     2101 ctcgaaacga tgagctggct catctggaaa cctgtgacac gggcaagccg atccaggaaa
     2161 ccagggtcgt cgatgtggtc tcgggcgcgg actgctttga atacttcgca ggcctcgccg
     2221 cctccctctc cggagagcac ctggatctcg ggccgcaggc gttcggttac acacgaaggg
     2281 agccgctcgg ggtcgtggcc ggcatcggtg cgtggaatta cccgctgcag atcgcctgct
     2341 ggaagagcgc accggcgctg gcctgcggca atgccatgat cttcaaaccc gcggatctga
     2401 cgccgctcac cgcaatgaag ctgcgcgaag tgctcagcgc ggcagggctg ccggagggcc
     2461 tgtttcaggt ggtgcagggc ttcgcggaca ccggccgcct tctcacccgt catccgttga
     2521 tccgcaaggt gtcggtgacc ggagcggtgg ccaccggcaa agcggtgatg gcggatgccg
     2581 cacccaccct caagcatctg acgctggagc tcggtggcaa gtcgccactg atcatctttg
     2641 aggacgcgcg cctcgacgat gcagtatccg gtgccttgct cggcaacttc tactccgcgg
     2701 gcgaggtgtg ctcgaacggt acgcgtgtgt tcgttcagca ccgcatcaag gctgcattca
     2761 tcgaaaagct cgtggagcgg gtgagggcgc tgcgcatcgg cgatccgctc gatccacaga
     2821 cacaggtcgg cgcgctgatc tccgaggctc acatggagaa ggttctcggt tacgtcgcgc
     2881 gcggcaaggc agagggcgcg cgggtgctgg tcggaggcta tcggctggga accggtgagc
     2941 tggcgcatgg cttcttcgtt g
//
LOCUS       DASRHN010000140        13799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1694631, whole genome shotgun sequence.
ACCESSION   DASRHN010000140 DASRHN010000000
VERSION     DASRHN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694631"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFB37_05315"
     CDS             complement(<1..771)
                     /locus_tag="VFB37_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZO21905.1"
                     /translation="MDAASWLWVAIAAGVLAVLYGVFSMSSILRLPPGNPRMQEIAAA
                     IQAGAKAYLNRQYTTIFIVGIVLLIALGLAIDWPTAGGFAIGAFLSGLTGYIGMNISV
                     RANVRTAQAATLGLNAALRVAFRGGAITGMLVVGLGLLGVAGYFAILRPVIGEDGALH
                     ALIGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGD
                     NVGDCAGMAADLFETYAVTIVATMLLGGLLFASRGEGAT"
     gene            complement(880..2151)
                     /locus_tag="VFB37_05320"
     CDS             complement(880..2151)
                     /locus_tag="VFB37_05320"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZO21906.1"
                     /translation="MTKPLKKNAFYAQSGGVSAVINASACGVIDAARRHSRHIGRLYA
                     GRHGIIGALTEDMIDVYRENPATLRGLKYTPAGAFGSARFKLKGLEQNRAEYERLIEV
                     FRAHDIGYFFYNGGNDSMDTALKVSQIGAAMGFPITCVGIPKTVDNDLPHTDCCPGFG
                     SVAKYVAISTREAGLDVASMARTSTKVFVLEVMGRHAGWIAAAAGLAGHSPAEPPHLI
                     LFPEVAFDRERFLSRVKQCVGDYGYCVVVVSEGAAYADGKFLADAGTRDAFGHTQLGG
                     VGPLVANMVREAHGYKYHWAVADYLQRSARHIASKVDVDQAYAVGKAAVDLAVKGINA
                     VMPTIVRKRAKPYKWVIGHIPLEQVANKEKKLPPDYIASDGFGITAACRRYLEPLAGG
                     EAYPPYKDGLPAYVRIKGVTVRRKLKTEFPV"
     gene            2259..2969
                     /locus_tag="VFB37_05325"
     CDS             2259..2969
                     /locus_tag="VFB37_05325"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZO21907.1"
                     /translation="MRIVLLGAPGSGKGTQSQRLVKLHSIPQISTGDLLRAAVVKGSP
                     LGLKAKKAMDAGALVDDQIVLGMIRERLAEADVRNGFVLDGFPRNLAQAQALEQLLGE
                     LAQPLDAVIQMEVEYAELMRRISGRRTCADCGRVFNVAEPASSPGCGEACPKTGAPHQ
                     LFQRPDDNEQTVAERLRVYEDKTRPLVEFYRAKGILRTIDAQGQVDAVTQRLEQVLRD
                     SASAALPKSRGASQPRRG"
     gene            complement(2970..3833)
                     /locus_tag="VFB37_05330"
     CDS             complement(2970..3833)
                     /locus_tag="VFB37_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO21908.1"
                     /translation="MNESITELRAMLQLEREARTESERLGRLKDEFLEALGQELRAPL
                     NSIIGWSRLMELGRLDAAETRSGGETIARNARQLAHLVDDLLDLSAITSGKIRVDPEE
                     TDICEIVEAALDMLSPMAKGKGVQLRKEFATPECQVFGDPHRLQQVIWHLLSNAIKFT
                     ARDGLALVSVKSDGAHCEVSVTDTGTGIPSEQLPFVFDRFWRADTANAQGYPGLGLGL
                     ALAKRLVELHGGTVSASSAGCNQGSRFTVVLPRVAPRIVGTEPGPTSQPFDTSVRPEE
                     TALRGEAPRTD"
     gene            complement(3903..5009)
                     /gene="rnd"
                     /locus_tag="VFB37_05335"
     CDS             complement(3903..5009)
                     /gene="rnd"
                     /locus_tag="VFB37_05335"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HZO21909.1"
                     /translation="MYRSSPKQLQPLITTSAALIELVGRLRAATSVGLDTEFLRERTY
                     RAELCLLQLSSPDDATCVDPLAVTDLAPLAPMLVAAHTTKVMHASRQDVEVLFPIAGL
                     VQPLFDTQTAAALIGLPAQVGYAELVRRLLGRELAKAHTRTDWSRRPLSAEQIEYALD
                     DVRYLLPLQAQLVEKLERSGRLAWLEEEMQALTDSRHITAEPEQAWQRVKGLRSLDPA
                     RERLAQRLAAWRERRAIERNRPRGWILEDARLRDIVMQVPRSAAALAALPEFPAAVLR
                     HCGSELLECIRDAQVPDPAPPINTRMRPDPLKSALIRKLALLNQSIAAELGISPEVLA
                     TRRDLELLADGRRDLTLLRGWRRRVLGERMLAAL"
     gene            5055..5573
                     /locus_tag="VFB37_05340"
     CDS             5055..5573
                     /locus_tag="VFB37_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21910.1"
                     /translation="MKDLIRLFAQIALLRRGPQDVPASPLLLALTIAGYFLVNLLVSW
                     VLPPFQGPWLAHLGVDVGFMFVWYALLMRLVRRPERFLQTTTAVYGYQAVLAPLLVAS
                     ESLTRRFAQDAFWQLPVTLMSLALLVWIIAANSHIVKAALEWSMPPSVALVILQTLAG
                     YLLLVALFPIPP"
     gene            5605..6987
                     /gene="mpl"
                     /locus_tag="VFB37_05345"
     CDS             5605..6987
                     /gene="mpl"
                     /locus_tag="VFB37_05345"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZO21911.1"
                     /translation="MHVHILGICGTFMGGVAAIARAAGHRVTGSDRNVYPPMSTQLEA
                     LGIEVTEGFEASQLEPAPDVIVVGNVMTRGQPVIEALLERRIPYMSGPEWLAREVLRD
                     RWVLAVAGTHGKTTTSSLVAWILEDAGLAPGFLIGGVPANFGCSARLGEAPFFVIEAD
                     EYDTAFFDKRAKFVHYHARTVVLNNLEYDHADIYPDVESIQRQFHHLVRIVPGSGLIV
                     WNAADARLQQTLEMGSWTPLEGFARSGAQDVRATWTARPAEGAEDYSRFDVFEGGRLQ
                     GTVEWSLIGAHNMENALAALAAARHAGIAVQQSIASLRSFKGIARRMQLRGEINGVRV
                     YDDFAHHPTAIATTVDGLRRRVGKARIIAVLEPRSNTMRMGVHQQTLAPSLERADEVW
                     LYTGADLGWDASSVIAELGSRAHTGKEVDTLARELARSVRPGDHVLIMSNGGFGGLHG
                     KLLAELTHMR"
     gene            7157..7783
                     /locus_tag="VFB37_05350"
     CDS             7157..7783
                     /locus_tag="VFB37_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZO21912.1"
                     /translation="MSRTTQSGPSAATSAGGTPLFPLNTVLFPGGPLPLRIFETRYLD
                     MVSHCIRRRRPFGVLLLREGSEVGAGGAGQTSELGTMARIMDFDSLPDGLLGITCRGQ
                     RKFSVLRRWQQPDGLHLGDIEFCAPEERIELPGEFQHLGELLRRVLPELGDLYAEVPK
                     HFNDASWVGCRLAEILPLSLTEKQYCLELQDPLARLARLSPLIRRAEE"
     gene            complement(7968..8642)
                     /locus_tag="VFB37_05355"
                     /pseudo
     CDS             complement(7968..8642)
                     /locus_tag="VFB37_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
     gene            8806..9213
                     /locus_tag="VFB37_05360"
     CDS             8806..9213
                     /locus_tag="VFB37_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21913.1"
                     /translation="MQEQRARKILQALVQGLDPVTGEELPSGTLLQQADVLRALLVGV
                     AALDQVAARAQRRSQLPENVGRAWTPEEEQSLIVAFESGEALAEVAARHHRTLRAIEA
                     RLERLGLLAAEQRITNNSFTGSPTVRATDPRPP"
     gene            complement(9167..10234)
                     /locus_tag="VFB37_05365"
     CDS             complement(9167..10234)
                     /locus_tag="VFB37_05365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO21914.1"
                     /translation="MSSVLFFDPSCQRPYDTRTLREQATGGTEASVTRVADALGAFVM
                     QHNRTEAFESYLPPGRIAGIEHVIVNRDSRAFSQVRRLYPHARMYLWVHDQLNPGSKR
                     GRRLASTAPELRELGVRVVCVSDTQRAGVEATLRHIRVADVVSARTIYNPVEDELAPD
                     GSSIDDRKLVFFSSPNKGLALALDAFRALHRRMPDLRLQVGNPGYKIRRSAPIAGVEY
                     LGPQPQQRIHLEVRTALCTFFPNFVLPETFGLVFAESMALGTPVLTHDCGAAAEIIAD
                     PQQILPVTFALRAYEKAFSGWSPGLRRSPAQIADRLGLFDAYVEKIRAWRNGARPGTG
                     PDPRFRLTAVVDRWRALLESP"
     gene            10374..11153
                     /locus_tag="VFB37_05370"
     CDS             10374..11153
                     /locus_tag="VFB37_05370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO21915.1"
                     /translation="MRLETSGRGRLSACIITYNEADRIEACLRSVAFCDEILVVDAHS
                     TDATREIAVSLGARVIERDWPGYRSQKQFAVDSATSDWVLCLDADERVSAGLRAEVES
                     LREAGFAGYAGWSVPRITDYFGRFLRHGNAFPDRLVRLFDRRRGGWVGYEIHENTQVS
                     GRTGRLRGYLEHYSYRSLSDHQERMRRYADLMARALHERGKRGSLLKVLVNPQWRFLR
                     GYFLRLGFLDGWRGLVFALIEANYVRRKYLQLYMLSRGLPA"
     gene            complement(11169..12038)
                     /locus_tag="VFB37_05375"
     CDS             complement(11169..12038)
                     /locus_tag="VFB37_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HZO21916.1"
                     /translation="MGRKTRRWLFALGLLGSLSAHAGSPVWAIHGDHNTVYLAGSVHL
                     LKAGDAALPPAFNRAYSSSKSLVMELALDKVDPMEAAGWMMEHGTLKEGTDLREVIGD
                     ERYKRMSAEAERLNVPTEVLDRLEPWVVALQLLELKYVQLGFDPQQGVEQQLEHRAQA
                     DGKPIEGLETLDEQLGVLQGMSYSDQARFLDMVIGEMQDVGSETDSVVDAWRRGDSAK
                     LGTLLSDEYKSFPALYRALVTDRNRHWLPQIEKMLHADSDYLVVVGALHLVGQGGLLE
                     LVQHDGYKPEAVN"
     gene            complement(11984..12778)
                     /locus_tag="VFB37_05380"
     CDS             complement(11984..12778)
                     /locus_tag="VFB37_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HZO21917.1"
                     /translation="MSVLRLIQFTDPHLYGNELEMLRGIATLPALKAALADAQSSTRW
                     PPNAVLVTGDIVQDDPGGYAHLRRVLGSLGLPVLCIPGNHDEPQAMRRELAGEPFVLD
                     AVVDFSHWRIVQLDSSVPGSASGRLSPQSLRQLETALQSAQGRHCLVCLHHHPVPMSS
                     RWLDQVGLENATEFLATLDRHRNVRAVLWGHVHQAYDGLRKDVRLLGTPSTCAQFLPH
                     ADDFALDRRPPAYRTLELRADGSVLTEVMWVEKRAAGSSRSACSAA"
     gene            13147..>13799
                     /locus_tag="VFB37_05385"
     CDS             13147..>13799
                     /locus_tag="VFB37_05385"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZO21918.1"
                     /translation="MTTRRELANCIRALAMDAVQRANAGHPGAPMGMADIAQVLWGDF
                     LKHNPGNPLWVDRDRFILSNGHASMLLYALLYLTGYPLGLEEIKNFRQLGSRCAGHPE
                     HEPAIGIETTTGPLGQGLANAVGLALAQKLLAAHFNKPGFNIVDHYTYVFAGDGCLME
                     GISHEACSLAGTLQLGKLIGFYDNNGISIDGKVEGWFTDDTNKRFEAYGWHVEPEVDG
                     "
BASE COUNT         2467 a         4441 c         4496 g         2395 t
ORIGIN      
        1 tgttgcgccc tctccccggc tggcaaacag caggccgccc aggagcatag tggcaacgat
       61 cgtcaccgcg tacgtctcga acaggtcagc agccattccc gcacagtcgc caacattgtc
      121 accgacgttg tcggcgatca cagcggggtt gcgcggatcg tcctcgggaa tgcccgcttc
      181 caccttgcct acgagatctg cgcccacgtc ggcgcccttc gtgaagatgc caccgcccag
      241 acgagcgaag atcgagatca gagagccgcc gaaggcgagc ccaatcaaag cgtgcagcgc
      301 tccgtcctcg ccgatgacgg gccggaggat ggcgaagtaa cctgcaaccc cgagcagtcc
      361 aagaccgacg accagcatgc cggtaatggc gccgccgcga aatgcgacgc gcagtgccgc
      421 gttgagtccg agcgtggccg cctgggcggt gcgcacattc gcgcgcaccg aaatattcat
      481 gccgatataa ccggtaaggc cggagagaaa ggcgccgatg gcgaagcctc cggctgtcgg
      541 ccaatcgatg gcgagcccca gggcaatgag aagcacgatg ccgacgatga agatggtcgt
      601 gtactgtcga ttgaggtagg ccttggcgcc ggcctgtatc gccgctgcga tctcctgcat
      661 gcgcgggttg cccggaggca ggcgcaggat cgagctcatc gagaacacgc cgtagaggac
      721 ggcgagcacg ccggcagcaa tcgccaccca cagccacgaa gcggcatcca tcttcagttc
      781 tccccaagga tcaatgagtt gtaatcccaa aaaggtcgcg cactatatca cgccaggccc
      841 tgccggcagc cgcccgcagc ggcgtgggat cggttgcgat caaaccggga attcggtctt
      901 gagcttgcgc cggacagtga cgcccttgat gcggacataa gccggcaaac catccttgta
      961 cggagggtag gcttcacccc cggcgagcgg ttcgagatag cgacgacagg cagcggtgat
     1021 gccgaagccg tcggaggcga tgtagtccgg cggcagcttc ttctccttgt tcgccacctg
     1081 ctccagtgga atgtgtccga tgacccactt gtagggtttg gcgcgcttgc gcacgatcgt
     1141 gggcatcacg gcgttgatac ccttcaccgc caggtccacg gccgccttgc ccaccgcgta
     1201 cgcctgatcg acatcgacct tggaggcgat gtggcgtgct gaacgctgca ggtaatcggc
     1261 aaccgcccag tgatacttgt agccatgagc ctcgcggacc atgttcgcaa cgagcggccc
     1321 gaccccaccg agctgcgtgt gaccgaatgc gtcccgggtg cccgcatcag ccagaaactt
     1381 gccatcggcg taggcggcgc cttcggagac gacaacgaca caatatccgt agtcgcccac
     1441 gcactgcttc actcgcgaaa ggaatcgctc gcgatcgaag gcaacttcgg gaaacaggat
     1501 cagatgcggg ggctcggccg gggagtggcc tgcaagcccg gctgccgccg cgatccagcc
     1561 cgcgtggcgg cccatgacct ccagaacgaa caccttggtg gaggttcggg ccatggaggc
     1621 gacatcaagg cccgcctcgc gggtagagat cgcgacatat ttcgccaccg agccgaaccc
     1681 cgggcagcaa tcggtgtgcg gcagatcgtt gtccaccgtc ttgggaatcc ccacacaggt
     1741 gatcggaaag cccatggccg cgccgatctg cgagaccttg agcgcggtgt ccatcgagtc
     1801 gttgccgccg ttgtagaaga aatagccgat gtcgtgcgcc cggaagacct cgatcaaccg
     1861 ttcgtactcg gcgcggttct gctccaaccc cttcagtttg aaacgggccg atccgaaagc
     1921 ccccgccggg gtgtacttca gcccgcgcag cgtcgcgggg ttttcccggt acacatcgat
     1981 catgtcctcg gtgagtgcgc cgatgattcc atggcggccc gcatagagcc ggccaatgtg
     2041 ccgggaatgc ctgcgggcag cgtcgatgac gccacaggcc gatgcgttga tcacggcgga
     2101 aaccccgcct gactgggcgt agaaagcgtt cttcttcaac ggtttcgtca tgatgttggc
     2161 gcaggggtga gcggggggcg caaggatagc gcagtgaggc gaactccggt aacgtggcga
     2221 gcgtttcacg gatacaggac ctcgacgtac agcacatcat gcgcatcgtt ctgttgggcg
     2281 cgcccggttc gggcaagggg acgcaatcgc agcgtctggt caaacttcac tccatcccgc
     2341 agatctccac cggcgatctt ctgcgcgcgg ctgtcgtcaa gggcagccct ctcggcctga
     2401 aggcgaagaa ggcaatggat gccggcgcac tcgtggacga tcagatcgtg ctcggcatga
     2461 tccgcgagcg gcttgccgag gcggatgtgc gcaatggctt cgtcctggat ggctttccgc
     2521 gcaatctggc ccaggcacag gcgctcgagc agctgctcgg cgagctcgca cagccgctgg
     2581 atgccgtcat ccagatggag gtggagtatg cggagctcat gcggcgcatc tccggacggc
     2641 gcacatgcgc cgattgcggg cgcgtgttca atgtagcgga gccagcttcc agcccgggct
     2701 gcggggaagc ctgtccgaag accggcgccc cgcaccaact ctttcagcgg ccggacgaca
     2761 atgagcaaac cgtggccgag cgcctccggg tctacgagga caagacgcgt ccgctcgtag
     2821 agttttatcg cgcgaaggga atcctgcgga caatcgatgc gcagggccag gtcgatgccg
     2881 tgacgcagag gctggagcag gttctacgcg attccgcctc ggccgcactg ccgaagagca
     2941 gaggcgcgag ccagccgcgg cggggctgat cagtccgttc gcggcgcctc gccgcgcagt
     3001 gccgtctcct cggggcgcac ggaggtgtcg aaaggctggc tcgtcggccc cggctctgta
     3061 ccgacaatcc ggggcgccac gcgcggcaga accaccgtga accgcgatcc ctgattgcat
     3121 ccggcactgc tggcgctgac cgtaccgccg tgcagctcga ccagccgttt cgccagggcg
     3181 aggccgagtc cgagtcccgg atagccttgc gcattggccg tatccgcgcg ccagaatcga
     3241 tcgaagacaa aggggagttg ctcgctgggg atacccgtcc cggtgtccgt gacggagact
     3301 tcgcaatgag caccgtcgga tttgacgctg acgagcgcca ggccatcgcg ggcggtgaac
     3361 ttgatggcat tgctcagaag gtgccagatg acctgctgca agcgatgcgg gtcgccgaac
     3421 acctggcact cgggggtcgc gaattcctta cgcagctgaa ctcccttgcc cttggccatg
     3481 ggcgaaagca tgtccagcgc cgcctcgacg atctcgcaga tgtcggtctc ctcgggatcg
     3541 acgcggatct tgccgctcgt gatcgcgctc aagtccagaa gatcgtccac gaggtgcgca
     3601 agctggcgcg cgttacgcgc tatggtctcg ccgcccgaac gggtctcggc ggcatccagc
     3661 cgtccgagct ccatcagccg tgaccagccg atgatcgaat tcaggggcgc tcgcagctcc
     3721 tggccgagcg cctcgaggaa ctcgtccttc agccgtccca gccgttcgga ttcggtgcgc
     3781 gcctccctct cgagctggag cattgcgcgc agctccgtga tcgactcatt catggcctgt
     3841 cagcaccgca gtggtctcga gtttggccgc ctcatgttcg acccacttta taatccattc
     3901 gttcatagtg cggccagcat ccgttccccc aggacacggc gccgccagcc acgcagcagg
     3961 gtgagatcgc gtcgcccgtc tgcgagcagc tccaggtcgc gccgggtggc caacacttcc
     4021 gggctgatgc cgagctcagc cgcgatgctc tgattgagca gtgcgagttt cctgatgagt
     4081 gcgctcttga gcggatcggg ccgcatccgc gtgttgatcg gcggggccgg atccggcact
     4141 tgcgcatcgc ggatgcattc gagcagctcc gagccacagt gcctcaggac ggcggcgggg
     4201 aactccggaa gtgcggccag cgcagcggcc gagcgcggca cctgcatcac gatgtcgcgc
     4261 aagcgtgcgt cttccaggat ccagccgcgc gggcgattgc gctcgatggc tcgccgctcg
     4321 cgccaggcgg ccagccgctg cgccaggcgc tcgcgcgccg gatcgagcga gcgcagccct
     4381 ttcacgcgct gccacgcctg ctccggctcg gcagtgatat gtcgactgtc ggtcaatgcc
     4441 tgcatttcct cctcgagcca ggcaaggcgg ccgctacgct cgagcttttc cactaactgt
     4501 gcctgcaacg gcaggagata gcgcacatca tccagagcgt actcgatctg ctccgccgat
     4561 aaagggcgcc gcgaccagtc ggtccgggtg tgcgcctttg caagctcccg gcccagcaga
     4621 cggcgtacga gctctgcata tccgacctgg gccggcagtc caatgagcgc cgcagcggtc
     4681 tgcgtgtcga acagcggctg cacgagtcca gcgatcggaa acagtacctc gacatcctgt
     4741 cgtgaggcgt gcatgacctt ggtggtgtgc gcggcgacga gcatcggtgc gagcggcgca
     4801 agatcggtga ccgccagcgg atcgacgcag gtcgcatcgt cgggtgagga caattgcaac
     4861 aggcaaagct cggcccggta cgtgcgctct ctgaggaatt ctgtgtccag ccccacactc
     4921 gtggcggccc gcagtcggcc gacgagctcg atcagcgctg cggatgtcgt gatgaggggc
     4981 tgcaactgtt tcggggatga tctgtacaat cgggcgatgc ccattatgcg agtcggacgt
     5041 ccgtatcgaa agccatgaag gacctgatcc gactgttcgc gcagatcgcg ctgctgcgcc
     5101 gcgggcctca ggacgtgccc gcatcgccgc tgttgctggc attgacgatc gcgggttatt
     5161 tcctcgtcaa tcttctcgtg agctgggtat tgccgccatt tcagggaccg tggctggcgc
     5221 atctcggtgt ggatgtcggc ttcatgttcg tctggtatgc actcctgatg cgactcgttc
     5281 gccggccgga gcgcttcctg cagaccacca ccgccgtgta cggctaccag gcggttctcg
     5341 cgcccttgtt ggtcgcgtcg gagtcgctga cgcgccgctt cgcgcaggat gcgttctggc
     5401 agctgcccgt gaccctgatg agcctcgctc tgctggtctg gatcatcgcc gcgaacagcc
     5461 atatcgtgaa ggcggcactc gaatggtcga tgccgcccag cgtcgcgctc gtcatcctgc
     5521 agaccttggc gggctatctg ctgctggtcg ccctgtttcc cattccaccc tgacagcgtc
     5581 atcgcaactg gagcgatcag gtccatgcac gtccacatac tcggcatttg cggcaccttc
     5641 atgggcggcg tagccgcgat cgcgcgcgcg gcgggtcacc gcgtgacggg ctctgaccgc
     5701 aatgtctatc cgccgatgag cacgcagctc gaggcgctgg gtatcgaagt gacggagggc
     5761 ttcgaggcct cgcagctcga gccggccccc gatgtcatcg tggtcggaaa cgtgatgacc
     5821 cggggccaac cggtcatcga agcgctgctc gagcgccgca ttccgtacat gtccggaccc
     5881 gaatggctcg ctcgcgaggt gctcagggac cggtgggtgc tggccgtggc cggaactcac
     5941 gggaagacga cgacctcctc gttggtggcg tggatcctcg aggatgcggg acttgcgccc
     6001 gggtttctca taggtggcgt acccgccaac ttcggttgca gcgcccgtct gggcgaggcg
     6061 ccgttcttcg tcatcgaagc cgatgaatac gacacggcct tcttcgacaa gcgcgccaag
     6121 ttcgttcact accacgcccg cacggtggtg ctcaacaacc tcgaatacga tcacgctgac
     6181 atttatcctg atgttgagtc gattcagagg cagttccacc atctcgtgcg catcgtcccc
     6241 ggcagcggcc tcattgtctg gaatgccgcc gacgcgcgac tgcagcaaac cctggagatg
     6301 ggcagctgga cgccgctcga ggggttcgcg cgctcgggtg cgcaggacgt gcgcgccacg
     6361 tggaccgcgc gccccgcaga gggggcagaa gactattcgc gcttcgatgt attcgagggc
     6421 ggacggttgc agggtacggt ggagtggtcg ctgatcggtg cccacaacat ggaaaacgcg
     6481 ctcgccgccc tcgcggcggc ccggcatgcg ggcatcgcag tgcagcaatc aatcgcctcg
     6541 ctgcgctcgt tcaaggggat cgcacggcgc atgcagctgc gcggcgagat caacggagtc
     6601 cgtgtgtacg atgacttcgc acatcacccg acggcgatcg ccacgaccgt ggacggattg
     6661 cggcggcgcg tcggcaaggc ccggatcatc gcggtgttgg aaccccgctc caacacgatg
     6721 cgcatgggcg ttcaccagca gaccctcgct ccctcgctgg aaagggcgga tgaagtctgg
     6781 ctgtatacgg gcgcggatct gggctgggat gcttccagcg tcattgccga gctgggctcg
     6841 cgtgcccaca ccggcaagga agtcgacact ctggcgcgag aactggcccg ctccgtccgc
     6901 ccaggggatc acgtgttgat catgagcaac ggaggcttcg gcggcctgca cggcaagctc
     6961 ctcgccgagc tgacgcatat gcgctagggc ctgttcacac ttgcctggct gccgcacagg
     7021 gtgcgaaagt cgcgcctttc gcacccggca tcggagctca tgtctgaaaa atcgctgcag
     7081 cgatttttcc catggactag gctgcgttgg acgggaggcg gctacgacag gtgcctctcc
     7141 cggatagccg ccggcgatgt cccgcaccac acaatccgga ccctccgctg ccacctctgc
     7201 aggcggaaca ccgctgtttc cactcaacac cgtgttgttt cccggaggtc ccctgccgct
     7261 gcggatcttc gagacgcgct atctggacat ggtgagtcat tgcatccgca gacggcggcc
     7321 gttcggggtg cttctgcttc gtgagggatc ggaggtgggc gcaggcgggg ccgggcagac
     7381 gagcgagctc ggaacgatgg cgcgcatcat ggacttcgac tcgctgcccg acgggcttct
     7441 gggtatcacc tgccgcggcc agcgcaagtt cagcgtcctg cgtcgctggc agcagcctga
     7501 tggattgcat ctgggggaca tcgagttctg cgcgcccgag gagcgcatcg agctgcccgg
     7561 cgagtttcag catctcggcg agttgctgcg cagagttctg cccgagctgg gcgacctcta
     7621 cgccgaggtg ccgaagcact tcaacgatgc ctcctgggtg ggttgtcggt tggctgagat
     7681 cctgccgttg agtctgacgg agaagcagta ctgtctcgag ctgcaggatc ccctcgcgcg
     7741 cctggcgagg ttgagtccct tgatccggcg ggcggaagaa tgagccgggg tgccgctgcc
     7801 actgcgtggc gccggtagaa gctcagcagc gcaaatccca gcaccaccgt ccaccccagc
     7861 acgagccagc ccacgaaccc atcgaattgg ctgagcagaa agctcagtgg ctgccccctg
     7921 acaggtcgcg tcagctcgtg cgccaccagc atgacccaga cccaaccggc atgcagcccg
     7981 atgcacgcgg cgatgttgcc tgtgaccgcc cggatgcagg cgagcaccac gccaacggcg
     8041 aacaggcaca gaaaagcatc ggcaatctgc agcggatctg caaacaggtg cagcgtgcca
     8101 tccaacagcg cgagcccgct ggcggcgctc acctgttgcg cgggaatgtg aaagctggcg
     8161 aaaaagtgtg tggccgagta gacgatggaa gtcaggatga tggcggccgg cgcgccggac
     8221 tcgcgctcga ttccggtgaa cattgcgccg cgcaggaacg tctcctcgat gagaccgacc
     8281 gcaaggccgc tcaacagtcg ggctccgatc aggctggcga ggcgagcgcc gctgatccct
     8341 tgcgcctcac tccagttgag caggcccagc cccgtcatga ttgccacgat gagcagcatg
     8401 gtgacggcgc cgatggccag tgcgcgcagc agctcgcgca caaacaggcg gcgcgggagc
     8461 ccatatccca ggctgcatcg gtcgccgagc ttcgcccggc gcgcgatcag gacgaacccg
     8521 accagcaggg ccagcatgcc gatccgctct ccgatgcgat ggaacggaaa atcgaagtgc
     8581 ggatgcagca gcagccaggc ggggtaggaa aacactgcca tcgccgcgag cgcgagcagc
     8641 atcaggccca ggaagagcgc gaacgcgcgc actcagcagc ctccatttgc agacccgccg
     8701 cagcgcgtgc gcgtaaatct gcgtaaacac gcagtctttc gcgctggccc gcggtgacgt
     8761 tccactcgga caattgcgcg ctcagtcatc acggagggat gggtcatgca ggaacaacgt
     8821 gcacgcaaga tacttcaggc gctcgttcag gggctggacc ccgtgacggg cgaggagctt
     8881 cccagtggaa ccctcctgca gcaggcggac gtgctgcgcg cactgctggt aggcgttgcg
     8941 gccttggatc aggttgcggc tcgtgcgcag aggcgctcgc agcttcctga gaatgttgga
     9001 cgcgcctgga cccccgagga ggagcagagc ctgatcgtcg cattcgaatc cggagaggcg
     9061 ctggcggagg tggcagcgcg tcatcaccgc acgctccgcg caatcgaggc gcgactggag
     9121 cggctcggtc tgctggccgc cgagcagcgc atcacgaata acagtttcac gggctctcca
     9181 acagtgcgcg ccaccgatcc acgaccgccg tgaggcgaaa gcgcgggtcg ggccccgtgc
     9241 cgggccgcgc accgttgcgc caggcgcgga ttttctcgac gtaggcgtcg aacagtccga
     9301 gccgatctgc gatctgtgcc ggagagcggc gcaagcccgg tgaccagccc gagaacgctt
     9361 tctcataggc gcgcagcgcg aaagtcacgg gcaggatctg ctgcgggtcg gcaatgatct
     9421 ccgccgccgc gccacagtca tgtgtcagga ccggcgtgcc gagggccatc gactcggcga
     9481 acaccagtcc gaaggtctcc ggcagcacga agttaggaaa aaaggtgcac agcgccgtgc
     9541 gcacctccag gtgaatgcgc tgctgcggct gcggaccgag atattcaacg cctgcaatcg
     9601 gcgcggagcg gcggatcttg taaccgggat tgccgacctg cagccgcagg tccggcatgc
     9661 ggcggtgcag agcccgaaag gcatccagtg ccagggccag acccttgttg ggtgaggaga
     9721 agaaaaccag cttgcggtcg tcgatcgatg agccatccgg agcgagttca tcctccacag
     9781 ggttgtagat cgttctcgcg ctgaccacgt ccgccacgcg gatgtgacgc agggtcgcct
     9841 cgacacccgc gcgctgcgta tccgagacgc agacgacgcg gacacccagc tcgcgaagct
     9901 cgggagcggt cgaggccagc ctgcgtccgc gcttcgagcc ggggttgagc tgatcgtgca
     9961 cccacaggta catccgcgca tgcggataga gccttcgcac ctgcgagaat gcgcgcgagt
    10021 cgcgattgac aatgacgtgc tcgattcctg cgatccggcc cggcgggagg tatgactcga
    10081 aggcttccgt gcggttgtgc tgcatcacaa acgcgccaag cgcgtccgcc acgcgagtta
    10141 cactggcttc ggtcccgccc gtcgcttgtt cccgtaacgt acgtgtgtca tacggacgct
    10201 ggcacgaggg atcgaagaac aacacggagc tcattcgcgc tattgtatta gattggaaaa
    10261 agccgccgac aggcctcccc acgaaatcga gctagcctcg tgcaacgggt ttgcagattg
    10321 cgccaagatg ctcgctttcg tgggaccccg gggcgctttt tctgatcatc gaggtgcgct
    10381 tggaaacgtc cggtcgcggc cgcctgtccg cttgcatcat cacctacaac gaggccgaca
    10441 ggatcgaggc ctgcctgcgg tcggtcgcct tctgcgacga gatcctggtc gtggatgcgc
    10501 attcgacgga cgccacccgc gagatcgcgg tgtcgctcgg tgcgcgagtc atcgagcggg
    10561 attggcccgg ctatcgctcg cagaagcagt tcgccgtgga cagcgccacc tcggactggg
    10621 tgttgtgcct ggatgccgac gagcgggtca gtgcgggctt gcgtgcggag gtcgagagcc
    10681 tgcgcgaggc gggcttcgcg ggctatgcgg gctggtcggt gccgcgcatc acggactact
    10741 tcggacgctt cctgcgccat ggcaatgcct ttccggatcg actggtgcgg ctgttcgacc
    10801 ggcgccgggg cggctgggtc ggttacgaga tccacgagaa cacgcaggtg tccggacgca
    10861 ccggccgcct gcgagggtac ctggaacatt actcgtatcg cagtctgagc gatcaccagg
    10921 aacggatgcg ccgttacgcg gacctcatgg cgcgggcgct ccacgagcgc ggcaagcgcg
    10981 gcagcctgct gaaggtgctc gtgaacccgc agtggcgctt cctgcgcggt tacttcctca
    11041 ggctgggctt tctggacggt tggcgcgggc tggtgtttgc gctgatcgaa gcgaactacg
    11101 tgcgccgcaa gtatctgcag ctctacatgc tcagccgtgg gctgccggcg taggccctcg
    11161 ggccctgttc agttgaccgc ttcgggcttg tagccgtcgt gttgcacgag ctcgagcagg
    11221 cccccctgtc cgaccagatg cagagcaccg acgacgacga gatagtcgct gtccgcgtgc
    11281 aacatcttct cgatctgcgg tagccagtgc cggttgcgat cggtgaccag cgcgcgatag
    11341 agcgccggaa agctcttgta ctcatcgctc agcagggtcc cgagcttcgc cgagtctccc
    11401 cgccgccatg catcgaccac cgaatcggtc tcgctgccga cgtcctgcat ctcgccgatg
    11461 accatgtcca gaaagcgagc ctgatcggaa tacgacatgc cctgcagcac gccgagctgc
    11521 tcatcgagtg tctcgagccc ctcgatcggc ttgccatcgg cttgcgcgcg gtgctcgagc
    11581 tgctgctcga cgccctgctg cggatcaaac ccgagctgta cgtacttcag ctcgaggagc
    11641 tgcagcgcca cgacccaggg ctcgagccga tcgagtacct ccgtcggcac attcaggcgc
    11701 tcggcttccg cgctcatgcg cttgtagcgc tcatcgccga tcacctcacg caagtcggtg
    11761 ccttccttga gcgtcccgtg ctccatcatc cagcctgccg cctccatcgg atcgaccttg
    11821 tccagggcga gctccatcac gagcgacttc gagctggagt aggcgcgatt gaacgcaggg
    11881 ggaagggcag catcgcccgc cttcaacagg tggacggatc cggcgagata aacggtattg
    11941 tggtcgccgt ggatcgccca gacggggctc ccggcgtgtg cgctcaggct gccgagcagg
    12001 ccgagcgcga agagccagcg gcgcgttttt cgacccacat gacctccgtc aacacagagc
    12061 cgtctgcgcg cagctcgagc gtgcgataag cgggcggccg cctgtccaga gcgaagtcat
    12121 ccgcgtgcgg cagaaattgt gcgcacgtcg aaggcgtccc gagcagccgg acgtccttgc
    12181 gcaatccgtc gtacgcctga tggacatggc cccacaaaac ggcacgcacg ttgcgatgcc
    12241 ggtcgagggt ggcgagaaat tccgtggcat tctccagccc gacctgatcg agccagcggc
    12301 tggacatcgg caccggatga tgatgcagac acacgaggca atggcggccc tgtgccgact
    12361 gcaatgccgt ctcgagctgc cgcagcgact gcggactcaa tcggccgctc gccgatcccg
    12421 gaacgctgct atcgagctgt acgattcgcc agtggctgaa gtccacgact gcgtccagga
    12481 cgaaaggctc gccggcgagc tcgcggcgca tcgcctgagg ctcgtcgtga ttgccgggaa
    12541 tgcacagcac cggcagaccc agcgagccga gcacgcgccg cagatgggcg tagccacccg
    12601 gatcatcctg aacgatgtcg ccagtcacca acactgcgtt cggcggccag cgcgtcgagg
    12661 attgcgcatc ggcaagcgcg gccttgagcg cagggagcgt cgcaatgccg cgcagcatct
    12721 ccagctcgtt gccgtagaga tgcggatcgg tgaactggat gaggcgcaat acgctcatga
    12781 tgagatcgcc ggctcggaat ccttgattga atgactgccg cagtgctctg gcggttccgg
    12841 agaaaggggc cggccatgcc acgaaagcgt aacagagtgc ccccgggatg atgcagtgaa
    12901 ctgggtcaaa ggcagccgcg ttctgcacct gaacttgacg ccgcgcactg ttttcgcagc
    12961 gaatattgtg aagcggaacg cgcctgtcca gcggccgcag gccgcgtagt ggctcctgct
    13021 gcgcagacgc tccgagggcg tttcgggggg tcgccgctac aggatcaccc acggaaggtg
    13081 tggcgtgaca cgcacgctgt ggcacactcg ttgttttcac cgcatgtttt tccgacgatc
    13141 gagctcatga cgacacgacg cgaactcgca aattgcattc gtgccctcgc catggacgcc
    13201 gtgcaacgcg ccaatgcggg ccatcccggc gcgcccatgg gaatggccga tatcgcccag
    13261 gtgctctggg gggacttcct caagcacaat cccggcaatc cgctctgggt ggaccgcgac
    13321 cgcttcatcc tctcgaacgg gcacgcgtcg atgctgctgt acgcgctgct gtatctgacc
    13381 ggctatccgc tcggtctcga ggagatcaag aatttccgcc agctcggctc gcgctgcgca
    13441 ggacacccgg agcacgagcc cgccatcggt atcgagacca ccaccggtcc cctcggacaa
    13501 gggctcgcca acgccgtcgg cctggcgctc gcacagaagc tgctggccgc gcacttcaac
    13561 aagcccggat tcaacatcgt cgatcattac acgtatgtgt ttgcgggcga cggttgcctg
    13621 atggagggca tctcgcacga ggcctgctcg ctcgccggca cgctccagct cggaaagttg
    13681 atcggcttct acgacaacaa cggcatttcg atcgatggca aggtcgaggg ctggttcacc
    13741 gacgatacca ataagcgctt cgaggcctat ggctggcatg tggagccgga ggtggacgg
//
LOCUS       DASRHN010000141         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1695383, whole genome shotgun sequence.
ACCESSION   DASRHN010000141 DASRHN010000000
VERSION     DASRHN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695383"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFB37_05390"
     CDS             complement(<1..475)
                     /locus_tag="VFB37_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO21919.1"
                     /translation="MSAPTERSTQQETLFTQARILETMREGVVLIDTNTHLITLTNAT
                     FDRMFGYVGHELLGQSIDPLFSVSSLQRRRFERTLRDGSEVMPVEFECSRKDGSRFVA
                     TCVVTPLRMGGSERLLAVLNDVTERKRLEREIIEIASREQQRIGSDLHDGLGQDLT"
     gene            616..831
                     /locus_tag="VFB37_05395"
     CDS             616..831
                     /locus_tag="VFB37_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21920.1"
                     /translation="MNPWRLWQPPSETIEAIAGVLRSHWAAAKSLQPEALWSGPATWL
                     RRQANRLVEPTAPIVENPEPPESELTI"
     gene            complement(821..1651)
                     /gene="hemF"
                     /locus_tag="VFB37_05400"
     CDS             complement(821..1651)
                     /gene="hemF"
                     /locus_tag="VFB37_05400"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HZO21921.1"
                     /translation="MSEPADLVDSAAAYFRDLQSRICASFEGFESTRRFEPRSWARPA
                     GHRLQGGGESRLLRGEVFEKVGVNFSHVWGEFSPQARAQVPGAEESGGRFVACGISLV
                     AHMWNPYVPAVHLNLRYLRTSRMWFGGGSDLTPTFPFSEDTAQFHTALQGACETYRPD
                     AYAQFKAWCDRYFYLPHRQEPRGVGGIFFDELSSADRQADFAFVRAVGEAFLAVYPQI
                     VARRKDTPFDDGAREKLLVKRGRYVEFNLVYDRGTKFGFSTDADPEAYLMSLPPLVRW
                     "
     gene            complement(1635..2738)
                     /gene="hemE"
                     /locus_tag="VFB37_05405"
     CDS             complement(1635..2738)
                     /gene="hemE"
                     /locus_tag="VFB37_05405"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZO21922.1"
                     /translation="MSPPRESTFLAACRRQAVAHTPIWVMRQAGRYLPEYRELRAKVD
                     FESLTRQPELAAEVTLQPLRRFELDAAILFSDIMTPVQSLGVDLTFEPAPVVRNPIRT
                     DTQIDALHELVPERDVPFVLRSIQLIRPNLPRGVPLIGFAGGPFTLLCYLVSGRPSKE
                     FDVARSFLYAQPQSAERLLDRLADAMAAYLHAQAAAGAQALMLFESWGGLLGPGAFDR
                     FALRAVRRTVAALRRSTDVPLIYYLNQGSALMERVADLDVDVIGVDWRTPLGKVRQVL
                     GPHKAVQGNLDPAALFSSPEDLRRHADEVLREAGRAAREASGDIERSGEARGSGEIRA
                     APGHIFNLGHGIWPNTDPDSVARLVDHVHERTG"
     gene            complement(2735..3712)
                     /gene="hemB"
                     /locus_tag="VFB37_05410"
     CDS             complement(2735..3712)
                     /gene="hemB"
                     /locus_tag="VFB37_05410"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZO21923.1"
                     /translation="MGFPLHRPRRTRQSDLWRRMVRETHLSLDSCIYPLFVVPGRGVR
                     HRVESMPGISQLSVDEAVAEAREVAAAGVPAVLIFAAPAQKDAKASAALDPKGLAAQA
                     ISAIKDACPQLLVWADVCLCGSTDHGHCGHVLPNGRIDNDASVESLAQVGLNYARAGA
                     DAVAPSDMMDGRVLAMRQLLDREGFETTPIVSYAAKYASSFYGPFREAAESAPAFGDR
                     RSYQMDPANAREALREVLQDVEEGADLVMVKPAGPYLDVIRQVRDAVHLPVVAYQVSG
                     EYSLIKAAAERGWIDERAAALETLTGIKRAGADLIITYFAPQLAKWLAE"
     gene            complement(3715..4506)
                     /locus_tag="VFB37_05415"
     CDS             complement(3715..4506)
                     /locus_tag="VFB37_05415"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZO21924.1"
                     /translation="MNIATPDNREVGVARETRPVVVTRAESNDGPLSTQLRTLGLSVL
                     LWPAFSVAAAKKEPLEAALKAIGQFDWIVFASRHAVAAVTARVSSPPQGVLVAAVGQA
                     TAQVLKQRGWRMELLPSEANAAALVAAFATRPIRGAKVLFPASSRALPTIATGLRQLG
                     AEVLQVEAYRTDGAPLDVEECRTWIARDGIAAVTFASPSAVIELEQALGRKDFERLLA
                     GARAVAIGPTTARALLDRGCTAVLAESATLAGLAQTTLRLLQTRH"
BASE COUNT          815 a         1602 c         1454 g          700 t
ORIGIN      
        1 cggtcagatc ctgaccgagc ccatcgtgca gatcgctgcc gatgcgctgc tgctcgcggc
       61 tcgcgatctc gatgatctcg cgctcgagcc gcttgcgctc ggtcacatcg ttgagcacgg
      121 ccagcagccg ctccgatccg cccatgcgca gcggcgtcac cacacaggtg gcgacgaacc
      181 gcgagccgtc cttgcgcgag cactcgaatt ccacgggcat cacttccgag ccatcgcgca
      241 gcgtgcgctc gaagcgccgc cgttgcagcg atgacacact gaagagcgga tcgatcgact
      301 ggccgagcag ctcgtgcccc acatagccga acatgcggtc gaacgtggca ttcgtaagcg
      361 tgatcaggtg ggtattcgtg tcgatcagca cgacaccctc gcgcatcgtc tcgaggatgc
      421 gcgcctgcgt gaacagcgtt tcctgctggg tcgaccgttc agtcggagca ctcatcggat
      481 cctacgcttg ccggcgggct gcgacgcccg tcacgtcgcc gtggagagac agtttccaac
      541 ggaacccggt ggggagtaaa acgctctctc ttacatgcga cataagaagg gagcgttcct
      601 acagtgccga cgaagatgaa cccgtggcgg ctgtggcagc cgccgagtga gacgatcgaa
      661 gccattgcgg gcgtgctgcg ctcgcactgg gccgcggcca agtcgctgca gcctgaggca
      721 ctctggagtg gacctgcgac ctggctgcgc cggcaggcga atcgcctcgt cgagccgacc
      781 gctccgatcg tcgagaatcc tgaaccgccg gagagcgagc tcaccatctg accagcggcg
      841 gcaggctcat gaggtaagcc tccggatccg catccgtact gaaaccgaac ttcgtgcccc
      901 ggtcgtagac caggttgaat tcgacgtagc gaccgcgttt cacgagcagt ttttccctcg
      961 cgccgtcatc gaacggcgtg tccttccgac gagccacgat ctgcgggtac acggcgagaa
     1021 acgcctcccc caccgccctg acgaacgcaa aatccgcttg ccggtctgcg ctcgagagct
     1081 catcgaagaa aattccgccg accccgcgcg gttcctgccg gtgcggtaaa tagaagtacc
     1141 gatcgcacca cgccttgaac tgcgcgtacg catccggccg gtaggtctcg caggcgccct
     1201 gcagcgccgt atgaaattgc gccgtgtcct ccgagaatgg aaacgtgggc gtgagatccg
     1261 atccgccgcc gaaccacatc cggctcgtgc gcaggtaacg cagattcaag tgcaccgccg
     1321 gcacatacgg attccacatg tgcgcgacga gtgagattcc gcacgcgacg aagcggcccc
     1381 ccgactcctc ggctccaggc acctgcgctc gcgcctgcgg cgagaactcg ccccagacat
     1441 gactgaagtt cacgccgacc ttctcgaaca cctcaccgcg cagcagacgc gactcgcctc
     1501 cgccttgcag gcgatggccg gccgggcgcg cccagctgcg cggctcgaag cgccgggtcg
     1561 attcgaatcc ctcgaagctt gcgcagatcc gactctgcag atcccgaaag tacgcggccg
     1621 ccgaatcgac caggtcagcc ggttcgctca tgcacgtggt ccacgagacg cgccacggag
     1681 tcaggatcgg tgttaggcca gatcccatgc ccgagattga agatgtggcc cggtgccgcg
     1741 cgaatctcac ccgagccgcg ggcctcaccc gaccgctcga tgtcacccga cgcctcgcgc
     1801 gctgcccggc cggcctcgcg cagcacctcg tctgcgtgcc gccgcaagtc ctcgggggac
     1861 gagaacagcg ccgccggatc gaggttgccc tgcacggcct tgtgggggcc cagcacctgg
     1921 cggaccttgc cgagcggggt ccgccaatcg acgccgatca catcaacatc caggtcggcc
     1981 acgcgctcca tcagagcgga tccctggttc aggtaataaa tgagcggaac gtccgtggag
     2041 cgacgcagag ccgcgacggt gcgacgcacc gcacgcaagg cgaagcggtc aaacgcaccg
     2101 ggccccaaca aaccgcccca cgattcaaac aacatcagag cttgggcgcc cgccgcagcc
     2161 tgcgcgtgca gataggcggc catcgcatcg gcaaggcgat cgagcagccg ctcggcggac
     2221 tgtggctgcg cgtagagaaa cgagcgcgcc acatcaaact ccttcgaggg gcgcccgctc
     2281 acgagataac acaacagcgt gaacggtccg cccgcgaagc cgatcagagg cacgccgcgc
     2341 ggcagatttg gccggatcag ctggatcgat cgcaacacga agggaacatc gcgctcgggc
     2401 acgagctcgt gcagcgcatc gatctgcgtg tccgtgcgaa tggggttgcg gacgacgggg
     2461 gcgggttcga aggtgaggtc cacgcctaga ctttgcacag gagtcatgat gtcgctgaag
     2521 aggatggcgg catccagctc gaagcggcgc aggggctgca gggtcacttc cgcggccagc
     2581 tccggctggc gggtgaggga ctcgaagtcg accttggcgc gcagctcgcg gtactccggc
     2641 aggtagcgcc ccgcctgtcg catgacccag atcggggtgt gcgccaccgc ctgccggcgg
     2701 caggcggcca gaaaagtgga ttcccgcggc gggctcattc ggccagccac ttcgccagct
     2761 gcggcgcgaa gtaggtgatg atgagatcgg ccccggcccg cttgatgccc gtgagggtct
     2821 cgagggccgc ggcccgctca tcgatccagc cgcgctcggc cgccgccttg atgaggctgt
     2881 actcccccga gacctggtag gccaccacgg gcagatgcac ggcatcgcgc acctgtcgga
     2941 tcacatcgag ataggggccg gccggcttca ccatgaccag atccgcgccc tcctccacat
     3001 cctgcaggac ctcgcgcagc gcctcgcggg cattggccgg atccatctga taggagcgcc
     3061 gatcgccaaa ggcgggggcg gattcagctg cttcccgaaa cggtccgtag aatgaggagg
     3121 cgtatttcgc cgcatatgag acgatcggcg tggtctcgaa gccttcgcga tcgaggagct
     3181 ggcgcatcgc gagaacccgg ccgtccatca tgtcgctggg cgcgacggca tcggcgccag
     3241 cccgcgcgta gttcagcccg acctgcgcca ggctctccac cgaggcatcg ttgtcgatcc
     3301 ggccattcgg cagcacgtgt ccgcagtgac cgtggtcggt cgagccgcac aggcacacgt
     3361 ccgcccagac caaaagctgc gggcacgcat ccttgatggc cgagatcgcc tgcgccgcga
     3421 gtcccttggg atcgagcgcc gcgctggcct tggcatcctt ctgcgcgggc gccgcgaaga
     3481 tcagcaccgc cggcacgccc gcggccgcca cttcgcgcgc ctccgcgacg gcttcatcga
     3541 ccgagagctg gctgatgccc ggcatgcttt ccacgcggtg acggaccccc cggcccggaa
     3601 cgacgaacag gggatagatg cacgagtcca gtgacagatg tgtttcacga accatgcgtc
     3661 gccagagatc ggactgtcgc gtgcggcgag gtctgtgcaa tggaaatccc atgatcaatg
     3721 cctcgtctgc aataaacgta acgtcgtctg ggcgagcccg gccagcgtcg ccgactcggc
     3781 gagcaccgcg gtgcagcccc gatcgaggag cgcgcgcgcc gtggtgggcc cgatcgccac
     3841 cgctctcgcc ccggcgagca gtcgctcgaa gtccttgcgc cccagcgcct gctccagttc
     3901 gatgaccgcc gaggggctgg cgaaagtcac cgccgcgatc ccgtcccgag cgatccaggt
     3961 gcggcactcc tccacgtcca gtggcgcccc gtcggtgcga taggcctcga cctgcaggac
     4021 ctccgcgccg agctgtcgga gtccggtcgc aatcgtgggc aatgcgcgcg agctggccgg
     4081 aaacaacact ttcgcaccgc ggatcggccg tgttgcaaag gccgcgacga gggccgccgc
     4141 gttggcctcg ctgggaagca actccatccg ccagccacgc tgcttcagca cctgcgcggt
     4201 cgcctgaccg accgctgcga ccaacacgcc ctgaggaggc gagctcaccc gcgcggtgac
     4261 cgcggccact gcatggcggc tggcaaacac gatccagtcg aactggccga tcgccttcag
     4321 ggccgcctcc agcggctcct tcttcgccgc cgccacactg aatgccggcc acagcaacac
     4381 cgacagcccc aaagtgcgca gctgcgtgct caggggtcca tcgttgctct ccgcacgcgt
     4441 cacgacgacc ggccgggtct cgcgcgccac accaacttcc cggttgtccg gcgtcgcgat
     4501 gttcacggct cctccacttg atttctcgct gcgcgctcgc tggcaaccag ccgcgccgcg
     4561 ccctggcgaa g
//
LOCUS       DASRHN010000142         4868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2190796, whole genome shotgun sequence.
ACCESSION   DASRHN010000142 DASRHN010000000
VERSION     DASRHN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190796"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..915)
                     /locus_tag="VFB37_05420"
     CDS             complement(19..915)
                     /locus_tag="VFB37_05420"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZO21925.1"
                     /translation="MSPRGPQLGLWIRAVRVHQWVKNLLIFVPLLASHQLGKHAVLLH
                     GLLAFALFSLCASSAYLVNDILDLPHDRQHPEKSRRPIASGQVSVRAARVISPLLLIV
                     SLGCAYWLLPHLFSAALAAYYLVSIGYSTWLKRFVIIDVVTLAMLYTLRIAAGALVFQ
                     LVLTFWMLAFSIFIFLSLAFAKRYTELNGARNRGDSRQIGGRGYYPQDLAVISSLGGA
                     SGYLSVLVLALYIQDQNTTVLYRHPQLIWLACPLLLYWISWVWMLTHRGRMYDDPVVF
                     AVRDRASLIVGVLFGLVFWLAS"
     gene            complement(912..2213)
                     /locus_tag="VFB37_05425"
     CDS             complement(912..2213)
                     /locus_tag="VFB37_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21926.1"
                     /translation="MSVASSSPLSERSFVWVGIGAIVLIRLVVILLTPRTADFLDPRI
                     YQGAGRTVLAGVNPYDYSDQPAKRAALRASMSAQVPGPDDFTQDQKSWDYYVSGNPPA
                     STALYALFESIAHGSRFAWRLLLMLGDVSLFAGMVALVKAVRGPLTRGIDQAGIACLA
                     IINPVLIVSGCAIPEDKQFQTALLLWGAALLLAPAARERRGGLGTGLVLSLSVLFKLL
                     GAFLFPLWLARAMREGPRFAIWTGLGALLPVVAAFAGFGTHFISEMLGRAVQNSVVAP
                     EHASPWVWWPALAQGHYYLPVKILVTVLFCATLAMLLARRSIDLLNFCAGLTVAFVCI
                     WLDKGAMNRMNIGIVFATAALITLSPSVFLRYCLGTVLAAGVAYVLGVGLLHAHLETV
                     DAVLALLFLVAYLLTLLGLSRTRAVDSTSSAHGALSPERPS"
     gene            complement(2210..3523)
                     /locus_tag="VFB37_05430"
     CDS             complement(2210..3523)
                     /locus_tag="VFB37_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZO21927.1"
                     /translation="MAANSARPIAATPILAAMVAGALVGWLVGPEGRLAGVPMLGFFE
                     FLGTIFINLLKMVVVPLISASIITGVASLGSGRDLGRLGIKTLAFYVFTTLLSVLIAL
                     SLVDLIRPGIVNGVPARSLLALEASSDIVAASVQQRAASTLYDTLLSIVPTNVLEAAA
                     SNKLLGVMFFSILFGFFLARIELPYRQLMLEFWQALFRIMMRMTIWVMRLAPFGIFGL
                     TARVVAKSGVHAAGPILAFGFCVVAGLAVYSVVILPLLMLLSGVRRPLRLFSATSPAL
                     LTAFSTASSAATLPLSLECLQKRAGISERVASFVMPLGTSINHAGSALYECAGAMFIA
                     QAYGLQLSFAQQFMVVTLALLTSMGVAGIPAASLVAIAVILSAVGLPPEGIGALLVID
                     RLLDMGRTAINVFADCACTAIVARLEGEKNVLEPPPAETATVPST"
     gene            complement(3543..>4868)
                     /locus_tag="VFB37_05435"
     CDS             complement(3543..>4868)
                     /locus_tag="VFB37_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZO21928.1"
                     /translation="ADKYSADIQVDDAAVQAYYKAHQAEYMSPEQVHLQYAELRLDTL
                     AAQVKPSEADLKAAYEKNKDRYVEPEKRHASHILIQVGKDDAAARKRAEDVLAQARAG
                     KDFAALAKQYSQDPGSASKGGDLGWADRNQFVKPFSDALFSMKAGEITGPVKTEFGYH
                     IIKLDEIQTGKAKSFDEVRGELEAELKRNAASDRFGEIQEQLQAKVDQSGNDLDVLAK
                     EFNLQTGDVPKFLRGAGAAPLGVAQPVQDLVFGDSPLQTGRVGGPVMVGEDRLVLVKV
                     LDHRKPEPKPIAEVRDAIVAVIRKERGSQAALKAAQEGVAKLEAGGSFDDVVKGLGVA
                     AQPARFVGRKDTTMPAQLLQVVFNTPKPEKPVYRAVALQTGGAALLAVTKLRTGPPQG
                     TPQELQAQLQQQAQLVRQNAERHGQTDAMAYVEEMRRTADVRKNPKAFE"
BASE COUNT          998 a         1591 c         1541 g          738 t
ORIGIN      
        1 aggtcagccc gctgcgcatc atgaagccag ccagaacacc agcccgaaca gcacccccac
       61 gatgaggctg gcgcgatcgc gtaccgcgaa gacgaccgga tcgtcataca tccggccgcg
      121 atgggtgagc atccagaccc agctgatcca gtagagcagc agcgggcagg cgagccagat
      181 gagctgcggg tggcgataca gcacggtcgt gttctgatcc tggatgtaaa gggccaggac
      241 cagcaccgaa aggtaccccg aggccccgcc gagcgaggag atcaccgcca ggtcctgagg
      301 atagtagccc cggccgccga tctgccggct gtcgccccga ttgcgcgctc cgttcagctc
      361 ggtgtagcgc ttcgcgaagg ccaggctcag gaagatgaaa atcgagaagg ccagcatcca
      421 gaacgtcagc accagctgga agaccagcgc gcccgctgcg attcgcaggg tgtagagcat
      481 cgcgagcgtc acgacgtcga tgatgacgaa tcgcttgagc caggtcgaat agccgatcga
      541 gacgaggtag tacgccgcaa gcgccgcact gaacaggtgg ggcagaagcc agtaggcgca
      601 ccccagcgag acgatcagga gcaacggcga gatgacccgc gctgcgcgca ccgacacctg
      661 acccgaggca atcggccttc gcgacttctc ggggtgttga cgatcgtgag gcagatccag
      721 gatgtcgttg accaggtagg cgctcgaggc gcacaggctg aacagcgcga atgcaagcag
      781 gccgtgaagc aagaccgcat gcttgccgag ctggtgactg gccagcaatg gcacgaagat
      841 cagcagattc ttcacccact ggtgcacccg gaccgcacgg atccacagac ccagctgagg
      901 ccccctcggg ctcacgaggg gcgctcggga gagagagccc cgtgagcaga ggatgtcgaa
      961 tcaacagctc gcgtgcgcga gagcccgagt agagtcagca gataggcgac gaggaacagc
     1021 agggccagca cggcgtcgac cgtttccaga tgcgcgtgca ggaggcccac gccgagcaca
     1081 taggccacgc ccgcagccag cacggtgccc aggcaatatc gcagaaacac gctcggggac
     1141 agcgtgatga gggccgccgt cgcgaacacg atgccgatgt tcatgcgatt catcgcgccc
     1201 ttgtcgagcc agatgcagac gaatgcgaca gtcaggccgg cacagaaatt caacagatcg
     1261 atgcttcgcc tcgcgagcag catcgccagc gttgcacaga agagcaccgt gaccagtatc
     1321 ttcaccggca ggtagtagtg cccctgcgcc agggccggcc accagaccca gggcgaggca
     1381 tgctcgggag ccacgacact gttctgcact gcgcgcccga gcatctcact gatgaagtga
     1441 gtcccgaacc cggcgaacgc ggccaccacg ggaagcagcg ccccgaggcc tgtccagatc
     1501 gcgaatcgag ggccttcacg catcgcccgt gcgagccaca gcggaaagag aaatgcaccg
     1561 agcagcttga acagcacgga cagcgacagc accagaccgg tgcccaatcc accgcgtcgt
     1621 tcgcgggccg ccggcgcgag cagcagtgcg gccccccaca acagcagcgc ggtctgaaac
     1681 tgcttgtctt ccgggatggc gcaccccgag acgatcagca ccgggttgat gatggccagg
     1741 cacgcgattc ccgcctgatc gatcccccgc gtgagcggac cgcgcactgc tttcaccagc
     1801 gcgaccatcc cggcaaacag gctcacgtcg cccagcatga gcagcaaccg ccatgcaaac
     1861 cgcgacccgt gagcgatcga ctcgaaaagc gcgtacagag cggtcgaggc cggagggttg
     1921 ccggaaacgt agtagtccca tgacttctga tcctgagtga agtcatccgg gccagggact
     1981 tgagccgaca tgctggcgcg cagcgcggcg cgttttgcgg gctggtccga gtagtcgtac
     2041 gggttgacac ctgcgagcac cgttcggccc gccccctgat aaatgcgcgg atcgaggaaa
     2101 tccgccgtcc gtggcgtgag caagatgacc accaggcgga tcagaacgat tgcgccgata
     2161 cccacccaca cgaagctgcg ttcggacagc gggctcgaag aggcaacact catgtcgatg
     2221 gcacagtcgc tgtttctgcc ggcggcggct ccagcacatt cttctcgcct tccaggcgcg
     2281 ccacgatcgc cgtgcatgca cagtcggcga agacgttgat cgcagtccga cccatgtcca
     2341 gcaaacggtc aatcaccagc agcgcgccga tcccctcggg cggcagcccc acggccgaca
     2401 ggatgacggc gatcgccacg agagaggccg ccggaattcc cgcaacgccc atggaggtga
     2461 gcaacgccag cgtcacgacc atgaactgct gtgcgaagga gagctgcaga ccataggcct
     2521 gggcgatgaa catcgctcca gcacattcgt agagcgcact gccggcatga ttgatcgaag
     2581 tgccgagagg catcacgaag ctcgccacac gctcggagat gccggcccgt ttctgcaggc
     2641 attccaggct caacggcaag gtcgcggccg atgaggccgt agagaacgcg gtgagcagtg
     2701 caggactcgt ggccgaaaaa agccgcagcg gccgccgcac gcccgagagc agcatcagga
     2761 gcggcaggat gaccacgctg tacacggcaa gacccgccac cacgcagaac ccgaaggcca
     2821 ggatcggccc agcagcgtgc acacccgact tcgccacgac gcgggcggtc aatccgaaaa
     2881 tcccgaaagg cgcgagcctc atgacccaga tcgtcatgcg catcatgatg cggaacaggg
     2941 cctgccagaa ctccagcatg agctgccggt aggggagctc gatgcgcgcc aggaagaaac
     3001 cgaacaggat gctgaagaac atgacaccca gcagcttgtt ggaggcggcg gcctccaaca
     3061 cgttagtggg gacgatgctc agaagcgtat cgtaaagagt cgaagcggcc cgttgctgca
     3121 cactcgcggc gacgatatcg gaggaagcct cgagagccag cagcgagcgc gccggtacgc
     3181 cattcacgat gcccgggcgg atcaggtcca ccaggctcaa cgcgatcaac accgacagca
     3241 gggtcgtgaa tacgtagaac gcgagcgtct tgatcccgag acgcccgaga tcccgtcccg
     3301 agccgaggct ggccacgccc gtgatgatgg aagcagagat caggggcacg acgaccatct
     3361 tcagaagatt gatgaagatg gtgcccagga actcgaagaa gccgagcatg ggtacgcccg
     3421 cgagacgccc ttcagggccc acgagccagc cgaccagcgc accggccacc atggcggcca
     3481 ggatgggtgt ggcggcgatc ggacgggcgg agttcgcggc catcgcgagg cggctgcgca
     3541 gcttactcga aggctttcgg gttcttgcgc acgtcagcag tgcgcctcat ttcctcgacg
     3601 taggccatcg catcggtctg tccgtgccgt tctgcgttct gcctgaccag ctgcgcctgc
     3661 tgttgcagtt gcgcctgcag ctcctgcggg gtgccctgcg gaggcccggt gcgcaacttc
     3721 gtcaccgcga gcagcgcagc gcctccggtc tgcaatgcca cggcgcgata cacgggcttc
     3781 tcgggcttcg gagtgttgaa cacgacctgg agcaattgcg ccggcatggt cgtgtccttg
     3841 cggcccacga agcgcgcagg ttgcgccgcg acgccgagcc ccttcaccac atcatcgaac
     3901 gagccgcccg cttccagctt cgccacgccc tcctgggccg ccttcaatgc agcctgagag
     3961 ccgcgctcct tgcggatgac ggcgacgatc gcatcgcgca cttcggcgat cggcttgggc
     4021 tcgggcttgc ggtggtcgag caccttcacc agaaccagcc gatcctcgcc caccatcacc
     4081 ggtccgccga cccggccggt ctgcagcgga gaatcgccga agaccaaatc ctgtaccggc
     4141 tgggccacac ccaaaggcgc tgcgccggcg ccgcgcagga acttcggcac atccccggtc
     4201 tgcaggttga attccttggc gagcacgtcc aggtcattgc cagactgatc caccttggcc
     4261 tgcaactgct cctgaatttc gccgaatcga tcggaggcgg cgttgcgttt gagctcggct
     4321 tcgagctcac cgcgcacctc atcgaagctc ttcgccttgc ccgtctgaat ctcatcgagc
     4381 ttgatgatgt ggtagccgaa ttcggtcttc accgggccgg tgatctcgcc ggccttcatg
     4441 ctgaagagcg catccgagaa aggcttcacg aactggttgc ggtcggccca gccgagatcc
     4501 ccgcctttgc tggcggagcc gggatcctgc gaatactgct tggcgagcgc ggcgaagtcc
     4561 ttgccggctc gggcctgagc caggacgtcc tccgcgcgtt tgcgagcggc ggcatcatcc
     4621 ttacccacct ggatgagtat gtggctcgcg tggcgcttct cgggctccac atagcgatcc
     4681 ttgttcttct cgtaggcagc cttcaggtcc gcctcgctcg gtttgacctg agcggccaga
     4741 gtatccaggc gcagctcggc gtactgcagg tgcacctgtt cggggctcat gtattcggcc
     4801 tggtgcgcct tgtaataggc ctgtaccgcc gcatcgtcca cctggatgtc ggcactgtac
     4861 ttgtcggc
//
LOCUS       DASRHN010000143         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2026918, whole genome shotgun sequence.
ACCESSION   DASRHN010000143 DASRHN010000000
VERSION     DASRHN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026918"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VFB37_05440"
     CDS             complement(<1..1052)
                     /locus_tag="VFB37_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZO21929.1"
                     /translation="MTSFRRRALGASLSFILTLCAGCAADRLHREGLAAVDRGDYEDG
                     VAELQQAVARQPGNMAYRLDFEARRESAVERLVAAADAARSANQLDAAAAAFKRVLRI
                     DPSNDRALHGLAGLDADRQHGAALVEARKDFDRKDYDAAEAKLQVILNEDPGYKSAQA
                     LEAAINIARGPTTVAPRLRTRDNHNVTLQLRDAPTKMVFELLQRETGINFILDKDVKS
                     DSKTTIFVQDVPVDQALDLVLDQNQLARQILASNMVMIYPNNPNKQKEYEQQMVRTFY
                     LTNAEPKDVEAMLKAVLGAKNLFIDDRAGAVILRDTPDAVRMAEKLVASVDVAEPEVM
                     LEVEVLEMSSSKLQDLG"
     gene            complement(1049..1690)
                     /locus_tag="VFB37_05445"
     CDS             complement(1049..1690)
                     /locus_tag="VFB37_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21930.1"
                     /translation="MSRFLAIPLALPRTLEFGLSRISRMKLLAIALCAAALLGATYFV
                     LRVWGPGAGTSAIHSELTPAQRASVRAARTRAMANARGRDANRISRSQQQPSIVRVRS
                     ADISHADALFASHSWYVAPPPPPPAPPSPSAASLAPPVPTAPPLPFQYIGSYKAEGEA
                     QVFFLTNGDRVYDAKIGDTLDSTYSVDSFNGSQLVLTYKPLNIKQQLSAGGSQ"
     gene            complement(1687..2274)
                     /gene="pilO"
                     /locus_tag="VFB37_05450"
     CDS             complement(1687..2274)
                     /gene="pilO"
                     /locus_tag="VFB37_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZO21931.1"
                     /translation="MTRTEPTALLLVRGWLERASQQWVPRITWSVQRTGRTGLCGIAL
                     LLASLIFLVSTHLPLTREVESLRSQVTLTHAQAATAPVSVADSGAALLHRLPGRAQMP
                     ALLGVLLQQADNAHLSMDTGKYETTTLKSGGVVSYQISFPVSGPYPRVRQFIDSTLAA
                     LPAVAVTELSLTRKAIGDGTVEAQIRLTAFTQDRP"
     gene            complement(2331..>2602)
                     /locus_tag="VFB37_05455"
     CDS             complement(2331..>2602)
                     /locus_tag="VFB37_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21932.1"
                     /translation="VPWSSLLTELESASEDVASTVSLLEVEPDPIKHVVRITAEARTL
                     SDALAYLERLQKSSMLRYPMLDSHELRKDDPQHPIRVKLSAEWHT"
BASE COUNT          459 a          829 c          840 g          474 t
ORIGIN      
        1 ccgaggtcct gcagcttgct cgaggacatt tcgagcactt cgacctcgag catcacttcc
       61 ggctcggcga catcgacgga ggcgacgagc ttttccgcca tgcgcacggc atccggcgta
      121 tcgcgcaaga tgaccgctcc cgcccggtca tcgatgaaca ggttcttggc acccagcacg
      181 gccttcaaca tcgcctcgac atccttgggt tccgcgttcg tgagatagaa cgtgcgaacc
      241 atctgctgct cgtactcctt ctgcttgttc gggttgttcg ggtagatcat caccatgttc
      301 gaggcgagga tctgccgggc gagttggttt tgatccagca ccaggtcgag cgcctggtcc
      361 acgggcacat cctggacgaa gatggtcgtc ttcgaatcgc tcttgacgtc cttgtcgaga
      421 atgaaattga ttccggtctc gcgctgcagg agctcgaaca ccatcttcgt gggcgcatcg
      481 cgtagttgca gcgtcacatt gtggttgtcg cgtgttcgca ggcgcggcgc gacggtcgtt
      541 ggcccccgag cgatattgat cgccgcttcg agggcctgcg ccgacttgta accgggatcc
      601 tcgttcagaa tcacctgcag cttcgcctcc gcggcgtcat agtccttgcg atcgaagtcc
      661 ttgcgtgcct ccacgagcgc cgctccatgc tgtcggtcgg catcgagacc cgccagaccg
      721 tggagggccc gatcgttgct cggatcgata cgcagcacgc gtttgaacgc ggccgccgcg
      781 gcatcgagtt gattcgcact gcgcgccgcg tccgctgccg cgacgagccg ttcgaccgcc
      841 gactcgcgcc gggcctcgaa atcgaggcga taggccatgt tgccgggctg acgggccacg
      901 gcttgctgca gctcggcgac cccgtcctcg tagtcgcccc ggtcgacggc cgccagaccc
      961 tcccggtgta gccggtcggc ggcacagccc gcgcagaggg tcaggatgaa cgataaagat
     1021 gcgccgagcg cacggcgccg aaaactcgtc actgggagcc ccctgccgat agttgttgtt
     1081 ttatgttgag aggcttgtag gtcagcacca gctgcgagcc gttgaaactg tcgacgctat
     1141 aggtgctatc gagggtgtcc ccgatcttcg cgtcatagac ccggtcgccg ttggtcagga
     1201 agaacacctg ggcctcaccc tcggccttgt agctgccaat gtattgaaac ggcagcgggg
     1261 gcgcggtggg cacgggcggc gccagcgatg ccgccgatgg ggacggtggc gcggggggcg
     1321 gaggtggtgg cgcgacgtac cacgagtgac ttgcgaacag agcgtccgca tgtgagatat
     1381 cggcagaacg cacgcgaact atggacggct gctgctgcga gcgcgatatc cggttcgcgt
     1441 cgcgcccccg agcgttcgcc attgcacgcg ttcgcgccgc acgcaccgaa gcccgctgtg
     1501 ccggggtgag ctcgctgtga atcgcagaag tgccggcccc gggtccccaa acccgcaata
     1561 caaaataggt ggccccaagg agggccgcgg cgcagagcgc gatcgccagc agcttcatgc
     1621 gactgatacg tgacagcccg aactccagcg tccgtgggag cgcaaggggg atggcgagga
     1681 agcggctcat gggcggtcct gcgtgaatgc agtgagacgg atctgcgcct ccacggtccc
     1741 atctccaatc gccttgcgtg tcagcgacag ctccgtcacg gcgaccgccg gcaaagccgc
     1801 gagagtgcta tcgatgaatt ggcgcacgcg cgggtatggc ccgctgactg gaaacgagat
     1861 ctggtagctc acgacgccac cgctcttcaa cgtcgtcgtt tcgtacttgc ccgtgtccat
     1921 cgagaggtgc gcgttatcag cttgctgcaa gaggactcca agcagcgccg gcatctgcgc
     1981 acgcccaggc aggcgatgca acagagcggc gcccgaatcg gccacggaga ccggagctgt
     2041 ggccgcctgc gcgtgtgtca gcgtgacctg ggagcgcaga ctttccacct cgcgagtgag
     2101 cggcagatgc gtggagacca ggaaaatcaa actcgcgagc agtagtgcga tcccgcacag
     2161 gccggtgcga cccgtccgct gcaccgacca ggtgatgcgc ggcacccact gttgtgatgc
     2221 ccgctccagc cagccacgta ccaataggag cgctgtcggc tctgtccgcg tcaccttgcc
     2281 agggctcgac gcgggcgctg gagacgccgc cttcgcaagc gtgagcgtag tcacgtgtgc
     2341 cactccgcgc tgagcttgac ccgaatcgga tgctgaggat cgtccttgcg cagctcatgg
     2401 ctgtcgagca tggggtaacg caacatcgag ctcttctgca ggcgctcgag gtacgccagc
     2461 gcatcagaca atgtacgtgc ctccgccgtg atacgtacga cgtgttttat gggatccggc
     2521 tcgacctcga gcagcgagac cgtcgaggca acatcctcac tagccgattc gagctcggtc
     2581 agcagagagc tccagggcac cc
//
LOCUS       DASRHN010000144        12440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2356440, whole genome shotgun sequence.
ACCESSION   DASRHN010000144 DASRHN010000000
VERSION     DASRHN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12440
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356440"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..2869
                     /locus_tag="VFB37_05460"
     CDS             62..2869
                     /locus_tag="VFB37_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO21933.1"
                     /translation="MRAWRVTAAVLLLAGLPLCAALAADPPPPKTLKDLESHPVKISK
                     DTQLEASSAKAMENYRRFLELQKTDPQLRAEAMRRLADLNLDAGEVERMEKEISQIDL
                     QGAEAIRLYSTLLKAYPDYARNDQVLYQLARAYETTGQPDEALSTLDRIVQRYPHSPQ
                     LDEIQFRRGELLFSAKRFAEAEKAYAVVISQGKTSHFYEQSLYKHGWSLFKQSLTEES
                     LPSFGAVLDRKLVGPAGKAVRLEDLKRADRELVEDTLRVMSITFSYGEGATSLDEYVT
                     KQGERPYSYLLYSRLGDLFVDKQRYQDAATTYRAYVARDPYSDHAPNLAMQAIEAYRK
                     AGFSDLVLEGKHEYVEHYNFSSPFWQGRDRAQYPTVVQELKTNLKDVATYFHATAQKS
                     KRVEDYQEAAKWYRDYLKSFPDDPDSAATNYLLAETLFESHQYAEAATEYERTAYGYP
                     KSDKSATAAYAALVSYQKAEEGLAGNAKTEWHKRATDAGVKFAQTFPEHPDSGGVLTR
                     AAEDIFAARDLPRAITVSESILARQPPVDVARQRIAWTIIAQSHFDQGEFDKAEPAFL
                     KARELVGNDDKMRADLTERLAATVYKQGEAKQKAGDSAGAVEDYLRVARVAPDSKVRS
                     TAQYDAAAQLVNLKQWDRAIGVLEDFRRQFPKDALSEDVTRKLAVAYAQANRPGEAAA
                     EFEHIAANPTENRAVQREALMQSADLYAKANNTPKAVSMLEKFIASNPTPVADAEEAR
                     QRLADYALKSGDVTRQEYWYREIIKADSQAGAQRTDRTHYLAAKAQLALAQPARDAFR
                     NVRLTAPLKKSLVAKRKALEDAMSAYKRAADYQVADVTTAATYEMAELYRTLAHDVMN
                     SERPHNLKGDALEEYNSLLEEQVFPFEEQAIKAHEINTIRAKDGIYDEWVQKSFRALA
                     ELKPARYGKTELTQDVDAVLN"
     gene            2853..3650
                     /locus_tag="VFB37_05465"
     CDS             2853..3650
                     /locus_tag="VFB37_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO21934.1"
                     /translation="MRCSTDIRLICPLVLLLSACASAPAHKGAPAPPAVATPQSTPAA
                     ADATAAPAAAAPPSARETADFNRALGFMRSGNSTEAELEFKQMTLAYPNLSAPYVNLG
                     ILYRKAGRLDQSEAALRNAVARNASNAIAWNELGATQRLRGEFPDAASSYEKAIAADA
                     KFAAAWRNLGVVSDLYLGDPERALTALEKYQELSGEDRPVTSWIAELRQRTGKPAVKA
                     PAAGEAANAEKAAEAPPAEPGAKPPAAGPAAPSGTPPPATTAPKSGD"
     gene            3653..4306
                     /locus_tag="VFB37_05470"
     CDS             3653..4306
                     /locus_tag="VFB37_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21935.1"
                     /translation="MVQRSAGRFLTSLVAAGLLCSGALAWSQSGTPARSQPGGQTQTG
                     SPPTAPADQPSRTPSPSPPPEELEPGVNTSATPPSQDSSATPAGAAESAARTPEQSSH
                     SETGKTTTGSRSQRASGSAKESATPRTDAGSGKAHDKIELDTTQITGNRELPKVLYIV
                     PWKRSDLGDLVGKPVNSLIDEVLTPVDRDVFRRQNRYYDALKPDAAKDAAAGSAAKP"
     gene            4456..5103
                     /locus_tag="VFB37_05475"
     CDS             4456..5103
                     /locus_tag="VFB37_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZO21936.1"
                     /translation="MDLWNVIVRFFQGGGDFMYPIAIVFVVGLSIAIERYIYLTHTAV
                     RNRTLWNEVVPMLAQGNFRQVVQVTSKSNSAIGHILNYGLARIQSARRRDDIEKAMEE
                     SLMEVVPRLERRTHYLATFANLATLLGLLGTVMGLIRAFAAVATINPAEKANLLSASI
                     SVAMNCTAFGLMTAVPLLFIHAFLQTKTTELIDSLEMASVKFLNAITERQPAQAA"
     gene            5142..5720
                     /locus_tag="VFB37_05480"
     CDS             5142..5720
                     /locus_tag="VFB37_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZO21937.1"
                     /translation="MRRSYANKKLARHSRRPAELLLVPMIDIFTVLVTFLLMTAVFSR
                     TVILQLNLPAAQTEFRDPPPGLQLEVMVRKDQLQVADRNSGPLATLPNTPAGYNYDGL
                     TDYLKRVKAKFPEKTDASILLESDTAYDTLVQVMDRVRVFEVKEGLNTVQAELFPDIS
                     IGDAPAPDTAPGGTSAAAPPPAPAAAPGAPHT"
     gene            5717..6241
                     /locus_tag="VFB37_05485"
     CDS             5717..6241
                     /locus_tag="VFB37_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZO21938.1"
                     /translation="MSMSNRARRMAQHHLRHRADAELNLIPLIDILSVMVAFLLVYST
                     EVEVIQNSKGIEIPQSIAETAPKHSVVVMITKEDLFVQGERIATVQEVRAAQDPIIAP
                     LRAALKRPLLIGKEMTERDLAQREITIMADKSLPYEVLKKVMATCTDADYGKISLATL
                     QKEKPVVAAQFRPS"
     gene            6260..7183
                     /locus_tag="VFB37_05490"
     CDS             6260..7183
                     /locus_tag="VFB37_05490"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HZO21939.1"
                     /translation="MTTLAPFYREYELPWEVDSEASARFRKILRVLLIVFVAFGILFP
                     LLPTPKRSAAETEVPQRLARVMIENKPKPPPPPPPVPKEQPKPKIEPKPVPVPPPVDR
                     KQLAHQKAQKQLNQVKDELADLREQMDLTPLSQTKNLSGAVGADSHAERSLITSKVGA
                     GSGGITSANSSRGFGTGAGSLTGHDTTAVTSSIARSGLDSRGPTRTGSSGKAARSREE
                     IELVFDHNKGAIYGLYNRALRERAELQGKLVLEFTIAPSGEVTMCRVVSSELNDPDLE
                     SKIVARVRLFHFEPKDVEPITTTKPIDFFPS"
     gene            7180..7539
                     /locus_tag="VFB37_05495"
     CDS             7180..7539
                     /locus_tag="VFB37_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21940.1"
                     /translation="MNLKSEIEYFTELRLLDKARLLNMFLHELAEEARGTYGPGAEQV
                     HDAAHLRFVNELVHRLTRVIEQQLAEDATRPADDVVLRMLLAPRADKVAERLVYNAYA
                     RAIHGFESYDTTVLMNS"
     gene            7652..8317
                     /locus_tag="VFB37_05500"
     CDS             7652..8317
                     /locus_tag="VFB37_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZO21941.1"
                     /translation="MENIQPTERTKVVRHRERGQYGRDTVNAILDEALICHVGYTLEG
                     QPYVVPTTHARIGDRLYLHGAMANRMLRTMKTGIPVCVTVTLLDGLVLARSAMNHSMN
                     YRSAFILGIAREVSEEKEKRAALDALVNHVVRGRAAEIRPPSPLELRTTIVLCLQIAE
                     ASAKVREGPPIDDEEDYALAFWAGVLPLTLVPGSPIEDPRLAAGAHVPENIAAYARPG
                     WRR"
     gene            8406..10382
                     /locus_tag="VFB37_05505"
     CDS             8406..10382
                     /locus_tag="VFB37_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZO21942.1"
                     /translation="MTEPLSQILILLATAVFAVTLVRRVALPTTVAYLAVGLALGPHA
                     LALVSDSNTARLLGELGVAFLLFTLGLEFSLPRMVAMRREVFGLGAAQVVGTTTIFAG
                     IAWLLGLPPLTGVAIGGAIAMSSTAILLQQLTERAELNRTHGRLAFSMLLFQDLAFVP
                     LLALTSALAAGGGHFDLRQGAFAVIGGALSVGIVLAAGRWLLRPLFHEIAHSRLRELF
                     TLTVLLVVLASAWISNIAGLSLALGAFLSGMMLAETEYRHQIEAAIRPFRDILLGLFF
                     ISVGMLLDVRLLIGSLGLVSTLLIGMIVLKTVVAALVTRPFTDSRFKALRTGIVISIG
                     GEFGVALCTIALQSGLIPAALGEPLLVAIVLSMVLSPFILNNNKRVARFLLKERGPPG
                     TASEREDAATGEIAQREHVILCGFGRVGQNVARVLEGHGFEYIALDLDPARIRAARQA
                     GDPVIFGDSSDEEMLGRAGLDNASAVVISFSDPATSIGILRSIRALRPEVPVLVRTQD
                     DARLQELKEAGATDVVPETFEASLMLVSHVLMLLHVPVTRVVRALGEIRSNRYSVLRN
                     IVPRGEPRPMDDTHEHREELKSIVVPPGAWAVGRTLHDVRSRGVEVTFTGVRRQGILG
                     REPAEDTVLRDGDIVVIYGQPEDLERVEAVLLAG"
     gene            10464..10850
                     /locus_tag="VFB37_05510"
     CDS             10464..10850
                     /locus_tag="VFB37_05510"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO21943.1"
                     /translation="MHKSRLAGFIIDCRTDDLDQAARFWSEALGYPLKASASAEDSGY
                     RLLGTGPQELHIEVQKVTHASRVHLDIESDDIEAEVRRLEQLGAKRIAQVRTWWVMEA
                     PTGQRFCVVKPQRADFGSAAKIWNPG"
     gene            10871..>12440
                     /locus_tag="VFB37_05515"
     CDS             10871..>12440
                     /locus_tag="VFB37_05515"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZO21944.1"
                     /translation="MSRSDIHFPPKHAALREDVHALGALVGEILREQGGTELFERVEQ
                     DRVAAILRRDGDEKASQEMAARSRGRPPALARDLVRAFSTWFQAVNLAEKVHRIRRRR
                     QYFLKDSQRPQPGGVEDAIAILKGRGLSLAEVLALLASLRIEPVFTAHPTESTRQTVL
                     RKYQRIAEHLFDRLDPTLTPNDARRLWSSIRIELTAGWQTEDHPREQLTVADEREHVL
                     FYLVEILYRVVPAFYEEIAQALEKVYGVPGDSIELPDVLHFGSWVGGDMDGNPDVHAK
                     TIRETLARQQHIIISTYFKECQTLAQRLPQSASRTNVAPELSKRIEEYSIVLPEARAI
                     TPARHDRMPYRVFLAQIGERLRHTYDGRPNGYQSVRQFRADIQLIATSLLANKGANAG
                     LFYVRRLLRRIDTFGFHLATLDIRQHASVLHQVIARGFDDSQWLQRPGEERRRILTDA
                     LAKDSGPAVELDALGKRNLAVFEAIMQGRHRYGAAAVGYFVVSGATGADDVLAALLLA
                     RWAAAYDKRTGEVAL"
BASE COUNT         2276 a         4040 c         4014 g         2110 t
ORIGIN      
        1 aggggccacc cccgcgcgag ccgccgtcgc aggatagccc gccgcccgga gcgcccagac
       61 agtgagagcc tggcgggtca cggccgctgt gctgctgctt gccggcctgc cgctgtgcgc
      121 ggcgctggcg gctgaccctc cgccgcccaa gacactgaag gatctggagt cgcatccggt
      181 caagatcagc aaggacacgc aactcgaggc cagctccgcc aaggccatgg agaattaccg
      241 gcgatttctc gagctgcaga agaccgaccc gcagttgcgc gccgaagcga tgcgacggct
      301 cgcggacctg aatctcgatg cgggtgaggt cgaacggatg gagaaggaga tcagccagat
      361 cgacctgcag ggtgccgagg cgatccgact gtactccacg ctgctgaagg cttaccccga
      421 ctacgcccgc aatgatcagg tgctgtacca gcttgcgcgc gcgtacgaga ccaccggtca
      481 gcccgatgag gccctcagca ccctcgatcg catcgtgcaa cgctatcccc acagtccgca
      541 actcgatgag atccagttcc gacgcggcga gctgctcttc tcagccaaac gctttgccga
      601 ggccgagaag gcctatgcgg tggtgatctc gcagggcaag acatcgcact tctacgagca
      661 gagcctctac aagcacggct ggtcgctctt caaacagtcc ctcaccgagg agagcctgcc
      721 gtcgttcggc gccgtgctcg atcgcaagtt ggtcgggccc gcaggcaagg ccgtgcggct
      781 cgaggacctg aagcgcgccg atcgcgagct cgtggaggat acgctccgcg tcatgagcat
      841 cactttctcg tatggcgagg gcgcgacttc gctcgatgag tacgtcacca agcagggcga
      901 gcggccctat tcgtacctgc tctactcgcg gctgggggat ctgttcgtgg acaagcagcg
      961 ctaccaggac gccgccacca cctaccgcgc gtacgtcgcc cgcgatcctt acagcgatca
     1021 cgcgccgaat cttgccatgc aggccatcga ggcctatcgc aaagcaggct tcagcgatct
     1081 ggtgctggag ggcaagcacg aatacgtcga gcactacaac ttcagctcgc cgttctggca
     1141 gggacgcgac cgcgcacagt atccgaccgt cgtgcaggag ctgaagacca acctgaagga
     1201 tgttgccacc tacttccacg ccacggcgca gaagagcaag cgggtcgagg actatcagga
     1261 agcggcgaag tggtaccgcg attacctgaa atccttcccc gacgatccgg actcggcggc
     1321 gacgaactat cttctggccg agacgctgtt cgagagccac cagtacgccg aggctgcgac
     1381 ggagtacgag cgaacggcct atggctatcc gaagagcgac aagtcggcga ctgcggccta
     1441 cgccgcattg gtctcctacc agaaggccga agaagggctg gcgggcaacg cgaaaaccga
     1501 gtggcacaag cgggcgacgg atgccggagt caagtttgca cagacctttc ccgagcatcc
     1561 cgacagcggc ggcgtgttga ctcgtgccgc cgaggacatc ttcgccgccc gggatctgcc
     1621 ccgagcgatc acggtgtccg agtcgatcct cgctcgtcag ccgccggtgg atgtggcgcg
     1681 gcagcggatt gcctggacga tcattgcgca gtcgcacttc gaccagggcg agttcgacaa
     1741 ggccgagcct gccttcctca aggcgcgcga gctggtcgga aacgatgaca agatgcgcgc
     1801 agatctcacc gagcggctgg ctgcgaccgt ctacaagcag ggcgaggcga agcagaaggc
     1861 cggcgacagt gccggcgccg tcgaggatta tctgcgagtg gctcgtgtcg ctccggactc
     1921 gaaggtgcgc tccaccgcgc aatacgatgc cgcggctcag ctggtgaatc tgaagcagtg
     1981 ggatcgcgcg attggcgtgc tggaagactt ccggcgtcag ttcccgaagg atgccctgtc
     2041 cgaagatgtc acacgcaagc tcgcagtggc ctacgcacag gcgaatcgtc ccggcgaggc
     2101 ggccgccgaa ttcgagcaca tcgcagcgaa tcccaccgag aaccgggcgg tgcagcgcga
     2161 ggcgctcatg cagtcggccg acctttacgc caaggccaac aacacgccga aagcggtctc
     2221 gatgctcgag aagttcatcg ccagcaatcc gactccggtt gcggatgccg aggaagctcg
     2281 ccagcgcttg gcggactacg cgctcaagag cggagatgtg acgcgccagg agtactggta
     2341 tcgcgagatc atcaaggcgg attctcaggc aggggcacag cgcacggacc ggacacatta
     2401 tctcgccgca aaggcgcagc tcgcactcgc gcagccggcg cgcgatgctt tccggaatgt
     2461 acggctgacg gcgccgctga agaaaagcct ggtggccaag cgcaaggcgc tcgaggatgc
     2521 gatgagcgcg tacaagcggg cggccgacta ccaggtcgct gacgtgacga ccgcggcgac
     2581 ctacgagatg gccgagctct atcgcacgct ggcgcacgat gtgatgaatt ccgagcgtcc
     2641 gcacaacctc aagggtgatg cgctcgagga gtacaactcg ctgctggagg agcaggtgtt
     2701 cccgtttgaa gagcaggcca tcaaggcgca cgagatcaac acgatacgcg ccaaggacgg
     2761 catctatgac gagtgggtgc agaagagctt tcgggccctc gcagagctga agcccgcgcg
     2821 ttacggcaag acggagctca cgcaggatgt cgatgcggtg ctcaactgac atccgcctga
     2881 tctgcccgct cgtgctgctg ctcagcgctt gcgcatcggc ccctgcccac aagggggcgc
     2941 cggcgccgcc tgcggttgcc accccgcaaa gcacgcccgc ggcggcggat gcaacggcag
     3001 cgcccgccgc agcggcgcca ccttcggcac gcgaaacggc ggatttcaat cgcgccctcg
     3061 gtttcatgcg cagcggcaac agcacggagg ctgagctcga attcaagcaa atgacgctcg
     3121 cttatccgaa tctttcggcc ccgtacgtca atctgggcat cctgtatcgt aaggcgggcc
     3181 gcctcgatca gtcggaagcg gcgctcagga acgccgtcgc gcgcaacgcg tccaatgcca
     3241 tcgcctggaa cgaacttggc gccacgcagc ggttgcgtgg cgagttcccc gatgcggcga
     3301 gctcgtatga gaaggcgatc gctgcggatg cgaagtttgc cgctgcctgg cgcaatctcg
     3361 gtgtcgtgtc ggatctgtat cttggcgatc ccgagcgagc actgacggcg ctggagaagt
     3421 accaggagct cagtggcgag gaccgcccgg tgacgagctg gatcgccgag ttgcgtcagc
     3481 gtacaggcaa gccggcggtg aaagcgccgg ccgcaggtga ggcggccaac gccgagaaag
     3541 cggcagaagc cccgccggct gagccaggtg cgaagccgcc agccgcaggt ccggccgcgc
     3601 cgtcggggac gccgcccccg gccaccaccg cgccgaaatc gggagattga tcatggtgca
     3661 acgaagtgca ggaagatttc tcacgagcct ggtcgccgcg ggtctgctct gcagtggtgc
     3721 tctcgcatgg agccagtcgg gcacgccggc gcgctcgcag cccggtgggc agacgcagac
     3781 gggcagcccg cccaccgctc cggccgatca accctcccgc accccaagcc cctcgccacc
     3841 tccagaggag ctcgagcccg gtgtgaatac ttcggcgacg ccgccctcgc aggattcaag
     3901 cgccactccc gcgggcgcag ccgaatccgc cgccagaact ccggaacaga gcagccacag
     3961 cgagacgggc aaaaccacga ctgggtcacg ttctcaacgt gcatcgggat ctgcgaagga
     4021 gtcagccacg cctcgtacgg atgcgggctc ggggaaggct cacgacaaga tcgaactgga
     4081 tacgacccag atcacgggca accgggagtt gccgaaggtg ctatacatcg tgccctggaa
     4141 gcgctcggat ctgggcgatc tggtcggcaa gccggtgaac agcctcatcg acgaggtcct
     4201 gactcccgtc gatcgtgacg tattccgccg gcaaaaccgc tattacgacg ccctgaagcc
     4261 ggatgcggcc aaggatgcgg cggcggggtc cgccgcaaag ccgtgagcag gccccctccg
     4321 gggccatccg caggggccgg ggcagggggc tttcggccag ttccgaggat tggatcgcag
     4381 caattttcgc ttatgtcatt cagtttcgct gggcttgggt cacgtgtggg cttgtactga
     4441 ccgggagtga cttcgatgga tttgtggaac gtcatcgttc gattctttca gggcggcggg
     4501 gacttcatgt acccgatcgc gattgtgttc gtggtgggtc tgtcgatagc catcgagcgt
     4561 tacatctacc tcacacatac cgcggttcgt aaccggacgc tgtggaacga ggtggtcccg
     4621 atgctggcgc agggtaactt ccgccaggtc gtgcaggtca cctcgaaatc caactctgcc
     4681 atcgggcaca tcctcaatta cgggctggcg cgcattcagt cggcgcgccg ccgggatgac
     4741 atcgagaagg caatggagga gagcctgatg gaggtcgtgc cgcgcctgga gaggcgcaca
     4801 cactacctgg cgaccttcgc caacctggcc acgctgctcg gactgctggg cacggtgatg
     4861 ggcctgatcc gcgcattcgc cgcggtcgcc accatcaatc ccgccgagaa ggccaacctg
     4921 ctctccgcca gtatctcggt ggcgatgaat tgcacggcct tcggcctgat gaccgcggtc
     4981 ccgctgctgt tcatccacgc cttcctgcag accaagacga cggagctgat cgacagcctg
     5041 gagatggcct cggtgaagtt cctcaacgcc atcaccgagc ggcagcccgc acaggcggcc
     5101 tgagtcgcct gacggtcatt ttgtccggct gaagtccgcg catgcgcagg tcgtacgcca
     5161 acaagaagct cgcgcgccac tcgcgccgtc cggcggagct tctgctcgtg ccgatgatcg
     5221 acatcttcac ggtgctcgtg accttcctgc tgatgaccgc ggtgttctcc cgcaccgtga
     5281 tcctgcagct gaacctgccg gccgcccaga ccgagttccg cgatccgccc ccggggctgc
     5341 agctcgaggt catggtccgc aaggatcagc tgcaggtcgc cgaccgcaac tcggggccgc
     5401 tcgcgacact tcccaacacg ccggccggct acaactacga cggccttacc gattacctca
     5461 aacgggtcaa agcaaagttt cccgagaaga ccgacgcctc catcctgctc gagtcggata
     5521 ccgcttatga cacgctcgtg caggtcatgg atcgcgtccg ggtcttcgaa gtcaaggagg
     5581 gtctcaacac cgtgcaggcc gagctgttcc cggacatttc cattggcgat gcgcctgcgc
     5641 cggatactgc tccgggcggc acctctgctg ccgccccgcc gcctgcgccc gccgcggctc
     5701 ctggagctcc gcacacatga gcatgtccaa ccgcgcgcgg cgcatggccc agcatcatct
     5761 gcggcaccgc gccgatgcgg aactgaacct gattccgctg atcgacatcc tttcggtgat
     5821 ggtcgccttc ctgctggtgt actcgaccga ggtcgaggtc atccagaaca gcaaggggat
     5881 cgagatccct cagtccatcg cggaaaccgc gcccaagcat tccgtcgtgg tgatgatcac
     5941 caaggaggat ctgttcgtgc agggcgagcg catcgcgacg gttcaggaag tgcgcgcggc
     6001 gcaggatccc atcattgcgc cgttgcgagc cgccctgaaa cggccgctgt tgatcggcaa
     6061 ggagatgacc gagcgcgatc tggcgcaacg ggagatcacc atcatggccg ataaatcgct
     6121 cccctacgag gtgctgaaga aagtcatggc gacctgcacc gacgccgact acggaaaaat
     6181 ttccctcgcc accctgcaga aagagaagcc cgtggtggcg gctcagttca ggccgtcgta
     6241 gcgggcagca cgagctttca tgactacgct ggcgcccttc taccgtgagt acgagctgcc
     6301 ctgggaagtc gattccgagg cgagcgcgcg ctttcgcaag atcctgcgcg tgctgctcat
     6361 cgtctttgtg gccttcggca tcctgttccc gctgctgccg accccgaagc gcagcgcagc
     6421 cgagaccgaa gtaccgcagc ggctggcccg ggtgatgatc gagaacaagc cgaagccgcc
     6481 gccaccacca ccgccggtgc cgaaggaaca accgaagccg aagatcgagc cgaagcccgt
     6541 cccggtgccg cctcctgtgg atcggaagca gcttgcgcac cagaaagcgc agaagcagct
     6601 caaccaggtg aaggacgagc tggccgacct gcgcgaacag atggacctga ctccgctctc
     6661 ccagacgaag aatctgtcgg gcgccgtcgg cgcggattcc cacgcggagc gctcgctcat
     6721 cacctccaag gtcggggccg ggagcggcgg catcacctcg gcgaacagca gccggggttt
     6781 cggaaccgga gcgggctccc tgaccgggca cgacacaact gccgtcacct catcgatcgc
     6841 ccgttcgggg ctcgacagcc gcggaccgac gcgcaccggc agcagcggca aggcggcgcg
     6901 ctcacgcgag gagatcgagc tggtgttcga tcacaacaag ggcgcgattt acggtctgta
     6961 taaccgggca ctgcgcgaac gggccgagct gcagggcaag ctcgtgctgg agttcaccat
     7021 cgcgccttcg ggcgaggtca cgatgtgccg cgtggtctcc agcgagctca acgatccgga
     7081 tctcgagagc aagatcgtgg cgcgggtgcg tctgtttcac ttcgagccca aggatgtcga
     7141 gcccatcacc acaaccaagc cgatcgattt cttcccctcg tgaacctgaa atcggaaatc
     7201 gagtacttca cggagttgcg gctgcttgac aaggcgcgac tgctgaacat gttcctgcat
     7261 gagcttgcgg aggaggcgcg tgggacctac ggtcccggtg ccgagcaggt tcacgatgcg
     7321 gcccatctgc gcttcgtcaa cgagctggtc caccggctca cgcgtgtgat cgagcagcag
     7381 ctcgccgagg atgcgacccg gccagccgat gatgtggtgc tgcgcatgct gctcgccccg
     7441 cgagcggaca aggtggccga acgcctcgtc tacaatgcct atgcgcgggc tatccacggt
     7501 ttcgagagtt acgacaccac ggtgctgatg aattcctgac ctgcgcccgc ggtggccggc
     7561 ccggattacc ccaactggcc cttttcgccc ggcctgtgca ggcgcatcgt aggcaacggg
     7621 cctttgcccg gcattcgcag gcgacagatt cgtggaaaac atccagccga ccgagcgtac
     7681 gaaggtagtc cggcaccgcg agcgcggaca atacggacgc gacaccgtca acgcgatcct
     7741 cgatgaggcg ctcatctgtc atgtgggtta cacgctggag gggcagccct acgtcgtacc
     7801 caccacgcac gcccgcatcg gcgacaggct ctacctgcac ggggcgatgg cgaaccgcat
     7861 gttgagaacg atgaaaaccg gcatcccggt atgcgtgacc gtgaccctgc tggatgggct
     7921 ggtgctggcc cgctcggcga tgaatcactc gatgaattac cggtcggcgt tcatcctggg
     7981 catcgctcgc gaggtcagcg aggagaagga gaaacgcgct gcgctggatg cactggtcaa
     8041 tcacgtagtc cgcggccgtg ccgctgaaat acgcccccca agccccctgg agctcaggac
     8101 taccatcgtc ctgtgtctgc agatcgcgga ggcgtccgcc aaggtacgtg aagggccgcc
     8161 catcgacgac gaagaggact atgcgctcgc cttctgggca ggtgtcctgc cactgaccct
     8221 cgtaccggga tcgccgatag aggatccgag gctcgccgct ggcgcgcatg tccctgaaaa
     8281 catcgcggct tacgcgcgac ccgggtggcg cagatgatct gatcgttgcg gactctccgg
     8341 ccgcagtcta agttcgtgcg gccgggccat gtggctcact ggacggcagc aggagacacc
     8401 tggcgatgac cgagccgctg tcacaaatcc tcattctcct ggcgaccgcg gttttcgccg
     8461 tgacgctggt gcggcgcgtg gccctgccga caacggtcgc ctacctggcc gtcgggcttg
     8521 cgctcggccc gcacgctctc gccctcgtgt cggactcgaa tacggcgcgt ctgctcggtg
     8581 agctgggcgt ggccttcctg ctcttcactc tgggcctgga gttctcgctg ccgcggatgg
     8641 tcgccatgcg ccgcgaggtc ttcgggctcg gtgcagccca ggtcgtcggc accacgacga
     8701 tctttgccgg catcgcctgg ctgctcggtc tgcccccgct gacgggggtg gccatcggcg
     8761 gggcgattgc catgagctcc accgccatcc tgctgcagca gctcaccgaa cgcgccgagc
     8821 tcaatcgcac tcacgggcgg ctcgccttca gcatgctgct cttccaggat ctggccttcg
     8881 tgcccctgct ggcactgacc tcggcgctgg ctgcaggagg aggtcacttc gatctgcgtc
     8941 agggcgcctt cgcggtcatt ggcggcgcgc tctcggtggg catcgtgctg gcggcggggc
     9001 gctggctgct gcgaccgctg tttcacgaga tcgcgcacag ccgcctgcgc gagctcttca
     9061 cgctcaccgt gctgctggtg gtgctcgcct cggcctggat ctccaacatt gccggcctgt
     9121 cgctggcatt gggcgcattt ctttccggaa tgatgcttgc cgagaccgag taccgacacc
     9181 agatcgaagc ggccatccga cccttccgcg acattctgct cggattgttc ttcatctcgg
     9241 tgggcatgct cctggacgta cgcctgctga tcggcagcct ggggctggtc tcgacgctgt
     9301 tgatcggcat gatcgtactg aagaccgttg tcgccgcgct cgtcacccgc cccttcaccg
     9361 actcccggtt caaggccctg cgcacgggca tcgtcatttc gatcggcgga gagttcgggg
     9421 tcgccctctg caccatcgcg ctgcagtccg ggctgattcc cgctgcgctc ggcgagccct
     9481 tgctcgtggc catcgtgctc tccatggtgc tcagtccctt catcctcaac aacaacaagc
     9541 gcgtagcgcg ctttcttctc aaggagcgcg ggccgccggg aaccgcgagt gagcgggagg
     9601 acgctgccac cggtgagatc gcacagcgcg agcacgtgat cctctgcggt tttggccgcg
     9661 tcggccagaa cgtggcccgc gtcctggaag gacatggttt cgagtacatc gcgctggatc
     9721 tggatcccgc ccgcatcagg gcagcgcgcc aggcgggtga cccggtgatc tttggagact
     9781 cctccgatga ggagatgctt ggcagagcgg ggctcgacaa tgccagcgcc gtggtcatca
     9841 gcttttccga ccccgcaacc tccatcggaa tcctgcgcag catccgcgcc ttgcggcctg
     9901 aggtgccggt gctggtgcgc acgcaggatg acgcccgcct tcaggagctg aaggaggcgg
     9961 gcgcgacgga tgtcgtgccc gaaacgttcg aggccagtct catgctggtg tcgcacgtgt
    10021 tgatgctgtt gcacgttccg gtgacccggg tggtgcgcgc gctgggtgag atccgcagca
    10081 accgctattc agtgctgcgc aacatcgtcc cgcgaggcga gccccggccg atggacgata
    10141 cgcatgagca ccgggaagag ctcaaatcga tcgtggtgcc ccctggagcc tgggcagtag
    10201 gccgtacgct gcacgacgtc cgcagccggg gcgtggaagt gacattcacc ggagtgcgca
    10261 gacagggcat tctcgggcgc gagcctgccg aggacaccgt gctgcgggac ggcgatattg
    10321 tcgtgatcta tggacagccc gaagacctgg agagggttga ggccgtgctg ctggccggtt
    10381 gaagggccac cttcagctag aatgaggcca aaaggacgtg agacctctca ctcgccggca
    10441 cacctttcgc tcgaggtagt ggtatgcaca aaagccgact cgcgggattc atcatcgatt
    10501 gccgcaccga tgatctcgac caggccgccc ggttctggag cgaggctctc ggctatcctc
    10561 tgaaagcgtc cgccagcgct gaggacagcg gctatcgtct gctcgggacg ggcccgcagg
    10621 agctgcacat cgaggtgcag aaggtgacgc acgcgagccg ggtacacctg gacatcgagt
    10681 ctgatgacat cgaggccgag gtccggcgcc tggagcagct cggtgccaag cgcattgcgc
    10741 aggtccgcac atggtgggtc atggaggcgc cgaccggcca gcgattctgc gtcgtgaagc
    10801 cgcagcgcgc cgacttcggt tccgctgcca agatctggaa tcccggatga acgctcctgg
    10861 cgtccggcgc atgagccgca gcgacattca cttcccaccc aagcacgcgg ccctgcgcga
    10921 agacgttcac gcgctgggcg ctctcgtcgg ggagattctg cgcgagcagg gtggaaccga
    10981 gctgttcgag cgggtcgagc aggatcgggt cgcggcgatt ctccgccgtg acggcgatga
    11041 gaaagcctcg caggagatgg cggcaagaag ccgcggccgt cccccggcgc tggcacgcga
    11101 cctggtgcgc gcattttcga cctggtttca ggcggtgaat ctggcggaga aggtgcatcg
    11161 catccgccgg cgtcgccagt actttctcaa ggacagccaa cgcccgcagc caggcggcgt
    11221 cgaggatgca atcgccatcc tcaagggacg cggcctgtca ctcgccgaag tgctggcttt
    11281 gctcgcctcg ctgcggatcg agccggtctt caccgcccat ccgactgaat cgacacgaca
    11341 gacggtcctg cgcaagtacc agcgcatcgc cgagcatctg ttcgaccggc tggatccaac
    11401 tctgactccc aacgatgcgc ggcgtctgtg gagcagcatt cgaatcgagc tgactgccgg
    11461 gtggcagacc gaagaccatc cgcgcgagca gctcacggtg gccgacgagc gcgagcacgt
    11521 gctgttctat ctggtggaga ttctctaccg cgtggtgccc gcgttctacg aggagattgc
    11581 tcaggcgctc gagaaagtct atggcgttcc gggcgactcg atcgagctgc ccgacgtgct
    11641 gcatttcggc tcatgggtcg gcggggacat ggatggcaac ccggacgtac acgccaaaac
    11701 gattcgggag accctcgcgc ggcagcaaca catcatcatc agcacctatt tcaaggagtg
    11761 ccagaccttg gcgcagcggc tgccacagag cgccagtcgc accaacgtcg caccggagct
    11821 gtcgaagcgc atcgaagagt actccatcgt gcttccggaa gcgcgcgcta tcacgccggc
    11881 ccgtcacgat cggatgccat accgcgtctt cctggcacag atcggcgaac ggctgcgcca
    11941 tacgtacgac ggccgaccga atggctacca gtccgtccgc cagttccggg ccgatatcca
    12001 actcattgca acgagtctgc tggccaacaa gggtgcgaat gcgggtttgt tttacgtgcg
    12061 gcgtctgctg cggcgaatcg acacgttcgg atttcacctg gccacgctcg acatccgcca
    12121 gcacgcctcc gtgttgcacc aggtcattgc gcgtgggttc gatgactctc aatggctgca
    12181 gcgaccaggc gaggagcgcc gccggatcct gacggacgct ctggcgaaag acagcggacc
    12241 ggccgtcgag ctggatgccc tggggaagcg taatctcgcg gtcttcgaag caatcatgca
    12301 gggacgtcat cgctacggtg ccgcggcggt tggttacttc gtggtgagcg gtgccaccgg
    12361 ggcggatgac gtgctcgcag ccttgctgct cgcgcgctgg gccgctgcat atgacaagcg
    12421 taccggcgaa gtggcgctcg
//
LOCUS       DASRHN010000145         7043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2027346, whole genome shotgun sequence.
ACCESSION   DASRHN010000145 DASRHN010000000
VERSION     DASRHN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027346"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFB37_05520"
     CDS             complement(<1..266)
                     /locus_tag="VFB37_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21945.1"
                     /translation="MASFDLPRPLSDYFAFAETTPTREGRRFSITLPYLRADGRIDRL
                     QWWYHVAEGKQKILGEEAVRAVRAKAIERFSRHIERWLCNTAQT"
     gene            complement(330..1361)
                     /locus_tag="VFB37_05525"
     CDS             complement(330..1361)
                     /locus_tag="VFB37_05525"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZO21946.1"
                     /translation="MSVRNGFAGAVGNTPLIRLRGVSEETGCNILGKAEFMNPGGSVK
                     DRAARAIIMEAERRGELAPQGTVVEGTAGNTGIGLAHVCNARGYRCVIVMPDNQSPEK
                     YRLLEMLGAEVHKVPAVSYSNPNQYQKVAQRLAATLPNAIWSNQFDNTANRAAHREST
                     GPEIWSQTEGHIDAFVAATGTGGTFAGVSEYLKSQRRAVRCVLADPPGSSLYEYVRNG
                     TLRATGSGSITEGIGIGRITANLEGAPIDDAIHVEDADTVRFVYRLLNEEGLFLGSTS
                     GINVAAAVRVALELGRGHTVVTVLCDGGAKYQSRLFNREWLQQKGLAAQAGAPPERPP
                     VSLDKVLLS"
     gene            complement(1358..2803)
                     /gene="cysG"
                     /locus_tag="VFB37_05530"
     CDS             complement(1358..2803)
                     /gene="cysG"
                     /locus_tag="VFB37_05530"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HZO21947.1"
                     /translation="MSGASAGMPYLPVSLRLQSEPVLVAGGGRVAARKVDLLRRSGAR
                     VTIVAPQLDGELQKLVASGELQHIAAPFQEPHLAGAVLVVAATHCDEVNAAIAAAARR
                     QRIPVNVVDDAAASTFIFPAIIDRSPLIVAVSSNGEAPVLARRIREQFEALLPARLGA
                     LARFMGARRRSVQRALSFAARRAFWERVAGGTVGTRVLAGDETGAARAFARELRTSHL
                     TSSPATGESGLGECYLIGAGPGDPDLLTVRALQLLQQADVILYDRLVSPGILERARRD
                     AQRLYVGKHAGSHTSQEHINELLVRYARQGLRVARLKGGDPYIFGRGGEEADVLARAG
                     IPFLVVPGITAALGAAASAGIPLTHRRLAQSVTFVTGHSVDQDTLDWCSLAGAHRTTV
                     FYMGMGELPRIIEKLRAAGASRTLPAAVIERATLPDQRVVHGTLDTIAGLARKAAISP
                     PALLIIGEVTAVLHADELAGSEDSLVDGALA"
     gene            complement(2800..3525)
                     /locus_tag="VFB37_05535"
     CDS             complement(2800..3525)
                     /locus_tag="VFB37_05535"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZO21948.1"
                     /translation="MRSRLSSDLEQRAAATRNALLDALRDHGRVVYSNSLGAEAMVLT
                     DIIWTHVPDIDIFTIDTGRLHEETYELLEKLQRRYQRTVRVVYPEARALEALVASQGV
                     NGFYHSLEARLACCRIRKVEPFTRAIAGYRAWVTGLRRTQSLARAEARSLEWDATHRL
                     YKVSPLLEWSEEQVWEYIRVHKLPYNRLHDRQFPSIGCSPCTRAIQPGESRRAGRWWW
                     EQPESRECGLHPRVPPTAQAAQA"
     gene            3687..4658
                     /gene="cysB"
                     /locus_tag="VFB37_05540"
     CDS             3687..4658
                     /gene="cysB"
                     /locus_tag="VFB37_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HZO21949.1"
                     /translation="MKLHQLRYLAAVAQSGLNITAAAQKLHTSQPGVSKQIKLLEDEL
                     GFQIFVREGRNLTRITPAGQQVIDRALRILQEAQSIRDLSTELRDEGRGSLSIGTTHT
                     QARYVLPDVIRQFRDRYPNVRLNLHQGTSEQIAEMVAQDRIDCAIATGSEQLFSDLTL
                     LPCYRWFRTVIVPREHPLASAGRLTLKGLGAYPIVTYTFSFTGPSSLHEAFARSGLTP
                     NVAITARDADVIKTYVRLGLGVGIVAHMAIDGEDADLKAIDASHVFAPHVTWIGFRRG
                     TLLRKYMYEFAQLLAPHLDRRTVDRAHRASSAEEVREMFSQTELPER"
     gene            complement(4667..5176)
                     /locus_tag="VFB37_05545"
     CDS             complement(4667..5176)
                     /locus_tag="VFB37_05545"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZO21950.1"
                     /translation="MRSILRRRELLVDDWKYSREVGAPNLANPLEAALIVTLLELRAD
                     PDLWHGRPGRLGVRLSPVDNVEELTPHLRRLELVAVEFPGPGDGRGFSQARLLRTRLG
                     FRGELRAIGAGVRQDLIFLMVRCGFDSFELAPGQTVDSALRAYERYTVAYQPGAALES
                     ITRQRFGAD"
     gene            complement(5151..6806)
                     /locus_tag="VFB37_05550"
     CDS             complement(5151..6806)
                     /locus_tag="VFB37_05550"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZO21951.1"
                     /translation="MYRYDELDQAFVDQRVAEFRDQTRRFLAGELPEEEFRGLRLRNG
                     LYIQRHAPMLRIAVPYGLLGTHQLRKLAEITERFDRGYGHFTTRQNLQLNWPRLEQVP
                     DILQELATVQMHSIQTSGNCVRNVTADHLAGVAPDEVDDPRPYCEIVRQWATLNPEFT
                     YLPRKFKIAITGSPADRAASEVHDIGLHLRRDSHGQIGFQVLVGGGLGRTPIIGQVIR
                     EFLPLPELLSYLEAILRIYNRHGRRDNIHKARIKILVRSLGVERFREQVEAEWLASRE
                     SATPLSAEQVDRVRGHFKPPAYESLADSDVSAAREPEFQAWYRYNTRAHRVPGYRAAF
                     VSLKAHGRNPGDITSAQMQAVAALADRLSFGLVRVTHAQNLLLADVRQSDLYALWGEL
                     RGLGLAMPNIGTLTDMIACPGLDFCSLANAGTLGVARRIQEQFDDLDYLYDIGEIELK
                     MSGCMNGCGHHHVGHIGILGVDKHGEEWYQITLGGSVNGFTALGEVIGPSVPQEEVAP
                     TIERLVEVYRARREDGERFIDTFRRIGPQPFKERAYAIHSAAA"
BASE COUNT         1256 a         2308 c         2284 g         1195 t
ORIGIN      
        1 aaagtctgcg ccgtattaca caaccagcgc tcgatatgcc ggctgaaccg ctcgatcgct
       61 ttcgcgcgca cggctcggac cgcttcctca ccgaggatct tctgcttccc ctcggccacg
      121 tgataccacc actgcaagcg atcgatgcgc ccgtcagcgc gtaagtacgg cagcgtgatg
      181 gagaaccggc gtccttcgcg cgtcggagtc gtctcggcga atgcgaagta atccgagagc
      241 gggcgcggca ggtcgaagct tgccatcgtt tttgtaccgg ggaccgggat cgcacggatg
      301 ataccgagtt tcggtatccg gcgggagatt caggagagga gcaccttgtc cagcgagacg
      361 gggggccgct caggaggcgc ccccgcttgc gcagccagcc ctttctgttg cagccattcg
      421 cgattgaaaa gccgcgactg gtacttggca cccccatcac agagcaccgt gaccaccgtg
      481 tggccccggc ccagctcgag cgccacgcgc accgcggcgg cgacattgat gccactggtg
      541 ctgccgagaa acagcccttc ttcgttcagc agtcgataca cgaaacgcac ggtgtcggcg
      601 tcctcgacat ggatcgcatc gtcgatcggg gcgccctcga gatttgcggt gatacgcccg
      661 atcccgatcc cctcggtgat cgagcccgat ccggtggccc gaagcgtgcc attacggacg
      721 tattcataga gactgctgcc cggtggatcc gccagcacac agcgcacggc gcgccgctgc
      781 gatttcaagt attcggacac tcccgcgaag gtgcctcccg tacccgttgc ggcgacgaag
      841 gcgtcgatgt gaccttcggt ctgagaccag atctccggtc ccgtgctctc gcggtgcgcc
      901 gcgcgattgg cggtgttgtc gaactgattc gaccagatgg cattcggcag cgtagcagcc
      961 aggcgttgcg cgaccttctg gtactggttg ggattactgt aggaaacggc cggcaccttg
     1021 tgaacctcgg cgcctagcat ctccaggagc cggtacttct cgggcgactg attgtcgggc
     1081 atgacgatca cgcagcgata gccgcgggcg ttgcacacgt gcgcaaggcc gatgccggtg
     1141 ttccccgcgg tgccctccac aaccgtaccc tgtggcgcaa gctcaccgcg ccgctcggct
     1201 tccatgatga tcgcgcgtgc cgcccggtcc ttgaccgagc cgcccggatt catgaattcc
     1261 gccttgccca gaatgttgca gcccgtttcc tcgctgacac cccgcaatcg gatcaggggt
     1321 gtgttgccta cggcgcccgc gaatccattg cgtacgctca tgccagcgcc ccatcgacca
     1381 gggagtcttc cgagcccgcg agctcgtccg catgcaacac cgccgtcact tcgccgatga
     1441 tcaacagcgc cggcggcgag atggccgcct tccgagcgag gcccgcaatg gtgtcgagcg
     1501 taccgtgcac gacacgctgg tccggcagcg tagcgcgttc gatgacggcc gccggcagtg
     1561 tgcgtgaggc gcccgctgcg cgcagcttct cgatgatccg gggtaactcg cccattccca
     1621 tataaaacac cgtcgttcga tgagctcctg cgagggagca ccagtcgagc gtgtcctggt
     1681 ccacactgtg cccggtcacg aaggttacac tctgagccaa cctgcgatgc gtcagcggga
     1741 tgccggccga ggctgcggcc ccgagcgcag cggtgattcc gggcacgacg aggaagggga
     1801 tcccggcgcg cgcaagcacg tcggcctcct cgccgccgcg cccgaaaatg tacgggtccc
     1861 cgcctttcag ccgcgccaca cgcaagccct ggcgtgcata gcgcaccagc agctcattga
     1921 tgtgctcctg cgaggtatgc gagccggcgt gtttgccgac gtatagacgc tgtgcatccc
     1981 ggcgggcccg ctccaggatg cctggcgaaa ccaggcgatc gtagaggatg acatccgcct
     2041 gttgcaggag ctgcagagcg cgcactgtca gcagatcggg atcgccggga ccggccccga
     2101 tgaggtaaca ctcgccgagt ccggattcgc ccgtcgcggg cgagcttgtc agatgagacg
     2161 tgcgcaactc tcgggcgaag gcgcgcgcgg cgccggtctc atcaccggcg agtacacgag
     2221 tgcctacggt gccgccggcc acgcgctccc agaacgcgcg ccgggcggcg aaacttagcg
     2281 cccgctgaac ggacctgcgc ctcgcgccca tgaagcgcgc cagggcaccc agccgcgccg
     2341 gcagcagagc ttcgaattgc tcacggatgc ggcgcgcgag gacgggcgcc tcaccgttcg
     2401 agctcaccgc gacgatgaga ggtgaccggt cgatgatggc cggaaaaatg aaggtcgagg
     2461 cggccgcatc atcgacgaca ttcaccggaa ttcgctgtcg tcgggccgcc gcggcaatgg
     2521 cagcattgac ctcatcgcag tgcgtcgccg ccacgaccag cacggccccg gcgagatgcg
     2581 gctcctggaa gggcgccgcg atatgctgca gctcgccgct cgccacgagc ttctgcagtt
     2641 caccatccag ctgcggtgcg acgatcgtga cccgcgcacc gctgcgacgt aaaaggtcga
     2701 ccttgcgggc ggccaccctg ccgccacccg ccacgagaac cggctcgctc tgcagccgca
     2761 gggaaaccgg caggtaaggc ataccggcgg atgcgccgct caagcctgcg ccgcctgcgc
     2821 tgtcggagga actctgggat gcaagccgca ttcacgggac tcgggttgtt cccaccacca
     2881 gcggcccgca cgacggctct cgccgggttg gatcgcgcgc gtgcaaggcg agcagccgat
     2941 actgggaaac tggcgatcgt gcagccgatt gtacggcagc ttgtgcacgc ggatgtactc
     3001 ccagacctgc tcctcgctcc actccagaag cgggctgacc ttgtacagcc gatgggtggc
     3061 atcccactcc agcgatcgcg cttcggcccg ggcgagtgac tgcgtgcgcc gcaggccggt
     3121 cacccaggca cgatagcccg caatcgctcg cgtgaagggc tcgaccttgc ggatccgaca
     3181 gcacgcgagc cgcgcttcga gactgtgata gaaaccgttc acgccctgcg aggcgacgag
     3241 ggcttcgaga gcgcgcgcct cgggatagac gacccgcacc gtgcgctggt atcgccgctg
     3301 cagcttctcg agcagctcgt aggtctcctc gtgcaaccgc cccgtgtcga tggtgaagat
     3361 gtcgatgtcg ggaacgtgcg tccagatgat gtccgtcagc accatcgcct cggcgcccag
     3421 gctgttcgaa tacacgacgc gcccgtggtc gcggagcgca tccagaagag cattgcgcgt
     3481 cgcggcggcc cgctgctcga ggtcggacga aagccttgat cgcatggtgg ggcaactata
     3541 gcgagccgcg ccgccccggc agaacgaccg attgcttctg agggactggg cagcggttat
     3601 gaacaggtga cacgccggtc atggggcggt tgcacacgcg gggtgagatt cgccgccacg
     3661 tgcacgattg cgctagtctt ccacccatga aactgcacca gctgcgttac ctcgccgcag
     3721 tcgctcaaag cggcctcaac atcaccgcgg ccgcgcagaa actgcatacg tcccagcctg
     3781 gcgtgagcaa gcagatcaag ctgctcgagg acgagctggg gtttcagatc ttcgtgcgcg
     3841 aagggcgcaa tctcacccgc atcactccgg caggtcagca ggtcatcgac cgggcgctgc
     3901 ggatactgca ggaagcccaa agtattcgcg atctctcgac cgagttgcgc gatgagggca
     3961 ggggcagcct gtccattggc acgacgcaca cgcaggcgcg ctacgtcctg cctgatgtga
     4021 tccgacaatt ccgcgaccgc tatcccaacg tgcgcctcaa tctgcaccag ggcacgtccg
     4081 agcagatcgc cgagatggtg gcccaggacc ggatcgactg tgccattgcc accggctcgg
     4141 agcagctctt cagcgatctg acgctgctgc cgtgctatcg ctggttccgt accgtcatcg
     4201 tgccgcgcga gcatccgctc gcaagcgccg gccgactgac cctgaaaggc ctcggggctt
     4261 atccgatcgt cacctatacg ttcagcttca cgggcccgtc ctcactgcac gaagcgttcg
     4321 cccgctcagg tctcacgccc aatgttgcga tcactgcccg ggatgcggat gtgatcaaga
     4381 cctacgtgcg actcggcctg ggagtgggca tcgttgccca tatggcgatc gatggtgagg
     4441 atgccgattt gaaggcgatt gatgcctcac acgtgttcgc cccacatgtc acgtggatcg
     4501 gattcaggcg cggcacgctg ctgaggaagt acatgtacga gttcgcccag cttctggcgc
     4561 cgcatctgga ccgccgcacg gtggaccggg ctcatcgggc ctcctcggcc gaggaggttc
     4621 gcgagatgtt ctcacagacc gagcttccgg agcgctgact ggcgagtcag tccgctccga
     4681 agcgctgccg ggtaatcgac tccaatgcag cacctggctg ataggcgacg gtgtagcgct
     4741 cataggcccg cagtgcgctg tccaccgtct ggccaggcgc cagctcgaac gaatcgaaac
     4801 cacagcgcac catcaaaaag atcaggtcct gtcgcactcc tgcgccgatg gcacgcagct
     4861 cgccgcgaaa gccgagccgc gtacgcagaa gcctggcttg cgagaatccg cggccatcgc
     4921 ccgggcccgg aaattctacc gcgaccagct cgagccgacg gaggtgcggg gtgagctcct
     4981 cgacgttgtc taccggggag agccgtactc cgagccgccc aggacggccg tgccagagat
     5041 cggggtctgc gcgcagctcg aggagcgtga cgatcagcgc ggcctcgagg gggttggcaa
     5101 ggttgggtgc accaacctcg cgcgagtatt tccagtcatc gacgagcagc tcacgccgcc
     5161 gcagaatgga tcgcataggc gcgctccttg aagggctgcg gcccgatgcg ccggaaagtg
     5221 tcgatgaagc gctcgccatc ttcgcggcgg gcacgataga cctccacgag ccgctcgatg
     5281 gtcggggcca cttcctcctg cggcacggac ggaccaatta cttcgccgag cgcggtgaaa
     5341 ccgttgaccg atccgccgag ggtgatctga taccactcct cgccatgctt atcgacgccc
     5401 aggatgccga tgtggccgac gtggtgatga ccacagccat tcatgcaacc tgacatcttg
     5461 agctcgatct ccccgatgtc gtagagataa tctagatcgt cgaactgctc ctggatgcgc
     5521 cgcgccacac cgagcgtgcc ggcgttcgcc aggctgcaga agtccaggcc cgggcaggcg
     5581 atcatatcgg tgagcgtgcc gatgttgggc atcgcgagac cgagcccgcg cagctcgccc
     5641 cacaacgcat acagatcgga ttggcggaca tcggccagca gcaggttctg ggcgtgagtc
     5701 acccggacca ggccgaaact gagacggtcg gcgagggcgg cgacggcttg catctgcgcg
     5761 gatgtgatgt cgcccggatt gcgcccgtgc gctttcagcg agacgaacgc ggcccgatag
     5821 ccgggcacgc gatgcgcgcg ggtgttgtat cggtaccagg cctgaaactc gggctcgcga
     5881 gcagcgctca catccgaatc ggcaagactt tcataggcag gcggcttgaa atggccccgc
     5941 acgcgatcca cctgctcagc gctgagcggt gtcgcgctct cgcggcttgc gagccactcg
     6001 gcttcgacct gctcgcgaaa tcgctccacg ccgagtgaac ggaccaggat cttgatgcgc
     6061 gccttgtgga tgttgtcacg acggccatga cggttgtaga tgcgcaggat ggcttccagg
     6121 taggacagca gctcgggcag cggcagaaac tcccggatga cctggccgat gatcggcgtg
     6181 cgcccgaggc cgcccccgac cagcacctga aagccgatct ggccgtgact gtcacggcgc
     6241 aggtgcaggc cgatgtcgtg cacctcggag gccgcgcgat cggccggaga gccggtgatg
     6301 gcgatcttga acttgcgcgg cagataggtg aattcggggt tcaaggtcgc ccactggcgc
     6361 acgatctcgc agtacgggcg tggatcgtcg acctcgtcgg gggcaacgcc cgcgaggtga
     6421 tcggcggtca cgttccggac acagttgccg ctcgtctgga tgctatgcat ctgcaccgtg
     6481 gcgagctctt gcaggatatc cggcacctgc tccaggcgcg gccagttgag ctgcaggttc
     6541 tggcgggtcg tgaagtgtcc gtagccgcga tcgaaacgct cagtgatctc ggcgagctta
     6601 cgcagctggt gcgtgcccag caggccgtag ggaaccgcga tacgcagcat cggcgcgtgg
     6661 cgctggatgt agagcccgtt gcgcaaccgc aggccccgaa attcctcttc cggcagctcc
     6721 ccggcgagaa agcgccgcgt ctgatcccgg aactccgcga cccgctgatc gacgaaggcc
     6781 tgatcgagct catcatagcg atacatgggg ccgactatag gatcgcgggc aggctgcgcc
     6841 gaaataccct ttggttgggt gttcggcgct gccggttatg agcgggtaca atgcccgcaa
     6901 gcgcgtgcga gagctgactc gcaggggatg cgcaaggggt ggcgggtgcc tggactgtat
     6961 tttgaggagt tcgaggtagg gcagcggttc gagcacgtcg tgcatcgcac cgtcacggag
     7021 gcggacaacg ttctcttcag cac
//
LOCUS       DASRHN010000146        10614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1369339, whole genome shotgun sequence.
ACCESSION   DASRHN010000146 DASRHN010000000
VERSION     DASRHN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369339"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..884
                     /gene="choW"
                     /locus_tag="VFB37_05555"
     CDS             9..884
                     /gene="choW"
                     /locus_tag="VFB37_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter permease subunit"
                     /protein_id="HZO21952.1"
                     /translation="MRNRIGAAGFDDWVSGHKIPVGDAVTVVIEYVKTHGRSVFDSVS
                     TGIRGSVNGLTAALSWIPSPILILLLCLIAWVLRRSVALTVFVGLALLFIINQGYWQA
                     TIETLALVIVAALLSTIVGVPLGIAAAHRPRLYATMRPVLDLMQTLPTFVYLIPTLVL
                     FGLGVVPGLISTVIFALPAPIRLTHLGVSSVPKPLIEAGEAFGATHMQMLWKVELPSA
                     APTIIAGITQCIMLSLSMVVIAALVGAGGLGVPVVRALNTVQVGMGFEAGFVIVLLAI
                     ILDRITRPADKSNTP"
     gene            894..2093
                     /gene="choV"
                     /locus_tag="VFB37_05560"
     CDS             894..2093
                     /gene="choV"
                     /locus_tag="VFB37_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015220 - choline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015871 - choline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter ATP-binding protein"
                     /protein_id="HZO21953.1"
                     /translation="MQSALEFRNVDILFGAGSGRRAAALTQQALAALDAGSTRARIAE
                     ETGIIVGVANANLCVEPGEISVLMGLSGSGKSTLLRAANGLTSVTRGQVLVQDSDAVV
                     DIGAGCDAATLRRIRRQRIAMVFQQFGLLPWRTVRENVGFGLELRGEPAAARRRVIDE
                     QLELVGLTQWADRHVGELSGGMQQRVGLARAFATNADVLLMDEPFSALDPLIRRKLQD
                     ELLTLQQRVKKTILFVSHDLDEALKLGDHITILEGGRIVQTGTAEDIIVRPANNYVAE
                     FVTHMNPLSVLTGDMVMRPCAEMIGDDGVLWLDPSRRYQIVLDERGGPGVLRVDGTEQ
                     ALGHVREAEGWSSAQPGMAVAPASISLSAIIRLRNTTGHPVLIVEEGRLAGVCDQPEI
                     IRALAGR"
     gene            2280..3530
                     /locus_tag="VFB37_05565"
     CDS             2280..3530
                     /locus_tag="VFB37_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HZO21954.1"
                     /translation="MQRFSAFTLLRRGLSDQRGWQPQWRRAEPRSGYDVVIVGGGGHG
                     LGTAYYLAREHGIQSIAVLEKGWIGGGNTGRNTTIIRSNYLFDESAGLYDHALKLWES
                     LSQELNYNVMFSPRGVMMLAHTVHDVQVSRRHIHANRANGVDNEWLSPREAQQFCPPL
                     NIGDIRYPVLGAALQRRGGTARHDAVAWGYARAADALGVDVIENCEVTGIRCDANGAV
                     AALDTSRGVIEARRVGVVTAGNTSVVMKMAGVRLPLESYPLQALVSEPVKPVFPCVVM
                     SNTIHAYISQSDKGELVIGAGTDAYNSYTQRGGLHISSHALEAVCELFPPFRRLRMLR
                     NWAGIVDVTPDRSPIIGKTPVPGLYVNCGWGTGGFKATPGSAHVFAHTIAHGEPHPLN
                     APFTIERFAAGNLIDEAAAAAVAH"
     gene            3537..3803
                     /locus_tag="VFB37_05570"
     CDS             3537..3803
                     /locus_tag="VFB37_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HZO21955.1"
                     /translation="MLLIKCPYCGERPELEFAHGGEAHIARPQDPSQAGAQDWANFLY
                     VRNNTKGVYAERWRHAQGCGKFFNALRDTTTDRFVSTYRIGERP"
     gene            3929..6826
                     /locus_tag="VFB37_05575"
     CDS             3929..6826
                     /locus_tag="VFB37_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HZO21956.1"
                     /translation="MHFTFDGRPYIGLRGDTLASALLANGVHLVGRSFKYHRPRGIFS
                     AGAEEPNALVAIRRDAARYAPNLRATQVELYEGLCAESQNRWPGLEFDVGAVNDALSR
                     FIPAGFYYKTFMWPRRAWHALYEPRIRASAGLGQAPDLPDPDRYTNRFAHCDTLVVGA
                     GPAGLSAALAAARSGARIILCDEQSELGGSLLSEPHAGARIEGRPAGDWLRDAIATLA
                     SLPNVRLLSRTTAFGYFPHNLVALNERLTDHLARLPAHMPRERTWQVRAREVILATGA
                     IERPLVFPGNDRPGIMLAGAARTYVNRYGVLPGTVAVVAAAHDEGYRAALDLHAAGVT
                     IAAIVDVREAPSGDLPNAAREAGLPIQPRSTIVGTRGRLRVNSVAVAQIDSHGSVSSG
                     SDSIACDLVAMAGGFTPAAHLYSQSRGQLAWDDQLQAFLPSTAAEQVRAVGACRGMFD
                     LAAVLQGCASVSALNASPQAGSSILPQRADATQKAFVDWQHDVTSRDLALATREGFRS
                     IEHVKRYTTAGMATDQGKTSSMNALAVVSREIGKPVPQVGLTTFRMPYTPVTFGGFAG
                     LQRGSLFDPVRTTPTHQWATEHGAIFEDVGLWKRARYFPRANEDMHAAVARECLAVRN
                     ACGIFDGSTLGKIEVVGKDAVTFMNRMYVNNWSNLAVGRTRYGVLLRDDGFIYDDGVV
                     ARTAEDRFHVTTTTGGAARVLALMEDYAQTEWPELDVWLTSTTEQWAVIAVQGPHSRK
                     VLDTLVENIDLSAKSFPHMSVAHGRICGVPLLLFRVSFTGELGFEINIPADYGQAIWQ
                     AVWQAGQAHGMVPYGTETMHVLRAEKGYIIVGQDTDGTVTPDDAGLAWTIGKSKTDFV
                     GKRSLMRPAMSVADRKQLVGLAPVDPRILLEEGAQLAAVAGQQPPFRPIGHVTSAYYS
                     AFLQRSIALGMVRGGRAALGQVMYVSMPNGDIPVQVTSPVFHDPQGTRLHA"
     gene            6795..7367
                     /locus_tag="VFB37_05580"
     CDS             6795..7367
                     /locus_tag="VFB37_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HZO21957.1"
                     /translation="MTRRGLGCMPDLTHLRTSPVLTASPGVRMLPHATRYVLRGGPQV
                     MLAAGDALGLVIASEPCRSTASAKHAALWLGPDEQLLIGQEADGSDLGSLLREKLAGL
                     PCSIVDISHRQIALEISGPHAQTLLSVGCPLDLDTAGFPRGMCTRTLLGKANVVLWRT
                     QTHTFHLEIWRSFADYVTRFLAEAGRELAC"
     gene            7384..9843
                     /locus_tag="VFB37_05585"
     CDS             7384..9843
                     /locus_tag="VFB37_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO21958.1"
                     /translation="MKTHVRVLVIGGGVVGVSTLYHLGRKGWKDIALIERTELTAGST
                     WHAAGLLPLFNMSYTVGQLHKYSVDLYKRLPAETGQDVSFHVSGNLRLATCRERMDEY
                     QKYCGTANTIGVPFEIIGPARVKELWPLIELGGSSETAPIIGALYHPHDGHIAPADLT
                     MALRKGARNAGAEIYEQTEAQGIERTPSGEWKIRTSKGDIVAEHIVCATGNYARQTGR
                     LLGLNVPAIPVEHQYIVYDESPELKAYRSGGGRELAVLRESDKSYYLREERMGWILGP
                     YERGAPARFADGVPDWFGRSLFPGDLDRLLPHVEAAMRRVPALESCGIKDIVNGPISY
                     TPDGSPLIGPAWDLPNVWLNEGHSFGVTAAGGAGWQLAEWLVEGEPGIDMLAVDPRRF
                     GPYTSKRYLIAKNEETYRNVFTIHFPDEERPEGRPAKTSAVYDKLDRLGAVWGQRYGW
                     ERANWFAPAGVERQDRWSFRRTNYFEHVGNECRLLRERVGIIDLSPFTKHEVSGPGAE
                     SWLDSLVANKVPAKIGRMALCHALTRRGGIRSEFTITRVGTQKFYVVSAGAAERYDAD
                     YLQKCLPQDGSVELRNITTARGCFVVAGPRSRDVLSKLTDASLENNSFPWLTGQTLEI
                     GLAVDVYALRVNFVGALGWELHFPIEYAHHIFDSLLASGREHGIGMVGMRAMESLRME
                     KSYRMWGSDLTTDYTPFEASLDRFVRMNKGAFVGREALEKQLASGVPNRFVTLEVHEV
                     NDADPLGNEPLFEPGGRIVGRATSGYYGHCLRRSLAIGYVQADFARPGTTLGIEILGE
                     RKRATIVPESPYDPQNADLRG"
     gene            complement(9912..>10614)
                     /locus_tag="VFB37_05590"
     CDS             complement(9912..>10614)
                     /locus_tag="VFB37_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZO21959.1"
                     /translation="RRVVVTVALALVIIFGLVSEVGSVATYAGLITAGLAVALQNVIL
                     AVVAYFFLIGRYGVRVGDRITLAGVTGRVVEIGLIRIYLMELTGPELRSSGRMVVLSN
                     AVLFQPTALFKQMPGGDYFWHAILMTLAPATDVQLAQTRLGEAADSVYEHYRPAIEQQ
                     HAALQRFIDFETAMPRPEVYLQLSEKGLECTVRYPVETARSASIDQKMLSALREARAK
                     APELQLVAGPFLHTT"
BASE COUNT         1818 a         3488 c         3428 g         1880 t
ORIGIN      
        1 tgcgcgggat gcgcaaccga atcggggctg cgggcttcga tgactgggtg agcggccaca
       61 agatccccgt gggcgatgcg gtcaccgtcg tcatcgagta cgtcaagaca cacggccggt
      121 ccgtctttga cagtgtctcg acggggattc gcggcagcgt caacggcctc actgccgcgc
      181 tgagctggat tccatccccg attctcattt tgcttctatg cctcatcgcc tgggtcctgc
      241 gccgctcggt ggccctcacg gtcttcgtgg ggctcgcgct gctgttcatc atcaaccagg
      301 gttactggca ggccacgatc gagacgctcg cgctggtgat cgtcgccgcg ctgctatcga
      361 cgatcgtggg cgttccgctc gggatcgccg ccgcgcatcg gccccgcctg tacgccacca
      421 tgcgtcccgt gctggacctc atgcagacgc tgccgacctt cgtatatctg attcccaccc
      481 tggtcctgtt tgggctcggg gtggtcccgg gattgatctc cacggtcatc ttcgcgctgc
      541 ccgcccccat tcgcctcacg catcttggcg tcagctccgt acccaagccg ctcatcgagg
      601 ccggcgaagc ctttggcgcc acgcacatgc agatgctgtg gaaggtggag ttgcccagcg
      661 ccgcgcccac gatcatcgcc gggatcaccc agtgcatcat gctcagtctc tcgatggtcg
      721 tcatcgctgc cctggtcggc gccggcggcc tgggcgtgcc ggtcgtcagg gcgctcaaca
      781 cggtccaggt cggcatgggg ttcgaggcgg gcttcgtgat cgtgctgctt gccatcatcc
      841 tggatcgcat cacgcgtcct gcggataaat ccaacacacc ctgagacgtg cgcgtgcaat
      901 cagccctaga attccgcaat gtggacattc tgttcggcgc cggctcaggg cggcgcgcgg
      961 ccgcgctgac tcagcaggcg ctcgccgcac tcgatgcggg aagcacacgg gcgcgcatcg
     1021 ccgaggaaac cgggatcatc gtcggcgtcg cgaacgccaa cctgtgcgtc gagcccggcg
     1081 agatctcggt gttgatgggt ctgtcgggat cgggaaaatc cacactatta cgcgcggcca
     1141 atggtctcac tagcgtcacc cgcggccagg tgctcgtgca agacagcgac gccgtggtgg
     1201 acattggtgc cggatgtgat gcggcgaccc tgcgacgaat ccgccgccag cgcatcgcga
     1261 tggtctttca gcaattcgga ctgctaccct ggcgcacggt tcgcgagaac gtcggcttcg
     1321 gtctcgagtt gcggggcgag ccggcggccg cgcgcaggcg cgtcatcgac gagcagctgg
     1381 agctcgtcgg gctgactcag tgggcagacc gtcatgtcgg agagctgtcg ggcggaatgc
     1441 agcagcgtgt cggtctggcg cgggcattcg caaccaacgc cgatgtgctg ctgatggacg
     1501 agcccttctc ggcactcgac ccgctcattc gccgcaagct tcaggatgag ctgctgacgc
     1561 tgcagcagcg cgtcaagaaa accatcctgt tcgtcagcca cgacctcgac gaagcgctca
     1621 agctggggga tcacatcacg atcctcgaag gcggccgcat cgtgcagacg gggaccgcgg
     1681 aggacatcat cgtgcggccc gcaaacaact atgtggcgga gtttgtcacg catatgaacc
     1741 cgctcagcgt gctgaccggg gacatggtca tgcgaccttg cgcggagatg atcggcgatg
     1801 acggggtgct ctggctggac ccgagccgcc gctaccagat cgtcctcgat gagcgtgggg
     1861 gtcccggcgt gctgcgcgtg gatggcaccg agcaggcgct agggcatgtt cgcgaggctg
     1921 aaggttggtc gtcggcgcag cccggcatgg ccgtggctcc cgcctcaatc tccttgagcg
     1981 ccatcatccg gctccgcaat acaaccgggc acccggtgct gatcgtcgag gagggccgcc
     2041 tcgccggggt gtgtgaccag ccggagatca tccgcgcgct cgcaggacgc tgaaagcccc
     2101 agcctcgctg gccgtcgtgt ccggccgtaa ccggcccggt tcggcctcat aaaaaaaccg
     2161 ccgtctgatg tcgctttcgt tcaaatggcg cggcgagcgc atgctcgtgc ccgctccggc
     2221 cgcccggcat gatgccggcg ccctactcca gggatcgctg atgccccgcc aagatccgga
     2281 tgcaacgctt ctccgccttc acccttcttc gccgcgggct gtccgatcag cgcggctggc
     2341 agccgcaatg gcgccgcgct gagccacgct ccggatacga tgtcgtgatc gtggggggcg
     2401 gcggccatgg gttaggcacc gcttattacc tcgcccgcga gcacgggatc cagagcatcg
     2461 ccgtgctcga gaagggctgg atcggcgggg gtaacaccgg tcgcaacacg accatcatcc
     2521 gttcgaacta tctgttcgat gagagcgcgg gcttgtacga ccatgcgctc aaattgtggg
     2581 agagcctctc gcaagagctc aactacaacg tcatgttctc tccccgtggc gtcatgatgc
     2641 tcgcccacac ggtccatgat gtacaggtct cccgacgcca tatccacgct aaccgcgcca
     2701 atggagtgga caacgagtgg ctgagccccc gtgaggccca gcagttctgt ccaccgctga
     2761 acatcggcga catccgctat ccagtactcg gtgccgcttt gcagagacga ggcggcacgg
     2821 ctcggcacga tgcggtcgca tggggttatg cccgcgcagc cgacgctctg ggcgtggatg
     2881 tcatcgagaa ctgcgaggtc acggggatcc gctgcgatgc caatggcgcg gtcgcggcac
     2941 tggataccag ccgtggagtg attgaagcac gccgcgtcgg cgtcgtgacc gcgggaaaca
     3001 ccagtgtggt gatgaagatg gccggggtac ggctgccact ggaaagctac ccgctccagg
     3061 cactggtctc cgagccggtc aagcctgtct tcccgtgcgt cgtcatgtcc aacaccattc
     3121 atgcctacat cagtcagtcg gacaaaggag agttggtgat cggcgccggc acggatgcct
     3181 acaattccta tacgcagcgg ggtggcctgc acatcagcag tcacgcactc gaggcagtct
     3241 gcgagctctt tccgccattc cgacgcctgc ggatgctgcg caactgggcg ggcatcgtcg
     3301 atgtgacccc ggatcgctcg cccatcattg gcaagactcc cgtgcccgga ctgtatgtaa
     3361 attgcggctg gggcacgggc gggttcaaag cgactccggg atcggctcac gtgttcgcac
     3421 atacgatcgc acacggcgag ccgcatccgc tcaacgctcc cttcacgatc gaacgattcg
     3481 ccgccggtaa cctgatcgat gaagccgctg cggccgcggt cgcgcactga ggctgcatgc
     3541 tgctcatcaa atgcccctat tgcggcgagc gtcccgagct cgaattcgcc cacggtggcg
     3601 aggcacacat cgctcgcccg caggaccctt cccaggccgg agcacaggac tgggcgaact
     3661 ttctttatgt gcgaaacaac accaaagggg tgtacgccga gcgctggaga cacgcgcagg
     3721 ggtgcggcaa gttcttcaac gccctgcgcg atacgaccac ggaccgcttc gtgagcactt
     3781 acagaatcgg tgagcggccg tgagcgtggg cgcgcagcgg ctcgtcgtcg aaggtgtggg
     3841 acgcgacccg caaagagatg cgcctgatct tgcagtcgga ggcgccttcc gtgtctctcg
     3901 cggtggtcgc atcgaacgcg ggcagccctt gcattttacc ttcgacggcc gcccctacat
     3961 cggattacgc ggagacactc tggcctctgc gctcctcgcg aatggcgtgc acctcgtcgg
     4021 ccgttccttc aagtatcacc gcccgcgcgg catcttttcc gctggtgcgg aagagcccaa
     4081 cgctctggtg gctatccggc gcgatgcggc acgctacgcc cccaatctgc gagcgaccca
     4141 ggtggagctt tacgaggggc tatgcgctga gagtcagaac cgctggccgg ggctggaatt
     4201 cgatgtgggc gccgtgaacg atgcgctgtc tcggttcatt cctgccggct tctactacaa
     4261 gactttcatg tggccgcgcc gcgcgtggca tgcactctac gagccgcgca tccgcgcctc
     4321 tgcgggcctg ggacaggccc ctgatcttcc tgatcccgac cgctacacga accgcttcgc
     4381 acattgcgac acgctggtcg ttggcgcagg tcctgcgggc ctctctgcgg ctcttgccgc
     4441 ggcgcgctcc ggtgcgcgca taatcttgtg cgacgagcag tcggaactgg gcggcagcct
     4501 cctcagcgag ccgcacgccg gggcacgcat cgaggggcgg cccgccggcg attggctgcg
     4561 cgatgcgatc gcgacgctcg cgagcctgcc taatgtgcgg ctgctctccc gaaccacggc
     4621 gttcggttac tttccgcaca acctggtcgc cttgaacgag cggctcaccg atcacctcgc
     4681 aaggctccct gctcatatgc cgcgagagcg cacctggcag gtgcgcgccc gggaagtcat
     4741 cctcgccacg ggagccatcg agcggccgct ggtgtttccg ggcaacgacc gccccggaat
     4801 catgctggca ggcgccgccc gcacctacgt caaccggtat ggggtgcttc cgggtacggt
     4861 tgccgtcgtc gccgctgcgc atgacgaggg gtatcgcgcg gcactcgatc tgcatgcagc
     4921 cggcgtcacc atcgccgcga tagtggacgt gcgcgaggcg ccgagcggag atctgccgaa
     4981 tgccgctcgt gaggcgggcc tgcctattca gccgcgctcg accatcgtgg gcacgagggg
     5041 ccgcttgcgt gtcaactcgg tagcggtcgc ccagatcgac agtcacggca gcgtctccag
     5101 tggctcggac tcgattgcct gcgatctcgt ggcgatggcg ggcgggttca cccccgccgc
     5161 gcacttgtat tcccagtcgc gcggccaact cgcctgggac gaccaactgc aggcctttct
     5221 cccttctact gcggccgagc aggtgcgcgc ggtgggggca tgtcggggaa tgttcgatct
     5281 ggccgcggtg ctgcagggct gcgcttcggt cagcgctctg aatgcttcgc cgcaggccgg
     5341 ctcgagcatt cttccgcagc gcgcggatgc gactcaaaag gcgttcgtgg actggcaaca
     5401 cgatgtaacg agccgtgacc tcgcgctcgc cactcgtgag ggatttcgct cgatcgagca
     5461 tgtcaaacgg tacaccaccg cgggcatggc gaccgatcaa ggcaagacct ccagcatgaa
     5521 cgccctggcg gtcgtctcgc gcgagatcgg caaacctgtt ccgcaggtgg gcctcaccac
     5581 ctttcgcatg ccctacacgc cggtgacgtt cggcggcttt gcaggactgc agcgcggaag
     5641 cctcttcgat cccgtgcgca ccacgccgac gcatcaatgg gcaaccgagc atggggccat
     5701 attcgaggat gtcgggctct ggaagcgggc ccgctatttt ccgcgagcga acgaggatat
     5761 gcacgcagcg gtcgcgcgcg agtgtctggc cgtgcgcaac gcctgtggaa tctttgatgg
     5821 ctcgacactc ggcaagatcg aagtcgtggg caaggacgcc gtgacgttca tgaaccgcat
     5881 gtatgtcaac aactggtcga atctcgccgt cggtcgaacc cgatacggcg tgctcctccg
     5941 ggacgacgga ttcatctacg acgacggcgt tgtcgcgaga accgctgaag atcgattcca
     6001 tgtgaccacc acgacagggg gtgccgcacg tgtgcttgcg ctcatggaag actatgctca
     6061 gaccgagtgg ccagagctgg acgtctggct cacctcgacg accgaacagt gggccgtcat
     6121 cgcggtgcaa ggaccccact cgcgcaaggt gctggatacg ctggtggaga acatcgacct
     6181 ttctgcgaag tccttccccc acatgagcgt cgcgcacggc aggatctgcg gcgtgccgct
     6241 gcttcttttc agagtcagct tcaccggcga gctcggcttc gagatcaata tcccggccga
     6301 ttatggccag gccatctggc aggcggtctg gcaagccgga caagcgcacg gcatggtccc
     6361 ttacggcacc gagacgatgc acgtgctgcg cgccgagaag ggctacatca tcgtcgggca
     6421 ggatactgat ggcaccgtca cgccggatga tgccgggctc gcctggacga tcggcaagag
     6481 caagacggac ttcgtgggca agcgctcact gatgcgtccc gcgatgagcg tcgccgatcg
     6541 gaagcagctc gtggggctcg ctccggtcga tccgcgcatc ctgctcgaag aaggcgcgca
     6601 gctcgcagcg gtggcgggcc agcagccgcc cttccggccg atcggacatg tgacttccgc
     6661 ttactacagc gcctttctgc agcgctcgat tgcgctcggc atggtgcgcg gcgggcgggc
     6721 ggcgctcggc caggtcatgt atgtgtccat gccgaacggc gatattccgg tacaggtcac
     6781 ctctccggtg tttcatgacc cgcaggggac tcggctgcat gcctgatctg acccatctgc
     6841 gcacttcccc agtgctcacc gccagcccgg gcgttcgcat gctgccgcac gccactcggt
     6901 atgttttgcg cggcggtcca caagtcatgc tcgccgccgg cgatgcgctc ggactcgtca
     6961 ttgccagtga accgtgccgc agcacggcca gcgcaaagca tgcggcgctg tggctcggcc
     7021 ccgatgagca gctgctgatc gggcaggagg ccgatgggtc cgacctcggc tccttgcttc
     7081 gcgaaaaact cgccggcctg ccctgctcca tcgtggatat cagccatcgg cagattgcgc
     7141 tcgagatcag cgggccgcac gcgcagactt tgctcagcgt cggctgtccg ctggacctcg
     7201 acacggccgg atttccccgc ggtatgtgca cgcggaccct gcttggcaag gcgaacgtcg
     7261 tcctctggcg cacgcaaaca cacaccttcc atctcgagat ctggcgctct ttcgccgact
     7321 acgtgacgcg cttcctcgcc gaggccggac gcgagctcgc ctgttgaacc tggagaaatc
     7381 tctgtgaaga cgcatgtcag ggtgttggtc atcggcggcg gtgtggtcgg ggtgagtacc
     7441 ctgtatcacc tgggcagaaa gggctggaag gacatcgccc tgatcgagcg cacggagctc
     7501 acggcaggtt ccacctggca tgccgccggg ctgctgccgc tgttcaacat gagctatacg
     7561 gtcggtcagc tgcacaaata ctcggtggac ctctataaac gcctgccggc ggagaccggc
     7621 caggacgtca gcttccacgt gagcggcaat ctgcgcctcg ccacctgccg cgagcgcatg
     7681 gacgagtatc agaagtactg tggcacagcc aacaccatcg gcgtcccgtt cgagatcatc
     7741 ggaccggcgc gcgtcaagga gctgtggccg ctgatcgagc tcggcggcag ctccgagact
     7801 gcgcccatca ttggcgccct gtatcatccg catgacggtc acatcgcccc ggccgacctg
     7861 accatggctc tgcgcaaggg agcccgcaac gcgggggccg agatctacga gcagaccgag
     7921 gcgcagggca tcgagcgcac gccttccggc gagtggaaaa tccgaacttc caagggtgac
     7981 atcgtcgccg agcacatcgt ctgcgccacc ggaaactatg cccgtcagac cggccgcctc
     8041 ctcgggctca acgtgccggc cattcccgtc gagcaccagt acatcgtcta tgacgaatcg
     8101 cctgagctga aggcctatcg cagcggtggt ggccgcgagc ttgccgtcct gcgcgagtcc
     8161 gacaagtcct actacctgcg cgaggagcgc atgggatgga tcctgggtcc atacgagaga
     8221 ggcgcaccgg cgcgcttcgc ggatggcgtg ccggactggt ttggccgcag cctcttcccc
     8281 ggtgatctcg accggctgct gccgcacgtc gaggccgcga tgcggcgcgt cccggcactg
     8341 gagagctgcg ggataaagga catcgtgaac ggtcccatct cctacacgcc cgacggcagc
     8401 ccgctcatcg gtccggcctg ggatctgccc aacgtgtggc tgaacgaagg gcacagcttc
     8461 ggtgtcacgg ctgcgggcgg cgcgggctgg cagctcgccg aatggctggt cgaaggcgag
     8521 cccggtatcg acatgctggc agtggatccg cgccgcttcg gaccctacac cagcaagcgc
     8581 tatctgatcg cgaagaacga ggagacctac cgcaacgtct tcacgattca ttttcccgat
     8641 gaggagcgcc ccgaggggcg tcccgcgaag accagcgcgg tctatgacaa gctcgaccgt
     8701 ctgggcgcgg tctggggcca gcgctacggc tgggagcggg cgaattggtt cgcgcccgcc
     8761 ggagtggagc gtcaggatcg ctggagcttt cgccgcacaa actacttcga gcacgtcggc
     8821 aacgagtgcc gcctgctgcg cgagcgcgtc ggcatcatcg atctcagccc gttcaccaag
     8881 catgaagtca gcggcccggg cgctgaaagc tggctcgaca gcctggtcgc caacaaggtg
     8941 ccggcaaaga tcggccgcat ggccctgtgt cacgcgctga cacgccgcgg tggcatccgc
     9001 tccgaattca cgatcacccg ggtgggcacc cagaagttct atgtcgtgag cgccggagct
     9061 gccgagcgct acgacgccga ttacctgcag aagtgcttgc cgcaggacgg ctcggtggaa
     9121 ctgcgcaaca tcaccaccgc acgcggctgc ttcgtagtgg cgggaccgcg ctcacgcgat
     9181 gtgctctcga agctgacgga tgcctccctc gagaacaact cgttcccgtg gctgaccgga
     9241 cagacgctcg agatcggcct ggcagtggat gtgtacgcgc tgcgtgtcaa cttcgtcggt
     9301 gcgctcggct gggagctgca tttcccgatc gagtacgccc atcatatttt cgactccttg
     9361 ctcgccagcg ggcgcgagca cggcatcggc atggtaggca tgcgcgcgat ggagtcgctc
     9421 cgcatggaga agtcctatcg catgtggggc agcgatctga caacggatta cacgcctttc
     9481 gaggccagcc tcgatcggtt cgtgcgcatg aacaagggcg cattcgtggg ccgggaggca
     9541 ttggaaaaac agctggcctc gggcgtcccg aaccggttcg tgaccctcga ggtgcatgag
     9601 gtgaacgatg ccgatccgct cggcaacgag ccgctcttcg agccgggggg ccgcatcgtg
     9661 ggacgggcaa catccgggta ctacgggcat tgcctgcgca gaagcctcgc gatcggctac
     9721 gtccaggctg acttcgctcg cccgggcacc acgctcggaa tcgagattct gggcgagcgc
     9781 aagcgggcca cgatcgttcc ggagtcgccg tacgatcccc agaacgcgga ccttcgcggc
     9841 tgagtttcga atcgcgccag cttcaggccc gtgggtcagg aagggccact catcccgggc
     9901 agaaactgca gctaagtcgt gtgcaggaac gggccggcta cgagctgcag ctctggcgcc
     9961 ttggcgcgtg cctccctcaa ggcactcagc atcttctgat cgatgctggc tgagcgcgcc
    10021 gtctcgaccg gatagcgcac cgtgcattcg agacccttct ccgagagttg caggtagacc
    10081 tccggccgcg gcatcgccgt ctcgaagtcg atgaagcgct gcaaggcggc gtgctgctgc
    10141 tcgatcgcgg ggcgatagtg ctcatacacg gagtctgccg cctccccgag ccgggtctgc
    10201 gcgagctgca catccgttgc gggcgcgagc gtcatgagga tcgcgtgcca gaaatagtcc
    10261 ccgcccggca tctgcttgaa gagcgccgtc ggctgaaaga gcaccgcgtt ggagagcacg
    10321 accatgcgac ccgaggagcg cagctccggg ccggtcagct ccatcaggta gatgcggatc
    10381 aggccaatct ccaccacccg ccccgtcacg cccgcaagcg tgatccggtc gccgacgcgc
    10441 accccataac ggccaatgag aaagaagtag gccacgacgg cgaggatcac attctgcagc
    10501 gcgacggcca gcccggccgt gatgagcccg gcatacgtgg cgaccgagcc cacttccgag
    10561 acgaggccga agatgatcac cagcgcgagc gccacggtga ccaccacccg gcga
//
LOCUS       DASRHN010000147         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_540856, whole genome shotgun sequence.
ACCESSION   DASRHN010000147 DASRHN010000000
VERSION     DASRHN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540856"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFB37_05595"
     CDS             complement(<1..698)
                     /locus_tag="VFB37_05595"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HZO21960.1"
                     /translation="MRIAMIGLGRMGANMVRRLQQGGHECIAYDPSADAVKTVVGYGA
                     RGAKSLAELVEALKPPRVVWVMVPASVVDGVLKELRALLKSGDVIIDGGNSHYHDDIR
                     RADELQPAGIHYVDVGTSGGVLGFEHGFSLMIGGEKDVVASIEPVFATLSPGGKPPER
                     AKGSGSAAPTPAQGTAGKGYLHCGPNGAGHFVKMVHNGIEYGLMAAYAEGLNLLKHAD
                     AGLQSRESDAETAPL"
     gene            complement(770..2029)
                     /gene="murA"
                     /locus_tag="VFB37_05600"
     CDS             complement(770..2029)
                     /gene="murA"
                     /locus_tag="VFB37_05600"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZO21961.1"
                     /translation="MDKLQIQGGVPLEGEVRISGAKNATLPILAGALLADDPVVVANV
                     PHLKDVTTTVELLGRMGATVTIDERMRIEVDGSTLKECFAPYDLVKTMRASILVLGPL
                     VARFGRADVSLPGGCAIGARPVNIHVAGLQAMGADITIEGGYIRARADRLKGARLVLD
                     TVTVTGTENLMMAATLAEGETVIENAAREPEVVDLANFLIAMGAQIQGAGTDKIVVQG
                     VERLRGTTYEVLPDRIESGTYLVAGAITRGHVRVKNARPDHLDAVLAKLEEAGATVGV
                     GDNWIEVDMRGRRPQAVDVRTAPYPAFPTDMQAQFAALNAVAAGVGTIIETIFENRFM
                     HMLEMRRLGAEIRLEGNTAIIRGVDRLTAAPVMATDLRASASLVLAGLVAEGRTDIER
                     IYHIDRGYEAIEEKLAQLGAQIRRVPN"
     gene            complement(2031..2267)
                     /locus_tag="VFB37_05605"
     CDS             complement(2031..2267)
                     /locus_tag="VFB37_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZO21962.1"
                     /translation="MSPDQVAQLIRTALPDAQVLVQSDDNTHFAARIVSREFAGKRSL
                     ARHQLVYRALGERMGGEIHALSIEALTPEESAAG"
     gene            2319..3323
                     /locus_tag="VFB37_05610"
     CDS             2319..3323
                     /locus_tag="VFB37_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZO21963.1"
                     /translation="MQSPAPIAPQTLDDQQLLASARRALAIEAQAVAALAPRLDAAFP
                     AACRICLGCQGRIVVTGIGKSGHIARKIAATLASTGTPAFFLHPAEAGHGDLGMITRT
                     DVLLAISNSGETPELVLLLPHLKRLAVPLIVIVGKVDSTLGRCAAVCLDVSVPEEACP
                     LNLAPTASTTAMLAMGDALAVSILEARGFTKQDFARSHPGGSLGRQLLLHVEDMMRTG
                     AAIPRVGPDDPLGQGLIEMSRKALGMTIVVDPTDHILGIFTDGDLRRALDKRIDVHQT
                     PMSAVMTRGCKTIGPRALAAEAVHLMELHRITALPVADSENRIIGALNVHDLFRAGVV
                     "
BASE COUNT          578 a         1214 c         1103 g          616 t
ORIGIN      
        1 agcggtgcgg tctcggcatc cgactcgcgg ctttgcaggc ccgcatctgc atgcttcagc
       61 agattcaatc cttccgcgta ggccgccatc aggccgtact cgatgccgtt gtgcaccatc
      121 ttgacgaaat gccccgcgcc gttcggcccg cagtgcagat aacccttgcc cgcagtgccc
      181 tgcgcaggcg tcggcgctgc ggagccgctg cccttggcac gctccggagg tttgccgccg
      241 ggagagagcg tcgcgaagac cggctcgatg gaagcgacca cgtctttctc gccgccgatc
      301 atcaggctga agccgtgctc gaacccgagc acgcccccgc tcgtgccgac atccacgtag
      361 tgaattccag ctggctgcag ctcatcggcg cgccggatgt cgtcgtgata atgggaattg
      421 ccgccgtcga tgatgacgtc gccggacttc aacagcgcgc gaagctcctt gagcacgccg
      481 tcgacgacac tcgcgggcac catcacccac acgacgcgcg ggggtttcag cgcctcgacg
      541 agctccgcga gcgacttggc tccacgcgct ccgtagccca cgaccgtctt gactgcgtcc
      601 gccgacgggt cgtaggcgat gcactcgtga ccgccctgtt gcagcctgcg caccatgttg
      661 gcgcccatac gcccaagccc gatcatcgcg atgcgcatat gcactccagg ggttgaacgg
      721 ccgattctac gcgctgcaga tgtctgccgg ctgtaagacg gatccgacgc taattgggaa
      781 cgcgcctgat ctgtgccccg agctgggcca gcttttcctc gatcgcctcg tagccgcgat
      841 cgatgtggta gatacgctcg atatccgtgc gcccctcggc caccagcccg gcgagcacca
      901 ggctggcgga ggcacgcaga tcggttgcca tgaccggcgc ggccgtgagt cgatcgacgc
      961 cgcgaatgat tgcggtgttg ccctcgagcc ggatctccgc gccgagccgg cgcatctcga
     1021 gcatgtgcat gaaccggttc tcgaagatgg tctcgatgat cgtaccgacg ccggcggcca
     1081 ccgcgttcaa ggcagcaaat tgcgcctgca tgtcggttgg aaacgccgga tagggggcag
     1141 tgcgcacatc gacggcctgc ggacgacggc cgcgcatgtc cacctcgatc cagttatcgc
     1201 cgacgccaac cgtggcgccg gcttcctcga gcttggccag taccgcatcc agatgatccg
     1261 gacgcgcatt cttgacgcgc acgtggcccc gcgtgatcgc gccggccacg aggtaggtcc
     1321 cgctctcgat gcgatccgga aggacttcgt aggtcgttcc ccggagccgc tccacaccct
     1381 ggacgactat tttgtcggtt ccggcacctt ggatctgtgc gcccatggcg atcaggaagt
     1441 tcgcgaggtc cacgacctcg ggctcgcgcg cggcgttctc gattaccgtc tcgccctcgg
     1501 caagcgtggc cgccatcatg agattctcgg tcccggtgac cgtgaccgta tcgagtacca
     1561 ggcgcgcgcc cttgaggcga tcggcgcgcg cgcggatata gcctccctcg atggtgatgt
     1621 cggcgcccat cgcctgaagg cccgccacgt ggatgttcac cgggcgcgca ccaatggcgc
     1681 agcctcccgg cagtgacaca tcagcccgtc cgaagcgcgc caccagagga cccagcacga
     1741 ggatcgatgc gcgcatggtc ttcacgagat cgtagggtgc gaagcactcc ttcaaggtcg
     1801 agccgtccac ctcgatccgc atccgctcat cgatcgtcac ggtcgccccc atgcgcccga
     1861 ggagctcgac ggtcgtggtg acgtccttca gatgcgggac attggccacg acgaccggat
     1921 catccgccag aagcgccccc gccaggatgg gcagggtggc gttcttcgcg cctgagatcc
     1981 gcacttcacc ttcgaggggc acgcccccct ggatctgcag cttgtccatg tcaccccgca
     2041 gcggattctt cgggcgtcag cgcttcgatc gacagggcat ggatctcacc gcccatgcgc
     2101 tcgcccagcg cgcggtagac gagctgatgg cgtgcgaggc tgcgcttgcc ggcaaactcg
     2161 cgcgacacga tccgtgcggc gaaatgggtg ttgtcgtccg attggacgag gacctgcgca
     2221 tcgggaagcg cggtgcggat gagctgtgca acctgatcgg gactcatggg ccaagatatt
     2281 aatggctggg cagctgccgc gcctataatc ccagccgcat gcagtccccg gccccgattg
     2341 ccccccagac cctggacgac cagcagcttc tcgccagcgc ccggcgggcg ctggccatcg
     2401 aagcccaggc cgtcgcagct cttgcacccc gactcgatgc cgcctttcct gcggcctgcc
     2461 ggatttgtct cggctgccag ggccggatcg tcgtcacggg gatcggcaag tccggacaca
     2521 tcgcacgcaa gatcgcagcc accctcgcga gcaccggcac gccggcgttc ttcctccatc
     2581 cggccgaggc cggccatgga gatctgggca tgatcacgcg caccgatgtg ctgctggcga
     2641 tttcaaactc cggcgagaca cccgagctcg tgcttctgct gccgcatctc aagcggctcg
     2701 ccgtgccgct gatcgtgatc gtgggcaagg tggattcgac cctcggacgc tgcgccgccg
     2761 tgtgcctcga tgtgagtgtt ccggaggagg cctgcccgct caacctggcc ccgaccgcca
     2821 gcaccacggc gatgctggcg atgggagatg cactcgccgt atccatactg gaggctcgcg
     2881 gcttcaccaa gcaggacttc gcgcgatcgc accctggcgg gagcctcggc cgccagctcc
     2941 tgctgcatgt cgaggacatg atgcgcaccg gcgcggccat cccgcgggtc ggcccggacg
     3001 atccgctggg acagggcttg atcgagatgt cacgcaaagc tctgggcatg acaatagtcg
     3061 tcgacccgac cgaccacatc ctgggcatct tcaccgatgg cgacctgcga agagcattgg
     3121 acaagcgcat cgacgtgcac cagacgccga tgagcgccgt gatgacccgc ggctgcaaga
     3181 cgatcggtcc gcgggcgctc gccgctgaag ccgtgcacct gatggagctg caccgcatca
     3241 cggcgctgcc cgttgccgac tccgagaacc gcatcatcgg cgccttgaac gttcacgatc
     3301 ttttccgtgc cggcgtggtc tgagtaggca ggcgccgcgc cagcttcgct ctcgtgcacc
     3361 gcatgactcg caccatggcc gctcgcatcc aactcctggt cctcgatgtg gacggggtca
     3421 tgacggacgg ccggctttac tacggaccgc ggggtgaggc gctcaagatt ttccatgtcc
     3481 gggacggcaa gggtattcag ctgctggatg c
//
LOCUS       DASRHN010000148        45999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1036821, whole genome shotgun sequence.
ACCESSION   DASRHN010000148 DASRHN010000000
VERSION     DASRHN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 45999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45999
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036821"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VFB37_05615"
     CDS             <1..1439
                     /locus_tag="VFB37_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZO21964.1"
                     /translation="FIYQGFNTRVGWMHTSSGVDNVDEFAETIVTAPDGSRAYRYGKE
                     LRPLTSKQISLSYRAANGSMATRTFTTYATHHGPIVREEGGKWIAFALMNRPIAALEQ
                     SFLRTKAKDYASYLKVAQLRANSSNNTIFADSKGEIAYLHPQFVPVRDDRFDYRAPVD
                     GSDPATDWKGLHSLESLPHVVSPANGWVMNTNDAPWTAAGADSPKAADYPRYMDEAGE
                     DPRGPHAIRVLTARQDFTPQTLIAAAFDPYLTEFAQLIPLLLKAFDALPQSDPGRTTL
                     AGPIKLLRDWDYRWGLTSQPTSLAVFWGDALWHVCAEPAKAARLSVWDYMAEHTSDAQ
                     KLAALTEASQRLTHDFGSYAVPWGEINRFQRNDGAIVQTFSDVKASIPVPFTSSRWGS
                     LASFAAKRYPGTKCYYGTEGNSFVAVVEFGPRVRAWGITAGGESGDPRSVHFLDEAQR
                     YADGNLRPIYFYPGDLKGHTEKRYHPGE"
     gene            complement(1448..3511)
                     /locus_tag="VFB37_05620"
     CDS             complement(1448..3511)
                     /locus_tag="VFB37_05620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO21965.1"
                     /translation="MAGKTLAEGKLMNERAMGLARLAAAGLCCVLSEIAGAESPSIAK
                     AVEALTSVHPIAEVALSPDGKRVIYGSVVTAKRGGADVASSMLWMANARNGSAAVRLT
                     ACPSMECDEHSAAWSPDGRQVAFVTTDSNGQSQLAIASETGSDAKVLTNAHGPLDTPR
                     WSPDGKQIAFLYSEGAPRTPGPLNPLARDAGVLSETIYEQRLALISATGGEIHLLGPG
                     NLNIYEYDWAPDSKRFVVSAAPGSGDDNWWIAELQLIDTQTGSTTSLLKPKLQIASPR
                     WSSDGAQIAYISGIMSDEGITGGDVYLVPASGGTPVDLTPDLRASVQTITWNGSSSHI
                     VATEFSAGDEVLASIDVKAKSQREIWRGPQMIWANSLLGFTPGDAGISLSRDAAMSAI
                     VRQSYTMAPEVETGTPGKWKPITRLNAAVHSITGNAKIVTWKSDRFDVQGMLIEPAVP
                     GGAGKHYPLVVEVHGGPAFAHYPLFPASPDSFDAVLSSQGYYVFKPNPRGSYGQGEAF
                     TQANVKDFGYGDLRDILRGLDTVEKSDPIDAARVGIAGWSYGGYMTMWALTQTDRFKA
                     AVSGAGLSDWLSYYGTNNIDTWMIPYFGASVYDDPKVYERSSPITFIKKVKTPTLVVG
                     GDRDAEVPITQSYEYWNALKRLGVKTEFVVYPDEGHFFFKRADQIDVMTRLVDWFNQY
                     LQGTP"
     gene            3674..5335
                     /locus_tag="VFB37_05625"
     CDS             3674..5335
                     /locus_tag="VFB37_05625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZO21966.1"
                     /translation="MGLLPAAAAAAGGHAELFVNGNIYTADEHNSHAQALVSEAGRII
                     YVGTNSGARQHAPKDSETLDLHGLTVFPGLTDSHAHLRDIGFRELSFNLEGTRSLAEL
                     QRRLRERARTVKGGAWITGGGWIESRWSPPAFPTRADLDQVVNDRPVVLERADGHAIV
                     VNSIALERAGIDRNTPDPPGGRILKEPGGEPNGMLIDNAMELVQHLVPAPTDEQTSKA
                     LQAGAERSVRLGWTQLQIAGNSFHEVDLICQLYAQHLIKLRLYDAIYGPSDDAQRLLR
                     EGPSIDGCGGRITVRGIKLYIDGALGSRGAALLEPYTDAPDSQGLLVNQPSALQPILI
                     AALKRGIQIETHAIGDRGNRIMLDLYQKAFATVPVAQRAIAQPRWRIEHAQILSPADI
                     PRFAQLGVIASMQPSHAISDFFFAPKRLGTERLVGAYAWRSLLDSGATVVAGTDAPVE
                     KGDPIIEFYAAVSRRSLEGFADENWHLEQRVSRAQALRMLSIGPAYAAFQEKERGSIE
                     VGKMTDLSIFSADLMQIPEREILKAHLVMTVIGGEKVYEAQPAKE"
     gene            complement(5431..6030)
                     /locus_tag="VFB37_05630"
     CDS             complement(5431..6030)
                     /locus_tag="VFB37_05630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO21967.1"
                     /translation="MRKRLLACVLASVISLPVLAALKEGDQAPEFKAQASFAGKAFHY
                     SLKDALKKGPVVVYFYPSAYTNGCNIQAHTFAVNSDKFKAAGASIIGVSLDSIKRLND
                     FSADPNYCAGKFPTASDADGKIAKSFELKVMEGKPGLKDTRGVEIDHGFAERTTFIVK
                     PDGTIAATIGGMAPAENVDKALAAVQQLSDDKNGKRAGG"
     gene            6245..7069
                     /locus_tag="VFB37_05635"
     CDS             6245..7069
                     /locus_tag="VFB37_05635"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HZO21968.1"
                     /translation="MPSRVPEGAKRGWIVPIGGAENKENDRRILERFVQLSGGESADI
                     VVIPTASQLYETGARYEQIFSEIGASRVSVMDFDTRRDCQERGRLQRLEEASGIFFTG
                     GNQLRVTTLLGGTPVAKLIRARNANGVTVGGTSAGASILSEHMIASGDEGSAAIAGSV
                     RLAPGLGLTNRFIIDQHFRQRDRLGRLITALAYNPFAVGIGLDEDTAALIGPDDVLEV
                     EGSGSVTVVDGADVTYSSIHEVSDGQPICMLGLRLHSLVSGSTFNLQTRTASAPGG"
     gene            7074..9938
                     /gene="cphA"
                     /locus_tag="VFB37_05640"
     CDS             7074..9938
                     /gene="cphA"
                     /locus_tag="VFB37_05640"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZO21969.1"
                     /translation="MRILDRSVYVGPSLHARFPVIRLELDLGALEQWPTGRLGNSFIE
                     GLVAALPGLAEHGCSYGEPGGFLRRMREDEGTWLGHVLEHVAIELQNIAGEEVTFGKT
                     RSAGPPGVYTVIYEYEQRDEGIAAGELGLRLLCSLLPDSIRPADSVPQGWNWAEARDE
                     FIRFAQRRALGPSTASLVRAAENRGIPWLRLNDQSLVQLGHGKYQQRIQATVTGRTPH
                     IAVELASDKEETNKILATLGLPVPKQECVQTEAQAVRAARRMGFPVVTKPYNGNHGRG
                     ISIGLGTEEEVARGFTVAREHSRSVIVESFLEGDDHRLLVVHGELVAATRRTPGHVVG
                     DGEHTVAELIEIVNQDPRRGVGHEKVLTRLDLDAQAQAMLARANLQADSVPTRGQVVY
                     LRSTANLSTGGTATDVTDVIHPDNREMAERAVRAIGLDVGGVDFLSKDITESYRQIGG
                     GICEVNAAPGFRMHVAPSEGTPRDVAGPVIDMLFPPGAPSRVPIAAITGTNGKTTTAR
                     MLAHIAKMAGHTPGLTTTDGVYIDGQRTVQGDMTGPVSARMVLADPQIDVAVLETARG
                     GLIRAGMGVKEINVGAVLNVQSDHLGLKGIETLEQLAEVKRVVVEVATDCAVLNADDP
                     LVLRMSGHTEAKSICYVTLNPQHTLVREHIRAGGRACALEAGVNGQMITLYDRGSHIP
                     LLWTHLIPATLEGRATHNVQNAMFAAAMAFSLGTKLDAIRQGLRTFDSTFFQVPGRMN
                     IFNEHPFKVLFDYGHNAHAVSVMADLAQRLDVTGRRIVVLAGPGDRRDEDLVAIARAV
                     GGRFDHYICRRDDSLRDRAPDEVPRIQAAALRASGIPEEAISVIPDEQEAIETALRMG
                     QTGDLLLIFADALVRSWKQITKFKPAGHAASGMETTSGMPGLSAQASAASAKGNGHAR
                     PPLLGGEPGQFPEEHPAPSISLEGLIRDERGVHVARETED"
     gene            10175..11836
                     /locus_tag="VFB37_05645"
     CDS             10175..11836
                     /locus_tag="VFB37_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZO21970.1"
                     /translation="MAAPVDQLFVATEINEWALCSALRDRNPFEWSGLEQALAAAALE
                     NADDPEQEIPPALEEGAAFARFERLALQESDPQLRAFLDAAQARHLQHCMDNDMLTLG
                     SGAGSGNFALTQLPSVESLAWAELHNVPTAVVTGSNGKTTTVRLLAHCAQAHGWLPAY
                     CCTEGVLVDGESLAAGDYSGPAGARMVIRERRAQAAILETARGGILRRGIAVSRADVA
                     VVTNISSDHFGEYGIDDLDALAEVKLSVAAVVPAEGLLILNADDAMLRHKAKTLAQRF
                     GRLPPLGWFALDAESPFLQQHRIRGGWTCGVRDGHLRAHYRGTDHDIGPIASMPLTVG
                     GKATYNIANLAGAALAAMVLGVPATTIAAVFGHFGADPADNMGRMMRFEIGGVHVVLD
                     YAHNPAGLRGLLSVAGPLRTHSARLGLVLGHAGNRRDAEIELLAKAAAEFRPDFIVVK
                     ENESHLRGRASGEVPHLIHAALLREGMVESALSMRMTELEAARHALDLAHPGDVLVLP
                     VHAAAARTAVLEMLEAKKSAPPRQNSPGIAAAHESAEITNPDNGG"
     gene            11965..12843
                     /locus_tag="VFB37_05650"
     CDS             11965..12843
                     /locus_tag="VFB37_05650"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZO21971.1"
                     /translation="MSDLVDRFDAEGYAIVRGFLSASEMAEISAETDAMYREGLKHHA
                     SYRDRNLLFEVLNDPKARRRVVLQAHWTAWISPLLERMRRSEKFFAVLEPFLGADIRQ
                     ISHQIHWKPPGAKYTSYRFHQDARFRQGKLTEHDYLRSSVTTGLAIDPQTADNGALRL
                     FPRSHRLGYLGLSDDGPIMVGETQDEELQRAGLDPSAAVICEMQPGDLLLWNLFTVHG
                     SAPNLSSHDRRFMINSYVRASDSDRGEWAFRGGISTPLGTEPEICKYEQLRERPGPFY
                     IEDDWTREAGADREAG"
     gene            complement(12789..14063)
                     /locus_tag="VFB37_05655"
     CDS             complement(12789..14063)
                     /locus_tag="VFB37_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO21972.1"
                     /translation="MTVYAFNSMDRNIITIIGQPMKLALGLTDTELGLVSGTAFAALY
                     AFGGVPIARLAERVNRVHLMAISLAIWSCLTALCGLARNFPQLLMTRVGVGFAEAGCA
                     PPAHSLLSDYFEPGRRASALSVYSCGISFGYILAAVAGGFVAQHWGWRAACAIVGLPG
                     LLLAVLLKIAVREPPREAQIRAPLSLRVELHELAAVGRSLLLSAPILHMVLGVTISAF
                     AAYGFYAFMPAYFSRAFELDYTTIGFIAAMTGGVTVGLGILAGGFIADRLASRSAHWY
                     ALVPALGTLLALPLYLLALLQPRWPGATLLLGSAGFFQYASLGPTFGVVQNAVDPTRR
                     ASATALLYICLSVLALGGGPPFTGWVIDRFTAAGTPLALATRAGLLVTLTFFGWASLH
                     YFLAIRGIARALRANHPASRSAPASLVQSSSM"
     gene            complement(14147..15589)
                     /locus_tag="VFB37_05660"
     CDS             complement(14147..15589)
                     /locus_tag="VFB37_05660"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO21973.1"
                     /translation="MKNMLPITFAFALLSIGALAEPPDPSEQSFRDLYKELVETNTSL
                     SSGSCTLAAERMAAHLRSAGFPDSELHPFATPEHPKEGGLVAIYPGKDPKLKAILLLA
                     HLDVVEAKREDWTRDPFKLVEENGNFYARGSMDDKAEAAIWVDTLVRLKEAHQQPRRT
                     LKLALTCGEETSGAFNGAEWLTQNRRDLIDAQFAINEGASGELDAQGKRIVLDIQAGE
                     KTSRNFRLEVTNPGGHSSRPVKNNAIYHLAGALKKIEAYEFPPQFTDASRAYFSGLAR
                     IEAAKGETDVANAMNALVKNPDDSEAIALVAAKDPTWNATMRTTCVATMLDAGHATNA
                     LPQRARANINCRIFPGTPPEAVRQKLEELVADPEVKVTIVENRSPGAPPPPPPPPAPP
                     LTAAILGPVQKLAAQFWPGVPILPILQPGATDGRFLSAAGIPTYGIEPVFLGPDLGHI
                     HGLNEYVSVKSLLEGRAFLYQLVKIYANQK"
     gene            complement(15610..16914)
                     /locus_tag="VFB37_05665"
     CDS             complement(15610..16914)
                     /locus_tag="VFB37_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO21974.1"
                     /translation="MTENQRDRGLLRAVGPLTFAASIFNGIIGAGIFVVPAALSASMG
                     VYSPLAFVVCGIAIGSVALCLAEGGSRIPTSGGIYGYIEVAFGPLVGYVAGTVLWFGN
                     VLACGGVAAALADLIASLVPASYAPVVHAASIVGLVGGIALVNIAGAAQGARLASIAT
                     VLKLLPLTIFIVAGAFAWHGGGFVVTAPPTPQGFDRGILLALFTLTGMEVTLSASGEV
                     AQPARSIPRALALSISAVTVLYISIQAVAQGILGPALAQSTTPLADAMAQISPALRWM
                     MLVAASVSMLGWLSSDFLGSPRMLFAFARDGLLFSILGRVHKRTHTPYVAILVYAALA
                     IVLALSGSFAELAVLGTLASALLYGSGCAAAYRLARREVAQAGPPLNSRWLGPAAFVG
                     IGSMVAMISLASRTEILGALALVVISALFYLLSVRLATVRPG"
     gene            17011..18351
                     /locus_tag="VFB37_05670"
     CDS             17011..18351
                     /locus_tag="VFB37_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO21975.1"
                     /translation="MLRWLSIPFFLAVAMLADAQSAPPASSAGPAHPILIHTQSLIDG
                     TSAQVRHGQDILIRGNRIVEVSPAGAHAAPADAEIIELGTATVLPGLIDSHTHIFLQG
                     EVPEAGGYDVQLLKFPASYRVARAIVAARRALEQGFTTIRDVETEGAGYGDVGIKRAI
                     NEGYIPGPRMFVATRAISSTGGYSLDDYAPEITVPKGAQIIDGPVEARKAAREQLDHG
                     ADWIKVYMTHRSWVDEQGELVSQPTLTVEELKAIVDEAHGWRRKVACHAYNGIGLQRA
                     LDGGCDSIEHGLEITDAQIAQMIHQGTWYVPTLAVYYYDPDPPDTPSGKRDLKRISDH
                     VISFKKALHAGVKIAFGTDVGGFVWTDPIAQEFVREVELGMTPMQAIQSATSHGAELL
                     GKRGELGVIAPGAIADVIAVPGDPLQDIHLMEKVQFVMKEGVVFRSASGATHTP"
     gene            complement(18389..19537)
                     /locus_tag="VFB37_05675"
     CDS             complement(18389..19537)
                     /locus_tag="VFB37_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21976.1"
                     /translation="MSNHAKRRIRPVLVLALPLVILAAALAASCKYDGQGSITNQHAT
                     LGTKGLWIANGTNVLEYVPNQMIGGTANVGPLVVSSGAFGSPQGVQFDANGNLWVLDS
                     AAMVNGVATAAVLGFSASQVGALATSNAPEPVTVITSTALKLPQQSVFDSQGDQWITD
                     HDNNTILVFTAAQLGQTGLNISPAVIISSTALNGPTGVAFDGAGNLWVANDGDVPQSS
                     GGTSSAGTTIVEFAAAKLPTPPTTGMLTPDLAPDITLNDDGDSSIQGPWALAFDADGN
                     LWSSNAVSATVVEFAKSSLATSGKPTPAAILSSTTVSGNAALDAPHGLCFDDQGNLVA
                     VSSAGPFAVALFAKSQLATGAPTPDTLFIGSATGLSAPQGCVFGPAIM"
     gene            complement(19624..21114)
                     /locus_tag="VFB37_05680"
     CDS             complement(19624..21114)
                     /locus_tag="VFB37_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZO21977.1"
                     /translation="MRLERRDFLRLSGLAALAPGLPLLAQVQAPPVSPATAPRDHPAD
                     MRADYTIRIARSNIELAPDHVISTTTYNGQFPGPLLRFKQGRSVVVDIHNDTETAEQL
                     HWHGQTVPVEVDGAAEEGTPYIAARGMRRIAFTPGPAGLRFYHTHLIAGSNLQGGLYS
                     GQAGPVLIEAAHEPGHYDREVILVLKEFEPALSQGGDMAMHFLEPSRTDPELKRAGES
                     AMNASLRRRMPHGYEVGYKYFSINGRMLGHGEPVRVKAGERVLFHVLNASATEIRSLA
                     LPGHTFTVVALDGNSVPVPARVDALWLGTAERVSAIVEMNRPGVWILGDLADDDRRNG
                     MGIVVEYAGARGTPKWGAPASSRWDYTRFGRGTAHAPDETIPMVFAKVNAARSGFNQW
                     TVNGESFSMKAMSPLFKVHAGRRYRLQMRNASDDIHPVHLHRHSFELVSLAGKPTAGV
                     MKDVVMVGGYQQVEVDFIADNPGLTLFHCHQQLHMDFGFMALIDYV"
     gene            21336..23411
                     /locus_tag="VFB37_05685"
     CDS             21336..23411
                     /locus_tag="VFB37_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZO21978.1"
                     /translation="MSSHLHARREGALGVLLVSCLAVAGLGRAATAPYPDSTLISAMS
                     WDFSSVVTLRKAHGSDLWPLTWAADGNLYGAWGDGGGFDGDNNNIGRVSLGFALLSGT
                     PASGEPDSYGGRNVWGAAPRFAEHRATFGGKVDDLISIDGVLYGQGGLWTAANCGCAD
                     PTLKPGDNRHHRTVTWSADHGRSWEVAAWTSSSGPGTSLQYGRNYQGAKDPAHVYFYF
                     QPDSASDATRIYLRRVLTSQLTADPATPGHFEYLTAIDGEGSGLWSASQTSALPVFED
                     SHVPPGTDANATVVYDEGLGRYLMATQHGNGAGQMGFFEAPAPWGPWRTLGYYEDWGG
                     FNESAGEGNGLSFPSKWMSSDGRTLWAVFSGAGTFDSFNVVKAVLTVGAEIPQIADPA
                     AGTVLAPGERVSATGVGTGLSWSVESLGKTESLVATGTGPSLTFIVPEGAPAGSLIRV
                     TLSNTLTQVSRDYAVSGRSGSLLVGDWMFDEGTGSTTRDSSGEGNTGTLVNGPVWVAG
                     KTGHALDFRGGRAGVRVPGVPTLANLFARGLTVMAWVHPRSDGNSGRILDKDGNSRGW
                     LLKMSEGGVQFVGDEFGVTAVSRKTNRFLSRNAWQHVAATWDGSYKGADIHLYVDGVP
                     ADGTYVNGGGGLRDDSAIPLTLGNRLFDLERGFDGILDDVRIYNRVLTPGEVHEVAMA
                     AVSPSTRAP"
     gene            complement(23432..24130)
                     /locus_tag="VFB37_05690"
     CDS             complement(23432..24130)
                     /locus_tag="VFB37_05690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZO21979.1"
                     /translation="MNRSTTTVIFDVEGTLVDCVPQTLESWRQSLAESGHSFRRDELQ
                     PYSGMDGEEMLDGLLPALSKEGRQSIAKRQGELYRDQHLRAVRPFEGVRRVFVALKRA
                     GLALAIATTCTRDELAVYDKQMRVLALTDVVTCGDDVPHGKPHPDLFRAVLKKLRVTE
                     PSEVLTLGDTPYDARAASALGLEPVGVLTGGFSRDALLFAGCSCVLDGVRALVPHVAS
                     TFLTTSPALQSASK"
     gene            24503..24985
                     /locus_tag="VFB37_05695"
     CDS             24503..24985
                     /locus_tag="VFB37_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZO21980.1"
                     /translation="MSKSLTDQSFVTQAAQGGMAEIDLSNLALQKSQDSQIRSFAQRM
                     VQDHGKANSELKPIAQKLGLQVPSDTDAKHKQEMQKLGEESGAKFDAAYSKAMEKDHT
                     KAVELFTKASKSDTLKPDLRNFAAKTLPVLETHHQMADNLPKSSMRSAQSSNSQPRSQ
                     "
     gene            complement(25139..27085)
                     /locus_tag="VFB37_05700"
     CDS             complement(25139..27085)
                     /locus_tag="VFB37_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO21981.1"
                     /translation="MINFTDVTLRRGTRVLFSGATFGIFRGEKVGITGENGSGKSSLL
                     GLVRGELQPDAGTFELPSNLAIAHVVQELLPLETAAIEFVLDGDAALRAVERSITAAQ
                     ARDDGIRLGELYAQYAAAGGYEARSRAGRLLHGLGFGSADELRPVHAFSGGWRVRLNL
                     AQALMCPSDLLLLDEPTNHLDLDAILWLESWLLDYPGTLLLIAHDREFLDRVVQRVVN
                     LEHGHARAYRGNYSAFEEQRAAELAEQSTLYARQQREIKHMESFIERFRAKASKARQA
                     QSRLKALERMQRIAPAHVDSPFEFSFAQPDKLPRPLLTLEGQSVGYDGHAVLEEVNMT
                     LAPGDRVALLGRNGAGKSTCMKLLAGELTGLSGLRTEARELRIGYFAQHQLEQLRGED
                     TILGSLRRTGMERAAAATEQELREFLAGFGFRGERVFEPVAPFSGGEKARLVLALITY
                     QRPSLLLLDEPTNHLDLEMRQALAVALQDFAGAVVLVSHDRHLLRTVADRFQIVHGGR
                     VTPFEGDLEDYAKWLMERSVGSMADAAQEPASEAISGSAAARQRRRQEAERRNRLTPL
                     KARVAQCDTELARLTAEASALQAALAEPGLYANEEKDRLRDLLARQATIRRETEQMES
                     AWLEASELLEAQMQSTGDEGARSG"
     gene            27133..27840
                     /locus_tag="VFB37_05705"
     CDS             27133..27840
                     /locus_tag="VFB37_05705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO21982.1"
                     /translation="MTSPTRKPRVIDLTQPLGPATVMWPGSSPPSFRTVDDYREAGCF
                     SRQVSLHEHTGTHLDAPAHFIEGAQTVDQIPVEQLVCPVAVIDIEPHAARDPDYALSI
                     ADIEQNEARHGEIPRGSAVMVHSGWAKHVANARAYLGERGDGGLRFPGVRSAAALWLI
                     RHREITGLGIDTPGIDPGEDTEFTVHRHATLPNGLWQLEGLVNLDQLPARGAMVFVGV
                     LPFVGGSGAPARVIALV"
     gene            27923..30634
                     /locus_tag="VFB37_05710"
     CDS             27923..30634
                     /locus_tag="VFB37_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO21983.1"
                     /translation="MSGLTEEQWRLFSDTLGEALEMPESERASWLAGVAGTSPEVAAA
                     VQRALAARDRSGFANFLSGPLLDSSPPAEVTLVGRRVGPYVIESEIGRGGMGSVWRAR
                     RTDGRFEGTVAIKFVHPYWIGHTGEERFRAEGLMLGRLDHPNIARLIDAGVIDGMQPY
                     LVLEYVEGEPIDIYCNRLQLGVQARVMLFLGVLAAVAHAHSHLIIHRDLKPANIFVTR
                     HGSVKLLDFGIAKLLSEEPARTPTQTLGQALTPQFAAPEQLLGPTVTTATDVYALGVV
                     LFVLLTGELPIQVSSRSQAELIHAVLTEEAPRASTITSLTLPHRRALGGDLDNILGKA
                     LRKNPEERYASVGALAEDLRRFLSDEPVSARPDTVPYRVAKFVRRNRGSVASGLLIAI
                     GLIGTSVFALTQMSAARARRDVALEEAKRANAQADLTQYLLDDRLSRLSPEAERQRLE
                     RARRFVAARFRADPLHAAPLLIDVSGRYIDIGDFRTAAQVIVEVEAIGRQFDDAAILG
                     QLACLRTQDLAIARDFTSARLQLTTGLAQMQRLHPVPLGLEAECATAEAFVVQQDGDF
                     ARAVNRLRSTVADLDKGGMHGAGRYTAATNDLTRALSMAGQYHDAWEVGSRNVTLASD
                     LGRADTGGYFAYVANACSALVNGGQPIRALAYLQAHTDSVAHEADFSGMSTGIRGCWA
                     LAQLQMGHAAAADPTLVEIADKAEQSGISYQASLYHALAVKSALARGDLATAQLRWQS
                     LASDEEHRLATGERGIEVVRLLLVQARVELAQGRPQEALHSLDRAAGLVAERHQPTNP
                     DSRELEELRSRALLAQRHYGEAARQAQSAIQLAQTSAVDAKSSAYVGEALLLLARAQA
                     ASRDQRASISAQSALPHLLTNLDPTHPLIAEARQLEAGSPAPAGSPE"
     gene            30639..30962
                     /locus_tag="VFB37_05715"
     CDS             30639..30962
                     /locus_tag="VFB37_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21984.1"
                     /translation="MTGEPLMSRSRGSSPWRWWVLALAAVAVSSSYYESDVIGPIADL
                     LHRQRGFTQSQLGMLNGIISIPNVALAFFSGLLIDRFGAARVALWTAAVGMLGASQRS
                     ALPTH"
     gene            31062..31286
                     /locus_tag="VFB37_05720"
     CDS             31062..31286
                     /locus_tag="VFB37_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21985.1"
                     /translation="MAAANLVAGGLADRAGAGPANAGGYAPMMGFFFVLSLVALSSAV
                     LLWRRERGPHGHGLEDASARSRELYEIEPG"
     gene            complement(31345..31674)
                     /locus_tag="VFB37_05725"
     CDS             complement(31345..31674)
                     /locus_tag="VFB37_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZO21986.1"
                     /translation="MAKIYVGNLPFSASEADVRALFSQHGTVESVSLPTDRETGRPRG
                     FGFVEMSQADAARAIQSLNGHDMGGRQLRVNEAQDKPRSGGGGRPGGGGGFRGGNSGG
                     GGGRDRW"
     gene            32282..34654
                     /locus_tag="VFB37_05730"
     CDS             32282..34654
                     /locus_tag="VFB37_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZO21987.1"
                     /translation="MNDSAGMESIAFGAPGIAPTWCSSDKDFVTTALGASRLWVTIGH
                     GIINEIYWPSTGEPQVRDFGFYLVGANRWLDLKRLQRYHLVTPGPFLPLLSITHYGVD
                     YRLVLEILPDPRRDVLLIRFELDGPYRLVVILAPHLGSTGRDNSAWVEHSVGYACRGD
                     AAVCLAADAPLLHMSAGYVGSSDGWQDLERHTKLTYSFTHAPQGTVALTAEARGRSGV
                     LALGFSESARGAHTLGRTALAMGFDALRAEFLQAWRTWGSQLQLPSPDATLAHAGLLS
                     ATVLKIHEDRSYPGAVVASLSVPWGNSTDALGGYHLVWPRDATLTAFALLAANQYNDA
                     RHILAHLAASQSADGRWPQNYFPSGTPYWTGVQLDETSFPVLLAAKLRELGEEDLPGT
                     AHMIRAAVGFIVRTGPASDQDRWEENPGVSPFTLAVAISALVAAAPWLTDEEREFADA
                     LADDWNERLESWCYVRDTPLARELGVTGYYVRIASIDKSGELAAQVRLRNRAGEMIRA
                     CALVSMDFSYLVRLGLRSARDPRIQDTLVVVDRVLRVQTPSGGIYHRYNEDGYGEYPD
                     GRAFDGSGVGRAWPLLVGERGHLAMQAGEDPLPYLHTMWNCASAGGLLPEQVWDSDPI
                     PHLELIPGRPSGSAMPLLWAHAEFLKLLVARERGRPIEMLQSVEQRYAGSQSRSAQDW
                     RWREEVPLARLEAGRGLLIEDREPFTAHLGFDDWQGLEDRPAVARPFGIWSVHLMADE
                     LANHKVLNFTRRHADGWEGVNHEVWLDHPPTEHALEHAGSHALPGSPPES"
     gene            complement(34560..36833)
                     /locus_tag="VFB37_05735"
     CDS             complement(34560..36833)
                     /locus_tag="VFB37_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZO21988.1"
                     /translation="MAHGASVAVFLAQIITLLVCGRFFGELMQRIGQPPVMGQLIGGI
                     LLGPSVLGMISPALWHSLFPPGAAQHAMLDAIAQLGILLLLLVTGMETDLSVFRDARR
                     TAISISLTGILVPFICGVILGSLVPDSILPANGQRLITTLFLGTALSISSVKIVALVV
                     RELGFLRRTVGQVIVAAAIIDDTIGWIIMSVIFGLALHGSIDLATLSRSVLGTALFLV
                     VSFTVGRRLVFMLIRWANDHIQSELPVIAVILAVTGLMALLTSAIGVHLVLGAFVAGI
                     LIGQSPILTRHIGEQLRGLIIALFMPVFFGLAGLTTDVSTLARSDMLLLTAGMIVLAS
                     IGKFTGAFLGGRIGGLTYTESLAVGCGMNARGSTEVIVASVGLSIGALNQSLFTAIVA
                     MAIITTMSMPPLLKWALQRIPMSPEERARLEREEFEAQGFVKHIERLLVAVDDSPSGK
                     LASRLVGLLAGVRGIPTTVLHFDNESAERSADNRAHVKQSAAAIKADAVAADESANPK
                     SPATVEITTRAGESAASAETIAAEARKGYGLLFIGREPVAQTNTFEPQITRSAVEFAG
                     AFAVTISRHGRRRNPLSDPLRILVPVSGTSLSRDAAEVAIALAQGSHGAVKALFVANP
                     VRHVRAWRQRFGNVLAPENTGGAAIREIVDIGEHYGINVHGVIRRARTTRDAILREIE
                     RGGHTLLVMGVSPRSGEQLFFGELAAEVLEHSPCSIVFLSSEAPITTPEESREERVNL
                     HAREHARSADDRATPRD"
     gene            complement(36904..40794)
                     /locus_tag="VFB37_05740"
     CDS             complement(36904..40794)
                     /locus_tag="VFB37_05740"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21989.1"
                     /translation="MNEVSAGEATPQVHLLSNGRYHLMLTGAGSGYSRWRDLGVTRWR
                     EDTTRDDWGAYFFLRDLHNGAVWSATEQPCGSAAGGGTVTFSESRAEISRPDHGLSTT
                     LEILVSPEHDAEARRLSITNSGDRSRELEITSYAELMLAPPAADAGHPAFLKLFVQTE
                     RVRYPDAILATRRNRSPDEPHIWAAHHATVEGALLSEPEVETDRARFLGRGHEVGEAS
                     AIVDGRPLSNTVGTVIDAVFALRYRIRVGAGETARLCYWTSVAASRDEVLNLVKRQRE
                     AGSFARADEGARKQAQRDLQERQLAPETAALFRRLAGHLLYANGAMRAPPEVIRAGSG
                     PPGALWAQGISGDLPLLLLQLDAIEQLPMLRQFLQAHEYWRARGLGVDLVILSTQSSP
                     GTQLPAAVKAEMEGRPKHDPAEGTGIRGSVISLGADKIPAQTCALLRSAARVVIDPGR
                     GSLAEQLGRASARAPSGVPRAQDGGLSQRRPPAQRAQPPQQSDLEFFNGLGGFAADGR
                     EYVTILAPGEATPAPWVNVVTNPHFGFQVSADGGGFTWSRNSREHTLTPWSNDPVVDR
                     PGEVLYLRDEESGELWSATASPAGHAAATYTARHGQGYSRFEHSVEDLAVDLLMFVSM
                     ADPVKISRLRIRNDSQRARKLSVTAYAEWVLGSSRGAAAPFILTDLDSDTGALFARNP
                     WDGTFGSRVAFADLAGRQLRWSADRSEFLGRHGTLRRPAALAGRAELSGRVGAGLDPC
                     CALQTSCLLRPGETVETVFFLGDAEDASAARTLLTRVRKTDLESLFQQVVSQWDELLG
                     TVQVKTPDRALDIILNRWMLYQAIACRIWARTAFYQASGAYGFRDQLQDSMAVIVTRP
                     DLTREHLLRAAGRQFPEGDLQHWWLVPLGQGVRTRIADDTVWLAYCAHHYIETTGDVA
                     VLDEPIPFLSGPTLKAGQHDAFFQPERSAQSASLFEHCARALDHSLKVGEHGLPLFGT
                     GDWNDGMNRVGEAGRGESVWLGWFLQYALTSFAPLAAARGETARAQKWRAHAAAVQKA
                     LELAWDGEWYRRGYFDDGTPLGSATSPECRIDSIAQSWSVMSGAADPARARRAMASVE
                     EQLVRPDPGLVLLFTPPFDRIPLDPGYIKGYPPGIRENGGQYTHAATWSVVAQAMLGD
                     GGKAAHWFSLLNPIHHARSPADVETYKVEPYVVAADIYSVPPHEGRGGWTWYTGSAGW
                     MYRAGLEWILGLRLQAGRLLLQPCIPPTWRGFEMSYRHGSTLYRIAVENPKGVSRGIS
                     RVELDGQAVAHAADGLALADDGTPHRIRVILGSG"
     gene            40932..41642
                     /locus_tag="VFB37_05745"
     CDS             40932..41642
                     /locus_tag="VFB37_05745"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZO21990.1"
                     /translation="MGKKEHRLAEIDWQRIGTELDSCGAARIDGLLGASECRALAALY
                     ADERHFRSRVVMSRHGFGRGEYQYFRYPLPPLIAELRAQLYPSLAPIANRWNEKMAIG
                     SRYPATLEPFLARCHKAGQPRPTPLLLRYQPGDYNCLHQDLYGEHCFPLQLTILLSEP
                     GKDFTGAEFVLTEQRPRMQSRVEVIALQRGDAVIFAVNQRPVQGVRGIYRVKHRHGVS
                     RVRSGERYSAGVIFHDAK"
     gene            complement(41660..43393)
                     /locus_tag="VFB37_05750"
     CDS             complement(41660..43393)
                     /locus_tag="VFB37_05750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO21991.1"
                     /translation="MSSLRPGRASQRGARQAQVFVLCLAFSSALAAATLDVRVLATHS
                     GDPPAAQVVRGSLDSGFTSAGYAELAARGTPFWLRLQPAAAIDGGSVPVLTVHTGRLT
                     QVRVYAAASSRTDGTGDGQDVGSELPRAARLPGFGGAQEVVFVLPAAAASGRQALYAR
                     IVPAVRGSELPRISLASLEEVLRAGTTRARMIALAFGALMAMSIAALLIWLVLGEKLF
                     ILYAALFSLQALYVVYLSGQGFEWPLLSFALPLSSHAWNVPVALGGAAACLFTRELAD
                     LRRFSPRVYTIFGWLAWAFVALTFANLLKLIGWGLIVNAAGNALFLASATFTLIVLFV
                     AWRRGSRAAGWFFLAWALLEAFTIATTATLLLKGDDDGRLLYVGLPLSMVAAALLVAL
                     GLADRLREQRLALSEAERRAQTDSLTGVLNRRSFLERLDAACLRARARGLPISLLFID
                     LDHFKQINDSRGHAAGDACLTSIIAPIQAELRQSDVIGRYGGEEFVVILSSADAAAAY
                     PIAERILARVAGVRVEGFGEPISLTCSIGVATSDSPGAWGENLIARADAAVYVAKRSG
                     RNRVEVALLAA"
     gene            complement(43466..43804)
                     /locus_tag="VFB37_05755"
     CDS             complement(43466..43804)
                     /locus_tag="VFB37_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21992.1"
                     /translation="MRQAGFRVAKLVAIFVLGISLGGCIVAAGPPNEYYVGGPVDVAP
                     PPPRVEEYGAPPYPGYIWLGGYWNWVGGRHEWVSGHWERPHPHERWVSHHWVHQRDGW
                     HLARGHWERR"
     gene            43905..44585
                     /locus_tag="VFB37_05760"
     CDS             43905..44585
                     /locus_tag="VFB37_05760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZO21993.1"
                     /translation="MPDAREDIVFLFDVDNTLLDNDAVQADLGDHLESEFGRAARERY
                     WALFETLRSELGYADYLGALQRYREEDPDDMQLLRMSAFLIDYPFASRLYPDALSTLA
                     HCGRSGPVALLSDGDVVFQPRKVERSGLWAAVEDRVMIYLHKEQMLDSVERRYPARHY
                     VMVDDKLRILTAMKKIWGTKLTTVFVRQGHYALDIANISIYPPADVTLECIADLRAHT
                     LAELSGAT"
     gene            44582..>45999
                     /locus_tag="VFB37_05765"
     CDS             44582..>45999
                     /locus_tag="VFB37_05765"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HZO21994.1"
                     /translation="MSSPQVNPLAGKPATPDMLTDVPRLVTAYFALQPDVAIPAQRVA
                     FGTSGHRGSALERGFNEWHVLAISQGICEYRRGRGIDGPLYLGIDTHALSVPASVSAL
                     EVLAANGVEVMLSERDEYTPTPALSHAILTYNHGRQSGLADGIVVTPSHNPPPDGGFK
                     YNPPNGGPADKDVTGWIETRANELLGAGLQGVKRIGYEQALRAATTHRYDFLNRYVDD
                     LAQVIDLDPIRASQVNVGVDPLGGAGVHYWAAIADRYRLKLNVLNTAVDPTFRFMTVD
                     WDGRIRMDPSSPYAMQSLIHLKDRYDIAFACDTDHDRHGIVTRSAGLLPPNHYLAVSI
                     DYLFRNRPRWPAAAGVGKTVVSSGMIDRVTSRLGRRLYEVPVGFKWFVSGLSDGSLGF
                     GGEESAGASFICLASGPWSTDKDGIIPCLLAAEMTARKGRDPGDLYRNLTREFGEPAF
                     ERVDAPATREQKQILKRLSPAQV"
BASE COUNT         8212 a        14873 c        15062 g         7852 t
ORIGIN      
        1 tcttcatcta tcagggattc aacaccaggg tgggctggat gcacacctcg agcggcgtgg
       61 acaacgtcga tgagttcgcg gaaaccatcg tcaccgcgcc ggatggctcg cgagcctatc
      121 ggtatggcaa ggagctgcga cccctcacca gcaagcagat ctcactgtcc tatcgggccg
      181 cgaacggctc gatggcgacc cgtacgttca ccacctacgc cacgcaccac ggtcccatcg
      241 tgcgtgagga aggcggcaag tggatcgctt tcgcactgat gaaccggccg atcgcggccc
      301 tcgagcagtc cttcctgcgc accaaggcaa aggattacgc cagctatctc aaagtcgcgc
      361 agctgcgcgc caactcctcc aacaacacca tcttcgccga ctccaagggc gagatcgcct
      421 atctgcaccc gcagttcgtc cccgtgcgcg acgatcggtt cgattaccgc gcgccggtgg
      481 acggcagcga cccggcaacc gactggaaag ggctgcacag cctcgagagc ttgccgcacg
      541 tggtgagtcc cgccaatggc tgggtcatga atacgaatga cgcaccctgg acggctgcgg
      601 gtgccgacag cccgaaggct gcggactatc cgcgctacat ggatgaggcg ggcgaggacc
      661 cccgtgggcc acacgcgatc cgggtgctga ctgcgcggca ggacttcaca ccgcaaacgc
      721 tgattgcggc ggcattcgat ccctatttga ccgagtttgc gcaactcatt ccattactcc
      781 tcaaagcctt cgacgcgttg ccacaaagcg atcccggcag aacgactctg gccggcccga
      841 tcaagctgct gcgcgactgg gactatcggt ggggcctgac ctcgcaaccc acgtccctgg
      901 cggtgttctg gggcgatgcg ctctggcacg tctgcgctga gccggcgaag gcggcacgtc
      961 tttcggtctg ggactacatg gccgagcaca cgagcgatgc gcagaaactg gccgcgctca
     1021 cagaggcgag ccagaggctc acccatgact tcggcagcta cgccgtgcct tggggcgaga
     1081 tcaatcgatt tcagcgcaac gatggggcca tcgtgcagac cttcagtgac gtgaaggcga
     1141 gcattccggt gcccttcacc tcctcgaggt ggggttccct ggcctccttc gctgcgaagc
     1201 gctatcccgg gaccaagtgt tactacggca ccgaaggcaa cagcttcgtg gcggtcgtgg
     1261 agttcggtcc acgcgtgcgc gcctggggaa tcacagccgg cggtgaaagc ggcgatcccc
     1321 ggtccgttca cttcctcgac gaggcgcaac gctatgccga cggcaacctg cgcccgatct
     1381 acttctatcc tggcgatctc aaagggcaca ccgaaaagcg ctaccacccg ggggagtagc
     1441 ctgaggccta cggcgtcccc tgcaggtact gattgaacca atcgaccagg cgggtcatga
     1501 catcgatctg gtcggcccgc ttgaagaaga agtgcccctc gtctggatag acgacgaact
     1561 cggttttgac tcccaggcgc ttcagggcgt tccaatattc atacgactgc gtgatcggca
     1621 cctcggcgtc gcggtcgccg ccgaccacca gtgtcggggt cttgaccttc ttgatgaacg
     1681 tgatcggcga gctgcgttcg tagactttcg gatcatcgta aacggaagcg ccaaagtagg
     1741 ggatcatcca ggtgtcgatg ttgttggtgc cgtagtaact gagccagtcc gagaggccgg
     1801 ctccggagac ggctgccttg aaacgatccg tctgcgtcag cgcccacatg gtcatgtaac
     1861 cgccgtagct ccagccggcg atgccgacgc gggccgcatc gatcggatcg ctcttctcca
     1921 ccgtgtccag gcccctgagg atgtcgcgca gatcgccata gccgaaatcc ttcacgttgg
     1981 cctgtgtgaa cgcctcaccc tggccgtagc tgccgcgcgg attgggcttg aagacatagt
     2041 agccctggct ggagagtacg gcatcgaagg agtcggggct ggccggaaag agaggatagt
     2101 gtgcgaaagc cgggccgcca tgcacctcga ccaccagcgg ataatgcttg cccgccccgc
     2161 cgggaaccgc cggctcgatc agcattccct gcacgtcgaa gcgatcgctc ttccaggtca
     2221 cgatcttcgc gttgccggtg atggaatgca ccgccgcatt gaggcgcgtg atcggtttcc
     2281 acttgcccgg tgtgccggtc tcgacctccg gcgccatcgt gtaggactgc cgcacgatcg
     2341 cggacatggc cgcgtcccgc gaaagagaaa taccggcatc acccggggtg aagccgagca
     2401 gactgttggc ccagatcatc tgcgggcccc gccagatctc gcgctgcgac ttcgctttca
     2461 cgtcgatcga ggccagcact tcatctccgg cggaaaattc cgtcgcgacg atgtgggacg
     2521 aggatccgtt ccaggtgatc gtttgcaccg aggcgcgcag atccggtgtg aggtccacgg
     2581 gcgtgccgcc acttgccggg accagataga cgtctcctcc ggtgatgcct tcgtcactca
     2641 tgatgccgct gatgtaggcg atctgcgcgc catcgctcga ccaccggggc gaggcgatct
     2701 gcagtttcgg cttgagcagt gaggtggtgc tgccggtttg cgtatcgatc agctgcagct
     2761 cggcgatcca ccagttgtca tctcccgagc cgggtgcggc gctgaccaca aagcgcttgc
     2821 tatcgggtgc ccagtcgtac tcgtagatgt tcaggttgcc ggggccgagc aggtggatct
     2881 cgcctccggt tgcggatatc agcgcaaggc gctgctcata gatcgtctcc gacaggacgc
     2941 cggcatcgcg cgccagcggg ttgagcgggc ccggcgtgcg tggcgcgccc tccgaataga
     3001 gaaacgcgat ctgcttcccg tctggcgacc agcggggcgt gtcgagcggt ccatgcgcgt
     3061 tcgtgaggac cttcgcatcg ctccccgtct cgctggcgat ggcgagctgc gactggccgt
     3121 tggagtcagt cgtgacgaag gcgacctgcc tgccatccgg tgaccaggcg gcgctgtgct
     3181 catcgcactc catgctcgga caggctgtca gccgcaccgc ggcggagccg ttgcgagcat
     3241 tcgccatcca cagcatagag gaggcgacat cggctccgcc gcgtttcgcc gtcacgacgc
     3301 tgccatagat gacgcgcttc ccgtccggag agagtgcaac ctctgcgatc gggtgcacgg
     3361 acgtgagcgc ctctaccgct ttggcgatcg agggcgactc ggcgccggcg atctccgaca
     3421 gcacgcagca gagtcctgcc gcagccagcc gcgccagacc cattgctcgc tcgttcatca
     3481 acttcccctc ggccagagtc ttgccggcca tatctcacca gagcataccg gaagcgccgc
     3541 ggccaagccc agccgcagga tgcaggtaca ctgcaacagt tttcgcagcc gagagtaacg
     3601 aggagcgagg atggtgacgg cggaatgcat gcgagcagcc cggcgaggtg cgggtgcgtg
     3661 gccaacagtt gccttgggac tgctccccgc cgctgcggcg gcagccggtg gccacgcaga
     3721 actgttcgtc aacggcaata tctacaccgc ggatgagcac aattcgcacg ctcaggccct
     3781 cgtgtccgag gccggccgca ttatttacgt gggcaccaac agcggcgcgc gccagcacgc
     3841 accgaaagac tccgagaccc tcgaccttca tgggctgacc gtttttccgg ggctcaccga
     3901 ctcccacgct catctgcggg acatcggttt ccgcgagctc agcttcaacc tcgaaggcac
     3961 gcgcagcctc gccgagctgc agaggcgctt gcgtgagcgc gcacgcacgg tcaagggcgg
     4021 agcctggatc acgggcggcg gctggatcga gtcgcgctgg agtcccccgg cgtttccaac
     4081 tcgcgccgac ctggatcagg tggtcaacga tcgtccggtg gtcctcgagc gcgccgacgg
     4141 acacgccatc gtggtcaaca gcatcgctct ggagcgggcc ggcatcgatc gcaacacccc
     4201 cgacccaccg ggcggccgga ttctcaagga gccgggtggt gagccgaacg ggatgctgat
     4261 cgacaatgcg atggagctcg tccagcacct cgttcctgcc ccaaccgatg agcagacatc
     4321 gaaggcgctg caggcgggcg cggagcgcag cgtgcggctg ggctggacgc agctgcagat
     4381 tgccggcaat tcctttcacg aggtcgatct gatctgccag ctctatgcgc agcacctcat
     4441 caagctgcgt ctttatgatg caatctatgg gccgagcgat gatgctcaga ggctgttgcg
     4501 ggaaggccca tcgatcgatg gttgtggcgg acggattacc gtacgcggca tcaagctgta
     4561 tatcgatggc gcgctgggct cgcggggtgc agcgttactc gagccttaca cggacgcacc
     4621 tgacagccag ggtctgctgg tcaatcagcc ctcggccttg cagccgattc tcatcgcagc
     4681 gctcaagcgg ggtatccaga tcgaaacgca cgccatcggc gatcgcggca atcgcatcat
     4741 gctggatctg taccagaagg cttttgcgac ggtgcctgtc gcgcagcgcg caatcgctca
     4801 accgcgctgg cgaatcgagc acgcccagat cctgagcccg gccgacattc cgcgcttcgc
     4861 tcagctgggc gtgattgcat cgatgcagcc ctcacatgcc atcagtgatt tcttcttcgc
     4921 gcccaagcgc ctggggaccg agcggctcgt gggcgcctac gcctggcgca gccttctgga
     4981 ctccggagcg accgtcgtgg cgggaacgga tgcgccggtc gagaaaggtg atccgatcat
     5041 cgaattctat gcggcggttt cgcggcgctc gctggaagga ttcgccgacg agaactggca
     5101 cctggagcag cgtgtctcgc gagcgcaggc gctcaggatg ctctcgatcg ggccggctta
     5161 cgccgcgttc caggaaaagg agcgcggcag catcgaggtt ggaaagatga ccgacttgag
     5221 tatcttctcg gctgatctga tgcagattcc ggagcgcgag atcctcaagg ctcacctggt
     5281 catgacggtc atcggcggcg agaaagtgta cgaggctcag cctgcgaaag agtgaccccc
     5341 ggaagagtga gcgccggatc gcctccgcga ggagtacgac ccggcgtcat ccatttgaaa
     5401 aaactgcctg cggtgctgat tccgggctct tcagccgccg gcccgcttgc cgttcttgtc
     5461 gtcgctgagc tgctggaccg ccgcgagggc tttgtccacg ttctccgccg gggccatgcc
     5521 gccaatggtg gctgcgatgg tgccgtccgg cttcacgatg aaggtcgtgc gctcggcaaa
     5581 tccgtggtcg atctcgaccc cgcgggtatc tttcagtccc ggcttgcctt ccatcacctt
     5641 cagctcgaac gacttggcga tcttgccgtc ggcatccgag gcggtcggaa acttgcccgc
     5701 gcagtagttc gggtcggccg agaagtcgtt caggcgcttg atgctgtcca gggacacgcc
     5761 gatgatgctg gcgcccgccg ctttgaactt gtcgctgttg acggcgaagg tgtgtgcctg
     5821 gatgttgcat ccattcgtgt aggcggaggg gtagaagtag accaccacgg gccctttctt
     5881 cagggcatcc ttcagggagt agtggaacgc cttaccggca aaggaagcct gggctttgaa
     5941 ctcgggcgcc tggtcgccct ccttcagggc agccagcacc ggcaggctga tgacactggc
     6001 gagcacacaa gcaagtagcc gttttctcat agatcacctg gtgggggact gcgaacgcct
     6061 atgatggcac gcgtccggag ccgcccgcga gagcaaaggg ccggccggga acggtgggac
     6121 gcgacagccg gcccgcggcg agtcatcatt ggcgtgcggc gccctgcggg gcggcggatc
     6181 cccgccggcc cacaaatctt cacaaactga gcagcagtca gggcgcctgg aggagcacga
     6241 caggatgcct agcagagttc cggagggcgc gaagcgcggc tggatcgttc cgatcggcgg
     6301 ggccgagaac aaggaaaacg atcgccgcat tctcgagcgt ttcgtgcagc tttcgggcgg
     6361 ggagagcgcc gacatcgtgg tgattcccac cgccagccag ctgtacgaga cgggtgcccg
     6421 ctatgagcag atcttcagcg agatcggcgc ttcccgggtg agcgtcatgg acttcgacac
     6481 gcgccgggat tgccaggagc ggggccgcct gcagcgcctg gaggaagcca gcggcatctt
     6541 tttcacgggc ggcaaccagc tgcgcgtcac gacccttctc ggcggtaccc cggtcgccaa
     6601 gctgatccgc gcccgtaacg cgaacggggt gacggtaggc gggacgagcg cgggcgccag
     6661 tatcctgagc gagcacatga tcgcctccgg ggacgaggga tcagccgcga tcgccgggag
     6721 cgtgcggctc gcaccgggcc tggggctgac caaccgcttc atcatcgatc agcatttccg
     6781 ccagcgagac cggctgggcc gcctgatcac ggcactcgcc tacaacccct ttgcggtggg
     6841 catcggtctc gatgaggaca ccgccgccct gatcggcccg gacgatgtgc tcgaagtcga
     6901 aggcagtggc agcgttacgg tcgtcgatgg agccgacgtc acctactcct ccatccacga
     6961 agtcagcgac ggtcagccga tctgcatgct cggccttcgc ttgcactctc tggtttccgg
     7021 ctcgaccttc aacctccaga cccgcacggc atcggcccca gggggatagc cccatgcgca
     7081 tcctcgatcg ttcggtctac gttggcccat ccctgcacgc gcgctttccg gtcatccggc
     7141 tcgagctcga tctgggagct ctggagcagt ggcccacggg ccgcctcggc aactcgttca
     7201 tcgaggggct cgtcgcagca cttcccggtc tggccgaaca cggctgctcg tacggcgagc
     7261 ccggcgggtt tctgcgccgc atgcgcgagg atgaggggac ctggctcggt cacgttctcg
     7321 agcatgtggc catcgagctg cagaacattg ccggcgagga agtgacgttc ggcaaaactc
     7381 gcagtgcagg accgcccggg gtctatacgg tcatctacga gtacgagcaa agagatgagg
     7441 gcatcgcggc cggcgagctc gggctgcgcc tgctgtgctc tctgctgccc gactcgatcc
     7501 ggcctgctga cagcgtcccg cagggctgga actgggctga ggcccgggac gagttcattc
     7561 gatttgcgca gcgccgggcg ctcggtcctt cgaccgcatc gctggtgcgc gccgccgaga
     7621 atcgcggcat tccgtggctg cgcttgaacg atcagtcact cgtccagttg ggtcatggaa
     7681 agtaccagca gcgcatccag gccacggtca ccggtcgcac gccgcacatt gccgtcgagc
     7741 tcgccagtga caaggaggaa accaacaaga tcctcgccac gctggggctg cctgttccga
     7801 agcaggaatg cgtccagacc gaggcccagg cggtgcgcgc ggcgcgtcgg atgggtttcc
     7861 cggtggtgac caaaccctac aatgggaatc atggccgcgg catctcgatc ggactcggca
     7921 cggaagagga agtcgcacgg ggatttacgg tcgcacgcga gcattcgcgc tcggtcatcg
     7981 tcgagagctt tctggaggga gacgatcacc ggttgctcgt cgttcacggc gagctggtgg
     8041 cggcgacgcg ccgtactccg ggacacgtcg tgggagatgg cgagcacacg gtcgccgagc
     8101 tgatcgagat cgtgaaccag gatccgcgcc gtggcgtcgg ccacgaaaag gtcctcaccc
     8161 gactggacct ggatgcgcaa gcgcaagcca tgctggcgcg cgccaacctc caggcggact
     8221 ccgtgccaac ccgaggtcag gtcgtctatc tgcgctctac ggccaacctc tccaccggcg
     8281 gcacggcgac ggacgtaacc gacgtcatcc atccggacaa ccgcgagatg gccgagcggg
     8341 ccgtacgcgc aatcggactg gatgtggggg gtgtggattt tctgtccaag gacatcaccg
     8401 agagctatcg ccagatcggc ggcggcatct gcgaggtcaa cgcggcgccc ggctttcgca
     8461 tgcatgtggc cccgagcgaa ggaacgccgc gggatgtcgc cggcccggtc atcgatatgt
     8521 tgtttccgcc gggcgctccc tctcgcgtgc ccatcgcggc gatcaccgga acgaatggca
     8581 agaccacgac tgcgcgcatg ctggcccaca tcgccaagat ggctggccac accccggggc
     8641 tgacgaccac cgatggagtc tacatcgacg gccagcggac ggttcagggc gacatgacgg
     8701 gcccggtgtc ggcgcggatg gtgctggccg atccgcagat cgacgtcgcg gtgctggaaa
     8761 cggcacgcgg cgggctcatc agggctggaa tgggcgtgaa ggagatcaat gtcggggccg
     8821 tgctcaacgt gcagtcggat catctgggtc tgaaaggcat cgagacattg gagcaactgg
     8881 ccgaggtgaa gcgtgtggtg gtggaagtag cgaccgattg cgcggtgttg aacgccgacg
     8941 atcctctggt gctgcggatg tcagggcaca ccgaggccaa gagcatctgc tacgttaccc
     9001 tgaatccgca gcacaccctc gtcagagagc atattcgtgc cggcgggcgg gcctgtgcgc
     9061 tcgaggccgg cgtgaacggc cagatgatca cgctctatga ccgcggcagc cacattccgt
     9121 tgctgtggac gcatctcatc ccggccacac tcgagggcag ggcaacgcac aacgtccaga
     9181 atgcgatgtt tgcggcggcc atggccttct cgctcggcac gaagctcgat gccatccggc
     9241 agggtctgcg caccttcgac tcgaccttct tccaggttcc gggtcgcatg aacatcttca
     9301 atgagcatcc gttcaaggtg ctgttcgact atggccataa cgcccatgcc gtctcggtca
     9361 tggccgacct ggcgcagcgg ctcgatgtca cgggcaggcg catcgtcgtg ctggccgggc
     9421 ccggtgaccg gcgcgatgag gatctggtcg cgatcgcccg cgccgtcggg ggacgcttcg
     9481 atcattacat ctgtcgccgg gacgacagtc tgcgcgatcg ggcaccggat gaagtgccgc
     9541 ggatccaggc ggccgcgctg cgcgcgagcg gcatcccgga ggaggccatc tccgtcattc
     9601 cagacgagca ggaggcgatt gagacggcac tgcgcatggg gcagacagga gatctgctgt
     9661 tgattttcgc cgatgcgctg gtgcgctcct ggaagcagat cacgaagttc aagcccgcag
     9721 gccatgccgc atccggcatg gagaccacct ccggaatgcc cggattgagc gcgcaggcgt
     9781 ccgccgcgtc cgcgaaaggc aacggccacg cgcgtccgcc actgctcggc ggcgagcccg
     9841 gtcagtttcc cgaagagcac cccgctccct ccatcagcct cgaaggtttg atccgagatg
     9901 agcgaggggt gcacgttgcg cgggagaccg aagactgaac ggccctccgg cactgccgtt
     9961 tgatctttca cgcaggctca cgggcgccaa cctgttcttc ccctacccgg gcgccgtcct
    10021 cgagctcgcg ggcgttctgc cggatgaaag actcctcgcg gcctggaaga gccgcgtcga
    10081 gcgtgcccgg gcgcgcttgg gctggccggc ttctcgcagc ggcggcccgg ccgtcgtggt
    10141 ggcgcgccct cattcgagcg gcgcctcgct ggcattggcg gctcccgtcg atcagctctt
    10201 cgtcgcgacg gaaatcaacg aatgggcgct ctgttctgcg cttcgtgacc gaaatccgtt
    10261 cgagtggagc ggactcgagc aagccctcgc cgccgctgca ttggagaacg ccgacgaccc
    10321 ggagcaggag atcccacccg cactggaaga gggagccgcc ttcgcgcgat tcgagcgcct
    10381 cgcgctgcag gaatccgacc cgcaattacg cgcgtttctg gatgcagccc aggcgcgcca
    10441 cctgcagcac tgcatggaca acgacatgct gaccctgggg agcggtgccg gaagcggaaa
    10501 ctttgcattg actcagctcc cgtctgttga gtcgctcgca tgggctgagt tgcacaacgt
    10561 gccgactgcg gtcgtgacgg gctcgaacgg caaaaccacg accgtgagac ttctcgccca
    10621 ttgcgcccag gctcatgggt ggctgccggc ctactgctgt acggagggcg tgttggtcga
    10681 tggcgaaagt ctggccgctg gggattactc gggtcctgcg ggagcccgga tggtgatacg
    10741 cgaacggcgt gcgcaggctg cgatcctgga aacggcgcgc ggcgggattc tgcgccgggg
    10801 cattgccgtc tcgcgagctg atgtcgcggt ggtgacgaac atcagctcgg atcatttcgg
    10861 cgagtatggg attgatgatc tcgatgcgct tgcagaggtc aagctgtcgg ttgcagcggt
    10921 cgttccagca gagggattgc tcatcctgaa tgccgacgat gcaatgctca ggcacaaggc
    10981 gaaaaccctc gcgcagcgat tcggtcgcct tccgccgctc gggtggttcg ctctggatgc
    11041 ggaaagcccc tttctgcagc aacaccgaat ccgcgggggt tggacctgcg gcgtgcgcga
    11101 tggacacctc cgcgcgcact accgcgggac cgatcacgat atcgggccga ttgcctcgat
    11161 gcccctgacg gtcgggggca aggcgaccta caacattgcg aatctggccg gggccgccct
    11221 ggccgcgatg gtcctgggag tccccgcgac gacgattgcg gcggtgttcg gccattttgg
    11281 cgccgatccc gcggacaaca tgggaaggat gatgcgtttc gagattggcg gggtgcacgt
    11341 cgtgttggac tacgcccaca atcctgccgg cctgcgtggc cttctgagtg tcgccggccc
    11401 gctgcgtacg cattccgcac ggctgggcct ggtgctcggt cacgccggca atcggcgcga
    11461 tgcggaaatc gagctgctcg cgaaggcggc agcggaattc cgcccggact tcatcgtcgt
    11521 gaaagagaac gagagccatc tgcgcggccg cgcgtcggga gaagtgccgc acctgatcca
    11581 tgcggccctg ctccgggaag ggatggtcga atcggcgctg tcgatgcgca tgaccgagct
    11641 cgaagccgcg cgccacgcgc tcgacctggc acacccggga gacgtgctgg tgctgcccgt
    11701 gcacgcggct gctgcgcgca cggcagtcct tgaaatgctg gaggcaaaaa aaagtgcgcc
    11761 cccgcgacag aattcccccg gtatcgcggc ggcgcacgag tcggccgaaa tcacaaaccc
    11821 ggataatggt ggttgagcgg cctgttggaa tcattgcgaa ttgtgcccca tcgcgacagt
    11881 gcgggttcta cgtaatctgg tcgacgcttg aaaaaaatgt gagacgcata ccgctgtgcg
    11941 cgcgatcggg aaggagtgga gacgatgagc gatctcgtgg atcgattcga tgccgagggt
    12001 tacgcgatcg ttcgcggatt tctctccgcg agcgagatgg cggagatcag tgcagaaaca
    12061 gacgcaatgt atcgcgaggg attgaagcat cacgcgagct atcgggatcg aaatcttcta
    12121 ttcgaggtgc tgaacgatcc gaaagcccgc cgccgcgttg ttctgcaggc gcactggacg
    12181 gcctggatca gcccgctgct cgagcgtatg cgccgcagtg agaagttctt cgcggtgctc
    12241 gagcccttcc ttggtgccga catccgccag atctctcacc agattcactg gaagccgccg
    12301 ggtgcaaagt acacttccta ccgcttccat caggatgcac gctttcgtca gggcaagctc
    12361 accgagcacg attacctgcg cagcagtgtg accacgggcc tcgcgatcga tccgcagacc
    12421 gctgacaacg gcgcgctgcg ccttttcccg cgcagccatc gactggggta cctcgggctt
    12481 tccgacgacg gtcccatcat ggtgggtgaa acccaggacg aggagttgca gcgcgccgga
    12541 ctcgatccct cggcagccgt gatctgcgag atgcaacccg gcgacctgct gctctggaac
    12601 ctcttcacgg tgcatggctc ggcgcccaat ctgagctcgc acgatcggcg tttcatgatc
    12661 aacagctacg tacgagcttc agacagcgac cgcggtgaat gggctttccg tggcggaatc
    12721 tcgacgccct tgggcacgga gcccgagatc tgcaagtacg agcagctgcg cgaacggccg
    12781 gggccgtttt acatcgaaga cgattggacg agagaagccg gcgcggaccg cgaggcaggg
    12841 tgattcgcgc gcagggcccg cgcgatcccg cggatcgcga ggaagtagtg cagcgaggcc
    12901 cagccgaaaa aggtgagggt gacgagcagg cccgcacgag tcgccagggc aagcggagtg
    12961 cccgccgccg tgaatcgatc gatcacccaa ccggtgaacg gggggccgcc tccgagggcc
    13021 aacacgctca gacagatgta caggagggcg gtggccgagg cgcgccgggt gggatcgacc
    13081 gcgttctgca ccacgccgaa ggtgggcccc agcgatgcat actgaaaaaa gcctgcgctg
    13141 ccgagcagga gggttgcgcc gggccagcgg ggttgcagca gcgcgagcag gtacagcggt
    13201 agggcgagca acgtgcccag ggccggcacg agggcatacc agtgcgcact gcggctcgcg
    13261 agccgatccg cgatgaatcc tccggccagg attccaagtc cgacggtcac tccaccggtc
    13321 atggcggcaa tgaagcctat ggtcgtgtaa tcgagctcga aggcgcggct gaagtaggcc
    13381 ggcataaagg cgtagaagcc gtaggccgcg aaggcgctga tcgtcacccc cagaaccata
    13441 tgaaggatgg gagcgctgag taacagcgag cggcccacgg cggcgagctc gtgcaactcg
    13501 actcgcagcg acagtggcgc gcgaatctgc gcctcgcggg gcggctcgcg tacggcgatc
    13561 ttcagcagca cggcgagcag cagtccgggc agcccgacga tggcgcaagc cgcgcgccag
    13621 ccccaatgct gggcgacgaa tccgccggct acagcagcca ggatgtagcc gaaggagatg
    13681 ccgcaggagt acaccgacag cgccgatgcg cgcctgccgg gctcgaagta gtcggacagc
    13741 agcgaatgcg ccggcggtgc acagccggct tccgcgaagc ccacccctac gcgggtcatc
    13801 agcagctgcg ggaaattccg cgccaggccg cacagggccg tcaggcagga ccagatcgcc
    13861 agtgagatgg ccatcagatg cacgcgattt acccgctcgg cgaggcgggc gataggaact
    13921 ccgccgaatg catacagggc ggcgaatgcc gtgccgctca cgaggcccag ttctgtatcc
    13981 gtaagaccga gggcgagctt catcggttgg ccgatgatgg tgatgatgtt gcggtccatc
    14041 gaattgaagg catagaccgt catcaacagc agcgtaaccc ggcgccggta gcgctcggag
    14101 tcgtcgatgg cctcccgccc tttcagtcga atgtcctgca gcggatctac ttctggttgg
    14161 cgtagatttt cacgagctgg tacaggaagg cgcggccttc caggagcgac ttcacactga
    14221 cgtactcgtt caggccgtgg atgtggccga ggtcaggccc gaggaacacg ggctcgatgc
    14281 cgtaggtcgg aatcccagcg gcgctgagga atctcccgtc agtcgcgccc ggctgcagga
    14341 tgggcagtat cggcacgccc ggccagaact gcgccgcgag cttctgcacc gggccgagga
    14401 tggcagccgt caggggtggg gctggcgggg gcggtggtgg tggcggtgca ccggggcttc
    14461 ggttctcgac aatagtgacc ttgacctcgg ggtcggcgac cagctcttcc agcttctgcc
    14521 gcacagcctc gggcggggtg cccgggaaaa tgcggcagtt gatgttggcg cgggcgcgct
    14581 gcggcagggc gttcgtggcg tgccccgcat cgagcatcgt cgcgacgcac gtcgtgcgca
    14641 tggtggcatt ccaggtcggg tccttggccg cgacgagcgc gatggcctcg ctgtcatccg
    14701 gattcttcac cagggcattc atggcattcg ccacatccgt ttcgcccttc gccgcttcga
    14761 ttcgcgccag gccgctgaaa taggcgcggc tcgcatcggt gaactgcggc gggaattcgt
    14821 aggcctcgat tttcttcaag gccccggcga gatgatagat ggcattgttc ttcacgggcc
    14881 gcgagctgtg accccccggg ttggtcacct ccaggcggaa attccgggaa gtcttctctc
    14941 cggcctggat gtcgaggacg atgcgcttgc cctgcgcatc gagctcgccg ctcgcaccct
    15001 cgttgatcgc gaactgcgca tcgatgagat cccggcggtt ttgcgtgagc cactcggcgc
    15061 cgttgaaggc gccgctggtt tcctctccgc aggtgagagc gagcttcagc gtacgtcgcg
    15121 gctgctggtg ggcttccttc aggcgtacca gcgtgtccac ccaaatcgcc gcttccgcct
    15181 tgtcatccat agagccgcgc gcgtagaagt tgccattctc ctccaccagc ttgaaggggt
    15241 cgcgtgtcca gtcttcgcgc ttcgcctcga ccacatcgag gtgcgccaga agcaggatcg
    15301 ccttgagttt cgggtccttc cccggataga tcgcaaccag gccgccttcc ttcgggtgct
    15361 cgggcgtcgc gaacgggtgc agttccgagt ccgggaatcc cgcagaccgc agatgcgccg
    15421 ccatgcgctc ggccgccaga gtgcagcttc cggaggacag actcgtgttg gtctctacga
    15481 gctccttgta aaggtcacgg aagctttgct cgctcgggtc gggtggctcg gcgagggccc
    15541 ctatgctcag cagggcgaat gcgaacgtga tcggcaacat gtttttcatt cgagacatcc
    15601 tgacaagatt cagccgggcc ggaccgtcgc caaccgaacg gagagaagat agaacagcgc
    15661 actgatgacg acgagtgcca gcgcgccgag aatctcggtg cgcgaggcca gggagatcat
    15721 cgcgaccatg ctgccgatcc cgacgaatgc ggcgggcccg agccagcgtg agttcagcgg
    15781 cggcccggcc tgcgcgacct cgcgacgggc gagccgatac gcagccgcac acccgctccc
    15841 gtacaacagt gccgaagcga gggtgcctag aaccgccagc tcagcgaaag agcccgataa
    15901 agcaagcacg atggccaggg ccgcatagac cagaatggcc acatacggcg tgtgagtcct
    15961 cttgtgcacg cgccccagga tgctgaagag cagaccatcg cgcgcgaagg cgaacagcat
    16021 gcgcgggctg ccgagaaagt cgctgctcag ccagccgagc atcgacacgg acgccgcgac
    16081 cagcatcatc cagcgcaaag ccgggctgat ctgcgccatc gcatccgcca gaggagtcgt
    16141 ggactgagcc agggcaggac cgaggattcc ctgcgccacc gcctggatcg agatgtaaag
    16201 cacggtcacg gccgaaatcg acagggcgag ggctcgcgga atggagcggg cgggctgcgc
    16261 gacttcgccg ctggcgctca gcgtgacttc catgccggtg agggtaaaca gggccaacag
    16321 gattccacgg tcgaagccct gcggcgtggg aggggcggtc acaacgaagc ccccaccgtg
    16381 ccaggcaaaa gcgccggcca cgatgaaaat ggtcagcggc agcagcttga ggaccgttgc
    16441 gatgctggcc aaccgggccc cctgcgcagc gcccgcgatg ttgaccagcg cgatgcctcc
    16501 gacgaggccc acgatgctcg ccgcatgaac gacaggagcg tacgacgcag gcaccaggct
    16561 ggcgatgagg tcagccaggg cagccgcgac acctccacag gcgagcacgt tgccgaacca
    16621 aagcaccgtg ccggccacgt aaccgaccag cgggccgaag gccacctcga tatagccgta
    16681 gatgccgccg ctggtcggaa tccgacttcc tccttcggcc aggcacagtg caaccgatcc
    16741 gatggcaatc ccacaaacca cgaatgccag cggtgagtag acgcccatcg aggcggatag
    16801 ggccgccggg accacgaaga ttcccgcccc gatgatcccg ttgaagatgc ttgcggcgaa
    16861 ggtcagtggc cctaccgccc gcagcaaccc tcggtcacgt tggttctcgg tcattcgttc
    16921 aaccgtgacg tcaggtccgg gcagggcaat gatttcggct ctttcttgta cacttgcacg
    16981 ccctgtccgt tgcacccggg aggatccatc atgcttcgct ggttgtccat tcccttcttt
    17041 cttgcggtgg ccatgcttgc cgatgcccag agcgcaccgc cagcctccag tgccggcccg
    17101 gcccacccga tcctcatcca tacccagtcg ctgatcgatg gcacgagcgc gcaggtgcgc
    17161 cacggacagg acatcctgat tcgcggcaat cggatcgtcg aggtctcccc ggccggcgcg
    17221 catgcggcgc cggcggatgc cgagatcatc gagctcggaa ccgccaccgt ccttcccggc
    17281 ctgatcgatt cacacactca catctttctt cagggtgaag tgccggaggc tggggggtat
    17341 gacgtgcagc tgctgaaatt tccagcctcg taccgcgtcg cgcgcgctat cgtggcggca
    17401 cgccgggccc tcgagcaggg attcacgacc atccgtgatg tggagaccga aggtgcgggg
    17461 tatggcgatg tggggatcaa gcgcgccatc aacgagggct acattcccgg gccgcgaatg
    17521 ttcgtggcca cccgcgccat ctcctcgacg gggggctatt cactcgatga ctacgcgccg
    17581 gagatcaccg tgccaaaagg ggcgcagatc atcgatggcc cggtcgaggc tcgcaaagct
    17641 gcccgcgagc agctcgacca tggggctgat tggatcaagg tctacatgac gcatcgatcg
    17701 tgggtcgatg agcagggcga gctcgtgtcg caacccactt tgacggttga ggagctcaag
    17761 gcgatcgtgg acgaggccca cggctggcgc cgcaaagtgg cctgccacgc ctataacgga
    17821 atcggcctgc agcgagctct ggatggcggc tgcgattcca tcgagcatgg tctcgagatt
    17881 acggacgcgc agatcgcgca gatgatccat caaggcacct ggtacgtgcc gacactggcc
    17941 gtttactact acgatcccga tccaccggac acgccgagtg gcaagcggga tctcaaacgc
    18001 atctccgacc acgtcatctc gttcaagaaa gctctgcacg ccggcgtcaa gatcgccttc
    18061 ggaaccgacg tgggcggctt tgtctggacc gatccaatcg cccaggaatt cgttcgcgag
    18121 gtggagctgg gaatgacccc gatgcaagcc attcagtccg cgacctcgca cggggcggag
    18181 ttgctcggca agcgcggtga gctgggcgtc atcgcaccgg gagccatcgc ggacgtgatc
    18241 gccgtgcccg gcgatccgct ccaggacatt cacctcatgg agaaggtgca gttcgtcatg
    18301 aaggagggtg tcgtgttccg ttcggcatcg ggcgcgacgc acaccccttg agggggagct
    18361 ctgcgctctt gggcggcgtg cggagtgctc acatgatcgc cgggccgaat acacagccct
    18421 gtggggcact gagtccggta gccgagccga tgaacaacgt gtcaggagtg ggcgcaccgg
    18481 ttgcgagttg gctctttgcg aacagagcca ctgcgaaagg gcctgcggag ctcaccgcga
    18541 cgagattgcc ctgatcatcg aaacacagac catgcggtgc atccagcgcc gcattcccgc
    18601 tgacggtggt cgagctgagg atggccgcgg gcgtcggctt gccggaggtt gcaaggctcg
    18661 atttcgcgaa ctcgaccacc gtggcggaga cggcattgct cgaccagagg ttgccgtcgg
    18721 catcgaaggc cagggcccac ggaccctgaa tggatgagtc gccgtcatcg ttcaaagtga
    18781 tgtcgggggc gagatcgggc gtcagcattc ctgtcgtagg cggtgtcggc agcttggcgg
    18841 cggcaaactc cacaatcgtc gtgccagcgg agctcgtccc gccgctgctt tgcggtacgt
    18901 ctccatcgtt cgccacccag aggttaccgg cgccgtcgaa tgcaacaccg gtcgggccgt
    18961 tgagcgctgt ggaggagatg atgaccgcgg gtgagatgtt gagtccggtc tgccccagct
    19021 gcgccgccgt gaagaccagt atcgtgttgt tgtcgtgatc ggtgatccac tgatccccct
    19081 gcgagtcgaa cacggattgc tgcggcagct tgagcgcagt cgaggtgatc acggtcacag
    19141 gctcgggagc gttgctggtc gcgagcgcgc cgacctggga ggcgctgaag ccgagcaccg
    19201 ccgcagtcgc gaccccattc accatggcag cggaatcgag cacccacaga ttaccgttgg
    19261 catcgaactg aacaccctgc ggcgatccga aagcgccgct gctgacgacg agcggcccga
    19321 cattcgcggt gccaccgatc atctggttgg ggacgtactc gagcacgttg gtaccgtttg
    19381 cgatccagag ccccttcgtg cccagcgtgg cgtgctggtt cgtgatcgag ccttgcccgt
    19441 cgtacttgca ggatgctgcc agtgcggctg ccaggatcac gagcggtaaa gccagaacga
    19501 ggaccggccg tatccggcgc tttgcgtgat tgctcatcga gacagcctct cgcagatgct
    19561 tgggagccgt gccgctgccg atgggacagc ggccccgatc acatggtgag ggtgacgcgg
    19621 gattcagaca tagtcaatga gggccatgaa gccgaaatcc atgtgcagct gctggtggca
    19681 gtgaaacagc gtcagcccgg gattgtccgc gatgaaatcg acctccacct gctgataacc
    19741 gcccaccatc accacgtcct tcatcacgcc tgcggtcggc ttgcccgcaa ggctcaccag
    19801 ctcgaaactg tggcggtgga ggtggacggg atggatgtcg tcgctcgcat tgcgcatctg
    19861 caggcggtag cggcggccgg cgtgaacctt gaacagcggg ctcatggcct tcatcgagaa
    19921 ggactcgccg ttcaccgtcc actggttgaa gccgctgcgt gccgcgttga cctttgcgaa
    19981 taccattgga atggtctcat cgggcgcgtg cgctgtgccg cggccgaagc gcgtgtaatc
    20041 ccagcggctc gatgccggcg cgccccactt cggggtgcct cttgcgcccg cgtattcgac
    20101 cacgatgccc atcccgttgc gccgatcgtc gtccgccagg tcacccagaa tccacactcc
    20161 ggggcgattc atctcgacga tcgccgaaac cctttcggcc gtgccgagcc agagagcatc
    20221 gacgcgcgcc ggtacgggca cgctattgcc gtcgagtgcg acgaccgtga aggtatgtcc
    20281 gggcagcgcc aggctgcgga tctccgtggc gctggcattg agcacatgaa agagcacgcg
    20341 ctcgccggct ttgacacgta ccggttcgcc atgtcccaac atgcggccgt tgatgctgaa
    20401 gtacttgtag ccgacctcgt aaccgtgcgg catgcgccgc cggagcgagg cgttcatggc
    20461 cgattcgccc gcgcgcttca gctcgggatc ggtccgcgag ggctcgagaa agtgcatcgc
    20521 catgtcgccg ccctgcgaga gggcaggctc gaactccttg agcaccagga tgacctcgcg
    20581 gtcgtaatgg ccgggctcgt gagccgcctc gatgaggacc gggccggcct ggccgctgta
    20641 gaggccgccc tgcagattcg accccgcgat caggtgcgtg tgatagaaac gcagccccgc
    20701 cggccccggg gtgaacgcga tccgccgcat gcctcttgcg gcaatgtagg gagtgccttc
    20761 ctcggccgcc ccatccacct caacgggcac ggtttgtccg tgccagtgaa gctgctctgc
    20821 cgtctccgta tcattatgga tgtcaacgac cacgctgcga ccctgtttga agcgcagcag
    20881 cggacccggg aattgtccgt tgtaagtcgt agtcgagatg acatgatcgg gcgccagctc
    20941 gatgttggat cgtgcaatgc gaatcgtgta atccgcccgc atgtcggccg gatgatcgcg
    21001 tggggcggtc gcaggtgaaa ccggcggcgc ttgcacctgc gcgagaaggg gtaacccggg
    21061 ggcaagcgcc gcaagtcccg acaaccttag gaagtcgcgc cgctccagcc tcatatcgtc
    21121 aagccaccga tgacgccaag ctcaggcaaa cttcgtccat cgactcatca taccgcaccg
    21181 cccggtgctc gcgcgcagca ccgcctgctc acgcaacccc gcttcgttcg cgcgcaccgg
    21241 gtcacgatta ttagcagaaa gcgggcacga acggactctg gccggcgcag ccgcccgcat
    21301 ggacactgtg atcctccatt cagggaggaa tccagatgtc atcccatctg catgcgcgcc
    21361 gggaaggcgc gctcggggtt ctgttggttt cgtgtcttgc ggtggcgggc ctcggccggg
    21421 cggccacggc tccttatccc gacagtacgc tgatcagcgc catgagctgg gacttcagca
    21481 gcgtcgtgac gctgcgcaag gctcacggca gcgacctgtg gccgctgacc tgggcggcgg
    21541 atggcaatct gtatggagcg tggggcgacg gcggcggatt cgacggcgac aacaacaaca
    21601 tcgggcgcgt gagcctcggg tttgcgctcc tttcagggac gccggcctcc ggcgaacctg
    21661 acagctatgg cgggcgcaat gtgtggggtg cggctccccg gttcgccgag catcgggcca
    21721 ctttcggcgg caaggtcgat gatctgatct caattgacgg cgtgctgtac ggacaagggg
    21781 gtttgtggac ggcagccaac tgtggctgcg cggatcccac tctgaagccg ggtgacaatc
    21841 gacaccaccg gaccgtaacc tggtcggcgg atcacggccg cagttgggag gtggccgcct
    21901 ggacgagctc ttcgggacct ggcacgtccc tgcaatacgg acgcaactat cagggcgcca
    21961 aggatcctgc ccacgtgtac ttttacttcc agcccgacag tgcgagtgat gccacccgca
    22021 tctaccttcg cagggtgttg acgagtcagc tcactgccga tcccgccacg ccgggccatt
    22081 tcgagtatct gacggcgatc gacggggagg gatcgggact ttggtcggcc agccagacga
    22141 gcgcactccc cgtgttcgaa gattcccatg tcccgcccgg caccgatgcc aatgcgacgg
    22201 tggtgtacga tgaggggctc ggccgctatc tgatggccac ccaacacggt aacggggccg
    22261 gtcagatggg cttcttcgag gcgcccgcgc cctggggccc ctggcgaacg ctgggatact
    22321 acgaggattg gggcggcttc aacgagagcg ccggagaagg aaacggcctg tcgtttccct
    22381 ccaagtggat gagttcggac ggccggacgc tctgggcggt gttctccggt gccggaacgt
    22441 ttgattcctt caacgtggtc aaggcggtcc tcaccgtcgg cgcagagatt ccgcagattg
    22501 ccgacccggc cgccggaacc gtactggctc cgggcgaacg cgtgagtgcc accggagtcg
    22561 gtacgggcct atcctggtcg gtcgagagtc tcgggaagac agagtcgttg gtcgcgacgg
    22621 gcacaggacc cagcctgaca ttcatcgtgc ctgaaggtgc tccggcaggc tcgctgatcc
    22681 gtgtcaccct cagcaatacc ctgacgcagg tctcgcgcga ctatgccgta tcaggtcggt
    22741 ccggcagcct gctcgttgga gactggatgt tcgacgaagg gaccgggagc accacacggg
    22801 actcctcagg ggagggcaac accgggacgc tcgtgaacgg ccccgtctgg gtggccggca
    22861 aaacaggtca cgcgctggac tttcgtgggg gccgtgccgg cgtccgcgtg cctggcgttc
    22921 ccactcttgc gaacctgttc gcaagaggtc tgaccgtgat ggcctgggtc catccccgct
    22981 cggacggcaa cagcggccgg atcctcgaca aggatggcaa ttcgcggggc tggctgctga
    23041 aaatgagtga gggcggcgtg cagttcgtgg gtgacgagtt cggcgtgacc gcggtgagcc
    23101 gaaaaacgaa tcgctttctt tcgagaaacg cttggcagca cgtcgcagcc acatgggacg
    23161 gttcgtacaa aggagccgac atacacctct atgttgacgg ggttcctgcc gatggcacct
    23221 acgtcaacgg cggcggagga ctgcgtgatg actccgcaat tccgctgacc ctgggcaatc
    23281 gcctgttcga tctggagcgc ggcttcgacg gaatcctcga tgacgtgcgc atctacaatc
    23341 gcgtcctgac accgggtgag gttcacgagg tggccatggc ggcggtttcg ccttcgaccc
    23401 gcgcgccatg agcgcggtgc cggtacctcg tttactttga agccgattgc agcgccgggg
    23461 acgtggtcag aaacgtggag gcgacatgag ggacgagggc ccgtacgcca tccagcacac
    23521 aggagcatcc ggcgaagagg agtgcatccc gggaaaagcc gcctgtgaga acgccgaccg
    23581 gttccagtcc cagtgcgctt gccgccctcg catcgtaggg ggtgtctcca agcgtcagga
    23641 cctcggacgg ctcggtcacg cgcagtttct tcagaacggc tcgaaacagg tcaggatgag
    23701 gtttgccatg cggcacgtca tcgccgcagg tcacgacatc ggtcagcgcc aatacgcgca
    23761 tctgcttgtc atagaccgcg agctcgtcgc gcgtgcaggt ggtggcgatg gccagcgcca
    23821 ggcctgcgcg cttgagggcg acgaaaaccc ggcggacccc ttcgaacggc cgcactgcgc
    23881 gcaggtgctg gtcccggtac agctcaccct ggcgcttcgc gatggactgt ctgccctcct
    23941 tggacagcgc cggaagcaga ccgtcgagca tctcttcgcc atccattccc gaatacggct
    24001 ggagctcgtc gcgtcgaaat gagtgtccgg attctgcgag cgactggcgc cagctctcca
    24061 gggtctgcgg cacgcagtca accaacgtcc cttccacgtc gaaaattact gttgtggtag
    24121 atctgttcat tgcacgtcga gtcagagctc ctggtaaacg tgccgtgcag ctggatagtt
    24181 ccgcctcagc ccggctgccg cggttgtcag gtggggctca gagtgggcaa agggggcaac
    24241 aaacagcagc tcgattcgct gtctgttagg ccctggcgag ggctgaccgg gcagcgtgcg
    24301 gcggcacgtc gattgctgga tccagggtcc cctggggtgt gaggtgccag tcatgactag
    24361 agttgcaagc acgtgtgcgg ccactttgat gtgcggtttg atcggtgtcg ctttcgcagg
    24421 caatcaatcc gccgacacgc cgtcttcgga gagcgctgcg acgggccaga cgagaaccga
    24481 cggcgcttcc ggtcggatgt cgatgagcaa atcgcttact gatcagtcct ttgtcaccca
    24541 ggctgcgcag ggcggcatgg ccgagatcga tctttcgaat ctcgcgctgc agaaatcaca
    24601 ggacagtcag attcgctcgt tcgcgcagcg aatggtgcag gatcatggca aagccaactc
    24661 ggagctgaag cccatcgcgc agaagctcgg gctgcaggtg ccttcagaca ccgatgccaa
    24721 gcataagcag gagatgcaga agcttgggga ggaatcgggt gccaagttcg atgcggctta
    24781 ttcgaaggcc atggagaagg atcacaccaa ggccgtcgag ctgttcacga aggcgagcaa
    24841 gtccgacacg ctgaagcccg atctgcgcaa ctttgccgcg aagacgctgc cggtcctgga
    24901 gacccaccac cagatggccg acaacctccc caagagctcc atgcgctcgg cgcagtcgtc
    24961 caactcgcag ccgcgatcgc aatgagagtg gccgccctcg agcgagagct tgaggttgat
    25021 cagggcactt cttgagcgcg gactgagtcc cgctcagtgg gtacaagcgg cccaggcctt
    25081 ccggatgccg gagcctgggc cgttcttggt cacacggttg gcggggtccg ctgaacgctc
    25141 accctgaccg cgcaccttca tcgccggtgc tctgcatctg cgcttccagc agctcggagg
    25201 cttccagcca ggcggactcc atctgctcgg tctcccgcct gatcgtcgcc tgtcgcgcaa
    25261 gcagatcgcg cagccgatcc ttctcttcat tcgcatacag ccccggctcg gcgagcgcgg
    25321 cctgaagggc tgaggcctcc gcagtcaatc gtgcgagctc cgtatcgcat tgggcgacgc
    25381 gtgccttgag tggcgtcagt cgatttcgcc gctcggcctc ctgccgccgg cgctgtcggg
    25441 ccgccgcgga gccgctgatc gcttcgctcg ccggctcctg cgcggcgtcg gccatcgagc
    25501 ccacggaacg ctccatgagc cacttcgcat aatcctccag gtcgccctcg aagggtgtga
    25561 ctcggccccc gtggacgatc tgaaaccggt cggcgacggt tcgcagcaga tggcggtcgt
    25621 gcgacacgag caccacggcg ccggcgaagt cctgcaaggc caccgcaagc gcctgccgca
    25681 tctccaggtc cagatgattc gtcggctcat cgagcaacag caggctgggc cgctgataag
    25741 tgatcagcgc caggaccaat cgcgccttct ctccgcccga aaaaggtgcc acgggctcga
    25801 acacgcgctc gccgcggaag ccgaaaccgg cgagaaactc gcgtagctcc tgctcggtag
    25861 ccgccgccgc ccgctccata cccgtccggc gcaaactccc gagaatcgta tcctccccac
    25921 gaagctgctc cagctggtgc tgcgcgaaat agccgatgcg cagctcgcgc gcctcggtcc
    25981 gcaggcccga gaggccggtc agctcgcctg ctagcagctt catgcaggtc gattttccgg
    26041 caccgttgcg cccgaggagg gctacgcgat cgcccggcgc cagtgtcata ttcacctcct
    26101 cgagcaccgc gtggccgtca tatccaacgg actgaccttc cagtgtcagc agcggtcgcg
    26161 gcagcttgtc gggttgcgcg aaagagaact cgaaaggcga gtcgacatgt gcgggtgcga
    26221 tgcgctgcat gcgctcaaga gccttgagtc ggctctgggc ctggcgcgcc ttgctcgctt
    26281 tcgcgcggaa tcgctcgatg aacgattcca tatgtttgat ctcgcgctgc tggcgggcat
    26341 acagcgtcga ttgctcggcc agctccgcgg cgcgctgctc ctcgaaagcg gagtagttgc
    26401 cccgataggc acgcgcatgc ccatgctcca gattcacgac ccgttgcacg acacgatcga
    26461 ggaactcgcg atcgtgcgcg atcagcagca gcgtacccgg ataatccagc agccagctct
    26521 cgagccacag gatggcgtcg agatcgaggt ggttggtcgg ctcatccagc agcagaaggt
    26581 cggagggaca catgagcgcc tgcgccagat tcagccgcac acgccagccg cccgagaacg
    26641 cgtggacggg gcgcaattca tcggcagagc caaatccaag cccatgcaga agccgcccgg
    26701 ctcgactgcg ggcttcatac ccgcccgccg ccgcgtattg cgcgtaaagc tctcccagac
    26761 ggattccgtc atcgcgtgcc tgcgctgctg tgatggaccg ctctacggcc cgcaaggccg
    26821 catcaccatc cagaacgaat tcgatggcgg ccgtctcgag cggcagcagt tcctggacta
    26881 cgtgcgcaat cgcgaggttc gagggcagct cgaaagtgcc ggcatcgggc tgcagctcgc
    26941 ctcgcacgag ccccagcagg ctcgacttgc cgctgccgtt ctcacccgtg atcccgacct
    27001 tctcgccccg gaagatcccg aacgtggcgc cggagaacag cacgcgcgtg cctcggcgca
    27061 aagtgacatc ggtgaaattg atcatcggag gatgtgtaat gatagggcca ccggaactcc
    27121 gcggatactc tcgtgacctc cccgacacgc aagccgcgag tgatcgatct cacccagccg
    27181 ctcgggccgg cgaccgtcat gtggccggga tcgagtccgc cgtcctttcg cacggtggat
    27241 gactaccgtg aagcgggctg cttcagtcgc caggtcagcc tccacgaaca taccggcacg
    27301 cacctggatg cgcccgcgca cttcatcgaa ggtgcgcaga ccgtggacca gattccagtc
    27361 gagcagcttg tgtgcccggt agcggtgatc gacatcgagc cgcacgcggc gcgcgacccg
    27421 gactacgctc tgtcgatcgc ggacatcgag cagaatgagg cgaggcacgg cgagattccg
    27481 cgaggcagtg ccgtcatggt gcactcaggg tgggcgaagc acgtggccaa tgctcgagcc
    27541 tacctgggtg agcggggcga tgggggcctg aggtttccgg gagtccgctc ggcggccgcg
    27601 ctgtggctga tccggcaccg tgagatcacg gggctcggca tcgatacgcc ggggatcgat
    27661 ccgggtgagg acactgagtt cactgtccac cgccatgcca cgctacccaa tggcctctgg
    27721 cagctcgagg ggctcgtcaa tctggatcag ctgccggcgc ggggcgcaat ggtcttcgtc
    27781 ggcgtactac cgttcgtcgg aggctccggc gcgccggccc gcgtcatcgc gctggtctga
    27841 tgggtagcct gatgacgcat tgcgccccgg aaatacgata aaggagtgcg gcgaactcac
    27901 aagcgccgat acggggagtg agatgtcggg tctaaccgag gaacaatggc gcctgttcag
    27961 cgataccctg ggtgaggcgc tcgagatgcc cgagtccgag cgggccagct ggctggctgg
    28021 agtggctggc accagtcccg aagtcgccgc cgccgtacag cgggccctgg ccgcgcggga
    28081 tcgatccgga ttcgcaaatt ttctctccgg tcccttgctg gacagcagcc ctcccgctga
    28141 agtgacgctc gtcgggcggc gagtcggccc ttacgtcatc gagtccgaga tcggccgcgg
    28201 cggcatgggc agcgtctggc gagcccgcag aaccgacggt cgattcgagg gcacggtggc
    28261 gatcaagttc gtccatcctt actggatcgg gcacaccggt gaggaacgct tccgcgcgga
    28321 aggcctgatg ctgggacggc tggatcatcc caacatcgcg cggctcatcg atgccggagt
    28381 catcgatggc atgcagcctt acctcgtgct cgagtatgtc gaaggcgagc cgatcgacat
    28441 ctattgcaac cgcctgcagc ttggcgtgca ggcgcgcgtg atgttgttcc tgggcgtgct
    28501 cgccgcagtg gcgcacgcgc acagccatct catcatccat cgcgatctga agcccgccaa
    28561 catctttgtc acgcgccatg gctcggtcaa gctgctggat ttcggcatcg cgaagctgct
    28621 gagcgaggag ccggcccgaa caccgaccca gactctcggc caggcgctga caccccaatt
    28681 cgcagcgccc gagcagctcc tcggtccgac agtgacgacc gccaccgatg tgtacgctct
    28741 gggcgtcgtg ctcttcgtgc tgctgaccgg tgagctgccg atccaggtca gctcgcgttc
    28801 ccaggccgag ctcatccatg cggttctgac ggaggaggcg ccacgagcct ccacgatcac
    28861 gagcctcact ctgccgcacc ggcgcgccct cgggggcgac ctcgataaca tcctcggcaa
    28921 ggcgctgagg aagaatccgg aggagcggta cgcctccgtc ggcgcgctcg ctgaggacct
    28981 gcgccgcttt ctctccgacg agccggtcag cgcccgtccc gacacggtgc cctaccgggt
    29041 cgcaaagttc gttcgccgca accgcggcag tgtcgcgagt gggctgctga ttgcgattgg
    29101 tctgatcggc accagcgtct tcgccctgac acagatgagc gccgcccggg cccggcggga
    29161 tgtggcgctg gaggaggcca aacgggccaa cgcgcaggcg gatctgactc agtacctgct
    29221 ggatgacagg ctgagcagac tctctcccga ggccgagcgc cagcggctcg agcgagcgcg
    29281 ccgcttcgtg gcggcccgat tccgcgccga tcctctgcac gccgccccac tgctgatcga
    29341 tgtgtcgggc cgctacatcg acatcggtga ctttcgcacc gcggcgcagg tcatcgtcga
    29401 ggtggaagca atcggtcgtc agttcgatga tgcggcgatt ctcggacagc tcgcctgcct
    29461 gcgtacgcag gacctggcca tcgcgcgcga cttcacgtcg gcccgcctgc aactcaccac
    29521 tgggctggcg cagatgcagc gtctgcaccc cgtaccgctg gggctcgaag cggaatgcgc
    29581 gaccgccgaa gcctttgtcg tccagcagga cggtgatttt gctcgcgcgg tgaatcggct
    29641 gcgttcgacc gtcgccgatc tggacaaggg agggatgcat ggagcaggcc gttacaccgc
    29701 tgcaaccaac gacctgacgc gggcgctctc catggccggt caataccacg atgcctggga
    29761 agtgggcagc cgcaacgtga ctctggccag cgatctcgga cgagccgata ccggcggcta
    29821 tttcgcctat gtcgccaatg cctgctcggc gctggtgaac ggcggtcaac cgattcgcgc
    29881 gctcgcttac ctgcaggcac acaccgacag tgtggcgcac gaagctgact tcagcggcat
    29941 gtccacgggc atccgcggct gctgggcact tgcgcaattg cagatgggcc acgctgcagc
    30001 ggccgacccc accctggtcg agatcgcaga caaggccgaa cagtccggaa tcagctacca
    30061 ggcttcgctt taccacgcgc tggcggtcaa gtccgccctg gcccgcgggg atctcgccac
    30121 agcgcagctg cgctggcaat cgctcgcctc cgacgaggag catcgtcttg caaccggtga
    30181 gcgcggaatc gaggtcgttc gactgctcct ggtgcaggcg cgggtcgagc ttgcgcaagg
    30241 tcggccgcaa gaggcactcc attccctcga tcgtgcggca gggctggttg ccgaacgcca
    30301 ccagccgacc aatcccgact ctcgcgagct cgaagagctg cgcagccgtg cgctgctggc
    30361 gcagcggcac tacggcgaag ccgcaagaca ggcgcagtcg gcgattcaac tcgcgcagac
    30421 gagcgccgtg gatgcgaagt cgtcggccta cgtcggggaa gctctcctgc tcctggcgcg
    30481 cgcccaggca gcttcccggg atcaacgcgc ctcgatcagc gctcaaagcg cgttgccgca
    30541 cctgctgacc aacctggatc ccacccatcc tctcatcgcc gaagcgcgcc agctcgaagc
    30601 gggctcgcct gcgcctgcgg gctcgccgga atgatcgcgt gacgggcgaa ccgctgatgt
    30661 cgcgcagccg cggcagctca ccctggcgct ggtgggtact ggccctggcc gcagtcgcgg
    30721 tgtcgagcag ctactacgag tccgatgtga tcgggcccat cgccgatctt ctgcaccggc
    30781 agcgcggctt cacccagtcg cagctcggga tgctgaacgg catcatcagc attccgaatg
    30841 tcgcactcgc cttcttcagc ggattgttga tcgatcgctt cggggcggcg cgcgttgcgc
    30901 tgtggaccgc tgccgtcggc atgctcggcg cttcacagcg atcggcactc cctacacact
    30961 gatggcggcc ggccgtcagc tggccggcaa ccacgctcat cgtcgagccc aaacggctcg
    31021 gcacggcgct cgggctcatc acgctcattc aggcactcgg catggctgcg gccaacctgg
    31081 ttgcaggcgg gcttgccgat cgcgccggcg ccgggccggc caacgcaggc ggctacgcgc
    31141 ccatgatggg attcttcttc gtgttgagcc tggtcgcgct ctcctcggcg gtgctgctgt
    31201 ggcgccggga aagaggtccg cacggacacg gtctggagga tgctagcgcg cgatcgcggg
    31261 agctgtacga aatcgagccg ggttgatccc ggtataaaaa aaggcccggc tgcacgtccg
    31321 ggcctgctta agcaagcgcg cgcgttacca gcggtcgcgt ccgccgccac cgccactgtt
    31381 gccgccgcgg aacccgccac caccgccggg acgcccgccg ccaccgctgc gcggcttgtc
    31441 ctgcgcctca ttcacgcgca gctgacgacc gcccatatcg tggccgttca ggctctggat
    31501 cgcacgagcc gcatcagcct ggctcatctc gacgaagcca aagccccgcg gccgtccggt
    31561 ctcacgatcg gtcggcagcg atacggactc gacagtcccg tgctgtgaaa acaaggcccg
    31621 cacgtccgcc tcactggcgc tgaacggcag gttccccaca tagatcttgg ccataaagtt
    31681 agcactctcc aaaacaactc aaacacggca ggaacatcag tcaggtcagg gagagtgtgt
    31741 ctcacgagcc catcgaaccg taggtcctgt cacccgatcg cgttccattc gcgccggccc
    31801 cccctcgatg tcttcgaccg atacgggact gagcccggac attccgcgat cggcgcaaag
    31861 tatgccagaa gctacgcctt ggtaccgcgc ggaagaatgc cttcccggct ttcgaagagt
    31921 caggggcgcc cgccgatagg gatcggtttc attgtcgtgt cacaaaagca acgtcggacc
    31981 ggacgcgccg cagcctccgg gacgcccgtc tggcgcaacg aattctatat atttcagata
    32041 gttagatgca ggcggcggtc gccgcgcgat gttcccgcca ggatcggcgg gcggccggag
    32101 cagtctggcg ctggaccgag ggctaacacc gaggacgtgg acaacaatgc tcctcacacg
    32161 cttgcgcgga tgtttacatg tgttgcgtta gggacctcgc gggccaaagc gttgcccgat
    32221 cggctgcagt ggccccctgc atcgcttgcc atctgcctgc cgcaatggaa ggcttcggcc
    32281 tgtgaacgac agcgccggta tggagagtat cgcctttggc gcccccggca tcgcccccac
    32341 ctggtgttcc agcgacaagg acttcgtcac gacggcgctc ggcgcttcac gtctatgggt
    32401 gaccatcggt cacggaatca tcaacgagat ctactggcct tccaccggcg agccacaggt
    32461 tcgcgatttt ggcttttacc tggtcggcgc gaaccgctgg ctcgatctga agcgcctgca
    32521 gcgctatcac ctggtgacgc cgggaccctt tctgccgctg ctgtccatca cgcattacgg
    32581 cgtggactat cggctggtgc tggagatcct tcccgatccg cgtcgtgatg tgctgctgat
    32641 ccgtttcgag ctcgacggtc cgtaccggct cgtggtcatc ctcgcgccgc atctgggcag
    32701 caccggtcgg gacaattcgg cgtgggtgga gcactccgtc gggtacgcct gtcgcgggga
    32761 tgcagcggtg tgtctcgcgg cggatgctcc gctgctgcat atgagtgcag gctacgtcgg
    32821 ctcctccgat ggctggcagg atctggagcg ccacacaaag ctcacctact cattcaccca
    32881 tgcgccgcag ggtacggtcg cattgaccgc cgaagctcgc ggccgcagcg gtgtgctcgc
    32941 gctcggcttc tccgagtcgg cccgcggcgc acacacactc ggacgcacag cgctggcgat
    33001 gggcttcgat gcgctgcgcg ccgagtttct ccaggcctgg cgaacctggg gatcgcaact
    33061 gcagcttccc agtcccgatg ccacgctggc ccacgcgggt ctgctgtccg ccacggtgtt
    33121 gaagatccat gaggaccgaa gctatccggg tgccgtggtc gccagtctca gcgtgccctg
    33181 gggtaacagc acggatgcgt tggggggcta ccatctggtg tggccgcggg atgccaccct
    33241 gaccgcattc gcactgctcg ccgccaatca gtacaacgac gcccgccata ttctcgccca
    33301 cctggcggcg agccagtccg ctgacggccg ctggccgcag aactacttcc cgagtggcac
    33361 cccctactgg acgggcgtgc aactcgatga gacttctttc ccggtgctgc tcgcggcgaa
    33421 gctgcgcgag ctcggcgaag aggatctgcc cggcacggcg cacatgatcc gcgccgccgt
    33481 cggattcatc gtgcgcaccg gaccggccag cgatcaggac cggtgggagg aaaaccccgg
    33541 cgtcagtcca ttcacgctgg cggtcgcaat cagcgccctc gtggccgctg caccgtggct
    33601 cacggatgag gaaagggagt tcgccgatgc cctcgccgat gactggaacg agcggctcga
    33661 gagctggtgt tatgtacgcg acacgcctct tgcgcgcgag ctgggcgtga ccggttacta
    33721 cgtgcgcatt gcctcgatcg ataaatcggg cgagctcgct gcgcaggtcc ggctgcgcaa
    33781 tcgagccggg gagatgattc gggcttgcgc gctggtgagc atggatttct cctacctggt
    33841 gcggctcggg ctgcgcagcg cgcgcgatcc gcgcatccag gacacgttgg tggtcgtcga
    33901 tcgcgtgttg cgggtgcaga cgccctcggg cggcatctac catcgctaca acgaagatgg
    33961 ttacggcgag tacccggatg gtcgtgcctt cgatggcagt ggcgtggggc gagcgtggcc
    34021 cctgctcgtt ggcgagcgtg gccatctggc catgcaggcc ggagaggatc cgctgcccta
    34081 tctgcacacg atgtggaact gcgccagcgc cgggggcctc cttccggagc aggtctggga
    34141 ctcggacccg attccgcacc tcgagctgat accggggcgc ccttcgggca gcgccatgcc
    34201 actcctgtgg gcgcacgccg agttcctgaa gttgctggtg gcgcgcgagc gcggccgtcc
    34261 gatcgagatg ctgcagtccg tggagcagcg ctatgcaggc tcacagagcc gcagcgcgca
    34321 ggactggcgc tggcgcgagg aagtgccgct ggcgaggctc gaggcgggcc gtggcctgct
    34381 gatcgaggac cgcgagccct tcaccgctca tctcggattt gatgactggc agggtctcga
    34441 ggatcggccg gcggtcgcgc gcccattcgg gatctggtcg gtgcacctca tggcggacga
    34501 gctcgccaac cacaaggtgc tcaactttac gcgtcgccac gcggacggtt gggaaggggt
    34561 caatcacgag gtgtggctcg atcatccgcc gaccgagcat gctctcgagc atgcaggttc
    34621 acacgctctt cccggctctc ctccggagtc gtgatcgggg cttcgctgga gagaaacacg
    34681 atcgagcacg gcgagtgctc cagcacttcg gcagccagct cgccgaagaa gagttgctcg
    34741 cccgagcggg ggctgacacc catcacgagc agcgtgtgac cgccgcgctc gatttcccgc
    34801 aggatggcat cccgcgtcgt ccgggcccgg cggatgacgc cgtgtacgtt gatgccatag
    34861 tgctcgccga tatcgacgat ctcacggatg gccgcgcccc ctgtattctc cggagcgagt
    34921 acgttgccga atcgctggcg ccaggcgcgc acatgacgca ccgggtttgc aacgaagagc
    34981 gccttgacgg caccgtgcga gccttgcgcg agcgcgatgg caacctcggc tgcatccctc
    35041 gacaggctcg taccgctgac cggcacgagg atgcgcaagg gatcggacag cggattacgt
    35101 cggcgcccgt gacgcgagat cgtgacggcg aatgctccgg cgaattcgac cgcgctgcgg
    35161 gtgatctgcg gctcgaaggt gttagtctgc gcgacaggct cacgtccgat gaacaacaac
    35221 ccatagcctt tgcgggcctc cgcagcgatc gtctcggccg aggcggctga ctcgccggcg
    35281 cgggtggtga tctcgaccgt cgccggcgac ttcggattgg cggactcatc tgcggccacg
    35341 gcgtctgcct tgattgcggc cgcgctctgc ttgacatggg ctcggttgtc cgcgctgcgc
    35401 tcggcgctct cgttatcgaa gtgcagaacc gtggtgggaa tgccccgtac gccggccagc
    35461 aagcccacca ggcgggaggc cagcttgccg ctcgggctgt catccaccgc cacgagcagc
    35521 cgctcgatgt gcttgacgaa gccctgcgcc tcgaactcct cgcgctcgag gcgcgcccgc
    35581 tcctccgggc tcatgggaat gcgctgcaat gcccacttca acagcggcgg catcgacatg
    35641 gtggtgatga tggccatggc gacgatggct gtgaacagac tctggttcag tgcgccgatc
    35701 gagagtccca ccgaggcaac gatgacctcg gtcgagcccc gcgcgttcat cccgcaaccg
    35761 accgccaggg actcggtgta ggtgaggccg ccgatgcgcc ccccgaggaa ggcgccggtg
    35821 aatttgccga tgctggcgag cacgatcatc ccggccgtga gcagcaacat gtccgatcgc
    35881 gcgagcgtgg atacgtccgt cgtgaggccg gccaggccga agaataccgg catgaagagc
    35941 gcgatgatca gcccgcggag ctgctcgccg atgtggcgcg tcaggatggg cgactggccg
    36001 atgagaatcc ccgcgacgaa cgcacccagt accagatgca cgccgatcgc gctggtcagc
    36061 agcgccatga ggcccgtgac cgccaggatc acggcaatga cgggaagctc gctctggatg
    36121 tgatcgttcg cccagcggat cagcatgaag accagacggc gcccgacagt gaagctcacc
    36181 accagaaaca gcgcggtgcc gagcacgctg cgcgaaaggg tcgcaaggtc gatggagccg
    36241 tgcagagcga gaccgaagat caccgacatg atgatccagc cgatcgtgtc gtcgatgatc
    36301 gccgccgcga cgatcacctg gccgacggtc cggcgcagaa agcccagctc gcgcaccacc
    36361 agggcgacga tcttgacgga ggaaatcgag agtgccgtac caagaaacag ggtggtgatc
    36421 agccgctgcc cattcgcggg caggatggaa tccggtacca gcgagccgag aatcacgcca
    36481 cagataaaag gcacgaggat gccggtgagg gaaatgctga tcgccgtccg gcgcgcatcc
    36541 cgaaacaccg acagatccgt ctccattccg gtgacgagca gcagcagcag gatgcccagc
    36601 tgagcgatgg catcgagcat cgcgtgctgg gcggcacccg gcgggaaaag gctgtgccac
    36661 agggctggac tgatcatgcc cagcaccgag ggaccgagca ggatgccgcc gatcagctgc
    36721 cccatgaccg gcggctggcc gatgcgctgc atgagctctc cgaagaatcg cccgcacacg
    36781 agcaatgtga tgatttgtgc gaggaagact gcgacggacg caccgtgcgc catgtgcgaa
    36841 actcggagcc ggaacggatc gaggcaagat agcaggttgc ttgagagccc cgcgggctct
    36901 ctgtcacccc gaccccagga tgacccgaat gcggtgaggg gtaccgtcgt cggcgagtgc
    36961 gagtccatcc gcggcgtgag ccacggcctg tccgtccagc tccactcggc tgatgccgcg
    37021 gctgacaccc ttggggtttt ccactgcgat ccgatagagg gtcgagccgt gccgatagct
    37081 catctcgaag cctcgccagg tgggcggaat gcacggctgc agcagcagtc tgcccgcctg
    37141 cagccgcagg ccgaggatcc actcgagacc cgcgcggtac atccagccgg cagagcccgt
    37201 gtaccacgtc cagccgccgc gaccctcatg cggcgggacc gaatagatat ccgcggcgac
    37261 cacgtacggc tcgaccttgt acgtctcgac atcggccggc gagcgcgcat ggtggatcgg
    37321 attcaacagc gaaaaccagt gggcggcttt cccgccatcg ccgagcatgg cctgtgcgac
    37381 cacggaccag gtcgcggcat gggtgtactg gcccccgttc tcgcggattc cgggcgggta
    37441 gcccttgatg tagccgggat cgagcggaat acgatcgaac ggcggcgtga acagcagcac
    37501 caggcccgga tcgggccgca cgagttgctc ttcgacactg gccattgcgc gccgcgcccg
    37561 agccggatca gcggcccctg acatcacact ccacgactgg gcgatcgagt ctatccggca
    37621 ctcgggactg gtggcggagc cgagcggcgt gccatcgtcg aaataaccgc gccggtacca
    37681 ttcgccatcc caggcgagct ccagcgcttt ctgcacggcc gccgcatgcg cccgccattt
    37741 ctgcgctcgg gcagtctccc cgcgtgcagc tgccaggggg gcgaacgagg tcaacgcgta
    37801 ctgcaggaac caaccgagcc agacactctc gccccgaccg gcctcgccga cgcggttcat
    37861 gccgtcattc cagtctccgg taccgaacaa cggcaggcca tgctccccta ccttcaggct
    37921 gtgatccaga gcacgggcgc aatgctcgaa cagcgaagcg ctctgcgccg agcgctccgg
    37981 ctgaaaaaac gcatcgtgct gtccggcctt gagcgtgggg ccggagagaa acggaatggg
    38041 ctcatcgagc acggccacat cgcccgtcgt ctcgatgtag tggtgcgcgc aataggcgag
    38101 ccagaccgtg tcatcggcaa tgcgagtccg cacaccttgg ccgaggggca cgagccacca
    38161 atgctgcagg tcgccttcgg gaaactgtcg tcccgctgcg cgcagcagat gctcgcgagt
    38221 gagatccggc cgcgtgacga tgaccgccat gctgtcctga agctggtcgc ggaaaccgta
    38281 ggcgccgctc gcttgataga aagccgtgcg cgcccagatc cggcaggcga tggcctgata
    38341 cagcatccac cggttgagga tgatatccag cgcccgatcg ggcgttttga cctgcacggt
    38401 gccgagcagc tcatcccact ggctcaccac ctgttggaag agcgactcca gatcggtctt
    38461 tcgaactcga gtcagcaggg tgcgcgcggc ggaagcatcc tccgcgtccc cgaggaagaa
    38521 taccgtctcc acggtttcgc ccggccgaag caggcaggag gtttgcagcg cgcagcatgg
    38581 atcgagaccg gcgcccacgc gtcccgaaag ttccgctcgg cccgcgagcg ccgcgggtct
    38641 gcgcagcgtt ccgtggcggc cgagaaactc gcttcggtcg gcgctccacc ggagctgtct
    38701 gccggcaaga tctgcgaagg cgacccgcga gccgaaggtc ccatcccagg ggttgcgggc
    38761 aaacagcgcg ccggtgtccg aatcgagatc ggtcagaatg aacggcgccg cagcgccgcg
    38821 tgaggagccg agcacccact ccgcataggc ggtcacggag agcttgcgtg cgcgctgcga
    38881 gtcgttgcgg atcctgagcc gggagatctt gaccggatcg gccatgctga caaacatcag
    38941 cagatccacc gcaaggtcct cgaccgagtg ctcgaaacga ctgtagccct gtccgtgccg
    39001 ggcggtgtac gtggcggcgg catgtcccgc aggcgatgcg gtggcgctcc agagctcacc
    39061 gctttcctca tcgcgcagat acaacacttc accaggccga tcgacgaccg gatcgttcga
    39121 ccagggggtg agcgtatgct cgcggctgtt gcgcgaccag gtgaagccgc cgccatcggc
    39181 gctcacctga aagccgaaat gcggattggt gaccacgttc acccaggggg ccggggtcgc
    39241 ttccccgggc gcgaggatcg tcacgtattc gcggccgtcg gccgcgaatc ccccgagacc
    39301 gttgaagaat tcgagatcgc tctgctgcgg gggttgggcg cgttgcgccg gtggccttct
    39361 ttgagacagc cccccatctt gagctcgcgg cacaccgctc ggcgcccgtg cagaggccct
    39421 gccgagctgc tccgcgaggc tcccacgccc gggatcgatc acgactcgcg ccgctgatcg
    39481 gagcagcgcg cacgtctgcg cggggatctt gtcggcgcca agggagatca ccgagccgcg
    39541 aattccagtg ccctcggctg gatcatgctt cggccttcct tccatttccg cctttactgc
    39601 ggctggcagc tgcgtgccgg gcgagctctg cgtgctcagg atgacgagat ccacgcccag
    39661 accccgcgct cgccagtact catgagcctg cagaaactgg cgcagcatcg ggagctgctc
    39721 gatcgcatcg agctgcagca gcaacaacgg cagatctccg gagatgccct gcgcccagag
    39781 cgcgcccggg gggccgctgc ctgcgcggat gacttctggg ggtgcgcgca ttgcgccgtt
    39841 tgcgtacagc agatggccag ccagtcgtcg aaacagagct gccgtttccg gcgccagctg
    39901 ccgctcttgc agatctctct gcgcctgttt gcgcgctccc tcatccgcgc gcgcgaaaga
    39961 gcctgcttcc cgctgtcgct tgaccaggtt gagcacctca tcgcgcgaag ctgcaacact
    40021 cgtccaataa cacagtcgcg cggtctcgcc ggcgccaacc cgaatgcggt agcgaagcgc
    40081 aaacaccgcg tcgatgacgg tgccgacggt gttggagagc ggcctcccat ccacgatggc
    40141 gctcgcctcg ccgacctcgt gtccgcgccc caaaaaccgg gcgcggtcgg tctccacttc
    40201 cggctcgctc agcagcgcgc cttccacggt ggcgtgatga gcggcccaga tatgaggctc
    40261 gtccggggaa cggttgcgtc gggtggccag gatggcatcg gggtagcgta cgcgctcggt
    40321 ctgcacgaag agcttgagaa aggccgggtg gccggcatcg gccgcgggcg gcgccaacat
    40381 cagctcggcg taggaggtga tctccagctc gcggctgcga tcgccgctgt tggtgatcga
    40441 caggcgccgg gcctcggcat cgtgctcggg tgagacgagg atttcgaggg tggtcgagag
    40501 cccgtgatcc gggcgggaga tttccgccct ggactcgctg aaggtcaccg taccgccgcc
    40561 cgctgcgctc ccgcagggtt gctcggttgc cgaccacacc gcgccgttgt gcagatcacg
    40621 caggaaaaaa taggcgcccc agtcatcgcg cgtcgtatct tcccgccagc gtgtcactcc
    40681 gagatcgcgc cagcggctgt agccggagcc ggcgcccgtg agcatcaggt gatagcggcc
    40741 attggagagc agatgcacct gcggagttgc ttcgcctgcg ctgacttcgt tcaccacttg
    40801 cctgcctccg cgctgggttc tccatcttag ccgcgagggg tcctcacgca acagccacgc
    40861 tccggtttgc tcagcgcgag cgcacgaatc ggaatgcgtg cagcgcccat ccagggcatc
    40921 ctcgggccgc tatggggaag aaagaacacc ggctcgcgga gatcgattgg cagcggatcg
    40981 gcaccgagct ggacagctgc ggcgcggcga gaatcgacgg cctgctcggc gcgagcgagt
    41041 gccgcgcgct ggcagcgctt tatgccgatg agcggcactt tcgcagccgg gtggtgatga
    41101 gccgccacgg cttcggacgc ggcgagtacc agtactttcg gtatccgttg ccgccgctca
    41161 tcgcggagct gcgcgcgcag ctgtacccct cgctcgctcc gatcgccaac cgctggaatg
    41221 agaagatggc gatcggttcg cggtatccgg cgacactcga gcccttcctg gcgcgttgcc
    41281 acaaagcagg tcagccgcgg ccgacgccgc tcctgctgcg ttatcagccg ggtgattaca
    41341 actgcctgca ccaggacctg tacggcgagc attgctttcc gctgcagctg accattctgc
    41401 tgtcggagcc cggcaaggat ttcacgggtg cagagttcgt gttgacggag cagcggccgc
    41461 gcatgcaatc gcgcgtggaa gtcattgcgc ttcagcgcgg cgatgcggtg atcttcgccg
    41521 tcaaccagcg gccggtccaa ggtgtgagag ggatctaccg ggtcaaacac cgccatggcg
    41581 tgagccgagt ccgaagcggc gagcggtatt cagccggcgt gattttccat gacgcgaaat
    41641 gagagctccg gcggctcgct caagccgcca gcagagcgac ctcgacgcga tttcgacccg
    41701 atcgcttcgc gacatacacc gcggcatcgg cacgcgcgat gagattttcg ccccaggctc
    41761 cgggtgagtc gctcgtcgcc actccgatgc tgcaggtcag actgattggc tcaccgaatc
    41821 cctcaacgcg cacgccagcc actcgcgcga gaatccgctc ggcgatcgga tacgcggccg
    41881 cggcatccgc gctgctcagg atcacgacga actcttcacc gccgtagcgg ccgatgacat
    41941 cggactgacg cagctcggcc tggatgggcg cgatgatcga ggtcagacag gcatcgccgg
    42001 ctgcatggcc gcgcgaatca ttgatctgct tgaagtgatc gagatcgatg aagagcagcg
    42061 agatgggaag accgcgtgcg cgggcgcgca ggcaggcggc atcgaggcgc tcgaggaacg
    42121 agcgccggtt gaggacaccg gtcaaggagt cggtttgtgc gcgccgctcg gcctcgctga
    42181 gagcgaggcg ctgctcccgc agtctgtctg cgagccccag ggcgaccagg agggcggcgg
    42241 cgaccatgga caagggcagg ccgacgtaca gtagacggcc atcgtcatca cctttgagca
    42301 gcagtgttgc ggtcgtcgcg atcgtgaagg cctccagcag cgcccaggcc agaaagaacc
    42361 agcccgctgc ccggctcccg cgacgccacg ccacaaaaag cacgatcagc gtgaacgtcg
    42421 cgctggcgag aaacagtgca ttgccggcgg cattgacgat cagaccccac ccgatgagct
    42481 tcagcaggtt ggcgaacgtc agggccacga atgcccaggc gagccagcca aagatggtgt
    42541 aaacgcgagg cgagaagcgc cggagatcgg ctagctcacg cgtgaacaga cacgctgccg
    42601 cgccgccgag tgccaccggt acgttccacg catgcgagct cagcggcagc gcgaaggaga
    42661 gcaggggcca ttcgaagccc tgtcccgaca gatagacgac atagagcgct tgcagcgaga
    42721 agagcgccgc gtagaggatg aacagctttt cacccagcac cagccagatg agcagcgccg
    42781 cgatcgacat cgccatgagc gcgccgaagg cgagcgcgat catccgcgca cgggtggtcc
    42841 cggctctgag cacctcctcg agcgaggcga gagagatgcg cggcagctcg gatccgcgca
    42901 ccgcggggac tatgcgggca tacagtgcct ggcgtccgga agccgcggcg gccggcagca
    42961 cgaagaccac ttcctgtgca ccgccaaacc ccggtaaccg ggccgctcgc ggcagctccg
    43021 agcccacatc ctggccgtca ccggtgccat cggtgcggct gctggccgca gcatagaccc
    43081 gaacttgcgt gagccggcct gtgtgaacag tcagtaccgg gaccgagccg ccgtctatgg
    43141 ccgcggcagg ctggagccgc agccagaaag gcgtgcctcg cgccgccagc tcagcgtacc
    43201 ctgcggaagt gaaccctgag tcgagggatc ctctgactac ctgtgccgcg ggcggatccc
    43261 cagaatgagt agcgagcacg cggacgtcga gcgtggcggc cgctagagcg ctgctgaatg
    43321 caagacacag cacgaacacc tgggcctgcc tcgcgccgcg ttgcgacgcg cgcccgggac
    43381 gtagactgct catcggaggc aacaccgcga actgcgcagt cagggattat gccgcaaacc
    43441 gggtcgcggt cgcccggctg gaagatcaac gtcgctccca gtgaccccgt gcgagatgcc
    43501 atccatcacg ctgatgcacc cagtgatggg aaacccagcg ctcatgcgga tgcgggcgct
    43561 cccagtgtcc cgaaacccac tcgtgacggc ctccgaccca gttccagtac ccacccagcc
    43621 agatgtagcc agggtacggc ggagcaccgt attcctcgac gcgcggtgga ggcggcgcga
    43681 cgtccaccgg gcccccgaca tagtattcat tgggaggacc ggcagcgacg atgcatccgc
    43741 cgagcgagat tcccagcaca aaaattgcta cgagtttcgc gacgcgaaat ccagcctgac
    43801 gcatatgcac tcccgacggt cgccacactg gggcgccatt cgtttgaacc cttttggcac
    43861 taacgcaaga gaccgcgatt ggttcacaac acaggacgaa tcccatgccg gatgcccgtg
    43921 aagacatcgt attcctcttt gacgtagata acacgttgct ggacaacgat gccgtgcagg
    43981 cggatctcgg cgaccatctg gagagcgagt tcgggcgtgc cgcacgcgag cgctactggg
    44041 cgttgttcga gacactcagg tccgagctcg gttacgccga ttacctgggt gccctgcagc
    44101 ggtatcggga agaggatccg gatgacatgc agctgctgcg gatgtccgcc tttctcatcg
    44161 actacccctt tgccagccgg ctgtaccctg atgcgctgag cacgctggcg cactgcggcc
    44221 gcagcgggcc ggtcgcgttg ctgtccgatg gcgatgtggt cttccagccg cgcaaggtcg
    44281 agcgctcagg tctctgggct gcggtcgagg accgcgtgat gatctacctg cacaaagagc
    44341 agatgctcga ctccgtcgag cgacgttatc cggcgcgaca ctacgtgatg gtggacgata
    44401 aactgcggat cctgacggca atgaagaaga tctggggcac gaagctcacc accgtgttcg
    44461 tgcgtcaggg acattacgca ctggacatag ccaacatcag tatctacccg cccgcggacg
    44521 tgaccctcga gtgcatcgcg gatctccgcg cccacaccct cgccgagctg tctggagcaa
    44581 catgagcagc ccccaagtga atcccctggc gggcaagccc gccacgcccg acatgctgac
    44641 cgacgtgccg cgcctggtca ccgcctactt cgctctgcag ccggatgtcg ccatcccggc
    44701 tcagcgcgtc gcattcggaa cctccgggca ccgcggcagc gccctggagc gaggcttcaa
    44761 cgagtggcat gtgctcgcca tcagtcaggg gatctgcgaa taccgccgcg ggcgcggcat
    44821 cgatggtcct ctgtatctcg gcatcgacac tcacgcgctg tcggttcctg caagcgtcag
    44881 tgcgctggaa gttctggcgg cgaacggcgt cgaggtcatg ctgtccgagc gcgacgaata
    44941 caccccgaca ccggcgctct cgcacgcgat actcacctac aatcacgggc ggcagagcgg
    45001 gctggccgac ggcattgtgg tcacgccttc gcacaatccc ccgccggatg gcggcttcaa
    45061 gtacaacccg ccgaacggcg gaccggccga caaggacgtg acaggctgga tcgagactcg
    45121 cgcaaacgag ctcctcgggg ccggcctgca gggtgtgaag cggatcggtt acgagcaggc
    45181 gctgcgggcc gcaaccaccc accgctacga tttcctcaac cgctatgtcg atgacctcgc
    45241 gcaggtgatt gacctggatc cgatccgggc ctcgcaagtg aacgttggcg tcgatccgtt
    45301 gggtggcgcc ggcgtgcact actgggctgc gattgcggac cgatatcgtc ttaagctgaa
    45361 cgtgctcaac actgcggtcg acccgacgtt ccgcttcatg acggtggact gggacggccg
    45421 catccgcatg gatccgtcct cgccctatgc catgcagagc ctcatccatc tcaaggaccg
    45481 ctacgatatc gccttcgcct gcgataccga ccatgaccgc cacggcatcg tgacgcgcag
    45541 cgccggcctg ctgccgccga atcactatct cgccgtctcg atcgactatc tgttccggaa
    45601 ccggccgcga tggcccgcgg cagcgggagt gggcaagact gtcgtgagca gcggcatgat
    45661 cgatcgggtc acgagccggc tcgggcggcg gctgtacgaa gtaccggtcg gattcaagtg
    45721 gttcgtgagc ggcctcagcg atggctcgct cggcttcggt ggcgaggaga gcgcgggtgc
    45781 ctccttcatc tgcctggcca gtggtccctg gagcaccgac aaggacggca tcattccctg
    45841 cctgctcgca gcggagatga ccgcgcgcaa gggccgagac cccggcgatc tgtatcgtaa
    45901 tctgacgcgg gagtttggcg agccggcgtt cgagcgcgtc gatgcgccgg cgacccgcga
    45961 gcagaagcag atcctgaagc gcctctcgcc ggcccaggt
//
LOCUS       DASRHN010000149         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_541779, whole genome shotgun sequence.
ACCESSION   DASRHN010000149 DASRHN010000000
VERSION     DASRHN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541779"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VFB37_05770"
     CDS             <1..1390
                     /locus_tag="VFB37_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO21995.1"
                     /translation="GQQATATLVFAAMSLTVIATLAGRGPFLTWDIGASLMQVALFMA
                     VVAVTGLFLGAATAERNRAERRRTEDYARLRVSEERLARQADELAAADRRKDEFLAVL
                     GHELRNPLAPLQNGLELLSLGGTDPALVTHARQLMERQLRHLVRLVDDLLDVARIRSG
                     KIVLELERLEIADLVASAVELARPLIDARRHELEVVLPDEPLWADADHIRLPQLLANL
                     LNNAAKYTDEEGRIILAVARLEDDIVITVRDTGIGMTAEALAEVFELFTQAVGPAHAV
                     QGGLGVGLSLARSIAHMHGGRLTAHSAGPGKGSEFVLRLAAAPPPATLTTAPTADRPP
                     ELRQRILVVDDNIDAAETLATMLAYSGHDVRVANGGREALAAAREFAPGVMILDLGMP
                     EMDGYAVARAVRADAQLASILLIALSGYGQPDDRRRTAAAGFDAHLVKPVEHEVLNAT
                     LGESSARRLRTG"
     gene            1538..2683
                     /locus_tag="VFB37_05775"
     CDS             1538..2683
                     /locus_tag="VFB37_05775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO21996.1"
                     /translation="MHLVDTTIFYSPTSGGVKRYLNAKHAWLAAHTPWEHTIVVPGSE
                     DRLDRGGVCTLSGFRVPRTFNYRLPINPRRWSQLLDSLEPSLIEAGDAFHPAWCAAHV
                     AQRRGIALAAFYHSNLPQIIGRRVGSLSERVLSRYVRALYERFDVVFAPSRLMCAFLK
                     ELGIQHAIHQPLGVDTAVFRPDCRRPDLRERLGLPRDTRVLVYAGRFAAEKNLPVLFR
                     AIDLLGRPYHLLMIGGERAARPASNITLLPYHRDSAELAEWLASADALVHAGTKETFG
                     LVILEAMACGRPVVATRAGAIPEFVDGNVGMLAEPNSAHSMAEAIAGLYERDLDRLGA
                     AARERVMQRFTWNQAFHLQINAYASLLGARRPALPAREAIELGPSSS"
     gene            complement(2716..3162)
                     /locus_tag="VFB37_05780"
     CDS             complement(2716..3162)
                     /locus_tag="VFB37_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZO21997.1"
                     /translation="MQLTLYALAFTAGLVLTVQVGVNATLRTSLGNPVMAALTSFLVG
                     LVGLALYLVLTRASLPTKAALSAVPLWAWAGGLLGAFYVASTIIVGPRLGAAALLSLS
                     ILGQLLASLVVDNFGWLGFPSHPISVVRVIGAALLFVGVLLIVRVG"
     gene            complement(3214..>3517)
                     /locus_tag="VFB37_05785"
     CDS             complement(3214..>3517)
                     /locus_tag="VFB37_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO21998.1"
                     /translation="PEILHELVVEQMPGHDSYIEAGNGEAGPRRELKGFRLHSAEGEW
                     VFRALAYHLHREVSESFYRAIPPRSAPWSKVLFWRIVLALAARPAGKRLLLALRRH"
BASE COUNT          594 a         1188 c         1159 g          576 t
ORIGIN      
        1 cgggcagcag gccacggcga ccctggtttt cgccgcgatg tcgctcaccg tcatcgccac
       61 gctcgccggc cgcggaccgt tcctgacctg ggacatcggc gcgagcctga tgcaggtggc
      121 cctgttcatg gcggtcgtgg cggtcaccgg attgttcctg ggcgctgcga cggccgagcg
      181 caatcgcgcc gagcggcgcc gcaccgaaga ctatgcgcgg ctgcgtgtca gcgaagagcg
      241 gctggcgcgt caggcagatg agcttgccgc ggccgatcgc cgcaaggatg aattcctcgc
      301 cgtccttggc cacgagttgc gcaatccgct cgcaccgctg cagaacggac tcgagctcct
      361 ctccctcggc ggtaccgacc cggcactcgt cacgcatgcg cgacagctca tggaacgaca
      421 gcttcgccac ctggtacggc tggtggatga cctgctggat gtcgcacgca tccgctccgg
      481 caagatcgtc ctcgagctcg agcgcctcga gatcgccgac ctggtcgcca gtgcggtcga
      541 gctcgcgcga cctctcatcg atgcacgacg gcatgagctc gaggtggtcc tgccggatga
      601 gccgctgtgg gccgatgcgg atcacatccg gctgccgcaa ttgctcgcca atctgctcaa
      661 caacgccgcg aagtacaccg acgaagaggg ccgcatcatc ctggcagtcg cacggttgga
      721 ggatgacatc gtcatcaccg tgcgggacac cgggatcggg atgacagccg aggccctggc
      781 ggaagttttc gagctgttca cgcaggccgt cggacctgcg catgccgttc agggcggatt
      841 gggggtcggc ctgagtctgg cgcgcagcat tgcccacatg cacggcggcc ggctcaccgc
      901 acacagcgcc ggaccgggca aggggagcga gttcgtgtta cgcctcgcgg cggctccgcc
      961 gcccgcgacc ctcacgacgg ctcccacggc cgacaggccg cccgagctgc gccagcgcat
     1021 cctggtggtg gacgacaaca tcgatgcggc ggagacactc gccacgatgc tcgcgtattc
     1081 cggtcatgac gtgcgtgttg cgaacggagg acgcgaagcg ctcgcagctg cgcgggagtt
     1141 cgctccggga gtcatgatcc tcgacctggg gatgccggaa atggatggtt acgcagtggc
     1201 gcgggcggtt cgcgccgacg cgcaactcgc ctcgattctt ctcattgcgc tgtccggtta
     1261 cggacagccc gatgaccggc ggcgaaccgc cgccgccggc ttcgatgccc acctcgtcaa
     1321 gccggtcgaa cacgaagtgc tgaatgccac attgggcgag tcgagcgcgc gccgactgcg
     1381 gactggttga ccaccggcgt cagcctcgga tgttggtcgc gccgtgcgtc gcggtccatg
     1441 cgcgtgcccc ggcttgacag tcacgcgggc ggagggtgca atcgggcccg ccgcttgctg
     1501 gcaggcaggg gtcgctagac gcacggtgaa cgaaagaatg catctcgtcg atacgaccat
     1561 tttctactca cccacgagcg gtggcgtcaa acgatacctg aacgccaagc atgcctggtt
     1621 ggctgcccac accccgtggg agcatacgat cgtcgtgccc gggtcagagg atcggctcga
     1681 ccgcggcggt gtgtgcacac tgagcgggtt tcgggtcccg cgcacgttca actaccgcct
     1741 gccgatcaat ccacggcgct ggtcgcagct gctggattca ctcgagccga gcctcatcga
     1801 ggccggcgat gcattccacc cggcgtggtg cgcagcgcac gtggcgcagc ggcgcgggat
     1861 tgcgctcgcg gccttctatc attccaatct gccgcagatc atcggtcgcc gggtaggcag
     1921 tctcagcgag cgcgtcctga gccgctacgt gcgcgccctc tacgagcggt tcgatgtggt
     1981 gttcgcgccc agccggctga tgtgtgcctt cctcaaggag ctgggtatcc aacacgccat
     2041 tcaccagcct cttggcgtgg acaccgcagt cttcaggccc gactgccgcc ggcccgacct
     2101 gcgtgagcgt ctcggtctgc cgcgcgatac gcgggtgctc gtgtacgcgg gacgatttgc
     2161 agccgagaag aatctccccg tgttgttccg cgcgatcgat ctcctcggcc ggccgtatca
     2221 tctgctcatg atcggcggcg agcgcgcggc gcgcccggct tccaacatca ccctgctgcc
     2281 gtatcatcgc gacagcgcgg agctcgccga atggcttgcc tcggccgatg cgctggttca
     2341 tgcgggcacc aaggagactt tcggtctggt gattctcgag gcgatggcct gcggccgccc
     2401 ggtcgtcgcc acccgcgcgg gcgccatccc ggagttcgtg gatggcaacg tcggaatgct
     2461 tgccgagccc aacagcgcac acagcatggc ggaggcgatc gccgggctgt atgagcgcga
     2521 tctggatcgg ctcggcgcgg cggcccggga gcgtgtcatg cagcggttca cgtggaacca
     2581 ggcgttccac ctgcagatca acgcgtacgc ctcgctcctc ggcgcacgcc gccccgctct
     2641 gcctgcgcgc gaggcgatcg agctcggtcc ttccagctcg taatctgcaa ctcgcgaccg
     2701 cggctgtgcg ttggcctaac ccacgcgcac gatgagcagg acgcccacga ataacagggc
     2761 tgcaccgatc acacgcacca ccgagatcgg gtgtgacgga aagcccagcc agccgaagtt
     2821 atctacgacc agcgaggcga gcagctggcc gagaatactc aaggagagca gggcggcggc
     2881 ccccaggcga ggaccgacga tgatggtgct cgcgacatag aacgcgccca ggagcccgcc
     2941 tgcccaggcc cacagcggga ccgcggaaag cgccgctttc gtcggcaggc tggcacgagt
     3001 caggaccaga tacagcgcaa gccccaccag gccgacgaga aagctcgtga gcgccgccat
     3061 cacgggattt ccgagtgacg tgcgcaaggt ggcgttcacg ccgacttgca ccgtgaggac
     3121 cagtcccgca gtgaatgcga gtgcatacag cgtgagttgc atgatcatcg acctcgatgg
     3181 tgacgcgcga cccgcgagag tgccgtccgc cgctcaatgt ctgcgcaacg ccagaagcag
     3241 ccgctttccc gcaggccgcg ctgccaatgc cagcacgatt cgccagaaca acaccttgct
     3301 ccagggcgcg gagcggggcg gaatcgcccg atagaacgat tcggagactt cccggtgcag
     3361 gtggtacgca agggcgcgaa acacccattc gccctcggca ctatgcagcc ggaaaccctt
     3421 gagttcccgc ctcgggccgg cctcgccatt cccggcttcg atgtatgaat cgtggccggg
     3481 catctgctcg accaccagct cgtgcaggat ttcggga
//
LOCUS       DASRHN010000150        11384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1532396, whole genome shotgun sequence.
ACCESSION   DASRHN010000150 DASRHN010000000
VERSION     DASRHN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532396"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFB37_05790"
     CDS             <1..455
                     /locus_tag="VFB37_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZO21999.1"
                     /translation="ATRGRTPGKRMAGVELVARDGGAPGVGPLLTRNVFRLIDSLPLF
                     YAVGLTTTMMTRDHLRVGDLAAGTLLVYVRSNAMLPAYLVGRPAASALDARSAEIVTE
                     LLERWPGLDTAARHRLAKALLPPSESTEAEDDAVLRMRLEKLAAGTSL"
     gene            452..1459
                     /locus_tag="VFB37_05795"
     CDS             452..1459
                     /locus_tag="VFB37_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZO22000.1"
                     /translation="MNDSARDHTLRLTLLQRSALWKAAAERTRRLARGRSNDPLDAAR
                     MADDYRLLAHDVARARRLLPNTRTLDYLESAYAQAHTTLHRAAWHPGYALLHLFRDEI
                     PDVVTWLWPHILWATTLFLLTVGAGYWLVHIYPDLVALFASPDLIASVERGQLWTEGL
                     LNIVPSSVLSVQILTNNIVVSLCAYCAGFLFGLGTLYILGLNGLMLGAVFAFTSLHGL
                     GRELFGFVVAHGCVEISVMCLSGAAGAAVGEALVRPGAATRAESFRLAALRSGKLILA
                     CVLLLIGSGVIEGYVSPNPDIPLGPRILIGLSYWLLMVALLRGWLFGRLRAHRPAIAG
                     G"
     gene            complement(1462..2760)
                     /locus_tag="VFB37_05800"
     CDS             complement(1462..2760)
                     /locus_tag="VFB37_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZO22001.1"
                     /translation="MHLAQRAFVLILLTAVLAIAGIWSSEPGLSEAWRLPAALLLLGL
                     AYEAYRIRRASVRADVETAARAFLGRQQAGAFAFRNETLRSVRMQYAPAAPAGIRTEM
                     PVRTIVAEAGQIKRDPFVLLPVRLGRQVWPGLPARLLGPLGLAWWSRVLYPSAQLAVA
                     PDTLNTIRGRPRGNPAGARPRRVIGAGSELHQLRGYVRGDPPARIDWKATARAHKLVT
                     REFSEDQHLDVLVAIDAGRLSRVRAGRLDRFGLYANVAARLAEAVVPNDDRIGLVVFA
                     DQPLTVCPPGRGLSAVMRIRKTLESLVVQGAESDPLAAAVRIRGMLQHRSLIVMLTDL
                     DDASVADQLTRAVRLLSPPHLVVVAGVQSREIGELARAPARNWQDPWIALAAQEHEDR
                     AESQRLLLRRLGAPVIAAREALLERAVLLEYETLRRTRRV"
     gene            complement(2771..3721)
                     /locus_tag="VFB37_05805"
     CDS             complement(2771..3721)
                     /locus_tag="VFB37_05805"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZO22002.1"
                     /translation="MSSSLQELEVFLRQELGRVIVGADASIRALVIALVARGHVLVQG
                     VPGLGKTLLAKALARALGGEFKRIQGTPDLMPSDIIGVHVFEDERRAFVFRPGPLFAD
                     VVLVDEVNRAGPKTQSALLEAMEERQVSVEREVLALPEDFLVLATQNPREFEGTYPLP
                     ESQLDRFMLRIDVSYPERIAETEILARYGKTLTASRDALQEVGTLPAALLEQARMEVD
                     RVHVSEAITGYVLDIAQASRTQARLSLGLSTRGVIALVKAARTTAALRGSEFVAPDDV
                     KEVAAWVIAHRLVLTPESALEGMTDLQVVDRLLAETPAPR"
     gene            complement(3718..5007)
                     /locus_tag="VFB37_05810"
     CDS             complement(3718..5007)
                     /locus_tag="VFB37_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZO22003.1"
                     /translation="MRERFTTLLCALGALLLFLTMFVHGRAVTGEPEVARPTTAERGG
                     NGYFAVLQWLRDAHIRAVSLRNRFDELAARHDLPAAGNILIVTLPAIAPYKTEEFRPL
                     DRWVRAGNTLLVLAALSDSPDWAFSIGNLTTGDLNLLTGLDFETVKARDRRSEAARSG
                     QPSRAPRQAERGTLSDRIVAATRKLTEPDRETFVANRPHAYFTGVREGLALSDYASQA
                     WSVQVPYDGFVLELAHAQATGEGVLWTRSLGSGRIIVSALGSLFTNRSIGLEDNARLL
                     ANIVGANIGSDGAVLFDDAHQGLGAAYDPAKFYRDPRLRLTIGILAGLWLCWVLGATR
                     LRLPTLRLPAPREEELVRATGGFLARVLTPAAAARRMIDRFLQRSPWEALERHPQIAA
                     ADIQQLKSWYADAYVSDRVPLVRVHDLICKINRQLHS"
     gene            complement(5004..5996)
                     /locus_tag="VFB37_05815"
     CDS             complement(5004..5996)
                     /locus_tag="VFB37_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22004.1"
                     /translation="MTLGRTAALFLSLACAAWASAPVNAQVAPAPAATPQSAPPSSAL
                     ASLDECISRLDAQTDIGYERIAARCPDLTRQLEQSEWSRWLPRGWREPRNDLSAASLR
                     ELHDLASRELATRITERTPHVQLLTGVLAQLGETRAGQVTSWMRVKRWLRSIFDSREQ
                     TDNEGWLSRLISRIGFSQSMLDLLAYAALAAVVTLAVLIVLNEFRMAGMLEKRRRQAH
                     RRSAHEGTISSQTGWEVVEGASVGDRPRVLLELIVTRLTGPPFVAPSRALTVRELVRA
                     AAVPAAEDRHRLTALASAAERVRFSGHAIPTDALEVPLVRGRELLERLSAQAPA"
     gene            complement(6007..6822)
                     /locus_tag="VFB37_05820"
     CDS             complement(6007..6822)
                     /locus_tag="VFB37_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22005.1"
                     /translation="MYPPSRPQSIGEVLDAAFRIYRVTLLGCLPFGVLAIVAGQLPNI
                     YLIARHRPLRQFGGGDPWWFVLYGVGVFFSIAFVNVIVLRQGAALRGSLSAKGALAAG
                     VRKAPVTFMAGILLFLAVGACFIPLAVVRPAYVKWGFVLLCVPATYVGVVLSCCIIAV
                     LMGDKGVFASLRYSAHLVRGNWWRVSTIFSVAIAMLAVFYVLLGVLAALLTPLLGIAD
                     VAVFAAVSAVMAAVLGAVGTSFYSAIALAMYGDLEARREGTDLQRRIASAPVN"
     gene            6853..7707
                     /gene="prmC"
                     /locus_tag="VFB37_05825"
     CDS             6853..7707
                     /gene="prmC"
                     /locus_tag="VFB37_05825"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZO22006.1"
                     /translation="MDGNLTVRALLAQAIEDLRGASGAPASVPLDAELLLGHALGMSR
                     AQIRSHPETVPTDERAQRYRRLVQRRKLGEPVAYITGQREFWSLPLAVSPAVLVPRPE
                     TELLVERALSLHPQPTARVLELGTGSGAIALALASERPAWLITATDVSEEALVAARTN
                     AATHGLQRVEFLRGSWFAPLAGRVFELVVSNPPYVAEADSALQSPALTYEPRLALAGG
                     VDGLLCLRAIIREAPQYLERRGWLLLEHGAEQADAVARELVVRGFGHVRSHRDLAGHE
                     RMTEAQWL"
     gene            7737..8228
                     /locus_tag="VFB37_05830"
     CDS             7737..8228
                     /locus_tag="VFB37_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO22007.1"
                     /translation="MIRFETSHGNFTVELFQKEAPLSTANFQKYVDDGHFDGTIFHRV
                     IPGFMIQGGGLTGDFASKSTREPIKNEAKNGLKNKRGTLSMARTSDINSATAQFFINL
                     VDNGFLDNSARDYGYAVFGRVTEGMDTVDKIAAVKTGRRKGYQDAPLEDVVIVSARTV
                     EGT"
     gene            8215..9006
                     /gene="mtgA"
                     /locus_tag="VFB37_05835"
     CDS             8215..9006
                     /gene="mtgA"
                     /locus_tag="VFB37_05835"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZO22008.1"
                     /translation="MKGPKQSRGSVTGWLLKWAIFLLIAFLLCSTLLVLMFRWLNPPT
                     SAFMVEARVQAMREGDRHYRTDYEWASLEHISPNAALAVVASEDQQFPFHAGFDLHSI
                     REAVRASDRGHRLRGASTISQQVAKNLFLWNGHSFVRKGFEACLTVLIEAIWPKERIL
                     EIYLNIAEFGRGIYGVEAAAQRFYHKPAATLTASEAASLAAVLPNPLRMHPDRPSAYV
                     LSRREWIMSQMRDLGGPAYLQAVEKELQPAAHHPSMRAGEPLRAR"
     gene            complement(9023..>11384)
                     /gene="metH"
                     /locus_tag="VFB37_05840"
     CDS             complement(9023..>11384)
                     /gene="metH"
                     /locus_tag="VFB37_05840"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZO22009.1"
                     /translation="AGLKCVQGKAVVNSISMKEGEEAFIEHARKVRRYGAAMVVMAFD
                     EQGQADTVERKVAICKRCYDILTTRAGVLPEDIIFDPNIFAIATGIEEHNGYGLAFIE
                     ATRQIKAALPDVLVSGGVSNVSFSFRGNDPVREAMHSVFLYHAIAAGMDMGIVNAGQL
                     AIYEEIAPDLREAVEDVVLNRRPDGTERLLEIAARFKGEGGAKRQADTQWRSLPVAKR
                     LEHALVKGIDDFIIEDTEEARLTFERPLQVIEGPLMDGMNVVGDLFGSGKMFLPQVVK
                     SARVMKRAVAHLIPFIEKSQTGAMRSNGRIVVATVKGDVHDIGKNIVGVVLQCNNFEV
                     IDLGVMVPCEKILDAARRENADYIGLSGLITPSLDEMVNVAKEMQRQGFEIPLLIGGA
                     TTSPAHTSVKIAPQYKSPVIYVKDASRSVGVCQTLSTETSRADFLEKIAAEHERRREQ
                     HAGKKTKAPEVSLAQARANRRRIDWTAYAAAAPRVPGIQVFDSYPLKDLLGYVDWMPF
                     FNAWEFAGKFPDVLTDPKVGEAASNLYDDARRMLKELIAESWLTARAVIGLFPANSVG
                     DDVEVYVDESRSEVLTRLSFLRQQKGKAPGQPHECLADYVAPRSSGVADHIGAFAVTA
                     GVGIEEHLARFERGHDDYSSIMLKALADRLAEACAEHFHERVRRQHWGYAARESLTNA
                     QLIREEYLGIRPAPGYPACPDHTEKAKLWKLLDPERNVGIRLTESFAMYPTAAVSGWY
                     IGHPEAHYFHIGKIDRDQVEDYARRKEMTVQELERWLAPNLGYEPR"
BASE COUNT         2027 a         3889 c         3525 g         1943 t
ORIGIN      
        1 tggccacccg cggccgcaca cccggcaagc gcatggccgg tgtggagctt gtggctcggg
       61 acggcggtgc ccccggagtg gggccattgt taacccgcaa tgtctttcgc ctgatcgaca
      121 gcctgcccct gttctatgcc gtgggcctca ccacgacgat gatgacgcgc gatcacctgc
      181 gagtcgggga tctcgccgcc ggcacacttc tggtgtacgt gcgctcaaac gccatgttgc
      241 ccgcgtacct ggtcggccgc ccggccgcct cggcactcga tgcgaggtcg gccgagatcg
      301 tgacggaatt gctcgagcgc tggcccggat tggataccgc ggcccgtcat cgcctcgcga
      361 aagcattgct tccgccttct gaatcgaccg aagctgagga cgatgcggtg ctacggatgc
      421 gccttgagaa attagctgcc ggaacttccc tgtgaacgac tcagctcgcg accacacgct
      481 gcgactcaca ttgctgcagc gctccgcact ctggaaagcg gcggccgagc gcacccgccg
      541 gctggcgcgt ggacgaagca acgaccccct cgatgccgcg aggatggccg atgactatcg
      601 tctcctcgct catgatgtcg cccgggcccg gcggctgcta cccaacacgc gaacactgga
      661 ctacctggag tccgcctacg cgcaggccca tacaacgttg catcgggccg cctggcatcc
      721 cggctatgcg ctgctgcatc tgttccgcga cgagattccg gatgtcgtca cctggctctg
      781 gccgcacatc ctctgggcca cgacactctt cctgctgacc gtcggcgcag ggtactggct
      841 ggtccatatc tatcccgatc tcgtcgcgct cttcgcctcg cccgacttga tcgcaagcgt
      901 cgagcgcgga cagctctgga ccgaaggctt gctgaacatc gtgccttctt ccgtgctgtc
      961 cgttcagatt ctgaccaaca acatcgtggt cagcctgtgc gcctattgcg ccggcttcct
     1021 attcggcctc ggcacgctgt acatcctggg cctgaatgga ctcatgctgg gagcggtgtt
     1081 cgcattcacc agcctgcacg gcctcggccg tgagctcttc gggttcgtcg tggctcacgg
     1141 ctgcgtggag atttccgtca tgtgtctctc gggcgctgcc ggcgcggcgg tgggcgaggc
     1201 gctcgtgcgg ccgggcgcgg ccacccgtgc cgagtccttc cgccttgcgg cgctacgctc
     1261 gggcaagctc atcctcgcct gcgtcctgct cctgatcggc tccggagtga tcgagggcta
     1321 cgtctcaccc aatcccgaca ttcccctcgg gccgcgcatc ctgatcggcc tcagctactg
     1381 gctcttgatg gtcgcgctgc tgcgcggctg gctgttcggt cgcttgcgtg cccaccgccc
     1441 ggcgatcgcc ggcggctgac ttcacactct cctcgtcctt ctcaacgtct catactccaa
     1501 cagcaccgcc ctctccagca gcgcctcgcg cgccgcaatc accggggctc caagccgccg
     1561 cagcagcaag cgctgcgact cggcccggtc ttcatgttcc tgcgcggcga gcgcaatcca
     1621 gggatcctgc cagttccgag ccggcgcgcg cgccagctca ccgatttccc gactctggac
     1681 tccggccacc acgaccaagt gcggcgggga cagcaatcgc accgcgcgcg tcagctgatc
     1741 ggccacgctc gcatcgtcga ggtcggtcaa catcacgatc aggctgcgat gctgcagcat
     1801 tccccgaatc cgtacggctg cggccagggg atccgactcc gccccctgca ccacgaggga
     1861 ctccagagtc ttgcggatgc gcatgacggc tgaaaggcct cgccccggcg ggcacaccgt
     1921 gagcggctga tcggcaaaca ccacgaggcc aatgcgatcg tcgttgggca ccacagcctc
     1981 ggcgagccgc gccgccacgt tcgcatacaa cccaaaccgg tccagccgcc ccgcgcgcac
     2041 gcggctcagg cgacctgcgt cgatcgccac gagaacgtcg aggtgctgat cctcgctgaa
     2101 ctcccgtgtc acgagcttgt gcgcccgtgc cgttgctttc caatcgatac gcgcaggcgg
     2161 gtctccccga acgtatccac gcagctggtg cagctcggag cctgcaccga tgacacgtcg
     2221 cgggcgcgcc cccgctggat taccccgcgg tcggcctcgt atcgtgttca gcgtgtcagg
     2281 tgcgaccgca agttgcgcgg acggatacag cacacgggac caccaagcga gcccaagcgg
     2341 acccagcagc cgtgccggga gcccgggcca gacttggcga ccgaggcgca cgggtaacag
     2401 gacgaacgga tcgcgcttga tctgcccggc ctcggcgacg attgttcgaa cgggcatctc
     2461 cgtccggatc ccagcagggg ctgccggggc gtactgcata cgcacgctgc gcagggtctc
     2521 attgcgaaac gcaaacgcgc ctgcctgctg tcgtccgaga aaagcgcgcg cggcggtctc
     2581 gacatcggcc ctcaccgagg cgcggcgaat ccggtacgcc tcgtatgcca agccgaggag
     2641 cagcagagcc gccggcagtc gccaggcttc ggagaggccc ggctcgctgg accagatgcc
     2701 ggcgatggcg agcaccgccg tgaggagaat cagaacgaag gcgcgctgcg caagatgcat
     2761 cggtcgcagt tcagcgcggc gcgggtgttt cggcgagcaa tctgtccacc acctgaaggt
     2821 ccgtcatacc ctcgagcgca gactccggtg tcagcaccag tctgtgggcg atgacccagg
     2881 cagccacttc cttcacatca tcgggagcta caaactcact gccgcgcagc gcggccgtcg
     2941 tgcgcgcggc cttgaccagt gcgatgacac cccgggtgga gaggcccaga gaaagccgcg
     3001 cttgcgtccg cgaggcctga gcaatatcca gcacgtaccc ggtgatcgct tcggaaacat
     3061 gcacgcgatc gacttccatt cgggcctgtt cgagtagggc agccggtagc gtgcccacct
     3121 cctggagtgc gtcgcgcgag gccgtgagtg tcttgccgta gcgcgccagg atttccgtct
     3181 ccgcgatccg ctccggatag ctgacgtcaa tacgcagcat gaagcggtcg agctgcgact
     3241 cgggcagcgg gtaggtccct tcgaactcgc gcggattctg cgtggccagc accaggaagt
     3301 cctccggcaa tgccagcacc tcgcgctcga cgctcacttg cctctcttcc atggcctcga
     3361 gcagagccga ttgtgtcttg ggccctgctc gattgacctc gtccaccagc acgacatccg
     3421 cgaacagagg gccggggcga aacacgaatg cccgccgctc gtcctcgaac acgtgaacgc
     3481 cgatgatgtc gctcggcatc agatctggcg tgccctgaat gcgcttgaac tcaccgccca
     3541 gggcgcgtgc cagagctttc gccagcagcg tcttgcccag acccggcacg ccctgcacga
     3601 ggacatgtcc gcgcgcgacg agtgcgatga ctagtgcgcg aatcgaagcg tcggcgccca
     3661 caatcacgcg tccgagttcc tgtcggagga agacttcgag ttcctgaaga gaagagctca
     3721 tgaatgaagt tgcctgttga ttttgcagat cagatcgtgc acccggacaa gcggtacacg
     3781 atcggaaacg taagcgtccg cgtaccagct cttgagctgt tgtatgtcgg cagcggcgat
     3841 ttgcggatga cgctccagcg cttcccaggg cgagcgctgc aggaatcggt caatcatgcg
     3901 tcgcgcggct gccgcggggg tcagcacgcg cgcgagaaat ccccccgtgg cccgtaccag
     3961 ctcctcttct cttggcgccg gtaaccgcag cgtaggcagc ctcaatctcg tggcccccag
     4021 cacccagcac agccacagcc cggcgagaat tccaatggtc agcctcagtc gcggatcgcg
     4081 atagaacttg gccggatcgt atgcagcacc caggccctga tgtgcatcgt cgaagagcac
     4141 cgcgccgtcg gaaccgatgt tcgccccgac gatgttggcc agaaggcgcg cgttatcctc
     4201 gagtccgatt gaccggttgg tgaaaagcga cccgagcgcg ctgacgatga tgcggccgct
     4261 gcccagagac cgcgtccaca gcacgccctc gcccgtagcc tgcgcatgcg ccagctcgag
     4321 aacaaaccca tcataaggca cctgcacgga ccaggcctgc gaggcgtaat cggagagtgc
     4381 caggccttct cgcaccccag tgaaataggc gtgcggacgg ttcgcaacga aagtctcgcg
     4441 gtcaggctcc gtcagcttgc gcgtggcggc gactatccga tcgctgagtg tgccgcgctc
     4501 ggcctgccgg ggagcccgcg atggctgccc ggagcgggca gcctcggatc gtctgtcgcg
     4561 cgctttgacc gtctcgaaat cgagccccgt cagcaggttc agatcccctg tggtcaggtt
     4621 gccgatcgaa aaagcccagt cagggctgtc cgaaagagcc gcgagcacca gcagagtgtt
     4681 gccggcccgg acccagcgat cgagtggccg gaactcctcg gtcttgtacg gcgcaatagc
     4741 gggcagtgtc acgatcagga tgttgcccgc tgcgggcaga tcgtgcctcg ccgcgagctc
     4801 atcgaagcga ttgcgcaacg atacggcgcg aatgtgcgca tccctgagcc actgcaggac
     4861 cgcaaagtag ccgtttccac ctcgctctgc ggtcgtcggc cgtgcaacct ccggctcgcc
     4921 agtcactgcc cggccatgca cgaacatggt cagaaaaagg agcagcgctc cgagcgcgca
     4981 cagcaaagtc gtgaatcgct ctctcatgca ggcgcctgcg cgctcagccg ctcgagcaat
     5041 tcgcgtccgc gcacgagcgg cacttccaga gcgtcggttg gaatcgcgtg gccggagaat
     5101 cgaacgcgct cggcggcgga ggcgagcgct gtgagcctgt gccgatcctc ggccgcgggc
     5161 actgccgcgg cccgcaccag ctccctcacc gtaagcgctc gggaaggggc gacgaagggc
     5221 ggccccgtaa gacgggtcac gatcagctcc agcaacactc ggggccgatc cccaacggat
     5281 gcgccctcaa ccacctccca gcccgtctgc gagctgatgg tgccctcgtg agcgctgcga
     5341 cgatgggcct ggcgacgacg tttctcgagc attcccgcca tgcgaaattc gttcagaacg
     5401 atcaggacgg caagcgtgac gaccgctgcg agggctgcgt aagcgagaag gtccagcatg
     5461 gactgcgaaa agccgattcg ggagatcaaa cggctgagcc agccctcgtt atcggtctgc
     5521 tcacgggagt cgaagatcga gcgcagccac cttttgacac gcatccacga ggtgacctgg
     5581 cccgcgcggg tctcgcccag ctgggccagg acacctgtca gcaattgcac gtggggtgtg
     5641 cgctcggtga ttctcgttgc aagctcgcgc gaagcgagat cgtgcaactc ccgcaggctc
     5701 gccgccgaca gatcgttgcg cggctcccgc cagccgcgcg gcagccacct gctccactca
     5761 ctctgctcca gctggcgggt gaggtccgga catcgcgccg cgatccgctc gtagccgatg
     5821 tccgtctggg catccagccg gctgatgcac tcatcgagac tcgccagcgc cgaactcggc
     5881 ggcgcgctct gcggcgtcgc cgcgggtgcc ggggccacct gggcattcac cggtgcgctg
     5941 gcccaggcag cgcacgcgag agagaggaac aatgccgcag tgcgtcccaa ggtcacgatc
     6001 gacggctcaa ttcaccggcg cactggcaat gcgccgctgc agatccgtgc cttcccggcg
     6061 cgcctccaga tcaccgtaca tcgccagcgc gatcgcactg tagaaggacg tcccgacggc
     6121 tccgagcacc gccgccatca cagccgacac cgcggcgaag acggccacat cggcgatacc
     6181 gaggagcggg gtcagcagtg cagccagcac gccgagcagg acatagaaca cggccagcat
     6241 ggcgatggcg accgagaaga tggtggacac gcgccaccag ttaccgcgga cgaggtgtgc
     6301 gctgtagcgc aggctggcga acactccctt gtcgcccatc agcaccgcga tgatgcagca
     6361 ggagagcacg accccgacat acgtcgcggg aacacacagc agcacgaacc cccacttgac
     6421 gtacgcgggc cgcaccacgg cgagtggaat gaaacaggcc ccgactgcca ggaagagcag
     6481 aattccggcc atgaaggtca caggcgcctt gcgcactcca gcggccagcg cacccttggc
     6541 cgaaagcgag cctctcaggg cggccccctg ccgcagcacg atgacgttga cgaaagcgat
     6601 cgagaaaaag actccgactc catagaggac gaaccaccac ggatcgccgc cgccaaactg
     6661 acgcagcggg cggtgtcgag cgatcaggta gatgtttggc agctggccgg cgacgatggc
     6721 gagcacgcca aagggaaggc aacccagaag agtgacccgg taaatccgga aagcagcatc
     6781 cagcacctcg ccgatgctct gggggcggga aggaggatac aaaaccgacg ccatccgcga
     6841 atattacgga ctatggatgg caatctcacc gtccgggcgc ttctggctca ggccatcgag
     6901 gatctgaggg gagcttccgg cgcgcccgct tcggtgccgc tcgatgccga gctcctcctg
     6961 ggtcatgccc tcgggatgag ccgcgcgcag atccgttcgc atccagaaac ggttccgacc
     7021 gacgaacgcg cgcagcgata ccggcgcctg gtgcagcggc gcaagctcgg agagccggtc
     7081 gcctacatca ccggccagcg tgaattctgg tccctgccgc tagcggtcag tccggcggta
     7141 ctggtacccc ggccggagac cgagttgctg gtcgagcgtg ctctcagcct gcatccgcag
     7201 cccaccgccc gggtgctcga gctcgggacc ggttccggtg ccatagctct cgcccttgcc
     7261 agcgagcgac ccgcatggct catcaccgcc accgacgttt cggaagaagc gctcgtcgcg
     7321 gcccgcacga atgctgccac ccatggcctg caacgcgtcg agtttctgcg tggcagctgg
     7381 ttcgcaccac tcgccgggcg cgtgttcgag ctggtggtga gtaacccgcc ctacgttgcc
     7441 gaagccgatt cggccctcca aagtccagcg ctcacttacg agccccggct cgcgctcgcg
     7501 ggaggcgtgg acggtctgct gtgcctgcgc gccatcatcc gggaagcacc gcagtacctc
     7561 gagcgtcgcg gttggctcct gctcgagcac ggcgcggagc aggccgacgc cgtagcacgc
     7621 gagcttgtcg ttcgcggctt cggtcacgta cgctcacatc gcgatctcgc cggccatgag
     7681 cggatgaccg aggctcagtg gctctgaccc aagccgcacg ctgcacacga ggacttatga
     7741 tcagattcga aacctctcac ggcaacttca cggtggagct cttccagaag gaagcccccc
     7801 tgagcaccgc aaacttccag aagtacgtcg atgacggcca cttcgacggc accattttcc
     7861 accgggtcat tcccggcttc atgatccagg ggggcgggct caccggggat ttcgccagca
     7921 agagcacccg cgagccgatc aagaacgagg cgaagaatgg gctgaagaac aagcgcggta
     7981 cgctgtccat ggcgcgcacc agcgacatca acagcgccac cgcccagttc ttcatcaacc
     8041 tggtggacaa cggctttctc gacaacagcg cgcgcgatta cggctacgcc gtcttcggtc
     8101 gggtcaccga aggcatggac acggtcgaca aaatcgcggc agtcaagacc ggcaggcgca
     8161 aggggtacca ggacgctccg ctcgaggacg tcgtgatcgt ctccgcgcgc accgttgaag
     8221 ggacctaagc aaagccgggg cagcgtcacc ggctggctcc tcaagtgggc catcttcctg
     8281 ctgatcgcct ttctcctgtg cagcacgctg ctggtgctga tgtttcgctg gctcaatccg
     8341 cccaccagcg cgttcatggt ggaggcgcga gtgcaggcga tgcgcgaggg cgatcgtcac
     8401 tatcgaaccg attacgagtg ggcaagtctg gagcacatct cgcccaatgc cgccctggcc
     8461 gtggtcgcct cggaggacca gcaattcccc tttcacgcag gcttcgatct gcactcgatc
     8521 cgcgaggccg ttcgcgccag tgatagaggc cacaggctgc gcggcgcgag cacgatctcc
     8581 cagcaggtgg ccaagaacct gttcctatgg aatggccata gcttcgtgcg caagggattc
     8641 gaggcctgcc tgaccgtgct catcgaggcg atctggccga aagagcgcat cctggagatc
     8701 tacctcaaca tcgcggaatt cggccgcggc atttatggtg tcgaggccgc ggcgcagcgc
     8761 ttttatcaca agcccgccgc aacactcacc gccagcgagg cggcgagcct cgcagcggtg
     8821 ctcccgaacc cgctgcgcat gcacccggac cggccctcgg cttacgtgct gagccgccgg
     8881 gagtggatca tgagtcagat gcgggacctc ggaggcccgg cgtacctgca ggccgtggag
     8941 aaggagctgc agccggcagc gcatcatccc tcgatgcgcg ccggcgaacc gctgcgggca
     9001 cgttgatcgt gagcgatagc tctcagcgcg gctcgtaacc cagattgggc gcgagccacc
     9061 tctcgagctc ctgcacggtc atctccttgc gccgtgcgta gtcctcaacc tgatcgcgat
     9121 cgatcttgcc gatatggaag taatgcgcct cgggatgacc gatataccac ccgctcacgg
     9181 cggccgtcgg atacatcgca aacgactctg tcaggcgaat gccaacattg cgctccggat
     9241 cgagcagctt ccatagcttc gccttctcgg tgtgatccgg acaggccggg tatcccggag
     9301 ccggacggat gcccagatac tcctcgcgga tcagttgtgc attggtcagt gactcgcgcg
     9361 cggcatatcc ccagtgctgc cgccgcaccc gctcgtgaaa gtgttccgcg caagcctcgg
     9421 ccagccgatc ggccagcgcc ttgagcatga tgctcgagta atcatcgtgg cctcgctcga
     9481 agcgtgcgag atgctcctcg attcctaccc ccgccgtaac ggcgaacgcg ccgatgtgat
     9541 cagccacgcc gctcgacctc ggggcaacat agtcagccag gcactcgtgc ggctgaccgg
     9601 gcgctttgcc cttctgctgg cgcaggaagc tcagcctggt caggacctcg ctccggcttt
     9661 catccacata gacctcgacg tcgtcgccga ccgagttggc ggggaacagc ccgatgacag
     9721 cccgggccgt cagccatgac tcggcgatca gctccttcag catccggcgc gcatcgtcat
     9781 aaagattgct ggccgcttcg cccaccttgg ggtccgtcag cacatccgga aacttaccgg
     9841 caaactccca ggcgttgaag aacggcatcc agtccacgta gccgagcaga tctttcagcg
     9901 gatagctgtc aaacacctgg atgcccggga cgcggggcgc ggccgcggcg taggccgtcc
     9961 agtcgatcct gcggcggttg gcgcgggcct gcgcgaggct cacttccggc gccttcgtct
    10021 tctttcccgc gtgctgttcg cgtcggcgct cgtgctcggc cgcgattttt tccagaaaat
    10081 ccgcgcgtga ggtctcggtc gagagcgtct ggcacacgcc tacggacctc gaggcatcct
    10141 tcacgtaaat gaccggcgat ttgtactgcg gcgcaatttt gaccgacgtg tgcgccggcg
    10201 aggtcgtcgc gcctccgatc agcagcggaa tctcgaagcc ctggcgctgc atctccttgg
    10261 ccacgttcac catctcatcc agcgagggcg tgatcaggcc cgagaggccg atgtaatcgg
    10321 cgttctcgcg ccgggcggca tccaggatct tctcgcacgg caccatcact cccaggtcga
    10381 tcacctcgaa attgttgcac tggaggacga ctccgacgat gttctttcct atgtcgtgca
    10441 cgtcaccctt gacggtcgcc acgacgattc gcccgttgga gcgcattgcg cccgtctggc
    10501 tcttttcgat gaacggaatc aggtgcgcga ccgcgcgctt catgacgcgt gcgctcttga
    10561 cgacctgcgg caggaacatc tttccggagc cgaacagatc gccgacgacg ttcatgccgt
    10621 ccatcagcgg cccttcgatc acctgcagcg ggcgttcgaa agtgagccgg gcttcttccg
    10681 tgtcctcaat gatgaagtcg tctatgccct tgaccagcgc gtgctcgagg cgcttggcga
    10741 ccggcagcga gcgccactgc gtatccgcct ggcgtttggc cccaccctcg cccttgaagc
    10801 gtgccgcgat ctccagcagc cgctccgtgc cgtccgggcg cctgttgaga accacatcct
    10861 cgaccgcctc gcgcagatcc ggggcgatct cctcgtagat ggcaagctgt ccggcattga
    10921 cgattcccat gtccatgccg gcggcaatcg cgtgatagag aaataccgaa tgcatcgcct
    10981 cgcgcacggg atcgttgccg cggaatgaga acgagacgtt gctcacgccg ccgctgacga
    11041 gcacatcggg aagcgcggcc ttgatctgcc gcgtggcctc gatgaaggcg agcccgtatc
    11101 cgttatgctc ctcgatgccc gtggcgatcg cgaagatgtt ggggtcgaaa atgatatctt
    11161 ccggcagtac gcccgcacgc gtcgtgagaa tgtcgtagca gcgcttgcag atcgccacct
    11221 tgcgctcgac cgtatccgcc tgaccctgct cgtcgaaagc catcacgacc atcgccgcgc
    11281 catagcgtcg caccttgcgg gcatgctcga tgaaggcctc ttcgccttcc ttcatgctga
    11341 tcgagttgac gaccgccttg ccctgcacgc atttgagccc ggcc
//
LOCUS       DASRHN010000151         6589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_542607, whole genome shotgun sequence.
ACCESSION   DASRHN010000151 DASRHN010000000
VERSION     DASRHN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542607"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFB37_05845"
     CDS             <1..500
                     /locus_tag="VFB37_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZO22010.1"
                     /translation="HRGHFLSDDLTEQVGTFALIPQLVRKVAVPVIATGALADRAGIA
                     AALRLGAAAVQVGTAFLLCPEARTGAAHRAALKSDSSAHTAITNVFSGRPARGIVNRL
                     IRERGPMSALAPAFPLANAAVAPLRAAAEARGSGDFSPMWAGQNATGCKEIAAAALVR
                     ELTSA"
     gene            complement(516..1859)
                     /gene="aceA"
                     /locus_tag="VFB37_05850"
     CDS             complement(516..1859)
                     /gene="aceA"
                     /locus_tag="VFB37_05850"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZO22011.1"
                     /translation="MTLQRSLLPTADELRNTWRDSPRWRGVERTYRAEDVIRLRGTVA
                     IEHSIARLTAEKLWRYMNEKPFVNALGALTGNQAMQQVKAGLDAIYLSGWQVAGDANL
                     AGEMYPDQSLYPADSVPAVVRRINSTLKRADELHHAEGDDSIDWFKPIVADAEAGFGG
                     VLNAFELMKQMIEAGAAGVHFEDQLSSAKKCGHMGGKVLVPTQEAINKLAAARLAADV
                     CNTPTVLIARTDAESANLLTSDVDERDRSFIDTSKGRTAEGFFCVKAGLDQAIARGVA
                     YAPFVDALWCETGKPDLEEARRFAEGIHKHYPGKLLAYNCSPSFNWKRKLDDATIAKF
                     QRELGAMGYKFQFITLAGFHALNFSMFELARGYKSHQMTAYVTLQQAEFAAESSGYTA
                     TKHQREVGAGYFDDVTQTITAGTSSITALKDSTESEQFHGHETAAAGTGTKKGVR"
     gene            complement(1894..3525)
                     /gene="aceB"
                     /locus_tag="VFB37_05855"
     CDS             complement(1894..3525)
                     /gene="aceB"
                     /locus_tag="VFB37_05855"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HZO22012.1"
                     /translation="MNAQPQHMTYSREQAGPRVEIAGHWVERSEEVLTPLALQLLASL
                     HRRFNPKRLELLEARERRQAELDGGAVPEFLPGTAALAAGEEVEEWRVGAIPADLLDR
                     RVEITGPVDRKMIINALNAPVQAFMADFEDSCAPTWDNIVRGQVNLMDAVRGTISYAD
                     PQTGKVYRLAERTATLIVRPRGWHLPEKHVRIGGEVVSGALFDFALFIANNHEALARR
                     GTGPYFYLPKMESHLEARLWNDVFLAAQDALGIPRGTIKATVLIETILAAFEMDEILF
                     ELREHCVGLNCGRWDYIFSFIKKFRNWPDLLLPDRASVTMDRHFLKSYVDLLVKICHR
                     RGAHAMGGMAAQIPIRNDVLANEAAMERVRADKLREVRAGHDGTWIAHPGLAAIAREA
                     FDSVGPNQLQVMRSEVRVTPADLLKVPDGNITEQGLRACIRVGVQYIESWLRGNGCVP
                     LYNLMEDAATAEICRAQLWQWIRHGARTSDGRTVSVERFDALLTDELDRIHDEVGAAR
                     LAGGVFPTASRLFAEMIKQDTFDEFLTLPAYDLLA"
     gene            3637..5190
                     /locus_tag="VFB37_05860"
     CDS             3637..5190
                     /locus_tag="VFB37_05860"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HZO22013.1"
                     /translation="MSTLLRKGHFLGPKLRAIRKRNGLTLEELSARCIQRDVTLAPSV
                     SYLSMIESGKRAPSQELLGLLAAVFQRSPSWFLDENADVESTGVAAPAGGAAQVPLEP
                     AFLFSKNVLQAAIPELLVQTGTTGRQFAHLLIRSHQEISRNDFPDLERAADSIGERRF
                     PLSVDDLMRLCKRHGLEIRWFERKPVLVRDKDRDVRSMVRSFFEAPKVVYANRALQSD
                     PARLKFDLAAHLAHKVLHGGDGLKSAHATGGEMGGSPESGSSAAGMNAQDVLHAWRDF
                     ECSFFAGALLAPRAPFRRFLMRERYRVEAGVKLELTPAVIMRRMTKVSPYPYWHFFDA
                     YPPGYLRAVYRGNGIPLPWGNLAQVSDPCPNWAVFRMIDDGHRGESGSQISVLRDGER
                     SLLYCCHSLRARDMAGNPHVLSVGVDLAPALNSNGTGTEELIDSIFRECLHRRGEARI
                     PRAAVQMLEAVANVLNIAWVADALTQPARIICPRSTRCPRPERCEGAPPSRAREIADV
                     REEILGRPE"
     gene            complement(5240..>6589)
                     /locus_tag="VFB37_05865"
     CDS             complement(5240..>6589)
                     /locus_tag="VFB37_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO22014.1"
                     /translation="LPLTGWLTRRFGQVKLFVWSVGLFTLASFLCALAPTLGMLILFR
                     VLQGAVAGPMMPLSQALLLSSYPKAKAGAALALWGITTLVAPVVGPVLGGWITDNIAW
                     PWIFYINVPVGIVAGLVTWFIYQKRESPTARIPIDKIGLALLVLWVGCLQVLLDKGKD
                     LDWFNSGQIITLAVISVIGFAFFLVWELTEKHPVVDLSLLTRRNFLVSTIAMLLAYGL
                     YFGNVVLLPLWLQQYMGYTATLAGLVLAPVGLLAIFLTPVIGRYVNRIDPRVLVTGAF
                     GVFALVLFMRDHFNTDADIGTLLVPTLIQGVAMACFFIPLISISLSGLEPERIPSASG
                     LFNFARILAGSFGTSITTTLWDRRATLHHAQLVEHLTTGSTASTQALANLQSGGLGSQ
                     QSYDLLNRLVDQQAFMLSANDIFYASGVLFLALVVVIWFARPVLGSAGAAAAASGAH"
BASE COUNT         1167 a         2127 c         2135 g         1160 t
ORIGIN      
        1 gccatcgggg gcattttctg agcgacgatc tgaccgagca ggttgggacg ttcgcattga
       61 ttccgcagct cgtgcgaaag gtggcagtgc cggtgatcgc caccggcgcg ctcgccgatc
      121 gggccggcat cgccgcggcg cttcgccttg gagccgctgc cgtacaggtc ggcaccgcgt
      181 tcctgctttg ccccgaagcc cgtaccggcg ctgcgcaccg ggctgcgctc aagagcgatt
      241 cgagcgctca tacagcgatc accaacgtat tcagcggccg gccggcgcgc ggaatcgtca
      301 atcggctgat tcgagaaaga ggtccgatga gcgcactcgc accggccttt ccgctggcca
      361 atgctgcggt ggcgcccttg cgcgcagcgg ccgaggctcg cggcagcggg gacttctctc
      421 cgatgtgggc gggccagaac gccacgggct gcaaggagat tgcagccgcc gcgctcgtgc
      481 gcgaactcac ctcggcgtga gttgacccgc gttcgctatc gcacgccttt cttggtcccc
      541 gtgccggcag cggccgtctc gtgaccgtga aattgctcgc tctcggtgct gtccttcagc
      601 gcagtgatcg aggaggtgcc ggccgtgatc gtctgggtga catcgtcaaa atacccggct
      661 ccgacttccc gctggtgctt ggtcgcggta tagccgctgc tttcggcggc gaactcggcc
      721 tgctgcagag tcacgtatgc ggtcatctgg tgtgacttgt agccgcgcgc cagctcgaac
      781 atggagaagt tcagcgcgtg gaagcccgcc agggtgatga actggaactt gtagcccatc
      841 gcccccagct cgcgctggaa tttggcgatg gttgcatcgt cgagcttgcg cttccagttg
      901 aaggacggcg agcagttgta ggccaggagc ttgcccgggt agtgcttgtg gatgccttcg
      961 gcgaagcggc gggcctcctc cagatccggc ttgccggtct cgcaccacag cgcatcgacg
     1021 aagggtgcgt aggcgacccc acgcgcgatc gcctgatcca ggccggcctt gacgcagaag
     1081 aagccctccg cggtccgccc tttcgaggtg tcaatgaacg agcgatcccg ctcatcgacg
     1141 tccgaggtca gtaggttcgc ggactccgca tcggtgcggg cgatgaggac ggtcggcgta
     1201 ttgcagacgt ccgctgcgag cctggcggca gcgagcttgt taatggcttc ctgggtcggt
     1261 acgaggactt tgccccccat gtggccgcat tttttcgccg acgagagctg gtcttcgaaa
     1321 tgcacgccgg cagcaccggc ttcgatcatc tgtttcatca gctcgaaggc gttgagcacg
     1381 ccgccgaagc cggcctcggc atcggccacg atgggcttga accagtcaat cgagtcgtcc
     1441 ccttccgcat gatgcagctc atccgcgcgc ttgagcgtcg agttgatccg ccgcaccacg
     1501 gccgggacgg agtcggccgg atagagcgac tggtccggat acatctcgcc ggcgagattc
     1561 gcatcacctg cgacctgcca gcccgagagg tagatggcat cgagccccgc cttgacctgc
     1621 tgcatggcct ggttgccggt cagggctcca agcgcattca cgaaaggctt ctcattcatg
     1681 tagcgccaga gcttctcggc cgtcagccgc gctatcgaat gctcgatggc caccgtgccg
     1741 cgcagccgga tgacatcttc tgctctgtag gtgcgctcca caccacgcca gcgggggctg
     1801 tcgcgccacg tgttgcgcaa ctcatcggcc gtgggaagaa gcgatcgctg caacgtcatc
     1861 tgtgagtctc cctcgaaata atgtggtcgg ctgtcaagcc agcagatcgt atgccggaag
     1921 cgtcaggaac tcatcgaacg tgtcctgctt gatcatctcc gcgaacaggc gcgaggcggt
     1981 cgggaacact ccgccggcga ggcgcgccgc gccgacctcg tcgtgaatcc tgtcgagctc
     2041 gtcggtcagc agcgcatcga agcgctcaac gctcacggtg cgcccatcgc tggtgcgcgc
     2101 gccgtggcgg atccactgcc agagctgcgc gcggcagatc tcggcagttg ccgcatcttc
     2161 catgagattg taaagcggaa cgcatccgtt gcctctcagc caggactcga tgtactgcac
     2221 gccaacccgg atgcaggccc gcaggccctg ctccgtgatg ttgccgtccg ggaccttgag
     2281 cagatccgcc ggggtgaccc ggacttccga gcgcatcacc tggagctggt tgggccctac
     2341 ggagtcgaat gcctcgcggg caatggctgc gaggcctggg tgcgcgatcc aggtgccatc
     2401 gtgacctgcg cggacctcac gcaacttatc ggccctgact cgttccatgg cggcttcgtt
     2461 cgccagcacg tcattgcgaa tcggaatctg ggcggccatg ccgcccatgg cgtgagctcc
     2521 gcggcgatgg cagatcttga ccagcagatc gacatacgac ttcaggaagt gacggtccat
     2581 ggtcaccgag gcgcgatcgg gcagcagcag atcgggccag ttccggaact tcttgatgaa
     2641 gctgaagatg taatcccagc gtccgcagtt caggcccacg caatgctcac gcagctcgaa
     2701 caggatctca tccatctcga aggcggcgag gatcgtttcg atgagcactg ttgctttgat
     2761 ggttccccgc gggatgccga gcgcgtcttg agctgcgagg aagacatcgt tccacagccg
     2821 tgcctccaga tggctttcca tcttgggcag atagaagtac ggaccggtac cgcggcgggc
     2881 caaggcttca tgattattgg cgatgaagag cgcgaaatcg aacagcgctc cggagaccac
     2941 ttccccgcct atgcgcacat gcttctcggg cagatgccag ccgcgcgggc gcacgatcag
     3001 ggtcgcagtg cgctcggcaa gccgatacac cttgccggtc tgcgggtccg cgtagctgat
     3061 ggtgccgcgt acggcatcca tcaggttgac ctgcccgcgc acgatgttgt cccacgtagg
     3121 cgcgcaagag tcctcgaagt ccgccatgaa cgcctgcacg ggcgcattca gagcgttgat
     3181 gatcatcttt cggtccaccg gaccggtgat ctcgacgcgc cgatcgagca ggtcagctgg
     3241 aatggcccca acacgccact cctcaacctc ctcgccagcg gccagcgccg cggtgccggg
     3301 caggaattcc ggtacggcgc cgccgtcgag ctccgcttgg cggcgctcac gagcctcgag
     3361 cagctccagg cgcttcgggt tgaaacggcg atgcaggctc gccaggagtt gcagcgccag
     3421 gggcgtcagc acctcctcgg accgctcgac ccagtgaccg gcgatctcga cgcgggggcc
     3481 ggcctgctca cgtgaataag tcatgtgctg cggttgcgcg ttcatcctgc gagcataact
     3541 cgctcgtagc ggaatttgac gacggaaact tcacactgtt aatttcacaa ggccggcgga
     3601 cgactgcaac gctaccgcct gcgtcaggag gtcatgatgt ctacgctgct tcgcaaggga
     3661 cacttcctgg gtcccaagct gcgcgccatc cgcaagcgta acggtttgac gctcgaagag
     3721 ttgtcggcac gctgcatcca gcgcgatgtg accctggcgc cttcggtttc atacctgagc
     3781 atgatcgaga gtggcaagcg cgccccctcg caggagctgc tcggtctcct ggccgcagtc
     3841 ttccagcgca gtcccagttg gtttctcgat gagaacgcgg atgtcgaaag cactggcgtc
     3901 gcagcgcccg ccggtggcgc ggcacaggtt ccgctggagc ccgctttcct gttctcgaaa
     3961 aacgtccttc aggcagcgat cccggagctg ctggtccaga cgggcactac cggtcgccag
     4021 ttcgcccatc ttctgatccg ctcgcaccag gagatctcgc ggaacgactt tccggatctg
     4081 gagcgcgccg cagacagcat cggcgagcgc cgcttcccac tctcggtgga cgatctgatg
     4141 cggctgtgca agcgccatgg gctggagatc cgttggttcg agcgcaaacc ggtgctcgtg
     4201 cgcgacaagg atcgagacgt gcgcagcatg gtgcgctcgt tcttcgaggc gccgaaggtc
     4261 gtgtatgcga accgggctct gcagtcggac cctgcgcggc tgaagttcga cctcgcggcc
     4321 catctggcgc acaaagtcct gcatggcggt gacggtctga agtccgccca cgccaccggg
     4381 ggggagatgg gcggcagccc ggagagcggc tcgagcgcgg ccggcatgaa tgcgcaggac
     4441 gtgctgcatg cctggcgcga cttcgagtgc agcttcttcg cgggtgccct cctcgctccg
     4501 cgcgcaccct tccgccgctt cctgatgcgc gagcgatatc gggtcgaggc tggggtcaaa
     4561 ctcgagctga cacccgcggt catcatgcgg cgcatgacca aggtctcgcc gtacccctac
     4621 tggcacttct tcgatgcgta tccccccggc tatctgcggg ccgtgtaccg cggcaacggc
     4681 attccgctgc cgtggggaaa tcttgcgcag gtgagtgacc cgtgtccaaa ctgggcggtg
     4741 ttccggatga tcgacgacgg ccaccgcggc gaatccggct cgcagatctc cgtgctgcgg
     4801 gatggggagc gctcgctgct gtactgctgc cactcgctgc gggcccgcga catggccggc
     4861 aatccgcacg tgctatcggt cggcgtggat ctcgcgcccg ctctgaactc gaacgggacg
     4921 ggcaccgagg agctgatcga ctcgatcttc cgggaatgcc tgcaccggcg cggcgaagcc
     4981 cgtatccctc gagccgccgt gcagatgctg gaagcggtgg ccaacgtgct caacatcgcc
     5041 tgggtcgcgg atgcgctcac tcagccggcg cgtatcatct gcccacgcag cacccgctgc
     5101 ccgcgtcccg agcgctgcga aggcgccccg ccttcgcgcg cacgcgaaat tgccgacgtg
     5161 cgggaagaga tcctcggaag accggaatga ctcccgggtg aattccgcgg cggccgcgct
     5221 ttttctggca actctcggat cagtgtgcgc cgctcgcggc cgcggctgcc ccggccgacc
     5281 cgagcacggg ccgcgcaaac cagatcacga ccacgagtgc gagaaacagc acccctgagg
     5341 cgtagaagat atcgttggcg gacagcatga aggcctgctg atccacgagc cggttcagca
     5401 ggtcatagct ctgctgcgaa cccaaaccgc cggactgaag gttggccagc gcctgcgtcg
     5461 atgccgtgct accggtggtc aggtgctcga caagctgcgc gtggtgcagg gttgcacggc
     5521 ggtcccacag cgtggtcgtg atcgaggtcc cgaaggagcc ggcgagaatg cgggcgaaat
     5581 tgaacagccc cgaggccgag gggatccgct cgggctccag tcccgagagg ctgatggaaa
     5641 tcagcgggat gaagaagcac gccatggcca cgccctgaat cagggtcggc acgagcagcg
     5701 tgccgatgtc cgcatcggtg ttgaaatggt cgcgcatgaa cagcacgagg gcgaagacac
     5761 cgaatgcccc ggtcaccagc actctggggt cgatccggtt cacgtagcgg ccgatcacgg
     5821 gcgtcaggaa gatcgcgagc agcccaacag gggcgagcac caggccagcc agggtggcgg
     5881 tgtaacccat gtattgctgc aaccacagcg gcagcagcac cacgttgccg aagtacagtc
     5941 cataagcgag caacatcgcg atcgtgctca cgaggaagtt acgtcgcgtg agcagactca
     6001 agtccaccac cggatgcttc tcggtcagct cccataccag gaagaacgcg aacccgatga
     6061 ccgagatcac cgccagcgtg atgatctgtc cggagttgaa ccagtcgaga tccttcccct
     6121 tgtcgagcag gacctgcagg catccgaccc acaacaccag cagagccagg ccgatcttgt
     6181 cgatcggaat gcgggcggtc gggctttcgc gcttctggta gatgaaccag gtgacgaggc
     6241 ccgcgacgat gcccaccggc acattgatgt agaagatcca gggccacgcg atgttgtcgg
     6301 tgatccagcc cccgagcacc ggccccacca ccggtgcgac cagggtcgtg atgccccaga
     6361 gagcgagcgc agcacctgct ttcgccttcg ggtagctcga gagcagcagc gcctgggata
     6421 gcggcatcat ggggcctgcg acggcaccct gcagcacgcg gaagaggatc agcatgccca
     6481 gtgtgggcgc gagcgcgcac aggaatgagg cgagcgtgaa cagcccgacc gaccagacga
     6541 agagcttgac ctggccgaac ctgcgcgtga gccagcccgt gagcggcag
//
LOCUS       DASRHN010000152         4849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1699441, whole genome shotgun sequence.
ACCESSION   DASRHN010000152 DASRHN010000000
VERSION     DASRHN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699441"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFB37_05870"
     CDS             <1..576
                     /locus_tag="VFB37_05870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO22015.1"
                     /translation="RHGLKRGRYFLQVSRIVPEKRQLDLIRAFAQARPPGWQLALVGA
                     GTGEYAEKVEAAARAEGVVMTGFQKGAALQQLYSHAGAFVLPSSHEGLPIAILEALSH
                     GLPVIASDIPANLEIGLDSSSYFPVGDTAALAELMTKLTQAPIDVGAIEARRQWVART
                     YDWDRISAQTHAVYERVLGHPSRMHEHRAST"
     gene            complement(666..1949)
                     /locus_tag="VFB37_05875"
     CDS             complement(666..1949)
                     /locus_tag="VFB37_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22016.1"
                     /translation="MMRSKLIVSALAGLLGVAAMMGADAGQPNPPARVCVNNRCVTPP
                     PTGGRIKWNPGHYMASDGILYPGRTVSSFSGELKDLNWDNIVGYRVFTTWGALEKAQD
                     QYDFSALDGILQYLKTHYDQPKHMVVAILPASFGNSLGNDDNRIIPMYIQNSSAYGPS
                     PTPGSYGWWGPSSHGKSNSAYSAALWRPAVMSRFIKLMQALGAHYNNEPYFEGIMFQE
                     DSGPPGAAAVSDPKPNDYSVSGMVTQYQNLLTAMTAAFPNTNVIFENTWGVNSTTATQ
                     DLEVWMVQHRIAPGSADVLGQTAFDYKDNHIYLNFGMAAYLGVGTTGTPYSGGDLRDQ
                     SRAMLDIEASDMAGSYFSRWGGPYSTADLLNALNQTYKASHAFWTHLVGSEAVPGGGT
                     VSSSNPDAVWSKVAPYLNSHPLTNTSYPPNYPSSN"
     gene            2203..3504
                     /locus_tag="VFB37_05880"
     CDS             2203..3504
                     /locus_tag="VFB37_05880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO22017.1"
                     /translation="MSFKLLQRAADALAGHPARSAALIFVLALLPRILMALHLPQAVL
                     WPDGARYMLVADHLLAGQGFGSLQDNALSVPTQPVLLAAVRFLSNNSFLALRIFFAVV
                     GAATCAVGYELARRLFGPMSALLAGVALALYPPLIYLSALFEYPQPFYMLLMALVFLT
                     YLEFTRSRRISSLLLCGLCLGLSILTVPTVLLFVPLLLGCLLLEQRRRFWAYAPLIVL
                     AIALPVATWSVRNEAAYGTPILVNKAGGFNFWMANSETYYELGKIGVTPPCARGYEQT
                     SFCVQFRALHRQLKSQHPTANQLILQEDAGCWQQGIRFLRSDLGRSVSLTARKFLLYW
                     SPLPDAVHSGGSYEGRSASWIAALSYLPVLVLGIIGIVLSGRYWRELLPIYGYFAVFM
                     SVYSIFMPTTRYRLPLDFFLIIFSAYTLSRLLEAHPKASPP"
     gene            3501..4394
                     /locus_tag="VFB37_05885"
     CDS             3501..4394
                     /locus_tag="VFB37_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO22018.1"
                     /translation="MNLRDAGYFTAGLGFLALAKAKHVLRGYSSPKPFDLSQTDRCVA
                     YDTQVVRDWLRELARYSGGDTDLRGKDILELGPGSDLGVGLLLLAQGATSYNACDVHD
                     LAKHAPPAFYEALFSSLSGAEGVNIATLREALRESLAGRPSRLNYVVRSDFDIAAAFG
                     PATIDMVFSQAAFEHFDDIDAVVRRLTRVCRPGARIVAEIDLKTHSRWVRDHDPNNIY
                     RFPDPLYNMFRFRGIPNRVRPYQYAQAFERNGWIDIQMRPLTEVQGANGVSTGGFSRR
                     FRDEVNQMKHLTIALCARRPA"
BASE COUNT          811 a         1601 c         1505 g          932 t
ORIGIN      
        1 cggcacggac tcaagcgggg acgctatttc ctgcaggtca gccggatcgt ccccgagaag
       61 cgtcagctcg acctgatccg tgcgttcgcg caggcgaggc ctccgggctg gcagctcgcc
      121 ctcgtcgggg ccggcaccgg agagtacgcc gagaaggtcg aagccgcggc ccgcgccgag
      181 ggcgtggtca tgaccggctt ccagaaagga gcagcgctgc agcagctgta ttcgcacgcg
      241 ggggccttcg tgttgccctc ctcacacgag ggtctgccga tcgccattct cgaggcgttg
      301 agccacggct tgccggtgat cgccagcgac attcccgcca atctcgagat cgggctcgac
      361 agctcgagtt acttcccggt cggcgacacg gcggcgctcg ccgagctcat gacgaagctg
      421 acgcaggccc cgatcgatgt gggggccatc gaggcgcggc ggcagtgggt cgcacgcact
      481 tacgactggg accgcatctc agcccagacc cacgccgtgt atgagcgcgt gctcggccac
      541 ccctcgcgga tgcatgagca ccgcgcttcc acctgacctg tccgcgaaaa cgcacgcatc
      601 gaggtgcccg gcagagcccg cacgctgatg tgctcggccc gggtcaaccc tcacggagcg
      661 ggcgatcagt tactcgaagg ataattgggc ggatagctcg tgttggtcag cggatggctg
      721 ttgaggtacg gcgccacttt cgaccagacc gcatccggat tcgagctgct gaccgtgcca
      781 ccgcccggca ccgcctcgct gccgaccaga tgcgtccaga aggcgtgcga ggccttgtac
      841 gtttgattga gcgcgttgag cagatcggcg gtggagtacg ggccgcccca gcggctgaaa
      901 tagcttccgg ccatgtccga ggcttcgatg tcgagcatcg cgcgcgactg atcgcgcaga
      961 tcgccgccac tatagggcgt gccggtcgtg ccgacgccca ggtaagcggc catgccgaag
     1021 ttcagataga tgtggttgtc cttgtagtcg aatgccgttt gtcccagcac atcggcggag
     1081 cccggggcga tacgatgctg caccatccag acctcgagat cctgagtggc cgtggtgctg
     1141 ttgacgcccc aggtgttctc gaagatcacg ttggtgttgg gaaacgcagc cgtcatcgcg
     1201 gtcagcagat tctggtactg cgtcaccata cccgagacgg agtagtcatt cggcttggga
     1261 tcggaaacgg ccgcggcgcc cggagggccc gaatcctcct ggaacatgat gccctcgaag
     1321 tacggctcgt tgttgtaatg cgcacccaga gcctgcatca gcttgatgaa acggctcatc
     1381 acggccggac gccacagcgc cgcgctgtag gcgctgttgg acttgccatg ggagctcgga
     1441 ccccaccagc cgtagctgcc cggggtcggg ctcggtccgt acgcactgct gttctggatg
     1501 tacatcggga tgatgcggtt atcgtcattc ccgagagaat tgccgaagct cgccggcagg
     1561 atggccacca ccatgtgctt gggctggtcg tagtgcgtct tgaggtactg cagaatcccg
     1621 tccagggcgg agaagtcgta ctggtcctgg gccttctcga gcgcgcccca ggtggtgaac
     1681 acgcgatagc cgacgatgtt gtcccagttg aggtccttca gctcgcccga gaagctgctc
     1741 acggtgcggc ccggatagag gatgccgtcg gaggccatat agtggccggg attccacttg
     1801 atgcggcctc cggtgggcgg cggcgtcaca cagcggttgt tcacgcacac gcgcgccggc
     1861 ggattgggct ggccggcgtc tgcgcccatc atggcggcca cgcccagaag ccccgcgagc
     1921 gcggagacaa taagtttact tcgcatcatc ccaggacccc cgccgacata ccccggcgcc
     1981 aaacgagact tgtgtcacac accgcgttca aactcgcgca gtgtgcaaaa aaagcgcaag
     2041 tctcaacata tggcggcaaa gtatcgtcgg ccaacaggtc agtcaactgt cgaaccattg
     2101 cgacaccgcg gcccagggcc tcgcgcgcgt tgcgagttcc cgcaagttga gacgtacatc
     2161 gcaaatcgta tgcgctgcca atacatgtaa cccctgcgaa acatgagttt caagctcctg
     2221 caacgtgctg cggatgcgct cgccggccac cccgctcgca gcgcagcact gattttcgtg
     2281 ctcgcgctgc tgccacgcat cctgatggcg ctgcatctgc cgcaggcggt tctgtggccc
     2341 gacggcgcgc gctacatgct ggtggccgat catctgctcg ctggccaggg tttcggcagc
     2401 ctgcaggaca atgcgctctc ggtgccgacc cagcccgtgc tgctcgccgc cgtgcgcttc
     2461 ctatccaaca acagcttcct ggccctgcgg atcttcttcg ccgtggtggg cgcagcgacc
     2521 tgcgccgtgg gctacgagct cgcccggcgg ctgttcgggc ccatgagtgc gctgctcgcc
     2581 ggggtggcgc tcgcgctcta cccgccactc atctacctct cggcgctgtt cgagtatccg
     2641 cagcccttct acatgctgct catggcttta gtctttttga cctacctcga gttcacgcgc
     2701 agccggcgca tcagcagcct gctgctgtgc ggcctgtgcc tcgggttgag catcctcacc
     2761 gtgcccacgg tgctgctgtt cgtcccgctg ctcctcggct gtctgctgct cgagcagcgg
     2821 cggcgcttct gggcttacgc acccctgatc gtgctcgcga tcgcgctgcc ggttgccacc
     2881 tggagcgtac gcaacgaggc cgcctacggc acgccgatcc tggtgaacaa ggcgggcggc
     2941 ttcaatttct ggatggccaa cagcgagacg tactacgagc tcggcaagat cggtgtcacc
     3001 ccgccgtgcg ctcgcggcta cgagcagacg agcttctgcg tgcagttccg cgcgctgcat
     3061 cggcagctga agagtcagca tcccacggcc aatcagctca ttctccagga ggatgccggc
     3121 tgctggcagc agggcatccg cttcctgcgc tcggatctgg ggcgctccgt gagcctcacc
     3181 gcgcgaaaat ttctgctgta ctggagcccg ctgccggatg cggtgcacag cggtggttcc
     3241 tacgaggggc gctcggcgag ctggatcgcc gccctctcct atctgccggt gctcgtgctg
     3301 ggcatcatcg gcatcgtgct ctcaggccgc tactggcgcg agttgctgcc gatctatggc
     3361 tatttcgccg tgttcatgag cgtctactcg atcttcatgc ccaccacgcg ctaccggctg
     3421 ccactcgatt tctttctgat cattttctcc gcgtacactt tgagccgcct cctcgaggct
     3481 catccaaaag cgagtccccc gtgaatcttc gcgatgcagg ctatttcacc gccggtctcg
     3541 gcttcctcgc cctcgccaag gccaagcacg tcctgcgcgg ctactcctca cccaagccct
     3601 tcgacctgtc gcagaccgat cgatgcgtcg cctacgacac gcaggtggtg cgcgactggc
     3661 tgcgcgagct cgctcgctac agcggcgggg ataccgatct gcgcggcaag gacatcctcg
     3721 agctcggacc gggctcggat ctgggtgtgg ggctgctgct gcttgcgcaa ggggcgacct
     3781 catacaacgc ctgcgatgtg cacgaccttg ccaagcacgc accgcccgct ttctacgaag
     3841 cgctcttcag ctccctgtcg ggcgccgaag gggtgaacat cgcaacgttg cgcgaagcgc
     3901 tgcgtgagtc gctcgcagga cgcccgtcgc ggctcaatta tgtggtgcgg agcgatttcg
     3961 acatcgcggc cgcgttcggc ccggccacga tcgacatggt cttcagccag gccgcgttcg
     4021 agcacttcga cgatatcgac gccgtggtcc ggcggctgac gcgcgtctgc cggcccggcg
     4081 cgcgcatcgt cgccgagatc gacctgaaga cccactcgcg ctgggtgcgc gatcacgatc
     4141 ccaacaacat ctatcgtttc ccggacccgc tttacaatat gtttcgtttc cgtggcatcc
     4201 cgaaccgggt gcggccctat caatacgcgc aggccttcga gcgcaacggt tggatcgata
     4261 tccagatgcg gcccctcacc gaggtgcagg gcgcgaacgg cgtctccacc ggaggcttca
     4321 gcaggcgctt tcgcgacgag gtgaatcaga tgaagcacct gacgattgca ttgtgcgcgc
     4381 gccggcctgc ctgactactg gaagaatcgc ctgcggcgat tcttcggaca tcggctgcga
     4441 ttccgggcgc gaaagacgcg actttcgcgc cctgcgctgc gggcaatcag gcatgcgcga
     4501 agcgggaacc cttatgtcaa gcttcaatgt acacataagt cgttgaatcc gcggcggagc
     4561 gtgatcgaca tttcttccta tgcagaccat aattcccgcc tgccttcttc aggcgcggga
     4621 cttgcgcaca aaaattgctc tggcaacgca ggtcccttgc gttgagccag agcttcagcg
     4681 aaagggcatc gcggccgttt ccggggtcgg ccgccagcag cagcttccaa ccgccgcgtt
     4741 tccaggagct ttgaatggac ctgacgatca tctccacgta tcggtgcaac tcgcgctgcc
     4801 agatgtgcta catctggcag aacccgaccg atccgcgcga ggaggtctc
//
LOCUS       DASRHN010000153         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1207413, whole genome shotgun sequence.
ACCESSION   DASRHN010000153 DASRHN010000000
VERSION     DASRHN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207413"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFB37_05890"
     CDS             <1..1502
                     /locus_tag="VFB37_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZO22019.1"
                     /translation="QLHTLDRAGAGCPGVAVPSVQEGVPELMRGKGKGIASTLVAAAA
                     GISVAACGGGAGATQAASSPTVTSEADTILHMDQVRAAGYTGAGVRVGVISTGVVNLA
                     SYQTAGALPTSLYVTQNAAGMLDEGSWMLELVHQHAPDAVLGFCDGLDLDFDGCIKDL
                     ANNFHADVIVDDILFSGQFYPDATADLVTQLEAANDRLVFIHLAGNEQKGGYWQGPFI
                     PTEAVLNGATETVLDFGLASGAASDPFNSISVPAGAHLILIMNWNDPPHGTANHALSV
                     HLLGDVSQELAHASGQSDPTVRLDYVNTGASAQTVRLAVSLDEGTPGGLAIQVTEGSP
                     TCNIECQPMAHESAGLAGGTIGDFPDALVVGATSAMSPRQLEAWTNRGPFRIDFQSTS
                     DSAAPDGYDYARLATPLRISKPDLVAPDCVTVPFSSGTALSNDTFCGTSAAVPAIAGA
                     AALLESAGFNRSRVLRALRTTAVRLGASAWDPGYGFGLADAAAAWRSGG"
     gene            1550..3493
                     /locus_tag="VFB37_05895"
     CDS             1550..3493
                     /locus_tag="VFB37_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HZO22020.1"
                     /translation="MERFIRAPGKLLAACLLSLLLPLVASAGGECRVVRFAEFPVTMS
                     GTRPLIAGSINGVDALFMADSGAFFSVISHERAESYKLLMGTLPSGLVVSGIGGTAGA
                     SLGTARSFTLAGFSGHVFHNVNFVVLDDSAAPALVGQNGAASAAAVLGQNVLGLGDVE
                     YDLANGYIRLFKPENCADHMLAYWHGDKPVGVIKIAHINPVWTHLVGTATLDGSKINV
                     MFDSGATRSMLALKAARRAGFKPDNPDVTAAGISVGIGRRRIESWIARFDSLDLGGEE
                     VKNARLRVSDIDLANGVDMLLGADFFLSHRLYVANSQGRIFFTYNGGRVFDLGVADQG
                     QTRTAQQSDQAPATTSADTDASNPSAGQSEGGGTTSSSSATGTDAAQSGSEGAPANGS
                     LDAASLRRRGAASAGRLDYRAALADFDEAIRLEPNDAENYLQRATARAHEHETALALS
                     DYDQALRISPDNLSALLGRGELRLADRNESGARADFERASAIAATDPGPSLHIAEIYA
                     TTGHFTEAIALYDRWMAAHPADSRLAAAASGRCWARAATGKDLELALADCQLSVRRGG
                     ATSYTLQHRGLVWLRLGRFDEAIADYKASLKLQPRDPTARYGLGLAERGKGMQAQGDR
                     DIEAAKSESASVAALFKRIGLGS"
     gene            complement(3576..>3944)
                     /locus_tag="VFB37_05900"
     CDS             complement(3576..>3944)
                     /locus_tag="VFB37_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO22021.1"
                     /translation="QRNARLTRDRALFIARAWSRAAGEGPSEELRLTFDPRHRLVNPV
                     LYRREEAEACWSRITIPVLLIAGAESEHAERAERAGRRFSDLCRNLRMVTLSGVGHMM
                     HHEDPAAVAAAILEFEQANT"
BASE COUNT          693 a         1251 c         1293 g          707 t
ORIGIN      
        1 agcagttgca caccctggac cgcgcgggag cggggtgccc tggggttgcg gtaccatcgg
       61 tacaagaggg agtaccggag ctcatgcgcg gcaagggcaa aggcatcgcg tcgacgctcg
      121 tggccgcagc ggcagggatc agcgtggccg catgcggggg cggagctggc gccacgcagg
      181 ctgcgagcag cccgaccgtc accagcgagg ccgacacaat cctgcacatg gaccaggtgc
      241 gtgctgccgg ctacacgggc gccggcgtgc gggtcggcgt gatcagcact ggcgtggtga
      301 atttagcctc ctatcagacg gccggtgcct tacccacttc tttgtatgtc acgcagaatg
      361 ccgccggcat gctcgacgag ggaagctgga tgctcgagct cgtgcatcag cacgcgcccg
      421 atgccgtgct cggcttctgc gatggtctcg acctcgactt cgacggttgc atcaaggacc
      481 tggcgaataa tttccacgcc gacgtcatcg tcgatgacat cctgttctcc gggcagttct
      541 atccagatgc caccgccgac ctcgtcactc aactcgaggc tgccaacgac cggctcgttt
      601 tcatccatct tgccgggaat gagcagaaag gtggttactg gcaggggccc ttcattccga
      661 ccgaagcagt gctgaacggt gcgaccgaga cggtgctgga cttcggactt gcgagcgggg
      721 ccgccagcga tccgttcaac tcgatcagtg tgccggccgg tgcacatctg atcctgatca
      781 tgaactggaa tgacccgcct catggcacgg ccaatcatgc cttgagcgtt catctgctgg
      841 gagatgtgtc gcaggagttg gcccatgcga gcgggcagtc ggaccccacc gtgcgcctgg
      901 attacgtgaa cacgggcgcc agcgcgcaga ccgtgcggct ggctgtgtca ctcgatgagg
      961 gcacgcccgg tggcctggcg attcaggtca ccgagggtag ccccacgtgc aacatagagt
     1021 gccagccgat ggctcatgag agcgccgggc tggcgggtgg aacgatcggg gattttcccg
     1081 atgcgctggt cgtcggcgcg acgagcgcaa tgtcgccgcg ccagctcgag gcctggacga
     1141 atcgtggacc ttttcggatc gacttccagt ccacttccga ctcggcggca cccgatggct
     1201 acgactacgc gcggctggcc acgccgctca ggatcagcaa gccggatctc gtggcgcccg
     1261 attgcgtgac cgttcctttc agcagcggca ccgcgctgag caacgatacg ttctgcggca
     1321 catctgcagc agttccagca atcgccggtg ccgccgcgct gctcgaaagt gccggcttca
     1381 accggtctcg ggtgctgagg gcgttgcgga ccactgccgt gcgactcggc gcctctgcat
     1441 gggatccggg gtatggattc ggtctggccg atgccgccgc ggcgtggaga tccggcggct
     1501 gaacgaaccg gcgcatgaat tgaggcgctg actggcgggg ggctacccga tggaacggtt
     1561 catccgcgca cccggcaagc tgctggccgc ttgtctgctg tctttgctgc tgccgctcgt
     1621 ggcgagcgcg ggcggggaat gcagggtcgt ccgttttgcc gagttccctg tcacgatgtc
     1681 aggcacgcga ccgctgattg cgggctcgat caacggcgtg gatgcgctct tcatggccga
     1741 tagtggtgca tttttcagcg tgatctcgca cgagcgcgcc gagagctaca agctcctgat
     1801 gggtacgctg cccagcggac tggttgtgag cggcatcggc ggcacggcag gcgcgagtct
     1861 gggtacggcg agatctttca ccctggccgg attttcggga cacgttttcc acaacgtcaa
     1921 ctttgtcgtt ctggatgact cggccgctcc cgctctcgtc ggacagaacg gcgccgcatc
     1981 tgcggcggcg gtcctcggcc agaacgtgct cgggttggga gatgtcgagt acgacctcgc
     2041 caatggctat atccgtctgt tcaagcccga gaactgcgct gatcacatgc tcgcctactg
     2101 gcatggggat aagccggttg gcgtgatcaa gatcgcgcac atcaatccag tgtggactca
     2161 tctggtgggc acggcaacgc tcgacggctc gaagatcaat gtcatgtttg acagtggggc
     2221 gacgcgatcc atgcttgcac tcaaagctgc gcgtcgcgcc gggttcaagc cggacaatcc
     2281 ggacgtgacc gctgccggca tttcggtcgg catcggcagg cgccggatcg agagctggat
     2341 cgcacgcttc gacagtctcg acctgggcgg cgaggaagtg aagaacgccc gtctcagggt
     2401 cagcgacatc gatctggcca atggcgtgga catgctgctc ggcgcggact tctttctctc
     2461 tcatcggctc tacgttgcca acagtcaggg gaggatattt tttacttaca acggcggtcg
     2521 agtttttgac ctcggggtcg cggatcaagg acagacccgt acggctcaac agtccgatca
     2581 ggctccggcg accacatctg ccgataccga cgcctcgaat ccctcagccg gacagtctga
     2641 gggcggcgga acaacgagct catcgagcgc gaccggcaca gatgctgcac agtccggcag
     2701 cgagggcgcg ccagcgaatg gctcgctgga tgccgcttcc ctcaggcgcc ggggcgcagc
     2761 ctccgccggg cgcctggatt atcgcgccgc ccttgccgac ttcgatgagg cgatccgcct
     2821 ggagcccaac gacgcggaga actatcttca gcgcgccaca gccagagcgc acgagcacga
     2881 gacggctctt gcgctcagtg actacgacca agctctgcgg atcagtcctg acaacctctc
     2941 cgcgcttctc ggtcggggtg agctgcgcct ggctgacagg aacgaaagcg gggctcgggc
     3001 ggacttcgag cgtgcaagcg caatcgcggc gaccgatccc ggtccgagtt tgcacatcgc
     3061 agagatctac gccacgaccg ggcacttcac ggaggccatt gcgctttatg accgctggat
     3121 ggcggcgcat cctgcggaca gccggcttgc cgcggcggcg agcggtcgct gctgggcacg
     3181 cgcggcaaca ggcaaggact tggaactcgc cctcgccgac tgccagctca gtgtgcggcg
     3241 cggtggagcg acttcctata ccctgcagca caggggcctg gtgtggctgc gcctcggccg
     3301 tttcgatgag gcgatcgccg actacaaggc ctcactcaag ctgcaaccga gagatccaac
     3361 cgcgcgctac ggtctcggac tggccgagcg cggcaagggc atgcaggcgc agggtgatcg
     3421 ggacatcgaa gctgcgaagt cagaatctgc gagcgtagcc gccctcttca agcgcatcgg
     3481 gctcgggtcc tgaagcgccc gctcagccct tcagacgcag aggcaaacct gcggtgagct
     3541 cttcccgctg cgagcgccac cgatgagcag cgatctcacg tgtttgcctg ctcgaactcg
     3601 aggatcgcag cggccaccgc cgccggatcc tcgtgatgca tcatgtgccc gacacccgac
     3661 agggtgacca tccgcagatt gcgacaaaga tcgctgaagc gccgaccggc gcgctccgct
     3721 cgctctgcgt gctcggattc ggcgccggcg atcagaagca cgggaatcgt gatacggctc
     3781 caacaggcct cggcctcttc tctgcggtac aacaccggat tgaccaaccg atggcgcgga
     3841 tcgaaagtca ggcgcagctc ctcactggga ccttcaccgg ccgcgcgact ccaggcgcgc
     3901 gcgataaaca gtgcccgatc acgtgtcagt cgcgcgttac gctg
//
LOCUS       DASRHN010000154         8593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2194789, whole genome shotgun sequence.
ACCESSION   DASRHN010000154 DASRHN010000000
VERSION     DASRHN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194789"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..648)
                     /locus_tag="VFB37_05905"
     CDS             complement(196..648)
                     /locus_tag="VFB37_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HZO22022.1"
                     /translation="MSSSNVGQVLRASAFSYMVSLRARHPASNLAVLTETLRLEPAHS
                     WTAGEPRRSQSGVPLGGYHRDSYWAAPLPASLVGSSSMPLEGFLSQQLVQLARHRECL
                     KRLQADGGEVSLLIELSPVANAGFTLSSRIARQLADLNVEVEFQFAAD"
     gene            1074..3173
                     /locus_tag="VFB37_05910"
     CDS             1074..3173
                     /locus_tag="VFB37_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO22023.1"
                     /translation="MTSSRPEPVGTVEVALAHAGRLLARDPQLAATQAREILVVVPGH
                     PQARLLLARAERLGGHTRSAIEILQLLSREQPQLAAVHLELGLALGEGDRLPEAIEAF
                     RAALERSPQSADAWRLLADHLDALGDSGAADAARARHLKAVTRDPRLMRAAAALVEND
                     LPVAESALRAHLRTAPTDVAALRMLAEVAARLRRYADAQVLLERCLELSPSFSAARHN
                     YATVLNRQARPAEALPHIERLLQAEPRNPAYRNLKAATLANLGEYAQSIEVYESVLRE
                     FPRQPRVWMSYGHSLKTAGRRDESIAAYRRASEMEPTLGEAYWSLANLKTFRFADEDV
                     ATLRRALAGGELSDDDRLHFEFALGKALEDRAAHSEAFEHYSAGNAIRRKLHPYSADE
                     NAGYVRRSQALYTRRFFEARAGAGASAPDPIFIVGLPRAGSTLIEQILASHSHVEGTM
                     ELAELPRIARELAGPDPRQEEARYPQGVSRLNPAQLRGLGERYLAETRIHRKTDAPFF
                     IDKMPNNWLHAGLIHLILPQARIIDARRHPLGCCLSGFKQHFARGQAFAYDLRDIGLY
                     YRDYVQLLAHFDAVLPGRVHRVFHERIIDDTEAEVRRLLAYCGLPFEEACLRFYENTR
                     AVRTASSEQVRQPIFRDGLDQWRHFEPWLDPLKEALGPVLEHYPQVPAFAPLDRAERV
                     DGLASSSNKSDDRGESS"
     gene            3170..5845
                     /locus_tag="VFB37_05915"
     CDS             3170..5845
                     /locus_tag="VFB37_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22024.1"
                     /translation="MSRSRKSTTARKRLFAPAVTRGRPTWHAQPIATAVSAILAGIPL
                     AYAQEQATSQEPGTTGLSEVIVTAQKVVENVQNVPISIQTLNTEKLEQLNIVNIDDYV
                     KYLPGVTTVKGLGQGGTGIGTTHMYMRGVVSGQDGNHSASQPSVGTYFDEQPVTTIDG
                     TIDIHVYDIARIEVLEGPQGTLYGASSEAGTIRIITNKPDPSRFSAGYDLTGDAVDHG
                     HAGSVVEGYVNLPLTPAAAVRLVAWDEHDPGYIQNVAGTNAAAGIVNGQRTFPTWSAA
                     TGQTLSNESSLDSAYNTVSIKGGRAALKLNLGDNWTFTPTFMGQDTNSNGNFGYDPAV
                     GDLQIVHFGPEGNHDSFTQSALTVEGKISDFDLVYAGGWLVRNTHSIADYSDYSYFYD
                     KYFGSGCNWLTESGYQYKQSHPGAACVTGFPAGSYAEPQEFVITKGHYTKWSNEIRLS
                     TPQQLPVKGIVGLFAQRQVHEIWEQYVIPGLGGDPYSTNPQGFAPSLNIPGVTGNTIW
                     LTDEERVDRDQAAFGQAVWDITGSWELTGGIRFYKYDNSLQGFYGYSQAYQDLTGYYP
                     GQHICGPAGVGGQPNFQPFHGAPCTDLNAQVSDNGKTYRGTITYKLDPDRLLYATYST
                     GFRPGGVNRVFDPEINAVFPPYKADFLKNYELGWKTQWDGHRLRWNGAVFWENWDNFQ
                     FLYLGPNSVTVVQNAASAQIKGFETELEWLPVDSLLLSGGATYLHAVTTANYCGPAAT
                     IPGTTELSTNCPNQINSYAVGNAPNGPEAPAGTPLPVAPKVKVNLVARYTYPIVADWQ
                     GFLQGSGMYQTATEPLLRLIDQHTLGKMPPYALFDLSTGMNKENLSLQLTVTNLFDKR
                     AQLTRYVECATTTCNQPYVIPAQPRTIAIKLEQKF"
     gene            5859..7952
                     /locus_tag="VFB37_05920"
     CDS             5859..7952
                     /locus_tag="VFB37_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO22025.1"
                     /translation="MNATREPVGNVEVALAHAARLLGSQPELAAEQSTEILKAVPGHP
                     VAALILGRARRLTGDPEGALEVLEPLAQTQPTAAPVHLELGLALSAVGRNLESIPALR
                     RALKLKPDMPDAWRTLGDQLTLTGDAAGADAAYAQHIKASVHDPRLLVAAAALCENRI
                     PEAEALLREHLKQYPTDVAAIRMFAEVAARLQRYQDAEKLLVRCLELAPSFAPARHNY
                     AVVLHRQERSVEALREIESLLARDAHNPGYQNLKAAILAKLGELAQSLELYEGVLAAY
                     PRQPKLWMSYGHALKTEGREADCIAAYEKSIELQPGLGEAYWSLANLKTVRFTPRQLE
                     SMRQQLARSDLSDDDRLHLQFAMGKALEDAGDYAASFEHYAEGNRIRRAVVRYSADEM
                     SELVRRSKALFTPAFFAERLGHGSDRRDPIFIVGLPRAGSTLLEQILASHSQVEGTME
                     LPDIPGIAKSFAQPADRGATPRYPEALAGLSAAQCRALGERYLQQTRIQRKTAAPFFI
                     DKMPNNFMHVGLIQLILPQARIIDARRHPLGCCFSGFKQHFARGQHFSYSQEDIGRYY
                     SDYVELMAHFETVLPGRIHRVLYESLIEDTEAEVRSLLEYCGLPFESDCLRFYANERA
                     VRTASAQQVRRPIYREGIDHWRNFEPWLGPLKAALGPVLERYPQVPDFTATGAPARAR
                     PQQAHEHDKGGSSDT"
     gene            7942..>8593
                     /locus_tag="VFB37_05925"
     CDS             7942..>8593
                     /locus_tag="VFB37_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZO22026.1"
                     /translation="MTRSRSRKLKRLQGRKAKQMLWKAMPVASAILACMQTARAQAPS
                     DTEAPSGSGLEEVVVTAQKRVENMQNVPISIQAIGTEKLDQMHVTNFDDYAKLLPSVS
                     FQTAGPSFEHTYMRGVASGGDGNHSGSLPSVGMYLDEQPVTTIDGNLNIHIYDIARVE
                     ALSGPQGTLYGASSEAGTIRIITNKPDPTGFKAGYDVEGNSVDHGGKGYTVEGFVNM"
BASE COUNT         1623 a         2869 c         2699 g         1402 t
ORIGIN      
        1 agccgccatc gcccgacatg agcagcgcga agtgcttgac ctcaccctga ggccgaaaga
       61 cctccacgtg cttgaagcgt ccatgcgaaa gcgagtccgc gccggccgct gtgccggcgc
      121 cgagtgtggc cacgagcgcg aaggctgtaa gggcggcagt agcgcgcact acggcgaacc
      181 gcccggcaca cgcgctcaat ctgctgcgaa ctggaattcc acttcgacgt tcaggtccgc
      241 cagctggcgc gcgatgcggc tgctgagggt gaagccggca ttggctaccg gggaaagctc
      301 gatgagcagc gagacctcgc caccatcggc ctgcagccgc ttgaggcact cgcgatggcg
      361 tgcaagctgc acgagctgct ggctcaggaa cccttcgagc ggcatgctgc tcgagcccac
      421 gagcgatgcg ggcagaggcg ccgcccagta ggagtcccga tgatagcccc caaggggcac
      481 cccgctctgc gagcgacgcg gctctccggc agtccagctg tgcgcaggct ccaggcgcag
      541 cgtttcggtc aacaccgcca ggttcgatgc tggatgccgc gcgcgcagtg aaaccatgta
      601 gctgaaggcg ctggcccgca gaacctgacc gacattgctc gagctcattg gacctcacct
      661 ctccatcgct aaataccggt ccttcgatcg actcgcggcg ccactcgcgc aattgcaagc
      721 gatcggcggg agaataaccc ccggagcgct gcgctgtctt gactccagga tgccttgatc
      781 tggagcgaag ccaatacggc cagccgcgct gcgcgaggaa ccaacatcgt gtgcaagcag
      841 cgcgcggcgg gggctggcaa gcaaacgcgc gcgcgggcgc gatgagctcg ccatcgtatc
      901 cgtcgcgagt cgccgcagcc gacggtcggc ttgccgcaac agcttgacgc aaccggtgcg
      961 gattcctacc cttgaacccg tcaacacccg cgcgccttcg ttgcgcgtga gctacaaggt
     1021 gtctacgctg gcatggccac tgtattgagg cgcattgcgc gcagtgtcct cccatgacga
     1081 gcagccgccc ggagccggtg ggtaccgtcg aggtcgcact cgcgcacgcc ggccgccttc
     1141 tggcacgcga tcctcagctg gcggcaaccc aggcgcgcga aatactggtc gtcgtcccgg
     1201 gccatccgca agcgcgcctg ctgctcgccc gggccgagcg gttgggcggg catacccgct
     1261 cggcgatcga gattctccag ctgctgtcgc gggagcagcc ccagctcgcc gccgtccatc
     1321 tcgagctggg attggctctg ggcgaaggcg accgcctgcc cgaggcaatc gaggcctttc
     1381 gcgcggcgct cgagcgcagc ccgcaatccg cggacgcctg gcggctgctc gccgaccatc
     1441 tggatgctct cggggatagc ggtgcggcgg atgccgctcg cgcccgccac ctcaaagcag
     1501 tcacccggga cccgcgattg atgcgcgcgg ccgccgcgtt ggtggagaat gatctcccgg
     1561 tggccgaatc ggccctgcgc gcacatctgc gcaccgcacc gacggacgtg gcggccctgc
     1621 gcatgttggc cgaagtcgct gcgcgactgc gacgctacgc agatgcgcag gttctcctgg
     1681 aacgctgcct ggagctctca ccgagcttca gcgccgcccg ccacaactac gccaccgtac
     1741 tcaatcgcca ggcccggccc gccgaggccc tcccccacat cgaacgcttg ctgcaggcgg
     1801 aaccgcgcaa cccggcctat cgaaatctca aggccgctac tcttgccaat ctcggcgagt
     1861 acgcgcagtc gatcgaggtg tatgagtccg tgctgcggga gttcccccga cagccgcgcg
     1921 tgtggatgag ctacggccac tccctgaaaa cggccggtcg tcgtgatgag agcatcgctg
     1981 cataccggcg cgcgagtgag atggagccca ccctgggcga ggcgtactgg agtctcgcga
     2041 acctcaagac cttccggttt gcggatgagg atgtggcaac cctccggcgc gcgctcgcgg
     2101 ggggtgagct ttcggacgat gaccgactgc acttcgaatt cgcgctgggc aaagcgctcg
     2161 aggatcgggc agcgcactcg gaggcttttg aacattacag cgcgggcaac gcaatccggc
     2221 gcaagctgca tccctacagt gcagacgaaa acgcaggcta cgtgcggcgc tcgcaggcac
     2281 tgtatacacg ccgcttcttc gaagcgcgtg ccggggccgg cgcgagcgca ccggatccga
     2341 tcttcatcgt ggggctgccg cgcgcgggct cgacgctgat cgagcagatc ctggccagcc
     2401 actcccacgt cgaggggacg atggagctcg ccgagcttcc acggatcgcc cgtgagctcg
     2461 caggtccgga cccgcggcaa gaggaggctc gctatccgca gggggtgagc cggctgaatc
     2521 cggcacagtt gcgcgggctc ggtgagcgtt atctcgccga gacacgaatt catcgcaaga
     2581 cggacgctcc gttcttcatc gacaagatgc ccaacaactg gctgcacgca gggctcattc
     2641 acctgatcct gccgcaagcc cgcatcatcg atgcccgtcg ccaccccctc ggatgttgcc
     2701 tttccggctt caagcagcac tttgcccggg gtcaggcctt tgcctatgac ctgcgcgaca
     2761 tcgggttgta ttaccgcgac tatgtgcaac tcctggcaca cttcgatgcc gtcctgccgg
     2821 gcagggtgca tcgcgtattc cacgagcgga taatcgacga caccgaagca gaggtgcgca
     2881 gactgctcgc ctactgcggg ctgcccttcg aggaggcgtg cctacgtttc tacgagaaca
     2941 cccgggcggt gcgcacggcg agctccgagc aggtgcggca gcccatcttt cgcgacggcc
     3001 tggatcagtg gcggcatttc gagccgtggc tcgatcctct gaaggaggcg ctcgggccgg
     3061 tgctcgagca ctatccgcag gtgcccgcct ttgccccact ggaccgtgcc gaaagagtcg
     3121 acggcctcgc atcatccagc aataaatctg acgatcgggg agagagctca tgagtcgtag
     3181 ccgaaagagc accacggcgc gcaagcgcct ctttgcgccg gccgttacgc gcggccggcc
     3241 gacctggcac gcacagccga tcgccacggc cgtatcggcc attctcgctg gaattccgct
     3301 ggcgtacgca caggaacagg ccacatcgca ggagcccggc acgaccgggc tctccgaggt
     3361 catcgtgacc gcgcagaagg tcgtggagaa cgtccagaac gtgccgatca gcatccagac
     3421 tctcaacacc gagaagctcg agcagctgaa catcgtcaac atcgacgact acgtgaagta
     3481 cctgccgggc gtcaccaccg tcaaaggcct cggccagggt ggcaccggca tcggcacgac
     3541 acacatgtac atgcgcggcg tggtcagcgg tcaggacggc aaccactcgg catcgcagcc
     3601 cagcgtcggg acctacttcg atgagcagcc cgtcacgacc atcgatggga cgatcgatat
     3661 ccacgtatac gacattgcgc gcatcgaggt gctggaaggc cctcaaggga cactgtacgg
     3721 cgcaagctcg gaagccggca cgattcggat catcaccaac aagccggatc cgtccagatt
     3781 ctccgccggc tacgatctca cgggcgacgc ggtggatcac gggcatgccg gttccgtggt
     3841 cgagggctac gtcaacctgc cgctgacccc cgctgctgca gtacgcctcg ttgcctggga
     3901 tgagcacgat cccggctaca tccagaatgt cgccgggacc aacgccgccg cggggatagt
     3961 caatgggcag cgcaccttcc cgacctggag tgccgccacg ggccagacgc tcagcaacga
     4021 gagctcgctc gacagcgcct acaacaccgt gtcgatcaaa ggcggacgcg cggcactgaa
     4081 gttgaatctc ggcgacaact ggaccttcac tccgaccttc atgggccagg ataccaactc
     4141 aaacggcaac ttcggctacg acccggcggt cggcgacctg cagatcgtgc atttcggacc
     4201 ggagggaaat catgactcct tcacgcagag cgcgctgacc gtcgagggca agatcagcga
     4261 cttcgacctg gtctacgccg gcggctggct ggtgcgcaac acccactcga tcgcggacta
     4321 cagcgactac tcctacttct acgacaagta cttcggctcg ggctgcaact ggctcaccga
     4381 gagcggctat cagtacaagc agagccatcc gggcgcggcg tgcgtcaccg gctttccggc
     4441 cggctcctat gccgagccgc aggaattcgt gatcacgaag ggccactaca ccaagtggag
     4501 caacgagatc cgtctgagca cgccgcaaca gctgccggtg aaaggcatcg tgggcctgtt
     4561 cgcccagcgc caggtgcacg agatctggga gcagtacgtc atcccgggcc tcggcggcga
     4621 cccctacagc acgaatccgc agggctttgc gccgagcctc aacattcccg gcgtcaccgg
     4681 caatacgatc tggctcacgg acgaggagcg tgtggaccgc gaccaggccg cgttcggcca
     4741 ggcggtctgg gacatcaccg gaagctggga gctcaccggc ggcattcgct tctataagta
     4801 cgacaactca ctgcagggct tctacggtta ctcccaggcc taccaggatc tcaccggcta
     4861 ctatcccggc cagcatatct gcgggcctgc gggcgtgggc ggccagccga actttcagcc
     4921 cttccacggc gctccgtgca ccgatctcaa cgcgcaggtc tcggacaacg gcaagaccta
     4981 tcgcggcacc atcacctaca aactcgatcc ggatcggctc ctgtacgcga cgtactcgac
     5041 cggttttcgc cccggcggcg tgaaccgcgt gttcgacccg gaaatcaacg ccgtcttccc
     5101 gccctacaaa gccgacttcc tgaagaacta tgaattgggc tggaagacgc agtgggatgg
     5161 acaccggctg cggtggaatg gcgccgtgtt ctgggagaac tgggacaact tccagttcct
     5221 gtatctgggc ccgaacagcg tgacggtcgt gcagaacgct gcctccgccc agatcaaggg
     5281 attcgagacc gagctcgaat ggctgccggt ggacagtctg ctgctgtccg gaggcgcaac
     5341 ctacctgcat gccgtcacga ccgccaacta ctgcggtccg gcggccacca ttcccggcac
     5401 gactgagctg tcgaccaact gcccgaacca gatcaactcc tatgcggtcg ggaatgcgcc
     5461 caacggcccc gaagccccgg ctggcacgcc gctcccggtc gccccgaagg taaaagtgaa
     5521 tctcgtggcg cgctacacct atcccatcgt ggcggactgg cagggatttt tgcagggatc
     5581 gggcatgtac cagacggcca cggagccgct gctgcggttg atcgaccagc acaccctcgg
     5641 caagatgccc ccgtacgccc tgttcgatct gtccaccggg atgaacaagg agaacctgtc
     5701 gttgcaattg accgtcacca atctctttga caagcgtgcg cagctgactc gctacgtgga
     5761 gtgcgcgacg acgacctgca accagcccta cgtcattccg gcccagccgc gtacgatcgc
     5821 gatcaaattg gagcagaagt tctgagcgaa aggatctggt gaacgcaacg agagaaccgg
     5881 taggcaatgt tgaggtcgcg ctggcccatg cagcgcgact gctggggagt cagccggagc
     5941 ttgccgccga acaatccacg gaaatattga aggccgtccc gggccatccg gtggcggccc
     6001 tcatcctcgg acgcgcccgc cggctgaccg gcgatccaga aggcgccctg gaagtgctgg
     6061 agccgcttgc gcagactcag cccaccgccg cgcccgtgca tctggaattg gggctcgcgc
     6121 tgagcgccgt ggggcgcaac ctcgagtcga tccccgccct gcgtcgcgcc cttaagctca
     6181 aacccgacat gcccgatgcg tggcgtacgc tgggcgatca actcacgctc acaggtgatg
     6241 cggccggcgc cgatgccgcc tatgcccagc acatcaaagc ctcggtgcac gatccgcgtc
     6301 tgctggtcgc cgccgcagcg ctctgtgaga accggattcc cgaggcggag gcgctcctgc
     6361 gcgaacacct gaagcaatac ccgacagatg ttgccgcgat acgcatgttc gccgaggtcg
     6421 ccgcccgtct gcagcgctac caggatgcgg agaaactgct cgtgcgctgc ctcgagctgg
     6481 cgccgagttt cgcccccgcg cgccacaact acgccgtcgt actgcacagg caggaaaggt
     6541 ccgtggaggc gctgcgcgag atcgaatccc tgctggcgcg ggatgcgcac aatccggggt
     6601 accagaacct gaaggccgcg atcctcgcca agctcggcga gctcgcgcag tcactggagc
     6661 tgtacgaggg agtgttggcc gcttatcccc ggcagccgaa gctctggatg agctacggac
     6721 acgcgctgaa gaccgagggt cgcgaggccg actgtatcgc ggcctacgag aagagcatcg
     6781 agttgcagcc gggtctcggc gaggcgtact ggagccttgc caatcttaag acggtacgct
     6841 tcacacccag gcaactggaa tcgatgcgcc agcagcttgc caggagcgat ttgagcgacg
     6901 acgaccgcct gcatctgcag tttgcgatgg gcaaggcgct ggaggatgcc ggtgactatg
     6961 ccgcctcgtt cgagcattac gccgagggta atcgcatccg ccgcgcggtg gtccgctatt
     7021 cggctgatga gatgtcggag ctcgtccgcc gcagcaaagc cctgttcacg cccgccttct
     7081 tcgccgagcg cctcggccat ggcagcgacc gcagggaccc gatcttcatc gtgggtctgc
     7141 cgcgtgccgg ctcgacgctg ctcgagcaga tcctggccag ccattcgcag gtggagggca
     7201 cgatggagct gccggatatt cctggcatcg cgaaatcatt tgcccagcca gccgaccggg
     7261 gcgcaacccc gaggtatcca gaggcgctcg ccggtctgag tgctgcgcag tgtcgcgctt
     7321 tgggcgagcg atatctgcag caaacccgca tccagcgcaa gacggcggcg ccgttcttca
     7381 tcgataagat gccgaacaat ttcatgcacg ttggcctcat ccagctcatc ctgccccagg
     7441 cccgcatcat cgacgcacgc cgccacccgc tcggctgctg tttctccggc ttcaagcagc
     7501 actttgcccg ggggcagcat ttcagttaca gccaggagga tatcggccgg tattacagcg
     7561 actacgtcga gcttatggca cacttcgaga ctgtgctgcc aggtcggatt caccgtgttc
     7621 tctacgaatc gctgatcgag gacacggagg ccgaggtgcg tagcctgctg gaatattgcg
     7681 gcctgccgtt cgaatcggat tgcctgcggt tctacgcgaa cgagcgggcg gtgcgcacgg
     7741 cgagcgcgca gcaggtacgt cggccgattt accgggaagg catcgatcac tggcggaact
     7801 tcgagccgtg gctcggtccg ctcaaggctg ctctgggccc ggtgctcgag cgctacccgc
     7861 aggttccgga ttttacggcg accggggctc ccgcaagggc ccggccgcag caagcacatg
     7921 agcatgacaa ggggggttcc agtgacacgt agccgatcgc gaaaactcaa gcgcctgcaa
     7981 ggtcgcaagg cgaagcagat gctgtggaag gcgatgcctg tggcttcggc catactcgcc
     8041 tgcatgcaaa ccgcgcgcgc ccaggcgcca agcgacaccg aagcgccctc cggctccggc
     8101 ctggaagagg tggtggtcac cgcgcaaaag cgcgtcgaga acatgcagaa cgtgccgatc
     8161 agcatccagg cgatcggcac cgagaagctg gatcagatgc acgtgaccaa cttcgacgat
     8221 tacgcgaagt tgctccccag cgtctcgttc cagaccgccg gcccctcgtt cgagcatacc
     8281 tatatgcgcg gcgtcgccag cggcggcgac ggcaatcact cgggctccct cccgagcgtg
     8341 ggcatgtacc tggatgagca gccggtcacg acgatcgatg gcaacctgaa catccatatc
     8401 tatgacattg cgcgcgtgga ggcgctgtcc ggaccgcagg gcacgctgta cggcgcaagc
     8461 tccgaggcgg gcacgattcg catcatcact aacaagcccg acccgaccgg cttcaaggcc
     8521 ggctatgacg tcgaaggtaa ctcggtagac cacggcggga agggctacac ggtcgaggga
     8581 ttcgtgaata tgc
//
LOCUS       DASRHN010000155         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_543814, whole genome shotgun sequence.
ACCESSION   DASRHN010000155 DASRHN010000000
VERSION     DASRHN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543814"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFB37_05930"
     CDS             complement(<1..349)
                     /locus_tag="VFB37_05930"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZO22027.1"
                     /translation="MIFEDEPVPGYPLPILPGHTSPGRFERVLRAGQFAITSELAPPD
                     SADPEEVYQRARMFDRYVDAINATDGSGANCHMSSLAVCALLTRVGYAPVMQISCRDR
                     NRIAIQGDILGGAA"
     gene            complement(346..804)
                     /locus_tag="VFB37_05935"
     CDS             complement(346..804)
                     /locus_tag="VFB37_05935"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZO22028.1"
                     /translation="MYAMRRWSVRHARGLNAFYRSFEAVICWLHPLWKRIGYARLERP
                     VAAVERNVKGFLFDCRMCGQCVLSSTGMSCPMNCPKNLRNGPCGGVRSDGRCEVRPDM
                     KCVWHEAVAGSARIPGGAEALRTLQPAIDRTLQGRSSWLRVARLRAGEPG"
     gene            complement(804..1112)
                     /locus_tag="VFB37_05940"
     CDS             complement(804..1112)
                     /locus_tag="VFB37_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HZO22029.1"
                     /translation="MATLILISWRDIPAQVIAKRGRETAKVQLSARFQEAVDRAAMRA
                     GKGSSDAYLADWKRSEPEPCAEDIQQVVAERAAQIEASYTDEDLERLIKAKGLAGADG
                     "
     gene            complement(1100..1717)
                     /locus_tag="VFB37_05945"
     CDS             complement(1100..1717)
                     /locus_tag="VFB37_05945"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HZO22030.1"
                     /translation="MTRGILIIACGALAREITALKRANSWSEVDVQCLPAELHNRPER
                     IVPAVRAQISAHRNDYEQIFVAYADCGTGGMLDALLREEGIERLPGAHCYEFYAGSTT
                     FAELQDLEPGTFYLTDFLVRHFDRLVTRGLALDRHPELAREYFRHYRRLVYLCQRPNP
                     TAQQRAQQIAAELGLEFELRLTGYGELGSRLTALVATHREDSWPR"
     gene            complement(1730..2431)
                     /locus_tag="VFB37_05950"
     CDS             complement(1730..2431)
                     /locus_tag="VFB37_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HZO22031.1"
                     /translation="MAEQADAEFNLRDLNDQELIEQVHDDLYNGLKTEVEEATHIFLE
                     RGWGAEKVLNEALVEGMRIVGIDFRDGILFVPEVLLAANSMKGGMEILRPLLAETGVE
                     PIGKIVIGTVKGDIHDIGKNLVSMMLEGAGFEVIDLGINNPVEKYLAALEEHKPDILG
                     MSALLTTTMPYMKVVIDTMKEKGIRNDYIVLVGGAPLNEEFGKAVGADAYCRDAAIAV
                     ETAKGLLAARRAARV"
BASE COUNT          466 a          840 c          780 g          474 t
ORIGIN      
        1 tggcggcgcc gcccagaatg tcgccttgaa tggcgatccg gttgcggtcc cgacaggaga
       61 tctgcattac cggtgcatag cccacacgag tcaacaatgc gcagaccgcg aggctcgaca
      121 tgtggcagtt ggctccgctg ccgtccgtag cgttgatcgc atcgacatag cgatcaaaca
      181 tccgggcgcg ctgatagacc tcctcggggt ccgccgagtc cggcggtgcg agctcgctgg
      241 tgatggcgaa ctggccggcg cgcaggaccc tttcaaaacg gccgggcgag gtatgacctg
      301 gcaggatggg cagcggataa cccggtaccg gctcgtcttc gaatatcatc cgggctctcc
      361 cgctcgcagc cgggcgaccc gcagccagga agagcggccc tgcagcgtgc ggtcgatggc
      421 cggctgcagg gtgcgcagcg cctcagctcc gccagggatt ctggcgctgc ctgctaccgc
      481 ttcatgccag acgcatttca tgtcaggacg gacttcacaa cgaccgtcgc tgcgcactcc
      541 gccgcaagga ccattgcgca ggttcttggg gcagttcatg gggcaggaca tgccggtgga
      601 gctcagaaca cattgaccgc acatcctgca gtcgaagaga aaacccttca cgttccgctc
      661 aaccgccgcg accggacgct ccaggcgcgc ataaccgatt ctcttccaca gcggatgcaa
      721 ccaacagatc actgcctcga agctgcgata gaaggcattg agcccgcggg cgtgacgcac
      781 cgaccagcgg cgcatggcgt acatcagcca tccgccccgg caagaccctt tgccttgatg
      841 aggcgttcca gatcctcatc cgtataactc gcctcgatct gcgcggccct ctcggccacc
      901 acttgctgaa tatcctccgc gcagggctcc ggttcgcttc gcttccagtc agccaggtag
      961 gcatccgagc tgcccttgcc ggcgcgcatg gcggcgcgat cgacagcttc ctggaagcgc
     1021 gcggagagtt gcaccttggc cgtctcgcgg ccccgcttcg caatgacctg cgccggaatg
     1081 tcccgccagg aaatgaggat caacgtggcc atgagtcctc gcggtgagtc gcaacgagag
     1141 ccgtgaggcg cgagccgagc tctccataac cagtgagacg caattcaaac tccaggccga
     1201 gctcggcggc gatctgttgg gcgcgctgct gcgccgtcgg gttggggcgc tggcacagat
     1261 agacaagtcg gcgatagtgg cggaagtact cgcgggcgag ctcaggatga cgatcgagcg
     1321 cgaggccgcg ggtgaccagg cgatcgaagt gccgcaccag gaaatcagtc agatagaagg
     1381 tgccgggctc cagatcctgg agctcggcaa atgtcgtgga cccggcgtag aactcatagc
     1441 aatgcgcacc gggcagccgc tcgatgcctt cttcccgaag cagtgcatcg agcatcccac
     1501 ccgtcccgca gtccgcgtag gcgacaaaaa tctgttcgta gtcgttccgg tgagcgctga
     1561 tctgggcgcg cacggcaggc acgatgcgct ccggacggtt gtgcagttcc gccggcagac
     1621 actgaacatc gacctcactc cagctgttgg cgcgctttaa agccgtgatt tcgcgggcaa
     1681 gcgctccgca ggcgatgatg agaatgccac gcgtcacggc tctcttgcat cagacgcgcg
     1741 ccgctcgacg cgcggccagc aaccccttgg cggtttccac ggcaatcgcg gcgtcgcggc
     1801 aataagcgtc ggcgcccaca gccttgccga actcctcatt gaggggcgct ccgccgacca
     1861 gcacgatgta gtcgttgcgg atgcctttct ccttcatcgt gtcgatcacc accttcatgt
     1921 acggcatggt ggtcgtcagc agcgcggaca ttccgaggat gtcgggcttg tgctcctcga
     1981 gcgcggcgag gtatttctcc acggggttgt tgattccgag gtcgatgact tcgaacccgg
     2041 cgccctcgag catcatcgaa acgaggttct tgccgatgtc gtggatatcg cccttgaccg
     2101 tgccgatgac gatcttgccg atgggctcga ccccggtctc ggccagcagc ggccgcagaa
     2161 tctccatgcc gcctttcatc gagttcgcgg ccagcagcac ttcggggacg aacaagattc
     2221 cgtcacggaa gtcgatgccc acgatccgca tgccttccac cagtgcttcg ttcagcacct
     2281 tctcggcacc ccagccgcgc tccaggaaga tgtgggtcgc ttcctcgacc tcggtcttca
     2341 gaccgttgta gaggtcgtca tggacctgct cgatcagttc ctgatcgttc aggtcgcgta
     2401 aattgaactc ggcgtcggcc tgctctgcca tgctgtcctc atgcgctgcg cgcaacttct
     2461 cggcaggtcg aaggcgcggc tttcctttcc gactcccgac ctgcctgcac ttcggaacgg
     2521 aagctaccta gctggatcgc gaagcgcgac cccccaaaca
//
LOCUS       DASRHN010000156         6118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1700750, whole genome shotgun sequence.
ACCESSION   DASRHN010000156 DASRHN010000000
VERSION     DASRHN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700750"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFB37_05955"
     CDS             <1..589
                     /locus_tag="VFB37_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HZO22032.1"
                     /translation="NVRAFTEMQAREGLERPKVSLWLTGLKETIAQLPEFVRIAHAIG
                     VKEVYLQRLVFCEDAPVGMATPDQALFECLTRAEAASLAAAEAQAAALGISFNASGAT
                     EPGESLARKADTNPWSLCRRPWAVMYFTANGRALPCCIAPFSQNGYENYTLGDATQQT
                     LREIWNGRAYREFRAALQSDAPPKSCASCGLRWSL"
     gene            606..1337
                     /locus_tag="VFB37_05960"
     CDS             606..1337
                     /locus_tag="VFB37_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO22033.1"
                     /translation="MPIDRSKVSVVIPCLDEEAAIGPCVAAVLAHGVGEAIVVDGGSS
                     DRTAERALAAGARVIVEPRRGYGLALLTGLAAISPAAEAILFIDGDGSDRPEMIPDVL
                     APIERGDADFVLGSRLRGPRERGSLTPAQILAGHLAGLLIRLRYGVRFTDMSPFRAIR
                     RDALESLGMRETTFGWNLEMQMRAAAAGLRIVEVPVGQRRRVGGVSKVSGNLLTAARV
                     ACVLVATFLRLVARMPNPESALHRH"
     gene            complement(1374..2201)
                     /locus_tag="VFB37_05965"
     CDS             complement(1374..2201)
                     /locus_tag="VFB37_05965"
                     /inference="COORDINATES: protein motif:HMM:NF009165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO22034.1"
                     /translation="MHGECLTRVVSGQVRGSSPAATWLSDSRPSNAEWLGNPYARVMR
                     LARHGAGGQFRYATGIGLQPNRYFSRRPRPPYSEARRANLAEGKCMVGKLESCWSELM
                     RAAIRGDAAAYRQLLEELAPVLRRLVNRELHRCDSGEAEDVVQEALLALHLKRHTWDP
                     SRPFLPWVWAIARNKLTDALRRRPRQVHLPIDDMAEVLASDAVVDTDRVDAARLLAGL
                     KGRRREIVYSIFVEGASSREVAERLGMNEGTVRVALHRGVQAMARAFRGGMEVPTRS"
     gene            2196..2693
                     /locus_tag="VFB37_05970"
     CDS             2196..2693
                     /locus_tag="VFB37_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZO22035.1"
                     /translation="MHARIVPIVSAVAAALVSISLVAGSLAGRAENAASEGGVVRVKS
                     AYSFPETVERLKSNVASKGIMMFSEIDQARLAAGAGIALRPSTLLVFGNPPLGVQFIT
                     SRAEAGLDWPVRLLVYEDKSGQIWAAYSDFDWIARRHGITDREQAFKMASSVIASITA
                     SVASK"
     gene            2812..3510
                     /locus_tag="VFB37_05975"
     CDS             2812..3510
                     /locus_tag="VFB37_05975"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HZO22036.1"
                     /translation="MPARTIAEAFEHARSLDAPLAERLDTYAQSLREINQPMAEAVDR
                     LVARLNQSRMADSAPAVGEAMPAFLLPDHTGRLVGLEELLAQGPLAVTFARGHWCPYC
                     RIAVSALAEIADDVAGAGGAIVAIVPDRQEYAAKLRAEAHAPFPILTDIDNAYALSLG
                     LVFWVGEEMEHHMRARDVDLGSSQGNQSWFVPVPATFIVGTDGRIVARHVDPDYRKRM
                     EVEAVLTALRAARG"
     gene            complement(3517..4407)
                     /locus_tag="VFB37_05980"
     CDS             complement(3517..4407)
                     /locus_tag="VFB37_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZO22037.1"
                     /translation="MEMHQVRYFLTVARTLNFTRAAEQCNVSQPALTRAIRQLEEELG
                     GELLRREGKLSHLTELGHRMLPLMQQCYDSALAAKSLASSLKKGVASPLALGLSHTID
                     MRLLTPVLTELQRAVAGLQLKVLRGNAGEIAEALQKGRVEFAIAGPLEQTWDRLEAWP
                     LFDERFDLAIHRDHPLANAQAVDAGKLAEQRLLISEQCEQADSLAQILNERRIASVTN
                     HKLNSLDDVVALVAANLGVALLPQSAGHYAHLRRIAVADLDLARKVSLYGVAGRQRSA
                     GGDALMKLLRARAWADSPRA"
     gene            4777..5214
                     /locus_tag="VFB37_05985"
     CDS             4777..5214
                     /locus_tag="VFB37_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO22038.1"
                     /translation="MAVLLAPAAASAGACPAGKSVADGQGQKMSTAKAKGVTDKVQAR
                     IDLANEPVALKEHALRLRRLVIQPGGIVPWHSHTDRPAIIYIISGTITEYRSTCAVPI
                     VHRAGESTAEVKGTSHWWKNTGKTTVVLLSADLFHDKDDAHMM"
     gene            5332..>6118
                     /locus_tag="VFB37_05990"
     CDS             5332..>6118
                     /locus_tag="VFB37_05990"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HZO22039.1"
                     /translation="MPQATEEYAALPPARELTGKTAIVTGSTSGIGLGIVRAFAEAGM
                     HVMLNGFGDKAEIERTRMELEERFHIKTAYSAADMSKPTDIVGMVEDARRLLGKVDVL
                     VNNAGIQHVEAIETFPIPKWDAIIAINLSSAFHATRAVVPEMKARKWGRIINIASAHG
                     LVASPFKSAYVAAKHGLLGLTKTVALETAEHGITANAICPGYVLTPLVEKQIPETAKA
                     RGLTEEQVVKDVLLHAQPTRQFVTTGQVGALALFLCTDAAASMT"
BASE COUNT         1033 a         2069 c         1990 g         1026 t
ORIGIN      
        1 caacgtgcgc gccttcaccg agatgcaggc gcgggagggc ctcgagcggc ccaaggtgtc
       61 gctgtggctg accggcctca aggagacgat cgcgcagctt cccgagtttg tgcgcattgc
      121 ccacgcgatc ggcgtgaagg aagtctacct gcagcggctg gtgttctgcg aggacgcccc
      181 ggtgggcatg gccacccccg atcaggccct gttcgagtgc ctgacccggg cggaggcggc
      241 atccctcgcg gccgcggaag cgcaggccgc cgccctcggc ataagcttca atgcttccgg
      301 cgccacggag ccgggcgaga gtctggcgcg caaggccgac acgaatccct ggtcgctgtg
      361 tcgtcgccca tgggccgtca tgtatttcac cgccaacgga cgggcgctgc cctgctgcat
      421 cgcgccgttc tcccagaacg gctacgagaa ctatacgctc ggcgatgcga cgcagcagac
      481 cctgcgcgag atctggaacg gccgcgccta tcgggaattc cgggcggcgc tgcagtcgga
      541 cgcaccgccc aaatcctgcg cgagctgcgg tctgcgttgg agcctctagc gtgcccggtg
      601 cggccatgcc aatcgaccgc tcgaaggtct ccgtcgtcat cccttgcctc gatgaggagg
      661 ccgccatcgg cccctgcgtt gcagccgtgc tcgcgcacgg cgtcggtgag gcgatcgtgg
      721 tggacggcgg ctcgtccgac cgcacggcgg agcgggcgtt ggcggcgggc gcgcgcgtga
      781 tcgtggagcc caggcgcggc tacggcctgg ccctgctcac gggcctcgct gcgatctcgc
      841 ctgccgccga agccattctt ttcatcgacg gcgatggcag cgatcggccg gaaatgatcc
      901 cggacgtgct cgcgcccatc gagcggggcg atgccgattt cgttctgggg tcgcgcttgc
      961 gcggaccccg cgagcggggc agcctgacac ccgctcagat cctcgcaggc caccttgccg
     1021 ggctgctgat caggctgcgc tacggcgtcc gctttacgga tatgtccccg ttccgtgcca
     1081 tacggcgcga cgccctcgaa agcctcggca tgcgcgagac gacgttcggc tggaacctgg
     1141 aaatgcagat gcgcgcagcg gctgccggat tgcgcattgt cgaggttccg gtcggccaga
     1201 ggaggcgcgt cggcggcgtc tcgaaggtgt ccggcaatct cctgaccgct gcccgtgtcg
     1261 cctgtgtatt ggttgcaacg ttcctccgcc ttgtggcgcg aatgccaaat ccagagtctg
     1321 cgctgcatcg gcattgagcg tccggtcggc gacgcgcgag ggaagccggg ccgtcaactg
     1381 cgggttggaa cctccatgcc gccgcggaaa gctcgggcca ttgcctgcac gccgcggtgc
     1441 agggctacgc gcaccgtgcc ttcgttcatg cccaagcgct cggcgacttc gcgggagctc
     1501 gcgccctcga cgaagatcga atagacgatc tcgcgccttc tgcccttcaa gcccgccagc
     1561 agccgcgcgg cgtcgacccg atccgtgtcg acgacggcgt ccgaagcgag cacttcggcc
     1621 atgtcatcga tcggcaggtg cacttggcgc gggcgccggc gcaacgcatc cgtcagcttg
     1681 tttctggcga ttgcccacac ccagggcagg aacgggcggg acggatccca ggtgtggcgc
     1741 ttcaagtgca gagccagcag cgcctcctgg accacgtcct cggcctcgcc ggaatcgcat
     1801 cggtgcaatt ctctattcac caggcgccgc agcaccggtg cgagctcctc cagcagctgc
     1861 cgatacgcgg cggcatcgcc gcggattgcg gctcgcatga gctccgacca acaggattcc
     1921 agtttcccca ccatgcactt tccctcagcg agattggccc ttcgagcctc actgtaggga
     1981 ggtcgaggcc gccgggagaa ataccggttg ggttggagtc ctatgcctgt cgcgtatcga
     2041 aattgtccgc ccgccccatg cctggccagt cgcataaccc gagcgtatgg atttccaagc
     2101 cactcggcat ttgagggacg gctgtcgctc agccatgttg ctgccgggct ggaaccacgc
     2161 acctgacccg acacaactct cgtgaggcat tcgccatgca tgcgcgcatc gtccccatcg
     2221 tctcggctgt agccgccgcc ctggtttcga tctccctggt agccggatcg ttagccggtc
     2281 gcgcagagaa tgccgcctcg gagggcggcg tcgtgcgcgt caagagtgct tattcgttcc
     2341 ccgagacggt tgagcgtctc aagagcaacg tggcgtcgaa aggcatcatg atgttctcgg
     2401 agatcgatca ggcccggctc gcggccggcg ccggcatagc attgcgcccg tccacgctac
     2461 tcgtcttcgg caaccccccg ctcggtgttc agttcatcac gtccagggcc gaggccggcc
     2521 tcgactggcc cgtgcgcctg ctcgtctatg aggataagag cgggcagatc tgggccgcct
     2581 acagcgattt cgactggatc gcccgtcgcc acggcatcac ggaccgcgaa caggccttca
     2641 agatggcttc gagcgtgatc gcatcgatca cggccagcgt cgcatcgaaa tgaattcggc
     2701 cgggcgaatg gaaggctcga gtgggatgcg caagcacgca atcttgcgct cccgaagcgg
     2761 agatatatcc tgctcgaaat gtgcctgacc gtccgtaccg acgagagtgt gatgccagcc
     2821 agaaccatag ccgaagcgtt cgagcatgcg cgctctctcg atgccccgtt ggccgagcgg
     2881 ctggacacct atgcccagtc cctccgcgag atcaatcagc ccatggccga agccgtcgac
     2941 aggctggtgg cgcgcctcaa tcagagtcgg atggcggaca gcgcgccggc cgtgggtgag
     3001 gccatgccgg ccttcctgct cccggaccat acgggccgcc tggtcgggct cgaggagctg
     3061 ttggcacaag gacccctggc cgtcaccttc gcgcgtgggc attggtgccc ctactgcagg
     3121 atagcggtga gcgcactggc cgagattgcc gacgatgtgg caggagccgg aggggctatc
     3181 gttgccatcg tgccggaccg gcaggaatac gctgccaagc tgcgtgccga agcccatgcc
     3241 ccgttcccca ttctcaccga tatcgacaac gcttatgcgt tgtcgctcgg gctcgtgttc
     3301 tgggtcggcg aggagatgga gcatcacatg cgagcgcgcg acgtcgatct tggcagctcg
     3361 cagggcaacc agagctggtt cgtgcccgtg cctgcgacct tcattgtcgg cacggacggc
     3421 aggatcgtcg cccgccacgt cgatccggac tatcgcaaac gcatggaggt cgaagccgtt
     3481 ctgacggcac tgagggctgc acgaggctga acgcggtcac gcacgggggc tgtcagccca
     3541 ggcgcgtgcg cggagcagct tcatgagggc atcgcctccc gccgaacgct ggcgtcccgc
     3601 cacgccgtag agcgacacct tgcgtgcaag atccagatcg gcaaccgcaa ttcgccgaag
     3661 gtgcgcgtag tggccggcgc tctgcggcag gagtgcgaca cccaggttgg ccgcgaccag
     3721 ggccaccaca tcgtcgagcg agttcaattt gtggttggta acactggcaa tccggcgctc
     3781 gttcaagatc tgcgccaggc tgtcggcctg ttcgcactgc tcgctgatca gcaagcgctg
     3841 ctcggcgagc ttgcccgcat ccacggcttg ggcgttggcg agcggatggt cgcgatggat
     3901 agcgagatcg aacctttcgt cgaatagcgg ccaggcctcg agccggtccc aggtctgctc
     3961 gagcggaccg gcgatggcga attccacccg ccccttctgc agggcttcgg cgatctcacc
     4021 cgcgttgccg cgcagcacct tgagctgcag gcctgcaacg gcgcgctgca gctctgtgag
     4081 caccggcgtg agcagccgca tatcgatcgt gtgcgacagc cccaaggcca aagggctggc
     4141 tacccccttc ttcagcgatg atgcgagcga tttcgccgcc agagcactgt cgtagcactg
     4201 ctgcatgagg gggagcatgc ggtgcccgag ctcggtcaag tgcgagagct tgccctcgcg
     4261 ccgcaggagc tccccaccca actcctcctc gagctggcgg atggcgcgtg tcagggccgg
     4321 ctgggagacg ttgcactgct cggccgcgcg cgtgaagttg agggtgcgcg caaccgtgag
     4381 gaagtagcgg acctgatgca tttccatcgt tgaggcgtcc cgacgctcgt tcaagaccgt
     4441 cgcgccgccg tgcgtcgcgc tcagtcctgc aaccagaata ggtgcccggc tacgtggttg
     4501 ggaagccgtc gcgtggaggg agcctcaacg gcagatgcgc gaccccaccc cggcgcccag
     4561 cgccatgctc gcgtcccgcc cgccatggcc atgcgcgagc ggcatcgaat cccaagccaa
     4621 tcggtatttc agatcgtgct ctgcgctcgg cattgtccct cgtatggcgg cgaaggagcc
     4681 gcccgccatc gagaggacag gagaccacca atgctgaacg ctacatccac gagccggtcg
     4741 agctggcacc gacttgcggc ggccggttcg ctcaccgtgg ccgtgctgct cgcccccgcc
     4801 gcggcctccg ccggcgcatg tccggccggc aagtccgtgg ccgacggcca gggacagaag
     4861 atgagcaccg ccaaggcgaa gggcgtcacc gacaaggtgc aggcgaggat cgatctcgcg
     4921 aacgagcccg tcgcgctgaa ggagcacgcg ctgcgcctgc gccggctcgt cattcagccg
     4981 gggggaatcg tcccctggca cagccacacc gaccggccgg cgatcatcta catcatcagt
     5041 ggtacgatca ccgagtatcg cagcacttgt gcggtgccga tcgtgcatcg ggccggcgag
     5101 tctaccgccg aggtgaaagg cacctcgcac tggtggaaga acaccggcaa gacgaccgtg
     5161 gtgctgctgt cggcggatct cttccacgac aaggacgacg cgcacatgat gtgaccgcgg
     5221 cctcggcgcg gtccggacta gacgccatac cggagtgcgg cagatccctc ctcccgccgt
     5281 tccccctgac ggtgcgccgc actccggccc ctccagccat gagaaaccac catgccacag
     5341 gccaccgaag aatacgcagc gcttccgccg gcccgcgagc tgacgggcaa gaccgcgatc
     5401 gtcaccggct cgaccagcgg catcggtctc ggcatcgtgc gcgcctttgc cgaagccggc
     5461 atgcacgtga tgctcaatgg cttcggcgac aaggccgaaa tcgaacgaac gcggatggag
     5521 ctcgaggagc gcttccatat caagaccgcc tactccgcgg ccgacatgtc caagcccaca
     5581 gacatcgtcg gcatggtcga ggacgcgcgc cggctcctgg gcaaggtcga cgtgctcgtc
     5641 aacaatgccg gcatccagca tgtcgaagcg atcgaaacat ttcccatccc caagtgggat
     5701 gcgatcatcg ccatcaacct ttcgtcggcc ttccatgcca cgcgtgcggt ggtgccggag
     5761 atgaaagcgc gcaaatgggg gcgcatcatc aacatcgctt cggcgcacgg actggtcgcc
     5821 tctcccttca aatcggccta tgtggcggca aagcacggcc tgctcggcct caccaagacc
     5881 gtggcgctcg agacggccga gcacggcatc accgccaacg ccatctgccc cggctacgtg
     5941 ctgacgccgc tcgttgagaa gcagatcccc gagacggcca aggcacgagg cctcaccgag
     6001 gagcaggtgg tgaaggacgt gctgctccac gcccagccga cgcgccagtt cgtgacgacg
     6061 ggccaagtcg gcgcacttgc gctgttcctg tgcaccgatg ccgctgcatc catgacgg
//
LOCUS       DASRHN010000157        21477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_53256, whole genome shotgun sequence.
ACCESSION   DASRHN010000157 DASRHN010000000
VERSION     DASRHN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21477
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53256"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFB37_05995"
     CDS             complement(<1..1146)
                     /locus_tag="VFB37_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZO22040.1"
                     /translation="MSCARAALMALALIAAGCSRQPAVVQAPPPPEPKVTAQAAWTEF
                     ASRFIEDYFQSHPFFAVQAGRHEFDGQMGDWSATGLQKEIDRLKHVRAQAEAISPGPL
                     EAAQRFEREYLLSVIDRDLFWLDQARFPFSNPAWYIERLDPEVYLSRNYAPLETRLKG
                     YMGYAQAIPHIATEIRANLHTPMPRPYIERGIAGFGGFAQFYRNDAPKVFASIQDPQA
                     QRDLRAASTAAAKAMDELKSWLVSQRASGTDSFALGPELYAAMLKQTERVDVPLADLV
                     AIGRADLERNQQALKQACAQFLPQGSLRDCIARMNAHKPAGGTLEGARAILKELKAFI
                     VAKQIVSIPGAEEALVAEAPPYNRGNFAYINIPGPYEKGVASTYNISP"
     gene            complement(1178..2707)
                     /locus_tag="VFB37_06000"
     CDS             complement(1178..2707)
                     /locus_tag="VFB37_06000"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HZO22041.1"
                     /translation="MSESVAAVRGQTDDKRIDVSLLSHTNVGKTTLARTLLRQDIGEV
                     GDRAHVTEVAESHVLIESPQGDSLVLWDTPGFGDSTRVLKRLQQSDRPVGWFLSQVWD
                     RLADRPFWCSQQAMRTARETSDVILYVVNAGEQPVSAGYVDAEMRILGWIGKPVVLLL
                     NQLGPPRESVHAQADVKLWEGSAAKYPWVEQVLPFDAFARCWVQEGTLLAHLQKLLPT
                     ARQASFGRLRERWRERNLEVFHHSMQALAGQIAAAVVDAEPVEELDVQGKARAWLGAV
                     TGVAVREDARLAAAQGALAARLDRQVRATTEELVRLHGLTGKATEEVLKRMGREFAVD
                     RPADVDKASVLGGLLSGALGGLAADLGAGGLTFGAGALIGGVLGAFGARGLAKAYNLA
                     RGAHAATVRWSGDFLFERVAAALARYLAVAHFGRGRGEFVSSSAPPHWQEAVVSALRQ
                     RRSDLDAVWERRNEGSGATGAGAEPPGARAVSAALLPIIAASAQQILLRLYPDSSDVF
                     R"
     gene            complement(2704..4242)
                     /locus_tag="VFB37_06005"
     CDS             complement(2704..4242)
                     /locus_tag="VFB37_06005"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HZO22042.1"
                     /translation="MHQQALRTVLLIQAIEETDRLGEVIPLADRADASRAALRELGST
                     GLEPSAGALSPASQGFIVHRAERLLASVRSRAPAVGHALALAGGFTWLGRVVLVVAFA
                     FGVSLAALDGSRRINILAFPLIGLIAWNLVVYVLLFTAWLGALRRSEAVSARPVTRTY
                     SRWIAARIESLLRRSTRFNVPLTNGLRRFAGEWTVISQPLWIFTGKRLMHLAAALLAL
                     GLIAGLYVRGIALRYEAGWESTFLGPRSAQALITILYGPASALSRIPLPSLDEIRALR
                     WTASGGGGDAARWIHLIALTAVLYIVIPRLLAVLTATFGLWRFSRRPPLPASLNGYAR
                     SVLLSVGDGTVREVASVTPYAYEPAEESIAGLELFLADALGGKLTVSVREPIRYGEEE
                     KAASLVPANWNVLLLSLAATPEVENHGMLISGWRDWLAHNASAAPLLVMLDEAPYAAR
                     MRNDMGFEDRLRQRRQLWSEFVSGYGLRACIVDLTRITPGSQEGQNARDAARRALWTA
                     SESA"
     gene            complement(4332..4652)
                     /locus_tag="VFB37_06010"
     CDS             complement(4332..4652)
                     /locus_tag="VFB37_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HZO22043.1"
                     /translation="MKRHKTREIDALARRIASLDERSIDSLYGLEPVYEPGKGLPGLQ
                     PEEFVAVQCPYCGERLETRVDLTASDESSYIEDCSVCCRPIEFGIERNENGALRAVKA
                     RRLD"
     gene            4880..5209
                     /locus_tag="VFB37_06015"
     CDS             4880..5209
                     /locus_tag="VFB37_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HZO22044.1"
                     /translation="MTTVNVGFAILAFGVLASGNAIAAPQSQTETVEVRYTRAALDAP
                     GGAERLYRRIQAAARQACGDSDPRDIVRYFMFKGCYQNALDAAVEKVNASTLTALHHK
                     AQRTAPG"
     gene            complement(5279..5980)
                     /locus_tag="VFB37_06020"
     CDS             complement(5279..5980)
                     /locus_tag="VFB37_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22045.1"
                     /translation="MRIAGCLCALTSLSLVGCFGVSTLDPAAPHSIDLSGNWILDRQA
                     SDDPQKLLDKLRPKETTHRDMSAADDGLGPYDNGQQDGQGGNGPRGRRSQQAMQPLLY
                     RNNQIITHTQVMRALSADVARADQLTIRQTPTQLTLDYGITARTFTPGLKSVVSASWG
                     VADQSSGWKGKEFVIEVKPQTGVRSVESYTLSSDGKHLIEQLHLGGGDYPNVALKRVY
                     DHTDQPLPRSIPMND"
     gene            complement(6019..7413)
                     /locus_tag="VFB37_06025"
     CDS             complement(6019..7413)
                     /locus_tag="VFB37_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZO22046.1"
                     /translation="MPAQRIDAAGGEHVDQRAVLALALPLMANSAIQIILNLTDMWFI
                     GHISTKALAGVGAVQWLVLVVVLILGGVASAVQTITAQSYGARRYTRASQAVWTALWA
                     TLCAAPLFVAAGAARHAILAPFGFDPTIELLASQFWFPRVGGAAFGVAVWAFLGFFNG
                     IGRPRISLIVTVVMAVANAVFNNFFIFHLGWGVAGSGWASTAAQAIGLSLCALIFLNA
                     SYRARFRSHLTWRPHLHQLGEQLRLGLPTGLLPAADLLGISFFQMMQVRLGTAGGAAT
                     QVAMLLTSVAYMPGYGIASAGTTLVGQSIGAGDRQWGMRVGTRVILLTALYMGGIGVV
                     LALAGPLLLPVFFGAHDAEAVAAVGLGEQLLWLAAIYQFFDGLNMGSALCLRGAADVI
                     VPAVLVIPLSWLVFVPLAHAFTFAPGRGWVHFLPQFGWGPIGGWSALVIYVMLLGTIL
                     FLRWRSGAWLRIRL"
     gene            7565..8584
                     /locus_tag="VFB37_06030"
     CDS             7565..8584
                     /locus_tag="VFB37_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZO22047.1"
                     /translation="MATRKAKNPAATRGKGAGAKRRRGKLAPGKSARGLDAAEVAIAI
                     DSPEVAAVATLVAQAGGAPIGAYHDPLGGRPLLLASLPLTAVQPTPFQRDLSPTHAKR
                     LAEKIDETAAFLDPLIVVRGEDGRLWTPNGRHRLAAAKVLGLKQITALISPDESLAYR
                     ILALNTEKAHNLRDRSLEVIRMARNLAKTQGSAHESQFGAEFESAELLTLGIVYEKSP
                     RFAGGAYGAFLKKVDRFSERTLAASLRERESFAARLLEIDAQAKRVMAALQTRGFKSP
                     YLRNYVVARINPVRFHKVKKGDTKPPMPLGQALIRMAAAAKAFKIESVSNRDLAWVAV
                     GAGSE"
     gene            complement(8612..10645)
                     /locus_tag="VFB37_06035"
     CDS             complement(8612..10645)
                     /locus_tag="VFB37_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HZO22048.1"
                     /translation="MRGPAGKGAGEVSTRRLGRQRAPGGALLCSSVVLVLGTGLGTSA
                     AGGRLAAPPEGLVHPAEWPQVPPAIARDADLEARISRLLAAMRPEQQVGQLLQADIAH
                     ITPDDLRQFPLGSVLNGGNSSPGNNVFAPPREWLALADRFYEASVDPVQGPRPIPTLW
                     GIDAVHGVAKIPGATIFPHNIGLGATRDPALLREIGAVTALEVRTIGLDWTFAPTLAV
                     ARDVRWGRTYESYSENPQLVRELARQMVLGLQGEPGTAQFLDSTHILATAKHFVGGGG
                     TDGHDEGDNLSSEAQLRDIDFAGYPGAVAAGVQTVMTSYSSWHGTKMAAQRSLITDVL
                     KGRLHFDGLVIGDWNAQGQIPGCSNVSCAKAINAGLDMFMAPDSWKGLFDNTLRQVRT
                     SEIPPARFEDAVRRVLRVKLRAHLLEEGKPSLRPMAGRFDLLGSSAHRALARRAVRES
                     LVLLKNRDQVLPLSPRQRVLVAGDGADNIAKQSGGWTLRWQGTGLANQDFPHSESIYE
                     GIRAAVVAGGGTAQLSSTGSFTVRPDVAIVVFGENPYAEAAGDRPTVQFSPGDKSDLQ
                     LLRSLQARRIPVVSVFLSGRPLWVSPELDASTAFVAAWLPGSEGGGVADVLFRAPDGS
                     VRFDFRGKLPFSWPRTPHQSGTADGEGSERPLFPYGYGLSYSSSLDVAPRKPQ"
     gene            10791..11528
                     /locus_tag="VFB37_06040"
     CDS             10791..11528
                     /locus_tag="VFB37_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO22049.1"
                     /translation="MLGDLPDLLLVERTRGRDTRAFETLMRRYNRRLYRIARSILTRN
                     DQAEEAVRGAYLRAFANLDRYEPAGKFGAWLARLAFNEALALRRHLATAGSPADKVER
                     PTPTVGSNETALEPGGPGAAEGHTVNSTSSSAALVSSEMASTEMSEQMLELAIDTLPE
                     VFRTVLVLRLIEELSGVEVAVCLGLNETTVRTRLFRAQQRLKVDIARRLREGPANIFE
                     LTPERADRIVSQVFARLHTAIPTSEPR"
     gene            complement(11587..12189)
                     /locus_tag="VFB37_06045"
     CDS             complement(11587..12189)
                     /locus_tag="VFB37_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZO22050.1"
                     /translation="MPTLSGLKVAILVADGFEQVEMTEPRKALNEAGAETQIVSPAKG
                     QVQGWNHFDHADRFEVDVPLQEAEAQDFDALVLPGGVANPDQLRTMPQAVKFVRSFFE
                     AKKPVAVICHGPWTLVEAGVVKGRSITSWPSVKTDLVNAGARWTDSEVVVDQGLVSSR
                     KPADLPAFNRKMLEEFASASRARASESRSASEGAQPAARS"
     gene            12572..12829
                     /locus_tag="VFB37_06050"
     CDS             12572..12829
                     /locus_tag="VFB37_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22051.1"
                     /translation="MPDTMWFDEDELRIEARRRVKSGRLPCQPPQYMWAGAGDGQRCS
                     LCDRAIDAQQIEYELEFSTQPSVIHRFHRICHEAWELECIR"
     gene            12973..14622
                     /locus_tag="VFB37_06055"
     CDS             12973..14622
                     /locus_tag="VFB37_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22052.1"
                     /translation="MKLLCSLPGLLAGLTLTLALGSVQAKEGDHEAGRPDHAASEEFP
                     SPRYLEDQSQSTSAAINARGAKIAYQAEAGVQVVYLKDPKDEDPPPPHDEHNNPPPPP
                     VHATMSYVAYFKGDKPDPHRPITFLFNGGPGSSTVWLHMGAFGPRRVVTANDSHSPAA
                     PYRIVDNEYSLLDASDLVFVDAPGTGFSHLRGPDKEKSFYGVDEDGHAFANFIVGFLS
                     KHGRWNSPKYVFGESYGTTRAALLAYILQGEKAVDLNGVILLSQILNFDDNADGPQFN
                     PGVDQPYVLALPSYAASAWYHHKLPEQPAALEPLLAQVEQFALTDYWPALMAGNHLSP
                     QRRSEIAAKLHEYTGLPTDYIERADLRINVGEFEKNLLGSEITTGRLDSRFSGPTIDP
                     LSKEADYDPQSASISSAYVSAFNEYVRTTLKFGTNMIYKEVIDVEKTWDYQHQPPGSP
                     VKLPQAPNVELALAFAMKTNPNLKVMMNGGYYDLATPFFAAVYELEHLPIQPELTKNI
                     EMHFYTSGHMVYAHEPDLKALHDNVADFIHRTEASGGAGTQ"
     gene            complement(14637..15380)
                     /locus_tag="VFB37_06060"
     CDS             complement(14637..15380)
                     /locus_tag="VFB37_06060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO22053.1"
                     /translation="MNPRIALVTGAGSGIGRGVALALAKAGYVVVLTGRTLASLQATA
                     AEAGSQASVIAADVADRQSVAALFAQVASQFGRLDVLFNNAGTGAPAAPLEEVTLEQW
                     QSVIDTNLTGTFLCTQAAFRLMKTQQPRGGRIINNGSVSATTPRPHIIAYTASKHAVT
                     GLTKATALEGRAYDIACGQIDIGNAATDLAAHMTHGTLQADLSLRPEPSIDVKHVADA
                     VLYMAGLPLQANVLFMTVMATRMPFVGRG"
     gene            complement(15377..16168)
                     /locus_tag="VFB37_06065"
     CDS             complement(15377..16168)
                     /locus_tag="VFB37_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZO22054.1"
                     /translation="MLRFAANLGLLWPDRPLLARLEAAAQAGFRAVEMHWPYEVAAET
                     LGEVARRNQLTLLGINTSPGQFEAGERGLGALIGRELHFQASVDQAIAYCRIAGARAI
                     HAMAGNVPSAERLRARAVFRDNLRVAAAKAASHGLTLLLEPLNSCDNPGYFYSKLEEA
                     VSLLEELNLPNLKLQFDVYHVARSEGDVLRKLRQLTSWLGNVQIAAVPSRAEPDEGEI
                     AYPAIFQALEESGYTGWVGCEYRPRGDTGAGLAWLTHLGVTGGLR"
     gene            16279..16758
                     /locus_tag="VFB37_06070"
     CDS             16279..16758
                     /locus_tag="VFB37_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZO22055.1"
                     /translation="MSGFYDIEVKAIDGTPDLLGRLRGKVSLAVNVASRCGLTPQYAG
                     LEELQRDLQDQNFTVIGFPCNQFGAQEPGTEQQIVQFCQSRYDVSFPMSAKLEVNGSG
                     RHPLYQFLTSAGTGVAGDITWNFEKFLIGRDGKVLKRYPPPTKPQDSGLMQDIADAL"
     gene            16797..17117
                     /locus_tag="VFB37_06075"
     CDS             16797..17117
                     /locus_tag="VFB37_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HZO22056.1"
                     /translation="MASDSCLSRRSLLGKGLLAALLPLVLARSDPARAKDAALLSPSD
                     PAAQKLKYTEEAAKTKGVPPGNSCANCALYQGSYSSKQGPCQLFPGKQVRASGWCSSW
                     TAQM"
     gene            complement(17097..18590)
                     /locus_tag="VFB37_06080"
     CDS             complement(17097..18590)
                     /locus_tag="VFB37_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZO22057.1"
                     /translation="MLELSITLPPRGSGSVMSALHRELRAAILDGRLKPGLRLPSTRL
                     LAHSWGVSRNTVMGAYDLLLSEGYLCARHGAGTYVAISLPVHTRAATAHLPAADPRLN
                     AFWRAPPADLGTSWASAAPGNAPFRIGVPDAEAFPFHIWRRLLARSLRALAKSPAAFV
                     EAQGRTALREAIAHHASFARAVACEPDDVVVTSGTQQAFELLAKILVTPGETSVAFED
                     PGYPPLRAAFIANGARIIPAAVDSEGLVVDSLPAETRLICVTPSHQFPLGVAMSMRRR
                     AQLLEFARRSGAVVIEDDYDGEFRFGSTPRDALQTLDRSGSVFYVGTFSKSLFPGIRL
                     GFVVVPRWARHALIAAKQCADWHSPVLEQDALAAFIGEGHMARHVRKMRKLYGERRTL
                     LVSVLQGQFAPWLEPIPASGGMHLTALERQALDWDNIAAQARELGLDVRSLRVYCVGS
                     RSRRGLVLGYGGTSPSAIRSGLSQLRRLFPMDPMRPAARAVSHLCGP"
     gene            complement(18583..20061)
                     /locus_tag="VFB37_06085"
     CDS             complement(18583..20061)
                     /locus_tag="VFB37_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HZO22058.1"
                     /translation="MPDTLSLDRRALDRARLSRDPRFDGKFFTAVTSTGIYCRPVCPS
                     PPSKSTNVRYYATAAAAAEAGYRPCLRCRPEAAPGTPAWMGTSAVVRRALRLIDSGAL
                     DSDSVSALAARVGVGTRHLGRLFSHHVGASPITVAQTRRLHFAKRLLDETDLPVTQIA
                     LASGFSSIRRFNSAFQATYERPPRQLRRRAGEPARCEPGDEVALRLAYRPPYDWMQVR
                     KFLAARALPGVEHVDEHGYARTVGLSSGPAVVRVRPLPEADALEFRVRGAPAAALLQL
                     SGIARRTFDLAADPTRIAQVLRSDALLAPLVRNQPGLRIPGAWDPFECAVRAILGQQV
                     SVATGRTFVSRLVARAGSRIDCGTDRLTHLFPTPEQVAAAELEGLGVTAPRVGALRAM
                     TRALIEGRLDFNAPPEEVAAVLASLPGLGDWTAQYVVLRALGEPDAFLAGDLIVRRAA
                     GNGGTLLSARELSKRAECWRPWRGYAVLHLWTNDCRHRVSHA"
     gene            20197..20847
                     /locus_tag="VFB37_06090"
     CDS             20197..20847
                     /locus_tag="VFB37_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZO22059.1"
                     /translation="MSAIISAFLLTFAGLFPIVNPIEAAPFFLALTPNLAPAERNALA
                     RRIAVNGFALLLGSMVLGPWLLEFFGIELPVVRIAGGFVVTALGWKLLTQEDWSDHGS
                     APLDPTSGRRIGTFYPLTMPLTVGPGSISVAITLGSKKPATGFRLADVALHAVGALTG
                     VIAIAASIYIAYRFSGNIARLLGATGLEVVVRLSAFILMCIGIEIVWNGYSTLIAH"
     gene            complement(20834..21283)
                     /locus_tag="VFB37_06095"
     CDS             complement(20834..21283)
                     /locus_tag="VFB37_06095"
                     /inference="COORDINATES: protein motif:HMM:NF018425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZO22060.1"
                     /translation="MSRTYPSKVDWPILLPALALPVIFVVTARVVSVMQPHALSAAVR
                     IVFFLVVLFAMWTLLGTSYTLDATMLQVRCGPFRWRIALDEIHSVTATRDSRSGPALS
                     LDRLRIDYGSGRSMLISPRDQQAFLRDLEHRRRGPGAGAPRAAISAQ"
     gene            complement(21298..>21477)
                     /locus_tag="VFB37_06100"
     CDS             complement(21298..>21477)
                     /locus_tag="VFB37_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22061.1"
                     /translation="IEGLKVMTRNGWFAARPSGTEDIYKVYGESFLGKEHLERILEEA
                     QRIVSDCFAAESPKA"
BASE COUNT         3956 a         7186 c         6915 g         3420 t
ORIGIN      
        1 gggtgagatg ttgtaagtgg aagccacgcc cttctcgtaa gggccgggaa tgttgatgta
       61 ggcgaagttg ccccggttgt aggggggcgc ctcggcaacg agcgcttcct ccgcccctgg
      121 aatgctcacg atctgtttgg ccacgatgaa ggccttgagc tccttcagga tggcccgggc
      181 gccttcgagt gtccccccgg cgggcttgtg ggcgttcatc cgcgcgatgc agtcgcgaag
      241 cgacccctgt ggcagaaatt gcgcacacgc ctgcttcagg gcctgctgat tgcgctcgag
      301 gtctgcccgc ccgatcgcca cgaggtccgc cagcggcaca tcgacccgct cggtctgttt
      361 cagcatcgcg gcgtacagct ccggacccag ggcgaagctg tccgtcccac ttgcgcgctg
      421 tgagacaagc caggacttga gttcatccat ggctttggcg gccgcagtgc tcgccgcccg
      481 caggtcccgc tgcgcctgcg ggtcctggat cgaggcgaag accttgggtg cgtcgttccg
      541 atagaactgt gcaaaaccgc cgaagccggc aattccccgc tcgatgtagg gccggggcat
      601 gggggtgtgc agattcgcgc ggatctcggt cgcgatatga ggaatggcct gcgcataccc
      661 catgtagccc ttcagtcggg tctcgagtgg cgcgtagttg cggctcagat agacctcggg
      721 gtcgaggcgc tcgatgtacc aggctggatt gctgaacggg aagcgcgcct gatccagcca
      781 gaacagatcc cggtcgatca cgctgaggag gtattcccgt tcgaagcgct gcgcagcctc
      841 cagaggaccc ggactgatcg cttcggcctg ggcgcgcacg tgcttcagcc ggtcgatctc
      901 cttttgaagc ccggtcgcac tccagtcgcc catctgacca tcgaactcgt gacggcccgc
      961 ctgcacggca aagaacggat gcgactggaa gtagtcctcg atgaagcggg acgcgaactc
     1021 ggtccacgcc gcctgcgcgg tcaccttcgg ctcgggtggg ggcggtgcct gaaccacggc
     1081 gggctggcgg ctgcaacccg ccgcaatgag ggccagagcc atcagggcgg cacgcgcgca
     1141 ggacatcgaa aacctcatga atacctccga ttcaccatca tctgaatacg tccgacgagt
     1201 cgggatagag ccggagcagg atctgctgag cactggccgc gatgatcggc aacagcgctg
     1261 ccgatacagc cctcgcgcct ggcggttccg cacccgcccc cgtggcgccc gacccttcgt
     1321 ttcgcctttc ccacaccgca tcgaggtctg accgtcgctg ccgaagcgca ctgaccactg
     1381 cctcctgcca atggggcggt gcggagctgg agacgaactc gccccgcccc cgtccaaagt
     1441 gagccaccgc cagataccgc gcgagcgccg cggccacgcg ctcgaacagg aaatcgccag
     1501 accatcgcac cgttgcggca tgcgcaccgc gcgcgaggtt gtaggccttg gcaagccctc
     1561 tagcaccgaa cgcgcccagc acgcctccga tgagggcgcc cgcaccaaag gtgagtcccc
     1621 cagctccgag gtcggcggcg agcccgccca gggcgcccga gagcaagccg cccagcacgc
     1681 ttgccttgtc cacatcggcg ggtcggtcga ccgcaaattc gcgtcccatg cgcttcagca
     1741 cctcttcggt cgccttcccc gtcaggccat gcagtctgac gagctcctcg gtggtggcgc
     1801 gaacctgccg atccaggcgc gcggcgagcg cgccctgcgc cgcagcgagc cgagcatcct
     1861 cgcgcaccgc gactccggtg accgcgccca gccaggcgcg tgccttcccc tgcacgtcga
     1921 gctcctccac aggctctgcg tccaccaccg ctgccgcaat ctgcccagcc agcgcctgca
     1981 tcgaatggtg gaagacctcc agattccttt cgcgccagcg ctcgcgcagg cgtccgaagc
     2041 ttgcctggcg ggccgtcggt aacagtttct ggaggtgcgc cagcaaggtc ccttcctgta
     2101 cccagcaacg ggcgaacgca tcgaaaggca gaacctgctc cacccaggga tatttcgcgg
     2161 cactgccttc ccagagcttc acatcggcct gagcgtggac cgactcgcgg ggtggaccga
     2221 gctgattcag cagcagcacc accggcttgc cgatccatcc gaggatgcgc atttctgcat
     2281 cgacgtagcc ggcggaaaca ggctgctcgc ctgcattgac cacgtacagg atgacatcgg
     2341 aggtctcccg cgcggtgcgc atggcctgct ggctgcacca gaagggccgg tctgccaacc
     2401 gatcccagac ctgggacaga aaccagccga cgggtcggtc gctttgctgc agccgcttca
     2461 gcacccgtgt gctgtcaccg aagcccggtg tgtcccacag gacgagcgag tcgccctgcg
     2521 gtgattcgat cagcacgtgg ctttcggcga cttcggtgac atgcgcccga tcgcccactt
     2581 cgccgatatc ctgccgcagc agggtgcggg ccagagtcgt cttgccgaca ttggtgtgcg
     2641 acagcagact gacatcgatc cgtttgtcgt cggtctgtcc tcgcactgcg gcgacactct
     2701 cgctcaagcg ctttcgctcg ccgtccacag agcacggcgg gccgcatcgc gtgcattctg
     2761 gccttcttga gagcctggcg tgatccgggt caggtccacg atgcaggcac gcagcccata
     2821 accggataca aactcgctcc agagttggcg gcgctgccgc aggcggtcct caaatcccat
     2881 gtcgttccgc atccgcgcgg catagggagc ctcatcgagc atgacgagca agggcgcagc
     2941 gctcgcgttg tgcgccagcc agtcccgcca gcccgaaatc aacatgccgt gattctcgac
     3001 ttcgggtgtc gcggccagcg acagcagcag cacgttccag ttggccggga ccagggaggc
     3061 ggctttttcc tcctcgccgt aacgaatggg ctcgcgtacg ctcacggtca gcttgccgcc
     3121 gagcgcatcc gcaagaaaca actccagacc tgcgatcgac tcctccgcag gctcgtaagc
     3181 gtagggcgta acactcgcaa cctcccgaac ggtgccgtcc ccaacactca gcagcacgct
     3241 gcgcgcatac ccattcaggg aagcgggaag cggcggccgt cgcgagaagc gccacaggcc
     3301 gaaagtcgcc gtgagcacgg caagcagccg cggaatcacg atgtacaaca ccgcggtcag
     3361 ggctatcaga tggatccagc gtgccgcatc gccgccgccg ccgctggccg tccagcgcag
     3421 agcacgaatt tcatcgagcg atgggagcgg aatgcgcgac agcgcggaag ccggtccata
     3481 caggatggtg atcagtgcct gagcactgcg cgggccgaga aaggtgcttt cccaccctgc
     3541 ttcgtagcgc agcgcgattc cgcgcacgta caggccagca atgagcccca gcgccagcag
     3601 tgccgcggcg agatgcatca gccgcttgcc ggtgaaaatc cacagcggtt gcgaaatgac
     3661 cgtccattcg ccggcaaatc ggcgcaggcc gttggtcagg ggaacgttga agcgcgtcga
     3721 gcggcgcaga agcgactcga tgcgagcggc aatccagcgg gaataggtcc gtgtgacagg
     3781 gcgtgcactg acagcctcgg aacggcgtag cgccccgagc cacgccgtga acagcaggac
     3841 gtatacgacc aggttccagg caatgagccc gatgagcggg aaggcgagga tgttgatgcg
     3901 ccggctgcca tcgagcgccg ccagggagac gccgaatgca aacgccacca cgagcacaac
     3961 ccggccgagc caggtgaatc cgcccgccag cgcaagcgca tgccccacgg ccggagcgcg
     4021 cgagcggacg ctggcgagga gtcgttcggc ccggtgcacg atgaagccct gcgatgcagg
     4081 agagagtgca ccagcggagg gctccagccc cgtggatccg agctcgcgca gggcggcgcg
     4141 cgaggcatcc gcccgatcgg cgagcgggat gacttcaccg agtcgatcgg tctcttcgat
     4201 cgcctggatc agcagaaccg tccgcagcgc ctgttggtgc atggctggct ggcgggctca
     4261 acggtccgct ctgcaggccg aaagcggctg cagaggggag ccgccgggcg cgcgggacga
     4321 ctgatggctc actagtccag tcgccgtgct ttgaccgccc gcagcgctcc gttctcgttg
     4381 cgctcgatgc cgaattcgat cggccggcaa caaacgctgc agtcctcgat gtacgacgac
     4441 tcgtcgctcg cagtcagatc gacgcgagtt tccaaccgtt caccgcagta cggacactgt
     4501 acagccacga actcctcggg ttgcagccct ggcaggccct tgccgggctc gtacaccggc
     4561 tcgagaccgt acaggctgtc gatgctgcgc tcatcgaggc tcgcgatgcg gcgcgccagc
     4621 gcatcgatct ctcgggtttt atgtcgtttc acggcggcca ttctacgcga ggggcgccca
     4681 caaccccgcg gaaattgcgg ccgcgacctg cagccaggtg gccggaagcc gccacggttc
     4741 gtcgccattc gccacggctg agccgaagcg acctgctgtc acgaccggcg gagcaaaccc
     4801 aaatgccccg gcgtgcatct acacggatgc tcgaagtgaa aacgagcgca gtcatccgcc
     4861 ctccaacagg agaaccatca tgaccaccgt gaatgttggc tttgccatcc tggccttcgg
     4921 tgtgctcgcc agcggtaacg caattgcagc tccgcagtct cagaccgaaa ccgtcgaggt
     4981 gcgctacacg cgcgcggcgc tcgatgcccc gggcggcgcg gaacgcctgt atcgacggat
     5041 ccaggcagca gcccggcagg catgcggtga ctccgacccg cgcgacatcg tgcgctactt
     5101 catgttcaaa ggctgttatc agaacgcgct cgatgcggca gttgaaaagg tcaatgcctc
     5161 cacgctgacg gcacttcacc acaaggctca gcgtaccgct cccggttgag tcagtcagga
     5221 gggcgcgtga gcaggcgggc atccgccgtc agctcacgcg cacgaggcct ggcgcgactc
     5281 agtcgttcat gggaatcgag cggggcagcg gctgatccgt gtgatcgtat acgcgcttca
     5341 gcgcgacatt cgggtagtcg ccgccgccga gatgcagctg ctcgatcagg tgtttgccat
     5401 cgctcgaaag cgtataggac tcgacggagc gcacgccggt ctgcggcttc acctcgatca
     5461 cgaattcctt gcccttccac cccgagctct gatcggcgac accccaggag gccgacacga
     5521 cgctcttcag gccgggtgta aaggtgcgcg cggtaatgcc gtaatcgagt gtcagttgag
     5581 tgggggtttg acgaatggtg agctggtcgg ctcgggccac atccgcgctc agcgcacgca
     5641 tcacctgggt gtgcgtgatg atctggttgt tgcgatagag cagcggctgc atggcttgct
     5701 gcgaccggcg cccgcggggt ccgttgccgc cctgaccgtc ctgctgaccg ttgtcatacg
     5761 gccccaggcc gtcatcggct gccgacatgt cacggtgagt cgtctccttg ggccgcagct
     5821 tgtccaggag cttctgcgga tcatcacttg cctgacgatc gaggatccag ttgcccgaga
     5881 ggtcgatcga atggggcgcg gctgggtcaa gggtgctgac gccgaagcat cccaccaagc
     5941 tcagcgaggt cagcgcacac aggcagccgg cgatgcgcat gggggcagtc actcctgcaa
     6001 ctcgcgagcg gttcgagctc acagcctgat ccgcagccag gcacccgagc gccagcgcag
     6061 gaacaggatc gtgcccagca gcatgacgta gatcaccaag gcgctccagc ctccgatcgg
     6121 cccccagcca aactgaggta gaaaatgcac ccatccccgc cccggagcaa atgtgaaggc
     6181 atgggcgagg ggcacgaaca ccagccagga gagagggatg accaggacgg ccggcacgat
     6241 cacatctgcc gcccctctca ggcacagggc actgcccata ttgagcccgt cgaagaactg
     6301 atagatcgcg gcgagccaca ggagttgctc gccgagcccg acggcggcca cggcttcggc
     6361 atcatgggca ccgaagaaca ctggcaggag cagcggaccg gcgagagcca gaaccacccc
     6421 gataccgccc atgtacaggg cagtcagcag gatgacccgg gtgccgaccc gcatgcccca
     6481 ctgccgatcc cctgcgccga tcgactggcc gacgagggtc gtaccagccg aagcaatgcc
     6541 gtagccgggc atgtaggcga cagaggtcag cagcatggcc acctgggtgg ccgcgccgcc
     6601 ggcggtgccg aggcgcacct gcatcatctg aaagaaggag atgcccagca ggtcggcggc
     6661 cggcagcaac ccggttggaa gtccgaggcg cagctgctca cccagctgat gcagatgcgg
     6721 ccgccaggtc agatgcgatc tgaagcgcgc gcgatacgaa gcattcagga agatcagcgc
     6781 gcacagtgac aagccgatgg cttgtgcagc ggtcgacgcc cagcccgaac cggctacgcc
     6841 ccaaccgagg tggaagatga agaagttgtt gaacacggcg ttggccacgg ccatgacgac
     6901 cgtgacgatg agcgagattc ggggcctgcc gatgccgttg aagaagccga ggaatgccca
     6961 gactgctacg ccgaaggcgg caccgccaac gcgcggaaac cagaactgcg aggccagcag
     7021 ctcgatcgtg ggatcgaagc cgaagggagc gaggatcgcg tgccgtgctg ccccggcggc
     7081 gacgaacagc ggagcggcgc acaaggtggc ccagagcgcg gtccacaccg cctgcgaggc
     7141 tcgcgtgtag cggcgcgcgc cataggattg ggcggtgatc gtctgcacag ccgatgccac
     7201 gccaccgaga atcaacacca ccaccagcac cagccactgc acggcgccga caccggccaa
     7261 tgccttcgtg gagatatgac cgatgaacca catatcggtg aggttcagaa tgatctgaat
     7321 tgcgctgttg gccatcaggg gcagcgcgag cgcgagcacc gcgcgctgat ccacgtgctc
     7381 gcctccagca gcgtcgatgc gttgcgcggg cattgttccg gcaacgcggg ttggctcgat
     7441 gcgcgtatcc atgggcgata gggggaccgg cagtgaaggc tgcaccgcca ggcggacagc
     7501 aaagctggtt attttctcac accagttctt tacgcggcag cggtgactgg ggcaccttac
     7561 cgggatggct actcgcaaag cgaagaatcc tgcggccaca cgcgggaaag gggccggcgc
     7621 caagcgccga cgtggcaagt tggccccggg caagagcgcg cggggcctgg atgctgccga
     7681 ggttgccatc gcaatcgata gccccgaggt ggccgccgtc gccacgctcg ttgcgcaggc
     7741 tggtggcgca ccaatcggcg cctaccacga tccgctgggg ggccgcccgc tcctgctcgc
     7801 ctccctgccg ctcacggccg tgcagccgac gccctttcag cgcgacctct ccccgacgca
     7861 cgccaagcgc ctggcggaga aaatcgacga gacggcagcc tttctcgatc cgctcatcgt
     7921 cgtgcgcggt gaggacggga ggctctggac acccaatggt cgccataggc tggcggccgc
     7981 caaggtcctc gggctgaagc agatcaccgc gctgatctct ccggacgagt ctctcgccta
     8041 tcgcatactc gccttgaaca cggagaaggc gcacaacctg cgcgatcgaa gcctggaagt
     8101 gattcgcatg gcgcgcaatc tggccaaaac gcagggcagc gcgcacgaat cgcagttcgg
     8161 cgcagagttc gagtccgcgg agctgctgac gctcggcatc gtgtacgaga agtcgccacg
     8221 tttcgccggc ggcgcctatg gcgcatttct gaagaaggtc gaccgcttca gcgagcgcac
     8281 gctcgccgcc agcctgcgcg agcgtgagag cttcgcggcg cgactgctgg agatcgatgc
     8341 ccaggccaaa cgggtgatgg ccgctctaca gacgcgcggc ttcaaatccc cgtacctgcg
     8401 caactatgtc gtggcgcgca tcaatccggt tcgtttccac aaggtgaaaa agggcgatac
     8461 gaagccgccg atgcccctcg gacaggcgct catccggatg gcggcggctg cgaaggcctt
     8521 caagatcgag tcggtatcca acagggacct cgcctgggtc gcggtgggcg cgggcagcga
     8581 ataggcgggt tcgatgcgat ccgatggcga ctcactgcgg cttgcgcgga gcgacatcga
     8641 ggctgctgga gtagctcagt ccgtacccgt acggaaacaa gggccgctcg gaaccctcac
     8701 cgtcggccgt cccactctga tgcggcgtgc ggggccacga gaagggcagc ttgccgcgaa
     8761 agtcgaagcg caccgagcca tcgggcgccc tgaagagcac atcggcaacc ccgccgccct
     8821 cgctgcccgg aagccatgcg gcgacgaaag ccgtcgaggc gtcgagctcg ggactcaccc
     8881 agagcgggcg gccggaaagg aacaccgaca ccacggggat cctgcgcgct tgcaggctgc
     8941 gcaacagctg cagatccgac ttgtcgccgg gactgaattg caccgtgggc cgatcgccgg
     9001 cggcctcggc gtacggattt tcaccgaaca ccacgatcgc cacatcgggc ctgacggtga
     9061 atgagccggt cgagctgagc tgcgcggtgc cgcctcctgc gacgaccgca gctcgaatgc
     9121 cctcgtagat cgactcgctg tgaggaaagt cctggttggc aaggcccgta ccctgccagc
     9181 gcagtgtcca gccgccgctc tgcttggcaa tgttgtcggc gccatcgccg gcgaccagca
     9241 cgcgctggcg tggtgacagg ggcagcacct ggtctcggtt cttcaacagc accagtgatt
     9301 cgcgcaccgc gcgccgcgcg agagcccggt gcgcgctcga gccgagcaga tcgaatcgcc
     9361 cggccatcgg acgcaacgag ggcttaccct cttccaacag atgcgcgcgc aacttgaccc
     9421 gcaaaacgcg tctcaccgca tcttcgaacc gcgcgggagg gatctcgctc gtgcggacct
     9481 gacgcagcgt gttgtcgaac agccccttcc agctatcggg cgccatgaac atgtcgaggc
     9541 cggcattgat cgccttggca cagctcacat tcgagcaccc cggaatctga ccttgcgcat
     9601 tccagtcccc gatgaccagg ccatcgaaat gcaggcgtcc tttgagtaca tcggtgatga
     9661 ggctgcgctg ggccgccatc ttggtgccgt gccagctcga atatgacgtc atgacggtct
     9721 gcacgcctgc ggcgactgcg ccgggatagc cggcgaaatc gatgtcccgc aactgcgcct
     9781 cgctggacag gttatcgccc tcgtcatgtc cgtccgtacc accaccgccg acaaagtgct
     9841 tggcggttgc gaggatgtgg gtggaatcca gaaactgagc ggtaccgggc tcgccctgca
     9901 gacccaggac catctgccgc gcgagctcgc gcacgagctg tgggttttcg gagtagctct
     9961 cgtaagtgcg cccccagcga acatcgcgag ccacggcgag cgtaggggcg aacgtccagt
    10021 ccaggccgat cgtgcgcacc tcgagcgccg tgacggcgcc gatctcgcgt agcagcgccg
    10081 gatcgcgcgt ggcgcccagc ccgatgttat gtggaaatat ggtggcgccc ggaatcttgg
    10141 cgacgccgtg gaccgcgtca ataccccaga gcgtgggtat ggggcggggt ccctggacag
    10201 ggtccacgga ggcctcatag aaccgatcgg cgagggccag ccactcacgc ggtggagcga
    10261 acacattgtt gccgggcgaa gaattcccgc cgttcagcac ggagccgagg ggaaattggc
    10321 gaaggtcatc gggtgtgatg tgcgcgatgt cggcctgcag cagctggccg acctgctgct
    10381 cgggccgcat ggccgcgagc agtcggctga tccgcgcctc cagatccgcg tcccgcgcga
    10441 tcgcaggcgg cacttgcggc cactcagcgg gatgaacgag tccctcgggt ggggccgcga
    10501 ggcgaccgcc ggcagcggac gtccccagcc cggtgcccag aaccaggact acactcgagc
    10561 acagcagagc gccacccggc gccctttgcc tgccgagccg tctcgtgctc acctccccgg
    10621 cacccttgcc ggcgggccct cgcatgcgat gggtccggcg ggcgcctgga ccatacagcg
    10681 atgagagtgc catcaacgaa gggccgacct atatgctgga gcaaacaaga acattcctcc
    10741 ctgcggagag tccagggctg cacgccaagc gactcgtaga gcctatcccc gtgcttggcg
    10801 atctgcccga tctgttgctg gtcgaacgca ctcgtggccg cgatacccgt gcgttcgaga
    10861 ccctcatgcg ccggtacaac cggcgcctgt accggatcgc gcgcagcatc ctcacgcgca
    10921 acgaccaggc cgaagaggcc gtgcgcggcg cctacctgcg cgcctttgcc aatctggacc
    10981 gctacgagcc ggccggtaaa tttggcgcat ggctggcccg gctagccttc aacgaggcct
    11041 tggctctgcg gcggcacctg gccaccgcgg gcagccccgc tgataaagtc gaacgcccga
    11101 cgccgaccgt gggctccaac gagacggctc tggagccggg cggaccgggt gccgcggaag
    11161 gccatactgt taactcgacc tcgagctccg cggcactcgt ctcatccgaa atggccagca
    11221 ccgagatgag cgagcagatg ctggagctgg caatcgatac cttgccggaa gtattccgga
    11281 cggtcctggt gctgcgcctc atcgaggagc tgagcggggt ggaggtagcg gtctgcctcg
    11341 ggctgaatga gaccacggtc agaacccggc tgttcagggc ccagcagcga ttgaaggtcg
    11401 atattgcgcg tcgcttgcgg gaaggccccg ccaacatctt cgagctgaca cccgagcggg
    11461 ccgaccgtat cgtctcgcag gtcttcgcgc gcctgcatac agccattcca accagcgagc
    11521 cgcgctgagc ggcccgctgg ctggcccggc agccatggct ccggcgccgg ggagtggatc
    11581 agcggatcag cttcgggccg ccggctgcgc cccctcggac gcggagcggg attcgctggc
    11641 ccgcgcgcgt gaggctgagg cgaactcctc gagcatcttg cggttgaagg ccggcaggtc
    11701 cgccggcttg cgactcgaca cgagcccctg atccacgacc acttcactgt cggtccagcg
    11761 ggctcccgcg ttcaccagat cggtcttgac ggagggccac gaggtgatgg atcgcccctt
    11821 cacgacaccc gcctcgacca gcgtccaggg tccgtggcaa atcaccgcaa cgggcttctt
    11881 tgcctcgaag aagctgcgca cgaacttcac ggcctgaggc atcgtgcgaa gctgatcggg
    11941 attggccaca ccgcccggca ggaccaacgc gtcgaaatcc tgcgcctcgg cttcctgcag
    12001 cggcacgtcc acttcgaaac gatccgcgtg atcgaagtga ttccagccct gaacctgtcc
    12061 cttggcaggc gagacgatct gcgtctcagc gcctgcctcg ttcagggcct tgcgcggctc
    12121 cgtcatctcc acctgctcaa atccgtcggc cacgagaata gcgaccttca atccgctcaa
    12181 tgttggcatc agcttctcct tgagtgtgcg ttcagatcgc cctgcgaggg cgaccggtag
    12241 acagcagcag acggcgtgcc gcgctgccag cgtcgagatc gagctgagcg tgcggccaag
    12301 caagagcatg catcgtgatc gcctccggtc tcgcaagcgc ttcaacagga cccaagcaac
    12361 aggcgcaatg tccgtgccga gagtgcacga ccgcccgccc acggcttgcg cagccatgtt
    12421 gagttcggag aactccgaag ttggtgttgc actgcgctgt gctcactgtc gcttgttggt
    12481 gacatcgggt cgcaggctga atcggcgctg aacgacatct caggtctcgg gcaggcggta
    12541 cctcaggcac gacgcttgca gtttgggact catgcccgac acgatgtggt tcgacgaaga
    12601 cgaactgcgg atcgaggcgc gtcgccgtgt gaagagcggc cgccttccct gccagccgcc
    12661 ccagtacatg tgggcgggcg ccggtgacgg ccagaggtgc tcgctgtgtg atcgggcgat
    12721 cgatgctcag cagatcgaat acgagctgga gttcagcacg caaccgagcg tcatccatcg
    12781 cttccatcgc atctgccatg aggcctggga gctcgagtgc atccgctgag agcctgacgg
    12841 cggcgcggac tgcggcaggc cgccgcactt gctgcagcgg ccagatgagc ggctgctgcg
    12901 gcagttcggc gctctattgc ctgcgagcat ccggcataat ggctgcttta tccgaacggg
    12961 cggaaacagc caatgaagtt actctgcagc ttgccgggct tgctggccgg gctcaccctg
    13021 acactcgcgc tgggctcagt gcaggcgaag gaaggcgatc acgaggcagg ccggcccgat
    13081 cacgccgcca gcgaggaatt tccctcgcca cgctatctcg aggatcaaag ccagagcacc
    13141 tccgccgcga tcaacgcacg cggtgcaaag atcgcctacc aggccgaggc cggcgtgcag
    13201 gtcgtctatc tcaaggatcc aaaagacgag gacccgccac cgccgcatga cgagcacaac
    13261 aatccgccgc ctcccccggt gcatgccacc atgtcgtacg tggcgtattt caagggcgac
    13321 aagcccgatc cccaccggcc catcaccttc ctcttcaacg gcggccccgg ctcctcgacc
    13381 gtatggctgc acatgggcgc cttcggtccg cggcgggtcg tgacggccaa tgattcccac
    13441 agtccggccg ccccctatcg catcgtggac aacgaataca gcctgctcga tgcaagcgat
    13501 ctggtgttcg tggatgcacc cggtaccggc ttcagccatc tgcgcggtcc ggacaaggag
    13561 aaaagcttct acggcgtgga cgaggatggg cacgcattcg caaatttcat cgtgggcttc
    13621 ctgtccaagc atggccgctg gaactcgccc aagtacgtct tcggggagag ttatgggacc
    13681 acgcgcgccg cgctgctcgc ctacatcctg cagggtgaga aggcggtcga tctcaacggt
    13741 gtgatcctgc tatcgcagat tctcaacttc gacgacaacg cggacggacc gcaattcaac
    13801 cccggcgtcg atcagcctta tgtgctggcg ctgccctcct atgcggcctc cgcctggtat
    13861 caccacaagc tgcccgagca gcccgcagcc ctcgagcctc tgctggcgca ggtggagcag
    13921 ttcgccctga ccgactactg gccggcgctc atggccggca accacctcag cccgcagcgc
    13981 cgcagcgaga tcgcagcgaa gctgcacgag tacaccggac tgcccaccga ttacatcgag
    14041 cgggccgatc tgcgcatcaa cgtcggtgag ttcgagaaaa acctcctggg aagcgagatc
    14101 accaccgggc ggctcgacag ccgtttctcc ggtcccacca tcgatccgct gagcaaggaa
    14161 gccgactacg acccgcagtc ggcctcgatc tcctccgcat acgtctcggc attcaacgag
    14221 tatgtgcgca cgacgctcaa gttcggcacc aacatgatct acaaggaggt gatcgacgtg
    14281 gagaagacct gggattacca gcatcagccg cccggatccc ccgtcaagct accgcaggca
    14341 ccgaatgtcg agctcgctct ggcgttcgcc atgaagacga acccgaacct caaggtcatg
    14401 atgaacggcg gttactatga cctggctacg cccttcttcg ccgcggtcta cgagctggag
    14461 catctgccga tccagccgga gctgaccaag aacatcgaga tgcacttcta cacctcgggg
    14521 cacatggtct atgcgcacga gccggatctg aaagccctgc acgacaatgt cgccgacttc
    14581 atccaccgga cggaggcatc cggtggtgcg gggacgcagt agttcatggg aagggctcag
    14641 ccgcggccga cgaaaggcat tcgggtcgcc atgaccgtca tgaacagcac gttggcctgc
    14701 agcggaaggc ctgccatgta gagcacggca tcggcgacgt gcttcacgtc gatgctcggc
    14761 tcagggcgaa gcgacagatc ggcctggagt gttccatggg tcatatgggc ggccagatcc
    14821 gtcgcggcat tcccgatatc gatctggcca caggcgatgt cgtatgcccg cccctcgagc
    14881 gcggtcgcct tcgtgagccc cgtcaccgcg tgcttggacg ccgtgtaggc gatgatgtgc
    14941 ggccgtgggg tagtcgccga caccgagcca ttgttgatga ttcgcccacc gcgcggctgt
    15001 tgcgtcttca tcagccgaaa ggcggcctgc gtgcacagga aggtgcccgt caggttcgtg
    15061 tcgatcacgc tttgccactg ctccagtgtc acttcttcca gcggcgcagc aggagcgccc
    15121 gtgccggcgt tgttgaacag gacgtcgagc ctgccgaact ggctggcaac ttgcgcaaag
    15181 agcgcagcga cggactggcg gtccgcgaca tccgctgcga tgacagaagc ctgggagccg
    15241 gcctccgcag ccgtggcctg cagactggcc agcgtgcgcc cggtgagcac caccacgtag
    15301 ccggccttgg ccagcgcgag cgccacaccc cgtccgattc cggagcccgc gccggtaacc
    15361 agcgcaatcc tgggattcat cgcagccctc ccgtcacgcc aagatgcgtg agccacgcca
    15421 gtcctgcccc ggtatcgccc cgaggacggt actcgcaacc tacccaacct gtgtaacctg
    15481 actcctccag cgcctgaaag atggccggat aggcgatctc gccctcatcc ggttccgccc
    15541 gcgacggaac cgccgcgatc tgcacgttgc cgagccaact cgtgagctgc cggagtttcc
    15601 tcagcacgtc gccctcgctg cgcgcgacgt ggtacacatc gaactgcagc ttgagattgg
    15661 gaagattcaa ctcctccagc agactcaccg cctcctccag cttcgagtag aaatagcccg
    15721 ggttgtcaca agaattcagc ggctcgagga gcagcgtcag cccgtggctg gccgccttgg
    15781 cggcggcgac gcgcaggttg tcccggaaaa ccgcacgggc tcgcaggcgc tcggccgagg
    15841 gaacgttccc ggccatcgcg tggatcgcgc gggcccccgc aatgcggcaa taggcgatcg
    15901 cctggtcgac gctcgcctga aagtgcagct cacggccgat gagcgcaccg agtccccgct
    15961 cgcccgcctc gaattggccg ggcgaggtat tgatcccgag cagcgtcagt tgattgcgtc
    16021 gcgcgacctc gccgagcgtc tcggcagcca cctcgtaagg ccagtgcatc tcgactgcgc
    16081 gaaagcccgc ttgcgcagcg gcctcgagcc tcgcaagtag cggcctgtcg ggccagagca
    16141 atccgagatt ggcagcaaac cttagcatcg actcacccgc tcgccctccc cgattgtatc
    16201 tcccgtggcg caagtcagca tcgaccgccg ccgggcattg acgtacgctg ctagcgggct
    16261 tcgtggaggg aacaggccat gagtggtttc tatgacatcg aggtcaaggc catcgacggc
    16321 acgccggacc tgctgggcag actgcgcggc aaagtctcgc tcgcggtgaa cgtggccagc
    16381 cgctgcgggc tcacgccgca atatgccggg ctcgaagagt tgcagagaga tctgcaggat
    16441 cagaacttca cggtcatcgg ctttccctgc aaccagttcg gagcccagga gcccggcacg
    16501 gagcagcaga tcgtgcaatt ctgccagagc cgttacgatg tgagcttccc gatgagtgcg
    16561 aagctcgagg tgaatggatc gggacgccac ccgctctacc agttcctgac atcagccggg
    16621 acaggcgtcg ccggcgatat cacctggaac ttcgagaagt tcctcatcgg acgggatggg
    16681 aaagtgttga agcgctatcc ccccccgacc aagccgcagg acagcggtct gatgcaggat
    16741 atcgccgacg ctctctgaga gcgtcgatca cgacaaggat ccaggagacc cacaccgtgg
    16801 catctgacag ttgcctttcg cggcgcagtc tgctcggaaa gggcctgctc gccgccctgc
    16861 tgccgctcgt cctggcgcgc agcgatcccg cccgtgcaaa ggatgcggcg ttactcagcc
    16921 cgagcgatcc ggcagcgcag aaactgaagt acaccgagga ggccgccaaa accaagggcg
    16981 taccgcccgg aaactcgtgc gcaaactgcg ccctctacca gggttcctac agctccaaac
    17041 agggcccctg tcagctcttc cccggcaagc aggtgcgggc ttctggctgg tgctcctcat
    17101 ggaccgcaca gatgtgacac cgcacgcgcg gccggtctca tcggatccat tggaaacaaa
    17161 cgccgcagtt gcgacagtcc cgatcggata gcggacggcg aagtgccgcc gtaccccagc
    17221 acgagcccgc gcctcgatct gctgccgacg caatacaccc gaagcgagcg aacatcgagc
    17281 ccgagctcgc gcgcctgcgc ggcaatgttg tcccaatcca gcgcttggcg ctccagggcg
    17341 gtcaggtgca tgcctccgct ggcgggaatc ggctcgagcc aaggggcaaa ctgcccctgc
    17401 aggacgctca ccagcaaggt gcgccgctcg ccatagagct tgcgcatctt gcgcacgtgg
    17461 cgcgccatgt ggccctcgcc gatgaacgcg gccagggcat cctgctcgag caccgggcta
    17521 tgccagtcgg cgcactgctt ggcagcgatc agtgcatgac gagcccagcg gggcacgacc
    17581 acgaacccga gccgaatgcc gggaaaaagg ctcttggaga acgtgccgac atagaagacc
    17641 gacccggatc gatcgagtgt ctgcagcgca tccctggggg tggacccgaa acgaaactcc
    17701 ccgtcgtaat cgtcctcgat cacgacagcg ccgctgcggc gagcgaactc gagcagctgc
    17761 gcgcgccggc gcatcgacat ggccacaccg agcgggaact gatgtgaagg ggtcacgcag
    17821 atcaggcgcg tctcagcggg caggctgtcg accaccagcc cttcggaatc caccgctgcg
    17881 gggatgatcc gtgcaccatt ggcgatgaat gccgcccgca acgggggata acctggatcc
    17941 tcgaacgcaa cgctcgtctc cccaggcgtc accagaattt tcgcgagcag ctcgaatgcc
    18001 tgttgcgtgc cgctggtcac gacgacatcg tccggctcac atgcgaccgc gcgagcaaag
    18061 gaggcatggt gagcgattgc ctctcgcagg gcggtgcgcc cttgcgcctc gacgaaggca
    18121 gcgggtgatt tggccaatgc gcgcagcgag cgtgcgagca ggcgcctcca gatatgaaaa
    18181 ggaaaggctt ccgcatcagg aaccccgatg cggaagggag cattcccggg tgccgccgaa
    18241 gcccagctgg tcccgagatc ggccggcggg gcgcgccaga atgcattcag ccgggggtcg
    18301 gccgcaggca gatgagccgt ggccgctcga gtgtggaccg gcaaggagat ggcgacatag
    18361 gtcccggcgc catggcgggc gcagagataa ccttcactga ggagaagatc gtacgcgccc
    18421 atgaccgtgt tacgggacac accccacgag tgggccagca gcctcgtgga gggcaggcgc
    18481 aggccgggct tgaggcggcc gtcgagaatc gccgcgcgca attcccgatg caaagcgctc
    18541 atgacgctgc cggagccgcg cggcggcaga gtgatggaga gctcaagcat gggacacccg
    18601 atgtctgcag tcgttcgtcc acaggtgcag cacggcatag ccgcgccacg gccgccagca
    18661 ctctgcccgc ttcgacagtt cgcgggcact cagcagcgtg ccgccattgc ccgctgcccg
    18721 acgcacgatg agatcgccgg cgaggaaggc atccggctcg cccagcgcgc gcaacacgac
    18781 atactgagcg gtccagtcgc cgagccccgg caaggaagcc agcacggcgg ccacctcctc
    18841 cggcggcgcg ttgaaatcga gccggccctc gatgagcgca cgcgtcatgg cgcgcagagc
    18901 gcctacacgc ggcgccgtga cgccgagtcc ctcgagctcg gcggccgcga cctgctcggg
    18961 cgtgggaaac aggtgagtca ggcgatcggt gccgcaatcg attcgtgatc cggcgcgcgc
    19021 caccagccgg ctgacgaagg tccgtcccgt cgcgacactg acctgctggc cgagaatcgc
    19081 ccgcacggcg cattcaaacg gatcccacgc acccgggatg cgcaatccgg gctggtttct
    19141 gaccagcggc gcgagcaggg catccgaccg cagtacctgc gcgatccgcg tcgggtcggc
    19201 cgcgagatcg aacgtgcggc gcgcgattcc ggacagctgc aacagcgctg cggccggtgc
    19261 tccccgcacg cgaaactcga gagcatcggc ctccggtagc gggcggactc gtacgaccgc
    19321 cggcccgctg ctcaaaccaa cagtgcgcgc atagccgtgc tcgtccacgt gctccacgcc
    19381 cgggagggcg cgcgccgcga gaaacttgcg cacctgcatc cagtcgtacg gcggccggta
    19441 cgccagtcgc aacgcgactt catcgcccgg ctcgcaacgc gccggttccc ccgcccgcct
    19501 gcgcagctgt cggggtggcc gctcataagt ggcttggaaa gccgagttga agcggcggat
    19561 gctggagaag cccgaggcga gcgcgatctg ggtcacgggc aggtccgtct catccagcag
    19621 ccgcttcgcg aagtgcagcc ggcgggtctg ggcgaccgtg atgggcgagg ccccgacatg
    19681 gtgcgagaac agccggccca gatggcgtgt gccgacgcct acccgcgcag ccagcgcgct
    19741 gacggaatcc gaatcgagtg ccccggagtc gatgagccga agtgcgcggc ggaccacggc
    19801 ggaagtcccc atccacgcag gcgtgcccgg tgccgcctcg ggtcggcagc gcaggcaggg
    19861 gcggtagcct gcttccgccg cagcggctgc cgtggcgtag taacggacat tggtgctctt
    19921 cgacggcggc gaaggacaga ccggccggca atagatcccg gtcgaggtca ctgccgtgaa
    19981 gaacttgccg tcgaagcgcg gatcgcgact caggcgggcg cggtcgagcg cgcgccgatc
    20041 caggctgagg gtgtcgggca tgacggccaa gatacccggg cgcagccgct tcgactagcc
    20101 attttcggac atggttgcgg ggtttaccgc ccgcgaccca gtgtcgtata cgctgtcggt
    20161 gcgaatgcac cgctcgagcg cggtcatcga ggtgtcatga gtgccatcat cagcgcgttc
    20221 ctgctgacct tcgcgggcct gtttccgatc gtcaatccga tcgaggcagc gccgttcttt
    20281 ctcgcgctga cgccgaacct cgcgccggcg gagcgcaacg cactcgcccg ccggatcgcc
    20341 gtgaatggtt ttgcgcttct gctgggttcg atggtgctcg gcccctggct gctcgagttc
    20401 ttcggcatcg agctgccggt ggtgcgcatc gcaggcggct tcgtcgtcac cgcgctcggc
    20461 tggaaactgc tgacgcagga ggactggtcc gatcacggct cggcgccgct cgaccccacg
    20521 agcgggcggc ggatcggcac gttttatccg ttgacgatgc ccttgaccgt ggggccggga
    20581 tccatctcgg tcgcgatcac cctcggcagc aagaagcccg ccacaggatt ccgtctggcg
    20641 gatgtcgcgc tgcatgccgt gggcgcgctg accggcgtga tcgcgatcgc cgccagcatt
    20701 tacattgcgt accggttctc gggcaacatc gcgcggctgc tgggcgcaac cggactggag
    20761 gtcgtggtgc gcctctcggc gttcatcctc atgtgcatcg gcatcgagat cgtgtggaac
    20821 ggctacagca cgctcattgc gcactgattg ccgcgcgcgg cgctccagcg ccggggccgc
    20881 gacgccgatg ctccagatcc ctcaagaacg cctgctggtc gcgcggggaa atcaacatgg
    20941 agcgcccgct tccataatcg atccgcagcc ggtcgagcga cagggcaggc cccgagcggc
    21001 tgtcgcgcgt ggccgtcacc gagtggatct catccagcgc aatcctccag cgaaagggcc
    21061 cgcaacgaac ctgcagcatc gttgcatcca gggtgtaact ggttcccaac agcgtccaca
    21121 tcgcgaagag gaccaccaga aagaagacaa tacgcaccgc ggccgacaat gcatgcggtt
    21181 gcatcaccga gacgacgcgg gcggtcacga cgaaaatgac cggtagtgcg agcgcaggca
    21241 gcaggatggg ccagtcaacc ttggatgggt aagttctcga cacgcccccc gcctccctca
    21301 cgccttgggc gattcggccg cgaagcagtc gctgacgatt cgttgcgcct cctcgaggat
    21361 gcgctccagg tgctccttgc cgagaaaact ttcgccgtac accttgtaga tgtcttcggt
    21421 accggaaggt cgcgcggcga accatccgtt gcgggtcatg actttcagtc cttcgat
//
LOCUS       DASRHN010000158         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_53348, whole genome shotgun sequence.
ACCESSION   DASRHN010000158 DASRHN010000000
VERSION     DASRHN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53348"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFB37_06105"
     CDS             complement(<1..507)
                     /locus_tag="VFB37_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZO22062.1"
                     /translation="MNFLRPRVIPCLQFAGGELVKTRRFKQPLYLGDPVNAVKLFNDL
                     ECDELVVLDIRATLEKREPEYALIEEFASEAFMPLAYGGGVRSIEQIRRILSIGIEKV
                     VIGAAAADGQLIRSAAAVFGSQSIVVAIDVHKTLLGRRDVWVRSATRNLGIDPQAYAL
                     QMQEFGAGE"
     gene            complement(504..1106)
                     /gene="hisH"
                     /locus_tag="VFB37_06110"
     CDS             complement(504..1106)
                     /gene="hisH"
                     /locus_tag="VFB37_06110"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZO22063.1"
                     /translation="MSILNYGIGNTGSISNMLRKLRVSAEVISTPAAVVAARRLVLPG
                     IGAFDACVEALQNSGLREPVLEFAAAGRPLLGICVGMQMLTRGSEEGCLPGLGLIPAY
                     THRFTEQDGLRIPHMGWDHVRWLVPDHPLGRHLLQDSRFYFVHSFRVTCELPTNCLAV
                     CSYGGEFAAAIGSGNITGVQFHPEKSHRFGLQLLSNFATT"
     gene            complement(1103..2416)
                     /locus_tag="VFB37_06115"
     CDS             complement(1103..2416)
                     /locus_tag="VFB37_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22064.1"
                     /translation="MATALASIVLPLDYIHVLCLIGVLLYVWGREPARLLLTPLTLLS
                     FFILYGVGNIIYFLGEETVPEVRRAVTLSMILMWIGIIAGIELARALAPGLSAQARRI
                     ARLWSTTRMHDDGSGDQLLAMVGAAVALFMLLMFLGLGKPSQILTFLSIGTAHEKLKY
                     RLEFGSEGGYLYRTLAASVAPFLSFLLLSKAACTGRRYLLLIGGAVCLAAFAGKLGTF
                     EKVPWLVYILQLMIVFQARKRLEIGVGRILMFLIILLGGVVAATLIAIPDLQSTDLLS
                     WLGFRFFQVNSEVIYQTFYVYPEHLPHTWGMNIGLVHALLGTGELQSAHTQVANFFGA
                     EGATFDAFFIADAWVDFSYAGVAVMAVLVGLVLKMVDMFVLSRGKTPLSVALLASGMY
                     GLFQLQVTSAFTAFLSGGLVLIPLLVLGAEGLMNDLTRGRLQWQR"
     gene            complement(2401..3270)
                     /locus_tag="VFB37_06120"
     CDS             complement(2401..3270)
                     /locus_tag="VFB37_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZO22065.1"
                     /translation="MNDVAREPVQYEVSLVAMAQFLWRNILLIGGCMVLFGGAATALV
                     IMATPKWRSEVVFSPASSSGGLGNMGGQLGGLAAIAGIELGGASKKTDEELAYLRSRG
                     FTAQFIQRHDLLPVLFASRWDAQNKRWTGEPPTMGEAVKRFSNQVRQISEDRRTGIVT
                     MSILWRDRFVAAQWANALLAEADDALRKRAIAELTRSIDYLKAESSDASVVEVQAAVY
                     KVMESELKDAMLARTRDAYAFKVLDPAVAADPKDIASPKKVLDIGIGCAFGLLVGILC
                     AAVIRRGPAWPRR"
BASE COUNT          661 a         1101 c         1007 g          546 t
ORIGIN      
        1 ctctcccgct ccgaattcct gcatctgcag cgcgtaggct tgcggatcga tgccgaggtt
       61 gcgggtggcg cttctaaccc agacgtcccg gcgcccgagg agcgttttgt ggacatcgat
      121 cgcgacaacg atgctttgcg aaccgaacac cgcggccgcc gaccggatga gctgcccatc
      181 cgccgccgca gcgccgatca cgaccttctc gatgccgatg gagagtatcc gcctgatttg
      241 ctcgatggac cgcacgccgc ccccgtaggc gagcggcatg aaggcttcgg aggcgaattc
      301 ctcgatcagg gcgtattcgg gctcgcgctt ctcgagcgtg gcccggatgt ccaataccac
      361 cagctcgtca cactcgaggt cgttgaacag cttgaccgca ttcaccggat cgccgagata
      421 cagcggttgc ttgaagcggc gggtcttaac cagctcgccg cctgcgaatt gcagacacgg
      481 aatgactcgc ggacgcagga agttcatgtt gtggcgaaat tgctgagcag ctgcagaccg
      541 aagcggtggc tcttctcggg gtgaaactgc acccccgtga tattgccgct gccgatggcc
      601 gccgcaaact ccccaccgta gctgcatacc gccagacaat tcgtcggcag ctcgcaggtg
      661 actcgaaagg agtgtacgaa gtaaaaccgg ctgtcctgca gcaggtgccg gccgagcgga
      721 tgatcgggaa ccagccatct cacgtggtcc cagcccatgt gagggatgcg caggccgtcc
      781 tgctcggtga agcgatgggt gtaggcaggg atcaacccca gccccggcag gcagccttct
      841 tcgcttccgc gcgtcagcat ctgcatgccg acacatatgc ccagcagggg ccggccggcc
      901 gctgcaaatt ccagcaccgg ctcgcgcaat ccggagttct gcagggcctc gacgcacgca
      961 tcgaacgccc ctatgcccgg cagcaccagg cgccgcgccg ccacgaccgc ggcgggcgtg
     1021 gagatgactt ccgcgctcac acgcagtttg cgcagcatgt tggatatcga gccggtgttg
     1081 ccgataccgt agttgaggat gctcatctct gccactgcaa tcgcccgcgt gtcagatcgt
     1141 tcatgagccc ctcggcgccg agcaccagaa gcggtatcag gaccagaccc ccggagagaa
     1201 atgcggtgaa agccgaggtc acctgcagct ggaagagacc atacattccg ctggcaagca
     1261 gcgcgacgct cagaggagtc ttgccgcggc tcagcacgaa catgtccacc atcttcagca
     1321 ccagcccgac cagaaccgcc atgaccgcga cgcctgcgta ggagaagtcc acccacgcat
     1381 cggcgatgaa gaacgcatcg aaggtggctc cttcggcgcc gaagaaattc gccacctggg
     1441 tatgcgcgga ctgcagctcg ccggtgccga gcagcgcatg caccaggccg atgttcatgc
     1501 cccaggtgtg cggcaggtgc tcggggtaga catagaaggt ctggtagatc acctcgctgt
     1561 tgacctggaa gaatcgaaag ccgagccagc tcagcaggtc cgtgctctgc aggtccggaa
     1621 tcgcaatgag cgtggccgcg accactccgc cgagcaggat gatcaggaac atgaggatcc
     1681 gccccacgcc gatctccagt cgcttgcgcg cctggaatac gatcatgagc tgcaggatat
     1741 agacgagcca cggcaccttc tcgaacgtgc cgagtttgcc ggcgaatgcg gccaggcaca
     1801 ccgcgccgcc gatgaggagc aggtagcgcc gtccggtgca cgcagccttg ctcagcagca
     1861 gaaagctgag aaacggggcg accgaggcgg ccagcgtgcg gtacagatag ccgccctcac
     1921 tgccgaactc gagccggtac ttcagtttct cgtgtgctgt cccgatcgac agaaacgtca
     1981 gaatctgtga gggtttgccc agcccgagaa acatcagcag catgaacaac gccaccgcag
     2041 cgccgaccat ggccaacagc tgatccccgc tgccatcatc atgcatccgg gtagtgctcc
     2101 agagccgcgc gatccgcctg gcttgcgcgg acagcccggg cgccagtgcg cgagccagct
     2161 cgatgcccgc gatgatgccg atccacatca gaatcatgct gagcgtgacg gcgcgccgca
     2221 cctcgggaac cgtctcctcg ccgaggaaat agatgatgtt gcctactccg tataggatga
     2281 agaaggacag cagcgtgagg ggtgtcagca gcagccgggc aggctcgcga ccccagacgt
     2341 agagcaggac gccaatgagg cagagcacat ggatgtaatc cagcggcagc acgatcgagg
     2401 ctagcgccgt ggccaagccg gaccgcgtcg gataaccgcc gcgcagagga tacccaccaa
     2461 aagcccgaac gcacagccga tcccgatatc cagcaccttc ttcgggctgg cgatatcctt
     2521 ggggtccgct gctacggccg gatccaggac cttgaaggcg taggcatcgc gagtgcgcgc
     2581 cagcatcgca tccttcaact cactttccat gaccttgtaa acagctgcct gtacctcgac
     2641 cacgctcgca tcggaggact ccgccttcag atagtcgatg ctgcgtgtca gctcggcgat
     2701 ggcccgcttc cgaagtgcat cgtccgcttc cgccagcagc gcattggccc actgggctgc
     2761 tacgaagcgg tcgcgccaca ggatcgacat cgtgacgatg cccgtgcgcc ggtcctcgga
     2821 gatctgccgg acctggttcg agaacctctt cacggcctcc cccatggtgg gaggctcgcc
     2881 cgtccatcgc ttgttctgcg cgtcccatcg cgatgcaaac agcaccggca gaaggtcatg
     2941 tcgctggatg aactgagccg tgaacccgcg cgagcgcagg taggcgagct cttcgtcggt
     3001 cttcttgctc gccccgccga gctcgatgcc tgcaatcgcc gccaggccgc ccagctggcc
     3061 acccatgttg cccagacctc ccgagctgct cgccggtgag aaaacgacct ccgagcgcca
     3121 cttcggcgtc gccatgatga ccagcgcggt cgcggcgcct ccgaacagca ccatgcaccc
     3181 accgatcagg aggatgttgc gccacaggaa ctgcgccatg gccacgagac tgacctcgta
     3241 ctggaccggt tcccgggcaa cgtcgttcat gggtgcccct cgcaaagatc gcaaagtgcc
     3301 gcgacgatcc gtcgc
//
LOCUS       DASRHN010000159         6486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1373203, whole genome shotgun sequence.
ACCESSION   DASRHN010000159 DASRHN010000000
VERSION     DASRHN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373203"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1053
                     /locus_tag="VFB37_06125"
     CDS             256..1053
                     /locus_tag="VFB37_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22066.1"
                     /translation="MKTNIQSFPSFPELEALLRLQERLLHVSRLATIGEMSDGIAHEL
                     NQPLCAVANYAQACDRLLALPDPDIQEVRESLQEITAQALRAGEVIRRLRNLARPQQA
                     CHELASVNPLIAELTDLIQSDAKHHQVRYRFAPGPDLPNVWVDRPQIQQLLLNLVRNS
                     IEALAEVPPEAREVLVRSVCTATGDVEITVSDRGPGVSEGIAPHLFAPFCTTKSAGTG
                     LGLAMSRTIARANSGTLDYRPNVPKGACFTLTLPAAQPDRAEASMRV"
     gene            1189..1842
                     /locus_tag="VFB37_06130"
     CDS             1189..1842
                     /locus_tag="VFB37_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22067.1"
                     /translation="MKDRAPVISVVDDDAAARSSLRLLLKSLGLAAVAYDSAASFLAS
                     YDPEQPGCLLLDIRMPGMSGLELQQQLNQRGTIAPIIFITGHGDVAMAVEAMQHGAFD
                     FLQKPFRDQDLLDRVQRALAKDREIRAELRGHESIRQRLDSLTAREHDVLNLVTDGAP
                     NKIIAHKLGISQRTVEIHRARVMEKMSADSLAQLVHMTLIAGGAAPDAKDANARRAL"
     gene            1940..3505
                     /locus_tag="VFB37_06135"
     CDS             1940..3505
                     /locus_tag="VFB37_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO22068.1"
                     /translation="MISTLSSELVRSALDSSPDAMVLVDAKGLIVFANQQLSALFGYT
                     RDQVRGQSIELLIPERFRGRHPGHRATFAAEHRVRPMGVGLELFARRQDGSEFPVEIS
                     LSPINDTAAGGALVAAAIRDVTERKRVQQELIQAREAAERERQIADAARETADRANQA
                     KSRFLATASHDLRQPLQSLALLNGTLRRLVDHPDALEALTYQDQAIEAMSRLLNALLD
                     ISKLESGAIRPAPVDFTVTSLFKELEQEYTAVAANKGLQLHVSATPKSVHSDPALVGQ
                     ILRNLLSNAVKYTRAGSVRLVSLSSPGAVRIEVIDTGIGIPADQLQFIYDEFFQVGVP
                     TNSSRDGYGLGLSIVHRLVRLLQLKLEVRSEVGQGSVFALELPQGRASTVASTARATL
                     AQSARQASAPQLLLVEDDPAVRDATRMLLKVEGYRVLTAGSLAEARSAAQQNPQLDLL
                     VTDYHLSNGETGAQVITALREELGQHLKAVLITGDTSSAMKELQRDERLRLARKPIRA
                     EELLALLKSLLAP"
     gene            3619..4608
                     /locus_tag="VFB37_06140"
     CDS             3619..4608
                     /locus_tag="VFB37_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZO22069.1"
                     /translation="MRSVRRILVAIKDYEAKTAPALIKAGQLAKALGASIEIFHGIAS
                     PLYVDGYTMPSVELPQIEHTFRARILAELEKMATRLRREGVKVTTAAEWDFPVYEAIV
                     RRAHQIKADLIVADQHLGRHTAAGLLHLTDWELLRLSPVPVLLVKAPGTYRNPVVLAA
                     LDPGHTYAKPAKLDQRILAASATMAHALRGTQHVMHAYVPFPFAADPDTMVSQATMDR
                     LEAEASTAAQKALDRELRSRRIPRAQRHLVGRHPSDAIPQTARETHSSIVVMGAISRS
                     GLKRLFIGNTAERTLDLLPCDVLIVKPAQFTNRVPRARRGARVVAVTSTALPL"
     gene            4702..5835
                     /locus_tag="VFB37_06145"
     CDS             4702..5835
                     /locus_tag="VFB37_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO22070.1"
                     /translation="MRTPDTFAGLRSAEEFQALLDAAVDGIVLIDHTGRVQAFNRAAE
                     RLFGYLAEEVLGSNVSKLMAEPDQSDHDRHLARYVKTREPHVIGRGREVEARRKDGSI
                     FPVFLSVGVVDGAEPPRFVGFIQDISYRRRAEEETHRLQERLTHVSRLATVGEMSAGI
                     AHELNQPLTAVANYAQACDRLLGLPDPDIEEIRDALKQITAQAVRAGDIIRRLRTLAR
                     NDVMRREATDINALIRELTELIQLDAKTHEVQYRLDLTATLPPVDVDRAQLQQVVLNL
                     VRNAVEALSESPSSPRQVAVRTALLPEGDVEIAICDNGPGVSPAVAAHLFDPFCTTKP
                     NGTGLGLAISRTIVKAHQGALEYRPNLPSGACFTVRLPLVTQD"
     gene            5839..6465
                     /locus_tag="VFB37_06150"
     CDS             5839..6465
                     /locus_tag="VFB37_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO22071.1"
                     /translation="MKERSPIVFIVDDDEAVRSSLRLLLKSVGLVPNALASAREFLEK
                     YDPAQPGCLVLDVRMPEMSGLELQEQLNRQGAVIPVIFITGHGDVPMAVEAMQAGAFD
                     FLQKPFRDQDLIDRIQKALEKDRANRAVLTERTLIRERLESLTPREREVLTMVSSGKA
                     NKVMAADLGVSQRTVEIHRARVMEKMGASSLAQLVRMVMDLGEATPGS"
BASE COUNT         1236 a         2137 c         1995 g         1118 t
ORIGIN      
        1 aagcggcaca ggttgctgcc gaccaaaccc gcgccacctg tgatcagaac caacggctgt
       61 gtcatcgtgc cctcgtttac gttgcacgcg aggatcgcat gccgggcacg aagtccagta
      121 tccgcacagt ccggaccgat atacgggttt tccgagagag atgcccgtca cactcgctgc
      181 cgcagttcgc ccgagcgatg ttgagtgcgt gcaagtccgt atggcgagcc ccgcaatcgg
      241 aggcatgatg cgctcatgaa gaccaacatc cagagcttcc cctcgtttcc cgagctggag
      301 gcgctactgc gcctgcaaga acggttgctg cacgtatcgc gcctcgcgac catcggcgaa
      361 atgagcgatg gcattgccca tgagctcaac cagccactgt gcgcggtggc caactacgca
      421 caggcgtgcg atcggttgct cgccctgccg gacccggaca tccaggaggt tcgcgagtca
      481 ttacaggaga tcacggcaca agccctgcgc gccggagaag tgattcgtcg cttgcgcaac
      541 ctcgcgcggc cacagcaagc ttgtcatgag ctggcctctg tcaatccgct gatcgcggag
      601 ctcaccgatc tcatccagtc cgatgcgaag caccatcagg tgcggtaccg cttcgcgccg
      661 ggtcccgact tgccgaacgt ctgggtcgat cggccgcaga tccagcagtt attgctcaac
      721 ctggtgcgca actcgatcga ggcactggcc gaagtgccgc cggaggcacg tgaagtcctc
      781 gtccgcagcg tatgcactgc gacgggagat gtggaaatca ccgtcagcga tcgcgggccc
      841 ggcgtttcgg agggcatcgc cccgcacctg ttcgccccct tttgcaccac gaaatcggcc
      901 ggcacggggc tcgggctcgc catgagccgg accatcgccc gggccaactc gggcaccctg
      961 gattacaggc ccaatgtccc taaaggagcc tgcttcacac tgacgctccc cgcggcccag
     1021 cccgaccgag cggaggcttc gatgcgcgtg taagccacac ggctttgtga ggtattgcgg
     1081 taaatacgta cggcgctgcg gacaggtccg tattggccac tatgggcgat gcagcgaaac
     1141 tcttcccacc ctgaagcacg cccgctctca tgcggtggag aaagagccat gaaggaccga
     1201 gcccctgtca tctccgtggt cgatgacgac gccgcggccc gtagttccct acgcctgctg
     1261 ttgaagtctc tcggcctggc ggcggttgcg tacgactccg cagcatcgtt tctcgcaagc
     1321 tacgatccgg agcagccggg ctgcttgctg ctggatatcc gcatgccggg catgagtggc
     1381 ctggagctgc agcagcaact caatcagcga ggcacgatcg cgcccatcat cttcattacg
     1441 ggccatggag acgtcgccat ggcggtcgaa gccatgcagc acggcgcctt cgacttcctg
     1501 caaaagccgt ttcgcgacca ggacctgctg gatcgggtgc aacgagcgct cgccaaggat
     1561 cgcgaaatcc gcgctgagct gcgcggccat gagtcgatcc gacaacgcct cgactccttg
     1621 acggcgaggg agcacgacgt cctcaacctc gtgaccgacg gcgcgcccaa caagatcatt
     1681 gcccataaac tcggaatcag ccaacggacg gtcgagatcc atcgcgcgcg ggtgatggag
     1741 aaaatgagcg cggactcgct cgcccagttg gttcacatga ccctgattgc cggcggggcg
     1801 gccccggatg caaaggacgc gaatgcgcgc cgagccctct agcgcgctca ctcccgatct
     1861 cgaccgcgtc gagcccgaat tgccagcccc gaaaccggat gcgaagctag cgtcccaaga
     1921 acgggatgac tggtcctcga tgatctcgac gctgtcctcg gagttggtgc gcagcgcgct
     1981 cgactcctca cctgatgcca tggtgctggt ggatgccaaa ggcctgattg tctttgcaaa
     2041 tcagcagctt tcagcactat ttggctacac ccgcgaccag gtgcgcggcc agtcgatcga
     2101 gctgctcatt cctgagcgct tccggggccg ccatccagga caccgagcca cgttcgccgc
     2161 cgagcatcga gtccggccca tgggggtcgg cttggagctg ttcgctcgtc gccaggacgg
     2221 ctcggagttt ccggtcgaaa tcagcctgag ccccatcaat gacacggcag ccggcggggc
     2281 actcgtcgcc gcagcgatcc gcgatgtcac tgaacgcaag cgcgtgcaac aggagctcat
     2341 ccaagctcgt gaagccgcgg agcgtgagcg acaaattgcc gatgcggcgc gcgagaccgc
     2401 cgatcgcgcc aatcaggcga agagccgatt cctggccacc gcgagtcatg acttgcggca
     2461 gcctctgcaa tcgctggcac tgctcaacgg cactctgcgc cgactcgtcg atcaccccga
     2521 tgcgctggaa gcgctgacct accaggatca ggccatcgag gccatgtcgc ggctgctgaa
     2581 cgcgctgctg gatatcagca agctcgaatc cggagccatc aggccggcgc cggttgattt
     2641 caccgtcacc agccttttca aggagctcga gcaggaatac acggccgtcg cggccaacaa
     2701 ggggctgcag ctccacgtgt ccgccacacc caagtcggtg cacagcgatc cggcactggt
     2761 gggccagatc ttgcgcaatc tcctgtccaa cgcagtcaag tacacgcgcg ccggcagcgt
     2821 gcgcctcgtc agcctcagta gccctggagc ggtgcgcatc gaagtcatcg acacgggtat
     2881 cgggataccc gccgaccagc tccaattcat ctatgacgag ttcttccagg tcggcgtccc
     2941 gaccaacagc tcgcgggacg gatatgggct cgggctgagc atcgtgcacc gattggtgcg
     3001 tctgctccaa ctgaagctgg aggtccgctc ggaagtcggc caaggctcgg tgttcgcatt
     3061 ggagctgccg cagggccgag cctcgacggt agcctcgact gcacgggcca ccctcgcgca
     3121 aagcgcacgc caggcctcgg cgccccagct gttgctggtg gaggacgatc ccgccgtgcg
     3181 cgatgcgacg cgcatgctgc tgaaggtgga aggttatcgc gtgctcacgg cgggctcgct
     3241 cgcggaggcc cgatcggcgg cgcagcagaa tccgcaactc gacctgctcg taacggacta
     3301 tcacctgagc aacggcgaga ccggcgcgca agtgatcacg gctttgcgcg aagagctcgg
     3361 ccaacacctc aaggcggtgt tgatcaccgg tgacacgtcc tccgcgatga aggagttgca
     3421 acgggacgag cggctgcggc tcgcccgcaa gccgatccgc gccgaggaat tgttggcctt
     3481 gttgaaatcc ctgctcgcgc cgtgaggctc gcgtcatagg tattgttcct taagcatgga
     3541 tacgaatccc ttcgggcctc gcctggccgc aggatcggtc cacggttcgt tggaacaccc
     3601 gtcacaggag attcgatcat gcgctccgtc cgccgcattc tcgtcgccat caaggattac
     3661 gaggccaaga ccgcccccgc gctcatcaag gccggccagc tcgccaaagc gctcggcgcg
     3721 agcatcgaga tcttccacgg catcgcctcg ccgctgtacg tcgacggcta caccatgccg
     3781 agtgtggagt tgcctcagat cgagcacacc ttccgcgcgc gcatcctggc tgagctggaa
     3841 aagatggcaa cgcgcttgcg ccgtgagggg gtcaaggtca ctaccgcggc ggaatgggat
     3901 ttccccgttt acgaggcgat cgtcaggcgc gcccaccaga tcaaggccga cctcatcgtg
     3961 gccgaccaac acctcgggcg ccacaccgcg gcgggcttgc tgcacctgac ggactgggaa
     4021 ctgttgcggc tgagcccggt tccggtgctg ctggtgaagg cgccaggaac ctaccgcaat
     4081 cccgtggtcc ttgcggccct cgacccaggc catacttacg ccaaacccgc gaaactcgat
     4141 cagcggattc ttgcagccag cgccaccatg gcgcacgcac tccggggtac gcagcacgta
     4201 atgcatgcct acgttccgtt tcctttcgca gcggatcccg acaccatggt gagtcaggcg
     4261 acgatggacc gtctggaagc ggaggcgagc accgccgcgc aaaaagctct cgaccgggaa
     4321 ctgcgctctc gcagaatccc tagagcgcag cggcatctcg ttggccgcca tccctcggac
     4381 gcgatcccgc aaactgcccg cgagacccac agctccattg tggtcatggg cgcgatctcg
     4441 cgatcggggc tcaagcggct gttcatcggc aataccgccg agcgcacgct cgatctgttg
     4501 ccgtgcgatg tgctcatcgt caaaccggcg cagttcacga atcgcgtgcc gcgcgcccgc
     4561 cggggagcac gggtcgtggc ggtgacctcc accgcgctgc ctttgtaaga aggggcacca
     4621 ggggaatgga gtccctaccc tgatagactg tggcatatac ctacggacct gacacgggat
     4681 gggctgcata atcgctcgac aatgaggaca ccagatacgt tcgcgggact ccgttcagcc
     4741 gaagagtttc aggctctcct ggatgccgcc gtggatggga tcgtgctgat cgaccacacg
     4801 gggcgcgtgc aggccttcaa ccgggcggct gagcgcttgt tcggctacct cgccgaggaa
     4861 gtgctgggct cgaatgtcag caagctgatg gcggagcccg accagagtga ccacgatcgg
     4921 cacctggcgc gctatgtcaa aacgcgcgag ccccacgtga tcggccgagg gcgggaagtc
     4981 gaggcccgcc gcaaggacgg cagcatcttt ccggtctttc tgtcggtcgg cgtcgtggac
     5041 ggagccgagc caccccggtt tgtcggcttc atccaggata tctcataccg acgccgcgcc
     5101 gaggaagaaa cccacaggtt gcaggagcgg ctcacacatg tctcccgcct cgctaccgtc
     5161 ggcgagatgt cggcaggcat cgcacacgag ctcaatcaac ctctcaccgc cgtagcaaac
     5221 tatgcgcagg catgtgatcg cctgttgggc ctgcctgatc ccgacatcga ggagatcagg
     5281 gacgcgctga agcaaatcac cgctcaggcg gttcgtgccg gcgacatcat ccgcaggtta
     5341 cgcacgctcg cccgtaatga tgtcatgagg cgcgaggcta ctgacatcaa tgcgttgatc
     5401 cgggagctga ccgagctgat tcagttggat gccaagacgc acgaagtgca gtacaggctc
     5461 gaccttactg ccacgctacc gccggtggat gtcgaccgcg cgcagctgca gcaagttgtc
     5521 ctgaacctgg tcaggaatgc ggtggaagcc ttgagtgaga gccccagctc tccccgacag
     5581 gtggccgtgc gcacggcgct gctccctgaa ggggacgtcg agatcgcgat ttgcgataac
     5641 ggccccggcg tctcgccagc ggtcgccgct catctgttcg acccgttttg caccacgaaa
     5701 cccaacggaa ccggcctcgg cctggcgatc agtcgtacaa tcgtcaaagc gcaccagggg
     5761 gcgctcgagt atcggcccaa ccttccctcc ggtgcgtgct ttacagtgcg tttaccgctg
     5821 gtgacacagg actgatctat gaaagagcga tcgccgatcg tcttcatcgt ggacgacgac
     5881 gaggcggtgc ggagctcgct tcgcctgctg ttgaaatcgg tgggactcgt gcccaacgca
     5941 ctcgcctcgg cacgcgagtt tctggagaag tatgatcccg cccagccggg atgcctggtg
     6001 ctcgatgtgc gcatgccgga aatgagcggc ctggagctgc aggagcagct caatcgccag
     6061 ggcgcggtca tccccgtgat cttcatcacc gggcatgggg atgtgccgat ggcggtggaa
     6121 gccatgcagg ccggtgcgtt cgacttccta cagaagccct tccgcgacca ggatctcatc
     6181 gatcgcatcc aaaaggcatt ggagaaagac cgcgccaacc gcgcggtcct caccgagcgc
     6241 accctcatcc gcgagcgcct ggaatccctc accccgcgcg agcgcgaggt cctcacgatg
     6301 gtcagcagcg gcaaagccaa caaagtcatg gctgccgacc tcggcgtgag ccaacgcacg
     6361 gtggaaatcc accgcgcgcg cgtcatggag aaaatgggcg cctcctcgct ggcccagctg
     6421 gttcgcatgg tcatggatct cggcgaagcc acccctggca gctgagccca cgcccagctc
     6481 tcccaa
//
LOCUS       DASRHN010000160        22610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2195716, whole genome shotgun sequence.
ACCESSION   DASRHN010000160 DASRHN010000000
VERSION     DASRHN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22610
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195716"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..554)
                     /locus_tag="VFB37_06155"
     CDS             complement(84..554)
                     /locus_tag="VFB37_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22072.1"
                     /translation="MQTHSLATSQRLPFSAYEDVPDRALAPARLRILIVNEDFRSADS
                     IKYALQELGCFTTLTACSARKALQAAVDFAPSLALLDLELPDMSGFELARKLRAHPNS
                     GVRRTPLLAIAEREELGHTELTRAAGFIGCLTKPVQPVELSRILWKLRRWSSQV"
     gene            complement(557..958)
                     /locus_tag="VFB37_06160"
     CDS             complement(557..958)
                     /locus_tag="VFB37_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22073.1"
                     /translation="MTQSDVGNFPSSHARGAFITEGTGMNAITVVPEARSERSGSASD
                     GVLPDYRERRVIEALERAELKRVNLAEQHAPDTSADVRIRAWEKVHQLRMPSDPFHPA
                     LHAIAAATHLTLEDVRNEQQLRSSRRASASG"
     gene            complement(1207..2526)
                     /locus_tag="VFB37_06165"
     CDS             complement(1207..2526)
                     /locus_tag="VFB37_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZO22074.1"
                     /translation="MKRTQNQSVSLAGHIRRARNRILEEWRSRVAKDPQLTTASSITH
                     AQFMDSIPHVLEAFEKTLRADDGVGLTQANAEQRKGAAEHGLHRWQQGYDLRETILEW
                     AHLHRCILRELEEFARANPALDTEIVAEARDALVGLCGEGASESAARYTRLQQAEAAS
                     RMRDLEAALAHLRTLERSRGEILREAAHDIRGSVGVIATASAVLVRSRSGEAAPGSEK
                     VLERAVHSMQFMLGDLIDLARLEAGQDPLQITRFDAAQVLRELGESVRAVASERNLFL
                     KLEGPASLVVESDHVKLQRIVQNLVLNALKATEHGGVRLAWQAPDAGASRWTLMVQDT
                     GPGFDPERVGPLGRALYQATEQAHDSALEAAHAGDRSADPAAAPTLRSEDQHASTGSR
                     PGQGIGLAIVKRLCELLDAGLELETAPGRGTTFRVSFPMQSSREKLA"
     gene            2807..3187
                     /locus_tag="VFB37_06170"
     CDS             2807..3187
                     /locus_tag="VFB37_06170"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO22075.1"
                     /translation="MHRSRLCAILIDCKTTEVDEAARFWGKALGRPFDPTHPSSRGNY
                     RMLQTPPDEPIVEIQKVEHDSRVHIDIESDDIPAEVARLSELGAKVVTRLERWVVMEA
                     PTGQRFCVVRVQRPGFPKNANVWP"
     gene            complement(3244..4434)
                     /locus_tag="VFB37_06175"
     CDS             complement(3244..4434)
                     /locus_tag="VFB37_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZO22076.1"
                     /translation="MLHSRLVAALVILLCAVTIDSQAAASPAAGCIKETDRVRARQLG
                     IAPGVLAPGPLNAITDVQGVLVGHSTVVEGDSIRTGATAILPHGGNLYQNKVPAGVFV
                     MNGFGKSAGLSQVEELGEIEAPVVLTNTLAVPRGVEAVLDWTLSQPGNEKVLSANAVV
                     GETNDGTLNDIRGRALSSEQILQAIRSAHAGPVEEGAVGAGAGTVAFGWKGGIGTSSR
                     RLPEISGGWTVGVLIQSNFGGMLTVDGRRIGQELGKFYLKDELAGHSADGSIMVVIAT
                     DAPLSDRNLRRLARRATAGVARTGSSFSNGSGDYFIAFSTAQGGIRTSEKRANIGQFS
                     ELSNDAISPLFEAVAEATEEAIYNSMFMATTTRSHNVVTGKDVVIERLPLAPFLEGKK
                     IRCR"
     gene            complement(4599..6245)
                     /locus_tag="VFB37_06180"
     CDS             complement(4599..6245)
                     /locus_tag="VFB37_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO22077.1"
                     /translation="MRTNRLLIKLPVWAASLAVGSLGVHAVAADSRSPSAERTVLNSI
                     ADEGFNHSAIPETAEYLSDQIGGRMTNSPAMRRAERWTSGKFSEWGLKNVRTEAFDFG
                     RGWWIEASHIRMVSPRPVEMKGIAVAWTPPTNGTLTAPIIVAPLDSERDFADWKGKLA
                     GKIVLATWPAPQKDDSEPPFRRDSEADIAKLDKFEEPVFDPEQRKKRIEHFRFFAQRD
                     AFLAEEGALALITMSRSEGRLVHGEGYMYRVGQSPKLPAVELAAEDYRKLARLAKVGE
                     VKVEIENRVHYEDGDHNANNILAEISGGAAGSGYVMAGAHLDSWVAGDGAADNGAGSI
                     MVMEAARILVKVGAKPKRSIRFALWAGEEQGLLGSAAYVEKFLAVRPAPTDPALRDLP
                     PYFASETYPVRTLPGFNELDAYFNIDNGSGKIRGIYTEGNLNVVPIFREWLAPFAGMS
                     ADSVVARPTGSTDHVFLSRLGLPAFQFIQDPLDYESRVHHTDLDTYDHLRIDDMKEGA
                     VVLASVLLEAADADKPLPRKPVPTEPKLTDPFAYPDPARN"
     gene            complement(6480..7181)
                     /locus_tag="VFB37_06185"
     CDS             complement(6480..7181)
                     /locus_tag="VFB37_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22078.1"
                     /translation="MTQTSHQILIVEDDRAIREVLHTLLTQEGYGVIEAETAERGEIE
                     ARSHKPDLLLLDLGLPDNDGLSVVRHVRAWSSLPIIVLSARTLEEQKIASLDAGADDY
                     VTKPFSAAELLARVRAGLRRNARGSEQSSVLQFGTVRIDLPGRRAYGPQGEIHLTTLE
                     YRLLECLARHTGIVVRQQQLLRQVWGPDRVSDARGLRVCIKNLRDKLEPVPQRPRYIL
                     TEIGIGYRLCIEESR"
     gene            complement(7178..9871)
                     /locus_tag="VFB37_06190"
     CDS             complement(7178..9871)
                     /locus_tag="VFB37_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZO22079.1"
                     /translation="MADPRPDPDTLLARVQAEEARAKRGRLRIFFGAAAGVGKTYAML
                     EAARSQRSAGTDVVVGYVEPHGRPETERLLEGLERLPTVAVRYRGMIRQELDLDAALR
                     RHPAIALVDELAHSNPIGGEPPLRHPKRWQDVEELLDAGINVWTTVNVQHLESLNDLV
                     AQITGVRQRETLPDKVFDEADDIELIDLPPDDLLARLRAGKVYIPDQVAAAIDRFFRT
                     PNLMALRELALRRVAARVEAAARALPGDRVRTRLAGDRVLVAVGPDRQAEQLVRAGKR
                     LADALDAAWTVVYVETPRLLRLSDADRNRRIDVLRLAESLGAETVTLDGPSPSAALLE
                     YAQTRSATRVLLGTPKRRGWRAWWRPSAATELIRRAKGFDVTIITPAESEDPRARGAA
                     ATVVPARSHPIHWDRYAWAVASSVLCTVIAFGLYPRFELSNLVMVYLLGVTIAGLRLG
                     RGPSVLTSILNVIAFDFFFVPPRFNLAISDVQYLVTFGVMLTVALVTANLMASVRQQT
                     RVAGARERRTALLYAMSRELTTTRGIGNMATVAVRHIAEVFQCQAVILLPDESEHLQY
                     PQDPPVDQSFRSADLAIAQWVADNGRQAGLGTDTLPAASGLYIPLGDPSQKLGVLAVL
                     PSNPRRVLLPEQRHLLEIFTGQTGLALERARLAEAAEAASVAAQRESLRNTLLASISH
                     DLRTPLAAVAGAGSTLATQGASLEEATRTSLARTIEIKAREISDLISNVLDLTRLESG
                     PVTLRRDWQMLDELAGTAYLRFEERLAGHVVDLQLPADLPPVFVDGTLVVQLFANLFD
                     NLAKYTPAGTRARLSAIAEGAFVRLVLEDDGPGFPPGDPARLFEKFQRGSGEGTVVGV
                     GLGLAICRAIVRAHGGQIDASRRAEGGARFEFTLPASQAPP"
     gene            complement(9917..11800)
                     /locus_tag="VFB37_06195"
     CDS             complement(9917..11800)
                     /locus_tag="VFB37_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZO22080.1"
                     /translation="MRSCLKFLLLALAGLGMAATSYAAQTAAIAPGTIVGIVTDTADR
                     PISRATVTAVCLENGSIRATLSGADGVYSFADLPPGTWSVTVHVDGTPDAVARALPVT
                     SGKATRQDIAMNGTPAPPVARQPVLAASPPAGTSAPRGTSPAAPASAVNVPEALQTPE
                     PAPENDTQTPFAVGDLGWMNGYGRATTPIFDTKLFTPEVRFDVNYLQDLNHPKDHTIV
                     GSTEEFRSGEFQIEQVSLGGNFHWHNVQARFLSMFGMFAVTTPRNDASAGVGQWDLQG
                     AYKYFSEANAGYHWDVNHGLNVDAGIFVSYVGLFSYYNFDNWTYQPSFVSSNTPWFFN
                     GLRVQWFPTQTLKIEPWLINGWQSYAKFNKQPGFGGQILWIPNDAFKLVLNSYTLGQD
                     NLASGSGVPNNGGSPTAAIGLANPQAPFINYAHVTRVHEDDSVLWKYFDAKDSAGLSK
                     LAFSLTWDFGCEYGGGVNCTHGPNKANFLGVMLYNRAWFDHDRYAITLGGGFMNNPGR
                     YLALVPPIDGATAQSGTPYFTENPGQALLQWDTQLNFQYMPRDWITWWTEATFRHSNV
                     PYWAGTGGVTPPLGNTGSPASMVCNNGSAIGTDGCANEGGVWYPDLRTREVIWGAGVM
                     VKF"
     gene            complement(11797..12381)
                     /gene="kdpC"
                     /locus_tag="VFB37_06200"
     CDS             complement(11797..12381)
                     /gene="kdpC"
                     /locus_tag="VFB37_06200"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZO22081.1"
                     /translation="MRPALSAMLRPALVLFVIMTLITGVAYPLAITAIAQLLFPRQAN
                     GSLIYREGRALGSQLIGQSFQDPGYFWSRPSATAPQPYNALGSGGSNDGPLSPALLEK
                     VAARIQALRAADPNNRAPIPVDLVTASASGLDPDISLAAANYQVMRVARVRGLPSAAV
                     QRLVDAYAQGRLFGVLGEPRVNVLELNLALESLK"
     gene            complement(12391..14526)
                     /gene="kdpB"
                     /locus_tag="VFB37_06205"
     CDS             complement(12391..14526)
                     /gene="kdpB"
                     /locus_tag="VFB37_06205"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZO22082.1"
                     /translation="MAEQTVLSTSAAPNRAADGMPRPLGRHRLSMFDRALVVPAIADS
                     LRKLDPRTQWRNPVMFVVYVGSLLTTALWIQAIVGRGEAPSWFILNIAVWLWFTVLFA
                     NFAEALAEGRSKAQAASLRGLKQTVLAKRLAETTDRSRQTRVRADLLRKGDVVLVEAG
                     DYIPGDGEVLEGVASVDESAITGESAPVIRESGGDFSSVTGGTRVLSDWIIVRITANP
                     GETFIDRMISMVESAKRQKTPNEIALTILLVALTLVFLFATATLLPYSLYSVAVAKLG
                     SPVTITALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVVAVSGRAVEAAGDVDVLL
                     LDKTGTITLGNRQAARFIPVAGVTEEELADAAQLASLADETPEGRSIVVLAKQRFRLR
                     ERDLRTLGASFVAFSAHTRMSGVDIGERQIRKGAADAIRKQVEARGQVFPRVMTSAVQ
                     EVARRGSTPLLVSEGARVLGLIELKDVVKGGIKQRFGELRRMGIKTIMITGDNALTAA
                     AIAAEAGVDDFLAEATPEAKLRLIRENQAQGRLVAMTGDGTNDAPALAQADVAVAMNT
                     GTQAAKEAGNMVDLDSNPTKLIEVVETGKQMLMTRGSLTTFSIANDVAKYFAIIPAAF
                     ASTYPQLDALNVMRLASPFSAILSALIFNAVIIVCLIPLALKGVKYRPLGAVVLLRRN
                     LLIYGLGGILAPFAGIKLIDLGLVLAHLT"
     gene            complement(14519..16312)
                     /gene="kdpA"
                     /locus_tag="VFB37_06210"
     CDS             complement(14519..16312)
                     /gene="kdpA"
                     /locus_tag="VFB37_06210"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZO22083.1"
                     /translation="MTAASLALITAFLVIALLCVKPLGLYIAHVMEGRPTWASRAGAP
                     LERLIYRVCGINQATEMDWQSYAVALLLFNTVGAGVVYLLQRLQQFLPLNPQQLPGVS
                     PDSAFNTAVSYITNTNWQGYAGETTMSYLTQMAGLAVQNFLSAASGIVVAIALIRGLA
                     RHSTRTIGNFWVDLTRSALYVLLPLSALLAIVLVSQGVLQNFSGYRDVALLESFDSPQ
                     AHSGGAPLEQTVRQHSALRQTLPMGPVASQEAIKELGTNGGGFLNANSAHPFENPTAL
                     SNFLEILAIIIIPAALTYTFGRVVGDTRQGWALLAAMTLLFLSLLWVATRSEQLGNPR
                     LAALGVDQAATALQSGGNMEGKETRFGIGASALFATLTTGTSCGAVNSMHDSFTPLGG
                     LVTLFSMQLGEVVFGGVGTGLYSMLVFALIGVFIAGLMIGRTPEYLGKKIEAFEMKMS
                     ATAILVMPFIALVGAATAVSLEAGRSAVGNPAAHGFAEILYAFTSASNNNGSAFQGLS
                     ANTPFYNISLGVVMWLGRFWPMAAVLAIAGALAAKKRVPITPGTLPTHGPTFIIVLIG
                     TILLVGALTFVPALALGPIVEQLELWTSAHG"
     gene            complement(16312..16401)
                     /gene="kdpF"
                     /locus_tag="VFB37_06215"
     CDS             complement(16312..16401)
                     /gene="kdpF"
                     /locus_tag="VFB37_06215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HZO22084.1"
                     /translation="MSAAHIVSGILALGLLLYLLYALFWPEKF"
     gene            16649..17362
                     /locus_tag="VFB37_06220"
     CDS             16649..17362
                     /locus_tag="VFB37_06220"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZO22085.1"
                     /translation="MLARQALAIRHVAFEDCGTLEAVLARRGFALRYVEAPYEDLPSL
                     DAGSPDLLIALGGPLSVYAEAQYPWILEELHLLERRLASGKPALGICLGAQMLARVLG
                     ARVFPAPVKELGWKPLQLTAAGRSSSVAPLAPEHTSMLHWHGDTFDLPTGATLLASTA
                     EIPQQVYEWGGKVLAFQCHPEICAQHIESWLVGHACEVAATPGVSVGELRERTQQLAP
                     TLAQQAGEAFESWLNSVGL"
     gene            17384..17971
                     /locus_tag="VFB37_06225"
     CDS             17384..17971
                     /locus_tag="VFB37_06225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO22086.1"
                     /translation="MKIRDSGMPEQFVWDGFFDAPRILQKLRLEFSEEDVVDIGCGFG
                     TFSVAAARMTRGIVHALDIDSGMISATLSRARAAGLGNIKAVVRDVVATGTGVPDVAA
                     GYVMLFNILHAEDSLGLLREAHRILRPGGLAALIHWVHEARTPRGPPLEIRPRPQQCQ
                     EWGRETGFVSHETVLLPPHHFGLVLEKPAVRQGAE"
     gene            18023..18421
                     /locus_tag="VFB37_06230"
     CDS             18023..18421
                     /locus_tag="VFB37_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZO22087.1"
                     /translation="MIKVRLSKAETRILEQFWKLGTRSVREILESLPEEEQVAYTTVQ
                     TLVYRLEEKGAVRKVKKIGNAQLFEPAIDQSEFRSGLVRDLLELFGGSPRLLVSNLLE
                     SGTLTLKDLKALQGGATSRTSRPQRGADRR"
     gene            18551..19213
                     /locus_tag="VFB37_06235"
     CDS             18551..19213
                     /locus_tag="VFB37_06235"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO22088.1"
                     /translation="MTLSINDTAPDFEAQTTEGKIRFHDWIGNHWAVLFSHPKDFTPV
                     CTTELGYMAKIKPEFDKRGVKIIGLSVDPLDRHTGWASDIKETQGFAPNYPMIADVDF
                     NVSKLYGMLPAPISGDPTKRTAADNQTVRNVFVIGPDKKIKLVLVYPMSTGRNFDEVL
                     RVIDSLQLTAKHKVSTPVNWKSGEDVIISGSVSDDEAKKQYPQGWKAPKPYIRIVPQP
                     RG"
     gene            complement(19302..19694)
                     /locus_tag="VFB37_06240"
     CDS             complement(19302..19694)
                     /locus_tag="VFB37_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZO22089.1"
                     /translation="MAKRFHPLFRPLSAAQEARARRPALAGQSPAIEAIWQVVQRVPR
                     GRVATYGEVARAAGLPGRARLTGRALRKVPPDMHLPWHRILGAGGRIVFARQSPHHRE
                     QARLLRSEGVPVKDGRVPRSAIMTAEEL"
     gene            19825..20967
                     /locus_tag="VFB37_06245"
     CDS             19825..20967
                     /locus_tag="VFB37_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22090.1"
                     /translation="MTTSARNRIALLACLTVTIAVPCAAAPNMPPAAALPADRNRDGQ
                     HDFDFEVGTWEIHLQRRLHPLTGSNEWVRFDGTSFTRKVWGGRADLEEFETDGAGGRI
                     EGLTLRLYNPQTHQWSLYWANSRDGQIVPAQVGQFSGDRGEFYGYDTLNGRPILIRFI
                     WSKTHTDSPHFEQSFSEDGGRTWEVNWITDQKRVNASAINPHPKPPAVAAGTDKTVPS
                     PAVSRDGQRDFDPLIGDWIFHLKRRLRPLTGSDTWVDLKGTGVCVKVWDGRADLDQVQ
                     LEGPSGHIEGLTLRLYDPQSHQWRLYWANSKDGILVPPQIGEFKDGQGAFYAPDTLNG
                     NAILVRFAWSNLTSASPHFEQAFSSDGGRTWEVNWVTDQTRIGAPH"
     gene            complement(20982..22136)
                     /locus_tag="VFB37_06250"
     CDS             complement(20982..22136)
                     /locus_tag="VFB37_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22091.1"
                     /translation="MRVTAIAITFALGSGLAASDGVDGARGPPHYVVDAGWPEELPNH
                     WIMGQVAGLAVDRQDHLWVLQRPRSATVDELGADQQPPRSECCAHTPAVLEFDSAGRV
                     MNAWGGPGFVPDWPHTEHAIWIDGQGDVWIGGSGSGDRQVLKFSPTGQLLLEIGHPSD
                     APVDNQDTSILGQPAGIQVDDTAHEVYIADGYLNNRVVVYDSVSGQFRRGWGAYGIPL
                     SQIEAATPEKPGSAGADHETGYVPGGAADRQFRTPVHCARLSLDGFVYVCDRRNDRIQ
                     VFTREGHFVKEFVVHASTLGNGSVWTIELSHDRGQHYLLVADGEDNVIWILDRNDGQV
                     VSSLGHNGRNAGQFHWVHQTAMDSRGNLYTGEVDTGKRVQKFVLQGAAKR"
BASE COUNT         4187 a         7277 c         7154 g         3992 t
ORIGIN      
        1 ccgatcgctg agcagggggg cgaagcggcg cggtaaaaac cgaccgcgag aataacgcca
       61 tcgcaaacaa tggcgcgaac tcgctagacc tgggagctcc agcgccgcag cttccacagg
      121 atgcggctca attcgaccgg ctgcacgggc ttcgtgagac agccgatgaa gcctgcagcg
      181 cgggtgagct ccgtgtggcc cagctcctcg cgctctgcga tcgccagcag gggtgttcgc
      241 cggaccccac tgttgggatg cgctcgcagc ttgcgcgcga gctcaaagcc gctcatgtcc
      301 ggcaactcga ggtccaggag cgccaatgag ggagcgaagt ctactgcagc ctggagcgcc
      361 ttgcgcgccg agcaggcggt gagcgtggtg aagcaaccca gttcctgcag cgcatacttg
      421 atcgaatcag ccgagcggaa gtcttcattg acgatcagga ttctgaggcg tgccggggcg
      481 agcgcccgat ccggaacatc ctcgtaggcg ctgaagggaa gacgctggga agtggccagg
      541 gagtgtgttt gcaccgctac ccgctagccg acgcgcgtcg tgaagagcgc agttgctgct
      601 cgtttcgcac atcttccaat gtaaggtgag tcgccgccgc gatcgcgtgc agtgctggat
      661 ggaatgggtc ggatggcatg cgaagctgat gcaccttctc ccacgcgcgg atacgcacgt
      721 cggcggaagt gtccggcgca tgttgttccg ccaggttcac gcgcttcagc tcggcacgct
      781 ccaatgcctc gattacccgg cgctcgcgat aatcaggaag cacgccatcg ctcgcggacc
      841 ctgatcgttc gcttctcgcc tcaggcacga ccgtgatcgc attcattcca gtcccctcgg
      901 tgatgaaggc ccctcgcgcg tgggaggagg gaaagttgcc gacatctgac tgggtcatga
      961 gaatctcgca aggaacctgt aggcggacac gcaaagcgcc gcgggcgcgt cgggagactt
     1021 ttgagatggc actctgacct gcgcgaatgc gccggagtgg cgcaaggcca aggcgaaagg
     1081 atcacgagca ccggcaggat agcacgttcc acgccaaccg gggcgatgaa atcagggggc
     1141 tgaacccttc gttacaggac cctgatcgag gagctgcgcg cctaagacga tcctttcaaa
     1201 gcgtcctcac gccagcttct cgcgactgct ctgcatgggg aaactcacgc gaaaggtcgt
     1261 ccctcgcccc ggagccgtct ccagctccag gccggcatcg agaagctcgc agaggcgctt
     1321 gacgatcgca agaccgatgc cctgcccggg gcgtgagccc gtactcgcgt gttgatcctc
     1381 cgagcgcaga gtgggggctg ctgcgggatc tgccgagcgg tcaccggcgt gcgcggcttc
     1441 cagagcactg tcatgcgcct gctcggtcgc ctgatagaga gcacgcccga ggggaccgac
     1501 acgttccgga tcaaagccgg gtccggtgtc ctgaaccatc agggtccacc gcgaggcacc
     1561 tgcgtccggc gcctgccacg cgagccttac gccgccatgt tcagtagctt tgagcgcgtt
     1621 gagcaccaga ttctggacga tccgctgcag cttcacgtga tcggattcca cgaccaacga
     1681 tgcagggccc tccaacttga ggaacaggtt gcgctcggag gctactgcgc gcactgactc
     1741 gcccaactcc cgaagcacct gagctgcatc gaaccgcgtg atctgcaggg gatcttgccc
     1801 tgcctccagc cgcgcgagat cgatcagatc gccgagcatg aattgcatcg aatgaacggc
     1861 gcgctcgagc accttctcgg accccggcgc agcttcgccg gatcgcgagc gcacgagaac
     1921 ggcggaagcc gtcgcaatca ctccgacgct cccacggatg tcgtgagctg cctcgcgcaa
     1981 gatctcgcct cggctgcgct ccagcgtccg caagtgcgcc agcgcagcct cgaggtcacg
     2041 catgcgactg gccgcctcgg cttgctgcag ccgcgtgtag cgcgccgcgc tttcgctggc
     2101 accctcgccg cacagaccca cgagggcatc tctcgcctcc gcgacgattt ccgtgtccag
     2161 cgccggatta gcacgggcaa actcctcgag ttcccgcaga atgcagcggt gcaaatgcgc
     2221 ccactcgagt atggtttcgc gcagatcgta tccctgctgc cagcgatgca gaccatgctc
     2281 tgcggcaccc ttgcgctgtt ccgcgttcgc ctgcgtgagc cctacgccat catccgccct
     2341 cagtgttttc tcgaatgcct cgagaacgtg cggaatgctg tccatgaatt gcgcgtgggt
     2401 gatcgacgaa gcggtcgtca actgcggatc tttcgccacg cgcgatcgcc actcctcgag
     2461 aattcggttt cgtgctcgtc gaatgtgtcc cgcgagggag acgctctggt tctgggtccg
     2521 tttcacggcc tctccaaata atgtttgcca agccgggagc acgagggcct cgaccgagcc
     2581 cgacacggcg tcatctgcgc cgcgcagggg cccgaccata cccagtccat tgcgggatgt
     2641 cacgaacact cggagcagcg tgggcttagc ttcacgggtc agacgcctcg acggcggtcg
     2701 agggcttcgg ccgggaccac cttcttcagg cgcacacgaa ctcgagcccc cgcgggaaat
     2761 ggcccgctct gcagtacgat aggggtcctg acgcgaggag atctgcatgc atcgctcccg
     2821 cctttgcgcc attttgatcg actgcaagac tactgaggtg gacgaggccg cccgcttctg
     2881 gggtaaggca cttggacggc cgttcgatcc aacgcacccg agcagtcgcg gaaattatcg
     2941 aatgctccag accccgccgg acgagcctat cgtggaaatc cagaaggtcg agcatgacag
     3001 tcgggtccat atcgacatcg agagcgacga cattcccgcc gaggtggcgc gcctgtcgga
     3061 actcggggcg aaggtcgtga cccgactcga gcgttgggtg gtaatggaag ccccgacggg
     3121 tcagcgcttc tgcgtcgtgc gggtgcagcg tcccggcttt ccgaaaaacg ccaacgtgtg
     3181 gccgtgagcc tgccctagcg catgagcgtg atcctgccct agcgcatgag cgtgagcccg
     3241 cccctaacgg cagcggatct tcttgccttc caggaacggt gccaggggta gccgttcgat
     3301 caccacatcc ttgcccgtca ctacgttgtg gctgcgggtc gtcgtggcca tgaacatcga
     3361 attgtaaatg gcctcctccg tcgcttccgc cactgcttca aacaaggggg agatggcatc
     3421 gttggacaat tcggagaatt gaccgatatt cgcgcgcttc tcggaggttc gaatgcctcc
     3481 ttgcgcggtc gagaaggcga tgaagtagtc ccccgagcca ttgctgaaac tcgagcccgt
     3541 gcgggcaacg ccggccgtgg cgcgccgggc cagccgtctc aggttgcgat ccgagagcgg
     3601 cgcgtccgtc gcaatgacga ccatgatgga gccatcagca ctgtggccgg ccagctcgtc
     3661 cttcagatag aacttgccaa gctcctggcc gatccgccgg ccatcgaccg taagcatgcc
     3721 gccaaagttg gactggatga gcacgcccac cgtccagccg ccggagatct cgggcagccg
     3781 ccgggagctg gtgccaatgc cgcccttcca gccgaatgcc acggtgccgg cccccgcgcc
     3841 gaccgctcct tcttcgaccg gacctgcatg cgcggaacgg attgcctgaa gaatctgctc
     3901 ggagctcaat gcgcgtccac ggatatcgtt gagagtgccg tcgttggtct cgcccacgac
     3961 cgcgttggcc gacaagactt tttcgttacc cggctgcgaa agcgtccagt cgagtacggc
     4021 ctccacgccg cgggggactg cgagtgtatt cgtcagcacg accggagctt cgatctctcc
     4081 caactcctcc acctggctga gtccggcaga ttttccgaat ccattcatca caaacacgcc
     4141 tgcgggaacc ttgttctgat acaggtttcc gccgtgaggg agtatcgccg tcgcgcccgt
     4201 tcgtatgctg tcgccttcga ccactgtgct gtgacctaca agcacgccct gaacatcggt
     4261 aatggcgttc agcggcccgg gcgccagcac ccccggcgca atacccaact gacgggcgcg
     4321 cacccgatcg gtctccttga tgcaccccgc tgccgggctc gcggcggcct gagagtcgat
     4381 cgtaaccgcg cacagcagga tcaccagagc cgcgaccagg cgtgaatgga gcacgggagt
     4441 ctccctcgat ggttcgaaac gcccatcatc ggcgccgtgg cccgcagagc acaaggatgc
     4501 aatgggttgc accgcaaggc ctccgagtcg cgccggcagt gagcgctaaa ggcggcgcct
     4561 gactcccctg aggggcgcag gcgccgcgtg tcttggggct agttccttgc cggatccggg
     4621 tacgcgaacg gatcggtcag cttcggctcg gtcggcacgg gcttacgcgg cagcggcttg
     4681 tcggcatcgg cggcttcgag cagcacggaa gccagcacca ccgcgccttc cttcatatca
     4741 tcgatgcgca gatgatcgta cgtgtcgaga tcggtgtgat ggacccgcga ttcgtaatcc
     4801 agtggatcct ggatgaactg aaaggcaggc aggcccagtc ggctcagaaa gacgtgatcc
     4861 gtcgagccgg tcggcctcgc gaccactgaa tcggcactca tgccggcaaa gggtgcaagc
     4921 cactcgcgga aaatcggcac gacgttgaga ttgccctccg tgtagatgcc gcggatcttg
     4981 cccgatccat tgtcgatgtt gaaataggca tccagctcgt tgaagccggg cagcgtccgc
     5041 acaggatagg tctcgctcgc gaaatagggc ggcaggtccc gcagagcggg atcggtcggt
     5101 gcgggccgca cggccagaaa tttctccaca taggccgccg agcccagcag tccctgctcc
     5161 tccccggccc acagcgcaaa gcggatgctg cgcttgggct ttgcgccgac cttcacgaga
     5221 atgcgcgcgg cctccatcac catgatgctg ccggccccgt tgtcggcggc gccatcaccg
     5281 gccacccagc tgtcgaggtg cgcccccgcc atgacgtagc cggatccggc agcgccgccg
     5341 gaaatttccg cgaggatgtt gttggcattg tgatcgccgt cctcatagtg gacgcgattc
     5401 tcgatttcca ccttgacctc acccaccttg gcgagccgcg ccagtttccg atagtcctcg
     5461 gcggccagtt cgacggccgg aagcttcggg ctctggccta ctcgatacat gtaaccttcg
     5521 ccgtgtacga gccggccttc tgatcgcgac atggtgatga gagccagggc accctcctcg
     5581 gccaggaatg catcgcgctg cgcgaagaat ctgaagtgct cgatgcgctt cttgcgctgc
     5641 tcggggtcga acaccggctc ctcgaacttg tccagcttgg cgatgtcggc ctcggagtcg
     5701 cgccgaaatg gaggttccga gtcatctttc tgtggcgcag gccaggtcgc cagaacgatc
     5761 ttgccggcca gcttcccctt ccagtcggca aagtctctct cgctgtcgag cggggccacg
     5821 atgatgggag cggtcaaggt cccgttggtc ggcggtgtcc aggcgactgc aatgcctttc
     5881 atctccacgg gccgcggcga gaccatgcgg atgtgtgagg cctcgatcca ccagccgcgg
     5941 ccgaaatcga acgcctcggt gcgcacattc ttcaggcccc actcgctgaa tttcccggac
     6001 gtccaccgct ccgcgcggcg catcgccgga gagttggtca tgcgaccgcc aatctggtcg
     6061 ctcaggtatt cggctgtctc ggggatcgcg ctgtgattga agccctcatc agcgatgctg
     6121 ttcaggacgg tccgctcggc ggagggtgag cggctgtctg cagcgacggc gtgtacgccc
     6181 aaagacccga cagcaaggct cgcggcccag acgggcaatt tgatcaacag cctgttggtt
     6241 ctcatgtgca tcggggcttc cttcaactga tacagcggcc gccgcgcctg ccgctgcgag
     6301 gctcagcggg gcgccgattg ttccatattc tgccgcgtcg cgcgccgagt tatcgatcag
     6361 ggtccgcaga gtctcgagca aagcggcctg ggcatgctgg gcgctgctac gcagacttcc
     6421 tgatatcgca aggaatctaa tgggtgctca agatatcccg agagggagtc gatcgtcggc
     6481 tatcgggact cctcgatgca gagccggtag ccgatgccaa tttcggtgag gatgtagcgc
     6541 ggccgctgcg gcacgggctc cagtttgtcg cgcaggttct tgatacagac tcgcagcccg
     6601 cgcgcatcgc tcacgcgatc cggcccccag acctgacgca gcagctgctg ctggcgcacc
     6661 acgataccgg tgtggcgggc aagacattcg agcagccgat actccagcgt ggtcagatga
     6721 atttcgccct ggggtccgta cgcacgtcgg cccggcaagt cgatccgtac agtgccgaac
     6781 tgcaggacgg agctctgttc gctcccccgg gcattgcggc gcagaccggc tctcactcgc
     6841 gcgagcaact cggcagcgga gaacggcttg gttacgtagt cgtcggctcc ggcatcaagt
     6901 gatgcaatct tctgctcctc caatgttctc gcggacagca caatgatggg tagcgacgac
     6961 caggctcgca cgtggcgcac gaccgacagc ccgtcattgt ccggcaaccc gaggtccagc
     7021 aaaagcagat ccggcttgtg actgcgcgct tcgatctcgc ctcgctcggc cgtctccgcc
     7081 tcgatgactc cgtacccctc ttgggtgagc agcgtatgca gcacctcacg aatcgcccga
     7141 tcatcttcga cgatgaggat ctgatgggag gtctgcgtca tggaggcgcc tggcttgcag
     7201 gcagagtgaa ctcgaagcgt gcgccgccct ctgctcgccg gctcgcgtcg atctggccac
     7261 cgtgcgcgcg cacgattgcc cggcagattg ccagcccgag gccgacgccg accacggttc
     7321 cctccccgct gccgcgctga aacttctcga acagacgagc cggatcaccg ggtggaaatc
     7381 ccgggccatc atcctctaac acgaggcgta cgaacgcacc ctcggcaatg gccgaaagcc
     7441 ttgctcgggt acccgccggt gtgtatttgg cgaggttgtc gaacaggttc gcgaagagtt
     7501 gcacgaccag cgtcccgtcc acaaagaccg gcggcagatc agcgggaagc tgaagatcga
     7561 ccacgtggcc ggcgagccgc tcctcgaacc gcagataggc tgttccggcg agctcatcca
     7621 gcatctgcca atcgcgacgc agagtgaccg gtcccgattc caggcgcgtc agatccagca
     7681 cgttcgagat gagatctgag atctcacgtg ccttgatttc tatggtcctg gccagtgaag
     7741 tccgtgttgc ctcctcgagc gaggcgcctt gcgtggccag cgtactcccg gcgcctgcaa
     7801 cggccgcgag cggtgttcga agatcgtgcg aaatcgaggc caggagcgta ttgcgcagac
     7861 tctcacgttg ggccgccacg ctcgctgctt ccgctgcttc cgccagacgg gcacgctcca
     7921 gggcaaggcc ggtctgaccc gtgaaaatct cgagcaggtg ccgttgctca ggtaacagaa
     7981 cgcggcgtgg gttggagggc agtacagcta gcacaccgag cttctgggac ggatcgccga
     8041 gcgggatgta gagtccggag gctgccggca aggtgtcggt gccgagaccg gcctggcggc
     8101 cgttatcggc cacccattgc gcgatggcga gatccgcgga tcggaaggac tgatcgaccg
     8161 gcgggtcctg cggatattgc aggtgctcgc tctcatccgg cagcaggatg actgcctggc
     8221 attgaaacac ctcggcgata tggcgcacgg cgaccgtagc catgttgccg atgccgcgcg
     8281 tggtggtcag ctcgcggctc atggcataga gcagcgccgt gcgccgctcg cgcgcccccg
     8341 cgacccgggt ctgctggcgc acactggcca tgagattggc cgtgacgagc gctactgtga
     8401 gcatgacccc gaaggtgacg agatactgga catcggaaat cgccaggttg aatcgcggcg
     8461 gcacgaagaa aaagtcgaag gcgattacgt tcaaaatgga agtcagcacg gaaggaccgc
     8521 gcccgagtct gagccccgca atggtgacac ccagcaggta gaccatcacg aggttcgata
     8581 gctcgaatcg aggatacagg ccgaatgcaa tcacggtgca tagcacgctg gatgcgacgg
     8641 cccaggcata tcgatcccag tggatcgggt gcgaacgcgc cggaacaact gtggccgcgg
     8701 cacccctcgc acgcgggtct tcactttcgg caggagtgat gatggtgaca tcgaaccctt
     8761 tggctcttcg tatcagctcg gtcgcggcag acgggcgcca ccacgcccgc cagccgcggc
     8821 gcttcggcgt gcccagcagc acgcgcgtcg cgctgcgagt ttgcgcatac tcgagcaagg
     8881 ctgccgaggg tgatggaccg tcgagggtca cggtttcggc accgagcgac tccgccaagc
     8941 gcagcacgtc gatgcggcga tttcggtcgg catcggaaag ccgaagcagt ctgggcgttt
     9001 cgacgtagac gaccgtccag gcagcatcca aggcatcggc caggcgcttg ccagcacgca
     9061 cgagctgctc tgcctggcga tccggcccga ccgcgacgag aacacgatct ccggcaagtc
     9121 gcgtgcgcac gcgatcgcca ggcaacgcgc gcgcagcagc ctcgacacga gcggcaaccc
     9181 gccgcaaggc cagttcgcgc agggccatca gattgggcgt gcggaagaag cggtcgatgg
     9241 ctgccgcaac ctggtcgggg atgtacacct ttccggcacg taggcgcgcc aacagatcat
     9301 ccggcggcag atcgatcagc tcgatgtcat ccgcctcatc gaacactttg tccggcagcg
     9361 tctcacgctg gcggaccccg gtaatctgcg ccacgagatc gttgaggctc tccagatgct
     9421 ggacgttgac cgtcgtccag acattgatgc ccgcatcgag cagctcttcg acgtcctgcc
     9481 accgcttggg atgtcgcagg ggcggctcac cgcctatggg gttggaatgg gccagctcgt
     9541 ccaccagcgc gatggccgga tgccgcctca gcgccgcatc gagatcgagc tcctggcgaa
     9601 tcattccccg gtagcgaacg gccacggtgg ggagccgttc gaggccttcc agcagccgtt
     9661 cggtctcggg gcgaccgtgc ggctcgacgt atccgaccac gacatccgta ccggcggatc
     9721 gctgactgcg ggccgcctcc agcatggcgt acgtcttgcc gacccctgcc gcggctccga
     9781 aaaagatgcg caatcggccg cgcttcgcgc gcgcctcctc ggcctggacg cgagccagca
     9841 gcgtgtcggg atccggccgg ggatcagcca tggaggcctt catgagcgcg caggacaggc
     9901 cgcaatgtta ctccgctcag aacttcacca tgaccccggc cccccagatg acctcgcgcg
     9961 tgcgcagatc gggataccag acgcccccct cattcgcgca accatccgta ccgatcgccg
    10021 aaccgttgtt gcacaccatg ctggcggggg agcccgtatt gccgagcggc ggggtcacac
    10081 cccccgtgcc ggcccagtag ggaacgttgg aatgccggaa cgtggcctcc gtccaccagg
    10141 tgatccagtc ccgcggcatg tattgaaagt tgagctgcgt atcccactga aggagcgcct
    10201 gtcccgggtt ctccgtgaag tatggggttc cgctctgcgc ggtcgcaccg tcgatgggcg
    10261 gcacgagcgc cagataacgc ccgggattgt tcatgaagcc gccacctaga gtgattgcgt
    10321 accggtcgtg atcaaaccag gcgcggttgt agagcattac acccaggaag ttcgccttgt
    10381 tcggcccatg agtacagttg acgcccccgc catactcaca gccgaagtcc caggtcaatg
    10441 agaacgcgag cttggagagc cccgcactgt ccttcgcatc gaaatacttc cacagcacgc
    10501 tgtcgtcttc atgaacccgg gtcacgtgtg cgtagttgat gaatggggcc tgcggattgg
    10561 ccagaccgat cgccgcggta gggctgccac cattgttggg cacgccgctg ccgctcgcca
    10621 gattatcctg gccgagtgtg tagctgttca atacgagctt gaatgcatcg ttgggtatcc
    10681 ataggatctg accgccgaag cccggctgct tgttgaactt ggcgtacgat tgccagccat
    10741 tgatcagcca cggctcgatt ttgagtgtct gcgtcggaaa ccactgtaca cgcaggccgt
    10801 tgaagaacca cggtgtgttg gatgagacga aggaaggctg gtaggtccag ttgtcgaagt
    10861 tatagtagct gaagagtccc acgtaggaga cgaagatgcc ggcatccaca ttcaacccat
    10921 ggttgacatc ccagtggtat ccggcgttgg cttccgaaaa atacttatag gcgccctgca
    10981 gatcccactg gcctacgccg gcgctcgcat cgttacgcgg cgtcgtcact gcaaacattc
    11041 cgaacatgga caggaagcgc gcctggacgt tgtgccaatg gaagttgccg cccaggctga
    11101 cctgctcgat ctggaactcc cccgagcgga actcctccgt cgagcccact atggtgtgat
    11161 ccttggggtg gttgaggtcc tggaggtaat tgacatcgaa gcgcacctcg ggcgtgaaga
    11221 gcttggtgtc gaagatcgga gtcgtcgctc tcccgtagcc gttcatccag ccgagatcac
    11281 ccaccgcgaa gggggtttgg gtatcgttct ccggcgcagg ttcgggagtc tgcagcgctt
    11341 cgggcacgtt gacggcgctg gcgggggccg ctggcgatgt gcctcggggg gcggacgtcc
    11401 ctgcaggcgg agatgccgca agcactggtt gccgcgccac ggggggtgca ggcgtcccat
    11461 tcatggcgat gtcctggcgc gtggccttgc cagaagtcac gggcagagca cgcgcgactg
    11521 catccggagt tccgtccacg tgcacggtta cggaccacgt ccccggtggt agatccgcga
    11581 acgagtacac cccatccgct cccgaaagcg tggcgcggat gctgccgttc tcgaggcaga
    11641 cggctgtcac ggttgcgcgg ctgattggtc ggtcagcggt atcggtgacg attcccacaa
    11701 tcgtccccgg cgcaatggct gctgtctgag cggcgtacga ggtcgcggcc atcccaagtc
    11761 cggcgagagc gagcagcagg aacttcagac agctcctcat ttcaagctct ctagggccaa
    11821 attcaactcg agcacgttca ctcgcggctc tccaagcaca ccgaacaggc gcccctgcgc
    11881 gtatgcatcc acgaggcgct gcaccgccgc ggacggcagg cctcgaacgc gcgccacacg
    11941 catgacctga tagttcgccg cggcgagact gatgtcagga tccagcccgc tggcggaggc
    12001 ggtcacgaga tcgaccggaa tgggcgcccg attgttggga tcagcggcgc gcagtgcctg
    12061 aatgcgcgcc gcgaccttct ccagcaatgc gggactcagc ggcccgtcgt tcgaaccacc
    12121 ggagccgagc gcgttgtacg gttgcggcgc agttgccgag ggtcggctcc agaagtaccc
    12181 gggatcctgg aagctctggc cgatcaactg cgagcccagc gcacggccct cccgatagat
    12241 gaggctgcca tttgcctgtc gcgggaagag cagctgtgca atggcggtga tcgccagcgg
    12301 ataggcgacg cccgtgatca gtgtcatgat cacaaacaac accagagccg ggcggagcat
    12361 cgcgctcagg gcaggacgca tgtcgcttcc tcaggtcaga tgggcaagca ccaggccgag
    12421 gtcgatcagc ttgatgccgg caaagggggc gaggatccct cccaacccgt agatcaacag
    12481 attgcggcgc agaagcacga cggcccccag cggccggtac ttcacgccct tcagggcgag
    12541 cgggatcagg cagacgatga tcacggcgtt gaagatcagc gccgacagga ttgccgagaa
    12601 cgggctggcg aggcgcatca cattgagcgc atcgagctgc gggtaagtcg aggcaaaggc
    12661 agccggtatg atcgcgaaat acttggcgac atcgtttgcg atgctgaagg tggtcagtga
    12721 gccgcgcgtc atcagcatct gcttgcccgt ctccaccacc tcgatgagct tggtagggtt
    12781 ggagtcgaga tccaccatgt tgccggcctc tttcgcagcc tgcgtgccgg tattcatggc
    12841 gacggccacg tctgcctgcg cgagcgccgg cgcatcgttc gtgccgtccc cggtcatggc
    12901 gaccaggcgc ccctgggcct ggttctcgcg gatgagacgc agcttcgcct ccggagtcgc
    12961 ctcggccagg aaatcatcca caccggcttc ggcggcgatg gccgccgcgg tgagcgcgtt
    13021 atcccccgtg atcatgatgg tcttgatgcc catccggcgc agctcgccga agcgctgctt
    13081 gatgccaccc ttgaccacgt ccttgagctc gatcaggccg agaacgcgag ccccctcact
    13141 caccaggagc ggggtgctgc cgcgacgcgc aacttcctgt acagccgaag tcatcaccct
    13201 ggggaagacc tggccgcgcg cttcgacctg cttgcggatg gcatcggcgg cgcctttgcg
    13261 gatctggcgc tcgccgatat ctacgccact catgcgcgtg tgggcggaga aggcgacaaa
    13321 gctggccccg agagtccgta aatcacgctc gcgcagcctg aaacgctgct tcgccagaac
    13381 gacgatgctg cgtccctcgg gcgtctcgtc cgcgagcgag gcgagctgcg cggcatccgc
    13441 aagctcctct tccgtgacgc ccgcaaccgg aatgaacctt gccgcctgac ggttgccgag
    13501 agtgatcgtg ccggtcttgt ccagcagcag cacatcgacg tcacccgccg cttccacggc
    13561 acgtcccgac accgcgacga cgttggcctg catcatgcgg ctcatgcctg ccacaccgat
    13621 ggcagacaga agaccaccga tggtcgtcgg aatgagacag accagcagag ctatgagagc
    13681 tgtgatcgtg acgggtgagc ccagctttgc cacggccacg ctgtacaggg agtacggcag
    13741 caaagtggcc gtggcaaaca ggaagaccag agtcagagcg acgagcagga tggtgagtgc
    13801 gatctcattc ggggtcttct gccgcttggc gctctccacc atgctgatca tgcgatcgat
    13861 gaaggtctcg cccggattgg cggtgatccg cacgatgatc cagtccgaaa gcacgcgggt
    13921 gccgcccgtc accgaggaga aatcgccccc tgactcccgt atgaccggcg ccgactctcc
    13981 ggtgatcgca ctctcatcca ccgatgcaac gccttcgagc acctccccgt ctccggggat
    14041 gtaatcgccg gcctcgacca gcaccacatc ccctttgcgc agcagatccg cgcgcacgcg
    14101 cgtttgacga gagcgatcag tcgtctcggc gagcctcttg gcgagaaccg tctgcttgag
    14161 tccgcgcagg ctggccgcct gtgccttgct gcggccctcg gcgagggctt cggcaaaatt
    14221 cgcgaacagg acggtgaacc acagccagac ggcgatattg aggatgaacc aggaaggggc
    14281 ctcgccgcgg ccgacgatcg cctggatcca cagagccgtc gtgaggaggc ttccaacata
    14341 cacgacgaac atcaccggat tgcgccactg cgtccgcgga tcgagcttac gcagcgagtc
    14401 agcgattgcc ggaaccacca gcgcgcggtc gaacatcgac agcctgtgcc gacccagcgg
    14461 cctgggcatc ccatccgcgg ctcgattcgg ggccgcggaa gttgacagga ccgtttgctc
    14521 agccatgggc gctggtccag agctcgagct gctcgacgat cggtccgagc gccagcgccg
    14581 ggacgaaggt caaagcgccg acgagcagaa tcgtaccgat caatacaatg atgaaggtcg
    14641 gtccatgagt ggggagcgta ccgggcgtga tcggcaccct cttcttcgcg gcgagtgcac
    14701 cggcaatggc cagcaccgcg gccattggcc agaagcgacc gagccacatc accacgccga
    14761 gactgatgtt gtagaaaggc gtgttggccg agaggccctg gaaggcgcta ccgttgttgt
    14821 tgctcgcaga ggtgaacgcg taaaggatct ccgcgaagcc gtgggctgcg gggttcccca
    14881 cagcggaccg gcccgcctcg aggctgacgg ccgtcgctgc tccgaccagc gcgatgaacg
    14941 gcatgacgag gattgccgtg gcgctcatct tcatctcgaa ggcttcgatc ttcttgccca
    15001 gatattccgg tgtgcgtccg atcatgagcc cggcaatgaa gaccccgatc aaggcgaaca
    15061 cgagcatgga gtacaacccg gtccccactc ctccgaagac aacttcgccg agctgcatgc
    15121 tgaacagggt cacgagccct cccagcggtg tgaaggagtc gtgcatggag ttcaccgcgc
    15181 cgcatgaggt gcccgtggtg agggtcgcaa agagcgcgga ggcgccaatg ccgaagcggg
    15241 tctccttgcc ttccatgttc ccgccagatt gcagtgcggt ggcggcctga tcgacaccca
    15301 atgcagcgag ccggggattt cccagttgtt cgctgcgggt ggctacccaa agcagactca
    15361 ggaacaggag ggtcatggcc gccaggaggg cccagccctg cctcgtatcg cccactacgc
    15421 ggccgaaggt ataagtgagc gccgcgggaa tgatgatgat cgcgagtatc tcgagaaaat
    15481 tcgacagagc ggtaggattc tcgaagggat gggccgaatt ggcgttgagg aaacctccgc
    15541 cgttggtgcc gagctccttg atggcctcct gggaagccac aggtcccatc ggtagtgtct
    15601 gtcttagcgc gctgtgctgt cgaacggttt gctcgagcgg cgccccaccg ctgtgagcct
    15661 ggggcgagtc gaaactttcg agaagggcaa cgtcccgata accgctgaag ttctgcagca
    15721 cgccttgcga cacgagcacg atcgccagca acgccgacaa cggcagtaac acgtacagcg
    15781 cggatcgtgt cagatcaacc cagaagttgc cgatggtcct ggtgctgtgg cgggccagac
    15841 cgcggatcag cgcgatggcg acgacaatcc cgctcgccgc cgagaggaaa ttctgcacgg
    15901 cgagcccggc catctgcgtg agatagctca tggtcgtctc gcccgcgtag ccctgccagt
    15961 tcgtgttggt gatgtagctg acggcagtat tgaaggcgga gtcgggggag acccctggca
    16021 gctgttgcgg attcaggggt aggaactgct gcaggcgctg cagcagatac acgacaccgg
    16081 cacccaccgt attgaatagc aacagcgcta ccgcatagct ttgccagtcc atttccgtgg
    16141 cttgattgat cccgcagacc cggtagatca gccgttcgag cggtgcaccg gcgcgcgagg
    16201 cccaggtcgg ccgcccctcc atgacgtgcg cgatgtacag ccccaagggc ttcacgcaca
    16261 gcagcgcgat gaccaggaac gcggtgatca acgccaggga cgcggccgtc atcaaaactt
    16321 ctccggccag aacagggcgt agagcagata gaggaggagt cccagcgcga gaatcccgct
    16381 cacgatgtgg gcagcactca tcccaggtgt gccagggccc ggatgagcca gtgggtggct
    16441 gcgtagagga tcacgataag ggccacagct gctgcatcca ttgcgattct ccagcttgct
    16501 gataactgcg aggcggaggg gcctactgag cggcaacgct ctccgacgca cgagccgtca
    16561 aggctatcga cgcgcgcatt agcaagtcgt taagagattt tccagggcga tctgctatct
    16621 tcggccgatc acttgcgcag gagagacgat gttggcacgg caggcgcttg cgatccgtca
    16681 cgtcgccttc gaggattgtg gaacgctgga agctgtattg gcgcggcggg gattcgccct
    16741 tcgctatgtc gaagccccgt acgaagacct gccgagcctc gatgcgggct cacccgatct
    16801 gctcattgcg ctcgggggcc ccttgagcgt ctacgctgag gcgcagtacc cctggatcct
    16861 cgaggagttg cacctgctgg agcgccggct ggcctcaggc aagcccgcac tggggatctg
    16921 cctcggcgcg caaatgctgg ccagggtgct tggcgcccgc gtctttccgg ctcccgtcaa
    16981 ggagctcggc tggaagcccc tgcagctgac tgccgcaggc agaagctcat cggtcgcgcc
    17041 gctggcgcca gagcacactt cgatgctgca ctggcatggc gacactttcg atctgccgac
    17101 gggcgccacc ctgctcgcct ccaccgccga aattccgcag caggtctacg agtggggtgg
    17161 caaagttctc gccttccagt gccaccccga gatctgcgcg cagcatatcg agtcgtggct
    17221 cgtaggccat gcctgcgaag tggccgcgac tccgggcgtc agcgtcggcg agttacgcga
    17281 gcgcacgcag caactcgcac ccaccctcgc gcaacaggct ggcgaggcgt tcgagagctg
    17341 gctgaactcc gttggattgt aacgactgac tcacgaacgg ccgatgaaga tacgtgacag
    17401 cggcatgccc gagcagttcg tctgggacgg attcttcgat gccccacgga ttctgcagaa
    17461 gctgcggctc gagttctcgg aggaggatgt cgtagacatc ggctgcggct tcggcacgtt
    17521 ctcagtcgcg gccgcgagga tgactcgcgg catcgtccat gcgctggaca ttgattccgg
    17581 catgatcagt gcaacactca gcagagcccg cgcagcgggt cttggcaata tcaaagccgt
    17641 cgtgcgcgat gtcgtggcga ccggcacagg tgtgccggat gttgcggcag gctacgtcat
    17701 gctgttcaac atcctgcatg ccgaagattc cctcggactg cttcgcgagg cgcatcgcat
    17761 cctcaggcct ggcggactcg ctgccctcat ccattgggtg cacgaagcac ggacgcctcg
    17821 agggccacca ctggagatcc gccctcgccc gcagcagtgc caggaatggg gccgagaaac
    17881 gggattcgtg tcccatgaga ctgtgctgct tccgccccat cattttggcc tggtgctcga
    17941 gaagcctgcg gtgcgccagg gtgccgagtg acgtcgcggc ctgctcctgt agtagcatag
    18001 ccgcggcagg taggaggctc gcatgatcaa agtccgtctt tcgaaggccg agacgcgcat
    18061 cctggagcag ttctggaagc tcggcacgcg ctcggtgcgc gaaatcctgg agagcctccc
    18121 cgaggaagag caagtcgcct acaccacggt gcagacgctg gtgtatcgcc tggaagaaaa
    18181 aggcgcagtg cgcaaggtga agaagatcgg caacgcgcag ctgttcgagc ctgccatcga
    18241 tcaaagcgaa tttcgcagcg ggctcgtccg ggacctgctc gagttgttcg gcggttcccc
    18301 gcgcctgctg gtctcgaacc tgctggaaag cggcaccctg acactcaagg acctcaaggc
    18361 gttacagggc ggcgccacaa gccgcacgag ccggccgcag agaggcgcag acaggcgctg
    18421 agctgcgcat tgcgaaacct cgtgaaaaag gcgaggatcg cgccaactca tccggcggac
    18481 gccggtcata agcgctggtt ctgtgaatat tcccggtcgg catcagcacc gggcaagttg
    18541 gaggcttctc atgacactct cgatcaacga tacggcgccc gatttcgagg cccagaccac
    18601 tgagggcaag attcgctttc acgattggat cggcaaccac tgggccgtgc tgttctcgca
    18661 tcccaaggac ttcaccccgg tgtgcacgac cgagctcggc tacatggcca agatcaaacc
    18721 cgagttcgac aagcgcggcg tcaagatcat cggcttatcg gtcgatccgc tggatcgcca
    18781 caccggctgg gccagcgaca tcaaggagac gcagggattt gcgcccaact atcccatgat
    18841 cgccgacgtc gacttcaacg tatccaagct gtacggcatg cttcccgctc cgatttcggg
    18901 tgatccgacc aagcgcaccg cagcagacaa tcaaacggtg cgcaacgtct tcgtcatcgg
    18961 tccggacaag aagatcaagc tcgtgctcgt ctatccgatg agcaccggcc gcaacttcga
    19021 cgaggtgctg cgggtcatcg attcgctgca gctcaccgcc aaacacaagg tctcgacccc
    19081 ggtgaactgg aagagcggcg aggatgtgat catctcgggc tccgtttccg atgacgaggc
    19141 caagaagcag tacccgcagg gatggaaggc accgaagccc tatatccgca tcgtccccca
    19201 gccacgaggc tgaagcggcg caacagtcgt gcagcggcgc ccgcgcaagg cgccgctgct
    19261 gccgggccgc cgcaccccgg cggaagcatc ggtccgctcc gctagagctc ctccgccgtc
    19321 atgatggcgg accggggcac gcggccgtct ttgactggca cgccctcgct gcgtagcagg
    19381 cgcgcctgct cgcgatggtg aggcgactgc cgggcgaaaa cgatgcggcc gccagcgccc
    19441 aggatccgat gccacggcag atgcatgtca ggtggcacct tgcgcagagc gcgccccgtt
    19501 agccgcgcgc gtcccggtag accggccgca cgcgccacct cgccgtaggt tgctactctg
    19561 ccccgcggca ctctctggac gacctgccag attgcctcga tcgccgggct ctggccggca
    19621 agggcgggtc ggcgagcccg cgcctcctgc gccgcgctca acggcctgaa cagcgggtga
    19681 aaccttttgg ccatgccggt gccttgtgtc gagggtccgt cgctctgggg cggcaatttg
    19741 cggcgcgccg tctttgccgt ctatagtagc accacctgcc tctgtagtag atcggcgggg
    19801 atttccggga ggcttggaca ttccatgacc accagtgcca gaaaccgcat tgctctgctc
    19861 gcatgcctga cggtgaccat cgcggtgccc tgcgcagccg cccccaacat gccgcccgct
    19921 gccgcgctac ctgcagatcg caaccgcgat ggccagcacg acttcgactt cgaggtcggc
    19981 acgtgggaga tccatctgca gaggcgattg catccgctca ccggctcgaa cgaatgggtc
    20041 cgcttcgacg ggacctcgtt cacacgcaag gtctggggcg ggcgtgccga tctggaagag
    20101 ttcgagacgg acggcgccgg gggccggatc gagggtctga ctttgcgcct ctataatccg
    20161 cagacgcacc aatggagcct gtactgggcc aacagcaggg atggccagat cgttcctgcc
    20221 caagtgggtc agttcagcgg ggatcgtggc gagttctacg gctatgacac actcaacggc
    20281 cggccgatcc tcatccgctt catctggtcg aagacgcaca cggattcacc gcacttcgag
    20341 cagtcgttct ccgaagatgg cggccggacc tgggaggtga actggatcac cgaccagaaa
    20401 cgggttaacg cgagcgccat caatcctcat ccgaagcccc cggctgtcgc ggcaggcacg
    20461 gataaaacgg tccccagtcc cgcggtgagc cgcgacggcc aacgtgactt cgatcccctg
    20521 atcggcgact ggatcttcca cctgaagcgc cgtctgcgtc ccctcaccgg ttccgatacg
    20581 tgggtcgatc tcaagggcac gggtgtctgt gtcaaggtat gggacggccg cgccgatctg
    20641 gatcaggtgc agctcgaggg cccatcgggg cacatcgagg gactcacgtt acgtctgtac
    20701 gatcctcagt cgcaccaatg gcggctctac tgggccaaca gcaaggatgg catcctggtg
    20761 ccgccccaga tcggggaatt caaggatggt cagggggcgt tctacgcccc ggacacgctc
    20821 aacgggaacg ccattctcgt gcgtttcgcg tggtcgaacc tgacgagcgc atcgcctcac
    20881 ttcgagcagg cgttctccag cgacggtggc cggacctggg aagtcaactg ggtcacggac
    20941 cagacacgca ttggagcacc ccactgagag gcgtggcagg ttcagcgctt cgctgcaccc
    21001 tgcagcacaa acttctgcac gcgtttgccg gtatccactt cgccggtgta cagatttccc
    21061 ctcgagtcca tggccgtctg atgcacccag tgaaactggc cggcattgcg cccgttatgg
    21121 cccaagctcg acaccacctg gccgtcgttg cgatcgagaa tccagatcac gttgtcctcg
    21181 ccgtcggcca ccagcagata gtgctgcccg cgatcgtgtg agagctcgat cgtccagacc
    21241 gagccgttgc ccagtgtcga ggcgtgcacg acgaactcct tcacgaaatg cccctctctg
    21301 gtgaagacct gaatgcgatc gttgcgccgg tcgcacacgt agacgaatcc gtcgagcgac
    21361 aatcgagcac agtgcaccgg agtgcggaac tgccgatctg cagcaccacc gggcacgtag
    21421 cccgtctcgt gatctgcgcc agcgctgccc ggcttctcag gcgtggcagc ttcgatctgg
    21481 ctcaacggga ttccgtaggc gccccagcca cgccggaact gaccggatac cgagtcatag
    21541 accacgacgc ggttgttcag gtacccgtct gcgatgtaca cctcgtgcgc cgtgtcatcc
    21601 acctggatgc ccgccggctg gccgagaatg ctcgtgtcct ggttgtcgac cggcgcatcg
    21661 ctcggatggc cgatctccag taacagttgc cctgtgggcg agaacttcag gacctgacga
    21721 tcaccgctgc cgctgccccc gatccagaca tcgccctgcc catcgatcca gatggcgtgc
    21781 tcggtgtgcg gccagtccgg cacgaacccc ggtcctcccc aggcattcat gacacgaccc
    21841 gcggaatcga attccaggac ggccggagtg tgcgcgcaac attcggagcg cggtggctgc
    21901 tgatcggcgc cgagctcgtc aacggtcgcg ctgcgcggcc gctgcaggac ccagaggtga
    21961 tcctgtcggt ccacggcgag gcccgccacc tgtcccatga tccagtggtt cggcagctcc
    22021 tcgggccagc cggcatcgac gacatagtga ggcgggcccc gtgcaccgtc cacgccatcg
    22081 ctcgccgcca ggcccgaccc gagagcgaat gtgatcgcga ttgcagtgac tcgcacgtct
    22141 gactccaatg tgaaccccat cacggagcgg gtggattgta ggcgatgacg gcagccccgg
    22201 cgcgcgcccc ctggaggccc gtgagctcgc gccggaacgc ttgaattcac ggattgagcg
    22261 cgccggcaga gcgtttcgtc ccattgcagt agggcgcacc ggcgctcgcg cgtatacttg
    22321 cgccaggccg gagcccttgc ttcgacaggt agatctcaca tgctcaggaa acttacgatt
    22381 tgcgttgctg tatgtgcctt ggtgggcgga tgcgccaatg gatggccgcg cgtgcatcat
    22441 ctgcatcaga cagcggacgc cagttgtatc ccgacaggct ccaagattcc ccgatcggat
    22501 tgcgccgtgt cgcaaccgac cagcagcacc tcacagggcg atctggaccg ccaggggggc
    22561 cagggatttg gtaccgtgaa cgtccctgct caggggccag cgggaccgag
//
LOCUS       DASRHN010000161        11680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_383701, whole genome shotgun sequence.
ACCESSION   DASRHN010000161 DASRHN010000000
VERSION     DASRHN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383701"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VFB37_06255"
     CDS             complement(<1..1375)
                     /locus_tag="VFB37_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22092.1"
                     /translation="MTGPTQRAPGAPPLPDTPFRFICYFIARFRGWYLAMVLLEAVNS
                     TCTILIPYATGQIIKAVTSAHEHSMSYVAVLRGPLWLFIALAIGEVIFSRAGGMCQIL
                     IGPRQRQAVTRAMYAYLQHHSHRYLSNDFAGALAHRISETSMGVSQTLWTVIFDFWPM
                     AIVFAVAIVMLAYAHPGLATFVGVWALAFVCASFWLATLTRPFAVAAAAARSETTGKV
                     VDAVTNLTSTRLFARLRYEREYLDGQLRKELRAVRLSSWSSERVRWFQYTAAAILKIG
                     VLYYALRLWGSGQISVAEFVVASTTTLLVINEARNLSRRFLEFFEYVGNVTNGVHTII
                     RPHEIVDRPDARALAITRGRIQFRNVTFAYASERPVFRNLSVEIAAGQRVGLVGFSGS
                     GKSTFVSLILRLYDPQQGAILIDGQDIRAMTQESLHAQLSLIPQDPNLFHRTLLENIR
                     YGRLEATD"
     gene            complement(1404..2195)
                     /gene="modA"
                     /locus_tag="VFB37_06260"
     CDS             complement(1404..2195)
                     /gene="modA"
                     /locus_tag="VFB37_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZO22093.1"
                     /translation="MQSASPLRVLLLWVVATFLTAAAGTVPARAAGTVMVFGAASLTE
                     VMGEMSAAYTQRTKIPIKASYAASSVLARQIVAGAKADLFFSADVQWMDYLDTRQLLR
                     PGSRHEAVGNQLVLIAPADSRVSVRLAPGANLGAALGPQGKLAIGDPDSVPAGIYARA
                     ALRKLGMWNSLYDRIVGAENVRAALAYVARGEAPLGIVYRTDALAEKRVRIVAQFPPN
                     THPPIVYPIALTIGADSAAAEFEDFILHSREAQALFSKYGFEPLH"
     gene            complement(2219..2860)
                     /locus_tag="VFB37_06265"
     CDS             complement(2219..2860)
                     /locus_tag="VFB37_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22094.1"
                     /translation="MSVASRPVTTGKTRTCPHCKATILESLSICPGCMHHLRFDSEAA
                     KRQVAATPALRVEGIIRHPPLDQPLEYFVIIAIRNDRGEEVTRQVVNVGALQAAEKRT
                     FTLSVEVLPPQVTGTSKAGTSAAPPKPSAPQPSATVRAPPAGAPSAAGTPGTSTTSTN
                     PVLPAPASQGSAAPGATPGSPPAGSSTSSTTRSPQTGYPGGLKPPIPGGPKRH"
     gene            complement(2857..3393)
                     /locus_tag="VFB37_06270"
     CDS             complement(2857..3393)
                     /locus_tag="VFB37_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZO22095.1"
                     /translation="MALWKDTNTGPGPMPAQNLPPAAGPLPHPREPEKTNVAPLSPDP
                     ARRPVKESQRLDMKESIIASDLTIEGKIIGSGHVRIAGRFKGDVHVDGNVTLDTGAHL
                     EGQVKASVVVVGGELLGNIDNAKRVELLEGGVISGDVKAGSLTVAAGSRMRGQVEFGW
                     ADGESRSKPETKGYGTGS"
     gene            complement(3776..4162)
                     /locus_tag="VFB37_06275"
     CDS             complement(3776..4162)
                     /locus_tag="VFB37_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22096.1"
                     /translation="MDPGQKPLRVLIAENHRDLSEVMSQLIDTEPDMHCVGQVSDAAS
                     VVPMARQSSANVLILDLTLHGGSGLPLIEELTTCMPDLRVIVFSGLADSENVAREARR
                     RGAAAYVTKGCDLQVLLEALRPNKLA"
     gene            complement(4247..5605)
                     /locus_tag="VFB37_06280"
     CDS             complement(4247..5605)
                     /locus_tag="VFB37_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HZO22097.1"
                     /translation="MRNLNAFKAYDVRGRIPDEINEQLAYDIGRAYCAFVKPGTVAVG
                     HDIRLSSPTLAAALKRGLIESGCSVKDIGLWGTEGAYFATFAEGLDGGIMVTASHNPS
                     DYNGMKFVREQARPISADTGLRQMRALIESGQPPPGAGRKGSEQPLDIRPRYLEHLLS
                     YVDRAKLRALKVVVNAGNGGAGLVIDQIEPHLPFEFVKVHHEPDGAFPNGIPNPMLEE
                     NQAATADVVRSSGADVGLAWDGDYDRCFFFDEQGRFIEGYYLVGLLAEAFLRTEPGAR
                     IVHDPRLTWNTLDIVRSKGGVPVLCKSGHAFIKQKMREVDAAYGGEMSAHHYFRRFSY
                     CDSGMIPWLLVLQEIGQRGQTLSELLGERMRLFPASGEINRRLAGEPQAILARVRQRY
                     EKGALAIDLTDGLSIEYRDWRFNLRASNTEPLVRLNVESRGSEALMQDKTSELLAELD
                     RA"
     gene            complement(5609..7048)
                     /locus_tag="VFB37_06285"
     CDS             complement(5609..7048)
                     /locus_tag="VFB37_06285"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HZO22098.1"
                     /translation="MLTPVILSGGAGTRLWPLSRELYPKQLLALTGERTMLQQTALRL
                     SGLPAVAPIVVCNEAHRFLVAEQLRQLHIEPRAVVLEPFGRNTAPAIALAALAALKTS
                     AGDPNAQDPALLVLPADHVIRDVPAFQAAVRVALGAAERAQLVTFGIVPNTPETGYGY
                     IQRGELAGAEKVHRIASFVEKPSLERAQAFLRSGDHFWNSGMFMFRASVYLQELERLA
                     PQIARHCRNAFENAKSDLDFIRIDAHDFEGCPGDSIDYAVMEKTADAVVVPLAAGWSD
                     IGSWSALHEASDADAHGNVSHGDVISEDSRGCYFHSESRLVAAVGLAEHVVVETKDAV
                     LVAPKSRVQDVKKLVARLREAGRYEHSLHREVFRPWGSYDSIENGERFQVKRLKVKPG
                     ASLSLQMHHHRAEHWIVVSGTARITRGDEVFLLEENQSTYIPIGVRHRIENPGMIPLH
                     IIEVQSGSYLGEDDIVRFDDRYGRQGTKN"
     gene            complement(7048..8400)
                     /locus_tag="VFB37_06290"
     CDS             complement(7048..8400)
                     /locus_tag="VFB37_06290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZO22099.1"
                     /translation="MRVTIFGSGYVGLVSGACLADAGNHVVCVDVDAAKIERLNRNEV
                     PIYEPGLDVLIKRNAEAGRLQFTTDAVKGVEHGLFQLIAVGTPPDEDGSADLRYVLAA
                     ARTIATHLGRYCIVVTKSTVPVGTADKVRSEIKTTLASRGTPVEFDVVSNPEFLKEGA
                     AIQDFMKPDRVVIGTDNPRTTELMRALYEPFTRNHDRLIVMDIRSAELTKYAANAMLA
                     TKISFMNELANIAERLGADIEKVRVGIGSDPRIGYSFIYPGTGYGGSCFPKDVQALIR
                     SAHEVGHKPGILDAVESVNQRQKQVLVHKMKAHFAAGLHGRTIALWGLAFKPNTDDMR
                     EAPSRAIIDMLLDDGARVRAYDPVAGTEAQRVYKDCKGFELARNAYEAAEGADALCIA
                     TEWQEFRSPDFERLREILKAPLIFDGRNLYDPAMLGRFGFTYYAIGRGRSEPATFGYT
                     "
     gene            complement(8516..10552)
                     /locus_tag="VFB37_06295"
     CDS             complement(8516..10552)
                     /locus_tag="VFB37_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZO22100.1"
                     /translation="MKDKYGAGGRKPAGVSAAETILALPRQQKRFIMLVADAIAIPTA
                     LWAALVLKFDRLDPVIGRHAAYYLVAVSSALIFFSVLGLYRAVIRFMGPRAMLNIATG
                     VTASVVVMAAYDRLLADAEIPLSAFAIYWALALLYALGSRFFVRYVIARTLHGHSATR
                     VAIYGAGDAGARLSSVLLGGPDFEPVAFVDDKKALQGSSINGLTVFAPEVLPQLVRER
                     QIDRVLLAMPSTSRRRRREILTSLEPLGLHVQSLPHLTDLIAGKARIDELRDVDVGDL
                     LGRDPVPPNPRLFGSCVRGKCVLVTGAGGSIGSELCRQIMHLAPSRLVLFEMSEIALY
                     TIERELNEIAVRDRLRVEVVPLLGNAHHRHRVREVLAAFSVQTVYHAAAYKHVPIVEH
                     NVVEGVHNNVISTWYTAEAAIETGVETFVLISTDKAVNPANVMGATKRLAELVLQALQ
                     ERSSGTRFCMVRFGNVLASSGSVVPLFQEQIRRGGPVTVTHPDVIRYFMTIPEAAQLV
                     IQAGSMAKGGDVFVLDMGRPVRIDDLARRLINLMGLAVRDADNPEGDIEIEYTGLRTA
                     EKLFEELLIGTNVTGTDHPMIMRAIEHRLPWQRMQQILNELLVALASFDCHGAVALLA
                     ESVAEYSQALELRDHVWTRKAVLPHAVDVKIADFAAKRRSSEAAAKPGHGQNPV"
     gene            complement(10855..>11680)
                     /locus_tag="VFB37_06300"
     CDS             complement(10855..>11680)
                     /locus_tag="VFB37_06300"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO22101.1"
                     /translation="CDAIVHLAGLAHQQASTASRLPEFLRINAEGTRLLARSASRARV
                     RRFVFVSSIAAVRTRSDTPVDDSTPCAPTDAYGCSKLEAERALLGELKNAPTDWCILR
                     PPLVYGPGNPGNMRRLLQLMSSGLPLPLASIRNRRSFIFVDNLVDALVRVLRHPPKVC
                     STYVLSDGSDFSTPELVTALAAAAGRRVRLFPVPVSVLGMLGRAGDAAGALLDKTVGL
                     STAVDRLVGSLPVDGTRFRQSFEWHPRINPDAALRQTARAPLGPAHTRPDSAQKST"
BASE COUNT         2103 a         3782 c         3844 g         1951 t
ORIGIN      
        1 catcggtcgc ctcgagccgc ccgtagcgaa tgttctccag cagggtccgg tgaaacaggt
       61 tcggatcctg cgggatgaga ctcagctgcg catgcagtga ttcctgcgtc atcgcgcgga
      121 tgtcctgacc gtcgatgaga atcgcaccct gctgcggatc atagaggcgc aggatcagac
      181 tgacgaaggt ggacttcccc gagcccgaga accccaccag cccgacgcgc tgaccggctg
      241 cgatctcgac gctcagattg cgaaacacgg gcctctcgga ggcatatgcg aaggtgacgt
      301 tgcggaactg gatccggccg cgcgtgatgg ccagcgcgcg ggcatcgggc cggtccacga
      361 tctcgtgcgg ccggatgatc gtgtgcacac cgttggtaac attccccacg tactcgaaga
      421 actcgagaaa gcgccggctc aggttgcgcg cctcgttgat gacgagcagc gtcgtggtgc
      481 tcgccaccac gaactccgcg accgagatct gcccactccc ccacaggcgc agtgcgtagt
      541 acagcacgcc gatcttgagg atcgccgcgg ccgtgtactg gaaccagcga acgcgctcgg
      601 aggaccagct cgagagtcgc actgcacgca actccttgcg gagctgccca tcgaggtact
      661 cacgctcata gcgcagacgc gcgaacagcc gcgtgctggt gaggttggtc acggcatcga
      721 cgaccttgcc cgtggtctcg ctgcgcgcgg cggccgctgc tacggcaaac ggccgcgtca
      781 gcgtggccag ccagaacgag gcacacacga aggcgagtgc ccacacgccc acgaatgtgg
      841 ccaggcccgg gtgcgcgtac gccagcatga cgatcgcgac cgcaaatacg atggccatcg
      901 gccagaagtc gaagatcacc gtccacagcg tctgcgagac tcccatggag gtctcgctga
      961 tccgatgggc gagcgccccg gcgaagtcat tgctcaggta gcgatgcgag tgatgctgca
     1021 ggtaggcgta catcgcgcgc gtcaccgcct gtctctggcg cggcccgatg aggatctgac
     1081 acatgccgcc ggcgcgactg aagatgacct cgccgatggc gagcgcgata aagagccaca
     1141 acggcccgcg caggaccgcc acgtaggaca tcgagtgctc gtgcgcactc gtcacggcct
     1201 tgatgatctg gccggtcgcg tacggaatca ggatggtgca ggtggagttg accgcctcga
     1261 gcagcaccat cgccagatac cagccgcgga agcgggcgat gaaatagcag atgaaccgga
     1321 acggtgtgtc cggcagcggc ggcgcaccgg gtgcgcgttg tgttggaccg gtcatgacag
     1381 ctgaagcagc ggcgaaacgc cgctcaatgc aacggctcga agccgtattt gctgaagagg
     1441 gcctgggcct cgcggctgtg caggatgaag tcttcgaatt cggccgccgc ggagtccgcc
     1501 ccgattgtca gtgcgatggg gtagacgatc ggggggtgtg tgttgggggg gaattgcgcc
     1561 acgatccgta cgcgtttttc ggccagggca tcggttcggt acacaatgcc cagcggcgcc
     1621 tcgccccggg caacgtaggc cagcgcagcg cgcacgttct ccgctccgac tatccgatcg
     1681 tacagcgagt tccacatgcc cagtttccgc agcgccgcgc gcgcgtagat gcccgcgggc
     1741 accgagtccg gatccccgat ggcgagctta ccctgtggcc cgagagcggc accgagattc
     1801 gcccccggcg caagcctcac gctgacccgg ctgtccgccg gcgcgatgag caccagctga
     1861 ttgccaacgg cctcgtgccg agagcccgga cgcagcagct ggcgagtatc cagatagtcc
     1921 atccattgca catcggcgga gaagaataaa tcggcctttg cccccgccac gatctgccgc
     1981 gccagtacgg agctcgccgc ataggaagcc ttgattggaa tcttcgtccg ctgcgtgtag
     2041 gccgcgctca tctcccccat cacctcggtg agcgacgcag ccccgaagac catgacggtc
     2101 ccggcggccc gagccggcac agtgcccgcc gcggcggtca gaaaggtcgc gacgacccac
     2161 aggagaagaa cccgaagagg cgatgcggat tgcatcatcc tggccccgac gggggcgctc
     2221 aatgccgctt ggggccgccc ggaatcgggg gcttgagtcc gccgggatag cctgtctggg
     2281 gtgatctcgt ggtactggat gtcgaagagc ccgccggggg gctcccaggg gttgcacccg
     2341 gagcagccga accctgggag gccggtgcag gcaggaccgg attggtcgaa gtcgtgctcg
     2401 tgcccggcgt gcccgctgcg gagggggctc cagcgggcgg ggcccggacc gtggcgctcg
     2461 gctggggggc ggagggcttg ggaggggcgg ccgaggtgcc cgctttggag gtcccggtaa
     2521 cctgaggcgg caaaacctcc accgacaggg tgaaggttcg cttctcggcg gcctgcagcg
     2581 cgcccacgtt gaccacctgg cgggtgactt cctcgccccg gtcgttccgg atggcgatga
     2641 tcacgaagta ctcgagcggc tgatcgaggg gcggatgacg aatgattccc tcgacgcgca
     2701 acgcaggcgt cgccgccacc tggcgcttcg ccgcctccga gtcgaagcgc aggtggtgca
     2761 tgcagcccgg gcagatgctg aggctttcga gaatcgtcgc cttgcagtgc ggacaggtgc
     2821 gcgtcttgcc cgtggtcacc gggcgagaag ccacgctcat gaaccggtgc cgtatccttt
     2881 ggtctcgggc ttgctgcgag actcgccgtc cgcccagccg aactcgacct ggccccgcat
     2941 gcgcgatccg gccgccacgg tcagtgagcc ggccttcaca tcgccgctga tcacgccgcc
     3001 ctcgagcagc tcgacccgct tggcgttgtc gatgttgccc agcagctcgc cgccgacgac
     3061 gaccacgcta gccttcacct ggccttccag gtgcgcaccg gtatcgagtg tcacgttgcc
     3121 atccacgtga acgtcgccct tgaagcggcc ggcgatccgg acgtggccgg agccgatgat
     3181 cttgccctcg atcgtgaggt ccgaggcgat gatggattct ttcatgtcca gacgctgcga
     3241 ttccttgact ggacggcgag caggatcggg gctgagcggc gcaacgttcg tcttctccgg
     3301 ctcacgtggg tgtggcagtg gccccgcagc cggaggcaga ttctgcgccg gcatcgggcc
     3361 gggtcctgtg ttcgtgtctt tccagagcgc catgagtagc ccccgtcggg ttgaaggaat
     3421 gaaaagttac cttccggtca tgctcgcggg cactgtcgcc ggaccgagac atgccaagcc
     3481 ggagcccagg ccgcacccgt gatcctggac ccaccgggta agcttgccgc cgatcgatcg
     3541 ccgagggaag cattttgagc ccgattgagg tccatctgat cgccgccgag cgtccgaact
     3601 ccgtgaaggc gggggcgttt gtaccacgct ttggtaaaaa aaagcgggtg cacagcgcac
     3661 atcgtgacac gggagaggct cgggaacagg cgacttccgg ggcgagagcc agggcgatgt
     3721 gggcggttcg ggtggggctg gcaccccggg gtccccaggg cgcacggccg acggatcagg
     3781 cgagcttgtt gggcctcagt gcctcgagca gcacctgcag atcgcagccc ttcgtgacat
     3841 aggcagccgc gccgcgccgc cgggcctcgc gggcgacatt ctcgctgtcc gccaggccgc
     3901 tgaagacgat gacgcgcaga tcgggcatgc aggtggtcag ctcttcgatc agcggcaggc
     3961 cactgccacc gtgcaaggtc aggtccagaa tgagcacgtt ggcggaggac tggcgggcca
     4021 tggggaccac ggaggcggca tccgacacct ggccgacaca gtgcatgtcc ggctcggtat
     4081 cgatgagctg gctcatcacc tcgctcagat cgcgatggtt ctccgcaatc agcacgcgaa
     4141 gcggcttctg ccctgggtcc aagacggttc ctgctgccga ttggtgcacg gctgcccgcc
     4201 ctcgcaaata ccgttcccga ccggcgcgcg cagtccgctg cggttgctat gcccggtcga
     4261 gttcggcaag caactcgctg gtcttgtcct gcatgagcgc ctcgctgccg cgtgactcga
     4321 cattcagccg cacgagcggc tcggtgttcg aggcacgcag attgaagcgc cagtcccgat
     4381 attcaatcga caagccgtcg gtgaggtcaa tggccagggc gcccttctcg tagcgctggc
     4441 gcacgcgagc gaggatggcc tgaggctccc ccgccaggcg gcggttgatt tcgccgctcg
     4501 caggaaacag gcgcatgcgc tcgcccagga gctccgagag cgtctggccg cgctggccga
     4561 tctcctggag caccagcagc cagggaatca tcccgctgtc gcagtaggaa aagcgccgga
     4621 agtagtgatg cgcgctcatc tctccgccgt atgccgcatc cacctcgcgc atcttctgct
     4681 tgatgaaggc gtgcccggat ttgcacagga ccgggacgcc ccccttgctg cgaacgatat
     4741 cgagcgtatt ccaggtcaag cgcggatcgt gcacgatgcg cgcgccgggc tcggtacgca
     4801 gaaaggcctc cgccagcaat ccgacgaggt agtacccctc gatgaagcga ccctgctcgt
     4861 caaaaaagaa acaccggtcg taatcgccgt cccaggccaa tcccacatcg gcaccgctgc
     4921 tgcgcaccac atcggccgta gccgcctggt tctcctccag catcggattg gggatgccat
     4981 tgggaaaggc gccgtcgggc tcgtgatgca ccttgacgaa ctcgaacggc aggtgaggct
     5041 cgatctggtc gatgacgagc cccgcaccgc cattacccgc attgacgacc accttcaacg
     5101 ctcgcagctt cgcccgatcg acataactca gcaggtgctc gagatagcgg gggcggatat
     5161 ccagcggctg ctcggagccc ttgcgtcccg ccccgggcgg gggctgaccc gactcgatca
     5221 gcgcgcgcat ctggcgcagg ccggtgtcgg cactgatggg ccgggcctgc tcacgcacga
     5281 acttcatgcc gttgtaatcg gatggattgt ggctggcggt gaccatgatt ccgccgtcga
     5341 ggccttcggc gaaggtggcg aaatacgcgc cttccgtgcc ccacaagcca atgtccttga
     5401 cgctgcaacc gctctcgatc agtcctcgct tgagcgctgc ggccagtgtc ggactcgaga
     5461 gcctgatgtc gtgtcccaca gcgaccgtac ccggcttcac gaacgcgcag tacgctcgac
     5521 cgatgtcata ggcgagctgc tcattgatct cgtcgggaat gcggccgcgg acgtcgtacg
     5581 ccttgaatgc gttcaggttc cgcatgcgtc agttcttggt gccctggcgc ccgtagcggt
     5641 catcgaagcg gacgatgtca tcctctccca ggtacgagcc ggattgcacc tcgatgatgt
     5701 gcagtgggat catgcccgga ttctcgatcc ggtgccggac gccgatcgga atgtaggtgg
     5761 attgattctc ctcgagcagg aacacctcat cgccgcgggt gatgcgcgcc gtccccgaca
     5821 ccacgatcca gtgctcggca cggtgatggt gcatctgcag ggagagcgag gcgcccggct
     5881 tgaccttgag gcgcttcacc tggaagcgct cgccattctc gatactgtcg tagctgcccc
     5941 aggggcgaaa gacttcgcga tgcagggagt gctcgtagcg tccggcctcg cgcaatcgcg
     6001 cgacgagctt cttcacatcc tgcacacggg atttgggcgc gaccagcacc gcatccttcg
     6061 tctccacgac cacgtgctcg gccaggccta ccgcggcgac caggcggctc tcggaatgga
     6121 agtagcagcc gcgcgagtcc tccgagatca catcgccatg cgagacgttg ccgtgcgcgt
     6181 cggcatcact ggcttcgtgc agcgccgacc aggacccaat atcgctccag ccggcagcga
     6241 gcggcaccac caccgcatcg gcagtcttct ccatcaccgc gtagtcgatt gaatcgccgg
     6301 ggcagccctc gaagtcgtgc gcatcgatgc gaatgaaatc gaggtcggat ttcgcattct
     6361 cgaaggcgtt gcggcaatgt cgggcgatct gcggcgcaag gcgttcgagc tcctgcaggt
     6421 agacgctggc gcggaacatg aacatgccgc tgttccagaa atgatcgccg gagcgcagga
     6481 aggcctgagc ccgctcgagg ctcggcttct cgacgaagct ggcgatgcga tgcaccttct
     6541 cggcaccggc aagctcaccg cgctggatgt aaccgtagcc ggtctcggga gtgttgggca
     6601 cgatgccgaa agtcaccagt tgggcccgct cggccgcgcc gagggcaacg cgcacggcgg
     6661 cttggaaagc gggcacgtcc cggatcacgt gatccgctgg caaaaccagc agcgctggat
     6721 cctgggcgtt gggatcgccg gccgaggtct tgagggcggc cagcgctgca agcgcgatcg
     6781 ccggcgcggt gttacgtccg aaaggctcca gcaccacggc tcgcggctcg atgtgcaact
     6841 ggcgcagctg ctcggcgacc agaaaccgat gcgcctcgtt gcagaccacg atcggtgcga
     6901 ccgcaggcag ccccgagagt cgcagcgccg tctgttgcaa catcgtgcgc tcgccggtga
     6961 gggcgagaag ctgcttggga tagagctcgc gcgacagcgg ccacagccgc gtgcccgctc
     7021 cccccgacag gatgactgga gtgagcatca ggtgtacccg aatgtggccg gctccgagcg
     7081 gccgcggccg atcgcgtagt aagtgaaacc aaatcgcccc agcatggccg gatcatagag
     7141 gttccggccg tcgaaaatga gcggggcctt caagatctcg cgcagccgct cgaaatccgg
     7201 gctgcggaac tcctgccact cggtggcaat gcacagggca tcggctccct cggccgcctc
     7261 ataggcattg cgggcgagct cgaagccctt gcagtccttg tagactcgct gcgcctccgt
     7321 ccccgcaacc gggtcgtagg cccgcactcg cgctccgtca tcgagcaaca tgtcgatgat
     7381 ggctcgcgag ggagcctcgc gcatatcgtc ggtgttgggc ttgaatgcca gtccccacag
     7441 ggcaatggtg cggccgtgca gcccagcggc gaagtgggcc ttcatcttgt ggaccagaac
     7501 ctgcttctgg cgctggttga cgctctcgac ggcgtccagg attcccggtt tgtggccgac
     7561 ctcgtgcgcc gagcggatca gcgcctgcac gtccttgggg aagcaggagc caccataacc
     7621 ggtgccgggg tagatgaaac tgtaaccgat ccgcgggtcc gagccgatgc cgacacgcac
     7681 cttctcgatg tcggcgccaa gacgctcggc gatgttggcc aactcgttca tgaagctgat
     7741 cttggtggcg agcatcgcgt tcgcggcgta cttcgtcagc tccgcggagc ggatatccat
     7801 cacgatcagg cgatcgtgat tgcgggtgaa gggctcgtag agcgcccgca tcagctcggt
     7861 ggtgcgcggg ttgtccgtgc cgatcacgac gcgatcgggc ttcatgaaat cctgaatcgc
     7921 cgcgccctcc ttcaggaatt ccggattgga gaccacatcg aactcgacag gagtgccgcg
     7981 actggcgagc gtcgttttga tctcgctgcg caccttgtcg gcggtgccaa ccggcacggt
     8041 cgacttggtg accacgatgc agtagcggcc gagatgcgtg gcgatggtgc gggcggcggc
     8101 cagcacatag cgcaaatccg ccgagccgtc ctcatccggg ggcgtcccga cggcgatgag
     8161 ctggaaaagc ccgtgctcga cgcccttgac ggcatcggtg gtgaactgca ggcgccccgc
     8221 ttccgcgttg cgcttgatga gcacatccag cccgggctcg taaatgggta cctcgttgcg
     8281 gttgaggcgc tcgatcttgg cggcatccac gtccacgcaa accacatgat tgccggcatc
     8341 agccagacag gcaccgctga cgaggcctac gtacccggag ccgaagatcg tgacacgcat
     8401 ttagataagt tcttgagtta aaagggagaa tggccgggag agtaaagcag gccgcggcgg
     8461 gcctcaagcg cgcctggcgc gtattgtgcg gcaagtcggc cgccggggag aaccttcaga
     8521 ccgggttctg gccgtgcccg ggctttgcgg ccgcttccga gctgcggcgc ttggcggcga
     8581 agtccgcgat cttgacgtcc accgcgtggg gcaacacggc cttgcgcgtc cagacatggt
     8641 cgcgcagctc gagggcctgg ctgtattcgg ccaccgattc ggcgagcaac gccaccgcac
     8701 cgtggcaatc gaagctcgcc agtgccacca gcagctcatt gaggatctgc tgcatgcgct
     8761 gccacggaag gcggtgctcg atcgcgcgca tgatcatcgg gtggtcggtg ccggtgacat
     8821 tggtgccgat gagcagctcc tcgaacagct tctccgcggt ccgcaacccg gtgtactcga
     8881 tctcgatgtc gccttccgga ttgtcggcat cgcgcacggc aagtcccatc agattgatga
     8941 ggcgccgcgc cagatcatcg atgcgcacgg gccggcccat gtccagcacg aacacatcgc
     9001 cgcctttggc catcgagcct gcctggatca ccagctgcgc ggcctcggga atggtcatga
     9061 agtaacggat cacatccggg tgggtcacgg tgacgggacc gccgcgacgg atctgctcct
     9121 gaaacagcgg caccacggag ccggatgagg cgagcacgtt accgaaacgg accatgcaga
     9181 agcgggtgcc gctcgagcgc tcctgcagcg cctgcaacac gagctcggcg aggcgcttgg
     9241 tcgcacccat cacattcgcc ggattgacgg ccttgtcggt ggagatcagg acgaatgtct
     9301 cgactccggt ctcgatcgcg gcctcggccg tgtaccaggt gctgatgaca ttgttgtgaa
     9361 cgccctcgac cacgttgtgc tcgacgatcg ggacgtgttt gtaggcggcg gcgtgataga
     9421 ccgtctgcac gctgaaagcg gcaagcactt cgcgcacccg gtggcgatga tgcgcattgc
     9481 cgagcagcgg cacgacctcc acccgcaatc ggtcgcgcac ggcgatctca ttcagctcac
     9541 gttcgatggt gtacagggcg atctcggaca tctcgaacag gacgagccgg ctgggcgcga
     9601 ggtgcatgat ctgccggcaa agctccgagc cgatcgagcc gcccgccccg gtgaccagca
     9661 cgcacttgcc tcgcacgcac gagccaaaca ggcgcgggtt gggcggcacg ggatcccggc
     9721 cgagcagatc gcccacgtcc acatcgcgca gctcatcgat ccgcgccttc ccggcgatca
     9781 gatcggtcag gtgcgggagc gactgcacgt gcagtccgag aggctccagt gaggtgagga
     9841 tctcacggcg gcggcgccgc gaagtggacg gcatggcgag caggacgcgg tcgatctgcc
     9901 gctcgcgtac gagctgcggc aacacttcgg gcgcaaacac cgtgagccca ttgatgctgc
     9961 tgccctgcaa ggctttcttg tcgtccacga aggccacggg ctcgaagtcc gggccgccga
    10021 gcagcaccga ggacaggcgc gctccggcat cgcccgcgcc atagattgcg acccgggtag
    10081 ccgaatgccc gtgcagcgtc cgcgcaatca cgtagcgcac gaagaagcgg ctgccgagcg
    10141 cgtatagcag tgccagcgcc cagtagattg cgaaagccga aagcgggatc tcggcgtccg
    10201 cgagcagccg gtcgtatgcg gccatgacca caaccgaagc cgtgacgccg gtggcgatgt
    10261 tcaacatcgc ccgcggcccc atgaagcgga tcaccgcccg gtacagcccg aggaccgaaa
    10321 agaaaatgag ggccgagctc accgccacca ggtagtaggc ggcatgacgg ccgatgacgg
    10381 gatcgagccg gtcgaacttc aggaccaggg cagcccacag cgcagtgggg attgcgatcg
    10441 catccgcgac cagcatgatg aagcgttttt gctggcgagg cagggccagg atggtctcgg
    10501 cggccgagac acccgctggt ttgcgtcccc cggccccgta cttgtccttc atgcgatctc
    10561 ccctggcgcc cgagacagcc cactgctccc gttgggctgc aaccgttacc ttatgctcgg
    10621 gcgatcaaaa acgacgcgcg attgtaaagc aaccggacgg accaatacag attcaaccac
    10681 ttggaacgca ccacccacac tggcaggaac gctgatcgct caggtgccag caagagtggg
    10741 acccaaataa gttgtttgcc gccactcacc gctggcgccg cacgctgaaa cgtgcgctgc
    10801 gtctccattc atcagcgccg actctcgcac tcactcccaa cgagggtagc aggcttaagt
    10861 tgacttctga gcagagtccg ggcgcgtgtg cgcaggtccg agcggcgctc tcgctgtctg
    10921 ccgcagggct gcgtcgggat ttatgcgagg atgccattca aacgactggc ggaaccgggt
    10981 tccatcgact ggcagggagc ccaccagccg atcaacggcg gtggaaagtc ccactgtctt
    11041 gtcgagcagc gcgcccgcgg catcgccggc gcgtccgagc atgcccagca ccgatacggg
    11101 gactggaaac agccgcacgc ggcgcccggc tgcagccgcc agcgccgtca cgagctccgg
    11161 agtggagaaa tcgctgccat cgctcagcac ataagtcgag cacaccttcg gcgggtgccg
    11221 caacaccctc accagggcat cgaccagatt gtcgacgaaa atgaagcttc gccggttgcg
    11281 gatcgaggcg agcggcagag gcaatccgct gctcatgagt tgcagcaacc gcctcatgtt
    11341 gccgggattg cccggaccgt agacgagcgg aggtctcagg atgcaccagt ccgttggggc
    11401 attcttcagc tccccgagca gcgctcgctc cgcttcgagc ttgctgcatc cgtaggcatc
    11461 ggtgggcgcg cagggggtgg aatcatccac cggcgtgtcg cttctggtac ggaccgcggc
    11521 aatcgagctg acaaagacga atctgcgtac cctcgcccgg gatgccgacc gggcgagcag
    11581 tcgggtgccc tcggcattga tgcgcaggaa ttccggcagg cgagaggcgg tactcgcctg
    11641 ctgatgcgcc agacccgcaa gatgcacaat ggcatcgcag
//
LOCUS       DASRHN010000162        21524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1040377, whole genome shotgun sequence.
ACCESSION   DASRHN010000162 DASRHN010000000
VERSION     DASRHN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21524
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040377"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFB37_06305"
     CDS             complement(<1..959)
                     /locus_tag="VFB37_06305"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZO22102.1"
                     /translation="MNKALTERLRADLAALKDQGLYKGERVLAGPQGGVVRTEDQDVV
                     NLCANNYLGLANHPAMREAAHRALDRFGYGMASVRFICGTQSVHKQLEERLSRFLGTE
                     DTILYSSCFDANGGLFETLLDEQDAVISDALNHASIIDGIRLCKAQRHRYANNDMAEL
                     ESCLKQTQGARTRLIATDGVFSMDGIIANLRGICELADRYDALVMVDDSHASGFMGPG
                     GRGTPEHCGVANRIDIVTSTLGKALGGGSGGFISSRREVIEWLRQRSRPYLFSNSIPP
                     VVAGVSLRVLDLLEHSPELRTRLFENTRYFREGLEKAGFSLKPG"
     gene            982..1389
                     /locus_tag="VFB37_06310"
     CDS             982..1389
                     /locus_tag="VFB37_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZO22103.1"
                     /translation="MGDTRTDETPGARRVDRWLFAVRLFKSRSLAATAVSGGRVHLNG
                     ERVKPSHGVRPGDAVSFMRGTVEFECTVVAIPLRRGPAREAVRCYDETPASQARGAEH
                     AARMRMAAALTPRPDERPEKHDRRLLRRLRGRS"
     gene            1460..2827
                     /locus_tag="VFB37_06315"
     CDS             1460..2827
                     /locus_tag="VFB37_06315"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HZO22104.1"
                     /translation="MTLSVFDVFKIGIGPSSSHTMGPMRAAREFALELKRDGLLGATT
                     QIAVRLYGSLALTGAGHGTDRAVLAGLEGAEPESVDPDSIESILKRVRAAGRINLLGE
                     HPIAFDEPMQLLFMRHERLPRHSNGMRFTALDEQLKTLRETDYYSIGGGFILRNDEAE
                     AREASSHAAPPYPFSSGAELLALCREHGLELYELMLANERTWRTAEQIHSGLLHLWQV
                     MQGCVERGFRQTGVLPGALKVRRRAPRMYRMLTESHAPRPLEVMDWVNAFALAVNEEN
                     AAGGRVVTAPTNGAAGIIPAVLHYYVRFEAGANEQGVLRFLLSAAAIGMLYKRNASIS
                     GAEMGCQGEVGVACSMAAAGLVAALHGTNEQIENAAEIGMEHNLGLTCDPVAGLVQIP
                     CIERNAMGAVKAINAARLAMRGDGSHKVSLDQVIATMRQTGADMSTIYKETSQGGLAV
                     NVPEC"
     gene            complement(2877..3389)
                     /locus_tag="VFB37_06320"
     CDS             complement(2877..3389)
                     /locus_tag="VFB37_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZO22105.1"
                     /translation="MKRLTLMRHGNAEWKDPEVPDFDRTLSRRGSSEAEVMARRLSEL
                     GLVPTLLLVSPARRAQQTADIVVRELGIPPRHLRSQESLYLAEAQDILGVVSATGPRI
                     PHLMIVGHNPGLSEVVRLLSRELPAHELGTGAVCSLTFDARTWSAVDAANLRETQCET
                     PVRGLFRMFA"
     gene            complement(3386..3703)
                     /locus_tag="VFB37_06325"
     CDS             complement(3386..3703)
                     /locus_tag="VFB37_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZO22106.1"
                     /translation="MSQEWVDVGSADSLAESQLLSAEIDGLALLVVRCGPELYAIEDR
                     CTHDGEALEGGEVDDCQIICPRHGAHFCVRTGEALTPPAYEPLRTFFVRVENGRILVE
                     NPE"
     gene            complement(3720..4271)
                     /gene="sufT"
                     /locus_tag="VFB37_06330"
     CDS             complement(3720..4271)
                     /gene="sufT"
                     /locus_tag="VFB37_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HZO22107.1"
                     /translation="MRSHENEPFVVNREVPAVIVPAGVEVKLRPGQAGYITQALGGSF
                     TVYIDGNLLRISGEHADAIGKEVVKAPELPPNATEEDVKNLAWEQMKTCFDPEIPINI
                     VDLGLVYECIVTANPDGTRCVEITMTLTAPGCGMGEVLVEDVKEKVERIPTVSTANVE
                     LTFDPPWNQSMMSEAARLQTGMF"
     gene            complement(4274..4717)
                     /locus_tag="VFB37_06335"
     CDS             complement(4274..4717)
                     /locus_tag="VFB37_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZO22108.1"
                     /translation="MDLKDLYRDVILDHNRQPRNFGRLEPADASAEGHNPLCGDRLTL
                     FLRLDGDRVEDVRFEGKGCAISTASASLMTEAVKGKDRTAIAELFSRVYALLTRQDAQ
                     ADASLGKLAALGGVREFPARVKCASLCWHTLNAALAESGSMVSTE"
     gene            complement(4717..5970)
                     /locus_tag="VFB37_06340"
     CDS             complement(4717..5970)
                     /locus_tag="VFB37_06340"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZO22109.1"
                     /translation="MATVATHAVTPPTLDAERVRRDFPILSRTINGRPLVYLDSGASA
                     QRPTAVLRAVEEYETHSHANVHRGVHALSQAATEAFEGARESARLFINARSTHEIIFT
                     RGTTEAINLVSQAYARPRFGAGDEIIVTALEHHANIVPWQMVCEQTGCSLKVAPINER
                     GELRFEEYLKLLSARTRLVAVAHVSNALGTILPVKRIVEAAHARGAVVVVDGAQAVPH
                     TRVDVRALGCDFYAFSGHKMYGPTGIGVLYGREELLEAMPPWQGGGDMILSVSFEKTT
                     YNALPAKFEAGTPNISGAVGLAAAMDYLESLGIEAVAAHEHRLVELATKELERIPGIT
                     IVGTAAQKASIVSFTMQGVHPHDLGTILDHEGVAVRTGHHCAMPLMSFLGLPATVRAS
                     FACYNTDRDVASLVAAVRKAREVFS"
     gene            complement(5971..7275)
                     /gene="sufD"
                     /locus_tag="VFB37_06345"
     CDS             complement(5971..7275)
                     /gene="sufD"
                     /locus_tag="VFB37_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HZO22110.1"
                     /translation="MINRISEEFAAAASHLPAAMLSAERRRSALQALETEGLPTSREE
                     NWKYINLRPLETVRFAPSRDQRAAALLTSAELPPPIQGYARYTFVDGVFAPALSAATD
                     RAGVRVRSMSRDCGSPAESDAAPVRWPNDLRFALLSEAFATDGASIVVAEGADCPSCV
                     ELVFVATAASNTAASYPRVEIQVAAQARVGLIERHVSIGSDANFINALVKIDLARAAF
                     VQHHRVQQTGAHALWYDTLSAHLAEASRYQLFCVNLGAQTARSTVNIQLNGERAEVDL
                     YAVAVGDRRQVHDTYAYVEHLAPRARTGQYFRGISSGRARVAFNGKIIVSKDAQGTDS
                     RQSLRGLLAGPDAEIDVRPQLEIYTDDVRCSHGATAGKLDDEMLFYMLSRGLERDAAQ
                     RLLKWAFLEDVVAKIEVPELRRQIEHSLAGQLGESAALQELL"
     gene            complement(7284..8126)
                     /gene="sufC"
                     /locus_tag="VFB37_06350"
     CDS             complement(7284..8126)
                     /gene="sufC"
                     /locus_tag="VFB37_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZO22111.1"
                     /translation="MTGTTSMLKIDNLHTAVGGNEVLNGLDLEVPTGEVHAIMGPNGS
                     GKSTLAHVLAGRPGYQITAGSVTLDGRDLLSLEPEERAHEGLFLGFQYPVEIPGVNNV
                     YLLKAALNAQRKHRGLPEVDAFEFLTLVREKMQLMRIDESFLSRGVNEGFSGGEKKRN
                     EILQMLILEPRLAILDETDSGLDIDALKLVASGVNALRRPERSIVLITHYQRLLEHVV
                     PDRVHVLVGGRIVRSGDRTLALELERRGYDWLIEATPAAIAQAAGGPSRRAAAGTGSD
                     GRVP"
     gene            complement(8141..9589)
                     /gene="sufB"
                     /locus_tag="VFB37_06355"
     CDS             complement(8141..9589)
                     /gene="sufB"
                     /locus_tag="VFB37_06355"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZO22112.1"
                     /translation="MSETVDQLEGLLARGYRHGFVTDIDSDTVPPGLDESVVRLISRK
                     KNEPSFLTDWRLKALRHWLQQPQPRWAHVRIAPIDYQAISYYSAPRAKTDGPKSLAEV
                     DPKLLETYAKLGVPLHERARLAGVAVDAVFDSVSVATTFKERLAVAGVVFCPFSEAVQ
                     THPELIEQYLGSVVPYSDNFFAALNSAVFSDGSFVYVPKGVRCPMELSTYFRINAAKT
                     GQFERTLIIADEGATVSYLEGCTAPMRDENQLHAAVVELIALDQAYIKYSTVQNWYPG
                     DANGVGGIYNFVTKRGECRGRHSRISWTQVETGSAITWKYPSCVLRGEGSIGEFYSVA
                     TVNNYQQADTGTKMIHIGRDTRSTIVSKGISAGHGQNTYRGLVKILPSAAGARNFTQC
                     DSLLMGSECGAHTFPYVEVKNPSATVEHEASTSRISDDQLFYCLARGIPREDAVNMIV
                     SGFCKSVFKELPMEFAVEAQALLAVSLEGAVG"
     gene            complement(9582..10076)
                     /locus_tag="VFB37_06360"
     CDS             complement(9582..10076)
                     /locus_tag="VFB37_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HZO22113.1"
                     /translation="MLRISRLTDYATVILAALAEEPGQVRTATALAERTRIAAPTVSK
                     LLKQLQRAGLVASTRGLHGGYQLARPATQISAATILDALEGPMALTDCSAGHGNCGIE
                     DTCRVGRVWQRLNHAIRRSLSDVSLAQLAGLDSTQVRLPSLEREMKTATARGAGRPGV
                     TRHE"
     gene            10343..11365
                     /locus_tag="VFB37_06365"
     CDS             10343..11365
                     /locus_tag="VFB37_06365"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZO22114.1"
                     /translation="MNRGELARVAGAMVVKGKGILAADESSGTIKKRFDSIKLESTEE
                     HRRTYREMLFTAPGASEYISGVILYDETIRQKTQDGTPFPEYLSRKGMIPGIKVDTGA
                     KPLAGSQGETITEGLDGLRERLVEYHKLGARFAKWRAVIDIGEGIPTSYAIGVNAHAL
                     ARYAALCQEQQIVPIVEPEVLMDGSHSIERCEEVTSAVLQQVFDELFEQRIYLEGMVL
                     KPNMVISGKKAANRAAPEQVAEATLRCLKRHVPPAVPGIAFLSGGQSSTEATLHLSLM
                     NKLGPLPWSLTFSYGRALQDDALKSWGGKSGQFSAGQQQLSKRAKLNSLATAGRYTSA
                     METQAA"
     gene            11385..12233
                     /locus_tag="VFB37_06370"
     CDS             11385..12233
                     /locus_tag="VFB37_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO22115.1"
                     /translation="MTKADSPMDASPATADDVVVDVRDVHYAVEQRPIFSGLNIQVRR
                     GRITAVMGPSGTGKTTLLRLITGQIVASQGRVVVLGQDIAGARAAEIYALRKRMGMLF
                     QNGALLTDMSVFENVAFPVREHTDLPEPLIRRLVLTKLQSVGLRGAAELFPAELSGGM
                     ARRVALARAIVMDPELLIYDEPFVGLDPISLGMILRLIKQMNEALGITSVVVSHDVQE
                     LATIADDSYLLSGGRVVGAGSPQELARSDSQVVHQFMTGAAEGPVAFHYPAPDYAEQL
                     LTAEQA"
     gene            12230..12994
                     /gene="mlaE"
                     /locus_tag="VFB37_06375"
     CDS             12230..12994
                     /gene="mlaE"
                     /locus_tag="VFB37_06375"
                     /inference="COORDINATES: protein motif:HMM:NF033619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HZO22116.1"
                     /translation="MTVARDDGFLLQGIRRMGSVLLFLFSLAAQCVPALARPRLIVMQ
                     IYNAGARSLIIIMLSGLFVGMVLGLQGFELLQRFGSEEALGTAAALGLIKELGPVVTA
                     LLFAGRAGTALTSEIGLMRATDQLTAMEVMAVDPLRYVAAPRFLGGIVAMPLLAAIFS
                     LIGLFGAQLIGVQLMGVDKGAFWSQMQDAVGMRDVTEGIVKSLVFGISCSLIAVHEGY
                     TAEPTAAGVGLATTRTVVASSVLTLLLDYVLTAEFL"
     gene            13025..13558
                     /gene="mlaD"
                     /locus_tag="VFB37_06380"
     CDS             13025..13558
                     /gene="mlaD"
                     /locus_tag="VFB37_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HZO22117.1"
                     /translation="MRANRTLEIGTGLFVLLGFAALFFLTTQLPASGLKLGGAKSGYH
                     VTAEFDNIGDLKVGSPVTMAGVRIGEVDGIHFDPRSYRAVVGLRIDTQYNQIPDDSFA
                     SIQTQGLLGGKYIGFSPGGSDTFLKNGSRVEQTQSAIVLESLINKLFASFADKSGSGG
                     ANSTSNGGSAKPEEQKK"
     gene            13555..14259
                     /locus_tag="VFB37_06385"
     CDS             13555..14259
                     /locus_tag="VFB37_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO22118.1"
                     /translation="MRPVTIIVAGALFAAAGSGVPAAAAPAPPAADSPAPTPQAQGAQ
                     SQESPSELVQQTAQGILKDLEANRAAYKQDPSKIAALVDKYLLPHFDTEYSAKLVLAQ
                     NWRTATPDQRKRFIDAFYHSLITNYGAYLADFTADRLKVFPSTVEPGADHATVRTEVR
                     RDNGDRIAVNYSLHMTPQGWKAYDVNIDGISYVKSYRDDFGSQIQQQGLDSVISRLER
                     GEKPGDIAKTASGKKS"
     gene            14291..14626
                     /locus_tag="VFB37_06390"
     CDS             14291..14626
                     /locus_tag="VFB37_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZO22119.1"
                     /translation="MKAANSAAPDSLELQEGSAGQFVARGPLTFATARRSRELGLSAF
                     GAARSREILVECAGISASDSAGMTVLLDWLSYAKQSGRALHFGNLPAQVQAIARISDV
                     LELLERGVS"
     gene            complement(14664..15578)
                     /locus_tag="VFB37_06395"
     CDS             complement(14664..15578)
                     /locus_tag="VFB37_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZO22120.1"
                     /translation="MPRTASLPLLAMLCLSLFGCATAPGTQPDPRDPFERVNRATYRF
                     NDAVDRAVLKPVALGYRKVTPRPVRTGVSNFIDNLSYTTTIVNDLLQLKFKPFLQDTG
                     RLVVNTTVGIGGLFDPATRIGLQKNEEDLGLTFGHYGAGPGPYIVIPFLGPSDLRDGI
                     GRVGDIFTDPRHYISNSWVSYGLWGLQVVDTRSRLLDSEKVLEGAYDRYAFLRNVYLQ
                     RRQYLVTGGETSKQQQQQEQQQYEDEKKILEETEGGEQPQTPPKANSPQGPQPGTPGQ
                     PTEPAPPRSNEPNPSPVPPQPPPPDQPH"
     gene            complement(15685..17163)
                     /locus_tag="VFB37_06400"
     CDS             complement(15685..17163)
                     /locus_tag="VFB37_06400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZO22121.1"
                     /translation="MRALILCLAAAFTTFAAAASAQQPVTPAPPDLPDMGSPAAAVLN
                     GTEEYQLGAMVIHQFREQNALIEDPEVSEYLNAVGDKLATQAPDGAPHFQFFAFRESA
                     INAFALPGGFICINYGLILATHNESELASVMAHEMAHVTQHHIARAIRAQGQQSMAAA
                     AAMLAAVLIGALSGGGGQAIEGGIAAAQGMAVQAQINFTRDNEAEADRVGIGFLAGAG
                     FDPRAMANFFETIGRVDGLAGTYIPAMLQDHPVTSVRIAEARSRAAQLPPVKVKESLN
                     YQLIRERLRVLTTPGDVDIEKQYAQKIAQGNNDIGTRYGEALALMADNRPVEAEKILD
                     KMTEQHEGLTLLHAALGQAQVKAGQTNDGVATLQHAVALFPRNVPVTVRYAEALMKAG
                     RAAEAHNVLLDLFNNVPPTPEQIRLTAMAASAAGDPGDAYFYMGEYQIAGGNLPLAAQ
                     QLQLALAAPHLTQVQRARYQARLDEVREYLANTRLRHVSNTQ"
     gene            complement(17218..18690)
                     /locus_tag="VFB37_06405"
     CDS             complement(17218..18690)
                     /locus_tag="VFB37_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZO22122.1"
                     /translation="MKRRSLSEPGPASAAAGELKAKARGDRRLFVLDTNVLMHDPTSI
                     FRFEEHDVYLPMVVLEELDAHKKGLSEASRNVRQVSRFLDDMMREATKEHIDQGLQLP
                     SGYSGNHGKKPSTGRLFFQTRELSSALPAGLPGQGSDNLILAQTLALQNGRPDARVIL
                     VSKDINLRIKAAVLGVHAEDYYSDKTLEDADVLYSGMSALPVDFWEKHGKDMRSWKEA
                     ERTFYEVTGPSVSEWQPNQGLYEDSPQGIEAMVRRVQPGTPATIELLRDYRTERHSVW
                     GITARNREQNFALNLLMDPEIDFVTVLGPAGTGKTLLTLAAGLMQTLETNRFVEIIMT
                     RVTIPLGEDIGFLPGTEEEKMEPWMGALMDNLEVLTSSQEGGTWGRAATNDLLRNRIK
                     IRSLNFMRGRTFLNRFIILDEAQNLTPKQMKALVTRAGPGTKVACLGNIAQIDTPYLT
                     EMTSGLTYAVNRFRGWAHSGHITLVRGERSRLADFASDTL"
     gene            complement(18668..19135)
                     /locus_tag="VFB37_06410"
     CDS             complement(18668..19135)
                     /locus_tag="VFB37_06410"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO22123.1"
                     /translation="MPPPKIGTRVKDFTLSASGGGTWGLKNAAGRKLVLYFYPKDMTS
                     GCTRESQDFRDLHAAFRKAGTMVVGVSRDSVRSHDRFTDKEKLPFPLLADEEEKLCKA
                     FDVIHEKSLYGRKYLGIERSTFLLDGAGVLRHEWRKVKVPGHAEEVLEAAKSL"
     gene            complement(19198..19728)
                     /locus_tag="VFB37_06415"
     CDS             complement(19198..19728)
                     /locus_tag="VFB37_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZO22124.1"
                     /translation="MKQHLAVSAIGGDRTGMVHELTRVISECGGNISESRMASLGTEF
                     AMLLLVSGNWHSLAKLETELKRLAETSSLSIHLKRTEPRTARTDMLPYSIDVVCLDQS
                     GIVSGLSGFFATRGIDIAEVSTRSYPAAHTGAPMFSVQMIVNVPSRIHVAHLREEFME
                     FCDSLNLDAILEPVKS"
     gene            19989..20867
                     /gene="dapA"
                     /locus_tag="VFB37_06420"
     CDS             19989..20867
                     /gene="dapA"
                     /locus_tag="VFB37_06420"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZO22125.1"
                     /translation="MFSGSLVAMVTPMRPDGALDLQAWERLIELHLSSGTRGLVVGGT
                     TGESATLSEAELWELTERACAQARGHIPVIVGAGTSSTAATVERARRLCELPIDGLLL
                     VTPAYNRPTQEGLYRHFAAVAAATRRPLILYNVPSRTSVDMLPATVQRLSQLPGIVAV
                     KEAVADVERIRELVAVCSHDFAVLSGDDATARSSILAGARGLISVTANVAPRQVSSMA
                     AAAMKGDAAEAGRLDAEVAALHRELFLEANPIPVKWALRRMELIQSGIRLPLTELSET
                     YRPAVLAALRAAGILN"
     gene            20887..21186
                     /locus_tag="VFB37_06425"
     CDS             20887..21186
                     /locus_tag="VFB37_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22126.1"
                     /translation="MIHRIGLAALLAALVILAGCHPLRSLRNVGGSCHDVQPYTKAQS
                     IAPLHIPAGLDAPDTSNSLQVPALNEPAPPPIGPKDPCLDRPPPFKVAKPATPQA"
     gene            21310..21401
                     /locus_tag="VFB37_06430"
     tRNA            21310..21401
                     /locus_tag="VFB37_06430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21344..21346,aa:Ser,seq:gga)
BASE COUNT         3731 a         6831 c         7090 g         3872 t
ORIGIN      
        1 ccgggtttca ggctgaaacc ggccttttcc agtccctcgc ggaagtaacg ggtgttctcg
       61 aacagccggg tgcgcaactc cggcgagtgc tcgagcagat ccagaacgcg caggctcacg
      121 ccggcgacca cgggcgggat gctgttggag aacagatacg gtcgcgagcg ctggcgcagc
      181 cactcgatca cctcgcggcg tgaggagatg aatccgccgc tgccgccgcc cagcgccttg
      241 ccgagcgtgc tcgtcacgat gtcgatccga ttcgcgaccc cgcagtgctc cggggtccct
      301 cgtccccccg gtcccatgaa gccgctggca tgcgaatcat cgaccatgac cagcgcatcg
      361 taacgatcgg ccagctcgca gatcccgcgc aggttggcga tgatgccatc catcgagaac
      421 acaccatcgg tggcgatgag gcgcgtgcgg gcgccctggg tctgcttgag acagctctcg
      481 agctccgcca tgtcattgtt ggcataacga tgacgctgcg ccttgcagag acggatgcca
      541 tcgatgatgc tcgcgtgatt gagggcatcg gagatcacgg cgtcctgctc gtccagcagc
      601 gtctcgaaca ggccgccgtt cgcatcgaag caggaggagt agaggatcgt gtcctctgtg
      661 ccgaggaatc ggctgaggcg ctcctcgagt tgcttgtgta cgctctgggt gccgcagatg
      721 aagcgcaccg aagccatgcc gtagccgaac cgatcgagtg cgcggtgcgc cgcctcgcgc
      781 atggccgggt gattggcaag cccgaggtag ttgtttgcgc acagattgac cacatcctga
      841 tcctctgtgc ggaccacgcc tccctgcggg ccggcgagga cgcgctcgcc cttgtagagt
      901 ccctggtcct tcaaggccgc gaggtcggct cgcagtcgct cggtcaaagc tttgttcatg
      961 cttgaattat actcgtgcag gatgggcgac acccgtaccg atgagacccc cggggcgagg
     1021 cgggtcgacc gctggctgtt tgccgtgcgg ctgttcaagt cccgctccct ggccgctacc
     1081 gcggtgagcg gcggccgcgt gcatctcaac ggggagcgcg tgaagccctc ccacggcgtg
     1141 cggccgggtg atgccgtaag cttcatgcgt ggcaccgtcg agttcgaatg cacggtcgtc
     1201 gcgatacctt tacggcgcgg gccggcacgt gaggcggtgc gatgttatga cgaaacgccg
     1261 gcgagtcagg cgcgcggggc cgagcacgcc gcgcgcatgc gaatggcggc ggcgctgacg
     1321 ccgcgacccg acgagcggcc ggaaaaacac gatcgccgct tgctgcgccg cctgcgcggc
     1381 cgcagttgag agcggggggc tcgtgaatag cgtgggaggc tcgtgaatag cgtgggaggc
     1441 tcgtaaacaa ggtgtgcgca tgacacttag cgtgtttgac gtcttcaaga tcggcatcgg
     1501 gccctcgagc tcccacacga tggggccgat gcgcgccgca cgcgagtttg cgctcgaatt
     1561 gaagcgcgac ggtctgctgg gcgcgacgac ccagatcgcc gtgcgtctgt acggctcgct
     1621 cgcgctgacc ggcgccggcc acggcactga ccgggcggta ctggcgggac tcgagggcgc
     1681 ggagcccgag agtgtcgatc cggacagcat cgagtccatc ctgaagcgag tccgcgccgc
     1741 cggtcgcatc aacctgctcg gtgagcatcc gatcgctttc gatgagccga tgcagcttct
     1801 gttcatgcgt cacgagcggc tgccgcggca ttccaacggc atgcgcttca cggcgttgga
     1861 tgagcagttg aagacgctgc gcgagaccga ctactactcg atcggtgggg gattcatcct
     1921 gcgaaacgac gaggcggagg cccgcgaagc gagctcacac gccgcgccgc cctatccctt
     1981 cagctccgga gcggagctgc tcgcgctctg tcgcgagcac ggcttggagc tgtacgagct
     2041 catgctggcc aacgagcgaa cctggcgcac cgcggagcag atccactcag gcctgttgca
     2101 cctctggcag gtcatgcagg gatgcgtgga gcggggcttc cgccagaccg gcgttctgcc
     2161 gggggctctc aaggtgaggc gcagggcccc gagaatgtac cgcatgctta ccgaaagcca
     2221 cgcgcccagg cccctggagg tgatggactg ggtgaacgcc ttcgcgctcg cggtgaatga
     2281 ggaaaacgca gccggtgggc gcgtcgtcac ggcacccacc aacggtgccg cgggcatcat
     2341 tccggcggtg ctgcactact acgtgagatt cgaggccggc gcgaacgagc agggcgtgct
     2401 gcggttcctg ctgtccgcag cggccatcgg catgctctac aagcgcaacg cttccatttc
     2461 gggggcagag atgggatgcc agggggaggt gggcgtcgct tgttcgatgg cggcggccgg
     2521 cctggtcgcg gcgctgcatg ggaccaacga gcagatcgag aacgccgccg agatcggcat
     2581 ggagcacaat ctgggtctga cgtgcgatcc ggtggccggc ctggtgcaga ttccctgcat
     2641 cgagcgcaat gcaatgggcg ccgtcaaggc catcaacgcc gcgcgtctag cgatgcgcgg
     2701 cgatggatcc cacaaggtgt cactcgatca ggtgatcgcg accatgcgcc agacgggtgc
     2761 ggacatgtcc acgatctaca aggaaacctc gcagggcggg ctggcagtca acgtgcctga
     2821 atgctgacgg gtgaggcgct tttcgaactc tagcgggccg cgaagccgcg gcgctctcag
     2881 gcgaacatcc tgaacaggcc acgcaccggg gtctcgcatt gggtctccct gagattagcg
     2941 gcatccaccg ccgaccaggt ccgggcatcg aacgtgagcg agcatacggc tccggtaccg
     3001 agctcatgcg ccggcagctc acgcgacaga aggcgtacca cctccgacag gccagggtta
     3061 tggccgacga tcatcaagtg cgggatgcgg ggcccagtcg cactcaccac gcccaggatg
     3121 tcctgagcct cggccaggta aagcgactcc tggctgcgca ggtgccgcgg tgggatccca
     3181 agctcgcgga ccacgatatc ggcggtctgc tgcgcacgac gtgccgggct gaccagcaac
     3241 agcgttggaa ccaggccgag ctcggaaaga cggcgcgcca tcacctcagc ttcggagctg
     3301 ccgcgccggc tcagggttcg atcgaagtcg gggacttccg ggtccttcca ctctgcattg
     3361 ccatggcgca tcagcgtcag acgcttcact cggggttctc caccagaatg cggccgttct
     3421 ctacacgcac gaagaaggtt cgcagcggct cgtaagccgg cggcgtgagc gcctcgccgg
     3481 tgcgaacgca gaagtgcgca ccatgtctcg ggcagatgat ctggcagtca tccacctcgc
     3541 ccccctccag agcctcgcca tcatgtgtac agcggtcctc gatcgcgtac agctcagggc
     3601 cgcagcgtac aacgagcaga gccaagccgt cgatttccgc tgacagcaat tgagactcgg
     3661 cgagcgagtc cgcgctgccg acatccaccc attcctggct cattcgcgcc ccggcccctt
     3721 cagaacatgc cggtctgcag ccgggcggct tcggacatca tcgactggtt ccagggcgga
     3781 tcgaatgtca gctcgacgtt ggcggtgctg accgtcggga tacgctccac tttttccttc
     3841 acgtcctcga cgaggacttc acccatgccg cagcccggag ccgtcagggt catcgtgatc
     3901 tcgacacagc gcgtgccatc cgggttcgcc gtcacgatgc actcgtacac cagaccgaga
     3961 tcgacgatgt taatggggat ctcaggatcg aagcacgtct tcatctgctc ccaggcgaga
     4021 ttcttcacgt cctcctcggt ggcattgggc ggaagctcgg gcgctttcac gacctccttg
     4081 ccaatggcat cggcatgctc gcccgagatg cgcagcaggt tgccatcgat atagaccgtg
     4141 aagctgccgc cgagcgcctg agtgatgtag ccggcctggc cgggcctcag tttgacctcg
     4201 acaccggccg gtacgatgac ggcgggaacc tcgcgattca ctacgaaagg ctcgttctcg
     4261 tgtgaacgca tggctactcc gttgagacca tgctgccgct ttcggccagg gcagcattca
     4321 gggtgtgcca gcagaggctt gcgcacttca cgcgagcggg gaactcgcgc accccaccga
     4381 gcgccgccag ctttccgagg ctcgcatccg cctgagcatc ctggcgtgtc aacagcgcgt
     4441 agaccctgga gaacagctca gcgatcgctg tcctgtcctt gcccttgacg gcttcggtca
     4501 tgagcgaagc cgacgccgtc gaaatcgcgc accccttgcc ctcgaagcgc acgtcctcca
     4561 cccgatcgcc atcgagacgc aggaatagcg tcagccgatc gccacagagc gggttatggc
     4621 cctcggcgct cgcatcggcc ggctcgagcc gtccgaaatt gcggggctgc cggttgtggt
     4681 cgaggatcac atcccggtag aggtccttca ggtccatcag ctgaatacct cgcgtgcctt
     4741 gcgcaccgcg gcgaccaggc tcgccacatc ccgatcggtg ttgtagcagg cgaacgaggc
     4801 tcgtaccgtg gccggcaggc caaggaagct catgagcggc atggcgcagt gatgtccggt
     4861 acgcactgcg accccctcgt gatccaggat cgttccgaga tcgtgggggt gcacaccctg
     4921 catggtgaag gagacgatcg aggctttttg cgccgcggtg cccacgatcg tgatcccggg
     4981 aatgcgctcg agctctttcg tggcgagctc gacgagccgg tgctcgtgcg cggccacggc
     5041 ctcgatcccc aggctctcga ggtaatccat cgcagcagca agacccaccg ccccggagat
     5101 gttaggggtt ccggcctcga acttggccgg cagcgcatta taggtcgtct tctcgaagga
     5161 gacgctcagg atcatgtcac caccgccctg ccacggcggc atcgcctcca gcagctcctc
     5221 acggccgtag agcacgccta tgccggtagg cccatacatc ttgtggccgg agaaggcgta
     5281 aaaatcgcac cccagcgcgc ggacatcgac cctcgtgtgc ggcacggcct gcgcgccatc
     5341 gaccactacg accgccccgc gggcatgtgc agcttcgacg atgcgtttga ctggcagaat
     5401 tgtaccgagc gcgttcgaga cgtgtgccac ggcgaccagc cgtgtacgcg ccgagagcag
     5461 cttcaggtac tcctcgaagc gcagctcgcc ccgctcgttg atgggcgcga ctttcagtga
     5521 gcagccggtc tgctcgcaga ccatctgcca cggaacgatg ttggcatgat gctcgagcgc
     5581 ggtgacgatg atttcgtcgc cggcgccgaa gcgcggtcgc gcataagcct gggacaccaa
     5641 attgatcgcc tcggtcgtac cgcgggtgaa gatgatttca tgcgtgctgc gggcattgat
     5701 gaagaggcgc gcgctctcgc gcgctccctc aaaagcctcg gtcgcggctt ggctgagcgc
     5761 gtgcacaccc cgatgcacgt tcgcatgcga atgggtctcg tattcctcca cagcgcgcaa
     5821 cacggctgtc ggccgctggg ccgaagcccc actgtcgagg taaaccagcg ggcggccatt
     5881 gatggtgcgg ctgagaatag gaaagtcccg acggacgcgc tcggcatcca gcgtcggcgg
     5941 ggttacggcg tgtgtggcga ctgtggccat ctacaacaac tcctgcagtg cggcggattc
     6001 gcccagctgg cccgcgagac tgtgctcgat ctgccgccgc agctcgggca cctcgatctt
     6061 cgcgaccaca tcctccagga acgcccattt gagcagacgc tgtgccgcat cgcgctccag
     6121 gccgcgcgac agcatataga acagcatctc gtcgtccagc tttccggcgg ttgcgccgtg
     6181 actgcaccgc acgtcatcgg tgtaaatctc gagctgcggt cggacatcga tctcggcatc
     6241 agggccggcc agcaggccgc gcaaagactg tcgcgagtcc gtgccctggg cgtccttgct
     6301 gacaatgatc ttgccgttga aagccacgcg tgcgcgtccg gaggagattc ctcgaaaata
     6361 ttggcctgtc cgcgcgcgcg gtgcgaggtg ctccacgtag gcatacgtgt catgtacctg
     6421 gcggcggtcg ccgacggcga cggcgtagag atccacctcg gcgcgctcgc cgttgagctg
     6481 gatattcacg gtggagcgag ccgtctgggc gccgagattg acgcaaaaga gctgatagcg
     6541 ggaagcctcc gccagatgcg ctgagagggt gtcgtaccag agtgcgtgag caccggtctg
     6601 ctgcacgcga tggtgctgca cgaaggctgc acgagccagg tcgatcttca cgagagcgtt
     6661 gatgaagtta gcgtcgctcc cgatgctgac gtgacgttcg atcagcccga ctcgcgcttg
     6721 ggcggccacc tgaatctcga cgcgcggata ggaggccgcg gtgttcgaag ccgcagtcgc
     6781 cacgaacacg agctcgacgc agctcgggca gtcggctccc tcggccacca cgatcgaggc
     6841 cccatcggtt gcgaatgcct cactcaacag ggcaaagcgc aaatcattcg gccagcgtac
     6901 cggggcggca tcgctttcag ctggggagcc gcaatcccgg ctcatcgagc gcactcgcac
     6961 acctgcccga tccgttgccg cggacagggc gggtgcgaac acgccatcca cgaacgtgta
     7021 gcgggcgtag ccctgaattg gcggcggcag ttcggccgag gtgagcagcg ctgcggccct
     7081 ctggtcccga gagggagcga accgaaccgt ctccagcggc cgcagattga tatatttcca
     7141 gttctcctcg cgcgaggtcg gaaggccctc cgtttcgagc gcctgcagtg ccgagcggcg
     7201 ccgctcagcg ctcaacatgg cagcgggcaa atggctcgcc gccgcggcga actcttccga
     7261 aatgcgattg atcatcgggc tgatcacggc acacgtccat ccgaacccgt gccggcagcc
     7321 gcgcgacggg aaggacctcc tgctgcctgc gcgatggccg cgggcgtcgc ctcgatgagc
     7381 cagtcatatc cgcggcgttc cagctcgagg gcgagcgtgc gatcgccgct gcggacgatc
     7441 cggccgccca ccagcacgtg cacccgatcg ggtacgacat gctcgagcag gcgctgatag
     7501 tgggtgatca gcacgatcga tcgctcgggc ctgcgcagcg cgttgactcc gctggccacg
     7561 agcttgagcg catcgatgtc gagtcccgag tccgtctcgt ccaaaatggc gagccggggc
     7621 tcgagaatga gcatctgcag gatctcattg cgcttcttct cgccgccgga gaatccctcg
     7681 ttgacacccc tcgacaggaa gctctcgtca atgcgcatca gttgcatctt ctcgcgcacc
     7741 agcgtcagga actcgaaagc gtccacctca ggcaaaccgc ggtgcttgcg ctgcgcattc
     7801 aaggcagcct tcaggagata gacgttgttc acgcccggga tctcgaccgg gtactgaaac
     7861 cccaggaaca atccctcgtg agcgcgttcc tcgggctcga ggctgagcag gtcgcggcca
     7921 tcgagcgtca cgcttcctgc ggtgatttgg tatcccggcc gcccggcgag cacgtgggcg
     7981 agggtgctct tgcccgagcc gttcggtccc atgatggcgt gcacttcgcc ggtcgggacc
     8041 tcgaggtcga ggccgttcag cacttcgttg ccgccgacgg cggtgtgcag attgtcgatc
     8101 ttgagcatgg atgtcgtgcc tgtcatgagt tgcgcagcgc tcaaccgacc gccccttcga
     8161 ggctcaccgc caggagtgcc tgggcttcca cggcgaactc catcggcagc tccttgaaca
     8221 ccgacttgca gaagccgctc acgatcatgt tgacggcgtc ctcgcgcgga atcccgcggg
     8281 ccaggcaata gaagagctga tcgtcgctga tcctcgaagt gctcgcctcg tgctcgaccg
     8341 tggcggaggg gtttttgacc tcgacgtacg ggaacgtgtg ggcgccgcac tcactgccca
     8401 tcagcagtga gtcgcactga gtgaaattcc gtgcgccagc ggcgctcggc agaatcttca
     8461 cgaggccacg ataggtattc tggccatgtc cggcggagat gcccttggag actatcgtgc
     8521 tgcgcgtgtc ccgcccgata tggatcatct tggtgccggt gtccgcctgc tggtaattgt
     8581 tcacggtagc gaccgagtag aactcgccga tcgagccctc gccgcgtagc acgcagctcg
     8641 ggtatttcca ggtgatggcg gatccggtct cgacctgtgt ccaggagatg cgcgagtggc
     8701 ggccgcggca ttccccccgc ttggtgacga agttgtagat gcctcccacg ccgttggcat
     8761 cgcccggata ccagttctgc acggtggaat acttgatgta ggcttgatcg agcgcgatca
     8821 gctccacgac cgccgcgtgc agctggttct catcacgcat gggcgccgtg cagccttcca
     8881 gatacgacac cgtcgcaccc tcgtccgcga tgatgagcgt gcgctcgaac tgtccggtct
     8941 tcgccgcgtt gatccggaaa taggtggaca gctccatcgg gcagcgaaca cctttgggca
     9001 cgtacacgaa tgagccgtcg ctgaacacgg cggaattgag tgccgcgaag aagttgtccg
     9061 agtaggggac gacactcccc aggtactgct cgatgagctc aggatgagtc tgcaccgcct
     9121 cggagaacgg gcaaaacacg acgccagcca cggccaggcg ctccttgaac gttgtggcca
     9181 ccgacacgct gtcgaacact gcatcgacgg ccacgccggc gaggcgggcg cgctcgtgca
     9241 gcggcacgcc gagcttggcg taggtctcaa gtaatttcgg gtccacttcg gccagactct
     9301 tggggccatc ggtcttcgcc cggggcgcgg agtagtacga gatggcctga tagtcgatcg
     9361 gggcgatcct gacgtgcgcc cagcggggct gcggctgctg cagccagtgc cggagcgctt
     9421 tcaaacgcca atcggtgagg aatgacggct cgttcttctt gcgtgagatc agccgcacca
     9481 cgctctcgtc caggccgggc ggaaccgtgt cggaatcgat gtcggtgacg aagccgtgcc
     9541 gatagccacg ggccagcagt ccttccagtt gatcgacagt ctcactcatg ccgggttact
     9601 cctgggcggc ccgcgcctcg cgcggtcgcc gttttcatct cacgctccag cgacgggagc
     9661 cgcacctgcg tcgagtcgag ccccgcaagc tgcgccaggc tcacgtcaga gagagagcga
     9721 cggatagcgt gattgaggcg ttgccagacg cggcctacgc ggcacgtatc ctcgatgccg
     9781 cagttgccgt gcccggccga gcagtcggtc agggccatcg gcccctcgag cgcatccagt
     9841 atcgtggcgg cgctgatctg ggtagcgggt cgggcaagct gataaccccc atgcaagccg
     9901 cgtgtggagg ccaccaagcc cgctctctgc aactgcttga gcagcttgct cacggtcggc
     9961 gccgcaatgc gggttcgttc cgccagagcg gtggcggtgc gcacctgacc cggctcctcg
    10021 gcgagagctg ccaggatcac ggtcgcgtag tctgtaagcc ggctgatgcg cagcatgact
    10081 tgagctccac ggcgggcttc aaaagtagga ctattttagt actgattaga cttcaatcca
    10141 agtgtgctgc cgcagggagg attacctggg gtcggggtgc gtggcgggca agcgcgggcg
    10201 cggtcgggtg cgcagagccc cggggcgcca ccgcgcgccg acggccctgg cgggccgcca
    10261 gggccgacca atttgatatt cttggcgcct gcactctcaa acgcctgatc ggcgtcactt
    10321 ggtatttccg gaggactgaa cgatgaaccg cggtgagctt gcgcgcgttg ctggcgcaat
    10381 ggtggtgaag ggcaagggta ttctggctgc cgacgagagc tccggaacca tcaagaaacg
    10441 cttcgacagc atcaagctcg agtccaccga ggagcatcgc cgcacctatc gcgagatgct
    10501 gttcaccgcc ccgggcgcct ccgagtacat cagcggcgtg atcctttacg acgagacgat
    10561 ccgccagaag actcaggatg gcacgccgtt cccggaatac ctcagccgca agggaatgat
    10621 ccccggaatc aaggtggaca cgggcgccaa gcctctggcc ggatcgcagg gcgaaaccat
    10681 caccgaaggg ctcgatgggc tgcgtgagag gctggtcgag taccacaagc tgggtgcccg
    10741 tttcgccaag tggcgggcag tcatcgatat cggcgagggg attccgacct cctatgccat
    10801 cggggtcaac gcccatgcac tggcgcggta cgcggcgctc tgccaggagc agcagatcgt
    10861 cccgatcgtg gagcccgagg tgctgatgga tggatcccac agcatcgagc gttgcgagga
    10921 ggtgacgagc gccgttctgc agcaagtgtt cgatgagctt ttcgagcaac ggatctatct
    10981 cgagggcatg gtgctgaagc cgaacatggt gatctccggg aagaaggccg cgaatcgcgc
    11041 ggcaccggag caagtcgccg aagcaacgct ccgctgcctg aagcgccacg tgccgcccgc
    11101 cgtcccgggc atcgcattcc tctccggcgg gcagtcctcg acggaggcca cgctgcacct
    11161 gtccctcatg aacaagctgg gcccactgcc ctggtcgctg actttcagct acggccgcgc
    11221 cttgcaggac gatgcgctca agagctgggg cggcaagagc ggccagttca gcgcgggcca
    11281 gcagcagctg tccaagcgcg ccaagctcaa tagcctggcc acagcgggcc gctacacctc
    11341 cgcaatggag acccaggccg cctaggagcg gtctcaacat tcgcgtgact aaagccgact
    11401 cgccgatgga tgcgtcgccg gcaacagccg atgacgtggt ggttgatgtg cgcgacgtgc
    11461 actacgcagt cgagcagcgc cccatattct cggggctcaa catccaggta cgccgcgggc
    11521 ggatcaccgc ggtgatggga ccgagtggta cgggaaagac cacgctgctg cggctgatca
    11581 ccgggcagat cgttgcgagc cagggcaggg tagtcgtcct gggtcaggac atcgcgggcg
    11641 cacgggcggc agagatctat gcgctgcgca agcgcatggg catgttgttt cagaatggcg
    11701 ccttacttac cgacatgagc gtgtttgaga acgtcgcctt cccggtacgc gagcatacgg
    11761 atctgccgga accgctgatc cgccggctgg tgctgaccaa gctgcaatct gtcgggctca
    11821 ggggcgccgc ggagctcttc ccggcggaac tctccggcgg catggcccgg cgcgtggcgc
    11881 tggcgcgagc catcgtgatg gacccggagc tgctgatcta cgatgagccg ttcgtcggac
    11941 tcgatcccat ctcgctgggc atgatcctgc ggctgatcaa acagatgaac gaggctctcg
    12001 ggatcaccag cgtggtcgtc tcgcacgacg ttcaggagct cgccaccatc gccgatgaca
    12061 gctacctgct ttccggcgga cgggtagtcg gtgccggttc gccccaggag cttgcgcgaa
    12121 gcgactccca ggtggtgcac cagttcatga ccggggccgc tgaagggccc gtcgccttcc
    12181 actaccccgc ccccgattac gccgagcaac tgctgacggc ggagcaagcg tgacggtcgc
    12241 cagggatgat ggctttctgc tgcagggcat ccgcaggatg ggcagcgtgc tgctgttcct
    12301 gttcagcctc gcagcccagt gcgttccggc gctcgcccgg ccccgcctga tcgtcatgca
    12361 gatctataat gcgggcgcgc gatcgctgat catcatcatg ctgtcgggac tgtttgtcgg
    12421 catggtgctc ggcctgcagg gtttcgagct gttgcagcgt ttcgggtccg aagaggcgct
    12481 cgggacggcg gcagccctcg gactgatcaa ggagcttgga cctgtggtga cggcgctgct
    12541 gttcgcgggc cgcgccggca ccgcgctcac ctcagagatc ggactgatgc gcgccaccga
    12601 ccagctgacc gccatggagg tcatggcggt tgatccgctg cgctacgtgg ccgcgccccg
    12661 cttcctgggc ggtatcgtgg ccatgcccct tctggcggcc atcttcagcc tcatcggact
    12721 gttcggcgct cagctcatcg gcgttcagct gatgggagtg gacaaaggcg cattctggtc
    12781 acagatgcag gacgcggtgg ggatgcgcga tgtgaccgaa gggatcgtga agagcctcgt
    12841 gttcggcatc tcctgcagcc tgatcgccgt gcacgagggc tacaccgcgg agccgacggc
    12901 cgcaggggtg ggcctcgcga cgacccgcac cgtggtcgcc tcgtccgttc tgacgctact
    12961 gctcgattac gtactgacgg ccgagttcct ctagctcagg ctgcatgact gccgaggacc
    13021 tgatatgcga gccaatcgga cactggaaat cggaacgggg ctgttcgttc tgctgggatt
    13081 tgcggcgctg ttctttctca ccacacagct gcccgcgagc gggctgaagc ttggcggtgc
    13141 aaaatccggc tatcacgtca cggccgaatt cgacaacatc ggggacctca aagtgggttc
    13201 gcccgttacg atggcaggcg tgcggatcgg cgaggtggac ggtatccact tcgatccgcg
    13261 atcgtaccgg gccgtcgtgg gcttgcgcat cgatacgcag tacaaccaga tacccgacga
    13321 cagcttcgca agcattcaga cgcaggggct gctcggtggg aagtacatcg gcttcagccc
    13381 cggcgggtcg gacacctttc tcaagaacgg cagccgagtc gagcagacgc agtcggcgat
    13441 cgtgctcgag agtctgatca acaagctgtt cgcgagcttc gcggacaaga gcggaagcgg
    13501 cggcgctaac agcaccagta acggtggcag tgcgaaaccg gaggagcaga agaaatgaga
    13561 cctgtgacga tcatcgtagc cggcgccctg tttgcggccg ccgggtccgg cgtgccggcc
    13621 gctgctgcgc ctgccccgcc tgccgctgat tcgccagcac ccaccccaca ggcgcaaggg
    13681 gcgcagtccc aggagagccc gagcgagctc gtccagcaga ccgcgcaagg gattctcaag
    13741 gacctggagg ccaatcgggc ggcctacaag caggatccat ccaagattgc ggccctcgtg
    13801 gacaagtacc tgttgccgca cttcgacacc gaatactcgg ccaagctggt cctggcccag
    13861 aactggcgaa cagccacccc ggaccagcgc aagcgcttca tcgacgcgtt ctaccattcg
    13921 ctgatcacca actacggcgc ctatctggcg gatttcacgg cggaccgcct caaggtgttc
    13981 ccatcgactg tcgagccggg cgccgatcat gcgaccgtac gtacggaagt cagacgtgac
    14041 aatggcgacc ggatcgccgt gaactacagc ctgcacatga cccctcaggg ctggaaggcc
    14101 tacgatgtca acatcgacgg gatcagctat gtgaagagct accgcgatga tttcggctcg
    14161 cagattcagc agcaggggct cgattcagtc atcagccggc tcgagcgcgg cgagaagccc
    14221 ggcgacatcg ccaagacggc gagtggcaag aaaagctgaa gcggtcagcc gcccacggca
    14281 cggggagtcg atgaaggcag ccaactcagc cgctccggac agcctcgagc ttcaggaggg
    14341 gagtgcaggc cagttcgtcg cgcggggacc gctgacgttt gccacagcgc gacgcagccg
    14401 cgaactcggg ttgtcggcct ttggcgctgc ccgctcgcgc gagatcctgg tggagtgcgc
    14461 gggtatcagc gcatccgaca gcgccggaat gaccgtgctg ctcgactggc tctcgtatgc
    14521 gaagcagtcc ggccgagcgc tgcatttcgg caacctgccc gctcaggttc aggcgatcgc
    14581 acgcatcagc gacgtcctgg aactgttgga gcggggcgtg tcctagtgct cggcgcagtc
    14641 gctttgggag gggctctgcc ctctcagtgc ggctgatccg gtggcggcgg ctggggcggc
    14701 acgggcgagg ggttgggttc gttgctgcgc ggcggagcgg gctccgtggg ctgacccggc
    14761 gtgccaggtt gtgggccctg cggcgaattt gctttgggcg gggtctgagg ttgctcgccg
    14821 ccttcagtct cctcgaggat cttcttctca tcctcgtact gctgctgctc ctgctgctgc
    14881 tgttgtttcg aggtctcccc gccggtgacc agatattgcc gcctctgcag atagacattg
    14941 cgcaggaacg catagcggtc ataggcccct tccaggacct tctcggagtc gagcagccgg
    15001 gagcgggtat cgactacctg cagcccccac agcccgtagg acacccacga gttgctgatg
    15061 taatggcgcg gatcggtgaa gatgtcgccg actctgccga tgccgtcgcg caggtcggag
    15121 ggcccgagga aggggatcac gatgtacggc ccgggcccgg cgccgtagtg tccgaaggtc
    15181 aggccgagat cctcctcgtt cttctgcagt ccgatcctcg tggccggatc gaacaagccc
    15241 ccgattccca ccgtggtgtt gacgaccagc cggcccgtgt cctgcaggaa gggcttgaat
    15301 ttcagctgca gcaggtcgtt cacgatggtc gtggtatagg acagattgtc gatgaagttg
    15361 gatacgccgg tgcgcaccgg ccggggtgtc accttgcgat agccgagggc gacaggcttg
    15421 agcactgcac ggtcgacggc atcgttgaaa cgataggttg cgcggttgac gcgctcgaag
    15481 ggatcgcgcg ggtcaggttg agtgcctggc gccgtggcgc acccgaacag gctcaggcag
    15541 agcatggcta gcagcggcag ggaggctgtt cggggcatgg cgtgggtgag acctgaaaag
    15601 ggagcgcgca ttgtaccagc gccaccggat cctgcagatc cgcggggctc aaggtttgtc
    15661 gcgggcggat ggcgcgcgag aacgctattg cgtgttgctg acgtgccgca ggcgggtgtt
    15721 ggcgaggtac tcccggacct catccaatcg cgcctggtaa cgcgcccgtt gcacctgtgt
    15781 cagatgtggc gctgcgagtg cgagttgaag ctgctgggcg gccaggggca gattgccgcc
    15841 tgcgatctgg tactcgccca tatagaaata ggcatccccc ggatcacccg cggcactggc
    15901 ggccatcgcg gtcagccgga tctgctcagg agtcggcggc acgttgttga acaagtcgag
    15961 gagcacgtta tgtgcctcgg ctgcgcgccc ggccttcatc aaggcctcgg catagcgcac
    16021 ggtcaccggc acgttgcgcg gaaagagcgc gacggcatgc tgaagagtcg caacgccatc
    16081 attggtctgg cccgctttga cctgcgcctg gccgagcgcc gcatgcagca atgtcagccc
    16141 ctcgtgctgc tcggtcatct tgtcgagaat cttctcggcc tccaccggcc ggttgtccgc
    16201 catcagcgcc agggcctcgc cgtagcgcgt gccgatgtcg ttgttgccct gtgcgatttt
    16261 ctgcgcgtac tgtttttcga tgtccacatc gccgggggtg gtcagcaccc gcaggcgctc
    16321 gcgaatcagc tggtaattca gcgactcctt gaccttgacc ggcggaagct gggctgcgcg
    16381 cgagcgcgcc tcggcaatac gcacggaggt gacgggatgg tcctgcagca tcgcagggat
    16441 gtaggttcca gcgagtccgt cgacacgccc gatagtctcg aagaagtttg ccatggcgcg
    16501 cgggtcgaag cccgcgcccg cgaggaagcc gataccaaca cggtcagctt ccgcctcgtt
    16561 gtcgcgcgtg aagttgatct gcgcctggac cgccatgccc tgggccgccg caatgccacc
    16621 ctcgatcgcc tggccaccac cgccactgag cgcaccgatc agcacggcag ccagcatggc
    16681 tgccgctgcc gccatgctct gctgaccctg tgcgcggatc gcccgcgcga tgtggtgttg
    16741 ggttacgtgc gccatctcgt gtgccatgac gctcgcgagc tcagactcgt tgtgcgtggc
    16801 gaggatcagg ccatagttga tgcagatgaa gccgccaggc agggcgaagg cgttgatcgc
    16861 cgactcgcgg aaggcgaaga actgaaagtg cggcgcaccg tcgggcgcct gcgtggcgag
    16921 tttgtccccg accgcattga ggtattccga aacctccggg tcttcgatga gcgcgttctg
    16981 ctcgcggaac tggtgaatga ccatggcccc gagctggtac tcctccgtgc cattcagcac
    17041 agcggccgcg gggctgccca tgtcgggcag gtcgggcggc gcaggggtca ccggctgctg
    17101 cgctgaggct gccgcggcga aggtcgtgaa agcggctgca aggcacagga tcagggcacg
    17161 catgattcgg attcgaccgt cagcaggtgc ggagagtttc cgcgggatta cagaagctta
    17221 caaagtgtcc gatgcaaagt cggccagccg ggagcgctca ccgcgcacca gggtaatgtg
    17281 ccccgagtgt gcccagccgc gaaagcgatt tacagcatat gtgagacccg aagtcatctc
    17341 cgtgaggtac ggcgtgtcaa tctgggcgat attgcccagg caggccacct tggtgccggg
    17401 accggcccgc gtgacgaggg ccttcatctg cttgggggtc agattctggg cctcatccag
    17461 gatgatgaag cggttcagga aggtgcgccc gcgcatgaag ttgagcgagc ggatcttgat
    17521 gcggttgcgc agcaggtcat tggtcgcagc ccggccccac gttccaccct cctggctgct
    17581 cgtcagcacc tccaggttgt ccatgagggc ccccatccag ggctccatct tttcctcctc
    17641 ggtgcccggc aggaagccga tatcctcacc cagggggatg gtgacgcgag tcatgatgat
    17701 ctcgacgaag cggttggtct cgagcgtctg catgagcccg gcagcgagcg tgagcagggt
    17761 cttgcccgta cccgcagggc cgaggacggt cacgaagtcg atctcgggat ccatcagcag
    17821 gttgagcgcg aagttctgct cacggttgcg cgcggtgatg ccccagacgc tgtggcgctc
    17881 ggtacggtag tcgcgcaaca gctcgatcgt ggcgggggtg ccgggctgaa ccctgcgcac
    17941 catggcctcg atcccctgcg gactgtcctc ataaagtccc tggttcggtt gccactcgct
    18001 cacagagggg cccgtgacct cgtagaaggt ccgctccgct tctttccacg agcgcatgtc
    18061 cttgccgtgc ttctcccaga aatccacggg cagggcgctc atgccgctgt agaggacgtc
    18121 cgcgtcctcg agcgtcttgt cgctgtagta atcctcggca tgcacgccca ggaccgccgc
    18181 cttgatgcgc agattgatgt ccttcgagac gaggatgacg cgggcatccg gtcgcccgtt
    18241 ctgcagcgcg agtgtctggg cgaggatcag gttatcgctg ccctggccgg gcagcccggc
    18301 aggcagtgcg ctcgagagct cgcgtgtctg aaagaacagc cggccggtcg aaggcttctt
    18361 gccatggttt ccggaatagc cgctcggcag ctgaagccct tggtcgatgt gctccttggt
    18421 ggcctcgcgc atcatgtcat cgaggaagcg gctcacctgg cgcacgttgc gggaagcctc
    18481 cgagagaccc ttcttgtgcg cgtcgagctc ctcgagcacg accatcggca ggtaaacatc
    18541 gtgctcctcg aacctgaaga tcgaagtcgg atcgtgcatg agcacgttgg tgtcgagcac
    18601 gaacaggcgc cgatccccgc gcgccttcgc cttcagctcc ccggcggcgg cggaggccgg
    18661 gccgggctca gagagacttc gccgcttcaa gcacttcctc cgcgtggccg gggaccttca
    18721 ccttgcgcca ttcgtgccgc agaacaccgg cgccgtccag caggaaagtg ctgcgctcga
    18781 tcccgaggta cttcctgccg tacagactct tttcgtggat gacatcgaat gccttgcaga
    18841 gcttttcctc ctcatcggcg agcagcggaa aaggcagctt ctccttgtca gtgaagcgat
    18901 cgtgggagcg gacgctgtcg cgcgagacgc ccaccaccat ggtgcctgcc ttgcggaatg
    18961 cggcgtgcag atcgcgaaag tcctggctct ccctggtgca gccggaggtc atgtccttcg
    19021 gatagaagta gagcaccagc ttcctgccgg ccgcgttctt cagtccccac gttccgccgc
    19081 cactggcgga gagggtgaaa tccttgactc gcgtgccgat cttcggcggg ggcatcttgg
    19141 cggctccttg aggcttcagg ccgcgcgcgg cctgaagccg cacggcgcat ccatccacta
    19201 cgattttacc ggctcgagaa tggcatcgag attgagcgag tcgcagaatt ccatgaattc
    19261 ctcccgcagg tgggcgacgt ggatgcggct cggcacgttc acgatcatct gcacggagaa
    19321 catcggtgcg ccggtgtgcg cggccggata gctgcgcgtg gaaacctcgg cgatgtcgat
    19381 tccacgggtc gcgaagaagc ccgacagccc cgagacgatc ccactctggt cgaggcatac
    19441 gacatcgatc gagtagggca gcatgtcggt gcgcgccgta cgcggctcgg tgcgcttgag
    19501 atgaatgctg agactgctcg tttccgccag gcgcttcagc tcggtttcga gcttggcgag
    19561 ggagtgccag ttgcctgaca ccagcagcag cattgcgaac tccgtaccga ggctcgccat
    19621 gcggctctcg ctgatgttgc cgccgcactc gctgatgacg cgcgtcagct cgtggaccat
    19681 cccagtacgg tcgccgccga tcgccgaaac ggccaggtgc tgcttcatct tgcgtgtcgg
    19741 ttggaagtcc gctgtcctcc ggcccgagtg taccgggtgc gcctcgccca gtcatgggct
    19801 gcgcccaaat tgccatgctt ccctgcgtta cgatgcattc tccggccgcg cgccgcaggc
    19861 cgcgagtcgc aagcgaggtc ctcgcctcgc cagcggcttg cttgaccgga gcgccgcctc
    19921 gtaacatccg tctcctcacg tcacgccggt gcgaggcgcc ggtgattttc acgatagcag
    19981 atcgacctat gttctcaggc agcctggtcg ccatggtgac gccgatgcgg ccggacggag
    20041 cgctggacct gcaggcatgg gagcggctca tcgagctgca tctatcgagc ggcacgcgcg
    20101 ggctggtggt cggagggacc accggagaat ccgcgacatt gagcgaggcg gagctgtggg
    20161 agctgaccga gcgggcgtgc gcccaggccc gtggtcacat tccggtcatc gtcggcgccg
    20221 gcacgagcag cacagccgcg accgtggagc gcgcgcgccg cctctgcgag ttgccgatcg
    20281 acgggttgct gctcgttacg cctgcgtaca atcgaccgac gcaggaggga ctttaccgac
    20341 atttcgctgc cgtcgcggct gcgacccggc gtccgctgat tctttacaac gtaccctcgc
    20401 ggacctcagt cgacatgctc cccgcgacgg tgcagcgact gtcgcaactt cccggcatcg
    20461 tcgcggtcaa agaggcggtc gcagacgtcg agcgcattcg ggagctggtg gcggtgtgtt
    20521 cgcacgactt cgcggtcttg agcggtgatg acgccactgc tcgatcatcg atactcgccg
    20581 gcgcgcgcgg cttgatttca gtgactgcaa acgtcgcgcc tcggcaggtc tcgtccatgg
    20641 cggctgcggc catgaaggga gatgccgcgg aggcgggccg cctggatgcc gaggtcgcag
    20701 cgttgcatcg ggagctgttt ctggaggcca atccgattcc cgtgaagtgg gcgctgaggc
    20761 gcatggagct gatacagagc gggatccgct tgccgctcac cgaactgtcc gagacgtacc
    20821 ggccggcagt gctggccgcg ttgcgtgccg cgggcatcct gaattgattc gacacaaagg
    20881 gccgaaatga ttcatcgcat cgggctggcc gcattgctgg cggccctggt catccttgca
    20941 ggctgccatc cgttacgctc gctgcgcaac gtgggcggct cctgtcacga cgttcagccc
    21001 tacaccaagg cgcagagcat tgcgccgttg catattccgg ccggactgga tgcgccggac
    21061 acgagcaact cgctgcaggt gcctgcgctc aacgagcccg caccgccgcc gatcggaccg
    21121 aaggatccct gtctagatcg gccgcctccg ttcaaagtgg cgaagccggc aacgcctcag
    21181 gcctgagggt gtatcattcg ccgccccgtg cggcgcgctc ggacggtccc attggccgcg
    21241 ctcggcggtg ccgcagcacg gactcgcggt gccaatattg tccgaggagt tcggcgcaag
    21301 catttgattg gagaggtggc cgagcggtcg aaggcgctgg actggaattc cagtaacatc
    21361 ttcacgggtg ttcgtgggtt cgaatcccac cctctccgcc agatgcacga gcgccgtcag
    21421 cacatcggag cccgcgcgag gcggtagaat tccaggtaga gcagtaaggc ggttcaagac
    21481 ctaattaaat cagtgtgttg cgggggaaag tcaatctact ttga
//
LOCUS       DASRHN010000163         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1702283, whole genome shotgun sequence.
ACCESSION   DASRHN010000163 DASRHN010000000
VERSION     DASRHN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702283"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="VFB37_06435"
     CDS             <1..1626
                     /locus_tag="VFB37_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO22127.1"
                     /translation="VFLDMRMPPGQDGVWAAARIRELDPAVEIVVCTAYSDADPCEIG
                     GIVPPEDKLSYLQKPFHPHEVRQMTIALGSKWRAERRIVKLAYFDTLTGLPNREQSRT
                     RLIGALDAAKEYGRNLAVLYLDLDNFKHVNDTLGHAVGDELLCQVANRLRESLRYGDS
                     IGAESAASARPGDLARLGGDEFMVMLPALRRREDAAAVAERLMHALREPMRIADNSLV
                     ITPSVGIAMYPQDGADAETLLRNADLAMYFAKRTSPGTFAFYDAEMNSSALHRFTIEE
                     RLRGALHRNELAVDYQPQFDLRTGGISGMEALLRWSSPELGVVPPAEFVPIAEETGLI
                     YPIGEWVLRTACRQAKAWLAEGLPLGRMAVNVSGRQFVLAGYPELVASVLRETALEPA
                     MLELEITESVVMANEHWAEHVLCQLKQIGVSLAIDDFGTGYSSFGRLRHFSVDRLKID
                     RSFVSSISQGTDDRVIAAAIIEMSRSLRINVTAEGVESLPQLTFLQEHECHDAQGHLL
                     SKALAAEDAQKLLRRAGELAEGSRTQRLKAIIG"
BASE COUNT          365 a          638 c          721 g          401 t
ORIGIN      
        1 gtatttctgg acatgcgcat gcctccaggg caggacggtg tgtgggcagc agcccgcata
       61 cgggagctcg atcccgctgt ggagatcgtg gtgtgcacgg cgtattccga tgctgacccg
      121 tgcgaaatcg gcggtatcgt acctccagaa gacaagctgt cgtatctgca aaaacccttc
      181 catccgcacg aagtgcggca gatgacgatt gcgctcggaa gcaagtggcg cgccgagcga
      241 cgcatcgtga agttggccta tttcgacacg ctgaccggcc tgcccaacag ggagcagtca
      301 cgcacgcgac tcatcggagc gctcgatgcg gcgaaggagt acggccgtaa tcttgccgtg
      361 ctgtatctgg acctcgataa cttcaagcac gtcaacgaca cgctcggcca tgccgtgggc
      421 gatgagcttc tctgtcaggt cgccaaccgg ctgcgcgaat cgttgcgcta tggggattcg
      481 atcggagcgg agtccgccgc cagtgctcgg cccggcgatc tcgcccggct cggcggcgat
      541 gagttcatgg tcatgctgcc agcgctgcgc cgtcgcgagg atgcggcggc agtggcggag
      601 cgactgatgc acgcgctccg cgagccgatg cggatcgcag acaactcgct cgtgatcacc
      661 cctagcgtgg ggatcgcgat gtacccgcag gatggagcag atgcggaaac gctgctgcgc
      721 aatgccgacc tggccatgta cttcgccaag cgcacgagcc caggcacttt cgcgttctat
      781 gatgctgaga tgaacagcag tgcgctgcac cggttcacga tcgaagagcg gctccgcggc
      841 gcactgcatc ggaatgagct cgcggtagac tatcagccac agttcgatct gcgcacgggt
      901 ggcatctccg gcatggaagc gctgctgcgc tggagcagcc cggagctggg cgtggtgccg
      961 ccggcggagt tcgtgccgat cgccgaggag acggggctga tctacccgat cggcgagtgg
     1021 gtgctacgca cggcctgtcg gcaggccaag gcatggctcg ccgagggact gccactcggc
     1081 cgaatggccg tcaacgtttc gggacggcaa ttcgtgcttg ccggatatcc tgagctggtc
     1141 gcctctgtgc tgcgcgagac cgcgctcgag cccgccatgc tcgagctgga aatcaccgag
     1201 tcggtagtga tggccaatga gcactgggcg gagcatgtgc tgtgtcagct caagcagatt
     1261 ggcgtttcac tcgccatcga tgacttcggc accggctact cgagcttcgg acgcctacgt
     1321 catttctcgg tggaccgtct caagatcgat cgctccttcg tcagcagcat cagccagggc
     1381 actgatgatc gggtaatcgc cgctgccatc atcgaaatgt cgcgctcgct gcgaatcaac
     1441 gtcaccgccg aaggcgtcga gagtctcccg cagctcacgt ttctgcagga gcacgagtgt
     1501 cacgatgcgc agggccatct gctcagcaag gcgctcgctg cagaggatgc gcagaagctt
     1561 ttgcggcgcg cgggtgaact ggcagagggg agccgcaccc agcggctgaa ggccatcatc
     1621 ggctgaggtg gccggcgcgc gggttggggt ggggacggtt tgcgcctgcg ggtctgttgc
     1681 ggctcctgaa ggtgttgtct ccgcgggatg ctttgggcat tctgcccaag tgatcgagaa
     1741 cagctcggcc cgacgggcgc gttacggcct ctaccttctc gggggcgcct tcgtcgcgct
     1801 cggattctca ctcgaaatgg tgcctgcgct ggctgccgtt gcgcctgcgc tcgcaggcag
     1861 agtctgcgct ggggtcggag ccgcgatcct gtcagtcggc cgcttcggat ccgaccggct
     1921 cgtgtgccgc tgcgaggcgc tgttccgagg ccgatactcg ggacgacccg gttagagtga
     1981 gcgcccgtcg aagtgcttga cgggcacgct caaggggtca attccctcaa gggagcgtag
     2041 gttgattgcc accgttggcg tgccatccgg agctgtggcg tcggcaaaag cgtgaatccc
     2101 acaggtcttg cagaagcggt gattg
//
LOCUS       DASRHN010000164         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1869058, whole genome shotgun sequence.
ACCESSION   DASRHN010000164 DASRHN010000000
VERSION     DASRHN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869058"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VFB37_06440"
     CDS             complement(<1..1161)
                     /locus_tag="VFB37_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZO22128.1"
                     /translation="MHSAVEVLLDHLGRLRLWQKLALLVCAMLIPAVLLGCFYFGSTS
                     AAIGRAHEELEGARYLKAVGALSAEILTHRDRAFAVLVGDKARQPELLAQTQEVDTRI
                     AAVDVIDAELGTRLQASAAWQSVKAEWAALKTKTLQHTADEDDAAHLVLAAHLDQLAE
                     AIGVRAMTSFDPESETRTLVRVASDYAPALLSAAGEVRRHAVRAAAKGYLGGDDRMGI
                     RLFHDRQSQLLSSLEGALEQLPSAARKILQPQLQSSRSACDAFNAVIQSRILGAPNIE
                     AGAGEIYDSGTAAVRELKSTSLMAYDLLNTRLAERLSRLRERQMFSAAITGPALGLAL
                     LLAWLMVRSLSRRLRQAVDAFARIADGEYDNSLDTRGRDEASQVLRALQQMQS"
     gene            complement(1186..1716)
                     /locus_tag="VFB37_06445"
     CDS             complement(1186..1716)
                     /locus_tag="VFB37_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZO22129.1"
                     /translation="MSSNTTEQMAKAAGAEFNQVLTFVLGSETYGLDILRVQEIRGWS
                     AVTKIPHAPAHVLGVLNLRGSIVPIVDLRMRFALERAEYTAVTVIIVVSVVSAAGRRD
                     FGVVVDGVSDVVNVNPAEVKAAPELGARGATDYIRGLVPSADRMIVLLDIDRLIGGDL
                     TALSSSLAPAAAASAA"
     gene            complement(1741..3891)
                     /locus_tag="VFB37_06450"
     CDS             complement(1741..3891)
                     /locus_tag="VFB37_06450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZO22130.1"
                     /translation="MTIDLAQFHDAFFEESFEAIDSMEAALLKLDVGSPDKELINTIF
                     RVAHSVKGGSATFGFSEIASFTHSLETMLDELRGGAMQVSPSISDLLLKSVDIMRAML
                     RAVQLKQPIDAQRVSDLQFDLELTIAQNKAAAASNPTPLPAASVASAASAQKVAAAPQ
                     AHWRIEFRPYTELFARGNDPLRMLRELARLGELEVQAELEALPSFEHLDPQSCYLGWL
                     LELRGDASESAIRQVFEWTEGDCDLQIERVLSASADNSAVQGAAASASASAAASSVGP
                     AGGSDASAESSSALLAATSRGAPAAPGPTAPVTKDETKIELPMPTAPDSGSIRVSVEK
                     IDELMNTVGELVITQAMLSQLSSELEGTVAEKLRGGLAQLERNMRELQESVMRVRMLP
                     INTVFSRFPRMVRDLAQRLGKQIELKMTGEQTELDKTVLEKIGDPLVHLIRNSIDHGI
                     ESPAARAAAGKPPAGTVHLDACHRGGNIAVEVSDDGAGLDKERILVKARDRGLIGADD
                     TLSDEQVYDLIFMPGFSTADKTTDVSGRGVGMDVVRRNVKELGGKIELRSQRGKGSKC
                     VITLPLTLAIVDGQSVAVGAETYIVPLVSIVESLQLRSSSVSRLSGQGEVFSFRGYYL
                     PIVRLYEVFGVEPRTRVLDEGLVVVAEGEGHQRVGLFVDDLLGQQQVVIKSLETNYGH
                     IEGVSGATILGDGSVALILDVPGLIRALSARAAA"
     gene            complement(3888..4361)
                     /locus_tag="VFB37_06455"
     CDS             complement(3888..4361)
                     /locus_tag="VFB37_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZO22131.1"
                     /translation="MKKRRKTASQPVAHPRQAARHGQAARNGQAKRKGQTGRKAAASA
                     TPRRIRTERRSAIPEHPRAPPAPNSAFTLAGDCTISETTALKEGLGRLLAEARPVTLE
                     IAGLERVDTAAMQLIAAFVQHRERQGLAIEWHGSSPALSSAARLLGLAGLLKLPA"
     gene            complement(4481..4897)
                     /locus_tag="VFB37_06460"
     CDS             complement(4481..4897)
                     /locus_tag="VFB37_06460"
                     /inference="COORDINATES: protein motif:HMM:NF022428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HZO22132.1"
                     /translation="MSVPSVESMLVAGRAVFLVFSFIVAAVTFSRWRRAALAQTERLL
                     AQHDLLLKRFSELESHLEGARAALAELGERLDRPRHVATPAGSASQGYEIAVRLARGG
                     ATREELISSCGLSQHEAELVWRLHAPRNALARLKTA"
     gene            complement(5029..6129)
                     /gene="motD"
                     /locus_tag="VFB37_06465"
     CDS             complement(5029..6129)
                     /gene="motD"
                     /locus_tag="VFB37_06465"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HZO22133.1"
                     /translation="MRHTTARRRRAHEEHTNHEAWAIPYADLLTLLLAFFVVMYAISS
                     VNAGKYRVVSDSLFAAFHGSPRAATPIQVGDKPVDSGADAQAGVVQQSTVKGPGPSQL
                     APVPVEVGLSRSRGDVTLPGKLPPQAAAAAAALSQLADQVERAMSDLVQKDIVTVRRN
                     DFWIEVEMRTDILFPSGSARLSDPAVGIIERLGSVLSPFPNPIRVEGHTDNKPIRTAV
                     FSSNWELSAARAGSVVRVLANHGVAPDRLAVIGYGEQRPAKPNTTSEGRNANRRVVVV
                     ILSTELMRQTDPAKLTPPTAAAHGAASVPQANSVPAPNALGLPVAQPGEPATPARGGA
                     IASPGDTPQAVPVPTSATAIKPQHNSGTEPAP"
     gene            complement(6135..6875)
                     /locus_tag="VFB37_06470"
     CDS             complement(6135..6875)
                     /locus_tag="VFB37_06470"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HZO22134.1"
                     /translation="MDILSVIGLVLALNAVLVGAVLKGAGLMALVSAAAFMIVVVGTL
                     AAIFIQTPLPVMRRALRMFPWIMRPPTRDGQLLIAKIVEWSNIARRQGLLGLEIMIDR
                     EPDDFVRKALQLVVDGTEPDAISSVMYVDLNMREHADTTAAKVFEGMGVYSPTLGIIG
                     AVLGLMAVLQNLADPSKLGHGIAAAFTATVYGIGLANLFFLPVAAKLKVLVQRQTQLR
                     EMIVDGMLCIAQGENPRAIEAKLQGYLH"
     gene            complement(6885..>7110)
                     /locus_tag="VFB37_06475"
     CDS             complement(6885..>7110)
                     /locus_tag="VFB37_06475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HZO22135.1"
                     /translation="QDLTGQIIRSVMKLVAELEGALASLARLSGDLVEHATMGQTEGA
                     GQGPAIPGVTKGEIASGQTDVDALLSDLGM"
BASE COUNT         1203 a         2339 c         2371 g         1197 t
ORIGIN      
        1 gctttgcatc tgctgcagcg cgcgcagcac ctggctggcc tcatcccggc cgcgcgtgtc
       61 gagtgagttg tcgtactcgc cgtcggcgat cctggcgaag gcatcgactg cctggcgcag
      121 acgccgcgac agggagcgga ccatgagcca cgccagcagc agcgcgaggc cgagcgcggg
      181 accggtgatc gcggcagaga acatctgtcg ctcgcgcagg cgcgacaggc gttccgcgag
      241 cctggtgttg agcaggtcgt acgccatcag ggaagtgctc ttcagctcgc gcaccgcggc
      301 cgtaccggag tcgtagatct ctccggcgcc ggcttcgatg ttgggggcgc cgaggatcct
      361 cgactggatg acggcattga aggcatcgca ggccgacctg ctggattgca gctgcggctg
      421 cagaattttc cgggccgccg atggcaactg ctcgagcgct ccttcgagcg aggacagcag
      481 ctgcgactgt cgatcgtgga acagccggat acccatgcgg tcatcgccgc cgagataacc
      541 tttggctgcc gcccgcactg cgtgccggcg cacctcgccg gcggcgctca gcagcgccgg
      601 cgcgtagtcc gaagccaccc ggaccagtgt ccgggtctcc gactcgggat cgaatgaggt
      661 catcgcgcgg acgccgatcg cttccgcgag ctggtccagg tgtgctgcca gaacgaggtg
      721 cgccgcatcg tcctcgtcgg cagtgtgctg gagcgttttc gtcttgagcg cggcccattc
      781 ggccttcacg gattgccacg cggcagaagc ctgcagacgg gtccccagtt ccgcgtcgat
      841 cacatccacc gccgcgatgc gggtgtcgac ctcctgagtc tgcgcgagca actcgggctg
      901 gcgggccttg tcgcctacca ggacggcgaa ggcgcgatcc cgatgcgtga ggatctcggc
      961 agagagcgcg cccacagctt tgagatagcg ggctccctcg agctcctcgt gggcccgtcc
     1021 gatcgcagcc gacgtcgatc cgaaatagaa gcagcccagc agcactgccg gaatgagcat
     1081 ggcgcagacg agcagcgcga gcttctgcca cagtcgcagg cgccccagat gatcgagcag
     1141 gacttcaact gctgaatgca ctgccgtgtc cgttgcgcct gcaggctagg ccgccgaagc
     1201 cgccgcggcc ggagcgaggc tcgaagagag cgcagtcagg tcgccgccga tcaatcggtc
     1261 gatatcgagc agcacgatca tccgatcggc gctcggtacg aggccccgga tgtagtcggt
     1321 cgcgcctcgg gcgccgagct ccggagccgc cttcacctcg gcggggttga cattcaccac
     1381 atccgagacg ccatcgacca cgacgccgaa gtcgcgccgg ccggctgcgg aaaccaccga
     1441 aaccacgatg atcacggtca cggccgtgta ctcggcccgc tcgagcgcaa agcgcatgcg
     1501 caggtccacg atcggcacga tcgagccgcg cagattgagc actccgagca cgtgcgcggg
     1561 cgcatgcgga atcttcgtga cggcggacca gccacggatt tcctgcactc gcaggatatc
     1621 cagtccatag gtctcgctgc cgagcacgaa ggtcaggacc tggttgaatt ccgcaccagc
     1681 ggccttggcc atctgctccg tggtgttgga cgacatggct atctcctgtt ggttggatcg
     1741 tcaggccgct gctcgggcgc tcagcgcgcg gatcaggccc ggtacgtcca ggatcagggc
     1801 cacggagccg tcaccgagga tcgtggctcc ggatacgccc tctatgtgcc cgtagttggt
     1861 ctcgagcgac ttgatgacga cctgctgctg gccgagcagg tcgtccacga acaatccgac
     1921 acgctgatgg ccttcgccct cggcgacgac caccagaccc tcatcgagca cgcgcgtgcg
     1981 aggctccacg ccgaacacct cgtacaggcg cacgatcggc aggtaatagc cgcggaagga
     2041 gaacacctcg ccctggccgg acagccggct gacgctgctg gatctgagct gcagcgactc
     2101 gacgatggag accaatggaa cgatgtaggt ctcggcaccg accgcgaccg actggccatc
     2161 gacgatggcg agggtcaggg gcagcgtgat gacgcacttg gagcccttgc cgcgctgact
     2221 gcgcagctcg atctttccac cgagctcctt gacgttgcgc cgcacgacat ccatgcccac
     2281 gccgcgtcct gatacatccg tcgtcttgtc ggcggtggag aagcccggca tgaagatcag
     2341 gtcgtacacc tgctcgtcac tgagggtatc gtccgcgccg atcaggccac gatcgcgcgc
     2401 cttgacgaga atccgctcct tgtcgaggcc ggccccatca tcgctgacct cgacggcaat
     2461 gttgccgccg cggtggcagg catcgaggtg cacggtgccc gccggaggct tgccggctgc
     2521 cgctcgggcc gctggggatt cgatgccgtg atcgatactg ttgcggatca ggtgtacaag
     2581 ggggtcaccg atcttctcga gcaccgtctt gtcgagctcg gtctgctcgc cggtcatctt
     2641 cagctcgatc tgcttgccca gccgctgcgc caggtcgcgc accatgcgtg ggaagcggct
     2701 gaacacggta ttgatcggaa gcatgcgcac gcgcatgacg ctttcctgca gctcgcgcat
     2761 gttgcgctcg agttgcgcca gcccgccgcg cagcttctcg gcaacggtac cttcgagctc
     2821 cgagctcagc tgactgagca ttgcctgggt gatgaccagc tcaccgacgg tgttcatgag
     2881 ctcgtcgatc ttctcgacgc tcacgcggat ggagccggag tccggcgcag tcggcatcgg
     2941 cagctcgatc ttcgtttcgt cctttgtgac cggcgcggtg gggcccgggg cggccggcgc
     3001 gccgcgcgag gtggccgcca gaagtgcgga gctggattca gctgaagcat cggagccgcc
     3061 cgcaggccct acggatgacg cggcggctga agcagaagcg ctggcagcag ccccctgcac
     3121 agcggagttg tccgcgctag cggacaacac gcgctcgatc tgcagatcgc aatccccctc
     3181 ggtccactcg aagacctgac ggatcgcact ctcgctcgca tcgccgcgca gctcgagcag
     3241 ccagcccagg tagcacgatt gcggatcgag atgttcgaat gagggcaagg cctccagttc
     3301 agcctggact tcgagctcgc cgagccgggc gagctcacgc aacatgcgca gcggatcatt
     3361 gccgcgcgca aataattcgg tgtaagggcg gaattcgatg cgccagtgcg cttgcggagc
     3421 ggccgctacc ttctgtgcgg aggcagcgga cgccacggag gcagcgggga gcggtgtcgg
     3481 gttcgaagcg gcggctgcct tgttctgcgc aatcgtcagt tccagatcga actgcagatc
     3541 cgagacccgc tgcgcgtcga taggctgctt cagctgcacg gctcgcagca tcgcgcgcat
     3601 gatatccacc gacttcagca gcaggtcgga gatggacggc gacacctgca tggccccgcc
     3661 acgcagctca tcgagcatcg tctccaggct gtgcgtgaag gaagcaattt cgctgaagcc
     3721 gaaagtcgca ctgccgccct tgacggagtg ggcgacgcga aagatggtgt tgatcagctc
     3781 cttgtcgggc gagccgacat cgagcttcag cagcgcggcc tccatggagt cgatcgcctc
     3841 gaagctctct tcgaagaagg catcgtgaaa ctgtgcgaga tcgatcgtca tgccggtagc
     3901 ttgagcaggc ctgcgaggcc gagcagccgc gccgcggagc tgagggcagg ggagctgcca
     3961 tgccattcga tggccagtcc ctgacgctca cggtgttgca cgaatgccgc gatcaactgc
     4021 atggcggccg tgtcaacacg ctcgaggccg gcgatttcga gtgtcacggg acgcgcctcc
     4081 gcgagcagac ggccaagacc ttccttgagc gccgtggttt ccgagatcgt gcaatccccc
     4141 gccagggtaa aagcgctgtt cggggcgggt ggcgccctcg ggtgctcagg gatagcggat
     4201 ctgcgctcag tcctgattcg gcgtggcgtc gccgaagcgg cggccttgcg ccccgtctga
     4261 cccttgcgct tggcctgccc attgcgcgca gcctgcccat ggcgggcggc ctgccggggg
     4321 tgggcgaccg gctgcgaggc ggttttcctg cgctttttca tctctgcggc ggctgtaggc
     4381 gaaaatgcgg caacgccgtc ctatcggcac gaaagccacg atgcttgagc ctgtttcggc
     4441 gaaagcggac gcagttccac cagccccccg gcacgcgagc tcatgcggtc ttcagcctcg
     4501 cgagcgcatt gcgcggggcg tgcagccgcc atacgagctc tgcctcgtgc tgcgagaggc
     4561 cacaactgga gatcagctcc tcccgagtcg caccgccccg agcgaggcgg acggcgatct
     4621 cgtagccctg cgaggccgag ccagcggggg tcgccacgtg ccgcggccga tcgagacgct
     4681 cgcccagctc ggccagggcc gctcgggccc cctcgagatg ggattcgagc tcgctgaagc
     4741 gcttcagcag cagatcgtgc tgcgccagga gacgctcagt ctgcgcgagg gccgcgcgcc
     4801 gccatcggga aaatgtgacc gccgccacga tgaaggagaa aaccaggaac acagcgcgtc
     4861 cggcgacgag catcgattca acagaaggca cgctcatggg cgttgatctc cttaccgggg
     4921 gcgaaaaggc gtggcttgcg tccgctcagc gtgagcgagt tcacacttgc ccaggggtcc
     4981 agccgcgccg catgtcgggt ttccggcgct gcgcgccgaa ctcctcactc agggtgcagg
     5041 ttccgtgcca ctgttatgtt ggggtttgat cgcggtcgcg gaagtgggaa cggggaccgc
     5101 ttggggcgtg tcacccgggg aggcgatcgc gccgccgcga gcgggagtcg caggctcgcc
     5161 cggctgggcg acaggcaacc ccagagcgtt cggcgcgggg acactgttgg cctgcggaac
     5221 gctcgcggca ccgtgggctg cggccgtcgg cggagtgagc ttcgccgggt ccgtctggcg
     5281 catcagctcg gtagacagga tcacgacgac cacgcgacga ttcgcattac ggccctcgga
     5341 tgtcgtgttg ggtttggctg gccgctgctc accgtagccg atcaccgcga ggcggtccgg
     5401 agcgactcca tgattcgcga gcacgcgtac gacgctgccc gcgcgagcgg ccgagagctc
     5461 ccagttggag ctgaacaccg ccgtcctgat gggcttgtta tccgtatgtc cttcgacgcg
     5521 tatcgggttg ggaaagggcg aaagcacgct gcccagccgc tcgatgatgc cgacagccgg
     5581 atcggacagc cgcgcgctgc cgctcggaaa caggatatcc gtgcgcatct cgacttcgat
     5641 ccagaagtca ttgcggcgca cggtcacgat gtccttctgc accaggtcgc tcattgcgcg
     5701 ctcgacctgg tcggcgagct gagagagcgc tgcggcggcg gcggccgcct ggggtggcag
     5761 ctttcccggc agggtcacat caccgcgact gcgtgacagg ccgacctcga cgggcaccgg
     5821 cgcaagctgc gacgggcccg gtcctttgac agtggactgc tgcacgacac cggcctgcgc
     5881 gtcggcaccg ctgtcgactg gcttgtcacc cacctggatg ggcgtggcgg ctcgcgggct
     5941 gccatgaaat gcggcgaaca gggaatccga gaccacgcga tacttgccgg cattcacgga
     6001 agaaatcgcg tacatcacta cgaagaacgc cagcagcagc gtcaggagat ccgcataggg
     6061 gatggcccac gcctcgtggt tggtgtgctc ctcgtgcgcc ctgcgtcggc gtgcggtcgt
     6121 atggcgcatc tgtgtcaatg caggtagccc tgcagcttcg cctcgatcgc ccgcgggttc
     6181 tcgccctgcg caatgcagag catgccatcc acgatcattt cgcgaagctg cgtctggcgc
     6241 tgtaccagca ccttgagttt cgctgcgacc ggcaggaaga acagattggc gagcccgatg
     6301 ccgtagaccg tcgcggtgaa ggcggcggcg attccatggc cgagcttgct cggatccgcg
     6361 agattctgca gcacggccat gagcccgaga accgcgccga tgataccgag cgtcggcgag
     6421 tagacgccca tgccctcgaa caccttggca gccgtcgtgt cggcgtgctc gcgcatgttg
     6481 agatcgacgt acatgacgct gctgatcgca tccggctcgg tcccgtccac gaccagctgc
     6541 agcgccttgc gcacgaaatc atcgggctcg cggtcgatca tgatctcgag ccccagcagg
     6601 ccctgccgcc gggcgatgtt gctccactcg acgatcttcg cgatgagcag ctgaccgtcc
     6661 cgggttggcg gccgcatgat ccaggggaac atcctcagtg cgcgtcgcat gaccggcagc
     6721 ggcgtctgga tgaaaatcgc cgccagcgtg ccgaccacga cgatcatgaa ggcggcggcg
     6781 gaaacgagcg ccatcaggcc cgcgcctttg agcaccgcgc ctaccagcac ggcgttcagc
     6841 gcgagcacca gtccgatgac gctcaggata tccaacgcga tgccttacat tcccagatcc
     6901 gacaggagtg catccacgtc ggtctggccg gaggcgatct cacccttggt cacgccggga
     6961 atcgccgggc cctggcccgc gccctcggtc tgacccatcg tggcgtgctc caccaggtcg
     7021 cccgagaggc gtgccaggct ggcgagcgcc ccttcgagct cggcgaccag cttcatgacg
     7081 ctgcgaatga tctgaccggt gaggtcctgg
//
LOCUS       DASRHN010000165        12404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_546378, whole genome shotgun sequence.
ACCESSION   DASRHN010000165 DASRHN010000000
VERSION     DASRHN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546378"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VFB37_06480"
     CDS             <1..1219
                     /locus_tag="VFB37_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HZO22136.1"
                     /translation="AVPIYQTVAYSFDSAEHGAALFNLEAEGYRYSRISNPTNDVLER
                     RVADLEGGMEALSVSTGQAALHFAALTVAESGRNIVSVPQLYGTTYTLFGHILPKLGV
                     QGRFAKSDRPQDLAALIDEHTSAVFCETVGNPAGNVCDLEGIAEVAHRAGVPLIVDNT
                     VPTPILIRPIDYGADVVVHSLTKFLGGHGTTLGGIIVDSGRFPWEKHAARFPMLTEPD
                     ESYHGLVYTQHYGPTAYIGRCRSVYQRTMGAVLPPLSAFLLLQGIETVALRVERHVEN
                     AKRVAEFLRDDRRVASVNYAGFPESPYYPLVQKYLAGRACSLMTFAIRGGFDAGTRFY
                     DALRLFKRLVNLGDAKSLACHPASTTHRQMSPQEQRLAGIGPETIRLSIGIEHVDDIV
                     ADLDQALAAAVAD"
     gene            1237..2637
                     /locus_tag="VFB37_06485"
     CDS             1237..2637
                     /locus_tag="VFB37_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZO22137.1"
                     /translation="MPEPVPALFPSSEVRQRVEDELTRALLSAQARLLAGPVMPDLDM
                     ARFRDELRRFDFEAPQPLDTLLGWAITQLEHGAVHMGHPRYFGLFNPVANFPSQCADR
                     ISGAFNPQLASSGSSPAPVEIEAHVIRSIALRAGLPAQAGGHFTTSGSEANYTALVCA
                     LTRAAPHYGMQGVRAFTGPVAMYTSRECQPAWFKIAHHSGIGRASLKLIATDGQGRMD
                     CKALAAAIRADREQGVVPVMISATAGTTGGGMVDPLKDCAEIAREHGLWYHIDAAWGG
                     AALCSQRLRRLLAGVELADSLTIDAHKWFATTMGCGMFITRAPAVLSESFRVATDFMP
                     SSVPSVDPYLNTLQWSRRFMGLRLFLSLAAAGWSGYAQHIERAVEVIAQARNRLRSLG
                     WSVLNDSELAVLCVLPPAGFPPVRELVRKVLGSGRAWVAASTFEGREVVRICNTHGET
                     SLADVEELVAALQATR"
     gene            complement(2634..2894)
                     /locus_tag="VFB37_06490"
     CDS             complement(2634..2894)
                     /locus_tag="VFB37_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZO22138.1"
                     /translation="METDEGRTTRERLIGLVRQILGSPAANRPLPIDARLSDVGMSSI
                     KMVNLMLAVEGEFSIAIPQSDITPENFHSIASVEALVTRLIG"
     gene            complement(2906..3916)
                     /locus_tag="VFB37_06495"
     CDS             complement(2906..3916)
                     /locus_tag="VFB37_06495"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF003776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HZO22139.1"
                     /translation="MPLTIQAPALLGAADRRKAKRLRTSAALTVGLVNNMPDSALEAT
                     EAQFSRLLQSAAGPLPVQLRLCYLPEVARSPQTLDHLLRNYWPIDALLKEPLDGLIVT
                     GMEPGPGALNEEPYWKRFGQLIEWAQAHTVSSVWSCLAAHAAVLKLDGIARQRLEHKR
                     FGVFEHALQPGHPLVAEINAPLCIPHSRWNELPVAALRTAGYTILSSSPESGADLFVR
                     HGRSLLVFFQGHPEYDETSLLKEYRRDVGRFLRGQQASYPSLPRGYFNDQGVALLEEF
                     RSRALADAGQDPMASFPEAAVAQCLNASWRDSAAQIYRNWLAVLAAAREPVRSPAVAE
                     AS"
     gene            4046..5242
                     /locus_tag="VFB37_06500"
     CDS             4046..5242
                     /locus_tag="VFB37_06500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO22140.1"
                     /translation="MTIKAVPRPMEPGPADIPTRAQTVASIAAANAGSVDSAARFPAE
                     AFRAVKEQRLLGIFVPKALGGEGASIAEVADVCYRLGQSCSSTAMIYAMHQVKVGCLV
                     RHLDGSAALDRLVRQLCSEQLLLASSTTEGQGGGNVRSSEAPIEHQDGRITLERKASV
                     ISYGAYADGVVTTARRAADAESSDQVLVAFLKSDYTLTRLQGWNTLGMRGTCSEGYTL
                     KASGTPDQILPVPYATIHPQTMVPFAHLLWGSVWAGIAAGATARAQAFVRQAVRGSGG
                     QMPPGAPQFTKALTTLRTLRGMLASSLAAYQRVMHDPVALGSLEFQSMITITKVEASE
                     LACSTVMSALRACGLSGYRCDSEFSIERHLRDVLSSPIMINNDRILSNLASTALMTPI
                     AASVRD"
     gene            5270..6181
                     /locus_tag="VFB37_06505"
     CDS             5270..6181
                     /locus_tag="VFB37_06505"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: protein motif:HMM:NF005479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HZO22141.1"
                     /translation="MNDHSRVPDPIEHIGTALFSPLGVEGVYARTALYEDIVERLAGL
                     ISRERDPSAEVLRFPPVMSREQLEKSGYLKSFPNLLGCVCALHGSEAQIFAAAQSHES
                     GGDWTKSTQVIDLVLSPAACYPLYPIVAKRGAVPRGGLLFDVAADCFRHEPSRALSRL
                     QTFRMREFVRIGSPEEITVFRDQWMKRAEALAGELGLPYTMDVASDPFFGRVGQVMAL
                     SQRQQSLKFELLIPYYPGATRTACMSFNYHREHFGTVWGLADEQGELAHTGCVAFGMD
                     RLAVALFCIHGLDVARWPAAVRRELAF"
     gene            6201..7094
                     /locus_tag="VFB37_06510"
     CDS             6201..7094
                     /locus_tag="VFB37_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO22142.1"
                     /translation="MGLDPQIKRFLDRLAAMNPPSALALSVSQRRSAIESLLSLGGEK
                     GPVAHVEERLLPIPGGRVGIRIYTPMDPEPLPAAFVFFHGGGLVAGSLDTHDGICRSL
                     ARAGRCRVISVDYPLAPEHPFPAALSAGYAATQWIAEHPQLLQIDPHRLIIGGDSAGA
                     TLAAVICQMATTRKEVRFALQVLLCPIVDFSASTPSRCEFAQGYLVDEATLAHDVKHY
                     LGPDALATDPRVSPGRAADVADLPPTCIHTAEFDPLRDEGKAYAERLQQAGVKTTYRC
                     HSGMIHLFYGMGALIPSVARA"
     gene            7157..7540
                     /locus_tag="VFB37_06515"
     CDS             7157..7540
                     /locus_tag="VFB37_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO22143.1"
                     /translation="MTSRSEVEQLLRALYATRIAGRRDELCALFAEQSRFRIGGTSDG
                     KPISVSANGAAEIRTWLTMFVKTFKISEHEILSMVIEGERAAVHWRANIHSRITGTLV
                     PTELVDLIEVRSAHITSYVEFFLPV"
     gene            7710..9608
                     /locus_tag="VFB37_06520"
     CDS             7710..9608
                     /locus_tag="VFB37_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO22144.1"
                     /translation="MSFPLSPDRASSLGEALYFDSGEHRLFGWLHRAAAEQGAKLGLV
                     ICKPFGYEAICSHRGVRLFAESAAALGVPTLRFDYAGAGDSCDIQPTDDQIEIWTQDV
                     LAAVQELQRQTGVQQVGLLGFRLGALLAILAARKSTAIRALALVAPVISGRRYIRELR
                     TTRLAAALALDPGNAAKPPESEAGSMEVSGFSLSAASLASLGAMDLATIDAPAISAVL
                     VIDRSDLPQCAQFAEKLSGSGIRTEYHSMAGLVGMLMTDPEFAATPELTVSVMGRWLE
                     RQRDGAGALSGATAQSPVSSSPQMPSLGLPGEGEAALSEHPVFFGSPATMFGIITEPA
                     PGEMRRRAVILLNVGAEHHIGSSRIHVSLARRWARHGYTVLRLDLTGLGDSETRPDRP
                     TNEVFPATAFEDIRIAMEVLRSRYGISDITLGGLCSGAYHSLRAAIMGLPVNRVLVVN
                     PMNFFWKEGMNAETLQQDVDVARNLGFYRDRLLSSVIWKRIFSGELGIGRLVRICLKR
                     PLLALESFFRGAARTFRIRLPNDLGWELEEIAARGVRTVFVFSRGEPGIDRLKMQAGS
                     TVKRLGDLCRVHIINSADHIFSQAAQRTTLESILTDELFARNQRANPTLARGLEIQPA
                     GNSLPGKH"
     gene            9679..10455
                     /locus_tag="VFB37_06525"
     CDS             9679..10455
                     /locus_tag="VFB37_06525"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZO22145.1"
                     /translation="MSELAKSAHESYLRAQPYPHIVFDNFFNPELLDIVLEEFPKPGA
                     IRWQKFDNAQEIKLASAAEASFGAVTRLLMYHLNSITFLEFLSAVTGIENLIPDPQFE
                     GGGLHQIVPGGKLGVHADFNKHRVFGLDRRLNVLIYLNKNWREDYGGYLELWGRDMTR
                     CEARVLPLFNRMMIFGTTDFTYHGHPDPLKCPEGMTRKSLALYYFTNGRPAEEISGEH
                     STEFRARKADDFVPTFRQRLRGVARELLPPIISRRLRGVL"
     gene            10471..11484
                     /locus_tag="VFB37_06530"
     CDS             10471..11484
                     /locus_tag="VFB37_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO22146.1"
                     /translation="MELGLVVIGRNEGERLMRCLASVREIPRKVYVDSGSTDGSVERA
                     GAQGVTVVELPVPPNFTAARARNSGLAMLLTQNPELEFVQMVDGDCEIRAGWVEEGLA
                     VLRADPKVATVFGRLRERFPDLSVYNALCDDEWNIPIGDATGSGGVALFRVSALRQVD
                     FYNEAMIAGEDSELSMRLRKRGWRLRRIDVEMAVHDAALTRFSQWWTRTRRSGHGFGE
                     MAFLHPDARSPNWPRSVRSIVFWGGAMPIVLAIACVLGVLFGGSWWALAALVLLVWPA
                     RMTQLAIRQWRRGLSIKVACASGVLLMLGKIPQLIGLAGFHRDRLTGRASSLIEHKRP
                     QRA"
     gene            11585..>12404
                     /locus_tag="VFB37_06535"
     CDS             11585..>12404
                     /locus_tag="VFB37_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZO22147.1"
                     /translation="MTAPKKVGLLGAGYILDSHAKSLAALPGVALHAVCDVSRGRAAQ
                     AAARFSIPHVFSSITELAGSDCDVVHILLPPGLHIEAASTLVEAGKSVFLEKPMGLDS
                     DACEALSRRAHERGLALGVNHNFLFTPGYEALRSSLKAGELGRLDHIALNWHFALPIL
                     QFGPFDSWMLAAPANVLFEIGAHLCAFVVDLVGVPQIDSAVAGNPISLPANQTVYRQW
                     TARAHAGSATVLLSISLTAGQADRILRVRGRGGSAQLDFGRDFGWCDLTVTDNPI"
BASE COUNT         2131 a         3899 c         3980 g         2394 t
ORIGIN      
        1 cgcggtgccg atctatcaga cggtcgccta ctcgttcgac agtgccgaac acggggccgc
       61 gctgttcaac ctcgaggccg agggctaccg ctactcgcgc atttccaatc ccacgaatga
      121 cgtgctggaa cgccgcgtcg ccgatctcga gggcgggatg gaggcgctgt cggtgagcac
      181 cggtcaagcc gccttgcact tcgctgcact gaccgtggcc gagtccggac gaaacatcgt
      241 ctccgtgccg cagctttatg ggaccactta caccttgttt ggccacattc ttcccaagct
      301 cggggtgcag gggcgctttg ccaaatccga ccgaccgcag gatctcgccg ccctgatcga
      361 tgagcatacg agcgccgtgt tctgcgagac ggtcggcaat cccgccggca atgtgtgcga
      421 cctcgaaggg attgcagagg tcgcgcatcg cgctggggtg ccgttgatcg tggacaacac
      481 ggtaccgact ccgattctca tccgtcctat cgactacggc gcggatgtcg tcgtgcactc
      541 cctgacgaag tttctggggg ggcacgggac caccctcggc ggcatcatcg tggacagcgg
      601 gcgctttccg tgggaaaagc acgccgcccg gttcccgatg ttgaccgagc cggacgagtc
      661 ctatcatggg cttgtgtata cccaacatta cggtcccacc gcctacatcg gccgctgtcg
      721 cagcgtgtat cagcgaacca tgggagcggt attgccgcct ctttctgcgt ttctgctgct
      781 tcaaggtatc gagaccgtgg ccctgcgtgt tgagcgacat gttgaaaacg ccaagcgtgt
      841 cgcagaattc ctgcgtgatg atcggcgcgt ggcctcggtc aactatgcgg gcttccccga
      901 aagtccctat tacccgctgg tgcagaagta tctagccggg cgggcctgct ccctcatgac
      961 tttcgcgatc cgcggcggct tcgatgccgg gacgcgattc tatgatgcac tgcgactgtt
     1021 caagcgactc gttaaccttg gcgatgcaaa gtcactggct tgccatccgg cctcgaccac
     1081 ccaccggcag atgtctcccc aggaacagag gctcgccggg atcggtccgg aaaccatccg
     1141 cttgagcatc ggcatcgagc acgtggacga tatcgtcgcc gacctggatc aggcactcgc
     1201 cgcggctgtg gcagactgaa caccacgatg agcctcttgc ccgagccggt cccggcgctg
     1261 tttccttcca gcgaggtacg gcagcgggtc gaggatgagc tcacacgagc tctgctgagc
     1321 gcccaggcgc gcctgctcgc gggcccggtg atgccggacc tggatatggc gcggttccgg
     1381 gacgagctgc gccgcttcga cttcgaggcc ccgcagccct tggacacgct gctcggctgg
     1441 gcgatcacac agctcgagca cggtgccgtg cacatggggc atccgcgcta ttttggcctt
     1501 ttcaatccgg tcgccaattt tccctcgcaa tgtgccgacc ggatctcggg cgcgttcaat
     1561 ccgcagctcg caagctcggg ttcctccccc gccccggtag agatcgaagc gcatgtgatc
     1621 cgctcgatcg cgcttcgcgc gggtctgccc gcccaggcgg gagggcattt cacgaccagc
     1681 ggctccgagg cgaattacac ggcactcgtg tgcgccctga cgcgcgctgc gccgcactac
     1741 ggaatgcagg gcgttcgggc tttcacaggt cccgtcgcga tgtatacctc gagagagtgc
     1801 cagcctgcgt ggttcaagat cgcccaccac tcaggcattg gtcgcgcctc actgaagttg
     1861 atcgctacag acggtcaggg ccgcatggac tgcaaggccc tggccgcggc aatccgcgcc
     1921 gaccgcgagc agggagtcgt gcccgtcatg atctctgcca ccgcaggcac gacgggtggc
     1981 ggcatggtgg atccgctcaa ggactgcgcc gagatcgcgc gcgagcacgg cctttggtat
     2041 cacatcgatg cggcctgggg tggggctgcg ctgtgctcgc agcggctgcg gcgcctcctt
     2101 gcaggtgtgg agctcgccga ttcgctcacg atcgatgcgc acaagtggtt cgccacgacc
     2161 atgggttgcg ggatgttcat cacgcgtgcg ccggcagtgt tgagcgagtc cttccgggtc
     2221 gcaacggact tcatgccctc cagcgtcccg agcgtggatc cctatctcaa tactctgcag
     2281 tggtcgcgcc gattcatggg gctgcgcctc tttctgtcgc tcgctgcagc gggttggagt
     2341 ggctacgcgc agcacatcga gcgggcggtc gaagtcatcg ctcaggctcg aaaccggctc
     2401 cgctccctgg ggtggtcggt cctcaatgac tctgagcttg cggtgctgtg cgtgctgcct
     2461 cccgcgggat ttcctcccgt gcgcgagctc gtccgtaagg tgctcggctc gggccgagca
     2521 tgggtggcgg catcgacctt tgagggacgc gaggtcgtcc gaatctgcaa cacccacggc
     2581 gagacttcgc tcgcggatgt cgaggagctg gtcgctgcgt tgcaggcaac gcgctagcca
     2641 atcagcctcg tgaccagcgc ctccaccgag gcgatggaat ggaagttctc cggggtgatg
     2701 tccgactgcg ggatggcgat ggagaactcg ccctccaccg ccagcatcaa gttgaccatt
     2761 ttgatcgagc tcatgcccac atcactgagt cgggcatcga tgggaagggg acgattggcc
     2821 gcgggcgacc cgaggatctg ccggactaga ccgatcaagc gctcgcgcgt cgtgcgacct
     2881 tcgtctgtct ccattgccga gctcatcatg aggcctcggc tacggcaggt gagcggaccg
     2941 gctcgcgagc agccgcgagc acggcaagcc agttgcggta gatctgagcg gctgagtcgc
     3001 gccagctcgc attcagacac tgagccacag cggcttccgg gaaggaagcc atcggatcct
     3061 gtcctgcatc ggccagtgcg cgactgcgga attcctccaa cagggcgaca ccctgatcat
     3121 tgaagtagcc gcgcgggagg gacgggtagc tcgcctgctg gccgcgcaga aagcggccca
     3181 catcacggcg gtactctttc agcagcgaag tctcgtcata ctccgggtgt ccctggaaga
     3241 acactaaaag gctgcgtccg tggcgtacaa acaggtccgc gccggactcc ggcgaggagc
     3301 tcaggatcgt gtagcctgcc gtgcgcagag cagcgaccgg gagctcgttc cagcgcgagt
     3361 gtggaataca cagtggggca ttgatctcgg ccacgagagg atgccccggc tgaagggcgt
     3421 gctcgaagac gccaaagcgt ttgtgctcga gacgctgccg cgcgatccca tccaacttga
     3481 gtaccgcggc gtgagcggcc agacacgacc atacgctcga gaccgtatgc gcctgggccc
     3541 actcgatgag ttgtccgaat cgcttccagt aaggctcctc gttcagggcg cccgggccag
     3601 gttccatgcc cgtgacgatc aggccatcca gaggctcctt gagcagcgca tcgatcggcc
     3661 agtagttgcg aagcaggtgg tccagggtct gcggagagcg cgccacctcc ggcaggtaac
     3721 acagtcgcaa ctgtacgggg agaggaccgg ccgccgactg gagcaaccgc gagaactgtg
     3781 cctcggtcgc ctcgagggcc gagtccggca tgttgttcac gagcccgaca gtgagcgcgg
     3841 ccgaggtgcg caagcgcttc gccttgcggc ggtcagccgc cccaaggagg gcgggggcct
     3901 gaatcgtgag tggcatcagc ctttcacacc aatgatcgtg atctgggtca tatagcggga
     3961 cgacgcgtgt gcgccggggc tgttcttagc gtatcgtccc gccagaaata gaacgtggac
     4021 gaacatcacc ctaatggagt cttctgtgac catcaaggcg gtgccccggc ccatggagcc
     4081 tggcccggcg gacatcccca cccgcgccca gacggtcgcc tcgatcgcgg ccgcgaacgc
     4141 cggctcggtg gacagcgcag cgcggtttcc ggccgaagca ttccgggcgg tcaaggagca
     4201 gcgcctcctc ggaattttcg tgccgaaggc actgggcggc gagggggcga gcatcgccga
     4261 ggtggccgat gtctgctacc ggctggggca gtcctgctcc tcgaccgcaa tgatctatgc
     4321 gatgcaccag gtgaaagtcg gatgtcttgt ccgtcacctg gacggtagcg cggccctcga
     4381 tcgcctggtg cgtcaactgt gctcggagca attgctcctc gcctcctcga ccaccgaagg
     4441 ccagggaggc gggaacgttc gctccagcga ggcgcccatc gagcaccaag acggccggat
     4501 cactctggag cgcaaggcga gcgtgatctc ttatggagcc tacgccgatg gggtcgtcac
     4561 gacggctcgc cgggccgccg atgccgagtc ttccgatcag gtcctcgtcg cattcttgaa
     4621 aagcgattac acgctgaccc gcctgcaggg atggaacaca ctgggcatgc gcggcacctg
     4681 cagcgaaggc tacacgctca aggcctcggg aacgcctgac caaattctcc ccgtgcccta
     4741 cgcgacgatt cacccgcaaa ccatggtgcc cttcgcccat cttctgtggg ggtccgtgtg
     4801 ggcaggaatc gccgctggcg caactgcgcg ggcacaggcc ttcgtgcgcc aggcggtccg
     4861 cggaagcggc gggcagatgc cgcccggagc gccgcagttc acgaaggctc tcacgacgct
     4921 gcgcacgctt cgcgggatgt tggccagttc gctggcggca tatcagcgcg tcatgcatga
     4981 ccccgtcgcc ctaggctcgc tcgagttcca gtccatgatc accattacta aagtcgaggc
     5041 ctccgagctc gcttgttcca cggtgatgag cgcattgcgc gcctgcggct tgtccggcta
     5101 tcgctgcgac agcgagttca gcatcgagcg gcacttgcgg gatgtcctct cctctcccat
     5161 catgatcaac aacgaccgga tcctgtcgaa tctggcgagc acagcgctga tgacgccgat
     5221 cgccgcatcc gtgcgggatt gatgacgcga ctgccctgat tgaccgaaga tgaatgacca
     5281 ttctcgagtg cccgatccga tcgagcacat cggcacggcg ttgttcagtc ccttgggtgt
     5341 ggagggggtt tacgcgcgaa ccgccctgta cgaagacatc gtggaacgtc tcgccggatt
     5401 gatctcgcga gagagggatc cgagcgccga ggtgctgcgc tttcccccgg tcatgagccg
     5461 cgagcagctc gagaagtcgg gctacctcaa gagtttcccc aatctgctcg ggtgcgtgtg
     5521 tgcgctccat ggcagtgagg cgcagatttt cgctgcggcg caatcccacg aatccggtgg
     5581 cgattggacg aaatccacgc aggtgatcga tctggttctg tcacccgccg cctgttaccc
     5641 gctctatccc atcgtcgcca agcgtggggc ggtgccacgt ggcgggctgc tgttcgatgt
     5701 cgccgccgac tgcttccggc acgaaccctc gcgcgcgctg agccgtctac agaccttccg
     5761 gatgcgggag tttgtccgca tcggatcacc ggaggaaatc acggttttcc gggaccaatg
     5821 gatgaagcgt gccgaggctc tcgccggtga gctgggtctg ccctacacga tggacgtcgc
     5881 cagtgacccg ttcttcggtc gggtggggca ggtcatggcg ctcagccaac gccagcagtc
     5941 gctgaagttc gagctgctca ttccctacta tcccggtgcg acgcgaacgg cctgcatgag
     6001 cttcaactac caccgcgaac acttcgggac cgtgtggggc cttgccgatg agcagggcga
     6061 actcgcgcac accggctgcg tggccttcgg tatggacagg ctcgcggtcg cgctgttctg
     6121 cattcacgga ttggatgttg cccggtggcc ggccgccgtg aggcgggagc tcgcattctg
     6181 acgagcacga cggcgagccc atgggcctcg acccccaaat caagcgattt ctggatcgcc
     6241 tggcggcgat gaatccgccc agcgcactcg cattgagcgt gagccagcga cgcagcgcga
     6301 tcgaaagcct cctgagcctc ggcggggaaa aggggccggt cgcccatgtg gaggagcgcc
     6361 ttcttccgat tccgggaggc agagtgggga tccggatcta cacgccgatg gatcctgagc
     6421 cgttgcccgc ggccttcgtg ttcttccatg gtggaggact ggttgcaggc agtctcgata
     6481 cccacgatgg catctgccgc tctctggctc gcgccggtcg ttgtcgcgtg atctcggtcg
     6541 attacccgct cgcgcccgag catccctttc cggcggcact ttcggcgggc tatgcggcca
     6601 cccagtggat tgcagagcac ccgcagctgc tgcaaatcga tccgcaccga ctcatcatcg
     6661 gcggtgactc agcgggggcc acgcttgcag cggtcatctg ccaaatggcg acgacgcgta
     6721 aggaggttcg gtttgcgctc caagtgctgc tgtgccccat tgtggacttc tcggcctcca
     6781 ccccctcgag atgcgaattc gcccaggggt atctggtgga cgaggcgaca ctcgctcacg
     6841 atgtgaagca ctacctgggt ccggatgcgc ttgccaccga cccgcgggtc tccccgggtc
     6901 gggctgccga tgtcgctgat ctgccgccga cgtgcatcca cacggccgaa ttcgatccgc
     6961 tgcgcgatga ggggaaggct tatgccgagc ggcttcagca ggccggcgtg aaaacgacct
     7021 accggtgcca ctccggtatg attcatctgt tctacgggat gggagcgctg attccgtccg
     7081 tcgcccgtgc ctaagagctg atcggggccg atatgcgctc gctgctcagg ccgcctactt
     7141 tgcaggggag tgttcgatga cgtcccgttc cgaagtcgag caacttctgc gggcgctgta
     7201 tgcgacgaga atcgcgggac ggcgcgatga gctctgcgcg ctcttcgccg agcagagccg
     7261 cttccggatc ggaggcacca gcgatggcaa acccatttcg gtttccgcca acggtgctgc
     7321 cgagatacgg acttggctca caatgttcgt aaagaccttc aagatcagtg agcacgagat
     7381 cctctcgatg gtcatcgagg gcgagcgggc cgcagtgcac tggcgcgcca acatccactc
     7441 caggatcacg gggacgctcg ttcccacgga gctcgtggac ctcatcgaag tgcgcagtgc
     7501 acacatcaca tcctatgtgg agttttttct gcccgtctga agttgcccgc ggcatccagc
     7561 ccttcctcgg ccgccgggtc attatgcccg cgggaaaggg tgcgatttct cacataagaa
     7621 tgggccgcat gtcggataaa atcgccctct acgctcaggg gggcgtcgat gcaatacaca
     7681 tcaggtcggg gtgtcctgag cacatggccg tgagtttccc actcagcccg gatagagcct
     7741 cctcgctagg cgaggcgctc tatttcgact ccggcgagca ccggctgttc ggctggctgc
     7801 atcgtgcggc agcggagcag ggcgcgaagc tgggcctggt gatctgcaag cctttcggtt
     7861 acgaggcgat ctgttcacac cgcggcgtcc gcttgttcgc cgagtcggca gcggcgctgg
     7921 gtgtgccgac actgcggttt gattacgccg gggcggggga ttcctgcgat attcagccga
     7981 cggacgatca gatcgagatc tggacccagg acgtgctcgc cgcggtgcag gagctgcagc
     8041 gccagaccgg tgtgcagcaa gttggtttgc tcggatttcg actgggcgcg ttgctggcga
     8101 tcctcgccgc gcgcaagagc acggcgattc gcgcactggc tctcgtcgcg cctgtcatca
     8161 gcggacgccg ctacattcgc gagctgcgaa cgacgcgact tgccgccgca ctcgccttgg
     8221 accccggcaa tgccgccaag cctcccgaga gtgaggccgg ctccatggag gtcagcggat
     8281 tctcactctc ggctgcctca ctcgcctcac tgggcgcgat ggatctggca acaatcgatg
     8341 cgcccgcgat ctccgccgtc cttgtgattg atcgcagcga ccttccgcag tgcgctcagt
     8401 tcgccgagaa actgtcagga tccggcatcc ggactgaata tcacagcatg gcgggactgg
     8461 tcgggatgct gatgaccgac cctgagttcg cagcgacgcc ggagctcacc gtgagcgtga
     8521 tggggcgctg gctggaacgg caaagggatg gggctggagc actgagcggg gcgaccgccc
     8581 aatcccccgt cagctcgagc ccgcagatgc cctcattagg attgccgggc gagggcgagg
     8641 ccgcactcag cgagcacccg gtcttctttg gctctccggc gacgatgttc ggaatcatta
     8701 cggagcccgc gccaggggag atgcggcgcc gggcagtcat tctgctcaat gtcggggcgg
     8761 aacatcacat tggctccagt cgcatccacg tctcgctcgc tcggcgctgg gctcgtcatg
     8821 gctacacggt cctcaggctc gacttgacgg gcctgggcga cagcgagacc cgtcctgaca
     8881 ggcccaccaa cgaggtgttt cccgcgacgg ctttcgagga catccggatc gcgatggagg
     8941 ttctacgcag ccgctatggc atctcggaca tcaccctggg cggcttgtgc tccggggcat
     9001 atcactcgct gcgcgccgcc atcatggggc tacccgtgaa ccgggtactg gttgtgaatc
     9061 cgatgaattt cttctggaaa gaagggatga acgccgagac cctgcagcag gatgtagacg
     9121 ttgctcgcaa tctcggcttc taccgcgacc ggctgctgtc gagcgtcatc tggaagcgga
     9181 tcttcagtgg agagcttggg atcggccgcc tcgtgcgcat ctgcctcaag cgccccttgc
     9241 tcgcgctgga atcatttttc cgcggcgctg cacgaacttt tcgcatccga ctgcccaacg
     9301 atctgggctg ggagctcgaa gagattgccg ctcgaggggt ccgaaccgta ttcgtgttct
     9361 ctcgcgggga gccagggatc gaccggctga agatgcaggc gggctcgacg gtcaagcggc
     9421 tgggcgatct ttgccgcgtc cacatcatca acagcgccga tcacattttc tcgcaggccg
     9481 cgcagcgcac gaccctggag agcatcctca cggatgagct gttcgcccgc aaccaacggg
     9541 ccaaccccac gctggcccgg ggcctggaga tccagcccgc cggcaactcc ttgcccggca
     9601 agcactagaa ttcgtccggg aggcagcatg caggccgcta gagaactggt acgagagagt
     9661 ttcctgccac tgggtcgcat gagcgagctg gcaaagtccg ctcacgagtc gtacctccgg
     9721 gcccagccct atccacacat cgtgttcgac aacttcttca atccggagct gttggatatc
     9781 gtgctggagg agtttcccaa gccgggtgcg attcgctggc agaagttcga caacgcgcag
     9841 gagatcaagc tcgcctccgc ggcggaggcc tcattcgggg cggtcacccg gctgctgatg
     9901 tatcacctga attccattac ctttctcgaa ttcctgagcg cggtgacggg tatcgagaac
     9961 ctcatacccg atccgcaatt cgaaggcggc ggccttcacc agattgtgcc gggcgggaag
    10021 ctcggggtgc acgcagactt caacaagcat cgcgtgttcg gcctcgaccg gcgcctgaac
    10081 gtactcatct acctgaacaa gaactggcgc gaggactatg gcggatacct ggagctgtgg
    10141 ggtcgagaca tgacacgctg cgaggctagg gttctgccgc tattcaatcg catgatgatc
    10201 tttggcacga ccgatttcac ttaccacggc catcccgatc cattgaagtg cccggagggc
    10261 atgacgcgta aatcgctcgc tctttattat ttcacgaatg gccgccccgc cgaggaaatc
    10321 agcggcgagc attccactga gtttcgtgcc cgcaaggcgg atgattttgt tccgaccttc
    10381 cgccagcgcc tcagaggtgt ggcgcgagag ctgctcccgc ccatcatcag ccggcgtctg
    10441 cgcggagtgc tctgaagcgc tggacctctc atggagctcg ggctggtagt catcggacgg
    10501 aacgaaggcg agcggctgat gcgttgcctg gcgtccgtgc gtgagattcc caggaaggtt
    10561 tacgtcgatt ccggctcgac tgatggaagt gttgagagag caggtgcgca aggcgtgacc
    10621 gtcgtcgagt tgccggtccc accgaacttt accgctgcgc gcgccagaaa ttcaggtctt
    10681 gcgatgctcc ttacgcaaaa tccagagctc gagttcgtgc agatggtgga cggagattgt
    10741 gagatccgag ccggttgggt cgaggagggt ctggcagtgc tgcgtgcgga cccgaaggtt
    10801 gcaacggttt tcggccgact gcgcgagcga tttcccgatc tgtcggtcta taacgctctg
    10861 tgtgatgatg aatggaacat tccgatcggc gacgccaccg gcagcggggg agtggccttg
    10921 ttcagagtat cggcattgcg gcaggtggac ttctacaatg aagccatgat cgctggggag
    10981 gactcggaac tgtcgatgcg tttgcgcaag cgcggctggc gtttgcgaag gatcgacgtg
    11041 gaaatggctg tgcacgatgc tgcactgact cgattcagcc aatggtggac gcggacccgc
    11101 cgctcgggac acggctttgg cgaaatggcg tttctgcacc ctgatgcgcg aagcccgaac
    11161 tggccccgct cggtgcgcag catcgtcttc tggggcggcg cgatgcccat cgttcttgcg
    11221 atcgcatgcg tcctgggcgt gcttttcgga ggctcctggt gggctctcgc agcactggtg
    11281 ctgctcgtgt ggcccgcgcg aatgacacag cttgcgatcc gacagtggcg ccgcggcctc
    11341 agcatcaagg tggcctgcgc gtccggtgtt ctgctcatgt tgggtaagat cccgcaactc
    11401 atcggtctgg cgggctttca tcgcgatcgg ctgactgggc gtgcctcgag tctcatcgag
    11461 cacaagcgtc cccaacgcgc ctgatcggaa tttagaggca gatgcaggta ggctcaggct
    11521 gaagcggcca taatcgttcc cgagccgctg gaaagcgacg gcccttccac acaggaatac
    11581 tggtttgaca gcccccaaga aggtcggact gctcggcgcc ggctacatac tcgattcgca
    11641 tgccaaatcg cttgcggccc tgccaggtgt ggcgctgcat gccgtctgcg atgtctcgcg
    11701 agggcgggcg gcccaggcgg cagctcggtt ctcgataccg cacgtgttca gctccattac
    11761 ggagctggcc ggttccgact gcgacgtcgt tcacatcttg ttgcctcccg gcctgcatat
    11821 cgaggcagcc tcaaccctgg tggaagcagg caaatcggtg ttcctcgaga agccgatggg
    11881 cctggacagc gatgcctgcg aggccctcag ccggcgagcc catgagaggg ggttggcgct
    11941 tggtgtgaac cataacttcc tgttcacccc gggctacgag gcactcagga gcagtctcaa
    12001 ggcgggcgag ctgggtcgcc tcgatcatat cgccttgaac tggcacttcg cgcttccgat
    12061 cctgcagttc ggcccgttcg atagctggat gttggctgct cctgccaacg ttcttttcga
    12121 gatcggtgct cacttgtgcg ccttcgtcgt cgacctcgtc ggcgtgccgc agatcgattc
    12181 cgcggttgcc ggcaatccca tttcgctccc ggccaatcag acggtgtacc gccaatggac
    12241 tgcgcgggcc catgctggct cagcgacggt ccttctgtcg atctcgctga cggcgggtca
    12301 ggcggatcga attcttcggg tgcggggccg cggcggcagc gcacaactcg acttcggccg
    12361 cgacttcggc tggtgcgatc tcacggtgac ggacaatccc atct
//
LOCUS       DASRHN010000166         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1375349, whole genome shotgun sequence.
ACCESSION   DASRHN010000166 DASRHN010000000
VERSION     DASRHN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375349"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFB37_06540"
     CDS             complement(<1..523)
                     /locus_tag="VFB37_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005169709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO22148.1"
                     /translation="MTETLELTRDLMARRSVTPADEGCQQLLAARLQRAGFQIEHLPF
                     GEVENLWARRGTEAPLFCFAGHTDVVPAGPLEEWSSDPFVPVIRNGALYGRGAADMKS
                     GLAAMVTAAEEFVAAHPRHKGSLAFLITSDEEGPSVDGTQRVIEKLTGRGEKIDWCLL
                     GEPSSEVSVGDTIK"
     gene            799..1842
                     /gene="pstS"
                     /locus_tag="VFB37_06545"
     CDS             799..1842
                     /gene="pstS"
                     /locus_tag="VFB37_06545"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZO22149.1"
                     /translation="MSLVNPKRLLALLATVSVAASAQAATDISGAGATFPYPVYSKWA
                     DAYKKQTGVGLNYQSIGSGGGIKQIKAKTVTFGASDKPLEPAELKAAGLVQFPMIIGG
                     VVPVTNVKGIKAGQLTLDGATVASIYLGEITEWNDARIKKLNPSAALPATAIAPVYRS
                     DGSGTNFLFTDYLSKSSPKFKQSIGANASVQWPVGIGAKGNEGVANMTTQTDGAIGYV
                     EYAYAKQNNMAFAKLINRGGKAVAPSAETFQAAAANADWAHSPGYYLILTDQPGAASW
                     PITGASFILLYGTPPNPGDTAAALKFFDWAFRNGGQMASELDYVPLPESLIKQIRATW
                     KSDIKGIPAMAAN"
     gene            1853..2824
                     /gene="pstC"
                     /locus_tag="VFB37_06550"
     CDS             1853..2824
                     /gene="pstC"
                     /locus_tag="VFB37_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZO22150.1"
                     /translation="MNSAVIEADSRHAAGQRLRESIFRGATLVAALLVLTLLGGVALS
                     LLKGAWPAFSHFKLQFLTREIWNPVTDQYGALAAVYGTLVTSLIAMLIAIPISFGIAI
                     FLTELAPPWLKRPVGVAIELLAAVPSIIFGIWGLFVLAPMLQRHVQPWLIDVLGPLPL
                     IGKLFQGPPFGIGVLTASVVLAIMVLPFISAVMRDVFETVPDVLKESGYGLGATTWEV
                     IWKVVLPYTRSGVVGGIMLGLGRALGETMAVTFVIGNAHRISPSLLGPGTTISASIAN
                     EFTEAVGDLYTSSLIGLGALLFLITFTVIAAARLLLLRLDRRALSSV"
     gene            2821..3675
                     /gene="pstA"
                     /locus_tag="VFB37_06555"
     CDS             2821..3675
                     /gene="pstA"
                     /locus_tag="VFB37_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZO22151.1"
                     /translation="MTRRGIRKITNVVALGLSGLATAVGLFFLGAILWTLIGHGLAGM
                     SLSLFTQNTPPPGSSGGILNAIYGSVVMTLIGILIGAPVGMLAGTYLAEYGRGSRLST
                     VIRFVNDVLLSAPSIIVGLFIYEILVVRMGHFSALAGAVALAVIAIPVMVRTTEDMLN
                     LVPQGMKDASTAMGAYPWRTITNIIYPAARSGIVTGLLLAIARISGETAPLLFTALNN
                     QFWSTNLRAPIANLPVIIFQFALSPYKNWQQLAWSGALLITFAILVLSITARIVVSAL
                     HPAGSRQR"
BASE COUNT          638 a         1269 c         1201 g          714 t
ORIGIN      
        1 ccttgatcgt atccccgacc gagacttcgc tggagggctc cccgagcagg caccaatcga
       61 ttttctcgcc gcgtccggtg agcttctcga tgacgcgctg agtgccatcg accgagggcc
      121 cttcttcgtc actggtgatg aggaacgcga gggagccttt atgacgagga tgggcggcta
      181 cgaactcttc ggccgcggtc accatggcgg ccaggccgct tttcatgtcg gccgcaccgc
      241 gcccgtagag cgcaccgttg cggatcaccg ggacgaaggg atcactgctc cactcctcga
      301 gcggaccggc aggcacgacg tcggtatggc cggcaaagca gaagaggggg gcttcggttc
      361 cgcgccgtgc ccacaggttc tcgacctcac cgaagggcag atgctcgatc tgaaatccag
      421 cccgctgcaa ccttgccgcg agcagctgct ggcagccctc atccgctggg gtgaccgagc
      481 gtcgtgccat caagtcgcgg gtcaattcga gggtttcggt catgctcagc ttcccgggcg
      541 caaaacggtg gcgcctgcgc tccagcagag gcctcccggg tcgcggcggc tcgcgttata
      601 aggcccggcg cgctcgccgg caagccgcac gcacccgggc aggctccggg ggacaccccc
      661 gcaccccgct gtcatcaaaa cgtcatattc cggtcatgta atggctaatc agcgtgcgcg
      721 atgcttgccc gggccagtcc gcgggggcgc tcagaaaacc accacaggcc gcgggaacac
      781 catgcggagg atccactgat gagtcttgta aatccgaagc gactgctggc gctcctcgcc
      841 accgtgtccg tggcagcctc agcgcaggcg gccaccgata tatcgggcgc cggcgctacg
      901 tttccgtatc ccgtctactc gaagtgggcc gatgcgtaca agaagcagac cggcgtcggc
      961 ctgaactatc agtcgatcgg ctccggcggt gggatcaagc agatcaaggc gaaaacggtc
     1021 accttcggcg cctccgataa gccgctggag ccagcggagc tgaaggcagc cggtctggtg
     1081 cagtttccga tgatcatcgg cggcgtcgtg ccggtcacga acgtcaaggg catcaaggcg
     1141 gggcagctca cgctcgatgg tgcgaccgtc gcgagcatct acctgggcga gatcaccgag
     1201 tggaacgatg cgcggatcaa aaagctcaat ccgtccgcag cgctgcctgc aaccgccatt
     1261 gcgcccgtgt accgctccga tggctccggc accaacttcc tgttcacgga ctacctttcg
     1321 aagtcgagcc cgaagttcaa gcagtcgatc ggcgccaacg cctcggtgca gtggcctgtt
     1381 ggaatcggcg caaaaggtaa cgagggcgtg gccaacatga ccacgcagac cgacggggcg
     1441 atcggctacg tcgaatatgc ctatgcgaaa cagaacaaca tggcgttcgc gaagctcatc
     1501 aaccgcggcg gcaaagccgt ggctccgagc gcagagacct tccaggcggc tgccgcaaac
     1561 gcggactggg cccattcccc cggctactat ctgatcctga ccgatcagcc gggtgctgcg
     1621 agctggccga tcaccggtgc cagcttcatt ctgctctacg ggacgccgcc gaatccgggc
     1681 gacactgccg ccgcgctcaa attcttcgac tgggccttcc gtaacggcgg ccagatggcc
     1741 tcggagctgg actacgtacc gcttcccgag agcctgatca agcagatccg cgccacctgg
     1801 aagtcggaca tcaaggggat cccggccatg gcggccaact gacggcacgg cagtgaacag
     1861 cgctgtcatc gaagcggatt cgcggcacgc tgcgggccag cggctcaggg agagcatctt
     1921 tcgcggcgcc actctggtcg ccgcgctgct ggtgctgact ctgctggggg gagtcgctct
     1981 gtctttgctc aagggggcgt ggcccgcgtt ctcgcatttc aagctgcagt tcctgacgcg
     2041 tgagatctgg aatccggtca ccgaccagta cggcgccctc gcggccgtgt acggcacgct
     2101 ggtcacttcg ctgatcgcaa tgctcatcgc gatcccgatc agcttcggga tcgcgatctt
     2161 tttgaccgag cttgcgccgc cgtggctcaa gcggccggtg ggcgtcgcca tcgagctgct
     2221 cgccgcggtg cccagcatca tcttcggaat ctggggcctg ttcgtgcttg ctccgatgct
     2281 gcagcgtcat gtgcagccct ggctcatcga tgtgctgggt cctctgcccc tgatcggcaa
     2341 gctcttccag ggacctccgt tcggaatcgg tgtgctcacc gcttcggtgg tcctggcgat
     2401 catggtcctg ccgttcatct cggcggtcat gcgggatgtg ttcgagacgg tccccgatgt
     2461 gctcaaggaa tccggttacg ggctgggtgc caccacctgg gaagtcatct ggaaagtcgt
     2521 gctcccctac acacgttccg gcgtcgtggg cggcatcatg ctggggctgg ggcgcgcgct
     2581 cggcgagacc atggcggtca cgttcgtgat cggcaacgct catcgcatca gcccgtcgct
     2641 gctcgggccc ggaaccacga tctccgcttc catcgccaat gagttcaccg aggccgtcgg
     2701 ggatttgtat acgtcctcgc tgatcgggct cggcgcgctg ttgtttctca tcaccttcac
     2761 ggtgatcgcc gcggcgcggc tgctgctgct gcgcctcgat cggcgcgccc tgtcatccgt
     2821 atgacccggc gcggcatccg caagatcacc aatgtcgtgg cgctcggctt gtcggggctg
     2881 gcgacagcgg tcgggctgtt cttcctcggc gcgatcctct ggactctgat cggccatgga
     2941 ttggctggaa tgtcgctcag cctgttcacg cagaacactc cgccgcccgg cagcagcggc
     3001 ggcatcctca atgcaatcta cggcagcgtg gtcatgacgc tgatcgggat tctgatcggc
     3061 gcgcccgtcg gcatgctggc ggggacctat ctcgcggagt acggccgcgg ctcgcgtctc
     3121 agcaccgtga tccgcttcgt caacgatgtg ctgctgagcg cgccctcgat catcgtcgga
     3181 ctgttcatct acgagatcct ggtcgtgcgg atgggtcact tctcggcgct cgccggcgcg
     3241 gtcgcgctcg cggtgatcgc cattcccgtg atggtgcgca cgacggagga catgttgaat
     3301 ctggtcccgc agggcatgaa ggatgcctcc acggccatgg gggcgtatcc gtggcggacc
     3361 atcaccaaca tcatctaccc cgccgcgcgc agtgggatcg tgaccggcct gctgctcgcc
     3421 atcgcccgca tcagcggcga gaccgcgccg ctgctgttca ccgcgctcaa caatcagttc
     3481 tggagcacca atctgcgcgc gccgatcgcc aatctgccgg tgatcatctt tcagttcgcg
     3541 ctcagcccct acaagaactg gcaacagctc gcctggtccg gtgcgctgct catcaccttc
     3601 gccattctgg tactgagtat caccgcgcgc atcgtcgtct cggcgctcca tccggccggc
     3661 tcgaggcagc gatgagcgcc ggttcgaggc agccatgaac gcaagcgtcg cgagcgtgcg
     3721 cgcgaacggc cattccatcg aggcgcgcgc cccgaaattc gaggtccgtc acctcgactt
     3781 ctactacggc cgcaaccgcg cactgacaga catcaatctg ag
//
LOCUS       DASRHN010000167         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1210815, whole genome shotgun sequence.
ACCESSION   DASRHN010000167 DASRHN010000000
VERSION     DASRHN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210815"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..2119)
                     /locus_tag="VFB37_06560"
     CDS             complement(113..2119)
                     /locus_tag="VFB37_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZO22152.1"
                     /translation="MIDSLLVANRGEIACRIFRTARRLGIRTIAVYSDADARARHVRE
                     ADEAVRIGPPAPRSSYLDITQILAAARTSGAAAVHPGYGFLSENARFAQACIEDGRIF
                     VGPPASAIAAMGSKIVAKARMRTAGVPVLPGYEGCEQDLEHLVIEARRLGMPLIIKPA
                     AGGGGKGMRIVREEGQLAEALAAARRLAESGFGDGALLLERFLPAPRHVEVQIFADTH
                     GNFIHLGDRDCSTQRRHQKLIEEAPAPALPEELRTRLRAAALVVAREIGYVSAGTVEF
                     LFDGREFYFMEMNTRLQVEHTVTEAISGIDLVEWQLRIAAGEPLPLRQEQIQLNGHAI
                     EARVCAEDPERDFLPSAGLLRLMEWPSAEPVRVDAGFCTSDTVPETYDSLLGKIIAWS
                     PSREQAAALLARALRKTYSAGVRTNERWLARVLVSPDFLAVRHSIAFLQEHAAEFADR
                     AEPGPTALVFAALIAHQGAVPVSTGAGAASPWSERDGYIPNLEAGVSLLLSWRTREIP
                     VEVHFARGTATEVVMETGGASGVPIRIEQALVSGDTISAVLDDERLHARYFVSGSHIH
                     LWFGGEHHEFVLQDPRTLEFTATAARGGLTTPLPGIVVAVAVQPGQAVKAGEVLMVIE
                     AMKMEHTITAPHDGTVQAIHFARGERVAEGSELLEIAPADIKKS"
     gene            complement(2144..>3268)
                     /locus_tag="VFB37_06565"
     CDS             complement(2144..>3268)
                     /locus_tag="VFB37_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZO22153.1"
                     /translation="HFGRIFYNQATLSGSGIAQIAVVHGSCTAGGAYVPAMSDENVIV
                     RNQGRVFLGGPPLVKAATGEDIDAESLGGAEVHCRESGVCDHYAENDTHALSIARRIV
                     ARLAPPSNSLPPHPSREPRYAAEELYGVVAASLRKPYDVREVIARLVDASELDEFKQL
                     YGTTLVCGFAHIGGYPVGILANNGILFSESSLKGTHFIELCARAGMPLLFLQNITGFM
                     VGRRAEAGGIAKDGAKLVTAVACADVPKLTVIIGGSYGAGNYAMCGRAYSPRFLFQWP
                     NSRISVMGGEQAASVLATVKGDQMKAAGREWASSEEEAFKQPIRDQYERQGHPYYASA
                     RLWDDGVIDPAETRRILTLCLAAVRHAPGTTETRFGVFRM"
BASE COUNT          590 a         1104 c         1031 g          543 t
ORIGIN      
        1 tagcggtctg cacaatctcc atcgactcca gccgatcgac aacgctcacg tggaggcagt
       61 aactgcctca gagactctga cgcgagcgcg gcaaatatct caccggggcg gattaacttt
      121 tcttaatgtc cgcgggagcg atctcgagga gctcactgcc ctcggcgacg cgctcaccgc
      181 gcgcgaagtg gatcgcctgc accgtgccgt catgcggggc tgtgatggtg tgctccattt
      241 tcatggcttc gatcaccatc aagacctcgc cggccttgac cgcctgcccg ggctgtaccg
      301 cgaccgccac gacgatgccg ggtagcgggg tcgtcaggcc gccgcgcgca gccgttgcag
      361 tgaactcgag ggtgcgcgga tcctgaagca cgaattcgtg gtgctcaccg ccaaaccaga
      421 ggtggatgtg gctgccgctc acgaagtagc gggcgtgcaa ccgctcatcg tccagtaccg
      481 cgctaatcgt gtcgccggaa acgagtgcct gctcgatccg gatcgggaca ccggacgccc
      541 cgcccgtctc cattaccact tcggtggccg tgccccgggc aaagtgaacc tcaaccggga
      601 tctcgcgagt gcgccagctc agcaggagcg aaacaccggc ctcgaggttt ggaatgtacc
      661 catcccgctc gctccaagga ctggccgcac cggctccggt gctgaccggc acagcgccct
      721 ggtgtgcgat cagcgctgcg aagacgagcg cagtgggacc tggctccgct cgatcggcga
      781 actccgcggc atgctcctgc aggaatgcaa tgctgtgacg aaccgcgagg aaatcgggag
      841 agaccagaac gcgtgcgagc catcgctcgt tggtgcgcac gccagcgcta taggtcttgc
      901 gcagagcgcg tgcaagcagt gctgcagcct gctcgcggga gggcgaccag gcgatgatct
      961 tcccgagcag cgagtcatag gtctcgggca cggtgtccga ggtgcagaag ccggcatcca
     1021 cgcggaccgg ctcggcgctg ggccactcca tcagtcgcag gagtcccgca ctcggcagaa
     1081 aatcccgctc gggatcctcc gcgcagaccc gagcctcgat ggcatgtccg ttgagttgaa
     1141 tctgctcctg ccgcaggggg agcggctcgc cggcggctat gcggagctgc cactcgacca
     1201 ggtcgatccc gctgatggcc tcggtgaccg tatgctcgac ctgcagccgt gtattcatct
     1261 ccatgaaata gaactcgcgg ccatcgaaca ggaattccac ggttccagcg ctgacgtaac
     1321 caatctcgcg cgccacgacg agcgccgccg cgcgcagtcg cgtgcgcagc tcctccggca
     1381 gtgccggagc cggcgcctcc tcgatgagct tctgatgccg gcgctgggtg gagcaatcac
     1441 gatcgccgag atggatgaag tttccatgtg tgtcggcgaa tatctgcact tccacgtggc
     1501 gcggggccgg cagaaatcgc tcgagcagga gcgcaccatc tccgaaacca ctttccgcga
     1561 gccggcgtgc ggccgccagc gcctcggcga gctgtccttc ctcgcgcacg atcctcatgc
     1621 ccttgccgcc gccccccgcg gcgggtttga tgatgagcgg catcccgagc cgccgcgcct
     1681 cgatcaccaa atgctcgagg tcctgctcgc agccttcata gccgggcagc acgggcacac
     1741 cggcggtgcg catgcgcgcc ttggcgacga tcttcgagcc cattgccgca atcgccgagg
     1801 cgggcggtcc cacgaagatc cggccatcct cgatgcaggc ctgcgcgaac cgggcgttct
     1861 ccgacaaaaa gccgtaaccc gggtggaccg cggcggcgcc actggtgcgc gccgcagcaa
     1921 gaatctgcgt gatgtcgaga tagctcgagc gtggggcggg cgggccgatc cgcaccgctt
     1981 catccgcctc ccggacgtgt cgggctcggg catcggcgtc ggaatacacg gcgatcgtcc
     2041 ggatccccag ccgtctggcc gtgcggaaga tgcgacacgc aatctcgccc cgattcgcga
     2101 ccagcaaact gtcgatcaca tctgaccctc gatcgccgcc gaactacatt ctgaacacgc
     2161 cgaagcgcgt ttccgtcgtg cccggcgcat ggcgaaccgc agccaggcag agcgtgagaa
     2221 tacggcgcgt ctcggcgggg tcgatcaccc catcatccca aagtcgcgcg gaggcatagt
     2281 agggatgtcc ctgacgctcg tattgatcgc ggatcggctg tttgaaggcc tcctcttcgg
     2341 agctcgccca ttcgcgtcca gcggccttca tctgatcgcc cttcacggta gccagcacgc
     2401 tggcggcctg ctcaccgccc atcacggaga tgcgcgagtt gggccactgg aacaggaagc
     2461 gtggcgagta tgcacgccca cacatcgcgt agttgcctgc gccatagctg ccgccgatga
     2521 tgacagtgag cttggggaca tccgcgcacg ccacggccgt cacgagcttg gcaccgtcct
     2581 tggcaatgcc gcctgcttcg gcacgccgac ccaccatgaa tccggtgatg ttctgcagga
     2641 acagcagcgg catgccggcc cgggcacaca gctcgatgaa atgcgtcccc ttgagcgagc
     2701 tctccgagaa caggatgccg ttgttcgcaa ggatgcccac cggatagccg ccgatgtgag
     2761 cgaaaccgca caccagcgtc gtgccgtaga gctgcttgaa ttcatcgagc tccgaggcat
     2821 ccaccaggcg tgcgatgacc tcgcggacat cgtagggctt gcgcaagctg gcggcgacaa
     2881 ctccatacag ttcctcggca gcgtagcgcg gctcgcgtga gggatgcggc ggcaggctgt
     2941 tcgagggtgg cgccagcctg gccacgatcc gacgcgcaat ggacagcgca tgggtgtcgt
     3001 tctccgcgta gtgatcgcag acgccggact cacggcagtg cacctccgcc ccgcccagcg
     3061 attccgcatc gatgtcctca ccggtcgcag ccttcaccag cggcgggccg ccgaggaaca
     3121 cccgaccctg gttacgcaca atcacattct catcggacat ggcaggcaca taggctccgc
     3181 ctgccgtgca ggagccgtgc accacggcga tctgagcgat gccagagcct gaaagggtcg
     3241 cctggttgta gaaaatgcgc ccgaaatg
//
LOCUS       DASRHN010000168        10404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_547176, whole genome shotgun sequence.
ACCESSION   DASRHN010000168 DASRHN010000000
VERSION     DASRHN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547176"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFB37_06570"
     CDS             complement(<1..760)
                     /locus_tag="VFB37_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22154.1"
                     /translation="MKAPQATTTILAICAAISSALALAQQPPDVVVSDGNENTAMGTG
                     ALQSVTPNSCLYCGIENTAAGYEALYYDTAGGGNVAMGFLAMEFNETGHNNTAVGDEA
                     LQHNSGGYYNTAFGSGAVHENLSAFYNTGIGLNALFHNTSGADNTALGAYALTSSTLS
                     IENTAVGYQTLFTDTSGAYNTASGSQAMYANTTGYQNTASGYQALYGNTLGNHNTVAG
                     FQALFSNSNGNSNTAAGDQALYYNTTGYHNTVLGA"
     gene            complement(898..1278)
                     /locus_tag="VFB37_06575"
     CDS             complement(898..1278)
                     /locus_tag="VFB37_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22155.1"
                     /translation="MRTDTRLDLLAPLTLLGSLLGLVAPALVDPSTAGPDSSGTGSFA
                     ADWLATSDAAKEAQPQWMTPLVTVTPRLEQEIRYDQSWQERPKDVARDNYGNTKGLEL
                     IPSLNSELLLFPWLRAPFCVRTRR"
     gene            1529..2629
                     /locus_tag="VFB37_06580"
     CDS             1529..2629
                     /locus_tag="VFB37_06580"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZO22156.1"
                     /translation="MAEDGTNLISMPAANSPNAFDLRRVGAHPDHWYALAWSDELKSG
                     SVLGRRFADLPIALYRGKGGRVFALEDRCAHRQVPLHLGVVSGDHIKCHYHGWTYDGS
                     GKCIDIPYLGKERLPNGVRSYPAREVDGLIFVFPGDPALAEARLPRTLGSSQRKEYKT
                     RRLNREVACHYTFMHENLFDMNHQFMHRKQMGSIRARCLGRQHGENWAEVEYTFTRTE
                     GKQSVGEQVIVDLVRRRGQSKQDFSDHMRIRTDYPTQSLKVWVGRNINDAQDPVLDVW
                     LSYTPLDAQQRTNRTFGYLSVRKPPIPGLIHIVWPFVTWFTERIFAEDKDIVEHEQRA
                     YDSQGADWNNEVFPAIRDLRAVLARCGAPIHD"
     gene            complement(2653..3444)
                     /locus_tag="VFB37_06585"
     CDS             complement(2653..3444)
                     /locus_tag="VFB37_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZO22157.1"
                     /translation="MIVTSVMPLPAPEEIHLWFVFCDRIRDRTLLARYRELLSAQERE
                     QEKRLLQPRGRHRYLLTRALVRTTLSRYAAVAPEDWLFASDEYGRPRILNERPDTADL
                     SFNISHTEEVVLLGVANRYALGVDVEKVRRSTPVLQLAQRYFSAIETRGLHEQEAARQ
                     QQRFFEYWTLKESYIKARGLGLSIPLDQFSFHIADHELSLQIEPQSNDAADRWRLWLL
                     RPCPEHLAAVCAERILPAQSLVARAVVPLHSEHAMTCELLARSPA"
     gene            3653..4711
                     /locus_tag="VFB37_06590"
     CDS             3653..4711
                     /locus_tag="VFB37_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22158.1"
                     /translation="MQRPVVLLLVTLAFAGTSKECAAAGSDNSIPEQPAPADSTSSRQ
                     ARDAAWWTGPLLAASAGTLPQGHFLIEPYVFDSMPQAHYDPHGDRQPLAHANNFGSQS
                     YVLYGFTERISAGLIPRFGFNDPGTARPAAHLGVGDLGLQAQYGLTRFEEGSWIPTTS
                     LVLGETLPTGQYDRLGDRPADGVGSGVYTTTLGIYSQYVFWLPTGRILRTRLDLSYAI
                     PGSASVQDVSVYGTSQGFRGRAAPGNTFIADLAGEYSLTRSWVLALDIAYEHDASTQL
                     NGLQLAAAASGSGSPVGLPPAAIALSSGPSHSLSLAPAVEFNFNSRVGVIAGAKLTAS
                     GRNINSAVIPVAAVNIVL"
     gene            4946..5476
                     /locus_tag="VFB37_06595"
     CDS             4946..5476
                     /locus_tag="VFB37_06595"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HZO22159.1"
                     /translation="MNGEVGGDAVRATRRAVTQSPAVSQAHAADRRGSDRRQRTLHAL
                     IYGSFHPRRRQPRRAGEAILSGIDWHAPQWLAIAVLIVVLSCVDAALTLALLQLGAYE
                     VNPVMAALVGSSAAAFTAVKVGLTGGGVVLLTLLARMRAFGKIPVGLILYLVLLGYGV
                     LVMYEVHLLDQTPRPF"
     gene            5579..8365
                     /locus_tag="VFB37_06600"
                     /pseudo
     CDS             5579..8365
                     /locus_tag="VFB37_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            8482..9789
                     /gene="odhB"
                     /locus_tag="VFB37_06605"
     CDS             8482..9789
                     /gene="odhB"
                     /locus_tag="VFB37_06605"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HZO22160.1"
                     /translation="MTTEIRVPQLPESVADATLVAWHKQPGDAVRRDENLADLETDKV
                     VLEVPAPLNGVVREIRIPSGTTVTSGEVLALIDEGAQPAASAGAPAAAKAPASPSQAS
                     PAPQASAPKPASTAKETDNGSARDLGKLSPSVRRLVEENRLDPDSIPASGKDGRLTKA
                     DVVEFLEKRPATASPEPTASPATLSPPAAARADAPGAKPASGARAQQRVPMTRLRQRI
                     AQRLVEAQATQALLTSFNEVDLTAVQDLRARYKDRFEKEHGIKLGFMAFFVKASIEAL
                     KKFPIINASVDGTDILYHEYYDIGVAVSTDRGLMVPIVRDADAKSFATIEKEIAQFAR
                     KAREGSIGIEDLTGGTFTITNGGVFGSLMSTPIVNAPQSAILGMHKTQERPMVVNGQI
                     AIRPMMYLAVTYDHRIIDGREAVQFLVAVKEGLEDPGRMLLGI"
     gene            9796..>10404
                     /locus_tag="VFB37_06610"
     CDS             9796..>10404
                     /locus_tag="VFB37_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO22161.1"
                     /translation="MSDSFDVVVIGGGPAGYPAAIRAAQNKLSVACIDEWKNHDGGAA
                     FGGTCLNAGCIPSKALLESSELYHRVREEFAVHGIRTTGVSLELAQMQKRRAGIVRTM
                     TQGILALFKAAGVTPLQGHGKLLAGRRVQFTAHDGTQRELSAKHVVLGSGSAPMELRS
                     APFDGKRIVDSWGALEFDAVPKRLGVIGAGVIGLELGSVWRRL"
BASE COUNT         1841 a         3274 c         3447 g         1842 t
ORIGIN      
        1 ccgctccgag cacggtgttg tgatagccgg tcgtgttgta gtaaagcgcc tggtcgcccg
       61 ccgccgtgtt gctgttgccg ttgctgttcg aaaagagcgc ctggaagccg gctacggtat
      121 tgtggttacc gagcgtgttg ccatacagcg cctgatatcc actggcagtg ttctgatagc
      181 ccgtggtgtt ggcatacatc gcttgcgagc cggaggccgt gttgtaggca cccgaggtat
      241 cggtgaagag cgtctggtag ccaactgccg tgttctcgat ggagagggtg ctcgaggtca
      301 gggcgtaggc gccgagcgcg gtgttgtccg cgccgctcgt attgtggaac agtgcgttca
      361 gcccgatgcc agtattgtag aaggcgctga gattctcgtg gaccgctccg gacccgaacg
      421 cggtgttgta gtaaccgccg ctgttatgct gcagcgcctc gtcaccgacc gcggtgttgt
      481 tgtgccccgt ctcgttgaac tccatggcga gaaagcccat cgcaacgttg ccaccgcccg
      541 cagtatcgta atagagcgct tcgtaaccgg cggcggtgtt ctcgatgccg cagtaaaggc
      601 aggaattcgg agtgacgctc tgcagggcgc ctgtgcccat ggcggtgttc tcgttgccgt
      661 cactcacgac gacatcggga ggttgttggg cgagcgccaa cgctgagctg atggcggcgc
      721 agatggccag aatggtggtg gtcgcttggg gggctttcat gggggcacct cgtgaagcct
      781 tgtagagttt tgttttgata tacggcacga agaggcagct ggattgtccg cccggggagc
      841 cggtgaatat gtgaagcagc tcacatcggc atggttatgt acaattgatt acaagtgtca
      901 gcgacgcgtg cgcacgcaga aaggcgcgcg tagccatgga aacaggagca gctcgctgtt
      961 gagcgaggga atcaactcga gccccttggt attgccgtag ttgtcccgcg cgacgtcctt
     1021 gggacgctcc tgccagctct ggtcatagcg gatttcctgc tccaggcgcg gcgtgaccgt
     1081 gaccagcggt gtcatccatt ggggctgcgc ctcttttgcc gcatcggacg tcgccagcca
     1141 atccgctgcg aagctgccgg tgccggaact atcggggccc gcagtggacg ggtctaccag
     1201 ggccggggcc acgagcccca ggagggagcc caaaagcgtg aggggcgcga gcaggtcaag
     1261 gcgcgtatca gtcctcataa gctccccagg acggccgtgc cgcgttatat ctcattgaat
     1321 ataccgatgc gcgcgacttg gtgcgagcgg gagaaattgc gggcgggagg tcgcgggagc
     1381 gagaccagga tcgatcgcct ggagtcaggc gccgcgggtg acttctccgg gtgagtcgcg
     1441 gcgcagcccg atccgtcacg tgaccggttg acctgcgcgc cggcctgcgc gctaatcacg
     1501 cattcctgtg cagcctggtg gagcgttcat ggcggaagac ggtacgaacc tgatcagcat
     1561 gccggcggcg aactcgccca acgcgttcga cctgcgccgg gtcggggcgc acccggacca
     1621 ttggtacgcg ctcgcctggt ccgatgagct gaagagcggc agcgtgctgg gacgccgctt
     1681 cgccgatctg cccattgcac tctatcgggg caaggggggg cgggtattcg cgctcgaaga
     1741 tcgttgcgcg caccggcagg tgccgctgca cctcggggtg gtgagcggcg atcacatcaa
     1801 gtgccactac cacggttgga cctacgatgg cagcggcaag tgcatcgaca ttccctacct
     1861 tggaaaagag cggctgccca acggagtgag gagctatccg gcgcgggagg tggatggatt
     1921 gatcttcgtc tttccgggcg atccggcgct ggccgaggct cgtctgccac gcactctggg
     1981 ctcctcgcag cgcaaggaat acaagacgcg gcggctgaac cgcgaggtgg cctgccacta
     2041 caccttcatg cacgagaatc tgttcgacat gaaccaccag ttcatgcatc gcaagcagat
     2101 gggctcgatc cgcgcgagat gcctcggacg gcagcacggc gagaactggg ccgaggtcga
     2161 gtacactttc acccgcacgg aaggcaagca gtcggtgggc gaacaggtca tcgtggacct
     2221 ggtgcgccgg cggggacagt cgaagcagga tttctccgat cacatgcgaa tccgcaccga
     2281 ctatcccacc cagagcctga aggtctgggt cggccgcaac atcaacgatg ctcaggaccc
     2341 cgtgctcgat gtctggctga gttacacgcc gctggatgct cagcagcgca ccaatcgcac
     2401 cttcggctac ctgtcggtcc gaaagccgcc gattcccgga ttgatccaca tcgtctggcc
     2461 gttcgtgacc tggttcaccg agcggatctt tgctgaggac aaggacatcg tcgagcacga
     2521 gcagagggcc tacgacagtc agggtgcgga ctggaacaac gaggtgtttc ccgcaattcg
     2581 cgacctgcgc gctgtgctgg cgcgctgcgg ggcgcccatc cacgactgaa acggcgcact
     2641 cgagctcgcc cgctacgcgg gtgagcgggc gagcagctca caggtcatgg cgtgctcgga
     2701 gtgcaagggc accaccgcac gcgcgaccag cgactgcgcc ggcaggatcc gctcggcgca
     2761 tacggccgct agatgctcgg gacagggcct cagaagccac aacctccagc gatccgcagc
     2821 atcatttgat tgtggctcga tctgcagcga aagctcatgg tcggcgatat ggaagctgaa
     2881 ctgatcgagc gggatcgaca gacccaggcc ccgcgccttg atgtacgact ccttgagcgt
     2941 ccagtactcg aagaaccgct gctgctgccg cgcggcttcc tgctcgtgca aaccacgcgt
     3001 ctcgatcgct gagaaatagc gctgcgccag ttgcaacacc ggtgtgctgc ggcggacttt
     3061 ttccacatcg accccgaggg cgtaccgatt ggccacaccg aggagcacga cctcctcggt
     3121 gtgcgagatg ttgaatgaca gatccgccgt gtcgggacgc tcgttgagaa ttcgcggccg
     3181 gccgtattca tcgcttgcga agagccaatc ctcgggcgcg acggctgcat aacgcgacag
     3241 ggtcgtgcgc accagggctc gagtgagcag ataccggtga cggccgcgcg gctgaagcag
     3301 ccgtttttcc tgctcccgct cctgcgcact cagcagctcc cgataacgcg caagcaacgt
     3361 gcgatcgcgg atgcgatcgc agaacacgaa ccagagatga atctcctcag gagccgggag
     3421 cggcatgacg gatgtcacga tcacaggttt agtcaggcag cgtgaataac agaccgcggt
     3481 gcccgcgcgc aagatccggg tggccgcccg gctacatcgc cgtcaaagtg ccacttgact
     3541 cgattgttac tgcaagcccg gagccacttg caaccgctcg gcggattcgt tggaaagcgg
     3601 gctattccag acgctacgct ctgagatcgc agcaagacga gaggatgccg cgttgcaacg
     3661 ccccgtggtg ctactgttgg ttacgctggc atttgcaggc acgagcaagg agtgcgccgc
     3721 cgccggcagc gacaactcca tccccgagca gcctgcacct gccgactcaa cctcgagccg
     3781 gcaagcgcgc gatgcggcct ggtggacggg cccgttgctg gcggcctcgg caggcacgtt
     3841 gccgcaggga cattttctca tcgagcccta tgtgttcgat tcgatgcccc aggctcacta
     3901 tgacccgcac ggcgaccgcc agccgctcgc acatgccaac aatttcggtt cgcagagcta
     3961 cgtcctgtac ggtttcacgg agcgcatcag cgcagggctg atcccgagat tcggcttcaa
     4021 cgatccggga acggcgcgcc ctgcggcgca cctcggcgtt ggtgatctcg gcctgcaggc
     4081 gcaatatggc ctgacacgat tcgaggaggg gagttggata cccaccacgt cgctggttct
     4141 aggcgagacg ttacccaccg gccagtacga tcgactcggt gatcgaccgg ccgatggtgt
     4201 gggttcgggt gtctacacca cgacgctggg catctactcg caatacgtct tctggctgcc
     4261 gaccggacgg atcctgcgca ctcgactcga cctctcgtat gcgattccgg gctccgccag
     4321 cgtacaggac gtgagcgtct acgggacctc gcagggattt cgcggtcgcg ccgctcccgg
     4381 caacacgttc atcgcggatc tggcggggga gtacagcctg acgcgcagct gggtgctggc
     4441 gctcgacatc gcttacgagc acgacgcgag cacgcagctc aatgggctgc agcttgcggc
     4501 agcggcgagc ggatcgggat cccccgtcgg gctcccgccc gccgcgatcg cgctcagctc
     4561 cggacccagc cactcgctca gcctggcgcc tgcggtggaa ttcaacttca acagtcgcgt
     4621 gggcgtgatc gccggggcga agctcaccgc ctcgggccgg aacatcaact cggcagtgat
     4681 tccggtcgcg gcagtcaaca tcgtgctgtg agccctgcaa ccagggctca ctttcgggcg
     4741 ttgagtgctg gagacctgca ggcatctcgc cgcttgacta aatgtgtcgc ggtgccgcga
     4801 gtcggcgggt gtcggatgtg atgcggatca cgccgatctg gagctcgggc gcgactgccc
     4861 ccggtagaat agctccgagg ggggtgcgcc tggccgacgg gctcgcgatc cagcagcagc
     4921 cggcggaagg agcggtgagc ataaaatgaa tggtgaagtc ggcggcgatg ccgtgcgagc
     4981 tacgcggcgc gccgtgactc aaagccccgc cgtgagccag gctcacgccg cggaccgtcg
     5041 cgggtccgac cgccgtcaac gcaccctaca cgcgctcatc tatggcagtt tccatccgcg
     5101 ccgtcgccag ccacggcggg cgggcgaggc catcctgagc ggcatcgact ggcatgcgcc
     5161 ccagtggctg gcgatcgccg tgctcattgt cgtgctctcg tgtgtcgatg ccgccctgac
     5221 cctggcgctc ctgcagctcg gggcctacga ggtcaatccg gtgatggccg cgctggtcgg
     5281 gagctcggcg gcggccttca cggccgtgaa ggtcggtctg accggcggcg gggtggtgct
     5341 gctcaccctg ctggcgcgaa tgcgggcctt tggaaagatc ccggtcggcc tcattctgta
     5401 cctggtgctc ctgggctacg gcgtactggt catgtacgag gtccatctgc tggatcagac
     5461 gccgcggcca ttttgagccg ggcccgtctg cctcagccgc ctgcctgcgc gccggcagct
     5521 catttcccgt ggcggaagtg ttccgttaaa ctaaggcctc gctgcattcc ttccccgcat
     5581 gctcgaaccc acacctctgt acggcggcaa cgccgattac ctcgatgccc tctacgagca
     5641 atatctgcgt gatccgggca gcgtggagga ggcctggcgc cactatttct cgcaattgag
     5701 cccggccacg gcggccgagc gagcccacgg gccgattcgc gcggcgattg ccgagcgggc
     5761 ccgggcaccg cgagccgctc aggttgcccg ggaggggggc gccgatgggg ggaatgcccg
     5821 ccaggcggcc gtttcgcgcc tgatccagat ctggacgaat cgcggacacc tggtggccaa
     5881 ggtggacccg ctcgagctca cccagcggcc gcgtccccgc gtgctggagc tggggtattt
     5941 cggcctcacc gaagctgatc tggacacgga gttcttcacg ggcagccgca ccgacgcggt
     6001 gccgaagcgg atgaagctga gggagattct cgcccagctc gagaacatct atggcgggcc
     6061 gatcggcgcc gaattcgccc acgtctcgga gtccgacgag cggctgtggc tgcaggacca
     6121 gttccaggcg ggccgtctgc tgcagcgttt cacaccggag gagcggcgca atctgctgtg
     6181 gcagctgacc gcagccgagg ggctcgagcg ctacctgcat accaagtacg tcgggcaaaa
     6241 gcgcttctcg ctcgagggcg gagaatcctt cgtggcgctg ctcgatgatc tgatccagag
     6301 cagcggggcc gccggggtcg aggaggtcgt gatcggcatg gcccatcgcg ggcggctgaa
     6361 cgtgctggtc aatatcctgg gcaaatctcc ttcggtgatg ttctccgaat tcgagggcgt
     6421 ctatgacctg agccacctca agggctcggg cgatgtgaag tatcacaagg gcttctcctc
     6481 tgatctgcgc acgcccagcg gcaacgtcca taccgttctc gccttcaatc cctcgcacct
     6541 cgaagtggtc gatccggtgg tcgaaggctc ggtgcgggcg cgccaggagc gcagggggga
     6601 tgcgagcggt gaccgggtgt tgcccgtgct ggtgcacggg gatgccgcct tcgcgggcca
     6661 aggggtggtg atggagaccc tgcagctcgc gcaggcccgc ggctattaca ccggcggcac
     6721 cctccacctc gtcatcaaca atcaggtcgg cttcacgacc tccgatcccc gcgatacccg
     6781 ctccacgatc tactgcagtg acgtggcgaa gatgctggaa gcacccattt ttcacgtcaa
     6841 tgccgatgat ccggaggccg tggtgttcgt aacgcggctt gccctcaaat atcgcctgaa
     6901 attccacaag gacgtggtca tcgacctggt ttgttaccgt cgccatggcc acaacgaggc
     6961 cgacgagccc gcagcgaccc agcccgtcat gtaccgggcg attcgccagc atcccacggc
     7021 acgcaaactc tacggcgaca ggctcgtcgc ggagggtgtg atctcggagc ccgaggtcgc
     7081 ccagatgatg gagcagtacc gcgcggggct tgacgagggc aggccgcagg cacgtgcctc
     7141 cctcggaatg atcggtaaca agttcaccgt ggactggcgc ccgtattcgc agatcgactg
     7201 ggcggaacga atccagacgg gggtggagct tgcgcggttg cgttctctgg gtgagcggct
     7261 cacccgcttt ccggaggact tgagcctgca ccctcgggtt gcccagatca tcgccaatcg
     7321 ccgccggatg ctcgccggcg agcatccgct ggactggggc tatgccgaga cgctggccta
     7381 cggaagcctg atcgaacagg gctatgccgt tcgtatttcc gggcaggaca gcgggcgcgg
     7441 caccttcttc caccgacacg cggtgctgca cgatcagaac agtgatcgca cgtacatccc
     7501 gctgcagcat ctggccgagc gccagccgcg agtgcaggtg atcgactcgg tgttgtcgga
     7561 agaggccgtg ctcggtttcg agtacggcta ctcgaccact gaacctaaca cgctggtggt
     7621 gtgggaagcg caatacggcg acttcgccaa cggggcgcag gtgatcatcg accagttcat
     7681 cagctccggg gaggccaaat gggagcgctt ctgcggcctg gtgctgttcc tgccgcacgg
     7741 ctacgaaggc gcaggccccg agcactcctc ggcgcggctc gagcgcttcc tgcaactgtg
     7801 cgccgaatcc aacatgcagg tttgtgtgcc ctccaccccg gcgcagatgt tccacatgct
     7861 gcgccgacag atgctgcagc ctttccgcaa gccgctgatc gtcatgaccc cgaagagcct
     7921 gctgcgacat gagctgtcgg tgtcggggct ggaagatctc gcgcgcggca gcttcgctcg
     7981 tgtcatcgac gaagccgatg acctgccgcc cgcgcaggtg cgccgcctgg tgttctgcag
     8041 cggcaaggtc tacttcgatt tgctgaaggc acggcgcaag gacggcattc gcgatgtggc
     8101 gctcgtgcgc atcgagcagc tctacccgtt cccgagtgag gagtacgagg cgatcctgaa
     8161 tcgatactcg aacgtgcgcg agatcgtctg gtgccaggag gagccccaga atcagggcgc
     8221 gtggtatcag atccgccacc ggctgcagga attgctcggc ggtcgcgcgg tactttacgc
     8281 gggccgggcg ccagcggccg caccggccac gggcattgcg aagatccacg aggctgagca
     8341 gcaggcgctg attcacgccg ccttgcaggc gaccgctacc gaagactcgg cgcgcgaaac
     8401 cacccggctc acgaacgcgg caccggcggc cgcacccaag gtcagctcgc caactccggt
     8461 gcccgctttg agaaagagct catgacgact gaaattcgcg tgccgcagct tcccgaatcc
     8521 gtcgcggatg ccacgctcgt cgcctggcat aaacagccgg gcgatgcggt gcgacgtgat
     8581 gagaatctgg ccgacctcga gaccgacaag gtggtcctcg aggtcccggc accgctcaat
     8641 ggtgtggtgc gggagatccg gattccgagc ggcaccacag tcaccagcgg tgaggtgctg
     8701 gcgctgattg atgaaggggc gcagccggca gcgagcgcag gcgcacctgc cgcggccaaa
     8761 gcgccggcct caccctctca ggcttcgccc gctccgcagg cttctgcgcc caagcctgcc
     8821 tccacagcga aagaaaccga caacggcagc gcacgggatc tcggcaaact gagtccctcc
     8881 gtgcggcgcc tggtcgagga aaaccgactc gatccggact ccattcctgc ctcgggcaag
     8941 gacggacggc tgaccaaggc ggacgtcgtg gagtttttgg agaagcggcc cgcgaccgcg
     9001 tcgcccgaac cgacagcatc gcctgccacg ctgagcccac cggccgcggc gcgagcggat
     9061 gcccctggcg ccaagccggc ttcgggggcg cgcgcgcagc aacgagttcc gatgacaagg
     9121 ctgcggcagc gcattgcgca gcggctcgtc gaggctcagg ccacccaggc gttgctcacc
     9181 tcattcaacg aggtggatct gactgccgtg caggatctgc gggcgcgcta caaggatcga
     9241 ttcgagaagg agcacggcat caagctgggg ttcatggcct ttttcgtgaa ggcctcgatc
     9301 gaggcactca agaaattccc catcatcaat gcttcggtgg acggcaccga catcctctat
     9361 cacgagtatt acgacatcgg cgtcgcggtc tcgaccgacc gcgggctgat ggtgccgatc
     9421 gtgcgcgatg cggatgcgaa gagcttcgcc accatcgaga aggagatcgc tcagtttgcc
     9481 cgcaaggcgc gcgaaggctc gatcggtatc gaggacctca ccggcggcac tttcacgatc
     9541 accaatggcg gggtgttcgg ctcgctaatg tccacgccta ttgtaaatgc gccgcaaagc
     9601 gccattctgg gtatgcacaa gacccaggag cggcccatgg tcgtgaacgg acagatcgcc
     9661 atccgtccga tgatgtacct cgccgtcacc tacgatcacc ggatcatcga tggccgcgag
     9721 gcggtgcagt tcctggtggc ggtgaaggaa ggcctggaag atccgggccg gatgttactg
     9781 gggatctgag cctgcatgag tgattcattc gatgtcgtcg tgatcggcgg cggcccggcc
     9841 ggctaccctg ccgcgattcg cgccgcgcag aacaaactca gcgtggcctg catcgacgag
     9901 tggaagaacc acgacggcgg tgcggccttc gggggcacct gcctcaatgc cggatgtatt
     9961 ccctccaagg cgctgctcga gtcctcggag ctgtatcacc gtgtgcgcga ggagttcgcc
    10021 gtccacggga tccggaccac cggggtttca ctcgagctgg cccagatgca aaagcgcaga
    10081 gccggcatcg tgcgcaccat gacgcagggg atcctggctc tcttcaaggc ggcaggcgtc
    10141 acgcctctgc aggggcacgg caagctgctt gcggggcggc gcgtgcaatt caccgctcac
    10201 gatggcacgc aacgcgagct gagcgcaaaa catgtcgtgc tcggctcggg ctcggcgccc
    10261 atggagctgc gctcggcgcc tttcgatggc aagcgcatcg tggattcctg gggcgctctg
    10321 gagttcgatg ccgtcccgaa gcgcctcgga gtgatcgggg ccggagtgat tggcctggag
    10381 ctcggaagcg tctggcggcg cctc
//
LOCUS       DASRHN010000169         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_55043, whole genome shotgun sequence.
ACCESSION   DASRHN010000169 DASRHN010000000
VERSION     DASRHN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55043"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VFB37_06615"
     CDS             complement(<1..1259)
                     /locus_tag="VFB37_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZO22162.1"
                     /translation="MSSGIAALAKSRSASVNVRRDVPQQAAAPDPKLADPERLKPFPL
                     TDTQEAYWLGRQSAFELGGTSLHSYRESEIENLDLERLNDAWLMLVQRHEALRTVVTE
                     SGTQRLVGDIPPYRIALTDLSGADSAAVERHLQTTRQEMSHRLFPLEVWPHWDIRATQ
                     LSGQRTRLHISFDGILIDDRSYQILIREWFKLYRSPTTRLPPLGFSFREYVLAERAQR
                     DSPPYQTDLRYWQQRITTLPAAAELPLLPGPKPSGNTRFTRYQDRLDPLAWAQLKKLC
                     ASCQITTAGLLLSCFMEVLSRWSKSSELTVSVPTLNRPPFHADINDVVGDFGSFILVG
                     NDGATDGTFLDRIRTVQQRLWEGLEHDRVSGVRILRELMRHKKAVAAVQMPIVFTMAS
                     SLKDPQRTDQPPTEQRSTAGGAQAESVA"
     gene            complement(1298..>2398)
                     /locus_tag="VFB37_06620"
     CDS             complement(1298..>2398)
                     /locus_tag="VFB37_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HZO22163.1"
                     /translation="EDFLRGVMRRRTQRRFEPGPIGLAQFSGLLSVLMQVMPEGFAMP
                     KYQYPSAGNAYPLQFYVLVRAGRVQGVSEGVYYYHPRDHRLIQLSPDGNWEDTLHPAN
                     NVPVSQEAAFSLFVICEMDAMRPLYGKMARDFGLLEAGYVSQLLMSRAPELNLGLCPL
                     GTMDFEPLRARLALRESHVLFHGFLGGAIPQAELSDPRLYLTDASKKWSQQDEAPGAG
                     EFAEFLRKRLPRHMIPDRFAVLDRLPLTTNGKVDRAALQSMEEQVVSLGGYVEPQTPL
                     QKALAGLWSEVLGVERTGLRDDFFQLGGDSILAIQLMNRLKSSFSTDIALRDVLAATT
                     VEQLAEVLERRLAADLEREALAAGPPGAGTRE"
BASE COUNT          452 a          785 c          731 g          430 t
ORIGIN      
        1 gcgacggact ccgcttgcgc gccgcctgcg gtcgaccgtt gctcggtcgg cggttgatcg
       61 gtccgctgtg gatctttcag gctggacgcc atcgtgaaca cgatcggcat ctgtacggca
      121 gcgacggcct tcttgtgcct catcaactcg cgcagaattc tcacaccgct cacacggtcg
      181 tgctccaacc cttcccaaag gcgttgctgg acagtgcgga tgcgatccag gaacgtccca
      241 tcagtcgccc cgtcgttgcc gaccaggatg aaggagccga aatcgcccac gacatcgttg
      301 atatccgcat ggaacggcgg tcgattgagc gtgggtacgc tgactgtcag ctccgacgac
      361 ttactccatc gagacaggac ttccatgaaa caggagagca gcagcccagc cgtcgtgatc
      421 tgacagctcg cgcagagctt cttcagttgt gcccacgcga gcgggtcgag ccggtcctgg
      481 tagcgtgtga agcgtgtatt gcccgagggt ttcgggcccg gcagcagcgg cagctccgct
      541 gcggcgggca aggtcgtaat gcgttgctgc cagtaccgca ggtccgtctg atacggcgga
      601 gagtcgcgtt gcgcacgctc ggcaagcacg tactcccgga aagaaaagcc gagcggcggc
      661 aaccgcgttg ttgggctcct gtagagcttg aaccattcgc gaatgagaat ctgatagctg
      721 cgatcatcga tcaggattcc gtcgaagctg atgtgcagcc gggtgcgctg tccgctcagc
      781 tgggtcgcgc gtatgtccca gtgcggccag acctccagcg gaaacaggcg gtgcgacatc
      841 tcctgacgag ttgtctgcag atgtcgttcg acagcggcac tgtcggcgcc gctgagatcg
      901 gtcagggcaa tgcgatacgg cggaatgtcc cccacgagtc tctgggtccc tgattcggtg
      961 acgaccgtgc gcaaggcctc atggcgctgc acgagcatga gccacgcatc gttcaggcgc
     1021 tccagatcga gattctcgat ttcactctcg cgatagctgt gtagcgacgt gccacccagc
     1081 tcgaatgcgc tctgccggcc aagccaatag gcttcctgcg tatccgtcag aggaaacggc
     1141 ttcagccgct cgggatcggc aagcttcggg tctggcgcgg cagcctgctg gggcacgtcc
     1201 cgccgcacgt tgactgaggc agagcggctt ttcgccaggg cggcgattcc gctgctcaac
     1261 ctatcctcca acagccggcg attgtccatt ggcagaccta ctcccgcgtc cccgcgcccg
     1321 ggggacccgc tgccaacgct tcccgctcga gatccgccgc caggcggcgt tcgaggacct
     1381 cggcaagttg ctccaccgtc gtcgcggcga ggacgtcgcg gagcgcgatg tcggtggaga
     1441 acgaactctt caggcgattc atcaactgga tcgccagaat cgagtcgccg ccgagctgga
     1501 agaagtcatc ccgcaacccg gtccgctcga cgccgagcac ttccgaccac agaccggcaa
     1561 gggctttctg caagggcgtc tgcggctcca catagccgcc cagcgagacg acctgctcct
     1621 ccatcgattg cagcgccgcg cgatccactt tgccattggt cgtgagcggt aagcggtcga
     1681 gcaccgcgaa gcgatccgga atcatgtggc gaggcagccg cttccgcagg aactccgcga
     1741 attctccggc gccaggggcc tcgtcctgtt gcgaccattt cttggaagca tccgtcaggt
     1801 acaacctggg gtcgctgagc tccgcctgcg gtatcgctcc tccaaggaag ccgtgaaaca
     1861 gcacgtggct ctcacgcagc gccaggcgcg cccgcagggg ctcgaaatcc attgttccca
     1921 ggggacacaa acccagattc agctcggggg cacgcgacat cagcagctgg ctgacatagc
     1981 ctgcttccaa cagaccgaag tccctggcca tcttcccgta caacggacgc atcgcgtcca
     2041 tctcgcagat cacgaacagg gagaacgcag cttcctgaga gaccggcaca ttgttagccg
     2101 gatgcagcgt gtcctcccag ttgccgtccg gggacaactg aatcaagcgg tggtcgcgcg
     2161 ggtgatagta gtaaacccct tcgctcacac cctgcaccct gccggcacgc accaacacgt
     2221 agaactgcag cggatacgcg ttgcccgcgg acgggtactg gtacttgggc atggcgaagc
     2281 cctcgggcat cacctgcatc agcacgctca gcaacccgct gaactgcgcc agaccgatcg
     2341 gtccgggctc gaaacgtcgc tgagtacgac gccgcatcac gccccgcaga aagtcctc
//
LOCUS       DASRHN010000170         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1703905, whole genome shotgun sequence.
ACCESSION   DASRHN010000170 DASRHN010000000
VERSION     DASRHN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703905"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFB37_06625"
     CDS             <1..664
                     /locus_tag="VFB37_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZO22164.1"
                     /translation="RLPPGSNHWFQLMPLIRKPAETRPPSKPDGSSVLKALASAHQEE
                     RWAAARAAAQVPGGVAALAAALRTEHDPRVREAMFSGLAGAGTPEGIQEIIAFLRSDN
                     ANLRTGALDALRAAAGAVHTVVPQLLHDADPDIRILSCELARSLPSVEAASLLCELIG
                     REQQLNVCAAAIDVLAEVGSSDALPALAACAQRFRDAPFLAFAVRIAIDRIHSQSTLG
                     RD"
     gene            657..1511
                     /locus_tag="VFB37_06630"
     CDS             657..1511
                     /locus_tag="VFB37_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZO22165.1"
                     /translation="MTELPALTEEEFRRLCEYLYRRTGMVFTEAKRYYVERRVIERIS
                     ATGSGSFASYFGRLRSDVQNEIELFVNAFTVNETYFYREEHQLECLTSDLLNQRVLDK
                     RTGDALRIWSAPCSTGEEPYSLAIWLLENWPRVDEYEIEIIGSDIDTEVLELARAGIF
                     GKRALMRLSPYLIGKYFESLSEESWRILQDLRDSVRFSPVNLVESAETRAHGQFDVIF
                     CRNVLIYFDDAARRVAAENLYEALLPGGFICLGHTESMSRISPLFEVRRFPDAIVYQR
                     PDGVARDR"
     gene            1501..1887
                     /locus_tag="VFB37_06635"
     CDS             1501..1887
                     /locus_tag="VFB37_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22166.1"
                     /translation="MTDSQKTVMVVDDASLVRLYYRTALEGAGFRVEEALNGIEALEK
                     LLSIPVDLLIVDVNMPQMDGFTFLDVLRQKTGPLASVPALVTSTESAEADFAAARNAG
                     ANYYLVKPIEPQTLVEFASMFCGIVR"
     gene            1884..>3583
                     /locus_tag="VFB37_06640"
     CDS             1884..>3583
                     /locus_tag="VFB37_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZO22167.1"
                     /translation="MNDFLQQFLIESRELVEQASYGLLKLEHTPTDAPELDAVFRAFH
                     TLKGGAGIVEFAAMERAVHAAEDVLTEARAGKRVLTPAAIGACLACLDQVAQWLGVLE
                     QRGELPSAEAVGEAEKIVHRFEIARGGTRAEVSDTHEAAKPDWVTAALDRSGRSGEAS
                     TAVRFVPEPECFFQGEDPIARMISLPQLLALAVEPVQAWPLLASLDPLTCNLALMALT
                     AASVGHVRAHMQGASGECETVALAPEVAVTAGSELPPRVREVLEAQRALIANGKPETL
                     AGRLASAGLTATNVLRFSGRLDQADAVAGATRTSLAQQVFQPLRDAINQALAAHTPLP
                     LAAEETVRAATAAPRTLRVDAQRIDTLVRLTGELTVAKNSLHHLSKLARTDGNTLAGA
                     LKDCHGVFEHLVGELRRSVLAMRVLPLRSVIQRFPRVLREMSASLGKPARLQTEGEDT
                     EADKAIVEMLFEPLLHVVRNALDHGVETPAERSARGKPPVATIRIRAARQGDQVHIDV
                     CDDGGGIDVERVRRIARDRGVASDEELRSMPETGLINLVFAAGFSTAEKVTEFSGRGV
                     G"
BASE COUNT          644 a         1107 c         1154 g          678 t
ORIGIN      
        1 aagattgccg cccggctcca atcactggtt ccaactcatg ccactcatcc gtaagccggc
       61 ggagacccgc ccaccctcca agccggacgg ctccagcgtc ctgaaggccc tcgccagcgc
      121 ccaccaggag gaacgttggg cggctgcccg cgccgccgcg caggtccccg gaggggtggc
      181 tgcattagcc gcagcgctgc gcaccgaaca cgacccgcgc gttcgggaag ccatgttctc
      241 ggggctcgcc ggcgccggga cgcctgaagg catccaggag atcatcgcgt tcctgcgctc
      301 cgacaatgcc aatctgcgta ccggagcgct ggatgcgctc cgagccgcgg ccggagcggt
      361 ccatacggtt gttccgcaat tgctgcacga cgccgatccg gacattcgca tcctcagctg
      421 tgagctggct cgaagcctgc cgagcgtgga ggcggcctcg ttgctgtgcg agctcatcgg
      481 ccgagagcag cagctcaatg tgtgcgctgc agccatcgat gtgctcgccg aagtgggcag
      541 ctcggacgcc ctgcccgccc tcgccgcatg tgcacagcga tttcgcgatg caccgtttct
      601 cgccttcgca gtcaggatcg ccatcgatcg catccactct cagtcaacgc tcggccgtga
      661 ctgagcttcc cgctctcacc gaagaggagt ttcggcggct ttgcgagtat ctctatcggc
      721 gcaccggtat ggtgttcacc gaggcgaaga gatactacgt ggagcgtcgt gtgatcgagc
      781 ggatttcagc caccggctcc ggatcctttg cgagctactt cggccggctg cgttccgatg
      841 tacagaacga aatcgagctg ttcgtcaacg ctttcaccgt caacgagacc tatttctatc
      901 gcgaagagca ccagctggaa tgtctcacga gcgatctgct caatcaaagg gttctcgaca
      961 agagaaccgg cgacgcgctt cgcatctggt cggccccctg ctccaccggt gaggagccct
     1021 actccctggc catctggctg ctggaaaact ggccccgagt cgatgaatat gagatcgaga
     1081 tcatcggctc ggacatcgat actgaagtgc tggagctggc tcgtgcaggt atcttcggca
     1141 agcgcgcgct gatgcgtctg tcgccgtatc tcattggaaa atacttcgaa tcgctgagcg
     1201 aggaaagctg gaggattctg caggacctgc gcgattcggt ccgcttctcg cccgtcaatc
     1261 tcgtcgagtc tgccgagacc cgagcgcatg gtcagttcga tgtcatcttt tgcaggaatg
     1321 tcctgatcta ctttgacgac gcggcgcgac gggtggccgc cgagaacctc tacgaagctc
     1381 ttctgcccgg aggcttcatc tgcctcgggc ataccgaatc catgagtcgg atatcgccgc
     1441 tgttcgaggt caggcgcttt cccgatgcca tcgtttatca acgtccggac ggggtggcac
     1501 gtgaccgata gtcagaagac ggtcatggtc gtggacgatg ccagcctggt gcgcctgtac
     1561 taccggacgg cgctcgaggg ggccggcttt cgagtcgagg aagcgttgaa cggcatcgag
     1621 gcgctggaga agctgctgag cataccggtc gacttgctga tcgttgatgt caacatgccg
     1681 cagatggatg ggtttacctt tctggatgtg ctgcgccaga aaaccggccc tctcgcctcg
     1741 gttccggccc tggtcaccag cacggaatcg gcagaagcgg acttcgcagc ggcgcgcaac
     1801 gccggggcca actactacct cgtgaagccg atcgagccgc agacgctagt ggaattcgcc
     1861 tctatgttct gcggtattgt gcgatgaacg attttctgca gcaattcctg atcgaatccc
     1921 gcgagctcgt cgaacaggcg agctacggac tgttgaagct cgagcacact cccacggacg
     1981 cgccggagct ggacgctgtc ttccgcgcct ttcatacact gaaaggtggc gctggaatcg
     2041 tggagttcgc cgcgatggag cgcgccgtcc acgccgccga ggatgtcctg accgaagctc
     2101 gcgccggaaa gcgcgtgctg accccggccg cgatcggcgc ttgtctcgct tgcctcgacc
     2161 aggtagcaca gtggcttggc gtgctcgagc aaagaggcga attgccctcc gccgaggccg
     2221 ttggagaggc cgagaagatc gtccatcgct tcgaaatcgc ccgcggcggg acacgagccg
     2281 aagtttccga tacgcatgag gcagcgaagc cggattgggt gaccgcagct ctcgatcgca
     2341 gtgggcgctc gggcgaggcg agcacggcgg tcagattcgt gccggagcct gagtgtttct
     2401 ttcagggtga agatcccatt gcgcgcatga tttccttgcc gcagctgctg gcgctcgcag
     2461 tcgagcctgt ccaggcgtgg ccgctcctgg cgagcctcga tcccttgacg tgtaacctcg
     2521 cactgatggc actgacggcc gcatcggtcg ggcacgtgcg ggctcacatg caaggagctt
     2581 cgggggagtg cgagacggtt gctctggcgc cggaggtcgc tgtgacggcc gggtcggaat
     2641 tgccgccgcg cgtgcgcgag gtgctcgagg cgcagcgagc cctcattgca aacggaaagc
     2701 ccgaaacgct ggcggggcgc ctcgcctccg cgggattgac tgcgaccaat gtcctgcgct
     2761 tcagcggccg tctggatcag gctgatgccg tcgcgggtgc gactcgaacg agccttgccc
     2821 agcaggtctt tcagccgttg cgggatgcta tcaaccaggc actggcggcg cacacgccgc
     2881 taccgctcgc cgccgaggaa acagtccgcg cagcaaccgc ggcgccgcgt actctgcggg
     2941 tcgatgcgca gcgcatcgac actctggtgc gactcacagg ggagctgact gtagcgaaaa
     3001 actcgctgca tcatctgagc aaactcgctc gcacggacgg caatacgctg gccggtgccc
     3061 tcaaggactg tcacggggtc ttcgagcacc tcgtgggcga attgcgaaga tcagtgttgg
     3121 ccatgcgggt gctccccttg cgatccgtca ttcaacgctt tccacgagtg ttgcgcgaaa
     3181 tgtcagccag tctgggcaag cctgcaaggc tacagaccga aggtgaggat accgaggcag
     3241 acaaagccat cgtggagatg ctgtttgagc cgctgctgca tgtggtacgt aatgctctcg
     3301 accacggcgt tgaaactccg gcagagcgca gtgctcgcgg caagccccct gtggcgacga
     3361 tccgcatccg ggcggcgcgc cagggggacc aggttcatat cgacgtgtgt gatgacgggg
     3421 gcggcatcga tgttgagcgc gtccgcagaa tcgcgaggga tcgcggcgtg gcgagcgatg
     3481 aggaactgcg ctcgatgccg gagacgggcc tcatcaatct ggtgttcgca gcaggcttct
     3541 cgacagccga gaaggtcacc gagttttccg ggcgcggtgt ggg
//
LOCUS       DASRHN010000171        11924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1042977, whole genome shotgun sequence.
ACCESSION   DASRHN010000171 DASRHN010000000
VERSION     DASRHN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042977"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFB37_06645"
     CDS             complement(<1..832)
                     /locus_tag="VFB37_06645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO22168.1"
                     /translation="MPRARHTAMIECLIQGEISADAESDPLEALSLMRRALPPTRALL
                     ALAPGTSRGKLRETLWADLLEIELVSDRREALSRLARDFYPIVLTDSLELIRALRHRR
                     SRCSAVLIYFSARSDSADRAAGIRAGADDCIGNDASEEELHARITAARRVAELESVLR
                     AALAENHRLSSVDDLTRIGSKQFFNRQFTREAERAARSGRALSLILCDIDHFKIINDT
                     LGHASGDEVLRHFGTRLQRLVSGECSWIARVGGEEFAIVLPGVALPEATETARALCLG
                     T"
     gene            1115..2785
                     /locus_tag="VFB37_06650"
     CDS             1115..2785
                     /locus_tag="VFB37_06650"
                     /inference="COORDINATES: protein motif:HMM:NF006002.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZO22169.1"
                     /translation="MAPRELAGTACHHPVVISGAGPVGLAAAACLARFGIRTVVLEKS
                     TRAGEGSRAICLARRSLEILDWIGVAERFVSKGLGWTRGHAFHGAHPIHELCMPHSDA
                     EKFFPMTNLQQYYMEEFLAQHCARQEAIELRWGNELVGIQPEPDGVQLRVATTAGMYQ
                     TSCSWLLAADGARSPARRLLQLPLCGSSFAGRYLIADIRLRSSYPTERRAWFDPPSNP
                     GSTVLMHRQPEDIWRIDYQLQEHDDDRRESEPHRVRERVQRHLDFIGEHGEWELLWTS
                     IYRAHSLALESYVHGHVLFAGDAAHLVPIFGVRGLNSGLADAQNVAWKLAWVLRGDAP
                     ERLLHSYSSERRNATLDIHRQAEKSTRFMTPSSLGERLMRAAVLSLASRHEFVRPLID
                     PRQSVPYTYAGSELNTPEEAAAGLEAFRSGPAPGAPLHNLRIRGHSGPGYLLERLGTG
                     FTVLLFLPGKWTGAHAECLEQLRAVTRLDSRGELRLLVLTGAPPAERDSSEYVELLQD
                     VAGVAPQIYDARPGTVYLARPDGHVCARWRAATVRDVGVGLCRACTVI"
     gene            2795..3001
                     /locus_tag="VFB37_06655"
     CDS             2795..3001
                     /locus_tag="VFB37_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22170.1"
                     /translation="MHSQSGEGPDSSLERAHALLVAAIDSQPPGTEALYLARLALLLL
                     NELDCSERALGLIEAARQPPSTDP"
     gene            complement(3055..4479)
                     /locus_tag="VFB37_06660"
     CDS             complement(3055..4479)
                     /locus_tag="VFB37_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZO22171.1"
                     /translation="MNSSLPSTPVLSVSSQEFRELATRVVELAAQFYGRLEDLPAYPQ
                     TTGMQTLQAFGGPLPAAGMGAAALEALERVVQLSRPPGPRFFGYVLGSGEPVAALGDL
                     LASVINQNLTAWRSAPAGVAIERIVVGWIAEAIGCPHLTGSLCGGGSTANLMALAMAR
                     EARLPANERGARPGTVYVSSEAHMSMAKAVGLLGLGRDNMRIVPVDERLRMQVGALEH
                     LIEADRATGRELVAVVASAGTVSTGAIDPLQAIAAVCERHGLWMHVDGAYGAAAALAC
                     PEKFAGLERADSLSLDLHKWLYQPVDCGLLLFRDPARARAAFAYTGDYARVLEGESIE
                     GLAIFEESLELSRRFRALKVWLSLRYHGVQAFREAIRADLDHAAQLSLRIGECPELEL
                     LAPTDLSAVCFRYRGDGGAQDLDRLNGAILKRVIDNGRVYLSNATVFGKVALRACFVN
                     HRTRAADVAEIVSEVLQAGRAIAT"
     gene            complement(4493..5326)
                     /locus_tag="VFB37_06665"
     CDS             complement(4493..5326)
                     /locus_tag="VFB37_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZO22172.1"
                     /translation="MKHKPGHRSGERGAQPESGDGLQSLQTAFRILDELSDAHGPVGL
                     TDLALRLGELKPRVYRHLSTMKRLGVVFQDPRNGGYSLGGKLFTLGEAALEQFDLRFL
                     AAPYLTRLRDETHQTALLSVPGNGEPIVLSCVEYRNRLSISSRPGNRPPPHCSSQGRI
                     ALAFADPPARERVLGGKLAAFTRHSITDRALIEQRLEGIRERFFEQAVDEVRLGINAV
                     SAPLFRAEDELIGIIGIVGTTPEIGNPPPRSLIATLHGVAAALSAELNCRIYYERGLV
                     K"
     gene            5448..6557
                     /locus_tag="VFB37_06670"
     CDS             5448..6557
                     /locus_tag="VFB37_06670"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZO22173.1"
                     /translation="MFLKNCWYVAALDHELIDGKLLSRMILGEHLLLYRGESGKVIAL
                     NDRCPHRGALLSRGRLEGDAVRCMYHGIKYDGSGKCVQIPGQDMIPPKLRVRAYPVEQ
                     RSHFVWVWMGDPAKADPSLIVDLPYLHEPRWKGLPAYLHYDANYLLIVDNLSDFAHLA
                     FVHTKTLGGSEEYAYVSKPTSIERLERGFRVERWHLNSDPPPFHKKVIPAAEKTSRVD
                     RRNIATMLIPGIFTMETLFAPAGSGAPQGNIGDAKEYRNCQFMTPETERSTHFFWAYL
                     NNYEGQDSTISISLLDSLIEGFMEDKAIIERQQQTLDEDPAFEKQMLAILADAPLAHF
                     RRVLGKLLEAEHSAASGPPSAATTPEGARLTTAAG"
     gene            6632..9049
                     /locus_tag="VFB37_06675"
     CDS             6632..9049
                     /locus_tag="VFB37_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22174.1"
                     /translation="MLTIKRFLAGACVLAVAAPAVQAATPADASTSSTGGEALEEVIV
                     TAQKRAESEQNVPMSITTFGSVQLQEKAINNFFDYATKVPNLAFAPTGDGVGTARTVS
                     IRGISGDNVTGFYIDDTPLPDSIDPRVLDIDHIEVLRGPQGTLYGARSMGGTVRIITK
                     VPDLTGFSATVHAAASDTDHTDEPNYTFDGIANIPILSDRVALRLTGFYDNQAGWFKR
                     SFCTDPSTAGTSCFPLSTTGRTTLDNIAQNDTFGGSASLTIKLSDALTITPRVMMQRA
                     RYNGFPMADFNSMPNNGIGFPAPSGPYTLPQPLVSNDLTQARFFDVPEGGTDKWDLYS
                     VAVHWKTDLGELVSSTAYFDRKVDETEDQSEFIWAAITSGACATPAPYCNPPAPGPIE
                     EIKDYQRFVEEVRFASSLAGPVQFVAGAFYSDFHGAIPFASLYPPSQVPNLDNELTGG
                     FPNNPDGIPNLVFAQNYHTDIEEPAVFGEVSWNLTSALKLTGGLRWYQVKTTSAGTEE
                     GLATGSDTLVVGPRATDKENGTNPKVQLDYHLTPDEMVYALASKGFRPGGVVPIVPPG
                     TPGTANDCVAALAAVNPKLNLADTRSFKSDSLWNYEVGTKTAWLDHRVIFNASAFDIR
                     WKNIQQPILLGCGFQFTSNAGAAESKGGELELRGRATEQLEGSLGVGYQDAKITEKGN
                     SPQPAGSPVFQVPNWTGNASVTYTVPLANGWNAASGLDYSYIGSSFSSSNGPVLPRER
                     PAYRLLDARLAMTNGSTEVALVGKNLTNELTDLGDNRSIAAEVPGRPRLFVNQPRTIG
                     IEVRQSF"
     gene            complement(9051..10286)
                     /locus_tag="VFB37_06680"
     CDS             complement(9051..10286)
                     /locus_tag="VFB37_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22175.1"
                     /translation="MKAPIARLLIVDDESAQMKALCETLSVEGYSTTGFTSAKRALET
                     LRSEEFDLLLTDLMMPEMDGITLLTSARAISPDVMAIVMTGHAAIDTAVKAMQAGALD
                     YIVKPFKLNAMLPVLNRAISMRQLRVANRELEERVRQRTEELESANRQLEVVNRELES
                     FSYSVSHDLRAPLRTVQGFCQMFMEDFGASIPAEGRPLLERVEAGARRMGELIEDLLA
                     LSQLGRRPVLRGTVALEPLVRRLTADLLARDATRRVEVRVGNLGSCHADGSLLEQVLV
                     NLLSNAFKFTRQRDPAIIEIDCLQRGQELVYHVRDNGVGFKMEYAGKLFGVFQRLHSE
                     SEFEGTGVGLSIVQRIIQRHGGRIWAESAVGKGTTFYFTLPPAGAAAAAGPAPTAVEA
                     EPAADSIEAAPAASAASGG"
     gene            complement(10305..>11924)
                     /locus_tag="VFB37_06685"
     CDS             complement(10305..>11924)
                     /locus_tag="VFB37_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22176.1"
                     /translation="GIVLRSLEESLPIDFGCIGLVDGDELKVASLGSSALGSQLGFGS
                     QASIPLDSNGLSRCIRGELVYEPDVPEVPFPFPQRLARAGLRSLVIAPLGIENRVAGV
                     LIAARLRENGFASADCEFLRHLTEHVALASHQMRLYTALQQAYEDLRQTQHTVMQQER
                     LRALGQMASGIAHDINNAISPVSLYTESLLEREPNLSERARNYLVTIQRAIEDVAATV
                     ARMREFYRQREPQLELARIDANRILCHVIDLTKARWSDLPQQRGVVVDLHTELVSGKA
                     ELMGAESEIRDALTNLIFNAVDAMPEGGTLTLRTRLLSSAQSEQQHAEDRVCMEVSDT
                     GIGMDEETRRRCLEPFFTTKGERGTGLGLAMVYGMVQRHSAELELESEPGRGTTFRLI
                     FPAFTETISATVHIPRQVIAQRLRILLVDDDPLLIKSLRDALEGDGHLITATSGGQAG
                     IDAFSAAVAAGPRFDVVITDLGMPYVDGRKVAAAIKAASARTPVIMLTGWGHRLVAEN
                     DVPAHVNRVLNKPPRLQELRGALAELIAEAQ"
BASE COUNT         2121 a         3986 c         3803 g         2014 t
ORIGIN      
        1 cggttccgag gcacagggcc cgcgcggttt ccgtggcttc cgggagcgcg acgcccggca
       61 gcacgatggc gaactcctcg ccgccgacac gcgcaatcca actgcactcc ccgctcacga
      121 gacgctgcag ccgcgtgccg aaatgccgca gcacttcgtc tccgctggcg tgtccgagcg
      181 tgtcattgat gatcttgaag tggtcgatgt cacacagaat gagcgagagt gcccgcccgg
      241 agcgcgcagc tcgctcggcc tcgcgagtga actgccggtt gaagaactgc ttgctgccga
      301 tgcgtgtcag gtcgtccacg ctcgacagac ggtgattctc ggccaacgct gcgcgcaaca
      361 cggattccag ctcggcgacg cgccgcgccg cggtgatgcg tgcgtgcaac tcctcctcgg
      421 aggcgtcatt tccgatgcaa tcatccgccc cggcgcgaat gccggccgcg cggtctgcgc
      481 tgtcggatcg ggccgagaaa tagatgagca ccgccgagca gcgtgacctg cgatggcgca
      541 gggcgcggat gagctccagg ctgtcggtga gcacgatggg ataaaaatcg cgggccagcc
      601 ggctcagggc ctcgcggcgg tcgctgacga gctcgatctc gagcaggtct gcccacaggg
      661 tttcgcggag cttgccgcgc gaggtgccag gggccagtgc gagcagagcg cgcgtcgggg
      721 gcagcgcccg gcgcatcagg ctcagcgctt cgagcgggtc ggactccgca tccgcgctga
      781 tttccccctg aatcaggcat tcgatcatcg cggtgtgtcg agctctcggc atgctcagcg
      841 ttccggccgt ccccagtggc gagaataggc gaagcgggaa agtgaacctg agcactgttt
      901 cgcattctgt caaagacccc gatcaccgcg gacgccgcct cgcagggtgt ggcgtccgcg
      961 catcgggctt cggcgctatg ctgcccaccg cagacccgcc ctggcccgat cgagggcgac
     1021 cttgctgcgc tgagcagtac catcgaggcc gtttacgtcc cactcgcgaa gcgaagagcg
     1081 cgacaactac cggcagcgcg agccccacgc ggaggtggcg ccgcgggagc tcgcggggac
     1141 tgcctgccat catccggtag tgatttccgg ggcaggtccg gtcggtctcg ccgcagcagc
     1201 ctgcctggcg cgattcggaa tccgcaccgt ggttctcgag aagagcaccc gcgctggcga
     1261 gggcagccgc gcgatctgcc tcgcccgacg cagcctggag attctcgact ggatcggtgt
     1321 cgccgagcgc ttcgtttcca aaggactcgg ttggactcgc ggccatgcct tccatggtgc
     1381 gcatccgatc cacgagctgt gcatgcctca cagcgacgcc gagaagtttt ttcccatgac
     1441 caacctgcag cagtactaca tggaggagtt cctggcgcaa cactgcgctc gccaggaggc
     1501 gatcgagctg cggtggggca acgagctggt cggcattcag cccgagcccg acggggtgca
     1561 gctaagggtc gccaccaccg cgggcatgta tcagacgagc tgctcctggc tcctcgcagc
     1621 cgacggtgcc cgctctccgg cgcgaaggct tctgcagtta cccctctgcg gctcgtcctt
     1681 cgcgggccgc tatttgatcg cggacatccg cctgcggagc tcgtatccca ccgagcgccg
     1741 ggcctggttc gatccgccct ccaaccccgg ctcgacggtg ctgatgcatc gacagcccga
     1801 ggatatctgg cggatcgact atcagctgca ggagcacgac gatgacaggc gcgagagcga
     1861 gccgcacagg gtccgtgagc gcgtgcagcg gcacctcgac ttcatcggcg agcacggcga
     1921 atgggagctg ctgtggactt ccatctaccg cgcgcactcc ctggctctgg aaagctacgt
     1981 gcatgggcat gtgctgttcg caggcgatgc cgctcatctg gtaccgatct tcggggtgcg
     2041 cggcctgaac tccggtctcg ccgatgcgca gaatgtggcg tggaagctcg cctgggtctt
     2101 gcgcggcgat gcgcctgaga ggcttctgca cagctactcg agcgagcgcc gcaacgcgac
     2161 cctcgatatt caccgccagg cggagaagtc cactcgcttc atgaccccca gctctttggg
     2221 agagcgcctc atgcgtgcgg ccgtgttgtc cctcgcaagc cgacatgaat tcgtgcggcc
     2281 gttgatcgat ccacgccagt ccgtgcctta cacctatgcc ggcagtgagc tcaacacccc
     2341 ggaggaggca gccgccgggc tcgaggcttt tcggagtggc ccggcgccgg gagcgcccct
     2401 gcataacttg cgcatccgcg gtcactcggg cccgggatat ctgctcgagc ggctcgggac
     2461 gggattcacc gtgctgctgt tcctgccggg caagtggaca ggcgcgcacg ctgaatgtct
     2521 cgagcagctt cgcgccgtga ctcggctcga ctctcggggc gagttgcgcc ttctggtgct
     2581 caccggcgca ccgcctgccg agcgcgacag cagcgaatac gtggagcttc tgcaggatgt
     2641 ggcgggggtc gcgccgcaaa tctacgatgc gcgtccggga accgtttacc ttgcccgacc
     2701 cgacgggcac gtttgcgcga ggtggcgcgc ggccacggtg cgggatgtgg gtgttggttt
     2761 gtgtcgggct tgcacagtca tctgaggaca ggccatgcat tcacaaagcg gcgaaggccc
     2821 agacagctct ctggagcgtg cccacgcgct gctggtggcc gccatcgatt cgcagccacc
     2881 gggtacggag gcgctctacc tcgcgcgcct cgcgctgctg ctgctcaacg agctcgactg
     2941 ctccgaacgg gcgctagggc tcatcgaggc ggcccgacag ccgccttcta cggacccctg
     3001 agcgctcgcg gccccgctct aagaaccctt ggcgctcagc gcgccgcgcc accctcaggt
     3061 cgcgattgcc cgacccgcct gcagcacctc gctgacgatt tcggcgacat cggccgcgcg
     3121 ggtgcgatgg ttcacgaagc atgcccgcag ggccactttg ccgaacaccg tggcattgga
     3181 aagatagacg cggccgttgt cgatgacgcg cttgagaatc gcgccgttga gccggtcgag
     3241 gtcctgcgcg ccgccatcgc cacggtaacg gaaacacacc gcgctcaggt ccgtcggcgc
     3301 cagaagctcg agctccggac actcaccgat gcgcagactc aactgcgcgg catgatcgag
     3361 atcggcccgg atggcctcgc gaaatgcctg caccccgtga taacgcaggg acaaccagac
     3421 cttcagggcg cggaagcggc gcgagagctc gagggattcc tcgaagatgg caaggccctc
     3481 gatcgactcg ccttcgagca cgcgcgcgta atcacccgtg taggcaaaag cggcccgtgc
     3541 gcgcgccggg tctctgaaca gcaacaggcc gcaatccaca ggctggtaga gccacttgtg
     3601 cagatcgagc gaaagggaat ccgcgcgctc gaggcccgcg aacttctccg gacaggcaag
     3661 cgcggccgct gcgccgtagg cgccatccac atgcatccac aacccgtgtc tttcgcacac
     3721 cgcggcaatc gcctgcaagg ggtcaatcgc tccggtgctc accgtgccgg cggaggcgac
     3781 taccgcgacg agctctctgc ctgtggcccg atccgcctcg atcagatgct cgagcgcgcc
     3841 aacctgcatg cgcaggcgct catcaacggg gacgatgcgc atgttgtcac ggcccagtcc
     3901 caacagtccg acggccttgg ccatcgacat gtgtgcctcg ctggacacat acaccgtccc
     3961 cggccgcgcg ccccgctcat tcgccggcag gcgggcctcg cgcgccatcg cgagagccat
     4021 gaggtttgcc gttgatcccc cgccgcacag actgcccgtg agatgcgggc aaccgatcgc
     4081 ctcggcgatc cagccgacga cgatgcgctc gatggccact ccggcgggcg cagagcgcca
     4141 ggccgtgagg ttctggttga tgacactcgc caggagatca cccagcgcgg ccacgggctc
     4201 ccccgagccg agcacatatc cgaagaagcg cggccccgga ggccgtgaca gctgcacgac
     4261 acgctcgagt gcctcgagag cggccgcacc catgcctgca gccggcaggg gtccaccaaa
     4321 tgcttgcagc gtctgcattc cggtggtttg tggataggcc ggcaaatcct cgagccggcc
     4381 atagaactgc gcggccagct ccacgacccg ggtggcgagc tcgcggaact cctggctcga
     4441 aaccgacagc acaggagtcg atggcagtga ggaattcatg gccgcgagac tcctatttca
     4501 ccaggccgcg ctcgtagtag atgcggcagt tgagctcggc tgacagagcc gcggcgaccc
     4561 cgtgcagcgt cgcaatcagc gagcgcggcg gcggattgcc gatctcaggt gtcgtgccca
     4621 cgatgccgat gatgccgatg agctcatcct cagcgcgaaa caacggcgcc gagacggcat
     4681 tgatgccgag tctcacttca tcgaccgcct gttcgaagaa tctctcgcga atgccctcca
     4741 ggcgctgctc gatgagtgcg cgatcggtga tggagtggcg cgtgaaggcg gcgagctttc
     4801 cgccgagtac ccgctcgcgg gccggcgggt cggcgaatgc cagcgcgatg cgcccttggg
     4861 aggagcagtg gggtggcggc cgattcccgg gccgcgaaga gatcgacagc cgattgcgat
     4921 actcgacgca ggacagaacg atgggctcac cgttgcctgg aacggacagc agcgccgtct
     4981 ggtgggtctc atcgcgcagc ctggtgagat agggagcggc gagaaagcgc agatcgaatt
     5041 gctcgagcgc cgcctcgccg agggtgaaga gcttgccgcc caggctgtag ccgccgttgc
     5101 ggggatcctg gaagaccact cccaggcgct tcatggtcga cagatggcgg tacacgcgcg
     5161 gcttgagctc tccgagccgc agagcgagat cggtcaggcc gacggggcca tgcgcatccg
     5221 agagctcatc cagaatgcgg aacgcggttt gcaacgactg caacccgtcg ccgctctcgg
     5281 gctgggcgcc ccgctctccg ctgcgatgac ctggcttgtg cttcatgagg gctcgccgcc
     5341 tgcgccttcg tccgagggat tcgcgagcgg cccgcctgcg cgctataata cccgaaactg
     5401 cgtttcttca ggcgaaacaa ttcggcccat caccgccggg agccgccatg tttctcaaga
     5461 actgctggta cgttgccgca ctcgatcacg agctgatcga tggcaagctg ctgagccgca
     5521 tgatcctcgg cgagcatctg ctgctctatc gcggcgagag cggcaaagtc attgcgctca
     5581 atgaccgctg tccccaccgc ggcgcactcc tctcgcgcgg tcgcctcgaa ggcgacgcgg
     5641 tccgctgcat gtatcacggg atcaagtacg acggctccgg caaatgcgtg cagattcccg
     5701 gccaggacat gattccgccc aagctgcggg tgcgtgccta tccggtcgag cagcgcagcc
     5761 acttcgtctg ggtgtggatg ggcgatccgg ccaaggccga cccgtccctc atcgtggacc
     5821 tgccatacct tcacgagccg cgctggaagg gcttgcccgc ctacctgcac tacgatgcga
     5881 attacctgct gatcgtggac aacctcagcg acttcgccca cctcgcattc gtgcatacca
     5941 agacgctcgg cggctcggag gagtacgcct acgtcagcaa gcccacctcg attgagaggc
     6001 tcgaacgcgg cttccgtgtc gagcgctggc acctcaacag cgacccgccg ccgttccaca
     6061 agaaagtcat tccggcggct gagaagacca gcagggtcga ccgccgtaac atcgccacca
     6121 tgctgatccc cggcatcttc accatggaga cgctgtttgc gccggcgggc agcggcgcgc
     6181 cacagggcaa catcggcgat gccaaggaat accgcaactg ccagttcatg acgcccgaga
     6241 ccgagcgcag cacgcacttc ttctgggcgt acctcaacaa ctacgagggc caggactcca
     6301 ccatctccat ctcgctcctc gacagcctga tcgagggatt catggaggac aaggccatca
     6361 tcgagcggca acagcagacg ctcgacgagg acccggcctt cgagaagcag atgcttgcga
     6421 ttctcgccga tgcgccgctg gcgcatttcc gccgggtcct cggtaaactg ctggaagcgg
     6481 agcactcggc ggcctcgggg ccgccgagtg cggcaaccac gccggaaggc gcgcggctca
     6541 caacggcagc cggctaggcc caaccgcgat ccaggtcgag ggacgacaac aacagcgatt
     6601 tcgcgcgtat tgcatttctg taggggggag catgctaacg atcaagcgat tcctggccgg
     6661 agcttgtgtc ctggcggtgg ccgcgcctgc tgtgcaggcc gcgacaccgg cggacgcgag
     6721 caccagcagc actgggggcg aggcgctcga agaagtcatc gtgacggcgc agaagcgcgc
     6781 ggagagcgag caaaacgtgc cgatgtccat cacgaccttc ggtagcgtcc agctgcagga
     6841 aaaagcgatc aacaatttct tcgactacgc caccaaggtt cccaacctgg cgttcgctcc
     6901 gaccggcgat ggcgtgggca ccgcccggac cgtttcgatc cgcggcatct ccggtgacaa
     6961 cgtgactggc ttctatatcg atgacactcc gttgccggac tcgatcgacc cgcgggtgct
     7021 cgacatcgat cacatcgagg tgctgcgcgg tccgcaggga accctttatg gcgcgcgctc
     7081 catgggcggc actgtgcgca tcatcaccaa ggtgcccgat ctgacgggct tctcggctac
     7141 ggtgcacgcg gccgcctccg ataccgatca cacggatgag cccaactaca ctttcgatgg
     7201 catcgccaat attccgatcc tgagcgaccg cgtcgccttg cgactcaccg gcttctatga
     7261 caaccaggcg ggctggttca agcgcagctt ctgcaccgat ccctcgacgg ccgggacgag
     7321 ctgcttccct ctgtcaacga ccggacgcac gacgctcgac aatatcgcgc agaatgacac
     7381 cttcggtggc tcggcaagtc tcaccatcaa gctcagcgat gcattgacca tcacgccgcg
     7441 cgtcatgatg cagcgcgcgc gctacaacgg cttcccgatg gcggacttca acagcatgcc
     7501 caataacggc atcggattcc ccgccccgtc gggtccctac acgctgccgc agccgctggt
     7561 ctccaacgat ctcacgcagg cgaggttctt cgacgtgccc gagggtggca cggacaagtg
     7621 ggatctgtac tcggtcgcag ttcactggaa gaccgacctg ggtgagctcg tctcctccac
     7681 ggcctacttc gaccgcaaag tggacgagac cgaggatcaa tcggaattca tctgggccgc
     7741 gatcacctcg ggcgcgtgcg caacacccgc gccctactgc aatccgccgg ctccggggcc
     7801 gatcgaggag atcaaggact atcagcggtt cgtcgaggaa gtgcgctttg cttccagcct
     7861 cgccggaccg gtgcagttcg tggccggggc gttctattcg gacttccacg gcgccatccc
     7921 gttcgcgagc ctctacccac cgtcgcaggt cccgaatctc gataacgagc tgacgggcgg
     7981 attccccaac aatcccgacg gcatcccgaa cctggtgttt gcgcagaatt accatacgga
     8041 catcgaggag ccggcagtgt tcggcgaggt gtcctggaat ctcaccagcg cactgaagct
     8101 gacgggaggc ctgcgctggt atcaggtgaa gaccacatca gccggtaccg aggagggtct
     8161 ggccacgggt agcgatacgc tggtcgtcgg gccgcgcgct accgataagg aaaacggcac
     8221 gaatcccaag gtacagctgg actaccacct cacacccgat gagatggtgt atgcgctcgc
     8281 gtccaagggt ttcagacccg gtggagtggt gccgatcgtg ccgccgggca cccccggtac
     8341 ggccaatgac tgtgtcgccg cgctcgcggc ggtgaatccc aagctgaatc tcgccgatac
     8401 ccgcagcttc aagtccgact cgttgtggaa ctacgaggtc ggcaccaaga cggcctggct
     8461 cgatcaccgg gtcattttca atgcttcggc gtttgacatc cgctggaaaa acattcagca
     8521 gccgattctg ctcggctgcg gcttccagtt cacctccaac gcgggcgccg cggaaagcaa
     8581 agggggcgag ctggagttgc gcggccgcgc cacggagcaa ctcgagggct cgctcggtgt
     8641 cggttaccag gatgcgaaga tcacggagaa aggcaattca ccgcagccgg caggctcacc
     8701 ggtgtttcag gtccccaact ggaccggcaa tgcgtccgtc acctacacgg tgccgctcgc
     8761 gaacggctgg aatgcggcga gcggcctgga ctattcctat atcggcagca gcttcagttc
     8821 gagcaacggc cccgtcctgc cgcgtgagcg gccggcctac cgcctgctcg atgcccgcct
     8881 cgcaatgacc aacggttcga ccgaggtcgc tctggtgggc aagaatctga ccaacgagct
     8941 cacggacctg ggtgacaacc gctcgattgc cgccgaagtg cccggccggc cgcgcctgtt
     9001 cgtcaaccag ccgcgcacga tcggcatcga ggtgcgccag tccttctgag tcagccgccg
     9061 ctggccgcgc tcgcggccgg cgcagcttcg atcgagtccg cggccggctc ggcttcgact
     9121 gcggtcgggg ccggcccggc ggcggccgct gcgcccgccg ggggcagcgt gaagtaaaag
     9181 gtggttccct tcccgaccgc gctctcggcc cagatgcgcc cgccgtggcg ctgaatgatc
     9241 cgctgcacga tggacaggcc cacgccggtg ccttcgaatt ccgactcgga gtgcagccgc
     9301 tgaaagacgc cgaacagttt gcccgcatac tccatcttga agccgacgcc gttgtcacgt
     9361 acgtgataaa cgagctcctg tccgcgctgc aggcagtcga tctcgatgat cgccggatcg
     9421 cgctgacggg tgaacttgaa agcattcgag aggaggttca ccagaacctg ctccagcagc
     9481 gagccgtcgg catgacagct gccgagattg ccgacccgca cctcgacacg ccttgtggca
     9541 tcgcgcgcca gcagatcggc cgtgagtcgg cgaacgagcg gctcgagggc cacggtgccg
     9601 cgcaacaccg gccgtctgcc cagttgtgac agcgccagca gatcctcgat cagctcgccc
     9661 atgcgccggg cgccggcctc cacacgctcg agcagtggcc gaccttccgc gggaatgctc
     9721 gcaccgaaat cctccatgaa catctggcag aacccctgca cggtgcgcag cggagcccgc
     9781 agatcgtggg aaaccgagta cgagaacgac tccagctcac gattgacgac ttcgagctgg
     9841 cgattcgcag attcgagctc ctcggtacgc tggcgcactc gctcttcgag ctcacggttc
     9901 gccaccctga gctgacgcat ggagattgcc ctgttgagca caggcagcat cgcattcaac
     9961 ttgaagggct tgacgatgta gtcgagggca cctgcctgca tcgccttgac ggccgtatcg
    10021 atcgcggcat gcccggtcat cacgatcgcc atgacgtcgg gcgagatggc gcgggcagag
    10081 gtcagcagcg tgatgccatc catctcgggc atcatcaggt ccgtgagcaa caggtcgaac
    10141 tcctcggacc gcaaggtttc cagcgcgcgc ttcgccgagg tgaaccccgt cgtcgaatag
    10201 ccctcgacag acagcgtctc gcacaatgct ttcatctgcg ccgactcgtc gtcgacgatc
    10261 aacaggcggg caatcggggc tttcatggtt cgccgtgcgc ctcctcattg agcttcggca
    10321 atcagctcgg ccagtgcgcc gcgcagctcc tggagccggg gcggtttgtt cagaacgcgg
    10381 ttcacgtgcg ctggaacgtc attctcggcg accagccgat gaccccaccc ggtgagcatg
    10441 atgacagggg tccgcgcaga agctgccttg atcgcggcag cgaccttcct tccatccacg
    10501 tacggcatac cgaggtcggt gatcacgacg tcgaaccgcg gcccggccgc gacggccgca
    10561 ctgaaggcat cgatgcccgc ctgtccgccg ctggtggccg tgatgagatg accgtctcct
    10621 tcgagcgcat cgcgcaggga cttgatcagg agcgggtcat cgtcgaccag cagaatgcgc
    10681 agtcgctggg cgatcacctg cctcgggatg tgcacggtgg cggagatcgt ctcggtgaaa
    10741 gccgggaaga tcaagcggaa tgtcgtgccg cgtccgggct cgctttcgag ctcgagctcg
    10801 gcgctgtgcc gctgcaccat gccgtagacc atggccagac cgagaccggt gccgcgctcc
    10861 cccttcgtgg tgaaaaacgg ctccagacag cgacggcggg tttcctcatc catgccgatg
    10921 ccggtgtcgc tgacctccat gcagacccga tcctcggcat gttgctgctc gctctgcgcc
    10981 gaagacaaca ggcgtgtccg caacgtcagt gtcccgcctt ccggcattgc atcgaccgca
    11041 ttgaagatca ggttggtgag ggcatcgcgg atctcgctct cggcccccat cagctccgcc
    11101 ttgccgctca ccagctcggt gtgcagatcg acgacgacac cgcgttgttg cggcagatca
    11161 ctccagcgtg ctttggtgag atcgatcacg tggcacagga tccggttcgc atcgatgcgt
    11221 gccagctcga gctgcggctc gcgctggcgg tagaactcgc gcatgcgcgc taccgttgcc
    11281 gcgacgtcct cgatggcgcg ctggatcgtg accaggtagt tgcgtgcgcg ctcgcttaag
    11341 ttcggttccc gctcgagcag ggattcggtg tacagggaca ccggcgagat cgcattgttg
    11401 atgtcgtgcg cgataccgct cgccatctgc cccagtgcgc gcagccgttc ctgctgcatg
    11461 acggtgtgct gggtctggcg caggtcctcg taggcctgct gcagggccgt atagagccgc
    11521 atctggtgcg aagcgagggc gacgtgctcg gtcaggtgac gcagaaactc gcagtccgcg
    11581 ctcgcaaagc cattctcacg caagcgcgcg gcaatcagca cgcccgccac gcgattctcg
    11641 ataccgagcg gggcaatcac cagcgatcgt aaaccggcgc gcgcgaggcg ttgtgggaat
    11701 gggaaaggca cttcgggcac atcgggctcg taaacgagct cgccgcgaat gcagcggctc
    11761 aggccattac tgtcgagcgg gatggaggcc tgcgaaccga agcccagctg cgagccgagc
    11821 gccgagctgc cgaggctcgc caccttgagc tcgtctccgt ccacgagtcc gatacagccg
    11881 aaatcgatcg gcaggctctc ctccaggctg cgcagcacga tgcc
//
LOCUS       DASRHN010000172         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_386480, whole genome shotgun sequence.
ACCESSION   DASRHN010000172 DASRHN010000000
VERSION     DASRHN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386480"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..726
                     /gene="mobA"
                     /locus_tag="VFB37_06690"
     CDS             124..726
                     /gene="mobA"
                     /locus_tag="VFB37_06690"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HZO22177.1"
                     /translation="MSLDISDITGLVLAGGRGSRMGGVDKGLQEHRGVPLALHALRRL
                     APQVGRAMLNANRNLAVYESMGVPVWPDEIADYPGPLAGMLAGLAHCATPYLATVPCD
                     TPNFPLDLIAQLSGGLQESDSDIAMAFTQEDGKPRRQPVFSLMKVSVRESMVDFVRRG
                     EGNVGLWASQQRCARVLFEDAGAFYNANTLAELQRLQGES"
     gene            complement(751..3639)
                     /gene="fdhF"
                     /locus_tag="VFB37_06695"
     CDS             complement(751..3639)
                     /gene="fdhF"
                     /locus_tag="VFB37_06695"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZO22178.1"
                     /translation="MNAITRMERAQSAVPEITFQLDGRELTARSNETIIEVADREGIE
                     IPRLCYKPGMDAVGNCRACMVEIKGERVLAASCCRTVQAGMEVTSNNERVRKSQKMVL
                     ELLKSDMPEISLTRYNEVDDWAARLKVGKPRFAPRPSVPADLSHAAMAVNLDACIQCT
                     RCVRACRDEQVNDVIGLAFRGQHEKIVFDMDDPMGASTCVACGECVQACPTGALMPAR
                     EVALAVPDKSIESLCPYCGVGCQLTYHVKNDQISYVDGRDGPSNHSRLCVKGRYGFDY
                     SRHPQRLTVPLIRRADAPKRGDFVMDPNNVMQVFREATWDEALEVAGSALRRLRDTHG
                     PKSLAGFGSAKGSNEEAYLFQKLVRVGFGSNNVDHCTRLCHASSVAALLEGIGSGAVS
                     NPVMDVTNAEVVMLIGANPTVNHPVAATWLKNAVRSGTKLIVMDPRVSDLSRLAYRFL
                     QFKADTDVALLNAMMHVIIEEKLTDTKFIAERTEGYEALAENVKGYSPELMEPICGVP
                     ADTIREVARLYATSRGSMILWGMGISQHIHGTDNARCLIALAMMTGQIGKPSSGLHPL
                     RGQNNVQGASDAGLIPMVYPDYQKVEDPKVRQRFASAWGVPAEKLDPEPGLTVVEVIK
                     GALAGKVKGMYVMGENPAMSDPDANHAREALAKLEMLVVQDIFLTETAYLADVVLPAS
                     SFAEKFGTFTNTDRMLQLGRQALNPPGQARQDLQIIVQLARQLGLEWNYGTGEAAVAA
                     VFDEMRHTMPSIGGITWARLQAEHGVTYPCEKEGDPGTPVVFTDSFPTATGRGRFVPA
                     DIIPADERPDTDYPFVLITGRQLEHWHTGSMTRRSGVLDAIEPDPVASMHPKDIAALG
                     AKPGDLVTIASRRGKISLYARADDGTPHGAVFVAFCYYEAAINRLTNSALDPFAKIPE
                     FKYCAVRVTLGGAAPRQLSFGGGQILTEEDSGFTQAPATAAAEEEAPV"
BASE COUNT          623 a         1214 c         1182 g          672 t
ORIGIN      
        1 gcccacgctg cacgtgccct tgaaagcagc tctagcgccg atgtgcgtcc ccgtgtattc
       61 ggccagtcgg cgcaaggggt acgatggcgg tacttcgcgc agaagcgatg attattcgta
      121 gccatgagtc tcgacatcag cgacatcaca ggactggttc tagccggagg acgcggcagc
      181 cggatgggcg gggtggacaa gggcctccag gagcaccgcg gcgtgccgct tgcccttcat
      241 gcgctgcgcc gtctggcgcc gcaggtgggc cgtgcaatgc tcaacgcgaa ccgcaacctg
      301 gccgtgtacg aatcgatggg cgtaccggtc tggccggatg agatcgcaga ttaccccggc
      361 cccttggccg gaatgctggc cgggctggcc cattgcgcga cgccgtatct ggcgaccgtg
      421 ccgtgtgaca ccccgaattt cccgctggat ctcatcgcgc agctcagcgg gggcctgcag
      481 gagtcggata gcgacatcgc gatggccttc acccaggagg acggcaagcc gcgtcgtcag
      541 ccagtcttca gcctgatgaa ggtctcggtg cgcgagagca tggtggactt tgtgcgccgc
      601 ggtgaaggca acgtggggct gtgggccagc cagcaacgct gcgcccgggt cctgttcgag
      661 gacgccggtg cgttctacaa cgccaacacg ctagccgagc tgcagcggtt gcagggcgaa
      721 agctgagctc ggcggctcaa gccgccgagc tcagaccgga gcttcctctt cggctgctgc
      781 ggtcgcaggc gcttgcgtaa agcctgagtc ttcctccgtc aggatctgcc caccgccaaa
      841 gctcagctgc cgcggcgcag cgccgccgag cgtcacgcgt acggcgcagt acttgaactc
      901 gggaatcttg gcgaacgggt ccagtgcaga gttcgtgagc ctgttgatcg ccgcctcgta
      961 gtagcagaag gcgacgaaca ccgcgccatg gggcgtgccg tcgtctgcgc gagcatagag
     1021 gctgatcttc ccacgacgcg aagcgatcgt gaccaggtcc ccgggcttcg ccccgagggc
     1081 agcgatgtcc ttcggatgca tcgaggccac cggatccggc tcgatcgcat ccagcacgcc
     1141 cgagcgccga gtcatgctgc cggtgtgcca gtgttcgagc tgccgtccgg tgatcagcac
     1201 gaatggatag tcggtatcgg gcctctcgtc cgcagggatg atgtctgcgg gcacgaagcg
     1261 gccgcgtccg gtagccgtcg ggaacgagtc cgtgaacacc accggcgtgc ccggatcgcc
     1321 ttccttttcg cacgggtacg tcacgccgtg ctcggcctgc agccgcgccc aggtgatacc
     1381 gccgatgctt ggcatcgtgt ggcgcatctc atcgaatacg gccgccacgg ctgcctcgcc
     1441 ggtgccatag ttccactcga gcccgagctg gcgcgccagc tggacgatga tctgcagatc
     1501 ctggcgggcc tggcccgggg gattgagggc ctgccgcccg agctgcagca tgcgatcggt
     1561 gttggtgaac gtcccgaatt tctcggcaaa gctcgatgcc ggaagcacga catcggccag
     1621 gtaggcggtc tcagtgagaa agatatcctg cacgacgagc atctcgagct tggcgagcgc
     1681 ctcgcgggcg tgattggcat ccggatcgct catcgccggg ttctcaccca tgacgtacat
     1741 gcccttgacc ttgccggcga gggccccctt gatcacctcg acgacggtga gacccggctc
     1801 ggggtcgagc ttctcggccg gcacgcccca cgcgctcgcg aagcgctgcc gcaccttcgg
     1861 atcctccacc ttctggtagt ccggatagac catcggaatc aaaccggcgt cggatgcacc
     1921 ctgcacgttg ttctgtccgc gcagcggatg cagtcccgag ctgggcttgc cgatctgacc
     1981 ggtcatcatc gcgagcgcga tgagacaacg ggcgttatcg gtgccgtgga tgtgctggct
     2041 gatccccatt ccccacagga tcatggagcc gcggctggtc gcgtagaggc gcgccacttc
     2101 gcggatggtg tccgccggga cgccgcagat gggctccatc agctccgggc tgtagccctt
     2161 cacgttctct gcgagcgcct cataaccttc ggttcgctca gcgatgaact tcgtatcggt
     2221 cagcttctct tcaatgatca cgtgcatcat cgcattgagc agcgcgacgt cggtgtctgc
     2281 cttgaactgc aggaatcgat aggcgaggcg ggacagatcg ctgacccgcg ggtccatcac
     2341 gatcagcttg gtgccgctgc gcaccgcgtt cttgagccag gtcgcggcca ccgggtgatt
     2401 caccgtcgga ttggcgccga tcagcatcac cacctcggca ttcgtgacgt ccatgaccgg
     2461 attgctgacc gcacccgagc cgatgccctc gagcagggcg gcgacgctcg aggcatgaca
     2521 cagccgcgta caatgatcga cgttgttgct gccgaagccg acccgcacca gcttctgaaa
     2581 caggtaggct tcctcgttgc tgcctttcgc cgagccgaat ccagccagcg atttgggccc
     2641 gtgcgtatcg cgcaagcggc gcagtgcgct gccggcgacc tcgagcgctt cgtcccacgt
     2701 tgcctcgcga aacacctgca tcacattgtt cgggtccatc acgaagtcgc cgcgcttggg
     2761 agcatccgcg cgccgtatca ggggcacggt caaccgctgc gggtggcgtg agtagtcgaa
     2821 accgtagcgt cccttgacgc acaggcgcga gtggttggaa ggaccgtcgc gtccgtccac
     2881 gtagctgatc tgatcgttct tgacgtggta ggtcagctga cagccgacac cgcaataggg
     2941 gcacagcgat tcgatgctct tgtcgggaac tgccagcgcg acctcgcgcg ccggcatgag
     3001 agcaccggtc ggacaggcct ggacgcactc gccgcaggcg acgcaggtcg aggcacccat
     3061 gggatcgtcc atatcaaaca cgatcttctc gtgctggccc ctgaaggcca ggccgatcac
     3121 atcgttcacc tgctcatcgc gacaggcccg cacgcagcgg gtgcactgaa tgcaggcatc
     3181 cagatttacg gccatcgccg catggctgag atccgccggc accgagggcc gaggcgcgaa
     3241 gcgcggcttg ccgactttca ggcgcgcggc ccagtcgtcc acctcgttgt aacgcgtgag
     3301 ggagatctcc ggcatgtccg acttcagcag ctcgagcacc atcttctgcg atttgcgcac
     3361 ccgctcgttg ttgctcgtca cctccatgcc cgcctggacg gtgcggcagc aggaggccgc
     3421 cagaacacgc tcgcccttga tctccaccat gcaggcgcgg cagttgccca ccgcatccat
     3481 gccgggcttg tagcaaaggc gcggaatctc gatcccctcg cgatcggcaa cctcgatgat
     3541 cgtctcgttg ctgcgcgccg tgagctcgcg gccgtcgagc tggaaggtaa tctcaggcac
     3601 cgcgctctgc gctctctcca tgcgggtaat ggcgttcatc gacgctcctc aggccaactc
     3661 gttcgggaag tacttcagca cacagtcgag c
//
LOCUS       DASRHN010000173         9712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1211972, whole genome shotgun sequence.
ACCESSION   DASRHN010000173 DASRHN010000000
VERSION     DASRHN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211972"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFB37_06700"
     CDS             <1..491
                     /locus_tag="VFB37_06700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZO22179.1"
                     /translation="QFTREAERAARSGRALSLILCDIDHFKVINDTLGHASGDEVLRH
                     FGTRLQRLVSGECSWIARVGGEEFAIVLPGVALPEATETARALCLGIAAEPFRIGGTS
                     LEVTASFGLCGVDRVFPQETDFAEHAFKAADEALYRSKRSGRNRVTTTTLPVPDLLCA
                     CG"
     gene            complement(525..1388)
                     /locus_tag="VFB37_06705"
     CDS             complement(525..1388)
                     /locus_tag="VFB37_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZO22180.1"
                     /translation="MRMDDQEESQNVEDVRGSSGFRPIHGIGLGTIVIALIGGWVFGI
                     NPLQILGLLSGAGGLQSGPAAPAQAPPANDAQAKFVSQVLRSTETVWTAAFQERGLTY
                     QDPHLRLFRDSYPTACGQGEAAAGPFYCPNDRIVYLDLSFFDLMTRQLGAPGQFAHAY
                     VIGHEVGHHVQNLLGILGSVDKQRLRADDAQSNALSVRLELQADCFAGVWATRSQREH
                     GWRLEPGDIESALNAASQIGDDTLQRRARGTVVPETFTHGTSAQRVAWFRRGVDGGRL
                     EACDTFSAAQL"
     gene            complement(1510..1872)
                     /locus_tag="VFB37_06710"
     CDS             complement(1510..1872)
                     /locus_tag="VFB37_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZO22181.1"
                     /translation="MGMSAAASDAIRAKKVLGTNVQDPTGNKIGTIEDIVLDKTSNNI
                     VFAIVGFGGFLGMGEKYHPIPWSSLKYNEREGGYVVNYSKEQLKAAPADSIDALVANN
                     ATAYRDRSFDYYKAPHYW"
     gene            complement(2127..3104)
                     /locus_tag="VFB37_06715"
     CDS             complement(2127..3104)
                     /locus_tag="VFB37_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZO22182.1"
                     /translation="MRHEPSSRQSVTAALIGNLLVAATKIMAAVWTGSSAMTSEAVHS
                     LVDSGNEILLLYGQHRAAKRPDAAHPLGHGRELYFWSFIVALLVFAVGSGVSLYQGIV
                     QIRELHPIQDVRVSYIVLALAFIFEGASWLVAVRQFRTMRRRGADSLFESFRRSKDPP
                     AFMVLFEDSAALLGVLIAALGTYMAASLQMPVFDGIASILIGLVLGAIAILLARESKS
                     LLIGETASHELSESIRSIAARDPAVSRVNGLLSAQLAPDQILVALSLEFADELRVPQI
                     EEHVLQIEQNIRARHREVVALFIKPQTPHAFTEARKRRFGGEFPADSAL"
     gene            complement(3104..3682)
                     /locus_tag="VFB37_06720"
     CDS             complement(3104..3682)
                     /locus_tag="VFB37_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HZO22183.1"
                     /translation="MSPMRLALAIALAALGAGCASSPPTQFFTLEPIPASHPAPEVPH
                     GPIQIVAVHIPPLLDRDQIVREGSPGALIVSDRHRWGAPLGEMMRGVLTQDLLLRLPA
                     GAVVLPQQPAPPQTNTLTVDVLRFVADASGNVILEGAWSLVPPGSDTSTVNLGIHITE
                     PAGGADYAQQAHAMSLAVAQLADEIAGLVSRR"
     gene            complement(3679..5004)
                     /locus_tag="VFB37_06725"
     CDS             complement(3679..5004)
                     /locus_tag="VFB37_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO22184.1"
                     /translation="MTEPPSESPPPARTVAMARRSHWPGWIWGIPIAAVGVVVWLMLR
                     ATTRRGEEVTVIFDDASGLSTESTKVLYRGVDVGQVTDIELSSDRRHVTVTLDIDKRM
                     KPDLNRNTGFYLEGAEPSLSDLSSLKAVVAGPMIVMLPGSGPPTSHFTGIEGKPPKPL
                     DAHVSYSVTFSGDVGDLEPGSPVTLRGFPVGEVAQTELRVDAQTGAITTPVVLWLDPT
                     RFHMSAAKPADANWAPLMNATLARMVQHNLRARLSQDPPLIGARQVELASVPDVPEAR
                     LAMQGRYPEIPAVEDAGMANLPHELGQLPIRQIGDNVRAITERVKNLVSSPKLDESIG
                     HLEQTLAELDSTLKAVQPQVAPTLDSIHRTVDTLRHAAGEIDATSAAARKIMGANAVA
                     PDGNLQQALREMAGAARAIRTLASYLDEHPEALIRGRAGEVTLRDQVVR"
     gene            complement(5001..6209)
                     /locus_tag="VFB37_06730"
     CDS             complement(5001..6209)
                     /locus_tag="VFB37_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HZO22185.1"
                     /translation="MTIACSHCGTLQELPPLPRGAEAACPVCDHRLERTSGRSLDAAL
                     TCSLATWLLLFPANLLPLMSVSMLGATRETRAGSGVVTLWQGQRVIVAVLVAAFVLVL
                     PILRFGFLSAVLASLRLGRRPAWLGRAFRYAMALDFWAMPDVFLIGCAVGYSRVAANL
                     TVKLGSGGICLVFAAVFAMLSRATLDRRTTWRSISQDPQAVAPDEPSISCTVCDLAYP
                     EHAEGSACTRCGARLKARKTDSQVRTAALLIAGFVLYIPANYFPMSTDVQLGTTVPHR
                     IIDGIEDLVTAGLWPLGVLIFCTSIAIPLLKLLGLGWLWLSVRLRSKRHRLFRTRLYR
                     AIDEIGRWSNIDIFTIAVFVPLLQFGTLATARAATGATAFLLVVVLTMIASQVFDPRL
                     IWDEPQPERA"
     gene            complement(6384..9524)
                     /locus_tag="VFB37_06735"
     CDS             complement(6384..9524)
                     /locus_tag="VFB37_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22186.1"
                     /translation="MKLTRGVLEILTSLILLGLSPSAALTAPASGPQSLPLHWRSIGP
                     FRGGRVLAVTGVPGQPNHFYFGSVNGGVWETRDAGRTWSPIFDSAPVGSIGALAVAPS
                     DPRILYAGTGEADMRSDIAQGAGMYRSADSGQTWQAIGLEDSQQIGRILVDPSNPDVI
                     LVAALGHPYGANEMRGVYRSLDAGKTWSRTLYRDANTGAIDLAAEPGSLKIVYAALWQ
                     TRRPPWSVYPPSSGPGSGLFKSLDGGQTWTALAGQGLPANPGRIGLAIARSRAQTVYA
                     LIDASEGGLYRSDDAGASWVRVSADSRIWNRGWYFGGVTVDPSNAEIVYICNTAMYRS
                     TDGGKTFVPVKGAPGGDDYHSLWIDPNDAQRRILGVDQGALVTLNAGTTWSSWFNQPT
                     GQFYHVVTDNRFPYWVYGAQQDSGAAGIPSRTDNIDGVNLRQFHELVAGGESDNVAPD
                     PDDPQIVFGGRVDKLDRRTGQTRSVPPTLAYPEVYRATWTLPLVFGKAGSHALYFGNQ
                     RIFRTLDRGTHWEAISPDLSRETPEVPQNLDPPAIADTDQAGARRGVVYSIGPSPLDE
                     KLIWAGTDDGLVWRTRDGGKHWDNVTPPALTAWSKIASVEPSPFDAQSAYIAVDRHRL
                     DDSRPYIYRTHDGGTSWTLVVDGIADLSILNSVNVVREDPVRHGLLYCGTERGVYFSL
                     NDGTSWKPLQQNLPRTSVRDLQIHTDDLVIATHGRGFWIMDDVAPLRSLAADPSGTTR
                     LLAPAKAYRVQPTGFTGTPMPQDEPMGANPPGGAYIDYALETDSAPVKLSILDQQGAV
                     VRLFSSDDHAQGPDLAKIEIAPEWILPPEPLATAAGLHRFVWDLHYPPKAALHGEESS
                     EPDLGLWAPPGHYAVVLETGGHKYRQTLTVAPDPRLHLPAQAYAQQFSLARRIEGARL
                     QIAAALGEADRLHTAIGAEEAKASDALKSALHAADQQLTAVIDLAPPKRSPDSLGEAP
                     RSIDSLRYLETAFRQLAQSVEGADAMPSPDAVHGYAAHRVVLDRILHQWAGFKADTLR
                     HLNSQLQAAGAAPIAP"
BASE COUNT         1710 a         3122 c         3266 g         1614 t
ORIGIN      
        1 ggcagttcac tcgcgaggcc gagcgcgccg cgcgctccgg ccgtgcactg tcgctgatcc
       61 tgtgcgacat cgaccacttc aaggtcatca acgacacgct cgggcacgca agcggcgacg
      121 aggtactgcg ccatttcggc acgcggttgc agcggctcgt gagcggggaa tgcagctgga
      181 tcgcgcgcgt cggcggcgag gagttcgcaa tcgtgctgcc aggggtcgcg cttcccgaag
      241 cgacggaaac ggcgcgggca ctatgcctcg gcatcgccgc cgagcctttt cgcatcggcg
      301 gcaccagcct cgaggtgacc gcaagtttcg gcctgtgcgg tgtggaccgg gtattcccgc
      361 aggagacgga tttcgccgag cacgccttca aggcggccga cgaagcgctc tatcgcagca
      421 agcgttcggg ccgcaaccgc gtcaccacga cgaccttgcc cgtgcccgat ctgctctgcg
      481 cctgcggctg atcggagccg ctgcagccgc agcggcgcag aaggctacaa ctgggccgcc
      541 gagaaggtgt cgcaagcctc gaggcgccca ccatcgaccc cgcgccggaa ccaggcgacg
      601 cgctgtgcgc tggtgccatg agtgaaggtt tccggaacga cggtgccgcg ggcgcggcgc
      661 tgcagggtgt catccccgat ctgggaagcc gcattcaacg cggactcgat gtctcccggc
      721 tcgaggcgcc agccgtgctc gcgctgcgag cgcgtggccc acacacctgc aaagcagtcc
      781 gcctgcagct ccaggcgcac gctgagtgca ttcgattgcg catcatctgc gcgcagcctc
      841 tgcttatcca cactgccgag gatgccgagc aggttctgca cgtgatgacc gacttcatgg
      901 ccgatgacat acgcgtgcgc gaactgtccc ggcgcaccga gctgtcgggt catgaggtcg
      961 aaaaagctga gatcgagata cacgatgcgg tcgttcggac agtagaaagg gccggctgcc
     1021 gcttcgcctt gaccgcaggc cgtcggataa ctgtcccgga agagccgcag gtgcggatcc
     1081 tgatacgtga ggccgcgctc ctggaaagcc gccgtccaga ccgtttcggt gctgcgcagg
     1141 acctgtgaga cgaatttggc ctgcgcatcg tttgccgggg gcgcctgcgc cggtgcggcc
     1201 ggtccgctct gcaggccgcc ggcgccgctc agcaggccga gaatctgcag cggattgatg
     1261 ccgaagaccc agccgccgat cagtgcgatg acgatcgtgc caagtccgat cccgtgaata
     1321 gggcgaaatc cgctcgagcc acgcacgtcc tcgacgttct ggctttcttc ctggtcatcc
     1381 atccgcattg gggctgctcc gcgggcgcgc gctcggaatc gtgatgttac ctgaagaact
     1441 cgcgaagcat ggcagcggta agcatgcgga gcggcgatgg gccccgcatc tcctttcacc
     1501 gagtctggct taccagtagt gaggggcctt gtagtagtcg aagcttcgat cgcgataagc
     1561 cgttgcgtta ttggcgacca gcgcatcgat cgagtcagcg ggagccgcct tgagttgctc
     1621 cttgctgtag ttgacgacgt agccgccctc gcgctcgttg tacttcaggg acgaccaggg
     1681 aatcgggtga tacttctctc ccatcccaag gaagccgcca aagccgacga tggcgaacac
     1741 gatgttattg gacgtcttgt ccagaacgat gtcctcgatc gtgccaatct tgttgccggt
     1801 cggatcctgc acgttggtcc cgagcacctt cttggcgcgg atcgcatcgg aagctgctgc
     1861 actcatgccc ataacgatac ctccaggttg agagatggac gcaaagccag taatgagcgc
     1921 gcggggccgg agttccaccg cgcgaggcgc gtttcctttc ggaaggccac gcactaaaaa
     1981 gggccgtttt ggatgcggcc ctttgaggat tcgagagcct cgtctttcaa cagggatcga
     2041 atcgacggtt taagcttatt ccgggggtac gcggcgcggc aattcccctc gggcctctct
     2101 cgttgtaaga cttgtccgac tgtgcctcac aaggcgctat ccgccggaaa ctccccgccg
     2161 aagcggcgct tgcgcgcctc cgtgaaagcg tgtggcgtct ggggcttgat gaacaatgcg
     2221 acgacctccc ggtgtcgggc acgaatattc tgctcgatct gcaggacgtg ctcctcgatc
     2281 tgcggcacgc gcagctcgtc cgcgaactcg agactgagcg ccaccaggat ctgatcgggc
     2341 gcaagctgcg cactgagcaa cccgttcaca cgcgacaccg ccggatcccg cgcggcgatg
     2401 ctccggatcg actcggagag ctcgtgactg gcggtctcgc cgatcagcag actcttgctc
     2461 tcgcgcgcca gcagaatagc gatggcgccc agtaccagcc cgatcaggat ggaggcgatc
     2521 ccatcgaata cgggcatctg caagctcgcg gccatgtacg tgcccagcgc ggcgatcagc
     2581 acgcccagca gtgccgcgct gtcctcgaac agcaccatga aggccggcgg atccttactg
     2641 cggcggaagg actcgaagag gctgtcggcg ccgcgtcggc gcatggtacg aaactgcctg
     2701 acggcgacca gccaggaggc cccctcgaaa atgaacgcca gcgcgagaac gatatagctc
     2761 acgcgcacgt cctggatcgg atgcaactcg cggatctgca cgatgccctg gtagagcgaa
     2821 acgcccgatc ccaccgcaaa gaccagcagc gcaacgatga agctccagaa gtagagctca
     2881 cgcccatgtc caagcggatg ggcggcatcc gggcgcttgg ccgcccggtg ctggccatag
     2941 agcagcagga tctcgttgcc actgtccacg agcgagtgca ccgcctcgct ggtcatggcg
     3001 gagctgccgg tccacaccgc ggccatgatc ttggtggctg ccacgagcag gtttccgatc
     3061 agtgcggcgg tgaccgattg cctggaggaa ggctcgtggc gcatcagcga cggctcacca
     3121 gcccagctat ctcatcggcg agctgggcca cggcgaggct catcgcatgg gcctgctgcg
     3181 catagtccgc gccaccggca ggctcggtga tgtgaatgcc gagattcacg gtcgaggtgt
     3241 ccgagcccgg cggcacgagc gaccaggctc cctcgagaat gacgttgccg gaggcgtccg
     3301 ccacgaagcg cagcacgtcc actgtgaggg tgttggtttg cggcggggcg ggttgctgcg
     3361 gcagcacgac tgcgcctgcg ggcaagcgca ggagcagatc ctgagtgagc accccgcgca
     3421 tcatctcccc gagcggggcg ccccagcgat gccggtcact cacgatcagc gcaccgggtg
     3481 agccttcgcg cacgatctga tcccggtcga ggagcggcgg gatgtgcacc gcgacgatct
     3541 ggatgggtcc atgggggact tccggtgcgg ggtgtgaggc cggaatcggc tcgagcgtga
     3601 agaactgagt cggagggctc gatgcacagc ctgcgccgag ggcagccagt gcgatcgcaa
     3661 gcgcaagacg catcggactc atcgcaccac ctggtcgcga agcgtcactt cgcccgcgcg
     3721 tcctcggatc agcgcctcgg ggtgctcatc gagatagctc gccagcgtgc ggatcgcgcg
     3781 cgctgccccg gccatctcgc gcagtgcctg ctgcaggttg ccatccggag cgacggcgtt
     3841 cgcccccatg atcttgcgcg ccgcggcgct cgtggcgtcg atttccccgg ccgcgtggcg
     3901 cagcgtgtcg acggtgcgat ggatactgtc gagcgtgggc gccacctgcg gttgcaccgc
     3961 cttcagcgtg ctgtccagct cggcgagggt ctgctccagg tggccgatgc tttcatccag
     4021 cttcggtgag gagaccaggt tcttgacccg ttcggtgatg gcgcggacgt tgtcaccgat
     4081 ctgtcggatc ggaagctgcc ccagctcgtg cggaaggttc gccatcccgg cgtcctcgac
     4141 ggctggaatc tccggatagc ggccctgcat ggccagtctg gcctcgggca catcgggtac
     4201 gctcgccagc tcgacctgcc gggcgccgat gagcggtgga tcctggctca gacgcgccct
     4261 caggttgtgt tgcaccatcc gcgcaagcgt cgcattcatc aacggcgccc agttcgcatc
     4321 cgccggcttc gcggcgctca tgtgaaaccg tgtcgggtcc aaccacagca cgaccggcgt
     4381 cgtgatcgcg cctgtttgcg catcgacgcg cagctcggtc tgagccacct cgcccaccgg
     4441 gaagccgcgc agcgtgaccg gggagcctgg ctcgagatcc cccacatcgc cggagaacgt
     4501 gacggagtat gaaacgtgcg cgtcgagtgg cttcgggggc ttgccctcga tgcccgtgaa
     4561 gtggctcgtg ggcgggccgc tgcccggcag catcacgatc atgggaccgg cgacgaccgc
     4621 cttgagcgaa gacagatcgg acaggctcgg ctcggcacct tccagataga agcccgtgtt
     4681 gcggttgaga tcgggcttca tgcgcttgtc gatatcgagc gtcacggtga cgtggcggcg
     4741 gtcgctcgaa agctcgatgt ccgtcacctg accgacatcc acgccccggt acagcacctt
     4801 ggtgctttcg gtgctcagtc cgctcgcatc atcgaagatg accgtgacct cctcgccgcg
     4861 ccgggtcgtg gcgcgcagca tcagccatac gacgacgccg accgccgcga tcggtatccc
     4921 ccagatccag cccggccagt ggctgcgccg tgccatcgcg accgttcgcg ccggcggcgg
     4981 ggactccgaa ggcggctctg tcatgcgcgc tccggttgcg gctcatccca gatgagccgc
     5041 ggatcgaaca cctgcgaggc gatcatcgtc agcacgacta ccaggaggaa ggcggtggcg
     5101 ccggtggcgg ctcgtgcagt ggcgagggtg ccgaactgca gcaaaggcac gaagaccgcg
     5161 atggtgaaga tgtcgatgtt tgaccagcgt ccgatctcat cgatcgcgcg atacaggcgg
     5221 gtccggaaca atcgatgccg cttcgagcgc agccggacgg ataaccagag ccaaccgagg
     5281 ccgaggagct tgagcagcgg aatggcgata ctggtacaga agatgagcac gcccagcggc
     5341 cacagacctg cggtgaccag atcctcgatg ccgtcgatga tgcggtgtgg cacggtggtc
     5401 ccgagctgca cgtccgtgct cataggaaag taattggcgg ggatgtacag cacgaacccg
     5461 gctatgagca gcgcggcagt tcgcacctgg gagtcggtct ttcgcgcctt caggcgggca
     5521 ccgcagcgtg tgcaggcgga gccttcagca tgttccgggt aggctaggtc gcagaccgtg
     5581 caggagatgg agggttcgtc gggcgctacg gcctgcgggt cctgcgagat ggagcgccag
     5641 gtcgtccgcc ggtccagggt ggcgcgcgag agcatggcga acacggcagc gaagacgaga
     5701 cagatgcccc ccgagccgag cttgacggtc aagttggctg ccacgcggct gtagcccacc
     5761 gcacaaccga tcaggaacac atccggcatc gcccagaaat cgagcgccat cgcgtaccga
     5821 aaggcgcgcc ccagccacgc cggccgccgg ccaaggcgca gcgaggcgag gaccgcgctc
     5881 agaaatccaa accgcaggat gggcaacacg agcacgaatg cggccacgag cactgctacg
     5941 atcacacgtt gaccctgcca gagcgtgacc acgcccgagc cggcgcgagt ctcgcgggtc
     6001 gctcctagca tcgatacgct catgagcggg agcaggttag cggggaacag cagcagccag
     6061 gtcgccagcg agcaggtcag cgcggcgtcc agactgcggc cgctggttcg ctccagccga
     6121 tggtcacaga ccgggcacgc agcctctgcg ccgcgcggca acggcggcag ctcctgcaac
     6181 gttccacaat gggagcatgc gatcgtcatc ggttctcagc agtagccgcc cggcgcgtgc
     6241 cgagacagac gctgcgaagt taacgatcag tcgagcgctg cgttccagtg aaacgtcgag
     6301 ctgcagacta cgtgtcgcgt gggtcgggcg cggggcggcc cttcaccggt gctacccgcc
     6361 ctcgtcatcc gccccggagg ccatcaaggg gcgatcgggg cggctcccgc agcttgcagc
     6421 tgcgaattca agtgccgcag cgtgtcggcc ttgaagccgg cccattgatg cagaatacgg
     6481 tcgagcacca cgcggtgcgc ggcgtacccg tggaccgcat cggggctcgg catcgcatcg
     6541 gcgccctcga cagactgggc gagctgccgg aatgccgtct ccaggtagcg aaggctgtcg
     6601 atgctgcgtg gcgcttcgcc cagggagtcc ggtgagcgtt tgggcggggc gagatcgatc
     6661 acggcggtga gctgctgatc ggccgcgtgc aacgcagact ttagcgcgtc actcgctttg
     6721 gcctcctcgg caccgattgc cgtgtgaagc cggtcggcct cacccagggc ggcggcaatc
     6781 tgcagccgtg ccccctcgat ccggcgtgcc agggagaatt gctgcgcgta cgcctgagca
     6841 ggcaggtgaa ggcgcgggtc tggggcaacc gtcagcgtct ggcggtactt gtgcccgccc
     6901 gtctcgagca cgacggcgta atgccctggt ggcgcccaca gaccgagatc gggttcggag
     6961 gactcctcgc catgcagggc ggctttgggg ggatagtgaa gatcccagac gaagcgatgc
     7021 aggcccgctg cggtcgcgag cggctcgggt gggaggatcc attcaggtgc gatctcgatc
     7081 ttggcgagat ccggaccctg tgcgtgatca tcgctgctga acaggcggac cacggcgccc
     7141 tgttggtcga gaatgctcag cttgacggga gcagagtcgg tttcgagggc atagtcgatg
     7201 taggccccgc ccggcggatt ggcgcccatg ggctcgtcct ggggcatcgg tgtccccgtg
     7261 aagcccgtgg gctgcacccg ataggccttc gccggcgcga gcaaccgggt agtgccgctg
     7321 gggtcggccg ccagcgaacg cagcggcgcc acgtcgtcca tgatccagaa tccgcggcca
     7381 tgggtcgcaa tcacgaggtc gtcggtgtgg atctgcaagt cccgcacgga agtgcgcggg
     7441 agattctgtt gcagcggctt ccagctcgtg ccgtcgttta gcgagaagta gacgccgcgc
     7501 tccgtgccgc aatacaacag gccgtggcgt acgggatcct cgcgcacgac gttcaccgaa
     7561 ttcaggatgc tgaggtcagc gattccgtcg accaccagcg tccagctcgt cccgccgtcg
     7621 tgcgtccgat agatgtacgg tcgggagtca tccaaccggt gccgatctac tgcgatgtag
     7681 gcgctttgcg catcgaaggg tgaaggctcg acgctcgcga ttttcgacca ggcggtgagt
     7741 gcaggcggcg ttacgttgtc ccagtgcttg ccgccgtcac gggttcgcca gacgagcccg
     7801 tcatccgtgc cagcccagat cagcttctca tcgagaggcg atggaccgat cgagtagacg
     7861 acaccgcggc gggcccctgc ctgatccgta tctgcgatgg ccggaggatc gagattttgc
     7921 ggcacttcgg gcgtctcgcg actgaggtcc gggctgatcg cctcccaatg cgtgccgcga
     7981 tcgagagtgc ggaagatgcg ctggttgccg aagtagagcg catggctgcc ggccttgccg
     8041 aacacgagcg gcaacgtcca ggtcgcacga tagacctccg ggtaggcgag ggtcggcggc
     8101 acactgcgcg tctgtccggt tcgcctgtcg agcttgtcca cgcggccccc gaagacgatc
     8161 tgcggatcat cgggatcggg tgcgacgttg tcgctctcgc cgcccgcaac gagctcgtgg
     8221 aactggcgca ggttcacgcc atcaatgttg tccgtgcggc ttgggatacc ggccgcgccc
     8281 gagtcctgct gagcgccgta gacccagtag gggaagcggt tgtcggtgac gacgtgatag
     8341 aactgaccgg tcggctggtt gaaccacgag ctccaggtcg tgccggcgtt gagcgtcacg
     8401 agagctccct gatcgacgcc caggatgcgc cgctgcgcat cgttcgggtc gatccacagt
     8461 gagtgatagt catcgccgcc gggcgcgccc ttgaccggca cgaacgtctt gcctccgtcg
     8521 gtggatcgat acatcgcggt attgcagatg tagacgatct cggcattgga gggatcgacc
     8581 gtcacgccac cgaagtacca gccgcggttc cagatgcggc tgtccgcact cacgcgaacc
     8641 cagcttgcgc ccgcatcatc cgagcgatag aggccccctt cggatgcatc gatgagtgca
     8701 tacaccgtct gcgccctgga gcgggcgatg gcgagtccga tgcggcccgg attggccggc
     8761 aggccctgtc cagcgagcgc ggtccaggtc tgcccaccat ccagagattt gaacaggccg
     8821 ctgccgggtc cgctcgaggg cgggtaaaca ctccagggcg gccgccgggt ctgccacagg
     8881 gcggcataca cgatcttgag cgatcccggc tccgcggcca gatcgatcgc gccggtgttg
     8941 gcatcgcggt acagggtgcg gctccaggtc ttgccggcat cgagcgagcg atacacgccg
     9001 cgcatctcat tagccccata ggggtgcccg agcgcggcga ccaggatcac atccggattg
     9061 gaaggatcga cgaggatgcg gccaatctgc tgggaatcct ccaggccgat cgcctgccag
     9121 gtctgtcccg aatcggcaga acgatacatg cccgcaccct gtgcgatgtc cgagcgcata
     9181 tccgcctcgc cggtacctgc gtagaggatc cggggatccg aaggtgcgac ggcgagggcg
     9241 cctatcgagc cgacgggggc ggagtcgaag atcggagacc aggtgcgtcc cgcatccctc
     9301 gtttcccaga ctccaccgtt gaccgagccg aaatagaaat gattcggctg acccgggaca
     9361 ccggtcaccg ccagaacgcg cccgccccga aagggcccga tggagcgcca gtgcaaaggc
     9421 agggactgcg gccctgatgc cggggcggtg agcgccgcag agggggagag gccaaggagg
     9481 atcagggaag tgagaatttc cagcacgcca cgagtcaact tcactgcaca cctcgacgaa
     9541 acaaaagcac tgtcccgctc ctatacgcgc cgggcgcaag caacggcagc gcactatacg
     9601 cgcttgctca agcgcccggg attgcaccgc tgcggcgcga taccaacagc gtcctctgca
     9661 cgaccaatga tcgcgggcag aggttggaca ctcccgtcgg atggcagccg ac
//
LOCUS       DASRHN010000174         5384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_726180, whole genome shotgun sequence.
ACCESSION   DASRHN010000174 DASRHN010000000
VERSION     DASRHN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726180"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFB37_06740"
     CDS             complement(<1..293)
                     /locus_tag="VFB37_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO22187.1"
                     /translation="MNGLMQHWPLTTTSILRHARREHGSREIVTRSVEGDIRRYGYAE
                     LHRRALQVAHALQAAGLMAGDRVATLAWNTDRHMEIWYGVMGLGAVCHTITPRL"
     gene            449..3664
                     /locus_tag="VFB37_06745"
     CDS             449..3664
                     /locus_tag="VFB37_06745"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22188.1"
                     /translation="MVRVPALAATAVFTCLGLGPLVSVAASQQSPPGIFGDLAWRMIG
                     PFRGGRTKAVAGVPAQPNVFYIGAVDGGVWKTDDAGRTWRPVFDEQPTQSVGAIAVAP
                     SDPNVIYVGSGEGLHRPDLSVGNGIYRSSDGGRTWTHLGLPDAQQICEISVDPHDAKR
                     LFVAVLGHPYGPSPDRGIYRSTDGGASWQRVLYKDEDTGGSAVEIDPARPDTVYAALW
                     QARLGPWEDKNDFNGTGGGLFKSTDGGSSWKRLTQGLPSDLSQVNITVSASTPGRVYA
                     AVGTNEPGEYQSSAGLGVFRSDDAGESWTRITSDPRPALRIGGGDLPVLRVDPSNADI
                     LYATGIVTMKSQDGGKTWTALRGAPGGDDYQNLWISPRDGRIIALVSDQGAVVTVNGG
                     ETWSSWFNQPTAQLYHVSVTPTFPYRVCGGQQESGSVCTASRGNDGEITDRDWHPVGA
                     IEYGYVAPDPLDPDIIYGAGRNEVSRFHVSTGQVQNVTPIPVRGPNARADRTEPILFS
                     PLDPHTLYYAANRLYRTQDAGQTWQAISPDLAREDGGTPPSVGNLHVAGAERQRGVIY
                     SLGLSPVSIDTLWAGTDDGLVYVTRDGGAHWQNVTPPALTPWSKVTQIEASHSDAATA
                     YISVSRMRIDDLHPYIYRTHDAGKTWQPITAGLAQDAPVNAVREDTVRKGLLFAATES
                     AVWMSADEGEHWESLQLNLPHTSMRDLTIHDGDLILATHGRSFWILDDISRLRQITST
                     QMRNAVLLHPDPAWLMRRSTWTDTPIPPDEPLASNPPAGAIVEYFLPAQAKKPLVLEI
                     VDAHGSLVRRYRSDDSPEPSAEERARELIPHYWLEPSRVLPAQSGMHRWVWDLHYPAP
                     LSPTHGYPISAVPGATPREPQGPRAMPGNYVVRLMYDGRKLEAPLTLKADPREHVSSA
                     DLEQQFQLATHLAELLTTSSRTLMSAQSEQAQLNALESSGAAAEAVKSYEGRLSELLS
                     GAKEADKGPSPAEPKPTLTEVQSRIAALYGELSRADAAPTAVQLSATTAAQESLSRLQ
                     QSWQQLQAQLPALNARLRSAKLAVIRAELPPPRDRNVADEE"
     gene            complement(3699..>5384)
                     /locus_tag="VFB37_06750"
     CDS             complement(3699..>5384)
                     /locus_tag="VFB37_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22189.1"
                     /translation="WTEPVFVSPHDSNVLYTAAQFVLKSEDQGRSWKRISGDLTRNDK
                     SKQQPSGGPITLDITSVEYYDTVFTLAESPLMQGMLWAGSDDGLIHVTRDDGKSWQNV
                     TPKDMPPWSTVSMIEPSPHDLKTAFAAIDRHRLDDLSPLIYKTHDGGASWQRIVTGIP
                     QGSFVRAVREDPKKAGLLYAATELGVYVSFDDGGHWQALQLNLPVAPVHDLVVKDDDL
                     VAATHGRSFWILDDLTPLRQIEMSAAVADVRLYRPEPAYRLHFPIDVNRRRPVGDNPP
                     DGAVIDYFLKDKARPDEEITLEILDAQGKVLRRYSNHRKDKFEQPAEWPDREMPAELL
                     PDAAGMNRFPWDLRVEDPVQIPGAFYSDEGPRGPVVSPGAYTVRLTTSHGTQSQPLQV
                     VPDPRLSGSLTAADEAAHWDLVSKTAADIEALHRAVNQIRACRTELQKLQTDTHASEA
                     RSLGKSVSKLENEMAPVEEQLIQVNMKASEDNLRYPNRLDEQYDTFIATIDGDDFRPT
                     QPQLEVFGQLHGRLQQQLSAWKALRDKDLSALNAKLQASGAAPVTVPEGAPGM"
BASE COUNT          916 a         1806 c         1712 g          950 t
ORIGIN      
        1 aggcgcgggg tgatggtgtg gcagacggca ccgagcccca tgaccccata ccagatctcc
       61 atgtgccgat ccgtgttcca ggcaagggtg gccacccgat cccctgccat gaggcccgca
      121 gcctgcaatg catgggcaac ctgcagagcg cggcgatgca gctcggcgta gccgtagcga
      181 cggatgtcgc cctccacgga ccgcgtcacg atctcgcggc tgccgtgctc gcgcctggca
      241 tgccggagga tgctggtcgt ggtcaaaggc cagtgttgca tcagcccatt catgaaccgc
      301 tcccccctcc acggctcgca acgcgctgcg tacgccccgt attccggctc agctgatcga
      361 tcgcgctcaa tgctacacct gcgtgagccc ccgcgtgccg cgcgccagat gttatgcttc
      421 cgacccgatt ttcgagcccg ggatccttat ggtgcgcgta cccgccttgg ccgcaacggc
      481 agtctttaca tgtctcggcc tggggcccct ggtgagcgta gccgcctcac agcaatctcc
      541 tccgggcatc ttcggcgatc tcgcctggcg catgatcggc ccgtttcgcg gcggtcgcac
      601 caaggccgtc gcgggtgtcc ccgcgcaacc caacgtgttc tatatcggcg ccgtcgatgg
      661 cggagtctgg aagacggacg atgcggggcg cacgtggcgg ccggtcttcg acgagcagcc
      721 tacgcagtcg gtcggagcca tcgccgtggc gccctcggac ccgaatgtca tctacgtcgg
      781 cagcggcgag ggattgcacc gccccgatct gtcggtgggc aacggcatct atcgctcgag
      841 cgatggggga cgcacctgga cccatctcgg attgcccgac gcgcagcaga tctgcgagat
      901 ctcggtggac ccgcacgatg cgaagcggct gtttgtagca gtgctgggtc acccatacgg
      961 accgagcccg gaccgcggaa tctatcgctc cacggatggc ggagcgagct ggcaacgcgt
     1021 gctctacaaa gacgaggaca cgggcggctc tgctgtggag atcgaccccg cgcggcccga
     1081 cacggtttat gccgcactct ggcaggcccg gctcggcccg tgggaggaca agaacgattt
     1141 caacggcacg ggcgggggtc tgttcaaatc caccgacggc ggcagcagct ggaaacggct
     1201 gacccagggc ctgccttccg acctgtcaca ggtcaacatc accgtctcgg cgagtacgcc
     1261 cggtcgggtc tatgccgctg tcggcaccaa cgagccgggt gagtatcagt cctctgccgg
     1321 tctcggggtc ttccgttccg atgacgcggg cgaaagctgg acccgaatca cttcggatcc
     1381 ccgacctgcg ctgcgcatcg gcgggggcga tctgcccgtg cttcgggtgg acccctccaa
     1441 tgccgatatc ctctacgcca ccggcatcgt cacgatgaag tcgcaggatg gcggtaagac
     1501 ctggactgcg ctgcgcggcg ccccaggcgg cgatgattat cagaatctct ggatcagccc
     1561 ccgcgacggt cgcatcattg cgctggtgag cgatcaggga gcggtcgtca cggtgaacgg
     1621 cggcgagacc tggagctcct ggttcaatca gccgaccgcg cagctctatc acgtgagcgt
     1681 cacacccacc ttcccttacc gggtctgtgg cggacagcag gagagcggct ccgtgtgcac
     1741 cgccagccgc ggcaacgacg gtgagatcac cgaccgcgac tggcatcccg tcggggcgat
     1801 cgaatacggc tacgtggcgc cggatccact ggatccggac atcatctatg gcgcaggccg
     1861 caacgaggtt tcccggtttc acgtctcgac cggacaggtg cagaacgtga ctcccatacc
     1921 cgtacgtggc cccaatgcgc gcgcggatcg gacggagccg attctgttct cgcctctcga
     1981 tcctcacacg ctgtattacg cggccaacag actgtacagg acccaggatg ccggccagac
     2041 gtggcaggcc atcagtcctg acctggcgcg agaggacggc ggcacgccgc ccagtgttgg
     2101 caacctccac gtggcggggg ccgagcgcca gcgcggcgtg atctactctc tgggactgtc
     2161 accagtaagc atcgacacgc tttgggcagg tacggacgac ggcctcgtgt atgtgaccag
     2221 ggatggaggc gcgcactggc agaatgtaac tccgcccgcg ctgaccccct ggagcaaggt
     2281 cacccagatc gaggcctcgc actctgatgc ggccactgcc tacatctccg tgagcaggat
     2341 gcgcatcgat gaccttcatc catatatcta tcggactcac gatgccggca agacgtggca
     2401 gccgatcacg gcaggcctgg cgcaggatgc ccccgtcaat gcggttcgcg aagacacggt
     2461 gcgcaagggc ctgttgtttg ccgcgaccga aagtgctgtg tggatgtccg ccgatgaggg
     2521 tgagcactgg gagtccttgc agctcaacct gccgcacacc tccatgcgcg atctgacgat
     2581 ccacgacggc gatctgatcc tcgctacgca tggccgctcc ttctggattc tggacgacat
     2641 ctccaggctg cggcaaatca cttcaacgca gatgcgcaat gcggtgctgc tgcatcccga
     2701 tcccgcctgg ctgatgaggc gcagcacctg gactgacacg cccatcccgc cggacgagcc
     2761 tctggcgagc aatccgcccg ccggtgcgat cgtcgagtac ttcctgccgg cgcaggccaa
     2821 aaagcccctc gtgctcgaga tcgtggatgc tcacggcagc ctcgtgcgcc gctatcgcag
     2881 cgacgattct ccggagccga gcgcggagga gcgggctcgc gagctgatcc cgcattactg
     2941 gctggaaccc tcacgcgtgc tcccggcgca aagcggcatg catcgctggg tatgggacct
     3001 gcactatccg gcgccgctct ccccaacgca cggctacccg atctctgcgg ttccaggggc
     3061 gacgccccga gagccgcagg gcccccgagc gatgccgggt aactatgtcg tccggctgat
     3121 gtatgacggc cgcaagctgg aagcgccgct cacgctgaaa gcggatccac gcgagcacgt
     3181 ctcgagcgcc gacctcgagc aacagtttca gcttgcgact caccttgcgg agctgttgac
     3241 cacgagctcg cgcacgctga tgagcgcgca gtccgaacag gcgcagctca acgcgctcga
     3301 atcctctggc gcggctgccg aagccgtgaa gagttatgag gggcggttgt cggagctgct
     3361 cagcggcgcg aaggaggctg acaaggggcc ctccccggcg gagcccaaac caacactcac
     3421 cgaagtacag agccggatag cggcgctcta tggcgagctg tcgcgcgcgg atgccgcacc
     3481 gactgccgtg cagctctccg cgacgaccgc cgcgcaggag agcctgtcga ggctgcagca
     3541 gagctggcag caactgcagg cgcagctccc cgccctcaat gcgcgcctgc ggtcagcgaa
     3601 gcttgcggtg atccgcgccg aacttccgcc gccgcgcgac cgcaacgttg cggacgaaga
     3661 ataactcgcg ctcactgctc cgggcccggc ttcgggcctc acatgcccgg ggccccctcc
     3721 ggaacggtga cgggtgcggc gccggatgcc tgcagtttgg cgttgagcgc actcaggtcc
     3781 ttgtccctca gcgccttcca cgcgctgagc tgttgctgca gccttccatg cagctgcccg
     3841 aagacttcga gctgcggctg cgtgggccgg aagtcatcgc catcgatggt cgcgatgaat
     3901 gtgtcgtact gctcatcgag acggttggga tagcgaagat tatcctccga ggccttcatg
     3961 ttgacctgaa tgagctgctc ctcgacggga gccatctcgt tctcgagctt gctcactgac
     4021 ttgccgagcg aacgcgcctc ggaggcgtgc gtgtccgtct gcagcttctg cagctcggtg
     4081 cgacaggctc tgatctggtt gacggcacgg tgcagtgcct cgatatctgc cgcggtcttg
     4141 ctcaccaggt cccagtgcgc cgcctcatcc gcggcagtga gggagccact caggcgcggg
     4201 tccggcacaa cctgcagcgg ctgcgactgc gttccgtgcg aggtcgtgag gcgcaccgtg
     4261 taggcgcccg gactgactac cggcccccgc ggtccctcgt ctgagtagaa cgcgcccgga
     4321 atctgcacgg gatcctccac ccgtaaatcc caggggaagc gattcatgcc cgcggcatcc
     4381 ggcagcagct ccgccggcat ctcccgatcg ggccattcgg cgggctgctc gaatttgtcc
     4441 ttgcggtgat tggagtagcg tcgcagcacc ttgccctgcg catcgaggat ctccagggtg
     4501 atctcctcat ccggccgcgc cttgtccttc aggaagtagt cgatcactgc cccgtccggg
     4561 gggttgtcac ccaccgggcg gcggcgattc acatcgatcg ggaaatgcag ccggtaggcc
     4621 ggctccgggc gatacaggcg cacatccgct acggctgccg acatctcgat ctgccgcagc
     4681 ggagtcagat cgtccaggat ccagaaggag cgcccgtgcg tcgccgcaac cagatcgtcg
     4741 tccttcacga cgagatcgtg caccggcgcg acgggcaaat tgagctgcag cgcctgccag
     4801 tgcccgccat cgtcaaagga gacgtacaca ccgagctctg tggccgcgta gaggagcccg
     4861 gctttcttcg ggtcctcacg caccgcccgc acgaacgagc cctgcggaat ccccgtgacg
     4921 attcgctgcc agctcgcccc gccgtcgtgc gtcttgtaga tgaggggcga gagatcatcc
     4981 agccggtgcc ggtcgatggc ggcgaacgcc gtcttcagat cgtgtggtga aggctcgatc
     5041 atgctcaccg tgctccatgg cggcatatcc ttcggagtca cgttctgcca gctcttgccg
     5101 tcatcgcgtg tcacgtgaat caacccatcg tccgagccgg cccagagcat tccctgcatc
     5161 agtggggatt ccgccagggt gaagaccgtg tcgtaatatt cgacactcgt gatgtcgagc
     5221 gtgatgggtc cgccggaagg ctgctgtttg ctcttgtcgt tgcgcgtgag atccccgctg
     5281 atacgcttcc agcttctgcc ctggtcctcg ctcttcagca cgaattgcgc tgccgtgtac
     5341 agaacgttgg agtcgtgcgg ggagacgaag accggctcgg tcca
//
LOCUS       DASRHN010000175         4992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_549503, whole genome shotgun sequence.
ACCESSION   DASRHN010000175 DASRHN010000000
VERSION     DASRHN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549503"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /gene="gatB"
                     /locus_tag="VFB37_06755"
     CDS             <1..231
                     /gene="gatB"
                     /locus_tag="VFB37_06755"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247018.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HZO22190.1"
                     /translation="TADEIIREKGLKQITDSAAIERAIEEVMARSPAQLAEYRAGKDK
                     LFGFFVGQVMKATGGKANPAQLNELLKKKLPG"
     gene            334..1272
                     /locus_tag="VFB37_06760"
     CDS             334..1272
                     /locus_tag="VFB37_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZO22191.1"
                     /translation="MQNPSSPASDQVRRFIVENRPVRGHWVRLDSAWRELCAHAEYPL
                     PVRELLGQAVAASVLLAATLKFRGTLTLQLQGDGAVSLLVAQCTHDFRLRAVARFEES
                     AVAAIASAPQRDARIFRQLVGRSGQVAVTIEADERSARYQGIVPLSGGSLADSLEAYF
                     ATSEQLPTRVILAADSKCGAGVLVQKLPGPSNADESELEATWNDAQRGIESLSAADLL
                     ECPVEELLERGFAAQDLRLFRGSPVQFQCRCSPGRVVGLLRALGADEVRDVLREQGAV
                     TVTCEFCHRPYKFDAVDVESLFATGPDSTEGSPSLH"
     gene            1450..2391
                     /locus_tag="VFB37_06765"
     CDS             1450..2391
                     /locus_tag="VFB37_06765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO22192.1"
                     /translation="MASGPTALVTWLRATGEVSRIRLLSLCSRRDFSVSDLARALGQS
                     EPRVSRHLRILCEAGLLERLRQGQWVHYRLATQAAAAGFIQGVLAQLDRSDAQLSRDL
                     ERAQAPHASRETARPRDGSAASESRLGQALQGFVDANMPSSQQGSALLVGVEHLEVLE
                     SAASMAADCVAIAHSRRAAQGARAFAERRGLNCRVLLASAARGLNDRDIGGRTFDGVI
                     LDHPAAVDADFADLLASARNLLSATGKLCLFERYESLQISRERVVEHPLARLRRLLAD
                     SGLTCERLSPLEADGEHVLAAVAVPAAHAGLTNLRRA"
     gene            2388..3296
                     /gene="metF"
                     /locus_tag="VFB37_06770"
     CDS             2388..3296
                     /gene="metF"
                     /locus_tag="VFB37_06770"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004127280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZO22193.1"
                     /translation="MNTSNPPQLAWHDPHPSISVSFEFFPPADADMEVTLWKSVERLA
                     PLTPRFISVTYGADGSTRERTHNLVTRVQRETTLVGAPHLTCIGATQAEILEIARRYW
                     DEGIRNVVALRGDPPQGQAGYRPHPDGFAYAADLVAGLKRVADFDVSVAAYPEVHPEA
                     PSAEFDLDNLKRKVDAGASRAITQFFYDSGVYLRFRDRCAAAGIQACIVPGILPITRF
                     PQVLKFAARCGASIPEWLKERFEGLDEDAETRRLIAASVAIEQVQALQREGVREFHFY
                     TLNRSELTYAICHALGVRTANQRAVA"
     gene            3293..>4992
                     /locus_tag="VFB37_06775"
     CDS             3293..>4992
                     /locus_tag="VFB37_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZO22194.1"
                     /translation="MSICPHEEHTSIPWRADARKDGAAARAAAVPFGMESVDETARRE
                     RISRLKGLLDERIIVLDGAMGTMIQQHKLDERAFRGERFRAYGRDLRGNNDILCLTHP
                     DIVSEIHRAYLEAGADLIETNTFNSTAISQADYGTEALVPELNYRAAQLARAAADQFA
                     HASGRAAFVAGALGPTSRMASLSPDVNDPGYRSVTFDQLVATYTEAARGLLLGGVDVL
                     LIETIFDTLNAKAAIYAVQGLLDELSLPVPIMISGTITDASGRLLSGQTPQGFWNSIR
                     HARPLAVGLNCALGASQLRQYVQELGRVADTYICAYPNAGLPNAFGEYDETPEETAAI
                     LKDYGASGLLNIVGGCCGTTPAHIRLMRDAMGSFPRREIPASVPKCRLSGLEPLDIDA
                     DSLFVNVGERTNVTGSAKFRKLIEAGDYAAALEVARQQVLNGAQMIDVNMDEGMLDSE
                     SAMTRFLHLVAAEPDIARVPVMIDSSKWSVIEAGLKCVQGKPVVNSISLKEGEEAFIE
                     HARKVRRYGAAMVVMAFDEQGQADSIERKVAICKRCYDILTTRADVLPEDIIFDPNIF
                     A"
BASE COUNT          878 a         1576 c         1625 g          913 t
ORIGIN      
        1 acagccgatg agatcattcg cgagaagggg ctgaagcaga tcaccgatag cgctgccatc
       61 gagcgtgcca tcgaggaggt gatggcccga agtcccgcgc agcttgccga gtaccgagcc
      121 ggcaaggaca agctgttcgg attcttcgtc ggccaggtga tgaaagcgac cgggggcaag
      181 gcgaacccgg cgcagctgaa cgaactgctg aagaagaaac tccctgggta gaggcctacg
      241 gccgagatct gaagcggggt gcacccctga tcagatcccg gagaggagac agtccggttg
      301 agttgtgccg tcccatcccc catggtgggt gcaatgcaaa atccatccag tcccgccagc
      361 gatcaggttc gtcgcttcat cgtcgaaaat cggccagtac gagggcactg ggtgcgtctc
      421 gattcggcct ggcgtgagct gtgcgcccac gccgagtatc cgttgcccgt gcgcgagttg
      481 ctgggccagg ccgtagcggc ctcggtactg ctcgcggcca ccctcaagtt ccgcggcacg
      541 ctgaccctgc aattgcaggg cgacggggcg gtcagtctgc tcgtggcaca atgcacgcat
      601 gatttcaggc tgcgtgcggt cgcccggttt gaggagtctg cggtcgcggc gatcgcctcg
      661 gctccccagc gcgatgcgcg catctttcgc caactcgtag ggagatcggg ccaagttgcc
      721 gtcacgatcg aggcagatga gcgcagcgcg cgctatcagg ggatcgttcc gctgagcggc
      781 ggctcgctgg ccgactcgct ggaagcctat ttcgcgacct ctgagcagct tcccactcga
      841 gtcatcctgg ccgccgacag taagtgcggt gccggcgtgc tcgtgcagaa actaccgggg
      901 ccgtccaacg cggacgagtc cgagctcgag gcgacctgga atgatgcgca acggggaatc
      961 gagagtctga gcgccgccga cctcctcgag tgtcccgtgg aagagcttct cgagcgaggc
     1021 ttcgctgccc aggatctgcg ccttttcagg gggtcgccgg ttcagtttca gtgccgttgc
     1081 agcccggggc gagtggtcgg tctgctgcgc gccctcgggg cggacgaggt ccgcgacgtt
     1141 ctgcgcgagc agggggcggt caccgtgact tgcgagttct gccaccggcc ttacaagttc
     1201 gacgcagtgg acgtcgagtc tctgtttgca accggacccg attcgacgga agggtctccg
     1261 tcccttcatt gatccgcccc tgcatcgaat acgtcgggag gccgcccgat tgcccccagg
     1321 gtcggtcgat cggccgcacc ccgcttaagc gctagctaag ccacccgacc ccaccaccct
     1381 atttgaagta cgcgcattga cggacggtct gctatgctgc gattaaagaa ttgtgcaagt
     1441 atttaacaga tggcatcagg acccacagcg ctcgtcacct ggctgagagc gaccggcgaa
     1501 gtcagccgga tccggttgct gtctctgtgc agccgtcgcg acttcagtgt ttcggatctg
     1561 gcaagggcgc tcgggcagag tgagccgcgt gtctcgaggc acctgcgaat cctgtgtgag
     1621 gcgggcctgc tcgagagact gcggcaaggt cagtgggtgc attaccggct ggccacgcaa
     1681 gcggcagccg ccggcttcat tcagggcgtg cttgcgcagc tcgaccgatc cgatgcgcag
     1741 ctctcgcgcg atctcgagcg agcgcaggcg ccgcacgcga gtcgcgaaac ggcgcggccg
     1801 cgcgacggga gcgccgcctc cgagtctcgc ctcggacagg cgctgcaagg cttcgtggac
     1861 gccaacatgc cctcctctca gcagggatcc gctttgctgg tgggagtgga acatctcgaa
     1921 gtgctcgaga gcgcggcatc catggccgcc gactgtgtgg ctatcgctca ctcgcgccgc
     1981 gcagcccagg gcgcaagagc ctttgccgaa cgacgcggac tcaactgccg cgtgctgctc
     2041 gcatctgcgg ctcgtggcct gaatgatcgc gatatcggcg gccgtacgtt cgatggggtc
     2101 attctggatc acccggcggc cgtggacgca gacttcgcgg acctcctggc cagtgcgcgc
     2161 aatctcctgt cggcgaccgg caagctctgt ctgtttgagc gctacgagtc cctgcagatc
     2221 tcgcgcgagc gcgtcgtgga gcatccgctc gcgcggctgc gccgcttgct ggccgattcg
     2281 ggtctgacct gcgagcgtct gagtccgcta gaagccgatg gcgagcacgt gctggcggcc
     2341 gtcgccgtgc ccgctgccca tgcaggacta accaacctga ggcgcgcatg aatacgagca
     2401 accctcccca gttggcttgg cacgacccac acccctccat cagcgtgtcg ttcgagttct
     2461 tcccgccggc ggatgccgac atggaagtaa ctctgtggaa atctgtggag cgacttgcgc
     2521 cgctgacgcc gcgcttcatc tcggtgacct atggagcgga tggttcaacc cgtgagcgta
     2581 cgcacaatct cgtcacgcgc gtacagcggg aaacgacgct ggtcggtgcc ccgcatctga
     2641 cctgcatcgg cgcaacccag gccgagatcc tcgaaatcgc acgccgctac tgggacgaag
     2701 gcatccgcaa tgtggtcgca ttgcgcggcg atccgcctca ggggcaggcg ggttatcggc
     2761 cgcatccaga tggatttgct tacgccgcgg atctcgtcgc cggcctgaag cgcgtcgccg
     2821 acttcgacgt gtcggtcgca gcctatcccg aagttcatcc cgaggcgcca tccgccgagt
     2881 tcgatctgga caatctgaag cgcaaggtgg atgcgggtgc ctcacgcgcc atcactcaat
     2941 ttttctacga ctccggcgtc tatcttcgtt tccgggaccg ctgcgcagct gcgggcattc
     3001 aggcctgcat cgtgcccggc attctgccga tcacgcggtt tccgcaagtg ctgaagttcg
     3061 ctgcgcgctg cggcgcgagc attccggagt ggctgaaaga gcgcttcgag ggcctggacg
     3121 aggatgccga gacccgccga ctgatcgcag caagtgtcgc catcgaacag gtccaggcac
     3181 tccagcggga aggggtgcga gagtttcatt tctacacatt gaatcggtcc gagctgacct
     3241 acgccatctg tcatgcgctg ggtgtacgga ctgccaacca gcgagcggta gcatgagcat
     3301 ttgcccacac gaggaacaca cttcgattcc gtggcgcgcg gatgcgcgca aggatggcgc
     3361 cgcggcccgg gccgctgccg ttcccttcgg catggaatcg gtggatgaga ccgcgaggcg
     3421 ggagcgcatt tcacgcctga aagggctgct ggacgagcga atcatcgtgc tggatggtgc
     3481 gatgggcacc atgatccagc agcacaagct cgatgaacgc gccttccgtg gcgagcgctt
     3541 ccgcgcctac ggtcgggatc tgcgcggcaa caatgacatt ctgtgtctca cgcatcctga
     3601 catcgtgagt gagattcatc gggcgtatct ggaggcaggc gcggatctca tcgagaccaa
     3661 cacattcaac tccacggcca tctcgcaggc ggattacgga accgaggcac tggtgccgga
     3721 gctcaactac cgggcagcgc agctcgcgcg tgcggcggcc gatcagttcg cgcatgcatc
     3781 cgggcgagcg gctttcgtcg cgggcgcact cggcccgacg agccgcatgg catccctctc
     3841 gccggatgtg aacgatccgg gctaccgaag tgtgacgttc gatcagctcg tcgcaaccta
     3901 tacggaagcc gcgagaggcc tgctgcttgg cggggtcgat gtgctgctca tcgagaccat
     3961 cttcgatacg ctgaatgcca aggcggcgat ctacgcagtg cagggcctgc tcgatgagct
     4021 gtccctgccg gtgccgatca tgatctccgg cacgatcacg gatgcctcgg gccggctgct
     4081 gtccgggcag acgccgcaag gcttctggaa ttccatccgc catgccagac cgctcgcggt
     4141 ggggctcaat tgtgctctcg gagccagcca gcttcgccag tacgtccagg agctgggccg
     4201 ggtggccgac acctacatct gcgcctatcc gaatgccggt ctgccgaacg ccttcggcga
     4261 atacgatgaa acaccggagg aaacggcagc catcctcaag gactatggcg cttcgggtct
     4321 tctcaacatc gtcggtggct gctgcgggac gacgccggcg catatccggc tgatgcgaga
     4381 tgcgatgggg tcattcccgc gccgcgagat tcccgcgagc gtacccaagt gccgactgtc
     4441 tgggctcgag cctctcgaca tcgatgctga cagcctgttc gtgaacgtcg gtgagcgcac
     4501 caacgtcacc ggttccgcga agttccgcaa gctgatcgag gcgggcgact acgccgccgc
     4561 gctcgaggtc gcccggcagc aggtcctcaa cggcgcgcag atgatcgatg tcaacatgga
     4621 cgagggcatg ctggattcgg aaagcgccat gacgcgcttt ctgcacctgg tcgctgccga
     4681 gcccgacatc gcgcgcgtgc cggtcatgat cgactcctcc aagtggtcgg tcatcgaggc
     4741 cggattgaaa tgcgtgcagg gcaagccggt cgtcaactcg atcagcctga aggagggcga
     4801 ggaggctttc atcgagcatg ctcgcaaggt acgccggtac ggcgccgcga tggtcgtgat
     4861 ggctttcgac gagcagggtc aggcggacag catcgagcgc aaggtggcga tctgcaagcg
     4921 ctgttacgac attctcacga cccgcgcgga tgtgctgccg gaagatatca tcttcgatcc
     4981 caacatcttc gc
//
LOCUS       DASRHN010000176        17186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1539631, whole genome shotgun sequence.
ACCESSION   DASRHN010000176 DASRHN010000000
VERSION     DASRHN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539631"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFB37_06780"
     CDS             complement(<1..698)
                     /locus_tag="VFB37_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZO22195.1"
                     /translation="MKRQPAWAAFIAASLVSCGGGDPAVTPTGVAAGSTGCTGTCATA
                     SSLLTVTDVERVIAQGVAEAQARNVHATIGVVDRVGNVLAVYRMGSPASRSVQIASQL
                     DSAGSAVVHTGLEGLRLPVPGALGGFNLDELAAIAKAITGAYLSSEGNAFSSRTASQI
                     VQQHFDPGTHGAPSGPLFGVQFSQLACSDLVMSSAGSAATVGPQRSPLGLSADPGGFP
                     LYKEGTVVGGVGVLA"
     gene            complement(695..2410)
                     /locus_tag="VFB37_06785"
     CDS             complement(695..2410)
                     /locus_tag="VFB37_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZO22196.1"
                     /translation="MDVLLRQLRTGPDGATEYQDTELSAEVLSLGSAADCTIQLLGTG
                     VEAHHAALRSRGSDFSIECQRPATVAVNGRRTRSATVKAGDLLEIGAHRLRVLEAPAG
                     FDLGLEWEPDPQVAPSAYERAFRTELSMTWLSRRGAAWILAAIILLAALLIPYGSLVR
                     QRTGAAVPLLVPSDRMWSAGPLTPAHQLAAGQRCGSCHQQLFVHVRDAACRECHRGIG
                     DHVEPAHLAQTQLGPAQRCAQCHQEHHAPVTGLVVRDNSLCVDCHAKSDVAFGKLKVQ
                     PVSGFSQQAHPAFTVSLIKPSDKLDESGQVQWAVRREPITAAREQSNLKFSHQQHLDP
                     SQVTRASDRGALGCSDCHVLDADGEHFVPITMQRSCASCHELTFDPQAPERQLPHGKP
                     RDAILLIQDYFVRNAVLPVAPTSGFHRRRMPEEQQSEAAVCNGPPLSCAMQRARAEIE
                     NQFTGRGCVSCHEVKDQHTAELLERFRVLPVRLTRDYFPKVHFSHRLHAVQKGKSGDA
                     ACVSCHAVKTSRSSADLFIPNLPKCLECHSERLAVDRVTLQCSSCHSYHPITTYDRLR
                     EADAQ"
     gene            complement(2414..3313)
                     /locus_tag="VFB37_06790"
     CDS             complement(2414..3313)
                     /locus_tag="VFB37_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22197.1"
                     /translation="MIHQNILRFRNGRYAWWALGLTVLSVIIYATQGGRERPGGGTWQ
                     GYALGTLGALLIVWLALLGIRKRSYRSTLGSVQGWTSAHVWLGLALVLIATLHCAGLF
                     GWNVHTLAYALMCLVIVSGIFGVYVYLSMPRQLSANTGGLSRAALFAELFGLDRQGRE
                     LARACDPAIGNAVRSSIERTSLGGGVLRQLLAIDRSLFMRTDADADHARASTSVVRNP
                     DQQAVIDYVARRVPQAAKRAEAATLQALMVLLSRRQAVLRRIRRDIQLQGWLQLWLYI
                     HVPLTIALIVALIVHILTTFMYW"
     gene            complement(3310..5865)
                     /locus_tag="VFB37_06795"
     CDS             complement(3310..5865)
                     /locus_tag="VFB37_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZO22198.1"
                     /translation="MPAAQPTEYRLAIVGAGPAGLSSAARAAQIDAREGRRVPSYVLL
                     EGSSKLAKTIQRYQKGKHVMAEPGFLELRSDLKFAAGSRESILETWGADASRLGVNVR
                     YGAEVKKISGQRGAFLLQLGGGETIRAATVVLAIGLEGNPRRLGVPGEQCEEVQYQLD
                     DPGEYLDETILVVGAGDAAIENALALSAQNDVWIVNRGKEFSRAKDANLSAVLAAISD
                     PQRRLACYYETRPKAITHSSTAPRPLTVTLETPAGERIVQCHRIIARLGSDPARAFVE
                     STGIRFPGPQREAIPALSREYESNVPGLYIVGSLAGYPLIKQAMNQGYDVAEYAYGNR
                     IKPADYPLLEWQFCGLPFQRETEDLLERFKSIVPMFRELNALAFRELVIESEVIAAYP
                     EGPEHAEARRKVDSVRQSIANKPGQPRVTRVIREGEVIYDTGEFGTSFFTIAYGEVTL
                     QTSGPTPVTTQLKLGEFFGEMSLLSGRPRTERAIAGPGCILMETPRRTMIKLMSSNES
                     VRNGIDWIFVVRELQRVFAPQASARELRDVAAQVSVRAFKAGELIFSEGEQGASLFIV
                     RSGGVTLSRRRNAHDLMVAQVPAGQLVGEMSLMGDPQRRETARATVASELIEVQRQEF
                     LRLSKRADAPLELLQRAASAHVAESARMEVRPEASSIMNFLMAQGLGEATNALIIDES
                     LCIGCDNCEKACAETHGGIARLDRKAGASFAQVHVPISCRHCEQPHCMKDCPPNAIKR
                     SANGEVFIDETCIGCGNCQVNCPYGVIRMTYEAPRKPGLLSWLLFGAGPGVGEDKDYE
                     PTSTGKAKGKKATKCDACGGISNGPACVQACPTGAAIRVGPDQYLDLADQRRR"
     gene            6259..8961
                     /locus_tag="VFB37_06800"
     CDS             6259..8961
                     /locus_tag="VFB37_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HZO22199.1"
                     /translation="MRALSLSVSCRLLACICSAILSAAAIGATSKDSTEIATRAQIAA
                     SNLADALVVDCQLPGKLQQLGGMRTYLTPGALLRLPAIDCRTRGGEYTVGDLASGTLS
                     LNRWMPLAEKDNVEAEYYVARIYANGMDGVPVDYAKAAQWYQRAAQKKYAPAMQELGY
                     LYEQGLGVQQDALAGLNLQREASGLGNELDYASKLTANREEAAKQIAAVTDQLDAANG
                     AVQDLRDQLRQVNERLLQNTNQLDRDQVRLADLHSQLEAAKRDGSGQSGARIEQLQQQ
                     LAADEKALAEKQQAVGTLSANLVVQQTQLSEQLARSQAENSHLNDLLASGRSETEALR
                     ARLAQSEQRLNQTQQELTAVRADYLRETSSLAARSEELQKLRAHGGDGAQALLAAKQR
                     ELDQQQAQIKSLQTQVEGMRAQASASGASASEATTRKQELEASLQALRSQYTQQQQQL
                     QDQRAQFAHLQSQSKADRDALAAQMTAGMTAKAAQLEEQQKRIAALQLEANQLREAYN
                     HDREAHQQQAASSTAEAQRDRDALRAAQEKMAKQQDTVEHLEMELAARQLELVRARQS
                     SAQQPAADTQANQKRIAALETDVRDRDKQISDLRFELAKAGNPASPPPPVAGNVAYRM
                     AQASGPGSADPEALMRMVRGLGPANYHALIIGNSNYLYMEKLSTPVSDARDIATVLRT
                     RYGFDVKLLTDATRDQIMAAMNEYARTLTDSDRLLIYYAGHGGTKIDPPERAFWLGVD
                     ANPELRGSWLSAQMVADAVGEIHARHILLVADSCFSSVITHPTSTTVARSDDEHSTAI
                     RWSKAARMVLTSGENEPVVDKSAADPAHSLFASQFITVLRQNDILLSGEMLAHELSSR
                     MSGFAARMGLKQTPTYSNLQDPAHKFGDFFFLPVRTTQVAALMR"
     gene            9032..10684
                     /locus_tag="VFB37_06805"
     CDS             9032..10684
                     /locus_tag="VFB37_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HZO22200.1"
                     /translation="MSTNSFRTIAPAGMLAALCALAAPVHAQLPPPPPNATALQAAVF
                     NAVAKMCVLDLDLPKNPPPANPQVNDLHDQCHAIAVANLNVMPADAGPIFGPAPAGAL
                     GALQQVSGNEVSTQGALATRVSAGQFGNITGRLNALRLGSAFSLSQGLSARTDSSAPG
                     GSSLASLAPQTFYFDRSAVDPNAQEDQLSRGTLLPVGNYQNPGALVGTTYSDSYRYLA
                     DPAGGGGLGGNVPSPWGLFVQGSYNSGRHDQTQNEDPFDFHASSVTAGIDYNFGGAVL
                     GASLGYDDYDAGFNNTGVNSTGGSAQVKGTSGSLYGAWFGQNWTLNGIATYGKLTTNL
                     SRTVKYTVTYNTALDPQADINDPGCAGAATCTVSVNRTLDGNPDGRSLAVGATAGYQT
                     QAASWDVMPSLSVNYRRASFDSFAEHDPNDPNDGLPLFFNDQTVESLRSILGLDLSKP
                     VSTSFGVVTPILRVEWDHEFKTSVRMIDAHYVFDPSCKNGVCDSNFLLPTDPAVGNYG
                     VAGVGLSVTLAQRVQAFVYDEILFGYQDYHSNAVTIGLRGQF"
     gene            10931..11752
                     /locus_tag="VFB37_06810"
     CDS             10931..11752
                     /locus_tag="VFB37_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22201.1"
                     /translation="MERTIAFLSKRSDPDSLAAAGVLLLRTQADQGLLLLARATAAAP
                     SRVDLAWLQIQVCLRIPSCASQPMEVHLRTLDPSNGAGWLGALNRAESAGDASAKGAA
                     LLHISHADRVAIYWTTLISRLTPAVARAGVLPLPQAEVAVIGMLAAADIPPYKIIADY
                     CIRGQLADDQDVALCRGIGRALERGDTYATELIGIAIARHVWPENSAEWKAAAYARSV
                     YEYRTNLLAHLEAHVALDRHAAEGYLALCAKYPREQDVARARLLEAGEKPDPPAQ"
     gene            11922..12350
                     /locus_tag="VFB37_06815"
     CDS             11922..12350
                     /locus_tag="VFB37_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO22202.1"
                     /translation="MPDITGIRVLFIAGCGPIVRDGPASRKLYGQALGISFKEEAGGY
                     LHTEALSGAKSFALWPLAEAARSCYGEPAWPGDVPVPQAWIEFDVDDIDKATAELESR
                     GYPMLVRNKKEPWGQIVSRFLSPEGLLVGITFTPQMREQK"
     gene            complement(12402..13385)
                     /locus_tag="VFB37_06820"
     CDS             complement(12402..13385)
                     /locus_tag="VFB37_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22203.1"
                     /translation="MLSLLLAARLSHFAAAQDAPAPTRHLISELTPIARIHLGKTADW
                     VAVTGDTVWIGTTGPDGVARIDPRSNTRTAAICLRGKPCAGLAVGFGGLWVPLCGEPN
                     ALARVDLLTSQVVMVPAAGPAEREGGITVSRDSVWLVVDGRATLARIDPSSLLIKQRI
                     SLARGSLNVAFSGGRIWVTRPTGAEVTVVDADKGAVIGTVPTGPRPRFLDAGGQSVWT
                     LNQGDGTLTRINARSRRADFSSSLQTPGHGGDIKIAHGIVWTSMAKVPLSATDEVTGR
                     VLCQWVGPGGDALGVGHDAIWLTDYEAGDVYRYDLEDVLDHCPSARRDRKP"
     gene            13593..14267
                     /locus_tag="VFB37_06825"
     CDS             13593..14267
                     /locus_tag="VFB37_06825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO22204.1"
                     /translation="MTIRDVAYWLRSARADARLERFRQQGPGGSPFDHLYAETDDPFG
                     AELPQYRYQQRKYACLLSMLPQRRYRNILDVGCGLGTFTRKLAPFGEQVLGTDISGEA
                     ISQARRLSFGHSNITYAQRDMLDAPATESGFDLIIIADTLYYINPRTPVALQALAQTF
                     ASKLTPGGLILIVNHFFFGVDAASRQTREIHDVFRGAPSLECIAEIRRAFFLATLLQR
                     AEVWSG"
     gene            complement(14256..14329)
                     /locus_tag="VFB37_06830"
     tRNA            complement(14256..14329)
                     /locus_tag="VFB37_06830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14295..14297),aa:Gly,seq:ccc)
     gene            complement(14383..14796)
                     /locus_tag="VFB37_06835"
     CDS             complement(14383..14796)
                     /locus_tag="VFB37_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO22205.1"
                     /translation="MKTPKPFTRSACAIANSLDLVGDKWSLLVVRDLMHGKQTYGELA
                     DSPERIPTNILADRLERLEGAGIITSAPYQERPVRYAYALTPKGKALGEVLLAFVRWG
                     KQYIPGTITLSENPRAGAGEVSAAGSSRLRAKRSR"
     gene            14903..15562
                     /locus_tag="VFB37_06840"
     CDS             14903..15562
                     /locus_tag="VFB37_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22206.1"
                     /translation="MTALVLPQPTESPSGQRPVRLAVSIALLAWFLLVVALGAVGAFV
                     GPPGTPPVPIALGVATPLVIFFLLLRFSTSFREFVLSLDLRLVAGIQAWRWAGMGFLF
                     LYAYKVLPGWFAFPAGLGDMAIGATAPWMILSLARQPGFAATSAFVRWNLLGILDLAV
                     AVSIGAVSAIFATGNPGELSAAPMASLPLLLIPAFLVPSFLMLHTTALMQSRQLRRTR
                     M"
     gene            complement(15644..16579)
                     /gene="bla"
                     /locus_tag="VFB37_06845"
     CDS             complement(15644..16579)
                     /gene="bla"
                     /locus_tag="VFB37_06845"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HZO22207.1"
                     /translation="MRGCRLKTGAKVQMNMLLTRRAALISGLALLRSLSTAARGEGAT
                     QARGGPGDLQQRITELEKHSGGRLGVAMLDTASHARLHYRAEERFAMCSTFKFVAAAA
                     VLQRVERGEEKLDRWIVYGPEDLLEYAPVMREHVQEHGMRLSDVCAAAVQWSDNTAAN
                     LMLRVIGGPAGFTQYCRSLGDRITRLDRIEPELNRVAAGDGRDTTTPAAMLSLLHSLL
                     LGKALADSSRQQLESWMVNATVGKDSIPAGLPPEWRIGHKTGSGRDESNDVLIAWPPG
                     RAPVLITAFYSRPSGSPQERDGVLREVGRIVSAAL"
     gene            complement(16646..>17186)
                     /locus_tag="VFB37_06850"
     CDS             complement(16646..>17186)
                     /locus_tag="VFB37_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22208.1"
                     /translation="GFINAGLLHFQGITASGDYTLDLPWNAGSLEARAAYLDTRRLVL
                     QVGSAAPLNEAGELNAGGFPTTPKGRGTASLTWTKGPLSWYWQAQYFSGMNFNNLNTP
                     TSQNILRVNPWWLINSTITFNVTTNFQARLVVDNVFDKEPPFPALAAGTGGNFAGATS
                     LYFPGILGRSYLLAVDYRF"
BASE COUNT         3127 a         5602 c         5414 g         3043 t
ORIGIN      
        1 gccagaaccc cgacaccgcc gacgaccgtg ccctctttgt agagggggaa cccgcccgga
       61 tccgctgaca gtccgagcgg cgagcgctgc ggtccgaccg tcgccgcgct gccggcagag
      121 gacatcacca gatccgagca ggcgagctga ctgaactgga cgccgaacag gggaccgctc
      181 ggcgcgccgt gcgtcccggg atcgaagtgc tgctgcacga tctgactggc cgtgcgcgag
      241 gagaaggcgt tgccttcgga ggacagatag gcgccggtga tggccttggc gatggccgcc
      301 agctcatcca ggttgaagcc gccgagcgcg ccaggcacag gcaggcgcag gccttccaga
      361 ccggtatgca cgaccgcgct gcccgcggag tcgagctggc tggcgatctg tacagaacgg
      421 ctggcgggtg agcccatccg atagaccgcg agcacgttgc ccacccggtc gacgaccccg
      481 atcgttgcgt ggacgttgcg cgcttgcgct tcggcaactc cctgagcgat cacccgctcc
      541 acatccgtca cggtgagcaa cgagctcgcc gtggcgcaag tgccggtgca gccagtcgag
      601 ccggcggcaa cacccgtcgg ggtgacggcc ggatcgccgc ccccgcacga gacgaggctg
      661 gccgcaatga atgccgccca cgctggctgt cgtttcattg tgcgtcggcc tccctgaggc
      721 gatcgtacgt tgttattgga tggtagctgt gacagctcga gcactggagc gtaacacgat
      781 ccacggcgag tcgttcactg tggcactcga gacacttcgg gaggttgggt atgaagagat
      841 ccgcgctcga gcgggatgtt ttcactgcat gacaggacac gcaggccgca tcaccgctct
      901 tgcccttctg gactgcgtgc aggcggtgac tgaagtgcac cttcgggaag tagtcgcgtg
      961 tcagacggac cggcagcacc cgaaagcgct cgagcagctc cgccgtgtgt tgatccttga
     1021 cctcgtgaca gctcacgcag ccccggcccg tgaactggtt ttcgatctcc gcccgagcgc
     1081 gctgcatcgc gcagctcagc gggggcccgt tgcaaaccgc cgcctccgac tgctgttctt
     1141 ccggcatacg ccgccggtga aatccgctgg tgggcgcgac cggcagcact gcattgcgca
     1201 cgaagtagtc ctggatgagc agaatggcat cccggggctt gccatgggga agctggcgct
     1261 caggtgcctg cgggtcgaag gtcaactcat ggcaggaagc acacgagcgc tgcatcgtga
     1321 tcggcacgaa atgctctccg tccgcatcga gcacatggca gtccgagcat cccagggcgc
     1381 cgcggtcgct ggcgcgcgtg acttgactcg ggtccagatg ctgttggtga gagaatttca
     1441 ggttcgactg ctcccgggca gcggtgatcg gctcgcgccg gaccgcccac tgcacttgcc
     1501 cgctttcatc cagcttgtcc gacggcttga tgaggctcac cgtaaaggcc ggatgcgcct
     1561 gctgcgaaaa tccgctgacc ggttgcactt tgagcttgcc gaatgcgaca tcggactttg
     1621 cgtggcagtc cacgcacagc gagttatcgc gcaccacgag gcccgtgacg ggggcatgat
     1681 gttcctgatg gcattgggca cagcgctgtg cgggaccgag ctgggtctgg gccagatggg
     1741 cgggctcgac atgatccccg atgccgcgat ggcattcgcg acaggccgca tcccgcacat
     1801 ggacgaagag ctgttgatga cagcttccgc aacgctgccc ggctgcgagt tgatgagcgg
     1861 gcgtgagcgg gcctgcgctc cacatgcgat cgctcggcac cagcaggggc acggcggccc
     1921 cggtgcgctg tcgaaccagg ctgccgtagg gaatgagcag cgcggccagc aggatgatcg
     1981 ccgccaggat ccaggcggcc ccccgtcgcg agagccaggt catcgagagc tcggtgcgga
     2041 aggcgcgctc ataggcgctc ggcgctacct gcggatcggg ctcccattcg agccccagat
     2101 cgaagccggc cggagcctcc agcacgcgca gccgatgcgc gccgatctcc agcagatcac
     2161 ccgccttcac cgtcgcagag cgagtgcgtc ggccgttgac cgctaccgtc gccggccgtt
     2221 gacactcgat cgagaagtcg ctgccacgcg agcgcaaagc ggcgtggtga gcctccacgc
     2281 cggtaccgag cagctggatc gtgcagtccg cagcgctgcc cagggacaga acttccgcag
     2341 acagctcggt gtcctggtat tccgttgcgc catcggggcc ggtgcgcaac tgtcgcagca
     2401 gcacgtccac gactcaccag tacatgaagg tcgtgaggat gtgcacgatg agcgccacga
     2461 tgagcgcgat cgtcagagga acgtgaatgt acagccacaa ctgcaaccaa ccctgcagtt
     2521 ggatgtcgcg tcggatccgt cgcaacaccg cctggcggcg ggacagcagg accatcagcg
     2581 cctggagagt cgccgcctca gcacgcttcg ccgcctgcgg cacgcgtcgc gccacgtaat
     2641 cgatcaccgc ttgctggtca gggtttcgga cgacactggt gctcgcgcgc gcatgatccg
     2701 cgtctgcatc ggtccgcatg aacagcgatc gatcgatcgc cagcagctgt ctcaacacgc
     2761 ctccacccag ggaggttcgc tcgatgctgc tgcggaccgc attgccgatg gccggatcgc
     2821 aggctcgggc cagctcgcgc ccctgacggt ccaggccgaa gagctcggcg aacagggctg
     2881 cgcgggacag cccgccggtg ttggccgaga gctgccgggg catggacaga tagacataca
     2941 ccccgaagat gccactcacg atcaccaggc acatgagcgc gtaggcgagg gtgtgcacgt
     3001 tccatccgaa cagaccggca cagtgcagag tggcaatcag cacgagcgcc agtccgagcc
     3061 agacatgggc cgaggtccaa ccctgcaccg agcccagcgt ggagcgataa ctgcgcttgc
     3121 gtatcccgag cagcgcgagc cagacgatca gcagcgcacc gagggttccg agcgcatatc
     3181 cttgccaggt accgccaccg ggacgctcgc ggccaccctg cgtcgcgtaa atgatgacgg
     3241 acagtaccgt gaggccgagg gcccaccagg cgtagcgccc attgcgaaag cgcaggatgt
     3301 tctggtggat catcggcgtc gctgatcggc aaggtccaga tactggtcag gtcccacgcg
     3361 gatcgccgca ccggtgggac aggcctgcac gcaagccggc ccgttgctga ttccgccaca
     3421 ggcatcgcac ttcgtggcct tcttgccctt ggccttgccg gtggacgtcg gctcgtaatc
     3481 cttgtcttct cccacgcctg gccccgcacc gaacagcagc catgacaaca aacccggctt
     3541 tcgtggcgcc tcgtaggtca tgcggatgac gccgtacggg cagttcacct ggcagttgcc
     3601 gcatccgatg caggtctcat cgatgaacac ttcgccattg gccgagcgct tgatcgcatt
     3661 cggcgggcaa tccttcatgc agtgcggttg ctcgcagtgg cggcacgaga tcggaacgtg
     3721 cacctgcgca aacgacgccc ccgccttgcg atccagccgc gcaatgccgc cgtgcgtctc
     3781 ggcacacgcc ttctcgcagt tgtcacagcc aatacacaac gattcgtcga tgatcagcgc
     3841 gttggtggct tcgccgagcc cctgcgccat caggaagttc atgatggagc tggcctcggg
     3901 acgcacttcc atccgcgcac tttcggcaac atgagcgctc gccgcgcgct gcagcaactc
     3961 caggggcgca tccgctcgct tcgagaggcg cagaaactcc tggcgctgca cttcgatgag
     4021 ctcggaggcc accgttgcgc gggccgtctc gcgccgctga gggtcgccca tcaaggacat
     4081 ctcacctacg agttgacctg cggggacttg cgccaccatc aggtcatggg cgttgcgccg
     4141 acgggacagc gtgacgccgc ccgaccgcac gatgaacagg ctcgccccct gctccccttc
     4201 cgagaagatc agctcgccgg ccttgaaagc tcgcacgctc acctgggctg cgacgtcgcg
     4261 caattcgcgt gcgctcgcct gaggggcaaa aacccgctgc agctcgcgca cgacgaagat
     4321 ccagtcgatt ccgttgcgca cgctctcgtt ggagctcatc agcttgatca tcgtgcgccg
     4381 cggggtctcc atgaggatgc atcccgggcc ggcaatcgcc cgctcggtgc gcggccggcc
     4441 cgagagcaag ctcatctcgc cgaagaactc cccgagcttt agctgtgtcg tcaccggcgt
     4501 ggggcccgag gtctgaagag tcacctcgcc ataggcgatc gtgaagaacg aggtgccgaa
     4561 ctcgccggtg tcatagatca cctcgccctc gcggatcacg cgtgtcacgc gcggctgtcc
     4621 gggcttgttg gcaatacttt gccgcacaga gtcgactttg cgacgcgcct ccgcgtgctc
     4681 gggtccttcc ggatacgcgg cgatcacttc gctctcgatg acgagctcgc gaaaagcgag
     4741 ggcgttgagc tctctgaaca tcgggacgat ggacttgaag cgctcgagca gatcttccgt
     4801 ctcgcgctgg aacggcaacc cgcagaactg ccactcgagc agcgggtagt ccgccggctt
     4861 gatgcggttg ccgtaggcat actcggcgac gtcatacccc tgattcatcg cctgcttgat
     4921 caggggatag ccggcgaggg agccgacgat gtacaggccg gggacgttgg actcgtattc
     4981 gcgcgaaagc gccggaatgg cctcgcgctg cggacctgga aagcggatac ccgtggattc
     5041 gacgaaggcg cgggccggat ccgagcccag gcgcgcgatg atgcgatggc actgcacgat
     5101 gcgctcgcca gccggggtct cgagcgtgac cgtcaagggg cgcggcgcgg tgctcgagtg
     5161 cgtgatcgcc ttcggccgcg tctcgtaata gcaggcgagg cggcgctgcg gatcgctgat
     5221 cgcggccagg accgcggaga gattggcgtc cttggcgcgg ctgaactcct tgccacgatt
     5281 cacgatccag acatcgttct gcgcgctcag ggccagggca ttctcgatcg ccgcatcccc
     5341 ggcgcccacc accaggatgg tctcgtccag gtactctccg ggatcatcga gctgatactg
     5401 aacttcctcg cactgctctc cgggcacgcc cagccggcgg ggattgcctt ccaggccgat
     5461 ggccagcacc accgtcgccg cccggatcgt ttcgccgcca ccgagctgca gcaggaaagc
     5521 cccgcgctgg ccgctgattt tcttgacctc ggccccgtag cggacgttga cgccgagccg
     5581 cgaggcatcg gcaccccagg tctccaggat cgactcgcga ctgcccgcgg caaatttgag
     5641 atcgctacgc aactcgagaa agcccggctc ggccatcacg tgcttgccct tctgatagcg
     5701 ctggatggtc ttcgcgagtt tcgaagagcc ctccaggagc acgtagctgg gcacgcgacg
     5761 gccctcacga gcatcgatct gggctgcgcg cgcggcggag ctcagtccgg ccggcccggc
     5821 cccgacgatg gccaggcggt actcagtggg ttgggcggcg ggcatgggat ctcaataaag
     5881 acttgaacat catgtagtta tgacgtcagt gacgttatgt tgtgatgaca gcgcgctcga
     5941 gcccctgtgc cgccgccgtt ctcagtacgc agcccgttgc gatccggatg cgacagcgag
     6001 cgcccgctgt tgttttggac cccccctgcg ccgtcgcact ctgtaccaca ccgattgcag
     6061 gcagggaagc ccgcggcggc gtgcaaatca actggggcgc gctcatgccc cccgcgcgaa
     6121 cctcgaggct gcgaatattc gcgcccgatg tccggttttc cgccgcttct gcgttacttg
     6181 ccgataggca acttgcgacg gtcgtgcgag atggctcaga tccctttaaa ctgaccggcg
     6241 agcggatccg gggtcatcat gcgagcgctc agcctctcag tctcatgcag gctcctggcc
     6301 tgtatctgca gcgcaattct cagcgccgca gccatcggtg ccacctcgaa ggattccacc
     6361 gagatcgcca cgcgcgctca gatcgccgcc tccaacctgg ccgatgcgct cgtcgtggat
     6421 tgccagcttc cgggcaagct gcagcagctg ggtgggatgc gcacgtatct gacacccggc
     6481 gccttgctgc gcctgccggc catcgactgc cgcacccgag ggggtgagta cacggtcggg
     6541 gacctggcga gcggaacgct ctcgctcaac cgctggatgc cgctggccga gaaggacaat
     6601 gtagaagccg aatactacgt cgcgcggatc tacgccaatg gaatggacgg cgttccggtc
     6661 gactatgcga aagcagccca gtggtatcag cgcgccgccc agaagaaata cgcgccggcg
     6721 atgcaggagc tcggctacct gtatgaacag ggactcgggg ttcagcagga tgcactggcc
     6781 ggcctgaatc tgcagcgcga ggcctccggg ctcggcaacg agctggacta tgcgtcgaag
     6841 ctgacagcca atcgcgaaga ggcggccaag cagatcgcgg cggtgaccga ccagctcgat
     6901 gccgccaatg gcgcggtgca ggacctgcgt gatcagctcc ggcaggtcaa cgagagactg
     6961 ctccagaata ccaaccagct cgaccgcgat caggtgcgac ttgccgacct gcactcgcag
     7021 ctcgaggcgg cgaagcgcga cgggtccggc cagagcggcg cgcgcatcga gcagctgcag
     7081 cagcaacttg ccgccgatga gaaggcgctc gccgagaagc agcaggcggt cggtacgctc
     7141 tcggcgaatc tcgtcgtgca gcagacgcag ctgtccgagc aactcgccag gtcgcaggcc
     7201 gagaactcgc atctgaacga tttgctcgcg agcgggcgca gcgagaccga ggcgctgcgc
     7261 gcccgcctcg cccagtccga gcagcggttg aatcaaactc aacaggagct gaccgcagtc
     7321 cgtgcggact atctgcgcga gaccagctcg ctcgccgcgc gcagcgagga gctgcagaag
     7381 ctcagggcgc acggggggga tggcgcgcag gcgctgctcg ccgcgaagca gcgcgagctc
     7441 gatcagcagc aagcgcagat caaatcgctc cagacgcaag tcgaagggat gcgggcgcag
     7501 gcgagcgcct ccggtgccag cgcctccgag gcgacgacgc gcaagcagga gctggaagcc
     7561 tcgctgcagg cgctgcgctc tcagtacacg cagcagcaac agcagctgca ggatcagcgc
     7621 gcgcaattcg cgcatctgca atcacagtcg aaggcggacc gcgatgcgct tgccgcgcag
     7681 atgaccgcgg gcatgaccgc gaaggcggcg cagctcgaag agcagcagaa gcgcatcgcc
     7741 gctctgcagc tcgaggcgaa tcagctccgc gaggcttaca accacgaccg cgaggcccat
     7801 cagcagcaag cggcctcttc cactgccgaa gcgcaacggg accgcgatgc gctgcgcgcc
     7861 gcgcaggaga aaatggcgaa gcagcaggac acggtcgagc atctggagat ggagcttgcg
     7921 gcgcggcagc tcgagctggt gcgcgcgaga cagagctcgg ctcagcagcc ggccgccgac
     7981 acgcaggcca atcagaagcg catcgccgcg ctcgaaacgg atgtccgcga ccgcgacaag
     8041 cagatctccg acctgcgttt cgagctcgcc aaggccggca atccggccag tcctccgccg
     8101 cccgtggctg gcaatgtggc ttatcggatg gcccaggcct cgggccccgg aagcgctgat
     8161 cccgaggcgc tcatgcgcat ggtgcgcggc ctgggacctg cgaactatca cgctctcatc
     8221 atcggcaaca gcaactacct gtacatggag aagctgtcga cacccgtcag tgacgcacgc
     8281 gacatcgcga ccgtgctgcg gacgcggtac ggcttcgatg tgaagttgct gacggatgcg
     8341 acccgcgatc agatcatggc cgcgatgaac gagtacgcac gcaccctgac cgacagcgac
     8401 cggctgctca tctattacgc aggccatggc gggaccaaga tcgatcctcc cgagcgggcc
     8461 ttctggctgg gcgtggatgc caatccggag ttgcgcggca gctggctgtc cgcccagatg
     8521 gtggccgatg cggtcggaga gattcacgcc cgccacatcc tgctggtcgc cgactcatgc
     8581 ttttcaagtg tcatcacgca tcccacctcc acgacggtgg cccgatcgga cgatgagcac
     8641 agcacggcga tccgctggag caaggctgca cgcatggtgc tcacttcggg cgagaacgag
     8701 ccggtggtgg acaagagtgc cgcggatccg gcgcattcac tgttcgcgtc gcagttcatc
     8761 acagtgctgc gacagaatga catcctgctc tccggagaga tgctggcgca cgagttgagc
     8821 agccgcatgt cggggtttgc ggcgcgcatg gggctgaagc agacgcctac ctactccaac
     8881 ctgcaggatc ccgcgcacaa gttcggcgat ttctttttcc tacccgtaag aaccacccag
     8941 gtggcggccc tgatgcgata gtccaacgct gcacgcgcgc tcgcttgggc ggtcgcgact
     9001 tccgaaaaaa catcaacagg agagggatcc agtgtctacc aactcgttcc ggacgatcgc
     9061 acccgcaggc atgctggcag cactctgtgc gctggccgcg ccggtgcacg cgcaactgcc
     9121 acccccaccg ccgaacgcga cagcgctgca ggcggcggtg ttcaacgcgg tggcgaagat
     9181 gtgtgtcctc gatctcgacc tgccgaagaa tccgccaccg gccaaccctc aggtgaacga
     9241 cctgcacgac cagtgtcacg ccatcgccgt ggcaaacctc aatgtcatgc ctgcggacgc
     9301 gggccccatc ttcggaccgg cacctgcggg cgccctgggc gccttgcaac aggtgtccgg
     9361 aaatgaggtg tccacacagg gcgcactcgc tactcgagtc agtgccggtc agttcggcaa
     9421 tatcaccgga cgactgaatg ccctgcgcct gggctccgca ttctcgttgt cgcaggggct
     9481 ttcggcccgc actgacagct ccgcgcctgg gggctcttcc ctggcctctc tagccccgca
     9541 gaccttctac ttcgaccgct cggccgtgga tccgaacgcg caggaggacc agctgtcgcg
     9601 gggcacgctg ctgccggtgg ggaactatca gaatccgggc gcgctggtcg gcacgaccta
     9661 cagcgacagc taccgctatc tggccgatcc ggcgggcggc ggtggtctgg gcggcaacgt
     9721 gccgagtccc tggggcctgt tcgtgcaggg aagctacaac tccggacgcc atgaccagac
     9781 gcagaacgag gacccgttcg atttccatgc ctcgagtgtc acggcaggca tcgactacaa
     9841 cttcggcggt gcggtgctcg gtgcttcgct cggctacgat gattacgatg caggcttcaa
     9901 caataccggg gtcaactcga ccggcggcag cgcgcaggtg aagggaacca gcggttctct
     9961 ctatggcgcc tggtttggcc agaactggac gctcaacggc atcgccacct acggcaagct
    10021 gaccaccaat ctgtcgcgaa ccgtcaaata tacggtgacc tacaacacgg ccctggatcc
    10081 gcaggccgac atcaacgatc cgggctgcgc aggcgcagcc acctgcacgg tctcggtcaa
    10141 ccgtactctc gacggcaatc ccgatggccg ctctctggcc gtcggagcca ctgcgggcta
    10201 tcagacccaa gccgcctcct gggatgtcat gccctcactg agcgtcaact atcggcgcgc
    10261 ctcgttcgat tcgttcgccg agcacgatcc gaacgatccc aatgacggcc tgccgctgtt
    10321 cttcaatgat cagacggtcg agtccctgcg ctccatcctg ggcctggacc tgtccaagcc
    10381 ggtgagcact tcgttcgggg tggtcacgcc gattctgcgg gtcgagtggg atcatgagtt
    10441 caagacgagc gtccgcatga tcgatgcgca ctatgtgttc gacccgagct gcaagaacgg
    10501 agtctgcgac agcaacttcc tgctgccgac ggacccggcg gtcggcaact acggggtcgc
    10561 gggtgtgggc ctctcggtca ccctcgcgca gcgcgtgcag gcgtttgtgt acgatgagat
    10621 tctcttcggt tatcaggatt accacagcaa tgccgtgacg atcggcttgc gtgggcagtt
    10681 ctgataggca cgcatcaccg cccggggccg gtccgtacgt cggctccggg cacgccgcct
    10741 cagatcaaga ggcgcggaac cctcacctgc cctgctgact cttaaccgct aacggacgtg
    10801 gcgacaaagg cggggatctc tggacgtgcg cagccgagta gcaacgattg ccgcgctgct
    10861 tgtgccaatg gtgctcgcgg cggcctgcac cgcatcccgt gggacacggc accccaatcc
    10921 tcgccaggac atggagcgga ccatcgcctt tctgtcgaag agaagcgatc cggattcgct
    10981 ggcggcggca ggcgtgctgc ttctgcgcac ccaggcggat cagggcctgc tcctgcttgc
    11041 gcgggcgacc gcagctgctc cgagcagggt agacctggcc tggctgcaaa tccaggtctg
    11101 tctgcgaata ccgtcctgcg catcgcaacc gatggaggtc cacttgcgga cgctggatcc
    11161 gtccaacggc gccggctggc tgggtgcgtt gaaccgggcc gagtccgcgg gggatgcgag
    11221 cgcgaaggga gctgcactgc tgcacatcag ccatgccgat cgcgtcgcca tctactggac
    11281 gactctgatc agccgactga caccggcagt cgctcgggcc ggagtcctac cgttgccgca
    11341 ggcggaggtg gcggtcattg gcatgctcgc cgctgcggac attccgcctt ataagatcat
    11401 cgcggactat tgcatcagag ggcagctggc cgatgatcag gatgtggccc tgtgtcgcgg
    11461 catcggccga gcactggagc ggggtgacac ctatgccacc gagttgatcg gaatcgccat
    11521 tgcccggcat gtctggccgg aaaattcagc ggaatggaag gcagcggcat acgcccgcag
    11581 cgtgtacgaa tatcgaacta atttactggc acacctcgag gctcatgtgg ccctggaccg
    11641 gcacgcggcg gagggatacc tcgcgctctg cgcgaagtat ccgcgcgagc aggatgtcgc
    11701 gcgtgcgcgc ctgctcgaag ctggtgagaa gccggacccg cccgctcagt agcgcttgag
    11761 ctgcgcggcc ggcatccatc ccggcgactc gctcctctat atataggatg ctatcccggg
    11821 acgtcgcctt atgtcgcacg catgagtacg atcctgcatg cctcgagtag tcaggggtca
    11881 gttattcagc tgaggcattg caggtaagcg aaggagaaac catgccagat atcacaggaa
    11941 ttcgggtact gttcatcgcc ggttgcggtc ccatcgttcg cgacgggcct gcgagccgca
    12001 agctgtatgg ccaggcattg ggtatttcct tcaaggagga agcaggtgga tacctgcaca
    12061 ccgaggctct gagcggcgca aaaagtttcg ctctctggcc actcgccgag gcggctcggt
    12121 cctgttatgg cgaacccgcg tggcccggcg atgttcccgt tccgcaggcg tggattgaat
    12181 tcgacgtaga cgatatcgac aaggccacag cggagctgga atcacgtgga taccccatgc
    12241 tggttcgcaa caagaaagaa ccttggggcc agattgtcag tcgctttctg agcccggaag
    12301 gcttgctggt cgggatcacg ttcacaccac agatgcggga acagaaatag atcagtcagc
    12361 tcctgatgca gatgcgcctg agcggcgctc tctgagcgcg ctcacggttt ccgatcccgc
    12421 cgcgccgagg ggcaatgatc caggacatcc tcgaggtcgt atcgatacac gtcgcctgcc
    12481 tcatagtccg taagccagat cgcgtcgtgg ccgacgccga gcgcatctcc gcctggtcca
    12541 acccactggc acagaacgcg tcccgtgacc tcatcggtcg ctgaaagagg gaccttggcc
    12601 atggaagtcc acacgatgcc gtgggcaatc ttgatgtctc cgccatggcc gggtgtctgc
    12661 aatgagctgg agaaatccgc tcgtctggaa cgcgcgttga ttcgtgtcaa cgtgccatca
    12721 ccctgattca gggtccagac cgattggccg cccgcatcaa ggaatcgggg cctaggcccg
    12781 gtcggcacgg taccaatgac cgcgcccttg tcggcatcca cgactgttac ttctgcccca
    12841 gtaggtcgtg tgacccaaat ccgcccacca ctgaaagcca cattcagaga ccctcgagcc
    12901 agcgagatcc tctgtttgat gagaagcgag cttggatcga tcctcgcaag cgtcgcgcga
    12961 ccgtcgacga ccagccagac gctgtcgcgg ctcacggtga ttccgccttc ccgttccgca
    13021 ggtccagccg ccggcaccat gaccacctgg ctcgttagca aatccacccg agccagcgca
    13081 ttcggctcgc cgcaaagcgg cacccagagc ccaccgaatc cgaccgcgag ccctgcacac
    13141 ggctttcctc gaaggcagat cgctgcggtg cgcgtattgg agcgcggatc gatccgagcg
    13201 acgccatccg gaccggtagt gccaatccag acagtgtcgc cggtgacggc tacccaatcc
    13261 gccgtcttgc cgagatggat gcgcgctatg ggtgtcagct cggagatgag gtgtcgggtt
    13321 ggagcgggag cgtcttgcgc cgccgcgaag tgactgagtc tcgcggccag gagcaatgag
    13381 agcactacgg accgcgcgcc tctcaacaat gcactcagtg agccgttcag gattcccccc
    13441 cgccccggtt ggcccccctt gccaccagcg cttgtgctgt acgagccgtt cgcggtactt
    13501 tgcacaacaa agttacctag aatggccgtg aaagacgtgc gcatcgtatg ccggattttg
    13561 ctggattagt ccccgccccg accaatttct tcatgacgat ccgcgatgtg gcctactggc
    13621 tgcgatcggc ccgcgcggat gcgcgtctgg agcggtttcg gcagcaaggt ccgggcggct
    13681 cgccattcga tcatctgtac gccgagactg acgatccctt tggcgccgaa ttgccgcaat
    13741 accgttatca gcagcgcaaa tatgcctgcc tgctctcgat gctgccgcaa cgccgctatc
    13801 gaaacatact tgatgtcggc tgcggtctcg gcaccttcac gcgcaagctc gcaccgttcg
    13861 gtgagcaggt gctcggcacc gacatttcag gcgaggccat cagccaggcg agaagactgt
    13921 cgtttggtca ttcgaacatc acctatgcgc agcgggacat gctcgatgcc ccggctacgg
    13981 aatccggatt cgatctgatc atcattgccg acaccctcta ctacatcaat ccccggacgc
    14041 cggttgcatt gcaggccctc gcccaaacgt tcgcatcgaa gctgacgcca ggcggtctga
    14101 tcctgatcgt caatcacttt tttttcggcg tcgatgccgc ttccaggcag acccgtgaga
    14161 ttcatgacgt gttccgcggc gccccgagtc tcgagtgcat tgcggagatc cgccgcgcct
    14221 tctttttggc cacgcttctg caacgagcgg aagtatggag cgggtgatgg gaatcgaacc
    14281 cacgttagta gcttgggaag ctacagttct accattgaac tacacccgcg ccgacgaaaa
    14341 cccatcgcaa gggtaggcgc caccagaatc ttcaatggta catcatcggg agcgctttgc
    14401 gcgcagacgg ctggagcctg cggccgatac ctcgcctgca ccggcccgcg gattctcgct
    14461 caatgtaatc gtaccgggga tgtactgttt gccccagcgc acgaatgcca gcagcacttc
    14521 gcccagagcc ttgccttttg gggtgagcgc gtaggcgtag cgcacgggtc gctcctgata
    14581 gggagcgctg gtgatgatgc cggcgccttc caggcgctcg agacgatcgg caaggatgtt
    14641 ggtcggaatg cgctccggcg aatccgccaa ctcgccgtag gtctgcttgc catgcatcaa
    14701 atcacgcact acgagcagcg accacttgtc gcccacgagg tcgagcgagt tggcgatcgc
    14761 acaggctgag cgcgtgaagg gcttgggtgt tttcatccgg cttccgtttc gccatcctcg
    14821 aaggcagtaa cttgcagaat gcaagtcatt gtgctacggt ccctatatac ttgcaaaatg
    14881 caagtcactg aaggagtgtg tcatgacggc gcttgtcctc ccccagccca ccgagtctcc
    14941 cagcggtcag cgtccggttc gtctggccgt ttcgatagcg ctgcttgcct ggttcctgct
    15001 ggtcgtcgca ctgggtgctg tcggggcatt cgtcggacct cccgggacac cccctgtgcc
    15061 cattgcgttg ggcgttgcaa cgcccctggt catttttttt ctgctgctgc gtttctcgac
    15121 atcgttccgc gagttcgtgc tttccctcga tctgcgtctg gtcgcgggaa ttcaggcctg
    15181 gcgctgggcc ggcatgggat ttctcttcct ctatgcgtac aaggtgctcc cgggctggtt
    15241 cgcctttccg gctgggctcg gcgacatggc cattggcgcg accgccccgt ggatgattct
    15301 ttctctggcg cgtcagcctg gcttcgccgc gacgtccgca ttcgtccggt ggaatctgct
    15361 cggtatattg gatctcgccg tcgccgtgag catcggcgcc gtgagtgcca tctttgccac
    15421 gggaaacccc ggcgagctca gtgccgctcc aatggcgagc ctgccgttgt tactgattcc
    15481 ggcattcctc gtgccgtcat tcctcatgct ccacacaacg gcgctcatgc agagccgaca
    15541 actgcgccgg accaggatgt aagctaatct cagggcacgt gtagagataa tcagatccgt
    15601 tgagccgcgt agcgcccgcc tgccaggcgg gcccgacggc ccgtcaaaga gcggcgctaa
    15661 caatgcgccc gacctcacgc agcacgccat cgcgctcttg cggcgatccg ctgggtcgag
    15721 agtagaaagc cgtgatgagc acgggagctc gcccaggcgg ccacgcgatg aggacatcat
    15781 tgctctcgtc acggccgctg cctgtcttgt gcccgatacg ccactcgggc gggaggccgg
    15841 ccgggatact gtccttgccc accgtggcat tcaccatcca ggactcgagc tgttgccggg
    15901 acgagtcggc cagtgccttg cctagcaaca agctgtgcaa cagggagagc atggcagccg
    15961 gggtagtcgt atcgcggcca tcgccggctg ctacacggtt gagctcgggc tcgattcgat
    16021 cgagtcgcgt gatgcgatca cccagcgagc ggcagtactg agtgaagcct gcgggccctc
    16081 cgatgacccg gagcatcagg ttggctgcgg tgttgtcgct ccactgcacc gccgctgcgc
    16141 agacgtcgga aaggcgcatt ccgtgctcct gaacgtgctc gcgcatcaca ggcgcatact
    16201 cgagcagatc ctcgggtccg tagacaatcc agcgatcgag cttctcctcg ccacgctcca
    16261 cgcgttgcag caccgccgcg gcagccacga acttgaacgt gctgcacatc gcgaaccgct
    16321 cttcggcccg atagtggagc cttgcatggc tcgcggtatc caacatcgcc acgccgaggc
    16381 gcccgccgga gtgcttctcc agctcggtga tgcgctgctg gaggtctccg ggccccccgc
    16441 gggcctgcgt agccccctcg cctcgtgcgg ccgtagacag tgaacgtagt agagcgagcc
    16501 cgctaatcaa agccgctctt cgtgtcagga gcatgttcat ctgaaccttt gcgcccgtct
    16561 tgagccgaca tcctcgcatt tcaggtggct agaaagaaaa cgggagcctt tcggctcccg
    16621 tccacctcat aacgcaagct aggcgtcaga agcgatagtc gaccgcgagc agataactac
    16681 gcccgaggat accggggaag tacaaggagg ttgctccggc gaagttgcca cccgtgcccg
    16741 ctgcgagagc cgggaatggc ggctccttgt cgaacacgtt atcaacgacc agtcgagcct
    16801 ggaagttcgt cgtcacgttg aacgtgattg tcgaattgat caaccaccac gggttgaccc
    16861 gcagaatgtt ctgcgacgtc ggcgtgttca gattattgaa gttcatcccg ctgaagtact
    16921 gcgcctgcca gtaccaagac agcggaccct tggtccaggt caagctcgct gtgcccctgc
    16981 ccttaggcgt ggtggggaag cctcctgcat tgagctcgcc tgcctcgttc agcggggcag
    17041 ctgacccgac ttgcagcacg agccgacgcg tgtcgaggta ggcggcacgc gcttccaggc
    17101 tgccggcatt ccagggcaga tcgagcgtgt aatcgccact tgcggtaata ccctggaagt
    17161 gcagcaatcc ggcgttgatg aatcca
//
LOCUS       DASRHN010000177         8801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_57847, whole genome shotgun sequence.
ACCESSION   DASRHN010000177 DASRHN010000000
VERSION     DASRHN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57847"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="rpoN"
                     /locus_tag="VFB37_06855"
     CDS             <1..1141
                     /gene="rpoN"
                     /locus_tag="VFB37_06855"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZO22209.1"
                     /translation="WASDDEERQQEFADASGQSLQEHLLWQLELAKLEPREFAIARAI
                     IDSISDDGYLIDPLEEIANTLRPEVECDAAEVEAVLAGVQALDPPGIGARSVGECIEL
                     QLRQLDPATPGFNTAIQIAQRHLELVAERELSLLRRELRASEEEIACALALVRSCHPR
                     PGATVSTGAAEYVVPDVFVRRTDHGWSVEINAATLPRVRLNSSYASMIGRNASHATMR
                     AQLQEARWLLKSLEIRHETLIKVARSIVERQQAFLEQGDEHMRPMILKDIAEAVGMHE
                     STISRVTSGKYMHTPRGVFELRYFFSSQVEGADGSGTSSTAIRAKIRKLVKEEDTESP
                     LSDGRIADLLSGEGIPVARRTIAKYREAMGIAPSNERRRAGLKQM"
     gene            1193..1522
                     /gene="raiA"
                     /locus_tag="VFB37_06860"
     CDS             1193..1522
                     /gene="raiA"
                     /locus_tag="VFB37_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZO22210.1"
                     /translation="MHLNVTGHHIEVTAALRGYVEKKLERIARHFDQVIDVHCVLSVE
                     KLRQKAEATLHVSGNAIHADATEEDLYAAIDLLTDKLDRCVKKHKEKLTDHHAAEARR
                     TVKALMI"
     gene            1722..2624
                     /gene="rapZ"
                     /locus_tag="VFB37_06865"
     CDS             1722..2624
                     /gene="rapZ"
                     /locus_tag="VFB37_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZO22211.1"
                     /translation="MRIIILSGLSGSGKSVALHMLEDLGFYCIDNIPAALLKPFISYT
                     VRSPEPTYDRTAIGLDARNTAAEIATVPQLIDELRRSGIHCEVIFLVTSDEELLRRFA
                     ETRRKHPMSRSDVDLREAMALERQLLEPIVYAADLVIDTSKMGVHSLRDIIHQRVEQR
                     SAGRLSITFESFGFKHGIPGDADFVFDARSLPNPYWEPGLRNLTGRDPEVIRFLEAHT
                     NVAALIADIAQFMTARIPEYQSSNRGYLTVAVGCTGGQHRSVYIVDRLAQHFASVYPG
                     VTTRHGSLAGAKMFSPVKAARAGT"
     gene            2714..2842
                     /locus_tag="VFB37_06870"
     CDS             2714..2842
                     /locus_tag="VFB37_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22212.1"
                     /translation="MLFAVGIVSACAIAGLYLLARIVMLLGEINRKLGAIELDRQK"
     gene            complement(2852..4054)
                     /locus_tag="VFB37_06875"
     CDS             complement(2852..4054)
                     /locus_tag="VFB37_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZO22213.1"
                     /translation="MVEGAFQSYRSSARPTVGLVLTGGGSRSAYQIGVLLALAELMPR
                     ARNPFQVIVGTSAGAVAAGVLAAEAHQWRRAVVGLERVWANFRSEQVFHVDPVHMARA
                     GMHWVLALLSGGLILSPPKSMLDNSPLRQLLGFNVDVAGIRRSIARGHLRAIALCSTS
                     YSTGHSVAFYDAVEACHDWAREQRVGRRTILSLDHLMASAAIPLLFPAMKLGDEYFGD
                     GAMRQLNPLSPAIHLGADRLLIIGVRARRAAGVAPTQTRPMMPSPGEIFGYMLDTLFT
                     DQIYGDLEQLERINQVVLAAPQAAPVSRHVETLMLAPSVDPREVAARHSAELPRGLRA
                     LLRVIGGRDASGYQLASYLTFESGYTRELIALGYKDAMEARSPLMAFMSGEKMPKVVA
                     APGVVEVS"
     gene            complement(4170..6581)
                     /locus_tag="VFB37_06880"
     CDS             complement(4170..6581)
                     /locus_tag="VFB37_06880"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZO22214.1"
                     /translation="MRIRTLVIVVAAVILVPVAFVHGLLRASLPKLDGQLTHMGVVAP
                     VTIDRDERGIPTIEAANRVDLAFGTGFVHGQDRFFQMDLLRRLAAGELSELFGKAALE
                     QDEKARIFRFRNVARESLRLGTPEQRAVLEAYARGVNAGLRGLRSRPWEYWLLRSMPA
                     DWRPEDTVLVSLAMWWDLQYLDFDREIVRQEVNARLGGPQCESGWKCGLSFLYPAHTP
                     WDAPNSAGASAPAADSAPAAIPPPEILDVRGAANVARTQMRAFPAGLRGIGSNNWAVA
                     GRLTATGAALVANDMHLSQRVPPTWYRARLRITGSSTQPALDLNGVTLPGAPLLVAGS
                     NGHIAWGFTNSYGDWIHVQHVACSALSEHEMQTPEGPVPLSVRQQEIRVHGEAPVIFP
                     VQSGPGGVLYQARPQKRACWFVTWLAQQPAATNMNLMGFERASSVQQALALAPGIGIP
                     QQNLALGDAAGHIAWTIAGRIPVGTGIGRTNGSAPWTTAETHPRIVDPPAGRIWSANA
                     RATDDEAQESAIGGDVASVGAGYDLGARARQIRDDLLAMQGAATPVDMLRIQLDDRAV
                     FLTRWQTLLLSVLDADSLRDQPRRAEFRRLVSTWEARASVDSVGYRLVRAYRDRTEEG
                     VWEMVLAALGITRDEQTTVPDQFESALWRLVNEQPMHMLAASYRSWHDFLLAQVDATL
                     AELSETCRELAHCTWGARKPVRIRHPLSRSLPLLSRLLDMPTVQLPGDHDMPRVQDGP
                     VGASERFAVSPGHEDQGYLELPGGQSGHPLSAYYRSGFRDWAEGIRAPFLPGPARHRL
                     TLQAE"
     gene            complement(6605..8041)
                     /gene="pyk"
                     /locus_tag="VFB37_06885"
     CDS             complement(6605..8041)
                     /gene="pyk"
                     /locus_tag="VFB37_06885"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZO22215.1"
                     /translation="MRRTKIVATVGPATDDLGVLTDMMHAGLNVVRLNASHGTVADRH
                     RRLMMIREAAKRADKCVGVLLDLGGPKIRIECFRDGKVELQEGRAFVLDTALDSKAGT
                     VEGVGVAYKDLPKDVVAGDTLLLCDGQIVLDVDRVSGTRIETRVRAGGELSDRKGLNR
                     QGGGISAPAISEKDRDDIRFVAEEGIDYMAVSFARAAADIEQARTLVREAGGEARVVA
                     KIERHEAVANLPEIIESADAVMVARGDLGVEMGYAELTGLQKTIIHQTRARNRIVITA
                     TQMMESMIQSPVPTRAEVSDVANAVMDGTDAVMLSAESATGRYPVKAVQAMAQVIEGA
                     EKYQLTHSRTRHRVEGQFKGTEEAIAMAVMYTANHLKVRAIVALTETGATPLWMSRIR
                     SDIPIYAFTRHQATRRRVTLFRGVYPVIFDVTDPESTEVLYRELFARLLELQLVKAND
                     LVILTKGELSGVQGGTNSMLILKVVLAQ"
     gene            complement(8038..>8801)
                     /locus_tag="VFB37_06890"
     CDS             complement(8038..>8801)
                     /locus_tag="VFB37_06890"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZO22216.1"
                     /translation="AHRAEASTHGVARFAPVACAGPLLVGELEALETALHKPARPLVA
                     IVGGSKVSTKLTVLESLLEKVDKLIVGGGIANTFLAAQGLPVGKSLHEAEMLDVARRL
                     MSKAKERGTEIPMPTDVVVAKEFAATAHADVRGINEVKPDEMIMDIGPDTADRFAERV
                     RSAGTIIWNGPVGVFEFDQFGEGTRALAQAIARSKAFSLAGGGDTLAAIEKYAVEDGI
                     SYISTGGGAFLEFVEGKTLPAVAVLEERARESARI"
BASE COUNT         1574 a         2992 c         2795 g         1440 t
ORIGIN      
        1 ctgggcgagc gatgacgagg agcggcagca ggaattcgcc gatgcctccg ggcagtcgct
       61 gcaggagcat ctgctctggc agctcgagct cgcaaagctc gagccgcgcg agttcgccat
      121 tgcgcgcgcg atcatcgatt cgatcagtga cgacggctac ctgatcgatc cgctggagga
      181 gatcgccaac actctgcggc ccgaggtcga gtgcgatgcg gccgaggtgg aggcggtgct
      241 ggccggcgtc caggcgctcg atccgcccgg catcggggcc cgctcggtcg gagaatgcat
      301 cgagttgcag ctgcgccagc tcgaccccgc gaccccggga ttcaacaccg cgatccagat
      361 agcccagcgc catctggagc tcgtggccga gcgcgagctt tccctgttga gacgcgagct
      421 gcgcgccagc gaggaggaga tcgcctgcgc gctcgcgctc gtgcggtcat gccacccgcg
      481 gcccggcgcc acggtcagca ccggggcggc cgagtacgtc gttccggatg ttttcgtgcg
      541 tcgcaccgat cacggctggt cggtcgaaat caatgcagcc accctgcccc gcgtccggct
      601 gaacagcagc tacgcgagca tgatcggccg caatgccagc cacgccacca tgcgggcgca
      661 gctccaggaa gcgcgctggt tgctgaaaag cctggaaatc cgccacgaga ccctgatcaa
      721 ggtcgcccgc tccatcgtcg agcggcagca ggcctttctc gagcagggcg atgagcacat
      781 gcggccgatg atcctcaagg acatcgccga ggccgtcggc atgcacgagt ccacgatctc
      841 gcgcgtcacc tccggcaagt acatgcatac cccgcgcggg gtgttcgagc tgcgctattt
      901 cttctcgagc caggtcgagg gagcggacgg ctcaggcacg tcctcgacgg ccatacgcgc
      961 caagatccgc aagctcgtca aggaagagga cacggagagc cccttgagcg acgggcgtat
     1021 tgcggatctg ctctccgggg aaggcattcc ggtcgcgcgc cgcacgatcg ccaaataccg
     1081 cgaagcgatg ggcattgcgc cctccaacga gcgccggcgc gcaggattga agcagatgta
     1141 gagaaacaac ccgttgcgaa gcaagtgaac aagagccagt aaggagtcgg atatgcacct
     1201 caacgtcacc ggccatcaca tcgaggttac tgcggcttta cgaggatacg tggaaaaaaa
     1261 actggaacgc atcgcacgcc acttcgatca ggtcatcgac gttcactgtg tgctcagcgt
     1321 agagaagctg cgacaaaagg ccgaggcgac gctacacgtt tcaggcaatg cgatccacgc
     1381 ggacgcaacc gaggaagacc tgtacgccgc catcgatctg ctgacggaca agctcgatcg
     1441 atgcgtcaag aaacataagg agaagctcac cgaccaccac gcggcggagg cgcgcagaac
     1501 cgtcaaggca ctcatgatct agctcattgg aaaaatcgcc agcaggcggc gatttttcgg
     1561 acatcagctc cgctgccggg cgccaaatgc gcgaatttgg cctcctgcgc cataggggtg
     1621 agcagccctg agccgccttc cccccgcggc cgcatttccg attgaaggag ctgccattcc
     1681 gcccttgcgc accctcctgc cgcggggcta acgtcgcgcg cgtgcgcatc atcatcctca
     1741 gtgggctttc cggatccggc aagagcgtgg cgctgcacat gctcgaggac ctcgggtttt
     1801 actgcatcga taacattccg gcggcgctgc tcaagccgtt catctcctat accgtgcgca
     1861 gccccgagcc cacctacgac cgcacggcga tcggactgga tgcgcgcaac acggcggccg
     1921 agatcgcgac cgtgccgcaa ctcatcgacg agctgcgacg cagcggcatc cattgcgaag
     1981 tgatcttcct cgtcacgagc gacgaggagc tgctgcggcg cttcgccgaa acccggcgca
     2041 agcatcccat gagccgcagc gatgtcgatc tgcgcgaggc aatggcgctc gagcggcagc
     2101 tgctcgagcc gatcgtctac gccgcagacc tcgtgatcga cacctccaag atgggcgtgc
     2161 attcgctgcg cgatatcatt catcagcggg tcgagcagcg ctcagccggc cggctctcga
     2221 tcaccttcga gtccttcggc ttcaagcacg gcatccccgg agatgccgac ttcgtcttcg
     2281 atgcgcgctc gctccccaat ccctactggg agcccggcct gcgcaacctg accggccgcg
     2341 atccggaggt aatccgcttc ctcgaggctc acacgaacgt cgcggcgctg atcgccgaca
     2401 tcgcgcagtt catgactgcg cggatcccgg agtatcagag cagcaaccgc ggctacctga
     2461 ccgttgcagt aggctgcacc gggggtcagc accgttccgt ttacatcgtg gaccgcctcg
     2521 cacagcactt tgcgagcgtc tatccagggg tgaccacccg ccacggctcg ctggccgggg
     2581 cgaagatgtt cagccctgtg aaagctgccc gcgccgggac ctgagggcgc gagcgcagag
     2641 gcggcgctgc ggcgagcgcg cgccggctca cgcgtccatg cactgcagca acctgactcg
     2701 actggagaac tgaatgttat ttgccgtcgg catcgtttcg gcatgcgcga tcgcaggcct
     2761 gtatctgctg gcccgcatcg tcatgctgct tggggagatc aacaggaagc tcggcgccat
     2821 cgagctcgac cggcagaagt gatcccgcgg cttacgaaac ctccaccact cccggcgcgg
     2881 cgacgacttt cggcatcttc tcgccgctca tgaaggccat gagggggctg cgcgcctcca
     2941 tcgcgtcctt gtacccgaga gcgatcagct cgcgggtgta gcccgattcg aacgtgagat
     3001 agctcgccag ctggtagccg gaggcatcgc gcccgccgat cacacgcagc agggcgcgca
     3061 agccgcgcgg cagctcggcg gaatgtcggg cggcgacctc acgtggatcc acactcggag
     3121 ccagcatcaa agtctcgacg tggcgggaga ctggggccgc ctgaggggcc gcgagcacga
     3181 cctggttgat ccgctcgagc tgctcgagat ctccgtagat ctggtccgtg aacagcgtgt
     3241 cgagcatgta gccgaagatc tcacccgggc ttggcatcat cggacgcgtc tgggtcggtg
     3301 ccaccccagc ggcgcgccgg gcgcgcaccc cgatgatcaa caaccggtcc gccccgagat
     3361 ggatcgcggg actgagcgga ttgagctgcc gcatcgcgcc atcgccgaaa tactcatcgc
     3421 cgagtttcat ggccggaaac aacagcggga tggccgcgct cgccatgaga tggtcgaggc
     3481 tcagaatcgt gcgccggcct acgcgctgct ctcgcgccca gtcgtggcag gcctcgacgg
     3541 cgtcatagaa ggcgaccgaa tggcctgtgg agtaactggt cgagcaaagt gcaatggcgc
     3601 gcagatgacc gcgcgcaatg ctgcgacgga ttccggccac atcgacgttg aagccgagca
     3661 gctgccgcag gggggagttg tcgagcatcg acttgggcgg cgagaggatc aggccgccgg
     3721 agagcagcgc cagcacccag tgcatgccgg cgcgcgccat gtgcaccgga tcgacgtgga
     3781 aaacctgctc ggagcggaag ttggcccaca cccgctcgag ccccaccacg gcacggcgcc
     3841 actgatgcgc ttcggctgcg agcacgcccg cagcaacagc gcccgccgag gtgccgacaa
     3901 tcacttgaaa aggatttcgc gcccgcggca tcagctcggc aagcgccagc agcacgccga
     3961 tctggtaagc cgatcgcgag ccgccacccg tgagcaccag cccgacagtg gggcgggcgg
     4021 aggaccggta ggattggaac gcgccttcca ccatgactga ccggattatg aatcgccgcc
     4081 tcgcgcgctg gatgtgatgt agatcgcgcg cgccgtcctc aagctcaggc gcaagcccgc
     4141 taggctcagg ggccaccggg ccccgattct cattcggcct gcagcgtgag tcgatgccgg
     4201 gcgggacccg gcaggaaggg cgcccggatg ccctcggccc agtcccggaa tccgctgcgg
     4261 taatacgccg acagaggatg tccgctctgg ccgcccggca gctcgaggta accttgatcc
     4321 tcatgccccg gcgagaccgc aaagcgttcc gaagcgccca ccgggccgtc ctgcacgcgc
     4381 ggcatgtcgt ggtcgcccgg cagctgcacc gtgggcatat cgagcagtct ggagagaagg
     4441 ggcagcgagc gtgagagcgg atgacggatc cgcaccggct tgcgcgcccc ccacgtgcag
     4501 tgcgccagct cgcggcatgt ctcactcagc tcggcgagcg tcgcatcgac ctgggcgagg
     4561 aggaagtcat gccagctgcg atagctcgcg gccaacatgt gcatcggctg ctcattcacg
     4621 agccgccaca gggccgactc gaattgatcg ggcaccgtgg tctgctcatc tcgcgtgatg
     4681 ccgagcgccg ccaacaccat ctcccagact ccctcctcgg tacgatcccg gtacgcccgg
     4741 acgagccggt agccgaccga atcgacgctg gcgcgcgcct cccaggtgga caccaggcga
     4801 cggaactcgg cgcggcgcgg ttgatcccgg aggctgtctg catccaacac ggacagaagc
     4861 agcgtttgcc agcgggtgag gaacaccgca cgatcatcga gctgtatgcg cagcatgtcg
     4921 acgggcgtgg cggcgccctg cattgccagc agatcatcac ggatctggcg cgcgcgagcg
     4981 cccaggtcat acccggcccc cacggaggcc acatcgccgc cgatcgcgct ctcctgcgct
     5041 tcgtcgtccg tcgcgcgcgc attggcgctc cagatccggc ccgccggcgg atcgacgatg
     5101 cgtggatgcg tctcggcagt agtccagggt gcgctcccat tcgtgcgccc gatgccggtg
     5161 cccaccggga ttcttccggc tatcgtccag gcgatgtggc ccgcggcatc gccgagggcg
     5221 agattttgct gcgggatgcc gatgcccggt gccaaagcca atgcctgctg gaccgagctc
     5281 gcacgctcga agcccatcag attcatgttg gtggccgctg gttgctgcgc cagccaggtg
     5341 acgaaccagc aggcgcgctt ctgcggccgc gcctgataca gcaccccacc cggcccgctc
     5401 tgaaccggaa agatgaccgg cgcctcaccg tgcacccgga tctcttgctg gcgcacgctg
     5461 agcgggaccg gaccttccgg ggtctgcatc tcatgctcgc tcagggcgct gcaggccaca
     5521 tgttggacat ggatccagtc gccgtaactg ttggtgaatc cccaggcgat atgcccgttc
     5581 gacccggcaa ccagaagagg cgcaccggga agtgtgacgc cattgaggtc cagcgcgggc
     5641 tgggtcgatg aaccggtaat gcgcaatctc gcacggtacc aggtgggtgg cacccgctga
     5701 ctcaagtgca tgtcgttggc cacgagcgcg gcgcccgtgg cggtcagccg cccggccacc
     5761 gcccagttgt tgctcccaat tccgcgtaag ccggcgggaa aggcccgcat ctgcgtgcgc
     5821 gcgacattgg cggccccgcg cacatcgaga atttcgggcg gcggaatggc ggcgggagcg
     5881 gaatcggccg ccggagccga agcgcccgcc gagttgggcg catcccacgg ggtgtgcgcg
     5941 ggatacagga agctgagacc gcacttccag cccgactcgc actgcgggcc gcccagccgc
     6001 gcgttgacct cctggcggac gatctcccga tcgaagtcga ggtattgcag gtcccaccac
     6061 atggcgaggc tcacgagcac agtgtcctcg ggcctccagt cggcgggcat cgagcgcagc
     6121 agccagtatt cccagggccg gctgcgcaaa cctctcagcc ccgcgttgac cccgcgcgca
     6181 tacgcttcca gcacggcgcg ctgctcgggc gtgcccaggc gcagcgactc gcgggccacg
     6241 ttgcggaacc ggaagatgcg ggctttttca tcctgctcga gcgcagcctt gccgaacagc
     6301 tcggacagct cgccggcggc cagccgccgc agcagatcca tctggaagaa gcggtcctgg
     6361 ccatgcacga agcccgtccc gaacgcgaga tcgacccggt tcgccgcctc gatggtgggt
     6421 atgccgcgct catcccgatc gatcgtgacc ggagcgacga cccccatgtg cgtgagttgc
     6481 ccgtcaagct tcggcagcga cgcacgcaac agcccatgta cgaatgcgac cggcacgaga
     6541 atcacggctg cgaccacaat caccagggta cggattctca tgcgccgtgc cgctggctcg
     6601 ctcgtcactg agcaagaacg accttgagaa tcagcatcga gttggttccg ccctgcactc
     6661 ccgacagctc gcctttggtg aggatgacca gatcgttggc cttcacgagc tgcagctcca
     6721 gcaggcgcgc gaacaactca cggtagagca cttccgtgga ttcggggtcc gtcacatcga
     6781 agatgaccgg atagaccccg cggaagagag tgacccggcg tcgtgtcgcc tgatgacgcg
     6841 tgaaggcgta aatcggaata tccgagcgga tgcgcgacat ccacagcggt gtcgcgcccg
     6901 tctcggtcaa cgcgacgatt gcccggacct tcaagtggtt tgcggtgtac atcaccgcca
     6961 tggcaatcgc ttcctcggtc cctttgaact gaccctcgac ccggtgccgg gtgcgcgaat
     7021 gcgtgagttg atatttctcc gcgccctcga tgacctgcgc catggcttgg accgccttga
     7081 cggggtagcg gccggtggcg gattccgcag acagcatcac cgcatcggtg ccgtccatga
     7141 cggcattggc gacatcgctg acttccgcgc gcgtcggtac cggactttgg atcatcgact
     7201 ccatcatctg agtcgccgtg atgacgatgc gatttcgggc tcgggtctgg tgaatgatcg
     7261 tcttctgcag accggtcagc tcggcatagc ccatctcaac gcccaggtca ccgcgcgcca
     7321 ccatcaccgc atccgccgat tcgatgatct cgggcagatt ggcgaccgct tcgtggcgct
     7381 cgatcttggc gacgacgcgt gcttccccac ccgcttcgcg caccagtgtc cgcgcctgtt
     7441 cgatgtcggc agcggcccgg gcaaaggaga ccgccatgta gtcgatgccc tcttcggcga
     7501 cgaagcggat gtcgtcccga tccttctccg agatcgccgg cgccgagatg cccccaccct
     7561 ggcggttgag ccctttgcgg tccgacagct ctccgcctgc acgcacgcgg gtctcgatgc
     7621 gtgtaccgct gacccgatcc acgtccagga cgatctggcc gtcgcacagc agcaacgtgt
     7681 ctcctgcgac cacgtccttc ggcagatcct tgtaggccac gccgacacct tcgacggtgc
     7741 ccgccttgga atccagcgcc gtgtcgagca cgaaggcccg gccttcctgc agctcgacct
     7801 tgccgtcgcg gaaacactcg atgcggatct tcggtccgcc gagatcgagc agcacgccca
     7861 cacacttgtc ggcgcgcttc gccgcttcgc ggatcatcat caggcggcgg tgccgatccg
     7921 ccaccgttcc gtgggaggcg ttcagccgga cgacgttcag accggcatgc atcatgtcag
     7981 tgagaacacc cagatcgtcg gttgccggcc cgacggtcgc cacaattttg gttcgtctca
     8041 tatccttgct gattcccgcg cacgctcctc cagaaccgcc accgcgggca gcgtcttccc
     8101 ttcgacgaac tcgaggaagg ccccaccgcc ggtagagata taggaaatgc catcctcgac
     8161 tgcgtacttc tcgatcgccg cgagcgtatc gccgccgcct gcgagggaga aggctttgct
     8221 gcgggcaatc gcctgcgcca gcgcgcgcgt gccctcgccg aactgatcga attcgaacac
     8281 gcccacgggc ccgttccaga tgatggtgcc cgccgagcgc acccgctcgg caaaacggtc
     8341 ggcggtgtcc ggtccgatgt ccataatcat ctcatccggc ttcacttcgt tgatgccgcg
     8401 cacgtcggcg tgcgccgtgg ccgcgaattc cttggccacg accacatccg tcggcatcgg
     8461 aatctcggtg ccgcgctcct tggccttgct catcaggcgg cgtgcgacat cgagcatctc
     8521 cgcctcgtga agtgacttgc cgacgggcag gccctgtgcg gccaggaacg tgttggcaat
     8581 acccccgccg acgatcagct tgtccacctt ctccagcagc gactcgagca ccgtcagctt
     8641 ggtggagacc ttggagccgc cgacgattgc gaccaggggc cgcgccggtt tgtgcagagc
     8701 ggtctccaga gcctcgagct cgcccacgag cagcgggccg gcgcaggcaa cgggcgcgaa
     8761 gcgtgcgacc ccgtgcgtac tggcttcagc gcgatgagca g
//
LOCUS       DASRHN010000178        19913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2201167, whole genome shotgun sequence.
ACCESSION   DASRHN010000178 DASRHN010000000
VERSION     DASRHN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19913
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201167"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1770)
                     /locus_tag="VFB37_06895"
     CDS             complement(64..1770)
                     /locus_tag="VFB37_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZO22217.1"
                     /translation="MKKTLSPAPPPGTGKKRKRRALRGRMVDPQALAQVEALVPEPRR
                     RDLLIEYLHRINDRYGQLGAPHLAALAKLLRLAQTEVYEVATFYHHFDVVKEGPGGAV
                     AAPPALTVRVCDGLSCEMAGAKALLERLPQLLGASVRVIPAPCVGRCEQAPVAVVHQK
                     PIPQATPELVHSQVEAGHLHDKPAPYIGFDAYGKAGGYVLLRDCLEGRREVEKVLKTM
                     EDSGLRGLGGAGFPAGRKWRIVRNERAPRLMAVNLDEGEPGTFKDRWYLERDPHRFLE
                     GMLIAAWAVDVSAIYIYLRDEYHGCRAILEAELARLEAAPPVLNLPKIELRRGAGAYI
                     CGEESAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNFETLYWVRDILEKGADWFA
                     SQGRNGRKGLRSFSVSGRVRDPGVKLAPAGITVRELIDEYCGGMQEGHSFYGYLPGGA
                     SGGILPASKGDIPLDFDTLQPHGCFIGSAAVIILSDHDTAKAAARNMMRFFEHESCGQ
                     CTPCRVGTAKANILMAEERWDQSQLEDLSQVMRDASICGLGQAAPNPLDCVLKYFPNE
                     LT"
     gene            complement(2119..2853)
                     /locus_tag="VFB37_06900"
     CDS             complement(2119..2853)
                     /locus_tag="VFB37_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZO22218.1"
                     /translation="MTALIFVAFLWAGAQNALAGGGSFITLPVLMLTGLDARTANITS
                     TLALFPSQVAAGWTGRRSVADAAGLSFRALALISIAGGALGAALLLVTSQSFFAHLVP
                     WLVLFATVVFAWSSFGARPEQARRLGRSGAALTQLCISVYGGYFGGAIGFLMFATLAA
                     AAMPLRAAGATKNVLAVAMNASAVAIFAFSPQARWGPAVLGAAGAIIGGVVGGLLLQR
                     INERLLRGLVIVIGVALSIGLFVRAH"
     gene            3049..3858
                     /locus_tag="VFB37_06905"
     CDS             3049..3858
                     /locus_tag="VFB37_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO22219.1"
                     /translation="MLKTFRTLVLVLALGIAGPVLAQADPTLHQIYEAAQAGHLAQAQ
                     QMVDQVLRDHPQSAKAHYVAAELHAREGNLPSARQELNTAQRLEPGLPFAKPDSVREL
                     QRELGQGQVLRTSPAFGVPGYARAHSSVPWGWILVLVAAIAVLAAMFRRRASPTVYTQ
                     YPGGTAAPVGPPGYGPGYAGPGYGPPGVGSGIAGGLASGLAVGAGVVAGEEIAHHFLD
                     GNRVEGNPYPVNDPQADNPDMGGSDFGVSDGGSWDDGGSVGGDVSGGDDWT"
     gene            complement(3876..5426)
                     /locus_tag="VFB37_06910"
     CDS             complement(3876..5426)
                     /locus_tag="VFB37_06910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZO22220.1"
                     /translation="MASKSYKEFSASHREAPGPSCRLVGEVPAEERVEISIYLKPRDG
                     APAASAPGTDARAAQRARRASQHAGDIELLRSFARDHGLTVLSAEPGRRLVRLAGTAS
                     QAQAAFQTKLSRYTDGKRTFRARTGSLSLPAELLPVVESVLGLDTRPAAQPRLVSRPR
                     AAAAAAFLPNQVTALYGFPTSVTGTGQCIALIELGGGYTNSDTSAAFKAMGLAPPSVV
                     AVPVDGGANTPSPDDGADDEVALDIQVAGGAAPGAKIAVYFAPNTDAGFLDAITSAVH
                     DATNKPGVLSISWGSAESTWTQQSMQSMNSALQDASTLAVSVCIAAGDSLATDGVTDG
                     KAHVDFPASSPWAIGCGGTTITVSGNSISSEVVWNDGDSGTGGGISDVFPVPSFQQSA
                     SLPPSVNDGKTRRGVPDVAGDAAPDSGYRILVGGKPGVVGGTSAVAPLWAGLIALINQ
                     GAGHTIGFCLPFLYAHATLLRPITRGDNTATGTSLGYEARPGWNACTGLGVPRGQQLF
                     DALTAGGS"
     gene            complement(5526..7322)
                     /locus_tag="VFB37_06915"
     CDS             complement(5526..7322)
                     /locus_tag="VFB37_06915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO22221.1"
                     /translation="MRRWPLLIIVALAPLWLIGILDRGAWTPDEPREADIAWRMSLQH
                     DWTLPHLAETPFLEKPPLSYWMSALALHIYGDSPAAARLPDILYAFLTALAIAVLAFA
                     MEGPSTAVVAAIAAGTAITAYRVAIWLAPDACLLAGCAIALLGAYLGFTAEPGRRKLL
                     GYTILHAGAAVGFMAKSAPGWLVPALALGTIIVWERRWSELLRWELYAGLLLQALIIL
                     PWVYAVSQTEHGMQSLSALFWHNTFGRFTRISGSAALDYTSGHHNSPGKYFRELPVYL
                     LPWTLLAVAAVRRAWNRVRVRGIQGTAWRFAIATIVPFLVLLSVAATARDIYAAPALL
                     GFGLLIGLWATEISVGHLAQRPTRLDLFALAGTRAIVGLIAAAAVALLGLLSVASGNL
                     TYLTTAAAMAALAIMALKFAAFMQGRDDLPRSLAWTYSAYAATLTLGGLAMFPTIDRW
                     QDLPALAREIREDSRGAPLALLNPDETTIAMIDHRPLSIVDRGRGPEAASSLPVPVPF
                     SVLDSSTQSPEIVVASWFRERGSRAHVLVKLPGRGPGELSRLLDRIMPSEPPGDGVAG
                     TLSSEHVVSIIHRYELPHGRRYALLGPHEQAR"
     gene            complement(7363..8901)
                     /gene="glpD"
                     /locus_tag="VFB37_06920"
     CDS             complement(7363..8901)
                     /gene="glpD"
                     /locus_tag="VFB37_06920"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HZO22222.1"
                     /translation="MSTLRERYDLLVVGGGINGTGIARDAAGRGLSVLLCEQHDLASH
                     TSSASTKLIHGGLRYLEDFHFGLVRKALQEREVLLGAAPHLMRPLRFVMPHDAHLRPA
                     WMIRAGLFLYDHLARRRLLAASAAVNLRTHVAGEPLLPGFQRGFIYSDGWTDDARLVV
                     HNAIDAAHRGATILTRTRCERLDLEGDHWLATVATAQQTLTVRARAVVNAAGPWVNEL
                     VRDRTPVRASRTVRLVKGSHIVVPQLFTHRFAYIFQNADRRIVFAIPYENEFTLIGTT
                     DVDYRGPLGTVSIDAQEIAYLCAVVNRYFRHPSSPADVVWSYSGVRPLLGDESADPSS
                     VTRDYALELDRIPAPLLSVFGGKITTYRKLAESAVDRLAHVLGCSRGPWTHAAKLPGG
                     DLPDGSLAAFLRTVERRYPWLPAGVRRRYAHAYGTRIERVVGNASMLADMGEELMPGL
                     YEREVDYLVNEEFASTAEDILWRRTKVGLHARGVDAGRLIARLTSSSTPAVQRPSALS
                     PDQA"
     gene            complement(8898..9824)
                     /locus_tag="VFB37_06925"
     CDS             complement(8898..9824)
                     /locus_tag="VFB37_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22223.1"
                     /translation="MPIRLFAPRYWPTWIGLGLLWLIALLPYRRLVQLGRPIGTVLRH
                     LPLSFVNTARRNIELCLPELSPAEREKLLVRHFESLGIGLFEIAFTWWSSLPRWQPLI
                     HIDGREHLEAALARGKGAILLTAHFTTLEVGGRILLSVAPVSFLYRPTRNEVLAHFLK
                     HRRCKYGGQPIPLNDIRTLISALKNNECIWYAPDQSYRKKGAQMVELFGIPAATNTFT
                     SRLARLTGAPVLPYFFERLPDGKGYHARIYPPFDDFPSESAVADTERFNHLIEAQVRR
                     VPEQYLWIHRRFKGLTADYPDYYNQARASRSV"
     gene            9887..11407
                     /locus_tag="VFB37_06930"
     CDS             9887..11407
                     /locus_tag="VFB37_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO22224.1"
                     /translation="MRRSSPADARYVEVSLRHLGLRRGDRAILGDVSWSIRPGQRWVL
                     VGPNGAGKTQLLKVVSGAVWPVPGKPSRRYRWRHEAFLSPLGVMDEIGYIGPERQDKY
                     ERYGWNHTVTEIVGTGLYRTDIPLDPLTARDRRQIAALLHRMGIEHLGPRRFLSLSYG
                     ERRLALLSRVLAARPKLLLLDELLSGLDAQNRSRALGWLERTGRARLPWVLTAHRLED
                     VPRSATHALVLNAGRIKYRGSARGAPLKRWLVQGKADRAQLGGARRSVDRLLLRLTRA
                     SVYVDEHRVLEALSFTLRSGECWVVHGANGSGKSTLLRTLYGDHRVAAGGRIERSGVA
                     AGVPLELFRRRVGLVAPHLQSGHPHQLTVSEVVQSGPHASIGLGAAARCPDRRAASHA
                     LELFGLQRLAARALHELSYGQLRRVLFARAWACRPALLLLDEPYAGVDARTRRDLMHL
                     VEGMLASNTAVVMATHHRREWPTFTTHEIELSAGRALYCGPVRSPLESRTRDGADP"
     gene            11404..12621
                     /locus_tag="VFB37_06935"
     CDS             11404..12621
                     /locus_tag="VFB37_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZO22225.1"
                     /translation="MRIVVGWAVLVLGVLAVLGIAHEPLFWVHYALTRIHGTHLPLFY
                     YEPRELVPGGNEPPPPRESPASESLDIGALEAAAAYAGAHHSRALIISRHGYLIYERY
                     WHGSRFDTLIESGPLGRVLAALVTGNAIYGRRIGWPDAPIGMFLSEWQDDPRGSITVR
                     NLLQSSSGLAVPPPAANPWSAAADQAYGSHVIAGYLQRSLQSPPGQKWVQQSVDPDLL
                     VLVIQKATHERYAQYLSRTLWRRIGAADAWLWLDRPEGDVHVDRGFLARQGDWLRVAE
                     LLLQNGRYQGSEVIDPRWVPQLLRPAKANADYGAYIRLGAHTLPGMTAYDAPDLFLVQ
                     GGGSYLWLIPSLQLAVLRTGEAPPAEQAGRTASDDSAWDEARIPNLVLHGTRDFVPAA
                     RSGAGISNIVPNH"
     gene            12672..13865
                     /locus_tag="VFB37_06940"
     CDS             12672..13865
                     /locus_tag="VFB37_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO22226.1"
                     /translation="MRKQLGSLFIVTVVDILGFGILIPLIPYMGMKFNTPPEWITPVM
                     GSYSLCQLIAAPLWGRLSDRYGRRPILMSSLAGACLSYLLLGFATNIWWLLLSRAING
                     FMAGNISAAFAYASDVSEPRRRAGALGLIGAAIGIGFTLGPAIGGILAGDDLRTASFL
                     RPAIVSACLSSCALLLVACVLPESHTERHRAEHPLGARIGPIRLLLQRPVLRLVAAAA
                     LLVTSAQSILDSILGIWALNRYGYGPRTFGVLMFGIAFFAILTQGVLVRYLAPRLGES
                     KLATLGALTFAAGLLIVALTSVPEMTVVGLALCGIGVGAFSPSASALASRQAASHDRG
                     AVMGTYQSASSLARVIGPFTSGPVYAAWGTSAPFLAGACAVIPAVLLIWRTRRIDAQR
                     TAATR"
     gene            complement(13855..14202)
                     /locus_tag="VFB37_06945"
     CDS             complement(13855..14202)
                     /locus_tag="VFB37_06945"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZO22227.1"
                     /translation="MTELRFESVLPLTGIRRGSMCAYTVAAREILICHTREGVFALDN
                     ICTHAHARMCEGWLKATRIVCPLHGASFDIRDGKVLGPPAELPLPSFPVRIVARMIEV
                     AVSPASAGASISE"
     gene            complement(14199..15104)
                     /locus_tag="VFB37_06950"
     CDS             complement(14199..15104)
                     /locus_tag="VFB37_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HZO22228.1"
                     /translation="MTTRKVQVHQVDAFTRERFTGNPAGVVLNADGLTEAQMLAIARE
                     LNNADTAFILNSDGDDHTLRARFFTPRTEVGFVGHASVAAHYVLSRRHNAPRRQRQKS
                     KAGIVDIEVRGQEEDRSIAIRQNTPPIGRELNDRERLAVLDSLALATDDLDPRCPLRI
                     VGSSSTRLLIGVRSADVLKHLKPDVNRLTTLSAQVGAAGYFVFALSSGSGAHLTESRM
                     FCPALGITEDPVSGNAHGLLGIYLARTGLLAREGTRVSFTGIQGHQLHRPGRVNVELE
                     LKDGSIEGVWIIGQAVSIFETEIAL"
     regulatory      15269..15418
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15411..17528
                     /locus_tag="VFB37_06955"
     CDS             15411..17528
                     /locus_tag="VFB37_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22229.1"
                     /translation="MRSFPSAVFLLAPCALALASPESEPPLQEVVITGTLRPTSEQDV
                     PASASVLDSATLEQAGEQHLEDVIGLVPNLNWAGDTSRPRYFQIRGIGELDQYQGAPN
                     PSVGFLIDDIDFSGLGTAATLFDIEQVDVLRGPQGTRYGANALGGLIYVQSAAPQFTY
                     GGRVELGAGDFGMRSYGAVLTGPVDELDSAFRIAAQRYTSDGFYHNAYLHRDTDDNDE
                     LTLRARWRWRPSDQLRVDLTVLDVHIDNGYDAFSIDNSRTTQSDQPGEDVQHSTGVSL
                     RADYTGLGPMTLTLIGTLANTGIRYGFDSDWGNPISWAPYTYEFTDNQRRDRHTHSLE
                     LRLASTGEHALDWLFGLYALDLSEGFNDTSPGLYLDPFDPTQDAHTLAITTSHYHSRN
                     EAVFGELDGALTRRWHWTAGLRAERRIADYHDLTTNLGEPDATHAFSPVDHMWGGDLS
                     LQYRLAAEQRLYVQLARGYKAGGFNLGPGLPTDQLLFNPESDVNLELGYKADLAEHRV
                     RIDADVFYTHRTGLQLQTGEQLVPQDPTTFILYTANAASGHNYGLESSLSWQPISLLE
                     LLASAGLLNTAYHGLTLDGERLPDRALPHAPSWQAALSSILTLRQGYFLRLDLSGKGS
                     FYYDVPALDPYASSAYMLLNAKAGVQRESWGANIWMRNVTDRRYTVRGFYFGDVPPDF
                     PSQQYVQLGEPRSFGAQITYSFH"
     gene            17553..17795
                     /locus_tag="VFB37_06960"
     CDS             17553..17795
                     /locus_tag="VFB37_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HZO22230.1"
                     /translation="MEIGVEISLYPLNQHFIAPIQDFIDRLNADGRFKVVTNSLSTQV
                     FGRYEEIFDSLTRELRITFENNDKAVFVMKVIGPMT"
     gene            17792..18415
                     /gene="pnuC"
                     /locus_tag="VFB37_06965"
     CDS             17792..18415
                     /gene="pnuC"
                     /locus_tag="VFB37_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HZO22231.1"
                     /translation="MIDWRHLLTSLVAGLEATTAPEAAAVVLGLTYSLLAIRRSRWCW
                     VAGGASSAILIGLSWARHLPMQAGLNIYYVAVSVYGWWHWSREQAEQGSPQVSRWPLY
                     KHLLACLGVVLASALTARWLSAETQAAWPFLDPLTTWGSLLATWLAARVKLENWLYWL
                     VIDSLLAFLFGSQGLHFVALLSIVYLGFSTVGFLRWRKSYRALACAN"
     gene            18403..19272
                     /locus_tag="VFB37_06970"
     CDS             18403..19272
                     /locus_tag="VFB37_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZO22232.1"
                     /translation="MRELMHEAGLEAALAHVPGYVPGRCEGTVRRLSGGSVNRSYRVC
                     TPAGGYVVRLSPVTDAWLAADRSVERELHARAAAAGIAPRIVHADGNDRWLITELVEG
                     RLWSEADFGKPHSLGLLADTLSVVHALEPPAGAPIDLLGTIGAYARQLQPADPRLEGW
                     LDAAARAWPLSGAPERQPAIVHHDLHASNLIETGAGLRLIDWECAAVADPLLDVACIL
                     SYYETAWSCAALLLERAGLRGIDASQLRASVWLFDLHTWLWYCERRQRLSPTQAELDA
                     EQRLSARLMRGVA"
     gene            19394..19708
                     /locus_tag="VFB37_06975"
     CDS             19394..19708
                     /locus_tag="VFB37_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZO22233.1"
                     /translation="MAVLRVVDRDGVEHEVDAKTGLKVMETLRELDYGVAAICGGMCS
                     CATCHVYVDPQWLAKLPAPMSDERELLSELSHHEVNSRLSCQIEFTQALAGLRVTIAP
                     DE"
     gene            19718..>19913
                     /locus_tag="VFB37_06980"
     CDS             19718..>19913
                     /locus_tag="VFB37_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO22234.1"
                     /translation="MSTSSVQVETRGAVALVTLNRPDNANTLNLQTAMDLLAAAMTCD
                     RNPAVRAVVLRGAGRHFCFGG"
BASE COUNT         3361 a         6451 c         6666 g         3435 t
ORIGIN      
        1 gtaatcgtgg gcaccgcgct ctgcgctcgc tccatgcggg taatggcgtt catcggtgct
       61 ccttcaggtc aactcgttcg gaaagtactt cagcacacag tccagtgggt tcggcgccgc
      121 ctgccccagg ccgcagatcg aggcatcgcg catcacctgc gacagatcct ccagctgcga
      181 ctgatcccag cgctcctcgg ccatcaggat gttggctttg gcggttccta cccggcacgg
      241 cgtgcattga ccgcaggatt catgctcgaa gaaacgcatc atattgcgag cggccgcctt
      301 ggcagtgtca tggtcggaca ggatgatcac ggcggccgag cctatgaaac aaccatgggg
      361 ctgcagcgtg tcgaaatcca gcggaatgtc tcccttcgag gccggcagga tgccgcccga
      421 ggctcccccg gggaggtagc cgtagaagct gtggccctcc tgcatgccgc cacagtattc
      481 gtcgatcagc tcgcgcaccg tgatcccggc cggcgcaagc ttcacacccg gatcccgcac
      541 ccggcccgac accgagaagc tgcgcagacc cttgcggccg ttgcgcccct ggctggcaaa
      601 ccagtcggcg cccttttcca gaatgtcccg aacccaatag agcgtctcga agttgtgctc
      661 gagcgtcggt cgtccaaaga ggccgacctg ggcaacatac ggcggacgca accgcggcat
      721 gccgcgcttg ccctcgatcg actcgatcat cgccgactct tcgccgcaga tgtacgcccc
      781 ggccccgcgc cgcagctcga tcttcggcag attcaatacc ggcggtgcgg cctcgagccg
      841 tgcgagctcc gcctccagga tggcgcggca gccgtgatac tcatcgcgca ggtagatata
      901 gattgcagac acatcgaccg cccaggcggc aatcagcatg ccttcgagga atcggtgcgg
      961 atcgcgctcc agataccagc gatccttgaa cgtgccgggc tccccctcgt cgaggttgac
     1021 ggccatcaat cgtggggcgc gctcattgcg gacgatgcgc catttgcgac ccgccggaaa
     1081 tccggcgcct cccagtccac gcagtcccga atcctccatg gtcttgagaa ccttctcgac
     1141 ctcgcgcctg ccttccaggc agtcccgcag cagcacgtag ccgcccgctt tcccgtaggc
     1201 gtcgaagccg atgtaagggg caggcttgtc gtgaagatgt ccagcctcga cctgcgagtg
     1261 caccaattcg ggcgtcgctt gcgggatggg cttctgatgc acgacggcga cgggggcctg
     1321 ctcgcaacga ccgacacagg gagcgggaat gacgcgtacc gaggctccga gcagctgggg
     1381 cagccgctcg aggagtgcct tcgcgcctgc catctcgcag ctcaggccat cacacacgcg
     1441 caccgtcagg gcgggcggtg ctgcgacggc tcctccggga ccctccttca ccacgtcaaa
     1501 atgatgatag aaggttgcca cttcatagac ctccgtctgc gccagacgca gcagcttcgc
     1561 aagcgctgcc agatggggcg ccccgagctg gccgtatcgg tcattgatcc ggtgcagata
     1621 ttcgatgagc aggtcgcgcc gacgcggctc cggcaccagg gcctccacct gtgcaagggc
     1681 ctgcgggtcg accatgcgcc cccgtagtgc ccggcgcttg cgcttcttgc cggtgccggg
     1741 cgggggtgcg ggactgaggg tctttttcat aggtttcagt atggtggggc gctcgcgcgg
     1801 gtgtccaatc cattatggag atccggccat ccacgcgcgt tatggaactc gcggttggaa
     1861 accggcgcgt tgcaggatac ggaaggctgg gcgggttgcc catgccgggc cgcgagcggc
     1921 gccgtgcacg gttgggatgc gcgcgaactc agaatttgaa cttcgctggg ctgcggaagt
     1981 tcccatcgcc agagcccggg aattgcgagg ggcagagctc gcaagttgcc ggtgctggcg
     2041 aatcggcaag ctccggctgt gggcgaaagc cgatgccgga cgtgcgacat cacacttctt
     2101 aaggtcaggg ttcgagccct agtgagcgcg cacgaacaat ccgatactca gtgcaacccc
     2161 gatcacaatg acgagcccac gcagcagtct ctcgttgatc cgctgcagca gcagtccgcc
     2221 cacgactccg ccaatgattg cacccgccgc gccgaggacg gcggggcccc agcgcgcctg
     2281 cggtgagaag gcgaaaatcg ccacggccga ggcgttcatc gcgacggcca gcacattctt
     2341 ggtcgcgccg gcggcgcgca ggggcatggc ggccgcggcc agggttgcaa acatcagaaa
     2401 gccaatggcg cccccgaaat aaccgccata gacagagatg catagctgcg tgagtgcggc
     2461 gccgctgcgg ccgagccggc gcgcctgctc cgggcgagcg ccgaaactgc tccatgcaaa
     2521 gacaaccgtg gcgaacaaca ccagccaggg cacgaggtgc gcaaagaacg attgtgaagt
     2581 gaccagcagc agggcggccc ccagcgcacc gccggcgata ctgatcagcg cgagcgcgcg
     2641 aaaggacagg ccggctgcat ccgccaccga gcggcggccc gtccagccgg cggcgacctg
     2701 tgagggaaac agcgccaggg tcgaagtgat gttcgccgtg cgcgcatcca gtccagtcag
     2761 catcagcacc ggcagggtga tgaacgagcc gccgccggcg agcgcgttct gcgcacccgc
     2821 ccagagaaac gctacgaaaa tgagcgctgt cacggaggct cccgccagtg ccgcctcgcg
     2881 gggaggctag cccctggctt gagcagggca gtgacgagtc gagcgggaac ccgccccgaa
     2941 gcttgctgcc caaagagagg atcgatcggt gcggggctcc cttgagctgg cgcgtaccga
     3001 ccgcatattc gggacgggtc ccagacccta ttcatcaaga ggcaggccat gctcaaaact
     3061 ttccgcacgc tggtactggt gctcgccctc ggaattgccg ggcccgtact cgcccaggcc
     3121 gacccgacgc tacaccagat ctacgaggct gcgcaggcag gccatctggc ccaggcacag
     3181 cagatggtgg atcaggtgct gcgggatcat ccgcagagcg cgaaggcgca ctacgtggcc
     3241 gccgagctcc atgcgcggga gggtaacctt ccctcggcgc ggcaggagct caatacggcg
     3301 cagcggctgg agcccggatt accctttgcc aagccggatt ccgttcgcga gttgcaaagg
     3361 gagctcggac aggggcaggt gttgcgcacc agcccggcct ttggggtgcc cggctatgcg
     3421 cgcgcccact ccagcgtgcc ctggggatgg atcctcgtgc tggtggccgc gattgcggtg
     3481 ctcgctgcga tgtttcgtcg ccgcgccagc ccgacggtct acacgcagta cccgggcgga
     3541 accgccgctc cagtcggtcc gcccggttac gggccgggct acgcaggtcc cggatatggt
     3601 ccgcccggtg tgggctcggg gatcgccggt ggactggcga gtgggttggc tgtgggtgcc
     3661 ggcgtggtgg cgggcgaaga gatcgcgcac cacttcctcg atggaaaccg ggtcgagggt
     3721 aatccctacc cggtgaacga tccgcaggcg gataaccccg atatgggtgg ctcggacttc
     3781 ggcgtctccg acggcgggtc ctgggatgac ggcggctcgg tgggcggtga cgtcagcggc
     3841 ggcgacgact ggacctgacc gcacgtcact ttccttcagg agcctccggc ggtcagtgca
     3901 tcgaacagct gttgacctcg gggtaccccg aggcccgtgc aggcattcca gcccggacgg
     3961 gcctcgtatc ctagcgatgt gccggtcgcg gtgttatcgc cccgcgtgat gggacgcaac
     4021 agcgtagcgt gcgcataaag aaatggcagg cagaagccga tcgtatggcc agcgccctgg
     4081 ttgatcagcg cgatcaggcc cgcccacagg ggcgcgacag cactggtgcc accgacgact
     4141 cctggcttac ctcccacgag gatgcgatac ccgctgtcgg gcgcggcatc gccggctaca
     4201 tccggtacgc cgcggcgcgt cttcccgtcg ttcacgctgg ggggcagcga ggcgctctgc
     4261 tggaacgagg gcaccggaaa cacatcactg atgccacccc cggtaccgct gtcgccgtcg
     4321 ttccagacca cttcactcga gatcgaattg ccggaaaccg tgatcgtggt gccaccacag
     4381 ccgatcgccc agggactgga cgcagggaaa tccacgtgtg ccttgccatc ggtcacgccg
     4441 tcggtggcca gactatcgcc ggccgcgatg catacggaca cggctagtgt tgaggcatcc
     4501 tgcagtgcgc tattcatgct ctgcatggat tgttgagtcc aggtggactc cgcactgccc
     4561 cagctgatcg agaggacgcc cggcttgttg gtcgcatcgt gcacggcgct ggtaatggca
     4621 tcgagaaacc ctgcatcggt attcggcgcg aagtagaccg cgattttcgc gccgggcgcc
     4681 gcgccaccgg cgacctggat gtccagagcc acttcatcgt cggccccgtc atcggggctc
     4741 ggcgtgttgg ctcccccatc gaccggcact gcgaccacgc tcgggggagc aagacccatg
     4801 gccttgaagg cggcgctcgt atccgagttg gtataaccgc ctccgagctc gatgagcgcg
     4861 atgcattggc cggtcccggt gacgctggtg ggaaaaccat agagagcagt cacctggttc
     4921 ggcaggaagg cggcggccgc tgcggcccgc ggacgcgaaa ccagccgcgg ctgcgccgca
     4981 gggcgcgtgt cgagccccag cacggattca acaaccggaa gcagctccgc aggcaggctc
     5041 agcgagcccg tgcgtgcccg gaaagtccgc ttgccatcgg tgtagcggga aagcttggtc
     5101 tgaaatgcgg cctgagcctg gctcgccgtt ccggctagac ggaccaggcg tcggccgggc
     5161 tccgccgaca gcacggtcaa gccgtggtct cgggcgaagc tgcggagcag ttcgatgtcg
     5221 cccgcatgct gacttgcgcg tcgagcccgc tgtgcggcgc gcgcatcggt gcccggtgcc
     5281 gacgcagccg gggcgccatc gcgcggcttg aggtagatgc tgatctcgac gcgctcctcg
     5341 gcggggacct cacccacgag ccggcaggag ggccccggag cttcgcgatg actggcgctg
     5401 aattccttgt acgacttact ggccatgggc tctctcccgt gcggcgatcc ctcgggctat
     5461 cctaatgcag atagtgacgc accgggctct gtcgcccgat tgcgtcaaac gcgccgccct
     5521 gtgcgtcagc gcgcttgctc gtgaggaccc agtaacgcgt agcgccggcc atggggcagc
     5581 tcgtagcggt gaatgatcga gacgacgtgt tcgctcgaga gtgtgcctgc aacgccatct
     5641 ccgggcggct cggacggcat gatgcgatcg aggagccggg agagctcacc tggtccacga
     5701 cccggaagct tcacgagcac gtgtgctcgg ctgccgcgct cgcgaaacca actcgccacg
     5761 acaatttcgg ggctctgcgt gctgctgtcg agcaccgaga agggcaccgg taccgggagg
     5821 ctcgatgcag cctccggccc gcggccgcgg tcgacgatcg agaggggtcg atgatcgatc
     5881 atcgcgatcg tcgtctcatc ggggttcagc agcgcaagcg gggcgccgcg actgtcttcc
     5941 cggatctcac gggcaagcgc cggaagatcc tgccagcggt cgatcgtggg aaacatcgca
     6001 agcccaccga gcgtgagcgt cgcggcgtat gcgctatagg tccaggcgag cgagcgcggc
     6061 agatcgtcac ggccttgcat gaaggcggca aatttcagcg ccatgatggc gagcgccgcc
     6121 attgcggcgg cggtcgtgag gtaggtcagg tttccagaag ccactgaaag caggccgagc
     6181 agtgcgaccg ccgcggcggc gatcaagccg acgatggccc gggtgcctgc cagtgcaaac
     6241 aggtcgagac gcgtgggacg ttgggcgaga tgaccgacac tgatctccgt ggcccacagc
     6301 ccgatgagca gaccgaagcc gagcagtgcc ggcgcggcat agatgtcacg cgccgtggcc
     6361 gcgacggaca gcaggacgag aaagggcacg atcgtggcga tcgcgaatcg ccacgcggtg
     6421 ccctgaatgc cccggacgcg aactcgattc caggcgcgcc gcacggccgc gacggccagc
     6481 agcgtccagg gcaacagata caccggcagc tcgcgaaaat atttgccggg ggagttgtgg
     6541 tggccgctcg tgtagtcgag cgcggcggat cccgaaatcc gggtgaagcg tccgaaagtg
     6601 ttgtgccaga agagcgcact gagagattgc atgccatgtt cggtctggct cacggcatag
     6661 acccacggca ggatgatcaa cgcctgcagc aggaggcctg cgtaaagctc ccaacgcagc
     6721 agctcggacc agcggcgttc ccacacgatg atcgtgccaa gtgccagggc cggaaccaac
     6781 cagccaggcg cgctcttggc catgaatcca accgccgcgc ccgcatgcag gatggtatag
     6841 ccgagcagct tgcgccggcc cggctcggcg gtgaagccga ggtaggcgcc cagcagcgca
     6901 atcgcgcagc ccgccagcag acaggcatcc ggagccagcc agatcgccac acgataagcg
     6961 gtgatggcgg taccggccgc aatggccgcg accactgccg tcgagggtcc ctccattgca
     7021 aaagccagta cggcaatcgc gagcgcagtc aggaatgcgt acaggatgtc aggcagccgc
     7081 gccgccgcgg gactgtcgcc gtagatgtgc agagccagcg cagacatcca gtatgaaagc
     7141 ggtggcttct cgagaaaggg cgtttctgcc agatgcggca gcgtccagtc gtgttgcagg
     7201 ctcatgcgcc aggcgatgtc ggcttcgcgt ggctcgtcag gcgtccaggc gcctcggtcg
     7261 aggatgccga tgagccacag cggtgcgagt gcaacgatga tgaggagagg ccagcggcgc
     7321 acgatgatgt cgacccgcag aggacaacca cactgacggc gattatgcct gatcgggact
     7381 gagcgccgag ggccgctgta cggcgggcgt ggatgacgat gtcagtcggg cgatcaatcg
     7441 gcccgcatcg acgccgcggg catgcagacc gactttcgtg cgtctccaca ggatgtcttc
     7501 cgcggtactc gcgaactcct cattcacgag gtagtcgact tcccgctcgt aaagccccgg
     7561 catgagctcc tcacccatgt cggcgagcat cgaggcattg cccacgacac gctcgatgcg
     7621 cgtcccgtag gcgtgcgcgt agcggcggcg tacaccggcg ggcagccacg gatagcgtcg
     7681 ctccacggtc cgcagaaaag cagccaggct gccgtccggc aaatcaccgc ctggaagctt
     7741 cgcggcgtga gtccagggcc cgcgcgagca gccgagcacg tgggccagcc tgtccaccgc
     7801 actctcggca agcttgcgat aggtcgtgat cttgccgccg aacaccgaca gcaacggtgc
     7861 ggggattcga tccaactcga gggcatagtc gcgcgtgacg ctggagggat cggccgactc
     7921 atcacccaga agaggccgca cgcccgagta gctccagacc acatcggccg gtgagctcgg
     7981 atggcgaaaa tagcgattca ccaccgcgca aagataggcg atctcctgcg catcgatgct
     8041 caccgtgccg agcggaccgc gatagtccac atccgtcgtg ccgatgaggg tgaactcatt
     8101 ttcatacggg atggcgaaga cgatgcgccg gtcagcgttc tgaaagatgt aggcgaagcg
     8161 gtgtgtgaag agctgcggca cgacgatgtg gctgcccttg accagtctga ccgttcggga
     8221 ggcgcgcacg ggcgtgcgat cccgcacgag ctcattgacc cagggtccgg cggcattgac
     8281 tacggcacgg gcgcggacgg taagggtttg ctgcgcggtg gccacagttg cgagccagtg
     8341 atcgccttcg agatcgaggc gctcgcagcg cgtgcgcgtc aggatggtcg cgccgcgatg
     8401 tgcggcgtcg atcgcgttgt ggaccacgag ccgcgcgtca tcggtccaac cgtcagaata
     8461 gatgaagccg cgctgaaaac ccggcagcag cggctcaccc gcgacatgag tgcgcaggtt
     8521 cacggcggcc gaggcggcga gcagccggcg ccgggccaga tgatcgtaca aaaacagccc
     8581 cgcccgaatc atccaagccg gtcgaagatg cgcatcgtgc ggcatcacga aacgcaaggg
     8641 ccgcatcagg tgcggtgccg cccccagcag cacctcgcgc tcctgcagcg ctttgcggac
     8701 cagaccgaag tggaagtcct cgaggtagcg caggccgccg tgtatgagct tggtgctggc
     8761 ggaggaggta tgggaggcga ggtcatgctg ctcgcacagc aggaccgaca ggccgcgacc
     8821 ggctgcatcg cgcgcgatgc cggtcccgtt gatcccgccg ccgacaacca gcaggtcgta
     8881 tcgctcccgg agcgtgctca cacgctgcgc gaggcccgcg cctgattgta gtaatcggga
     8941 tagtcggccg tgaggccctt gaagcggcga tggatccaca gatattgctc agggacgcgg
     9001 cgcacctgag cctcgatcag atggttgaag cgctcggtgt cggcaacggc ggattcgctc
     9061 gggaagtcat cgaaaggcgg ataaatcctc gcgtgatagc ccttgccgtc gggcaggcgc
     9121 tcgaagaagt agggtaacac cggcgctccg gtcaggcgtg ccagtcgcga ggtgaaggta
     9181 ttcgtcgctg ccggaatgcc gaacagctcg accatctgcg cacccttctt gcggtagctc
     9241 tggtcggggg cataccagat gcactcgttg ttcttgaggg cgctgatgag cgtacggatg
     9301 tcattgagag gaatcggctg gccaccgtat ttgcagcgcc gatgcttgag gaagtgcgcc
     9361 agcacctcat tgcgggtcgg ccggtagagg aagctcaccg gcgccaccga cagcaggatg
     9421 cgaccgccga cctcgagcgt ggtgaaatgg gcggtcagca ggatggcccc tttgccacgg
     9481 gcgagtgcgg cctcgaggtg ctcgcgccca tcgatgtgga tcaagggctg ccagcgcggc
     9541 agcgagctcc accaggtgaa ggcgatctcg aacaggccga tgccgagact ttcgaagtgc
     9601 cgcaccagca gcttttcgcg ctcggcgggg ctcaactccg gcagacacag ctcgatattg
     9661 cggcgcgccg tgttgacgaa gctgagcggc aggtggcgca gcacagtgcc aatcggccgg
     9721 cccagctgca cgaggcggcg gtacggcagc agcgcgatca gccacagcag accgagcccg
     9781 atccacgtgg gccagtagcg cggcgcgaac aaccgaattg gcatcgaccc ccccggtttc
     9841 tccggcgtgc gagaatagct catccacgag ttggaactgt gactcaatgc ggcgatcgag
     9901 ccctgccgat gcgcgttacg tggaagtgag cctgcggcac ctcggcctgc gtaggggaga
     9961 ccgcgccatt ctcggggatg tcagttggag catccggccc gggcagcgct gggtgctcgt
    10021 cggaccgaac ggcgcgggga agacccagct tctcaaagtc gtctcgggcg cggtgtggcc
    10081 ggtgccgggg aagccctccc gtcgctatcg ctggcggcat gaagcctttc tgtcgccgtt
    10141 gggcgtgatg gatgagatcg gctacatcgg cccggaacga caggacaagt acgagcgcta
    10201 tggctggaac cacacggtca ccgagatcgt gggcaccggg ctgtaccgta cggatatccc
    10261 gctcgaccca ctgactgccc gggaccgacg gcagatcgcc gcactgctgc atagaatggg
    10321 aatcgagcac ctcggaccgc gacgctttct gtcactgtct tatggcgagc gacgcctcgc
    10381 gctgctgtcg cgcgtgctag cggcccgccc gaagctgctc ctgctcgatg agttgctgag
    10441 cggcctcgat gcgcagaacc gcagccgcgc tctgggctgg ctggagcgaa ccgggcgggc
    10501 ccgcttgccc tgggtgctca ccgcccatcg gctcgaggac gtgcctcgct ccgcaaccca
    10561 cgccctcgtc ctcaacgcgg ggcgcatcaa gtatcgcggg tcagcgcgcg gtgcgccgct
    10621 caagcggtgg ctcgttcagg gcaaagccga tcgtgcgcag ctgggaggtg cccggcgcag
    10681 tgtcgatcgg ttgttgctgc gactgacccg cgcatcggtc tatgtggatg agcatcgggt
    10741 tctcgaggct ctctctttca ctctgcgctc gggtgagtgc tgggtggtgc atggggcgaa
    10801 cggctcggga aagagcacgc tgctgcgaac cctttacggc gatcaccggg ttgctgccgg
    10861 cggccggatc gagcgcagcg gtgtggcagc gggagtcccg ctggagttgt tcaggcgccg
    10921 agtcggcctc gtggcgcccc atctgcagtc cggccatcct catcagctca ccgtgagcga
    10981 ggtcgtacag tccggtcctc acgccagcat cgggctcggc gctgcagccc gctgtcccga
    11041 tcgcagggcg gcgagccatg cgctcgagct gttcggtttg cagcggctgg ctgcgagagc
    11101 cttgcatgag ctctcgtatg gtcagctccg gcgggttctg tttgcccggg cgtgggcgtg
    11161 ccgtcccgca ctcctgctgc tcgatgagcc gtatgccgga gtcgacgccc gcacgcggcg
    11221 cgacctgatg catctggtcg aagggatgct cgcgagcaat acggctgtgg tcatggccac
    11281 tcaccaccgg cgagagtggc cgaccttcac gacgcacgag atcgagctgt ccgccgggcg
    11341 cgcactctac tgcggtccgg tacgatcgcc tctggagagt cgcacgcgag acggagcaga
    11401 tccttgagaa tcgttgtggg atgggcagtt ctggtcctcg gcgtgctggc cgtgctcggg
    11461 atcgcgcacg agccgctgtt ctgggtccat tacgcgctga cacggatcca tggcacacac
    11521 ttgccgctct tctactacga gccccgcgag ctcgttcccg gcggcaacga gccgcccccg
    11581 ccacgtgagt cgcccgcctc cgagtcgctc gacatcggag cgctggaggc tgctgcggcc
    11641 tatgccggcg cgcatcactc ccgcgcgctc atcatcagcc ggcatggcta tctcatctac
    11701 gagcgttact ggcacggtag caggttcgac acgctgatcg agtcggggcc gctcgggcga
    11761 gtgctggcag cgctcgtgac gggcaacgcc atctacggtc gtcgcatcgg ctggccggac
    11821 gcaccgatcg ggatgttcct ctccgagtgg caggacgatc ccagagggtc gatcacggtg
    11881 cgcaacttgc tgcaatcctc cagcgggctg gccgtgccgc ccccggctgc gaatccgtgg
    11941 agcgcggccg ccgatcaggc gtacgggagc cacgtgatcg cgggatattt gcaacgctca
    12001 ctgcagagcc ctccggggca gaagtgggtg cagcagagcg tggaccctga tctgttggtc
    12061 ctggtgatcc agaaagcgac ccacgagcgc tatgcccagt atctgtcgcg gactctgtgg
    12121 cgccggatcg gcgcagcgga tgcgtggctg tggctcgatc gtccagaggg agacgtgcac
    12181 gtggatcgag gctttctggc gcggcagggg gactggctgc gcgtggcgga gcttctgctg
    12241 cagaacggcc gctaccaggg ctccgaagtc atcgatccgc gctgggtgcc gcagctgctg
    12301 cggccagcaa aagccaatgc cgattacggg gcttacatcc ggctcggagc ccatacctta
    12361 cccggtatga ctgcctacga cgcaccggat ctgttcctcg tccagggcgg gggcagctat
    12421 ctgtggctca ttccctcctt acagcttgca gtcctgcgca cgggtgaagc gcccccggcc
    12481 gagcaggcgg gcaggaccgc gagtgacgac agtgcgtggg atgaggcccg gattcccaac
    12541 ctcgtcctgc acggcacgcg ggacttcgta cccgcggcgc gctcgggcgc gggtatctcc
    12601 aacatcgtgc cgaatcattg agcccgccgc gctccgtgca atctgaagag gatccgcggg
    12661 ctcgctctgc cgtgagaaaa caactcggct cgctcttcat cgtcaccgtg gtcgacatcc
    12721 tgggattcgg gatcctgatc ccgctgatcc cctacatggg catgaagttc aacacgccgc
    12781 ccgaatggat cacgcctgtc atgggcagct actcgctgtg tcagctcatt gccgctccgc
    12841 tgtggggacg gctcagcgac cgctacggcc ggcggcccat cctgatgagc agtctcgccg
    12901 gagcctgttt gtcctacctg ctgctggggt ttgccaccaa catctggtgg ctgctgttgt
    12961 cacgggccat caacggcttc atggcgggga acatctcggc cgcctttgcg tacgcctcgg
    13021 acgtgagcga gccgcgccgg cgtgcgggcg cgctggggct gatcggagcg gcgatcggaa
    13081 ttggcttcac gctggggccg gcgatcggcg ggatcctcgc cggcgacgat ctgcgcaccg
    13141 cgagctttct gcgcccggcc atcgtctcgg cctgcctgag cagctgtgcc ctgctgctgg
    13201 tcgcctgcgt gcttcccgag agccataccg agcggcatcg ggccgagcat ccgctcgggg
    13261 cgcgcatcgg cccgatacgg ttgctgctgc agcgccccgt gctgcggttg gtcgccgccg
    13321 cggcgctgct ggtgaccagt gcgcaatcca ttctcgattc cattctgggc atctgggcgc
    13381 tcaatcgcta cggctatggc ccgcgcactt tcggcgtgct gatgtttggt attgcattct
    13441 tcgccatcct cacccaaggg gtcctggtgc ggtatctggc gccacgcctc ggcgaatcga
    13501 agctcgcgac gctcggcgcg ctgacgttcg cagcaggact gctgatcgtc gctctgacat
    13561 ccgtacctga gatgaccgtg gtgggacttg cgctgtgcgg catcggtgtg ggtgcgttct
    13621 ctcccagcgc ttcggcgctt gcttccaggc aggcggcctc ccatgaccgc ggcgcagtga
    13681 tgggaacgta tcagtccgcc agcagtctgg cccgggttat cggccctttc acctcgggtc
    13741 cggtgtatgc agcctgggga acgagtgcgc cgtttctggc cggggcgtgt gcggtgattc
    13801 cggcggtgct gctgatctgg cgtacccggc gcatcgacgc gcagcggacc gcggctactc
    13861 gctgatactc gcgccggcgc tcgccgggct gacagcgact tcgatcatgc gggcgacgat
    13921 tcgcacggga aagctcggca gcggcagctc cgccggcggc cccaacacct tgccatcgcg
    13981 gatgtcgaac gatgcgccat gcagtgggca gacgatgcgc gtggccttca accagccctc
    14041 acacatgcgc gcatgcgcat gggtgcagat gttgtcgagc gcgaagacgc cctcacgggt
    14101 gtgacagatc aggatctcgc gcgccgccac cgtgtaggcg cacatgctgc cccgccggat
    14161 tccggtcagc ggcagaacgg actcgaaccg caactcggtc acagagcgat ttcggtttcg
    14221 aagatggaca ccgcctggcc gatgatccac accccttcga tacttccatc cttgagctcg
    14281 agttcgacgt tcacccgccc cggccgatgc agctgatggc cctggatgcc ggtgaaggaa
    14341 acccgggtgc cttcgcgcgc cagcaacccg gtgcgggcca gatagattcc gagcagaccg
    14401 tgcgcattgc cgcttaccgg gtcctcggtg atccccaggg ccggacagaa catgcgcgac
    14461 tcggtgaggt gcgcgccgct gccgctgctg agcgcgaaca cgaaatagcc cgccgcgccg
    14521 acctgcgccg agagggtcgt cagccggttc acgtcgggct tcaggtgctt gagcacatcc
    14581 gcgctgcgca ctccgatcaa caagcgtgtg ctgctcgagc cgacgatgcg cagcgggcag
    14641 cgcgggtcga ggtcgtccgt ggcgagcgcg agagagtcga gcacggcgag tcgctcacga
    14701 tcattgagct cgcggccgat cggtggagtg ttctggcgga tcgcgatgct gcggtcctcc
    14761 tcctgccccc tgacctcgat gtccacgatg ccggctttgg atttctgccg ctgccggcgc
    14821 ggcgcgttgt gccgacgcga gagtacgtag tgggcagcca ccgaggcgtg tccgacgaat
    14881 cccacttccg tgcgcggcgt gaagaatcgt gcgcgcagcg tgtgatcatc gccatcggag
    14941 ttgaggatga aggcggtgtc ggcgttgttg agttcgcgtg cgatggccag catctgggcc
    15001 tcggtgagcc cgtccgcgtt gagcacgact cccgccgggt tcccggtgaa gcgctcgcga
    15061 gtgaaggcgt ccacctggtg cacctggacc tttctagttg tcatcgcctg attgtaatcc
    15121 catcggagag gtgtggggcg cacgaggcgc ggcggtcgcg catcgaagtg ccggccggtg
    15181 cggttgcgtg ggaatgcggg cagacgatgc attttcgcgc cgagccgggc ggtcgatgca
    15241 ttttcatcgc ggagtgataa cataagaacg cacagccttg gggtggtccc cgtgtggtgc
    15301 gcgaattccg ccaggaactc gcgccgccgc cgcaggacct gagacgccgg tgagccggcg
    15361 aacccgttga acctgatccg gttagtaccg gcgtagggag caggtggatc ttgcgctctt
    15421 tcccatcggc ggtttttctg ctggccccct gcgccctggc gctcgcctcg cccgagtccg
    15481 agccaccgct gcaggaagtg gtcataaccg gcacactgcg tcccacttcc gagcaggacg
    15541 ttccggccag cgcctcggtg ctggactccg ccacgctcga gcaggccggg gagcagcatc
    15601 tggaggatgt gatcgggctg gtacccaatc tcaactgggc gggcgatacc tcacgcccac
    15661 gatactttca gatccgcggg atcggtgagc tcgatcagta tcagggcgcg cccaaccctt
    15721 ccgtcggctt cctgatcgat gacatcgact tcagtggcct cgggaccgcc gctacgctgt
    15781 tcgatattga gcaggtcgac gtgcttcgcg gtccgcaggg tacccgctac ggtgccaatg
    15841 cgctcggcgg cctgatctat gttcagagcg cagcgccgca attcacatat ggtggccgcg
    15901 tggagctggg ggccggcgat ttcggaatgc gctcgtatgg agcggttctg accggcccgg
    15961 tggacgagct ggactcggcg ttccggatcg ccgcgcagcg ctacaccagt gatgggttct
    16021 atcacaacgc atacctgcac cgagacacgg acgacaatga tgagctcacc ttgcgtgctc
    16081 gctggcgctg gcggccctcc gaccagctcc gtgtggacct caccgtcctc gatgtgcaca
    16141 tcgacaatgg ctatgacgcc ttctcgatcg acaactcccg cacgacccag tccgaccagc
    16201 caggggagga tgtgcaacat tcgaccggtg tctctctcag ggcggattac acggggctcg
    16261 gcccgatgac actcaccctc atcggcacac tggccaacac cgggattcgc tacggcttcg
    16321 actcggactg gggcaacccg atctcctggg cgccctacac gtacgagttc accgacaacc
    16381 agcgacgcga ccggcacacg cacagcctcg agctccgcct cgccagcacc ggagagcatg
    16441 cgctcgactg gctgttcggg ctgtatgctc tggacctcag cgaaggcttc aacgacacca
    16501 gtccgggcct gtatctcgat ccctttgatc cgacccagga cgcacacacc ctcgcaatca
    16561 cgaccagtca ctatcattcg cgcaacgagg ctgtcttcgg cgagctcgat ggcgctctca
    16621 cgcggcgctg gcactggact gccgggctgc gcgccgagcg gcgtatagcc gactatcacg
    16681 atctcacgac caacctcggc gagcccgatg ctacccacgc cttctctccc gtcgatcaca
    16741 tgtggggtgg agatctctcg ctccagtacc gcctcgccgc cgagcagcgc ctgtatgtgc
    16801 agctcgcgcg cggctacaag gccggtggat tcaacctggg gcccggactg ccgactgacc
    16861 agctgctgtt caacccggag tcggacgtga acctggagtt gggatacaag gcggatctcg
    16921 ccgagcaccg agtgcggatc gacgccgatg tgttctacac gcatcgcacg ggactgcagc
    16981 tgcagaccgg tgagcagctg gtgccgcagg atccgacgac cttcattctc tatacggcca
    17041 atgccgcgag cggccacaac tacggcctgg aatcgagtct ttcctggcag ccgatcagcc
    17101 tgctcgagct gctcgcctcg gcgggactgt tgaacaccgc ctatcacggc ctgacgctcg
    17161 atggcgaaag gctgcccgat cgggccctgc cgcatgcccc gagctggcag gcggcgctca
    17221 gctccattct gacgttgcga cagggctact tcctgcgcct ggatctcagc ggcaagggca
    17281 gcttttacta cgacgtgccg gcgctcgacc cctacgcctc gagcgcttac atgctgctga
    17341 acgcaaaagc gggcgtgcag cgcgagagct ggggtgccaa catctggatg cgcaacgtca
    17401 ccgaccggcg ctataccgtg cgcggattct atttcggcga cgtgccgccg gatttcccca
    17461 gccaacagta cgtgcagctc ggcgaaccac gctcgttcgg ggcgcagatc acctactcgt
    17521 ttcactgagc acagaggcat ctggaggtca tcatggaaat cggagtcgag atcagtcttt
    17581 atccgctcaa tcagcacttc attgcgccca tccaggactt catcgaccgt ctgaatgccg
    17641 acgggcgttt caaggtcgtg accaacagcc tgagcacgca ggtgttcggc cgctacgagg
    17701 agatcttcga cagcctgacg cgcgagctgc gcatcacgtt cgagaacaac gacaaggcgg
    17761 tgttcgtcat gaaggtcatc ggtccgatga catgatcgac tggcggcacc tgctcacgag
    17821 cctggttgcc ggtctggaag cgaccacggc accggaggca gccgcggtgg tgctggggct
    17881 gacctattcg ctgctcgcga tccgccgcag ccgctggtgc tgggtggccg gcggtgcgag
    17941 ctcggcgatc ctcattggcc tctcgtgggc acgccatctg ccgatgcagg ccgggctgaa
    18001 catctactat gtcgcggtgt cggtgtacgg atggtggcac tggtcgcgcg aacaggccga
    18061 gcagggcagt ccgcaggtca gccgatggcc gctctacaaa cacctgctgg cctgcctggg
    18121 tgtggtgctc gcgagtgcgc tcactgcccg atggctcagt gccgagaccc aggccgcctg
    18181 gccgtttctc gaccccctga ccacctgggg cagtctgctc gccacctggc tcgcggcgcg
    18241 ggtgaagctc gagaactggc tctactggct ggtgatcgac tcgctgctcg cgttcctgtt
    18301 cggctcccag ggactgcatt tcgtggcgct gctctccatc gtgtacctgg gcttctcgac
    18361 ggtcggattt ctgcgctggc gcaagagcta tcgggccctg gcatgcgcga actgatgcac
    18421 gaggcggggc tcgaagcggc cctggcgcac gtgccgggtt atgttccggg gcgctgcgag
    18481 ggcaccgtca ggcgcttgtc gggtggcagt gtcaatcgga gctatcgggt atgcacgcct
    18541 gcggggggtt acgtcgtacg cctgagcccg gtgacggatg cgtggctcgc ggcggaccgt
    18601 tcggtcgagc gcgagctgca tgcccgggct gccgctgcgg gcatcgcgcc gcggatcgtc
    18661 cacgccgacg ggaacgatcg ctggctgatc accgagttgg tcgaggggcg gttgtggtcg
    18721 gaggcggact tcggcaagcc ccactctctg ggcttgctcg ctgacaccct gagcgttgtg
    18781 catgctctgg agccgcccgc aggtgctccg atcgacctgc tgggcacgat cggcgcctac
    18841 gcgcggcaac tccagccagc cgatccgcgg ctcgagggct ggctggatgc ggcggcgcgc
    18901 gcttggccgc tctcaggggc gcctgagcgt caacccgcca tcgtgcatca cgatctgcac
    18961 gcctcgaatc tcatcgaaac gggcgcaggc ctgcggctca tcgactggga gtgcgccgcc
    19021 gtcgctgatc ctctgctgga tgtcgcctgc attctctcct actacgagac cgcctggagc
    19081 tgtgccgcgc tgctgctgga gcgggcgggg ctgcgtggca tcgatgcgag ccaacttcgg
    19141 gcttcggtct ggctgttcga tctgcacacg tggctgtggt attgcgagag gcgccagcgc
    19201 ctctcgccga cgcaggcgga gctcgatgcc gagcaacgcc tgtcggcgcg cctgatgcga
    19261 ggcgtggctt agctcgcagc gcaagcttgc tacgctgggc tcgactggcg ccccgggcgt
    19321 cgaatcggca cactagcccc tgcggccgct ctgctgtttg cggctacccc ttcagttgtc
    19381 gaggtcgtag ttcatggcgg ttttgcgtgt ggtcgatcgc gatggagtcg agcacgaggt
    19441 cgacgcgaag accggcctga aggtcatgga gacgctgcgt gagctggact acggggtggc
    19501 agcgatctgc ggcggcatgt gctcctgcgc cacctgtcac gtgtacgtgg atccgcagtg
    19561 gctcgccaaa cttccagctc cgatgtccga tgagcgcgag ctgctctcgg agctgtcgca
    19621 tcacgaagtc aactcgcgct tgtcctgcca gatcgagttc acgcaggccc tggcggggct
    19681 gcgtgtcacc attgcccctg acgagtaggt cgcctcaatg tccacttcca gtgtccaggt
    19741 cgagactcga ggtgccgtag ccctcgtcac gctcaatcgg cccgacaacg ccaatacact
    19801 gaacctgcag accgcgatgg acctgctggc ggcggccatg acctgcgatc gtaatcctgc
    19861 cgtgagggcg gtcgtgctga ggggtgcggg gcgtcacttc tgtttcggag gcg
//
LOCUS       DASRHN010000179         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2039382, whole genome shotgun sequence.
ACCESSION   DASRHN010000179 DASRHN010000000
VERSION     DASRHN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039382"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..556
                     /locus_tag="VFB37_06985"
     CDS             173..556
                     /locus_tag="VFB37_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZO22235.1"
                     /translation="MDHQITDLGELEREVLELVWAHGPISADAVQKRLARALKESTVR
                     TVLKRLEEKGYLGHTTENRTFLYTAKQTRSSAAARAVKGIVDRFCHGSVEEVLVGLVD
                     AQILDRRELARLAEKIAKTKDGGKS"
     gene            553..1842
                     /locus_tag="VFB37_06990"
     CDS             553..1842
                     /locus_tag="VFB37_06990"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZO22236.1"
                     /translation="MIRLVVEATLRSCALGLALWLLLTLTRSRNPHLHKLMGAAMLVA
                     SLAMPLLMQVRMMPVIHAPDSVLTLQADPVGATHLGAAWNSIDAPYIDALYVLVALAL
                     LARYLRSLLTMWHVRRDAELVDYTSLARLEMGSLDVRVTSRVRSPSTFGSTILLPTDF
                     VEWPHQKLTAVLAHERAHVLHKDCYMLWLARLHTCVFWFNPLAWWLQRKLAALSETTS
                     DEAAVQAIGDKPMYAEILLEFAACRAESDVATAMARPHIVTRIERVISEITPSAIPKL
                     SQRILAIAAVLPMVAAAAAPLGQSPVHSTQSGAPPDAAICQGKASQQPCIKTGHPTLA
                     ELAKYYPREAARRGIDGLVHIQVTLDTQGHATDTLILSEDPLNMGFGAAASALAHVME
                     YNNPTDRPAQLAFNVKFALAQPTPSYGTTNFESPDTP"
     gene            2045..>2670
                     /locus_tag="VFB37_06995"
     CDS             2045..>2670
                     /locus_tag="VFB37_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZO22237.1"
                     /translation="MMDPHHHEHDHSVDVGHAHDHGDHHHGHHHHHHHHHGEEGSERR
                     LIVALLILGSFTVVEAVGGWFANSIALLAEAAHMLADSGSLFLAIVALRVSRRPANEK
                     RTFGHRRYQPLAAFINGQALLLLTAWVVYEAITRLAHRPEVNGQLMLAIALIGGVANL
                     AAFMTLSGARSLNERGARVHILSDLVGSGMASVAALLVLTKGWAVADP"
BASE COUNT          504 a          893 c          802 g          471 t
ORIGIN      
        1 tcatcgcgcc tggcgtctca catgattgga ctcagccggc cacgctgccg caggtccagc
       61 gccgtatcgc cgactggttc aaccattatc tctagagagt cacgccctga gccgcttgcg
      121 ctctacattc agtagcgcta cgatctgtag cagtacgact ggtagcacca acatggacca
      181 tcagatcacc gacctcggcg agctcgagcg cgaagtgctg gagctggtct gggcacacgg
      241 cccgatatcg gccgatgcag tccagaaacg actcgctcgc gcactcaagg aatccaccgt
      301 gcgtaccgtg ctgaagcggc tcgaggagaa gggatatctc gggcatacga ccgagaaccg
      361 caccttcctc tacacagcca agcaaacccg ctcgagcgcg gccgcgcgcg cggtgaaagg
      421 gatcgtggat cgcttctgcc acggctcggt ggaggaagtg ttggtcgggc tggtggatgc
      481 gcagatcctg gaccggcggg agctcgcccg cctggcggaa aaaatcgcca agacgaaaga
      541 cggaggcaag tcatgatcag gctcgtcgtc gaagccaccc tgcgctcgtg cgcgctgggg
      601 ttggcgctct ggctgctgct cactttgacc cgctcgcgca atccacatct gcacaagttg
      661 atgggagccg cgatgttggt tgcctccttg gccatgccat tgctcatgca ggtgcggatg
      721 atgcccgtga ttcacgcccc tgactccgtt ctcacgttgc aagcggaccc tgtcggagca
      781 acacacctcg gcgcggcctg gaatagcatc gacgcgcctt atatcgacgc gctctatgtg
      841 ctggtcgccc tggccctgct cgcgcgttac ctccggagcc tgctgaccat gtggcacgtc
      901 cggcgcgatg ccgaactcgt cgactatacg agccttgcgc gcttggagat gggtagcctg
      961 gatgtgcgcg tgacctcgcg cgtgcgaagt ccgtccacct ttggatccac cattctcctg
     1021 ccgaccgact ttgtcgaatg gcctcaccaa aagctcaccg ctgtcctcgc gcacgagcgg
     1081 gcgcacgtcc tacataagga ctgctacatg ctgtggctcg cacggctgca tacgtgcgtc
     1141 ttctggttca atccactggc ctggtggcta caacgaaagc tggccgcgct gtcggaaacc
     1201 acgagcgatg aagcagcggt acaggccatc ggcgacaaac ctatgtacgc tgaaatcctg
     1261 ctggaattcg ccgcgtgccg agccgagagc gatgtcgcaa ccgcgatggc acggccccat
     1321 atcgtgaccc gcatcgaaag agtcatttcc gaaatcacgc cgtccgccat cccgaaactc
     1381 tcgcagcgca tactggcgat cgcggcagtc ctgccgatgg tggccgccgc ggcggctcca
     1441 ctggggcaaa gccccgtgca ctcgacacaa agcggcgcgc cgccggacgc ggcgatctgt
     1501 cagggtaaag cctcgcaaca gccctgtatc aaaaccgggc accccacgct ggcggagctc
     1561 gcgaaatact accctcgaga agcggctcgg cgcgggatcg atggcctggt ccacatccag
     1621 gtgaccctcg atacgcaggg gcacgcgacc gacacgctca ttttatccga agacccgctc
     1681 aacatggggt tcggcgccgc ggcttcggca ctggcgcacg tgatggaata caacaacccg
     1741 accgaccgtc cggcgcaact cgcattcaac gtgaagttcg ccctcgctca gcccaccccg
     1801 tcgtacggca caaccaactt cgagagcccc gacacaccct gagcgcgcag ctacgcccgc
     1861 agccgcccgg tcagcgccgg gcggccactt ccaaaaggac agcggcctct taccctaaga
     1921 ggtaccggct gggcaactgg aacgccaaaa tgggggagat tagactccgt gccatgatac
     1981 gaatcagcat tgcgatgccc agcgccggct tccagccggc cgaatgcgcc cgccggccgc
     2041 tcgcatgatg gacccgcacc atcacgagca cgaccactcg gtcgacgtgg ggcatgcgca
     2101 cgatcatggc gatcaccacc acggccatca tcaccaccac catcaccatc acggtgaaga
     2161 aggtagcgaa cggcggctga tcgtggctct cctgattctg ggtagcttca ccgtggtgga
     2221 agcggtcggc ggttggttcg ccaattcgat cgcactgctc gccgaggcgg cgcacatgct
     2281 ggccgactcc gggtcgcttt tcctggcgat cgtcgccctc cgcgtgagcc ggcgtcccgc
     2341 gaacgaaaag cgcactttcg gccatcgccg gtaccagccg cttgcggcgt tcatcaatgg
     2401 acaagctctg ctgctgctta cggcttgggt ggtgtacgag gcgatcacgc ggctagcgca
     2461 tcggcccgag gtgaacgggc aattgatgct cgccatcgcg ctgatcggcg gtgtggccaa
     2521 cctcgcggca ttcatgaccc tctccggtgc tcgctccctg aacgagcgtg gtgcccgcgt
     2581 tcacatcctg agtgatctgg tcggctcagg aatggcgagc gtggcggcgc tgctggtcct
     2641 caccaagggc tgggcggtcg ccgatcccat
//
LOCUS       DASRHN010000180         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1708579, whole genome shotgun sequence.
ACCESSION   DASRHN010000180 DASRHN010000000
VERSION     DASRHN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708579"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="VFB37_07000"
     CDS             <1..1603
                     /locus_tag="VFB37_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZO22238.1"
                     /translation="RQAVDAFERIADGEYDNMLDTRGRDEASQVLRALQQMQSRLRSQ
                     IETERAQAAENTRIRQALDKASTSVVLANASHEIIYANETALATFAAGQNEMRKSLPQ
                     FDARHLRGTLLESLATQSARERQLLETLSAPDSQERRLGAMTFHTVSSPVVDANGRRI
                     GTVVEWTDRTQEVGTEQQMQSVIAAVLDGDLQRRIPLQGMSGFFAAASRKVNQLADNM
                     AEIVALVKGSAAQIHGGAKEITGGNTNLQMRTEEQASSLEETASSMEEMTTTVKRNAE
                     NASQANQLAVAARGQAEQGGMVVGKAVHAMSDINESARRIAAIIGVIDEIAFQTNLLA
                     LNAAVEAARAGEQGRGFAVVASEVRTLAGRSATAARQIKELIHNSVRKVEDGSVLVTQ
                     SGQTLEQIVTSVKKVSDIVAEIAAASREQSAGIGQVNQAVLQMDELTQQNAALVEQAT
                     AASQAMAQQSQALHEMMSGYRLGERSGMDEAALSAQTPASPQDAAGQPAAGSAHEPRE
                     LADRLSMHPGVAAGADAGKAAWQDF"
     gene            1633..>2620
                     /locus_tag="VFB37_07005"
     CDS             1633..>2620
                     /locus_tag="VFB37_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22239.1"
                     /translation="MKFLSRLKLWQKLAVLVLAMAIPSALLGIFYLSGANAQVALAQN
                     EIEGAHYVQAVGAVLAELANHRGRLFAVLAGDSAQADELAASDLAMTRDLAAVDASDA
                     HVGGRFKVTQAWQGIRSDWERLQAEGPKLPPDEAVGRHTELIQRISKLGEKIATRSGL
                     NVDPSPQTSSLIQIATRDVPEALIQSGDVRWYAARASIKGYLGGDDRMAIQLYHDAYT
                     EAFDSAARELERASDEAKARVQPRIEAARSAFDSFFAIVKTRILDAQKMDISTSEIFA
                     QSSNVSTGLKELSDVSYATMTAAVEQRLRAVTTWRNLTAGITALALAFALALS"
BASE COUNT          491 a          855 c          875 g          399 t
ORIGIN      
        1 tcgtcaggcg gtcgatgcct tcgagcggat cgccgacggc gagtacgaca acatgctcga
       61 tacccgcggc cgcgatgagg ccagccaggt gttgcgcgcg ctgcagcaga tgcaaagcag
      121 gctgcgctcg cagatcgaga ccgaacgcgc gcaggcggcg gaaaacaccc gcatccgcca
      181 ggcgctcgac aaagcttcca ccagcgtggt gctcgccaac gcgagccacg agatcatcta
      241 cgcgaacgag accgcgctgg ccaccttcgc cgccggccag aacgagatgc gcaagagcct
      301 gccgcagttc gatgcccggc atctgcgggg cacgctcctg gagtcgctgg caacccagag
      361 cgcacgcgaa cgccagctgc tcgagaccct cagcgccccg gattcgcagg agcgcaggct
      421 cggtgccatg accttccata cggtgagcag cccggtggtc gatgccaacg ggcggcgcat
      481 cggcaccgtg gtggagtgga ccgatcggac tcaggaggtc ggcacggagc agcagatgca
      541 gagcgtgatc gccgctgtgc tcgatggtga cctgcagagg cgcattccgc tgcagggcat
      601 gagcggcttc ttcgccgccg cgagccgcaa ggtcaaccag ctcgccgaca acatggccga
      661 aatcgtcgcg ctggtgaagg gatctgccgc gcagatccac ggaggcgcga aagagatcac
      721 cggcggcaac accaacctgc agatgcgcac cgaggagcag gcctcctctc tcgaggagac
      781 cgcatcctcg atggaggaga tgacgaccac ggtcaagcgc aacgcggaga acgcaagtca
      841 ggccaatcag ctcgcggtgg cggcgcgcgg ccaggccgaa cagggcggca tggtggtcgg
      901 caaggccgtg cacgccatgt ccgacatcaa cgagtccgcc cgcaggatcg cggccatcat
      961 cggcgtcatc gacgagatcg cctttcagac caacctgctg gcgctgaatg cggccgtgga
     1021 ggctgcccgc gccggcgagc aggggcgcgg cttcgcggtc gtcgcctccg aggtcagaac
     1081 tctggccggt cgctcggcta ccgcagcgcg gcagatcaag gagctgatcc ataacagcgt
     1141 gcgcaaggtg gaggacggat cggttctcgt cacgcaatcg gggcagacgc tcgagcagat
     1201 cgtgacttcg gtgaagaagg tctcggacat cgtggcggag attgccgcag catcccgcga
     1261 gcagtcggcg ggcatcgggc aggtcaacca ggcggtcctg cagatggatg agttgaccca
     1321 gcagaacgcg gcgctggtcg aacaggccac ggccgcatcg caggcgatgg cccagcagtc
     1381 gcaggcgctg cacgaaatga tgagcggcta tcgcctcggc gagcgctcag gcatggatga
     1441 ggcggctctg tccgctcaaa caccagcgag tccgcaggat gcggccggac agccggcggc
     1501 aggctcggcg cacgagccgc gcgagcttgc ggatcggctc tccatgcacc ccggcgtggc
     1561 cgcgggtgcc gatgcgggca aggcggcctg gcaggatttt tgaccggttc agccggacaa
     1621 aggcagaagg acatgaagtt tctcagtcgc ttgaaactgt ggcagaagct ggccgtgctc
     1681 gtgctcgcca tggccattcc gagtgcgctg ctcggaatct tctatctcag cggcgccaat
     1741 gcgcaggtcg ccctcgctca gaatgagatc gaaggggccc attacgtgca ggcggtgggc
     1801 gccgtgctgg ccgagctcgc caatcatcgc ggcagactct tcgccgtgct cgccggagac
     1861 agcgcgcagg ctgatgagct cgccgcttcc gatctggcga tgacccggga cctcgccgcc
     1921 gtggatgcca gcgatgctca cgtcggaggc cgcttcaagg tgacccaggc ctggcagggc
     1981 atccgctccg actgggagcg gctgcaggcg gaaggaccga agctgccccc tgatgaggcg
     2041 gtcggccgac acaccgagct cattcagcgc atttccaagc tcggcgagaa gatcgcgacg
     2101 cgttccgggc tgaatgtcga tccttctccc cagacctcct ctctgatcca gattgccacc
     2161 cgcgatgtcc cggaagccct gatccaatcc ggtgatgtgc gctggtatgc agcgcgcgcc
     2221 agcatcaagg gctatctcgg cggcgacgat cgaatggcca tccagctcta tcacgatgcc
     2281 tacaccgagg ctttcgattc cgccgcgcgt gagctggagc gtgcctcgga tgaggccaag
     2341 gcgcgtgtgc agccgaggat cgaggcggct cgctccgcct tcgactcctt tttcgcgatc
     2401 gtcaagactc gcatcctcga tgcgcagaag atggacatca gcacctccga gatctttgcg
     2461 caatcgagca acgtgagcac cggtctcaag gagctgtcgg acgtcagcta cgcgaccatg
     2521 acggccgccg tcgagcaacg cctgcgagcc gtgacgacct ggcgcaatct gactgccggt
     2581 atcacggccc tggccctcgc tttcgcgctc gcgctttcct
//
LOCUS       DASRHN010000181         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_227068, whole genome shotgun sequence.
ACCESSION   DASRHN010000181 DASRHN010000000
VERSION     DASRHN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227068"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFB37_07010"
     CDS             complement(<1..461)
                     /locus_tag="VFB37_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22240.1"
                     /translation="MRARFLPMTLALVLSGPFCAGAADRSLSVTIYANDLALIQDTRD
                     IQIAAGRQRIEFQDVAAKIRSETVSLTCSDLSIVEQNFDFDLLSPSALMQKAVGETIT
                     LVRTNPATGQETREQAKVLAVNGGTVLQIGNRIEVLRDDGLPVRVVFDRVP"
     gene            600..1649
                     /locus_tag="VFB37_07015"
     CDS             600..1649
                     /locus_tag="VFB37_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO22241.1"
                     /translation="MKIAQIAPLDESVPPQLYGGTERVVAHLCDALVDRGHEVTLFAA
                     AGARTRAHQVSARDQPIRLDKSELKSELGAHLSMLHDVRERAGQFDILHFHTEMLHFP
                     MFEQLAHKTVTTLHGRLDVKDLPGVYSRWPQFGLVSISNDQRRPLPHAHWLATVPHGL
                     ELDLYRFQERSRPGYLAFLGRISPEKGADAAIRIASKAGLPLKIAAKVDDADAVYFDS
                     EIKPLLENQAVEFVGEIGDEQKSQFLGDALALLFPVDWPEPFGLVMIEAMACGTPTIA
                     WQRGSVPEVIDEGVTGLIVNSIEEAVAAIPRVRQLDRQRIRATFERRFGAETMANAYL
                     DVYNRLLASRPTAAP"
     gene            1668..>2945
                     /locus_tag="VFB37_07020"
     CDS             1668..>2945
                     /locus_tag="VFB37_07020"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HZO22242.1"
                     /translation="MSSANEESAERQGLLPRFTLKDADMFLLSDALGDIQSSRDGLFS
                     NDTRMLSRYELQIAERRPALLGAALSQDNTMLTSHLTNLPLPILGEGVIPRGVIHIER
                     SRLLWQGSLHERLKLTNFGNLVAQVPLRFCFDADFADIFEIQGTVRQRSGARQPPEID
                     GRTVRLGYRGLDERERVTYLAFSRVPQVLETHEARFMFELHNREPVELFVEISTAAQP
                     APAAERFAAAAEGAAAKMSQRVAAAATLRTSGRLFDEWINKSRSDLALLTTDLATGPY
                     PYAGIPWFATQFGRDAIITALQTLWTQPRLAAGVLRFLASTQARETSAFRDSEPGKIM
                     HESRRGEMAALGEVPFGCYYGGVDTTPLFVMLAGAYEKQTGDRSVVDAIWDNLLAAIG
                     WVEGRMRRSPTGFLDYARGQKSGLANQGWKDSQD"
BASE COUNT          546 a          948 c          905 g          546 t
ORIGIN      
        1 tccggcaccc gatcgaacac gacgcgcacc ggcaggccgt cgtcgcgcag cacctcgatc
       61 cggttgccga tctgcagcac cgtgccgccg ttcacggcca gcaccttcgc ctgctcgcgc
      121 gtctcctggc cggtcgccgg attggtccgc accaaggtga tcgtctcgcc caccgccttc
      181 tgcatcaggg cgcttggcga gagcaggtcg aagtcgaaat tctgctcgac gatcgacaga
      241 tcactgcagg tcagcgaaac ggtctcggag cggatcttcg ccgcgacgtc ctgaaactcg
      301 atgcgctgcc gcccggcagc gatctgaatg tcgcgtgtat cctgaatcag cgccagatca
      361 ttcgcataga tggtcacgga cagcgaccgg tctgccgccc ccgcgcagaa cgggccactg
      421 agcacgagtg ccagcgtcat gggtaagaat cgcgcgcgca ttacggctcc cttatgtaaa
      481 tgccgtgcgc cggtcgggct gacggtccag ctgcgcatca tggcgattcc ggcagtcagt
      541 tggcaactcc cgcgccgcgg cagccagccc ctggggcatg aacattgctc atcaatggca
      601 tgaagatcgc ccagattgcg ccgctcgatg agtcagtgcc accgcagttg tatggaggga
      661 ccgagcgtgt cgtcgcgcat ctgtgcgatg ccctggtgga ccgcgggcat gaggtgaccc
      721 tgtttgctgc tgccggcgcg cgaaccaggg cccaccaggt cagcgcgcgc gaccagccga
      781 tccggctcga caaaagcgag ctgaaatcgg agttgggggc acatctgagc atgcttcatg
      841 acgtgcgcga gcgcgccggc cagttcgaca tcctgcactt ccacactgag atgctccatt
      901 tcccgatgtt tgagcagctc gcccacaaga ccgtcacgac actgcacggc cgcctggacg
      961 tcaaggacct gcccggagtc tactcacgct ggccacagtt cgggctcgtg tcgatctcca
     1021 acgatcagcg ccggccgctg cctcacgccc attggcttgc tactgtgcca cacggcctcg
     1081 agctcgatct gtatcgtttt caagagcgct cccgccccgg ctatctcgcc ttcctcggcc
     1141 ggatctcccc cgaaaagggc gccgatgcgg ccatccgcat tgcaagcaag gccggtctgc
     1201 cgctgaagat cgcggcgaag gttgacgatg ctgacgcagt gtatttcgat agcgagatca
     1261 agcccctgct ggagaaccag gctgtcgagt tcgtgggtga gatcggagat gagcagaaat
     1321 cacagttcct gggcgatgcg ctcgcactgt tgtttccggt cgattggccg gaacctttcg
     1381 gactcgtcat gatcgaagcg atggcgtgcg gtacgcccac catcgcgtgg cagcggggct
     1441 cggtacccga ggtcattgac gaaggcgtga ccggactgat cgtcaactcc atcgaggaag
     1501 cagtcgcggc cattccgcgc gtccgccagc tcgatcgaca gcggatccgc gccacgttcg
     1561 agcgccgctt cggcgccgag acgatggcca atgcctactt ggacgtctac aaccgcttgc
     1621 tggcctcgcg gcccaccgca gcaccgtaaa ctggaaagcg attcatcatg agttccgcga
     1681 acgaagagtc cgctgagcgt cagggtctgc tgccgcgctt taccctcaag gatgcggata
     1741 tgtttcttct ttccgatgcc ctgggcgata tccaaagcag ccgcgacggc ctgttttcaa
     1801 acgacacgcg catgttatcc cgctacgagc tgcagatcgc cgagcgccgt ccggcactgc
     1861 tcggcgccgc gctctcgcag gacaacacaa tgttgacctc gcacctgaca aacctgccgc
     1921 tgccgatact cggtgaagga gtgattccca ggggggtgat ccacatcgag cgctcccgtc
     1981 tgctgtggca gggcagcctg cacgagcggc tgaagctcac caatttcggc aatctggtgg
     2041 ctcaagtgcc gctgcgattc tgctttgacg ccgattttgc cgacatcttc gagattcagg
     2101 ggaccgttcg gcagcgcagc ggcgcgcgcc agccacccga aatcgacggg cgaactgtgc
     2161 gactgggcta ccgcggcctc gatgagcgcg agcgggtcac ctatctggct ttttcacggg
     2221 tcccgcaggt tctggaaaca cacgaagcca gattcatgtt tgagctgcat aaccgtgagc
     2281 ccgtcgagtt gttcgtcgag atcagcactg cggcgcaacc ggcacccgcg gcggagaggt
     2341 ttgcggctgc cgccgaggga gcggcggcta aaatgagtca acgtgttgca gctgcagcga
     2401 cgctccgtac gtccggacga ctgttcgatg aatggataaa caaatcacga tccgatctgg
     2461 cgctgctcac cacggatctg gcgaccggac cctaccctta cgcgggcatc ccctggtttg
     2521 ccactcaatt tggccgtgat gccatcatca cggcattaca gacactctgg acgcaacccc
     2581 gcctggcagc gggcgtgctg cgcttcctgg cttccacgca agctcgtgaa acctccgcat
     2641 tccgcgactc cgagcctggc aagatcatgc acgagagccg ccggggtgaa atggcggccc
     2701 tgggagaagt accgttcggc tgttactacg gcggtgttga taccacgcct ctgttcgtga
     2761 tgctggccgg cgcctatgag aagcaaacag gcgaccggag cgtcgtggac gcgatctggg
     2821 acaatctgct ggcggccatc ggctgggtcg agggccggat gcggcgcagt ccgaccgggt
     2881 ttctggacta cgcccgcggt cagaaatccg ggctggccaa ccagggatgg aaggacagtc
     2941 aggac
//
LOCUS       DASRHN010000182        10142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_731690, whole genome shotgun sequence.
ACCESSION   DASRHN010000182 DASRHN010000000
VERSION     DASRHN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731690"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFB37_07025"
     CDS             <1..416
                     /locus_tag="VFB37_07025"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZO22243.1"
                     /translation="AADAARTTCQAPDVLEDCRILARALHAALSGQPKERILAGTKFP
                     SVAAAQRTDTSSGVLQAAFWAFSSTDSFRDAVLRAANLGGNSDVVAAVCGQLAGAFYG
                     QAAIPAPWSNSLMHKDLIESYGDRLLTQCLLSLGS"
     gene            610..1587
                     /locus_tag="VFB37_07030"
     CDS             610..1587
                     /locus_tag="VFB37_07030"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HZO22244.1"
                     /translation="MLSLRFCDLKLSVEGSRLRHRVKRLYAELESRGISVRPHVWLSE
                     EWFSPDGVPGIAVPFYLAHPRLERLERRIMREVEGGNGRQLMRILRHEAGHAVDNAYR
                     LRRRKRWREIFGPASLQYPHRYKARPGSRRYVHHLGEWYAQAHPTEDFAETFAVWLKP
                     NSDWRRSYAQWPAIHKLSAVEELMASVRGRRAPVRNRTRIEPIDRNTRTLGQYYRRKL
                     ARNHQIRTVITDELLQRVFASERPKRPTVRAAALLRACRAQSVSSVARVLGMERYSVD
                     QILGMLIERAESLSLFVRGSRRDAIRSSRWMLERIARLYSQSETPHLPL"
     gene            1584..2618
                     /locus_tag="VFB37_07035"
     CDS             1584..2618
                     /locus_tag="VFB37_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22245.1"
                     /translation="MRKLRTLVVVHASLVPPENLEGHTNKEIDEWRTEYDVTTTLRKC
                     GHDVRVTGVLDSLTELRSAIADWKPDVVFNLLEEFNGIVTYDQHVVAFLELMRQPYTG
                     CNPRGLLLSRDKSLCKQLLAFHRIPTPQFAVFRRGARVRVPRKLRFPLFVKSTVEDAS
                     LGIAQASVVEDQAKLKERIEFVHEQIKSDALVEEYIEGRELYVGVLGNDRLSRLPVWE
                     MVFGSMPDTQAAIATRKVKWDKSYQSKYGITTRAAEDLPAAVLASLDKLSRRIYRTLG
                     LSGYARMDFRVRADGQVYVLEANANPNLEAAEDFAESARAAGVSYDELLEKIMALGSS
                     YRAEWRAFYG"
     gene            2611..3315
                     /locus_tag="VFB37_07040"
     CDS             2611..3315
                     /locus_tag="VFB37_07040"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HZO22246.1"
                     /translation="MDERIRWRGWLARGLFLLACLSVAAWGNKGPKPEEELQELLQWF
                     PGHYDNNAQYQADVRQNVQPPHDALAVDIVPIDAPMIGDHVFYLQESAADDPRRVTAQ
                     RIIVFGVVKKDIVETIWSLAEPRRWRDGQRNPDLFKSLMTQDVHSSKGCSLRWHRSEG
                     KFVGADEPKTCHGASGGTGLAHIEYRAELGPEEYALAELAFDNSGHLVRGRRDEPFYR
                     FRKQPRDPEDASSGGE"
     gene            complement(3348..5477)
                     /locus_tag="VFB37_07045"
     CDS             complement(3348..5477)
                     /locus_tag="VFB37_07045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO22247.1"
                     /translation="MFACRARSGCHPVLVSLSALLFTGVAPFSPAAQPNTHTAAPDAA
                     ASELKPFTVEDLVRLKRLTDPQLSPDGHYIAFVLRETDIEANRGRTGIWLLDLTQKGA
                     TPRRLTQSEANDSSPRWAPDSRTIYFLSARSGSSQVWRLTLSGGDAERVTDYPLDVDT
                     LKVSPTGTRIAIAMGVLPDCEDLRCTKDKLAARGKNKATGQIYNRLFVRHWDTWDNGT
                     RSHLFSARINADGKADTPVDLSRSLDADVPSKPFGGDEDFAFSPDGNRLVFSARIAAR
                     TEPWSTNFDLFEVPVDRSTQPRNLTENNPAWDAQPVFLRNGDLAWLAQDHPGQESDRF
                     HIMLKEARTGEVKPLTQAWDRSVIRLGVTPDGRRLLATTEDLGQTSLFSIDVARGTPH
                     RIVGTGEVSDYAASNSSVVFGWANLGAPADLYQVEIGGGTPQRLTQVNAELMQRRRLS
                     EFEQYSFKGWNDETVYGYVMKPYGFTSGQRYPIAFLVHGGPQGSMQNIWHYRWNAQAF
                     AGAGYAVVMIDFHGSNGYGQAFTDSISRDWGGKPLEDLQKGLAAAIGRYPWLDGERAC
                     ALGGSYGGFMMNWIEGNWSDRFRCIVNHDGVFDQRMMYYSTDELWFMEWENGGPQYEN
                     PQGYERFNPVNSVARWHTPMLVVHGEQDFRIPYTQGIAAFTALQRRGIESRLLLFPDE
                     NHWVLKPANSVMWYHQVLGWLDEHLKK"
     gene            complement(5617..6270)
                     /locus_tag="VFB37_07050"
     CDS             complement(5617..6270)
                     /locus_tag="VFB37_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZO22248.1"
                     /translation="MSELHLITGNKNYSSWSLRAWICLKHLGIPFRETLVRLDQAQFK
                     EQVARYGPTGRVPVLLDGELSIWDSLAICEYAAEVAGRGWPADRAARAIARSVCAEMH
                     SGFTSLRAQWPMNARARNRHTVLTAELQADLGRIDAIWSDCRRRFGRDGGAWLFGRYT
                     VADAMYAPVVLRFNTYGAPLSETAQAYVATALEDAALQEWLRDAQNEPWTIPHDEVG"
     gene            6348..7037
                     /locus_tag="VFB37_07055"
     CDS             6348..7037
                     /locus_tag="VFB37_07055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZO22249.1"
                     /translation="MHDSIRAVAFDLDNTLWDVEPVIARAEARWLAWMREHCPRIPER
                     LSLEDMRIARQQLAAREPHNAHDFTYLRITSLANQARECGYEPAIAERAFEIFIAARN
                     ELDLYEDVRPGLDRLRARFALATLSNGNADVARIGLGEYFAVSLNARGIGAAKPDPRC
                     FEHLAQVLQLQPHEVLYVGDDPLLDVAAARASGLRTAWMNRTGLPWPPTVTPADVDVV
                     DCIALAERLSA"
     gene            complement(7075..8418)
                     /locus_tag="VFB37_07060"
     CDS             complement(7075..8418)
                     /locus_tag="VFB37_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO22250.1"
                     /translation="MPGTEQSSRTFLGFSGYHWLVIAAAWAGWGFDVFDALLFNFVAP
                     NCVPALLHLPPGTPAAQQATVFWTGALSSILLVSWAAGGVLFGWVGDRIGRQRALFVT
                     IAVYALGTALCAAATNIWMLVAFRTLASLGIGGEWGIGAALVAEAVPQSKRVEAGVIL
                     QTSSPLGLVLATGVNYLIAGVWLADSPQSSWRYVFLAGLAPVLLAVFIRMFVHESAQW
                     SAAAARTNPPSPRELFNAGNFRLTLSGVTVAVTAILTWWACNAFIPLLGSTLAGEHAA
                     QLGLSGEAARLLAETWKARASNAFNVGGLIGAFAAIALARPLGRRPMFVIYFLFSAGV
                     LFATFGLDLAPATRLAMLFFVGAGVYGVFGTFPFYLPELFPARIRATGAGFCYNIGRV
                     LAAVGPVFIGLLSSRAGGSSAVINHALLWVGLIPLAAALSARYIIVETRGLPLHA"
     gene            8631..9260
                     /locus_tag="VFB37_07065"
     CDS             8631..9260
                     /locus_tag="VFB37_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22251.1"
                     /translation="MTDGRRSTRVLPVANLREFFKDELHGALEKQQLEVEDQTEHYVV
                     NLLTAFARSEALYEPTPEGNRVKPLVVMLSEALEAPSAEDRNRGLQRLGDVSLFIAGF
                     FSQSFARKLIDIDYHIAMGGRAYGTLAEALCRGRGRVLGNVFAELARKFQPLVDALNE
                     VSETSYNHSDKDILRLYEIWLKTGSQRSYNLLRRLGVEPTTAGGTAFAH"
     gene            9265..9927
                     /locus_tag="VFB37_07070"
     CDS             9265..9927
                     /locus_tag="VFB37_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22252.1"
                     /translation="MILKQLQDLIGDIYDVSIAHDVYDFLVTDRRRLPAAARANTATD
                     EELIVAQPVEGSEGEIAMSLYLDPELLERLKRADPMVRLHDGNVADYWTALEGVSHFL
                     YLAWNAGHDKPVSLLELEMQAEVDKYVASYWLMRRQFPDRFPAELFRLLFERTRIDPR
                     LAAGREDLYREASRYAQKFCRRLEGSLKRTRGAPAEVLAELRRFYRFTNMRKVAHIER
                     TA"
     gene            complement(9924..>10142)
                     /locus_tag="VFB37_07075"
     CDS             complement(9924..>10142)
                     /locus_tag="VFB37_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22253.1"
                     /translation="RQPAKPSRPGLNTGSLSIHTEDTFNPYARVPESDQKAGGRPKDL
                     RRLSAWIKLMRQLEESKRKGQDEEDEPT"
BASE COUNT         1818 a         3285 c         3303 g         1736 t
ORIGIN      
        1 acgcggccga tgccgctcgc acgacctgcc aggcaccgga cgtgctcgag gactgccgga
       61 tcctggctcg ggctttgcac gcggccctct ccgggcagcc caaggagcgg atactcgctg
      121 gcacaaaatt cccctccgtc gctgccgccc agcgcaccga cacgtcctcg ggcgtcctgc
      181 aggccgcctt ctgggcgttc tcctcgaccg acagcttccg ggatgcggtg ctgcgggccg
      241 ccaatctcgg cggaaactcg gacgtcgtgg cggcggtttg cggccagctg gccggcgctt
      301 tctacggcca ggctgccatt cccgcccctt ggagcaacag cctgatgcac aaggacttga
      361 tcgagagcta cggcgatcgc ctgctcaccc agtgcctgct ctcgctcgga agttgaaatc
      421 tgcaccgatc aaccgcagac accgcccggg gactttggta atttctccgc tcgctcgcac
      481 cccagacgga accctctcga gagaccgatg gcccaggccg cccgtaattg cgccgcctcc
      541 gccggccgcg ccgcacacgc tcctcgcgca cgccccgcac atgcgtggac gcgactaagc
      601 gacgaagcgt tgctgtccct gcgtttctgc gatctgaagc tctccgtcga gggctcgcgg
      661 ctccggcatc gcgtcaaacg cctgtatgca gagctcgaga gccgcggcat ttccgtgcgt
      721 ccgcacgtgt ggctgtccga ggagtggttc tcgcccgatg gcgtgccggg catcgccgtc
      781 ccgttctatc tcgctcatcc gcgcctcgag cgcctcgagc gccgcatcat gcgcgaggtg
      841 gaagggggca atggccgtca gctcatgcga atacttcggc acgaggccgg tcatgccgtg
      901 gacaacgcgt accgcctgcg ccggcgcaag cgctggcgcg agatattcgg cccggcatca
      961 ttgcagtatc cacatcgata caaggcgcgc cccggaagcc gccgctacgt gcatcatctg
     1021 ggcgagtggt atgcgcaggc gcatcccacg gaagacttcg ccgagacgtt cgcagtgtgg
     1081 ctgaagccga attccgactg gcggcgcagc tacgcgcaat ggccggcgat tcacaagctg
     1141 agcgcagtcg aagagctcat ggcgagcgtg cgcgggcggc gggcgccggt ccgtaaccgc
     1201 actcgcatcg agccgatcga ccgcaacacg cggacgctgg ggcagtatta ccggcgcaag
     1261 cttgcgcgta atcatcagat tcgcacggtg atcaccgatg agctgctgca gcgggtattt
     1321 gcctcggagc gtccgaagcg acccacggtg cgtgccgccg cgctgctgcg cgcctgcaga
     1381 gcccagagcg tctcgagcgt ggcgcgcgtg ctcggcatgg agcgctacag tgttgaccag
     1441 atcctgggca tgctcatcga gcgcgccgag tcgctgagcc tgttcgtgcg gggaagccgg
     1501 cgggatgcaa tccgttcctc acgctggatg ctggagcgga ttgcccggct gtactcgcaa
     1561 agcgagacgc cacaccttcc cctatgagga aactgcgcac gctcgtggtc gtgcatgcga
     1621 gcttggtgcc cccggaaaat ctcgaaggtc acacgaacaa ggaaatcgac gagtggcgta
     1681 ccgaatacga cgtgacgacc actctgcgca agtgcgggca cgatgtgcgc gtcacggggg
     1741 ttctggacag cctgactgag ctgcgcagcg cgatcgccga ctggaagccg gacgtcgtct
     1801 tcaacctgct cgaggaattc aacggcatcg tcacctacga ccagcacgtc gtggccttcc
     1861 tcgagctgat gcgccagccc tacaccggct gcaacccgcg cgggctgctg ctctcgcggg
     1921 acaagtcgct gtgcaagcaa ctgctcgcct tccatcgcat tccaactccg cagttcgcgg
     1981 tattccggcg tggcgcccgc gtgcgcgtgc cacgcaagtt gcgctttcca ctgttcgtga
     2041 agtccactgt cgaggatgcc tcgctcggga tcgcccaggc ctcggtggtg gaggatcagg
     2101 cgaagctcaa ggagcgcatc gagttcgtgc atgagcagat caaatcggat gcgctggtgg
     2161 aggagtacat cgaaggccgc gagctctatg tgggggtgct ggggaatgat cgtctgagcc
     2221 ggttacctgt ctgggagatg gttttcggct ccatgccgga cacccaggcg gcgatcgcga
     2281 cgcgcaaggt caagtgggac aagagctacc agagcaagta cggcatcacg acccgtgcgg
     2341 cggaggatct gcctgcagca gtgctggcga gcctcgacaa gctttcccgg cgcatctacc
     2401 gcacgctcgg cctgtcgggt tacgcgcgca tggatttccg ggtgcgcgcc gatggccagg
     2461 tgtatgtgct cgaggccaat gccaatccca acctggaggc cgccgaggac ttcgcggagt
     2521 ccgcccgcgc ggccggagtg agttacgatg agctcctgga gaagatcatg gcgctcggct
     2581 cgtcgtaccg tgccgagtgg cgggcatttt atggatgagc ggatacgctg gagggggtgg
     2641 cttgcgcgcg ggctcttttt gctcgcgtgc ctcagtgtcg cggcgtgggg caacaagggt
     2701 cccaagcccg aagaggagct gcaggagctg ctgcagtggt tcccgggcca ctacgacaac
     2761 aacgcgcagt accaggccga cgtgcgccag aatgtacagc ctccgcacga tgcgctggcg
     2821 gtcgacatcg tgccgatcga tgcgccgatg atcggcgatc acgtgttcta tctgcaggag
     2881 tcggcggccg atgatccacg gcgcgtcacg gcgcagcgca tcatcgtgtt cggtgtcgtc
     2941 aagaaggaca tcgtcgagac gatctggagc ctcgccgagc cgcgccgctg gcgcgatggg
     3001 caacgcaatc ccgacctctt caagagcctc atgacgcagg atgtgcattc gagcaagggg
     3061 tgcagcctgc gctggcaccg atccgagggg aaattcgtcg gcgcggatga gcccaagacc
     3121 tgtcacggtg cctccggcgg tacgggactc gcacacatcg agtatcgcgc cgagctcggc
     3181 ccggaagagt acgcgctcgc agagctggcc ttcgataact ctggccacct ggtgcgcggg
     3241 cgacgcgacg agccgttcta ccgcttccgc aagcagccca gagacccgga agacgcatcg
     3301 agcggcggcg agtagctcgc gcggcagcgc gtagcgcctg cggcgcgcta tttcttgagg
     3361 tgctcgtcga gccagcccag cacctggtgg taccacatca cgctgttggc cggcttgagc
     3421 acccaatggt tctcatccgg aaacagcagc agccggctct cgatcccccg gcgctgcagc
     3481 gccgtgaagg ccgcgattcc ctgggtgtac ggaatgcgaa agtcctgctc gccatgcacg
     3541 accagcatgg gcgtgtgcca gcgtgccacg gagttcaccg ggttgaatcg ctcatagccc
     3601 tgcggattct cgtactgcgg tccgccgttc tcccactcca tgaaccacag ctcatcggtc
     3661 gagtagtaca tcatccgctg atcgaacacg ccgtcgtggt tgacgatgca gcggaagcgg
     3721 tctgaccagt tgccctcgat ccagttcatc atgaaaccgc cgtacgagcc gccgagagca
     3781 cacgcgcgct cgccatcgag ccacggataa cgcccgatcg cagccgcgag tcccttctgc
     3841 agatcctcga gcggcttgcc gccccaatcg cggctgatcg agtcggtgaa ggcctggcca
     3901 taaccgttcg agccatggaa atcgatcatc acgactgcat aacctgcgcc ggcgaaggcc
     3961 tgcgcgttcc agcgatagtg ccagatgttc tgcatgcttc cctgcggtcc gccatgcacg
     4021 agaaatgcga ttggatagcg ctgtccggat gtaaagccat acggcttcat cacgtagccg
     4081 tagaccgtct catcgttcca acctttgaag ctgtactgct cgaactcgct caggcgccgc
     4141 cgctgcatca gctcggcatt cacctgtgtc agccgctgcg gtgtcccgcc gccgatctcg
     4201 acctgatata gatcggctgg cgctccgagg ttggcccagc cgaacactac ggagctgttg
     4261 ctcgcggcgt agtcgctgac ctcacccgtg ccgacgattc gatggggcgt tccacgcgcg
     4321 acgtcgatcg agaacaggct cgtctggccc agatcctccg tggtcgcgag caagcgccgc
     4381 ccatcgggcg tgaccccgag acgaatcacg gagcgatccc aggcctgcgt gagcggcttc
     4441 acctcgcccg ttcgcgcctc cttgagcatg atgtgaaagc gatccgattc ttgccccgga
     4501 tggtcctgcg ccagccaagc caagtcgccg ttgcgcagga agaccggctg cgcatcccag
     4561 gcgggattgt tctccgtcag attgcgcggc tgggtcgagc gatccaccgg cacctcgaac
     4621 agatcgaagt tggtggacca gggctccgtg cgagcggcga tccgcgccga gaacaccagg
     4681 cggttgccgt ccggactgaa agcgaagtcc tcatcgccgc cgaagggctt cgagggcaca
     4741 tccgcatcga gcgatctgga gagatccacg ggcgtgtcgg ccttgccatc cgcattgatg
     4801 cgagccgaga acaggtggga gcgggtgccg ttgtcccagg tatcccagtg gcggacaaac
     4861 agtcggttgt agatctgtcc ggtggctttg tttttgcccc gcgcggcaag cttgtccttc
     4921 gtgcaacgca ggtcctcgca atccggcagc acgcccatcg cgatcgcgat gcgcgttccc
     4981 gtcggtgaga ccttcagggt atccacatcg agcgggtagt cggtgacgcg ctcggcgtcc
     5041 cctcctgaga gggtcaatcg ccacacctgc gagctgcccg agcgggcgga cagaaaatag
     5101 atcgtccggc tgtccggcgc ccagcgcggg ctggagtcgt tggcctcact ttgcgtgagg
     5161 cgccgcggcg tcgcaccctt ctgcgtcaga tcgagcagcc agatgccggt ccggccccga
     5221 ttggcctcga tgtcggtctc gcgcagcacg aaggcgatgt agtgtccgtc gggggagagc
     5281 tgcgggtcgg tcaggcgctt gagcctcacg aggtcttcga ccgtgaaggg cttcagctcg
     5341 gaggcagctg catccggcgc ggccgtgtgt gtgttgggct gagcggcggg cgagaaaggg
     5401 gccacgccgg tgaacagcag cgcgctgagg ctgacaagga ccggatggca gcccgagcgg
     5461 gctctgcagg caaacataga cggctcccgg gaaaaagagg cggccattgt acctgcgcct
     5521 gtcagggacg acgtgtcggt acgggactca tccgcgggct acactgcgcg cagcccctgg
     5581 caaagtcggc cgcccgccaa gctcctcggg caaggctcag ccgacctcat cgtggggaat
     5641 ggtccagggc tcgttctgcg cgtcccgcag ccattcctgg agtgccgcat cctccagtgc
     5701 cgttgcgaca tacgcttgcg cagtttcgga gagcggcgcg ccgtaagtgt tgaaccggag
     5761 cacgaccggt gcgtacatcg catccgccac cgtgtatcgc ccgaacagcc aggcaccgcc
     5821 atcgcggccg aaccggcggc ggcaatcgct ccagatcgca tcgatgcgcc cgaggtcggc
     5881 ctgcagttct gcagtcagga ccgtgtgccg atttcgggcg cgcgcgttca ttggccactg
     5941 ggcgcgcaga ctggtgaaac ccgaatgcat ctcggcgcat accgagcgcg ctatggccct
     6001 cgccgcacga tccgccggcc agccccgacc agcaacttcg gccgcgtact cgcagatggc
     6061 aagggagtcc cagatcgaaa gctcaccgtc gagcaggaca ggcacgcgcc ccgtcggccc
     6121 gtagcgcgcc acctgctcct tgaactgcgc ctggtcgagc ctcaccagcg tttcgcggaa
     6181 cgggatgccc agatgcttca ggcagatcca cgcgcgcagc gaccaggatg agtaattctt
     6241 gttgcccgtg atcagatgca gttccgacat gcagctccca tgcccagcgg ctcgacggcg
     6301 attgcgtgaa acaggctaga atctgccgcc gagccttttg caaagccatg cacgacagta
     6361 tccgcgcggt tgccttcgat ctggataata ccctctggga tgtcgagcct gtgattgcgc
     6421 gcgcggaggc gcgctggctg gcgtggatgc gtgaacactg tccgcgcatc cccgagcgtt
     6481 tgtcgctcga ggacatgcgc atcgcgcgcc agcagcttgc ggcacgcgag ccgcacaacg
     6541 cgcacgactt cacctatttg cggatcacct cgctggcgaa ccaggcgcgc gaatgcggct
     6601 atgagcccgc gatcgcagag cgggcgttcg agattttcat cgccgcccgc aacgagctcg
     6661 atctgtacga ggacgtccgc cccgggctcg atcggctgcg ggcgcgtttt gcgctcgcta
     6721 cgctcagcaa cggcaatgcg gatgtggcac ggatcgggct cggcgaatac ttcgcggtct
     6781 cattgaacgc gcgcggcatc ggcgctgcca agcccgatcc gcgctgcttc gaacacctgg
     6841 cccaggttct gcaattgcaa ccgcacgaag tgctctacgt gggcgatgat cctctgctcg
     6901 atgtggccgc ggcgcgcgcc agtggcctgc gcaccgcctg gatgaatcgc accggcctgc
     6961 cttggccgcc aaccgtcacc cctgccgatg tcgatgtcgt cgattgcatc gccctggcgg
     7021 agcggctgag cgcgtaacga cttgcgcagc ggaaccgaac agacgaccag ctcgctaggc
     7081 atgcagcggt aacccgcgcg tctcgacgat gatatagcgc gcgctgaggg ctgcagcgag
     7141 cgggatcagg cccacccaga gcagcgcatg attgatgacg gcgctcgagc cgcccgcgcg
     7201 cgaggagagg aggccgatga agaccggccc aactgctgcg agaacccgcc cgatgttgta
     7261 gcagaagccc gcaccggtgg cgcggatgcg tgccggaaac agctccggaa ggtagaaggg
     7321 gaaagtaccg aacacgccgt agacccctgc acccacgaag aacagcatgg ccaggcgagt
     7381 tgcgggtgcg aggtccagtc cgaaggtcgc gaagagcaca ccggcggaaa agaggaagta
     7441 gatgacgaac attggtcggc ggccgagcgg ccgggccagt gcgatggctg cgaaggcgcc
     7501 gatgagaccg cccacgttga aggcattgga ggcgcgcgct ttccaggttt ccgcaagcaa
     7561 tcgggcagcc tcaccggata gccccagctg ggctgcatgc tcgcccgcca gggtgctgcc
     7621 cagaagtgga atgaaggcgt tgcacgccca ccaggtgaga atcgcggtga ccgctaccgt
     7681 cacgccgctg agggtcagcc ggaagttgcc ggcattgaac aactcacggg gcgagggcgg
     7741 attggtccgc gccgcagccg cggaccactg cgcgctctcg tgcacgaaca tacggatgaa
     7801 tacggcgagc agcacgggtg cgagcccggc cagaaagaca taacgccacg agctctgcgg
     7861 gctgtccgcc agccagacgc ctgcgatcag gtagttgaca cccgtcgcca acacgagccc
     7921 gagcggggag gaggtttgca ggatcactcc ggcctcgacg cgcttgctct gcgggactgc
     7981 ctcggcgacc agagccgcac cgatgcccca ctctccgccg atgcccagac tcgccagggt
     8041 tcgaaaagca accagcatcc agatgttggt ggctgcggcg catagtgcgg ttcccagggc
     8101 gtagaccgcg atggtcacga agagcgcgcg ctggcgtccg atacggtcgc ccacccagcc
     8161 gaacaatacg ccgccggctg cccagctcac gaggagaatg gaggataggg cgcccgtcca
     8221 aaacacggtc gcctgctgcg ccgctggcgt ccccgggggc agatgcaaca gcgcggggac
     8281 acagttcggg gcgacgaagt tgaacagcag ggcatcgaaa acatcgaagc cccaacccgc
     8341 ccaggcggcg gcgatcacga gccagtgata gccggaaaag ccgagaaaag tccggctcga
     8401 ctgctctgtt cccggcaccc cgttaccccc tcggcggctg ttagcagtgt atcggtttcg
     8461 ctgccagagg tgacaggtat ccgtgtccct gggaaacccc ctgccgggcc aagcgttaca
     8521 tactgattgt agtagcgcgt cacagtatcc cccactacgg cggagtgaac tttccgggcc
     8581 ggctgccgtt ctctaatgta gagagagtga gcgtaaggag gcccgatccc atgaccgatg
     8641 gtcgccgctc aacgcgcgta ctgcccgttg cgaatctgcg cgagttcttc aaggacgagc
     8701 tgcatggcgc gctcgagaaa cagcagctcg aagtcgagga tcagaccgag cactacgtgg
     8761 tcaacctgtt gaccgccttc gcccgctccg aggcacttta tgagcccact cccgagggca
     8821 atcgcgtcaa gcctctcgtg gtcatgctct cggaagcgct cgaggcgccc tccgccgagg
     8881 acaggaatcg cggtttgcag cggctgggcg atgtgtcgtt gttcatcgcc gggtttttct
     8941 cgcagagctt tgcgcgcaag ctcatcgaca tcgactatca cattgccatg ggcggacggg
     9001 cttatgggac gctggccgag gcgctctgcc gcgggagggg ccgggttctc ggcaacgtct
     9061 tcgccgagct cgcccgcaag tttcagccgc tggtggatgc gctcaacgag gtcagcgaga
     9121 cctcctacaa ccatagcgac aaggacattc tgcggttgta cgagatctgg ctgaagaccg
     9181 gcagccagcg cagttacaac ctgctgcgcc gcctgggcgt cgagccgacc accgccggcg
     9241 gcacggcgtt cgcgcactga ttgcatgatc ctgaagcagc tccaggatct catcggcgac
     9301 atttacgacg tgagcattgc tcacgatgtc tatgacttcc tggtgacgga tcgacggcgc
     9361 ctgccggcgg cagctcgagc caataccgcg accgacgagg agctcatcgt cgcgcagccg
     9421 gtggagggca gcgagggcga gattgccatg tcgctctacc tggatcccga gttgctggag
     9481 cggctgaagc gcgccgatcc catggtccgc ctgcatgatg gcaacgtggc cgattactgg
     9541 actgcgctcg aaggcgtcag tcactttctg tacctggcct ggaacgcagg acacgacaag
     9601 ccggtgtcgc tgctcgagct ggaaatgcag gccgaagtgg acaagtacgt ggcgagctac
     9661 tggctgatgc ggcggcagtt tccggatcgc tttccggccg agctcttccg cctgctgttc
     9721 gagcgcacgc gaattgatcc gcgcctcgcc gccggccgcg aggatcttta tcgcgaggcg
     9781 agccgctatg cgcagaagtt ctgccgccgg ctggaggggt cattgaagag aacgcgaggc
     9841 gcgccggcgg aagtgctggc cgagctgcgg cgcttctatc gattcacgaa catgcgcaag
     9901 gtcgcgcaca tcgagcgcac cgcttaggtg ggctcgtcct cttcgtcctg acccttgcgc
     9961 ttgctctctt cgagctgccg catcagcttg atccaggccg acaggcggcg aagatccttc
    10021 ggccgtccac cagccttctg atccgactcc gggacgcgcg cgtagggatt gaaagtatct
    10081 tcggtgtgga tcgacagcga gccggtattc aggcccgggc ggctcggctt ggcgggctgg
    10141 cg
//
LOCUS       DASRHN010000183         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1218363, whole genome shotgun sequence.
ACCESSION   DASRHN010000183 DASRHN010000000
VERSION     DASRHN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218363"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFB37_07080"
     CDS             <1..801
                     /locus_tag="VFB37_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HZO22254.1"
                     /translation="AQMLAIARELNNADTAFILSPESGDYNVRARFFTPRTEAGFVGH
                     ATVAAHYVLSRRHDAPRHLRQKSKAGMVDIEIRGTGEDRSIAVRQTTPPLGRELNDRE
                     RLAVLDALALATDDLDIRCPLRIVGSSSTRLMVGVRGQDPLRHLKPDMTRLTTLSAQI
                     GAAGYFVFCLTQGAGDHLTESRMFCPALGIAEDPVSGNAHGLLGVYLAHCGLLSRGER
                     RTMFTGIQGHSVHRPGRVDVELEFRDGSVEAVWIIGQAVSIFETEIAL"
     gene            801..1121
                     /locus_tag="VFB37_07085"
     CDS             801..1121
                     /locus_tag="VFB37_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZO22255.1"
                     /translation="MTEPVFVPILALTEIPQGRMQTRVLEGREIVICHTREGVFALDN
                     ICTHAHARMCEGRLRSMRLICPLHGASFDIRDGRVLGSPAIIPLRTYPVRVVNGTIEI
                     SLTV"
     gene            complement(1111..>2237)
                     /locus_tag="VFB37_07090"
     CDS             complement(1111..>2237)
                     /locus_tag="VFB37_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO22256.1"
                     /translation="LIPLVPYMAERFGAAPAVITALFGVYSLCQLIAAPLWGRLSDRF
                     GRRPILMSSLAGACASYVILGLADGLGWLFASRILGGFMAGNIAAAFAYASDVSKPEQ
                     RAASLGMVGAAIGIGFTLGPAIGGLLAGNDLRAASFVRPAAVSAGLSLFAIALVAFLL
                     PESNTAERRGEHIARERAGPLRLLIERPALRWLATAALLVTFSQAVLESIFAIWALNK
                     FGFGPRTVGLLMFCLACLAVLMQGGGVRLLVPRLGEVKLASVGVLAYVAGLVTVAAAP
                     NLALTGFGLALCGVGVGSFNPSASALASKQAQAHDRGSVMGTYQSSSSLARVIGPFIS
                     GPVYAALGPSAPFLVGACVTLPAIWFVWLAHLRAQEPVRQ"
BASE COUNT          439 a          730 c          726 g          342 t
ORIGIN      
        1 gcgcagatgc tcgcgatcgc gcgcgagctc aacaatgccg atacggcttt catcctctcc
       61 cccgagagcg gggactacaa cgtgcgggcg cggttcttca cgcctcgcac cgaggcgggt
      121 ttcgtcggac acgcaacggt agcagcgcac tatgtgctct cgcgccgaca tgacgcgccc
      181 cgccacctgc ggcagaagtc aaaggcaggg atggtggaca tcgagatccg cgggaccggc
      241 gaggatcgca gcattgctgt ccgccagacc accccgcccc tgggtagaga gctgaacgat
      301 cgcgagcgtc tcgcggtcct cgatgctttg gcgctggcca cagatgacct ggacatccgc
      361 tgtccgctgc ggattgtcgg ctcgagcagc acgcggttga tggtgggcgt acgtgggcag
      421 gatcctctgc gacatctgaa gccggacatg acccgcctca ccactctatc ggcacaaata
      481 ggcgcggcgg gatacttcgt tttctgcctc acacagggtg cgggggatca tctcacggag
      541 tcccgcatgt tctgcccggc gctcgggatt gctgaggatc cggtcagcgg caacgcacat
      601 ggtctgctag gcgtatatct cgcgcattgc ggtctgctct ctcgaggcga gcggcgcacg
      661 atgttcaccg gcatccaggg ccattcagtg caccggcccg gtcgggtcga cgtggagttg
      721 gaattcaggg acggcagcgt ggaagcggtc tggatcattg gccaggcggt ctcgatcttc
      781 gagaccgaga tcgcgctctg atgacggaac ccgtattcgt ccccatcctg gctctcaccg
      841 agattcccca aggaagaatg cagacacgag tgctggaagg ccgcgagatc gtgatctgtc
      901 acacgcgcga aggcgtattc gctctcgaca acatctgcac tcacgcgcac gctcggatgt
      961 gcgagggacg cctgcgctcg atgcgcctca tctgtcctct ccatggcgcc tcgttcgata
     1021 tacgggatgg ccgtgtcctg gggtcgcccg ccataatccc acttcgcacc tacccggtac
     1081 gggtcgtgaa tggcacgatc gaaatttcgc tcactgtctg acaggctctt gcgctcgcaa
     1141 gtgggcaagc cagacgaacc agatcgcggg cagcgtgacg caagcgccca cgagaaaggg
     1201 agcgctcggg ccgagagcgg cgtagacagg cccggagatg aatggaccga tgacccgcgc
     1261 gagactgctg ctcgactggt aggtgcccat gacgctgcct cgatcgtgtg cctgcgcttg
     1321 cttggaagcg agtgcggagg cactcggatt gaacgagccc acccccacgc cgcagagcgc
     1381 gagtccgaaa ccggtcagcg ctagattggg cgccgcggcg actgtcacca ggccggcgac
     1441 ataggccagc acacccacgc tggcgagctt gacttcaccc agccgcggga ccagcaatct
     1501 gactccgcct ccttgcatca gcaccgcgag gcaggcgagg caaaacatga gcagtcccac
     1561 agtacgcggt ccaaagccga acttgttcaa cgcccagatc gcgaagatcg actcgagcac
     1621 ggcctgggag aaggtcacga gcaacgccgc ggtcgccagc cagcgcagcg cgggccgctc
     1681 gatcaacagg cgcagcggtc cagcgcgctc gcgtgcgatg tgctccccgc ggcgctcggc
     1741 ggtgttgctc tcgggaagga ggaaggccac caacgcgatc gcgaatagac tcaacccggc
     1801 cgagacggcg gcgggccgca cgaagctcgc ggcccgcaga tcattaccgg cgagcagccc
     1861 gccgatggcc ggccccagtg tgaagccgat gccgatcgcc gcgccgacca ttccaagcga
     1921 tgccgcgcgc tgctcgggct tgctcacatc ggaggcatac gcgaacgcgg ccgcgatgtt
     1981 gccagccatg aaaccaccca gaatgcgcga ggcaaacagc cagccaagtc catcagccag
     2041 gccgaggatg acataggagg cgcacgcccc ggcgaggctc gacatgagga tgggccggcg
     2101 cccaaaacga tcgctcaacc ggccccacag gggcgccgcg atgagttggc aaagagagta
     2161 gacgccgaac agagccgtga tcaccgccgg ggcggcgcca aaccgctcgg ccatgtaagg
     2221 cacgaggggg atcagga
//
LOCUS       DASRHN010000184         9248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_393439, whole genome shotgun sequence.
ACCESSION   DASRHN010000184 DASRHN010000000
VERSION     DASRHN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393439"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /gene="thrA"
                     /locus_tag="VFB37_07095"
     CDS             <1..1645
                     /gene="thrA"
                     /locus_tag="VFB37_07095"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HZO22257.1"
                     /translation="VGSGIPIWIRNTFAPEKPGTLICAQPQSALPVKGITSIERVALI
                     NLEGAGMIGVPGTAHRLFGALRDERISVILISQGSSEHSICCAIPQEQAERAAGVIRQ
                     AFERELKEGQIQNVDIDPELAILAVVGDGMAGTPGVAAKVFNALGTSTVNVRAIAQGA
                     SERNISVVVDGRHATRALRAVHAGLYLSPHTISIGLIGPGTVGRVLLDQLASQSGRLR
                     QEFKLDLRVRGLLASRRMLLSESGVPLAHWKQQFSEQAIEANFGRFLEHVRVDYLAHT
                     VIIDCTASAEVAKHNPQWLAAGIHIVTPNKKANSADLAFYRSLRDARRAGSAHYLYEA
                     TVGAGLPIVQTLRDLRETGDDIESVEGIFSGTLAYLFNLYDGRMPFSDIVREARQRGY
                     TEPDPRDDLSGMDVARKLIILGREMGLKLELSDVSVESLVPPGLDSGSIEEFMSRLPQ
                     HDAAMSARVAAAHGRGKVLRYVGRLTADGKATVGLMELDTKHPFANIALTDNVVRFAT
                     RRYCDNPLIVQGPGAGPEVTAGGVFADLLRLAAYLGARL"
     gene            1642..2598
                     /locus_tag="VFB37_07100"
     CDS             1642..2598
                     /locus_tag="VFB37_07100"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZO22258.1"
                     /translation="MSALQPESRHDRATAFAPASVGNVAIGFDILGFAVDALGDRVTV
                     ARREQPGVYISRVHGIAGELPRDPRRNTAGQALLAMRESLQLDFGFTLEIQKGIPLGS
                     GLGGSAASAVGAVVAANALLPAPCGKLELLKFAMQGEAVASGSLHVDNISPSLFGGLV
                     LTVGIDHPRVKQIPVPPEVRAVILHPHMFLSTKKARAILRRTVDLSAFVWQTANLAGF
                     ISGCYTNDLDMIRASLEDVVIEPQRQALIPGFQQVRRAALDAGALGCSISGAGPTIFA
                     WARLGDAPAVMQAMRHEFAQRAIELDEWIVEIDTAGARIVGT"
     gene            2595..3947
                     /gene="thrC"
                     /locus_tag="VFB37_07105"
     CDS             2595..3947
                     /gene="thrC"
                     /locus_tag="VFB37_07105"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZO22259.1"
                     /translation="MSTVSAQRPAAMRYASTRDPAGTAGFGEALQRGLAPDGGLYVPT
                     SWPHAQPEDLAQDRSLARLATRLLAPFVAGEPIAGELEAITDEAFSFPAPLVPLDSGP
                     KLSILELFHGPTAAFKDFGARFLAATFGRLRRSNPQPLTILVATSGDTGGAVAAAFHG
                     RAHFEVGVLFPKGLVSPTQERQLTCWGGNVRSIAVHGTFDDCQRLVKQAFTDSTLREQ
                     AELSSANSINLGRLLPQMVYYAAASLQIHRQHGERASFVIPSGNLGNALACIWARQVG
                     LPIERIVLAHNANRTVTDYLQGGEWRPRPSVATLASAMDVGNPSNMERLRAMFPDLDG
                     LRTAVTADSVEDVDIRRRIRLGFEQYGQIWCPHTATAAEVYHRLAARAGGADRLPGRW
                     VVVATAHPAKFPEIVEPLIGRVIPVPDALARLLARPTACTEIAADLAELRAALSRRRV
                     "
     gene            3947..4543
                     /locus_tag="VFB37_07110"
     CDS             3947..4543
                     /locus_tag="VFB37_07110"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HZO22260.1"
                     /translation="MEELQHLPPTAWALIAAAAVLGFAGSWIWRWYRQYRMRSALRAA
                     VTAAGTDHLVDMLVPDGMGGGFHVDFLLLTTRGILVIDLRDVQGNIFGGDQMAEWTVM
                     DGPRRFTFTNPQSALYDRIAAVRQVAGDVPVEGRIVFTRRGKFPKGLPKFTLMVDSLN
                     AEFPGGDHGPLSETLAHFNEGWDKVKKAVSPSYMTHMR"
     gene            complement(4598..6010)
                     /locus_tag="VFB37_07115"
     CDS             complement(4598..6010)
                     /locus_tag="VFB37_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO22261.1"
                     /translation="MTQEALQTFIGRIWDASILDRLIEYVRIPNKSPMFDPQWEKNGH
                     MEAAVGLMAEWCRRQPLPGLRVEVRRLPGRTPLLFVDVPGELPGCVLLYGHLDKQPEF
                     TGWLPGLGPWEPVVRDGKLYGRGAADDGYAVFSSLAAIAALKAQKVPLARCVLLIEAC
                     EESGSFDLPAHLEALGKEIEEPSLVVCLDAECGNYDQLWCTTSLRGNLTGTLHVRVLS
                     EGVHSGMATGIAPTPFRIVEQLIARLENAVTGDILLDELQVNLPKDRRAQVEAAARVL
                     GNTVAGKLPFAPGARAVSNDPVELLINSTWRATLAVTGADGLPEVGSAGNVLLPYLAF
                     KLSMRLPPTTDATRAAAAVKEALEADPPYGAQVRFEDASGVGGWNAPALAPWLEESIM
                     RASREIYGREAVHVGCGGSIPFMGMLGERFPKTQFFITGVLGPQANAHGPNEFLHIEY
                     AKKLTACVSLVLADHGRARP"
     gene            complement(6033..6896)
                     /locus_tag="VFB37_07120"
     CDS             complement(6033..6896)
                     /locus_tag="VFB37_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZO22262.1"
                     /translation="MNDSTRPPPAAATAQPAQLIDIGINLAHDSYDADREEVLARAAY
                     AGVVQMIVTGSNLHSTRAALELASTHTGRLFATAGVHPHHARELTGERLEELQQLAID
                     PGVVAVGECGLDYFRDFSPRPAQQAAFHRQLELAGRAGKPVFLHQRDAHEDFLAILRE
                     HRASFTGGVAHCFTGTSEQLASYLEHGLAIGITGWICDERRGTHLLALLPQIPADRLL
                     LETDGPYLLPRNLESKPASRRNEPAYLAHIAAVCARARGESAHTLAASSTAAARRVFG
                     LPETTSSHSNK"
     gene            6969..7745
                     /locus_tag="VFB37_07125"
     CDS             6969..7745
                     /locus_tag="VFB37_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22263.1"
                     /translation="MLCARAGRLAESVRILSCLSVLVIIRPASALCADASWLDVESRI
                     QYGYFTEDPRSIRGVMEKLTPQDPPDASRSYYAGLANYRLMLLLADRDRSHAKEAAER
                     CVASLDLALQSRSDFADALALQSACLDELASLEPWRSPFAASRSGAQLEKARHAEPRN
                     PRVLFLDAVESYERAKSTPQEREHALGEFRQAADAFEAERRQEDHIPGWGAAEAYLYL
                     ARCYLDRRSTLEARDALERALLIAPEFAQARRLMAKITAG"
     gene            7765..8046
                     /locus_tag="VFB37_07130"
     CDS             7765..8046
                     /locus_tag="VFB37_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HZO22264.1"
                     /translation="MNVPQTVTPPGSEGLGIRVVYMLLFAVAFWIVCWALALTALMQL
                     CVRLLNGRPSTEVKRFGEALGRYAKQVIEFLTFASEILPYPFTAWPAEA"
     gene            8043..>9248
                     /locus_tag="VFB37_07135"
     CDS             8043..>9248
                     /locus_tag="VFB37_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO22265.1"
                     /translation="MSLDRGELLQQLRIDREQRVDHIAGRRRGWLIAVAAAILILAAG
                     AAYVLWHGKRFAVEAATAVTPSTTAGPTAILQATGYVTARRQATVSAQITGTLTQVLI
                     EEGEHVKAGQVLARVEDTAQRAALGQANAQLEAAKALAVQLEAQLGQARRDLVRNQDL
                     VVRKLVSQQALETAQTQVDSLAAQLESQQKDVDLARAGVRAAQVQLDYTVVRAPFAGV
                     VIAKAAQAGEIISPISAGGGFTRTGVGTIVDMDSLEIEVDVNEAYINRVQPGQAAQAV
                     LDAYPNWTIPAHVIAIIPTADRSKATVKVRIGIDQKDSRILPDMGVRVSFLEAASKQA
                     EPPPGEAAAGSSVLVPSSSIVTRNGHSVVFRIEDDHVRASPVTPGQTFGDLRGVEGIA
                     NGTRVVREPP"
BASE COUNT         1537 a         3012 c         3105 g         1594 t
ORIGIN      
        1 cgtgggcagc ggcatcccga tctggattcg caacacattc gccccggaaa agcccggcac
       61 gctgatctgt gcgcaaccgc aatcggcact gccggtcaag ggcatcacca gcatcgagcg
      121 ggttgcgctg atcaacctcg agggggccgg catgatcggg gtgcccggta cggctcaccg
      181 gctgttcggc gcgctacgcg atgagcgcat ctcggtgatt ctcatctctc agggcagctc
      241 ggagcactcg atctgctgtg ccatcccgca ggagcaggcc gaacgcgccg cgggggtgat
      301 ccgtcaggcg ttcgaacgcg agctgaagga aggccagatc cagaacgtgg atatcgatcc
      361 cgaactggcc attctggcgg tcgtcggcga tggcatggcg ggcacacccg gcgtggccgc
      421 gaaagtcttc aatgctctcg gaaccagcac ggtgaatgtg cgcgccattg cccaaggcgc
      481 ctccgagcgc aacatttcgg tggtcgtcga cgggcgccat gccacgcgcg cgcttcgtgc
      541 cgtgcatgcc gggctgtacc tctcaccgca caccatctcg atcggcctca tcgggccggg
      601 caccgtcggc cgcgtgctgc tcgatcagct cgcctcccag agcgggcggc tgcgccagga
      661 gttcaagctc gacctgcgag tgcgaggtct gctcgcctcc aggcggatgc tgctttcgga
      721 gagcggggtg ccgctcgccc actggaagca gcagttcagc gaacaagcca tcgaggccaa
      781 cttcgggcgc ttcctcgagc acgtgcgggt cgactatctg gcccacacgg tcatcatcga
      841 ctgcacggcc agcgccgaag tggccaagca caatccacag tggctggccg ccggcattca
      901 catcgtcacc ccaaacaaga aagccaacag cgccgatctg gctttctatc gcagtctgcg
      961 cgatgcgcgg cgggcgggca gtgcgcatta tctgtacgag gcgaccgtcg gtgcagggct
     1021 gccgatcgtg cagaccctgc gtgatctgcg cgagacgggc gatgacatcg agagtgtgga
     1081 aggcatcttc tcgggcacgc tcgcgtatct tttcaacctc tacgatggac gcatgccctt
     1141 ttcggacatc gtgcgtgagg cccgccagcg aggctataca gagccggatc cgcgcgatga
     1201 cctctcggga atggatgtcg ctcgcaagct catcattctc ggtcgcgaga tgggcctgaa
     1261 actggagctc tccgatgtga gcgtcgagag tttggtgcca cccggactgg atagcggctc
     1321 gatcgaggag ttcatgagcc gcctcccgca acacgatgca gcgatgagcg cccgagtggc
     1381 cgcggcgcat ggccgcggca aggtgctccg ttatgttggt cgtctgaccg cggacggcaa
     1441 ggcaaccgtt ggactcatgg agctcgacac gaagcacccg ttcgcgaaca tcgccttaac
     1501 ggacaacgtg gtgcgctttg cgacgcgccg ctactgtgac aaccccctca tcgtgcaggg
     1561 gcccggtgcc ggaccggagg tcaccgctgg cggagtattt gcagatctgc tgcgacttgc
     1621 cgcgtatctg ggtgcgcggc tttgagcgcg ctgcagccgg agtcccggca tgatcgggcc
     1681 acggccttcg cgccggcctc cgtcggcaac gtcgccatcg gattcgacat cctgggcttt
     1741 gccgtggatg ctctcgggga tcgcgtgacg gttgcgcggc gcgagcagcc cggcgtgtac
     1801 atcagccggg ttcacggcat cgccggcgag ctgccccggg atccgaggcg caatacggcc
     1861 gggcaggctc tgctcgccat gcgcgagtcg ctgcagctgg acttcggctt cacgctcgag
     1921 atccagaagg gcattcccct gggctcgggc ctcggcggct ccgcggcctc cgctgtcggg
     1981 gcggtcgtgg ccgctaacgc actcctgcct gccccgtgcg gcaagctgga gctgctgaag
     2041 ttcgccatgc agggcgaggc ggttgccagc ggctcgctgc acgtggacaa catctcgccc
     2101 tcgctcttcg ggggcctggt actcactgtc ggaatcgacc atccccgggt caagcagatc
     2161 cccgtgccgc ccgaggtgcg cgcggtgatc ctgcatcctc acatgtttct ttcaaccaag
     2221 aaggcgcggg cgatcctgcg ccgcacggtg gacctttccg ccttcgtctg gcagaccgcg
     2281 aatctcgcag gcttcatctc cggctgttac accaacgatc tcgacatgat ccgggcatcg
     2341 ctcgaggatg tcgtgatcga gccgcagcgc caggcgctca ttcccggttt ccagcaagtg
     2401 cggcgtgcgg cgctcgatgc cggcgcactc ggctgctcca tctccggggc cggcccgacg
     2461 attttcgcct gggcgcgcct gggggatgca ccggcggtga tgcaggccat gcggcacgag
     2521 tttgcgcagc gcgctatcga gcttgacgaa tggatcgtcg agattgatac ggctggcgca
     2581 cggattgtcg gaacgtgagc accgtgagcg cacagcggcc tgcagccatg cgatatgcca
     2641 gcacccggga ccctgcggga acggcaggct tcggagaagc cctgcaacgc ggcctcgcac
     2701 ccgatggcgg cctatacgtt ccgacatcct ggccccatgc gcagccagag gatcttgcgc
     2761 aggaccgcag tcttgcgcgc ctggctacgc ggctgctcgc gccgttcgtt gcgggcgagc
     2821 cgatagccgg tgagctcgag gccatcaccg acgaggcctt cagcttcccg gccccgctcg
     2881 tgccgctcga ctcggggccg aagctctcga tactggagct cttccatggc ccgaccgcgg
     2941 cgttcaagga tttcggggcg cgctttctcg cggccacatt cgggcggctg cgtcgctcga
     3001 acccgcagcc gctgaccatc ctcgtcgcga cctcagggga cacagggggc gccgtagcag
     3061 ccgccttcca cggacgggcc catttcgaag tcggagtgct gttccccaag ggactggtct
     3121 cacccactca ggagcgccag ctcacctgtt ggggcggtaa tgtccgctcc atcgccgtgc
     3181 atgggacgtt cgatgactgt cagcgtctgg tgaagcaggc gttcaccgat tccaccttgc
     3241 gcgagcaggc cgagctctca tccgccaaca gcatcaatct cggtcgtctg ctgccgcaaa
     3301 tggtgtacta cgccgcggcg agcctgcaga tccaccgcca gcatggcgag cgggcctcgt
     3361 tcgtcatccc gagcggcaat ctcggcaacg cgctggcctg tatctgggct cggcaagtgg
     3421 ggctgccgat cgagcgcatc gtgctcgctc acaacgcgaa ccgtacggtc accgactatc
     3481 ttcagggcgg tgaatggcgg ccgcgtccga gcgttgcgac gctggcctcc gcgatggatg
     3541 tcggtaatcc gagcaacatg gagcgcctgc gggcgatgtt tccggacctg gatggcctgc
     3601 gtactgccgt gacggcagac agcgtggagg atgtcgacat tcgacggcgc attcgactcg
     3661 gcttcgagca atatggccag atctggtgcc cgcacacggc gacggcggcc gaggtctatc
     3721 atcggctggc ggcccgcgcg ggcggggcgg accggctccc cgggcggtgg gtagtcgtgg
     3781 cgacggctca tccggcaaaa ttcccggaga tcgtcgagcc gctgatcggc cgcgtcattc
     3841 ccgtgccgga tgcgctcgcg cggctgctgg cgcggccgac ggcatgcacc gagatcgcgg
     3901 ctgacctcgc ggagttgcgg gcggccctga gccggaggag ggtctagtgg aggagttgca
     3961 gcatcttccg cccaccgcct gggcattgat tgccgcggcg gccgtgctgg gcttcgcagg
     4021 ttcctggatc tggcgctggt atcggcagta ccggatgcgc tcggccctgc gtgccgcggt
     4081 gactgcggcc ggcaccgacc acctggtgga catgctcgtg cccgatggga tggggggcgg
     4141 tttccacgtg gatttcctgc tgctgacgac ccgcggcatc ctggtgatcg atctgcgcga
     4201 tgtccagggc aacatcttcg gcggcgacca gatggccgaa tggaccgtga tggatggtcc
     4261 gcggcgtttc accttcacca acccccagag tgcgctctac gaccgcatcg ccgcggtccg
     4321 gcaggtggca ggggatgtcc cggtggaagg ccggatcgtc ttcacccggc gcggcaaatt
     4381 tcccaaggga ctgccgaaat tcactctgat ggtcgactcg ttgaatgcgg agtttccggg
     4441 gggtgaccat ggccccctct cggaaactct ggcgcacttc aacgagggct gggacaaggt
     4501 caagaaagcc gtcagtccga gctacatgac gcatatgcgc taggcctctc tttgcaggtg
     4561 ctcgggcaca tccatgtgcc ttcagccctg cgatgcctca cggcctcgcg cggccatgat
     4621 cagccagcac cagcgagacg caggcagtga gcttcttggc gtattcgatg tgcagaaact
     4681 cgttgggccc atgagcattg gcttgcggcc cgagcacccc ggtgatgaaa aactgcgttt
     4741 tcggaaagcg ttcaccgagc attcccatga aggggatgga gccgccgcaa ccgacatgca
     4801 cggcctcgcg accgtagatc tcgcgcgagg cgcgcatgat ggattcctcg agccacggcg
     4861 ctaaggccgg cgcattccaa ccgccgacac ccgaggcgtc ctcgaagcgc acctgtgcgc
     4921 catatggggg gtcggcttcc agcgcctcct tgacggcggc cgcggcgcgc gtggcatcgg
     4981 tggtaggcgg caggcgcatt gacagcttga acgcgaggta cggcagcagg acgttgccgg
     5041 ccgagcccac ctcgggcagg ccgtcggctc cggtgaccgc cagtgtggcg cgccaggtgc
     5101 tgttgatcag caattcaacc ggatcattcg atacggcccg ggctccaggg gcgaagggca
     5161 gcttgccggc gacagtgttg cccagcacgc gtgccgccgc ttcgacctgc gcgcggcgat
     5221 ccttggggag gttgacctgc aactcatcga gcaggatgtc gccggtgacc gcgttctcca
     5281 gccgtgcgat gagctgctcc acgattcgaa acggcgtcgg cgcgatgccc gtggccatgc
     5341 ccgagtgcac gccctcgctc agtacgcgca cgtgcagcgt gccggtgaga ttgccgcgca
     5401 gcgatgtcgt gcaccagagc tgatcgtagt tgccgcactc tgcatccaga cacaccacga
     5461 gcgagggctc ctcgatttcc ttgccgaggg cttccaggtg ggccggcaga tcgaagctgc
     5521 cgctttcctc gcacgcctcg atcaacaaga cacaccgtgc aagcgggacc ttctgtgcct
     5581 tgagtgcagc gatagccgcg agtgaggaga acaccgcata tccgtcgtcc gcagcccctc
     5641 gaccgtagag cttgccgtcc cgtaccaccg gctcccacgg cccaagcccc ggcaaccagc
     5701 cggtgaactc cggctgcttg tcgagatgac cgtacagcaa cacgcacccg ggcagctcgc
     5761 ccggcacatc gacgaagagc aggggggtgc gcccggggag ccggcgcacc tcgacgcgca
     5821 gtccggggag gggctggcgt cggcaccatt ccgccatgag cccgaccgcc gcttccatgt
     5881 ggccgttctt ctcccactgg ggatcgaaca tcggggactt gttgggaatg cgcacgtact
     5941 cgatgaggcg gtcgagaatc gacgcgtccc agatgcggcc gatgaaggtc tggagtgcct
     6001 cttgtgtcac gactatctcc gttattaatc agctatttat ttgaatgaga tgaagttgtt
     6061 tctggtaagc cgaagacccg gcgcgccgcc gccgtgctgc tcgccgccag cgtgtgcgcg
     6121 gattctccgc gggcgcgggc gcacaccgcg gcaatgtgcg cgagataggc aggctcattc
     6181 cggcgcgaag cgggcttgga ctcgaggtta cgcggcagca ggtagggacc gtcggtctcg
     6241 agcagcagac gatcggcagg gatttgcggc agcagggcca gcagatgggt cccgcgccgc
     6301 tcatcgcaga tccagcccgt gatgccgatg gccagtccgt gctcgaggta gctggcgagc
     6361 tgctcgctcg ttccggtgaa gcagtgcgcc actccgccgg tgaagctcgc gcggtgctcg
     6421 cgcaggatgg cgaggaaatc ctcatgcgcg tcgcgctgat ggaggaacac cggcttgccc
     6481 gcccggcccg cgagctcgag ctgccgatga aaggcggcct gctgcgcggg ccgcggggag
     6541 aaatcccgaa agtaatcgag cccacactca cccaccgcca cgaccccggg gtcgatggcg
     6601 agctgctgca gctcctccag gcgctcgccg gtgagctcgc gcgcgtggtg aggatgcacg
     6661 cccgcggtgg caaacagccg ccccgtgtgg gtactcgcca gctcgagagc cgctcgcgtg
     6721 ctgtggaggt tcgagccagt cacgatcatc tggacgacgc cggcgtacgc cgctcgggca
     6781 agcacttcct cacgatcggc gtcgtagctg tcatgggcca gattgatgcc gatatcgatc
     6841 agctgggcag gctgagccgt ggcggcagcc ggcgggggcc ttgtgctatc gttcatcatg
     6901 cgcgagacac attttacggc acgccccggt acgcgatgtc cttgagggaa cagtcttcgg
     6961 attcacgagt gctgtgtgct cgcgccggac ggcttgccga gagtgtccga atcctgtcct
     7021 gtctcagtgt gctggtcatc atcaggccag cgagcgccct gtgcgcggat gcgagctggc
     7081 tcgacgtgga gagccgcatc cagtatggat acttcaccga ggatccgcgc tcgatccgcg
     7141 gcgtcatgga aaaattgaca ccgcaggatc cgcccgatgc ctcgagaagc tactacgcag
     7201 gtctggccaa ttatcgcctc atgctgctgc tggcggaccg cgatcgcagc cacgccaagg
     7261 aggccgcgga gcgctgcgta gcgagcctcg atcttgcgtt gcagagccgc agcgactttg
     7321 ccgacgcgct ggcgctgcag tccgcctgcc tcgatgagct ggccagcctc gagccttggc
     7381 gcagcccgtt cgccgcctcg cgcagtggag cgcagcttga gaaggcgcgc catgccgagc
     7441 cgcgcaaccc tcgggtgttg tttctcgatg cagtggaaag ctacgagcgg gcgaaatcga
     7501 cgccgcagga gcgcgagcac gcgctggggg aattcagaca ggcggccgat gcattcgagg
     7561 ccgagcggcg gcaggaagat cacatcccgg gctggggagc ggcggaagcc tacctgtacc
     7621 tcgcgcgatg ttatctcgac cggcgaagca ccctggaggc gcgcgatgcg ctcgagcgcg
     7681 cgctgctcat tgcgccggag tttgcccagg cccggcggct catggcgaaa attacagcgg
     7741 gatgaccgca agcgggactc catcatgaat gttccacaga ccgtcacgcc cccgggctcc
     7801 gaggggcttg ggatccgtgt cgtatacatg ctgctctttg ccgtggcttt ctggatcgtg
     7861 tgctgggcgc tcgcgctcac ggcgctcatg cagttgtgcg tgcggctgct gaacggccgg
     7921 cccagcacgg aagtgaagcg attcggtgag gcgttggggc gctacgcgaa acaggtcatc
     7981 gaattcctga ctttcgcctc cgagatcctg ccctatccgt tcacggcctg gccggccgag
     8041 gcgtgagttt ggaccgcggc gagctgctgc aacaactgcg gatcgatcgc gagcagcgcg
     8101 tcgatcacat cgcagggcgc cggcgcggct ggctgattgc ggtagctgcg gcgatcctga
     8161 tcctggcggc aggcgctgct tacgtactct ggcatggcaa gcgtttcgcc gtcgaagcgg
     8221 ctaccgccgt gacgccttcg accactgccg gacccacggc catcctgcag gcgacgggct
     8281 acgtgaccgc acggcgtcag gcgaccgtct cggcacagat caccgggacg ttgacccagg
     8341 tcctgatcga agagggcgag cacgtgaagg ccggccaggt gctggcccgc gtggaggaca
     8401 cggcgcagcg agccgctctc ggtcaggcga acgcgcagct cgaggccgcc aaggcgctcg
     8461 cggtacaact cgaggcgcag ctcgggcagg cgcgtcgcga cctggtccgc aatcaggacc
     8521 tggtggtgcg caagctcgtg tcgcagcagg cgctcgagac ggcccagacg caggtggaca
     8581 gcctcgccgc ccagctcgaa tcgcagcaga aggatgttga tctggctcga gcaggggtgc
     8641 gggccgccca ggtccagctc gattacaccg tcgtccgagc acccttcgca ggcgtggtga
     8701 tcgccaaggc ggcgcaggcc ggcgagatca tctcgcccat ctctgccggc ggggggttca
     8761 cccgcaccgg tgtcggtacg atcgtggata tggactcgct cgaaatcgag gtggacgtca
     8821 acgaggccta catcaaccgc gtgcaaccgg ggcaagccgc acaggccgtg ctcgatgcgt
     8881 atccgaactg gacgatcccg gcgcacgtga tcgcgatcat cccgacggcc gaccgcagca
     8941 aagcgacggt caaagtgcgc atcggcatcg atcagaaaga ctcgcgaatc ctgcccgaca
     9001 tgggcgtccg cgtctcattc ctcgaggcgg cctcgaaaca ggccgagccg ccaccagggg
     9061 aggctgcagc gggctcctcg gtgctggtac cgtcctcgtc gatcgtcacg cgtaatggtc
     9121 acagcgtggt cttcagaatc gaggatgatc atgtccgtgc cagccctgta accccggggc
     9181 agaccttcgg agacctccga ggcgtggaag gcatagcgaa cggcacgcgt gtggtgcgcg
     9241 aaccgccg
//
LOCUS       DASRHN010000185         8214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1049868, whole genome shotgun sequence.
ACCESSION   DASRHN010000185 DASRHN010000000
VERSION     DASRHN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049868"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..2960
                     /locus_tag="VFB37_07140"
     CDS             330..2960
                     /locus_tag="VFB37_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22266.1"
                     /translation="MKANQKVYCAVAAILSAHALAATAATPADATPADEESSTGITEV
                     VVTAQRRSENIQDVPIAIQALTSDTLKQLNVQTFDDYVKYIPSVTFKSNGPGQSLIYM
                     RGLSSGASAGSQSTGTIGGFPNVAIYLDDQSGQLPGRNLDIYAADLERIEVLEGPQGT
                     LFGAGAEAGVIRYITNKPKLNTTEASTTASYGTTAHGDNNSSIQAVLNLPVIPDTLAL
                     RAVVYDDQRGGYIDNVPGTFTRRNTDLGIYYANYPTGCSATHNCQVPPGSPVANNSSL
                     VQNNFNPVTYQGIRASGLWQINDNWSALVTQIYQDMNSQGVFYQMPFASDGAKLQPLQ
                     VTLFSPSYDKDKFENTSWTLNGTVGPLKAIYTGGYLTRSLDARNDYTNYARGVYADYY
                     QCYGASAGSSGQSRCFSPVAAWHETLRNTHQSHEIRLSTPDDWRTRAIVGAFWEQVKI
                     FDNTDWLYKTLPPCTTVGQLGCMTDVAGAPGATANIPGTRNDNDAFFEDVQRGYKQYA
                     AFASVDFDIIPKVLTVTAGTRWYDFQNVQTGSVVSSFGCENAGPAPCTAGADSINGEN
                     EHSSYKGFKSRANLSYHITPDTLLYYTWSQGFRPGYFNRKGGCHVKDAQGIPEFCIPL
                     PIAPDSLTNNELGWKTEFLDHRLQVNGAVYQEQWNNVQVGFFDPGQLGNLTFGTNGQN
                     FRVRGAELQVEARVTQGFTVTGAITYNKSEQTNSPALIANNPALLANPLSAPEFGKPI
                     TQLQNPYGAVGSPTANSPLFQGNLRARYEFAWNQYNLFAQIGATYSTSSFTQTSTNPS
                     LSAGSSINTTLLRFENPPYGLIDGAVGIAKDQWTVQLFGENLADRNVSLFTSTGQFVV
                     TQTPTRPRVLGLQVGYKFSP"
     gene            3155..5155
                     /locus_tag="VFB37_07145"
     CDS             3155..5155
                     /locus_tag="VFB37_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO22267.1"
                     /translation="MQSATANTPEPPVEGEVARIRGLMEAGRFEPALAAAASLARRVP
                     ENRDVLYMLAVCQRYLHRIPEALATLVQLQEVHPRFSRLYQERGHCYVALRDAPHAIE
                     AFLQAVNMNPALPASWRTLQALYRLTGNMKDAETAGAHMDTLARLPAEVVTANGMFAD
                     GELALAEQLIRSFLLKHGDNIEAMRLLARIGMELDVLDDAEVLLEAVLRLAPDYDAAR
                     HDYASTLLRRYKPVQAIEELDKLIRRQPDNRQFRTTYANAVVGLGDHERALALYRELL
                     PSSGQPAEVHLSIAHSLKTLGRQAEAVDAYRASAAARLNYGDAYWSLANLKTYRFTDA
                     EIEQMRAEESSKRTQQTDRYHLCFALGKALEDRGDFAASFAYYARGNALKKSEIRYRP
                     EPIERNTRLQKQICTQDFFAQRQGWGSPRPDPIFIIGLPRSGSTLIEQILASHSQVEG
                     TMELAEIPRLVHQLSGRENYADEPRYPQALGKLSADEVLKYGEAYLANTTIYRTGKPF
                     FIDKMPNNFRHLGLIHLILPRAKIIDARREPMACCFSNFKQLFANGQEFTYSLEDIGR
                     YYRTYLELMAHWDAVLPGRVLRVQHEDLVEDLEGHVRRLLEFCELPFEPACLEFYRTE
                     RSVRTASSEQVRQPIFKEGLDQWRNFEPWLGPLREVLAAPVS"
     gene            complement(5148..6299)
                     /locus_tag="VFB37_07150"
     CDS             complement(5148..6299)
                     /locus_tag="VFB37_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZO22268.1"
                     /translation="MNAAPAVVVSSALTHRIATIEISHHRLQFQPAFHPSWDSRPRTH
                     FDATIVRVTTDTGFAGHGSGDLMVGFQGHESLFIGQDALALERHYRTLANIDFHYGRC
                     WPLDLALWDLAGKITGQPCWKLLGGLSHRVQAYASSGTLRDPGALAEAAERFLAQGFR
                     ALKLRFHRADWRDDIRALEAVRARIGSRLELLVDCNQGWRQPWDTQAAWTLKDALAVA
                     RELEKLKVYWMEEPLHRGDKAGMRDLRSSTDVRIAGGEMTRQAHELRDLIVGGCIDVL
                     QPDAALVGGITGLRRAALMAQEHDLIFTPHTWTNGMGVMANTHLVAGLCQAPFIEYPY
                     DPPEWNLERRDYMMQQPLEVDREGWLSLSDAPGMGYALDEARLARTRIS"
     gene            6558..7541
                     /locus_tag="VFB37_07155"
     CDS             6558..7541
                     /locus_tag="VFB37_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZO22269.1"
                     /translation="MSKKSIDRRWLPLNALRAFEGVAKHGSFTAAAHALLISQSALSR
                     HVIALERLIGVQLFERRPHSLTLTKAGQHLLPAVVKSFDRLEYALDDIRNEGAPAQRT
                     LRVQMPPSFAVQLAVPILRDFRQASSEVDIDLISPYRVGPALGDVDVAVLYSKPMVTD
                     LVTDLLWLVRPSVLCHPDIAARHASKDLASFIEANELVHVRINDLPRHHTWTQFVRQS
                     GLRPINVERGLVVDTAVLAVQYALSGQGIAICDTNLFAEEIRQGHLVKPFDVTLDDGY
                     GYYLVTHPEGLGDTAIGMFRSWLIERFGSGAAAAESDVTSESPVHLIVSNE"
     gene            7574..>8214
                     /locus_tag="VFB37_07160"
     CDS             7574..>8214
                     /locus_tag="VFB37_07160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO22270.1"
                     /translation="MKNAAKVVVVGGGVVGASVLYHLTKAGWHDVLLIEREELTSGST
                     WHAAGGMHTINGDPNVAKLQQYTINLYKELERISGQSCGMHITGGVMLAGTPERMDWL
                     RMTKARGRYLGMELELVSSEEAAKLFPIMDPKHFVGGLYDPIEGHIDPYGVTHAYAKS
                     AQLAGAEVVRKTRVVDLKPRPDGSWDVITDHGNVHAEHVVNAGGLWAREVGRMV"
BASE COUNT         1592 a         2644 c         2566 g         1412 t
ORIGIN      
        1 gcgatctcat gttgcgatgg cgcatcgcgc ggaggggccc agaagttgtg gacaagcggg
       61 ccgggtgccc gcaggattgg gcacctgcga cgcgttggtc gcatgcgggg cgttggttgg
      121 attcccagtg caggaatcga agaaggccat gatttttttg gatggcccga tccaatttct
      181 ccgcttgcct cggttgacgg gactatgaaa cgtttgggcg ggggggcctt cggccgcaag
      241 ctcgtgttgc ccgtgcgcta cagccgggga ccgagccgga accacggcgg ctctcccggg
      301 acgacagcca atattcgggg gagagcagct tgaaagcgaa ccagaaggtc tactgtgccg
      361 tcgccgccat cctgagcgcg cacgcgctgg ccgcgaccgc cgcaacaccc gccgacgcga
      421 ctccggcgga cgaggaatcc agcaccggca tcaccgaggt cgtggtgact gcgcagcgtc
      481 gctcggaaaa catccaggac gtgccgatcg cgatccaggc gctgacgtcc gacacgctga
      541 agcaactcaa cgtgcagacg ttcgatgact acgtgaaata catcccgagc gtgacattca
      601 agtccaacgg cccgggacaa agcttgatct acatgcgcgg tctgagctcg ggcgcatccg
      661 caggcagcca gtcgactggc acgatcggtg ggttcccgaa cgttgccatc tatctcgatg
      721 atcaatcggg ccagctgcct ggccgtaacc tggacatcta cgccgccgac ctggagcgca
      781 tcgaggtcct ggaaggtcca cagggcacgc tcttcggtgc gggcgcggaa gcgggcgtga
      841 tccggtacat caccaacaag cccaagctca acacgaccga ggcgagcacg acggccagct
      901 atgggaccac cgcgcacggt gataacaaca gttccatcca ggccgtactc aacctgccgg
      961 tgatccccga tacgctggct ctgcgcgcgg ttgtctatga cgatcagcgc ggaggataca
     1021 tcgacaacgt gccgggaacg ttcacgcgcc ggaacaccga cctgggcatc tactacgcaa
     1081 actacccgac gggttgctcg gcgacccata actgccaggt gcctcccggc agccccgtgg
     1141 cgaacaacag ctcgctcgta cagaacaact tcaatccagt cacctaccag ggcatccgcg
     1201 cctcgggact gtggcagatc aatgacaact ggagcgcgct ggtcacgcag atctaccagg
     1261 acatgaactc gcagggcgtg ttctatcaga tgccgtttgc ttccgacggg gcgaagctgc
     1321 agccgctgca agtcacgctg ttcagcccgt cgtacgacaa ggataagttc gaaaacacct
     1381 cctggacact gaatgggacg gtcgggccgc tcaaggccat ttataccggc gggtacctga
     1441 cccgcagcct cgatgcgcgc aacgactaca ccaactacgc gcgcggcgtg tatgccgact
     1501 actaccagtg ctacggcgcc tccgccggct cgagtggcca gtccaggtgc ttctctccgg
     1561 tcgctgcctg gcacgagaca ctgcgcaaca cccaccagag ccacgagatt cggctcagca
     1621 ctccggatga ttggcgcacc cgcgccatcg tcggtgcctt ctgggagcag gtgaagatct
     1681 tcgacaacac ggactggctg tacaagacct tgccgccgtg caccacggtg ggccagctgg
     1741 gttgcatgac cgacgtcgct ggggcgcccg gtgcaaccgc gaacatcccc ggcacgcgca
     1801 acgacaacga cgcattcttc gaggacgtgc aacgcggcta caagcagtat gctgcattcg
     1861 cctcagtgga cttcgacatc atcccgaagg tcctgacagt cacggccggc acgcgctggt
     1921 acgacttcca gaacgttcag accggcagtg tggtcagctc cttcggctgc gaaaatgccg
     1981 gccctgcgcc ctgcacggct ggggccgaca gcatcaacgg cgagaacgag cactcgagct
     2041 acaaggggtt caagagtcgc gccaacctca gctaccacat cacacccgat accctgctgt
     2101 actacacgtg gtcgcaggga ttccgtccgg gctacttcaa tcgcaaaggt ggatgtcacg
     2161 tcaaggatgc gcaagggata cccgagttct gcatcccgct gccgattgcc ccggactcgc
     2221 tcaccaacaa cgagctcggt tggaagaccg agttcctgga tcatcgcctg caggtgaacg
     2281 gggcggtcta tcaggagcag tggaacaacg tgcaggtcgg cttcttcgac ccggggcagc
     2341 tcggcaatct gaccttcggc acgaacggtc agaacttccg tgtccggggc gccgagctgc
     2401 aggttgaagc gcgggtgacg cagggcttca cggtcacggg cgccataacc tacaacaaga
     2461 gcgagcagac caactcgccg gcgttgatcg ccaacaatcc tgcgctgctg gccaacccgc
     2521 tctccgcccc cgagttcggc aagccgatca cgcagttgca gaatccgtac ggtgccgttg
     2581 gcagcccgac ggcgaactca ccgctcttcc agggcaatct gcgcgcgcgt tacgagtttg
     2641 cgtggaacca gtacaaccta ttcgcgcaga tcggggccac gtactccacc agctccttca
     2701 cgcagacgag cacgaatccc tcgctctccg ccggcagctc gatcaacacc acgctgctgc
     2761 gattcgagaa cccgccgtat gggctcatcg acggagccgt gggcattgcg aaggatcagt
     2821 ggacggtgca gctgttcggc gagaatctgg ccgatcgcaa tgtgagtctg ttcacctcga
     2881 cgggccagtt cgtcgtgacc cagactccca cccggccgcg ggttctgggt ctacaggtcg
     2941 ggtacaagtt ctcgccctag tccattggaa aaatcgcggc agcgattttt cggacatcgc
     3001 cttcggcgtc gggcgcgaaa ggcgcgactt tcgcgccctg cgctgctgcg aacaagggtg
     3061 aacgggccct gatcagggga ttgccgctcg agccgacaga ggcgcggatg gatgggtaga
     3121 atcgggggcg gagaagtccg cccccgatcc agccatgcag tcagcgaccg caaatactcc
     3181 ggagcccccc gtcgagggcg aagtcgcccg catccgcggc ctcatggagg caggccggtt
     3241 cgagccggcg ctcgccgcag ccgcgtcgct ggcgcgccgg gtgcccgaga accgcgacgt
     3301 gctctacatg ctcgcggtgt gccagcggta tctgcataga attcccgagg cgctcgccac
     3361 gctggtgcag ctccaggagg tgcatcccag gttcagccgc ctctaccagg agcgcggcca
     3421 ctgctacgtg gcgctgcggg atgcgccgca cgccattgaa gcgttcctgc aggcggtgaa
     3481 catgaatcct gccctgccgg cgagctggcg gacactgcag gcgctctatc ggctcacggg
     3541 gaacatgaag gacgccgaga ccgccggcgc gcacatggac accctggctc gcctgcccgc
     3601 cgaagtcgtg acggcgaatg gcatgtttgc cgatggcgag ctggcgcttg ccgagcagct
     3661 catccgcagc tttctcctca agcatggtga caatatcgag gccatgcgcc tgctcgcgcg
     3721 gatcggcatg gagctcgatg tgctcgatga tgcggaggtt ttgctggagg cggtgctgcg
     3781 gctcgcgccc gactacgatg ccgcccgtca cgactatgca agcactctgc tgcgaagata
     3841 caagcccgtc caggcgatcg aggagctgga caagctcatt cgccgccagc ccgacaaccg
     3901 gcaattccgc acgacttacg ccaatgcagt cgtggggctg ggcgatcacg agcgtgcgct
     3961 ggctctgtat cgcgagctgc tcccttcatc cggtcagccg gccgaagtgc atctctcgat
     4021 cgcgcattct ctcaagactc tgggaagaca ggcggaagcc gtggacgcct accgggcctc
     4081 cgccgccgcc cgtctcaact acggggatgc ctactggagt ctggccaatc tcaagacgta
     4141 ccgattcacg gacgccgaga tagagcagat gcgagccgag gagagcagca agcggacaca
     4201 gcaaaccgat cgctaccacc tgtgcttcgc actcggtaag gcgctcgagg accgcggcga
     4261 tttcgcagcg tcgttcgctt attacgcccg cggcaacgcg ctcaaaaaat cggagatccg
     4321 ctaccggccc gagccgatcg agcgcaacac ccgcctgcag aagcagatct gcacgcagga
     4381 cttctttgcc caacggcagg gctggggctc gccccgaccc gacccgatct tcatcatcgg
     4441 cctgccccgc tcgggctcga ccctgatcga gcagattctg gcttcccatt cccaggtcga
     4501 aggcacgatg gagctcgcgg agatcccgcg cctcgttcat cagctcagcg gccgcgagaa
     4561 ttacgcggac gagccgcgct atccgcaggc actcggcaaa ttatctgccg acgaggtcct
     4621 gaagtacggc gaggcatacc ttgccaacac cacgatctac cgcaccggca agccgttctt
     4681 catcgataag atgcccaata acttccgcca cctgggcctt attcatctga tcctgcccag
     4741 agcgaagatc atcgatgccc gacgcgagcc gatggcctgc tgcttcagca acttcaagca
     4801 gctctttgcc aatgggcagg agttcacgta cagcctcgag gacatcgggc gctattaccg
     4861 cacctacctc gagctgatgg cgcactggga tgcggttcta cccggcagag ttctgcgtgt
     4921 gcagcacgag gatctcgttg aggatctgga aggccatgtc agacggctgc tcgagttctg
     4981 tgagctgcca ttcgagccgg cgtgcctcga gttctaccgc accgagcgca gcgtgagaac
     5041 ggccagctcc gagcaggttc gtcagcccat tttcaaggag ggactcgacc agtggcgcaa
     5101 cttcgaaccc tggctcggtc cgctcagaga ggttctggcc gcgccggtca gctgatccgc
     5161 gtgcgcgcaa gccgcgcttc atcgagcgcg taacccattc cgggcgcatc gctcagactc
     5221 agccatccct cgcgatcaac ctcgagcggc tgctgcatca tgtaatcgcg gcgctcgaga
     5281 ttccattcag gtgggtcgta gggatactcg atgaagggag cctgacagag cccggcgacg
     5341 agatgcgtgt tggccatgac gcccatgccg ttcgtccagg tgtgcggcgt gaaaatgaga
     5401 tcatgctcct gggccatgag agccgcgcgt cgcaagcccg tgatgccgcc gaccagcgcc
     5461 gcatcgggct gcaggacatc gatacatccg ccgacgatca gatcgcgcag ctcatgtgcc
     5521 tggcgggtca tctcgccccc ggcgatgcgc acgtcagtcg atgaccgcaa gtctcgcatg
     5581 cccgccttgt cgccccgatg gagcggttcc tccatccagt acaccttgag cttctcgagc
     5641 tcccgtgcga ccgccaatgc atccttgaga gtccaggccg cttgcgtatc ccacggttgc
     5701 cgccagccct gattacagtc gaccagcagc tccaggcgcg atccgatgcg tgctcttacc
     5761 gcctcgagcg ccctgatgtc gtctcgccag tccgcacggt ggaatcgcaa tttcaaggca
     5821 cgaaaaccct gcgcgagaaa gcgctcggct gcctcggcga gggctcccgg atcgcgcagc
     5881 gtccccgagg aggcgtacgc ctgcacccga tgcgacaaac cgcccagcag cttccagcag
     5941 ggctgcccgg tgatcttgcc agccagatcc cacagcgcca gatccagcgg ccagcagcgg
     6001 ccataatgga agtcgatgtt cgcaagcgtg cggtagtggc gctcgagcgc cagggcatcc
     6061 tgtccgatga acaggctctc gtggccctgg aagccgacca tcagatcacc ggagccgtga
     6121 ccggcgaacc ccgtatcggt ggtgacccgc acgatcgtgg catcgaagtg cgtgcgcggc
     6181 cggctgtccc agctcggatg aaatgccggc tggaactgca ggcggtgatg cgagatctca
     6241 atcgtggcga tccggtgggt cagcgcagag gagaccacga cggccggggc agcattcatc
     6301 gccagcgttc ctccaggtgc gcgcggtcgc ggtgctgcct gcgccggagc ggcagccaac
     6361 cctcccgcgc gcagactttc acagatgcag gacacctcaa agccgagaaa ttggctaccc
     6421 gcatcgaaaa aaggcatcgg aacgccggtg ggccggccgg ctggccacgc tagaatcgga
     6481 gcaagccggg agagcacgcg cggaatcatg agcgttctgc tgcgcaacct gcggcgccgc
     6541 ttcgggagga acgttccgtg agcaaaaaat ccatcgaccg gcgctggctg ccgctgaacg
     6601 cgctgcgggc cttcgagggc gtcgccaagc acggcagctt cactgcggcc gcacatgcgc
     6661 tgctcatctc ccagagcgcg ctgtcccgac acgtgatcgc gctggagcgg ctaatagggg
     6721 tgcaactctt cgagcgccgt ccgcactccc tgaccctgac caaggccggg caacacctcc
     6781 tgccagcggt ggtgaaatcc ttcgatcgcc tggaatacgc gctcgatgac atccgcaacg
     6841 agggggctcc ggcgcagcga accttgcgcg tgcagatgcc gccgagcttc gcggtgcagc
     6901 ttgcagtgcc gatcctgcgc gatttccgtc aagcgagcag tgaggtggac atcgacctca
     6961 tcagcccata ccgcgtcggc cccgccctgg gcgatgtgga tgtggcggtg ctgtattcaa
     7021 agccgatggt cacggacctg gtgaccgatt tgctgtggct cgtacgtccg agtgtgctgt
     7081 gccatccgga catcgccgca aggcatgcga gcaaggatct agcgagcttc atcgaggcga
     7141 acgagctggt tcacgtgcgc atcaacgatc tgccccggca tcacacgtgg actcagttcg
     7201 tgcgccagtc cgggctgcgg cccatcaatg tggagcgagg tctcgtggtg gatactgcgg
     7261 tcctggcagt gcagtacgcc ctcagcgggc aggggatcgc gatctgcgat acgaacctgt
     7321 ttgcggagga gatccgccag gggcatctcg tgaagccttt cgatgtcact ctcgatgacg
     7381 ggtacggcta ttacctcgtc acgcatccgg aagggctggg ggacactgcg atcggcatgt
     7441 tccgctcctg gctcatcgaa agatttggca gcggcgcagc ggccgccgag tcggacgtca
     7501 cgtccgagtc accggtacat ctgatcgtat ccaacgagta gatccgttcc gcgtttcgaa
     7561 tgagcgagtt gcaatgaaga atgctgcgaa ggtggtggtg gttggcggtg gtgtggtcgg
     7621 ggcgagcgtt ctgtatcacc tgaccaaggc aggatggcac gatgttctgc tgatcgagcg
     7681 cgaagagctg acgtcgggat ccacttggca tgccgccggc ggcatgcaca ccatcaacgg
     7741 tgatccgaac gtcgccaagc tgcagcagta cacgatcaat ctctacaagg agctggagag
     7801 gatctcaggg caatcctgcg gcatgcatat caccggcgga gtgatgctgg cgggtacgcc
     7861 tgaacggatg gactggctgc ggatgaccaa ggcgcgcggc cgctacctcg gcatggagct
     7921 cgagctggtc tccagcgagg aagcggcgaa gctgttcccg atcatggatc ccaagcattt
     7981 cgtagggggc ctgtacgatc cgatcgaagg tcacatagac ccgtacggag tcacgcacgc
     8041 gtacgcgaaa tcggctcagc tcgccggggc agaggtggtc cgcaagaccc gagtggtcga
     8101 tctcaagccg cggcccgacg gctcgtggga tgtcataacc gaccacggca acgtgcatgc
     8161 ggagcacgtt gtcaatgccg gtgggctctg ggctcgggaa gtcgggcgca tggt
//
LOCUS       DASRHN010000186        32049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_887599, whole genome shotgun sequence.
ACCESSION   DASRHN010000186 DASRHN010000000
VERSION     DASRHN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 32049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887599"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFB37_07165"
     CDS             <1..220
                     /locus_tag="VFB37_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22271.1"
                     /translation="PAQRVRRPPRPKPGLVIAESAEHEGFRTRDVLAALRGVTLALDM
                     HFQSLDIEPHELATAARVLAAILYNRAF"
     gene            complement(310..852)
                     /locus_tag="VFB37_07170"
     CDS             complement(310..852)
                     /locus_tag="VFB37_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22272.1"
                     /translation="MTVKVFYASEGKLVKAPDGQPGTIFDKLFASAEAARAAPFPEGA
                     VSATFSDVGGSWYRKRDSAWEFHREDFDRLRAARDDAEQVARIRLHVRIPSELDEFLK
                     TATDSSLKHCLFYNENAQRATYVAANRSEVVCYFVEPLKRVLADLVEMKLRRTDERIW
                     QDTSAFRAIVDQVRRDHTGG"
     gene            complement(1407..1733)
                     /locus_tag="VFB37_07175"
     CDS             complement(1407..1733)
                     /locus_tag="VFB37_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22273.1"
                     /translation="MSATHQDLAAIRARHRAEFGGSAMLLLTGPKGEDAATWLADEVL
                     AGHDELRKQIGELQAVAARGGTARELGEQMQVMYEVLIYQLGHLALAGIHAAQRDVLA
                     RYQADQ"
     gene            complement(1730..3733)
                     /locus_tag="VFB37_07180"
     CDS             complement(1730..3733)
                     /locus_tag="VFB37_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22274.1"
                     /translation="MSQDISSNITADNSQFLASLEEAKGAAQSFFQSITDQTAGLGGA
                     LEDIQSKFSAAFAFTGLAAATAGLAKVSEIINSMGSEAIQISTLSNVLGVTVEQFQAL
                     RLAGEEAGAGPEVLARGSERLAQTLDEARAGSQQAIDKMLALGVSIEQIQSPTFQLND
                     LLQLLHDRLTNASTAQAEHDALLQIYGSRSALMIAALEHYVGSTQGVASANERVNALT
                     GEQNTALVDLKRRWDELSDSLSNTFKKAVLGAIDGFNSLQGAASTAQLSKATLGGAVG
                     TNPFAQMGAQMSQVTAQIDADFRKTFDDITRQNDDAARKMLQDQMESAHASAQAFQEG
                     SAERVQYLKEYADLAQRYYGSDTIAVVKQAYAEFGAAQRAYNSEQARMNTELYEGWDS
                     LYTQITQRHGQMLEEQLRQSQQAAKEEQRVEQELTDFGESLDKEVLSLKDKNVQEQKA
                     LNDRMAQDFKSTYGGIFNTLESGFTGAISGMLQGTETFSQIARNLFDSLITSVIGMFV
                     KMGVNWAENLAMQHYLTTQHALQTISANAGIAGSAGVASWAGAPWPVDAGAPAFGAAA
                     AADAMSYASFSAAGGFDIPAGLNPVTQLHAGEMVLPQGLADVVRTAASSSGSDGGGGQ
                     QMNVTIQLQAWDGQSVQSWLQSGGAAQISRAVSRYHNVPQPRR"
     gene            complement(4186..4530)
                     /locus_tag="VFB37_07185"
     CDS             complement(4186..4530)
                     /locus_tag="VFB37_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22275.1"
                     /translation="MIRAPFAVHLHQVKERKQPGGRILREPFTVSHFSGEDAKPIELS
                     DAEALAHIHKLEPHDDSAKALFETYHEKQRQAAEARRRQEREMQGPSLSQELALAMVS
                     VLQEAGVIKPAK"
     gene            complement(4571..5776)
                     /locus_tag="VFB37_07190"
     CDS             complement(4571..5776)
                     /locus_tag="VFB37_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HZO22276.1"
                     /translation="MADSRRSEGQGLPTRSVGSVPQRKQPRIDWTPREAAKFSIRKGV
                     LAAITGNWEDAGFEREVSSTLQAAGDYAPSGPGTAFFVPTNFPFAVPGAPFSERSYVV
                     GTASAGGNVVGTELLPADFIEVLRNRTVTGQLGARFLTGLVENVNIPRQNAQTTTFWV
                     GESVALTESEATFDQVQLRPHVVGALSKMSRLMLQQSTPAIEQLVREDLAQVLAITLD
                     AAALFGSGTSSQPTGIANTSGVSQIVGGTNGANFSFDHFIQLYAAPLVANVPQENLAF
                     AVNAKTKSYLATLKSTTGQYLWNPFNSISAVIPDQIVGYRYAVSNQLPYNLTKGTSSG
                     ICSMAAFGNWQELFIGEWGVLEILANPFDSTGFTTGDVLIRAFQTVDVGLRHPASFAI
                     MSDGLTPGF"
     gene            complement(5784..6278)
                     /locus_tag="VFB37_07195"
     CDS             complement(5784..6278)
                     /locus_tag="VFB37_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22277.1"
                     /translation="MNELAQPVSLAKAARELQLHAATLRRWVREGAPVWQEGAAGRGK
                     GALVVVTEIAAWRVQMAARRAVGTEVEFVAPPDGAEALQRKIAQALWRALTRGRHDES
                     EPLHALLGVSSPKAFALCLRSYKEIHQELWGEWPTPNQLPPEMETARACAVLPPSIRQ
                     RFTE"
     gene            complement(6275..6451)
                     /locus_tag="VFB37_07200"
     CDS             complement(6275..6451)
                     /locus_tag="VFB37_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO22278.1"
                     /translation="MKDPRSAAPDLAGRAFIRQHAVAIARLVAAGMSVRQVAEALGLP
                     EALVQRLIRKTGGR"
     gene            complement(6519..7124)
                     /locus_tag="VFB37_07205"
     CDS             complement(6519..7124)
                     /locus_tag="VFB37_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO22279.1"
                     /translation="MLTMTVDEAATAFGVRPKTIREWISEGLPCHRRGDKGLGNGAII
                     DAARAPAWVINRRQLDSSPPWTEHFGDQEQSNPLRMVVEWFADGVPEMITGALLLWLE
                     DGRWRPLGCTEEQAREAAGELYLIMAMALTAYKGQFDTGVSRRMGGADLDDLASLLLR
                     GSVRSDWLSLTVPITERIRPLIPSAIAEKMAARAQRRKSEQ"
     gene            complement(7669..8928)
                     /locus_tag="VFB37_07210"
     CDS             complement(7669..8928)
                     /locus_tag="VFB37_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO22280.1"
                     /translation="MPTIELAPNTDGPPDDGVPLFDYSDEPALHGRANGHLHLAPDTD
                     QDPPAPPVFDPERARLRATILDEAPAPVESIVEGLLRRTAGVRVASGGSGKSTLTLFE
                     MVCVVLGCPLFGREVMRPGPCVLLTAEDERQVVEYRLARILEDMRLPRVDRERVLTNI
                     YVEDLTGRTCRLIDVNLGGSLLPTAAVGELIERYRSIQPAFLSIDPMTLFGPGERHVN
                     DGEAELLRAGRAISYGLRAAVRFEHHSGKQNARDRQTDQYAGRGGSAGADNARFVHVL
                     TVHSPDDKLAAPSRVTPEDIAKGNLLRLHVAKDSYGARLTAPIWIQRSGFLFTHVAPD
                     PQADLDPMQAPLRRLYEFIESEEAAGIRHTANTLDNRLGELQLARQSMRAALHVALER
                     QHLLEQPLPVEEQRGRRKTYLAKGLRP"
     gene            complement(8928..9812)
                     /locus_tag="VFB37_07215"
     CDS             complement(8928..9812)
                     /locus_tag="VFB37_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZO22281.1"
                     /translation="MSTFAELRIAVREPGRVEQRVACPRCAKHDRDDTLGVNTRSGAF
                     HCFRCGWSGRAGTGETQPPVPIACCVDSRQAERKRKRLNDTWASCVPLNDVAAAPVQR
                     YLLSRGLAGLLSQDCPHNLRAHPALKYFDEEGREVGIFAAMVGLLQSLGGRGATLHLT
                     YLNRDGTKAPVSSPKKLLPPAARGATRGGAIQLYAPDKGVLGIAEGIENALSLRSMLG
                     FPCWSTYCAQNLATIALPTGLQLLHIVIDVDASGTGAAAARTLTARAQRQGIDVRHVI
                     PEGAGPRDLNDELRRRIN"
     gene            complement(9809..9979)
                     /locus_tag="VFB37_07220"
     CDS             complement(9809..9979)
                     /locus_tag="VFB37_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22282.1"
                     /translation="MSSPAPRELVHFLLLSPDQQAAAIRRLAASGQSLSTIAAATRLS
                     IEQIQRLLAEET"
     gene            complement(9976..10845)
                     /locus_tag="VFB37_07225"
     CDS             complement(9976..10845)
                     /locus_tag="VFB37_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22283.1"
                     /translation="MHTTRVRGFATYEPRDGAKALIDSTKSVLRQYKDYLPLTLRQIF
                     YRLVTMDVIGKTEKDYKRLGEILNRARRGRLIAFAAIRDDGVVTDAPASYGSVDDALA
                     TLRGHIASLQIDRQAGQPRRMRIWCEAAGMVPQLTRVAHEYGISVMSSGGFDSVTIKH
                     HVGQELAESGRLTVLHLGDLDPSGEHVYSSLDEDVRSFAKSYGGDVDFTRIAVTAYQV
                     HRYGLPTSPPKATDRRSFSGTATTQCEALDPATLAQIVRDAIRCRLDPDAYASALEYE
                     SECRAELTSRLAR"
     gene            complement(11397..11930)
                     /locus_tag="VFB37_07230"
     CDS             complement(11397..11930)
                     /locus_tag="VFB37_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22284.1"
                     /translation="MGRRARQNSRLSRAPAKGVRVPYADLEPGIITLLRAIRGCQHRL
                     RTGDVHPVQAQRLRWALEALDNIVVDWDKTENLNELKTALGVQSNKKRRRNATVRAET
                     NIAVALVNAAVSGNTEPFEAVANQLDLPIERVTRAWRTWQSFYIAQLNISAGKNPEQR
                     RAARAAIDRLKKSKHKL"
     gene            complement(11897..12961)
                     /locus_tag="VFB37_07235"
     CDS             complement(11897..12961)
                     /locus_tag="VFB37_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZO22285.1"
                     /translation="MLTDKAIRALKDPEKDRWHRDGKGLYLRHRTTGGKSWVLRRKKD
                     RGGNITLGAFPDLGCAAARVKAAEFTGKRVTKVTLGELLEDWYTEVITEGYRRPHHVR
                     GYIDRLDSSLKSTKLRDLERLEVRRVARRYAEDKGPVAARRLLSILKTALRFAVDAGY
                     IDVSPIDRLNADLVAPAEATRERVLNDAEIRALWVAESQHTALLRFLLLTGQRIGETQ
                     LATWDDIAGERWSISAAHTKNGRAHWVALSSQARAILDSLSRERRLIFGSATTTGVQA
                     WLRRWCEREGIEPAFRPHDLRRTCATRLNELGVLPHVVEKILNHTMQGVMAVYNRAEY
                     EAERVEAMRKWADELARILG"
     gene            13080..13547
                     /locus_tag="VFB37_07240"
     CDS             13080..13547
                     /locus_tag="VFB37_07240"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HZO22286.1"
                     /translation="MKILFVCLGNICRSPTAAIVLREIAAREAPALPIEVDSAGTADY
                     HVGQPPDRRTREAALRRGYDMSSLRARVVESQDFGRFDLILAMDRANLEVLRRRAPQS
                     AQERIRLFLEFAPEAEAAEIPDPYYGGPNGFEHVLDLVELAARGLLLHLRSAA"
     gene            complement(13579..16140)
                     /locus_tag="VFB37_07245"
     CDS             complement(13579..16140)
                     /locus_tag="VFB37_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZO22287.1"
                     /translation="MKRMLVNATQEEELRVALVDGQKLFDLSIELPSREQKKANVYKG
                     RISRIEPSLEACFVDYGAQRHGFLPLKEVSREYFRQQPQGGRMNIRELLSEGQDLIVQ
                     VEKEERGSKGAALTTFISLAGRFLVLMPNNPRAGGVSRRIEGEDRDQMREVMGQLQIP
                     DGMGAIVRTAGVGRSAEELQWDLDNLKTQWDQIAAAANDRPAPFLVFRESDAVTRAMR
                     DYLSDDIGEVLVDDQGAFQKAQEYMQRFMPADAQRRLKMYQDDIALFTRFQIESQIES
                     AYAHKVQLPSGGSIVIDYTEALVSIDINSARATRGSDIETTALNTNLEAADEIARQLR
                     IRDIGGLIVIDFIDMESTKNQREVEDRLRDAVKMDRARIQIGRLSRFGLLEMSRQRLR
                     PSLGESSHIVCPRCVGIGSIRSIESMALAILRLIGEELRKDRTARVIAQVPVGVATYL
                     FNEKREFLRHLEDKSEVELLIVPNENIQTPEYSIRRVRDDELELPENRQLSYLMPTAP
                     AEAEPGSAKERKPPTESPAVATLLPSTPAPLSAVAAPAAPATPAPAHSGGGFLGWLKG
                     IFGGAPQAAPAAAESAAAPTPTRSTSSGSGGSRDYGHRRRDGRRDHSRHSRHADSGMR
                     RERGDGRDRAEGHDRGETRDRHESRDRNRDQDRRPRERSGERRPEREGSLARTEQNGA
                     RMEQSSHAEESERAGRSGEGAPPPGSELRENGGPDRGERRSRRGRRRGRRGGGGRDSA
                     ARESGSQSASTASADERSAFQGNGTQGYTDTGAHPPEFSQEERHEPSQRSAAEEQAGE
                     QRSPSPEPRPIAHFEPTPPISTSGSESGAERPTKPVVVWSSGPATPTPRDRGPEE"
     gene            16524..17513
                     /locus_tag="VFB37_07250"
     CDS             16524..17513
                     /locus_tag="VFB37_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZO22288.1"
                     /translation="MSYAEVDVSAQQSGEVRQGVRHFEVTENEAGQRLDNFLHKLLGD
                     LPRSRLYRVIRKGEVRVNGHRVGPDTRLQARDKIRIPPVRISATPEPGRPPVALLETL
                     RRAVVHEEADLLVIDKPSGVAAHGGSGISFGAIEALRWLRPDETLELVHRLDRDTSGC
                     LLVARNRGALRTLHALIRHGELEKRYLALLQGKWELGRKRIDVPLRTDARVRGERTVR
                     VDPHGKASVSEFRPVQFFGKRATLVEVTLLTGRTHQIRVHARHAGNPVAGDEKYGDAE
                     FNSALRAFGLSRMFLHAHSVSFQWPGGSEFSVNTPLAPELASVIDALGTRPRR"
     gene            complement(17510..18085)
                     /locus_tag="VFB37_07255"
     CDS             complement(17510..18085)
                     /locus_tag="VFB37_07255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZO22289.1"
                     /translation="MPALILASSSPYRRSLLQRLRLSFDTVPPSSSEAELEGELPPDR
                     AVRLSLAKAQVVAARHGGAVVIGSDQVAAAGSTVLDKPGDAERCRAQLRQLSGATALF
                     HTGCAVLGPEGQRLVHLDTTRVRFRPLRAAEIDRYIRLEQPFDCAGGFKAEALGITLF
                     ETVETKDPTALIGLPLIWLAEALRKLGFDLP"
     gene            18194..18709
                     /locus_tag="VFB37_07260"
     CDS             18194..18709
                     /locus_tag="VFB37_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HZO22290.1"
                     /translation="MSPPWSKPLDIDRLADSEADVAFVVSLAELPRLRSRLEGVSGTV
                     AGHVRFSREAGLVVADVEVSGDATLICQRCLQPMSEPVNSSVRVALIATEAALARVPD
                     QLEPVLAAGGRISIGELVEEELLLALPIVPLHAPAAACIVAKDAPLVSEDKPDHTTQR
                     PFERLSELLKR"
     gene            18727..18921
                     /gene="rpmF"
                     /locus_tag="VFB37_07265"
     CDS             18727..18921
                     /gene="rpmF"
                     /locus_tag="VFB37_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZO22291.1"
                     /translation="MPVQKSRKTPSRRGMHRSHDGLDKPALSTDPQSGETHLRHRITP
                     DGFYRGRRVIEKPADADDKE"
     gene            18963..19985
                     /gene="plsX"
                     /locus_tag="VFB37_07270"
     CDS             18963..19985
                     /gene="plsX"
                     /locus_tag="VFB37_07270"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZO22292.1"
                     /translation="MLEIAIDVMSGDHEPREYVAGSLHALADDADLRALLVGRPEAIQ
                     PLLAHESASLRERIEIVPASQVVAMGDSPREAIRRKKESSMRLAIDLVKAGRAGACVS
                     AGNTGALTAMAHFVLKTVAGVERAAIISAIPATHGHTQMLDLGANTKATPEQLLQFAA
                     MGAIIARDIHGLRDPRIGLLNIGEEDIKGHEIVQAAHALLGQSGLHYVGFVEGDDIFF
                     GDVDVVVTDGFTGNVALKTMEGAARLVANRLRAEFHASWPARLTGWMARPVLRRVAAG
                     LDPNRYNGACMVGLAGIVVKSHGRADGVAFSRAIATAALAARRGLTAHIEQALRDNNS
                     VRAQGT"
     gene            19996..20958
                     /locus_tag="VFB37_07275"
     CDS             19996..20958
                     /locus_tag="VFB37_07275"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZO22293.1"
                     /translation="MIHSRITGTGSHLPEKILTNADLEKLIETNDQWIRERTGIERRH
                     IAADGETTVDLAERAARRALDAAGVRPQDVDFIAFGTTTPDLVFPNCGVLLQGRLGCR
                     GGPAFSVETACSGFMYALSIADKYVRCGESKRALVVGSETLSRITDWTDRSTAILFAD
                     GAGAVLLEPSEQPGIVSTHLHADGAYKDLLYCGGGVSKGFDPAGRLAISMVGSEVFRV
                     AITTLGRAVDETLEANNLGRSAIDWLVPHQANLRIIQAMARKLDLPMERVIVTIQDHG
                     NTSAASVPLALDFAIRDGRIKRGDLLLLEAFGGGFTWGSALVRY"
     gene            complement(21283..21597)
                     /locus_tag="VFB37_07280"
     CDS             complement(21283..21597)
                     /locus_tag="VFB37_07280"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HZO22294.1"
                     /translation="MKCAIVLKVAAVAVVAGLAGCQDLKPLQADVADLKSQVAKLQSD
                     VQAAKSTADQANSTAQSASQSASSAQSTANQALAAAQAAQSAVDATNEKIDRMFKRSI
                     SK"
     gene            22113..22736
                     /gene="lexA"
                     /locus_tag="VFB37_07285"
     CDS             22113..22736
                     /gene="lexA"
                     /locus_tag="VFB37_07285"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZO22295.1"
                     /translation="MSDLTPRQTQILRLVQRFISETGMPPTRAEIARELGFRSANAAE
                     EHLRALARKGVIALVPGTSRGIQLKDTIREQMGLPLIGRVAAGKPIFAEENFEMRLQV
                     DPGLFQPRPHYLLKVTGMSMKDAGILDGDLVAVHRTSEVRSRQIVVARLENEVTVKRY
                     RQEGSVVWLLPENADFEPIKVDLKQDPLLIEGIVVGVLRRGKTTGLM"
     gene            22794..23489
                     /locus_tag="VFB37_07290"
     CDS             22794..23489
                     /locus_tag="VFB37_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22296.1"
                     /translation="MPAADELSRLLEHPAIWRGVSAARMEVLPTGFSALDERLPGGGW
                     PARGLIEILVSRFGIGELSLLLPVLATLTRRPMARWCVWTAPAFPGSALPSDGNESCL
                     LEPFAPALVAHGIALERVLVVRVPASSPRALSSQTLWAFEQSLGSGACDVVMAWARRP
                     RAREIRRLQLAAERGRALGVLVRPQRAASEASYAILRLKLEPATQGVRVTILKSRGGK
                     RGSVELALHASGN"
     gene            23633..24910
                     /locus_tag="VFB37_07295"
     CDS             23633..24910
                     /locus_tag="VFB37_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZO22297.1"
                     /translation="MTSSPSNPGESTSSPPLEQLATRAQRFTPRVSLEPPDGLLLEVK
                     GSLHLFKGAAGLMQALGEEYGDIGKVSSFALAPTPLAALLMARWGKPLIITDKAQLIG
                     HLAPLPLKLMRWRADVPERLARMGVRTVGEALRLPRAGFARRFGIAPLADLDRLTGRA
                     ADLPRPFFARERFRRRRELTYELENTERILIVLAPLLTALGDFLRLRQCGILKLECLL
                     RHRHAPASSCVLRLAAPLADVSRLTELLGERLSALELPEPVRACELRSGTLVPYLPAS
                     RSLWQPGEQGGGETQSNELIEHLRARLGATAVCGLQVLAGHRPETASSVREPNAGFAA
                     VDGSPPPPWSAFRRPLWLLPAPQLLQQKEGRPRRRGPLRLAAEMERIESGWWDGTDIE
                     RDYYIARDSYGVELWVFRERTAPHRWFLHGVFG"
     gene            24907..28095
                     /locus_tag="VFB37_07300"
     CDS             24907..28095
                     /locus_tag="VFB37_07300"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZO22298.1"
                     /translation="MNCESSCAAPPRQIPYAELHCLSNFTFLRGASHPQELIERAIEL
                     GYMALAITDECSMAGVVRAHVAARDTALKLLIGAEFRLECGLKLVAIAMDRAGYGRLC
                     RLITRGRCAAVKGKYCLKREDVEASGLQQCFVLWTPCARRATDPRTLDESRWQDEARW
                     LSQQFGDRARIAVELFREGTDRQHLLGLQSLGREWGLRLVASGDVHMHGRERRRLQDA
                     LTAVRLNVPIAQAGWHLYPNGERHLRDYERLVRIYPQELLAESITIAQACTFSLDELR
                     YEYPHELVPAGETPSSHLRKLVEKGVRKRWPEGVPGNVRTTLESELALIEELRYEPYF
                     LTVQDIVAYARANGILCQGRGSAANSLVCYSLEVSAVGPERGAALLFERFISRERNEP
                     PDIDIDFEHDRREEVIQYIYRKYGRDRAAIAATVIMYRPRSALRDMARIFGLDSASSA
                     RLSQVMQWWDGNETLPARVREAGFDMQDPILQRIVSLARQLVAFPGFPRHLSQHVGGF
                     VISEGLLEELVPIENATMPERTVVQWDKDDLNDLGLLKVDVLALGMLSALRRAFALVN
                     DYRGTRHVLGGLPDRDAEVYAMMSRADTVGVFQIESRAQMSMLPRLKPNCYYDLVIEV
                     AIVRPGPIQGDMVHPYLQCRAKNEPIEYPSDAVAEVLRRTLGVPIFQEQVMQLAIVAA
                     GFSPGEADRLRRAMGAWKKSGGIEHFRDKLLGGMQARGYEEEFAQRLYRQMLGFGEFG
                     FPESHAASFALLVYDSAWLKLHEPAAFTCALLNSQPMGFYAPAQLVRDARGHGVEVWP
                     VDVQESAYDCTLARRDDGEPALRLGMRMVKSLSQAGAMRVEAARPAGPFTSVQDLARR
                     AALDRGDLEALAAAGALAALSGNRHLAYWEVAGAERPLPLAAQASSGHPGSEPLGNLQ
                     EGRPLLAAPTEGQGIVADYASVGLTLERHPLALLRERLSHDRILRMDELDRIAHGRIV
                     RTAGIVLMRQRPGSANGVTFVTIEDESGQVNVIVWENVGRQQRRAMLESRLLEVHGEL
                     QYQEGVRHLIARRLVDRSALLGELLTRSRDFH"
     gene            complement(28157..28444)
                     /locus_tag="VFB37_07305"
     CDS             complement(28157..28444)
                     /locus_tag="VFB37_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22299.1"
                     /translation="MSEKSESEEFEDEEADEPVVGDADVDLDQVIKDLDLAKRRGQKA
                     GDPAWRRLERLLEDKRTAELTSDFDDYDIGVDDSEEESTEPASARVRRKPT"
     gene            complement(28523..29737)
                     /gene="gspF"
                     /locus_tag="VFB37_07310"
     CDS             complement(28523..29737)
                     /gene="gspF"
                     /locus_tag="VFB37_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HZO22300.1"
                     /translation="MGAFEYTALDTGGKERKGILEGDTPRHIRQLLREKHLLPVTVSE
                     VAQKEARRQRSFTLTRGVSTGDLSLFTRQLATLVRAGLPLEEALLAVSQQTEKPRVQS
                     IVLGVRSKVMEGHTLADGLADFPRVFPEIYRSTVAAGEQSGKLDGILERLADYTEGRE
                     LLRQKVLAAMLYPIVLSVMCFAIVSGLMVYVVPKVVAVFEQAKGKLPLITKILIATSG
                     FVRDWGIWMVVGAVIAVFLFSRWLRNPGNRRRYHRVLLRLPMVGKLTRGFNTARFTRT
                     FSILSASSVPVLEALRISGEVVTSLPMRDAVSEAAARVREGAPIGRSLGMSRMFPPMT
                     IHLISSGESSGELEGMLERAAISQERELDSLLAGMVGLLGPMLIVFMGLFVMGIVFAM
                     LLPIFEMNQLIK"
     gene            complement(29744..31210)
                     /gene="gspE"
                     /locus_tag="VFB37_07315"
     CDS             complement(29744..31210)
                     /gene="gspE"
                     /locus_tag="VFB37_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HZO22301.1"
                     /translation="MTHERRLSFAFAKRHGVLVKRLVEGGAECACRENVSPLAIAEVR
                     RYLRLPMRIERVPEGEFDILLRQAYEAGSDTMQAVEGLDDTTDLAHLAQDLPEQADLL
                     DSDDDAPIIRLINAVLTQAVRENASDIHVEPFENRLVIRFRVDGILREVLQSKRAVAP
                     LVVSRLKVMSKLDIAEKRLPQDGRISLKIAGRSVDVRVSTIPSGHGERVVLRILDKQA
                     GRLDLNALGMVPEIAALIDDLIHKPHGILLVTGPTGSGKTTTLYAALERLNDNTRNIM
                     TVEDPIEYYIDGIGQTQVNTKVEMTFARGLRAILRQDPDVVMVGEIRDLETAQIAVQA
                     SLTGHLVLSTLHTNTAAGALTRLRDMGIEPFLLSSSLVGVLAQRLVRVLSPDTKQAFQ
                     AGEYERRLLNLRPEDPSPVLYRPGRDVAGGYRGRSGIYELIVVDDQMRTMIHDGASEQ
                     ELERYARHTTPSIREDGRAKVLRGETTIEEVLRVTRED"
     gene            complement(31228..>32049)
                     /locus_tag="VFB37_07320"
     CDS             complement(31228..>32049)
                     /locus_tag="VFB37_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22302.1"
                     /translation="PFVTGSFANTGTVSNGAVNPFQTIQREEVGTILKVTPNIAAEGN
                     SVVLKISIESSSVLPTSVSSVDITTSKRTITTNVLIEDGGIVVLGGLISDSNSKDSQQ
                     VPFLGSIPIIGQFFKTRHTEATKNNLMIFMRPKILRDQTQAAYEADSKYNYMIEQEKT
                     YNGKREWLPLLPGVNKPLLKSPPPPPPPGSTPVAPASPEEKEKAAKAQAAADAEAAKK
                     LSKPGAITQPIAPPKPPQSDGSAASASPDLGVTPPPPRTAPDTSPEATPQPEPKQ"
BASE COUNT         5443 a        10173 c        10396 g         6037 t
ORIGIN      
        1 gcctgcgcag cgtgtcagac ggcccccgcg accgaagccc ggcctggtca tcgccgagtc
       61 cgccgaacac gagggcttcc ggacccgaga cgtgttggct gcgctgcggg gcgtcacgct
      121 cgcgctcgat atgcactttc agagcctgga catcgagccc catgaactcg ccacggcggc
      181 gcgggtgctc gcagcgattc tctacaaccg ggccttctga ggaagggatc gagaacgaca
      241 ttgggggccg cctgctcgcg gggggcggcc ctttttttgg gcgcagccgc gggggcccgc
      301 gcccgcgcgt tatcccccag tgtggtctct gcgcacttgg tcaacgatcg ccctgaacgc
      361 ggaggtatcc tgccaaattc tctcatcggt tcgtcgcagc ttcatctcca ccaggtccgc
      421 gagcactcgc ttgagcggct caacgaaata acacaccacc tccgacctgt tggctgctac
      481 ataggtggct cgctgcgcat tttcgttgta gaaaaggcaa tgcttcagag aagagtcggt
      541 cgcggtcttc aggaactcat ccaactcgct cgggatgcgt acatgtaagc ggatcctagc
      601 gacttgctcc gcatcatcgc gcgcagcgcg cagacgatcg aagtcctcgc gatgaaattc
      661 ccacgccgaa tcccgcttgc gataccaaga ccccccgacg tcactgaatg tggcggagac
      721 cgcgccctcg gggaaaggcg ctgcgcgggc cgcctccgcg ctcgcgaaca gcttgtcgaa
      781 aatcgtgccc ggctgaccat cgggcgcttt gacaagcttc ccctctgaag catagaagac
      841 cttgacagtc atttccgttt ctcatcctct cagattggaa tcggccggca ggcccaatac
      901 tgcgtccaag cgtccgaata tctcagtcac cgcgtgacca gaagccgcag ccatgaacgc
      961 ctgtgaagac agtcccacgt gatccaccac gcgccccacc gccggccggc aagtcgcacg
     1021 atgcaggagc tcggcccctg cgccgagtgt cgcttcagcg cccgctgcgc ggcggagcgc
     1081 ttggcgtgcg cagccttcgt gatgttcagc cgtggcaaag gggcgccccg ctggcgaagc
     1141 ggctcctcga gcccctacgg cagcgatatg gcaggcgctc ttcgattaag acggcatcga
     1201 tgaatgtgtt gcgagcgcct cgagggcggc cgccgcgcag gggcaggcgg gctcgagccg
     1261 tgcgagatcc gcggcagctt cttcatcgct gagtgtgacg acctggccgg cgggtataca
     1321 ccgtgccgtc tttcgtcacg ctgaagtctg ctcgcacgcg atactggcgc tgggtcatgg
     1381 cggggtctcc tgaaggggcc gatgattcac tggtcagcct gatatcgcgc gagcacatct
     1441 cgctgcgccg cgtgaatgcc ggccaaggcc aggtgcccga gctggtagat gagcacctca
     1501 tacataactt gcatctgctc gccgagctct cgagccgttc cgccacgggc ggccaccgct
     1561 tgcagctcgc cgatctgctt gcgtagctca tcgtggccgg cgagtacctc gtctgccagc
     1621 caggtggcgg cgtcctcgcc cttgggtcct gtcagtaaga gcatggcgct gccgccaaac
     1681 tcggcccgat ggcgggcccg gatcgcggcg aggtcctggt gcgtggcgct catcggcgcg
     1741 gctgcggaac gttgtggtaa cggctcactg cgcgcgagat ctgcgccgcc ccaccgctct
     1801 gcagccagga ctgcaccgac tgcccgtccc aggcctgtag ttgtatcgtg acgttcatct
     1861 gctgcccgcc tccaccatcg gaccctgacg agctggccgc ggtgcgcacc acgtccgcga
     1921 gcccctgggg caataccatt tcgccggcgt gcagctgcgt caccgggttc aggccagccg
     1981 ggatatcaaa gccgcccgcg gcactgaaag aggcgtacga catggcatcg gccgcagcgg
     2041 cggcaccgaa ggccggtgca cccgcatcga ccggccacgg cgcgcccgcc cagctcgcca
     2101 ccccggctga tcctgcgatg ccggcatttg ctgagattgt ctgcagtgca tgctgagtcg
     2161 tcaggtagtg ctgcatcgcg aggttttcgg cccagttcac gcccatcttc acaaacatgc
     2221 cgatcacgga ggtaatcaac gaatcaaaca ggttcctcgc gatctggctg aatgtctctg
     2281 ttccctgcag catgccgctg atcgcaccgg tgaaccctga ctccagcgtg ttgaaaatcc
     2341 cgccgtaggt ggatttgaag tcctgggcca ttcgatcatt gagcgccttt tgctcctgga
     2401 cgttcttgtc cttgagcgag agcacctcct tatcgaggct ctcgccaaag tcggtgagct
     2461 cttgctcgac ccgctgctct tccttggccg cttgctggct ctggcgcagc tgttcctcga
     2521 gcatttgccc atggcgctgt gtgatctggg tataaagcga atcccaacct tcgtagagct
     2581 ccgtgttcat gcgcgcctgc tcggagttgt aagcgcgttg ggcggccccg aactccgcgt
     2641 aggcctgctt cacgaccgcg atcgtgtcgc taccgtagta ccgctgcgcg agatcggcat
     2701 actccttcag gtactgcacg cgctcggcgg aaccttcctg aaaggcctgc gcgctggcgt
     2761 gcgcactctc catctgatcc tggagcattt tccgggcggc atcgtcgttc tgccgcgtga
     2821 tgtcatcgaa ggtcttgcgg aaatcggcat cgatctgtgc cgtaacctgg ctcatctgcg
     2881 cgcccatctg cgcgaacgga ttggtgccta ccgcgccccc aagcgtggcc ttgctcagct
     2941 gcgcggtgga cgcggcaccc tgcagtgaat tgaaaccatc gatcgcgccc aaaactgcct
     3001 ttttgaaggt gttgctgagc gagtcggaca gctcgtccca acggcgcttg agatcgacca
     3061 gggcggtgtt ctgctccccg gtgagcgcat tgactcgctc gttagcgctc gccactccct
     3121 gtgtcgatcc gacgtagtgt tccagcgccg cgatcataag cgccgagcgc gagccgtaga
     3181 tttgcagcaa ggcatcgtgc tcggcctgcg ccgtggaggc attagtcagg cggtcgtgca
     3241 gcaactgcaa caggtcgttt agctgaaatg tggggctttg gatctgttcg atcgacaccc
     3301 cgagcgcgag cattttgtcg atcgcctgct gcgagccggc gcgcgcctcg tccagagtct
     3361 gcgcgaggcg ttcgctcccg cgagccagga cctccggccc cgcgccagcc tcctcgccgg
     3421 cgagccgcag cgcctggaac tgttccacgg tgacgcccag cacgttggac aacgtgctga
     3481 tctggatggc ttcggatccc atcgagttga tgatctcgga gaccttcgcc agcccggccg
     3541 tggcggccgc aagtccggtg aacgcgaagg cggcgctgaa cttcgactgg atatcctcga
     3601 gcgcaccacc gaggcccgcc gtctgatcgg tgatcgactg gaagaagctc tgcgcggcac
     3661 ccttcgcctc ctcgagggag gcgaggaatt gcgagttgtc ggcagtgatg ttgcttgaga
     3721 tgtcttgcga catcagtgcg ccctcccgac gagctcgctg ctgagctccc gcaggtgagc
     3781 ctcgccggcg ctgaactctc tcaaggcggt gcgagcgacg gcattggctt cggagatcgc
     3841 agcttcgtct gtcggcgcaa cgaccgtgaa cgaaaagcgc gctgtttgac tgcctctcac
     3901 aattgaaagt gcgtaccggt gcctgagcat gtctcggtcc tccgaagggg ccccgtgcgg
     3961 cggtcccaat gtgccgccgt ccgaggagcg ctcacgaatg tcctcgaggg cacactcgcc
     4021 ggatgcccat gtctcgccca ggcttcgcgg cggcgcccaa cgtcggggag tgcagcaccg
     4081 agggatgccg cgcaacgtct gaaccgctgc cggtgtttct ttcgataccc gcgggcgact
     4141 gaccggctaa aacccgccct cgcggcgcac gcgaggccga acatttcatt tcgccggttt
     4201 gatgaccccg gcctcctgca atacgctaac catggcgagc gccaactcct gtgagaggct
     4261 gggtccttgc atctcgcgtt cctggcgccg gcgggcctcg gcagcttgac gctgtttctc
     4321 gtgataggtt tcgaatagcg ccttcgccga atcgtcatgc ggttcgagtt tgtgaatgtg
     4381 cgcgagcgct tccgcgtcgc tcagttcgat gggcttcgcg tcctcgccgg agaaatgcga
     4441 taccgtaaac ggctcgcgca ggatgcgccc accaggctgc ttgcgctctt tgacctggtg
     4501 cagatgtaca gcaaacgggg cgcggatcat gtacgtactc acggtaacag tctccttcgc
     4561 gaatctgatg ttagaagccg ggcgtgagtc catctgacat gatggcgaag ctcgccgggt
     4621 gccgcagacc gacatcgaca gtctgaaagg cacggatcag cacatcaccc gtggtgaagc
     4681 ccgtcgagtc gaaggggttc gccaggattt ccaacacgcc ccattcgccg atgaacagct
     4741 cctgccaatt tccaaaagcc gccatcgagc agatgcccga gctcgtgccc ttggtcaagt
     4801 tgtagggcag ctgattcgag accgcatagc ggtagcccac gatttggtcc gggattactg
     4861 cactgatgct gttgaagggg ttccacaggt actgacccgt ggtgctcttc agtgttgcca
     4921 ggtaagactt cgttttcgca tttactgcga aagccagatt ctcctgtggg acattcgcga
     4981 ccagtggtgc ggcgtaaagt tgaatgaagt ggtcgaagct gaagtttgcg ccattcgtac
     5041 cgccaactat ctgagagacg ccgctggtgt tggcgatccc agtcggttgc gagctcgttc
     5101 ccgagccaaa caatgcggct gcgtcgagcg tgattgccag cacctgggcg aggtcctcgc
     5161 gcaccagttg ctcgatcgcc ggcgtggact gctggagcat caaacggctc atcttggaga
     5221 gcgcgccgac cacgtgcggc cgcagctgca cctggtcaaa cgtggcttcc gattccgtga
     5281 gcgccaccga ctcacccacc cagaaggtgg tcgtctgggc gttctggcgc gggatgttga
     5341 cgttctccac cagtccggtg aggaagcgtg cacccagctg cccggtcacg gtgcgattgc
     5401 gcagcacctc gatgaagtcg gccgggagga gctcggtgcc cacgacgttg ccgcctgcgg
     5461 aagcggtccc caccacatag gagcgctccg agaagggcgc gccgggtacg gcgaacggga
     5521 agtttgtcgg cacgaagaag gctgtgccgg gccctgatgg cgcgtaatcg cccgccgctt
     5581 gcagagtgct gctcacctcg cgctcgaaac cggcgtcctc ccagttgccg gtgatcgccg
     5641 caaggactcc cttacgaatg gagaacttcg ctgcctcgcg cggggtccag tcgatccgcg
     5701 gctgctttcg ctgcgggacg ctgcctacgc tgcgcgttgg gaggccctga ccctctgagc
     5761 gtcgactgtc tgccaccttg tgttcactcc gtaaatcttt ggcggatgga tggtggcaaa
     5821 actgcgcacg cgcgcgcggt ttccatctcc ggcggcagct ggttgggggt cggccactcc
     5881 ccccacagtt cctggtggat ttccttgtac gacctcaagc acagtgcgaa cgctttcggg
     5941 gagctgacgc cgagcagcgc gtgcaacggt tcgctttcgt cgtgtcgacc tcgagtaaga
     6001 gccctccaga gcgcttgagc aatctttcgc tgcagtgcct cggctccgtc aggcggtgcg
     6061 acgaattcca cctctgtgcc gacggcgcgt cgcgctgcca tctgcacgcg ccatgccgct
     6121 atctcggtca ctacaaccag cgcgcccttg ccgcggcccg ctgcgccttc ctgccagacc
     6181 ggagcacctt cccggaccca tcgccgcagt gtggccgcgt gcagctgaag ttcccgcgct
     6241 gccttcgcga gtgaaaccgg ctgcgcaagc tcgttcatcg gccacctgtc ttgcggatca
     6301 ggcgttgcac gagagcctcc ggcagcccaa gggcctccgc gacttgccgc accgacatgc
     6361 cggctgcgac tagccgcgcg atcgctaccg cgtgctggcg gatgaacgcc cggccggcga
     6421 ggtcaggagc ggccgagcgc ggatccttca tataggcagg cttccaaaag tgacacgcgt
     6481 gtcacttctc cggatgcgcc cgcaagctgg gccgacgttc actgctcgct cttccggcgc
     6541 tgcgcgcgag cggccatttt ctcggcgatg gccgatggga tgagcggccg gatgcgctca
     6601 gtgatcggca ctgtgagaga gagccagtcg cttcgcaccg aacccctcaa cagcagcgac
     6661 gccaggtcat caaggtccgc accgcccatc cgcctggata ctccggtgtc gaattgcccc
     6721 ttgtacgccg tcagagccat agccatgatc agatacaact cgccagccgc ctcgcgcgcc
     6781 tgttcctcag tgcacccaag cggccgccag cgcccgtcct cgagccacaa cagcagcgcg
     6841 ccggtgatca tctcgggcac tccgtcggcg aaccactcca ccaccatgcg cagcgggttg
     6901 gattgctcct ggtcaccgaa gtgctccgtc cacgggggcg agctatcgag ctgtcggcga
     6961 ttgatgaccc acgccggcgc gcgcgctgcg tcaatgatgg cgccgtttcc gagcccttta
     7021 tcgccacgcc gatggcaagg cagcccctcg ctgatccact cgcggatggt cttcggcctg
     7081 acgccgaacg ctgtagcggc ctcgtccact gtcattgtca acataaacgc acctacttcg
     7141 aaaagtttgg gacatccaaa aatggcgcgg gcgcaacgac ccgtgctgct gcaccgcaga
     7201 agaacctaag atttcacagc cggcccggtc agtccggttt cggctcgcgg cggcgcgcgt
     7261 cttctgcctg gtgctcacgt ccgctttcgc tgcactcggt catggtcagc cgcgagccgc
     7321 cggtcccgcg gtgacggatt cgaccgcggc gggtgttgcc ggtagtgctc acgccatcgt
     7381 cgcgcctgcc ggcgtgccca gcgcctgcta atgctcacgc tcggctgccc ttgccgtcgg
     7441 acgtgcagac gtctgcacga gcgcctagct ggcctggtct caggctctga ccggcggcgg
     7501 aattacggcg gattggtcgc catttcgggg tcgaatcgag cttctccact aatccgccgc
     7561 cgcctatagg gaaagaagaa ttggcggaat agatcgccat attctctttc ccacctttct
     7621 ggcaactcgg cggcatgctg gcggatatgc ggcggattgg cggattagtc acggcctcaa
     7681 gcccttcgcc aggtaggtct tgcgacgtcc gcgctgctct tccaccggca agggctgctc
     7741 gaggagatgc tgccgctcga gggccacgtg cagggcagcg cgcatgctct gtcgggccag
     7801 ctgcagctcg ccgagccggt tgtcgagggt attggccgtg tgtcggatac cggcggcctc
     7861 ttcgctctcg atgaactcgt agaggcggcg cagcggcgcc tgcatgggat cgaggtcggc
     7921 ctgcggatcg ggcgccacgt gcgtgaatag gaaccctgag cgctggatcc agatcggtgc
     7981 cgtcagccgc gcgccatacg agtctttcgc cacgtgcagc cgcaggagat tgcccttggc
     8041 gatatcttct ggcgtgaccc gactcggtgc cgcaagcttg tcatcgggac tgtgaacggt
     8101 gagcacgtgc acgaagcgcg cattgtcggc ccctgcgctg ccgccgcggc cggcgtactg
     8161 gtctgtctgg cgatcgcgcg cgttctgctt gccagagtga tgctcgaatc gaaccgcggc
     8221 gcgcaagcca tacgagatag cgcgaccggc gcgcagcagt tccgcctcgc cgtcgttgac
     8281 gtgacgctcg ccgggcccga agagcgtcat cggatcgatg ctgagaaaag ccggttggat
     8341 ggaccggtag cgctcgatga gctcgccgac tgcggccgtc ggtagcaatg agccacccag
     8401 gttcacatcg atcaatcgac acgtccgacc ggtcaggtcc tcgacgtaga tgtttgtaag
     8461 cacacgctcg cggtcgacac gtggcagtcg catgtcctcg agaatgcgcg caaggcgata
     8521 ctcgactacc tggcgctcat cctcggcggt gagcagcacg cacggaccag gacgcatcac
     8581 ttcgcgaccg aacagcggac agcccaacac gacacagacc atctcaaata aggtcagcgt
     8641 tgacttgccg ctaccaccgc tcgccacgcg cacgcccgca gttcgcctga gaagcccttc
     8701 cacgatcgat tcgacgggag ccggagcctc atcaagaatg gtcgcgcgca gtcgtgcgcg
     8761 ctccggatcg aacaccggcg gggctggggg atcctgatca gtgtcgggcg cgagatgcag
     8821 atgaccgttt gcacgaccgt ggagcgccgg ctcgtccgag tagtcgaaga gcgggacgcc
     8881 atcatcgggc ggtccatcag tattcggggc aagttcgata gtgggcatca gttaatccgc
     8941 cgccgcagct catcattcaa atcacgcggg ccggcgccct cgggaatcac gtgacggacg
     9001 tcgattccct gccgttgcgc gcgcgcggta agcgtgcgag ctgctgccgc cccggtgccg
     9061 gaagcatcga cgtcaatcac gatatgcagc aattgcagtc ccgtgggcag cgcgatggtc
     9121 gccaggtttt gggcgcagta cgtggaccag cagggaaatc ccaacatgga gcgcaagctc
     9181 agcgcgttct cgataccttc ggcgatgccc agcacgccct tatccggcgc atagagttga
     9241 atcgcgccgc cgcgcgtcgc gcctcgagcc gcaggcggga gtagcttctt cggactcgag
     9301 acgggcgctt tcgtgccgtc tcgattcaag taggtcagat gtaacgtcgc gccacggccg
     9361 cccaggctct gcagcaggcc aaccattgcg gcaaagatcc cgacctcgcg gccttcctca
     9421 tcgaaatact taagtgccgg atgcgcgcgc aggttgtgcg ggcaatcctg actcaatagg
     9481 cctgcgaggc cgcggctgag cagatagcgc tgtacgggtg cggccgccac gtcattgagc
     9541 ggaacgcaac tagcccaggt atcgttcagc cgtttgcgct tgcgctccgc ctggcgtgaa
     9601 tctacgcagc aggcgattgg caccggcggc tgtgtctcac ccgtgccagc tcggcccgac
     9661 cagccgcagc ggaagcagtg gaaggcaccg ctccgcgtgt tgacccccag agtgtcatcg
     9721 cgatcgtgtt ttgcacagcg cgggcaggcg acacgctgtt ccacccggcc cggttcacgt
     9781 acagcaatcc tcaactcagc gaaggtgctc atgtctcctc cgccaggagg cgctggatct
     9841 gctcaatcga gagccgtgtg gccgccgcaa tcgtgctgag gctctgtccg ctcgcagcca
     9901 ggcggcgtat ggcggctgcc tgttggtccg gactcagcag cagaaaatgc acgagctccc
     9961 gcggggcggg cgagctcatc gcgccagcct cgaggtgagt tccgcgcgac attcgctctc
    10021 gtactctagc gcggaggcat acgcgtcggg gtccagccga cagcgaatcg catcacggac
    10081 aatctgcgcc agcgtggcgg gatcaagtgc ctcgcattgc gtcgtggcag tgccggagaa
    10141 actccttcgg tcagttgcct tcggaggtga agttggcagc ccataccgat gcacctggta
    10201 cgcggtgact gcgatgcggg tgaaatcaac atcgccgccg tagctcttcg cgaaggatcg
    10261 gacatcctca tcaagagagc tgtagacatg ctcgccgctg ggatcaagat cgccaagatg
    10321 caataccgtg agtcgtccac tctcggcgag ttcctggccc acgtggtgct tgattgtcac
    10381 cgagtcgaag ccccccgagc tcatcacgga aatgccgtac tcgtgggcaa cgcgggtgag
    10441 ctgcgggacc atgccagccg cctcgcacca gatgcgcatc cggcgcggtt ggccggcttg
    10501 tcgatcgatt tgcaaagacg ctatgtgacc acggagcgtc gccaaggcgt catcgacgct
    10561 gccgtaagac gcaggagcat cggtaacaac gccatcatcg cgaatcgcag caaaggcgat
    10621 cagccgtccc cgccgcgccc ggttcagaat ctcgcctagg cgtttgtagt ccttctccgt
    10681 tttgccgatg acatccatcg tcaccagccg gtagaagatt tgccgcagcg tcagcggcag
    10741 gtagtccttg tactgacgca gcacagactt tgtggagtcg atcagcgcct tggcgccatc
    10801 gcgaggctcg taggtagcaa aaccgcgcac gcgcgtggtg tgcatgaccg cgctcaccgc
    10861 cgcaccgccc gccggaattc ctccagcacg cgccagagtt ggtgatatgc gccgcagagc
    10921 tcgcagagct ccgcaaatcg gtcagctgtt ggggcgccgc agcctcgaca agggcgcgtg
    10981 cgcggcttac gccaatgcgg tagagtctcc atcgattgag gatctcctgg tgaattgaac
    11041 gcccgcgcgc tgtggtacgg ccgcgggcgt tttcttgaaa gtcaacattt agggcgcgcg
    11101 ggcgccggct cgagcagctg cggccgtttt ggaatagtca gtttttggct gagacgccgc
    11161 agcgtatcga tcgatctccg ctgcggtgag aaacggcgta ccaccgtcat acgtgacgtt
    11221 gagccggcct tccttgacgc gctgatagcc ctttgcgcgg ctcagcccca gaaggacgag
    11281 tccctcgccg aggcggtatc gaggcttggc gtgaatcatt gaatcagccc tcgggcgtcc
    11341 acaatggaca gggcgagacg ctacaggcga cgaacgcgtg tacgcaaaac aggaactcag
    11401 agtttatgtt ttgacttttt gagcctatct attgcagcgc gtgcagcacg tcgctgttcc
    11461 gggttcttcc cggcgctgat attgagttga gcgatgtaga aggattgcca ggtcctccag
    11521 gcgcgggtca cgcgctcgat tggcagatcg agttggttgg cgacagcctc gaaaggctcc
    11581 gtattcccgg aaaccgcggc attgacgaga gccactgcaa tgttagtttc cgcgcgcacg
    11641 gtcgcgttgc gccggcgttt cttgttcgat tgcacgccga gtgcagtctt cagctcgttc
    11701 aggttttcgg ttttgtccca gtcgaccacg atattgtcga gcgcctcgag cgcccaccga
    11761 agccgctgag cctgaacggg atgcacgtcc ccggtgcgca agcgatgctg acagccgcga
    11821 atggcgcgca gcagcgtgat gatcccgggc tccaggtccg cgtagggtac ccggacgcct
    11881 ttcgccggcg ctcgactcag ccgagaattc tggcgagctc gtcggcccat ttccgcatcg
    11941 cctccacgcg ctccgcttcg tactccgcgc ggttgtagac agccatgacc ccttgcatgg
    12001 tgtgattgag gatcttctcg acgacgtgcg gcagcacgcc cagctcgttc agccgtgttg
    12061 cgcaggttcg gcgtagatcg tgcggtcgga acgcgggttc gataccttcc cgctcgcacc
    12121 agcgccgcag ccaggcctgc acgcccgtcg tcgtggcgct gccgaagatc agtcggcgct
    12181 cgcggctcag actgtcgaga attgcccgcg cctgacttga tagcgcaacc cagtgtgcgc
    12241 ggccgttctt ggtgtgcgca gcggaaatac tccagcgctc gccggcgata tcgtcccagg
    12301 tcgcgagctg ggtctcgccg atgcgctggc ccgtgagcaa caggaagcgg aggagggcgg
    12361 tatgttgaga ttccgccacc cacagcgcgc ggatctcagc atcgttcagt acgcgctcac
    12421 gcgtggcctc cgccggcgcc acaagatccg cattcagccg atcgatggga gagacgtcga
    12481 tatagccggc atcaactgca aaccgcaggg cggtcttcag aatcgagagc agacggcgtg
    12541 cggcgaccgg gcccttgtcc tcggcatagc gccgcgccac ccggcgcacc tcgaggcgct
    12601 ccagatcacg cagcttcgtt gacttcagtg aggaatcgag ccggtcgatg tagccgcgca
    12661 cgtgatgcgg gcgtcgatag ccttcggtga tgacctcggt gtaccaatcc tccaggagct
    12721 cgccgagcgt gaccttggtg acgcgtttgc cggtgaactc ggctgccttg acgcgggccg
    12781 ccgcgcagcc caagtccggg aacgcgccga gcgtgatgtt gccgccgcga tccttcttgc
    12841 ggcgcaggac ccaggacttg ccccccgtgg tgcggtggcg caggtacagt cccttgccgt
    12901 cacgatgcca cctgtccttc tcaggatcct tgagtgctct gattgcttta tcagttagca
    12961 tctgccgctg tgggatacac ctgggataca cctaggcgcg ggcatgctac tggacaggac
    13021 aggacactac aagacacaat aggaatgcga atcagagagt tgcgattgca agtggctgta
    13081 tgaaaatcct ttttgtttgc ctgggaaaca tctgtcgctc tcccacggcc gcgatcgtct
    13141 tgcgagagat tgccgcgcgc gaggcgcctg cgctgcccat cgaggtggac tccgctggga
    13201 ccgctgacta tcacgtcggg cagccgccgg atcgccgaac ccgggaagct gctcttcggc
    13261 gcggatacga tatgtcttcc ctgcgcgccc gggtcgtcga gtcacaggat tttggccggt
    13321 tcgacctgat actggccatg gatcgagcaa acctcgaggt gctgcgacgg cgagcgccgc
    13381 agagcgccca ggagcggatc cgcctgtttc tggagttcgc gcccgaggca gaggcggcag
    13441 aaatccctga tccttattac ggcggcccga acgggttcga gcacgtgctc gatctcgtcg
    13501 agctcgcagc gcgcgggctg ctactgcacc tgcgctcggc agcgtagcag cccgcgagtt
    13561 cgacttgtcg acccgacctc attcctccgg tccgcgatca cgcggtgtcg gggttgccgg
    13621 gcctgacgac cacacgacca ctggcttggt gggccgctcg gcaccgcttt cggatcccga
    13681 ggttgagatc ggcggtgtcg gctcaaagtg agcgatcggc ctcggctccg gtgatgggga
    13741 gcgttgttcg cccgcctgct cctccgcagc gctgcgctgc gagggctcgt gccgctcctc
    13801 ctgcgagaac tccggaggat gagcaccagt gtccgtgtag ccctgcgtac cattgccctg
    13861 gaatgcggag cgttcatcgg ctgaagccgt tgatgcactc tgcgatccgc tttcgcgggc
    13921 tgcgctgtcg cgtcccccgc cgcctcgccg acctcgccga cgccctcgac ggctacgacg
    13981 ctctcctcgg tcgggtcctc cgttctcacg cagctcgctg cccggaggcg gggcaccttc
    14041 gcctgagcgg ccagctctct cggattcctc ggcgtggctg ctctgctcca ttcgggcgcc
    14101 gttctgctct gtgcgagcca atgagccctc gcgctcggga cgtctctcac ccgatcgctc
    14161 gcgcgggcgg cgatcctgat cccgatttcg gtcgcggctc tcatgccgat cgcgggtctc
    14221 gccgcgatcg tgaccctccg ctcgatcccg cccatcgccg cgctcgcggc gcatcccgga
    14281 atctgcatgc cgggaatgtc gcgagtgatc gcgccgtccg tcgcggcggc gatgtccgta
    14341 atcacggctg ccgcctgatc cgctggacgt tgagcgcgtg ggagttggcg ctgcggccga
    14401 ctctgccgct gccggagccg cttgcggggc gccgccgaag atacctttca gccagcccaa
    14461 gaaaccaccg ccgctgtgtg cgggcgctgg tgttgcaggc gctgcgggcg ccgcgacagc
    14521 cgaaagagga gccggtgttg agggcagcag cgtcgccact gccggagact cggtgggggg
    14581 cttacgctcc ttggcactgc caggctcagc ctccgcgggg gcagtcggca tcagatagct
    14641 caattgccgg ttctcgggca actcgagctc atcatcccgc acgcgccgga tcgagtactc
    14701 cggggtctgg atgttctcgt tgggaacgat gagcagctcg acttcgcttt tgtcctcgag
    14761 gtgccgcagg aattcacgct tctcgttgaa caggtaagtg gcgacaccca cggggacctg
    14821 tgcgatgaca cgcgcggtcc ggtccttgcg cagctcttcg ccgatcagcc ggagtatggc
    14881 cagagccatc gactcgatgc tgcggatgct gccgataccc acgcagcgcg gacacacgat
    14941 gtggctcgat tcgccgagtg aaggccgcaa gcgctgccgc gacatctcca gcaggccgaa
    15001 gcgcgacagc cgcccgatct ggatgcgggc acgatccatc ttgaccgcat cgcgcagccg
    15061 gtcctcgacc tcgcgctgat tcttggtgga ctccatgtcg atgaagtcga tgacgatcaa
    15121 tccgccaatg tcgcggatgc gcaactggcg tgcgatctcg tccgcagctt ccagattggt
    15181 attgagcgcg gtggtctcga tgtcgctgcc gcgggtcgcc cgcgcggagt tgatgtcgat
    15241 cgagaccagc gcctcggtgt agtcgatgac gatgctgccg ccggagggga gctgcacctt
    15301 gtgcgcgtaa gcggactcga tctggctctc gatctgaaag cgagtgaaga gcgcgatgtc
    15361 atcctgatac atcttcaggc ggcgctgggc gtcggccggc atgaagcgct gcatgtactc
    15421 ctgggccttc tggaatgccc cctggtcatc gacgagtact tcaccgatgt cgtccgagag
    15481 gtaatcgcgc atcgcgcgcg tgacggcatc actttcccgg aagacgagga acggggcagg
    15541 gcgatcgttg gcggccgccg cgatctggtc ccactgggtc ttgaggttgt ccaggtccca
    15601 ctgcagctcc tcggcgctgc gtccgacccc tgctgtgcga acgatcgcac ccatgccatc
    15661 ggggatctgc agctggccca tcacttcgcg catctggtcg cggtcctcgc cctcgatgcg
    15721 gcgcgagacc ccgccggcgc gcggattgtt cggcatcagg acgaggaagc ggccggccag
    15781 gctgatgaag gtggtgaggg ccgcgccttt gctgccccgc tcctccttct ccacctggac
    15841 aatgaggtcc tgtccctcgg agagcagctc gcggatgttc atccggccgc cctggggttg
    15901 ctggcggaag tactcgcgcg agacttcctt caatggcagg aagccgtgcc gctgggcccc
    15961 gtaatccacg aagcaggctt cgagggaggg ctctatgcgg gagatgcgtc ctttgtagac
    16021 atttgccttc ttttgttcgc gggagggaag ttcgatgctc agatcgaaca gtttctgtcc
    16081 atcaaccaga gcgacgcgca actcttcttc ttgagttgcg ttgacgagca ttcgtttcat
    16141 gtgcccctac tgtgcagtga ggggccgacg agacggcggg gcgcgacgca cgcgtcacgc
    16201 gcgcgggcac gcccgcgcag cagcgaagat ttcggaaaat gaacaagggc gcgagcgtct
    16261 ggttattcat caatgaccgc ctcaacgagg cggtgcgccg gaaaccttcg gtgaactcta
    16321 ccgacggcgc ggcctgcgga gtctttgtca gacgttcgac gcaagcagcg cgatcttgtc
    16381 ggcccaatac gatggcctcg gtgtggagga ccaactaaca tgcggtgccg gcatctccgt
    16441 caggaggcac gccgggaccg aaccgtggcc gtccagttgc ggacggcgtt cgcgagtata
    16501 ctcccaaagc tttcgtagaa acaatgtctt acgcagaggt cgatgtgtcc gcgcaacaga
    16561 gtggcgaggt gcgccagggt gtccggcact tcgaggtaac cgagaacgag gccggccaga
    16621 ggctcgacaa tttcctgcac aagctgctgg gcgacctgcc gcgcagtcgt ctctatcgcg
    16681 tgatccgcaa aggcgaagtg cgcgtgaatg gccatcgtgt cggtccggac acgaggttgc
    16741 aggcgcgcga caagatcagg attccgccgg ttcggatctc ggcgactccc gagccgggga
    16801 ggccgccagt tgctctgctg gagacgctgc gccgcgcagt cgtgcacgaa gaggccgacc
    16861 tcctcgtgat cgacaagcca tcaggtgtcg cggcccacgg tggcagcggg atcagcttcg
    16921 gagccatcga ggcgctgcgc tggctgcgtc ccgatgagac gctcgagttg gtacatcgac
    16981 tggatcgcga caccagcggc tgtcttctcg tggcgcgcaa ccgcggtgca ctgcgaacgc
    17041 tgcatgcctt gatccggcac ggtgagctgg agaagcggta cctggccttg ttgcagggga
    17101 agtgggagct cggacgtaag cggatagacg tgcccttgcg cactgatgct cgggtgcggg
    17161 gtgagcggac tgtgagggtg gatccgcatg gcaaggcctc ggtgagcgaa ttccggcccg
    17221 tgcagttctt cggcaagcgc gcgactttgg tcgaggtgac cctgctcacc ggccgcaccc
    17281 atcagatccg agttcacgcg cgccacgccg gcaaccccgt cgctggcgat gagaaatatg
    17341 gcgatgccga attcaactcg gccctgcgtg ccttcggact gagccggatg tttctgcacg
    17401 cccacagcgt gagctttcag tggccgggag gcagcgagtt cagcgtcaac acgccgctgg
    17461 cccccgagct tgccagcgtc atcgacgcgt tggggactcg gccgcggcgc taaggcagat
    17521 cgaagccgag tttcctcagg gcctccgcaa gccagatcaa aggcaatccg atcaacgcgg
    17581 tagggtcctt agtttcgaca gtttcgaaga gcgtgatacc cagggcctcc gccttgaatc
    17641 cgccggcaca gtcaaagggc tgctcgaggc ggatgtagcg atcgatctcg gcagccctca
    17701 gcgggcggaa cctgaccctg gtcgtgtcga ggtgcaccag gcgctgaccc tcgggcccga
    17761 gcacggcgca gccggtatgg aaaagtgccg tggcgccgct gagctgcctg agctgcgcgc
    17821 ggcagcgttc ggcatctccc ggtttgtcca gcacggtgct gcccgcggca gcaacctggt
    17881 cgctgccgat cacgactgcg ccgccatggc gggctgcaac gacctgcgcc ttcgcgagcg
    17941 acaggcggac ggcacggtcg ggcggaagct ctccctcgag ttccgcctcg ctgctggagg
    18001 gaggcaccgt gtcgaatgac agtcgcagcc gctggagcag gctgcggcgg taaggggagc
    18061 tggaggcgag aatcagcgct ggcataacta cggcccctgt ccctcgcgcg cctgaaatcg
    18121 ccttcgagaa acaagagctt agacttcgaa cgattttgac ttgggggaac ccgataccta
    18181 ttatacgcgc cccatgtcac cgccctggtc caagccgctg gatatcgacc ggctggcgga
    18241 cagcgaggcg gatgttgcct ttgtcgtctc gctggccgag ctgcctcggc tgcgctcgcg
    18301 gctggaaggc gtttcgggca ccgttgccgg gcatgtccgg ttctcgcgag aggccgggct
    18361 cgtggtggcg gacgtggagg tcagtggaga tgcgacactg atctgccagc gatgtctgca
    18421 gccgatgagt gagcccgtga acagctcggt acgcgtggca ctcattgcaa ccgaggccgc
    18481 cctggctcgc gtgcctgatc agctggagcc ggtactggcg gccggaggcc gcatcagcat
    18541 cggggagttg gtcgaggaag aattgctgct ggcgctgccc atcgtgccgt tgcatgcgcc
    18601 cgcagccgct tgcatcgtcg ctaaggatgc gccgctcgtg agcgaggaca agccggacca
    18661 tacgacgcaa agaccgttcg agcgcctgag tgagctgctc aaacgctgaa gttggaggaa
    18721 gactgaatgc ctgtgcagaa gagccgcaag acaccctcgc ggcggggtat gcatcgatcc
    18781 cacgacgggc tggacaagcc cgctctgtcc accgacccgc aatcgggcga gacgcatctg
    18841 cgtcatcgga tcacgccgga cggcttctat cggggccgca gggtgattga gaaacccgcg
    18901 gatgccgacg acaaggaata agcgcttcgt gcgccagcgt cccctctcgg acgcgcgctc
    18961 gcttgcttga gatcgccatc gatgtgatga gcggggacca tgagccccgc gagtacgtcg
    19021 ccggctctct acacgccctt gccgacgacg cggatctgcg agcgttgctc gtcggccgtc
    19081 ctgaggcgat tcagccgctg ctcgcccacg agtcggcctc cttgcgcgag cgcatcgaga
    19141 tcgttccagc ctcgcaggtc gtggcgatgg gcgactcgcc ccgggaagcg attcgccgta
    19201 agaaagagtc ctccatgcgc ctggcgatcg atctggtcaa ggctgggcgt gcgggggctt
    19261 gcgtcagcgc gggtaacaca ggtgcgctca cggccatggc gcatttcgtg ttgaagaccg
    19321 tggcgggcgt ggagcgcgcg gcgatcattt ccgcgattcc cgcaacgcat ggccacacgc
    19381 aaatgctcga tctcggtgcg aacaccaagg ccacccccga gcagctcctg cagtttgccg
    19441 cgatgggcgc catcatcgcg cgtgacattc acggtctgcg cgatccccgg atcggattgc
    19501 tcaacatcgg cgaggaagac atcaagggac atgagatcgt gcaggccgcg cacgcgctcc
    19561 tcgggcagag cggacttcac tacgtaggat ttgtcgaggg cgacgatatc ttcttcggcg
    19621 atgtggatgt cgtggtgacc gatggtttca ccggcaacgt cgcactcaaa accatggaag
    19681 gtgccgccag actggtcgca aatcgtctgc gcgccgagtt ccacgcctcg tggccggcgc
    19741 gcctcaccgg ctggatggca agaccggtgc tacggcgcgt cgccgcaggc ctggatccga
    19801 atcgctacaa cggggcctgc atggtggggc tcgccggtat cgtggtaaaa agtcacgggc
    19861 gggccgatgg agtcgccttc tcgcgtgcca ttgcgacggc agcgctggcc gcacggcggg
    19921 gtttgaccgc tcacatcgag caggcgctgc gcgacaacaa cagcgtccgt gcacagggca
    19981 catgaggggt tctgtttgat tcactcgcgc atcaccggta ccggctcgca tctgcctgag
    20041 aagatcctca ccaatgccga tctcgagaag ctcatcgaga ccaacgacca gtggatccgc
    20101 gagcgcaccg gaatcgagcg ccgccacatc gccgccgacg gcgagacgac agtcgatctg
    20161 gcagagcgtg ccgccaggcg agctctcgac gccgccggcg tccggcctca ggacgtagac
    20221 ttcatcgcct tcggcacgac cacaccggat ctcgtctttc ccaactgcgg tgtgctgctg
    20281 caggggcgat tgggatgccg cgggggaccc gctttcagtg tcgagaccgc ctgcagtggt
    20341 ttcatgtatg cgctctcgat tgcggataag tacgtgcgct gcggggaatc gaagcgcgcg
    20401 ctggtggtgg gctcggagac gctttcccgg atcacggact ggaccgacag atcgacggcg
    20461 atattgttcg ccgacggcgc aggcgccgtg ttgctcgagc cttcggagca gcccggaatc
    20521 gtttcaaccc atctgcatgc ggatggggcc tacaaggatc tgctctactg cggcggcgga
    20581 gtttccaaag gcttcgatcc cgcgggacgc ctggccatct ccatggtcgg gagcgaagtc
    20641 ttcagggtgg ccatcaccac cctgggcagg gcggtggatg agacgctgga ggccaacaat
    20701 ctcgggcgtt cggccattga ctggctcgtg ccgcaccagg ccaatctgcg catcattcag
    20761 gcgatggcgc gcaagctcga tctgcccatg gaacgggtca tcgtcacgat ccaggatcac
    20821 ggcaacacct cggccgcctc ggtgcccctg gctctggact tcgccatccg cgacggacgc
    20881 atcaagcgcg gtgacctgct gctgctggaa gcgttcggcg gtggcttcac ctggggatcg
    20941 gcgctcgtgc gatattagtt aattggctga aatcaccttt ggtgttttca gcaggccagg
    21001 cctggaaggt tggcccgcac gcgggcgaaa agcgtggttt cgcccgcgaa aaacgggcta
    21061 gttatagcta gggagagagt gcgggctgaa aactcctgcg gtgttttggg tccatgaatt
    21121 ctcccagcag ctcaaccggc atgctattcg cgggcaaaag ccatgtcttt gaccgcgtgc
    21181 ctggatccac tctaggtgtg gcccgccaaa acacctctgg tgttttgggc cagtcaaaaa
    21241 aaacgccgcg ctcggcgcgg cgttttgcca ggaagggcgc tcttacttgg agatcgagcg
    21301 cttgaacatc ctgtcgatct tctcattggt cgcatccacg gccgactggg ccgcctgagc
    21361 cgcagccaac gcctggttcg ccgtgctctg cgcgctgctg gcggactggc tggctgactg
    21421 cgccgtgctg ttagcctgat ccgcggtgga cttggctgcc tgcacgtcgg attgcagctt
    21481 cgctacctgc gacttgaggt ctgcgacatc cgcttggagg ggcttcagat cctgacagcc
    21541 tgcaagccca gccacgactg ccaccgcagc caccttcaaa acgatcgcgc acttcataca
    21601 cgtccccttt gggttgttga ttcgggaggt ccctgaaacg atgcggaggg gggcacgcaa
    21661 gttccgtgcc ctcattcgca agcgcgcaac tgcatcaaat ttaccaaggg aatgcaactg
    21721 cagtcattgc ccttgtctgt cgactgcgac agggatacgc gcgcccgggg cgcatgttaa
    21781 gagaacttcg cgaaaacagc atgcgcagtt ttcttgcagc gaagcaacga tgcgggcaga
    21841 tgctgaatgg agtgtgaacg tggcgtcggg tatccactct ttgttgcgtc agcgcaagct
    21901 ggtttgtcgg cgctgtttgt gcgtgcacgc gtgcgctcgc gaggatgcag gaagttgcgc
    21961 cgtccggatg gagcaggcgt ggctcaggtg ccggttcgcg tcgcggaagc ggcggccggg
    22021 agtgaccgga aagaggagag gtagggtcgg cttgccgccg gccgggatct ggatataatc
    22081 acagtagctg tatgaatgaa caggtggccg aaatgtccga tctgacaccc cggcagacgc
    22141 agattctgcg cctagtccag cgattcatct ccgagaccgg aatgcccccc acccgggctg
    22201 aaatcgcccg cgaactgggc ttcagatcgg ccaatgccgc cgaggagcac ctgcgggccc
    22261 tggcccgcaa gggcgtcatt gccctcgttc ccggcacatc ccgcgggatc cagctcaagg
    22321 acaccatccg cgagcagatg ggcctgccgc tcatcggtcg ggtggccgcc ggcaagccga
    22381 tcttcgccga ggagaacttc gagatgcggc tgcaggtgga cccgggactg ttccagccgc
    22441 ggccgcatta cctgctcaag gtgaccggca tgagcatgaa ggatgcaggc atcctggatg
    22501 gggacctcgt cgcggtccat cggacctcgg aggttcgcag ccgccagatc gtggtggcgc
    22561 gattggaaaa tgaggtcacg gtgaagcgtt atcggcagga aggctcggtc gtgtggctgt
    22621 tgccggagaa cgccgatttc gagcccatca aagtcgatct gaagcaggac ccgctgctga
    22681 tcgaaggcat cgtggtaggg gtgctgcgcc gggggaagac caccggactc atgtaatgac
    22741 ggacgctgcc caacaattcg agcggcgctg cggagtcgca tgagaaatcg ctcatgccgg
    22801 cagcggatga actgtctcgg ttgctcgagc acccggccat ctggcgcggt gtcagtgccg
    22861 cgcggatgga agttctgccc acaggcttca gcgcgctcga tgagcgtctt cccggaggag
    22921 gctggcccgc aagaggattg atcgagatcc tcgtctcccg tttcggtatc ggcgagctgt
    22981 cgttgctatt gccggtattg gccacattga cccggcgacc gatggcacgc tggtgtgtat
    23041 ggactgcacc tgctttccca gggagcgctc ttccatcgga cgggaacgaa agctgtctgc
    23101 tggagccttt cgcgcccgca ctcgtcgcgc acggcattgc tctggagcgc gtcctcgtgg
    23161 tgagagtgcc cgcatcctcc cccagagcgc tctccagcca gactttgtgg gcgttcgagc
    23221 agtcactcgg ctcgggagcg tgcgatgttg taatggcctg ggcacgccgg ccaagagcgc
    23281 gtgagattcg tcgtctgcag cttgccgccg agcgtggacg agcactcggt gtgttggtgc
    23341 ggcctcaacg cgcagcttcc gaggcttcct acgcaatcct gcgcttgaaa ctcgagcccg
    23401 cgactcaggg tgtgcgggtc acgatcctga agagccgcgg aggaaagcgc ggctcggtcg
    23461 agctggcatt gcacgcgagc ggaaattgaa gatgcgacca cgtgtcatcc ggacagatga
    23521 cggccggggc acatcagcgc cagctctgcg gtccccacga ctgaccgctc tgagtcctga
    23581 ggatagccgg aatacctctc aggactgcgc cgagctctgg gccggaatgt gtgtgacgag
    23641 cagcccctcc aaccccggtg agtccacctc atctccgccc ttggagcaac tggcgacccg
    23701 cgcacagcgg ttcacccctc gggtgagcct ggagcctccc gatgggctgc tgctcgaagt
    23761 gaaaggaagc ctgcatcttt tcaaaggtgc agccgggctg atgcaggcgc ttggcgagga
    23821 atacggcgat atcggaaagg tatcctcatt cgcccttgcc ccgacacccc ttgcggcatt
    23881 gctgatggcc cgctggggta aaccgctcat catcaccgat aaggcgcaac tcatcggtca
    23941 tcttgcgccc ctgccgctga agctgatgcg ctggcgagca gacgtgcccg aacgcctggc
    24001 acggatggga gtccgtacgg ttggggaagc cttgcgcctg ccgcgggctg gatttgcgcg
    24061 ccgtttcggt attgcgccgc tggcggatct cgatcggctc accggcaggg ctgcggatct
    24121 gccgcggccg tttttcgcgc gcgaacgctt tcgtcgacgg cgcgagctca cttacgagct
    24181 ggaaaacacc gagcgcatcc tgattgtgct ggcgccgttg ctcacggctt taggggattt
    24241 cctgcggctg cgtcagtgcg gcattctgaa gctcgagtgt ctgctgcggc accgtcacgc
    24301 ccctgcctcg agctgtgtgc tgcgcttggc tgctcccctg gcggatgtct cgcgattgac
    24361 tgagctgctg ggcgagcggt tgagcgcgct cgaattgccg gagccggtgc gcgcctgcga
    24421 gcttcgctcc gggaccctcg tcccttacct cccggcttcc cgttcgctgt ggcagcccgg
    24481 tgagcagggt ggtggcgaaa ctcaatccaa cgagctcatc gagcatttgc gcgcgcgcct
    24541 cggtgccaca gccgtgtgtg gactgcaggt tcttgccggg catcgtccgg aaactgcatc
    24601 gagtgtcaga gagccgaacg caggattcgc tgcagtggat ggatcgccgc cgccgccctg
    24661 gtctgccttt cgccgtcctt tgtggcttct gcctgctccg cagctcctgc agcagaagga
    24721 aggccggccg cgacgccgcg ggcccttgcg cctggccgct gagatggaac gcatcgagag
    24781 cggctggtgg gacggaactg atatcgagcg cgactattac atcgcccggg acagttatgg
    24841 cgtggagctg tgggtttttc gtgagcgcac cgcgccgcat cgctggttcc tgcacggagt
    24901 gttcgggtga attgcgagtc gtcttgcgca gcgccacccc ggcagatccc gtacgcagag
    24961 ctgcattgcc tcagtaattt cacattcctg cgcggtgcct cccaccccca ggagctcatc
    25021 gagcgcgcca tcgagctggg ctacatggca ctcgccatta cggatgaatg ctcgatggca
    25081 ggagtggtgc gggctcacgt ggctgcgcgc gatactgctt tgaagctgct cattggagcg
    25141 gaattccggc tcgaatgcgg cctgaaactc gtggcgatcg cgatggaccg ggccggctac
    25201 ggtcgcctgt gccggctgat cacccgcggt cgatgcgcgg ccgtcaaggg aaaatactgc
    25261 ctcaagcgcg aggatgtgga ggcttcaggt ctgcagcagt gcttcgttct gtggactccc
    25321 tgcgcgcgtc gcgcgacgga tcctcggacg ctggacgaat cgcgatggca ggatgaggcg
    25381 cgctggctga gccagcagtt tggcgatcgt gcccggatcg cagtcgagct gttccgtgag
    25441 ggaacggacc ggcagcacct cctcggactg caatcgctcg gtcgtgagtg gggcttgcgg
    25501 ctcgttgcca gtggtgatgt gcacatgcat gggcgcgagc gccgcaggct gcaggatgcg
    25561 ctgaccgctg tccgcctcaa cgtccccatt gcacaggccg gctggcatct gtacccgaac
    25621 ggtgagcgtc atctgcgtga ctacgagcgg ctggtgcgga tctatcccca agagctgctc
    25681 gcggagagca tcaccatcgc gcaagcctgt acgttctcgc tcgatgagct acggtacgaa
    25741 tatcctcatg agctcgtgcc agctggagaa actccgagca gccatttgcg caagctggtc
    25801 gagaagggcg tccgcaagcg ctggccggaa ggcgtgccgg ggaatgttcg caccacactc
    25861 gaaagtgagc tggctctcat cgaggagctg cgctacgagc cgtacttcct gacagtgcag
    25921 gatatcgtcg cgtatgcgcg cgccaacggc attctgtgcc agggccgggg ctctgccgcg
    25981 aattcactcg tctgctatag cctggaagtc tcggccgtcg ggcctgagcg cggcgctgca
    26041 ctgcttttcg agcgcttcat ctcgcgcgag cgcaacgagc cgcctgacat cgatatcgac
    26101 ttcgaacacg atcggcgcga ggaagtgatc cagtacatct accggaaata cgggcgcgat
    26161 cgagcggcaa ttgccgcaac ggtgatcatg taccggccgc gaagcgcact gcgcgacatg
    26221 gcccgcatct tcggactcga ctccgccagc agcgcacggc tctcgcaggt catgcaatgg
    26281 tgggatggca acgagacgct gcccgcgcgc gtacgcgagg cgggcttcga tatgcaggat
    26341 ccgatcctcc aacgcatcgt gagcctggca agacagctcg tggcgtttcc gggcttcccg
    26401 cgtcatctgt cgcagcatgt cgggggcttc gtgatctccg aaggtctgct ggaggagctg
    26461 gtaccgatcg agaacgcgac gatgccggag cggacggtcg tgcagtggga caaggacgat
    26521 ctgaatgatc tcgggctgct gaaagtggac gttctggcac tcggaatgct gagtgcgttg
    26581 cggcgggctt tcgctctggt gaatgactac cgaggcacgc gccacgtgct cggcggactg
    26641 cccgatcggg atgcggaggt ctacgcgatg atgtcgcgcg cagatacggt cggagtcttc
    26701 cagatcgaat cgcgcgcgca gatgtcgatg ctgccgcgtc tgaaacccaa ctgttattac
    26761 gatctggtga tcgaggtggc catcgtgagg cccggcccca tccaggggga catggttcat
    26821 ccttacctgc agtgccgcgc gaagaacgag ccgatcgagt atccgagcga tgccgtggcg
    26881 gaggtgctgc ggcgtactct gggagtgcct atcttccagg agcaggtgat gcagctggcg
    26941 atcgtggccg cgggcttctc gccgggcgag gcggatcggt tgcgccgtgc gatgggagca
    27001 tggaagaaaa gcggcggcat cgagcatttc cgggacaagc tgctcggtgg catgcaggcg
    27061 cgcggctatg aggaagagtt cgcgcagcgg ctgtaccggc agatgctggg atttggcgaa
    27121 ttcggttttc cggaaagtca cgctgccagc ttcgcgctgc tcgtatacga ctcggcgtgg
    27181 ctgaagctgc acgagccggc ggctttcacc tgtgcgctgc tcaacagcca gcccatgggc
    27241 ttctacgccc cggcgcagct cgtgcgcgat gcgcgggggc atggcgtgga ggtgtggccc
    27301 gtggatgtgc aggaaagcgc ttacgattgc acgctcgcgc gccgggacga tggcgagccc
    27361 gctctgaggc tcgggatgcg catggtgaaa tcactatcgc aggcgggcgc gatgagagtc
    27421 gaagcggccc gtcctgcagg gcccttcacc agcgtgcagg atctggcgag gcgcgccgca
    27481 ctcgaccggg gcgatctcga agcgctggcc gcagcgggcg ccctggcagc cctcagcggc
    27541 aatcgtcatc tggcgtattg ggaagtggcc ggtgccgaac ggcccctgcc gctggctgca
    27601 caggcctctt ccggccaccc gggctcggag ccgctcggca atctgcagga aggccggcca
    27661 ttgctcgccg cgcccaccga agggcagggc atcgttgcgg attacgcctc ggtgggtctc
    27721 acactggagc gccatccgct cgccttgctt cgcgaacggt tgtcacacga tcgtattctc
    27781 aggatggacg agctcgatcg catcgcgcac ggccgcatcg tgcgtacggc cggcatcgtg
    27841 ctgatgcgtc agcggcccgg aagcgcgaat ggagtcactt ttgtgacaat cgaggatgag
    27901 agcggtcagg tcaacgtaat cgtgtgggag aacgtggggc ggcagcagcg ccgtgcgatg
    27961 ctcgaatcgc gcctgctgga ggtccacggg gaattacaat atcaggaggg cgtgagacat
    28021 ctcatcgcgc gccgcctggt tgatcgctcg gccctgctcg gagagttgct gacgcgctcg
    28081 cgcgactttc attgagctcc gctcgcaggc cgcttgcgcg ccctggaccg gcaacttacg
    28141 cgagtgtgaa cagctcctag gtcggcttgc gccggacgcg ggccgaagcg ggctccgtgg
    28201 actcctcctc ggagtcgtcc acgccaatgt cgtagtcatc gaagtcgctg gtcagctcag
    28261 cggtgcgctt gtcctcgagc agccgctcga gacggcgcca cgcgggatcg ccggccttct
    28321 gtccgcgacg cttcgccaga tcgaggtcct tgatgacctg atcgagatcc acgtcggcgt
    28381 cgccgaccac cggctcatcc gcctcctcgt cttcgaattc ctctgattcg gatttctcgc
    28441 tcatgcgccg ataccaggta cggcaggaaa tcgagggcgc gaacttaaat cagtttgccg
    28501 atcgttcgca acggcccggg cctcacttga tgagctgatt catctcgaag atcggcaaca
    28561 gcatcgcaaa tacgatgccc atcacaaaaa gacccatgaa cacgatgagc atggggccca
    28621 gcagacccac cataccggcg agcagactgt ccagctcccg ttcctgactg atggccgcgc
    28681 gctccagcat accctcgagc tccccgctcg attccccgct cgagatcaga tgaatcgtca
    28741 tcggcggaaa catgcggctc atgcccagcg agcgcccgat cggggcgccc tcgcgcaccc
    28801 gcgccgcggc ctctgacacc gcatcgcgca tcggaaggct cgtcacgacc tcccctgaaa
    28861 tgcgcaaagc ctccaaaaca ggcaccgagc tagccgacag gatgctgaac gtccgcgtga
    28921 atcgcgccgt gttgaagccc cgcgtgagct tgccgaccat cggcagccgc agcagcacgc
    28981 ggtggtagcg gcggcggttg cctggattgc gcagccagcg gctgaagagg aaaaccgcga
    29041 tgaccgcccc caccaccatc cagatgcccc agtcgcgcac gaagccgctg gtcgcaatga
    29101 ggatcttcgt gatcagcggc agcttgcctt tcgcctgctc gaagactgcg accaccttcg
    29161 gcaccacgta caccatgagc cccgagacga tggcgaagca catgactgag agcacgatgg
    29221 ggtagagcat cgcagcgagc accttctggc gaagcagctc gcgcccttcg gtgtagtccg
    29281 ccagacgctc caggattcca tcgagcttgc cggactgctc gccggcggcc acggtggagc
    29341 gatagatctc gggaaacacg cgtgggaagt ccgcaagccc atcggcgagc gtgtggcctt
    29401 ccatcacctt ggagcgcaca ccgaggacga tgctctgaac gcgcggcttc tcggtctgct
    29461 gcgagaccgc cagcagcgct tcctccagcg gcaggccggc gcgcacgagc gtggccagct
    29521 ggcgggtgaa cagggacaga tcgccggtgg agacgccccg cgtgagcgtg aaggagcgct
    29581 ggcgccgggc ttccttctgc gcgacctcgc tgacggtgac cggcagcagg tgcttctcgc
    29641 gcagcagctg gcggatgtgc cgcggcgtat ccccttcgag aatccccttg cgttccttgc
    29701 cgcctgtgtc gagggccgtg tattcgaagg cgcccatccg ggctcaatcc tcgcgcgtga
    29761 ctcgcaaaac ttcctctatt gtagtttctc cacgcagaac ttttgcacgg ccatcctcgc
    29821 ggatcgaggg cgtcgtatgg cgggcgtatc gctcgagctc ctgctcgctc gcgccatcgt
    29881 ggatcatggt gcgcatctga tcgtccacca cgatcagctc gtagatgccg gagcggccgc
    29941 gatagccgcc tgcgacgtct ctgccgggcc gatagagcac gggggacggg tcctccggac
    30001 gcaggttgag cagccgccgc tcgtactcgc cggcctggaa ggcctgcttc gtatccggac
    30061 tgagcacgcg taccaggcgc tgggcgagca cgccgaccag gctcgaggag agcaggaagg
    30121 gctcgattcc catgtcgcgc aagcgggtga gcgcgccggc cgccgtgttg gtatgcagcg
    30181 tcgagagcac gagatggccg gtgagactcg cctgcacggc gatctgcgcg gtctcgaggt
    30241 cacggatctc gccgaccatc acgacgtcgg gatcctggcg caggatggcg cgcagccccc
    30301 gcgcaaaggt catctcgacc ttggtgttta cctgcgtctg gccgatgccg tcgatgtaat
    30361 actcgatcgg gtcctcgacc gtcatgatgt tgcgggtatt gtcgttgagc cgctcgagcg
    30421 ctgcgtagag cgtggtggtc ttgcccgagc ccgtaggccc cgtgaccagc aggatgccgt
    30481 gcggcttgtg gatcaagtca tcgatgagag cggcgatctc cggcaccatt ccgagcgcat
    30541 tcaggtccag gcgccccgcc tgcttgtcga ggatacgcag caccacgcgc tcgccgtgac
    30601 cggacggaat ggtggagacg cggacgtcca ccgaacggcc ggcaatcttc aggctgatac
    30661 gaccatcctg cggcaggcgc ttctcggcga tatcgagttt cgacatgacc ttcaggcgcg
    30721 agacgaccag cggtgcgacg gcccgcttgg actgcagcac ctcgcgcaga atgccatcga
    30781 cgcggaagcg gatgaccaga cggttctcga aaggctcgac atggatgtcg gaggcgtttt
    30841 cgcgcaccgc ctgcgtcagc acggcgttga tgagccggat gatgggcgca tcgtcgtcac
    30901 tgtcgagcag gtcagcctgc tcgggcagat cctgcgcgag gtgggcgagg tcggtggtgt
    30961 catccagacc ctcgaccgcc tgcatcgtgt cggagccggc ctcatacgcc tggcgcagca
    31021 gaatatcgaa ctcgccttcc gggactcgct caatgcgcat tggcaggcgc agatagcgac
    31081 gcacctctgc gatcgccaag ggtgagacgt tctcgcggca ggcgcactcg gccccgccct
    31141 cgaccaggcg cttgacgagc accccgtggc gcttggcgaa ggcaaaggaa agccggcgct
    31201 catgcgtcat cggtgccgcg acgtcgttca ctgcttgggc tccggctgcg gggtcgcttc
    31261 cggtgaggtg tcgggtgccg tcctgggcgg cggcggcgtg acgcccagat caggcgaggc
    31321 gctcgcggcg gacccatcgc tctgaggcgg cttgggaggg gcgatcggct gggtgatcgc
    31381 gccgggcttg gacagcttct tagctgcttc ggcatcggct gcggcctgcg ccttggccgc
    31441 cttctccttc tcttccggcg aggcgggcgc gaccggggtc gagcccggcg gcggcggggg
    31501 cggcggcgac ttgagcagcg gcttgttcac acccggcagc agcggcagcc actcacgctt
    31561 gccgttgtag gtcttctcct gctcgatcat gtagttgtat ttcgaatccg cctcgtacgc
    31621 cgcctgcgtc tggtcgcgca ggatcttcgg gcgcatgaag atcatcaggt tgttcttcgt
    31681 ggcctccgtg tgccgggtct tgaagaactg gccgatgatc ggaatgctgc cgaggaaggg
    31741 cacctgctgg ctgtctttcg agttcgagtc cgaaatgagg ccgcccagca ccacgatgcc
    31801 accatcctcg atgagcacgt tggtggtgat ggtacgcttg ctggtcgtga tatccactga
    31861 ggagaccgac gtcggcagca cgctcgagct ctcgatggag atcttgagca ccaccgagtt
    31921 gccttccgcg gcaatgttgg gggtcacctt gaggatcgtg cccacttcct cgcgctgaat
    31981 cgtctggaag ggattgaccg cgccgttgct gaccgtgccg gtgttggcga acgagccggt
    32041 gacgaaggg
//
LOCUS       DASRHN010000187        11806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1384788, whole genome shotgun sequence.
ACCESSION   DASRHN010000187 DASRHN010000000
VERSION     DASRHN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11806
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384788"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFB37_07325"
     CDS             <1..516
                     /locus_tag="VFB37_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZO22303.1"
                     /translation="IREDDSLRERLAYLQNAVGGVSGPFDSFLTLRGIKTLALRMQQH
                     CSNAAAITDFLQGHPRIERVYYPGLPSHPQHELAQRQMTGGFGGIVTAVLRASLDETR
                     RVLERCTLFSLAESLGGVESLIEHPGLMTHASLPAQMRASLGISDGLIRLSVGIEHPA
                     DLIEDLRQALG"
     gene            complement(553..1650)
                     /locus_tag="VFB37_07330"
     CDS             complement(553..1650)
                     /locus_tag="VFB37_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZO22304.1"
                     /translation="MLLKPTVFYFCRLGDMVMLTPALKLLHTRYGRPCQVIGTGTWTG
                     EMYEGHPDVSDVWSFHRHLPFVLDTPWRSLRRALRESHPGPIYVCEQHYRQLPRIRRM
                     LQLSGIDPRRCVYPNDQPLVAPEHLVDRLVRLALRTPDLLRASDYLPPSLRSIDGPRL
                     HVLAHERRERDDWLKSLDWYGRELILLQPGNHRTMGWRRDRWRRRNTDDKWWPLERWV
                     KLLGHVHEVREEAVLVLCGSITEVPMLEQLRAAAQLPRLGLFSGTLRQLFALCEAGHS
                     MISVDTGPAHVAGALGLPIVVLFGAQPAEYWLPRTRGSAAVGVGGPPRSTRVDQISVD
                     EVVTAWHTVIDRRASGQCVHADALEFGRSPA"
     gene            complement(1678..2811)
                     /locus_tag="VFB37_07335"
     CDS             complement(1678..2811)
                     /locus_tag="VFB37_07335"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HZO22305.1"
                     /translation="MPRQIRMPLLVPFILALTPSSMLAAKAVGASSAVPQLEPAAAAR
                     FADLALKCLHREYPNKIAHVLHSDADALPPHALTPAFYGCFDWHSDVHGHWLLVRLLR
                     LFPQARFAAEARAALGASLTPGHIAGELAYIRGADRASFERPYGLSWLLKLAAELRNW
                     NDPQAQQWSATLVPLESEAAARLESWIPKLHYPIRIGEHDQTAFSFGLMWDWAGGAHD
                     ARMRELLKDAAQRFYKQDRDCPLSYEPSGEDFLSPCLAEADFMRRVLDQAAFAAWLRT
                     FLPQIPPRGGADWLLPGIVTDRADPKLAHIDGLNLSRAWMLEGIAAALSRDDSRRPAL
                     LAAAARHRAESLPAVTGEHYEGGHWLGTFAVYLTSEAGLAPGR"
     gene            2994..3881
                     /locus_tag="VFB37_07340"
     CDS             2994..3881
                     /locus_tag="VFB37_07340"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO22306.1"
                     /translation="MNIGFIGLGKMGQAIAARLVHAGHRVRVWNRSAGPVKELVERGA
                     EAAARPAETARGELLVSMLADDAAMRAVLLDKGVLEAAAPGLIHLNLATISVALAREL
                     TELHRRYEVSYVAAPVFGRPEVAAEGKLNIVVAGEPAAIERAKPALSAIGQKIWPVGE
                     RPEAASAVKIAGNFMIASAIEAMGEAAALARAHGIGAKEFMEIVTGSLFAAPVYKGYG
                     ALIAEERYQPAGFAVTLGLKDIRLALAAGDAAHVPMPFAALLRDNLLELIATGSAGLD
                     WACLAGLAAQRAGLEHRGS"
     gene            3955..5208
                     /locus_tag="VFB37_07345"
     CDS             3955..5208
                     /locus_tag="VFB37_07345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO22307.1"
                     /translation="MTPAQPGGAVAVAPVSFAALRHPGFRMYFLGTATAMLADNIEHV
                     ISYWAIYQKFHSPLLGGVAVISHWAPFLLFSIYSGALADRFDPRRIIQIGMLLFMGVS
                     IAWGVLFLTGWLRESHAWLLLIVHGFAGVLWMPASQLLLHDIVGPEELSSAVRLGATA
                     RYLGMLTGPGVGAVLLLALGPARGILVNSLIYLPMLLWLWKAPYGPRFRRRDAVRTRE
                     VRGFADLVAAVRSIRNNSTVLCMILLAALAALFIGNAYQAQMPGFAVDLGHGDPGLSY
                     SMLLAADAAGALTAGFVLESRGLLHPAARTALILAMLWCISLGVFALVRLYPLALALL
                     FAAGFLELSFNSMAQALVQLNAPAAVRGRVIGAYSMAASGMRMFSGLTVGIAGGLIGI
                     HRSLALSAAALFIAVATLFSFTSRR"
     gene            complement(5213..6601)
                     /locus_tag="VFB37_07350"
     CDS             complement(5213..6601)
                     /locus_tag="VFB37_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO22308.1"
                     /translation="MKLSRHLLIGAATLLASMSGAHAASIVIEHVTLIDGTHAPQPDM
                     SVAIEGPRISRVAPSASARGLVGRRIDGRGKYLIPGLMDVHIHLEGGFEGGGSSSGGM
                     PPPNREKGVAALASFLYAGVTTVYDAGNRAEHILPLRADERAGRILSPRIFATGNLIT
                     YPGSHGDRIAVRISDFEKDKALLDRHIAEQQPDMLKLTLEEEGWGSRPMIPLMPVELL
                     GKIIRYYNQHGIRTTVHVSSELRALEAIYAGSDTLAHPVIQGPVSESFVKLMAAKKIP
                     FASTLTIGDNYNRLVDHPEYLDQPLYVASLSAQERESLKTQTRAEWKTRPWTAWMKVM
                     TPIAEENVRKIDAAGGIVACGTDQSSGAATQRELQLLAASGIAPLEVIRIATYNSAVF
                     LGRADQLGSVESGKLADLVLLSRDPSLNIENASSIVLVMKNGQIIDESQLPLAGGRQP
                     RRYSGPGVTASM"
     gene            complement(6657..7229)
                     /locus_tag="VFB37_07355"
     CDS             complement(6657..7229)
                     /locus_tag="VFB37_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZO22309.1"
                     /translation="MYRAPEGVPRRGRYRLGLALGVLAVSVARAAAPEPAGFWTGPVN
                     SPVPATVRGGKVIHARKLSELLKGRRAVIVDVSNAPRRPENLPASTPWLPLPHQAIPG
                     AIWIPGAGLGAIPGPIEKFYRERLASATGSDTSRTVVVYCHARCWLSWNGAKRAIQYG
                     YRNVYWFPDGIEGWRAAHLPTRVIAPEPTP"
     gene            complement(7213..8025)
                     /locus_tag="VFB37_07360"
     CDS             complement(7213..8025)
                     /locus_tag="VFB37_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HZO22310.1"
                     /translation="MGVMRARAVAAALACLALVPFGPAAHADDDDADRGARWKQLQES
                     IFPHRQVQDGGGVISIDAPPRALDAALVPVQVQIAGGKAVKAVYLIIDDNPAPLAAHF
                     VFGPKADPHDLKLRVRVNQYTNIHAIAESTDGQLYATAKFVKASGGCSAPAGSDDAAA
                     LQDIGRMKLRLLSPFNAGKPLQAQLMIRHPNFNGMQMNQLTRFYTPARFIRTIDATYN
                     GGQVFHMDADISLSTDPVITFGFVPREKGQMKVVAQDSSNSTFDHSFDVPGT"
     gene            complement(8016..8978)
                     /locus_tag="VFB37_07365"
     CDS             complement(8016..8978)
                     /locus_tag="VFB37_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HZO22311.1"
                     /translation="MKQRWSRREVLLGTGAVCLGAGTLSVSELARSAVSPPHAFTTSE
                     VAAGIHVRRGVDEDVTADNDDAIANIGFVIGRDAVAVMDPGGSLADGTRLRAAIRKAT
                     NLPIRYVILSHVHPDHIFGAGAFSADGPQFVGHARLPNALALRGDYYRSRLEQVLGKG
                     RAGPVVVPKLLVHDHLPIDLGGRVLELTAHGLAHSDCDLSALDRQTGTLLASDLLFVQ
                     RVPSLDGSLKGWLHELAELKRVGARRAVPGHGPASVDWPSAAQPLQRYLETLQRETRQ
                     AIATGRDIEQAVSTVALSERDRWKLFDDYNGHNVTRAYKELEWE"
     gene            complement(8975..10300)
                     /locus_tag="VFB37_07370"
     CDS             complement(8975..10300)
                     /locus_tag="VFB37_07370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO22312.1"
                     /translation="MADASWDVVIIGAGVAGLSAARALRECDCSVLILEARDRVGGRV
                     WTRHEPDLAAPIELGAEFIHGRVPETLELLQAAGKAALDTSGAHWSMRQGRLEQRTED
                     LFAQIQSALERAHVLQQPDLSFGAFLDRSVQQGLSPEARNMARTFVEGFDAADPARVS
                     THFVAKEWGSGGMLDDAQLRPADGYSSVLAALAGELGRDRIRLQLQTQVRQVRWKPGA
                     VQIDAIVQDQPFQARAARAIVTLPLGVLQSQDSANFVQFIPSLDKKREALSGLASGAV
                     LKLSLRFRRAFWERLDGGRYRDAAFFHSPGAAFPTFWAPRPLRAPLLTAWVGGPKAAR
                     LSERPPHEIVAEALASLAALFHTAPAGAFELEATYFHNWQRDPLSQGAYSYVLVGGTG
                     ARRLLAAPLEQTLFFAGEATEEDAATVPSALRSGTRAARELRQSMGMRA"
     gene            10348..10920
                     /locus_tag="VFB37_07375"
     CDS             10348..10920
                     /locus_tag="VFB37_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HZO22313.1"
                     /translation="MPAPVAQRIALSPHPSTPADAIGGLEVEVRAEAGAVLALGYRLW
                     ADMARIRLPGSGEAIDTAAGRAEELWKHTCFEAFLADARSSGYCELNFSPAGQWAAYH
                     FDEYRRGMRALELFGPPQIAVRRSPERLELDALVRLPREREAGPRKLALCAVIEEENG
                     RLHYWAAQHPEGKPDFHHPDGYVLELTFRP"
     gene            10917..>11806
                     /locus_tag="VFB37_07380"
     CDS             10917..>11806
                     /locus_tag="VFB37_07380"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HZO22314.1"
                     /translation="MKFGIDRLLDEPELRRPLLGRRVGLLAHPASVTRNLVHSLDALC
                     ALGDTQVTAAFGPQHGLRGDKQDNMVESGDFRDPVHGIPVFSLYGTVRRPTAEMMDTF
                     DVLLVDLQDVGCRVYTFVTTLRYVLEEAAKRRKSLWVLDRPNPVGRPVEGLTLRPGAE
                     SFVGAGPMPMRHGLTMGELALWFVTRLRLDVDCQIIPMDGWEPALEPGYGWPAGRRTW
                     VNPSPNAPGLGMARCYPGSVMLEGTTLSEGRGTTRPLEGLGAPDVDARALLAEMRAVA
                     PAWMRGCRLRSCWFEPTFHK"
BASE COUNT         1927 a         3850 c         3963 g         2066 t
ORIGIN      
        1 atcagagagg atgactcgct gcgggagcga ctggcctatc tgcagaacgc agtgggcggc
       61 gtgtcgggtc cgttcgactc tttcctgact ctgcgcggca tcaagacgct cgcattgcga
      121 atgcagcagc attgctcgaa cgccgcggcc atcacggact tcctccaggg gcatcctcgg
      181 atcgagcgtg tgtactaccc gggcctgccg agtcaccccc agcatgagct ggcgcagcgc
      241 cagatgaccg gcggattcgg cgggatcgtg accgccgtcc tgcgcgcgag tctcgatgag
      301 acacggcggg ttctggagcg gtgcacgctc ttctcactcg ccgaaagtct cggcggcgtg
      361 gagagtctca tcgagcatcc aggtctcatg acacatgctt cgctgccggc gcagatgcgt
      421 gcttcgctgg gaatcagcga tgggctgatc cggctgtcgg tggggatcga gcatccggcg
      481 gacctgatcg aggacctgcg ccaggctctc ggctgatcat tcaattcgcc ggcgctcgcg
      541 cgacccgcaa actcacgcgg gcgaccgccc gaactcgagg gcatcggcgt gaacgcactg
      601 ccctgaggcc ctccggtcga tgacggtgtg ccaggcggtg accacctcat ccacggatat
      661 ttgatcgaca cgcgtcgatc gcggaggccc gccgacaccg acggcggcgg agccccgcgt
      721 gcgcgggagc cagtattcgg ccggctgagc gccgaacagc acgacaatgg gcaggccgag
      781 cgcgcctgcc acgtgagcgg gaccggtgtc caccgagatc atgctgtggc cagcttcgca
      841 cagcgcgaac aactgccgca gagtgccgct gaacagtccc aacctgggta gttgcgcggc
      901 cgcccgcagc tgctccagca tcggcacctc ggtgatcgat ccgcacagca ccagcacggc
      961 ctcctcgcgg acctcgtgca cgtgacccag cagcttcacc cagcgctcga gcggccacca
     1021 cttgtcgtcc gtgttgcgac ggcgccagcg gtcgcgccgc caacccatgg tgcgatgatt
     1081 tccgggttga aggaggatca gctcccgccc gtaccagtcg agagacttca gccagtcgtc
     1141 acgttcgcgg cgctcgtgtg ccagtacgtg cagccgcggc ccgtcgatcg accggagtga
     1201 tggcggcagg tagtcgctgg cgcgcagcag gtccggtgtc cgcagcgcca gccgcaccag
     1261 ccggtccacg agatgctctg gggccaccag cggctgatcg ttcggataca cgcagcggcg
     1321 cggatcgatg ccactgagct gcagcatgcg ccgtatccgc ggaagctgcc gataatgctg
     1381 ctcgcagacg taaatcgggc cgggatgaga ttcccgcaaa gcgcgccgca gggatctcca
     1441 tggcgtgtcg agcacaaacg gcaggtgacg atggaacgac cagacatctg acacatccgg
     1501 atgaccctcg tacatctcgc cggtccacgt tccggtaccg atcacctggc agggccggcc
     1561 ataccgggtg tgcagcagtt tcagcgccgg ggtgagcatc accatgtcac ccaggcgaca
     1621 gaagtagaag acagtcggtt tgaggagcaa tcggatgacc tgcagcccgc caggagttta
     1681 tcggcccggt gccagaccgg cctcgctggt caggtagacc gcgaaggtac ccagccagtg
     1741 ccccccttcg taatgctcgc cggtgacggc gggtagcgac tcggcgcgat gccgggcggc
     1801 cgcggcgagc aacgccggac gtcgcgaatc gtcgcgactc agcgccgccg cgattccctc
     1861 gagcatccag gcgcgactga gattgaggcc gtcgatgtga gccagcttgg gatccgcgcg
     1921 gtccgtgacg atgcccggca ggagccagtc ggccccaccg cgcgggggaa tctgcggcag
     1981 gaaggtgcgc agccacgcag cgaacgcggc ctgatcgagc acgcggcgca tgaaatccgc
     2041 ctcggcgagg cagggggaga gaaaatcctc acccgagggc tcatagctca acggacaatc
     2101 ccggtcctgc ttatagaaac gctgcgcggc atccttgagc agctcgcgca tgcgagcgtc
     2161 gtgcgcgccg ccggcccagt cccacatcag gccgaacgaa aaggcggtct ggtcgtgctc
     2221 tccgatccgg atcggataat gcagcttggg gatccagctc tcgaggcggg cggccgcctc
     2281 gctctcgagc ggcacaagcg tagcggacca ctgctgagcc tgcgggtcgt tccagttgcg
     2341 cagctcggca gcgagcttca gcagccagga caggccgtag ggccgctcga aggaggcgcg
     2401 gtctgctcct cgaatgtagg cgagctcgcc cgcgatatgt ccgggtgtca ggctcgcgcc
     2461 gagcgcggcg cgtgcttcgg ccgcgaaccg agcctgcgga aacaaccgta gcaggcgcac
     2521 gagcagccag tggccgtgca cgtcggaatg ccagtcgaaa cagccataga acgcaggagt
     2581 cagcgcatgc ggcggtaatg catccgcgtc gctgtgcagc acatgcgcga tcttgttggg
     2641 atactcacga tgcaggcact tgagcgcgag atctgcgaag cgcgccgcgg ccgcaggctc
     2701 gagttgcggg accgcgctgc tggcaccaac agccttcgcg gccagcatac tggatggcgt
     2761 aagtgcgagg atgaagggca ccagcagcgg catgcgaatt tgtcttggca cgagtctccc
     2821 catcgccgat cggatctgtg cctggctgtc tcgcaggagg gccggcgagg catgttaacg
     2881 cggtgtggcc cggccaatcg agaactgccc agcccgggca cgccgcgcag cctcggtccg
     2941 gctcctccct gggaccgatc cttgctttga ctgccgccca cggagagcct cacatgaaca
     3001 tcggattcat cggccttgga aagatgggac aggcgattgc cgcacgcctc gtgcatgcgg
     3061 gccaccgcgt gcgggtctgg aatcgttccg cgggaccggt taaggaattg gtagagcgtg
     3121 gtgcggaggc cgcggcacga ccagcggaga ccgcacgcgg cgagctgctc gtgagcatgc
     3181 ttgcggatga tgcggcgatg cgcgcggtgc ttctggacaa gggcgtgctt gaggcggcgg
     3241 cgcccggtct gatccatctc aacctggcca ccatctcggt ggcgctcgcc cgcgagctga
     3301 ccgagctcca tcgccggtac gaggtgagct acgtggcggc acccgtgttc ggccgaccgg
     3361 aggtggccgc tgaagggaag ctcaacatcg tcgttgccgg cgagcccgcc gcgatcgagc
     3421 gagcaaagcc agcactgtct gcgatcggcc agaagatctg gccggtcggg gagcgcccgg
     3481 aagcggccag cgccgtgaag attgccggca acttcatgat tgccagtgcc atcgaggcaa
     3541 tgggcgaggc agcggcgctg gcacgcgcgc acggaatcgg cgcaaaggaa ttcatggaga
     3601 tcgtgaccgg gagcctgttc gcggctccgg tgtacaaggg ctacggcgcc ctgatcgccg
     3661 aggagcgcta tcagccggca ggctttgccg tgactctggg attgaaggat atccgcctgg
     3721 cgctggcagc gggcgatgcg gcgcatgtgc cgatgccctt cgccgcactg ctgcgcgaca
     3781 acctgctgga gctgatcgcc accggctcgg ccggactcga ttgggcctgc cttgccggcc
     3841 tggccgcaca gcgcgcagga ctcgagcacc gaggcagttg aacctggctc cgcagggctt
     3901 ctgaaccgaa ggctggattt cgctacactc gccgctcttc gaccgggagc cggattgacc
     3961 ccagcccaac ccggcggcgc tgtcgccgtc gcgcccgtct cattcgcagc gttgcgccat
     4021 ccgggcttcc ggatgtattt cctcggtacg gcgacggcga tgctcgcgga caacatcgag
     4081 cacgtcatca gctactgggc catttatcag aaattccact cgccgctgct gggcggcgtc
     4141 gcggtgatct cacactgggc gccctttctg ctgttctcga tctattccgg ggcgctcgcc
     4201 gaccgcttcg atccgcggcg gatcatccag atcggtatgt tgctgttcat gggcgtgtcg
     4261 atcgcctggg gtgtgctctt cctgacgggc tggctgcgcg agagtcatgc atggctgctg
     4321 ttgatcgtgc atggatttgc cggcgtcctg tggatgcccg caagccagct cctcctgcat
     4381 gacatcgtgg gtcccgagga gctgtcgagc gcggtacgcc tcggagccac cgcgcgttat
     4441 ctcggcatgc tcacgggtcc gggggtgggc gccgtgttgc tgctcgcgct cgggccggcg
     4501 cgcggcatcc tggtgaactc actcatctac ctgccgatgc tgctctggct ctggaaggca
     4561 ccctatggcc cgcgctttcg ccggcgcgac gccgtgcgca cccgggaggt gcgcggcttt
     4621 gctgatctgg tggccgctgt gcggagcatt cggaataact ccacggttct ctgcatgatt
     4681 cttctggctg cgctggccgc gctgttcatc ggtaatgcct atcaggcgca gatgcccggc
     4741 tttgcggtcg atctcgggca cggcgatccc ggcctttcct acagcatgct gctcgccgcc
     4801 gacgcggccg gtgcgctgac tgcgggattc gtgctggaga gccgtggcct gctgcacccg
     4861 gcggcccgta cggcgctgat cctggcgatg ttgtggtgta tctcgctcgg cgtattcgct
     4921 ctggtgcggc tctacccgct cgctctggcc ctgctgtttg cggctggatt cctcgagctg
     4981 tcgttcaact cgatggcgca ggcgctggtg cagctcaacg ccccggccgc agtgcgcggg
     5041 cgggtgatcg gggcctactc gatggccgcc agcgggatgc gaatgttcag cggattgacg
     5101 gtcggcattg ccggcggtct catcggcatc caccgctcgc ttgcactgag cgccgcggcg
     5161 ctgttcatcg cagtcgcgac cctgttctcc ttcacttcgc ggcgttgatc cttcacatcg
     5221 aggcggtgac acccggtccc gagtagcgcc gcggctgccg gccgcctgcc agcggcaatt
     5281 ggctctcgtc gatgatctgt ccattcttca tgaccaacac gatggagctg gcgttctcga
     5341 tgttgaggct agggtcgcgg ctcaacagca cgagatcggc cagcttgccg ctttccaccg
     5401 agccgagctg atcagccctg cccagaaaca ctgcgctgtt gtaagtcgcg atccggatga
     5461 cctcgagcgg cgcgatgccg gaagctgcca gcagctgcag ctcccgctgc gtcgccgcgc
     5521 cgctgctctg gtcggtgccg caggcgacga tgccgccggc tgcatcgatc tttctgacgt
     5581 tttcctcggc aatgggcgtc atgactttca tccatgccgt ccagggacgt gtcttccact
     5641 ccgcgcgcgt ctgcgtcttc aggctctcgc gctcctgtgc gctcagtgaa gccacgtaca
     5701 gcggctgatc gagatactcg gggtgatcga cgaggcggtt gtagttgtca ccgatcgtga
     5761 gcgtactggc gaaggggatc ttcttcgccg ccatcagctt cacgaatgac tcgctgaccg
     5821 gcccctggat gaccggatgc gccagagtat cgctgccggc atagatcgcc tcgagcgctc
     5881 gcagctcgct cgagacgtgt accgtggtgc ggatgccgtg ctgattgtaa tagcggatga
     5941 tcttcccgag cagctccacg ggcatcaggg gaatcatggg tcgcgatccc cagccttcct
     6001 cctccagtgt cagtttcagc atgtcgggct gctgctcggc aatgtgacga tcgagcagcg
     6061 ccttgtcctt ctcgaagtcg ctgatgcgca cggcgatgcg gtcaccgtgg ctgcccggat
     6121 aagtgatgag gttgccggtg gcgaagatgc gcggcgacag gatgcggccg gcacgctcgt
     6181 ccgctcgcag cggcaggatg tgctcggcgc gattgccggc atcgtagacg gtcgtcacgc
     6241 ccgcatagag gaagcttgcc agtgcggcaa cgcccttttc gcggttgggt ggcggcatcc
     6301 cgccgctgga actgcccccg ccctcgaatc cgccttcgag gtggatgtgc acgtccatca
     6361 gacccggaat gagatatttc ccccggccgt cgatgcgccg gccgaccagc ccgcgcgcgc
     6421 tcgcgctcgg tgcgactcgt gagatccgcg ggccctcgat cgcaacgctc atgtcaggtt
     6481 gaggggcatg cgtgccatcg atgagcgtga catgttcgat gacgatggag gcagcgtgtg
     6541 ccccgctcat ggacgcgagc agcgtggcgg cgccgatcag caaatggcgg ctgagcttca
     6601 cttggatctc tccgggagtg cgatgccagt cgaaacagcc gcggcgaacg agcgcgctag
     6661 ggagtgggct cgggagcgat cacgcgggtc ggcagatggg ctgctcgcca gccttcgatg
     6721 ccatcgggaa accagtagac gttgcggtac ccatactgga tcgcacgttt tgcgccgttc
     6781 cagctcaacc agcagcgcgc gtgacagtac acgacaaccg tccggctggt gtcgctgccc
     6841 gtcgcgctgg ccaggcgttc gcggtaaaac ttctcgatcg ggccgggaat ggcaccgagg
     6901 ccggcgcccg ggatccagat cgcccccgga atggcttgat gcggcaaggg cagccacggc
     6961 gtagaggcag gcagattctc cggtcgccgg ggcgcgttgg agacgtccac gatgacggcg
     7021 cgccgacctt tcaacaattc ggatagcttg cgggcgtgaa tgaccttgcc gcctcgcacg
     7081 gtggcgggaa ccgggctgtt gaccggccca gtccagaaac ccgcaggctc gggggcggcg
     7141 gcgcgagcaa cggagacggc aagaacgccg agcgcgagcc cgagccggta ccggccgcgc
     7201 cgcggaactc cctcaggtgc ccggtacatc gaagctgtga tcgaaggtcg agttgctgct
     7261 gtcctgagcg acgaccttca tctgcccctt ctcgcgcggc acgaacccga aagtgatgac
     7321 cgggtcggtg ctgagcgaga tgtcggcgtc catatggaac acctggccac cgttgtaggt
     7381 cgcatcgatc gtgcggatga agcgtgccgg ggtgtaaaag cgagtgagct ggttcatctg
     7441 catgccgttg aaattcggat ggcggatcat gagttgcgcc tgcaacggct tgccggcatt
     7501 gaatggactc aacagccgca gcttcatccg accgatgtcc tgcagggcgg cggcatcatc
     7561 ggatcccgca ggcgccgagc agccaccgga ggccttcacg aactttgccg tcgcgtacag
     7621 ctggccgtcc gtactctccg cgatcgcatg aatgttcgtg tattgattca ctcgcacgcg
     7681 cagcttcaga tcgtgtggat cggccttcgg tccgaaaacg aaatgcgcag ccagcggcgc
     7741 cggattgtca tcgatgatga ggtagaccgc tttgacggct ttgccgccgg caatctgcac
     7801 ctggacgggg accagggctg catcgagtgc acgaggcggg gcgtcgatcg agatgactcc
     7861 gccaccatcc tgcacctgcc ggtgcggaaa gatcgattcc tgcagctgct tccagcgcgc
     7921 gccgcggtct gcgtcgtcgt cgtcggcgtg ggcagcagga ccgaagggaa ccagcgccag
     7981 acaggcgagg gctgctgcga cggcgcgcgc gcgcatcact cccattcgag ctccttgtaa
     8041 gctctcgtga cgttgtgccc gttgtaatcg tcgaacagtt tccatcggtc tcgctctgac
     8101 agtgccacgg tgctgacggc ctgctcgatg tcgcgtcccg tggcaattgc ctggcgcgtc
     8161 tcgcgctgca gcgtttccag gtagcgctgc aggggctgcg ctgcagatgg ccagtctacg
     8221 ctcgccggac catgaccggg tactgcgcgc cgcgcgccca cccgcttgag ctccgccagc
     8281 tcatgcagcc atcccttgag actgccgtcg agcgagggca cgcgctgcac aaaaagcagg
     8341 tccgaggcga gcagcgtccc ggtctgacga tccagggcgc tgaggtcaca gtccgagtga
     8401 gcgagcccgt gcgccgtcaa ttccagcacg cggcccccca ggtcgatcgg caggtggtcg
     8461 tggaccagca gtttgggaac cacgaccggt ccagcccgac ccttgccgag cacctgctcc
     8521 agccgggaac gatagtagtc cccgcgtaat gcgagcgcgt tgggcaggcg cgcgtgccct
     8581 acgaactgcg gtccgtcggc gctgaaggcg cccgccccga agatgtggtc ggggtgcacg
     8641 tgcgagagaa tcacgtagcg aatgggcaga ttcgtggctt tgcgaatggc cgcgcgcagg
     8701 cgcgtgccgt cggcgagact tcctcccgga tccatgacgg ccacggcgtc ccgaccgatc
     8761 acgaatccga tgttggcaat cgcatcgtcg ttgtcagccg tgacatcttc atcgactcca
     8821 cggcgaacat ggataccggc agcgacctcg ctcgtcgtga acgcgtgagg ggggctgact
     8881 gcgctgcggg cgagctccga taccgacagt gtccccgcgc ccaggcacac tgccccggtg
     8941 cccaacagca cctcgcgccg ggaccagcgc tgcttcacgc tcgcatcccc atgctttgtc
     9001 gcagctcacg cgccgcacgc gtgccactgc gaagcgcgct gggcacggta gccgcatcct
     9061 cctcggtggc ttctccggcg aagaacaagg tttgttcgag gggggccgcc agcaaacgcc
     9121 gcgcgcccgt accgcccacc aacacgtagc tgtaggcacc ctgcgagagt gggtcgcgct
     9181 gccagttgtg gaagtaggtt gcttcgagct cgaacgcccc tgccggcgcc gtgtggaaga
     9241 gcgccgccag gctggcgagc gcctctgcca cgatctcgtg cggagggcgt tcggacaggc
     9301 gcgcggcttt gggtccgccg acccaggcgg tcaacagggg ggcccgcaaa gggcggggcg
     9361 cccaaaatgt cggaaacgcc gcccccggag agtggaagaa cgccgcatcc cggtaacggc
     9421 ccccatccag acgctcccaa aaggccctgc ggaatcgcag gctgagcttc agcaccgctc
     9481 cggaggccaa gcccgacagg gcctcacgtt ttttgtcgag cgatggaatg aactgcacga
     9541 agttcgccga atcctgcgac tgcagcacac ccagcggcag ggtgacgatg gcgcgcgcgg
     9601 ctcgtgcctg gaagggctga tcctgcacga tcgcatcgat ctgcaccgcg cccggcttcc
     9661 atctgacctg tcggacctgg gtctgcagct gcaaccggat ccggtcccgc ccgagctcgc
     9721 ccgccagtgc agccagcacc gagctgtagc catccgcggg ccgaagctgt gcatcatcca
     9781 gcattccgcc cgagccccac tctttggcga cgaaatgtgt gctcacccgc gccgggtcgg
     9841 cggcgtcaaa gccttcgacg aaggtgcgcg ccatgttacg ggcttcgggc gagagaccct
     9901 gttggacgct tcgatccaga aacgccccga aggagaggtc cggctgttgc agcacgtgag
     9961 ctcgctcgag ggcactttgg atctgcgcga aaaggtcttc ggtccgttgc tccaggcgac
    10021 cttgccgcat gctccagtgc gctcccgatg tatcgagtgc cgctttgccg gcagcctgca
    10081 gcagctccag agtctcaggc acgcgtccat ggatgaattc cgccccgagc tcgatcggcg
    10141 cggcgaggtc cggctcgtgg cgtgtccaca cgcgcccgcc gacacgatcg cgtgcctcga
    10201 gaatcaggac cgagcaatcg cactcgcgca gtgcgcgggc tgccgaaagc ccggcaaccc
    10261 ccgcgccgat gatcactaca tcccaggagg cgtccgccat gtctcgcatc ccgcaagtgc
    10321 tgtgccagga attgaataga atgtgccatg cccgcgcccg ttgcacagcg gatcgccctt
    10381 agcccccacc cgtccacgcc agctgatgcg atcggcggat tggaggtcga ggtgcgtgcc
    10441 gaagccggag ccgtcctggc gctgggttat cgcctctggg ctgacatggc gcgaatccgc
    10501 cttcccggct cgggggaggc gattgataca gccgcgggtc gcgcggagga gctgtggaag
    10561 catacgtgct tcgaggcgtt cctcgcggat gcgcgctcga gcggctactg cgagctcaac
    10621 ttctcgcccg ccggtcagtg ggccgcctat cacttcgacg agtaccgccg cggcatgcgt
    10681 gcgctggagc ttttcgggcc gccgcagatt gcagtgcgcc gctcacctga gcggctggag
    10741 ctggacgcgc tcgtgcggct gccgcgcgag cgcgaagcgg gccctcggaa gctggcgctc
    10801 tgtgcggtga tcgaggagga gaatggtagg ctgcactact gggcagccca acaccctgaa
    10861 ggcaagcccg atttccacca tccggacggc tacgtcctcg agctgacctt cagaccatga
    10921 aatttggaat cgatcgactg ctggatgagc ccgagttgcg ccggccgctg ctggggcgcc
    10981 gtgtggggct gctcgcgcat ccggcttccg tgacccgcaa tctcgtccat tctctcgatg
    11041 cgctgtgtgc actcggtgac acccaggtga ccgccgcttt cggcccgcag cacgggctac
    11101 gcggagacaa gcaggacaac atggtggagt ccggggattt ccgcgatcct gtgcacggca
    11161 tcccggtgtt cagcctgtac ggcacggtcc gccgccccac ggcggaaatg atggatacct
    11221 tcgatgtgct gctcgttgac ctacaggatg tgggctgcag ggtctatacc tttgtcacga
    11281 cactccgcta cgtgctcgag gaggccgcga agcggcgcaa atcgctgtgg gtgctcgatc
    11341 ggccgaatcc cgtgggacgg ccggtggaag gtctcacgct ccgacccggc gccgagagct
    11401 ttgtcggcgc gggcccgatg cccatgcgcc atggcctcac gatgggcgag cttgcgctgt
    11461 ggttcgtcac gaggctgcgg ctggatgtcg attgccagat catcccgatg gacggctggg
    11521 agccggccct cgagccgggt tacgggtggc cggcggggcg gcgcacgtgg gtgaatccga
    11581 gcccgaacgc tccgggcctc ggcatggcgc gctgctatcc gggctcggtc atgctcgaag
    11641 gcacaacttt gtcggaaggg cggggaacca ctcgccccct ggaagggttg ggcgcgcccg
    11701 atgtggatgc gcgggcgttg ctggccgaga tgcgcgcggt cgcccccgcg tggatgcgtg
    11761 gctgccggct gcgttcctgt tggttcgagc cgaccttcca caagca
//
LOCUS       DASRHN010000188         7392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1219287, whole genome shotgun sequence.
ACCESSION   DASRHN010000188 DASRHN010000000
VERSION     DASRHN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219287"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /locus_tag="VFB37_07385"
     CDS             <1..2031
                     /locus_tag="VFB37_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22315.1"
                     /translation="VLGPKSDRVLVFSTRRACPSCGSSFGELDPRLFSFNSRHGWCES
                     CCGTGLDMEGLDEEQIEQLGWNEPVGHEPQACEACGGRRLNPIALNVRFRDRSIAALA
                     AESVAGASEFFLRLKLSSREAQIARDLLGEIRARLRFLQRVGLGYLQLDRAAPTLSGG
                     EAQRIRLAAQLGSNLQGVCYVLDEPTIGLHPRDNQILLGALTELSGHRNTLVVVEHDE
                     ETIRRADHLLDLGPAAGVRGGELVGSGTIADLIRNPRSTTGRFLREPLQHPAQPLREV
                     NERTSRLKLERVALHNIKGMDVRIPLGRLVVVTGVSGSGKSTLARDVLYANLRRMLAE
                     GNGSPKGPRNGARNAVPGNGRGSASSGRNTARGLIGCGAIRGLEYVDRVLEVDQTPIG
                     KTPRSCPATYIGFWDDIRRIYEGTTEARIRGYTASRFSFNTAGGRCEACEGQGVKTIE
                     MSFLPDVKVLCDVCGGRRFNSETLAVLWREKSIGDVLTMSVDDAVEFFRAHPRVHHAL
                     KLLQDVGLGYLTLGQQSPTLSGGEAQRIKLVAELSKVRGEVRPGPRQHAPRHTLYVLD
                     EPTVGLHMADVEKLLVVLHRLVEAGNTAVVVEHNLDVMAEADWILDMGPEAGEGGGQV
                     VAQGTPAQIAGKGKDSHTGRMLQEFLRERTRGESGPQVETAIRAQAGSRRKG"
     gene            2042..2344
                     /locus_tag="VFB37_07390"
     CDS             2042..2344
                     /locus_tag="VFB37_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22316.1"
                     /translation="MSHDLVCWKCGASLADLTLPLRRLEECRKCRAELHVCRMCVDYD
                     TRVAKHCREPTAEEVSDKTRANFCDHFKPRPGAYTAPNTVEVNEARAKLEQLFGKR"
     gene            complement(2361..4376)
                     /locus_tag="VFB37_07395"
     CDS             complement(2361..4376)
                     /locus_tag="VFB37_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22317.1"
                     /translation="MTPTPKLTSGATAGADGAGQNVPAAQSGPFVLNLCSSTTPMALP
                     KAEQPELKRFSFFVSRRFEEGRERFRLHMGYFSTLAEAEEWLGAVRDIYPGAWAGEAP
                     GKKLRERAVAAAAAQARHAAGPPPDAATTRPASKEPVAPTARSNLPMPKPAVNTRPAQ
                     QSNPPAIPVARPAPRVPGPEVNVPTLQAAGPSVARATAPVPTDKPATVRPASVARPAG
                     AARLAVSGAVNAAPRTAAVSVPRPAPTQPSQAVRNVPVTTQRVAATARVAPLPAPGAT
                     STPLTAPAARPAVPLAAGPTRGNSQAGIPARNLTQPPQIKPSAAPVRVAPVRVEPVRT
                     AAAAAGVSRPTGVPRPALKSANKPLLPNSNVREVLAALSESPQESTGETRMMPAPVMP
                     PAQPADDAVFTDTQVLKFLEMRRADGNTDPGVEAETGAISLLKPDDTGTRRALKQAVV
                     DNAAVSFAVQLQWSVQPIELAKVPPLAIFSAYTLYTVEGSRDGRRWYGLRLGFFSDAI
                     SAKQVAYYVRSEFTSVAVVPVSPQEHTRASDVDKKGSTRIGSPIKTRPEAQDEFKLID
                     PEDVSASAPAAVRKAGKPQPASSPVQAAAPAKSGAKGSRVRARERRGPQTLEETLEIL
                     GASELEIDAGTGETLNESGVRHLRVEVQKNTPFSRLLERLSERVRKS"
     regulatory      complement(4472..4557)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4733..>7392)
                     /locus_tag="VFB37_07400"
     CDS             complement(4733..>7392)
                     /locus_tag="VFB37_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO22318.1"
                     /translation="LIKSRLGVAETIVEAFTANDVGSESSTLERQAGNSRVFKSVVIT
                     DSEGLLAGGEAVLRPSPPQLLALEAGQTVLMPVALEGQLTSVFMARHITAAGSSRLAY
                     FELAPDWLWSEMDDLRGTTLVVIDADGRLLHGNGALTPDTSRMFAEHITQSGERGGSL
                     DSLSWQDGGKPWRGVLKHIPLVNERITAIPWGVVAYTTESSFLARSKAVWRILPVALL
                     ALLLCAVLASGYLGRRYFPVWAALQKGLAALPTRRFETLPVAGSDEPRELIETFNRCV
                     ISLRDQYRALETLGEIDKLLLGSADLEQVLEGILSRVQAVTRCHCVGITLRDADAPGR
                     GRVYLAATGLVGLPVTRVELDPDMLTTLAAESAGLTIARCEEARHSFLSPLSEAGAEF
                     FWVCPVNVAERIEAILAVGYREPPAADPLLARSGSQFAERLAVALSKSAHDERLYRQA
                     HFDPLTSLPNRILFRDRLSQEIANATAGLSRGALLYIDLDHFKRVNDSMGHGAGDQLL
                     GIVAQRLRSCVKEGDTVARLGGDEFTVILRNVADPDSARTIGERIIESLQLPVNIAGR
                     DHFVCASIGITLFPDDGSSIDDVMRNADMAMYRAKDLGRGRIMFFDPRMNSRGVLPTE
                     TGLQRALRRREFSLFYQPQFTVVDGTLAGVEALLRWQTAREGMRQPGEFVPAAEESGL
                     IVDIGGWVLEAACAQLAAWRDQNIAPPRLALNISAQQLKHEEFPKLVRRALDKYGLPA
                     AWLELELTESVFADRAAGAALGRLAQLGVHLALDDFGTGYSSLSYLRQHPIGTVKIDR
                     SFLEEVPQNPASATLVETIVVMSHALGKRVVAEGIETAEQLAFLRERNCDVAQGFYLS
                     RPLSADVATELLQVRAHAGDKSGGIREAG"
BASE COUNT         1258 a         2411 c         2463 g         1260 t
ORIGIN      
        1 gtgctcggcc cgaaatccga ccgcgtgctg gtgttctcga cgcgacgcgc atgcccctcc
       61 tgtggcagca gcttcggaga gctggaccca cggctgttct ctttcaactc cagacacgga
      121 tggtgcgaga gctgctgcgg caccggcctg gatatggagg gactggacga ggagcagatc
      181 gagcagctgg gatggaacga gccggtcggg cacgagccgc aggcgtgcga ggcttgcgga
      241 gggcggcggc tcaatcccat tgcgctcaat gtgcgcttcc gtgaccgatc catcgccgca
      301 ctggccgccg agtccgtggc gggggcgagc gagttctttt tgcgcctcaa gctcagctcc
      361 cgggaggcgc agatcgcacg tgacctgctg ggagagattc gtgcgcggct gagattcctg
      421 cagcgcgtgg gtctgggcta cctgcagctc gatcgagccg cgccgacatt gtccggcggt
      481 gaagcgcagc ggattcgctt ggccgcgcag ctcggatcca atctccaggg ggtgtgttat
      541 gtgctcgatg agccgacgat cggcctgcat cctcgtgaca accagattct gctcggggcg
      601 ctgaccgagc tctcgggcca tcgcaacacg ctggtcgtcg tcgagcacga cgaggagacc
      661 atccggcgcg ccgaccacct gctcgatctc ggtccggctg cgggcgttcg cggcggagag
      721 ctggtcggct ccggaacgat cgcggatctg attcgcaatc cacgctcgac cacgggtcgt
      781 ttcctgcgtg agcctctgca gcatccggca cagcccctgc gcgaagtcaa cgagcggacc
      841 tcacgtctga agctggaacg ggtggcgctt cacaacatca agggaatgga cgtgcgcatt
      901 ccgctgggac gactggtcgt ggtgaccggc gtctccggat ccggcaagtc gacgctggca
      961 cgcgatgtgc tctatgccaa tctcagaaga atgctcgcag aaggcaatgg ctcgcccaaa
     1021 ggtccccgca acggggcgcg taatgcggtc cccggcaatg ggcggggatc ggcgagctcc
     1081 ggtcgtaaca ccgcgcgcgg gctgatcggc tgcggtgcga tccgcggact cgagtacgtc
     1141 gaccgggtgc tggaggtcga ccagacaccc atcggcaaga cgccgcgctc ctgtccggca
     1201 acctacatcg gattctggga cgatatccgg cgcatctacg aagggacgac cgaagcgcgc
     1261 atccgcggat ataccgccag ccgcttctca ttcaacacgg ccggtggacg atgtgaggcc
     1321 tgcgaaggcc agggcgtcaa gaccatcgag atgagcttcc tgccggacgt gaaggtactc
     1381 tgcgatgtct gcgggggccg tcgcttcaac tcggaaactc tggcagtgct ctggcgggaa
     1441 aagagtatcg gcgatgttct gaccatgagc gtggatgacg ccgtcgagtt cttccgtgcc
     1501 caccctcggg tgcaccacgc gctcaagctg cttcaggacg tcggcctcgg ctatctgacg
     1561 ctcggtcaac agagtcccac cctttcgggg ggcgaggcgc agcgcatcaa gctcgtggcg
     1621 gaattgtcca aggtgcgcgg cgaagtacgg ccaggccctc ggcaacacgc cccgcgacac
     1681 actctctacg tactcgatga gcccacagtg gggctgcaca tggccgatgt cgagaagctc
     1741 ctggtcgttc tgcatcggct ggtcgaagcc ggcaacacag cggtcgtagt ggagcacaac
     1801 ctcgatgtca tggccgaggc ggattggatt ctcgacatgg gccccgaggc cggcgaaggc
     1861 ggcggccagg tcgtcgccca gggcaccccg gcccaaattg ccggaaaagg caaggactct
     1921 cacaccggac gcatgctgca ggagttcttg cgcgagcgga cccggggcga gtccgggcca
     1981 caagtcgaga ccgccatccg cgcgcaggcc ggctcccgcc gcaaggggta gactcgctcc
     2041 cgtgagccac gatctcgtct gctggaaatg cggcgcctcc ctggcggatc tgacactgcc
     2101 gttgcggcgg ctcgaggagt gccgcaaatg ccgcgcggag ctgcacgtct gccgcatgtg
     2161 cgtggattac gacacgcggg tggcgaagca ttgccgcgag cccacggccg aagaggtcag
     2221 cgacaagacc cgggccaact tctgcgatca tttcaaaccg cggccgggag cctatacggc
     2281 acccaacaca gtcgaagtga acgaggcgcg cgccaagctg gagcagctgt tcggtaaacg
     2341 ctagcgcgcc ggtgcgccga tcaggacttg cgcacccgct cgctcaatcg ctccagcagc
     2401 cgcgagaacg gtgtgttttt ctgcacctca acacgcaggt ggcgcacgcc gctctcgttg
     2461 agcgtctcgc cagtgccggc atcgatctcg agctcactcg cgcccaaaat ctccagagtc
     2521 tcctcgagag tctgtgggcc acgtcgctcg cgggctcgca cccgggaacc tttcgcgccc
     2581 gatttggcgg gggcagccgc ttggacgggg gaacttgccg gctggggctt gcccgctttg
     2641 cgaaccgccg cgggcgccga ggcggagacg tcttccggat cgatgagctt gaattcgtcc
     2701 tgcgcctcgg gccgtgtctt gatgggcgat ccgatccgcg tggagccctt cttgtccaca
     2761 tccgaagcgc gtgtatgttc ctgcgggctc acgggcacga ccgccaccga ggtgaactcc
     2821 gagcggacgt aataggccac ctgcttcgcg gaaatcgcat cgctgaagaa tccgagccgc
     2881 agaccgtacc acctgcgccc gtcccggctt ccttcgacgg tatagagcgt ataggcactg
     2941 aagatggcca gaggcgggac cttggccagt tcgatgggct ggacggacca ttgcaactgc
     3001 acagcaaagg agacggcggc attgtccacg accgcctgct tcagcgcccg cctggtgccc
     3061 gtgtcgtccg gcttcagaag agatatggcg cccgtctcgg cttcgacgcc cgggtcagtg
     3121 ttgccatcgg cgcggcgcat ctcgaggaac ttcaacacct gagtgtcggt gaacacggcg
     3181 tcgtctgccg gctgtgcggg gggcatcaca ggcgcgggca tcatgcgcgt ttcgccggtg
     3241 gactcctgcg gcgattcact cagggccgca aggacttcgc gcacgttgga gttgggcaga
     3301 aggggtttgt tcgctgactt gagcgcaggc cgcggtacgc cggtcgggcg cgaaacaccc
     3361 gcagctgcag cggcggttcg cacgggctcg acccgcacgg gcgcaactcg caccggcgcg
     3421 gcactcggct taatctgcgg cggctgagtg agattgcggg cgggaatacc ggcctgacta
     3481 ttgccacgcg tgggaccagc agccagcggc actgctgggc gcgctgcggg cgccgtcaac
     3541 ggcgtggatg tggctcccgg agcgggaagc ggggccactc tcgcagttgc agcgacacgc
     3601 tgcgtcgtaa cagggacgtt tcgcactgcc tgcgacggct gagttggcgc aggccggggt
     3661 acggagaccg ctgccgtgcg cggagcggcg ttcacggctc ccgacaccgc gagtcgggcc
     3721 gctccagcgg ggcgtgcaac cgaggcgggt ctcaccgtcg caggtttgtc ggtgggcacc
     3781 ggcgcagtgg cgcgcgcaac gctcggccca gccgcctgga gcgtgggtac gttgacttcg
     3841 gggcccggaa cgcgcggagc tggccgcgca acgggaatcg caggaggatt cgactgctgt
     3901 gccgggcgcg tgttgacggc gggcttgggc attgggagat tgctgcgagc ggtcggcgcg
     3961 accggttcct tgctcgccgg gcgcgttgtg gcggcgtccg gcggcggccc tgccgcatga
     4021 cgcgcctggg ctgctgcggc agcaaccgcc cgctccctca gcttcttgcc gggcgcctca
     4081 cccgcccagg ccccgggata gatatcgcgg accgcgccga gccattcctc ggcctctgct
     4141 agggtcgaaa agtagcccat gtgcagacgg aatctctcgc gcccctcttc gaagcgtcgg
     4201 ctgacgaaga agctgaagcg cttgagctcg ggctgctccg ccttgggcag cgccatgggg
     4261 gtcgtggatg agcacagatt gagtacgaag ggaccgctct gtgccgccgg cacattctgg
     4321 cctgcgccat cggcgcctgc ggtggcgcca gatgtcagct ttggtgttgg tgtcatagac
     4381 cgtccccgga ccgatgcgta ctcgctgtac ggcccacagg gaggcggatt cgacgggctg
     4441 ggagtgcact cgatcgcgcc aggcgcgcga tcgtggcagc tccgccgtca taggaatccg
     4501 tcctttagac cggtagcttt gcgtccccgt ctttcgacgg gtttgccctt atcacaggga
     4561 ccaagtgtcg aagcgtcgcg gatgacagtc aagagcttat attgtgttca gcatctcaac
     4621 cccgcggcac atattcacgc gctccgcaat atgtctcaac ggagcgacgc gagccggaac
     4681 ggcgtctcac tttgcgcgcg ctgcgcagcg tgagctctac agtggcagtg ggtcagcccg
     4741 cctcgcggat cccgccgctc ttgtcgccgg cgtgcgcccg cacttgcaac agttctgtcg
     4801 caacatcggc tgaaagtggc cgcgaaaggt agaagccctg cgcgacatcg caatttcgct
     4861 cgcgcaggaa ggcgagctgc tcggcagtct cgatgccttc ggcgaccaca cgcttgccga
     4921 gtgcgtgcga catcacgacg atcgtctcga cgagcgtggc tgatgcagga ttctgcggca
     4981 cttcctcgag aaagctgcga tcgatcttca ccgtgccgat gggatgctgg cgcagatacg
     5041 agagcgatga atatcccgtc ccgaagtcat cgagcgccag atgcacgccc agctgggcca
     5101 ggcgcccgag cgcggcgccg gcggcacggt ccgcaaacac actctcggtc aactccaact
     5161 cgagccacgc tgcgggcagc ccgtacttgt cgagcgcccg gcgcaccagt ttcggaaact
     5221 cctcgtgttt gagctgctgg gcggagatat tgagcgctag ccgcggagga gcgatgttct
     5281 gatcccgcca ggcggcgagt tgtgcgcatg ccgcctcgag tacccagccg ccgatgtcaa
     5341 cgatgagccc gctctcctcg gccgctggca cgaactcgcc tggctggcgc atcccttcgc
     5401 gtgccgtctg ccaccgcagc agcgcctcta cgccagcgag cgtcccatcg accaccgtga
     5461 actggggctg atagaacagc gagaattccc gccgtcgcag cgctcgctgg agccccgtct
     5521 cggtgggtaa cactccgcga ctgttcatcc gtgggtcaaa gaacatgatg cggccgcgcc
     5581 cgaggtcctt ggcgcggtac atggccatgt ctgcattgcg catcacgtcg tcgatgctgg
     5641 agccgtcgtc gggaaacaac gtgatgccga tgcttgcgca cacgaaatga tcgcggccgg
     5701 cgatgttgac cggcagctgc agcgattcga tgatgcgctc accgatggtt cgggcggaat
     5761 ccgggtcggc aacgttgcgc agaatgacag tgaattcgtc gccgccgagg cgtgcgacgg
     5821 tgtcgccctc cttcacacac gagcgcagtc gctgcgcgac gatgcccagc agctgatcgc
     5881 cggctccatg tcccatggag tcattcacgc gcttgaaatg atccagatcg atgtacagga
     5941 gcgcgccgcg gctgagaccc gccgtggcgt tcgcgatctc ctgcgacagc cgatcgcgaa
     6001 acaggatgcg gttgggaagg gaggtcaacg gatcgaagtg cgcctggcga tacaggcgct
     6061 catcgtgagc gctcttggat agcgccaccg cgagccgttc cgcaaactga ctgccactgc
     6121 gcgccagcag cgggtccgct gccggcggct cccgatagcc caccgccaga atcgcctcaa
     6181 tccgctcggc tacgttcacc ggacagaccc agaagaactc cgcccccgcc tcgctcaggg
     6241 ggctgaggaa gctgtggcgc gcttcttcac agcgtgcaat ggtcagcccc gcgctctcgg
     6301 cggcgagtgt agtcagcatg tctggatcga gctcgacgcg cgtgaccggc aggcccacca
     6361 gtccggtggc ggccagatag acgcggccgc gccccggcgc atccgcatcg cgcagcgtga
     6421 tgccgacgca atggcagcgc gtgaccgcct ggactctcga gaggatcccc tccaggacct
     6481 gctcgaggtc agcggatccc agcagcagct tgtcaatctc cccgagcgtc tccagagcac
     6541 ggtattggtc ccggaggctg atgacacaac gattgaacgt ctcgatcagc tcgcgcggct
     6601 cgtcgctccc ggccaccggc agcgtttcga agcggcgcgt cggcaaggcc gcgagtccct
     6661 tttgcagcgc cgcccacaca ggaaagtagc ggcgcccgag atacccgctt gccaatactg
     6721 cgcacagcag cagtgcgagc agcgccaccg gcaagattct ccagaccgcc ttggagcgcg
     6781 ccaggaaaga gctttcggtc gtgtaagcca ccacgcccca gggaatcgca gtgattcgct
     6841 cgttgaccag cgggatgtgc ttgaggacac cccgccaggg cttgccgccg tcctgccacg
     6901 aaaggctgtc cagcgatcca ccccgctctc cggattgcgt gatgtgctcg gcaaacatcc
     6961 tgctcgtgtc cggagtcagg gcgccgttcc cgtgcaagag gcggccgtcg gcatcgatca
     7021 ccacgagtgt cgtacctcgc agatcgtcca tttccgacca gagccagtcc ggggcgagct
     7081 cgaaataggc caggcgactg ctcccggcgg cagtgatgtg acgcgccatg aatacgctgg
     7141 tgagttgtcc ctccagcgcc acgggcatga gcacggtctg gccggcctcc agggcgagca
     7201 attgcggcgg gctggggcgc agcaccgctt ccccgccggc aagcagtcct tcgctgtcgg
     7261 tgatcacgac cgacttgaag acccgggagt tgcccgcttg cctctcgagc gtggagcttt
     7321 cggagcccac gtcgttggcc gtgaaggcct cgacgatcgt ctccgccacg ccgagacgcg
     7381 acttgatcag gt
//
LOCUS       DASRHN010000189         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_393970, whole genome shotgun sequence.
ACCESSION   DASRHN010000189 DASRHN010000000
VERSION     DASRHN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393970"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFB37_07405"
     CDS             <1..322
                     /locus_tag="VFB37_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO22319.1"
                     /translation="ILRILRGEGAPLPILEIAQRMVTVVPGITGLIDRLEQAGLVERR
                     RCPEDRRVVYVALRDKAAELLAQLDEPVLQLHKRLMGHLSEEELRELIRLLEKAREGF
                     DAAG"
     gene            395..1147
                     /locus_tag="VFB37_07410"
     CDS             395..1147
                     /locus_tag="VFB37_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZO22320.1"
                     /translation="MKVRPSAERGHFDHGWLNTYHTFSFADYYDPEHVDFGPLKVMNE
                     DRIRPGKGYSQQRQDTEVLTYLIEGELRHQDSLGNEVMLRAGGLQCMSAGAGLNHSEH
                     NPSASQETHLLHICVAPATAGLAPSYAQRHFSPEEKRGTLRLLASPDGQDGSLSINQD
                     ARLYGGLFHESQRLEFESGNGRRAFLHVVRGSIALNETRLNAGDGVKITPAEGIVLQH
                     GRDAEVIIFDVPGEVAPAKRKPVPRPKSKSTE"
     gene            1284..1964
                     /locus_tag="VFB37_07415"
     CDS             1284..1964
                     /locus_tag="VFB37_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22321.1"
                     /translation="MRVLVVEDDSLVADAIRRGLSMAGFAVDCASDAQQAANALGAEH
                     FDLTVLDIALPGVDGLTFLRRLRNSGTQLPVLILTARETLATKVKALDLGADDFLVKP
                     FEQAELAARCRALIRRSNLNSSGQLTLGRLRIDITGRQLYVGGEPVELTGREWSIIEC
                     LALNSGRIVSKERLQQAVANWDEAITPNAVEVHVSRVRAKLGESAVLRAVRGLGYRLD
                     EARSLADQ"
BASE COUNT          392 a          639 c          691 g          385 t
ORIGIN      
        1 catcctgcgc atcctccgcg gcgagggggc gccgctgccg atcctggaga tcgcccagcg
       61 catggtgacg gtggtgcccg gcatcaccgg cctgatcgac cggctggagc aggccggcct
      121 ggtcgagcgg cgccgctgcc cggaggaccg ccgcgtcgtt tacgtcgccc tgcgcgacaa
      181 ggcggcggag ctgctggccc aactcgacga gccggtgctg caactgcaca agcgcctgat
      241 gggccacctc agcgaggagg agttgcgcga gctgattcgc ctcttggaga aggcgcggga
      301 gggattcgac gccgccggct gaggcgctgt tttttcggcc ggttaattta aacatcaact
      361 attgacattt gatcgaaaga gggagggtgt tatgatgaag gttcgaccga gtgcggagcg
      421 cggccacttc gaccacgggt ggctgaacac gtaccacacg ttctcgttcg cggactacta
      481 cgatccggag cacgtcgact tcggtccgtt gaaagtcatg aacgaagatc gcatcaggcc
      541 gggcaagggc tacagccagc aacgtcagga tacggaagtg ctcacctacc tcatcgaagg
      601 cgagctgcgg catcaggact cactgggcaa cgaggtcatg ttgcgggctg gaggcctgca
      661 atgcatgagc gcgggggcgg ggttgaacca cagcgagcac aacccttccg catcgcagga
      721 aacgcacctg ctgcacatct gtgtagcgcc ggccacagcg ggtctcgctc ccagctacgc
      781 gcagaggcat ttctccccgg aagagaagcg gggcacgctg cgtctgctgg catcccccga
      841 tggtcaggac ggctcgctga gcatcaacca ggatgcccgg ctgtatggcg gtctgtttca
      901 cgagagccaa cgtctggagt ttgaatcggg caatggcagg cgtgccttcc tgcatgtggt
      961 gcggggctcg atcgcactca acgaaacccg gctgaacgca ggcgatggtg tgaagatcac
     1021 acccgccgag ggcatcgtgc tccaacatgg acgcgatgcc gaggtaatta ttttcgacgt
     1081 ccccggggag gtagcacctg ccaagcggaa accggtgcct agacctaagt ccaagtccac
     1141 ggagtgaaca ccgcacgccg gtggctccac gcgaagaccc ctccacactg cagcaaactg
     1201 cgggcgggcg tgagtattca gctctccgct gaagtacact gccgcactgc aaaaaacgtc
     1261 gaggtctttc ttacagggaa tgtatgcgcg tcctggtggt cgaggatgat tcgctggttg
     1321 ccgatgccat ccgtcgtggg ttgtccatgg cgggcttcgc cgtggattgc gcatccgacg
     1381 cgcagcaggc cgcgaatgcg ctcggcgcgg agcacttcga cctgacggta ctcgatatcg
     1441 cgctgccggg agtggatggg ctgacatttc tgcgccggct gaggaattcg ggcacgcagc
     1501 ttccggtgct gatcctgacc gcgcgcgaga cgctggcgac gaaagtgaag gcgctcgatc
     1561 tgggtgccga tgatttcctg gtcaagccct tcgagcaggc agagctcgcc gcgcgctgcc
     1621 gcgccctgat acgacgttcc aacctcaact ccagcggtca gctgactctc gggcgtctgc
     1681 gcatcgacat caccgggcgg caactgtatg tcggcggtga gcctgtcgag ctgacaggcc
     1741 gtgagtggtc gattatcgag tgcctcgcgc ttaactccgg tcgcatcgtc agcaaagagc
     1801 gcctgcaaca ggcagttgcc aattgggacg aggccatcac accgaacgcg gtcgaagtac
     1861 atgtctcgcg ggtgcgtgcc aaattgggag aatcagctgt gttgcgcgct gtgcgcgggc
     1921 tggggtacag gctcgacgaa gccaggagcc tggctgatca atgatcgcct gctgcgccct
     1981 cgcgacggcg ctgattgatc agacaggctc ctgatgagcc cgagcgcgct gcccgcgaca
     2041 tcgatccgcc gacgcctgct ggcctttctt ctgcctccgc tgattctgct catgctggca
     2101 ggcgtct
//
LOCUS       DASRHN010000190        11711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1712153, whole genome shotgun sequence.
ACCESSION   DASRHN010000190 DASRHN010000000
VERSION     DASRHN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712153"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFB37_07420"
     CDS             complement(<1..971)
                     /locus_tag="VFB37_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZO22322.1"
                     /translation="MRVLTNAAWLSFSRASTDALSFVLFAVIARTFGPAGSGEYSYAF
                     AAGMLFALFATCGLEEFGIRQYARAAAADRPRLWADLLTMQCVQIVLGALAFAILGLT
                     GIIHPTRWTVVLEIATYLTGWTVARTLFVPSMAAQLMVTPAVTDLSCRLAGIACALVF
                     AVWLKLPLPALLIGFPVAGVALVALALRNAVRRGAALRLHPSWHALAATARGVAPFGG
                     VDLLGQFYARTDLLLIAYFLGTADVGLYATDIKFVEVGLLPLFMLGSAAYPLLSRHAG
                     RDPDAFTRCGRDLVRIVFFCSGWLAVGIFCLVPLLLVPLFGEKFRPAA"
     gene            1336..3411
                     /locus_tag="VFB37_07425"
     CDS             1336..3411
                     /locus_tag="VFB37_07425"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO22323.1"
                     /translation="MKIGIVGSGMISGHHLTAASRYPGAVIVGIADRDISRAKAQAQR
                     FGASKTCTDLSELLALKPDVIHILTPPASHAALTVQALQGGAHVYVEKPMALSLADCE
                     LMSHAARAAARELCVGHCWLYSPAMLRAQELIASGATGEVLQATSSFNFDVRRNASYG
                     DGHWASDLPGGLAEDLAIHPASILIRLLGAPRKVVAVTRTAASIPNAGTADARALIDA
                     EGGIGTLSVSLRARPDMGLVDISCARMLLRINISSMTLTMHRELPVPQKIGRGLGNLD
                     IATQLFTGTAGVMWKLVRKKVDGSYGIVPLIHAFYRAIEAGQPAPVSAEEGTQSVAMM
                     RAMWPEAPSAAAEQPARPVVISSAPAGKRALVTGATGFVGSHLVRELLSRGYAVRILA
                     RNAGRAASLAKAGAEVRLGDLGQPETLHGIAEGMDVVFHLGSAMRGNSEAFERVDLGG
                     THALLAEAQRAGVRRVVFAGTLACYPLGQQRDGAVINEQCPFDETGLLGNYARAKVRA
                     ETAVLEAARSGKMEGVIVRLGLVCGEGASIFPAHVCQKIARNLVVLYGDGRVPLPLTF
                     IDNAVHALVLGAEVPGVSGESFNIVDDDVMTQQQYLELLRRYMGGAPRVVRMPRLAYY
                     ALGLMTEIAAALRKKEPATTRYRIRNRLVPVRWDCSKAQRVLKWRPQVPLLEGLSRTF
                     TAHAGRHAG"
     gene            3401..4369
                     /locus_tag="VFB37_07430"
     CDS             3401..4369
                     /locus_tag="VFB37_07430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO22324.1"
                     /translation="MLDSSAPKLSVIVPFYNEQESIRPMYEAIVAGVAPLMVPFEMVF
                     VDDGSKDDTLVRARAIAENDPRVRVVKFRRNYGQTAAMAAGIEYACGEVLVTMDGDLQ
                     NDPSDIGLFLQKIDSGYDIVVGWRHQRQDKLVSRKIPSRIANWLIGKVTGIPIKDNGC
                     SLKAYRAKVIKPIPLYSDMHRFIPAMASIAGPRIAEVKVKHHARRFGSSKYGLSRIYK
                     VLLDLMVIKTLASFAARPLLWFGLLSLPLLLAGTAVLGYALIRALLDGAPPSLPMAGA
                     GIIFLTSAFIMLCSGAMGELIYNLGDMRDHEFACLTQQLRTRAARN"
     gene            4382..5386
                     /locus_tag="VFB37_07435"
     CDS             4382..5386
                     /locus_tag="VFB37_07435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO22325.1"
                     /translation="MTNRKVQLSVIIPVGRRHADIGELYAEYRAGIDPLRLTYEFIFV
                     LDGPNAQVLSALEALIDAGDHNITLVCLNRSFGESTALMAGFERSSGDLILTLPAYHQ
                     VEGAGLAKLVSGLDCADVVVGWRWPRAGGMLERLRRNAFHRMVSLVTGMRLHDLGCGA
                     RAMKRGVLEELSLYGDRHRLLAILADRQGFRVREVELTQSNRDRFDGIYRPGDYTHLV
                     LDVFTVFFLVRFTKKPLRFFGALGATMFGVGALLVLYLVIDRLAFGTSLADRPALLLS
                     ALVVVLGLQLFALGLLAELIIFTHARQIKDYRTAEIIQYPSPPARAAREAAPEKAALR
                     "
     gene            5396..7432
                     /locus_tag="VFB37_07440"
     CDS             5396..7432
                     /locus_tag="VFB37_07440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO22326.1"
                     /translation="MGAQRGYARDWILWALALLTGCCLIAGILARFAGLGRWPLTVDE
                     YYVAQSVQNILREGIPHYACGGLYVRGLALQYVSAGLQLAGLSAELAPRLVAALSSLL
                     ALPAVYLLGRRVGARDIGLLAVAILAISVWETEVARFGRMYAPFQAVFVWYLVCFLAY
                     TLDRSRRALVGMLVLSVLGVLVWEGGVFLALANLLPPFIRKPSGRFEARDWKYLAGCA
                     LLVLPSYWLATADLRTRGAEPTLPADYQEPAATALSRLDSANLPLSSLHLHPMWALAV
                     LLPVLLALFAVVRLARAYAGSLRAAAGTDALAPPQALGALALLGCSAALGCALMAQFG
                     LAFALVLLMGLLYILPWSALFRREMWPFHAAILLSLVLWIGFGLSTPDWHAPGLNRLH
                     VDFLLGYEFVRFPDVVRQIAAPWGHSVPVLSAALLVLIGAACIHACMLPAETGAAERV
                     LLALLVIFVLAAGASHPPRQETRYVFFLYPLAIVLATMSVSRIAHELFGARRLAAAAT
                     VVLALLGFALTEDFRPRHLWNIDTEAVTFRIGMPPREAAHYQERSDIRSAARWLAAHA
                     SPGRDIVINSFPGVDFYYPHADFYYMEESDPRFESWSCRRGRLERWSNLPLLHSVGAL
                     QSELASGRRVWLVLETSRLPQLESAFAAGTWQLEWSSPEHDIAILSSHARTPTQ"
     gene            7878..8462
                     /locus_tag="VFB37_07445"
     CDS             7878..8462
                     /locus_tag="VFB37_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZO22327.1"
                     /translation="MLHLFRVARLPLLAAACLLGAGLTNPAFAEIPTTDYHIHPGDKI
                     LIGLYDDPKMQPVEITVAPDGKLAFPLIGVISAGGKTVDQLRVEMETKLKKFVSEPVV
                     TVAVTQVLGNVAYVIGQVNKPGSFIMNPTINVLQALALAGGGNPYAKMDGIIIIRSSS
                     GGQRVLNFRYGQVASGRNLEQNVNLEAGDVVVVP"
     gene            8443..9666
                     /locus_tag="VFB37_07450"
     CDS             8443..9666
                     /locus_tag="VFB37_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22328.1"
                     /translation="MSSSYRSTAVVAAGACSLSCWLAAQAQEVYWQPQVSLTGTYNTN
                     VELSPLASDKVSSQGYIGDAASLIGIATPTSQTTLRPDIRYEYYPQDKSVDRLEGFLD
                     LNSQFSWQRDRFTMLGRADRLTDLSAEQPEAQFNQVNPGLPTTPTTGRVIANETRNEI
                     YLVPDYSHALTPLTNLGLSATYQHLSYTPDDAFDHVDYNYYQGRPFVDYQYNERTLFT
                     VGVFASHFSAMNIDSTSKDYGGDIEMRYNWSPIFRSTMEVLYQRTTLDQVTPMPFFAH
                     TNNWGANFDTQYLGQTNRLKASIGRSILPSSAGSLYNTDLVRIEYDHDFTERLTFTGA
                     AMYEKIGTLASNFASDTRSYSSSGIFLRWMMTPVLFIKGGYSYIWQKYQLDPNSAANN
                     QAMLQFGYIARGRER"
     gene            9666..11216
                     /locus_tag="VFB37_07455"
     CDS             9666..11216
                     /locus_tag="VFB37_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22329.1"
                     /translation="MDTQENTGGVNSYMGILRRRRVYVGTIAPLITFAAIYLAFTLPP
                     EYQSTATILIEPSVVNKDVVAPTVASYSNQQIEIVEGRVLKLDTLKALVKSYDPYPED
                     KTMDLTQKAQRVLQSTTLERVDPVTMKPDQDANAFSLHYNNPDPERASETAKRLAQAF
                     LTYNQRSREQAAQDATLFLKQQAEAVSQQMREIDEEINKFKNAHGDALPEDMARNEAA
                     IDRDQHDLDSLQQEILRVQEKEGLLSVELSQISPNMISQAGDLTDVATVRTKLAEAEQ
                     RYTPDHPEVKRLKRALQDLMAAQGTATKGGIVSNANNPQYLSTASELQSARKELDSLH
                     GQADRLRGEINKYEELLRRTPGVEREYSDITRRRQSAQTAYAQIQDKLQHAQLAESFE
                     SEEQGEKFVLLRAPIPAKLPVYPNRIGLILLGFILGGALAAIAVAIAESADSTVRSVR
                     DFPEAEDAPILASIPQIDNSSDRRRNRLRFATWVAAYTAAVLVIGVAVVSGLKSTTTT
                     PVAQTQAQ"
     gene            11243..>11711
                     /locus_tag="VFB37_07460"
     CDS             11243..>11711
                     /locus_tag="VFB37_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22330.1"
                     /translation="MTIIESALERAKALKSGRPALHTPAAPAAVHRPPRRVLDGPAPS
                     VLFQPQRVALDPRAARENRLLLNSSVQEDRPAIAAYGMLRTRLLHRARAKGWTTIGVT
                     SPAPQDGKSLTALNLSLSLAREQNNTVALIDLDMRNPSMCRCLGIEPPVELRDF"
BASE COUNT         2010 a         3853 c         3792 g         2056 t
ORIGIN      
        1 gcggcgggcc ggaacttctc gccgaacagc ggcacgagca gcaagggcac cagacagaag
       61 atcccgacag cgagccagcc ggagcagaag aatacgatcc gcaccagatc gcggccgcaa
      121 cgcgtgaagg catccgggtc gcgtccggcg tggcgactca gaagcggata ggccgcggag
      181 ccgagcatga acagcggtag caaccccact tcgacgaact tgatgtcggt ggcgtagagc
      241 ccgacgtcgg cggtgccgag gaagtaggcg atgagcagca gatcggtgcg ggcgtagaac
      301 tgaccgagca ggtccactcc gccgaagggc gcgacacccc gggcggtggc ggcgagggca
      361 tgccaactcg ggtgcaagcg cagcgcggca cctcgccgca ccgcattccg caaggcaagg
      421 gccaccagcg caacgccggc caccggaaac ccgatcagca gcgcaggtag cggcagcttg
      481 agccacacag cgaacacgag tgcgcaggcg atcccggcga ggcgacagga taggtccgtg
      541 accgcgggcg tcaccatgag ctgagcggcc atcgagggca cgaatagagt gcgcgccacc
      601 gtccaacccg tcaggtaggt ggcgatctcg agcaccaccg tccagcgggt ggggtgaatg
      661 atgccggtca gtccgagtat cgcgaacgcc agcgcgccga gaacgatttg cacacactgc
      721 atggtcaaca ggtcggccca cagacggggg cgatccgcag cggcggcgcg cgcgtattgc
      781 cggatgccga attcctcgag gccgcaggtg gcaaacaggg caaacagcat ccccgcggcg
      841 aaggcatacg aatactcgcc gctgcccgcc gggccaaatg tccgcgcgat cactgcgaac
      901 aacacgaagc tcagcgcatc ggtcgacgct cgtgagaacg acagccacgc ggcgttggtg
      961 aggaccctca tgttgggaag cagccgcgct tcgctgaccg aagtgtcacg gaatcgctgc
     1021 ggctcgaggc cggacggggg cgaactgagc cgagatgtcc attgtgtgaa tcagtacctt
     1081 gtcgcgggag ttgatttgtt gttaccgggg tcactggggt ccgtcatccg cgccgcccac
     1141 gcggatttgt caaaatgttt cctgggccgg aattaagtca gccgggctgc gggtccagcg
     1201 gagcgaagcg aacccgcggc acggatactt gctaaactag ccgccgggcg caatggcgcg
     1261 cgagcgcgcc atcaactggc cttcagacca gcagtcgacc cggcgaagta gaacctgagc
     1321 gagcatttat accaaatgaa gatcggtatt gtcggaagcg ggatgatctc gggtcaccac
     1381 ctgacggccg cgagccgcta cccgggagcg gtcatcgtcg gcatcgcgga tcgcgatatc
     1441 agtcgcgcaa aggcccaggc gcagcgcttc ggtgccagca agacctgcac cgacttgagc
     1501 gagctgctgg ccctcaagcc tgacgtcatt cacatcctga ctccgcccgc atcgcacgcc
     1561 gccttgacag tgcaggcgtt gcagggcggt gcccacgtct atgtcgagaa gccgatggcg
     1621 ctgagccttg ccgactgcga gctcatgagc catgcggcgc gggcggcagc ccgggagctg
     1681 tgcgtcggcc attgctggct gtattccccg gcgatgctgc gcgcacagga gctcatcgct
     1741 tccggcgcca cgggtgaggt gttgcaagcg acatcttctt tcaacttcga cgtgcgccgc
     1801 aacgcgagct atggcgatgg ccactgggcc tcggatctgc ctggcggcct tgcggaggat
     1861 ctggcgattc acccggcctc gatcctcatc cgcctgctcg gagcgcccag aaaagtcgtc
     1921 gcggtgaccc gcaccgccgc ctcgattccc aatgcaggca ccgccgatgc tcgcgccctg
     1981 atcgatgcgg agggcgggat cggaacgctg tcggtctccc ttcgggcgcg tcccgatatg
     2041 ggactcgtgg acatctcctg tgcccgcatg ctgctgcgga tcaatatctc gagcatgacg
     2101 ctcacgatgc atcgtgagtt gccggtcccg cagaagatcg gtcgcgggct cggcaatctc
     2161 gatatcgcga ctcagctctt taccggcacg gccggcgtca tgtggaagct ggtgcgcaag
     2221 aaggtcgatg gctcctacgg gatcgtcccg ctcattcacg cgttctaccg agcgatcgag
     2281 gccggccagc ctgcgccggt cagtgccgag gaaggcaccc agtccgtcgc gatgatgcgc
     2341 gcaatgtggc ccgaggcgcc gagcgccgcc gctgagcagc ccgctcgccc ggtcgtgatc
     2401 tcgagcgccc cagcgggcaa gcgcgcgctg gtcaccgggg ccacgggatt tgtcggctca
     2461 cacctggtgc gcgagctgct gagccggggc tatgcggtgc ggatcctcgc ccgcaacgcc
     2521 gggcgtgcgg cttccctggc caaggctggg gcggaggttc gtctcggcga tctcggtcag
     2581 cccgagacgc tccatggaat tgccgagggg atggatgtgg tgttccatct gggctcggcc
     2641 atgcgcggca actcggaagc cttcgagcgg gtggacctgg gcggtaccca cgccttgctc
     2701 gccgaagcgc agcgggccgg agtgcggcga gtcgttttcg ccgggaccct cgcctgctat
     2761 ccgctcggtc agcaacgcga tggtgcagtc atcaacgagc agtgcccctt cgatgagacc
     2821 ggcctgctcg gaaactatgc ccgcgccaag gttcgggcgg aaacggccgt gctggaggcg
     2881 gcgcgctcgg gaaagatgga gggcgtcatc gtgcggctcg ggctcgtctg tggcgagggg
     2941 gccagtattt ttcccgcgca cgtctgccag aagattgcgc gcaacctggt ggtcctgtac
     3001 ggagatggtc gtgtgccgtt gcctctgaca ttcatcgata acgccgtgca cgctttggtc
     3061 ctcggcgcgg aggtgccggg tgtcagcggc gagtctttca acatcgtcga tgacgatgtc
     3121 atgacccaac agcaatacct ggagctgttg cgtcgctata tgggcggggc gccacgcgtg
     3181 gtgcgcatgc cgcggctcgc ctactatgcg ctgggcctca tgaccgagat cgccgctgca
     3241 ctgcgcaaga aggagcctgc cacgacccgt taccgcatcc gcaaccgcct cgtgcccgtc
     3301 cgatgggact gctccaaggc gcagcgcgtg ttgaagtggc gaccgcaggt gccgctcctg
     3361 gaagggctga gccggacctt caccgcgcat gcaggacgcc atgctggata gctccgctcc
     3421 caagctctcg gtcatcgtgc ccttctacaa tgagcaggag agcattcgac cgatgtacga
     3481 agccatcgtg gccggggtcg ccccgctcat ggttcccttc gagatggtgt tcgtggacga
     3541 tggcagcaag gacgacacgc tggtgcgcgc tcgcgccatt gcagagaacg atccgcgggt
     3601 gcgcgtggtg aagttccggc gtaactatgg tcagacggct gccatggccg ccggcatcga
     3661 atacgcctgc ggcgaggtgc tcgtcacaat ggacggggac ctgcagaacg acccctcgga
     3721 tatcggtctg ttcctgcaga agatcgacag cggctacgac atcgtggtgg gctggcgcca
     3781 ccagcgccag gacaagctcg tctcgcgcaa gatcccctcg cgcatcgcaa actggctgat
     3841 cggcaaggtc acggggatcc cgatcaagga caacggctgc tcgctgaagg cgtaccgagc
     3901 gaaggtcatc aagccgatcc cgctctactc ggacatgcac cgcttcattc cggccatggc
     3961 ttcgatcgcc ggccctcgga ttgccgaggt caaggtgaag caccatgcgc gccgctttgg
     4021 cagctccaag tacggcctgt cgcgcatcta taaggtgctg ctcgatttga tggtcatcaa
     4081 gacgctggcc tcgttcgccg cgcgccccct gctgtggttc gggctgctgt ccctgccgct
     4141 gctgctggcc ggtacggccg tgctgggcta cgccctgatc cgggcgctgc tcgatggggc
     4201 gcccccctcg ctccccatgg ccggcgcggg catcatcttt ctcacctcgg ccttcatcat
     4261 gctctgcagc ggagcgatgg gggagctgat ctacaatctc ggcgacatgc gcgatcatga
     4321 gttcgcctgt ctgacccagc agctgcgcac gcgcgcggcc aggaattgag gcgcccgccc
     4381 gatgacaaac cgcaaagtgc agctctcggt catcattccg gtcgggcgtc ggcacgccga
     4441 catcggcgag ctgtacgccg aatatcgggc cggcatcgac ccgctgcgcc tgacgtacga
     4501 gttcattttc gtgctcgatg gtccgaacgc ccaggtgctg agcgcgctcg aggcgctgat
     4561 cgatgccggc gatcacaaca tcacgctcgt gtgcctgaac cgctccttcg gcgagtccac
     4621 cgcgctcatg gccggcttcg agcgctcctc gggcgacctg atcctgacgc tccccgccta
     4681 tcaccaggtc gagggagcag gcctcgccaa gctggtgagc gggctcgact gcgccgatgt
     4741 cgtggtcggc tggcgctggc cgcgggccgg ggggatgctg gagaggctgc ggcgcaacgc
     4801 ctttcaccgc atggtttcac tcgtcaccgg catgcgcctg cacgatctgg gctgcggggc
     4861 gcgcgcgatg aagcgcggcg tgctcgaaga gctgagtctc tatggggatc gtcaccgcct
     4921 gctggcgatt cttgccgatc ggcagggctt ccgcgttcgc gaggtcgagc tcacccagtc
     4981 gaaccgcgat cgcttcgatg gaatctaccg ccccggcgat tacacccatc tggtgctgga
     5041 tgtgttcacg gtcttcttcc tggtgcgctt caccaagaag ccgctgcgct tcttcggcgc
     5101 gctcggtgcc acgatgttcg gggtgggcgc actgctggtg ctgtacctgg tgatcgaccg
     5161 gctcgctttc ggcaccagcc tggcggatcg ccccgccctg ctgctttccg cgctcgtcgt
     5221 ggtgctcgga ctgcagctgt tcgcactcgg tctgctggcc gagctgatca tcttcaccca
     5281 tgcgcggcag atcaaagact accgcaccgc cgagatcatc cagtacccct caccgccggc
     5341 gcgagccgca cgagaggctg cacccgagaa ggcggcgttg cggtaatcgc acttcgtggg
     5401 cgcccaacgg ggttatgcac gcgactggat actgtgggcc ctcgccctcc tgaccggctg
     5461 ctgcctgatc gccgggatcc tcgctcgctt cgccggtctg ggccgctggc cgttgacggt
     5521 tgacgagtac tacgtcgccc agtcggtcca gaacattctg cgggagggaa ttccccacta
     5581 cgcgtgcggc gggctctacg tgcgcggact cgccttgcag tatgtctccg ctgggctgca
     5641 gctggcgggg ttgtcggcgg agcttgcgcc gcgactcgtt gccgcactct ccagcctcct
     5701 cgcgctgccg gccgtctacc tgctcgggcg ccgggtgggc gcacgcgata tcggcctgct
     5761 ggcggtcgcg atcctggcca tttccgtgtg ggagaccgaa gtcgcgcgct tcgggcgcat
     5821 gtacgcgcct tttcaggcgg tattcgtgtg gtatctggtg tgcttcctgg cctacacgct
     5881 ggatcgaagc cgccgggctc tggtgggcat gctggtgctc tcggtgctcg gtgtgttggt
     5941 ctgggagggc ggggttttcc tcgcgctggc gaacctgctg ccgcccttca tccgcaagcc
     6001 ctcgggacgc ttcgaggcac gcgactggaa gtatctcgcc ggctgcgcgc tgctggtgct
     6061 gccgagctac tggctggcga ccgcggacct gcgcacccga ggcgccgaac cgaccctgcc
     6121 ggccgattac caggagccgg cggcgacggc ccttagccgg ctggactcag cgaacctgcc
     6181 cctgagcagc ttgcacctgc acccgatgtg ggcgctggcg gtgctattgc cggtgctgct
     6241 ggccctcttt gcggtggtca gactggcgcg cgcctatgcg ggctctctgc gcgccgcggc
     6301 gggaacggat gcgcttgccc cgccgcaggc tctcggtgcg ctcgcattgc ttggttgcag
     6361 tgcagcgctc ggctgcgcgt tgatggcgca gttcggtctg gccttcgctc tagtgctgct
     6421 catgggactg ctgtacatcc tcccgtggtc cgccctgttc cggcgcgaga tgtggccgtt
     6481 tcatgccgcc atcctgctca gccttgtgct gtggatcggt ttcggtctgt ccacccccga
     6541 ctggcacgca cccgggctca accgactgca tgtcgatttt ctgctgggct atgagttcgt
     6601 gcgtttcccg gacgtcgtgc gacagatcgc agcgccctgg gggcattccg tcccggtgtt
     6661 gagcgcggcg ctgctcgtgc tcatcggtgc cgcctgcatc cacgcctgca tgctcccggc
     6721 cgagaccggc gcggccgagc gggttctgct cgcgctgctg gtcattttcg tgctcgcagc
     6781 cggagcaagc catccgccgc gccaggagac ccgctacgtc ttcttcctct acccgctggc
     6841 gattgtgctc gccaccatgt cggtcagtcg catcgcgcac gagctgtttg gcgcgcggcg
     6901 actcgccgcc gcagccaccg tggtcctcgc gctcctcggc tttgcactga cggaggactt
     6961 ccgtccgcgg cacctgtgga acatcgatac cgaggccgtc acctttcgca tcggcatgcc
     7021 gccgcgcgaa gcggcccact accaggagcg cagcgatatc cgctcggccg cccgctggct
     7081 ggcggcccat gcgagccctg gccgggatat cgtcatcaac agctttccag gcgtcgattt
     7141 ctactacccg cacgctgact tctattacat ggaagagagc gatccacgct tcgagagttg
     7201 gtcgtgtcgg cgcgggcgct tggagcgctg gagcaacctg ccgctgctgc actcggtcgg
     7261 cgcattgcag agcgagctcg ccagcgggcg gcgcgtgtgg ctggtgctgg agacgagccg
     7321 cctgccgcag ctcgagtccg ccttcgctgc gggaacctgg cagctcgaat ggagctcacc
     7381 cgagcatgac atcgcgattc tctcgagcca cgcgcgtacc ccaacacagt gaggtcatac
     7441 gagcgcaact gtgtcgattc agcgcgcgtt cagcgaagcg tctcgcgcag cacgcacggc
     7501 gcagcatttg tcttatttgg cagacagagt ctgtgcagcg ccgtgcagag cgactgtatg
     7561 cagcgccggt atgccgccaa gtcgttcgcc tgtatactgc ttcgcgcgcg gcgcatgtcg
     7621 caaaaagctt tcctcctcgt gcacctgcgg tgatatgtca acgaggcggg agcacggaga
     7681 acaaggcaga cgcacgcatg cgaccacgct agaacgtgcg ctcgagcgca catgaccggg
     7741 gattcgccag tgtccgactt tgacgcccgc gttccgggca gacatacgag cctcctctcg
     7801 caccggggcg gtgtttccga tggccctgct cctgtcgcga tgctttcgcg gcatgagcct
     7861 tgctgaggat gcgatttatg ctgcacttgt tccgtgtcgc gcgcctgcca ctgctggccg
     7921 ccgcctgtct gctgggcgcc gggttgacca acccggcgtt cgccgagatc ccgacgaccg
     7981 attaccacat tcatccgggc gacaagattc tgatcgggct gtacgacgac ccgaagatgc
     8041 agccggtgga aatcacggtg gcgccggacg gcaaactcgc gtttccgctg atcggggtta
     8101 tctccgccgg gggcaagacg gtggatcaac tgcgggtgga gatggagacc aagctcaaga
     8161 agttcgtctc cgagccggtc gtgacggtgg cggtgacgca ggtgctgggc aacgtggctt
     8221 acgtcatcgg ccaggtcaac aagcccggct ccttcatcat gaatccaacc atcaacgtgc
     8281 tgcaggcgct cgcgctcgcc ggcgggggca acccttacgc caagatggac gggatcatca
     8341 tcattcgcag ctcctcgggc ggccagcgcg tgttgaactt ccgctacggc caggtcgcct
     8401 cgggccgcaa tctcgagcag aacgtcaacc tggaggccgg cgatgtcgtc gtcgtaccgt
     8461 agtaccgcgg ttgtagccgc cggggcctgc tcgctctcgt gctggctcgc ggctcaggcg
     8521 caggaggtgt actggcagcc ccaggtcagc ctgacaggca cgtacaacac gaacgtggag
     8581 ctctcgccgc tggcctcgga caaggtgagc tcccagggct atatcggcga tgcggcgagt
     8641 ctgatcggca tcgcgacgcc gacctcacag accacgctcc ggcccgacat ccgctacgaa
     8701 tactatccgc aggacaagtc ggtcgatcgg ctggagggct tcctggatct gaactcgcaa
     8761 ttctcctggc agcgtgaccg cttcacgatg ctcggtcgcg cggaccgcct caccgatctc
     8821 agcgcggagc agccggaggc gcagttcaac caggtcaacc cggggctgcc aaccacgccc
     8881 acgaccggcc gcgtgatcgc caatgagacc cgcaacgaga tctacctcgt gccggactac
     8941 tcccatgccc tgaccccgct gaccaacctg ggcctgtccg caacctacca gcacctcagc
     9001 tacactccgg acgacgcgtt cgatcacgtc gattacaact actaccaggg ccgcccgttc
     9061 gttgactacc agtacaacga gcgcacgctg ttcaccgtcg gtgtgttcgc ctcccacttt
     9121 tcggcgatga acatcgactc gacctccaag gactatggcg gcgatatcga gatgcgctac
     9181 aactggtcgc cgatcttccg cagcaccatg gaggtgctct accagcggac gacgctcgat
     9241 caggtcaccc cgatgccgtt ctttgcccac accaacaact ggggcgcgaa tttcgatacc
     9301 cagtacctcg gccagaccaa ccggctcaag gcgagcatcg gacgttccat cctgcccagc
     9361 agtgccggct cgctctacaa caccgatctg gtgcggatcg agtatgacca cgacttcacc
     9421 gagcgcctca ccttcaccgg tgcggcgatg tacgagaaga tcgggacgct ggcgagcaac
     9481 ttcgcgagcg acacccgcag ctattccagc agcgggatct tcctgcgctg gatgatgacg
     9541 ccggtgctgt tcatcaaggg cggctactcg tatatctggc agaagtatca gctcgatccg
     9601 aacagcgcgg cgaacaatca ggcgatgctg cagttcggct acattgcacg aggacgtgag
     9661 cgctgatgga tacgcaagag aatactggcg gcgtcaacag ctacatgggg atcctgcgcc
     9721 ggcgtcgtgt gtacgtcggc acgatcgcac cgctgatcac cttcgcggcc atctatcttg
     9781 ccttcacgct gccgcccgag tatcagtcga ccgccaccat cctgatagag ccctcggtgg
     9841 tgaacaagga cgtcgtcgcc cccacggtgg cgagctactc gaaccagcag atcgagatcg
     9901 tcgaagggcg ggttctgaag ctcgatacgc tcaaggctct ggtgaagagc tacgatccgt
     9961 atcctgagga caagaccatg gacctcacgc agaaggcaca gcgcgttctg cagtccacga
    10021 cgctcgagcg ggtcgatccg gtcacgatga agcccgatca ggacgcgaac gccttctcgc
    10081 tccattacaa caatcccgac ccggagcgcg cctcggagac cgccaagcgg ctcgcccagg
    10141 cgtttctcac ctacaaccag cgctcgcggg agcaggccgc gcaggatgcc accctgtttc
    10201 tgaagcagca ggccgaggcg gtcagccagc agatgcgcga gatcgatgag gagatcaaca
    10261 agttcaagaa cgcgcacggc gatgcactcc ccgaggacat ggcgcgtaat gaggctgcaa
    10321 tcgaccgcga tcagcatgac ctcgactccc tgcagcagga gatcctgcgg gtgcaggaaa
    10381 aggagggcct gctgagcgtc gagctgagcc agatcagccc gaacatgatc tcgcaggccg
    10441 gtgatctcac ggatgtggcc acggtccgga ccaagctcgc agaagcggag cagcgttaca
    10501 cgcccgatca tccggaggtg aagcgcctga aacgcgcgct ccaggacctc atggccgcac
    10561 aaggcacggc tacgaagggt ggcatcgtct ccaacgccaa taacccccag tacctgagca
    10621 ccgccagcga gctgcagtca gcccgcaagg agctcgactc gctgcatggg caggccgacc
    10681 gactgcgtgg cgagatcaat aagtacgagg agctcttgcg ccggactccg ggggtcgagc
    10741 gggagtattc ggacatcacg cgccggcgcc agagcgcaca gaccgcgtat gcgcagatcc
    10801 aggacaagct gcagcacgcc cagctcgccg aaagcttcga gagcgaggaa cagggcgaga
    10861 aattcgtgct gctgcgcgca ccgatcccgg caaagctgcc cgtgtatccg aaccggatcg
    10921 ggctgatcct gctcggcttt atcctgggtg gggcgcttgc ggccatcgcc gtcgcgatcg
    10981 ccgaatccgc ggactcgacg gtgcgcagtg tcagggactt tcccgaggcc gaggatgccc
    11041 cgattcttgc gagcatcccg cagatcgaca atagcagcga ccggcgcaga aatcgcttga
    11101 ggtttgcgac gtgggtggcg gcctatacgg ccgcggtact ggtcattgga gtggcggtcg
    11161 tctccgggct caagagcacg acgacgacgc ccgtggcgca gacgcaggct caataagggg
    11221 acacggccga cgcggctcga atatgacgat catcgaatca gctctcgaaa gggcgaaagc
    11281 actcaagtcc gggcggccgg cacttcacac accggcggcg ccagccgccg tgcatcgtcc
    11341 accgcgccgg gtcctcgatg gaccggctcc cagcgtgctc ttccagccgc agcgggtcgc
    11401 cctcgacccg cgcgccgctc gcgagaatcg cctgctgctc aacagctccg tgcaggagga
    11461 tcgcccggcc atcgccgcct acggcatgct gcgcacgcgc ctgctgcatc gcgcccgggc
    11521 caagggctgg acgacgattg gggtgacgag ccccgctccg caggatggca agtcgctcac
    11581 cgcgctgaac ctgagcctga gcctggcacg ggaacagaac aataccgtgg cgctcatcga
    11641 tctggacatg cgcaatccga gcatgtgccg ctgcctgggg atcgagccgc cggtggagct
    11701 cagggatttc t
//
LOCUS       DASRHN010000191         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_64819, whole genome shotgun sequence.
ACCESSION   DASRHN010000191 DASRHN010000000
VERSION     DASRHN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64819"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFB37_07465"
     CDS             <1..972
                     /locus_tag="VFB37_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZO22331.1"
                     /translation="MLTRQIEKAQRKVESHNFDIRKQLLLFDDVANDQRKVVYQQRNE
                     IMATADLSAAIAGIMEEAVGTLIDQHMPRNASPTDWNLQGLAEAIQKDFNTRVEPRAW
                     LEADPELDEAALRSRLASAVREAYDAKVTRIGLAESGIPIMRHVEKDVMLSMLDRHWR
                     EHLGAMDYLRQGIHLRGYAQKDYRFEYKREAFELFAAMLDRVKFETVTTLATIEVRTQ
                     EDIDREEEERRQRLMRALQAQHAEAQSVLSTDGAEPSAEQAALAREGSAGPAAGQPRA
                     GVLPMPPPADAAGTFVRSERKIGRNEPCPCGSGKKYKHCHGVLSGLG"
     gene            976..1383
                     /gene="mutT"
                     /locus_tag="VFB37_07470"
     CDS             976..1383
                     /gene="mutT"
                     /locus_tag="VFB37_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HZO22332.1"
                     /translation="MLTRVLVVAGALFDRGGRVLIAQRPAGKHLAGRWEFPGGKVGAG
                     ESEPQALARELQEELGVRVIRSRPFMRLAHEYSDRAVELSMWLVEEFAGEAAGLDGQS
                     LKWVAPGDLPKEDILEADRPFVLALQEIAGQRR"
     gene            1701..2099
                     /locus_tag="VFB37_07475"
     CDS             1701..2099
                     /locus_tag="VFB37_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22333.1"
                     /translation="MAAQNDLPDVKLDPAGLYREDTFTDRRAGTIRRLTPVTISGDAD
                     TTRPVLFSGQTQLLTPAGVLPLVFEIEAATMEEALRKFPEGVKGALEQAIDEAREMRR
                     EAASRIVVPEVGSGLGPAPGGGGGGKIKFP"
BASE COUNT          375 a          658 c          745 g          351 t
ORIGIN      
        1 atgctcacgc gccagatcga gaaggcgcag cgcaaagtcg aatcgcacaa ctttgatatc
       61 cgcaagcaac tgctgttgtt cgatgatgtg gccaacgatc agcgcaaggt cgtctatcag
      121 cagcgcaacg agatcatggc cacggcggat ctgtccgcgg cgatcgccgg aatcatggag
      181 gaggcggtcg gcacgctgat cgatcagcac atgccccgca atgcctcccc cacggactgg
      241 aatctacagg gtctggcgga ggccattcag aaggatttca acacgcgcgt ggagcctaga
      301 gcctggctcg aggcggatcc ggagctcgat gaggctgcgc tgcggagtcg actggcgagc
      361 gcggtacgcg aggcctacga cgccaaggtt acgcggattg gtctggctga aagcggaatc
      421 ccgatcatgc gccacgtcga aaaggacgtg atgctcagca tgctggaccg ccattggcgc
      481 gagcacctcg gagcgatgga ttacctgcgg cagggcatcc atctgcgcgg ctatgcccag
      541 aaggattatc gtttcgagta caagcgcgag gcgttcgagt tgtttgccgc gatgctcgat
      601 cgtgtgaagt tcgagaccgt gactacgctc gcaaccatcg aggtccgaac gcaggaagac
      661 attgaccgcg aagaggaaga acgccgccag cgcctcatgc gtgcgctgca ggcgcaacac
      721 gctgaagcgc agtcagtgct ctctactgat ggggctgagc cttccgccga gcaggcggcg
      781 ctggcccgcg agggctcggc gggtcccgcc gccggccagc cgcgggcggg tgtgctgccc
      841 atgccgcccc ccgccgacgc ggccggtacg ttcgtgcgca gcgagcgcaa gatcggccgc
      901 aacgagccct gtccgtgcgg ttcgggcaag aagtacaagc actgtcacgg agtgctgtcc
      961 ggtctcggat agcccgtgtt gactcgcgtg ctggtggttg ccggggcgct tttcgaccgc
     1021 gggggccggg tcctgatcgc gcagcgtccc gcgggcaaac acctcgccgg gcgatgggag
     1081 ttcccaggag gcaaggtcgg cgccggcgag tccgagccgc aggccctcgc acgcgagctc
     1141 caggaggagc tcggagtccg ggtcatccgc agccggccct tcatgcgcct ggcacacgaa
     1201 tatagcgatc gggctgtcga gctttcgatg tggctcgtcg aggagtttgc gggggaggcc
     1261 gccggactgg acgggcagag cctcaaatgg gtggctccag gggatcttcc gaaagaggac
     1321 attctcgagg cggaccggcc gttcgtgctg gccctgcagg aaatcgcggg ccagcggcgg
     1381 tgatgttggc agccggtacg gctggccgag cggcacttgg gatgagtcag cgagctcgca
     1441 cccgggtggt atttcggcag gtcagcacgt ccgaccctcc ggcggctcct cgggctggga
     1501 cgtcgcgagt tggctcctcg tacgagcagc ctgctccgac gacttagcgg ggtggcgctg
     1561 ccgacggatc aacggctggc cccgcgatca gccaacggcc tggctcagcc cgtggcccgt
     1621 ggggcttggt tcctcctcgg tgggccccca ttctggccct gtgtacaatg caactcgcag
     1681 cacgatgcga cgggattccc atggcagctc agaatgacct tccggacgtc aaactcgacc
     1741 cagccggact ctatcgcgaa gacaccttca ccgaccgaag ggcgggcacc atccgtcgcc
     1801 tcactcccgt gaccatctcg ggcgacgccg acacgacgcg ccccgttctg ttctcggggc
     1861 agacgcagct cctgacgcct gccggtgtgc tgccgctcgt attcgagatc gaggctgcga
     1921 cgatggaaga ggccctgcgc aagtttcccg agggcgtcaa gggtgcgctc gagcaggcga
     1981 tcgacgaagc tcgcgagatg cggcgcgagg ctgcatcgag aatcgtcgtg ccggaggtag
     2041 gttcgggctt gggacccgct ccaggcggcg ggggcggcgg gaagatcaag tttccttgag
     2101 gagagggggg tgctcgggca aacgcatcg
//
LOCUS       DASRHN010000192         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1881043, whole genome shotgun sequence.
ACCESSION   DASRHN010000192 DASRHN010000000
VERSION     DASRHN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881043"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2597)
                     /gene="leuS"
                     /locus_tag="VFB37_07480"
     CDS             complement(<1..2597)
                     /gene="leuS"
                     /locus_tag="VFB37_07480"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZO22334.1"
                     /translation="MDESYHPAAVEAAAQQHWQALEAYRAIEHDPRFPKGKFYVCSML
                     PYPSGKLHMGHMRNYTLNDVVYRFMRSSGYNVMTPMGWDAFGLPAENAAIEKKVAPAA
                     WTRANIADMKAQFAPMGFAFDWSREVTTCDPQYYHWNQWLFLRMLESGIAYRRTQVVN
                     WDPVDHTVLANEQVVDGRGWRSGAIIEKREIPGYYLAITRYTEELLEHVKHTLEGWPP
                     QVRTMQENWIGRSEGCEISFPYAPDTRSVMQSDGALKVFTTRADTVFGATFMAIAAEH
                     PIALAAASTDPRLAAFIEECRRGAVMEADLATQDKRGVGTGLHALHPFTGQPLEIWVA
                     NYVLMGYGEGAVMGVPAHDERDFEFAHRNGLPILCVVKSSSGAYEDVRKPWKAAYSEY
                     GITVNSGEFSGLEYSAAVEAIAAALHSKGLGRKRVQYRLRDWGISRQRYWGTPIPIIH
                     CEACGVVPVPEKDLPVVLPEDLLPDGTGNPLNKDERFLVCTCPQCGRAARRETDTMDT
                     FVDSAWYYMRYCCPGASTMVDERTAYWMPMDQYIGGIEHAVLHLLYSRFWTRVMRDLG
                     LVRFEEPFRRLFTQGMLLAHCYYREEASGRKRWFYPAEVEQKFDERGIPVSATAVEDG
                     KPVMYGGIEKMSKSKNNVVEPGEFIKRFGADTARAFVMFAGPPDQSAPWSDASAEGVH
                     RFLRRLWAFCHECRTVISGAKRTPLTDSSQRKVRRELHVTLQQALFDYQRMQYNTVVS
                     ASMKMLNALETVKRPEAAALLHEGVGILLRVLYPIAPHISHALWQELGYTREMGDLLD
                     APMPQPEPAALHQDELQLVVQVNGKLRAHVTVGAEASEETIRTAALAQQQVQRFVEGK
                     PVRKVILVPG"
     gene            complement(2710..4062)
                     /locus_tag="VFB37_07485"
     CDS             complement(2710..4062)
                     /locus_tag="VFB37_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZO22335.1"
                     /translation="MAAPEGTGRSLPDFSPLVERYGPAVVNVEVVEKAQSSGGPQGLS
                     PNDPFYDFFRRFGIPAPDQGPRGNSPPVRGAGSGFIVSADGYILTNTHVVANAEEVTV
                     RLTDRREFQAKVIGADERTDVAVIKINANSLPTVRLGDPAKIRPGQWVLAIGSPFGFE
                     NSATAGIISATARSLPSDNYVPFIQTDVAVNPGNSGGPLFNLAGEVIGINSQIFSRTG
                     GYMGVSFAIPIDVARNVEDQLIKTGKVVRGRIGVTIQDVNAQLAESFGLDRPRGALVS
                     SVEKDGPAAQAGLQPGDVILSVNGKGIERFGELSGAIAAMKPGSEASLEVWRGGKKQA
                     IAVKVVELKEQQQTARAGGRQNGHATDQASQFGLTVRPLDPQEKEQADTQGNLVVEQV
                     TGPAASAGVQPGDIILGVNGKRVKTVKELQEEARSSGKNVALLIQREDAQIFVPLRIP
                     "
     gene            complement(4332..>4603)
                     /locus_tag="VFB37_07490"
     CDS             complement(4332..>4603)
                     /locus_tag="VFB37_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22336.1"
                     /translation="AANDGVSGVIGPYGEVVARAPEFRADDLVSSVTPRTGLPPYAHV
                     GNWLVISLACAAILCGLWRRKRPSSGSQAPPTGTPSAAPRVNQSG"
BASE COUNT          766 a         1534 c         1486 g          817 t
ORIGIN      
        1 cccggaacga ggatgacctt tcgcaccggc ttgccctcga cgaaccgctg cacctgctgc
       61 tgcgcgaggg ctgctgtgcg gatggtctcc tcgctcgcct ccgcacccac ggtcacgtga
      121 gcgcgcagct tgccattgac ctgcaccacc agctgcagct catcctgatg cagtgcggcc
      181 ggttccggct gcggcattgg cgcatccagc aggtcgccca tctcgcgcgt gtagccgagt
      241 tcctgccaca aggcgtggct gatgtgcggc gcgatcggat agagcacccg cagcaggatt
      301 cccactccct cgtggagcag tgcggccgcc tcgggccgct tcaccgtctc gagcgcattg
      361 agcatcttca tgcttgccga gacgaccgtg ttgtactgca tcctctgata gtcgaacagc
      421 gcctgctgca gagtgacgtg cagctcgcgg cgcactttcc gctggctgct atcggtgagc
      481 ggtgtgcgct ttgcgcccga gatcactgtg cggcactcgt ggcaaaaggc ccagagccgg
      541 cgcagaaacc gatgcacgcc ttccgcgctc gcatccgacc acggtgcgct ctgatccggg
      601 ggaccggcga acatcacgaa ggcgcgggcc gtgtcggctc cgaagcgttt gatgaactcg
      661 cccggctcga cgacgttgtt cttgctcttg gacatctttt cgatgccccc gtacatcacc
      721 ggcttgccgt cctcgaccgc tgtggcgctc accgggatgc cccgctcgtc gaacttctgc
      781 tcgacctccg cgggatagaa ccagcgcttg cgcccgctcg cctcctcccg atagtagcaa
      841 tgagccagca acatgccctg ggtgaacagc cggcggaacg gctcctcgaa ccgaaccaac
      901 ccgaggtcac gcatgacccg ggtccagaag cgcgaataca gcagatgcag caccgcgtgc
      961 tcgatgccgc cgatgtactg atccatcggc atccagtaag cggtacgctc atcgaccatc
     1021 gtgctggcgc cggggcagca ataacgcatg taataccagg cggagtccac gaaggtatcc
     1081 atcgtgtcgg tttcgcgacg ggccgcccgg ccacattgcg gacaggtgca gacgaggaag
     1141 cgctcgtctt tgttgagcgg gttgccggtg ccgtccggca ggagatcctc gggcagcacg
     1201 accggcagat ccttctccgg cacgggcacg acgccgcagg cctcacagtg aatgatgggg
     1261 atcggtgtgc cccaatagcg ctggcgcgag atcccccagt cgcgcagccg gtactgcacg
     1321 cgcttgcggc cgagtccttt gctgtgcagc gccgcggcaa tggcttcgac cgcggcggag
     1381 tactcgaggc cggagaattc gcccgagttg acggtgattc cgtattcgct gtaggcggcc
     1441 ttccagggct tgcgcacgtc ctcgtaggcg ccgctgctgg atttcaccac acagaggatc
     1501 ggcagcccat tgcggtgggc gaactcgaag tcacgctcgt catgggcggg cacacccatc
     1561 accgcgccct cgccgtagcc catcagcaca tagttggcga cccagatctc cagcggctgg
     1621 ccggtgaagg gatgcaatgc atgcagtccc gtaccgacgc cgcgcttgtc ctgcgtggcg
     1681 agatccgctt ccatcacggc gccgcggcgg cactcctcga tgaacgcagc aagccgcggg
     1741 tcggtgctcg ccgcggccag ggcgatagga tgctccgcgg cgatcgccat gaacgtcgcg
     1801 ccaaacaccg tatcagcgcg cgtggtgaat actttgagcg ccccgtcgga ttgcatgacc
     1861 gagcgggtgt cgggcgcgta tgggaacgag atctcgcagc cctcgctgcg gccgatccag
     1921 ttctcctgca tggtccgcac ctggggcggc cagccctcga gcgtatgctt cacatgctcg
     1981 agcagctcct cggtatagcg ggtgatcgcg aggtagtagc ccggaatctc gcgcttctcg
     2041 atgatcgcac cggagcgcca gccccgccca tcgaccacct gctcgttcgc cagcacggtg
     2101 tgatcgacag ggtcccagtt gacgacttgt gtcctgcggt aagcgatgcc gctttcgagc
     2161 atccgcagaa agagccactg attccagtgg tagtactgcg gatcgcaggt cgtcacctcg
     2221 cgtgaccagt cgaaggcaaa gcccatcggc gcgaactgcg ccttcatgtc ggcgatgttg
     2281 gcccgggtcc acgcggcagg ggcgactttc ttctcgatcg ccgcgttctc ggctggcagg
     2341 ccgaaggcat cccagcccat gggggtcatg acgttgtagc cgctcgagcg catgaagcgg
     2401 taaaccacat cattcaacgt gtagttgcgc atgtgtccca tgtgcagctt gccgctcggg
     2461 tacggcagca tcgagcagac gtagaacttc cccttgggaa agcggggatc gtgctcgatc
     2521 gcccgatagg cctcgagcgc ctgccaatgt tgttgcgcgg cggcttccac ggccgccgga
     2581 tgatagcttt cgtccatcga aaaaggcttt agtctcaggc gcttgcgtca cccgagagca
     2641 tacacgactc gtgaccgaaa tcctgcagcg cggcgcgcgg cgccggccag cgcgccgccg
     2701 ctgcggatcc tacggaatgc gcagcggcac gaagatctgc gcatcctccc gctggatgag
     2761 cagcgccaca ttcttgccgg aggagcgcgc ctcttcctgc agctccttga cggtcttcac
     2821 acgcttgccg ttcacgccga ggatgatatc gcctggctgc acgccagcgg aggccgcggg
     2881 cccggtgacc tgctcgacca cgagattgcc ctgcgtgtcg gcctgctcct tttcctgcgg
     2941 atcgagcggc cgcacggtca gtccgaactg actggcctga tcggtcgcat ggccgttttg
     3001 cctcccgcca gcgcgcgcgg tctgctgctg ctccttcagc tcgacgacct tgactgcaat
     3061 cgcctgcttc ttgccgccgc gccagacctc caggctcgcc tcgctgcccg gtttcatggc
     3121 cgcgatggcg cctgagagct caccgaagcg ctcgatccct ttgccattca cagacaggat
     3181 cacatctccg ggctgcagac ctgcctgcgc ggcggggccg tccttttcga cagagctcac
     3241 cagcgcgcca cgcggccggt cgagtccgaa cgactcggcg agctgtgcat tgacgtcctg
     3301 gatcgtgacg ccgatgcggc cgcgcaccac cttgcccgtc ttgatcagct ggtcttccac
     3361 attgcgcgcc acatcgatcg ggatcgcaaa cgagaccccc atgtatccgc cggtgcgact
     3421 gaagatctgc gagttgattc cgatcacttc gcccgccagg ttgaacagcg gcccgccgga
     3481 gttgccgggg ttcaccgcca catcggtctg gatgaagggc acgtagttgt cactcgggag
     3541 cgagcgcgcc gtggcgctga tgatgccggc ggtcgcgctg ttctcgaagc cgaacggcga
     3601 gccgatcgca agaacccact gaccgggacg gatcttggcg ggatcgccca gtctcaccgt
     3661 tggcaggctg ttggcgttga tcttgatcac cgcgacatcg gtgcgctcgt cggcgccgat
     3721 caccttggcc tggaactcac gtcggtccgt gagacggacg gttacttcct ccgcattcgc
     3781 cacaacgtga gtgttggtca ggatgtagcc atccgcgcta acgatgaagc ccgaccccgc
     3841 gccgcgcacg ggaggcgagt tgccgcgcgg cccctgatcg ggcgccggga taccgaagcg
     3901 ccggaaaaaa tcatagaacg gatcgttcgg cgacagccct tgaggcccgc cggaggactg
     3961 cgccttctcg accacttcga cgttcaccac ggccggtccg tagcgctcca ccaggggcga
     4021 gaagtccggc agggaacgcc cggttccctc gggcgcggcc atcggcgccg cctgggcttg
     4081 tggagcaggc tgagcctggc cgcggggcag ttgagcgttg gactcccgcg agcaggccgc
     4141 gagcaggatc gaggcgcacc acaggggcca tatgctcggt ctcagggccg ggattgcggc
     4201 cgaccgtgac ttgggcgcga tggtgggccg gatcagaggc agggcccgac gggcagcaat
     4261 gggactcggg acactcatgc gcaactccgg gttatgaggg gcgaagggta ggcgtggcga
     4321 tagactcgag ctcatccgga ctggttcact cgaggtgcag cacttggcgt gcccgtgggc
     4381 ggggcctgcg agccggatga gggccgtttg cggcgccaga ggccgcagag gatggctgcg
     4441 cacgccaggc tgatcaccag ccagttccca acatgggcat agggcggcag ccccgttcga
     4501 ggcgtcacgg acgagaccag gtcatcggca cggaattcgg gagcgcgggc aaccacttcc
     4561 ccatacggcc cgatgacgcc ggagacgccg tcgttggcgg cgc
//
LOCUS       DASRHN010000193        23651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1387633, whole genome shotgun sequence.
ACCESSION   DASRHN010000193 DASRHN010000000
VERSION     DASRHN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387633"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFB37_07495"
     CDS             complement(<1..636)
                     /locus_tag="VFB37_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZO22337.1"
                     /translation="MTDSFKARTTLKTANQSYEIWSLAALPREKVARLPYSLKILLEN
                     LLRFEDGVNVTRADIEALLAWNPAAVPSHEIAFTPARVILQDFTGVPCVVDLAAMRDA
                     IVRLGGDAERVNPLAPAELVIDHSVQVDEYGKPGSLAANTAIEFARNGERYAFLRWGQ
                     TAFRNFQVVPPNTGIVHQVNLEYLGRVIFDAQVNGVARAYPDTLVGTDSHTT"
     gene            complement(758..1870)
                     /gene="mnmA"
                     /locus_tag="VFB37_07500"
     CDS             complement(758..1870)
                     /gene="mnmA"
                     /locus_tag="VFB37_07500"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZO22338.1"
                     /translation="MSEKSAEPVIVGLSGGVDSAVAALLLRRQGWAVQGLFMSNWEDD
                     DGYCTQAQDFQDARAIARELDIPLHRVSFAAQYRKRVFDQFLQEHRAGRTPNPDVLCN
                     REIKFGIALEYVRRLGGIRFATGHYARLVHGPRGLELRKACDLSKDQSYFLHAVDMSQ
                     LAQALMPLGELTKSAVRELARRAGLPVFDKPDSTGICFIGERPFREFLGRFLQPHPGP
                     IESPEGHALGTHQGLAFYTLGQREGLHIGGQRGHAEQPWYVAAKDSARNALIVVQGHD
                     HPLLASAALRTGPVHWLVGARQGSFRCTVKVRYRQPDQAASFEALPDGTARIAFDEPQ
                     RAVTPGQYAVAYDDELCLGGAVIEAVLPASRQQAAA"
     gene            complement(1870..2355)
                     /locus_tag="VFB37_07505"
     CDS             complement(1870..2355)
                     /locus_tag="VFB37_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZO22339.1"
                     /translation="MAPRKPDITVAAVTENDGHFLVVEERINRRLVFNQPAGHVEQRE
                     SLLTAVVREVREETAWRFQPEALIGVYLWRNPSSGRSTMRFAFGGTVIEHDANQRLDR
                     GIVGTHWLTRTQLQERETRLRSPLVLRCIEDYLLGQRRPLAPVAQLDLSSAGSVEAVR
                     I"
     gene            complement(2415..3020)
                     /locus_tag="VFB37_07510"
     CDS             complement(2415..3020)
                     /locus_tag="VFB37_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22340.1"
                     /translation="MPPLQMIGEIDVNGSLAQLANARYVPESLGEPAPGTTVRQGAET
                     LAVLTTLAEVRAAVKEIAAGARRLISIYTPDLEPELYDQSGFLEVIKHFVLTHSFAKV
                     RVLLVEPTRIMRDSNRFVAMGRRLSSCIDIRYVAAHAPQRASAYLIADDRAIMYRMRA
                     DTWDGIADVNNPPVARLYLQEFDGIWNASAMDHGLRVARRG"
     gene            complement(3250..4632)
                     /gene="purB"
                     /locus_tag="VFB37_07515"
     CDS             complement(3250..4632)
                     /gene="purB"
                     /locus_tag="VFB37_07515"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZO22341.1"
                     /translation="MTASISDTLRPVDALSPIDGRYRAATAPLRKLLSEAGLIRERIR
                     VEARWLLHLAKSVPQLAGSTLSPGAHSRAERLAHEPDADCAEAVKSIEARINHDVKAV
                     EYYVRQQLALAGASEASLELVHFGCTSEDINNLSYARLLQAARALLLETLETRVGELA
                     RFAASHAETAMLARTHGQPASPTTLGKEMANFAARLRRMRERWESVAILGKWNGAVGN
                     FNAHVAAVPGSDWPSISRQFVESFGLEYNPYTTQIEPHDWIAEYCDALAAVNIVLIDL
                     SRDVWGYISLGYLRQRAVVGEVGSSTMPHKVNPIDFENAEGNLGIANALLRHFSEKLP
                     ISRWQRDLTDSTVLRNVGVALGHTLIAWRALGRGLAKIAADPVSIGADVEGAWEVLAE
                     AIQTVLRAMGAPDGYERLKEFTRGRTIDAESLAAFIDTLPLDDEARARLKALRPEGYT
                     GIAARLAREL"
     gene            4763..6166
                     /locus_tag="VFB37_07520"
     CDS             4763..6166
                     /locus_tag="VFB37_07520"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZO22342.1"
                     /translation="MTTSFRIERDSMGELQVPADALWGAQTQRAVQNFPISGLPMPRA
                     FIAALGLLKQAAARANTRLELLEPAVAQAIDAAAAEVAAGRHDAQFPIDVFQTGSGTS
                     TNMNANEVIATLATRKLGRPVHPNDHVNMGQSSNDSIPTTIHVSAALEVRRELVPALE
                     HLRDVLRQKEREVGAIVKTGRTHLMDAMPVTFGQELSGWRTQIEQGLERLASVEPRLL
                     ALAQGGTAVGTGINAHPQFGLRFTQELSDLTGLPFSSGRNFFEGLSCQDTAVELSGQL
                     KVIAVGLMKISNDLRWMNSGPLAGLGEIALPALQPGSSIMPGKVNPVIPEAVTMVAAQ
                     VMGNDLTITIAGQSGNFQLNVMLPVIAHNLLESIRLLANASRALADNAIAGFKVNQTR
                     IAEALDRNPILVTALNPVIGYEKGAAIAKKAYAEGRPIREIAEKMTDLKRDELARLLD
                     PADLAQGGIKGGSGGGG"
     gene            6166..6885
                     /locus_tag="VFB37_07525"
     CDS             6166..6885
                     /locus_tag="VFB37_07525"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZO22343.1"
                     /translation="MVQGSGAEQDGIRGRLATPDWRSCIEAKLANTQPRHAPQDWLIP
                     GLSAEETRAYRDFFPPAPVPAAVLIPLIERAEGLTVLLTQRASQLTRHAGQISFPGGH
                     IEASDRGPLAAALREAREEIGLDERLVSVAGYLPDHILLSGFRVTPVVGFVQPGFELL
                     LDEREVEDTFEVPLSHVFEPLNHRARKRRFGRDADVEVWDIRYGERNIWGATAGMLLN
                     LYKLCAPGAATASATGGPADE"
     gene            6878..7702
                     /gene="mazG"
                     /locus_tag="VFB37_07530"
     CDS             6878..7702
                     /gene="mazG"
                     /locus_tag="VFB37_07530"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZO22344.1"
                     /translation="MSEAARAIEALLQLMARLRDPATGCPWDREQSFATIAPYTLEEA
                     YEVADAIDRGDPAQLRDELGDLLFQIVFHARLAEERGWFAFAQVAESIHAKLVRRHPH
                     VFAGTAVRDSQQQSANWEELKAAERAERAQAQQAPDTSALAGIPRALPALARAAKLGR
                     RAARVGFDWQEPAQVRDKVLEELREVDEALAPSAEPADAVRAQRIAEELGDLLFAIAN
                     WTLHLKVDPEEALRAANTKFEQRFRLMEGMARERGSPLESLDAAAWDELWRRAKSS"
     gene            7861..8157
                     /locus_tag="VFB37_07535"
     CDS             7861..8157
                     /locus_tag="VFB37_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZO22345.1"
                     /translation="MTDPRPASCWPKLAMVLLAALPAGLCAAPAAPVQPIRLAAKAIS
                     MDQAVKMAEQRFKARVVRAESQQSEGHTVYVLRLLNDSGRVWTVRVDASNGNVM"
     gene            8169..8855
                     /locus_tag="VFB37_07540"
     CDS             8169..8855
                     /locus_tag="VFB37_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO22346.1"
                     /translation="MRVLVVEDESSLRESLKTRLAEAGFTVDVASDGEEGLFAGVEFP
                     LDVAVVDLGLPKLPGLELIRRLRAQRKSYPILILTARDRWQDKVEGLQAGADDYVSKP
                     FHFEEVLARVQALLRRAGGWASPELVCGEIALDTRAQTVKVSGAPVELTSFEYRILEH
                     LMLRAGEVISKTELTERLYDEESERDSNVIEVLVGRLRRKLDPQDTLRPIETLRGRGY
                     RFALARASTA"
     gene            8866..10209
                     /locus_tag="VFB37_07545"
     CDS             8866..10209
                     /locus_tag="VFB37_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22347.1"
                     /translation="MHSLSRRLLVSVSLPLALFFGVMMVVLDTGFRALSEHSLQELLG
                     AQMVALIAAAEPQAGGGYAPPPGDLASRLRTPRSGLYAQIRSAGGRSVWRSPSTVGAP
                     IDFGPALDPGARRFAYGTSDHERIAIESRGIQFEDSEGNWELTFSVAASLTPYEQQLW
                     RFRRELFGWFIGLMLLLLITLAGLLRWVLAPVRRMEREIHEVEEGRSEGLGGGYPREL
                     SGVATNLNTLLIGERKRVARYRDTLGNLAHSLKTPLAVMRAALSGNNPESVLGPEIDR
                     MSGIIEHQLKRAAASGGALLGQAPVEVAPIASELRVALLKVYARKDLSIELAVPAGVH
                     FIGDRGDLTELLGNLLDNGCKWCRSLVRLTIEAQGSFAARERLHIVVEDDGPGISAEN
                     RARIGQRGVRTDENVPGHGIGLAMVQDTVEMYSGTLIIDSSPLGGARFSLRLPGR"
     gene            complement(10206..11744)
                     /locus_tag="VFB37_07550"
     CDS             complement(10206..11744)
                     /locus_tag="VFB37_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO22348.1"
                     /translation="MSSHDLHSLLSKLGLSGTNPGAWSGSHGWSEARDNALINVRNPA
                     DGSLIAQVRPANAEDYEHVLTSATEAARLWRRVPAPKRGEAVRLLGEELRRHKNELGT
                     LVSLENGKILAEGVGEVQEMIDIADFAVGQSRMLYGLTMHSERPQHRMYEQWHPLGVV
                     GIITAFNFPVAVWSWNACLAAICGNISVWKPSPKTPLTALAVQHLCNRTMEAHGLPPI
                     FQLFIDSGTQLATRFVDDPRVGLVSFTGSTAVGRKVGERVAARLGRTLLELGGNNAVI
                     VDETANLDLAVPAIVFGAVGTAGQRCTSTRRILVHSSRLEELEKRLVHAYGQVRIVNP
                     LEAQTLMGPLIDAAAVERYQASIASARAAGGEVLYGGKVLSRPGNFVEPTIIRAHNEW
                     PIVQTETFAPILYLIPVDSLEEAIGVQNASAHGLSSALFTDRLQHAEDFLSASGSDCG
                     VANINLGTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTNTINWSRELPLAQGIE
                     FKLD"
     gene            complement(11741..12916)
                     /locus_tag="VFB37_07555"
     CDS             complement(11741..12916)
                     /locus_tag="VFB37_07555"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZO22349.1"
                     /translation="MHRVLILGAGKIGALISGLLAESGSYKVQLGDVDGAAAEAVVRA
                     HAAANLSAVRLDASDGPSLARELAERPVDAVISSLPFYCNVAVAEAARRAGTHYFDLT
                     EDVAVTRGVRAIATGASQAFVPQCGLAPGFISIAAAELITHFDELRAVKLRVGALPQH
                     PNNVLKYSLTWSTEGLINEYGNPCQAIVDGQVLDVAPLEGLEEIEIDGTLYEAFNTSG
                     GLGSLAETHGVHCESMNYKTIRYPGHCEQMRLLMNDLKLNQDRGTLKRILENAVPQTL
                     QDVVIVYAAVTGKQDGELREENYVNKVYPQLIAGRLWSAIQVTTAAGITSVVDLVLEH
                     RGKYSGFIAQEQFRLPEILANRFGRYFAPGGTKDLSSQVVVSGKAGNQRKLHKVASR"
     gene            13071..14450
                     /locus_tag="VFB37_07560"
     CDS             13071..14450
                     /locus_tag="VFB37_07560"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HZO22350.1"
                     /translation="MRSPEGTSWHSASWQSRQAQQQPAYADSQALERAVAQLSRLPPI
                     VVSWEVDALRERLAAAQRGQAFLLQGGDCAETFADCESDQIAKKLKILLQMSMVLLHG
                     LKKPIVRVGRMAGQYAKPRSADTETRESLTLPAYRGDLVNRPEFTPEARAPDPELLLR
                     GYERAALTLNFVRALVDGGFADLHHPEYWDLGFVKHSPLKDAYQRIVRSIGDALDFFE
                     GMSGHAVHEARLVSFYSSHEGLHLLYEQAQTRFIPRQNRWYNLSTHFPWIGMRTAQLD
                     GAHVEYFRGISNPIAVKVGTAMDAAWLQGLVTTLNPQNQPGRLTLIHRFGARDIEAAL
                     PRAIAAVRETGHSVLWICDPMHGNTEISTGGLKTRRFENILRELELAARIHAEMGSYL
                     GGVHIELTGDDVTECTGGARGLTDSDLQRAYRSQVDPRLNHEQALELAMLIAERSQSR
                     RAEWRNPAS"
     gene            14463..15791
                     /gene="icd"
                     /locus_tag="VFB37_07565"
     CDS             14463..15791
                     /gene="icd"
                     /locus_tag="VFB37_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZO22351.1"
                     /translation="MQYEHIVVPTGGHRISVNTDSSLNVPDRPIIPFIEGDGIGIDVT
                     PAMRRVVNAAVQKAYGERRAIEWMEIYSGEKANSRYGQWFPDETLQALRAFVVSIKGP
                     LGTPIGGGIRSLNVAMRQSLDLYACVRPIRHFPGVSSPMQDASLTDMVVFRENTEDIY
                     AGIEWPAGSAEVHKLISYLQREFGVTGIRFPASSGIGIKPVSKEGSQRLIRKAIQYAI
                     DNDRVSVTLVHKGNIMKFTEGAFRNWGYQLAREEFGAREIGGGPWLAFPNPTTGTDIV
                     IKDVIADNFLQQVLLRPEEYDVIATLNLNGDYVSDALAAQVGGIGIAPGGNIGEGLAV
                     FEATHGTAPGFAGKDRVNPGSLILSAEMMLRYLKWSEAADLIIKGMTNTIASKELTYD
                     LARLRQAIRTPKRELAARRNVEKDLQRLIPGATLMSCSGFASAVIRHMDD"
     gene            15784..16461
                     /locus_tag="VFB37_07570"
     CDS             15784..16461
                     /locus_tag="VFB37_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZO22352.1"
                     /translation="MTEGPRRTQSYDPACTLCPRLAAFLDATRARHPGYWARPVPSFG
                     AARPRLLIVGLAPGMHGANRTGRPFTGDHAGILLYETLHELGLSTGPVSVAADDGLEL
                     RNARIANAVKCVPPENKPLPEEIRRCNAYLQAELASLRSVRVVLALGRIAHDSVLLAQ
                     GLKRSAFPFAHGQEHSLADGRYLIDSYHCSRYNTQTRVLTAPMFKQVVARACTLAELS
                     GPTSRAR"
     gene            16458..18281
                     /gene="uvrC"
                     /locus_tag="VFB37_07575"
     CDS             16458..18281
                     /gene="uvrC"
                     /locus_tag="VFB37_07575"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZO22353.1"
                     /translation="MTDTTFDAKEFVGSLPRRPGVYRMYNAAHEILYIGKARNLRDRV
                     GTYFAASNVNPKIQALVQQIAAIEVTVSNSETEALLLEYNLIKAHKPRFNVVLRDDKS
                     FPYIQVSTDHEFPRLAFYRGPRSAPGRYMGPFPSAGAVKDTLRELQKLFRIRNCRDSF
                     FANRSRPCLQHQIGRCSAPCVGLITREAYAQDIDAAVKVLEGRDAEVTAQLQQRMEAA
                     AAQLDFERAAQIRDQLAALKRILAQQIVTDDGERDVDVFAIAGEPGEYAVSVMLVRGG
                     RNLGTSSYFPRGALAEPEEALSSFVMQYYATQEPPPQVLLGRKLEDADPLSQALGGRV
                     GRRVEVRCPVRGLGVRYVELARENADQALRMKLAQRQGIQEMLADLARELDLPEVPGR
                     IECFDISHTGGEGTVASCVVFGPEGALKKEYRRFNISGVAPGDDYGALRQALERRYVR
                     IRQGEVPLPDLLLIDGGQGQIALVHEVLEGLGFGDLALVGVAKGPDRRPGQERLFIHG
                     VPEPRIPEAHSPASRLVQRIRDEAHRFAITGHRRKRARRYNESVLEAIPGLGPAKRRA
                     LLKHFGGMQGVLRAGTADFEKVTGIGAALARSLYDHLHPGQ"
     gene            18311..18898
                     /gene="pgsA"
                     /locus_tag="VFB37_07580"
     CDS             18311..18898
                     /gene="pgsA"
                     /locus_tag="VFB37_07580"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZO22354.1"
                     /translation="MSTHTLPAKPAVRWNWPNTLTWLRIAAIPLIVILFYMPYPWADP
                     SAGLLFAAAGITDSLDGYLARRLGQTSRLGAFLDPVADKLIVAVALVLLVSKDHRALI
                     VLTAVVIIGREITISALREWMAEIGERRKVAVSQLGKYKTILQIVGLSFMLFRRELWG
                     LPIYRIGVVLTELAAAATLYSMVAYLRAAWPELKR"
     gene            19048..19123
                     /locus_tag="VFB37_07585"
     tRNA            19048..19123
                     /locus_tag="VFB37_07585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19081..19083,aa:Gly,seq:gcc)
     gene            complement(19192..19806)
                     /locus_tag="VFB37_07590"
     CDS             complement(19192..19806)
                     /locus_tag="VFB37_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZO22355.1"
                     /translation="MTTRKVPLKRAAMLTALAALLGASTLSAEEVHYTLDPMHTYPSF
                     EADHFGVSTWRGKMKHSKGTVTLDRAGHSGTVNITIDPASIDFGLDALNSWAVGPEFL
                     NTSQYPQAAYVGRFTHFNGDVPTQVDGELTLHGITRPVSLTLTSFACKPHPLLKREWC
                     GADAFATFQRDQFGLSAGKDYGFKMEVNLRIQVEGMRDEPKGAK"
     gene            20385..20455
                     /locus_tag="VFB37_07595"
     tRNA            20385..20455
                     /locus_tag="VFB37_07595"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20417..20419,aa:Cys,seq:gca)
     gene            20660..21940
                     /locus_tag="VFB37_07600"
     CDS             20660..21940
                     /locus_tag="VFB37_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZO22356.1"
                     /translation="MPQLTDTKIRNAIKEAVKTQRRRKLYDQLGLYLILVPREKGCGC
                     WWRQDFDWNDRERTLSLGVYPDIGIKAARQRRDEIRAQVANNVDPSTARREKKAAQRD
                     SSERTYEVIARQWLDLTAKSRHWTADHVERVRRRQEAHVWPWLGRRPVAEITEEDVLS
                     CLKRVTDKNLIDTAHRLRADTDGIFRYAKKWKFIKSNPMADLRDRDTLPKLKTTHNAA
                     ITNPSALGTLLRAIDGYPGSFIVKCALKLQALVFVRPGELRLATWDEFDLDGAEWRIP
                     AKRMKMRIEHVVPLARQAVAILRDLYPLTGPDGFVFPQVRSPFRGISNNTLGAALRTL
                     GFSADQQSAHGFRRTASTLLNESGKWHPDAIERQLAHKEPNETREAYNAAQHLPKRRK
                     MMQAWADQLDALRKGRAASGDGAAIEGSANPAAL"
     gene            22049..22570
                     /locus_tag="VFB37_07605"
     CDS             22049..22570
                     /locus_tag="VFB37_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22357.1"
                     /translation="MNDDGKVVDPNVRLLTAGEREEIAQHWQQSADKSPRYQNGREVA
                     PPRPMTAEQLQVICDHFWRQMPAPSSVDPFSLRVVAKHQKNRSVRPVLVARMERLGHL
                     EGKRQRERAKAEQAAIDHYRAWETRMAHLLAGKDARERVKRYKQSDAFSRAPTRQKRR
                     ITPLLKSGKLNLQ"
     gene            22750..22956
                     /locus_tag="VFB37_07610"
     CDS             22750..22956
                     /locus_tag="VFB37_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22358.1"
                     /translation="MKQELLTPPIKLTKRASAWPEHEVEAVNAAKLAGKSDAEIRDLV
                     KILTAARRELLPSLLATIPQVAAA"
     gene            23038..23595
                     /locus_tag="VFB37_07615"
     CDS             23038..23595
                     /locus_tag="VFB37_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22359.1"
                     /translation="MQLYDELDRLVQQSELRTLARLIRGGLVPIDLDDAIPGYGAPIV
                     DQGYVPSRIIEILTGRDQPDRRPSGVCPECYSEWRRRKLRPRTVRCEHQHAAAHRRTI
                     RVRDFFGKVTEKRVWAVTQGIARAKNQKELAQHICPRCLPVGGDASLVVCRHRGTAAY
                     RKRRTWRVSLGLTQKELATLKELAW"
BASE COUNT         4210 a         7574 c         7755 g         4112 t
ORIGIN      
        1 tgtggtatgc gaatcggtgc cgacgagcgt gtccggatac gcccgggcca ctccgtttac
       61 ctgggcgtcg aagatgacgc ggcccagata ctccaggttc acctggtgca cgatcccggt
      121 gttcggcggc accacctgga agttgcggaa tgcggtctga ccccaccgca agaaggcata
      181 gcgttcgccg ttgcgcgcga actcgatggc ggtgttggcc gccagcgagc cgggcttgcc
      241 gtactcatcg acctgcacgg agtgatcgat gaccagctcg gcgggcgcga gcgggttgac
      301 gcgctcggca tcgccgccga ggcgcacgat cgcatcgcgc atcgcagcca gatccaccac
      361 gcacggcacg ccggtgaaat cctggaggat gacgcgcgcg ggcgtgaaag cgatctcgtg
      421 ggatggcacg gcggccggat tccaggccag caaggcctcg atgtcggcgc gcgtcacgtt
      481 cacaccatcc tcgaagcgca gcagattctc gaggagaatc ttcagcgaat acggcagtcg
      541 tgcgaccttc tcccggggca gcgcggccag actccagatc tcatacgatt ggttggcggt
      601 cttgagcgtc gtacgcgctt tgaagctgtc tgtcatgcat cctccgcagc ggctggccgc
      661 gcctgcgaat tatatcggat gggggccacc gaggcagatc ggcacacgcc cggctgagcg
      721 gtcgccggcg tgccccacgc gggcgcggca gaaactgcta cgcggccgct tgctggcgcg
      781 aagccggcag aactgcttcg atcaccgcgc cgcccagaca gagctcatcg tcgtacgcca
      841 cggcgtactg gcctggggtg acggcgcgtt gcggctcatc aaaggcaatg cgcgcggtgc
      901 catcgggcaa cgcctcgaac gatgcggcct gatcgggttg ccggtaacgc actttgaccg
      961 tgcagcggaa ggagccttgt ctggcgccga ccagccagtg aaccggaccg gtgcgcagcg
     1021 ctgcgctggc aagcaacggg tggtcatgcc cctgcaccac gatgagcgcg ttccgcgcac
     1081 tgtcctttgc cgccacgtac cagggttgct cggcatgacc gcgctgcccg ccgatatgca
     1141 gtccctcgcg ctgcccgagt gtgtagaagg caagtccctg atgagtgccg agcgcatggc
     1201 cctcgggcga ctcgatgggg cccggatgag gctgcagaaa gcggccgaga aactcgcgaa
     1261 agggcctctc gccgatgaag cagatcccgg tgctgtcggg cttgtcgaag acgggcagac
     1321 cggctcgcct tgcgagctcg cgcaccgcgc tcttggtcag ctcccccaga ggcatgaggg
     1381 cctgcgccag ctggctcatg tccacggcgt gcagaaaata actttgatcc ttgctcaaat
     1441 cgcaggcctt gcgaagctcg agcccgcgag gcccgtgcac caggcgcgcg tagtgccccg
     1501 ttgcaaagcg tatcccgccg agtcggcgca catactccag cgcaatccca aacttgattt
     1561 cccgattgca gagcacgtcg gggttgggag tacggcctgc ccggtgttcc tgcagaaatt
     1621 ggtcgaagac ccttttgcgg tactgcgcgg cgaagctgac acgatgcagc gggatgtcga
     1681 gctcccgggc gatcgcgcgg gcatcctgga agtcctgcgc ttgcgtgcaa tagccgtcat
     1741 cgtcttccca gttgctcatg aagaggccct ggacagccca tccctgccgc cggagtaaca
     1801 gtgcggcaac tgccgagtcc actcccccgg agagacccac gatgaccggt tcggcgctct
     1861 tctcggacat cagattcgaa cggcctcgac tgacccggcg gagctcagat ccagctgggc
     1921 gaccggagcg agaggccggc gctggccgag cagatagtct tcgatgcaac gcaggaccaa
     1981 aggactgcgc aaacgggttt cgcgttcctg cagctgcgtg cgagtcaacc agtgcgtgcc
     2041 cacgatgccg cgatcgagcc gctggttcgc atcatgctcg atgaccgtac ccccaaacgc
     2101 gaagcgcatc gtcgagcgtc cggaggaggg gttgcgccac aggtacacac cgatgagtgc
     2161 ctccggttgg aagcgccagg cggtttcttc gcgcacctcg cgaaccaccg cggtaagcaa
     2221 tgactcacgc tgctcgacgt ggcccgccgg ctggttgaac accaggcggc ggttgatgcg
     2281 ctcttccacc acgagaaaat gaccgtcgtt ctcggtgacg gcggcgacag tgatgtccgg
     2341 cttgcggggg gccatggcgg gaattatagc cagaccccgg tggcgcaatc agatggcgcc
     2401 cgggctggcg cacctcagcc gcggcgggct actcgaagac cgtggtccat tgcgctcgcg
     2461 ttccagatgc cgtcgaactc ctggagatag agtcgggcga ccggcggatt gttgacatcc
     2521 gcgatgccgt cccaggtatc cgcgcgcatg cggtacatga tggcgcggtc atcggcaatg
     2581 aggtaggcgg aggcgcgttg cggggcatgc gcggccacat agcgaatatc gatgcaactc
     2641 gagaggcggc gacccatcgc gacgaatcga ttgctgtcac gcatgatgcg cgtgggctcg
     2701 acgagcagca cccgcacctt ggcaaagctg tgggtcagga cgaaatgctt gatgacttcg
     2761 aggaatcccg actgatcgta cagctcgggc tcgagatcgg gggtgtagat cgagatcagc
     2821 cggcgagcgc ccgcggcgat ctccttcacc gctgcccgca cctcggcaag cgtcgtcaga
     2881 accgcgagcg tctccgcgcc ctgccgcacg gtggtccccg gggcgggctc gccaagggac
     2941 tcgggcacat aacgcgcgtt agcgagttgt gcgaggcttc cgttgacgtc gatttccccg
     3001 atcatttgga gaggcggcac tggcgtctat acctgcgcgg ggcaggcgac gtcgatctta
     3061 gttatcggag cctcccgcaa gttgtcgagc gggtcacgct ttgggcggtg accgggccgg
     3121 ccagaagtca aatcgagccg tagttcactg tttccagcgg gtctgcgtca ggcccctgcg
     3181 gggccagaaa cgcagccacg agctaagctg tcggtgggtc cggcctggct ctgcgggacg
     3241 acggctgctt cagagttccc gagcaaggcg cgccgcgatc ccggtatatc cctccggacg
     3301 taacgccttc agccgtgcgc gcgcttcatc gtccagcggc agggtatcga tgaatgctgc
     3361 cagtgattcg gcatcgatgg tccgtccgcg cgtgaactcc ttgagccgct cgtagccatc
     3421 gggcgcgccc atcgcacgca gcaccgtctg gatcgcctcg gccagcacct cccacgctcc
     3481 ctccacgtca gcgcctatgc ttaccgggtc ggccgcgatc ttcgcgagcc ctcggcccag
     3541 ggcgcgccac gcgataagtg tatggccgag ggcaacaccg acgttgcgca gcacggtcga
     3601 atcggtgaga tcgcgctgcc agcgcgagat gggcagcttc tccgaaaagt gtcgcagcag
     3661 cgcgttggcg atgccgaggt tgccttcggc gttttcgaaa tcgatcgggt tgaccttgtg
     3721 gggcatcgtg gatgagccca cctcgccgac caccgcgcgc tggcgcaaat accccaacga
     3781 aatatacccc cacacatccc gactgagatc gatcagcacg atgttgacgg ccgccagggc
     3841 atcgcagtat tccgcgatcc agtcgtgcgg ctcgatctgc gtggtgtagg ggttgtattc
     3901 cagtccgaag gactcgacga actgccggct gatcgagggc cagtcgctgc cggggaccgc
     3961 cgccacgtgc gcattgaaat taccaaccgc accgttccat ttgccgagta tggccaccga
     4021 ctcccagcgc tcgcgcatgc gccgcagccg ggcggcgaag ttggccatct ccttgcccag
     4081 agtcgtgggg cttgccggtt ggccatgagt cctcgccagc atcgccgtct cggcatggct
     4141 ggcggcgaaa cgggcgagct ccccaacacg cgtctcgaga gtctccagca gcagcgcacg
     4201 cgccgcttgc agcagccgcg cgtagctcag attgttgatg tcctcggaag tgcagccgaa
     4261 gtgaacgagc tcgagtgagg cctcgctcgc cccggccagt gccagttgct ggcggacgta
     4321 atactcgacc gccttgacgt catgattgat ccgcgcctcg atcgacttca ccgcttccgc
     4381 acagtccgca tccggctcgt gcgcgagccg ctcggcgcgc gagtgtgcgc ccggggacaa
     4441 ggtagagccg gcaagctgcg gcacggactt cgcgagatgc agcagccagc gcgcttccac
     4501 gcggatgcgc tcgcgaatga gcccggcttc ggagagcagt ttgcgcaagg gggcggtcgc
     4561 ggcgcgatac cggccatcga tcggcgagag cgcgtccacg ggccgcaggg tgtcgctgat
     4621 tgaagccgtc atgcgttatc ggcgcctcga agtgctacga tttgcatgat aacgcagcgc
     4681 gctgcccggc accgccagcg acgatggccg tggccgatcc ccctggcgag cttcagccca
     4741 gcgcagaacc ggagagcctt gcatgaccac ttcctttcgg atcgagcgcg acagcatggg
     4801 cgagctgcag gttccggccg atgcgttgtg gggcgcccag acccagcgcg ccgtgcagaa
     4861 cttcccgatc agcggactgc cgatgccgcg ggcgttcatc gctgcgctgg ggctgctgaa
     4921 gcaggcagcg gcccgggcca acacccgcct ggagctgctc gagcccgccg tggcccaggc
     4981 catcgatgcc gccgccgccg aggtggcggc cggccgtcac gatgcgcagt ttcccatcga
     5041 tgtctttcag accggctcgg gcaccagcac gaacatgaac gcgaacgagg tgatcgcgac
     5101 acttgctaca cgcaagctgg gtaggccggt gcatcccaac gatcacgtca acatgggaca
     5161 gagcagcaac gactccattc cgaccaccat ccacgtcagc gccgcgctcg aggtgcgacg
     5221 cgagctggtg ccggcattgg agcacctgcg cgatgtgctg cggcagaagg agagggaggt
     5281 cggcgccatc gtgaagaccg gccgtacgca cctcatggat gccatgccgg tgacttttgg
     5341 acaggagctc tccggctggc gcacccagat cgagcaggga ctcgagcggc tcgcaagcgt
     5401 cgagccccgg ctgctggcgc tcgctcaggg cggtaccgca gtcggaaccg gaatcaatgc
     5461 gcatccgcag ttcgggctgc gcttcacgca ggagctgtcg gatctcaccg gtctgccctt
     5521 cagcagcggg cgcaacttct tcgaggggct ttcctgccag gacacggcgg tggagctttc
     5581 cggacagctc aaggtgatcg ccgtgggcct catgaagatc tcgaacgatc tgcgctggat
     5641 gaacagcggt ccgctcgccg ggctgggcga gatcgcgctc ccggcgctgc agccgggcag
     5701 cagcatcatg cccggcaagg tcaaccccgt gattcccgaa gcggtcacca tggtcgccgc
     5761 tcaggtcatg ggcaacgacc tcacgatcac cattgcgggc cagtccggca atttccagct
     5821 caatgtcatg ttgccggtca tcgcccacaa cctgctcgag agcatccgct tgctggcgaa
     5881 tgcttcccgt gcactggccg acaatgcaat cgcgggcttc aaggtcaacc agacgcgcat
     5941 tgcagaagct ctggaccgca atcccatcct cgtcacggcg ctcaacccgg tcattggcta
     6001 tgagaaaggc gccgcgattg ccaagaaggc ctacgcggaa ggccgcccca tccgcgagat
     6061 cgccgaaaag atgaccgacc tgaagcgcga tgagctggcc cgattgctgg atccggcgga
     6121 tctcgcgcag ggcggcatca agggtggctc gggcggcggc ggctgatggt gcagggttcg
     6181 ggcgcggaac aggacggcat acgcggtcgg cttgccacgc ccgattggcg ctcctgcatc
     6241 gaggcgaagc tggcgaatac gcagccgcgg catgcgccgc aggactggct gatacccggc
     6301 ctctcagcgg aggaaacccg cgcgtaccgg gacttctttc ccccggcgcc cgtccccgct
     6361 gcggtgttga ttccgctgat cgagcgagcc gagggcctga ccgtgctgct cacccagcgg
     6421 gcgagccagc tcacccgaca tgcgggacag atcagctttc cggggggaca catcgaggcc
     6481 tccgatcggg gaccgctggc cgccgcgctg cgcgaagcgc gcgaggagat cgggctcgac
     6541 gagcggctcg tatcggtggc gggctacctg cccgatcaca tcctgctgag cggctttcgg
     6601 gtcacgccgg tggtgggctt cgtgcagccg gggttcgagc tgctgctgga tgagcgtgag
     6661 gtggaggaca ccttcgaggt cccgctgagc cacgtcttcg agccgttgaa tcatcgcgcc
     6721 cgcaagcggc ggtttggccg cgacgccgat gtggaggtgt gggacattcg ctatggcgag
     6781 cgcaacatct ggggcgccac ggctggaatg ctcctcaacc tgtacaaact gtgtgcgccg
     6841 ggcgctgcga cggcctctgc gaccggcggg ccggcagatg agtgaggccg cgcgcgccat
     6901 cgaggcgctg ctgcaactca tggcacggct gcgggatccg gcaacgggct gcccctggga
     6961 ccgcgagcag agcttcgcaa ccatcgcacc gtacacgctg gaggaggcct acgaagtcgc
     7021 cgatgcgatc gatcggggcg atccggcgca gcttcgcgat gagctcggtg atctgctgtt
     7081 tcaaattgtt tttcacgcgc gccttgccga ggagcgcggc tggttcgctt tcgcgcaggt
     7141 cgccgagtcg atccacgcca agctcgtgcg gcgccacccg catgtcttcg ccggtaccgc
     7201 ggtgcgtgac tcgcaacaac agagcgcgaa ctgggaggag ctcaaggcgg ctgagcgggc
     7261 cgagcgggcc caagcgcagc aggcccccga cacgagtgcc ctggccggca tcccgcgggc
     7321 tttgccggcc ctggcgcgag cagcgaagct cggccgacgc gcggcccgtg ttggattcga
     7381 ctggcaggag cctgcgcagg tgcgcgacaa ggtcctggag gagttgcgtg aggtcgatga
     7441 ggctctggcg cccagtgcag agcctgctga tgcggtgcgc gcgcagcgta tcgccgagga
     7501 gctgggcgat ctgcttttcg ccattgccaa ctggactctg catctcaaag tggacccgga
     7561 agaggcattg cgcgcggcga ataccaaatt cgagcagcgt ttccgcctca tggaagggat
     7621 ggcgcgcgag cggggctcgc cgctcgagtc cctggatgcc gccgcgtggg atgagctctg
     7681 gcgccgggcc aagagcagct gacaccggga gcgccagggc cctccatacc gcgctgcggc
     7741 gagtccgcgt tcagtccgca ttcaccggcg gagccctagc atgggttgtg aagttttctc
     7801 aaatcgccgc ccgccaaagg ttttcgggcc atgcgaatca gctagtcttg gcgcacgacg
     7861 atgactgatc cgcgtcctgc atcctgttgg ccgaagctgg cgatggtgct cctggcggcg
     7921 cttccggccg gcctgtgcgc ggcgcccgcc gcgcccgtgc agccgatccg gctcgcggcc
     7981 aaggcgatct ccatggacca ggcggtgaag atggccgagc agcgcttcaa ggcgcgcgtg
     8041 gtgcgtgcgg agtcgcaaca gagcgaaggg cataccgtgt atgtcctgcg gctgctcaac
     8101 gattccggcc gtgtctggac cgtgcgcgtc gatgcttcca acggtaacgt gatgtaggag
     8161 tccgtggcat gcgggttctg gtcgtcgagg acgagtcctc gctccgcgag tcgctcaaga
     8221 cgcgactcgc cgaggcgggc ttcacggtgg atgtggcgag cgatggcgaa gagggcctgt
     8281 tcgccggtgt tgagttccca ctggatgtcg cagtcgtcga tctcgggctg cccaagcttc
     8341 cgggcctcga gttgatccgc cgcctgcgcg cgcagcgcaa gagttacccg attctgatcc
     8401 tgactgcgcg agatcgctgg caggacaagg tcgaaggcct gcaggcgggg gctgatgact
     8461 acgtctccaa gccctttcac ttcgaggaag tgctggcgcg cgtgcaggcg ctgctgcgtc
     8521 gcgcaggggg ctgggcaagt cccgagctcg tgtgcgggga aatagccctc gacacacgcg
     8581 ctcagaccgt caaggtgagc ggcgcaccgg tcgagctgac gagcttcgag tatcggattc
     8641 tcgagcacct gatgttgcgt gcgggcgagg tgatctccaa gaccgagctc accgagcggc
     8701 tgtacgatga ggaatcggaa cgcgacagca atgtgattga agtgctggta ggccgcctca
     8761 ggcgcaagct cgatccccag gacacgctgc gcccgatcga gacgctgcgc gggcggggct
     8821 accggttcgc actcgcgcgc gcgtccaccg cctgaagcct gccgcgtgca ttccctcagc
     8881 cggcggctgc tcgtctcggt ctccctgccg ctggcgctgt tcttcggtgt gatgatggtg
     8941 gtgctcgaca ccggcttccg tgcgctctcg gagcactcgt tgcaggagct gctgggcgcc
     9001 cagatggtgg cgctgatcgc cgctgccgaa cctcaggccg ggggcggtta tgccccgccg
     9061 cctggagatc tggcctcacg cttgcgcacg ccgcgctcgg ggctgtatgc gcagattcgc
     9121 tcggcgggcg gccgctcggt ctggcgctcg ccctccacgg tcggcgcgcc catcgacttc
     9181 ggtcctgccc tcgacccggg ggcgcgccgc ttcgcctacg gcacgagtga ccatgagcgc
     9241 atcgcgatcg aaagccgcgg catccagttc gaggacagcg aaggcaactg ggagctcacc
     9301 ttcagtgtgg cggcgagcct caccccctat gagcagcagc tgtggcgctt ccggcgcgag
     9361 ctgtttggct ggttcatcgg gctgatgctg ctgctgttga tcaccctggc ggggcttttg
     9421 cgctgggtgc tggcgcccgt gcgccgcatg gagcgcgaga tccacgaggt ggaggaggga
     9481 cgcagcgagg ggctcggcgg gggctatccg cgcgagctga gcggggtcgc cacgaacctc
     9541 aatactttgc tcattggcga gcgcaagcgg gtcgctcgct atcgcgacac gctgggcaat
     9601 ctcgctcaca gcctcaagac cccgctggcc gtcatgcgtg ccgcactgag cggcaacaat
     9661 cctgaatcgg tactgggccc ggagatcgac cgcatgagcg gcatcatcga gcatcagctc
     9721 aaacgggcgg ccgcgtccgg cggtgcgctc ctcggccagg ctcccgtgga ggttgcgccg
     9781 atcgcaagcg agctgcgcgt ggcgctgttg aaggtgtatg cgcgcaagga tctctcgatc
     9841 gagctcgcag tccccgccgg agtgcacttc atcggtgatc gcggcgatct caccgagctg
     9901 ctcggtaacc tgctcgacaa cggctgcaaa tggtgccgca gcctcgtgcg cctcacgatc
     9961 gaggcgcagg gcagcttcgc cgcacgcgag cgcctgcaca tcgtggtgga ggatgatgga
    10021 cccggcatct ccgccgagaa tcgcgcacgc atcggtcagc gcggcgtccg cacggatgag
    10081 aatgtccccg gccatggcat cggactcgcc atggtgcagg acaccgtgga gatgtacagc
    10141 ggcacactga tcatcgacag ctcgccgctg ggcggcgcgc gattctcgct tcggctgccg
    10201 ggacgttagt cgagtttgaa ctcgatgccc tgcgccagcg gcagctcgcg gctccagttg
    10261 atcgtgttgg tctgacggcg catgtaagcc ttccaggcat ctgagcccgc ttcgcggccg
    10321 ccgccggttt ccttctcgcc cccgaaggca ccgccgatct ccgctcccga ggtgccgagg
    10381 ttgatgttgg cgacgccgca atcgctgccg ctggcggaca ggaaatcctc cgcatgctgc
    10441 aaccggtcgg tgaacagcgc ggacgagaga ccgtgagccg aggcgttttg cacgccgatg
    10501 gcctcttcga gcgagtccac cgggatcaga tacaggatcg gggcgaacgt ctcggtctgc
    10561 acgatgggcc actcgttgtg cgcccggatg atcgtcggct cgacgaagtt gcccggtcgt
    10621 gacaggacct tcccgccgta gagtacttcc ccgccggcgg cgcgcgcgct cgcgatggag
    10681 gcctggtagc gctccaccgc cgctgcatcg atcagcggtc ccatcagagt ctgcgcctcg
    10741 aggggattga cgatgcgcac ctgtccatag gcatgcacga ggcgtttctc gagctcctcg
    10801 agacgcgagc tgtggaccag gatccggcgg gtgctggtgc agcgctggcc tgcggtgccc
    10861 accgcgccga agacgattgc cgggacggcc agatcgaggt tggccgtctc atcgacgatc
    10921 acggcgttgt taccaccaag ctcgagcaga gtcctgccca gacgtgcggc gacccgctcg
    10981 ccaaccttgc ggcccacggc ggtcgagccg gtgaaggaga ccaggcccac gcgcgggtca
    11041 tcgacgaagc gcgtcgcgag ctgcgtaccg ctgtcgatga acaattggaa gatcggcggc
    11101 agaccgtgcg cctccatggt ccggttgcac agatgctgca cggcgagtgc ggtgagcggt
    11161 gttttcggcg agggcttcca gacggagatg ttgccgcaga tcgccgcaag acaggcattc
    11221 cacgaccata cggcgaccgg aaagttgaat gcggtaatga tgcccacgac acccagcgga
    11281 tgccactgct cgtacatgcg atgctgcggc cgctcggaat gcatggtgag gccgtagagc
    11341 atgcgcgact ggccgacggc gaaatcggcg atgtcgatca tctcctgcac ctcgcccacg
    11401 ccttcggcga gaatcttgcc gttctcgagc gagacgagcg tgccgagctc gttcttgtga
    11461 cgccggagct cctcgccgag gagccggacg gcttcaccgc gcttaggcgc aggcacccgg
    11521 cgccagagac gtgcggcttc ggttgccgat gtcaggacgt gctcgtaatc ctcggcattc
    11581 gccgggcgaa cctgtgcgat cagcgagcca tcggccggat tgcgcacgtt gatgagggcg
    11641 ttatcgcgcg cttccgacca gccgtgcgag ccggaccacg ccccggggtt ggtccccgag
    11701 agcccgagct tggaaagaag cgagtgcaga tcgtgcgagc tcatcgtgag gctaccttgt
    11761 gcagcttgcg ctgattgccg gccttgccgg agaccaccac ctggctcgag aggtccttgg
    11821 tgccgccggg tgcgaagtag cgcccgaaac ggttggcgag aatctcaggc agccgaaact
    11881 gctcctgcgc aatgaaccca ctgtacttgc cccgatgctc cagcacgaga tccaccaccg
    11941 aggtgatccc cgcggccgtg gtcacctgga tggccgacca cagacggccc gcgatcagct
    12001 gcggatacac cttgttgacg tagttttcct cgcgcagttc cccgtcctgc ttgcccgtga
    12061 cggcggcgta gacgatgacc acatcctgca gagtctgggg aacggcgttc tcgagaatcc
    12121 gcttgagcgt gccgcgatcc tgattgagct tcagatcgtt catcagcagg cgcatctgct
    12181 cgcaatggcc cgggtagcgg atggtcttgt agttcatcga ctcgcagtgc acaccgtgcg
    12241 tctcggcgag tgagccgagg ccgcccgagg tgttgaacgc ctcgtacagc gtgccatcga
    12301 tctcgatctc ttcgaggccc tcgagggggg cgacatcgag gacctgcccg tcgacgatcg
    12361 cctggcaggg attgccgtat tcgttgatga gtccttcggt ggaccaggtg agtgaatatt
    12421 tgaggacgtt gttcggatgt tggggcaggg cacccacgcg cagcttcacg gcgcgcagct
    12481 cgtcgaagtg cgtgatgagc tcggcggcag cgatgctgat gaatcccggc gccaggccgc
    12541 actggggtac gaatgcctga ctggcgcccg tggcgatcgc ccgcacgccg cgggtcaccg
    12601 cgacgtcctc cgtcagatcg aagtaatgcg ttccggcccg gcgggcggcc tctgcgacgg
    12661 ccacgttgca gtagaagggc aggctggaga tgaccgcatc gacagggcgc tcggccagct
    12721 cacgtgcgag ggagggccca tcgctcgcat cgagccgcac cgcggagagg ttcgccgcgg
    12781 catgcgcgcg caccaccgcc tcggcggcgg cgccatcgac gtccccgagc tgaaccttgt
    12841 aactaccgga ctctgcaagc aatccggaga tgagcgcgcc gatcttgccg gcgcccagaa
    12901 tgagtacacg atgcatggtc ttcccctggg agggcacccg caaaacgcat tcgccgggat
    12961 gcccaaaagg cgcgcattct gctccaaccg gccatcctcg cgccagctgc caggtccagg
    13021 ttccggccct ccgttggggc tcctgtcccg gggtgatagc atcgcgcgcc atgcgttctc
    13081 ccgaaggcac ctcctggcac agcgcgagct ggcagtcccg acaggcgcag cagcagcccg
    13141 cgtatgccga ttcccaggcg ctcgagcggg cggttgccca gctgtcgcgg ctgcccccca
    13201 tcgtggtctc ctgggaggtg gatgcactgc gcgagcgcct ggctgccgcg cagcgaggcc
    13261 aggcctttct gctgcagggc ggagactgcg cggaaacctt cgcggactgc gagtccgatc
    13321 agatcgcgaa gaagctgaag atcctgctgc agatgagcat ggtgctgctg cacgggctga
    13381 agaagccgat cgtgcgcgtc ggacgcatgg ccggtcagta tgccaagccc cgatcggcgg
    13441 acaccgagac ccgcgaaagc ctgacgctgc cagcgtatcg gggcgatctg gtgaatcgcc
    13501 ccgagttcac ccccgaggct cgcgcgccgg acccggagtt actgctgcgg ggctacgagc
    13561 gcgccgcgct gacactgaac ttcgtgcggg cgctggtcga tggcgggttc gccgacctgc
    13621 atcatccgga atactgggac ctgggcttcg tgaagcattc cccgctcaag gatgcctacc
    13681 agcgcatcgt ccgctcgatt ggcgatgcac tcgatttctt cgagggcatg agcgggcacg
    13741 ccgtgcacga ggcgcggctg gtcagctttt attccagtca cgaggggctg cacctgctgt
    13801 acgagcaggc gcagacccgg ttcattcccc gccagaaccg ctggtacaac ctctccaccc
    13861 actttccctg gatcgggatg cgcaccgcac agctcgatgg ggcgcacgtc gagtatttcc
    13921 gcggcatctc caatcccatt gccgtcaagg tgggcacggc gatggatgcc gcatggctgc
    13981 agggtctggt gacgacgctc aatccacaga atcagccagg ccgcctgacc ttgatccatc
    14041 gtttcggtgc acgggatatc gaggcggcct tgccgcgcgc cattgccgcg gtccgcgaga
    14101 ccggccacag cgtgctgtgg atctgcgatc cgatgcacgg caacacggag atcagcaccg
    14161 gcggcctgaa aacccgccgc ttcgagaata ttctccgcga gctcgagctc gcggcgcgca
    14221 ttcacgcgga aatgggctcc tacctgggcg gagtgcacat cgagctcacg ggcgatgatg
    14281 tcactgaatg caccggcggc gcccgcggcc tcaccgatag cgacctgcag cgggcgtatc
    14341 gctcgcaggt ggatccccgc ctcaatcacg agcaggcgct cgagctcgcc atgctgattg
    14401 ccgagcgcag ccagagccga cgcgccgagt ggcgcaaccc cgcttcctga gtcaggagcc
    14461 gcatgcagta cgagcacatc gtcgttccca ccgggggaca ccggatcagc gtcaacaccg
    14521 actcgagcct gaatgttccg gaccggccga tcatcccctt catcgaaggc gatgggatcg
    14581 gcatcgatgt gacgccagcg atgcgacgcg tggtgaatgc agcagtgcag aaggcttacg
    14641 gcgaacgacg tgccatcgaa tggatggaga tctacagcgg cgagaaagcc aacagccgct
    14701 atggccaatg gtttcccgat gagacgctgc aggcgctgcg ggccttcgtc gtctcgatca
    14761 aggggccgct cggtacaccg atcggcggcg gtatccgctc gctcaatgta gcgatgcggc
    14821 agagcctgga tctttacgcc tgcgtgcgtc cgatccgcca ttttcccggg gtctccagcc
    14881 cgatgcagga tgcgagcctc accgacatgg tggtcttccg cgagaacacg gaagacatct
    14941 acgccggcat cgagtggccc gccggctccg ccgaggttca caagctcatc agctacctgc
    15001 agcgcgagtt cggcgtcacc ggcatccgtt tcccggcgag ctcgggcatc ggcatcaagc
    15061 cggtgtcgaa ggaaggcagt cagcggctca tccgcaaggc gatccagtac gcgatcgaca
    15121 acgatcgtgt ctcggtaacg ctcgtgcaca agggcaacat catgaagttc acggagggtg
    15181 ccttccggaa ctggggttat cagctggcac gcgaggagtt cggcgcacgc gagatcggcg
    15241 ggggcccgtg gctcgccttt cccaacccga cgaccggcac cgacatcgtg atcaaggatg
    15301 tgattgccga caatttcctg cagcaggtgc tgctgcgccc cgaagagtac gacgtcatcg
    15361 ccaccctcaa tctcaatggc gattacgtct ccgatgcgct cgctgcacag gtgggcggca
    15421 tcggcatcgc cccgggcggc aacatcggcg aggggctggc ggtattcgag gccacccacg
    15481 gcacggcgcc gggctttgcg ggcaaagacc gggtgaaccc cggctcgctg atcctgtcgg
    15541 cggagatgat gctgcgttat ctgaaatgga gcgaggccgc ggatctgatc atcaagggca
    15601 tgaccaacac gatcgccagc aaggagctga cttacgacct ggcgcgtctg cggcaggcca
    15661 tccgcacgcc caagcgcgag ctggccgccc gcaggaacgt ggagaaagat ctccagcggc
    15721 tgatccccgg agcgacgctc atgagctgct cgggctttgc gagcgccgtg atccggcaca
    15781 tggatgactg aggggcctcg ccgaacgcag tcctacgatc cggcctgcac tctctgcccg
    15841 cggctggcgg cctttctcga tgcgacccgc gcccgccatc ccggctactg ggcccgcccc
    15901 gttccctcct ttggggccgc acgcccccgg ctcctcatcg tcggactcgc gccgggaatg
    15961 cacggtgcga atcgcacagg gcggcccttc acgggcgatc atgcgggcat tctgctgtac
    16021 gagacgctcc atgagctggg gctatcaacg gggccggtat ccgtcgcggc cgatgatggc
    16081 ctggagctgc gcaacgcgcg cattgccaac gcggtcaagt gcgtaccgcc ggagaacaag
    16141 cccctgccgg aggagattcg tcgctgcaat gcctacctgc aggctgagct tgcgtcgttg
    16201 aggtccgtgc gcgtggttct ggcgctcggc cggatcgcac atgacagcgt cttgctcgcg
    16261 cagggcctga aacgcagcgc ctttccgttt gcccacggtc aggagcattc tctcgctgac
    16321 ggacgttacc tgatcgactc atatcattgc agccgctaca acacccagac ccgcgtcctg
    16381 acggcgccca tgttcaagca ggtggtggcc cgagcctgca cgctggcgga gctctcgggg
    16441 ccgacctctc gcgcacgatg accgacacga cgttcgatgc aaaagagttc gtcggctccc
    16501 tgccgaggcg tcctggcgtc taccggatgt acaacgccgc gcacgagatc ctctacatcg
    16561 gtaaggccag aaacctgcgc gaccgggtgg gcacctattt cgccgcgagc aatgtcaacc
    16621 cgaagatcca ggcgctggtg cagcagatcg ccgccatcga ggtcacggtc tccaactccg
    16681 agaccgaggc gctgctgctc gaatacaacc tcatcaaggc gcacaagccc cgcttcaacg
    16741 tggtgctgcg cgatgacaag agctttccgt acatccaggt ctcgaccgat cacgaatttc
    16801 cgcgcctggc gttctatcgc gggccgcgca gtgcgccggg ccgctacatg gggcccttcc
    16861 ccagcgccgg cgcggtcaag gacaccttgc gcgagctgca gaagctgttt cgcatccgca
    16921 attgccggga cagcttcttc gccaaccgca gccggccttg cctgcaacat cagatcggcc
    16981 gctgttcggc cccctgtgtg gggctcatca cgcgcgaggc atatgcccag gacatcgacg
    17041 ccgcggtcaa ggtgctcgaa ggtcgcgatg ccgaagtgac tgcgcagctg cagcagcgga
    17101 tggaggccgc cgccgcacag ctggatttcg agcgcgctgc gcagattcgc gatcagctgg
    17161 cggcgttgaa gcggatcctt gcgcagcaga tcgtgacgga cgacggcgag cgcgatgtgg
    17221 acgtattcgc catcgcaggt gagccggggg aatacgcggt cagcgtcatg ctggtgcgcg
    17281 gtggccgcaa tctcggcacc agcagttact tcccccgcgg ggcattggcc gagccggaag
    17341 aggcattgtc atcgttcgtc atgcagtact acgccacgca ggagccgcca ccgcaagtgc
    17401 tcctcggccg caaactcgaa gatgcggatc cgctctccca ggcgctcggc ggccgcgtcg
    17461 gccgacgtgt cgaggtgcga tgtccggtgc gcggtctggg cgttcggtat gtggagcttg
    17521 cacgcgaaaa tgctgatcag gctttgcgca tgaaacttgc ccaacgacag ggtatccagg
    17581 agatgctggc ggatcttgcg cgggagctgg atctgcccga agtgcccggg cggatcgagt
    17641 gtttcgacat cagtcacacg ggtggggagg gcacagtggc ttcctgcgtc gtgttcggcc
    17701 ccgagggtgc gctgaagaag gagtatcgcc gtttcaacat ctcgggtgtt gcaccgggcg
    17761 atgactacgg ggcgctgcgc caggcgctcg agagacgtta tgtccggatc cggcagggcg
    17821 aggtcccctt gccggacctg ctgctgatcg atggcggcca gggacagatc gctctggtgc
    17881 acgaggtgct cgaggggctg gggttcggcg atcttgcgct cgtgggcgtc gccaaggggc
    17941 cggatcgacg gcccggacag gagcggctgt tcatccacgg agtccccgag ccacgcattc
    18001 cggaggctca ctcgcccgcc tcgcggctcg tgcagcggat acgggacgag gcgcatcgct
    18061 tcgcgatcac cggccatcgg cgcaagcgcg cgcgccgtta taacgaatcg gtgctggaag
    18121 ccataccagg tctcgggccg gcgaagcggc gggcgctcct gaagcatttc ggtggcatgc
    18181 agggcgtact gcgtgccggc accgccgatt tcgagaaggt caccggaatc ggggcggcgc
    18241 tcgcccgaag cctttatgat cacctgcatc cgggccagtg agtctcgtgt cgcgcccggt
    18301 tggtgagaac ttgtctacac acacgctgcc cgccaagcct gcggtacgct ggaactggcc
    18361 gaacacactg acgtggctgc ggatcgcagc gattccgctc atcgtcatcc tgttctacat
    18421 gccttatccg tgggccgacc cctcagcagg cctgctgttt gcggccgcgg gtatcaccga
    18481 ctcgctcgat ggctacctcg cgcggaggct gggtcagacc tcgaggctcg gcgccttcct
    18541 ggacccggtg gccgacaaac tcatcgtggc ggtcgctctc gtcctgctgg tcagcaagga
    18601 tcaccgtgcc ctcatcgtgc tcactgcggt agtcatcatc ggacgcgaga tcacgatttc
    18661 agccctgcgc gagtggatgg ccgagatcgg cgagcgccgg aaagtggccg tctcgcagct
    18721 cggcaagtac aagacgattc tgcagatcgt cggcttgtct ttcatgctgt tccggcgtga
    18781 gctgtggggc ttgcccatct accgcatcgg cgtggtgctg acagagctcg ccgcggcagc
    18841 aactctgtat tcgatggtgg cgtacctgcg cgcggcgtgg cctgagctga aacgttagac
    18901 ccgacgccac gacgggcata cgtgcatccg gatgggcgtg acggaccgct aagtaccgat
    18961 ggccattcgg ttttattctc gtgcgtgccg gggtcttacc ttgacttcgc gcagtgcaca
    19021 catacaatca cccctcgctg atcgggtgcg ggaatagctc agttggtaga gcacgacctt
    19081 gccaaggtcg gggtcgcgag ttcgagtctc gtttcccgct ccaaaatctc caggccggcc
    19141 tcgcttcgca gcctcgccgg ggccggacaa tccggccacc cgcccgcatt atcacttcgc
    19201 gcccttgggc tcgtccctca tcccttcgac ctggatacgc aggttcacct ccatcttgaa
    19261 gccgtagtcc ttgcccgcgc tcaaaccgaa ctggtcacgt tggaaagttg cgaacgcgtc
    19321 cgctccgcac cattctcgct tcaggagcgg atgcggcttg catgcgaacg aggtcagtgt
    19381 gagggagacg ggccgcgtga tgccgtggag ggtcagctcg ccgtcaacct gcgtcggcac
    19441 gtcgccgttg aaatgagtga agcggccgac gtaagcggcc tggggatact gagaggtgtt
    19501 gaggaactcc gggcccaccg cccatgaatt gagcgcatcc agaccgaagt cgatactggc
    19561 cggatcgatg gtgatattga cagtgccgct gtggcccgcg cggtccagtg tcacggtccc
    19621 cttcgaatgc ttcatcttgc cgcgccacgt ggacacaccg aagtgatcgg cttcgaagct
    19681 tggataggtg tgcatcgggt cgagcgtgta gtggacttcc tcagctgaga gagtcgatgc
    19741 accgagcagg gccgcgagtg cggtcagcat ggcggcgcgt ttcaacggaa cctttcgggt
    19801 ggtcatgtca gtgttcctat tcgcggaggt ccggcggcga gctttcgtct gtcatctgct
    19861 gcaaggacgc gtgtggggag cgtgagacga caagcggtgc agattttttc gggccgctgc
    19921 ggcgagaggt ggagtgagcg atcaggcacg ctggcccaat atctttattt cactgaaata
    19981 ttaaatcata actctatgat taaatgatac tttatctagc cttgcgtgca cgggatgtta
    20041 gccatcgctc acttgcacca atctgcttcc taaaccgcag ggaaagttcg gccgcaagcg
    20101 gcaagcttgc ctgatcaaga gaagcgctcg ctagagcttc ggagaactct gcccaccgtg
    20161 cgagcgcagg cgcgtcgcac ggcagagcta agttacgcag aacgccgcct ccgagcgctc
    20221 tgcagcggtc gcctgttcgc atcggggtca cgtgctaaga ttcgccgctc gagtgctgac
    20281 agagttcggc gcggcacttc ggatcgcgtg gcaatggtcg tcatggcgcg gtccatacga
    20341 tcccacaagc cgcgccgacg aagcctcgcc gattccaccc gtatggctag gtggcagagt
    20401 ggtcatgcag cggcctgcaa agccgtgtac gccggttcga ttccgaccct agcctcaaat
    20461 aaatccttcg cagacgttcg ccagcaacct caaataccga gaaaacctag aacaacacga
    20521 cgtttcatgg ctcgccggcc tgcgcagcca ttcgggggca gccggcccag gaggagggta
    20581 actttgcggg gtaaccgcga atcagctata aacgggctgc tagttacccc agggtgcagt
    20641 taccccacag gaccctccca tgccccagct caccgatacc aagatccgca acgcgatcaa
    20701 agaggcggtc aaaactcaga ggcgccgaaa gctctacgat caactcggcc tgtacctgat
    20761 tctggtgccg cgcgaaaaag ggtgcggatg ctggtggcgg caggactttg attggaacga
    20821 tcgagagcgg acgctcagcc tgggcgtata ccccgatatc ggcatcaagg cagctcgcca
    20881 gcggcgcgac gagatccgcg cgcaggtcgc gaacaacgtt gacccctcca ctgcacgtcg
    20941 agagaagaag gccgctcagc gcgactcctc agagcgcaca tatgaggtga tcgcgcgtca
    21001 gtggctcgat ctcacggcca agagccgcca ctggactgcg gatcacgtag agcgggtccg
    21061 ccggcgtcaa gaggctcacg tctggccgtg gctcggtcgc aggccggtcg ccgagattac
    21121 cgaggaagac gttctctcct gcctgaagcg tgtgacagac aagaacctga tcgataccgc
    21181 ccaccggctg cgcgccgata ccgacgggat ctttcggtac gccaagaaat ggaagttcat
    21241 caagagcaac ccaatggccg acctgcgtga ccgcgatacg ctgcccaaac tgaagacgac
    21301 ccacaatgca gccatcacaa acccgtctgc cctgggtacg ctgctacgcg ccatagacgg
    21361 ctatcccggg agcttcatcg tcaaatgcgc gctcaagcta caggcccttg tcttcgtgcg
    21421 acctggcgag ctgcggctcg cgacctggga tgagttcgat ctggacggtg cagagtggcg
    21481 catcccagcc aagcgtatga agatgcgaat tgagcacgtc gtgccgctgg ctcgccaggc
    21541 ggtcgcaatc ctccgtgacc tgtatccgct gactggcccc gatggcttcg ttttcccgca
    21601 ggtccgcagc ccattccgag ggatcagcaa taacacgctc ggcgcagccc tgcgcacgtt
    21661 ggggttttct gctgaccagc agtccgcgca tggcttcagg cgcaccgcga gcacgctgct
    21721 caacgagtcc ggaaagtggc atcctgacgc cattgagcgc cagctcgcgc acaaagagcc
    21781 caacgaaacc cgcgaagcct ataacgctgc ccagcatcta ccgaagcgcc ggaagatgat
    21841 gcaggcatgg gcggatcagc tcgacgcatt gaggaagggt agggcggcaa gcggagatgg
    21901 tgccgcgata gaagggagcg cgaatccggc tgcgctgtaa tgcgcgcctg tcacgcattc
    21961 aagaatgcgt gaccgggtac gcgaacggag caagtaatca gcatgatttc atggcactat
    22021 cattcgtaga gatacgtgga tggtgcccat gaacgacgac ggcaaggtgg ttgatcccaa
    22081 tgtcaggctg ctgaccgctg gagagcgcga ggaaattgct cagcactggc agcagtccgc
    22141 cgacaagagc ccgcgatacc agaatggccg cgaggtcgca ccgcccaggc cgatgacggc
    22201 ggagcagcta caggtcatat gcgatcactt ctggcgccag atgccagcgc cctcgtccgt
    22261 tgatcctttt tccctgcgag tggtcgcgaa gcatcagaag aacagatcgg ttcggccagt
    22321 gctggttgcg agaatggaga gactggggca tctcgaagga aagcggcaga gagagcgcgc
    22381 gaaggcggag caagctgcaa ttgaccatta ccgcgcgtgg gagacccgca tggctcatct
    22441 attggcaggg aaggatgccc gcgagcgcgt gaaacggtac aaacagtccg acgcgttcag
    22501 cagggcgcca acccggcaaa agcggcggat tacccccctt ctcaagtctg ggaagctgaa
    22561 tcttcaatag ccccgtagat ttcctgtacg gggtggcccg cccgttacca gttcgcgggc
    22621 tttaattagt ccatcgccag cgcccgctgg catctactag atggacacaa ctcaatgcca
    22681 attcgactga taaggctccc gcgagtctgc gacctgcgcg gtgatagacc gacttctgtt
    22741 tacgcggccg tgaaacagga attactcact ccgccgatca agctgacaaa gcgcgccagc
    22801 gcatggcccg agcacgaagt tgaggctgtc aacgccgcca agctcgccgg caagtcggat
    22861 gctgagatcc gcgatctcgt gaagatcctg accgccgcac ggcgcgaatt gctgccgtcg
    22921 ctgctggcga ccatcccgca ggtcgccgcc gcctgatcca attcgggctt gcggaaccct
    22981 tccccaaaaa ggaagggccg ccgtgctagc aacacgaaac ggcccaagga cgactcaatg
    23041 caactgtacg acgaactgga ccgcctggtc cagcaatccg aactgcgaac gctcgcaaga
    23101 ctgatacgcg gcgggctggt tcccatcgac ctcgatgacg ccattcccgg ctacggtgcc
    23161 ccgatcgtgg accagggata cgttccctcc cgcatcatcg aaattctgac gggtcgagat
    23221 caacccgacc gcagaccgtc tggtgtctgc cccgagtgtt atagcgaatg gcggcgcaga
    23281 aagctccggc ctcgcacggt gcgttgcgaa caccaacacg ctgccgcaca tcgtagaacg
    23341 atcagggttc gagacttctt cggcaaggtg acggaaaagc gcgtttgggc tgtcacccag
    23401 ggaatcgccc gggccaagaa tcagaaagag cttgcgcagc acatttgccc ccgctgcctg
    23461 cccgttggag gcgacgcaag tttggttgtc tgccggcatc gcggcacggc agcctaccgc
    23521 aagcgccgca cctggcgggt cagcctgggg ctcacgcaga aggagctggc gactctcaag
    23581 gagctggcgt ggtgagcgcg agcatcgtgc agctccatca catcgcagag agtcatctgc
    23641 gatgtgatgt c
//
LOCUS       DASRHN010000194         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2372645, whole genome shotgun sequence.
ACCESSION   DASRHN010000194 DASRHN010000000
VERSION     DASRHN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372645"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFB37_07620"
     CDS             complement(<1..839)
                     /locus_tag="VFB37_07620"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO22360.1"
                     /translation="MLQPAPDAGAAPSRAGTRTRVLTRRSVFGWAMYDWANSSFATTV
                     MAGFFPLFFKQFWSAGVPATQSTFRLGLANGVASFLVALMAPLIGAIADKGRARVRLL
                     GLFTVLGAAMTAGLYFVGKGDWQSATLLYILASLGFWGGNQFYDSLLTDVSEEHEYDL
                     VSGYGYALGYLGGGLLFALNVSMVLKPALFGLPDAGAAVRWSFVSVGVWWSVFTIFTV
                     TLVRERPTHTPLPAMAAIRAGAAELTHTLGLLRGERVVLWFLLAYWFYIDGVNTIIKM
                     AVD"
     gene            complement(862..2454)
                     /locus_tag="VFB37_07625"
     CDS             complement(862..2454)
                     /locus_tag="VFB37_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003177920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZO22361.1"
                     /translation="MSDARVAEEIRRRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAG
                     TVKGRKAARHATSDWMRLEQQRGISVTSSVMQFPFRDCIVNLLDTPGHEDFSEDTYRT
                     LTAVDSALMVIDCAKGVEERTIKLMEVCRLRDTPIMTFINKLDRDGRPAIELLDEIES
                     VLRIRTAPITWPIGMGRELRGIYHLLEDRIYAYTAGERGRVGENRAIDGLGSAQGREF
                     LGDLAAAFDEEIALVKGATEAFAVEEYRAGRQTPVFFGSAISNFGVEELLSSFARHAP
                     GPQPRAAREREVDPLEPDLTGFVFKIQANMDPMHRDRIAFLRLCSGQYTRGMRLHHAR
                     LAKEVRVADALTFMAAERAQAEEAYAGDIIGLHNHGTINIGDTFTGGEQLAFTGVPNF
                     APEIFRRAVLKDPLKMKALSKGLSQLCEEGATQLFKPLRNNDLILGAVGQLQFEVVAF
                     RLQDEYGVQCVFEAINVHTARWVESDDERKMEEFRSRAHDHLAVDHSGALVYLAPSRV
                     NLQLTQERWPEIRFHETREHAM"
     gene            complement(2447..2944)
                     /gene="rimI"
                     /locus_tag="VFB37_07630"
     CDS             complement(2447..2944)
                     /gene="rimI"
                     /locus_tag="VFB37_07630"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZO22362.1"
                     /translation="MATAPELFDVVPDTLIRPMVESDVLDVVAVERASYQFPWSEGIF
                     RDCLRVGYVCRVVIVAEQVIGYGVMSVGAGEAHILNLCIAEVFRNRGIGKRLLAYLIE
                     RGSAAGMSEAFLEVRPSNAAALRLYLSHGFEQVGMRRGYYQAVGGREDAAVLKLTLRS
                     RRARV"
     gene            complement(2958..3704)
                     /locus_tag="VFB37_07635"
     CDS             complement(2958..3704)
                     /locus_tag="VFB37_07635"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZO22363.1"
                     /translation="MDSRRRNEYLEAIGIDVWVSRHAEAVEPHSVAEVPVSRVAAPAP
                     AGGAEDRRERAPGVGDADWGALRAQVMSCTRCPLHATRTQGVVGVGSHTAPWLVVGEA
                     PGAEEDRRGEPFVGRAGHLLDAMLRAIGLSRGTNVYIANILKSRPPGNRDPKPEEVAA
                     CLPYLMRQLQLLQPRLMLAVGRIAAQNLLGTDAPLGRLRGRVHHFGELNTPLIVTYHP
                     AYLLRTPGDKRKAWEDLKFARSVFRQLRPS"
     gene            complement(3704..4531)
                     /gene="pssA"
                     /locus_tag="VFB37_07640"
     CDS             complement(3704..4531)
                     /gene="pssA"
                     /locus_tag="VFB37_07640"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZO22364.1"
                     /translation="MPDRPSGVRTRAHVPMQMEVEEELKPRRKGIYLLPNLLTTGCLF
                     SGFYAIVASIDKHFEQAGIAIFVAMLFDTLDGRIARLTKTESAFGKEYDSLADMVAFG
                     LAPAIVAYQWGVVRIAEFGRYWGRFGWLACFFYAVAAGMRLARFNTRAAADKRYFEGL
                     PSPSAAAIVAAFVWFSIHFREPGLVGLIAAFVITASAGALMVSRFSYPSFKQFNLDRR
                     IKFAYMLMVPLFFVLIAINPSAMLLSMFSTYALSAPVLWVGRRIRRLFRSTPAPGPS"
BASE COUNT          879 a         1589 c         1471 g          762 t
ORIGIN      
        1 tagtccaccg ccatcttgat gatggtgttc accccgtcga tgtagaacca gtaagcgagc
       61 agaaaccaca gcacgacgcg ctcgcctctg agcagcccga gggtgtgggt cagctctgca
      121 gcgccggctc gaattgccgc catagcaggt agtggcgtgt gggtcgggcg ctcgcgcacc
      181 agcgtgacgg tgaagatcgt gaacaccgac caccacacac caaccgatac gaaggaccag
      241 cggaccgccg cgccggcatc cgggaggcca aacagcgccg gcttcagcac catgctcacg
      301 ttgagtgcga acagcagccc gccgcccaga tagcccaggg cataaccgta cccggagacg
      361 aggtcgtatt cgtgctcttc ggagacgtcc gtgagcagcg agtcgtagaa ctgattccca
      421 ccccagaatc ccagcgatgc caggatgtag agcagcgtgg cgctctgcca gtcgcccttg
      481 ccgacgaaat acaggcccgc ggtcatggcg gcaccgagca cggtgaacag cccgagcagg
      541 cgcacccgcg cacggccctt gtccgcgatg gcgccgatca gcggcgccat cagagcgacc
      601 aggaagctgg ccacgccgtt ggccagcccg agtctgaaag tgctctgcgt cgccggcacg
      661 ccggcgctcc agaattgctt gaagaacagc gggaagaagc cggccatcac ggtcgtggcg
      721 aaagaggagt tcgcccagtc atacatcgcc cagccaaaca cggagcggcg ggtgagcacg
      781 cgagtgcgcg tgcccgctcg cgacggcgcc gcgcccgcat cgggcgccgg ttgcagcatt
      841 ccgctcacat ccgggcctca ctcacatcgc atgctcgcgc gtctcatgga agcggatctc
      901 aggccagcgc tcctgcgtca attgcaaatt gacacgtgag ggggcgagat acaccagcgc
      961 gccactgtgg tccaccgcca gatgatcgtg cgcgcggctg cggaactcct ccatcttgcg
     1021 ctcgtcatcg ctctccaccc agcgcgccgt atggacattg atcgcctcga acacacattg
     1081 cacgccatat tcgtcctgga ggcgaaaggc gacgacctcg aactgcagct ggcccaccgc
     1141 cccgagaatc aggtcgttgt tgcgcagcgg cttgaagagc tgcgtggcgc cctcctcgca
     1201 cagctgtgac aggcccttgc tcagcgcttt catcttcaaa ggatccttga gcacggcgcg
     1261 tcgaaagatc tccggcgcga aattcggcac ccccgtgaaa gcgagctgct cgccgcccgt
     1321 gaaagtatcg ccgatgttga tcgtgccatg gttgtgcaga ccgatgatat cgcccgcgta
     1381 ggcctcctcc gcctgggcgc gctctgcagc catgaaggtc agcgcatccg cgacacgcac
     1441 ctccttggcg agcctcgcgt gatgcaaccg catgcctcgc gtgtactgac ccgagcacag
     1501 ccgcaggaag gcgatccggt cgcggtgcat gggatccatg ttcgcctgga tcttgaacac
     1561 gaagccggtc aggtccggct cgagcggatc gacctcgcgc tctctcgccg ctcggggctg
     1621 aggtccagga gcatgccggg cgaaggagct cagcagctct tcgacgccga aattactgat
     1681 ggcggagccg aagaacacgg gcgtctgccg cccggcgcga tattcctcga cggcgaaggc
     1741 ttccgtcgcc cctttcacga gcgcgatctc ctcgtcgaag gcggcggcca gatcgcccag
     1801 gaattcgcgc ccttgcgcac tgcccagtcc gtcgatggct cgattctcac cgacccgacc
     1861 ccgctcgcct gcggtgtagg cgtagatgcg atcctccagc agatggtaga tgccgcgcag
     1921 ctcgcgcccc attccgatcg gccaggtgat gggggcggtg cggatccgca ggacgctctc
     1981 gatttcatcg agcagctcta tggctggccg gccatcgcga tcgagcttgt tgatgaaggt
     2041 catgatcggt gtgtcgcgca ggcggcagac ctccatgagc ttgatggtcc gctcctccac
     2101 tcccttggcg caatcgatga ccatcagcgc ggaatccacc gcggtcagcg tccgataggt
     2161 atcctccgaa aaatcctcgt gccccggcgt gtccagcaga ttcacgatgc agtcgcggaa
     2221 cgggaactgc atcaccgagg aggtcaccga gatgccgcgc tgctgctcga ggcgcatcca
     2281 atccgaggtc gcatgccgcg ccgctttgcg gcccttcacc gtgccggcca tctgaatggc
     2341 gccgccgaac agcagcagct tttcggtcaa cgtggtcttg cccgcatccg ggtgggagat
     2401 gatggcgaag gttcggcggc gcctgatctc ttcggccacc cttgcgtcag acacgagccc
     2461 ggcgactccg caaggtcagt ttgagcacgg ccgcatcctc gcgtccgccg acggcttgat
     2521 agtagccgcg ccgcatgccg acctgctcga agccgtgtga caggtacaaa cgcaaggccg
     2581 cagcgttcga tggacgcacc tcgaggaagg cctcgctcat cccggctgcc gagccgcgct
     2641 cgatcagata ggccagcagc cgcttaccga tgccacggtt gcgaaatacc tcggcaatgc
     2701 acaggttcaa tatgtgcgct tcaccggcgc cgacgctcat gacaccgtag ccgatcacct
     2761 gctcggcgac aatcacgaca cggcacacgt agcccacccg caggcagtcg cggaaaattc
     2821 cttcgctcca cgggaactgg taagaggcgc gctcgacggc aaccacgtcc agcacgtcgc
     2881 tctccaccat cgggcggatc agcgtgtccg ggacgacatc gaacagctcg ggggcggttg
     2941 ccatcggtgc ctgtgaatca actgggcctc aactgcctga acacgctgcg cgcgaacttc
     3001 aggtcttccc acgccttgcg cttgtcgccg ggggttcgca ggaggtaagc aggatgatac
     3061 gtcactatga gcggggtatt cagctcacca aaatgatgca cccggccgcg caaccgtccg
     3121 agcggtgcat cggtccccag caggttctgg gccgcgatcc gccccaccgc gagcatgagc
     3181 cggggctgca gcagttgcag ctggcgcatc aggtacggca ggcaggcggc gacctcttcc
     3241 ggcttcgggt cgcgattgcc gggcgggcgg ctcttcagaa tattcgcgat atacacgttc
     3301 gtgccgcgac tgagcccgat ggcacgcagc atggcatcca ggaggtgccc agcgcggccc
     3361 acgaaaggct cgccgcgcct gtcctcctcg gccccggggg cttccccgac caccagccag
     3421 ggggccgtgt ggctgccgac gccaaccacg ccttgcgtgc gcgtcgcatg cagggggcag
     3481 cgggtgcagc tcatcacttg cgcgcgcaaa gcgccccaat ccgcatcccc aacccccggc
     3541 gcgcgctccc gccggtcttc tgcacctccc gccggggcgg gggctgccac gcgggagacc
     3601 ggcacctccg ccacggagtg gggctctact gcctcggcat gccgtgatac ccagacatcg
     3661 atcccgatcg cttccaggta ttcgttgcga cgccggctgt ccatcaactg gggccgggag
     3721 cgggagtgct ccgaaagagc cgtcggatcc gccttcccac ccacagcacg ggggcggaca
     3781 aggcgtaggt cgagaacatc gacagcagca tggccgatgg attgatcgcg atgaggacga
     3841 agaagagcgg gaccatcagc atataggcga acttgatccg ccggtccaga ttgaactgct
     3901 tgaagctcgg atagctgaag cggctcacca tcagggcccc cgcgctcgcc gtgatcacga
     3961 aggcagcgat caggcccacg agacccggtt cgcggaagtg gatcgagaac cagacgaaag
     4021 ccgccacgat ggcggcggcg gaggggctcg gcagaccctc gaaatagcgt ttgtccgcgg
     4081 ccgcccgcgt gttgaagcgc gccaggcgca ttcccgccgc cacggcatag aagaagcacg
     4141 ccagccagcc gaagcggccc cagtaccgcc caaactcggc gatccggact acgccccact
     4201 ggtaggcgac gatcgccggc gcaagaccga aggcgaccat gtccgccagg ctgtcgtatt
     4261 ccttgccgaa cgcactctcg gtcttcgtga gacgggcgat tcgcccgtcc agggtatcga
     4321 acagcatcgc cacgaagatg gcgatgcccg cctgctcgaa gtgcttgtcg atcgaggcga
     4381 cgatcgcata gaagccggag aacaggcagc ccgtggtgag cagattcggc agcagataga
     4441 tgcccttgcg gcgcggcttc aactcctctt cgacttccat ctgcatgggc acgtgcgctc
     4501 tggtgcggac accgctgggc cggtcgggca tcttatcagc taagatgctg ttttacggcc
     4561 cggcgaccgt gcctgcccgc gtggaccctg tcctgcggac ccttcccgat tcgaggacac
     4621 cgagcgcgcg atgaggcttt cgcagtaccc gatcaacact ctcaaggagg ttccggcgga
     4681 agccgaggtg gtgagccagc a
//
LOCUS       DASRHN010000195         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2372697, whole genome shotgun sequence.
ACCESSION   DASRHN010000195 DASRHN010000000
VERSION     DASRHN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372697"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /gene="gcvPA"
                     /locus_tag="VFB37_07645"
     CDS             <1..1132
                     /gene="gcvPA"
                     /locus_tag="VFB37_07645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZO22365.1"
                     /translation="ITTRGEFYSAYTPYQAEASQGTLQLIYEYQTMMAGLTGMDVSNA
                     SLYDGASAMAEASLMAVRANRKSKSLRILTPTSVNPRYRKVATATAGNQGLRFEELPH
                     DPATGCVGDKTLQKLPSEDCTALIIQQPNFFGQLEDVDTLTDWAHARGMLAIAVVNPT
                     SLALLKPPGQWGSRPGAGADIVVGEGQPLGVPLSSGGPYFGFMTTRMEYVRQMPGRIV
                     GRTVDLSGHPGFTLTLQAREQHIRRARATSNICTNQGLLMTAATIYLALMGATGLQRT
                     AVASHARTVELVRALQSVKGVRQAFSGPFFHESVLQLDRPVAPLLQFLASRGILGGLD
                     LSTYYPELGHALLVCATETKTEADIERYAAVLGEAMQAARAA"
     gene            1193..>2239
                     /gene="gcvPB"
                     /locus_tag="VFB37_07650"
     CDS             1193..>2239
                     /gene="gcvPB"
                     /locus_tag="VFB37_07650"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZO22366.1"
                     /translation="MRSHEKLIFEYSHPGRGASDQWPEELPASATADLPAHLRRTGAP
                     VLPEVSELETVRHFTRLSQLNFSIDTHFYPLGSCTMKYNPKACNQYAMLPGFLGRHPL
                     APESHGQGFLACMYELQEMLKEVTGMQAVALSPMAGAHGEFAGVAMIRAYHRARADLG
                     RNEIIVPEAAHGTNPATATMCGCVVREVPVSADGDIDLDALKAAAGPNTAGIMLTNPS
                     TLGIFERRIEEVARIVHAAGGLLYYDGANLNAILGKVRPGDMGFDVMHMNLHKTFSTP
                     HGGGGPGAGAVGVGARLQPFLPIPIVGLVAGKEQDGTGERYRWMAEKNFPQSIGRLSA
                     FMGNAGVLLRAYIYM"
BASE COUNT          403 a          736 c          723 g          377 t
ORIGIN      
        1 catcaccacg cggggtgagt tttacagcgc ctacacgcca taccaggccg aggcgagtca
       61 gggtacgctg cagctgatct acgaatacca gaccatgatg gcggggctga cgggtatgga
      121 cgtctcgaac gcctcactat acgacggagc atccgcgatg gccgaggcga gcctcatggc
      181 cgttcgcgcg aatcgcaaat cgaagtcgct ccggatcctc acacctacca gcgtcaatcc
      241 gcgctacaga aaagtcgcga ccgccaccgc gggcaaccag gggctgcgtt tcgaggagct
      301 cccccacgat ccggccacgg gctgtgtcgg cgacaagacg ctgcagaaac tccccagcga
      361 ggattgcacc gcgctcatca ttcagcagcc caacttcttc ggacagctcg aagacgtgga
      421 caccctgacc gactgggcgc acgcgcgcgg catgctggcg atcgccgtgg tgaatccgac
      481 ttcgctggct ctcctcaagc cgcctggcca gtggggcagc cggcccgggg cgggcgcaga
      541 catcgtcgtg ggtgaggggc agcctctggg agtgcctctg tcttcgggcg gaccctactt
      601 cggtttcatg accacgcgca tggagtacgt gcggcagatg ccgggccgca tcgtcggccg
      661 gacggtcgat ctctcgggac acccggggtt cacactgacg ttgcaggctc gcgagcagca
      721 catccgccgc gccagggcca cttccaacat ctgcaccaat cagggactgc tgatgacggc
      781 cgcgacgatc tatcttgcgc tcatgggtgc cacagggctg cagcgcacgg ccgtggcctc
      841 tcacgcacgc acagttgagc tcgtgcgggc gctgcagagc gtgaaaggag tgcggcaagc
      901 cttcagcggg ccctttttcc acgagtcggt cctgcaactc gaccggccgg tagcgcccct
      961 gctgcagttt ctggccagcc gcggcattct cggcggactc gatctatcga cttactaccc
     1021 ggagctcgga catgcgctgc tggtctgcgc gaccgagacc aagaccgagg cggacatcga
     1081 gcgctatgca gccgtcctgg gagaggccat gcaggcggcg cgagcggcct gacccgcctt
     1141 gccaagtctg aattcgagga cgcgagactg ctgacgagca acgggatcat tcatgcgatc
     1201 acacgaaaag ctgatcttcg agtactccca tcctggccgg ggcgcatccg accagtggcc
     1261 cgaggagctg cctgcctccg ccactgccga cctgcctgct catttacgcc gcaccggggc
     1321 gccggtgctg ccggaggtga gcgagctcga gacggtgcgg catttcaccc gcctgtcgca
     1381 gctcaacttc tccatcgaca cgcatttcta cccgctggga tcgtgcacga tgaagtacaa
     1441 ccccaaggcc tgcaatcagt acgccatgct gcccgggttt cttggccgtc atccgctcgc
     1501 gccggagtcg catggacagg ggtttctcgc gtgcatgtac gagctgcagg aaatgctcaa
     1561 ggaagtgacc ggcatgcagg ccgtcgccct gagtccgatg gctggggcgc atggcgagtt
     1621 cgcaggggtg gcgatgatcc gcgcatacca ccgcgcgcgc gcagatctgg ggcggaacga
     1681 gatcatcgtt ccagaggcgg cgcacggcac caacccggca accgcgacga tgtgcgggtg
     1741 tgtcgtgcga gaggtcccgg tgagcgccga cggcgacatc gacctcgatg cgctcaaggc
     1801 ggccgccggg cccaacaccg ccggtatcat gcttaccaac ccttcgacgc tcggtatctt
     1861 cgagcgcagg atcgaggagg tcgcgcggat cgtgcacgcc gccggcggcc tgctctacta
     1921 cgacggcgcc aatctcaacg ccatcctggg caaggtcaga ccgggggaca tgggcttcga
     1981 tgtgatgcac atgaacctgc acaagacgtt ctcgacgcct catgggggcg gaggtccggg
     2041 tgctggggcg gtcggcgtcg gcgcgcgcct gcaacccttt ctgcccatcc cgatcgtcgg
     2101 cctcgttgca ggcaaggagc aggatggcac aggcgagcgc taccgctgga tggccgagaa
     2161 aaattttccc cagtcgatcg gcaggctgtc cgcgttcatg ggcaacgccg gcgtgctgct
     2221 gcgtgcctac atctatatg
//
LOCUS       DASRHN010000196         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2372973, whole genome shotgun sequence.
ACCESSION   DASRHN010000196 DASRHN010000000
VERSION     DASRHN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372973"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VFB37_07655"
     CDS             complement(<1..892)
                     /locus_tag="VFB37_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZO22367.1"
                     /translation="MRAQRIAPAVAVSWLTLGAMLALGRSGPLTAAQGRPPALAAQAS
                     HVSITRDDWGIAHVKGHTDADAVFGMIYAQAEDDFNRVETNYLVSLGRLAEAEGESAI
                     WQDLRQRLYIDPTQLKADYEHCPAWLKQLMQGWAAGLNEYLATHPQVHPRVLTHFEPW
                     MALSFSEGSIGGDIERVPLSQLQAFYEHKTLAMTPSERGVMLSDPQGSNGFAIAPSHT
                     RDGHALLLINPHTSFFFRSELQMTSDEGLNAYGAVTWGQFFIYQGFNTRVGWMHTPSG
                     VDNVDEFAETIVTAPDGSRAY"
     gene            1019..2224
                     /locus_tag="VFB37_07660"
     CDS             1019..2224
                     /locus_tag="VFB37_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO22368.1"
                     /translation="MKLQDAQQTVGHFLSPLTVDQFLDEVLLGGFAKIGGNRQAGRTD
                     LLGADPQSALLEAVHLAPKLTFHSANASGPPPSLAGVRDRADFQERIAEFHARHYSVR
                     FPELRPLSSALEHLGRALEALLHQPVTASAFWSRGGLRAPVHCDDHDLLVVQLRGAKR
                     WLIARKASGLENTWKGIPGEPPELGDHESLDLLPGDLLYLPRGTFHSVDSDVESLHLS
                     IGFTPLTVREAVIAALDHLSDADRTLRMTAGNRLAFQLRGAGIERFGPLVMESLARLA
                     GACRTPGFLNEALQRRSARAIGWLQATPAAACVTEISLDTVLVHRDTAFCHLTANPDK
                     IDFAYPGGHLYIHRGAEESVLYIANTTRFAVRDIPGGLRDDARLSLARRFVEVGFLEP
                     ASDAPAPSA"
     gene            complement(2267..3922)
                     /locus_tag="VFB37_07665"
     CDS             complement(2267..3922)
                     /locus_tag="VFB37_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZO22369.1"
                     /translation="MFTQLLDPLDNLALTCLVALIPVVLLLVLLAVFRWPAWLATLVG
                     SIVTFVLAQWVWKMPFNDGVHAYFYGAATGVWNVDWITFWGMVLFNTLAVSGVFENFR
                     RWLIAQGSMDVRVQTMLFAWAFGALLEGLVGFGYPWAVVAPILISLGISDLNAIRVAA
                     IANNAPVSYGALGAPIIALAAVTNYPLLSLSGSVGTVVAVLALLPPWVLLYLVSGKEG
                     MKGGWPLAIVGSLGYIAGQYPVAVHLGPYLPDVTGAIVCFIALLALLKVWQPKAVLGY
                     GGVPVDPALARQTQGHGLRPAEIAQAWMPFAVLLIVVAAWTGPWSPLPKVTWFSAKAV
                     ACSSLAATCSAAKPDVTAVFKFAPYVGGTAILASWILVAVLLLAMGKLKGAQIGEVFR
                     RTFHQMWGACLVGVFIFGLAYIFNYSGMAASLAKGFSSMGTAFIVVSPILGFIGVALS
                     GSNTSTNAMFGKFQALVATQLGMPPLLLPTLNSVGAEIGKPVAPQTASVGVSTSKFVR
                     KEGDVIRHNMGWTFILLAYLILIAIGCYLVHPASMMAATPATG"
BASE COUNT          821 a         1372 c         1369 g          730 t
ORIGIN      
        1 gataggctcg cgagccatcc ggcgcggtga cgatggtttc cgcgaactca tcgacgttgt
       61 ccacgccgct cggggtgtgc atccagccca ccctggtgtt gaatccctga tagatgaaaa
      121 actgacccca ggtgacggcg ccgtacgcgt tcaggccttc atcgctcgtc atctgcagct
      181 cagagcgaaa gaagaagctg gtatgcggat tgatgagcag cagcgcatga ccgtcacggg
      241 tatgcgaggg agcaatggca aagccgttgg atccttgcgg atcgctcagc atcactccgc
      301 gctccgacgg tgtcattgcg agtgtcttgt gctcgtagaa ggcctgcagc tgcgagagcg
      361 gcacgcgctc gatgtcaccg ccgatactgc cttcggagaa gctgagcgcc atccagggct
      421 cgaagtgtgt cagcacgcgc ggatgaactt gaggatgggt agcgagatac tcgttgaggc
      481 cggccgccca gccctgcatg agctgcttca gccatgccgg acagtgttcg tagtccgcct
      541 tcagctgtgt cggatcgatg tacagacgct ggcgcaaatc ctgccagatc gcgctctccc
      601 cctcggcctc cgccagacga ccgagactga cgaggtagtt ggtctcgaca cgattgaagt
      661 cgtcctccgc ttgcgcatag atcattccga agacggcatc tgcgtcggtg tggcccttca
      721 cgtgggcaat tccccagtca tcgcgggtaa tgctcacgtg cgaggcctgt gccgccaagg
      781 ctggcggtcg cccttgcgct gcagtgagcg gacctgagcg gcccagagcc agcatagccc
      841 ccagtgtcag ccaggacacg gcaacggcgg gagcgatacg ctgagctcgc atgggtcggg
      901 catcataaac cggcgcgcag cgcacgagtg acaagattcc tcgttgcctg gcgcattccc
      961 gggcgggcat actcgatgat gatcgaggct ttcagccgac gcacggcaga gcgatggcgt
     1021 gaaactccag gacgcgcaac agaccgttgg ccatttcctc tctccgctga cagtggatca
     1081 gtttctcgat gaggtcctcc tgggcggatt cgcgaagatc ggggggaatc gccaggcggg
     1141 ccgcacagac ttgcttggcg cggatccgca gtccgcgctc ctggaggctg tgcatctcgc
     1201 accgaagctg actttccact ccgcgaacgc atcgggccca ccgccctcgc tcgccggtgt
     1261 gcgtgaccgg gccgatttcc aggagcgcat cgccgagttt catgcccgac actactcggt
     1321 acgctttccg gagctgcgcc cgctgtcatc tgctctggag catctcggcc gcgcattgga
     1381 agcgttgctg catcagcccg tcacggcctc cgcattctgg agccgtggtg gcctgcgtgc
     1441 gcccgttcat tgcgacgatc acgacctcct ggtcgtgcag ctgcgcggcg ccaagcgctg
     1501 gctgattgcc cggaaagcct cggggctgga gaacacgtgg aaaggcattc cgggcgagcc
     1561 accggagctg ggagatcacg agtctctgga tctgcttccc ggggatctgc tttatctgcc
     1621 tcgcggcacc tttcacagcg tcgactcgga cgtcgaatcg cttcatctgt cgatcggctt
     1681 cacgcccctc acggtccgcg aggcagtgat cgcagcgctg gaccacttgt ccgacgccga
     1741 tcggaccttg cgtatgacgg cgggaaatcg cctggcgttt cagctccgag gggcggggat
     1801 cgaacggttc ggtccgctcg tgatggagtc tctcgcacgc ctggcgggtg cctgtcgaac
     1861 gccaggcttt ctgaacgaag cgctgcagcg ccgctcagcg cgcgccatcg gctggctgca
     1921 ggccacgcct gccgcggcgt gtgtgacgga gatcagtctc gacacggtcc tggtccatcg
     1981 tgacaccgcg ttctgccatc tgaccgccaa tccggacaag atcgacttcg cctatcccgg
     2041 tggacacctc tacatccatc gcggcgccga agagagtgtc ctctacatcg ccaacacgac
     2101 ccgattcgcg gtccgcgaca tcccgggagg tctgcgggat gacgcgcggc tgtcgctggc
     2161 gaggcgattc gtggaagtcg gctttctcga gcctgcttcc gacgcccctg ctccctcagc
     2221 ctgagggctc gccagcgctc aacagccgcg cgaagtgtcc ggtcactcat ccggtggcag
     2281 gcgtcgccgc catcatcgat gccggatgca caagatagca gccgatcgcg atgaggatga
     2341 ggtaagcgag cagaatgaag gtccatccca tgttgtgacg gatcacatcg ccctctttcc
     2401 tcacgaactt gctggtcgaa acgccgacgc tggcggtttg cggcgccacc ggcttgccga
     2461 tctccgcacc gacggagttc agggtgggca gcagcaaggg cggcatgccg agttgcgtgg
     2521 ccacgagggc ctggaacttg ccgaacatcg cattcgtcga ggtattggag cctgagagtg
     2581 cgacgccgat gaagcccagt atcggggaga ccacgatgaa ggcggtcccc atgctcgaga
     2641 agcccttcgc gagcgaagcg gccatgcccg agtagttgaa gatgtaggcg agaccgaaga
     2701 tgaacacgcc gaccaggcag gcgccccaca tctggtggaa agtgcgccgg aagacctcac
     2761 cgatctgcgc gcccttgagc ttgcccatcg ccagcagcag cactgcgacc aggatccacg
     2821 atgcgaggat cgcagtgccc ccgacgtagg gggcgaactt gaagactgcg gtgacatcgg
     2881 gtttggcggc gctgcatgtc gccgcaagcg aggagcaggc gactgccttg gcgctgaacc
     2941 acgtcacctt gggcaacggc gaccacgggc cggtccaggc ggcgacgacg atcagcagga
     3001 ctgcaaaagg catccaggct tgcgcgatct cagcgggccg caatccatgc ccctgtgtct
     3061 gccgggcgag cgcgggatcg accggaacac cgccgtaacc gagcaccgcc ttcggctgcc
     3121 agaccttcaa cagcgccagg agcgcgatga agcagacgat cgcgcccgtc acatcgggca
     3181 gataagggcc cagatgcacg gcaaccgggt actggccggc gatgtagccc aaggagccga
     3241 cgatggcgag cggccaaccg cccttcatcc cttccttgcc cgagaccaga tacagcagca
     3301 cccaaggtgg caggagggcc aggactgcaa cgaccgtacc gacggatccg gacagcgaaa
     3361 ggagtggata gttggtcacg gccgccagcg caatgatggg cgcgcccagc gcgccatacg
     3421 acaccggcgc gttgttggcg atggcggcca cgcggatcgc attgagatcg gagatcccga
     3481 gggagatcag gatcggagcg acgaccgccc agggatagcc aaagccgacc agaccttcga
     3541 gcagcgcgcc gaatgcccag gcaaacagca tggtctggac tcgcacgtcc atgctgccct
     3601 gcgcgatcag ccagcggcgg aaattctcga acacgccact gaccgccagc gtgttgaaca
     3661 gcaccattcc ccagaaagta atccagtcga cgttccacac gcctgtggcc gcgccataga
     3721 agtaggcgtg caccccatcg ttgaacggca tcttccacac ccattgcgcc agcacgaacg
     3781 tcacgatcga gccgacgagc gtcgcgagcc acgccggcca gcggaagacg gcgagcagaa
     3841 cgagaagcag gacgacggga atcagtgcta ccagacaggt cagagcgagg ttgtcgagcg
     3901 ggtcgagcag ttgcgtgaac atgacttctc cctggcttgg cgccgtgcga tggctgtggt
     3961 cgcaagaccg cgcgtcgcag cggttcgagt tcttgtcgtg acggagcagg cgggacggta
     4021 catcgacacc ggataaaatg caagaaaacc ctatgttttc cagcacttgg gtgtcgaacg
     4081 gagccggcgg cccacgctac gcgagggcta cggcattccg gcaggcggcg atgcgcggct
     4141 cggctgccgt tccccggcag ccgcacctct tcagaacctt agtccgatcg tgatcggcac
     4201 gaactcctgc ttgctcctca agtcgcccag gcgcatgtag cgcgcttcca cgaagaagct
     4261 cgtcgggtcc gtcagcgcga actcgaagcc ca
//
LOCUS       DASRHN010000197         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_892390, whole genome shotgun sequence.
ACCESSION   DASRHN010000197 DASRHN010000000
VERSION     DASRHN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892390"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="VFB37_07670"
     CDS             <1..1647
                     /locus_tag="VFB37_07670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO22370.1"
                     /translation="TQQYMTVSSVVSGVLLLLGMLGVILVLLSEAHEWAEAHWVNYRR
                     RLGAPALASGERQPKVSIHVPAYNEPPDMVIETLDALARLDYSNYEVLVIDNNTKDER
                     VWRPVEAHCARLGARFRFFHVAPLEGFKAGALNFALAHTAADAEIVAVIDSDYVVDPN
                     WLRDLVPGFQDPRTAIVQAPQDYRDESVSAFKAMCYAEYRGFFHIGMITRNERNAIIQ
                     HGTMTMVRRAQLQAAGWAEWCITEDAELGLRIFEAGFEATYVPTSYGRGLMPDTFIDF
                     KKQRFRWAYGAMQILKRHARAFFMEGGSLSPGQRYHFIAGWLPWVADGCNMLFNVAAL
                     GWSAAMVLDPHRIDPPLVMYSVLPLSLFTFKLAKLVHLYRVRVGANFRQTIAAAIAGL
                     ALTHTIGMAVVKGLVTGSEPFFRTPKTGQTARWVQALVAARGETFMMLGLWLSAWAVA
                     HSPQQHGPDRLAWIVVLLIQSVPYVSSLLASLASAFPLPARLLGTGYRPTSAASRPGP
                     GMPSGTPANAAGVQNAVGESGRSGPADHGAPEATNGPALL"
     gene            1865..>2390
                     /locus_tag="VFB37_07675"
     CDS             1865..>2390
                     /locus_tag="VFB37_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZO22371.1"
                     /translation="MLIAVAAFSGMDSVLKLFAAHYPPMEIAFLRGLASLPFMVLPAL
                     LKGRYRDLKPGRLGMHLLRGVLMVVMLWCFIYAVRTLSLANAYSIYLAEPLIVTALAV
                     PLLGDRVGWRNWVAICVGLIGVVVILRPSASGFVTLGSLAALVSATAYALSVIALRVI
                     TRTDTTTSVVVWTVG"
BASE COUNT          365 a          760 c          779 g          486 t
ORIGIN      
        1 acgcagcaat acatgacggt ctcgagcgtg gtatcgggcg tcctgctcct gctgggtatg
       61 ctcggcgtca ttctggtgct gctctcggag gcccatgaat gggccgaggc gcattgggtg
      121 aactaccgcc ggcgcctcgg agctcccgca ctggcaagcg gtgagcgcca gcccaaagtc
      181 tcgatccatg tgccggccta caacgagccg cctgacatgg tgatcgagac gctcgatgcc
      241 ctggcgcggc tcgactattc aaactatgaa gtgctggtca tcgacaacaa cacgaaggac
      301 gagcgcgtct ggcgtcctgt ggaggcccac tgcgccaggc tcggagcgcg gttccgcttc
      361 tttcatgtcg cgccactgga gggtttcaag gccggcgccc tgaatttcgc cctggctcat
      421 accgccgccg atgcggagat tgtcgcggtc attgacagcg actatgtcgt ggacccgaac
      481 tggttgcgcg acctcgtgcc gggattccag gacccgcgca ccgctatcgt ccaggcacca
      541 caggactatc gcgacgagag cgtgagcgct ttcaaagcga tgtgctatgc cgagtaccgc
      601 gggttcttcc acatcggcat gatcacgcgc aacgagcgta acgccattat ccaacatggg
      661 accatgacga tggtccggcg tgcgcagttg caagccgccg gctgggccga gtggtgtatc
      721 accgaggatg cggagctcgg cttgcggatc ttcgaggccg gtttcgaggc cacctacgtc
      781 ccgacgagtt atggccgggg cctcatgcct gacaccttca tcgacttcaa gaagcagcgc
      841 ttccgttggg cctatggggc gatgcagatc ctcaagcgtc acgcgcgcgc ttttttcatg
      901 gaaggaggtt cgctgtcacc gggccagcgc taccatttca tcgctggctg gttaccgtgg
      961 gtggctgacg gatgcaacat gctgttcaat gtggccgcgc tcggctggtc ggcggccatg
     1021 gtgctggacc cccaccggat cgatccgccc ctggtgatgt actcggtgct gccgctctcg
     1081 ctgttcacct tcaagctggc gaagctggtg cacttgtatc gtgtgcgcgt cggcgcgaac
     1141 ttccgtcaga cgatcgcggc cgctatcgcg ggactggcgc tcacccacac catcggtatg
     1201 gccgtggtga aaggactcgt gacgggcagc gagccgttct tccgcacacc caaaaccggc
     1261 caaacagcgc gttgggttca ggcgctcgtc gccgcgcgtg gcgagacatt catgatgctc
     1321 gggctatggc tctccgcctg ggccgtggcc cattctccgc aacagcatgg ccccgatcgg
     1381 ctagcctgga tcgtggtgct gttgatccag tcggtaccct acgtgtcgag tttgttggct
     1441 tcactggcga gtgccttccc actgccggcg cggctgttgg gaaccgggta tcgcccgacc
     1501 agcgcggctt cgcgaccggg gccggggatg ccaagcggta cgcctgccaa cgccgcgggc
     1561 gtccagaacg cggtgggcga gagcggtcgg tctggaccgg ccgatcacgg ggcgcccgag
     1621 gctacgaacg ggccggcgct tttgtaggcg cgcttcgatc cggcgctggc tatcgcaagg
     1681 ttcaggctcg cttcgcgcgc tcgcaagcag cagcggtgtg gcagaggccc tcagagtggc
     1741 atcgctgcgc gcgctttgcg cgctcacaag cggcagcggt gtgtgagagg ccgtgcgagc
     1801 ggtaaccgcc gcggccgtcc gcggcccatc cgggcatcgc ggacatctac gcgccatcgg
     1861 cctcatgctg atcgccgtag cggctttctc cggtatggac tctgtgctga agctcttcgc
     1921 cgctcactac cctcccatgg agatcgcgtt cctgcgcgga ctggcgtcac tgccgttcat
     1981 ggtgctcccc gcgctcttga agggccgcta tcgagacttg aaaccaggcc gcttaggcat
     2041 gcacctgctg cggggcgtgc tgatggtggt gatgctgtgg tgcttcatct atgccgttcg
     2101 caccctctcg ttggctaacg cgtactctat ctatctcgcc gagccgttga tcgtaaccgc
     2161 gctcgcggta ccgttgctcg gcgatcgggt cggatggcgc aactgggtcg ccatctgcgt
     2221 cggattgatc ggagttgtcg tcatcttgcg gccgtcggcc tccggattcg tgaccctcgg
     2281 ctcgctcgcg gcgctcgtgt cggcgactgc ctacgctctt agcgtgatcg ccctgcgggt
     2341 gatcacccgc accgatacaa cgacgagtgt ggtcgtttgg accgtgggac
//
LOCUS       DASRHN010000198        13273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1389849, whole genome shotgun sequence.
ACCESSION   DASRHN010000198 DASRHN010000000
VERSION     DASRHN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389849"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1327
                     /locus_tag="VFB37_07680"
     CDS             143..1327
                     /locus_tag="VFB37_07680"
                     /inference="COORDINATES: protein motif:HMM:NF006097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HZO22372.1"
                     /translation="MSPKPGRSTTAVWGGETEHEPYEGATQVPVVHSVSFGYRDIEAW
                     QAVAQERAPGHIYSRNTNPTVRAFEEKVRDLEGAESATSFASGMAAISATFFALLRPG
                     ERVVSIKDSYGGTNKLFTEFLPPFGIEVCLCDTSDFERIEAEVRRGCRLLYLETPTNP
                     TIKIVDIGRLAQAAHAAGALVAVDNTFATPINQNPLQCGADLVLHSATKYLGGHADAL
                     GGAACGASELVRKLYHFREINGAALAPMDAYNLLRGMKTLALRVERQNDNALSIARWL
                     QSQRVIEQVNYPGLESHLHHDVARRQMRGFGGMLSFSVRGGLEAIKQFLPRLRYAHRA
                     ANLGCVETVVGPPLVTSHVECTPEERAAAGIPEGLVRYSTGIEDVSDLIEDLEQALAC
                     IA"
     gene            1514..1870
                     /locus_tag="VFB37_07685"
     CDS             1514..1870
                     /locus_tag="VFB37_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HZO22373.1"
                     /translation="MPDLTKSSRYTWRGAAVAVLVGGCTLAGSSMAAEPQVIKIQLQY
                     SPAEVGSADGAARLYRRIQFAARQACGEADSREVVRYFLAKKCFERAVDDAVAKVDAP
                     ALTAVHRNHPLRTEAG"
     gene            1931..2566
                     /locus_tag="VFB37_07690"
     CDS             1931..2566
                     /locus_tag="VFB37_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZO22374.1"
                     /translation="MDASVLVPEGAVMITLFHRPKSRSTRFIFLLEELEAPYEIRAVS
                     TRNRDGTGEADPANPHPHGKVPVISDEGEIVFESPAIALYLTDRFPKNRLGPLAGEPG
                     RGRYLSWLSYYTGVLEPAFMSKFMNMPVPRGTAGWVAVEEAMPAVIRILSAGPYMLGE
                     RFSAVDVLYGTTFAMFAQGPLMAKSAVVEEYVQRIVSRPAFARAQARENAG"
     gene            2599..3012
                     /locus_tag="VFB37_07695"
     CDS             2599..3012
                     /locus_tag="VFB37_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZO22375.1"
                     /translation="MMLDPESSTGGCLCGGVRYVVRGALRDVIACHCSQCRRTSGHFV
                     AATSTPLEQFSLTESETLRWYLSSDRAKRGFCSRCGGNLFWQPLDESPARISITAGTL
                     DPPTRLRMSEHIFVADKSDYYNIEDGLPQSPRWPE"
     gene            complement(3019..4677)
                     /locus_tag="VFB37_07700"
     CDS             complement(3019..4677)
                     /locus_tag="VFB37_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22376.1"
                     /translation="MVGHHDALAIFAGRPRAGVILTLLMLAGCPAVQAAQALPPDAEL
                     ERDHAVIGVITIDNQNIFNLADPHENNWVFRLANRLHIRTRPAVIRSQLLFKTGDRYS
                     HRLLEESERVLRSVSYLYDASIRPVGYHDGRVDVLVSTRDVWTLNPGFNYSRSGGSNS
                     TGGQLEELNVLGSGVAVRAQRTSDVDRVINSYQVSDQHAFGTWTAVTGTYENNSDGDM
                     AELTVNRPFYALDTRQAGGLDVERWTRVDPIYDRGVIVDQFHEDLRATSANYGWSQGL
                     QNGWVQRWSVGTTLDQHEFAPTRLWSGPILLPQDRKLVYPWLDFSLVQDDFAKLTNRN
                     QIERTEDFYLGTLLDVRIGWSDQLWGADRKALVFSSSAARGFEFEGGSMLLLSENLSG
                     RLEGTTLRNGILGGSAKFYLVESPRALFYSAVQGTAGHNLDLDNQILLGGDTGLRGYP
                     LRFQTGTASALMTLEQRYFTDWFPFRLWRVGGAVFFDAGRTWGREPLAQPSLGLLEDV
                     GLGLRFGNARSGLGNVTHVDLAMPLERSAGVRGLQFLVQTQQSF"
     gene            4872..5801
                     /locus_tag="VFB37_07705"
     CDS             4872..5801
                     /locus_tag="VFB37_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZO22377.1"
                     /translation="MDQPTAVPLRIGTWRFDPASGQISRAGEVARLEARSLRLLLYLA
                     ERAGETLSIEELLDQVWAGVVVTPDSVYQAIASLRRLLGDDARQPAYIVTVPRLGYRM
                     VAPVSPWLDEPAALIQQAAAGPGVAASASAVNTGKRHRMPVLLTAAGLVLCLALAAAL
                     FYGSKVNGPHILAGTAPAKSVAVLPFLDLTSEQMEEEYFADGMTEELIDKLSALPGMR
                     VPSPAASFYFKGKKVPVAEIARSLGVLYVLSGSVRESASMLRIAVRLTRADDGYVIWS
                     STYDRPAADKVKVQEDIAREVAKALGGWIKQAA"
     gene            complement(5839..6483)
                     /locus_tag="VFB37_07710"
     CDS             complement(5839..6483)
                     /locus_tag="VFB37_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZO22378.1"
                     /translation="MLRRIALAPCLLLVSFCLHATTYTLEPDYTQGVFRWNHLGFSSP
                     AAQFSQGTGTLEFDPADPARSSVTVTIPLSTLYTGVPALDEDFRSKDFFDTAQFPSAT
                     FTSARVEKGATRDRFKLIGELNLHGVTKPVTLEVTVVKVGTNPRTSLATVGFDAVTTL
                     RRSDFGLGKYVPQVSDEIQMRIIVQAVEATSYAAYLKERAAAAAAKAKDAAPKQ"
     gene            6664..8592
                     /locus_tag="VFB37_07715"
     CDS             6664..8592
                     /locus_tag="VFB37_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZO22379.1"
                     /translation="MSGRIGRRDFLSVSLLTAAASALRAQAVTDRPSSSARAAAPGLR
                     VRPFDLHSVRLRPGAALDALMTNRRYLMGLDPDRLLHTFRITAGLPSSAEPLGGWEAP
                     DNELRGHYTGHYLSACALMAAHTGDPAVRDRGTLLARELAKCQAALRTGYLSAFPEEF
                     FDRLRAGQRVWAPFYTLHKIMAGLLDTHRLSGDAPALDTLLGMARWTEGWVAPLDEQQ
                     MARVLEREYGGMNEVLYNLADLTGEARWRELAHRFDKKRTFAPLAAGRDELQGLHANT
                     TIPQIIGAARGFELTGSEPLHAVADNFWHAVAQHRTYCTGGTSNGESWNGPPGKLVHE
                     LSGYTEESCVTYNMQKLTRLVFGWTADPALADYYERAWYNGILGVQHPADGDKLYYLP
                     LQSGYWKLFGTPLHDFWCCTGSMSESFAKLGDSIYFHDAAGVYVNLFVPSELNWAERG
                     VRLVLDTRLPEADTVRLSVRTARPSRFALHLRVPYWTVGGSAQLNGRTLPRFAAPGSY
                     LVLNRTWHDGDVLSVRLPMRLHAAPMPDDATVLAVMYGPLVLAGRLGHEGLGAPTLRA
                     EPTRPRKVPEYPLEPVAAPTIRARSADPATWLETVPGEPLQFRTVGQERVITLEPLNQ
                     VFDERFAVYWKVLPARAA"
     gene            8723..8989
                     /locus_tag="VFB37_07720"
     CDS             8723..8989
                     /locus_tag="VFB37_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HZO22380.1"
                     /translation="MATGWAGEGAVQDQIDATVKDGISRAQSRLPQGPSLTHCALCGN
                     EIPEARRVAVHGVRLCLPCQEAEDRRAEHFSGYNRRGSKDSQLR"
     gene            complement(9019..11169)
                     /locus_tag="VFB37_07725"
     CDS             complement(9019..11169)
                     /locus_tag="VFB37_07725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO22381.1"
                     /translation="MTSLKKSQFLVTIAACFIGPPVALAQAPAARTPITHEKLWMMKR
                     VGSPVVSPDGKWVVFSVLEPSYEQDKEASDLWITPADGSGPARRLTNTRAPENGAAWS
                     PDSRSIAFTTKREGDEAEQVYILDLAVGGEARRLTNISTGATQPQWRPDGKAILFESF
                     VYPNALDDEANRKIATERKARKYNVRIYEHFPIRHWNQWLDDRQPTVIVQGLESGSSA
                     RDILSPTALAHQPGFSGVAGETAISLAPLWSPDGQEIVFTATTERWNAAFAHVGYRMY
                     RMPAAGGSEPRAITTASGQYAEAVFAPDGRSLFFKYAPEDNEVYHLERVERLAWPALG
                     EPTLVTRDFDREVAGYAPTADSRVLYLLTPESGHENLYRAAAAGGRPTLVIGPQVGGY
                     TALAIARKSSRPVLVASYGSSVNPLEIVRIDAAAGRHTNLTNIDTAAAAAIDWQPPEH
                     FTFTSSRGRELHNMIVRPPAFDATKKYPLLVLIHGGAASSNPDQIGLRWNYHLLGSPG
                     YVVLMTDYTGSTGYGEKFTQAIKLDPLKTPGEDINEAVDAALKRYPFIDGTRMCAAGA
                     SYGGHLANWIEATTSRYKCIVSHAGEVDLTTQWGESDSIYDRELTDNGPPWGGSPIWR
                     EQSPLTYAGNWKTPMLLSIGERDFRVPIGNTLENWSALQRMRVPSRLLVWPDAWHWIM
                     KPEDSRRFYEEVHRWLAIYLKGERPAANGRDERQ"
     gene            complement(11275..11622)
                     /locus_tag="VFB37_07730"
     CDS             complement(11275..11622)
                     /locus_tag="VFB37_07730"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZO22382.1"
                     /translation="MDPTRHQRRPQEILRTFVALACAVMVLCGCAAYRKCGLEGCAGD
                     AQITSAVQALLSQHADLGPPNLIRVQTIDGVVYLTGEVNTDLVRETAESVARQAQGAK
                     RVVNSIAITYSGH"
     gene            11773..>13273
                     /locus_tag="VFB37_07735"
     CDS             11773..>13273
                     /locus_tag="VFB37_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO22383.1"
                     /translation="MSRTTKAFLAGSLAALLQSTAVAANPSLMNADQVAALVTEGTIA
                     GIQAAVLAHRASCAAIVSAYLDRIAHLDQRTGLNAITALNPDALRLARQLDAQLQGSP
                     AAHPGALFCVPLLVKDNIDTRGLPTTGGSIALAHNLPTQDAFIVRKLQTAGAIVLAKT
                     NMAEWAFSPRDSVSSSHGVTGNAYDRTRTPAGSSGGTAAAIAASLALVGLGTDTGNSV
                     RGPSGYAALVGMRPTLGLVSRGGIIPLELDRDTAGPITRTVEDNARLLSVMAGSDPED
                     SLTADADARRAQDYTRFLKPDALRGARIGVVRQLAAPDETNPQVMASFNQAIESLRQA
                     GAIIVDPLTIPNLSRHLESGYYCPRFAFDVNTYLNQPIHPRAPRDIQQIYASGAYSPH
                     SRSDFERFLKGSATDPALSSPPCPLYLAHPGRRAMLHDVESAMSAAKLSALIYPTWLI
                     LPPTLDQAVSAYRGDNSQLLSPPTGMPAITLPSGFANGLPMGLQLLGRRF"
BASE COUNT         2287 a         4322 c         4300 g         2364 t
ORIGIN      
        1 accggggggg gtgacatgga tcagcagcag tcccagggat tggggaatgt gagcgtccca
       61 gcttcgggac ccgctggacc gagatagtcc tgagtcaacc ggatcggatg cgcgggccga
      121 ccggcccgtc gcgggggaat ccatgagtcc gaaaccgggc agatccacga ccgcggtctg
      181 gggcggggaa accgagcacg agccctacga aggcgccacc caggtgcctg tcgtacacag
      241 cgtctccttc gggtatcgcg acatcgaggc ctggcaggcc gtcgcccagg agcgcgctcc
      301 cggacacatc tattcgcgca ataccaaccc aacggtacgc gccttcgagg agaaggtccg
      361 ggacctggag ggggcggaat cggccaccag cttcgcttcc ggcatggcgg ctatcagcgc
      421 tacgttcttc gcgctgctgc gccccggtga gcgcgtcgtc tccatcaagg acagttatgg
      481 gggcacgaac aagctgttca ccgaattcct gcccccgttc ggcatcgagg tctgcctgtg
      541 cgacacgagc gatttcgagc gcatcgaggc ggaagttcgt cgcggctgcc ggcttctgta
      601 tctcgagacc cccaccaatc caaccatcaa gatcgtcgac atcgggcgcc tggcgcaggc
      661 tgcgcatgct gccggcgcgc tcgtcgcggt ggacaacacc ttcgctacac ccatcaacca
      721 gaatccgctg caatgtggcg ccgatctggt gttgcatagt gcgaccaagt acctcggcgg
      781 tcatgcggat gcgctcggcg gggccgcatg tggcgcatca gagctggtgc gcaagctcta
      841 tcacttccgt gaaatcaacg gtgcggcgct cgcgccgatg gatgcctaca acctgttgcg
      901 cggaatgaag acactggccc tgcgtgtcga acgacagaat gacaatgccc tgagcatcgc
      961 gcgctggctg cagagccagc gcgtcatcga gcaggtgaac tatcccggtc tcgagtctca
     1021 tctgcatcat gatgtcgcca gaaggcagat gcgcggcttc ggcggcatgc tgagcttcag
     1081 cgtgcgcggg ggcctcgagg ccatcaagca gttcctcccg cgcctgcgct acgcccatcg
     1141 tgcggccaat ctgggatgcg tcgaaacggt tgtcggtccg ccgctggtga cgagtcacgt
     1201 cgaatgcaca ccggaggagc gggcggcagc cggaattccg gagggcctgg tgcgttattc
     1261 gacgggaatc gaggacgtgt cggacctcat agaggatctc gagcaggccc tggcctgcat
     1321 cgcctgagat cagccgcaca ggcgtgcggc atcgatctgc gcgcattgcg gcgggaacgc
     1381 agcggattgt ctttgcaatc gggcgccctg tcacagcccg acgaccaccc gtcacatcct
     1441 gcgccagctt cgcagctgca ggcctatcgt cgtggcgctg ttgcaggcag cagtcacacg
     1501 atcgggaggc gttatgccgg atctcacgaa gagctctcgt tacacctggc gtggagcggc
     1561 cgtcgctgtg ctggtcgggg gctgcacgct cgcgggcagc tcgatggcgg cggagccgca
     1621 ggtgatcaaa atccagctgc agtactcgcc tgcggaagtc ggctcggccg atggggcggc
     1681 gcggctttac cgccgcatcc aatttgccgc gcgccaggcc tgcggggagg cggacagccg
     1741 cgaggtcgtg cgctacttcc tcgcaaagaa gtgcttcgag cgtgccgtgg atgatgcggt
     1801 tgcaaaggtc gatgcgccgg ctctcaccgc agttcatcgc aaccacccct tgcgcacgga
     1861 agccggctga gcacgcccgg atctgcgcgg gcgctcgccg ttgcggcagt gcccgccccc
     1921 tcgtggcaca atggacgcct ccgtcctcgt gcccgagggt gccgtcatga tcacgctgtt
     1981 tcatcgcccg aaatcgcgat cgacgcgttt catattcctg ttggaggaac tcgaggcgcc
     2041 ctacgaaatc cgggcggtga gcacgcgcaa ccgcgacggt acgggagaag ccgatcctgc
     2101 caacccgcac ccgcatggca aggtgccggt gatctccgat gaaggggaaa tcgtcttcga
     2161 atcgcccgcc atagcgctct atctcaccga ccggttcccg aaaaaccggc tcggcccgct
     2221 cgcaggcgag cccggacggg gccgctatct atcctggctg tcgtactaca cgggcgtcct
     2281 cgagccggcg ttcatgtcca agttcatgaa catgcccgta ccgcgtggca cggcgggctg
     2341 ggtcgctgtc gaggaggcca tgcctgcggt catccgcata ctctcggcgg gtccgtacat
     2401 gctcggcgag cgcttcagcg ccgtggacgt cctttacggc acgacgtttg cgatgtttgc
     2461 acagggcccg ctgatggcca agtctgcagt cgtggaggag tacgtgcagc ggatcgtgtc
     2521 caggcccgcg ttcgcacgcg cccaggcgcg cgagaacgcg ggctgatcga tcctcggaaa
     2581 gcgcagccgg aggcaagcat gatgttggat cccgagtcga gcactggagg ctgtctttgc
     2641 ggcggcgtcc gctacgtcgt gcgaggcgca ctgcgcgacg tgatcgcgtg tcattgctct
     2701 caatgccggc gcacgagcgg tcacttcgtg gccgcgacga gcacgccgct cgagcaattc
     2761 agcctgaccg agtccgagac cctgcgctgg tatctgtcct ccgatcgcgc gaagcgcggc
     2821 ttctgcagcc gttgcggcgg aaatctgttc tggcagcccc tcgatgagtc gccggcccgg
     2881 atctcgatca ccgcgggcac gctggacccg cccactcgct tgcggatgag cgagcacatc
     2941 ttcgtcgccg ataaatcgga ctactacaac atcgaggacg gcctgccgca gtccccgcgc
     3001 tggccggagt aggctcgctc agaaactctg ctgcgtctgc accaggaact gcaaaccgcg
     3061 cacgccggcg ctgcgctcga gtggcatcgc gagatcgaca tgggtgacgt tgccgagacc
     3121 ggaacgggcg ttgccgaagc gcagccccag gccgacatcc tcgagcagcc cgaggctcgg
     3181 ctgcgccagc ggttccctgc cccaggtgcg accggcatcg aagaacaccg caccgcccac
     3241 ccgccacagc cggaagggaa accagtcggt gaagtagcgc tgctccagcg tcatcagcgc
     3301 actcgccgtc cccgtctgaa agcgcagcgg atagccgcgc agcccggtat cgccgccgag
     3361 cagaatctgg ttgtccaggt ccagattgtg gcccgccgtc ccctgcaccg ccgaatagaa
     3421 tagcgcccga ggagattcca ccaggtagaa cttggcactg ccgcccagga tgccgttgcg
     3481 tagcgtggtc ccttccagcc ggccggacag gttctcggac agcaacagca tcgatccgcc
     3541 ttcgaattca aagccccgcg cggccgagct cgaaaacacc agcgccttgc ggtccgcgcc
     3601 ccagagctga tccgaccagc cgatgcgtac atcgagcagc gtcccgaggt aaaaatcctc
     3661 ggttcgctcg atctggttgc gattcgtcag cttcgcgaaa tcgtcctgaa ccagcgagaa
     3721 atcgagccag ggatagacga gctttcgatc ctggggtagc agtatcggcc cgctccagag
     3781 ccgggtgggc gcaaactcgt gctgatcgag cgtggtcccg acgctccagc gctgcaccca
     3841 gccgttctgc agaccctgtg accaaccata gttggcggag gtcgcgcgca gatcctcgtg
     3901 gaattgatcg acgatcacgc cgcggtcgta gatcggatcc acgcgcgtcc agcgctccac
     3961 atccaggccg cccgcctgac gggtatcgag ggcatagaac ggacggttca cagtcagttc
     4021 cgccatgtcg ccgtcgctgt tgttctcgta cgtcccggtc accgcggtcc aggtgccgaa
     4081 ggcgtgctga tccgacacct ggtacgagtt gatgactcga tcgacatcgc tcgtgcgttg
     4141 ggcgcgcacc gccacgccgc tgccgagcac attgagctcc tcgagctgcc ctccggtgga
     4201 gttgctgcct ccgctgcggc tgtaattgaa gcccggattg agcgtccaca catcgcgcgt
     4261 gctcaccaga acatccacgc ggccatcgtg atagcccacc ggccgaatgg aggcatcgta
     4321 cagataggag accgatcgca ggacccgttc ggactcctcc agcagccggt gtgagtaccg
     4381 gtcgccggtc ttgaacagga gctgtgagcg gatgacggct ggacgggtgc gaatgtgaag
     4441 gcggttggcc agcctgaaca cccagttgtt ctcgtgcgga tcggcgagat tgaagatgtt
     4501 ctggttgtcg atcgtgatca caccgatcac cgcatgatca cgctcgagct cggcatccgg
     4561 gggaagcgcc tgggcagcct gcacggcggg gcagccggcc aacatcagta gtgtgaggat
     4621 cacacctgcg cgcggccggc cagcgaagat tgcgagagca tcgtggtgcc caaccatggc
     4681 gagcgagttt cgcagtggtc gacgtccgct gcaatgcgac gctccacgct tgaggcggcg
     4741 ggccaaagcg cggcttgtac ccgtgccagc aggccagcgg ggcaaccccg cagcgcccgc
     4801 gtggctcaac gcagccctct gctatactgc gccagcccag aatccgcccg actgaacggg
     4861 ttgccggctg catggatcaa cctactgcag tacccctgcg catcggtacc tggcgctttg
     4921 atccggcgag cggtcagatt tcccgagcag gcgaagtcgc ccggctcgag gcccgctccc
     4981 ttcgtttgtt gctctacctg gccgaacgag cgggtgagac cctcagcata gaagagctgc
     5041 tcgaccaggt ttgggcaggc gtcgtggtca ctcccgactc ggtctatcag gcgatcgctt
     5101 cgttgcgccg cctcctgggc gatgatgcca ggcaacctgc ctacatcgtg accgtgccgc
     5161 ggttgggata tcgaatggtg gcaccggtga gtccctggct ggatgagcct gctgcgctga
     5221 tccagcaggc ggcggcgggc cctggcgtcg ctgcctccgc ctcagcggtc aacaccggca
     5281 agcgtcaccg catgccggtg ttgttgaccg cagcggggct ggtgctgtgt cttgcgctcg
     5341 cagcggcctt gttctacgga agcaaggtaa atggccctca catcctcgcc ggcactgcgc
     5401 ccgcgaaatc cgtcgctgtg ctgccatttc tcgatctgac cagcgagcag atggaggagg
     5461 agtatttcgc cgacggcatg accgaggagt tgatcgacaa gttgagcgcg ctgccgggca
     5521 tgcgggtgcc ctcccccgcg gcctccttct atttcaaggg caagaaggta ccggtagccg
     5581 agatcgccag atcactgggc gtgctctacg tgctttcagg aagcgtacgc gaatccgcca
     5641 gcatgctgcg gatcgccgtg cgactgacgc gcgccgacga cggctacgtg atctggtcct
     5701 cgacctatga ccggccagcc gccgacaagg tgaaagtcca ggaggacatc gcgagggagg
     5761 tcgccaaggc gcttggcggg tggatcaaac aggccgccta gctcctgcgg cgtccggtgc
     5821 gctcggcgat cggaaccatc attgtttcgg cgctgcatcc ttcgccttcg cggcggccgc
     5881 cgctgcccgc tccttcaaat aggccgcgta cgaagtcgct tccacggcct gaacgatgat
     5941 gcgcatctgg atctcgtcac tgacctgggg cacgtacttg cccaatccga agtcggagcg
     6001 tctgagcgtg gtcacggcat cgaaacccac cgtcgccagg ctggtcctcg ggttcgtgcc
     6061 gaccttgacc acggtgactt cgagcgtgac tggcttcgtc acgccatgca ggttgagctc
     6121 gccgatgagc ttgaatcggt cccgcgtcgc ccccttctcg acccgcgcac tggtgaaagt
     6181 cgcgctcgga aactgagccg tgtcgaagaa atccttcgaa cggaaatcct catccagtgc
     6241 aggcacaccg gtgtagaggg tactcagtgg aatggtgacg gtgacggacg accgggcggg
     6301 atccgccggg tcgaattcca gcgttcccgt cccctgcgag aattgcgccg cgggactcga
     6361 gaagcccagg tggttccagc gaaagactcc ctgcgtgtag tccggttcga gggtataggt
     6421 cgtcgcgtga aggcagaagg atacgagcag caggcaagga gcaagagcaa tgcggcgaag
     6481 catcgtagga ccccatgatg gcgtggtgag tgggcagcac gatgtcgcga cccacgctgg
     6541 aagtcctgaa gcgcgcctga aaagggctga atacttttga aaacgcgcgc tgggaaggcg
     6601 ctggactggc tccgcatgaa ctgggtatcg tggccatcac ggtcagcttt gtggatgagt
     6661 gggatgtccg gtcggatcgg tcggcgtgat ttcctgtcag tgtcgttgct cacggcggcc
     6721 gcatccgccc tgcgggccca agcagtcacc gaccgcccga gcagctcagc tcgcgctgcc
     6781 gcaccaggct tgcgcgtccg tcccttcgat ctgcattcgg tgcgtttgcg gcccggtgcg
     6841 gcgctcgatg cactcatgac caatcggcgt tacctgatgg gtctcgaccc cgatcggctc
     6901 ctgcacacgt ttcgtatcac ggccgggctg ccgagctcgg ctgagccact cggcggctgg
     6961 gaggcgcctg ataacgagct gcgcggtcac tacaccggac actacctgtc ggcttgcgcc
     7021 ctgatggcgg cccacaccgg ggatccggcg gtcagggatc gcggcactct cctcgccaga
     7081 gagctggcga aatgtcaggc tgccctgcgc accggctacc tgagcgcatt tcccgaggag
     7141 tttttcgatc ggctgcgcgc gggccagcgg gtctgggcgc ccttttacac gctgcacaag
     7201 atcatggccg ggttgctcga tacccatagg ctcagcggcg atgctccggc gctcgacact
     7261 ctcttgggca tggcgcgctg gacggagggc tgggtcgcgc cgctcgatga gcagcagatg
     7321 gctcgggtgc tcgagcgcga gtacggcggc atgaacgagg tgctctacaa cctggcggat
     7381 ctcacaggcg aggcacgctg gcgcgagctg gcccatcggt tcgacaagaa gcgcacgttt
     7441 gcccctcttg cggccggccg cgacgagctg cagggactgc atgccaacac gacgatcccg
     7501 cagatcatcg gggcggcgcg cggtttcgag ctcaccgggt ccgaaccgct gcatgcggtg
     7561 gcggacaatt tctggcacgc cgtggcgcag catcgaacct actgcaccgg cggtaccagc
     7621 aatggggaat cttggaacgg cccgcccgga aagctcgtgc acgagctttc cgggtacacg
     7681 gaagagtcgt gcgtcacgta caacatgcag aaactaacgc ggctggtttt cggctggaca
     7741 gccgacccgg cgctggccga ctactacgag cgcgcctggt ataacggcat tctcggggtc
     7801 cagcatcccg ccgacgggga caagctctac tacctgccgc tgcagagcgg ttactggaag
     7861 ctcttcggaa cgccgctgca cgatttctgg tgctgcacgg gaagcatgtc ggagtcattc
     7921 gccaagctgg gcgacagtat ctattttcac gacgccgcgg gcgtgtatgt gaatctcttc
     7981 gtcccctcgg agctgaactg ggccgagcga ggagtccggc tggtgttgga tacgcgcctc
     8041 cccgaggcag acaccgtgcg attgtcggtg cggaccgcca ggccctcgag gttcgccctg
     8101 cacctgcgtg tcccctactg gacggtcggc ggctctgcac agctcaacgg taggacgttg
     8161 ccgcgcttcg ccgcgccggg cagctatctc gttctgaatc ggacctggca cgacggcgac
     8221 gttctttccg tgcgcttgcc gatgcggctg cacgccgctc cgatgcccga cgatgccacc
     8281 gttctggcgg tcatgtatgg accgctcgtg cttgcggggc ggctcggcca cgaaggtctg
     8341 ggcgcgccca ctttgcgcgc cgagccgacc cgaccgcgaa aagtgccgga atatcctctc
     8401 gagccggtgg ccgcgccgac catccgcgcg cgctctgcgg atccggccac ctggctggaa
     8461 actgtaccgg gggagccctt gcaattccgc accgtgggac aagagcgggt gatcacgctc
     8521 gagcccttga accaggtgtt cgatgagcgc ttcgcggtgt actggaaggt gctccccgcg
     8581 cgcgccgcct gaggcggccc ggcaattggg cgcgcagcgg cattatgtct gggaagacgg
     8641 ggcgtcggcg ggggcttggg caccatcttg aaatagcgtg cgaccagcaa catagtgttg
     8701 gggaggactt tgcggaggca acatggcgac gggctgggca ggagaggggg cggtacagga
     8761 tcagatcgac gcgaccgtca aggacgggat cagtcgcgct caaagccgcc tgccgcaggg
     8821 tccgtcgctt actcactgcg ccctgtgcgg caacgagatt ccagaggcac gccgggtggc
     8881 cgtgcatggt gtgcggctgt gcctgccctg ccaggaagcc gaggaccgca gggccgagca
     8941 cttcagcggc tataaccggc gcggcagcaa ggatagccag cttcgctgag cgcgcatcat
     9001 cgtaagcttc gcggcagttt actggcgctc atcgcgccca tttgcggcag gcctctcccc
     9061 cttgagatag atcgcgagcc agcgatgcac ttcctcgtag aagcgacggc tgtcctcagg
     9121 cttcatgatc cagtgccagg catcgggcca cacgagcagg cggctcggta ctcgcatgcg
     9181 ctgtaaggcg ctccagttct cgagcgtgtt gccgatgggc acgcgaaagt cgcgttcgcc
     9241 gatgctcagc agcatcggcg tcttccagtt gcccgcatag gtgagcgggc tctgctcgcg
     9301 ccagatgggg ctgccgcccc acggcggccc attgtcggtg agctcccggt catagatcga
     9361 atcgctctcg ccccattggg tggtgagatc cacttcgccg gcgtgactga cgatgcactt
     9421 gtaacggctg gtcgtggctt cgatccagtt ggccaggtgt cctccgtaac tcgcccctgc
     9481 ggcgcacata cgcgtgccgt cgatgaacgg atagcgcttg agtgcggcat cgacggcctc
     9541 gttgatgtct tccccggggg tcttcagcgg gtcgagcttg atggcctggg tgaacttctc
     9601 gccgtagccg gtcgagccgg tgtagtcggt catcaggacg acatatcccg gcgaccccaa
     9661 cagatgatag ttccagcgca atccgatctg gtcgggattg ctgctggccg caccgccgtg
     9721 aatgagcacc agcagcgggt atttcttcgt cgcatcgaag gcgggcggcc ggacgatcat
     9781 gttgtggagc tcgcgcccgc gggagctcgt gaaggtaaag tgctcgggcg gctgccaatc
     9841 gatggcggct gcggccgccg tgtcgatgtt ggtgaggttg gtgtgccggc ccgccgcggc
     9901 atcgatgcgt acgatctcga gcgggttgac cgagctgccg taactggcaa ccaacacagg
     9961 ccgggagctc ttgcgcgcaa tggcgagcgc cgtgtacccg ccaacctgcg ggccgatgac
    10021 cagtgtcgga cggccgccgg cggcggcggc gcgatacagg ttctcgtgcc cgctctccgg
    10081 cgtgagcaga tacagaacgc ggctgtccgc agtcggagcg tatccggcga cctcacgatc
    10141 gaagtcgcgc gtcaccaggg tcggctcgcc gagcgctggc caggccagac gctccacccg
    10201 ctcgaggtga taaacctcat tgtcctcggg cgcatacttg aagaagagcg aacgaccatc
    10261 cggtgcgaag accgcctcgg cgtattggcc tgatgccgtt gtgatcgcac gcggctcgga
    10321 gcccccggcg gcaggcatcc ggtacatccg gtaacccacg tgcgcgaagg cggcattcca
    10381 gcgctccgtg gtggccgtga acacgatttc ctgcccatca ggactccaca gcggtgcgag
    10441 cgatatggcc gtctcgcccg ccactccgga gaatccgggc tggtgcgcca aggcggtcgg
    10501 cgaaaggatg tcgcgggcgc tagatccgct ctccagcccc tgcacgatca cggtcggctg
    10561 acgatcatcc agccactgat tccagtggcg aatggggaaa tgctcgtaga tacgcacgtt
    10621 gtacttgcga gccttacgct cggtggcgat cttcctgttg gcctcatcgt caagcgcatt
    10681 tggatagacg aacgactcga acaggatcgc cttgccgtca ggccgccatt ggggctgggt
    10741 ggcgcccgtg gagatgttgg tcaatctacg cgcctcacct ccgacggcca gatcgagaat
    10801 gtagacctgc tcggcctcgt cgccttcgcg cttggtggtg aacgcgatcg agcggctgtc
    10861 tggagaccac gcggctccgt tctcgggcgc ccgcgtgttg gtgagtcggc gggccgggcc
    10921 gctgccatcg gccggcgtga tccacagatc gctcgcttcc ttgtcctgct cgtaggaggg
    10981 ctcgaggacg gagaacacga cccatttccc gtccggactt accacgggtg aacccacccg
    11041 cttcatcatc cagagcttct catgtgtgat cggcgttctg gccgccggcg cttgcgccaa
    11101 tgccacgggc ggcccaatga aacaggcggc aatcgtgacg agaaactgac ttttcttgag
    11161 acttgtcacg gcaggccctc cccggccaag ggccgcagca tagaggattc cacgaggggc
    11221 ggttaatgtc cgttgtaagg ggctgcggtt agtgtccgct gtaagggggt gcggttagtg
    11281 tccgctgtaa gtgatcgcta tggagttcac gacccgcttc gccccttgcg cctggcgcgc
    11341 gaccgattcc gccgtctcgc gcaccagatc ggtgttgacc tcgcccgtca ggtacacgac
    11401 cccatcgatc gtctgcactc gaatgagatt cggcggccca agatcggcat gctggctcag
    11461 caacgcctgc accgcggagg tgatctgcgc atccccggca cacccttcca ggccacattt
    11521 gcggtatgcc gcgcagccgc agagcaccat gaccgcgcac gcgagcgcga cgaaggtcct
    11581 gaggatctcc tgcggccgcc tctgatgcct tgtcggatcc atacgccgat ctccgggacg
    11641 tgaccgtgtc gctcggggcg cccgcaggat gccggccggc aacgcggtat ggcgggactc
    11701 ctcacatcag cacgccgcgt tccataatat ctgcgcaacg tggatccagc atgaatgcac
    11761 cgggggagca cgatgagtcg aacaacaaaa gcgttcctgg cgggcagcct cgcggcgctc
    11821 ctgcagagca ccgcggtcgc cgcgaacccc tccctcatga atgccgatca ggtggccgcc
    11881 ctggtcaccg aaggcaccat cgccgggatc caggctgcgg ttctggcgca tcgcgccagc
    11941 tgtgccgcaa tcgtcagcgc ctacctggat cggatcgccc acctcgatca gcggacgggc
    12001 ctcaatgcca tcaccgcatt gaatccggat gcattgcgtc tggctcggca gctcgatgct
    12061 caactgcagg gctcgcctgc tgctcatccg ggagcgctgt tctgcgttcc gctgctggtc
    12121 aaggacaaca tcgatacccg gggcttgccc acgacgggcg gatccatcgc cctcgcccat
    12181 aacctgccta cccaggacgc cttcatcgtc cgcaagctcc agacagccgg ggccatcgtt
    12241 ctggccaaaa ccaacatggc ggagtgggcc ttcagtccgc gcgacagcgt gagctcctcg
    12301 catggagtca ccggcaacgc ctatgaccgc acccgcaccc ccgcgggctc ctcgggaggg
    12361 accgccgccg caatcgcagc cagcctcgcg ctggtgggtc tcggaacgga taccggcaat
    12421 tcggtgcgcg gaccttccgg ctacgcggct ctggtgggca tgcggcccac cctcggactg
    12481 gtgagccgtg gcggaatcat tccgctggaa ctggatcggg ataccgccgg gccgatcacg
    12541 cgcacggttg aggacaacgc ccgtcttttg agcgtcatgg cgggcagcga cccggaggac
    12601 tcactgaccg ccgatgcgga tgcgcgccgc gcacaggatt acacccgctt cctcaaaccg
    12661 gatgccttgc gcggagcccg gatcggtgtc gtgcgccagt tggctgcacc cgacgaaacg
    12721 aacccacagg tgatggcatc cttcaaccag gcgatcgaga gcctgcgtca ggccggcgcg
    12781 atcatcgtgg acccgctgac cattcccaat ctgagccggc atctggagag cggctactac
    12841 tgcccgcgct tcgcattcga cgtgaatacg tatctgaatc agccgatcca cccccgggcg
    12901 cctcgggata tccagcagat ttacgcatcc ggggcctact cgccgcacag ccgcagtgat
    12961 ttcgagcggt ttctcaaggg atcggcgact gatcccgcct tgagctcgcc gccctgtccg
    13021 ctgtatctcg cgcatccggg ccgacgcgcg atgctgcacg atgtcgaatc cgccatgagc
    13081 gccgcaaagc tcagcgcgct catctacccg acgtggctca ttctgccgcc gacactggac
    13141 caggcggtca gcgcctatcg cggtgacaac tcgcagttgc tgtctccgcc gaccggcatg
    13201 cccgccatca cgctgccatc aggattcgcg aacggcttac ctatgggcct gcagctcctc
    13261 ggccggcgct tcg
//
LOCUS       DASRHN010000199         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_892989, whole genome shotgun sequence.
ACCESSION   DASRHN010000199 DASRHN010000000
VERSION     DASRHN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892989"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..808
                     /locus_tag="VFB37_07740"
     CDS             173..808
                     /locus_tag="VFB37_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HZO22384.1"
                     /translation="MDTRRGTLIIACGALAHEISALQRASGWNALTVQCLPPELHNRP
                     ERIAPAVRAQIRQNRERYRTIFVAYADCGTGGLLDAVLREESVERLPGAHCYEFFAGP
                     RLFAELAEAEPGTFYLTDFLLRHFERLVIRGLGLDEHPELHQQYFRHYRRLVYLAQTR
                     SEQAQQEASRIAQRMGLAFEYRFTGYGELGERLQALLSRQRAAQPENAWPA"
     gene            796..1119
                     /locus_tag="VFB37_07745"
     CDS             796..1119
                     /locus_tag="VFB37_07745"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HZO22385.1"
                     /translation="MASLTIISWRDIPAQVVGRRGRETAKVQLSARFQEAVDRAAMRA
                     GKGSSDAYLADWKRSEPRPCDEDIQAVVTAVAAEIEARYTDEELERLIRAKGLAAGAP
                     AGGPT"
     gene            1133..1591
                     /locus_tag="VFB37_07750"
     CDS             1133..1591
                     /locus_tag="VFB37_07750"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZO22386.1"
                     /translation="MYAVRRWSVRHARGLNAFYRGFEAVLVKLHPLWKRIGYARLERP
                     VAATERLVKGVLFDCRMCGQCVLSSTGMSCPMNCPKNLRNGPCGGVRTDGSCEVRPEM
                     KCVWHEAFAGSARIPGGTRALRMLQPAIDRRLQGRSSWLRVVRERAGDNA"
     gene            1588..2607
                     /locus_tag="VFB37_07755"
     CDS             1588..2607
                     /locus_tag="VFB37_07755"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZO22387.1"
                     /translation="MNFEDEPVPGYPLPILPGHTSPGRLERVLRAGQFAVTSELAPPD
                     SADPEDVYKRARIFDRYVDAINATDGSGANCHMSSLAVCALLTRVGYAPVMQISCRDK
                     NRIAIQGDILGGAAMGVCNLLCLTGDGVQAGDHPQALPVFDLDSISLLETARTLRDEH
                     HFLSGRKITYAPRVLLGAAENPFAPPIAWRAQRLAKKVAAGAQFIQTQYCYDVPLLRS
                     FLRQVEDLGLLEQIFILVGVGPLRSAKTAEWMRTHVPGLHIPDEVVARLAGASEPARE
                     GRKLCIELIQEIRAIRGVSGIHLMAYRQEESVAEIIDRSGVLGGRVPWYPGRDTELNT
                     SRIAS"
     gene            2604..3536
                     /locus_tag="VFB37_07760"
     CDS             2604..3536
                     /locus_tag="VFB37_07760"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZO22388.1"
                     /translation="MTDTVISSATREVVIGFERPFVMIGERINPTGRKILAAEMAAGD
                     FSRVESDARAQIAAGAHMLDVNAGIPLADEPAILAKAVQLVQSITDVPLSIDSSIVAA
                     LQAGLAAYKGKPLVNSVTGEEERLESVLPLVKKYGAAVVAISNDETGISEDPDVRFAV
                     ATKIVHRAADHGIPASDIVVDPLVMPIGAINQAGVQVMHLVRRLRNELKVNTTCGASN
                     VSFGLPAREGVSAAFLTMAIGAGMTSAIMNPLHLEIVKACMAADVMMGHDPDCARWIR
                     RFREAPVMQAGGGEAKPQEGAARGRREGGHRRRV"
     gene            3590..>4887
                     /locus_tag="VFB37_07765"
     CDS             3590..>4887
                     /locus_tag="VFB37_07765"
                     /inference="COORDINATES: protein motif:HMM:NF036965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZO22389.1"
                     /translation="MTDSDALVVFTPSGKRGRFALGTPLLQAARSLGVDVDSVCGGRA
                     LCGRCQVLVMEGDFPKHGVNSRSENLSPLSAAERSYSRRRPLPEGHRLSCASVVRGDL
                     VIDVPPSSQVHRQVVRKAADARVITLDPLVHLHYVEVQQPDMHDPSGDLQRLMNALQT
                     EWNLSGLECDLRVLQNLQSALRKGGWRVTVAVHGGSQIVGIWPGLHERLFGLAVDVGS
                     TTIAAHLCDLESGEVVASSGSMNPQIRFGEDLMSRVSYSMMNPGGAQQMTQVVRSAIN
                     GLAGEVAREANISIQEILEVTFVGNPIMHHLLLGIDPVELGGAPFALTTDGALTMWAT
                     EIDLQLHPNARIYVLPCIAGHVGADAAGMILAERPDLGAELTLLVDVGTNAEIVLGNR
                     DRLLACSSPTGPAFEGAQISCGQRAAPGAIERVRIDPQSL"
BASE COUNT          794 a         1606 c         1630 g          857 t
ORIGIN      
        1 aaggactaca tcgtgctcgt cggcggtgcg cccctgaacg aggaattcgg caaggctgtg
       61 ggcgccgatg catattgccg tgacgcggcg gttgcggtcg agactgcgaa gggtcttctc
      121 gccgcgcgcc gtgctgcacg cgtggccggt tgagcgacat ggacagccgt tcgtggacac
      181 gcgtcgcggc accctgatca tcgcctgcgg agcacttgct cacgagatca gcgccctgca
      241 gcgcgcaagc ggctggaacg cgctgacggt ccagtgtctg ccgcccgaac tgcacaatcg
      301 tcccgagcgc atcgcgcccg ccgtgcgggc gcagatccgc cagaatcgcg aacgctatcg
      361 caccatcttc gtcgcctatg ccgactgtgg cacgggcggc ctgctcgatg cggtgctgcg
      421 cgaggaatcc gtcgaacgct tgccgggcgc gcattgttat gagttcttcg ccggcccacg
      481 cctgttcgca gagctggccg aggcagagcc cggaaccttc tatctgaccg acttcctgct
      541 gcggcatttc gagcggctcg tgatccgcgg cctggggctc gacgagcatc ccgagctgca
      601 tcagcagtat tttcggcatt accgccgact ggtttatctt gcgcaaaccc gctccgagca
      661 ggcccagcag gaggcgagcc ggattgcgca gcgtatgggt cttgccttcg agtaccgctt
      721 caccggctac ggagaactgg gcgagcggct gcaggctctg ctttcgcgcc agcgtgctgc
      781 gcagccggag aacgcatggc cagcctgacc atcatctcct ggcgcgacat ccccgcacag
      841 gtggtgggcc gacgtggtcg cgagaccgcc aaggtgcaac tctcggcgcg ctttcaggag
      901 gcggtggacc gcgccgccat gcgcgcaggc aaaggcagct cggatgccta tctcgcggac
      961 tggaagcgca gtgagccccg gccttgcgac gaagacattc aggctgtggt caccgcggtc
     1021 gctgccgaga tcgaggcacg ttacacggat gaggagctcg agcggttgat ccgcgcgaag
     1081 ggattggccg cgggcgctcc cgcgggcggc cccacgtgat cggagccctc tgatgtatgc
     1141 cgtgcgccgc tggtcggtgc gtcacgctcg cggactcaac gctttctatc gggggttcga
     1201 agccgtactg gtgaagctgc atccgctgtg gaagcgaatc ggctatgccc ggctcgaacg
     1261 gccggtggcc gcgaccgagc gactcgtcaa gggtgtcctg tttgattgcc gcatgtgcgg
     1321 gcagtgcgtg ctgagctcca cggggatgtc ctgcccgatg aactgcccga agaatcttcg
     1381 caatggcccg tgcggcggcg ttcgcaccga cggcagttgc gaagtccgcc cagagatgaa
     1441 atgcgtctgg cacgaggctt ttgcgggaag cgcccgcatc ccgggaggca ctcgcgcctt
     1501 gcggatgctg cagccggcaa tcgaccggcg cctccagggg cgctcttcct ggttgcgtgt
     1561 cgtgcgcgag cgcgcgggag acaacgcgtg aacttcgagg atgagccggt tcccggctac
     1621 ccgttgccga tcctcccggg ccacacttct cccggccggc tggagcgcgt gctgcgcgcc
     1681 gggcagttcg cggtcaccag tgagctggcg cccccggact ctgcggaccc cgaggacgtc
     1741 tacaagcggg cgcggatctt cgatcgctac gtcgatgcca tcaatgcgac cgacggcagc
     1801 ggcgcgaact gccacatgtc gagcctcgct gtgtgcgccc tcctcacccg cgtgggctat
     1861 gcccccgtga tgcagatctc ctgccgggat aagaaccgca tcgccatcca gggtgacatt
     1921 ctgggtgggg cggcgatggg ggtctgcaac ctgttgtgtc tgaccggaga tggggtgcag
     1981 gcgggcgatc acccgcaggc cctcccggtc ttcgatctcg attcgatctc gctgctcgaa
     2041 accgcccgca cgctgcgcga cgagcaccat ttcctcagtg gacgcaagat cacttatgcc
     2101 ccgcgcgtcc tgttgggcgc tgccgagaat ccctttgctc cgccgatcgc ctggcgtgcg
     2161 cagcggctcg ccaagaaggt cgccgcaggg gcgcaattca ttcagaccca gtactgctac
     2221 gacgtccccc tgctgcgcag cttcctgcgc caggtggagg atctcgggct gctcgagcag
     2281 attttcatcc tggtcggagt ggggccgctg cgctcggcca agaccgccga gtggatgcgg
     2341 acccacgtac ccggacttca cattccggac gaggtggtcg ctcgcctggc cggggccagc
     2401 gaaccggcgc gcgaagggcg caagctctgc atcgaactca tccaggagat ccgcgccatc
     2461 cgcggcgtga gcggcatcca cctgatggcg tatcgccagg aagagagtgt cgcagaaatc
     2521 atcgaccgct ccggggtcct cggtggccgc gtgccgtggt acccgggccg agatacagaa
     2581 ctcaatacca gcaggattgc ctcatgaccg acaccgtcat cagctcggcc acccgcgaag
     2641 tcgtcatcgg cttcgagcgc cccttcgtca tgatcggcga gcggatcaac ccgaccgggc
     2701 gcaagatcct ggccgccgag atggccgccg gcgatttcag ccgggtcgaa tcggatgctc
     2761 gcgcgcagat cgccgcaggg gcgcacatgc tggatgtgaa tgcgggtatc ccactggcgg
     2821 acgagccggc gattctcgcc aaagcggtcc agctcgtgca atcgatcacg gacgtgccgc
     2881 tgtcgatcga ctcctcgatc gtggccgcgc tgcaggccgg gctcgcggcg tacaagggca
     2941 agccgctggt caactccgtg acgggcgaag aggaacgcct cgagagcgtg ctgccgctgg
     3001 tgaagaagta tggcgcggct gtcgtggcga tctccaatga cgagacggga atctccgagg
     3061 atcccgacgt gcgcttcgcg gtggccacga agatcgtgca tcgcgccgcc gatcacggca
     3121 ttcccgcaag cgacatcgtg gtcgacccgc tcgtcatgcc aatcggagcg atcaatcagg
     3181 ccggtgtcca ggtcatgcat ctcgtgcggc gcctgcgcaa tgagctgaag gtgaatacca
     3241 cctgcggcgc ctcgaacgtg agcttcggcc tgcccgcgcg cgaaggcgtc agcgcggcct
     3301 tcctgaccat ggcgattggt gcgggcatga cctccgcgat catgaaccct ctgcacctgg
     3361 agatcgtcaa agcctgtatg gccgctgacg tcatgatggg tcacgaccct gactgcgctc
     3421 gatggatccg ccgcttccgc gaggccccgg tgatgcaggc cggtgggggc gaagcgaagc
     3481 cccaggaggg cgccgcccgc ggccggcgcg agggtggcca tcggcgccgt gtctgaatgc
     3541 ccaggcttcc agcgatctga ctgaaccttt ggaatgtgaa ccgcgcccca tgaccgattc
     3601 cgacgctctg gtggtcttca cgccgtccgg caagcggggg cggtttgcac tcggcacacc
     3661 actgctgcag gcggcgcgct ccctcggagt ggacgtggac tcggtctgtg gcggccgggc
     3721 gctgtgcggc cgatgtcagg ttctcgtcat ggaaggcgac tttcccaagc atggcgtcaa
     3781 ctcgcgctcc gagaaccttt cccccctgag tgcggcggag cgcagttaca gccggcggcg
     3841 acccctgccc gaggggcatc gcctctcctg tgcttcggtc gtccgcggcg atctggtgat
     3901 cgatgtgccg ccgagcagtc aggtgcaccg gcaagtggtg cgtaaggccg ccgacgcacg
     3961 cgtgatcact ctcgatccgc tcgtgcacct gcactacgtc gaagtgcagc aacctgacat
     4021 gcacgaccct tccggggatt tgcagcggct gatgaatgcc ctgcagacgg aatggaatct
     4081 cagtggcctc gagtgcgatc tcagggtgct ccagaacctg cagtccgcgc tacgcaaggg
     4141 cggatggcga gtcacggtcg cagtccatgg cggctctcag atcgtgggca tctggcccgg
     4201 tctgcacgag cggctcttcg gccttgcggt cgatgtgggc tcgaccacga tcgcagcgca
     4261 tctgtgcgat ctcgaatcgg gcgaagtcgt cgcctcgagc ggctcgatga atccgcagat
     4321 ccgcttcggc gaggatctga tgagccgggt gtcctactcg atgatgaacc cgggtggcgc
     4381 acagcagatg acccaggtgg ttcgcagcgc catcaatggg cttgcaggcg aggtggcccg
     4441 cgaggcgaac atctccatcc aggagatcct ggaggtgacc ttcgtcggca atcccatcat
     4501 gcatcacctg ctgctgggca tcgatcctgt ggagcttggg ggggcgccct ttgcgctcac
     4561 caccgatggc gccctcacga tgtgggccac cgagatcgac ctgcagctcc atccgaacgc
     4621 acgcatctac gtgctgccct gtatcgccgg tcatgtcggg gcggatgctg ccggcatgat
     4681 tctcgccgag cggcccgatc ttggagcgga attgaccctg ctcgtggatg tcggcaccaa
     4741 cgccgagatc gtgctgggca atcgcgaccg cctgctggcc tgctcgagcc caaccggccc
     4801 cgccttcgaa ggcgcgcaga tcagctgcgg ccaacgcgcc gctccgggag ccatcgagcg
     4861 agtgcgcatc gatccccaga gcctcga
//
LOCUS       DASRHN010000200         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1550340, whole genome shotgun sequence.
ACCESSION   DASRHN010000200 DASRHN010000000
VERSION     DASRHN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550340"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VFB37_07770"
     CDS             <1..1166
                     /locus_tag="VFB37_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22390.1"
                     /translation="LLEPGSESFKIYYEVSATTPGAKYFYNPIRKGSMASDESVRDAM
                     LGTPLQFEIVSGTDARKDPLMPDADPETSYIKVTLARAVPEHGQGRMVILKTYKDPKS
                     YHLDGTTLVFDRPLGVKRNKVVLPPGYEVVALSVPAQILTESDGRIAISFLHAGTGEA
                     PLIIRAVKDAQQGAAALPKAPTSRRSWEAPFAGETEVSRLSERAHQDRDIVYFLQQPQ
                     THAFRLYHDYTESRRGVNGYANVVRTGSVASNPSAYIVDTGEQLQAREMSGAQMVASR
                     INTGESVKAADHVVVIPFAAVEAGQTRRLRIEETYTAPASYRLEGDELVFDRSLGRPR
                     NAVVLPSGWYCTFSAAPATVTQLPDGRIRLDYWDDRPEAVDVLLKARRRFTGR"
     gene            1194..1595
                     /gene="crcB"
                     /locus_tag="VFB37_07775"
     CDS             1194..1595
                     /gene="crcB"
                     /locus_tag="VFB37_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016139391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZO22391.1"
                     /translation="MWKYILAISLGASAGALFRWWLAVQLNRYFPEIPPGTLAANLIG
                     GYIVGLAVALFASSSWSPEWRLLVITGFCGGLTTFSTFSAEIVALLQQGRSLWACAAA
                     LAHLVGSVVMTFAGIGTIGLLRLFAGASGER"
     gene            complement(1627..1998)
                     /locus_tag="VFB37_07780"
     CDS             complement(1627..1998)
                     /locus_tag="VFB37_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO22392.1"
                     /translation="MPGFDLVVVGAGMVGLATALAAAARRLRVLVIDREAQANECSWL
                     RAPELAAVLHSMTELRVESRGRAANCGVAFGRAWSDFHAQYRRSRRRDAERAHFARLT
                     ASAAGCHLSGGMTFVACTRSA"
     gene            complement(2024..>2704)
                     /locus_tag="VFB37_07785"
     CDS             complement(2024..>2704)
                     /locus_tag="VFB37_07785"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZO22393.1"
                     /translation="ATAEVWYRLLNLGFRIPAAGGTDAMANFASLRGPVGMNRVYAQV
                     PEGRLTQADFVDAIRHGHTFATNGPLLGFTLEGMQPGEELHLRGAPAHAAFVARLRSI
                     VPVDHLDLVCNGNVVRSLLRRPSTHADYQGEVPVGQPGWCVLRAWSEGAKYPVLDKYP
                     YATTSPVYVDIAGVTHRSPEDAAFFVAWIDRVTEATTAYPDWNNPGEKAHVLAQLSAA
                     KAFYLNMR"
BASE COUNT          510 a          905 c          838 g          451 t
ORIGIN      
        1 agctgctcga gcccggcagc gagagcttca agatctacta tgaggtctcg gcaaccaccc
       61 cgggcgcgaa gtacttctac aatcccattc gcaaggggag catggcaagt gacgagagcg
      121 tacgcgatgc gatgctcggc acgccccttc agttcgaaat cgtcagcggt acggacgccc
      181 gcaaggatcc tttgatgccg gatgccgatc ctgagacgag ctacatcaag gtcacgctcg
      241 ccagagcggt gccggagcac ggccagggca ggatggtgat cctcaagacg tacaaggatc
      301 cgaagagcta ccacctggac ggaacgacgc tcgtcttcga tcgccctctg ggcgtgaaac
      361 gcaacaaggt ggtcctgccg ccaggctatg aggttgtcgc actgagcgtg ccggcgcaga
      421 tcctcaccga gagcgatgga cggatcgcaa tcagctttct gcacgcaggc accggcgagg
      481 cgcccctcat cattcgcgcg gtaaaggacg cgcagcaggg tgcggcggct ctgccgaagg
      541 cacctaccag ccgccgcagc tgggaggctc ccttcgccgg ggagacggag gtctcgcgcc
      601 tgtccgaacg ggcgcatcag gaccgggaca tcgtctactt tctgcagcaa ccgcaaaccc
      661 atgccttccg gctctaccat gattacaccg agagccggcg tggcgtgaac gggtacgcga
      721 acgtcgtccg cacgggcagc gtggccagta acccctcggc ctacatcgtg gacaccggcg
      781 agcaattgca ggctcgggag atgagcggtg cgcagatggt tgcatcccgg atcaacactg
      841 gagagagcgt gaaggccgcc gaccacgtcg tcgtcattcc atttgccgct gtcgaggccg
      901 gccagacgcg gcgtctgcgg atcgaggaga cttacacggc cccagccagc tatcgcctgg
      961 aaggggatga gctcgtgttc gaccggagcc tcggaagacc ccgaaatgcc gtcgtgctgc
     1021 cctcgggctg gtactgcact ttcagcgcag cacccgcgac cgtgactcaa ctaccggacg
     1081 gccggattcg gctcgactac tgggacgatc gacccgaggc agtggatgtc ctcctcaagg
     1141 ccagacggcg gtttaccggg cgatagcccg gcaggcccca gcggagcgcc ggcatgtgga
     1201 aatacattct cgcgatcagt ctcggagcga gcgcgggagc ccttttccgc tggtggctcg
     1261 ccgtccagct caatcgctac ttccccgaga ttccccctgg cacgctcgct gccaatctca
     1321 tcggcggcta catcgtcgga ttggcggtgg cgctcttcgc ctcatcctcc tggtctcccg
     1381 aatggcggct gctcgtgatc accggctttt gtggcgggct caccactttc tcgaccttct
     1441 cggccgagat cgtggccctg ttgcagcagg gccgctcatt gtgggcctgt gcggccgccc
     1501 tggcacatct ggtgggctcg gtggtgatga cctttgccgg catcggcacg atcgggctgc
     1561 tgcggctttt tgcaggcgca tcaggagaac gatagcttca ctctcaacgg gtaagagctt
     1621 gggcggtcag gcgctgcgcg tacaggctac gaaagtcatc cccccggaca gatggcaacc
     1681 cgctgcgctc gcagtgagcc ttgcgaagtg tgcacgctcg gcatctctac gccgagaacg
     1741 gcggtactgc gcatgaaagt cactccatgc acggccgaaa gccacgccgc aattcgcggc
     1801 acggccgcgc gactcgaccc gaagctccgt catgctgtgg aggacggcgg caagctctgg
     1861 ggcccgcagc catgaacact cgttcgcctg ggcctcgcga tcgatcacca ggacacgcaa
     1921 ccggcgggcg gccgcggcaa gagccgtcgc aaggccgacc atgcccgcgc cgaccacgac
     1981 cagatcgaag cccggcacgt ttcgcccccc ccaccatggc acctcagcgc atgttcagat
     2041 agaacgcctt cgcagctgag agctgcgcca gcacgtgcgc cttctcgccc ggattgttcc
     2101 agtcggggta agcagtcgtc gcctcggtca cccgatcgat ccaggcgacg aagaaggcgg
     2161 catcctccgg tgaacgatgc gtgacccccg cgatgtccac ataaaccgga ctggtcgtag
     2221 cgtagggata cttatccaac accggatact tcgccccctc gctccaggcg cgcagcacgc
     2281 accaaccggg ctgcccgacc ggcacctcgc cctggtagtc cgcgtgagtg gaggggcggc
     2341 gcaggagcga ccgcaccacg ttgccgttgc aaaccagatc gagatgatcc acaggcacga
     2401 tggagcgcag ccgcgccacg aaggcggcat gtgccggcgc tccccgaaga tgcagctcct
     2461 cgcccggttg cataccctcc agagtaaacc ccaacaatgg accatttgtt gcaaacgtgt
     2521 gaccgtggcg gattgcgtcc acgaaatccg cctgagtcag ccgcccttcc ggcacttgtg
     2581 catacactcg gttcatccct acaggcccgc gcaatgaggc gaagttggcc atggcatccg
     2641 tgcctcccgc cgccggtatc cggaaaccca ggttgagcag gcgataccac acctcggcag
     2701 tggc
//
LOCUS       DASRHN010000201         6521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_68743, whole genome shotgun sequence.
ACCESSION   DASRHN010000201 DASRHN010000000
VERSION     DASRHN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68743"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..279)
                     /locus_tag="VFB37_07790"
     CDS             complement(130..279)
                     /locus_tag="VFB37_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016715543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HZO22394.1"
                     /translation="MSLRTLLIIVVILLLIGALPTWPYSAGWGYYPSGGLGLVLLIII
                     VLALL"
     gene            459..1958
                     /locus_tag="VFB37_07795"
     CDS             459..1958
                     /locus_tag="VFB37_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO22395.1"
                     /translation="MNGPAWRSLFARRPVELLQQQLEADAQRPLLRRVIGPIDLMLMG
                     VGVIVGAGIFVVTGTAAALYAGPAITLSFLLAGLACALSAMCYAELAAMIPAAGSAYT
                     YAYTTLGEVVAWVIGWNLVLEYVFAASYVAVGWSGYLGSFLQHWGLSLPPSLVRAPLD
                     SVDGRIVWTAGLNLPAIAIVLCMAGVALCGIALSRTVNVVIVSLKLAALSLVIVLGAT
                     HIDPGLWHPFVPPAEGSDGFAGGHFGWGGVLRGAGVIFVAYLGFDAIATTAQETRNPQ
                     RTLPIAILGALGLATLLYIVVSLVLTGLVPYRTLNVPDPLSTALRSTGPGLAWLVTVI
                     DAAAVIGLASVILVVMLAQARILFAMSRDGLLPPLLGRIHARTRIPHWSTSFSAVAIA
                     ILAGCFPIGILVQLVSIGTLGIFIVVCAGVIILRRTRPELPRRFRSPWVPVLPILGIL
                     VCGYILIGLPSRVWHLYGVWVAAGLLIYAFYGYRGARASRPGTAHDLVV"
     gene            1942..2898
                     /locus_tag="VFB37_07800"
     CDS             1942..2898
                     /locus_tag="VFB37_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZO22396.1"
                     /translation="MISLSDVEAAAARIHGHVRRTPVLRVGAARLDEALVGWSLPQEL
                     YLKLELLQVTGSFKPRGAFNRALLEPREALRGLVTSSGGNHGIAMAHVGRELGVPVAV
                     PLFATVSPEKVARMRALGAEAEIVGPTFQVSNARAREIAGERNYLYIHPFAEGPGIAG
                     AGTIGLEILQDVPEVDVVVIAVGGGGLLGGVSLAIKERRPQVRILGVEPTGADALTRS
                     LEAGQLVTLDRINTKAATLAPPASEQINLELARRYVDEMVHVTDEEMQQTAQWLWQQF
                     AIPTELAGAASTAALLTGRARVKPGSRVVSIVCGAGAAGWTA"
     gene            2886..3200
                     /locus_tag="VFB37_07805"
     CDS             2886..3200
                     /locus_tag="VFB37_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22397.1"
                     /translation="MDRLEGQRGAQAMGRGLVGTVTSLIATGLLLAPTARGEEPWFTR
                     FEQGRAQTNAACHEKKYEACAQGLRTLLPLVDGRPDIQCVLAAAQAQLGEQQASAQSL
                     RI"
     gene            3539..4015
                     /locus_tag="VFB37_07810"
     CDS             3539..4015
                     /locus_tag="VFB37_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZO22398.1"
                     /translation="MSSPSFADNGKLAMKNAGDDKSNPNCVGQNVSPALKWTHPPAGT
                     RSYALILFDPEGRGGLGVVHWVAYGIPASVTGFAEGEVSKPSPKYIGGKSTKNVPNYS
                     GPCTPPGDWHHYTFTLIATDLDPKELQPGMTREELFTALNGHTKGAAGLIGRFRHP"
     gene            4052..5011
                     /locus_tag="VFB37_07815"
     CDS             4052..5011
                     /locus_tag="VFB37_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZO22399.1"
                     /translation="MLSSSFRPSLEEFSRAAAAIEHDAIQTPLLPFRATSATDIRLKA
                     ENLQPLGSFKIRAGANALAMLPEAEAAAGVATASAGNFAQGLALAAARRRIKLTVHAP
                     SSAPAVKLAAIRQLGASIETHPYNEWWQIMSTRHTGRDDGRFIHPVCETGVLLGNGTI
                     ALELVKQWACIDTVFVPVGGGGLLCGIALAFRALGARPRIIACEVETAAPLAQSRQAG
                     RPVDIQRQPSFVDGIGGGGVLPEMWPLLQDLVDAVVVVSLDEIRQAMRVLALENHLVC
                     EGAGAASVAAALSGRGGDGNCVAIVSGGNIDLSVFTDIVNMPV"
     gene            5304..>6521
                     /locus_tag="VFB37_07820"
     CDS             5304..>6521
                     /locus_tag="VFB37_07820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO22400.1"
                     /translation="MESAAARTLTQTPRIYWLTEAYFPPQLGGLELMVARLSEGLAAR
                     GLEVHVVTRLPDPQYPKEQYIAGVHVRRIPPAGRLKGIGWRALPRVIEYLARLAIMLV
                     RDARHYDVVIVSGLKVIPLIAVPLCHLLGKRCIVRVESTYELLEPVSAMSAEAMKGSA
                     ARMVQRFSEWIQRVMLFNADRVIATSNELQELVLEAGVQPEKISMIFNAVDLRRFHPV
                     SRSEKERLRQELHLPRKATIVLFAARLSRAKGISLLVEAWPQLAAQHSDLCLVIVGAG
                     HGSFDDCEAELVQFLQLTGLAGTRVLMQGPSERVDQYLQAADLYILPSEYEGFGVGVI
                     EALATEIPVLATPVGVVPQLIQDGDNGFVFPPRDEPALIEAVETALAERHRWPEIVKR
                     ARKGVSSMDVGAVV"
BASE COUNT         1058 a         2112 c         2129 g         1222 t
ORIGIN      
        1 tttcgagcag aagacccact ggcgcaaacc gccgccagat ttcgcgtcac tgcatggcgc
       61 gccagcgagt accccctgat cgactgcacg caaagcgcgc ctaagccgcg cgcctgggca
      121 gccgcccggt cataacaacg cgagtacgat gatgatcagc aacaccagtc ccaaaccgcc
      181 gctcgggtaa tagccccagc cggcgctgta tggccaggtg ggaagcgccc cgatcagcag
      241 taggatcacg acgatgatca gcagggttcg taaggacatg ggttacccgc cctgaaggtc
      301 cctggcatca actcgccgcc gagggggaag ttccagccct tccgggcgcg gcaagtgcaa
      361 cgtggcgccc gtggctggct gcttgcagtg ggacagggcc gagcccagcg gctaccatac
      421 gcccgcaaca attcggcaag gtcctggagc gctactcgat gaatggcccc gcctggcgct
      481 cgctctttgc ccgtcggccc gtggagctgc tgcagcaaca gctggaagcc gacgcgcagc
      541 ggcccctcct tcgccgggtc atcggaccca ttgacctgat gctgatgggc gtcggggtga
      601 tcgtcggggc cggtatcttc gtcgtcacgg gaacagcggc ggcgctctac gccggtcctg
      661 cgatcacgct gtctttcctg ctcgcaggtc tcgcgtgcgc cctgtctgcg atgtgctacg
      721 cggagctggc tgccatgatc ccggccgccg ggagcgccta tacctacgcg tacacgactc
      781 tcggggaagt cgttgcctgg gtgatcgggt ggaacctggt tctggaatat gtgtttgccg
      841 cttcctatgt cgcggtgggt tggtccggct atctcgggtc ttttctgcag cactgggggc
      901 tgagcctgcc gccctccctg gttcgggcgc cgctggacag tgttgacgga cggatcgtct
      961 ggacagccgg actgaacctt ccggcgatcg caatcgtgct gtgcatggcg ggagtcgccc
     1021 tgtgcggcat cgcgctttcg cgaacggtga acgtcgtcat cgtgagtctg aagctcgccg
     1081 ctctatcgct ggtgatcgta ctgggtgcca cccacatcga tccgggcctg tggcatccct
     1141 tcgttccgcc cgccgaaggc agcgatggat tcgcgggcgg ccactttggc tggggcggcg
     1201 tgctgcgcgg cgccggggtg atcttcgtgg cttatctggg attcgatgcc attgccacga
     1261 cggcccagga aacccgcaat ccacagcgca cgttgccgat tgccattctg ggcgcgctcg
     1321 gcctggccac cctgctctat atagtggtgt ccctggtcct gaccggtctc gtgccgtacc
     1381 ggacgctcaa cgtgccggat cccttatcca ccgccttgcg ctcgacgggt ccggggctgg
     1441 cctggctggt caccgtgatc gatgcggcgg ccgtcatcgg actggcctcg gtgattctgg
     1501 tcgtgatgct cgcacaggcg cgcatcctgt tcgcgatgag tcgcgacggg ctcttaccgc
     1561 ctctgctcgg ccggattcat gcgcggacgc gcatccctca ctggagcacc agcttttcgg
     1621 ccgtcgcgat tgccatcctg gcaggctgct ttccgatcgg cattctcgtg cagctcgttt
     1681 cgatcgggac gctcgggatc ttcatcgtag tgtgcgcagg tgtcatcatc ctgcgccgca
     1741 cgcggcctga gctgccccgg cgcttccgtt cgccctgggt gccggtactg ccgatactcg
     1801 gaatcctggt ctgcgggtac attctgatcg ggctgccctc ccgcgtctgg cacctgtacg
     1861 gcgtctgggt cgcggcaggg ctgctgattt atgcgttcta cggttatcgc ggtgcgcgtg
     1921 cctcgcgccc agggacagct catgatctcg ttgtctgatg tagaagcggc ggcagcacgc
     1981 attcacgggc acgttcggcg cacgccggtg ctgcgcgtcg gcgcggcgcg actcgatgag
     2041 gctctcgtcg gctggagtct tccgcaagag ctctatctga agctggagct tctgcaggtc
     2101 accggctctt tcaagccacg cggcgcattc aatcgagcac tgctcgagcc gcgtgaggcg
     2161 ctgcgcggac tcgtgacctc atctggcggc aatcacggga ttgccatggc gcatgtcgga
     2221 cgcgagctgg gcgttccggt tgccgttccg ctctttgcca cggtcagccc ggaaaaggtg
     2281 gcccggatgc gggcgctggg cgccgaggcg gaaatcgtcg gcccgacttt ccaggtttcc
     2341 aacgcccggg cacgagaaat cgcgggcgag cgcaactacc tctatatcca tcccttcgcc
     2401 gaaggcccgg gtatcgccgg tgcggggacc atcggcctgg aaatcctcca ggatgttccg
     2461 gaggtggatg tggtggtcat cgcagtgggc ggcggcggcc tgctcggggg tgtttcactc
     2521 gcgatcaagg aacgacgacc gcaggtccgg atcctcggcg tcgagccaac cggcgcagac
     2581 gcactgaccc gcagcctcga ggccgggcag ctggttacac tcgatcgcat caataccaag
     2641 gcggccacgc tcgctccgcc ggcatcggag cagatcaatc tcgagctggc gcggcgctac
     2701 gtggatgaga tggtccatgt cacggacgag gagatgcaac agactgccca gtggctgtgg
     2761 cagcagttcg ccatacccac ggagctggca ggcgcggcct ccacggccgc cttgttgacg
     2821 gggcgggcgc gcgtgaaacc cggaagccgc gtcgtcagca tcgtttgcgg agccggagcg
     2881 gcgggatgga ccgcctagag gggcagcgcg gtgcccaggc gatgggccgc gggctcgtgg
     2941 gcacggtgac cagtctgatc gcgactggcc tgctgctcgc cccgacagct cgaggcgagg
     3001 agccgtggtt cactcgcttc gagcaaggtc gtgcgcagac caatgcggcc tgccacgaga
     3061 agaaatacga ggcctgtgcc cagggcctgc gtactttgct accgctggtg gatggacgac
     3121 ctgatatcca gtgcgtgctc gcggcggccc aagcccagct cggcgagcag caagcctctg
     3181 cacaaagctt gcgcatctga tcgcttatga gccggtggcc tcgtcatttt tcgtaagcag
     3241 cgtgcgcgaa cgcaagatcg tgcgcatcgc gcgcgatggt acggtctccg atttcatcac
     3301 cgccgcgcag gtgcccgcgt ggggcctgtt tgtgctcgca agcctttagg cctgaggcga
     3361 tcacgccttc cgaggcaagc tgaggaggcc tcgccgcggt atgctgccgg accagcgcgc
     3421 agattttgcc aatcgaccaa tggagaacaa cgtgaaagca ctactgggat ctctgctcgg
     3481 ggtattactt gcattgccca cattcgcagc gggagaggcg tcaagcggtg cattcgcctt
     3541 gagctcgccg agcttcgcgg acaacggcaa gctggcgatg aagaacgccg gtgacgacaa
     3601 gtcaaacccg aactgcgtcg gacagaacgt ctcaccggca ctcaagtgga cccacccgcc
     3661 agcgggaaca cgcagctatg cgctgatcct gttcgacccc gagggccgag ggggcctggg
     3721 cgtcgtgcat tgggtcgcct acggcattcc ggcatccgtg accggattcg ccgagggcga
     3781 agtcagcaag ccctcgccga aatacatcgg cggcaagagc acgaagaacg ttcccaatta
     3841 ctctggaccc tgcactccgc ccggcgactg gcatcactac accttcacgc tgatcgccac
     3901 cgacctcgat cccaaggagc tgcaacccgg aatgacgcgc gaggaattat tcaccgcgct
     3961 gaatgggcat accaaggggg ccgctggact gatcggccgc tttcgtcatc cctgatacga
     4021 gcctgccccg cctgagacgg ccgcattcaa catgctctcc agcagtttcc gcccctccct
     4081 cgaggagttc tcgagggccg ctgcagccat cgagcacgat gccatccaga ccccgctgct
     4141 gcctttccgg gcgacatccg ccaccgacat ccgtctgaag gccgagaatc tgcagcctct
     4201 cgggtcattc aagatccggg ctggcgccaa tgcgctggcc atgctgcccg aggcggaggc
     4261 ggctgcgggg gtcgccacgg cgagcgcggg aaacttcgcg cagggactgg cgctggcggc
     4321 ggctcgccgc cgcatcaagc tgaccgttca cgcaccgagc agcgctccgg cggtgaagct
     4381 cgccgcgatc cgtcaactgg gcgcgagcat cgagacccat ccctacaatg agtggtggca
     4441 gatcatgtcc acgcgccaca ccgggcgcga tgacggccgg ttcatccacc cggtgtgcga
     4501 gacgggtgtg ctgttgggaa acggaacgat cgcgctcgag cttgtaaagc agtgggcctg
     4561 catcgatacg gtattcgtgc ccgtcggtgg cggcgggttg ttatgtggga ttgcgctcgc
     4621 gttccgggct cttggtgcgc gcccacgcat catcgcctgc gaagtcgaga ctgcggcacc
     4681 ccttgcgcaa tcgcgacagg ccggccggcc cgtcgatatt cagcgacaac cttccttcgt
     4741 cgatggcatc ggcggcggcg gtgtgctgcc ggagatgtgg cccttgctgc aggacctggt
     4801 ggacgccgtt gtcgtcgtca gtctcgatga gattcgccag gcaatgcgcg tactggcgct
     4861 cgagaatcac ctcgtgtgcg aaggggccgg cgctgcatca gtcgcggccg ctctcagcgg
     4921 ccgcgggggt gatggcaatt gcgtcgccat cgtgtcaggc ggcaacatcg acctgtcggt
     4981 cttcaccgac atcgtcaaca tgccagtttg atccgccctg cgccgctccc tgtgcgcgcg
     5041 cccaagtagc tccctgcctc gccgatcaga cgcgctgccg ctgcagcctc ttgctcggcc
     5101 accgcctgcg gtccacctga ggctcgagcg cggtgctcca acacgggcac aagacctgct
     5161 tcggaagcga ggcggccgcc tccaacgttc agcccacggt tgaatcgttc gggctgcgtc
     5221 gcagcttgcg caggccgtcg cagctcaccg aggcgcacgg tttgaatcga taaacagagt
     5281 tgaattgata cccgaggagc gtgatggaat cagccgcagc gcgtaccctg acacagaccc
     5341 cgcgaatcta ttggctgacc gaggcgtatt ttccgcccca gctcgggggc ctcgagctga
     5401 tggtcgcgcg cctttcggag ggtctcgccg cgcgtggact cgaggtccat gtcgtgacgc
     5461 gactgcctga cccgcaatac ccgaaggaac agtacatcgc cggcgtgcat gtgcggcgca
     5521 ttccgcccgc ggggcggctc aaaggaatcg gatggcgcgc cctgccgcgg gtcatcgagt
     5581 acctcgcgcg cctggcgatc atgctggtgc gcgatgcgcg acactatgac gtcgtcatcg
     5641 tctctggttt gaaagtcatc ccgctcatag ccgtgcccct ctgtcacctc ctcggcaagc
     5701 gatgcatcgt gcgcgtcgag tccacgtacg agcttctcga gccggtctcg gcgatgagcg
     5761 ccgaggccat gaagggctcg gcggcgagga tggttcagcg cttctcggag tggatccagc
     5821 gtgtgatgct cttcaatgcc gatcgggtga tcgccacctc gaacgagctg caggagctgg
     5881 tccttgaggc cggcgtgcaa ccggagaaga tcagcatgat cttcaatgcc gtggacctgc
     5941 gccgcttcca tccggtctca cgcagcgaaa aggagcgcct gcgccaggag cttcatctgc
     6001 cacgcaaggc aacgattgtt ctttttgccg cccggctctc gcgcgccaag ggaatcagtc
     6061 tgctcgttga ggcctggccg cagcttgccg ctcagcattc ggacttgtgc ttggtgattg
     6121 tcggcgccgg tcatgggtca ttcgatgact gcgaagccga gctggtgcag tttctgcaac
     6181 tcaccgggct cgcgggcacc cgtgtgctga tgcaaggccc gagcgagcgg gtcgatcaat
     6241 acctgcaggc cgcggatctg tacatcctgc cgagcgagta cgagggattc ggcgtcggcg
     6301 tcatcgaggc gctcgcgacc gagatcccgg tgctggcgac gcccgtcgga gtcgtgccgc
     6361 agctgattca ggacggcgac aatggtttcg tcttcccccc tcgcgatgag cccgcgttga
     6421 tcgaagccgt cgagacggcg ctcgccgagc gacatcgatg gcccgagatc gtcaaacgcg
     6481 cccgcaaggg cgtctcttcc atggatgttg gagcagtcgt g
//
LOCUS       DASRHN010000202         6537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1055690, whole genome shotgun sequence.
ACCESSION   DASRHN010000202 DASRHN010000000
VERSION     DASRHN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055690"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFB37_07825"
     CDS             <1..943
                     /locus_tag="VFB37_07825"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HZO22401.1"
                     /translation="NKLKDLPATDRFHFVEGDITINKEWVEYHVKKCDAVVPLVAIAN
                     PAQYVTHPLRVFELDFEANLAVVRYCVKYGKRLIFPSTSEIYGMSEDSVLDEETSPLV
                     YGPVNKQRWIYAASKQLLDRVIYAYGVRDDLDYTLFRPFNFIGPNLDNIEEPKEGSSR
                     VFTQFLSNVLYKRPIKLVDGGRQKRSFTFIDDAVEALLTILENQDGRATRRIFNIGNP
                     DNCVSIRDLAERTIRIAQEFPVLRANARETTIVDVSAGEYYGKYYQDIQVRVPAIEAA
                     KRDLGWKPTTDLDTAIRRTIEYYVNQEGFGTRSIANI"
     gene            972..2615
                     /locus_tag="VFB37_07830"
     CDS             972..2615
                     /locus_tag="VFB37_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZO22402.1"
                     /translation="MSTQSGQELRTLRGSGWALWILLAVVWFATMPWRPLLDPDEGRY
                     AEIPREMLSSGDWITPRLDGLKYFEKPPLQYWATAATYSVFGLSEWTARLWTVGLGFL
                     CLPMVLAFASRLRGRAAGLCALTALAVSPYFEIVGHLNLLDAGFTFWLTATAFAFVLA
                     QCEPPRSAAQRRWMLLAWGAAALAVLTKGIVVGVLTGASLALYSLVERDYRPWRRLHL
                     VRGGALFLLIALPWFIAVSVRNPSFLKFFFVHEHIARFLTTVHQRVEPWWYFAVLLLI
                     GTLPWLTRLPGSVAAGWRESEQNDRFKPLKFLLIFCVVTLLFFSASGSKLAPYILPIM
                     PPLAVIAAAGLPNPASFIRRTALFTGVLLLLIAVGLVIYSLRRNGYFPHEALPWLLAG
                     SAAALCSVLIQWFPRTAATMTLALATAGLAVLGCQCLLGAYTQVPPQRSAYRLARAVR
                     PLIHRQTELYSLGQYRETLSPYLQRTLQLVQFQGELAFGLSEEPAKDLTGDAFLTHWN
                     QATDAIAFLDPSMLSWWRQHGLKGRVIAGDQDTVAVSRL"
     gene            2612..2986
                     /locus_tag="VFB37_07835"
     CDS             2612..2986
                     /locus_tag="VFB37_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZO22403.1"
                     /translation="MRLSDFAVLSTGVLLNALAQLGLKAATRVTGPLLVADGGGTWSR
                     AWQLVGVPALWFALAAYGLSVIVWLIGLSRVPVSQAYPLLSLGYVINIGLAWWLLGEI
                     PNAQRVVGIGVIVAGVVLVARS"
     gene            3006..4121
                     /locus_tag="VFB37_07840"
     CDS             3006..4121
                     /locus_tag="VFB37_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HZO22404.1"
                     /translation="MLPFTRPTIDEETIQGVAEVLRSGWLASGPKVAEFESALSRYLG
                     GRPVRTQTSATASMEMALLACGIGKGDEVITPAMSFVATANVIVRVGAHPVFVDVGLD
                     SRNIDFEQVEAAITPRTRAILPVHFAGLPCDMDRLYALARRHHLRVIEDAAHAIGASW
                     KGQRVGSFGDLVCFSFHPNKNITTIEGGAVSGGSPEEVKSLELHRWHGQSKSAAGTDT
                     LFAGGKANLSDVAARVGLGQLARLEEFNAKRRERVARYFDLWRADPPVRLPERGDAGH
                     GWHLFAPLLPLDRIRLSRAQFIEAMSARGIGVGVHYPAIHLFTAYRALGYREGQFPNA
                     ERIGRETITLPLFPAMELSDVERVVRAVTEVIEGARR"
     gene            4171..5175
                     /locus_tag="VFB37_07845"
     CDS             4171..5175
                     /locus_tag="VFB37_07845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO22405.1"
                     /translation="MNATLSVVIPVYNEEGGLQMLFDRLYPVLDGLARQYEVIFVDDG
                     SADRSVAVLREQFGRRPDSTRVMVLARNVGQHMAILAGFAQARGDYIITLDADLQNPP
                     EEIARIVAAMDAGADYVGTVRRRRHDHLWRKAASRLMNRIRERTTQIRISDQGCMLRG
                     YDREIVRAINSCVEVSTFVPALGFTFARHPVEIEVAHAERQVGQSKYSLYHLIRLNFD
                     LMTGFSVMPLQFFSMAGIAIAVLSLIGVVLLAIRRLVVGPEAEGVFTLFGIAFFLIGV
                     TLMGVGVVGEYVGRIYEQVRQRPRYTVAALLEQSEAVTPGAGASEARLLTDDRATGSR
                     "
     gene            5176..6105
                     /locus_tag="VFB37_07850"
     CDS             5176..6105
                     /locus_tag="VFB37_07850"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HZO22406.1"
                     /translation="MAAARAVVFAYHDVGVRCLLALLSADIEIPLVVTVADDPHETRW
                     FGSVADTAAEHDLRVVRPADPNTGELAQTLSELQPDFIFSFYYRALLGGALLRTAARG
                     ALNMHGSLLPKYRGRAPVNWAILRGEKETGATLHYMVERADAGDIVDQLAVPILHEDN
                     AQEVFRKVTVAAETVLARSLPALLAGTAPRRSQPPAPGEYFGRRRPEDGQIDWSRSAQ
                     EIHNLVRAVAPPFPAAFTTIRSQRWWLHATRVEPAMRQPAAAAQLTGEQGRCLIICRD
                     GSVLRLLAAVTAAGPVDLAAVAQAAQKSPVTLQ"
     gene            6161..>6537
                     /locus_tag="VFB37_07855"
     CDS             6161..>6537
                     /locus_tag="VFB37_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZO22407.1"
                     /translation="MRRIALKIDVDTDRGTLEGVPRLATLLERLDARATFLFSLGPDQ
                     TGRALRRVFRRGFLGKVRRTSVVQHYGVKTLLYGTLLPGPHIGRRRGALMREIAKSGF
                     EVGVHTYNHSKWQDGVATAGESWT"
BASE COUNT         1055 a         2064 c         2136 g         1282 t
ORIGIN      
        1 caacaagctc aaggatctgc ccgccaccga tcgattccac ttcgtcgaag gcgacatcac
       61 catcaacaag gaatgggtgg agtaccacgt caagaagtgc gatgccgtcg tgcccctggt
      121 cgccatcgcc aatcccgcgc aatacgtcac gcacccgctg cgtgtcttcg agctcgactt
      181 cgaggccaac ctcgccgtgg tgcgttactg cgtcaagtac ggcaagcgcc tgatcttccc
      241 gtccacttcg gagatctatg gcatgtcgga agacagcgtg ctggatgagg aaaccagccc
      301 gctggtctat ggtcccgtca acaagcagcg ctggatctac gcggcctcca agcagctcct
      361 cgaccgggtg atctacgcct acggtgtacg cgacgacctc gattacacgc tcttcaggcc
      421 tttcaatttc atcgggccca acctggacaa catcgaagag ccgaaggaag gcagctcgcg
      481 cgtgttcacc cagtttcttt ccaacgtgct gtacaagcgg cccatcaagc tcgtcgatgg
      541 cggccgccag aagcgctcct tcaccttcat cgacgatgcg gtggaggcgc tgctcaccat
      601 tctcgagaat caggacgggc gggcgacccg gcggatattc aacatcggca atcccgacaa
      661 ctgcgtctcg atacgcgatc tggccgagcg gactatccgg atcgcgcagg agttccccgt
      721 gctgcgggcc aacgcgcgtg agaccaccat cgtggatgtt tcggcgggcg agtattacgg
      781 caagtactat caggatatcc aggtgagggt gccggccatc gaggcggcca agcgcgacct
      841 cggctggaag ccgaccaccg acctcgacac cgccattcgt cgtacgatcg agtactacgt
      901 gaaccaggaa ggtttcggta cccgctcgat cgccaacatc tgaggcggca ttcgcagcgg
      961 ccgggtgttg gatgagcacg cagtccggtc aggagctgcg caccttgcgc ggctccgggt
     1021 gggcgctatg gatcctgctg gcggttgtgt ggttcgccac gatgccctgg cggccgctgc
     1081 tcgatccgga tgaaggacgc tacgccgaga tcccgcgcga gatgctgtca agcggcgact
     1141 ggatcacgcc gcgtctggat ggcctgaagt acttcgagaa gccgccgcta cagtactggg
     1201 ccacggcggc aacctactcc gtgttcggcc tgagtgaatg gactgcgcgc ctgtggacag
     1261 tggggctcgg ttttttgtgc ctgccgatgg tgcttgcgtt cgcgagccgg ctgcgcgggc
     1321 gcgcggcggg gctgtgtgcc ctgaccgcgc tcgcggtcag cccgtacttc gagatcgtgg
     1381 gacacctgaa cctgctggac gccgggttca ccttctggct cacggcgact gcgttcgcct
     1441 tcgtgctcgc gcagtgtgag ccgccccgat ccgcagcgca gcgccgctgg atgctgctcg
     1501 cctggggggc cgcggccctc gccgttctga ccaaggggat cgtggttggg gtgttgacgg
     1561 gggccagcct ggcgctctac agccttgtgg aacgcgatta ccgcccctgg cgacgcctgc
     1621 acctcgttcg tgggggcgcc ttgtttctgc tgatcgctct gccctggttc atcgccgtca
     1681 gtgttcgtaa tccttcgttc ctgaagttct tcttcgttca cgaacacatt gcacggttcc
     1741 tgacgacagt gcaccagcgt gtggaaccct ggtggtattt cgctgtgctg ctgctcatcg
     1801 gcacgctgcc ctggctcacc cgcctgcccg gcagtgtggc ggccggctgg cgagaatccg
     1861 agcaaaatga tcgtttcaag ccactgaaat tcctgttgat attttgcgtc gtgaccctgc
     1921 tatttttctc cgcctcgggc tccaagctgg caccttacat cctgcctatc atgcctccgc
     1981 tagcggtgat cgccgctgcg gggctgccga atcccgcctc gttcatcagg cgcacggcgc
     2041 tcttcactgg ggtgttgctg ctgctcatcg ctgtgggact ggtcatctac tctctccgcc
     2101 gtaatgggta tttcccccac gaggctctcc cgtggttact ggccggcagc gcagcggccc
     2161 tctgctcggt gctgattcag tggtttccac gcacagcggc caccatgacg ctcgcattgg
     2221 ccacggctgg cctcgccgtc ctcggctgtc agtgcctgct gggcgcctac acgcaggtcc
     2281 cgccacaacg ctcggcctat cgcctggcac gagccgtgcg gccgctcatc cacaggcaga
     2341 ccgagctgta cagcctggga caatatcgcg agaccctctc gccctacctg cagcgaacct
     2401 tgcagcttgt acaattccag ggcgagctcg ctttcgggct gagcgaggag cctgcgaagg
     2461 acttgacggg ggatgcgttt ctgactcact ggaaccaagc gaccgatgcc atcgccttcc
     2521 tggacccctc catgctgagc tggtggcgcc agcacggttt gaagggccgg gtgattgccg
     2581 gtgatcagga cacagtggcg gtcagcagac tatgaggctc agcgacttcg ctgtgctctc
     2641 gaccggtgtc ctgttgaatg cgctcgcgca gctcgggctc aaggctgcta cgcgcgtcac
     2701 cggaccgctg ctcgttgcgg atgggggcgg cacctggagt cgggcctggc agctggtggg
     2761 tgtgccggcg ctgtggtttg ccctggctgc ttatggtctg agtgtgatcg tatggctgat
     2821 tggcttgtcg cgggtcccgg tgagtcaggc ctatccgctg ctgtcgctcg gctacgtgat
     2881 caacatcggg ctcgcgtggt ggctgctcgg agagattccg aacgcccagc gggttgtggg
     2941 catcggcgtc atcgtggccg gcgtggtgct ggtcgcgcgc tcgtaagagg accatcgtga
     3001 ccaaaatgtt gcccttcacc cggccgacga tcgatgagga gaccattcag ggcgtggccg
     3061 aagttttgcg ctcgggctgg ctggcaagtg gtcccaaggt cgctgaattc gagtcggcac
     3121 tgtcgcgcta cctgggcggg cggccagtgc gaacccagac ctctgcgact gccagcatgg
     3181 agatggctct tctggcctgc ggcatcggca agggcgatga agtcatcacc cccgccatga
     3241 gcttcgtggc cactgccaat gtcatcgtcc gggtcggcgc gcatccggtg ttcgtggacg
     3301 tcggactcga ctcgcgcaac atcgatttcg agcaggttga agcggcgatc acgccccgca
     3361 cgcgcgccat cctgccggtg catttcgccg ggctgccgtg cgacatggac aggctgtacg
     3421 cgctggcgcg ccgtcatcat ttacgggtga tcgaggatgc ggcgcacgcc atcggcgcct
     3481 cgtggaaggg acagcgggtc ggcagctttg gcgatctggt gtgcttcagc tttcatccca
     3541 acaagaacat caccaccatc gagggcgggg ccgtcagcgg cggatcgccc gaagaggtca
     3601 aaagtctcga gctgcatcgc tggcacggtc agagcaagag tgccgccggt accgataccc
     3661 tgttcgccgg cggcaaggcg aacctgtccg atgtcgccgc ccgcgtcggg ctcggacagc
     3721 tggcgcggct cgaggaattc aacgcgaagc ggcgcgagcg ggtcgcccgc tacttcgatc
     3781 tgtggcgcgc ggatcccccg gtccgactgc cggagcgggg cgatgccgga catgggtggc
     3841 atctgtttgc tccgctgctg ccgctggatc gcatccgcct cagccgcgcg cagttcatcg
     3901 aggccatgag cgcacgcggc atcggtgtcg gggtgcatta tccggccatc catctgttca
     3961 cggcctatcg tgcgctcggc taccgcgagg gccagtttcc caacgcagag cggatcggcc
     4021 gggaaaccat cacgctgccg ctctttccgg ccatggagct gagcgatgtc gagcgtgtcg
     4081 tccgcgctgt gaccgaagtc atcgagggag cgcgccggtg agccggaacg cgccgctcag
     4141 ccggatggtc atcactccgg gagcctcgcg atgaacgcga ccctttccgt cgtcattccc
     4201 gtctacaacg aggagggcgg cctgcagatg ctcttcgatc gtctgtaccc ggtccttgac
     4261 ggattggcac gccagtatga agtgatcttc gtcgatgacg gcagcgcgga ccgctccgtg
     4321 gccgtactgc gtgagcagtt cggacggcgc cccgactcaa ctcgggtcat ggtgctggcg
     4381 cgcaatgtgg gccaacacat ggcgatcctg gccggattcg cgcaggcgcg cggcgattac
     4441 atcatcaccc tggacgctga tctgcagaac ccgccggagg agattgcccg gatcgtggcg
     4501 gccatggatg cgggcgcgga ctatgtcggc acggtacgcc gccggcggca cgatcacctg
     4561 tggcgcaagg cagcctcccg gctcatgaat cgcatccgcg agcgaaccac tcagatccgc
     4621 atcagcgatc agggttgcat gttacgcggc tacgatcggg agatcgtgcg ggccatcaac
     4681 agctgcgtcg aggtaagtac cttcgtgccg gcgctgggct tcacctttgc ccgccatcca
     4741 gtggagatcg aggttgctca tgccgaacgg caggtcgggc aatcgaagta ttccctgtat
     4801 cacctgatcc ggctcaactt cgacctgatg accggctttt cggtcatgcc cctgcagttc
     4861 ttctcgatgg ccggcatcgc gatcgccgtg ctctcgctga tcggcgtggt gctgctggcc
     4921 attcgccgcc tcgtcgttgg accggaggca gaaggtgtct tcaccctgtt cggcatcgcc
     4981 ttctttctca tcggtgtcac gctgatgggc gtgggggtcg tgggcgagta tgtcggtcga
     5041 atctacgaac aagtgcggca gcgcccgcgt tatacggtgg cggctctgct cgagcagtcg
     5101 gaggccgtca cacccggggc aggcgcaagt gaggctcgcc tgctgacgga tgaccgggcg
     5161 accgggagcc gctgagtggc tgctgcccga gcagtcgttt tcgcctatca cgatgttggg
     5221 gtgcgatgtc tgctcgcgct gctctccgcc gacatcgaaa ttccgctcgt cgtcaccgta
     5281 gcggacgatc cacacgagac ccggtggttc ggcagcgtcg ccgataccgc ggccgagcac
     5341 gatctgagag tcgtgcggcc ggccgatccc aacaccggcg agctggcgca aacgctctcc
     5401 gagctgcagc cggattttat cttctctttc tactaccgcg ctctgctcgg cggtgcgctg
     5461 ctgcggacgg ccgctcgggg cgccctgaac atgcacggat cgctgttacc caagtatcgg
     5521 gggcgcgcac ctgtcaactg ggcgattttg cgtggggaaa aggaaacagg cgcgacgctt
     5581 cactacatgg tggagcgtgc cgatgccggc gatatcgtcg atcagttggc ggtgccgatc
     5641 cttcacgagg acaacgcaca ggaagtcttt cgcaaggtca ccgtagcagc cgagacggtg
     5701 ttggcgcgct cacttcctgc actgctggct ggaactgccc cgcggcggtc ccaacctcca
     5761 gcgcctgggg agtactttgg tcggcgccgt ccagaggatg ggcaaatcga ctggagtcgt
     5821 tcggcccagg agattcacaa cctggtgcgc gctgtcgcac cgccgtttcc cgcggcattc
     5881 acgaccattc gatcgcagcg ctggtggctg cacgctacgc gagtcgagcc cgcgatgcgc
     5941 cagcccgctg cagctgcgca actgaccggt gagcagggtc gctgtctgat catctgtcgc
     6001 gacggcagcg tgctgcggct gcttgcagcg gtgacggccg caggccccgt ggacctggct
     6061 gcggtggcgc aggcggcaca gaagtctccg gtgacgctgc aatgaaaggg gagatgcccg
     6121 caccgagcgg gcagggaagg ctagccgagt gacggaccgc gtgagacgca tcgccctgaa
     6181 gatcgatgtg gacaccgatc gtgggacgct ggagggggtc ccgcgacttg cgaccctgct
     6241 cgaacggctg gatgcgcggg caacttttct cttcagtctc gggccggatc agaccggccg
     6301 agcgctgcgt cgtgtgttcc ggcgcggttt cctcggcaag gtgcgcagaa cctcggtggt
     6361 gcaacactac ggggtaaaga cgctgctgta tgggaccttg ctacccggcc cgcacatcgg
     6421 tcgacggcgt ggtgcgctga tgcgcgaaat cgccaagagc ggattcgaag tgggtgtcca
     6481 tacctacaat cattcgaagt ggcaggacgg tgtggccact gccggggagt cgtggac
//
LOCUS       DASRHN010000203         7672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2047946, whole genome shotgun sequence.
ACCESSION   DASRHN010000203 DASRHN010000000
VERSION     DASRHN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047946"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFB37_07860"
     CDS             <1..432
                     /locus_tag="VFB37_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZO22408.1"
                     /translation="HHPFTSPANEDPAALRADPEGALANAYDMVLNGSEIGGGSVRIH
                     RQELQSTVFDLLGIEAAEAQKKFGFLLDALKYGAPPHGGIAFGLDRIAMLMAGADNIR
                     EVIAFPKTQTAADLMTEAPTEVSEAQLRELHIRVRTPPPAQ"
     gene            complement(451..4410)
                     /locus_tag="VFB37_07865"
     CDS             complement(451..4410)
                     /locus_tag="VFB37_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZO22409.1"
                     /translation="MRRTLRISAWSLGGLLGLLLLLIGALLIIGNTDSGRALIVRVTA
                     QLTKGHVQLSGIGGTFPGALDLDRLQLSDDRGPWLWAEHISLRWSPLALLTRHVEVDT
                     LHVGRLHIERAPLPSKPPKPSTTPSVPHSDLAHLSIDTLELGPALAGTAVSLVVEGNA
                     HLRSLQDAKAHVLAQRTGGFGNYEIEVQFDPQRVDASVKLREPPHGPLENILKVPGLG
                     ELSLLGTLSGPRNAAHIQLALAAGSIRGNVAGSIDLTKTSADLQYGLKAPAATPFPGL
                     SWQSVDLQGRWRGTIKAPFAEGHLEAFQLQTPGGTTLGALHANLAANAGLLSVKALVE
                     GLLIPGSQPKVFADAPLTLNATLHLSDPNRPLEITASHRLFTLKANATTVGEQTANVD
                     LRVPDVAPLAAVAGQKVGGSANVRAQIKRDATSTHLTADAEANIDGGTVAWASLLRGG
                     NARLQVAGELNEAQIAVEHLQLTGQAIALNASGTLGREAASPLDMRLDLSLPDLARLS
                     PSLAGTLRASGKVSGPSQSLTVSSDLTSTVSVHGSPVGTISAAVRAAGLPKTPKGTIE
                     AHGDLDGAPLLLSVSLQRTGDDLVHVLVQHADWKSAHIEGDLASGAELSQAKGTLRLR
                     MTRLADLDRILGSTLQGSIAGNLTLTPSAGQSRTELQLEARDFVSAGITTNAQLNAEG
                     PLNALHVKLAAQSPAVAGAPAGINSGGQLDVTARTLRLETLEGTYRGQTLKLLSPAKL
                     SFADGFSIEGLKLGAQQAVLTMDGRISPALAAHASLRQLKPDLINAFVPKLLASGNIQ
                     ADVQVQGSFAAPTGTIALDATGLRSASEVAGGLPATDLHAQAHLKGNTSEVDVKLVAG
                     SASQLALTGHAPLAADGALDLKLTGNLDVGLLNPMLEARGRHVAGQLVVDTTVTGAAA
                     NPEIGGTVKIAKASLRDYTQGTNLSDINGQLSGEHGTLRIENLTARAAPGDITIEGTI
                     GVLQPKIPVDVKVTARKAQPIANNIVTANLNADVTVKGTARERLDVEGTIHVNRADVE
                     IPSSFPPNVAVLDVRRPGQTPPPQNEKPLIIGLGVTVDAPRQILVKGRGLDAELGGEI
                     RIHGTTDSPAVSGGFELQRGFFTLASSKLTFSNGNVTFNGAGLKNRIDPTLDFTAQTQ
                     VTDATVTVKITGLADAPKIELSSTPDLPQDEILARVLFGVPAAQLTALQVVQIGAALA
                     TLSGGGSGAGFNPVAKIQKALGLDRLSVAGGSSNTGPAGAQQNTGASIEAGRYVSSRV
                     FVAVKESTTGTSQLAVDVDLTKHIKLQTRLGNGAAAQGTTPENDPGSSLGLAYQIEY"
     gene            complement(4410..6422)
                     /locus_tag="VFB37_07870"
     CDS             complement(4410..6422)
                     /locus_tag="VFB37_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZO22410.1"
                     /translation="MRIAAVMRQGLARVVVLGLALCGMPWAYGADPQPYKVEMASAGN
                     SELNSTLKATSELMTLRTSAPVGPYGLIGRARGDLDRLKTVLESYGYYQSYVAITIDA
                     LPLDDPGLGEELTARTKENDARVKISFSLGPVYHLRKIEVDGEVPKSAEDALGLSPGA
                     PAIAASVLAAGDRMLTALQDEGYAFAKVDTPIAYEDPANRVLDVRFHVVTGERVKIGE
                     IRVRGLKRIKESVVRKRLLVHTGEQYGGTKIETARKDLLALGVFSSVTVRVGSSPDSE
                     GRVPITFQIRERLQHAISLSGAYSSDLGGTTGVTWTDRNVSGGGDSLTLAASALNLGG
                     GTASNGIGYDTSAKYLVPDFHRRDQSLQVSLEALSQSLVAYDQKAVTAGVSLNRKLSS
                     IWSASVGFDVETERIIQQECVPLPPSNQLTSFNEYCHPGADGVCVLVAPDVFTPPQCV
                     SQTFHYTLLYLPISATYNSTGLISPLEDATHGVRITLTVTPTLSIGRPNTRFLITQGS
                     ISTYFDLEKLGLNKDPGRSVLALRAFGGAAGGASEFSLPPDQRFYAGGSGTIRGYRYQ
                     SVGPLFSDGNPVGGTAIQAGTAEYRQRIGTSFGTAVFVDAGQVSKNLNILNSTLRFGT
                     GAGVRYYTPIGPIRVDIAVPINKRPRTATFAGDDSFEVYIGLGQAF"
     gene            6574..7206
                     /locus_tag="VFB37_07875"
     CDS             6574..7206
                     /locus_tag="VFB37_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO22411.1"
                     /translation="MGEAVVYVHGLWMSGGESLLLRRRLSREFGLEVHPFRYAAAASG
                     MPEITARLQTFVRELKAPRLHFVGHSLGGLVIYRFLERFPQQPPGRAVFLGTPAVASR
                     AAVQASQIRLVASLMGKSVADELLRPRQRRWQADRPLGIIAGTQPLGFGQLLADFEED
                     CDGTIGVSETQLPGATDHITLPVSHLGMLVSAQVARATGAFLRDGRFTLR"
     gene            complement(7227..>7672)
                     /locus_tag="VFB37_07880"
     CDS             complement(7227..>7672)
                     /locus_tag="VFB37_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22412.1"
                     /translation="IATRLRAFTLFATHYFELTTLAAEVEGCANVHLDATEHGEGIVF
                     LHAVKDGPANRSYGLQVAQLAGVPHEVIAQARRYLEALESQRDRQSARNGGPVAAQAE
                     LPLQIPPPASDRLRQALEALDPDELSPKAALETLYRLRTLLADKS"
BASE COUNT         1248 a         2477 c         2548 g         1399 t
ORIGIN      
        1 catcacccgt tcacttcgcc tgcgaacgag gatccggcgg cgctgcgggc cgatccggaa
       61 ggcgcgctgg cgaatgccta cgacatggtg ctgaatggct cggagatcgg cggcggctcg
      121 gtgcgtattc atcgccagga gctgcagtcc acggtgttcg atctgctcgg catcgaggcc
      181 gcggaggccc aaaagaaatt cggattcctg ctcgatgcgc tcaagtacgg ggcgcctccg
      241 catggcggca tcgcctttgg gctcgacagg attgccatgc tgatggccgg cgccgacaac
      301 atccgagagg tcatcgcctt tccgaagacg caaacggcgg cggacctcat gaccgaagcg
      361 ccgaccgaag tgagcgaggc gcagctgcga gagttgcaca ttcgcgtgcg cactccgccg
      421 cccgctcagt agcgctcccg gcgctgccgc tcagtactcg atctggtaag ccagccccag
      481 gctgctgccc ggatcgtttt ccggcgtcgt cccctgtgcc gcggcaccat tccccaatcg
      541 cgtctgcagc ttgatgtgct tggtcagatc gacgtcgacg gcaagctgac tcgtgccggt
      601 ggtggattcc ttgaccgcga cgaacacccg gctggagaca tagcgtccgg cctcgatgct
      661 ggcgccggta ttctgctgag cgcccgcagg cccggtgttg ctcgagcctc ccgcgaccga
      721 cagccgatcc aacccgagcg ccttctggat cttcgccacg gggttgaagc cagcgccgct
      781 tcccccgcca ctcaatgtcg ccagcgccgc gccgatctga acgacctgca atgcggtcag
      841 ctgtgcggcg ggcacaccga acagcacgcg cgccaggatt tcatcctgcg gcaggtcggg
      901 tgtgctgctc agctcgatct tgggagcatc ggcgaggccg gtgatcttga cggtcaccgt
      961 cgcatcggtg acctgcgtct gcgcagtgaa gtcgagcgtc ggatcgatgc ggttcttgag
     1021 gccggcgccg ttgaatgtga cgttgccgtt ggagaaggtc agcttgctgc tcgcgagggt
     1081 gaagaatccg cgctgcagct cgaatccacc gctgaccgcc ggcgagtcgg tcgtgccgtg
     1141 gatgcggatc tcgccgccga gctcggcatc gaggccgcgc cccttgacca ggatctgccg
     1201 tggcgcatcc acggtcaccc cgagtccgat gatcagtggc ttctcgttct gcggcggcgg
     1261 agtctggcct ggccggcgca catcgagtac cgcgacgttg ggcgggaagc tgctcgggat
     1321 ctcgacgtcg gcgcgattga cgtggatggt gccctccaca tccagacgct cgcgcgcggt
     1381 gcccttcaca gtcacatccg cattcaggtt ggccgtgacg atgttgtttg cgatcggctg
     1441 cgccttgcgg gccgtcactt tcacatcgac cgggatcttc ggctgcagga ctccaatcgt
     1501 gccctcgatc gtgatgtctc ccggtgcggc gcgagccgtc agattctcga tccgtagcgt
     1561 cccatgctcg ccactgagct ggccgttgat gtcggacagg ttggtgccct gggtgtagtc
     1621 gcgcaggctg gctttcgcga tcttgaccgt gccgccgatc tccgggttgg ctgctgcccc
     1681 ggtgaccgtc gtgtcgacca cgagctggcc cgccacatgc cggccgcggg cctccaacat
     1741 cgggttcagc agacccacat cgaggtttcc ggtgagtttc aaatcaagcg caccgtcggc
     1801 agccaatgga gcatgtccgg tcagtgcaag ttgcgatgcg ctgccggcga caagcttcac
     1861 gtccacttca gaagtgttgc ctttgaggtg ggcctgcgcg tggaggtccg ttgcgggcag
     1921 tccgcccgcg acctcgctgg ctgagcgcag cccggtcgca tcgagcgcga tggtgcccgt
     1981 gggcgctgcg aaactgccct gtacctgcac gtctgcctgg atgttgccgc tcgccagcag
     2041 cttcggcaca aaggcgttga tgagatccgg cttgagttgg cgcagggagg catgcgcagc
     2101 gagcgccggg ctgatacggc cgtccatcgt caggaccgcc tgctgcgcac cgagcttcag
     2161 tccctcgatc gagaatccat ccgcgaacga cagtttcgcc ggagacagta gcttcaatgt
     2221 ctgcccgcga tacgtgccct ccagagtctc gaggcgcaaa gttcgcgccg tgacatcgag
     2281 ctgcccaccc gagttgatcc cggcgggcgc gcccgcaacg gcgggggact gggcggccag
     2341 cttcacgtgc agcgcattca ggggcccttc ggcgttgagc tgggcattgg tcgtgatgcc
     2401 tgcactgacg aagtcgcgcg cctcgagctg caactcggtt cgcgattggc ccgcggaggg
     2461 cgtgagcgtc agattgcctg cgatacttcc ctgcagggtg ctaccgagga tccgatcgag
     2521 atcggccagg cgggtcatcc gcaggcgcag cgtccccttc gcctggctca gttcggcgcc
     2581 gcttgccagg tcaccctcga tatgcgcgct cttccagtcg gcgtgctgga ccagcacgtg
     2641 caccagatca tcgcctgtcc tctgaaggga tacactgaga agcagcggtg cgccatccag
     2701 atcgccgtgc gcctcgatcg tgcctttggg cgttttcggc agccccgctg cgcgcaccgc
     2761 ggcggagatc gtgcccacag gggatccgtg caccgagact gtcgaagtca gatccgagga
     2821 gacggtgaga gactgactcg ggcctgacac cttgccggag gcccgcaatg ttccggccag
     2881 cgagggtgac agcctcgcca gatccggcag actcagatcg agtcgcatat ccagcggcga
     2941 ggccgcctcg cgcccgagcg tgccgctggc gttgagcgca atagcctgtc cggtcagctg
     3001 caagtgctcg acggcgatct gggcctcgtt cagctccccc gcgacctgca ggcgagcatt
     3061 cccgccacgc agcagactgg cccacgcgac ggtcccgcca tcgatgttcg cctccgcatc
     3121 ggcggtcagg tgcgtggagg tggcatcgcg tttgatttgc gcccgcacat tggcgctgcc
     3181 accgaccttc tggcccgcga ctgccgccaa gggcgcgaca tccggaactc gcagatccac
     3241 gttggctgtt tgctcgccca ccgtggtcgc gttcgctttc aaggtgaaca gccggtggga
     3301 ggcggtgatc tcgaggggcc ggttcggatc gctgagatgc agcgtcgcat tcagggtgag
     3361 tggcgcatcc gcaaatactt taggttggga gccggggatc agcagcccct caacgagggc
     3421 cttgacgctc aacagtcccg cgtttgccgc gaggttggcg tgcaacgcgc cgagagtcgt
     3481 cccgccggga gtctgcagct ggaaggcctc caaatgtccc tccgcgaagg gcgccttgat
     3541 cgtgccgcgc cagcgaccct gcagatcgac gctctgccag gacagtcccg gaaagggtgt
     3601 ggccgccggc gccttgagtc cgtactgcaa gtcagcggag gtcttggtca ggtcgatgct
     3661 gcctgcaacg ttgccgcgga tcgatccggc cgccagggcg agttggatat gggcggcatt
     3721 gcggggaccg ctgagggttc caagcagtga gagctcgccg aggccgggca ccttcaggat
     3781 gttctccagc ggaccatggg gaggctctct gagcttcacg gaagcatcga cgcgctgcgg
     3841 atcgaattgc acctcgatct cgtaattgcc gaagcctccc gtgcgctgcg cgaggacgtg
     3901 agccttcgca tcctgcagcg agcgcagatg ggcatttccc tcgacgacca gcgacacagc
     3961 ggtgcccgcc agagcgggcc cgagctcgag ggtgtcaatc gagaggtggg ccaggtccga
     4021 gtgggggacc gagggggtcg tgctgggctt gggaggcttg ctcggcagcg gagcgcgttc
     4081 gatatgcagg cggccgacgt gcagcgtgtc cacctcgaca tgacgcgtca gcagtgccag
     4141 cggtgaccag cgcagcgaga tatgctcggc ccataaccag gggccccggt cgtcactcaa
     4201 ttgcagccga tcgaggtcca gagcgccggg aaacgttccg ccgatccccg acagctgcac
     4261 gtgacccttg gtcagttgcg cggtcacccg cacgatgagc gcccggccgc tgtccgtatt
     4321 gccgatgatg agcagcgcgc cgatcaacag cagcagcagg cccagaaggc cgcccaggct
     4381 ccaggctgag atcctgagcg tgcggcgcat cagaaggcct gcccgagtcc gatgtagacc
     4441 tcgaacgagt catcgcccgc aaacgtcgcg gtgcggggcc gcttgttgat gggcacggca
     4501 atatccacgc ggatcggacc gatcggcgtg taatagcgca cccccgcgcc cgtcccgaag
     4561 cgcagcgtgg aattcagaat attcagattc ttgctgacct ggccggcgtc cacgaacacc
     4621 gcagtaccaa agctcgtgcc tatgcgctgt cggtactccg cggttcccgc ctggatagcg
     4681 gttccgccaa cgggattgcc atccgaaaac agcgggccga ccgattgata gcggtagccg
     4741 cggatcgtgc cgctgccgcc ggcatagaag cgctggtcgg gtgggagact gaactcgctc
     4801 gcgcccccgg ccgctccacc aaaggcccga agggccagga cgctccggcc cggatccttg
     4861 ttgaggccga gcttctcgag gtcgaagtaa gtcgagatgc tgccctgggt gatcaaaaag
     4921 cgggtgttgg ggcgacctat cgaaagcgtc ggcgtcacgg taagcgtgat gcgtacaccg
     4981 tgggttgcat cctcgagcgg cgagatcagc ccggtcgagt tgtacgtggc actgattggc
     5041 aaatacaaca gggtgtaatg aaaggtttgt gagacgcact gcggtggagt gaagacatcc
     5101 ggggccacta ggacgcacac gccgtccgca cccgggtggc aatactcgtt gaagcttgtg
     5161 agctgattcg atgggggcag cgggacgcat tcctgctgga tgatgcgctc ggtttcgaca
     5221 tcgaagccga cgctcgcgct ccagatactg gagagcttgc ggttcaagga aacgcccgcg
     5281 gtcaccgcct tctgatcgta ggccacgagc gattggctga gcgcctcgag ggagacctgc
     5341 aacgactgat cgcgccggtg gaaatcggga acgaggtatt tggcgctggt gtcatagccg
     5401 ataccgttgc tcgcggtgcc ccctccgaga ttcagggcgg aggcagccag cgtgagcgag
     5461 tcacctccgc ctgagacatt gcgatccgtc caggtcactc ccgtcgtgcc gcccagatca
     5521 ctggagtacg caccgctcag gctgatggcg tgttgcagac gctcgcgaat ctggaaggtg
     5581 atcggcaccc gcccttcgct gtcggggctg ctgccaaccc ggaccgtcac ggacgagaac
     5641 acgccgagcg ccagcagatc cttccgggct gtctcgatct tcgtgccgcc gtactgctcg
     5701 cccgtatgca ccagcagacg cttgcgcacg accgactcct tgatccgctt caggcctctg
     5761 acgcggattt cgccaatctt cacgcgctca ccggtcacta cgtgaaagcg cacatccagc
     5821 acgcggttcg cgggatcctc gtaggcgatg ggcgtgtcga ccttggcaaa cgcgtatccc
     5881 tcgtcctgca gcgccgtcag catccggtcg cccgctgcga gcacgctggc ggcgatcgcc
     5941 ggagcgccgg gggagaggcc gagagcgtcc tcggcgcttt tgggcacctc gccatcgact
     6001 tcgattttcc gcaggtgata aacaggcccg agactgaagc tgatcttgac gcgggcgtca
     6061 ttctccttcg ttcgggcggt cagctcttcc ccgagtcccg ggtcatcgag cggcagagcg
     6121 tcgatggtga tggcgacata gctctggtag tagccgtagc tttccaacac ggtcttcagg
     6181 cgatcgaggt ccccgcgagc ccggccgatg agcccatagg gcccgacggg tgcggaggtg
     6241 cgcaacgtca tgagctccga tgttgccttg agcgttgagt tgagctcgct gttaccggcg
     6301 gaagccattt cgaccttgta gggctgggga tcggcgccgt acgcccaggg catgccgcac
     6361 agggccagac ccagaactac aacgcgcgca agcccctggc gcatgacagc cgcgattcgc
     6421 aatgaccccc tccatcggat gagtgccggg cggcgatcgc ggcgggcgga cctgctactc
     6481 aattgcaccg cggcgccatg ctgcctgccg ccatttcttg gtaatgtcgg cctccgtaga
     6541 gtgttcccgg ccacgccagg attgtcgagc gatatggggg aagcggtagt ttacgtgcac
     6601 ggtttgtgga tgtccggggg cgagtcgctg ctgctgcgcc gccggctctc acgtgagttc
     6661 gggctcgagg tgcacccctt ccgctacgcc gccgcggcct ctggcatgcc cgagatcacc
     6721 gcgcggctgc agactttcgt ccgcgagctc aaggctccgc ggctgcattt cgtggggcac
     6781 agtctgggcg gcctggtgat ctatcgtttc ctcgagcgct tcccgcaaca gccacccggc
     6841 cgcgccgtct tcctcggcac ccccgcggtc gcctcgcgcg cggcggtcca ggcgagccag
     6901 atccggctgg tggcctcgct gatgggaaag tccgtggcgg acgagctttt gcggccgcgg
     6961 cagcgccgct ggcaagccga tcggccactc gggatcatcg ccggcaccca gccgctcggg
     7021 ttcggacaac ttcttgcgga tttcgaagaa gattgcgatg gcacgatcgg tgtcagcgag
     7081 acgcagctgc ccggcgctac ggaccacatc actttgccgg tgagtcatct gggcatgctc
     7141 gtttccgcgc aggttgcccg agccacgggc gctttcctgc gtgacggacg attcacgctg
     7201 agataggccg cccgcgcccg tcgcgctcag gatttgtccg caagtaacgt gcgcagcctg
     7261 tacagcgtct cgagtgccgc cttgggtgac agttcatccg ggtccagtgc ttcgagcgcc
     7321 tggcgcagac gatccgatgc cggcggtggg atctgcaggg gaagctccgc ctgcgctgcc
     7381 acggggccgc cgttgcgggc actttgccgg tcgcgttggg attccagcgc ctcgagataa
     7441 cgtcgcgcct gtgcgatgac ttcgtgcggg acgcccgcga gctgggccac ctgcaggcca
     7501 tagctgcgat tggccggacc atccttcacg gcgtgcagaa acacgatgcc ctcgccatgc
     7561 tcggtggcat cgaggtgcac gttcgcacaa ccctccacct cggcggccag cgtcgtcagc
     7621 tcgaagtagt gggtggcgaa cagggtaaag gctctgagac gggtggcgat gt
//
LOCUS       DASRHN010000204         5649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_400445, whole genome shotgun sequence.
ACCESSION   DASRHN010000204 DASRHN010000000
VERSION     DASRHN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400445"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..2213
                     /gene="flhA"
                     /locus_tag="VFB37_07885"
     CDS             129..2213
                     /gene="flhA"
                     /locus_tag="VFB37_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HZO22413.1"
                     /translation="MATAPSTTSLPPLRELIRTGIGVPVGVLAVLAMVVLPLPPMVLD
                     VLFTFNIALSLVIVMAVFYVARPVEFGVFPTILLLATLLRLALNVASTRVVLLNGHSG
                     TQAAGHVIESFGEFVIGGNFAVGVVVFIILTIINFVVVTKGAGRISEVSARFTLDAMP
                     GKQMAIDADLNAGLITQDEARARRIEVRAEADFYGSMDGASKFVRGDAIAGILILFIN
                     IIGGLAIGTVGHGMPIAAAGHTYTLLTIGDGLVTQIPALLLSTAVALIVTRMSRAQDM
                     GGELTRQLFGHPKALGVASGLLGTIGLVPGMPNLVFLLMSAVCGTGAYLLARKRAQPV
                     AAPAAPVAPTAPPESRELSWDDVQGVDLIGLEVGYRLVPLVDKRQSGDLLGRVRGVRR
                     KLSQELGFLVPAVHIRDNLDLAPNVYRINLSGVPVGEAVIYPERELAINPGRVFGPVQ
                     GITTRDPAFGMDAVWIEPATRDHAQTLGYTVVDPSTVIATHLSFIIQSHAHEILGHEE
                     AQHLLNTLAKTAPKLVEDLVPRVLPLSVVVRVLQGLLSERVPIRNIRTIIETLAEHAP
                     KTSDPVVLQGQVRVALGRQIVQDIAGVGSELPVITLEPELERLLQNSLAGNSTSPGLE
                     PGLAERLQRRVGESARRQEGAGEPAVLLVPPSLRHMLARFMRSSVPNLHVLAWNEIPD
                     SRKVRLVTAVNK"
     gene            2239..3966
                     /locus_tag="VFB37_07890"
     CDS             2239..3966
                     /locus_tag="VFB37_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22414.1"
                     /translation="MKIIRHTARDMRQAMRAVREQLGEDAVILSSKRTNDGVEVTAAV
                     DFDASTLEAGGFSNAPAAAPAAPAVLAAAAREIAAPAPQAIASPAAQVTPSPAAPPAT
                     PAPRALSRRRLLASRSALATSRAAAESSTATADEASSLLDARPTQSYDSDCISAAARE
                     AVAAHARALSQPPVAAQQAPSLATPAEGHTDAMERAVAAAPTLSAAPARSRTELPVYR
                     SDEVIERARASSLAPFGAQEGTSDSGMSAPAPELEELTAPRVEVPAGSPTAEAMGNEL
                     KSLRRMLETQLAHLAWNDLTRRAPIRTEILRELTEIGITHDLSEHLVRQLPEDTDLTF
                     ARRFTIAGLSQYLRVTGDRWLDEGGRVAFAGATGVGKTTTLAKVAVRWVLRHGPRDLA
                     LVAADPVRIGAQDQLRSLAQLLGVAVYSPDQFENLPSLLSTLTERLVLIDTPGSSARD
                     AQLGSRLAVLASSASKLETALVLSASTQTGAVEAAVKRFAPANPSSCVLTKLDEAASL
                     GGVLSILIRARLPVSYVSEGQRVPEDLRPARALELVSEAVRLAKASGAAADEDLLRRR
                     FGKIAHGIT"
     gene            3953..4834
                     /locus_tag="VFB37_07895"
     CDS             3953..4834
                     /locus_tag="VFB37_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HZO22415.1"
                     /translation="MASPELNIIRNAKLEGLAQPVRVIAVTGGKGGVGKTSVSVNLAT
                     ALAASGKRVVLLDGDLGLANADVFLGLSPRYTLAHVISGERTLDEVLISAPQGFHVVP
                     AASGAADMANMSEAEHLGLVQAFSSLAAKVDVLIVDTAAGIAHSVLQFSQASQHVVVV
                     VCDEPASLTDAYALVKVLSRNHGVTRFQVLANQIRVPGSGAELFRRFELVASRFLEVV
                     LEFAGEIPEDEYLRRSVREQRPVFDAYPSCPASRAFKKLAARAETWPVPSGPRGNIEF
                     FVERLVRRNAPRLEAVR"
     gene            4831..5559
                     /locus_tag="VFB37_07900"
     CDS             4831..5559
                     /locus_tag="VFB37_07900"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HZO22416.1"
                     /translation="MNAVSAYSAGRASGDADRLVLQHAELVKRIAYHLAGRLPPSVEV
                     DDLIQAGMLGLLEAAENYAADRGASFETYAGIRIRGAMIDALRKLDWAPRSVHRKARA
                     AAAVVQEIERRTGRDARDAEIAEGMGISLEEYHAIVQDAASCRLASLDDATAATAAGP
                     ADPFREAADERFRTALARAIEGLPERERLVMSLYYSDGLNLKEIGGVLKVTESRVCQL
                     HGQALVRLKARLAEWRDTAVPDQA"
BASE COUNT          943 a         1871 c         1851 g          984 t
ORIGIN      
        1 tcgctttatg tcgcggtcgc ccaggtcctc acctacatct accagctcaa gaccgcgcgc
       61 agccagggca tgaacccgcc gcagccgccc gatatcgatc ccaacattga tcagacccgg
      121 cattgatcat ggccacggca ccctcaacga cgtccctgcc tccgctgcgc gagctgattc
      181 gcaccgggat cggtgtaccc gtcggcgtgc tcgccgtgct cgccatggtc gttctgccgc
      241 tgcccccgat ggttctggac gtgctcttca cgttcaacat cgcgctgtcg ctcgtgatcg
      301 tgatggcagt gttctacgtg gcgcgcccag tcgaattcgg agtcttcccg acgatcctgc
      361 tgctggcgac attgctgcgt ctggcgctga acgtcgcttc cacgcgcgtg gtgctcctga
      421 acgggcacag cggcacgcag gcggccggac atgtcatcga gtcgttcggc gaattcgtga
      481 tcggcggcaa cttcgccgtc ggcgtggtcg tgttcatcat tttgacgatc atcaacttcg
      541 tggtggtcac caaaggcgca ggccgcatct ccgaggtgag cgcccgtttc accttggatg
      601 cgatgcccgg caagcagatg gcgatcgatg ccgacctgaa tgccggcctg atcacgcagg
      661 atgaggcccg ggcgcgccgc atcgaggtgc gagccgaagc cgacttctac ggctccatgg
      721 atggtgcgag caaattcgtg cgcggcgatg cgatcgccgg catcctcatc ctgttcatca
      781 acatcatcgg cgggcttgcc atcggcacgg tcggacacgg tatgccgatc gcggcagcgg
      841 gccataccta cacgctgctg accatcgggg acggcctggt cacccagatt ccggccctgt
      901 tgctctcgac cgccgtcgcc ctgatcgtga cccgcatgtc ccgcgcgcag gacatgggtg
      961 gtgagctcac ccggcagctc ttcggccatc cgaaggcctt gggcgtcgcc tccggattgc
     1021 ttgggacgat cggcctcgta cccgggatgc ccaacctggt tttcctgctc atgtcggccg
     1081 tatgtggcac gggcgcgtac ctgctcgcca gaaagcgcgc gcagccggtc gccgcgccgg
     1141 cggcgcccgt ggctccgacc gcgccccccg agagccgcga gctctcctgg gatgacgtgc
     1201 agggtgtgga tctcatcgga ctggaagtcg gctatcggct ggtgcccctg gttgacaagc
     1261 ggcagagcgg tgacctgctg ggccgcgtcc gcggcgtgcg gcgcaagctc tcacaggagc
     1321 tcgggttcct cgtcccggcg gttcatatcc gcgacaacct cgacctggca cccaatgtct
     1381 accgcatcaa cctctccggt gtgccggtgg gagaagcagt catctatccc gaacgcgagc
     1441 tcgcgatcaa tcccggccgc gtgttcggac cggtgcaggg catcacgacc cgcgatcccg
     1501 ccttcggcat ggatgcggtg tggatcgagc ctgcaacgcg cgaccatgca cagactctcg
     1561 gctacacggt cgtggatccg agcacggtca ttgccacgca cctgagcttc atcatccagt
     1621 ctcatgcgca cgagattctc ggccatgagg aagcgcaaca tcttctcaac acgctggcca
     1681 agacggctcc gaagctcgtg gaggatctcg tgccgcgcgt gctgccgctc tcggtcgtgg
     1741 tccgcgttct gcaggggttg ctcagcgagc gcgtgccgat ccgcaacatc cgcaccatca
     1801 tcgagaccct cgccgagcac gcacccaaga cttcggatcc ggtcgtgctg cagggccagg
     1861 tgcgcgtggc actcggccgc cagatcgttc aggacatcgc cggcgtaggc agcgaattac
     1921 cagtcatcac gctcgagccg gagctcgagc gcctgctgca gaattcgctc gcgggcaaca
     1981 gcacgagccc gggactcgag ccgggactcg ccgagcgttt gcagcgacgc gtgggcgaat
     2041 cggctcgccg ccaggaagga gccggcgagc ctgccgtgct gctcgtgcca ccttcgctgc
     2101 gacacatgct cgctcgcttc atgcgctcga gtgtgccgaa cctgcatgtg ctcgcgtgga
     2161 acgagatccc ggacagccgc aaggttcgtc tggtcacggc agtaaacaag tgagtgggat
     2221 cgtgcgtgga gtagccagat gaaaataatc cgacacacgg cgcgcgacat gcgccaggcc
     2281 atgcgcgccg tgcgcgagca gctcggcgag gatgccgtca tcctctcctc gaaacgaacc
     2341 aacgacggcg tggaagtcac ggccgccgtg gacttcgatg cctccacgct ggaagccggt
     2401 ggcttcagca acgcgccggc tgccgcacct gctgcgccag cggtcctcgc agccgctgcg
     2461 cgtgagattg ccgcgcccgc ccctcaggca atcgcctcac ccgcggcaca ggtcacccct
     2521 tcgccggccg caccccccgc gactcctgct cctcgagcac tcagcaggcg gcgcttgctc
     2581 gcaagcagat cagctttggc gacttcgcgt gcagcggcgg agtcgagcac agcaacagcg
     2641 gatgaggctt cctcgctgct ggatgcccgg cccacccaat cctacgattc cgattgcatc
     2701 tcggcggcag cgcgcgaagc ggtggccgcg cacgcccgcg cgctctcaca gccgccggtc
     2761 gccgcccagc aagctccttc ccttgcaact ccggccgagg gccacacaga tgcaatggag
     2821 cgcgccgtgg ctgcggcacc cacactctcg gctgcaccgg ctcgctcacg cacggagctt
     2881 ccggtctatc gctcagacga ggtcatcgag cgggctcgcg cttcctcgct ggcgccgttc
     2941 ggcgcgcagg aggggacctc cgatagcggc atgtccgctc ccgcacccga gctggaggag
     3001 ctgaccgccc cccgggtcga agtccccgcc ggctcgccca ccgcggaagc catgggcaac
     3061 gagctcaagt cgctgcgtcg gatgctcgag acacaacttg cgcacctggc atggaacgat
     3121 ctgactcgcc gcgctccgat ccggaccgag atcctgcgag agctgaccga gatcggcatc
     3181 acgcatgacc tgtctgagca cctggtgcgg caacttccgg aagataccga tctcaccttc
     3241 gctcgccgct tcacgattgc cggcctgtcg caatatctgc gcgtgaccgg agaccgatgg
     3301 ctcgacgagg gcggcagagt tgccttcgcg ggggcgaccg gcgtcggcaa gacaacgacg
     3361 ctggccaagg tggccgtgcg ttgggtactg cggcacggac cccgcgatct ggcgctcgtc
     3421 gccgcagatc cggtgcggat cggcgcacag gatcagctgc gctcactcgc gcagctgctg
     3481 ggggtagctg tgtactcacc cgaccagttc gagaatctgc cctcgctgct gtccacgttg
     3541 accgagcggc tggtgctcat cgatacgccg ggctcgagtg cgcgcgatgc gcagctcggc
     3601 agccgcctgg cggtgcttgc cagctcggcc tcaaagctcg aaaccgcgct ggtgctgtcg
     3661 gcaagcacgc agaccggagc ggtggaggcg gccgtgaagc gctttgcgcc ggcgaacccc
     3721 tcctcctgtg ttctgacgaa actcgatgag gccgccagtc ttggcggggt gctctcgatc
     3781 ctcatccgcg caaggctgcc ggtctcgtac gtgagcgagg gacagcgcgt gccggaggac
     3841 ctgcggccgg cgcgggcact ggaactggta tcggaagcag tccggctcgc caaggcgagc
     3901 ggggcggcgg ccgatgaaga tcttctgcgc agacgttttg gaaagatcgc ccatggcatc
     3961 acctgagctc aacatcatcc gcaacgcgaa gctcgagggg ctcgcccagc ccgtgcgcgt
     4021 gatcgccgtc acgggcggca agggcggcgt cggcaagacg agcgtctcgg tcaacctcgc
     4081 cacagcgctg gccgccagtg gaaagcgcgt cgtgctcctg gatggagacc ttgggctcgc
     4141 caacgctgat gtctttctgg ggctgtcgcc gcgttacacg ctggcacatg tcatcagcgg
     4201 cgaacgtacc ctggatgagg tactgatcag cgcgcctcag ggctttcacg tggttccagc
     4261 cgcctcgggc gcggcagaca tggccaacat gagtgaggcc gagcacctgg gtctcgtgca
     4321 ggctttcagt agtttggctg caaaggtgga cgttctcatc gtcgatacgg ccgccggaat
     4381 cgctcatagt gtgctgcagt tctcacaagc atcgcagcac gtggttgtcg tggtatgcga
     4441 tgagccggct tcgttgacgg atgcctacgc tctggtcaaa gtgttgagcc gcaatcacgg
     4501 ggtcacccga ttccaggtgc tcgccaatca gatccgagtg ccgggttcgg gcgcggagct
     4561 gtttcggcgc ttcgagctcg tcgcctcacg attcctcgag gtagtgctgg agttcgcagg
     4621 cgaaatcccc gaagacgagt accttcggcg ctcggtaagg gagcaacgac ccgttttcga
     4681 cgcctatccc tcgtgtccag cctcacgtgc attcaagaag ctggcagcgc gcgccgagac
     4741 gtggcctgtg ccgtccggcc cgcgcggcaa catcgagttc ttcgtggagc gactcgtgcg
     4801 ccgtaacgcg ccgcgactgg aagcggttcg atgaacgcag tgagtgccta cagtgccggg
     4861 cgtgccagcg gggatgctga ccgtctggtg ttgcagcacg cggagctggt gaagcggatc
     4921 gcctaccacc tggccggcag actgccgccc tcggtggagg tcgatgatct gatccaggcg
     4981 ggcatgctcg gacttctgga ggcagcggag aactatgccg ccgaccgcgg ggcgagcttc
     5041 gagacctatg cgggaatccg catccgtgga gcgatgatcg atgcgctgcg caagctcgat
     5101 tgggcgccgc gatcggtgca ccgcaaggcg cgtgccgccg ccgccgtggt gcaggagatc
     5161 gagcggcgta cgggacggga tgcgcgcgat gcggagatcg ctgaaggcat gggcatctcg
     5221 ctcgaggaat atcacgcgat cgtgcaggac gctgcgagct gccggctcgc cagcctcgat
     5281 gacgccacgg ccgcgacggc cgccggtccc gcggacccat tccgcgaagc ggcggacgag
     5341 aggttcagga cggcgctggc ccgagcgatc gagggcctgc ccgagcgcga gcggttggtc
     5401 atgtcgcttt actacagcga cgggctcaac ctgaaggaga ttggtggagt gttgaaggta
     5461 accgaatcgc gcgtctgcca gctccacggc caggcgctgg tacgactgaa agcacgactt
     5521 gcggagtggc gggacactgc cgtcccggac caggcgtgaa tgcgagtgag caggagagcg
     5581 aaccgtgagt aaagacctga gatttctggt ggtcgatgat ttctcgacga tgcgtcgcat
     5641 catcaagaa
//
LOCUS       DASRHN010000205         5330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1056660, whole genome shotgun sequence.
ACCESSION   DASRHN010000205 DASRHN010000000
VERSION     DASRHN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056660"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFB37_07905"
     CDS             complement(<1..307)
                     /locus_tag="VFB37_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZO22417.1"
                     /translation="MSAAPGAGFRSGFAALVGRPNVGKSTLLNALVGQKLSIVTPRPQ
                     TTRHRIVGISHLPEAQVAFVDTPGLHEHGSRALNRVMNRTAATSLADSDLVVLVLEAL
                     "
     gene            complement(304..960)
                     /gene="rnc"
                     /locus_tag="VFB37_07910"
     CDS             complement(304..960)
                     /gene="rnc"
                     /locus_tag="VFB37_07910"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZO22418.1"
                     /translation="MREHLGYEAQDPQLFAAALTHRSVPGPNNERLEFLGDAVLNLVI
                     ARHLYQAFPQGSEGDLSRLRARLVSGQPLAEVAAQVGLGEALQLGSGELKTGGFRRQS
                     ILSDAFEAVCGALFLDGGLAAAGKVIEQLFEHRIAALPAPASLKDAKTRLQEYLQSQG
                     HALPRYSVERTEGEAHEQTFHVLCEVSELGRSAHGSGTSRRRAEQEAAARLLEEIEPT
                     "
     gene            complement(1024..1404)
                     /locus_tag="VFB37_07915"
     CDS             complement(1024..1404)
                     /locus_tag="VFB37_07915"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HZO22419.1"
                     /translation="MRTKQRGVTMIGWIFLLIPLAIVVYAGIRLAPVYLNYMKVARTL
                     DQVKSEFKPGDAGNRQAILIAIEKHFNIESVDYPTVKDLKISRDGQSWIVEAAYDDQA
                     PLFSNMFILVTFDKQVALGGGGAE"
     gene            complement(1434..2531)
                     /gene="lepB"
                     /locus_tag="VFB37_07920"
     CDS             complement(1434..2531)
                     /gene="lepB"
                     /locus_tag="VFB37_07920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZO22420.1"
                     /translation="MLMQIAVFLSWLAVPVGLIVVLDDWFIRPQRLIAASPQPPNDPW
                     LLRIAYLSLPILVAAAVLRLLVAERLDFSAVLLGITVVTGLAWAVDVAVLRRRRAAAA
                     QAAGKDPTTIPEPGTVDYARSFFPVALVVLVLRAFVFEPYRIPSDSMMPTLLDGDFII
                     VSKYAYGLRLPVLNRKIVSVGEPQRGDVVVFRYPLDPSVNYIKRLVGLPGDHVEVRSD
                     RLTINGKTVPFKVTRPYNDGCYVNMQLAEEHLGLHDHQALLCPVPLEVTAGPPAGCNR
                     SESRGYFCNASTPPGADGGVFGQAAPVVEDVPPGMYFMMGDNRDNSEDGRVWGFVPEA
                     NLVGKATRIWFNWDLKRSGGPKWSRIGSAIQ"
     gene            complement(2548..4350)
                     /gene="lepA"
                     /locus_tag="VFB37_07925"
     CDS             complement(2548..4350)
                     /gene="lepA"
                     /locus_tag="VFB37_07925"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZO22421.1"
                     /translation="MLLTRNFSIIAHVDHGKSTLADRFIQLCGGLQEREMQEQVLDSM
                     ELERERGITIKAQSVTLYYDAADGQRYQLNMIDTPGHVDFSYEVSRSLAACDGALLVV
                     DASQGVEAQSVANCYTAIEQGIEVLPVINKIDLPSADPGRVRKEIEEIIGIDAADAVQ
                     VSAKTGEGVAELLEMLIRRIPAPKGDPLAPLQALIIDSWFDNYVGVVSLVRVMNGGLK
                     TGEKFRVMSTGRSHTADKLGRFMPKSVALRELATGDVGFVVAGIKEIDGAPVGDTITL
                     EHRPSPQPLPGFKQIKPRVFAGLFPVNSDDYEGFRDALSKLRLNDSALHFEPEVSGAL
                     GFGFRCGFLGLLHMDIVQERLEREYNLELVTSAPTVVYEVLRTDGTVVYVDNPAKLPV
                     ANEVEEIREPIITASILVPPDHVGAVLKLCTDKRGRQKKMQYLGTQVSLQYELPLAEV
                     VLDFFDRLKSVSRGYASFDYEFSHFEAGPLVKLDILINGDKVDALSVIVHRENAYHRG
                     RELVDKMQELIPRQMFEVAVQAAIGGSIIARASVKALRKNVLAKCYGGDVSRKRKLLE
                     KQKEGKKRMKQLGHVEIPQEAFLAVLQVGRKPQA"
     gene            4540..4767
                     /locus_tag="VFB37_07930"
     CDS             4540..4767
                     /locus_tag="VFB37_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22422.1"
                     /translation="MEFLDVEAVTADHGAIEQQNGDIESVPAQKLRIAVDVHDLDGRE
                     ALAATKRIQLSQHLIAEIASVAMHHGQFKGG"
     gene            complement(4815..>5330)
                     /locus_tag="VFB37_07935"
     CDS             complement(4815..>5330)
                     /locus_tag="VFB37_07935"
                     /inference="COORDINATES: protein motif:HMM:NF028497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HZO22423.1"
                     /translation="AMPLKSQLCDARGHVIEQIVFADLSLPAHIPDSSFKPEVPTAGF
                     HWLRNDSPPVKESPENATMAWNAMMLPPGFRMAARSAQIMPGSEGPVSHLVFTDGLAS
                     VSVFVESRSAPADQQTVMEESLHVGSSSVFSTIMGSRQVTAVGEVPPATVRSIASSFA
                     SQAPVLTPAHH"
BASE COUNT          967 a         1754 c         1692 g          917 t
ORIGIN      
        1 tcagtgcttc gaggaccagc acaacgaggt ccgagtccgc gagcgaggtg gccgcggtgc
       61 ggttcatgac tctgttgagt gcgcggctgc catgttcgtg cagacccggc gtatcgacga
      121 aagcgacctg ggcctcgggc aggtgcgaga tcccgacgat gcggtggcgt gtcgtctgtg
      181 gcctgggtgt gacgatgctg agcttctgcc cgaccagcgc gttgagcaga gtggacttgc
      241 ccacgttcgg gcgcccgacg agggctgcga aaccgctgcg gaatccggcg cccggtgcgg
      301 cgctcatgtg ggctcgatct cctcgaggag gcgcgctgcg gcctcctgct cggcacgccg
      361 ccggctggtg ccgctgccgt gcgcgctgcg gcctaactcg gacacctcgc acaggacgtg
      421 gaaggtctgc tcatgcgcct cgccctcggt ccgttcaaca ctgtagcgcg gcaacgcatg
      481 gccctgcgac tgcagatact cctgcagtcg cgtctttgca tccttgagcg acgccggagc
      541 ggggagcgcg gcgatccggt gctcgaacag ctgctcgatc accttcccgg cggccgcaag
      601 gccaccatcc agaaacagcg caccgcagac cgcctcgaac gcatcactga gaatggactg
      661 gcggcgaaag ccgcccgtct tgagctcgcc ggagcccagc tgcagcgcct cgcccaggcc
      721 cacttgcgcg gcgacctcgg cgagcggctg gccgctcacc agccgcgcac gcagccggct
      781 cagatctcct tccgaaccct gcggaaacgc ttggtaaagg tgccgggcaa tcaccaggtt
      841 gaggaccgcg tcgccgagga attccaggcg ctcgttgttg gggccgggca cgcttcgatg
      901 ggtgagcgcg gcagcgaaga gctgcggatc ctgggcttcg tagccaagat gctcgcgcac
      961 ccaacgagcc ggccaatccc cgctcgtcat ggggacacac gcgagccatc cgccaccggc
     1021 aaactactcc gcgccgcccc cgccgagtgc cacctgcttg tcgaacgtga cgaggatgaa
     1081 catgttcgag aacagcggcg cctgatcgtc gtaggccgct tccacgatcc atgactgccc
     1141 gtcccggctg atcttgagat ccttcacggt cgggtaatcg acactctcga tgttgaaatg
     1201 cttttcgatc gcaatcagga ttgcctggcg gttgccggca tcccccggtt tgaactcgct
     1261 cttcacctgg tcgagcgtgc gcgcgacctt catgtagttg agatagaccg gcgccaggcg
     1321 gatcccggca tacactacga tggccaacgg gatcagcaga aagatccagc cgatcatcgt
     1381 caccccgcgt tgcttcgtac gcatcgatgc ctcccgcatt gcgcccggat ggctcactgg
     1441 atcgccgaac cgatccggct ccacttcggg ccgccgctgc gcttgagatc ccaattgaac
     1501 cagattcgtg tcgctttgcc gaccaggttg gcctcgggga cgaagcccca gacccgtccg
     1561 tcctcactgt tgtcccggtt gtcgcccatc atgaagtaca tgcccggcgg cacgtcctcg
     1621 accacgggcg ctgcctgtcc gaacacgccg ccatccgccc cgggtggggt gctggcattg
     1681 caaaaataac ctcgcgactc actccggttg caaccggccg ggggaccggc ggtcacctcc
     1741 agcggcaccg ggcagagcag cgcctggtga tcatgcagcc ccaggtgctc ctctgcgagc
     1801 tgcatgttca cgtagcagcc gtcgttgtag ggacgtgtca ccttgaaagg cacggtctta
     1861 ccgttgatcg tcagccggtc gctgcggacc tccacatggt cgcccggaag gcccaccagg
     1921 cgcttgatgt agttcacgga aggatcgagc ggatagcgga acacgaccac atctccgcgc
     1981 tgcggctccc cgacggagac gatcttgcgg ttgaggaccg gcaggcgcag tccataggcg
     2041 tacttgctga cgatgatgaa gtccccatcg agcagggtgg gcatcatcga gtccgaaggg
     2101 atccggtaag gctcgaagac gaaggcccgc aggaccagga cgaccagggc tacggggaag
     2161 aaactgcgcg cgtagtccac ggtccccggc tcggggatgg tcgtcggatc cttgcccgcg
     2221 gcctgcgccg cagctgcccg gcggcggcgc agcacggcca catccaccgc ccaggcaagc
     2281 cccgtgacga ccgtgatacc gagcagcacg gcgctgaagt cgagccgctc ggcgaccagc
     2341 agccgcagca ctgcggcggc gaccaggatc ggcaggctca ggtaggcgat ccgcaggagc
     2401 caggggtcgt tgggcggctg cggcgaagcg gcaatcagcc gttgcggccg gatgaaccag
     2461 tcatcgagca cgacgatcaa cccgacaggc acggcaagcc aggacaagaa cactgcaatc
     2521 tgcatcagca tcggcatcgc tctcccttca agcctgcggc ttgcggccga cctgcagcac
     2581 cgccaggaat gcctcctgcg gaatctccac gtgtccgagc tgcttcatgc gcttcttgcc
     2641 ctctttctgc ttctccagga gtttgcgttt gcggctgacg tcgccgccat agcacttggc
     2701 cagcacgttc ttgcgaagtg ccttcaccga ggcgcgcgca atgatcgacc cgccgatcgc
     2761 cgcctgcacc gccacttcga acatctgccg ggggatgagc tcctgcatct tgtccacgag
     2821 ctcgcgcccg cggtgatacg cgttctcacg atgcacgatg accgacaggg catcgacctt
     2881 gtcgccgttg atcaggatat cgagcttgac cagcgggccg gcctcgaagt gactgaactc
     2941 gtagtcgaac gaggcatagc cgcgactcac cgacttcaat cgatcgaaga aatccagcac
     3001 gacttccgcc agcggcagct cgtactgcag tgacacctgg gtgccgaggt actgcatttt
     3061 cttctgccgg ccgcgcttgt cggtgcaaag cttcaacacc gcaccgacgt gatcgggggg
     3121 cacgagaatg ctggcggtga tgatcggctc gcgaatctcc tcgacctcgt tcgcgacagg
     3181 cagcttggcg ggattgtcca catacacgac cgtcccgtcc gtgcgcagca cttcgtagac
     3241 caccgtcggg gcgctcgtca cgagctcgag gttgtactcg cgctcgagcc gctcctggac
     3301 gatgtccata tgcagcaggc cgagaaaccc gcagcggaat ccgaatccca gtgcgccgga
     3361 gacttccggc tcgaagtgca gcgccgaatc gttcaatcga agcttcgaca aggcatcgcg
     3421 aaagccctcg tagtcatcgg aattgaccgg gaagaggcct gcgaacacgc gcggcttgat
     3481 ctgcttgaag cctggcaagg gctgcgggct tggccgatgc tcgagcgtga tggtgtcgcc
     3541 gacgggcgcg ccgtcgatct ccttgatgcc cgccaccacg aagcccacat ccccggtggc
     3601 cagctcgcgc agagcgaccg acttcggcat gaagcggccg agcttgtccg cggtatggct
     3661 gcgaccggtg gacatgacgc ggaacttctc accggtcttg aggccgccgt tcatgaccct
     3721 caccagagag acgacgccca cgtaattgtc gaaccacgag tcgatgatca gcgcctgtag
     3781 aggcgcaagc ggatcaccct tgggtgccgg gatgcgccgg atgagcatct cgaggagctc
     3841 cgccacgccc tcgcccgtct tggcgctgac ctggaccgca tcggcggcat cgatgccgat
     3901 gatctcctcg atttccttgc gcacgcgacc cggatccgcc gagggcaggt cgatcttgtt
     3961 gatgaccggc agcacttcga tcccctgctc gatcgccgtg tagcagttgg cgacgctttg
     4021 agcctccacg ccctgcgagg catcgaccac gagcagcgcg ccatcgcagg ccgccaaaga
     4081 gcgcgagact tcgtacgaga agtccacgtg ccccggcgtg tcgatcatgt tgagctggta
     4141 gcgctgcccg tctgcggcgt cgtagtacaa ggtgacgctt tgcgccttga tcgtgatgcc
     4201 acgctcacgc tcgagctcca tcgagtcgag cacctgctcc tgcatttccc gctcctgcag
     4261 cccgccgcat agctggatga agcggtcggc cagggtggat ttgccatgat caacgtgagc
     4321 gatgatggag aaattgcgtg tgagcagcat gaatccacag tctgtcgcgt gtgccgcgcc
     4381 aggaccctgc ggcgcgcgga ttatagttta ttggcctaaa gcgcctacgg cgatttaggc
     4441 ggagtctgcg taccaatcct ttcggggccc ggcaaaagac gtggctttgc cgggtaggac
     4501 cccgggcccc caaccgaccg ccagctagcg cagcagccgt tggagttcct cgacgtcgag
     4561 gcggtgacgg cagaccacgg tgccatcgag cagcaaaacg gggacattga gtccgtaccg
     4621 gcgcagaagc tccggatcgc cgtcgacgtc cacgacctcg atgggcggga ggctctggcg
     4681 gcgaccaagc gtatccagct cagtcagcat ctcatcgcag agatcgcatc cgtggcgatg
     4741 catcacggtc aatttaaagg aggctgacaa gggttgagga cctccgcaga agccattccg
     4801 tcctgcgcgg gaggtcagtg atgcgcgggg gtaagaaccg gcgcctgcga cgcgaaagag
     4861 ctcgcgatcg agcgcaccgt cgccggtggg acctccccca ccgcagtgac ctgccgcgat
     4921 cccatgatgg tcgagaagac cgacgaggag ccgacgtgca gcgactcctc catcacggtc
     4981 tgctggtcgg ccggggccga gcgggactcg acgaaaacgg acaccgaggc cagtccatcg
     5041 gtgaatacca ggtgcgagac cggcccctcg gatccaggca tgatctgcgc cgaacgcgct
     5101 gccatccgga accccggcgg cagcatcatg gcgttccatg ccatcgtggc attctccggc
     5161 gactccttca caggcgggga gtcgttgcgc agccagtgga atcccgcagt ggggacttcc
     5221 ggcttgaatg aggagtccgg aatgtgagcc gggagcgaca ggtcggcgaa gacgatctgc
     5281 tctatcacgt gtccgcgggc atcgcagagc tggctcttca ggggcatggc
//
LOCUS       DASRHN010000206         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_400698, whole genome shotgun sequence.
ACCESSION   DASRHN010000206 DASRHN010000000
VERSION     DASRHN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400698"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VFB37_07940"
     CDS             complement(<1..1727)
                     /locus_tag="VFB37_07940"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22424.1"
                     /translation="MRDAALLGESTAPAPVLDCRNLAVSYFYGGRQTVAVVDFNLTLR
                     PGEAHGLVGESGCGKSTVALAIMRHLAAGGRITAGEILFEGRDLLRLDREALRRVRGA
                     GIAMVYQEPMASLNPSLTIARQLTEVPVCHEGVSQHEALTRARSMLDMVQLADAQRIM
                     RSYPHQISGGQQQRVVIAMALLSRPKVLLLDEPTTALDVTVEAGIVDLIREIAARFGM
                     AMLYISHNLGLVRETCDRMTVMYAGEAVEVGAVEDVFRAMRHPYTRGLFAALPSLAHD
                     KRTRPLRAIPGQLPSPGSRPAGCVFGPRCGDFVAGQCDTTRVALRATGTAADSPSHLS
                     RCLRIAEIDWDAAPSPSSQPPALGTGRGATLLRVRDLTKHYRHVIANDRLTFDVSRGE
                     TVAIVGESGSGKSTFARVAMGLAAASAGSIQFEDLELAQLLVTHRSRPTLRALQMIFQ
                     NPFETLNPQHTVGAQIERVIRKFKVEHQEWRVRSRMLELLELVKLPRAVAQQFPGELS
                     GGQKQRVAVARAFAANPELVIADEPLSALDASVQTAVTELLLDLQREQGTAVVFISHD
                     LAVVRHLAER"
     gene            complement(1727..2752)
                     /locus_tag="VFB37_07945"
     CDS             complement(1727..2752)
                     /locus_tag="VFB37_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22425.1"
                     /translation="MTNQDQATLLAPSLVRILSPARGVALLLALIWFPLAIFVLYRAE
                     LGEQARYVLIPAIPAVLILYAWALSSWRQSRVALFGVTLVCFWLLIAVTVPFLPLADP
                     DKPVAPYVVPGMVRHGTLFLLGSDTRGRDVLSRTLWGCQRVLVWGVTATLMAYGVGVA
                     FGLLSGYLRGWWDEIVSFASNLLLSFPVMVLLILVLNVLGQSGFNIIIAVTCSSAPII
                     MRIVRGLVLEARERDYVLAAQIRGEHPLWIMLVELLPNVRGPLIVDACLRLGYTTVAI
                     TTLTFLGLGLQPPDPDWGLMIKEAANAALLWKFSYMLIVPALSVTSLILGFNLIADGL
                     REMSWRA"
     gene            complement(2749..3699)
                     /locus_tag="VFB37_07950"
     CDS             complement(2749..3699)
                     /locus_tag="VFB37_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22426.1"
                     /translation="MNVLLLRRVIQMLLIMLTVAALLFVIFDSEQFRHKAAVAELGAL
                     GLATLSEADYQGWLQRKGLNRPAPVRFVRWLKDIAHGDLGQSSEKDVAVSTLLKESLG
                     HTAVLAFFVFLIMIPLAIALGVLAGMETGSMLDRLISVVSVITTSTPQIATAVVLTVV
                     FALGLRWFPSKSAMTEGFSFRELVLPLLTILIYDVGYLARMTRASMAEVMGSQYIRTA
                     LLKGLPYRQVVVRHALRNALVVPVTLICLELNGILSQVVVVEVFFQYSGFGRMLFDAA
                     NYGDIQVLQAATLVAVFVAVMSQLLSDVAYRFLNPRMRFA"
     gene            complement(3711..>5438)
                     /locus_tag="VFB37_07955"
     CDS             complement(3711..>5438)
                     /locus_tag="VFB37_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO22427.1"
                     /translation="SVRPYRKTRLTRDRPHPLLAKLRRQLQQGVMDRREFIRFASLLG
                     AGAATAYAMAGLNPPAWAEESLPFPTPDAGAKRGGKLRVGQMVTRMVDPATYSWNEMA
                     NQTRPILDYLTLVGPDNVVRPMLAESWQASDDLRTWVLHIRGGVMWHNGEQLTAEHVA
                     WNIRRWSDPTLGSSNLGLSTFAALTQFTGEHDVAGKPVRRALPGGVEVLDGRSLRLNL
                     AQPVLSVPEDCGEFCTLILHPSFTPPFSKHPLGTGPYTLSELRVADRCILKRISQTTD
                     GKPFRYWGGEVFLEEIHFYNYEQENQAVALASGSVDLIYELTLEQLELARAIPDAQIL
                     SVETAQTLCCRMQVDKPPYDDIRVRRAILKAADNAAIKTLVLGDFGSVGWNFHVSPVQ
                     PDYAPLEPMVRDVAGARELLREAGYERGLDLEIVVGNNDGPWHQAVCEALRDQLREAG
                     IHLAVHVVPPTKFWEIWNKVPFGATSWAHRPLGTMTLAQAYRTGASWNETHYSDPDFD
                     QALAQAEATIDILERRKIMHRVEQILRDAALMIQPLWRPYFILATARVHGYRPQPARQ
                     MQLTRVWMS"
BASE COUNT         1090 a         1756 c         1710 g          882 t
ORIGIN      
        1 cgctcggcga ggtggcgcac gaccgccaga tcgtgactga tgaatacgac ggctgtcccc
       61 tgctcccgct gcaaatcgag cagcagctcg gtcaccgcgg tctgaacgga tgcgtcgaga
      121 gccgagagag gctcatcggc aatcacaagc tccggattgg cggcgaacgc acgtgcaacc
      181 gcgaccctct gcttctgccc tcccgagagc tctcccggaa attgttgtgc cacggcgcgc
      241 ggcagtttca ccagctcgag caactcgagc atgcgcgagc ggacccgcca ctcctgatgc
      301 tctactttga acttgcggat gacacgctcg atctgcgctc cgacggtatg ctgaggattg
      361 agcgtctcga aaggattctg gaagatcatc tgcaatgctc gcagcgtggg ccggctgcgg
      421 tgtgtgacga gcagctgagc cagctcgagg tcctcgaact ggatcgagcc cgcgctcgca
      481 gctgcgagcc ccatcgccac acgcgcaaag gtcgacttgc ccgagccgga ctcgccgacg
      541 attgccacgg tctcaccccg gctcacatcg aacgtcaggc gatcattggc gatcacgtgc
      601 cgatagtgct tggtgagatc gcggacacgc agcagcgttg cgcccctgcc ggttcccagc
      661 gcaggcggct gcgagctcgg cgacggtgca gcatcccagt cgatctccgc gatgcgcaga
      721 cacctggata gatgggatgg cgaatcggcc gccgtgcccg tcgctcgcag cgctacacga
      781 gtagtgtcac attgcccggc gacaaagtcg ccgcagcgcg ggccaaacac gcaccccgca
      841 ggccgtgagc ccggcgaagg cagctggccg ggaatcgccc tcagcggccg agtgcgcttg
      901 tcgtgcgcca gcgaggggag cgctgcgaag aggccgcggg tgtacggatg ccgcatcgct
      961 cgaaatacgt cctctacggc gcccacctcg accgcctcgc ccgcatacat gacggtcatg
     1021 cgatcacagg tttcacgcac gagacccaga ttgtgggaga tgtagagcat ggccatgccg
     1081 aagcgcgcgg cgatctcccg gatcaggtct acgatgcctg cttcgaccgt cacatccagc
     1141 gctgtcgtgg gctcatcgag cagcagcact ttcggcctcg acagcagtgc catcgcgatg
     1201 acgacccgct gttgctgccc gccggagatc tgatgcggat aggaccgcat gatgcgctgc
     1261 gcatcggcca gctgaaccat gtccagcatc gaacgcgctc gcgtgagggc ctcgtgctgt
     1321 gacacgccct cgtggcaaac cggcacctca gtgagctgcc tcgcgatggt cagcgaggga
     1381 ttgagcgatg ccatcggctc ctgatacacc atggcaatgc ccgcaccgcg tactcgccgc
     1441 aacgcctccc ggtcgagcct caacaggtca cgcccctcga acaggatctc ccctgccgtg
     1501 atgcgtccgc cagccgcgag atgccgcatg atcgcgagcg cgacggtgga cttgccgcag
     1561 ccggattccc ccaccaggcc atgagcctct ccggggcgca gcgtgagatt gaaatctacc
     1621 accgccacgg tctgtcggcc gccataaaag taggacactg cgagattgcg gcagtccagg
     1681 acgggcgccg gggccgtgct ttccccgagg agcgcagcgt cgcgcatcag gctctccagc
     1741 tcatttcgcg cagcccgtcg gcaatgaggt tgaacccgag tatcaggctc gtcaccgaga
     1801 gcgccgggac gatcagcatg tacgagaact tccagagcag cgcggcgttg gcggcctcct
     1861 tgatcatgag tccccagtcg ggatccggcg gctgcaggcc gaggccgagg aaagtgagcg
     1921 tggtgatcgc taccgtcgta tagcccagcc gcaggcaggc atccacgatc aatggcccgc
     1981 gcacgttcgg cagcagctcg accagcatga tccacagcgg gtgctcaccg cggatctgcg
     2041 ctgcgagaac gtaatcccgc tcccgggcct ccagcaccag gccccgcacg atccgcatga
     2101 tgatcggggc cgatgaacag gtgaccgcaa tgatgatgtt gaatccgctc tgcccgagta
     2161 cgttgagcac gaggatcagg agcaccatga ctgggaagga gaggagcagg ttgcttgcga
     2221 acgagacaat ctcgtcccac cagcctcgca ggtagcccga cagcagcccg aaggctacgc
     2281 cgaccccgta ggccatcagg gtggccgtca cgccccacac cagcacgcgc tgacagcccc
     2341 acagcgtgcg cgacaggaca tctctgccgc gtgtatcgga gccgagcaga aacagcgtcc
     2401 cgtgccgaac cattccgggc accacgtagg gcgctacggg cttgtccgga tcggcaagcg
     2461 gcaggaacgg tacagtcacc gcgatcagca gccagaagca cacgagcgtg acaccgaaga
     2521 gggccacacg cgattgccgc caggaactca gcgcccaggc gtacagaatg agtacggcag
     2581 gaatggcggg gatcagcacg tagcgcgcct gctcgccgag ctcagcgcga tagaggacga
     2641 agatcgcgag cgggaaccag atcagggcga gcagcagcgc gacaccgcga gccggactca
     2701 ggatgcgcac cagtgatggc gcaagcagcg ttgcctgatc ctgattcgtc atgcgaagcg
     2761 catccgtgga ttcaggaatc gataggcgac atccgagagc agttgcgaca tcacggccac
     2821 gaacaccgcg accagggtgg cggcctgcag cacctggata tccccatagt tcgctgcatc
     2881 gaagagcatg cggccaaagc ccgagtactg gaagaacacc tccacgacca cgacctgcga
     2941 caaaatgccg ttgagctcca ggcagatcag tgtgacaggg accaccaatg cgttgcgcag
     3001 cgcatgcctc acaacgactt gtcgataagg cagccccttc agaagggcgg tgcggatata
     3061 ttgcgagccc atcacttccg ccatcgaggc gcgggtcatc ctggctagat agccgacgtc
     3121 gtaaatcagg atggtcaaca gaggcaacac cagctcgcgg aaggagaagc cctccgtcat
     3181 ggcagatttg ctcggaaacc accgcaaacc caatgcaaag acgacggtca acacgaccgc
     3241 cgtggcgatc tgtggcgtcg atgtcgtaat gacggacacg acggaaatga ggcgatcgag
     3301 catcgagccg gtttccatcc ccgcgagcac gcccagcgca atggccaggg ggatcatgat
     3361 gaggaatacg aaaaaggcca ggactgccgt atgccccagg ctctccttca gcagcgtgga
     3421 cacggcgaca tccttctcgc tggactgacc cagatcgccg tgcgcgatgt ccttcagcca
     3481 ccggacaaag cgcactggag ccgggcggtt caatcccttg cgttgcaacc atccctggta
     3541 gtccgcttcg gacaaggtgg ccagaccgag ggcaccgagc tcggctactg cggctttgtg
     3601 ccggaactgc tcgctgtcga agatcacgaa caggagcgct gcgacggtca gcatgatcaa
     3661 cagcatctgg atcacgcggc gaaggagcag cacgttcatc acgagcgagc tcagctcatc
     3721 cagacgcggg tcagctgcat ctggcgcgcc ggctgcggcc ggtagccatg cacccgggcg
     3781 gtggccagaa tgaaatacgg cctccacagt ggctggatca tcagcgccgc gtcgcgcagg
     3841 atctgctcga cccgatgcat gatcttcctg cgctcgagga tgtcgatggt ggcctccgcc
     3901 tgagcgagtg cctggtcgaa atccggatcg ctgtaatgcg tctcgttcca ggacgcgccc
     3961 gtgcgatagg cctgggcgag ggtcatggtg ccaagcggac ggtgcgccca tgaggtcgca
     4021 ccgaacggca ctttgttcca gatctcccag aatttggtcg gcggcacgac atggacggcg
     4081 aggtggatac ccgcttcgcg caactgatcg cgcagtgctt cgcacacggc ctggtgccag
     4141 gggccatcgt tgtttccgac gacgatctcc aggtcgagac cgcgctcgta gcctgcctcg
     4201 cgcagcagtt cgcgcgcgcc ggcaacgtcc cggaccatcg gctcgagggg cgcgtagtcc
     4261 ggttgtacag gtgacacgtg aaagttccag cccacggaac cgaaatcgcc cagcaccagt
     4321 gtcttgatgg cggcattgtc ggccgccttg aggatggcac ggcgtacgcg gatgtcgtcg
     4381 tagggcggct tgtccacctg catgcgacag cacagtgttt gcgcggtctc cactgacagg
     4441 atctgcgcat ccggaatggc gcgggcgagc tccagctgct ccagcgtcaa ctcgtagatc
     4501 aggtcaacac tgccggaggc gagtgccacg gcctgattct cctgctcata attgtagaag
     4561 tggatctcct cgagaaacac ttcaccgccc cagtagcgga atggcttgcc atcggttgtc
     4621 tggctgatgc gtttgaggat gcatcggtcg gcgacccgca actcggaaag cgtgtagggt
     4681 ccggtgccga ggggatgctt cgagaaaggt ggcgtgaagg aaggatgcag gatcagcgtg
     4741 cagaactcgc cgcagtcctc cggaacagac aaaacaggct gagcaagatt caggcgcaag
     4801 ctccgcccat ccaacacctc cacccctccg ggcaacgccc gccggaccgg cttgcccgca
     4861 acatcgtgct caccagtgaa ctgcgtgagg gcggcgaacg tggacaggcc caggttcgag
     4921 gagcccaggg tcggatccga ccatctgcgg atattccagg cgacgtgctc cgcggtcagc
     4981 tgttcgccgt tgtgccacat cacaccgccg cggatgtgca gcacccacgt ccgcagatcg
     5041 tccgaggcct gccatgactc ggccagcatg ggccgcacga cattgtccgg ccccaccaga
     5101 gtcaggtagt ccaggatggg gcgcgtctgg ttggccattt cattccagct gtaggtcgcc
     5161 ggatccacca tgcgcgtcac catctggccg acgcgcagct tgccgccgcg ctttgcgccg
     5221 gcatccggtg taggaaacgg caaagattct tcggcccagg ccggcggatt cagacccgcc
     5281 atggcgtaag cggtggcggc tcctgcgccg agcagactag cgaaccttat gaattcccgt
     5341 cgatccatga caccctgctg cagctgccgg cgcagcttcg cgagcaacgg atggggtcga
     5401 tctcgggtca acctggtttt cctgtaagga cggacgct
//
LOCUS       DASRHN010000207        14972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2048981, whole genome shotgun sequence.
ACCESSION   DASRHN010000207 DASRHN010000000
VERSION     DASRHN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14972
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048981"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="VFB37_07960"
     CDS             complement(<1..1716)
                     /locus_tag="VFB37_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZO22428.1"
                     /translation="MNLAGKNSNRQLVIVGIGTVLTIVMGAVLIAGFRLATHMRSNVT
                     GLQSASMLQTYPGAIAQQLNALRDRLESRAYSGQALADLRVTVQRFDYELGKLGAADT
                     AQSPQLEQARLLWRQYGPVVNPVIDFDGQPYVDSDEGGSTFSKSGRVHYANVKRAQLF
                     ATENQKALEGQLAAAATNLQQATSEAANKLRALLSMGVFAALVLAAAAAYFQATRSRH
                     ERAAREAQEQTRDILKTVREGFFLLDADYQIGSVWSEALTRMFGRSDFAGLSFEELLK
                     GLVPQATLATAMKYIKLLWGDRAHENLMKSINPLGQLEITMENTHGGKETRYLQFDFH
                     RVMGPKGIKHVLCAVGDITSSVLLAKELQESQENANQQLDMMLGMMHVDPLQLSTFLD
                     STETGLQLINAILKEPARTDGEFRKKLNGLFRELHSIKGEASALNLLSIAHRVHTLED
                     MVSELKKRAELSGNDFLPMVLKLDDLLAHLRSVREMSTRLSTLKHSGPAPAPEATSLR
                     TPRLGASAEQRAPGRSGEDLSPTLHSMAERLAHDHHKRFKLTMTGLADVPGAYVGTVK
                     DCVIQM"
     gene            complement(1741..3954)
                     /locus_tag="VFB37_07965"
     CDS             complement(1741..3954)
                     /locus_tag="VFB37_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZO22429.1"
                     /translation="MASPASTASRPSEATPDSASSTTATNSRPAVEVTAVTSRDDLLL
                     ELGEALSGQASVHPVESMGEALPHLSGGKRAHVLVIDTRDITDIRTEVQRVDAAAPQA
                     IVLVLAAADSEKEVAGAVKGSKVFAVLPIPLDKRKSAAVLDAAVAEARDRKAARSGES
                     AQGAEHSHAPASGLAHSHGSAHSSVTQAHGLDQAMAVSVEPFRPHSRSDSLSDGDWKA
                     RLPLIAGAVIAVLALAAGGYFLFGRSSNQAVPHPAAAKNTPASAAAPAAGNKAAEETL
                     AAPTVETSLVQGKVDELLEKARQAMRERRYSEPAGDNALLYYRSAAAADPANGEAGDG
                     LQRVAGVLSNRFEDAMSGGRFDEAGQALANLKLATPKDSSLAQLELRLTTAQVAKALA
                     DGNPDRAAALVRQAQQSNAVPADQITRWRADISRRQEEGKIQRLATLVSDRTRDGKLI
                     DPAGDSARDYLQQLREQAPSNSTTQRLMHELNGAFLRKAREASLANRSAESDRWLSEA
                     KAGGATAAEIGAYQRDLTGARQRAAAAESERLGQLVRDRLREGRLTDPAQDSAAFYLG
                     QLQASDGSSAGSAVLARELAGKLLDRARSSAQSGRPVDADLAQAKHFGADPKDILAVQ
                     QIQSAPRGAAGGAQRSPADLAALAANMKRLRYVAPEYPQKALQLNATGAVTVEYTVDV
                     KGDTRDVHVVESSPPGMFDRSAIAAIKRWHYQPAVVDGTPVEVPIRTAIRFELPK"
     gene            4171..6159
                     /locus_tag="VFB37_07970"
     CDS             4171..6159
                     /locus_tag="VFB37_07970"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZO22430.1"
                     /translation="MRVGRIVAFTAGTIVVLLVIVVVALLLFVHPDSYRGRIEEAVRQ
                     QTGRELTIGGKLELRIFPWLAFSVQGLSLGNPPEFGSQPFLTVQQASIGVRPLPMLLS
                     RRLEVSRISVDGLSAHLVKRGTTNNWHDLTESKSTAAPPPRTASGSGHAISVGRIDLR
                     HCVVILSDETAHSTTTLSIDELHSGEVALGSETGGLTLEDFELKGSLLRAAAGTPAAK
                     PLPFSLRAPELALDPRTQALAPARIEAHAGELSVIVSVSGQKTPAGQVLSGRIEVPKV
                     SARRVMDSLGIAAPVTRDSHVLSVLALTSGYRFEPNRLELSGLDLTLDDTHVQGTAAI
                     SDLAQDAWTFNLSVDAINLDRYRPPAPAGGAAAQKNPPPPGKHPEAPTPLPIETLRKL
                     NLHGTLQVGSATVAGLMFTGMRLPVSAQSGAIHLGPAQALVYGGTYNGDIALDARPAD
                     AQLSLNQHLHGTDIGALLKAGFDTTRLSGHADVNMAVSGSGNTNAAIMSSLGGKIDAS
                     VLQGALNGIDIAYELQSANALLKREVPPKSSSPARTQFNILQTSGTLEHGILHTDVLR
                     IETNFLKVSGTGTLNVVTEAIDYRLAAALSQGAPMSHSGAGPRSTALEVPLLVTGTAA
                     NPTVRPDLEALAKGQLRQQGGDLVRKQLGDKLKGLLNH"
     gene            6485..8449
                     /locus_tag="VFB37_07975"
     CDS             6485..8449
                     /locus_tag="VFB37_07975"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22431.1"
                     /translation="MQPLSEDSGSQSKANLTFIESLSSFSRLHRAQRWEGTFGDFLAS
                     ILPANPVAMARTSHQYIWDMLRWHGRASQPDLPDGANARELFKRELFGIDEPLSRVVD
                     YFKGAAAGSDVGRRLLLLLGPPSGGKSTLAIMLKRGLEEYSRTDEGAIYAIKSSPLRE
                     NPLNLVPASLRPEFREHYGVEIQGELSPWAREVVEREYDGDFVRVPVERVFLAESSRT
                     GIGTYAPHDPTTADIADLVGSVDLAKVAQIGDEGDPRAWSWSGAVYAASRGMLEMIEI
                     LKVKREFLYLLLTMTQEKNVKVSRFPLIHLDETILAHTNLAEFHKFLQEKENEALLDR
                     MVIIKVPYALSYREESRIYQKLVSGAPAFRNVHLDPHVLNLASVFAILTRLTKTEREG
                     LDLTKKLRLYAGEAVEGVPESEASRLHAESADEGLTGVSPRFVINALSNAITRGNTHS
                     LTSMELLLAIKDAIESDARIDAKQKKAWVDHLVTARKEFYNRWVKEDVHRAMFASFEE
                     EAQQLLEKYLDEVEASLDHRQVTDPITSESRPADERFLRSVEEKIKVSDSGKMSFRQE
                     VVRKAMVAFKAGEKFKLASHARMREAIEQYLFEERRDVLRLVTSTARPDEEARQKISV
                     VQQRLVSEYGYDEHSAKEALSYVTTLLAQE"
     gene            8547..9719
                     /locus_tag="VFB37_07980"
     CDS             8547..9719
                     /locus_tag="VFB37_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HZO22432.1"
                     /translation="MAESSDKPGSPDAAKWYELFSRGARDWLRHDEKVRESVRKHLPQ
                     IVSGADVINEGARTVRVPVRMLEHYHFRLRRPEEQQGAGQGKAKPGDVFSDPSQQRGP
                     GQKGSGGQDEGEVQLLLEFKIDDIVDWLWEEMQLPNLKARVGPSEESDWIREGWDRRG
                     ARSRLDRRRSFKEMVKRRGAPGATPSFIDEDLRYRQLARRRQPAVHAVVFFMLDVSGS
                     MSDRDRKLAKTFFFWVVQGLRREYRSLETVFVAHTTEAWEFNESEFFQVTGTGGTVSS
                     TGFNKVREIMDARYNPGRCNIYLFYASDGDNSVSDSTLARESLSAITGDACFTGYVEV
                     SSGLSRQLATETGRLFAELQTAGCAAGSYALNDFDDVWGAVRHFFTAESRALEAPG"
     gene            9716..11077
                     /locus_tag="VFB37_07985"
     CDS             9716..11077
                     /locus_tag="VFB37_07985"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HZO22433.1"
                     /translation="MTEDWHDYTQRIEELAVRSGLSFHPVDFEAVPDSFMMEIAVYGL
                     PVRMPHWSFGVRYIYQLIQRHMGHSRLFEVVFPGNPGHAYLAASNGLAENILVTAHVL
                     GHADFSRNNLLFRRCQGQVSEHIVEHAATHARQIQQAIEQYGAQRVEVVLDAALALEQ
                     HIDVDQSLRRERYPEYEAENKAIVDDDFRKRFAALDPAAAAGALEIRKRAPVPPHPER
                     DLLWFVANYAPEMESWERDIFLAVREESFYFYPVFATQIMNEGWASYWHARLLREAEF
                     VPQQAYVDAIKCHSDVVRPVAADQQVALTINPYHLGFAMWEKIVEKEGLEGARGIMQQ
                     DDDFGFIRNHLTRELADELGLFRFNARSDGQVKVLEADMSALQEAVLGPKYNFGAPSV
                     SAAHVRNDGTLELMHDSASDGRGLDLERSRRVIDYVQRVWRRPITLTTVNQAGSTLEL
                     TAP"
     gene            complement(11259..11435)
                     /locus_tag="VFB37_07990"
     CDS             complement(11259..11435)
                     /locus_tag="VFB37_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22434.1"
                     /translation="MTEPTPEARERHRKKVQQQLDEALAESFPASDPVSIVTSQNEED
                     WTSDPDEAEETPKK"
     gene            complement(11642..14554)
                     /locus_tag="VFB37_07995"
     CDS             complement(11642..14554)
                     /locus_tag="VFB37_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22435.1"
                     /translation="MKIQIGNGGISFVACVLLAGVAPQVALSQAAPAAADDKIQEVIV
                     TGSRIAQSEAQREQPLSVISNDAIERTGLADVGNLLQQLTTGGSALNAKFNSSGNFGY
                     PPDGGGIGAGSTQVDLRNLDSKRTLVLVDGIRWVNESSASGVSGSADLNTIPLPIIDH
                     IEVLEDGASSIYGSDAIAGVVNIITKKKMDGVELNGYTGEYGKGGQTRQGSLSVGGSS
                     DKFSGMFVASYYNQDAISSSKWWQSDVPEPFAGLRAGSSATPQGRYTFCDPRVAVPNL
                     GSCTPDQGNFYDMTLNNGTTTPVWNPLNPFGAGSTYHAWSNADRFNFAPFNLLLTPQT
                     RKSIFTNLSYQASDNVEVYVKGLYNNRKSENQAAPEPIFVGPYAGTGGLADTINISAK
                     NPYNPFGIDLDAASNFGWVTRRPLEAGPRIFDQNVDTYYFGTGLRGNFNMLGSEYRWD
                     VNYVNSANKANQTFTGGYNIGNIAIALGDPAVCAQVPNCVPLDLFGGQGRPITPTMLK
                     FIEAVQIDRSEQDLRIFSANITGHPFSIQDRPVGIAIGAEHRLYKGDFSPDPLRTEGL
                     SQDSLAFPVDASFRVNEAYGEVSAPLLETLGASAAIRYSDYSNFGSTTTYKGGLRWQP
                     IQDFGLRGTYSTGFRAPNLGELFGLTQFGATLVDPCGPTGTIIVTPGNHSALANACRA
                     QGVPNNFQQANTQITTFTGGNADLRPEKSDSFTAGFVYNAGWAEGMMHTARLTLETTY
                     YNHKIKGAIEAEDIQALLNSCLDAGGTDPLLCAPFTRGAGGQLRPPKNFLANLGHITT
                     DGEDLKLNWLTEPLPIGHFSAAVQATRVNSYKAVDTLGLVAQRQVGIEVSNSAIPRYR
                     MNAQLGYGIADVEFTWTVRYLSAVTEACSNAAEVGVPGCETTTDVHTLHAVTYNDAQL
                     AWNNAFWLKDLTLEAGVNNLFGVNPPVCFTCTLNGYDAGTYDLPGAFWNVRAKYKF"
BASE COUNT         2644 a         4721 c         4860 g         2747 t
ORIGIN      
        1 catctggatc acgcagtcct tcacggtccc gacatacgcg cccggcacat ccgcgagccc
       61 agtcatcgtg agcttgaagc gcttgtggtg atcgtgggcc aggcgctcgg ccatggaatg
      121 cagggtgggc gacagatcct cgccggatcg gcccggtgcc cgctgctcgg cgctcgcccc
      181 gaggcgcggc gtgcgcaggg aggttgcttc gggtgccggc gccgggcccg aatgcttgag
      241 ggtgctgagg cgggtggaca tctcgcgcac gctgcgcaga tgggccagca ggtcatcgag
      301 cttgagcacc atgggcagga agtcattgcc cgacagctcg gcgcgcttct tcagctcgct
      361 caccatgtcc tcgagagtgt gcacgcgatg cgcgatgctg agcaggttga gcgccgaggc
      421 ctcgcccttg atggagtgca gctcgcggaa cagcccgttc agcttcttgc ggaactcgcc
      481 gtcggtgcgc gccggctcct tgaggatcgc attgatgagc tgcagtcccg tctcggtgct
      541 gtccaggaag gtgctgagct gcagcggatc gacgtgcatc atgccgagca tcatgtcgag
      601 ctgctggttg gcattctcct gcgactcctg cagctccttc gccagcagca cgctggaggt
      661 gatatcgccg acggcgcaga gcacgtgttt gatgcccttg gggcccatga cgcggtggaa
      721 gtcgaactgc agatagcgcg tttccttgcc gccgtgcgtg ttctccatcg tgatctcgag
      781 ctgcccgagg gggttgatgc tcttcatcag gttctcgtgc gcccggtcgc cccacagcag
      841 cttgatgtac ttcatcgcgg tggcgagcgt cgcctggggc accaggccct tcagcagctc
      901 ctcgaaggac aaacccgcga agtcactgcg gccgaacatg cgggtcaggg cctcggacca
      961 gaccgagccg atctgatagt cggcatcgag caggaagaat ccctcgcgta ccgtcttgag
     1021 gatgtcgcgg gtctgctcct gcgcttcccg ggcagcgcgc tcgtgacgcg agcgggtggc
     1081 ctggaagtag gccgcagccg cggcaagcac cagcgccgcg aacacgccca tggacagcag
     1141 cgcgcgcagc ttgttggcgg cctccgaagt ggcctgctgc agattggttg cggcagcggc
     1201 gagctgaccc tcgagcgctt tctgattctc cgtcgcgaag agctgcgcgc gcttcacgtt
     1261 tgcataatgg acacggccgg acttggagaa ggtgcttccc ccctcatccg agtccacata
     1321 aggctggccg tcgaaatcga tcacgggatt gaccaccgga ccgtactggc gccacagcag
     1381 gcgcgcctgc tcgagctggg gcgactgcgc ggtatcggcc gccccgagct tgccgagctc
     1441 gtaatcgaag cgctgcaccg tcacgcgcag gtccgcgagg gcctggccgg agtaggcgcg
     1501 cgactcgagt cgatcccgca gagcgttcag ctgctgtgcg atcgcgccag ggtaggtctg
     1561 cagcatgctg gccgactgca ggcccgtgac gttcgagcgc atgtgagtcg ccaggcgaaa
     1621 gcccgcgatg agcacggcgc ccatgacaat ggtcagcacc gtaccgatgc cgacgatcac
     1681 gagctggcgg ttgctattct tgcctgccag attcattaat gacttctcct gcggcccccg
     1741 ttacttggga agctcgaatc ggatcgcggt gcggatcggc acctcgacgg gcgtgccatc
     1801 cacgaccgcg ggctggtagt gccagcgctt gatcgccgcg atggccgagc gatcgaacat
     1861 acccggcggc gacgactcca cgacgtgcac atcgcgggtg tcacccttca catcgactgt
     1921 gtactcgact gtcacggcgc ccgtcgcatt cagctgcagg gccttctgcg gatactcggg
     1981 cgcaacatag cgcaagcgct tcatgtttgc cgcaagagca gcgagatcgg cagggctgcg
     2041 ctgcgcaccg ccagccgcgc cgcgcggcgc actctggatc tgttgcaccg ccaggatgtc
     2101 cttcggatcc gcgccgaaat gcttcgcctg agcgaggtct gcatctaccg gccggccact
     2161 ctgggcagaa gatcgagcgc gatcgagcag cttgccggcc agctcgcgcg cgagcactgc
     2221 agagcccgca gagctgccat cgctggcttg cagctgacct aagtagaagg ccgcactgtc
     2281 ctgagccgga tcagtcagcc gcccctcgcg caaacggtcc cgaacaagct gtcccaaccg
     2341 ctcggattcc gcagccgccg ctctctgccg cgcgcccgtg aggtcccgtt gatacgcccc
     2401 gatctccgca gccgtcgctc cgcctgcctt ggcttcgctg agccagcggt ccgactccgc
     2461 tgagcggttg gcgaggctgg cctcgcgcgc cttgcgcagg aaggctccat tcagctcatg
     2521 catcagccgc tgcgtggtcg agttgctggg cgcctgctcg cgcaactgct gcagatagtc
     2581 cctcgcgctg tcgccggccg ggtcgatcag tttgccgtcc cgcgtgcggt cactgaccag
     2641 tgtcgccagg cgctggatct ttccttcttc ctgtcgccgg gagatatcgg cgcgccagcg
     2701 ggtgatctga tcggcgggca cggcattcga ttgctgcgcc tggcgcacca gcgcagccgc
     2761 cctgtcgggg ttgccgtctg ccagcgcctt ggcgacctgg gcggtcgtga ggcgcagctc
     2821 gagctgcgcc aggctggaat ccttcggtgt ggccagcttc aagttggcga gggcctgacc
     2881 ggcctcgtcg aagcggccgc cgctcattgc atcctcgaag cgattgctca gcacgcctgc
     2941 cacccgttgc agtccgtccc cggcttcccc gttggcgggg tcggcggcgg ccgcagagcg
     3001 gtaataaagg agtgcgttat cgccagccgg ttcagagtac cgacgctcgc gcatcgcctg
     3061 tctggctttc tccagcagct catccacctt gccctgcacg agggaagtct ccaccgtcgg
     3121 tgcggcgagg gtctcctcag cggccttgtt gccggcggct ggcgcggccg cagaagcagg
     3181 ggtgtttttc gccgcagccg ggtgtgggac agcctggttc gagctccttc cgaacaggaa
     3241 gtacccgccc gcagccaacg cgagcaccgc gatgactgcc cctgcgatca gcggcaatcg
     3301 ggccttccaa tcgccatcgg acagggagtc ggatcggctg tgcggacgga atggctcgac
     3361 gctgacggcc atcgcctgat ccaagccatg ggcctgagtg acggacgagt gggccgatcc
     3421 atgggagtgc gcaagacccg atgctggagc gtgcgagtgc tctgcaccct gcgcggactc
     3481 gccgctgcgt gcggcttttc ggtcccgcgc ttcggccacg gccgcatcga gcacggcggc
     3541 cgacttgcgc ttgtccagcg ggatgggcag aacggcgaaa accttcgatc ccttgacggc
     3601 gcccgccacc tccttctcgg agtcggcagc ggccaacacc aacacaatgg cctggggcgc
     3661 cgcggcgtcc acccgctgaa cctcggtccg gatgtcggtg atatcccgcg tgtcgatcac
     3721 caggacgtgc gcacgcttgc cgccggagag gtgcggcaga gcctccccca tggattcgac
     3781 ggggtgtacc gaggcctggc cgctgagcgc ttcgcctaac tcgaggagca gatcgtcacg
     3841 gctcgtgacc gcggtgacct ccacggcggg acggctgttg gtcgccgtgg tggaagaggc
     3901 ggaatccggt gtggcttcag agggccggga ggcggtcgaa gcggggcttg ccacggtaag
     3961 tctccatcgt tggtcattgc agagagacgc ctggcgcctc ctgcccgatc gttgccggag
     4021 tatgcggagc tctatgtcga ggcagcatag ttagctgctg cggcaccaca aggctacagt
     4081 tcacaataat gatagggtac cgtcgtaatc tgacgacgct ccggagtcac atcctgtgca
     4141 ctatctcacg ctttcgccag gggtccagtc atgcgtgtag gccggatcgt cgctttcacc
     4201 gcggggacga tcgtcgtcct gctcgtcatc gtcgttgtgg cgctgttgct gttcgtgcat
     4261 ccggacagtt atcgcgggcg catcgaggag gccgtgcggc agcagaccgg ccgcgagctg
     4321 accatcggcg gcaagcttga gctaaggata tttccctggc tggccttttc ggtacagggc
     4381 ctgagcctcg gaaatccacc cgagtttggc tcgcagccct tcctgacagt gcagcaggcg
     4441 agcattggtg tgcggccgtt gccgatgctg ctgtcgcggc gcctggaagt gagccgcatt
     4501 tccgtggatg gactctcggc gcacctggtg aagcgcggca cgacgaacaa ctggcacgac
     4561 ttgaccgaat ccaagagcac cgccgcaccg ccgccgcgca cggccagcgg ctccgggcac
     4621 gccatctccg tcggcagaat tgaccttcgc cattgcgtcg tcatcctcag cgatgagacg
     4681 gctcacagca cgaccactct gtcgatcgat gagctgcata gcggcgaggt ggcgctgggc
     4741 tcggagaccg gaggactgac gctcgaggac ttcgagctca agggcagcct gctcagggcc
     4801 gccgccggta cgcctgccgc caagccgttg ccgttctcct tgcgcgcacc cgagctggcc
     4861 ctcgaccccc gaacgcaggc cctggcgcct gcgcggatcg aggcgcacgc gggcgagctg
     4921 tcggtcattg tctccgtgtc cgggcagaag acgccggccg gtcaggtgct cagcgggcgc
     4981 atcgaggtcc cgaaagtctc tgcgcgccgg gtgatggatt cgctcggcat cgcggcgccg
     5041 gtgacgcgcg acagccatgt gctgtcggtg cttgcgctca cgagcgggta tcggtttgag
     5101 ccgaaccgtc tggagctctc gggacttgat ctgacgctcg atgacacgca cgtgcaagga
     5161 acggccgcga tcagcgatct ggcgcaggat gcctggactt tcaatctcag cgtcgatgcc
     5221 atcaacctcg atcgataccg cccgcctgcg cccgcggggg gtgcggctgc gcagaaaaac
     5281 ccacccccac ccgggaagca tcccgaagcg cccacaccgc tgcccatcga gactttgagg
     5341 aagctcaacc tgcacggaac actccaggtg ggcagcgcga cggtggcagg cctcatgttt
     5401 accggcatgc gcctgcccgt gtcagcccaa agcggcgcca ttcatctcgg gccagcgcag
     5461 gcattggtct atggcgggac atataacggg gacattgccc tcgatgcgcg cccagcggac
     5521 gcgcaactgt cgttgaatca gcacctgcac ggcaccgaca tcggggcact gttgaaggca
     5581 ggttttgaca caactcggct cagcgggcac gccgatgtga atatggcagt cagcggctcg
     5641 ggcaatacga acgctgcgat catgagctca ctcggaggca agatcgacgc cagcgtactc
     5701 caaggtgccc tcaacggaat cgacatcgct tacgagctgc agagcgcgaa cgctttgctc
     5761 aagcgagaag tgccaccgaa gtcctcgagc ccggcgcgca cccagttcaa catcttgcag
     5821 accagcggca cgctcgagca cggtatcctg cacaccgatg tgctgcgcat cgagacgaac
     5881 tttctcaagg tgagcggcac gggcacgctg aacgtggtga ccgaggcaat cgactaccgg
     5941 ctcgcggccg cactgtccca gggcgcgccg atgagccact ccggtgcagg cccccgcagc
     6001 acagcactgg aggttccgct tctggtcacc ggtaccgctg caaatccgac cgtccggccc
     6061 gacctcgagg cgctcgccaa gggacagctt cgtcagcagg ggggcgatct ggtgcgcaag
     6121 cagctcggcg acaagctcaa gggtctgctg aatcactaag aatcagccac ttagtggggc
     6181 agcaacgccg accggtgcgt gctcgcgcag cgtgagcaca cagcgttgca ggcgtgaacc
     6241 agatcgggac aggcatgcaa tttgctttgt gagtctcgcc tgcatgacag gcgtgctggc
     6301 agcggacgca tgcgaagctg cacgccagcg cgcgtggagc cagcttgagc tagcatcagt
     6361 gaacaattca ccgttgtgac cgtcttaacg taactgaagg gcagggaatt gagggccatc
     6421 acacatcgtg agccagtttc ggatctaagt taacgacgcg cgaactcaac gggagggaag
     6481 gatgatgcaa ccgctctctg aagattcggg ttcccaatcc aaagccaact tgacgttcat
     6541 cgagtctctg agctccttca gccgcctgca ccgcgcgcag cgctgggaag gcacattcgg
     6601 cgatttcctc gccagcatcc tgccggccaa tcccgtagcg atggcacgca ccagtcatca
     6661 gtacatctgg gacatgctgc gctggcacgg acgtgcctca cagcccgatc tcccggacgg
     6721 cgccaatgcg cgcgagctgt tcaagcgcga gctattcgga atcgacgagc cgctgtcgcg
     6781 ggtcgtggac tacttcaagg gcgcggcggc cggctccgac gtgggacgcc gcctgctgct
     6841 gctgctcggc ccgccctccg ggggcaagtc gaccctggcc atcatgctga agcgcgggct
     6901 cgaggaatac agtcgcaccg acgagggcgc catctatgcc atcaagagct cgccactgcg
     6961 cgagaacccg ctgaatctcg ttccggccag cctgcgcccc gagttccgtg agcactatgg
     7021 cgtcgagatc cagggcgagc tctcgccgtg ggcgcgcgag gtggtcgagc gcgaatacga
     7081 cggtgatttc gtgcgcgttc ccgttgagcg cgtatttctg gccgaatctt cacgtactgg
     7141 aatcggcact tacgcgcctc acgatcccac gacggcggac atcgccgacc tggtaggttc
     7201 agtggatctc gccaaggtgg cgcagatcgg cgatgagggc gatccgcgcg cctggtcgtg
     7261 gtcgggggcg gtgtacgctg ccagccgcgg catgctcgag atgatcgaga tcctgaaggt
     7321 caagcgcgag ttcctctatc tgctgctcac gatgacccag gagaagaacg tcaaggtgtc
     7381 gcgcttcccg ctgatacacc tcgatgagac gatcctggcg cataccaacc ttgcggagtt
     7441 ccacaagttc ctgcaggaga aggagaacga ggccctgctc gaccgcatgg tcatcatcaa
     7501 ggtgccttac gcgctctcct accgtgagga gtcgcgcatc taccagaagc tcgtctcggg
     7561 cgcgcccgca ttccgcaatg tccacctcga tccgcacgtt ctcaatctcg cctccgtgtt
     7621 cgccatcctg acgcggctca ccaagaccga gcgcgaaggg ctcgatctga ccaagaagtt
     7681 gcgcctttac gcgggcgagg ctgtggaagg tgtaccggaa tccgaggcct cgcggttgca
     7741 cgcggaatcg gccgacgaag ggctgaccgg cgtcagcccg cggtttgtca taaatgcgct
     7801 ctcgaacgcc atcacacgcg gcaacacgca cagtctgacg agcatggagc tgctgctggc
     7861 gatcaaggat gccatcgaga gcgatgcgcg catcgatgcc aagcagaaga aggcctgggt
     7921 cgatcacctg gtcaccgcgc gcaaggaatt ttacaatcgt tgggtcaagg aggacgtcca
     7981 tcgcgccatg tttgcctcgt tcgaggagga ggcgcagcag ctgctcgaga aatatctcga
     8041 tgaggtcgag gcatcgctcg accaccgcca ggtcaccgat ccgatcacca gcgagagccg
     8101 gccggcggac gagcgctttc tgcgctccgt ggaagagaag atcaaggtat cggactccgg
     8161 caagatgtcg ttccgacagg aagtggtgcg caaggccatg gtcgccttca aggccggcga
     8221 gaagttcaag ctcgcgagcc atgcgcggat gcgcgaggcg attgaacagt atctgttcga
     8281 ggagcgccgc gatgtgctga ggctcgtgac ttccacggca cgcccggatg aggaagcacg
     8341 tcagaagatc tccgtcgtgc agcagcgact cgtgagcgag tacggctacg acgagcacag
     8401 cgcgaaggaa gcgctaagct atgtcacgac gctcctggct caggagtagc gcagcggcag
     8461 cggcgcgagc gtccgacagc gcgcaagatc ctttctggcg tccgtcacga gttgcgcgcg
     8521 gcgcctgaaa ggagtacgaa ttgtccatgg ccgagagcag cgataagccc ggttcgcccg
     8581 atgcggcaaa gtggtatgag ctgttctcgc gcggggcacg cgactggttg cggcacgatg
     8641 agaaagtgcg tgagtcagta cgcaagcatc tgccgcaaat cgtttccgga gcagatgtca
     8701 tcaacgaagg cgcacgcacc gtgcgcgtac cagtacggat gctggagcat taccacttcc
     8761 gtctgcgtcg tccggaggag cagcaaggcg ccggacaagg caaggccaag cccggggacg
     8821 tgttctccga cccctcgcag cagcgcggcc ctggacagaa gggcagtggc ggccaggacg
     8881 agggtgaagt ccaactgctg ctcgagttca agatcgatga catcgtcgac tggctctggg
     8941 aggagatgca gcttcccaat ttgaaggcgc gtgtcggacc gtccgaggag agcgactgga
     9001 tccgcgaagg ctgggatcga cgcggcgctc gctcgagact cgatcggcgc cgttcattca
     9061 aggagatggt gaaacggcgc ggcgccccgg gagccacccc gagcttcatc gacgaagatc
     9121 ttcgttaccg acagcttgcg cgtcgccggc agccggcggt gcacgccgtg gtgttcttca
     9181 tgctggatgt gtcgggcagc atgtccgacc gcgaccggaa gctcgccaag acattcttct
     9241 tctgggtggt gcagggcctg cgccgtgagt atcgctcgct cgagacggtg ttcgtggcac
     9301 acaccacgga agcctgggaa ttcaacgagt ccgagttctt ccaggtgact ggaacgggtg
     9361 gaacggtctc ctccaccgga ttcaacaaag tgcgcgagat catggatgcc cgctacaacc
     9421 ccgggcgctg caatatctac ctgttctacg cttccgacgg cgacaactcg gtcagtgaca
     9481 gcaccctcgc gcgcgagagt ctttccgcga tcaccgggga tgcctgcttc actggctatg
     9541 tcgaggtgtc ctccggtttg tcgcgtcagc ttgcgacgga gacgggtcgg ctgtttgcgg
     9601 agctgcagac ggccggttgc gccgcaggca gctacgcgct gaacgatttc gacgacgtct
     9661 ggggtgccgt gcggcatttc ttcactgccg aaagccgggc gctggaggcc cccggatgac
     9721 cgaggactgg cacgactaca cacagcgcat cgaggagctt gcggtacgct cggggctgag
     9781 cttccatccg gtggatttcg aggccgttcc cgattccttc atgatggaga tcgccgttta
     9841 tggcctgccg gtgcgcatgc cgcactggtc cttcggcgtg cgctacatct accagctcat
     9901 tcagcgccac atgggtcact cacggctgtt cgaagtcgtg tttccaggca accccgggca
     9961 tgcctacctc gctgcgagca acggcctcgc ggagaacatc ctggtcaccg cccatgtgct
    10021 cggtcatgcc gatttctcgc gcaacaatct gctcttccgc cgttgccagg gacaggtgag
    10081 cgagcacatc gtggagcatg ctgccacgca tgcgcgccag atccagcagg caatcgagca
    10141 gtacggcgcg cagcgcgtcg aagtcgttct cgatgccgct ctcgcgctgg agcagcatat
    10201 cgatgttgac cagtcgttac gccgcgagcg ttatccggag tacgaggcgg aaaacaaggc
    10261 catcgtcgat gatgacttcc gcaaacgctt cgccgcgctc gatccggcag ctgccgccgg
    10321 agcgctcgag attcgcaaac gcgcgcccgt gccgccgcat ccggagcgtg acctgctgtg
    10381 gttcgtggcg aactatgctc cggagatgga gagctgggag cgggacatct ttctcgcggt
    10441 gcgcgaggag tcgttctact tttatcccgt cttcgccacg cagatcatga atgaaggctg
    10501 ggcctcgtac tggcacgcga gattactgcg tgaagcggag ttcgtcccgc agcaggcgta
    10561 tgtcgatgcc atcaaatgcc actccgacgt cgtccgtccc gtcgccgccg atcagcaggt
    10621 ggcgctcacg atcaatccct accatctcgg ctttgcgatg tgggagaaga tcgtcgagaa
    10681 ggaaggcctg gagggtgcgc gcggcatcat gcaacaggac gatgatttcg gcttcatccg
    10741 taatcacctg acgcgcgagc tggcggacga gctgggcctg ttccgcttca acgcccgcag
    10801 cgacgggcag gtcaaggtcc tcgaggccga catgtctgcg ctgcaggagg cagtgctcgg
    10861 ccccaaatac aacttcggcg ccccgagcgt atccgcggcg catgtgcgca atgacggcac
    10921 cctggagctg atgcacgaca gcgcgagcga tgggcgaggg ctcgatctcg aacgcagccg
    10981 acgagtgatc gactacgtcc agcgtgtgtg gcgccgcccg atcacactca ccaccgtcaa
    11041 tcaggctggc tcgactctcg agttgacggc gccgtaattt cattgcagaa acacgcctac
    11101 ggcgctttct gcccgcgact gacgcgcatc ccgatcccgg gcggcgcgac aaaagccgtg
    11161 gcttttgtcg ctcaatcccc cacgttgaat gcattagcgc ggaaatggcc tgcggccatt
    11221 tccgcaggcc agacatcgaa gttgcttcag gccgacctct atttcttcgg ggtctcttcg
    11281 gcctcgtccg gatcggacgt ccagtcttcc tcattttgtg aggtgacgat cgagacgggg
    11341 tcgctggccg gaaaggattc tgcgagcgcc tcgtcgagct gctgctgcac tttcttgcgg
    11401 tgacgctcgc gggcctcggg ggtgggttcg gtcatcgggc gctcctctcg ctgggccttc
    11461 cccgaagact acttgatgtg cgggcctgcc ggatagtttc cggcaggccc gctgctcgac
    11521 cgtcaaaagt cggtaggacc gcttttgacc ctcccctggc cgtcaaaagt cggctgcgcc
    11581 gcttttgacc gggtcccgaa agggctgact ttcgtgaccc tttccccctc gcttcgaacg
    11641 gctagaactt gtacttcgcc cgtacgttcc agaacgcacc gggcagatca taggtgccgg
    11701 catcgtaacc gttcagtgtg caggtgaagc acaccggcgg attgacgccg aacagattgt
    11761 tgacacccgc ttccaaggtc aggtccttca accagaaggc gttattccag gcgagctgcg
    11821 cgtcgttgta ggtgaccgca tgcagagtat gaacgtcggt ggtggtctcg caaccgggca
    11881 cgccgacttc agccgcatta ctgcaggctt cggtgaccgc cgacaggtag cgcaccgtcc
    11941 aggtgaactc tacgtccgcg atgccgtagc cgagctgagc attcatgcga taacgcggaa
    12001 tcgcgctgtt gctgacttcg atgcccacct gccgctgcgc caccagaccc agcgtgtcga
    12061 ccgccttgta gctgttgact cgtgtggcct gcacggcggc cgagaagtga ccgatgggca
    12121 gtggctcggt gagccagttg agcttcagat cctcgccatc ggtggtgatg tggcccaggt
    12181 tggccaggaa gttcttcggc ggacggagct ggccgcccgc gccgcgcgtg aacggcgcgc
    12241 acagcaacgg atccgtaccg ccggcatcca gacagctgtt gaggagcgcc tggatgtctt
    12301 ccgcctcgat ggctcccttg atcttatggt tgtagtaagt cgtttccagc gtcaggcggg
    12361 cggtgtgcat catgccttcg gcccagcctg cgttgtaaac gaatccggca gtgaagctgt
    12421 cggatttctc cggacgcaga tccgcatttc ctcccgtgaa ggtcgtgatc tgcgtattgg
    12481 cctgctggaa gttattcggc acgccctgcg cacggcaggc gttcgcgagc gcggagtggt
    12541 tcccgggcgt gacgatgatg gtgccggtcg ggccacaggg gtcgaccagc gtcgcgccga
    12601 attgcgtcag accgaacagc tcaccgagat tcggggcgcg gaagccagtt gagtacgtgc
    12661 ctcgcaggcc gaagtcctgg atgggctgcc agcgcaaacc gcccttgtag gtagtggtgc
    12721 tgccgaaatt cgagtagtcg gaatagcgga tcgccgcgct ggcgcccagg gtctccagca
    12781 gcggggcgct cacttccccg tatgcctcgt tcacacgaaa actggcgtcc accggaaatg
    12841 ccagcgagtc ctgcgacaga ccctccgtgc gcagcggatc cggggagaag tctcccttgt
    12901 agaggcgatg ctcggcaccg atcgcaatgc ccaccggacg atcctggatg ctgaaaggat
    12961 gacccgtgat gttggcggag aagatgcgca gatcctgctc actgcgatcg atctgcaccg
    13021 cttcgatgaa cttcagcatc gtcggggtga tcgggcggcc ctggccgccg aacagatcca
    13081 gcggcacgca gttgggcacc tgcgcgcaca ctgccggatc gcccagcgcg atcgcgatgt
    13141 tgccgatgtt gtacccaccg gtgaacgtct ggttcgcctt gttggcggag ttgacgtagt
    13201 tcacgtccca gcggtactcc gatcccaaca tgttgaagtt gccgcggaga cccgtgccga
    13261 agtagtaggt atcgacgttc tggtcgaaga tgcgcggccc ggcttccagc ggccggcgcg
    13321 tgacccaacc gaaattgctg gccgcatcca ggtcgatgcc gaacgggtta taggggttct
    13381 tggcggagat attgatcgtg tcggcgaggc cgccggtgcc ggcgtacgga ccgacgaaga
    13441 tcggctcggg cgccgcctgg ttctcggact tgcgattgtt gtacagcccc ttgacgtaga
    13501 cctccacgtt gtcactggcc tgatagctca ggttggtgaa gatcgacttg cgcgtctgcg
    13561 gcgtgagcag caggttgaag ggcgcgaagt tgaagcgatc cgcgttgctc caggcgtgat
    13621 aggtgctgcc ggctccgaac gggttgaggg gattccagac aggggtcgtc gtgccgttgt
    13681 tgagcgtcat gtcgtagaag ttgccctgat ccggggtgca cgagcccaga ttcggcaccg
    13741 cgacccgtgg gtcacagaac gtgtagcgtc cctgcggggt cgccgagctg ccggcgcgca
    13801 ggccggcgaa aggctccggc acatccgatt gccaccattt cgaggaactg atggcatcct
    13861 ggttgtagta gctggcgacg aacatacccg agaacttatc gctcgagccg ccgacgctca
    13921 ggctgccctg tctggtctgg ccgcctttgc cgtattcgcc cgtgtagccg ttcagctcca
    13981 ctccatccat tttcttcttg gtgatgatgt tcaccacgcc cgcgatcgcg tccgagccat
    14041 agatggacga ggcaccgtcc tccagcacct cgatatggtc gatgatcggc agcggaatgg
    14101 tgttcaggtc cgcgctgccg ctgacaccgg aggcggacga ctcgttcacc cagcggatgc
    14161 catcgaccag cacgagcgtg cgcttggaat cgagattgcg gagatccacc tgagtggagc
    14221 ctgcgccgat accgccgccg tccggcgggt aaccgaagtt gccggatgag ttgaatttgg
    14281 cgttcagcgc ggagccgccg gtcgtgagct gctgcagcaa gttgccgacg tcggccaggc
    14341 ctgttcgctc gatcgcatca ttgctgatga ccgacagcgg ctgctcccgc tgcgcttccg
    14401 attgcgcaat acgtgagccg gtgacgatga cttcctggat tttgtcgtcc gccgccgcag
    14461 gcgccgcctg cgagagggcc acttgcggag cgactcccgc tagtagcacg cacgcaacga
    14521 acgagattcc accattcccg atctggatct tcatgaagca ctccccgagg tgttcgccat
    14581 ccagggtcgg ggactcgctg cgttcccgcc cggagttatc cgcggactct ataggcgata
    14641 gcaaggggaa gtaaagaaat atgacgaatc ccaagccggt gggacgaacc tgaggtcagc
    14701 gaggatggcg tggggaaagt tgcggcaggc gacccagggt ccgcagtgtt gcagacctgc
    14761 cacagtgggg cgctggacgg tggcgcgcag acccgtaaat agcgctcaga agcgagccga
    14821 gcgcgagcgc ccgagcccag gcccgcaatg gctcccgcgc cgcagcggga gtaggctttc
    14881 gaccaagtga ctggggtccg gagcagccgc catggggtat accacgcagg acatccgaaa
    14941 cgttgcgctg gtcggtcacg ccggcggcgg ca
//
LOCUS       DASRHN010000208         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1392759, whole genome shotgun sequence.
ACCESSION   DASRHN010000208 DASRHN010000000
VERSION     DASRHN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392759"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFB37_08000"
     CDS             complement(<1..962)
                     /locus_tag="VFB37_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22436.1"
                     /translation="MLPETESCQTPTPYESSPVLLRLYVFLVMLLLPMDWFAPTGMLF
                     REFGAKPATLLLTLGGICGMAFVRKGRRLAGVSVELIALVIFSAWLALGFLSALANFI
                     GGWSDWHSDRSPFGQLINQSALVVVCGIAVIGNARIFCSYPAVSRLVARYLPLAMLLH
                     LFAFGLNATGVVSQSSAVFAPFRAVEAHDTNRPTGLFSEPAYFGTFAALYGAALWSLP
                     VGRLRKSFYVLLALGAFGASIAIGAKTFVVVLGAQAMYYVLHRTRSLISGIASAFILL
                     LIVAVGIYFIQVYSALDVRSNLSSADRLGSAMLATNVAAQGYALP"
     gene            complement(973..1692)
                     /locus_tag="VFB37_08005"
     CDS             complement(973..1692)
                     /locus_tag="VFB37_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZO22437.1"
                     /translation="MLRQFLRSTLRHVGLDVRRYRPATSADAALKAMLAFHRINLVFD
                     VGANTGQFGALLREAGYAGRLVSFEPLSAAHLQLTKVARHDPLWEVASPMAIGSENGE
                     IELHVSGNSVSSSVLPMLEAHSSAAPESAYVSSEKVPLRTLDTVAAAYLRPDTELFIK
                     IDTQGYEDRVLRGAGTVLQRTKGLQLELSLVPLYEGQRQFDDLLAELQHAGFELWNMT
                     PAFVDPVRGRLLQVDATLFRR"
     gene            complement(1703..2641)
                     /locus_tag="VFB37_08010"
     CDS             complement(1703..2641)
                     /locus_tag="VFB37_08010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO22438.1"
                     /translation="MNQLADLVLLPFVLPAGLVLKAVRRIGLRRLRWCSKLLLRIGVL
                     PIRRHYYEPFIDPGELRRPLDEERRLPGIDWNLSAQLQLLETLRYEGELTYLTAPRTS
                     AIDFRLNNGSFESGDAEFLYQLIRARKPRRLFEIGSGNSTLIARDAIGRNAQESAGYS
                     CKHVCVEPFEAPWLESAGVTVVRKRLEEVDPAMFAELEENDILFIDSSHVIRPQGDVL
                     VEYLEILPALRPGVIVHIHDIFSPRDYPSEWVKDRILFWNEQYLLEGFLTQNDSWTIL
                     AAVNLLRHRHFEALRRVCPYLTPEREPGSFYMQKVR"
BASE COUNT          595 a          894 c          879 g          444 t
ORIGIN      
        1 ggcagcgcat agccctgcgc ggcgacattc gtggcgagca tggccgagcc caaccggtcg
       61 gcggagctca gattcgaccg cacatcgagc gcggagtaga cctggatgaa gtagataccg
      121 acagccacga tgagcagcag aatgaaagcg ctcgcgatgc cactgatgag tgagcgcgtc
      181 cggtgcaata cgtagtacat cgcctgagcc cccagaacaa ccacgaacgt cttggcgccg
      241 atggcgatcg aggcgccgaa tgcgcccagc gccagcagta cgtagaagct ctttctgagg
      301 cgccccaccg gaaggctcca caacgccgct ccatagaggg ccgcgaacgt tccaaaatag
      361 gccggctccg agaacagacc cgtcggcctg ttggtgtcat gagcctccac ggcccggaat
      421 ggcgcaaaca ccgcggagga ctgcgacacc acgcccgtgg cgttgagccc gaaggcgaac
      481 agatgcagca gcatggcgag cggcagatag cgggcaacca gccgggagac tgccgggtag
      541 ctgcagaaga tgcgggcatt gccgatgacg gcgatcccgc ataccaccac cagggcggac
      601 tggttgatga gctggccgaa cggactgcgg tcggagtgcc aatcggacca gccgccgatg
      661 aagttcgcca gtgccgagag gaaacccaac gcgagccagg ccgaaaaaat cactaacgcg
      721 atgagctcga cagagactcc cgcgaggcgc cggcctttgc gcacgaatgc catgccacag
      781 attcccccca gggtcagcag gagagtggcc ggttttgcgc cgaactccct gaagagcatg
      841 cctgtggggg caaaccaatc catgggcaga agcagcatga cgaggaagac gtagagccgc
      901 agcagcaccg ggctgctctc gtagggtgtc ggtgtctgac aggactcggt ttcgggcaac
      961 atcggccggc cctcagcgcc gaaacagggt tgcatcgacc tggagcagtc ggccacggac
     1021 cggatcaacg aaggccggcg tcatgttcca gagctcaaag cccgcgtgtt gcagctcggc
     1081 gagcaggtca tcgaactgcc gctgtccctc gtagagaggc accagcgaca gctccagctg
     1141 cagacctttc gttcgctgca acactgtccc ggcgccccgc agcacgcggt cttcataacc
     1201 ctgtgtgtcg attttgatga acagctcggt atccggccgc aggtacgcgg cggcaaccgt
     1261 atccagagtg cgcagcggca ccttctcgct gctcacgtag gccgattccg gcgccgcgga
     1321 ggaatgggcc tcgagcatgg gtagcacgga gctgctgaca gagttaccgg agacgtgcag
     1381 ctcgatctcg ccattctcac tgccgatcgc catgggcgag gcgacttccc agagcggatc
     1441 gtgccgggcc actttcgtga gctgcagatg cgcagccgac aaaggctcga aggagacgag
     1501 ccgtcccgca tacccggcct cgcgcagcaa ggctccaaac tgcccggtat tcgcacccac
     1561 atcgaacacc aggttgatgc gatggaacgc aagcatggcc ttcagggctg catccgccga
     1621 ggtggcgggc cggtaccggc gcacgtccag tccgacatgc cgcagcgtcg atctgaggaa
     1681 ctgtctgagc attccagcgc gactacctta ctttctgcat gtagaaggag ccgggctcgc
     1741 gctcgggtgt cagataggga catacccggc gcaaagcctc gaagtggcga tgtctgagca
     1801 gattcacggc ggcgaggatc gtccacgaat cattctgtgt caggaaccct tccagcaggt
     1861 actgctcgtt ccagaacagg atccggtcct tgacccattc actgggatag tccctggggg
     1921 agaagatatc gtggatgtgc acaatgactc ccggcctgag agccggcagg atttccagat
     1981 actcgacgag cacatcgccc tgcgggcgga tgacatggga ggaatctatg aagaggatgt
     2041 cgttctcctc cagctcggcg aacatggccg gatccacctc ctccaggcgc ttgcgcacca
     2101 ccgtgacgcc ggcgctctcc agccagggtg cctcgaaagg ctcgacacag acgtgcttgc
     2161 acgagtagcc cgccgactcc tgggcatttc tgccgatggc atcccgcgca atcagtgtgg
     2221 aattgccgct gccgatctcg aacaggcgac gcggcttgcg ggcacggatc agctgataga
     2281 gaaattccgc gtcccccgac tcgaacgagc cgttgttcag gcggaagtcg atggcgctgg
     2341 tgcggggagc cgtaagatac gtcagctccc cctcgtagcg cagtgtttcc aacagctgca
     2401 gttgcgcgga caggttccag tcgattcccg ggaggcggcg ctcctcatcg aggggtcggc
     2461 gcagctcgcc cggatcgatg aacggctcgt agtagtggcg cctgatcggc aggacgccga
     2521 tccgcagcag cagcttcgaa caccagcgca ggcgccgcag ccctatccgc cgcaccgcct
     2581 tcaacaccag gcccgcgggc aacacgaagg gcagcagcac gaggtccgcc aattggttca
     2641 tgaccgctgt cgcaactctc gccataccca tacccggtgt cacatttacc gcagcaccat
     2701 cgagcgaacc tggaggaaga acacgggtcg cgatgctgcg ctcgccgcgt tcatgatgaa
     2761 accggtcagg caatagctca ccagtgttgc aacagccgca ccgaccaccc cg
//
LOCUS       DASRHN010000209         5668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1392778, whole genome shotgun sequence.
ACCESSION   DASRHN010000209 DASRHN010000000
VERSION     DASRHN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392778"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFB37_08015"
     CDS             <1..195
                     /locus_tag="VFB37_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22439.1"
                     /translation="LTPNLLGGKNSQWFTELIYTQFIVRFNWEQGAAFGFLLLGLSTT
                     MVAIGLRLSGQRFVDVMRRS"
     gene            215..1096
                     /locus_tag="VFB37_08020"
     CDS             215..1096
                     /locus_tag="VFB37_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO22440.1"
                     /translation="MIASLHRSAGLRAATTLYVVAFLIFLFLPLLTVAAFAFNDAPYP
                     APPWRGFTLDWFFGDAAKARVGLFHDTELLGSIWTSVVVACWVTALSVCIGTANAFLM
                     ERCQFAGKQALSVLMLTPLVIPGVILGISILSLSSRVAELADDAFGLDLDFLRPGLPL
                     VVLGQFSYIATIATLMISARLKRFDRTLEEAALNLGASHTAVLRTITFPYLRPALIGS
                     AAIAFLMSFENFNTTLMLVGSDSPLTIMMYGRMREGASPVLNAVSLFLMLASAALALT
                     LLRGSSRAAPAEARGKS"
     gene            1108..2295
                     /locus_tag="VFB37_08025"
     CDS             1108..2295
                     /locus_tag="VFB37_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZO22441.1"
                     /translation="MRLTGIRTFVVGNPPPHYGGRYFVFARLTTDAGVEGVGEAYCVP
                     FEPHLVAKMLEDVFERYLCEEDPHDIERIWRRVYSAGFTQHPDLTLMGVLSALEMACW
                     DIIGKEAGQPVYRLLGGRVHERLRTYTYIYARPGDRADVYSDAELAAERAAEYLQQGF
                     TALKFDPAGPYSAFDGRQLRLPDLDRCERFARLLREAVGSRADLLLGTHGQMTAAGAI
                     RLARRLEPYDPLWLEEPVPPDAPHEMARVARGTTIAIAAGERLATKYDFARLLQAGAA
                     AILQMNLGRVGGLLEGKKIAGMAEVYHVQIAPHLYCGPIVGAANIHLATCSPNFLILE
                     GIERWDGFHAQILRKPIRWENGYVIPPSEPGLGVELNDEVIVKHPYTGKMLHLEMADR
                     PVC"
     gene            complement(2329..3948)
                     /locus_tag="VFB37_08030"
     CDS             complement(2329..3948)
                     /locus_tag="VFB37_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZO22442.1"
                     /translation="MSYDYIIVGAGSAGCILAARLSESGRHSVLLLEAGGRDDALRFR
                     IPIGYVYTYYDPNANWMYYLQPEPALGNRSVYCPRGKVQGGSGSINALIYIRGQRSDY
                     DDWVAGGNPGWAYQDVLPYYRKLESHPLGDTPFHAADGPIHITRMLADAHPICAAFLQ
                     GCEGLGYSRNEDFNGASLEGVGIYDINTHRGLRDSSSRAYLHPALRKERLRFEPRALV
                     EKILFEESGRAKGVRVQQHGAVHEFMAAREVILSAGAIDTPKLLQLSGIGDQALLSKH
                     GVRVVHHLPAVGRNLQDHICASYYYKANRRTLNDELGSWIGRARAALQFALRRRGPLA
                     MSVNQAGGFLRSSAERPTPNIQLYFNPLSYQIPKGRGSTLRPEPYSGFLLAFSPCRPS
                     SRGSVEIASGAVQDSPLIRPNFLSTQQDIDEVIEGSRLVRKLMQAPALRAITVEETKP
                     GPHVTDDAQMLQYFREEAGSIYHPCGTCAMGSDPRTSVVSGRLKAHGIDGLRVVDASV
                     FPNITSGNINAPTMMVAERAAEMILQDARNG"
     gene            complement(4036..5400)
                     /locus_tag="VFB37_08035"
     CDS             complement(4036..5400)
                     /locus_tag="VFB37_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO22443.1"
                     /translation="MKSISEPGRSVPVVLETDVLVVGSGPGGLAAAIAAARAGVHTAL
                     LDRNGCFGGNITQVGVEGFAWYRHEATVDSEGIGIEFEQRAKAMGAALPEPQSVSYAL
                     DAEMFKYVADVLVQEAGITPLLHRLCVAPIIEDGAIRGVITESKSGREAILARRIVDA
                     SGDADVAAWSGAPYRKTAKEEMMAASVMFSMAGVNKRRFLEQIKADPQTYRDWAGNAE
                     WDIETTGKEDHLFSPFLRKPFKQAVAAGVIPSTLSTIAGTWGSISEQGDLTYLNLIHL
                     PCIDGTDASDLTAGEIEGRAQAIHAVEALRRFMPGCEEAKLRNFGMTIGIRDTRKIDA
                     LYNMTGQDVREQARFEDSIGIFPEFIDGYGILILPTTGRYFHVPYRALVPQGIANLLV
                     AGRCIGGDKVSHAAVRNMACCAVSGQGAGTAAAISVKTDRPFDRLDIGVVQRELARQG
                     ARIQ"
BASE COUNT          980 a         1812 c         1783 g         1093 t
ORIGIN      
        1 ctgacaccca acctgctggg aggcaagaac tcccaatggt tcaccgagct gatctacacg
       61 cagttcatcg tgcggttcaa ctgggaacag ggggcggcgt tcggctttct gctgctcggg
      121 ctgtccacca cgatggtcgc aatcggcttg cgccttagcg gccagcgctt cgtcgatgtc
      181 atgaggcgct cctgatgagc cagggagcgc gcccgtgatc gcatcgctgc atcgctcggc
      241 cggattgcgg gcagccacga ctctgtacgt ggtggcgttt ctgatctttc tgtttctgcc
      301 gctgctcacc gtggccgcct tcgctttcaa cgatgcaccc tatcctgcac cgccctggcg
      361 aggcttcacg ctggactggt tcttcggcga tgccgccaaa gctcgtgtcg gcctctttca
      421 cgataccgag ctgctcggca gcatctggac cagtgtcgtg gtggcgtgct gggtcacggc
      481 tttgtcggtg tgcatcggca ccgccaatgc cttcctgatg gagcgctgtc agttcgcagg
      541 caaacaggcg ctctcagtgc tcatgctgac accgctggtc atccccggag tcattctggg
      601 catttccatc ctgtcgctct cgagccgggt agccgaactt gctgacgatg ccttcgggct
      661 cgacctcgac tttctgcgtc ccggcctgcc actggtggtg ctcgggcagt tctcctacat
      721 cgctaccatt gccacgctga tgatttcagc gcgtctgaag cgctttgatc ggacactgga
      781 agaggcggcg ctgaatctcg gtgcctcaca tacggcggtg ctgcgcacga tcacctttcc
      841 ctaccttcga ccggccctga tcggctcggc cgcgatcgcg ttcctcatgt cgttcgagaa
      901 tttcaatacc acgctcatgc tggtcggatc cgattcgccg ctcaccatca tgatgtacgg
      961 ccgcatgcgc gaaggggcca gccccgttct gaacgctgtc agtctgtttc tcatgctggc
     1021 ctccgccgcg ctcgccttga cgctgctgcg tggaagttcg cgcgcggcgc ccgccgaggc
     1081 gcgcggcaag tcctgacggg ccaacggatg agactcaccg gaattcgcac gttcgtcgtc
     1141 ggcaatccac cgccgcatta cgggggtcgc tatttcgtgt ttgccaggct gacgacggat
     1201 gcgggggtcg agggtgtcgg cgaagcctat tgcgtgccat tcgagccgca cctggttgcg
     1261 aagatgctcg aggatgtctt tgagagatac ctctgcgaag aggatcctca cgatatcgag
     1321 agaatctggc gacgcgtcta ctctgcagga ttcacacagc atccggacct gactctcatg
     1381 ggcgtgctga gtgcgctcga gatggcctgc tgggacatca tcggcaagga ggcaggacaa
     1441 ccggtatacc gcttgctcgg cggccgggta cacgagcggc ttcgtaccta cacgtacatc
     1501 tatgcgcggc ccggggatcg ggcggatgtc tattcggatg cggagcttgc ggccgagcgt
     1561 gctgcggagt acctgcagca gggctttacg gctctcaagt tcgatccggc cggaccttac
     1621 agcgcctttg acggacgcca gctccggctg ccggatcttg atcgctgcga gcgatttgca
     1681 cgactgttgc gcgaagcggt cggcagccgg gcagacctac tgctgggtac gcatggccag
     1741 atgaccgcgg cgggggcgat ccgactcgcc aggcgcctcg agccgtatga cccgttgtgg
     1801 ctcgaggagc cggtgccacc cgatgcgccg cacgaaatgg cgagagtcgc gcggggcacc
     1861 acgattgcga tcgccgcagg cgagcgcctc gccaccaagt atgactttgc ccggctgctg
     1921 caggcaggcg cggcggccat tctgcagatg aacctcggtc gtgtcggcgg ccttctggaa
     1981 ggcaagaaga tcgccggtat ggccgaggtc taccacgtgc agatcgcgcc acacctgtat
     2041 tgcggaccca tcgtcggcgc cgccaatatc catctcgcca cctgcagtcc taacttcctg
     2101 atactcgaag gcatcgagcg ctgggacgga tttcacgccc agatcctgcg caagcccatc
     2161 cgctgggaga acggctacgt cattcccccc tccgagccgg gcctcggcgt ggagctgaat
     2221 gacgaggtca tcgtgaagca tccctacacg ggcaagatgc tgcacctgga gatggcggac
     2281 cgaccggtct gctgatcgtc gggtactttc ccactcgact tgaggcgctc agccgttgcg
     2341 ggcatcctgc aggatcatct cggctgcgcg ctcggcaacc atcatcgtcg gcgcgttgat
     2401 gttgcccgag gtgatattgg ggaatacgga tgcatccacg actcgcaggc cgtcgatgcc
     2461 atgggccttc agccggcccg agacgacgct agtgcgcgga tcggatccca tcgcgcaggt
     2521 cccgcagggg tggtatatcg agcccgcctc ctcgcggaag tactgcagca tctgcgcatc
     2581 gtccgtcacg tgcggaccgg gcttggtttc ctcgacggtg atagcgcgca gggcgggcgc
     2641 ctgcatcaac ttgcgcacca gcctgctgcc ctcgatcacc tcatcaatat cctgctgtgt
     2701 ggacagaaaa ttgggacgaa tcaacggcga atcctgtacc gcaccggaag cgatctcgac
     2761 gcttccgcgg ctgctcgggc ggcaaggcga gaaggccagt agaaagcccg agtaaggctc
     2821 gggccgaagt gtggagccgc gacccttggg gatctgatac gagagcgggt tgaagtacag
     2881 ctgaatgttc ggcgtaggcc gctcggcgct gctgcgcaga aagccaccgg cctggttcac
     2941 actcatggcg aggggtccac gccggcgcag cgcgaactgc agggctgccc tggcacgccc
     3001 gatccacgag ccgagttcgt cattcagggt cctgcgattg gccttgtagt agtaactcgc
     3061 gcagatatga tcctgcagat tgcgaccgac cgccggcagg tggtgcacca cgcgaacgcc
     3121 atgtttggag agcagagcct ggtcgcctat tcccgagagc tgcaataatt tgggggtgtc
     3181 gatcgcccct gcgctgagaa tcacttcccg cgctgccatg aattcgtgca cggccccgtg
     3241 ctgctgaacg cgcacgccct tggcccgccc ggactcctcg aacaggattt tctcgaccag
     3301 cgcacgaggc tcgaacctca agcgctcctt gcgcagtgcc ggatgcaggt aggcgcggct
     3361 gctcgaatcg cgcagtcccc gatgtgtgtt gatgtcatag atccccactc cttcgagcga
     3421 ggcgccgttg aaatcctcat tgcgtgaata gccgaggcct tcgcaccctt gcaggaaagc
     3481 ggcgcagatc gggtgggcat ccgcgagcat ccgggtgatg tgaatcggcc cgtcggcagc
     3541 gtggaacggc gtatcgccaa gcggatgcga ctcgagcttg cggtaatacg ggagcacgtc
     3601 ctggtacgcc caaccgggat tgccgccagc cacccagtca tcgtaatcgc tgcgctggcc
     3661 gcgaatgtag atcagtgcat tgatcgagcc actgccgcct tgcaccttgc cccgcggaca
     3721 gtagaccgag cggttcccaa gcgccggctc gggctgcagg tagtacatcc agttcgcgtt
     3781 ggggtcgtag tacgtgtaga cgtacccgat cggaatccgg aagcgcaacg cgtcgtcgcg
     3841 accccccgcc tccagcagca gcacggaatg gcgacctgat tcagagagtc gggcagctag
     3901 aatgcagccg gccgagccgg cgcccacgat gatgtaatcg tagctcacga tcgcctgtcg
     3961 ctcgccggcg ccaggttcac gagcctaccg gcagtggact gaatggctgc agcccgattc
     4021 cgatcctgcg tgccctcact gaatacgtgc accctggcgg gcgagctcgc gctgcaccac
     4081 gccgatgtcg agtcgatcga aaggccggtc ggtcttcacc gatatggcag cagcggttcc
     4141 ggctccctgt ccgctcaccg cgcagcaggc catgttccgc acggcggcgt gcgagacctt
     4201 gtctcctccg atgcagcggc cggcgaccag aaggttggcg atgccttgcg gcacgagcgc
     4261 ccggtaaggc acgtggaaat agcggcccgt ggtcggcagg atcaggatgc catagccgtc
     4321 gatgaattcc gggaagatgc cgatcgaatc ctcgaagcgc gcctgctcac gcacgtcctg
     4381 cccggtcatg ttgtagagcg catcgatctt gcgcgtatcg cggatgccga tggtcatgcc
     4441 gaagttgcgc agcttcgcct cctcacaccc gggcatgaag cgccgcagcg cttcgacggc
     4501 gtggatggcc tgcgcgcggc cctcgatctc ccctgccgta agatcactgg catcggtgcc
     4561 atcgatgcac ggcaggtgga tcagattgag ataggtcaga tccccctgct cgctgatgga
     4621 gccccacgtg ccggcaatcg tgctgagcgt cgaggggatg acaccggcgg ccacagcctg
     4681 tttgaatggt ttgcgcagga atggcgagaa cagatgatcc tccttgccgg tggtctcgat
     4741 gtcccattcc gcattgcccg cccagtcgcg ataagtctgc ggatcggcct tgatctgttc
     4801 caggaagcgg cgcttgttga ctccggccat ggagaacatg accgaggcgg ccatcatttc
     4861 ctccttcgca gtcttgcgat agggagcacc tgaccaggca gcgacatccg catcgccgct
     4921 cgcatcgacg atccgccgcg caagtatagc ctcgcgtccg gacttgctct cggtgatgac
     4981 accgcggatg gccccatcct cgatgatcgg cgctacgcac aatcggtgca ggagcggcgt
     5041 gatccccgct tcctgcacca gaacgtccgc cacgtacttg aacatttcag catcgagggc
     5101 gtagctgacg gattgcggct ccggaagtgc cgcgcccatg gctttggccc gttgctcgaa
     5161 ctcgatgccg atgccctcgc tgtcgaccgt ggcctcgtgc cgataccatg cgaagccctc
     5221 gacgcctacc tgagtgatat tgccgccgaa gcagccgtta cgatccagca gagcggtgtg
     5281 cacaccggcg cgagcggcag cgattgcggc ggcgagccct cccggcccac tgcccacgac
     5341 gagcacgtcg gtttccagaa cgaccggcac cgaccggccc ggctcggaga tcgatttcat
     5401 ttcgcccgga ctcctttgac gaccgctagc gggcaggtgc ggaaggtcgc agcaggcgat
     5461 cgggaatgat ggcccggagc gcgatcgacg ccctcctccc gcggtaatca ttgggctgga
     5521 tcattgtcgg caggtgacga tcagcgatgt agcccggtcc catgcccggg atcgtcccca
     5581 gcgcccgtga ggtattcaag ccagccgtgg aaagcgcgcc atgcgttcga gatcgttgag
     5641 ctcctggtca ttgcggatgt agaggcgg
//
LOCUS       DASRHN010000210         7805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_235953, whole genome shotgun sequence.
ACCESSION   DASRHN010000210 DASRHN010000000
VERSION     DASRHN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235953"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFB37_08040"
     CDS             <1..705
                     /locus_tag="VFB37_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZO22444.1"
                     /translation="VRFGASGHNSVVSNNEFRDDKDAGLWAVRSAPDSHDDIIGIHDN
                     KFVEDGTGIVAGNIPVLVERNDFVNAHEADVHVVGAGAVVRGNRINGGASMGIVAENA
                     RGAVIDDNELEGLTAYGVMVRGSSNTLVRSNRLHNCGYGLAFVLGDSHGVSTAVDNTI
                     IEPKFNGIDVIGDSPILRRNQVLRPHAFALHVEDFQPPNGQKVQSQPFLDNNNFGNSP
                     VSRSSTATAMAPSMMR"
     gene            718..2127
                     /locus_tag="VFB37_08045"
     CDS             718..2127
                     /locus_tag="VFB37_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZO22445.1"
                     /translation="MRPNVDSTQLIPRPVWENPRVHLLDDIWLLTIVAILVATGVPWF
                     ASGFEVEVGSASWGLLALGGIHVAFTMLASPTHSPGHWRDRALTILDVAGVILIGFIW
                     QHVGALQNPMFLTVFALPVMGAIFLSRWHPYLIATVSLLVVAVVAMIQAPELRWYASG
                     LIGNEDWLTRLFGQQSAAPQSSFSGFYAPSGYLVVLLEVFAVILFACAVAAEYVGTIF
                     ERLNAHIVMARTEAERGQELWADLIERLPLPALLVDPDTLRVVASSNFAIAYLSTGEM
                     PLEGRNLFEALQFSYPDIVQELIVGSDGGAPLTFLRIEDQLRVTQVRVLHVAHKGRRL
                     ALLTIEDATEVFCLRAVLDTSEYAAVVIDAGGRLLAFNKPALALFGGAEVGMDAAELL
                     PQPETGLRWWEPGLTGRRKMHLEIGPRIYQVTSSAIALMGEEERIFTASFLPVAKAGT
                     TDPFGTSSTMVTSTLGQVR"
     gene            2124..2942
                     /locus_tag="VFB37_08050"
     CDS             2124..2942
                     /locus_tag="VFB37_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22446.1"
                     /translation="MRRVKSAMFMLTVGGLLSGGAFASDDRIAVSGDGASLTGTDGGF
                     GGSLGWLHNFDPDTLLGIAGEHQKLGPSQWSFGSLTGAMTNGEGDQRYTVSGEVHEGA
                     GVDTHKHFNYAIEAVGLTGTYYHRLSAQLEDKQINVETTHGNLPKAGLSYLWNPHFQS
                     QISYSYSFGGNLGTRLTAARIDIFGKHVNFLTGGAWGTASPIVLGVQTILPPRQTKEG
                     YVGLAIPVPSLRSEVTLIGDYLFIKGGVISAGVVPDSTKWTGTLNWVFHVGGKK"
     gene            2939..5305
                     /locus_tag="VFB37_08055"
     CDS             2939..5305
                     /locus_tag="VFB37_08055"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZO22447.1"
                     /translation="MTRLTRKSELGWLPTGVVLLVVLLAGSRLIYLSVQHHAAAARQS
                     AAAIAANFVKRIEPRLSNLAYHAAHQAALANKVLSGPNASLQSTPLAPHTFWMTPDDK
                     VLGARRGEMPTASGIASEWQSADSNRPVAGSSLLGPMRLGSVWLLATRVPVVALDESQ
                     QPQTRGWSIAYADLDEIIADSHLARLVDLGYEFSLSQVEPRSARSRIFVGSVTDPLTD
                     PVTTRIPLPSGVSPTIAGSYLELAIQPRSGWYPATELASDIGLLAFLAWLLAFGTHDL
                     SHALERSRAALKGARRRLRAIHHQLATEMQQRLSLQETFDHARFHDAFTGLPNRRYFM
                     DQLDRALREVRTKRRQRIAVIIVDIARFKLINDMLGHTAGDELMVQAARRFEKAAKTF
                     EGVLARWGGDQFALLVLDVASTEVAMNIAAQLQDELRAPFELRRHRLVVTATVGLTCM
                     DTGQQRAEDVVREADIALSVAKNQESNKVVLYSPNMAGQAANLVSLEADLHVALEKHE
                     LRLLFQPIVDLRSYRMVGAEALLRWRHPVESVLAPDRFLRIAEEAGLMVPITRWIILR
                     VIKLAGEWLRRLPANQSFFISVNLSPTALRDPGLGDYVSSLLRETQLPPSLLKFELTE
                     AALISNVGAARETLDRLHSMGIQLMLDDFGTGYSSLSYLQLFPFDFVKIDRPFVNRGG
                     ADQANTGMMAAMVQMAGSLNLTAIAEIIETEAAAKALQEMGCDFGQGYFFSEPIEAEL
                     ALQRLRTQQAFEPQLTSETVMVPPLEEDDSPTIMIPASTLGFPPDESS"
     gene            complement(5343..6104)
                     /locus_tag="VFB37_08060"
     CDS             complement(5343..6104)
                     /locus_tag="VFB37_08060"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22448.1"
                     /translation="MNVHAIRAIYGFELARTWRTLFQSIASPVLSTSLYFIVFGAAIG
                     SRMAHIDGVSYGAFIVPGLIMLSILTESVSNASFGIYMPKYSGTIYEILSAPISTAEI
                     VIGYVGAAATKSVILGTIILVTARVFVVFTIAHPLWMIASLILTSVTFSFFGFIIGVW
                     ADGWEKLQIVPLMIVTPLTFLGGSFYSISMLPPVWQKVALFNPVVYLVSGFRWSFYGI
                     SDVNIVLSFAMTVVFLLLCMAAVQFIFRTGYKLKA"
     gene            complement(6101..7027)
                     /locus_tag="VFB37_08065"
     CDS             complement(6101..7027)
                     /locus_tag="VFB37_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22449.1"
                     /translation="MPPIISVSRLTKTYASGFQALKSIDLEIRQGEIFALLGPNGAGK
                     TTLISIICGIVNPTAGQVLADGHDIRREFRAARSRIGLVPQELTTDAFESVWDTVAFS
                     RGLFGKPANPAYLERVLKDLSLWEKRNSRILTLSGGMKRRVMIAKALSHEPRILFLDE
                     PSAGVDVELRRAMWDMVRSLRSSGVTIILTTHYIEEAQEMADRIGVIHRGELILVEEK
                     TALMRKLGKKKLTLQLRSALTQIPAALAGSQLELSADGLELVYSYDTQGERTGITALL
                     GRLAEQGIAFKDLQTNESSLEDIFVSLVRARQ"
     gene            7094..7342
                     /locus_tag="VFB37_08070"
     CDS             7094..7342
                     /locus_tag="VFB37_08070"
                     /inference="COORDINATES: protein motif:HMM:NF018500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HZO22450.1"
                     /translation="MTPRDANDEAVFIPHTELAPDTLRRLVESFILREGTDYGEREVS
                     LRQKLAQVYRQLDRGEARIVFDPGSESVDIIVVNPSAR"
     gene            complement(7361..>7805)
                     /locus_tag="VFB37_08075"
     CDS             complement(7361..>7805)
                     /locus_tag="VFB37_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZO22451.1"
                     /translation="AMGVLYALGGGACWALYIVFGQRAGARHGAQTVALGSLISAVVV
                     VPVGIASAGSALLSRSVIVPGIAVAVLSTALPYSLEMVALTRMPARTFGILMSMEPAF
                     AALLGFVYLGERLTSIQWAAIGLIIAASIGTATTSKQELAAPLND"
BASE COUNT         1412 a         2508 c         2501 g         1384 t
ORIGIN      
        1 gtgcgcttcg gcgcctccgg gcacaactcc gtggtcagca acaacgagtt cagggacgac
       61 aaggatgcgg gtctgtgggc ggtgcgcagc gccccggact ctcacgatga catcatcggg
      121 attcacgaca ataaattcgt cgaggacggg accggaatcg tggccggcaa cattccggtg
      181 ctggtcgagc gcaacgactt cgtcaatgcc cacgaggccg acgtgcacgt cgtgggcgcg
      241 ggcgcggtgg ttcgcgggaa ccgcatcaac ggcggcgcat ccatgggcat cgttgcggaa
      301 aacgcgcgcg gtgcggtcat agacgacaat gagctcgagg ggctcacggc gtacggcgta
      361 atggtacgcg gctcctccaa cacgctggtc cgctcaaacc gcctgcacaa ttgcggttac
      421 ggtctcgcct tcgtgctggg cgactcgcat ggggtcagca cggccgtgga caacacgatc
      481 atcgagccga agttcaacgg catcgacgtg atcggcgatt cgccgatcct gcgccgtaac
      541 caggtcctgc ggccccacgc tttcgcacta cacgtggagg acttccagcc acccaacggt
      601 cagaaagtgc agtcgcagcc atttctggac aataacaact tcggcaatag cccggtcagc
      661 agaagcagta ctgccaccgc gatggcgccg tccatgatgc gctgacaggg agagggagtg
      721 aggccgaacg tcgacagcac gcagctcatt ccgcggccgg tatgggagaa cccgcgcgtc
      781 cacctgctgg atgacatctg gctgctgacg atcgttgcga tcctggtcgc caccggcgtg
      841 ccgtggttcg cgagcggctt cgaggtcgag gtcggcagcg cgagctgggg attgctcgcc
      901 cttggaggca tccacgtcgc cttcacgatg cttgcctccc ccacccactc gcccggccat
      961 tggcgcgatc gcgcgctcac cattctggac gtcgccggcg tgatcctgat cggcttcatc
     1021 tggcagcacg tcggggcgtt gcagaacccg atgttcctga ccgtgtttgc cctgccggtc
     1081 atgggcgcca ttttcctctc acgctggcat ccctatctca tcgcgaccgt gagcctgctc
     1141 gtggtcgccg tggtggcgat gatccaggcg cccgaattgc gctggtacgc gagtgggttg
     1201 atcggcaacg aggactggct gacacggctg ttcggacagc agagcgcggc gccgcaatct
     1261 tccttttcag gtttctacgc accctcgggc tatcttgtcg tgctgctgga ggtctttgcc
     1321 gtcatcctgt tcgcctgtgc ggtcgccgcc gagtacgtag gcaccatctt cgagcgcctc
     1381 aacgcgcaca tcgtgatggc gcggaccgag gccgaacgcg gccaggagct ctgggcagat
     1441 ctcatcgagc ggctgccgct accggcgctg ctggtcgatc cggacacgct gcgggtggtc
     1501 gcctcaagca acttcgccat cgcttatctg agtaccggcg agatgccgct cgagggccgc
     1561 aatcttttcg aggcgctgca gttctcctat cccgacatcg tgcaggagct gatcgtgggc
     1621 tccgatggcg gcgcaccgct gacattcctg cgcatcgagg atcagcttcg cgtcacacag
     1681 gtgcgcgtct tgcatgtcgc ccacaagggc cggcggctgg cattgctgac gatcgaggat
     1741 gccaccgagg tcttctgcct gagagctgtc ctggacacct ccgaatatgc cgccgtggtg
     1801 atcgatgccg gcggacggct tctcgcgttc aacaagccgg cgctcgccct gttcggcgga
     1861 gcggaggtcg gcatggatgc ggccgagttg ttgccgcagc ccgagacggg gttgcggtgg
     1921 tgggagcctg gactgaccgg acgacgcaag atgcacctgg agatcggccc gcgcatctat
     1981 caggtgacga gctcggccat cgcattgatg ggcgaggagg agcgcatctt caccgcctca
     2041 ttcctgccgg tggcaaaggc cggcacgacg gatcctttcg gaaccagctc gaccatggtc
     2101 acgagcacat tggggcaggt gagatgagac gggtaaaaag cgcgatgttc atgttgacgg
     2161 tcggcggcct gctgtcaggg ggtgccttcg caagcgacga tcggatcgct gtcagcggcg
     2221 atggcgcctc gctcaccggc accgatggag gctttggcgg ctcgctcggc tggttgcaca
     2281 acttcgatcc ggacacgctg ctgggcattg cgggcgagca tcagaagctg ggtccgtcac
     2341 agtggagctt cggctccttg accggagcaa tgaccaacgg cgagggcgat cagcgttaca
     2401 cggtctcggg cgaggtccac gagggcgccg gtgtcgacac tcacaagcat ttcaattacg
     2461 ccatcgaggc ggtcgggttg acggggacct attaccatcg tctcagcgcg cagctggaag
     2521 acaagcagat caacgtggaa accacgcacg gcaaccttcc caaggccggc ctgtcgtatc
     2581 tgtggaatcc gcactttcag tcgcagatct cctactcgta ctccttcggc ggcaacctcg
     2641 gcacgcggct caccgctgca cgcatcgaca tattcggcaa gcacgtgaac ttcctgaccg
     2701 ggggtgcatg gggtactgcg tcgccgattg tgctgggcgt gcagactatc ctgcccccac
     2761 gccagacgaa ggaaggctac gtcggactgg cgatccccgt gcccagcctg cgcagcgagg
     2821 tgaccctcat cggggattat ctcttcataa aaggcggcgt gatctcggcc ggtgttgtgc
     2881 cggacagcac caagtggacg ggtacgctca actgggtctt ccacgtgggc ggcaagaagt
     2941 gacccgactc acgcgcaagt ccgagctggg atggctgccg acgggcgtcg tcctgctggt
     3001 cgtgctgctg gccggctcgc gactgatcta cctgagcgtg cagcatcatg ccgccgccgc
     3061 gcgacagagc gcggccgcca ttgccgccaa tttcgtcaag aggatcgagc cgcgactctc
     3121 caatctcgcc tatcacgcgg cacaccaggc cgcgctcgcc aataaagttc tgtccggtcc
     3181 caacgcttcg ctccagtcca caccgctcgc gccccacacg ttctggatga ctccggatga
     3241 caaggtgctg ggcgcgcgcc gtggcgagat gcccactgcg agtggcatcg ccagcgaatg
     3301 gcaatcggcc gactccaacc gtcccgtcgc cggctcctcg cttctcggtc cgatgcgcct
     3361 ggggagcgta tggctgctgg ccacgcgcgt accggtcgtg gcgctcgacg agtcgcagca
     3421 gccgcagacc cgcggctggt cgatcgccta cgcggatctg gatgagatca tcgcggacag
     3481 ccatctggcg cgcctggtcg atctgggtta cgagttctcg ttgtctcagg tcgagccgcg
     3541 cagcgcgcgc tcgcgcatct tcgtcggctc ggtcaccgat ccgctgacag accctgtgac
     3601 cacgcggatt ccgttgccca gcggggtctc cccgaccatt gccggcagct acctcgagct
     3661 tgcgatccag ccgcgtagcg gctggtatcc ggccaccgag ctcgcctccg acatcggcct
     3721 gctggcattc ctcgcctggc tgctcgcctt cggaacgcac gatctgagtc acgcgctcga
     3781 gcgctcgcgc gccgcgctga agggtgcgcg gcggcgcttg cgagcgatcc accatcagct
     3841 cgcgaccgag atgcagcagc gcttgagcct gcaggagaca ttcgatcacg cgcggttcca
     3901 cgatgccttc accggcctgc caaatcgccg ctatttcatg gaccagctcg atcgcgcgct
     3961 gcgcgaggtg cgcaccaagc gccgccagcg catcgcggtc atcatcgtgg acattgcccg
     4021 gttcaaactc atcaacgaca tgctgggtca caccgccggg gatgagctga tggtccaggc
     4081 ggcgcgacga ttcgagaagg cggccaagac attcgaaggc gtgctcgcgc gctggggcgg
     4141 agatcagttc gcgctcctgg tgctcgatgt ggcttccacc gaggttgcaa tgaatatcgc
     4201 cgcgcagctg caggatgagc tgcgtgcacc cttcgagctg cgtcgtcacc ggctggtcgt
     4261 gacggcgacc gtgggcctca cctgcatgga taccgggcag caacgcgccg aagacgtggt
     4321 gcgcgaggcc gacattgcgc tgtcggtggc caagaaccag gaatccaaca aggtcgtgct
     4381 gtacagcccc aacatggcgg gccaggcggc gaacctcgtg agcctcgaag ccgatctgca
     4441 cgtcgcgctg gaaaagcacg agctgcggct gctgtttcag ccaatcgtgg atctgcgcag
     4501 ctatcgcatg gtgggcgcgg aagcgctgct gcgctggcgc catccggtgg agagcgtgct
     4561 ggcgcccgat cgcttcctgc gcatcgcgga ggaagctggc ctgatggtgc cgatcacccg
     4621 ctggatcatc ctccgggtga tcaagctggc gggcgagtgg ctgcggcggc tgcccgcgaa
     4681 ccagagcttc ttcatcagtg tcaacctctc gccgaccgcg ctgcgtgacc cgggcctcgg
     4741 cgattacgtc tcctcgctgc tgcgcgagac gcaactgccg ccctcactgc tgaagttcga
     4801 gttgaccgag gcggctttga tcagcaacgt gggcgccgcg cgcgagacac tggaccggct
     4861 gcacagcatg ggcattcagc tcatgctgga tgacttcggt accggctact catctctcag
     4921 ctacctgcag ctcttcccgt tcgatttcgt gaagatcgat cggccgttcg tcaatcgcgg
     4981 cggtgccgat caggccaaca ccggcatgat ggctgcgatg gtgcagatgg ccgggagcct
     5041 caatctcacc gcgattgccg agatcatcga gacggaggcc gccgccaagg ccttgcagga
     5101 aatgggctgc gatttcggcc agggctattt cttcagcgag ccgatcgaag cggagctcgc
     5161 tctgcagcgg ctgcgcactc agcaggcgtt cgagccacag ctcacctccg agaccgtgat
     5221 ggtgcccccc ctcgaggaag acgactcccc gaccatcatg attcccgcca gcaccctcgg
     5281 ctttcctcct gacgagagct cgtaaggtcc aagcgtctgg atcggcgagt gcggccggtc
     5341 cgtcaggctt tcagcttgta accggtgcgg aagatgaact gcaccgctgc catgcagagc
     5401 agcagaaaga ccaccgtcat cgcgaaactc agcacgatgt tgacatccga gatcccgtaa
     5461 aagctccagc ggaagccact caccaggtac acgaccggat tgaacagcgc aaccttctgc
     5521 cacacaggtg gcagcatgct gatcgagtag aaactgccgc cgagaaaggt cagcggtgtg
     5581 acgatcatca ggggcacgat ctgcagcttc tcccagccat cggcccagac tccgatgatg
     5641 aagccgaaga agctgaaagt caccgacgtc aggatcagtg aggcgatcat ccagagcggg
     5701 tgcgcgatcg tgaagacgac gaacacgcgc gccgtgacga gaatgatggt tccgaggatc
     5761 accgacttgg tcgccgccgc cccgacatag ccgatcacga tttcggccgt ggagatcggc
     5821 gccgagagga tctcgtagat cgtgccggag tacttcggca tgtagatgcc gaaggaggca
     5881 ttggagacgc tctccgtcag gatcgagagc atgatgaggc cgggcacgat gaaggcgccg
     5941 tagctcacgc cgtcgatgtg cgccatgcgc gagccgatgg cggcaccgaa cacgatgaag
     6001 tagagggaag tggacagcac cggcgaggcg atgctctgga acagcgtccg ccaggtgcgc
     6061 gccagctcga acccgtagat ggcgcgaatc gcgtgcacgt tcattgccgc gccctcacca
     6121 ggctcacgaa gatgtcctcg agcgagctct cattcgtctg caggtccttg aaggcgatgc
     6181 cctgctcggc caggcgtccc agcagcgcgg tgatgccggt gcgctcgccc tgcgtgtcgt
     6241 aggaatacac cagctccagc ccatccgcgg agagctccag ctgggagccg gccagcgccg
     6301 ccggaatctg cgtgagggcg ctgcgcagct gcaacgtcag tttcttcttg ccgagcttgc
     6361 gcatcagggc cgtcttctcc tccaccagaa tcagctcgcc cctatggatc actccgatgc
     6421 ggtcggccat ctcctgcgct tcctcgatgt agtgggtcgt cagaatgatc gtgacgccgc
     6481 tgcttcgcag cgagcgaacc atgtcccaca tggcgcgtcg caactcgaca tccacgccgg
     6541 cgctcggctc gtccagaaac agaatgcgcg gctcatgcga aagcgccttg gcaatcatca
     6601 cgcgacgctt cattccgccc gacagtgtga ggatcctgga gttgcgcttc tcccagagcg
     6661 acagatcctt cagcacgcgt tccaggtaag cagggttcgc aggcttaccg aacaggcccc
     6721 ggctgaaagc gaccgtatcc cagacgctct cgaaggcatc ggtggtgagc tcctggggga
     6781 caaggccgat cctggagcgc gccgcacgga actcacgccg gatgtcatgg ccgtctgcca
     6841 gaacctgccc ggcggtcggg ttgacgatgc cgcagatgat gctgatgagt gtggtcttgc
     6901 ctgcaccgtt cggacccagc agcgcgaaga tctccccctg gcggatctcc agatcgatgc
     6961 tcttgagcgc ctggaatccg ctggcgtagg tcttcgtcag gcgcgagaca ctgatgatgg
     7021 gcggcaaggt cgctcccggg aaagcgcagg aatgcgatat tgttgcccac cgggagccgc
     7081 gattgggtac aaattgacgc ctcgcgatgc gaatgacgaa gcggtgttca ttccgcacac
     7141 cgagctcgcg cccgatacgc tgcggcgcct cgtggagtca ttcattctgc gcgaagggac
     7201 cgactacggc gagcgggagg tgtccctgcg gcagaagctt gcgcaggtgt accggcaact
     7261 cgacaggggc gaggctcgca tagtgttcga ccccggctcc gagagcgttg acatcatcgt
     7321 ggtgaatccg tcggcgcgct gagcgccgcc gggcagggct tcagtcattc agcggcgccg
     7381 ccagttcctg ttttgacgtc gtggcagtgc cgatcgaggc cgcgatgatc agcccgatcg
     7441 cggcccattg aatgctcgtg agccgctcgc ccagatagac gaagcccagc agggcagcga
     7501 aggcaggctc catgctcatc agaatgccaa aagtgcgcgc aggcatgcgc gtgagtgcga
     7561 ccatctccag cgagtagggc agagccgtcg agagcacggc gacggcaatc ccgggcacga
     7621 tgaccgagcg cgacagcagc gcggatcccg cgctggcaat gccgacaggc accacgacga
     7681 ccgcggagat cagactgccc agcgccaccg tctgtgcgcc gtgccgcgct ccggcgcgct
     7741 gcccgaacac gatatagagc gcccagcagg cccctccacc cagtgcgtag agcacaccca
     7801 tggca
//
LOCUS       DASRHN010000211         6462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_563305, whole genome shotgun sequence.
ACCESSION   DASRHN010000211 DASRHN010000000
VERSION     DASRHN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563305"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /gene="lon"
                     /locus_tag="VFB37_08080"
     CDS             complement(<1..1784)
                     /gene="lon"
                     /locus_tag="VFB37_08080"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZO22452.1"
                     /translation="MESVPVLPLRDVVVYPHMVIPLFVGREKSIQALDVAMRTDKRIM
                     LVAQKQADVDDPKADDLYRIGTVATILQLLKLPDGTVKVLVEGVDRARIEKLHAGEYY
                     SSDLTLLPDHEAYDEREMDVLARSVISQFEQYVKLNKKVPPEVLTALAGIEHSGRLAD
                     TVAAHMSLKLAEKQKVLEILDIRKRLEHILVAIEGEMDVLQIEKRIRGRVKAQMEKSQ
                     REYYLNEQMKAIQKELGEMDEGGNEITELEQKIAKAGMPKEAREKATGELNKLKMMSP
                     MSAEATVVRNYIDWLVKVPWKKRSKIIKDIARAEKVLDEDHFGLEKVKERIVEYLAVQ
                     QRVDKLRGPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGGVRDEAEIRGHRRTYI
                     GSMPGKIVQNMAKAAVHNPLFLLDEVDKMSMDFRGDPSSALLEVLDPEQNSTFNDHYL
                     EVDLDLSEVMFVCTANSLNIPAPLLDRMEVIRLPGYTEDEKMNIARRYLVPKQMKNNG
                     VQPEELKVSDAALLDIVRYYTREAGVRNLEREIAKIARKAVKQMLLHKKEQKSVSVMP
                     RNLDKFLGVRRFRYGKAEDLNRVGQVTGL"
     gene            complement(1971..3290)
                     /gene="clpX"
                     /locus_tag="VFB37_08085"
     CDS             complement(1971..3290)
                     /gene="clpX"
                     /locus_tag="VFB37_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZO22453.1"
                     /translation="MSEDSRGRHDDGKLLYCSFCGKSQHEVRKLIAGPSVFVCDECVE
                     LCNDIIREELEDRAERARDKLPKPHEIKKVLDEYVIGQERAKKVLSVAVYNHYKRLQT
                     RTSGSRSKDDVEIAKSNILLIGPTGCGKTLLAETLARLLNVPFTIADATTLTEAGYVG
                     EDVENIIQKLLQKCDYDVEKAQTGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLK
                     LIEGTIASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLEKIILNRTQKGGIGFTS
                     EVRDVSTRPHGSDLFHDVEPEDLIKYGLIPEFVGRLPVVATLDELDETALVSILTEPK
                     NALTKQYRRLFEMEGSELEFRDEALKAVAHRAMQRKTGARGLRTILETVLLDTMYELP
                     SMRNVAKVVIDETVIEGQTKPYLVYGSDEARLASVDEPRRASGTNHH"
     gene            complement(3438..4061)
                     /gene="clpP"
                     /locus_tag="VFB37_08090"
     CDS             complement(3438..4061)
                     /gene="clpP"
                     /locus_tag="VFB37_08090"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZO22454.1"
                     /translation="MNERGLNLVPMVVEQTARGERAYDIYSRLLKERVVFIVGAVDDY
                     MANIVVAQMMFLESENPDKDIALYINSPGGVVSAGLAVYDTMQFIKPDVSTICIGQAA
                     SMGAVLLAAGAPGKRFCLPHARVMIHQPLGGFQGQAADMEIHAKEMLETRDRLNKILA
                     KHTGQSVDRIKQDTDRDRFMDWNEAVRYGLVDRVLEKRGEVAALPSK"
     gene            complement(4073..5404)
                     /gene="tig"
                     /locus_tag="VFB37_08095"
     CDS             complement(4073..5404)
                     /gene="tig"
                     /locus_tag="VFB37_08095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZO22455.1"
                     /translation="MQVSVTATGGLQRRLEVAVPANEVDSEVEQRLKSISRTARLKGF
                     RPGKAPITVVRKQFGPQVHQEVITDLMRSSFAQALTQEKLTPAGGPRIEPITVGPGTE
                     LRYAAVFEVLPEIRVESPERITIERPQAEVTEDDVTAMLESMRRQRPIFTAVDRPARD
                     TDRVTIDFDGRIDGQPFEGSEGRDVQIVVGAHQALPQLEEGVQNASAGESRSVTLPFP
                     PEHPNKGVAGQTAQLQVTIKRVEEQSLPEVDEAFCRAYGVEEGGIAALRVEVRKSMER
                     ELQDIIRNRIRVQVMDGLYRENTLEVPRTLIDEQVQRMQLDAARRMGAKDVSQVPPRE
                     PFEEPARRRAALGLLMGQIVQSEDIKLDQARVHSRLGDIIANYPDPDEARRAYAQNAD
                     AMRQIESAVLEDQVVDWVVAHARVTERPMTFKELTGFGQDAGTPGEETGSQ"
     gene            complement(5515..5596)
                     /locus_tag="VFB37_08100"
     tRNA            complement(5515..5596)
                     /locus_tag="VFB37_08100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5560..5562),aa:Leu,seq:tag)
     gene            complement(5742..6002)
                     /locus_tag="VFB37_08105"
     CDS             complement(5742..6002)
                     /locus_tag="VFB37_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22456.1"
                     /translation="MVSRKSESRPRIIRTQVQEVVLHLRQIQSAIVVAVAALRQQNSD
                     LDEDIANVLQRSVADRIQDQIERLEAVTRLLASLGRGARPAA"
     gene            6168..>6462
                     /locus_tag="VFB37_08110"
     CDS             6168..>6462
                     /locus_tag="VFB37_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO22457.1"
                     /translation="MADRRARQASGGFALRLRQVIEAHGSANSIARTIERSEGAVRKW
                     LRGESEPNVTDLRALCEHTNTSIEWLVSGRGRPDGAPVSLQESAPPAYGQPAGG"
BASE COUNT         1115 a         2102 c         1936 g         1309 t
ORIGIN      
        1 agtccagtga cctggccaac gcggttgaga tcctccgcct tgccgtagcg aaagcgccgc
       61 acgccaagga acttgtccag gttgcgcggc atcaccgaca ccgacttctg ctccttcttg
      121 tggagcagca tctgcttcac ggccttgcga gcgatcttgg cgatctcgcg ctcgagattg
      181 cggacgccgg cttcgcgcgt gtagtaacgc acgatgtcga gcaacgccgc atccgatacc
      241 ttcagctcct cgggctgcac gccgttgttc ttcatctgct tgggcacgag gtagcggcgc
      301 gcaatgttca tcttctcgtc ttcggtgtaa ccgggcagac ggatgacttc catgcgatcg
      361 agaagcggtg cagggatatt caggctgttg gcggtgcaga cgaacatcac ctcggagaga
      421 tcgagatcga cctccaggta atgatcgttg aaggtggaat tctgctccgg atcgagtact
      481 tcgagcagcg cggaagaagg atcgccgcgg aagtccatcg acatcttgtc cacttcatcg
      541 agcaggaaca gcggattgtg cacggccgcc ttggccatgt tctgcacgat cttgccgggc
      601 atggagccga tgtacgtacg acggtggccc cggatctcgg cctcatcccg cactccaccc
      661 aatgacatgc gcacgaactt gcgattcgtg gcgcgcgcga tgctctgccc gagtgaggtc
      721 ttccccaccc cgggtggccc cacgaggcac aggatcggac cgcgcagctt gtccactctc
      781 tgctgcacgg ccagatattc gacgatgcgc tccttgacct tctccaggcc aaagtggtcc
      841 tcgtccagca ccttctcggc gcgcgcgatg tccttgatga tcttggagcg tttcttccac
      901 ggcactttga ccagccagtc gatgtagttg cgcacgaccg tcgcttcggc ggacatgggc
      961 gacatcatct tcagcttgtt cagctcaccc gtggccttct cgcgcgcttc cttgggcatg
     1021 ccggccttgg cgatcttctg ctcgagctcg gtgatctcgt taccgccctc gtccatctcg
     1081 ccgagctctt tctgaatggc cttcatctgc tcattcaggt agtactcgcg ctggctcttc
     1141 tccatctgcg ccttgacgcg gccgcgaatg cgcttctcga tctgcagcac gtccatctcg
     1201 ccctcgatgg ccacgaggat gtgctccagg cgcttgcgga tatcgaggat ctccaggacc
     1261 ttttgcttct ctgcgagctt cagcgacatg tgggcagcaa cggtgtccgc cagcctgccg
     1321 gaatgctcga tgcccgccag cgccgtgagc acctcgggcg ggaccttctt gttcagcttg
     1381 acgtactgct cgaactggga gatcaccgag cgagcaagca cgtccatctc gcgctcgtcg
     1441 taagcctcgt gatcgggcag cagcgtgaga tcggaggagt agtactcgcc ggcatgcagc
     1501 ttttcgatgc gggcgcggtc gaccccttcc accagaacct tcacggtgcc atcgggcagc
     1561 ttcagcagct gcaggatcgt ggcgaccgtg ccgatccggt agaggtcatc cgccttcgga
     1621 tcatcgacat cggcctgctt ctgggccacc agcatgatgc gtttgtcggt gcgcatggcg
     1681 acgtccagag cctggatcga cttctcgcgc ccgacgaaca gcgggatcac catgtgcggg
     1741 tagacgacca cgtcgcggag cggcagaacc ggcacgctct ccaccgcggt gacgctcgga
     1801 ttggcagggg taggatcgga cacgctcaag ccctcgatgg cggatggaac aagatctccg
     1861 ctgcgagcga agaccggccg gggagaaggc gctgcaagac tagtctagcg ggaaatgagg
     1921 ttgcgccgcc gcgtttcaac ggcagcgcca cagtccctga acgggaacag tcagtgatgg
     1981 ttggttccgg atgcgcgccg cggctcatcg acgctggcca gcctggcctc gtccgagccg
     2041 tacaccaggt agggtttggt ctgcccttcg atgaccgttt catcgattac gactttagcg
     2101 acgtttcgca tcgaaggcag ctcgtacatt gtgtcgagga gcacagtttc gagaatcgtg
     2161 cgcaaacctc gcgcccccgt cttgcgctgc atcgcccgat gcgcgaccgc cttcagcgcc
     2221 tcgtcacgga actcgagctc cgacccctcc atctcgaaca gccgccgata ctgcttcgtg
     2281 agtgcgttct tgggctcggt caggatcgag acgagagccg tctcgtccag ctcatcgagc
     2341 gtcgccacga ccggcagccg acccacgaac tcaggaatga ggccgtactt gatgaggtct
     2401 tcgggctcca catcgtgaaa cagatcactg ccgtgcggac gcgtgctcac gtcgcgcacc
     2461 tcggaggtga agccgatgcc gcccttctgg gtccggttca ggatgatctt ttcgagtccg
     2521 gcaaaggcac cgccacagat gaagaggatg ttcgaggtgt ccacttgcag gaattcctgc
     2581 tgcgggtgct tgcggccgcc ctgcggcggc acggaggcga tcgtcccctc gatgagcttg
     2641 aggagtgcct gctgcacgcc ctcacctgac acatcgcgtg tgatggaggg gttgtccgac
     2701 ttgcgcgaga tcttgtcgat ctcgtcgatg tagacgatgc cggtctgggc cttctcgaca
     2761 tcgtaatcgc acttctgcag cagcttctgg atgatgttct cgacgtcctc accgacgtag
     2821 cctgcctcgg tcaacgtggt ggcatcggcg atggtgaacg gcacgttgag cagccgggcg
     2881 agcgtctcgg ccagcagagt cttgccacag cctgtcgggc cgatgagcag gatattgctc
     2941 ttggcaatct ccacatcgtc cttcgagcgc gagccggagg tacgggtctg tagccgcttg
     3001 tagtggttgt aaaccgcgac cgagaggact ttcttggcgc gctcctgacc gatcacatac
     3061 tcgtccagga ccttcttgat ctcatggggc ttcggcagct tgtcgcgcgc acgctcggcg
     3121 cgatcctcga gctcctcgcg gatgatgtca ttgcaaagct cgacgcattc gtcgcaaacg
     3181 aataccgacg gaccggcaat cagcttgcga acttcgtgct ggcttttccc gcagaacgag
     3241 cagtacagga gctttccatc atcgtgacgg ccgcgggaat cttcactcat cgaaggacct
     3301 cataatcgcg cggcgcagca ggcgcggcgc aacttcttat agcatggcgt ccggccgcga
     3361 agcaaaacag cgcatgggct cgtaagcctt tgtgtggata gggattgtaa cccctcccac
     3421 gcccttcgcc cagcgcctca cttcgatggc agtgccgcaa cctctcccct cttctcgagc
     3481 acccggtcta ccagcccgta ccgcaccgct tcgttccaat ccatgaagcg gtcgcgatcg
     3541 gtgtcctgct tgatgcgatc gacgctctgg ccggtgtgct tcgccaggat tttgttcagt
     3601 cggtcgcggg tttcgagcat ttctttggcg tggatctcca tatcggccgc ctggccctga
     3661 aatccgccga gcggctggtg gatcatcacg cgggcgtggg gcaggcagaa tcgcttgcca
     3721 ggggccccgg cggcgagcag gaccgccccc atgctggcag cctggcctat gcagatggtg
     3781 ctgacgtccg gcttgatgaa ctgcatggtg tcataaacag ccaggcccgc gctcacgacg
     3841 ccgcccggcg aattgatgta cagggcgatg tccttgtccg gattctcgga ctcgaggaac
     3901 atcatttgag ccacgacaat atttgccatg tagtcgtcga cggctccgac aatgaatacc
     3961 acacgctcct tcagaagacg cgagtagatg tcataagcac gttcgccccg agcagtttgc
     4021 tcgacgacca tcggtacaag gttcaatcca cgttcgttca tagtcactct cgctattggg
     4081 aacccgtttc ctcgccgggg gttccagcat cctgtccgaa cccggtcagc tccttgaaag
     4141 tcatgggccg ctccgtgacc cgcgcgtgcg cgacgaccca gtccacgacc tgatcctcca
     4201 ggaccgcaga ttcgatctgt cgcatggcat cggcgttctg ggcataggcg cgccgcgcct
     4261 catccgggtc cgggtaattg gcaatgatat cgcccagccg gctgtgcacc cgtgcctggt
     4321 cgagctttat gtcctcactc tgcacgatct gccccatcag caatcccagg gccgcccgcc
     4381 tgcgcgcagg ctcctcgaac ggctcccgcg gcgggacctg gctcacatct ttggcaccca
     4441 tgcgccgcgc cgcatcgagc tgcattcgct gcacctgctc gtcgatcagc gtacgcggga
     4501 cctccagcgt gttctcccgg taaagaccgt ccatcacctg cacgcgaatg cgattgcgaa
     4561 tgatgtcttg cagctcccgc tccatgctct tgcggacctc gacccgcagg gcggcgatcc
     4621 cgccttcctc cacaccgtac gcgcggcaaa aggcctcgtc cacttccgga agggattgtt
     4681 cctccacccg ctttatggtg acttgcagct gcgcggtctg gcccgcaaca cccttgttgg
     4741 ggtgctccgg cggaaaagga agggtgacgc tgcggctctc tcctgctgag gcgttttgca
     4801 cgccttcttc cagctggggc agcgcctgat gagcgccaac cacgatctga acatcccgcc
     4861 cctcgctgcc ctcgaagggc tggccatcga tgcgcccatc gaaatcgatc gtgacccgat
     4921 cggtgtcgcg ggcagggcga tcgacggcgg tgaagatggg gcgttgtcgc cgcatgcttt
     4981 cgagcatcgc ggtcacatca tcctcggtca cctcggcctg cgggcgttcg atcgtgatcc
     5041 gctcggggct ttccacgcgg atctcgggga gcacttcgaa caccgccgca taacgcaatt
     5101 ccgttccggg acctaccgtg atcggttcga tccgcggtcc accggccggc gtgagcttct
     5161 cctgggtcaa cgcctgcgcg aacgagctgc gcatcagatc ggtgatcact tcctgatgca
     5221 cctgcgggcc gaactgcttg cgcactaccg tgatgggcgc cttgccgggg cggaagccct
     5281 tgagccgcgc cgtgcgggag atgctcttca ggcgctgctc gacctcggaa tccacctcgt
     5341 tcgcgggcac ggctacttcc aggcggcgct gcagaccgcc cgtggcggtc accgatactt
     5401 gcatgggcaa atcctcgtct aaattcctgc tcgtcccggc caggcctgct gagcccgcgg
     5461 gccgaccgca gcttcagcga agcgcatcga atcgcacgcg aagcgcgcgt gatatgcgaa
     5521 aggagagact cgaactctca cgggtttccc cactggaacc taaatccagc gcgtctacca
     5581 gttccgccac tttcgcccgt gcagcgcgcg caccgaacgt gcggcttcag gcagcgctat
     5641 tcgactgatg ctggcgtact tttggggctt cggaagtata cccgatgaag ctctcgagca
     5701 cgcccggggg gctctgtcga cctcggttat cggggcgccc ttcaggcggc cgggcgggct
     5761 ccccggccga gggaggcgag cagccgcgtg accgcctcca gccgctctat ctgatcctga
     5821 atccgatcgg cgacgcttcg ctgcagaaca ttggcaatgt cctcatcgag gtcactgttc
     5881 tgctggcgca gcgccgcaac cgccacgacg atcgccgatt gaatctgacg caggtgcagg
     5941 accacctctt gcacctgggt acggatgatg cggggcctcg actcggactt gcgggaaacc
     6001 atgctcgctc cttgtctccc acccagccgc atcacttgcg cccgggcggt acgttacgtt
     6061 actttaatac gagaagtcaa cacttatgtg atggcatttg gctaggtcgg cggctggcgc
     6121 tacatttgtg ccgcacccgc aggtgaatgc actcaggcag atttcccatg gccgacaggc
     6181 gcgcaagaca agcgagcggc ggcttcgccc tgcgtttgcg acaggtgatc gaggcgcatg
     6241 gcagcgccaa ttcaatcgcc agaaccatcg agcgctcgga aggcgcagtg cgcaagtggc
     6301 tgcgtggcga atccgagccc aacgtgaccg acctgcgcgc gctgtgcgag cacaccaaca
     6361 ccagcatcga gtggctggtc agcggccggg gccgcccgga tggcgcgccg gtctcgctac
     6421 aggaatcagc gcccccggcc tacggccagc cggcaggcgg aa
//
LOCUS       DASRHN010000212        24578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2050368, whole genome shotgun sequence.
ACCESSION   DASRHN010000212 DASRHN010000000
VERSION     DASRHN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050368"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFB37_08115"
     CDS             complement(<1..426)
                     /locus_tag="VFB37_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22458.1"
                     /translation="MVVGTVGTQDRGRRRDLQESAPQDVLVARQPIVGMLQDDAERIL
                     GEMLGASPTASAVDVVPPGRWTAASVRHYLHVGEGDGYWDFYKPWTALMLSVTDATYR
                     SDTWVRVEGTDYFKLRVLLSGTLRARSGQVIARAPEALLY"
     gene            complement(408..1256)
                     /locus_tag="VFB37_08120"
     CDS             complement(408..1256)
                     /locus_tag="VFB37_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO22459.1"
                     /translation="MSTNEDIVQGIDHLIVMVRDLDRAERIWQSLGFATTPRGFHRTG
                     GTANHLIMLDRTYIELLGSSGQPSAAAAYSDMMADPGLWGTALRGSAQALFQLWSAAG
                     LQPSTPVDLARGVEIEGRDEVARFRLAMLERTPELPFLLFCCEHVTPQFVWHPGVARH
                     PNGARRLRELIVLAESGATRSSLERVLGRPATGSAGGAVLRLGESRISLLSAASFEQR
                     FRQAPRFGTRREPLLAGFGLASDNLNRAREFAERAGHRVWETVEGGFFVHLPDEGVMI
                     EWSSEQ"
     gene            1452..1898
                     /locus_tag="VFB37_08125"
     CDS             1452..1898
                     /locus_tag="VFB37_08125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZO22460.1"
                     /translation="MTGSREGHGTGSPPSSEQIAALLAESPFHQVLGLRLESVDPGAH
                     SIAIRCDFGPRLERSPGTGQYHGGVIASIIDIAGDFALIAVLGHGVPTINFRVDYLRP
                     ARRGGLIARARVRKSGRTVGVVDIDIEDDDGRLIAIGRGCYGTQAG"
     gene            1928..2938
                     /locus_tag="VFB37_08130"
     CDS             1928..2938
                     /locus_tag="VFB37_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZO22461.1"
                     /translation="MRAVLTVSHGGREALQYRNDVPNPQPDAAEALVCVAATSVNYHD
                     IFTRRGMPGVRISLPVIAGSDIAGTVLAAGPRADAAWVGKRVLIDPVLRQSDRIGMLG
                     ETLPGGRAELIAVAQSMLVELPEEVSFEQAASLPLAYGTAHRMMVTRGEVKAGERVLV
                     LGASGGVGVACVQIAKLLGAEVIACASSEAKLQRLREIGADHVINYREQPFQEAIKSL
                     CGKPRVSGAGGVDVAVNFTGGDTLLPTQKCVKLGGRILCCGATAGFNLQLDARYWWTF
                     EQTLIGSDGWMKEDLERLLALVASGRLRPVIDRILPLEQTAEGERLLEDREVFGKVVL
                     KP"
     gene            2952..4538
                     /locus_tag="VFB37_08135"
     CDS             2952..4538
                     /locus_tag="VFB37_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZO22462.1"
                     /translation="MSELLNLGDILRLPALGDRLAYLDLRRPDQPLPLTAEELDERIR
                     AIGRGLVRRGIRLGERVGILAENRVEFLATYLAIMRMGAVAVPVNFKLAGATIAHIMR
                     DSAVKLVFVDAQRTRQVPPGMPIVSFDAAAGKEFEDFLDAGPFETFVPGEQDLAEILY
                     TSGSTGMPKGVPLTHRGQVWALANYCAELSEGEGTPEDRTLVVAPLYHMNGLFFSTVA
                     LANRMAVLSLPRFEVRTYLKTVAEQRCTVLSGIPTMFAMMVRETDLIETLDLSSVKEV
                     AIGSAPLTDALLGQVQALFPNAEVSNGFGTTEAGPAVFGAHPDGLPTPPLSLGYPLAS
                     IGWRLVDGSSSEEGVLALRTPALMGGYVNLPHVTAEKMRDGWYLTGDIMRRDAGGFFY
                     FVGRADDMFVCGGENIYPGEVEKLLERHPGVAQAIVVPAPDDIKGQIPVAFIVPRPSV
                     APSADEIKQYALENGAAYAHPRFVEFRDLLPVSGTHKIDRAALVKEAAQLSRAAGRSR
                     ISLEKSSSTVAAADPHSSRK"
     gene            4581..6146
                     /locus_tag="VFB37_08140"
     CDS             4581..6146
                     /locus_tag="VFB37_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HZO22463.1"
                     /translation="MQLSADDPRAIANVPAGPGRERAWLGYPRGAWLVISVEFWERFS
                     FYGMLAILALFLTDDVAHGGFGWSSASALALVGAYSGAMYAFPAFGGYLADRVLGRRR
                     AVALGATCMLIGQSLTASPAYLPKLLGLWHRQPLLPALRSIGVPLGQLRQSGQLSAAL
                     QMHGAALDPVHGATWLAQAYTASAMGFYAALFFLILGNAVMKSTLVVLCGETFRPDDP
                     RREGAFAYYYLGISVGAMLSGFVVGIASERYGWASGFAVAAIGMAVALSLYLLLSKRW
                     LGNIGEHSAATSDSNTVSATPPAAPAAAETWRRIILVLVLAALMCIFSTGWFQMYGSW
                     SLFIEHSVDRAVGPLTVPVAWFSSINAVVVISIAPILASLWVRLAASNRSVNIIQKFF
                     FAIVMAMLGNGLMYWAGALAGRGQLAPIWMPLLGVSMLGCGEIVAWTATYGFVTRAAP
                     AGFASVTMGAWYLLTLGLGGYLSGVTGNWVDTVGFTATFGIIAIVMGVGAAAALVMRA
                     PLLRLAARARVEL"
     gene            6171..7718
                     /locus_tag="VFB37_08145"
     CDS             6171..7718
                     /locus_tag="VFB37_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO22464.1"
                     /translation="MTNNHGISRRRFLAQSSAATASAALSLGWRRAHSRQKDNAAQLT
                     SLSAVKAVAALRNGDITAEAYAGALLLQAQSGALLNAFITLRPEAVLEAARDCDRRRR
                     AGQPLGPLHGLPIPIKDSVNTRDLPTTAGTPALRHFQPAADAPTVAILRRAGAIVLGK
                     TNLHELSYGYTSNNHAYGAVRNPYDPTRIPGGSSGGTAAAIAYHMAPLGIAEDTEGSI
                     RVPAALCGLAGFRPTTGRYSTAACAPITPLFDQLGPHARSVEDLLLFDGVVTGDVQPP
                     ASASLKGVRLGLIRSFYWSGLDPEVERITDAALKRLTDAGIELVEAELPELPHLIALT
                     TDPIQNHDVKPSLTRYLAEYHTGVTFESLLAQASPDVREMIEPAITPGGPDYVPEAQY
                     RAAVNVHLPALRRLFKDCFARAQISAIVLPTTILPAPKLGEENMEVEVRGRRMPLDVA
                     IARNIAPGSTAGLPGLVLPAGLTPNGLPVALQFDGPSGSDRALLSLGLGLESALGRLA
                     PPPKLPG"
     gene            complement(7744..8109)
                     /locus_tag="VFB37_08150"
     CDS             complement(7744..8109)
                     /locus_tag="VFB37_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO22465.1"
                     /translation="MITHVKFVGIPTRDQDRALKFYTEQLGFEVSTDQPFDAHQRWIE
                     LRVAHSQTRVVLFTPEGHESRIGTFFNGSLACDDVEATYRQLRQRGVEFTGEPQRQPW
                     GTFATFKDPDGNQFVLSSS"
     gene            8194..8490
                     /locus_tag="VFB37_08155"
     CDS             8194..8490
                     /locus_tag="VFB37_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO22466.1"
                     /translation="MQRLSIDAYVTDVLMPDLVGHDRRPAAFVVYLFLLRHSGQRSSD
                     RVSASLQTIATRTGLSKSSVQSSIRHLKRRRLLDPDLKATVTEPVRRVLRPWLR"
     gene            8609..9085
                     /locus_tag="VFB37_08160"
     CDS             8609..9085
                     /locus_tag="VFB37_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22467.1"
                     /translation="MAGRIEYQTMNRFPIFILQTLVILTSSMWPATLLAAPPVALYEV
                     TTEIGMPHLEENLRYTITHERRCLSRDGLHRLFPILQHPALAGCYLGAPAISSAHEVT
                     YPLLCDGNHGTTGVARWEAGYRRLTGTLAVKLGGKNMTFFQRVTAIALGGCKASTP"
     gene            complement(9127..9780)
                     /gene="dsbG"
                     /locus_tag="VFB37_08165"
     CDS             complement(9127..9780)
                     /gene="dsbG"
                     /locus_tag="VFB37_08165"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HZO22468.1"
                     /translation="MNIPGSMRQALAIGTPIRHLATASRRVVLLAAAAICLFYAGSGA
                     AAPPPWSPDPGTPDVWLRLQRASWAAQGSTHPQHILYVITDANCPFCHDLWLSLQPLY
                     AENLQVRYIMVGILARNSPGKAAAILEAKNPSQALDRNERRWASLPDDLGGGIPPLRT
                     PRPATLAAIASNEALMHDLGARGTPALIYRDTAHAMHVVQSSLDAGKLKVIIAASST"
     gene            complement(9781..10641)
                     /locus_tag="VFB37_08170"
     CDS             complement(9781..10641)
                     /locus_tag="VFB37_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HZO22469.1"
                     /translation="MIVHLIDGTYELFRHFYGLRRFNKGQDRPFGAVNGVLHTILQMI
                     EEGATHLGVATDHVIESFRNDLWAGYKTGEGIEPALLAQFHPLERGLAAMGVTVWPMI
                     ELEADDALASAARLAGADACVQKVCIWTPDKDLAQCVVEDRIVQVDRKTRVIRNAQAV
                     RQKFGVEPRHIPDFLALVGDTADGYPGLSGIGAVTAARLIERYGAIEAFPPEILGERR
                     ELALRFKQLATLKSEARLFATVDELKWRGPTLGFAACAAEFADARLVPRTLRAASGES
                     VRTNVGVRGD"
     gene            10797..12515
                     /locus_tag="VFB37_08175"
     CDS             10797..12515
                     /locus_tag="VFB37_08175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZO22470.1"
                     /translation="MLFNRRQLLASGLTATAVAATGRFTVAGTPPDHGGSDPQVNARL
                     DQFIEQYMRELRSPGMTLVLADRDGIQRVAAYGFGDLERSRPVRTDELFEIGSISKSF
                     VALCLLQLHDEGKLDLHKPVVDYLPWFRVESRFAPITTHHLLTHGSGLPGNADVFLSD
                     PADRHRAAYAPGEHFHYNNMAFTLLGHLVWTLDGRELPQAIRKRILEPLGMTQTEPAI
                     TLDVRERTVKSYCALQNDRPDSPSMPLSEAPGLIETSGAGCVASTARDMGSYIRMLAN
                     HGAGPNGRLVSKESFALFCQPHIAADEFGPTAHYGYGIAVDRLEGNTVVRHTGGMVSF
                     MSAIIVDLDQGVGAFASINAMQNYRPTPVVQFAALLMRATKLRKNVAEVAVPEILHRD
                     RTAGGYAGVYEDPSGRRKLSVSSVDGGWALEQGSERISLEPTGEADRFIVRHPDFAHF
                     PLVFARKNEKDPKSAVVEVSWGGEWFAGSAYEGPRKFSYPALWDSYVGHYRNESPWIG
                     SMRVVIRKGRLWLDGVIPLEAAGDRFYLRDEETSPEWVQFGPSINSRCMLLKFSGVDM
                     WRVAAT"
     gene            complement(12531..14414)
                     /locus_tag="VFB37_08180"
     CDS             complement(12531..14414)
                     /locus_tag="VFB37_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22471.1"
                     /translation="MRPLERALLISSCLLILLSACDAGISESSSGVSGTQSSDAGGSS
                     GSGGGGSTTGGLGGSPAGPSGSKDTVTATASVGGTLSVAVGSSETISVIFNSSDGRPI
                     RGLAISDTTLPAGWSGIDDYSCTQVGSGSSCIVNLTYSPTAVATGTLDLNYIYIDNSN
                     DAQTPGATVVIPYAATSNNNVIASAAPTGEISASLNAGAATVVINFTTDDGNAATALS
                     VISDLTALPTGWTAGSASFSCAIVSTGSGCQLLLNYTPNSVANGTLVLAYTYIDDGGA
                     SRSSSLNIPYSTTTAGNVLATWAPGGQINAIQKTGQQGVTITFTTDDARPASSLQLQT
                     SLKSLPAGWSSKSSALSCGSVSTGNGCQLTLTYAPVTPGRGTLVLLYDYIDATGTYSV
                     GSVNVPYASTTNDNVVGTAAPSGQINAIVGMGSQPVSVTFTTDDARPATALQLTSDLT
                     ALPGGWSSLASSFGCSGLASGSGCQLALMYTPAAAGTGTLALTYSYINNAGQAKTGAV
                     DIPYRSTTDDNVVATPSTNPLSLPAGSSAPITVTFTTDDGNPATALAITSGLSALPAG
                     WTSTSGAFTCATLSIGTSCQLALTYAPTMPDMGTLSLGFSYTNNAGMLKSGTVSISYT
                     ATP"
     gene            14622..16172
                     /locus_tag="VFB37_08185"
     CDS             14622..16172
                     /locus_tag="VFB37_08185"
                     /inference="COORDINATES: protein motif:HMM:NF005702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO22472.1"
                     /translation="MNINPSAPETDPAQWIERAAREHPLRPFLSTPSGRQISYAQLHE
                     QSGRFAAALARLGVASGDRVAVRVDKSAEAVVLYIACLRAGAVFVPINVASSPNEVEY
                     FLADAGPALVVVDPAESGALQAAGMRPGTDRIETLGAEGEGTLAQIAQQMGPNDSPST
                     TPDPRAPAAIIYTSGTTGRSKGAILTRGNLASNARALADTWCFTQDDVLLHALPLFHV
                     HGLFVAINTVLASVASIVLLPRFSCDLILANLAHATVYMGVPTHYTRLLQERGLRRET
                     TAHMRLFISGSAPLLPETHHAFLQRTGHRILERYGLTETLMNASNPYEGMRVPGSVGL
                     PLPGVLVRVRGANGEMVAAGADTIGTLEVKGPNVCAGYWRDEQKTRSEFTADGWFKTG
                     DLARIDRDGYVYIVGRAKDLVISGGYNVYPKEVESELDSVRGVLESAVFGVPHPDLGE
                     GVTAAVVLERGAALSEAQLIDLVRPRLARYKVPKRIVLVEELPRNAMGKIQKSVLRAR
                     YAPLYGGA"
     gene            complement(16169..18229)
                     /locus_tag="VFB37_08190"
     CDS             complement(16169..18229)
                     /locus_tag="VFB37_08190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZO22473.1"
                     /translation="MAPPPYRADIDGLRAVAVLSVIGFHAFPRSVPGGFVGVDVFFVI
                     SGFLISGLILQALSANRFSYSAFYARRIRRIFPALLTVLTAVLVGGVFMLFPDELQEL
                     SRQALASVGFAANFYFWSQSGYFDSSAPLKPLLHLWSLGIEEQFYIVWPMLLVCLYRR
                     ARQLKTIVGVLALASFLWNVASVATSAQSAFFLPLARFWELLLGALLAIQATETLSPG
                     AIVPYELAHVAGGPRAAASVTLTGRSDARRPSVVKSDLLCIAGLLLIGATVLRLTEHQ
                     QFPGWWALLPALGAALVIAGGRSRVGRLLLANRPMTLIGLISYPLYLWHWVLLSAVQY
                     VSYQNASHALRLAAIAASFVLAWLTYLLIETPIRSGRRLGTMVPALAASAALVAAVSI
                     AIDASDGVAMRYPPPVRKLATYRYDAHRAYREDLCQLNGDQAFTELDPRCVDPADAAT
                     RLVVLWGDSHASSLYQGLRAAQVRRGDFRIAQFAASGCPPLLGVRVGPRRDCKEFSAE
                     AFQRIQLLQPDTVVMGGAWYSYATRAHDGADELRALRETIDKLIGAGVRRVVVFGDLP
                     VWKIHQPNVAVKLWEQSHSLPGRTYDSFDRGSAAFDSQVSAVVLQTSAVFVSPVESLC
                     NEEGCLLNADPAHPVPVTWDFAHLTDAGSRLLIDLNAAKIFGTDNEGWRAGDAESAPT
                     AGVR"
     gene            complement(18761..20269)
                     /locus_tag="VFB37_08195"
     CDS             complement(18761..20269)
                     /locus_tag="VFB37_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO22474.1"
                     /translation="MSAVIKESPASTRVGQFLGTPKKLLIDGHWVAAASGKTFEVKDP
                     ATAATIARVAEGDKADVDLAVQAARRAFESGPWPALTPSERGKLVWRIGELIAKHADE
                     LAELESLDNGKPMAVARIADVPLAADIFQYMAGWSTKIEGKTIPLSVLYTPGAQYHCY
                     TRPEPIGVVGQIIPWNFPLLMAAWKLAPALATGCTVVLKVAEETPLSALRLGELLQEA
                     GLPDGVVNIITGYGETAGAALAAHPGVDKVAFTGSTEVGRLIVQAAARDLKHVSLELG
                     GKSPNIILRDADLETAIPGAANAIFFNHGQCCCAGSRLYVHESIFERVAEGIAEQARA
                     IKLGPGRDPDSQMGPMVSEIQQRRVCGYLESGTKDGARAITGGKAREGSGYFVEPTVF
                     VDTRPDMKIVQEEIFGPVVTVSPFADVNDDLVRDANDTIYGLAAGIWTRDVSKAHSLA
                     ARLKTGTVWINCYNVFDAALPFGGYKQSGWGREMGEAVLDNYLNTKAVTIGL"
     gene            20549..22660
                     /locus_tag="VFB37_08200"
     CDS             20549..22660
                     /locus_tag="VFB37_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO22475.1"
                     /translation="MADVERDILGADRKDPEPAAADPKPALPGGAPLPAGDIARASPF
                     SDAEALRGHLAAIVASSDDAIISKTLNGVILSWNKGAERMFGYTSEEAVGKPITILIP
                     AEQLDEEPVILAKVRRGERIDHYETVRVRKDGGRLYVSLTVSPVRDGSGRIVGASKIA
                     RDITHQKSAESQVRASEQRFRLMADSAPVLIWMSDASRARTWCNKAWLDFTGGTLEHE
                     LGFGWSEHVHPDDLAGYLRTYVESFDVCKPFRTEFRLRRHDGETRWMIDTAVPLREGP
                     VGTFSGYIGSCIDITEYRQSQLDREDLLRAERAARTEAERLSRVKDEFLATLSHELRT
                     PLNAILGWSTLLRRVGRESPDFARGLETIERNARAQSQIIGDLLDMSRIISGKVQLDI
                     QTVDPHEVIEAALESVRPSAEAKGLRVRMRVQGRVELIRADPSRLQQVLWNLLTNAVK
                     FTPPRGLIDVTVKRVNGHVEIVVEDTGIGVKPEFLDLIFDRFRQADASTTRRHGGLGL
                     GLSIVKQLVELHGGSVSVKSPGLDQGTTFTVMLPMSATRADDDTVRQPRPTFADVDFA
                     AVDLPSLSGVKVLVVDDEPDSRVLVSRLVEDRGALAIPAASAEEALHRIVKEPVDIVV
                     SDIGMPEVDGYQLMRQIRGLERETLRGVMAIALTAYARPADRQRALLAGYQMHLCKPV
                     DPRELVVGIASLLNIPKQMAH"
     gene            complement(22715..23329)
                     /locus_tag="VFB37_08205"
     CDS             complement(22715..23329)
                     /locus_tag="VFB37_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22476.1"
                     /translation="MNNSNRLAAGAWLAAGVATLLMFACSSQKAPAEQAVADIDTSLG
                     AVHDTAAKYSPEMLQNVEGQVATLKGNLAKGDYKEVLAAAPAVNTAITSLKQDADTKQ
                     AAADAALAQTKQQWRTLSTDVPKMVDAIKAQVDTLSKSHRLPKGVTKASLESAKSGVA
                     ALDSQWTDATNAVSNEDYAGAVAKGQAVKDKATDMMKSLDMKTS"
     gene            23749..>24578
                     /locus_tag="VFB37_08210"
     CDS             23749..>24578
                     /locus_tag="VFB37_08210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO22477.1"
                     /translation="MSWHAQTLAAVTDYDDAPLRPFHLRVAIASTGGAFADGFGLGII
                     GIALSLAAPELGLGALWQGLLGGASLAGLFAGALLTGPAADRFGRRPFFAYNMLIAAV
                     ICALQYTARSGQELLLLRIAIGYLLGTDYVVSKALLSEFAPLRLRGRILGVLSIAWAG
                     GYACAYASGFALTVVGHEAWRWMLLASALPCLLITPLRLTMPESPVWLVKHGQPERAA
                     AVVHAKLGENIRPPRGTPARTSQAGRWRQLFGPRWRLRTLVACTFFTCQVIPYFAVGT
                     "
BASE COUNT         4311 a         7818 c         7950 g         4499 t
ORIGIN      
        1 atacagcagc gcctcgggtg ctctggcaat cacctggccc gatctcgcac ggagcgttcc
       61 ggagagaaga acccgcagct tgaagtagtc ggtgccctcc acgcgcaccc aggtgtcgct
      121 gcggtacgtg gcgtcggtta ccgacagcat gagagcggtc cagggcttgt aaaagtccca
      181 gtatccgtct ccctcgccga cgtggagata atggcggacg ctcgctgcag tccagcgacc
      241 cggcgggacg acatctaccg cggaggcggt cggtgatgca cccagcattt cacccaggat
      301 ccgctcggca tcgtcctgga gcatgccgac gatgggttgt cgggcaacca gaacgtcctg
      361 gggagcactc tcctgcaggt ccctgcggcg gccccggtcc tgcgtgccta ctgttccgac
      421 gaccattcga tcatcactcc ttcgtcgggg agatgtacga aaaatccgcc ctcgacggtc
      481 tcccaaacgc gatggccggc gcgctcagcg aactcgcgtg ctcgattgag attgtccgag
      541 gccaggccaa acccggcgag caacggctcg cggcgcgtcc cgaagcgcgg ggcttgtcgg
      601 aatcgctgct cgaaggaagc tgccgagagc aggctgatgc gggactcgcc gagccgcaga
      661 acagctccgc cggcgctccc cgtggccggc cggcccagca cccgctccaa gctgctcctc
      721 gtcgcgccgc tctcggcgag cacgatgagc tcgcgtaacc tgcgcgcccc gttggggtgg
      781 cgggcaacac ccggatgcca cacgaactgg ggggtcacgt gctcgcaaca gaacagcaga
      841 aagggtagct cgggcgttcg ctcgagcatg gccaggcgga agcgcgccac ctcgtcccga
      901 ccctctatct ccacccccct ggcaagatcc actggcgtcg agggctgcaa tccagccgcg
      961 ctccagagtt ggaacagggc ctgagccgag ccgcgcaggg cggttcccca caggcccgga
     1021 tcggccatca tgtcgctata ggcagctgct gccgaaggtt gcccggacga tccgaggagc
     1081 tcgatataag tgcgatccag catgatcagg tgattggccg tcccgccggt gcgatgaaaa
     1141 ccgcgcggcg tcgtggcaaa tccgaggctc tgccagatgc gctcggcgcg gtcgaggtcc
     1201 cgcaccatga caatgaggtg gtcgatgccc tgaacgatat cttcgtttgt actcaacttc
     1261 gtctcactgg cccccggtca accacgccgt aacgggcaac agtgtgccgc cctctctccg
     1321 ggacgacggg gtagtgcctc ccaaggccgt ccgatcgcag tagtcatgct tcggatcgga
     1381 tggctcacgc ccggcagtta ggatttaatc catctcgggc gaagaggcaa caaccgtccg
     1441 tacatgagta tatgaccgga tctcgagagg gccatgggac gggcagtccg ccgtcctccg
     1501 agcagatcgc ggcgctgctc gcagagagcc cctttcacca ggtgctggga ttgcggctgg
     1561 agtcggtcga tcccggcgcg cacagcatag ccatccgctg cgatttcggg ccacggctgg
     1621 agcgctctcc cggaaccggc cagtaccacg gtggcgtcat cgccagcatc attgatattg
     1681 caggcgattt tgcgctgatc gccgtgctcg gacacggggt gcccacgatc aattttcggg
     1741 ttgattacct gaggccggcc cgcaggggag gcctcatcgc gcgggcgcga gtgcgcaagt
     1801 cgggacgcac ggtaggcgtc gtggatatcg acatcgaaga cgacgacggc cgactcattg
     1861 cgatcggtcg cggctgctac ggtacccagg cgggctgaga tcgaggcaga cgttcaagga
     1921 caaaaccatg cgagcggtgc tgacggtctc acacggcggt cgagaggccc ttcaatatcg
     1981 gaatgacgtg ccgaatccgc aaccggacgc agcggaggcg ctcgtgtgtg tcgcggcgac
     2041 ctcggtcaac tatcacgaca tcttcactcg ccgtggtatg cccggtgtcc gcatcagtct
     2101 gcccgtcatt gccggatcgg atatcgccgg gaccgtgctt gcggcaggcc cgcgtgccga
     2161 tgcggcgtgg gtgggcaagc gcgtgctcat cgatcccgtg ctccggcaga gcgatcgcat
     2221 cgggatgctg ggggaaacac ttcccggagg ccgtgcggag ctgatcgccg tggcgcagtc
     2281 gatgctggtc gagctgccgg aggaagtgag tttcgaacag gccgcatcct tgccgctggc
     2341 ctacggcact gcacatcgca tgatggtgac ccgcggagaa gtgaaagcgg gggagcgcgt
     2401 gctggtgctc ggcgccagcg gtggggtcgg agtcgcctgc gtccagatcg cgaagctgct
     2461 cggggcggag gtcatcgcct gtgccagcag cgaggccaag ttgcagcgcc ttcgggaaat
     2521 aggcgccgac cacgtgatca actaccgtga gcagccgttt caggaggcca tcaagtcgct
     2581 gtgcggcaaa ccgagagtga gcggcgcggg aggggtggat gtggccgtca atttcaccgg
     2641 cggcgatacc ctcctgccca cacagaaatg cgtcaagttg ggagggcgca ttctctgctg
     2701 cggcgctact gcgggcttca acctccagct cgatgcgcgt tactggtgga cgttcgagca
     2761 gaccctgatc ggttcagacg gctggatgaa ggaggacctc gagaggctcc tggcgctggt
     2821 cgccagtggt cggctgcggc ccgtcattga ccgcatactg ccgctggagc agacggctga
     2881 gggtgagcgc ctgctggagg accgcgaggt tttcggcaaa gtggtcctga aaccctgaag
     2941 cgaaccgtat catgagcgag ctgctcaatc tcggcgacat cctgcggctg ccggcgctcg
     3001 gcgaccggct cgcctatctc gacctgcgcc ggccggatca gcctctgccg ctcaccgccg
     3061 aggagctcga tgagcgcatc cgcgccatcg gccgcgggct cgtgcgacgc ggcattcgat
     3121 tgggcgagcg cgtcggaatt cttgccgaga accgtgttga gttccttgca acatatctcg
     3181 cgatcatgcg catgggggcg gtcgcggtgc cggtcaattt caaactggcc ggagcgacca
     3241 tcgcacatat catgcgtgac agcgccgtca aactcgtgtt cgtggacgcg cagcgcacgc
     3301 ggcaggttcc ccccgggatg ccgatcgtct ccttcgacgc cgccgccggt aaggagttcg
     3361 aggactttct cgatgcgggc ccgttcgaga cgttcgttcc cggggagcag gacctcgcag
     3421 agattctgta cacctctggc tcgaccggga tgcccaaagg cgtgccactg acgcaccgtg
     3481 gacaggtgtg ggcattggcc aattactgtg cggagctgag tgagggtgag ggtacacccg
     3541 aggaccgaac gctggtcgtt gcgcctctgt accacatgaa cggcctgttc tttagcaccg
     3601 tggcactggc taatcgcatg gcagtccttt cactgccgcg cttcgaagtg cgcacgtacc
     3661 tgaagactgt ggccgagcag cgttgcaccg tgctgtcggg aatccctacg atgttcgcca
     3721 tgatggtgcg cgaaaccgat ctcatcgaga ctctggatct gagctccgtg aaggaagtgg
     3781 ccatcggctc cgcgccgctc accgatgctc tgctcggcca ggttcaggcg ctatttccca
     3841 atgccgaggt ctccaacggc ttcggcacga ccgaggcggg acctgccgtg ttcggtgcgc
     3901 accccgacgg gctgccgacg ccgccgctct cgctcggcta cccgctggcg agcatcggct
     3961 ggcgcctggt ggatgggtct tcgagcgagg agggagtgct ggccttgcgc acgcctgcgc
     4021 tcatgggcgg ttatgtgaat ttaccgcatg tgacagccga gaagatgcgc gatggctggt
     4081 atctcacggg cgacatcatg cgtcgcgacg caggcgggtt cttctacttc gtgggacgcg
     4141 cggatgacat gtttgtctgc ggcggagaga acatctatcc cggtgaagtc gaaaagcttc
     4201 tcgagcgcca ccccggagtg gcccaggcga tcgtggtgcc cgcaccggat gacatcaagg
     4261 ggcagatccc cgttgccttc atcgtgcctc ggcccagcgt ggcaccgagc gctgatgaga
     4321 tcaaacaata tgcgctcgag aacggagcgg cgtacgcgca tccgcgtttc gtggaatttc
     4381 gggatctgct gccggtttca gggacgcaca agatcgatcg ggccgcgctg gtgaaggaag
     4441 cggcgcagct ctcccgcgct gcgggcagga gccggatttc cctggaaaag agctcgtcga
     4501 cggtcgccgc tgcagacccg cactcgtcgc ggaaataatc tcaaggcagg tccaccgatc
     4561 cgatgcaggt gccctcaaga gtgcagttat ccgcagatga tccgcgtgca atcgcgaatg
     4621 tcccggccgg cccggggcgc gagcgggcat ggctgggata cccacgcggc gcctggctcg
     4681 tcatcagtgt cgagttctgg gagcgcttca gcttctacgg gatgctggcg atcctggcgc
     4741 ttttcctcac cgatgacgtc gctcacggag gcttcggctg gtcgtccgcg tccgctctgg
     4801 ccctcgtggg tgcctattcg ggggccatgt acgcattccc cgcgttcggc ggatatttag
     4861 ccgatcgcgt gttgggacgc cggcgtgccg ttgcccttgg cgcgacttgc atgctcatcg
     4921 ggcaatcgct caccgcctcc cccgcctatc tgcccaagct gctgggcttg tggcaccgtc
     4981 agccattgct gcccgccttg cgctcgatcg gcgtgccgct cggtcaattg cggcaaagtg
     5041 ggcagttgtc cgccgcgctc cagatgcacg gcgcggcgct ggacccagtc catggagcca
     5101 cctggctcgc tcaggcctac accgcgagtg cgatgggctt ctacgccgcg ctgttctttc
     5161 tgatcctggg caacgcggtg atgaaatcga ctctggtcgt actctgtggc gagacgttca
     5221 ggccggacga ccctcgccgc gaaggtgcct ttgcctacta ctatctcggc atttccgtcg
     5281 gagcgatgct gtccggattc gtggtgggca tcgcctccga acgctacggc tgggcttcgg
     5341 gattcgccgt ggcggccatc ggcatggccg tagcgctgag cctgtacctg ctgctgagca
     5401 agcgttggct tggaaacatc ggcgagcaca gcgcggcgac gtccgattcg aataccgtgt
     5461 ccgctacgcc gcccgccgcg cccgctgccg ccgagacgtg gcggcgcatc atcctggtgc
     5521 tggtgctcgc ggcgttgatg tgcatcttca gcacgggctg gtttcagatg tatggcagct
     5581 ggtcattgtt catcgagcac tcggtggacc gcgcggtggg tcccctgacc gtacccgtgg
     5641 catggttctc atcgatcaac gcggtcgtcg tgatttcgat cgctcccatc ctggcgagcc
     5701 tgtgggttcg gcttgcggcg agcaaccggt cggtcaatat catccagaaa ttcttcttcg
     5761 ccattgtcat ggcgatgttg gggaatggcc tgatgtactg ggccggagcc ctggccggcc
     5821 ggggccagct ggctcccatc tggatgccct tgctcggtgt ctcgatgttg ggttgcggcg
     5881 agatcgtggc gtggaccgcc acctacggct ttgtcactcg ggccgcaccc gcggggttcg
     5941 catcggtcac catgggagcc tggtatctgc tgacgctggg tttgggagga tacctttccg
     6001 gggtcaccgg gaattgggtg gacacggttg gcttcaccgc gacatttggg atcattgcaa
     6061 tagtgatggg cgtgggcgcg gcggccgctc tggtcatgcg tgcacctttg ctgcggctcg
     6121 ccgcgcgcgc acgagtcgaa ctgtgatgag agtcctgtga ggatgcatga atgacaaata
     6181 accacggcat ctcacgccgg cgcttccttg cccaaagctc ggcggccacc gcatcggccg
     6241 ccctgtctct gggatggcgc cgcgcgcact cgcggcagaa ggacaacgct gcgcagctga
     6301 cctcactgag cgccgtgaag gcagtggcgg cactgcgcaa cggggatatc accgccgagg
     6361 cctatgccgg cgcactgttg ctccaagctc agtcgggcgc gctgctcaat gccttcatca
     6421 ccctgcggcc tgaagccgtg ctcgaggcgg ctcgcgactg cgatcgccgc aggcgcgcgg
     6481 gtcagccgct cggtcccctg cacggcctgc ccatcccgat caaggacagt gtcaacacgc
     6541 gggacctgcc caccaccgcc ggaacaccgg cactgcggca tttccagccg gccgccgatg
     6601 cgccgacggt ggccatcctg cgccgcgccg gtgccatcgt gctcggcaag acgaatctgc
     6661 acgagctctc atacggctat accagcaaca accatgctta cggtgcagtt cgcaacccct
     6721 acgatcctac acggatcccc ggcggcagca gcggcggaac cgcggcggca atcgcctacc
     6781 acatggcacc actcggaatc gccgaagata cggaaggctc catccgcgtg ccggcagccc
     6841 tgtgcgggct ggctggattt cggcccacca ccggccgata ttcgacggcc gcctgcgccc
     6901 cgatcactcc gctgttcgat cagttgggcc cgcacgcgcg ctcggtggaa gatctgttgc
     6961 tgtttgacgg tgtggtcacc ggcgatgtgc aacccccagc cagcgcctct ctcaaggggg
     7021 tgcgactcgg actgatccga tcattttact ggtcgggcct ggatcctgag gtcgaacgca
     7081 tcaccgatgc ggcgctcaaa cggctgactg acgcaggcat cgagctcgtc gaggcggagt
     7141 tgccggagct gccgcatttg atcgccttga cgaccgatcc gatccagaac cacgacgtca
     7201 aaccttccct gacgcgctac ctggccgaat atcatacggg cgtcactttc gagtccctgc
     7261 tcgcgcaggc gagtccggat gtgcgggaga tgatcgagcc ggccatcaca ccgggtggtc
     7321 ctgactacgt gcccgaggcg cagtaccgag cggccgtcaa cgtacacttg ccggcgctgc
     7381 gcaggttgtt caaggactgc ttcgcgcgcg cgcagatctc ggcaattgtc ctccccacga
     7441 cgattctgcc cgctcccaag ctcggcgagg aaaacatgga agtggaggtc cgcggccggc
     7501 ggatgccgct tgatgtcgcc atcgcacgca acattgcgcc gggcagcaca gcgggcctgc
     7561 caggtctcgt gctgcccgca ggattgaccc cgaatggcct gcccgttgcg ctgcagttcg
     7621 acggtccctc gggatccgat cgggctttgc tctcgctcgg actcggcttg gagtccgccc
     7681 tcggcaggct cgccccgcct ccgaagttgc caggttagcg gcgccgattc caggtgtgcg
     7741 agctcagctc gaggacagca cgaactggtt gccatccgga tccttgaagg tcgcgaacgt
     7801 tccccagggc tgccgctgag gttcgccagt gaactcaacg ccccgctggc gcagttgccg
     7861 gtacgtcgcc tcgacatcat cgcaggcaag ggagccgttg aagaaggttc cgatacggct
     7921 ttcgtgcccc tctggagtga aaagcaccac ccgcgtctgc gagtgcgcga cgcgcagctc
     7981 gatccagcgc tggtgggcat cgaagggctg atcggtggag acctcgaacc ccagctgctc
     8041 ggtgtagaac ttgagggcac gatcctggtc ccgggtggga atgccgacga acttcacgtg
     8101 agtgatcatc agagcgtcct cgaatgagcc tgtgcatatc ctagcgcgac cgccgcggcc
     8161 ggattatctc tatctgctat agtacttaat gatatgcagc gcctgagcat cgacgcttac
     8221 gtgaccgatg tgctgatgcc ggacctcgtg ggtcacgatc gccggccggc cgccttcgtg
     8281 gtgtatctgt ttctcctgcg gcacagcggg cagcgctcga gcgaccgggt gagcgcgagt
     8341 ctgcagacca tcgccacgcg cacgggcttg tcgaaatcga gcgtgcagag ctcgatccgg
     8401 catctgaagc gccgccgcct gctggacccg gatctgaagg ctaccgtgac cgagcccgtg
     8461 cggcgggtgc tgcggccctg gctacggtaa aggggctcgg ccgcgcgcgc aaacgcaaca
     8521 gcaagaatcg gatcgactcg cgaatgcatc cgggccaggc tgtcggagcg cggtgcgatg
     8581 gccttggcgc cgcgtaggcc agtgccgcgt ggccggcaga atcgaatatc agaccatgaa
     8641 tcgcttcccg atcttcatcc tgcagacgct cgtgattctg acctcatcga tgtggccggc
     8701 aacgctcctg gcggccccgc ctgtcgcatt gtatgaggtc accacggaga tcgggatgcc
     8761 gcatctggag gaaaatctcc gctacacgat cacgcacgag cggcgctgcc tgtcccgcga
     8821 cggactgcat cggttgtttc ccattttgca gcacccggct ctcgcgggct gctatctggg
     8881 tgccccagcg atctccagtg cccacgaagt gacctatccg ctgctgtgcg acggcaatca
     8941 tggaacaacc ggcgtggcac gctgggaagc cggataccgg cggctcacgg gcacactggc
     9001 tgtcaaactc ggtggcaaga acatgacctt tttccagcgc gtcactgcca ttgcgctcgg
     9061 cggctgcaaa gcgagcactc cctgagcgca cgagccggct tgacatgccg gccgcctacc
     9121 gtgcgatcaa gtcgatgaag cggcgatgat gaccttcagt ttcccggcgt cgagcgagct
     9181 ctggacgaca tgcatggcgt gcgcggtatc ccgatagatc agggccggag ttccgcgcgc
     9241 gcccagatcg tgcatcaggg cttcgttcga cgcgatggcg gcgagcgtcg cgggtcgagg
     9301 cgtacgcaac ggcggaatac caccaccgag gtcatcgggc aggctggccc agcgcctttc
     9361 gttgcgatcc agggcctgtg aggggttctt ggcctcgaga atggcggcgg ccttgcccgg
     9421 actgttccgc gcgagaatgc ccaccatgat gtagcgcacc tgcagattct cggcatacag
     9481 cggctgaagc gataaccaca gatcgtggca gaaggggcag ttggcatcgg ttatcacgta
     9541 caggatgtgc tgcgggtgag ttgagccttg cgccgcccag gatgcccgct gaagccgcaa
     9601 ccagacatcg ggtgtcccgg ggtcgggact ccagggcggc ggggcggcag cgccggaccc
     9661 cgcatagaac aggcaaatcg cagccgccgc gagcagcaca acgcgccggg acgcggtggc
     9721 aaggtgacgg atgggggtcc ctatagcaag ggcctgacgc attgatccgg gtatgttcac
     9781 tcagtctcct cgcacaccaa cattcgttcg gacggattca ccacttgcgg cccgcagggt
     9841 ccgtggcaca aggcgcgcat cggcaaactc tgcggcgcag gccgcgaatc ctagcgttgg
     9901 accacgccat ttcagctcat cgacagttgc gaacagcctg gcctcgcttt tgagcgtggc
     9961 cagctgtttg aagcgcaagg ccagttcgcg tcgctcgccc agaatttccg gcggaaatgc
    10021 ctcgattgcc ccgtagcgct cgatgagccg ggcggcggtg acggcaccga tcccactcaa
    10081 gcccggatag ccatccgcgg tatcccccac caaggccagg aaatcgggaa tgtgccgcgg
    10141 ctcgacgcca aatttctggc gaaccgcctg cgcgttgcgg atcacacggg tcttgcgatc
    10201 gacctgtacg atgcgatcct ccacgacaca ctgggcgagg tccttgtccg gagtccagat
    10261 acatactttc tgcacgcacg catcggcccc ggcgagccgt gcggcagagg cgagcgcgtc
    10321 atccgcctcc agctcgatca tcggccagac ggtgacaccc atcgctgcga gcccgcgctc
    10381 gagtggatga aactgcgcaa gcagcgccgg ctcgattccc tcccccgtct tgtagcccgc
    10441 ccacaggtca ttgcgaaacg actcgatgac gtgatcggtc gcgacgccca gatgcgtagc
    10501 cccttcctcg atcatctgca agatcgtgtg cagcaccccg ttgaccgcac cgaatggccg
    10561 gtcttgtccc ttgttgaagc ggcgcaggcc gtagaaatgg cggaacagct cgtacgtgcc
    10621 atcaatcagg tgaacgatca atcgcgcctc cagggtggcg cacagtctac ggctcccgca
    10681 cggtttgcgg ccacacgagc gcgccagtag tggtgaccat ggtgcaccgc gacgcttgcg
    10741 tctatattgt cgcgttctta cgccgggcca cgcagaaaga ccgttggggg acatcaatgc
    10801 ttttcaatcg cagacagttg ctggcgagcg gtctcaccgc tacggccgta gcggccaccg
    10861 gccgtttcac ggtggccggc acgcctcccg atcacggtgg gtccgatccg caagtgaacg
    10921 cgcggctgga tcagttcatc gagcaataca tgcgcgagtt gcgatccccg ggcatgacac
    10981 tcgttctggc ggaccgggat ggcatccaga gggtggccgc gtatggtttc ggcgacctcg
    11041 agcgcagtcg cccggtgcgc accgatgagc tgttcgagat cggctcgatc agcaagtcgt
    11101 tcgtcgccct gtgtctgttg cagctgcacg atgaaggcaa gctcgatctg cacaagccgg
    11161 tcgtggacta ccttccctgg ttccgggtcg aatccagatt cgcacccatc accacccatc
    11221 atctgctcac ccacggctcg ggcctgcccg gaaatgcgga cgtctttctc tccgacccgg
    11281 cggatcgtca tcgcgcggct tatgcacccg gggagcattt ccattacaac aacatggcct
    11341 ttaccctgct cggtcatctc gtctggacgc tcgatggacg cgagctgccg caagcgatcc
    11401 gcaagcgcat tctcgagccg ctcggcatga cccagacaga gcctgcgatc acgctggacg
    11461 tgcgcgagcg cacggtcaag agctactgcg cgctccagaa cgaccggccg gattcgccga
    11521 gcatgccgtt gtccgaggcg cctggactca tcgagaccag cggggcgggc tgcgtcgcct
    11581 cgaccgcacg tgacatggga tcttacatcc gcatgctggc caatcatggc gcgggtccga
    11641 atggcaggct cgtctcgaag gagagctttg cgctcttctg tcagccgcac atcgcggcgg
    11701 atgagttcgg gcctaccgcg cattatggtt atggcatcgc cgtcgatcgg ctggagggca
    11761 acactgtcgt acgacacacg ggcggaatgg tttcattcat gtccgccatc atcgtcgacc
    11821 tcgatcaggg ggtaggcgcc ttcgcatcga tcaatgcgat gcagaactat cgtcccacac
    11881 ccgtggtgca gttcgcagcg ctgctcatgc gcgcgacgaa gcttcgcaag aatgtcgcag
    11941 aggtcgccgt gcccgagatt ctgcaccggg atcgcaccgc cggcggctac gcgggcgtgt
    12001 atgaggaccc ttccggtcgc cgcaagctga gcgtcagcag cgtcgatggc ggctgggcgc
    12061 tggaacaggg cagcgagcgc atttccctgg agccgaccgg tgaagcagat cgattcatcg
    12121 tccgccatcc cgacttcgca cacttcccgc tggtcttcgc ccgcaagaac gagaaggatc
    12181 cgaagagcgc ggtggtggaa gtgtcgtggg gcggcgagtg gtttgccggc agcgcatatg
    12241 agggcccgcg caaattcagt tatccggccc tctgggacag ctatgtgggc cactatcgaa
    12301 acgagagccc ctggatcggc agcatgcgcg tagtcatacg caaggggcgc ctgtggctcg
    12361 acggtgtcat tccactggaa gctgcgggtg accggttcta tctgcgcgac gaggagacca
    12421 gtcccgagtg ggtgcagttc gggccgagca tcaactcccg ctgcatgctg ctgaagttct
    12481 cgggcgtgga tatgtggcgg gtggcggcca cctagcttga agcgagcccg ttacggcgtc
    12541 gctgtgtagc ttatcgagac cgtcccgctc ttcagcattc cggcgttgtt cgtgtagctg
    12601 aagcccaaac tcagggtacc catgtcgggc atcgtcggag cgtaggttag agcaagctgg
    12661 caggaagtgc cgatgctcag cgtcgcacag gtgaacgcgc ccgacgtact ggtccaccct
    12721 gccggcaggg cgctcaatcc cgatgtgatg gcgagggccg tcgccggatt gccgtcatcg
    12781 gtggtgaagg tcactgtaat gggagcggag ctcccggcgg gcaacgagag cggattcgtg
    12841 ctcggcgttg ccacgacgtt gtcatcggtg gtcgaccggt aaggaatgtc caccgcgccg
    12901 gtctttgcct gcccggcatt gttgatgtac gaatacgtca aggcgagtgt gccggtgccg
    12961 gcagcggcgg gcgtatacat cagtgccagc tgacacccgc tgccactggc caacccgctg
    13021 catccgaatg agctcgccag gctgctccag ccgccaggta atgcagtgag atcgctcgtg
    13081 agctgtaacg cagtggcggg gcgggcgtcg tcggtcgtga acgtcaccga aacgggctgg
    13141 cttcccatgc ctacgatcgc gttgatctgt ccggaaggag ccgctgtgcc gaccacattg
    13201 tcgttggtcg tcgacgcata cggtacgttc accgaaccca cgctataggt gcctgtggcg
    13261 tcgatgtaat cgtaaagcag aaccagcgtg ccgcggccag gggtgacggg ggcgtacgtg
    13321 agcgtcaact gacagccgtt tccggtactg acgctgccac agctcagcgc ggacgatttg
    13381 ctgctccacc ccgccgggag cgacttcagg ctcgtctgca actgcagact gctcgccggc
    13441 ctggcatcgt cggtcgtgaa ggtgatggtg actccctgct gaccggtttt ctgaatggcg
    13501 ttgatctgac cgccgggggc ccacgtagcg agtacgttgc cggctgtggt cgtggagtac
    13561 gggatgttga ggctactgct gcggctcgct ccgccatcat cgatgtacgt gtaggccaga
    13621 acgagcgtcc cgttggcaac cgagtttggc gtgtagttga gcagcagctg acagccgctt
    13681 cccgtactga cgatggcgca ggaaaaggac gcgctgcctg ccgtccaacc ggtcggcaaa
    13741 gccgtcaaat cactgatgac agagagcgcc gtggcggcat tgccgtcatc ggtcgtgaaa
    13801 ttgatgacga cggtggctgc gcctgcattc agactggcgc tgatctcgcc ggtgggggca
    13861 gctgatgcga tgacattgtt gttggacgtc gcggcatatg gaatgaccac cgtcgctccg
    13921 ggagtctggg cgtcattcga gttatcgatg tagatatagt tgaggtcgag cgtgccggtc
    13981 gcgaccgcgg tcgggctgta ggtcagattg acgatgcagc tgctgccgga gccgacctgg
    14041 gtgcagctat aatcgtcgat tccgctccag cccgccggca atgtggtatc cgagatcgcc
    14101 aggccacgga tcgggcggcc atcgctcgag ttgaagatca cgctgatggt ctcgctcgaa
    14161 ccgaccgcga cggacagcgt cccgccaacc gaggcggttg cagtaaccgt atccttgctg
    14221 ccgctgggac cggccggcga accgccgaga ccgccggtcg tcgagccccc gccgccggag
    14281 ccgctagagc cgccggcatc gctgctctgc gtgccagaga cgccggagct actctcggag
    14341 atgccggcgt cacaggctga gagcagaatc agcaggcagc tgctgatgag gagtgcacgc
    14401 tcgaggggtc gcatttccgt ggtcctcacg agcctggacg gtcggctccc gcggatgcaa
    14461 gcctaccgct cagccgctcg gacgcggccg gagagttggt taactgtgtc acgaaaaagg
    14521 cgccggccgg cccagtgagc ccgaccggct ggacataaca tccgagcctg tcgacgtgaa
    14581 cggactcagc tctccatggc atcatgccgc cacgttcgcc tatgaacata aatccatccg
    14641 cacccgaaac cgacccggct caatggattg agcgcgcggc gcgcgaacat cctctccggc
    14701 cgtttctcag tacaccctcg ggtcggcaga tcagctatgc gcagctgcac gagcagtccg
    14761 gccgcttcgc cgccgcgctc gctcggctgg gggtcgcatc gggcgatcgg gtcgcggtgc
    14821 gggtggacaa gtccgccgaa gcagtcgtgc tctacattgc ctgcctgcgc gccggcgccg
    14881 tcttcgtgcc gatcaatgtg gccagctcac cgaatgaggt ggagtatttt ctggccgacg
    14941 caggtcctgc cctggtagtg gtggatccag ccgagagcgg cgccctgcag gccgcgggca
    15001 tgcgccccgg caccgaccgg atcgagacgc tcggcgcaga aggcgagggg acgcttgcgc
    15061 aaatcgcgca gcagatgggc ccgaatgatt ccccctctac aacaccggat ccgcgcgcgc
    15121 ccgctgcaat catttacacc tcaggtacga cgggccgatc caaaggcgcc attctgactc
    15181 gtggcaatct ggcctccaat gcgagggcgc tggcggatac ctggtgtttc acgcaggacg
    15241 atgtgctgct gcatgccctg ccgttgttcc acgtacacgg cctgttcgtg gcgatcaaca
    15301 cagtgctggc ctctgttgca tcgatcgttc tcctgccgcg gttctcctgc gacctgattc
    15361 tggcgaattt ggcccacgcc acggtctaca tgggcgtgcc cacgcactac acgcgcttgc
    15421 ttcaggagcg cgggctgcgc cgggagacga ccgcacacat gcgtcttttc atatccggct
    15481 cagcgcctct gctccccgag actcaccatg cattccttca gcgcacgggc caccggatcc
    15541 tcgagcgtta cggtctcact gaaacactga tgaatgcctc gaatccttac gaaggcatgc
    15601 gcgtaccggg ctcggtggga ttgccgctac ccggagttct ggtgcgagtc aggggggcca
    15661 acggcgagat ggtggccgcg ggtgcggata cgatcgggac cctcgaggtc aagggtccga
    15721 atgtctgtgc cggctactgg cgggatgagc aaaagacgcg cagcgagttc acggccgacg
    15781 ggtggttcaa gaccggtgat ctcgcacgaa tcgatcgcga tggctacgtc tacatcgtcg
    15841 ggcgagccaa ggatctggtg atcagcggtg gatacaatgt gtatccgaag gaggtcgagt
    15901 ccgagctcga ctcggtgcga ggtgttctgg aaagtgccgt gttcggtgtg ccgcatccgg
    15961 atctcggcga aggcgtcacc gctgcggtag tgctggagcg cggcgccgcg ctgagcgagg
    16021 cacagctgat cgatctggtg cgcccacgac tggctcgcta caaggttccg aagcggatcg
    16081 tgctggtcga ggagctgccg agaaatgcga tgggcaagat acagaagagc gtgctgcgcg
    16141 cgcgatacgc ccccctatat gggggcgcct aacggactcc agccgtcggc gcggactccg
    16201 cgtcccccgc gcgccagcct tcattgtcag ttccgaagat cttggctgcg ttcaggtcga
    16261 tcagcagacg tgagccggca tccgtcaggt gcgcgaaatc ccaggtgaca ggcacaggat
    16321 gggcggggtc ggcattgagc aggcagcctt cttcgttgca cagtgattcc accggcgaga
    16381 caaataccgc ggaggtctgc aagaccaccg cagagacctg tgaatcaaac gcggccgacc
    16441 ctctgtcgaa ggagtcgtac gtgcgccctg gcaggctgtg cgattgctcc cagagcttca
    16501 cggccacgtt cggctgatgg atcttccaga cggggagatc cccgaacacc acaacgcgcc
    16561 tgactccggc gccgatgagc ttgtcgatcg tctctcgcag ggcgcgcagc tcgtctgcgc
    16621 cgtcatgcgc gcgcgtcgca tacgaatacc aggcgcctcc catcacgacc gtgtccggct
    16681 gcaggagctg aatgcgttga aaggcttctg cgctgaattc cttgcagtcg cgtcgaggcc
    16741 cgacgcgcac gccgagcaga ggcgggcaac cgctggcagc gaactgtgca atccggaagt
    16801 ccccgcgccg aacctgcgcc gctcgcaggc cctggtatag cgaagaggcg tgcgagtcac
    16861 cccaaaggac gaccagcctg gtggccgcgt ccgccggatc gacacatcgc ggatcgagct
    16921 ccgtgaaggc ttgatcacca ttgagctgac acaggtcctc acggtatgca cggtgcgcat
    16981 cgtatcgata cgtggccaac ttgcgcaccg gaggcggata acgcatggct acgccgtcac
    17041 tcgcatcaat cgcaatggac actgcggcga cgagcgccgc tgaggcggcg agtgccggaa
    17101 ccatcgttcc cagccgtctg ccggagcgga ttggtgtctc gatcagcagg taggtcagcc
    17161 atgcaagcac gaagcttgcg gcgatcgcag ccagacgtag tgcatgcgag gcattctggt
    17221 aagagacgta ctggaccgcg gacagcaaca cccagtgcca gagatatagc gggtagctga
    17281 tgaggccgat gagagtcatg ggccgatttg ccaggagaag acggcccacc cggcttcgtc
    17341 cgccggcaat caccagcgcg gcgccgagcg cgggcagtaa tgcccaccac cccggaaact
    17401 gctggtgttc ggtcagccgc agcacggttg cgccaatgag cagcaatcct gcgatgcaca
    17461 gcaggtcgct tttcacaacg ctcgggcggc gagcgtcact gcgccccgtg agcgtgacgg
    17521 atgccgccgc gcgcgggccg cccgcgacgt gtgccaactc gtacggcact atggcgccag
    17581 gcgagagagt ctccgtcgcc tgaatcgcga gcaacgcccc cagcagcagc tcccagaagc
    17641 gggccagcgg cagaaagaag gcactctgcg ccgaggtggc aacgctcgcc acgttccaca
    17701 gaaaggaggc gagcgcgaga acaccgacga tggtcttgag ctggcgcgcg cggcggtaaa
    17761 ggcacaccag cagcatcggc catacgatgt agaactgctc ttcgatgccc agggaccaca
    17821 ggtgcagcag cggcttgagc ggcgccgagc tgtcgaagta cccggattgg ctccagaaat
    17881 aaaaattggc ggcgaatccg acgctcgcga gggcctgcct gctcagctcc tggagctcgt
    17941 ccggaaacag catgaagacc cccccgacca gtacggccgt gaggactgtc aagagcgcgg
    18001 ggaagatgcg gcgaatccgg cgggcgtaga aggcgctgta cgagaagcgg ttggcgctca
    18061 gcgcctgcag gatcaggccg gagatcagaa atcccgagat gacgaagaag acatcgacgc
    18121 ccacgaatcc accgggaacg cttcgcggga aagcatgaaa gccgatgacg ctcaatacgg
    18181 ccactgctcg cagcccgtcg atatccgccc ggtacggtgg aggcgccatt gccgatggcg
    18241 ccgcggcggg gcgccctgag tggtccatcg ccaacgtcct gccgtgctca gaagtggtca
    18301 acaccgcctc cgtcccccaa catcggtcag attgatcgag caggcccgcg cgcccggact
    18361 gtatgggccg cgaatcggtg agctccaaac gcttccgtcg gccagtcacg cggtcgacgc
    18421 gagaatgcga acaccgttgc gatgaccggg ctgaccgggc attcctggct gatccagggg
    18481 gccgcccatc cgggcgaagt gcgcgcgttc tgcgccgcct ccgcggttta agtcatcacg
    18541 gcagcagctt acgacaagtt gctaccgcga tgctggaaga attttcgagc tgtctgcgtt
    18601 cgcagacggg catgcagccg cgggcgagcg caggctgtgg gctcaagcaa gccgagtggc
    18661 cactctggat tgcaaagcac gtccatcccg tacgtgcccg cgccaacacc aacacaggcg
    18721 aaggaccccg ccggagcggg gtcccgcgga ggctgtcatc tcacaatccg atggtgaccg
    18781 ccttcgtgtt gagatagttg tcgagcaccg cctcacccat ctcccttccc cagccggact
    18841 gcttgtatcc gccaaagggc agcgccgcgt caaatacgtt gtagcagttg atccacaccg
    18901 ttccggtctt aagtctcgcg gccagcgagt gggccttgct cacgtcacgc gtccagatgc
    18961 ccgccgccag gccgtagatg gtgtcgttgg catctcgaac gagatcatca ttcacatcgg
    19021 cgaacgggga cacggtgacg accgggccga agatctcttc ctgaacgatc ttcatatcgg
    19081 gtcgcgtatc gacgaagact gtcggctcga cgaagtatcc ggaaccctcg cgcgccttgc
    19141 cgccggtgat cgcgcgggcc ccgtcttttg ttcccgactc gaggtaaccg cagacgcgcc
    19201 tctgctggat ttcggaaacc atgggtccca tctggctgtc cgggtcgcgc ccggggccga
    19261 gcttgatggc ccgcgcctgc tcggcgatgc cctcggccac ccgctcgaag atgctctcgt
    19321 gcacgtacag gcgtgagccg gcgcaacagc attggccgtg attgaagaag atcgcgttcg
    19381 cggcgcccgg gatggcggtt tcgaggtcgg catcccgcag gatgatgtta ggtgacttgc
    19441 cgccgagctc cagggacacg tgtttcaggt cgcgagcagc agcctgaacg atgagacgtc
    19501 caacttcagt ggaaccggtg aatgccacct tgtccactcc cgggtgtgct gcaagtgcag
    19561 cccccgcggt ctccccatat ccggtgatga tgttgaccac accatccggc aatcctgctt
    19621 cctgcagaag ctcgccgagg cgcagagccg acagcggcgt ttcctcggcc accttcagca
    19681 cgacagtaca gccggtggcg agggccggtg cgagcttcca tgctgccatc agcaacggga
    19741 aattccacgg aatgatctgg cctacgacgc cgatgggctc cggacgcgta tagcagtggt
    19801 attgggctcc gggcgtgtac agcaccgaca agggaatggt cttgccttcg atcttggtgc
    19861 tccagccggc catgtactgg aagatgtccg ctgccagggg aacatccgca atccgggcaa
    19921 cggccatcgg cttgccgtta tccagggact cgagctcggc gagctcatcg gcatgtttgg
    19981 cgatcagctc gccgatgcgc cacaccagct tgccgcgctc cgatggagtc agtgccggcc
    20041 agggtcctga ctcgaaggcg cggcgcgcag cctgaaccgc caggtccacg tccgccttgt
    20101 ctccctcggc aacgcgggcg atcgtcgccg ccgtcgccgg atccttgacc tcgaacgtct
    20161 tgcccgaagc ggcggcaacc cagtggccgt caatcagcag cttcttcgga gtacccagga
    20221 actgaccgac gcgcgtggac gccggggatt cttttatgac agcgctcatc ttgctactcc
    20281 tcgcagtgcg gacccctgat cggcgtcgac cagggcggtt tcacgtgccc gcggggcaca
    20341 ccgcgcaggt cacgtgggga aaaaaagcga ctatagcgct gatcgccgat gcccagaatt
    20401 acagcggcat tacagaatca ttcatcggat tctgttccgt tctgcgctca gaggcgccct
    20461 ggaaccttgg ccgccagctt tggaacagat cttgctagat cgcaacaatc ttaggattgg
    20521 gaccgttccc ggctgcggct ccatcggcat ggctgacgtc gagcgcgata ttttgggagc
    20581 ggaccgaaag gaccccgagc ccgcggccgc agatccgaaa cctgcacttc ccggtggcgc
    20641 cccgctgccc gcgggcgaca ttgcccgtgc cagccccttc agcgacgcgg aagccctgcg
    20701 ggggcacctc gcggccatcg tggcctcatc cgatgatgcc atcatcagca agacgctgaa
    20761 cggggtcatt ctctcgtgga ataagggcgc ggagcgcatg ttcggctata cctcggagga
    20821 ggccgtcggg aagccgatca ccatcctgat tccggcggag cagctcgacg aggagccggt
    20881 gattctggcc aaagtccggc gcggcgagcg gatcgatcac tacgagaccg ttcgtgtcag
    20941 gaaggatggg ggccgcctgt atgtctcgct tacggtctcg cccgtgcgag atggcagcgg
    21001 caggatcgtg ggcgcttcga agatcgcacg cgacatcaca catcagaaga gcgcggagtc
    21061 gcaggtccgt gcgagcgagc agcgcttccg gctcatggcc gactcggccc cggtgctcat
    21121 ctggatgtcc gatgccagcc gggcgcgcac ctggtgcaac aaggcgtggc tcgacttcac
    21181 cggtggcacg ctcgaacacg agctcggatt cggctggagc gagcatgtgc atccggatga
    21241 tctcgccggc tacctgcgta cctatgttga gagcttcgac gtctgcaagc ccttccggac
    21301 cgaattccgg ctccggcggc acgatggcga gacccgttgg atgatcgata ccgccgttcc
    21361 cctcagagaa ggcccggtcg gaacattctc cggctacatc ggctcgtgta tcgacatcac
    21421 cgagtatcgg cagtcccagc tcgatcgcga ggatctgctg cgggcggagc gcgcagcccg
    21481 caccgaagcc gagcgtctca gccgcgtgaa ggacgagttt ctcgcgacac tgtcgcatga
    21541 gctgcgcacg ccattgaacg ccattctcgg ctggtcgacg ttgttgcgtc gtgtcggccg
    21601 cgagagcccg gactttgccc gcggcctcga aacgatcgag cgcaacgctc gtgcgcagtc
    21661 acaaatcatc ggcgaccttc tggacatgag ccgcatcatc tctggcaagg tgcagctcga
    21721 cattcagact gtcgatcctc acgaggtgat cgaagctgcc ctggaaagcg tgcggccttc
    21781 ggccgaggcc aagggcctgc gagtgcgcat gcgagtgcaa ggccgggtgg agctcattcg
    21841 ggccgatccc agccgattgc agcaggtgct gtggaatctg ctgaccaatg ccgtcaaatt
    21901 caccccgcct cgaggcctga tcgatgtgac ggtgaaacgc gtcaatgggc atgtggagat
    21961 cgtggtcgaa gacaccggaa tcggcgtcaa gcccgaattc ctggatctta tcttcgatcg
    22021 cttccggcag gccgatgcat cgacgacccg gcgccatggc ggtctgggcc tcggactctc
    22081 gatcgtgaag caactcgtgg agttgcatgg cggctcggtg tcggtgaaga gtcccgggct
    22141 ggaccagggc accacattca ccgtcatgct accgatgtcc gcgacgaggg ccgatgacga
    22201 taccgttcgg cagccaagac cgactttcgc ggacgtcgat tttgccgccg ttgatctacc
    22261 gagcctgtcg ggcgtcaagg tgctcgtggt ggacgatgaa cccgactcgc gcgtgctggt
    22321 cagcaggctg gtcgaggacc gcggagccct cgcgatcccg gcggcaagcg ctgaagaggc
    22381 attgcacagg atcgtcaagg agccggttga catcgtggtc agcgatatcg gcatgccgga
    22441 agtggacggt taccagctca tgcggcagat ccgcggtctc gagcgggaga ctctgcgtgg
    22501 tgtcatggcg atcgccctca ctgcttacgc gcgcccggcg gatcgccagc gcgcgctgct
    22561 tgcggggtat cagatgcacc tgtgcaagcc agtggatccg cgcgagctgg tcgtcggcat
    22621 cgcgagcctg ctcaacattc cgaagcagat ggcacattaa cgctgtgcag cgcaaccggc
    22681 gcggcggcgc ccaggcgccg ccgcgccggc agtgtcagct ggtcttcata tccagtgact
    22741 tcatcatgtc cgtcgccttg tccttgaccg cctggccctt ggccactgcg cccgcatagt
    22801 cttcgttgga tacggcgttc gtggcgtccg tccactgaga gtcaagcgct gccacgccac
    22861 tcttcgccga ctcgagggat gccttggtca ctcccttggg caacctgtgg gattttgaca
    22921 gggtgtcgac ctgtgccttg atggcatcca ccatcttggg cacatccgtg ctcagtgttc
    22981 gccactgctg cttcgtctgt gcaagggccg catcggctgc ggcctgcttg gtgtcggcgt
    23041 cctgcttgag gctggtgatc gccgtattta ccgccggtgc cgccgccagc acctccttat
    23101 aatccccctt ggccagattc cccttgagtg tcgcgacctg gccctcgacg ttctgcagca
    23161 tctccggaga atatttggcc gcggtgtcgt ggaccgcgcc gagcgaagta tcgatgtccg
    23221 caacagcctg ctccgcgggg gccttctgac tggaacaggc gaacatgagc agagtcgcga
    23281 cgcctgccgc aagccaggcg cctgcagcaa gccgattgga attattcatg aatcgaactc
    23341 ctcgtgatga ctgacgggcg tctcgccgcc ggccggcctc aactgcactc ggtcacgccg
    23401 actgcgatcc tgcggcctgc aaggctaaac ccgccccgcg tcaatgacaa atgggccgcg
    23461 cgggctgccg ggaagttgga tgagccgctt tatcgcgccg ctcgtgttct cataagttat
    23521 tgtaaatcag tggcttatga tcagactaat ccccgcccat cgctgtcatg gccgctctca
    23581 cggctgccgc acagtttcgc tgcagagcgt accggattgt cgcagccagt tggcccgggc
    23641 gcgcctcagc tgcgcaccgc gcgacgtgca tgcatccgac atcacgatga ttgacgccga
    23701 cacactcgag cgagcccgcg ggcagacaat acctggcaga tgcgcgcaat gagctggcat
    23761 gcgcagacac tcgcggcggt caccgactac gacgatgcgc cgctgcggcc atttcacttg
    23821 cgagttgcga tcgcgagcac gggtggtgcc ttcgcggacg ggttcgggct ggggatcatt
    23881 ggaatcgcgt tgagcctggc cgcccccgag ctcggcctgg gagcgctgtg gcaggggctg
    23941 ctcggcggcg cttcactggc cggattgttt gccggcgctc tgttgaccgg tccggccgcc
    24001 gaccgcttcg gtcgccgacc cttcttcgct tacaacatgc tgatcgccgc agtgatctgc
    24061 gctctgcagt acaccgcgcg cagcggtcag gagctcctgc tgctgcggat cgcgatcggc
    24121 tacctgctcg ggaccgatta cgtcgtcagc aaggcgctgt tgagcgaatt cgcgccgctg
    24181 cggttgcggg gccggatact gggagtgctc tccatagcct gggccggcgg gtatgcgtgc
    24241 gcctatgcat cgggctttgc gctcacagtc gtcggccatg aggcgtggcg ctggatgctg
    24301 ctggccagtg ccctcccatg tctgctgatc acgccattga ggctcacgat gccggagtcc
    24361 cccgtgtggc tcgtaaagca cggccagccg gagcgcgcag cagcggtcgt ccacgcaaag
    24421 ctcggcgaga acatacgacc accccgcggc accccggcac gcacctcgca ggccggtcgt
    24481 tggaggcagt tattcggccc gcgctggcgg ttgcgcacgc tggtggcctg cacattcttc
    24541 acgtgccagg tgatccccta ctttgcggtc ggtacttt
//
LOCUS       DASRHN010000213         4593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1718600, whole genome shotgun sequence.
ACCESSION   DASRHN010000213 DASRHN010000000
VERSION     DASRHN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718600"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1068
                     /locus_tag="VFB37_08215"
     CDS             256..1068
                     /locus_tag="VFB37_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZO22478.1"
                     /translation="MKRVQRRARNGDTRDPLFNQSVEKALSILEIFGGERRAMNLGEI
                     AAAVGVTKSSAQRCTHTLERLGYLRRDARVKRWVLTPRSLSVAHAYLSGHTLLEQATT
                     HLIDLNQASGESVSLSEPDETDMVFVARFPSHKRFFIHMPVGRRLPMYCTASGRAYLS
                     ALPAAEAQRLLRRSSLRQFTPTTLTDPAEILRHIGLAREAGYAWSDQECYRGDVTIAA
                     AVLGEDGRPVAAINVSAPTSRWSLAELRSKLSSVLLQTARAASGGIAPRLRA"
     gene            1108..1251
                     /locus_tag="VFB37_08220"
     CDS             1108..1251
                     /locus_tag="VFB37_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22479.1"
                     /translation="MLAATLAPETVFAFLVEASGALMMFVYIIVRARRESARGPQLAR
                     PPG"
     gene            1288..2637
                     /locus_tag="VFB37_08225"
     CDS             1288..2637
                     /locus_tag="VFB37_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO22480.1"
                     /translation="MAIVDPGRLEALHAAELGRFTARTRRSAQLLERGRHSMIKGVPM
                     PWMWGLYRHPPIFAARGSGACFVDVDGNTYLDFNVVDLAMTMGFGDGPIAEAVHEAMQ
                     RGAHFLLPVEEALCVSELLAERTGVPFWQFTLSASGANTEVIRIARSLTGREKIVVFR
                     GHYHGHVDDTLVDEEPGRLQPAVMGLPRGTEHRLIIVPFNDLAALEAALDPQDVALVL
                     SEPALSNCTLVHPEPGYLAAVYAACQRSGTLFCLDEAHTFQFAYGGLKREWDLACDFL
                     VLGKGLGTGVSFGLYGMSQAVAQGVEARTARDLGEPGIAAGGTTYASTIAVLAARAAL
                     EKVLTPASYERVQALGKRLASGLDALFNALGLPWRAQHLGPRSGFCLFPQLPRNGTEA
                     LRSVQIGFIAARKLWMANRGVWDAMPTAGPQVSFVHTADDVDTYLRTVRPFLEAIIR"
     gene            2793..>4593
                     /locus_tag="VFB37_08230"
     CDS             2793..>4593
                     /locus_tag="VFB37_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZO22481.1"
                     /translation="MHQPEPASGEVRREPADSRTARAIDRRGFLKTSAAISGGLLIQV
                     LIAGGRGLARADTSSRGSLPEAGAKPAVLNAYIRIDPDGAIVLIMPKVEMGQGTFTSL
                     PMLVAEELEVPLGALSLEHAPPDPAVYGVEGDQSTGGSTSIRDCWLPLRKAGAAARLM
                     LIQAAARGWGVAPSSCRAEQGAVIHDSSARRASYGSLASAAATLPMPADPPLKAAKEF
                     KVIGRATPRRDTPDKTHGRTVFGIDAQVPGMRIAILALSPAEGGHVVEPLNTSAAMAV
                     RGVRQVVNDQGDALAVVADDTWSALRGLKALGLRWDAGHNGSVQQTAIVAELEAAAKQ
                     PGAIAAHAGNAQEAIKRATSRVDAVYHQPFLAHATMEPMNCTVHWHEDHCEIWVGTQA
                     PDRAVAKLAALGLKPEQIILHNHLIGGGFGRRLEVDGIVIAARIARHVQGPVKVLWTR
                     EQDVQHDRYRPYYVDRISAGLDAHGKPLAWLHTIAGSSVSERWSGEPNKNGVDDDAVE
                     VAADPVYALPNLEVRYVKQEPPGVPTSWWRGVGPTRSVFVVESFIDELASAAKQDPVQ
                     YRQALIKDSRLRAVLDLAAARASWGHALPQGHGR"
BASE COUNT          730 a         1499 c         1542 g          822 t
ORIGIN      
        1 tgagccccgc gcgcagtccg aaagcggctg caccggtggc tgccatcagt gtcaggacgc
       61 tacggcggga gtggctgttt cggggggttc tggatgatgc catggtgatc ctccggtgat
      121 gacggaatcg cgcctcgcaa ttttcgtgcc cccaagtgtg acggcgttgg tgatcgatta
      181 tcaatagccc tatactcgat ccaaactacg ctatcgcctc ggaacacccc gctgaacaag
      241 acctccgccc gggacgtgaa gcgcgtgcag cgcagggctc gtaacggaga cacgcgcgat
      301 ccgctcttca accagtcggt cgagaaggcg ctttcgatcc tggagatatt cggcggtgag
      361 cggcgcgcca tgaatctcgg cgagatcgcc gctgcggttg gggtgaccaa gagttcggcg
      421 caacgttgca cacacaccct ggagcgcctg ggctatctga gacgcgatgc ccgcgtgaag
      481 cgctgggtgt tgactccgcg ctcgctcagc gtcgcacatg cctatctgtc gggacacacg
      541 ctgctcgagc aggcgaccac acacctgatc gatctcaacc aggccagcgg cgagtcggtg
      601 agcctctccg agccggatga gaccgatatg gtgttcgtcg cgcgcttccc gagtcacaag
      661 cgattcttca ttcacatgcc cgtggggcgc cggctgccga tgtactgcac ggcctcggga
      721 cgagcctacc tctcggcttt gccggcggcc gaggcccagc ggctcctgcg gcgctcgtcc
      781 ctgcgtcagt tcacgcccac gacgctgacg gatcctgccg agatcctgcg gcacatcggg
      841 cttgcgcgcg aggcaggcta cgcctggtcg gatcaggaat gctaccgggg cgatgtgacc
      901 atcgccgccg ccgtgctggg tgaggacggc cggccggtcg cggcgatcaa cgtctcggcg
      961 ccgaccagcc gctggtcact cgcggagctg cgctcgaagc tctcttccgt gctgctgcag
     1021 accgcgcggg cggcctcggg tgggatcgca ccccgattgc gggcgtgagt tctgaagtca
     1081 ggacgcgttt gaggtcagga gcccggggtg ctcgccgcga cgctcgcccc ggagacagtg
     1141 tttgcattcc tcgtcgaggc ctcgggtgcg ctcatgatgt tcgtctacat catcgttcgg
     1201 gcaagacgcg aatctgctcg cggcccgcag ctcgcacgac ccccgggctg agtcacagcg
     1261 ctccgctcgc cagtgtgact cctcagcatg gctatcgttg atcccgggag gctcgaggcg
     1321 ctccacgccg cggagctggg tcgattcacg gctcggaccc ggcgctccgc gcagctgctc
     1381 gagcgcggcc gccattcgat gatcaaaggc gtgccgatgc cctggatgtg gggactgtat
     1441 cggcacccgc cgatcttcgc cgcccgcggc agcggcgcct gcttcgtgga tgtcgatggc
     1501 aacacatatc tcgacttcaa tgtcgtggat cttgcgatga ccatgggctt tggagacggg
     1561 ccgatcgctg aggccgtgca cgaggcgatg cagcgcggcg ctcacttcct gttgccggtc
     1621 gaagaagccc tgtgcgtctc cgaactgctc gcggagcgaa ccggcgtgcc gttctggcaa
     1681 ttcactctgt cggcatcggg tgcgaacacg gaggtgatcc gcattgcgcg ctcgctcacg
     1741 ggacgggaga aaattgtcgt cttccgaggc cattaccacg gacacgtcga tgacacgctc
     1801 gtggacgagg agcccggccg actccagccc gcggtcatgg gcctgccgcg tggaaccgag
     1861 catcggctca tcatcgttcc tttcaatgac ctggctgcac tcgaggcggc gctggatccc
     1921 caggacgtcg cgctcgtgct cagcgagccg gcgctgtcga actgcactct cgtgcatcct
     1981 gagccggggt atctcgccgc ggtgtatgcg gcctgccagc gttccggcac gctgttctgt
     2041 ctcgatgaag cgcacacctt ccagttcgct tatggagggt tgaagcggga gtgggacctc
     2101 gcctgcgatt tcctcgtcct gggaaaaggc ctcggcaccg gagtctcgtt cggactgtat
     2161 ggaatgtcgc aagccgtagc gcagggcgtg gaggcccgga cagctcggga tctcggggag
     2221 cctggtatcg ctgccggagg caccacctat gcgagcacga ttgctgtgct ggccgcgcgc
     2281 gcggcgctgg agaaagttct gacccctgcg agctatgagc gtgtgcaggc actgggcaaa
     2341 cgcctggcat ccggcctgga tgcgctcttc aacgcgctgg gcttgccctg gagggcacag
     2401 catctgggtc cgcgctcggg tttctgcctc tttccgcaac tcccgcgcaa tggcaccgag
     2461 gcattgcgct cggtgcagat cggattcatc gcggcgcgca agctgtggat ggcgaatcgc
     2521 ggcgtgtggg atgcgatgcc gacggccgga ccgcaggtgt ctttcgtcca cacggccgac
     2581 gatgtggata cttacctgcg gacggtgcgg ccctttctcg aggcgatcat ccgctgatgc
     2641 ggcaaatcgg ccgcctccgc cacggagctc cggccgcctc gagtgcctcg cattcgggac
     2701 acgtttgacg caccgcccgc gcatccccta cagtcgcggc tcgatgccga tttgacgata
     2761 tcgattatcg atcataagcg ggccaggccc agatgcacca acccgaaccc gccagcggtg
     2821 aggtccggcg cgagccagcc gattcccgga cggcgcgcgc gatcgatcgg cgcggatttc
     2881 tcaagacctc cgcggcgatc agcggcggat tgctgattca agtgctaatc gcgggcggcc
     2941 gcgggctcgc ccgcgcggac acttcatcac gcggctcgct gccagaggcc ggggcgaagc
     3001 ctgccgttct caatgcctac atccggatcg accctgatgg ggccatcgtg ctcatcatgc
     3061 ccaaggtcga aatgggtcag ggcaccttca cctcgctgcc gatgctggtt gcggaggagc
     3121 tcgaggtccc actgggtgcg ctcagcctgg agcacgcgcc gccggatccg gcggtgtatg
     3181 gcgtggaagg cgatcagtca accggcggct caacctccat tcgggattgc tggctgccgc
     3241 tgcgcaaggc gggagccgcc gcccgcctca tgctcattca ggccgcggcc cgcggctggg
     3301 gcgtcgcgcc ttcgtcctgt cgggccgagc agggcgcggt gattcacgat tcctcagcac
     3361 gacgcgccag ctatggctcg ctcgcaagtg cggccgcgac cctgccgatg cccgcggacc
     3421 cacctctcaa ggccgccaag gaattcaaag tcatcgggcg cgccacaccg cgccgggata
     3481 cgccagacaa gacgcacggc cgtacggtct tcgggatcga tgcgcaggtg cccggaatgc
     3541 gcattgccat tctggcgctg tcccctgcag aaggcgggca cgtcgtcgag ccgttgaaca
     3601 cctcggctgc catggccgtg cggggtgttc gccaggtggt caatgatcag ggcgatgccc
     3661 tcgcggtggt ggccgatgac acctggtcgg cgctgcgagg gctcaaggcg ctcggcctgc
     3721 gctgggacgc tggccataac ggctccgttc agcagactgc gatcgttgcc gaactcgagg
     3781 cggcggccaa gcagccgggc gcgatcgctg cgcatgccgg caatgcccag gaggcgatca
     3841 agcgcgcgac ttcgcgggtc gatgcggttt atcaccagcc gtttctggcg cacgccacca
     3901 tggagccgat gaattgcacg gtgcattggc acgaggatca ctgcgagatc tgggtgggga
     3961 cgcaggcgcc cgatcgcgcc gtggcgaagc tggccgcgct cggcctcaag cccgagcaga
     4021 tcatcctgca caatcatctc atcgggggcg ggttcggtcg gcggttggaa gtcgatggca
     4081 tcgtgatcgc cgcgcggatt gcgcgccacg tgcagggccc cgtcaaagtc ctctggaccc
     4141 gcgagcagga tgtccaacac gaccggtacc ggccgtacta cgtggacaga atctcggctg
     4201 gtctggatgc acacggcaag ccgctcgcct ggctgcacac gattgccggc tcctcggtct
     4261 cggaacgctg gtcgggggag cccaacaaga acggggtcga tgacgatgcg gtcgaggtcg
     4321 ctgccgatcc ggtgtacgcc ctgccgaatc tcgaggtgcg ctacgtcaag caggagcccc
     4381 ctggcgttcc cacatcatgg tggcgtggag tcggtcccac ccgcagtgtg ttcgtggtcg
     4441 agagcttcat cgatgagctt gcgagtgctg caaagcagga tcccgtccag taccggcagg
     4501 cactgatcaa ggattcgcgc ctgcgtgccg tcctcgatct cgccgcagcc cgggcgagct
     4561 ggggccacgc gctgccccag ggtcatgggc gcg
//
LOCUS       DASRHN010000214         9521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1718664, whole genome shotgun sequence.
ACCESSION   DASRHN010000214 DASRHN010000000
VERSION     DASRHN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718664"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..761
                     /locus_tag="VFB37_08235"
     CDS             66..761
                     /locus_tag="VFB37_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22482.1"
                     /translation="MGALVEIRNLSKVYERGTQKVEVLHHVNLDIDKGDFVALMGPSG
                     SGKTTLLNLIGGLDSPTDGSINVSSERIDRLGEGALAKWRASNVGFVFQFYNLLPMLT
                     ARRNVELPLLLTRLGSGQRKRNADVALQLVGLADRATHKPGELSGGQQQRVAIARAIV
                     SDPTLLVCDEPTGDLDRQSATEVLTLLQQLNREHGKTVIMVTHDPKAAEYARHTLHLD
                     KGTLVEQVGTQPA"
     gene            758..1921
                     /locus_tag="VFB37_08240"
     CDS             758..1921
                     /locus_tag="VFB37_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZO22483.1"
                     /translation="MKYLHLVFAALMRRKTRTLFTLFSVLAAFLLFGLLDSVRTFFDQ
                     AGSGVAGVDRLITISKVSFTLSLPKSLLQRIQATPGVAKVTYANWFGGIYQDPKNFFA
                     NQAVADNFFDLYPERLIPAAQLRAFHDTRTGAVVGEALIQKFHWKIGDKIPLQATIFP
                     QKGGSNTWTFDLVGIYHLADPKLKAQENALFFNWDYFDEARAFGNGNVGWYVTKVADR
                     DRANQVAQAIDALSANSDHETKTQTEQAFTAAFVSQFADIGLIVGAIMGAVFFTLVLL
                     TGNTMAQAVRERIPELAVLKTIGFSNRSVLGLVLSESAALLVLGSLLGLAIAGIIVGG
                     LRGTVGSAIPMHPIDGAIWLRGVLLAVLIGLLVGALPAMRGMRLRIVDALAGR"
     gene            1940..3253
                     /locus_tag="VFB37_08245"
     CDS             1940..3253
                     /locus_tag="VFB37_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22484.1"
                     /translation="MLRKIGLFLGAVACIVVPIAVLTVLPWWLLAIFVVLLGCWMAFT
                     RTGTQTWSVTQVGIATIPQRLGSSAVVVVGIAGVVGVLVALLAMGAGFAATLRQTGAD
                     DTAIVIRAGAQTELNSVLDHNTATLVAQAPQVLKNAQGDPIASPELVVVASLPKKANG
                     LDANVEIRGIGERAWELRPNVKIIAGRKFGLGLRELIVGKGAAAQFAGLDLGSSLKLN
                     GEQWTVVGEFDSGDANNSDLWGDTAVVGSAYRRGSSTTSVTVRLTDAAAFDAFKAGLA
                     SDRRLQVDVFTTRNYYNTQAEGLTRTIRVLGTTVGIIMAIGAVFGALNTMYAAVATRT
                     REIATLRAIGFRAVPVIVSVLLETVLLAVLGGVLGAAIAWALFDNYTASTLGSNFSQV
                     VFAFHVTPELLWNGLKWALAIGVIGGLFPAIRAARMPVTAGLREM"
     gene            complement(3328..4335)
                     /locus_tag="VFB37_08250"
     CDS             complement(3328..4335)
                     /locus_tag="VFB37_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22485.1"
                     /translation="MIQRLLSVWHARNLEFVRDRPTLIFTILLPLMIVVGMSFVFGGK
                     ERPLFKVGVLSAAIDRNAHPFLRERYVDFVPIADETEGLRKVTHQQIDLLVDLHGQPR
                     YWVNTDSPRGYIVEKLLLAAAPGAQRQPVTGAAVRYVDWLFPGILGMNMMFSCLFGVG
                     YVVLRYRKSGFLKRLHATPLSAFEFLSAQVLSRLCLILFVSVVLYAGIGTIIHFHTAG
                     SLLALLLVAAIGALSLIALGLTIAARFSSEELVGGLLNLLTWPMMLLSGIWFSLEGSP
                     QWVRWIAAIFPLTQVLDAARAIMLDGAGLYQVAPHVIYLACTALVFLAFGAWSFRWRV
                     D"
     gene            complement(4332..5288)
                     /locus_tag="VFB37_08255"
     CDS             complement(4332..5288)
                     /locus_tag="VFB37_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22486.1"
                     /translation="MSIASDPAYPDVSAAPSSSAVPAGGAPILQVRDLIKRYPSVTAV
                     DGVSFEVREGSCFGLLGPNGAGKTTTIEIMEGILMATSGEVRYRGEPLGQRFREQAGI
                     LFQKTALQDFLTVRQSLALFRGLYEHGLDVDEVIRICALEKLARRDNRKLSGGQQQRL
                     LLAIALVNDPALLFLDEPTTGLDPQARRNFWELIQSIKARKKTVILTTHYMEEAELLC
                     DEIAIMDRGRIIAQGPPRQLLREHFAEVLLELPRQDFPEEARRLPLRVLDTPERVEIT
                     TDDLDSTLRTLIEARVPLANLRIRPANLEDLFLELTGKELRA"
     gene            5440..6138
                     /locus_tag="VFB37_08260"
     CDS             5440..6138
                     /locus_tag="VFB37_08260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZO22487.1"
                     /translation="MRESRRARRLAVRVFHTGRVEVVVPSRTSPSAVERFVSRHRGWI
                     ERKCQEARSRAAPGAPFPPERIDLAASQESWRVQLVGGGRNVTLTRAEEGLLALAGDS
                     SNGRAVHAALRRWLLQRAAEVLEPMLARLSRTLGYEYRRLLIRRQRTRWGSCSSRGTI
                     SLNCCLLFQRAAVVEYLMIHELAHTRHMNHSRRFWQAVAKDCPAHRALDRELLAGWRR
                     VPAWVFADEHGRES"
     gene            6135..6992
                     /locus_tag="VFB37_08265"
     CDS             6135..6992
                     /locus_tag="VFB37_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HZO22488.1"
                     /translation="MSTRKDNRSGHVDLSDEAVHWELGSSLSYGQYLQLDKILEAQKP
                     LTYEHDEMMFIIVHQTSELWMRLMLQELRGALECIRRDDLDPSFKMLSRISRVQAQLI
                     STWDVLSTMTPSEYSAFRNALGRSSGFQSVQYRTLEFLLGNKHAQLIEVHKHDESAYE
                     VLRRALESPSLYDECLRLLSRRGYEIPESHLTRDFSESYVPSKQVAGAWLAVYHNAEK
                     DWDLYELAESLVDLDQKFQLWRCHHLKTVERIIGYKPGTGGTSGVSYLAKALELKFFP
                     ELWQIRTSM"
     gene            complement(7015..7581)
                     /gene="msrB"
                     /locus_tag="VFB37_08270"
     CDS             complement(7015..7581)
                     /gene="msrB"
                     /locus_tag="VFB37_08270"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZO22489.1"
                     /translation="MSKLPPLSRRALLACALPLAIAASRLARAAALSSADSAEQVTIE
                     SFAADGKSRGKQSVARVLKTDTEWRSQLSLLAYTVTRHAGTEPAFSGSYDHNHADGLY
                     GCICCGTVLFDSKTKFDSGTGWPSFWQPISRLNVVEKADDSFGMQRTAVSCRRCDAHL
                     GHVFDDGPRPTGLRYCMNSVALSFTARA"
     gene            complement(7607..9469)
                     /locus_tag="VFB37_08275"
     CDS             complement(7607..9469)
                     /locus_tag="VFB37_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZO22490.1"
                     /translation="MILAVVALCGGVLTIFSPCILPVVPFVLARTERPFARSTLPLLI
                     GMAAAFAGISTLAAVGGGWAVHVNDYGRLAAMVLLTLSALALLSRRLADRITRPLVAL
                     GSRLASHPGGNTRGSIATSLLLGLATGLLWAPCAGPILGLILTGAALRGPSVDTSILL
                     LAYALGAACSLGLVAAASTRVIAAMKRSFGAGEWLRRALGAAVLIAVVAIALGWDTGA
                     LTRLSESGTNHFEQSLLDRIAPRTAHAGMMAMQGAMQADPQAPMMNARSANSDAALSA
                     DGGSMSGHPGATMTGASAGAAPPVEGELPSLDGAVQWLNSPPLTAQALRGKVLLIDFW
                     TYSCINCLRALPYVKSWYARYRDQGLVVIGVHAPEFAFEKNPTNVSRAVHDLGITYPV
                     ALDNNYAIWKAFNNEYWPAHYFIDAQGRIRGHHFGEGDYAQSEQLIRELLSEAGAQHL
                     GEVTTQAVQGNGVQASADEADVESPETYVGYERADNFVPAGGLAHDEAHAYAAPTQLR
                     LNQWALSGVWKVEGEKAVAGAPHDRILFRFHARDLHLVLGPAAGGRPVRFRVTIDGHA
                     PGADHGIDVQSDGAGTVTEQRLYQLLRQSQPQSDHTFEIEFLDAGVQAYSFTFG"
BASE COUNT         1729 a         3060 c         3091 g         1641 t
ORIGIN      
        1 cgcctgagat gagcgacggt gcgcgagtca cggtaacagc aaaatagctc gataaggagc
       61 cgaacgtggg tgcactggtc gagatccgca acctgagcaa ggtttacgag cgcggcacgc
      121 agaaggtcga ggtgctgcat cacgtcaacc tggacatcga caaaggcgac ttcgtcgctt
      181 tgatgggccc ctcgggctcc ggcaagacca ccttgctcaa cctgatcggc ggtctggatt
      241 ccccgacgga cggcagcatc aacgtgtcga gcgagcgcat cgaccgcctc ggtgagggcg
      301 cgctcgccaa atggcgagcc tccaacgtcg gctttgtctt tcagttctac aacctgctcc
      361 ccatgctgac cgcccgcaga aacgtggagt tgccactgct cttgacacga ctcggctcgg
      421 gacagcgcaa gcgcaacgcg gatgtcgcgt tgcagctggt gggacttgca gaccgggcca
      481 cgcacaaacc gggcgagctc tccggagggc agcaacagcg cgtggcgatc gctcgcgcca
      541 tcgtctccga tccgaccctg ctggtgtgcg atgagccgac gggcgatctg gaccggcagt
      601 cggccaccga ggtcctcacc ctgctgcagc aactcaatcg cgagcacggc aagacggtca
      661 tcatggtgac gcacgacccg aaggctgcgg aatacgcccg ccacactctg cacctcgaca
      721 aaggcacact ggtcgagcag gtcgggacgc agccggcatg aagtatctgc atctggtgtt
      781 tgccgcgctc atgcggcgca agacccgcac gctcttcaca ctgttctcgg tgctggccgc
      841 tttcctgttg tttggcctgc tcgactcggt gcggaccttc ttcgatcagg ccggcagcgg
      901 cgtggcgggc gtggaccggc tgatcaccat ctccaaagtg agcttcactc tgtccctgcc
      961 caagagtctg ctgcagcgaa tccaggcgac cccgggtgtg gcgaaggtga cttacgccaa
     1021 ctggttcggg ggcatctacc aggacccgaa gaatttcttt gccaaccagg cggtcgcaga
     1081 caacttcttc gatctgtatc cggagcggct gataccggct gcacagctgc gggcatttca
     1141 tgacacgcgc accggggcag tcgtaggcga ggctctcatc cagaagtttc actggaagat
     1201 cggcgacaag atcccgctgc aggcaacgat cttcccgcag aagggcggca gcaatacctg
     1261 gacgttcgac ctggttggca tttatcacct ggcggatccc aagttgaagg cgcaggagaa
     1321 cgcgctgttc ttcaactggg actacttcga tgaggcgcgc gccttcggta acggcaatgt
     1381 cggctggtat gtcacgaaag tcgccgatcg cgatcgggcg aaccaggtcg cccaggcgat
     1441 cgatgcgctc tcggccaact ccgaccatga gaccaagacc cagaccgagc aggccttcac
     1501 ggcggccttc gtcagccagt tcgccgacat cggtctgatc gtcggcgcga tcatgggcgc
     1561 agtgttcttc actctcgtgc tgctcaccgg caacaccatg gcacaggccg tacgcgagcg
     1621 catcccggag ctggcggtgc tgaagaccat cggtttttcg aaccgcagcg tgctcgggtt
     1681 ggtgctctcg gagtcggcgg cgctgctcgt gctggggagc ctgctcgggt tggcgatcgc
     1741 aggcatcatc gtcggtgggc tgcgagggac cgtcgggagt gccatcccga tgcacccgat
     1801 cgacggcgcg atctggttgc ggggtgtact gctggcggta ctcatcggat tactggtcgg
     1861 ggcgcttccg gcaatgcgcg gcatgcgtct gcgcatcgtg gatgcgctcg ccggacgctg
     1921 agctcccacg agggctgaga tgttgcgcaa aatcggtctg tttctgggcg ccgtcgcctg
     1981 catcgtcgtt ccgatcgccg tgctgaccgt gctgccctgg tggcttctcg ccatcttcgt
     2041 cgtactgctg gggtgctgga tggccttcac gcggaccggt acgcaaacct ggtccgtgac
     2101 ccaggtgggt atcgccacca ttccgcagcg gctcggctca tccgcggtcg tcgttgtagg
     2161 aattgcgggc gtcgtcggcg tgctggtcgc attgctcgcg atgggagccg gatttgccgc
     2221 gactctcaga cagaccggcg ccgatgacac cgcaatcgtg attcgcgccg gtgcccagac
     2281 cgagctcaac tcggtgctcg atcataatac cgcgacgctc gtcgcccagg cgccgcaggt
     2341 gctgaagaat gcccagggag atccgatcgc ctctccggag ctggtggtcg tggcctcgtt
     2401 accgaagaaa gccaacggac tggatgccaa cgtcgagatc cggggcatcg gcgagcgtgc
     2461 ctgggagctg cgcccgaatg tgaagatcat cgccggacgc aaattcggcc tgggcctgcg
     2521 ggagctgatc gtcggcaagg gtgccgcggc gcaatttgcc ggcctcgatc tgggctcgag
     2581 cctcaagctc aatggcgagc agtggaccgt cgtcggagag ttcgactcgg gcgatgccaa
     2641 caactccgat ctctggggcg atactgcggt ggtcggctcg gcatatcgcc gtggcagctc
     2701 gaccacatcg gtgaccgtgc gcctgacgga tgccgccgcg ttcgatgctt tcaaggcagg
     2761 cctggcgagt gaccgacgcc tgcaggtgga tgtcttcacc acccgcaatt actacaacac
     2821 gcaggcggaa ggcctgacgc gtacgatccg ggttctcggc accaccgtcg gcatcatcat
     2881 ggcaatcgga gccgtgttcg gtgcgctcaa taccatgtat gcggcggtcg caacacgcac
     2941 gcgagagatc gcgacgctgc gggcgatcgg ctttcgcgca gtgccggtaa tcgtctccgt
     3001 cctgctcgag accgtcctgc tggccgtact gggcggagtg ctgggcgccg ccatcgcgtg
     3061 ggccctcttc gataactaca ccgcctccac gctgggctcg aacttcagtc aggttgtgtt
     3121 tgcattccac gtgacgcccg agctgctgtg gaacggactg aagtgggcgc tggcgatcgg
     3181 ggtgatcggc ggcctgtttc ccgcgattcg cgcagcacgg atgccggtca cggcgggtct
     3241 gcgcgagatg tagccggcgg gccgacgcgc tgcgcccgaa gcgctgcgcc aatgcaccgc
     3301 gaaagctgag gaggcagaag gcctggctca gtcgacccgc cagcggaaag accacgctcc
     3361 gaaggccaaa aacacgagcg cggtgcaggc gagataaatc acgtgcgggg caacctgata
     3421 cagtccggcg ccatcgagca tgatggcgcg tgccgcatca agcacctgcg tcagcgggaa
     3481 aatggccgcg atccatcgca cccactgcgg ggagccctcg agcgagaacc agattcccga
     3541 cagcagcatc atcggccagg tgagcaggtt cagcagccca cccacgagct cctcgctcga
     3601 gaaacgcgca gcgatcgtca ggcccagggc gatcagtgac agcgcgccga tggcagcgac
     3661 cagcagcagc gcgagcagac tgcccgcggt atggaagtga atgatggtgc cgatgccggc
     3721 gtagagcacc acgctcacga acaggatcag acacaggcgc gagagcacct gcgcgctcag
     3781 gaactcgaat gcgctcagcg gcgtcgcatg caggcgcttc aggaatccgc tcttgcggta
     3841 tcggagcacg acgtagccga cgccgaacag acaactgaac atcatgttca tgccgagaat
     3901 gccggggaag agccagtcca cgtagcgcac cgcagcgccg gtgacgggct gccgctgtgc
     3961 gccgggggcc gcggccagca gcagcttctc cacgatgtag cctcggggag agtcggtatt
     4021 gacccagtac cgtggctgcc cgtgcaggtc caccagcaga tcgatctgct gatgcgtgac
     4081 cttgcgcagg ccctccgtct catccgcaat cggcacgaag tccacatacc gctcgcgcag
     4141 gaacggatgc gcattgcggt caatggctgc gctcagcacg ccgaccttga acagcgggcg
     4201 ctccttgccg ccgaacacga agctcatgcc gacaacgatc atcagcggca gcagaatagt
     4261 gaaaatcaag gtcggccgat cgcgcacgaa ctcgaggttt cgtgcatgcc acaccgagag
     4321 cagccgctgg atcatgcgcg cagctccttg ccggtcagct ccaggaacag atcttccagg
     4381 ttcgccggcc ggatgcgcag gttggcgagc ggcacgcggg cctcgatgag cgtacgcagc
     4441 gtcgaatcca gatcatcggt ggtgatctcc acgcgctcgg gcgtatcgag cacgcgcagc
     4501 ggcaggcgcc gcgcttcctc gggaaaatcc tgccgcggca actccagcag cacctcggcg
     4561 aagtgctcgc gcagaagctg tcgcgggggg ccctgggcga tgatcctccc gcggtccatg
     4621 atggcaatct catcgcacag cagctcggct tcctccatgt aatgggtcgt caggatgacg
     4681 gtcttcttgc gggccttgat cgattgaatg agctcccaga agttgcggcg cgcctgcgga
     4741 tcgagcccgg tggtgggctc atcaagaaac agcagggcag ggtcattcac cagtgcaatc
     4801 gcgagcagca gccgctgctg ctggcccccc gagagcttgc gattgtcgcg tcttgcgagc
     4861 ttctccagcg cgcagatccg gatcacctca tccacatcca ggccgtgctc ataaaggccg
     4921 cggaacagcg ccagagactg gcgcacggtg aggaaatcct gcagcgcggt tttctggaac
     4981 aggatcccgg cctgttcccg gaagcgctgg cccaagggct cgcctcggta gcgcacctcg
     5041 cccgaggttg ccatgagaat gccttccatg atttcgatcg tcgtggtctt gccggcgccg
     5101 ttcggcccca gcagaccaaa gcagctgccc tcgcgcacct cgaaggagac tccgtccacg
     5161 gcggtcacgg agggatatcg cttgatgaga tcgcgcacct gcaggatcgg cgcgccccca
     5221 gcgggcactg ccgaagagga cggcgcggcc gagacatccg ggtaggcggg atcggaggcg
     5281 atggacatga cgatccggtt caagtgcaag gctggcgcgc gatcctacag cacgccgcag
     5341 gacggctggt atcctatgag cgttcagccg ctactgccga ctatgcctac gtcccagttg
     5401 ccgttatggt ccgagaggga tggcgcccag caatgggtcg tgcgcgaaag ccgccgtgcg
     5461 cggcgcctcg cagtgcgagt gtttcacacg ggcagggtcg aggtcgtcgt cccttcgcgc
     5521 acctcgcctt cggcagtgga gcgcttcgtg agccggcacc gcgggtggat cgagcgcaaa
     5581 tgtcaggagg cgaggagcag ggccgcccct ggagcgccct ttccgcctga gcgcatcgat
     5641 ctcgcagcct ctcaggagag ctggcgagtg caactcgtgg gcggcggccg aaatgtcacc
     5701 ctgacgcggg ccgaggaggg attgctcgca ctggccggtg atagcagcaa tgggcgcgcg
     5761 gtgcacgctg cgctgcgccg ctggcttctg cagcgcgccg ctgaggtgct cgagccgatg
     5821 ctggcgcggc tgtcgcgcac tctgggctat gagtatcggc gcctcctcat tcgccgccag
     5881 cgaacccggt gggggagctg ctccagtcgc ggcaccatca gcctcaactg ctgcctgctc
     5941 tttcagcgtg ccgcggtggt ggagtacctg atgatccacg agctcgccca tacgcggcac
     6001 atgaaccact cgcgccgctt ctggcaggcc gtggccaagg attgcccggc ccatcgcgcg
     6061 ctcgatcggg agctgctcgc gggctggcgg cgcgtgccgg cgtgggtttt tgccgacgag
     6121 cacggccgcg aatcatgagt acccgcaagg acaaccgcag cgggcacgtc gatctgtccg
     6181 atgaggccgt gcactgggag ctcggctcct cgctctccta cgggcagtat ctgcagctcg
     6241 acaagattct cgaggcgcag aagcctctga cctacgagca cgatgagatg atgttcatca
     6301 tcgtgcacca gacctcggag ctgtggatgc gcctgatgct gcaggagctg cgcggcgcgc
     6361 tcgagtgtat ccggcgcgat gacctggatc cgtcgttcaa gatgctctct cgcatctcgc
     6421 gtgtgcaggc tcagctcatc tcgacatggg acgttctgtc caccatgacg ccctcagaat
     6481 actcggcgtt tcgcaacgcg ctgggccgct cctcgggatt tcagtcggtt caataccgga
     6541 ccctggaatt tctgctgggc aacaagcacg cacagctcat cgaggtccac aagcacgacg
     6601 agagtgccta cgaggtgtta cggcgtgcgc tcgaatcgcc ctcgctctat gacgaatgcc
     6661 tgcgtctgct cagccgtcgc ggttacgaga ttcccgaaag ccatctcacg cgcgacttta
     6721 gcgagtctta tgtgcccagc aagcaggtcg caggtgcctg gctcgcggtt tatcacaacg
     6781 ccgagaagga ctgggatctg tacgagctcg ccgagagcct cgtcgacctc gaccagaagt
     6841 tccagttatg gcgctgtcat cacctgaaga cggtggaacg gatcatcggc tacaagccgg
     6901 gtacgggcgg gaccagcggg gtgtcctacc tggccaaagc gctcgagctg aagttcttcc
     6961 ccgagctgtg gcagatccgc acctccatgt aggagcgagc ggtcggctgc gatatcaggc
     7021 gcgcgccgtg aaggagagcg ccaccgaatt catgcagtag cgcagcccgg tcggccgcgg
     7081 cccatcatcg aacacgtgcc cgagatgcgc gtcgcagcgc cggcaggaga ccgcggtgcg
     7141 ctgcatgcca aaggaatcat ccgccttctc cacaacgttg agccgcgaga tcggttgcca
     7201 gaaactcggc catcccgtgc ccgagtcgaa cttcgtcttc gagtcgaaca gtaccgtacc
     7261 gcagcagatg cacccataga gcccatcggc gtgattgtga tcgtacgagc cgctgaaggc
     7321 gggctctgtc ccggcatgac gggtcacggt gtaagccagc agcgaaagct ggctgcgcca
     7381 ctcggtgtcg gttttcaaca cccgggccac gctctgtttg cctcggctct taccatcggc
     7441 agcaaaggat tcgatcgtga cctgctccgc ggagtcggcg ctcgaaagcg ctgcagcgcg
     7501 cgccagccgg cttgccgcga tagccagggg cagcgcgcaa gcaagcagcg ctcggcgcga
     7561 gagcggcggg agtttgctca tcgggtcatt catggctgta gcctcatcag ccgaaggtga
     7621 aggagtaggc ctgcactccg gcatccagaa attcgatctc gaaggtgtga tcactctgcg
     7681 gctgggactg tcgcaggagc tggtagagcc gctgctcggt caccgtgccc gcaccatcgc
     7741 tctgcacatc gataccgtgg tcggcccccg gcgcatgtcc gtcgatcgtg acccgaaacc
     7801 ggaccgggcg accgcctgcg gcgggcccga gcaccaggtg cagatcgcgc gcatggaagc
     7861 gaaacaggat ccgatcgtgc ggcgcgccgg ccacggcttt ctcgccttct accttccaga
     7921 caccggacag ggcccactgg ttcagtcgca gctgcgtggg agcggcataa gcgtgcgcct
     7981 catcgtgcgc caggcctccc gcgggcacga aattgtccgc gcgctcgtag ccgacatagg
     8041 tctccggcga ctccacatcc gcctcatcgg cggaggcctg caccccgttc ccctgcacgg
     8101 cctgtgtcgt gacctcgccc agatgctgtg caccggcttc ggagagcagc tcgcggatga
     8161 gctgctcgga ctgcgcgtag tcgccttcgc cgaaatgatg tccgcggatc cgcccttgcg
     8221 catcgatgaa gtagtgggcg ggccagtatt cgttgttgaa agccttccag atcgcgtaat
     8281 tgttgtcgag cgccaccgga taggtgattc ccagatcgtg tacggcgcgg ctgacgttcg
     8341 tcggattctt ctcgaaggcg aattccggtg cgtgcacgcc gatcaccacg agtccctgat
     8401 cgcgatagcg cgcataccag ctcttcacgt acggcagcgc gcgcagacaa ttgatgcacg
     8461 agtatgtcca gaagtcgatc aggagcacct tgccccgcaa ggcctgggcc gtgaggggag
     8521 gggagttgag ccactgcact gcgccgtcca gcgagggcag ctcaccctcc acgggcggag
     8581 cagcgcccgc gctagcgcca gtcatcgtgg cgcccgggtg accgctcatc gagccgccgt
     8641 ccgcactcag cgcagcgtcg gaatttgcgc tcctggcatt catcatcggg gcctgtgggt
     8701 ctgcctgcat ggctccctgc attgccatca ttccggcatg ggcggtgcgt ggcgcgatgc
     8761 gatcgagaag tgactgctcg aagtggttgg tgccgctctc ggacagcctc gtcagggcgc
     8821 ccgtatccca tccgagcgcg atcgccacga ccgcgatcag cacggcggct cccagggcgc
     8881 ggcgcagcca ttctcctgcg ccaaacgagc gcttcatcgc cgcgatcacg cgcgtgctcg
     8941 ctgccgcgac caggcccagc gagcaggccg cccccaaagc gtaggcgagg agaaggatac
     9001 tggtgtcaac actggggccg cgcagcgcgg ccccagtgag gatcagaccc aggataggac
     9061 ctgcgcacgg cgcccagagc agccccgtag cgaggcccag cagcagcgat gtggcgatgc
     9121 tgccgcgggt attgccgcct ggatggctcg ccagacgtga gccgagtgcg accagcggtc
     9181 gggtaatccg gtccgcgagc cggcgcgaga gcaatgcgag cgcactcaac gtcaacagca
     9241 ccatcgcggc caggcgcccg tagtcgttga catgcacggc ccagcctccc ccgacggcgg
     9301 ccagggtcga gatgccggca aaagcggcag ccatcccgat cagcagggga agtgtgctgc
     9361 gcgcaaaggg tcgttccgtg cgcgcgagca cgaacggaac cacgggcaga atgcacgggc
     9421 tgaaaatcgt cagcacgccg ccgcataacg ccacaacggc cagaatcatt cacgctccga
     9481 gatgagggct cgaatcaccc atattccgac ggtctgacgg c
//
LOCUS       DASRHN010000215         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_402290, whole genome shotgun sequence.
ACCESSION   DASRHN010000215 DASRHN010000000
VERSION     DASRHN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402290"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFB37_08280"
     CDS             complement(<1..456)
                     /locus_tag="VFB37_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HZO22491.1"
                     /translation="MWIDGTSFNTGGAIVNAALRALSRQSDLLLVVGIVCILLLLFAP
                     IPPWLLDFLLLSNFSFALLLLLLTFYVGKPLEFSTFPSLLLIATLFRLGLNLSATRLI
                     LTSANAGHVIGAVGEHVVAGNYVIGLIVFTVLIVVQYVVVTNGAQRVAEV"
     gene            complement(482..1603)
                     /locus_tag="VFB37_08285"
     CDS             complement(482..1603)
                     /locus_tag="VFB37_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HZO22492.1"
                     /translation="MAGQEQDQDRTEPATPFKREEARRKGQVAKSLDTNSVFMLAAAL
                     ASAALWGRRFISDGAEGFRNLLGHAEEFPFEPAGLMAWMDSLFREVAHSLAPFFLLLI
                     LVGVLCNLLQTGPVFSFHPLKPDPQRLNPVQGFKRIYSMKALFETGKSLVKLGLFATV
                     AWGAIVSLLPGILAMLGAEPRGYPLLLLGFAQSLAFKLTLAMLIVALLDLAYVRWDFA
                     KKMRMSRREQKEEVKRREGDPHIRAKRRELQREAAKRSASLRRVPDADVLITNPTHLA
                     IALRYERGRALAPRCLAKGAGETALRMRIMAERHGVVIVEQRPLARALFEEVAIDALI
                     PESLYEPVARVYAEVVAVKRSRSAAPEAPTPQTTLEVRS"
     gene            complement(1614..>2365)
                     /locus_tag="VFB37_08290"
     CDS             complement(1614..>2365)
                     /locus_tag="VFB37_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZO22493.1"
                     /translation="PWLMVTLLVSLRLGAVMLMTPVFASLGMPLQVRVLLVLALSATL
                     VSGMTPELLTTAPASAADLALAAANELVVGSLLAFALFAGFAAFQFAGRIMDIQMGFG
                     VAGLLDPATRNRAPLLGMLLNLTAVMTFFAIGGHRLLLRGLAFSLERIPPGSRLTALP
                     IDTVIEQFGGMFVYAALLAAPVMTVILLVDIGMAMMARTMPQMNVFIIGLPLKILVGL
                     VVLAISLGFIGPAFVRVFDGLFAGWQKIITH"
BASE COUNT          525 a          810 c          672 g          358 t
ORIGIN      
        1 gacctcggca acccgctgcg cgccgttggt caccacgaca tactgaacga cgatgaggac
       61 cgtgaataca atgaggccga tgacataatt tcccgccacg acgtgctcac cgacggcgcc
      121 gatgacatga ccggcgttgg cgctggtcag gatcagtcgc gtggccgaca gattcaggcc
      181 cagccggaac agggtcgcga tcagcaacag cgaaggaaat gtcgagaact cgagcggctt
      241 gcccacatag aacgtcagca acagcagcag cagcgcgaaa ctgaaattcg acagcagcag
      301 aaagtccaac agccagggcg gaatcggcgc gaacaataac agcagaatgc agacgatccc
      361 gacgaccaga agcagatcag actgccggct cagcgcacgc agagccgcgt tcacgattgc
      421 acctccagtg ttgaacgagg tgccgtcgat ccacatgccc ccaatccctg tgctctcgcc
      481 ttcacgaccg cacctccagg gtcgtttgag gtgtcggcgc ctcgggcgcc gcagatcgac
      541 tgcgtttgac ggccacgacc tccgcataaa cccgggcaac cggctcgtac aacgactccg
      601 gaatcaacgc atcgatggcg acctcctcga acagggcgcg ggccaaaggt cgttgctcca
      661 cgatgaccac accatgacgc tccgccatga tccgcatgcg caacgccgtc tcaccggccc
      721 ctttcgccag acatcgcggc gccagggccc ggccccgctc gtatctcaac gcaatcgcaa
      781 ggtgtgtcgg gttggtgatc agaacgtccg catcgggcac ccggcgcaac gaggcgctgc
      841 gttttgccgc ctcacgttgc agctctctgc gcttggcccg gatgtgaggg tcaccctcac
      901 gccgcttcac ttcctccttc tgctcacggc ggctcatccg cattttcttc gcgaaatccc
      961 agcgcacgta cgccagatcc agcagcgcca cgatgagcat cgccaaggtc agcttgaacg
     1021 ccagcgactg cgcaaaccca agcagcagga gcggatagcc ccgaggctcc gctcccaaca
     1081 tcgccagaat tcccggcagc agactgacga tcgcgcccca ggccaccgtt gcgaacaacc
     1141 ccagcttcac gagcgacttg cccgtctcga acagggcctt catcgagtag atgcgcttga
     1201 atccctgcac cggattcaac cgctgcgggt ccggcttcaa ggggtgaaag ctgaagaccg
     1261 gccccgtctg cagcaggtta cagagcacgc cgaccagaat gagcagcaga aagaacggcg
     1321 ccagcgaatg cgccacctcc cggaacaaac tgtccatcca cgccatgagc ccggcgggct
     1381 cgaacggaaa ttcctccgcg tggcccagca gattgcggaa cccctccgcc ccatcgctga
     1441 tgaagcgccg cccccagagc gcggcgctcg ccaacgccgc cgcaagcatg aacaccgagt
     1501 tggtgtccag gctcttggcg acctggcctt tacggcgcgc ttcctcgcgc ttaaagggcg
     1561 tcgcaggttc cgtcctgtcc tgatcctgtt cctgtccggc catcgtcaaa tcgtcagtgt
     1621 gtaattattt tctgccagcc tgcgaacaac ccatcgaaca ctcgcacgaa cgccggcccg
     1681 ataaacccca gtgagatggc caggacaacc agccccacca gaatcttcag gggcaggccg
     1741 atgatgaaga cattcatctg tggcatggtc cgcgccatca tcgccatgcc aatatccacc
     1801 agtaaaatga cagtcatgac gggagcggcc aacagcgcgg cgtacacgaa catccccccg
     1861 aactgctcga tgaccgtgtc gatcggtaga gccgttagtc tcgatcccgg cggaatccgt
     1921 tccagcgaga aggcgagtcc ccgcaacagc aggcggtgac cgccgatcgc gaagaaggtc
     1981 atgaccgcag tcaggttgag tagcattccc agcagtggcg cacggttacg cgtggccggg
     2041 tccagcagcc cggccacacc gaagcccatc tggatgtcca tgattctgcc ggcaaactgg
     2101 aaggccgcga atccggcgaa cagcgcgaag gccagcaggc tccccaccac cagctcattg
     2161 gccgcggcca gggccagatc ggccgcggag gcgggcgcgg tcgtgagaag ttccggtgtc
     2221 atgccgctca cgagggtggc cgagagcgcc aacaccagca gaacacgaac ctgcaggggc
     2281 attcccagcg aggcgaagac cggcgtcatc agcatcaccg cgcccaagcg cagtgacacc
     2341 agcaatgtca ccatcaacca gggga
//
LOCUS       DASRHN010000216        15313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_72256, whole genome shotgun sequence.
ACCESSION   DASRHN010000216 DASRHN010000000
VERSION     DASRHN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72256"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..180
                     /locus_tag="VFB37_08295"
     CDS             31..180
                     /locus_tag="VFB37_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22494.1"
                     /translation="MYGAGGNKVYVFPAERAVVVITTTNFHVSGAGALTDKLLTDEIL
                     PMLRD"
     gene            372..2465
                     /locus_tag="VFB37_08300"
     CDS             372..2465
                     /locus_tag="VFB37_08300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO22495.1"
                     /translation="MRTSRRHVCGLLALALTIGSFGSAMAAGKRLELDDVAKMVRVSD
                     AQISPDGRSIVFVVARPNIREARYDRSLILVDVESGKQRTLTYDRRGVSNPRWSPTGD
                     RIAFLDPVQLKPEEKEDGAAKAEQSPPEPAPPNEPKYQVFVLPMNGGEAQRITHSGEN
                     VEQVEWSPDGTQMAYVTADENPNRKEIAKHNDAFEVGDNDYLATEAEMPSHLWLVPAE
                     GGPARRLTSGSWSLPKAAPPSSPASPLSWSPDGKSILIVQQATPNWGDSDQSVIAAVD
                     VASGRIRKLTAHKALEGYPLYSPDGASVAYWYPRDGDPNNGTELFLTSAEGGNGIDLT
                     HELDRNIVRAIWNRGGDALLVAAHDRVRVGLWYQPRKGNAKRLDLGDVDPSWSFWADV
                     TLGKNGALALAGSTPSHPNEIYYVPPGGGAPKCLTSFNQEIAAREIGRAEELTWKGAD
                     GVTEDGVVVYPPEFTPAHKYPLVLVIHGGPQASSALSFSALSQLLAARGYVVFSPNYR
                     GSDNLGNAYEHAIFNDAGAGPGRDVMAGLSALEAKGFVDTSRIAVSGWSYGGYMTSWM
                     IGHYHIWKAAVSGAAVNDLIHEYALADFNVTERYIFGGPPWSEKLLQAYREQSPISYA
                     WQITTPTLILSDTGDARVPISQSYLMYHALRDHGVTVQFYAYPVPGHFPGDPVRSTDV
                     YRRWIEWIDRYLRKP"
     gene            2630..3568
                     /locus_tag="VFB37_08305"
     CDS             2630..3568
                     /locus_tag="VFB37_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO22496.1"
                     /translation="MTLAGQRLYPQNALGAETSTGSAAQPNASAAGTLRSGDLTVNRI
                     GYGAMRITGDGIWGAPADVPEARAVLRRAIELGVNLIDTADAYGPDVSEQLIYESLHP
                     YPAGLVIATKGGFTRPGPDQWVADGRPEHLRAACEGSLRRLHLERIDLYQLHTPDPNV
                     PFEDSIGELVRLREQGKIRYIGVSNVTLAQLKRAQALAPIVSVQNRYNVGDRGNEAVL
                     QYCASQRLAYLPWAPLGGRRSRRRRILEALWSSSAERVAQAHSVSTARVVLAWLLQHS
                     SALLPIPGTSSRAHLEDNVAAGTLRLTPAELAELQS"
     gene            3580..3843
                     /locus_tag="VFB37_08310"
     CDS             3580..3843
                     /locus_tag="VFB37_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22497.1"
                     /translation="MRPTHYEHIATNGFTTVTRSVGLIVLVGWCGFALAQTSPAKQTE
                     AQQRVQQKILQQKKLECAKLGKYAVASTIRGTTLYKCAARPNG"
     gene            complement(3889..4113)
                     /gene="csrA"
                     /locus_tag="VFB37_08315"
     CDS             complement(3889..4113)
                     /gene="csrA"
                     /locus_tag="VFB37_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HZO22498.1"
                     /translation="MLILTRRVGETLMVGNDVTVTVLGVKGNQVRVGINAPKSVAVHR
                     EEIFVRIQRENSAATQAPPDGAGGEAPRAS"
     gene            complement(4245..4472)
                     /locus_tag="VFB37_08320"
     CDS             complement(4245..4472)
                     /locus_tag="VFB37_08320"
                     /inference="COORDINATES: protein motif:HMM:NF023510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HZO22499.1"
                     /translation="MLASAPHVQRKIIGYHQDDEGHWVAELECGHGQHVRHDPPWVNR
                     PWVTTAQGRAAALGEKLDCRKCLEGPPGTGT"
     gene            complement(4546..6528)
                     /locus_tag="VFB37_08325"
     CDS             complement(4546..6528)
                     /locus_tag="VFB37_08325"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZO22500.1"
                     /translation="MSLTQSGRLHLGWVRAAASALSIGLLASACGGGGGSSSTTTPPP
                     PPPQTYTIGGSISGLNGSGLVLQDNGGDNLTVASGSTSFTFATALASGSKYAVTVMTS
                     PSGQKCAVSNGSGTVSGANVTNVAVSCTSATTTFTVGGTISGLSGNGLVLQDNGGNNL
                     TVASGATKFTFSQALTNGAAYKVTVSASPPGQKCTVTNGSGTVAGANVTTVAVACTTT
                     AYSVSGTVTGLTGSGLVLQDNGGNNLAVAAPTGSSANFTFTGTLLNGAAYAVTVLTQP
                     TAPVQRCTVTNGSGKIAGASVTNVQVACARVGQYVFVANNADGAQGDITSFTINAQTG
                     ALTPVGGVGTPTLFPADVGPNGVIVDPSGQFVYVSNGGSDDVSREMLNSDGSLTLQGQ
                     FSTTSTVPVALAITPADTFLYASGHGTSNPGVILGFSVNPLSGALIGVVNPNAPFPFS
                     NPQLGIAIDPTSSLVFSAGDPHFLYVNAIAADGTLTQTVGSGASDASLEGINGVAVWP
                     KGGSSGGYVYTSNNLSNNVGVFSYDSTGNVTLVGSAPAGNGTAGIAIDATGKYLYVTN
                     YTDGTLSAFSINAQTGALTAIGSAVATGNVTAVANPGPIDIKIDPSSQFLYCVNKIDG
                     SVSLFTIGATGALTLVNSYVSAPDGHGAGSNALAVY"
     gene            complement(6821..7444)
                     /locus_tag="VFB37_08330"
     CDS             complement(6821..7444)
                     /locus_tag="VFB37_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22501.1"
                     /translation="MRISRLTALIAVAAAHGTLIYYFCLRVHSEATLEESELEATALT
                     LLEPPLDLPRPPSQAQKVPERSRAPPVRAAAAAEPALAPAPESAARIDWMDEAQKEAD
                     DATQRQIESEDATARKAGALRAPTQAPSGWSSRRFRRPWVGWSERSRGMEITHGALLW
                     HLNEHCVVGIFIVIPGFACRIGKIEPRDDLFSHKNEDPMHFGDWKER"
     gene            complement(7525..8172)
                     /locus_tag="VFB37_08335"
     CDS             complement(7525..8172)
                     /locus_tag="VFB37_08335"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HZO22502.1"
                     /translation="MLLRIRAGPGDHDFCMQDRRIAVFFHGLFVLTGVLFALASSGAA
                     QARSCHGIDFPEHLKVDGSDLSLNGLGVRKATFLKVNVYVAALYVVRPSSEPGALLDS
                     KDPQELTLQFVRSVGAGDIRKGWSEGFERSAGAQLGTLQGRIARLNGWMSDVQVGQRL
                     TFIRHPGMGVQVSLNGTVMGGIEGDDFARAFVGIWLGATPPNAELKTGLLGGPCE"
     gene            8231..10159
                     /locus_tag="VFB37_08340"
     CDS             8231..10159
                     /locus_tag="VFB37_08340"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22503.1"
                     /translation="MKSVLIGAAICFAAHGAFAAEAAGPSGSTPIVPIEAPKDRAFPG
                     AIQLSVDATDTGRRIVQVHESLSGIEPDEVLLYPKWLPGTHAPEGTIDRLANLRITAN
                     GTRVAWTRDPVDVFAFRLHVKPGIRSIDIDFTYLSPTSAQVGALEMSRDLEMLEWNEL
                     VLYPAGYFARRIPVDVSLALPTGWQFGTALEQLTSNGARIAFKRVDLEHLIDSPVYAG
                     RYLARHELDPGGRAPVYLDLFADRPEFLAAKPEQLAAYRALVQQAYKLFGSHHYAHYD
                     FLYSLSDQIQQSGLEHHQSSEDGSDPESFTEWDKTAYVRDLLPHEFTHSWNGKFRRPA
                     DLWTPNYNVPMQDSLLWVYEGQTQYWGEVLAARSGLRTRQQALDSLALTAAFYQHQAG
                     RAWRALQDTTNDEIINPRRPQSWPDFQRFEDYYSEGQLIWLDVDTLIRERSQGKRSLD
                     DFARAFFGIDDGSFTIVTYTFNDIVKALNAVEPYDWASFLRERLDSVGKPAPLDGLQR
                     GGYKLVYTDTPSEFLKSSDEQRKRVSLEYSIGVLLDTKDVECDVREVQWNSPAFKANI
                     TEGSRLLAVNGIACTGDVLKDAIRTAQTGKAPIELIVKTAERYMVASLDYHDGLRYPH
                     LERDGSAPARLDEILTAR"
     gene            10320..10913
                     /locus_tag="VFB37_08345"
     CDS             10320..10913
                     /locus_tag="VFB37_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22504.1"
                     /translation="MSVLKQSIRVAAAAALLGGTITPAAAHPLLLLGPLALTHHVIGA
                     VARLASVPLVVASAALAAVPPVYAAASGYDEGPRYYPQPGPPAYPAPGYSHAPPYYAP
                     GPAYYSSAPGYRAPYYSAPGSYYPWSYSAPYYGTPYYRAPSYAGPHSYYRGSYDTRAF
                     PHYSSGYSRGYYAPHARYTAANNFHVRTASGGSHRRY"
     gene            10873..11595
                     /locus_tag="VFB37_08350"
     CDS             10873..11595
                     /locus_tag="VFB37_08350"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO22505.1"
                     /translation="MSAPPRVGPTAATETRRRAPRRKARLGGRPSSTDALRLGDRILD
                     AATELFLTEGYGSTSIEAVAARAGISKRTFYHRFENKGALFAAVVHRIIEQIRPPPDV
                     PLLEGASLHQILCRLAMFALRAALAPRAIALHRLVMAESARFPNLVRAVYDEGWAQEA
                     GELIGTLLEREVPGAPLSGPQRAFAAHQFLHMVVAFPQRRAMGLGTPMTASELEQWAE
                     DAVKLFLHGCSGLMQGRAASAR"
     gene            11712..12392
                     /locus_tag="VFB37_08355"
     CDS             11712..12392
                     /locus_tag="VFB37_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZO22506.1"
                     /translation="MCARFQTPAQAAAERYWKLIEPLWRYEQSWRVLPTDPVPIVLTL
                     NGERTGRMMRWGLIPFTGEAKFPLINATVEKLESWYAWKGPWERGQRCLFSMSGFYEP
                     HLYNSGRKEPFSVQLADRPIFGVAGLWDRSRDREGNKVLSCALITIPPNALLASVHNE
                     KQRMPAVLREEDHEAWLSGSAAEAKSTLVPYPADLMTAWQVSRKLYAIKTPNDSSLIE
                     PVPAPPDE"
     gene            12501..12845
                     /locus_tag="VFB37_08360"
     CDS             12501..12845
                     /locus_tag="VFB37_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22507.1"
                     /translation="MRSSHGTFAAVVWIVAGALLFNSEPEARLISWQTPVFFVLGMFV
                     AGAVLGGLSYIGVRGLTHALVTTRIVTAPTRRAAIGLTVTSYVIFAAQGGLIYLVAKW
                     TIRAMFQIDPGF"
     gene            complement(12851..13060)
                     /locus_tag="VFB37_08365"
     CDS             complement(12851..13060)
                     /locus_tag="VFB37_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22508.1"
                     /translation="MRTLLVLVAWLALLVLCWPVALLALFLWPIVWLLSLPFRLVGLT
                     FEALFALLRALLFLPARVLGYRSHA"
     gene            complement(13482..14021)
                     /gene="ppa"
                     /locus_tag="VFB37_08370"
     CDS             complement(13482..14021)
                     /gene="ppa"
                     /locus_tag="VFB37_08370"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZO22509.1"
                     /translation="MRLEAISIGRDPPAEVNVVVEVPVGGEPIKYEMDKAAGALIVDR
                     FLYTAMRYPGNYGFIPHTLSEDGDPCDVLVANQRGVIPGAVLAVRPVGVLKMQDEAGG
                     DEKIIAVPVPRLTRRYENVHNYTDLPEITVYQVQHFFEHYKDLESSKWVKVTGWGDAQ
                     EARTYILESIERARGKPSR"
     gene            complement(14053..14973)
                     /locus_tag="VFB37_08375"
     CDS             complement(14053..14973)
                     /locus_tag="VFB37_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO22510.1"
                     /translation="MSAQPLLGGVELGGTKCVCILGTGPDDIREHTRIPTGDCDETLA
                     QIRARFEAWRSVHGPMQAIGLASFGPLDLQPASSHYGEITSGAKPGWIGANILERLGA
                     GWGIPVGFNTDVNAAALAEGRWGGARDLSDYAYITVGTGIGVGLVVAGRPVLGCTHTE
                     LGHVRLARYPGDRWPGACPYHGDCVEGLASGPAIAARMGQSASEIPSDNPVWELVAHA
                     LSQLLQTLFLATAPRRILMGGGVIEHRPELLAQTRHLLVRGMNGYLDLEDLTGGVDRY
                     AVPAALGSLAGPAGALALAALAHGSGSWTC"
BASE COUNT         2797 a         4884 c         4883 g         2749 t
ORIGIN      
        1 gaggagcatg gcccatgagc ccgagcaccc atgtacgggg cgggtgggaa caaggtctac
       61 gtattccctg cggagagggc cgtcgtggtc atcacgacca cgaatttcca cgtttcggga
      121 gctggagcgc tcacggacaa acttctgacg gacgagattc ttccaatgct gcgcgactga
      181 gcgcgccgga agttctcagg cgcaaacgcc cactccgagc tacgagtcca agacccaatg
      241 gccgagcagg cagccccgtg gcgagtcggc ctggtgtccg ggcatctgcc gcgtcagccc
      301 tcgcataccc tgattgcgcg cagtgggaca cggtttcata atccgacccc gttcacggct
      361 ggcgcctcat catgagaaca agcagaagac atgtttgtgg gctcctcgca ttggctctca
      421 ccatcggatc cttcggcagc gcgatggctg cgggcaaacg gctggagctc gacgacgtgg
      481 cgaagatggt ccgcgtctcc gatgcacaga tttcgcccga tggccgatcc atcgtgttcg
      541 ttgtcgcgcg tccgaatatt cgcgaagcac gctatgaccg ctcactgatc ctggtggatg
      601 tggagtccgg caagcagcgc acactgactt acgaccgcag aggcgtgagc aatccccgct
      661 ggtctcccac cggcgatcgc atagcgtttc tggatcccgt ccagctcaaa cccgaggaaa
      721 aagaggacgg cgctgcgaag gccgagcagt ccccgccgga gccggcgccg ccgaacgaac
      781 cgaagtacca ggtattcgtg ctgccgatga atggcgggga agctcagcgc atcacgcact
      841 ctggcgagaa cgtcgagcag gtcgaatgga gcccggacgg gacccagatg gcctacgtga
      901 ccgcggacga gaatccgaac aggaaggaga tcgccaaaca caacgacgcc ttcgaggtgg
      961 gggacaacga ttatctggcc actgaggctg agatgccgtc gcatctgtgg ctggtgcccg
     1021 cagagggcgg tccggcgcgt cgcctgactt cgggcagctg gagtcttccc aaggcggcgc
     1081 caccgagctc gccggcctcc ccgctgtcct ggtcgcccga tggcaaatcg atcctgatcg
     1141 tgcagcaggc tacgcccaac tggggcgatt cagatcaatc cgtgattgca gcagtggacg
     1201 tagccagtgg acggatccgc aagttgactg cccacaaggc gctcgaaggg tatccgctct
     1261 attcacccga tggcgcctcg gtagcctatt ggtaccctcg cgatggtgat ccgaacaacg
     1321 gcaccgagct gttcctgacg agcgctgagg gcgggaacgg tatcgacctg acgcacgagc
     1381 tcgatcgcaa tatcgtgcgg gccatctgga atcgcggtgg cgacgctctt ttggtggcgg
     1441 cacatgaccg ggtgcgcgtg ggattgtggt accagccacg caagggcaat gcgaagcggc
     1501 ttgatctggg cgacgtcgat cccagctgga gtttctgggc cgatgtgact ttgggaaaga
     1561 atggggcgct cgccctggcg ggcagcacgc catctcatcc caacgagatc tactacgtac
     1621 cgccgggcgg cggcgcgccg aagtgtctga cgagcttcaa ccaggaaatt gccgcacgcg
     1681 agatcggccg cgccgaggag ctcacctgga agggggccga cggagtgacg gaggacggtg
     1741 tggtcgtcta tccgccggag ttcacgccgg cgcacaaata cccgctggtg ctggtcatcc
     1801 atggcggacc ccaggcttct tcggcgcttt cgttcagtgc tctctcccaa ttgctcgcgg
     1861 cgcgcgggta cgtcgtcttt tcaccgaact accgtggcag tgacaacctg ggcaacgcct
     1921 acgagcatgc gatcttcaac gatgccggcg cgggcccagg gcgggacgtc atggccggac
     1981 tgtcggcgct cgaggcgaag ggattcgtag atacgagccg catcgcggtc tccggttggt
     2041 cctacggcgg atacatgacc tcgtggatga tcgggcacta ccacatctgg aaggcggccg
     2101 tctccggcgc agcggtcaac gatctcattc acgagtatgc cctggcagat ttcaacgtga
     2161 cggagcgcta catctttggc ggccctccgt ggtccgagaa gctgctgcag gcgtatcggg
     2221 agcagtcgcc gatctcttac gcctggcaga tcaccacacc gaccctgatc ctgtcggata
     2281 ccggtgatgc gcgtgtgccg atctcccagt cctatctcat gtatcacgcc ctcagggacc
     2341 acggagtgac cgtgcagttt tatgcctatc cggttccggg ccatttcccc ggtgacccgg
     2401 tgcgctccac cgatgtctat cggcgctgga tcgaatggat agacagatac ctgcgcaagc
     2461 cctgaggggc tccgcgactc gcgtgcaaac cccaccccga ccccacgacc gcgccccacc
     2521 ggatccctcg gcccgccagg tgcgcgaggt gccaggggct caatcgcgcc aggccgcgtc
     2581 ctgcaaaagc tctcgacgag ctgccgttgc aggcgttggg gcaagccttt tgactctggc
     2641 cgggcagcgc ctgtatccgc agaatgcgct cggggccgaa acctcgacgg gatctgccgc
     2701 gcagccgaac gcctcagcgg ccggcaccct ccgcagcggc gatctgaccg tcaatcgcat
     2761 cggatatggg gcaatgcgca tcaccgggga tggaatctgg ggtgcgcccg ccgatgttcc
     2821 ggaggcgcgt gcggtgcttc gccgagccat cgagctcggg gtaaacctga tcgataccgc
     2881 cgatgcgtac gggcccgacg tgagtgagca gctcatttac gagtccctgc atccgtatcc
     2941 ggcgggtctg gtgatcgcga ccaagggagg ctttacgcgc ccgggaccgg atcaatgggt
     3001 ggcggacgga aggcccgagc atctacgtgc ggcatgcgag ggcagcctca ggcgcctgca
     3061 cctggagcgc atcgatctgt atcagcttca taccccggat ccgaacgtcc ctttcgagga
     3121 ttcaatcggg gagctcgtgc gcctgcgaga gcaggggaag atccgctaca tcggagtctc
     3181 caacgtgacg ctggcgcagc tcaagcgcgc tcaggccctc gcgccgatcg tctcggtgca
     3241 aaatcgatat aacgtcggcg atcggggtaa tgaggcagtg ctgcaatact gcgcctcgca
     3301 gcgcctggcc tacctgccgt gggcaccgct cgggggtcgc aggtcgcggc gccgcaggat
     3361 tttggaggct ttgtggtctt cctccgccga gcgcgttgca caggcgcact cggtgagtac
     3421 cgcgcgcgtg gtcctggcgt ggctgctgca gcactcgagc gcgctgctgc cgatcccggg
     3481 aacctcttcc cgggcgcatc tggaggacaa tgtcgcagcc ggcaccctgc gtttgacgcc
     3541 ggcagagctg gccgagctcc aaagctgagg aggacctcga tgcgcccgac ccactacgag
     3601 catatcgcca cgaacggctt cacaaccgtg actcgctcag tgggactgat tgtcctggtg
     3661 ggctggtgcg gattcgccct cgcccagaca agcccagcca agcaaaccga ggcgcagcag
     3721 cgagtgcagc agaagatcct gcagcagaag aagctggaat gcgccaagct gggaaaatat
     3781 gccgtcgcca gcacgattcg ggggaccaca ctgtacaagt gcgccgcgcg cccgaacggg
     3841 tgagggcgac caaaagcctc gcgcagcagt tcctcagaac gccacgagtc aggacgcgcg
     3901 aggggcctcg cccccagctc cgtccggtgg cgcctgcgtc gccgctgaat tctcgcgctg
     3961 aatccgcacg aagatctcct cacgatggac ggcaacggat ttcggcgcat tgatgccgac
     4021 gcgcacctga ttgcctttca ccccgagcac ggtgacggtg acgtcgttgc ccaccatgag
     4081 ggtttccccc actcttcgcg tcagtatgag catggcggcc gactcctgcg tttccgcgtc
     4141 cgggctggag agcctacgcc aactcggtcg cggtcgcccg aaatttcgcg gcagcgcccc
     4201 tggagcagtc tgcgcagcgt ggattgcgat gcaccaggga gcgctcaggt cccagttccg
     4261 ggtggaccct cgaggcactt tctgcaatcc aacttttccc cgagcgctgc cgcgcgcccc
     4321 tgtgcagtcg tgacccaggg ccgattcacc caaggcgggt catgacgcac gtgttggccg
     4381 tgaccacact cgagctccgc cacccaatga ccttcgtcgt cctggtggta gccgatgatc
     4441 tttctttgca cgtgaggagc gctggccagc acgtagaaaa agccggcggc ggcgcgaagc
     4501 gcacgccgcc ggctaggcgg ttagagcagt tcccggaacc tgcgatcagt acacagccag
     4561 ggcattcgag cccgcaccgt gtccgtccgg ggcactcacg tagctgttga ccagcgtcag
     4621 cgccccggtg gcgccgatcg tgaacagcga aaccgagccg tcaatcttgt tcacgcagta
     4681 caggaactgg ctcgagggat cgatcttgat atcgatcggc cccgggttgg ccaccgcagt
     4741 cacattgccc gtggccaccg ccgacccgat ggccgtcagc gcacccgtct gcgcgttgat
     4801 cgagaaggcg gacagagtac cgtccgtata gttggtgaca tagagatatt tgccggtggc
     4861 gtcgatggca atgcccgccg tgccattgcc ggcaggggcg ctacccacca gtgtcacatt
     4921 tccggtcgag tcgtaggaga ataccccgac gttgttcgaa aggttgttgc tcgtatagac
     4981 gtaccctcca ctcgagccgc ccttgggcca cacggcgaca ccgttgattc cctccaagct
     5041 cgcgtccgag gcgcccgatc cgaccgtctg cgtgagcgtg ccatccgctg cgatggcgtt
     5101 gacgtagagg aagtgcggat cgccggcact gaacaccagc gagctcgtcg gatcgatcgc
     5161 gatgccgagc tgcgggttgc tgaaggggaa gggcgcgttc ggattcacga ctccgatgag
     5221 cgcacccgaa agcgggttga cactgaagcc cagaatgacg ccagggtttg aggtgccatg
     5281 ccccgaggcg tacaggaacg tatcggcagg ggtaatcgcc agcgccaccg gcaccgtgga
     5341 tgtcgtgctg aactgccctt gcagcgtcag actgccgtcc gaattgagca tctcgcggga
     5401 aacatcgtcg gagcctccat tgctcacgta aacgaattgt ccggacggat caacgatgac
     5461 tccgttggga cccacgtccg cggggaacag ggtgggcgtg ccgaccccac ccaccggggt
     5521 cagggcgccc gtctgtgcat tgatcgtgaa cgaggttata tcaccctgtg cgccatctgc
     5581 gttgttggcc acaaacacgt actggccgac tctcgcgcag gcgacctgta cgttggtcac
     5641 actggcgccg gcgattttgc cggatccatt cgtgaccgtg cagcgctgca ccggcgcagt
     5701 gggttgtgtc aggaccgtca cagcgtaggc cgccccgttc agcagggtgc ccgtgaacgt
     5761 gaagttcgcg gagctgccgg tgggtgcggc gacagctagg ttattccctc cgttgtcctg
     5821 aagcaccagg ccgctgcccg tgagcccggt gaccgtgccg ctgaccgaat aggcggtggt
     5881 cgtgcaggcg actgcgaccg tggtgacgtt tgcgccagcc accgtgccgc tcccattagt
     5941 caccgtgcac ttctggccgg gtggagaagc agagacggtg accttgtaag cggctccgtt
     6001 ggtcagcgcc tggctgaatg tgaacttggt ggcgcctgat gcgacggtga gattgttgcc
     6061 gccgttgtcc tgcagaacca ggccattgcc cgagagaccg gagatcgtgc ctccgacggt
     6121 aaaggtggtc gtcgcggagg tgcaggagac ggcgacgttc gttacgttcg cgccgctgac
     6181 cgtgccgctg ccgttactga cggcgcactt ctggccgctt ggagaggtca tcaccgtgac
     6241 agcgtacttc gagccactgg cgagcgccgt ggcgaacgtg aaactcgtgc tgcccgaggc
     6301 aacggtgaga ttgtctccgc cattgtcctg gaggacgagt ccgcttccat tgagaccgct
     6361 gatgctgccg ccgatggtat aggtctgcgg cggtggcgga ggaggggttg tggtgctcga
     6421 tgagccgccg ccccctccgc atgcgctggc cagaaggccg atggatagtg cagatgcggc
     6481 cgcgcgcacc catccgaggt gaagtcgccc tgattgtgtg agtgacatac tggtcccctg
     6541 tagtcgatct tgttgccgcg cgctgctctg ataagcggat cgtcagcaaa ggctgacggg
     6601 gtcgatctgt gacgcgtgca ccgattatta gtgatgcttt gcgggaaaaa ctgtgagcca
     6661 aatcacatga gacctcctgt tgttagaagc acacaaggga cagccgcttg gggtttgact
     6721 taaacgttca agctgcgagt cgaggctaca tatgccgacg cacatggtca aaaggtaact
     6781 cgttgccttt cgtccctgaa aggcgatcgc tcatctggcg ctagcgttcc ttccagtcac
     6841 cgaaatgcat cggatcttca ttcttgtgcg agaaaagatc gtcacgcggc tcgatcttgc
     6901 cgatccgaca ggcaaatccc ggaatgacaa tgaaaattcc cacgacacag tgctcattga
     6961 gatgccacag cagtgcgccg tgcgtgatct ccatgccgcg ggagcgctcg gaccacccga
     7021 cccaaggccg cctgaagcgg cgggaactcc agcccgatgg cgcttgagtc ggggcgcgca
     7081 gtgcgccggc ttttcgggcc gtcgcgtcct cggattcgat ttgccgttgg gtggcatcgt
     7141 ccgcctcttt ctgcgcttca tccatccagt cgatacgagc cgcggactcc ggtgcaggcg
     7201 cgagcgcagg ctcggcagct gcagcagcac gcaccggggg cgcgcgcgag cgttcgggga
     7261 ccttctgcgc ttgagaaggg ggacggggca ggtcgagcgg cggctccaga agggtcagtg
     7321 ccgtcgcctc gagctccgac tcttcgagcg ttgcttcgga gtggactcgc agacagaagt
     7381 agtagatcag tgtgccgtgg gcagcggcga ctgcgatcag tgcagtgaga cgcgatatgc
     7441 gcaaccgggc gctccctgtc gagctccgcg cgagctgcgt agtgcgactg actcggcccg
     7501 ggagaagttc ccggcgcgat catctcattc gcacggacct ccaagcagtc cagtcttcag
     7561 ttccgcgttg ggcggcgttg cgcccagcca gatcccaaca aacgctcggg caaaatcgtc
     7621 cccttcgatg cctcccatca ccgtgccgtt gaggctcacc tgaacgccca tgcctggatg
     7681 gcgaatgaac gtgaggcgtt ggccaacctg cacatcggac atccagccgt tgagtctcgc
     7741 gatgcgcccc tggagtgtgc ccagctgagc tccggcactg cgctcgaatc cctcgctcca
     7801 gcccttgcgg atatcgcccg cgcccacgga ccgaacgaac tgcagggtga gctcctgagg
     7861 atccttcgag tccagcaacg caccgggctc ggaggagggc cggaccacgt agagtgccgc
     7921 gacgtagaca ttcaccttca agaaagtggc cttgcgcacg cccaaaccgt tgaggctcag
     7981 gtcggagcca tcaaccttaa ggtgttctgg gaagtcgatg ccgtggcacg agcgcgcctg
     8041 agcggcgccg ctgctcgcca gcgcgaatag gactcccgtc aatacgaaga gcccgtggaa
     8101 gaacaccgcg atacgtcgat cctgcatgca gaagtcatga tcgcccggcc cggcacggat
     8161 gcgcaagagc acccatgcta cgataggagt tccactgcgc cctcaagaac acaaccgcca
     8221 gggatcaatc atgaagtccg ttctgatcgg tgcagcgatc tgcttcgccg cgcatggggc
     8281 tttcgctgcc gaggccgcag gcccgagcgg gagtacgccg atagtaccca tcgaagctcc
     8341 caaggaccgc gctttccccg gcgcgatcca gctcagcgtc gatgcgaccg ataccggacg
     8401 gcgcatcgtc caggtgcacg agagcctgag tggcatcgaa cctgacgagg tgctcctgta
     8461 tccgaagtgg ctcccgggca cgcatgcgcc cgaaggcacg atcgatcggc tggccaacct
     8521 gcggatcacg gcgaacggca cacgagtggc ctggacgcgc gaccccgttg atgtctttgc
     8581 gttccgcctg catgtcaagc cggggatccg atcgatcgat atcgacttca cctacctctc
     8641 gccgaccagc gcccaggtgg gcgcgctgga gatgagccgc gacctggaga tgctcgagtg
     8701 gaatgagctc gtgttgtacc cggcgggata tttcgctcgg cgaattcctg tcgatgtgag
     8761 ccttgcgttg ccaaccgggt ggcagttcgg aaccgcattg gagcagctga cgagcaatgg
     8821 tgcgcgcatt gcattcaagc gggtcgatct ggagcatctg atcgacagtc cggtgtacgc
     8881 gggccgctac ttggcccggc acgagctcga cccaggcggt cgcgcaccgg tgtatctgga
     8941 tctgttcgcc gaccggccgg agtttctagc tgcgaagccc gagcagctcg cggcttaccg
     9001 cgcgctcgtg cagcaggcct acaaactgtt cggctcccac cattacgcgc actacgactt
     9061 cctctactcg ctcagcgatc agattcagca aagtgggctc gagcaccacc agtcgagcga
     9121 agacggcagc gatcccgagt cgttcaccga gtgggacaag acggcctacg tccgcgacct
     9181 gctgccgcac gagttcactc actcgtggaa tggcaaattc cggcgaccag cggatctgtg
     9241 gacgcctaac tacaacgtgc cgatgcagga cagtctgctc tgggtctatg aaggtcagac
     9301 gcagtactgg ggcgaagttc tggccgcacg ctcgggactg cgcacccgcc agcaggccct
     9361 cgattcactt gcactcactg cagccttcta tcaacaccaa gcggggcgcg cgtggcgagc
     9421 gctgcaggac acgacgaacg atgagatcat caatccgcgt cggccgcagt cctggccgga
     9481 cttccaacgt ttcgaggact actacagcga gggccagctc atctggctgg atgtggatac
     9541 gctgattcgc gagcgctcgc agggtaagcg ttcgctggat gacttcgcac gggcattctt
     9601 cggaatcgac gacggcagct tcacgatcgt cacctataca ttcaatgaca tcgtcaaggc
     9661 gttgaacgcg gtcgagccgt atgactgggc ctcgtttctg cgtgagcggc tcgactcggt
     9721 gggtaagccg gcgcctctgg acggattgca gcggggtggc tacaagctcg tctacaccga
     9781 taccccgagt gagtttctga agtcatcgga tgagcagcgc aagcgcgtga gcctggagta
     9841 ctcgatcggc gttctgctcg acaccaagga tgtcgagtgt gacgtacggg aggtccagtg
     9901 gaacagtccg gctttcaagg ccaacatcac ggagggttcg cggctgctcg cagtgaatgg
     9961 catcgcctgc actggggatg ttctcaagga tgccatccgg acggcacaga ccggcaaggc
    10021 ccccatcgag ctgatcgtga agactgccga gcgctacatg gtcgcgagcc tcgactatca
    10081 cgacggcctc cgatacccgc atctggagcg ggacggctcc gcgccggcgc gcctggatga
    10141 gatcctgacg gcgcgctagc cagctgcggc tactgatacg cggcgactgc cgccacgggt
    10201 ccggcggtcg cggtctgcag gcgccgatcg tgaaacgcaa cggtaccgtt gcgtttccat
    10261 ccgcggacgc gtaccgtcct gttcctaggg cctggggaag aggctgaaag ggggtactca
    10321 tgagcgtgct caagcaatcc attcgggtcg cggccgcggc cgcgctactc ggcggcacca
    10381 tcactccggc tgccgcacat cctttgctgc tcctgggtcc gctcgccttg acgcatcatg
    10441 taattggcgc cgtggcgcga ctcgcgagcg tgccgctcgt cgtggcctct gcggccctcg
    10501 cggcggttcc tcccgtctac gcggcggctt cgggctacga tgaaggaccg cggtactacc
    10561 cgcagcctgg acccccagcc tacccagcgc cgggctactc ccacgcaccg ccgtactacg
    10621 cacctggacc ggcttactat tcaagcgcgc ccgggtatcg cgccccgtac tactccgcac
    10681 cgggttcgta ctacccgtgg tcttattctg caccttacta cgggacgcct tactaccgag
    10741 cgccttccta cgcggggccg cacagctatt accgcggcag ctatgacact cgcgcgttcc
    10801 cgcattactc ctcgggctac tcacgcggtt actatgcccc gcacgcgcgc tacactgcgg
    10861 ccaacaattt ccatgtccgc accgcctcgg gtgggtccca ccgccgctac tgaaactcgt
    10921 cgtcgcgcgc cgaggcgcaa agcgcgcctg gggggcagac cttcgagcac ggatgcgctg
    10981 cggctggggg atcggatcct cgatgccgcc accgagctct ttctgaccga gggctacggg
    11041 tcaacgagca tcgaggccgt ggctgctcgt gcgggcatct ccaagcggac cttctatcat
    11101 cgattcgaga acaagggcgc gctgttcgca gccgtggttc ataggatcat cgagcagatc
    11161 cgcccgccgc cggacgtgcc cttgctcgaa ggcgcgagtc tgcatcagat cctgtgccgc
    11221 ctcgcaatgt ttgcgctgcg cgcggcactc gctccgcgtg ccattgcgct gcatcgactc
    11281 gtcatggcgg agtcggcgcg ctttcccaat ctggtacgtg ccgtctatga cgagggctgg
    11341 gcgcaggagg cgggcgagct gatcggcact ctgctcgaac gcgaggttcc aggcgcgccg
    11401 ctcagcggcc cccagcgagc cttcgcggcg catcagtttc tgcacatggt cgtggccttt
    11461 ccgcagcggc gcgccatggg gctgggcacg cccatgactg cgagcgagct cgagcaatgg
    11521 gccgaggatg ccgtgaaatt atttctgcac ggctgcagcg ggctcatgca gggtcgggcg
    11581 gcttccgcgc gctgaagaaa ctctcgaccc gctgagcggc gtcgggcgcc agccctcgat
    11641 cctgccaggg gataggatag aagcggagga atcagccgtg acgaagtatc tctagccgct
    11701 gcagaacgcg catgtgtgcc cgatttcaga ctcctgcgca ggccgccgcc gagcgctact
    11761 ggaaactcat cgagccgcta tggcggtacg agcagagctg gcgcgtgttg ccgaccgatc
    11821 cggttcccat cgtgctcacc ctcaatggcg agcgcaccgg gcgaatgatg cgctggggac
    11881 tcatcccgtt taccggggaa gcgaaatttc ctctcatcaa tgccactgtc gagaagctcg
    11941 agagctggta tgcctggaaa ggcccatggg agcgcggcca gcgctgcctg ttctccatgt
    12001 cgggcttcta cgagccacat ctttataaca gtgggcgcaa ggagcccttc tctgtgcagc
    12061 tcgcggatcg gcccatattc ggagtcgccg gactgtggga tcgttcgcgc gatcgtgagg
    12121 gaaacaaggt cctctcctgc gcgctcatca ccattccgcc caatgcgctg ctggcctcgg
    12181 tgcacaatga gaaacagcgc atgccggcag tgctgcgcga ggaagatcac gaggcctggc
    12241 tgagcggcag cgctgcggag gcgaagagca cgctggtgcc gtatccggcg gatctcatga
    12301 ctgcctggca ggtgagccga aagctttacg ccatcaagac accgaacgat tcgagcctga
    12361 tcgagccggt tcccgcgcct ccggatgagt gaggcctcga gcttcagcag ccgttgccgt
    12421 gcgcgcagtg ggccttgcca acccgagtgc gaatcggctt acactcgcaa gcttccggca
    12481 agcggtgcag gcagcatcag atgagatcga gccatggcac attcgcggcg gtggtgtgga
    12541 tcgtcgccgg tgcgctgctg ttcaattcgg agcccgaggc gaggctgatt tcctggcaga
    12601 cgccggtctt cttcgtactc ggcatgttcg tcgccggtgc ggtcctcgga ggcttgtcct
    12661 acattggcgt gcgcggcctg acgcacgcgc tggtgacgac ccgaatcgtg acggcgccga
    12721 cccggcgggc ggcaatcggt ctcacggtga cgagctacgt gatcttcgcc gcgcagggcg
    12781 gactcattta tctcgtggcc aagtggacga tccgggcgat gttccagatc gatcctggtt
    12841 tctgatcgcc tcaggcgtgg ctgcggtagc ccagcacccg ggcaggcagg aacagcaacg
    12901 cgcgcagcag cgcaaaaagc gcctcgaagg tcaggccgac cagacggaag ggcaacgaga
    12961 gcagccacac gatcggccat aggaaaagtg ccagcaacgc cactggccag cacagcacca
    13021 gcagggcgag ccaggccacc aagaccagca aagttctcat tacacgcctc acgccgcccg
    13081 aaacgttctg cattatctca ccgcgcggca gctgtagcca aagcggcggc tcgcatcggc
    13141 tgcacggttc accgccgcgg atacccggcc cttcgcagcc ccaagcgacc gccggcccgc
    13201 acgaggccgg cggtcagatc gcttacctcg atggcaggaa gcggttgcgc acgacgacgg
    13261 ccaaatggcc gatgagcagt gcggcggtgg cgatgatttc gatgagtagg atggtcatgg
    13321 gaaggccctc cggtctgtca gcgccgcgca gtttacggat gcctcggctg ggcgatagag
    13381 accttgcgag ggacgcgctc gggtattcgg ggggattgca atttcgctgc ctggccccca
    13441 caaaagcccg aagggcgggc acttgagcgc tcttagtggc gctacctcga gggcttgccg
    13501 cgggcccgct cgatggactc gaggatataa gtccgggcct cctgagcgtc tccccagcct
    13561 gtgaccttca cccacttgga gctttccagg tccttgtagt gctcgaagaa gtgctgcacc
    13621 tgatacaccg tgatctccgg cagatccgtg tagttgtgca cgttctcgta gcgacgcgtg
    13681 aggcgaggca cggggactgc gatgatcttt tcatctcccc cggcttcgtc ctgcatcttg
    13741 agcacgccga cgggacgcac tgcgagtacc gctccgggga tcaccccgcg ctgattggcc
    13801 acgagaacgt cgcagggatc gccgtcctcc gagagagtgt ggggaatgaa gccgtaattg
    13861 ccggggtagc gcatcgccgt atagaggaaa cggtcgacga tcagcgctcc ggcggccttg
    13921 tccatctcat atttgatggg ctccccaccg acgggcactt ccaccactac gttgacttct
    13981 gcaggcggat cacgcccgat cgagatcgct tccaggcgca tctggcttcc tcgaggtgaa
    14041 cggttgcgga gcttaacagg tccagctccc ggagccgtgc gccagcgccg caagagcgag
    14101 tgcgcccgcg ggcccggcga gcgaacccag tgcagcaggc acggcatatc gatccacgcc
    14161 gcccgtcaga tcctccagat cgaggtagcc attcattcct cgcaccagca ggtgccgcgt
    14221 ttgcgcgagg agctcaggac gatgctcgat cacacctcca cccatgagga ttcggcgggg
    14281 tgctgtcgca aggaacagcg tctgaagaag ttgagacagc gcgtgagcaa ccagctccca
    14341 cacaggatta tctgaaggaa tttcgctcgc agactggccc attcgggcgg cgattgccgg
    14401 gcccgaggcg agcccttcta cgcagtcgcc atggtacgga cacgcacccg gccagcggtc
    14461 acccggatac cgcgcaaggc gtacatggcc gagctcagtg tgcgtgcaac ccaacaccgg
    14521 gcggcctgcc acgacgaggc cgacgccgat gccggtgcca accgtgatgt acgcatagtc
    14581 ggacaggtca cgtgctcctc cccagcgacc ttccgccagc gctgcggcgt tgacatccgt
    14641 gttgaatccg acggggatgc cccatccggc gcccaatcgc tccaggatgt tcgctcctat
    14701 ccatccgggc ttcgcacccg aggtgatctc cccgtagtgc gacgaagcgg gctgcaagtc
    14761 gagcggcccg aatgaggcca gtccgatcgc ctgcatgggc ccatgcacgg aacgccaagc
    14821 ctcgaacctg gcgcggatct gcgcgagcgt ctcatcgcag tcccccgtgg ggatgcgcgt
    14881 gtgctcgcgg atgtcatccg ggcccgtccc caggatgcac acacatttgg tgccgcccag
    14941 ctcgacgccg ccgagcagtg gctgcgcgct cattccggca ctcctgtggg catttgactt
    15001 aattaaaatc ataggctctt cagcgggcac accaaagtgt cggctggcac agacggtgca
    15061 agtgatcgtg ctggcgtcca ccgagcgccg ggcgcacact ccgcgggcac cacattatgt
    15121 cgttgccagc cgaggggcat cccctcacgg gcgggagtca tgcgcattca gggcgtcacg
    15181 cacgcgcgtc tcatggacga gtcggaaatc tgtatcgccg atacggcgaa attcggcacg
    15241 cccgcgcaga tgctcgtcgc gagccgcctg ctggaacacc cggagactta tcgacgctgg
    15301 gaatcagagc acg
//
LOCUS       DASRHN010000217         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1058690, whole genome shotgun sequence.
ACCESSION   DASRHN010000217 DASRHN010000000
VERSION     DASRHN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058690"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFB37_08380"
     CDS             complement(<1..205)
                     /locus_tag="VFB37_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22511.1"
                     /translation="MQPISALAGNSAANPGRSGAARYKPGVLKYAQMGYWDADYEPRE
                     SDILALFRITPQEGVDPIEAAAAV"
     gene            372..1319
                     /locus_tag="VFB37_08385"
     CDS             372..1319
                     /locus_tag="VFB37_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZO22512.1"
                     /translation="MRNVTLRQMKVFAVVARHLSFTRAARELHLTQPAVSQQIRLLEQ
                     EVGLPLFEKIGRKVHLAPAGEELLRYANQTLELLREASESLAAMRGLRRGVLKLGAVS
                     TAKYFAPSLLSAFTPAYPEVTIKFAVGNRGEIIKQLAANEIDLVIMGRPPRELDTIAE
                     PFAKHPLIIIASPGHPLARRRRIALQQLASQQFLIREEGSGTRASMEYVFRERQVPFR
                     VSMEVSSNETIKQAVMANMGISFISAHTVGLELAAGKLASLNVIGLPIVRDWYVIHLR
                     EKHLAPIAAAFRAFLLSQGGRIIHQAVGDLPRVRRRAKT"
     gene            1409..2233
                     /locus_tag="VFB37_08390"
     CDS             1409..2233
                     /locus_tag="VFB37_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22513.1"
                     /translation="MRVELDDSAFPVGATPLMSWIGRSLQIVAAYYGRFPTDTLRIRV
                     IPVAGTGVRNGKTFGYRGGSIRVDVGRDVTEPQLLNDWVLVHEMTHLALPDVGEDHAW
                     LSEGLAVYVEGIAREQAGNRTQQDVFAEELRSMPRGLPQPGDEGLDHTHTWARTYWGG
                     AMFCLLADVEIHQRTNNRLGLQDAMRAVAKASGGLSSDWPIERVMATGDAATGTTVLA
                     DLYARMKDAPFDPDLAALWSKLGVVAQGGTVELRDDAPLAAVRRAIMQPRVNKTSS"
     gene            complement(2199..3056)
                     /locus_tag="VFB37_08395"
     CDS             complement(2199..3056)
                     /locus_tag="VFB37_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZO22514.1"
                     /translation="MWTLPDILSVICRAFSFVLLLQAAGTALFIAAFGRRATDSLAGI
                     RSFGCLSAIGALLFVVAHYILEPARMAGDMSGVLDPAMQALALQSPTAEAFGTRLLGL
                     VVIALGLRLARPIVGVVGACLAALAFTLVGHTVVSPHRALLAPLLLVHLLIAAFWIGS
                     LGSLYIASIREAPAVSARLTAAFSAMAIWLVPCIFLAGVSMTALLVPGLRVFLQPYGE
                     LLLAKTTLFALLLGLAALNRWKLGPRLATDSRAVDGFRRTVAIEYVLVCSVLTVTAVM
                     TMFYSPEAA"
     gene            complement(3097..3465)
                     /locus_tag="VFB37_08400"
     CDS             complement(3097..3465)
                     /locus_tag="VFB37_08400"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HZO22515.1"
                     /translation="MKIQAGVALSALLAALVLLAWSSGARAHTHLEKSVPANGAVLNA
                     APENIVLTFAEPARLTALSLRKNSEPAQKLAPLPSATARELTVALPRLTPGKYVVSWR
                     VLSEDGHVMPGDLSFTIAAQ"
     gene            complement(3491..4255)
                     /locus_tag="VFB37_08405"
     CDS             complement(3491..4255)
                     /locus_tag="VFB37_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HZO22516.1"
                     /translation="MSPRGVRAAPAALTAMLAMHTASPLPQQMSFQDMAATMQMDDTA
                     RFGGVRVDQLEWRSAPTGEPDAAWDAEAWYGGDYDKLWVKTEGGYAAGSGGLRNTSVD
                     LLWNRVVSPWWNVQAGLRQDLAQGQSRSWVALGLQGLAPQWVETEVTLYASDEGRTAA
                     RLKVQYELLLTQRLILEPFAEANLYGRPDPQRQMGSGLAEAELSARLRFEVRRDIGPY
                     VGLVWLRNFGGTAERLRAAGASAGAVEWTLGLHAAF"
     gene            complement(4252..>4864)
                     /locus_tag="VFB37_08410"
     CDS             complement(4252..>4864)
                     /locus_tag="VFB37_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZO22517.1"
                     /translation="RGTLAPVAGMQAQIPPLDPRPLLSMADMAMGNADQAPAHFGPNV
                     DAVAIQPASRLDDPGVGLRNNGRRVLSYADLHTLDGPVDGRQAGREIRLHLTGHMQRY
                     VWSFDGRKFSESVPLLFRNAERLRIVLVNDSMMAHPIHLHGMWSEVQSADGAFLVRKH
                     TTAVQPGQELQYEVTAGALGHWAFHCHLLYHMEAGMFREVIVA"
BASE COUNT          889 a         1612 c         1562 g          801 t
ORIGIN      
        1 ctaccgcagc ggctgcctcg atcggatcga caccctcctg cggcgtgatc cgaaagaggg
       61 ccaggatgtc gctctcgcgc ggctcgtagt ccgcgtccca gtaacccatc tgggcgtact
      121 tcaagacacc gggcttgtat cgcgcggcac ccgagcggcc cggatttgcc gcgctgttgc
      181 cggcgagtgc gctgatgggt tgcatcgtat tcctccgctt cgtgagtgcg ccctgcggct
      241 ctggggcggc acggcatcga tgcatgccat cctggcccgg ccttcgcata agatccagtt
      301 agatattatt ggtacagcca taagctttct cttatcattc gcagcttccc tggctgccgg
      361 ccttccctcg catgcgcaat gtcaccctgc ggcagatgaa ggtgtttgcc gtcgtggcgc
      421 ggcacctgtc cttcacgcgc gcagcgcgcg agttgcacct gacacaacct gccgtgtcac
      481 agcagatcag gctgctcgag caggaagtcg gcctgccgct gttcgaaaag atcggccgca
      541 aggtgcatct ggcgcctgcc ggagaggagc tgctgcgcta tgccaatcag actctcgagc
      601 tgctgcgcga agcgagtgag tcgctcgccg ccatgcgcgg gctacgccgc ggagtgctca
      661 agctcggcgc agtgagcacg gccaaatact tcgcgccctc gctgctgtcg gccttcacgc
      721 cggcctatcc cgaggtcacc atcaaatttg cagtcgggaa tcgcggcgaa atcatcaagc
      781 agctggcggc gaatgagatc gacctcgtga tcatgggccg gccgccgcgc gagctcgata
      841 ccatcgccga gccattcgcg aagcatccgc tcatcatcat cgcctcgccg ggacatccgc
      901 tcgcccgccg ccggcggatc gcgctacagc agctcgcctc gcagcagttc ctgattcgcg
      961 aagaaggctc cggcacgcgc gcgtccatgg aatacgtgtt tcgggaacgc caggtcccgt
     1021 tccgcgtctc catggaggtg agcagcaacg agaccatcaa gcaggcggtc atggcgaaca
     1081 tggggatcag cttcatctcg gcgcataccg tcgggctcga gcttgccgcg ggtaagctcg
     1141 ccagcctgaa tgtcatcggc ctgcccatcg tgcgagactg gtacgtgatt catcttcgcg
     1201 agaagcacct ggccccgatc gccgcggcgt tccgggcctt tctgctgtca cagggcggac
     1261 ggatcatcca tcaggccgta ggcgatctgc cgcgcgtgcg gcggagagcg aaaacatgaa
     1321 gggtctcatc gcctgcgtcc tgttatgcgc cgcgtgcgcg tgcgccgacg gccagcagcc
     1381 ccgccagctc acgctacaag gagttgacgt gcgcgtagaa ctggatgact cggctttccc
     1441 ggtgggagcg acgcccctca tgagctggat cgggcgctcg ctgcagatcg tggccgccta
     1501 ttacgggcgc ttcccgactg acaccctgcg gattcgggtt atccccgttg cgggcactgg
     1561 cgtgcgcaat ggcaagacat tcggctatcg cggcggctcg atacgggtgg acgtgggtcg
     1621 cgatgtcacg gagccccaac tgttgaacga ctgggtgctg gtccacgaga tgacccatct
     1681 cgctttgccc gatgtaggcg aggaccacgc ctggctctcg gaaggcctgg ccgtgtatgt
     1741 cgagggcatc gcgcgcgaac aggccggcaa tcgcacgcag caggacgtgt tcgcagaaga
     1801 gctgcgctcg atgccgcgcg gactgcccca accgggcgat gaaggtctgg atcacactca
     1861 cacctgggcg cgcacgtact ggggcggggc catgttctgc ctgctcgccg acgtcgagat
     1921 ccaccagcgc accaacaatc ggctcggact tcaggatgcg atgcgggcgg tcgccaaggc
     1981 gagcggcggt ctgagctcgg actggccgat agaacgggtg atggccacgg gcgatgccgc
     2041 caccggaacg accgtgctcg cggacctcta tgcgcggatg aaggacgcgc cgttcgatcc
     2101 ggatctggcg gcgctgtgga gcaaactcgg ggtggtcgcg caaggtggga cggtcgagct
     2161 gcgcgacgat gcgccgctgg cggcggttcg ccgcgccatc atgcagcctc gggtgaataa
     2221 aacatcgtca tgaccgcggt caccgtgagg acgctgcaca ccagcacgta ctcgatggcc
     2281 accgtgcgcc ggaatccgtc cacggccctc gaatccgtcg cgagccgcgg gccgagcttc
     2341 caccgattca aggcggcgag gcccaggagc agcgcgaaca gtgtcgtctt ggcgagcagc
     2401 aactcaccat agggctgcag aaacacgcgc aagccgggca cgagcagcgc cgtcatgctc
     2461 accccagcga gaaagataca ggggaccagc cagatggcca tggcagaaaa ggcagctgtg
     2521 aggcgggcgg acaccgccgg agcctcgcgg atgctcgcga tgtagagcga cccaagagag
     2581 ccgatccaga acgctgcaat caacaggtgt acgaggagta agggggcgag cagtgcccga
     2641 tgaggactca cgaccgtgtg acccacgagc gtgaaggcca gcgccgcaag gcaagccccc
     2701 actacaccca cgatcggtcg agcgagacgc agtcccagcg cgatcaccac gagccccagc
     2761 agccgggtgc cgaaggcctc cgcggtcggc gattgcagag cgagcgcctg catcgccgga
     2821 tccagcactc cgctcatgtc accggccatg cgcgcaggct cgaggatata gtgcgccacg
     2881 acgaacagca gagcgccgat ggcggacaga cagccgaagc tgcggatgcc ggcgagactg
     2941 tccgtcgccc gccgcccgaa tgcggcgatg aacaacgcgg tgccggcagc ctgcagcagc
     3001 aggacgaacg agaaggcccg gcagatcacc gagaggatat ctggcagagt ccacacggaa
     3061 gtcgtgcaga gatcccgagc tcggaggcgt tgctcgctac tgagcggcga tcgtgaaaga
     3121 cagatcgcct ggcatcacgt gcccatcctc gctgaggacg cgccagctca ctacatactt
     3181 acccggcgtc agacgcggaa gcgcgaccgt cagctcgcga gcggttgcgc tgggcaacgg
     3241 agcgagcttc tgggccggct cactgttctt gcgcagtgat agtgccgtca ggcgagcggg
     3301 ctcggcgaac gtgagcacga tgttctcggg cgcggcattc agtacggcgc cattcgctgg
     3361 cacggacttc tcgagatgtg tatgagcacg ggcgccactg ctccaggcaa ggagcaccag
     3421 ggcagcgagg agtgcagaaa gtgcaacacc agcttggatt ttcatctcgg gcgatctcct
     3481 gttcagcgag tcagaatgcc gcgtgcaggc ccagggtcca ctctacagca ccggcgctcg
     3541 cgccggcggc ccgcagccgc tccgccgtgc cgccgaagtt acgcagccag acgagtccta
     3601 cgtagggacc gatatcgcgt cgcacttcga atcgtaagcg ggcgctgagc tccgcctcgg
     3661 caaggcccga gcccatttgt cgctggggat cgggccgacc gtacagattg gcttcggcga
     3721 acggctccag aatcaaccgc tgggtcagga gcagctcgta ttgcactttc agccgtgcgg
     3781 cggtccgccc ctcatcggag gcgtacagcg tcacttcggt ctcgacccat tgcggagcca
     3841 gtccctgcag ccccagggcc acccagctgc gcgactgccc ttgcgccagg tcctgcctga
     3901 gtccggcttg cacgttccac cagggcgaga cgacccggtt ccagagcaag tccacgctgg
     3961 tgttacgcag gccacccgat ccggccgcat agccgccctc tgttttcacc cagagcttgt
     4021 cgtagtcgcc gccataccag gcctcggcgt cccaggcggc atccggctcg ccggtcggtg
     4081 cacttcgcca ctccagctgg tccacgcgca cgccaccaaa gcgtgcggtg tcatccatct
     4141 gcatggtcgc tgccatgtcc tgaaaggaca tctgctgcgg cagaggcgat gcggtgtgca
     4201 tcgccagcat cgcggtcagt gccgccggag ccgcgcgcac gccacgcggg ctcacgcgac
     4261 gatcacctct cggaacatgc cggcttccat gtggtacagc agatgacagt ggaatgccca
     4321 gtgtccgagc gcacccgcgg tcacctcata ctgcagctcc tgaccgggct gtacggcggt
     4381 cgtatgcttg cgcaccagga aagcgccatc ggccgactgc acttcactcc acatcccgtg
     4441 caggtggatc ggatgcgcca tcatgctgtc attcaccaga acgattcgca gccgctctgc
     4501 gttcctgaac agcaacggca cactctcgga gaacttacgg ccgtcgaacg accagacgta
     4561 gcgttgcatg tgccctgtca gatgcaagcg gatttcgcgc cccgcctgcc ggccatcgac
     4621 cggtccatcc agggtgtgca agtccgcata gctcaatact cgccggccgt tgttccggag
     4681 cccgacgccc ggatcatcca gacggctcgc cggttggatt gcgacggcgt caacattggg
     4741 gccaaagtgc gcgggagcct gatccgcatt gcccatggcc atgtccgcca tgctgagcaa
     4801 gggacgcgga tccagaggcg gaatctgcgc ctgcatgccc gccaccggag cgagcgtgcc
     4861 gcgc
//
LOCUS       DASRHN010000218        12030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2378192, whole genome shotgun sequence.
ACCESSION   DASRHN010000218 DASRHN010000000
VERSION     DASRHN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378192"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..984)
                     /locus_tag="VFB37_08415"
     CDS             complement(1..984)
                     /locus_tag="VFB37_08415"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HZO22518.1"
                     /translation="MADSRPYPNFRYSRSCAASMLVAWLACCALPLCAWAGRAVRVYE
                     VDIRGGQSPAALQQAMREALVRATGRRESAADPAFASLIAEAPNYVKAYTQGPRGESQ
                     VVFDGAAVERAIAAAGRGVWDKDRPFTLVILYPPPPRTAEDVTRNELEQTAMARGLPV
                     SLVPLSPLDSAGAELGSDALMQTAQKYGGDAVLVGRSDTGAPPGQWQWTLHTNFTSQS
                     WSGPLSAGIDGAVDTLAPAQGSSLAETEIRAQIEIQGVSTLSDYATVQRMLESVPGVR
                     RASVAEANGASVTFDVLVRGGADAINRVLGSSAHFARADSSNARLVLQYRP"
     gene            1106..2140
                     /gene="purM"
                     /locus_tag="VFB37_08420"
     CDS             1106..2140
                     /gene="purM"
                     /locus_tag="VFB37_08420"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZO22519.1"
                     /translation="MKEAPGITYRDSGVDIDAGDELVERIKPIVRRTQRPEVLAGVGG
                     FGALVELPQGRYRHPVLVSGTDGVGTKLRLAIETGRHDSIGIDLVAMCANDVVVQGAE
                     PLFFLDYYATGKLRVDVAEIVIRGIAEGCAQAGAALVGGETAEMPGMYAGDDYDLAGF
                     CVGVVEKAAIIDGSQIRAGDAVIGLASSGPHSNGFALIRKLIGIAGANAATTLESGLL
                     FDRLLAPTRIYVKSLLELIRAVPVHGLAHITGGGLTDNIPRVLAAGLEVVLERQRWHR
                     DPVFEWLQRTGRIDAREMYRTFNCGIGMVAIVAPGEAQAAIDLLSARGERATLIGEVR
                     RGERGAVILE"
     gene            2137..2814
                     /gene="purN"
                     /locus_tag="VFB37_08425"
     CDS             2137..2814
                     /gene="purN"
                     /locus_tag="VFB37_08425"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZO22520.1"
                     /translation="MSNTTRTALRLAVPISGRGSNMVAIARACRDKRIAAQVSIVIAD
                     RPSAGGVALAREMGIDAQVVPWSSATGGPAFERALIECIDAHHPDLIILAGFMRILSR
                     ELVEHYAGRMLNIHPSLLPAYRGLQTHVRVLQGGDAWHGASVHFVTAELDGGPVILQS
                     RVPVKPDDTEASLAARVLSTEHEIYPRVIGWIADGRLAWCGGRPSLDGEPLQQPVVEN
                     FGALQDD"
     gene            2821..3546
                     /locus_tag="VFB37_08430"
     CDS             2821..3546
                     /locus_tag="VFB37_08430"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZO22521.1"
                     /translation="MITRNGKHILPAGLLSLTALACQGFALADELQPFEASYSWQWHG
                     MTVAVSTLKLEKREDTWVYRSRSEPRGIGRMFSERPTQESILRVTASGTEPLSYKADD
                     GTSSTKRDVDVRFDWLHGHVTGVYEDTPVDIPVRAGLQDDMSVQIALMVDLLRGHPPA
                     QVLLLDRDAVREYSYTREGEETLDTPLGRVSTVIYQSHKQGSPRVDLFWCAPAHGYIP
                     MRVEQRRLGEVQWTMQIQKLKRD"
     gene            complement(3550..3768)
                     /gene="dcd"
                     /locus_tag="VFB37_08435"
                     /pseudo
     CDS             complement(3550..3768)
                     /gene="dcd"
                     /locus_tag="VFB37_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(3781..4602)
                     /locus_tag="VFB37_08440"
                     /pseudo
     CDS             complement(3781..4602)
                     /locus_tag="VFB37_08440"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
     gene            complement(4576..4926)
                     /gene="dcd"
                     /locus_tag="VFB37_08445"
                     /pseudo
     CDS             complement(4576..4926)
                     /gene="dcd"
                     /locus_tag="VFB37_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(4952..6049)
                     /gene="apbC"
                     /locus_tag="VFB37_08450"
     CDS             complement(4952..6049)
                     /gene="apbC"
                     /locus_tag="VFB37_08450"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HZO22522.1"
                     /translation="MMDDASLNALRAALDSYADPYLGESLGAAQAVQDLRPAGEGARA
                     RIRLGFPVGGYHDELAEGLKRHLAGAGITIPLALELVAEIRAHAVQRNLKPLERIKNI
                     VAVASGKGGVGKSTVAVNLALAWAAQGARVGMLDADIYGPSQPLMLGLAGEHPGSPDG
                     KHLKPVAAHGIAAMSIGFLVDAEQPMVWRGPMVTQALSQLLSETDWGELDYLVVDMPP
                     GTGDIQLTLSQRVPVAGAVIVTTPQDIALADARKGLRMFEKVSVPVLGIVENMSVHVC
                     SNCGHTEHIFGAGGGARMAAQYGVALLGELPLDAHIREEADSGRPSVIAAPASARAQA
                     YFAMARRTAAALSLRARDRSSVFPKIVVEES"
     gene            6290..8731
                     /locus_tag="VFB37_08455"
     CDS             6290..8731
                     /locus_tag="VFB37_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZO22523.1"
                     /translation="MSFFTNLRADRLITEIRSTNDPSSPATQKAVARLRDLGPGAIEP
                     VFAALPDASKSATVALVEVLAGLVSQKTFPQFVRGLVEGSARVIAGISWALTSNRSYP
                     PHLLIEALATPGISKPAILEVIAAQKTRFGVRELLSAAYAQEPNEKAALFRIIGEIAD
                     ASAVPELVGRVQGKDPIARVHIINLLARFNTPEVQTSLQAQLRDPNKLIRGATLSALQ
                     RMDGPIDIERICALLKDPEIDVQNKAIDVVIKANHPETIRYLIEVLKDENENARRAAV
                     EVLNEVGNAKSVKYLLEALKDSDWWVRSRAADALGKIGGPRVIEAVLQLVRDKDDDIR
                     RAAIEILNQTKDERAVESLIQATHDADWWVSERAVDALAEIGSKRALPRLTEMLQAGN
                     TKGLPIVVRAIGRLGDSKFVDMLMPLVSRSEREVRLEAIQALAKLVDERRVDQLRLEL
                     QTQASSNDQTIARMAASAMAELDNRLLGGLTPTGTGPTGGTVGHPSGIPPSAVRPPLP
                     QPPEGKTLLMSETAVAHATKQGEAAARLDIGTLKPGDIIEGRYKYIDRIGRGAFGTVL
                     LMEDTVVDERLILKFLNPNVSNDEEVMKRFVHELRYSRKITHKNVIRIYDFLYMQGNY
                     AISMEYFPSHTLGSEVVNEKPLELKRAIRFGIDMCTGMTVAHQVGIVHRDLKPANVLI
                     NQEGLLKIVDFGVAAAHREGDTQLTKTGYVIGSPKYMAPEQILGKKVDERADIYALGV
                     IMYEMLSGVPPYSRGDHMSVMYQHVQGKARAAREVNPNLPQGLSELIAKAMSVDKSKR
                     HQSMEELRADLERYA"
     gene            8877..9992
                     /locus_tag="VFB37_08460"
     CDS             8877..9992
                     /locus_tag="VFB37_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZO22524.1"
                     /translation="MSRIDAFLKLGSQQGCSDVHLAVGVPPMLRMHGDLLPIKFRDLR
                     PGELEGYISEILTCAQSEIFNKGNDLDFSYVSSEGGRFRVNVYRKDTGVGATFRAIPS
                     EVPTLEKLALPPIVTKLCDFHQGMILVTGSTGTGKSTTLAAMIDYLNQTRRLNIISLE
                     DPIEFVHRSKNSQVIQRELGTHIPSFAEGVRAAMREDPDVILVGELRDAETISMAMTA
                     AETGHLVLGTLHTTSAVKTIDRIIDALPVEEREQTKSFLAQSLLAVVTQILVKTADGR
                     GRRAMCEVLMMTKAIAKLIQTEQTHQIPTQMQTGRDLGMSLLDQALLAAINAREIDPD
                     DAYTYAMDKRAFQKFVTDTTMLPKIEVAAANATAASG"
     gene            10002..11063
                     /locus_tag="VFB37_08465"
     CDS             10002..11063
                     /locus_tag="VFB37_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZO22525.1"
                     /translation="MAEIDAFLSEILVKRGSDLHFIAGDQPRIRLFGELTPLRTERLA
                     AEYVKAALYEIMPKPAVDRFEARDGADFAYTLADRGRFRVNVLRQLSGMGAVFRAIPS
                     KALSLDELAMPEAVRQLCRANNGLILVTGKTGSGKSTTLAAMIDDINSRVKGHILTIE
                     DPIEFVHQRKSCLISQREIGVHSPSFAAALHSALREDPDVILVGELRDLETMGIAVTA
                     AEMGILVMGTLHTNGAAPTVDRMINVFPSDKQSHVRTMLSTSLRGVVSQQLLQKADKQ
                     GRVAALEILVNTPASQNLIRQGKLDQLENTMQSGGQFGMRTMDTAIQQLLEQRQITGK
                     EAYKKAIHKAKFEPVKDAG"
     gene            11105..>12030
                     /locus_tag="VFB37_08470"
     CDS             11105..>12030
                     /locus_tag="VFB37_08470"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZO22526.1"
                     /translation="MPRKLFVTTALPYANGPFHIGHIMEYIQADIWVRYQRMLGNEVH
                     FVCADDAHGAPIMLKAEAEGIPPQELVRRMAATRPRYLEGFDIGFDHWYSTDSPENVA
                     LSQDIYRRLKAAGFVYLKAIEQFYDPVKGMFLADRYIKGECPNCHSKDQYADACEVCS
                     IVNSPTDLIEPYSTLSGARPVMKSSEHYFFRLSDPRCVQFLTAWLDTPERLQPQVINK
                     AREWLSGKGEDALGDWDISRDAPYFGIPIPDAPGKFFYVWLDAPIGYLAALKSYFDSG
                     KAARAGERRTFEQFLQAPDTEQVHFIGKDIIY"
BASE COUNT         2241 a         3936 c         3798 g         2055 t
ORIGIN      
        1 tcagggccga tactgaagca cgagccgcgc attcgatgag tccgcccggg cgaaatgagc
       61 cgagctgccc agcacacggt tgatcgcatc cgctccaccg cgcaccaaca catcgaacgt
      121 gacggaagca ccgttggcct ctgcgacgct ggcgcggcgc acgcccggca cactctcgag
      181 cattcgctgc accgtagcgt agtcgctcag agtggacact ccctggatct cgatctgtgc
      241 gcgaatctcg gtttcagcga gagaactgcc ctgggctgga gccagtgtgt caactgcgcc
      301 gtcgatgcct gcgctcagtg ggccactcca gctctggctc gtgaaattcg tgtgcagcgt
      361 ccattgccat tgtccggggg gcgcgccggt gtcgctccga cccaccagca ccgcatcgcc
      421 tccgtacttc tgagcggtct gcatcaatgc atcactgccg agctcagcgc ccgccgagtc
      481 caaaggcgac agcggcacga ggctcacggg caagccgcgc gccatggcgg tctgctcgag
      541 ctcattgcgc gtcacatctt cggctgtgcg cggcggcggc ggatagagga tcaccagtgt
      601 gaaaggacgg tccttgtccc acacgccgcg ccccgcagca gcaattgccc gctcgactgc
      661 ggccccgtcg aaaacgacct gactctctcc gcgcggtccc tgtgtatacg ccttcacgta
      721 attcggggct tccgcaatca ggctggcaaa ggccggatcc gcggcggact cgcgccgtcc
      781 ggtcgcccgc acgagagcct cgcgcatcgc ctgctggaga gcggccgggg actgcccgcc
      841 ccggatatct acttcataga cgcggaccgc ccggcctgcc caggcgcaca gcggcagcgc
      901 gcaacaggca agccacgcca cgagcatcga agctgcgcaa gagcgcgaat atcggaagtt
      961 cggatagggc ctgctgtcag ccatgcgcaa agggtaagcg tgcatcggcg cataggtaaa
     1021 ttggccgcgc gcccggggac cggcggaatg tagaatatca cagggtcgga ggctccccgc
     1081 ccgcgcagca ttccctgcac agatcatgaa agaagcaccg ggcatcacct atcgcgattc
     1141 gggtgtcgac atcgacgccg gcgatgagct tgtcgagcgt atcaagccga tcgtgcgccg
     1201 cacgcaacgc ccggaggtgc tggccggtgt cggtggattc ggcgctctgg tcgagctgcc
     1261 tcagggccgc tatcgtcatc cggtactcgt ctccgggacc gacggcgtcg gcacgaaact
     1321 gcgcctcgcc atcgagaccg gccgccacga cagtatcggc atcgatctgg tcgcgatgtg
     1381 cgccaatgac gtggtcgtgc agggagcgga gccgctcttc tttctggact actacgccac
     1441 aggcaagctg cgtgtggatg tggccgagat cgtcatccgg ggtatcgctg aaggctgtgc
     1501 gcaagccggc gcggccctgg tgggcggcga aacggccgag atgccgggca tgtatgccgg
     1561 tgatgactac gatctcgccg gcttctgcgt cggcgtggtg gagaaagcag cgatcatcga
     1621 tggcagccag atccgtgccg gcgatgccgt gatcgggctt gcctcctccg gaccgcactc
     1681 caacggcttt gcgctcattc gcaagctcat cggcatcgcc ggagcgaatg ccgcgacgac
     1741 gctcgagtcc ggactgctgt tcgacagact cctcgctccc acccggatct acgtgaagtc
     1801 actcctggag ctcatccgcg ccgtccctgt acacggcctt gcgcacatta ccggcggcgg
     1861 cctcacggac aacattcccc gtgtgctcgc tgcgggactc gaagtcgtgc tcgagcgcca
     1921 acgctggcat cgcgacccgg tgttcgaatg gctccagcgc accggccgca tcgacgcgcg
     1981 cgagatgtat cgtactttca actgcggtat cggcatggtg gccatcgttg cacccggcga
     2041 ggcccaggct gcgatcgacc tgctgagcgc ccgcggcgag cgcgcgacgc tgattggaga
     2101 agtccgccgc ggcgagcgcg gcgctgtcat tctcgagtga gcaacactac gcggactgct
     2161 ctgcgcctcg cggttccgat ctccggtcgt ggctccaaca tggtggcgat cgcgcgcgcc
     2221 tgccgcgaca agcggatcgc cgcgcaggtg agcatcgtga tcgccgatcg cccgagcgcg
     2281 ggcggcgtcg ctctcgctcg cgagatggga atcgacgctc aggtggtgcc gtggagctcc
     2341 gccacggggg gcccggcttt cgagcgcgcc ctcatcgagt gcatcgacgc tcaccacccg
     2401 gatctgatca ttctggccgg gttcatgcgc atcctctctc gcgagctcgt cgagcactac
     2461 gccggccgta tgctcaacat ccatccctcc ctcctgcccg cctatcgagg gttgcagacc
     2521 catgtccgcg tgctgcaggg cggagatgcc tggcacggag ccagcgttca cttcgtcaca
     2581 gccgagctcg atggtggccc cgtcatcctc caatccagag tgcccgtgaa gccggacgac
     2641 accgaagcca gccttgctgc gcgggtgctg tcgaccgagc atgagatcta cccccgggtg
     2701 atcggctgga ttgctgacgg tcggctcgcc tggtgcggcg gccggccatc attggatggc
     2761 gagcctctgc agcaacccgt cgtggagaat ttcggcgcgt tgcaggacga ttgagcgatc
     2821 gtgatcacgc gcaacggcaa gcacattctc cccgcagggc tgctgagcct gaccgcgctc
     2881 gcctgccagg ggtttgcgct ggccgatgag ctgcagccct tcgaggcctc gtactcgtgg
     2941 cagtggcacg gaatgacggt cgccgtttcg accctcaagc tcgaaaagcg cgaagacacc
     3001 tgggtctatc gctcgcgcag cgagccgcgc gggattgggc gcatgttttc cgagcgcccc
     3061 acccaggaaa gcattctgcg tgtgaccgcc tccggcaccg agccgctcag ctacaaagcg
     3121 gatgacggca cctcctcgac aaaacgcgac gtggacgtcc ggttcgactg gctgcacggc
     3181 catgtcacag gagtctacga agacacgccg gttgacatcc ccgtgcgggc cggtctccag
     3241 gatgacatgt cggtgcagat tgcgctgatg gtcgacctgc tgcggggcca tccgcccgca
     3301 caggtactgt tgctcgaccg cgatgcggtc cgggagtaca gctacacgcg cgaaggcgag
     3361 gaaacacttg atacgcctct agggcgcgtg tccaccgtca tctaccagag tcacaagcag
     3421 ggctcgccgc gggtggacct gttctggtgc gcacccgctc acggctacat tcccatgcgc
     3481 gtcgaacagc gccgcctggg cgaggtgcag tggaccatgc agatccagaa gctcaagcgc
     3541 gactgaagat caggtcttgg gcagcgtcac cccgcgctgg cccatgtact tcccgccgcg
     3601 atcgcgatac gaggtggaac agacctcgtc cgactcgaag aacagcatct gggctacccc
     3661 ttcgttcgcg tagatttttg ccggcagcgg cgtcgtgttg gagaattcca gggtcacatg
     3721 gccttcccat tcgggttcca aaggcgttac gttcactatg atgccgcagt tgcggacaaa
     3781 gacacccgct tcgagggcga aatttcccgt ctctggcaca gtgaggcagt agacatcatg
     3841 atcgcccgga aggtggcgca cagaggcgac cttgtgattg cggtagcggg cgagcccgcg
     3901 aaattcggcc actgcttccg gaaaacgcct gaagactgag cggtcgcatt ccaggagctg
     3961 cgccgcgcct cggatcgacc cggtgacgtg ccccaggcgg ctcaaggcag gacggatggc
     4021 gaggccgtgc tcggttggac cgaaatcctc accgtaactc tcggcattgt gcagccggat
     4081 atgctccgct gcacccatcg gtatgatgtt agtgggtcga ttattgcgtc gatcaaaatc
     4141 gccatgatgg cggtgcgtac cggcgctctc cgcatacacc tcgtggcgta gattccactc
     4201 atcagccagc cggtgagtgg ggcacatgac attctctatt ggttgataga cagcctcata
     4261 gccacggaac agatcgcgat aaaggggcat tagcgcatcg ccaggccgga gcctatgcgc
     4321 ctcgatcatc cgtccatcgc gtcgaatgaa gtggtgatca ggcgtggtgt ggatcttctc
     4381 gccgctgtct agcctgatct ccagcagggc gtcgcgtccg atgaaacgcg gaacgtcgag
     4441 aagcgtgacc agcacacgac ctgccgggcc tattccgtag ccccagtaca gctgtccgac
     4501 ctcgtgaccg cgggccatct gctcgagggt agccgaggtc ccatcgacga gcgccacacg
     4561 tgtatcgccc cggaaacagc gcgcgaacgt cgatttcccc agggtcacga ccagcacgtt
     4621 gcgcggcaca cggaagtact cgacggtgcg cgccagcgcg aaggagttgg gcggaatgat
     4681 gcagacatcc gcgcgcaggt ccacgaagct tttttcgtcg aaattcttcg gatcgacgat
     4741 ggtggagttg atgttggtga agatcttgaa ctcgctggcg cagcgcacgt cgtagccata
     4801 gctcgaggtg ccgtaggaca cgattttcct gccctccacg tagcggacct ggtccggctc
     4861 gaacggctcg atcatgcggt gctcctgtgc catccgccgg atccagttat cggacttgat
     4921 gctcatcgcc tgctctcccc gcggaaaatc ttcagctctc ttcgaccacg atcttcggaa
     4981 atacgctgct gcggtcgcgc gcgcgcaacg acaacgcggc cgccgtgcgg cgcgccatgg
     5041 cgaaatacgc ctgcgctcgc gcggaggccg gcgctgcgat cacactcggg cggccactat
     5101 cggcttcctc gcgaatgtgc gcatcgagtg gtaactctcc gagcaacgcc acaccgtact
     5161 gcgccgccat gcgagcgccg ccgccagctc cgaagatgtg ctccgtgtgg ccgcagttgg
     5221 agcagacgtg cacactcatg ttctcgacga ttcccagcac gggcaccgaa actttctcga
     5281 acatcctgag gcccttgcgg gcatcggcga gtgcgatgtc ctgcggtgtc gtgacgatca
     5341 cggcaccggc caccggaacc cgctgcgaca aagtgagctg gatatcaccc gtgccgggcg
     5401 gcatatccac caccagatag tcgagctcgc cccaatcggt ttcggacagc agctgtgaca
     5461 gagcctgcgt gaccatcgga ccgcgccaga ccatcggctg ctcggcatcg accaggaatc
     5521 cgatcgacat ggcggcgatg ccgtgagcgg ccacgggctt cagatgcttg ccatccgggg
     5581 agccgggatg ctcgcccgcc agccccagca tcagcggctg gctcgggccg tagatgtcgg
     5641 cgtccagcat gccgacgcgc gccccctggg cggcccaggc aagcgcgagg ttgacggcga
     5701 ccgtggactt cccgacgccg cccttgcccg aggcgacggc gacgatgttc ttgatgcgct
     5761 cgaggggctt gaggttgcgc tggacggcgt gagcccggat ctcggccacg agctcgaggg
     5821 ccagggggat cgtgatccct gccccggcca gatggcgctt caggccctcc gccagctcat
     5881 cgtggtagcc gcccacagga aaacccagcc ggatgcgggc tctggcgccc tccccggcgg
     5941 gacgcaggtc ctgcaccgct tgagcggcgc ccaaagactc cccaaggtag ggatccgcgt
     6001 atgaatccaa agccgcgcgc agtgcgttta ggctggcatc atccatcatg ttgagaggcc
     6061 ggggaggaca aagggttcgg attgtagagg ctgcggcgag cgcgcgctcg gcggccgcgt
     6121 cctacagccg gcctcgtcca aggctgtggc ataatcctgc tgcccgcagg tgcgagctcg
     6181 ccagccgccg cgccggcagc ggacaacccg agcaaaactg gcggaggcac caactaccgc
     6241 gggcgtcgga atccacagcg catgcccacg gggaccgacc cggagctgaa tgagcttttt
     6301 cacgaatttg cgtgcggacc ggctgatcac ggaaatccgc tcgacgaacg acccgtccag
     6361 ccccgctacg cagaaggccg tcgcgcggct gagggacctg ggaccggggg cgatcgagcc
     6421 cgtgtttgcc gcgctgccgg acgcgagcaa gagcgccacc gtcgcactcg tcgaggtgct
     6481 ggcggggctc gtcagtcaga agacgtttcc gcagttcgtg cggggcctcg tggaaggcag
     6541 cgcccgggtc atcgccggta tctcctgggc cttgaccagc aaccgcagct atccccccca
     6601 cctgctgatc gaggcactgg cgacgcccgg gatctccaag cccgccatcc tggaagtcat
     6661 cgcggcacag aagactcgct tcggagtgcg tgagctgctc agcgccgcct acgcgcagga
     6721 gcccaacgag aaggcggcac tgtttcgcat catcggcgag atcgccgatg ccagcgccgt
     6781 acccgagctc gtcgggcggg tgcagggcaa ggatcccatt gcccgcgtgc acatcatcaa
     6841 cctgctcgcg cgcttcaaca ctcccgaggt acagacttcg ctgcaggcgc agcttcggga
     6901 ccccaacaag ctgatccgcg gagccactct ctccgcgctg cagcgcatgg acgggccgat
     6961 cgatatcgag cgcatctgcg cgctgctgaa ggatccggag atcgatgtcc agaacaaggc
     7021 gatcgatgtc gtcatcaagg cgaatcatcc cgagacgatc cgctatctca tcgaggtgct
     7081 gaaggacgag aacgagaatg cgcgccgcgc agcggtggag gtgctcaacg aagttggcaa
     7141 cgcaaagtcc gtgaaatacc tcctcgaggc gctcaaggac agcgactggt gggtacgcag
     7201 ccgcgccgcg gatgccctcg gaaagattgg cggcccgcgc gtgatcgaag cggtgctgca
     7261 gctggtgcgc gacaaggacg atgacatccg ccgagcagcg atcgagatcc tcaaccagac
     7321 caaagacgag cgggcggtcg agtccttgat ccaggcgact catgatgcgg actggtgggt
     7381 gagcgagcgc gccgtggacg ccctcgccga gatcggcagc aagcgcgcgc tgccgcggct
     7441 gaccgagatg ctgcaggcgg gcaataccaa ggggctgccc attgtggtcc gggccatcgg
     7501 caggctcggg gattcgaagt tcgtggacat gctgatgccg ctcgtgtccc gctcggagcg
     7561 cgaggtgcgg ctcgaagcca ttcaggccct ggcgaaactg gtggacgagc gccgcgtcga
     7621 tcaattgcgg ctcgagctgc agacccaggc gtcctccaac gaccagacca tcgcgcgtat
     7681 ggccgcttcg gccatggcag agctggacaa tcgcctgctg ggaggcctca caccgaccgg
     7741 tacgggcccc acggggggca ccgtcggcca tccctcgggc atacctccat cggctgtgcg
     7801 accacccctg ccgcaaccgc ccgagggcaa gacgctgctc atgtcggaga cggcggtcgc
     7861 gcatgccacc aagcagggcg aagccgccgc gcgactcgac atcggcaccc tcaagccggg
     7921 cgacatcatc gaaggacgct acaagtacat cgaccgcatc ggccggggcg ccttcggcac
     7981 cgtgctgctg atggaggata cggtggtcga tgagcggctg atcctgaagt tcctcaatcc
     8041 aaacgtctcg aacgacgaag aggtgatgaa gcgcttcgtc catgagctgc gctactcgcg
     8101 caagatcacg cacaagaacg tgatccgcat ctatgacttc ctctacatgc agggcaacta
     8161 cgccatctcg atggagtatt ttccctcgca tacgctcggc agcgaggtcg tcaacgaaaa
     8221 gccgctcgag ctcaagcgcg cgatccgctt cggcatcgac atgtgcaccg gcatgaccgt
     8281 ggcacaccag gtcggaatcg tgcatcgcga tctgaagccg gccaatgtcc tcatcaacca
     8341 ggaggggctg ctcaagatcg tggacttcgg tgtggccgcc gcacaccgcg aaggcgatac
     8401 gcagctcacg aagaccggtt acgtcatcgg ctcgcccaag tacatggcgc ccgagcagat
     8461 cctcggcaag aaggtcgatg agcgcgccga tatctatgca ctgggtgtca tcatgtacga
     8521 gatgctctcg ggagtgcccc cttattcacg cggcgatcac atgtcggtga tgtatcagca
     8581 cgtgcagggc aaggcgcgcg cagcccgcga ggtcaatccg aatctgccgc agggcctctc
     8641 ggagctgatc gccaaggcga tgtccgtgga caaatcgaag cggcaccagt ccatggaaga
     8701 gttgcgcgcg gacctcgagc gatacgcctg aggggtcgcg gcggcagcgg ggacggccgc
     8761 tcttgccaaa cttcgtgcgt tacgtcaaat ttccggccac aacttattga ttccgctgcc
     8821 tgccccgggc taaacaacct gcagtatccc gaggcttctc gagaggggtg gtcgtcttgt
     8881 cgcgtatcga cgcgtttctc aagcttggct cgcagcaggg ctgctccgac gtacatctgg
     8941 cggtcggcgt gccgcccatg ctgcgcatgc acggcgatct gcttcccatt aaattccgcg
     9001 atctgcgtcc cggggagctc gaaggctata tcagcgagat cctcacttgc gcgcagtccg
     9061 agatcttcaa caagggcaac gacctcgact tctcatacgt ctcttccgag ggaggccgct
     9121 ttcgcgtcaa tgtctaccgc aaggacacgg gcgttggggc caccttccgc gccatcccct
     9181 ccgaggtgcc gactcttgag aagctggcgc tgccgccgat cgtcacgaag ctctgcgatt
     9241 tccatcaggg catgatcctg gtcaccggct ccaccggaac cggcaagtcc accacgcttg
     9301 ccgcgatgat cgattacctc aaccagaccc gccggctgaa catcatcagc ctcgaagatc
     9361 ccatcgagtt cgtgcatcgg agcaagaaca gtcaggtcat ccagcgcgag ctcgggacgc
     9421 acattcccag ctttgcggaa ggtgtgcgcg ccgcaatgcg tgaggatccg gatgtcatcc
     9481 tggtcggtga gctgcgtgac gccgagacca tctccatggc catgaccgcc gcggagaccg
     9541 ggcacctcgt gctcggcacg ctgcacacga cgagtgccgt gaagaccatc gatcgcatca
     9601 tcgatgcact accggtcgag gagcgggagc agaccaagag cttcctcgcg cagagcttgc
     9661 tggccgtggt gacccagatt ctggtcaaga cagccgacgg acgcggccgc agggccatgt
     9721 gcgaagtgct gatgatgacc aaggctatcg ccaagctcat ccagacggag cagacgcacc
     9781 agattccgac tcagatgcag acgggccgcg atctgggcat gtcgctgctg gaccaggcgc
     9841 tgcttgcggc catcaacgca cgtgagatcg atccggacga cgcctacacg tatgcgatgg
     9901 acaagcgcgc cttccagaaa ttcgtgaccg acacgaccat gctgcccaag atcgaggtag
     9961 ccgccgccaa tgcgacggcg gcctccgggt gagggagcaa gatggccgag attgacgctt
    10021 tcctcagcga gatcctcgtg aagcgcggct cggacctgca cttcatcgcc ggcgatcagc
    10081 cgcgcatccg cctgtttggc gagctgacgc ccttgcggac ggagcgcctc gcggccgagt
    10141 acgtcaaggc ggctctgtac gagatcatgc cgaagccggc cgtcgatcgc ttcgaggcca
    10201 gggatggtgc ggacttcgcc tacacgctgg ccgatcgcgg ccgctttcgc gtcaacgtac
    10261 tcaggcagct gagcggcatg ggcgccgtgt tccgcgcaat cccatccaag gcgctgtcgc
    10321 tggatgagct cgcgatgccc gaggcggtgc ggcagctgtg ccgggccaac aacggcctga
    10381 tcctcgtgac gggcaagacc ggctccggaa aatcgacgac gctcgcggcg atgatcgacg
    10441 atatcaactc gcgggtgaaa ggccacatcc tcaccatcga ggatccgatc gagttcgtac
    10501 accagcgcaa gagctgcctg atcagccagc gcgagatcgg cgtgcacagc ccgagcttcg
    10561 cggcggccct gcattcggcc ttgcgggagg accccgacgt gatactggtt ggcgagcttc
    10621 gtgacctgga gacgatgggc atcgcagtca cagccgcgga gatgggcatt ctggtgatgg
    10681 ggacgctgca taccaacggc gccgccccga ccgtggaccg catgatcaac gtattcccgt
    10741 ccgacaagca gtcccacgtg cgcacgatgc tgtccacttc gctgcgcggc gtcgtctcgc
    10801 aacagctgct gcagaaagcg gacaagcagg ggcgcgtagc cgctctcgag atcctggtga
    10861 acacgccggc ctcccagaac ctcatccgcc agggcaagct ggatcagctc gagaacacca
    10921 tgcagtccgg cggtcagttc ggcatgcgca ccatggatac ggcgatccaa cagctgctcg
    10981 agcagcggca gatcacgggc aaggaggcct acaagaaagc gatccacaag gcgaaattcg
    11041 agcccgtcaa ggacgccggc tagacccggt cacatcgccg gcgcgcggca ggtacactag
    11101 gcggatgccg cgcaagctgt ttgtcacgac cgccctcccc tatgccaacg gcccgtttca
    11161 catcgggcac atcatggagt acatccaggc cgacatctgg gtgcgttacc agcggatgct
    11221 cggcaacgag gtgcatttcg tctgcgccga tgacgctcac ggcgcgccga tcatgctcaa
    11281 ggcagaggcc gaaggtattc cgccgcagga gctcgtgcgg cgcatggccg cgacccggcc
    11341 ccgatatctc gaaggcttcg acatcggctt cgatcactgg tactcgaccg attcgccaga
    11401 gaacgtcgcg ctctcgcagg acatttaccg gcggctgaaa gctgcgggct tcgtctacct
    11461 caaggccatc gagcagttct acgatccggt caagggcatg ttcctggcgg accgctacat
    11521 caagggtgag tgtcccaact gtcactcgaa ggatcagtac gcagacgcct gcgaggtctg
    11581 cagcatcgtc aactcaccca ccgatctcat cgagccctat tccacactgt cgggggcccg
    11641 gccggtgatg aagagctccg agcattactt cttccggctg tccgatccgc gctgcgtgca
    11701 gtttctcacc gcctggctcg acacccccga gcgcctgcag ccccaggtca tcaacaaggc
    11761 gcgcgaatgg ctgagcggca agggggagga tgcgctcggg gactgggaca tctcgcgtga
    11821 tgcaccgtat ttcggcatcc cgatacccga tgcgcccggt aagttctttt atgtgtggct
    11881 ggatgctccc atcgggtatc tggcggccct gaagagctac ttcgacagcg gcaaggcggc
    11941 ccgcgccggc gagcgacgca ccttcgagca gttcctgcag gcgcccgata ccgagcaggt
    12001 gcatttcatc ggcaaggaca tcatctactt
//
LOCUS       DASRHN010000219         7361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_565778, whole genome shotgun sequence.
ACCESSION   DASRHN010000219 DASRHN010000000
VERSION     DASRHN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565778"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFB37_08475"
     CDS             <1..262
                     /locus_tag="VFB37_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZO22527.1"
                     /translation="GYGYGYGWGWGWGWGPGWGPGFAGGWGWDYPYVYHEGIISVDLY
                     DARTSQPLWHASVNQSLAGATGDKADQKIRAAVAALFTKYPR"
     gene            complement(373..711)
                     /locus_tag="VFB37_08480"
     CDS             complement(373..711)
                     /locus_tag="VFB37_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZO22528.1"
                     /translation="MNPHRSGSLSALLGMTLVSLMAPAAQAAAPESTGVVMKNFMFVP
                     TSLTVDVGSTVTWTNQDGEPHTVISDSGLFRSAALDTNDKFTFRFEQPGTYHFVCSIH
                     PQMVGTIIVK"
     gene            complement(757..1698)
                     /locus_tag="VFB37_08485"
     CDS             complement(757..1698)
                     /locus_tag="VFB37_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZO22529.1"
                     /translation="MTDEISLPGRRKALRCMALGGAGTLFALAGGVFTPIDLAVAATD
                     PSRAIPAGKPLFVQISDTHIGFNKEANPDVDATLNRAVDLVNGLPDQPALVIHTGDIT
                     HLSRPTEFDLAQQMLSRLRASELHTVPGEHDVTDPTVSEYFNRFRKASERRGYYSFDH
                     AGVHFIALINVLQFKPGGLGTLGDEQLAWLAEDLRGRSASTPIVVFAHMPLWTIYQPW
                     GWGTADAEQLLSQLRRFGSVTVLNGHIHQIVQKVEGNVTFHTARSTAYPQPTAGDGPG
                     PGPLVVPAAQLPAMLGVTSVSVVAHPHTLRLTDRTLA"
     gene            1811..2467
                     /locus_tag="VFB37_08490"
     CDS             1811..2467
                     /locus_tag="VFB37_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HZO22530.1"
                     /translation="MSAIDPKPVGEPDGSGTRDKALDFWFEFGSNYSYLSVMRIEAAA
                     ARAAVRICWKPFLLSPIFRSLGWSSSPFVQQKEKGEYVWRDMARQCAKYGLPWKHPTS
                     FPRRALLPMRVALYGAREPWIGEFCRRIMCANFAEDQDIDSPRLVGPILEELGVAAGT
                     VLQGAQSETNKQLLREQTERARALGIFGAPTFFVGEEMFWGNDRLEDALAQCSAAPRG
                     "
     gene            complement(2488..3723)
                     /locus_tag="VFB37_08495"
     CDS             complement(2488..3723)
                     /locus_tag="VFB37_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HZO22531.1"
                     /translation="MKHDAAQIAALVIFALTYLAIAWGRFPGLRLDRTGAALAGATCM
                     IASGVLTLRQAYAAIDFDTVTLLLGVMIVVATLRLSGLFSMISTAVARHARRPLLLLV
                     WIVLVSGILSAFLVNDTICLALTPLVLEIVVALRRNPLPYLLAVAMASNAGSVATITG
                     NPQNMIVGSLAHIPYAVFAGTLAPVAAASLLLSIAVIAIAFPAEFRTAEVLQAAPPVG
                     APRASRALIVKAAVITAGMVGAFFWGFPPAAVAISAGVLMLLTRRLKPERIYAELDWP
                     LLLMFVGLFIVVAGLESVLTRQAHALAGALHLEHAPVLVGFTAVLSNIVSNVPAVLVL
                     RPFLGALPHPQHAWLLVAMSSTLAGNFTLVGSVANLIVARRAQALGTSIGFWAYFRVG
                     APLTILTLLIGTAWLAVID"
     gene            3884..5491
                     /locus_tag="VFB37_08500"
     CDS             3884..5491
                     /locus_tag="VFB37_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZO22532.1"
                     /translation="MILRAPVLKRSLLLAVAVSSAILAGNTYAQSEAHANVPHVKHVL
                     LLSVDGLHALDLSNYVASHKDSTLAALSRRGVTFTNNSTSTPSDSFPGLASLVTGGSP
                     ITSGLWYDDTYNRALSPPAQTDGLGNPGGNCPGKIGTNVAWDEAVDIDLTKLDGGGGL
                     NPNFLVRDPRNNCHTILPHQYLRVNTIFEVVRAHGGRTAWTDKHPSYEWTNGPSGKGV
                     EDFYGPEINSIPVPLPQISGCAVVPFQDPTPDDGWTNSFDDIKCYDSLHVQAVLNQID
                     GYTHDRTKKVGVPTLFGTNFQAVSVGEKLASDPVTGLKGGYTDVLGTPGEALAGELDF
                     VDQSLGRFVAEMRREGIFDSTLIVISAKHGQSPIDVTRRRGIGHGQPGATIGAPDAFD
                     ISDDGSLIWLTDQELTASVVANLSSSANQAALGIQEIFAGASLRNKFDDPGSDPRTPD
                     IILKVNTGVIFTGGTKLAEHGGFNEDDVHTALLVSFPDAEPASVSTATTNQQVAPTIL
                     QALGIDPDELQAVQREQIAPLPLRPQR"
     gene            complement(5529..6653)
                     /locus_tag="VFB37_08505"
     CDS             complement(5529..6653)
                     /locus_tag="VFB37_08505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO22533.1"
                     /translation="MEAIFDIKSVLAVLCLLTWGYLFLAHGRFWVSGPELPPANPAEP
                     PDVDIIVPARDEAQTIQPVMASLLAQSYAGRFRVILVDDSSSDATVALAGAAANLLIL
                     RAPAKPPGWSGKLWAVNQGVAASSAPVLLLTDADIVHDPRHLTSLVARLERPRAQMVS
                     EMVKLNCTSFAERMLVPAFVYFFQMLYPFTRVNEQRSRTAAAAGGTMLIRREALQAIG
                     GLAAIREALIDDVALAKALKRTGPIYLGHSALATSIRTYPGTADIWRMVSRTAFTQLR
                     HSGCLLALTLLGLAIVWWAPVAMAVLGRGWQRDCGVAACALAVISYMPTLVRYGRARL
                     WAITLPLIALFYMGATLDSAIQFWRGRGASWKSRAYGASH"
     gene            6969..>7361
                     /locus_tag="VFB37_08510"
     CDS             6969..>7361
                     /locus_tag="VFB37_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22534.1"
                     /translation="MLYFSRPIGGGVGPAMSHPTMGLKINQVRQMPNNGDPEATGEAM
                     QHRELINWQLGGRSAIRLSDMRLQLGHRVTWDLSRGSFGKTAPSITLEPGSLRGAAAT
                     APHTQLGGPRSAPSIERIGVTRTGERNMA"
BASE COUNT         1430 a         2292 c         2416 g         1223 t
ORIGIN      
        1 cggttatggc tatggctatg gctggggctg gggctggggc tggggcccag gctgggggcc
       61 ggggttcgcc gggggttggg gctgggatta cccctacgtc tatcacgaag gcatcatttc
      121 ggtggatctg tacgatgccc gcaccagcca gccgctgtgg catgcctcgg tgaaccagag
      181 tctggcgggc gccactggcg acaaagccga ccagaagatc cgggcggcgg tggcagccct
      241 cttcactaag tatcctcgat gaccctggta ccgggagacc ggtaccaggg cccgcctcaa
      301 ccgccgcgtc atgcgtgccg ccaagcctga ctgcgcgagc ctgattcggc gtgtctctcg
      361 cacgcgggat cctcatttca cgatgatcgt tccgaccatt tgcggatgga tcgagcagac
      421 aaagtggtac gtgccgggct gctcgaagcg gaaagtgaac ttgtcattcg tatcgagcgc
      481 cgcggagcgg aatagcccgc tgtcgctgat cacggtgtga ggctcgccat cctggttggt
      541 ccaggtgacc gtcgagccca catcgacggt cagtgaggtg ggcacgaaca tgaagttctt
      601 catgacgacg cctgtggatt ccggtgcggc ggcttgtgct gcgggtgcca tcaacgacac
      661 gagtgtcata cccaataacg ccgacaatga gccggatcgg tgcggattca tgcagactgc
      721 tctccagttt gatgttgtca gggaagtgcg atccggtcag gccaacgtgc ggtcggtgag
      781 ccggagggtg tgcggatgcg cgaccaccga gacgctcgtg accccgagca tggcggggag
      841 ctgtgccgcc gggacgacca ggggtcccgg accggggccg tcgccggccg tcggttgcgg
      901 atacgcggtc gagcgtgcgg tatgaaaggt cacgttgcct tccaccttct gtacgatctg
      961 gtgaatgtgg ccgttgagca ccgtcaccga accgaagcgc cgcagctgac tgaggagttg
     1021 ctccgcatct gcggtacccc aaccccaggg ctgatagatg gtccacagcg gcatgtgcgc
     1081 gaaaacgacg atcggcgtgc tggcagagcg gccgcggagg tcctcggcaa gccaggcgag
     1141 ctgctcatct cccagcgtgc cgagtcctcc gggcttgaac tgcagtacgt tgatgagcgc
     1201 aatgaaatgc acgcccgcat gatcgaaact gtagtagccg cgacgctccg aggctttgcg
     1261 aaaccgattg aagtactcgc tgacggtagg atcggtcaca tcgtgctcac caggcaccgt
     1321 gtgcagctcg gaggcgcgca ggcgcgacag catctgctgc gccagatcaa actcagtcgg
     1381 tctggacaga tgggtgatgt caccggtatg gatgacgagt gcgggctgat cgggcagccc
     1441 attcaccaga tccaccgctc gattgagcgt cgcgtctaca tccggatttg cctccttgtt
     1501 gaagccgatg tgcgtatcgc tgatctgcac gaacagcggc ttgccagccg ggatcgcgcg
     1561 actcgggtcg gttgccgcta cagcgaggtc gatcggcgtg aaaaccccgc ctgccagagc
     1621 gaacaatgtg ccggcaccgc cgagggccat gcacctgagt gctttgcgcc tcccggggag
     1681 gctgatctca tcggtcacgg aagtctcctt tgcagcttca ggagacagga ctgcagcccg
     1741 gcgcgattta ttcccgacgg cgcatcgctg caaggaaccg gatccgagga aatgaatggg
     1801 agcggcgccg atgagcgcaa ttgatccgaa gcccgtgggt gaaccggatg ggtcgggcac
     1861 gagggataaa gccctcgact tctggttcga gttcggcagc aactacagct atctgagtgt
     1921 catgcggatc gaggcggcgg ccgcacgagc cgcggtgcgg atctgctgga agccctttct
     1981 gctcagcccc atcttccgct cactcggctg gagcagctcg cctttcgttc agcagaagga
     2041 gaagggcgag tatgtctgga gggacatggc ccgacagtgc gccaagtacg ggctgccgtg
     2101 gaagcatccg acgagcttcc cgcggcgggc gctgctgccg atgcgcgtgg ctctgtacgg
     2161 cgctcgcgag ccgtggatcg gagagttctg tcggcggatc atgtgcgcaa acttcgccga
     2221 ggatcaggac atcgattcgc cgcggctcgt gggcccgatc ctcgaagagc tcggcgtcgc
     2281 agcgggaacc gttctgcagg gtgcgcagtc cgagaccaac aagcagctgc tgcgcgagca
     2341 aaccgagcgg gctcgtgcgc tcggcatctt tggcgctccc acctttttcg tcggcgagga
     2401 aatgttctgg ggcaacgatc ggctcgagga cgcgctcgcg caatgcagcg cggcgccgcg
     2461 gggctgaagg tcagcacccg gcgaggatca gtcgatcacc gcgagccatg cggtcccgat
     2521 caacagcgtc aggatcgtca gcggagcacc cactcggaag taagcccaga agccgatgct
     2581 cgttccaagc gcctgcgccc gccgggcgac gatgagattg gccaccgagc cgacgagtgt
     2641 gaagtttcca gccagggtgg agctcatcgc aacgaggagc caggcgtgct ggggatgggg
     2701 cagggcaccg agaaagggcc gcagcacgag cacggcgggc acgttgctca cgatgttgga
     2761 caggacagcg gtgaatccaa ccagcaccgg cgcgtgctcg aggtgcagcg cgccggcgag
     2821 cgcatgagct tgcctcgtga gcacgctttc caggcctgcg accacgatga acagaccgac
     2881 gaacatgagc agcagcggcc agtcgagctc ggcatagatg cgctccggct tgaggcgccg
     2941 cgtgagcagc atgagtacgc ccgcgctgat cgcgacagcc gccggcggaa aaccccagaa
     3001 gaaggcgccc accataccgg ccgtgatcac tgctgccttc acgatgaggg cgcggctggc
     3061 gcgcggcgcc ccgaccggcg gtgcagcttg caacacctct gcggtgcgga attccgccgg
     3121 gaaggcgatt gcgatgaccg caatgctcag cagaagggag gcggccgcaa caggagccag
     3181 ggtccccgcg aaaacggcat acgggatgtg ggccaggctg ccgacgatca tgttctgagg
     3241 attgccggta atggtcgcca cgcttccggc attggaggcc atggccactg ccagcaggta
     3301 cggcagtgga tttcggcgca gcgccaccac gatttccagc accagtggcg tgagggcgag
     3361 acagatcgtg tcgttcacga ggaaggctga caggattccc gacaccagga caatccagac
     3421 gagcagcagc agcggacggc gtgcatgcct cgccacggcg gtgctgatca tcgagaacag
     3481 acccgacaac ctcagggtcg caaccacgat catcacgccg agcaacagcg tgacggtgtc
     3541 aaaatcgatg gccgcatagg cctgtcgcaa cgtcaggacg cccgaggcaa tcatgcaggt
     3601 ggcgcctgcc agggctgcgc cggtgcgatc gagacgcaga cccgggaagc ggccccacgc
     3661 gatggccaga taggtcaacg cgaagattac gagcgcggca atctgcgcgg cgtcgtgctt
     3721 catacgccac gacagcaaac gtggcaggcg ttgtcgagcc cccgtgcgtg aaggcggctt
     3781 gtctccggac tgtcacacac tttgtctagt gtagtggttg gtacagccaa gtaacgccga
     3841 ccggtcacgg tcacgaacca acgtccccgg ggagaacaac acaatgatcc tgcgcgcgcc
     3901 tgtcctgaaa agatcgctgc tgctcgccgt tgcggtgagc tctgcaatcc tcgccggcaa
     3961 cacctatgcg cagagcgagg cccacgcgaa cgttccccat gtgaagcatg tgctgctcct
     4021 cagcgtcgac gggttgcacg cccttgacct ctccaactat gtggcctcac acaaggattc
     4081 gacgctcgcg gccctgagcc gtcgtggcgt caccttcacc aacaattcaa cgtcgactcc
     4141 atccgattcc tttccaggac tggcctcgct ggtgacgggt ggctccccca tcacgagcgg
     4201 cctgtggtac gacgatactt acaatcgagc actgtccccg ccggcgcaaa ccgacggtct
     4261 gggcaatccc ggcggcaact gtcccggcaa gatcggcacg aacgtcgcat gggacgaagc
     4321 ggtagacatc gatctcacca agctcgacgg aggcgggggc ctgaatccga acttcctcgt
     4381 gcgcgatccg cgcaacaatt gccacaccat attgcctcat cagtaccttc gcgtgaacac
     4441 gatcttcgag gtcgtgcgtg cgcacggcgg acggacggcg tggaccgaca agcacccctc
     4501 ctacgagtgg accaacggtc cgagcggcaa gggcgtggag gatttctacg gcccggagat
     4561 caattccatc ccagtgcccc tgccgcagat ttccggctgc gcggtcgtac ctttccagga
     4621 cccgacgccg gacgacggct ggacgaacag cttcgatgac atcaagtgct atgactcgct
     4681 acacgtccag gcggtcctca accagatcga cggctacact catgaccgga cgaagaaggt
     4741 tggtgtgccc acgctcttcg gcactaactt ccaggccgtg agcgtcggcg agaagctggc
     4801 gagcgatccg gtcaccgggc tgaaaggagg ctacacggac gtcctcggta cgcccggcga
     4861 ggcgctcgcg ggcgagctgg acttcgtcga tcagtcactc ggccggttcg tcgcggaaat
     4921 gcgccgcgaa ggcattttcg attccacgtt gatcgtcatc agcgccaagc atggccaatc
     4981 gccgatcgac gtgactcgcc gcagaggcat tggtcacggg caacccggcg ccaccattgg
     5041 cgcgccggat gcgttcgaca tctcggatga tggatcgctg atctggctga ccgaccagga
     5101 actcaccgcg agcgtggtcg ccaacctgag ctcgtccgcc aatcaggccg cgctcggcat
     5161 ccaggagatc ttcgccggcg cctcgctgcg caacaagttc gacgatcccg gcagtgatcc
     5221 gcgcacgccg gatatcatcc tgaaggtgaa cacgggcgtg atcttcaccg gcggcacgaa
     5281 gctcgccgag cacggcggct tcaatgaaga cgacgtgcac acggcgctcc tcgtctcctt
     5341 cccggatgcc gagcccgcca gcgtgtccac cgcaaccaca aaccagcaag tggcgccgac
     5401 gattctgcag gcactcggta tcgatccgga cgagctgcag gctgtacagc gcgagcagat
     5461 tgccccgctg cctctgcgac cgcagaggta acggcggtat gggttgagag cacgggcggg
     5521 gcgaggcgtt agtgactcgc gccgtaggcg cgactcttcc agctggcgcc gcgcccccgc
     5581 cagaactgga tggccgaatc gagcgtcgcg cccatgtaaa agagggcaat gagcggcagc
     5641 gtgatggccc agagccgagc gcgtccgtag cgcacgagcg tgggcatgta gctgatcacg
     5701 gccagggcgc atgcagcgac accgcagtca cgctgccagc ctcgtccaag cacggccatg
     5761 gcaaccggcg cccaccacac tattgcaagg ccaagcaggg tcaaagccag cagacaaccc
     5821 gagtggcgca gctgcgtgaa ggccgtgcgc gagaccatgc gccagatgtc cgcggttcca
     5881 ggataggtac ggatggaggt cgccagagcc gagtgtccga ggtagatggg tccggtccgc
     5941 ttcagggctt tggcgagggc cacatcgtcg atcagcgctt ccctgatcgc ggcgagtccc
     6001 ccgatcgcct gcagcgcttc ccgacggatc agcattgttc cgcccgctgc tgcggcggtg
     6061 cgggagcgct gctcgttcac gcgagtgaac ggatagagca tctggaagaa atagacaaac
     6121 gcgggaacga gcatgcgctc ggcaaagctc gtgcaattga gcttcaccat ttcgctgacc
     6181 atctgcgcgc gcggccgctc gagccgcgcg accagcgagg tcagatgtcg cggatcatga
     6241 acgatgtcgg cgtcagtgag gaggagcact ggcgcactcg aagcggccac accctggttc
     6301 acggcccaga gcttgcctga ccagcctggg ggtttggccg gagctcgaag gatcaggaga
     6361 ttcgcggcag cgcccgcgag tgccaccgtg gcgtcagagg agctgtcatc caccagaatg
     6421 acgcgaaagc gacccgcgta gctctgcgcg agcaacgagg ccatcaccgg ttggatggtt
     6481 tgtgcctcgt cgcgcgccgg aacgatgatg tccacatcgg ggggttcggc tggatttgca
     6541 gggggcaact ccggaccgga gacccagaat cgtccgtgag ccaggaacag gtagccccag
     6601 gtcagcaggc acagcacggc cagcaccgat ttaatgtcaa aaatcgcctc cattccgggc
     6661 gtcataatgg gtgttgcggg acggggaccc ttttactatt tactataaaa aagaataact
     6721 taggttcgtc ggaaccgtcc tacagcaaca accggcgttc attgatggcg cgcctcggtc
     6781 ccccggccca tgctgagcac cgtaccagtt cattggcagc gggcagaagg ggctctctgt
     6841 tgaagatctc ggctaacaag acggcgtttt tggggataac gggcctgctt tggacggcgg
     6901 cgacgaccgt cgcacaggca ctcccccccg ggccaggccc agccgtagcg ccgcgctccc
     6961 ctcagttcat gctctacttc agtcgcccca tcggcggcgg cgtggggccg gcgatgtcac
     7021 acccgaccat ggggctcaag atcaaccagg ttcggcagat gccgaataac ggcgaccccg
     7081 aggccaccgg tgaggcgatg cagcatcggg agttgatcaa ctggcagctc gggggtcgtt
     7141 ccgccatacg gctgtcggac atgaggctgc agctcggaca tcgtgtgacc tgggatctct
     7201 cgcgaggctc gttcggcaag acggctccga gcatcaccct ggagccgggg agtctgcggg
     7261 gcgcagcggc gactgcgccg cacactcagc ttggcggccc ccgcagtgca ccatcgatcg
     7321 agcgcattgg tgtcacccgc acgggcgagc gaaacatggc g
//
LOCUS       DASRHN010000220         7168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1554518, whole genome shotgun sequence.
ACCESSION   DASRHN010000220 DASRHN010000000
VERSION     DASRHN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554518"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFB37_08515"
     CDS             <1..337
                     /locus_tag="VFB37_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22535.1"
                     /translation="DTDREVMLVWFREGHPAAPAVASVGDSKTGNAKMQPQPTPHPPA
                     APAAAVPAPQPARPNTGTDPAPASKPVSRPPPAPVMKPATPPANAAPPADKPKPSPPR
                     PGKRSAGAA"
     gene            334..801
                     /gene="mreD"
                     /locus_tag="VFB37_08520"
     CDS             334..801
                     /gene="mreD"
                     /locus_tag="VFB37_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZO22536.1"
                     /translation="MSEHRGRMVITAIVGLILTVLPLPPWLSLVRPAFLVLTVLFWSI
                     SAPRAGGIALGFFAGLMLDVFQGPVLGEHALALCLVTYIAIREHQKIRSKPAFQQSLI
                     VFAALVTYEFVLFAIDGWSGHPITNPLRWTHTVSGAILWAPVAAVLAATSDRR"
     gene            823..2853
                     /gene="mrdA"
                     /locus_tag="VFB37_08525"
     CDS             823..2853
                     /gene="mrdA"
                     /locus_tag="VFB37_08525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZO22537.1"
                     /translation="MARGVHIKDHWSEQRLFDQRALLAGACIALLTIGLLGRLFLLQV
                     MRHDYYTDLSQGNRVRTEPIPAARGLILDRNGEDIAGETPAYQLELIPEEVPDLPGTL
                     KSLVSLGILRADEIDELMRTIRSRRTFDSVPIRLRMSDEDVATFAVRRFEFPGLDIKT
                     RQTRWYPGGALAVHALGYVGAISEPDLEHIDRAAYAGTTLIGKLGVESAYESQLHGTN
                     GFREILVNAQGRSVEHQGIFAANLRTKPPTAGDDVLLSIDLNVQRTAEAALAGHRGAV
                     VALDPNTGGIIALVSLPGFDPNMFGRGITSAEYRALQDDIDRPLFNRALRGTYPPGST
                     VKPVIALAGLTYHMVDPEQKRFCAGSFHLPGSSRIFREYHNEHHGYVDLDDAIAHSCD
                     VYFYGLANTLGVDRIATFMAPFGFGKATGIDIGGEKGGLLPSRAWKAKAFARPADQLW
                     FPGETVNLGIGQGYLLVTPMQLAHYAGVIATRGKIWKPRLVTALRDPLTGKVHQLPPV
                     SEGQVSGVSQEDWDRVIHGMIGVTQRGTAAAIGAHAAYTFAGKTGTAQVFTVAQNEHY
                     NAKLIDERLRDHSWFIAFAPADAPRIALAVLQENGGAGASAAAPIARKVLDAYLLGPD
                     GKLKPAPSDSPGAPPAVPAPKAQEPEAPVAPGPDQKRAGPEPSESRPGQSAG"
     gene            2879..4018
                     /gene="rodA"
                     /locus_tag="VFB37_08530"
     CDS             2879..4018
                     /gene="rodA"
                     /locus_tag="VFB37_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZO22538.1"
                     /translation="MIYDEITSGSRTRRTLTGAARVLFALKLDGPLIIGLALVSVYGL
                     IVLYSASGQNIGTVLRTLGRLALGAAAMGVLAQVNPNFLRRSSPWLYAVGILLLLVVY
                     ATGHIGKGAQRWLDLGIVRFQPSELMKLAVPMTCAWFLHERPLPPSWPALLILSAIIF
                     FPVGLVAIQPDLGTAALIAMAGALVIVMGGLSGRIMAALAALAALGAWFGWSFMHDYQ
                     RKRVLTFLDPQTDPLGAGYHIIQSQIAIGSGGVFGKGWMHGSQAQLDFLPERSTDFIF
                     AVVGEEFGLLGLGLLLLLYLFIVSRALYLSTQTQDTFARLLAGSLALTFFVYVFINAG
                     MVSGLLPVVGVPLPLVSYGGTSVVTLLAGFGILMALYSHRKLVSS"
     gene            4018..5064
                     /gene="mltB"
                     /locus_tag="VFB37_08535"
     CDS             4018..5064
                     /gene="mltB"
                     /locus_tag="VFB37_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HZO22539.1"
                     /translation="MNPARYLLSVGLVVLALVSAQASAAHIAHSAGNHPAHGPKFDLK
                     RAEIVAFVNDVVARDALNRRKVLRLLAKAEPQPKILEIMTRPAEKVMPWWQYHDRFLT
                     EERIAEGVQFYQDHRDILERISAERGIPPEYLVAIIGVETKYGRLTGSYRVLDSLMTL
                     AFDYPPRSEYFRGELEQFLLLISEDGLNPLTTLGSYAGAMGVPQFMPSSYRLYAVDGN
                     TDKKRNLWTDWNDVLASIANYFVAHGWEAGAPVLSEAKLEPDPAFRIDPHNLELNETV
                     DSLNAEGVQIHSDLPGTTPVVLISAEQEDGPAYRVGFRNFYVITRYNHSARYAMAVHD
                     LADALAQRVKSAGS"
     gene            5187..5894
                     /locus_tag="VFB37_08540"
     CDS             5187..5894
                     /locus_tag="VFB37_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZO22540.1"
                     /translation="MPLPAPPARIDSIPDAVPRREPRSAHGNPPFYDVLGRRYVVLAS
                     ADGYIERGVASWYGPTFHGENTSSGERYDMYAMTAAHKTLPLPTYARVTNLRNGRSVV
                     VRINDRGPFVANRLIDLSYTAAAKLDMLREGTTLVEVRAVTPQEPDTLTRTAQSPPPV
                     LYVQAGAFADEGNAKRTLQRLHAAGLGSAFVVAPPDGKAPLYRVRLGPISTVTEFDQL
                     AARLSALGFREARLVTD"
     gene            5916..>7168
                     /locus_tag="VFB37_08545"
     CDS             5916..>7168
                     /locus_tag="VFB37_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZO22541.1"
                     /translation="MQSRRRILAGLAFAGVLLVILAIALVSYAELSALASSSARVQRS
                     YDVLQTLGDLNAALTDTETNQRGYLLTQDPTYLAQYDAGIAQIKLSLTELQRLSSNEP
                     ATQTAIEQLEKLAGAKLDELQELIHATRTGQKPKAMTLFSQGAGKRLMAAFQRDSAEL
                     EAVQRRLLELRREQEHRARLSSEAVLAATVVLSLLLVGAAAQISRRFDERRRLLEQEI
                     AERRRAEKYRESLLISERAARSEAERATRLKDDFVATLSHELRTPLNAIVGWASILGK
                     DRRAETIAQGVEVIERNAKLQAQMIEDLLDMSRIVSGKLLFEMQPTDLAGLVEAAAVS
                     VRPTAEAKGIVMGMNIRAASTVNGDAGRLQQVVWNLLVNAIKFTPRGGRVEVLLAETG
                     GQAELAVTDTGQGIRAEFLPFVFDR"
BASE COUNT         1217 a         2424 c         2262 g         1265 t
ORIGIN      
        1 cgataccgac cgcgaagtga tgctcgtatg gttccgggaa gggcatcccg ccgcaccggc
       61 cgtcgcctcg gtcggggatt cgaagaccgg caatgcgaag atgcaaccgc aaccgacccc
      121 ccatcccccc gcggcacctg cagcggccgt tccggcaccg caaccggccc ggcccaacac
      181 cgggaccgac ccggcaccgg cctcgaagcc tgtctcgcgg cccccgccgg cccccgtcat
      241 gaagcctgcg acgccgccgg ccaatgctgc cccaccggct gacaagccca aaccttctcc
      301 accccgaccg ggcaaacggt ccgcgggggc ggcgtgagcg agcatcgtgg gcgcatggtg
      361 atcaccgcca tcgtcggcct catcctcacg gtgctgccgc tgccgccttg gctgagcctc
      421 gtgcggcccg cctttcttgt gctcacggtg ctcttctggt ccatctcggc gccgcgggcc
      481 ggtggcattg ctctcggatt cttcgccggc ctcatgctgg atgtatttca ggggcccgtg
      541 ctcggagagc acgcgctcgc gctctgtctt gtgacgtaca tcgcaattcg cgagcaccag
      601 aaaatccgct ccaagcccgc ctttcagcaa tcactgattg tgttcgcagc cctggtcacg
      661 tacgaattcg tactgttcgc catcgacggc tggagcggcc acccgatcac caacccgctg
      721 cgctggaccc atacagtgtc cggcgcgata ttgtgggcac ccgtggccgc cgtcctcgcc
      781 gccaccagcg atcggcgctg agcactacag acaccaacgg gcatggcacg cggcgtacac
      841 atcaaggatc actggagcga gcagcgcctc ttcgatcagc gcgcgctgct ggccggcgcc
      901 tgcatcgcgc tgctgacaat cggcttgctc ggccgcctgt tccttctgca ggtgatgcgt
      961 cacgactact acaccgacct ctcacagggc aaccgcgtgc gcaccgagcc gattccggct
     1021 gcacgcgggc tgatactgga tcgcaatgga gaggacattg ccggcgagac gccggcctac
     1081 cagctcgagc tgatcccgga agaggtcccc gacctgcccg gcaccctcaa gtctcttgtc
     1141 tcgctcggca tcctgcgcgc cgatgagatc gatgagttga tgcgcaccat cagatcgcgt
     1201 cggactttcg acagtgtgcc catacggctg cgcatgtcgg atgaggacgt ggcgactttc
     1261 gccgtccgtc gcttcgagtt tccggggctc gacatcaaga cccggcagac gcgctggtac
     1321 ccgggaggtg cgctagccgt acatgccttg gggtatgtcg gcgcaatcag cgagccggat
     1381 ctggagcaca tcgaccgagc cgcctacgcc ggcaccaccc tcatcggcaa gctgggcgtg
     1441 gaaagcgctt atgagtctca gctgcacggc accaacggtt tccgcgagat cctggtgaat
     1501 gcccagggac gctcggtcga gcatcagggg atcttcgccg ccaatctgcg caccaagccg
     1561 cccacggccg gtgatgatgt cctgctgtcg atcgatctga atgtgcagcg cacggccgag
     1621 gcagctctcg ccgggcatcg cggggccgtg gtcgccctcg atccgaatac cggcggcatc
     1681 attgccctcg tcagcctgcc gggattcgat cccaacatgt tcggccgggg tatcacctcg
     1741 gctgaatatc gtgctctgca ggatgacatc gatcggccgt tgttcaatcg cgcgttgcgc
     1801 ggaacctatc ccccgggatc gaccgtcaag ccggtcatcg ccctcgccgg actcacctat
     1861 cacatggtgg atccggagca gaaacgcttc tgcgcggggt ccttccattt gcccggcagc
     1921 tccaggatct tccgcgagta ccacaacgag caccacggct acgtggatct ggacgatgcg
     1981 atcgcccact cctgcgatgt ctatttctac gggctcgcca acaccctcgg cgtggatcgc
     2041 atcgccacat tcatggcgcc gttcggattc ggcaaggcca ccggcatcga cattggcggc
     2101 gagaagggcg gcctgctgcc ctcgcgcgcc tggaaagcga aggccttcgc gcgcccggcc
     2161 gatcagctgt ggtttccggg cgagaccgtc aatctcggaa tcggccaggg gtatctgctc
     2221 gtcacgccca tgcagcttgc gcactatgcg ggtgtgattg ccacgcgcgg caagatctgg
     2281 aagccgcgcc tggtgaccgc tcttcgcgat cccctgaccg gcaaggtgca tcagctgccg
     2341 cccgtatccg agggccaggt cagcggggtc agtcaggaag actgggaccg ggtgattcac
     2401 ggcatgatcg gcgtcaccca gcggggtacg gcggcagcca tcggtgcgca tgccgcctac
     2461 acgttcgccg gcaagaccgg cacggcgcag gtgttcacgg tcgcgcagaa cgagcactac
     2521 aacgcgaagc tcatcgacga gcgcctgcgt gatcattcct ggttcattgc gtttgcgccc
     2581 gcggatgctc cgcggatcgc actggccgtg ctgcaggaga acggcggcgc aggagccagc
     2641 gccgccgcac cgatcgcgcg caaggtgctc gatgcgtatc tgctcggccc cgacggcaag
     2701 ctgaaacctg cgccctccga cagcccgggg gcgccgcccg ccgtgccggc gcccaaagcg
     2761 caggagccgg aagcgccggt cgcaccgggc cccgatcaga agcgggccgg tccggaaccc
     2821 tccgaatcgc ggcccggaca gagcgccggc tgagagcaca ggacatgcgc tcaccaacat
     2881 gatctacgac gaaatcacct ccggatcgcg cacgcggcgc acacttacgg gcgcagcgcg
     2941 agtgctgttc gctctgaagc tcgacggccc gctgatcatc ggcctcgcac tcgtctcggt
     3001 ttacggcctc atcgttctgt acagcgcttc cggtcagaac atcggcaccg ttctgcggac
     3061 cctgggccga ctcgcgctcg gtgcggcggc gatgggagtg cttgcccagg tcaatccaaa
     3121 ctttctgcgc cgcagctcgc cctggctgta tgcggtgggc atcctgctgc tgctcgtcgt
     3181 ctatgccacc ggccacatcg gcaagggagc gcagcgctgg ctcgatctcg gaatcgtgcg
     3241 ctttcaaccc tccgagctca tgaagctggc ggtgcccatg acctgtgcct ggttcctgca
     3301 cgagcgcccg ctgcctccga gctggcccgc gctgctcatt ctctcggcca tcattttctt
     3361 tcccgtagga ctggtggcga tccagccgga tctgggcacc gctgcgttga ttgccatggc
     3421 cggcgcgctg gtcatcgtca tggggggact ctcgggacgc atcatggcgg ccctcgccgc
     3481 gctcgccgcg ctcggcgcct ggttcggctg gagcttcatg cacgactacc agcgcaagcg
     3541 ggtcctcacc tttctcgatc cccagaccga cccgctcggc gccggctatc acatcatcca
     3601 gtcacagatc gcgatcggct cgggcggtgt gtttggcaag ggatggatgc acggcagcca
     3661 ggcacagctg gatttcctgc ccgagcgctc caccgatttc atctttgccg tcgtcggtga
     3721 ggaattcggg ctgctgggcc tcggcctgct gctgctgctg tatctgttca tcgtcagccg
     3781 cgctctgtat ctgtcgacgc agacgcagga cactttcgcg cgactgcttg ccggaagcct
     3841 cgcgctgact ttcttcgtct acgtcttcat caacgcaggc atggtgagtg gcctcctgcc
     3901 agtcgtcggc gtgccgctac cgctcgtcag ctacgggggg acttcggtgg tgaccctgct
     3961 ggcaggcttc ggtattctga tggcgctcta ctctcaccga aagctcgtca gctcgtagtg
     4021 aatccagcac gctacctcct gagcgttggc ctggtcgtgc tcgcacttgt gagtgcgcag
     4081 gcctctgccg cccacatcgc gcactccgcc ggcaaccatc ctgcgcacgg tccgaagttc
     4141 gacctcaagc gcgccgagat cgtcgctttc gtcaacgacg tcgtcgcgcg cgatgcgctg
     4201 aatcggcgca aagtgctgcg cctgctggcg aaggccgagc cgcagccgaa gatcctggag
     4261 atcatgaccc gccctgccga gaaggtgatg ccgtggtggc aataccacga tcgcttcctc
     4321 accgaggagc gcattgcaga gggcgtccag ttctatcagg accaccggga catcctcgag
     4381 cgaatcagcg ccgagcgcgg cataccgcct gagtatctcg tcgccatcat cggggtcgag
     4441 acgaagtatg gccggttgac cggcagctat cgcgtgctcg attccttgat gacgctggcc
     4501 ttcgattacc cgccccgcag cgaatacttc cgcggcgagc tcgagcagtt cctgctgctc
     4561 atttccgagg acgggctgaa tcctctcacc acgctgggct cgtatgccgg cgcaatgggc
     4621 gtcccccagt tcatgccctc cagctatcgg ctctacgcgg tggacggcaa caccgacaag
     4681 aagcgcaatc tctggacgga ctggaacgat gtgctggcga gcatcgccaa ctatttcgtt
     4741 gcacacggct gggaggcggg cgctccggtg ctctccgagg cgaaactgga gccggatccg
     4801 gcctttcgca tcgaccccca caacctcgag ctcaatgaga ccgtggacag cctcaacgcc
     4861 gaaggcgtgc agatccattc ggacctgccg gggacgacac ccgtcgtgct catatccgcg
     4921 gagcaggagg atggtccggc atatcgggtc ggattcagga atttctacgt catcacgcgt
     4981 tacaaccaca gcgcgcgcta cgcaatggcg gtacatgatc tggccgacgc gctcgctcag
     5041 cgcgtgaaga gcgctggttc gtgaagcctg cgagcacgcc ggctatggca tgggcgcggc
     5101 tcgtcattgt tgccgggtgt ggggtgttgg tcgcggcttg cgcggggact cctcgaccat
     5161 tgccttctcc gccttctcaa cccgtattgc cgctgccggc gcccccagcc cgcatcgact
     5221 ccattccgga tgccgttcca aggcgggagc cacgtagcgc gcacggtaat ccgcccttct
     5281 acgacgtcct cggcaggcgc tatgtcgtgc tggcgagcgc tgatggctat atcgagcgcg
     5341 gtgtagcctc ctggtatgga ccaacattcc acggcgagaa tacctccagc ggcgagcgct
     5401 acgacatgta tgccatgacg gctgcacaca agacgctgcc tctgcccacc tacgcacgcg
     5461 tcaccaacct gcgcaacggc cgcagtgtcg tggtcaggat caacgatcgg ggtccgttcg
     5521 tcgccaatcg gctgatcgat ctctcctaca cggcggcggc gaaactggac atgctccgtg
     5581 aggggacgac actcgtcgag gtgcgcgcgg taaccccgca ggagccggac accctgacac
     5641 gcactgcaca gtccccgcct cccgtgctat acgtacaggc cggcgcattt gcggatgagg
     5701 gcaatgccaa gcgaaccctg cagcggctgc acgcggcagg cctgggtagt gccttcgtgg
     5761 ttgcgccgcc cgacggcaag gcgccgctct atcgcgtgcg cctcggaccg atcagcaccg
     5821 tcacagagtt cgatcagctc gccgcgcgcc tgagcgctct cggcttccgg gaggcgcggc
     5881 tggtcacaga ctgaggatca ggcgggaggg ccctgttgca atcacgccgt cgcatcctgg
     5941 ccggcctggc gttcgcaggc gtactgctcg tcattctggc gatcgccctg gtgagctatg
     6001 ccgagctatc ggcgctcgca tcctcaagcg cgcgcgtgca aagatcgtac gacgtgctgc
     6061 agacactcgg ggatctcaac gccgcactca ccgatactga gacgaaccag cgcggctacc
     6121 tgctgacgca agacccgacc tatctggcgc agtacgatgc tggcatcgcg cagatcaagc
     6181 tctctctgac cgagctgcaa cggctgagca gcaatgagcc cgcgacccaa acggcgatcg
     6241 agcagctgga gaagcttgcg ggcgccaagc tggatgagct gcaggagctg attcacgcga
     6301 cccgaacggg ccagaagccc aaggccatga ccctcttctc gcagggtgcg ggcaagcggc
     6361 tgatggccgc cttccagcgc gactcggcgg agctggaggc ggtgcagcgg cgactgctcg
     6421 agctgcgccg agagcaggag caccgggcga gactctcgag cgaggcggtc ctcgccgcca
     6481 ccgtggtctt gagcctgctg ctggtcgggg cagcggcaca gatcagccgc cgtttcgatg
     6541 agcgcagacg actgctcgag caggagattg ccgagcgcag gcgagcggag aagtaccgcg
     6601 agtcgctgct gatcagcgag cgagccgccc gttccgaggc ggagcgcgcc acgcgcctca
     6661 aggacgactt cgtggccacg ctgagccatg agctgcgcac gccgctgaat gcgatcgtgg
     6721 ggtgggcgag catcctgggc aaagaccggc gagccgagac catcgcacaa ggcgttgagg
     6781 tcatcgaacg caatgccaag ctacaggcgc agatgatcga ggatctgctg gatatgagcc
     6841 gcatcgtctc cggcaagctg ctgttcgaga tgcaaccaac agatctggcc gggctggtcg
     6901 aggctgcggc ggtctccgtg cgaccgacgg cggaggccaa agggatcgtg atgggcatga
     6961 acatccgcgc agccagcacg gtcaacggcg atgccggacg actgcagcag gtggtgtgga
     7021 atctgctcgt caatgcgatc aagttcactc cgcgcggggg ccgggtcgaa gtgctgctgg
     7081 cggaaacggg cggccaggcg gagctggcgg tcaccgacac cggacagggc atcagggccg
     7141 agttcctgcc cttcgtgttc gatcgctt
//
LOCUS       DASRHN010000221         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_566591, whole genome shotgun sequence.
ACCESSION   DASRHN010000221 DASRHN010000000
VERSION     DASRHN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566591"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFB37_08550"
     CDS             <1..527
                     /locus_tag="VFB37_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZO22542.1"
                     /translation="AAGVRTPGAETEQLVVFVLHRGTMQDFLPLALEVARLVNEQAGL
                     EVAEVLPVKRIPKTTSGKIQRHLLEVAYADGEFDAERGELAALRTEQRGDQSASRTEI
                     EDKLKVICETALEGKRVDVHDNLFEIGASSLKLIEIHEQIDREYPGLVDLTELFDFPT
                     IVELAGHLESRLAA"
     gene            597..1028
                     /locus_tag="VFB37_08555"
     CDS             597..1028
                     /locus_tag="VFB37_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZO22543.1"
                     /translation="MELHAQSGLPPADRCERRLSECGIRPTAQRVQIAVLLLSGPQHL
                     SAEQILASLRSAGARVSKATVYNTLNLFAAHGLIRQLSIDGSRAWFDSNVDAHYHFHD
                     LSSGALIDVPVPAVEFSRLPAPPAGTEVAGIDLVIRLRKKS"
     gene            1040..1696
                     /locus_tag="VFB37_08560"
     CDS             1040..1696
                     /locus_tag="VFB37_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22544.1"
                     /translation="METGPHPTGTVLWLHGLGADGHDFEPIVPELVRPGERPLRFIFP
                     HAPVRAVTINGGMAMRAWYDILAFDRQTPQDETGIRASDAIVGGLIRRESSRGIPAER
                     IVLAGFSQGGAISLFSGVRYPQRLAGMLGLSCYLLLESRFAAERSTANRTTPVFLAHG
                     SQDPVVDLRAGLLSRELLESAGHPVEWHTYPMAHAVCQQEIADIAAWLRRVLPPPAGA
                     "
     gene            1824..1896
                     /locus_tag="VFB37_08565"
     tRNA            1824..1896
                     /locus_tag="VFB37_08565"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1857..1859,aa:Thr,seq:cgt)
     gene            2228..>3227
                     /locus_tag="VFB37_08570"
     CDS             2228..>3227
                     /locus_tag="VFB37_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO22545.1"
                     /translation="MAPSRDTTQAWQGRAWMLRAGALLLVLAIAAVLWSVMHARPKLI
                     ALADQQSVPLISVMSPGVRSVTTSVTFTGAIAARYDMPIGNDGDTGRIVAVYVEAGDH
                     VQRGQLLAKLNDSVLIPQVNRLEASLEQAKAQAALSEAEYRRALAVQSAGALSAEDIE
                     KRRASAVTDAASVKVAAAQLAEAQARLGRTRILAPIAGTVLTRRAEVGQIASTAGDAL
                     FRIESGDEIEMRGQLAEQDLAQVKVGDAAMVYLTGLPQAFPGRVRLLGAIIDPQTRLG
                     EIRITLKPDAALRPGAFARGTVAVSKASRPVVPQTAVLSDANGTFVFIVNSEGRAER"
BASE COUNT          523 a         1059 c         1091 g          554 t
ORIGIN      
        1 tggcggccgg tgtgcgcacg ccgggcgccg agacggagca gctggtcgtg ttcgttctgc
       61 accgcgggac gatgcaggat ttcctgccgc tggcgctcga ggtagcgcgg ctggtcaacg
      121 agcaggcggg tctggaggtc gccgaggtgc ttccggtcaa gcggattccc aagacgacca
      181 gcggcaagat ccagcggcac ctgctggagg ttgcttatgc cgatggcgag tttgatgccg
      241 agcgcggcga gctggcggcg ctgcgcaccg agcagcgcgg cgaccagtcg gcttcgagga
      301 ccgagatcga ggacaagctc aaagtcatct gcgagacggc actcgagggt aagcgggtgg
      361 atgtgcatga caacctgttc gagatcggcg caagctcgct gaagctcatc gagatccacg
      421 agcagatcga ccgcgagtac ccgggcctgg tggacctgac cgagctgttc gactttccca
      481 ccatcgtgga gctcgccgga cacctggaga gccgtctggc cgcctgaggc gcggcggctt
      541 gcgcccgaag ccgcagattt ccggatttcc gttcgtttgc ggctataatc gccaacatgg
      601 agctgcacgc ccagtcaggt ctcccgccag ccgatcgctg cgagcggcgc ttgagcgagt
      661 gcggcatccg acccaccgcg cagcgcgtgc agatcgccgt cctgttgctc tcgggacccc
      721 aacacctctc ggcggagcag atcctggcga gcttgagatc ggcaggggcg cgggtgtcca
      781 aggccacggt ctacaacacc ctgaacctgt ttgccgcaca cggcctgatt cgccagctct
      841 cgatcgatgg ctcgcgcgcg tggttcgatt ccaacgtgga tgcccactat cacttccacg
      901 atttgagcag cggagctctc atcgatgtgc cggtccccgc agtcgagttc agcaggctgc
      961 cggctcctcc ggcaggcacc gaagtggcgg gcatcgacct cgtgatccgc ctgcggaaga
     1021 agtcatagac gcgatcgaga tcgagaccgg tccgcacccg accgggacgg tgctctggct
     1081 gcacggactg ggggcggacg gccacgactt cgagcccatc gtgcccgagc tcgtgcgacc
     1141 gggcgagcgt ccgctgcgct tcatctttcc ccacgccccc gtaagggccg tgacgatcaa
     1201 tggcggcatg gcgatgcgcg cctggtatga catcctcgcc tttgaccgcc agacgcctca
     1261 ggacgagacc gggatccgcg catccgatgc catcgttggc gggctgatcc gtagagagag
     1321 ctcgcgcggc attcccgccg agcgcatcgt gctcgcagga ttctcccagg gcggtgccat
     1381 ttccctgttc tccggggtcc gttatccgca gcgcctggcc ggcatgctgg gattgtcctg
     1441 ttatctgttg ctcgagtcgc gtttcgcggc cgagcgcagc acggccaacc gcacgacacc
     1501 ggtgtttctc gcccacggca gccaggaccc agtggtggat ctgcgtgccg gcctgttgag
     1561 ccgggagctg ctcgagtcgg ccgggcatcc ggtcgaatgg cacacctatc cgatggccca
     1621 tgcggtgtgt cagcaggaga ttgccgatat cgcggcctgg ctgcgccggg tgctcccgcc
     1681 ccccgccggc gcttgagttt gcgactcatg acctgttccg ggcgggcctg gagggtcaga
     1741 cgtggccgcg gtccgggtaa cccgcggtgc gagctttacg gatgacgggc ctgcctgcag
     1801 cctatagaat cgcgcgtttc ggggccggct tagctcagtt ggtagagcac atcattcgta
     1861 atgatggggt ccacggttcg agtccgtgag ccggcaaatg agcctgcccg caggcggact
     1921 gtcttcgttc cgccactgct cgactgcaac gtttttgtca cgcgagtcgg cccgccgccc
     1981 gggccggtcc gcctggcgcg tcagggcccg gaaatagttt tcagggcgtt aaactttatc
     2041 cgggtcaggc gcttccaacc caggaacaaa cggcacgcgg gcagcggctg aacccgaagg
     2101 aactcaggtg ggtgcaaatc cggaacccaa ggtaggcctc cccaacgggc cagcggaagc
     2161 ggtcacgtgg atcgcccgcg atcgtccgcc gtttgatcgc ctcccgggcg agggatccgc
     2221 gcagccgttg gcaccgtctc gggacacgac gcaggcgtgg cagggccggg cctggatgct
     2281 gcgtgccggg gctcttctgc tcgtgttggc aatcgccgcg gtgctctgga gcgtgatgca
     2341 cgcacgcccg aagctgatcg cactcgccga tcagcagagc gtgccgctca tctccgtcat
     2401 gagtccgggt gtcaggtccg tcacgaccag cgtgaccttc acgggtgcga tcgccgcccg
     2461 ttatgacatg cccatcggca atgacggcga tacgggtcgg atcgtcgccg tctacgtcga
     2521 ggccggcgac cacgtgcagc gaggtcaact gctcgccaag ctgaatgact cggtcctcat
     2581 cccgcaggtc aaccgcctgg aggcgtccct ggagcaagcg aaggcacagg ccgcgctctc
     2641 cgaagctgaa tatcggcgtg cactggcggt gcaatcagcc ggcgccttgt cggcggagga
     2701 catcgaaaag cgccgcgctt cggccgtcac cgacgcggca agcgtcaagg tcgctgccgc
     2761 gcagctcgcg gaggcccagg ctcgcctggg ccgcacccgt atcctggcgc ccatcgcggg
     2821 caccgtgctc acgcggcggg cggaagttgg tcagatcgcc agtaccgccg gagatgccct
     2881 cttccggatc gaaagcggcg atgagatcga gatgcggggc cagctcgcgg agcaggatct
     2941 cgcgcaggtc aaggtgggcg acgcggcgat ggtgtacctc acgggactgc cccaggcctt
     3001 tccgggtcgg gtgcgcctgc tgggcgccat catcgatccg caaacccggc tcggcgaaat
     3061 ccggatcacg ctcaagcctg acgccgcact gcgccccggt gccttcgccc gcggaaccgt
     3121 cgcggtgagt aaggcatcgc gtcccgtcgt gccgcagaca gcggtactca gtgacgcgaa
     3181 cggcacgttc gtgttcatag tcaactccga gggccgggcc gagaggc
//
LOCUS       DASRHN010000222         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1061255, whole genome shotgun sequence.
ACCESSION   DASRHN010000222 DASRHN010000000
VERSION     DASRHN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061255"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFB37_08575"
     CDS             complement(<1..437)
                     /locus_tag="VFB37_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZO22546.1"
                     /translation="MKIVILGAGQVGRTAAYHLSREEANEVTVVDLDEEILRDLQDRL
                     DIRTVAGHASYPTVLEAAGVPEADIIVALTNSDEVNMMACEVAYTLYRTPTKIARIRS
                     REYTSRPELFSDEALSVDVFISPEQLVTEYVERLIQYPGALQVL"
     gene            complement(434..>2193)
                     /locus_tag="VFB37_08580"
     CDS             complement(434..>2193)
                     /locus_tag="VFB37_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZO22547.1"
                     /translation="TRWVPREVQWFVNSNKDEGLRPASLKDGNDPTRRQLIQLYAEYE
                     LACARAGVVDFAELLLRAFELWRDRPELLAHYRRRFRHVLVDEFQDTNSIQYAWMKLL
                     VGPEGFPFVVGDDDQSIYRWRGARVENLQQFRKDFPGAKLYRLEQNYRSTAAILDAAN
                     GLIAHNAGRLGKNLWTSGARGDPIRLYAAFNERDEAEFVMQRIRDWVNRGGQRREIAI
                     LYRSNAQSRVFEETFLSTRIPYKVYGGLRFFERAEIKDALAYLRLVSNRRDDPSFERV
                     VNLPARGIGAKSLDSIRDHAKGTGSSLWEAAGACISSGAIGPKAATALSGFLGLIERL
                     SEQLRGLALHEQVDHILQSSGLIEHYKREKADRGEARVENLEELVSAARGFAPDAADE
                     GQSELDAFLAHAVLESGEGQAEAWEDCVQMMTLHTAKGLEFPLVFLCGMEDGLFPHQR
                     SLNDIAGLEEERRLCYVGMTRAMRELYLTYAEQRRLHGVDSYGQPSRFIREIPDELIE
                     EVRPKVQVARPVAAGRLRYEDPAAPGVRLGGRVRHGRFGEGVILNVEGNGPHARVQVN
                     FESQGTKWLMIQYANLEPL"
BASE COUNT          386 a          731 c          680 g          396 t
ORIGIN      
        1 agcacctgta gcgcacccgg atactggatc agcctctcga cgtattcggt gaccagctgc
       61 tccggactga tgaacacgtc caccgagagc gcctcgtcgc tgaacagctc cgggcggctg
      121 gtgtattccc gggagcggat gcgcgcaatc ttcgtcggcg tgcgatacag ggtgtaggcc
      181 acctcgcacg ccatcatgtt gacttcgtcg ctgttggtga gcgccacgat gatgtcggct
      241 tccggcacac cggcggcctc gagcacggtg ggataggagg cgtgccctgc gacggtgcgg
      301 atgtcgagcc ggtcctgcag atcgcgcagg atttcctcat ccagatcgac gaccgtgacc
      361 tcgttggcct cctcgcggga gagatggtag gccgccgtgc ggccgacctg gccagctccc
      421 agaatgacga tcttcacagc ggctccaggt tggcgtactg gatcatgagc cacttcgttc
      481 cctgactctc gaagtttacc tggacgcgcg cgtggggtcc gttgccttcc acattcagga
      541 tgaccccctc gccgaaacga ccgtgccgca cccgaccgcc caggcgcacc ccgggcgctg
      601 cagggtcctc gtagcgcaga cggccggcgg ccaccggtcg cgccacctga actttcggcc
      661 gcacttcctc gatcagctcg tcgggtattt cgcgtatgaa ccgggaaggc tggccataac
      721 tgtccacgcc atgcagcctg cgctgctcgg cgtaagtcag ataaagctct ctcatcgccc
      781 gagtcatccc gacgtaacac aggcggcgct cctcctccag gccagcgatg tcgttgaggg
      841 agcgttgatg gggaaacaag ccatcctcca tgccgcacag gaacacgagc gggaattcca
      901 ggcctttcgc cgtgtgcagc gtcatcatct gcacgcagtc ctcccaggcc tcggcctgac
      961 cctcaccgga ctcgagcacc gcatgcgcca gaaacgcgtc gagctcggac tggccttcgt
     1021 cggcggcgtc aggggcgaag ccgcgcgccg cgctcaccag ctcctcgagg ttctcgacgc
     1081 gggcctcgcc gcgatcggcc ttctctcgct tgtaatgctc gatgaggcca ctgctctgca
     1141 ggatgtgatc gacctgctca tgcagggcga gaccgcgaag ctgctctgag agccgctcga
     1201 tcaggcccag aaagccgctc agagcggttg ccgccttggg cccgatcgca ccggagctga
     1261 tgcacgctcc agccgcttcc cacagcgagc tgcccgtccc tttcgcatgg tcgcgaatcg
     1321 aatcgagact cttcgcgccg atgccccggg ccggcagatt gacgacccgc tcgaaggaag
     1381 gatcgtcacg acgattcgat acgaggcgca ggtaggccag ggcatccttg atttccgctc
     1441 gctcgaagaa ccgcagtccc ccgtacacct tgtacgggat gcgggtagag agaaaggtct
     1501 cctcgaatac gcgggactgc gcgttggagc ggtagagaat ggcgatctcg cgccgctgtc
     1561 ccccacggtt cacccagtcc cggatccgct gcatgacaaa ctcggcttcg tcgcgctcgt
     1621 tgaaggccgc gtagagacgg atcggatcgc cgcgtgcgcc gctggtccag agattcttgc
     1681 ccagtcggcc ggcgttatgg gcgatcaggc cgttggccgc atccagaatt gctgcagtcg
     1741 agcggtagtt ctgctccagg cggtagagct ttgcgcccgg aaaatccttg cggaactgtt
     1801 gcaggttctc gacgcgggcc ccgcgccagc ggtagatcga ctgatcatcg tcgccgacca
     1861 cgaaaggaaa gccttcgggc ccgaccagca gcttcatcca tgcgtactgg atcgagttgg
     1921 tgtcctggaa ctcatcgacc agcacgtgac ggaagcggcg ccgataatgg gccagcagct
     1981 ccggccgatc ccgccagagc tcgaatgcac gcagcagcag ctccgcgaaa tcgacgactc
     2041 cggcgcgagc gcacgcgagc tcatactcgg cgtacagctg gatgagctgg cgccgcgtgg
     2101 gatcgttgcc atccttgagg ctcgcggggc gcagtccctc atccttgttg gaattgacga
     2161 accactgaac ctcgcgcggc acccagcgtg tct
//
LOCUS       DASRHN010000223        10649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_567298, whole genome shotgun sequence.
ACCESSION   DASRHN010000223 DASRHN010000000
VERSION     DASRHN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567298"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFB37_08585"
     CDS             <1..788
                     /locus_tag="VFB37_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZO22548.1"
                     /translation="LASAYGTAVATTMLLTSCLLFAAMRRVWRWSLPLSLLVAGTFLI
                     VDLAFFAANLLKIAEGGWLPLMLGALVFFVMTTWRSGVDCMHASLEQSTTTSQELLDA
                     LAAGRVPRVHGTAIFLTRLSQRIPRLMIEHVKHMGSLHSHVITLTVLFEQTPRVSEDK
                     RCAVQQIAEGMWHATVRFGFLEIPDLGRSLARLQQLDPAIEIDQAVYFAARDLVARRA
                     GRGLLAHWRLPLFAFLYRNAVKVIDRFSLRSGNVVEIARQIEV"
     gene            complement(798..1742)
                     /locus_tag="VFB37_08590"
     CDS             complement(798..1742)
                     /locus_tag="VFB37_08590"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HZO22549.1"
                     /translation="MSRPESLWPELPYEAWQETCTTLHLWTQIVGKVRLAHTPWLNHG
                     WHVPLYVTSRGLATSPIPYGSRSFEIVFDFNTHVLDIDVNDGSSRRLALRPRTVADFY
                     EQLMRALSELDLATSISERPCEIADATPFGRDRIHSAYDAGYAHRFWRALVQVDRVLK
                     LFRTGFIGKSSPVHFFWGSFDLAVTRFSGRSAPPFTTPVPGLAAAVMREAYSHEVSSA
                     GFWPGNSALPWPAFYSYAYPLPTQFKEQPVQPQGASYNEALGEFVLPYEAVRTASDPE
                     AALLSFLTSTYEAAAIRGSWDRSRLECALGRCGVPRES"
     gene            1816..2373
                     /locus_tag="VFB37_08595"
     CDS             1816..2373
                     /locus_tag="VFB37_08595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZO22550.1"
                     /translation="MAESIDVVAIVGSLRRESFTRRLVQALAAMAPEALRIEIAGIGD
                     LPLYNQDSESSPPAAWVAFRERTRRAQGVLFATPEYNRSIPGVLKNAIDVGSRPYGKS
                     VWAGKPAAVISASPGALGAFGANHHLRQCLVFLDMPAMQQPEAYIGGIDKLLDSAGQI
                     SSASTRALLEQFLRSFEAWVRRFAG"
     gene            2370..3170
                     /locus_tag="VFB37_08600"
     CDS             2370..3170
                     /locus_tag="VFB37_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZO22551.1"
                     /translation="MKVYSWNVNGVRAVVKKGALQSFLKKHQPDILCLQETKAERGQI
                     EIDLPGYHEYWNSALQKGYSGTAILCRRRPVAVVNGFPRELARRFRFADELQRDSAQE
                     GRLITAEFPRFYVVTVYTPNTKDDLSRLSLRHKHWDPAFLSHCRRLEQTKPVIFCGDL
                     NVAHTELDLANPKSNRGRKGFTKEERSGFQNLLDAGFVDTFRLFRQGNGHYSWWSNFS
                     SARERNVGWRIDYILVSGCLRGTVRAADIHVDVMGSDHCPVSITLGRF"
     gene            3220..4533
                     /locus_tag="VFB37_08605"
     CDS             3220..4533
                     /locus_tag="VFB37_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HZO22552.1"
                     /translation="MDDRTGSAETGARTGSRRHRLWKALGPGLITGAADDDPSGIATY
                     SQAGAQFGFDLAWSLLLTYPLMVAIQAVSARIGRTTGHGIAGNLRAHYPGWLVQSMVL
                     LLFAANTLNIGADLGAMAESARLLLPGVPGWLYVLAFGVICIVGQLFIQHGRYVAVLK
                     WLTLSLFAYFATLGVVHVHWVQLAKDLFWPRFARADQFWLTLVAILGTTISPYLFFWQ
                     AAEEVEDTRTSATRSPLRAAPRQGPAALARIRVDTAVGMAFSNLVAMAILVTAAATLH
                     EAGLRDISTAAQAAQALKPLAGSLAFALFALGIIGTGLLSIPVLACSAAYALGEARGW
                     HVGLDRKPGRAKAFYAAIVAATLLGAAGNIAALNPVKALVWVAVLNGIVAVPLMVLVM
                     LLGSRREIMGEFRISGLTRAGGWLATLVMAVAAGALLVSLALPGH"
     gene            complement(4583..5698)
                     /locus_tag="VFB37_08610"
     CDS             complement(4583..5698)
                     /locus_tag="VFB37_08610"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZO22553.1"
                     /translation="MSRLLLVIVAIVLAALVAAVITWLLLSRERRERPQAAAPTNTPP
                     QQPEPPAAADSDGTAPVQGLTPLEIFSQLCAHALGPSPRQKASAAQHESVSRATADAL
                     RDATTQERYAPRRPNMLPRLLSATTDENISRRELSSIIARDPALVGGLLRIANSSYYR
                     ATPEPVESVDRAVALLGTDGLRSLISAVLMQPIFRVGGAHFPRFPETAWEHAYRSASA
                     AVAYAALVEKSDPFAAELLSLVMGLGSIVVFRVVLDQYALDPQLTPDPAVMAALLDAH
                     SASVAQRIGASWELSERMLEALEGQTAAATSHPTPLGRSVHFGRMVGALAVLRINRLL
                     DEQTATASIPATGMDDTQVERMWARLTQDAAKRRAAR"
     gene            5841..7217
                     /locus_tag="VFB37_08615"
     CDS             5841..7217
                     /locus_tag="VFB37_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22554.1"
                     /translation="MRLSTACRPIRSRRSSHTQARRASWVLIASFLPAICSAAASAPP
                     SEFGVDAPELARLGDFAVGVRTLFLVQPDQPDVLAFNPKTGVAPVRERQLTVDLWYPA
                     KPAVSATREIYSATFPSEPPAAPVSFSVPGLAVRDARAAGGGYPLIVVSHGYSNAPAA
                     MTWLTENLASKGYVVAAIRHADPPITYTAGFPQLLLRRPLDIAYVTRTLQSMLATQHL
                     IDPERTALVGYSMGGYGVLTAAGAELDPAGPTVKLVPGGLLRPYAAGGAQRDSVLVKS
                     LRAVVAISPAGGGSLAAWGEAGLREISAPLLLISGDNDQTIDYKTGARAMFDKATGSH
                     RYLLTFRGCGHSIGLNPVPEAMQDRLWDQDWFEDPVWRKDRINEISAHFITAFLDLYV
                     KGDTTRADYLNVPVTSSSAGVWPARDSDAYDAYSPATGGITVWKGFQRNHAAGLELLQ
                     AQAAPASH"
     gene            complement(7220..9373)
                     /locus_tag="VFB37_08620"
     CDS             complement(7220..9373)
                     /locus_tag="VFB37_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZO22555.1"
                     /translation="MNRIDKLISQMTLTEKLGQLTMTAAGYAVTGPVIAGDSTDAIRE
                     GAIGSLLNLVGPEPVREMQRLAVEESRLHIPLLIGLDIIHGHRTLFPIPLAEACVFDP
                     QVWEQTAREAAKDAACDGLAMTFAPMLDISRDPRWGRTAEGPGEDCWVGRHLARAKVR
                     GFQGTDLASGEALAGCAKHFCAYGAVTAGRDYAPVDISQRTLREVYLPPFAEAVAAGV
                     ATIMPAFTDLDGIPMTANTALLRDYLREQLGFDGVIVSDYHAIAELINHGIAADLTEA
                     AVLALKAGVDIDMMANAYRSCLPQALERGQVTVQEIDASVRRVLTLKERLGLFDDPYR
                     RGSRRETAAVLGARRALARSVAVRSLVLLKNDREALPFKKGLARLCLLGPLADAAGEM
                     RGPWWAAGVPEGQVSVLAGLRHGLPGTQIVTAPGVAIEGSDRSGIHHAVSLCADADAI
                     VLCLGESASMSGEAASRACPDLPGEQRALAEAVLDRARAQGKRVTVILFSGRPLSVPW
                     LADRADALIAAWFLGSESGNAIAEVLSGRASPSGRTPISWPRSVGQIPVFYGERPGGR
                     PENPKDHFTSKYLDMPNAPLYPFGHGLTYGRFTLANLRVTPARAAEEDTIEVQVDVTN
                     QGSRAAEETVFVFTRDKVASVTRPVLELRAVGKTVLEPGKSATVKMSFAASELRFPGQ
                     NLQPVFEPGEVEVLVGPCAERSQLLAATLEAYRRS"
     gene            9594..>10649
                     /locus_tag="VFB37_08625"
     CDS             9594..>10649
                     /locus_tag="VFB37_08625"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZO22556.1"
                     /translation="MPGTDLHPTGTPGAGASRAQPGHPPAHTSAADLAALAEEQLLEQ
                     VQRQTFEYFWEGAHPVSGLALDRCQTHGHPTDDKVAVGGSGFGIMAIIVAVERGWITR
                     EAAVDRLGRMVDVLERATCYHGVFPHFLNGRTGATIPFWRKDDGGDLVETSFLCMGLL
                     CARQYFQLQTAAEKSLRGRMTWLWEDVEWEWYTQGGRDVLYWHWSPNNGWAMNHEIRG
                     WNECLITYVLAASSPRHSIEARVYHNGFASGRAFLNGKSYYGIELPLGMPYGGPLFFA
                     HYSFCGLDPRGLQDRYTDYWQLNCRHVAINHAHCVANPNAHKGYGESCWGLTASDDPD
                     GYSVHAPDLDNGTIAPTA"
BASE COUNT         1795 a         3481 c         3475 g         1898 t
ORIGIN      
        1 ggcttgccag cgcctacggg accgccgtgg cgaccacgat gctgctgacc tcatgcctgc
       61 tgtttgcggc gatgcgacgc gtctggcgct ggtcgctgcc gctctcgctg ctcgtcgcgg
      121 gtacgtttct gatcgtggat ctcgccttct tcgccgccaa cctgctgaag atcgccgagg
      181 gcggctggct gccgctgatg ctcggggcgc tggtgttttt cgtcatgacc acgtggcgct
      241 ccggcgttga ctgcatgcac gccagtctcg agcagtccac cacgaccagc caggagctgc
      301 tcgatgctct ggcggccgga agagtgccgc gggttcacgg gaccgcgatc tttctgactc
      361 gcctctcgca acggattccg cggctgatga tcgagcacgt caagcacatg ggctcgctgc
      421 atagccacgt gatcacgttg accgttctgt tcgagcagac gccccgggtg agcgaggaca
      481 agcgatgtgc tgtccaacag atcgccgagg ggatgtggca cgcgacggtg cgcttcggct
      541 tcctggaaat tccggacctc ggccgctcac tggcccgcct gcaacagctc gaccctgcga
      601 tcgaaatcga ccaggcggtc tatttcgcgg cacgcgacct ggtcgcgcgc cgcgccggcc
      661 gcggactcct cgcccactgg cgcctgccgc tgtttgcgtt tctttaccgc aacgccgtca
      721 aggtgatcga tcgattcagc ctgcgctcgg gcaacgtcgt cgagatcgcg cgccagatcg
      781 aggtttgatc cgcacactca ggactcacgg ggcactccgc agcgccccag ggcacactcc
      841 aggcgtgaac gatcccaact cccccttatt gcggccgcct cgtaggtact ggtcagaaac
      901 gacagcaagg cagcctccgg gtcggaggcc gttcgtactg cctcgtaggg cagtacgaac
      961 tcgcccagtg cttcgttgta ggaagcgcct tgcggctgca ccggctgctc tttgaattgt
     1021 gttgggagcg gataggcgta ggaatagaag gccggccagg gcagtgcgct gtttccaggc
     1081 cagaaccctg cactgctcac ctcgtgagag taagcctcgc gcatgactgc ggcggccagc
     1141 ccgggcaccg gggtcgtgaa gggcggcgcc gaccgacccg aaaagcgtgt gaccgccagg
     1201 tcgaagcttc cccagaaaaa atgcaccgga ctgctcttgc cgatgaagcc ggtccggaac
     1261 agcttcagga cgcgatccac ctgcaccagc gcgcgccaga aacggtgcgc gtagcccgca
     1321 tcgtaagccg agtgaatgcg atcgcgaccg aaaggtgtcg cgtcggcgat ttcgcacggc
     1381 cgctcgctga tggacgttgc gaggtccagc tcactgagcg ccctcatcag ctgttcgtag
     1441 aaatccgcca ccgtacgcgg ccgcagtgcc aggcgcctgc tgctgccatc gttcacatcg
     1501 atatcgagga catgagtgtt gaagtcgaaa acgatctcga acgagcggga gccgtaaggg
     1561 atgggcgatg tggccagacc gcgcgaagtg acgtacaggg gcacgtgcca gccatggttg
     1621 agccagggag tgtgtgccag gcgcaccttc cccacgatct gcgtccacag atgcagcgtg
     1681 gtgcaggtct cttgccaggc ttcgtagggt agctcgggcc agagcgattc ggggcggctc
     1741 atcgtgcccg ccatactacg cgccgtctcc gccggatgca ttggggcata attgcggctc
     1801 aagcggaggc aagacatggc ggaatcaatc gatgttgtag cgatagtagg cagtctgcgt
     1861 cgcgagtcct tcacgcgccg gctcgtccag gcacttgccg caatggcgcc cgaggcgctg
     1921 cggatcgaga tcgccgggat cggcgacctg cctctgtata accaggactc ggagagctcg
     1981 cctccggcgg cctgggtggc cttcagagag cgcacccggc gcgcccaggg ggtcctgttc
     2041 gccactccgg aatacaacag gtccattcct ggggtgctca agaatgcaat cgatgtcggc
     2101 tcccggccct acggcaagag cgtctgggcc ggcaagcctg cggcggtcat cagcgcctcg
     2161 cccggggcgc tcggggcatt tggtgccaat catcatctgc gccagtgcct ggtgtttctc
     2221 gacatgccgg ccatgcagca gcccgaggcg tacatcggag gcatcgacaa gctgctcgat
     2281 tcggccggac agatctccag cgccagcacg cgagcgctgc tggagcagtt cctgaggtca
     2341 ttcgaggcct gggtgaggcg gttcgccggg tgaaggtgta ctcgtggaat gtcaacggcg
     2401 tacgcgccgt ggtgaagaag ggagccctgc agtccttcct gaagaaacac caacccgaca
     2461 ttctgtgtct gcaggagacc aaggccgagc gggggcagat cgagatcgat ctgcccggct
     2521 atcacgagta ctggaattcg gcactgcaga aggggtactc gggcacggcc attctgtgcc
     2581 gacgcagacc cgtggcggtg gtgaatgggt ttcccaggga gctggcccgg cggtttcgct
     2641 tcgcggacga gctgcagcgc gacagcgcgc aggagggacg cctgatcacc gccgagtttc
     2701 cacggttcta tgtggtcacg gtatatacac ccaacaccaa ggatgatctg agccgcctga
     2761 gcttgcgtca caagcactgg gatccggcgt tcctgtctca ctgccggcgg ctcgagcaga
     2821 ccaagccggt gattttctgc ggcgacctca atgttgccca caccgagctc gatctggcga
     2881 acccgaaatc caaccgcggc cgcaagggct tcaccaagga ggagcgcagc ggcttccaga
     2941 accttctgga tgcgggcttc gtcgatacct tccgcctgtt caggcagggc aacggccatt
     3001 actcctggtg gagcaacttc tcgagcgcac gcgagcgcaa tgtgggatgg cgcatcgatt
     3061 acatcctggt ttcgggatgt ctgcgcggta cggtccgagc cgccgacatt cacgtggatg
     3121 tcatgggcag cgaccattgc ccggtgagca tcaccctggg caggttctga tcaccgaccc
     3181 gctcgagctt cgggctcgac tcttgcgggc agcccgagca tggatgacag aacgggcagc
     3241 gctgagacgg gcgccagaac aggctcccgc cgccaccgct tgtggaaggc gctcggaccc
     3301 ggcctcatca cgggtgccgc ggatgacgac cccagcggca ttgcgactta ttcccaggcc
     3361 ggcgctcaat tcggattcga tcttgcctgg tcgctgctgc tgacgtatcc gctgatggtc
     3421 gcgattcagg cggtcagcgc gcgaatcggg cgtaccaccg gccacggcat cgccgggaac
     3481 ctgcgcgcgc actaccccgg ctggctggtg cagtcaatgg tgctgctgct ctttgccgct
     3541 aacacgctca acatcggggc tgatctgggt gcgatggccg agtccgcccg tctgctgcta
     3601 ccgggcgtgc ccgggtggct gtatgtgctg gccttcggtg tcatctgcat cgtcgggcag
     3661 ctcttcatac agcacggacg ctacgtggcc gtgctcaagt ggctgacgct ctctctcttc
     3721 gcctacttcg cgaccctcgg tgtcgtgcac gtgcattggg tccagctggc aaaggatctc
     3781 ttctggccgc gtttcgcccg cgcggatcag ttctggctca ctctggtcgc cattctcggc
     3841 acgaccatca gtccatactt gtttttctgg caggcggccg aggaggtcga ggacactcgg
     3901 acgagcgcca cgcgctcacc gctgcgtgcc gctccacggc agggcccggc tgcgctcgcc
     3961 cgcatccgtg tcgacaccgc ggtgggcatg gcattctcca atctcgtggc gatggcgatc
     4021 ctggtgaccg ccgccgccac cctgcatgag gcgggactgc gtgatatctc cacggcggcg
     4081 caagccgcac aggcactgaa gccgctggcc ggatcactgg cctttgcgct gtttgccctg
     4141 gggatcatcg gcaccggtct gctgtcgatt ccggttctgg cttgttctgc ggcctacgca
     4201 ctcggggagg cgcgcggctg gcacgtggga ctcgaccgca agcccggtcg cgccaaggcc
     4261 ttctacgcag ccatcgtggc ggccacgctg ctcggtgcgg cgggcaatat cgccgcgctc
     4321 aatcctgtca aagcgctcgt gtgggtggcg gtgctcaacg gcatcgtcgc ggtgcccctg
     4381 atggtgctgg tgatgctcct cggctcgcgc cgcgagatca tgggagagtt caggatttcc
     4441 gggctcaccc gcgcgggcgg ctggctggct acgctggtga tggccgtggc cgcgggggcg
     4501 ctgctggtct cgctggcgct gccgggccac tagccgtgcg gaaattcctc gcacgcggcg
     4561 ttgaggcgcg cgcctcagcc gctcagcgtg cagctcgcct tttcgccgcg tcctgcgtca
     4621 atcgcgccca catccgctcg acctgagtat cgtccattcc ggtcgcaggg atggacgcgg
     4681 tcgccgtctg ctcatcgagg agacggttga ttcgcagcac cgcaagagca ccgaccatgc
     4741 gaccgaaatg gactgagcgg cccaagggcg tcgggtggct cgtggctgcg gcggtctgac
     4801 cttcgagggc ctccagcatg cgctccgaga gctcccaact cgcgccgata cgctgggcga
     4861 cactggcgga gtgtgcatcg agcagcgctg ccatgacggc cgggtcgggt gtcagttgcg
     4921 gatcgagcgc gtactgatcg agcacgacgc ggaagacgac gatgctgccc agccccatga
     4981 cgaggctgag aagctcggca gcgaaaggat cggacttctc cacgagcgcg gcataggcaa
     5041 cagcggcgct cgcggagcgg taggcatgtt cccaggccgt ctccggaaag cgcgggaagt
     5101 gtgctccgcc gactcgaaag atcggttgca tgagcaccgc ggagatcagc gagcgcaggc
     5161 catcggtgcc cagcagtgcg acggcgcgat cgacgctttc gaccggctcg ggcgtggcgc
     5221 gataatagga gctgttggcg atgcgcagca gtccgcctac gagagccgga tcgcgcgcga
     5281 tgatcgatga gagttcgcgt cgagagatgt tctcgtcggt ggttgcgctc agcagtcgcg
     5341 gtagcatgtt gggtcggcgc ggcgcgtatc gctcctgcgt cgtggcatcc cgcagcgcat
     5401 cggccgtcgc cctcgacacc gattcgtgct gtgcggcaga ggccttttgc cgcgggctcg
     5461 ggcccagagc gtgcgcgcag agttgggaga aaatctccag cggcgtgaga ccctgcacgg
     5521 gtgcagtgcc gtccgagtcg gctgcggcag gcggctccgg ttgctgcgga ggggtgttgg
     5581 tgggggccgc agcttgcggc cgctcgcgtc gctctctgct gagcaggagc caggtgatca
     5641 ccgccgccac cagggccgcc agcacgattg cgacgatgac caacagtagg cggctcacga
     5701 gcggacggtt caggccccgg taacctgtgg ctgacgtcgg ccatttcgcc acaaatgcgc
     5761 tcaatacgcg agcggccggg cacgcgtgct atcctgcccc gccgtcgagt acgcccagac
     5821 gcggcgcgcc cgatccgtac atgagactgt ccactgcatg ccgcccgatc cgctcgcgcc
     5881 ggtcctcgca tacccaggcg cgcagggcga gctgggtgtt gattgcgagc tttctgcccg
     5941 cgatctgcag cgctgccgcc agcgcaccgc cgagcgaatt cggagtcgac gcgcctgagc
     6001 tcgcgcgcct cggtgatttc gccgtgggag tccgaacgct ctttctcgtt cagccggatc
     6061 agccggacgt gctggctttc aatcccaaga cgggagtggc tccagtgcgg gagcggcagc
     6121 tgacggtcga tctgtggtat cccgcaaagc ccgccgtcag cgccacgcgc gaaatctatt
     6181 ccgccacgtt tccatccgag ccgcccgccg ccccggtgag cttttccgtc ccgggtctcg
     6241 ctgttcggga cgctcgcgcc gccggtggag gctatccgct gatcgtcgtc tcccacggat
     6301 atagcaacgc gccggccgcg atgacctggc tgaccgagaa tctcgcctcc aagggctatg
     6361 tggtggccgc gatccggcac gccgatccgc cgatcaccta caccgcggga ttcccgcaac
     6421 tgctcctgcg ccggccgctg gatatcgcgt acgtcacccg caccctgcag agcatgcttg
     6481 ccacgcagca tctcatcgat ccggagcgaa cagcactcgt cgggtactcc atgggcggct
     6541 atggcgtcct caccgcggcg ggcgctgagc tcgaccccgc gggtcccaca gtgaagctgg
     6601 ttccgggtgg gcttctgcgt ccctatgcag cgggcggcgc gcagcgcgat tcggtactgg
     6661 tgaagtcgct gcgcgccgtg gtggccattt cgcccgcagg tggcggttca ttggcggcat
     6721 ggggagaggc aggattgcgc gaaatcagcg cccccttgct actcatctcc ggagacaacg
     6781 accagaccat agattacaag acaggcgcgc gagcgatgtt cgacaaggcc accggctcgc
     6841 atcgctacct tttgaccttc aggggctgtg gccattccat tggcctcaat ccggttcccg
     6901 aagcgatgca ggatcgcctc tgggatcagg actggttcga ggatccggtc tggcgcaagg
     6961 accggatcaa tgagatcagc gcccatttca tcaccgcgtt cctggacctc tacgtcaagg
     7021 gtgataccac gcgagcggac tacctcaacg tgcccgtcac gagctcctcc gccggggtct
     7081 ggccggctcg cgactcggat gcctacgatg cctacagccc tgcgaccggc ggcatcacgg
     7141 tgtggaaggg ttttcagcgc aaccacgccg caggactcga gcttctgcag gcccaggccg
     7201 cgccagcttc gcattgaagt cagctgcgcc ggtacgcctc gagagtcgca gccagaagct
     7261 gtgatcgctc cgcgcaaggt ccgacgagca cctcgacctc gcctggctcg aacacaggct
     7321 gcaggttctg cccgggaaag cgcagctcgg atgcggcgaa gctcattttg acggtggctg
     7381 actttccggg ctcgaggact gtcttgccga ccgcgcgcaa ctccagaacg ggacgcgtga
     7441 cactcgccac cttgtcgcga gtgaagacaa acaccgtctc ctcggcggca cggctgccct
     7501 gattcgttac atcgacctgc acctctatcg tgtcctcttc ggcggcgcga gccggcgtga
     7561 cgcgcagatt ggccagcgtg aagcgcccat aggtgagtcc atgcccgaag ggataaaggg
     7621 gcgcattcgg catgtcgaga tatttgctgg tgaaatggtc cttggggttc tccgggcggc
     7681 cacccgggcg ctcgccataa aatacgggga tctgacccac ggaccgcggc cacgagatcg
     7741 gcgtgcggcc gcttggcgag gcccgtccgc tgaggacctc cgcaatcgca ttgcccgact
     7801 cactgcccag aaaccacgcg gcaatcagcg cgtcggcccg atcggcaagc cagggaacac
     7861 tcagaggccg tcccgagaaa agaatcaccg tgacccgctt gccctgcgcg cgtgcccggt
     7921 ccaggaccgc ttcggccagg gcacgctgct cacccggcaa atcggggcag gcccggctgg
     7981 ctgcctcgcc gctcatggag gccgactccc ccagacagag cacgatggca tccgcatcag
     8041 cgcagagcga gaccgcatgg tgtatgccgc tgcggtcgga tccttcgatc gcgacgcccg
     8101 gtgcggtcac gatctgggtg ccgggcaatc cgtggcgcag gccagccagg acgctcacct
     8161 ggccctccgg cacaccggcg gcccaccatg gcccgcgcat ctcgccggcg gcatccgcca
     8221 gcggacccag cagacacaag cgtgccagcc ctttcttgaa cggcagcgcc tcgcgatcgt
     8281 tcttcaatag aacaagggag cgcaccgcga ccgagcgcgc cagcgcgcgc cgggcaccca
     8341 gcaccgctgc cgtctcgcgt cgcgatccac ggcgatacgg atcatcgaat agaccaagac
     8401 gctccttgag cgtcagtaca cggcgtacgg aggcatcgat ctcctgcacg gtcacctgac
     8461 cgcgctcgag agcttgcggc aagcagctcc ggtaggcatt ggccatcatg tcgatgtcca
     8521 cgccggcttt gagcgccagc accgcggcct cggtcagatc cgctgcgata ccatgattga
     8581 tgagctcggc gatggcgtga tagtcactga cgatcacacc gtcaaagccc agttgctcac
     8641 gcagataatc acgcaacaga gccgtgttgg cggtcatggg aatgccgtcg agatccgtga
     8701 aggcgggcat gatggtcgcg acgccggccg cgactgcctc ggcaaaggga ggcaggtata
     8761 cttcccgcaa cgttcgctgc gagatgtcga ccggggcgta gtcacgtccg gccgtgacgg
     8821 ctccgtaggc gcagaaatgc ttggcacagc ccgccagagc ctcgccggat gccaggtctg
     8881 tgccctgaaa gccgcggacc ttggcacgtg cgagatgccg tcccacccag cagtcctcac
     8941 cgggaccctc ggcagtgcgc ccccagcgcg gatcacggga aatatccagc atgggcgcga
     9001 aagtcatggc aaggccgtcg caagcggcgt cctttgccgc ttcacgggcg gtctgttccc
     9061 acacctgcgg atcgaatacg caggcctcgg ccagcggtat cggaaagagc gtacggtgcc
     9121 catggatgat gtcgagtccg atcagcaagg gaatgtgcag ccgggattcc tcgaccgcca
     9181 ggcgttgcat ttcccgcacc ggctccgggc caaccaggtt gagcagactg ccaatggcgc
     9241 cctcgcggat cgcatccgtc gaatccccag cgatcaccgg gccggtcacg gcatagcccg
     9301 cggctgtcat ggtgagttga cccagttttt ccgtcagggt catctggctg atcagtttgt
     9361 cgatccggtt catcgaaaat ccgcccttga acacgctagg gtgatgatag aggggtgggt
     9421 ggaaatcgcc agcggatcca accggggggc ggcccgcgct cagccgcgag ccctcgctgg
     9481 aagccttggc tgcgcagggt ggcctgtgat ttccaacatg ccaattgcgc cgcaaccggt
     9541 tcagaattcc cgggcgcccc gcggcgaccg tgtctcatgc ctcaggagag cccatgcccg
     9601 gaaccgatct gcacccgaca ggcacccccg gcgccggtgc gagccgcgct cagccggggc
     9661 atccgccggc gcacacgagc gctgccgatc tcgccgcgct ggccgaggag cagctgctgg
     9721 agcaggtaca gcggcagacg ttcgaatact tctgggaggg cgctcatccg gtgagtgggc
     9781 tggccctcga tcgatgccag acgcatggcc atcctacgga cgacaaggtc gcagttggag
     9841 ggtcgggttt cgggatcatg gcgatcatcg ttgccgtcga gcgcggctgg atcacgcgcg
     9901 aggccgcggt ggatcggctg gggcgcatgg tcgatgtgct cgagcgtgct acctgctatc
     9961 acggcgtttt cccacacttc ctcaacggac gcacgggcgc caccattccg ttctggcgca
    10021 aggacgatgg cggagacctc gtcgagacct cgtttctgtg catgggattg ctgtgtgcgc
    10081 ggcagtactt tcagctccag accgcggccg agaaatcgct ccgtggccgc atgacctggc
    10141 tatgggaaga cgtcgagtgg gagtggtaca cgcagggtgg ccgggacgtt ctctattggc
    10201 actggagccc gaacaacggc tgggcgatga atcatgagat ccgcggctgg aacgaatgcc
    10261 tgatcaccta tgtgctggcc gcatcctcgc cgcgccactc gatcgaagcg cgcgtgtatc
    10321 acaatggctt tgcctccggg cgcgcttttc tcaacggcaa atcctattac gggatcgagc
    10381 tccctctcgg gatgccttac ggcggcccgc tcttcttcgc ccactactcc ttctgcggcc
    10441 tcgatccgcg cggcctgcag gatcggtaca cggactactg gcagctcaat tgccggcacg
    10501 tcgcgatcaa tcatgcccat tgcgtcgcca accccaacgc ccacaagggt tatggcgagt
    10561 catgctgggg cctgacggcg agcgatgatc ccgatgggta ttcggtccat gcgccggatc
    10621 tggataacgg cacgatcgcc ccgaccgca
//
LOCUS       DASRHN010000224         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1397391, whole genome shotgun sequence.
ACCESSION   DASRHN010000224 DASRHN010000000
VERSION     DASRHN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397391"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VFB37_08630"
     CDS             complement(<1..1454)
                     /locus_tag="VFB37_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZO22557.1"
                     /translation="MSVRTWWLGSWIGRALSAVVAIKLVLAAAIWLLGDRQSLELLDR
                     IANLALLIVAVLAFHQWTLQARGQLLWWVRRRLILSYVLVGAVPILLLSAFALLGFLL
                     LFFDVSSYLVRNRLTAVTDQASTLARTTLVELDRSPFETRPEVLSRRHAVLETRYPGI
                     ELSMVPTTGVPRCGLAASKTLPPQPAGTLPRWVSCRGFAGMLLFASEPGTAGPLKLVA
                     RGAALPNRANPDYAVLVDLPVDQAGTAASLEGTGIQLGELTVVSGENTASPLAAQPKV
                     IVPDPRTSSRSLFNTATFLTYTDWQTGLTGRAALSMSVQVGTLYRWLGGEANRSADVT
                     FSRILLFTLVGIGGLLFVIEVIALANGLALARSITDAVDELFRGTERVKVGDYSKPIP
                     QRTEDQLGQLASSFNDMTRSIKHAMEERAEKQRLEEELRIARDIQMSLLPLGPYSIPG
                     VVLSGVCAPAREVGGDYYDYLPLEDGRLGLLIADV"
     gene            complement(1487..1816)
                     /gene="cutA"
                     /locus_tag="VFB37_08635"
     CDS             complement(1487..1816)
                     /gene="cutA"
                     /locus_tag="VFB37_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZO22558.1"
                     /translation="MEKTCVVVLTTVGSGDDPNALAATLVTERLAACVNVHGVMNSTY
                     RWKAAVESAYEHQLTIKTTADCVPALQARLHQLHGYEVPEFLVMPVSGGSDRYLDWIR
                     ESTRKAG"
BASE COUNT          378 a          652 c          641 g          338 t
ORIGIN      
        1 acatcggcaa tcagcaaccc caaccggccg tcttccagcg gcagatagtc gtagtagtcg
       61 ccgccgacct ctcgcgcggg tgcgcagacc cccgacaaca ccacccccgg aatgctgtag
      121 gggcctaggg gcagcagcga catctggatg tcgcgcgcga tccggagctc ctcttcgagg
      181 cgctgcttct ccgcgcgctc ttccatcgca tgcttgatgc ttctcgtcat gtcgttgaag
      241 gacgacgcga gctggccgag ctgatcctct gtgcgttgcg gaatgggctt cgagtagtcc
      301 cccaccttca cacgctcggt gccgcggaag agctcgtcca cggcgtcggt gatggaccgc
      361 gccagggcca ggccgttggc gagggcgatc acttcgatca cgaagagcag acccccgatc
      421 cccaccaggg tgaacaggag gatgcggctg aaggtgacgt ccgcagaccg attggcctca
      481 ccgccaagcc accggtagag ggtgccgacc tgcacgctca tcgagagcgc cgctcgtccg
      541 gtaagtcctg tctgccagtc cgtgtaggtc agaaacgtag cggtgttgaa cagcgatcgc
      601 gaagacgtcc tgggatcggg gactatcacc ttcggctggg ccgcgagggg tgacgccgta
      661 ttttctccgg acaccaccgt aagttcgccg agctggatgc cggtgccctc gagagaggct
      721 gccgtgcccg cttgatcgac cggcagatcg acgagcaccg catagtcggg attcgctcga
      781 ttcggcagtg ccgctcctcg tgcgacgagc ttcagcggtc cagccgtgcc cggctcggag
      841 gcaaacaaca gcatgccggc gaagccacgg caactcaccc agcgcggcag cgtgccggca
      901 ggctgcggcg gcaacgtctt ggaggcggcc agcccgcagc gcggtacgcc ggtggtgggc
      961 accatcgaca gctcgatccc tggatatcgg gtctccagca cggcatgacg gcgtgagagc
     1021 acctccggcc gcgtctcgaa cggcgatcga tccagctcga caagggtcgt tcgcgccagc
     1081 gtgctcgcct ggtcagtgac cgccgtcaag cggttccgca ccagatacga actgacatca
     1141 aagaagagca ggagaaagcc gagcagcgcg aacgcactca gcagcagtat cgggaccgcg
     1201 ccgacgagga cgtaggacag aatgaggcgc cgccgcaccc accacagcag ctggccgcgc
     1261 gcctggagcg tccattggtg aaacgccagg acggcaacga tgagcagcgc gaggttggcg
     1321 atgcgatcga gcagctcgag gctttgtcga tctccgagca gccagatggc ggccgccagc
     1381 acgagcttga tcgccacgac ggccgagaga gcccggccaa tccaggagcc gagccaccat
     1441 gtgcgcacgc tcatcgatca gtcatctcga ggtgcgccgc atgatatcac ccggctttcc
     1501 gggtggattc gcggatccaa tcgaggtacc ggtcgcttcc cccgctgacg ggcatcacca
     1561 gaaactccgg aacctcatat ccatgcagct gatgaagccg cgcctggagt gccgggacgc
     1621 agtcggcggt cgttttgatc gtcagctggt gctcgtacgc agactcgacc gcggctttcc
     1681 atcgatacgt cgagttcatc acgccgtgca cgttgacgca ggccgcgaga cgttcggtga
     1741 cgagcgtcgc agctagcgcg ttgggatcgt caccgctgcc caccgtagtg aggacgacga
     1801 cgcaggtttt ctccatggga ataccctttt tctatttgta ctttcccgcg aaacgtggta
     1861 cacgttttgg tgcgccacat gccacgccat caggacaaca ccaagccagg caaaggattg
     1921 cagcgccgcg ggcaacggct gctggcgggg ctgttcggct tcatcgtctg cgtcctgctc
     1981 gtcaatgcac tggtcggcga tcagggcct
//
LOCUS       DASRHN010000225        16183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2217552, whole genome shotgun sequence.
ACCESSION   DASRHN010000225 DASRHN010000000
VERSION     DASRHN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16183
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217552"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFB37_08640"
     CDS             <1..273
                     /locus_tag="VFB37_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22559.1"
                     /translation="IGLLSVNRAGLEKLKTVLGKLQQRADFSALDMPALINGLISSGA
                     AIEVLYVHGHWRGVNDLEDFGRAVEFAHGQTPLGGARGAAGVDEAT"
     gene            270..1454
                     /gene="aepY"
                     /locus_tag="VFB37_08645"
     CDS             270..1454
                     /gene="aepY"
                     /locus_tag="VFB37_08645"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR03297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HZO22560.1"
                     /translation="MIEAAQFVEAAREYGFEWYAGVPCSHLTPFINYVLQDRSLHYLS
                     MTNEGDAVALIAGIALGARAEGHGRGVAMMQNSGLGNAVSPLTSLTWTFHLPQLLIVT
                     WRGQPGSHDEPQHALMGPITPTLLETMQIPWELFPRERDEIDPALRRAASHMEETGRP
                     YALLMQKGSVAACELKPGSQQLQARGAKAQPIVRLRGLATESRASRNDALREVIARTP
                     SSRCAVLATTGYCGRELYAIEDRPNQLYMVGSMGCVLPLALGLALARPDLRVLAVDGD
                     GAALMRMGAFATAGAYGPPNFSHLLLDNGVHDSTGGQATVSTHVSFAGIAASCGYASA
                     LQTDDLGEIAEWLDGSVLEGSRFACLLTRKGTPDSLPRPSVSPRELKLRWMQHFAPAG
                     VR"
     gene            1454..2536
                     /locus_tag="VFB37_08650"
     CDS             1454..2536
                     /locus_tag="VFB37_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="HZO22561.1"
                     /translation="MQLLLNPGPVTLSERVRRSLLQPDLCHRESEFFDLQDTARELLL
                     GTYELAPKTWTAVLMTASGTGAVESMVASLVPANGRVLVIENGVYGERIAQICKQYGI
                     AHDRIRGDWMQPPDLAEVATRLDSARGDAAFTHIAVVHHETTTGRLNDLPSLADVCRA
                     RRVRLLVDAVSSFGAEAIDFADETVAAVAATANKCLHGVPGVAFVIARRESLATAANR
                     TYYLDLARLARAQDQRNTPFTPAVHACYAMVEALREYAEEGGRAARFRRYAALAEQVR
                     AGLAALAIRAVLPPEESSVVLRAYTLPAHMQYPALHDALKAGGFVIYAGQGELAKILF
                     RISTMGNLNSADIERLLTCFARLPAN"
     gene            2509..3807
                     /locus_tag="VFB37_08655"
     CDS             2509..3807
                     /locus_tag="VFB37_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZO22562.1"
                     /translation="MLRPFASKLIGGVALALLLGTRALAQTQVPAPDASGAQPSAPPP
                     VESSWYNRLPFIPVPDIGSDPDSGTTVGILPVWLKTDDQHAIRRIIAPDFVYNPFFGY
                     GIDARLYAYDSEDEQWSIEGGVKERVERDFDAEWQRGRLREGPWSYTASLIYSRDGTP
                     RFFGIGNNSPAIAETNYTNSQELAQIQVGHNFSPVWQLLYTLRVRTVHVLPGTLARIA
                     SIQSRFADILGIGTNSEVLNRISLVYDTRDNLTAPRRGMQWVIYGGLATRTVVHDNTD
                     AVYDETGIDGRSYLPIAPQTILATHVALRYLPSVRRLPFWALSSIGGGESVIGGEQPL
                     RGYGAGRFYDRNAFSASAELRHTVWSFDAMSTHVDIELTPFVDVGQVFARTGTWPIQD
                     LHKVGGIGFRGVARPSVVGFVDVGYGNEGVAVFTGINYPF"
     gene            complement(3848..4975)
                     /locus_tag="VFB37_08660"
     CDS             complement(3848..4975)
                     /locus_tag="VFB37_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO22563.1"
                     /translation="MNEELSLFREAIARFIAAEMTPNDAAWRKQQHVGPEIWRRAGEL
                     GLLCADVPAQYGGVGGSFVHEAVVHEELARTGMSGFGAGVHSICAHYLLNHGLEEQKQ
                     RWLPRMARGELIGAIAMTEPGAGSDLQAIRTRAQRQGDEYLVSGSKLFITNGYLAGLL
                     ALAVKTDPAQRAKGISILMVETRDLPGYRVGRILDKMGMKSQDTAELFFEDVRVPASN
                     LLGGEPGRGFAQMMSDLPYERMIVALAAVGAMEGAIEATLHYVKERKAFGHPVAEFQN
                     ARFKLAEIATVARIARAFVDDCVGKVVEGTLDTATASMAKWWTTDMQQHVLDECVQLH
                     GGYGYMNEYLVCRMFADARVQRIYGGTNEIMKELIARSLLR"
     regulatory      5179..5365
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5512..7431
                     /locus_tag="VFB37_08665"
     CDS             5512..7431
                     /locus_tag="VFB37_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22564.1"
                     /translation="MRESVLLSMLAATALWTPGFSRATDADVAGAPAADAETPDSVVV
                     TATFIPTPEQEIASSMTVITAQDIETHRQQSLPQVLRDVPGLNVVQPGGPGGYTSLFM
                     RGTNSNHTKVLVDGIDVTDPSSSGSTFDFGQFLSQDIERVEVLRGPQSGLYGADAIGG
                     VINIITRSGAGPAQVWGSAEAGSFDTFSQAAGLSGAAEQFHYSANLEHLHAGATPVTP
                     LDLLLPGEKRHDDSYDNLTASTKLGLDVTEHFDLGLVARYTDSHLRFTGDDEFAFPSF
                     PQARQSASNTSEYYARAFAHGVAADGFIDQTLGVGFTRNRSVNFDPEIPATLDIGERA
                     KVDWRGALKFSDAQTLVLGAEHEHDEISQPVSAGVAISAGYGELQSQLGEHLFSALNL
                     RYDANSQFGGKATYRLAPAWLISETGTKLKASIGSGFKAPTLGELYQNFPQFFFFGNP
                     NLKPETSIGYDLGFEQSVAQETVRFGATYFRNRIRNLIDIAASGATYANFGRATTQGV
                     ESFLSWSPVKSLLFRLDYTYTEATDDIRDQELLLRPRHKGSLDVRWQPLQPLSLDLNV
                     LAVGTYIDGNRDFSIPRLQAPGYTLANAAVNLAITPHLTAYARVDNIFDRHYESPVGF
                     LQPSLGAFVGLSVKL"
     gene            complement(7436..8977)
                     /locus_tag="VFB37_08670"
     CDS             complement(7436..8977)
                     /locus_tag="VFB37_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO22565.1"
                     /translation="MTRSSSTRRTPSARVPRCGPGPRQRNRAAPRNRPPAHPEHMHRT
                     LPMLVCAAVAACSFAPRYSRPPVETPPPRWKESVDWKLAQPADAQSRGPWWSLFGDPT
                     LDTLEARVTGANQNIKAAFARLQEARAQTRVARAAYFPTLTVGPSATRSRVSLNSPLY
                     TNTKPAIGNDFVLDADLSYEIDVWGRVRNSVASARATEQATAADLATLDLSTHAELAA
                     DYFTLRGEDTQLELLDRTVEDYRQALELTEHLYNGGAAALADVDQSKAQLESARTQAT
                     DARLRRAQTEHAIAVLIGESPSSFRLDPQPLKPEVLPPVLDPGLPSALLERRPDVAAA
                     ERRVASANAQIGVARAAYFPVFDLFGRVGFESTQASNWITAPSRLWSIGPSALLTAFD
                     GGRYLAQSAAAHAAYDEQVADYRNTVLTAYQEVEDNLAALRDLESESTSQAAAVAATT
                     GALTQTQYQYKGGVVTYLQVVITENAALSAELTASDIQVRRLTAAVLLIKALGGGWQP
                     RVSGT"
     gene            complement(8862..10064)
                     /locus_tag="VFB37_08675"
     CDS             complement(8862..10064)
                     /locus_tag="VFB37_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO22566.1"
                     /translation="MTEPVPPSADEITVHRVKRYAWIALVVAVLLAIWGVASRVHSRS
                     SLGKQTANEAVPVVISSHPEGSPASEELVLPGNVQAFIEAPIYARTSGYLRVWYTDIG
                     TPVSKGQLLAEIDTPEVDQQLRQSRADLATARANAQLAQSTNTRWQGLLANHAVSQQD
                     ADTRAADAAATRASAASAEANEARLEQLETFKRVVAPFDGVVTGRNTDIGALINAGQS
                     SGSWLFRVADTKKLRIYVDVPESYAARMTVGLQAQLHFNEHPGEDYPATLVRTAQALD
                     PTLRTLQVELQVDNSEGELFPGAYAEVHFKLPGSSSTVRVPATALVFRAQGLQVATVT
                     DGNHVHMKGILQGRDFGTSVEVLSGLTANDEIIVNPPDSLSEGSTVRTRPPPAQQGSA
                     PKPAARAP"
     gene            complement(10061..13243)
                     /locus_tag="VFB37_08680"
     CDS             complement(10061..13243)
                     /locus_tag="VFB37_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO22567.1"
                     /translation="MWIVKVALQRPYTFIVLAILIFLMGTFAILRTAVDIFPSIKIPV
                     VAAIWNYAGLQPIEMANRIVLGTERGAQTTVNNIEHTESQSLNGISVVKYFFQPGADE
                     VLSYAQITGVSQTSLRNLPPGATPPFILAYDASTVPIIQLALSSESLSEAQIFDLGNN
                     IIRTALATIPGAAIPWPYGGRQRQVQVDLDLPALRARGLSGSDVTAAITQQNLILPAG
                     TQKIGDLEYYIGLNASPQKIEELNDLPISSTANGNVVYVRDVAHVRDGSPPQTNIARL
                     GGRRASLMTVLKLGKASTLDVINAIKQKLPQIREMVPPQLRIEPIGDQSIFVRAAISG
                     VVREATIAAALTGLMILLFLGSWRSTIIIFISIPLSILASIACLAALGETLNIMTLGG
                     LALAVGILVDDATVTIENINSHLEEGEEVERSILHGAQQIALPALVSTLAICIVFVPM
                     FMLAGIAKFLFVPLAEAVVFAMLASYLLSRTLVPTLSKYWLTTHEHERTGRGGWLARF
                     QAGFERGFTRTRDRYHALMEMALHGGLRFAGIFLAAMVLSAILAFPFGTYLPGLGQDF
                     FPAVDSGQIKLHFRARTGMRIEETAALCDRVEAGIRRVIPAGELESIVDNIGLPYSGI
                     NLSYSTSAPVGPSDADIFVALKKSHHATADYMRTLRSVLNAGFPSTSFSFPPADIVSQ
                     TLNFGLPSPIDVQVVGNDLQSNRTYANLLLQRLRAVPGAVDLHIQQAFDYPTLNVDVD
                     RTKARQLGLTQQNVASNMLVSLSGSFQTQPSFWIDPHSGTQYNVVAQTPQPQLETLNS
                     LATTPLTNGGGGAGQLLANVATFHRSASPAVVSHYDATPVLDIYGSVQDTDLGYISTQ
                     VNRIVEATKGQVPRGSHVVVRGQVQTMNASFHGLLLGLLAAVVLVYLLIVVNFQSWLD
                     PLVIITALPAALAGIVWMLFLTHTTVSVPALTGAIMCMGVATANSVLVVSFARERMNA
                     GDDAFTAASKAGFTRFRPVLMTALAMIIGMIPMALSLGEGGEQNAPLGRAVIGGLVFA
                     TIATLFFVPTVFTLIHGRTAQREAADGSPA"
     gene            complement(13440..14321)
                     /locus_tag="VFB37_08685"
     CDS             complement(13440..14321)
                     /locus_tag="VFB37_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZO22568.1"
                     /translation="MGALLLTGGCMVIEAAGGWIAGSLALLADSGHMLTDCASLAMSY
                     AAVRAGTRPATASMSYGHHRWQVLAAFVNGLALLLLAVWIAAEAVQRLRTGTVVQGQV
                     VAGVAVAGLLVNVLAFVVLSRGEANLNVRGALAHVMGDILGSLAALIAGVVIVLTGWM
                     PADPLLSGVVALIMARSGWRISRESAHILLEGAPPDCDGEQIEHALSTAIPAVAGVHH
                     LHVWSLTDERPVVTLHAVLRENADRDRVLVDIQTTLRERFGVQHATVQIELTECEDED
                     CMSASAVGPASATGPVL"
     gene            complement(14559..>16183)
                     /locus_tag="VFB37_08690"
     CDS             complement(14559..>16183)
                     /locus_tag="VFB37_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22569.1"
                     /translation="PVINFVSRTPATQTFAGTTEQIGGDEGLFSSFNAISGTLGTWDY
                     LADYSHRQSSGQRRNGDYELNSGDLQVGFRPDTQQTLTFAVHAYSLESGLAGLMSGAQ
                     FRANSDQATTPDDRLWTDRYTAVLSYDNALTEHDLLTQKLWTGYTDLITRADTYNRID
                     DSATGGMLTGQRFHYTGLDGRLLHRWGSGNALTVGYTAYWSRSPYTESIDPNPLADRT
                     DESGLPFYRDERETHYGAIFAENVFRFPWFHLVTSARLDHEELSTHESVAPHPQLVDA
                     TYSKSVPLFGVGIGNDFGRGNETYLNVSQGFRPLRYLDIASPFSNFSPTNNPDPTRYL
                     TYEAGVHGWPSAGLYYDVSVFQVNVRNRIESQQITQTETIDVNTGDTRSRGAELEAAY
                     DVLRLWPQAESSQHLTLFANASLLNARFTSSIIPGQTGKRPAYAPDYVLKAGATLREE
                     GHYKISLIADSVASQFFQDSDLAVGSTPARIPMYAVVDLSGEYVIGGHLRLLGGIANL
                     GDRHYYSRVFLFGGQLEPGRDRTFHAGLAYDF"
BASE COUNT         2831 a         5129 c         5369 g         2854 t
ORIGIN      
        1 atcgggctgc tgagtgtgaa ccgtgcggga ttggaaaagc tcaagaccgt actcgggaag
       61 ttgcagcagc gcgcggattt cagcgcactg gacatgccgg cgctgatcaa cggcctcatc
      121 agcagcggag cagccatcga ggtcctgtat gtgcacggtc actggcgcgg ggtcaatgac
      181 ctggaggact tcggccgtgc ggtcgagttt gcgcatggac aaacacctct tggcggtgcc
      241 cgcggtgcgg caggggtgga tgaggcgaca tgatcgaggc cgctcaattc gtggaggcgg
      301 cgcgcgagta tggcttcgaa tggtatgcgg gggtgccgtg ctcgcacctg acgccgttca
      361 tcaactacgt tctgcaggac cgctcgctgc attacctgtc catgaccaat gagggggatg
      421 ccgtggcgct gatagcgggc atcgccctcg gagcccgggc agaggggcat ggccgcgggg
      481 tcgccatgat gcagaattca ggactcggca atgccgtcag tccactgacc tcactgacct
      541 ggacattcca tctcccgcag ctgctgatcg tcacctggcg cggccagccc ggcagccacg
      601 acgagccgca gcatgcactc atgggtccga tcacgcctac cttgctcgaa acgatgcaga
      661 ttccgtggga gctttttccg agagagcggg acgagatcga tccggctctg cgccgggccg
      721 cttcccacat ggaggaaacg ggccggccct atgcgttgct catgcagaag ggctcggtgg
      781 ccgcatgcga gctgaagccc ggatcacagc agctgcaggc aaggggcgcc aaggcacagc
      841 cgatcgttcg gttgcgcggc cttgcgaccg agagccgagc ctcacgcaac gatgcgttgc
      901 gcgaagtgat cgcgcgcacg ccgagctccc gctgcgccgt gctggccacg acgggctatt
      961 gcgggcgtga gctgtatgcg atcgaggatc gccccaacca gctctacatg gtgggctcca
     1021 tgggctgcgt gttgcccttg gcgctcgggc tggccctggc gcggccggat ctgcgcgtcc
     1081 tggccgtgga cggcgatgga gcggcgctca tgcgcatggg agcgtttgcc accgccggcg
     1141 cctatgggcc gcctaacttc tcccatctgc tcctggacaa cggtgtgcac gactccaccg
     1201 gcggccaggc gacggtctca actcacgtct ccttcgccgg catagccgcc tcctgcggtt
     1261 acgcctcggc gctgcagacc gatgatctcg gggagatcgc cgagtggctg gacggtagtg
     1321 tgctggaggg atcacgcttc gcctgcctcc tcacacgcaa gggcacgcca gacagcttgc
     1381 cgcgaccgtc cgtcagcccg cgggaactga agttgcgatg gatgcagcat ttcgccccag
     1441 ctggagtcag gtgatgcagt tgctgctcaa ccccgggccg gtaacactct ccgagcgcgt
     1501 tcggcgcagc ttgttgcagc ctgatctgtg tcaccgcgag agcgagtttt tcgacctgca
     1561 ggacactgcc cgcgagctgc tcctcggcac ctatgagctc gcgccgaaga cctggacggc
     1621 cgtgctgatg accgcttccg gcaccggcgc cgtcgaaagt atggtcgcct ccctcgtacc
     1681 ggccaacggc cgcgtgctcg tgatcgagaa tggtgtatat ggggagcgca tcgcccagat
     1741 ctgtaagcag tacggcatcg cgcatgatcg cattcgcggt gactggatgc agccgccgga
     1801 cctggcggaa gtcgcgacac gactggattc cgctcgaggc gatgcagcct tcacccacat
     1861 cgccgtcgtt catcatgaga ccaccaccgg ccgcctgaac gatctgcctt cgctcgccga
     1921 tgtctgtcgc gctcgacgtg tgcgtttgct ggtggacgcc gtgagcagct ttggagccga
     1981 agccatcgat ttcgccgacg agaccgtggc ggccgtggcc gccacggcca acaagtgtct
     2041 gcacggtgtc cccggcgtcg ccttcgtgat tgcccggcgc gagtcactgg ccacggcggc
     2101 caaccggacc tattacctgg atctggcccg cctcgcacgc gcccaggacc agcgcaacac
     2161 gccgttcacg cccgccgtgc atgcttgcta tgcaatggtc gaggcgttgc gcgaatacgc
     2221 cgaggagggc gggcgtgccg cacgctttcg ccgctacgca gcgctggccg agcaggtgcg
     2281 ggccggactg gctgcgctcg ccatccgtgc cgtgctgccg ccggaggagt cctcggtggt
     2341 gctgcgcgcc tatacgttgc cggcgcacat gcagtatccg gccttgcacg atgctctgaa
     2401 ggctgggggc ttcgtgattt atgcgggtca gggagagctg gcaaaaatcc tgttccgcat
     2461 ctccaccatg ggaaatctga attcggccga catcgagcgg ctgctgacgt gcttcgcccg
     2521 tttgccagca aactgatcgg cggcgtcgcc ctcgccctgc tgctggggac gcgggcgctc
     2581 gcccagaccc aggtcccggc acccgatgcc agcggggccc agccctctgc gccgcccccg
     2641 gtcgagtcga gctggtacaa ccgtttgccg ttcatcccgg tgccggacat cggcagcgat
     2701 ccggacagcg ggaccacggt cggaatcctg cccgtctggc tcaaaaccga cgaccagcac
     2761 gccatccgcc ggatcattgc gccggatttc gtctacaacc ctttcttcgg ctacggcatc
     2821 gacgcgcgcc tgtatgccta cgactcggag gatgagcagt ggtcgatcga gggcggcgtc
     2881 aaggagcggg tcgagcgcga cttcgacgcc gagtggcagc gcggccggct ccgcgagggg
     2941 ccctggtcct acactgcgag cctcatctat agccgtgacg ggactccacg gttcttcggg
     3001 atcggcaaca actcaccagc cattgccgag acgaactaca ccaactcgca ggaactggcg
     3061 cagattcagg tcgggcacaa tttcagcccg gtctggcagc tgctgtacac gctgcgggtg
     3121 cgcacggtgc acgtgctgcc cggaacgctc gcccggattg cctccatcca gagtcgcttc
     3181 gcagatatcc tcggcatcgg gaccaacagc gaggtgctca atcggatctc gctcgtgtac
     3241 gacacgcgtg acaacctgac tgcgccccgc cggggcatgc aatgggtaat ctacggcggc
     3301 ctggcgactc gcaccgtcgt gcacgacaac accgacgcgg tctacgacga gacgggcatc
     3361 gatgggcgca gttatctgcc gatagctccg caaacgattc tcgccaccca cgtcgccctg
     3421 cgttacctgc ccagcgtgcg tcgcctgcct ttctgggcat tgagcagtat cggcgggggc
     3481 gagagtgtga tcggtggtga gcagccgctt cgaggctacg gtgccggccg cttttacgat
     3541 cgcaacgcat tttccgccag cgccgagctg cgccacaccg tgtggtcctt cgatgcgatg
     3601 tcgacgcacg tcgatatcga gttgaccccc ttcgttgatg tgggtcaggt gtttgcgcgc
     3661 accgggacct ggcccatcca ggatctgcac aaggtcggcg ggatcggctt tcgcggtgtc
     3721 gcccggcctt cggtggtcgg attcgtcgac gtcggttatg ggaacgaggg cgtggccgtg
     3781 ttcacgggga tcaactaccc gttctgatgt cagtgcgctg cttgcgcgcg accccgatcg
     3841 attgagccta tcgcagcagc gagcgcgcga tcagctcctt catgatctcg ttcgtgcccc
     3901 catagatccg ttgcacgcgc gcgtcggcga acatgcggca cacgaggtat tcgttcatgt
     3961 agccgtagcc gccatgcagc tgcacgcact catctagtac gtgctgctgc atgtcggtcg
     4021 tccaccactt ggccatggag gccgtcgccg tgtcgagcgt gccctcgacg acctttccaa
     4081 cgcagtcatc gacgaacgcg cgtgcgattc ttgcgaccgt ggcgatctcg gcgagtttga
     4141 agcgcgcgtt ctggaattcg gccaccggat ggccaaaggc tttgcgctcc ttcacgtagt
     4201 gcagggtcgc ctcgatggcg ccctccatgg cacctacggc cgccagcgcg acgatcatcc
     4261 gctcgtaagg aagatccgac atcatctgcg cgaacccccg gcccggctcg ccgcccagca
     4321 ggttggatgc ggggacgcgg acgtcctcga agaaaagctc ggcagtgtcc tgtgacttca
     4381 tgcccatctt gtcgagaatt cggccgaccc ggtacccggg tagatcgcgc gtctcgacca
     4441 tgaggatgga gatgcctttg gcacgctgag cgggatcggt cttcaccgcc agggcgagca
     4501 gccccgcgag gtagccgttg gtgatgaaga gcttcgagcc actcaccagg tattcatcgc
     4561 cctggcgctg cgcccgggtg cgaatggcct gaagatcgga cccggcgcca ggctcggtca
     4621 tggcgattgc gccgatcagc tcgccacggg ccatgcgcgg gagccagcgc tgcttctgct
     4681 cctcgaggcc atgattcaac aggtaatgcg cacagatgct gtgcaccccg gcgccgaagc
     4741 cactcatgcc ggtccgcgcc agctcctcgt gcacaacggc ctcgtgcaca aacgacccgc
     4801 ccactccccc gtattgcgcc ggcacatcgg cgcacagcag gcccagctcg ccggcacggc
     4861 gccagatctc cggcccgaca tgctgttgct tgcgccaggc agcatcgttc ggcgtcattt
     4921 cggccgcgat gaaccgggcg atcgcctcgc gaaagagtga cagctcttcg ttcattgcga
     4981 gtcaagggta acatggctgc cagtgcgcat tgaccggcgg agccggccga tgctgcagcg
     5041 ttccgcagcg ccccgcggga cccgatcgaa agagcttccg gccgatccgc gccgtcgcta
     5101 tactcacgcc gctttaggta cgcgcaaggt tcctcgtaga ggattcggtg agatcggcgg
     5161 gagtcactct ttcgccgacg ggtcgaaacg gccgcaggct gtgctcgacg atccgaagag
     5221 ggaacgcggt tggaatccgc gactgccccc gcaactgtaa ccggcgagcg accgtccaga
     5281 tggccactga ggttcgagcc tcgggaaggc ggacgcgagc gatgacccgg gagtcaggag
     5341 acctgcctcg cgccgtcgtc ctgttcccgg ccggggtgtg gcgggagctg cggggtatcc
     5401 gtcgtgacga cacggttgga gtcgtcgacg cggagccccg gttgaatgtg ccgcatgcgg
     5461 cagtcttcca ccccccgcgt aacggctcct ggattcaagc caggagaatg tatgcgcgaa
     5521 tcagtgttgc tttcgatgct tgcggcaacc gctttgtgga ccccgggctt ctcccgggcc
     5581 acggatgccg atgtggccgg cgcccccgcc gcggatgcgg agacacccga cagcgtcgtg
     5641 gtgacggcga ctttcattcc caccccggag caggaaattg ccagcagcat gacggtgatc
     5701 accgcccagg atatcgagac ccaccggcaa cagtccctgc cgcaggtgct gcgggatgtg
     5761 cccggcctca atgtcgtgca gccggggggt cctggcggat atacctcctt gttcatgcgc
     5821 ggcaccaact ccaaccatac gaaagtactg gtggacggta tcgatgtcac cgatccgagc
     5881 agcagcggct cgacgttcga tttcggccaa ttcctgagtc aggacatcga gcgggtcgag
     5941 gtcctgcgcg gcccgcagag cggcttgtac ggagcggacg ccatcggcgg ggtcatcaac
     6001 atcatcaccc gcagcggcgc gggcccggcg caggtgtggg gctcggcgga agccggctcg
     6061 ttcgatactt tcagtcaggc cgcaggcctc agcggtgccg cggaacagtt tcattactcc
     6121 gccaacctcg agcatctgca tgccggggcg actcccgtca cgccgctgga cctgctgttg
     6181 cctggcgaga agcgtcatga tgacagctat gacaatctca ctgcgtccac caagctgggc
     6241 ctcgatgtga cggagcactt cgatctgggg ctcgtcgcgc gctataccga ttcacatctg
     6301 cgcttcaccg gcgatgacga gttcgccttt ccgagcttcc cgcaagcgcg gcagagcgcg
     6361 agcaatacca gcgagtacta cgcccgtgcg ttcgctcacg gcgttgccgc agacggcttc
     6421 atcgatcaga ctctcggtgt cggcttcacg cgcaatcgct cggtgaactt cgacccggaa
     6481 atcccggcta cgctcgacat cggcgagcgc gccaaggtgg actggcgcgg cgccttgaag
     6541 ttctccgatg cccagaccct ggttctcggt gccgaacacg agcacgatga gatcagccag
     6601 ccggtctccg caggcgttgc aatcagcgcc gggtatggcg agttgcaatc ccagctcggg
     6661 gagcatctgt tctcggcgct caatctgcgt tacgacgcca acagccagtt cgggggcaaa
     6721 gcgacctacc ggctcgcgcc ggcatggctg atctccgaaa cgggcacgaa gctcaaggcc
     6781 agcatcggca gcggattcaa ggcgcccacc ctgggcgagc tgtatcagaa ttttccgcag
     6841 ttcttctttt tcggcaatcc gaacctcaaa cccgagacga gcatcggcta cgacctcgga
     6901 ttcgagcagt ccgtggcgca ggagacagtg cgcttcggag ccacgtactt tcgcaaccgg
     6961 atccgcaatc tcatcgacat tgcggcttcc ggcgcgacct acgcaaactt cggccgagcc
     7021 accactcaag gggtcgagag ctttttgtcc tggtcgccgg tgaagtcgct gctgttcaga
     7081 ctcgattaca cctacacgga agcgacggac gacatccggg atcaggagct gctgctcagg
     7141 ccgcgtcaca agggcagcct cgatgtgcgc tggcagccgc tgcagccgtt gtcactcgat
     7201 ctgaatgtgc tcgcagtggg cacctacatc gatggcaacc gggacttctc gattccgcgc
     7261 ctgcaggcgc ccggctatac gctcgccaat gccgcggtca acctggccat cacgccccac
     7321 ctgaccgcct atgcccgagt ggacaacatt ttcgatcggc actacgagag cccggttggc
     7381 tttctgcagc cctcgctcgg ggcgttcgtg gggctcagcg tgaagctgtg aggaactagg
     7441 ttcccgacac gcggggctgc cagccgcccc cgagtgcctt gatcagcagc acggcggccg
     7501 tgagccggcg cacctggatg tccgaggcgg tgagctcggc cgagagagcc gcgttttcgg
     7561 tgatgaccac ctgcagatag gtcaccacgc cgcccttgta ctggtattgc gtctgggtca
     7621 gcgctccggt ggttgccgcc accgccgcag cctgactggt gctttcgctc tccagatcgc
     7681 gaagggccgc gaggttgtcc tcgacctcct gataagcggt caacacggtg ttgcgatagt
     7741 ccgcgacctg ttcgtcgtat gccgcatgtg cagctgcaga ttgggccaga tagcgtccgc
     7801 catcgaaagc cgtcagcagc gcggaggggc caatagacca gaggcggctc ggggccgtga
     7861 tccagttgga agcctgagtg ctctcgaatc cgacgcgtcc gaacagatcg aagaccggga
     7921 aataggccgc ccgcgcgacc ccgatttgcg cattggcgga ggcgacgcgc cgctcggcgg
     7981 cggctacatc gggccgacgc tcgagcaggg ccgagggcaa cccgggatcc aggacgggcg
     8041 gcagcacctc gggcttgagc ggttgcgggt cgaggcgaaa gctcgaggga gactcgccga
     8101 tcagcacagc gatggcgtgc tcggtctgcg cccggcgcag gcgagcatcg gtggcctggg
     8161 ttcgcgccga ttcgagctgg gccttggatt gatcgacatc agcgagtgcc gcggcgccac
     8221 cgttgtacag atgctcggtg agctcgagcg cttgccgata gtcttcgacg gtccgatcga
     8281 gaagctcgag ctgcgtatcc tcaccgcgaa gagtgaaata gtccgcggcc agctcagcgt
     8341 gcgtgctcag atcgagtgtt gccaggtccg ccgcagtggc ctgctcggtg gcgcgcgccg
     8401 aggctaccga attgcgcacc cggccccaaa catcgatctc gtatgacaga tcggcatcga
     8461 gcacgaagtc attgccgatg gcgggcttgg tgttggtgta caggggcgaa ttgagggaca
     8521 cgcgcgaacg ggtagcggag ggcccgaccg tcaacgtcgg gaaataggct gcccgcgcca
     8581 cacgggtctg tgcgcgtgcc tcctgcaggc gtgcgaaggc cgccttgatg ttctgattgg
     8641 ccccggtcac gcgcgcctcg agcgtatcca gggtagggtc tccgaacagg ctccaccacg
     8701 gaccgcggct ctgcgcatcg gccggctgcg ccagcttcca gtccaccgac tccttccacc
     8761 gtggcggtgg cgtctcgacg ggcggtctgg agtatctcgg cgcgaacgag caggcagcca
     8821 ccgccgcgca aacaagcatt ggcagagtgc gatgcatgtg ctcagggtgc gcgggcggcc
     8881 ggtttcgggg cgctgccctg ttgcgctggc gggggccggg tccgcaccgt ggaaccctcg
     8941 ctgagggagt ccggcgggtt gacgatgatc tcgtcattgg ccgtcaggcc ggaaagcact
     9001 tccacggaag tgccgaagtc acggccctgg aggatgcctt tcatgtggac gtgattgccg
     9061 tcggtgaccg tcgcgacctg caggccctgt gcgcgaaaca ccagggcggt cgcggggaca
     9121 cgcaccgtgc tggagctgcc cggcagcttg aaatggactt cggcatacgc acccgggaac
     9181 aactcgccct cgctgttatc cacctggagt tccacctgca gcgtgcgcaa ggtgggatcc
     9241 agtgcctgag cggtgcggac cagggttgct ggatagtcct ccccgggatg ctcgttgaag
     9301 tgcagctgcg cttgcaggcc gaccgtcata cgtgcggcat acgactcagg cacgtccaca
     9361 tagatacgca attttttggt gtcggccacg cggaacagcc agctgccgga gctctgcccg
     9421 gcgttgatca aagcgccaat atcggtattg cggccggtga cgacgccatc gaagggcgcg
     9481 accaccctct tgaaggtctc gagctgctcg agtcgcgcct cgttcgcctc agccgaagcc
     9541 gccgaggcgc gtgtggccgc ggcatccgca gcgcgtgtgt ccgcatcctg ctgcgagacc
     9601 gcgtggttag ccagcaaacc ctgccacctc gtattcgtgc tctgcgccag ctgggcgttc
     9661 gcccgcgcgg tggcgaggtc ggctcgggac tgccgcagct gctgatcgac ttcgggcgtg
     9721 tcgatctccg cgaggagctg gcccttgctg accggagtac cgatgtcggt gtaccagacc
     9781 cgcaggtagc cgctggtgcg ggcgtagatc ggcgcttcga tgaaggcctg cacgttgccc
     9841 ggcagcacca gctcctcgga agcggggctg ccctccggat ggctgctgat gacgaccggt
     9901 accgcctcgt tggcggtctg tttgccgaga ctgctgcgcg aatgaacccg gctggcgaca
     9961 ccccagatcg ccagcagcac cgctaccacg agggcaatcc aggcgtaacg cttcaccctg
    10021 tgcactgtga tctcgtcggc agagggcggc acaggctcgg tcatgcgggg ctcccatcgg
    10081 ccgcttctcg ctgggctgtt cggccgtgga tcagcgtgaa caccgtggga acgaagaaca
    10141 gcgtggcgat cgttgcgaag acgagtccgc cgatgaccgc gcggccgagt ggcgcgttct
    10201 gctcgccgcc ttcaccgagg ctcagggcca tggggatcat gccgatgatc atcgcgagcg
    10261 ctgtcatcaa tacggggcga aagcgcgtga aacctgcttt cgaggcagcc gtgaatgcat
    10321 catcgccagc gttcatccgc tcacgcgcga agctgaccac gagaacgctg ttggcggtcg
    10381 ctacacccat gcacatgatt gccccggtga gagccggtac gctgacggtc gtgtgcgtca
    10441 ggaacagcat ccagacgatg cctgcgagcg ctgccgggag cgcggtgatg atgaccagcg
    10501 gatcgagcca ggactggaaa ttgaccacga tcaggaggta caccaggact accgccgcga
    10561 gcaggcccag caacagccca tggaaggagg cgttcatcgt ctgaacctgg cctcgcacga
    10621 cgacgtgcga tcctctaggg acctgtccct tggtggcttc cacgatcctg ttcacctgcg
    10681 tggagatgta gcccaggtcg gtatcctgca ccgatccata gatatcgagc acgggcgtgg
    10741 catcgtagtg actcacgacc gcaggactcg cgctgcggtg gaaagtggcg acattcgcca
    10801 gcagctgtcc ggcgccgccg cctccattgg tcaacggtgt cgtcgcgagc gagttcaggg
    10861 tttcgagctg cggctgcggc gtttgtgcga cgacgttgta ttgcgtaccg ctgtgcggat
    10921 cgatccagaa ggagggctgc gtctggaagc tgccagagag cgagaccagc atgttggagg
    10981 ccacgttctg ctgagtgagc ccgagctggc gcgccttggt ccgatcgacg tccacgttga
    11041 gcgtcggata atcgaaggcc tgctggatgt gcaggtcaac agcacccggc accgcacgca
    11101 acctctgcag aagcaggttg gcataggtcc ggttcgactg cagatcgttg cccacgacct
    11161 ggacgtcgat cgggctcggc agcccgaagt tgagcgtctg gctcacgata tcggccgggg
    11221 gaaaggagaa gctcgtggag gggaagccgg cattgagcac agagcgcagg gtgcgcatgt
    11281 aatccgccgt ggcgtgatgg ctcttcttca gagccacaaa gatgtccgca tcgctcggtc
    11341 cgaccggtgc ggacgtgctg taggagaggt tgattccgct gtaggggagg ccgatgttgt
    11401 cgacgatgct ttccagttcg cccgcaggaa tgacgcggcg gatgccggcc tcgactcgat
    11461 cgcacagcgc cgccgtctcc tcgatgcgca tcccggtgcg cgcacggaag tgaagcttga
    11521 tctgcccgga gtccacggcg gggaagaagt cctgacccaa gcccggcagg taggtcccga
    11581 atgggaaggc cagaatcgcc gacagcacca tcgcagccag gaagatgcct gcaaatcgca
    11641 gcccgccgtg aagcgccatt tccatcaggg cgtgatagcg gtcgcgagtg cgagtgaagc
    11701 cacgctcgaa tcccgcttga aagcgcgcca gccagccgcc tcggccggtg cgctcgtgct
    11761 cgtgcgtggt gagccagtac ttcgacaggg tggggaccag cgtccgtgac agcaggtacg
    11821 aggcgagcat cgcgaacacg acggcctcgg cgaggggcac gaacaggaac ttggcgattc
    11881 cggccagcat gaacatcgga acgaatacga tgcagatcgc gagcgtcgag accagcgcag
    11941 gcaaggcgat ctgctgcgca ccgtgcagga tcgagcgctc cacctcctca ccttcctcca
    12001 ggtgcgagtt gatgttctcg atcgtcacgg tagcgtcatc gacgaggatc cccaccgcga
    12061 gcgccagtcc gcccagcgtc atgatgttga gggtctctcc gagcgccgcc aggcaggcga
    12121 tcgaggcgag aatcgacaac gggatcgaga tgaaaatgat gatcgtgctg cgccagctgc
    12181 cgaggaacag caggatcatc agcccggtga gagccgccgc gatcgtcgcc tcgcgtacca
    12241 cccccgagat cgccgcgcgc acgaagatcg actgatcgcc gatcggctcg atgcgcagct
    12301 gcggcggcac catctcccgg atctgcggca gcttctgctt gatggcgttg atcacatcga
    12361 gcgtcgaggc cttgccgagc ttcagcacgg tcatgaggga ggcgcggcgg cctccgagac
    12421 gggcgatgtt ggtctgcggt ggcgagccat cccgaacgtg cgccacgtcg cgcacgtaga
    12481 ccacgttgcc gttcgcggtc gagctgatcg gcagatcgtt gagctcctcg atcttctgcg
    12541 ggctggcatt gagcccgatg taatactcga ggtcgccgat cttctgcgtg ccggccggca
    12601 ggatcaggtt ctgctgggtg atggccgccg tcacatccga cccggacagt ccgcgagcgc
    12661 gcagggccgg cagatcgagg tccacctgca cctggcgttg ccggccgcca tagggccagg
    12721 gaatggcggc gccggggatc gtggcgagcg cggtccggat gatgttgtta ccgagatcga
    12781 agatctgcgc ctccgagagc gactcactcg agagcgcgag ctggatgatc ggcacggtgg
    12841 aggcatcgta ggccaggatg aacggcgggg ttgcccccgg tgggaggttg cgcagcgaag
    12901 tctgcgagac gcccgtgatc tgggcgtagg agagcacttc atccgccccg ggctggaaga
    12961 agtacttcac caccgagatg ccgttcaggg actgcgattc ggtgtgctcg atgttgttca
    13021 cggtggtctg cgcgccgcgc tcggtcccga gcacgatgcg gttcgccatc tcgatgggct
    13081 gcaggccagc gtagttccag atcgccgcca cgaccggaat cttgatgctc ggaaagatgt
    13141 ccacggccgt gcgcaggatg gcgaacgtgc ccatcagaaa gatcagaatc gccagaacga
    13201 tgaaggtgta ggggcgctga agcgcgactt tgacgatcca catctggtgc ggccgctagt
    13261 gtgagtgcgc cgccatcaag gcgaggcggc ggtacgaggc gagcgatccg cgccagcacg
    13321 tgccataccc gagagcacgg cagagctcgt ggcacgcatg cggtacagtc caagctgggc
    13381 tcggagcaga aggtggcgcg ccctggcgcc tggcatcccc gagcatgaag ccgctgcctt
    13441 cagagtaccg ggccggttgc cgaggccggc ccgaccgcgc tggcggacat gcagtcctcg
    13501 tcctcgcact cggtgagctc gatctgcacg gtcgcgtgtt ggacgccaaa tcgctcccgc
    13561 aaggtagttt gaatatccac cagcacccga tcccgatcag cattctcgcg cagcaccgca
    13621 tgcaacgtga cgaccgggcg ctcatcggtg agggaccaga cgtgcaggtg atgaaccccg
    13681 gccaccgccg gaattgcagt ggacagcgcg tgctcgatct gctcgccatc gcaatccggc
    13741 ggagcgcctt cgaggaggat atgagccgat tcgcgactga tccgccagcc gctgcgtgcc
    13801 atgatgagtg ccacgacgcc ggagagcaga ggatcggcgg gcatccagcc ggtgagaacg
    13861 atgaccacgc cggcgatgag ggcggccagg cttcccagga tatcgcccat gacgtgagcc
    13921 agcgctccac gcacgttgag gttcgcctcg ccgcgcgaca ggaccacgaa tgcgagcaca
    13981 ttgaccaaca ggccggccac tgcaacgccg gctacgacct gtccctgcac gacggttccg
    14041 gtgcgcagcc gctgcacggc ttccgcggcg atccatacag caagcagcag cagtgccaga
    14101 ccgttcacga aggccgcgag cacttgccag cgatgatggc catagctcat cgaggccgtc
    14161 gccggacgag tacccgcgcg aactgcggcg tagctcatcg caagtgaggc acagtcggtg
    14221 agcatgtgcc ccgaatcggc cagcagggcg agagagccgg cgatccagcc cccggccgcc
    14281 tcgatcacca tgcagccacc ggtgaggagc agagcgccca tgagccgcga ctggctggtc
    14341 agagccccat gatgatgccc gtggccctcg tgcccgggcc gatgggagtg gcccggcccg
    14401 tggtgtccgt gcgagggatg aggctcaggt gcgccggcgc cgccctgcgc atgggagtgg
    14461 tcggcccccg cctcgtgatg gtgccccttg ggatggtgca cagtgtgtcg ctgcgaaaag
    14521 tgctcgacgc tgcaatggac gggggatcat agccgaaatc aaaaatcgta ggcgagtccg
    14581 gcgtggaatg tgcgatcgcg gcctggctcc agttgtcctc cgaagaggaa cacgcgcgag
    14641 tagtaatggc gatcacccag gttcgcgatt ccgcccagca gccgaaggtg gccgccaatg
    14701 acgtactctc cggagagatc caccacggca tacataggga tgcgcgcagg ggtgctgccg
    14761 acggccagat cactgtcctg gaaaaactgg gacgccacgc tgtcggcgat gagactgatc
    14821 ttgtagtgcc cttcctccct gagagtagcg cccgccttga gcacatagtc cggggcgtac
    14881 gccggtcgct tgccggtttg cccgggaatg atgctgctcg tgaagcgagc attcagcagg
    14941 ctcgcgttcg caaaaagggt cagatgctga ctcgactcag cctgcggcca caggcgcagc
    15001 acgtcgtagg ctgcctccag ctcggcaccg cggctgcgcg tatcgccggt attcacatcg
    15061 atcgtctcgg tctgggtgat ctgttgggac tcgatacgat ttctgacatt gacctgaaac
    15121 acgctcacgt cgtagtagag ccctgcgctc ggccagccgt gcacgcctgc ctcgtaggtg
    15181 aggtacctgg tgggatcagg attgttggtc ggcgagaaat tgctgaacgg gctggcgatg
    15241 tccaggtagc gcaggggacg aaagccctgc gacacgttga gataggtctc gttgccgcgt
    15301 ccgaagtcat tgccgatgcc gacaccaaag agcggcacgc tcttggagta cgtggcatcg
    15361 accagctgcg ggtgcggggc gacgctctcg tgtgtggaga gctcttcgtg gtccagccgg
    15421 gcggaggtca ccagatgaaa ccaggggaag cggaacacgt tctcggcgaa gatcgcgccg
    15481 tagtgggtct cgcgctcgtc gcgatagaac ggaaggccgc tctcatcggt gcgatccgca
    15541 agcggattcg ggtcgatgga ttccgtgtag ggacttcgcg accagtaggc cgtgtagccg
    15601 acggtgagcg cgttacccga tccccagcgg tgcagcagac gtccgtcgag ccccgtgtaa
    15661 tggaaacgct gaccggtcag catgccgccg gtcgccgagt catcgatccg gttgtaagta
    15721 tcggcgcgcg tgatgaggtc ggtgtagccg gtccagagct tctgcgtgag cagatcgtgc
    15781 tcggtcaaag cgttgtcata ggacaggaca gccgtgtagc ggtccgtcca gagccgatcg
    15841 tccggtgtcg tggcctggtc ggaattggcg cggaactggg cgccggacat gagccctgcg
    15901 agtccgctct cgagcgagta ggcgtgcacg gcaaaggtca gcgtctgctg ggtgtcgggg
    15961 cggaagccga cctgcagatc gcccgagttg agctcgtagt cgccgttccg gcgctgcccg
    16021 ctcgactgcc gatgcgaata gtccgccagg tagtcccagg tgccgagggt gccggagatt
    16081 gcattgaagc tcgagaacaa gccttcgtcg ccgccgatct gctcggtggt gcccgcgaag
    16141 gtctgggtcg ccggcgtgcg cgagacgaag ttgatgaccg gct
//
LOCUS       DASRHN010000226         9153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_743997, whole genome shotgun sequence.
ACCESSION   DASRHN010000226 DASRHN010000000
VERSION     DASRHN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743997"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFB37_08695"
     CDS             <1..265
                     /locus_tag="VFB37_08695"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZO22570.1"
                     /translation="PARHTYVAVSGLASVVRDPARARRLWSPEQRAFYPEGPEDARLA
                     LLKVKIEQAEYWIAPGPSAYLFAAAKAAVTGTPAGTLGENRKL"
     gene            364..1152
                     /locus_tag="VFB37_08700"
     CDS             364..1152
                     /locus_tag="VFB37_08700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO22571.1"
                     /translation="MKVALVTGAAQGLGLATASLLARLGFHLVLADIQALDAALARVR
                     EHDRAAEGAQGDISSEAFVRELSARIARDHRAAEVLVNNAGISLIAPAEETTAEQWQR
                     VMAVNLFAPFLLCKYLGTQMLGRGSGSIVNVASVAGLAGISHRSAYNTSKHGLIGLTR
                     TLAAEWGGRGVRVNAVCPAWIKTEMDVADQGAGAYSDQDIIDRVPLARFAKPEDVAQA
                     IAFLADGEKSGFVNGTCLTVDGGWTADGSWESLRTRTRTGPRRS"
     gene            complement(1149..3563)
                     /locus_tag="VFB37_08705"
     CDS             complement(1149..3563)
                     /locus_tag="VFB37_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO22572.1"
                     /translation="MKDSARAVIIGGGVVGASVLYHLAKIGWTDSLLLEKSELTAGST
                     WHAAGGMHTFNGEANISRLQKYTIDLYREIEAISGQSCSLHPNGGLMLAATQGELDSL
                     KLICSRARYLGMETEMISLEEALQLNPLVDPSHFIGALWRADGGHCDPSGTTQAYAKA
                     ARSLGAAVERFTRVLSLEQRRDGSWNVVTDKGTVRAEHVVNCAGLWAREVGQMVGIEL
                     PVLAMEHHYLLTEDLPELRGREREIVNTTDYSGEIYMRQERGGALIGTYEPQGIVWSP
                     LRTPDDFSMQLLPEDFERLAPYFEVGFRHFPALGRVGIRKAVNGPFTFAPDGNPLVGP
                     VRGIRNYWVACAVMAGFSQGGGIGLVLSRWMAENDPGQDILSMDVTRFGAFATPKYTS
                     IKVPENYSRRFRLAYPNEELPAARPVRRSPVYDRLKAAGAVMGANFGLEHALWFAPPG
                     VPASETPTYRRSEAFPIVRRECHNVRNAVGIYETSNYGKYEVTGRGARGWLDRVFASR
                     IPRPGRIGLGPMLNPAGRIVGDLSIACLREDRYLIMGSGFAEEFHLRWFEDTEPAADV
                     FVRSAASTLTGFSIAGPRSRELLQRLVRADLSAAQFRLFQVRETAVGFAPSILMRAGF
                     TGELGYEIWTTPDYQATLYEDLLEAGADLGLAHFGGRALSSLRLEKGYGSFNKDFRPD
                     YTAGESGLDRFIDLEKGEFTGRAAAVAERASGPKRRFVVMEVPQTETEVIGYESIMKD
                     GKPVGYVTSGAYGHCIDRSLAAGYVPSALAADGMRLEIDILGEMRTAIVRLQPLYDPQ
                     GLRLRS"
     gene            3627..4622
                     /locus_tag="VFB37_08710"
     CDS             3627..4622
                     /locus_tag="VFB37_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZO22573.1"
                     /translation="MAPEGNERHDGAFCLPSALTRKWTLRRLLARIFGALCATAVLAA
                     GAASPSPAPVIVSSKLSSESAMLGHMIRLLLDAHGIRTVDRMTLGATPVVRKALLSGE
                     VDLYVEYTGNAGFFFNIPSNPAWKDLWRGYELGSRLDLATNHIVWLTPAHASNAWALA
                     VRSDVALANKLQNMSDFARWVSSGGKVVLACSAEFANAGALRSLEKTYGFTLKSDQLI
                     VLAGGETSATIRAAAAHTNGTNTAMVYGTDGGISAANLKLLVDDRHDQPIYAPVPMIR
                     EASLRAHPQIAEIVKPLMETFDTQTLQRLNERVQIDGESAQAVAADYLKRRGFIR"
     gene            4619..5779
                     /locus_tag="VFB37_08715"
     CDS             4619..5779
                     /locus_tag="VFB37_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22574.1"
                     /translation="MSVRAYRIDRLGTLLSVLVAVALILLPFVVFKSNRIVPGEPKAL
                     AAVLPTWATIGFYACVLGVAAVAIGLSSARIRFVASLAGIVASAAALAAAADRLTPLG
                     NKVVRVAPGAGFWVLLLGLGLLTTDAVARMRLAPAARVLLLVLVLFVAYTTLADGIFD
                     NLSIMREYAVNADRFAVEARRHVGLVAGSLGLAVIVALPLGILCHRVPRLRPGVLGTL
                     NLIQTIPSIALFGILMVPLGELATSVPLAARLGISGIGAAPAATALFLYSLLPIVANT
                     VAGLRRVSPATVEAARGMGMTPLQVLSAIEIVLALPVILTGIRIVLVQNIGLTTIAAL
                     IGGGGFGTFVFQGIGQTATDLVLLGAIPTVALAFCAAVLLDALIEVLDRGKR"
     gene            5776..6711
                     /locus_tag="VFB37_08720"
     CDS             5776..6711
                     /locus_tag="VFB37_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22575.1"
                     /translation="MITLENLTKRYGEATVVDDVTLTIERNSITVIVGTSGSGKSTLL
                     RMINRLVEPTCGRVLIGGTDTTGEPAHLLRRRIGYAIQSHGLFPHRTVAENIAVVPRL
                     LGWEPARTQGRVKELLEIFQLDPATFADKFPHQLSGGQQQRVGVARALAAEPEVLLMD
                     EPFGALDPIIRTKAQEDLLNIQKRFNTTIVLVTHDIDEAFRLGNRVAVMSAGRVLQYG
                     RPAELLTQPADPLVARMTGVADRAMKLLSLTTAAEAARPGSSDGPAVAASASLREVLS
                     ELLWQGAASASVIEADGSCKGYLTVEAILSHGHPS"
     gene            6711..7451
                     /locus_tag="VFB37_08725"
     CDS             6711..7451
                     /locus_tag="VFB37_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22576.1"
                     /translation="MGSFGWIVRIAAFALLVSFLITPQSFAPLFVPLTQFGAPPIYDQ
                     GNLLDLALAHLATVCCAAAASAAVAVGLGILVTRPGGREFLPFSRTLVNIGQTFPPVA
                     VLALAVPLVGFGEKPTLIALFAYGLLPIFENTVVGLQTCPPAVLEAASGMGMSARQRL
                     RSVELPLAMPLILEGIRLSLVINVGTATIGSTVAAKGLGEVIIAGLLSNNTAFILQGG
                     LLTGLMAILLYDALAVLERRICANVRIE"
     gene            7530..8474
                     /locus_tag="VFB37_08730"
     CDS             7530..8474
                     /locus_tag="VFB37_08730"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZO22577.1"
                     /translation="MSITTADRTARTAQRPAIEMPTLLLLAFVYAGWLTTTFHYSRLS
                     LAISIPLTALLITLHSSLQHEMTHGHPTRWRSLNRLLAMVPLSLWMPYDRYLQTHHAH
                     HIDARLTDPVDDPESYYWAPDHWARLSPITRAIFRIEQTLAGRIIVGAFWRIWLFWCG
                     ELKALVHGVPSVRRMWLEHLIWLVPVVLWLKVVCGIPLWLYFVCMAVPANGIQLIRSF
                     AEHRAHPQVPARIAIVEDSWILGPLFLFNNLHSLHHESPGIPWYQCPARYRAARERLI
                     ARNGGLVYSTYFEVARRFLFRAHDVLPHPMGGIPRRAG"
     gene            complement(8514..>9153)
                     /locus_tag="VFB37_08735"
     CDS             complement(8514..>9153)
                     /locus_tag="VFB37_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZO22578.1"
                     /translation="LMCGYPIALGIAPVTPIAAPIPRAAWAAGRAVYRTDLIVRDDSP
                     WRNLEDSFGSRAGWTVTHSQSGFNAFRHELLKHRTAGRPALYRQMLGKLVTARNVLDS
                     VRENRIDIGPMDAYWHMLIARHAPHLTAGVRVLASTPLTPMPAFVAARRLPVECVARL
                     RTAFLNAASEDWFERYADLLLLEGFAAVETASYAPLLEWDREARTAGFELPA"
BASE COUNT         1497 a         3000 c         2954 g         1702 t
ORIGIN      
        1 tccggcccgg cacacctatg ttgcggtgag cggtttggcg agcgtggtcc gcgatcccgc
       61 tcgcgctcga cggctgtggt cgcccgagca gcgcgcgttc tacccggagg gtccggaaga
      121 tgcacgcctc gcactcctca aggttaaaat cgaacaggcc gaatactgga tcgcaccggg
      181 cccctccgcg tatctgtttg ccgcggcgaa ggctgccgtc accggaacgc ccgccggaac
      241 acttggagag aaccgcaagc tctgaatcag acagcccacc cgatgcggtg ggcacttgcg
      301 gacccggggc tcagaagcgg ctcgggacgg cgcgtcgcgt gctcgcgcgg aaggagagca
      361 aacttgaagg tcgctctggt aaccggggcc gcgcaaggtc tgggtctcgc caccgcgagc
      421 cttctcgccc ggctcggctt tcacctcgtt ctcgccgata ttcaggctct cgacgcggcc
      481 cttgcgcgcg tacgcgagca cgatcgagcc gccgaaggcg cccagggcga catctcctca
      541 gaggccttcg tgcgcgagct gagcgcgcgc atcgctcgtg accaccgcgc cgccgaagtg
      601 ctggtcaaca atgccggaat cagcctgatc gctccagccg aggagacgac ggccgagcag
      661 tggcagcgcg tgatggcagt caacctgttc gcgccctttc tgctttgcaa gtatctcggc
      721 acgcagatgc tcggccgcgg aagcggcagc atcgtgaatg tcgcctcggt cgccgggctg
      781 gccggcatca gtcaccgaag cgcttacaac acttccaaac acggcctcat cggcctgacg
      841 cgcaccctgg cagccgaatg gggcgggcgc ggagtgcggg tcaacgcggt ctgtccagcg
      901 tggatcaaga ctgaaatgga tgtcgcggat cagggcgccg gcgcctactc cgatcaggac
      961 atcatcgatc gggtgcccct ggcacggttc gcaaagcccg aggacgtcgc gcaggcgatc
     1021 gcatttctgg ccgacgggga gaagagcggc ttcgtgaatg gaacctgcct gacggtcgat
     1081 ggcggctgga cggctgacgg cagctgggaa agcctgcgca ctcggactcg cacgggccct
     1141 cgccgcagct aactcctcag ccgcagcccc tgcggatcgt agagcggctg cagccgcacg
     1201 atcgcggtgc gcatctcgcc aaggatgtct atttcgagcc gcattccgtc tgccgcgagc
     1261 gcggagggca cgtatccggc ggccagactc ctgtcgatgc agtggccata ggcaccggag
     1321 gtgacatagc cgacgggttt gccgtccttc atgatcgact cgtagccgat gacctcggtc
     1381 tcggtttgcg gaacctccat gaccacgaag cgccgcttgg ggccgctcgc acgctccgcc
     1441 acggcggctg cccggccggt gaattcgcct ttctcgagat cgatgaatcg gtcgagcccg
     1501 ctctcgccgg cggtgtagtc gggccgaaag tccttgttga acgagccgta gcctttctcc
     1561 aggcgcaaag aggacaatgc ccgaccgccg aaatgcgcca gccccaggtc cgcgcccgcc
     1621 tccagcagat cctcatacag cgtggcctga tagtcgggtg tcgtccagat ctcgtagccg
     1681 agctccccgg tgaagccggc ccgcatgagg atcgagggtg caaagccgac cgccgtctcc
     1741 ctgacctgaa ataacctgaa ctgcgcggcg gacagatccg cgcgcacgag ccgctggagc
     1801 agctcgcggg agcgtgggcc cgcgatcgag aagccggtca gcgtggaggc cgccgaccgc
     1861 acgaatacat ctgcagcggg ctcggtatcc tcgaaccagc gcaggtggaa ttcctctgcg
     1921 aagcccgatc ccatgatcag atagcgatcc tctcgcaggc acgcgatcga cagatccccc
     1981 acgatccgtc ccgccggatt caacatcggc ccgagcccga tgcggcccgg gcgagggatg
     2041 cgcgaggcga agactcgatc cagccagccg cgagcgcccc ggccggtgac ttcgtacttc
     2101 ccgtagttgc tggtctcgta gatcccgacc gcattgcgca cgttgtgaca ttcacgccgc
     2161 acgatgggga aggcttccga gcgcctgtag gtaggggtct cgctggctgg cactcccggc
     2221 ggagcgaacc atagcgcgtg ctccagcccg aagttcgcgc ccatcacggc accggccgcc
     2281 ttgagccggt cgtacacggg cgagcgcctg acgggtcggg ccgccggcag ctcctcattc
     2341 ggataggcaa gccggaaccg gcgcgagtag ttctcgggta ccttgatcga ggtatatttc
     2401 ggcgtggcga aagccccgaa gcgtgtcaca tccatcgaca gaatgtcctg gcctggatcg
     2461 ttctcggcca tccagcgcga aagcacgagt ccgatcccgc ctccttggct gaaccccgcc
     2521 atcaccgcgc aggctaccca gtagttgcga atgccacgca cgggtcccac cagcggattg
     2581 ccatcgggag cgaacgtgaa gggaccattg acggctttgc ggatgccgac gcgcccgagg
     2641 gcggggaaat ggcggaaacc aacctcgaag tagggcgcga gcctctcgaa gtcctcgggc
     2701 aggagctgca tggagaaatc gtccggggtt ctgagcggag accagacgat gccctgcggc
     2761 tcataggtgc cgatgagagc gccgccgcgc tcctggcgca tgtagatctc gcccgaataa
     2821 tcggtcgtgt tgacgatctc ccgctcccgg ccgcgcagct cgggcaggtc ttccgtgagc
     2881 agatagtggt gctccatcgc caggaccggc agctcgatgc ccaccatttg gccgacttca
     2941 cgagcccaga gcccggcgca gttcacgaca tgctcggcgc gcaccgtgcc tttgtcggtg
     3001 acgacgttcc acgagccatc gcgacgctgc tcgagagaca acacgcgggt gaagcgctcg
     3061 acagcggctc ccaggctgcg ggctgccttt gcatacgcct gggtcgtgcc cgaggggtcg
     3121 caatggccgc catccgcacg ccacaaagca ccgatgaaat gcgacggatc gaccagggga
     3181 ttgagctgca gtgcctcctc caggctgatc atctcggtct ccatgccaag atagcgggcg
     3241 cgggagcaga tgagcttcag actgtcgagc tcaccctgcg tcgctgccag catcaggccg
     3301 ccgttcggat gcaggctgca agactgaccc gagatcgcct cgatctcgcg gtacagatcg
     3361 atggtgtact tctgcaggcg cgagatgttg gcttcgccgt tgaacgtgtg catgccaccg
     3421 gccgcatgcc aggtcgatcc cgccgtcagc tcgctcttct ccagcagcag gctatccgtc
     3481 cagccgatct tcgcgaggtg gtagagcacg ctggccccga cgacgccgcc cccgatgatc
     3541 acggcccgcg cgctatcctt catgtcaggt tttctccggc tgggttcgat tggcgtggcc
     3601 cgcatcctcc gctgccatca taatgcgtgg cgccggaagg caacgagcgg catgatggcg
     3661 ctttctgcct gccctcggcg ctgacgagga agtggacact gaggcggtta ctggcgcgga
     3721 tcttcggagc cctttgcgca actgcagttc tggcggcagg ggcagcctcg ccatcgcccg
     3781 cgcccgtcat cgtctcctcg aagctctcga gcgagtcggc catgctggga catatgatcc
     3841 ggctgctgct ggatgctcat ggcatccgga cggtcgaccg catgacgctc ggggcgacgc
     3901 ccgtggtgcg caaggcgctg ctttcgggcg aagtcgatct gtacgtcgag tacacgggca
     3961 acgccgggtt cttcttcaat ataccctcga atccagcatg gaaggacctg tggcggggct
     4021 atgagctggg ctcgcgactg gatcttgcca cgaatcatat cgtgtggctg acgcccgccc
     4081 atgccagcaa cgcctgggcg ctcgcggtgc gcagcgatgt ggcgcttgcg aacaaactgc
     4141 agaacatgag cgactttgcg cgctgggtct cctcgggtgg gaaagtcgtg ctggcctgct
     4201 cggcagaatt tgcgaacgcc ggggcgctgc gctcgctgga gaagacctat ggattcacgc
     4261 tcaagtccga tcagctcatc gtgctcgccg gaggggaaac ttcagccacg atccgggcgg
     4321 cagccgcgca caccaatggc accaacacag cgatggtgta cgggaccgat ggcggcatct
     4381 cggcggcgaa tctgaagctg ctggtcgatg accggcacga tcagccgatc tatgcgccgg
     4441 tacccatgat tcgcgaggca agcctcaggg cccacccgca gatcgccgag atcgtgaagc
     4501 cgctgatgga gactttcgac acccagaccc tgcagcgctt gaacgaacgg gtgcagatcg
     4561 atggggagtc cgcgcaggcg gtggcggctg actatctgaa gcgccggggc ttcatccggt
     4621 gagcgttcgg gcctaccgga tcgatcggct cggcacgctg ctcagcgtgc tcgtcgccgt
     4681 ggcactcatc ctgttgccct ttgtcgtctt caagtccaac cgcatcgtgc cgggtgagcc
     4741 gaaagcgctc gcggctgtcc tgccgacgtg ggccaccatc ggcttttatg cctgtgtgtt
     4801 gggggtcgcg gcagtggcga tcggcctctc gagtgcgcgc atccgctttg tggcgtccct
     4861 ggccggcatc gtggcctccg cggcagctct cgcggctgcc gccgaccgcc tcacaccact
     4921 tggcaacaaa gtcgtgcgcg tcgctccggg agcgggcttc tgggttctgc tgctcgggct
     4981 cggtctgctg accaccgatg ctgtcgcccg gatgcggctg gccccagcag cccgcgttct
     5041 gctgctcgtg ttggtccttt tcgtcgccta cacgaccctg gcggacggca tcttcgacaa
     5101 tctttccata atgcgcgagt acgccgtgaa cgccgatcgt ttcgcggtcg aggcaaggcg
     5161 tcatgtaggg ctcgtggcag gatcgctcgg tctggccgtt atcgtggcgc tgccgctcgg
     5221 catcctctgc caccgcgtgc ctcgtctacg accgggtgtt ctggggacac tcaacctcat
     5281 ccagacgatc ccgtccatcg cactcttcgg catcctcatg gtgcccctgg gagagctggc
     5341 cacctcggta ccgttggccg cccggctcgg gatctcgggc atcggcgcgg caccggctgc
     5401 aaccgccttg ttcctttatt ctctgctgcc tatcgtggcc aacacggtag ccgggctgcg
     5461 gcgcgtctcc ccggcaactg tcgaggcggc ccgagggatg gggatgaccc ctctgcaggt
     5521 gctctcggcc atcgagatcg tgctggcttt gccggttatc ctgacaggca tccgcatcgt
     5581 gctggtgcag aacatcggcc tcaccaccat tgccgccctg atcgggggag gcggattcgg
     5641 gacgttcgtc ttccagggaa tcggccagac cgcgactgac ctggtgctgc tgggagcaat
     5701 tccgaccgtg gcgctcgcct tctgtgctgc ggtgctgctc gatgccctga tagaagtgct
     5761 ggatcggggt aaacgatgat cacgctggaa aacctgacca agcgctatgg agaggccacg
     5821 gtcgttgacg atgtcacgct caccatcgag cgtaattcga tcacggtcat cgtcggcacg
     5881 tccgggtccg ggaagtcgac cctgctgcgc atgatcaatc ggctggtcga gccgacttgc
     5941 ggccgagtcc tgatcggagg gaccgacacc accggcgagc cggcgcacct gctgcggcgg
     6001 cgaatcggtt atgcgatcca gagtcacgga ctgtttcccc atcgcacggt ggccgagaac
     6061 atcgcggtcg tgccgcggct gctgggatgg gagcccgcgc gcacgcaggg gcgtgtcaag
     6121 gaactcctgg agatcttcca gctcgatccc gccacatttg ccgataaatt cccgcaccag
     6181 ctctcgggag gtcagcagca gcgagtgggg gtggcgcggg cgctggcggc cgagccggag
     6241 gtgctgctga tggatgagcc tttcggagcg ctcgatccga tcatccgcac gaaggcgcag
     6301 gaggatctgc tcaacattca gaagcgcttc aataccacga tcgtgctggt cactcatgac
     6361 attgacgagg ctttccgcct cggtaatcgc gtggccgtca tgagcgcagg acgtgtgttg
     6421 caatatgggc gcccggcgga gctcctcacc cagccagccg atccactggt cgcgcgcatg
     6481 accggagtcg ccgaccgggc gatgaaactg ctctcactga cgacggcggc agaagctgcc
     6541 agacccggca gctcggacgg ccctgcggtt gccgcttcgg cgtcattgcg ggaggtgctc
     6601 tcggagttgc tctggcaagg cgcggcatcg gcctcggtca tcgaggccga tggcagctgc
     6661 aagggatacc tcactgtgga ggccattctc tctcacggac atccgagctg atgggcagct
     6721 tcggctggat cgtgcggatt gccgcctttg cgctcctcgt cagctttctg atcacgccgc
     6781 aaagcttcgc gccgctgttc gtgcctctga cgcagttcgg cgccccgccg atctatgacc
     6841 aggggaatct gctggacctg gcgctggccc acctcgcgac cgtttgctgc gctgcagcag
     6901 caagcgcggc ggtcgccgtc gggctgggca ttctcgtgac ccggccgggg ggccgggagt
     6961 ttttgccgtt ttcccgtaca ctcgtgaaca tcggccagac ctttcccccg gtggcggtgc
     7021 tcgccctggc cgtgccgctg gtgggttttg gtgaaaaacc caccctgatc gcgctttttg
     7081 cctacggcct gctgccgatt ttcgagaaca cggtcgtcgg tctgcagacc tgcccgccgg
     7141 cggtgctcga ggcggctagc ggcatgggca tgagtgcgcg tcagcggctt cgatcggtgg
     7201 agctgccgct ggcgatgccc ctgattctgg aggggattcg cctctcgctg gtgatcaacg
     7261 tcggtaccgc caccatcggc tcgacggtgg ctgccaaggg gctgggtgag gtgatcatcg
     7321 ccggtcttct gtcaaacaat accgccttca tcctgcaggg cggcttgctg acgggcctga
     7381 tggcgatact tctgtatgac gcgctggccg tcctggagcg tcggatctgc gcaaatgtcc
     7441 gcatcgaatg agaactagac cccatggagt ttgattgaac ttaggttaga atcattgcaa
     7501 catgtttgtg ggggtcgctc agcactagaa tgtccattac gacagccgac cgcactgccc
     7561 gtactgcgca gcgccccgcc attgagatgc cgacgctgct gcttctcgct ttcgtctacg
     7621 ccggctggct tacgaccacc ttccattatt cccgactgtc gctcgcgatc tcgatcccgc
     7681 tcactgcgct gctgatcacc ctgcacagct ccctgcagca tgagatgact cacggtcatc
     7741 ccacacgctg gcgcagcttg aatcgtttgc tggcgatggt gccgctgtca ctgtggatgc
     7801 cgtacgaccg ctacctgcag acacaccacg cgcatcacat cgacgcacgc ctgaccgacc
     7861 ccgttgacga ccccgagtcc tactactggg cgccggatca ctgggccagg ctgagtccca
     7921 tcacccgcgc gatcttccgg atcgagcaga ccctggccgg acgtatcatc gtgggcgcgt
     7981 tctggcggat ctggctgttc tggtgcggcg agctcaaagc actggtgcac ggcgtgcctt
     8041 cggtacgccg catgtggctc gagcacctga tctggctggt gcccgtggtg ctgtggctga
     8101 aagtcgtctg cggcatcccg ctgtggctct actttgtctg catggcggtc ccggcgaacg
     8161 gcatccagct catccgctcg ttcgccgagc atcgggccca tccgcaggtg ccggcgcgga
     8221 ttgcgatcgt ggaggactcc tggatcctgg gtccgctgtt cctattcaac aatctgcatt
     8281 cgctccacca tgagtcgccc ggcattccgt ggtatcagtg cccggcgcgc tatcgggccg
     8341 cccgggagcg gctgatcgcc cgtaacggcg ggctggtgta ctcgacttac ttcgaggtcg
     8401 cgcgccgctt tcttttccgc gcacacgatg tgctgcccca tcccatgggc ggcatcccgc
     8461 gtcgcgccgg ctgatcctga acctgttggg gtaatccccg gcgcggcgtc ccctcaggcg
     8521 ggcagctcga agcctgccgt tctggcctct cgatcccact ccagcaacgg tgcatagctc
     8581 gccgtctcga cggccgcgaa accttccagc aggagaaggt ccgcgtagcg ctcgaaccag
     8641 tcctccgagg cagcgttgag gaatgccgtt cgcagccgcg cgacgcactc tactggcagg
     8701 cgcctcgcgg ccacgaaggc aggcatgggg gtcaggggtg tactggcaag tactcttacg
     8761 cccgcagtca gatgcggcgc atggcgggca atcagcatgt gccagtaggc gtccatcggc
     8821 ccgatgtcga tccgattttc tcgaacgctg tcgagtacgt tgcgtgctgt caccagcttg
     8881 ccgagcatct gtcgatagag cgctggacgc ccggccgtgc ggtgcttcag cagctcgtgg
     8941 cggaaggcgt tgaatccgga ctgcgagtgc gtcaccgtcc agccggcgcg cgagccgaag
     9001 ctgtcctcga gattccgcca gggcgagtcg tcgcgaacga tgagatcggt gcggtatacc
     9061 gcccgaccgg cggcccatgc tgcccgcgga atgggtgccg cgatcggcgt gacgggcgcg
     9121 atccccagcg cgatcgggta gccgcacatg agc
//
LOCUS       DASRHN010000227         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1231618, whole genome shotgun sequence.
ACCESSION   DASRHN010000227 DASRHN010000000
VERSION     DASRHN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231618"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..473
                     /locus_tag="VFB37_08740"
     CDS             279..473
                     /locus_tag="VFB37_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZO22579.1"
                     /translation="MNDNQSSGIGKQVKGAIKEGTSKITGNKSGEVEGKIEKNIGKAQ
                     TKLGDRQADSRQDSEAKDRR"
     gene            847..2775
                     /locus_tag="VFB37_08745"
     CDS             847..2775
                     /locus_tag="VFB37_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZO22580.1"
                     /translation="MALVKKSKIARGLGNVPGPAAAQKPAGPIAKVNARASASDPRHQ
                     TVSERIAAATEELASGLSQAAAATKQLGRSMEQIAGGAEEAAGASQEQSAAVKRIAAS
                     LIGARSEADASSRRTDSVAVSLAEASARILTSVQAIERGAQRQTESISLITELDRRAK
                     DIGEITRAVSRIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLAETSDRSAREV
                     QQLTEAMQNEVNEVGGALKAAAAKALGDANAAAKVAQGLEARRQEMTQIAAGSRSTLT
                     ATLEAERAANEAQKGAEQIASAAEEQSAGAGEAQTAIEQQAKSLEQAQTAAHGLAILA
                     EQLRAGKAGAAAVEQISASAEELSASIQELSSAATQVMAAVEQINKAAQLQSSATQQT
                     SAALAQIENSAKVAQKSGRTSDDRVRLLDEALKEGRKAVENLVMSVSDALQDTRSSAA
                     TVKRLENVGRKVEKILEAIALVAVQTSMLAVSGSVEAARAGESGRGFAVVSNDIRSLS
                     REASDNVERAKDTVRGILEQIAALRGDLEQIIVATEMEVQNNRTVSGALQTIEGDFAA
                     LGVASRSILEGADNILGATGEMAKAARQVATAAEQASTASREAAAAATQQSRGAEDLA
                     AAIEEIAALADELKQRSS"
     gene            2790..4301
                     /locus_tag="VFB37_08750"
     CDS             2790..4301
                     /locus_tag="VFB37_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZO22581.1"
                     /translation="MARESSDRFLTFRVDTQLYALRADEVTEVIRPPTLARVPQGPAA
                     LLGLANLRGSVLPIASLREMLGKPAAAVTETARAIVLDIGAPIGLMVDCVEALESAAS
                     QQVETLQNDLSAAPAEQLLGAFSTHADRRVAKILDIRSMLKVAFANRSRAQSHTHKRL
                     AAAPALAARARNAAKTETLVTFEIAGQEFALPLEAVEEILPAPASVTAVARAEAVVLG
                     VTSVRDTLLPLLSLRALLGFSAATDSNDRQKIVVMRIGGAAVGLVADRARAILAADLE
                     MVDPIPPVLAARTGGESRVRAVYRAEAGRRLISILSPDQLFREDVMKRLAGARQASST
                     PALQADSGAREEMSFVVFRLGSEEFGLPIDTVVEVAQVPQQITRVPKTPKFLEGVVNL
                     RGEVLPVVDQRRRFEMPKLDNAEGRRLVVIRTERHRAGLIVDSVSEVLRTTADSVEPP
                     PELTDQVSRLVRGVINLPKSNRMVLLLDAAELLTRSEQGLLDSFQAKSKKANE"
     gene            4298..>5201
                     /gene="cheB"
                     /locus_tag="VFB37_08755"
     CDS             4298..>5201
                     /gene="cheB"
                     /locus_tag="VFB37_08755"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HZO22582.1"
                     /translation="MIRVLVVDDSALVRKLFGRVLSGEPDFAVTFARDGLEALAQLEA
                     FRPDVITLDVQMPQMDGLECLDRIMVQRPSPVVMVSTMTAEGAEATLDALRLGAVDFV
                     TKPAGAVSLRIDEFAPTLKQKIRAAAGARLPASRRLKERVQFRAGATPTRKPRKLEVP
                     EEHIAAIQGAGIVLVGTSTGGPPALEALLVQLPASFPWPILVAQHMPASFTGPLAKRL
                     DGICPLQVIEAAQPMPLQAGCVYIGRGDADLLVSKRPSGLIVRAVPAKADYPWHPSTD
                     RLVRSAMEHVPASQLIGVLMTGMGN"
BASE COUNT         1013 a         1589 c         1740 g          859 t
ORIGIN      
        1 cccgccgtgt ccaactcggc aagcaaagtg cgtacatcgg gcggggacat agctgtgtgg
       61 cgaccggatg agttctgcga ctctcaaacc agcataccgg ccaacactca gcgagcctat
      121 acgtacacaa cgcatggcgg ccctgccgat caggctcccc ggaagtgccg ccctcccatc
      181 cggcgccagg cccctgaggc gttcagggag tcttgcgcgc gaggaaactt tcgggggtct
      241 gaggcgtttc accaacccct accgaaggag tattagacat gaatgacaat cagagcagcg
      301 ggatcggcaa acaagtcaaa ggcgccatca aggaagggac gagcaagatc acgggtaaca
      361 agtcaggcga agtggaaggc aagatcgaga agaacatcgg caaagcgcaa accaaactgg
      421 gtgaccgaca agccgactcg cgccaggact cggaagccaa agatcgccgc tgagggatgc
      481 tgaccctaga gcagctgcgc gtgcatcgat tcaacgatgc gcgtcgtggc gctgcgacgc
      541 tgatcagcgc agccggactt gctgcgaact cacacggcgg cgccatcgag cgcggcccat
      601 gagcagacgg atgctctggc gagagcacat cttccgcgcg tggccgggcg gcgcgccgac
      661 cgttgtcttg cgccgccacg cgcggtccgt aaagtcttag ccaaaccctg cagaattacc
      721 taatattgag gcgcttctct atccagggtt tcaggcactc aagcgtgccg cggtccccgg
      781 cgcgcacaag aacgctatgc cttcgctcgc cccggggccc gacctgccgt cctccatgag
      841 ggtcacatgg ctttagtgaa gaaatcaaag atcgctcgtg gcctcggcaa tgtgcctggg
      901 ccggccgcgg cgcaaaaacc ggcaggtccg attgccaagg tgaatgctcg agcttccgca
      961 tccgatcccc gtcatcaaac tgtatctgaa cgcattgccg cggccactga agagctggcg
     1021 agcgggctgt cgcaggcagc ggcggcgacc aaacagctcg gtcgatcgat ggagcagatt
     1081 gccgggggcg ccgaagaggc tgcgggggcc tcgcaggagc agtccgccgc cgtcaagaga
     1141 atcgcggcca gtctgatcgg cgcgcgcagc gaggcggatg catccagccg tcgtacggat
     1201 tcggtagcgg tgtcactggc ggaggcctct gctcgaatcc tgacctcggt gcaggcgatt
     1261 gagcgcggcg ctcagcgtca gaccgagtcg atctccctga ttaccgagct cgaccgtcga
     1321 gcgaaggata ttggggagat cacgcgcgca gtgagccgga tagccgatca gaccaatctc
     1381 ctcgcgttga atgcggctat cgaagcggcg cgcgcaggtg aacagggccg cggattcgcg
     1441 gtcgtcgcag atgaggttcg caccctcgcg gaaacatcag accggagcgc acgtgaagtt
     1501 cagcagctta ccgaggcaat gcagaacgaa gtcaacgagg tgggcggagc gttgaaagcg
     1561 gccgccgcaa aggctctcgg cgatgccaat gccgcggcca aggttgcgca gggtctggag
     1621 gccaggcgac aggagatgac acaaattgcg gccggcagtc gcagcaccct gactgctaca
     1681 ctggaggctg agcgtgcggc aaacgaggct caaaaaggcg ccgagcagat agcgagcgcg
     1741 gccgaagagc agtcggccgg tgcaggtgag gcacagacgg caatcgagca gcaggccaag
     1801 tcgctggagc aggctcagac cgctgcgcac ggtttggcga tcctggctga gcaactacgc
     1861 gcgggaaagg ccggcgcggc cgccgtagag cagatcagcg ccagcgccga agagctgtcg
     1921 gcgtccatcc aggagctctc gagcgcggcc acgcaagtca tggccgcggt cgagcagatc
     1981 aataaagccg cgcaattgca gtcctccgcg acccaacaga cgtcggcggc tctggcccag
     2041 atcgagaaca gcgccaaggt cgcgcagaaa agcggcagga cttccgatga tcgcgttcga
     2101 ctgctggatg aggcgctgaa ggagggccga aaggccgtcg agaatctcgt gatgagcgtg
     2161 tcggacgctt tgcaggacac gcgctcgagt gcggccaccg tcaagcgact ggagaatgtg
     2221 ggtcgcaagg tcgagaagat tctcgaggcc attgcgctgg ttgccgtgca gacgagcatg
     2281 ctcgcagtga gcggatcggt cgaggcagcg cgagccggtg aatccggcag aggctttgcc
     2341 gtcgtttcca acgatattcg cagtctgtcg cgggaggcct ccgacaacgt cgagcgtgcc
     2401 aaggatacgg tgcgtggaat tctggagcag attgccgctc tcagaggcga tctcgagcag
     2461 atcatcgtgg caacggaaat ggaggtgcag aacaatcgca cggtctccgg cgcgctgcag
     2521 acaattgaag gcgacttcgc cgcccttggg gtggccagcc gttccatcct cgaaggcgcc
     2581 gacaatatcc tcggcgccac gggcgagatg gcgaaggctg ccagacaggt tgccacggct
     2641 gccgagcaag ccagtacagc ctcccgcgag gcggccgctg cggcaaccca gcaatcgcgc
     2701 ggcgccgagg atctcgccgc cgccatcgaa gagatcgccg cgctggctga tgagctgaag
     2761 cagcgttcgt cctgagcgga cagcagtcca tggctcggga atcctctgac cgcttcctca
     2821 ctttccgcgt cgatacacaa ctgtatgcgc tgcgcgcgga tgaggtgaca gaggtcattc
     2881 ggcccccgac cttggctcgt gtgcctcagg ggccggcggc gctgcttgga cttgccaacc
     2941 tgcgtggctc ggtgttgccc atcgcaagtc tgcgggaaat gctgggcaag ccggcggccg
     3001 cggtgacgga aactgcgcgt gccatcgtac tggatatcgg agcaccgatc ggtttgatgg
     3061 tggattgcgt cgaggccctg gagagcgcgg ccagccagca agtcgagacg cttcagaacg
     3121 acttgagtgc ggcgcccgcc gagcagcttc tgggtgcgtt ctcaactcac gctgaccggc
     3181 gcgtggcgaa gattctcgat atccggtcca tgctgaaggt ggcattcgcg aatcgctcgc
     3241 gggcgcaaag ccacacccac aagaggctcg ctgccgcgcc tgccctggca gccagagccc
     3301 gtaacgcggc aaagaccgaa accctggtca ctttcgagat tgcgggacag gaattcgcgc
     3361 tgcctctgga ggccgtggag gaaatcttgc cagcgcctgc ttcggtcacg gcggtggccc
     3421 gcgccgaagc cgtggtgctc ggggtcacct cggtgcggga taccctgctg cccctgctct
     3481 ctttgcgggc gctcctggga tttagcgccg caacggactc gaatgatcgg cagaagatcg
     3541 tggtgatgag aatcggcggt gctgcggtcg ggctggtggc cgaccgcgcc cgcgccattc
     3601 tcgcagcgga tctcgagatg gtggacccca ttccaccggt gcttgccgcc cgcaccggcg
     3661 gtgagtcacg cgtgcgagcg gtctaccggg cggaagcggg aaggcgcttg atttccattc
     3721 tttccccgga tcagttgttt cgcgaggatg tcatgaagcg attggcaggt gcgcgacagg
     3781 catccagcac gccggccctg caggccgaca gcggcgcgcg cgaagaaatg agcttcgtgg
     3841 ttttccggct ggggagcgag gaattcgggc ttccgatcga tacggtcgtc gaggttgctc
     3901 aggtcccgca gcaaatcacc cgcgtaccca aaacgccgaa gttcctcgag ggtgtcgtca
     3961 atcttcgcgg cgaggttctg ccggtggtgg accaacggcg ccgcttcgag atgccgaagc
     4021 tcgataacgc cgaggggcgc aggctcgtcg tcatcaggac cgagcgccat cgcgcaggcc
     4081 tcatagtcga tagcgtctcg gaagtcttgc gaacgaccgc cgacagcgtc gagcctcctc
     4141 ctgagctgac tgatcaggtc tcgcgtctgg tgcgaggtgt catcaatctg ccgaagtcca
     4201 accgcatggt gctgctgctg gatgctgccg agctcctcac gcgcagcgaa caaggcctgc
     4261 tggactcttt tcaggcgaag agcaagaagg cgaatgagtg atcagggttc tcgtggtcga
     4321 cgactctgcg ctcgtgcgca agctcttcgg gcgagtgcta tcgggagagc cggatttcgc
     4381 ggtgacattt gcgcgcgacg gactcgaggc gctcgctcag ctcgaagcgt tcaggccgga
     4441 tgtcatcacg ctggacgtgc aaatgcctca gatggatggt ctcgagtgcc tggatcgaat
     4501 catggtccag cggccctctc cggtggtgat ggtgtccacg atgaccgcgg aaggcgcgga
     4561 ggcgaccttg gatgccctga ggctgggcgc tgtcgacttc gtaaccaagc cggccggggc
     4621 ggtgtcgctg cgcatcgatg agttcgctcc gacactcaag cagaaaatcc gtgccgctgc
     4681 aggggccagg cttccggcca gccgccgtct caaggagcgc gtgcaattca gagccggcgc
     4741 tacgcccacc cggaaaccgc gcaagctcga agtccccgag gagcacatcg cagccattca
     4801 aggcgcggga atcgtgctcg tggggacctc cacgggcgga ccgcccgctc tggaggctct
     4861 gctggtccaa cttccagcca gctttccctg gccgatcctc gtcgcgcagc atatgccagc
     4921 cagcttcacc gggccgctgg ccaagcggct ggatggaatc tgcccactcc aggtgatcga
     4981 ggcggcccaa cccatgccgt tgcaggccgg ctgcgtgtat atcggccggg gcgatgccga
     5041 tctcctcgtg tccaaacggc catcgggctt gatcgtgagg gcggtcccgg cgaaagccga
     5101 ttatccctgg catccgagca cggaccgcct ggttcgatcc gccatggagc acgtgccggc
     5161 cagtcaactc atcggggtgc tgatgacggg catgggaaat g
//
LOCUS       DASRHN010000228        12850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_744481, whole genome shotgun sequence.
ACCESSION   DASRHN010000228 DASRHN010000000
VERSION     DASRHN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12850
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744481"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFB37_08760"
     CDS             complement(<1..781)
                     /locus_tag="VFB37_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZO22583.1"
                     /translation="MAAARPQSMMAMRLHSRGTALAVDEVPCPAPEEYQVLMQVLACG
                     VCRTDLHLRDGELPELQYPITPGHEIVGRIVERGQSVAGLEVGMRVGVPWLGYTCGIC
                     RYCRKGLENLCERARFTGYSMDGGYAEYAIADARYCLALPERYSDLEAAPLLCAGLIG
                     YRAYRAAGEAQNLGMYGFGAAAHLLTQVARADRRRVFAFTRPGDENFQAFARTLGAVW
                     AGSSDEVPPELLDAAIIFAPIGALVPAALQAVRKGGRVVCAG"
     gene            1311..2618
                     /locus_tag="VFB37_08765"
     CDS             1311..2618
                     /locus_tag="VFB37_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HZO22584.1"
                     /translation="MSVHTLRVLDSTANVGTIDQESFDERFSFRYSPQWLREPGSYSI
                     SPHITVLGPDAGSGTIRRFLENLLPEGPALEALSVAHRVPRNNPCGLIRACGWETAGA
                     LSFVSREVEPSDEPGREVTPKELAQRIRGRPRSPFSVWDGRVRVSIAGAQDKLPVYQD
                     HDGRMYLPAWKLASTVILKPEPVDVRLPMLVANEHFCMSLARKLELPAATVSILRVPE
                     PILMVERFDRMRDAQGVRRRHVVDGCQALNLPVSCKYEHNITLGQDSAERREGVSFER
                     LFSIADYSVEQEATRLMLLRWALLQFLIGNTDAHAKNVSFFCGQSGLVLAPYYNLASL
                     AQYPGVRQELAMACGDEFVLERIRPYDWAEFAARTGTPRRVLVREMRRLAAAAGPAAV
                     QQASDPVYANEEARFLARIGTFVQAQAARLLEMADLILKVDLE"
     gene            2772..3272
                     /locus_tag="VFB37_08770"
     CDS             2772..3272
                     /locus_tag="VFB37_08770"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HZO22585.1"
                     /translation="MNPVQKIGVALACASLVIAGSALAQPDREHGGAHPPEGRAEHAP
                     GGEHRGEAHPGPRGYQRVGPPHGWDARPKTVDRAAYRHNFRAARSYRIGPYHRPAGWA
                     PHHWAYGEVLPRAYWGPDYLIGDYWLFALEVPPAGYEWVRDDADALLVDTNTGEILQV
                     EYGVFA"
     gene            3390..5189
                     /locus_tag="VFB37_08775"
     CDS             3390..5189
                     /locus_tag="VFB37_08775"
                     /inference="COORDINATES: protein motif:HMM:NF042569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HZO22586.1"
                     /translation="MTKNAAVEYAYARLVACTQEDARVCATVDLILAIFDEFYAQFCE
                     YPYRAKRAFETMDPHASLRISRERLGLYSRYVEEHGPRIVAAVPALSNNAALWDAIDH
                     LLLAMIAERYEADIIFGFTHSLRRNIDHGIWRPVAYAFPPPSKRRVDSMAHVHRRLPI
                     RGHIDAALLTEALQIPGFSVPFRDLKADTHRVLQRLEQLLHSTPDAPLPVALDVVEAG
                     FFRDRTAFLVGRWVMTDATYVPFVVAFLNSPSGIHVDAVLHQVADIHGLFSSALANFH
                     VTTRLYYQTCVFLFSLMPRRPLGHHYATIGFNHVGKVVILEEIIEQSRSSGEKLRRSP
                     GYPGTVALGFSFDSCSYHLKVIRDRPTSSYKWGTFPGITAVVDKYRQVHEMNRAASML
                     DNVMYFNVRLDRDLFEPGLLEEIFQQTAGSVHLDGDGVFFRFLIVQLRIVPLPVYLQQ
                     ANEAETHAVIRSLGHCIRNNAATNIFNKDLDSRNYGVGRYGRVFLFDYDAVEKLTDVK
                     IRTNAGREPGEETPPDWFWEDGVIFLPEELEHGLLLNSDLARRSFHRQNADLLSVQYW
                     EEVQQRLQRGEVPEVRMYPRAAKLPGNARLARE"
     gene            5186..7090
                     /locus_tag="VFB37_08780"
     CDS             5186..7090
                     /locus_tag="VFB37_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22587.1"
                     /translation="MSAAPDTPQGPYGPWNPGIDSQLPVSLRSLCTILRPDNVTTTLA
                     AASELQQLTGFAATEIVAFRPERLLLHELLIRVTGDFSVPDGSRIEDLGINFREMTGL
                     LLRRYLQPRMGEVTEVFAQVRRELAQSIESALAGLATHASSVRTPVRQGRLRRLARKW
                     RERRTGADSRAEVMPPRQDKSLTWDPRHIVECERLASRAEDPLHAAGYRSLARVMAAL
                     FTTHGRPWGTPQLIVSLATDMACNVQGGDAIGRLIEPILKQAATHEGYKLLPRQERPI
                     VINTKGPSASGKSTLRPLQRKLAGEIGARWSDFALISPDIWRKQLLDYGSLGGHYKYA
                     GALTSEELQIVDQKLDRYMALKQRRGDMAHLLIDRFRFDSFAPDSDEAGSNLLTRFGR
                     LVYLFFMITPPEMLVERAWKRGLQVGRYKAVDDTLAHGVEAYTGMPDVFFTWVRRTDK
                     RIRFEFLDNSVRLGERPRTVAFGTNSIFNALDVGGILNIERYGRVNVEAKEPRALYPD
                     RSLLAAEHNLSFLRRCVAGFREVNFAEHATGRVYLRIEHGTPVRVDRQALQAAVVDPC
                     TLAGVRAIAPTALEGVVPAVSEPEYLPGSGSSPAERLNTAVATSRAERSLTLGQWGAT
                     RLEGLREPDD"
     gene            7550..8881
                     /gene="oxlT"
                     /locus_tag="VFB37_08785"
     CDS             7550..8881
                     /gene="oxlT"
                     /locus_tag="VFB37_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HZO22588.1"
                     /translation="MSVDVKAVGTHDPMQNTGVQNRWWQLVIGIICMAMVANLQYGWT
                     LFVNPMHEKFGWTKAAIQVAFSVFVLCETWLVPVEGWLMDRFGPRPVVLVGGILAGIS
                     WILNSYASSLGMLYFSAVIGGIGAGAVYGTCVGNALKWFPDKRGLAAGLTAAGFGAGA
                     ALTVIPIANMVKSGGYDRAFFDFGLIQGIVVLVLSVFLIRPPTSATVVVKAGAVNPLS
                     RRNFGPGEMLKTPVFWVLYVCMVLVACGGLMAAAEVAPIAKDYGVDKIPINYFGAILP
                     LLTVALSIDSLVNGLTRPLTGWISDHLGRENTMVLVFAVEGLAIWGLMKFGHSAGGFL
                     LFAPLIFLGYGEIFSLFPAASGDTFGPKYAATNNGLLYTAKGTSSLLIPLANVLKASA
                     GSWVPVLWIAGALPIIAAVLSKAVLAPMRRRMLERVAAEVPDRPLGQTVQG"
     gene            complement(8958..9698)
                     /locus_tag="VFB37_08790"
     CDS             complement(8958..9698)
                     /locus_tag="VFB37_08790"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO22589.1"
                     /translation="MAELARLMNTTARLLQRASSSTPLALAPLNATSLRDQAYALLKT
                     AIADTDIYNPDQELRLDERQLTTALGVSRTPIREALSLLEQEGFIRTVPRRGIYIIRK
                     SKRQIIEMIQMWAALESMAARLATLHAPDSEIARLRHLFDEFQNSPPSEHLGEYSDAN
                     IAFHTEVIRLGGSQMIIDTTRNLLLHVRAIRRATISQSDRASRSIIDHLKIIEALERR
                     DTELAERLTRQHTLDLASYVDRYCDFLD"
     gene            10445..12205
                     /locus_tag="VFB37_08795"
     CDS             10445..12205
                     /locus_tag="VFB37_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZO22590.1"
                     /translation="MATVNPTPVQSKTTTAEDTLQKKSREAGVVSGGHLVAKALKNEG
                     VDTIFTLCGGHIIDIYDGCIDEGIRIIDVRHEQVAAHAADGYARQTGKLGCVVTTAGP
                     GCTNAVTGVATALRSESPILHIGGQGALTQHKMGSLQDLPHVDMMSPITKFAATVPST
                     ERVADMVSMAAREAFNGAPGPVYLEIPRDVLDREVDLSKASIPKPGRYRASTRSIGDP
                     RDIERLADILVNSERPAILYGQQVWTARGHTEAIALLRGLDIPGYFNGGSRGLLPPGD
                     PHHFDRTRSTAFAEADVIVIVGTPFDFRMGYGRRISTKLTLVQIDMDYRTVGKNRDID
                     LGLVGDPGAILGAVTQAATGRLKQDKRQARQQWMKKLQAAEATATEKLMPLFKSDNTP
                     IHPYRVAYELNEFLADNTIYIGDGGDVVTISAQAVRPRRPGQWMDPGALGSLGVGTGF
                     AIAAGLAHPNKEVLCYYGDGSFGMTGFDMETANRFGVPYIAVIGNNSAMNQIRYGQLA
                     KYGEQRGNVGNLLGDVPFGKFAEMLGGYGEEVRDPAQIAPALRRARESVQRLRRSAVI
                     NIWVDPREYAPGTKNQTMYK"
     gene            12222..>12850
                     /locus_tag="VFB37_08800"
     CDS             12222..>12850
                     /locus_tag="VFB37_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZO22591.1"
                     /translation="MGKALDGVRILDFTHVQSGPTCTQLLAWFGADVIKVERAGSGDV
                     TREQLQDIPDVDSLYFTMLNHNKRSITLDTKSEPGKEILEKLVKRCDVLVENFAPGAL
                     DRMGFTWERIQQLNPRMIVASVKGFGPGPYEDCKVYENVAQCAGGSASTTGFDDGPPL
                     VTGAQIGDSGTGLHLALGIVCALYQRNRTGRGQKVLAAMQDGVLNLCRV"
BASE COUNT         2305 a         3965 c         4161 g         2419 t
ORIGIN      
        1 tgccagcaca aaccacccgg ccacccttgc gcacggcctg cagggcagcg ggcaccagcg
       61 cgccaatcgg agcgaagatg atcgccgcat ccagcaactc cggcggcact tcatccgagc
      121 tgccggccca aaccgcaccc agcgtgcgtg caaaggcctg gaagttctca tccccgggac
      181 gggtgaatgc gaacactcgg cgtcgatcgg cgcgtgcgac ttgggtgagc aggtgcgccg
      241 cggctccgaa accgtacatg cccagatttt gcgcctcgcc cgccgctcga taagcgcgat
      301 agccgatcag tccggcacaa agcagtggtg cggcttcgag gtcggaatag cgctcgggca
      361 gcgccagaca ataccgcgca tcggcaatgg cgtactcagc gtagcctcca tccatggaat
      421 agcccgtgaa tcgagctcgt tcgcacaggt tttccagacc cttgcggcag tatcggcaga
      481 tgccgcaggt gtatccgagc cacggcacgc ccacgcgcat gccaacctcc aggcccgcca
      541 cactctgtcc acgctcgacg atacggccga cgatttcgtg gccaggcgtg atcggatatt
      601 gcagctcagg aagctcgcca tcgcgaagat gcaggtccgt gcggcagacg ccgcaagcga
      661 gaacctgcat caatacctga tactcctcag gagcgggaca aggcacctca tccaccgcga
      721 gcgccgtgcc ccgcgaatgc agccgcattg ccatcatgga ctgtgggcga gcagccgcca
      781 cgccacgctc ctcgtctcga tccgcgcgcg atcgggactt gcgctgttga ggtctcgaat
      841 tctcagtcca cttcagtgct caagcaagct aagcgcggga gtcggtgaag cgccctggcc
      901 gcgatcaagg agatcggctg catcctggct tgagattccc ggtcgcgcgc gatcgaaggc
      961 catgaggttg ctccgatccc gggctcgagg ccgagcgaat cttggtgcgc gcctcaatgt
     1021 gctccgttgc cgcgcgccat gagcgcaaaa ttgttggaag gaaagccggg gaaagggcgg
     1081 caatgcggct gcccgcgcgg gcactgcaaa gggagccggg agctcgtcgg gcgtgaccct
     1141 tccggcggtg ccttgataat caggaactta cacgatcgac caaacccgcc gtaatctgat
     1201 cgggttcgcc tggacttgcg ggctcaaaac cgggggccgc gagcagtgct gccgatccgt
     1261 aagggatcag gggcccgctt gcggcgtgtc acgaggacaa cctgctcgct atgagtgtgc
     1321 atacgctacg cgtgctggat tcgacggcga acgtcggcac catcgatcag gagtcgttcg
     1381 atgagcgctt ctcctttcga tactcgcctc agtggttgcg tgagcccggc tcgtactcga
     1441 tctcgcccca catcactgtg ctcggccccg atgcgggatc gggcaccatc cggcgattcc
     1501 tggagaatct ccttccagaa ggccccgcac tcgaggcgtt gtccgtcgcg caccgggtgc
     1561 cccgaaacaa tccttgcggc ctgatccgcg catgcgggtg ggaaaccgcc ggcgcactct
     1621 cgttcgtgag ccgggaggtc gaacccagcg atgagcctgg cagggaagtg actcccaagg
     1681 agcttgcgca gcggatccgc ggacggcccc gctcgccttt ttcagtctgg gacggtcgcg
     1741 tgcgtgtatc gatcgccggg gcgcaggaca agctgccggt gtaccaggat cacgacggcc
     1801 gcatgtacct gcctgcctgg aaactcgcct cgacggtcat tctcaagccg gaacctgtcg
     1861 acgtgcgcct gccgatgctg gttgccaacg agcacttctg catgagtctg gcacgcaaac
     1921 tcgagctccc ggcagcgacg gtatccatcc tgcgagtgcc cgagccgata ctgatggtcg
     1981 agcgattcga tcggatgaga gacgctcaag gtgtgcggcg gcggcacgta gtggatggct
     2041 gccaggctct caacctcccg gtctcctgca agtacgagca caacataacc ctggggcagg
     2101 acagcgccga gcgccgcgag ggtgtcagct ttgagcgcct gttctcgata gccgattaca
     2161 gcgtcgagca ggaggccacg cggctcatgc tgttgcgatg ggctctactg cagtttctga
     2221 tcggcaatac cgatgctcac gccaagaacg tctcgttctt ctgcggtcag tccgggcttg
     2281 ttctggcccc gtactacaat cttgcgagtc ttgcgcagta ccctggagtg cggcaggagc
     2341 tggcgatggc ctgtggagac gagtttgttc tggagcgcat caggccttac gactgggcgg
     2401 aattcgcggc gcgcacgggc acgccgcgcc gggtgctggt gcgggaaatg cggcgtctgg
     2461 ctgctgccgc cgggccggcc gccgtgcagc aggcaagcga tccggtgtac gcgaacgaag
     2521 aggcgcgctt ccttgcccgg atcggcacct tcgttcaggc tcaggcggcc cggctgttgg
     2581 agatggcgga cctgatcctg aaggtggatc tggaatgaga ggggcggggt ttgcgccggt
     2641 gcacttccgc ggcggcacgc tggatgtcag ggccgggcac taacattgct ggagcgccgg
     2701 ctcgccgacc gaccgctttc gcaaggcgcg ctcaaaaaat tacatctggg accgagtgga
     2761 ggaattctca cgtgaaccct gtacagaaga tcggtgttgc attggcgtgc gcatccctgg
     2821 tgatcgcggg ctccgccctg gcccagccgg accgtgagca cggcggtgcg catcctcccg
     2881 aaggccgggc cgagcacgct ccgggaggcg agcatcgcgg cgaggcgcac cccgggcccc
     2941 gcggctatca gcgcgtgggt ccgccgcacg gctgggatgc aaggcctaag acggtcgatc
     3001 gagctgccta tcgccacaac ttccgggccg cgcgcagcta caggataggc ccctatcacc
     3061 ggcctgccgg ttgggcaccc caccactggg cctacggtga ggtcctgcca cgcgcctact
     3121 ggggccctga ctacctcatc ggagactact ggctgttcgc cttggaagtg ccgccagccg
     3181 gttacgagtg ggtgcgcgac gatgccgatg cgctgctggt ggataccaac accggggaaa
     3241 tccttcaagt ggagtatgga gtcttcgcct gaaggccgct tgcaggctag catcgggcag
     3301 gcgctctgcc cggatccctt cgttgcgagg tgcgtgtcga gggcgggcag cacgcacctt
     3361 acttccgtcc ctctcggtaa cagatgagcg tgaccaagaa tgccgcagtg gagtatgcct
     3421 atgcgcgcct ggtggcctgc acgcaggagg acgcaagggt ctgtgcgacc gtcgatctca
     3481 tcctggcgat cttcgatgaa ttctacgcac agttctgtga gtatccgtac cgcgcaaaac
     3541 gtgccttcga aacgatggat ccgcatgcat cgcttcgtat cagcagggag aggctcggtc
     3601 tttacagccg ctacgtagaa gagcacggcc cgagaattgt cgccgcggtt cccgccctct
     3661 cgaacaatgc cgcgctatgg gatgcgatcg atcatctgct gctggcaatg atcgccgagc
     3721 gctacgaggc cgacatcata ttcggcttca cgcattccct gcgacgcaac atcgatcacg
     3781 gcatctggcg gccggtagcg tacgcctttc cgcccccgag caaacgccgt gtggactcga
     3841 tggcgcacgt ccatcgccgg ctgcccatcc ggggccacat tgatgctgcg ctgttgaccg
     3901 aagcgctgca gatcccggga ttttcggtgc catttcgaga cttgaaggcc gatacgcatc
     3961 gtgttttgca gcggctcgaa cagctgctgc actccacgcc ggatgctccg ctgccggtcg
     4021 ccctggacgt cgtggaagcg ggttttttcc gtgatcgcac ggcctttctg gtcggccgct
     4081 gggtcatgac ggatgccacc tatgtgccgt tcgtcgtggc ctttctcaac agtccgtccg
     4141 gcatccatgt cgatgcggtg ttgcatcagg ttgccgacat ccacggcctt ttcagctccg
     4201 cgctagccaa cttccatgtc acgactcgcc tctactatca gacttgcgtg ttccttttca
     4261 gcctgatgcc acggcggccg ctcggtcacc actacgcgac gatcggcttc aaccacgtgg
     4321 gcaaggtcgt cattctcgag gagatcatcg agcagagtcg ctcgagcggt gagaagctgc
     4381 gccgctcccc gggctatccg ggcacggttg ccttggggtt cagcttcgat tcctgctctt
     4441 atcatctgaa ggtgatccgg gaccggccga cctcctcgta caagtggggc acctttccgg
     4501 gaatcacggc cgtggtggac aagtatcgcc aggttcatga gatgaaccgc gcggcgagca
     4561 tgctcgacaa tgtcatgtac ttcaacgtcc ggctggaccg ggatctgttc gagcccgggc
     4621 tgctggagga gattttccag cagaccgccg gcagcgtgca tctcgatggg gacggggtgt
     4681 tcttccgctt cctcatcgtg cagctgcgga tcgtcccctt gccggtctat ctgcaacagg
     4741 ccaacgaggc agaaacgcac gccgtcatcc gcagcctcgg acattgcatc cgcaacaatg
     4801 ccgccacgaa catcttcaac aaggacctgg actcgaggaa ctacggtgtg ggtcgctatg
     4861 gtcgggtctt cctgttcgat tacgatgcgg tcgagaagct gacggatgtg aagattcgca
     4921 ccaacgcggg acgtgagccg ggcgaggaga ctccgcccga ttggttctgg gaggacggcg
     4981 tgatttttct gcccgaggag ctcgagcatg gcttgctgct caacagcgat ctcgcgcggc
     5041 gctcgttcca ccgtcagaat gccgatcttt tgagcgtgca gtactgggaa gaggtgcagc
     5101 agcggctgca gcgcggcgaa gtacccgaag tgagaatgta cccgcgcgcc gccaaactgc
     5161 cggggaatgc aaggctcgca cgcgaatgag tgcagcgccc gacaccccac agggaccgta
     5221 cggcccgtgg aatccgggca tcgactcgca gctgccggtc tcgctgcgct ctctgtgcac
     5281 catcctgcgg ccggacaacg tcaccaccac tctcgccgcg gcgagcgagt tgcagcagct
     5341 gaccggcttt gccgccacgg agatcgtggc tttccggccc gagcgactgc tgctgcacga
     5401 gctgctgatc cgcgtgactg gcgatttctc ggtgcccgac gggtctcgca tcgaagacct
     5461 cggcatcaac ttccgggaaa tgactgggct tctgctgagg cgctatctgc agccccgcat
     5521 gggcgaggtc accgaggtgt tcgctcaggt ccggcgggag ctcgcgcaga gcatcgaaag
     5581 tgccctcgca ggccttgcca cgcatgcctc gtccgtgcgg acaccagtgc ggcaaggtcg
     5641 cttgaggcgg ctcgcacgca agtggcgtga gcggcgcaca ggcgccgatt ctcgcgccga
     5701 ggtgatgccg ccacggcagg ataagtctct cacgtgggac ccgcggcaca tcgtcgagtg
     5761 cgagcggctg gcaagccgtg cggaggatcc cttgcacgcg gcaggctatc gcagcctggc
     5821 gcgagtcatg gccgccctgt tcaccacgca tggacgaccc tggggtacgc cgcagctgat
     5881 cgtgtccttg gccacggata tggcgtgcaa cgtccaggga ggggatgcca tcggtcgcct
     5941 gatcgagccc atcctgaagc aggcggcaac gcacgagggc tacaagctgt tgccacggca
     6001 ggagcgcccg atcgtgatca acacgaaggg accctcggcc tcgggcaaga gcactctgcg
     6061 gccgctgcaa cgcaaacttg ccggcgagat cggcgcgcgc tggagcgact ttgccctgat
     6121 cagtccggat atctggcgta agcagctgtt ggactacggc tctctgggcg gccactacaa
     6181 gtatgcgggg gcgctcacct ccgaggagtt gcagatcgtc gatcagaagc tcgatcgcta
     6241 catggcgctg aagcagcgcc gcggcgacat ggcgcacctg ttgatcgatc gctttcgctt
     6301 cgacagcttt gcgccggact cggacgaggc gggcagcaac cttctgaccc gtttcggccg
     6361 attggtctat ctgtttttca tgatcacccc gccggagatg ctcgtggagc gagcctggaa
     6421 gcgcgggctg caagtcgggc ggtacaaggc ggtcgacgat acgctggcgc acggagtcga
     6481 ggcctacacg ggaatgccgg atgtgttctt cacgtgggtt cggcgcaccg acaagcgcat
     6541 acgcttcgag tttctcgaca attcggtgcg gctgggcgag cgaccgcgta cggtcgcctt
     6601 tggaacgaac tcaatcttca atgcgctcga tgtcggaggg atcctgaaca tcgagcgcta
     6661 cgggcgggtg aatgtcgaag cgaaggagcc gcgcgctttg taccccgacc gcagtctgct
     6721 cgcggcggag cacaacctca gtttcctgcg tcgttgcgtc gcgggcttca gggaagtcaa
     6781 cttcgcggag cacgccaccg ggcgtgttta tctgcgcatc gagcacggca ctccggtgcg
     6841 tgttgacagg caagcgcttc aagccgcggt ggtggacccg tgcacgctcg caggtgttcg
     6901 cgcaatcgca ccgaccgcgc tcgaaggggt ggtcccggcc gtgtcggagc ccgaatatct
     6961 gccgggctcc ggcagctcgc cggcggagcg cctaaacact gcggttgcga caagccgtgc
     7021 ggagcgcagc ctcaccctcg ggcaatgggg agcaacgagg ttggagggac ttcgcgagcc
     7081 ggacgactga tccaatggcc aaacccgaag tgggtgttcg actggcgatt gaatgcgcgg
     7141 catgcgcatc gtctctgccg cgtgcgcagc gtgcgtggaa accggagttg aggccgccat
     7201 cttgatcgcg gctagagctc gtcaatgtgt tagcgagccg gaacagtgct cgcttgactt
     7261 tccagatttt tggacatata tcatatacta gcaagagaaa aacagacgat ccggctccgg
     7321 gggccagtag aggggttacg gtgagaagcc ttcattcctg tgcgcactct gtgcggacaa
     7381 gttctttgtc tctcagtaac gggcaccgcg ctgacggcga gctgagtgtc tttgctcagc
     7441 gccgcagacg tcctcgcatt cgattctcat tcgattctgg cggcacgttt ttcgcgcccg
     7501 ccgtgctgat ctctaaacct cactcaggtg aagaataaag gggaaggata tgagcgtcga
     7561 cgtgaaggca gtcgggacac acgacccgat gcagaacacc ggggtgcaga accgctggtg
     7621 gcagctcgtc atcggcatca tctgcatggc gatggtggcc aacctgcaat atggctggac
     7681 gctgttcgtc aatccgatgc acgagaaatt cggctggacg aaggccgcga ttcaggtcgc
     7741 gttttcggtc ttcgtgctgt gcgaaacctg gctggtgccg gtcgaaggct ggctgatgga
     7801 ccggttcggc ccgcgcccgg tggtgctcgt gggcggcatt cttgccggga tctcgtggat
     7861 cctcaactca tacgcgagct cgctcggcat gctgtacttc tccgcggtga tcggcggaat
     7921 cggcgcggga gccgtgtatg gaacctgtgt cggcaatgca ctcaagtggt ttcccgacaa
     7981 gcgcggactt gccgccgggc tcacggctgc gggcttcggc gcgggcgctg cgctgaccgt
     8041 gatccccatt gccaacatgg tgaaatccgg cggctacgac cgagcgttct ttgacttcgg
     8101 actgatccag ggcatcgtcg tcttggttct gtcagtattc ctgatccgcc caccgacgag
     8161 tgccacggtg gtcgtgaagg ccggggcggt gaatccgctc agccggcgta atttcggccc
     8221 cggcgaaatg ctcaagaccc cggtgttctg ggtgctctat gtgtgcatgg tgctggtggc
     8281 ctgcggtggc ttgatggccg cggcagaagt ggctccgatc gccaaggact atggcgtaga
     8341 caagatcccg atcaactatt tcggcgccat cctgcccctg ctcaccgtcg cgctctcgat
     8401 cgatagtctg gtcaacggcc tgacacgacc gttgacgggc tggatttccg accacctcgg
     8461 gcgcgagaac accatggtgc tggtcttcgc ggtcgaaggg ttggccatct ggggcctgat
     8521 gaagttcggc cacagtgcgg gaggcttcct gcttttcgca ccgctgatct tcctcggcta
     8581 cggcgaaatc ttctcgctgt tcccggctgc gagcggcgac acgtttggac cgaaatacgc
     8641 ggcgactaac aatggcttgc tctacacggc caagggcacc tcgtcgctgc tcattccgct
     8701 ggcgaacgtc ctcaaggctt ccgccggcag ctgggttccg gtgctctgga tagcgggcgc
     8761 gctacctatc atcgccgctg tgctgtcgaa ggccgtgctg gcacccatgc ggcgacgcat
     8821 gctcgagcgt gtggctgccg aggtaccgga tcggccgctc gggcagaccg tacagggctg
     8881 aggacattca gcttgcagaa agaagaaggg gcggcccggc cgccccttct tgcatccggg
     8941 gtgcttctcg cgcgtcgcta atcgaggaag tcgcagtagc ggtccacgta gctggccaga
     9001 tccagggtgt gctggcgcgt cagccgctcc gcgagctcgg tgtcccgcct ttccagcgct
     9061 tcaatgatct tcaggtgatc gatgatcgag cgcgacgcgc gatcgctctg gctgatcgtg
     9121 gcccgccgga tggcgcgcac gtgcagcaaa agattgcgcg tcgtatcgat aatcatctgc
     9181 gagccgccca ggcggatgac ctcagtatgg aatgcgatgt tggcgtcaga gtactcaccc
     9241 aggtgctccg agggcgggga gttctgaaat tcgtcgaaca gatgccgcag ccgggctatc
     9301 tccgagtcgg gcgcatgcag ggtagccagt cgcgcggcca tcgactcgag ggctgcccac
     9361 atctggatca tctcgatgat ctgccgcttg gatttgcgga tgatgtaaat gccgcggcgc
     9421 ggcacggtgc gaatgaaacc ctcctgctcc agcagcgaga gtgcctctcg gataggtgtt
     9481 cgactgacac ccagggccgt cgtgagctgc cgctcgtcca gccgcagctc ctgatctgga
     9541 ttgtaaatgt cggtatcggc gatagccgtt ttcaggagtg cgtaggcttg gtcacgaagg
     9601 ctggtcgcat tgaggggggc gagagcgagc ggtgtcgagg aggaggcccg ttgaagaagt
     9661 cttgccgtgg tgttcattag gcgcgcgagc tcagccattg gatgagatat atgagattat
     9721 acgatacccg atgcttgatc taggcgcacg ggtgcggaac gggcgggtgc cggcacctcg
     9781 ggcggggtgt tggcacctgc gctgccggtc gaaacccact cgatgggctg aataggcgcg
     9841 ccgttgttgt agcgacgcca cgcttcgcat cctgacgttc gtcgtggctt tcagtcttgt
     9901 cttgcaaggc tcagcgacaa atcttccgca cggtccgagt acccgctgtg ctcgaaacgg
     9961 aacagatccc acttgacatt aaatttttca gaaatatatg atataccaga agaagaaata
    10021 cgggatcgac gggcggcgcg gagagagtca ggactgcttg gggatgtggt tgagggggaa
    10081 gagtgagcga atctcacgat gttcgcactt tgtgcgaacg agtgctccgt tccgccgccc
    10141 ccgcgcggga cgcccgcctc gttgactcaa ttttcgttga ttcaaaccac gctggcctgc
    10201 cggctcgccc tcgcacacgt catccgtgcg cggatgtgcg ccgaccactc gcgcacctgg
    10261 ggccctgcaa cgtgggggaa gctgatgccc cctgccggca aaaattcggg cttcgccccg
    10321 gaatgccggc tcacgccacg gctgacggtg ccgcgatgca ccgggcctgg agaatatggt
    10381 cggccggctc cgatgcggca tcgccgcgac cccggccaac cgagatcacc ttgctggagt
    10441 tgatatggca accgtaaatc ccacgcccgt gcagtcaaag accacgaccg ccgaggacac
    10501 cctgcagaag aagagtcgcg aagccggtgt tgtttccggt ggccacctgg tcgccaaggc
    10561 gctcaagaac gagggcgtcg atacgatctt cactctgtgc ggaggtcata tcatcgacat
    10621 ctatgacggc tgcatcgatg aaggcattcg catcatcgat gtgcgccatg agcaggtcgc
    10681 cgcacacgcc gctgatggct atgcgcgcca gacgggcaag ctcggttgtg tcgtcacgac
    10741 ggctggaccc ggttgcacga atgctgtgac gggcgtggcg actgcgctgc gctcggaaag
    10801 cccgattctg cacatcggcg gacagggcgc gctgacccag cacaagatgg gctcgctgca
    10861 ggatctgccc cacgttgaca tgatgtcacc gatcaccaag ttcgctgcca ccgtgcccag
    10921 caccgagcgc gtggcggaca tggtctcgat ggccgcgcgc gaggcgttca acggcgctcc
    10981 gggcccggtg tatctggaga tcccgcgcga tgtgttggat cgcgaagtgg atctgtccaa
    11041 ggccagcatc ccgaagcccg gccggtatcg cgcttcgacg cgctcgatcg gcgatccgcg
    11101 cgacatcgag cggctcgccg acatcctcgt caactccgag cggcccgcga tcctctatgg
    11161 acagcaggtc tggacggcac gcgggcacac cgaggcgatc gcgcttctca gaggtctgga
    11221 cattccgggt tacttcaacg gcggcagccg tggactgctt ccccctgggg atccgcatca
    11281 cttcgaccgc acgcgcagca ccgcctttgc cgaagccgac gtgatcgtca tcgttgggac
    11341 gccgttcgat ttccgcatgg gctatggacg gcgcatcagc acgaagctga ctctcgtgca
    11401 gatcgacatg gattaccgta cggtcggcaa gaaccgtgac atcgacctcg ggctcgtcgg
    11461 cgatccgggc gccatcctcg gtgccgtgac gcaggcggcc accggccgtc tcaagcagga
    11521 caagcgccag gcgcgccagc agtggatgaa gaagttgcag gctgccgagg cgactgcaac
    11581 cgagaagctg atgccgctct tcaagtccga taacacaccc atccatccgt atcgcgtcgc
    11641 ttacgagctc aacgagttcc tggccgacaa cacgatctac atcggtgacg gcggtgacgt
    11701 ggtgaccatc tcggcgcagg ccgtccgccc gcgccgcccg gggcagtgga tggatccggg
    11761 tgcgctgggc tcactcggcg tcggcaccgg tttcgcgatt gccgccgggc tcgcgcatcc
    11821 gaacaaggag gtgctgtgct attacggcga tggctcgttc ggcatgacgg gcttcgacat
    11881 ggagacggca aatcgctttg gcgtaccgta catcgcggtc atcggcaata actccgcgat
    11941 gaaccagatc cgctacggac agcttgcgaa gtatggcgag cagcgcggca acgtgggcaa
    12001 tctgctgggc gatgtgccct tcggcaagtt cgccgagatg ctcggtgggt atggcgagga
    12061 ggtgcgcgac ccggcacaga tcgcgccggc actgcgccgg gcacgcgagt cggtgcagcg
    12121 tctgcgtcgc tctgccgtga tcaacatctg ggtagatccg cgggaatacg cgcccggaac
    12181 caagaatcag accatgtaca agtgatggtc cggagactcc catgggcaag gcattagacg
    12241 gcgtccgcat cctggacttc acgcacgtgc agtcggggcc gacctgcacg caattgcttg
    12301 catggttcgg cgccgacgtg atcaaggtgg agcgggcagg ttcgggtgat gtcacgcgcg
    12361 agcagctgca ggacatcccg gatgtcgaca gcctctattt caccatgctg aatcacaaca
    12421 agcgctccat caccctcgac acgaagagcg agcccggcaa ggaaattctg gagaagctgg
    12481 tcaaacgctg cgacgtactg gtggagaact ttgcacccgg cgccctcgat cgcatgggct
    12541 tcacctggga gcgcatccag cagctcaacc cccgcatgat cgtggcctcg gtgaagggct
    12601 ttgggcccgg gccatacgag gattgcaagg tctacgagaa tgtcgcgcaa tgcgccggcg
    12661 ggtctgcttc gaccaccggc ttcgatgacg gtccgccgct ggtgaccggg gcgcagatcg
    12721 gcgacagcgg cacggggctg catctggcgc tcggcatcgt ctgcgctctg tatcagcgca
    12781 atcgtaccgg ccgcggccag aaggtgctgg cggccatgca ggatggcgtg ctgaacctgt
    12841 gtcgcgtcaa
//
LOCUS       DASRHN010000229        28083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2382680, whole genome shotgun sequence.
ACCESSION   DASRHN010000229 DASRHN010000000
VERSION     DASRHN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 28083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28083
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382680"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFB37_08805"
     CDS             complement(<1..710)
                     /locus_tag="VFB37_08805"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HZO22592.1"
                     /translation="MALPIALYSTAQVRALDAHAIETLGVPGYTLMKRAGEAALRYLR
                     TRWPTAHRIVIVCGGGNNAGDGYVLGRFAQASGLTVCVLAAVSTEALRGDARTACEDF
                     KASEGQLRPFSPELLHEGEVIVDALLGTGLQGSTRPELERVIQEINTAGRPIFALDVP
                     SGLNSDTGIPLGATVRADCTVTFVGLKTGLFIGDGPEFAGTIFFDDLEVSAPAASAFQ
                     PRLERIAEGEILRALPHRR"
     gene            743..1822
                     /gene="queG"
                     /locus_tag="VFB37_08810"
     CDS             743..1822
                     /gene="queG"
                     /locus_tag="VFB37_08810"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZO22593.1"
                     /translation="MSEPRILDLQAAKRALAEGARALGFSRLGVAGVTIPEDEQHLVR
                     WLDAGFEGEMGYMRRHGTLRSRPHELSPGAVRVVSVRMDYWPGDAADAQRTLQDPTLG
                     YVSRYALGRDYHKIMRRSLARLAEQLRLRIGPFGYRVCVDSAPVLEKAFARNAGLGWI
                     GKHTNLIAQEAGSWFFLGEILTDLPLPLDEPASAHCGSCEACIPACPTGAIVGPYRLD
                     ARRCISYLTIEHASAIPEALRPAIGNRIYGCDDCQLVCPWNKFARDAAHPDFKVRHRL
                     DATHLSALFAWTPAQFEERMRGSAIYRIGYERWSRNIAVALGNAPSSTEVLTALRSRS
                     TDSSAMVREHVEWALARHRHEREPH"
     gene            complement(1833..2681)
                     /gene="panC"
                     /locus_tag="VFB37_08815"
     CDS             complement(1833..2681)
                     /gene="panC"
                     /locus_tag="VFB37_08815"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZO22594.1"
                     /translation="METVTSVATVRERVRAWHAAGLRVAFVPTMGNLHPGHVSLIEAA
                     RQVGDRFVASIFVNPMQFGPNEDFAHYPRTPRDDERMLADAGCDLMFMPDVTEIYPSG
                     AERATRVEVPALSRILCGEFRPGHFEGVTTVVAALFHIVQPDVAVFGEKDFQQLTIIR
                     RMVADLCFPVRIVGAPTVRDADGLAMSSRNQYLTEQQRQLAPRIHATLSAVAARLQAG
                     DNEFASMERFGVQTLEGAGFRPDYFAIRQAANLAAPEPRTRHLVVLTAAHLGKARLID
                     NIQVER"
     gene            complement(2696..3523)
                     /gene="panB"
                     /locus_tag="VFB37_08820"
     CDS             complement(2696..3523)
                     /gene="panB"
                     /locus_tag="VFB37_08820"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZO22595.1"
                     /translation="MYTHLQLRGSDRPPVTLSTLAQMKADRHKIASLTCYDASFAVLL
                     DNADVDVVLVGDSLGMVIQGYDTTVPVTMDHIVYHCRAVARGLHRPFLIGDLPFMSYP
                     SREQALANAVRLMQEGGAKMVKLESGALQIEIVEFLAAHDIAVCAHLGLKPQSVHKTG
                     GFRVQGRSDDAAERMIENAKRLESAGADIVLLECIPAALGRAVTEALHVPVIGIGAGP
                     DTDGQILVIYDILDITTGRKPRFVRNFMEGVNTNQQALANYVQAVRTGEYPAPEHCF"
     gene            complement(3583..4248)
                     /locus_tag="VFB37_08825"
     CDS             complement(3583..4248)
                     /locus_tag="VFB37_08825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZO22596.1"
                     /translation="MSTKPADPEHRFIVVEGPIGVGKTSLAQRLAASLSAQPVLEQAA
                     QNPFLERFYRNPRAGALPAQLYFLFQRAQQLGGLKQQDLFAPVRVADYLLEKDRLFAR
                     VTLDEAEYGLYEQVYAKLDIQPPKPDLVIYLQAPVDVLLERIGKRGIDYEKSIERQYL
                     ERLNEAYARFFHEFKAAPLLIVNAAAIDPINNQADYDELLGVIKRMKRGRLYFNPPRS
                     RGL"
     gene            complement(4245..4745)
                     /gene="folK"
                     /locus_tag="VFB37_08830"
     CDS             complement(4245..4745)
                     /gene="folK"
                     /locus_tag="VFB37_08830"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZO22597.1"
                     /translation="MDLWRPAYIGVGSNLEDPRAQVLRACLALERIPATRLIRCSPLY
                     GSVPLGAVRQPDFVNAVAGVLTQLEPHALLAELRAIEAASGRPERHPKWGPRVIDLDL
                     LVFAGQRLSGPELTVPHPGVVDRNFVLYPLADIAPDLDVPGLGRVTELKARVGSTGLH
                     LLQRAA"
     gene            complement(4757..6211)
                     /gene="pcnB"
                     /locus_tag="VFB37_08835"
     CDS             complement(4757..6211)
                     /gene="pcnB"
                     /locus_tag="VFB37_08835"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZO22598.1"
                     /translation="MSRAHVSPNALRVLYRLRDAGYMAFLVGGCVRDLLIGIEPKDFD
                     VATDALPEQVRKLFRNCRLIGRRFRLAHVFFGREIIEVATFRAATAPAPGEEPEGEDR
                     DADEAVLEAAALDESDLDEELQSGEPDNGASSGAEAGTAGTDGAERLLDESTERLFDE
                     TGRILRDNIYGTIDADVWRRDLTANALYYNIADFSLWDYVGGFEDIAARKLKLIGDPD
                     TRFREDPVRMLRTARFEAKLGFEIDAATAEPIARLRTLLRGVPPARLFDETLKLFLTG
                     HGRRSFEVLRHRGLLASLLPTVDKYLNTHPDSVVEKLLVQGLENTDARVGADKPVTPT
                     FLFALLLYGPIAGIIESAPAERWHELATILDACDRATREVQSHISLPKRFSLGMREMF
                     ALQPRLEHPRGRRALRVLEHPRFRAAYDLLLLRAQHGMASADIAQWWTRVQEASVEER
                     GRMADALTGHAQRPEGPRRGGRRRRRRRGPSAPA"
     gene            6364..6439
                     /locus_tag="VFB37_08840"
     tRNA            6364..6439
                     /locus_tag="VFB37_08840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6398..6400,aa:Glu,seq:ctc)
     gene            6697..7047
                     /locus_tag="VFB37_08845"
     CDS             6697..7047
                     /locus_tag="VFB37_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HZO22599.1"
                     /translation="MYAGSAKLRRLRAVTFHLQSDPSRQLQYGLSAEEVAKVYPELVI
                     RDGSGKPEGVRDEELSPILLSKVQDGQRTIAAQRWELQQQERSIRAQENQLRELSRKP
                     AELSNRLGSNGSRQ"
     gene            complement(7071..8726)
                     /locus_tag="VFB37_08850"
     CDS             complement(7071..8726)
                     /locus_tag="VFB37_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZO22600.1"
                     /translation="MKFALLFGLIVAAGGSICEAEAPLQPRLVVAISVDQFSAALFSE
                     YRPLFTAGLRRLQSGVVFAGAYQSHAATETCPGHSTILTGSRPARTGIIANEWYDQSL
                     SRADKKVYCAEDPAQPGSSSTRYVVSPTLLRAQTLGDRMKAANAHSRVVAIAGKDRAA
                     IMMGGHATDQMWFWNGSAFVTLPGRNQPEPASVARVNASVASVIGKSAPAELPESCRI
                     RSIPVAVAGRTVGVPHERKAADLQGFRTSAAFDRATTELAIALIRELRLGTGEAPDLL
                     AVGLSATDYVGHTFGTQGAEMCAQLLQVDEDVGRILTALDATHQPYIVMLTADHGGAD
                     LPERDDIHGFPDAARADAALLPANMSRQLAAEFNLQPPVLLGVAPFGDIYLAQSIPAA
                     LREKVLQAAKARYLAQPQVAAVFTPAELRRVPFPTGPADEWSLAERFRASFDPERSGD
                     LIVAPKPHITALVDTSTYVATHGTPWNYDRKVPVLFYWPGMQGFEQPLPIETVDILPT
                     LAALIELPVPSIEIDGRCIELQPGVSERCPLERRQASQPDRAH"
     gene            9007..9963
                     /gene="cyoA"
                     /locus_tag="VFB37_08855"
     CDS             9007..9963
                     /gene="cyoA"
                     /locus_tag="VFB37_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HZO22601.1"
                     /translation="MSLRRKYMFIPHTNATSARLGRLTSYAVHRVALTLLLGLGGCST
                     GVLAPAGRVARAENVILLDALAIMLAIVIPTLIATLAIAWWFRASNRKARYRPNFAYS
                     GRIELIVWSIPTLVILFLGGVIWIGSHQLDPGNPLPADRKALDVQVVSLDWKWLFIYP
                     GQDVASVNRLVVPAGVPVHFSLTSASVMNVFFVPRLGSMIYTMNRMVTQLHLQTDGPG
                     EFFGESAQFSGDGFPYMTFSVSAVPEAQFTGWVAKAHGSGAVLDAAAYAELSRQSVSA
                     QPFTYRHLAFDVFEAIVSQKLPPGPGPESRSATPVASLRPTS"
     gene            9976..11988
                     /locus_tag="VFB37_08860"
     CDS             9976..11988
                     /locus_tag="VFB37_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZO22602.1"
                     /translation="MFGKLSWSAIPWDQPIPLTAAALVVLAIVVTLAWVLIKGYGSYL
                     WHEWITSVDHKRIGVMYISLALLMLFRGFIDAVMMRSQQALAFHANGYLPPEHYDQIF
                     SAHGTIVIFFAAMPFVIGLMNFVVPLQLGVRDVAFPTLNSTGFWLTATGAMLVNISLV
                     VGEFARTGWLVYPPLSELRYSPGVGVDYYLWAVQISGIGTLMTGINFVTTILKTRAPG
                     MSYTRMPMFCWTALASSLLIVAAFPVLTATLAMLILDRYVGFHFFTNEAGGNMMMFVN
                     LIWTWGHPEVYILVLPAFGVFSEVVATFSGKPLFGYRSMVIATMFICLVSFTVWMHHF
                     FTMGAGADVNAVFGIATSIIAVGTGVKIYNWLFTMYGGRVRFTTPMLWSVGFIVTFVI
                     GGMTGVLLAVPPADFQLHNSMFLVAHFHNVIIAGVLFGAFAGLTYWFPKAWGFRLHEG
                     WGKAAFWFSITGFYVTFVPLYVAGLLGMTRRLQRYDVPEWRPWVLAAGIGVAITACGL
                     ACQIIQLIVSVRRRSELRDLTGDPWDGRSLEWATASPPPVFNFAVLPDVRGEEPYWEI
                     KQRAMETQQLADEPRYEPLEVPRNSPTGFITAFFATVMGFAMIWHIWWLAILGLVAAY
                     VVFVWFAWRDVAHYEIPVEEVARLDRARRRAREEWLAAHARVGEPA"
     gene            11985..12668
                     /locus_tag="VFB37_08865"
     CDS             11985..12668
                     /locus_tag="VFB37_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome (ubi)quinol oxidase subunit III"
                     /protein_id="HZO22603.1"
                     /translation="MSATDLTERSWEDRRAYTFGGAGDEGHTTRYATGHGEGGPASKR
                     VIVGYGFWIFLLSDIIMFSAFFASYAVLASETAGGPSARQLFDKGSVAAETMCLLLSS
                     FTCGLASLAVDRRNLKWTQLALLITGLLGFAFLLLEMREFAGLVAQQAGPTRSAFLSA
                     FFTLVGCHGLHVTAGLLWLGTMMAQFFAKGFREDICHRYLCFSLFWHALDIIWVAIFS
                     LVYLVGTVL"
     gene            12665..13072
                     /gene="cyoD"
                     /locus_tag="VFB37_08870"
     CDS             12665..13072
                     /gene="cyoD"
                     /locus_tag="VFB37_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HZO22604.1"
                     /translation="MSSSPQQGDVQDRLDLAPGGDDLEPGSRSHAGIRAYLLGLALAI
                     ALTAASFWCAQTHLIYGPAVPMAIAVLAVAQMGIHLVFFLHITTAPDNTNNVLALAFG
                     VLIAFLVVFGSIWIMDHLNHNLMSVRELMQMQR"
     gene            13169..13813
                     /gene="cofC"
                     /locus_tag="VFB37_08875"
     CDS             13169..13813
                     /gene="cofC"
                     /locus_tag="VFB37_08875"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZO22605.1"
                     /translation="MSLTVLGRDSFFSPHRRTCALVPVKARALCKTRLAPVLCCAARV
                     ELVRWMLDAVLAAAAKSKSIQRVIVISPERDCVPPHIPLLADSGESLNSALSMARSTL
                     QGPDCMDVAILPADLPRITAREIDALVHAGRAGGFSIAPDAAGTGTNALYVAGCVSFN
                     FQFGRASREAHLEEARRRGLGAQLVHSPGLAFDVDGAAELEALEEWSWNTHLRA"
     gene            13789..16218
                     /gene="cofH"
                     /locus_tag="VFB37_08880"
     CDS             13789..16218
                     /gene="cofH"
                     /locus_tag="VFB37_08880"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HZO22606.1"
                     /translation="MEHTSTRLSGLLAAALQGESPEAAQALQLLPLASAAELMPAAQA
                     LTLEGFGESVTYSRKVFIPLTQLCRDVCHYCTFAKAPRKLAAPYLDAEQVLATARAGQ
                     RAGCKEALFTLGDKPELRYRSAATALAASGHSSTLEYLAEVAEQVLHETGLLPHLNPG
                     LMTAADLTRLRPVSVSMGLMLESAAQRLCERGGPHFGSPDKLPSLRLAMLRTAGELAV
                     PMTTGLLIGIGESRPERIESLLALREIQRLHGHIQELIVQNFRAKPGTRMARCPEPLL
                     EEQLWTISAARLIFGAGMSIQAPPNLQPGQLQHLVRAGVNDWGGVSPITPDYVNPEAP
                     WPHLRDLEMATQGAGRQLLQRLALVPAFATRPERWTDPAITTYVRRLTDARGFARQDA
                     WYAGAGSELPQSARRWLQGAELPSSALQAWRRGPSHISKTIAAARRGRALDESDIVRL
                     FSAEGRDLDVVIEAADQLRRDRVGDIVSYVVNRNINYTNICLYHCGFCAFSKGRGAAD
                     LRGPAYNLDLTEIATRTREAADAGATEVCLQGGIHPSFTGETYLDILRIVKEAVPSIH
                     VHAFSPLEIKHGADTLGMPVDAFLELLRKAGLSTLPGTAAEILDDEIRDIICPDKLRT
                     DEWLQVIAAAHEVGLQTTATIMFGHVEQPLHWARHLIRVRALQERTGGITEFVPLGYV
                     HMEAPLWRRGKTRSGPTFREAVLMHAVSRIVLDPLIPNIQTSWVKMGAHGAAVCLNAG
                     ANDLGGTLMNESITRAAGGVNGQQLDATQMQNLARLVGRPARQRTTLYRIPSEPQALS
                     VVSTRASAMPA"
     gene            16233..17240
                     /gene="fgd"
                     /locus_tag="VFB37_08885"
     CDS             16233..17240
                     /gene="fgd"
                     /locus_tag="VFB37_08885"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HZO22607.1"
                     /translation="MTIRLGYKASAEQFGPAELLDYSILAEQRGFDSVFVSDHFQPWR
                     HSGGHAPFSLAWMGALGARTSRVLIGTSVLTPTFRYHPAVIAQAFATLGVLFPDRLIL
                     GVGTGEALNEVPATGLIWPEPKERTARLKEAVALIRQLWSESRVSFEGTFFRTRNATI
                     YDKPERPVPIYVAAAGPLMAKYAGQAGDGFICTSGKPWTLYSETLLPNLEAGVRTSGR
                     AAGSFERMIELKVSYDTDRQRALEDTRHWAALALSQEERNPLEDPLEMERVADALPAE
                     RTAQRWIVSSDPDEHLSKLEPYIELGFRHLVFHAPGTDQRRFLDLYSQHVLPRLRNKY
                     G"
     gene            17233..18024
                     /gene="cofE"
                     /locus_tag="VFB37_08890"
     CDS             17233..18024
                     /gene="cofE"
                     /locus_tag="VFB37_08890"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZO22608.1"
                     /translation="MADRAAASPARELGLFALPGFPLVGRGDDLAGLTAQGLDEAGLR
                     LRPDDVLVFAQKVVSKAEGREVDLKSVTPGSRATELAGIVQKDPRLVELVLRESRRVV
                     RASKDVLIVEHRLGFVMANAGIDQSNVRAEHSADYALLLPEDPDASAQRLRQRLASLT
                     GCAPGVLINDSFGRPWRLGTVGVAIGCAGLPAVLDLRGQADLLGRPLRVTVVGHADEI
                     ASAASLLMGQAAEARPVVLVRGLTANAAAQPALALIRPSQEDLFR"
     gene            18021..18971
                     /gene="cofD"
                     /locus_tag="VFB37_08895"
     CDS             18021..18971
                     /gene="cofD"
                     /locus_tag="VFB37_08895"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZO22609.1"
                     /translation="MTSHVVALCGGVGGAKLAHGLAMALPAESLTVIVNTADDFEHLG
                     LHISPDLDSVMYALAGLSDPDRGWGQRAESWNFMEMLQQLGGPGWFRLGDRDLAVHIE
                     RSRRLSEGVRLSEVTAQLCSRLGITVRVLPMSDEPVRTRVLTDEGWLEFQEYFVHRHC
                     EPPVQAFAFAGAEAAHAVPEAARALARSDLTAIVICPSNPFVSVEPILAVSDIRLALE
                     RARAPVIAVTPIVGGKALKGPAAKMLGELGLEVTPEAVARRYTGIIDAFVVDAADPLP
                     APIRGITFFSAATIMRSEPDRLRLAQAVLRSAAELVAREP"
     gene            19021..19476
                     /locus_tag="VFB37_08900"
     CDS             19021..19476
                     /locus_tag="VFB37_08900"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZO22610.1"
                     /translation="MAEPNAPQDLPSWIKDHVTRYLATNGEDGYMWDASLGGGKGMIP
                     TLLLTTTGRKTGRTLTLPLIFGRSGSDYVVVASKGGAPNHPSWYFNLEATPEVQVQVK
                     AERFKARAHTASPEERAKLWPKMVEIYAPYADYQKKTGRQIPVVVLKRL"
     gene            complement(19486..20526)
                     /locus_tag="VFB37_08905"
     CDS             complement(19486..20526)
                     /locus_tag="VFB37_08905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZO22611.1"
                     /translation="MSASESIPRGLVAFMLAHEQFLPPELIRLGAAAERAGFDLLATS
                     DHFQPWQANERHSGEAWVTLGALGQQTQRVWMGTTVTCPTLRYHPAVVAEAFATLSQL
                     YPGRIFLGLGSGEALNEQAATGRWPAWRERWDRLIEATDLIRRLWAGEPVQHQGKHYV
                     VNGRLYDPPPKPIPLLLAANGPKAMKLAGEHGDGLVTDAQTWQQHRGEWESGARSAGR
                     NPRDMPVLVETFVVVGDEADAKSAARLWNFIPKAFKGFHNIADPAEIERRAAAELPLN
                     KVYEEWTVSTDPAKHVSTLRKLFDSGATIVNIHAGQPDQQRVLDFYGKHVLPQLKGSA
                     RESIPAGETSLP"
     gene            complement(20564..21271)
                     /locus_tag="VFB37_08910"
     CDS             complement(20564..21271)
                     /locus_tag="VFB37_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO22612.1"
                     /translation="MEDDIATHALERGGRRKFRDSREEQIFRGNARLTTVAQRGKWSR
                     LFTDPAFASRAIRYALRLPVRQQTEDRRILEQLIFPRLAARADIRSILFVGCAWYTKH
                     YERKHFPRKDYWTLDAEPGARKFGGRRHIVDRLEHLQRYFPANFFDLIICNGVLGFGL
                     DSVRDCDQAFDACYRSLRKGGQLLLGWNDMRERAPVALAEVRALRHFCVSPLEPFKTW
                     RYRTNTPYRHTYDCYRK"
     gene            21474..22463
                     /locus_tag="VFB37_08915"
     CDS             21474..22463
                     /locus_tag="VFB37_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22613.1"
                     /translation="MERTGASGLSILVALWAVSAPVAAVDRWSYEYQGVQVTAEGSSR
                     YTVHLAHNLRRLDATLIDLIPFGLRDRQPLTRIYAVSPQLRKQLDDKETTLSSFWRTN
                     AQNYLLIYNVGQDSERYRGAYTGYVRGRLSSQGDPGYPDWYSSGMGDLLGTAMLDGDQ
                     VSIGAFIADHAYTLYREGRMPVRAVLGLHWDDPLLKSEQARRIYEAESWFLVRQIVIE
                     GEHRAEFASYLSLLHKGQSAGDAFAASFKVSYEDLDRMMLEALRARRYHELSIKVPDP
                     SGPEKPQTISAEEMERRLTDLMAARRGTSDQAKAPGLASARSDTSRAARGTAP"
     gene            complement(22939..23268)
                     /locus_tag="VFB37_08920"
     CDS             complement(22939..23268)
                     /locus_tag="VFB37_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22614.1"
                     /translation="MAGTRSKIARAAREAVQRRIVAWINGQSGTQQAICESLQMRASQ
                     LHTLVNEGAERCSLEYLLDVWERCGGTYTLVLSPEVSESTLQLNHQESHSAVGARRSA
                     AGGRTRS"
     gene            23583..24032
                     /locus_tag="VFB37_08925"
     CDS             23583..24032
                     /locus_tag="VFB37_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZO22615.1"
                     /translation="MRKQFGGFLLVSTILCGLARADDGSSWASRGQLGYAKTSGNTDT
                     STGNALFHIAHEFDPWKLLFGLEGLYGSTKGETTAQAWDTYLQAHYNITDRLYAYGGV
                     RYDDNRFSGFAYQEAVTTGVGYQLIKTDATKLSAQIGVGARRLRPRS"
     gene            24056..24358
                     /locus_tag="VFB37_08930"
     CDS             24056..24358
                     /locus_tag="VFB37_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZO22616.1"
                     /translation="MSTQQLDSTTDAVVDSAMNLEHAFNPYTKLIASLAVESGKENTM
                     TTASLSLQVKMTNLLSLAAGYQIVRNSEPPPGAGPTNSSTTLNLVYELANKNVAPE"
     gene            24629..24862
                     /locus_tag="VFB37_08935"
     CDS             24629..24862
                     /locus_tag="VFB37_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22617.1"
                     /translation="MERRSSVIKRPLQALLAAGAVIAIAAIWLAGQYVSDSDRLVVIT
                     TLTIVVIFVFGMIASAASGRRRSSSSRRGKRAI"
     gene            complement(24908..25093)
                     /locus_tag="VFB37_08940"
     CDS             complement(24908..25093)
                     /locus_tag="VFB37_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22618.1"
                     /translation="MTTADYVALLSQARLILENYLFDGEELRDDVAAICQRIDDALPP
                     EMRLRPPRMTRGIERAA"
     gene            25386..26045
                     /locus_tag="VFB37_08945"
     CDS             25386..26045
                     /locus_tag="VFB37_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZO22619.1"
                     /translation="MMNPISAAPVPATFLAASFLLAITPGPGVIYIVTRSVSQGRAAG
                     FASVAGIALGNLGNGLIASLGLATLFAVSTLAFTLVKYVGAGYLIYLGIRMMLAPVAA
                     PSVRDSDPMQSRRALSRTFGDGFLVALFNPKTALFFAAFLPQFLDPSDANMTGTLRLS
                     VIFVLMAAVTDCAYAAMAGGASRWLVSHMRVPRLGRYFSGGAFIALGLFTALTGRHAR
                     S"
     gene            complement(26192..26395)
                     /locus_tag="VFB37_08950"
     CDS             complement(26192..26395)
                     /locus_tag="VFB37_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22620.1"
                     /translation="MAKRPSNDGASWSAGELRTLKQLAKSGTPTSAVARQLGRTPAAV
                     QQKAMRTGISFRAARRAGARKKK"
     gene            complement(26644..27414)
                     /locus_tag="VFB37_08955"
     CDS             complement(26644..27414)
                     /locus_tag="VFB37_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZO22621.1"
                     /translation="MSACADKALLLHGFLDGELDAANSVACEAHVRECPECSAELERL
                     RALQDQVRTAVRLEAPEHLRRKITAALKVAQDRSAASARRSRPARRLLPWTFGGSMAA
                     VAALLAFVLLIRLPERNVAGELVASHVRSLLATHLTDVATSDRHVVKPWFAGKVDFSP
                     PVVDLAEQGFPLSGGRLDYVHGRVVAALVYRRHQHIINVFVWPAESGDPAGSGTLRRD
                     GYNLLHWRQGGLQLWAVSDVEAAELQQLRAELSAKTAE"
     gene            complement(27411..27905)
                     /locus_tag="VFB37_08960"
     CDS             complement(27411..27905)
                     /locus_tag="VFB37_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO22622.1"
                     /translation="MGHPFEDLFLPHLDAAYSLARFIVRDAAAADDIVQDSYLRAFRS
                     FDSYRGGDARSWLLAIVRNCSYDWAKSHPRTTQDVQADPDELADLAQAGPEESAARRG
                     EIERLRAMICRLPEPFQRTLILRELEELSYREIAQATGVPIGTVMSRLARARQLLAAS
                     LGAR"
BASE COUNT         4947 a         8979 c         8965 g         5192 t
ORIGIN      
        1 cgccgatgcg gcagcgcgcg caggatctcg ccctcggcga tgcgctcgag gcgtggttga
       61 aaggcgctcg cggccggggc tgagacttcc aggtcgtcga agaaaatcgt cccggcaaac
      121 tccggcccat caccgatgaa caggccagtc ttcagcccga cgaaagtgac cgtacagtcg
      181 gcacgcacgg tggcccccag tggaatgcct gtgtcgctgt tgaggccgga ggggacatcc
      241 agagcaaaga tggggcgtcc cgccgtgttg atttcctgaa tgacacgctc cagttccggg
      301 cgcgtcgagc cctgcaggcc ggtgccgagg agcgcatcga cgatgacttc accctcgtgc
      361 agcagttcgg gtgaaaacgg ccgcagctgc ccctcactcg ccttgaagtc ctcgcacgcc
      421 gtgcgggcat caccccgcaa tgcctcggtg ctgactgccg caagcacgca gacggtgagt
      481 cccgaggcct gagcgaagcg acccagaacg tagccgtcgc ctgcgttgtt acctccaccg
      541 cagacaatca cgatcctgtg agcggtgggc caacgggtcc gcagatagcg cagggccgcc
      601 tccccggcgc gcttcatcaa cgtgtagccc ggcacgccca gcgtctcgat tgcgtgcgca
      661 tcgagtgcgc gaacctgcgc cgtggaatac agggcgatcg gcagggccat ggggtgatta
      721 tactgaggca ggtgccgacc tcatgagcga gccaaggatt ctcgacctgc aagcggcaaa
      781 acgagcgctc gccgagggcg cacgggcgct gggtttcagc cggctgggcg tggcgggcgt
      841 gaccattccc gaggacgagc agcatctggt gcgctggctg gacgccggct tcgagggtga
      901 gatgggctac atgcggcggc acggcacctt gcgcagccgg ccccatgagc tctctccggg
      961 ggcggtgcgg gtcgtcagcg tccgcatgga ctactggccc ggggatgcgg ctgatgcgca
     1021 gcggacactt caggatccca cactgggcta tgtgtcgcgc tatgcgctcg ggcgtgacta
     1081 ccacaagatc atgcggcgct cactggcccg cctggccgag cagctgcgct tgcggatcgg
     1141 accgtttggg tatcgggtgt gcgttgacag cgcgccggtg ctggagaaag cgttcgcgag
     1201 aaacgccggc ctgggctgga tcggcaagca caccaacctc atcgcgcagg aggcgggctc
     1261 ctggttcttc ctcggcgaga ttctgaccga cctgccgctg ccgctggacg agccggcctc
     1321 cgctcactgc ggaagctgcg aggcctgcat tccggcctgt ccgaccggag ccatcgtcgg
     1381 cccctaccgc ctcgatgcgc gccgctgcat ctcgtatctc accatcgagc acgccagtgc
     1441 catccccgag gcgttgcgcc cagccatcgg caatcgcatc tacggctgcg atgactgtca
     1501 gctcgtctgt ccctggaaca agtttgcgcg cgacgccgcg catcctgact tcaaggtccg
     1561 ccatcgcctg gatgccactc atctgagtgc actgttcgcc tggacgccag cgcagttcga
     1621 ggagcgcatg cgcggctcgg cgatctatcg gatcggctat gagcgctggt cacgcaacat
     1681 cgcggtcgca ctcggcaacg cgccctcttc cacggaggtt ctgaccgctt tgcgctcacg
     1741 gtccaccgat tcctcggcaa tggtccgtga gcacgtggaa tgggcgcttg cccggcatcg
     1801 gcacgagcgc gaaccccact gatcgagttc tgtcagcgct cgacctgaat gttgtcgatc
     1861 aggcgtgcct tgcccagatg tgccgccgtg agcacgacca ggtgccgggt cctgggctcg
     1921 ggtgcggcca ggttagcggc ctggcggatc gcgaaatagt caggccggaa cccggcgccc
     1981 tcgagcgttt gcaccccgaa tcgctccatg ctggcgaact cgttatcgcc ggcctgaagt
     2041 cgcgcggcca ctgcgctgag ggttgcatgg atgcgcggtg cgagctgacg ctgctgctcg
     2101 gtcagatatt ggttgcgcga gctcatcgca agcccgtcgg cgtcccggac ggtgggcgcg
     2161 cccacgatcc ggacgggaaa gcacagatcg gcgaccatcc gccggatgat cgtgagctgc
     2221 tggaaatcct tctcgccgaa caccgcaaca tccggctgca cgatatgaaa gagtgccgcg
     2281 acgacggtgg tcacgccttc gaagtgtcct ggccggaact caccgcacag gatgcgcgac
     2341 agtgccggga cttcgacccg ggtcgcgcgc tcagcgccgc tcggatagat ctcggtgaca
     2401 tccggcatga acatcagatc gcatccggca tcggcaagca tccgctcgtc atcgcgcggc
     2461 gtgcgcggat aatgcgcgaa gtcctcgttc ggaccgaact gcatcgggtt gacgaaaata
     2521 ctggcgacga aacgatcgcc aacctgacgc gcggcctcga tcagactcac gtgccccggg
     2581 tgcaggttac ccatggtcgg aacgaaggcc acgcgcaggc cggcggcgtg ccaggcacgc
     2641 acgcgctcgc ggacagtcgc cacgctcgtc actgtttcca tgctgcggga tcagctcaaa
     2701 agcaatgctc gggagcggga tactcgcctg ttctgacggc ctgcacgtag ttggcgagcg
     2761 cctgttgatt ggtattcacg ccttccatga aattgcgcac gaagcgcggc ttgcgtcccg
     2821 tcgtgatgtc caggatgtcg tagatgacca gaatctgccc gtcggtgtcg ggacctgcac
     2881 cgattccgat gactggcacg tgcagcgcct cggtgacagc acgaccgagc gctgcgggga
     2941 tgcattcgag caagacgatg tccgcccccg cagattccaa ccgcttcgcg ttctcgatca
     3001 tccgctctgc ggcatcgtcg ctacgcccct gcacgcggaa tccgcctgtc ttgtgcacgg
     3061 actggggctt gagcccgaga tgggcgcaca cggcgatgtc atgtgcagcg agaaattcga
     3121 cgatttcgat ctgcagcgcg ccgctttcca gcttgaccat cttggccccg ccctcctgca
     3181 tgaggcgcac ggcgttggcg agggcttgct cgcgcgatgg atagctcatg aacggaagat
     3241 cgccgatgag gaacggccga tgcagtcccc gcgccaccgc acggcagtga tagacgatgt
     3301 ggtccatggt gaccggcacc gtggtgtcat aaccctggat gaccatgccg agcgagtcgc
     3361 ccaccagcac gacatccaca tccgcgttat cgagcagcac ggcgaaactg gcgtcgtagc
     3421 aggtgaggct cgcaatcttg tgccgatcgg ctttcatctg cgcgagggtg ctcagggtca
     3481 ccggaggccg atccgagccg cgcaactgca ggtgggtata catggttggc tactctactc
     3541 ccctcggtac ctgggctgtc gcgcaggcga cttttccaat gactagaggc cccggctgcg
     3601 cggcggattg aagtacagcc ggccgcgctt cattcgcttg atgacgccca gcagctcgtc
     3661 atagtcggcc tggttattga tggggtcgat ggccgccgca ttcacgatca gcaagggcgc
     3721 cgccttgaac tcgtggaaga agcgagcgta ggcctcgttg agccgctcga ggtactggcg
     3781 ctcgatgctc ttctcgtaat cgataccgcg cttgccgatc cgctcgagca acacatcgac
     3841 cggcgcctgc aggtagatga ccaggtccgg cttgggcggc tggatgtcga gcttggcgta
     3901 gacctgctcg tacaggccgt attcggcctc atcgagcgtg acccgcgcga acagccggtc
     3961 cttttccaac aggtaatcgg ccacacgcac cggcgcgaat aaatcctgct gcttcaggcc
     4021 gccgagctgc tgggcgcgct ggaacaggaa atagagttgg gccggcagcg ccccggcgcg
     4081 cggattgcgg tagaagcgct ccaggaaggg attctgcgcc gcctgctcca ggaccggctg
     4141 cgccgacaga ctcgcggcca ggcgctgggc gaggctggtc ttgccgacgc cgatgggacc
     4201 ttccacgacg atgaaccggt gctcgggatc ggctggcttc gtgctcatgc ggcgcgttga
     4261 aggagatgca ggcccgtgga gcccacacgc gctttgagct cggtcacccg gccgagcccg
     4321 ggcacgtcga ggtccggggc aatgtcggcc agaggataca gaacgaaatt gcggtccact
     4381 acacccggat ggggcacggt aagctcgggc ccactcaggc gctggcccgc aaagaccagc
     4441 aggtccagat cgatcacgcg ggggccccac ttggggtggc gctcgggccg gccggaggcg
     4501 gcttcgatgg cgcgcagctc ggcaagtagc gcatgaggct cgagctgggt cagcacgccg
     4561 gccacggcgt tcacgaaatc aggctggcgg acggctccca gaggcacgga gccatacagc
     4621 ggtgaacagc ggatgagccg ggtagcgggg atccgctcga gcgccagaca tgcccggagc
     4681 acctgcgcgc ggggatcctc cagattgctc cccactccga tgtaagcggg ccgccagaga
     4741 tccactgtcg cgcccgtcag gccggagcgg acggaccacg gcggcgccgc cggcggcgcc
     4801 caccgcgccg cggaccttcg ggccgctgcg cgtggcccgt cagggcgtcg gccatacggc
     4861 cgcgctcctc cactgaagcc tcctgaaccc gtgtccacca ttgagcaatg tccgcagagg
     4921 ccatcccgtg ctgagcgcga agcagcagca ggtcatacgc tgcgcgaaag cgcggatgct
     4981 cgagcacgcg cagcgcacga cggccgcgtg gatgctccag gcgcggctgc agcgcgaaca
     5041 tctcgcgcat gccgagcgaa aagcgcttcg gcagggagat gtgtgactgc acctcgcgag
     5101 ttgcgcgatc gcaggcatcg agaatggtcg ccagctcatg ccatcgctcg gcgggcgcag
     5161 actcgatgat gccggcaatc ggaccgtaga gcagcagcgc gaagagaaaa gtgggagtga
     5221 ccggcttgtc cgcgccgact cgtgcgtccg tattctccag gccctgaacc aacagcttct
     5281 cgaccacgct gtccggatgc gtgttgagat acttgtccac cgtcggcaac agggaggcga
     5341 gcaggccccg gtggcgcaga acctcgaaac tgcgtcggcc atgtcccgtc aggaacagct
     5401 tgagcgtctc atcgaagagg cgcgcaggcg gcacgccccg cagcagagtg cgcagccgcg
     5461 cgatcggctc ggcggtcgcg gcatcgatct cgaagcccag cttggcctcg aagcgcgcag
     5521 tgcgcagcat gcggacgggg tcttcgcgga agcgggtgtc gggatcgcct atgagcttga
     5581 gcttgcgcgc cgcgatgtct tcgaagccac caacatagtc ccagagcgag aaatcggcga
     5641 tgttgtagta gagcgcgttg gcagtgagat cgcgccgcca tacatccgca tcgatcgtgc
     5701 cgtaaatgtt gtcccgcagg atccggccgg tctcatcgaa gagccgctcg gtgctctcat
     5761 cgagcagacg ctcggcgcca tcggtacccg ccgtcccggc ctcggcgccg gatgaagcac
     5821 cattgtcagg ctccccggat tgcaactcct cgtccaggtc cgactcatcg agcgccgcgg
     5881 cctcgagcac cgcctcgtcc gcatcccggt cctcgccctc gggctcctcc ccaggtgctg
     5941 gcgcggtggc ggcgcggaag gtcgcgacct cgatgatctc gcgaccgaag aagacgtgcg
     6001 ccaggcggaa ccgtcggcca atgagccggc aattgcgaaa cagcttgcgg acctgctcgg
     6061 gcagcgcatc cgtcgctacg tcgaagtcct tcggctcgat gccgatgagc agatcgcgca
     6121 cgcaccctcc gaccaggaaa gccatgtagc ctgcatcgcg cagccgatac agcacacgca
     6181 gcgcgttggg cgagacatgg gcacgcgaaa tgttgtggtc ggatcgcggg atgatccggg
     6241 gggcgggcgt tggcgtcacc gtggacgcgg aaggtgaatt caatttccgg ctttgctttc
     6301 ggcttgagca atcaactcag tcccctataa taccgcgccc gggcaccccg ccccaccaca
     6361 gccgctccca tcgtctagag gcccaggaca tagccctctc aaggctagta caccggttcg
     6421 aatccggttg ggagcgccat aaaaatcagt aacttagttt cgatcggcgg tgttctgacc
     6481 gaaggaaggg ctatgaggga gggcgcggcc gttttcagcg ggtctgcatt gcttgaaccg
     6541 cgcctcggcg atgtcggcgc tctcggtaca caacgcgcga cattcgttgc cgggataggt
     6601 gttgcgcacg tgacggggga gcgcagtgta tatctcctcg gcgggccagc tcggcgagtt
     6661 ggcttcctgc gaacgttata agacagacat aaggcgatgt acgccggctc cgcaaagctg
     6721 cgccgcctgc gggcagtgac tttccatctg cagagcgatc ccagccggca gttgcagtat
     6781 ggtcttagcg cggaggaggt cgccaaggtc tatccggaac tcgtcatcag ggacggctcc
     6841 gggaagcccg agggagttcg cgacgaggag ctgagcccga tattgctcag caaagtacaa
     6901 gatgggcaaa ggacgatcgc ggcgcaaaga tgggagctgc agcagcagga gcgcagcatt
     6961 cgggcccagg agaaccaact tcgcgagctg agccggaaac cggcggagct gagtaatcgg
     7021 ctcgggagca atgggtcgcg gcagtagcag ggccaccatt ccggacaagt tcaatgggct
     7081 cgatcgggct gagatgcctg gcggcgctcc aacgggcacc tctcgctgac accgggctgg
     7141 agctcgatgc aacggccatc gatctcgatc gaaggaacgg gcagctcgat cagggcggcc
     7201 agggtgggca ggatgtcgac ggtctcgatc ggcagcggtt gctcgaaacc ctgcataccc
     7261 ggccaataga aaagaaccgg aaccttgcgg tcatagttcc agggtgtgcc gtgcgtcgcc
     7321 acgtaggtgc tcgtatcgac cagggccgtg atgtgcggtt tgggcgcgac gatgagatca
     7381 cccgatcgct ccggatcgaa actggcccgg aagcgctcgg ccagagacca ttcatctgca
     7441 gggccggttg gaaatggaac ccggcgcagt tcagctggtg tgaagaccgc agcaacctgc
     7501 ggctgcgcga gatagcgcgc cttggcggcc tgcagcactt tctcgcgtag cgctgccgga
     7561 atgctctgcg ccaggtagat atcgccaaag ggcgcgaccc cgagcagcac gggcggctga
     7621 aggttgaatt ccgcagccag ctgccggctc atgttggcgg gcagaagcgc cgcatcggcc
     7681 cgggcagcat caggaaatcc atggatgtca tcgcgctcgg gaaggtccgc gccgccgtgg
     7741 tcggcggtca gcatcacgat atagggctga tgcgtggcat cgagggccgt cagaatccgt
     7801 cccacatcct catcgacctg caacagctgg gcacacatct cggcgccttg cgtgccgaat
     7861 gtatgaccca cgtagtcggt tgcggaaagc ccgacggcca gcaggtcggg cgcctcgccg
     7921 gtccccagcc ggagttcgcg aatcagtgcg atcgcgagct cagtcgtggc tcggtcaaat
     7981 gcggctgagg tacggaaacc ctgcagatca gccgccttgc gctcgtgagg cacaccgacc
     8041 gtcctgccgg ccacggccac cggaatcgag cggatacgac acgattccgg cagctcggcg
     8101 ggcgcagact tgccgattac agaggcaacc gaggcattca ctctggcgac gctggcgggc
     8161 tcgggctgat tcctgcccgg cagcgttacg aaggcgctgc cgttccagaa ccacatctga
     8221 tccgtggcat ggccccccat catgatggcg gcccggtcct tgccggcaat cgctacgaca
     8281 cgactgtggg cattggccgc cttcatgcga tcacctaacg tctgcgccct gagcagcgta
     8341 ggcgaaacaa catagcgcgt cgaagaggag cctggctgtg ccggatcctc ggcgcagtaa
     8401 accttcttgt ccgccctgct gagggactgg tcgtaccatt cattggcgat gatgccggtg
     8461 cgggcggggc gagatccggt cagtatggtc gagtgcccgg ggcaggtctc ggtcgcagcg
     8521 tgactctggt aggcgcctgc aaatacaacg ccgctttgca ggcggcgcag gcccgccgtg
     8581 aatagcggcc ggtactcaga gaacagagcg gccgagaact ggtccacgga gatcgccacg
     8641 acgagccgcg gctgcaacgg ggcttctgct tcgcagattg atccgccggc ggcgacgatg
     8701 aggccaaaca gcaaggcgaa tttcacgtgg acccccgcaa ctgacgtttt cgcacgcctg
     8761 cgcgttcgag cacgcgacgc gcgatgccga aaaagcgccc ctggcgcact gccgccgccg
     8821 tcggccaacc aaatgctacc ccgagcacgt gtctcgccgc tcgcattggc gcatcccgac
     8881 cggaagccgc ggtttcggtc ataattgttg cgcacctcct cctgaataat cggggcaggc
     8941 catgggcatc cggcactcac gacgtccgtc atgcgctcgg aaccgtagaa cgggggccgc
     9001 gttgtgatgt cgctccggcg taagtacatg ttcatcccgc acacgaatgc cacatcggcg
     9061 cgtcttggaa ggctcacgtc gtacgcggtg catcgggttg cgctgactct cctgctcggg
     9121 cttggcggat gctccacggg tgtgctcgcc cctgccggcc gggttgcccg cgccgagaat
     9181 gtcattcttc tggatgcgct ggcgatcatg ctcgccatcg tcatcccgac tctgattgcc
     9241 acattggcca tcgcgtggtg gtttcgcgca tccaaccgca aggctcgcta ccgtccgaac
     9301 tttgcttact ccgggcgcat cgagctcatc gtatggtcga tccccaccct cgtgatcctt
     9361 tttctggggg gcgtcatctg gatcggctcc catcagctcg atccgggaaa tcccctgccc
     9421 gctgaccgta aagcccttga cgtgcaggtg gtctcgctcg actggaagtg gctcttcatt
     9481 tacccgggac aggacgtggc cagcgtaaac cgcctggtgg tcccggctgg cgtgcccgtg
     9541 cacttctcgc tcacctctgc gagcgtcatg aatgtgttct tcgttccgcg gctcggcagc
     9601 atgatctata cgatgaatcg catggtgacg cagcttcatc tgcagacgga tggtccggga
     9661 gagttcttcg gcgagtcggc gcagttcagc ggcgacggat ttccctacat gaccttcagc
     9721 gtgagcgcgg tgcctgaggc acagttcacc ggctgggttg ccaaggcgca cggttcgggt
     9781 gcggtgctcg atgcagcggc ctacgccgag ctttccaggc agagcgtcag tgcgcagcct
     9841 ttcacctatc gccacctcgc tttcgatgtc ttcgaggcga tcgtctccca gaagcttccg
     9901 cccggccccg gcccggagtc gagatccgcc accccggtgg cttcgctccg gccgacttcc
     9961 tgaaggggat cattcgtgtt cggcaaactc tcgtggagtg ccattccctg ggatcagccc
    10021 attccgctga ccgccgcagc gctcgtggtg ctggcgattg tcgtcaccct cgcctgggtt
    10081 ctcatcaagg gctacggatc ctatctgtgg cacgagtgga tcacgagcgt ggaccacaag
    10141 cgcatcggcg tcatgtacat ctcgctggcg ctgctcatgc tgtttcgcgg attcatcgac
    10201 gcggtcatga tgcgatcgca gcaggccctc gccttccatg cgaacggtta tctgccgccg
    10261 gagcattacg accagatctt ctccgcgcac ggcacgatcg tgatcttctt cgccgcgatg
    10321 cccttcgtca tcgggctgat gaacttcgtg gtgcccctgc agctcggcgt gcgcgacgtc
    10381 gccttcccta ccttgaactc gaccggtttc tggctcaccg cgacgggcgc catgctggtg
    10441 aacatctcgc tggtcgtcgg ggaattcgcc cgcaccgggt ggctggtcta tccgccgctg
    10501 tcggagctgc gctactcccc gggagtcgga gtcgattact acctgtgggc ggtgcagatt
    10561 tccggtatcg gcacgctgat gaccggcatc aacttcgtga ccacgatcct gaaaacacgc
    10621 gctccaggaa tgagctacac gcgcatgccg atgttctgct ggacggcgct cgcctcgagc
    10681 ctgctgatcg tggccgcctt tccggtgctg accgccacgc tggcaatgct gatcctcgat
    10741 cgttacgtcg ggtttcactt cttcaccaat gaagccggcg gcaacatgat gatgttcgtg
    10801 aacctcatct ggacgtgggg ccacccggag gtttacatcc tggtgctgcc tgccttcggc
    10861 gtcttctccg aggtggtcgc gacgttctcg ggcaagccgc tgttcggcta ccgctccatg
    10921 gtgatcgcga cgatgttcat ctgtctcgtc tccttcacag tgtggatgca ccacttcttc
    10981 accatgggcg ccggcgcgga cgtgaacgcc gtctttggca tcgccaccag catcatcgcc
    11041 gtgggtaccg gcgtgaagat ctacaactgg ctcttcacga tgtacggcgg ccgtgtccgt
    11101 ttcactactc ccatgctctg gtccgtcgga ttcatcgtga ccttcgtcat cggcggcatg
    11161 acaggcgtgc tgcttgccgt gccgccggcg gactttcagc tgcacaacag catgttcctg
    11221 gtcgctcact tccacaatgt gatcatcgct ggagtcctgt tcggagcctt cgcgggactg
    11281 acctactggt ttcccaaggc ctggggattt cggctgcacg agggctgggg caaggccgcc
    11341 ttctggtttt ccatcaccgg cttctatgtc accttcgtgc ccctctatgt ggccggcctg
    11401 ctcggcatga cacggcggct gcaacgttat gacgtccctg aatggcggcc atgggttctc
    11461 gcagccggta tcggcgtcgc catcactgcc tgcgggcttg cgtgtcagat cattcagctc
    11521 atcgtgagcg tgcgccgccg cagcgagcta cgcgacttga ccggtgatcc ctgggacgga
    11581 cgctcgctcg agtgggcgac cgcctcaccg ccgcctgtct ttaacttcgc ggtgctgccg
    11641 gatgtgcgcg gcgaggagcc gtattgggag atcaagcagc gcgcgatgga aactcagcag
    11701 ctcgccgatg agccccgcta cgagccgctc gaggttccgc gtaacagccc caccggattc
    11761 atcaccgcat tcttcgccac cgtaatgggg tttgcgatga tctggcacat ctggtggctg
    11821 gccatcctcg ggctcgtcgc ggcctatgtc gtcttcgtct ggttcgcctg gcgcgatgtg
    11881 gcccactatg agattcccgt ggaggaagtg gcacgactcg atcgcgcccg cagacgcgcg
    11941 cgcgaggagt ggctggcagc tcatgcgcgt gttggtgagc ccgcatgagt gccaccgatc
    12001 tcaccgagcg gagctgggag gaccgcaggg cctacacgtt cggaggcgcg ggcgatgagg
    12061 gccatacgac tcgatatgcc accggtcacg gggaaggcgg accggcgtcc aagcgtgtga
    12121 tcgtcggtta cggattctgg atcttcctgc tttctgacat catcatgttc tcggcgttct
    12181 tcgcctccta cgcggtcctc gccagcgaga cggccggcgg cccctcagcg cggcagctct
    12241 tcgataaagg cagcgtagcc gcagagacga tgtgccttct gctgtcgagc ttcacctgtg
    12301 gtctggcgag cctggccgtt gaccggcgga atctgaagtg gacgcagctt gcgctgctga
    12361 tcactggact gctgggattc gctttcctgc tgctcgaaat gcgggagttc gcaggtctcg
    12421 tggcacagca ggcaggtccg acccgaagcg ccttcctgtc ggcattcttc acgctggtgg
    12481 gctgtcacgg cttgcacgtg accgccggcc tgctctggtt gggcacgatg atggcgcagt
    12541 tcttcgccaa gggctttcgc gaggatatct gtcatcggta tctgtgcttc agcctgttct
    12601 ggcacgccct ggatatcatc tgggttgcca tcttcagcct cgtctacctg gtaggaacgg
    12661 tcctgtgagc tcaagccccc agcaaggcga tgttcaggac cggctggatc ttgcgcccgg
    12721 cggcgacgat ctcgagcctg gaagccgctc gcatgccggt attcgtgcct atctgctcgg
    12781 gcttgcgctg gccatcgcgc tcactgcagc ttcattctgg tgcgcacaga ctcatctcat
    12841 ttacggcccc gcggtgccca tggccatcgc cgtgcttgcg gtggcgcaaa tgggcatcca
    12901 cctggtcttc tttctgcaca tcacgaccgc acccgataac acgaacaacg tgctggcgct
    12961 ggccttcggt gtcctgatcg ccttcctggt cgtcttcgga tcgatctgga tcatggacca
    13021 tctcaatcac aacctgatgt cggtgcggga gctgatgcag atgcaacgct gaagcgcctg
    13081 gacctcggcg tttcagcatt agcgcggagg atcgggatga cgggcagcgt acgctgcgtt
    13141 aatgtcgagc aggattcctc ggagcgcgat gagcttgacc gtgcttggcc gcgacagttt
    13201 cttttctcct caccgccgca cctgcgcgct ggtaccggtc aaggcacgtg cgctctgcaa
    13261 gacccgtctc gctcccgtgc tctgctgcgc tgcgcgtgtt gagctcgtcc gctggatgct
    13321 cgatgcggtc ctcgcggcgg ccgcgaagtc gaagtcgatc cagcgggtga tcgtgatcag
    13381 ccccgagcgc gactgcgtgc cgccgcacat tccgctcctc gcagactcgg gtgaatccct
    13441 caacagcgct ctgtcgatgg ctcgctcgac gctgcagggg cccgattgca tggatgtcgc
    13501 gatcctgcca gcggatctgc cgaggatcac ggcccgggaa atcgatgccc tggtgcacgc
    13561 cgggcgcgcc ggcggcttct cgatcgcgcc cgatgctgcg ggcacgggga ccaatgcgtt
    13621 gtacgttgcg ggttgcgtgt ccttcaactt tcagtttggc cgggcaagcc gggaggcgca
    13681 tctcgaggaa gcacggcgca ggggactagg ggcacagctc gtgcacagtc cgggactcgc
    13741 atttgacgtg gatggcgcgg cagagctcga agctctcgag gaatggtcat ggaacacaca
    13801 tctacgcgct tgagcggatt gcttgccgcg gcgctgcagg gcgaaagtcc cgaggcggcg
    13861 caagctctgc agctcctgcc actggcctcc gcagcggagt tgatgcccgc ggcgcaagcc
    13921 ctgaccctcg agggcttcgg cgagagcgtc acgtattccc gcaaggtctt cattcccctg
    13981 actcagcttt gcagggatgt ctgccactac tgcacgtttg ccaaggcgcc gcgcaagctc
    14041 gcggcgccct atctcgatgc cgagcaggtg ctggcaacgg ctcgcgccgg gcagcgtgca
    14101 ggctgcaagg aggctctgtt cactctcggg gacaagcccg agttgcgata tcgcagcgcc
    14161 gcgactgcgc tggccgcctc gggacactcg agcacgctcg agtatctggc agaggtggcg
    14221 gaacaggttc tgcacgagac cggcctcctg ccgcacctca atcccgggct catgacggcc
    14281 gcggacctga cacggctgcg tcccgtgtcg gtctcgatgg gtctgatgct cgaaagcgct
    14341 gcgcagcgct tgtgcgagcg cggcgggccc cacttcggtt ctccggacaa gctcccttcg
    14401 ctcagactgg cgatgctacg cactgccggc gagctggcgg tgccgatgac cacggggcta
    14461 ttgatcggca tcggcgaaag ccgccctgag cggatcgagt cgctgctggc cttgcgggag
    14521 atccagcgcc tgcatggtca cattcaggag ctgatcgtgc agaacttcag ggccaagccc
    14581 ggcaccagga tggcccgctg ccccgagccg ctgctggaag agcagctctg gacgatctcc
    14641 gcagccagac tgatcttcgg cgccggcatg tcgatccagg cgcctcccaa cctgcagccc
    14701 ggccagctgc agcacctcgt tcgggcaggc gtcaatgact ggggcggcgt ttcgccgatc
    14761 acgcccgact acgtgaatcc cgaagcgccg tggccgcacc tgagggacct cgagatggcc
    14821 acgcagggtg ctggcagaca gctcctgcaa cgtcttgcgc tcgtaccggc cttcgcaacc
    14881 cgcccggagc gctggacgga tcccgccatc accacatacg tgcgccgtct caccgacgcg
    14941 cgcggcttcg cgaggcagga cgcatggtac gccggcgcag gttcggagct tccccaatcc
    15001 gcgcggcggt ggttgcaggg ggctgagctg ccctcatcgg ccctacaagc ttggcgacgc
    15061 ggcccctcac acatcagcaa gacgattgcg gccgctcggc gcggccgcgc tctcgatgaa
    15121 tcggatatcg tccgcctgtt cagcgccgaa ggccgcgatc tcgatgtggt catcgaggcc
    15181 gcggatcagt tgcgccgtga ccgggttggc gacatcgtga gctatgtcgt caatcgcaat
    15241 atcaactaca ccaacatctg cctttatcat tgcggcttct gtgccttctc gaagggccgc
    15301 ggcgcggcgg atctgcgcgg cccggcgtac aacctcgacc tgacggaaat cgccacccgc
    15361 acgcgcgagg ctgccgatgc tggcgccacg gaggtatgcc tccagggggg tatccatccg
    15421 agcttcaccg gcgagaccta cctcgacatc cttcggatcg tgaaggaagc ggtaccttcg
    15481 atacacgtgc acgcattctc gccgctggaa atcaaacacg gggccgacac attgggcatg
    15541 ccggtcgatg cgttcctgga acttctgcgc aaggccggcc tgtcgaccct tccgggtacg
    15601 gccgcggaga tcctcgatga cgagatccgg gacatcattt gccccgacaa gctgcggaca
    15661 gatgagtggt tgcaggtgat cgcagcggct cacgaagtag gcctgcagac gaccgctacg
    15721 atcatgttcg gtcacgtgga gcagccgctt cactgggcac ggcacctcat ccgggttcgt
    15781 gccctgcaag agcgcacggg tggaatcacc gagttcgtgc cgctgggcta tgtacacatg
    15841 gaagcgccgc tctggcgtcg aggcaagaca cgatccggac cgacgttccg cgaggcggta
    15901 ctcatgcatg ccgtctcgcg catcgtgctg gaccctctca tcccgaacat tcagacctcc
    15961 tgggtcaaga tgggagcgca cggcgccgcc gtatgtctca acgccggagc caacgatctg
    16021 ggaggcaccc tcatgaacga gtcgatcacg cgcgccgccg gcggagtgaa cggtcagcaa
    16081 ctcgacgcga cgcagatgca gaatcttgcc cggctcgtgg gtcgtcccgc acgccagcga
    16141 accacccttt atcgaatccc gagcgagccg caggcactct cggttgtttc gacgcgcgcg
    16201 agcgccatgc ccgcttgagc aggtaagcct ggatgacgat ccgactcgga tacaaagcct
    16261 cggccgagca gttcggacct gccgagctgc tcgactactc gattctggcc gagcagcgcg
    16321 gattcgattc cgtgttcgtg agcgaccatt ttcagccctg gcgccacagc ggcggccacg
    16381 cccccttctc actcgcgtgg atgggcgcac tcggcgcgcg cacatcacga gtgctcatcg
    16441 gcaccagcgt actgaccccg accttccgtt atcatcccgc cgtcatcgct caggcgtttg
    16501 caactctggg ggtgttgttt ccggatcgcc tgatcctcgg agtcggtacc ggtgaggctc
    16561 tcaatgaagt tccggcgacc ggcctcatct ggcccgagcc caaggagcgc accgcacgcc
    16621 tcaaggaagc agtggcgctc atccgccagc tctggagcga gtctcgggtc agcttcgaag
    16681 gcaccttctt tcgcacccgc aacgcaacca tctatgacaa gcccgagcgc ccggtgccga
    16741 tctacgtggc cgccgcggga ccgctgatgg ccaagtacgc aggacaagcc ggcgacggat
    16801 tcatctgcac cagcggcaag ccctggacgc tgtactccga gacgttattg ccaaatctcg
    16861 aagcgggcgt gcgcacctcc ggtcgcgcgg cgggctcttt cgagcggatg atcgagctga
    16921 aggtgtccta cgacaccgac cggcagcggg cgctggagga cactcggcac tgggccgcgc
    16981 tggcgctcag tcaggaagaa aggaatccac tcgaggaccc tctcgagatg gagcgggttg
    17041 cggatgcgct gccggccgag cgcactgcgc aacgctggat cgtctcctct gatcctgatg
    17101 agcacctcag taagctcgag ccttacatcg agctgggctt ccggcacctc gtctttcacg
    17161 ctccgggaac cgatcaacgc cgctttctcg atctgtactc gcagcacgtc ttgcccaggt
    17221 tgcggaacaa gtatggctga tcgcgcagcc gcgagcccgg cgcgcgagct gggtttgttc
    17281 gccctgcctg gatttccttt agtcggccgc ggggatgatc tcgcaggact gactgcgcag
    17341 ggattggacg aggccgggtt gcggctgcgg cccgatgacg tgctcgtgtt tgcgcagaag
    17401 gtcgtttcca aggctgaagg gcgcgaggta gacctcaagt ccgtcacgcc aggttcgcgt
    17461 gcaacggagc tcgccggcat cgttcagaaa gatccacgtc tggtggagct ggtgctgcgc
    17521 gagtcgcgcc gagtcgtgcg cgcgagcaag gacgtcctga tcgtcgagca ccgtttgggc
    17581 ttcgtgatgg ccaacgcggg gatcgatcaa tccaacgtgc gcgccgagca ttccgcagac
    17641 tatgcgctgc tgttgccgga agatccggat gccagtgcgc agcgactgcg acagaggctg
    17701 gccagcctca ccggttgcgc gccgggcgtg ctgatcaatg acagtttcgg gcgtccttgg
    17761 agactcggca cggtgggtgt cgcgatcggt tgcgcaggac tgcccgccgt gctcgatctg
    17821 cgcggtcaag cggatctgct cggacggccc ctgcgtgtca ctgtcgttgg gcatgcggat
    17881 gagattgcct ctgccgcctc actcctgatg ggtcaagccg ccgaagctcg tccagtagtg
    17941 ctggtgcggg gtctgacggc gaatgctgcg gcacagcccg ctctcgctct catccgtcct
    18001 tcgcaggagg acctgttccg atgacctcac atgtggtggc gttgtgcggc ggcgtcggcg
    18061 gcgccaagct cgcgcatggc cttgcgatgg cgctcccagc cgagagcctc accgtcatcg
    18121 tcaacaccgc tgatgacttc gagcatcttg gcctgcacat ctcgcccgat ctggactcgg
    18181 tgatgtacgc gctcgccggg ctctctgatc ctgatcgggg ctggggacag cgcgccgaga
    18241 gttggaactt catggagatg ttgcagcagc tgggcggccc gggctggttt cgactcggtg
    18301 accgggacct cgccgtgcac atcgaaagaa gccgccggct gagcgaggga gtccgcctca
    18361 gcgaagtcac cgcacaactt tgcagccgac tgggcatcac ggtccgggtg ctccccatga
    18421 gtgatgagcc cgtgcgcacg cgcgtgctca ccgatgaggg ctggctcgag ttccaggaat
    18481 atttcgttca tcgccactgc gagccacccg tgcaggcctt tgcgttcgcg ggcgccgagg
    18541 ctgcgcatgc cgtgcccgag gcggcgcggg ccctcgcgcg ctccgacctg accgccatcg
    18601 tcatctgtcc gtcgaacccc tttgtcagcg tcgagcccat tctcgccgtg tcggatatcc
    18661 ggcttgccct cgagcgcgcg cgggcacccg tgatcgccgt cacgccgatc gtgggcggca
    18721 aggcgctgaa gggcccggca gccaagatgc tcggcgagct gggcctcgaa gtgacgccgg
    18781 aggcggtcgc gcgccgctat acgggcatca tcgatgcttt cgtggtcgat gcggccgatc
    18841 ctttgcccgc ccctattcga ggcatcacat tcttcagcgc cgcgacgatc atgcggagcg
    18901 agccggaccg attgcgactg gcccaggcgg tgctacgcag cgccgctgag ctggtcgcac
    18961 gggagccctg accggcgtac tgtagctgat ccgattgcct gtattgagac ggaggacacc
    19021 atggctgaac cgaatgcacc gcaggacctt ccgagttgga tcaaggacca cgtcacgcgt
    19081 tacctggcca cgaacggcga ggacggctac atgtgggacg ccagtctggg cgggggcaag
    19141 ggcatgatcc ccacgctgct gttgaccact accggccgca agacaggccg gacactgacg
    19201 ctgcccctta tttttggacg ctcgggatcg gactacgtcg tggttgcttc caagggcggc
    19261 gcgccgaatc acccctcctg gtatttcaac ctcgaggcca ctcccgaagt gcaggtgcag
    19321 gtgaaagccg agcgtttcaa ggctcgcgcc cacacggcga gtcctgagga gcgggcgaag
    19381 ctgtggccca agatggtgga gatctacgcg ccctacgcgg actatcagaa gaaaaccgga
    19441 cggcagattc ccgtggtggt tctgaagcgc ctctgattcg caacttcagg gtagactcgt
    19501 ctcgcctgcg ggaatggact ccctggcgct gcctttgagc tgagggagga catgcttgcc
    19561 gtagaagtcc agcaccctct gctggtccgg ctgccccgcg tggatattga cgatcgtggc
    19621 gccgctgtcg aaaagcttgc gtaatgtgct gacatgtttc gccggatcag tgctgacggt
    19681 ccactcttcg taaaccttgt tcagaggcag ctcggctgct gcgcggcgct cgatctcggc
    19741 aggatcggca atgttgtgaa agcccttgaa cgccttgggg atgaaattcc acagccttgc
    19801 cgctgacttt gcgtcagcct cgtcccccac cacgacgaag gtctcgacga gcactggcat
    19861 gtcgcgcgga ttgcggccag ccgagcgagc gccgctctcc cattcgccgc ggtgctgctg
    19921 ccaggtctga gcatcggtaa ccaggccatc accgtgctct ccggccagct tcatcgcctt
    19981 cggcccattg gcggccagca gcagcgggat gggtttgggg ggtggatcgt acagcctgcc
    20041 gttcacgaca tagtgcttgc cctggtgttg tacgggctca cccgcccaga gccggcgaat
    20101 gaggtcggtt gcttcgatca gtcggtccca gcgctcgcgc cacgcgggcc agcgacccgt
    20161 ggcggcctgc tcattcaggg cttccccgga gccgaggccg agaaagatcc ggcccggata
    20221 gagttgactc agtgtggcaa aggcctcggc gaccaccgcc ggatggtagc gcagggtggg
    20281 acaggtcacc gtcgttccca tccagacgcg ttgggtctgc tgtccgagcg ctccgagcgt
    20341 gacccatgcc tcgcctgagt ggcgctcatt ggcttgccag ggctgaaagt gatcactcgt
    20401 cgccagcaga tcgaagccgg cccgctccgc agccgcaccg aggcggatca actcgggcgg
    20461 cagaaactgc tcgtgcgcca gcatgaatgc aaccagaccc cgggggatcg actcggatgc
    20521 ggacacggga cacctccttg aggatagcgc gcgggggcgc tactcatttt ctgtagcagt
    20581 cgtatgtatg tcggtacggc gtgttcgtgc ggtaacgcca ggtcttgaac ggctcgaggg
    20641 gacttacaca gaaatgacgc agagctcgca cctctgcgag ggcaacggga gcgcgctcgc
    20701 gcatgtcgtt ccagccgagt agaagttggc cacccttgcg cagcgagcgg taacaggcat
    20761 cgaacgcctg gtcacagtcc ctcaccgaat cgagcccgaa accaagcaca ccattgcaga
    20821 tgatgaggtc aaagaagttg gccgggaaat accgctgcag atgctcgagc cggtccacga
    20881 tgtggcgtcg tccgccgaac tttcgggccc ccggttcggc atcgagggtc cagtagtcct
    20941 tacggggaaa gtgttttcgc tcgtagtgct tcgtgtacca ggcacagccg acgaacagaa
    21001 tggaacgtat gtcggcgcgc gcggccaggc ggggaaagat gagctgctcg agaatgcggc
    21061 gatcttcggt ctgctggcgc accggcagcc gcaacgcata gcggattgcg cgtgaggcga
    21121 aggctggatc ggtaaacagt cgcgaccact tgccccgctg cgccaccgta gtcaggcgcg
    21181 cattgcctcg gaatatctgc tcttcccgtg aatctcgaaa ctttcgtctg ccgccgcgct
    21241 cgagcgcgtg cgttgcgata tcgtcttcca tgtgggctcc tcatgacgaa tgccaggagg
    21301 agagggaagt tccagacgtg cccgggacgc gtgttacgcg cgtgccaagc atccgctcaa
    21361 gggcgagttg caaacggctg ggaagcaatg gccattgcgc gacccttggg ccgaggctac
    21421 gctgttggcc gctgactcgc gtacacttcg cgccaaagaa acccggggga gttatggaga
    21481 gaaccggtgc gagtgggctg agcattctgg tcgcgctttg ggcagtctca gcaccggtag
    21541 ctgccgtaga ccgttggagc tatgagtacc agggagtcca ggtcaccgcc gaaggcagtt
    21601 cgcgctacac agtccacctc gcgcacaatt tgcgacggct cgatgccaca ctcatcgact
    21661 tgatcccatt cggtctacgg gatcgccaac ccctcacccg catttatgcc gtctccccgc
    21721 agcttcgcaa gcagctcgac gacaaggaaa ccacgctgtc ctccttctgg aggacgaatg
    21781 cgcagaacta ccttctgatc tacaacgttg gtcaggacag cgagcggtac cgcggagcct
    21841 acaccggcta tgtgcgcggc agactttcca gccagggcga ccccggttac ccggattggt
    21901 attcgagcgg catgggcgac ctgctcggaa cagcaatgct ggatggcgac caggtgagta
    21961 tcggtgcatt cattgccgat cacgcctaca cgctctacag ggagggtcgc atgccggtgc
    22021 gagcagtgct cggtttgcat tgggacgatc cgcttttgaa atccgaacag gcgcggcgga
    22081 tctacgaggc tgaatcgtgg tttctcgttc gccagatcgt gatcgagggc gagcaccgcg
    22141 ccgagttcgc cagctacctg tcgctgctgc acaaggggca gagtgccggt gatgctttcg
    22201 ccgccagctt caaagtctct tatgaggatc tggataggat gatgctcgag gcactgcggg
    22261 cgcgccgtta tcacgagctg tcgatcaagg tccccgaccc cagcggtccc gaaaagccgc
    22321 agacgatcag cgccgaggag atggagaggc gtctgacgga cctgatggcg gcgcgccgcg
    22381 gcacgagcga ccaggccaag gcaccagggc tcgcctctgc gcgctcagac acctcgcgag
    22441 ctgcccgggg caccgccccg tgaagccggg gagcgcgccg agcataattg ccgagcttca
    22501 cggatcctga ggccccggga tgcgttccta ccttaccgag cgaaacagcc gggatgaggt
    22561 atggaacacc gctggggcca tcggcatgaa gtcagcgaac aggttcgcgt gcgagcccga
    22621 ggcggccaat gggttcgagg atgcatcagc aacgccagca tcagcggtgc ctttattgtc
    22681 acccgcctgt ccgccgcccc cctgtcccat ctgagcgtcc gcttctcgag atcccgcggt
    22741 agtcacactg aagaactcga aggacaggtc gtccgcagta cgcgcaaggg actggccatc
    22801 gagtggacgg agttcgcccc cacacgggtg cggacgctca tgctgattcc cgcatccggt
    22861 cgctctggcg ggtacaggaa ccggcagaag ccactccccg acgcccctag agatgaggcc
    22921 gccctcgact gagctccttt agctacgcgt ccggccgccc gcggcgctgc gccgtgcgcc
    22981 caccgcggag tgggactcct gatgatttaa ttgcagcgtg gactcgctga cctcggggct
    23041 cagcacgagc gtgtacgtgc ccccacagcg ctcccacacg tcgagcaggt actccagcga
    23101 gcaacgctcg gcgccttcgt tgaccagggt gtgcagctgt gaagccctca tctgcagact
    23161 ctcgcagatt gcctgctgag ttccgctctg cccattgatc caggcaacga tgcgacgctg
    23221 aaccgcttcc cgcgcagctc tggcgatttt cgatcgggta ccggccattt cgcactcctt
    23281 aacggaagtc gcgggcgggt ccgcactgcg tacccggcca tgcccactac cgggcacagt
    23341 attatctctg gccgctggca cccgattatc tcgccggcgg actaaaaaca gtccacttgt
    23401 gcggcaccag cgcgccggcc cgtgcgctcg cccccgggtg ccagccacga actgccgagt
    23461 ttacatgcat ctcgatcggg caacgcacga ttgccggtgg cgtgcgggga acgcgcgcag
    23521 cagcttctgt attattcacc tcatcccctg agtgatcacg agcacgagag gtgattttgc
    23581 cgatgcgaaa gcaattcgga ggctttttgc tggtttccac cattttgtgc ggccttgcgc
    23641 gggcagacga tggcagtagt tgggcatccc gagggcagct tggttacgcc aagacgagcg
    23701 gcaatacaga taccagcacg ggcaatgccc tcttccacat cgcacacgag ttcgacccct
    23761 ggaagctgct cttcggcctg gaagggctgt acggatctac caagggtgag acgaccgccc
    23821 aagcctggga cacttatctg caggcgcatt acaacatcac cgatcggctc tatgcatacg
    23881 gcggggttcg ctacgatgac aatcgattca gcggtttcgc ttatcaggaa gccgtgacca
    23941 cgggagtggg ttatcaactc atcaagacgg acgcgacgaa gctgtcggcg cagatcggcg
    24001 ttggcgcacg ccggcttcgc ccgagatcct gaccgaagat gcggtcggtg caatcgtgag
    24061 cacgcagcag ctcgattcca ccaccgatgc ggtcgtcgat agtgcaatga acctcgagca
    24121 cgctttcaat ccttacacga agctcatcgc ctcactcgcg gtggaatccg gcaaagagaa
    24181 cacgatgacg acggccagcc tctccttgca ggtgaaaatg accaacctgc tgtcgctcgc
    24241 cgcgggctat cagatcgtgc gcaacagtga gccgccgccc ggagcgggcc cgacaaacag
    24301 cagcactacg ttgaatctcg tgtacgagct ggcgaacaag aatgttgcac cggaatagcc
    24361 gtcgctcgcg ctcgagtggg ccgcgagcca atgacgcatc agcgtctgtc aagagcgagg
    24421 ctattgcgca gcagggcgcc agggccctgc cttgacaatt ctttgcgatc aggcatccat
    24481 gcgctttcgt tcaagcacgt cggcgttttt gacagcttga tgaaaggtag gctgtgcgtg
    24541 cacagtacgc actggggatt ggattgaggc aacaacaaaa agcgccgcct ccctagcggg
    24601 cacatacgaa gtgcgattgg acatgccgat ggaacgacgc agctcagtga tcaaacgtcc
    24661 tctgcaagct ctgctcgcag ccggtgcagt cattgcgatc gccgcaatct ggctcgcagg
    24721 ccagtacgtc tcggacagcg atcgcctcgt ggtcatcacg acgctgacta tcgtagtcat
    24781 cttcgttttc gggatgatcg cgagcgcagc ctcgggacgg cggcgctcga gtagctcacg
    24841 cagaggcaag cgcgccattt gaggcggcgc ggcgcatgcg agggctgacg gccgacgcgg
    24901 gcgagaccta cgcagctctc tcgataccgc gtgtcatccg tgggggccgt aagcgcatct
    24961 cgggcggcag agcatcgtca atcctctggc agatggctgc gacgtcgtca cgcagctctt
    25021 ctccatcgaa cagatagttc tcgaggatca ggcgtgcctg gctgagcagc gccacgtagt
    25081 cggctgtcgt catcagaacc cccgtagctg tatgggcatc cagctactat atgttgtgtc
    25141 cattctacgc gaggggcggt tcgaaacaag atctggtagc tcaatgcgtc gtcgcagggg
    25201 tggaggggga ttgtcatacg cgggtcgtcc cctggaagag ggttcgagcg caaggggacc
    25261 tccccgcgta tgacaatccc cctccatcgc tcatagtcat acgcgcgtcg tcccctggaa
    25321 gagggttcga gcgcaaggga cctccccgcg tatgacaatc caccgtcccg agttagtctg
    25381 tgaccatgat gaatcccatt tctgcagcgc ccgtgcccgc cacattcctg gcggcaagtt
    25441 tcctcctggc catcacgccg ggaccgggtg tcatctatat cgtgacgcgc agtgtcagtc
    25501 aggggcgcgc ggcgggattc gcatcggttg ccggcatcgc gctcggaaat ctgggcaacg
    25561 gactcatcgc ctcactcggc ctggctaccc tttttgcggt atccaccctc gcgttcacgc
    25621 tggtcaagta tgtgggtgcg ggttacctga tctatctggg tatccgcatg atgcttgcac
    25681 ccgttgcagc gccgagtgtc agggactccg atccgatgca gtcacggcgc gcactgtccc
    25741 gaacgttcgg tgacggcttc ctggtggcct tgttcaaccc caagacggcg ctatttttcg
    25801 ctgccttcct gccgcagttc ctcgacccgt ccgatgccaa catgacggga accctgaggc
    25861 taagcgtgat attcgtgctg atggcagcgg tgaccgattg cgcctacgcg gcgatggcgg
    25921 gcggtgcgag ccgctggctg gtgagtcaca tgcgagtgcc gcgcctcggc cgctacttca
    25981 gcggcggcgc gttcatcgct ctgggcttat tcaccgcctt gacaggcagg cacgcacgaa
    26041 gctgagtacg cgtgcacggc catgccctga gcaaaactcg cagtaggcat tcccgagaag
    26101 ctgctggaac ggcagcgtcc catccgttaa gtccgctgca aattcgcagg cgcacgcgcc
    26161 gaacgctgct gtccggcgcg cgccgagatt cctacttctt cttgcgggca ccggcccgcc
    26221 gagccgctct gaaggaaatg ccggttcgca tggccttctg ctgaacggcc gcaggggtcc
    26281 gcccaagctg gcgagccacg gcactcgtcg gggttccaga cttcgcgagt tgtttcaacg
    26341 ttcgcagctc acctgcagac caggaggcac catcgttcga gggacgcttc gccattattc
    26401 gatctccaat tcacccaagc agggtccatc gggataatac acggccgacg tgcgcatcgc
    26461 gcgcccaaca tggcgtattg acatgttgta atcacttgcg gggcgcccgg ggaggcgctg
    26521 cggcgctcga ggaacgcgat gagaggagtg tgaaacgcgg tgagttggaa tgtaggtagc
    26581 acgagctgcc gctgaagcca ttgcgactta agggtcagaa cagttggtta caagctcgca
    26641 gcgctattcg gccgttttcg cgctcagttc cgcacgtagc tgctgcaact ctgccgcctc
    26701 gacgtcggag acggcccaga gctgcaggcc gccctgtctc cagtgaagaa gattgtagcc
    26761 gtcgcggcgc aacgtgccgc tgccggccgg gtcgccactc tccgcgggcc agacgaatac
    26821 gttgatgatg tgttggtgac gtctgtagac gagagctgcg accacacggc catgcacgta
    26881 atcgagccgt ccgccgctca gcggaaagcc ctgttccgcg agatcgacca cgggtggtga
    26941 gaaatcgacc ttgccggcga accagggctt gaccacgtgg cggtccgaag tcgcgacatc
    27001 cgtaagatgg gtcgccagca gcgaccggac atgactcgcc accaactcgc cagccacatt
    27061 gcgctccggc agacggatga gcagcacaaa ggccagcagc gcggccactg cggccatcga
    27121 tccgccgaaa gtccacggca agagccgcct ggcaggccgt gatctgcgcg cggatgcagc
    27181 cgatctgtcc tgcgccacct tcaaggcagc ggtaattttg cgacgcagat gctcaggagc
    27241 ctccaaccga accgcggtac gaacctggtc ttgcaatgcg cgtagccgct cgagctccgc
    27301 cgaacactcc ggacactcgc gcacgtgagc ctcgcaggcg accgagttcg ctgcatcgag
    27361 ctcgccgtcc aggaagccat gcagcagcag ggccttgtcg gcacatgcgc tcatcgtgcc
    27421 cccaggcttg cggccagcag ctgtcgcgcc cgtgccaggc gcgacatgac cgtaccgatg
    27481 ggcacgcccg tggcctgcgc gatctcacga taagacaact cctccagctc acgcaggatg
    27541 agggtgcgct gaaacggctc gggtagtcgg cagatcatcg cccgcagcct ctcgatctcg
    27601 ccgcggcgtg cggcgctttc ttccgggcca gcttgcgcaa ggtctgccag ctcatcgggg
    27661 tcggcctgga catcctgtgt agtgcgcgga tgcgatttgg cccagtcgta ggagcagttg
    27721 cgcacgatgg ccagcagcca ggatctggca tcgcccccac gataggagtc gaacgaccgg
    27781 aaggcgcgca ggtaggaatc ctggacaatg tcgtctgccg cggccgcatc ccgcacgatg
    27841 aatcgcgcca ggctgtaagc cgcatcgaga tgaggcagga aaaggtcctc gaagggatgc
    27901 cccaagctaa tcccgtgttg tcatctcagt gccaatgagt aacgcggcga cttcacggaa
    27961 ggtttcacac tttgtcccat ggagcaagac tgggccgcgg agacaattat tcccgaggag
    28021 cgcagcggcc aacccgctgc tcgagccgac catcatccta gagcggagga ccctcttttg
    28081 atg
//
LOCUS       DASRHN010000230         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_569163, whole genome shotgun sequence.
ACCESSION   DASRHN010000230 DASRHN010000000
VERSION     DASRHN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569163"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFB37_08965"
     CDS             <1..1059
                     /locus_tag="VFB37_08965"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZO22623.1"
                     /translation="HGRTQCVLIGYSDGNKEGGLCASRFVTHQAQRVLTQALASAGER
                     HVIFHARGGSIARGGGRIDALVKAAPSGAVNGVLRLTEQGEAVQHSYGFRPIAMRTLE
                     RAFNALGLSTSAAQRGVLPGDDSGLVDCARTLASASRDAYRQLVWEESEFYDYFRAVT
                     PIDVIERMQIGSRPIHRADMQGLAGLLPTPWVFAWTQTRHMLPGWYGAGTGLRAAIDR
                     HGLAQVRAAYTSWFFLRNLVDDLETMLARVDLEIAFVYEALAPQQLRRFFPSIRAEYA
                     RAREHILAIKDIGELLDSESTLQRSIRLRNPYVDPMNFMQVDLLQRWRATDRTDRDLF
                     DALLASISGIAQGLQSTG"
     gene            complement(1216..1848)
                     /locus_tag="VFB37_08970"
     CDS             complement(1216..1848)
                     /locus_tag="VFB37_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO22624.1"
                     /translation="MVTDDMVALVPQLHTFARSLTRDGVRADDLVQEALMRAFNNIDR
                     FRPGTNLKAWLFTIVRNEHYSQLRRRKFEAHGVDTDLLPEPSVPPDHDGKLELRDLNR
                     ALASLSPGQRTALILVSVSGFSYEEAATICGCAVGTIKSRVARARETLMQMLEGHLPI
                     PKGAWDDEELPLLDYPARLPAPRPPTRPSSGMNRHMPSLTLSLPLAAPAI"
     gene            2161..2406
                     /locus_tag="VFB37_08975"
     CDS             2161..2406
                     /locus_tag="VFB37_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22625.1"
                     /translation="MAKQLNPTENNPVYRIRPTGERWDVLRNSSLEPLASFADKPSAL
                     AYALNLLRAQGHDSILRTRPEFLCSIISRSCKSELVG"
     gene            2553..>2826
                     /locus_tag="VFB37_08980"
     CDS             2553..>2826
                     /locus_tag="VFB37_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HZO22626.1"
                     /translation="MSPRAALIPIASLAVLAAACATQPQPTEELTRARAVVQQADKTS
                     AQRYAAADLQRAHDELGDADRANGEHKYDEARRYAESAEVDADLASA"
BASE COUNT          499 a          891 c          934 g          502 t
ORIGIN      
        1 cacgggcgaa cgcagtgtgt gttgatcgga tattcggatg gcaacaagga gggtggcctg
       61 tgtgcctctc ggttcgtaac gcatcaggca cagcgcgttc tgactcaggc gctcgcgtcc
      121 gccggcgagc gtcacgtcat ctttcacgcg cggggcggta gcatcgcgcg cggcggtggc
      181 cgaatcgatg cgctcgtcaa ggcggcgccc agcggagccg tgaacggggt cctgcggctg
      241 actgagcagg gcgaggcggt ccagcacagc tatggtttcc ggccaattgc catgcgcacc
      301 ctggagcgcg ccttcaatgc gctcgggctg agcacctcgg cggcgcagcg cggggttttg
      361 cctggcgatg actccggcct tgtcgactgc gcgcgcacgc tggcctcggc cagccgggac
      421 gcgtaccgac agctcgtctg ggaggagagc gaattttacg actacttccg tgcggtgaca
      481 ccgatcgatg tcatcgagcg catgcagatc ggatcgcgtc cgatccatcg cgcagacatg
      541 caagggctgg cgggcctgct acccactccg tgggtgtttg cctggactca gacccgtcac
      601 atgctcccag gttggtacgg cgcagggacc ggtctcaggg ccgcaatcga ccggcatggc
      661 ctcgcacagg tgcgcgccgc ctacacgagc tggttctttc tgcgtaacct cgtggatgac
      721 ctcgagacca tgctggcgcg cgtggatctc gagatcgcct tcgtctacga ggcgctggca
      781 ccacagcaat tgcggagatt cttcccgagc atccgcgccg aatacgccag ggcccgcgag
      841 catatcctgg cgatcaagga cattggcgag ttgctcgatt ccgagtcgac cttgcagcgc
      901 tcgattcgtt tgcgcaatcc atacgtggat ccgatgaatt tcatgcaggt cgatctgctg
      961 caacgctggc gggccacgga ccggaccgac cgggaccttt tcgatgctct gctggctagc
     1021 atcagtggca tcgcgcaggg gctgcagagc acgggttgag cggcgtgcag agcaagcctt
     1081 gggcgcgcgg aagtccgagc gcccaatccc cgatgcagga ctcgcggcgc ctcgcttcgc
     1141 cggtggctcc cggctgtgtg agcagccggg agccgtagcc tctttatgtc tacgccgcgc
     1201 gcgcgagtct catccttaga tcgccggggc ggccaaaggc agtgaaagtg tcagagacgg
     1261 catgtggcga ttcatgccag aggagggcct ggtcgggggc ctgggggccg gcagcctcgc
     1321 agggtagtcg agcagcggca gctcctcgtc atcccacgca cccttcggaa tcggcagatg
     1381 gccctcgagc atctgcatga gcgtctcgcg agctcgcgct acccggctct tgatcgtgcc
     1441 gacggcgcag ccgcagatcg tggccgcttc ttcataggag aagccgctca cactcaccag
     1501 aatcaacgcg gtgcgttggc cgggcgaaag tgaggccagc gcgcggttca gatcacgcag
     1561 ctcgagcttg ccatcatgat cgggcggcac gctcggctcg gggagcagat ccgtatcgac
     1621 gccgtgcgcc tcgaacttgc ggcggcggag ctgggagtag tgctcgttgc gcacgatcgt
     1681 gaagagccag gccttcaggt tggtgccagg cctgaagcga tcgatgttgt tgaacgcgcg
     1741 catgagcgct tcctgcacga gatcgtcagc acgtacgccg tctctcgtca gggagcgggc
     1801 aaacgtatgg agttggggca cgagggccac catgtcgtcg gtcaccattt gtcgttactc
     1861 tcttttgctg agctgccgaa agtacggcgc actttgccaa gcaaggcgtc ccgcctgatg
     1921 ggcaaggtac ttgctctggt gcccgtccaa agtcggtagg gtccccgcaa acgagcacga
     1981 atccgcccga gctgttgtaa gaacagtcct acgatcctcc gtgcgagtgc cctcacagcg
     2041 gttccggctg cgccgcggtg gcgcccgcgc tggcactcgg gcgtgcttct tgctcgttat
     2101 attcgtcagg gccgagcgcc caggaggccg ggccctcggg cagtgggggt gaatggaccc
     2161 atggcaaagc agctcaatcc aaccgagaac aatcccgtct acagaatccg cccgacaggc
     2221 gagcggtggg atgtgctgcg caactcttcg ctcgaaccgc tggcgagctt cgcagacaag
     2281 ccaagcgcgc tggcctacgc cctcaacctc ctgcgtgccc aagggcacga ctcgattctg
     2341 cgcacccgac cggaatttct atgcagcatc atcagccgca gttgcaaatc ggaactagtc
     2401 ggataactcc tagagctcaa tggtccgatt gtcctacggc ggaacccgcc cagagggagg
     2461 gaacggccgg cgccccgctg acattctgca cgcggcgagt gcgtgatgat tggggctgaa
     2521 gtcgcttgcc cgaagtttct ggggagaatt ccatgtcacc acgagcagca ttgatcccga
     2581 tcgcatcact ggcggtcctg gcggccgcct gtgccaccca gccgcagcca acggaggagt
     2641 tgacgcgcgc acgcgcggtc gttcagcagg cggacaagac gagtgcgcag cggtacgcgg
     2701 cagccgacct gcagcgtgcg catgacgagc tcggcgacgc cgaccgagcg aacggcgagc
     2761 acaagtatga cgaggcccgc cgctatgcgg aatctgccga agtcgacgcc gacctcgcat
     2821 cagcgc
//
LOCUS       DASRHN010000231         6357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2382774, whole genome shotgun sequence.
ACCESSION   DASRHN010000231 DASRHN010000000
VERSION     DASRHN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382774"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..1734
                     /locus_tag="VFB37_08985"
     CDS             418..1734
                     /locus_tag="VFB37_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO22627.1"
                     /translation="MQKRTSIAYGLVAIALIAAQSSLAEPANPSSHSIVLKAAHLFDS
                     VSGTLSDHGLIVISGNKIEAVGTNASAPADAQVIDLGDATLLPGFIDAHVHLSEEAGP
                     NWYLGFYEGIMRFPAEQALYGAHYAKVTLEAGITTVRDVGSSDYISLGLRNAIRAGMI
                     PGPRLQVSNYAIGATGGHADQDPYPPQRIAVAGPILGVCNGPDECRAAVRYQIKYGAD
                     VIKFMPSGGVLSLSDPVDNPQLTQEEMNAIVSEAHNWGRKVSAHCHGDRAAKMAIAAG
                     VDSIEHGTFLKDDTLQEMKKKHVFLVATLFAGAWVGEHLDTFPPPIAVKARAAAAQAQ
                     QMFQHAVKIGVPLAMGTDAGVEPHGLNAHEFELMAKNGLPPAQVLMAGTAGGAELLGV
                     SDKVGTLSAGKLADIVAVAGNPLTDIKTTEHAVFVMKDGVIYVGGH"
     gene            1837..2157
                     /locus_tag="VFB37_08990"
     CDS             1837..2157
                     /locus_tag="VFB37_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO22628.1"
                     /translation="MPHIKVMKIDRALRALANDKRLRILEWLKQPREHFPPQVDGDLV
                     HDGVCGLLIANKLKVSQPTASEHLRILVESGVLRPKRIKQWTFYKRDEARIRQLKSDI
                     AGSL"
     gene            2190..2546
                     /locus_tag="VFB37_08995"
     CDS             2190..2546
                     /locus_tag="VFB37_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZO22629.1"
                     /translation="MSRSNWFIDPAACLAMAMAIQLSGCATMATPAPRESQADHALAD
                     RIYIALNADPVYYYRHVDVRVDDGVAELSGYIWSVDALNRAKQITAGVPGVDRVVDRL
                     ELERNGNRGGGHSGTG"
     gene            2605..3552
                     /locus_tag="VFB37_09000"
     CDS             2605..3552
                     /locus_tag="VFB37_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO22630.1"
                     /translation="MPALAILLAAGFAPYVYPEYEYRVHGKETPITAKPTSPVAGRWF
                     DDYFVVEQIDPATFAVGEPRYYQGNYSYLLLGDRRAVLFDAGTGTRDIVPVVRSLTAL
                     PVTVIPSHLHFDHVGALGRLDRTALLDVPALRDRVRDAHLTLERYEFLGFVEDLPTPT
                     FRVDEWWAPDSTIDLGSRTLQLLWTPGHTPTSVSLYDAQHAQLFVGDFLYPGHLYAFL
                     PGASRSKYLSSTRRLLATVGPTTRIYTAHMAGPPAPVRAPVLELDDLRALQTTLIAIE
                     QGRLATIDPDGLESVGGYPRVFPVRGIVAFATGYPWNNR"
     gene            3560..5944
                     /locus_tag="VFB37_09005"
     CDS             3560..5944
                     /locus_tag="VFB37_09005"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZO22631.1"
                     /translation="MTVPQRLLSLTIVLAACGLAFASPSEESELQRSAAASVAHIQGE
                     REVPRLHHAVRVQRDTWGVAHIYAQDEHDLFFAQGFVVAQDRLFQMELWKRSGQGRLA
                     ELLGPSAIQRDINARRLQYRGSMADEYRSYAPDTQAILQAFTDGINAYIADVQKPGGP
                     GLPIEFRIAGFRPEPWKPSDCLNRLAAYAMMSNASRELLHAQAVALLGADRATGLFQF
                     DPPVRLEPVPGIDLRGLTPAILADIVSSDRRIPFPASSLQESNNWTLSGKLTASGLPL
                     LANDPHRVIAQPSLRYIVHLNAPGWNVIGAGEPGLPGVAAGHNEQIAWGFTIFGLDQE
                     DLYLETLDPQDATRYKTARGWKRMRQEHETIHVRGAPDFDAVVQWTDHGPVLWADGRR
                     ALALRWIGAEPGTAGYLGSLTLDRAQNWQEFESAMPHWKVPSENIVYADRAGNIGEHS
                     TGLAPLRRNFTGLLPVPADGRFEWAGFVPNSKLPHSYNPAAGFIASANHKMIPKGYPY
                     AVGFEWAPPTRYERIREVLESASQKGRKLTMQDMEALQTDVVSLPARRLQALLRQTAD
                     SSGDAQAREARKLLLDWDCALQADSAPAALYEMWGEQLRPAVTRRAVPPAALEFVQTW
                     PLARVVDELTVPREAVFGIDAAATRDALLQQTLQAAYGELAARLGSDPGTWTWGALHK
                     AYFRHALDAGPGLSGLLDRGPVERPGDGDVVQATSFDQSSFDQIAGASYREIFDLSDW
                     DNSVAVNVPGQSGQPGSKHFDDLLPLWSAGGYFPLKYSKAAVDAVTTDTLTLRP"
     gene            complement(5950..>6357)
                     /locus_tag="VFB37_09010"
     CDS             complement(5950..>6357)
                     /locus_tag="VFB37_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO22632.1"
                     /translation="ARQLGIGLVPFSPLGRGFLTGTFQGVEQLSSDDLRRKLPRFEPA
                     HAAANQRLLVAVSAVAKRHEATPAQIALAWVLAQGKDVVPIPGTRRRTYLDQNVDALD
                     LTLTEQDMSELNTLAASVSGERYSGQMARMVER"
BASE COUNT         1150 a         2036 c         1979 g         1192 t
ORIGIN      
        1 ccagatatcc gccccggagc cactcaccgt gaaggtgcca ccgctttggg tgtacgagcc
       61 gctcgcctgc accgccccaa tgtcaccgct gctccaagta acagcgctcg cgacgctagc
      121 gaccgcgagc agcaatatcc cgacaacgag cgacccggtc ccctgcagcc gtgccatatg
      181 agttttctta tgaggtctcg atgtgtctta tcttacgcga gcttgcaatg aagcatgcaa
      241 gtggaaatgg atgagttaac agaatgcccg ggcctgttgc cggctcgtcc ggagattatt
      301 tttcttcact cgcgagttga attgacttta cgacagcaag tgaatcccct tgggcgccgg
      361 cagtgcgcta ttcttgccgg cttctcgaac cgaccgacgc ctgaaatgga ggggagcatg
      421 cagaagcgca cgagcattgc gtacggactg gttgcgatcg cgctgatcgc cgcgcaatcg
      481 agcctcgccg agccggccaa tccgagctcc cactcgatcg tcctcaaggc tgctcatctc
      541 tttgactccg tctcgggcac gctcagcgac cacgggctca tcgtgatctc gggcaacaag
      601 atcgaggcgg tggggaccaa tgcgagcgcg cccgccgatg cccaggtcat cgacctcggg
      661 gatgccaccc tgctcccagg attcatcgat gcgcacgtgc atctctccga ggaggcaggc
      721 cccaactggt acctcggctt ctatgaaggc atcatgcgtt tcccggccga gcaggccctg
      781 tacggcgcgc attacgcgaa agtcaccctc gaggcgggta ttaccacggt gcgcgatgtc
      841 ggctcatccg actacatctc tctgggtctg cgcaatgcga tccgtgccgg catgattccc
      901 ggccctcgcc tgcaggtctc caactatgcg atcggcgcga cgggcggcca tgcggatcag
      961 gatccctatc cacctcaacg cattgccgtc gcaggtccga tcctgggggt ctgcaatggc
     1021 cccgacgagt gtcgcgccgc ggtgcgctat cagatcaaat acggcgcgga tgtcatcaag
     1081 ttcatgcctt cgggtggcgt gctctcgcta tcggatcccg tggacaatcc acaattgacc
     1141 caggaggaga tgaacgcgat cgtgtcggag gcgcataact ggggccgcaa ggtgtctgcg
     1201 cactgccatg gcgaccgggc ggccaagatg gccatcgccg cgggcgtcga ctcgatcgag
     1261 catggaacct ttctcaagga cgacacgctg caggagatga agaagaagca cgtctttctc
     1321 gtggcgaccc tgtttgcggg cgcatgggtg ggcgagcacc tcgatacttt tccgccgccg
     1381 atcgccgtga aggcgcgcgc cgcggccgcc caggcccagc aaatgttcca gcacgccgtg
     1441 aagatcgggg tcccgctcgc catgggcacc gatgccggag tcgagccaca cgggttgaac
     1501 gctcacgagt tcgagctgat ggccaagaac ggcctccctc ccgctcaggt tttgatggcg
     1561 ggaacggccg gaggcgccga gttgctgggg gtctccgaca aggtcggcac gctgagtgcc
     1621 ggcaagctcg cagacatcgt cgcggtggca ggcaacccgc tcaccgacat caagaccact
     1681 gaacatgcgg tgttcgtgat gaaggatggg gtgatctacg tggggggcca ctagggcggc
     1741 agcggctgcg ccagcgctta cgtcgttcgg ccggcggctt tcgccccgca gcagcccgac
     1801 ggcgctgcgg tctgcgtcaa ttgacaacac cggatcgtgc cgcatattaa agtcatgaag
     1861 atcgaccggg ccttgcgggc gcttgctaac gacaagcgtc tgcgcatcct ggagtggctg
     1921 aaacagccac gcgagcactt tccgccccag gtggacggcg acctggtaca cgatggcgtc
     1981 tgcgggctgc tcatcgcaaa caagctcaag gtcagtcagc cgaccgcgag tgagcacctg
     2041 cgcattctcg tcgagtccgg cgtgctgcgg cccaagcgga tcaagcaatg gaccttttac
     2101 aagcgcgatg aggcgcgtat ccggcagctc aagagcgaca ttgccggttc gctctgatgc
     2161 gacttctgcc ctggcaagga ggtatccgta tgtcccgctc aaactggttt atcgacccgg
     2221 cggcctgcct ggcaatggcg atggcgatcc agctctcagg ctgcgcgacg atggccacgc
     2281 cggcgccgcg agagtcgcag gcggatcacg ccctcgcgga tcgcatctac attgctttga
     2341 atgccgaccc tgtttactac tatcggcacg ttgatgtgcg agtggacgac ggcgtcgccg
     2401 agctcagcgg ctacatctgg agcgtcgacg ccctcaacag ggcgaaacag atcactgccg
     2461 gagtacccgg cgtagaccgc gtagtggatc ggttggagct cgagcggaac ggcaaccgag
     2521 gcggcggtca ttccggaaca ggctgagcgc attcggtcag cgtcagggat ctctccgcgt
     2581 tccctgacgc tcgcagcgct tgccttgcct gcactggcta tcctgctggc agccgggttc
     2641 gcgccgtacg tgtatcccga atacgaatat cgcgtgcacg ggaaagagac tccgatcact
     2701 gcgaagccca cctctcctgt cgccgggcgc tggttcgatg actacttcgt cgttgaacag
     2761 atcgaccccg cgacctttgc ggtcggggag ccacgttact accagggcaa ctacagttac
     2821 ctgctcctcg gcgacagacg tgcggtcctg tttgatgccg gcacaggaac acgcgacatc
     2881 gtgcccgtgg tgcgctcgct gacggctctt ccggtcacgg tcattccgtc tcacctgcat
     2941 ttcgatcacg tcggcgctct tggccgcctg gatcgcacgg ccctgttgga tgtgcctgcc
     3001 ttgcgcgacc gggtgcgaga tgcgcatctg acgctcgagc ggtacgaatt cctgggcttt
     3061 gtcgaagatc tgcccacccc gaccttccgg gtggatgagt ggtgggcgcc ggatagcacg
     3121 atcgatctgg gcagtcgcac gctccagctt ctttggacgc cgggacatac tccaacgtct
     3181 gtatcgctgt acgatgcgca acacgcccag ctcttcgtgg gcgatttcct ctatcccggt
     3241 cacctgtatg ccttcctgcc gggcgcgagt cgcagcaagt acctctcgag tactcgccgt
     3301 ttgctggcca cagtcggccc gacgacccgc atctacacag cgcacatggc agggccgcca
     3361 gcgccggtga gagcaccggt gctcgagttg gatgatctga gagcgctgca gactactttg
     3421 atcgccatcg agcagggacg gctcgccact attgacccgg atggtctcga atcggtcggc
     3481 ggctatccac gggtcttccc ggtgcggggc atcgttgctt tcgccacggg ctatccttgg
     3541 aacaatcggt gaggccagaa tgacggtacc gcagcgactc ttgagtctga cgatcgtcct
     3601 cgcagcctgt gggctcgcct tcgcctcacc gtccgaggaa tccgaactgc aacgctcggc
     3661 tgccgcgagc gtcgcccaca ttcaaggcga gcgagaggtg ccccgtctac atcatgcggt
     3721 gcgcgtgcaa agggatacat ggggtgtcgc tcacatctac gcgcaagacg agcacgacct
     3781 gtttttcgcg caaggcttcg tcgttgcgca ggaccgcctc tttcagatgg agctgtggaa
     3841 acgatccggc cagggacgcc ttgcagagct cctgggcccc tcggccattc aaagggacat
     3901 caacgcacgc cgtttgcagt atcgcggcag catggccgat gaatatcgca gttatgcgcc
     3961 cgatacgcag gcgatccttc aggcgttcac cgatgggatc aacgcgtaca tcgctgatgt
     4021 ccagaaacct ggcggacccg gactaccgat cgagttcagg attgccggtt ttcgtcccga
     4081 gccgtggaag cccagcgatt gcctcaatcg gctcgccgcc tatgcaatga tgagcaatgc
     4141 ctcgcgcgag ctgctgcatg cccaggccgt agcgctgctc ggcgcggacc gggccaccgg
     4201 actgtttcag ttcgatccgc ccgtcaggct cgaaccggtg cccggcatcg atctgcgggg
     4261 gctcacacct gccattctgg cggacatcgt gagcagtgac agacgcatcc cgtttccggc
     4321 cagctcgctc caggaaagta acaactggac cctctcgggc aagctcacgg cgagcggcct
     4381 gccgctgctg gcgaacgatc cccaccgggt cattgcacaa ccctcgttgc gttacatcgt
     4441 gcatctgaat gcaccgggct ggaatgtcat cggcgccggg gagccgggat tgcccggcgt
     4501 cgccgcgggc cataacgagc agatcgcctg gggcttcacg attttcggcc tggaccagga
     4561 agacctgtat ctcgagacac tcgatccgca ggatgcgacg cgttacaaga ctgcacgtgg
     4621 ctggaagcgg atgcgtcagg agcacgagac gattcacgta cgcggcgcac ccgactttga
     4681 cgcggttgtg caatggaccg atcacggacc ggtgctgtgg gcggatggcc gtcgggcgct
     4741 ggcgctgcgc tggatcggag ctgaacccgg aaccgcaggc tatctgggct ccctgactct
     4801 cgatcgggcc caaaactggc aagagttcga aagtgcaatg ccacactgga aagtaccctc
     4861 cgagaacatc gtgtacgccg accgcgccgg gaatatcggc gagcactcga ccgggctcgc
     4921 tcccttgcgt cggaatttca ccggtttgtt gcccgtgccc gccgatggaa gattcgagtg
     4981 ggccgggttc gtgcccaaca gcaagttgcc ccacagctat aaccctgctg caggcttcat
     5041 cgcttcggcg aatcacaaga tgattccgaa aggctatccg tatgccgtgg gtttcgagtg
     5101 ggcaccgccg acccgatacg agcgcatccg cgaggtactc gagtccgcca gccagaaagg
     5161 ccgcaagctc accatgcagg acatggaggc gttgcagacc gatgtcgttt ccctccccgc
     5221 acgccgcctg caggctctgt tgcgccagac agccgattcc tcaggcgatg cacaagctcg
     5281 cgaggcgaga aagctgctcc tcgactggga ctgcgcgctg caggctgact cggcccccgc
     5341 ggcgctctac gagatgtggg gcgagcaatt gcgtccggca gtcacgcggc gcgccgtacc
     5401 gccagctgcg ctggagttcg tgcagacctg gccgctggcc cgggtagtcg atgagctcac
     5461 ggtgcctcgc gaggcggtat tcggtatcga cgccgcggca acgcgcgatg ccctgcttca
     5521 gcagacctta caggccgcct acggggagct ggcggcccga ctgggcagtg atccgggcac
     5581 atggacctgg ggagcccttc acaaagctta cttccgtcac gctctcgatg ccggccccgg
     5641 tctgtctgga ttgctcgatc gaggcccggt cgagcgcccg ggcgatgggg atgtcgtcca
     5701 ggcgacctcc ttcgatcaga gctcgttcga tcagatcgcc ggtgcgagct atcgtgagat
     5761 cttcgacctc agcgactggg acaattcggt cgccgtcaac gtacccggac agagcggcca
     5821 gcccggcagc aagcacttcg atgatctgct gccgctctgg agcgccggag gctatttccc
     5881 gctcaagtac tcgaaggctg cagtggatgc cgtcacgacc gatacgttga ctcttcgccc
     5941 ctgagcgcgt tagcgctcga ccatgcgggc catctgacct gagtagcgct cgccggaaac
     6001 actcgcggcg agcgtgttca gttctgacat gtcctgctcg gtcagagtca gatcgagcgc
     6061 atcaacgttc tgatccaggt aagtgcgccg ccgggttccc gggatcggta ccacatcttt
     6121 cccttgcgcg agcacccagg ccagtgcgat ctgcgccggt gtggcttcat gcctcttggc
     6181 aaccgcgctc acggcgacca gcagccgctg gttggccgcg gcgtgggcag gctcgaagcg
     6241 gggcagcttg cgccggaggt catcgctcga gagctgttca acaccctgga aggtcccagt
     6301 cagaaagccc cgaccgagcg ggctgaaggg gacgagtcca atgccaagct gcctcgc
//
LOCUS       DASRHN010000232        11121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1723033, whole genome shotgun sequence.
ACCESSION   DASRHN010000232 DASRHN010000000
VERSION     DASRHN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723033"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFB37_09015"
     CDS             complement(<1..775)
                     /locus_tag="VFB37_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO22633.1"
                     /translation="MQPNPMSEREQGLHRGLSKSQIVMIGLGGAIGTGLFMGSGLAIG
                     YAGPAVILSYAIAGFVALVMVFSLSEMAVVHPTAGSFGTYAETYLNPWAGFVVRYTYW
                     LAQVIAIGGEAVAAGLYMQFWFPEVPVWMWSLGFCLVLLYFNSRSVSNFGTVEFWFAL
                     IKVTAIVLFIVLGAASILGWRHAPVGLHNLTGLPGGFFPKGLSGLWMAVIVGVFSFNG
                     IEVIAVTSGEAKNPQVAIPAALRTMVLRLFLFYVLALAII"
     gene            complement(882..3242)
                     /locus_tag="VFB37_09020"
     CDS             complement(882..3242)
                     /locus_tag="VFB37_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22634.1"
                     /translation="MSIKSISRTIIPGVASLIWLSTVPRSVVAAEAAPADSTALEEVT
                     VTANKLNAQKVLDVPAAIQAISGDALQRAGAVGIMDIAGDVPGLSIQDLGPGDKKYVI
                     RGINSTGDSTAGIYYGEAVVSSTNGDDGGGFQSDIRLYDLDRVEVLRGPQGTLYGASS
                     MSGTIRFIPKLPDLHDIGGYLTLEGSETSHASGNYNFNGAVNLPIVDGLLALRLVGWK
                     LYDSGYVNQIRVGVGVTGPDGKTPVAAAGLVKGVNDDDVGGGRAMLRYQPTEDLTVDA
                     NYTSQTETSAGSSRYTPAGVAAFSGGPILPTDGCDLCNTDVTRSPWSDNLKVFGLTLT
                     YDTHFGILTGTTNQFNRTTGFTFDSTPILVSFAVPVPAETLEPRTRKVNSSEIRFASN
                     LDSPINFVAGGYREHETQNLAVEVIATNGLGFVNGPFSSANSADALNFPGVGDTFFGR
                     TDDRTDTQYAAFGEATWKITPAWTAVAGVRYFTQTLDGVQVETHPFGGFPAVPTQEAF
                     VDPTETFNKVTWRANLSYKFSEELLAYGTVSTGFRGGGLNAVSQPFEKVPAAFAPDTL
                     TNFEAGVKGRLLAGLVDYQLDAYFIRWDNMQVQQTTPDAAFVYQGNAGTAHVKGVEFE
                     FETHPIEYLTASFTGSYQDAYLVEGASPEQYGLNPTLGRTGDGIPNVPRFQADLGLNY
                     TRPIANDWQGVAAVDLDYRGRENSYFSSALLSVAPVIHENIELSPYTLVNLRLGLIKD
                     LWSVTAFARNLTDKRAQISAINSTQDPDALLTVQPRTIGLTVSRKF"
     gene            complement(3311..4369)
                     /locus_tag="VFB37_09025"
     CDS             complement(3311..4369)
                     /locus_tag="VFB37_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZO22635.1"
                     /translation="MSYPVVGRPRPHLVRVKAGRVYFWCRCGRSASQPFCDGSHRGTG
                     FSPVRFQAGPEDQDLLFCGCKETRNAPFCDGSHNNLPGGSPADDPLSAANLAIRTVER
                     QGPRALLNGGYVFSPRLAAMSSRGALRYCYLVTEQMGASHQTQILLELSDGELSPVIS
                     LGDSDVILFIAAGTGEITIAGRPFPLRADDGVYVRPGEAIQLAAQPGETLRVFMLGCP
                     IAELQWPGAMAANFDARHPQRVVSVDPAKRVAMGSRYFQLLVDKAIGSRVITQFIGHI
                     PCSKAAPHRHLYEEAIILLSGEGYMWTEDRKAPVTAGDVIFLPSRQLHSLQATSADGM
                     DVVGVICPGDNPSINYYD"
     gene            complement(4483..5160)
                     /locus_tag="VFB37_09030"
     CDS             complement(4483..5160)
                     /locus_tag="VFB37_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO22636.1"
                     /translation="MATRVTQLRILQAALELFNERGTAAVSANRIAARCRISKGNLQY
                     HFRTKREIICALFQQVVDEMDAGWYRDHLAPTLEHMATMFVRQLQLIVKYRFFYREMT
                     DLLRQDPALRRRFADNRERRLHELERFMLALQARGLMRLPADPRRLRSIIDVTWIVNE
                     NWLNYMDYHDREVTVAAILEGYSEVLEVLRPYLCADPQQITHESYLAIERLAGLSARE
                     PEELMVG"
     gene            complement(5169..6722)
                     /locus_tag="VFB37_09035"
     CDS             complement(5169..6722)
                     /locus_tag="VFB37_09035"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HZO22637.1"
                     /translation="MSERSIKLSIAALGGQGGGVLANWLIEVAEAEGYLAQSTSVPGV
                     AQRTGATIYYLEFFPRALAERAGREPVMALTPVSGDVDCVLASELAEAGRAIQRGLVT
                     PDRTILISSTHRSYVIAEKSALGSGAADTQGLLEIVRSHAKRLVLFDMEATAERNGSL
                     ISSVLLGALAGSGAMPFRRETFVAAIQRSGMAVRANLAAFEDAFQQATLGDSGYAGQG
                     RPALQGLPPQARTPALQPLLERIRRLPASAQPTILEGARRSIDYQDPAYADLYLARIE
                     RIAGCEARLHAPEVNCPLLEATARGLALWMTFEDPIRVADLKTRASRFARVRDEIRAP
                     PGQLFGITEFVRPRVAEIAGTMPAGLGRWILRSPRVSAWLARWTGGKQIRTSTVSGFA
                     LLYLLGGLKRWRRSSLRFQEENARIEDWLARVERLAQGNHSLAVEVARAQRLIKGYGE
                     THERGWRNFSILMDQLESLAVRADGAIWLGRLHEASLADEEGISLRAVLAELQNSSAG
                     QVPTQRARA"
     gene            complement(6738..8939)
                     /locus_tag="VFB37_09040"
     CDS             complement(6738..8939)
                     /locus_tag="VFB37_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZO22638.1"
                     /translation="MAERSFAREVESLRLGEGAEFRGEGILAVTKALLQSGVAYVGGY
                     QGAPISHLMDVMADAQGILSELGVHFEASASEATAAAMLAASINYPLRGAVTWKSTVG
                     TNVASDALANLASAGVKGGALIILGEDYGEGASIMQERTHAFAMKSQVWLLDPRPQLP
                     AIVRAVESGFELSEASNTPVMLMLRIRACHVYGRFVAKQNVAPPFTAAAALAAPSRDY
                     GRIILPPSTYAQEREKLEKRLPAALQFIADRKLNESFDGEVADVGIVVQGGLYNNTLR
                     ALELLGCADSFGASTLPLYVLNVTYPLVAEEWVKFCDGKRAVLIIEEGQPEFIEQQAS
                     QILRQHEIPARISGKDVLPAFGEYTVDVLRKGIGTFVDKWAPQLRSCTLGATAGVAPS
                     PNGGGSVSPPEMRRPQVSPGTSAKLAALVPPRPSGLCTGCPERPFFAAMKILQKETGP
                     LHISADIGCHSFATLPPFNMGNSIMGYGLSAAGASALGADGGKRPVSIMGDGGFWHNG
                     LTSGIGNAVFNKHDALTIIIDNGYSAATGGQDIPSSRAANRLRQGNNSIVEAVKGVGV
                     KWVRRTQTYDMRGTLKLLREALTTPLRGPKVIVAEGECQLNRQRRVRPRMRKAIESGE
                     RVVRERFGVDPDTCTGDHSCIRLSGCPSLTVKDNPDPLRADPVAHVDDSCVGCGVCGE
                     VADAAVLCPSFYRAELLYNPTRRDRWLNALRSAVIGMLQRRSERRRLRYAL"
     gene            complement(8950..9765)
                     /locus_tag="VFB37_09045"
     CDS             complement(8950..9765)
                     /locus_tag="VFB37_09045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO22639.1"
                     /translation="MSTPAAPDTRATAHQTLAQLVNGMAAGRIRVIDLTVPLEPATPT
                     IQLPPQFAPSKAFALEEISHYDERGPAWYWNNIACGEHTGTHFDAPIHWVTGKDYANN
                     ATHNIPVDRFIGPACVIDVAAQAQANPDFLLTREVVEEWERKHGRIPAHAWVLIRTDW
                     SHRKSAKEFLNAGPDGSHTPGFHPQCVPFLAKERDILGVGVETVGTDAGQAHSFDPMF
                     PCHTIMHGSNRFGLASLTNLEQLPPTGAVVIAPPLKIVNGSGSPLRVLALVPA"
     gene            9876..>11121
                     /locus_tag="VFB37_09050"
     CDS             9876..>11121
                     /locus_tag="VFB37_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZO22640.1"
                     /translation="MRRVVCLGGGPAGLYAAILLRKALPAVRVEVYERNRPDDTFGWG
                     VVFSDQTMENFRVADPQTHAAILGSFHHWDDIAIHIRGRRFLSGGHGFCGIARKRLLN
                     LLQQRAAELGVQQHFQHEVQDESAFSDADLIIAADGVNSRTRNAHAAVFQPDVDVRKC
                     RFIWLGTAQRFDAFTFAFEQTEHGWFQIHAYQFSEDLSTVIVETREETWRAHGLDRAD
                     TAQSISFCERLFGAFLDGHSLKSNAGHLRGSAWLNFNRVRCHKWHDGRIVLIGDAAHT
                     AHFSIGSGTKLAMEDAISLVGQLTAANDRDAALAAYHAERELEVLRLQSAARNRMEWF
                     ENVSRYVRLPPEQFAYSLLTGSQRIGHENLRVRDPAFVATYERWLAEQEGSGEPRPPM
                     FLPFRLRTLELVNRVVASPMAQY"
BASE COUNT         1996 a         3597 c         3598 g         1930 t
ORIGIN      
        1 cgatgatggc aagggcgagc acatagaaca gaaacagccg cagcaccatg gtgcgcagcg
       61 ccgcggggat cgccacctgc ggattcttcg cctcgcccga ggtcaccgcg atcacctcga
      121 tgccattgaa ggaaaacacc ccgacgatca cggccatcca caacccgctc agcccttttg
      181 gaaagaaccc gccgggcagg cccgtgaggt tgtgaaggcc caccggggca tgccgccagc
      241 ccaggatgct ggcggccccc agcacgatga agagcacgat cgcagtgacc ttgatgagtg
      301 caaaccagaa ctccaccgtg ccgaaattgc tcaccgagcg ggaattgaaa tacagcagca
      361 ccagacagaa gccgagcgac cacatccaga cgggaacctc cgggaaccag aactgcatgt
      421 acagcccggc ggccaccgcc tcgccgccga tcgcgatcac ctgagcgagc caataggtgt
      481 agcgcacgac gaagccagcc cacggattga gataggtctc agcgtaggtg ccaaaggatc
      541 ccgcggtcgg atgcaccacg gccatctccg acaggctgaa gaccatcacg agcgccacga
      601 atccggcgat ggcgtagctg aggatgaccg ccgggcctgc gtaaccgatc gcgagcccgc
      661 tgcccatgaa caggcccgtt ccgatggccc cgccgagccc gatcatcacg atctgggact
      721 tgctcaggcc gcgatgcaga ccctgctcgc gctcgctcat cgggttcggt tgcatgcggt
      781 tcccgtcacg tgactcgacg acggcagtgc cgccggcggt ccgaaccgga gcgtgcttca
      841 ccagcggccc gcccggcccc actcttatgg aatcaatact ctcagaactt gcgcgacacg
      901 gtaaggccga tggtgcgcgg ctgcacggtc agcagagcat caggatcctg cgtcgagttg
      961 atagccgaga tctgcgcacg cttgtcggtc aggttgcgtg cgaaggcggt cacgctccac
     1021 agatccttga tcaggcccaa tcgcaggttt accagggtgt agggggacag ctcgatgttc
     1081 tcgtgaatca cgggcgcgac cgagagcagc gcggaggaga agtaggaatt ttcgcggcct
     1141 cgatagtcga gatccaccgc tgccaccccc tgccagtcgt tcgcgatcgg ccgcgtgtag
     1201 ttcagaccca ggtcggcctg gaatcgcggc acgttcggga tcccgtcccc ggtccttccc
     1261 agggtgggat tgaggccgta ctgctcgggc gaggcaccct caacgaggta agcgtcctgg
     1321 taggagccgg tgaagctggc cgtcaggtat tcgatcggat gggtctcgaa ctcgaactcg
     1381 acgcccttca cgtgcgccgt ccccgcgttg ccctgataca cgaaggccgc atcaggggtc
     1441 gtctgctgga cctgcatgtt gtcccaacga atgaagtagg catccagctg atagtccacg
     1501 agccccgcca gcaaccggcc cttcacccct gcctcgaagt tggtcagcgt gtcaggagca
     1561 aaggccgccg gaaccttctc gaacggctga ctgaccgcat tcaggccacc gccgcggaag
     1621 ccggtcgaaa ccgttccgta ggccaacagc tcctcgctga acttgtagct gagattggcc
     1681 cgccaggtga ccttgttgaa ggtctcggtc ggatcgacga aggcctcctg ggtcggcacg
     1741 gccgggaagc cgccaaacgg gtgagtctcc acctggacgc catcgagcgt ctgggtgaaa
     1801 tagcggacac cagcgacggc ggtccacgcc ggcgtgatct tccaggtcgc ttccccgaaa
     1861 gccgcgtact gcgtgtcggt acggtcatcc gtgcggccga agaaagtgtc accgacaccg
     1921 ggaaaattca aggcgtcggc agagtttgcg ctgctgaaag gtccgttgac gaaacccagg
     1981 ccgttcgtcg cgatcacctc cacggccagg ttctgcgtct cgtgctcgcg atagccgccg
     2041 gcgacgaagt tgatgggaga atccagattg ctcgcgaagc ggatctcgct cgagttcacc
     2101 ttacgcgtgc gcggctcgag cgtctcggcg ggcactggaa ccgcgaagga caccaggata
     2161 ggagtggagt cgaacgtgaa gccggtggtg cggttgaact gattcgtggt ccccgtgagg
     2221 atgccgaagt gcgtgtcgta ggtcagggtc agtccgaata ccttgagatt gtcgctccac
     2281 ggactgcgag tcacgtcggt gttgcacaga tcacagccgt cggtaggcag aatgggaccg
     2341 ccgctgaaag ccgccactcc ggccggggtg tagcgtgagg aaccggcgga ggtctccgtc
     2401 tgggaggtgt aattggcatc gaccgtcagg tcctcggtgg gctgatagcg cagcatggcg
     2461 cggcccccgc ccacgtcatc gtcattgacg cccttcacca gtccggccgc ggcaactggt
     2521 gtcttgccat ccggcccggt cacgccgacg cccacacgga tctgattgac gtagccgctg
     2581 tcgtagagct tccacccgac cagacgcagc gccagcaggc catccacgat gggcagattg
     2641 accgcaccgt tgaagttgta gttaccgctc gcgtgtgagg tctcggagcc ctctaaggtc
     2701 agatagccgc cgatgtcgtg caggtccggc agtttcggaa tgaagcggat cgtgccgctc
     2761 atggagctcg ccccgtacag cgtcccctgc ggcccgcgca gcacctcgac acgatccagg
     2821 tcgtacagcc ggatatcgga ttggaatccg ccgccgtcgt cgccattggt ggagctgacc
     2881 accgcttcgc catagtaaat gccggcggtg gagtcgccgg tggagttgat tccacggatg
     2941 acgtacttct tgtcgcccgg accgaggtcc tggatggaca gcccgggaac gtcaccggca
     3001 atgtccatga tccccacggc ccccgctcgt tgcagggcat cgcccgaaat ggcctggatg
     3061 gcggcgggca catccagcac tttctgcgcg ttgagcttgt tggcggtgac ggtgacctcc
     3121 tcgagggcgg tgctgtctgc gggggctgcc tccgcggcga cgacgctcct cgggaccgtg
     3181 ctcagccaga tgagggatgc gacgccgggg atgatggtgc gcgatatgct cttgatagac
     3241 acgattggac ccctgttgtg gtgcgcctgg acgccggggc ctgatgcacg gccctcgagc
     3301 gatcatgcgt tcagtcgtag tagttgatac tggggttgtc gccgggacag atcacgccga
     3361 cgacatccat gccatccgcc gaagttgcct gcagcgaatg aagctgcctg ctgggcagaa
     3421 agatcacgtc cccggctgtg acaggggcct tcctgtcttc cgtccacatg tagccctcgc
     3481 ctgagaggag gatgatggcc tcctcataca gatgacggtg cggagcggcc ttggagcacg
     3541 gtatgtggcc gatgaattgc gtgatgaccc gcgagccgat cgccttgtcc acgaggagct
     3601 ggaaatagcg gctgcccatc gcgacgcgct tcgctgggtc taccgagacc acccgctgcg
     3661 ggtggcgcgc atcgaaattt gccgccatcg ctccgggcca ttgcagctcg gcaatcgggc
     3721 agcccagcat gaacacccgg agagtctcgc cgggttgggc cgccagctgg atagcctcgc
     3781 caggacggac atagaccccg tcatcagccc ggagcgggaa aggcctgccg gcgatcgtga
     3841 tctcgcctgt gccggcggcg atgaaaagta tgacgtcgct gtcgcccagc gagatcacgg
     3901 gtgaaagctc cccatcggac agctcgagca gaatctgtgt ctgatgggat gcgcccatct
     3961 gctcggtcac gagatagcaa tagcgcaggg cgccgcggct cgacatcgcg gcaagccgcg
     4021 gcgagaacac atatccgcca ttcaacaggg ccctgggccc ctgccgctcg actgttcgaa
     4081 tcgcgagatt ggcggcgctc aggggatcat cggcgggcga gccgccggga agattgttgt
     4141 gcgagccatc gcaaaagggc gcattgcggg tctctttgca gccacaaaaa agcaggtcct
     4201 ggtcctcggg accggcctgg aagcgcacgg gagaaaaacc ggtgccgcgg tgcgagccat
     4261 cacaaaaggg ctgactcgcc gagcggccgc atcggcacca gaaatagacg cggcccgcct
     4321 tgacccgcac cagatgcggt ctcggcctgc caacaaccgg gtaactcatc ggccggtact
     4381 ctcccgaagg gcgccttcct tgtacagatc aggtttaggc tgcccggcct aaactgctgc
     4441 gctctggcgc gcgccatgca tcgatgtgcg gcgccgcatc gctcagccca ccatcagttc
     4501 ctccggctcg cgggcgctga gtcccgcgag ccgctcgatc gccagatagg actcatgcgt
     4561 gatctgttgg ggatcggcac aaaggtaggg acgcaacacc tcgaggactt cggaatatcc
     4621 ctccaggatg gcggcgacgg tgacttcgcg atcgtgatag tccatgtagt tgagccagtt
     4681 ctcattgacg atccaggtca catcgatgat cgagcgaagc cgtcgcggat cggcgggcag
     4741 ccgcatcagt ccgcgtgcct gcagcgccag catgaaccgc tcgagctcgt gcagccgccg
     4801 ctcgcggttg tcagcgaagc gcctgcgcag cgccggatcc tgacgcagca ggtcagtcat
     4861 ttcccggtag aagaagcggt acttgacgat gagctgcagc tggcgcacga acatggtggc
     4921 catgtgctcg agcgtcggtg cgagatggtc ccgataccat ccggcatcca tctcatccac
     4981 gacctgctgg aacagagcgc agatgatctc gcgcttggtg cgaaagtggt actgcaggtt
     5041 tcccttgctg atgcggcagc gtgcggcgat ccgattggcc gaaacggccg cggtgccgcg
     5101 ctcattgaac agttccagcg ccgcctggag gattcgaagc tgtgtgacgc gggtggccat
     5161 gccggtccct aagctctcgc ccgttgcgtc gggacctgtc cagcggatga gttttgcagc
     5221 tctgcaagca cagctcgcaa gctgatcccc tcctcgtcgg cgagtgacgc ttcgtgcagc
     5281 cgccccagcc aaatcgcccc atccgcgcgc actgccaggc tctcgagttg gtccatgaga
     5341 atggagaagt tgcgccagcc gcgctcgtgg gtttcgccat aacccttgat caaccgctgc
     5401 gcgcgcgcta cctcgactgc cagggagtga ttgccctgcg caaggcgctc tacgcgtgcc
     5461 agccagtctt cgatacgcgc attttcctcc tggaaacgca agctcgagcg ccgccaccgc
     5521 ttcagccccc ccagcagata caacagtgca aatccgctca cggtgctggt gcggatctgc
     5581 ttcccgccgg tccagcgcgc cagccaggcg ctcacccgcg gcgagcgcag gatccaccgc
     5641 cccagcccgg cgggcatcgt tccggcgatc tcggcgaccc gcggccgcac gaactcggtg
     5701 atgccgaaca actgtccggg cggtgcgcga atctcatcgc gaacgcgtgc gaatcgggag
     5761 gcgcgtgtct tgagatccgc gacccggatc ggatcctcga acgtcatcca cagcgcaagt
     5821 cctcgagccg tggcttccag cagcggacag ttcacctccg gcgcgtgcag gcgcgcctcg
     5881 cagccagcga tgcgctcgat ccgcgcgaga tacagatctg cgtacgctgg atcctgatag
     5941 tcgatgctgc gacgtgctcc ctcgagtatg gtcggctgag cggacgctgg caggcgcctg
     6001 atccgctcga gcaggggttg cagggccggc gtgcgcgcct gcggcggcag cccctggagc
     6061 gcaggccgcc cttggcccgc atagccgcta tcgccgagcg tggcctgttg gaatgcgtct
     6121 tcgaaagcgg ccaggttggc tctgaccgcc atcccgctcc tctggatcgc agctacgaag
     6181 gtttcgcgcc gaaacggcat ggccccgctg ccggccagcg ccccgagcag taccgagctg
     6241 atgagactgc cattgcgctc cgcggtcgcc tccatgtcga acagcaccag ccttttggcg
     6301 tggctgcgca cgatctcgag cagcccttgc gtatcggccg ccccgctccc caaggcactc
     6361 ttctccgcga tgacataaga acggtgggtc gaggagatga gaatcgtgcg atcaggcgtg
     6421 accaggccgc gctggatggc gcggcccgcc tccgcgagct ccgaggcgag gacgcaatcg
     6481 acgtctccac tgaccggtgt aagagccatg accggctcgc ggccggcgcg ctcggccagc
     6541 gcgcgtggaa agaactccag gtagtagatc gtggcgccgg tgcgctgcgc gacgccgggc
     6601 accgaagtcg actgcgcaag gtagccttcg gcctcggcga cctcgatcag ccagttcgcg
     6661 agcactccgc caccctggcc accgagcgcg gctatcgaca gcttgatgga tcgctcgctc
     6721 atggcggcga ggctctctta gagggcgtag cgcaatcgcc tgcgctcgct gcgacgctgc
     6781 agcattccga tgaccgcgga tcgcagcgca tttagccacc ggtcccggcg agttgggttg
     6841 taaagcagtt ccgcccggta gaaggagggg cacagcacgg cggcgtccgc cacctcgccg
     6901 cacacaccac atccgacgca gctgtcatcg acgtgtgcaa ccggatcggc gcgcaggggg
     6961 tccggattgt ccttgacggt caaggacggg cagccggaga gccggatgca ggagtgatcg
     7021 ccggtgcagg tatcggggtc cacgccgaag cgctcgcgca ccacccgctc gccggattcg
     7081 atcgccttgc gcatgcgagg ccgcacgcgc cgctgtcggt tgagctggca ctcgccttcg
     7141 gccacgatca ccttcgggcc tcgcaacggg gtcgtcagcg cctcgcgcag gagcttcagt
     7201 gttccgcgca tgtcgtaggt ctgcgtgcgg cgcacccact tgactccaac tcccttcacg
     7261 gcctccacga tcgagttgtt accctggcgg aggcggttgg cggcgcgcga ggatgggatg
     7321 tcctgcccgc ccgtggcagc ggagtagccg ttatcgatga tgatcgtcaa agcgtcgtgt
     7381 ttgttgaaga cggcgttacc gataccgctg gtcagtccgt tatgccagaa gccgccatcg
     7441 cccatgatcg agacgggacg cttgccgccg tcggccccca gcgccgaggc gccggcggcg
     7501 ctcagaccgt agcccatgat gctgttgccc atgttgaacg gtggcagcgt ggcaaacgaa
     7561 tggcagccga tatcggcgct gatgtgcagt gggccagtct ccttctgcag gatcttcatg
     7621 gcggcaaaaa aaggccgctc aggacaaccc gtgcacagcc ccgagggtcg aggcggcacg
     7681 agcgccgcaa gtttcgcgct ggtgcctggg gagacttgag gccggcgcat ctccggcggg
     7741 ctcaccgatc cgccgccgtt cggcgagggg gccacgccag ctgtcgcacc caaggtgcag
     7801 ctgcgcagct gcggagccca cttatccacg aaggtgccga tacccttgcg cagcacatcc
     7861 accgtgtact cgccgaatgc gggcaacacg tccttacccg agatccgcgc agggatttcg
     7921 tgctggcgca ggatctgact cgcctgctgc tcgatgaact ccggctggcc ttcctcgatg
     7981 atgagcacgg cccgcttgcc gtcgcagaac ttcacccatt cctcggcaac cagcggatag
     8041 gtcacattca gcacatacag tggcagtgtg ctggcgccga acgaatctgc gcagccgagc
     8101 agctccagcg cgcgcagagt gttgttgtag aggccaccct gcacgacgat gccgacatcg
     8161 gcaacctcgc cgtcgaagct ctcattgagc ttgcgatcgg caatgaactg cagtgccgca
     8221 ggcaagcgct tctcgagctt ctcccgctcc tgtgcgtagg tcgagggtgg caggatgatg
     8281 cggccgtaat cgcgcgaggg tgctgccagt gcggccgccg cagtgaacgg cggcgctaca
     8341 ttctgctttg ccacgaagcg gccataaacg tggcaggccc ggatgcgcag catcagcatg
     8401 acgggggtgt tgctggcttc cgagagctcg aagccgctct ccaccgcgcg cacgatcgcg
     8461 gggagctgcg ggcgcggatc gagcagccag acctgcgact tcatcgcgaa ggcatgcgtg
     8521 cgctcctgca tgatgctggc gccttcgccg tagtcctcgc cgaggatgat cagtgcgccg
     8581 cctttgacac ctgccgaggc aagatttgcg agcgcatcgg aagcaacatt ggtcccgacg
     8641 gtggacttcc acgtcaccgc gccgcgcagc ggatagttga tggatgccgc gagcatcgca
     8701 gccgcggtgg cttccgaggc gctggcctcg aagtgcacac ccagctcgct cagaattccc
     8761 tgcgcatcgg ccatgacgtc catcaggtgc gagatggggg ctccctggta gccgcccacg
     8821 taggccacac ccgactgcag cagcgccttg gtcaccgcga ggatgccctc gccgcggaac
     8881 tctgcaccct cgcctagtcg cagcgactcg acttcccggg caaatgatcg ctcggccacg
     8941 ccgctgcgcc taggcaggaa ccagcgcgag cacccgcagc ggactccccg agccgttgac
     9001 gatcttcagc ggtggggcga tgaccacggc gccggtcggc ggcagctgct cgaggttggt
     9061 caggcttgcc aggccgaacc tatttgagcc gtgcatgatg gtgtggcagg ggaacatggg
     9121 gtcgaagcta tgcgcctggc ccgcatcggt gccgacggtc tcgacgccga cgccgaggat
     9181 gtcacgctcc ttggccaaaa aaggcacgca ttgtggatga aaccccggcg tgtgcgagcc
     9241 gtccggaccg gcgttcagga attccttggc gctcttgcga tgcgaccagt ccgtgcggat
     9301 gagtacccag gcgtgcgcgg ggatgcggcc atgctttcgc tcccactcct ccacgacctc
     9361 gcgggtcagc aggaagtcgg gattcgcctg cgcttgcgcg gcgacatcga tcacacaggc
     9421 gggaccgatg aaacggtcca ccggaatatt gtgcgttgcg ttgttcgcat aatccttgcc
     9481 ggtcacccaa tggatcgggg catcgaaatg cgtgccggta tgctcaccgc aggcgatgtt
     9541 gttccagtac caggcaggtc cgcgctcgtc gtaatgcgag atctcctcga gggcgaacgc
     9601 cttcgagggc gcgaactgcg gcggcagctg gatggtcggc gtggccggct cgagcggcac
     9661 ggtgaggtcg atcacacgga tgcgcccagc ggccatcccg ttgacgagct gcgcgagcgt
     9721 ctggtgggcg gtggcccggg tgtcgggcgc ggcgggtgtg ctcatgattt ctctcgtgac
     9781 gacaccgaac gaataattga cacttgaagt aactggcaat ataacatacg aggcatgctg
     9841 gcgatacggc ccatgaactc gagggagcgg ccagagtgag gcgagtcgtg tgcctgggcg
     9901 gcggcccagc cggcctctac gccgccatcc tgctccgcaa ggccttgccg gcggtaaggg
     9961 tcgaagtata cgagcgtaat cgccccgacg acacattcgg ctggggggtg gtgttctccg
    10021 atcagacgat ggagaacttc cgcgtcgccg atccgcagac gcacgccgcc atactcggca
    10081 gctttcatca ctgggatgac atcgcaatcc acatccgcgg acggcggttc ctgagcggcg
    10141 ggcatggatt ctgtggcatc gcccgcaagc gactgctcaa tctgttgcag cagcgcgccg
    10201 ccgagctggg tgtgcagcag cacttccagc acgaggttca ggatgagtcg gcattctctg
    10261 acgcggatct gatcatcgcc gcagacggcg tgaacagtcg cacgcgcaac gcccatgcgg
    10321 ccgttttcca gcccgacgtg gacgtacgca agtgccgctt catctggctc ggcacggcac
    10381 agcgcttcga tgccttcacg ttcgcattcg agcagaccga gcacggttgg ttccagatcc
    10441 acgcctacca gttcagcgag gacctctcga ctgtcatcgt cgagactcgc gaagagacct
    10501 ggcgggcgca cgggctcgat cgggccgata cggcgcagtc catctccttc tgcgagcgct
    10561 tgttcggggc gtttctggat gggcacagtc taaagagcaa cgccggccac ctgcgcggct
    10621 cggcctggct caacttcaat cgcgtgcgct gccacaagtg gcatgacggc cggatcgttc
    10681 tgatcgggga tgcggcgcat acggctcact tctcgatcgg ctcgggcacc aagctcgcca
    10741 tggaggatgc gatctcgctc gtcggtcaac tcactgcagc gaacgatcgg gatgcggctc
    10801 tggccgctta ccacgcggag cgcgagctcg aggtcctgcg cctgcagagt gcggcgcgca
    10861 acaggatgga atggttcgag aacgtctcgc gctacgtgcg cctgcctccg gagcagttcg
    10921 cgtacagtct gctcaccggc agtcagcgca tcggccacga gaacctgcgc gtgcgggatc
    10981 cagctttcgt ggccacttac gagcgctggc tcgccgagca ggaaggtagc ggggagccga
    11041 ggcctcccat gttcctgccg ttcaggctgc gcacgctgga gctcgtcaat cgcgtggtcg
    11101 cctctcccat ggcgcagtac t
//
LOCUS       DASRHN010000233         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_901669, whole genome shotgun sequence.
ACCESSION   DASRHN010000233 DASRHN010000000
VERSION     DASRHN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901669"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFB37_09055"
     CDS             complement(<1..251)
                     /locus_tag="VFB37_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22641.1"
                     /translation="MAFIPHTPEDVAAMLGAIGVSSVEQLFDEIPPALRVKSLAGVPP
                     ALNEMQIGRLMSERARADGRPLCFIGAGAYEHHIPAAVWA"
     gene            complement(251..643)
                     /gene="gcvH"
                     /locus_tag="VFB37_09060"
     CDS             complement(251..643)
                     /gene="gcvH"
                     /locus_tag="VFB37_09060"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZO22642.1"
                     /translation="MSEVPADLQYTKTHEWVRTLPDGTVEIGITDHAQHALGDLVFVE
                     VPEGGRTLAVGDPCAVVESVKAASDVYSPVAGEVTMGNPQLASQPEAVNSDPYGAGWL
                     MRMRPVKGQTSALMSAADYRELLQTEGD"
     gene            complement(643..1782)
                     /gene="gcvT"
                     /locus_tag="VFB37_09065"
     CDS             complement(643..1782)
                     /gene="gcvT"
                     /locus_tag="VFB37_09065"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZO22643.1"
                     /translation="MGRRTPLFELHQELGARIVDFGGWDMPVQYGSQIGEHHVVRRAA
                     GVFDVSHMCVVDLHGERTRAFLAALVANDVGKLKQPGKALYSCMLNESGGVIDDLIVY
                     FLAEDSFRVVVNAGTRDKDLEWTRAHAREFDVKVVERTDLAMLAVQGPDARTKAARLL
                     SSSSASAALALQPFHGAQIDEWFIARTGYTGEDGFELMLPATTAVRVWRELNALGVAS
                     CGLGARDTLRLEAGMNLYGNDMDETTHPFESGLGWTVAMEPRERRFVGRAALERVLSA
                     AASGQPGQRRLVGLILEDRGVLRSHQKVVVPGAAQGSGEITSGTFSPTLERSIALARI
                     PIAHAEQVQVDIRGKLLNARVVKPPFVRFGKPVMSNWTSKNPEKP"
     regulatory      complement(2016..2118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(2130..2223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2321..3559
                     /gene="ispG"
                     /locus_tag="VFB37_09070"
                     /gene_synonym="gcpE"
     CDS             2321..3559
                     /gene="ispG"
                     /locus_tag="VFB37_09070"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZO22644.1"
                     /translation="MSSPRRTSVQVSIGPVRVGGSAPIVVQSMTNTDTANVEATAQQV
                     KSLARAGSELVRVTVNTSEAAAAVAPIRDRLAQLGVEVPLIGDFHFNGHKLLREHPSC
                     AEALAKYRINPGNVGRGSKRDPQFVEMVETACRYGKPVRIGVNWGSLDQELLTRLMDE
                     NSASPEPQTAQLVMRRAVVLSAVENALRAEEIGLPHDRIILSAKVSGVQDLIAIYSDL
                     ADRCEYPLHLGLTEAGMGSKGIVASTAGMAILLQEGIGDTIRVSLTPEPNGDRTNEVI
                     VAQEILQTMGLRSFTPMVVACPGCGRTTSTFFQELAQRIQSHIRHRMPEWRRHYAGVE
                     DMTVAVMGCVVNGPGESKHANIGISLPGTGERPVAPVYEDGEKTVTLKGDRIAEEFQD
                     IVEQYVSRSYVRRNARSESV"
     gene            3556..>3772
                     /locus_tag="VFB37_09075"
     CDS             3556..>3772
                     /locus_tag="VFB37_09075"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZO22645.1"
                     /translation="MNNALAERKCRPCEGGIPPLARADAEKLLAGLDQGWKLGNDAHS
                     IRREFGFKNFYRTMSFVNAVAHLANIED"
BASE COUNT          714 a         1234 c         1187 g          637 t
ORIGIN      
        1 gcccagaccg ctgccgggat atgatgctcg taggcacccg cgccgatgaa gcagagaggc
       61 cgtccgtcgg cacgggcacg ctcgctcatc aaacggccga tctgcatctc gttgagcgcc
      121 ggcggcacgc ccgccagtga cttgactcgc agcgccggcg gaatctcgtc gaagagctgc
      181 tcgacgctcg acacgccgat ggcgcccagc atcgccgcga cgtcctcagg agtgtgcgga
      241 atgaaagcca tcaatcgccc tccgtctgca gcaactcgcg gtagtcggct gccgacatca
      301 gggcgctcgt ctgccccttg acgggccgca tgcgcatcag ccagcccgca ccatacgggt
      361 cggagttgac ggcttcgggc tgcgaggcga gctgtggatt gcccatcgtg acctcaccgg
      421 ccaccggaga gtagacatcg gaagcggcct tgaccgactc gacgacggca caaggatcgc
      481 cgacagccag agtgcgcccc ccttcaggca cctcgacgaa gaccaggtct ccgagcgcgt
      541 gctgggcgtg atcggtgatg ccgatctcga cggtcccatc gggcagagtg cggacccatt
      601 cgtgggtctt cgtgtactga aggtcggcgg gaacttcgct catcagggtt tctccggatt
      661 tttgctcgtc cagttggaca tcacaggctt gccgaaacgc acgaacggcg gtttgacgac
      721 gcgcgcattg agaagtttgc cgcgaatgtc gacctgcacc tgttcagcgt gggcgatggg
      781 aatgcgggca agcgcgatcg atcgctccag ggtcggtgaa aaggtgccgc tcgtgatctc
      841 tccagagccc tgtgctgcac cgggcacgac cactttctgg tgactgcgca gcaccccgcg
      901 atcctcgagt atcaggccga cgaggcggcg ctggccgggc tgacccgagg ccgcagcact
      961 cagcactctc tccagcgcag cacgcccgac gaagcgccgc tcgcgcggct ccatcgcgac
     1021 tgtccagccg agacccgact cgaacgggtg agtcgtctcg tccatgtcat tgccgtaaag
     1081 attcatgcca gcctcgagcc ggagggtgtc gcgtgcgccg aggccgcagg aagcgacacc
     1141 gagcgcattc aactcacgcc atacgcgcac tgccgtcgtg gcaggcaaca tcagctcgaa
     1201 tccgtcctca ccggtatagc cggtccgggc gatgaaccat tcatcgatct gcgcgccgtg
     1261 aaacggttgc agcgcaagcg ctgcggaagc agaggagctg gatagcagtc tggccgcctt
     1321 ggtgcgggcg tccgggccct ggacggcgag catcgcgagg tcggtgcgct ccacgacctt
     1381 gacgtcgaat tcgcgcgcat gggctcgggt ccactcgaga tccttgtctc gcgtcccggc
     1441 attcacgacc acgcgaaacg agtcttccgc caggaagtac acgatcaggt catcgatgac
     1501 gccgccggac tcattcagca tgcagctgta cagagccttg cccggctgct tcagcttccc
     1561 aacatcgttg gctaccagcg cagcgagaaa ggcgcgcgtg cgctcgccat gcaggtccac
     1621 gacgcacatg tgggagacgt caaagacgcc ggccgcgcgc cgcaccacat ggtgctcgcc
     1681 gatctgcgag ccgtactgaa cgggcatgtc ccacccgccg aagtcaacga tgcgtgcgcc
     1741 cagctcctgg tgcagttcaa ataacggcgt ccgtcgtccc atcattgatg ctccgtcgct
     1801 cattgaccga aaccggcctc atgccgtccg cagggcccca actgccccgc ccgcgcctgt
     1861 ctcctggcgg gcgaactcgc tttactgcgc ggcgacctca ccgcctccgt ctggccgccc
     1921 gcccgcgggc cgaaatcact cccctgcctg gcctgatgca aatgacaatc cggtcatttg
     1981 catccaataa aaaaggcggc aggcgcttgt gcacctgccg cccctctgtc ctgtaacctg
     2041 agagatcgga cgctccgcgg agacatcgct ccccttcggt gggtggaatt cgatccacca
     2101 ctctccagag cccgtcagga tgcgtcatcc acagcctgcc gttctgcttg cctgagcgtt
     2161 tccgggcgga acttgcgcct tcggcgttcc agactgctcc ggaatctctc cggacaggcc
     2221 tctgtgacga gcgcggcgat gataccagcc acggatcccc caggcacggc cacttggcgg
     2281 accgatgtag aataggtcca cacctccagg ccagagactt atgtcatccc cacgccgcac
     2341 ttccgttcag gtatccatcg gccccgttcg cgtaggcgga agcgcgccca tcgtcgtgca
     2401 atccatgacg aatacggaca cggcgaacgt ggaagcgacg gcgcagcagg tcaaatccct
     2461 ggcgcgagca ggctcggagc tcgtacgcgt caccgtcaac acgagcgagg ccgcagctgc
     2521 ggtggcgccc atccgcgacc ggctcgcaca actcggcgtc gaggtgcctt tgatcgggga
     2581 ctttcatttc aacggtcaca agctgctgcg tgagcaccca tcctgcgccg aagcactggc
     2641 gaaatatcgc atcaaccccg ggaacgtcgg ccgcgggagc aagcgtgacc cccagttcgt
     2701 ggagatggtc gaaacggcgt gccgctacgg gaagcccgtg cgaattggcg tgaactgggg
     2761 cagcctcgac caggagctgt tgacgcgcct gatggatgag aactccgcga gtcccgagcc
     2821 gcagactgca cagctagtga tgcgtcgggc cgtggtgctg tcggcggtcg agaatgctct
     2881 gcgcgccgag gaaatcggat tgcctcacga ccggatcatc ctgtcggcca aggtgagtgg
     2941 agtgcaggat ctgatcgcga tctacagcga cctcgccgat cgttgtgaat atcccctgca
     3001 tctgggtttg acggaagccg gaatgggctc caagggaatc gtcgcctcga cagccggcat
     3061 ggcaatcctg ctgcaggagg gcatcggcga taccatccgc gtgtcgctga cgcccgagcc
     3121 caatggcgat cgcaccaacg aggtcatcgt ggcgcaggag atactgcaga ccatgggcct
     3181 gcgctccttc acaccgatgg tggtcgcatg cccaggctgc ggacggacga ccagcacctt
     3241 cttccaggaa ctggcgcaac gcatccaatc acacatccgg catcggatgc cggagtggcg
     3301 ccggcattac gcgggagtgg aggacatgac cgtcgcggtc atgggctgcg tggtgaacgg
     3361 tccgggagag agcaaacatg ccaacatcgg catcagcctg cccgggaccg gcgagcggcc
     3421 cgtggctccc gtgtacgagg atggagagaa aacggtgacc ctgaaagggg atcgcatcgc
     3481 ggaggaattc caggacatcg tcgagcagta cgtctcccgc agctatgtgc gcaggaatgc
     3541 gcgaagtgag tccgtatgaa caatgcactg gcggaaagga aatgccggcc atgcgagggc
     3601 gggattccgc cgctggcgcg ggccgatgcc gagaagcttc tcgcggggct ggatcagggc
     3661 tggaagctcg gcaacgatgc gcactcgatc cggcgtgagt tcggcttcaa gaacttctac
     3721 cgcacgatga gcttcgtgaa tgccgtggcg cacctcgcca acatcgagga tc
//
LOCUS       DASRHN010000234        13316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1893633, whole genome shotgun sequence.
ACCESSION   DASRHN010000234 DASRHN010000000
VERSION     DASRHN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893633"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFB37_09080"
     CDS             <1..669
                     /locus_tag="VFB37_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZO22646.1"
                     /translation="YASVGYLYDYPHHVHPSEDQIKAALPAVNWRNLIFDESHHSIRF
                     GRPGMRIDLGGIGKGYAVDRGINILQARGIQHGLVTAGGDSRIIGDRMGRPWLVGIRD
                     PNDATKVVTRIPLVNSAMSTSGDYERYFDENGVRYHHIIDPHTGHSASKVRSATILGP
                     TATQTDGMSKTAFVLGPEKALEIINRMPEYDAIFVTPDGRILYSNGLRPPKPREAARA
                     HLNP"
     gene            complement(723..1106)
                     /locus_tag="VFB37_09085"
     CDS             complement(723..1106)
                     /locus_tag="VFB37_09085"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HZO22647.1"
                     /translation="MREWGLALQASLDINLEEPMATRRPTDITRKPEIPKEIPKVIKA
                     DDKAAAPSTPKAAAPSSPRVKKPAMPPASVAITEDVRRAMIAEAAYLRAERRGFAPGN
                     EREDWIAAELEVDALLKARHGGTSQ"
     gene            1358..2683
                     /locus_tag="VFB37_09090"
     CDS             1358..2683
                     /locus_tag="VFB37_09090"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZO22648.1"
                     /translation="MKIAELKRMSRPASPAVPVRGTEQQDVAIAGSAAPWHKRPAVLY
                     PALAGALVLIIVIGWLIRAWFNSGHVVSADRLQIATVSSGHFVRDVAAQGTVIAAVSP
                     TLFSIAPGTVSYLVRAGDTVTKGQIVATLDSPELNNEYQRERATLDSLNAALEHQQIE
                     IRRQLLTSKQQADLAQVAINAAERERKRADWAWGERAMSERDYRRAIDDVSTAKLNFE
                     HARDTAKLERESVVLDLRTRRLERDRQSLVVDNLARRVAELNVRSPVSGMVANLAQAE
                     KTRVAEGTPLITVVDLTAFELEFEVAEIYARDIKTGMPAEITLNGHIQPGTVTAISPE
                     VRQGQVVGRVRFSHGQPPGLRQNERAAVRIVLDERDHVLKFERGPVIDETTRYVYVVR
                     GNRAVRTPVQLGAASVSEIEALHGLAPGDRVIISDTRDYNDTPELLISN"
     gene            2694..3419
                     /locus_tag="VFB37_09095"
     CDS             2694..3419
                     /locus_tag="VFB37_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22649.1"
                     /translation="MLNMQHVGKVYRTDLIETHALRDFSLEVTAGEFVAVTGPSGSGK
                     TTFLNVAGLLETFETGTYSLDGSDVTRLSDDARSRLRNEKIGFIFQSFNLIPDLNIHD
                     NVDVPLRYRGLQASDRHERISSALELVGLASRTKHMPSQLSGGQQQRVAIARAIAGDP
                     AFLLADEPTGNLDSLMARQVMELLEQINEMGTTIVLVTHDPELARRAHRNVHVVDGQI
                     TDFRAYESRPVTASTDGAGSALG"
     gene            3437..4771
                     /locus_tag="VFB37_09100"
     CDS             3437..4771
                     /locus_tag="VFB37_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22650.1"
                     /translation="MFGYYTALAFRSFRRNPGITALMVIAVALGIAVCVMTLTVYHAM
                     SGNPIWWKNDRLYAVTMDNWDPNVPFFQDSSLPPPELTYKDAQYLFRSNIPERKVVMY
                     ATDGVISGAGSSPAEHVKTRVTTADFFAMFDVPFLYGSGWRGAADDPAQPLIVLSREE
                     NDKLFGGANSVGRVLRWNDHEFRVLGVLNDWAPRPRFYDLNGGSFGSPDAVYIPYGWG
                     AELELFNDEESDCWKSEKIDTFKDYLGSECIWQQMWVELPSRASRNRMQSFMDGYWAE
                     QRRSGRFQRPRDNRLTNVADWLRDNQVVRNDNRLLVALAFAFLAVCLINTVGLLLAKF
                     LNGASVTGVRRALGASRRQIFIQHLVEVGALSALGALLGLGFAWMGLAGVHHLYASAH
                     LGRHGGYQELTHFDSVGILWAIVLAIGATLAAGLYPAWRIGRLPPAVYLKSQ"
     gene            4807..6024
                     /locus_tag="VFB37_09105"
     CDS             4807..6024
                     /locus_tag="VFB37_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZO22651.1"
                     /translation="MSLHVRPILSALLRNRTGAVLVAMQVAITLAVLVNALYIVVQRA
                     QKIDRPTGIDLQNIFVIQSAGFTQRFQISPSLQEDLTWLRELPGVIAATPSSVIPLSN
                     GGNNEPIVTRPDLQRSEYYNEINVDEHGLDALGAHLVAGRNFRREEISPPRGKKDASR
                     FEPEIIVSRALAERLFPAQNPLGKTVYDFINQTATIIGVVDPIMGCFPSHEHPDWVFF
                     VPRLPSGEAAVNYLVRTRPGQRDSVMRLAEAHLSKSNPDRVIDSVRPLTYYRNLSYLD
                     DRSMQIYLLTVTVLLISVTCLGIFALATFNVSSRTKQIGTRRAVGARRRDIVRYFLVE
                     NALITSAGVLTGCALALGAGYWLSVEYALPRLDLYYLVGGVLVLWAIGQLAAWEPARR
                     AAAVSPSVATRTV"
     gene            6175..7575
                     /locus_tag="VFB37_09110"
     CDS             6175..7575
                     /locus_tag="VFB37_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZO22652.1"
                     /translation="MTFSNIGSVRSSLANRTVLVIDDSEAVRTAFDVLLSLHGARVLT
                     AATPADGLALLAPDAVDLVIQDMNFRREATSGEEGIALFHEIRQRSPELPIILLTAWT
                     HLETAVELVKAGAADYVAKPWDDARLLTAVRNLLDLQSARAEARAMREQRREAREALA
                     ERFDLRGIVYESEAMHTLLATATHVAQADVPILITGPNGVGKEVLADIVQANSAVRDR
                     PFLKVNLGALPNDLIEAELFGTEAGAFTGARARSGRFEAADGGTLFLDELGNLAGSGQ
                     AKLLRVLQTGEFERLGSNSTRRTRVRIIAATNSNLRQAIREGRFREDLFYRLNVIELE
                     IPPLAERREDIVALARHFLQPGYELSPEALQALCRYSWPGNVRELQNSIRRACLLCSE
                     GVLKPGDLNLPSEPLPAAAEEPALDRMAIEQALGRAQGVIAQAARDLGLSRQALYRRM
                     EKLGLKTEMARATPGS"
     gene            7686..8987
                     /locus_tag="VFB37_09115"
     CDS             7686..8987
                     /locus_tag="VFB37_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22653.1"
                     /translation="MRRSLSGRAAIVLATVAIISAAAAAVAARFFRSTLLIALIGVLV
                     ATPAILWLARRFTRPWTRILLAVRDGITSMRDHDFSVSISRISDDELGELVSAYNSLG
                     DLLRRQRLDINQRELLLDTVIQATPLVMVLTDSAGRVVYSNLAARQLLYGGRKLEGLD
                     FPHLLESAPLPLRTAIQGNGDTLFTMEVEGEPQVYHLSMRHFHLNARPQQLILLKQLT
                     RELAAQEVRIWKKVIRVIAHELNNSLAPISSLAHSGQLLAQDPQPAQLQRVFTTIGER
                     AAHLGSFIEGYSRFAKLPRPRPVPTDWSHLLARLEGAVAFRLDGPLPRRAVSLDPSQI
                     EQVLINLLKNAAESGSAPEAIQIRVRDGGEGFVLEVADRGSGLSEAVLRDALLPFYST
                     KPTGSGLGLTLCREIVEAHGGRLSLANRPDGPGAVVTLWLP"
     gene            complement(9058..10155)
                     /gene="galU"
                     /locus_tag="VFB37_09120"
     CDS             complement(9058..10155)
                     /gene="galU"
                     /locus_tag="VFB37_09120"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZO22654.1"
                     /translation="MRTHHRSTIRTAVFPVAGRGTRFLPATKASPKEMLPVVDKPLIQ
                     YAVEEALAAGATRLVFVTGASKRAIEDHFDSDQELEHMLEAQGKSDLLNQLRSVLPSY
                     ASCIYIRQPAPLGLGHAVLCAQPAVGAEPFFVHLADDLIRSEVACLRQMADVYEAKRA
                     SVLGVEMIPNRDTEKYGIVAVEADKSATSRVRSIVEKPKPAQAPSNLAVVGRYVLMPS
                     IFEHLEKIGHGAGGEIQLTDGIAALMREEAVYAHRFTGKRYDCGSKLGYLQATVEFAL
                     GHPQLGREFGRYMQTLSRATINSIAARGNDYVQPDRRAVNGEATLAQSGGASEKPAAQ
                     NGKGARNGKGSRNGKAGARRKGKSARMPPPV"
     gene            complement(10316..11458)
                     /locus_tag="VFB37_09125"
     CDS             complement(10316..11458)
                     /locus_tag="VFB37_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO22655.1"
                     /translation="MIELPPILLALAFIAVFFSAWILGRRNGTARTVRLPRDYYVGLD
                     HLINDRFDHAAEILSRMAEADGDAAEIQFALGSLFRRRGEVDRAIAIHSRLREHGGAA
                     VRDKAAFALAQDYLSAGLMDRAERLLEELAASREYRHAALDHLVRIYEQQGEWANALK
                     VFHDLPPALQMERRTVAAHYLCELAEHALLQGDTSRAQTLLRQARTHDAELPRAQILT
                     ARMLELAGDANGSLTLYLTALEAFPGLALEIIPRVLSLAKLVGEADVLAQLTRRLQRN
                     GHVSGRQLAWLLATALPPQEVRELPMVTREFGAGAAATADAASLGALLTRIGDAGGRY
                     QCDDCGLHSVGWYWHCPKCRSWDSMRPAVFKWAERTDDAAAHAVHG"
     gene            complement(11540..11842)
                     /locus_tag="VFB37_09130"
     CDS             complement(11540..11842)
                     /locus_tag="VFB37_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HZO22656.1"
                     /translation="MTKSELIEIITAKQKHLPAKDVELALKQILEIMSDALSQGERIE
                     IRGFGSFSLHFRPPRQGRNPKTGEAVALSGKYVPHFKPGKDLRERVNDGADRPIRD"
     gene            complement(11872..>13316)
                     /gene="rpsA"
                     /locus_tag="VFB37_09135"
     CDS             complement(11872..>13316)
                     /gene="rpsA"
                     /locus_tag="VFB37_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZO22657.1"
                     /translation="TGETRLSREKAKRARTWTRLETAFNNSEIVTGVITGRVKGGFTV
                     EIDNVRAFLPGSLVDVRPVRDTSYLENKPLEFKVIKLDQKRNNVVVSRRAVVEQEFSA
                     ERSALMDNLQEGAVVRGSVKNLTDYGAFVDLGGIDGLLHITDMAWKRVKHPSEVVKVG
                     DEIDVRILKFDRERSRVSLGLKQLGADPWENISRRYPPHTRVFGKVTNIADYGAFVEI
                     EDGVEGLVHVSEMDWTNKNVNPAKVVHTGQEVEVMVLDVDEERRRISLGLKQCNANPW
                     KEFAENYNRGDKVSGQIKSITDFGIFIGLPGNIDGLVHLSDISWDQPGEEAVRNYQKG
                     QQIEAMVLSIDPERERISLGIKQLAKDPFSAYIAENPKGTIVRGVVKEVDARGAVIDL
                     GNGIEGQLRASELGRDRVEDARSVLKVGEAVEAKFTGVDRKSRTISLSIKAKEMHEEA
                     EAVQSYRSETAPSGTSLGELLKEQIGSDRS"
BASE COUNT         2278 a         4448 c         4186 g         2404 t
ORIGIN      
        1 tatgccagtg tcggttacct ctacgactac ccacatcacg tgcatccgag cgaggatcag
       61 atcaaggctg ctctgcccgc cgtcaactgg cgcaacctga ttttcgacga gtcacaccac
      121 agcatccgct tcggccgtcc gggcatgcgc atcgacctgg ggggcatcgg caagggctac
      181 gccgtggatc gcggtatcaa cattcttcag gcgcgcggca tccaacacgg tctcgtgacc
      241 gccggcggtg attcgcgcat catcggcgat cgtatgggaa ggccgtggct cgtcgggatc
      301 cgcgacccga acgatgccac gaaggtcgtg acccgcattc cgctcgtcaa ctcagccatg
      361 tccacctcgg gcgactacga gcggtacttc gatgagaacg gtgtgcgcta ccatcacatc
      421 atcgatcccc acacgggcca ctcagcgagc aaggtgcgca gcgcgacgat cctcgggcca
      481 accgccacgc agactgatgg aatgtcgaag acggccttcg tgctcggacc cgagaaggca
      541 ctcgagatca tcaaccgcat gccggagtac gatgcgatat tcgtcactcc ggacggccgt
      601 atcctctatt cgaacgggct gcggccaccc aagccgcgcg aggcggccag agcgcacctc
      661 aacccttagg gctcagcttc gggacttccg cgggtgcgct cgctgtggcc gccgcagcga
      721 ggttactgcg atgtgccgcc gtggcgtgcc ttcagcaggg cgtccacctc gagctcggcg
      781 gcgatccagt cttccctttc attcccgggc gcaaagcccc gacgctccgc gcggaggtat
      841 gccgcctctg cgatcatcgc gcgccggaca tcttctgtga tcgcgaccga ggccggcggc
      901 atggcgggct ttttcacccg cggcgatgac ggcgcagcag ccttgggcgt ggacggggcc
      961 gcagccttgt catcggcttt gatgaccttc ggaatttcct taggaatctc gggcttgcgg
     1021 gttatatcgg tgggacggcg agtggccatc ggctcctcca agttgatgtc gagagaagct
     1081 tgaagcgcca ggccccactc gcgcaagcca ggcccacagc ctgtcggcag ccgggcgacg
     1141 cgtcggttcc caaggggcca cctggcgcgt ccgacacacc tcaccgcgct gtccgcgcgg
     1201 tgtccgctgc tgcgtccgct gtccgtccgg cggacagcca gacaggaaga aaacccgctc
     1261 gcctcgcgca ataagccctc gcagttcagc ggtttgcagg cggtgggcgc aggtggcacg
     1321 ccccgtgcaa acagcctgtc acaaacaggt gccctgcatg aagattgccg aactcaagcg
     1381 aatgtcccgc ccagcctcgc ccgcagtccc ggttcgcggg accgagcagc aggatgttgc
     1441 gatcgccgga tcggctgcgc cctggcacaa acgtcccgcc gttttgtatc cggcgctcgc
     1501 cggcgccctc gtgctcatca ttgtcatcgg ctggctcatc agagcctggt tcaactccgg
     1561 ccatgtggtc tccgccgaca gattgcagat cgctaccgtc tcgagtggtc acttcgttcg
     1621 cgatgtcgca gcacagggca ctgtcatcgc cgccgtgagc cctactctct tctcgatcgc
     1681 gccgggaacc gtgagctacc tggtgcgcgc tggggacacg gtcaccaagg gtcagatcgt
     1741 agccaccctc gacagcccgg agctcaacaa cgagtaccag cgcgagcgag cgacgctcga
     1801 cagcctcaat gcagccctcg agcatcagca gatcgaaatc cgccgccagc tcctgacgag
     1861 caagcagcag gcggacctgg cgcaggtggc catcaacgct gccgagagag agcgcaagcg
     1921 cgccgactgg gcctggggcg agcgcgcgat gagcgagcgt gattaccgtc gcgccatcga
     1981 cgacgtctcg accgcaaagc tgaatttcga gcacgcgcgc gatacggcaa aactcgaacg
     2041 cgaaagtgtg gtgctcgatc tgcgcacccg gcgtctcgag cgggaccgcc aatcgctcgt
     2101 ggtcgacaac ctcgcgcggc gcgtggctga gctcaatgtc cgctcgccgg tcagcgggat
     2161 ggtggccaac cttgcccagg cggagaagac tcgggtcgcc gaagggaccc cgctgatcac
     2221 cgtggtggac ctgaccgcct tcgagctcga attcgaggtt gccgagatct acgcgcgcga
     2281 tatcaagacg ggcatgcccg ccgagatcac gctcaacggg cacattcagc cgggtacggt
     2341 caccgccatt tccccggaag ttcgccaggg ccaggtcgtc ggccgggtgc gcttcagcca
     2401 cggccagccc ccggggctgc gccagaacga gcgcgcagcg gtgcggatcg tgcttgatga
     2461 gcgcgatcat gtcctgaagt ttgaacgcgg cccggtcatc gacgaaacga cgcgctatgt
     2521 gtatgtcgtg cgcggcaacc gggccgttcg cacccccgtg cagctcggcg cggcgtcggt
     2581 ctcggagatc gaggctctcc acgggctcgc tccgggcgat cgggtcatca tctccgatac
     2641 gcgcgactac aacgacacac ccgagctgct catctccaat tgaaggtttt cccatgctca
     2701 acatgcaaca cgtcggcaag gtctaccgga ccgatctcat cgagacacac gcgctgcgtg
     2761 acttttctct ggaggtgacc gctggcgaat tcgtcgcggt caccggaccg tccggctcgg
     2821 gcaagaccac cttcctcaac gtcgcgggcc tgctcgagac gttcgaaacc ggcacctaca
     2881 gcctcgatgg cagcgacgtg actcgtctgt cggatgatgc gcgctcgcgg ctgcgcaacg
     2941 agaagatcgg cttcatcttc caaagcttca acctgatccc ggacctcaac atccacgaca
     3001 atgttgacgt accgttgcga taccgcggcc tgcaggcctc cgatcgtcac gagcgcatct
     3061 cgagcgcgct cgagctcgtc gggctggcct cgcgcaccaa gcacatgccc tcgcagctct
     3121 cgggcgggca gcagcagcgg gtggccatcg cgcgggctat cgccggcgat ccggcattcc
     3181 tgctggccga tgagcccacg ggcaacctcg actcgctcat ggcgcggcag gtgatggagc
     3241 tgctcgagca gatcaacgag atgggcacga cgatcgttct ggtgacacac gatccggagc
     3301 tcgcgcgccg ggctcaccgt aacgtgcatg tcgtggacgg ccagatcacg gacttccgcg
     3361 cctacgagtc gcgccccgtc accgcgagca ccgatggcgc cggcagcgcg ctcggctgag
     3421 cagtcaagga gcatccatgt tcggctacta caccgccctc gcgttccgca gtttcaggcg
     3481 caatcccggc atcaccgccc tgatggtgat cgcggtggcc ctcggcatcg cggtgtgcgt
     3541 gatgacgctc accgtctatc acgcgatgtc aggcaatccg atctggtgga agaacgaccg
     3601 cctgtacgcc gtaaccatgg acaactggga tcccaatgtt ccgttcttcc aggactcgag
     3661 cctgccgccc cccgagctga cctacaagga cgcccagtat ctgttccggt cgaatattcc
     3721 ggaacgcaag gtcgtcatgt acgccacgga cggtgtgatc agcggcgcgg ggtcgtctcc
     3781 ggcggagcac gtcaagacac gtgtcacgac ggccgacttc tttgcgatgt tcgacgtacc
     3841 cttcctgtac ggctccggtt ggcgtggcgc cgcggacgat ccggcgcaac cgctgatcgt
     3901 gctgtcgcgg gaagaaaacg acaagctgtt cggcggtgcc aacagcgttg gcagagtgct
     3961 gcgctggaac gaccacgagt tccgtgtttt gggagtcctg aatgactggg cgccgcggcc
     4021 tagattttat gacctgaatg gcggcagctt cggctcgccg gacgccgtct atatccccta
     4081 cggatggggc gctgagctcg agctgttcaa tgacgaggaa agcgactgct ggaagtcgga
     4141 gaaaatcgat accttcaaag actatttggg ctccgaatgc atctggcagc agatgtgggt
     4201 cgagctcccc agccgggcca gccgtaatcg catgcagtcc ttcatggacg gctattgggc
     4261 ggagcagcgc cgctcggggc gcttccagcg cccgcgcgac aaccggctga cgaatgtggc
     4321 ggactggctg cgagacaacc aggtggtccg caacgacaac cggctgctgg tcgcgctggc
     4381 gttcgcattt ctcgcagtgt gtctgatcaa cacggtaggg ctgctgctcg cgaaattcct
     4441 caatggcgcg agcgttaccg gcgtgcgccg cgccctcggg gcgagtcgac gacagatatt
     4501 catccagcac ctggtggagg tcggggcttt gtccgccctc ggagcgctgc tcgggctcgg
     4561 tttcgcctgg atgggcctgg ccggcgttca tcacctgtat gcatcggctc atctgggccg
     4621 gcatggcggc tatcaggagc tgacgcattt cgattccgtg ggaatcctct gggccatcgt
     4681 cctggcgatt ggcgccacct tggcggcggg gctgtacccc gcatggcgga tcggacgatt
     4741 acccccggcc gtgtacctga agagccaatg agcaggccgg ccgaggccgc ctgagaagga
     4801 accaacatga gtcttcacgt ccgaccgatt ctttcagcgc tgctgcgcaa tcgcaccggt
     4861 gcggtcctcg ttgccatgca agtcgcgatc acgctcgccg tgctggtgaa tgcgctgtac
     4921 atcgtggtcc aacgcgcgca gaagatcgat cggccgaccg ggattgatct gcagaacatt
     4981 tttgtgatcc agagtgccgg cttcacgcag cgctttcaga tcagcccctc attgcaggaa
     5041 gatctcacct ggctgcgcga gctgcccggc gtgatcgcgg cgacgcccag ctcggtcatt
     5101 cccctgtcca acggaggcaa caacgagccg atcgtgacgc ggccggatct gcagcgcagc
     5161 gagtactaca atgagatcaa cgtcgacgag cacggactgg atgcgctggg ggcgcacctg
     5221 gtggccgggc gcaatttccg ccgcgaggaa atcagcccgc ctcgaggcaa gaaagacgcc
     5281 tcgcgcttcg aaccggagat catcgtcagc cgggcgctgg ccgagcggct gttccccgcg
     5341 cagaatccgc tcggaaagac ggtttacgac ttcatcaacc agacggccac catcatcggc
     5401 gtcgtcgatc cgatcatggg gtgctttccc tcgcacgaac acccggactg ggtgttcttc
     5461 gtgccccgcc tgccctcggg cgaggcagcg gtgaactatc tggtccgcac ccggcccggc
     5521 cagcgcgata gcgtcatgcg actggcggaa gcgcacctca gcaaatcgaa cccggaccgg
     5581 gtcatcgact cggtgcgccc gcttacttac tatcgcaatc tcagttatct cgacgaccgc
     5641 tccatgcaga tctatctgct tacggtgacg gtcctgctga tctctgtcac ctgcctcggt
     5701 attttcgcac ttgccacgtt caacgtgagc agccgcacca agcagatcgg tacgcgccgc
     5761 gcggtcgggg cacggcgtcg cgacatcgtg cggtacttcc tcgtggagaa tgcactcatc
     5821 acctcggcag gcgttttgac cgggtgcgct ctggcgctcg gggcgggcta ctggctgtcg
     5881 gtcgagtatg cactgccgcg tctcgatctc tattacctgg tcggaggtgt cctcgtactc
     5941 tgggccatcg ggcaactcgc ggcctgggag ccggcgcggc gggcggcggc cgtgtctcca
     6001 tcggtcgcga cgcgaacggt ttgagccggg acgccaaggc cctgtgccgc gccacgcccg
     6061 gccccgagcg cggctgcgga tctttcggcc aggtaggacc ccacctcgcg cgcggcacag
     6121 ttcctgttcc ggtcagcgtc cttaaacgct aaagcggctg ctagcatcca agccatgacc
     6181 ttcagcaaca tcggctcggt gcgctccagc ttggcgaaca ggaccgtcct cgtcatcgat
     6241 gacagtgagg ccgtgcggac ggccttcgat gtgctgttgt cgctccatgg ggctcgggta
     6301 ttgaccgcgg ccacgcccgc ggacggactc gctctgttgg cgcccgacgc ggtcgacctc
     6361 gtcattcagg atatgaactt cagacgggag gcgaccagcg gtgaggaggg gatcgcgctg
     6421 tttcacgaga ttcgccagcg ctctccagag ctgccgatca ttcttctgac cgcctggacg
     6481 cacctcgaga ctgcggtcga gctggtgaag gcgggagctg cggattacgt cgccaagccc
     6541 tgggatgatg cccgcctgct gaccgccgtg cgtaatctcc tggatctgca aagcgcccgg
     6601 gcggaagctc gcgccatgcg cgagcagcga cgcgaggccc gcgaggctct ggccgagcgc
     6661 ttcgatctgc gcgggatcgt ttacgaaagt gaggcaatgc ataccttgct cgccaccgcc
     6721 acacacgtgg cgcaggcgga cgtgccgatc ctgattaccg gccccaatgg agtcggcaag
     6781 gaggtgctgg ccgacatcgt gcaggccaat tcggcggtac gtgacaggcc gtttctgaaa
     6841 gtaaacctcg gggctctgcc gaacgatctg atcgaggcgg agctcttcgg cacggaagcg
     6901 ggcgcattca cgggggcacg cgcccgctct ggacgcttcg aggcggcaga cggcggcacg
     6961 ctgttccttg acgagctggg taatctcgcc ggcagcggcc aggccaagct cctgcgcgtg
     7021 ctgcagacgg gcgagttcga gaggctcggc tccaactcca cacggcgcac ccgggtacgc
     7081 atcatcgccg ctaccaacag taacctgcgt caggcgatcc gggagggccg cttccgggag
     7141 gacctcttct accgcctgaa tgtcatcgag ctggagattc cgccgctggc cgagcggcgc
     7201 gaggacattg tcgccctggc tcgacatttc ctgcagcctg ggtatgagct cagtcccgaa
     7261 gcgctccagg ccctatgccg ctattcctgg cccggtaacg tacgagagct gcagaacagc
     7321 atacgtcgcg cctgcctcct ctgcagtgag ggggtcctga aaccgggcga tctcaacctg
     7381 ccgtccgagc ccctgccggc cgctgccgaa gagcccgcgc tggatcgtat ggccattgag
     7441 caggcactcg ggcgagcgca gggggtgatc gcgcaggccg cgcgtgacct cggcctgtct
     7501 cgtcaggcac tctatcggcg catggagaaa ctgggcctca agaccgagat ggcccgcgcg
     7561 acgccgggct cctgatgcgc caattcctgg cgggatggac cctgatgctc aggtccgtcg
     7621 caaggtgccg ctgatgcgcg gggccacaag atgccattga tgcgcaggtc cacggggtgc
     7681 cgctgatgcg caggtccctc tcggggcgcg ccgccatcgt cctcgcgacc gtggccatca
     7741 tttcggcagc cgctgcggca gtggcggcgc gattctttcg ctccactctg ctgatcgcgc
     7801 tcatcggcgt tctcgtcgca acgccggcga tcctgtggct cgcccggcgc ttcacgcgac
     7861 cctggacgcg gatcctgctt gcagtgcgcg acggcattac cagcatgcgc gaccacgatt
     7921 tcagtgtcag catcagccgc atctccgacg atgagctggg cgagctggtg agcgcctaca
     7981 actcccttgg cgatctgctg cgtcgtcagc gcctggatat caatcagcgc gagttgctgc
     8041 tggacaccgt gattcaggcc accccgctgg tcatggtcct gaccgactcc gcgggccggg
     8101 ttgtctacag caacctcgcc gcgcggcagc tcctgtacgg cggccgcaag ctcgagggac
     8161 tggatttccc gcatctgctc gagagcgcgc cgctgccatt gcggaccgcc atccagggca
     8221 atggcgacac tctattcacg atggaggtgg agggcgagcc gcaggtgtat cacctctcca
     8281 tgcggcattt tcacctgaac gcacgccccc agcagctcat ccttctcaag cagctcactc
     8341 gcgagctcgc cgcccaggag gtgcgcatct ggaaaaaagt cattcgcgtg atcgcgcacg
     8401 agctcaataa ctccctcgcg ccgatctcct cactggctca ctcgggtcag ctgctggcgc
     8461 aggaccccca gcccgcgcag ctccagcgcg tgttcaccac catcggcgag cgggccgcgc
     8521 atctgggcag tttcatcgag ggatactcgc gcttcgccaa gctcccccga ccccgtccgg
     8581 ttccgaccga ctggtcccat ctgctcgcgc gtctggaagg cgcggtcgct ttccgcctgg
     8641 acgggccgct accgcggcgg gcggtgagtc tcgatcccag tcagatcgag caggtgctga
     8701 tcaaccttct gaagaatgct gccgagtcgg gctccgcgcc cgaggcgatc cagatccgcg
     8761 ttcgcgacgg cggcgaaggg ttcgtcctgg aggtcgccga tcggggcagc gggctcagcg
     8821 aggcggtcct gcgtgatgcc ctgctgccgt tctactcaac caagccgacg gggagcggcc
     8881 tgggactgac tctgtgccgc gagatcgtgg aggcacatgg cggccggctc agcctcgcca
     8941 atcgaccgga tggtccaggg gctgtcgtca cactttggtt gccgtagcag ccagctgccc
     9001 gcatcactcc ctggcggctg gcattgcaat attacgcggg ctgcgccatc acccgaatca
     9061 cacgggcggg ggcattcgtg cagactttcc ctttcgccgc gcaccggctt tgccgttccg
     9121 ggatcccttg ccattgcggg ctcccttgcc gttctgggca gcaggctttt cgctcgcacc
     9181 tccggactgc gccagcgtcg cctcgccatt gacagcgcgg cgatcgggct gcacgtaatc
     9241 attgccgcgg gccgcgatgg agttgatcgt tgcccggctg agcgtctgca tgtagcgacc
     9301 gaactcacgc ccgagctgtg gatggcccag cgcaaactcg actgtcgcct ggaggtaccc
     9361 cagcttgctg ccgcagtcat agcgcttgcc cgtaaaccgg tgcgcataga cggcctcttc
     9421 cctcatcagg gccgcaatgc catcggtaag ctggatctca ccgcctgctc cgtgcccgat
     9481 cttctcgagg tgctcgaaaa tcgagggcat caggacgtag cggcccacga cggccagatt
     9541 cgatggcgcc tgcgcaggct tcggcttctc cacgatactg cgcacacggc tcgtggcgga
     9601 cttgtcggct tccacggcca cgatgccgta cttctcggtg tcccggttcg gaatcatctc
     9661 cacaccgagg acgctcgcac gcttcgcctc gtagacatca gccatctgcc gcaggcaggc
     9721 cacctcactg cggatcaaat catccgccag atgcacgaag aagggctctg cccctaccgc
     9781 cggctgggcg cacaacactg cgtgcccgag acccaaaggg gcgggctggc ggatgtagat
     9841 acacgaggca taggagggca gaacactgcg cagctgattg agcaggtcgc tcttgccctg
     9901 agcttcgagc atgtgctcga gctcctggtc cgagtcgaag tgatcctcga tcgctcgctt
     9961 ggaggcgccg gtgacgaaca cgagccgcgt tgcgccggcg gccagcgcct cctccaccgc
    10021 atactgaatc agaggcttgt cgacgacagg aagcatctcc ttggggctgg ccttggtggc
    10081 cggaagaaag cgagtgcctc tgccggcgac cggaaagacc gccgtgcgaa tcgttgatcg
    10141 gtgatgcgtc ctcatcgagc tcctctccga gcctgtgccg cgtgagtcag tgattctact
    10201 gagccaacgc gcagcaaagt atatgccacc gcaagcactt cacgtgcgtg cgcgggcact
    10261 ggcttcgcac cacggagccc acggggcgaa tcggcggctc caatctggac ttactctagc
    10321 cgtgcacagc gtgcgcagcg gcgtcatccg tgcgctcggc ccatttgaag accgccggcc
    10381 gcatgctgtc ccacgagcgg cacttgggac agtgccagta ccagcccacg ctgtgcaggc
    10441 cacagtcgtc acattgatag cgtccgcccg catctccgat acgggtcagg agcgccccca
    10501 gggaagcggc atccgcggtc gccgccgccc cggctccgaa ctcacgtgtc accatcggca
    10561 actcgcgcac ttcctgcggc gggagcgcgg tcgccagtag ccaggcgagc tggcgcccgc
    10621 tgacatgccc gttgcgctgc agccgtcggg tgagctgggc caggacatcc gcctccccca
    10681 caagcttcgc cagcgaaagc acgcgcggaa tgatctcgag cgcgagcccc ggaaatgcct
    10741 cgagggcggt cagatacagt gtcagggaac cgttcgcatc tccggccagc tccagcatgc
    10801 gcgccgtcag gatttgagcg cgaggcagtt cggcatcgtg cgtgcgggcc tgacgcagca
    10861 gcgtctgcgc ccgtgaggtg tctccctgca acagggcgtg ctcggcgagc tcacacaaat
    10921 agtgcgcagc cacggtccgg cgctccatct gcaatgccgg aggcagatcg tgaaacacct
    10981 tgagcgcgtt cgcccactct ccctgctgct cgtagatgcg cacgaggtga tcgagcgcgg
    11041 catgccggta ctcgcgtgag gcggccaact cttccagcag ccgctcggcg cggtccatca
    11101 ggcccgcgct caggtagtcc tgagccagcg caaaggccgc cttgtcgcgc acggccgccc
    11161 cgccgtgctc gcgcaggcgc gagtggatgg cgatggcgcg atcgacctca ccgcgccggc
    11221 ggaacaggct gccaagcgca aactggattt ccgccgcatc gccatccgcc tcggccatac
    11281 gcgagaggat ctcggcggcg tgatcgaaac ggtcgttgat gaggtgatcc agaccgacgt
    11341 agtagtcgcg cggcaggcgc accgtgcgtg ccgtaccatt gcggcgaccc agtatccacg
    11401 cggaaaagaa gaccgcgatg aaggcgagtg cgagcagtat tggaggcagc tcgatcacga
    11461 cgccgacgat agcagccggg tgttacaacg tatagcggcc gcaggcgccg cgagtcctca
    11521 acagtgagtc ccggacgggc tagtcccgga tcggccggtc cgccccgtca ttgacgcgct
    11581 cgcgcagatc cttgcccggc ttgaaatgcg gcacgtactt gcctgacaga gcgaccgcct
    11641 cgccggtctt gggattgcgt ccctggcgcg gcggacggaa gtgcagcgaa aagctgccga
    11701 atccgcggat ctcgatgcgc tccccctgtg agagcgcatc gctcatgatc tccaggatct
    11761 gcttcagcgc aagctcgacg tctttggccg gcagatgctt ctgcttcgca gtgatgatct
    11821 cgatcagttc ggatttggtc attcaggtca ccgcgcgccc ccgtgaggga atcagcttcg
    11881 atccgagcca atctgctcct ttagcagctc tccgagactg gtgcccgagg gtgccgtttc
    11941 ggagcgatag ctctgcacgg cctccgcctc ctcgtgcatc tccttcgcct tgatcgacag
    12001 ggagatggtg cgcgacttgc gatcgacgcc ggtgaacttc gcctcgactg cctcgcccac
    12061 cttcagtacc gagcgggcat cctcgacccg atcccgcccc agctcggagg cgcgcagctg
    12121 tccttcgata ccgttgccga gatcgatgac cgctccgcgc gcatccactt ccttgacgac
    12181 gccgcgcacg atcgtgccct tggggttctc ggcgatgtag gccgagaacg gatccttcgc
    12241 gagttgcttg atgccgaggg agatgcgctc gcgctccggg tcgatcgaca gcaccatggc
    12301 ttcgatctgc tgccccttct ggtagttgcg cacggcctcc tcgcccggct gatcccagga
    12361 gatgtccgac aggtgcacca ggccgtcgat gttgcccggc aagccgatga agatgccgaa
    12421 atcggtgatc gacttgatct ggccggagac cttgtcgccg cgattgtaat tctcggcgaa
    12481 ctccttccac ggattggcgt tgcactgctt gaggccgagg gagatgcgcc gccgctcctc
    12541 gtccacatcg agcaccatca cctcgacttc ctggccggtg tgcaccacct tggccggatt
    12601 cacgttcttg ttggtccagt ccatctcgga gacgtgcacc aggccttcga cgccgtcctc
    12661 gatctcgacg aacgcgccgt agtcggcgat gttggtcacc ttgccgaaca cgcgcgtgtg
    12721 cggcggatag cgccgcgaga tgttctccca cggatccgcg ccgagctgct tcaggccgag
    12781 gctgacccgc gagcgctcgc ggtcgaactt cagaatgcgc acatcgatct catcgcccac
    12841 cttgacgact tccgaaggat gcttgacgcg cttccaggcc atgtcggtga tgtgcagcag
    12901 cccgtcgatg ccgcccagat cgacgaatgc gccgtagtcg gtgagattct tcaccgagcc
    12961 gcgcacgacc gcgccttcct gcaggttgtc catcagcgcg ctgcgctcgg cggagaactc
    13021 ctgctcgacg accgcgcgcc gggagaccac gacgttattg cgcttctgat cgagcttgat
    13081 gaccttgaac tcgaggggct tgttctcgag ataagaggtg tcgcgcaccg ggcgcacatc
    13141 gacaagcgag cccggcagga atgcgcggac gttgtcgatt tcaaccgtga aaccgccttt
    13201 gacacggccc gtgatgacac cggtgacgat ctcggaattg ttgaaagccg tctccaggcg
    13261 cgtccaggtg cgcgcccgct tggccttctc gcgcgacagg cgtgtttcac cggttc
//
LOCUS       DASRHN010000235         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_241859, whole genome shotgun sequence.
ACCESSION   DASRHN010000235 DASRHN010000000
VERSION     DASRHN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241859"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFB37_09140"
     CDS             <1..797
                     /locus_tag="VFB37_09140"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZO22658.1"
                     /translation="EMGDTELPPAPVSGGSNSTATVCSAVLLACKQVRRRVFQGAVGS
                     GKGPLAGRDPATLRLVNGAITSDGASQKLAEVFDGLGVAMIEEYAEFIPEGAPADAVR
                     KLYAGQSTLIGGSKGSQVKFAFGAEFVEVRIHARTREIRVPRLVGTFAAGRIMNPRTA
                     RSQLMGGMIWGMASALLEATELDPRSARTVNRDLQEYYLAVNADTPEVRITMLPEVDA
                     AVNPAGVKGIGELGDVGTNAAVANAVFHATGKRIRTLPIRIEDVGP"
     gene            complement(794..1519)
                     /locus_tag="VFB37_09145"
     CDS             complement(794..1519)
                     /locus_tag="VFB37_09145"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZO22659.1"
                     /translation="MQYRLYYWPSIQGRGEFVRLALEEAAADYIDVARQPGKGFGIPA
                     LMRLMDARRVEHAPFAPPFLQAGQQIVGQTANILLFLGARHALAPRSASGRLWIHQLQ
                     LTIADFVAEAHDTHHPVAAGLYYEDQKPEARRRAEDFRNSRMPKFLGYFEHLLAHNTR
                     GPYLCGARLSYADLSVFQMVAGLDYAFPRAMASAARHYKRILTLSRAVQDRPRIAAYL
                     ASDRRIPFNQEGIYRQYPELDAR"
     gene            complement(1594..2559)
                     /locus_tag="VFB37_09150"
     CDS             complement(1594..2559)
                     /locus_tag="VFB37_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22660.1"
                     /translation="MTVSRDLLAAIDLLPEPMVLVRVEGEILAVNVAFARQMGRSRES
                     FVGTSLEAVLADPWREVGDYLGACARSERLVAGSLTLRHPDGATLEFLAGGAGYAVGE
                     GARTEPAESVFLRLEARTDSAGFLAVLAHELRNPLAPIRTALEVMRVSAGDQAVATAA
                     RSMIERQLQQLVRLIDGLTTRPADAAVAPEPAAKRKPAPVRARILVADDNRDAAQSLA
                     FMLEIEGHDVRTAHDGVEAVELAEAFRPQLALLDIGMPRQDGYAAARELRGKAWGGSV
                     CLVALTGWGQEEDRLRAQEAGFDRHLVKPVDPKILNALIAQVLQT"
BASE COUNT          465 a          899 c          880 g          420 t
ORIGIN      
        1 ttgagatggg cgacaccgag ctgccccctg ctccggtctc gggcggctct aacagcacgg
       61 ccaccgtctg ttctgccgtg ctcctggctt gcaagcaggt gcgccggaga gtgttccagg
      121 gggccgtcgg ctcaggcaaa ggtccgctcg ccggacgcga cccggccaca ctgcggctgg
      181 tcaacggcgc aatcacctcg gacggagcct ctcagaagct cgcggaagtc ttcgacggtc
      241 tcggggtcgc gatgatcgag gaatacgccg agttcattcc ggagggagcc cctgcggatg
      301 ccgtgcgcaa gctctatgcg ggacaaagca cgctcatcgg aggctcgaag ggcagccagg
      361 tcaagttcgc cttcggcgcc gagttcgtcg aagtgcgcat ccacgcccgc acgcgcgaga
      421 tccgcgtgcc gcgtctggta ggcacgttcg cggccggccg catcatgaat ccgcgcaccg
      481 cgcgcagcca gctcatgggc ggcatgatct ggggtatggc ctcggcgctg ctggaggcca
      541 cggagctgga cccgaggagc gcgcgtaccg tcaaccgcga cctgcaggag tactacctcg
      601 cagtaaatgc cgacacgccg gaggttcgca ttaccatgct gccggaggtg gatgcggccg
      661 tgaatcccgc aggtgtcaag ggaatcggtg agctgggcga tgttggcacg aatgctgccg
      721 tcgcaaacgc cgtgtttcac gcgaccggca aacggatccg cacgctgccc atccgcatcg
      781 aggacgtagg gccttagcgc gcgtcgagtt ccgggtactg gcggtagatg ccctcctggt
      841 tgaaggggat ccggcgatcg gaagccaggt acgccgcgat gcgcggacga tcctgaacgg
      901 ctcgcgagag tgtcaggatg cgcttgtaat gacgcgcggc gctcgccatg gctcgtggaa
      961 atgcatagtc gagcccggca accatctgga agacagacag gtccgcgtag ctcagccggg
     1021 cgccgcacag gtacgggccg cgcgtattgt gcgcgagcag atgctcgaaa tagcccagga
     1081 acttcggcat ccgggaattg cgaaagtcct cggcgcgccg gcgcgcctcc ggtttctgat
     1141 cctcgtaata caggcctgcg gcaaccggat ggtgcgtgtc gtgcgcctcg gccacgaagt
     1201 ctgcgatcgt gagctgcagt tgatgaatcc agagccggcc cgatgcgctc cgtggcgcca
     1261 atgcatggcg cgcgccgagg aacaggagga tgttggccgt ctggcccacg atctgctggc
     1321 ccgcctgcag gaagggcggt gcgaagggcg cgtgctcgac acgccgtgcg tccatcagac
     1381 gcatcagcgc cgggatgcca aatcccttgc cgggctgccg tgcgacatcg atgtaatccg
     1441 ccgcggcttc ctcgagcgcc aggcgcacga actcaccgcg cccctgaatt gagggccagt
     1501 aatagagtcg gtactgcatc gcggatcctc accggatcaa ctcattcagc cgcgatctgt
     1561 tccgcgcaac gcaccgcagg tgcgcgattg cccctacgtc tgcagcactt gagcgatcag
     1621 ggcgttcagg atcttcgggt ccaccggctt caccagatgc cggtcgaagc cggcctcctg
     1681 cgcgcgcagg cgatcctcct cctgtcccca gccggtcaaa gcgacgagac agaccgatcc
     1741 gccccatgct ttgccccgca gctcgcgggc cgctgcgtag ccatcctgcc ggggcattcc
     1801 gatatcgagc agcgccagct gcgggcgaaa agcctcggcc agctccaccg cctccacccc
     1861 gtcatgcgcc gtccgtacat catgaccttc gatctcgagc atgaaggcga ggctctgcgc
     1921 cgcatcccga ttgtcatcgg ccaccaggat ccgggcccgc accggcgctg gcttgcgctt
     1981 cgccgcgggc tctggcgcaa ccgccgcgtc cgccggccgg gtcgtcaggc catcgatgag
     2041 tctgacgagt tgctgcagct ggcgctcgat catggaacgc gccgccgtcg cgaccgcctg
     2101 atcaccggcc gagacccgca tgacctcgag cgcggtgcga atgggcgcga ggggattgcg
     2161 caactcgtgg gccagcacag ccaggaaacc cgcactgtct gtcctggcct cgaggcgaag
     2221 gaacaccgac tctgcaggct cggttcgcgc gccctcaccc accgcatagc ctgcgccgcc
     2281 tgccagaaat tcgagcgttg cgccgtccgg gtgcctgagc gtgagcgagc cggccaccag
     2341 ccgctcgctg cgggcacatg cgccgagata atcgccgacc tcgcgccacg gatcggcgag
     2401 cacagcctcc agcgacgtgc cgacgaaaga ctcccgcgag cggcccatct ggcgcgcaaa
     2461 ggccacgttg accgccagaa tctcgccctc gaccctgacg agcaccatcg gctcgggcaa
     2521 cagatcgatc gcggcgagga ggtcccggct gacagtcaag gcacgacggg gatagcgcgc
     2581 agcagggctg caggtagttg cgcacgctac cagctttgcc gaagccggta cactgcaaca
     2641 agttggtggg gagctgccgg tcgg
//
LOCUS       DASRHN010000236         6312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2057483, whole genome shotgun sequence.
ACCESSION   DASRHN010000236 DASRHN010000000
VERSION     DASRHN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057483"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFB37_09155"
     CDS             <1..1354
                     /locus_tag="VFB37_09155"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZO22661.1"
                     /translation="IDCLGPVAALMAQLPGVQRVIVIPYLSSEPRLPQLAGPDNRVVR
                     FDQFGRRGAPLSFASLPFDHPLYILYSSGTTGIPKCIVHGAGGTLLQHRKEHVLHTDL
                     KRSDRLFYFTTCGWMMWNWLVSALASGASLILYDGSPFHPRPSSLWKMAQEESISIFG
                     ASAKYISTLAKSSYVPARETELPALRTILSTGSPLLPDAFDYVYDAVKSDVQLASISG
                     GTDIISCFALGSPVLPVHRGEIQCRGLGMRVEVLDETGRAVRGERGELVCRAPFPSMP
                     IGFWDDPDAVRYRAAYFERFPGIWHHGDYASLTENDGLVIHGRSDAVLNPGGVRIGTA
                     EIYAAVETLPQVLESLAIAQEWRADVRIVLFVRLRPDVLLDELLRKTIRDTIRVHASP
                     RHVPARIIAVPDIPRTLSGKLTELAVRSVVHGQSVRNLDALANPEALEHFRNLPELAS
                     "
     gene            1363..2484
                     /gene="zapE"
                     /locus_tag="VFB37_09160"
     CDS             1363..2484
                     /gene="zapE"
                     /locus_tag="VFB37_09160"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HZO22662.1"
                     /translation="MDLLALYAQELAQRGFTRDPAQQAALARLDELRSGLIGAAQARP
                     SRLRRWLDGPRSAYTEVRGLYLWGGVGRGKTWLMDLFFESLPFPRRQRKHFHRFMHDV
                     HDQLRGLQQRAAPLEAVAARIARNARVLCFDELFVSDIADAMILGGLFSALFRRGVTL
                     VATSNVPPTELYRDGLQRQRFQPTIELLEQHLDVFHMSGATDYRLRRLTQAGTYLASG
                     APDTPHRMQALFAALSDHSTTDTAALEHASAGRTIEIEGRAIPVLRENGSVVWFEFAA
                     LCSGPRSQEDYIEIARTYHTLMISGVPVFDAASDNEARRFIALVDETYDRNVNLIVSA
                     AAAPADLYRGERLAASFQRSVSRLIEMQSEEYLAREHKP"
     gene            complement(2481..>6312)
                     /gene="hrpA"
                     /locus_tag="VFB37_09165"
     CDS             complement(2481..>6312)
                     /gene="hrpA"
                     /locus_tag="VFB37_09165"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HZO22663.1"
                     /translation="ATRSAVAALRLEYDPALPITAHRAEILAALAQHPVIVVCGATGS
                     GKSTQLPKLCLEAGRASAGLIGHTQPRRIAGRALAQRVAQELGTPLVSGLVGYQVRFT
                     DRTGPATRIKLMTDGILLKELEGDRHLRRYDTLIIDEAHERSLNIDLLLGVLKQLAAE
                     RPELRLIVTSATIDPGRFAEFFGGAPVIEVSGRSYPVEVRYRPLASDPDEPAETPGAE
                     QSTELSLPQGIIAAVRELDASGRGARGDTLVFLPGEKDIREVADALRRERLPETEILP
                     LFARLSTRDQERIFAGHTQRRIVLATNVAETSLTVSGVRFVIDSGLARISRYSVRAKV
                     QRLPIERISRASADQRSGRCGREAPGICIRLYSEEDYLQREQYTPPEVLRTNLASVIL
                     RMAVLELGEPEHFPFLDAPESRLVNDGVRLLQELKAMDEARRVTSLGQKIATLPVDPR
                     LGRMLLAASHHHCVSEMLTVSAFLEAQDPRERPQEAGQQAAEKQAAFADPRSDYVTVL
                     NLWRLYEQQSQTLSGNQLRKWCRENFLSFVRLREWQELREQLRRNVLELRLPLNRIPA
                     SYSGLHQAILTGFLGSLGELDEKREYTGPRGIRFVIAPGTPLHARPPRWIVAGSLLET
                     TRLYARMVAAVEPAWIEAAGAHLLKRRYSEPHWVPRRGFVGAYESVSLFGLLLVARRR
                     VNYGAIAALEARDIFIREALLAENGSVSGDFLTHNRAVRAQVEDLEARIRRRDILVDE
                     QALVDFYARRVPGRISSVSAFEHWRAAAERKNPAILQMGLADVMRREVPDASPELFPE
                     SLPLGANRLPLRYAFAPGEPGDGVTVVVPELLVASLRPEPLEWLVPGWRLEKITAVFR
                     ALPKTLRKQLVPVPDSAQAAMQELRSDSPAGFYEGLASWISQRTGTAITAGELRALKL
                     PEHLRMNVRVLDAMDRVIEEGRDIAALKRRIHSESGVLVQPEARSVMLHRQWDFEELA
                     ESVSIARNGFSLQVFPAIEDCGRAVALVEARSPEDAEAITHAGLTRLAMLMLPQQAKY
                     VEKRMADNRELVLLSGGLAGAGLIEAMTWRAFYECFFPAGEMVPRTRQAFALTLEKRR
                     AELSAVADRLALTVVEVLRQWREVRSALQELRTPAFVAAVADIQSQLAALLPADFIES
                     VPQPWLGHLPRYLKAIARRIERLPSNTRRDAELAAKVRPFDAAVAKLLEASQGCAMLQ
                     GSKAPQASSEPRSAGIRRAIPASLAQLRWMVEEFRVSLFAQDLKTLVKVSENRLADQL
                     AQARSAMGAAEPAG"
BASE COUNT         1091 a         2146 c         1988 g         1087 t
ORIGIN      
        1 aatcgactgc ctgggaccgg ttgccgcgct gatggcgcaa ctgccgggag tgcagcgcgt
       61 gatcgtcatc ccctatctga gctccgagcc gcggcttcca cagcttgccg gccccgacaa
      121 ccgtgtcgtc cgcttcgatc agttcggccg ccgcggcgcg ccgctgagct tcgcgagcct
      181 gccgttcgac catccgctct acattctcta ctcctccggc acgacaggaa taccgaagtg
      241 catcgtccac ggtgccggcg gcacgctgct gcagcatcgc aaggagcacg tgctgcatac
      301 cgatctgaag cgcagcgacc ggctgtttta tttcaccacc tgcggctgga tgatgtggaa
      361 ctggctcgtc agtgccctgg cgagcggggc gagcctgatc ctgtacgacg gcagcccctt
      421 tcacccgcgc ccgagcagcc tgtggaagat ggcacaagag gagagcattt cgatctttgg
      481 agccagtgcc aagtacatct cgactctcgc aaagagcagt tatgtaccgg cgcgcgaaac
      541 tgagctgccg gcactgcgga cgatactgtc taccggctcg ccgctgctcc ccgatgcgtt
      601 cgactatgtt tacgacgccg tgaaatccga cgtgcagctc gcctcgatct ccggaggcac
      661 ggacatcatt tcgtgcttcg ccctcgggag tcccgtgctg ccggtgcatc gtggggaaat
      721 tcaatgccgg gggcttggaa tgcgtgtcga ggttctcgac gaaaccggtc gcgcggtgcg
      781 cggtgagcgc ggcgagctcg tctgtagggc gccgttccct tcgatgccca tcgggttctg
      841 ggatgacccg gacgcagtcc gctaccgcgc agcctacttc gagcgcttcc cgggcatctg
      901 gcatcacggc gattacgcct cgctcaccga gaacgatgga ctcgtgatcc acgggcgctc
      961 cgatgcggtg ttgaatccgg gcggcgtgcg catcggtacc gcggagatct atgccgccgt
     1021 ggaaacgctt ccccaggtgc tcgagtcact ggccattgcc caggagtggc gcgccgatgt
     1081 gcgcatcgta ttgttcgtgc ggctcagacc cgatgtgctg cttgatgagt tgctgcggaa
     1141 gacgatccgc gacacgattc gcgtgcacgc cagtccacgg cacgtacccg cccggatcat
     1201 cgccgtaccc gacattccgc gcacactttc aggaaagctc accgagcttg cggtgcgcag
     1261 cgtggttcat ggccaatcgg ttcgcaatct cgatgcgctc gcgaaccccg aagcgctgga
     1321 gcacttccgc aatctgcccg agctcgcaag ttgatgccag caatggatct actcgcactc
     1381 tatgcgcagg agctggctca gcgtggcttc accagggatc cggcccagca ggcggcgctg
     1441 gcaagactcg atgagctgcg cagtgggctg atcggtgcgg cgcaagccag gccttcccgc
     1501 ttgcgccgct ggctcgatgg acctcgatcc gcctacaccg aggtgcgcgg gctgtacttg
     1561 tggggaggcg tcgggcgcgg caagacctgg ctgatggatc tgttcttcga aagccttccg
     1621 ttcccacgac ggcagcgcaa gcactttcat cgtttcatgc acgacgtgca tgaccagctg
     1681 cggggcctgc agcagcgagc ggcgccactc gaagcagtcg cggcacgtat cgcccgcaac
     1741 gcgcgagtgc tctgcttcga tgagctgttt gtctcggaca tagccgatgc gatgattctc
     1801 ggcggcctgt tctcggcact tttccggcgc ggcgtgacac tcgtcgccac ttccaacgtg
     1861 ccgcccaccg agctctatcg agacggcctg cagcgccagc ggtttcagcc caccatcgag
     1921 ctgctcgagc aacatctgga cgtgttccat atgagcggag cgaccgatta tcggctgcgc
     1981 cggctgaccc aggcgggcac ctacctcgca tcgggggcac ccgacacgcc gcatcggatg
     2041 caagcgctgt tcgccgcgct gtcggatcac agcactaccg acaccgcggc tctggagcat
     2101 gcttccgcag gccgcacgat cgagatcgag gggcgagcga tcccagtgct gcgcgagaac
     2161 ggcagcgtcg tgtggtttga gttcgcggcc ctgtgctccg gcccgcgcag ccaggaagac
     2221 tacatcgaga tcgcgcgcac ctatcacacc cttatgatct ccggcgtacc ggtgttcgat
     2281 gcggcaagcg acaatgaggc gcggcgcttc atcgccctgg tggacgagac ctacgaccgc
     2341 aatgtgaatc tgatcgtgtc cgcagccgcc gcacctgccg atctgtatcg cggcgagcgg
     2401 ctcgccgctt cattccagcg cagcgtgagc cggctgatcg agatgcagag cgaggagtat
     2461 ctcgcccgcg agcacaagcc ctagcccgca ggctcggccg cacccatcgc gctgcgcgcc
     2521 tgcgcgagct gatcggcgag tcggttttcg gagaccttca cgagggtctt caggtcctgc
     2581 gcaaacagtg acacccggaa ctcctcgacc atccagcgaa gctgcgcgag cgaggcagga
     2641 atggcacgtc tgatgcccgc agatcggggc tcggaagaag cctgcggcgc tttggacccc
     2701 tgcagcattg cgcagccctg cgatgcttcg aggagctttg ccaccgcggc gtcgaacggc
     2761 cgcacctttg cagccagctc cgcgtcccgg cgcgtgttgg aagggagccg ttcaatccgg
     2821 cgggcgatcg ccttgagata gcgcggcaag tgacccagcc acggctgggg caccgactcg
     2881 atgaagtccg cgggcaacaa ggccgccagc tgggattgga tgtcggcaac agcagccacg
     2941 aacgccggcg tgcgcagctc ttgcagcgcg ctgcgcacct cccgccactg tcgcaatacc
     3001 tccaccacgg tcaacgccag tcggtccgcg accgcactca actctgcgcg acgtttctcc
     3061 agcgtgagcg cgaaagcctg ccgggttcgc ggaaccattt ctcctgccgg gaaaaagcat
     3121 tcgtaaaacg ctctccaggt cattgcctcg atgagccccg cgcccgccag cccaccgctc
     3181 agcaggacca gctcgcgatt gtcggccatc cgcttctcca cgtacttcgc ctgttgcggc
     3241 agcatcaaca tcgcaaggcg tgtcaggcca gcgtgcgtaa tcgcctcagc gtcctcgggg
     3301 cttcgcgctt cgaccagggc gacggctcgt ccacaatcct cgatggccgg gaaaacctga
     3361 aggctgaatc cgttgcgcgc aatgctcacg ctttctgcca gctcctcgaa gtcccactgg
     3421 cgatgcagca tgacactgcg cgcttcgggc tgcacgagca cgccgctctc gctgtgaatc
     3481 ctgcgcttca gagctgcgat atcgcggccc tcctcgatca cgcggtccat cgcatccaac
     3541 actcgcacgt tcatgcgcaa atgctcgggc agcttcagag cgcgcagctc gcccgcagtg
     3601 atcgccgtgc cggtgcgctg gctgatccag gacgccagcc cctcgtagaa tccggcaggc
     3661 gagtcactgc gcagctcctg catcgccgcc tgggcgctgt ccgggaccgg aaccagttgc
     3721 ttgcgaagcg tcttgggcaa tgccctgaag accgcggtga tcttttccag tcgccatccg
     3781 ggcaccagcc actcgagcgg ctcggggcgc aagctcgcca ccagcagctc cggcaccacg
     3841 accgtgaccc catcgccagg ctcacccggt gcgaatgcgt atcgcagcgg cagccggttc
     3901 gcgccgagcg gcagactctc ggggaacagt tccggggacg catcgggcac ttcgcgtcgc
     3961 atcacatccg caaggcccat ttgcaggatt gcgggattct tgcgctccgc cgccgcgcgc
     4021 caatgctcga aggccgacac cgagctgatg cgcccaggca cccgtcgggc atagaaatcc
     4081 accagcgcct gctcatccac gagaatgtcg cggcggcgga ttcgagcctc caggtcttcc
     4141 acctgcgccc gcacggcacg gttgtgggtc aggaagtcac cgctcacgga gccgttctcg
     4201 gcgagtagcg cctcgcggat gaaaatgtca cgcgcctcga gcgcagcgat ggcaccgtag
     4261 ttgacgcgcc gccgggccac gagcagcagg ccaaacagcg acaccgactc gtacgccccc
     4321 acaaagccgc ggcgcggaac ccaatgcggc tcgctgtagc gacgcttgag cagatgcgcg
     4381 cctgcagcct cgatccatgc aggctcgacg gccgcaacca tccgcgcata cagacgcgtc
     4441 gtttccaaca ggctgccggc gacgatccag cggggaggcc tggcatggag cggtgtgccg
     4501 ggtgcgatga cgaaacggat gccccgtggg ccggtgtact cgcgcttctc gtccagctcc
     4561 ccgagcgagc ccagaaatcc cgtgagaatg gcctgatgga gcccggaata gctggccggg
     4621 atccggttga gaggcaagcg cagctccagg acgttccgcc gcagctgctc acgcagctcc
     4681 tgccactcgc gcagacgtac gaacgacagg aaattctcgc gacaccactt gcgcagctga
     4741 ttacccgaga gagtttgtga ctgctgctcg tagagacgcc acagattcag cacggtcacg
     4801 tagtccgagc ggggatccgc aaaggcggcc tgcttctcgg ctgcctgctg cccggcctcc
     4861 tgcggtcgct cgcgcgggtc ctgcgcttcg agaaacgcac tcacggtcaa catctccgac
     4921 acgcaatggt gatgcgaggc agccagcagc atgcgaccga gccgcggatc gacgggtagc
     4981 gtggcgatct tttgtccgag gctcgtgaca cgccgcgcct cgtccatggc cttcagctcc
     5041 tgcagcaaac gcacaccatc gttcacgagc cggctctcgg gcgcgtcgag aaaaggaaaa
     5101 tgctcgggct cgccgagttc cagcacggcc atccgcagaa tgacgctggc gagattcgtt
     5161 cgcagcacct ccggcggggt gtactgctcg cgctgcaggt aatcctcctc cgagtagagg
     5221 cggatgcaga tgccgggagc ttcgcggccg cagcggccgc tgcgctgatc ggcgctcgct
     5281 cgcgagatgc gctcgatggg caggcgctgc accttggccc gcacgctgta ccgactgatc
     5341 cgcgccaggc ccgagtcgat cacgaagcgg actcccgaca ccgtgagcga ggtttccgcc
     5401 acgttggtgg cgagcacgat ccgccgttgc gtgtgacccg cgaatatcct ctcctgatcc
     5461 cgggtggaca gccgcgcaaa caacggcaaa atctccgtct ccggcagcct ctcccgacgc
     5521 agcgcgtcgg ccacctcgcg gatatccttt tcacccggaa gaaacacgag ggtatcgccg
     5581 cgcgcgccgc ggccgctcgc gtcgagctcg cgcacggctg cgatgatgcc ttgtggcaag
     5641 gacagctccg tggattgctc ggcgccgggc gtttcagcgg gctcgtccgg gtccgatgcg
     5701 agaggccggt agcgaacctc caccggatag ctgcgtccgg aaacctcgat gacgggtgca
     5761 ccgccgaaga attccgcgaa ccgccccggg tcgatcgtgg ccgatgtcac gatgaggcgc
     5821 agctcgggac gctcggcggc gagctgtttg agcacaccga ggagcagatc gatattgaga
     5881 ctgcgctcgt gtgcctcatc gatgatcagg gtgtcataac ggcgcaagtg ccggtcaccc
     5941 tccagctcct tgagcaggat gccgtcggtc atcagcttga tccgggtggc aggccccgtg
     6001 cgatcggtga agcgcacctg atagcccacc agcccgctca ccagaggcgt gcctagctcc
     6061 tgggctacgc gctgggccag agcgcgcccc gcgatgcgcc ggggctgcgt gtgcccgatc
     6121 agtccggcgc tggcgcgccc ggcctccagg cacagcttgg gtaattgcgt ggacttgccc
     6181 gatccggtcg ccccgcagac gacgatgacc ggatgctgcg cgagtgccgc caggatctcg
     6241 gcacggtgag cggtgatcgg tagagccggg tcgtactcga gccgcaatgc cgctacagca
     6301 ctccgcgtcg ca
//
LOCUS       DASRHN010000237         6061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_902999, whole genome shotgun sequence.
ACCESSION   DASRHN010000237 DASRHN010000000
VERSION     DASRHN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902999"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFB37_09170"
     CDS             <1..607
                     /locus_tag="VFB37_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO22664.1"
                     /translation="VGRRGSFGARLTVHGVQGHIAYPQLAENPVHSLAPALAELTSRV
                     WDEGTEHFQPTSFQVSNLNAGTGAPNVIPGELKARFNLRYSPAQTPERLRETVEGILR
                     KHAVRYTIEWRVSGEPFYTAPGTLSDAVGAAVERVTGTRPRLSTAGGTSDGRFIAPLG
                     AQVVELGVVNASIHKIDEHVRIEEIALLHRMYLTLLQHLLG"
     gene            complement(608..1114)
                     /locus_tag="VFB37_09175"
     CDS             complement(608..1114)
                     /locus_tag="VFB37_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HZO22665.1"
                     /translation="MAHANRRLLNFAGFIACVAMLAYAYFVQYHQGIEPCPLCIFQRI
                     GIAALGVVFLFAGVHNPRGWGSILYAVLIAATALAAAGVAGRHLYVQSLPPGTIPSCG
                     APLDVLLQFTPVTQVIRKVLTGSGECSQINWKFLGLAMPAWVLICALLLGAVGITTNL
                     WPRRPKAR"
     gene            complement(1182..1916)
                     /gene="phoU"
                     /locus_tag="VFB37_09180"
     CDS             complement(1182..1916)
                     /gene="phoU"
                     /locus_tag="VFB37_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZO22666.1"
                     /translation="METADLSHHISRRFNEDLERVRSKVLGMGGFVEQQLHKAVAALV
                     EGDSTLGESVAMDDYQVNNMEVAIDEECSRILATRAPAAGDLRVIVAIIKTITDLERI
                     GDEGEKIGYIASRLATMERPADKYREIKHLGRVVSDMVHDALDAFARMDVESALRVAR
                     QDRLVDEEYEAIQRQAITFMMEDPRTIRRALDVMWVVRALERIGDHAKNISEYVIYMV
                     HGKDIRHTSLDDVERELREHRPGTAT"
     gene            complement(1916..3019)
                     /locus_tag="VFB37_09185"
     CDS             complement(1916..3019)
                     /locus_tag="VFB37_09185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZO22667.1"
                     /translation="MKIAIATDAWESQTNGVVTTLKATIETLIELGHEIRVISPQGRF
                     SIPAPSYPEIRLALAPGRHIAREIAGFRPHAIHIATEGPVGLAMRRYCRRHRLPFTTS
                     YHTRYPEYLNARWPIPISVSYAWLRRFHNAAARTFVASESLRVQLSAKGFARLHLWRR
                     GVNLERFRPLASSLPQELMGLRRPLMAYMGRLAVEKNIEAFLRLQVPGTKLLIGNGPQ
                     RAELIARYPDAVLTGYRFGDELAALLSSADVFVFPSRTDTFGLAMIEALACGTPVAAF
                     PVPGPLDVLEHGVTGVMSENLAQAIEGALQLDRQVCAERARAFTWIAATRQFFEGLEP
                     IPAAARAALPGRGSAMIAPNAARSQPSSAGDAN"
     gene            complement(3016..3819)
                     /locus_tag="VFB37_09190"
     CDS             complement(3016..3819)
                     /locus_tag="VFB37_09190"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZO22668.1"
                     /translation="MTGAGRGPTRVRTLFLSDIHLGYRRSRVGELAEFLHSVTAQQIV
                     LVGDIIDALSLGRRLFWSAEHTNIVRVLLARRRAGARLLFIPGNHDASLALFAEVLNG
                     QLEVHREWVHRTVRGRRLLVTHGDRFDGIVPCVPWLTRLADALYYVTVAFSHAVNNAR
                     RALGRPYWPVAERIKLGMGPSVRYIESFEQVAAKHARAEGYDGIVCGHIHRANLRDIG
                     GTTYCNTGDWVESCSALIETLDGELQLVRWPDGLSAAHRAMPPLVADAA"
     gene            complement(3937..5259)
                     /gene="phoR"
                     /locus_tag="VFB37_09195"
     CDS             complement(3937..5259)
                     /gene="phoR"
                     /locus_tag="VFB37_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HZO22669.1"
                     /translation="MQPATQAWWFAAGRMIATVLIGLLLGWVFRNVWVGLGSALALHL
                     GWQLANLFRLEWWLRHRSFADPPDVGGVFGEIIAQIVRLHRRKRFHKQRFVQLMRQLQ
                     RSTAALPNGVVILNAQREIIWFNRTAARLLDLRRTGDLGLRIENLLRQPQFARYLAIG
                     DFSNPVVIRTTTGEDCYLSLQVVPYGEGQQLLLVSDVSRQMRLEAMRKDFVANASHEL
                     RSPLTVISGYLETLSQDPALDADLLGPVAEMRRQAERMTSIIRDLLALSRLEETDERI
                     GGEPLDVAALLSILRKDVLARPIHPRDVRVRVESDAQLVGDEPEIHSAFSNIVDNAAK
                     YTPADGSIEMRWWVDEDGGHFSVKDTGIGIPPEHIPRLTERFYRVDAGRSRASGGSGL
                     GLAIVKHVLQRHGATLDVHSTHGAGSEFICHFPLSRLQPVQEIHSAAV"
     gene            complement(5308..6003)
                     /gene="phoB"
                     /locus_tag="VFB37_09200"
     CDS             complement(5308..6003)
                     /gene="phoB"
                     /locus_tag="VFB37_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HZO22670.1"
                     /translation="MPAKQVLIVEDERPIREMIAFGLKRAGFEVREAEDCRAARAALA
                     DLRPDLILVDWMLPDMSGLELTRALKRDRETRELPVIMLTARAEEGDKVSGLEGGADD
                     YVTKPFSPRELLARINAVLRRTTGNGADELIEFEGLALDQASHRVMVEDRTVPLGPTE
                     YRMLEFFMTHPERVYTRDQLLDRIWGGNVYVEERTIDVHIRRLRKALEEFGYDRFVQT
                     VRGSGYRFSARTL"
BASE COUNT         1098 a         2008 c         1958 g          997 t
ORIGIN      
        1 ggtcggccgg cggggatcgt tcggcgcccg gctcaccgta catggcgtgc agggccacat
       61 cgcctacccg cagctcgcgg agaatccggt gcacagcctg gctccggcgc tcgcggagct
      121 gaccagccgg gtctgggatg aaggcaccga gcatttccag ccgaccagct tccaggtctc
      181 gaacctgaat gctggcacag gggcgccgaa cgtgatcccg ggggagctga aggcgcgctt
      241 caacctgcgc tattcaccgg cccagacccc ggaacgcttg cgggagacgg ttgaaggaat
      301 cctgcgcaag cacgccgtgc gctacacgat cgaatggcgc gtatcgggcg agccgttcta
      361 caccgcgccc ggcaccctgt cggatgcggt tggcgcggcc gtcgagagag tcacgggcac
      421 gaggcctaga ttatcgaccg ccgggggtac ctcggacggc cgcttcatcg ccccgttggg
      481 agcccaggtc gtcgagttgg gcgtcgtgaa tgcctcgatc cacaaaatcg acgagcacgt
      541 gcggatcgag gagatcgcgc tgctgcacag gatgtacctc accctgctgc agcacctcct
      601 cgggtagtca gcgggccttg ggccgccgtg gccagaggtt cgtggtgatg ccgacggccc
      661 cgagcagcag tgcgcaaatc aggacccagg cgggcatcgc caggccgaga aacttccagt
      721 tgatctggct gcactcgcca cttccggtca gcaccttgcg gatgacctgg gtgacgggag
      781 tgaactgcag caggacatcg agcggtgcgc cgcaggacgg gatcgtaccg ggcggcagac
      841 tctgaacata caggtgacgc ccggcgaccc ccgcggccgc cagggcggtg gcagcgatca
      901 gcaccgcgta gaggatcgag ccccagccac gcgggttatg caccccggcg aatagaaaaa
      961 cgacccccag ggcggcgatc ccgatccgct ggaagatgca caggggacac ggctcgatgc
     1021 cctggtggta ctggacgaag taggcgtacg cgagcatcgc cacacaggca atgaaccccg
     1081 cgaaattcaa caggcgccga ttagcatgag ccatgaagag gctcagtgta cccgacgggg
     1141 cgctcgcgcg cgtcgatcga actgagcccg gcgggcagcc ctcaggtggc ggttccgggc
     1201 ctgtgctcgc gaagctcgcg ctcgacgtca tcgagcgagg tgtgccggat atccttcccg
     1261 tgcaccatgt agatcacgta ctcgctgatg tttttggcgt gatcgccaat tcgctccaga
     1321 gcgcgaacta cccacatcac atccagcgcg cgccggatcg tgcgcggatc ttccatcatg
     1381 aaggtgatcg cctgccgctg gatggcctcg tattcctcat ccacgagccg gtcctggcgg
     1441 gccacccgca acgccgactc gacatccatg cgggcgaaag catcgagtgc gtcatgcacc
     1501 atatccgaca ccacccgacc cagatgcttg atctcccggt acttgtccgc cggccgctcc
     1561 atcgtcgcca ggcgcgaggc gatgtagccg atcttctccc cctcgtcccc gatgcgctcc
     1621 aggtccgtga tggtcttgat gatggcgacg atcacacgca agtctcccgc cgcgggagcg
     1681 cgcgtggcga ggatacggct gcactcctcg tcgatggcca cctccatgtt gttgacctgg
     1741 tagtcatcca tcgccaccga ttcgcccagc gtactgtcgc cttcgacgag tgcggcgacg
     1801 gccttgtgga gctgttgttc gacgaagccg cccatcccga gcaccttgct gcgaacgcgc
     1861 tcgagatcct cgttgaagcg gcgcgaaatg tggtggctga ggtcggcggt ttccatcagt
     1921 tggcgtctcc ggcgctgctc ggctgactac gcgccgcgtt gggcgcgatc atagccgaac
     1981 cgcggcccgg cagtgccgct cgtgcggcgg ccggtatggg ctccagaccc tcgaagaact
     2041 ggcgggtggc ggcgatccag gtgaatgccc gggcccgctc ggcgcatacc tgacgatcca
     2101 gctgcagggc accttcgatc gcttgcgcga gattctcgct catgacgccc gtgaccccgt
     2161 gctcgagcac atcgagaggg ccgggcaccg ggaatgccgc caccggcgtg ccgcaggcga
     2221 gcgcctcgat catggccaga ccgaaagtat cggtgcggct cggaaagacg aagacatcgg
     2281 cgctgctgag cagagcggcc agctcatcgc cgaaacgata gccagtgagc acggcgtccg
     2341 gataccgggc gatcagctca gcgcgttgcg gcccgttgcc gatcaacagc ttggtccccg
     2401 gcacctgcag gcgcaggaac gcctctatgt tcttctcgac tgcgagacgg cccatgtacg
     2461 ccatgagcgg tcggcgcagc cccatcaact cctgcggcag gctcgaagcc aggggccgga
     2521 atcgctcgag attgactccg cgccgccaca agtgcagccg tgcgaaaccc tttgcggaca
     2581 gctgcacacg caggctctca ctggcgacaa aagtccgtgc cgccgcgttg tgaaatcgcc
     2641 tcaaccacgc atagctcacc gagattggaa ttggccagcg cgcgttcaga tactccgggt
     2701 agcgagtgtg gtaggacgtc gtgaacggca gccgatggcg gcggcagtaa cgccgcatgg
     2761 cgaggccgac cggtccttcc gtcgcgatat gaatggcatg cggacgaaag ccggcgatct
     2821 cgcgggcgat gtgccggcct ggagcgaggg cgagacggat ctcggggtaa ctcggagccg
     2881 gaatgctgaa tcgtccttgc ggcgagatca cgcggatctc gtgacccagc tcaatcagag
     2941 tctcgatcgt ggccttcagc gtcgtgacga cgccgttggt ctgggactcc cacgcatcgg
     3001 tggcgattgc gatcttcaag cggcatccgc caccagcggc ggcatcgccc gatgcgccgc
     3061 agacagacca tctggccagc gcaccagctg tagctcgcca tccagggttt cgatgagtgc
     3121 tgaacaggat tcgacccaat cgcccgtgtt gcagtaagtc gttccgccga tgtcccgcag
     3181 gttggcccgg tgaatgtgcc cgcagacgat gccgtcgtag ccttctgcgc gcgcgtgctt
     3241 cgccgcgacc tgctcgaagg actcgatgta ccggacgctc ggacccatgc cgagcttgat
     3301 tcgctccgcc acgggccagt acggccggcc gagcgcgcgg cgcgcattgt tgacggcgtg
     3361 gctgaaagcg accgtcacgt aatagagcgc atctgcgagc cgcgtgagcc aaggcacgca
     3421 aggaacgatc ccatcaaagc gatcgccgtg cgtgacgagc aacctcctgc cccgcacggt
     3481 acggtgcacc cattcccggt ggacctcgag ctgaccattc agaacctcgg caaacagcgc
     3541 caggctcgca tcatgatttc ccgggatgaa cagcagccgc gcgccggcgc gccggcgcgc
     3601 gagcaggacg cggacgatgt tcgtgtgctc ggcgctccag aagagccgcc ggccgagact
     3661 cagagcatcg atgatgtccc cgaccagcac gatctgctga gccgtgacac tgtggaggaa
     3721 ctcagccagc tcgcccacgc gcgagcggcg atatccgagg tggatgtcgg acaggaacag
     3781 cgtgcggacc cgggtggggc ctcggcctgc gccagtcatc atgctgtccc cgcgaaaggc
     3841 gattgctgca cgccggctag gctcgcaggc cagtatgtcg ggcagatgac agagccgcga
     3901 aatatttctg acggatgagg cacttgcagc ggaaagctag acagcggcgc tgtggatctc
     3961 ctgcaccggt tgcagccgcg agagcgggaa gtggcaaatg aactcgctcc cggcgccgtg
     4021 cgtgctatgc acatccagcg tggcaccgtg ccgctgcagc acgtgcttca cgatcgccag
     4081 ccctagacct gagccgccgc tcgcgcgtga gcggccggca tcgactcgat agaagcgctc
     4141 cgtcaaacgg gggatgtgtt cgggcggaat gccgatgccg gtgtccttca ctgaaaagtg
     4201 tcccccgtcc tcatccaccc accagcgcat ctcgatcgag ccgtccgccg gggtgtactt
     4261 cgcggcgttg tccacgatgt tggaaaaggc cgaatgaatc tcgggctcat ctccgaccag
     4321 ctgcgcgtcc gactccactc ggacccggac gtctcgcgga tggatcgggc gggcgagtac
     4381 gtccttgcgc aagatggaga ggagcgcggc gacgtccagc ggctctccgc cgattctctc
     4441 gtcggtctcc tccagccgcg aaagtgccag gaggtcgcgg atgatgctcg tcatgcgctc
     4501 ggcctggcgg cgcatctcgg ccaccggccc cagcagatcc gcatccagag ccgggtcctg
     4561 gctcagggtt tccaggtatc ccgagatgac cgtcagtggc gagcgcagct catgcgaggc
     4621 gttggcgacg aagtccttgc gcatggcctc gagccgcatc tgccgcgaca catcgctcac
     4681 cagcagcagc tgctgccctt ccccgtaggg aacaacctgc agcgagaggt aacagtcttc
     4741 ccccgtcgtc gtgcggatca ccaccggatt ggagaagtcg ccgatcgcga ggtagcgcgc
     4801 gaactgcggc tggcgcagca gattttcgat ccgcagcccg agatctcccg tccggcgcag
     4861 atcgagcagc cgggcggcgg tacgattgaa ccagatgatc tcgcgctgcg cgttcagaat
     4921 gaccacgccg ttcggcagcg cggcggtgga gcgctgcagc tggcgcatga gctgcacgaa
     4981 gcgctgcttg tggaagcgct tgcgccgatg caggcgcacg atctgcgcga tgatctcgcc
     5041 gaacaccccg ccgacgtccg gcgggtccgc aaagctgcgg tgtcgcagcc accactcgag
     5101 ccggaacagg ttggcgagct gccatcccag gtgcagcgcc agggcgctgc cgagcccgac
     5161 ccagacgttg cggaacaccc agccgaggag caagccgatc agcacggtcg cgatcatgcg
     5221 acccgccgcg aaccaccatg cctgtgtcgc cggctgcatg cgcgtcctca aattccactg
     5281 acctgcgcag gacccacccg ggccccgcta cagggtacga gccgagaatc gatagcccga
     5341 gccgcggacg gtctgcacga accgatcgta tccgaactcc tccagagctt tgcgaagcct
     5401 gcggatatgc acgtcgatcg tacgttcctc gacgtagaca tttccacccc agatccgatc
     5461 gagcagctgg tcgcgggtat agacccgctc ggggtgcgtc atgaagaact cgagcatgcg
     5521 atattcggtc ggtccgagag gcacggtccg gtcctcgacc atcacccgat ggctggcctg
     5581 atcgagcgcg agtccctcga attcgatcag ctcatcggcc ccgttgccgg tcgtccggcg
     5641 cagcaccgca ttgatgcgcg ccagcagctc gcgtggggaa aaaggcttcg tcacatagtc
     5701 atcggcgccc ccttccagtc cggagacctt gtcgccctct tccgcccggg cggtgagcat
     5761 gatgaccggc agctcgcgcg tctcgcgatc ccgcttcagg gcgcgggtga gctccagtcc
     5821 actcatgtcg gggagcatcc aatcgaccag gatcagatcc gggcgcagat cggccagggc
     5881 ggcgcgtgcg gcgcggcaat cctccgcctc acgcacctcg aagccggcac gcttcagccc
     5941 gaaggcgatc atttcgcgaa tcgggcgctc gtcctcgacg atcagaacct gtttggcggg
     6001 catatgtgac agatttgggg ccgctgctat tacagaggcc gcatgttgca gattcatgac
     6061 a
//
LOCUS       DASRHN010000238        82081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1724797, whole genome shotgun sequence.
ACCESSION   DASRHN010000238 DASRHN010000000
VERSION     DASRHN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 82081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724797"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFB37_09205"
     CDS             <1..215
                     /locus_tag="VFB37_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO22671.1"
                     /translation="AVLLSAGNRVLSRAQLTELLRGREADPFDRSIDVRVSRLRQILG
                     DDARSPQIIKTVYGEGYVIGVPVEST"
     gene            234..1832
                     /locus_tag="VFB37_09210"
     CDS             234..1832
                     /locus_tag="VFB37_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22672.1"
                     /translation="MRIPLWPQSLFGRLIAALVIAVVLAQAASLYLFAHDRERFVLDS
                     SVREWSHRITEITLLLEQLPTGQRAETIARLKERGGRLAPRHGLRMSGPRDATWSDGP
                     LLMPPSHSSPAFGAAPRAGESNADIPATQERAYSGHHPMLPPHAHRLMRVLHRQREET
                     FWPRIFIQLPFASNAQEVLAEQLRAQLGKDYEVSVQPAAAPAASVIPIPSPFFGMPAQ
                     RPTESYDVDVRLPDSSLLVYRVTRVSPGAPLPPSLMVNLILLVAVLVVALYVTARSIT
                     RPLSRLALAADNLGRSVRQPKLEEQGARELRRAARAFNTMQDRLHRYLDSRTRVLAAM
                     SHDLKTPLTRLRLQVETQIEDPALQARFGKELDEMEGMVRGALALFRGLNDEEALEPV
                     DINLLLETVRGEFTEMGKDVTVEGHALGPLPGKPQALKRCITNLVGNAVNFGGRARIL
                     VHDGTLLTISVCDEGPGIPPEELEKVFEPFYRLESSRNRDTGGTGLGLSIARDVAQAH
                     GGSLVLENRPGGGLQARLSLPRGR"
     gene            1965..2450
                     /locus_tag="VFB37_09215"
     CDS             1965..2450
                     /locus_tag="VFB37_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22673.1"
                     /translation="MISRSVVMMAMLAVAGTALAQAPPSPSPQWQGHHQWKSSAEWRQ
                     KMEQRRMQRLTVLLDLTPAQQQQVRTIFSDEHAKMKAAMEPMREAMKQMRAAHEAAHK
                     DALQRLGSVLSPAQMKKLDALMPPRGMMRGHWMMMHGMGHGEKMGPPPGSDEGAGSNP
                     P"
     gene            complement(2455..3195)
                     /locus_tag="VFB37_09220"
     CDS             complement(2455..3195)
                     /locus_tag="VFB37_09220"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZO22674.1"
                     /translation="MPTRSLFVSDVHLDPAAPEAQRQFLEFLQTHVGGSTARTHALYI
                     LGDLFESWVGDDDLSSDRAEVLAALRALTTGGIACFLLHGNRDFLIGQDFSLQTGCQL
                     LPDPVIAEIAGEPVLLTHGDALCVDDHPYQELRSIVRAPVWQRRFLRLPLSDRQLLAD
                     GVRAGSRAHTSRTIPKIMDVNPAAVTAAFKAARVRRIIHGHTHRPAIHDTLMEGQPVQ
                     RIVLGAWYEQGSYLECEAGRYELRTLPR"
     gene            complement(3237..4328)
                     /locus_tag="VFB37_09225"
     CDS             complement(3237..4328)
                     /locus_tag="VFB37_09225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO22675.1"
                     /translation="MHDMVRGLPRSRHTASSRAPGTAKIPVSTTYCDGDPMRARYRTL
                     MMKPFSGGLAITRAGLGGLLVLAAALACLLLIRPAAAADAPPAPNPQVRVTTNMGSFV
                     IELRPDRAPLTVADFLHYVRDGFYSNTLFHRVVANFVVQGGGHAATDPYALKPTYPPV
                     DNESGNGLQNKRGSVGLARAAGPHTGNAQFYINLVDNPELDPLPTRWGYAVFGQVVEG
                     MDVIDRIGVVPTGTFGPFKSDAPLKPVIIQSVELVGAAQTTTPAPSPQPGAQQSGPPP
                     AGTPPTQTPPSGSAQPGTPQPGSPPSSQPPASSPQPQPGQPGAPASTSPQPATPPPGT
                     PPAPAGDKSQGTKPPPQTPPGEADQPPPI"
     gene            4336..5763
                     /gene="gltX"
                     /locus_tag="VFB37_09230"
     CDS             4336..5763
                     /gene="gltX"
                     /locus_tag="VFB37_09230"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZO22676.1"
                     /translation="MDSTAPTGFDGGAVTRFAPSPTGELHLGNARTALFNFLLAHRSG
                     GRFLLRIEDTDAQRSQEAHLAVVLEDLRWLGLDWDAGPDKEDDGGPYRQSQRGALYAR
                     YFRALEQRNAVYPCFCTPLELEAARRAQLASGRPPRYAGTCRELSAEQRRNKEAQGLA
                     ASLRFRVPAGRRIEFVDLVHGAQSFLSDDIGDFVIRRAEGTASFFFSNAVDDACMGVT
                     QVLRGEDHLANTPRQLLILEALELRAPGYGHVSLILGPDGAPLSKRHGATSVREYRES
                     GYRPEALRNHLFRLGHSSGEHDLLDLPAMARAFDPGHLGRASAHFDAQQLHVWQKEAA
                     HRLSVDDALVWLAPVLPAGLSEPAARAFVAAILPNVVLPQDAQPWARIVFGPPPELED
                     DAGEIVSKAGERYFAAAAQAAAQSGNDLPAIVSAVRAATGKKGAELYMPLRAALTGRS
                     HGPELAPLLKAMPAGTAQGRLARFA"
     gene            5787..7211
                     /gene="cysS"
                     /locus_tag="VFB37_09235"
     CDS             5787..7211
                     /gene="cysS"
                     /locus_tag="VFB37_09235"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZO22677.1"
                     /translation="MIRIHNSLTGEKQPLAPVTPGEVRMYVCGLTVYDFMHIGHARMM
                     IVFDVVNRYLRHRGLRVTYVRNITDIDDKIIRRAAENGEPIGALTERFIAAMHQDCDE
                     LGVARPDHEPRATQYVPAIIEMVEQLVARDYAYVAPNGDVMYAVAKFDGYGRLSGKRL
                     ADLRAGARVEIDEAKRDPLDFVLWKHAKPGEPAWDSPWGKGRPGWHIECSAMSVALLG
                     SHFDIHGGGLDLKFPHHENEIAQTCAATGDRFAEIWMHNGFVNIDNEKMSKSLGNFFT
                     VREVLDKAVRHPQVLRYFVLSSHYRGPINYSLEQLEQADAALGRIMTALRDLPQSSAP
                     TSEYLGPFREAMDDDFNTPEAVAVLQTLTREINTAKSAGNEMRAAALGAELRSLAGVL
                     GITVTPKEWFSLGAKLVSQGGDRVAAGATTLADAEIAQRIEARIAARKAKNWAESDRI
                     REELAAAGVILEDKPGGKTEWRRA"
     gene            complement(7389..8252)
                     /gene="folD"
                     /locus_tag="VFB37_09240"
     CDS             complement(7389..8252)
                     /gene="folD"
                     /locus_tag="VFB37_09240"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZO22678.1"
                     /translation="MTAQLIDGRELAAEVKNQVRGRIEAALARGSRRPALAVVKVGDD
                     PASEVYVRNKRKACEEVGILSVAHDLSDSTSQAALLALVDDLNRDASVDGILVQLPLP
                     DQIQQTAVIEKIDPAKDVDGFHPYNVGRLAQRIPLMRPCTPYGVIRLLERVGAPFKGQ
                     HAVVVGASNIVGRPMSLELLLMGATTTVCHRFTTGLDTYVLQADILVVAVGKPGLIPG
                     EWIKPGAVVIDVGMNRLENGHLVGDVQFESARRRAGWITPVPGGVGPMTVAMLMHNTL
                     EAYHRRQESGR"
     gene            8331..8407
                     /locus_tag="VFB37_09245"
     tRNA            8331..8407
                     /locus_tag="VFB37_09245"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8365..8367,aa:Pro,seq:tgg)
     gene            8893..9360
                     /locus_tag="VFB37_09250"
     CDS             8893..9360
                     /locus_tag="VFB37_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO22679.1"
                     /translation="MASIYDSENYEPCRSVAHLVGQVRTELLAALDRELTADKHVADI
                     GVTSAQFIVMARLAAAERKKSASDLCKEMSYDAGAMTRMIDRLESKGLIRRARCPQDR
                     RLVYLEMTDQGRAAYPRLREISMAIQNRFLRGFSRTDTRQLESLLGRMLENAA"
     gene            9446..10717
                     /locus_tag="VFB37_09255"
     CDS             9446..10717
                     /locus_tag="VFB37_09255"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZO22680.1"
                     /translation="MTTREETAKTSAWKSPRVRWLVLVLGGFLVLGAAWGVYWDRDLR
                     YSVYTDDAYVSGNVVQITPQIAGTVVAIGADNTQFVKAGQPLVRLDPADAKVALQQSE
                     AGLAKTVRQVRNLFATTSELQANVEVRRTDLAKAQSDYDRRARLERSGAISLEELQHA
                     GDAVKSAQAALLAAQQQMAADQALIDGTTIQSNPAVRDAAAAVHAAYLNYARTVLPAP
                     VSGFVARRDVQLGERVNTGTPLMAVVPLNEVWVDANFKESQLQSMRVGQPVSLTSDLY
                     GSGVVFHGHVVGFGAGTGSAFSLLPAQNATGNWIKIVQRVPVRVALDPQELAQHPLQI
                     GLSMKAYVNVRDDNAQRLPQVAANDRDYSTDVFQSVAAQADATVQQIIAANSPLAGRS
                     STGHRFAARLNRGSTLLAVPARATLPVALSE"
     gene            10760..12376
                     /locus_tag="VFB37_09260"
     CDS             10760..12376
                     /locus_tag="VFB37_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO22681.1"
                     /translation="MSAVIREQQDVPPQSRPAAAAPAPKSDMPCLKGSALALGTIALS
                     LATFMNVLDTSIANVAIPSIAGDLGVSPDQGTWVITSFAVANAISMPLTGWLTRRIGQ
                     VRLFTASTLLFVVASFLCGLAPSIGMLVAFRALQGLVAGPMIPLSQSLLLASYPKAKA
                     GAALTVWSMTTLVAPVVGPVLGGWITDNISWPWIFYINVPIGIAAVVVTWAIYRRRET
                     PTARLPIDKVGLTLLIVWVGAMQILLDKGKNLDWFNSPFIVTLAIIAVVGFAVFMIWE
                     LTDKHPIVDLSLFRGRNFSVSTVAMLVGYGLYFGNVVLLPLWLQQYMGYTATLAGVVL
                     APVGVLAILLMPVVGRFLSRIDPRWIVTVSFLVFGLVMFQRSWFNTQADIRTLLVPTL
                     IQGVALATFFVPLLTLGFSGLSADRIPAASGLMNFARITAGSFATSITTTLWDRRATL
                     HHAQLVEHLIGSNASMTQALGQLHADGLTSQQGYALLNNLVDNQAYMLSANDIFYVSG
                     FLFVVLAGLVWLARPSKAGGAGKEAAAGAH"
     gene            complement(12373..13806)
                     /locus_tag="VFB37_09265"
     CDS             complement(12373..13806)
                     /locus_tag="VFB37_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22682.1"
                     /translation="MNTSPSEHGADHDVQALRSTVQLLQDELTQSNQEIAALALELEQ
                     GIDELKRSNQALLAEIEERRKAERERQHLHEALRLAYEELRQSQERLVQQERLRALGQ
                     MASGIAHDINNAISPIALYTASLLEHDSTLSQRAREYMTIIQRAIEDVAATVARMREF
                     YRPRESTLQLAPVELNEVVEQVVALTRARWSDLPQQRGVVVHVRTELTSKLPAIMGVG
                     SEIRDALTNLVFNAVDAMPQGGTLTLRTAARANSVSRSDSTAAEHVLVEVRDTGNGMD
                     EVTRRRCLEPFYTTKGERGTGLGLAMVYGMARRHSAELEIESTPNAGTAVRLIFPLSS
                     PAPDGEHEQRVADAPARAMRLLLVDDDPLLIRSLRDALEADGHEVVTADGGQRGIDTF
                     TAALRAGRPFDLVITDLGMPYVDGRKVSAAIKALAPPTPIVLLTGWGRHMPLEGEPAP
                     QVDYILSKPPKLAELRQTLAECASKRG"
     gene            complement(13803..14834)
                     /locus_tag="VFB37_09270"
     CDS             complement(13803..14834)
                     /locus_tag="VFB37_09270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22683.1"
                     /translation="MSQPGASSPRPVTMRIADPIDIRIAEDAARRLSASVGFSAGDCE
                     EVALAVTELASNLIRHAGGGTLSLEPASREARLGIQIESADSGPGFDDFERALTDGFS
                     TSRSLGTGLGAVNRLMDELEYSPAGSAGAHIVCRRWLRQRAATDAAPALDFGVASRAY
                     RHLHENGDAFVIRKWGDKALAGVIDGLGHGQFAQRAAQAARQYVEQHHDQPLEALFRG
                     AGRACQATRGVVMMLVLLDASRRTFTVASVGDIELRVWGGPERLNLIPRRGVVGLRAP
                     APAIAEHSFAPGTILVMHSDGLRSRWDFSDFPWIAQETATVAAQRLLVALARDDDDAT
                     VLVVRTAPS"
     gene            complement(14831..15241)
                     /locus_tag="VFB37_09275"
     CDS             complement(14831..15241)
                     /locus_tag="VFB37_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HZO22684.1"
                     /translation="MTGTAQGEVSIRSEGDIVAARRSVREAVMELGFGLTDVTRVVTA
                     ASELARNVHKYGGGGVMRWRRLESGGRRGIELEFIDRGPGIADVELAMQQGYSSGKGL
                     GLGLPAARRLMDELEIRTAPGAGTTVTSRKWCPP"
     gene            complement(15238..15618)
                     /locus_tag="VFB37_09280"
     CDS             complement(15238..15618)
                     /locus_tag="VFB37_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZO22685.1"
                     /translation="MSDRNSISIIIVRKTLMVTVPSEPDDATVSALQDEVLAAMQRVD
                     AKGVVLDISSVETLDSFFARTVAETASMVSLMGGRTVVVGMRPSVAVTATQIGITLGG
                     IETALSVERALDLLDRPVAGRSRR"
     gene            complement(15654..16520)
                     /locus_tag="VFB37_09285"
     CDS             complement(15654..16520)
                     /locus_tag="VFB37_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO22686.1"
                     /translation="MGTQFESRPGGHPGAAASQTSPETQELVRLLIDNIRDYAIITLD
                     PRGHVLSWNAAAERLKGWKADEIIGQHFSRFYPPEDLQNGKTEMELKVASQEGRFEDE
                     GWRVRKDGSRFWANVIITALRDAEGRLRGFGKVTRDLTARRESEEKIRRQAQEILEMA
                     AVPVVQVWAGILLVPLIGTLNSQRTQQLMDVLLNKVMETASPVALLDITGVPAIDTQT
                     AQHLIETIAAVRLLGSEVVLTGVRPSIAQTLVHLGIDLSNVSTRSSLSAGLRTAFELL
                     ELCVAPRAGAGG"
     gene            complement(16670..17170)
                     /gene="sixA"
                     /locus_tag="VFB37_09290"
     CDS             complement(16670..17170)
                     /gene="sixA"
                     /locus_tag="VFB37_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZO22687.1"
                     /translation="MQLLIVRHAIAFERDAKRWPDDNERPLTVEGIKKARRAAAGLRR
                     IAQRPARVLTSPLVRARDTAAILTQAARWPQAVACDALAPGKSPQEVLAALRREGAGA
                     ECVAVVGHQPGLGKLLALCLRGDPRPEGFELKKGAVLCLGFEGPPRAGQGTLTALLPP
                     RALRKA"
     gene            complement(17173..18900)
                     /locus_tag="VFB37_09295"
     CDS             complement(17173..18900)
                     /locus_tag="VFB37_09295"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZO22688.1"
                     /translation="MDATAREEPGKEREIEWQLAATDLGLVRTWLGRHPTLDGLSIKR
                     LPTLHLHDTYLDTPDWRIFRAGFALRLREERGGKVEATLKGLRSARDDLADRRELTEP
                     LPGRSIKALARAEGPVGSRVRDVTGSRPLRTLFEVHTSRRRFAVLSRDPSRQLGEIAL
                     DETQFSRSDSHRSSTRLKRVEVESAGPEHGSLERLANRLRAECGLHPATENKFAAGLR
                     SVGLEPPQGRKPDETAAPAPDAMDVATGAREFAIAALQRLVDEWCAHEPGARLGESPE
                     ALHALRVTGRRMDTVLSLFGAYLPAALRRSRPKLKSLLRALGAVRDVDIRVAVASTFR
                     NSLAETDRRALDPLLGQLADERREAHSTMLRVLDAKPARQWLDALPDQLARAAAQRVA
                     SARTACALAVVPDLLRRRYRKLRKCARQMRPGSPMGEYHKLRIHTKKLRYALEVVAPS
                     YAKPAREMLGALRKLQSRLGIQHDGDAVHRYLTQLAKQPPAGFGSQTLFLIGRLAEPQ
                     SRNAIRMGDKVEKSWRKVRGRRWKTLRSRMQDLRDGTAQIRSKPKGIADGPVASARDG
                     SASADADGS"
     gene            19165..19241
                     /locus_tag="VFB37_09300"
     tRNA            19165..19241
                     /locus_tag="VFB37_09300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19199..19201,aa:Arg,seq:tct)
     gene            19357..19432
                     /locus_tag="VFB37_09305"
     tRNA            19357..19432
                     /locus_tag="VFB37_09305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19390..19392,aa:His,seq:gtg)
     gene            19445..19520
                     /locus_tag="VFB37_09310"
     tRNA            19445..19520
                     /locus_tag="VFB37_09310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19478..19480,aa:Lys,seq:ctt)
     gene            complement(19828..20553)
                     /locus_tag="VFB37_09315"
     CDS             complement(19828..20553)
                     /locus_tag="VFB37_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZO22689.1"
                     /translation="MRTIKSTSTGRSYDLYVRKPVDYDKNKQQKYPVLYLLDGQWDFK
                     LLDSVIGGLVYDKAMPDILVVGIAYSGERPDYNALRAMDYTPVPGDAKGSGGGPKFLS
                     FLKSELIPLIEANYRADPARRILGGDSFGGLFTLYAMFTDSSLFWGYLAGSPDIEFAD
                     HFIVKQEEEFAKTHKDLRVRIFFAAGGDESPLAPRIAFVRTFAGRNYNGLHWDGRVIE
                     GEGHASAKPEFYSLGLRFLFGNG"
     gene            20793..20975
                     /locus_tag="VFB37_09320"
     CDS             20793..20975
                     /locus_tag="VFB37_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22690.1"
                     /translation="MSCALGGVPGARSLFARYLRTNVRTLENWEQGRAKPNAQAALLI
                     RLVQRYPDTVRRLAEI"
     gene            21269..22765
                     /locus_tag="VFB37_09325"
     CDS             21269..22765
                     /locus_tag="VFB37_09325"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZO22691.1"
                     /translation="MRDIQRLNTGIGALDDILHGGLIPERLYLVDGNPGSGKTTLALQ
                     FLLEGARRGEKCLYVTLSETRKELEAGAASHGWSLDAIDIVELIPDQQHELNGEGALT
                     MIQPSDVELSETMRKVLDAIERSNPARLVLDSLSEMRLLAQSSLRYRRQILALKQFFV
                     GRRCTVLMLDDRTAEGPDLQLHSIAHGVMAFYVTTPVYGQARRQLQVLKFRGSDFSSG
                     FHDFDIRHGGLVVFPRLVAADHGTRFDQSVIKSGVVALDALLGEGIERGTSTLLVGPP
                     GSGKSTLALQYACAATQRGDHAASFVFDETKAALLARSQGIGLSLREGAGPGEIALQQ
                     IDPVEISPGEFAAMVRKCVEQDGARVVVIDSLNGYLNAMPQDNFLVAQLHELLSYLSN
                     QGVTTLMVVAQSGMLGTSMTSPVDASYLADSIVMLRYFEHGGRVKKALSVMKKRTGGH
                     EGSIRELWFDKEGVHLSEPILGLRGVLTGVPVELGGGAGGGAERPLRP"
     gene            22774..24300
                     /locus_tag="VFB37_09330"
     CDS             22774..24300
                     /locus_tag="VFB37_09330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZO22692.1"
                     /translation="MNEQGVLIYPPSRRDGQVTREVLRRASLVCTVCHEALEVAHEID
                     AGSGVLVMTDAALALPSVHIILTSLSQQPAWSDLPVVLLCQTDPETPVVAGILGAFTN
                     VTVLDRPTSARTLISTVQAALRGRLRQYQMRDQLVALRVAKETLRNADRRKDEFLAML
                     AHELRNPLAPIRNAGELLARMLPDDSQVQTVAAIVKRQSSHLSRMVDDLLDVSRITQG
                     RINLEHEPISLASVISQALESVEPLMRENRHKVFVAGGYGPAYVLGDHARLVQCVANI
                     LTNASKYTDPGGEIRVEMRSDGSQAVITVTDNGVGIPAELLPQIFDLFVQSNRSLDRS
                     QGGLGIGLSVVRRLVEMHGGRVSAASDGPGRGARFEITLPLIDRPSEVREEQSEQTAA
                     RRRILIVDDNADSADSLAMILNLCGHVAEPVYGAAEALERAAALDPEIVLLDIGLPGM
                     DGYDVARLLRSRGSSARLVALTGYGQPEDVRRAQDAGFDTHLVKPVDLKHLLRDLGRA
                     "
     gene            25186..26286
                     /locus_tag="VFB37_09335"
     CDS             25186..26286
                     /locus_tag="VFB37_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZO22693.1"
                     /translation="MTLAPIRPHPIVIWFGRVGDMILLSALLGLLHERYGARCFVVGA
                     GAWISDIYKRHQDVEQVWYLRRYTPFLVDAGWWRVFGALRRHRSEPIYICETDARKLK
                     RIRWLLALSRTSPDRCVFMNEELLAAESRGQPLEHWVDRLLSLGQRTPPALSQTDFQV
                     PAGGARAPRLDVSAEDRARTEEWLRAKGWYGRPLVLIQPGNRRTMRGKKLKRSAADDK
                     AWPIETWAALLRRIHTALPNAVIVLVGAPKEALLLGWIEEAAALHSVATATLPLADLF
                     ALCAIAHSMISVDSGPAHAAAAVGLPLVVLFGAHSQREWLPRSAGSPVVGIGGPPVSR
                     RLDQISDETVFEAWLTLSGAQAGLGSRVGRRT"
     gene            26356..27801
                     /locus_tag="VFB37_09340"
     CDS             26356..27801
                     /locus_tag="VFB37_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZO22694.1"
                     /translation="MPPAQDGLEEDRAKGSAAGPDGGSRERLRARAKRRLLGTHSKRL
                     FLQVGLPWGAALLVGLVSVLYADWSTAASNAFLRLIAGRPWLAFVITPALTVLALFLT
                     RRWFTGSEGSGIPQVIAALHAPRTEEEDTLLRRLFGLRVIVGKIGVSLIGLLGGLTIG
                     REGPTVHIGAAIMAETRRFYPHRNAQLERRLLLAGAAAGLSAAFNTPLAGIIFAIEEL
                     ARDFESRTNGIMITAVVFSGLTSLALAGNYLYFGQINVPENLGGAFVLPVVLSAALCG
                     LFGTAFNLAVLRWKSWMPEWLVALRARWPLLYALLIGLLVAALGVGTHGETWGSGYDQ
                     ARHLLTGTVPLSEAYGLTKWGAMVVTYMAGIPGGLFSPTLSIGAGIAQWVHAAFSWAP
                     MAAVIALCMTGYLAAVTQSPITSFVIVMEMTNGTGMVIPMMATALVASRISAFFTPPL
                     YEALAASNYFHVAALPTGSFLQKKPVPPPGA"
     gene            27813..28112
                     /locus_tag="VFB37_09345"
     CDS             27813..28112
                     /locus_tag="VFB37_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22695.1"
                     /translation="MKSTLLIAAGTVALLAAASTVRVIRTPTLAAVLQLSGSVLLVIM
                     VLTHIAEAYALAPQMGWGRPNTPGHYLDLVSAVAGLTLLLAGISLRAIGTGRRAS"
     gene            complement(28122..29459)
                     /locus_tag="VFB37_09350"
     CDS             complement(28122..29459)
                     /locus_tag="VFB37_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZO22696.1"
                     /translation="MTDKPTGLKRALNRWDLILYGVIVIQLTAPMPSFGVLSARANGH
                     VATTILLAMIAMAFTAISYGRMAAAHPSAGSAFTYVTREFTPVLGYVTGWAMLMDYVL
                     NPLISIIWCSRAAMNFAPDVPYAVWAIAFFALFTSVNLVGIKSSARISGALAATTGGV
                     VLVFLVCAGLYVRHHNPGSPGFFTHPFYDPKTFRAGAVLNGTSIAVLTYIGFDGISTL
                     SEEVEDPRRNILFATVGTCLVIGILSAIEVYAAQLVWPTALPFRNVDTAFVEVAARAA
                     GPWLFVVMNLALLIAGIGSGVGSHLGAARLLYGMGRSNALPPEFFGAVDPKHRIPRNN
                     VILVGLIALCGAFLISYGLGAEILNFGALFAYMGVNAAALARYYVRSRERRLRDFWPP
                     AAGFVTCLILWIYLSNAAKLVGASWMIVGVIVGAVRTRGFRRDVLRFEVSPED"
     gene            complement(29503..30456)
                     /locus_tag="VFB37_09355"
     CDS             complement(29503..30456)
                     /locus_tag="VFB37_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO22697.1"
                     /translation="MTRESTHCFAADLGGTKLAAAMADAHGRIVAELTEPTDRRGGAH
                     VAEQIAASALKLAHAVGIEAARVRHVMVGVPGAIDPLTGRVSLTPNIEGLQDFDVLDY
                     LRGRFGPGVEIENDVNLAMLGEQTLGCASRCRNAAFLALGTGAGLGLLIDGKLFRGAR
                     GSAGEIADLPIGRDPTSQAAATSGAFELEVGSLAIVERYRRQGGAAAVTVRDIFRLAQ
                     EGDEVAAVVLDTTARWVALAVATLQSLLDLELIVFGGSIGVRPELYERVRLALPAHFS
                     RPITIAPSRLGDRAGLIGAVCAAARALRQDREPAQHALHLT"
     gene            complement(30466..31650)
                     /locus_tag="VFB37_09360"
     CDS             complement(30466..31650)
                     /locus_tag="VFB37_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HZO22698.1"
                     /translation="MASVTRQLSRRSVFEALLHRSPISRADLAKVTGLSKQTTSEVID
                     AFEQQGLVRPIGRTSGNVGRTAVLYELSPDGGHVLGIDLGARLTVTLGDIAGRVLAET
                     DEPTDSRGGAWALEQIARLADRLARDNHTHPSRIRSIVLATPGVVNPRSGAIEMAPNI
                     TGLGHLNVVGLLSEKLGSPVAIENDINLALLGEIWHGCAQNVANVAFLALGTGVGLGL
                     YVNGQLVRGENGAAGEIGYLPIGGDPLQAEARLQGCLEYQVGAHGIVRRYREAGGGDV
                     GSAREVFERAQSGDPRATDVIDETARLTGLAAATVIATVDPQLIVLGGSVGARADFAD
                     KVARVLLRLAPRPVEIRTSTLGKRAGVVGALSVALNKLHDELFSVADLAAALPLPPPR
                     VQ"
     gene            32043..34280
                     /locus_tag="VFB37_09365"
     CDS             32043..34280
                     /locus_tag="VFB37_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HZO22699.1"
                     /translation="MPQSAQARLTGSGVVKVADGALVAVHGVDRQEVEPVADRFVKLV
                     AVTRGLKLRAAAAADAHPAISFEVDPHASVVGDAGYRIVVSAGGVRVIARSARGAFYG
                     SVTLWQLLTPPGWIRGTPAEIPEGRIDDHPRFAWRALLLDSGRHVQSVSDIRKLIDWM
                     ALEKLNVLLWHLTEDQGWRLEIPKYPALTKTGACRKAVGLDIELTGGADKPYCGFYTE
                     AQVRDIVRYAAAHFITVVPGIDLPGHSQAAVAVYPWLGVTGKRPSVWTSWGVSPWLLK
                     PDERTLQFVDDVLDEVMRLFPSQYVSIGGDEADKQQWNASPEVRAQMRQFGLANMDQL
                     QGWFTGRVADYLVKHGRTPLGWDDELLAGATLPASEVVMSWHGTDNERVALEAIRQGH
                     DVVMTPEESLYFDHYQSELPDEWPGQPPAITLRQAYDTAVVPDGATAAEARRVIGVQA
                     CLWTEFMPSFVRDQHALFPRIAALSELGWSPADAHDWNGFLQRLTAELARYRALGIGY
                     SDTPFAAASVVTAGRGGTLHVVLSNQTSFGKIAYTTDGSAPTSASAQYVHPLEFSSRA
                     KVTLRAATFAPDGFDLAGPRTQALNASTLLSRDGSELASCSNQPGMRVGGTRTAEGST
                     PVYKLDAGNMCWLWERAPLGGAKRVTLTVGRVAWRFGDEAAQAVVRPKASAAGELEIH
                     ADSCKGPLLARLPLAAAAQATGQSDLSTRVAAAPGNDARNLCIFATGDPRDGQWALAR
                     VAFSN"
     gene            34361..37615
                     /locus_tag="VFB37_09370"
     CDS             34361..37615
                     /locus_tag="VFB37_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO22700.1"
                     /translation="MSKVHRKHPVSIAVSMALLSAMTTSAMASPRAGAPVDSAGAKAP
                     AAQDVPATPADKEDTSKAATGTGKESEQSVELSTVTIVGVRASQMRAIELKRLAPDIQ
                     DSISAESIGQLPDTTITDALQRITGVQIDRNAGVGTQVDVRGLPEVGSMLNGEVFISP
                     DQITSQQPNFETLPATLFNQVDVIKSPTASQTAGGISGSLNLHTFRPWDLPHGFTFSY
                     SADGERGDVSRKNGPEASGLVAFNGDGRWGLLVSADFSDTTRSGPENEGANAGSSEGL
                     DQYGVILDGESAASAGAYNGFVGAWNGLPIPSQIVQNPDGSVDVNGDGKSNGLFMGSQ
                     NIGLYDSTIQRKRASGNASFQMDLGDGFTLTSDYFYTHQRQYNRNVGLQFNSTDWQGA
                     TYVPLQSMNTGATALSQYNTPPPDPAFSGSQIYTTQVYEKWPGDVESFSQIENTGSTA
                     QNANLQVDFNDGGNFRGSVRGIRETAKQAFVETDLNISDSDGCLWPNQLMDGVDPNSV
                     PCGTFIFPDQLGGNRVFNPIGIPEDSVPITANFNGRYISIGMPASMAANFANPNGWAM
                     KTIESSGDFDANAAITALRFDGHYDLHHGLHLDFGVRNSIHTATNSGYTLVTPVYAGM
                     GASDPNGCLVRYVGADVILAGDGNGGGCTAGNAQGYFRGGPISALQMTNTPPPLAKNW
                     QEYSNLLGSGINFWAVNPAAMDNPLAYWKSLYPQTETQEDPGNTWAVWMKESSAYLQT
                     DFGEELGAMPLSGNLGVRVIHTNLQVTQHLSGAPGLYGTESADIGTQVTRRGYYDVLP
                     SINLALDLTPQLTLRLAASKNMQPLDLVTWGGGLELNYSLVYLPNGNQLYQVAAGSSQ
                     GNPNLRPWRSTNYGASLEYYINPVSMVGLEVFRINVQSFIENGNVQNCTLPDEDGIVR
                     NHCITITEPVQGSGNSIQGAEIDYRQGFTFLPGLLSYTGMEFNFTYAPSKTGQTDLAG
                     HAIPFQDNSTESGNLILWFQSKRFEARAAYNYRSKRAVENQVGSIAGLEEYEAPQRYL
                     DASLSYKFSKYATVFVEGTNLTNEYQRFYLVWPSQEGHANFSERMYTVGLRGQW"
     gene            37609..40380
                     /locus_tag="VFB37_09375"
     CDS             37609..40380
                     /locus_tag="VFB37_09375"
                     /inference="COORDINATES: protein motif:HMM:NF037222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22701.1"
                     /translation="MVISRDVIGAGCARHARSACRIRAFFGVLVALAAPPLACAASAF
                     APTRVDGGAGSTTPIAQWEIQSSAKAQQGGTEVSSAGFSTSGWYPVSGRATVMTGLLE
                     NGIYQNVFYGDNLRAIEDPDASGSPFVIPWWYRTEFTLVAGSRALRTLLRINGIIAST
                     DVWVNGHPVADHTMIAGAYPVHELDVTRWVHAGTNILALRVHPGNPRTSLSIGWVDWN
                     PTPPDNNMGPWRGVDIVQTGPVELLFPQVTSALSLPDLARAALTVRVQAKNLDGVAHE
                     ATITGRVAGVSLQRTIHLAPGEAQTVAFSPKTDAGLGLSHPQVWWPIGMGAHPLYLLQ
                     MAAAVDGAASDRVSATFGIRSVSSYLTGKGYRGFAINGKPVLIRGGGWAPDMFLRDDP
                     KRLEAEFSYVRNLGLNAIRSEGKLEDAYFYDLADRNGIMILPGWECCDKWESAAKTGG
                     APWDAADEAVAAASTASEARLLRNHPSVIGFLIGSDNAPPAAIARMYVDILHAEDWPM
                     PIISAASDQATAAAGPSGMKMTGPYAWVPPAYWYAHRLGGAFGFNSETSAGASIPPLD
                     DLTRMLSPQELEALWKYPQVRQYHASAAWSPFATIAPFDTALARRYGAPRSLEDYVAK
                     AQLENYDNVRAQFEAFDAHMDAANRSTGVIYWMLNNAWPSLHWHLYDYFLNPAGAYFG
                     AKKANEPVHIQYSYDTRAIMLVNHTLNAEHGLQALIRVRNLDGSESYTRRLQDIDLAG
                     NSARQLTALPALSGLSRTYFIELELASANHQPISRNVYWLSTQPDDLDWAHSNWYLTP
                     VSEYADLTALQVLPAAKSEARATLRHEEADTIVTITLTVPASSSTVALFQHVSIRQSA
                     GGNLALPILWSDNDVTLWAGESLTLTAHFVPQGPGTPIVEVSGWNVPTRTVPVALESS
                     VGASPERSH"
     gene            40467..41312
                     /locus_tag="VFB37_09380"
     CDS             40467..41312
                     /locus_tag="VFB37_09380"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO22702.1"
                     /translation="MPAAPAVAKLARKFHFISGLPRAGSTLLAAILNQNPRFRAGMTS
                     PLADIMGVVMAEASSKNDFSFDVSDDQRVALLRGLVENFYAAETEASIVFDTSRLWCS
                     RMQLLGTLFPEAKVIACVRQLSWVLDSMERLVRRHPVGVSKVFRFDTNTTMYSRVEAL
                     TDPRGMVGFSFQATKDAFYGQYAPGHLLVLTYESLVRNPSAAMRAVYQFIGEPWFEHD
                     FDHVAYNADEFDARVGMPGLHTVRPKVEPIERTSILPREIFGRFVNESFWMDPKNNVS
                     QVPIV"
     gene            complement(41344..41862)
                     /locus_tag="VFB37_09385"
     CDS             complement(41344..41862)
                     /locus_tag="VFB37_09385"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HZO22703.1"
                     /translation="MRAQPLRWIVLTALAATAVLLWGCASNRSSPASQGAAVAANLGS
                     TGRSAAPLPGQPGCFRLSDFDGSWTVLNDSELIVYAPLYSQPYLIKLFAPVPTLRFRE
                     RLGFADTGHTGMICNDTMDTLVVPRWEPERVPIVAVRKLTESEARHLLAENHIRPPRG
                     PQPVRGNQPPGQ"
     gene            complement(41965..43965)
                     /locus_tag="VFB37_09390"
     CDS             complement(41965..43965)
                     /locus_tag="VFB37_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZO22704.1"
                     /translation="MSPASRIRISLLRLILGRRLANEEYVERKIGALEGVPAMGLDGL
                     GSSSYGPEAALTVLMPLGGAGLTYIGWVMVPILVLLVILYASYRQTIRAYPNNGGAYT
                     VSKLNLGTGASLIAASALMIDYVLNVAVGISAGVGALTSAMPRLHPHTLALCLATLVL
                     VSIMNLRGTADAGRACAIPTYLFLASFLGILGLGAYRTLVSGGHPLPLVAPPQPSHAT
                     AALSAWVLLRSFAAGCTAMTGVEAVSNGVSAFREPVMKRAHRTLGAICASLGLLLAGI
                     TYVVSGYGIAAMDETQSGYQSVLSQVASAVVGHGALYYIALSSVLCVLCLSANTSFVD
                     FPRVCRLVAEDDFLPRPFAIVGRRLVFSVGILYLTVTAGVLLVVFGGITDRLIPLFAI
                     GAFLTFTMSQAGMVRHWHRELRRATAAAVRQRYRAHLATNGIGAAATIVALLIIVVAK
                     FSEGAWITLLAIPATILVLRTVKKYYVRLDAQLRDERQLNVRSREPPLVLVTTPGWNK
                     LTDRALQFALRISPDILAVHFTDLRGSVADDEERGLRQCWARDVEEPARNAGLRPPQL
                     VRVKAPFRKMTGPLLQLIGEIQREQGNRLIAVLLPEVVKKHWWQYLLHGHRLRRLQAA
                     LLRYGGSRIVVMVIPWHLEEPRIEEGLEEEETPPTAASAADR"
     gene            complement(44065..44217)
                     /locus_tag="VFB37_09395"
     CDS             complement(44065..44217)
                     /locus_tag="VFB37_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22705.1"
                     /translation="MKILDTPVHFWDSGLLRQPWIARGSSFDGTALQDYCGAARGSPV
                     DSLVFV"
     gene            44291..44818
                     /locus_tag="VFB37_09400"
     CDS             44291..44818
                     /locus_tag="VFB37_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22706.1"
                     /translation="MARRLLAGMGLREIPGRMIQLTFHDAAGAPGPALESSDFRITGG
                     VLWSQVEHGLIACFAGGAWKHRGEYYPGISMAGACCLAFGIARDALVSEPISLFSVTG
                     TTLRANGVAIAEYDERQDLWQGLIRPISWQAMRLVSAAAASALADSSRAVTLNPWDYP
                     GSAWPAARSSSKYTT"
     gene            complement(44764..46773)
                     /locus_tag="VFB37_09405"
     CDS             complement(44764..46773)
                     /locus_tag="VFB37_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZO22707.1"
                     /translation="MSPPTTRRRVLLSAAAGLAAWAGKRAVAIVPQGKQRGVRLDAAV
                     QLSCREFDFADVTLLPGPFRDAMSRDLDYLASFDCDRLLSPFQQVAGLPRRAPSLGGW
                     ESQGLASHYCGHYLSAAAIMHAHTHDPRLARKVDYLLPRLAACQEANGREDPDFRGYC
                     AGIPESKPAFRQVLAGQMDFGQSHGLYSFHINGLWSPWYTIHKVMAGLRDVWLHMGRA
                     QARDILLAMTEWAAQFPRRLDLAQMQAMLVSEQGGMNEVLADVYSMTGDEAHRTLAEQ
                     FNQAAQLAPLMRRQDILDGVHSNQYIPRVIGIARQYELTGSDDYRTGAEFFWQNVVRQ
                     RTFVFGGHGNRENFFPIGDAWQQLSVGSAETCCTYNMLKLTSHYFSWNASMEAADFFE
                     RALINHILGSQDPATGMMTYYYSMQPGHFKTFSSKWDSCWCCDGTGLENHARYSRGVY
                     YHERDTLWVNLYLASQLQWREKGLTIRQHTRFPESNSVRMQIACARPVRATLRLRHPH
                     WSTPAMLAKLNGRPIDTGKPGTYLDITRHWRDGDVLEIELRTPPRIERMAHHPSKLAI
                     LSGPIVLAGVLGDALMAPPIPYATVDQYQWEKVPDPVRAPTLAIGGRPVEEWVKPDAA
                     EPLVWHTQGAGRPADIRLVPLYRVARERYVVYFDEERAAGQALPG"
     gene            47034..47804
                     /locus_tag="VFB37_09410"
     CDS             47034..47804
                     /locus_tag="VFB37_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO22708.1"
                     /translation="MTGANSGIGRATAIAMGQAGADVVVNYVTGEDRAEAVVEEIRRG
                     GVRAYAHCADVACEDQVKEMFTRMVADLGTIDILVNNAGRQKDAQFVEMSLADWNAVI
                     SVNLTGQFLCAREAIREFLRRGVVPSVSCAAGKIICMSSVHEVIPWAGHANYAASKGG
                     VMMMMKSLAQEVAPHHIRVNSIAPGAVRTPINTAAWSTPEAYRSLMTLVPYKRIGEPA
                     DIARTAVWLASDESDYLVGSTVFVDGGMTLYPGFTENG"
     gene            complement(47813..48634)
                     /gene="dsbG"
                     /locus_tag="VFB37_09415"
     CDS             complement(47813..48634)
                     /gene="dsbG"
                     /locus_tag="VFB37_09415"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HZO22709.1"
                     /translation="MKPRSLRRCLVGFCVSLLALPALAATHASSPPLPPMLASLVAAG
                     KLQVVSQFGTEVPGLTGYIVHNQEGTQVVYGEHGYLFVGHLISPHGNDLTDNYRERYS
                     PKPDYGSVVGKLESGGHLINEGRAAAPMLYVFADPNCIFCYHFYKMAEPLVASGRLHI
                     RWVMVGFLHADSLARATTILSASNPLGALHADEDRFDTAHESGGVAPARTQDKALQGV
                     LQAHLSAMDAVGGVGTPTLLYRDERGHWGAVVGLPSSDWLTAYVAGKHLPANPNG"
     gene            complement(48740..49345)
                     /locus_tag="VFB37_09420"
     CDS             complement(48740..49345)
                     /locus_tag="VFB37_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22710.1"
                     /translation="MAINVTDLNNRCVTTSTVGYMALALTSWMLSMTAAGWYPQIYSA
                     GSALMLPFALLLTIIGILAYLHGRSLDSIVFFAFAVLFTAGHSGMAAIAAGAPSPMSY
                     VGWFWIVWTVFFAYTWLGSFRAGLPRQLFLLGITLTFLAKAIYGWTDLRALQLISGYL
                     GLISAIIAAIISASDVILFGREAHSPNDDHVDHAAGHPHTA"
     gene            49824..51332
                     /locus_tag="VFB37_09425"
     CDS             49824..51332
                     /locus_tag="VFB37_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22711.1"
                     /translation="MTHTDVVTYRDDAGRTGENLTESLLTPTNVNSSSFGLYRVLAVD
                     GKVDAQPLYLAQLPIAGGTHNVVLVATEHDSVYAMDPVSGDTLWHVSLLPSGETPSDD
                     RGCNQVSPEIGVTSTPVIDRSAGPHGAIFVVAMSKDSLSNYHQRLHALDVTTGAELFN
                     GPTSINPSYSSPAGGTKTFSPGQYAERAALLLENHTIYTSWTSHCDVQPYSGWIVGFS
                     ESTLAQTAVLNVAADSDAGPSIWMSGDGLAADSSGNVYLATANGMFDTTLTSGGLPQN
                     GDYGNSFLKLSTAGGGLAVADYFSPSNEVAESNADQDLGSGGVMLVPDVTDSGGVVRH
                     LLLGAGKDGNLYVINRDNMGKFNPSRDMTWQQLNGIFPSTVFSTPAYFNGTVYYGDVS
                     NTLKAFPVTAARLASSPSSETTTIFGYPGTSPAVSANGASNGIVWAVESARTAVLHAY
                     DAANLTHELYNSSQAANARDQFGAGNKFMTPTIADGMVFVGTPNSVAVFALK"
     gene            complement(51342..52904)
                     /gene="hutH"
                     /locus_tag="VFB37_09430"
     CDS             complement(51342..52904)
                     /gene="hutH"
                     /locus_tag="VFB37_09430"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZO22712.1"
                     /translation="MSAARAAARRQTVRTESFSLTSLRTAYSLPVAVTLSRAQMARVR
                     RSAATVDRMIARGGAVYGVNTGFGLLAQTRIPTGQLAELQRNLVLSHSAGVGPWLPDA
                     VVRLVLVLKIGSLAQGFSGVRPPTLLALARLLETDTYPCIPSKGSVGASGDLAPLAHL
                     AAALLGVGQVRTGGRVMTAAEGLRRIGLQPLELAPKEGLALLNGTQVSTALALAGLYA
                     IQDVFGAALVSGALSVDALKGSDVPFDDRIHVLRRQPGQRRVAKALKRLLGGSRIRAS
                     HLTCERVQDPYSLRCQPQVMGACLDVIESAGQTLLREANAVTDNPLVFAADGEVLSGG
                     NFHAEPVALAADQLALAAAEIGSLSERRTALLIDPKMSGLPAFLIKESGVNSGFMVPQ
                     VTAAALVAENKTLATPASVDSIPTSAGQEDHVSMATHGARRLLEMATNAACIIGIELL
                     AAAQGIEFHRPLTSSRPLEAALRRLRRAVPRYDRDRFFAPDIEAARELVSSGALRELV
                     ETVEPGIATASG"
     gene            complement(52901..54592)
                     /gene="hutU"
                     /locus_tag="VFB37_09435"
     CDS             complement(52901..54592)
                     /gene="hutU"
                     /locus_tag="VFB37_09435"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZO22713.1"
                     /translation="MTELSPSARRTIRAPRGSRLTARSWLTEAPLRMLMNNLDPEVAE
                     RPEDLVVYGGIGKAARNWECFDAIVAALTRLGPEETLLVQSGKPVGIFTTHADAPRVL
                     LANSNLVPRWASWEKFNELDRKGLMMFGQMTAGSWIYIASQGIVQGTYETFVELGRRH
                     YQGDLRGRWILTAGLGGMGGAQPLAATMAGASLLAIECRQSRIDKRLATRYLDRQARS
                     LDEALAMIEESTRAKKPISVAVCGNAAQLVPEILRRGVRPDAVTDQTSAHDPVHGYLP
                     MEWTVEQWEALQKSDPQRVAAEAGPSMATHMRALVEFRRRGIPTFDYGNNLRQMASEN
                     GVTDAFEVPGFVPEYIRPLFCRGIGPFRWAALSGDPQDIARTDAKVKELIPDDPHLHQ
                     WLDMAGSRIAFQGLPARICWVGLGQRDRLGVAFNQMVASGELSAPIVIGRDHLDGGSV
                     ASPNRETESMRDGSDAVSDWPMLNALLNTASGATWVSLHHGGGVGMGFAQHAGVVIVC
                     DGTKEAERRIRRVLWNDPATGVMRHADAGYPEALACAAEHGLQLPMSGTRRGAAQ"
     gene            complement(54771..55262)
                     /locus_tag="VFB37_09440"
     CDS             complement(54771..55262)
                     /locus_tag="VFB37_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22714.1"
                     /translation="MNTGRRERQEAGGFAARLRQVLKTHGSASGLAKAIARSEGAVRK
                     WLRGESEPNVTDLRTLCQVTQTRIEWLVSGEGPREAEAQSNPDTLVRESAPPYRHPSH
                     GTLDLPLLEAVMAAVDEQLRAAGLDLSPAKRSALIAAAYDLARERNAVGAEAVARLVR
                     LTH"
     gene            55521..55706
                     /locus_tag="VFB37_09445"
     CDS             55521..55706
                     /locus_tag="VFB37_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22715.1"
                     /translation="MRLRQVQSAVAVAVAALRRQNCELDEDIATLLQRCVCDRIEDQL
                     HELDAARHLLAARLRRV"
     gene            55743..55824
                     /locus_tag="VFB37_09450"
     tRNA            55743..55824
                     /locus_tag="VFB37_09450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55777..55779,aa:Leu,seq:tag)
     gene            55856..57199
                     /gene="tig"
                     /locus_tag="VFB37_09455"
     CDS             55856..57199
                     /gene="tig"
                     /locus_tag="VFB37_09455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZO22716.1"
                     /translation="MQVSVSATSGLERRMEVAVPAASVTTAVEQRLKEISRTARLKGF
                     RPGKAPLPVVRKQFGEQVRAEVLGDLMRSSLAEALSEQKLTPAAGPRIEPIAVDPGND
                     LKFAATFEVLPEIQVKSPAAIAVERPTATVEESDIDAMLESMRRQRPVFTAVDRPARD
                     TDRVTVDYTGRIGGEPFEGGEGKDVPIIIGSRQSRPELEEGLKGAVAGESRTVAVTFP
                     AGTSNPTLVGKTAELALTVKTVEEQSLPAIDEEFCRNFGVEEGGIAALRAEVRKSMER
                     ELADVIRNRVRTQVLDALYAENPIDIPRALVEEQVQQLQIDAARRMGVREASQLPPRQ
                     PFEAPARKRAALGLLMGQILKTESITVDRQRLQARVEELAASYPNPEEARRAYQHNAE
                     ALRQIESVVLEDQVIDWLLERAKVNEVPMTFRDITGFGQKPDNSAESQSGEKDPS"
     gene            57196..57840
                     /gene="clpP"
                     /locus_tag="VFB37_09460"
     CDS             57196..57840
                     /gene="clpP"
                     /locus_tag="VFB37_09460"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZO22717.1"
                     /translation="MNERALNLVPIVVEQTARGERAYDIYSRLLKERLIFVVGPIDDY
                     MANIVVAQMLFLESENPEKDISIYINSPGGVVSSGLAIYDTMQFVKPDVSTICIGQAA
                     SMGAVLLAAGAAGKRYCLPHARVMIHQPLGGFQGQAADMEIHAKEMLETRDRLNKILA
                     KHTGQSVDRIKQDTDRDRFMSWEEAVRYGLIDRVLEKRGEIPPDAAGPKAGKPA"
     gene            58035..59344
                     /gene="clpX"
                     /locus_tag="VFB37_09465"
                     /pseudo
     CDS             58035..59344
                     /gene="clpX"
                     /locus_tag="VFB37_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
     gene            59540..61948
                     /gene="lon"
                     /locus_tag="VFB37_09470"
     CDS             59540..61948
                     /gene="lon"
                     /locus_tag="VFB37_09470"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZO22718.1"
                     /translation="MLPLRDVVVYPHMVIPLFVGREKSIQALDVAMKGDKRIMLVAQK
                     QADVDDPKGEDLYRIGTVATILQLLKLPDGTVKVLVEGVDRARIDKLTAGDYYAAELA
                     LLPDVEAYEEREMDVLARSVISQFEQYVKLNKKVPPEVLTALAGIEHAGRLADTVAAH
                     MSLKLAEKQKVLEILDIRKRLEHILVAIEGEMDVLQIEKRIRGRVKAQMEKSQREYYL
                     NEQMKAIQKELGEMDEGGNEVTELEQKIARAGMPKEAREKATSELNKLKMMSPMSAEA
                     TVVRNYIDWLVKVPWKKRTKVLKDITRAEQVLDEDHYGLEKVKERIVEYLAVQQRVEK
                     LKGPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGGVRDEAEIRGHRRTYIGSLPG
                     KIVQNMSKAGVHNPLFLLDEVDKMSMDFRGDPSSALLEVLDPEQNTTFNDHYLEVDLD
                     LSEVMFVCTANSMNIPAPLLDRMEVIRLPGYTEDEKMNIGRRYLVPKQMKSNGLKESE
                     LKMSDAVLLDIVRYYTREAGVRNLEREIAKICRKAVKQMLTHKKDETSVSVTPRNLDK
                     FLGVRRFRYGKAEDMNRVGQVTGLAWTEVGGELLTIEAAVVSGKGKLQHTGQLGEVMQ
                     ESIQAAMTVVRSRAQVLGLDGEFYQKHDVHLHIPEGATPKDGPSAGIGMCTALVSALT
                     KIPVRSDVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIKTVLIPDENEKDLVEIPDNI
                     KGNLEIKPVKWIDEVLALALTQQPVPAAQTEAPAAPPPGPTAEEEKRGRRNGRRLKQV
                     VPAH"
     gene            62083..62158
                     /locus_tag="VFB37_09475"
     tRNA            62083..62158
                     /locus_tag="VFB37_09475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62116..62118,aa:Val,seq:tac)
     gene            complement(62272..62637)
                     /locus_tag="VFB37_09480"
     CDS             complement(62272..62637)
                     /locus_tag="VFB37_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22719.1"
                     /translation="MPIGYARARDGKLALHVPRWICAALVIAGFWLILADLPHFALCQ
                     LAGSIAIVGAVVSWEHQYGLPRFRVPLLLGDASYSIYLVHLFAFGLVRAAWGRMHGGN
                     ALAFAALAAGSAIILALIT"
     gene            complement(62952..64412)
                     /locus_tag="VFB37_09485"
     CDS             complement(62952..64412)
                     /locus_tag="VFB37_09485"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZO22720.1"
                     /translation="MSARTLMVQGTTSDAGKSTLVAGLCRVLRRRGVRVAPFKPQNMA
                     LNSAVGIDGGELGRAQALQAQAAGIPPTVDLNPVLLKPSTDQRCQVIIHGKALGEMGA
                     WDYHEFKRSAREAVLASYHKLRAQYEVIVVEGAGSPAEINLREGDIANMGFAEAVDCP
                     VLLIADIDRGGVFAHLVGTLALLGESERQRIRGFVINRFRGDLSLLQPGLDWLEHHTG
                     RPVLGVLPYLHGLQLDAEDGISREAAAAPARTAEMLRVVVPVLPRISNHTDFEPLARH
                     PQVELRYIGPEAAPPPADLVILPGSKSVRADLEWLRANGWPAYLERHLRYGGKVLGIC
                     GGLQMLGRRIADPEGVEGAAGESPGLGWLDLTTVLLPGKRLCNVSGHLLLEGARVKGY
                     EIHAGRTEGAALHRPAVRLDDGTVDGTVSADGQIMATYLHGLFEETAACTALLRWAGL
                     TRPLTIDHGARREQMLDRLADTIEGECNLERLLALL"
     gene            complement(64419..65123)
                     /locus_tag="VFB37_09490"
     CDS             complement(64419..65123)
                     /locus_tag="VFB37_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZO22721.1"
                     /translation="MPDLLAFPVTGRWPPAFPDRLQLYSVPTPNGVKVSIMLEETGLP
                     YEPHRVSFDAHEQLSPEFLSLNPNNKIPAIIDPDGPGGQPLPLFESGAILVYLAEKTG
                     QLLPRDPRGRYLTLQWLMFQMGGVGPMFGQVGFFNKFAGRSYEDKRPRDRYVAEAKRL
                     LRVLDGQLKSRSWLAGEEYTIADIATFPWVRNLIGYYEAGELVGFGEFANVSRALDRF
                     LARPAVARGLEIPRSG"
     gene            65482..66810
                     /locus_tag="VFB37_09495"
     CDS             65482..66810
                     /locus_tag="VFB37_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22722.1"
                     /translation="MAQSLDSRPGGSVPAAADLELERASCDLQHRMAGLESANARLQQ
                     ALDVSEALRAEAESANRLKDEFIATVSHELRTPLNTIRLWSHMLAEGGLCAADAAQGA
                     QVLERSALAQQHLIDDLLDISRMSRGQLRLELRSTDLIQAVAAAAAQIKPLAQARQIR
                     LATDLAGGTAMVRADADRIQQVAWNLLTNAVKFTPAGGRIDVWLHRVDDRMELEVRDT
                     GMGIDAHLLPHVFDRFRQGVASARRQGGLGLGLSIAKQLAELHGGSISAESAGPGRGA
                     LFRVCLPLSRAASGIQESVGGRCRTAPPNLSKIEVLIVDDEPFAREALARLLEQNGAS
                     VRAVGSCAAAREALTLRRPDVLVADIRMPEEDGYSLLRALRREEQEHHEQRIPALAVT
                     AFARLEDRQRALAASFDEHLPKPVDPDRLLAAVAMLARRPAKNPCGGDTR"
     gene            complement(66779..67633)
                     /locus_tag="VFB37_09500"
     CDS             complement(66779..67633)
                     /locus_tag="VFB37_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZO22723.1"
                     /translation="MVHVIILAIVQGLAELLPVSSSAHVIVAEKLMGLDPSAPEMTLL
                     LVMLHTGTMLAVIVYFWPRWRRAFFSSVPEFRNFAAAVIVATLLTGIVGGGIIEFIEK
                     VLMRHEAHAWVEQLFSRLDLIAVALAAAGAFILIAGLYENRHRDIAAVPKLTPAQYSA
                     IGAIQGLCVPFRGFSRSGATISTGLLARVRRSSAEDFSFALAVVLTPPADAREIWRLY
                     QAQHHHLAGVLPAATASILGVLFAFLAGLLALKWLSRWLETGRWHFFGIYCLVAALVI
                     AYLHHTGF"
     gene            67804..71055
                     /locus_tag="VFB37_09505"
     CDS             67804..71055
                     /locus_tag="VFB37_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22724.1"
                     /translation="MTAADRQSKAPRIASLSALAIACAAVAAAGLAGPAAAAQGVATA
                     AEVPFPQGHAAPGLHGAPASAYSQLRWRLVGPFRGGWATMAASVPGSPDTFYFSGAGG
                     GIWKTIDAGRTWQSVGDSLPPAVGALAVAPSAPATIYVGTGQVSLRYDVAAGRGVFKS
                     TDGGKTWKSLGLAATRSIGRIWVDPHNPDTVVVAALGHLFGPNPQRGIYRSTDGGKTW
                     VHSLAINDETGVVDLGGDPTKPNLLYAAAWQTRDYPWLDYFEPHEGPGSGIYRSTDGG
                     ATWTRLSGNGWPQGPLGRIGIAVTHTAQGARIYATVASKTAGGVWRSDDGGDHWVRVN
                     SDAETFGNPYFSRLEADPHNPDVIYSAGQSIRRSSDGGKTWTIIKGAPGGDDYHFFRI
                     DPEHPDHWITASDQGAVISVDNGKTWSSWYNEPTGQFYHLGADNRFPYWIYSGQQDSG
                     SIGTASRSDYGAITFRDWHPVGGDERDYMLPDPDDPNLVFGSGMGGRLSRYDTSTGQV
                     SNVTPWPVNSYGADPTKVKYRYGWVTPMAFTAAKPTALLFGAQVLFRSTDEGNHWDII
                     SPDLTGKEDDAKDCNGQVAGEQARACGYGIISAIAPSSKSPDLIWVGTDSGLIQLTRD
                     GGQHWQNVTPRGLQPWQKVSAIDASPLDPATAYASVDDHRSDHFRPHVFRTHDYGKSW
                     TEVDAGLPAGEFVPVVRADTVRSGLLYAGTAEGAYVSLDDGDHWQSLRLNLPKATVND
                     LLVHGNDLIAATQGRAIWVLDDLTPLRQLSAAVLGAPAHLFAPQIAWRVHPNNNKDTP
                     LPPETPAGRNPPAGAVIDYWLGKDAHGPVTLEIYSFTGQLVRRFSSDEAPAHIQADRY
                     FDEEWLQPPQRLSDSPGAHRFVWNLRYPRPQATSYDYSIAAIYGEDTPTSVQGPFVLP
                     GIYSIVLTAGGEHYRAPLVVQLDPRVHTSNADLHALLAFSQSLCAALERTSTAYEQEK
                     SAHDQLESLAQRLTAAHGDRALLRDVEKLRDETAARRDDTDLGTISSRISALESDAES
                     ADRAPTAADTEMLGNESHALDEALRAWHEDQAAIGKLNARLRRARLPAVSTAG"
     gene            complement(71058..72380)
                     /locus_tag="VFB37_09510"
     CDS             complement(71058..72380)
                     /locus_tag="VFB37_09510"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HZO22725.1"
                     /translation="MRKLILLGLTQALLLLSGCGGSGTVVGSAASTGKGSGTGSGGGQ
                     TSNVVVMTVDAGPDVTATSGYDVDTPFITVTVCAPASTTNCQTIDHVEVDTGSYGLRI
                     ISSVLTPSLASALHQELATGGNPLVECTQFIDGFSWGPVKTADVQIAGELASNVPMQV
                     IGDPSFQNEIPTACSGTGPEEDTVAEFHANGILGIGPFGDDCGGGCASTAANTFYYTC
                     PANGTGTGSCGGVAVSEPEQVTNPVVSFQTDNNGVIVQLPAIPDPGGAPTATGSLTFG
                     IGTEGNNGLGSATVLTSNPSTGDITTTYNGTPLAYSYFDTGSNAFYFDDNSIPTCPSS
                     GSGTSSSTWFCPTTELSLSATNTGRNGMVSTVHFQIANANMLFSTYQGNTALADLGAS
                     AGDESGYCPNSATDCSFDFGLPFFFGRNVYVAIAGANTEGGPGPYFAY"
     gene            complement(72403..72891)
                     /locus_tag="VFB37_09515"
     CDS             complement(72403..72891)
                     /locus_tag="VFB37_09515"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HZO22726.1"
                     /translation="MKVFSSTLSAALASGALLLACGSMVPAKAALGDKIASVQTDQAS
                     MKGQLRTRSGTGYSVQEITTASGTLVREYVSPAGVVFAVSWSGPAMPNLQQTLGTYFV
                     QYTASVRSQLTARGMHPGHNHVEVHSPSLIVHAGGHMRQYFGLAYVPSLIPQNLSISD
                     LH"
     gene            73155..73231
                     /locus_tag="VFB37_09520"
     tRNA            73155..73231
                     /locus_tag="VFB37_09520"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:73189..73191,aa:Asp,seq:gtc)
     gene            74065..75036
                     /locus_tag="VFB37_09525"
     CDS             74065..75036
                     /locus_tag="VFB37_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZO22727.1"
                     /translation="MERRLELHVFPYIGERDVRLLTARELLEVLRRIESRETYDLAHR
                     VRSICSRVLRYARATGRQCEDVASDLVGSLTPVESEHMAAIVEPARIGALLRSIEAYS
                     GEPLTRLALKLMPYIFPRPIEIRTMEWAHLQLHGTSPEWRVPWRRMKMRAPHIVPLSR
                     QATDILREVRLLTGARRWVFPQLRNPERPMSGCCITAALRTMGYAGTEMSWHGFRALA
                     STQLHELGWNDSWIEAQLSHADRNKVRGSYNHAQYLPQRRTMMQAWADQLDSLRGRGE
                     ADVSQVRWRPSETATDGFQQVESERALSFQAEAIEALRAIIALCPKR"
     gene            complement(75669..75824)
                     /locus_tag="VFB37_09530"
     CDS             complement(75669..75824)
                     /locus_tag="VFB37_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HZO22728.1"
                     /translation="MNLHLTIGPLVSLVAGILILLVPRLLNYIVAIYLIIIGLLGLFG
                     SGTLHLH"
     gene            complement(76205..77377)
                     /locus_tag="VFB37_09535"
     CDS             complement(76205..77377)
                     /locus_tag="VFB37_09535"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZO22729.1"
                     /translation="MSHIVQRVLELDPQTVRQQLADVVESFQGRHRRLLATFATRTDT
                     LELAHSTHTSLTDAQRQLIGAYFLHEYSFEAAALFNPSIVAHPDQSGVPGGERRFILS
                     LRAVGEGHVSSLTFRSGVLAADGTVRLEPIAQLASIPHLQRRVPGALEDCFEVAFEPD
                     SSISERVIFPVTEAQSNGIEDARFVEFDDGGRKTFYATYTAYNGRAIRSELMETTDFE
                     RFKMTPLTGSAARNKGMALFPRRLNGQYAMIGRQDNENLYLIYSDDLYSWHEGIAILR
                     PQFPWEFVQIGNCGSPVELDEGWLLLTHGVGPMRRYSIGAVLLDKQDPSKVLARSPEP
                     LVRPELSEREGYVPNVVYTCGAMRHGDLIILPYAVCDTFSNFASIEISALLDAMRS"
     gene            complement(77540..79825)
                     /locus_tag="VFB37_09540"
     CDS             complement(77540..79825)
                     /locus_tag="VFB37_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO22730.1"
                     /translation="MSPLGRLAFVGNSLPRRCGIATFTTDLQQAIEQLRPHESSCIVA
                     MTDPDHTYDYPPAVRLQINDRNLEDYEQAAHALNMARCEVVSLQHEFGIFGGEAGGHV
                     LALTSRLTMPLVTTLHTVLSQPTSAQRRVLGEIVDTSSRVIVMAEKGRELLRTVYRVA
                     VGKIEVIAHGTPDCAFLEPDEAKAALGFGGRPIILTFGLLSPSKGIEVMIDAMPEILR
                     TSAAAVYVVLGATHPNLIRDQGEAYRDGLVRRVRELGIEDHVVFLDQFVDQATLLRFI
                     SMCDVYVTPYLNEAQMTSGTLAYSFGLGKAIVSTPYWHARELLGEGRGILVPFGDAAA
                     LGRETAALLSDERRRLAMRERAYAVGRSMIWPRIAEHYRAVFASARRSDLRQITARQD
                     AGGPPRDQEASRAMPIGHFLSLCDDTGLLQHATHCVPDRSHGYCTDDNARALLLACAL
                     GKPGETPLPDTVTSRFAAFLQHAWNRDTRRFRNFMDFDRRWHGESGSEDCHGRALWAL
                     GECAQSDREQSRRQWAASLFAEALPAVERFCSPRAWSFTLLGLDAYCAAVAQDATARR
                     LRGVLADKLIASLTAVETPDWAWFEEGLAYDNARLPQALIVTGSATRASNYLSAGLRA
                     LHWLMSLQTTPAGLFRPVGSKSFGAKRTRPLAFDQQPLEATASISACLAAWRVASDVT
                     WQAHAARAFAWFRGSNDLSLSLIDPETGSCRDGLHPDRANENRGGESVVSYLLSFAEM
                     RQLGHLSGEQTKRPLQLAASA"
     gene            complement(79822..80850)
                     /locus_tag="VFB37_09545"
     CDS             complement(79822..80850)
                     /locus_tag="VFB37_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HZO22731.1"
                     /translation="MGAALNAIRHHVPSANVVNLETFMAVEQARVRLVPKPWGRTDLR
                     PWNVHHDAAATMGEVWFERSDAQAPCPALLLKLIFTSAPLSIQVHPDDEYARSIGLAH
                     GKCEAWYVLSATEGAQIALGLKHRLTAAQLRTSIGDGSIAEQVQWHTVRTGDATLVPP
                     GTIHAIGAGLVIVEIQQRSDTTFRLFDYGRKRELHVDAAVAAANADRAERQPPERKLT
                     AARTLLAASPHFVLERLTLPPESVWNLNATGEMWLFILAGRAWAGAIDVQTGSAVFLE
                     ADGTRVDVGSDGLELLVAYAGAVSAPDLLRGLSGEHALDMARDRQRAHFRAKAMSVLG
                     PHPAGETR"
     gene            complement(80960..81226)
                     /locus_tag="VFB37_09550"
     CDS             complement(80960..81226)
                     /locus_tag="VFB37_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZO22732.1"
                     /translation="MSLFSWIVLGLIAGFIASKIVNRTGEGVIVDILLGIVGAIVGGW
                     LFRLFGMPEVSGLNIYSTVVAIIGAAVVLLVYHTMFRGFRDRRV"
     gene            complement(81654..81983)
                     /locus_tag="VFB37_09555"
     CDS             complement(81654..81983)
                     /locus_tag="VFB37_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZO22733.1"
                     /translation="MKTYLATGCAIVATLLGGSIAATAQDSHSDAAHAETYVKDSAIT
                     TKIKTRLAAEHITSLGDIHVNTDKDGVVWLSGSVKTQRAADKAVSIARSTAHVKRVHS
                     EIKVVRD"
BASE COUNT        14526 a        26530 c        26951 g        14074 t
ORIGIN      
        1 tcgccgtgct cctctccgcc ggcaaccggg tgttgtcgcg tgcccagctc acggagttgc
       61 tgcggggccg ggaggcggac cccttcgacc gtagcatcga tgtacgggtc agccggttgc
      121 ggcagatcct gggcgatgat gcgcgttcgc cgcagatcat caagacggtg tacggcgagg
      181 gttacgtaat cggcgtgccg gtcgagagca cctgagcgca gccggcagcg atcatgcgca
      241 tcccgctctg gccgcaatcc ctgttcggcc ggctgatcgc agccctggtc atcgccgtcg
      301 tgttggcaca ggcggcgagc ctctatctct tcgcgcacga ccgcgagcgg ttcgtcctcg
      361 acagcagcgt gcgggagtgg tcgcaccgca tcaccgagat cacgctcctc ctcgagcaac
      421 ttccaaccgg gcagcgcgcc gagacgattg cccggctgaa ggagcgggga ggcagactcg
      481 ctccgcggca cggactgcgg atgagtggac cccgggatgc cacatggtcc gacggcccgc
      541 tcctgatgcc gccgtctcac tcgagtccgg cgttcggcgc tgcaccgcgt gccggcgaat
      601 ccaacgccga catacccgcc acgcaggagc gcgcctattc cggccaccac cccatgctgc
      661 cgccccacgc gcatcgattg atgcgcgtgc tgcaccgaca aagggaggag accttctggc
      721 cgcgcatctt catccagttg ccattcgcct cgaacgcgca ggaagtgctg gccgagcagc
      781 tgcgcgccca gctcggcaaa gactacgagg tgagtgtcca gccggccgcc gcaccggcgg
      841 cgagcgtcat tccgattcca tcgccattct tcggcatgcc ggcgcaacgc ccgaccgaat
      901 cgtacgatgt cgatgtccgt ttgcctgaca gctcgctgct cgtctaccgc gtgacccgcg
      961 tctcccccgg ggcgccgctg ccgccgagcc tcatggtcaa cctgatcctg ctcgtggcgg
     1021 tgctcgtcgt tgcgctctac gtgacggccc gcagcatcac ccgcccgctg tcgcggctgg
     1081 cgcttgcggc cgacaatctc gggcgcagcg tccgccagcc caagttggaa gagcaggggg
     1141 cgagggagct gcgccgtgcg gcgcgcgctt tcaataccat gcaggatcgg ctgcatcgtt
     1201 atctcgacag tcgcacccgg gtgcttgccg ccatgtcgca cgatctgaag acaccgctca
     1261 cccgcctgcg gctgcaggtc gagacccaga tcgaggaccc ggcgctgcag gcgcggttcg
     1321 gcaaggagct cgatgagatg gagggcatgg tgcgcggtgc gctcgcgctc tttcgcggct
     1381 tgaacgatga ggaggcgctc gagccggtcg acatcaacct gctgctcgag accgtgcgcg
     1441 gcgagttcac ggaaatgggc aaggatgtga cggtggaggg tcatgcgctc gggccgttgc
     1501 cgggcaagcc ccaggcgctg aagcgttgca tcacgaacct cgttggcaac gccgtcaact
     1561 tcggcggccg tgcgcgcatc ctcgtgcacg acgggacact ccttacgatc agtgtctgcg
     1621 acgagggtcc gggcatccct cccgaggagc tcgagaaggt gttcgagcct ttctatcgcc
     1681 tggagtcctc gcgcaaccgc gacaccggcg gcacgggcct gggactgagc atcgcgcgcg
     1741 atgtcgcgca ggcgcacggc ggctccctgg tgctcgaaaa tcgtcccgga ggggggctgc
     1801 aggcgcggct cagcctgccc cgcggccgtt gagcccggct gcagccggcg ttaaagatcg
     1861 cttacaagtg tgagtcggcg cggggggatg tcttaaacat tgcttactgc aggtgccggc
     1921 ggtgaggctt agagtgacca cctgagccca acttggagca tcacatgatt tcacgcagcg
     1981 ttgtcatgat ggcgatgttg gctgtcgccg gtacggcgct cgcccaggcg cccccttcgc
     2041 catcgccgca atggcagggg catcatcagt ggaaaagctc cgccgagtgg cggcagaaga
     2101 tggagcagcg ccgcatgcag cggctgactg tcctcctcga cctgacaccc gcgcagcagc
     2161 agcaggtccg cacgatcttc tccgacgagc acgccaagat gaaggcggcc atggaaccca
     2221 tgcgggaagc gatgaagcag atgcgggccg cccatgaggc cgcccacaag gatgcgctcc
     2281 agcggctggg atcggtgctg agccccgcgc agatgaagaa gctcgatgcc ctcatgccgc
     2341 cacggggcat gatgcgaggt cactggatga tgatgcacgg catgggccac ggggaaaaaa
     2401 tggggcctcc tccgggatcg gacgagggcg ccggatcgaa cccgccgtga ggattcagcg
     2461 ggggagtgtt cgcagctcgt agcgcccggc ctcgcactcc aggtagctac cctgctcata
     2521 ccaggcgccc agcacgatgc gctggacggg ttggccttcc atcagcgtat cgtgtatggc
     2581 cggcctatgc gtgtggccgt gaatgatgcg gcggacgcgc gcggctttga aggcggctgt
     2641 cacggctgcc gggttgacat ccatgatctt cgggatggtc cgtgaggtat gagcgcggct
     2701 gccggcgcgt accccatcgg caagcagctg tcggtcagac agcggcagcc tgagaaaccg
     2761 tctctgccac acaggcgctc tcactatgct gcgcagctcc tggtacgggt gatcatcgac
     2821 gcagagcgca tcgccgtgtg tcagcagcac cggctcgccg gctatctcgg caatcaccgg
     2881 gtccggcaac aactgacagc cggtctggag gctgaagtcc tgaccgatga gaaagtcccg
     2941 gttgccgtgc agcaggaagc aggcgattcc gccggtcgtc agtgcgcgta acgcggcgag
     3001 cacctcggct ctgtcgctgc tgagatcatc atcgccgacc cagctctcga agagatcgcc
     3061 gaggatgtag agcgcgtgcg ttcgcgcagt ggaaccgcct acgtgtgtct gcagaaactc
     3121 gaggaactgg cgttgtgcct ccggcgccgc ggggtccaga tgtacgtcag atacgaagag
     3181 ggaacgcgtc ggcatcgagc tcggagcccg cggtattgcg gggtccgaaa ccgccgtcat
     3241 atcggcggtg gctggtccgc ctcgcccggc ggtgtctgcg gcggcggctt cgtaccctgc
     3301 gacttgtcac ctgccggtgc cggcggcgtt cctggcggag gcgttgcagg ttgcgggctc
     3361 gtcgatgccg gcgcgcccgg ctggccaggc tgcggctgtg ggctcgaggc gggaggctgc
     3421 gaggaggggg gcgatcccgg ctgaggtgta cccggctgcg cggatcctga tggcggcgtc
     3481 tgcgtcggcg gggtgcctgc cggcggaggg cctgactgct gcgcgcctgg ctgcgggctc
     3541 ggcgcaggtg tcgtggtttg tgcggcgccg acgagctcga cactttgaat gatgaccggc
     3601 ttcagcggcg catcggactt gaagggaccg aaggtccccg tcgggacgac gccgatccgg
     3661 tcgatcacgt ccatgccctc caccacctgc ccaaagactg cgtagcccca gcgggtgggc
     3721 agcgggtcga gctccggatt gtcgaccagg ttgatgtaga actgggcgtt gcccgtgtgc
     3781 gggccggcgg cgcgcgcgag gccaacggat ccccgcttgt tctgcagtcc gttccctgac
     3841 tcgttgtcga ccggcgggta ggtgggcttg agcgcgtagg gatccgtcgc cgcgtggccg
     3901 ccgccctgga cgacgaagtt ggcgacgacc cggtggaaga gggtgttgga ataaaagccg
     3961 tcgcgcacgt agtgcaggaa gtcggccacg gtgagtggcg cccgatcggg ccgcagctcg
     4021 atgacgaagg agcccatgtt cgtggtgacc cggacctgag ggttcggggc ggggggagcg
     4081 tccgctgccg ccgccggccg tatgaggaga aggcacgcga gagcggccgc caggacgagg
     4141 agaccaccga gacccgctct cgtgatggcg agtcccccag aaaacggttt catcatcagg
     4201 gtccggtacc gggcgcgcat cgggtctcca tcgcagtagg tcgtcgaaac tggaatctta
     4261 gcagtgccgg gggctcggga gcttgccgtg tgtcgcgacc gcggcaaacc ccgtaccatg
     4321 tcatgcatga ccggaatgga cagcacagct cccacagggt tcgacggcgg tgcggtgacg
     4381 cgctttgcgc cgagcccgac gggggagttg caccttggca atgcgcgcac ggcactattc
     4441 aattttctcc tggcgcatcg cagcggcggg cgttttctgc tgcgtatcga ggacacggac
     4501 gcgcagcgca gccaggaagc gcaccttgcc gtcgtgctgg aagacctgcg ctggctgggg
     4561 ttggactggg acgccggccc ggacaaggag gatgacggtg gaccttaccg ccagtcacag
     4621 cgcggcgcgc tctacgcgcg ctactttcgg gcgctcgagc agcgaaacgc cgtctaccct
     4681 tgcttctgca cccctctcga gctcgaagcc gcgcgccggg cgcagctcgc ctcgggacgt
     4741 ccacccaggt acgccggcac ctgccgggag ctgtcggccg agcagcgccg caacaaggag
     4801 gcccaggggc ttgctgccag cctgcgcttt cgcgttcccg cggggcgtcg catcgagttc
     4861 gtcgatctcg tccatggcgc acagagcttc ctgtcggacg acatcgggga cttcgtgatc
     4921 cgacgcgcag agggcaccgc gtcgtttttc ttctccaatg ccgtcgatga cgcgtgcatg
     4981 ggtgtcaccc aggtattgcg cggcgaagat catctggcca acaccccgcg acagcttctg
     5041 attctcgaag cgctggagct ccgtgcgccg ggctatggcc acgtctccct catcctcggc
     5101 cccgacggcg ccccgctttc caagcgccac ggcgcgacga gcgtccgcga gtatcgcgag
     5161 agcggttatc ggccggaggc gctccgcaat cacctctttc gcctcgggca ctcgagcggt
     5221 gagcacgacc tcctcgatct gccggccatg gcgcgcgcct tcgatccggg acatctcggg
     5281 cgcgcctcgg cgcacttcga tgcgcagcag ctgcacgtgt ggcagaagga ggctgcacat
     5341 cgcctgtcgg tggatgacgc gctcgtctgg ctcgcaccgg tgctgcccgc gggtctcagc
     5401 gagcccgccg cgcgcgcttt cgtcgccgct atcctgccga acgtggtact gccgcaggat
     5461 gcgcagccgt gggcgcggat cgtcttcggt ccgcctccgg agctcgagga tgatgccggg
     5521 gagatcgtga gcaaggcggg cgagcgctac tttgcagccg cggcacaggc cgcggcgcag
     5581 agtggtaatg acctccctgc gatcgtctct gcggtccggg cggcgaccgg gaagaagggc
     5641 gcggagctct acatgccgct gcgtgccgcg ctcacgggcc gcagccacgg gccggagctg
     5701 gcgcctcttt tgaaagccat gcctgccggc acggctcagg ggcggctcgc gcgctttgct
     5761 tagattccct tcacggctca gctgacatga ttcgcatcca caactcactc accggagaga
     5821 agcagccgct cgcgcccgtc acccctggcg aggtccgcat gtatgtgtgc ggcctgacgg
     5881 tgtacgactt catgcacatc gggcatgcgc gcatgatgat tgtgttcgat gtggtcaacc
     5941 ggtatttgcg ccaccgcggg cttcgggtca cgtacgtgcg caacatcacc gacatcgatg
     6001 acaagatcat caggcgggcg gccgagaacg gcgagccgat cggcgccctg acggagcggt
     6061 tcatcgccgc gatgcatcag gactgcgacg agctcggggt tgcgcgcccc gatcacgagc
     6121 cgcgcgccac ccagtatgtg cccgccatca tcgagatggt ggagcagctc gtcgcgcggg
     6181 actacgcgta cgtggcgccg aacggcgatg tcatgtatgc ggtcgcgaaa ttcgacggct
     6241 acggccggct ctcaggcaaa cgcctcgcgg acttgcgcgc cggcgcccgt gtcgagatcg
     6301 atgaagccaa gcgcgacccg ctcgacttcg tgctctggaa gcatgccaag ccgggcgagc
     6361 ccgcatggga ctcgccgtgg gggaaggggc ggccggggtg gcacatcgag tgctcggcca
     6421 tgtcggtggc gctcctcggc tcgcacttcg acatccacgg cggcggcctc gacctcaagt
     6481 tcccgcatca cgagaacgag atcgcgcaga cctgtgccgc taccggcgac cgcttcgccg
     6541 agatctggat gcacaacggc ttcgtcaaca tcgacaacga gaagatgtcg aagtcgctcg
     6601 gcaatttctt caccgtgcgc gaggtgttgg acaaggcggt gcggcacccg caggtgctgc
     6661 gctacttcgt gctctcgagc cattatcgcg ggccgatcaa ttattcgctc gagcagctcg
     6721 agcaggcgga tgcggccctc ggccgcatca tgactgcgct gcgcgacctg ccgcaatctt
     6781 cggcgccgac gtccgaatac ctcggcccct tccgggaagc gatggacgat gatttcaaca
     6841 caccggaggc cgttgcagtc ctgcagaccc tgacccgtga gatcaacacc gcgaagagcg
     6901 ccgggaacga gatgcgcgct gccgccctcg gcgcggagct gcgctcgctg gcgggtgtcc
     6961 tcgggatcac ggtcacgccg aaggagtggt tttcgctggg ggcgaagctc gtgtcccagg
     7021 ggggcgaccg ggtcgctgcg ggcgcgacga ccctcgcgga tgccgagatc gcgcagcgca
     7081 tcgaggcacg cattgccgcg cgcaaggcga agaattgggc ggagtcggac cggatccgcg
     7141 aggagctcgc ggcggccggc gtgattctcg aggacaagcc gggcggcaag acggagtggc
     7201 ggcgcgcctg agaaaacggc ggcgctggga ggcggtgtgg cagccctgag aggccctgag
     7261 cggcggcgag tggcggcgct cagagcggga gtggcgcccc tgagacgtgg cgagtggcgg
     7321 cactgagagc cggcgactga cggccctcag acggccgctg aggggtggcg cctcagcccg
     7381 cctgccggct atctgcccga ctcctggcgg cggtgatagg cctccagggt attgtgcatc
     7441 agcatcgcca cggtcatggg ccccacgcct cccggtactg gagtgatcca gcccgctcgc
     7501 ctccgcgccg actcgaactg cacgtcaccc acgaggtgcc cgttctcgag gcggttcatg
     7561 ccgacatcga tcacgacggc gccgggtttg atccattcgc ctgggatgag cccgggcttg
     7621 ccgacggcga cgaccaggat gtcggcctgc agcacatacg tatcgaggcc ggtcgtgaat
     7681 cgatggcaga cggtcgtggt tgctcccatg aggagtagct cgagggacat cggcctgccg
     7741 acgatgttgg aagcgccgac cacgacggca tgctgcccct tgaatggagc accgacgcgc
     7801 tccaggagcc taatgactcc atagggcgta cagggacgca tcagcggaat gcgttgcgcc
     7861 agccgtccga cgttatacgg atggaagccg tcgacatcct ttgccgggtc gatcttctcg
     7921 atgaccgcgg tctgctggat ctgatccggc agcggcagtt gcaccagaat tccgtcgacg
     7981 ctcgcatccc gattgaggtc gtcgaccagc gcgagcaggg cggcctgcga cgtcgagtcg
     8041 ctcaagtcgt gagccaccga gaggatgccg acctcctcgc acgccttgcg cttgttgcgc
     8101 acatagacct ctgaggccgg atcgtcaccc accttgacca cggcaagggc cgggcgccgg
     8161 ctgccacggg caagggcggc ctcgatcctg ccgcggacct gatttttgac ctcggccgcc
     8221 agctcacggc catcgatcaa ctgcgccgtc atctctggtg ttccacttcc taccctttga
     8281 ggggcgcgca ttctcgcata tgatgccgcg gctgattccg ctgatccggc cggggcatgg
     8341 cgcagtttgg tagcgcatct gctttgggag cagagggtcg ccggttcgag tccggctgcc
     8401 ccgaccacct aaatcaagga cttgtgttct ccgatctcct atcggagtca tcctgctggc
     8461 acccgaaccc gatcactcat gcgcgagacg atgcgggagc atctgcggcg gcgatataga
     8521 ttggcacttg cgggagtcgt catcggggca gtgttgatga tttctgcccc gggcagtgcc
     8581 ttgacgatcg ccctggagat ggcgggagcg gtggttctcg gcagcgcgct cttgattgcc
     8641 gtgcgtaccc gctgcccaaa gtgcaggaga ctattcgaca gccgtgtcat cgcagcgctc
     8701 gtgcggtact cgcaggcgcc accggagcga tgtccccact gcggggttga cctcaaccaa
     8761 tcgcgagcgt gaatagctgg acctcgacct ttgtcattga attgccgaag cagagaaagc
     8821 cttgacaaag acagcctagg caaatatagt gagctccatc tcccatcagg gggattttct
     8881 cggagagcga tcttggcgag catctacgac agcgaaaact acgagccctg caggagcgtg
     8941 gctcacctgg taggccaggt gcggacggag ctgctggcgg ctctggatcg ggagctgacc
     9001 gccgacaagc acgttgccga catcggggtg acctccgcgc agttcatcgt gatggcgcgg
     9061 ctggccgcgg cggagcgcaa gaagtccgcc tcggatctgt gcaaggagat gtcgtacgac
     9121 gctggcgcca tgactcgcat gatcgaccgg ctagagagca agggtctcat tcgccgggcc
     9181 cgctgccctc aggaccggag gctcgtgtac ctcgagatga ccgaccaggg gcgcgcggcg
     9241 tatccgcgtc tgcgggagat ttcgatggcg attcagaacc ggtttctgcg cggcttcagc
     9301 cgcacggata cccgtcagct cgagagcttg ctcgggcgga tgttggagaa cgccgcctga
     9361 ggattgcgaa cggttttttg gcgaggaaaa cctgcctagg catatttagc cacggcagtg
     9421 agatagagag aactgggaga ctgacatgac gacccgcgaa gaaactgcaa agacttcggc
     9481 ctggaagagc ccaagggtgc gctggctcgt gctcgttctc gggggcttcc tcgtgctcgg
     9541 cgccgcctgg ggcgtctact gggatcgcga cctgcgctac tccgtctaca cggatgatgc
     9601 gtacgtgagc ggcaacgtcg tgcagatcac accgcagatc gccggcacgg tggtggcgat
     9661 cggcgccgac aacacgcagt tcgtgaaggc cgggcagccg ctggtgcgcc tggatcccgc
     9721 ggacgccaag gttgccctgc agcagtcgga ggcagggctc gcgaagacgg tgcgccaggt
     9781 ccgcaacctc ttcgcaacca cctcggagct gcaggcgaac gtggaggtcc gccggacgga
     9841 cctcgccaag gcgcagagtg attacgaccg tcgcgcgcgc ctcgagcgca gtggcgcgat
     9901 ttcgctggaa gagctgcagc acgcggggga tgccgtgaag tccgcacagg cggcgttgct
     9961 ggccgcgcag cagcagatgg ctgccgacca ggccttgatc gacggtacga ccatccagag
    10021 caaccctgct gttcgagacg ccgcggcggc cgtgcatgcc gcttacctca actacgcccg
    10081 tacggtgctg ccagcacctg tctccgggtt cgttgcccgc cgcgacgtgc agctcggcga
    10141 gcgcgtcaat accggaacgc cgctgatggc agtggtgccg ctcaacgagg tctgggtcga
    10201 tgccaacttc aaggagtcgc agctgcagtc catgcgcgtc ggccagccgg tctcactgac
    10261 ttccgacctc tacggcagcg gtgtcgtgtt ccatggccac gtggtcggct tcggcgccgg
    10321 gacgggctcg gccttctcgc tgctgccggc tcagaacgct accggcaact ggatcaagat
    10381 cgtgcagcgg gtgccggtgc gcgttgcgct cgatccacag gagctggcac agcatcccct
    10441 gcagatcggg ctctcgatga aggcttacgt caacgtacgt gacgacaacg cccagcggct
    10501 gccccaggtc gcggccaatg accgtgacta ctcgacggac gtcttccagt ccgttgcggc
    10561 ccaggcggat gccacggttc agcagatcat cgcggcgaac tccccgctgg cggggcgctc
    10621 gagcacgggt caccggtttg cggcgcggct gaatcgcgga tcgacactcc tcgccgtgcc
    10681 tgcccgcgcc actctgccgg ttgcgctgag cgaataagct cgcggtccgc ggaagcggtc
    10741 tcacctcagg ggttccaaca tgagcgcagt cattcgggaa cagcaggacg tgccgccgca
    10801 gtcgaggccc gcggcggcgg cacctgcgcc gaagtcggat atgccgtgtc tgaagggctc
    10861 ggctctggcc ctcggcacga tcgcgctttc gctcgcgacc ttcatgaacg tgctcgacac
    10921 gtcgatcgcg aacgtcgcga ttccatccat cgccggcgat cttggggtct cgccggatca
    10981 gggaacgtgg gtgatcacct cgttcgcggt ggccaacgcc atctccatgc cgctcaccgg
    11041 ctggctcacg cgccggattg ggcaggtgcg cctctttacc gcctcgacac tcctcttcgt
    11101 ggtggcctcg tttctctgcg gactcgcgcc ctcgatcgga atgctggtgg ccttccgggc
    11161 cctccaggga ctggtggccg gtcccatgat tccgctctcg caatcgctgc tgctcgcgag
    11221 ctatccgaag gccaaggcgg gcgccgcgct cacggtatgg tcgatgacca cgctcgtggc
    11281 accggtcgtc ggcccggtgc tcggcggttg gatcacggac aacatctcct ggccctggat
    11341 cttctacatc aacgtgccca tcggcatcgc cgccgtcgtc gtgacctggg cgatctatcg
    11401 gcgccgcgag acaccgactg cgcgcctgcc gatcgacaaa gtgggactga cgctgctcat
    11461 cgtctgggtc ggtgcgatgc agatcctgct cgacaagggc aagaacctcg actggttcaa
    11521 ctcgcctttc atcgtgacgc tcgccatcat cgcggtggtc ggcttcgccg tcttcatgat
    11581 ctgggagctg accgacaagc atccgatagt cgacctgagc ctcttccgcg gtcgcaattt
    11641 ctccgtgagc accgtggcga tgctcgtcgg ttacggcctt tacttcggca acgtggtgct
    11701 gctgccgctc tggctgcagc aatacatggg ctacaccgcg acgctcgcag gagtcgtgct
    11761 ggcaccggtc ggagtactgg cgatcctgct catgccggtg gtggggcgct tcctctcccg
    11821 catcgacccg cgttggatcg tcacggtgtc gttcctggtc tttggcctcg tgatgttcca
    11881 gcgctcctgg ttcaacacgc aggcggacat ccgcacactg ctggtgccga ccctcattca
    11941 gggtgtggcc ctggcgacgt tcttcgtccc gctgctcacg ctgggcttct cgggactctc
    12001 cgcggaccga attcctgcag cctcggggct catgaacttc gcgcgcatca ccgcggggtc
    12061 attcgcgaca tccatcacca ccacgctctg ggacaggcgc gcgacgctgc accacgcgca
    12121 gctggtggag cacctgatcg gctccaacgc ctcgatgacg caggccctgg gacagctgca
    12181 cgccgatggc ctcacgagcc agcagggcta tgcgctgctc aacaacctgg tcgacaacca
    12241 ggcgtacatg ctctcggcca acgacatctt ctacgtgtcg ggatttctct tcgtggtgct
    12301 ggcgggcctc gtctggctgg cgcgtccatc gaaggccggt ggggcgggaa aggaagccgc
    12361 tgcgggtgcg cactaacccc gcttgcttgc gcactcggca agtgtctggc gcagctccgc
    12421 gagcttcggc ggcttgctga gaatgtagtc gacctgcggg gcgggctcgc cctcgagcgg
    12481 catgtgcctg ccccagcccg tcaggagcac gatgggcgtg ggtggagcga gcgccttgat
    12541 ggccgccgag actttgcggc cgtcgacgta cggcatgccg agatcggtga ttaccaggtc
    12601 gaacggccgg ccggcgcgaa gcgcagcggt gaacgtatcg atcccgcgct ggccgccatc
    12661 ggcggtcacg acctcatgac catccgcttc cagcgcatcg cgtagcgagc ggatgagtag
    12721 cgggtcatcg tctacgagca gcagccgcat cgcgcgcgct ggagcatctg ccacacgctg
    12781 ctcgtgctcc ccgtcaggag ccggactgga gagcgggaat atcaaccgca cggccgtccc
    12841 ggcgttcggg gtgctctcga tttccagctc cgcgctgtgc cgccgggcca ttccgtagac
    12901 catggcgaga ccgagtcccg tgccccgctc ccccttggtg gtgtagaacg gctcgaggca
    12961 acggcgacgg gtgacctcgt ccatgccgtt accggtatcg cgcacttcta cgagaacgtg
    13021 ctcggcagcc gtgctgtcgc tgcgcgagac gctgttggcc cgcgccgcgg tgcgcaaggt
    13081 gagcgttccg ccctgcggca tggcatccac ggcattgaac acaaggttag tcagggcatc
    13141 gcggatctcg ctgcctacac ccatgatcgc cggtagcttg ctggtaagct cggtacggac
    13201 gtggacgacg acgccgcgct gctgtggcag gtcactccag cgtgcgcggg tcagcgccac
    13261 gacttgctcg acgacttcat tgagctcaac gggggcgagc tgcagagttg actcgcgagg
    13321 gcggtagaat tcgcgcatac gcgcgacggt ggcagcgacg tcttcgatgg cgcgctggat
    13381 gatcgtcatg tattcgcgtg cccgctggct aagcgtcgag tcgtgctcca gcagggaagc
    13441 cgtgtaaagt gcgatgggag agatggcgtt gttgatgtcg tgcgcaatgc cactcgccat
    13501 ctgcccgagc gcccgcaggc gctcctgttg gaccagcctc tcctggctct gtcgcagctc
    13561 ctcatatgcc agccgcagcg cctcgtgcag gtgttggcgc tcgcgctccg ccttgcgacg
    13621 ctcctcgatc tccgccagga gcgcttgatt cgagcgcttg agctcatcga tgccctgctc
    13681 gagctccagc gccagcgcgg cgatctcctg gttgctctgc gtcagctcgt cttgcaagag
    13741 ctgcaccgtg ctccgcagcg cctggacgtc gtgatctgcg ccgtgctcgc tcggtgatgt
    13801 gttcacgatg gcgcagtccg cacgacgaga accgttgcgt cgtcatcgtc acgcgcgagc
    13861 gcaacgagca ggcgctgggc ggcgacggtc gctgtctctt gtgcgatcca gggaaagtca
    13921 ctgaagtccc agcgtgaccg cagcccatca gagtgcatga ccaggatcgt accgggggcg
    13981 aaagaatgct cggcgatcgc aggcgccggc gctctgagcc cgacgacccc gcgccgggga
    14041 atgaggttga gccgctccgg cccgccccat acgcgtagct cgatgtcacc gacactcgcc
    14101 acggtgaagg tccggcggct ggcatccagg agcaccagca tcatcacgac gccccgtgtc
    14161 gcctgacagg ctcgtcccgc cccgcggaac agcgcctcga ggggttgatc atgatgctgc
    14221 tcgacatact ggcgggcagc ctgcgctgcg cgctgggcga actggccgtg ccccaggccg
    14281 tcgatgaccc ctgcgagcgc cttgtccccc cacttacgga tgacgaacgc atcgccattc
    14341 tcgtgcagat gacgataggc tcggctcgcc accccaaagt ccagtgccgg cgctgcgtcc
    14401 gtggctgccc gctgccgcag ccagcgccgg cacacgatgt gcgcgcccgc gctgccggcc
    14461 ggcgagtatt ccaactcgtc catcagccgg ttgacggcgc cgagtcccgt gccgaggcta
    14521 cgtgaggtac tgaatccgtc ggtcagagca cgctcgaagt cgtcgaagcc cggtcccgag
    14581 tcggccgact cgatctggat gcccaggcgc gcctcgcgcg aggccggctc gagggacagg
    14641 gtacccccgc cggcgtgccg gatcaggttg gaggcgagct ccgtcacggc cagcgcgact
    14701 tcctcgcaat cgccagcgga gaaacccacg gatgcgctca gccgccgtgc cgcatcctca
    14761 gcgatcctga tgtcgatagg atccgcgatg cgcatcgtga ccgggcgcgg cgagctcgca
    14821 ccaggctgac tcacggggga caccatttgc gagaggtgac cgtcgtgcct gcgcccggcg
    14881 ccgtacggat ctccagctca tccatcagcc gccgggcagc aggcagcccc aggccgaggc
    14941 ccttgccgct ggagtatccc tgctgcattg cgagctccac gtccgcgatg ccggggcccc
    15001 ggtctatgaa ctcgagctcg ataccgcgcc gcccgccgct ctccaggcga cgccaccgca
    15061 tcacgccgcc gcccccatac ttgtgcacgt tgcgcgcgag ctctgacgct gcggtgacca
    15121 cgcgcgtcac atcggtgagg ccaaaaccga gctccatgac agcttcacgc accgagcgcc
    15181 tcgcggcgac gatatctccc tcgctgcgga tcgagacctc tccctgagca gtgccggtca
    15241 tcgccgcgag cggccggcca ccggccggtc caggagatcc aacgcacgct cgaccgagag
    15301 agccgtctcg atgccaccga gcgtgatgcc gatctgggtc gccgtgaccg cgacggaggg
    15361 ccgcatgcct accaccaccg tccgcccgcc catcagtgaa accatgcttg cggtctccgc
    15421 gacggtgcgg gcaaaaaacg aatccagggt ctccaccgag gagatatcca gcacaacgcc
    15481 cttggcgtcg actcgctgca ttgccgccag cacctcatcc tggagcgcgg aaaccgtggc
    15541 atcgtcaggc tccgagggca ccgtcaccat caaagtcttg cgtacgatga tgatggagat
    15601 cgagttgcga tcgctcatcg gccgtacctc gcacaggctc ttttggggaa agctcagccg
    15661 cccgcccctg cgcgaggagc gacacagagc tcgagcagct cgaatgcggt gcgcagcccc
    15721 gcgctcagcg aagagcgcgt cgagacgttg gagagatcga tccccagatg tacgagggtc
    15781 tgggcgatgg atggccgtac gcccgtgagc accacctccg agccgagcag ccgtaccgca
    15841 gcgatggtct cgatgagatg ttgtgcagtc tgcgtgtcga tcgccggcac gccggtgatg
    15901 tcgagcagcg caacggggga ggctgtctcc atcactttat tgagcaacac gtccatcaac
    15961 tgttgagtcc gctggctgtt gagcgtgccg atgagcggta cgagcaggat tccggcccac
    16021 acctgcacca ccgggaccgc ggccatctcg agaatctcct gcgcctgcct gcggatcttc
    16081 tcctcggact cccggcgagc cgtcaggtcg cgggttactt tgccgaagcc gcgcagcctg
    16141 ccttccgcgt cgcgcagggc cgtgatgatg acgttggccc agaatcgaga gccgtccttg
    16201 cgtacccgcc aaccctcgtc ctcgaagcgc ccctcctgcg aggccacttt gagctccatc
    16261 tcggtcttgc cgttctgcag gtcctccggc ggatagaagc gcgagaagtg ttggccgatg
    16321 atctcgtcgg ccttccagcc cttgaggcgc tcggcggccg cattccacga gagtacgtga
    16381 ccacgcgggt ccagggtgat gatcgcgtag tccctgatgt tgtcgatgag caggcgcacg
    16441 agctcctgcg tctccggcga ggtctggctg gccgcggccc ctggatggcc gccgggccga
    16501 gactcgaact gcgttcccat cagagtgact cccgtacggc tcggatggaa acgaaactta
    16561 ccgcattatt gccggggatg ttggacttgg ttcaaacctc gcccggcccg cgagagaaat
    16621 cggccgtccc gggggcggcg ggcgccggga ccaagcgggc gcgggcgggc taagccttgc
    16681 gcagggcgcg gggcggcaag agggctgtca gggtgccctg gccggcgcgc ggtggccctt
    16741 cgaagcccag acagaggacg gcgcccttct tcagctcgaa accctccggg cgcggatcgc
    16801 cgcgcaggca tagcgccagc agcttgccca gcccgggctg atggccgact actgccacgc
    16861 attcggcgcc agcgccctcg cgcctcagag ccgcgagcac ttcttgcgga gatttccctg
    16921 gcgcgagtgc atcgcacgcc actgcctgcg gccaccgtgc tgcttgcgtg aggatcgccg
    16981 ctgtgtcccg tgcacgcacc aacgggctgg tcaggacccg agccggccgc tgcgcgatgc
    17041 gcctcaggcc cgcagcggcc cgccgcgcct tcttgatacc ctccaccgtc aatggccgtt
    17101 cgttatcgtc gggccagcgc ttcgcgtcgc gctcgaaggc gatggcgtgg cgcacgatga
    17161 gaagctgcat gatcaactcc cgtctgcatc agccgaggcg gatccgtcac gagcgctcgc
    17221 caccggcccg tctgcgatgc ccttgggctt gctgcggatc tgcgcagtgc cgtcgcgaag
    17281 gtcctgcatg cgcgagcgca gtgtcttcca gcgcctgccg cgtaccttgc gccaggactt
    17341 ctcgacttta tcgcccatcc ggatggcatt gcgcgactgc ggctccgcca acctgccgat
    17401 gagaaagagc gtttgcgacc cgaagcccgc cgggggctgt tttgcgagtt gggtgagata
    17461 tcggtggacg gcgtcgccat cgtgttggat gccgagtcgg ctttgcagtt tgcgcagcgc
    17521 accgagcatt tcgcgcgcgg gttttgcgta gctcggagcg acgacttcga gcgcgtagcg
    17581 gagcttcttc gtgtgtatgc ggagcttgtg atattcgccc atcggtgagc caggcctcat
    17641 ctggcgggcg catttgcgca gtttccggta gcgcctgcgg agcagatcag gcacgacggc
    17701 caacgcgcag gcagtgcgcg ctgaggcgac tcgttgggca gctgctcgtg cgagctgatc
    17761 gggcagtgcg tccagccact ggcgcgctgg ctttgcatcc aggactcgca gcatcgtgga
    17821 gtgggcttcg cgcctttcgt ccgcgagctg gccgagcaat gggtcgagcg cacgacggtc
    17881 cgtctcggcg aggctattgc ggaatgtact ggccaccgcg acgcgaatgt ccacgtcccg
    17941 cacagcacct aacgcccgga gcagactctt gagctttggg cggctcctgc gcagggcggc
    18001 cgggaggtag gcaccaaaga gactgaggac ggtatccatc cggcggccgg tgacgcgtag
    18061 cgcatgcagc gcctccggac tctcgccgag cctcgctcct ggctcatggg cgcaccactc
    18121 gtcaacgagg cgctggagcg ctgcgatcgc gaattcgcga gcgccggttg cgacgtccat
    18181 cgcatctggc gcgggcgctg ctgtctcgtc aggctttcgc ccctgcggcg gctcgaggcc
    18241 caccgagcgc aggccggcgg caaacttatt ttccgttgcg ggatggagtc cgcattcggc
    18301 gcgcagccga ttcgcgaggc gctccagcga gccgtgctcg ggaccggccg actcgacctc
    18361 cacgcgcttg aggcgtgtcg agctgcgatg actatcgctg cgtgaaaatt gtgtctcatc
    18421 gagagcgatc tcgcccagct gccgtgaggg atcgcggctc agcactgcga agcgccggcg
    18481 cgaggtatgg acctcaaata gcgtacgcag gggcctcgac ccagtgacgt cccggacgcg
    18541 actgccgacg gggccctccg cccgagccaa ggcttttatg ctgcgcccag gcagtggctc
    18601 ggtgagctcc cgccggtcgg ccagatcgtc acgggccgag cggaggcctt tcaacgtcgc
    18661 ctccaccttg ccgccccgct cctcccgcag ccgcagggcg aatcctgcgc ggaaaatgcg
    18721 ccagtcgggg gtgtcgaggt acgtatcgtg aaggtgcagc gtcggcaggc gcttgatgct
    18781 caagccatcc agggtcgggt gtcgtccaag ccaggtgcgg accagaccca ggtccgtagc
    18841 ggcaagctgc cattcgattt ctcgctcttt gccaggctcc tcccgggcgg tggcgtccat
    18901 gtgcggcccc gggcccggcg gcgcgtgcgg cgcagggggt gcggcggcgg ggccgggggc
    18961 aggggcggga ttgagcctgg gcggcggcat ggaagattcg agaataaacc caaattgacg
    19021 cttcctcgcc cggttccgta tcatgcgcgc ccccgtgcgg cgggtctggc aggctcggtt
    19081 gccggtaacg tggcacgcgt ccgaccggcg cggcatgatg caggcagcga gaggacaccg
    19141 agctgagtgg gcgatgcagt ccgcgtgccc gtagctcagt tggatagagc accggccttc
    19201 taagccggtg gtcgcaggtt cgagtcctgc cgggcacgcc agccgccgaa tggaaacgga
    19261 ggccgatgcg ccgggcttgc ctggatcctg gcggctgctt cgggggctta cctgggcccg
    19321 caagggcacg gggaagggga atggatggcg acaacggtgg acgtagctca gctggtagag
    19381 tcccgggttg tgatcccggt tgtcgcgggt tcgagtcccg tcgtccaccc caccatttac
    19441 aaatgggccg ttagctcagc tggtagagca ggagactctt aatctcttgg tcgtaggttc
    19501 gatccctaca cggcccacca aatttcctta gatatcagtg gattgtgcgg tacaaacgga
    19561 aacggtgcgt gattaggcgg agacttgcca actggcacat ctctcagatg gacgcctgtt
    19621 ggtctcagca ttcaggctga gctgcgttgc tccaaatgtc catttgccag ccacgtagct
    19681 gtgcaggcca ataacccaga tatcgtcgaa gtcgttttcc ggaaggttgt gagcgacagc
    19741 agtaggatgc caacgctcgt tcgaagcgct ctcgttcaag atgataagga cctttccctt
    19801 ggcgtaagcc gcagggcgct cgaatgctca tccgttgccg aacagaaacc gcaatccgag
    19861 actgtagaat tcgggtttcg cgctcgcatg accttcgccc tcgatcacgc gcccatccca
    19921 atgcaagccg ttgtaattgc ggcctgcaaa ggtccgcaca aaagcgattc tgggcgccaa
    19981 gggtgactca tcgcctccgg ctgcgaaaaa tattctgacc cgcaggtcct tgtgggtttt
    20041 ggcaaactct tcttcctgtt tgacgatgaa atgatccgca aactcgatgt ccgggctacc
    20101 ggccaggtat ccccaaaaca gcgaggagtc ggtaaacatc gcatagagcg taaacaaccc
    20161 gccaaaagag tcccccccga gaatgcgccg cgcggggtct gctcggtagt tcgcttcgat
    20221 gagaggaatc aactcggact tcaggaagct caggaatttc ggaccccctc ccgacccctt
    20281 tgcgtcgccc ggtaccggcg tgtagtccat cgcccgcagc gcattgtagt cggggcgctc
    20341 accggaatag gcaatgccaa caaccagtat gtccggcatc gccttgtcgt aaaccagacc
    20401 gccgatcacc gaatcgagca atttgaaatc ccattggccg tcgagcaggt agagaaccgg
    20461 atacttctgc tgtttattct tgtcatagtc gacgggcttg cggacgtaca gatcgtagct
    20521 tcggcccgtg gacgtcgatt tgatggtccg cacttcggag ttgggaattg taactgcggg
    20581 atatgcggcg gaccggacgg gatgactgat tgtgacgaca cacaggcaca agacgagaag
    20641 gtagagactg cgtgcgggcg ctttcataca aaccaccttt tcgagtcgga ccctttactc
    20701 tatcgaacgg ggcgctctgc attgtgaccg aaggtcctat ggctaggggt caggactcga
    20761 gtagatacgc gaccagccga cccaaggtgt cgatgagttg cgccctgggc ggcgttcccg
    20821 gggcccgcag cctcttcgca cgatacctgc gcaccaacgt ccgcacgctg gaaaattggg
    20881 agcagggccg ggccaagcca aacgcgcaag cggcgctgct cattcgcctc gtgcagcgct
    20941 atcccgatac cgtacggcgt ctcgcggaga tctaaacacg tcgcactacg cgccatgctg
    21001 cccgtgtgcg tccagcgttc cgtagcgtcc gagcgcgtcg tgctgtgttg acgctgcgct
    21061 gcacgcagcg cgtgcggacg cggctcaggt tgccggagca gggtcgtgtt gcccggccgg
    21121 gcccggggac gagctacgta aatctcagct taagcgtcgc aattcgcgct cgctcacgaa
    21181 aggcgtgaat tgtccgtggc tccggccgtg gaaccgtctg aactgctaag ggttattcag
    21241 cctaagttac aggtagagga ccgcgcccat gcgcgacatc cagcgattga acacgggaat
    21301 cggtgccctg gatgacattc tccacggcgg attgatcccg gagcggctgt atctggtcga
    21361 cggcaacccc ggatccggca agacgacctt agccctgcaa tttctgctcg agggcgcgcg
    21421 tcgcggcgag aagtgcctgt acgtgacgct ttccgagact cgcaaggagc tcgaggcggg
    21481 cgccgcatcg cacggctggt cgctcgatgc catcgatatc gtcgagctca tccctgacca
    21541 gcagcacgag ctgaatggcg agggcgccct gacgatgatc caaccctcgg atgtggagct
    21601 ctcggagacg atgcggaagg tcctggatgc catcgagcgc agtaatcctg cgcggctcgt
    21661 cctcgattcg ctgtccgaga tgcggctgct ggcgcagagc tcgctgcgct atcggcggca
    21721 gattctggcc ctgaaacagt tcttcgtcgg ccgcaggtgc acggtgctga tgctcgatga
    21781 ccgcacggcc gaaggaccgg acttacagct ccacagcatc gcccatggcg tcatggcctt
    21841 ctacgtgact acaccggtat acgggcaggc gcgtcggcaa ctgcaggtgc tcaagtttcg
    21901 cggcagcgat ttctcgagtg ggttccacga tttcgacatt cggcacggcg gcctggtggt
    21961 atttccacga ctggtggcgg ccgaccatgg gacgcgcttc gatcagtctg tcatcaagag
    22021 cggtgttgtg gctctggacg cactgctcgg cgagggcatc gagcggggca ccagtaccct
    22081 gttggtcggg ccgccgggta gcggcaagtc gacgcttgca ttgcaatacg catgcgccgc
    22141 gacgcagcgc ggtgaccacg cggcgagctt tgtgttcgat gagacgaagg ccgcactcct
    22201 ggcgcgttcg cagggaatag gcctgagcct tcgcgaaggc gccggtcccg gcgagatcgc
    22261 gctgcagcag atcgacccgg tcgagatctc gcccggggag ttcgcagcaa tggtacgcaa
    22321 atgtgtcgag caggatggcg cgcgcgtggt ggtgatcgac agcctgaacg gctacctgaa
    22381 tgccatgccc caggacaact tcctcgtcgc ccagcttcat gagctgctgt cctacctcag
    22441 caaccagggt gtcaccacac tcatggtcgt agctcaaagc ggcatgctcg gtacttccat
    22501 gacctccccg gtggatgcga gctacctcgc ggactcgatc gtaatgttgc gatacttcga
    22561 acacgggggc agagtcaaga aagcgctctc ggtcatgaag aagcgcaccg gcgggcacga
    22621 gggctcgatt cgcgagctgt ggttcgacaa ggaaggggtg cacctcagcg agccgatact
    22681 gggattgcgc ggcgtcttga caggtgtgcc cgtagagctg ggaggcggtg cgggtggggg
    22741 agcggagcgg cccctgcgac cgtaacgcga tctgtgaacg aacagggcgt tctgatatat
    22801 ccgccgagcc gtcgcgacgg tcaggtcacg cgcgaggtgt tgcggcgcgc gagtctcgtc
    22861 tgcaccgtct gccacgaggc gctcgaagtg gcgcacgaga tcgatgcggg ctcgggcgta
    22921 ttggtcatga cagacgctgc gctcgcgcta ccgagtgtcc acatcatcct cacatctcta
    22981 tcgcaacagc ccgcatggtc cgatcttccc gtcgtattgc tttgccagac agatcccgag
    23041 acaccggtgg tggctggtat cctcggtgcg ttcacgaatg tgaccgttct ggacaggccg
    23101 acctccgctc gaacgctcat cagcacggtg caggccgcgt tgcgcggacg tttgcggcaa
    23161 taccagatgc gcgatcagct cgtggcactc agagtcgcca aagagactct gcgcaatgcc
    23221 gatcggcgca aggatgaatt cctcgcgatg cttgcgcacg agctccgcaa tccactcgcc
    23281 ccgattcgca acgccggcga gctgctggcg cgaatgctgc cggacgattc gcaggtgcaa
    23341 accgtggcgg cgatcgtcaa acggcagagc tcgcacctgt cgcgcatggt cgacgatctg
    23401 ctcgacgtgt cccggatcac ccaggggagg ataaacctgg aacacgagcc aatcagtctg
    23461 gcttcggtga tctcacaagc cctggagagc gtcgagccgc tgatgcgcga gaaccgtcac
    23521 aaggtcttcg ttgccggcgg ctatggcccg gcatacgtgc tgggtgacca tgcacgcctg
    23581 gttcagtgcg tcgcaaacat tctgaccaat gcgagcaagt acaccgatcc gggaggcgag
    23641 atccgcgtcg agatgcgcag tgacggcagt caagcggtga tcaccgttac ggacaatggg
    23701 gtcggcatac ctgcggagct cttgccgcaa atcttcgatc tgttcgtgca aagcaaccgc
    23761 tcgctcgatc gctcgcaagg aggactcgga atcggcttgt ccgtggttcg gcgcctcgtg
    23821 gaaatgcacg gcggccgggt cagcgcggca agcgacggcc ccggacgagg cgcgcgcttc
    23881 gagattactc tgccgctgat tgatcgcccg agtgaggtga gggaagagca atcggaacag
    23941 accgccgcgc gccgacgcat cctcatcgtg gatgataacg ccgattcggc ggactctctg
    24001 gcgatgatcc tgaacctttg cgggcacgtg gcagagccag tctacggagc ggcggaggcg
    24061 ctcgaacgcg ccgctgcact cgatcctgag atcgttctac tcgacatcgg cctgccgggc
    24121 atggacggct acgacgtagc caggctgctg cgcagccgcg gcagcagcgc gcgcctcgtg
    24181 gcgctcaccg gttacggtca gccggaggac gttcgacgtg ctcaagatgc gggcttcgat
    24241 acgcatctgg tgaagccggt ggacctcaag cacttgctcc gagacctcgg cagagcgtga
    24301 tgtcccgatc gggaacgccg tcaaacagct ggacggcagg tcctggcagc tcgagcgatc
    24361 agcgtcgtgt aaatccctca catcgatgtg ttcggaattc atcccgaatg acgttggcga
    24421 cttcctcggt gggcagctga tgagccttcg cattcggatg ataaggtcgc tcccagccgc
    24481 attcggagca ccgcgcggtc tggctactcc atacaagttg gcgagttttc tgcggaggtt
    24541 ccgccactgt caaccatgag gtcgtgttcc cgtcatcgtg gcgagccact ttcgtgcctg
    24601 aaggtcggcg atcgcaactt aagggtagcc gtggacgaac ccgaatcgaa tgatgagtca
    24661 gatgatgacg ctgtggtcga ggaatgacca gaggagctgg acgagctcgt catggctctc
    24721 gatcgttacc caccgactgc tgtcgctgtt gctatagcac ctatctcgag ggcttgctgc
    24781 ggctctcctc gacgagggtc agtgcacggc cgacgaggtt cgcgagctac tgcggggaga
    24841 tcgagtcccg gtgggctcca cccgcagcgc cctgtctgag gaataggggc ttcctgggcg
    24901 cgcgcaccgg cggggctccg gccggcgcgc gcccctggga ccgtgcggcg ttgcaattcg
    24961 gtcatcctcg tgcatgcctc ggtggcccgt cctgaacccg cgcagcgggc tccacggctt
    25021 gccgtcgcca cgacgacccg gataggatcg cgtgaatgcg ggtccccatt cttcggcgca
    25081 tcaaaatcga tccctacacc gcccaccaat tcattcaaga ctgcgccgac cagtgcgctc
    25141 acgcatgagc gcccgtagtt tgcgagtctt gaagaatcct ccaccatgac cctggcgcca
    25201 atccgtccgc atccgatcgt catctggttc gggagagtcg gtgacatgat cttgttgagc
    25261 gcgcttctcg gcctgctgca tgagcgatat ggagcgcgat gcttcgtcgt cggcgccggc
    25321 gcctggattt ccgacatcta taagagacac caggatgtcg agcaggtctg gtatctgcgc
    25381 cggtacacgc catttctcgt cgatgccggc tggtggcgcg tgttcggcgc cttgcgtcgc
    25441 catcggtcgg agccgatcta catctgtgag acggatgccc gcaagctcaa acgcatccgg
    25501 tggctgctgg cgctgagccg cacttcgccg gatcgctgcg tgttcatgaa tgaggagctg
    25561 cttgcggccg agagccgggg gcagccgctc gagcattggg tggacagact cctgtccctg
    25621 gggcagcgca cgcctccagc actgagccag accgattttc aggtgcccgc cggcggtgcg
    25681 cgcgccccac gcctggacgt ttcggccgaa gacagggctc gcaccgagga gtggttgcgc
    25741 gcaaaggggt ggtatggccg cccgctggtg ctgatccagc ccggcaatcg gcgcaccatg
    25801 cgcgggaaga aactcaagcg gtctgccgcg gatgacaaag catggccgat agaaacctgg
    25861 gctgcgctgc tgcgccgcat tcatacggct ttgccgaatg ctgtgatcgt gctcgtcggg
    25921 gcaccgaagg aggcgctgct ccttggctgg atcgaggagg cggccgccct gcattcggtg
    25981 gcgacagcca cattgccgct ggccgatcta tttgccctct gcgcgattgc gcacagcatg
    26041 atttccgtgg actccggacc tgctcacgcg gctgccgcag tgggcctgcc actggtggtt
    26101 ctgtttggtg cccattcaca acgcgaatgg ctgccgcgca gtgccggctc gcccgtcgtt
    26161 ggaatcggtg gaccgcccgt ctcccgccgc ctcgatcaga tatccgacga aaccgtgttc
    26221 gaggcctggc tcaccctctc aggagctcag gcgggactcg gttccagggt gggcaggcgg
    26281 acctgacatg aacgatacgc gaatcgaggc catgccgtcg caccggaacc tcacccaaca
    26341 aagcacggca cgctgatgcc ccctgcgcag gacggcttag aggaagaccg ggcgaaaggc
    26401 tcagcggcag gtccggacgg cggctcacgg gagagacttc gcgcgcgggc caaacgccgg
    26461 ctcctgggca ctcactccaa acgactcttt ctccaggtgg gcctgccctg gggcgcggcg
    26521 cttctcgtgg gcctggtctc cgtgctctat gccgactgga gcacggccgc ttccaatgcc
    26581 tttctccgcc tgatcgcagg ccgcccctgg ctcgccttcg tgatcacgcc ggcgctgacc
    26641 gtgctggcgc tgtttctcac ccgacgctgg ttcacgggta gcgagggcag cggcattccg
    26701 caggtcattg ccgcgctcca tgcgccgcgc acggaagaag aggacacgct gctgcggcgc
    26761 ctgttcgggc tgcgtgtgat cgtcgggaag atcggggttt cactcattgg cctgctgggg
    26821 ggcctcacta tcggccgcga agggccgacg gtgcacatcg gcgcggcgat catggcggag
    26881 acccgccgct tctatccgca tcgcaacgcg cagctcgagc gtcgcctcct gctcgccggc
    26941 gcggcagcgg gactctcggc cgcgttcaac acgccgctcg ccggcatcat cttcgccatc
    27001 gaggagctcg cgcgtgattt cgagagccgc accaacggca tcatgatcac cgctgtggtg
    27061 ttctcgggcc tgacctcgct cgcgctcgcg ggcaattatc tctactttgg ccagatcaac
    27121 gtgccggaga acctgggtgg cgcgttcgtg ctgcccgtcg ttctttcggc ggcactatgt
    27181 ggccttttcg gtacggcgtt caatctggca gtgctgcgct ggaaaagctg gatgccggag
    27241 tggctcgtcg cgctgcgcgc gcgctggccg ttgctctatg cactgctcat cgggctgctg
    27301 gtggcggcgc tcggtgtggg gacgcacggc gagacatggg gcagtggcta cgatcaggca
    27361 cgccatctcc tgacgggcac ggtaccgctc tctgaagcct acgggctcac caagtggggc
    27421 gccatggtgg tgacctatat ggccggcata ccaggcgggc tcttctcgcc gacgctctcg
    27481 atcggcgcgg ggattgcgca gtgggtgcac gcggcgttca gctgggcacc gatggcggcg
    27541 gtgattgcgc tctgtatgac gggttacctc gcggcggtca cacaatcgcc gattacgtcc
    27601 ttcgtcatag taatggagat gaccaacggc accggaatgg tcattccaat gatggcgacc
    27661 gcgctcgttg cctcgcgcat ctccgccttc ttcacgccgc ctctctacga ggctctcgcg
    27721 gcgagcaact acttccacgt ggctgctctg ccgacgggaa gcttcctgca gaagaagccg
    27781 gtcccgcctc ccggagccta ggccgcggac tgatgaaatc gactctgctc atcgccgcag
    27841 gtacggtcgc tcttttggca gccgcttcca cggtgcgcgt gatccgcaca ccgactctgg
    27901 ctgctgtcct gcaactctca ggctcggttt tgctggtcat catggtcctg acgcacattg
    27961 ccgaggccta cgcgctggct ccgcaaatgg gttggggacg acccaatacg cctggacact
    28021 acctggacct cgtgagcgct gtcgctggcc ttactctgct cctcgcggga atttccttac
    28081 gagcgatcgg aaccggacgc agggccagct gattctgcgg ctcagtcctc gggactcacc
    28141 tcaaagcgca gcacgtctcg gcggaatcct cgcgtccgca cggcaccgac gatcactccg
    28201 acgatcatcc agctcgcccc gacgagcttc gcggcgttgc tcaggtagat ccacaggatc
    28261 aggcaggtca cgaatcctgc cgcaggtggc cagaagtcgc gcaatcgccg ctcgcgactg
    28321 cgcacgtagt agcgcgccag ggcggccgca ttgaccccca tgtacgcgaa cagcgctccg
    28381 aaattgagta tctcggcgcc gagtccgtag gagatcagaa aggcgccgca gagcgcgatg
    28441 aggcccacga ggatcacgtt gttgcgcgga atgcggtgct tcgggtcgac ggcgccgaag
    28501 aactcaggcg gcagggcatt gctgcgcccc atgccgtaga ggagtctggc cgcgcccaga
    28561 tgcgagccga caccggagcc gatgcccgcg atgagcagcg caagattcat caccacgaag
    28621 agccaggggc cggcagctct ggccgcaacc tccacgaagg ccgtgtcgac gttcctgaag
    28681 ggcagcgccg tgggccacac cagctgcgcg gcatacacct cgatcgccga cagaatgccg
    28741 atcaccaggc acgttccgac ggttgcaaac agaatgttgc ggcggggatc ctcgacctcc
    28801 tctgacagcg tcgagatgcc atcgaagccg atataagtga gcacggcgat cgaggtgccg
    28861 ttgagaaccg cgccggcgcg gaaggtcttg ggatcgtaga acggatgagt gaagaacccc
    28921 ggggagccgg gattgtgatg ccggacgtat aggcccgcgc acacgagaaa caccaggacg
    28981 acaccgccgg tagtcgcggc cagcgcgccc gagatgcgcg cggagctctt gatgccaacc
    29041 aggttcacgg acgtgaacag tgcgaagaat gcgattgccc acactgcgta ggggacgtcc
    29101 ggagcaaaat tcattgctgc ccggctgcac catatgatcg agatgagtgg attgagcaca
    29161 tagtccatga gcattgccca acccgttacg tagcccaaga caggtgtgaa ctcgcgggtc
    29221 acgtacgtga aggccgagcc ggcgctgggg tgcgccgccg ccatgcggcc gtagctgatt
    29281 gccgtaaacg ccatggcaat cattgcgagc aggatggtgg tcgcgacgtg gccattcgcc
    29341 cgcgcgctca gcaccccgaa ggacggcatc ggcgcggtga gctggatcac gatcactcca
    29401 tagaggatca ggtcccaccg gttcagcgcc cgcttcaggc ctgttggctt gtcagtcact
    29461 cgtcggcact ccgcggaccc ctcccgcagc cagtgtggcg gttcaagtaa gatgcagcgc
    29521 gtgctgcgcg ggctcccggt cttgacgcag agctcgcgcc gcagcgcaca cggcgccgat
    29581 gaggcctgca cgatcgccga gcctgctcgg agcgatggtg atgggtcgtg agaagtgggc
    29641 ggggagtgcc agacgcaccc gctcatacag ctcgggacga acgcctatgc tgcccccgaa
    29701 gacgatgagc tccaggtcga ggagcgactg cagcgtggcg acggcgagag ccacccagcg
    29761 tgccgtcgtg tcgagcacca ccgcggccac ctcatcgcct tcctgcgcga gcctgaaaat
    29821 atcacgtacc gtcacggcgg cagcgccgcc ctggcggcgg tatcgctcca cgatggcgag
    29881 cgagcccacc tcgagctcga aggcaccgga agtggccgca gcctgcgaag tcgggtcgcg
    29941 cccgatcggc agatccgcga tctctccggc cgagccgcgc gcgccccgaa acaacttgcc
    30001 gtcgatgaga agcccgagac ctgctcccgt tcccagagcc agaaacgctg cgttgcggca
    30061 ccggctcgcg caaccgagcg tctgctcgcc gagcatcgcg aggttgacat cgttctcgat
    30121 ctcgaccccg ggcccgaagc ggccgcgaag gtaatcgagg acatcgaagt cctgcagccc
    30181 ttcgatgttc ggagtcaggg aaacccggcc cgtgagcgga tcgatcgcac cgggtacgcc
    30241 caccatgaca tgacgcacgc gcgcagcctc gatgccgacg gcgtgcgcca gcttgagggc
    30301 gctggccgcg atctgctcgg ctacgtgtgc gccgccgcgc cggtccgtgg gctccgtcag
    30361 ctccgcgacg atccggccgt gggcatcggc catggcggcg gccagcttgg tcccgccgag
    30421 gtcggctgca aagcaatggg tgctctcgcg agtcatcgac ttgggttact gcacgcgggg
    30481 cggtggcaga ggcagggccg cagcgagatc cgcgacgctg aagagctcat catggagctt
    30541 gttcaaggcg acggacaggg cacccactac cccggcacgc ttgccgagtg tgctggtgcg
    30601 gatctccacc gggcgcggcg cgagccgtaa cagcacgcgc gcgactttgt ccgcaaagtc
    30661 cgctcgggcc cctaccgagc cgccgagaac gatcagttgc gggtccaccg ttgcgatgac
    30721 cgttgccgcc gcgaggccgg tcagccgcgc cgtctcgtca atcacatccg tggcccgcgg
    30781 atcgcctgac tgcgcgcgct cgaatacctc tcgagccgag ccgacgtcgc cgccgccggc
    30841 ttcccgatat cgccgcacga tgccgtgggc gcccacctgg tactcgagac agccctgaag
    30901 gcgcgcttcg gcctggagcg gatcgccgcc gatcggcaga tagccgatct cgcccgcggc
    30961 gccattctcg ccgcggacga gttggccgtt gacgtacagc cccagcccga caccggtgcc
    31021 gagcgcgagg aaagcgacgt tggctacgtt ctgcgcgcac ccatgccaga tttccccgag
    31081 cagcgcgaga ttgatgtcgt tctcgatggc aacggggctg ccgagcttct cgctcagcag
    31141 gcccaccacg ttgagatggc ccaggcccgt gatgttgggc gccatctcga tggcgccgct
    31201 cctgggattc accacacccg gggtggcgag cacgatggag cgaatccggc tcgggtgcgt
    31261 atggttgtca cgtgccaggc ggtcggcgag ccgggcgatc tgctcgagcg cccaggcgcc
    31321 gccgcggctg tcggtgggct catcggtctc ggcgagaacc ctgcccgcga tgtcgcccag
    31381 ggtaaccgtc agccgcgccc ccaggtcgat gccgagcacg tgcccgccgt ccgggctcag
    31441 ctcgtagagc actgcagtgc gtccgacatt gccgctggtt cggccgatgg ggcgaaccag
    31501 cccctgctgc tcgaacgcgt cgatgacctc cgaggtggtc tgcttcgaga gaccggtcac
    31561 cttggcaagg tccgcccgcg agatcgggct gcgatgcaga agggcctcga acacgctccg
    31621 tcgcgacaac tgccgggtca cgctggccat cggctgagaa ggcatctgtg tcctcagttg
    31681 attcgtcata atcacgaact attataccga ggcagttccg taacgccagg aatattgaca
    31741 cgaccatcgc cgtagcgcag tattgattcg tcaggcgacc gaatcaataa gccatgtaac
    31801 gcacccatga acgcggcaat cggccattgc ggtcgacaaa ccggtgtctc gcgatgccat
    31861 ttttgcggcc ggatgtcagt aggacactcg ccccggttgc gttcattcag gtttcgaccg
    31921 ccggggggcg cggggtgaaa cggagctcca ataccgggag gcgtctcgcc gcgtttgccg
    31981 tcgcgtggtt ggcgacgagg ggagttgcaa cagcagcccc tacggcatcc tcctggtcgc
    32041 tgttgcctca gtccgctcag gcgcgcctca cgggatcggg tgtggtgaaa gttgcggatg
    32101 gcgcgcttgt cgcggtgcac ggcgtcgatc gtcaggaagt cgagcccgta gcggatcggt
    32161 tcgtgaagtt ggtggcggtc acccgcgggt tgaagttgcg cgcagcggca gcggctgacg
    32221 cacaccccgc aatctcgttc gaagtggatc ctcacgcgag tgtcgtgggg gacgcaggtt
    32281 accgcatcgt ggtgagcgct ggaggcgtca gagttatcgc gcgctcggcg cggggcgcgt
    32341 tctatggaag cgtcaccctg tggcagctgc tgacgccgcc cggctggatt cgcggcaccc
    32401 cggctgagat tcccgagggc cgcatcgacg atcacccgcg tttcgcatgg cgtgcgctct
    32461 tgctcgattc aggccggcac gtccagagcg tgagcgacat caggaaattg atcgactgga
    32521 tggcgctcga gaagctcaac gtgctgctct ggcacctgac agaggaccag ggctggcgcc
    32581 tcgaaatccc gaagtatccc gcgctcacga aaaccggcgc gtgccgtaag gcggtgggtc
    32641 tcgacatcga attgaccggc ggtgccgaca agccctactg cggcttctat accgaagcgc
    32701 aggtgcggga catcgtgcgc tacgccgccg cccacttcat caccgtcgtc ccgggaatcg
    32761 accttcccgg acactcgcag gcggcagtcg ccgtgtatcc atggctcggc gtaactggta
    32821 agcgtccatc ggtatggacg agctggggcg tcagtccgtg gctgctgaaa cccgacgaga
    32881 ggaccctgca gttcgtcgat gacgtgctcg atgaggtcat gcggctcttc ccctcacaat
    32941 acgtctcgat cggcggcgac gaggcggaca agcagcaatg gaatgcatcg cccgaagtgc
    33001 gcgcacaaat gcggcagttc ggccttgcga acatggatca gctgcagggc tggttcaccg
    33061 gccgggtcgc cgattatctg gtcaagcacg gacgcacgcc cctcggttgg gatgacgagc
    33121 tgctcgcggg cgcgacactc ccggcatcgg aagtcgtgat gtcgtggcat ggaacggata
    33181 acgagcgtgt ggctctcgag gcgatccggc aaggccacga tgtggtcatg acacccgagg
    33241 agtcgctcta cttcgatcac tatcaatcgg agctgcccga cgagtggccg gggcagcctc
    33301 ccgcgataac cctgcggcag gcctatgaca ccgccgttgt cccggacggc gcaaccgccg
    33361 cagaagccag acgtgtcatc ggtgtgcagg cctgcctctg gacggagttc atgccgagct
    33421 tcgtgcgcga ccagcacgcg ttgtttcccc gtatcgcggc gctatcggag ctcgggtggt
    33481 cgccggcgga cgcacatgac tggaatggct tcctgcagcg cctgacagcc gagctggcgc
    33541 gttaccgggc cctcgggatt ggatattccg acacgccgtt cgccgcggcg tctgtagtga
    33601 ctgcaggtcg cgggggcaca ctgcacgtcg tgctttccaa ccaaacgagc ttcggcaaaa
    33661 tcgcttacac cacggacggc tccgcgccaa cctcggcgtc cgcccagtat gtccacccgc
    33721 tggagttttc gtcacgcgcc aaagtaacgc tgcgggcggc aacattcgcc cctgacggct
    33781 tcgaccttgc tgggccgcgt acgcaggcgc tgaatgcctc gactctgctc agccgcgatg
    33841 gcagcgagct tgcgtcctgc tcgaaccagc cgggaatgcg cgtggggggc acccgaaccg
    33901 cagagggctc gacgcccgtt tacaagctgg acgccggcaa catgtgctgg ctctgggagc
    33961 gtgcgccgtt gggaggcgcg aaaagggtca ccctgacggt cgggcgcgtg gcatggcgct
    34021 tcggggacga ggctgcgcaa gccgtcgtcc gcccgaaagc cagcgcggcc ggtgagctcg
    34081 aaatccatgc cgattcctgc aagggacctt tgctcgcccg attgcccctg gcggctgccg
    34141 cgcaggcgac ggggcagtca gatctcagca ctcgagtagc ggcggcgccg ggcaatgacg
    34201 ctcgcaactt gtgcattttc gccactggcg atccgcgcga cggccaatgg gctctggcgc
    34261 gagtggcgtt ctcgaattga cgggacgcgg aagacgcact ccgcgagggg gagcaccgag
    34321 tggaagcggt cctgacaaca tcaatctaga ggtaacgccc gtgtccaaag tacatcgcaa
    34381 gcatcccgtg tcaattgccg tatccatggc gctgctctct gccatgacga catcagccat
    34441 ggcatcgccg cgtgcgggtg ctccagtgga tagcgcgggc gcaaaggctc ccgcagcgca
    34501 ggacgtgccg gcaacgcctg ctgacaagga ggacacgtcc aaggccgcga ccgggacggg
    34561 caaggaaagc gagcagtcgg tcgagctgtc tacagtcacc atcgtcggcg tgcgcgcctc
    34621 gcagatgcgc gcaatcgagc tgaagcgcct ggctccggat atccaggaca gcatctcggc
    34681 ggagagcatc ggccagttgc cggacacgac catcacggac gcgctgcagc gcatcaccgg
    34741 tgtgcagatc gatcgcaacg ccggcgtggg tacccaggtg gacgtgcgcg gcctgcctga
    34801 agtcggcagc atgctgaatg gcgaagtctt catctcgccg gaccagatca catcgcaaca
    34861 gcccaacttc gaaaccctgc cggcgacgct gttcaatcag gtggacgtca tcaagtcgcc
    34921 caccgccagc cagaccgcgg gcggcatcag cggctcgctc aatctacata cctttcgccc
    34981 ctgggatctg ccgcatggat tcacgttcag ctacagcgcc gacggggaac ggggagatgt
    35041 ctcccgcaag aacggccctg aggcaagcgg cctcgttgca ttcaacggcg acggtcgctg
    35101 gggtctgctg gtctccgccg acttctccga taccacacgg tccggtcctg agaatgaagg
    35161 cgcgaacgcc ggctcgagcg agggcctcga ccagtatggg gtcattctcg atggtgagag
    35221 cgccgcgagt gcgggtgcct acaacggttt cgtgggggca tggaacggct tgccgattcc
    35281 ttcgcagatc gtgcagaacc cggacggtag cgtcgatgtg aacggagacg gcaaatccaa
    35341 cggcctgttc atgggaagcc agaacatcgg cctctacgac tcgaccattc aacgcaagcg
    35401 cgcgtccggc aatgcctcgt tccagatgga cctgggagat gggttcacgt tgaccagcga
    35461 ctacttctac acgcatcaga ggcagtacaa ccgcaacgtc ggcctccagt tcaattccac
    35521 cgactggcag ggtgcgacct acgtgccgct gcagtcgatg aataccggcg ctaccgccct
    35581 gagccagtac aatacgccgc cgcccgatcc ggccttctcc ggttcgcaga tctacaccac
    35641 tcaggtgtac gaaaagtggc cgggtgacgt cgaatcgttc tcgcagatcg agaacacggg
    35701 ctccactgcg cagaacgcca atctgcaggt ggacttcaac gatggcggca atttcagagg
    35761 cagcgtgcgc ggcatccgtg agacggcaaa acaggcattc gtggaaacag acctcaacat
    35821 ttcggactcc gatggctgcc tctggcccaa ccagctgatg gacggggtcg atcccaactc
    35881 cgtgccctgc ggcaccttca tctttccgga ccagctcggc ggcaaccgcg tattcaaccc
    35941 gatcggcatc cccgaggact cggttccgat caccgcgaat ttcaacggcc gctatatctc
    36001 gatcggcatg cccgcatcga tggctgccaa tttcgccaac cccaacggct gggcgatgaa
    36061 gacgatcgaa tcctccggcg atttcgacgc caatgccgct atcactgcgc tgcgcttcga
    36121 tggtcactac gaccttcacc acggcttgca cctcgacttc ggcgtgcgca acagcatcca
    36181 caccgccact aactccggat acactctggt cacccccgtg tacgccggca tgggcgccag
    36241 cgacccgaac ggatgcctgg tgcgttacgt cggcgccgat gtgatcctgg ccggtgacgg
    36301 taacggcggc ggctgcaccg cgggcaacgc ccagggctac ttccgcgggg gtccgatatc
    36361 ggcattgcag atgacgaata cgccgccgcc actggccaaa aactggcagg agtacagcaa
    36421 cctcctcggg tcaggaatca acttctgggc agtcaatccc gccgccatgg acaatccgct
    36481 ggcgtactgg aaatcactct atccgcagac cgagacgcag gaggaccccg gaaatacatg
    36541 ggcggtctgg atgaaggagt cgtccgccta tctgcaaacc gacttcggcg aggagttggg
    36601 cgcaatgccc ttgagtggca accttggtgt acgcgtcatc cacacgaacc ttcaggtcac
    36661 gcaacatttg tccggcgcgc cgggattata cggtacggag tccgccgaca tcggcaccca
    36721 ggtcacccgc cgcggttact acgacgtcct gccgagtatc aacctggcac tggacctgac
    36781 ccctcagctg acgctgcgcc tcgccgcatc gaagaacatg cagccgctcg atctcgtgac
    36841 ctggggcggc ggcctggagc tcaactactc gctggtttat ctccccaacg ggaatcagct
    36901 gtaccaggtg gcggcgggca gctcgcaggg caatccgaac ctcaggccat ggcgctccac
    36961 gaactacggt gcctcgctcg agtactacat caaccccgtg agcatggtcg gcctcgaggt
    37021 gttccgcatc aatgtgcaga gcttcatcga gaacggcaac gtgcagaatt gcaccctgcc
    37081 ggacgaggac ggtatcgtgc gcaatcactg catcacgatc accgagccgg tgcagggctc
    37141 tggcaacagc atccagggcg cggagatcga ttaccggcag ggattcacgt tcctgccggg
    37201 gctcctgtcc tatacgggca tggaattcaa cttcacctac gcgccgagca agacgggcca
    37261 gaccgatctc gccgggcacg ccatcccgtt ccaggacaat tcgaccgaat ccgggaatct
    37321 catcctgtgg ttccagagca agcgtttcga ggcccgtgcc gcttacaact accgttccaa
    37381 gcgagcggtg gagaatcagg tgggcagcat cgccggcctg gaagagtacg aggcgccaca
    37441 gagatatctc gatgcctccc tttcgtataa gttcagcaaa tacgccacgg tcttcgtcga
    37501 gggcaccaat ctcaccaacg agtaccagcg gttttacctc gtgtggccca gccaggaagg
    37561 gcatgcgaac ttctccgagc gtatgtacac ggtggggctc cgcgggcagt ggtgatctcg
    37621 agggacgtca tcggagccgg gtgcgcgcgt cacgcgcgct cggcatgccg aatccgcgcg
    37681 ttcttcggcg tgctggtggc actcgcggca ccgccgctcg cctgcgccgc gagcgccttc
    37741 gcgcctaccc gggtggatgg cggcgccggt tcgaccactc ccatagcgca atgggaaatt
    37801 cagtcgagcg cgaaagcgca gcaaggcggc acggaagtct cctccgccgg cttttcaacg
    37861 agcggctggt accccgtgag cggccgtgcg acggtgatga cggggctgct ggaaaacggc
    37921 atttaccaga acgtcttcta cggcgataac ctgcgcgcga tcgaggatcc cgatgccagc
    37981 ggcagcccgt tcgtaattcc ctggtggtat cgcaccgagt tcactctcgt tgcaggatca
    38041 cgagcattac gcacgttgtt gcgcatcaac ggaatcatcg cgagcacgga tgtctgggtg
    38101 aacgggcatc cggtcgccga ccatacgatg atcgccggtg cctatcccgt gcacgagctc
    38161 gacgtgacac gttgggtgca tgcgggcacg aacatcctcg cgctgcgtgt acatccgggc
    38221 aatccgcgga cgagcctgtc gatcggctgg gtcgactgga acccgactcc accggacaac
    38281 aacatgggcc cgtggcgggg cgtggacatc gttcagacgg gcccggtcga gctcctcttt
    38341 ccccaggtga cctcggccct gtcgctgccc gatcttgcgc gtgccgcgct caccgtgagg
    38401 gtgcaggcga agaatctcga tggcgtggcg cacgaggcga cgatcacggg tagggtggcc
    38461 ggcgtatcat tgcagcgaac gatccacctc gctcccggtg aggcgcagac cgtcgccttt
    38521 tcgcccaaaa ccgatgcggg tctcggcctg agtcatccgc aggtctggtg gccgatcggc
    38581 atgggggcgc atccgctcta tcttttgcag atggctgctg ccgttgatgg ggccgcctcc
    38641 gaccgggtga gcgcaacatt cggcatacgc agcgtcagct cctacctcac cgggaagggc
    38701 tatcgcggct tcgccatcaa cggcaaaccg gtcttgattc gcggcggcgg ctgggcgccg
    38761 gacatgttcc tgcgcgatga ccccaaacgc ctggaggccg agttcagtta tgttcgcaac
    38821 ctcgggctca atgccatccg tagtgagggc aagctggagg acgcgtactt ttacgatctc
    38881 gccgaccgga acggaatcat gatcctcccc ggctgggagt gctgcgacaa atgggagtcg
    38941 gcggccaaga ccggcggcgc gccgtgggat gcggcggacg aggcggtggc ggcagcctcg
    39001 accgcgagcg aggcgcgcct gctgcggaac cacccgtccg tgatcggatt tctgatcggc
    39061 agcgacaacg cgccgccggc ggccatcgcc aggatgtacg tcgacatctt gcatgccgaa
    39121 gactggccga tgcccatcat ctcggccgca tcggatcagg cgacggccgc ggcgggaccg
    39181 tcgggaatga agatgacggg gccttacgct tgggttccgc cggcctattg gtacgcccat
    39241 aggctcggcg gcgcgttcgg gttcaactcc gaaaccagcg cgggtgccag cattccgccg
    39301 ctggacgatc tgacccgcat gctttcgcca caggagttgg aggcgctgtg gaagtatccg
    39361 caggtgcggc agtatcacgc ctcggccgcc tggtcgccct tcgcgaccat cgcgccgttc
    39421 gacaccgcgc tggccaggcg ttatggcgca cccaggagcc tcgaggacta cgtcgccaag
    39481 gcccagctcg aaaactacga caacgtgcgc gcacaattcg aggcgtttga cgcgcacatg
    39541 gatgcggcca accgctcgac cggagtgatc tactggatgc tgaacaacgc atggccttcg
    39601 ctgcactggc atctatacga ttatttcctc aatccggccg gcgcctattt cggcgccaaa
    39661 aaggccaacg agccggtgca catccagtat tcatacgaca cgcgcgccat catgctggtc
    39721 aatcacacgc tcaacgctga gcacggcctg caggcgctca tccgcgtacg caacctggac
    39781 ggcagcgaaa gttacacacg gcgtctgcag gacatcgacc tcgccggcaa cagcgcgcgg
    39841 caattgacag cgttgccggc gttatccggc ctgtcacgca cgtacttcat cgagctcgag
    39901 ctcgcttctg ccaaccacca accgatcagc cgcaacgttt actggctctc cacccaaccc
    39961 gacgatctcg actgggcgca ttcgaactgg tatttgacac ccgtatcgga atacgccgac
    40021 ctgaccgcat tgcaagtgct gccggccgcg aagagcgagg ctcgagcgac gttgcggcac
    40081 gaggaagccg acaccattgt cacgatcacg ctgacggtcc cggcatcgtc cagcacggtc
    40141 gcgctgttcc agcacgtttc gatcaggcag tcggcgggcg gcaacctggc actgcccatc
    40201 ctgtggagcg acaacgacgt cactctgtgg gccggcgaat cactcacgtt gaccgcgcat
    40261 tttgtcccgc agggacccgg tacgccgatc gtcgaggtga gcggctggaa cgttccgacc
    40321 cggaccgttc cggtcgccct ggagagcagc gtgggcgcgt cgccggaaag gagtcattga
    40381 agcgcgcgga ccgcaccagc cgcccggaca cccccgccgg gcgctcttat acaatccgca
    40441 gcaccgatcg ataggagagc gctcacatgc ctgccgcccc cgccgtcgcg aagctggccc
    40501 ggaagttcca cttcatctcc gggctgcccc gtgcgggcag cacgctcctg gccgccatcc
    40561 tcaaccagaa cccgcggttt cgcgccggga tgaccagccc gctggcagac atcatggggg
    40621 tggtgatggc cgaggcgagc agcaagaacg acttctcgtt cgacgtatcc gacgaccagc
    40681 gcgttgcgct gctgcgcggc ctggtcgaaa atttttacgc ggcagaaacc gaagccagca
    40741 tcgtgttcga caccagccgg ctgtggtgca gccggatgca actactcggt acgctgttcc
    40801 cggaagcgaa ggtcatcgcg tgcgttcggc agttgtcctg ggtgctggac agcatggaac
    40861 ggctggtgcg caggcacccg gttggcgtca gcaaagtgtt ccggttcgac accaatacca
    40921 cgatgtactc gcgcgtcgag gcgctgaccg atccacgcgg catggtgggg ttctcattcc
    40981 aggccaccaa ggacgcgttc tatggtcaat acgcgccagg ccacttgctg gtgctgacct
    41041 atgaaagcct ggtgcgcaac ccctcggcgg ccatgcgcgc cgtctaccag ttcatcggcg
    41101 agccctggtt cgagcacgac ttcgatcacg tcgcctacaa tgcagacgag ttcgacgcgc
    41161 gcgtcggcat gcccggcctg catacggtgc gcccgaaggt ggagccgatc gagcgcacct
    41221 ccattctgcc ccgcgaaata ttcggccgct tcgtcaacga atcgttctgg atggacccga
    41281 agaacaacgt gagccaggtg cccatcgtgt agcaggagga cggcggcagc gggccgcagg
    41341 cgctcactgc ccagggggtt ggttgcccct cacaggctgc gggccgcgcg gcggcctgat
    41401 gtggttctcg gcgaggaggt gccgcgcttc ggactccgtc agtttgcgca ctgccacgat
    41461 ggggacgcgc tccggctccc aacgcggcac caccagggtg tccatggtgt cgttgcagat
    41521 catgccggtg tggccggtgt cggcaaaccc cagacgctca cgaaacctga gggtcggcac
    41581 gggcgcgaac agtttgatga ggtaaggctg cgaatagagc ggagcgtaga cgatcagctc
    41641 gctgtcgttg agcaccgtcc acgagccgtc gaagtccgac agtcggaagc accccggctg
    41701 gccgggtagg ggagcggcac tccggccggt cgagcccaga tttgcggcaa ccgccgcgcc
    41761 ctgactcgca gggctcgagc ggttcgaggc gcagccccaa aggagcacgg cagtggccgc
    41821 gagcgcggtg agtacgatcc aacgcagtgg ctgagctcgc atgtacgatg tcctgctgac
    41881 gtgtaaaacg ccgtagccgg tcaaaggctc catggcgccg gtctcgagcg cgcagctcaa
    41941 acctccggca cggccaggtg cgcgctaacg gtccgcggca cttgctgcgg tcggcggcgt
    42001 ctcctcctcc tccaggccct cctcgatccg cggctcctcc agatgccatg ggatgaccat
    42061 cacgacgatg cgcgatcctc catagcgcag cagggcggcc tgcaggcgcc gcaggcgatg
    42121 tccgtggagt aaatactgcc accagtgttt cttcaccact tccggcagca gcaccgcgat
    42181 caggcggtta ccctgctcgc gctggatctc gccgatcagt tgcagcaggg gtccggtcat
    42241 cttgcgaaat ggcgccttga cgcgtacgag ctgtggcggg cgcaggccgg cgtttctcgc
    42301 aggctcctcg acatctcgcg cccaacactg gcgcagccca cgctcctcat cgtctgccac
    42361 ggatcctctg aggtccgtga agtgcaccgc gagaatgtcc ggcgagatgc gcagcgcgaa
    42421 ctgcagcgca cgatcggtca gcttgttcca gcccggggtc gtcaccagga ccagtggcgg
    42481 ctctctgctg cgcacgttga gctgcctttc gtcgcgcagc tgcgcatcga gcctgacgta
    42541 gtatttcttg accgtgcgca gcacgaggat ggtggcgggg atggccagca gcgttatcca
    42601 ggcgccctcg ctgaacttcg ccaccacgat gatcaggagc gcgacgatcg tggcagccgc
    42661 acctatcccg ttcgttgcaa gatgggcgcg gtaccgctgg cggactgctg ccgccgtggc
    42721 gcgccggagc tcgcggtgcc aatgccgcac catccctgct tgcgacatcg taaaggtcag
    42781 gaaggcgccg attgcaaaga gagggatgag ccggtccgtg atgccgccga atacgaccag
    42841 cagcacaccg gcggttactg tcaggtagag tatgccgacg gagaatacga ggcgccgccc
    42901 gacgattgcg aacgggcgcg gcaggaagtc gtcctcggcg acgaggcggc agactcgcgg
    42961 gaagtccacg aagctcgtgt tggctgacag gcagaggacg cagagaacgc tgctgagggc
    43021 aatgtagtag agcgccccgt ggccgacgac tgcgctggcc acctgtgaga gcacgctctg
    43081 gtatccgctt tgggtctcat ccatggcagc gatgccgtaa ccgctcacca cgtacgtgat
    43141 ccccgcgagc agcagcccga gggaggcgca gatggctccg agggttcgat gggcgcgctt
    43201 catcaccggc tcgcggaagg cgctcacacc gttgctgaca gcctcgaccc cagtcatggc
    43261 cgtgcacccc gcggcaaaag acctcagcag cacccacgca ctgagcgccg cggttgcatg
    43321 ggacggctgt gggggcgcaa ccagcgggag cggatgtccg cccgaaacga gcgtccgata
    43381 cgctccgagg ccgaggatgc cgaggaagct ggcaaggaag aggtatgttg gaattgcaca
    43441 cgcgcggccg gcatcggccg tgccgcgcaa attcattatc gagaccagaa cgagcgttgc
    43501 gaggcacagg gccagggtgt ggggatgcag tctcggcatt gcagaggtaa gggcgccgac
    43561 gcccgcggag atgccgacgg caacgttgag cacgtagtcg atcatcaggg cggaggccgc
    43621 gatgaggctc gccccggtac ccaggttgag cttggagacc gtgtacgcac cgccattgtt
    43681 gggatatgcg cggatggtct ggcgatagga ggcgtacagg atgacgagca gcaccagaat
    43741 ggggaccatc acccagccga tgtaggtgag accggcgcct ccgagcggca tcaggaccgt
    43801 caaagcggct tccgggccgt acgatgagga gccgaggccg tccaatccca tcgcgggcac
    43861 gccctcgagc gcgccgatct tgcgctcgac gtactcctcg ttggcgagtc ttcggccgag
    43921 gatgagcctg aggagcgaga tcctgatccg tgaggcggga gacacggtcc attaactctg
    43981 cgcccatact gctgttcctg ctctgcacgg gcccagcgca cccatcaaaa cgcgctcggc
    44041 gctcctacag aagaagctct ccgctcacac gaaaaccagg ctgtctacag gagagccgcg
    44101 cgctgcaccg cagtaatcct gcagggcggt cccgtcgaag gacgagcccc tcgcgatcca
    44161 gggctgtcgc agcaggccgg aatcccagaa atgcacaggg gtatccagga ttttcatccg
    44221 ctcggcggcg cccgcgcccc ggctgcgcct gcgtgcgttc ggcgggacgt tccgggtaca
    44281 tggaaagaag ttggcacgtc gtttgctggc aggcatgggg ttacgggaga ttccgggccg
    44341 catgattcag cttactttcc acgatgccgc aggggctccc ggcccggcgc tcgagtcgag
    44401 cgacttcagg atcaccggtg gggttctctg gagccaggtg gagcatggcc tgattgcctg
    44461 ctttgccggc ggcgcgtgga agcaccgcgg cgaatactat cccggcatct ccatggccgg
    44521 cgcctgctgt ctggcgttcg gcatcgcccg cgacgccctc gtctccgagc cgatatccct
    44581 tttttccgtg accggtacca ctttgcgcgc caacggcgtg gcgatcgcag agtacgacga
    44641 gcggcaggat ctatggcagg ggctgattcg gccgatcagt tggcaggcca tgcgcctcgt
    44701 gagcgccgcg gcggcctcgg ctctggcgga tagcagccgg gcggtgacac tgaacccctg
    44761 ggactacccc gggagcgcct ggcccgcggc acgctcttca tcgaaataca ccacatagcg
    44821 ctcgcgcgcg acccggtata gcgggaccag tctgatgtcg gcgggccgcc cggcgccctg
    44881 cgtgtgccag acgagcggct ctgcggcatc aggcttcacc cactcctcga cgggccggcc
    44941 gccgatcgcg agggtagggg cccggaccgg gtccggcacc ttctcccact gatactgatc
    45001 gacagtggcg tagggaatgg gcggtgccat cagggcatcg ccgaggacgc cagccaacac
    45061 gatgggaccc gagaggatgg cgagcttcga agggtgatgg gccatgcgct cgatcctggg
    45121 cggcgtgcgc agctcgatct cgagcacatc cccgtcccgc caatgccgcg tgatatcgag
    45181 gtaggtaccg ggcttacccg tgtcgatcgg cctgccgttg agcttcgcca gcatcgccgg
    45241 cgtcgaccag tgaggatgcc gcagtcgcag ggtggcccgc accggccgcg cgcaggcgat
    45301 ctgcatgcgc acgctgttgg attcgggaaa gcgtgtgtgc tggcggatcg tgagcccctt
    45361 ctcccgccac tgaagctgcg aagcgaggta gagattcacc cagagcgtat cgcgctcgtg
    45421 atagtagacg ccgcgggaat acctggcgtg attctccagc cccgtgccgt cgcagcacca
    45481 gcacgagtcc cacttggagg agaaggtctt gaagtggccc ggctgcatgg aatagtagta
    45541 agtcatcatg ccggtggccg ggtcctggga gccgagaatg tggttgatca gggcccgctc
    45601 gaagaaatcc gccgcctcca tggaggcatt ccagctgaag tagtggctcg tcagcttcag
    45661 catgttgtag gtgcagcagg tctcggccga gccgaccgag agctgctgcc atgcgtcccc
    45721 gatggggaag aaattctcgc ggttgccgtg gccgccgaag acgaaggtgc gctggcggac
    45781 gacgttctgc cagaagaact ccgcgcccgt gcggtaatcg tcgctgccgg tgagctcgta
    45841 ctggcgcgcg ataccgatca cgcgcggaat gtattgattc gagtgcacgc cgtcgagaat
    45901 gtcctgccgg cgcatgagcg gcgcgagctg cgccgcctga ttgaattgct ccgcgagcgt
    45961 gcgatgggcc tcatcgcccg tcatggagta gacatcggcc agcacctcat tcatcccgcc
    46021 ctgctcgctc accagcatcg cctgcatctg cgcaaggtcg agccgccgcg gaaactgcgc
    46081 ggcccattcc gtcatggcga ggagaatgtc ccgcgcctga gcgcggccca tgtggagcca
    46141 tacatcgcgc agccccgcca tcaccttgtg gatcgtgtac cagggagacc acaggccgtt
    46201 gatgtgaaag ctgtagagtc cgtgtgactg gccgaagtcc atctgtccgg ccagcacctg
    46261 cctgaaagcc ggcttgctct cgggaatccc ggcgcaatag ccgcggaagt cggggtcctc
    46321 gcgcccgttg gcttcttggc aggcagcgag gcgcggcagc agatagtcga ccttgcgcgc
    46381 gagccgcgga tcgtgcgtat gggcgtgcat gatcgccgcg gcagagaggt agtggccgca
    46441 gtagtgactc gcaaggccct gactctccca gccgccgagg ctcggcgccc gccgcggcag
    46501 gccggctacc tgctggaaag gagagagcag ccggtcgcaa tcgaagctcg caaggtagtc
    46561 gaggtcccgc gacatcgcgt cccggaacgg gcccggcaac agcgtcacat cggcgaagtc
    46621 gaattcgcgg cagctgagct gcaccgccgc atccaaccgc acaccgcgct gcttcccctg
    46681 cggcacgatc gcgacagcgc gcttgccggc ccatgcggct aggcctgccg ccgccgacaa
    46741 caacacacgg cgtcgcgtgg tgggtgggct cacgatccga tcctcgcaat tctcagacaa
    46801 aagcgcaagc gctgcggcag cgggctgcgt ccgcatcgag gcacgattcc tgcccctgcc
    46861 ggggcacgca ccgcgcacgg cgaagcgcgt tgtgcgatgc actggagggt atcgatatcg
    46921 gtaagcgcgt cgacagcaaa gaaaccgcgc gcaatgcgca cgctcctgcc cgagcgtgct
    46981 ccggcgcgcc aaccctcgag cctcggcggc tgctgagcgg ccagaaagca ttggtgaccg
    47041 gggcgaattc gggcatcggc cgcgcgaccg cgatcgcaat gggacaggcg ggcgccgatg
    47101 tcgtcgtgaa ctacgtgacc ggtgaggatc gcgccgaggc cgtggtcgag gaaatccgcc
    47161 gcggcggtgt acgggcgtac gcccattgtg cagacgtcgc atgcgaggat caggtcaagg
    47221 agatgtttac caggatggtg gcagacctgg gcacgatcga cattctggtg aacaacgccg
    47281 ggcggcagaa ggatgcacag ttcgtcgaaa tgagcctcgc ggattggaat gcggtgatca
    47341 gcgtcaacct caccgggcag ttcctctgtg cccgggaggc gatccgcgag tttctgcgcc
    47401 gcggtgtcgt gccgtcagtc tcctgcgccg caggcaagat catctgcatg agctccgtgc
    47461 acgaggtgat tccgtgggcg ggccatgcca actacgcggc ctccaagggt ggagtcatga
    47521 tgatgatgaa gagcctcgcg caggaggtcg cgcctcatca catccgcgtg aacagcattg
    47581 cgcccggggc ggtgcgtacg ccgatcaaca ctgcggcctg gagcacgccc gaggcgtatc
    47641 ggagcctcat gacgctcgtg ccctacaagc ggatcggaga gccggcggac atcgcccgca
    47701 cggccgtctg gcttgcctcc gatgagtccg attacctcgt cgggtccacc gtcttcgtcg
    47761 atggcggcat gacgctttat ccgggattca ccgaaaacgg ctgatcgctc gatcagccgt
    47821 tcggattcgc gggcaggtgc tttccggcca cgtacgcagt cagccagtcg ctgctcggca
    47881 gacccaccac ggccccccag tggccccgct catcgcggta gagcagggtg ggagtcccaa
    47941 cgccgccgac ggcatccatg gcgctgaggt gcgcctgcag caccccctga agtgccttgt
    48001 cctgcgtccg ggcgggcgct acgccgccgg attcgtgggc cgtatcgaag cggtcctcat
    48061 ccgcgtgcag ggcgccgagc gggttggatg cagagagaat ggtggttgca cgcgcgaggc
    48121 tgtccgcgtg caggaagccg accatcaccc atctgatgtg caaccgtccg gaggcgacca
    48181 gcggctctgc catcttgtag aagtgatagc agaagatgca gttgggatcg gcgaaaacat
    48241 aaagcatggg cgcagccgcg cggccttcgt tgatgagatg gccgccgctc tcgagcttgc
    48301 ccacgaccga gccgtagtcg ggtttcggcg aatagcgctc acggtaatta tcggtgaggt
    48361 cgttgccgtg cggcgagatc aggtggccga cgaagagata gccgtgctcg ccatagacga
    48421 cctgggtgcc ctcctgattg tggacgatgt agcccgtcaa gccgggcacc tcagtgccga
    48481 actgactcac gacctgcagc ttgccggctg cgaccagtga ggccagcatg ggcgggagag
    48541 gaggggatga ggcgtgcgtc gcggccagtg cgggtaatgc gagcagcgag acgcagaagc
    48601 caacgaggca acggcgcagg gacctgggct tcatggaatc cttgatgggg aggatcagtc
    48661 cggcgagttt accccatcgg caggggcggg gcgggaagca gtccccaccc cgcgcgaatg
    48721 cctgaaatcc aggcgctact caggccgtgt gcgggtgtcc ggccgcgtga tcgacgtgat
    48781 cgtcattggg gctgtgcgcc tcgcgtccga agagaatgac atcggacgcc gagatgatcg
    48841 cggcaatgat tgcgctgatg agccccagat atccgctgat gagctgcagc gcgcgcagat
    48901 ccgtccagcc gtagatcgcc tttgcaagga atgtgagtgt gatgccgagc aggaagagct
    48961 gtcgcggcaa ccctgcacgg aacgatccca gccacgtata cgcaaagaat accgtccaca
    49021 cgatccagaa ccagccgaca taactcatgg gcgagggcgc gccggcggcg atggccgcca
    49081 tccccgaatg tcccgccgtg aacaatacgg caaaggcgaa gaacactatc gagtccaggc
    49141 tgcgtccgtg caggtaggcg agaatgccga tgatcgtcag cagcagcgcg aacggcagca
    49201 tgagcgcgct tccggcgctg tatatctggg ggtaccagcc tgctgccgtc atcgacagca
    49261 tccacgatgt cagggcgagc gccatgtagc ccaccgtgga agtggtgacg catcgattgt
    49321 taaggtcagt gacattgata gccatgaaga tacctcttgt tcttgagtga gttgggtgga
    49381 gcacgtctcc atgtcggatt acgagtcgct catcttactc ctgacggtgg gcaacatcgc
    49441 ggattcaaca tccagccggc ccggagctcg ggcggagcgg caggggcggc cagcgggatc
    49501 gtcgccgtgg ccggttgcac gggccggaaa tgagagtggt tcgcattaac gtcccggatg
    49561 cgactcggcc cacggacagg cggctgttgt cttcccacag gaacttccgg gctgctcggg
    49621 cgtagaatgg cgtagccccc gcggaagcct ccccggatcg gaccccggtg catagaaagc
    49681 tcgcgctctg cctcctgctc gtctcgggtt tcctcgccgc cgcgctcacg gactgcggcg
    49741 gaggtgggag cacatccgct tcggcgccac cgccgtcggg ctcctcgccg ccgccgccgc
    49801 cttcggggac gcccccgcag ccaatgacgc atacggacgt cgtgacgtat cgggatgacg
    49861 ccggccgcac aggggagaac ctcaccgagt ccctgctcac ccctacgaat gtcaactcct
    49921 catccttcgg cctgtatcgg gtgctggccg tggacggcaa ggtcgatgct cagccgctct
    49981 accttgcgca gctcccgatc gcgggtggca ctcacaatgt cgtcttggtg gcgaccgagc
    50041 atgactcggt ttatgccatg gaccccgtat cgggtgacac cctgtggcac gtctcgctgc
    50101 tcccatcggg ggagacgccg agcgacgatc gcggctgcaa ccaggtgagt cccgagatcg
    50161 gcgtgacctc gacgccggtc atcgaccgca gcgccgggcc gcacggggcg atattcgtag
    50221 tcgcgatgtc caaggactct ttgtcgaact accaccagcg cctgcacgcg ctcgatgtca
    50281 cgaccggcgc cgagctcttc aacggaccga cctcgatcaa tccgagctat tcgagccccg
    50341 ccggcgggac caagaccttt tcgccgggac aatacgcgga gcgggccgcg ctccttctcg
    50401 agaaccacac gatctacacc agctggacct cgcattgcga cgttcagccg tattccggat
    50461 ggatcgtggg cttcagtgag agcaccctcg cgcagactgc cgtgctcaac gtggcggcgg
    50521 acagcgatgc cggaccctcg atctggatga gcggcgacgg ccttgcggcc gacagctcgg
    50581 gcaacgttta cctggccacg gcgaacggca tgttcgacac gaccctgacc tcaggcggcc
    50641 tgccgcagaa cggcgattac ggcaattcgt tcctgaagct ctcgacggcg ggcggcggcc
    50701 tcgctgtcgc cgactatttc agtccctcca atgaggtggc ggagtccaac gccgaccagg
    50761 atctgggctc gggcggcgtc atgttggtac cggatgtcac ggactccggc ggtgtcgtac
    50821 gccatttgct gttgggcgcc ggcaaggacg gcaatctcta cgtcatcaac cgcgacaaca
    50881 tgggcaagtt caatccgtcg cgcgacatga cctggcagca gctcaacggc atattcccga
    50941 gcactgtttt ctcgacgccc gcctacttca atggaacggt ctattacggc gatgtctcga
    51001 acacgctcaa ggccttcccc gtcaccgccg ccaggctcgc gagctcgccg tcatcggaaa
    51061 ccaccaccat cttcggctac ccgggtacct cgcccgcggt gtccgccaac ggcgcctcga
    51121 atgggatcgt ctgggccgtg gagagcgccc gcactgcggt gctgcacgct tacgatgcgg
    51181 cgaacctcac ccatgagctc tacaacagca gtcaggctgc caacgcgcgg gatcagttcg
    51241 gcgccggcaa caaattcatg acgccgacca tcgccgacgg gatggttttc gtgggcacgc
    51301 ccaacagcgt ggcagtgttc gcgctgaagt gaaggccgcg gtcagccgct tgccgtcgcg
    51361 atgcccggct cgaccgtttc cacgagctcg cgcagcgcac ctgagctgac gagctccctt
    51421 gcggcctcga tatccggcgc gaagaaccgg tcgcggtcgt agcgcggcac ggcgcgccgc
    51481 aggcggcgca gcgctgcctc cagggggcgt gagctggtga gcggacggtg aaactcgatg
    51541 ccctgcgccg ctgcgagcag ctcgatccct atgatgcagg cggcgttcgt cgccatctcg
    51601 agcagccgcc gcgcgccatg ggtcgccatg gagacgtggt cctcctggcc cgccgaggtc
    51661 ggaatgctgt cgacgctcgc cggcgtggcg agcgtcttgt tctcggccac gagcgccgcg
    51721 gcggtgacct gcggcaccat gaaccctgaa ttgacgccgc tctccttgat gagaaaggcg
    51781 ggcaggccgc tcatcttcgg atcgatgagc agcgccgtgc gccgctcgct cagcgagccg
    51841 atctcggcgg ccgccagcgc gagctgatcg gctgcgagcg cgacgggctc ggcgtggaag
    51901 ttgccgccgg agagcacctc gccatcggcg gcgaacacca gcgggttgtc ggtcacggcg
    51961 ttggcttcgc gcagcagggt ctgtccggcg gactcgatga catccagaca cgcacccatc
    52021 acctgcggct ggcagcgcag cgagtagggg tcctgcaccc gctcgcaggt gagatgcgag
    52081 gcccgaatgc ggctgccgcc gagcaggcgt ttcagcgcct tggccacgcg ccgttgaccg
    52141 ggttgccgcc gcaggacgtg gatccgatcg tcgaatggca cgtcgcttcc cttcagcgca
    52201 tccaccgaaa gggcgccgct cacgagcgcc gcgccaaaca cgtcctggat cgcatagagt
    52261 cccgcaaggg cgagagcggt cgacacctgc gtgccgttca gaagcgcgag accctccttg
    52321 ggtgccagct ccaggggctg caggccgatg cgccgcaggc cctcggcggc cgtcatcacg
    52381 cggcccccgg tccggacctg tccgacaccg aggagcgccg cggcaagatg cgccaggggc
    52441 gcaagatcgc cagaggctcc aacggaaccc ttgctcggaa tgcacggata ggtatcggtc
    52501 tcgagcagtc gcgcaagggc gaggagggtc ggcgggcgca cgccggagaa cccctgggcg
    52561 aggctgccga tcttcaggac cagcaccagc cgcaccaccg catcgggtag ccacggaccg
    52621 acccctgcgg aatgtgacag caccagattg cgctgcaact cggcgagctg tccggttgga
    52681 atgcgggtct gtgcgagcag gccgaagccg gtgttgaccc cgtagaccgc cccgccccgc
    52741 gcgatcatgc gatccacggt cgcggccgag cggcgtacgc gcgccatttg cgcccggctc
    52801 agtgtcaccg caacgggcag cgaataggcg gtgcgaagag atgtcagcga gaaggattcc
    52861 gtgcgcaccg tctggcggcg cgccgccgcg cgcgctgcgc tcactgcgcc gctccgcgcc
    52921 gtgtcccgct catgggcaac tgcagtccat gctcggcagc gcaggcgagc gcctcgggat
    52981 agcccgcatc ggcatggcgc atgacgccgg tcgccggatc gttccacagc acgcggcgga
    53041 tgcggcgctc cgcctccttc gtgccgtcgc agacgatcac gacgccggca tgttgggcaa
    53101 accccatgcc gacaccgcct ccatggtgca gcgataccca ggtggcaccg ctcgccgtgt
    53161 tgagcagggc attgagcatc ggccagtcgg acacagcgtc cgatccgtca cgcatgctct
    53221 cggtctctcg gttggggctc gccaccgagc cgccatcgag atggtcccgc ccgatgacga
    53281 tgggcgcgga gagctcgccg gaggcgacca tctggttgaa agccaccccg aggcgatccc
    53341 gctgcccgag acctacccag cagatgcgcg ccggcagccc ctggaaggcg atgcgcgagc
    53401 cggccatgtc gagccattgg tgcaggtgcg ggtcgtcggg gatgagctcc ttcaccttgg
    53461 catcggtgcg ggcgatatcc tgcggatcac cggagagcgc cgcccagcgg aaggggccga
    53521 tgccgcggca gaaaagaggc cggatgtatt ccggaacgaa gccgggcacc tcgaaggcgt
    53581 ccgtgacgcc gttttcgctc gccatctgac gcagattgtt gccgtagtcg aaggtgggaa
    53641 tcccgcgccg gcggaattcg acgagcgcgc gcatgtgcgt cgccatggac gggccggcct
    53701 cggccgcaac gcgctgcgga tcgctctttt gcagcgcctc ccattgctcg acggtccatt
    53761 ccatcggcag gtagccgtgc accgggtcgt gcgcggaagt ctgatcggtc acggcatcgg
    53821 ggcgcacgcc acgccgcagg atctccggta cgagctgcgc ggcgttgccg catacggcga
    53881 ccgagatggg cttcttcgcg cgggtgctct cctcgatcat cgccagcgcc tcatcgagcg
    53941 agcgcgcctg gcggtccaga tagcgggtcg cgaggcgctt gtcgatgcgc gattgccggc
    54001 actcgatcgc gagcagcgag gcgcctgcca tggtagccgc gagcggctgc gcaccgccca
    54061 tgccaccgag tccggcggtg aggatccagc gcccgcgcaa atctccctgg taatgacggc
    54121 gtcccagctc gacgaaggtc tcgtaggtgc cctgcacgat cccctggctc gcgatgtaga
    54181 tccaggagcc ggccgtcatc tgaccaaaca tcatgagtcc cttgcggtcg agctcgttga
    54241 atttctccca gctcgcccag cgcggcacca ggttggagtt ggcgagcagc acccgcggcg
    54301 catccgcatg cgtcgtgaag atgccgacgg gcttgcccga ctgcacgagc agagtctctt
    54361 ccgggccgag gcgggtcagg gcggcgacga tggcatcgaa gcactcccag ttgcgcgcgg
    54421 ccttgccgat tccgccgtag accaccaggt cctcagggcg ctcggccacc tccgggtcga
    54481 ggttgttcat cagcatgcgc agcggcgctt cggtgagcca ggacctggcg gtgaggcggg
    54541 agccgcgcgg tgcgcggatc gtcctgcgcg cggaggggga gagctcggtc atgataaagt
    54601 cacctcgtga aaccgcacgt acggcatgtg atcctcgcga tcatcgtcgc actcattttg
    54661 ttggcggtgg ccactgttgt agcgcgctcc gtcggttagc ccgcaaagcc atgcggctcg
    54721 ccgcgtgagg ccgagctgcg cgcggcagcc actttgcgcc aatatatata tcagtgcgtg
    54781 agtcgcacca gccgcgccac ggcctccgcg ccgacggcgt tgcgctcgcg ggcgagatca
    54841 tacgcggccg cgatgagggc cgagcgcttc gccggggaaa ggtcgaggcc cgcggcccgc
    54901 agttgctcat cgactgcggc catgaccgcc tcgagaagcg gcagatcgag agtcccgtgc
    54961 gacggatgcc gatagggcgg cgcgctctct ctcaccaacg tatcagggtt cgattgcgcc
    55021 tctgcctcgc gcggtccctc tcccgacacg agccattcga tgcgggtctg cgtcacctga
    55081 cagagtgtgc gcaggtccgt gacgttcggc tccgattccc cgcgcaacca tttgcgcacc
    55141 gcaccttccg agcgcgcgat ggccttcgcc aggccgctcg cactgccgtg cgtcttgagg
    55201 acctgccgca ggcgtgcggc aaaccctcca gcctcctgtc tttcgcgccg tccggtgttc
    55261 acgtccgctg agccacgcag ggtgaggttg cagcacaaat gtagtctcaa gtccgctggc
    55321 cgccaagccc atcaatacaa atatattgac gcctcgtatt aatgtgacgt aacgtccctc
    55381 ctatggtgca gaggactttc cggcgtgcct ctccataccg ccatcgcggg actcgccgct
    55441 gcccgccgcg gacgaagcgg aatcgcactg cgcgggccgc ccgggctcgg tccctggggc
    55501 acgcgctctc cggcgcggtc gtgcgcctgc ggcaggtgca gtcggccgtg gccgtggcgg
    55561 ttgctgcatt gcggcggcag aactgcgaac tggatgaaga catcgccacc ctgctgcagc
    55621 gctgcgtctg cgaccgcatc gaagaccagc tccacgagct ggatgccgca cgccatctcc
    55681 tcgccgcgcg cctgcgccgc gtctgatccc gctataattc tcactctcct gcccgctgcc
    55741 ctgcgaaagt ggcggaactg gtagacgcgc tggacttaga atccagtggg gagacccgtg
    55801 agggttcgag tccctccttt cgcattccca gctcagattc cgaggatatt cgttgatgca
    55861 agtttcagtt tccgctactt ccggcctcga gcgacgcatg gaggtcgccg taccggccgc
    55921 ttcagtgacc acggcggtcg agcagcggct gaaggagatt tcccgcactg cgcgtctgaa
    55981 gggctttcgg ccgggcaagg ctccgctgcc cgtcgtgcgc aagcagttcg gagagcaggt
    56041 gcgcgccgag gtcctgggcg atctcatgcg cagctctctc gcggaggcgc tcagtgagca
    56101 gaagctgacg cccgcggctg gcccgcgcat cgagccgatc gcggtcgatc ccgggaacga
    56161 cctcaagttc gccgcgacct tcgaggtgct gccggagatc caggtcaaat ctccggccgc
    56221 tatcgctgtc gagcgcccga ccgcgacggt ggaggagagc gacatcgatg cgatgctcga
    56281 gagcatgcgc cggcagcggc ccgtgtttac ggcagtcgat cgccctgccc gcgataccga
    56341 ccgtgtcacc gttgactaca ccggccgcat cggcggcgag cccttcgagg gtggcgaagg
    56401 caaggatgtg ccgatcatca tcggctcgcg ccagtcccgg ccggagctcg aggaggggct
    56461 caagggcgcg gttgcgggcg agagccgtac cgtggccgtc accttcccgg ccggcacctc
    56521 caacccaact cttgttggaa agacggccga gctcgcgctc accgtgaaga cggtcgagga
    56581 gcagtcgctc ccggccatcg acgaggaatt ttgccgtaac ttcggtgtcg aagagggagg
    56641 aatcgcggcg ctgcgcgcgg aggttcgcaa aagcatggag cgggagctcg cggatgtgat
    56701 ccgcaacaga gtacggaccc aggttctcga tgcactctat gccgagaacc ccatcgatat
    56761 cccgcgagcg ctggttgaag agcaggtcca acagttgcag atcgacgcgg cgcggcgcat
    56821 gggtgtgcgt gaggcctccc agctgccgcc gcggcagccc ttcgaagccc cggcccgcaa
    56881 gcgcgccgcc ctcgggctgc tcatgggcca gatcctcaag acggagagca tcacggtgga
    56941 ccgccagcgt ctccaggccc gggtcgagga gctggcggcg agctatccga atcccgaaga
    57001 ggcgcgccgt gcgtaccagc acaacgcgga ggccctgcgg cagatcgagt ccgtggtgct
    57061 cgaggatcag gtgatcgact ggctcctgga gcgtgccaag gtgaacgaag tgcccatgac
    57121 cttccgggac atcaccggct tcggccagaa gcccgacaac agcgctgagt cgcaatcagg
    57181 ggagaaggat ccatcatgaa cgagcgtgca ctgaatctgg tacccatcgt cgtcgagcag
    57241 accgcccgcg gcgagcgggc gtatgacatc tattcgcgcc tcctcaagga gcgcctgatc
    57301 ttcgtcgtcg gcccgatcga tgactacatg gcgaacatcg tggtagcgca gatgctcttc
    57361 ctggagtcgg agaaccccga gaaggacatc tcgatttaca tcaattcccc gggcggagtc
    57421 gtgagctcgg ggctcgcgat ctacgacacc atgcagttcg tgaagcccga cgtcagcacc
    57481 atctgcatcg gccaggccgc cagcatgggg gcggtgctgc tcgcggccgg ggcggccggc
    57541 aagcgttact gcctgccgca cgcgcgggtg atgatccacc agcccctggg cggctttcag
    57601 ggacaggcgg cggacatgga gatccacgcc aaggagatgc tggagacccg ggaccggttg
    57661 aacaaaatac tggccaagca cacgggccag agcgtcgatc ggatcaagca ggacacggac
    57721 cgtgaccgct tcatgtcctg ggaagaggcc gtccgctacg gcctcatcga ccgggtactg
    57781 gagaagcgtg gcgagattcc gccggatgcc gccggtccca aggctggaaa accggcctga
    57841 gcgccccgaa attgcctctg cgggggtatt ctgctcccgt ccgggcgtcc aaggcctgag
    57901 gagccgcgtg ccgcaaaagt gaaagctgtc atacaagtcc cgttcttaca acgacttagc
    57961 tgtgtcctgg tgctttgcct agcaggcgcg ccccgtgcta tacatgggtc gcatgcctca
    58021 aacgagggcc ttcgatgagt gacgattccc gcggccgcca cgacgacggt aagctcctct
    58081 actgctcctt ctgcgggaag agtcagcacg aggtccgcaa gctcatcgcc ggcccctccg
    58141 tgttcgtctg cgacgaatgc gtggagctgt gcaacgacat cattcgtgag gagctcgagg
    58201 atcgcgccga gcgcgcgcgc gacaagctgc cgaagcccca cgagatcaag aaggtcctcg
    58261 acgaatacgt aatcggccag gagcgcgcca agaaggtgct ctcggtcgcg gtctacaacc
    58321 attacaagcg gttgcagacc cgcaccggcg ccagccgggc gaaggaagac gtcgagatcg
    58381 ccaagagaac atcctgctga tcgggcccac gggctgcggc aagacgctgc tcgcagagac
    58441 gctggcgcgc ctgctgaacg tgcccttcac gatcgccgat gccacgaccc tgacagaggc
    58501 gggctacgtc ggcgaggatg tcgagaacat catccagaag ctgctgcaga agtgcgacta
    58561 cgacgtcgag aaagcgcaga ccggcatcgt ctacatcgac gagatcgaca agatctcgcg
    58621 caagtccgac aacccctcga tcacccgcga cgtctccggc gagggcgtgc agcaggcgct
    58681 gctcaagctc atcgagggca ccatcgcctc ggtgccgccg cagggtggcc gtaagcatcc
    58741 gcagcaggaa ttcctgcagg tcgacacgag caacattctc ttcatctgcg gtggggcctt
    58801 cgcgggcctg gagaagatca ttctcaaccg gacgcagaag agcggcatcg gcttcacctc
    58861 cgatgtgcgc gccgtcgaca tgcgcccgca cggcagcgac ctgttccacg acgtggagcc
    58921 cgaagatctc atcaagtacg gcctgatccc ggagttcgta ggcaggctcc cggtggtggc
    58981 aacgctcgac gagctggatg tggccgcgct catctccatc ctgacggatc cgaagaacgc
    59041 gctcaccaag caataccggc gcctcttcga gatggagggc gcggagctcg agttccggga
    59101 cgatgcgctg aaagcggtcg cccaccgcgc catgcagcgc aagacgggcg cccgtggcct
    59161 gcgcaccata ctcgagacgg tgctgctcga taccatgtac gagctgccct ccatgcgcaa
    59221 cgtcgccaag atcgtaatcg acgagagcgt aatcgaaggt gcgacgcagc cctacgtggt
    59281 gtatcgctcc gatgatgggc gcctcgcgag cgtcgacgag ccccgccgcg ccgtctcaaa
    59341 gtaatccttt ccccttccgc ccatcactac cgttgaaacc cgctggcggt gcccgcattt
    59401 gccgctagaa tgggtttcaa ttcggtgacg gcctcctgcg cacccaagga gccgccgccc
    59461 tcatcgcacc gaggacttga gcgtgtctga acctactgct cccgcccatc cgacgaccgg
    59521 cagcccccag ggggtccctg tgctgccgct acgggacgtc gtcgtctatc cgcacatggt
    59581 gattccgctg ttcgtcgggc gggagaaatc catccaggcg ctcgatgtcg ccatgaaggg
    59641 cgacaagcgc atcatgctgg ttgctcagaa gcaggccgac gtcgatgacc cgaagggaga
    59701 agacctctat cggatcggga ccgtggccac catcctgcag ctgctgaagc tgcccgatgg
    59761 caccgtcaaa gtcctggtgg agggcgtcga tcgcgcccgc atcgacaagc tcaccgctgg
    59821 cgattactac gctgcggagc tcgcgctcct tcccgacgtc gaagcgtacg aggagcggga
    59881 gatggacgtg ctcgcgcgct cagtcatctc gcagttcgag cagtacgtga agctcaacaa
    59941 gaaagtcccg cccgaagtgc tgaccgcgct cgccgggata gagcacgccg gacggctcgc
    60001 cgacacggtg gcggcacaca tgtcgctgaa gctcgccgag aagcagaagg tgctcgagat
    60061 cctcgacatc aggaagcgcc tggagcacat cctggtcgcc atcgagggcg agatggacgt
    60121 gctgcagatc gagaagcgca tccgtggccg cgtcaaggcg cagatggaga agagccagcg
    60181 cgagtactac ctgaacgagc agatgaaggc cattcagaaa gagctcggcg agatggacga
    60241 gggtggcaac gaagtcaccg agctcgagca gaagatcgcc cgcgccggca tgccgaagga
    60301 ggcgcgggag aaggcgacca gtgagctcaa caagctgaag atgatgtcgc ccatgtcggc
    60361 cgaagccacc gtggtgcgca attacatcga ctggctggtc aaggtcccgt ggaagaagcg
    60421 caccaaggtt ctgaaggaca tcacccgcgc cgagcaggtg ctcgatgagg accactacgg
    60481 gctcgagaag gtcaaggagc gcatcgtgga gtatctcgcg gtgcagcagc gcgtcgagaa
    60541 gctcaagggc ccgatcctct gtctggtcgg ccccccgggc gtcggcaaga cttcgctcgg
    60601 ccagagcatt gcgcgcgcga ccaaccgcaa gttcgtgcgc atgtcattgg gcggagtgcg
    60661 cgatgaggcc gagatccgcg gccatcgccg cacctacatc ggctcgctcc ccggcaagat
    60721 cgtgcagaac atgtccaagg ccggcgtgca caacccgctt ttcctgctcg atgaagtcga
    60781 caagatgtcg atggacttcc gcggcgatcc gtcctcggcc ctgctcgagg tcctcgaccc
    60841 ggagcagaac acgaccttca acgaccacta cctcgaggtc gacctcgacc tgtcggaagt
    60901 catgttcgtt tgcaccgcca acagcatgaa catcccggcg ccgctcctcg accgaatgga
    60961 ggtgatccgc ctccccgggt ataccgagga cgagaagatg aacatcggcc ggcgctacct
    61021 ggtgccgaag cagatgaaga gcaacgggct caaggagtcg gagctcaaga tgtccgatgc
    61081 ggtgctcctc gacatcgtgc gttactacac gcgcgaggcc ggcgtacgca atctcgagcg
    61141 cgagatcgcc aagatctgcc gcaaggccgt caagcagatg ctgacgcaca agaaggacga
    61201 gacttcggtg tcggtcacgc cgcgcaacct ggataaattc ctcggcgtac gccgcttccg
    61261 ctatggcaag gccgaggaca tgaaccgcgt gggccaggtc acgggcctcg cctggacgga
    61321 ggtcggcggc gagttgctga ccatcgaggc ggcggtcgtc tccggcaagg gcaagctgca
    61381 gcacaccggc cagctgggcg aggtgatgca ggagtccatc caggcggcca tgacggtggt
    61441 tcgcagccgt gcccaggtgt tggggctcga cggcgagttc taccagaagc atgacgtgca
    61501 cctgcacatc cccgagggcg cgacgccgaa agacggtccg agcgctggta tcggcatgtg
    61561 cacggcgctc gtctcggcgc tcaccaagat cccggtgcgc tccgatgtcg cgatgacggg
    61621 tgagatcacg ctgcgcggcg aggtgttgcc gatcggcggc ctgaaagaga agctgctggc
    61681 cgctcaccgc ggcggcatca agacggtgct gatcccggat gagaacgaga aggacctggt
    61741 cgagatcccc gacaacatca agggcaatct cgagatcaag cccgtcaagt ggatcgacga
    61801 ggtgctggcg ctcgcgctga cgcagcagcc ggtgccggcg gcccagaccg aagccccagc
    61861 cgctcctcct cccggcccca cggctgagga agagaagcgc ggccgccgca acggccgccg
    61921 cctgaaacag gtcgtccccg cccactagcg aaagcatagc gggccagggc cgctccctgg
    61981 cccgcggcga aagatatagg gtctcgcgaa aatcgtctcg acgattttcg ccagtagaat
    62041 gacagggcca gggccgctcc ctggcccgcg ccaataaaac gagggtgctt agctcagctg
    62101 gtagagcgtc gcctttacac ggcgaatgtc ggcggttcga tcccgtcagc acccaccatc
    62161 caaagaggct ccagcggcca cactagacgc gagctggcgc tcccgctgtt cctcccggct
    62221 ccaggatgcg aagcgcatga gcagtgtcag cgacgggcgc tcgatcagcc gttaggtgat
    62281 gagcgccagg atgatcgcgc tgcctgcagc cagagccgcg aaggcaaggg cattccctcc
    62341 atgcatgcga ccccatgcgg ctctgacgag gccgaaggcg aagagatgca cgagatagat
    62401 ggaataagag gcatccccga ggagcagcgg tactctaaaa cggggcagac catactggtg
    62461 ctcccatgac accacggcgc ccacgatggc tatcgaaccc gcgagctggc acagggcgaa
    62521 atgcggcaga tcggcgagga tcagccagaa gcccgcgatc accaacgcgg cgcaaatcca
    62581 tctcgggaca tgcagcgcca gctttccgtc ccgcgcccgc gcgtagccaa ttggcattgg
    62641 cgtcgccgta gcgccagtcg acgatcgcgc cgctcgcacc gcgcaccgca tccagcacgg
    62701 cgatcagatc gaaggggttc tcgaaaacgg cccggtgacg ccgttcgctc tccgcctgcg
    62761 agcggccggg agccgcttgt ccaggttggg aaaggcgacg gtggcggtcc gttgtgcact
    62821 cgtgcgccgg ggaatccgaa tgggtgcttg cagcgtcgtt ctggctcatc cgtctcacgc
    62881 ccttgcgctc gagggcagta taggacaagc ctccgggcgc gaacggtgcg ccagcgcaca
    62941 tccggtctgc ctcagaggag tgccagcagg cgctcgagat tgcactcgcc ctcgatcgta
    63001 tccgcgagcc gatcgagcat ttgctcgcgg cgcgcgccgt gatcgatcgt cagcggccgg
    63061 gtgaggccgg cccagcgcag cagcgcggtg caggcggccg tctcctcgaa gaggccgtgc
    63121 aggtacgtgg ccatgatttg cccgtcggcc gacaccgtgc catcgaccgt gccgtcatcc
    63181 agccgcaccg ccggtcggtg cagggccgct ccctcggtgc gccccgcatg gatctcatag
    63241 ccctttacgc gcgcgccttc cagcagcagg tggcccgaga cattgcacaa tcgcttcccc
    63301 ggcagcagca ccgtggtgag gtcgagccaa ccgaggccgg ggctctcgcc cgctgcgccc
    63361 tcgacgcctt caggatccgc gatccgccgc cccaacatct gcagaccgcc acagattccg
    63421 aggaccttgc cgccgtagcg cagatgtctc tcgaggtacg ccggccagcc gttggcacgc
    63481 agccactcga ggtcggcgcg aacggacttg gagccgggca ggatgacgag atcggctggc
    63541 ggcggcgctg cctcggggcc gatgtaacgc aactccacct gcgggtgccg tgccaatggc
    63601 tcgaagtcgg tatgattgct gatccgcggc aggaccggca ccacgacccg cagcatctcg
    63661 gctgtccgcg caggagccgc agcggcctca cggcttatcc catcctccgc atcgagctgc
    63721 aggccgtgca gatagggaag cacgccgagc acgggtctgc cggtgtggtg ttcgagccag
    63781 tccaatccgg gctgcagcag cgataggtcg ccccggaagc ggttgatcac gaatccgcgg
    63841 atgcgttgcc gctcgctctc gccgaggagt gcgagcgtgc ctaccagatg ggcaaacacg
    63901 ccgccccgat cgatgtcggc gatgagcagg accggacagt cgactgcctc cgcaaatccc
    63961 atgttggcga tgtccccctc gcggaggttg atttccgcag gtgagccggc gccctcgacc
    64021 acgatgacct cgtactgcgc gcgcagcttg tgatacgaag ccagcacggc ttcgcgcgcg
    64081 gagcgcttga attcgtggta gtcccaggcg cccatctcgc cgagcgcctt cccatggatg
    64141 atgacctgac agcgctgatc cgtgctcggc ttgagcaaca cgggattgag atccacggtg
    64201 ggcggaatgc ccgcagcctg cgcctgcagc gcctgagcgc gcccgagctc gccgccatcg
    64261 atgccgacgg cgctgttgag cgccatgttc tgcggcttga acggcgcgac gcgcacgccg
    64321 cgccgccgca gcacgcggca gaggccggca acgagcgtgc tcttgccggc atccgaggtc
    64381 gtgccttgca ccatcagcgt gcgggcgctc acgtcgggtc aaccactcct gggaatctcg
    64441 agcccgcgcg ccacggccgg acgcgcgagg aagcggtcga gcgcgcggct gacgttggcg
    64501 aactcgccga agccgacgag ctcgcctgcc tcgtaatagc cgatcagatt gcgcacccag
    64561 gggaaggtgg cgatgtcggc gatcgtgtac tcctcacccg ccagccacga ccgtgacttc
    64621 agctggccat cgagtacccg cagcaggcgc ttcgcttccg caacataacg gtcacgcggc
    64681 cgcttgtcct catagctcct gcctgcgaat ttgttgaaga agcccacctg cccgaacatc
    64741 ggcccgacgc cgcccatctg gaacatgagc cactggaggg tcaggtaacg gcccctgggg
    64801 tcgcgcggca gcagctgccc ggtcttctcc gcgagataga ccaggatcgc gcccgattcg
    64861 aacagcggca gcggctggcc gcccggaccg tcggggtcga tgatcgccgg aatcttgttg
    64921 ttgggattga gggagaggaa ctccggtgag agctgctcgt gcgcatcgaa gctcacacga
    64981 tgcggctcat acggcagccc cgtctcctcc agcatgatgg agaccttcac tccgttcggc
    65041 gtcggcactg agtagagctg caggcggtcg ggaaatgcgg gcggccacct gccggtgacg
    65101 gggaacgcta acaaatcggg catggacgct ctcacgacaa aaagcaaggc accgtaggtg
    65161 cggaattgtc gcacacgacc gcggcctggc gctcggtccc cgggggatac ggctgcgacg
    65221 ctgcggttac agctccgcga tggcttgcag acgggtgcgc ctgcggcaac agagctcccg
    65281 acaccggtga caagtatccc ggagttgcct gttatgttat aatctacatt cggccgatag
    65341 tcgcacgaag cgtcatcgag gaggtcttcg ttgtactggg cacccggcga ccgtgaaggg
    65401 agcggcggtg gccgagcgag cgcggtagcg gaacgggtgc cggagcaacg gggtttcatc
    65461 ggtcaggcgg aggatcccca aatggcgcaa tccctcgact cgcgccccgg cggcagcgtg
    65521 ccggcggccg cggatctcga gctcgagcgg gcgagctgcg acctgcagca ccggatggcc
    65581 ggcctcgaat ctgccaatgc gcgcctgcaa caggcgctcg atgtctccga ggcgctgcgc
    65641 gccgaggccg agagcgccaa ccgcctgaag gatgagttca tcgccaccgt gagccatgag
    65701 ctgcgcactc ccctcaatac gattcgcctg tggtcgcaca tgctggcaga gggcgggctc
    65761 tgcgccgccg atgcggcaca gggcgcacag gtcctcgagc gctccgccct ggcgcagcag
    65821 cacctgatcg atgatctgct cgacatctca cgcatgtccc gcggccaact gcgattggag
    65881 ctgagaagca cggatctcat ccaggcggtg gcggcggcgg ccgcacagat caagccgctc
    65941 gcgcaggcac ggcagatccg tctggccacc gatctggccg gtggcacggc catggtgcgc
    66001 gccgatgccg atcgaattca acaggttgcc tggaacctgc tcaccaatgc cgtcaaattc
    66061 accccggccg gcgggcgcat cgacgtatgg ctgcatcggg tggacgatcg catggagctc
    66121 gaggtccgtg ataccggcat gggcatcgac gcgcacctcc tgcctcacgt gttcgatcgc
    66181 ttccgccagg gggtggccag cgcacgccgg cagggcgggc tcgggctcgg cctttcaatc
    66241 gccaagcagc ttgcggagct gcatggcggc tcgatttccg ccgagagcgc cggccctgga
    66301 cggggcgcgc tcttcagggt ctgcctgccc ctcagtcgag ccgcgagcgg catccaggag
    66361 agcgtcggcg gaaggtgccg gactgcgcct ccgaacctct cgaagatcga ggtactgatc
    66421 gtcgacgatg agccgtttgc gcgcgaggcg ctggcgcgcc tgctcgagca gaatggcgcc
    66481 agcgtgcggg cggtggggtc ttgtgccgct gcgcgcgagg cgctgacgct gcgccgtccc
    66541 gatgtgctcg tcgccgatat caggatgccc gaggaagatg gctacagcct tctgcgggcg
    66601 ctacgccgtg aggagcagga gcatcacgag cagcgcatcc ccgctcttgc ggtcaccgcc
    66661 ttcgcgcggc tcgaagaccg tcagcgggcg ctcgccgcca gcttcgatga gcacttgccg
    66721 aagccggtgg atcccgatcg gctgctcgcc gcggtggcga tgctcgcccg gcgcccggct
    66781 aaaaacccgt gtggtggaga tacgcgatga cgagcgcggc gacgaggcag tagataccaa
    66841 aaaagtgcca gcgccccgtc tcgagccagc ggctgagcca tttgagcgcc agaagcccgg
    66901 cgagaaacgc gaacagcacg ccgagaatgc tggcggtggc ggccggcagc acgcccgcga
    66961 ggtgatgatg ttgcgcctga tagaggcgcc agatttcgcg cgcatcggcc ggcggtgtga
    67021 gcacgaccgc gagcgcaaag ctgaaatcct cggcgctgct gcgcctcacg cgcgctagca
    67081 ggccggtcga gatggtcgca ccggagcggg aaaagccccg gaacgggaca cagagtccct
    67141 gaatcgcacc gatcgcggaa tactgcgccg gcgtcagttt gggcacggct gcgatatccc
    67201 ggtggcggtt ctcatacagg cccgcgatga gaatgaacgc gccggcggcg gccagcgcga
    67261 ccgcaatcag gtccagccgg ctgaagagct gctcgaccca ggcgtgcgcc tcgtggcgca
    67321 tcagcacttt ctcgatgaat tcgatgatgc cgccgccgac gatgccggtc agaagcgtgg
    67381 cgacgatcac cgcggccgcg aaattccgga actcgggcac ggagctgaaa aacgcccgcc
    67441 gccagcgcgg ccagaaatag acgatcacgg cgagcatcgt gcccgtatgc agcatgacca
    67501 gcagcagcgt catttccggc gcggagggat ccaggcccat cagcttttcc gcgacgatca
    67561 catgtgcgga gctcgagacc gggagaagct ccgcgaggcc ctgaacgatg gcaaggatga
    67621 tcacgtggac catatcgggg tgctcgctgc agatggcgta aagccgcgca ttgtatgttc
    67681 attgagccac cctcggccgc tgaattgatg tgcttttcta ctaagcggcc taccgcgagc
    67741 gttggggcgg cggggcaccg gcgaatccgg tatggttaac ggtttattac cggggggtta
    67801 ccgatgaccg cagccgatcg ccaatcgaaa gcgccacgga tcgcgagtct ctccgctctc
    67861 gccatcgcct gcgcggcggt ggcagccgcc ggactcgccg gcccggcggc ggcggcgcag
    67921 ggtgttgcca cggcggcgga ggtgcccttc ccgcaagggc acgccgcgcc cggcctgcac
    67981 ggcgcgcccg cgagcgccta ctcgcagctt cgatggcgcc tggtcggccc tttccgcggc
    68041 ggctgggcga cgatggcggc cagcgtgccc ggaagtccgg acacgttcta tttctcgggt
    68101 gccggtggtg gcatctggaa gacgatcgac gccggcagaa cctggcagtc cgtaggcgac
    68161 agcctgccgc cggccgtcgg ggcgcttgca gttgcgcctt ccgcgccggc gacgatctac
    68221 gtgggcacgg ggcaggtatc gctgcgttat gatgtcgccg cgggccgcgg tgtcttcaag
    68281 tccaccgacg gggggaagac ctggaagtcg ctcggactcg ccgcgacccg cagtatcggc
    68341 cggatctggg tcgacccaca taacccggac accgtggtgg tggccgcgct cggccatctg
    68401 ttcggtccca atccgcagcg cggcatctac cgatccaccg atggcggcaa gacctgggtc
    68461 cattcgctcg cgatcaatga cgagaccggc gtcgtcgatc tcggcggcga ccctaccaag
    68521 cccaacctgc tgtacgcggc tgcgtggcag acacgggatt acccgtggct cgattacttc
    68581 gagccacacg agggcccggg gagcggcatt tatcgctcca ctgacggtgg cgcgacctgg
    68641 acacggctct ccggcaatgg ttggccgcag ggaccgctcg ggcgcatcgg tatcgctgtc
    68701 acccataccg cccagggggc gaggatctac gcgaccgtcg cttcgaagac ggcgggtggc
    68761 gtgtggcgct ccgacgatgg cggggatcat tgggtccgcg tcaactccga tgcagagacc
    68821 tttggcaatc catacttctc acgtctggaa gccgatccgc acaatccgga cgtcatctac
    68881 tcggcgggcc agtcgatccg ccgctcgagc gatggcggca agacctggac gatcatcaag
    68941 ggcgcgccgg gcggtgatga ctaccatttc ttccggatcg atcccgagca tccggaccac
    69001 tggatcaccg cctctgacca gggggcggtc atcagtgtcg acaatggaaa aacctggagc
    69061 agctggtaca acgagccgac gggccagttc tatcatctcg gcgccgacaa tcgctttcca
    69121 tactggatct acagcggcca gcaggacagc ggcagcatcg gcactgcaag ccgcagcgac
    69181 tatggcgcga tcactttccg cgactggcat cccgtcggcg gcgatgagcg cgattatatg
    69241 ttgccggacc cggacgaccc caatctggtg ttcggcagcg gcatgggcgg caggctctcg
    69301 cgctacgaca ccagcaccgg ccaggtgtca aatgtcacgc cgtggccggt caacagctat
    69361 ggcgccgatc cgaccaaggt gaagtaccgg tatgggtggg tcacaccgat ggctttcacc
    69421 gccgccaaac cgaccgcgct cctttttgga gcccaggtcc tcttccgctc cacggacgaa
    69481 ggtaaccact gggacatcat ctcgcccgac ctcaccggca aagaggacga tgccaaggat
    69541 tgcaacggcc aagtggccgg cgagcaggcg agggcgtgcg gctatggcat catcagcgcc
    69601 atcgcgcctt cgtccaagag tcccgatctg atctgggtgg gcactgacag cggcctcatc
    69661 cagctcaccc gcgacggcgg tcaacactgg cagaacgtga ctccgcgggg gctgcagccc
    69721 tggcagaagg tgagcgccat tgatgcctcc ccgctcgatc cggcgacagc gtacgcgtcc
    69781 gtggacgacc accgttcgga tcatttccga ccgcacgtct ttcgcactca cgactacggc
    69841 aagagctgga cggaggtcga tgcaggcttg ccggcggggg agttcgtgcc ggtcgtgcgc
    69901 gccgacaccg ttcgcagtgg cctcctctat gccggcacgg cggagggcgc ctatgtatcg
    69961 ctcgatgatg gtgatcactg gcaatcgctg cggctcaatc tgcccaaagc gacggtcaac
    70021 gatctgctgg ttcacggtaa cgatctcatt gcggcaacgc aggggcgggc catctgggtg
    70081 ttggatgatc tgacgccgtt gcggcagcta tcggccgccg tgctcggcgc gccggcgcat
    70141 ctctttgcgc cgcagatcgc ctggcgggtt catcccaaca acaacaaaga caccccgctg
    70201 ccgcccgaga cgcccgcagg gcggaatccg ccggccgggg cggtgatcga ctactggctg
    70261 ggcaaggacg cgcacggacc ggtgacgctc gaaatctaca gtttcacggg ccagctcgtg
    70321 cgccgcttct cttccgatga ggcgcccgcg cacatccagg cggaccgcta cttcgatgaa
    70381 gagtggctgc aaccgccgca gaggctgtca gactcgccgg gcgcgcaccg cttcgtctgg
    70441 aatctgcgtt atccgcgtcc acaggcgacc tcctacgatt acagcattgc cgcgatctac
    70501 ggcgaggaca cgcctacctc agtgcagggg ccattcgtac tgccgggtat ctacagcatc
    70561 gtgctgacag ccggtggcga gcactatcgg gcgccgctcg tggtacagct cgatccgagg
    70621 gtgcacacga gcaacgccga cctgcatgcg ctgctcgcct tcagccagtc gctctgtgcg
    70681 gcgctcgagc gcacgagcac tgcgtatgag caagagaagt cggcccacga tcaactggag
    70741 tcgctggcgc agcgattgac tgcggcgcac ggagaccgcg cattgctgcg cgacgtcgag
    70801 aagcttcgcg atgaaacggc agcgcgccgg gacgacaccg acctcggcac gatcagcagc
    70861 cgcatcagcg cactggagtc cgacgccgag tcggcggata gggcgccgac ggcggcggac
    70921 acggagatgt taggcaacga gtctcatgcg ctggatgagg cgctgcgcgc gtggcacgaa
    70981 gaccaggccg caatcgggaa gctgaacgct cgcctgcgcc gcgcccgtct accggcggtc
    71041 agtaccgccg gctgagacta gtaggcaaaa taggggcccg gaccgccctc ggtgttggcc
    71101 ccggcgatcg ccacgtatac gttgcgaccg aagaagaacg gcaggccgaa atcgaaggag
    71161 cagtcggtgg ccgagttcgg gcagtagcct gactcatcgc cggcgctcgc tccgaggtca
    71221 gcgagtgccg tgttgccctg ataggtgctg aagagcatgt tggcattggc gatctggaag
    71281 tggaccgtgg acaccatccc gttgcgcccg gtattggtcg cgctcaggga aagctcggtc
    71341 gtggggcaga accaggtgct gctgctggtg cccgagccgg agctcggaca ggtcgggatc
    71401 gagttgtcgt cgaagtaaaa ggcgttcgag ccggtatcga agtaggagta cgccagcggc
    71461 gtaccgttgt acgtagtcgt gatgtcaccg gtgctcggat tggaagtcag cacggttgcg
    71521 ctgcccaggc cgttgttgcc ctcggtgccg atgccgaagg tcagcgagcc ggtggcagtg
    71581 ggcgccccgc ccgggtccgg gatggcgggc agctggacga tgacgccgtt gttgtcggtc
    71641 tggaaagaga cgacggggtt cgtgacctgc tcgggctcac tcaccgcgac cccgccgcag
    71701 ctgccggttc ccgtcccgtt cgccgggcac gtatagtaga aggtgttggc ggccgtcgat
    71761 gcgcatccgc cgccgcagtc atcgccgaat ggtccgatgc cgagaatgcc gttggcatgg
    71821 aactctgcga ccgtgtcctc ttcaggcccg gtcccggagc aggcggtggg tatctcgttc
    71881 tggaagctcg ggtcaccgat cacctgcatc ggcacattgc tcgccaactc gccggcgatc
    71941 tgcacgtcgg cggtcttcac cggcccccag ctgaagccgt cgatgaactg cgtgcattcc
    72001 accagcgggt tgccgcccgt tgccagctcc tggtgcagcg ccgaggccag ggatggagtc
    72061 agtaccgagg agatgatccg caagccatag gagccggtat cgacctcgac gtgatcgata
    72121 gtctggcagt tggtcgtgct cgctggcgcg caaaccgtca cggtgatgaa gggtgtgtca
    72181 acgtcgtagc cagaggtggc cgtgacatcg gggccagcat ccacggtcat cacgaccaca
    72241 ttcgacgtct ggccgccgcc tgagcccgtg ccgctgccct tgccggtgct tgcggctgag
    72301 ccgaccacgg tacccgagcc gccgcacccg ctgaggagca ggagcgcctg ggtaagacct
    72361 aggagaatga gtttgcgcac cttatccctc gctccgatcg gttcagtgaa ggtccgagat
    72421 ggagagattc tgcggaatga gtgagggcac atacgcgagg ccgaagtact ggcgcatgtg
    72481 tccgccggcg tgcacgatca gcgagggtga atgcacctcc acgtgattgt gcccagggtg
    72541 catgccgcgt gcggtgagct ggctccgtac cgaggccgtg tactggacga aatacgtgcc
    72601 gagcgtctgc tgcagattgg gcattgccgg gccgctccag ctcaccgcga acactacccc
    72661 ggcgggcgac acgtattcgc ggaccagagt ccccgaggcc gtcgtgatct cctgcacgga
    72721 atagccggtt ccggaccggg tacgaagctg ccccttcatg ctggcctggt cggtctgaac
    72781 cgaggcgatc ttgtctccga gggccgcctt tgcgggcacc atgctgccgc aggccagaag
    72841 gagcgcccct gatgcaagcg cggccgacag ggtcgaactg aatactttca tctatctcga
    72901 cctcgaacct ggggataaag cccagcccgg ccgctgggtc agcgtcccgg cacaccacgg
    72961 gttgacgcga gcgccgccat ggccgagccc tccggacgct agcatgccgt agccggtgcc
    73021 gcgactgtcg tggatcacag acccgtcatc gaagccccga attcagggcc attcgcgagg
    73081 tgagtaccga tttggggccc ctccgggctc atggacagcg ggcagggcct cctatagcat
    73141 tcgccccccc agtcggagcg gtagttcagc tggttagaat accggcctgt cacgccgggg
    73201 gtcgcgggtt cgagtcccgt ccgctccgcc agcctctttt ccgcagtggc cgccaaaaag
    73261 ccgccacgag ccgcaagccg atgatcgaca gcggcttttt tgacgcctaa gccggcgtca
    73321 gtgggctttg cgggattggg gctcggggtg cgtccggggg taggggtcta ccttcgatgc
    73381 accgggacct cctggaaatc tcaccccatt tccccgctag cgccgcagct tgcgctggct
    73441 tcctcgacgg catcgatcct acggaaaggg atttcctcgc catcgcagcc ggctcggcgc
    73501 tttccgccgg tagctatctc ctccactcag tggattgtct cggctccacc tgctcccggc
    73561 tggagacgcg gcgtaattcg agccccgacc aagaagcagg gccctcctat gcttaccgat
    73621 gacatcattg ttgccgctca ggcgacggct tcgccacaga agctattcga tgaaaaaggc
    73681 ctctacctcc tggttacccc cggaggaggc cggctgtggc gattcaagta ccgctttccg
    73741 ccacagaccg ccggcaacaa ggagaagctg atctcgctcg ggagttatcc cgaagtgtcc
    73801 ttggcgcaag cgcgagaccg cagagacgcc gctcgtcgcg acatcgccag cggaatcgat
    73861 ccgagtctgc ggaggacttg cgagagactc tgcgtcggca ctgcctttga gtcggtcgcg
    73921 cgggagttca ttggggtcct gcgagcggcg aacattagtc cggagggcac ctcgcgggcc
    73981 gctgcggatc tgattgagag ggcacggatc ccgtctcggc gttcccgccg ccgcgaaccg
    74041 attagcgcag agaccgtgga taccatggag cgacggttgg agctgcacgt ctttccgtat
    74101 atcggtgagc gcgatgtgcg actactgacc gcgcgagagc tgctggaggt cttgcgtcgg
    74161 atcgagtcca gggaaaccta cgatctcgca catcgcgtcc gctcgatctg cagccgcgtc
    74221 ttgcgatacg cccgcgcgac ggggcggcaa tgcgaggacg tagcctcaga cctcgtgggc
    74281 agcctcactc ctgtggagtc ggagcacatg gccgcaattg tcgagccagc gcggatcggc
    74341 gcattgctgc gctcgatcga agcgtattcg ggggagccgc tcacccggct ggcactcaag
    74401 ctgatgccct acatctttcc gcgaccgatc gagatccgga ccatggagtg ggcgcatctg
    74461 cagcttcatg gaacctcacc ggagtggcgc gtgccttggc ggcgtatgaa aatgcgtgcg
    74521 ccacacatcg tcccgctttc acgccaggcg acagatatcc tgcgtgaggt ccgactcctc
    74581 actggcgcgc gacgctgggt gttcccgcaa ctgcgaaatc cggaacggcc gatgtctgga
    74641 tgttgcatta ctgcggcgct gcggacaatg gggtatgccg gcacggagat gagctggcac
    74701 gggtttcggg cgctagcctc gacgcaatta cacgagctcg ggtggaatga cagctggata
    74761 gaggctcagt tgtcgcacgc ggaccgtaac aaggtcaggg gctcatacaa tcacgcgcaa
    74821 tatctgccgc agcgacggac catgatgcag gcttgggcgg accagctgga ttcgcttcgg
    74881 ggtcgtgggg aggccgatgt atcgcaggtg agatggcgac catcggaaac tgcgacggat
    74941 ggatttcaac aggtcgagtc tgagcgcgcc ttatcgtttc aagccgaggc aatagaagcg
    75001 ctgcgagcaa tcatcgctct gtgcccaaag cgttaagagc agtgatgcgt agatataggc
    75061 cagcgtcact catgctcgac gcgagcgatt gcgtacccct cgacatcttc atcgacccac
    75121 ttcctgaagc gcagttgccc gtcctccggc caagcgcgtc tgagcgttgc gtcaacgatg
    75181 atctcgttcc tactcgtggc cgccgccgtg atcgcgggaa gcataatctc ccgctgatcg
    75241 gacattcagt cgttgtcacg tgagcggttt atcaggctga ccactgtgct caaggctgcg
    75301 ccgacggcga aggcgatcaa cagggccttt atgggctcgt tcctggcgta gttggcggcg
    75361 ctgtcgaagg cgctccgcgc gcccgcagtt agccgccggg cgcgggcgcg aaccgaatct
    75421 acgccacgcc ggacgcgctc gctagcattg gcgtggatgg gccgcgcctc cggatcagag
    75481 ggagagcggc cgttgtcaac agaagatgta ttggtatcca tcggagattc ctcacaaaag
    75541 aaaatatgag agcgaaattc ggaagatcgt caccccaacg aaacgtgaaa cacgcgcggt
    75601 acgcgacgaa cagagcaagt tgggcagcgg aagcggctca gccgcacgcc gctttaccct
    75661 cactcgcact aatgcagatg aagagtaccg ctgccaaaca gtcctagcag tccgataatg
    75721 atcagataga tcgcgacgat gtagttcagc aatcgcggca ccaggaggat caagatgcct
    75781 gcaaccaggg acaccagggg tccaattgtg agatggagat tcatggaaag ctttcatagt
    75841 gaaatgcggg gcggcagatc gggcggcaga cagctgtcac ctcagcgatc tgactccctt
    75901 ttagacagca tgctaaatgg caaaagatcc gtctgtacgc ccgcgcacac gcaacgccac
    75961 tatcctcggc tccccaaccg tgcctgctgg acgggcgtac gagagcctcg agctacatcc
    76021 gcatgggcga agcgaaacgc cagtgcaggg gtcttgagaa gcgggcgagg cgccctgggg
    76081 ccgcctcatg ccgcgatgtg cggacgagga gcaccccccc gatcccgcat gcggcgcgcg
    76141 gcggcagaca cgccgccagc gcccatccca agcgctgatc tcgcctctcg agcccggtcc
    76201 gcggtcacga cctcatcgca tctagcagcg cggagatctc gatcgacgcg aaattggaga
    76261 acgtgtcgca aaccgcatag ggcagaatga tgaggtcgcc gtggcgcatg gctccgcacg
    76321 tatagacgac attcgggacg taaccttcac gctcggacaa ctcggggcgc accagcggct
    76381 ccggcgagcg ggcgagaacc ttggacgggt cctgcttgtc gaggagcacc gcgccgatgg
    76441 aatatcttct catcggaccg acgccgtggg tcagcaatag ccaaccctcg tcgagctcga
    76501 cgggggagcc gcaattgccg atctggacga actcccaggg gaattgcggc ctcaggatgg
    76561 cgatgccctc gtgccaggag tacagatcgt ccgaatagat gagatagagg ttctcattgt
    76621 cctgccgccc gatcatggcg tattggccgt tgagccttct cgggaagagg gccatcccct
    76681 tgttgcgcgc agccgagccc gtcaggggtg tcatcttgaa gcgctcgaag tcggtagttt
    76741 ccatcaactc cgacctgatc gccctaccgt tgtaggcggt gtacgtcgca tagaaagtct
    76801 ttcgaccgcc atcgtcgaac tcgacgaatc gggcatcctc gatcccgttc gattgcgctt
    76861 cggtgaccgg aaagatcacg cgctcgctga tgctgctatc gggctcgaag gcgacttcga
    76921 agcaatcctc cagcgcgcca ggaactcgtc gctgcaggtg tggaatcgac gcaagttgcg
    76981 cgatcggctc gagcctcacg gtcccatcgg cagcaagaac accggaccgg aacgtcagcg
    77041 aagacacatg accttccccg acggcccgca ggctcaggat gaagcgtcgc tcgccacccg
    77101 gcacaccgga ctgatcgggg tgggcgacga tgctcggatt gaacagggcc gcggcttcga
    77161 aggaatactc gtgcaggaag tatgcgccga tgagctggcg ttgcgcatcg gtgagggaag
    77221 tgtgcgtgga gtgagcgagt tccagggtgt cggtacgtgt cgcgaaagtc gccaaaagcc
    77281 gccgatgacg gccctgaaag ctttccacga cgtccgccaa ctgctggcgc acggtctgcg
    77341 ggtcgagctc caagacccgc tgtacgatgt ggctcattcg cctcatgtcc gtcgggtgga
    77401 ggtctcgcgg gtcggtgctc ggcctgaagt cgcgcaccac gactctcgtc ggatcggggc
    77461 gaagatagag ggtctgacgc tgcaccaatg tggcttgaga caaggcatct ccggatggcg
    77521 ggcatgtcat gcgaggctgt caggcgctgg cggcgagctg cagagggcgt ttcgtctgct
    77581 cgccactgag gtggccgagc tgacgcatct cggcgaaact gaggaggtag gagacaacgg
    77641 attcgccacc gcggttctcg ttcgcgcgat ccgggtgcag tccatcgcgg cagctgccgg
    77701 tttccgggtc gatcagcgac aaggacaaat cattgctgcc gcggaaccat gcgaaagcgc
    77761 gcgctgcgtg ggcctgccaa gtgacatcgc tggcgactcg ccaggcggcg aggcatgcgg
    77821 agatcgatgc ggtggcctcc aagggctgct ggtcgaaggc cagcgggcgc gtccgcttgg
    77881 cgccgaagct tttcgagccg acgggtctga agagccccgc cggcgttgtc tgcaggctca
    77941 tgagccagtg cagggctcgt aaaccggcgc tgaggtaatt ggatgcccgg gttgcggacc
    78001 ccgtcacgat caaggcctgg gggaggcgcg cgttgtcata ggcgaggcct tcctcgaacc
    78061 atgcccaatc cggggtctcg accgccgtca gactggcgat caatttgtcg gcgaggaccc
    78121 cgcgcaggcg ccgcgcggtt gcgtcctgcg caacggcggc acagtaggca tccaagccga
    78181 gcaacgtgaa gctccacgcg cgcggcgagc aaaagcgctc cactgccggc agcgcctcgg
    78241 cgaacaggga tgcagcccat tgccgccgtg attgctcgcg atcgctctgc gcgcactcgc
    78301 ccaatgccca gagcgcgcgc ccgtggcagt cctccgatcc cgactcgccg tgccagcgtc
    78361 gatcgaagtc catgaagttg cgaaaccgcc tcgtgtcgcg attccaggcg tgctggagga
    78421 acgcggcgaa ccggctcgtc acggtatcgg gcagcggggt ttctccgggc ttgccgagag
    78481 cgcaggcgag cagcagcgcg cgggcattat cgtcggtgca atagccgtgc gaccgatccg
    78541 gcacgcaatg cgtggcgtgc tggagcaggc cggtatcatc gcagagagac aggaagtgcc
    78601 cgatcggcat ggctcgggaa gcctcctggt cgcggggcgg tccgcccgcg tcctgccgcg
    78661 cagtgatctg gcgcaggtca cttctcctgg cgctcgcgaa gaccgcacgg tagtgctcgg
    78721 cgattctcgg ccaaatcatc gacctcccga ctgcgtaggc gcgctcgcgc attgccaatc
    78781 gccgccgctc gtcactcaac agggccgcgg tctcgcgccc gagggccgcg gcatcaccga
    78841 agggaacgag gatcccccgt ccctcgccga gcaattcccg tgcgtgccag tagggcgtgg
    78901 agacgatcgc cttacccaag ccgaagctat aagccagggt cccggatgtc atctgcgcct
    78961 cattgagata aggggtgaca tagacatcac acatggagat gaaccgcagc agcgttgcct
    79021 ggtcgacgaa ttgatcgaga aagacgacat ggtcctcgat tccgagctct cgtacccgcc
    79081 tcacgagccc gtcccggtag gcctcgccct gatcacggat caaattcggg tgggtggctc
    79141 cgagcaccac atagacggcc gcggcgctgg ttctcaggat ctcgggcatc gcatcaatca
    79201 tgacttcgat gcccttgctg ggggagagca ggccgaaggt cagaatgatc ggtcggccgc
    79261 cgaagccgag ggcggccttc gcttcgtcgg gttccaagaa cgcgcaatcg ggagtcccgt
    79321 gcgcaatgac ttcgatcttc cccaccgcca ctcgatacac ggtacgcagc agttccctgc
    79381 ctttttcggc catgacaatg acgcgcgagg aagtgtcgac gatttctccg agaacgcggc
    79441 gctgcgccga tgtgggctgc gacagtacgg tatggagcgt cgtgacgagc ggcatggtca
    79501 gccgcgaggt aagcgccagg acgtggccgc ccgcctcgcc accaaagatg ccgaattcat
    79561 gctgaagaga taccacctcg caccgtgcca tattcagggc gtgcgccgcc tgctcgtaat
    79621 cctcgagatt gcggtcgttg atctgaagcc ggaccgccgg cgggtagtcg taggtatggt
    79681 cagggtccgt cattgcgacg atgcaggatg actcatgtgg ccgcaactgc tcgatcgcct
    79741 gctgcagatc agtcgtgaag gtggcgatgc cgcagcggcg cgggagcgaa ttcccgacga
    79801 acgcgagccg cccgagtgga ctcaacgtgt ctctccggca ggatgcggcc caagcacgga
    79861 catcgctttg gctctaaagt gcgctcgctg ccggtcgcgg gccatgtcca gcgcgtgctc
    79921 cccgctcaga ccgcggagca ggtccggtgc tgaaacggcg ccagcatagg caacgaggag
    79981 ctcgagacca tccgatccga catcgacgcg cgttccgtca gcctcgagaa agacggcgct
    80041 tccggtttgc acatcgatgg cgcccgccca ggcgcgaccc gccaggatga acaaccacat
    80101 ctctcccgtc gcattcagat tccagacgga ttcaggaggg agcgtcagcc gctcgagcac
    80161 gaagtgcggg ctggcagcca gcaatgtcct tgcggccgtc agctttcgct cagggggctg
    80221 ccgttcggcc cgatcggcgt tcgcggcggc aacggcggca tcaacgtgca gctctcgctt
    80281 cctgccgtag tcaaagagcc ggaacgtcgt atcacttctc tgctgaatct ctacgatgac
    80341 gagtcccgcc ccgatggcat gaatcgtccc cgggggtacc aaagtggcgt cacccgtgcg
    80401 cactgtgtgc cattgaacct gttcggcaat cgagccatcg ccaatggacg ttcggagctg
    80461 cgcagcggtc agccgatgct tcaatcccag ggctatctgc gcgccctcgg tggcggagag
    80521 gacataccac gcctcacact tgccgtgcgc cagaccgatc gagcgcgcgt actcatcatc
    80581 cggatgcact tgaatcgaga gcggcgcgct cgtgaagatc agcttgagga gcaacgcagg
    80641 gcagggagcc tgcgcatcgc tgcgctcgaa ccaaacctct cccatcgtag cggcggcatc
    80701 gtgatggacg ttccagggcc gcaggtccgt gcgaccccag ggcttgggta cgaggcgaac
    80761 tcgggcctgc tcgactgcca tgaaggtctc caggttgacg acattggcgc tgggcacatg
    80821 atgtcgaatg gcgttcagcg ctgcgcccat cgttggctcc tcaccgccga gcagagcagg
    80881 cgcccgctcc atactcctca acactgtacg cgggagtgaa gactcattga cgacacccgc
    80941 ccgagcgagc cccagcgaat cagacgcgac ggtcacgaaa gccgcgaaac atagtgtggt
    81001 agacgagcag gaccactgca gctccgatga tcgcgacaac cgtactgtag atgttgagcc
    81061 cgctcacctc cggcattccg aagagccgga acagccatcc accgacgatc gcgccaacga
    81121 ttccaagcag aatgtcgacg atgacgccct ccccggtgcg gttgacgatc ttgctggcaa
    81181 tgaatccggc aatgagtcca agaacgatcc aagaaaacaa tgacatgttg atctccggca
    81241 actacgcgag tcgaacgagg cccagggagc ggcgagcatc tcgcgatcgg cttcctagca
    81301 taatcgcgaa tcactgcggg ttcggtgcgg cggcgaacat aggcggaatt gccgtcagcg
    81361 ccgacggact accagcctga gcgatcgtgc atgtctcggg cggtccggag cagtgccggc
    81421 cgctcagtcc tttccatgac agagtacact cgacgtgtcg aaatcaccgc agatgttcgg
    81481 tgtcgtgacg tgcgtgacgg ccgcggtgtc cggcggcgct ctttgggctg cggcttgcca
    81541 tacccggcgg gaccgtggcg tgcgcgagct cgcgcagttg ggcctcagtt aggcccgcgc
    81601 cacccgcgag cgtggcgcgc tttggatcga cgcactccgt ccggaccgtc gcctcaatcc
    81661 ctgacgactt tgatctcgct atggactctc ttcacatggg cggttgagcg cgcaatcgat
    81721 acggccttgt ctgccgctcg ctgggttttg acgctcccgc tcagccacac gacaccgtcc
    81781 ttgtcggtat tgacatgaat gtctcccaag ctggttatgt gctccgctgc cagccgagtc
    81841 ttgatttttg tcgtgattgc agaatccttc acgtatgttt ccgcatgcgc ggcatccgaa
    81901 tgagagtcct gggcggtcgc tgctatcgag ccgccgagca aggtggcgac gatcgcgcag
    81961 cctgttgcga ggtaggtttt catgactctt ccatattggg gaccggcact gactatgcca
    82021 ctcctctgca gcggctttcc gttcgatacc gtacgcactc ggtgcccgga agctgttgcg
    82081 g
//
LOCUS       DASRHN010000239         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2385219, whole genome shotgun sequence.
ACCESSION   DASRHN010000239 DASRHN010000000
VERSION     DASRHN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385219"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..2727)
                     /locus_tag="VFB37_09560"
     CDS             complement(115..2727)
                     /locus_tag="VFB37_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO22734.1"
                     /translation="MESHGLRVLVLEDVRVEADLAIQQLARGGIRCVYSRADSEASFR
                     AALSRFRPHLILSDFGLPGFDGMTALQIAVESVPEVPFIFLSGTIGEERAIEALRRGA
                     VDYVLKSNPGRLVPAVRRALKEVSERAGRRLAESRIRQSEQRLRDIVDTSQDWIWEMD
                     AGGEFVFSSESVRGALGISVQDVLGARFERLIHEDDRAVFGGTLARLSAAQKIASGIV
                     TRWRHASGQHRWLECNLTALIGESGQVCGYRGTQRDISERKHQQEMILRLTRMLQMQS
                     GINAAVVRIGEREALLREVCRVALQVGGYELVVVSLVEADGRHVVPWYRLGLDSPSLE
                     SERFPIGDGSEADTSLMGRALRTGEITVCTDLTRSEPPVAHRASLLALGFKSLVALPF
                     SVEGARIGVLSLASRESDQLREEELLLLQDMIANLSFALQYRQKESAARYLAYFDSLT
                     GLPKRAVFCEHLDRLLRKLVAGAGEPIVVAFDVMHLSNVNDTFGRHIGDLLLQRVAER
                     LKQQINDEGRLGYLGGGTFVLVLAQPESSRENVSTLLENTVFREAFHIEGHSIRTSFR
                     SGIARHPLDGDNASTLAQRAEAALKQAKEAGEPYLHYQMHVHSELAERLALEHRLRSA
                     LDEGQFVLHYQPLVDLVTGRIDSAEALLRWNDPEGGLTLPGRFLPVLESSGLIVAVGD
                     WVLKTAARDCQRWNQAGFGPLRVAVNVAAQQIRRRTFVQHCLAAAAGCEAGGGLDLEI
                     TETGLLHDVEGASRKLHDLRSAGMRVAIDDFGTGYSSLGLLSKLPVDLLKIDRTFISG
                     LPSDPASVTLVTSIIGLASAFDLIAVAEGVETKEQADLLRQLKCNHSQGYLHSRPVPA
                     VEFERLLARSSSTL"
     gene            complement(2842..>4133)
                     /locus_tag="VFB37_09565"
     CDS             complement(2842..>4133)
                     /locus_tag="VFB37_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZO22735.1"
                     /translation="GYEVDVAANAAQARASRARQTPDLVLLDIWMPDVDGISLLREWS
                     VSATDGCPVVMMSGHGTVETAVEATRLGAFDFVEKPLSLAKLLRTVERALDAGKRRRQ
                     SGKFIVPALMVPVGKSKQMQQLRGELQQIALNPSTVLLIGEPGSGREAFARYLHERGP
                     RGSRPFVTLIASSLREADAEARLFGREGETSGKQIGLLEEAADGTLFIHEIEDLPPAA
                     QRLLVGVLESGQFTRLGGTGPVQLTARVLSSAQPGVEHRAGAEGLRRDLLAHLNTLIV
                     RVPPLRDYAEDVPELLRHYVDRLVDTEGLQFRRFSVAAQNRLRNYPWPDNVRELKNLV
                     QRLLIQGGAEEIRLEEIERELAVQAPAGEPLVKQDLLALPLREAREQFERAYLQQQLL
                     LCNGKVGQLAKRVGMERTHLYRKLRSLGVDFRNISED"
BASE COUNT          742 a         1412 c         1299 g          680 t
ORIGIN      
        1 tgagctgaac cggttacgca agcagctcca ggccgcagac ctcgacgccc tggtgatccg
       61 ggtcggggac taacccaact ggtgtgggat ggaagcttgg ggtcgccccc cgggtcagag
      121 cgttgatgat gaacgcgcca acagccgctc gaactccacg gccggcaccg gccgtgagtg
      181 caggtagccc tgcgagtgat tgcacttgag ctgccgcaga agatccgcct gctctttcgt
      241 ttcgacgccc tcggcgaccg cgatcagatc aaaggcggat gcgaggccga tgatgctggt
      301 gacgagcgtg acgctcgcgg gatccgacgg caggccactg atgaacgtcc gatcgatctt
      361 cagcaggtcc accggcagtt tcgagagcag gccgagcgag gaatagccgg tgccgaagtc
      421 gtcgatggcg actcgcatgc ccgcggatcg caggtcatgc aatttgcggc tcgcgccctc
      481 cacatcgtgc aacaggcccg tttcggtgat ctccaggtcg aggccgcccc cggcctcgca
      541 accggcagcc gcggccaggc aatgttgcac gaacgtgcgg cgccgaatct gctgcgcagc
      601 gacgttcacc gctacacgca gcggcccgaa tccggcctgg ttccagcgtt ggcaatcgcg
      661 ggccgccgtt ttcaacaccc agtctccgac cgccacgatc agcccggatg attcgagaac
      721 cggcaggaat cgacccggca gagtcaaccc gccctctggg tcgttccagc gcagcagcgc
      781 ttcggcgctg tcgatacgac cggtcacgag gtcgacgagc ggctggtaat gcaggacgaa
      841 ttgaccctca tccagcgcgc ttcgcagccg atgctcgaga gcgaggcgct cggccagctc
      901 gctgtgcacg tgcatctgat aatgcaggta cggctcgccc gcctccttcg cctgcttcag
      961 cgcggcctcg gctcgctgcg ccagagtact ggcgttgtcc ccatcgagcg gatggcgcgc
     1021 aatgccggaa cggaaagagg ttcggatcga atggccctcg atatggaagg cctcgcgaaa
     1081 aacggtgttc tccagaagtg tgctgacatt ctccctggag gattcgggct gggcgagcac
     1141 gaggacgaac gtgcccccgc cgaggtagcc gagccggcct tcgtcgttga tctgttgttt
     1201 cagtctctcg gcaacccgct gcagcagcag atcaccgatg tggcggccga aggtatcgtt
     1261 gacattgctc aggtgcatga catcgaaggc aacgacgatg ggctcgccgg ccccggcaac
     1321 gagcttgcgc agcagcctgt ccagatgctc acagaagact gcccgcttcg gcagcccggt
     1381 gaggctgtcg aaataggcga gataccgggc cgcactctcc ttctggcggt actgcagcgc
     1441 gaaggacaga ttcgcaatca tgtcctgcag gagcagcagc tcctcctcac gaagctgatc
     1501 cgactcccgc gaagccagcg acagcactcc gatgcgcgct ccttccacgc tgaaaggcaa
     1561 tgccacgagc gacttgaagc cgagcgccag caggctggcg cggtgagcca cgggcggctc
     1621 ggagcgggtg agatccgtgc agacggtaat ctcgcccgtg cgcaaagcac gccccatcaa
     1681 gctcgtatcg gcctccgatc cgtcgccgat cggaaatcgc tcggattcca gggatggaga
     1741 atcgaggccg agccgatacc agggcaccac gtggcggccg tccgcctcca cgagagagac
     1801 gacgacgagc tcgtagcccc cgacctgcaa ggcgacccgg cagacctcgc gcagcagggc
     1861 ctctcgttcg ccgatgcgca cgaccgccgc gttgatgccg ctttgcatct gtagcatccg
     1921 ggtgaggcgc aggatcatct cctgctgatg ctttcgctcg ctgatgtcgc gttgagttcc
     1981 gcgatacccg cagacctgtc cgctctcgcc gatgagcgca gtgaggttgc attccagcca
     2041 gcgatgttgc ccgcttgcat gcctccagcg cgtgacgatc ccggaagcga tcttctgcgc
     2101 agcgctcagc cgcgccagcg tgccgccgaa gaccgcacga tcgtcttcgt gaatgagccg
     2161 ctcgaagcgc gcgcccaaca cgtcctggac cgatatcccc agcgcgccgc gcaccgactc
     2221 gctgctgaag acgaattcgc cgccggcatc catctcccag atccagtcct gcgaggtgtc
     2281 gacgatgtca cgcaggcgct gctcgctctg gcggatccgg ctctccgcca gcctgcgccc
     2341 ggcgcgctcg ctcacttcct tcagcgcgcg gcgcacggca ggaacgagcc gcccgggatt
     2401 gctcttcaga acgtagtcca ccgctccgcg gcgcagcgcc tcgatggcgc gctcttcgcc
     2461 gatcgtgccg gacaggaaga tgaagggcac ttcgggtacg gactccacgg caatctgcag
     2521 cgcggtcatc ccgtcgaagc ccggcaggcc gaagtcggac aggatcaggt gcgggcggaa
     2581 gcggctcagg gcagcgcgga agctcgcctc ggaatcggcc ctgctgtaga cacagcggat
     2641 accgccgcgg gcgagttgtt gaatcgcgag gtcggcttcg acgcgaacat cctcgagcac
     2701 cagcacgcgt aaaccgtgcg actccaagtt gcgcgacgct cctttctgca gccggcgcgg
     2761 cccggcatgg gccggatccg gcctctcgca gaattactgt cgcagatcgc gcgaatatct
     2821 cagacggagc gccggtcgcg ctcagtcctc gctgatgttg cggaagtcca cccccaggga
     2881 acgcagcttc cgatacaggt gggtgcgctc cataccgact cgcttggcga gctgtccgac
     2941 cttcccattg cacagcagaa gctgctgctg caggtaggcg cgctcgaact gctcgcgtgc
     3001 ctcgcgcaac ggcagagcga gcagatcctg cttgacgagc ggctcgcccg caggggcctg
     3061 tacggccagc tcccgctcga tttcctcgag acggatctcc tcggcgccgc cctggatcag
     3121 cagccgctgc accagattct tcagctcccg cacattgtcg ggccagggat agttgcgcag
     3181 ccgattctgc gcggccacgc tgaagcggcg gaactgcaag ccctcggtgt ccacgagacg
     3241 atcgacatag tggcgcagca actcgggaac gtcttccgcg taatcccgca acggcggcac
     3301 tctgacgatc aacgtgttca gatgcgccag caggtcccgg cgcaaacctt ccgcgccggc
     3361 tcgatgctcg acccccggct gcgccgagga gagaacccgg gcagtgagct gcaccggccc
     3421 ggttccaccg aggcgggtga actgtcccga ctcgagcaca cccaccagta aacgctgagc
     3481 agccggcggc aggtcctcga tctcgtgaat gaacagcgtg ccatcggcgg cttcctcgag
     3541 aaggccgatc tgcttacccg atgtctcccc ctcgcgcccg aagagccggg cctcggcatc
     3601 cgcctcgcgc agactgctgg cgatcagcgt cacgaacggg cggctccccc ggggtccgcg
     3661 ctcgtgcagg taacgtgcga acgcctcgcg tcccgatccg ggctcgccga tcaacagcac
     3721 tgtagaggga ttgagcgcaa tctgctgcag ctcaccccgc aactgctgca tctgcttgct
     3781 cttgccgacc ggcaccatca gcgccggaac gatgaacttg cccgattgcc ggcggcgctt
     3841 gcccgcgtcc agcgcccgct cgacggtgcg cagcagcttt gccagagaga gtggtttctc
     3901 gacgaaatcg aaggcaccga ggcgagtggc ctcgacggcc gtttctacag tgccgtggcc
     3961 tgacatcatg acgaccgggc agccgtcggt cgccgatacc gaccactcac gcagcagact
     4021 gatgccatcc acgtccggca tccagatgtc gagcagcacc agatccggcg tctgacgggc
     4081 acgggaggcg cgcgcttgtg ccgcattggc ggcgacatcc acctcgtagc cct
//
LOCUS       DASRHN010000240         8952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_78745, whole genome shotgun sequence.
ACCESSION   DASRHN010000240 DASRHN010000000
VERSION     DASRHN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78745"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFB37_09570"
     CDS             <1..361
                     /locus_tag="VFB37_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZO22736.1"
                     /translation="CYDVRFPELYRRSSLAGAQILSVPSAFTSPTGRAHWETLLRARA
                     VENLCYVLAPAQSGFHPNGRETYGDSMIVDYWGRILKRLPRGRGCVVAEIDLARQAEV
                     RQHFPALQHRVLQESNI"
     gene            361..1824
                     /gene="tldD"
                     /locus_tag="VFB37_09575"
     CDS             361..1824
                     /gene="tldD"
                     /locus_tag="VFB37_09575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HZO22737.1"
                     /translation="MDLPVADGAADALSLARDLILRPSGLDDNRLERVFGEVLSHSVD
                     FADLYFQLAHEEAWSLEDGIVKDGSASIEQGVGVRALAGEKTGFAYSDEIVLPALEEA
                     SRAARAIARQGGARRLQAWQARAGHRLYLPADPGASLSDPDKVAWLERVDRETRRLDP
                     RIVQVNASLTAVHEVVLIATSDGELAADVRPLVRFNVTVLVEQNGRREQGYAGAGGRI
                     ALADLVNSERPLALGREAVRQALINLEAVPAPAGPMTVVLGPGWPGILLHEAIGHGLE
                     GDFNRKGTSAFANRIGERVAAAECTIVDDGTLAQRRGSLNIDDEGTRTQCTTLIENGI
                     LRGYIQDRLNARLMGAQPTGNGRRESFAHMTLPRMTNTYMRPGPHDPAEIIRSVERGI
                     YAVNFGGGQVDITSGKFVFSASEAYAIENGKLQAPLKGATLIGNGPDVLTRVSMVGND
                     LKLDDGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            2250..2420
                     /locus_tag="VFB37_09580"
     CDS             2250..2420
                     /locus_tag="VFB37_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22738.1"
                     /translation="MHAEPALTDTQKLQLPPDLVSALAKRPRKPELDYGIESLKPSLF
                     SRLIEKLIGPKN"
     gene            2493..2744
                     /locus_tag="VFB37_09585"
     CDS             2493..2744
                     /locus_tag="VFB37_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22739.1"
                     /translation="MARQPLRLSRPIYEGLPWIYVACGLAALIGSYRSTSRPVSLLIG
                     VAGLLAVLAGVVLLLRRRDYRELRSQYGNPDSMREPGQD"
     gene            complement(2751..4046)
                     /locus_tag="VFB37_09590"
     CDS             complement(2751..4046)
                     /locus_tag="VFB37_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO22740.1"
                     /translation="MYLESFKLKELPFRLSPDPQFLYLSKQHARAKAYMESTIWFTDG
                     FVVITGEIGSGKTTLIESFLKEIQSDVVIAQINQTQVSAVDFLQAVLVQFGFSPFKMK
                     KAELIATLNNFLVEQYAAGRKVLLIVDEAQNLSMRVLEEIRLLSGVETTKEKVLRIIL
                     AGQPELNEKLDAPELVQLTQRIRLRFHLTTLSPPETRAYIQHRLEVAGAGDRPVFSEE
                     TYPEIYRFSGGVPRLVNTLCDTALMAAFTADRDTVTLADIRQSLEELQWVDYAARSAQ
                     QVRPAAASPPPPLELPPPTGAPATEAPQPVHGRLLVATDGRTVEERMLRVGRIIVGRT
                     PDNDLQIDSRFVSRHHCQIITTINSCVIEDLNSTNGIYVKSKRVRRHYLNDGDVVVIG
                     KHELIYVDERLARTRSSMTDTVPGVHPGMEPALDDEPME"
     gene            complement(4142..5257)
                     /locus_tag="VFB37_09595"
     CDS             complement(4142..5257)
                     /locus_tag="VFB37_09595"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZO22741.1"
                     /translation="MTVGIVGAGQLGRMLALAGYPLGLDFLFLDPAPQPPAGAVAPVL
                     RGAFTDTRLLGQLSRRCEVITFDWENVSVDALRALPRAARICPPIPALAASQDRMSEK
                     RLFERLEIPTTRWRAVSSRAQLSRALRDIGLPGVIKTRRMGYDGKGQAVVRTPEDTER
                     AWNELGNVPLLYEEYVPFDCEVSIIGARSGRGEFVVYPLNGNIHAQGMLRLTRAPFGP
                     PRWHTLAARYLQRVVTHFRYTGIATIEFFVRRGRLIANEMAPRVHNSGHWTIEGAETS
                     QFENHLRGILGLPLGSTRPRGFSAMINLIGSMPSRTAVLQQAGVHLHDYGKQARPGRK
                     LGHCTLVEASARARDARARRLLAQIGAPGWHPLRSGR"
     gene            complement(5254..5754)
                     /gene="purE"
                     /locus_tag="VFB37_09600"
     CDS             complement(5254..5754)
                     /gene="purE"
                     /locus_tag="VFB37_09600"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZO22742.1"
                     /translation="MKALVGIIMGSSSDWETMQAAADLLDRLSIPYEVRVVSAHRTPD
                     LLFEYASSARSRGLEVIIAGAGGAAHLPGMTASKTSLPVLGVPVPSKALAGLDSLLSI
                     VQMPAGVPVATFAIGTAGAANAALCAASILANRHAAIATALEGYRERQTAGVLDHPDP
                     RTTTKA"
     gene            complement(5786..6301)
                     /gene="yjgA"
                     /locus_tag="VFB37_09605"
     CDS             complement(5786..6301)
                     /gene="yjgA"
                     /locus_tag="VFB37_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HZO22743.1"
                     /translation="MTVHDDDPPVDEKPSRSARKRTAQAAQELGEQLIGLRDAELARI
                     GLPEELTEAIRVARRITSRGGGARQRQYIGKLMRQIELEPIRAALRAQGEQAAREAER
                     FKRLEAWRERLICGGATAVEELERWRPGLDRTHWSRLIGAAQSERAAGQGVAAARELF
                     RALRDLFGAEP"
     gene            6313..6441
                     /locus_tag="VFB37_09610"
     CDS             6313..6441
                     /locus_tag="VFB37_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22744.1"
                     /translation="MSRQSVTLALPGTRTEAALAGHTGEPLEGLYNRNDQPTDQRM"
     gene            6441..7790
                     /gene="pmbA"
                     /locus_tag="VFB37_09615"
     CDS             6441..7790
                     /gene="pmbA"
                     /locus_tag="VFB37_09615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZO22745.1"
                     /translation="MQQATAGETGIAQLQQVVRFVLEEAQQRGATQAEADASVNQGLG
                     VTVRLGEVETVEYQRDRGLGVTVYFGHRKGSASTADLGMTAVRETVEKACTIARFTAE
                     DTFAGLVDPEALARQIPELDLDHPWALAPEAAIALAQRCEAAGLAVGSQISNSEGASV
                     HTQRHLGVYGNSLGFLGGAVGTSHSMSCTLIAQQQETMERDYWYTTSRDPADLEAPEH
                     VGRKAGERAIARLGASKLSTRKAPVLFSPEMARGLFRHFIGAIRGSSQYRKASFLLNA
                     AGERIFPAFLQMQERPHIPKGLASAAFDAEGAATHDRDLVSDGVLQGYVLGSYSARKL
                     GLKTTGNAGGIHNLLVGSSAKASDLQALLRQMHTGLLVTEMMGQGVNGVTGDYSRGAS
                     GFWVENGAIVHPVHEITIAGNLRPMYQDIVAIGADVDHRGGIRTGSVLIGEMTIAGD"
     gene            complement(7828..>8952)
                     /locus_tag="VFB37_09620"
     CDS             complement(7828..>8952)
                     /locus_tag="VFB37_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZO22746.1"
                     /translation="LEPIVYEFFRPVGGQAAQELTEHVLQFAHRVSSGIVGSLGFALL
                     AWTLLGTIKKVEDSFNFLWRVEQPRSFARRIAEYLTLLIIGPVLLVAFVGLSHAAIAS
                     VPMQTMANLPLLQRLHGIALALAPYVMVTAFFTGMYMFIPNTRVRWRPALVGAAAGGV
                     LWAAVGKMFTAFVVYSTRLTIVYAGFAFIVTALLWTYSGWLILLAGAQLSFYVQNPTY
                     LRLGLQELQLSCVELEELALRTMYLVGRAHLTGGARWTVNGLASELGLPGIAVAKMAT
                     ALERAGLIVVTEENELLLARDLGRVLVTGILDLARNQRSGHAAPRTLPIPAVDRLLAG
                     LEEARRARCSDMTLRELVEDGTLRLAEAGPRPLQRGEHKA"
BASE COUNT         1557 a         2884 c         3022 g         1489 t
ORIGIN      
        1 ctgctacgac gtgagattcc cggagctgta tcgcaggtcg agcctcgccg gtgcgcagat
       61 cctgtcggtg ccctcggcgt tcaccagtcc aaccgggcgt gcgcactggg aaaccctgct
      121 gcgggcgcgc gcggtcgaga atctgtgcta cgtgctggcg ccggcgcaat ccggattcca
      181 cccgaacggg cgcgagacct acggtgacag catgatcgtg gactactggg gccggatcct
      241 gaagcgcctg ccccgcggtc gcgggtgcgt cgttgcggag atcgatctcg cgcgccaggc
      301 ggaggtgcgg cagcatttcc ccgcgttaca acatcgtgtt ctgcaggaga gcaatatctg
      361 atggacttac ccgttgcaga tggcgccgcc gacgccctga gcctcgcgcg tgacctgatc
      421 cttcggccga gcgggctcga tgacaatcgg ctcgagcgtg tgttcggcga ggtgctcagc
      481 cactcggtgg atttcgccga cctgtatttt cagcttgcgc atgaggaggc ctggtcgctc
      541 gaggacggca tcgtcaagga cggcagcgca agcatcgagc agggggtcgg ggtgcgtgcg
      601 ctggcgggcg agaagaccgg ctttgcttat tcggatgaga tcgtgctgcc ggcgctggag
      661 gaagcctccc gcgcggcgcg agccatcgcc cggcagggcg gcgcccggcg gctgcaggcc
      721 tggcaggccc gcgcagggca tcggttgtat ctaccggccg acccgggcgc ctcgctctcc
      781 gatccggaca aggtagcgtg gctggagcgt gtcgaccggg agacgcgccg cctggatccg
      841 cgcatcgtgc aggtgaatgc cagcctgacc gcggtgcacg aggtcgtgct gatcgcgacc
      901 agcgacggcg agctggccgc cgacgtgcgg ccgctggtgc gattcaacgt caccgtgctc
      961 gtcgagcaga acggccggcg cgagcaaggc tacgctggcg ccggcggacg catcgcgctg
     1021 gcggatctgg tgaactcgga gcgtcccctc gcgctcggcc gcgaggccgt tcgccaggca
     1081 ctcatcaacc tggaagccgt gccggcgccg gccggaccga tgaccgtcgt gctcgggccc
     1141 gggtggcccg gcatcctgtt gcacgaggcg atcggacacg ggctcgaggg cgatttcaac
     1201 cgcaaaggca cctcggcctt cgccaatcgg atcggtgagc gagtggccgc cgcggaatgc
     1261 acgatcgtcg atgacggtac gctcgcgcag cgacgcggat cgctcaacat cgatgacgag
     1321 ggcacgcgaa ctcagtgcac cacgctcatc gagaacggga tcctgcgcgg ttacattcag
     1381 gaccggctga atgcgcgcct gatgggcgca cagcccacgg gcaatggccg gcgtgagtcc
     1441 ttcgcgcaca tgaccttgcc acgcatgacc aacacgtaca tgcgacccgg tccgcacgat
     1501 cctgcggaga tcatccgctc ggtcgagcgt ggcatctatg ccgtcaactt cggcggcggt
     1561 caggtggata tcacttccgg gaagttcgtg ttctcggcca gtgaggcgta cgcaatcgag
     1621 aacggcaagc tgcaggcgcc gttgaagggc gcaacgctca tcggcaacgg gcctgatgtc
     1681 ctcacgcgcg tctcgatggt cggtaacgat ctgaagctcg atgatggtgt gggcacctgc
     1741 ggtaaggaag gacagagcgt tcccgtcggc gtcggccagc cgacacttcg catcgatggt
     1801 cttacagtgg gcggaacggg ctgaaagagc gcattttcat gtaaatgcgc ggctcggtgc
     1861 gcttgtgtcc gaattcgggc gcatgccggc cacgaatgcc ctctcgtccc tcttgcggtg
     1921 ttggtgcagt ctgacacgcg cggtcggagc aggagttccg cttgcgcagg gatgcgcaga
     1981 ccgtgcgccg gacagagcgc gggccactcg caaatctgcg cgccccgtct gcaatgcgtg
     2041 ctcgtcctca gcgtctgcgt tcgcagactt gcgacacgtg gcggaattgc gtgatgcgga
     2101 cataattgca tccgcatgac ggctgtgtcc gctttccaca aagagccaat gcggaacgat
     2161 gacgaggtgt gacgggctgc acgccgccgc gctccatggg tccgtagctt gctgggtcag
     2221 attcgctcac ttcagtcgag gattggacga tgcacgcaga gcccgcactc accgacacgc
     2281 agaagctgca acttcctccg gatctcgtga gcgcgctggc caagcgcccc cgcaaaccgg
     2341 aactggacta cggcatcgaa tcgctgaagc cctcgctgtt cagccgcctc atcgagaagc
     2401 tcatcggacc gaagaactga gccggggcga ggcgtccgcc tgcgccgacg ccgacgccat
     2461 gcgcgatttc ccgccgtgct ttaggattag gcatggcccg acaacctctg cgcctgtccc
     2521 gccccatcta cgaggggctg ccctggattt acgtcgcctg cgggctggca gcgctcattg
     2581 gcagctatcg aagcacatcg aggcccgtga gcctcctcat tggcgttgcg ggactgctcg
     2641 cagtcctggc gggggtcgtg ctgctgctcc gccggcgcga ttaccgggag ctgcgctcgc
     2701 aatacggcaa cccggactcg atgagagagc ccgggcagga ctgatcctcg ctactccatg
     2761 ggctcgtcat cgagggccgg ctccatcccc gggtgaacgc ccggtacggt gtcggtcatg
     2821 gagctgcgcg tgcgcgccag gcgctcgtcc acgtagatca gctcgtgttt gccgatgacc
     2881 acgacgtcac catcgttgag gtaatggcgg cggacgcgct tggacttcac gtagatgccg
     2941 ttcgtgctgt tgagatcttc gatcacgcac gagttgatgg tggtaatgat ctggcaatga
     3001 tggcggctga cgaatcggct gtcgatctgc aggtcgttgt caggtgtgcg gccgacgatg
     3061 atgcgtccaa cacgcagcat ccgctcctcc acggtgcgtc catcggtagc gacgagcagc
     3121 cgcccatgta ccggctgtgg ggcctccgtc gcaggcgctc ccgtcggggg cggcaattcc
     3181 agtggcggtg gcggcgaagc tgccgccggc cgcacctgct gcgcgctgcg cgcggcgtag
     3241 tccacccact gcagctcctc gagggactga cggatgtcgg cgagtgtcac cgtgtcgcgg
     3301 tcggcagtga aggccgccat gagcgcggta tcgcacaagg tattcaccag tcgcggcacg
     3361 ccacccgaga agcggtagat ctccggatag gtctcttcgg agaaaaccgg gcggtcgccg
     3421 gcaccggcta cttccagccg gtgctgaatg taggcacgcg tctccggcgg ggacagcgtg
     3481 gtgaggtgaa agcgcaggcg aatgcgctgg gtaagctgca cgagctcggg cgcatcgagc
     3541 ttctcgttga gctccggctg gccggccagg atgatgcgca gcaccttttc cttggtggtc
     3601 tcgactccgg aaagcaggcg gatctcctcc agcacgcgca tggagaggtt ctgcgcctcg
     3661 tcaacgatga gcaacacctt gcgcccggcc gcgtactgct cgacgagaaa attgttcagc
     3721 gtcgcgatca gctccgcctt cttcatcttg aagggcgaga agccgaactg aacgagcacg
     3781 gcctgcagga aatcgaccgc cgagacctgc gtctggttga tctgcgcgat caccacatcc
     3841 gactggatct ccttcagaaa cgactcgatg agcgtcgtct tgccggagcc gatctcgccc
     3901 gtaatcacca cgaaaccgtc ggtgaaccag atcgtggact ccatgtacgc cttggcgcgg
     3961 gcgtgctgct tggacaggta caggaactga ggatccgggc tcagtcgaaa gggcagctcc
     4021 ttcagtttga acgattcgag atacatgcgt gctcgtgtgg gaaacggctg cctggcggat
     4081 tgttagctgt ttgcgaagcg tcacggcgga cgatcggcga gcagctgttc gctgggaaaa
     4141 gttacctgcc cgatcttaag ggatgccaac ccggggcgcc aatttgggcg agcaaccggc
     4201 gtgcccgtgc atcgcgggcc cgggcgctcg cctccaccag ggtgcaatgt ccgagcttgc
     4261 ggcccgggcg cgcctgtttg ccgtagtcgt gcagatggac ccccgcctgc tgcagaaccg
     4321 ccgtccggga gggcatgctg ccgatgaggt tgatcatggc gctgaagccc cggggccgcg
     4381 tggagcccag cggcagcccg agaatgccgc gcaggtggtt ctcgaactga ctggtctctg
     4441 cgccctcgat ggtccagtgg ccggagttgt gcacgcgggg tgccatctcg ttggcgatga
     4501 gtctcccacg acgcacgaag aactcgatgg tggcgattcc ggtgtagcgg aaatgggtga
     4561 ctacccgctg caggtagcgg gctgcaagtg tgtgccagcg cggcgggcca aaaggcgcgc
     4621 gcgtgaggcg cagcatccct tgcgcatgaa tatttccgtt cagggggtag acgacaaact
     4681 cgccgcgacc gctgcgagcc ccgatgatcg agacctcaca gtcaaacggc acgtactcct
     4741 catagagcaa gggcacgttt cccagctcat tccacgcacg ctccgtatcc tcgggtgtgc
     4801 gtactaccgc ctggcccttg ccgtcatagc ccatgcgacg cgtcttgatg acgccgggca
     4861 gcccgatgtc acgtaacgcg cgtgaaagtt gcgcgcgcga gctgacagca cgccagcgtg
     4921 tagttgggat ttcgagccgc tcgaacaaac gtttctcgct catgcggtcc tgggaggcgg
     4981 cgagcgccgg tatgggcggg cagatgcgcg ctgcccgcgg caaggcacgc aacgcatcga
     5041 ccgacacgtt ttcccaatcg aaggtgatga cctcgcagcg gcgggaaagc tgcccgagca
     5101 gcctcgtatc ggtgaacgcg ccccgcagca ccggcgccac cgcgccagcg ggaggctgcg
     5161 gcgccggatc caggaacaga aagtccagac ccagtggata gcccgccagg gcgagcatgc
     5221 ggccgagctg gccagcgccc acgataccga cggtcatgcc ttggtcgtcg ttcgcgggtc
     5281 aggatggtcg agcacgccgg cggtctgacg ctcgcgatag ccttcgagcg ccgttgcgat
     5341 cgccgcatgc ctgttggcca gaatcgaggc cgcacacagg gcggcattgg ctgcccctgc
     5401 agtgccgatg gcgaaagtgg cgaccggaac gccggccggc atctgcacga tcgacagcag
     5461 ggaatcgagg ccggccagcg ccttcgaggg caccggcact ccgagcaccg gcaggctggt
     5521 cttggaggcg gtcatgccgg gcagatgcgc cgctcccccc gccccggcga tgatgacctc
     5581 caggccgcgc gagcgcgcgc tggaggcgta ctcgaagagc agatcggggg tgcgatgggc
     5641 tgaaaccacc cttacttcgt aggggatcga cagtctatcg agcagatcgg ccgccgcctg
     5701 catcgtctcc caatcggagc tcgagcccat gatgatgccg acgagcgctt tcatcgggac
     5761 attgtaccgg gggccggttg ccctttcatg gctcggcgcc aaacaggtcc cgcagggcgc
     5821 ggaacagttc ccgggcggcc gcgacgccct ggccggcagc ccgctcgctc tgtgcggcgc
     5881 cgatcaagcg cgaccagtgt gtgcggtcca gacccggccg ccagcgctcg agctcctcca
     5941 cagccgtcgc tccgccgcag atcagccgct cgcgccaggc ctccagccgc ttgaagcgtt
     6001 ccgcctcgcg ggccgcctgc tcaccctgcg ctctcagcgc cgcgcggata ggctcgagct
     6061 cgatctgccg catcagcttg ccgatgtatt gtcgctgacg tgccccgcca cctctgctcg
     6121 tgatccggcg tgccacgcgg atggcctcgg tcagctcttc gggcaggccg atgcgggcga
     6181 gctcagcatc gcgcaggccg atgagctgct ctccaagctc ctgcgcggcc tgcgccgtgc
     6241 gcttgcgggc gcttctgctg ggcttctcgt caactggcgg gtcgtcgtcg tggaccgtca
     6301 ctgaggtatt ccatgagtcg gcagagtgtg actttagctc tgccgggcac ccgaaccgaa
     6361 gcggcgctcg cggggcatac cggcgagccg ctcgaaggcc tgtacaatcg caacgatcag
     6421 ccaacagacc agcggatgtg atgcaacagg caacggccgg ggaaacggga atcgcgcagc
     6481 tccagcaggt cgtgcgtttc gtgctggagg aagcgcaaca acgcggtgcg acgcaggccg
     6541 aggccgacgc gagcgtcaat cagggactgg gcgtcaccgt gcggctgggc gaggtcgaga
     6601 ccgtcgagta ccagcgcgac cggggtctgg gtgtcacggt gtacttcgga caccgcaaag
     6661 gctcggccag cacggcggac ctgggaatga cggccgtgcg cgaaacggtc gaaaaggctt
     6721 gcacgatcgc ccgcttcaca gctgaagata cctttgcagg gctggtggat ccggaagcgc
     6781 tcgcgcgcca gatcccggag ctcgatctgg atcatccctg ggcacttgcg ccggaggccg
     6841 ccatagcgct tgcccagcgc tgcgaagcag cgggtctggc ggtgggctcc cagatctcga
     6901 attccgaggg cgcatcggtg cacacacagc gccatctggg agtgtatggc aactcgctgg
     6961 gctttctcgg cggggccgtg ggcaccagcc actcgatgag ctgcacgctc atcgctcagc
     7021 agcaagagac gatggagcgc gactactggt acacgacctc gcgcgatccg gcggatctcg
     7081 aggcgcccga gcacgtaggc cgcaaggcgg gcgagcgagc catcgcgcgg ctcggggcga
     7141 gcaaactctc cacgcgcaag gcgccagtcc tcttctcacc cgagatggcg cgcggcctgt
     7201 tccggcactt catcggcgcc atccggggct cgagccagta ccgcaaggcc tcctttctgc
     7261 tcaatgccgc cggtgagcgg atctttcccg cgttcctgca gatgcaggag cggccgcaca
     7321 ttcccaaggg actcgcgagc gccgcattcg atgcggaggg cgctgccacc cacgatcgcg
     7381 atctggtgag cgacggcgtt ctgcagggct acgtgttggg aagctactct gcgcgcaagc
     7441 tcgggctgaa aaccaccggt aacgcaggcg gcatccacaa cctgctcgtg ggctcatccg
     7501 caaaggccag cgacctgcag gcattgctgc gacagatgca cacgggactg ctggtcacgg
     7561 aaatgatggg ccagggcgtc aacggggtca cgggcgacta ctcacgcggt gcgagcgggt
     7621 tctgggtcga gaacggggcg attgtccatc cggttcacga gatcacgatc gccggcaacc
     7681 tgcgccccat gtatcaggac atcgtagcga tcggcgcgga tgtggatcat cgcggtggga
     7741 ttcggacggg ctcggttctc atcggcgaga tgacgatcgc cggcgactga gctcggggca
     7801 actcctcgct cgcccacgcg tgcgcagtca ggccttgtgc tcaccgcgct ggagcggccg
     7861 cggtccggcc tcagccagac gcagggtgcc gtcttccacc agctcccgca gggtcatatc
     7921 actgcagcgt gcgcgccgag cctcctccag gcccgccaga agccggtcta ccgcgggaat
     7981 cggcaaagtg cgcggcgcgg catgcccgct gcgctgattg cgggccagat cgagtatgcc
     8041 ggtgacgagc acgcgcccga ggtcgcgggc gagcagcagc tcattctcct cggtgaccac
     8101 gatgagtccg gcgcgctcga gcgcggtggc catttttgcc acggcgatgc cgggcagacc
     8161 cagctcactg gcgagaccgt tcacggtcca gcgggctccg ccggtcagat gggcgcggcc
     8221 cacgaggtac atcgtgcgca gtgcgagctc ttccagctcg acacacgaga gctgcaactc
     8281 ctgcagaccg agccgcagat acgtggggtt ctggacatag aacgaaagct gcgcgccggc
     8341 gagcaggatc agccagccgg aataggtcca cagcaacgcc gtcacgatga atgcaaaccc
     8401 ggcgtacacg atcgtgaggc gcgtcgagta gacgacgaac gcggtgaaca tcttgccgac
     8461 tgccgcccat aacacgccgc ccgcggcagc ccccaccaga gcagggcgcc agcgtacccg
     8521 cgtgttggga atgaacatgt acatgccggt gaagaaggcc gtgaccatca cgtaaggggc
     8581 gagcgccagc gcaattccgt gcagccgctg aaggagcggc aggttggcca tcgtctgcat
     8641 cggcacgctc gcaatggccg cgtgcgagag ccccacgaat gccaccagca gtaccggacc
     8701 gatgatgagc aatgtcaggt actcggcaat gcggcgcgcg aagctgcgcg gctgctccac
     8761 gcgccacagg aaattgaagc tgtcctccac cttcttgatc gtcccgagca gcgtccaggc
     8821 gagcagggcg aagcccaacg atcccacgat gccgctgctg acacgatgcg cgaactgcag
     8881 aacgtgctcg gtgagctcct gagccgcctg gccgccgacg gggcggaaga actcgtacac
     8941 gatcggctcg ag
//
LOCUS       DASRHN010000241         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2058998, whole genome shotgun sequence.
ACCESSION   DASRHN010000241 DASRHN010000000
VERSION     DASRHN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058998"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /gene="cmk"
                     /locus_tag="VFB37_09625"
     CDS             complement(<1..484)
                     /gene="cmk"
                     /locus_tag="VFB37_09625"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZO22747.1"
                     /translation="MAAPPIVTIDGPSGSGKGTISRAVAARAGWHLLDSGALYRLVAV
                     AGLQAGLDPADLAGHARLAATLNASFSVEPGGAERVLLDGVEVTSRIRSEEAGQGASR
                     VASWPEVRRALLERQRAFARPPGLVADGRDMGTVVFPTATLKVFLTASLEERALRRYK
                     Q"
     gene            complement(525..1829)
                     /gene="aroA"
                     /locus_tag="VFB37_09630"
     CDS             complement(525..1829)
                     /gene="aroA"
                     /locus_tag="VFB37_09630"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZO22748.1"
                     /translation="MSAYRALPGGVLAGELTVPGDKSISHRALMLGGIAHGDTEITGF
                     LAGEDCLATLRALRQLGVRIEQPQDQHIIVHGAGSEGLRAAAAPLDLGNAGTAMRLFM
                     GLLSGRPFDSTLVGDESLMRRPMERVAKPLRQMGADVETQSGLPPVVIRGGRPLRSID
                     YVLPVASAQVKSALLLAGLQAVGITRITEPAPTRDHTERMLRAFAVPVQSSGSTVVLE
                     GRCALQGTRVVVPADFSSAAFFLVGGCLAAERELVLRNVGVNPTRTGLLQLLLQMGGD
                     IRVHPHEETGEGEPIADIVVRKSELRGISVGGDLAALSIDEFPVFFIAAACAQGATVV
                     RDAAELRVKETDRLALMAQGLTQLGVQNRLQPDGLWIAGGGGFCGGSIDSGGDHRIAM
                     AFSIAALRARAPIEIRDVANVATSFPGFDALARKAGLQIESH"
     gene            complement(1832..2905)
                     /gene="pheA"
                     /locus_tag="VFB37_09635"
     CDS             complement(1832..2905)
                     /gene="pheA"
                     /locus_tag="VFB37_09635"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017816350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZO22749.1"
                     /translation="MEQVRREIDAIDERIHTLINDRARLAQQVGISKSRDGRTVDFYR
                     PEREAQVLRKARARNAGPLRDEEVVRLFREIMSACLAQQEPLKVAFLGPEGTFTQSAV
                     LHHFGHSVRALSVASIDEVFHEVEAASADFGVVPIENSTEGTVNHTLDRFLSSPLKIC
                     GEVELRIHHCLMGLMGSLGRIARICSHSQSLAQCRVWLDEHLPEVERVPVASNAEGAR
                     RARDEKGTAAIAGDTAAEVYGLKVLVSDIEDRPDNTTRFFVLGRKLFAPSGADRTTLL
                     VSVSHTDAPGALHRLLEPLARHNVSMTRIESRPSHRRKWDYVFFIDVEGHADEPHVAR
                     ALAELKRRSSLLRILGSYPQAVL"
     gene            complement(2980..>3602)
                     /locus_tag="VFB37_09640"
     CDS             complement(2980..>3602)
                     /locus_tag="VFB37_09640"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZO22750.1"
                     /translation="EQALSLEDLCARADVISVHVPLNSHTQHLLNETRLKLMRKSGII
                     LNFARAAIVDDQAVVAALDAGRLHAYVCDFPSNLLKDHPQVVTLPHLGASTGEAEENC
                     AVMVAEQVRDFLENGNIRNSVNYPESIMPRAPNTTRIVIANNNVPNMVGQISTCLAGA
                     RINIADLLNKSRGEYAYTLIDTDGEVGEEILGSIRAIEGVLSARIL"
BASE COUNT          625 a         1230 c         1173 g          574 t
ORIGIN      
        1 actgcttata acggcgcaga gcccgctcct cgaggctggc cgtgagaaat actttgagcg
       61 tggccgtggg gaataccacg gtccccatgt cccggccgtc ggccaccagc cccgggggcc
      121 gggcgaaggc ccgctgccgc tccagcaggg cccgccggac ctctggccag gaggctacgc
      181 gggaggcccc ctgccccgcc tcctcggagc ggattcgtga ggtcacctcg actccatcga
      241 gcaggacgcg ctcggcaccc ccgggctcga ccgaaaagct cgcattcagg gtagcggcca
      301 gccgggcgtg accggccagg tcagccgggt cgaggccggc ctgcagaccc gccaccgcga
      361 ccaggcggta cagagcgccg ctgtcgagca ggtgccaccc ggcgcgtgcg gcgactgccc
      421 ggctgatggt gcccttaccc gagccgctcg ggccgtcgat ggtgacgatg gggggcgctg
      481 ccaaggggag attcctgctc agaaggctgc cacgctaagg tccgtcagtg tgactcgatc
      541 tgcaagcccg ccttgcgcgc cagcgcatcg aagcccggaa acgaggtcgc gacattggcc
      601 acgtcccgga tctcgatggg ggcccgggca cgcagcgcag cgatgctgaa agccatggcg
      661 atgcggtggt ccccgccact gtcgatgctc ccgccgcaga agccgccgcc gcctgcgatc
      721 cagagtccat cgggctgcag acgattctgc acccccagct gggtgagccc ctgcgccatg
      781 agcgccaggc ggtcggtttc cttcacccgc agctccgcgg catcccgcac gacggtcgcg
      841 ccctgggcgc aggccgcggc gatgaagaac acgggaaatt cgtcgatcga gagcgcggcc
      901 agatcgccac cgacgctaat cccacgcagc tcgcttttgc gcaccacgat atcggcgatc
      961 ggctcgcctt cgcccgtctc ttcgtgcggg tggacgcgga tatcaccgcc catctgtaga
     1021 agcagctgca gaaggcccgt acgcgtcgga ttcacaccca cgttgcgtaa caccaactcc
     1081 cgctcggcag cgaggcagcc gccgacgagg aagaaagcag cagaggagaa gtcggccggc
     1141 acgaccacgc gcgtcccctg gagcgcgcag cggccctcga ggaccacggt cgaacctgag
     1201 ctctgtacgg gcactgcgaa tgcccgcagc atccgttcag tgtgatcccg tgtcggggca
     1261 ggctcagtga tgcgcgtgat ccccacggcc tgcagtcccg caagcagcag ggccgatttc
     1321 acctgggcgc tcgcgacggg cagcacgtag tcgatcgatc tgaggggccg gccgccgcgg
     1381 atcaccaccg gcggcaggcc actctgagtc tcgacatccg cacccatctg ccgcaacggc
     1441 ttcgccacgc gttccatcgg ccggcgcatc agggattcat cgccgaccag ggtcgaatca
     1501 aagggccgcc cagacagcag acccatgaac aggcgcatgg cggtgccggc attgcccagg
     1561 tccagtggcg cggcagctgc tcgcagcccc tcggatcccg ccccatgcac gatgatgtgt
     1621 tgatcttgcg gctgctcgat gcggacacca agctgacgca gcgcacgcag ggtggccagg
     1681 caatcctccc ctgcgaggaa gccggtgatc tcggtgtccc cgtgcgcgat cccgccgagc
     1741 atcagggccc gatgcgagat cgacttgtct ccggggacgg tgagctcgcc tgcgagcact
     1801 ccgccgggca gagcgcggta ggcactcatc ctcaaagcac cgcctgggga taggagccca
     1861 ggatgcgcag cagcgaggat cggcgcttga gctccgccag agcgcgtgcc acgtgcggct
     1921 cgtccgcatg cccctccacg tcgatgaaga agacataatc ccacttgcga cggtgcgagg
     1981 ggcgcgactc gatgcgcgtc atgctgacat tgtggcgggc cagtggctcg agcaaacgat
     2041 gcagggcgcc tggagcatcg gtgtgactga ccgagacgag cagcgtggtg cgatcggcgc
     2101 cgctcggggc gaacagcttg cgccccagaa cgaagaaacg cgtcgtgttg tcgggtcggt
     2161 cctcgatgtc ggagacgagc accttcagcc catacacctc cgcggcggtg tccccggcaa
     2221 tcgccgccgt acccttctcg tcacgtgcgc gccgagcgcc ctcggcgttg ctcgccacgg
     2281 gaacccgttc gacttccggc agatgctcat cgagccagac ccggcattgc gccagcgatt
     2341 gcgaatgcga gcagatgcgc gcgatgcggc cgagagagcc catcagaccc atgagacagt
     2401 ggtggatgcg cagctcgacc tcgccgcaga tcttcagcgg tgagctcaga aagcgatcga
     2461 gtgtgtgatt gaccgtgcct tcggtcgagt tctcgatcgg cacgaccccg aagtctgcgc
     2521 tggctgcctc gacttcgtgg aagacctcgt cgatggaagc caccgacaag gcacgcaccg
     2581 aatgcccgaa gtggtgcaac acggcgcttt gcgtgaaggt gccctcgggc ccgaggaaag
     2641 ccactttcag cggctcctgc tgcgcaagac aggcagacat gatctcgcga aacagccgca
     2701 cgacttcctc atcgcgcaac gggcccgcgt tgcgggcgcg cgccttgcgc aacacctgcg
     2761 cttcgcgctc cgggcgatag aaatcgacgg tccgaccgtc ccggctcttg gagattccca
     2821 cctgctgggc gagccgggcg cgatcgttga tgagcgtatg gatccgctcg tcgatcgcat
     2881 cgatctcgcg gcgtacctgc tccagatcgc tcgccggacc ccggggctga gcgggtgcgc
     2941 gaggggatcg gcccgcccct gcgggcttgc ggcgcgactt cacaggattc gtgccgacag
     3001 cacgccctcg atcgcgcgga tcgaccccag aatctcctct cccacctccc cgtccgtatc
     3061 gatcagcgta taggcgtact cgccgcgcga cttgttgagc agatccgcaa tattgatgcg
     3121 cgcgcccgcc aggcaggtcg agatctggcc caccatgttg ggcacgttat tgttggctat
     3181 cacgatgcgc gtggtgttgg gcgcgcgagg catgatgctc tcaggataat tgacgctgtt
     3241 gcggatgttg ccattctcga gaaagtcgcg gacctgctcc gcgaccatca cggcgcagtt
     3301 ctcttccgcc tcaccggtag aggcgccgag gtgcggcagt gtgaccacct gcggatgatc
     3361 cttcagcaga ttgctcggga aatcgcacac gtaggcgtgc agccgaccgg catcgagcgc
     3421 cgccaccacc gcctgatcgt ccacgattgc tgcacgggcg aaattcagga tgatcccgct
     3481 cttgcgcatg agcttcaggc gagtctcatt cagcaggtgc tgcgtgtggc tattgagcgg
     3541 cacgtgtacg ctgatgacat ccgcgcgcgc acacaggtcc tcgagtgaga gcgcctgctc
     3601 ca
//
LOCUS       DASRHN010000242        18335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_410680, whole genome shotgun sequence.
ACCESSION   DASRHN010000242 DASRHN010000000
VERSION     DASRHN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18335
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410680"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFB37_09645"
     CDS             complement(<1..389)
                     /locus_tag="VFB37_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZO22751.1"
                     /translation="MSEKARGYAHRIDIAADVSQVWRALTEDALLGRWCAPGAQIRAK
                     PGGSFRASVDRVTELEAHIDVFEPARRLRLIHLPSGSLPSADSAIVDDFILEPVEPGT
                     ILRLLGSGVPPTPEWDTQYRRMRMGWQ"
     gene            473..862
                     /locus_tag="VFB37_09650"
     CDS             473..862
                     /locus_tag="VFB37_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HZO22752.1"
                     /translation="MPWVHLVIGLALVEFLFFGIAVGRARARYGVHAPATTGHEVFER
                     YFRVHMNTLEQLVVFVPSILLFARYANPALAAALGAIFLVGRLLYFNRYVADPRKREL
                     GFVLSMLPTVILLVGGIIGAIRALLSG"
     gene            complement(864..1508)
                     /locus_tag="VFB37_09655"
     CDS             complement(864..1508)
                     /locus_tag="VFB37_09655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZO22753.1"
                     /translation="MLGWFIDLVLHLDRHLVELLARYDLWIYAILFAVIFCETGLVVT
                     PFLPGDSLLFAVGALAAVDPTGKLSAPLAFLLLAAAAVLGNIVNYSIGRAIGPPAFSG
                     RYPLLKVEYLRRTEEFFARHGAMAVALSRFMPIIRTFAPFVAGVGRMPYPRFLGYNLL
                     GGVSWVMLFLWGGYLFGNIPLVKQNFGVVTILIILASLVPLLVAWLRSRAAPTP"
     gene            complement(1515..2804)
                     /locus_tag="VFB37_09660"
     CDS             complement(1515..2804)
                     /locus_tag="VFB37_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZO22754.1"
                     /translation="MISTRSRTLIALCAGAVFGFSAATTSGVLAERPGQSAKADSVPA
                     ALPWEEARQFAEVFERIKREYVDDVDDHALMDKAIRGMVAALDPHSAYLDSEEFEEIR
                     LSTMGSYPGVGIEVVAEDSAVKILRPIEGSPAALAGLLPGDQITRIDDMDIGADLAGA
                     IARMRGSSGSLVKLTIRRPSTGQTLNFSLRRAKVEVHSVAEQDLEPGYGYVRISGFSE
                     TTPDDLGHAMERLKRDNPRGLKGLVLDLRNNPGGVLEAGVAVADAFLDEGVIVTADGR
                     TADARFSMEATPGDVLDGAPLVVLVNSGSASASEIVAGALKDHGRAELIGHKTYGKGS
                     VQTVMPLSHGGAVKLTTSRYFTPSGASIHGKGIVPDIVSEGPEEPPAELLSAKSAPSL
                     VSRDREVRLALHELKVQGAVARTPPTGIALMRAAFVP"
     gene            complement(2920..5175)
                     /locus_tag="VFB37_09665"
     CDS             complement(2920..5175)
                     /locus_tag="VFB37_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO22755.1"
                     /translation="MTDLRQTVSAPDPWGSFEPYVQLVRSLLPRATSVALYDALGDLR
                     WSSETTTGPDLHQLVESALAAGGTSPQGPGLMRTFDGNQPVYLCWIRDDSELLIGVVV
                     VLCRHCSDPEAEGRGFSFTHSLLRPALECLRRDLISRAAIDQLHRTVSSRDKDLELLL
                     ADGAPDRPTGDGADELKGILQQAIEHVGAVTTALIVPDKSIALLRSSAQRPADTQLVA
                     RAHRQLLSMAQIRREPVIINKIAPNSGMGVLPYKILSCALRSVAGKAVGVLALFREEA
                     GEAFSERDARLTEILARKAVGIIESSFDALSGLYTRPAFEQRIRAVVAGGSASRQWTA
                     LYIDADQLHVINDSFGMHVGDSVLGQLGELIRRRLPPGAFAARISGDRFAVLLPTDLD
                     HAEEFAESLREGVEQLGTSHGDSRLHVSISIGVALLDTGSGELMHSLAAAETACKAAK
                     DRGRNRVEAYKSNDVSLVRRFADINIAGELRAAIDAGRLRLDAQLILPFASADSPRPH
                     FELLLRMVDEEGRTVGPDRFLSAANRYQLMPEIDRWVITRAIELLKPQAGLLAGRSVS
                     FAINFSGQSLNDEDFVDFLLEAIRTSGLDPALFCFELTENATIANIARAESLIRRLRR
                     LGCGVALDDFGTGLSSLAYLRQLPVTMLKIDGSFVRDILKDPRAESMVRAIAQLARSM
                     SIATVAEYVETDEIRTRLATLGVDYGQGFAIGRPGPLIDLLAELPLLIATEPCASSDL
                     TTINRVVEIVH"
     gene            complement(5376..6587)
                     /locus_tag="VFB37_09670"
     CDS             complement(5376..6587)
                     /locus_tag="VFB37_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO22756.1"
                     /translation="MLTLTRRRFAVPHQRAGRLAVGLIALVACLPGHGAAPAPAKEPE
                     ADARRTEAQLQSVKAEIERITREVRQDQVERDRLTRELRSAEESVGKAREGLDSVRRN
                     RAVTAARRTSLAADRRTREADLAQNRGALAAQLRAAYLIGPEEPLKLLLNQQDPERAG
                     RMFVYYSYFGRARAQQIALIENDVHAISELDGQLESEDDRLAQLEAQQRSQLADLETA
                     RSRRSDVLASLTAESNTRAQSLERLKSQQSGLEKLLAELRRAIEQFPVNGNDAFARLR
                     GKLAWPVSGKLVARFGEQRAGGVKWDGVLVATDRGAPVRAIYRGRVIYADWLPGLGLL
                     SIVDHGDGYMSLYGHNERLYKAVGERVNAGDTLGSAGDSGGSSRPELYFEIRKAGKAV
                     DPRPWFKAAQP"
     gene            6652..7074
                     /locus_tag="VFB37_09675"
     CDS             6652..7074
                     /locus_tag="VFB37_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZO22757.1"
                     /translation="MDRLVEYVGHHPWYVAGALAAAILVMIYELRKRKESFAAVSPQD
                     LVRLMNQGALVIDLRPAEAFASGHLAGARHMSGEQVLKAGDTLKKHKEKTVVVYDDTG
                     SLSASAVRQLASQGFTRAFNLRGGLAAWRAENLPLSKA"
     gene            7084..7362
                     /gene="grxC"
                     /locus_tag="VFB37_09680"
     CDS             7084..7362
                     /gene="grxC"
                     /locus_tag="VFB37_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HZO22758.1"
                     /translation="MGPPEVLIYVTSWCPYCDRARRLLQSKGVPLREINIEEVPQSRA
                     EMVARSGRHTVPQIFVGEIHVGGSDDLYALEAEGRLDTLLKSGARNGE"
     gene            7352..7834
                     /gene="secB"
                     /locus_tag="VFB37_09685"
     CDS             7352..7834
                     /gene="secB"
                     /locus_tag="VFB37_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZO22759.1"
                     /translation="MANEMTGGTPMSDAQGDLGPMLALQNVYLKDCSYEAPNGPRVDG
                     NWNPQINLDLHTGASGLTPELREVVLTVTVSAKQNDATVFLVEVKQAGVFLMRNLSEV
                     DLKRAVSSVCPSVLFPYARAAISNLVTQGGFPQFLLPPVNFDALYAQSANQDQPVTTN
                     "
     gene            7869..8915
                     /locus_tag="VFB37_09690"
     CDS             7869..8915
                     /locus_tag="VFB37_09690"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZO22760.1"
                     /translation="MSAERPDSAPKGRADLHTPVAVLGAGSWGTALAIQFARSGRPTR
                     LWGRDRKQLLAMSSGRCNARYLASAQFPAALSVEPDLAAALSGTREALIAVPSHAFRG
                     LLLQVAPHLAADSALAWATKGFELDTGQLPHQVAREVLGLKRAIAVLSGPTFAREVGS
                     GLPTAMTIASPDSAFATALARELSSTNFRAYTSTDIVGVEVGGAVKNVLAVGAGLSDG
                     LGFGANTRVALITRGLVEMMRLGVAAGARRETFMGLAGLGDLVLTCTDDQSRNRRFGL
                     LLAAGRTAPEALAAIGQVVEGYTAAEATLRVARRMGVEMPLCEGVHRVLYESVPAREA
                     VRDLMSRPIRSEID"
     gene            8972..9958
                     /locus_tag="VFB37_09695"
     CDS             8972..9958
                     /locus_tag="VFB37_09695"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZO22761.1"
                     /translation="MSTMSSAAPVADDGLRLQFCGLEFASPIVLLSGCVGFGEEYTRI
                     EGFSNRDAGAIVLKGTTLAARAGNSPHRVYETPAGMLNAIGLQNPGVDQVVGRILGEL
                     DFTETRFIANVCGSTVEEYVEVTRRFDQSPIDAIEINISCPNVKEGGVAFGNYPDMSA
                     QVVAACRRVTRKPLITKLSPNQTDIRENARRCIEAGSDALAVINTIMGMAIDVNTRRP
                     VIGNVQGGLSGPAIKPIALLKVHQVHEVARRHGVPIIGQGGITSVADALEFIIAGASA
                     VGIGTALFYDPMICRKINTGIGEYLRKHALKSVTELVGTLRAGADPPEAPAG"
     gene            9961..11370
                     /locus_tag="VFB37_09700"
     CDS             9961..11370
                     /locus_tag="VFB37_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZO22762.1"
                     /translation="MQDKADRLQGRPAILLAVLSVARAFGCESGTDAAQPCADFRWPE
                     LLGAQYTYVRQSQTRLVSPYQGRLSLHPDGDTQSTHTIGLYLGWAMTDWAQVYLDTEK
                     FMGAGDSGAVGLGGLTNGDVVREGGVGLPKVFYIAREYLRLMLPLGPQVTQVERAQDQ
                     IGGAEAAMRLELKFGELAASDDFDHNRYAGSTRTQFMNWSLWDNTAWDYAADTRGYTY
                     GIVVGFVSPAWSLKYGAYLMPTRANGQTLENAPLRARGDNLELTLSPWSSGSVLRLLA
                     YRNTARMGDYREALAIAAMTGSTPDIVADDREGRHKYGFGINFEQPLADEGESGIFGR
                     LGWDDGRTESFAFTEVDRLASFGGQLSGRHWDRAEDHAGVAVAIEGLSAPHRDYLAAG
                     GAGFLLGDGALNYASERILEVYYRIALPWPLNGHPGFFTHYPLKMQLSPDFQYIRNPG
                     FNQDRGPVRFWGLRLHLEL"
     gene            complement(11355..12458)
                     /locus_tag="VFB37_09705"
     CDS             complement(11355..12458)
                     /locus_tag="VFB37_09705"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZO22763.1"
                     /translation="MPDWDSLLHHQFLHNELADWLVAALTFFVTLTVLPVVRGFVRAR
                     RRRSAEQGRPQVHTAIDLTMLLVSRTSRLFLLAVALYFASRDLVFAPRIERVLTIAIV
                     AVFWLQVGLWAMTAVRFAIDLRRSRSAGLDALLTSSMDVILFSAGLLVWTMVLLLALD
                     NLGVQIKPLLAGLGIGGIALALAVQTVLSDLLASMSIALDKPFGIGDFLTLGDYQGTV
                     EHIGVKSTKLRSISGEQIVIANADIVKARVRNYGRMRERRAVFQFGVNYDTPVEALAA
                     IPAEVRRIVEAQSNTRFDRCHFLTFGDIALQFELVYFVTRSEFNVYADIQQAVNLAVL
                     ERFRALRVQLAAPTRAIVYVENPGAAPPSQSSR"
     gene            complement(12557..13963)
                     /gene="ntrC"
                     /locus_tag="VFB37_09710"
     CDS             complement(12557..13963)
                     /gene="ntrC"
                     /locus_tag="VFB37_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HZO22764.1"
                     /translation="MNTHALRIWLVDDDASIRWVLERALRNGGMAPRAFEAAEPALDA
                     LRRESPDVLITDIRMPGQSGLELLKKIRSARPALPVIVMTAHSDLGSAVSAYEGGAFE
                     YLPKPFDIDQAVALVRRAAHAHPQAGIDSASTPRIPELLGRAPAMQQVFRAIGRLARS
                     SVTVLITGESGTGKELVARALHDHSPRAGKPFIALNTSAIPADLLESELFGHEKGAFT
                     GADSQRRGRFEQADGGTLFLDEIGDMSVPLQTRLLRVLAEGEFYRVGGQTPIRVDVRV
                     IAATHQNLQERVQHTLFREDLYHRLNVIRIELPPLRARAEDIPALLAHYMIVAAQELG
                     IEPKALASEAQKKLETHAWPGNVRELVNMCRRLSVLAPGSEVRLEDLPAEVMAAPAQG
                     PADADWARALAAWADREAVHARRPLLEQALPEFERVLIRCALKRTQGHRQEAAKLLGW
                     GRNTLTRKVKELGMDGEE"
     gene            complement(13960..15054)
                     /gene="glnL"
                     /locus_tag="VFB37_09715"
     CDS             complement(13960..15054)
                     /gene="glnL"
                     /locus_tag="VFB37_09715"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HZO22765.1"
                     /translation="MGVESNLPSALSHFDPADLLDALSTGIVMLDAHLCAIYANVAAQ
                     DLLAFSLKQARGRPFADLLQDSNGLRSILRRALDSGEGITDRELTVRPLGAPRDTRIL
                     DVTITPLAGQITGKHLLMELADTTQRQRISRERDLLARLDGSRLMIRQLAHEIKNPLG
                     GLRGAAQLLERELQSAALKEYTRVIIGEADRLTALVDSMAGPSRAPQKVLLNVHEICE
                     HVYHLLRAEASAAILVERDYDPSLPEAMLDRHQTIQALLNVARNAMQAISEKGRIVLR
                     TRALSNVSIGSTRHRLVASVQVEDDGRGVPVELRSSIFYPLVTGRANGSGLGLAVAQE
                     LVTRNGGFIEFESEPGHTTFTLLLPLGDSS"
     gene            complement(15097..15696)
                     /locus_tag="VFB37_09720"
     CDS             complement(15097..15696)
                     /locus_tag="VFB37_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZO22766.1"
                     /translation="MRAILFTLMSAGVLIPAAATTVYRWVDESGVTHYSDQPHENAQK
                     VELKAPQTYSAPKLPAVPSPSLPGPSQGPVYKSCQITAPSNDQMFMNTFTVTAGVSLQ
                     PPARPGDQVVVTLDGQRVPGVPPDGGAFTISPVDRGTHSLQATVQDASGRPVCQSASV
                     TFHVHQPGTLNPQTAAPAAPGVPTAPGVPTAPGVTRPRS"
     gene            complement(15823..17235)
                     /gene="glnA"
                     /locus_tag="VFB37_09725"
     CDS             complement(15823..17235)
                     /gene="glnA"
                     /locus_tag="VFB37_09725"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HZO22767.1"
                     /translation="MTTPTDVLKLIKEKDVKFADLRFTDTRGKEQHVTIPARLVDEDL
                     FRDGKMFDGSSIAGWKGINESDMILMPEAATAVLDPFFEESTVNLRCDVIEPATMQGY
                     ERDPRSIAKRAEAYLRSTGIADGALFGPENEFFIFDSVRFGNEMRGAFYSIDSVQGAW
                     NSAKEYQEGNKGHRPGIKGGYFPVPPVDGFQDIRSAMCLACEEMGLKVEVHHHEVATG
                     GQCEIGIGAGGLVTKADQVQILKYAVLNVAHGYGKTATFMPKPLVGDNGSGMHVHQSL
                     QREGRALFAGDKYGGLSDTALYYIGGIIKHAKALNAFTNAGTNSYKRLVPGFEAPVML
                     AYSARNRSASIRIPWVSSPKARRIEVRFPDSSANPYFAFTAMMMAGLDGIQNRIHPGD
                     PADKDLYDLEPEEARNIPTVCHSLDMALEHLDRDREFLTRGGVFTNDVIDAYMTLKMQ
                     EVTRFRMSTHPIELELYYSC"
     gene            17605..>18335
                     /gene="glyQ"
                     /locus_tag="VFB37_09730"
     CDS             17605..>18335
                     /gene="glyQ"
                     /locus_tag="VFB37_09730"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZO22768.1"
                     /translation="MKIGTAPGTFQELIFALQRYWSDQGCLILQPYDMEVGAGTFHTA
                     TFLRAIGPEPWSAAYLQPSRRPTDGRYGQNPFRLQHYYQYQVVIKPSPLEILDLYLGS
                     LRALGLDPLVHDVRFVEDNWESPTLGAWGLGWEVWLNGMEITQFTYFQQVGGLDCRPV
                     TGEITYGLERLAMYLQGVESIYDIVWTQTPHGPVSYGDVFHQNEVEQSTYNFELADVP
                     VLLRHFDDYEAACKGLVGAKLALPA"
BASE COUNT         3232 a         5929 c         6046 g         3128 t
ORIGIN      
        1 tgctgccagc ccattcgcat ccgccggtat tgggtgtccc actccggtgt cgggggcacg
       61 cccgagccga gcagtcgcag gatggttccg ggctccacgg gctccaggat gaagtcatcc
      121 acgatcgcgc tgtctgcgga cgggagcgac ccggagggca gatgaatgag ccgcagccgg
      181 cgtgccggct cgaatacgtc gatgtgggcc tcgagctcgg tcacccgatc aacactggca
      241 cgaaaggatc ctccgggctt cgctcggatc tgcgcgcccg gggcgcacca ccggccgagc
      301 agcgcgtctt ccgtcagcgc gcgccagacc tggctgacat cggcggcgat atcgatgcgg
      361 tgcgcatacc cgcgggcttt ctcgctcatg ggcacattct gccatccgtg acacgcgcct
      421 tgatacgctg cttgagcgta gcattggctc gtcgagcctg aggagaaccg ccatgccctg
      481 ggtccatctg gtgatcggtc ttgcgctggt cgagttcctg ttcttcggca tcgcggtcgg
      541 gcgcgcccgc gcgcgttacg gggttcatgc accggccacg accgggcacg aggtgttcga
      601 gcgctacttc cgtgtccata tgaacacgct cgagcagctc gtggtgttcg tgccctcgat
      661 cctgctcttc gcccgctatg ccaaccccgc gctggcggcc gcgctgggtg cgatcttcct
      721 ggtcggccgc ctgctgtact tcaaccgcta cgtggccgat cccagaaaac gggaacttgg
      781 atttgtcctc tcgatgctgc cgacggtgat cctgctggtg ggcgggatca tcggcgccat
      841 tcgcgcgcta ctgagcggat gacctagggc gtcggtgcgg ctcggctgcg caaccaggca
      901 acgagcaggg gcacgaggga ggcgaggatg atcagaatgg tgacgacgcc gaagttctgc
      961 ttgacgagcg ggatgttgcc gaacaggtaa ccgccccaca ggaataacat cacccacgag
     1021 acgccaccga gcaggttata gccgaggaag cgtgggtacg gcatccgccc cacgccggcg
     1081 acgaacggcg cgaaggtgcg gatgatcggc atgaagcgcg acagcgcgac ggccatcgcc
     1141 ccgtgccgcg cgaagaactc ctcggtccgg cgcagatact ccaccttcag cagcgggtag
     1201 cgcccgctga aggccggagg accgatggcg cgtccgatcg agtagttgac aatgttgccg
     1261 agcaccgccg cagcggccag cagcaggaag gccagcggcg cgctgagctt gccggtgggg
     1321 tccacggcgg cgagcgcgcc tacggcgaag agcagcgaat caccaggcag aaacggcgtg
     1381 acgaccagcc ccgtctcaca gaagatcacc gcgaacagga tcgcgtagat ccacaggtcg
     1441 tatcgggcca gcagctcgac cagatggcgg tcgagatgca gtacgagatc aatgaaccag
     1501 ccgagcaatg ccgctcacgg aacgaaagcg gcgcgcatca atgcgatacc cgtgggggga
     1561 gtgcgtgcaa cagcgccctg gaccttcagc tcgtgcaatg cgaggcgcac ttcgcgatct
     1621 cgcgagacca gggaaggggc ggacttcgcg ctgagcagtt cggcaggggg ctcctcggga
     1681 ccctcagaga cgatgtccgg cacgataccc ttgccgtgaa tcgaggcgcc ggagggcgtg
     1741 aagtagcgtg aggtcgtgag cttcacggcg ccaccgtgcg acagcggcat cacggtctga
     1801 accgagccct tgccgtaggt tttgtggccg atcagctccg ctcggccgtg atccttgagc
     1861 gcgccggcaa cgatctccga tgccgatgcc gagccgctgt tcaccagcac gacgagcggg
     1921 gcgccatcca gtacatcccc tggggtggcc tccatgctga agcgggcatc cgcagtacgg
     1981 ccatctgccg tcacgatgac tccttcgtcc aggaatgcgt ccgcgaccgc aacgccggcc
     2041 tcgagcacgc cacccggatt gtttcgcaga tcgagcacga gacccttcaa accccgtgga
     2101 ttgtcgcgtt tcagccgctc catggcgtgg ccgagatcgt ccggtgtcgt ctcgctgaag
     2161 ccgctgatcc gcacgtaacc atagcccggc tcgagatctt gctccgcaac gctgtgcact
     2221 tccaccttgg cgcggcgaag cgagaaattc agtgtttgac ccgtcgaggg ccgccggatc
     2281 gtgagcttca cgaggctgcc ggaggaaccg cgcatccgcg cgatcgcccc ggccaggtcg
     2341 gccccgatat ccatgtcatc gatccgggta atctgatcgc ccggcagcag tcccgcaagc
     2401 gcagccggcg agccctcgat gggccgcagg atcttcacag cggaatcctc ggcgacgacc
     2461 tcgatgccca cccccggata ggagcccatc gtgctcagcc ggatctcctc gaactcctcg
     2521 ctgtcgaggt aggctgaatg aggatcgagc gcagccacca tgccgcgtat ggccttgtcc
     2581 atcagcgcat gatcatcgac gtcatcgaca tactcgcgct tgatgcgctc gaagacttcc
     2641 gcgaactgac gtgcctcttc ccatggaagc gccgcgggaa cactgtcagc tttcgccgac
     2701 tggcccggac gttccgcaag aactccgctg gtcgtcgctg cggagaagcc gaacacagct
     2761 cccgcacaga gagcgatcag cgtacgcgac cgcgtagaga tcatgccctg caaacccccc
     2821 ggtttgaatg acgcacggat actacagcca tccctggtgg gtcaatcaca atccacgctg
     2881 ggatggcgct cgacacgtga cagttcccga acttgacgat cagtgcacaa tttcgacgac
     2941 acggtttatc gtcgtgagat cgctcgaggc acaaggctcg gtagcaatca gcagcggcag
     3001 ctccgcgagc agatcgatga gcggtccggg ccggccgatg gcaaagccct gaccatagtc
     3061 cacgccgagc gtcgccagtc gcgtgcggat ctcgtccgtc tcgacgtact cggccactgt
     3121 tgcgatggac atgctgcggg cgagttgcgc gatggcgcgc accatcgact ccgcacgcgg
     3181 gtccttcagg atgtctcgca cgaagctgcc atcgattttc aacatcgtga ccggcaactg
     3241 gcgcaggtac gccagcgaag acagtcccgt gccgaaatca tccagtgcga ccccgcagcc
     3301 cagccgtcgc aggcggcgga tgagcgactc cgcgcgcgcg atgttcgcga tggtggcgtt
     3361 ctcggtaagc tcgaagcaaa agagcgccgg gtccagcccg ctcgtgcgga tggcctcgag
     3421 caggaagtct acgaagtcct cgtcattgag ggactggccg gaaaagttga tggcgaagga
     3481 caccgagcgg ccggcgagca acccggcctg cggcttgagc agctcgatcg cgcgcgtgat
     3541 cacccagcga tcgatctccg gcatgagctg gtaacggttc gccgccgaca ggaaccggtc
     3601 aggcccgacg gtgcgtcctt cctcatcgac catgcgcagc agaagctcga aatgcggccg
     3661 gggactgtct gcgctggcga aggggaggat gagctgcgca tcgagcctca gccggccggc
     3721 gtcgattgcc gcgcgcagct ccccggcaat gttgatgtcc gcaaagcggc gcaccaggct
     3781 cacatcgttg gacttgtacg cctcgacacg gtttctgcca cgatccttgg cggccttgca
     3841 ggccgtttcg gcggcggcca gggaatgcat gagctcgccc gacccggtat ccagcagcgc
     3901 cacgccgatg ctgatggaaa catgcaggcg cgagtcgccg tgcgaggtgc cgagttgctc
     3961 gacaccttcg cgcagcgatt cggcaaattc ttcggcgtgg tccagatcag tgggcagcag
     4021 cacggcgaag cgatcgccgg agatgcgcgc ggcgaacgcc ccgggcggca ggcgccgccg
     4081 gatcagctcg cccagctgac cgagcaccga atctccgaca tgcatgccga agctgtcgtt
     4141 gatgacatgg agctggtcgg catcgatgta gagtgcagtc cactgccgtg atgcgcttcc
     4201 acccgccacc accgcgcgta tccgctgctc gaaagcggga cgggtataca ggccgctcag
     4261 cgcatcgaaa ctgctttcga tgataccgac cgccttcctg gcaaggatct ccgtcaaccg
     4321 cgcgtcacgc tcggagaagg cctcacccgc ctcctcgcgg aacaaggcga gcacgccgac
     4381 cgccttgccc gccacggacc gcaaggcgca cgagagaatc ttgtatggca ggacacccat
     4441 gccgctgttg ggcgcaatct tgttgatgat cacaggctcg cgacggatct gcgccatcga
     4501 caggagctga cgatgtgcgc gcgcgacgag ctgggtgtca gcaggacgct gggcgctcga
     4561 gcgcagcaac gcaatgctct tgtccggcac gatcaacgct gtggtgacgg cgcccacatg
     4621 ctcgatcgcc tgctgcagga ttcccttcag ctcatcggca ccgtcgccgg tgggacgatc
     4681 gggcgccccg tccgcgagca gcagctccag gtccttgtca cgcgaagaca ccgtgcgatg
     4741 cagctgatcg atggccgccc tcgagatcag atcgcgacgc aggcactcga gcgccggtcg
     4801 caacagagag tgggtgaacg agaatccacg tccttcggcc tcgggatcgc tgcaatgccg
     4861 gcacaggacc accacgacgc cgatcagcag ctccgagtca tcccggatcc agcagagata
     4921 gacgggctga ttgccgtcga aagtgcgcat caggcccggg ccctgcgggc ttgtgccccc
     4981 cgcggcgagt gcgctctcga cgagctgatg gagatcgggc ccagtggtcg tttcgctcga
     5041 ccatcgcagg tcgcctagcg cgtcatagag cgcaacactg gtggcgcgtg ggaggagcga
     5101 tcgcaccagc tggacgtagg gctcgaaaga gccccacgga tcgggagccg atacggtctg
     5161 gcgcagatct gtcatcccca gcggcctgga tgtccaaggg aattgggctt ggccgaacga
     5221 tctcagagca gccggcatcg gtccttgctc gagttggcga tttcagccaa atcggccctg
     5281 tcccacccgg taaaggttga ccctcatcac atctgcgcgg gccgcagcgg agggccgcgg
     5341 ctagtggcaa gggcgggcgc agtgtggcgg cgcccctagg gctgagcggc cttgaaccag
     5401 ggtcggggat cgaccgcctt gccagccttg cggatctcga aatacagctc cgggcgagag
     5461 ctgccgccgc tgtctccggc agagccgagc gtgtccccgg cgttcacgcg ctctccgacg
     5521 gccttgtaaa gcctctcatt gtggccgtac aggctcatgt agccatcgcc gtgatcgacg
     5581 atgctcagca gccccaaacc cggaagccag tcggcataga tgactctgcc gcgatagatg
     5641 gcccgcacgg gtgcgccgcg atcggttgcc accagtacgc cgtcccactt gacgccgccg
     5701 gcgcgctgct cgccgaaccg ggcaaccagc ttgccactga ccggccacgc gagcttgcct
     5761 cgcagccgtg cgaaggcgtc gttgccgttg acgggaaact gctcgatcgc acggcgcagc
     5821 tcggcgagca gcttctcgag tccgctctgt tggctcttga gacgctcgag gctctgggcg
     5881 cgcgtattcg attcggctgt cagactggcc aacacgtccg agcgccgtga ccgcgccgtc
     5941 tcgaggtcgg cgagttgcga gcgctgttgc gcctcgagtt gcgccagcct gtcgtcctcg
     6001 gattcgagct ggccgtcgag ctccgaaatc gcgtgcacgt cgttctcgat gagcgcgatc
     6061 tgctgcgccc gcgcccggcc gaagtagctg taatagacga acatgcggcc ggcgcgctcg
     6121 gggtcctgct gattcagcag cagcttcagg ggctcctcag ggcctatcag gtaggcggcg
     6181 cggagttgcg cggcgagggc accgcgattt tgagcaagat ccgcctctcg cgtgcgccga
     6241 tcggcggcca gcgaggtgcg ccgggcagcc gtcacggcgc ggttccgacg gacgctgtcg
     6301 aggccctcgc gcgccttgcc gacggactcc tcggcggagc gaagctcacg tgtcaggcgg
     6361 tcgcgctcca cctgatcctg gcgaacctcg cgcgtgatgc gctcgatttc cgccttgacc
     6421 gactgaagct gggcctcggt tctgcgggca tccgcttccg gctccttcgc aggtgcaggg
     6481 gctgccccgt ggcccggcaa gcaggccacc agggcgatca gccccacggc cagccggccg
     6541 gcccgctggt gcggcactgc aaagcgacgg cgggtcagcg taagcatagg ggcgctataa
     6601 tgcgcgccct tttttccacg ggcagccgat tgcgaaggag ccgggagcac catggaccgt
     6661 ctagtggagt atgtcggaca ccacccatgg tatgtcgcgg gagcgctcgc cgcggcgatc
     6721 ctcgtgatga tctacgagct tcgcaagcgc aaggagagct tcgccgcagt gtctccgcag
     6781 gatctggttc gccttatgaa ccagggtgcg ctcgtcatcg atctgcgccc ggcggaggcg
     6841 ttcgccagtg gccacctcgc cggcgcgcgc cacatgagcg gcgagcaggt cctcaaggcc
     6901 ggcgatactc tgaagaagca caaggaaaag acggtcgtgg tatatgacga caccggctca
     6961 ctgagcgctt cggcagtccg gcagctcgct tcccaggggt tcacgcgggc cttcaatctg
     7021 cgcgggggtc tcgccgcctg gcgggctgag aacctgccgc tctccaaggc ctaggtctgc
     7081 agcgtggggc caccggaggt actgatatac gtgacgagct ggtgcccgta ctgcgatcgg
     7141 gcacgcaggc ttctgcaaag caagggagtc ccgctgcgcg agatcaacat cgaggaggtc
     7201 ccgcagtctc gggcggagat ggtggcgcgc agcgggcgcc ataccgtccc gcagattttc
     7261 gtcggcgaga tccacgttgg agggtccgac gatctgtacg ctctcgaagc ggagggccgt
     7321 ctggacacac tactcaaatc cggagcacga aatggcgaat gaaatgaccg gtggcacgcc
     7381 gatgtccgat gcacagggtg acctcggccc catgctggcc ctgcagaacg tctacctgaa
     7441 ggactgctcc tacgaggcgc ccaacggacc tcgcgtggat ggcaactgga atccgcagat
     7501 caatctcgac ctgcatacgg gcgcaagcgg cctcacaccc gagcttcgcg aggtcgtcct
     7561 gaccgtcacc gtgtcggcga agcagaacga tgccaccgta ttcctggtgg aagtgaagca
     7621 ggcgggcgtg tttctgatgc gtaacctctc ggaggtcgat ctgaaacgcg cggtgagtag
     7681 tgtttgcccc agcgtacttt ttccatatgc gcgggccgcc atatccaacc tcgtgacaca
     7741 gggcggcttt ccacaattcc tgttgccacc ggtcaatttc gacgccctgt acgcgcagag
     7801 cgccaaccag gaccagccgg tgacgacgaa ctgacgcggc gagcggaaac ccgcggcccg
     7861 ggagttccat gagcgcagag cggcccgatt ccgcgccgaa agggcgcgca gaccttcata
     7921 cgccggtcgc ggttctgggc gccgggtcgt gggggactgc actcgcgatt cagttcgcgc
     7981 gcagtggacg gcccacgcga ctgtggggcc gcgatcgcaa gcagctgctc gccatgtcga
     8041 gcggacgctg caatgcacgg tatctcgcca gcgcgcagtt tccggccgcc ctgagcgtgg
     8101 agccggatct ggccgcggca ctctcgggca cgcgcgaagc actgattgcc gtacccagtc
     8161 atgcctttcg ggggctcttg ctgcaggtcg cgccgcatct ggccgccgac agcgcactgg
     8221 cctgggccac taaggggttc gagctggaca cgggtcagct gccgcatcag gtagcccgcg
     8281 aagtgctcgg tctgaagcgt gcgattgccg tgctgtcggg accgaccttc gcgcgtgagg
     8341 tcggctctgg cctgccgacg gccatgacga tcgcttcccc tgactcggca ttcgccaccg
     8401 cgctcgcgcg cgagctctcg tctacgaatt ttcgtgccta cacctctacg gatatcgtgg
     8461 gcgtcgaggt ggggggtgcc gtcaagaatg tgctggcagt gggggctggt ctctccgacg
     8521 ggctcggatt cggggcgaat acgcgggtcg cactcatcac acgtggtctg gtcgagatga
     8581 tgcggttggg cgtggcagcc ggagcgcgcc gcgagacctt catggggctt gcgggcctgg
     8641 gagacctggt actcacctgc acggacgatc agtcgcgtaa ccggcgcttc gggctgttgc
     8701 tcgccgccgg ccgtacggcg cccgaagcgc tggcggcgat cggacaggtg gtcgagggct
     8761 acaccgccgc cgaggcgacc ctgcgagtgg cacggcgaat gggagtcgag atgcctctct
     8821 gcgaaggagt ccatcgcgtg ctgtacgaaa gcgtgcccgc ccgggaagcc gtgcgcgatc
     8881 tgatgagccg gcccatacgc tcggaaatcg actagccgat gggacaggcg cctactcagc
     8941 ctcgggcacg ctctcggagc cttgggccat aatgtccacc atgagcagcg ctgcacctgt
     9001 cgccgatgat ggactgcgcc tgcaattctg cgggctggag tttgcctcgc ccatcgttct
     9061 gctctccgga tgcgtcggct tcggggagga gtacacgcgc atcgagggct tctcgaaccg
     9121 cgatgctgga gcgatcgtac tcaaaggcac gaccctcgcg gcccgggccg gtaactcgcc
     9181 gcatcgtgtg tacgagacgc cggccggcat gctcaatgcg atcggactgc agaatccggg
     9241 ggtggatcag gtggtgggcc gcatcctcgg cgagctggat ttcaccgaga cgcgcttcat
     9301 tgccaacgta tgcggctcga cggtcgagga gtacgtcgaa gtgacgaggc gcttcgacca
     9361 gtccccgatc gacgcgatag agatcaacat ctcctgccct aacgtgaaag agggcggagt
     9421 ggcattcggc aactatcctg acatgtctgc tcaggtggtg gcggcgtgcc ggcgcgtcac
     9481 gcgcaagccg ctcatcacca agctctcgcc caatcagacc gatatccgcg agaacgcccg
     9541 gcgctgcatc gaggcgggct cggatgccct ggcggtcatc aacacgatca tgggcatggc
     9601 gatcgatgtc aacacccgcc gcccggtgat cggcaacgtt cagggcggac tgtccggtcc
     9661 cgccatcaag ccgatagccc ttctcaaggt gcaccaggtg cacgaggtgg cgcggcggca
     9721 cggtgtgccc atcatcggcc agggcggcat cacgagcgtc gcggatgcac tcgagttcat
     9781 catcgccggc gccagcgccg tggggatcgg cacggcactg ttctacgatc cgatgatctg
     9841 ccgcaaaatc aacacgggca tcggcgagta cctgcgcaag catgcgctca agagcgtgac
     9901 ggagctggtg ggtaccctgc gggctggcgc ggaccccccc gaggcgccgg ccggctgagc
     9961 atgcaggaca aagcagaccg cctgcagggg cgccccgcaa tcctgctggc cgtgttgtcg
    10021 gtcgcgcggg cctttggctg cgagtctggg acggatgccg cgcaaccctg cgcggatttc
    10081 cgctggcccg agctgctcgg cgcgcagtac acctacgtac gtcagagtca gactcggctg
    10141 gtgtctccgt atcagggacg cctgagcctg catccggatg gcgataccca gtcaactcac
    10201 acgattggac tgtatctcgg gtgggcgatg accgactggg cgcaggtgta cctcgatacg
    10261 gagaagttca tgggggcggg cgacagcggc gcggtcggcc tcggcggcct gaccaacggc
    10321 gacgtcgtgc gtgaaggcgg ggtcggactc ccgaaggtct tctacatcgc acgcgagtat
    10381 ctgcggctga tgctgccact cggcccacag gtgactcagg tcgagcgcgc acaggatcag
    10441 atcgggggtg ccgaggccgc gatgcgcctg gaactcaagt tcggcgagct tgcggccagc
    10501 gatgacttcg atcacaaccg ctacgcgggc tcgacgcgaa cgcagtttat gaactggtcc
    10561 ctatgggata acacagcgtg ggactacgcc gctgacacgc gaggttacac gtacgggatc
    10621 gtagtcggat tcgtgagccc cgcctggtcg ctgaaatacg gcgcatacct gatgccgacg
    10681 cgagccaacg gacagacgct ggaaaacgcg ccgctgcgcg cacggggtga caatctcgaa
    10741 ctgaccctgt ccccctggag cagcggctcg gtgctgaggc tgctcgccta ccgtaacacc
    10801 gcgcgcatgg gggactatcg cgaggcgctg gcgatcgctg ccatgacggg ctcgacgccc
    10861 gacatcgtcg ctgatgaccg ggaggggcgc cataagtacg gcttcggtat caacttcgag
    10921 cagccactgg cggatgaagg cgaaagcgga atcttcggcc ggctgggctg ggacgacggg
    10981 cgaacggaga gcttcgcttt caccgaagtc gatcggctgg cgagcttcgg cggtcagctc
    11041 tccgggcgtc attgggatcg cgcggaggat cacgctggcg tggccgttgc gatcgaagga
    11101 ctctcagctc cccaccgtga ctacctcgcg gccggcggcg cgggcttcct gctcggcgat
    11161 ggcgcactca actacgcctc cgagcggatc ctcgaggtct actatcgcat cgcactgccg
    11221 tggccgctga acggccatcc gggattcttc acgcactatc ccctgaagat gcagctcagc
    11281 cccgactttc aatacatccg caaccccggc ttcaaccagg atcgcggccc ggttcgcttc
    11341 tggggcctgc gccttcacct cgagctctga gacggcggcg ccgcccccgg attctccacg
    11401 tacacgatgg cgcgggtcgg cgcggcgagc tgcacgcgca aggcacggaa gcgttccaga
    11461 accgccagat tgaccgcctg ctggatatcg gcatagacgt tgaactcgga gcgggttacg
    11521 aagtagacca gctcgaattg caacgcgatg tcgccgaatg tcaggaaatg gcagcggtcg
    11581 aaccgcgtgt tggattgggc ttcaacgatg cggcgcactt cggcgggaat cgccgccagc
    11641 gcctcgaccg gcgtgtcgta gttgaccccg aactgaaaga ctgcgcgtcg ctcacgcatg
    11701 cgtccgtagt tgcgcacccg ggctttcacg atgtcggcgt tcgcgatcac gatctgctcg
    11761 ccggagatgc tgcgcagctt ggtgctcttg actccgatgt gctccacggt gccctgatag
    11821 tcaccgagcg tgaggaagtc gccgatgccg aacggcttgt cgagcgcgat ggacatcgaa
    11881 gccagcaggt cgctcagcac ggtctgcacg gcaagcgcga gggcgatacc cccgatgccg
    11941 agacccgcca gaaggggctt gatctgcacg cccaggttgt ccagcgcgag cagcaggacc
    12001 atcgtccaca ccagcagccc ggcggaaaac aggatcacat ccatggagct ggtgagcagg
    12061 gcgtcgaggc cggccgagcg cgatctgcgc aggtcgatgg cgaagcgcac cgcagtcatc
    12121 gcccacagac cgacctgcaa ccagaagacc gcaacgatgg cgatggtcag cacacgctcg
    12181 atgcgaggcg cgaacacgag gtcgcgggaa gcgaagtaga gcgccacggc cagcaggaac
    12241 agcctgctgg tgcgggacac cagcagcatc gtgaggtcga tggcggtgtg aacctgcggg
    12301 cgcccttgtt ctgcggaacg gcggcgcctg gcgcggacga aaccccgcac gacgggcagc
    12361 acggtcagcg tgacgaagaa cgtcagcgcg gcgacgagcc agtcggccag ctcattgtga
    12421 agaaactgat ggtgcagtaa cgagtcccag tcaggcatcg gcggaattta tccagtccgc
    12481 cgcaaaaagt cgcgacttct gccgagcgat cgcggattcc gtggccggtg gggctgcgcg
    12541 cggggcatgg caacaactac tcctcaccgt ccatgcccag ctccttgacc ttgcgcgtca
    12601 gcgtgttgcg accccagccg agcagcttcg ccgcctcctg ccgatgaccc tgtgtccgct
    12661 tcagggcgca gcggatgagg acccgctcga actccggcaa agcctgctcg agcagcggcc
    12721 ggcgcgcatg aacggcctcg cggtccgccc acgccgccag ggccctggcc cagtcggcat
    12781 cggcaggccc ctgcgccggg gcggccatga cttcggcggg cagatcctcg aggcgtactt
    12841 cgctcccggg tgccaggacg gacaagcgcc tgcacatgtt caccagctcg cgcacgtttc
    12901 ccggccaggc atgagtctcg agcttcttct gcgcttccga agcaagcgcc ttgggctcga
    12961 tacccagctc ctgcgcggcc acgatcatgt agtgcgcgag cagcgccgga atgtcctccg
    13021 cccgggcgcg caggggcggc agctcgatgc ggatgacgtt caggcggtga tagaggtctt
    13081 cgcggaagag cgtgtgctgg acgcgctcct gcagattctg atgcgtggcc gcgatcactc
    13141 ggacgtccac gcggattgga gtctggcctc ccacgcgata gaactcgcct tcggccagca
    13201 cgcgcagcag gcgcgtctgc agcggcacgg acatgtcgcc gatctcatcg aggaacagcg
    13261 ttccaccatc cgcctgctcg aagcgcccgc gtcgctggga gtccgcgccc gtgaacgcgc
    13321 ccttttcgtg gccaaacagc tccgactcga gcagatcggc tggaatggcg gatgtgttga
    13381 gtgcgatgaa aggcttgccc gcacgcgggc tgtgatcgtg caacgctcgc gcgaccagct
    13441 ccttgccggt gccggactca ccggtgatca gcaccgtgac gctcgagcgg gcaagtcggc
    13501 cgatggcgcg gaacacctgc tgcatggccg gtgcgcggcc gagcagctcc gggatgcgcg
    13561 gcgtggaggc cgagtcgatc cccgcctggg gatgagcgtg cgccgcgcgc cgtaccaggg
    13621 cgactgcctg atcgatgtca aaaggcttgg gcaggtactc gaatgcgcca ccctcgtatg
    13681 cggacacggc gctgccgaga tccgaatgcg cggtcatcac gatgactggc agagcgggcc
    13741 gcgcgctgcg gatcttcttc agcagctcca atcccgactg ccccggcatg cggatgtcgg
    13801 tgatgagcac atccggcgac tcgcgccgca gagcatccag ggcgggctcg gcggcctcga
    13861 aagcgcgcgg cgccatgccg ccgtttcgca gggcgcgttc caacacccag cgtatggagg
    13921 cgtcgtcatc gacgagccag atgcgcagag catgggtgtt catgaggaat ctcccagtgg
    13981 caggagcagg gtgaaggtgg tgtgcccggg ctcactctcg aactcgatga agccgccgtt
    14041 acgcgtcacg agctcctggg cgactgcgag gccgagcccg ctgccgttcg cacgtcccgt
    14101 cacgagcgga taaaagatgc tcgagcgtag ctcgaccgga acgccgcggc cgtcgtcctc
    14161 gacctggacg ctcgccacga gccgatgtcg cgtcgagccg atgctgacat tcgacagcgc
    14221 ccgcgtgcgt agaacgatgc ggcccttctc gctgatggcc tgcatcgcat tgcgggccac
    14281 attgagcagg gcctggatcg tctgatgccg atccaacatg gcttccggca ggctcggatc
    14341 gtagtcgcgc tcgacgagga tggccgccga tgcttccgca cgcagcaggt gatagacgtg
    14401 ctcacagatc tcatgcacgt tgagcaatac tttctggggg gcgcggctgg gtcctgccat
    14461 cgagtcgacc agagcggtga gcctgtccgc ctcgccgatg atgacgcgcg tgtactcctt
    14521 gagagccgcc gactgcaact cgcgctcgag aagctgcgct gccccgcgca gaccacccag
    14581 tggatttttg atctcgtggg cgagctgccg gatcatcagg cggctgccat cgaggcgcgc
    14641 cagcaggtct cgctcgcgcg agatgcgctg ccgctgcgtc gtatccgcaa gctccatcag
    14701 cagatgcttg ccggtgatct gaccggcaag gggcgtgatc gtgacatcga ggatgcgcgt
    14761 atcacgcggc gcccctagcg gccgcaccgt caactcgcga tccgtgatgc cctcacctga
    14821 atcgagcgcc cgtcgcagga tgctgcgcag tccgttcgag tcctgcagca ggtcggcgaa
    14881 cggccggccg cgggcctgct tcaggctgaa ggcaagcagg tcctgggcag ccacgttcgc
    14941 gtagatggcg cacagatgtg catcgagcat cacgatcccg gtcgagagcg catcgagcag
    15001 gtcggccgga tcgaagtggg agagggcgga gggcaggttg gactcaacgc ccacggtatg
    15061 ccctgaggga cgatcccctg atcgtccgcg cgaggatcaa ctcctcggtc tggtgactcc
    15121 gggcgcggtc ggcaccccgg gtgcagtcgg cacgccaggt gccgccggcg cggccgtttg
    15181 cggattcaac gtcccaggct gatgcacgtg gaaagtcaca ctcgctgact gacacacggg
    15241 tcgtccactc gcatcctgca ccgtggcctg cagggaatga gtccctcgat ccacaggtga
    15301 gatcgtgaac gcgccaccat cgggcggcac gcccggaaca cgctggccat ccagggtcac
    15361 cacgacctga tcgccgggcc gggcaggcgg ttgcagactc acacccgccg tcacggtgaa
    15421 cgtgttcatg aacatctggt cgttgctggg cgcggtgatc tggcagctct tgtagaccgg
    15481 accctgcgag gggcccggta aggacggcga cggcaccgcg ggcagcttgg gcgccgagta
    15541 ggtctgaggc gccttcaact ccaccttctg ggcgttttcg tgcggctgat cgctgtagtg
    15601 cgtgacaccg ctctcgtcca cccacctgta tacggtggtg gccgccgcgg gaatcaggac
    15661 gcccgcagac attaaggtaa acaaaatggc gcgcatggca gcccagcata gcgctgccgc
    15721 atacgcgcca caaaaccgga cggcgatatc cgtgaagtac cgccaccggc gactgctgtc
    15781 ccggccgatc ccggcagctc ctccgcaact tcgatgctct cctcagcagc tgtaatagag
    15841 ctccagctcg atcggatgtg tcgacatgcg gaagcgggtg acttcctgca tcttcagggt
    15901 catgtaggca tcgatcacat cgttggtgaa cacgccaccg cgggtcagga actcgcgatc
    15961 gcgatccaga tgctccagag ccatgtcgag cgaatggcat accgttggaa tgttccgcgc
    16021 ctcctcgggc tcgaggtcat aaaggtcctt gtcggccgga tcgccgggat ggatgcggtt
    16081 ctggatgcca tcgaggcccg ccatcatcat cgccgtgaag gcgaagtaag gattggcgct
    16141 cgagtccggg aagcgcacct cgatgcggcg cgccttcggg ctcgagaccc acggaatccg
    16201 gatggaggca gagcggttgc gagcggagta ggccagcatc accggcgcct cgaaccccgg
    16261 caccaggcgc ttgtaactgt tggttccggc gttggtgaag gcgttcagcg ccttcgcgtg
    16321 tttgatgatg ccaccgatgt agtacagcgc agtgtccgag agaccgccgt acttgtcgcc
    16381 tgcaaacaga gcccggcctt cccgctgcag agactgatgc acgtgcatgc cgctgccgtt
    16441 gtcgccgacg agcggtttcg gcatgaaggt ggctgtcttg ccgtagccat gcgcgacgtt
    16501 cagcacggcg tatttcagga tctggacctg atccgccttc gtgaccaggc cgcccgcacc
    16561 gatgccgatc tcgcactggc caccagtggc gacctcgtga tgatgcacct cgaccttgag
    16621 tcccatctcc tcgcaggcga ggcacatggc cgagcgaatg tcctggaaac catccaccgg
    16681 cgggactgga aagtagccgc ccttgatgcc gggtcgatga cccttgttgc cctcctggta
    16741 ctccttggcg gagttccagg cgccctgcac ggagtcgatg gagtagaagg cgccgcgcat
    16801 ctcgttgccg aagcgcacgc tgtcgaagat gaagaactcg ttctccgggc cgaacagcgc
    16861 gccatcagcg atccccgtcg agcgcagata ggcctcggcg cgcttggcaa tggagcgcgg
    16921 gtcgcgctca tagccctgca tcgtcgcggg ctcgatgaca tcgcagcgca ggttgacggt
    16981 ggactcctcg aagaacgggt cgagcaccgc cgtcgccgcc tcgggcatga gaatcatgtc
    17041 cgactcgttg atgcccttcc acccggcgat cgaggagcca tcgaacatct tcccgtcgcg
    17101 gaacagatcc tcatccacca gccgcgcggg gatcgtcacg tgctgctcct tgccgcgcgt
    17161 gtcggtgaag cgcaggtccg caaacttgac gtctttttct tttatgagct tgagaacatc
    17221 ggtgggtgtc gtcatgtcgc ctccggggaa gtggtggtgc ggacggcgtg tgcaggcgcc
    17281 gtgccagagc gggcggacga gaaatcaagc gcttactcgc cccaggactg tgcgtcgctc
    17341 catattagtg catcaaggcg cccggttgca ccagtgaggt gcagtgagag gttggtaaag
    17401 gcggcgatgg gcctcgctgg ccgtggcctg aggcgccggc ggtttcctgg ccgcaccggt
    17461 tgggtgcagt cgcctgacac tggcgaccgc gagagcctca gcgcgaagtg atcgggcccg
    17521 ggcacgctgt cgttatactc gctcgcgcgg aaggcgcctc cggcgcgttc cgcgccgttt
    17581 tcaatttcaa tcgcgagtcc ccgcatgaag atcggcaccg cgccgggcac ttttcaggag
    17641 ctcatttttg ccctgcagcg ttactggtcg gatcagggtt gcctgatcct gcagccttac
    17701 gacatggaag tgggtgccgg cacgttccat acggcgacct tcctgcgcgc gatcggacct
    17761 gagccctgga gcgcggcgta cctgcagccc tcgcgccgcc caacggatgg ccgctacggc
    17821 cagaatccct tccgtcttca gcactactac cagtaccagg tcgtgatcaa accctcaccg
    17881 ctggagatcc tggacctgta cctcggcagc ctgcgcgcgc taggcctgga tcccctggtt
    17941 catgacgtcc gtttcgtgga agacaactgg gagtctccta cgctgggcgc ctggggcctc
    18001 ggctgggaag tgtggctcaa cgggatggag atcacccagt tcacctactt ccagcaggtc
    18061 ggtggtctgg attgccgccc ggtgaccggc gagatcactt acggcctcga gcgactggcc
    18121 atgtatctgc agggggtcga gagcatctat gacatcgtct ggacgcagac ccctcacggc
    18181 cctgtgagtt acggcgacgt gtttcaccag aacgaggtcg agcaatcgac ctacaacttc
    18241 gagctcgccg acgtccccgt gctgcttcga catttcgatg attacgaggc ggcttgtaag
    18301 gggctcgtcg gggcgaagct tgcgttgcca gccta
//
LOCUS       DASRHN010000243        18481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_245324, whole genome shotgun sequence.
ACCESSION   DASRHN010000243 DASRHN010000000
VERSION     DASRHN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18481
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245324"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..1007
                     /locus_tag="VFB37_09735"
     CDS             423..1007
                     /locus_tag="VFB37_09735"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HZO22769.1"
                     /translation="MLTRKRTSSVQTQREPGQATAGAHERLWMTASASMVFILLLQIV
                     HHYRNELAANTHLNRPLTAVYSALGIRLIPRWNLGAYEVRQLGASTAPDAPGQITVRA
                     SVKNDAGQSQPLPLLRVTLQDRFGNRIAARDVPPRAYLPGAPHENAFLSAGQRVDAEM
                     AFVDPGANAVGFEIDACLPAPGGVIACANDTLSR"
     gene            1021..2025
                     /gene="dusB"
                     /locus_tag="VFB37_09740"
     CDS             1021..2025
                     /gene="dusB"
                     /locus_tag="VFB37_09740"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZO22770.1"
                     /translation="MIRIGTYALPASAVLAPMAGVTDRPFRILCRKFGAGLAASEMVT
                     SDVRLWNTPKSRLRMSHEGEPEPRVVQLAGADGQALAEAARLNVDLGAQIIDLNMGCP
                     AKKVCGKLCGSALLQDESLVGRILDAVVNAVRVPVTLKIRTGWNRESRNGVRIARLAQ
                     DAGIQALAVHGRTRADFYEGSAEYETIRDIKAAVQIPVLANGDVATARKAREVLDFTG
                     ADGVMLGRAAQGAPWIFRFVNSWVKHGIDAAPLSRAELRDIILGHLDSLYAFYGEVSG
                     VRIARKHLGWYCGQLLEDAAPVRRSLLAAATTAAQFAQASEFLRSWVEQGSVLDGDLK
                     "
     gene            2022..2318
                     /gene="fis"
                     /locus_tag="VFB37_09745"
     CDS             2022..2318
                     /gene="fis"
                     /locus_tag="VFB37_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HZO22771.1"
                     /translation="MTAKKKTRARGESSARLSGRELPLRNHAERALSDYFTSLNGHRP
                     AHLYDLVLREVEEPLFRAVLDYAQGNQSRAAGILGINRGTLRKKLKQFGLAGDA"
     gene            2396..3961
                     /gene="purH"
                     /locus_tag="VFB37_09750"
     CDS             2396..3961
                     /gene="purH"
                     /locus_tag="VFB37_09750"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZO22772.1"
                     /translation="MLLPVRRALLSVSDKTGLLELARTLAARHIEILSTGGTARLLAA
                     NGVSVREVSAYTGFPEIMDGRVKTLHPKIHGGLLGRRGVDEGVMALYQIEPIDLLVVN
                     LYPFAETVARPNCTQAEAVENIDIGGPAMLRAAAKNHEGVAVVVDPADYAMLLDELRA
                     HEGATTLAHRSRLAAKAFAHTARYDTMVASYLSAAHPGPTEHFPATLPLVFDKVQDLR
                     YGENPHQLAAFYRDPASREASIASARVLQGKDLSFNNVADADTAIECMRQFEEPACVI
                     VKHANPCGVSVAATALEAYDRAYRTDPTSAFGGIIALNRELDAATAAAIVERQFVEVL
                     AAPAVSPEAIRVLSGRPNVRVLALGELRGAAPGGLEYRSVTGGLLAQTRDAGRVVAAD
                     LKVVTRRAPTPAEVADLLFAWRVCKFVKSNAIVFARRGSTLGVGAGQMSRIYSTRLAA
                     MKAADENLELPGAVMASDAFFPFRDGIDVAAEYGIRAVIQPGGSRNDPEITAAADEHG
                     MAMVFTGVRHFRH"
     gene            3972..5261
                     /gene="purD"
                     /locus_tag="VFB37_09755"
     CDS             3972..5261
                     /gene="purD"
                     /locus_tag="VFB37_09755"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZO22773.1"
                     /translation="MRILIIGSGGREHALAWKCASSPRVSEVLVAPGNAGTATEPRVR
                     NAQVQADDFAGLIGLARAEHIDLTIIGPEGPLVAGVVDAFEAAGLKCFGPRRACAQLE
                     GSKAYCKEFLQRHRIPTASYRTFTRASFDPAYVQRQRTPVVVKASGLAAGKGVVTAET
                     AAEAIRAAEAMFAGEFGAAGEEVVVEEFLAGEEASFIVMADGRHILPLATSQDHKRLL
                     DADQGPNTGGMGAYSPAPIVTEALHARIMREVIEPTIRGLAAEGMPYTGFLYAGIMVA
                     ADGTPNVLEFNCRFGDPETQPILMRLESDLTELCEAAVAGRLETVRAAWDSRAALGVV
                     MAAAGYPKEVRTGDVVNGLEAAARLPGKIFHAGTRLKDGRVVTSGGRVLCAAGLGGSV
                     REAQRQAYALVDVVSWPGVQYRRDVGFRAVAREPTQR"
     gene            5242..5724
                     /locus_tag="VFB37_09760"
     CDS             5242..5724
                     /locus_tag="VFB37_09760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZO22774.1"
                     /translation="MSPRSADWDFETPHVLGLQVQAGDIDAYGHVNNSIYLAWFDRVA
                     WSHSAALGISLDDCVRQRRGMAAHRTEVDYLRAAVLGERVLIGTWIVASDGRLRVERR
                     FQVRRAEQVHAHITKVGETMARARTEYVCINLDSGRAVRMPESFSRAYRLTVAPAEKQ
                     "
     gene            6011..6253
                     /locus_tag="VFB37_09765"
     CDS             6011..6253
                     /locus_tag="VFB37_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22775.1"
                     /translation="MMMLGGVDGTSSWLETQYCASVAAALLARAEYFELLEDACVSPG
                     TLSRALRTWRNHVSQAEALARFPMSADLPTSGDWLA"
     gene            complement(6250..7101)
                     /locus_tag="VFB37_09770"
     CDS             complement(6250..7101)
                     /locus_tag="VFB37_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22776.1"
                     /translation="MRMRPTIVGALFALAAVAAHAERDPLTGAPLPPGHKRDTPSPIT
                     DRFSIRGTFFDPSFNTTLRVDSKTNGTMGTPVSAENDLGMPARRPQGRMELMFRLRKR
                     NKLRVDYFETSRSGTQVLARQIAFGDETFNAGDLATSSLDWRSFNLTYTYSVIRTDRL
                     EIGTGLGLHLLEAQAQGAVPARQLQQDVSGAGAFPTIPLELIWRISRRFALTARGQYF
                     HIAHNGSAGSLGDYHADVQYRWAPNFAIGAGYSELRYMLEVNDASFPGSFRMNVRGPE
                     AFVRVSF"
     gene            complement(7377..8633)
                     /gene="rho"
                     /locus_tag="VFB37_09775"
     CDS             complement(7377..8633)
                     /gene="rho"
                     /locus_tag="VFB37_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZO22777.1"
                     /translation="MNLTELKSRPIHELVKLAESMGLENLARSRKQDIIFSILKAHAR
                     NGENIYGDGVLEILQDGFGFLRSADGSYLAGPDDIYVSPSQIRRFNLRTGDTISGLIR
                     PPKDGERYFALLKVGEINFDSPDSSRNKVLFENLTPFHPTKRLKLERGNGSTEDLTAR
                     TIDLVAPIGKGQRGLIVSPPKAGKTMLLQNIATAITSNHPEVYLIVLLIDERPEEVTE
                     MQRTVKGEVVSSTFDEPAARHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTILATALIDTGSKMDDVIYEE
                     FKGTGNSEIHLDRRISEKRVFPAVNINRSGTRKEELITDQGELAKMWILRKLLHPMDE
                     LAAIEFLLDKMKDTKSNGEFFEAMKR"
     gene            complement(8822..9178)
                     /gene="trxA"
                     /locus_tag="VFB37_09780"
     CDS             complement(8822..9178)
                     /gene="trxA"
                     /locus_tag="VFB37_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HZO22778.1"
                     /translation="MSSPHIVHTTDATFSQDVLKSDKPVLLDFWAEWCGPCKMIAPIL
                     DEIANEYQDRIKIAKLNIDENPQTPPKFGIRGIPTLILFKNGTVEAQKVGAVSKSQLA
                     AFLDSNLDAAAALARK"
     gene            9470..10768
                     /locus_tag="VFB37_09785"
     CDS             9470..10768
                     /locus_tag="VFB37_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO22779.1"
                     /translation="MTDPHSGSPEFASLGLAPPVMEGIRAAGFVTCTPIQAQTLPIAL
                     SGQDVAGQAQTGTGKTAAFLVALYQALLTRPRPAGHGRASIRALIVAPTRELAVQIHH
                     DAETLGRFTGLRQAVVFGGVDYDKQRRQLEEGVDVLIGTPGRLIDYFKQRVFELRHAQ
                     VVVLDEADRMFDLGFIADIRYLLRRLPHPEHRQSMLFSATLSHRVLELAYEHMNNPEL
                     VRIEPDKMTVDQVRQLMYYPAMDEKLPLLVGLLRQYEARRTMIFVNTKRAAERLESTL
                     RANGFHAQALSGDVPQNKRLKFLRDFHNGDLAVLIATDVASRGLHIPEVSHVFNFDLP
                     QDSADYVHRIGRTARAGAEGDAISFACEEYAVSLPDIEEYIGHKIPSMPIAPELLATA
                     VAAAPAAERHRPRHVRPGGHSSGRRPPPRRHGKPPRGRGR"
     gene            10928..11152
                     /locus_tag="VFB37_09790"
     CDS             10928..11152
                     /locus_tag="VFB37_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HZO22780.1"
                     /translation="MAEIEDNVDRELFRNIEKLRQLRIEHRDLDEVIERLTMDLHTNE
                     LQLKRLKKRKLMLKDQITRMESQLIPDLNA"
     gene            11149..12519
                     /locus_tag="VFB37_09795"
     CDS             11149..12519
                     /locus_tag="VFB37_09795"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZO22781.1"
                     /translation="MNHVDSADGALMGAFLRLTSPQILTEVIAIACAGLIALAGARIV
                     RAWHKRHTTDGRGEGWQADVQEGTIIVAPYLVALVVLLLVRSILSFLHAHTSAIDTAL
                     QLTTALVLVRLGVYLLRVMMGPDSWIRTWENRITLVVWLTMSFSLVGWLDAVENTLNH
                     INLIPGKTQFSLWALLKGLVVVSAFVVVTSLIARAFEKRVMRLPQLAVSTRIGISKFA
                     YFILVGIGVMLGINEAGVDVTTLNVLTGAIGLGLGFGLQSIASNFVSGFVLLMDKSIK
                     PGDVISFTGHTGTSTENFGWVQELRGRYVVVRDRDGVETLVPNQNLITNSVINWSYSD
                     QRVRIRLPVMISYEDDPEFALKLLLEAVAHHSRILREPPPVTRLLAFEDYGMRIEVRF
                     WIADPMNGVNNVRSDINRLIWKLFREHGVKMPVAQREFRMLDPVRSTQIPSRPLPTPP
                     EESVGD"
     gene            12540..12965
                     /gene="arfB"
                     /locus_tag="VFB37_09800"
     CDS             12540..12965
                     /gene="arfB"
                     /locus_tag="VFB37_09800"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZO22782.1"
                     /translation="MPLEIAPDVVIADSELEVSFVRSSGPGGQNVNKVASAVQLRFDL
                     AASSALRPEVKARLRALAGRRLTDEGALLIIARNHRTQEQNRREALERLKDLVVRALV
                     APKPRRPTKPTRASRQRRLDSKAHRRTTKGLRAKVHWES"
     gene            12970..13431
                     /locus_tag="VFB37_09805"
     CDS             12970..13431
                     /locus_tag="VFB37_09805"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HZO22783.1"
                     /translation="MDLMPALMAFVHHLAAFTLVGALAAEVVLFKPPLTVPQARRVQR
                     ADLVFGAAATLVLVVGLLRVFFFEKGAGYYFSNVFFLTKLSLFILAGLISIYPTVLFL
                     SWSKTLRQGNTPTIAPEQVRRARMCMMLELTAILGILICAPLMARGFGVMG"
     gene            complement(13428..14852)
                     /locus_tag="VFB37_09810"
     CDS             complement(13428..14852)
                     /locus_tag="VFB37_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZO22784.1"
                     /translation="MNIEEFRRVGHELIDWIADYRERAGRGEFPVTPRMSPGSLKRAL
                     PKSPPLEPQPSDVLLHDLDELILPACTHWLDPRFFAYFPSNSSLAAVLADQVSTGLGQ
                     LGLNWQASPALTELEEVAIDWLRQMLGLSESWSGVIQDTASSGTLVGLLCARERASDY
                     GAGRGGLQGEAKALVVYASSQSHSSVDKAAYMAGFGRVNLHAVPVDEHFAMRPEALEE
                     AVRTDLSNGLKPCAVVATTGTTATTAIDPLAGIAKVAQTHALWMHVDAALAGSAMILP
                     ECRTLWEGIEGADSLVINPHKWLGASFDCSTYFVRDPQHLVRVMSTNPSYLRTADDAQ
                     VKNFRDWGIPLGRRFRALKLWFLIREQGVSGLQARLRRDIDYARWFAARVDGTPGWKR
                     LAPVPLQTVCVRHEPVGLAGEALDAHTLGWVRRINESGQAYLTPAILDGRWMVRVSFG
                     NEATQQHHVAELWQLMQREAARQD"
     gene            complement(14881..15888)
                     /locus_tag="VFB37_09815"
     CDS             complement(14881..15888)
                     /locus_tag="VFB37_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZO22785.1"
                     /translation="MSHTPASGPGLLVVGREDVRRALPMADCITAVELAMRAFSRGDA
                     NIPLRTVFALPQGRRFFGVMSGYLGEPRGLGAKILTVYPENAQRGLPSHVGVVVLFDA
                     EMGHPLAVMDAGMITALRTAAATAVATRALAREDADRLAILGTGEQAVTHLEALALVR
                     PLRSVSVWGRTSAKARKFADEQSARFSLPIEARETADEAVDGADIVCTVTAAREPILR
                     GAALARGTHVNLVGSSRLPAREADDEVVARSRFFVDSKTSAAAEAAELRHAQEAGLVT
                     EGHVLGEIGEVLSGRVPGRTGDREITVYKSHGIAAQDLAAANAIYERAVRDGIGTRVP
                     F"
     gene            complement(15930..18365)
                     /gene="plsB"
                     /locus_tag="VFB37_09820"
     CDS             complement(15930..18365)
                     /gene="plsB"
                     /locus_tag="VFB37_09820"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HZO22786.1"
                     /translation="MNWLLRRLLALWVRFKIRPDEAAAHLRERDRPVCYALERRSAAD
                     LAVLQNACVRLKLARPRKRLMPATRDLRSFFYLSRPRGFWDERIDRRPPPQLQQMIAA
                     LAHDPHLDVDLVPVSVYWGRAPQKEASWFRLLLVEEWALASRMRKLMQVLFNGRNTLI
                     EFDEPVSLRSLLGDEAGTAIRGRRVARALRVLYARQRSARIGPDLSHRRTILNRVLRT
                     RAVRAAVAQEMREKGLDRRQAMLKARQYAGEIAANYSHAFIRFMEAALSRLWNRLYDG
                     VVFGHVETLEEVAEGNEIIYVPCHRSHMDYLLLSYAIYVHGFAIPHIAAGINLNLPIV
                     GRFLRKGGAFFIRRSFRGNALYTVVFMKYLAAIMARGHSIEYFIEGGRSRTGRLLQPK
                     TGMLSMTVRSFLRDPGRPVVFIPVYFGYERIVEGSTYIGELSGKPKEKETVFGLIRTL
                     RCMRERFGQVHVNLGEPIHLAELLERYDPDWRGRPVEDETRVPWIGAAVDDLAGTIMR
                     HINAAAAVTPINLLAITLLAMPRQRLPKPELLRQLDLYRDLLRGFAYSDRVTVTSHTP
                     AQILAYGESMKVVSREPHPLGDIIRMSDESAVLATYFRNNILHLFAMPSLVACAFVSN
                     ARVRTEDIQRLAWRIYPYIAAELFLRWNERELPDVVNEVLACLELHGLVEGDPDRTEW
                     RRPPPDSTKAMQLSLLAQATIQTIERYYLAVAQLVKAGSGTISQRVLEERCQLTAQRM
                     TLLYGLNSPEFFDRTMFENFIDLLRARGVIRLSASGKLEFDEVLLRVAADAQFVLSEQ
                     IRHSILQVTGLDT"
BASE COUNT         3212 a         6029 c         6009 g         3231 t
ORIGIN      
        1 gcgcttccct ggagccttac tcaacaccgg actcccagcg ccgggtcccg gccgatgcca
       61 tgtttccgac gatggcaaag acgccggccg cagcctctcc agcaccgcca atgtcgccgg
      121 ccaaaataac atcgatgccg aaggctccgg cgggtgcgag tccggcgctg catactcccg
      181 cggctcagga gccatcgcag acgccgatgg cggcgcgcgc cagggcaaga gccgaggcgg
      241 cacgtgcgcc agcagccctg gcggcgcgtt cgccaacagg cccggcacgt ccgcctgcag
      301 ctgagtcggc acgtccagcc gcagccgagt ccgcacgtcc gcctgcagct gagtcggcac
      361 gtccagccgc agccgagtcc gcgcgtccgt cttcgttcga gtcagcacag gccatccgct
      421 cgatgctgac ccgcaagcgc acgtcctcgg tgcagacgca gcgggagccc gggcaggcca
      481 ccgcgggcgc acacgaaagg ctgtggatga ccgcaagcgc ttccatggtt ttcatcctgc
      541 tgctgcagat cgtgcaccac tacaggaacg agctggcggc caatacccac ctcaaccgtc
      601 ccctcactgc agtgtattcg gcgctcggca tccgactgat tccgcgttgg aatctcggcg
      661 cttacgaagt gcgccagctc ggcgcctcta ccgcacccga tgcgccaggc cagatcaccg
      721 tgcgcgcaag tgtcaagaac gatgccggcc agtcacagcc cctgcccctg ctgcgcgtga
      781 cgctgcagga tcgcttcggt aaccgcatcg cggcacggga cgtgccgcca cgagcttacc
      841 tgccgggagc gccgcatgag aacgcgttcc tgtccgcagg acagcgggtc gatgccgaga
      901 tggcattcgt cgatccgggc gcgaacgcgg tcggattcga gatcgacgcc tgcctgcccg
      961 ctccgggcgg cgtgatcgcc tgcgcgaacg acacgctctc gcgctgattc gctcgaccgc
     1021 atgatccgga tcggaacgta cgcccttcca gcttctgccg tgctcgctcc catggccggc
     1081 gtcaccgacc gtccgtttcg catcctgtgt cgaaagttcg gcgcaggact tgccgcatcg
     1141 gagatggtga cctccgacgt tcggctgtgg aacacaccga agtcgcgttt gcgaatgagc
     1201 cacgagggcg agcccgagcc gcgtgtcgtg caactggccg gggcggacgg gcaagccctc
     1261 gcggaagctg cgcgcctcaa cgtggatctc ggcgcgcaga tcatcgacct gaacatgggc
     1321 tgccccgcca agaaggtctg cggcaagctg tgcggatccg cgctgctgca ggacgaatcg
     1381 ctcgtggggc gcatcctcga tgcggtggtg aacgccgtgc gtgtgcctgt cacgctgaag
     1441 atccgcaccg gatggaaccg cgagagccgt aacggcgtga gaatcgcgcg cctcgcgcag
     1501 gatgcgggca tccaggcgct cgccgttcac ggccgtacgc gcgcggactt ttatgaaggc
     1561 agcgccgaat acgagaccat ccgcgacatc aaggccgccg tgcagattcc ggtgctcgcc
     1621 aacggcgatg tcgccacagc gcgcaaggcg cgcgaggtcc ttgatttcac gggcgccgat
     1681 ggagtaatgc tcggccgcgc ggctcaaggg gcaccctgga tttttcggtt cgtcaactcc
     1741 tgggtgaagc acggaattga cgcagctccg ctctcgcgcg cggagctgcg tgatatcatc
     1801 ctcgggcatc tcgactccct ctacgctttc tacggcgaag tcagcggagt ccggatcgca
     1861 cgcaaacatc tcggctggta ttgtggacag ttgctcgagg atgcagcgcc ggttcgccgc
     1921 agtctgttgg ctgctgcgac gacggccgca cagtttgcgc aggcgagcga attcctgcgc
     1981 agctgggtgg agcaaggttc agttcttgat ggggacctga aatgactgca aaaaagaaga
     2041 ctcgtgcgag gggggagtcc agcgcccgat tgagcggcag ggaactgccg ctgcgcaatc
     2101 acgccgagcg ggcactgagc gactacttca cgagcctgaa cggccaccgg cccgcccatc
     2161 tctacgatct cgtgctgcgt gaggtcgagg agccgctgtt tcgtgcggtg ctcgactacg
     2221 cccagggcaa ccagagccgc gcggccggaa tcctcggcat caatcgcggt acgctgcgca
     2281 agaaactcaa gcagtttggg ctcgccggtg atgcctagtc gctgactgcc ccgacatggg
     2341 agccctgtgc gtccgagcgg acgcgcgcgc tcgctccgcc tcgtaaggct cgcagatgtt
     2401 gttgcccgtt cgccgcgcgc ttctctccgt ttccgacaag accggtttgc tcgagctggc
     2461 gcgcaccctg gcggcgcgcc acatcgagat cctctccacc ggcggtaccg ctcgcctgct
     2521 cgccgccaac ggggtgtcgg tccgagaggt ttccgcctac accggatttc ccgagatcat
     2581 ggacggtcgc gtcaagacgc tgcacccgaa aattcacggc gggctgctcg gccgccgcgg
     2641 agtggacgag ggcgtgatgg cgctgtatca gatcgagccg atcgacctgc tggtggtgaa
     2701 cctctacccg tttgccgaga ccgtggcgcg gcccaattgc actcaggcgg aagcggtgga
     2761 gaacatcgat atcggcggcc cggccatgct gcgtgcggca gccaagaatc acgaaggtgt
     2821 ggccgtggtg gtggatcccg ccgattacgc catgctgctc gatgagttgc gcgcccacga
     2881 gggcgccacc acgctcgccc accgatcgcg cctcgcggcc aaggcgttcg cgcacaccgc
     2941 gcgctacgac acaatggtcg caagctacct gagcgccgct caccccgggc cgacggaaca
     3001 cttccccgcg actctgccgc tggtctttga caaggtgcag gacctgcgct acggcgagaa
     3061 tccgcaccag ctcgccgcct tctaccgcga tcctgcctcg cgcgaagcca gcatcgcctc
     3121 ggcgcgtgtg ctgcagggca aggatctgtc cttcaacaat gtcgcggacg ccgatacggc
     3181 catcgagtgc atgcgccagt tcgaagagcc ggcctgcgtg atcgtgaagc acgccaatcc
     3241 gtgcggcgtg tcggtagccg cgacggcgct cgaagcctac gaccgcgcgt accgcaccga
     3301 cccgacatcc gctttcggcg gcatcatcgc gctcaaccga gagctcgatg cggcgaccgc
     3361 tgcagcgatc gtcgagcggc agttcgtgga agtgctggcg gcgcctgcgg tttcgcccga
     3421 agcgatacgc gtgctctcgg gaagacccaa tgtgcgcgtg cttgccttgg gagagctgcg
     3481 cggcgctgcg cccggcgggc tcgaataccg cagtgtcacc ggaggtctcc tcgcacagac
     3541 gcgagatgcc gggcgcgtcg tggccgcgga cctgaaagtc gtgacccgac gcgcgcccac
     3601 acccgccgaa gtggctgatc tgctgttcgc ctggcgcgtg tgcaagttcg tgaaatcgaa
     3661 cgcgatcgtg ttcgcaagac gcgggagcac cctaggggtg ggagcgggcc agatgagtcg
     3721 tatctattcg acacggctcg cggccatgaa agcggcggat gagaatctcg agctgccggg
     3781 ggctgtcatg gcctcggatg ccttcttccc tttccgggac ggcattgacg tggccgccga
     3841 gtacggcatc cgggcggtga ttcaacctgg cggctcgcgc aatgaccccg aaatcacggc
     3901 ggcggcggac gagcacggca tggccatggt gttcaccggc gttcgacact tccggcactg
     3961 atgacggctc gatgagaata ctgatcatcg gatccggcgg ccgggagcat gcgctcgcct
     4021 ggaaatgcgc ctcatcgccc cgcgtcagcg aagtcctggt cgcaccgggc aatgcaggta
     4081 cagccacgga gccacgcgtc cgcaacgcac aggttcaggc ggatgatttc gcgggtctca
     4141 tcgggctcgc ccgcgccgag cacatcgatc tgaccatcat cggtcccgag gggccgctcg
     4201 ttgcgggcgt ggtggacgcg ttcgaggcag ccggcctcaa atgcttcggg ccgcgccgcg
     4261 catgcgcgca gctcgagggc tcgaaggcgt actgcaagga gttcctgcag cgccatcgca
     4321 ttcccaccgc gagctatcgc accttcacgc gcgccagctt cgatccggcg tacgtgcagc
     4381 gccagcgcac cccggtcgtc gtgaaagcga gcggactggc cgccggcaag ggcgtggtca
     4441 ccgctgagac ggccgccgaa gccatccggg ccgccgaggc gatgttcgcc ggtgagttcg
     4501 gcgcagcagg tgaagaggtc gtggtcgagg agtttcttgc cggagaggag gccagtttca
     4561 tcgtgatggc cgatggccgg catatccttc cgctcgccac gtcccaggat cacaaacgct
     4621 tgctcgatgc cgatcagggc ccgaacacgg gcgggatggg cgcctattca ccggctccga
     4681 tcgtgacgga ggccctgcat gcccgcatca tgcgtgaagt catcgagccc accatccggg
     4741 gactggccgc cgagggcatg ccctacaccg gatttctcta tgccggcatc atggttgccg
     4801 cggacggcac gcctaacgtg ctggagttca attgccgctt cggtgatccg gagacacagc
     4861 cgattctgat gcgcctcgaa agtgacctca cggagctgtg cgaggccgcc gtggcaggcc
     4921 ggctcgaaac ggtgagggca gcgtgggatt cacgcgctgc cctgggtgtg gtgatggccg
     4981 cggccggcta tccgaaggag gtgcgcacgg gagatgtcgt caacggcctc gaggctgctg
     5041 cccgcctgcc cggcaagata tttcacgccg gcacgcggct gaaggacggt agggtcgtga
     5101 ccagcggggg acgcgttttg tgcgccgcag ggctcggcgg cagcgtgcgg gaggcacaga
     5161 ggcaggctta cgccctggtc gatgtggtga gctggcccgg agtccagtat cggcgtgatg
     5221 ttggtttcag agccgtggcc cgtgagccca cgcagcgctg actgggactt cgagacccct
     5281 catgtgctgg ggctgcaggt gcaggccggc gacatcgatg cctacggtca cgtcaacaat
     5341 tcgatctacc tggcctggtt cgatcgagtc gcctggtcac actcggccgc cctcgggatc
     5401 tcactcgatg attgcgtgcg ccagcgccgc ggcatggccg cccaccgcac ggaggtggac
     5461 tatctgcgcg cggcggtcct gggagagcgc gtgttgatcg ggacgtggat cgtggcttcc
     5521 gacggcaggc tgcgggtcga gcgcagattc caggtgcgac gggccgagca ggttcacgcc
     5581 catatcacga aagtggggga gacgatggcc cgtgctcgca ccgagtacgt ttgcatcaac
     5641 ctcgatagcg ggcgtgccgt tcgcatgccg gagtcattct cccgggctta cagactcacg
     5701 gtcgcgcccg ctgagaagca gtgacgcgcc cgggcaggcg gcggccgtaa ctccctgggc
     5761 ctgccgatct gcaggttgca ttgcgcgggc gacccgcgaa actcgactgt aataaataca
     5821 gctccttgtc agcaaagtgg tccaggatgg gtcggctggc cgtgcagtgc caccatgctc
     5881 ccgcaccgcg accgcgcggc ccaggaacac cgattgcaag gaaagtccca gctcgagttg
     5941 ggtgaccgca ggcaggcgaa cgcctacgga aaaatcagac ggacgcctga agacatgagg
     6001 agatcgaaca atgatgatgt tgggcggcgt tgacggcacc tcctcctggc tcgagacgca
     6061 atactgcgca tcggttgccg cagctctgct ggcgcgtgcc gagtacttcg agctgctgga
     6121 ggatgcctgc gtttctccgg gcaccctgag ccgtgcattg cgcacttggc gcaatcacgt
     6181 gtcccaggct gaggctttgg cgcgatttcc gatgtcggcg gacctgccga cctcgggaga
     6241 ctggctggct tagaagctga cgcgaacgaa ggcttccgga ccgcggacgt tcatcctgaa
     6301 gctgcccggg aaacttgcat cgttcacctc gagcatgtag cgcagctcgg agtagcccgc
     6361 gccgatggca aagttgggag cccatcggta ttgcacatcc gcatggtaat cgcccaggga
     6421 ccctgccgag ccgttgtggg cgatatggaa gtattgcccg cgcgcggtga gcgcaaaacg
     6481 ccgggagatc cgccagatca gctcgagcgg aatcgtcggg aacgctcctg cgcccgagac
     6541 gtcctgctgc agctgccggg cgggcacggc cccttgcgcc tgcgcttcca gcagatgcaa
     6601 ccccaggccg gtgccgatct cgagccgatc ggttcgaatg actgagtacg tgtaggtcag
     6661 gttgaaggag cgccagtcga gcgagctggt cgccagatcg ccggcattga aggtctcgtc
     6721 cccgaaggcg atctgccgcg cgagcacctg agtgccgctg cggctggtct cgaagtaatc
     6781 cacgcgcagc ttgttgcgct tgcgcagccg gaacatcagc tccatccggc cctgcggccg
     6841 gcgggccggc atgccgaggt cgttctcggc actcaccgga gtacccatcg tgccgttggt
     6901 tttggagtcc acccgcagag tcgtattgaa cgaggggtcg aagaacgtac cgcggatcga
     6961 gaatcggtcg gtaatgggac tcggcgtgtc gcgcttgtgc ccggggggaa gcggcgcgcc
     7021 ggtcagcggg tcacgctcag catgggcggc aacggcggcg agcgcgaaca gcgccccgac
     7081 gatggtgggc ctcatgcgca tacgactcgg gctcctcaag gcaactggcg cggcgcgaat
     7141 cttactgatt caacgacgga tcatgccagc ctcggcgccc cccaactgaa gaccaaccca
     7201 cagaaatcag tgaccaccgc cggcgccctg gccgcccgtc gccgatcagc aacactcctg
     7261 actcgccggg gggagcacaa gtcacgactc gagggtcgca cggtcacgcg acccgcaggt
     7321 gggtttcgat cctcagagga ggatcgagtc ggctggtttt ccgcggcaac cgccgctcag
     7381 cgcttcatgg cctcgaagaa ctccccgttg gacttggtgt ccttcatctt gtcgagcagg
     7441 aactcgatcg ccgcgagctc atccatcgga tgcagcagct tccgcaggat ccacatcttg
     7501 gcgagctcgc cctgatcggt gatgagctcc tccttgcggg tgccggaacg gttgatgttg
     7561 actgccggga agacgcgctt ctcggaaatg cgccgatcca ggtggatttc gctgttgccg
     7621 gtgcccttga actcctcgta gatcacatcg tccatcttcg agcccgtgtc gatgagcgcg
     7681 gtagccagaa tagtgaggct gccaccctcc tcgatgttgc gcgcagctcc gaaaaagcgc
     7741 tttggacgct gcagcgcatt ggcatcgacg ccgccggtca gcaccttgcc cgaggacggc
     7801 actacggtgt tgtaggcacg cgccaggcgg gtgatggagt cgagcaggat cacgacgtcc
     7861 ttcttgtgct cgaccaggcg cttggccttt tcgatgacca tctctgcaac ctgcacgtgg
     7921 cgggccgcag gctcatcgaa cgtgctggag acgacctcgc ccttcacggt gcgctgcatc
     7981 tcggtgactt cctcgggccg ctcatcgatg agcagcacga tcagatagac ctcgggatgg
     8041 ttggaagtga tcgcggtggc gatgttctgc agcagcatcg tcttgccggc tttcggcggg
     8101 gagacgatca gcccgcgctg tcccttgccg atgggcgcca ccagatcgat ggtgcgggcc
     8161 gtcaggtctt ccgtcgagcc gttgccgcgt tcgagcttca ggcgcttggt cggatgaaag
     8221 ggggtcaggt tctcgaacag gaccttgttg cgcgagctgt cgggagagtc gaagttgatc
     8281 tcaccgacct tgagcagcgc gaagtaacgc tcgccgtcct tcggcggacg gatgagcccg
     8341 gagatggtgt caccggtgcg cagattgaag cggcggatct ggctcgggct gacgtagatg
     8401 tcatcgggac cggccaggta ggagccatcc gccgagcgca ggaagccgaa gccgtcctgc
     8461 aggatctcca gcacgccatc gccgtagatg ttctcgccgt tgcgggcgtg cgccttgagg
     8521 atcgagaaaa tgatgtcctg cttgcgcgag cgggccaggt tctccaggcc catcgactcg
     8581 gccagcttga ccagctcgtg aatcggccgg gacttcagct cggtcagatt catcgaagcg
     8641 cgtgactgcg tgagctcagc cgggctgatg atttcggcgt catcgacgtc gaacatctcc
     8701 ggctcgtgca tcatctcatc gggacgaccc ccgccgttgc tgttgccgcg gttgccgtca
     8761 cggttgccgt gccgcggtcc tttgttgggc cggttgcggc cctgattgcc gaggtagcct
     8821 ctcattttct tgccagcgcg gcggccgcgt ccaggttgct atccaggaat gccgcgagtt
     8881 gggatttgga gaccgccccg accttctgcg cttccacggt gccgttcttg aacaggatca
     8941 gcgtcgggat gccgcggatg ccgaacttgg gcggagtctg cgggttctcg tcgatattga
     9001 gcttggcgat cttgatgcgg tcctggtatt cgttcgcgat ctcatcgagg atgggagcga
     9061 tcatcttgca ggggccgcac cactcggccc agaagtccag gagtacgggt ttgtcggact
     9121 tgagcacatc ctgggagaat gtggcatcgg tggtgtgcac gatgtgcggg ctactcacgc
     9181 ggttcaacct ccgtcgactg cagagtgtgg gaatggtttg ttcggataag aggacttaag
     9241 gatcgccggg tcggggcggg ccagcgaaag ccatgccaga atcgcccgtc aaacggttac
     9301 actaactcgt gaagtgagta tggaacttga gctttcaggg attcgccttt cggctggcag
     9361 gtgcggcccc ttgcttgttg cactggcgcc gtcttgtcca ctttgtagcg cttccgggcc
     9421 gcttttgcaa ggcccgggcg gcctgcccac taccgtatag aacgatttga tgactgatcc
     9481 acattccggt tcccctgagt tcgcatccct gggtctcgcg ccgcccgtga tggagggtat
     9541 ccgggcagca ggcttcgtca cgtgcacccc gatccaggcg cagacgctgc cgattgccct
     9601 gtccggacag gatgtggccg gacaggcaca gacgggcacg ggcaaaacag ccgccttcct
     9661 ggtcgcgctt tatcaggccc tgctgacacg cccacgccct gctggacacg gccgtgcctc
     9721 catacgcgcc ctgatcgtgg cgccgacgcg cgagctggcc gtgcagatcc atcatgatgc
     9781 ggaaaccctc ggccgcttca ccggattgcg tcaggctgtc gtattcggcg gcgtcgacta
     9841 cgacaagcag cgtcgccaac tcgaggaggg cgtggatgtg ctgatcggca cgccgggccg
     9901 actcatcgac tacttcaagc agcgcgtgtt cgagctgcgg cacgcgcagg tcgtggttct
     9961 cgacgaagcc gatcggatgt tcgacctggg cttcatcgcc gacatccgct acctgctgcg
    10021 gcgcctgccg catccggaac accggcaatc gatgctgttt tcggcaacgc tgtcgcatcg
    10081 ggtgctggag ctggcctacg agcacatgaa caaccccgag ctcgtgcgta tcgagcccga
    10141 caagatgacc gtcgaccagg tccgccagct gatgtactac ccggccatgg atgagaagtt
    10201 gccgctgctg gtgggcctgc tgcgtcaata cgaggcacgc cggaccatga tcttcgtgaa
    10261 caccaaacgt gccgccgaac gcctcgaaag cacgttgcgc gccaacggat ttcacgctca
    10321 ggcgctttcg ggagatgtcc cccagaacaa gcgcctgaag ttcctgcgcg acttccacaa
    10381 cggcgatctc gccgtgctca tcgcaaccga cgtggcctca cgcgggctgc acattccgga
    10441 agtcagccac gtcttcaatt tcgacctgcc gcaggattcg gccgactacg tccaccgcat
    10501 cggccgcacg gcgcgcgccg gcgcggaggg cgatgccatc agcttcgcct gcgaggagta
    10561 cgcggtctcc cttcccgaca tcgaggagta catcggccac aagattccat ccatgccgat
    10621 cgcaccggag ctgctggcaa cagcagtggc cgccgcgcca gccgcagagc ggcatcggcc
    10681 gcggcacgtc cgccccggcg ggcactcctc cggccgtcgc ccaccacccc gccgccacgg
    10741 caagccgccg cgtgggcggg gccgctgatg gggctggggt tggaaccggc tgagctgacc
    10801 ttcatggggg tgccacgaca tgatgtcaag gccacccgtg gcgggaccag cggcgtttcg
    10861 ctaagctagc gcccggcggc cgcttcagcg agcccccatc catgggagac cgcgggctgc
    10921 accagccatg gcggaaatcg aagacaacgt tgaccgggag ctgttccgca acatcgagaa
    10981 gttgcgtcag ctgcgtatcg agcatcgtga cctggatgag gtgatcgagc ggctcacgat
    11041 ggacctgcat accaacgagt tgcaattgaa acggctcaag aaacgcaagc tgatgctcaa
    11101 ggaccagatc acgcgcatgg agagccagct cattcccgac ctgaacgcgt gaaccacgta
    11161 gacagcgcag atggcgcctt gatgggcgcc tttctacgcc tgacctcccc gcagatcctg
    11221 actgaggtca ttgcgattgc ctgcgcgggg ctgatcgccc tcgccggggc gcgaatcgtg
    11281 cgcgcctggc acaaacgcca cacgaccgac ggtcgcggtg agggctggca ggccgatgtg
    11341 caggagggga cgatcatcgt cgccccctac ctggtggcgc tggttgttct gctgctggtg
    11401 cgctcgatcc taagcttctt gcacgcccat acctcagcga tcgacaccgc cctgcagctg
    11461 accaccgccc tcgtactggt acggctgggc gtgtacctcc ttcgcgtcat gatgggaccg
    11521 gactcgtgga tccgtacgtg ggaaaaccgg atcacgctgg tggtctggct cacgatgtcc
    11581 ttctcgctcg tcggttggct cgatgcggtc gagaacaccc tcaaccacat caacctgatc
    11641 ccgggcaaga cacagttcag cctgtgggcc ctgctgaagg gactggtggt ggtgtcggcg
    11701 ttcgtcgtcg tcaccagcct gatcgcgcgg gctttcgaga agcgcgtcat gcgcctgccg
    11761 cagctggccg tctcgacccg catcggcatc tccaagttcg cctacttcat cctggtcggc
    11821 atcggggtga tgctcggcat caacgaggcc ggcgtcgatg tcacgaccct caacgtcctc
    11881 accggcgcaa tcggcctggg gctgggtttc ggcctgcagt cgatcgcgag caacttcgtc
    11941 agcggtttcg tgctgctgat ggacaaatcc atcaagcccg gagatgtcat cagcttcacc
    12001 gggcacaccg gaaccagcac cgagaatttc ggctgggtcc aggagctgcg cggccgctat
    12061 gtggtggtcc gcgaccgcga tggggtcgag accctggtcc cgaaccagaa cctcatcacc
    12121 aactcggtca tcaactggag ctattccgat cagcgcgtgc gcattcgtct gccggtcatg
    12181 atcagctacg aggatgaccc ggagttcgcc ctcaagctgc tgctggaggc cgtcgcgcac
    12241 cactcgcgca tcctgcgcga acctccgccg gtcacgcggc ttctggcctt cgaggactac
    12301 ggaatgcgca tcgaggtgcg cttctggatc gccgatccga tgaatggtgt caacaatgtc
    12361 cgatcggaca taaaccgatt gatctggaag ctcttccggg aacacggagt gaagatgccc
    12421 gtggcgcaaa gggagttccg catgctggac cccgtccgct ccacgcagat tcccagccga
    12481 cccttgccga ctccacccga agagtccgtc ggggattgac ccggcggcgc ggaacgagca
    12541 tgcccctgga aatcgccccg gacgtggtga ttgcggactc cgagctcgag gtctccttcg
    12601 tacgctcgtc tggtccaggc gggcaaaacg tgaacaaggt cgcctcggcc gtgcagctgc
    12661 gcttcgatct cgccgcaagc agcgcactca ggccggaagt caaagcccga ctgcgtgcgc
    12721 ttgccggccg ccgcctcacc gacgagggtg cgctgttgat catcgcgaga aatcaccgca
    12781 cgcaggagca gaatcggcgc gaggcgctgg agcgactgaa ggatctggtg gtgcgcgcgc
    12841 tggtcgctcc caagccccgc cggcccacca agcctacgcg ggcctcacgt caacggcgcc
    12901 tcgacagcaa ggcacaccgg cgcaccacca agggtctgcg cgccaaggtt cattgggaga
    12961 gttgatcgca tggatctgat gcccgccttg atggccttcg tgcatcacct ggccgccttc
    13021 accctcgtcg gcgccctggc cgccgaagtc gtactgttca agccgcctct gacggttccc
    13081 caggcgcgca gagtgcagcg agccgatctg gtgttcggcg cggcggccac gctggtgctc
    13141 gtcgtcggac tgctgcgcgt gttcttcttc gaaaaaggag ccggctatta cttcagcaac
    13201 gtcttcttcc tgaccaagct gtcgctgttc atcctggctg gattgatttc catctacccc
    13261 accgtcctgt ttctgtcctg gagcaagact ctgcgccagg ggaacacgcc cacgatcgcg
    13321 ccggagcagg tgcgccgggc ccggatgtgc atgatgctcg agctcaccgc gattctcggc
    13381 atcctcatct gcgctccgct catggcacgc ggattcggag tcatgggtta atcctggcgt
    13441 gcggcctcgc gctgcatgag ctgccacagc tcggccacgt gatgctgctg cgtggcttcg
    13501 ttgccgaagg aaactcggac catccagcgt ccatcgagga ttgccggcgt gagataggcc
    13561 tgacccgact cgttgatgcg gcgcacccaa ccgagcgtgt gggcatcgag cgcctcgcct
    13621 gcgaggccca cgggctcgtg gcgaacacag acggtctgca gagggaccgg cgccagccgc
    13681 ttccaacctg gcgtgccatc cacccgtgcg gcaaaccaac gtgcgtaatc gatgtctcgg
    13741 cgcaaccgcg cctgcaggcc actcacgccc tgctcgcgga tcaggaacca tagcttcagg
    13801 gcgcgaaagc gacgcccgag cggaatgccc cagtcacgga aattcttcac ctgcgcatca
    13861 tcggccgtgc gcaggtagct cgggttggta gacatgaccc gcacgagatg ttggggatcg
    13921 cgcacgaagt aggtggagca atcgaaggag gccccgagcc acttgtgcgg gttgatcacg
    13981 agggagtcgg cgccttcgat gccttcccac aaggtgcggc actcgggcag gatcatggca
    14041 gacccggcga gggcggcatc gacatgcatc cacagagcgt gtgtctgcgc cactttggcg
    14101 atcccggcca gcggatcgat tgccgtggtc gctgtggttc cggtcgtggc caccaccgcg
    14161 cagggtttca acccgttcga caggtcggtc ctgacggcct cctcgagtgc ttcgggtcgc
    14221 atagcgaaat gctcgtccac gggcacggcg tgcaggttga cgcggccaaa gccggccatg
    14281 taagccgcct tgtccaccga gctgtggctc tggctggaag cgtagacgac cagtgccttg
    14341 gcctcgccct gcagcccccc gcggccggcg ccatagtcgc tcgcccgctc ccgagcgcac
    14401 agcaggccga cgagcgtccc gctcgaggcc gtgtcctgga tcacaccgct ccagctctcc
    14461 gacaggccga gcatctgacg cagccagtcg atggcgactt cctcgagctc ggtgagcgca
    14521 ggactcgcct gccagttgag gccaagctga cccagaccgg tgctcacctg atctgcgagc
    14581 accgccgcca gcgagctgtt ggagggaaag taggcgaaaa aacgcggatc cagccagtgc
    14641 gtgcaggcag gcaggatcag ctcatcgagg tcatgcagca gcacatcact gggctgcggc
    14701 tcgagcggtg gagatttcgg caacgcacgt ttcaagctgc ccgggctcat gcggggcgtg
    14761 accggaaact cgccacggcc ggcgcgctcg cgatagtccg cgatccaatc gatcagctca
    14821 tggcccacac gccgaaactc ctcgatattc atcggttgac ctctcctcgc cggtgcttgc
    14881 tcagaagggc acgcgcgtcc cgatgccatc gcgcaccgct cgctcgtaaa tcgcattcgc
    14941 cgcagccaga tcctgggcgg cgatgccgtg cgacttgtac acggtgatct cccggtcacc
    15001 ggtgcgcccc ggcacccgcc cgctcagaac ctcgccgatc tccccgagta catgcccctc
    15061 ggtaacgagt cccgcctcct gcgcgtgccg cagctcagcc gcctcagcgg ccgcggacgt
    15121 tttggagtct acgaagaagc gcgagcgcgc cacgacctca tcgtcggctt ctcgcgccgg
    15181 tagccggctc gagccgacaa gattcacgtg ggtgccgcgg gcaagcgcgg ctccacgcag
    15241 gatgggctcg cgcgcggcag tgacagtgca gacgatgtca gcgccatcca ccgcttcatc
    15301 ggccgtctca cgggcctcga tcggcaagga aaacctcgcg ctctgctcat ccgcaaactt
    15361 ccgcgccttg gctgaggtgc gcccccagac gctcacgctg cgcagcggcc gcaccagtgc
    15421 aagcgcctcc agatgagtga cggcctgctc gcccgtgccg aggatcgcca gcctgtcggc
    15481 atcctcacga gccagagctc tagtggctac agcggtggcg gcggccgttc gcagcgcggt
    15541 gatcattccg gcatccatca ccgccagcgg atgccccatt tccgcatcaa acaggacgac
    15601 caccccgacg tgcgagggca gaccccgctg ggcgttctcc ggataaacgg tcaggatctt
    15661 ggcaccgagc ccgcgcggct cgccgagata gccggacatc actccaaaga accggcgccc
    15721 ctggggcagc gcgaagacgg tgcgcagcgg gatgttggca tccccgcgcg agaaggcgcg
    15781 catggccaac tcgacggcgg tgatgcaatc ggccatcggt aatgcgcgtc ggacatcctc
    15841 cctgccgacg acgagaagac cgggaccgct cgcgggagtg tggctcatgg gattgctccg
    15901 ggactgtgca ggctgatcga ggcgtgctct caggtgtcga gtccggtcac ctgcagaatg
    15961 ctgtgtcgga tctgctcgct caggacgaat tgcgcatcgg cagcgacccg cagcaggacc
    16021 tcgtcgaact cgagcttgcc gcttgccgac aggcgaatga ccccgcgagc gcgcagcagg
    16081 tcgatgaagt tctcgaacat ggtgcgatcg aagaactccg gcgaattcag tccgtacagc
    16141 agcgtcatgc gctgcgcagt gagctggcag cgctcctcga ggactcgctg gctgatcgtg
    16201 ccgctgcccg ccttcaccag ctgtgccacg gccagatagt agcgctcgat ggtctggatc
    16261 gtcgcctgcg cgagcagtga aagctgcatg gccttggtcg agtccggcgg cggtcgccgc
    16321 cactcggtgc ggtcgggatc gccttcgacc aggccatgca gctcgaggca agcgagcact
    16381 tcgttcacca catccggcaa ctcacgctcg ttccaacgca gaaacagctc ggccgcaatg
    16441 taggggtaga tgcgccaggc cagacgctgg atgtcctcgg tccgcaccct ggcattgctg
    16501 acgaacgcgc aggcgaccag cgagggcatc gcgaacagat gcaggatgtt gttgcggaaa
    16561 taggtggcga ggaccgcgct ctcatcgctc atgcggatga tgtcgcccag cggatgaggt
    16621 tcccgcgaga caaccttcat ggattctccg taggcgagaa tctgcgccgg ggtgtgtgag
    16681 gtcacggtca cccggtcgct gtacgcgaag ccgcgcaaca ggtcgcgata cagatcgagt
    16741 tgacgcagca gctcgggttt cggcagccgc tggcgcggca tggccagcag cgtgatggcc
    16801 agcaggttga tcggagtcac cgcagctgcc gcattgatgt ggcgcatgat cgtgccggcg
    16861 agatcatcga ctgcggcccc aatccagggc acacgcgtct cgtcctccac cggacggccc
    16921 cgccagtccg gatcgtagcg ctcgagcagc tcggccagat ggatgggttc gccgaggttc
    16981 acgtgcacct ggccgaaacg ctcgcgcatg cagcgcaagg tgcggatcag cccgaagacg
    17041 gtttccttct ccttgggctt gccggacagc tccccgatat aggtcgagcc ctcgacgatg
    17101 cgctcgtagc cgaagtagac cgggatgaag acgaccggac gtccggggtc gcgcagaaag
    17161 ctgcgcaccg tcatcgagag catgccggtc ttgggctgca gcagccgccc ggtgcggctg
    17221 cggccgccct cgatgaagta ctcgatcgag tggccgcggg ccatgatggc ggccaggtac
    17281 ttcatgaaca ccacggtgta cagggcgttg ccacggaagc tgcgccggat gaagaacgcg
    17341 ccgcccttgc gcagaaaccg gcccacgatc ggcaggttca ggttgatccc ggccgcaatg
    17401 tgcgggatgg caaagccatg cacgtatatc gcatacgaca gcagcagata atccatgtgg
    17461 ctgcggtggc acgggacgta gatgatctcg ttcccctcgg ccacctcctc gagcgtctcc
    17521 acgtgaccga acaccacccc gtcatagagc cggttccaca ggcgtgacag ggccgcctcc
    17581 atgaaacgga tgaacgcatg cgagtagttc gcagcgatct ctcccgcata ctgtcgcgcc
    17641 ttcagcatgg cctgacgccg gtcgaggcct ttctcgcgca tctcctgcgc caccgccgct
    17701 ctgacggcgc gcgtgcgcag cactcggttg aggatggtgc ggcggtgcga gaggtcgggg
    17761 ccgatccggg cggagcgctg tcttgcgtac aacacgcgga gcgctcgcgc gacacgccgc
    17821 ccgcgtatcg ctgtgccggc ctcatcgccc agcagacttc gcagtgagac gggctcgtcg
    17881 aactcgatta gcgtgttgcg cccgttgaac agcacttgca tcagcttgcg catgcggctg
    17941 gcgagtgccc actcctcgac gagcagcagt cggaaccagg aggcttcctt ctgcggcgcg
    18001 cggccccagt acaccgacac cggaacgaga tcgacatcga ggtgcggatc gtgtgcgagc
    18061 gcagcgatca tctgctgcag ctgcggaggc gggcgtctgt ctatgcgctc gtcccagaag
    18121 ccgcgcggcc tgctgaggta gaagaacgag cgcagatccc tggtagcggg catgagccgc
    18181 ttgcgcggtc tcgcgagctt caggcggacg caggcgttct gcagaaccgc gaggtccgcg
    18241 gcactgcgcc gctcgagcgc atagcagacc ggccggtcgc gctcgcgcag gtgggccgcc
    18301 gcctcatccg gcctgatctt gaatcgcacc cagagcgcca gcagccgccg caacagccag
    18361 ttcattcact gaaacgcgat ggccgggagg tcgagcgcga tgaggaagta ctcgaacaga
    18421 tactgaatga cgcggatctg gttgtgcagc cggtgatcgc tgtcgagcac gtgcagcgca
    18481 g
//
LOCUS       DASRHN010000244         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_412079, whole genome shotgun sequence.
ACCESSION   DASRHN010000244 DASRHN010000000
VERSION     DASRHN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412079"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2510)
                     /locus_tag="VFB37_09825"
     CDS             complement(<1..2510)
                     /locus_tag="VFB37_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HZO22787.1"
                     /translation="MHDQSAKIQVPRPYPGRPGKQGLYDPQYEHEACGVGFVVDVKGR
                     KSHRILQQAIQVLRNLDHRGACGCEANTGDGAGVLLQMPHAFFQQIARKDRLTLPGPG
                     EYGSGLVFLPRNPTKRRRLVEVFEHIIQSEGQTVLGWRTVPTNNSMLGETAKASEPFI
                     RQIFIGRNSAIQDDMEFERKLYVIRKRAYSEIRTSTIDGAEYWYLASLSHKTIVYKGM
                     LLTEQLAQYFTDLQQPAVETALALVHSRFSTNTFPSWDRAHPYRYLAHNGEINTLRGN
                     YNWMKAREALFDSDLFGADTHKIRPIINPNGSDSAMFDNTLELLVLAGRSLPHAMMMM
                     IPEPWSNHESMDDGKRAFYQYHSSLMEPWDGPASIAFTDGKMIGAVLDRNGLRPSRYY
                     VTKNDLVVMASEAGVLDLPADEIVQKGRLQPGRMFLVDTEQGRIIDDEEIKRTVATEH
                     PYRRWLDQHLVHLRDLPSAPEVPASDPDSLLQRQIAFGYTFEDQRVLLEPMARNGVEA
                     VGSMGNDTPLAALSSRPRLLYDYFKQLFAQVTNPPIDCIREELITASEVWLGSEGNLL
                     RPQPGDCRRLELQGPILTNEEFAKVRRMNVPGLKVGVLSILFRATRGEKGLTKSMEEL
                     CLAARRLIEDEEVNVLILSDRGVKREFAAVPALLAVSGLHHYLIREGLRMRVSLALET
                     GEAREVQHFALLIGYGCSVINPYLAFETIESMIHEGMVPNVDSKLACKNYVKAATKGV
                     VKVMSKMGISAIQSYHGAQVFEAVGLRQDVIDQYFTWTASRIGGVGMDVIAQEVLLRH
                     RAAFPAREANGHALQTGGLYQWRADGEHHLFNPESIHRL"
BASE COUNT          434 a          808 c          811 g          483 t
ORIGIN      
        1 aggcggtgaa tggattccgg gttgaagagg tggtgctcgc catcggcgcg ccactggtag
       61 agccccccgg tctgcagtgc atgcccgttg gcctcgcgcg ccgggaatgc ggcgcggtga
      121 cgcagcaaga cttcctgggc gatgacatcc atgccgacgc cgccgatgcg cgaggcggtc
      181 cacgtaaagt actggtcaat gacgtcttgg cgcaggccca cggcctcgaa cacctgtgcg
      241 ccgtgatagc tctggatcgc cgagatgccc atcttggaca tcactttgac cacgcccttc
      301 gtcgccgcct tcacgtagtt cttgcaggcg agcttggagt cgacattcgg gaccatcccc
      361 tcgtggatca tgctctcgat ggtctcgaag gccaggtacg gattgatcac cgagcagcca
      421 tagccgatga gcagagcgaa gtgctggact tcccgcgcct cgcctgtctc caacgcaagg
      481 ctgacacgca tgcgcagccc ttcgcgaatc aggtagtggt gcaagccgct gaccgcaagc
      541 agcgccggta cggccgcgaa ctcccgcttc acaccgcgat cgctgaggat gaggacgttg
      601 acttcctcgt cctcgatcag ccggcgcgcg gccagacaca gctcctccat cgacttggtc
      661 agtcccttct cgccccgtgt ggcgcggaag agaatcgaga gcacgccgac cttgagtccc
      721 ggcacattca tgcgccggac cttggcgaat tcctcgttcg tcagaatcgg tccctgcagc
      781 tccaggcgcc gacagtcgcc gggctgcggg cgcagcaggt tgccctccga gccgagccac
      841 acctcggagg ccgtgatcaa ctcctcgcgg atgcagtcga tcggcgggtt cgtgacctgc
      901 gcgaacaatt gcttgaagta gtcgtacagc agtctgggcc ggctcgacaa tgcagccagc
      961 ggagtgtcgt tacccatcga gccaacggct tccacgccgt tgcgcgccat gggctcgagc
     1021 agcacgcgct gatcctcgaa cgtgtagccg aaggcgattt gccgctgcag gagcgagtcg
     1081 ggatcggacg ccggcacttc aggagccgag ggcaggtccc gcaggtgcac caggtgctgg
     1141 tccagccagc gccggtaggg gtgctcggtc gcgacggtgc gcttgatttc ctcgtcgtcg
     1201 atgatgcggc cctgctcggt gtcgacgaga aacatgcggc ccggctgcag ccgtcccttc
     1261 tggacgatct catcggcagg caggtcgagg acaccggctt cggaagccat cacgaccagg
     1321 tcgttcttcg tcacgtagta gcgggaaggc cgcagtccgt tgcgatccaa caccgccccg
     1381 atcatcttgc catcggtgaa agcgatcgac gccggcccat cccacggttc catgaggctg
     1441 gaatggtact gatagaaggc gcgcttcccg tcgtccatgc tctcgtggtt ggaccacggc
     1501 tccgggatca tcatcatcat cgcgtgtggc agcgaccgcc ccgcgagcac cagcagctcc
     1561 agcgtgttgt cgaacatcgc ggagtcgctg ccgttggggt tgatgatggg ccggatctta
     1621 tgcgtatcgg ccccgaacag atcggagtcg aacagcgcct cgcgcgcctt catccagttg
     1681 tagttgccgc gcagcgtgtt gatctcgccg ttgtgcgcga ggtacctgta cggatgtgcg
     1741 cgatcccagc tcgggaaggt gttggtgctg aagcgggaat gcaccagtgc gagcgccgtc
     1801 tccacggccg gctgctgcag atcggtgaag tattgcgcga gctgctcggt caggagcatt
     1861 cccttgtaga cgatcgtttt gtgggagagg ctggccagat accagtactc ggcgccgtca
     1921 atcgtcgagg tgcggatctc actgtaggcg cgcttgcgga tcacatagag cttgcgctcg
     1981 aactccatgt cgtcctggat cgcggagttg cgtccgatga agatctgacg gatgaagggc
     2041 tcgctggcct ttgccgtctc cccaagcatg gagttgttgg tcggcaccgt gcgccagccg
     2101 agcacggtct gtccctcgga ctggatgatg tgctcgaaca cctcgaccag gcggcgccgc
     2161 ttcgtcgggt tgcgcggcag aaacaccagg ccgctgccgt actctcccgg tccgggcagg
     2221 gtcagccggt ctttcctggc gatctgctga aagaaggcat gcggcatctg cagcaaaacg
     2281 ccggccccgt cacccgtatt ggcctcgcag ccgcacgcgc cgcggtgatc caggttgcgc
     2341 aggacctgaa tggcctgctg caggatccgg tgggatttac ggcctttgac gtccacgacg
     2401 aagccaactc cgcaggcttc gtgctcgtac tgcgggtcgt acagaccctg cttcccgggc
     2461 cgccccgggt aggggcgcgg gacctgaatt ttcgcgctct gatcatgcat agtcggccac
     2521 caaatccgga caccca
//
LOCUS       DASRHN010000245         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1898016, whole genome shotgun sequence.
ACCESSION   DASRHN010000245 DASRHN010000000
VERSION     DASRHN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898016"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFB37_09830"
     CDS             <1..448
                     /locus_tag="VFB37_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZO22788.1"
                     /translation="MHLLAGGYSGWREFLLAQADATMSFLQRTCDSVDRCTWGKRNRI
                     RIQHPLSRALPFLGWLLDMPTVELPGDNDMPRVQGNTFGASERFGVSPGHEAQGYFHM
                     PGGQSGHPLSPYYRAGFMAWARGEPLPFLPGASEHRVTLRPLNSQR"
     gene            490..1689
                     /locus_tag="VFB37_09835"
     CDS             490..1689
                     /locus_tag="VFB37_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZO22789.1"
                     /translation="MNSPFETSYRSAGRPTVGLVLTGGGARSAYQVGVLLALAELMPR
                     ARNPFQVIVGTSAGAISAGVLASEAYYWRRAVAGLERVWANFHSAQVFHVDPAHMLRA
                     GLHWVLALVSGGLILSPPKSMLDNSPLRQLLGVHVDCAGIKRSIARGHLRAIALCSTS
                     YGTGQSVAFYDGIEPIPEWARVQRVGRRTDLTLDHLMASSAIPLLFPAMKLGEEYFGD
                     GAMRQLNPLSPAIHLGADRLLIIGVRARRAAGVVVNRRETSMPTPGEIFGYMLDTLFT
                     DQVYGDLEQVEKINQLVTAAPEAARGLRYVETLMLAPSVDPREIAARHMAEMPPGLRT
                     LLRVIGGRDSGLQLPSYLMFEAGYTRELIQLGYRDAMEARSALLAFMSGEKLPPVMTA
                     AGVAQAT"
     gene            complement(1690..2088)
                     /locus_tag="VFB37_09840"
     CDS             complement(1690..2088)
                     /locus_tag="VFB37_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22790.1"
                     /translation="MTEDLPRAETPLKVLVVDDQPDSADAVAFMLSLMGHDVHTAYGP
                     NEALELFRQVQPAVVFMDISMPELSGLEAAARIRSMPQGTSTTIIALTGLGHEADRRR
                     SREASIDYHVVKPATPAVLHRLLSAIYPGA"
BASE COUNT          341 a          722 c          732 g          415 t
ORIGIN      
        1 catgcatctg ctcgccgggg gctattccgg gtggcgggag ttcctcctcg cccaggccga
       61 tgccacgatg agcttcctgc agcgcacttg cgacagtgtg gatcgttgca cctggggcaa
      121 gcgcaaccgg atccgcattc agcatccgct gtcgcgcgcc ctgccgttcc tcggctggct
      181 gctggacatg ccgaccgtgg agctgccagg cgataacgac atgccgcgcg tgcagggcaa
      241 taccttcggg gcctccgaac gctttggcgt ctcccccggc cacgaggcgc aaggctactt
      301 ccacatgccc ggcggacaga gcgggcatcc tctctccccc tactacaggg cgggcttcat
      361 ggcgtgggca cggggtgagc cgttgccatt tctgccgggt gcgtccgagc acagggtcac
      421 cctgcgtccg ctaaactcgc agcggtgaag ctgacggcgg agcatgacgg ccgtcacagt
      481 cccgatgcga tgaacagtcc tttcgaaaca tcctatcgct ccgcggggcg gcccaccgtc
      541 gggctggtgt tgaccggcgg cggcgcccgg tccgcgtatc aggtcggcgt gcttctggcg
      601 ctcgccgagt tgatgccgcg cgcgcgcaat cctttccagg tgatcgtcgg gacctccgcc
      661 ggagcgatct ccgccggcgt gttggcatcg gaggcctact actggaggcg cgccgtcgcg
      721 gggctggagc gcgtgtgggc gaacttccac tccgcccagg tattccatgt ggacccggct
      781 cacatgctgc gcgccggcct gcactgggtg ctggcactgg tgtcgggcgg cctgattctc
      841 tcgccgccga agtcgatgct cgacaactct ccgttgcgcc agctgctcgg agtgcacgtg
      901 gactgcgccg gcatcaagcg cagcatcgcc cgcggccacc tgcgagcgat tgcgctttgc
      961 tccaccagct atgggaccgg tcagtcggtt gcgttctatg acggtatcga gcccattccc
     1021 gaatgggcgc gcgtgcaacg ggtcggtcgg cgcaccgacc tgacgctcga tcacctgatg
     1081 gcgagctctg ccatcccgtt gctgtttccg gcgatgaagc tcggcgagga gtacttcggg
     1141 gacggggcca tgcggcagtt gaatccgctc agccccgcca tccacctcgg cgccgaccgg
     1201 cttttgatca tcggtgtgcg cgcccgccgt gccgcgggtg tcgtcgtgaa ccggcgcgag
     1261 acttcgatgc ccacacccgg cgagattttt ggttacatgt tggatacgtt gttcacggat
     1321 caggtctacg gcgatctcga gcaggtcgag aagatcaacc aactcgtcac cgccgccccc
     1381 gaagccgcac gcggcttgcg gtatgtcgag acgctcatgc tcgcgcccag cgtcgatcct
     1441 cgcgagatcg ccgcacgcca catggccgag atgccgccgg gcctgagaac gttgttgcgg
     1501 gtgattggcg gccgcgactc gggcctgcag ctacccagtt atctgatgtt tgaagccggt
     1561 tacacccgag agctgatcca gttgggctac cgcgatgcga tggaagcgcg gtcggctctg
     1621 ttggctttca tgagcggcga gaagctgccg ccggtgatga cggcggccgg ggttgcgcag
     1681 gcaacgtaac taggcgcccg ggtagatcgc gctgagcagg cggtgcagta ccgctggggt
     1741 cgcaggcttc accacgtgat agtcgatgct cgcctcccgt gaccgcctgc gatcggcctc
     1801 atggcccagg cccgtgagcg cgatgatggt cgtcgaggtc ccttggggca tcgaacggat
     1861 gcgcgcagcg gcctccagcc cgctcaattc cggcatgctg atatccatga acaccacggc
     1921 gggctggacc tgccgaaaca gctccaaagc ctcattgggg ccgtacgccg tgtgcacgtc
     1981 gtgacccatg agggagagca tgaaggccac ggcgtcggcg gagtccggct ggtcatccac
     2041 gaccagcacc ttgagcggag tctcggcacg aggcagatcc tcagtcatcg ttgacccctg
     2101 ctgttcgtgt tcgagcatct gtctttccgg tgtggtcatg cgacgactgc gacgtagcaa
     2161 gtagtgtgcg cgctaggcgg gcgcagtgtc gccgtactgc aacataaccc
//
LOCUS       DASRHN010000246         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1403303, whole genome shotgun sequence.
ACCESSION   DASRHN010000246 DASRHN010000000
VERSION     DASRHN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403303"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1096
                     /gene="era"
                     /locus_tag="VFB37_09845"
     CDS             197..1096
                     /gene="era"
                     /locus_tag="VFB37_09845"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZO22791.1"
                     /translation="MSDTPSDFRSGFAALVGRPNVGKSTLLNALVGQKVSIVTPRPQT
                     TRHRIVGISRLPAAEIAFVDTPGLHHQANRALNKAMNRTAASALEGADLVVQVVEATR
                     WNTEDDLALERIKSSGRPAMLAVNKIDRVHPRELLLPYLASVAKKHEFLEVVPISALK
                     ADGIADLQRTIAKHLPQAPPMFPDGELTDRGREFRIAEMIREKLTLELNQEVPYGIAV
                     EVERMVEEDGQLVVDAAIWVDREGQKPIVIGAKGERLKRVGRSARLALNELLGQRLHL
                     TLWVKVRENWADNARALNELGLE"
     gene            1093..1824
                     /gene="recO"
                     /locus_tag="VFB37_09850"
     CDS             1093..1824
                     /gene="recO"
                     /locus_tag="VFB37_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZO22792.1"
                     /translation="MTRSARRIQLTPGYILHHRPYRDTSRILEVLTREHGRLSLFARG
                     VRGPKAKLASVLQPFQMLLFSWNGRGEAAQLTGAESAHAGPPLPSSCLMAAFYLNELL
                     LKLTTRHDPLPLLFDTYHDTIEGLRQGESLEPRLRIFEKRLLEALGYGLELSAEAQTG
                     KRVEPNAYYHFRPEQGLVATVAQAAGALAGTSLIGLASEHLAGERELEDAKRLLQAAL
                     AQCLEGRPLATRMVARAVARRESLT"
     gene            1852..2586
                     /gene="pdxJ"
                     /locus_tag="VFB37_09855"
     CDS             1852..2586
                     /gene="pdxJ"
                     /locus_tag="VFB37_09855"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZO22793.1"
                     /translation="MSYKTIFLGVNIDHVATLRQARRGRYPDPVHAALTAEMAGADSI
                     TLHLREDRRHVQIHDVRAVRDLQKTHMNLEMAVTDEMLDIARVVRPSDCCFVPERRQE
                     ITTEGGLDVRSQVQRIKEATTMLTGQGIRVALFIDPDPPQIEAAAQTGAPAVELHTGA
                     YADSTGSRQAAELERLRAGAQLAASLGIEVHAGHGLHYHNVQPVAAIKEIVELNIGHA
                     IISRAVFDGLAVAVREMKALMVAARG"
     gene            2583..2969
                     /gene="acpS"
                     /locus_tag="VFB37_09860"
     CDS             2583..2969
                     /gene="acpS"
                     /locus_tag="VFB37_09860"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZO22794.1"
                     /translation="MIFGIGVDVLEAKRIDETLERFGTRFVEHLLMPAEHVQLAKTQR
                     PARFLAMRFAAKEAIVKAMGTGFAHGIWIRDVGVVQNSWGKPEVVFSERGERVRQALG
                     IGEGHVTLTDEAGLVVAVAVLMKATQ"
     gene            2980..>3674
                     /locus_tag="VFB37_09865"
     CDS             2980..>3674
                     /locus_tag="VFB37_09865"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZO22795.1"
                     /translation="MNCLVFDIETIPDVALGRRLYNLDGLADEQVAKAMFALRRQSTG
                     GDFLPNEQQRVVAISCALRSRDGFKVWSLGDVSSTEGELLERFFDGIERFSPDLVSWN
                     GSGFDLPVLTLRALAAGVQAPRYWETGDGDTAFRYNNYLSRYHWRHTDLMDVLAAYQS
                     RARVSLQNAACLLGLPGKLGFSGAQVWDAWLAGNITGIRQYCETDVLNTYLIYLRFQM
                     MRGHFTRERYAEEV"
BASE COUNT          646 a         1145 c         1230 g          653 t
ORIGIN      
        1 gatcgagggc gaggctcacg cccagacctt ccatgtgacc tgcgaggtgg ccgacctcga
       61 gcgccgcagt gagggcagtg gctccagccg ccgccgggcc gagcaggaag cggcggccaa
      121 aatccttctg gagctcgcgg acgcggttta aactgccagc cgtgcgcctt ttcgcgcctc
      181 gcagacaggt tcttttttgt cggacacacc ctcggatttc cgcagcggct ttgccgctct
      241 cgttggccgc ccgaacgtcg gcaagtcgac tctgctgaac gccctcgtcg gccagaaggt
      301 gagcatcgtg acgccgcgcc cccagacgac gcggcaccgg atcgttggca tttcccgctt
      361 gcctgccgcc gagatcgctt tcgtggacac gcccgggcta caccatcagg ccaaccgggc
      421 gctaaacaag gccatgaatc gaaccgcggc gagcgccctg gagggcgccg acctggtcgt
      481 gcaggtggtc gaggcaacgc gctggaacac ggaagacgat ctcgctctgg agcggatcaa
      541 gagctccggg cgacccgcca tgctggcggt caacaagatc gaccgcgtgc atccgcgcga
      601 gttgctgttg ccgtatctcg cctcggtggc gaaaaaacat gaattcctgg aggtggtgcc
      661 catctccgcc ttgaaggcgg acggcatcgc cgacctgcag cgcaccatcg ccaaacatct
      721 tcctcaggca ccgccgatgt tcccggacgg tgagctgacc gatcgtggcc gggagttccg
      781 gatcgcggag atgatccgcg agaagttgac cttggagctc aaccaggaag tgccctatgg
      841 cattgccgtc gaggtcgagc gcatggtcga ggaagacggt cagctcgtgg tcgatgcggc
      901 gatctgggtg gatcgcgagg gtcagaagcc gattgtcatc ggcgccaaag gcgagcgact
      961 caagcgcgtc gggcgctcgg cgcggctggc tctgaacgag ttgctcggac aacgactgca
     1021 tctgacattg tgggtgaagg ttcgcgagaa ttgggcggat aacgctcgcg cgctgaacga
     1081 gctcgggctc gaatgacccg cagtgcgcga cgcatccagc tcacgccggg ctacatcctc
     1141 caccaccggc cctatcgcga cacgagtcgc atcctcgagg tcctcacgcg cgagcacggg
     1201 cggttgtcac tctttgcgcg cggggtgcga gggccgaagg cgaagctagc ctccgtcctg
     1261 cagccatttc agatgttgct gttttcctgg aacggccgcg gcgaagcggc gcaactgaca
     1321 ggtgcggaga gtgcccatgc ggggccgccg ctaccgtcct cgtgcctcat ggcggccttc
     1381 tatctgaacg agctcttgtt gaagctcacg acacgtcatg atccgctgcc gctgctgttt
     1441 gatacctatc acgacacgat cgagggactg cgccaaggag agtcgctcga gccgcggttg
     1501 cggattttcg aaaagcgcct gctggaggca ctgggctacg ggctggagtt gagcgccgag
     1561 gcgcagacgg gcaagcgcgt cgagcccaac gcgtactatc atttccgccc ggagcagggc
     1621 ttggtggcga cggtcgcgca ggcggcagga gcgctcgccg gcacgtccct catcgggttg
     1681 gcgagcgagc acctggccgg cgagcgcgag ctggaggatg cgaaacgttt gttgcaggcg
     1741 gcactggcac aatgcctcga aggccgtccg ctggccaccc gaatggtggc ccgcgccgta
     1801 gcccgccgcg aatctctcac ttgacctcgt catgagccgc tgcaagccga catgagctac
     1861 aaaaccattt tcctgggcgt caatatcgat cacgtggcta ccttgcgcca agcgcgccgt
     1921 ggccgttatc ccgatccggt gcacgcggcg ctgaccgccg agatggccgg cgcggacagc
     1981 atcactctgc atctgcgcga ggatcggcgt catgtccaga tccatgacgt gcgcgccgtg
     2041 cgcgatctac agaagactca tatgaatctc gagatggccg tgaccgacga gatgctcgat
     2101 atcgcccggg tggtgcgccc ctccgactgt tgcttcgtac ccgagcgacg ccaggagatc
     2161 accaccgaag gtgggctcga tgtcagaagt caggtacagc gtatcaagga agccaccacc
     2221 atgctgacgg ggcaggggat ccgcgtggcc ttgttcatcg atcccgatcc gccgcagatc
     2281 gaagcggccg cgcaaacggg ggccccggcg gtcgagctac acaccggcgc gtacgctgac
     2341 tccaccgggt cgcgccaggc ggccgagctc gagcgtttgc gcgcgggcgc gcagctcgcc
     2401 gccagcctcg ggatcgaagt gcacgccgga cacgggttgc actatcacaa cgtacagccg
     2461 gtggcggcga tcaaggaaat cgtggagctc aatatcggtc acgccatcat ctcgcgcgcg
     2521 gtatttgatg ggttggctgt cgcggtgcgc gagatgaagg ccctcatggt ggcggcgcgc
     2581 ggatgatttt cggaatcggt gtcgatgtgc tcgaggcgaa gcgcatcgat gagaccctgg
     2641 agcgattcgg aacgcgcttc gtggagcatt tgctcatgcc cgcggagcac gtgcagctcg
     2701 ccaagacgca gcggccggcg cgtttcctgg cgatgaggtt tgccgccaag gaagcgatcg
     2761 tcaaggccat gggcaccggc ttcgcgcacg gtatctggat ccgcgatgtg ggcgtggtgc
     2821 agaactcctg ggggaaaccg gaggtggtgt tctccgaacg cggcgagcgc gtgcggcagg
     2881 ccctcgggat cggggagggg cacgtcactc tgaccgatga ggcggggctg gtggtcgcgg
     2941 tggcggtgtt gatgaaggcg actcaatgaa ggttctggca tgaactgcct cgtattcgac
     3001 attgaaacga ttcctgacgt cgccctcggc cgccgactct acaacctgga cgggcttgcc
     3061 gatgagcagg tggcgaaagc catgttcgct ttgcgccgcc aaagcaccgg gggagacttc
     3121 ctgcccaacg agcaacagcg cgtcgtcgcg atctcctgtg cgctgcgcag ccgcgacggc
     3181 ttcaaggtct ggagcctggg ggatgtcagc tccaccgagg gcgaattgct cgagcgtttc
     3241 ttcgacggca tcgagcgctt ttcccccgac ctcgtgtcct ggaacggctc gggcttcgat
     3301 ctgccggtgc tcacccttcg ggcgctcgcg gccggggtgc aggcgccgcg ctactgggag
     3361 acgggagatg gcgacaccgc cttccgttat aacaactatc tcagccgtta tcactggcgc
     3421 cataccgatc tcatggacgt gctcgccgcg tatcagagcc gtgcccgggt gtcgctccag
     3481 aatgcggcct gtctactggg tctgccgggg aagctgggct tttccggagc gcaggtctgg
     3541 gacgcatggc tggccggcaa catcaccggg attcgccagt attgcgagac ggacgtgctc
     3601 aacacctatc tgatctacct gcgctttcag atgatgcgcg gccacttcac gcgcgagcgt
     3661 tacgccgagg aggt
//
LOCUS       DASRHN010000247        12396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1403661, whole genome shotgun sequence.
ACCESSION   DASRHN010000247 DASRHN010000000
VERSION     DASRHN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403661"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFB37_09870"
     CDS             complement(<1..973)
                     /locus_tag="VFB37_09870"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZO22796.1"
                     /translation="MDSNTTRRRQGGRGRRAAGGIAQLPWRDVTNPYPPLQVLSADQL
                     EAIHLTSLRILEELGIEVMSPRALAVLSAAGASVDADTGLVRLDRGLVQQALSSAPAS
                     FTLTPRNPAHRVVLGGNQINFGLVAGPPSVHDCERGRRPGNYSDYCDFIRLAQYFNAI
                     HLIGNQVCAPVELPANSRHLDTYRANLLYSDRVYHCTAIGAGRALDGIRMMAIARGLS
                     LEQMAQSPGVITVISVNSPRRFDAAMADGLMTMAEHAQAVVITPFTLMGAMAPVSLAA
                     ALAQQNAEALFGAVLAQLVRPGAPIVYGAFTSNVDMRSGAPAFGTPEN"
     gene            1025..2125
                     /locus_tag="VFB37_09875"
     CDS             1025..2125
                     /locus_tag="VFB37_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZO22797.1"
                     /translation="MAVIAADIAPQPPAHWRERPACAIVARDASEPGAVVVKRAGEPA
                     RIAFFLVPQFPLIAFAAGLEPLRQANRMSERRLFDWPLVSVDGKAVTASNGIPIGVHG
                     ALDTLNGIDSVVVCAGLEPTQLGRHHKAHHHLRRLARHGSRIGAISTGSFILADAGLL
                     GDHRCTVHWEYTDSLRSRHPGLKLTQDLYVIDRNVFTCSGGTSALDMMLQFVSEASGP
                     ELALAVAEQFIHPQIRHQQDQQRIGMHARYGIDSPKLVEIITLMEGAIEDPLEVTGIA
                     QHVGVSPRQVERLFREHLNTSPRAFYVRLRLTRARTLLRQTLNSVRAVAVECGFASAS
                     HFCHAYKRVFGLSPTEERQRLTGGRPRRETDG"
     gene            2118..3278
                     /locus_tag="VFB37_09880"
     CDS             2118..3278
                     /locus_tag="VFB37_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO22798.1"
                     /translation="MADPGVTPHARILILDEEQAPVAALCRMLTTEGYEVTAVSSGSA
                     ALAALRAAVADPAARFEVLIFDLKRPAKDGLTLLQAAREIDAALEAIIMTDPGHLDSA
                     MQALPGAALAHISKPFKLNTLVAGLARAQQFRRLRLENRALAEQIGQRNGQLEKANLE
                     LLRADQALEAFVHSVSHDLRQPLNGVIGFSDLLITEKPGSLNPAQKEYVGEILEGGKR
                     LLRLTDELLRVSRLRQRPLRKETVNTEGLVSEILRALRNAEPERAIEVHVGALPEASA
                     DPALLRQIFVNLLANAFKFTRRTPHPRVEITADQDAHGRTYRIRDNGAGFEMAQAHRL
                     FTIFQRLHPESEFEGSGVGLSIVQRIIERHGGTISAEGAVGAGATFTFTLPM"
     gene            complement(3262..4290)
                     /locus_tag="VFB37_09885"
     CDS             complement(3262..4290)
                     /locus_tag="VFB37_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZO22799.1"
                     /translation="MRTGTATRKLHPDPRVGLEARGSAPISSIGFLLLPGFSLMSYAS
                     CVEPLRAANVLAQRTLYRWWHAAPGNRPAAATSGAVLVPDLSLESKAPGIDLLLVCAA
                     GNPSTFNDRRILAWLRSLARRGVIIGGVSAGPFIMAKAGLLTGRRCTVHWEHMPALQE
                     AFPELRLTRTLFELDADRITCSGGVAGLDMMIALITRDHGYELGAEVSDWFLHTAVRE
                     GAGPQRMDLRFRLGIGDEKLLAVLKAMEANLEMPLSRQHLARIAGLSLRQLERQFHKG
                     VGRGLHAHYLALRLRRSRQLLRETSLSVLEVGVATGFASASHFSRAFRAAFGDVPRAA
                     RQRLTSAR"
     gene            4405..5655
                     /locus_tag="VFB37_09890"
     CDS             4405..5655
                     /locus_tag="VFB37_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HZO22800.1"
                     /translation="MRFSIFTLARQALAGHRGWTETWRDATPRSGYDVVIIGGGGHGL
                     ATAYYLARNHGISRVAVLEKGWIGSGNAGRNTTIIRSNYLLAGNNPFYEWSLRLWEGL
                     EQDLNYNTMVSQRGVLNLCHSDAQRDAYARRGNAMRMYGVDAELLDPEQVRRMLPMLD
                     FDNARFPIRGALLQRRGGTARHDAVVWGYARAASARGVDIIQNCEVTGFVRQGERICG
                     VQTTRGTIQADRVGMAVAGNTSRVGRFAGLQLPIESHVLQAFVTEAIKPFMHSVVTFG
                     GGHFYISQSDKGGLVFGGDIDGYNSYAQRGNLPTVEDVCEGGMALIPALGRVRMLRSW
                     GGVMDMTMDGSPIIDRTPIEGLYLNGGWCYGGFKATPASGWCFAHLLARGESHALARA
                     YRLDRFASGCLLDEKGLGAQPNLH"
     gene            5684..5956
                     /locus_tag="VFB37_09895"
     CDS             5684..5956
                     /locus_tag="VFB37_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HZO22801.1"
                     /translation="MRIPCPYCGEREVSEFAYLGDADCVRPDASAPDASLRYYEAVYL
                     RENPAGPHDELWFHFRACRSWLRVTRDTRTHEILGVVLAGNVAEAG"
     gene            5989..9006
                     /locus_tag="VFB37_09900"
     CDS             5989..9006
                     /locus_tag="VFB37_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HZO22802.1"
                     /translation="MTDPQQPNRIPRGGQIDRAQALRFRFDGRPYSGFAGDTLAAALL
                     AAGVRRVGRSFKYHRSRGVLTAGSEEPNALVELRLGARREANTRATVAELFEGLEASS
                     QNRWPSLALDVGAVSSLLAPLFVAGFYYKTFMWPRSFWERLYEPAIRRAAGLGRASGV
                     PDPDTYEKSHAFCDVLVIGAGPAGLACALEAARRGARVVLCDEDFLLGGRLNADRHSI
                     DGMEGAAWVRQVATELASCPDVKVLRRTTVFGTYDGTVGPRFPAGARTFGALERVSDH
                     LPVPPAFQPRQRLWKIVAHRTILAAGATERPIVFGGNDLPGIMLASAVRSYVNRFAVA
                     PGRRVAIFTASDDGWTTAFDLLAAGIPVAAVVDARAEPSSALAEKLRPSGTPLYAGGR
                     IVQGHGSSGLNGLTVLDRDSRRIRLSVDTLAVSGGWNPNLALATHLGGRPTWSEPLCA
                     FVPGSVPAGMTVVGAAAGAFTLAEALREGSRAGAQAAEDLGFSATASSQWRADEESSA
                     VTALWFVGGSKGKAFVDLQHDVTHEDVALAAREGFRSVELLKRYTTLGMATDQGRTSS
                     TNGHAIMAALTGRSIPELGNTTFRPPYTPVAIGAFAGHHRGKHFRPTRLTAGHRWAQE
                     RGATFIEAGEWLRAQWFAAPGDADWRSTVNREVQAVRTRVGLCDVSTLGKVDIQGADA
                     ALFLDRIYCNLFSTLPVGKVRYGLMLREDGFVLDDGTTAHLSAGHYVMSTTTVHAAKV
                     MQHLEYARQVLWPALDIQLASVTEQWSQYAIAGPESRRVLERLLDNSLDVSNTAFPYL
                     ACAEFRWRGVPARLFRISFSGELAYELAVPAQHGDAAIRAIAVAGEPFGMIPYGLESL
                     NVMRIEKGHVTGNELNGTTTAADLGMGRMMSTRKDFIGRVLAQRPGLTDPQRPALVGI
                     RPLDPRAPVRAGSHFLASGAAANLENDQGHVTSAAFSPMLGSWIALGLLKCGRERLGE
                     RIRIHDPLRKAPDVQAEVVSPVFFDPAAERPLA"
     gene            9003..9608
                     /locus_tag="VFB37_09905"
     CDS             9003..9608
                     /locus_tag="VFB37_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HZO22803.1"
                     /translation="MSAPRLETCSAFAKLPFSSAPGRGVIALDRPAVALASVLTRKAQ
                     SAGLRRRVFEAFGLELPEGPRYIAARRVAFVGTGIGAWLALAEADDPEFSTALGRALR
                     PAAAVADQSGAYAILRLSGPQVRSTLAKLLPLDLHPRVFEVGMAAATVAAHISIHLWR
                     LADGPQTLPVFELAVPRSLSVSFWHALSESAAEFGFALSPG"
     gene            complement(9595..11238)
                     /locus_tag="VFB37_09910"
     CDS             complement(9595..11238)
                     /locus_tag="VFB37_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZO22804.1"
                     /translation="MSATQLDSAPGFLTKDRIIAREGYSRWLVPPCALAIHLCIGMAY
                     GFSVFWLPLSKAVGITHSVACPADMGFFGEITSRTCDWKISMLGWMFTLFFVFLGSAA
                     AVWGGWLERAGPRKAGVVAAVCWCGGLLISAFGVITHQIWLLWVGSGAIGGIGLGLGY
                     ISPVSTLVKWFPDRRGMATGMAIMGFGGGAMIGSPLADRLMKFFATPTQVGVWQTFLV
                     LAAVYFVFMMAGALGYRVPPPNWKPAGWQGARKRALVTEGQVHVDVAWRTPQFWFLWA
                     VLTLNVTAGIGILGMASPLLQEVFGGRLIGVKASFDQLSQAQLGAIAAIAAGFTGLLS
                     LFNIAGRIFWASLSDTIGRQATYMVFFLLGIVLYASIPWTASTDSLALFVGFFCIILS
                     MYGGGFSTIPAYLADIFGEKMVGAIHGLILTAWSTAGVLGPVLVNYIREYQLDHGVPR
                     AQVYSITMYILAGLLALGFVCNLLVRPVDPKRYMTAAQIAALDKPADGSIPRTTSSSA
                     AIATTPTWLIYAAWLAVGIPLAWGVWVTLQKAAVMFSQG"
     gene            11592..>12396
                     /locus_tag="VFB37_09915"
     CDS             11592..>12396
                     /locus_tag="VFB37_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HZO22805.1"
                     /translation="MLIRSGIALAALCWMSGLSCAWAVQADQPPTAPAGVPKPPDPQS
                     SVSQAPIPGSTAPQTLPVLQNPVQTGTVPPQTPSEVVVEAPEPKYVAPTLRDRIGRIW
                     APVLINGKGPFRLVLDTGASHSAVIERVVDRLGLAGQEVTPIMVRGVTGSALVPAIHV
                     ESMEVGDLLIDPATLPIVADVFGGADGVLGREGLPDKRIFADFRHDRLVIARSHLERP
                     PAGYTPIHLKLTRGGLLQAEVRVGRVAADAIIDTGGQQTVGNVALREALM"
BASE COUNT         2118 a         4081 c         4120 g         2077 t
ORIGIN      
        1 cattctctgg ggtaccgaac gcaggcgcgc ccgagcgcat gtccacattg gaggtgaatg
       61 cgccgtaaac gattggggca cccgggcgca cgagctgggc aagtacggcg ccgaacagcg
      121 cttccgcatt ctgctgggcc agcgccgcgg caagcgatac cggcgccatg gctcccatga
      181 gggtgaacgg ggtgatcacg acggcctggg cgtgctcggc catcgtcatg aggccatcgg
      241 ccatcgcggc atcgaagcgg cgcgggctgt tgaccgaaat gacggtgatg acgcccgggc
      301 tctgcgccat ctgctccagc gacaggcccc gggcgatcgc catcatgcgg ataccatcca
      361 gtgccctgcc ggcgccgatg gcggtgcagt ggtagacccg atccgagtac agcaggttcg
      421 cccggtaggt atcgaggtga cgcgagttcg ccggcagctc caccggtgcg cacacctggt
      481 tgccgatcag gtgaatggcg ttgaagtact gcgcgagacg gatgaagtcg caatagtcgg
      541 agtagttacc gggccggcgg ccgcgctcac agtcatgaac cgagggcggc ccggccacga
      601 gcccgaaatt gatctgattg ccgcccagca cgacgcggtg agcggggttg cgcggcgtca
      661 gggtgaacga ggcgggcgca ctcgagagag cttgctgcac gagtccccgg tcgagtcgca
      721 ccaggcccgt atcggcatcc acgctcgcac ccgctgcgct gagcacggcc agcgcccgtg
      781 gggacatcac ttcgatgccc agctcttcga ggatgcgcag cgaagtcaga tggatggctt
      841 cgagctgatc ggccgacagc acctgtagcg gcggataggg attggtgacg tcgcgccacg
      901 ggagttgcgc aatcccacca gcggcgcgtc ggccgcgacc cccctggcgg cgccgggtcg
      961 tgttcgagtc catgcttgca gcatgctctg cccttcgcgt gcgccactca tgcacggatc
     1021 cgacatggcg gtgattgccg ccgacatcgc gccccagccg ccggcgcatt ggcgagagcg
     1081 gccggcctgt gccatcgttg cacgcgatgc ttcggagcca ggcgcagtgg tagtcaagcg
     1141 agcgggcgag cccgcgcgga tcgcattctt cctggtgcct cagttcccgc tgattgcgtt
     1201 tgccgccggg ctggagccgc tgcgccaggc gaatcgcatg agcgagcggc gtctgttcga
     1261 ctggccgctg gtctcggtgg atggcaaggc ggtcaccgcc agcaatggca ttccgatcgg
     1321 cgtgcacggg gcgctggata cgctgaacgg gatcgatagt gtggtggtct gcgccgggct
     1381 cgagccgacg cagctcggtc gccatcacaa ggcgcaccat catctgcggc ggctggcccg
     1441 ccacggcagc cggatcggcg cgatcagcac cggctcattc atcctggccg atgcggggct
     1501 gctcggcgac caccgctgta ccgtgcactg ggaatacacc gactcgctcc gctcccgcca
     1561 tccgggtctg aaactcaccc aggatctgta cgtcatcgat cggaacgtgt ttacctgctc
     1621 cgggggtacc tcggcgctcg acatgatgct gcagttcgtg agcgaggcgt cggggccgga
     1681 gcttgcgctg gcagttgccg agcagttcat ccacccgcag atccgccacc agcaggatca
     1741 gcagcgcatt ggcatgcacg cgcgctatgg gatcgacagc ccgaagctgg tcgagatcat
     1801 cacgctcatg gaaggggcga tcgaggaccc gctggaggta accgggatcg cgcagcacgt
     1861 cggggtttcg ccgcgccagg tcgagcggct ttttcgtgag catttgaata cctcgccgcg
     1921 ggcgttctac gttcgattgc ggctcactcg cgcacggacc ctgttgcgcc agacgctgaa
     1981 ttcggttcga gcggtcgcgg tcgagtgcgg gttcgcctcc gcctctcatt tctgtcacgc
     2041 ctacaagcgc gtattcggcc tctctccaac cgaggagcgg cagcgtctca ccggcggacg
     2101 cccgcggcga gagaccgatg gctgaccccg gcgtcacgcc gcatgcacgg attctcatcc
     2161 tcgatgagga gcaggctccg gtggcggcgc tgtgccggat gctgacgaca gaaggctacg
     2221 aggtgaccgc agtcagctcc ggatctgcgg ctctcgccgc gctgcgcgcg gccgttgccg
     2281 atcccgctgc acgattcgag gtgctcatct tcgacctgaa gaggcccgcg aaagatggcc
     2341 tcacgctgtt gcaggccgcc cgggaaatcg acgcggcgct cgaagccatc atcatgacgg
     2401 acccgggcca cctcgattcg gcgatgcagg ccctgcctgg tgcggcgctc gctcatattt
     2461 cgaagccgtt caagctcaac accctcgtgg cgggcctcgc gcgcgcgcag cagttccggc
     2521 ggctgcgcct ggaaaacaga gcgctcgcgg agcagatcgg gcagcgcaac gggcagctcg
     2581 agaaggccaa cctcgagctg ctgcgcgccg accaggctct cgaggcattc gttcactcgg
     2641 tctcgcacga tctgcgtcag ccgctcaatg gcgtgatagg tttcagcgac ttgctgatca
     2701 ctgagaagcc cggcagcctc aatccggcac agaaggagta cgtcggggag atcctcgaag
     2761 gtggcaaacg gctgctgcga ctgaccgatg agctgctgcg ggtctcgcgg ttgcgccagc
     2821 gacccctgcg caaggaaacc gtcaacaccg aagggctcgt gtcagagatc ctccgcgccc
     2881 tgcgcaacgc agaaccggag cgcgcgatcg aagtgcacgt gggcgcgctg cccgaggcgt
     2941 cggccgatcc cgcgctgctg aggcagatct tcgtcaatct gctggcgaat gccttcaagt
     3001 tcacgcggcg cacgccgcat ccacgagtgg agatcaccgc agaccaggat gcgcatgggc
     3061 gcacttaccg gattcgcgac aacggggccg gtttcgagat ggcgcaggcc catcggctgt
     3121 tcacgatctt tcagcgcttg cacccggaaa gcgagttcga gggcagcgga gtcggcctgt
     3181 cgatcgtcca gcgcatcatc gagcgccatg gcggcacgat ttccgctgaa ggcgccgtgg
     3241 gcgcgggcgc caccttcacg ttcaccttgc cgatgtgagc cgctgccggg cagcgcgcgg
     3301 cacatcgccg aaggctgcac ggaatgcgcg cgagaaatgg ctcgccgagg caaatcccgt
     3361 cgcaacgccg acttccagca ccgagagcga agtctcgcgc agcagctgcc gtgagcgccg
     3421 cagccgcaat gccagatagt gcgcgtgcag gcctcggccc acgcccttgt gaaactgccg
     3481 ctccagctgc ctcagcgaca gacccgctat ccgcgcaaga tgctgccgcg acagcggcat
     3541 ctccaggttc gcctccatcg ccttgagcac cgcgagcagc ttctcgtcac cgatgccgag
     3601 gcgaaatcgc agatccatgc gctgtggccc ggccccttca cgcacggctg tatgcagaaa
     3661 ccagtcactg acttcggcgc cgagttcgta gccatgatcg cgagtgatca gcgcgatcat
     3721 catgtcgaga ccggccaccc cgccggagca ggtgatgcgg tctgcgtcga gctcgaacag
     3781 cgtgcgggtc agcctgagct ccggaaaggc ttcctgcaga gccggcatgt gctcccagtg
     3841 aacggtgcag cgccgcccgg tcagcagccc ggctttggcc atgatgaacg gcccagcgga
     3901 aacaccgccg atgatcactc cgcgccgggc gagcgagcgc agccaggcga ggatgcgccg
     3961 gtcattgaaa gtggaaggat tgccggccgc gcacaccagc agcagatcga tccccggggc
     4021 cttcgactcc aggctcagat cggggacgag gacggcaccg ctggtggcgg cggcgggccg
     4081 gttgcccgga gcggcatgcc accagcggta cagcgtgcgc tgggcgagga cattggccgc
     4141 gcgcagcggc tccacgcacg aggcatacga catgagtgag aagccgggca gcaataaaaa
     4201 gccgatcgag ctgatcggcg ctgagccgcg cgcctcgagt cccacacgcg gatccgggtg
     4261 cagcttgcgg gtcgccgttc ccgtcctcat ctcgcgttgc cgttcatggt ggcgtggaat
     4321 gtagattttc cgccgcaaaa gatcaattgc ccgctcacgg ccgctctacc atcggcgccg
     4381 ggtcacgcgc tcgtggggag gtccatgcgc ttctcgatct ttacgcttgc ccgccaggca
     4441 ctcgccggac accgcggctg gaccgagacc tggcgcgatg ccacgccccg gtccggctac
     4501 gatgtcgtga tcatcggcgg cggcggccac gggctcgcca ctgcctacta tctcgcgcgc
     4561 aatcacggca tcagccgggt cgccgtgctc gagaaaggct ggatcggatc gggcaacgcc
     4621 ggacgcaaca ccacgatcat ccgctcgaac tacctgctcg caggcaacaa cccgttctat
     4681 gaatggtcgc tgcgactgtg ggaagggctc gagcaggatc tcaattacaa caccatggtg
     4741 agccagcgag gcgtgctgaa tctgtgtcac tcagatgcac agcgcgatgc gtacgcaaga
     4801 cgtggaaacg ccatgcgcat gtacggggtg gatgccgagt tgctcgaccc cgagcaggtc
     4861 cggcggatgt tgccgatgct cgatttcgat aatgctcgct ttcccattcg cggcgccttg
     4921 ctgcaacggc gcggcggcac ggcgcgtcac gatgcagtgg tgtggggata tgcgcgagcg
     4981 gcgagcgcac ggggtgtgga catcattcag aactgcgaag tcacgggctt cgtgcgccag
     5041 ggtgagcgga tctgcggcgt acagaccact cgcggcacca tccaggcgga cagagtgggc
     5101 atggccgtgg cgggcaacac ctcgcgcgtc gggcgattcg ccggactgca gctgcccatc
     5161 gagagccacg tgctgcaggc gttcgtcacc gaagcgatca aacccttcat gcacagcgtg
     5221 gtgactttcg gcgggggcca tttctacatc agccagtcgg acaagggtgg gctggtattt
     5281 ggtggcgaca tcgacggcta caactcctac gcacagcggg gaaatctacc cacggtcgag
     5341 gacgtgtgcg agggcggcat ggcgctgata cctgctctcg gcagggtgcg gatgctgcgc
     5401 tcctggggcg gtgtgatgga catgacgatg gacggctcgc cgatcatcga tcgcacgccg
     5461 atcgaggggt tgtacctgaa cggtggctgg tgttatggcg gattcaaggc cacgcccgcc
     5521 tcgggctggt gttttgcaca tctgcttgcg cgcggtgaat cgcacgcgct cgcccgcgcc
     5581 taccgcctcg accgctttgc cagcggctgc cttctcgatg agaagggact cggcgcccaa
     5641 cccaacctgc attgagcgcc ggcagagccc gcggagcaac cccatgcgca ttccatgtcc
     5701 atactgtggc gagcgcgagg tcagcgagtt cgcctatctc ggggatgcgg attgcgtacg
     5761 ccccgatgcc agcgcgcccg atgcatcgct tcgatactac gaggcggtgt atctgcgtga
     5821 gaacccggcg ggtccccacg acgagctgtg gtttcatttc cgggcctgcc ggtcgtggtt
     5881 acgcgttacg agggacacgc gcacgcacga gatcctggga gtcgtcctgg ccggcaacgt
     5941 ggcggaggcc ggatgagcgg gccggagcag ccgatcggag ctgagcgcat gaccgatccg
     6001 cagcaaccca acagaattcc ccgcgggggc cagatcgatc gggcgcaagc cctgcgcttt
     6061 cgcttcgatg gccgccccta cagcggattt gccggcgata cgctcgccgc ggctctgctg
     6121 gcggccggag tccggcgcgt gggccgctcc ttcaagtatc accgctcgcg cggagtgttg
     6181 acggccggct cggaagagcc caacgcactg gtcgagctgc gcctcggcgc tcgccgtgaa
     6241 gccaacaccc gggccacggt ggcggagctc ttcgaggggc tcgaggcgag cagccagaac
     6301 cgctggccct ctctggcgtt ggacgtcggc gccgtgagct cgctcctcgc gccactcttc
     6361 gtcgccggct tctactacaa gaccttcatg tggccgcgct cgttctggga aaggctgtat
     6421 gagccggcga ttcgccgggc ggcaggcctc gggcgcgcga gcggtgtgcc cgatccggac
     6481 acgtacgaga aatcccatgc attctgcgac gtgctggtca tcggagcggg gccggcaggt
     6541 ctcgcttgcg ccctggaagc ggcgcgtcgc ggcgcgcgcg tcgtgctgtg tgatgaagac
     6601 tttctgctcg gcgggcggct caatgccgat cggcattcca tagacgggat ggagggtgcc
     6661 gcgtgggtac gccaggtagc gactgagctc gcatcctgtc ccgatgtcaa ggttttgcgc
     6721 cgcacgactg tgtttggaac ctacgatggc acggtgggcc cgcgcttccc ggcgggcgcc
     6781 cgcaccttcg gcgcgctgga gcgagtatcg gaccacctgc cggtgcctcc cgcgttccag
     6841 ccgcggcagc gcctttggaa gattgtcgcc catcgcacca tcctcgcggc gggggcgacc
     6901 gagcggccca tcgttttcgg aggcaacgac ctgccgggca tcatgctggc ctccgctgtg
     6961 cgttcgtatg tcaatcgttt cgcggttgcc ccgggtcgac gcgtcgcgat cttcacggcc
     7021 tcagatgatg gctggacgac ggcattcgat ttgttggcgg cgggtatccc ggttgctgcc
     7081 gtcgtcgatg cgcgcgccga gccctcttcc gcactcgccg agaaattgcg gcccagcggc
     7141 accccgctct atgcgggagg gcggatcgtg caggggcacg gaagcagcgg tttgaatggt
     7201 ctcaccgtcc tggaccggga ctcacggcga atccggttgt ccgtggacac cctcgccgtc
     7261 tccggcggct ggaatccgaa cctggcgctc gcaacccacc tggggggccg acccacatgg
     7321 tcggagccgc tctgtgcctt cgtgccgggc agcgtgcccg ccgggatgac ggttgtcggg
     7381 gccgcggcgg gcgctttcac cctggccgaa gccttgcgcg aaggctcccg ggcaggagcg
     7441 caggcggcgg aggatctcgg attttccgcc acagcgagct cgcaatggcg cgcagatgag
     7501 gaaagcagcg ccgtcacggc cctgtggttc gtcggtgggt cgaaaggcaa ggcgttcgtt
     7561 gatctccagc acgatgtgac ccacgaggat gtcgcgctcg ccgcgcggga gggctttcgc
     7621 tccgtggagc ttctcaagcg ctatacgacg ctcgggatgg ctaccgatca gggacgcacc
     7681 tcgagtacca acgggcacgc gatcatggca gcgctcacgg gccgctcgat tccggagttg
     7741 ggcaacacga cgttccgtcc gccctacacg cccgtggcca tcggtgcctt tgccggtcac
     7801 catcgcggca agcacttcag gcccacccgc ctgacggccg gccaccgctg ggcgcaggaa
     7861 cggggggcga ccttcatcga ggccggagag tggctgcgcg cgcaatggtt tgccgcaccg
     7921 ggcgatgccg actggcgctc gaccgtgaat cgcgaggtcc aggccgttcg cacgcgtgtc
     7981 ggcctttgcg acgtctccac gctgggcaag gtggacatcc agggagccga tgcggccctg
     8041 ttcctcgatc ggatctactg caacctcttc tcgacgctgc ccgtgggcaa ggtgcgctat
     8101 gggttgatgc tgcgcgagga tggattcgtt ctggatgacg gtacgaccgc gcacctctct
     8161 gccggtcact acgtcatgtc cacgaccacc gtccatgcag cgaaggtgat gcagcacctg
     8221 gagtacgcgc gacaggtcct ctggcccgct ctcgacatcc agctcgcctc ggtcaccgag
     8281 cagtggtcgc agtacgcgat tgcaggaccc gagtcccgcc gcgtgctcga gcggctgctc
     8341 gacaactcgc tcgatgtttc caacactgcc tttccctatc tcgcctgcgc ggagttccgc
     8401 tggcgagggg tgccagcgcg actgttccgc atctccttct ccggcgagct cgcctacgag
     8461 ctcgccgtgc cggcgcagca tggcgatgcg gccatccgcg cgatcgcggt tgctggcgag
     8521 cccttcggca tgattccgta cgggctcgag agtctcaatg tcatgcgcat cgagaaagga
     8581 catgtgaccg gcaacgagct caatggcacg acgaccgccg ctgacctcgg catgggccgc
     8641 atgatgtcca cacgcaagga cttcatcggc cgagtgctcg cccagagacc ggggctcacc
     8701 gatccgcagc ggcccgcact ggtcggcatc cgcccgctcg atccacgcgc ccccgttcgc
     8761 gctggcagcc atttcctggc atcaggcgca gcggcaaacc tcgagaacga tcagggtcac
     8821 gtcacttcgg cggcgttctc gccgatgttg ggatcgtgga tcgccctggg gctactcaag
     8881 tgcggacgcg agcgcctcgg agagcgcatt cgcatccacg atccgctgcg caaagcgccg
     8941 gatgtgcagg cggaagtcgt ctcaccggtt ttcttcgacc ccgccgccga gaggccgctc
     9001 gcatgagtgc accgcgactc gagacttgct cagccttcgc gaagctgccc ttcagttccg
     9061 ctccgggccg cggagtgatc gccttggatc gtccggccgt ggcgctcgcg agcgttctca
     9121 ccagaaaggc gcagagtgcc gggcttcgcc gccgcgtatt cgaggcattc ggcctcgagc
     9181 tgccggaggg tcctcgatac atcgcagcgc gaagagttgc attcgtcgga accggaatcg
     9241 gagcctggct ggcgctggcc gaagcggatg accctgagtt cagtaccgcg ctgggccgcg
     9301 cgctccggcc agctgccgcc gtggcggacc agagtggcgc ctacgcgatt ctgcgcctga
     9361 gcggcccaca agtgcggagc acactcgcaa agctgctgcc actcgatttg catccgcgcg
     9421 tgttcgaggt tgggatggcc gccgccacgg tggcggccca catctccatt cacctgtgga
     9481 ggctcgcaga cggtccccag accctgcctg tgttcgagct ggccgtacct cgcagcctca
     9541 gcgtcagctt ctggcatgcg ctgagcgaga gtgccgcgga gtttggtttc gcgctcagcc
     9601 ctggctgaac atcaccgcag ccttctgcaa agtcacccaa acgccccagg ccaaggggat
     9661 tcccaccgcc agccaggctg catagatgag ccaggtcgga gtggtcgcta tcgccgctga
     9721 cgagctcgtc gtccgtggga tcgagccgtc cgcgggctta tcgagcgcgg caatctgcgc
     9781 ggccgtcatg tagcgcttcg ggtccaccgg gcgcaccagc aggttgcata cgaaacccag
     9841 ggccagcagc cccgccagga tgtacatcgt gatgctgtat acctgggcgc gcggcacacc
     9901 atgatcgagc tggtattctc gaatgtagtt caccagcacc ggcccgagca ccccggcggt
     9961 ggaccaggct gtcaggatga gcccgtggat cgcgccgacc attttctcgc cgaagatgtc
    10021 cgccaggtac gcgggaatcg tcgagaatcc tccgccgtac atcgagagaa tgatgcagaa
    10081 gaatccgacg aagagcgcga ggctgtcggt cgaagccgtc caggggatgg aggcgtacag
    10141 cacgatgccg agcaggaaga acaccatgta tgtcgcctgg cgtccgatcg tatccgacag
    10201 cgaagcccag aagatccgcc ccgcgatgtt gaacagcgag agcagtccgg tgaagcccgc
    10261 ggcgatcgcg gcgatcgcgc cgagctgtgc ctgcgagagc tgatcgaacg aggccttgac
    10321 gccgatcaac cgcccgccga agacctcctg caacagaggc gaggccatgc ccaggatgcc
    10381 gatccccgcg gtcacgttca gcgtgagcac ggcccacagg aaccagaact gcggcgtgcg
    10441 ccaggcgaca tcgacatgga cctgcccctc ggtgacgagc gcgcgtttgc gcgcgccctg
    10501 ccagcccgcc ggcttccaat tcggcggcgg cacgcgatag ccgagcgcgc cggccatcat
    10561 gaacacgaag tagaccgccg ccagcaccag gaaggtctgc cagacgccca cctgagttgg
    10621 cgtagcgaag aacttcatca gacggtccgc cagcggggag ccgatcatgg ccccgccacc
    10681 gaaacccatg atggccatgc cggtcgccat gccgcgacgg tccggaaacc acttgaccag
    10741 cgtggatacc ggtgagatgt atccgagccc gagaccaatg ccgccaatgg cacccgagcc
    10801 gacccacaac agccagatct gatgggtaat gaccccaaag gccgagatga gcagtccgcc
    10861 gcaccagcac actgcggcca cgacgccggc tttgcgcggc ccggcgcgct cgagccaccc
    10921 gccccacacc gccgcagccg acccaagaaa tacgaagaac agggtgaaca tccaccccag
    10981 catggagatc ttccagtcgc atgtcctgga ggtgatctcg ccgaagaagc ccatatctgc
    11041 cggacaggcg accgaatgag tgatgccgac ggccttggac aggggaagcc agaaaacgga
    11101 aaagccataa gccatgccga tgcaaaggtg aatggccagc gcgcagggcg ggaccagcca
    11161 gcggctgtag ccttcccggg caatgatgcg gtcctttgtc aaaaacccgg gggccgaatc
    11221 cagttgcgta gcggacatgt tgcggctccg ttgtctcgag gcgtgggtca gctcgttgtg
    11281 gtacttggct gcggcgcgga ggctgttggc gtgattggtg ttgtgattcc ggatagtgca
    11341 ggggcagccg cgccgcccga tgatatcgcc atcatttttg gggatgcaaa tgacaggacg
    11401 tgcagcggca tcccggcgca tcgcatgccc gagcgcaccc gcagagtact tcacaagagt
    11461 gtcggtgtac ggccgactgt ctcagcgtgg cagatgccgg tacactgtgc cgaattcgat
    11521 acgcgggggc gtcagcgcca gcgaggcaga acgcacggcg ccggagtcat ggactcgcgg
    11581 ggcagatcac gatgttaatt cgaagcggaa tcgcgctggc agcgctctgt tggatgagtg
    11641 gactgtcctg cgcatgggcc gtccaggccg accaaccgcc cacagccccg gccggcgttc
    11701 ccaagccccc ggacccccag agctcggttt cccaggcacc catcccgggc tcaacggctc
    11761 cgcagactct gccggtcctg caaaatcccg ttcaaacggg cacggttcct ccacagacgc
    11821 cctcggaggt ggtcgtcgag gcgcccgagc cgaagtacgt cgctcccacc ctccgcgacc
    11881 gtatcgggcg catctgggcg cccgtgctga tcaacggcaa gggtcccttt cgcctggtgc
    11941 tcgacaccgg cgcgagccat tcggccgtca tcgagcgcgt cgtggaccgg ctcgggttgg
    12001 ccggtcagga ggtcacgccg atcatggttc gtggcgtcac cggatccgcc cttgtcccgg
    12061 ccatccatgt cgagagcatg gaggtggggg atcttttgat cgatccggcc acattgccaa
    12121 tcgttgccga cgtgtttggg ggcgccgatg gcgtgctcgg acgcgaaggg ctgccggaca
    12181 aacgcatctt tgccgatttt cgccatgacc ggctggtgat cgcgcgctct cacctcgagc
    12241 gccctccggc cggctacacc cccatccacc tcaagttgac tcgtggcggc ttgcttcagg
    12301 ccgaggtgcg ggtgggcaga gtcgcggccg atgccatcat cgacactggc gggcagcaga
    12361 cggtgggcaa cgttgcgctg cgcgaagccc tcatga
//
LOCUS       DASRHN010000248         4254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_413161, whole genome shotgun sequence.
ACCESSION   DASRHN010000248 DASRHN010000000
VERSION     DASRHN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413161"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..721)
                     /locus_tag="VFB37_09920"
     CDS             complement(293..721)
                     /locus_tag="VFB37_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22806.1"
                     /translation="MESKIRHQLELLPERQSPHVEPVTSELIQTIKRRQTFLAAWNFA
                     QDFAGLEHKEVYVPLGIDAAHYTKIRNGTASPPADERFNRYLEIVGNEIPLIWWAESR
                     GYDWLTIRKHRSNEQRRIAELEAENQDLKRAFALAFGQRK"
     gene            816..1406
                     /locus_tag="VFB37_09925"
     CDS             816..1406
                     /locus_tag="VFB37_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22807.1"
                     /translation="MIEREPCFLGDAFQQIHGRRMHSSQDVADGRLANADPPRELCLR
                     GIRRSEVGLKRLHMSGKSIGNAYRIAIGQTYMESRHADFMAKIRSVLDRAMEALAERY
                     PRQKPTQERLAALAGVKQPSVNEWKDGFPAMENAVRLSENLGICVEWLLTERGPKHPP
                     RAPKDEIGTLSTIWPQLDERKKAEVTRFADFLKEDK"
     gene            1669..1989
                     /locus_tag="VFB37_09930"
     CDS             1669..1989
                     /locus_tag="VFB37_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22808.1"
                     /translation="MVRSSKQNWQVGSRVRVGFLNLVIRAAIATPGDARPDTYVLSNA
                     AGDKLYAFTPHHGCERISMHEARELLETAQAQAARVAQQALREAARRSQAAAAHDALF
                     AEVA"
     gene            1989..2261
                     /locus_tag="VFB37_09935"
     CDS             1989..2261
                     /locus_tag="VFB37_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22809.1"
                     /translation="MNAAPAFAMDAQFVAELSTIKTVMEEAAAAMALATEQIAQLTRA
                     NARMATALRDIAEEADDRYDGAPDAGPMARWAGHLMSLALRGLGEA"
     gene            2261..2683
                     /locus_tag="VFB37_09940"
     CDS             2261..2683
                     /locus_tag="VFB37_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22810.1"
                     /translation="MDARTPYTALVERLADETLEPHCGVLGAQHLPTLSREDLATVLA
                     YEPTVVAEAAESRALASSLIESLRAESDCIARAACIGAAFQGSLHAQAKHFLLGDVLA
                     ELEARAERERQELDEEAREHGGFEESAEGRHGVAGMFR"
     gene            2680..3015
                     /locus_tag="VFB37_09945"
     CDS             2680..3015
                     /locus_tag="VFB37_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22811.1"
                     /translation="MSLHALIAEPADLAPPRAVITLAPTRTRPRFLTLKAVGDTLIAI
                     LQVRERAGLRTVLFSQAPLRQCRLDTRTPDPCLWMQSAALELAPEEAERLVRWGARAD
                     VHLATENTR"
     gene            3012..3218
                     /locus_tag="VFB37_09950"
     CDS             3012..3218
                     /locus_tag="VFB37_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22812.1"
                     /translation="MSSPERIAASVIAALAREPRWLNELDRARLDLQQRREERVRAEA
                     SLEAARRKEHETAVRLMKLTEEHL"
     gene            3215..3358
                     /locus_tag="VFB37_09955"
     CDS             3215..3358
                     /locus_tag="VFB37_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22813.1"
                     /translation="MRLLRMLFGRGDDVRLAVIRHLTTDLRNPRDAHRLRRKFEQRKV
                     LPS"
     gene            3355..3561
                     /locus_tag="VFB37_09960"
     CDS             3355..3561
                     /locus_tag="VFB37_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22814.1"
                     /translation="MSETRIYLVNDGHQQRLIRAPNAAQAIRYAAKDFTAEVASQDDL
                     CTLLVKGTRVEESSANGHAEPKDA"
     gene            3558..>4254
                     /locus_tag="VFB37_09965"
     CDS             3558..>4254
                     /locus_tag="VFB37_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HZO22815.1"
                     /translation="MNAVAAIRSRVPAEEYLASEGTSITRLKELRRSPQHYRYFLEHP
                     KQTQPLLLGTAAHVAVLEPERFEQQFAVWTRRSEKTGNLCPRNGQHWDAFQAQHGGKQ
                     ILTEDECSIALTIGSVVRADPVASKYLQSGEPEVVMEWELHGRRCRGRVDWLTLIEGE
                     PVIVGLKTTRDCRPFIFGAQCAKLGYHLQFAWYQAGYRVITGRKAKVVEIVVESTPPH
                     AVIVYRVPEDVILQ"
BASE COUNT          822 a         1333 c         1365 g          734 t
ORIGIN      
        1 agccccgccg caaactgaca tccgcatcgc ggacacacgc gcaaccatgt cattgctggt
       61 tggccgttat agatccggcg ccaaagagca cggctcagat cccatcgata cacaggcttc
      121 gcgaggcata gcagttcggc gttagcgctc aggggagatc cctccctgtt atgtcacacc
      181 gctgcccttc atgattctta agctcctgca cattccgggg ttgagatatc ggcgtacgct
      241 cagccagaga gacatacgag tgcggatcga ataacaagaa gggatgcacg ctctacttcc
      301 tctgcccgaa cgccagcgca aaggcgcgtt tgaggtcctg gttttcagct tcgagctcgg
      361 cgatccgccg ctgctcattc gatcggtgct tgcggatcgt cagccagtca tagccgcgcg
      421 actccgccca ccagatcaga gggatttcgt tcccgacgat ctccagatac cggttgaagc
      481 gctcatccgc cggcggcgag gccgttccgt tgcggatctt ggtgtagtgg gccgcatcga
      541 tcccaagcgg cacgtagact tccttgtgct caagacctgc gaagtcctgc gcgaagttcc
      601 aagctgccag gaacgtctgg cggcgcttga ttgtttgaat aagctcagaa gtgaccggct
      661 cgacatgcgg ggactgccgc tccgggagca gttccagctg gtgccgaatt ttggactcca
      721 ttggaatatg ctgctgctac gcaaaaaaaa ggggggcgac taccatggac tcaagaacct
      781 caagaaagcg gtcgcgttgt ctcaggcgcg agcagttgat cgagagagag ccctgtttcc
      841 tgggcgatgc gtttcaacaa attcacggac ggcgaatgca ctcctcgcaa gatgtcgctg
      901 atggtcggct ggctaacgcc gacccgccgc gcgaactctg cctgcgtggt attcgtcgat
      961 ctgaggtagg actcaagcgc ctgcatatgt ccggcaaatc tatcggaaat gcctatagga
     1021 ttgcaatagg ccaaacctat atggaatcgc ggcatgctga tttcatggca aaaattcgtt
     1081 ccgtactcga tcgagcgatg gaagcgctgg ctgagagata cccgaggcag aaaccgacgc
     1141 aggagcggct ggcagcgctc gccggggtaa aacaaccgtc ggtcaacgaa tggaaggacg
     1201 gttttccggc tatggagaat gccgtccgcc tctcagagaa cttggggatc tgtgtggaat
     1261 ggttgctcac agagcgtgga cccaagcatc caccgcgggc accaaaggac gagatcggca
     1321 ctctctcgac gatctggcca cagcttgatg agcggaaaaa ggccgaggtc acccgctttg
     1381 ccgattttct gaaagaagat aaatagggcc cggcccttga gagcttctcc acaggtagcg
     1441 gcgagcgatt cgattccatc ttccaccagc tagaaacggt cagtcgtcct gcgcggctcc
     1501 tggcagtcga gcagtggccg gtggatcctt caacatgccc tgactcttga gaacgaagct
     1561 atatcgcagc tcaaggccaa atatttctgc cgaaatatag gttttgccta ttgcattcac
     1621 tataggagac gcctatagtc cctcccatcg ggcattcggg aggttcaaat ggtccgcagc
     1681 agcaagcaga actggcaggt cggctcgcga gttcgggtcg gcttcttaaa cctggtcatc
     1741 cgcgctgcca tcgccacccc gggggatgcg cgccccgaca cgtatgtgct gagcaacgcc
     1801 gccggcgaca agctctacgc cttcaccccg caccacggct gcgagcgcat ctcgatgcac
     1861 gaggcgcgcg agctgctcga gaccgcgcag gcgcaggccg caagggtcgc ccagcaggcc
     1921 ctgcgcgagg ccgcccgccg ttcccaggcc gctgccgcac atgatgcgct cttcgcggag
     1981 gtggcctgat gaacgccgcc cccgccttcg ccatggacgc gcagttcgtc gccgaactga
     2041 gcacgatcaa gaccgtcatg gaggaagccg ccgccgccat ggcgctcgcg accgagcaga
     2101 ttgcgcagct cacccgcgcc aacgcgcgca tggcgacggc gctgcgtgac atcgccgagg
     2161 aagccgacga ccgctacgac ggggcgcccg atgcgggccc catggcgcgc tgggcggggc
     2221 atctcatgag cctcgcgctg cgcgggctgg gggaggcgtg atggacgccc gcaccccgta
     2281 caccgcgctc gtcgagcgcc tggccgatga gacgctggag ccgcattgcg gcgtcttggg
     2341 cgcgcagcat ctgccgacgc tcagccgcga ggacttggcc accgtgctgg cgtacgagcc
     2401 gacggtggtg gctgaggcgg ccgaatcccg tgcgctcgcg tcctcgctga tcgagtcctt
     2461 gcgcgccgag tcggactgca tcgcccgcgc cgcctgcatc ggggctgcgt tccaaggctc
     2521 gctgcacgcg caggccaagc atttcctgct gggcgatgtg ctggcggagc tcgaggcgcg
     2581 tgctgagcgg gaacgccagg aactcgacga ggaagcccgc gagcacggcg gcttcgaaga
     2641 aagcgccgaa ggccgccacg gcgtcgcggg gatgttccga tgagcctgca cgccctgatc
     2701 gccgagcccg ccgatctcgc cccgccgcgt gcggtgatca cgctggcgcc gacgcggacc
     2761 cggccgcgct ttctgacctt gaaagccgtc ggcgacacgc tgattgccat tctgcaggtg
     2821 cgcgagcgtg cggggttgcg caccgtgctc ttctcccagg ccccgttgag gcagtgccgc
     2881 cttgataccc gcacgccgga cccgtgcttg tggatgcagt cggcggcgtt ggagttggcc
     2941 ccggaagaag ccgagcggct ggtgcgctgg ggcgcccggg ccgatgtgca cctcgccacc
     3001 gagaacaccc gatgagttcg ccagagcgca tcgccgcctc cgtgattgca gcactggcgc
     3061 gcgagccgcg ctggctgaat gaactggacc gtgcgcggct ggatttgcag cagcgccgcg
     3121 aggaacgcgt ccgggcggag gcgagcttgg aagcggcgcg ccgcaaagag cacgagacgg
     3181 cggtaaggct gatgaaactg acggaggagc atctgtgaga ctgctacgaa tgctgtttgg
     3241 ccgtggcgat gacgtgcgac ttgccgtgat ccgccacctg accaccgacc tgcgcaaccc
     3301 gcgcgatgcc caccggctgc ggcgcaaatt cgaacaacgc aaggtgttgc cctcatgagc
     3361 gaaacccgga tctatctcgt caacgacggc catcagcagc gcctgattcg cgcgccgaac
     3421 gctgcgcagg cgatccgcta tgccgcgaag gacttcaccg cggaagtggc cagccaggac
     3481 gacctatgca cgctgctggt gaaaggcacc cgcgtcgagg agagcagcgc gaacggccac
     3541 gcggagccga aggacgcatg aacgccgtag ccgccatccg ctcgcgcgtc ccggccgagg
     3601 aatacttggc gagcgaaggg acgtcgatca cgcggctcaa ggagttgcgt cgctcgccac
     3661 agcactatcg ctatttcctc gagcatccca agcagacgca gccactgctg ctgggtaccg
     3721 cagcccatgt agcggtgttg gaacctgagc gctttgagca gcaattcgcc gtctggactc
     3781 ggcgcagcga gaagacgggc aacttgtgtc cgcgcaacgg gcagcactgg gatgccttcc
     3841 aggcacagca cggtggcaaa cagatcctga ctgaggacga atgcagcatt gctctcacca
     3901 tcggcagcgt cgtgcgcgcc gatccggtgg cgagcaagta cctgcaaagt ggagagcccg
     3961 aagtcgtcat ggaatgggag ctgcacgggc gacggtgccg cgggcgcgtg gactggctga
     4021 cattgatcga gggcgagcca gtgattgtcg ggctcaagac gacgcgcgat tgccgcccat
     4081 tcatcttcgg cgcccagtgc gccaaattgg gctatcacct gcaattcgcc tggtaccagg
     4141 ccggctaccg cgtgatcacg ggacggaagg cgaaggtagt cgagattgtg gtcgagtcaa
     4201 cgccgccgca tgcggtcatc gtctaccgcg tccccgagga tgtcattttg cagg
//
LOCUS       DASRHN010000249         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2062004, whole genome shotgun sequence.
ACCESSION   DASRHN010000249 DASRHN010000000
VERSION     DASRHN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062004"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VFB37_09970"
     CDS             complement(<1..1845)
                     /locus_tag="VFB37_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO22816.1"
                     /translation="MNPSRLFVLRPVATTLLMVAILVVGMIAYVQLPLSALPEVAYPT
                     IQVQTFYPGASPEVITSAITAPLEKQFGQMPGLDQMSSTSSAGASVITLTFTLSLDID
                     VAEQEVQAAISGAQNLLPQDLPAPPIYAKVNPADAPVLILGITSQVMPLTQVEDMADT
                     RIAQKISQLKGVGVVSISGGQRPAVRVIVNPRALAAYGLNLDDLRTTINVANQNGPKG
                     TLDGPSRAYTINTNDQIKDAAEYGSIVIAYRNGSPVRLRDVATLAAGAENTKLGGWMD
                     ATAALIVNVQRQPGANVVDVVNRVQAMLPTLQASLPPGVDVSVLTDRTTTIRASIRDV
                     ELELALAVILVVLVIYVFLRNVPATLIPSLSVPLSIIGTFAVMYLASFSLNNLSLMAL
                     TIATGFVVDDAIVMIENIARFIELGEPPLEAALKGAGQIGFTIISLTVSLIAVLIPLL
                     FMQDVVGRLFREFAVTLAVTILISALVSLTLVPMMCAKLLRHRPPSQRQADASGWFAA
                     LVSWYGRRLSWVLDHQRLTLYVAIATLAVTAALYVLIPKGFFPAQDTGLIQGVSEATE
                     SISYSAMAERQQALAAAILKDPDVASLSSFIGVDGNNVTLNSGRFLINL"
     gene            complement(1855..>2697)
                     /locus_tag="VFB37_09975"
     CDS             complement(1855..>2697)
                     /locus_tag="VFB37_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO22817.1"
                     /translation="DLKRFEQLITEDSVAQQQLDTQRALVDQYTGTVESDQATVDSAR
                     VNLQYTHIISPVTGRVGLRQVDQGNYVTPGDANGIVVVTQLQPITAIFSVPEDNVTVL
                     MQRLHAGATLKAEAYDRTNSTKLAEGKVLSVDNEIDTTTGTIKLRALFDNQDNLLFAN
                     QFVNIQLVVNELKNQTVIPASAVHRGAPQGVTSTFVYLVNNDSTVSVRPVTLGVVDGE
                     RVAIDKGLKAGDVVVTEGGDRLRDGAPVLLPANTPQHAQQPGARPPGSKSGRRNRAHA
                     QQPE"
BASE COUNT          494 a          806 c          902 g          495 t
ORIGIN      
        1 gaggttgatg aggaagcgtc cgctgttgag cgtgacgttg ttgccatcca caccgatgaa
       61 cgaggacagg ctcgcaacgt ccggatcctt gaggatcgct gccgcgaggg cctgctgacg
      121 ctcggccatg gcggagtagg agatcgactc ggtagcctcc gagacgccct gaatgaggcc
      181 ggtatcctgg gccggaaaga accccttagg aatgagaaca tacagggcgg cggtcaccgc
      241 gagtgtcgcg atcgccacat agagcgtcag tcgctggtgg tccagtaccc agctcagccg
      301 gcgcccatac cagctcacga gagccgcaaa ccagccggag gcatccgcct gtcgctgcga
      361 tggcggccgg tgccgcagga gcttcgcgca catcatcgga accagggtca atgagacgag
      421 cgccgagatc agaatggtca cggcgagcgt gacggcaaac tcgcggaaca gcctgcccac
      481 gacatcctgc atgaacagca gaggaatgag caccgcgatc agcgagaccg tcagcgagat
      541 gatggtgaag ccgatctggc cggctccctt gagtgccgcc tcgaggggtg gctcgcccag
      601 ctcgatgaaa cgagcgatgt tctcgatcat cacgatggca tcgtcgacta cgaagccggt
      661 ggcgatcgtg agcgccatca gcgagagatt gttcagactg aagctggcca ggtacatcac
      721 cgcaaacgtg ccgatgatcg acagcggaac ggacaggctc ggaatcagcg ttgccgggac
      781 gtttcgcagg aatacataga tcacgagcac gacgaggatc accgcgaggg cgagttccag
      841 ctcgacatcg cggatggaag cgcgaatggt ggtcgtgcga tcggtaagaa ccgaaacatc
      901 cacgcccggc ggcagcgagg cctgcagagt gggaagcatg gcctgcaccc ggttcaccac
      961 gtcgacgacg ttcgctccgg gttggcgctg cacgttcacg atgagcgcgg cagtggcatc
     1021 catccagccg ccgagcttgg tgttctcggc acctgcggcc agtgtcgcga cgtcccgcag
     1081 gcgtacgggc gagccgttgc gataggcgat gacgatgctc ccgtattcgg cggcgtcctt
     1141 tatctgatca ttcgtgttga tcgtgtacgc gcgcgagggg ccatccaggg ttcccttcgg
     1201 cccgttctga ttggcaacat tgatcgtcgt gcgcaggtca tcgaggttca gaccgtacgc
     1261 cgcgagtgcg cgcggattga cgatgaccct gacggccggg cgctgcccgc cgctgatgct
     1321 gaccacacca acgcctttga gctgcgagat tttctgcgcg atgcgcgtat ccgccatgtc
     1381 ttccacttgc gtcaacggca tcacttgcga agtaataccg aggatcagca ccggggcgtc
     1441 cgctgggttg accttggcgt agatcggtgg cgcaggcagg tcctgaggca gaaggttctg
     1501 ggctccgctg atcgccgcct ggacctcctg ctcggcgacg tcgatgtcca gggataaggt
     1561 gaaagtgagc gtgatcaccg aagcgccggc agagctggtc gaggacatct ggtcgagccc
     1621 cggcatctga ccgaactgct tctccagggg cgccgtgatg gcggaggtga tgacttcggg
     1681 gctcgcgccc ggatagaagg tctgaacctg gatggtgggg taggcgacct caggcagtgc
     1741 cgacaggggc aactgcacat aggcgatcat gccgaccacg aggatcgcca ccatcagcag
     1801 cgtagtggcg accggacgca ggacgaagag gcgcgacgga ttcatgccac gccgtcactc
     1861 gggctgctgc gcgtgcgctc ggtttcgccg cccgctcttg gagcccggtg ggcgcgcgcc
     1921 cggctgctgc gcatgctgcg gggtgttggc tggcagaagc acgggagcgc cgtcgcgcag
     1981 ccggtcaccg ccttcggtca cgacgacatc gcctgccttg agccctttgt cgatggccac
     2041 gcgctcgccg tcgaccacgc cgagtgtcac gggccgtaca gagacggtgc tgtcattgtt
     2101 cacgaggtat acgaaggtgc tggtgacgcc ctggggtgca ccgcggtgca ctgccgacgc
     2161 cggaatgacg gtctgattct tgagctcatt cacgacgagc tgaatgttca cgaactggtt
     2221 cgcgaacagc aggttgtcct gattgtcgaa cagcgcgcgc agtttgatcg tgcccgtcgt
     2281 ggtatcgatc tcattgtcca cgctgagcac cttgccttcg gcaagcttgg tgctgttggt
     2341 gcggtcatac gcttcggctt tgagagtggc tcccgcgtgc aggcgctgca tgagcaccgt
     2401 cacgttgtcc tccggcacgg agaagatcgc cgtgatcggc tgcagctggg tcacgaccac
     2461 gatgccgttc gcatcaccgg gggtgacgta gttgccctgg tccacctgcc gcaggccgac
     2521 tctgccggtc accggtgaga tgatgtgcgt gtattgcagg ttcacccgtg ccgaatcgac
     2581 cgtggcctga tcggattcga ccgtaccggt gtactggtcc acgagcgcgc gctgggtatc
     2641 cagctgctgc tgggccaccg agtcctcggt gatgagttgc tcgaagcgct tcagatc
//
LOCUS       DASRHN010000250         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_246577, whole genome shotgun sequence.
ACCESSION   DASRHN010000250 DASRHN010000000
VERSION     DASRHN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246577"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="era"
                     /locus_tag="VFB37_09980"
     CDS             <1..605
                     /gene="era"
                     /locus_tag="VFB37_09980"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZO22818.1"
                     /translation="ALKWTEADQQVLQRIVRSGLPAIAAVNKVDRVRPRARLLPYLEQ
                     LSARHTFVELVPLSALKTDNVDELRRVIAAHLPVAQPMFAQDELTDRSLEFRIAEMIR
                     EKLTLELNQEVPYGVAVEVEQLREQDEQLMVDAAIWVDREGQKPIVIGAGGERLKRVG
                     RSARLALNGLLGRRLHLNLWVKVRENWADNARALKQLGLE"
     gene            602..1372
                     /gene="recO"
                     /locus_tag="VFB37_09985"
     CDS             602..1372
                     /gene="recO"
                     /locus_tag="VFB37_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZO22819.1"
                     /translation="MTRSARRITLAPAYILHHRPYRDTSRILEVLTREHGRLSLFARA
                     VRGPKARLAAVLQPFQLLLLSWSGRGEAAQLTAAEAAAHDPPLPPASLMASFYLNELL
                     LKLTTRHDPLPAVFDDYHAAVESLRHGTSVEPVLRVFEKRLLDSLGYGPDLSGEAHTG
                     KPIEAGTYYHFRPALGLVPAASEAGGALAGSSLIRLAGEQLSDAQELEDARRLLLAAL
                     THCLEGRELNTRSVARSIRGLGPRAGSGSRLSARKSRD"
     gene            1415..2140
                     /gene="pdxJ"
                     /locus_tag="VFB37_09990"
     CDS             1415..2140
                     /gene="pdxJ"
                     /locus_tag="VFB37_09990"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZO22820.1"
                     /translation="MTIALGFNIDHIATLRQVRRAHYPDPVHAALAAEMAGADSITLH
                     LREDRRHIQPEDVHALKDRLQTHMNLELAVTEEMLGIAAQVSPADCCFVPERRQELTT
                     EGGLDVAGQVARLKEATARMRAAGVRVALFIDPDPHQIDAAAQTGAPAIELHTGAYAD
                     SRGARRASELERLRAGARQAAALGLEVHGGHGLHYNNVQPVAAIADIVELNIGHAIVA
                     RALFAGLPQAVRDMKALMVAARS"
     gene            2137..2520
                     /gene="acpS"
                     /locus_tag="VFB37_09995"
     CDS             2137..2520
                     /gene="acpS"
                     /locus_tag="VFB37_09995"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZO22821.1"
                     /translation="MIFGIGVDVLEMKRVHETLERFGERFTNHLLMPQEREQLARTNR
                     PERFIAMRFAAKEAIVKAMGTGFAHGVWIRDVGVVQNAWGKPEVIFSERGERVRRGLG
                     VGEGHVTLTDEAGLVVAVAVLLKAG"
     gene            2527..3303
                     /locus_tag="VFB37_10000"
     CDS             2527..3303
                     /locus_tag="VFB37_10000"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZO22822.1"
                     /translation="MNTLVFDIETVPDVALGRRLHGLQGLSDEQVAKAMFGLRRQATG
                     NDFLAHEQHRIVAISCLLRSREDLRLWSLGGPGAPEAELIERFFDGIERFCPDLVSWN
                     GGGFDLPVLHYRGLLAGVQAPRYWETGDGDVAFRFNNYLGRYHRRHTDLMDVLSGYQS
                     RARASLANIACLLGLPGKMGFSGAQVWAAFQAGDLDGIRRYCETDVLNTYLVYLRFEL
                     MRASFTRERYAEEVERVKGLLRLSREAHLGQFLAAWETPA"
     gene            3300..>3867
                     /locus_tag="VFB37_10005"
     CDS             3300..>3867
                     /locus_tag="VFB37_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22823.1"
                     /translation="MSSRSRHVASDVEVGRVEALTHEGEGVVREGKTAFVIGALPEER
                     VRFRRTHRHRQHDQAQLIEVLEPSPARVSPRCAHFGTCGGCALQHLAPDAQLEAKQTE
                     LRDSFERLGQVSPKSWLPPLRGPVWSYRRRARLGARFVTKKGRVVVGFRERLAPYVAD
                     VQRCEVLAPPVGELISPLAAMLNRLSIRE"
BASE COUNT          622 a         1209 c         1350 g          686 t
ORIGIN      
        1 aagcactgaa atggaccgag gccgatcagc aggtcttgca gcggattgtg cgctcgggcc
       61 tgccggccat cgccgcggtg aacaaggtgg accgcgtgcg gccgcgggcc cggctgctgc
      121 cctatctcga gcagctctcc gcacgtcaca cgtttgtaga gctcgtgccg ctttcggcgc
      181 tcaagactga caatgtggat gagctgcgcc gtgtgatcgc cgcccatctg ccggtggcgc
      241 agcccatgtt cgcccaggac gagctgaccg atcgcagcct cgaattccgc attgccgaga
      301 tgatccgcga gaaactcacc ctggagctca atcaggaagt cccttacggc gtcgccgtcg
      361 aggtcgaaca gctccgtgag caggacgagc agctcatggt ggatgcggcg atctgggtgg
      421 accgggaggg tcagaagccg atcgtgatcg gcgcgggggg cgagcggctg aaacgtgtcg
      481 gccgctcggc gcgcctcgcg ctcaacgggt tgctcgggcg gcgcctgcat ctgaacctgt
      541 gggtgaaagt gcgcgagaat tgggcggaca atgcccgtgc actgaagcaa ctcggcctcg
      601 aatgacccgc tccgcccgtc gcatcacgct cgctcccgcc tacatcctgc atcacaggcc
      661 ctatcgcgac accagccgga ttctggaggt gctgacgcgc gagcacggcc gtctgtcgct
      721 gtttgcgcgc gccgtgcggg gcccgaaggc gcgactcgcg gccgtactgc agccttttca
      781 gctgctgctg ctgtcctgga gcgggcgagg ggaggccgct cagctcaccg ccgccgaagc
      841 ggcggcccat gatccgccgc tgccgccggc ctctctcatg gcgtcgttct atctgaatga
      901 gctgctgctg aagctcacta cgcggcacga tccgctgccg gctgtgttcg atgactatca
      961 cgcggcggtc gagagtctgc gtcacggcac ctcggtcgag ccggttctgc gggtgttcga
     1021 gaagcgcctg ctggattcgc tcggttatgg acccgatctg agcggcgaag cccacacagg
     1081 caagcccatc gaggcgggca cgtactatca tttccggccg gcgctggggc tggtgccggc
     1141 ggcctcagag gcgggtgggg ctcttgcagg gtcttcgctg atccgcctcg ccggcgagca
     1201 gctctcggat gcgcaggagc tggaggatgc gcggcggctg ctgctggctg cgctcacaca
     1261 ctgcctggag ggccgcgagc tcaacacccg ctccgtggcg cgctccatcc gcggcctcgg
     1321 gccacgggcg ggctctggat cgcgcctcag tgcgcgcaaa tcccgggatt gacatgccgc
     1381 acccatgcag tggatggcgg aggagtgggc cagaatgacg atcgctctcg gattcaacat
     1441 cgatcacatc gcgacgctgc ggcaggtacg gcgggcgcat tatcccgatc cggtgcatgc
     1501 cgccctggct gcggaaatgg ctggagccga cagcatcacc ctgcatctgc gtgaggatcg
     1561 ccggcatatc caacccgaag atgtgcatgc tctgaaggat cgacttcaga cacacatgaa
     1621 tctcgagctg gcggtaaccg aggagatgct cgggatcgcc gcgcaggtca gtccggccga
     1681 ctgctgcttc gtgcccgagc gaaggcagga gctcaccacg gaaggtggct tggatgtcgc
     1741 cggtcaggtg gcacgcctga aggaggcgac cgcccggatg agggcagcgg gcgtgcgcgt
     1801 ggcgctgttc atcgacccgg acccgcatca gatcgatgcg gcagcgcaga ctggcgcgcc
     1861 ggcgatcgag ctgcacaccg gggcctacgc cgatagccgc ggtgcgcgcc gggcgagcga
     1921 gcttgaacgt ctgcgcgccg gggcgcggca ggctgcagcg cttggcctgg aggtgcatgg
     1981 cggacatggc ctgcattaca acaacgtcca acctgtggcc gcgattgccg acatcgtcga
     2041 gctgaacatc gggcacgcca tcgtggctcg cgccctgttc gccggcctgc cgcaagcggt
     2101 gcgggacatg aaggctctca tggtggccgc acgctcatga tcttcggcat cggcgtcgat
     2161 gtgctggaga tgaagcgcgt gcacgaaacg ctcgaacgct tcggcgagcg cttcacgaac
     2221 catctgttga tgccacagga acgcgagcag ctcgccagga ccaaccgccc ggagcgattc
     2281 atcgccatgc gcttcgcggc caaggaagcc atcgtgaaag cgatgggaac aggcttcgct
     2341 cacggggttt ggattcgcga tgtcggagta gtccagaacg cctggggcaa gccggaagtg
     2401 atcttttccg aacgcggcga gcgcgtccgc cgtggactgg gcgtggggga aggccacgta
     2461 accttgacgg atgaggcggg gctcgtcgtc gcggtggccg tgctgctcaa ggcaggctga
     2521 gaccccatga atacgttggt gttcgatatc gagacggtgc cggatgtcgc tctcggccgc
     2581 cggctgcatg gcctgcaggg cctgagcgat gagcaggtgg cgaaggcgat gtttggcctg
     2641 cgccgccagg ccacgggtaa cgatttcctc gcccatgagc agcatcggat cgtggcgatc
     2701 tcctgcctgt tgcgcagccg cgaggacctg cgcctgtgga gcctgggggg cccgggcgct
     2761 cccgaggcgg agctgatcga gcgcttcttc gacggcatcg agcgtttctg tccggacctc
     2821 gtttcctgga acggcggcgg cttcgatctg cccgtgctgc actaccgcgg gttgctcgcg
     2881 ggggtccagg cgccgcgcta ctgggaaacc ggagatggcg atgtggcgtt tcgcttcaac
     2941 aactatctcg gacgctacca ccgccgccat accgacctga tggatgtcct ctccggctac
     3001 cagagccgcg cacgcgcttc gctcgccaac attgcgtgct tgctcgggtt gccgggaaag
     3061 atggggttct cgggtgcgca ggtctgggct gccttccagg cgggggacct cgatgggatc
     3121 cgccgatact gcgagaccga tgttctcaac acgtatctgg tttacctgcg ttttgagctc
     3181 atgcgcgcga gcttcacgcg cgagcgttac gccgaggagg tggagcgcgt caaggggctg
     3241 ttgaggctct ccagagaggc ccatctggga caatttcttg cggcctggga gacccctgct
     3301 tgagctcgcg ttcgcgtcat gtggcctctg atgtcgaggt cggaagggtg gaggcgctca
     3361 cgcacgaagg tgaaggggtc gtgcgcgagg gcaaaaccgc ctttgtcatc ggcgcgcttc
     3421 ccgaggagcg tgtgcgcttc cgccgaacac atcgccatcg acagcacgat caggcgcagc
     3481 tgatcgaggt gctcgaaccc tcacccgcac gggtgtcgcc gcgctgcgca cactttggta
     3541 cttgtggcgg ctgtgctctt cagcacctgg cgccggatgc gcagctcgag gcgaaacaga
     3601 ccgagctgcg tgacagcttc gagcggctgg gccaggtgag cccgaaatcc tggctgccgc
     3661 ccctgcgcgg gccggtctgg agctatcggc ggcgggcacg gctgggtgca cggttcgtca
     3721 cgaagaaagg ccgcgtggtc gttggatttc gtgagcggct tgctccgtac gtggcggacg
     3781 tgcagcgctg tgaagtgctg gcaccgccag tcggggagtt gatctcgccg ctcgcggcga
     3841 tgctgaatcg cctgagcatt cgcgagc
//
LOCUS       DASRHN010000251         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_908576, whole genome shotgun sequence.
ACCESSION   DASRHN010000251 DASRHN010000000
VERSION     DASRHN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908576"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFB37_10010"
     CDS             <1..274
                     /locus_tag="VFB37_10010"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZO22824.1"
                     /translation="LYGGPAGIVRPFDHWNRARLLVQGMHVEHWLNGQKVVEYELQSP
                     DWKRKVAASKFAAYPNYGLATTGLIGLQGDHPGALAIRHIRIRELP"
     gene            289..909
                     /locus_tag="VFB37_10015"
     CDS             289..909
                     /locus_tag="VFB37_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HZO22825.1"
                     /translation="MAGPTLRSADLFPTRVWQAHLDALIPHFDQWVLHVMKMRAASPS
                     PAGRTNRQGWNSKDMAVLEQAEFAPLRNAIRAACESALGEMGVRGADFELQSWINLHD
                     RGGFNFLHLHEGSLLSGSFYLKVPPGSGEFVFRDPRPGVIHGSVKGSGPNGHCDISLT
                     PSAGLVVLFPCWMEHYVEPHDADEPRITIAFNANERRGGNSERRER"
     gene            complement(889..>2509)
                     /locus_tag="VFB37_10020"
     CDS             complement(889..>2509)
                     /locus_tag="VFB37_10020"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO22826.1"
                     /translation="TLGELLLGSGRSSEAETLLQRAATGPRAYPYAALLLARYYNDTG
                     RPTDALTIAEPLCLAGKADAELASQHIAALVALGRPQDAVGAYQALAANSPGNPASAH
                     ALAYALTATGQYEEAQRVAQHTLARGYRSAPLYSLYARSLIAQGSLDRAEGLLRECLQ
                     IEPRLVEAHNNLAQLIWLRTGELSQAIEMLDGALQRFPNDDALWAAKAALLQGAGNPG
                     AAYDCLAPLAARTQASPMILVRTGLAALESNAATAAALAERALKLLPGSAPARKLLVA
                     ARLGTGDAQGALTECDILLASSPNDQYLIALQSTAWRLLGDERYRQLCDYEKLVMPMQ
                     LEVPSGWADMASFLKDVQVSLSRLHNPAGHALLFQSLRHGTETTQDLSRSPDPAIRAL
                     FNSFVTPIERYIEHMGSGPDPLRRRRAGGWRFNGSWSVRLQPSGFHTNHVHPRGWISS
                     ACYIDLPDCITDTRTQEGILTFGEPSIPTNPALWAEYSVRPAAGMLVLFPSYFWHGTL
                     PFSGTQTRLTVAFDAVPDRERDAAASALTTL"
BASE COUNT          443 a          785 c          838 g          443 t
ORIGIN      
        1 gctttacggc ggccctgccg gcatcgtgcg gcccttcgac cattggaacc gcgcgcggct
       61 cctcgtccag gggatgcacg tggagcactg gctcaacggc caaaaggtcg tggaatacga
      121 gctgcagagc cccgactgga agcgcaaagt ggcggcgagc aagttcgccg cctatccgaa
      181 ctatggtctg gcgaccacgg gcctgatcgg cctgcagggc gatcacccgg gtgctctggc
      241 gattcgccac atccgcatac gcgagctgcc gtaggaattg cggtccccat ggccgggccc
      301 accttgcgct ccgctgacct ctttccgacg agagtctggc aggcgcacct ggacgcgctg
      361 atccctcatt ttgaccagtg ggttctgcac gtgatgaaga tgcgcgccgc gagcccctcg
      421 cccgcgggcc gaaccaatcg ccagggctgg aacagcaagg atatggcggt cctggagcaa
      481 gcggagttcg cgcccctgcg caatgcgatc cgcgcggcat gtgaaagtgc actgggtgag
      541 atgggggtgc gtggagccga ttttgagctg cagtcctgga tcaacctgca tgatcgaggc
      601 ggattcaact tcctgcatct gcacgaggga tcgctcctgt ccggcagctt ctacctcaag
      661 gtgccgcccg gatccggaga gttcgttttc cgggatccgc ggcccggcgt cattcacggc
      721 tccgtcaagg gctcgggccc aaacggacat tgcgacatct ctctcactcc gagtgccggc
      781 ctggtcgtgc tctttccctg ctggatggag cactacgtcg agccacatga cgcagatgag
      841 cctcgcatca cgatcgcttt caacgcgaac gagcggcgcg gcggcaattc agagcgtcgt
      901 gagcgctgag gcagccgcgt ctcgctccct gtcgggcacc gcatcgaatg ctaccgtcag
      961 tcgggtctga gtgccggaga aagggagggt gccgtgccag aaatatgagg gaaacagcac
     1021 cagcattccg gccgctggtc gcacggagta ctcggcccat agagccggat tcgtgggaat
     1081 actcggctcg ccgaatgtca gaatgccttc ctgggttcgc gtatcggtta tgcaatccgg
     1141 caagtctatg tagcaggcgg aggagatcca gccccgcgga tgcacatggt tggtatggaa
     1201 gcccgaaggt tgcagacgca cggaccagct gccattgaag cgccagccgc cagccctgcg
     1261 gcgacgcaga ggatcgggtc ccgagcccat gtgttcgatg tagcgctcga tcggcgtcac
     1321 gaaactgttg aagagcgccc ggatcgccgg atccgggctg cgggacagat cctgagtggt
     1381 ctcggttccg tggcgcagag actgaaacaa cagcgcgtgg cctgcggggt tgtgcaagcg
     1441 actcaggctg acctggacgt ccttcaggaa gctcgccatg tcagcccagc cagagggcac
     1501 ctcgagctgc atgggcatga ccagtttctc gtagtcacag agttgcctgt agcgctcatc
     1561 gcccagcaga cgccaggcag tgctctgcag ggcgatgaga tattgatcgt ttggagagct
     1621 cgccaggagg atgtcgcact ccgtcagggc accctgggca tctcccgtgc cgaggcgcgc
     1681 tgcgaccagc agctttcgcg caggcgcact tcccggtaag agcttgaggg ctcgctctgc
     1741 gagtgccgcc gccgtggcgg cgttgctctc cagggccgca agtcctgtgc ggaccaggat
     1801 catgggagag gcttgtgtgc gtgccgccag cggagcgagg caatcatagg cagcacccgg
     1861 gttccccgca ccctgaagaa gggcagcctt ggccgcccag agcgcatcgt cattcgggaa
     1921 cctctgcaac gccccatcga gcatctcaat ggcctgcgat agctccccgg tgcgcaacca
     1981 gatcagttgc gcgaggttgt tgtgcgcttc gacgagccgc ggctcgatct gcaggcactc
     2041 ccgcagcaga ccttcggcac gatcgaggga tccctgggca atcagactcc gggcgtagag
     2101 gctgtacagc ggggccgagc ggtatccgcg tgcaagggtg tgctgcgcga ctcgctgcgc
     2161 ttcttcgtat tgcccggtcg cagtcagtgc gtaagccagc gcatgggcgc tagccggatt
     2221 gccgggtgag tttgcggcga gggcctggta agccccgact gcgtcctggg gacgccctaa
     2281 tgccacgagc gcggcgatgt gctgggaggc caactccgca tccgccttcc cggcaaggca
     2341 caggggctcg gctatcgtca gcgcatccgt cggacggccc gtgtcattgt agtaacgggc
     2401 cagaagcaag gcggcatacg gataggcacg gggcccggtg gccgcgcgct gcaacagcgt
     2461 ttcggcctcg ctgctacggc cgctgccgag cagcagctcg cccagggtg
//
LOCUS       DASRHN010000252         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_908791, whole genome shotgun sequence.
ACCESSION   DASRHN010000252 DASRHN010000000
VERSION     DASRHN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908791"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1409)
                     /locus_tag="VFB37_10025"
     CDS             complement(24..1409)
                     /locus_tag="VFB37_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO22827.1"
                     /translation="MASQQTVSPVDGSVYCERALADSDHIEAVLSRAVHAQRAWRSVP
                     IAERATICNRFCEHFEAQRAPLALELTWQMGRPARQSPGEISGTLERARYMISVAAEA
                     LAEINPGPKPGFERFVRREPLGLVFTIAAWNYPYLIAVNSVIPALMAGNSVVLKHSAQ
                     TPLCAERFAEGLRAAGLPPGVFDYLHLSHEDTERVVADPRVAFVAFTGSVEGGRAIQR
                     VAAAGFAGVGLELGGCDPAYVRQDANIPHAIESLVDGAFFNSGQSCCGIQRIYVHERV
                     YQQFVAGAIELAGKYVLGVPTDPDTTLGPVVRVAAAERIRREVGACLASGARSGLDAR
                     RFPASRPGTPYLAPELLLDVDHTMSVMREEIFGPVAGVMRVRSDEEAVRLMNDSRFGL
                     TAAVWTQDAEAALSIGERIQTGTWFVNRCDYLDPALAWTGVKDSGRGCTLSRVGYEQL
                     TRPKSFHLRQL"
     gene            complement(1430..2815)
                     /locus_tag="VFB37_10030"
     CDS             complement(1430..2815)
                     /locus_tag="VFB37_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22828.1"
                     /translation="MSTQTLLNPRQVRTVADARAIVAERELTHVKVGVFDNDGILRGK
                     YMARPKFESALESGFGFCDVVLGWDSRDQLYDNARYTGWHTGYPDAPVRLIPESCRAL
                     PWEEPVLFFLGEFSGAARKVCPRGLLQQVLERARAMGYEALAGFEYEFFVFSETPDSV
                     REKHYRGLKPIAPGNFGYSVIRNSVWSDFYRSLLETCERMDFPLESLHEETGPGVIEA
                     AIAADGALAAADKAALFKTFAKVLAQRQGLMATFMAKWSMDWPGQSGHIHLSLRNTAG
                     KPVFHDSNGSHGMSDTMRWFVGGQQRLMPELLAMIAPTVNSYTRLIPGFWAPTDSAWS
                     VDNRTCALRVIRGSPKSQRVEYRVAAADANPYVSLSAAIGSGLWGIEHRIEPEPMVEG
                     NAYERSHPPELQLPRTLWEAAQRLKGSSMARECFGEEYIEHFAASREWEEREFRRHVT
                     DWELARYFEII"
     gene            complement(2897..>3246)
                     /locus_tag="VFB37_10035"
     CDS             complement(2897..>3246)
                     /locus_tag="VFB37_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22829.1"
                     /translation="AQVYPPQSGDPKETPGSTVERDLSSGKIDAAVVWGPFAGLMVSE
                     HSGPHAWTAVPFKADQKIKFDYEISMGVRFGEKKWKDTLDQWISSHQAQINSILTSYH
                     IPLLPTTEAAPAS"
BASE COUNT          590 a         1108 c          982 g          566 t
ORIGIN      
        1 gttccatggc ggatgtgtgc gggtcacagc tgacgcaagt gaaaggattt gggccgtgtg
       61 agctgctcgt atcctacacg tgagagcgtg cagccccgcc cggaatcctt cacaccggtc
      121 caggcgagcg ccgggtcaag atagtcgcat cgattcacga accacgtgcc cgtctgaatg
      181 cgctcgccga tggaaagtgc ggcctcggca tcctgggtcc acactgcggc ggtcagaccg
      241 aatctgctgt cattcatcaa tcgcacggcc tcctcgtcgc tgcgcacacg catgacgccc
      301 gccaccggtc cgaagatctc ctcgcgcatg acagacatcg tgtggtccac atcgagcagc
      361 agctccggag caagatatgg cgtacccgga cgacttgcag gaaaccgccg cgcatcgagc
      421 ccactgcgcg ctccgctcgc gaggcaagcc ccgacttccc gacgaatccg ctccgccgcc
      481 gcaacccgca ccaccggccc gagcgtcgtg tccgggtcgg tcggaacgcc cagcacgtac
      541 ttgcctgcca actcgatcgc gcccgccacg aactgctggt agacgcgctc gtgcacatag
      601 atgcgctgga tcccgcagca ggactggcca ctgttgaaga acgccccatc cacgaggctc
      661 tcgatcgcgt gcggtatgtt ggcatcctgg cgcacatagg ctggatcgca acctcccaat
      721 tcgaggccga cacccgcgaa gccggctgct gccactcgct gaatagcacg gccgccctcg
      781 accgagccgg tgaaggccac gaaggccaca cgcggatcgg ctacgacccg ttcggtgtcc
      841 tcatggctca ggtgcagata atcgaacacc cccggcggca gaccggcggc gcgcaagcct
      901 tccgcaaagc gctcggcgca caaaggggtc tgcgctgagt gtttcaacac gaccgagttg
      961 ccggccatca gcgccggaat gacgctgttg acggcgatca ggtacggata attccaggcc
     1021 gcgatcgtga acacgaggcc gagcggctcg cggcggacga atcgctcgaa tcccggcttc
     1081 ggtcccggat tgatctcggc gagcgcctcc gctgcaacgg agatcatgta gcgggcccgc
     1141 tccagagtcc cactgatctc cccggggctc tggcgagccg ggcggcccat ctgccaggtc
     1201 agctcgagcg ccaagggcgc gcgctgcgcc tcgaagtgct cacagaagcg attgcagatt
     1261 gtcgcccgct cggcgatggg tacgctgcgc catgcgcgct gcgcgtggac cgcgcgactg
     1321 agcacggcct ctatgtgatc agagtctgcg agcgctctct cgcagtacac cgagccatcc
     1381 accggactga cggtctgttg cgaagccatg agactctggc gccgggctct cagatgatct
     1441 cgaagtaacg cgcgagctcc cagtcggtca catgccggcg gaattcgcgc tcctcccatt
     1501 cacgactcgc cgcaaagtgc tcgatgtact cctcgccaaa gcactcccgc gccatggagg
     1561 agcccttgag gcgctgcgcg gcctcccaca gcgtgcgcgg gagctgcagc tccggcggat
     1621 ggctgcgctc ataggcattg ccctcgacca tgggctcggg ctcgatccgg tgctcgatgc
     1681 cccacagccc tgagccaatg gccgcagaca gactgacata cgggttcgcg tccgcggcgg
     1741 cgacgcgata ctcgacacgc tgcgatttcg gacttccccg gatgacccgc agcgcgcacg
     1801 tccgattgtc gacgctccag gcagaatctg tcggtgccca gaatcccgga atcaatctcg
     1861 tgtaggaatt gacggtgggt gcaatcatcg ccagcagctc cggcatcagt ctctgctggc
     1921 cgcccacgaa ccaacgcatc gtgtcgctca tgccgtgcga cccgttggaa tcgtggaata
     1981 caggtttgcc cgcggtgttg cgcagggaaa ggtgaatgtg cccgctctgg ccaggccagt
     2041 ccatggacca tttggccatg aacgtggcca tcaacccttg cctctgtgcg agcaccttgg
     2101 cgaaggtctt gaagagagcg gccttgtcgg cagcggcgag cgccccgtcc gctgcgatag
     2161 ccgcctcgat cacaccgggg ccggtctcct catgcaggct ttcgagcgga aaatccattc
     2221 tctcgcaggt ctcgaggagc gatctgtaga agtcgctcca gacactattg cggatcacgc
     2281 tgtagccgaa gttgccgggc gcgatcggct tcagtccccg gtaatgcttc tcgcgtaccg
     2341 agtcgggcgt ctcgctgaac acgaagaact cgtattcgaa tccagcgagg gcctcgtaac
     2401 ccatcgcccg ggctcgctcc aacacctgct gcagaagacc acgcggacag accttccggg
     2461 cggcgccgct gaactccccc aggaagaaca aaaccggctc ctcccacggc agcgcccggc
     2521 agctctcggg tatcagccgc accggcgcat cggggtaacc ggtatgccag cccgtgtagc
     2581 gcgcattgtc atagagctga tcgcggctat cccagcccag cacgacgtcg cagaagccga
     2641 aaccgctctc gagtgccgac tcgaacttgg gtcgcgccat gtatttgccg cgcagaatcc
     2701 cgtcattgtc gaacacacct accttgacat gcgtgagctc gcgctcggcc acgatcgcgc
     2761 gcgcatcggc gacagtccgc acctgccgcg gattcaggag ggtctgtgtg gacattcccc
     2821 tatgccatgg agatccatga ctgccagtat agacagtgcc ggcccggggg cgcgaccgcg
     2881 ctgcccgcgc agttgctcag ctcgccggcg cggcctcggt cgtcggcaac agcgggatgt
     2941 gatagctcgt caggatggag ttgatctgcg cttgatgcga gctgatccac tgatcgagcg
     3001 tgtccttcca cttcttctca ccgaaacgca cgcccatcga aatctcatag tcgaacttga
     3061 tcttttgatc cgccttgaag gggaccgccg tccaggcgtg tggcccgctg tgctcgctca
     3121 ccatgagtcc tgcgaacgga ccccacacga cggccgcatc gatcttgccc gagctcagat
     3181 cgcgctcgac ggtgctgccc ggagtctcct tggggtcacc gctctggggc gggtagacct
     3241 gcgccc
//
LOCUS       DASRHN010000253         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_751569, whole genome shotgun sequence.
ACCESSION   DASRHN010000253 DASRHN010000000
VERSION     DASRHN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751569"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1181..1654
                     /locus_tag="VFB37_10040"
     CDS             1181..1654
                     /locus_tag="VFB37_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO22830.1"
                     /translation="MTEIYDLQSYEPRKGVGYLIHRVKAEMLAIVDRELAEDEQLNAL
                     EVSSAQFIILSTLAQGGQKSASDLCKGISYDAGAMTRMIDRLEDKGLLQRTRCPQDRR
                     LVYLELTDAGRAAFPRMREISLRVLNRFLRGFSKAEARQLESFLLRMLENAPASE"
     gene            1781..3376
                     /locus_tag="VFB37_10045"
     CDS             1781..3376
                     /locus_tag="VFB37_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO22831.1"
                     /translation="MNAKVMQWMTRGIAGLRELGSYAAVELLVPGGSVLALSLYFYRR
                     YAKRHPASHLLLIRRAGLAAAAIATTLLAACASTHGLAPHSTEQKADNLTASQVLAGT
                     NVADTAWPQEGWWRQFGDPQLNELITEGLAGSPSLRVAEARTRAALGQAGIAGAARFP
                     QINAQADATRERFAQHSIYPPPFAGNWDTMSELQASLSWELDLWGKNRSAYEQALGNA
                     RATQLDAQAARLILASNIAHAYVQLERAYLQLDVAQATLKQREQIYKLTQDRNEAGVD
                     SKLELRQAESALPAAREQIAQLEESIELTRDQLAALLGKGPDRGLAITRPAANALTTV
                     SLPSRIPSELLGRRPDILADRLRIEAARHGIDNARAQFYPDVNLSAFVGFQNLGPTAL
                     VSAANREVGAGPAISLPIFDAGRRRGNLAVRDSEYDIAVEQYNQALADAMREVVDQLA
                     SFKSIDQQRHEQREGLSTAQDAYDLATMRYREGVGNYLQVLTTETQLLAQRSLDADLR
                     ARSLDLSINLARALGGGFQKLEQ"
     gene            3378..4736
                     /locus_tag="VFB37_10050"
     CDS             3378..4736
                     /locus_tag="VFB37_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO22832.1"
                     /translation="MNAQNDTQKVSNSSPAAAPVSPAATAPASTATAPARNRRLLWLS
                     ILSGAFLLIGAGYGAYWATVLRYRQTTDDAYVNGNVVQITPQISGTVVAIGADDTQFV
                     KSGQPLVRLDQADAKVALDQAEAQLARTVREVRNLFATSAQLRAAVQLRQSDLDMARK
                     DLERRERLGNSGAISAEELTHARDAYASAQAGLLAAQQQLAGNQARVDGTTVEDHPDV
                     RNAAAAVRSAYLEYSRTVLPAPVSGFVARRNVQLGQRVNPGAPLMSVVPLDQVWVDAN
                     FKEPQLAKMRVGQPVELTADLYGRSTVYHGKVEGFGAGTGSAFALLPAQNATGNWIKI
                     VQRVPVRIALDPREMAAHPLQIGLSMKAEVSVRQDNGARLPELAHTAPAYSTDVFQSP
                     DAMADSRVRAIIAENESAALAKPSDKVATTKSRVGDAAGARSGEATDPDLLAQVRRQR
                     GH"
     gene            4770..>5855
                     /locus_tag="VFB37_10055"
     CDS             4770..>5855
                     /locus_tag="VFB37_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO22833.1"
                     /translation="MAETSNPTAQAAGRPQELQPLTGATLAIGAIALSLATFMNVLDT
                     SIANVSIPAIAGDLGVSPDQGTWVITSFGVANAISLPLTGWLTRRFGQVRLFLWSVGL
                     FTLASFLCALAPTLGMLILFRVLQGAVAGPMMPLSQALLLSSYPKARAGAALALWGIT
                     TLVAPVVGPVLGGWITDNIAWPWIFYINVPVGIVAGLTTWFIYQKRESPTARIPIDKI
                     GLALLVLWVGCLQVLLDKGKDLDWFNSGQIVTLAVVSVVGFVFFLVWELTEEHPVVDL
                     SLLGRRNFLVSTIAMLLAYGLYFGNVVLLPLWLQQYMGYTATLAGLVLAPVGLLAIFL
                     TPVIGRYVNRTDPRVLVTVAFAVFALVL"
BASE COUNT         1028 a         1851 c         1915 g         1061 t
ORIGIN      
        1 ggaccggaac agagcaccag ctcaccgacc tgcctgcagg cttcgtcatc ggcgactttg
       61 acgttacacc agacggcggc gagatcgtct tcgaccgtat tcaggaaagc tcgagcgtcg
      121 tgcttatcga gcgtgcgagg caatcggagt aactctggat cgcccggtcg aacgcggcga
      181 ggagctcccg gtgcagattg gcaaggacga gatgtcgagt gtcccagggc agctgctgtc
      241 tgtgtcttac ttctacccgt gggccgctgg ggattacgct catccattgt gcatcgctgc
      301 gcgaagggtg actggtcatc tgatgcgaga tgcagcagca tctgatgtat gcgcaccaca
      361 ttggaaattg aggacgacgt gctgcaagcc gcgcgagaac gggcgcggta cgagcgaaag
      421 acgatcggag aggtcagcat gcgtccgagg gttgggcctc atgcccgctc actcaaaacg
      481 gctgcgtgcg catcatgtca agcccgggtt acgcgaatcc atttccggtt caggctatcg
      541 ttgggcgctt atcggaggcc tgctcagtgg atgtccacga gttctggtct gacgagttga
      601 gcttgcttga ttccggtgcc ttcgatccaa cacgtatcca cgggccgcgg cacctcacgg
      661 atatatatct gctcggtctg gccgtgcacc acaaagggcg gctagtcacg ttcgattccg
      721 gtatccctct tgcagccgtc cgaggcgcgc atccccggca tcttctggtg ctctagacct
      781 gacccctccg cgccctggca gcggtccgag gaggaccact ggcgaaagat cctgaagcag
      841 gtgagggcag ggcgcactct gcggccgcga gtgtggccgg tcggcatgcg gaccccctcg
      901 tgggacttca gtgcatctac gctcgtcaat cgcctgcaaa ttgggactgc gctccgactc
      961 ctctttgatg gcagatgagg attgttacga gcggctgctg gaaggcgagc ctaggtctca
     1021 tcgagctgcc cgtgcagtgg ggccagaata gtcacgccaa ggcccgagca ggggtcaagc
     1081 cgcatgccca ggcaaaggaa gccttgacaa tgacagccta ggcaattaat atcctggcca
     1141 tccgatgagg agccttccac ggcgcctccc gggaggaagc atgaccgaga tctatgacct
     1201 gcagagctac gagccccgga aaggcgtcgg gtacctgatc caccgggtga aggctgagat
     1261 gctggccatc gtcgatcggg agctcgccga ggacgagcag ttgaatgcgc tggaggtcag
     1321 ctctgcccaa ttcatcatcc tgtcgaccct cgcgcagggt gggcagaagt cggcatccga
     1381 tctatgcaag ggcatctcgt atgacgccgg cgccatgacc cgaatgatag atcggctcga
     1441 ggacaaagga ctgctacaaa ggacgcgttg cccgcaggac cgacggctgg tctatctcga
     1501 gctgaccgat gcgggcaggg cggcctttcc gcgcatgcga gagatttccc tgcgagtact
     1561 caaccgtttc ctgcggggat tcagcaaggc cgaggcgcgt cagctcgagt cctttctgct
     1621 gcggatgctg gagaacgcac ccgccagcga gtgagttttt ttgactatat gtttgcccag
     1681 gcagtgttag ccgcggcagt aagaagtgga tgagaccgcg cgcgggcgcg gagccggagc
     1741 agctctcgaa gcggttccgg tgcaaagagg agactccgcc gtgaatgcga aagtgatgca
     1801 gtggatgact cggggcatcg cgggattacg cgagctgggc tcctacgcgg cggttgagct
     1861 tctggtgccc ggcggctcgg tgctcgctct gagcctctac ttctatcgcc gctacgcgaa
     1921 gcgccatccg gccagccatt tgttactgat ccgtcgcgct ggacttgcgg cggcggcgat
     1981 cgcgacaact ctcctggcgg cctgtgcgag cactcacggg ctcgcgccgc attcgactga
     2041 gcagaaagct gacaacctga ccgcttcaca ggtcctcgcc ggcaccaacg tcgctgacac
     2101 ggcgtggccg caggagggct ggtggaggca gttcggcgat ccgcagctga acgagttgat
     2161 caccgaaggc ctggcaggca gcccctcgct gcgtgtggcg gaagcgcgaa cccgcgctgc
     2221 actcggccag gccggcatcg caggcgctgc acgcttcccg cagatcaatg cacaggccga
     2281 cgcgacacgc gagcgcttcg cccagcactc gatctatccg ccgccgttcg ccggcaactg
     2341 ggacacgatg agcgagctcc aggcgtcgct ttcctgggag ctcgacctgt ggggaaagaa
     2401 ccggtcagcg tacgagcagg ccctcggcaa cgcgcgcgcc acacagcttg atgcgcaggc
     2461 agcacggctg atcctcgctt caaacatcgc gcatgcctat gtgcagctcg agcgcgctta
     2521 tctgcagctc gatgtcgcgc aggcgaccct caagcagcgt gagcagatct acaagctgac
     2581 acaggaccgc aatgaggctg gagtcgactc caagctcgag ctgcgtcagg ccgagtcggc
     2641 gttgccggcg gcgcgggagc agatcgccca gctcgaggag agcatcgagt tgacccgcga
     2701 tcagcttgcg gcgctcctcg gcaagggtcc cgatcgcggg ctggccatca cgaggccggc
     2761 ggcgaatgcg ctgaccacgg tctcgctgcc aagccgcatt ccttccgagc tgctcggtcg
     2821 ccgcccggac atcctcgcgg atcggctgcg catcgaggcc gcccgccacg ggatcgacaa
     2881 tgcccgcgcg cagttctatc ccgacgtcaa tctcagcgcc ttcgtcggct ttcagaacct
     2941 gggtcccacc gcactggtga gcgccgccaa tcgcgaggtc ggcgcgggcc cggcgatcag
     3001 cctgccgata ttcgatgcgg gcaggcggcg cggcaatctc gcagtgcgcg attccgaata
     3061 tgacatcgcc gtggagcagt acaaccaggc gctcgccgat gcaatgcgcg aggtcgtgga
     3121 tcaactggcc tcattcaaga gcatcgatca gcagcggcac gagcagcgcg aaggcctctc
     3181 gacggcgcag gacgcctacg acctggcgac gatgcgctac cgggaagggg tcggcaatta
     3241 cctgcaagtg ttgacgacgg agacgcagct cctggcgcag cgcagtctcg atgcggactt
     3301 gcgcgcccgc tcgctcgatc tatccatcaa tctggcccgc gccctgggcg ggggctttca
     3361 gaagctggag cagtagtatg aacgctcaga acgataccca gaaggtctcg aactcctctc
     3421 ccgcagcggc ccccgtgagc cctgcggcaa cggcgcccgc gagcaccgcc acagcgcctg
     3481 cccgcaatcg tcgcctgctt tggctgtcga tcctgagcgg tgctttcctg ctcatcggcg
     3541 cagggtacgg cgcgtattgg gcaacggtgc tgcgttatcg gcagacgaca gacgatgcgt
     3601 atgtcaatgg caacgtcgtg caaattaccc cgcagatctc gggcaccgtc gtcgcgatcg
     3661 gcgcggacga tactcagttc gtgaagagtg gacagccgct cgtgcggctt gaccaggccg
     3721 atgccaaagt ggcgctcgat caggccgaag cccagctcgc gcgcaccgtg cgtgaggtgc
     3781 gcaacctgtt tgctacctct gcccagctgc gagcggcagt gcaactgcgc cagagcgatc
     3841 tcgatatggc gcgcaaggat ctcgagcggc gtgagcgtct cggcaactcg ggggcgattt
     3901 cggccgaaga gctgacgcat gcgcgcgatg cctatgccag cgcacaggcc gggttactgg
     3961 ccgcccagca gcagctggcc ggaaatcagg cacgcgtgga tgggaccacc gtggaggatc
     4021 atccggacgt acgcaacgcc gcggccgcgg tacgcagtgc ctatcttgag tacagccgga
     4081 cggtgctgcc ggcaccggtg tcgggcttcg tcgcccgccg caatgtgcag ctcggacagc
     4141 gcgtcaatcc gggggcgcct ctgatgtccg tcgtgccgct cgatcaggta tgggtggatg
     4201 cgaatttcaa agagccgcag cttgcgaaga tgcgcgttgg gcagccggtc gagctgaccg
     4261 ccgacctgta cggccgcagc accgtgtatc acggcaaggt ggagggcttc ggagccggga
     4321 ccggctcggc gttcgccctg ttgcccgcac agaatgccac cggcaactgg atcaagatcg
     4381 tgcagcgcgt gccggtgcga attgctctcg atccccgtga gatggcggcg cacccgctgc
     4441 agatcgggct gtcgatgaag gctgaggtct cagtacggca ggacaacgga gcccgcctgc
     4501 cggagctggc gcacaccgcg ccggcttatt cgacggacgt gttccagtcg ccggatgcga
     4561 tggcggactc gcgtgtgcgg gcgatcatcg cggagaatga gtctgcggcc cttgcgaagc
     4621 cgagcgacaa ggtagccacc acgaagtctc gcgttggcga tgcggccggt gcacgctcgg
     4681 gcgaggcgac ggacccggat ttgctggcgc aggtacgccg gcagcgtggg cattgaggca
     4741 ggcccgccag gcacatcgag gaagctgaca tggctgagac gagcaacccg actgcgcagg
     4801 cagcagggcg tccacaggag ctgcagcctc tgaccggtgc aacgcttgcg atcggcgcca
     4861 tcgcactctc gctcgcgacc ttcatgaacg tgctcgatac gtcgatcgcg aacgtgtcga
     4921 ttcccgcgat tgcgggcgac cttggcgtct cgcccgatca gggcacgtgg gtcatcacat
     4981 ccttcggcgt cgcgaatgcc atctcgctgc cgctcaccgg ctggctcacc cgaaggttcg
     5041 gccaggtcag gctcttcctg tggtcagtcg gcctgttcac cctggcgtcc tttctgtgcg
     5101 cgctcgcgcc caccctcggc atgctgatcc tctttcgcgt gctgcagggt gcggtggcag
     5161 gccccatgat gccgctgtcc caggccctgc tgctgtcgag ctatccgaaa gctcgagccg
     5221 gggctgccct cgcgctatgg ggcatcacga ctctggtcgc accggtcgtg gggccggtgc
     5281 tcgggggctg gatcaccgat aacatcgcgt ggccctggat cttctacatc aacgtgccgg
     5341 tgggtatcgt cgcgggcctc accacctggt tcatctacca gaagcgcgaa agcccgaccg
     5401 ctcgcatccc gatcgacaag atcggcctgg cgctgctcgt attgtgggtc ggatgcctgc
     5461 aggtcctgct cgacaagggc aaggacctcg actggttcaa ctccggacag atcgttaccc
     5521 tcgcggtcgt ctcggtcgtc ggctttgtct tcttcctggt gtgggagctg accgaagagc
     5581 acccggtggt ggatctgagt ctgctcgggc gacgcaactt cctcgtcagc acgatcgcca
     5641 tgctgctcgc atatgggctg tacttcggca acgtcgtgct gctgccgctg tggctgcagc
     5701 agtacatggg ctacacggcg acacttgccg gcctggttct cgcccccgtc ggactgctgg
     5761 cgatcttcct gactccggtc atcggccgct atgtgaatcg caccgatccc agggtgttgg
     5821 tgaccgtcgc cttcgccgtt ttcgcgctgg tcctg
//
LOCUS       DASRHN010000254        13497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1901543, whole genome shotgun sequence.
ACCESSION   DASRHN010000254 DASRHN010000000
VERSION     DASRHN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13497
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901543"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1276
                     /locus_tag="VFB37_10060"
     CDS             143..1276
                     /locus_tag="VFB37_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZO22834.1"
                     /translation="MKPWLHPLCCLLTLLAVPAGATVYDLPDNGTAVFGSDERIKSHF
                     QDTLLDIARRYSLGYEEIIRANPGVDMWLPGEGTDILLPGRRILPPGPRQGIVVNLPE
                     HRLYYFPKPKKGDKPVVITFPVSIGKMDWHTPLGETHVIAKLKHPIWYPPESVRKEHE
                     ANGDPLPKLVPAGPDNPLGDFAMRLAVGDGTYLIHGTNNPMAVGMAITHGCIRMYPED
                     VAALFPLVPVGTPVWLINDPVKVAYVDGELLLEAHPPVDAEGQTLEPNLDLLSQLLDK
                     ALGNDTAAIHWDLARQTLQAATGMPTVVGLQAYLDQPGGPAPTAGASALSADKVPGAT
                     LPNATTSTATAPAATEAPAATKDGGANSGPTSVAAAAAPPNNH"
     gene            complement(1310..1648)
                     /locus_tag="VFB37_10065"
     CDS             complement(1310..1648)
                     /locus_tag="VFB37_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22835.1"
                     /translation="MNRLQDLEARRQELLLRCEAQRLELAYHLAQAGPAGRWNALKRR
                     IGPQSVASHPLAWIASIIGLLMMMRTRKVLSGVTWIGGLIALASRATTLLRLFAQIRT
                     LLQNVKAQRR"
     gene            complement(1645..2049)
                     /locus_tag="VFB37_10070"
     CDS             complement(1645..2049)
                     /locus_tag="VFB37_10070"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZO22836.1"
                     /translation="MTDASATEDVTEQEHPTGVLRNLIASLIAAGRTRLDLAAVETEI
                     FLLRTVRMLLWAVAAVACALLALAFAVVALVAALWDTHRMAGVLGGTALFIILAIIFG
                     IVASRSLRQHPQLLEGTLAQLQNDQHQAGGSP"
     gene            complement(2046..2336)
                     /locus_tag="VFB37_10075"
     CDS             complement(2046..2336)
                     /locus_tag="VFB37_10075"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HZO22837.1"
                     /translation="MTDLNVGKLADDLQTLVSDAEELLRATAETAGDRTSEARDRAEE
                     SLRAVRSRLASVERDLRGRARVVGDYVEDNPWKAIAMAGGVALILGLLMGRK"
     gene            complement(2358..3491)
                     /locus_tag="VFB37_10080"
     CDS             complement(2358..3491)
                     /locus_tag="VFB37_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22838.1"
                     /translation="MIVVTVNSGSTSVKLAAYVSENAGAWSRIAQEPHAGSAAQPVTV
                     LQEFVGKLEGCVGAVAHRVVHGGTRFRGPALLDAQAMKAIDQLSELAPLHNPKALHWI
                     EAARQVVGEQVPQVAAFDTSFFCDMPAVAGEYALAPRFGVDQAVRRYGFHGLAHEALW
                     RRWCELHPELRRGGKLITLQLGGGCSAAAIAGGQPLDTTMGFSPLEGLVMATRSGDVD
                     PAVVPYLQHRLSRTAEEIIELFNRESGLSGVAGGETDLGTLAADPSPRSQFALDLYCY
                     RIRKCLGAFFAVLGGCDGIVFGGGVGEHVPAVRSRVLSGMAWAGVRIAEEANQAARGQ
                     DASIDAPESSVRVHVLAVDEEGVLVRAVRTILDGHAACHSERT"
     gene            3560..5971
                     /locus_tag="VFB37_10085"
     CDS             3560..5971
                     /locus_tag="VFB37_10085"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZO22839.1"
                     /translation="MPAKPNADQAPAPLSAAQYQALEAWWRATNYLAVGQIYLLANPL
                     LREPLSLEHVKPRLLGHWGTTPGINFIYAHLNRIIIARERDVLAIIGPGHGGPALAAN
                     TWLEGSYSELYPDISQDLQGMQRLFKQFSFPGGIPSHCAPELPGSINEGGELGYSLAH
                     AFGAAFDNPDLLVACIIGDGEAETGPLATAWHSNKFLNPARDGAVLPILHLNGYKIAN
                     PTVLARIPHDELESLLKGYGWEPFFVEGDEPKPMHELMASTLDRVLDRIDRIQREARS
                     QRSVSRPRWPMIVLRSPKGWTGPKVVDGQQVEGTWRAHQVPLAGLATQPQHLRMLEEW
                     LRSYRPAELFDEQGAPVPLLRELCPAGDRRMGSNPHANGGLLLRDLDLPDIGKHAVRI
                     TRPGAEQAESTRVLGGYLREVFERNAAANNFRLWGPDETSSNRLDEVFEATQRTWMAQ
                     LLPGDTHLSPDGRVMEVLSEHQCQGWLEGYLLSGRHGLFNCYEAFIHIVDSMFNQHAK
                     WLESSHRIPWRRAVSSLNILLSSHVWRQDHNGFSHQDPGFIDVVCNKKSAVVRVYLPP
                     DANTLLCVVDQCLRSRDLVNVIIAGKQPEPQWLDIDAAFKHCAAGIGMFDWAGNDDPG
                     EPDVVMACAGDVPTMEVLAAVSILRQHAPELRIRVVNVVDLMTLQPAEEHPHGLKHSE
                     FDALFTTDKPILFAFHGYPWLIHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMVVRNDL
                     DRFHLVMDVIDRVPSLGARAAHLRQLMRGKLTEHAHYITQRGEDLPEVREWVWQDRRD
                     ESMNR"
     gene            6014..7126
                     /gene="tal"
                     /locus_tag="VFB37_10090"
     CDS             6014..7126
                     /gene="tal"
                     /locus_tag="VFB37_10090"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZO22840.1"
                     /translation="MPTHNPLQELRKLGQSIWLDDIGRRMLSDGSLARLISEDGIAGV
                     TSNPSILAHSITGDPQYAKAITELLPKVSSSMALYEALAMEDLRGAADLFRPLYDSLQ
                     GKDGFVSLEVSPHLAHDTAGSIAEAKRLWSRLDRVNAFIKIPGTVAGIEAIRQLITEG
                     VNVNVTLLFAPERYKRVAEAYCDGLEARIAAKKPIDRLASVASFFLSRIDSNVDPQLD
                     RLAAAGQPAARALRGKAAIANACRAYQLYTELVASPRWEKLASAGARTQRLLWASTST
                     KDPTYSPIKYVEELIAPDTVNTAPKETIDAYRQRGQPQPRLERHLADAQDVRDGLERL
                     GIDLEQVAEQLEAEGVRKFIEPFDKLQGWLEERRSR"
     gene            7237..7584
                     /locus_tag="VFB37_10095"
     CDS             7237..7584
                     /locus_tag="VFB37_10095"
                     /inference="COORDINATES: protein motif:HMM:NF045308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZO22841.1"
                     /translation="MDIEAVRERLLGRREELRHRASQASADLRHESDPLSADFAEQAT
                     QRENDDVLGAISQSARSELGLLEAALRRLAQGRYATCALCGEPIDESRLRAVPYTDRC
                     CACAEGSLKPSRR"
     gene            complement(7586..8851)
                     /gene="glgC"
                     /locus_tag="VFB37_10100"
     CDS             complement(7586..8851)
                     /gene="glgC"
                     /locus_tag="VFB37_10100"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZO22842.1"
                     /translation="MNSNVPTPHQQAPDTLAIVLAGGNGTRLGELTRWQCKPAIPFAG
                     HFRNIDFTLSNCINSGLRRVAVLTQYKAQTLISHLTGGWSFLPRALGEFVEIWPAQQR
                     LHSSWYAGTADAVHQNLDMVLAQRTRYTLVLAGDHIYKMDYRPLIEQHAHSGADVTLA
                     CVPVPLEEAASFGVLGVEGGQRVRSFIEKPQPASLRATNQRTVLASMGVYVFCTDYLA
                     DRLRRDANNPDSAHDFGRNILPCAVREDHVGAYSFTDAKGGAAYWRDVGTLDAYWQAH
                     MELLVAAPPIELFDPAWPIFTSAEQLPPARVMADAMSEACVIDSLLGGGVIVRGARVS
                     RSVLAANVQVGAGSTIAEAVILPNARIGANCRLQRVIVDAGMQIPDGTVAGVAPTAEL
                     QSPRSGGVTLLVQEPAESAHPDDGLRSVA"
     gene            complement(8885..9829)
                     /locus_tag="VFB37_10105"
     CDS             complement(8885..9829)
                     /locus_tag="VFB37_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZO22843.1"
                     /translation="MSSAYDRLPLNALRVFEAVASRLNFGEAAEALHVTPAAVSQQIR
                     SLEDYLQTPLFRRDGRRVQLTPEGVQLLPGVRQGLDELEAALQQIRQERQKGPINVSM
                     LASFLQKWLMPRLGALHARHPDIELRLHTSPAAVDFARTDFHAALRFGLGRYGDLFTE
                     KFLGEWLVAVAAPKVLKKHGPLPNQGDLSPYPLLHGDELDWANWSTGNTRSMSALRGS
                     FIDDSVSLMSAVVEGLGFGLLRWTLAASEIEAGRLALASEHIVRHRFSYYFVCPAAYA
                     SFPKVAALREWLLQQARSFAPPPEPREASTAGRTRSKT"
     gene            9903..10721
                     /locus_tag="VFB37_10110"
     CDS             9903..10721
                     /locus_tag="VFB37_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZO22844.1"
                     /translation="MAATFKASGVITITTDFGHQGPFVGVMKGCILTRFPAARIIDLT
                     HEIVVHWPAEAGFWLARAFPYFPAGTVHVAVIDPGVGTSRDIVAVAAQEQLFLAPDNG
                     LLAPIVSRHADAQVVRFDAPKLARFGVQRPSATFHGRDIFAPIAAELAAGRCQPAELG
                     PPAGNLVPAWVDEPTVEAGSIAGVVITIDHFGNLITNIDGALVGRFRQPLVQAGNHTF
                     PLLRTYGDTQPGEFLALVNSFGVLEIARAEQSAAEGLGLSRGAPVLVREGTRRN"
     gene            10839..11054
                     /locus_tag="VFB37_10115"
     CDS             10839..11054
                     /locus_tag="VFB37_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22845.1"
                     /translation="MSERDNGSFDLDDEFLNGPTEDGTDYDRLLDRVDKRARNQGKRG
                     KAAWSRLEEVLADKKLEKDLLDFDEEL"
     gene            11126..>13497
                     /locus_tag="VFB37_10120"
     CDS             11126..>13497
                     /locus_tag="VFB37_10120"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HZO22846.1"
                     /translation="MTRVASSWIVLKFGGTSVSSLSNWRNIAAVLRARLAGGARVLLV
                     HSAVTGITDRLDKLLEAALVRTQDSELRGIEERHARLAAELGVPVGTAVEQSLAELRQ
                     IAAGIALVGEVSDHTRARVLATGELMATELGAAFLRTLGLQVDWVDARSLLKAEERRG
                     AHARSGVLSATCNFEPDALLEARLGALAPVVITQGFIASDADGNTVLLGRGGSDTSAA
                     YLAAKLRAQRLEIWTDVPGMFSANPRSTPTARLLRALHYDEAQEIATSGAKVLHPRCI
                     LPVRQHGIPLHVYATQAPELEGTVLSAEGGDAGAQVKAVCTKKGITLISLESPGMWHQ
                     VGFLADAFQVFKQHGMSVDLVSTSETNVTVSLDPAANTLDNSLLNALVADLSRLCRVR
                     AIGPCASVSLVGRNIRAILHQLGDAFEFFEEQKIYLVSQAANDLNFTFVVDENQGDRL
                     VEQLHELLIRPVPGDLVLGPTWEQLFLEPKSGGSRTLPWWRHKRAELLGALGERQCAF
                     VYDVQTIHAAARALRGMEALTRVHYSMKANSSAPVLRAVHSQGVDFECVSRGELERAL
                     GLFPELNPQRILFTPNFAPREEYAWAFERGVRVTVDNLYSLTAWPDLFAKQDIFLRID
                     TGVGRGHHHHVRTAGAHSKFGVPIAELKQVGLHAQRIGARVMGLQAHLGSGIFDVTSW
                     QHTARRLADIAQQFADVQVINVGGGLGVPERGDQPGVDLQKLDTLLAAVRAEHPRLEF
                     WMEPGRYLVASAGVLLARVTQLKSKGEVRYVGVATGMNSLLRPALYGAYH"
BASE COUNT         2410 a         4427 c         4361 g         2299 t
ORIGIN      
        1 aggatcccgc agggcgcgaa gtcgcgggcc ggttttcgag ccgcgcgtgg cacttttgac
       61 tagaatgctc gtggcagccc ccgaaattgg ggcatactat gttcgggaat tcagaacgtt
      121 acggattgtg caaagaattc acatgaagcc ttggctccat cccctctgtt gtctgctcac
      181 cctcctggcc gtgcccgcag gggcgacggt gtacgacttg cctgacaacg gcacggccgt
      241 gttcggcagc gatgagcgca tcaagtccca cttccaggac accctcctgg atatcgcgcg
      301 ccgctatagc ctgggatacg aggagatcat tcgcgcgaac ccaggcgtgg atatgtggct
      361 gccgggggag gggaccgaca ttctgctgcc cggccggcgc atccttccgc cagggccccg
      421 ccaggggatc gtcgtcaatt tgccagagca tcgcctgtac tactttccca agccgaagaa
      481 aggtgacaag cctgtcgtca tcacctttcc ggtgagtatc ggcaagatgg actggcacac
      541 gccgctgggc gagacgcacg tcatcgcgaa gctgaagcac cccatctggt atccgccgga
      601 gtccgtgcgc aaagaacacg aggcgaacgg cgatccgttg cccaagctcg tgcccgcggg
      661 gccagacaat ccgctcgggg atttcgccat gcggctcgcg gttggtgatg gcacctacct
      721 cattcacgga accaacaacc ccatggccgt tggaatggcc attacacatg gctgcatccg
      781 catgtatccg gaggacgtgg cggccttatt tccgctcgtg ccggtgggca ctccggtctg
      841 gctgatcaac gatccagtga aggtcgcata tgtggacggt gagctgctgc tcgaagccca
      901 tcctcctgtg gacgccgaag gtcagactct ggaacccaac ctcgatctgc tgtcgcagct
      961 gctggacaag gcgctcggca acgataccgc cgcgattcac tgggatctgg cgcgccagac
     1021 attacaggct gccacgggga tgccaacggt ggtgggtttg caggcctatc tcgatcagcc
     1081 cggcgggccc gcgccgacgg ccggtgcgag tgctctgagt gccgacaagg tgccgggtgc
     1141 gactcttccg aacgccacta catcaactgc gacggcgccg gctgcgaccg aggcacctgc
     1201 tgcaaccaag gacggtggtg ccaattcggg gccgacgtcg gttgccgcgg cagccgcgcc
     1261 acccaacaat cattgatccc caggcgtcgc gcaggtccta tcgagtcgat cagcgacgct
     1321 gtgcttttac gttctgaagc agcgtcctga tctgggcgaa caggcgtagc agggttgtgg
     1381 cgcgagaggc caaggcaatc aggccgccga tccaggtgac gccgctcaac accttgcggg
     1441 tccgcatcat catcagcaac ccgatgatgc tcgcgatcca ggcgagagga tggctggcga
     1501 cggactgcgg cccgatgcgc cgtttgaggg cgttccaccg cccagcggga ccggcttgcg
     1561 ccaggtgata agccagctcg aggcgctgtg cttcgcagcg caacagcagt tcctgacgcc
     1621 gcgcttccag atcctgcagg cggttcacgg cgagcctccc gcctgatgct gatcgttctg
     1681 gagctgtgcg agagtgcctt cgagcagctg gggatgctgc cgcaggctac gagaggcaac
     1741 gatgccgaag atgatggcca ggatgatgaa caacgcggtg ccgcccagca caccggccat
     1801 ccgatgcgta tcccacagag cggccaccag cgcgaccacg gcgaaggcga gcgccagcag
     1861 tgcgcaagcc actgccgcaa ccgcccacag gagcatccgc acggtacgca gcaggaagat
     1921 ctccgtctcc acggccgcaa ggtccaggcg cgtcctgccc gccgcgatga ggctggcgat
     1981 caggttacga aggacccccg tcggatgttc ctgttccgtc acgtcctcgg tggcggacgc
     2041 atcggtcatt tgcgtcccat cagcagtccc aggatcagtg caacgccacc ggccatcgcg
     2101 atggccttcc atggattgtc ctcgacgtaa tcgccgacga cgcgcgcccg gcctcgcagg
     2161 tcgcgctcca ccgaggcgag ccggctgcga accgcacgta acgactcttc ggctcgatcg
     2221 cgagcttccg aagtccggtc accggcggtc tccgccgtgg cgcgcagcag ttcctcggca
     2281 tcgctcacca gggtctgcag gtcatccgcc agtttgccaa cgttcaaatc ggtcatttcg
     2341 gcactcctgt tgatgggtta cgttcgttcg gagtggcaag cagcgtgccc gtccagaatg
     2401 gtgcgtaccg cccggaccag taccccctcc tcatcgacgg caagcacgtg caccctgaca
     2461 gagctctccg gtgcatcgat gctcgcgtcc tggccgcggg cagcctggtt ggcctcctct
     2521 gcaatccgga cacccgccca ggccatgccg gagagcaccc ggctgcggac cgccggaacg
     2581 tgctcgccga ccccgccacc gaagacgata ccatcgcagc cccccaggac ggcaaaaaag
     2641 gcgcccaggc acttgcggat ccggtagcaa tacaaatcga gcgcaaactg cgaccgggga
     2701 gagggatcgg ccgccagcgt gcccagatcg gtttcacctc cagcgacacc gctgagaccc
     2761 gattcccggt tgaagagctc gatgatttcc tcggcggtcc gcgaaaggcg gtgctgcaga
     2821 tagggaacga ctgccggatc gacgtcaccg ctgcgcgtgg ccatgaccag gccctccaga
     2881 ggggagaagc ccatggtcgt atccaggggc tgacccccgg caatcgccgc ggccgagcaa
     2941 ccgcctccga gttgcagcgt gatgagcttt ccgccccgcc gcaactcggg gtgcagctcg
     3001 caccagcggc gccagagggc ctcgtgcgca aggccgtgga acccataacg ccgcacagct
     3061 tgatcgacac cgaagcgggg ggccaaggcg tattcgccgg cgacggcggg catgtcgcag
     3121 aagaacgagg tgtcgaacgc ggccacctga ggaacctgtt ccccgacaac ctgccgggca
     3181 gcctcgatcc agtgcagggc cttggggttg tgcagaggtg cgagctccga gagttgatcg
     3241 atcgccttca tggcttgcgc atcgagcagc gcgggacctc tgaaccgagt gccgccgtgg
     3301 accactcggt gcgcgaccgc cccgacgcaa ccttcgagct tccccacgaa ctcctgcagc
     3361 accgtaacag gttgcgctgc gctgccggca tgcggctctt gggcaatccg gctccatgca
     3421 cccgcgttct cgctgacgta ggcagccagc ttgaccgagg tgctgccact attgactgtc
     3481 acgacaatca tcgacactca ccggaatgtt taatgctggg tgacccggtt ctacccagca
     3541 aggtgatgag gaccttcgca tgcccgccaa acctaacgca gaccaggctc cggcgccgct
     3601 ctccgccgcg cagtatcagg ctctggaagc ctggtggaga gcgacgaact acctcgccgt
     3661 gggccagatc tatctgctcg ccaatccgct gctgcgcgag cccctgtccc tcgagcacgt
     3721 caagccgcgc ctccttgggc actggggcac gacgccgggc atcaacttca tctacgcgca
     3781 cctgaaccgc atcatcatcg ctcgcgagcg cgatgtcctc gcaatcatcg gtcccggtca
     3841 cggtggcccg gcgctcgccg ccaacacctg gctcgagggc agctacagcg agctctatcc
     3901 tgacatcagc caggacctcc aggggatgca gcggctgttc aagcagttct cctttccggg
     3961 cgggattccg agccactgtg cacctgagct gcccggctcg atcaatgagg gcggcgagct
     4021 cggatactct ctggcgcacg cgttcggggc cgccttcgac aatcccgacc tgctcgtcgc
     4081 ctgcatcatt ggcgatggcg aagcggaaac cggcccgctc gccaccgcct ggcattccaa
     4141 caaattcctg aatccggcac gcgacggggc cgtgctgccg atcctgcatc tgaatggtta
     4201 caagatcgcg aaccccacgg tgctggcgcg cattccgcat gacgagctcg agagcctgct
     4261 caagggctat ggctgggagc cgttcttcgt ggagggtgac gagcccaagc ccatgcatga
     4321 gctcatggcc tccacactcg atcgggtgct cgatcgaatc gaccggattc agcgcgaggc
     4381 gcgctcgcag cgcagcgtga gccggccacg ctggccaatg atcgtgctgc gcagtccgaa
     4441 gggctggacc ggccccaagg tggtggacgg gcagcaggtc gaaggcacct ggcgggcgca
     4501 ccaggtgccg ctggccgggc tcgccacgca gccgcaacac ctgcgcatgc tcgaggaatg
     4561 gctgcgcagc tatcggccgg cggagctctt cgacgagcag ggcgcacccg tgccgctgct
     4621 gcgggagctg tgtcccgcag gcgaccggcg catgggctcg aatccgcacg ccaacggtgg
     4681 actgctgctc cgcgacctgg atctaccgga tatcggcaag cacgctgtca gaatcacccg
     4741 gcctggtgcg gaacaggccg agtccacgcg cgtgctgggc ggttacctgc gcgaggtttt
     4801 cgagcgcaat gcggcggcga ataatttccg cctgtggggg ccggatgaga ccagctccaa
     4861 tcgcctggac gaggtcttcg aagccacgca acgcacctgg atggcgcaac tgctgccggg
     4921 cgacacgcac ctcagccccg acggacgcgt aatggaggtg ctctccgagc atcaatgtca
     4981 gggctggctg gagggctatc tgctttcggg ccgccacggt ctgttcaact gctacgaggc
     5041 tttcatccac atcgtggact cgatgttcaa ccagcatgcg aagtggctcg agtcctcgca
     5101 ccgcatcccg tggcggcgcg ccgtgtcctc gctcaacatc ctgctgagct cccatgtatg
     5161 gcgacaggac cataacggat tctctcatca ggacccgggg ttcatcgatg tcgtgtgcaa
     5221 caagaaatcc gcggtcgtgc gtgtttatct gccccccgat gccaacaccc tgctgtgcgt
     5281 cgtggatcaa tgcctgcgca gccgggacct ggtgaacgtg atcatcgccg gcaagcagcc
     5341 ggagccgcag tggctggaca tcgatgcggc cttcaagcac tgcgccgccg gcatcggcat
     5401 gttcgactgg gccggcaacg atgatcccgg ggagccggat gtcgtcatgg cgtgcgccgg
     5461 tgatgtgccc acgatggagg tgctggctgc cgtcagcatt ctgcgccagc acgccccgga
     5521 gcttcggata cgggttgtca atgtcgtgga tctgatgacc ctgcagcccg cggaggaaca
     5581 tccgcacggt ctgaagcaca gcgagttcga tgcgctgttc accaccgaca agcccatcct
     5641 gtttgctttc cacggctatc cgtggctcat ccaccgtttg acctaccgcc gcacgaatca
     5701 tgacaacctg catgtgcgcg gctacaagga agagggcact accaccacgc ccttcgacat
     5761 ggtggtccgc aacgacctgg accgcttcca cctggtcatg gatgtgatcg accgggtgcc
     5821 gagcctcggc gctcgggccg cgcatctgcg tcagctcatg cgtggcaagc tcaccgagca
     5881 tgcgcactac atcacgcagc gcggcgagga tcttccggag gtgcgggagt gggtctggca
     5941 ggatcgccgc gatgaaagca tgaaccgatg atgagcgtac aatcgcatcc gaagaccatc
     6001 gcaggtgatc gccgtgccca ctcataaccc gctgcaggaa ctccgcaagc tcggccagag
     6061 catctggctg gatgacatcg gccgacggat gctgtccgac ggcagcctgg cccggctcat
     6121 ctccgaggac ggcatcgcag gggtcacctc gaatccatcg atcctcgccc actcgatcac
     6181 cggcgatccg cagtacgcca aggcgatcac ggagctgctg cccaaggtct cgagcagcat
     6241 ggctctgtac gaggcgctcg cgatggagga tctgcgcggc gcggcggacc tgttcaggcc
     6301 gctgtacgac tcactgcagg gcaaggatgg tttcgtgagc ctggaagtat ccccgcatct
     6361 ggcgcatgac acggcgggaa gcatcgctga ggccaagcgg ctgtggagcc ggctggatcg
     6421 cgtcaacgct ttcatcaaga ttcccggcac cgtcgccggc atcgaggcga ttcgccagct
     6481 gatcaccgaa ggcgtcaacg tcaacgtgac gctgttgttt gcgccggagc gctacaagcg
     6541 ggtggccgag gcctattgcg atggtcttga agcgcggatc gcggcgaaga agccgatcga
     6601 ccgccttgct tcggtagcca gcttctttct cagccgcatc gacagcaacg tggatccgca
     6661 gctcgatcgg ctggccgcgg ccggtcaacc tgccgcccgt gcgctgcgtg gcaaggctgc
     6721 cattgccaat gcgtgccggg cctatcaact gtacacagaa ctcgtggctt ccccccgctg
     6781 ggagaagctg gccagcgcgg gcgcccggac gcagcggctg ctctgggctt cgacctcgac
     6841 caaggacccg acctacagcc caatcaaata tgtggaggag ctgatcgctc cggacacggt
     6901 gaacaccgcg cccaaagaga cgatcgatgc gtaccgccag cgtggccaac cgcagccgag
     6961 gcttgagcgg caccttgcgg atgcccagga cgtgcgggac gggctcgagc ggctcgggat
     7021 cgacctcgag caggtcgctg agcagctcga ggccgagggc gtacgcaagt tcatcgagcc
     7081 cttcgacaag ctgcagggct ggctggagga gcgacgctcg cgctagtgcg cgtgccaagt
     7141 tggttgcctg tccgagccta gttccccagc ctgcggattt ggtgcacgct atcgccggag
     7201 tccgctatcc ggacggcgac cagggggaag cgccacgtgg atatcgaagc ggtcagagag
     7261 cgtctgctcg gcaggcgcga ggagctgcgc catcgggcga gccaggcgag cgccgatctg
     7321 cgccatgaat ccgatcccct gagtgccgac ttcgccgagc aggcgacgca acgcgagaac
     7381 gacgatgttc tcggggcaat ctcccaatcc gcaaggtccg agttgggcct gctcgaggcc
     7441 gcattgcgcc gtctcgcgca aggtcgctac gccacctgcg cgctctgtgg tgaaccgatc
     7501 gatgaaagtc ggctccgggc ggtgccctac accgatcgat gttgtgcgtg tgcggaaggc
     7561 tcgctgaagc cctcgcggcg ctgattcagg cgaccgatcg cagcccatca tcagggtggg
     7621 cactctcggc gggttcctgc accagcaggg tgacgccgcc ggagcgaggg gactgcaatt
     7681 ccgcagtcgg cgcaaccccg gccaccgtgc cgtccgggat ctgcatcccg gcgtcgacga
     7741 tgacccgctg cagtcggcaa ttcgcgccga tgcgtgcatt gggcaggatg acggcctcag
     7801 cgatcgtcga gcccgccccg acctggacgt tggccgccag aacactgcga ctgacgcggg
     7861 caccccggac gatcacacca ccgccgagca gagaatcgat cacgcaggct tcggacatgg
     7921 catcggccat gactcgcgcg gggggcagct gctctgccga ggtaaagatt ggccacgcag
     7981 ggtcgaacag ctcgatgggg ggtgccgcga cgagcagttc catgtgggcc tgccagtacg
     8041 catcgagtgt cccgacatcg cgccagtatg cggcgccgcc tttggcgtcg gtgaaggagt
     8101 aggcgcctac atggtcctcg cgtacggcgc acggcaggat gttgcggcca aagtcatgtg
     8161 ccgagtccgg gttgttggca tcacggcgca ggcgatcggc gaggtaatcg gtgcagaaga
     8221 cgtaaacccc catcgaggcg agcacggtcc gctgattcgt cgcgcgcaga ctcgcaggtt
     8281 gaggtttctc gataaagctt cgaacgcgct gtccgccctc cacgccgagg accccgaagg
     8341 aggccgcctc ctcgagcggt accggcacac aggccagggt cacatcggca cccgagtggg
     8401 cgtgctgctc gatcagcggc cgatagtcca tcttgtaaat gtgatcaccc gccagcacca
     8461 gtgtgtaacg ggtacgctgc gccagcacca tatcgagatt ctgatgcacg gcatccgcgg
     8521 tgcctgcgta ccaggaggaa tgcagccgct gctgtgccgg ccagatttcc acgaactcac
     8581 cgagcgcccg cggcagaaag ctccatccac ccgtaagatg cgagatgagc gtctgcgctt
     8641 tgtactgcgt gagcactgca actcggcgca gaccggagtt gatgcagttg gagagcgtga
     8701 agtcgatgtt gcggaagtgg ccggcaaagg gaatcgccgg tttgcattgc cagcgggtca
     8761 actcgcccag ccgtgtgcca ttgccacctg ccagtacgat tgccagggtg tccggtgcct
     8821 gctggtgcgg cgttggaacg ttcgaattca cgctcccgcc cggaccgcaa gaaccgtacc
     8881 tattctaggt cttactgcgg gtgcgacccg cggtcgaagc ctcgcgtggt tccggcggcg
     8941 gcgcgaagga acgtgcctgc tgcagcaacc actcgcgcag cgccgcgacc ttggggaagc
     9001 tcgcgtacgc tgcgggacac acgaagtagt aggagaatcg atgccgcacg atgtgctcgc
     9061 tcgccagtgc gagacggccg gcttcgatct cgctcgccgc aagcgtccag cgaagcaagc
     9121 cgaagcccag tccctccacc accgcactca tcaggctcac cgagtcatcg atgaaggagc
     9181 ccctgagcgc gctcatcgag cgggtgttgc ccgtggacca gtttgcccaa tcgagctcat
     9241 cgccgtgcag cagcggatag ggtgacagat cgccttggtt cggcaaaggc ccgtgcttct
     9301 tcagcacctt cggggctgcg acggccacca gccactcgcc caaaaatttc tcggtgaaca
     9361 gatccccgta gcgtccgagt ccaaagcgca gcgcggcatg aaagtccgtg cgcgcgaaat
     9421 ccaccgcagc cggcgaagta tgcaggcgca gctcgatatc gggatgccgg gcatgcagag
     9481 cgccgagccg cggcatgagc cacttttgca ggaaggatgc cagcatgctc acgttgatcg
     9541 ggcccttctg gcgctcctgc cggatctgtt gcagcgccgc ctcgagctcg tccagacctt
     9601 gccgcacgcc cggcagcagc tgcaccccct cgggagtcag ctgcacccgc cgcccgtcgc
     9661 gcctgaaaag cggcgtctgc agataatcct cgagcgagcg gatctgctgg ctcacggccg
     9721 ccggcgtcac atgcagagcc tccgcggcct cgccgaaatt gagccggctc gccaccgcct
     9781 cgaacacacg cagggcgttg agcggcagcc ggtcataagc ggagctcacg gtaagaccac
     9841 cccgggaaat cgcgcgctgc agttgacagg ctcacgggat gcggacatgg ttttcctctg
     9901 caatggctgc caccttcaaa gcctcgggcg tcatcaccat caccaccgac ttcggacatc
     9961 agggtccgtt cgtcggcgtg atgaaggggt gcattctcac ccgttttccc gcagcccgga
    10021 tcatcgatct gacgcacgag atcgtcgtac actggcccgc cgaagcgggc ttctggctgg
    10081 cgcgggcctt tccttacttc ccagcaggga ccgtacacgt ggcggtcatc gatccggggg
    10141 tcggcacctc cagggacatc gtggcggttg cggcgcagga gcagctcttt ctggcacccg
    10201 acaatgggtt gctcgccccg atcgtgtccc gtcatgccga tgcacaggtg gtgcgcttcg
    10261 atgcgccgaa acttgcgcgc ttcggcgtgc agcgccccag cgcgaccttt catgggcggg
    10321 acattttcgc gcccatcgcc gcggagctgg cagccggccg ctgccagccc gccgagctcg
    10381 gtccgccggc ggggaacctc gtgccggcgt gggtcgatga gcccacggtg gaagccggca
    10441 gcatcgccgg tgtggtcatc accatcgatc acttcggcaa tctgatcacc aacatcgacg
    10501 gggcactcgt cggacgcttc cgccaacccc tggtccaggc gggcaatcac acctttccgt
    10561 tgctgcggac ctacggcgat acccagcccg gggagttcct cgcgctcgtc aattccttcg
    10621 gcgtcctcga gatcgcgcgc gccgagcaga gcgcggccga gggcctgggc ttgagccgtg
    10681 gcgcccccgt gctggtgcgc gaggggaccc ggcggaatta ggctcgggcg ccgcgccggg
    10741 gttgatccgc ggcgcgaatc ggatatagtt cgctgccctg cggagcgcgc tgaatgcgct
    10801 ttagctgttg ataaacctga gaatttagtc gttgagccat gtcggaacgc gacaacggga
    10861 gcttcgatct ggacgatgaa ttcctgaacg ggccgaccga ggacggcacc gattacgacc
    10921 gcctgctcga tcgggtggac aaacgcgctc gtaaccaggg caagcgcggc aaggctgcat
    10981 ggagtcgact cgaggaggtt ctggcggaca agaagctcga gaaggacctg ctggacttcg
    11041 acgaagagct ctgaagcgcc gaggctgctc ttcttcatcc ggcaacgagg cggcctgctc
    11101 ctccggccgc ccaagggacc agtcagtgac gcgcgtggct tcctcctgga tcgttctgaa
    11161 gttcggcggc accagcgtct cctcgctctc gaactggcgc aacatcgccg ccgtgcttcg
    11221 cgcccgcctc gcaggcgggg cccgcgtgtt gctcgtgcac tccgcggtca ccgggatcac
    11281 cgacaggctg gataaattgc tggaagccgc gctggtgcgc acgcaggact cggagctgcg
    11341 tgggatcgag gagcggcacg ctcgccttgc ggcggagctc ggtgtaccgg tggggacggc
    11401 cgtcgagcaa tcgctcgcag aactgcggca gattgccgcg ggaatcgccc tggtcggcga
    11461 agtcagcgat cacacacgcg cccgtgtgtt agccaccggc gagttgatgg cgaccgagct
    11521 cggtgcagca ttcctgcgca cgctcggcct gcaggtggac tgggtcgacg cgcgcagctt
    11581 gctcaaagcg gaggaacgtc ggggcgcaca tgcgcgctcg ggcgtgctct ccgcgacatg
    11641 caacttcgag ccagatgccc tcctcgaggc acgactcggc gccctggcac cggtcgtcat
    11701 cacgcaggga ttcatcgcca gtgatgccga cggcaacacc gtgctgctcg gaagaggcgg
    11761 gtcggatacc tcggcggcat acctcgcagc caagctccgc gcgcagcgcc tggagatctg
    11821 gaccgacgtg ccggggatgt tcagcgccaa tccacgctcg acgccgaccg cgcggctgct
    11881 gcgcgctctg cactacgatg aggcccagga aattgccacc agcggggcta aggtcctgca
    11941 cccgcggtgc atccttcccg tgcgccagca tggaatcccg ctgcacgtct atgccaccca
    12001 ggctccggag ctcgagggca ccgttctgag cgcagagggc ggggatgcgg gggcgcaggt
    12061 caaggcggtg tgcaccaaga agggcatcac gctcatatcg ctcgaaagcc cgggaatgtg
    12121 gcaccaggtt ggatttctgg ctgatgcctt ccaggtcttc aagcaacacg gcatgtcggt
    12181 cgatctggtg tccacctcgg aaaccaacgt cacggtatcg ctcgatcccg ccgccaacac
    12241 cctcgacaac tcgctcctga atgcgctggt ggcagatctg tcgcgcctgt gccgggtgcg
    12301 ggcaatcggc ccgtgcgcat ccgtgagcct ggtcggtcgc aacatccgcg ccatcctgca
    12361 tcagctcggc gatgcgttcg agttcttcga ggagcagaag atctacctgg tgagccaggc
    12421 agccaacgat ctgaacttca ctttcgtggt ggacgagaat cagggcgacc ggctggtcga
    12481 gcagctgcac gagctcctca tccgacccgt gcccggcgac ctcgtactcg gcccgacctg
    12541 ggagcagctg ttcctcgagc ccaagagcgg cggctcacgc accctgccct ggtggcgtca
    12601 caagcgagcg gagctgctcg gcgcgctcgg cgagcggcag tgcgcattcg tgtacgacgt
    12661 tcagacgatt cacgccgccg ctcgcgcctt gcgcggcatg gaggcgttga cgcgcgtgca
    12721 ttactccatg aaagcgaact cgagcgcccc ggtgctgcgc gcggtgcatt cgcagggggt
    12781 ggacttcgag tgcgtctcac gcggcgagct cgagcgggcg ctcggattgt ttccggagct
    12841 caacccgcag cgcattctgt tcacgcccaa ctttgccccg cgcgaggagt acgcctgggc
    12901 cttcgagcgg ggtgtgcggg tgacggtgga caacctgtac agcttgacgg cttggccgga
    12961 tctatttgcg aagcaggata tctttctgcg catcgacacc ggtgttggac gcggacacca
    13021 tcatcacgtg cgcacggccg gtgcacattc caagttcggt gtgccgattg cggagctcaa
    13081 gcaggtgggg ctgcacgcgc agcggatcgg agcgcgcgtg atgggcctgc aggcccatct
    13141 gggcagcgga atcttcgatg tcacgagctg gcagcatacg gcccggcgac tcgccgacat
    13201 tgcgcagcaa tttgccgacg tgcaggtgat caacgtgggg ggagggctgg gtgtgcccga
    13261 acgcggcgat cagcctggcg tggacctgca gaagctcgat actctgctgg ctgccgtccg
    13321 tgccgagcat ccgcgactgg aattctggat ggagccgggc cgctatctcg tggcgagcgc
    13381 cggcgtgctg ttggcgcgcg tcacgcaact gaagtcgaag ggcgaagtgc gctacgtggg
    13441 cgtcgcgact gggatgaact ccctgctgcg tccggcgctc tatggggcat accatga
//
LOCUS       DASRHN010000255         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_752503, whole genome shotgun sequence.
ACCESSION   DASRHN010000255 DASRHN010000000
VERSION     DASRHN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752503"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..503)
                     /locus_tag="VFB37_10125"
     CDS             complement(186..503)
                     /locus_tag="VFB37_10125"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZO22847.1"
                     /translation="MSPQDRAQISVRIMEKEYVVACSYEERSALLDAAEFLNGRMREI
                     RDSGKLVGHDRIAVMVALNLAHELLRIKNQDAKLEGEVGNRLRVLRERVEGALEKGQQ
                     LEL"
     gene            complement(500..730)
                     /locus_tag="VFB37_10130"
     CDS             complement(500..730)
                     /locus_tag="VFB37_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HZO22848.1"
                     /translation="MSDTTKTQLDLELMRLTKRIEELIATVEHLKEENRALRQRHESL
                     ATERATLMHKNEQVRTRVEAMIGRLKTLEHGA"
     gene            806..1363
                     /locus_tag="VFB37_10135"
     CDS             806..1363
                     /locus_tag="VFB37_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HZO22849.1"
                     /translation="MHPEHYTDIQRVLAEERSLADAAEAHGTLAGCLCAAIGYRFEDW
                     LLEILPEGQAQPLSRETLRELYMHTAGALEGPEMQFELLLPEDAQPIDARTAALAQWC
                     QGFLYGLGTSVIQDESGLPGDIGEIVRDLTEITHVAVDSRQTEESNESAYAELVEFVR
                     VGVQLVFDELGPLRDQPAPPGVPLH"
     gene            1360..2682
                     /gene="pepP"
                     /locus_tag="VFB37_10140"
     CDS             1360..2682
                     /gene="pepP"
                     /locus_tag="VFB37_10140"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF008131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HZO22850.1"
                     /translation="MKAARPAKDEFARRRRLLRRFMGRDSIAILPSAPVRHRNNDVEY
                     AYRQDSDFYYLTGFDEPEAVAVLIPGREHGEYVMFVRDRDPTRETWDGRRAGPAGARR
                     QYGADDAFPISDIDEILPGLLENRDKVYYTMGVYADFDQRVVGWVNGLRTQARNGRHP
                     PQEFVALDHVLHDMRLFKSRGEIGLMRESARIASAAHVRAMRFCRPGRREYEVMAELM
                     HEFRRHNADISYHPIVGGGPNSCVLHYRENDQPLRDGDMLLVDAGCEYECYASDVTRT
                     YPVNGRFTPEQRAVYEIVLEANRTAIAKVRPGNHWNEPHLAAVHVITQGLVRLGLLKG
                     RVAKLERDGAYRRFFMHRTGHWLGMDVHDVGDYKIGDEWRVFEPGMVLTVEPGIYIPA
                     STRGVPRRLRNVGVRIEDDVVVTRTGAEVLTARAPKDADAIESLMSSG"
BASE COUNT          507 a          894 c          959 g          524 t
ORIGIN      
        1 gggacgaact gcagcaggta aggctttccg ctcgaagcgg acttgcacaa ttctgcccgc
       61 aagtcctgat gtcccggggt gtttcaatta gggtccgagg taacccggcc cgcttgtcga
      121 acaccgcagg cgacgccggg agacattcta ccgggttcgc gtcaggccgc gaatcgagaa
      181 tcgcgtcaca gctcgagctg ctggcccttc tcgagcgcgc cctcgacccg ctcacgcagg
      241 accctcaggc ggttgcccac ctctccctcg agcttcgcgt cctggttctt gatgcgcagc
      301 agctcatggg cgagattgag cgcaaccatg acggcgatgc ggtcgtgacc tacgagcttg
      361 ccgctatccc ggatctcgcg catgcggccg ttgagaaact cggccgcatc cagcagggcc
      421 gagcgctcct cgtacgagca tgcgacgaca tattcctttt ccatgatgcg cacgctgatc
      481 tgcgcgcggt cctgtggact cacgcgccat gctccagcgt cttgaggcgg ccgatcatcg
      541 cctcgacgcg cgtgcggacc tgctcgttct tgtgcatcag cgtggcgcgc tcggtggcga
      601 gggactcgtg tcgctgacgc aacgcgcgat tctcttcctt gagatgctcg acggtcgcga
      661 tcaactcttc gatccgcttg gtgagtcgca tcagctccag atcgagctgc gtttttgttg
      721 tgtcgctcat gctctgacta tagagcgggg ctcttcgcgc ggtcaatctt ggagtcaggt
      781 ccgttgaggc ataatacgtg cagccatgca tccggagcac tacaccgaca tccagcgcgt
      841 cctggccgag gagcgctcgc tggccgatgc cgccgaggcg cacgggaccc tggccggatg
      901 cctgtgcgcg gcgatcggct accgcttcga ggactggctg ctcgagattc tgccggaagg
      961 gcaggcccaa ccgctgtcgc gggagaccct gcgcgagctg tacatgcaca cggcaggggc
     1021 gctcgagggc ccggagatgc agttcgagct gctgctgccc gaggacgcac agcccatcga
     1081 tgcccgcacg gcggccctcg cgcaatggtg ccagggcttc ctgtatggcc tcggaacgag
     1141 tgtcatccag gatgaaagcg ggcttccggg tgatattggt gagatcgtgc gggacctgac
     1201 agagatcact cacgtggcgg tggactcgcg tcagaccgag gagtccaacg agagcgccta
     1261 cgccgagctg gtcgaattcg tgcgtgtggg cgtgcagctc gtgttcgatg agctgggacc
     1321 cctgcgggat cagcccgccc caccgggcgt accgctgcat tgaaagccgc ccgccccgcg
     1381 aaggacgagt tcgcgcgtcg gcggcggctg ctgaggcgat tcatggggcg cgactcgatc
     1441 gccatcctgc cctcggcccc ggtccggcat cgcaacaacg acgtggaata cgcgtaccgg
     1501 caggacagtg acttctacta tctgaccgga ttcgatgagc ccgaagcggt cgccgtgctg
     1561 atccccggcc gcgagcacgg ggaatatgtc atgttcgtca gggatcggga ccccacgcga
     1621 gagacgtggg atgggcgccg ggccggcccc gccggagcgc ggcgccagta cggcgccgac
     1681 gacgcctttc cgatctcgga catcgacgag atcctcccgg ggctactgga gaaccgggac
     1741 aaggtctatt acacgatggg ggtgtatgca gatttcgatc agcgtgtcgt cggctgggtg
     1801 aatggcttgc gtacgcaggc gcgcaatggg cgacacccgc cgcaggagtt cgtcgcgctc
     1861 gatcacgtgc tgcacgacat gaggttgttc aaatcccgcg gcgagatcgg cctgatgcgc
     1921 gagtctgcgc gcatcgcttc tgcggcgcac gtgcgcgcga tgcggttctg caggcctgga
     1981 cggcgcgagt acgaggtgat ggcggagctc atgcacgagt tcaggcgcca caacgcggat
     2041 atttcctatc atccgatcgt cggcggggga cccaatagct gtgtcctgca ttacagggag
     2101 aacgatcagc cgctgcgcga cggcgacatg ctgctcgtgg atgccggctg cgaatacgaa
     2161 tgctatgcct ccgatgtcac acgcacctat cccgtcaacg ggcgattcac tccggagcag
     2221 cgagctgttt acgaaattgt cctcgaagcc aatcgcaccg cgattgccaa ggtcagaccc
     2281 ggcaatcact ggaatgagcc ccatctcgct gccgtgcacg tcattacaca gggactggtg
     2341 aggctgggcc tgttgaaagg tcgcgtcgcg aagctcgagc gcgatggcgc gtaccggcgc
     2401 ttcttcatgc accggacggg ccactggctg ggaatggacg tgcacgacgt cggcgactac
     2461 aagatcggcg atgagtggcg cgtgtttgag ccaggaatgg tgctgaccgt ggaacccggc
     2521 atctacatcc ccgcgagcac acgcggggtt ccaagacggc tgcgcaacgt tggggtgcgg
     2581 atcgaggacg acgtggtggt cacgcgtacg ggggccgaag tgctgacggc ccgagcgccc
     2641 aaggacgcgg atgccatcga atcgctcatg tcttcaggct gatgcgatga gcgccgcggg
     2701 cgtaaccgca agccggaacg aagctcaccc gcctactcta gaggttgacg tcgcgatcat
     2761 cggaggcggg atggtggggg caagccttgc gcttgcgctc gaaggctccg ctctcaagac
     2821 actgctcgtg gagagtgttg ctcccggctc gaaggcgcaa ccgagcttcg atgagcgcac
     2881 gact
//
LOCUS       DASRHN010000256         4881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2065635, whole genome shotgun sequence.
ACCESSION   DASRHN010000256 DASRHN010000000
VERSION     DASRHN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065635"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFB37_10145"
     CDS             <1..656
                     /locus_tag="VFB37_10145"
                     /inference="COORDINATES: protein motif:HMM:NF016088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HZO22851.1"
                     /translation="IPQYFTLLLNLNFVPYWPVLAFTIVYHAELVAAVFGGKYIAQSW
                     LLPLSVLFMMISAIATPVTLVAQYEERAGLILLSKITALYNIAALLVLVPVWGLYGAM
                     LARGSAVALKNGFIWWFVRRRAVWVNFVPFMLTGVLLWGSVTLVCYAMKTYIEVPRIV
                     HLVLGAVVCALAVPIYTRTRAICRSDREILASLFRGREARPLEWIGLLRPVGNGGTA"
     gene            653..1924
                     /locus_tag="VFB37_10150"
     CDS             653..1924
                     /locus_tag="VFB37_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO22852.1"
                     /translation="MSGSWEGGSGPAHSRPFRLLMLASDTYPPQRVDVSILFGEELAA
                     RGHRIDLILQSEAACERGYIAPWGGGQVWVAPADRGDSLLRRLRKHLSSIRHDAKLFG
                     LMRSGRYDAVEVKDKFISALFAAVAARLYHKRFIYWLSYPFPEFYLMRAKESSARYPL
                     LYRIRGEAFRFLLYRIVLPAADHVFVQSEKMRKDVAAQGIPLSKLTAVPMGIRTEGLS
                     ASPEPTDRSVISPNEPSVLYLGSLARERHIEFLLRTFARVLEEMPQAKLYLVGSAEDP
                     REQQYLVDEAKRLGVLQAVVFSGQLPQREAFRFVREADVCVSPLYPTPIYEVATPTKL
                     VEYMALGKAVVANTHPDQRMLIEESGCGYCVPYEEQAFAQAVLKLLRAPELAREMGER
                     GRRYALEKRGYPAIADLVEREMQRIVAGVHP"
     gene            1921..3045
                     /locus_tag="VFB37_10155"
     CDS             1921..3045
                     /locus_tag="VFB37_10155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO22853.1"
                     /translation="MSATASGRKRLCIVTPYQSDQPGGAEYQIECLLQTLIAQQRYEI
                     YYLAAVVSGTEAHADYRVVRIGRSPRMPRFGYVTHTRALYRTLAELAPDVIYQRVGCG
                     YTGIAAHFAQRHGTKLVWHVSSDTDVTPEKSLDGRNPIRRFLEKRCVEYGIRRASCIV
                     TQTQHQARLLEMNYRRKPDAIIPNFHPEPSEPLDKSGPLTVAWVSSLKPFKQPEAFLE
                     LADSLRDRADVRFVMIGPWQGEASWVESVRPRIESTPNLEYLGARTQEEVNALLARAH
                     VFVNTSLYEGFPNTFIQAWMREVVVCSLHVDPDGLLERAGLGVFCHDSRAQLNESVRR
                     LLTDSAQRTALAQRGRAYAHSMHSLANAQSLVELLEAKCA"
     gene            3042..4328
                     /locus_tag="VFB37_10160"
     CDS             3042..4328
                     /locus_tag="VFB37_10160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO22854.1"
                     /translation="MSTLVSINNYYYERGGSEAVFFGHNRMLEAQGWNVVPFSMKHPR
                     NIPSPWSDYFVDELEMSGQYSMAQKLVRLPKVIYSLEARTKLSRLLDRVRPDVAHGHN
                     IYHHISPSILGLLKSRGIPTLLTLHDLKIACPAYSMMAPDGICERCKGGRVYNVVAHR
                     CIGNSAAMSFIVMLEAVVHRLIGSYRDCVSRFIVPSRFYIDKFSEWGLPRSQFRYVPN
                     FVHAPGFQPNPTPGKAFIYFGRLTPQKGVATLIRAVAEARTPLVVAGTGPDMEAMQAL
                     TASLGADVSFLGHLSGSMLHDTIRAARAVVLPSEWYENAPMSLLEGYALGKPVIGARI
                     GGIPELVREGETGVCFESGNVASLVAALRDMASRPDDELTQMGQSGRRWVEQDFTLDR
                     YRQRILAAYRELGIRIDSIPSTDTLRLESFASGEGE"
     gene            4339..>4881
                     /locus_tag="VFB37_10165"
     CDS             4339..>4881
                     /locus_tag="VFB37_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO22855.1"
                     /translation="MVLGVRSLSGVQGGVETHAEQLYPRLAKLGCDVEVLVRTPFTPR
                     KHKWLGDVRLRRIWSPRTPGFEALVHSILGVLYAGVSRPDVLHIHAVGPSIVAPIARL
                     MGLRVVVTHHGPDYDRDKWGWFARWVLRSGESLGMVYANARIAISKVIVDAVRSRYGR
                     DSDLIPNGAVSVEMPRETNEI"
BASE COUNT          777 a         1581 c         1609 g          914 t
ORIGIN      
        1 gcatcccgca gtacttcacg ctgctgctga atttgaactt cgtgccctac tggccggtgc
       61 tcgccttcac gatcgtctac cacgcggagc tcgtggccgc ggtcttcgga ggtaagtaca
      121 tcgcccaatc ctggctgctg cccctgtcgg tgctgttcat gatgatcagc gcgatcgcca
      181 cacctgtgac gctcgtggcg cagtacgagg agcgcgccgg gctgatcctg ctgagcaaga
      241 tcacggcgct ctacaacatc gcggcgctgc tggtactcgt gcctgtctgg ggcctctatg
      301 gtgcgatgct cgcgcgcggc tcggcggtgg cgctgaagaa tggcttcatc tggtggttcg
      361 tgcgccggcg ggcggtctgg gtcaacttcg ttccattcat gctgaccggc gtgctgctct
      421 ggggcagtgt gacgctcgtc tgctacgcca tgaaaaccta tatcgaggtg ccgcgaatcg
      481 tgcaccttgt gctgggggcg gtcgtgtgtg cgctcgccgt gccgatctac acgcgcacgc
      541 gggccatctg ccgctcggac cgcgagatcc tcgcctcgct gttccgcggc cgcgaggcgc
      601 ggccgctgga atggatcggg ctgcttcgcc ctgttggcaa cggcgggacc gcatgagcgg
      661 gtcgtgggag ggaggatcgg gcccggccca ctcacggccg ttccggctgc tgatgctggc
      721 ctccgacacc tatcctccgc agcgcgtcga tgtgtcgatt ctgttcggcg aggagctcgc
      781 cgcccgcgga caccggatcg atctcatcct gcagagcgag gccgcctgcg agcgcggcta
      841 catcgcgccc tgggggggcg gacaggtgtg ggtggccccg gccgatcggg gcgattcgct
      901 gctgcgtcgg ttgcgcaagc acctctcgag catccgtcac gatgccaagc ttttcggctt
      961 gatgcgcagc ggccgctacg atgcggtcga agtgaaggac aagttcatct cggctttgtt
     1021 cgcagccgtg gccgcgcgcc tctaccacaa gcgcttcatt tactggctct cctatccctt
     1081 tccggagttc tacctgatgc gcgcgaagga aagctcggcg cgctatccgt tgctgtatcg
     1141 cattcgcggt gaagccttcc gcttcctgct gtatcgcatc gtgctgcccg cggcggatca
     1201 tgttttcgtg cagagcgaga agatgcgcaa ggacgtggcc gcgcaaggca ttccgctctc
     1261 gaagctgacc gctgtgccga tgggcatccg caccgagggc ctgagtgcct cgcccgagcc
     1321 caccgaccgc agcgtgatct caccgaacga gcccagtgtg ctctacctcg ggtcgctggc
     1381 gcgcgagcgg catatcgagt ttctgctgcg caccttcgcg cgggtgctcg aagagatgcc
     1441 gcaggccaag ctctatttgg tcgggagcgc cgaggatccg agagagcagc agtacctggt
     1501 cgatgaggcg aagcgcctcg gcgtactgca agcagtggtg ttctccggtc agctgccaca
     1561 gcgcgaggct ttccgcttcg tgcgcgaagc cgacgtttgt gtctcaccgc tgtaccccac
     1621 gccgatctac gaggttgcga ctccgacgaa gctggtggag tacatggcgc tcggcaaggc
     1681 ggtggtggcc aacacccacc cggatcagcg catgctgatc gaggaaagcg gttgcggcta
     1741 ttgcgtcccc tacgaggagc aggccttcgc ccaggccgtt ctcaagctgt tgcgtgcccc
     1801 ggagctcgcg cgcgagatgg gtgaacgcgg ccggcgctat gcgctggaga agcgcggcta
     1861 cccggccatc gccgatctcg tcgagcgtga gatgcagcgc atcgtcgcgg gagtgcaccc
     1921 gtgagcgcca cagcgagcgg ccgcaagcgg ctgtgcatcg tgactcccta ccagtcagat
     1981 cagcccggcg gtgccgagta ccagatcgag tgcctgctgc agaccctcat cgcgcagcag
     2041 cgctatgaga tctattacct ggccgcggtc gtctcgggca cggaagcgca cgcggactat
     2101 cgggtcgtgc gcatcgggcg ctcgcctcgg atgccgcgct tcggctacgt gacgcacaca
     2161 cgagcgctct accgcacgct ggctgagctt gcgccggatg tcatttacca gcgggtcggt
     2221 tgcggctata ccgggattgc ggcgcatttc gcgcagcgtc acgggacgaa gctcgtctgg
     2281 cacgtctcct cggacaccga cgtgacgccg gagaaatccc tcgacgggcg caatcccatc
     2341 cggcgcttcc tcgagaagcg ctgcgtggaa tacggcatcc gccgggcgag ctgcatcgtc
     2401 acccagactc agcaccaggc ccggctgctg gagatgaact atcggcgcaa gcccgatgcc
     2461 atcatcccga acttccatcc cgagccgagc gagccgctgg acaagtccgg cccgctcacc
     2521 gtggcctggg tctccagtct caagcccttc aagcagcccg aggctttcct cgagctcgcc
     2581 gactcgctgc gcgaccgcgc cgatgtccgc ttcgtcatga tcggcccctg gcagggcgag
     2641 gcgagctggg tcgaatccgt gcgcccgcgg atcgagtcca cgcccaacct cgaatatctc
     2701 ggggcgcgca cgcaggagga ggtcaatgcg ctgctcgcgc gggcccacgt gttcgtgaac
     2761 accagtctct acgaaggctt ccccaacacc ttcatccagg cttggatgcg cgaggtcgtc
     2821 gtgtgcagcc tgcatgtgga ccccgacggc ctgctcgagc gcgcaggcct tggggtcttc
     2881 tgtcatgact cgcgagcgca gctcaacgag tcggtccggc gcctgctcac cgattcggcc
     2941 cagcgcaccg cgctcgctca acgcggccgg gcgtatgccc attccatgca ttccctggca
     3001 aacgcgcagt cgctcgtgga gctgctggag gcgaagtgcg catgagcacc ctcgtctcca
     3061 tcaacaatta ctactacgag cgcggcggct ccgaggccgt cttcttcgga cacaaccgca
     3121 tgctcgaggc gcagggctgg aacgtggtgc ccttctccat gaagcacccg cgcaatattc
     3181 cctccccctg gtcggattat ttcgtggatg agctggagat gagcggccag tactcgatgg
     3241 ctcagaagct ggtgcgactg ccgaaggtga tctactcact cgaggcgcgc acgaagctca
     3301 gccggctcct cgatcgcgtg cggcccgatg tcgcgcacgg ccacaacatt taccaccaca
     3361 tctcgccatc cattctcggg ctgctcaaga gccggggcat tccgacgctc ctcaccctgc
     3421 atgacctcaa gatcgcctgc ccggcctaca gcatgatggc cccggatggg atctgcgagc
     3481 gctgcaaggg aggccgggtg tacaacgtgg tggcgcaccg ctgcatcggc aattctgcgg
     3541 cgatgagctt catcgtgatg ctggaagcgg tggtgcaccg gctgatcggc tcgtaccgcg
     3601 actgcgtgag ccggttcatc gttcccagcc gattttacat agacaagttc agcgagtggg
     3661 gtctgccgcg ctcgcagttc cgctacgtgc cgaacttcgt gcatgccccg ggctttcagc
     3721 cgaacccgac gccaggcaag gcgttcatct actttgggcg gctcaccccc cagaagggcg
     3781 ttgcgacgct gatccgcgcc gtcgcagagg cgcgcacgcc gctggtggtg gcaggtaccg
     3841 gcccggatat ggaggctatg caggccctta cagccagcct gggcgcggat gtcagctttc
     3901 tcgggcatct cagcggcagc atgctgcacg acaccattcg ggccgcccgg gcggtcgtgc
     3961 tgccgtcgga gtggtacgag aacgccccca tgagcctcct ggagggctat gcgcttggaa
     4021 agcccgtcat tggcgctcgt atcgggggca tcccggagct cgttcgcgaa ggcgagacgg
     4081 gcgtatgctt cgaaagcggc aatgtggcct ccctggtggc ggccctgcgg gacatggcgt
     4141 cccggccgga cgacgagctc acgcagatgg gccagtccgg acgacgctgg gtggaacagg
     4201 actttacact cgacaggtac cggcagcgca tcctggcggc gtaccgggaa ctgggtatcc
     4261 ggatagacag tattcccagc acggacacat tgcggttaga gtcgttcgcg agcggggaag
     4321 gggaatagct tgagggtcat ggtactgggc gtccggagcc tctccggcgt gcaaggcgga
     4381 gtggagactc acgcggagca gctttatccg cgtctggcga agctcggctg cgacgtcgag
     4441 gtcctcgtgc gcacgccgtt cacgccccgc aagcacaagt ggctcggcga tgtgcgtttg
     4501 cggcgcatct ggtcgccgcg cacgccgggg ttcgaggcgc tcgtgcactc gattctgggc
     4561 gtgttgtatg ccggcgtgtc acggccggat gtgttgcaca ttcacgccgt gggaccctcg
     4621 atcgtcgcgc cgatcgcgcg cctgatggga ctgcgcgtcg tggtcacgca ccatggcccg
     4681 gattacgatc gggacaagtg gggttggttt gcgcgctggg tgctgcgtag cggcgagagc
     4741 ctcggcatgg tctacgccaa cgcacggatc gcgatttcca aagtgatcgt cgatgcggtg
     4801 cgcagccgct atgggcgcga ttccgatctg attcccaatg gggcggtctc ggtcgagatg
     4861 ccgcgcgaga ccaacgagat c
//
LOCUS       DASRHN010000257        13000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_250177, whole genome shotgun sequence.
ACCESSION   DASRHN010000257 DASRHN010000000
VERSION     DASRHN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13000
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250177"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(403..918)
                     /locus_tag="VFB37_10170"
     CDS             complement(403..918)
                     /locus_tag="VFB37_10170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO22856.1"
                     /translation="MTVSVRDARPSESDRLWIGGIYREYLDDLSVQNTGLFPTLGEIG
                     HREPDQIARWFGDRTAVPLVILKSDERVGFALVARGASAPARTPIEYRMAEFFIVRAS
                     RRLGIGRTAVQLILNRFAGTWEITEYLRNPAAVSFWRRVVAGYTLGRYQERVINGEVR
                     QVFDSRGERPR"
     gene            1619..2980
                     /gene="pepQ"
                     /locus_tag="VFB37_10175"
     CDS             1619..2980
                     /gene="pepQ"
                     /locus_tag="VFB37_10175"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HZO22857.1"
                     /translation="MSDCNLPSTRADLDASFGQHLRRVGERTARALEASGCDSLLVHS
                     GSPLTVFEDDRTYPFEAHAPFKVWVPLSDAPDCLLFFAPGKQPRLLFHSPPDYWYKPA
                     SLPQAYWTRHFEIVPVVDRAAARQALPRDLSRTAYIGDALTELQNWGVKAVNPPELMH
                     RLDFSRALKTPYELECLREANRLGALGHLAAAEAFRSGASEFEIELAFLRACGLREQE
                     LPYNPIIALNAGGSVLHYQVLEKQPPEQRHSLLIDAGAEFGGYASDITRTHALDAGDF
                     RSLIARMDEMQQTLCASVRSGVDWREVHLRAHELIATLLRDSDITTCTSEEAVATGVS
                     KVFFPHGLGHLLGLEVHDVGGFMRSPEGGEIPRPDGHPYLRLTRVLEPGFVVTMEPGI
                     YFIEQLLSEARADARASRINWSRVADLSRFGGIRIEDDLAVTAAGCENLTRDAFSAIE
                     ARS"
     gene            complement(3013..3414)
                     /gene="mscL"
                     /locus_tag="VFB37_10180"
     CDS             complement(3013..3414)
                     /gene="mscL"
                     /locus_tag="VFB37_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015379263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZO22858.1"
                     /translation="MSLGSEFKEFAMKGSVIDLAVGVVIGAAFGKIVASLVGDVIMPP
                     LGLVIGSVNFSDLAAHIGTDPTGKAVLVKYGAFVQTVFEFLLIALVLFIVIKGINRLK
                     RPPPAVAAPAPPAPSRSEVLLQEIRDLLAKR"
     gene            complement(3750..4106)
                     /gene="rplQ"
                     /locus_tag="VFB37_10185"
                     /pseudo
     CDS             complement(3750..4106)
                     /gene="rplQ"
                     /locus_tag="VFB37_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(4187..5191)
                     /gene="rpoA"
                     /locus_tag="VFB37_10190"
     CDS             complement(4187..5191)
                     /gene="rpoA"
                     /locus_tag="VFB37_10190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZO22859.1"
                     /translation="MQGSVTEFLKPRVVKVQPMAPRQARVVIEPFERGFGHTLGNALR
                     RVLLSSMPGSAITEVEIDGVLHEYTSIEGVQEDVVDILLNLKQVAIRMHARDSAELRL
                     HKKGPGPVTAADIQTDHDVEVVNPDLVLANLTKAGELSMNLRIERGRGYRPAAARAAF
                     EEQTRPIGRLQLDASFSPVRRVTYSVDAARVEQRTDLDKLVIDIETNGTIDAEEAIRR
                     AGSILKDQLSVFVDLQGEEEAATKVSEMQFDPILLRPVDELELTVRSANCLKAENIHY
                     IGDLVQRTEVELLRTPNLGKKSLTEIKEVLQSHGLMLGMRLDGWPPAGLKHDEERDVI
                     "
     gene            complement(5292..5915)
                     /gene="rpsD"
                     /locus_tag="VFB37_10195"
     CDS             complement(5292..5915)
                     /gene="rpsD"
                     /locus_tag="VFB37_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZO22860.1"
                     /translation="MARYVGPKCKLARREGTDLFLKSGVKPLESKCKFTVPPGGIKGE
                     RRQRLSDYGLQLREKQKLRRMYGVLERQFANYYEEAARRIGATGENLLRLLECRLDNV
                     VYRMGFAATRAEARQLVSHKAITVNERPVTIASYQCKAGDVVQVREKSRKQLRIQSAL
                     NVAQQVGLPDWLEVNDKEFRGVFKSAPAREDVLPDINENLVVELYSK"
     gene            complement(6055..6477)
                     /gene="rpsK"
                     /locus_tag="VFB37_10200"
     CDS             complement(6055..6477)
                     /gene="rpsK"
                     /locus_tag="VFB37_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZO22861.1"
                     /translation="MADQKSGGAAAGAASAAKKPKKARKNVLDAIAHVHASFNNTIIT
                     ITDRQGNTLSWATSGGCGFRGSRKSTPFAAQVAAEKAGVAAQEHGVKNVEVRVGGPGP
                     GRESAVRALNNCGLKITSIADVTPIPHNGCRPPKKRRV"
     gene            complement(6494..6874)
                     /gene="rpsM"
                     /locus_tag="VFB37_10205"
     CDS             complement(6494..6874)
                     /gene="rpsM"
                     /locus_tag="VFB37_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZO22862.1"
                     /translation="MARIAGINIPMNKHVWIGLTHIFGVGRARARMACEAAGVDPTTK
                     VKDLTEPEVNAIRSQIAKFAVEGDLRREVSMNIKRLMDLGSYRGIRHRRGLPVRGQRT
                     RTNARTRKGPRKQAVKLNAPPGKA"
     gene            complement(6965..7078)
                     /gene="rpmJ"
                     /locus_tag="VFB37_10210"
     CDS             complement(6965..7078)
                     /gene="rpmJ"
                     /locus_tag="VFB37_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZO22863.1"
                     /translation="MKVRPSVKKICKNCKIVRRRRVVYVICSDPRHKQRQG"
     gene            complement(7095..8447)
                     /gene="secY"
                     /locus_tag="VFB37_10215"
     CDS             complement(7095..8447)
                     /gene="secY"
                     /locus_tag="VFB37_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZO22864.1"
                     /translation="MANSTFSNPAAALGEAARFGEVRGRLFFLIAALVVYRIGTFIPV
                     PGINPSEVARFFSDQSNTILGIVNMFSGGALSRLSIFAMGVMPYISASIIIQMMSMVI
                     PQLQELRKEGESGRRKITEYTRYGTVVLAAFQSFAAAGALVKGGLTLVGGWQFLFTAT
                     VTMTTGTMFLMWLGEQITERGIGNGISMIILAGIVAGLPGAIGNTAEAVNTGEMSGPF
                     ALLLIILVLGTTAFVVFVERAQRRIAVNYAKRQVGRRMYAGQSSHLPFKLNMSGVIPP
                     IFASSLLLFPATLASFAGSGSQGPFAIFMQDVSQALGYGQPLHLVIYALLIVFFCFFY
                     TALVFNARDTADNLKKSGAFIPGIRPGQQTAEYIDKVLTRLTLWGALYVAAVCLLPEI
                     LVSSQGVPFHFGGTSLLIVVVVVMDFMAQLQAHLMSHHYPGLLKKANLLGYGRTGPGG
                     "
     gene            complement(8705..9133)
                     /gene="rplO"
                     /locus_tag="VFB37_10220"
                     /pseudo
     CDS             complement(8705..9133)
                     /gene="rplO"
                     /locus_tag="VFB37_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(9162..9365)
                     /gene="rpmD"
                     /locus_tag="VFB37_10225"
     CDS             complement(9162..9365)
                     /gene="rpmD"
                     /locus_tag="VFB37_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005307985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZO22865.1"
                     /translation="MADKAQVKVTLIKSLHGQLASIAACARGLGLRRIGQTVTVADTP
                     ENRGMIHGAMHLLKVDRSGGRAG"
     gene            complement(9371..9877)
                     /gene="rpsE"
                     /locus_tag="VFB37_10230"
     CDS             complement(9371..9877)
                     /gene="rpsE"
                     /locus_tag="VFB37_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZO22866.1"
                     /translation="MARPEKAQSGDEFVEKLVAVNRTAKVVKGGRQFGFTALTVVGDG
                     SGRVGYGFGKAREVPVAISKAMAQARKNLINVALRNDSLHYAVKGVHGATRVYMQPAS
                     DGTGVIAGGGMRAVLECAGVRNVLAKSYGSRNPINVVRATIKALGSVRSPDDIAAKRN
                     KTLEEIIG"
     gene            complement(9887..10240)
                     /gene="rplR"
                     /locus_tag="VFB37_10235"
     CDS             complement(9887..10240)
                     /gene="rplR"
                     /locus_tag="VFB37_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZO22867.1"
                     /translation="MTKKQRRLRRAVKTREHIRQLDVARLTVHRTPRHIYAQVTAADG
                     ARVIATASTLQEGVRQGLKGTGNVEAAKAVGRVIAERTKAAGVGKVAFDRAGFQYHGR
                     IKALAEAAREAGLEF"
     gene            complement(10243..10773)
                     /gene="rplF"
                     /locus_tag="VFB37_10240"
     CDS             complement(10243..10773)
                     /gene="rplF"
                     /locus_tag="VFB37_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZO22868.1"
                     /translation="MSRIAKAPVDLPQGVTATVSADSVTIKGSKGSLSVPLGRGVSVV
                     QTDKKLQIKYDDPGLARTQAGATRAHLANMVRGVTKGYERKLELVGVGFRAQVQGKSL
                     NLTLGFSHPVNFAIPEGITIEAPSQTEIIVRGIDRQKVGQVAAEIRDLRPPEPYKGKG
                     VRYTGEQIILKEGKKK"
     gene            complement(10799..11194)
                     /gene="rpsH"
                     /locus_tag="VFB37_10245"
     CDS             complement(10799..11194)
                     /gene="rpsH"
                     /locus_tag="VFB37_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZO22869.1"
                     /translation="MSMTDPIADLLTRIRNGQTAGKSEVQLAASRLKTAIVKVLKEEG
                     YIADFRTESDGGKPTLTIGLKYYEGRPVIDRIERVSRPGLRIYRGKDQLPKILGGMGT
                     VIVSTPKGVMTDRQARSIGQGGEVLCIVA"
     gene            complement(11205..11510)
                     /gene="rpsN"
                     /locus_tag="VFB37_10250"
     CDS             complement(11205..11510)
                     /gene="rpsN"
                     /locus_tag="VFB37_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HZO22870.1"
                     /translation="MAKTSMVQRERRRAKIVKQYAAQRAQLKELIRSPRTSPEERMAA
                     QSKLQSLPRDASPSRQRKRCAITGRSRGVYRKFGLSRVKIREVASRGEIPGLRKASW"
     gene            complement(11644..12183)
                     /gene="rplE"
                     /locus_tag="VFB37_10255"
     CDS             complement(11644..12183)
                     /gene="rplE"
                     /locus_tag="VFB37_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZO22871.1"
                     /translation="MARLQQYYRETVVPRLRSDLKIGNPMQVPRISKITVNMGVGEAV
                     ADRKVMDAAITDLTKITGQKPLITKSRKAIASFKLRAGLAVGAKVTLRGVRMYEFLDR
                     LINIAMPRIRDFRGVSPRAFDGQGNYSLGVKEQIIFPEIQYDQVDQIRGMDITITTTA
                     TDDRHGRALLEAFNFPFRK"
     gene            complement(12206..12523)
                     /gene="rplX"
                     /locus_tag="VFB37_10260"
     CDS             complement(12206..12523)
                     /gene="rplX"
                     /locus_tag="VFB37_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZO22872.1"
                     /translation="MKKIRKGDQVVVLAGRDKGRRGAVVQVLGDGRVLVESVNRVKRH
                     QKPNPQANRQGGIIEKEMPLPVSKVAIWNPGAKKADRIGFKTLADGKKVRFFKSNGEM
                     LDA"
     gene            complement(12544..12912)
                     /gene="rplN"
                     /locus_tag="VFB37_10265"
     CDS             complement(12544..12912)
                     /gene="rplN"
                     /locus_tag="VFB37_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZO22873.1"
                     /translation="MIQLRTMLNAADNSGARTLMCIKVLGGTRRRYATVGDVIKVSVK
                     DAIPRGKVKKGEVYDAVVVRTTHGVRRPDGSLIRFDGNAAVLLTNKLEPIGTRIFGPV
                     TRELRNVQFMKIISLAPEVL"
BASE COUNT         2097 a         4354 c         4033 g         2516 t
ORIGIN      
        1 gctcgcctgg tacgtgttcg caccacagtg tctgtccacg ctcgcagtcg tgcgtcgcga
       61 gacgaactcg tggcgctacc cgctgctgat ggccggctat cttttcgccc tggcctatgt
      121 gggggcgttc gtgacgtatc aggtcgcagt cgccgccgga gccggatgag atggcctctc
      181 tcgagaccgt cctcgccgtt ctcatcgtga tcgcttgtgc ggccttcagc ctctggcgac
      241 tgatgtcggc gcggctgcgc ctgcggctat tggacctgag ggtgctcgat agtttgaggg
      301 gaggtcgcgc gcctcaggcc ggctggcgag cccggctccg tgagaaggcg ttggccgagc
      361 tcagcggcgg gtgcggagcc tgctcgcgcg cgccgcgctc ccttaccgcg gtctctcccc
      421 ccgcgagtcg aaaacctgcc gcacctcccc gttgatgact ctttcctgat atcgcccaag
      481 ggtgtaaccc gccacgaccc gtcgccagaa gctgacggca gccgggttgc gcagatattc
      541 ggtgatttcc caggtccccg cgaaacgatt gaggatgagc tgaacggccg tgcgtccgat
      601 gcccaggcgc cggctggccc gcacgatgaa aaactccgcc atgcggtatt cgatcggcgt
      661 gcgcgccggg gctgacgcac cgcgcgccac cagtgcgaag ccgacccgct cgtcggattt
      721 caggatgacg agcggaactg cggttcgatc accaaaccag cgggcgatct gatcgggctc
      781 gcggtgaccg atctcgccga gcgtggggaa cagacccgtg ttctgcacgg agaggtcatc
      841 gagatattcc cggtagatgc cgccaatcca caggcggtcg ctctcggaag gtcgggcatc
      901 gcgcacgctg acggtcacgg ccgcatttcc gctcaggaaa gaggcctacg gctggccgaa
      961 aagacacggc ccggacagct cgcggatgcg atctgtccag gccgtcttga gccctcggct
     1021 cgggtaagta ggtctttcga gctacttact gcttcggcgg cgcggcaggc gcattcgaat
     1081 ccgccggagg cggcgtcgca gcgttgtcgg ccggcggcgg agcagcagcg ctgctgtcag
     1141 aagctggcgc ggcaggcgca ttgtcggccg gagcggcggg agccggagcg ctggcggccg
     1201 gagcggctgc ggaatcagtg ctggcggctt cctgcttcga gcaggccgtc agcatcagcg
     1261 ccgcaaccaa tgcgctaaga acgagtttga tattcatcga tcgttacccc gaagagcagc
     1321 aagagtggtg tgaaaacact atgaacgatt cttttcgttc cgcggatcct gcaccggatc
     1381 gcgtcgataa attctagcga ttccccggtc gatttgaaac agccgcgccg ggattgtctg
     1441 cctattacga caggtgaggt cctgagtttg tgtcccttgg gcaacgcacc cctcgcaccc
     1501 cggctgccca tgcaggaaac gcagcctcca gaagcactcg ccgatgccgg ggggccgcag
     1561 cagcggccga gggggtaacc taccgcatca gatcaggcct ttctgatggg atctcaccat
     1621 gtccgattgt aatctgcctt ccacccgagc cgatctcgat gcctcctttg ggcagcattt
     1681 gcgccgcgtc ggtgagagaa ctgcgcgggc gctggaggca agcggctgcg acagtctgct
     1741 ggtgcactca ggatcgccgc tgaccgtatt cgaggacgat cggacctacc cgttcgaggc
     1801 gcatgcgcct ttcaaggtgt gggtgccgct ctcggacgct ccggattgcc tgctgttctt
     1861 cgcgccaggc aagcagcccc ggctgctgtt ccatagtcca cccgactact ggtacaagcc
     1921 cgcctcgctg ccgcaggcat actggacccg gcacttcgag atcgtgcccg tcgtcgatcg
     1981 cgccgccgct cgccaggcgc tcccgcgaga cctctcccgc acggcctata tcggtgatgc
     2041 cctcacggag ctgcagaact ggggcgtcaa ggcggtcaac ccgcccgagc tgatgcaccg
     2101 cctggatttc tcacgcgcgc tcaagacacc ctacgagctc gaatgcctgc gtgaggccaa
     2161 tcgcctgggc gctttgggac acctcgctgc cgccgaggcc ttccggtccg gcgcctcgga
     2221 gttcgagatc gagctggcat tcctgcgtgc gtgcggtctt cgcgagcagg agcttcccta
     2281 taatcccatc atcgcgctga atgctggcgg ctctgtgttg cactaccagg tgctggaaaa
     2341 gcagccgccc gagcagcggc attcgttgct catcgatgcc ggcgccgaat tcggcgggta
     2401 tgcgtcggat atcacgcgta cccacgcact cgacgcgggg gatttcagat ccctcatcgc
     2461 gcgcatggac gagatgcaac agaccctgtg tgcttcggtg cgttccggtg tggactggcg
     2521 tgaggtgcac ctgcgcgcac acgagctgat agccaccctg ctgcgcgact ccgacatcac
     2581 gacctgcaca agcgaggaag ccgttgccac cggcgtgtcg aaggtgtttt tcccgcacgg
     2641 cctcggtcac ctgctcggcc tggaagttca cgatgtaggt gggttcatgc gctcaccgga
     2701 agggggcgag atcccgcggc ccgacggaca tccctatctg cgcctgacgc gcgtcctcga
     2761 gcccggcttc gtggtcacga tggagccggg catctacttc atcgaacagc tgctgagcga
     2821 ggcgcgagcc gatgcacgcg cgagccggat caactggtcg cgtgtggcgg atctgagccg
     2881 ctttggtggc atccgcatcg aagatgacct ggcggtcacc gcagccggct gcgagaacct
     2941 gacccgggat gcattcagcg cgattgaggc ccgctcctag gccatcgtca cctcaaccgg
     3001 gcaccgccaa cctcagcgtt tcgcgagcag gtcccgtatt tcctgcagca gcacttccga
     3061 gcgcgatggc gccggcggcg caggcgcggc gacggcaggc ggaggccgct tgagccggtt
     3121 gatgcccttg atcacgatga acagcacgag cgcgatgagc agaaactcga acaccgtctg
     3181 aacgaaggcg ccgtacttca ccagcactgc tttgccggtc ggatcggtcc cgatgtgcgc
     3241 cgcaagatcg ctgaagttga cactcccgat gaccagcccc agcggcggca tgatcacgtc
     3301 cccgaccagg gaggcgacaa tcttgccgaa cgcggcgcca atcacgacac cgaccgccag
     3361 gtcgatgacg cttcccttca tggcgaattc tttgaattcg gatccaaggc tcatgattct
     3421 tcccctgttg gcgattgtta ttgggcagcg cgtcgggagc ctacgtaaac aggtatacgt
     3481 acgtcaattc gagagccgac atcccgagcg cgtgccgggc gtcacgagcc cgagacaggc
     3541 cccattgccg ctgggccctt gtctggaggc tgtccggccg cctcgtcggc ggccagtctc
     3601 ggcaatcagg ccgctgcggc cttcttcttg cgcgttcgct tgggcttggc ctccggctcg
     3661 ggagccgcgg cctcggctga tgccttcttc ttcacacgcc gggcggtctt tttcttcttc
     3721 cctgccggct cctcggcagc caccggcaca ggcccgtcca ccagctgcat gagcgccatc
     3781 ggcgccgagt cgcccgcccg cggcagcatg cgcagaatgc gggtatagcc gccgggacgc
     3841 gccttgaacc gtggtcccag atcggcgaac aacttctcca cgatctgtgc atcccgcagc
     3901 cgcgagaaag ccagacggcg gttggcatct ccgtcggtct tggccagcgt gatgagcggc
     3961 tcgactaccc ggcgcagctc cttcgccttg ggcagagttg tgcgaatcgt ctcgtgccgc
     4021 agcagcgaaa cgctcatgtt gcgcagcatg gcgtgccgat gcgagctgtt gcgcgacagt
     4081 tgccggccag atagttggtg acgcatattt cagccttgtc gtggcctgcg gccatactca
     4141 ccggagatca gcccccggcg aaattcaacg gtgcgacgcg ccgtgatcag atcacgtcgc
     4201 gctcttcatc gtgcttcagc ccggcgggcg gccagccgtc cagcctcatg cccagcatga
     4261 ggccgtggct ctgcagcact tccttgattt cggtcagcga cttcttgccc aggttgggcg
     4321 tccgcagcag ctcgacctcg gtgcgctgca cgagatcacc gatgtagtga atgttctcgg
     4381 ccttgaggca gttcgcgctg cgcacggtga gctccagctc gtccaccggc cgcagcagga
     4441 tcggatcgaa ctgcatctcg gacaccttgg tcgcggcctc ttcctcacct tgaaggtcca
     4501 cgaacacgga gagctggtcc ttgagaatgc tgccggcgcg gcggatggct tcttccgcgt
     4561 cgatcgtgcc gttggtctcg atgtcaatga cgagcttgtc gaggtcggtc cgctgctcga
     4621 cacgcgccgc atccaccgag taggtaaccc ggcgcaccgg ggagaacgag gcatcgagct
     4681 gcagccgtcc gatcggtcga gtctgctcct cgaaagcggc acgggctgcg gcgggccgat
     4741 agccgcgtcc gcgctcgatc cgcaggttca tgctcagctc gcccgccttg gtcaggttgg
     4801 ccagcacgag gtccggattg acgacttcga cgtcgtgatc cgtctgaata tccgcggcgg
     4861 tgaccgggcc ggggcctttc ttgtgcaggc gcagctcggc gctgtcgcgc gcgtgcatgc
     4921 ggatcgcgac ctgcttcaga ttgagcagaa tgtcgaccac atcctcctgg acaccttcga
     4981 tgctggtgta ctcgtgcagg acgccatcaa tctctacctc ggtgattgca ctaccgggca
     5041 tggacgacag caggacgcgt cgcagtgcat tacccagggt atgcccgaag ccgcgctcga
     5101 atggctcgat gaccacgcgt gcctggcgcg gagccatcgg ctgcaccttg acgacgcgag
     5161 gcttcaagaa ctcggtgact gaaccctgca tagcatctca ctttgccggc tgatctggcc
     5221 ggcgccaaaa taacactaca atctgttgga tccgcgctgg gaccgcgctg gcggcccgcg
     5281 cagcgcgcaa atcacttcga atagagctcg acgacgaggt tctcgttgat gtcgggcagc
     5341 acgtcctcgc gcgccggcgc cgacttgaaa acaccgcgga actccttgtc gttcacctcc
     5401 agccagtcgg gcagcccgac ctgctgagcg acgttcaacg cgctctggat gcgaagctgc
     5461 ttgcgcgact tctcgcgcac ctgcaccaca tcgccggcct tgcactggta ggaggcgatg
     5521 gtgacggggc gctcgttgac cgtgatcgcc ttgtggctca cgagctggcg cgcctcggcc
     5581 cgcgttgccg cgaaacccat gcgatacacg acattgtcga gccggcactc gagcagccgc
     5641 agcaggttct cgccggtcgc accgatgcgg cgtgcggcct cctcgtaata gttcgcgaac
     5701 tggcgctcga gcacgccgta catgcgacgc agcttctgct tctcgcgcag ctgcaaacca
     5761 taatcggaca ggcgctgacg gcgctcgccc ttgatgccgc ccggcggcac cgtgaacttg
     5821 cacttgctct cgagcggctt cacgccgctc ttgagaaaga gatccgtgcc ctcgcgccgg
     5881 gcgagcttgc acttcggacc tacgtaacgc gccatacctt aacttcctgg ctgaaaggcg
     5941 cctgcggcgc tgtcagcggc ttgctggtgt gcaaatccgg tacgcgcgcc aagaacgtga
     6001 tccttgcgcg cgaaagcttg ccggtcatgc aacagggact catgcactaa tgcctcaaac
     6061 tctgcgcttc ttgggcggtc ggcagccgtt gtgcggaatc ggcgtgacat ccgcgatgct
     6121 cgtgatcttc agcccacagt tgttgagggc gcgcaccgcc gactcgcgtc ccgggccggg
     6181 gccgcccact cgcacctcga cattcttcac gccatgctcc tgcgccgcga cgccagcctt
     6241 ctcggcggcg acctgggcgg caaacggcgt gctcttgcgc gagccgcgga agccacaccc
     6301 gccggaggtc gcccaggaca gcgtgttgcc ctggcggtcc gtgatcgtga tgatcgtgtt
     6361 gttgaaggag gcatgcacgt gcgcgatcgc atccagcacg ttcttgcggg ccttcttcgg
     6421 cttcttggca gcgctcgccg cccctgcggc agcgccgccc gatttctgat cagccatctg
     6481 gcgtcaaccc tcgttatgcc ttgcccggtg gggcattgag cttcacggcc tgcttgcgcg
     6541 gacccttgcg cgtgcgcgcg ttcgtgcgtg tgcgctggcc gcgaaccggc aagccgcggc
     6601 gatggcggat gccccggtac gagcccagat ccatcagtcg cttgatgttc atggacactt
     6661 cgcggcgcag gtcgccctcg acggcgaact tcgcaatctg cgagcggatg gcgttcacct
     6721 ccggctcggt cagatccttc accttggtgg tcggatcgac gcctgcggcc tcgcaggcca
     6781 tccgcgcacg cgcgcgaccc acgccgaaga tgtgcgtgag tccgatccac acatgcttgt
     6841 tcatcggaat gttgatgccg gctatgcgcg ccatgctgct ctgccttcgc cgctgcggaa
     6901 cctgaaaaag cgcggcattt tacgtgtata ccgagtcatt accaaatcga gggccattac
     6961 cggatcaacc ctggcgctgc ttgtggcgcg ggtcgctgca gatcacatag accacgcgcc
     7021 ggcggcgcac gatcttgcag ttcttgcaga tcttcttcac cgacggacgt actttcattt
     7081 cgtgcgctcc ttcctcagcc gcccggcccg gtacgaccgt agccgagcag gttcgccttc
     7141 ttgagcaggc ccgggtagtg gtgtgacatc aggtgagcct gcagctgcgc catgaagtcc
     7201 atcacgacca cgacaacgat cagcagcgag gtgccaccga agtggaacgg cacgccctgc
     7261 gatgacacca ggatctccgg caacagacag acggccgcca cgtacagcgc accccagagg
     7321 gtgagacggg tgagcacctt gtcgatgtac tctgccgttt gctggcccgg acgtatcccc
     7381 gggatgaaag cgcctgactt cttcaggttg tcggcggtat cgcgcgcgtt gaacaccagg
     7441 gccgtgtaga agaagcagaa gaaaacgatc agcagcgcgt agatcaccag gtgcaggggc
     7501 tggccatagc cgagtgcctg cgacacgtcc tgcatgaaga tggcgaacgg cccctgcgag
     7561 cctgagcccg cgaagcttgc gagcgtcgcc ggaaacagca gcaggctcga tgcaaagatc
     7621 ggcggaatca cgcccgacat gttgagcttg aagggcagat ggctgctctg gccggcatac
     7681 atgcgccgcc ccacctggcg cttcgcgtag ttcaccgcga tgcggcgctg ggcgcgctcc
     7741 acgaacacga cgaacgccgt cgtcccgagc acgagaatga tcagcagcag cgcgaacggt
     7801 ccgctcatct caccggtgtt gaccgcctcg gcggtgttgc cgatggcccc aggcaggccc
     7861 gccacgatcc ccgcgaggat gatcatcgag atcccgttgc cgatgccgcg ctcggtgatc
     7921 tgctcaccga gccacatgag aaacatcgtt cccgtggtca tcgtcaccgt cgcggtgaag
     7981 aggaactgcc agccccccac cagcgtcagg ccgcctttga cgagcgcacc ggcggcggcg
     8041 aacgactgaa aggctgccag caccaccgtt ccatagcgcg tgtactcggt aatcttgcgc
     8101 cgccccgact cgccctcctt gcgcagctcc tgcagctgcg gaatcaccat cgacatcatc
     8161 tggatgatga tggaggcgga gatatagggc atgacgccca tcgcgaagat ggaaagccgc
     8221 gacagcgctc caccggagaa catgttcacg atccctagga tcgtgttgga ctgatccgag
     8281 aagaagcgcg ccacctcgct cggattgatg cccggtacgg ggatgaacgt gccgatgcgg
     8341 tagacgacca gcgcggcgat caggaaaaac aggcgcccgc gaacttcccc gaaacgggca
     8401 gcctcgccga gtgcggcagc gggattgctg aaggtggaat tggccacgcg tgtgtcgctc
     8461 taggtgggga ttaccggtct cgtttgcaag ctcaagcctt ctccgcgccc ccggcaccgc
     8521 cgggagttcc gccggtctcg ccgccgcctt ttggcccgcc agccttggcg cccttgccgg
     8581 cgctcttggc ggccttggcc tggctttttg ccgcttgcgc atcggccttg gcctgctctt
     8641 gtgcctcggc tttcgctcgg gctaccgggt cgcgggcctt ggcgggctcg ctcttcacct
     8701 ccggctcctc gaccttgccg cccgcggcct cgatcgcggc gcgcgcccct ttcgtggcgg
     8761 cgacgccctt cagcgtcagg gcgtgcttga tctcccccga cagaatgacc tttgcccgct
     8821 ccgtagccgc cggcacgatg ccggccttct tgagggcctc gaggtcgacc accccttcca
     8881 gcccgctgag ctcattcagg cgcacctcgg cccgcaggac cgccatgtgc gaacggaagc
     8941 cgaacttcgg caagcggcga tcgagcgggg tctggccacc ctcgaacccg accttgtggt
     9001 agccgccctt tcgggctcgc tgtcccttca cgccacggcc gcacgttttg ccctggccgg
     9061 ccgaggcacc gcggcccacg cgcaggcggg gacgcttcga tccgggagca ggttttaatg
     9121 agttcagtcg catctcgttc atcctaagca gccctgggcc gctacccagc gcggccgccc
     9181 gaccggtcca ctttcaacag gtgcatcgcg ccgtggatca ttccgcggtt ctcgggagtg
     9241 tcggccaccg tgaccgtctg gccaatgcgg cgcagcccga ggccgcgcgc gcaggcggcg
     9301 atacttgcga gctgaccgtg caggctcttg atcagcgtta ccttcacttg ggccttgtcc
     9361 gccatggcat tcaaccgatg atctcttcga gggtcttgtt gcgcttcgcc gcgatgtcgt
     9421 cgggagagcg gacgcttccc agcgccttga tcgtggcgcg taccacgttg atcgggttgc
     9481 gcgatccgta gctcttggcg aggacgttgc gcacgcctgc acattcgagc accgcacgca
     9541 taccgcctcc ggcgatgaca ccggtgccgt cggatgcagg ttgcatgtag acgcgcgtgg
     9601 cgccgtgcac gcctttgacc gcgtaatgca gtgagtcgtt gcgcagggcg acattgatga
     9661 ggttcttgcg cgcctgcgcc atggccttgg agatcgccac gggcacttcg cgagccttgc
     9721 caaagccgta gccgactcgc cccgagccat cgcccacgac agtgagtgcc gtgaagccga
     9781 actggcggcc gcccttgacg acctttgcgg tccggttcac ggcgacgagc ttctcgacga
     9841 actcgtcccc cgactgcgct ttttccggtc ttgccatgtc tgtatcctaa aattcgaggc
     9901 ccgcttcacg ggccgcctcc gcgagcgctt tgatccgccc gtggtactgg aagccggcgc
     9961 gatcgaacgc cactttgccg acgccggccg ccttggtccg ttcggcgatg acgcggccca
    10021 ccgccttggc cgcctcgacg ttacctgtgc ctttcaagcc ctgtcgcacg ccttcctgca
    10081 gcgttgaggc ggtggcaatc acccgggctc catctgctgc agtcacctgc gcgtagatat
    10141 gacgcggagt gcggtgcacg gtgaggcggg ccacatcgag ctggcggatg tgctcgcgcg
    10201 tcttcaccgc gcgccgaagc cgccgttgct tcttcgtgat gatcatttct tcttgccctc
    10261 cttgagaatg atctgctcac cggtgtagcg cacgcccttt cccttgtagg gctccggggg
    10321 tcgcagatcg cggatctcgg cagccacctg gcctaccttc tgccgatcga tgccccggac
    10381 gatgatctcg gtctggctgg gcgcctcgat cgtgatgccc tcgggaatgg cgaagttcac
    10441 cgggtgggaa aacccgagcg tcagattcag gctcttgccc tgtacctgcg cgcgaaagcc
    10501 tacccctacg agctcgagct tgcgctcgta gcccttggtg acgccgcgaa ccatgttggc
    10561 gaggtgcgcc cgggtagcgc ccgcctgcgt gcgcgccagt cccggatcgt catacttgat
    10621 ctgtagcttc ttgtcggtct gcacgaccga cacgccgcgc ccgagcggca cgctgagcga
    10681 gcccttgctg cctttgatgg tcaccgagtc ggccgagacg gtcgccgtca cgccctgcgg
    10741 cagatcgacc ggtgccttcg cgattctgct catgggcgta gccccgaaga ttcgcagatc
    10801 aggcgacgat acataggacc tccccgccct ggccgatcga gcgggcctgt ctgtccgtca
    10861 tcactccctt gggggtggag acgatcacgg tccccattcc accgagaatc ttcggcagct
    10921 ggtccttgcc gcgatagatc cgcagtcccg ggcggctgac acgctcgatg cggtcgatga
    10981 caggtcggcc ctcgtaatac ttcaggccga tggtcagcgt cggcttgccg ccatcgctct
    11041 cggtgcggaa atccgcgatg tagccttctt ccttgaggac cttgacgatg gccgtcttga
    11101 gcctcgaggc ggcgagctgc acttcactct tgcccgcggt ctggccgttg cggatgcggg
    11161 tcagcagatc ggcgatggga tcggtcatgc tcatgcggcg ttcctcacca gctcgccttc
    11221 ctgaggcccg gaatctctcc acggctcgcc acttcccgga tcttcacccg ggagaggccg
    11281 aacttgcggt ataccccgcg cgagcgaccc gtgatcgcac agcgtttgcg ctgccgcgac
    11341 ggactcgcat cgcgtggcag cgattgcagc ttgctctgcg cagccatacg ctcctcgggc
    11401 gaggtgcggg ggctgcggat cagctccttc agctgcgccc gctgagcggc atactgcttt
    11461 actattttgg cgcgacgtct ctcgcgctgg accatgctgg tcttcgccat gtcttatcct
    11521 atgggctcaa acgacctgcg gccgtttgag cgagccatcc tgttgataca tccacacccg
    11581 cgggcaaaaa ccgctgcttt tgtccgcttg atttcgtgcc ggtcgttcct catgtgcctg
    11641 accttacttc ctgaacggga agttgaaagc ctcgagcagc gcccggccat gacggtcgtc
    11701 cgtggccgtt gtcgtgatcg tgatgtccat gccccggatc tgatccacct ggtcgtactg
    11761 gatctccggg aagatgatct gctccttcac gcccaggctg tagttgccct ggccgtcgaa
    11821 tgcgcgcggc gagacgccgc ggaaatcccg gatgcgcggc atcgcgatgt tgatcagccg
    11881 gtccaggaac tcatacatcc gcactccgcg cagggtcacc ttggcaccca ctgccagccc
    11941 cgcgcgcagc ttgaaggaag caatcgcttt gcgggacttg gtgatcagcg gtttctgtcc
    12001 ggtgatcttg gtgagatcgg tgatggcggc atccatgacc ttacgatcag cgacagcctc
    12061 gccgacgccc atgttcaccg tgatcttgct gatgcgcggc acctgcatcg ggttgccgat
    12121 cttgagatcc gacctcagcc ggggcacgac ggtctcgcgg taatattgct gcagcctggc
    12181 catgtttgat tctgccgcga cgccctcagg catcgagcat ctctccgttg gatttgaaga
    12241 agcgcacctt cttgccgtcc gcaagcgtct tgaatccgat gcggtcggcc ttcttcgcgc
    12301 ctggattcca gatcgccacc ttcgacaccg gcagcggcat ttccttctcg atgatgccgc
    12361 cctgccggtt cgcctgcgga ttgggcttct ggtggcgctt cacccggttc acgctctcga
    12421 ccagcacgcg gccgtcgccg agaacctgga ccacggcgcc gcgccgcccc ttgtcacgtc
    12481 cggccagcac cacaacctga tcgcccttgc gaattttctt catgagtacg aacccctcgg
    12541 cgctcacagc acttctggcg cgagcgagat gatcttcatg aactgcacgt tgcgcagctc
    12601 gcgcgtgacg gggccgaaga tgcgggtgcc gatgggctcc agcttgttgg tgagcagtac
    12661 cgctgcatta ccgtcgaagc ggatcaacga gccatccggc cggcgcactc cgtgcgtggt
    12721 acggacgacc accgcgtcat acacctcgcc tttcttcacc ttgccgcgtg gaatcgcatc
    12781 cttcacgctg accttgatga catccccgac ggtcgcatag cgacgccgcg tgcccccgag
    12841 caccttgatg cacatcaggg tgcgtgcgcc gctgttgtcg gcagcattga gcattgtgcg
    12901 caactgaatc atcgctgctc ctcagcgccc cgcggccttc tcgaggacct cgaccagctt
    12961 ccacgacttg cgccgcgaca gtggccggca cggagcgatc
//
LOCUS       DASRHN010000258         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1408924, whole genome shotgun sequence.
ACCESSION   DASRHN010000258 DASRHN010000000
VERSION     DASRHN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408924"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VFB37_10270"
     CDS             <1..1666
                     /locus_tag="VFB37_10270"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZO22874.1"
                     /translation="EAEVVSQQLMLRAGLIRRLAAGLYSWLPLGLRTLRKVETIIREE
                     MNRAGALEMLMPMVIPGELWQESGRWKEYGPELLRFKDRHERDFCLGPTHEEVITDIV
                     RREIKSYRQLPANFYQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHADERSLQEGYRA
                     MYEAYSRIFTRTGLEFRAVRADSGAIGGDVSQEFHVLASSGEDAIVFSDGDDYAANLE
                     AAAALAPAEPRRAPAEALRKVPTPGARTIEELARFLSVAPSRTLKTLLVDGTEQDDVV
                     ALVLRGDHELNAIKAQKLQGVASPLRMSSAERIRAATGTEPGYLGPLGFRGRLYADHG
                     ALALADFVCGANEQDMHYVGANWDRDIQGAVPADLRKVVEGDPSPSGRGRLKLARGIE
                     VGHIFQLGCKYSEPMKALVLDENGREVIMHMGCYGIGVTRIVAAAIEQNHDERGIIWP
                     DPIAPFQVVLVPIGLQKSAQVREAADRLYAQLSAAGIEVLYDDRDARPGVKFADAELL
                     GIPHRLVVAERGLQAGMFEYRHRRETESREFPAADALAFIRSRLAV"
     gene            1629..2273
                     /locus_tag="VFB37_10275"
     CDS             1629..2273
                     /locus_tag="VFB37_10275"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZO22875.1"
                     /translation="MHWLSSARGWLSERLLRGFLATALLLTASAHADQQRDPELRAVV
                     ARAISQAECFTDHYDSAVWYTLMEPRLRPTVKERDERLEILKQVYCETHRAGESRLPP
                     GLVMAVMAVESRFDRWAVSSSGAVGLMQVMPFWPTQLGVRRYELIHIGPNIHMGCAIL
                     RYYLRYEHNDVRRALARYNGSVGRRDYPDKVLTQWSKWGGADDLGFPSAERVHH"
     gene            complement(2203..3966)
                     /gene="ggt"
                     /locus_tag="VFB37_10280"
     CDS             complement(2203..3966)
                     /gene="ggt"
                     /locus_tag="VFB37_10280"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZO22876.1"
                     /translation="MLSLVPVVAHAGQPAPARPAAQQAVQHTAIVAAANPLAADAGVE
                     ILRRGGNAADAAVAIQAMLGLVEPQSSGVGGGAFMLYYDAASGVVSALDGREKAPAGA
                     TPDMFLDEDGKPMSYLQAVRTGRSTGVPGAIAMLSMAHARLGELPWRELFAPAIRTAT
                     EGFRVSPRLAGYLAPDFVLPATAEVRSLFARADGQPLQAGDLFRNPQYAHTLRRIAAE
                     GPRALYEGSIAAQIVARIHEQPTPGTMTLRDLSAYRADWERPLCRPYREYSVCVPPPP
                     SSGVNLLQLLTMLDDTDIAKRGPTDPQAWFLFAQASRLMYADRDRYVADPAFIPVPVS
                     KLLDPSYLSERARLIGEHAGPAPEPGQLAGVIRAPDATREAAGTSHFVVMDSAGNVAS
                     MTTTVESVFGSGRTVEGFVLNNQLTDFSYRPVHEHGPVANAVQGGKRPRSSMAPIIVL
                     DHEGHFVAALGSPGGSSILEYSAKTVVGVLAWHLTMQQAIDLPNLIARGEDFTGEMRR
                     FSPSIVAGLRDRGIDLKEGRAENSGLHGLMRTPDGSYEGGADPRREGVVRLVPPQSLS
                     DAHAPRKGTRDHQRLPTSTTG"
     gene            complement(4045..4320)
                     /locus_tag="VFB37_10285"
     CDS             complement(4045..4320)
                     /locus_tag="VFB37_10285"
                     /inference="COORDINATES: protein motif:HMM:NF011022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZO22877.1"
                     /translation="MVCRKCLVEGRVQGVFYRATAARRAQQLAIVGYARNLPDGRVEV
                     LACGDEMAVQAFVKWLWIGSSASRVTSVEVEEAPLDAGPLPSGFATS"
     gene            4539..5135
                     /gene="wrbA"
                     /locus_tag="VFB37_10290"
     CDS             4539..5135
                     /gene="wrbA"
                     /locus_tag="VFB37_10290"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HZO22878.1"
                     /translation="MTEILVLYYSRKGATAELARHVCRGIESVPEVSAKLRTVPPVFA
                     ESEGPARPVPASGPPYAALEDLRRADGLVLGSPTRFGNMAAPLKYFLDSSGALWLSGA
                     LAGKPAGVFTSTQTMHGGQETTLLSMMMPLIHHGMYLVGLPYTERALNQTRGGGSPYG
                     ASHVSGPQDDPALADDEKALAQALGRRVATLAAKLRSD"
     gene            complement(5146..5832)
                     /gene="hda"
                     /locus_tag="VFB37_10295"
     CDS             complement(5146..5832)
                     /gene="hda"
                     /locus_tag="VFB37_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HZO22879.1"
                     /translation="MQQLPLGVRMVDRARFGSFLPARNGEALEHAQRTAAGAASGVSW
                     FCGPPGAGKTHLLQAICCSASESETAGYFPLGELQRLGPGVLEGLAQLGCVCLDDVQG
                     LAGELSWERAVFGLLREIEELGRRLVLAAPSPPALLNWKLPDLGSRLAAAAIFQLRVL
                     DEAEQREALQLRARMRGVELPEETSRWLQRRFPRDMRTLYELLDTLDEAALVAQRRLT
                     VPFIRSVLRE"
     gene            complement(5844..>6381)
                     /locus_tag="VFB37_10300"
     CDS             complement(5844..>6381)
                     /locus_tag="VFB37_10300"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZO22880.1"
                     /translation="NLICLLRIALVWPIAAALHTRQHFEALALFVAAGFSDGLDGYLA
                     KRFNWMSDLGKVLDPLADKLLLVVVFVEGAWLELIPWWLSAAVVARDVFIGLGALIFR
                     AWFGPLHGRPTVFSKINTGMQLLYLTAVMVSAALSFPPREVVAALGFIVLATTVISGL
                     NYLTIFTRRAWAQAAGAS"
BASE COUNT         1153 a         2107 c         2093 g         1028 t
ORIGIN      
        1 ggaagccgag gtggtgagcc agcagctcat gctgcgagcc gggctcatca ggcgcctggc
       61 ggcaggcctg tacagctggt tgccgctcgg gctgcgcacg ctgcgcaagg tcgagaccat
      121 cattcgcgag gagatgaacc gcgctggagc gctggaaatg ctgatgccga tggtgatccc
      181 cggcgagctg tggcaggaat cgggccgctg gaaggaatac ggaccggagc tgctgcgctt
      241 caaggaccgc cacgagcgcg acttctgcct gggtccgacc cacgaggagg tcatcaccga
      301 tatcgtccga cgcgagatca agagctatcg gcagctgccg gccaacttct atcagatcca
      361 gaccaagttc cgggatgaga tacggccccg ctttggcgtg atgcgtgcgc gcgaattcat
      421 catgaaggac gcgtactcct ttcacgccga cgagcgctcg ttgcaggagg gctatcgggc
      481 catgtacgag gcctacagcc ggatcttcac ccgcaccggg ctcgaatttc gcgcagtgcg
      541 tgccgactcg ggcgccatcg gcggtgatgt gtcgcaggag ttccacgtct tggcgagctc
      601 cggggaggac gccatcgttt tctccgacgg cgacgattac gctgccaatc tcgaagccgc
      661 tgctgcgctg gcgcccgcgg agcctcggcg agcgcctgcc gaggcgctgc gaaaggtccc
      721 tacccccggt gcccgcacga tcgaggagtt ggcgcgcttt ctcagcgttg cgccctcgcg
      781 gaccctcaag actctgctgg tcgatggcac cgagcaggat gatgtcgtcg cattggtact
      841 gcgtggtgat cacgagctca acgccatcaa ggcgcagaag ctgcaagggg tcgcctcgcc
      901 gctgcgcatg tccagcgccg agcgcatccg cgctgccacc ggcaccgaac ccggatatct
      961 gggaccgctc ggctttcgcg gccgcctcta cgcagaccat ggcgcgctcg ctctggctga
     1021 cttcgtctgt ggggccaacg agcaggacat gcactatgtc ggggccaact gggatcgcga
     1081 catccagggc gcggtcccgg ccgaccttcg caaggtcgtc gagggtgacc cgagtcccag
     1141 cgggcggggg cgcctcaagc tcgcccgggg catcgaggtg ggccatatct tccagctcgg
     1201 ttgcaagtac agcgagccca tgaaggctct ggtgctcgat gaaaacggcc gggaagtgat
     1261 catgcacatg gggtgttatg gcatcggtgt gacacgcatc gtcgccgccg cgatcgagca
     1321 gaatcacgat gagcggggca tcatctggcc ggatcccatt gccccgttcc aggtggtgct
     1381 ggtgccgatt ggtctacaga aatccgccca ggtgcgcgag gccgccgatc ggctgtacgc
     1441 gcagctcagc gcagcgggaa tcgaggtgct gtacgacgac cgtgacgccc gcccgggggt
     1501 caaatttgcg gatgccgaat tgctcggcat tccccaccgt ctggtggtgg ccgagcgtgg
     1561 gctgcaggcg ggcatgttcg agtatcgcca ccgccgcgaa accgagagca gggaatttcc
     1621 cgccgccgat gcactggctt tcatccgctc gcggctggct gtctgagcgc ctcctgcggg
     1681 gcttcctcgc gaccgccctg ctcctgacgg cgagtgcgca cgccgatcag cagcgcgatc
     1741 cggagctgcg agccgtggtt gctcgggcca tttcacaagc ggaatgtttc acggaccact
     1801 acgactccgc ggtctggtac acgctgatgg agccgcgatt gaggccaacg gtcaaggaac
     1861 gtgatgaacg tctcgagatc ctgaagcagg tctactgtga aacccaccgc gccggtgaga
     1921 gccggctgcc gccgggactc gtcatggcgg tgatggccgt ggagagccgt ttcgaccgct
     1981 gggcggtctc ctcctccgga gcggttggac tcatgcaggt gatgcccttc tggcccacgc
     2041 agctcggtgt gcgacgttac gagctgattc atatcggccc caacattcac atggggtgcg
     2101 ccatcctgcg ctattacctg cgctatgagc acaatgacgt gcgccgagcg ctcgcgcgct
     2161 acaacggcag cgtcggacgc cgcgactatc ctgacaaggt gcttacccag tggtcgaagt
     2221 ggggaggcgc tgatgatctc gggttccctt ccgcggagcg tgtgcatcac tgagcgactg
     2281 cggcggaacc aacctgacaa cgccctcgcg acgcggatcc gcgcctccct cgtagctgcc
     2341 atcgggtgtg cgcatcagac cgtgaaggcc cgagttctcc gcgcgacctt ccttcagatc
     2401 gatgccccgg tcgcgcagcc cggcgacgat cgacggcgag aaccggcgca tctctccagt
     2461 aaagtcctcg ccacgagcga tcaggttcgg cagatcgatc gcctgttgca tcgtcaggtg
     2521 ccaggcgagc acgccgacca cggtcttggc gctgtactcg agtatcgagg agccgccggg
     2581 tgagccgagg gctgcgacga agtgtccctc gtgatcgagc acgatgatgg gtgccatgga
     2641 agagcgcggc cgcttaccgc cctgaaccgc attggcaacg ggcccgtgct catgcacggg
     2701 ccgatacgag aagtccgtca gctggttgtt gagtacgaag ccctcaacgg tgcggccgga
     2761 gccgaagact gactccaccg tggtcgtcat cgatgcgacg tttccggcgg agtccatcac
     2821 gacgaaatga ctcgtgccgg ccgcttcccg ggtcgcatcc ggcgcgcgga tcacaccggc
     2881 gagctggcct ggctccgggg ccggtccggc gtgctccccg atgagccgtg cgcgctcgct
     2941 caggtaggag ggatcgagga gcttgctcac gggtaccggg atgaaggccg gatcggcaac
     3001 gtaacgatcg cgatcggcgt acatgagccg gctcgcctgg gcgaacagga accatgcctg
     3061 cgggtcggta ggcccgcgct tggcgatatc ggtgtcatcg agcatcgtca ggagctgcaa
     3121 caggttgact ccgctggagg gcggcggcgg cacgcaaacg gagtactccc ggtagggacg
     3181 gcacaaaggc cgttcccaat cggctcgata ggcggacaga tcgcgcaagg tcatggtgcc
     3241 gggagtcggc tgctcgtgga tccgtgccac gatctgcgcg gcgatgctgc cttcgtagag
     3301 cgcgcgcggg ccctcggcgg caattcgccg gagcgtatgc gcatattgcg ggtttcgaaa
     3361 caggtcacct gcctgcagcg gctggccgtc cgctcgtgca aagagcgatc ggacctcggc
     3421 cgtggccgga agcacgaagt ccggtgccag atagcccgcc agcctgggcg agacccgaaa
     3481 gccctcggtc gccgtacgga tcgccggggc gaataattcg cgccacggca gctcccccag
     3541 ccgcgcgtga gccatggata gcatggcgat ggcgcccggg acgcccgtgg atcgtcccgt
     3601 acggacggcc tgcagatagg acattggctt gccgtcctca tcgaggaaca tgtccggggt
     3661 ggcacccgcc ggggcctttt cgcggccgtc gagtgccgag acgacgccgc tcgccgcgtc
     3721 gtaatacaac atgaacgcac ccccgccgac ccctgagctc tgtggctcga ccagtcccaa
     3781 catggcctgg atcgccacgg ccgcgtcggc cgcgttcccg ccccggcgca ggatctccac
     3841 gccggcatcc gccgccaatg ggttggcagc ggccacaatg gcagtgtgtt ggacggcctg
     3901 ctgtgcagcc ggccgcgcag gcgctggctg ccccgcatgc gccaccacgg gcacaagtga
     3961 cagcatgacc aacacagatg acacgcagtg tcgcagtcgc ggatcaagca tgattgacct
     4021 caaaggaagc cgcggcggcg cagctcaact cgttgcgaat ccggagggca gcgggccggc
     4081 atcgagcgga gcttcttcca cttcgaccga ggtcaccctg gaggcggacg agccgatcca
     4141 gagccacttc acgaacgcct gcacggccat ctcatccccg caggcgagca cttccacccg
     4201 gccgtccggc agattacggg catatccgac gattgcgagc tgctgcgcac gccgcgcggc
     4261 cgtagcgcgg tagaaaactc cctgcacacg gccttccacc agacacttcc tgcagaccac
     4321 gccagctcct catcgcctgt gagcgaaata ttagcgcaag acagcgcttt tgcccggctg
     4381 ccggggacgc cgccgaaaac cagaatggac gacgggggtc tgcaacccgg gatggatccg
     4441 atatggccgg acacggttcc tctgtggcaa cgtatccgca gcgcgcgcag cgttacccta
     4501 tctgcgcgcg accgtgcccc ggggcgacag aggctctgat gacggagata ctggttctct
     4561 actactcgcg caagggcgcc acggccgagc tggcgcggca tgtctgccgc ggcatcgaga
     4621 gtgtgcccga ggtgagtgcg aagctacgga ccgtgccacc ggtgtttgcc gaaagcgagg
     4681 gcccggcgcg accggtaccc gcgagcgggc caccctacgc agcgctcgag gatctgcgcc
     4741 gcgcggatgg tctcgttctg ggcagtccga cccgcttcgg caacatggcg gcgccgctca
     4801 aatacttcct cgatagcagc ggggccctgt ggctctccgg ggccctggct ggaaaacctg
     4861 ccggcgtctt cacctccacc cagacgatgc acggcggcca ggagaccact ttgctgtcca
     4921 tgatgatgcc gttgatccat cacggcatgt atctggtggg gctgccgtac accgagcgtg
     4981 cgctcaatca gacccgtggc ggaggctcac cctatggcgc ctcgcatgtc tcagggcccc
     5041 aggacgatcc ggcgctggcg gacgatgaga aggcactcgc ccaggcgctc ggccgcaggg
     5101 tggctacgct ggcggcaaaa ttgcgctcgg actgacaagg gcaggctatt cccgcagtac
     5161 cgaccggatg aagggcacgg tcaggcggcg ctgggcgacc agggcggcct catccaatgt
     5221 atccaacagt tcatagagcg tacgcatgtc ccgcggaaag cgccgctgca gccagcgcga
     5281 ggtttcctcc ggaagctcga cgccgcgcat gcgggctcgc aactggaggg cctcgcgctg
     5341 ctcggcctca tccaggaccc gcagctgaaa gatggccgcc gccgccagac gtgagcccaa
     5401 atccggaagc ttccagttca gcagtgcagg cggtgaaggc gcggccagca ccagccgccg
     5461 gcccagctcc tcgatctcgc gcagcagtcc gaacacggct cgctcccagg aaagctcacc
     5521 agccagccct tgcacatcat ccaggcagac gcacccgagc tgggccagac cctccagcac
     5581 ccctgggccg agccgctgca actcgccgag cggaaagtag cctgctgtct cggactcact
     5641 ggcgctgcag cagatcgctt gcagcaggtg tgtttttccc gcccctgggg ggccacagaa
     5701 ccagctgacg ccgctcgccg caccggcggc tgttcgctgc gcatgctcca gcgcttcccc
     5761 attgcgtgcc ggcaggaagc tgccgaagcg cgcgcgatcc accatccgta cgccgagggg
     5821 aagctgttgc acgaacaatc gcctcaggag gcgccagcgg cctgggccca tgcccggcgt
     5881 gtgaagatcg tgagatagtt cagcccggaa attacggtcg tggcgagcac gatgaacccg
     5941 agtgccgcca cgacttctcg tggcgggaag ctcaaggccg cactgaccat gacggccgtc
     6001 agataaagga gttgcatgcc cgtgttgatc ttactgaata cggtcggacg tccgtgcagc
     6061 ggtccgaacc aggcgcgaaa gatcagcgcg cccagaccga tgaatacatc gcgggccacg
     6121 actgcagcac taagccacca ggggatcagc tccagccacg cgccctcgac gaaaaccacg
     6181 acgagcagca gcttgtcggc caacggatcg agcaccttgc ccagatcgga catccagttg
     6241 aagcgcttcg ccagatagcc atccaggccg tcggaaaacc ccgcggccac gaacaacgcg
     6301 agcgcctcga aatgctgccg cgtatgcaaa gctgcggcga tcggccacac caacgcaatg
     6361 cgcagcaggc agatcaggtt a
//
LOCUS       DASRHN010000259         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2067372, whole genome shotgun sequence.
ACCESSION   DASRHN010000259 DASRHN010000000
VERSION     DASRHN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067372"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFB37_10305"
     CDS             complement(<1..732)
                     /locus_tag="VFB37_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZO22881.1"
                     /translation="MAAALLQAAAPVHAQDPQSDSRDEDTSSSKAAPKKPGEAGPVVI
                     EAKRWLFNRETRYAHSLPEVDGATITVTKKTSVVKLDEQPTIIDNNQRELFDRLPGIV
                     LAEQQNPTQLNLSYRGLGNPQESEFILLMQDGIPLELDWIGYPTLYYLPVPETVSAVQ
                     MIRGGSGLLYGPEPQPVINFVSRAPATQPLAGTTEQVGGDQGLFSSFNAVSGTLGPWD
                     YLADYSHRHSSGQRMNGDYTLNSGDL"
     gene            complement(836..>2479)
                     /locus_tag="VFB37_10310"
     CDS             complement(836..>2479)
                     /locus_tag="VFB37_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22882.1"
                     /translation="QTWERISPDLTSNDPQKQKQEQSGGVTVDNSAAEMHTTIYSISE
                     STLTAGLIWVGTDDGNLQLTRDGGKHWDNLVHNVHGLPPNSWVSWVQAGLYAPGTAYA
                     AFDRHTFGDMAPYVYVTTDYGKTWTPLLTAHDAHGVRGYAHVIKEDPVDSDLLFLGTE
                     FGLWISADRGAHWAQFKGGHIPDVAVRDLAIQSRDNDLVLATHGRGIWIVDDITPLRH
                     LTPELLAKDAAFVSARPVQQRIEANGGWPLGAAAFIGDDPPAGAQITYFQRTRHIFGK
                     LKIEVLDAQGHVVDELPASTRRGLNRVAWTMHLSPPHVPPAVQLAQAGTQGPRVLPGT
                     YTVRMEKNGQTYTTSISVGLDKRVKWTAADRQAQFEAATKVEALFNDESTLFGRIAGL
                     RQQIGELTKDHKPAQSVAHRLSEFDAKLDGLRKQIVATTEGGAITGEERLREHTDQLY
                     GAITSWEGSPSGYQLENITALRGQLGDIENAFNHLTSSELPVLNKLLQSNGMGSLNVP
                     QTAALEDAETGGAGGRAGLRFDPDAAHATEMPANLRLWN"
BASE COUNT          408 a          783 c          799 g          489 t
ORIGIN      
        1 cagatcgccc gagttgagcg tgtagtcgcc attcatgcgc tgcccgctcg agtgtcgatg
       61 cgaatagtcc gccaggtagt cccaggggcc gagggtcccg gagaccgcat tgaagctcga
      121 gaacagtccc tggtccccgc cgacctgctc ggtcgtgccg gcaagaggct gggtcgccgg
      181 cgcacgcgag acgaagttga tcacgggctg aggctccgga ccgtacagca gtcccgagcc
      241 gccgcggatc atctgcactg cgctcacagt ctcgggaacc ggcagataat agagtgtcgg
      301 atagccgatc cagtcgagct cgagcggaat cccatcctgc atcagcagga tgaactcgct
      361 ctcctgcgga ttgccgagac cccgatagga gagattgagc tgggtcggat tctgttgctc
      421 cgcgagcacg attccgggca ggcggtcgaa cagctctcgc tgattgttgt cgatgatcgt
      481 cggttgctca tcgagcttca ccacgctcgt cttcttggtg acggtgatgg tcgcgccatc
      541 gacttccggc agggaatgcg cgtagcgggt ctcgcgattg aacagccagc gtttggcctc
      601 gatgaccacc ggccccgcct cgcccggttt cttgggcgcg gctttgctgc tcgaggtgtc
      661 ctcgtcgcgc gaatccgact gtggatcctg ggcgtgaacg ggagctgctg cctgcagcaa
      721 agcagcggcc acgcagaggc tcaggcagcc gggctttgcc cgccaaagcg cgcggcccgt
      781 cgaatgtctt gtctgatgtt cggtttgtga ctcgtggctg accgccgccg gcatgtcaat
      841 tccaaagccg caggttggct ggcatttcgg tggcgtgcgc cgcgtccgga tcgaaccgga
      901 gaccggctcg gccgccggca ccgccagtct cggcatcctc gagggctgcg gtttgaggga
      961 cgttgaggct ccccattccg ttgctctgca gcagcttgtt gagtacgggc agctcgctcg
     1021 aggtcaggtg attgaaggcg ttctcgatgt cgccgagctg cccgcgcagc gccgtgatgt
     1081 tctcgagctg gtagccggac ggggagcctt cccaggacgt gatggcacca taaagctgat
     1141 cggtatgctc gcgcaagcgc tcctcgccgg tgatggcgcc gccttcggtg gtcgcgacga
     1201 tctgcttgcg caggccatcg agcttggcgt cgaactcgct caggcgatgc gcgacgctct
     1261 gcgcgggttt gtggtccttg gtcagctccc cgatctgctg ccgcagccct gcgatccggc
     1321 cgaagagggt ggattcgtcg ttgaacagcg cttcgacctt cgtcgccgct tcaaactgag
     1381 cctgccgatc cgccgccgtc catttgacgc gtttgtccaa gcccacggaa atcgaggtgg
     1441 tgtaggtctg cccgttcttc tccatacgca cagtataggt acctggcagc acccgcggac
     1501 cctgcgtgcc cgcctgagcg agctgcactg caggcggcac gtgtggcgga ctcagatgca
     1561 tggtccaggc cacgcgattc aatccgcgcc gggtgctcgc gggcaactca tcgaccacgt
     1621 gcccctgtgc atccagcact tcgatcttga gctttccgaa aatatggcgt gtgcgctgga
     1681 aatacgtgat ctgcgcgcca gcgggcgggt cgtcgccgat gaacgcggcc gcacccagcg
     1741 gccagccgcc gttcgcctcg attcgctgct gcaccgggcg tgcggagacg aaagcggcat
     1801 cctttgcgag tagctccgga gtgagatgcc gcagaggggt gatgtcatcc acgatccaga
     1861 ttccgcggcc gtgcgtggcc agcaccagat cgttgtcgcg ggactggatt gcgaggtccc
     1921 gcacggcaac atccggaatg tggccgccct tgaactgcgc ccagtgagca ccgcgatccg
     1981 ccgagatcca caggccgaac tcggtaccga ggaacaaaag atcggaatcc accggatctt
     2041 ccttgatgac gtgcgcatag ccgcgcacgc catgcgcatc gtgcgccgtc agcagcggcg
     2101 tccaggtctt gccgtaatcc gtggtcacat acacgtatgg ggccatgtcg ccgaaagtgt
     2161 gccgatcgaa tgcggcatag gccgttccgg gcgcatagag gcccgcttgc acccagctca
     2221 cccaggagtt cggcggcagt ccgtgcacat tgtgcacgag gttgtcccag tgcttgccgc
     2281 catcgcgcgt cagctgcaga ttgccatcgt ccgtaccaac ccagatgagt ccggcagtga
     2341 gcgtcgattc gctgatcgag tagatagtcg tatgcatctc ggcggcagag ttgtcgaccg
     2401 tcactccgcc ggactgctcc tgcttctgct tctgcggatc attgctcgtc agatcgggcg
     2461 agatgcgctc ccaggtctg
//
LOCUS       DASRHN010000260         8175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_88293, whole genome shotgun sequence.
ACCESSION   DASRHN010000260 DASRHN010000000
VERSION     DASRHN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88293"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFB37_10315"
     CDS             complement(<1..468)
                     /locus_tag="VFB37_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO22883.1"
                     /translation="MSPHGNARLDRLQAYPFERLARLKAGLTPPRSLTPIAMSIGEPR
                     HAPPAFIVDKLRENSSLLDSYPTTAGVPELRAAAARWLERRFALPSRSVDPQTMILPV
                     NGTREALFSFVQAMVDGAGEPLVALPNPCYQIYEGAALLAGAEPFFLDTPDAAG"
     gene            complement(465..3164)
                     /gene="glnD"
                     /locus_tag="VFB37_10320"
     CDS             complement(465..3164)
                     /gene="glnD"
                     /locus_tag="VFB37_10320"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZO22884.1"
                     /translation="MVTPSPDLDLLEDTLPAPTRSAPWPLLVRLPSLLAQAQGSPAAY
                     RELLKQGHEELKARFLAEEPVEDLVHARASLIDLVLRHVWRMRQLEDADSWALVAVGG
                     YGRGELHPCSDIDIMLLAPAESGGREAAEGLVTFLWDIGLEVGHSVRTIAECVHEAKA
                     DVSVMTTLLEARLVDGSSALFEAMLAALSPEHVWPVREFFEAKVREQTERHLKANDTA
                     YNLEPNVKTGPGGLRDIQTIAWVAKRHFGSETLDGLATRGFLSMAELRRLKQAQAFLW
                     KVRFGLHALTGRREDRLLFDYQVRLALSFGYEDASYTLAVEQFMQRYYRTVMDVSLLN
                     ELLRQVFREAILSESEPPRPLNARFQVRNGSLEAVSADMFVRNPSALLEMFVLLQQSP
                     DINGVRASTMRAVARGLWLIDEEFRQNPRNHRLFLEIVRAPVGVTHELRRMNTYGVLG
                     RYIPAFGRIVGRMQYDLFHAYTVDAHTLFVVSNLRRFAISRYDHELPEASRIMQQLPR
                     TEIAYLAALFHDIAKGRGGDHSELGAVDAEAFCLEQGLSPYDARLVAWLVRNHLELSI
                     TAQKQDISDPQVINAFARKVGDEAHLDYLYVLTCADVRGTNPKLWNSWKASLFREFFD
                     RVKRALRRGLESPIDQEQLVRETQETARRLLHEQGVTGIDVDGAWRRFSAAYFLRHTP
                     DEVAWHTRLLAERDSGSDEPLVALDLRGVRGTTSVLIFARAERHGFSHTTAVLDQLGL
                     NIVDARITPTGDGFSLGLYHVLEDDGAPITDHDRHSEIEYALWRSVQQPEDSPFAVSR
                     RAPRQARMFNTPTHVAVSIDERNRRSVLELTASDRPGLLSDVGKVLMEERLQLHDAKI
                     MTVGERAEDVFFITDFEQRPLGAAAAQHLRDRLLHALDRRQAA"
     gene            complement(3176..3952)
                     /gene="map"
                     /locus_tag="VFB37_10325"
     CDS             complement(3176..3952)
                     /gene="map"
                     /locus_tag="VFB37_10325"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZO22885.1"
                     /translation="MHVTIKTPEEQEKMRIAGRLAAEVLDMIGEHVVPGVTTDELDRI
                     CHDHIVKVQHSIPANLNYRGFPKTICTSVNHVVCHGIPNDKRLKAGDIVNIDVTVIRD
                     GFHGDTSRMYFVGKPPPHAQRLAETCFEAMWRGIHTVRPGTRLGDIGHAIQSFVEERN
                     YSVVREYCGHGIGRVYHEDPQVLHYGEPGTGLELQRGMTFTVEPMVNAGKRHVRLLPD
                     GWTVITKDHSLSAQWEHTVLVTDSGYEVLTLGAANTPQAA"
     gene            4174..4848
                     /gene="rpsB"
                     /locus_tag="VFB37_10330"
                     /pseudo
     CDS             4174..4848
                     /gene="rpsB"
                     /locus_tag="VFB37_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            5224..6144
                     /gene="tsf"
                     /locus_tag="VFB37_10335"
     CDS             5224..6144
                     /gene="tsf"
                     /locus_tag="VFB37_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZO22886.1"
                     /translation="MNITADAVKQLRERTGAGMMECKRALVETQGDLDAAAELMRKQG
                     HAKADKKAARVAAEGVVIIEKSADGRTAAMAEVNCETDFVAREQDFRAFAQVVAARAL
                     ADRSPSLEALLAARAGNATETIEERRRALIAKIGENISVRRFVVLSSSDHLGAYVHGT
                     RIGALVVVTGGDAALAHDLAMHVAASNPRHLSAEDVPAEVVAKEREILTEQAQGEGKP
                     PQIVAKMVEGRLRKALSEITLTGQPFVKDPDVSIEKLLKSAGAQVTAYARFEVGAGIE
                     KKQDDFVAEVMAQVKSATPPAPGTTPPVKH"
     gene            6293..7021
                     /gene="pyrH"
                     /locus_tag="VFB37_10340"
     CDS             6293..7021
                     /gene="pyrH"
                     /locus_tag="VFB37_10340"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZO22887.1"
                     /translation="MSDAAKPRYSRILVKLSGEALLGSADYGVDPAVLKRIAGELQDI
                     IEMGVQVAVVIGGGNIFRGAGLARAGMDRVAADHMGMLATVMNSLAMQDALEALGMYA
                     RVMSAIRINEVCEDYIRRRAVRHLEKGRVVIFAAGTGNPFFTTDTAASLRAIEINADI
                     LLKATKVNGVYSDDPVRNPAAIRYATLTFDRVLADKLNVMDATAIVMCRDNRLPLRVF
                     NLNNSGDLTRIVRGEDVGTAVTFE"
     gene            7057..7614
                     /gene="frr"
                     /locus_tag="VFB37_10345"
     CDS             7057..7614
                     /gene="frr"
                     /locus_tag="VFB37_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZO22888.1"
                     /translation="MLEDLKKDARERMAKCVQTFQGDLKKLRTGRAHPSLIEHLRVDY
                     YGSEVPLQQVANIAVEDGRTLVVSPWEKSMVQPIEKAIHKSDLGLTPMSAGTVIRIPM
                     PPLTEERRRDITKVLRHDAENARVAVRNVRRDVMADIKEALKEKLVSQDDERRAETEV
                     QKLTDKHVADIDQLLAAKEKEVMQV"
     gene            7611..>8175
                     /gene="uppS"
                     /locus_tag="VFB37_10350"
     CDS             7611..>8175
                     /gene="uppS"
                     /locus_tag="VFB37_10350"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZO22889.1"
                     /translation="MTRLPRHVAITMDGNGRWAAARGVPRAAGHRAGLKPTRMVIQEC
                     ARRGVEALTLFAFSSENWKRPADEVSSLMALFVDSLDREIDELHARRVRVRFIGNRGR
                     LAMRLQTRIAEAEQRTAANPGLKLQIAVSYGGRWDIIQAAQKLAAQCSSGFIRAEDID
                     EPRFGATLELADVPDADLMIRTGGEQRI"
BASE COUNT         1499 a         2533 c         2793 g         1350 t
ORIGIN      
        1 gcccgctgca tcaggggtgt ccagaaagaa cggttccgct cctgcgagca gcgccgcacc
       61 ctcgtagatc tgataacacg gattcggcag ggcgacgagc ggctcgcctg ccccatccac
      121 catggcctgc acgaacgaga agagtgcctc gcgtgtgccg ttgaccggca gaatcatggt
      181 ctgcggatcc acggagcgtg agggcagcgc gaagcgtcgc tcgagccagc gcgccgcggc
      241 ggcgcgcagc tccggcacgc cggcggtggt cggatagctg tccagcagag agctgttctc
      301 gcgcagcttg tccacgatga acgccggggg tgcatgccgc ggctccccga tggacatggc
      361 tatgggagtg agcgaccgcg gtggcgtgag cccagccttg agccgcgcga gccgctcgaa
      421 aggataggcc tgaaggcgat ccaggcgcgc attcccgtgc gggctcatgc ggcctggcga
      481 cgatcgagcg cgtgcagcaa ccgatcacgc agatgctgtg ccgccgccgc gcccaggggg
      541 cgctgctcga aatcggtgat gaagaagaca tcctcggcgc gctcgccgac cgtcatgatc
      601 ttggcatcgt gcagctgcag ccgctcttcc atgaggactt tgcccacatc gctcagcagg
      661 ccgggacgat cgctggcggt cagctcgagc acggagcgtc ggttgcgctc atcgatgctg
      721 accgcaacgt gagttggggt attgaacatg cgtgcctggc gcggggcgcg gcgggaaacg
      781 gcaaatgggg agtcttccgg ctgttgcacg gagcgccaca gcgcgtactc gatctcgctg
      841 tgccgatcgt gatcggtgat cggggcgcca tcatcctcca gcacgtgata cagccccaga
      901 ctgaagccat cgccggtcgg cgtgatgcgg gcatcgacga tgttgagtcc gagctgatcg
      961 aggaccgcgg tcgtatgact gaagccatgc cgctcggccc tggcaaagat cagtacggag
     1021 gtcgtgcccc gcacaccgcg caggtcgagt gcgacgagcg gttcatcgga gccgctgtca
     1081 cgctcggcca gcaggcgcgt gtgccaggca acctcatcgg gcgtgtgccg caggaagtag
     1141 gcagccgaaa agcgcctcca tgccccatcg acatcgatgc cggtcacgcc ctgctcatgg
     1201 agcagccgcc gggcggtttc ctgcgtttcc cggacgagct gctcctggtc gatcgggctc
     1261 tccagcccgc gccgcagcgc gcgcttcacc cgatcgaaga actccctgaa gagggaggcc
     1321 ttccaggagt tccacagctt cggattcgtg ccgcgcacat ccgcacaggt gagtacgtac
     1381 aggtagtcga ggtgagcttc atcgccgacc ttgcgcgcga aggcattgat cacctgcgga
     1441 tcggagatgt cctgtttctg agcggtgatc gacagctcca gatgattgcg cacgagccag
     1501 gcgacgagcc gcgcatcgta gggagagagc ccctgctcga ggcagaaggc ctcggcatcg
     1561 accgcgccga gctccgaatg atccccgcca cggcctttcg cgatgtcgtg aaacaacgcg
     1621 gcgagatagg cgatctccgt cctcgggagc tgctgcatga tgcgcgaagc ctcgggcagc
     1681 tcgtggtcgt agcgggagat ggcgaaacgc cgcaaattgc tgaccacgaa aagcgtgtgg
     1741 gcatcgactg tataggcgtg gaagagatcg tactgcatgc gtccgacgat gcgaccgaat
     1801 gcgggaatgt atcgtcccag gacgccgtag gtgttcatcc tgcgcagctc gtgagtgacg
     1861 cccaccggtg cgcgcacgat ctcgaggaac aggcggtgat tgcgtgggtt ctggcggaac
     1921 tcctcatcga tcagccacag gccacgagcg accgcgcgca tggtcgaggc gcgcacgccg
     1981 ttgatgtcgg gactctgctg cagcagtacg aacatctcga ggagcgccga ggggttgcgg
     2041 acgaacatgt cggcgctcac cgcttcgagc gagccgttgc gaacctgaaa gcgtgcattc
     2101 aacggtcgcg gcggctcgct ctcgctgagg atcgcttcgc gaaacacctg gcgcagcagc
     2161 tcgttcagca gactgacatc catcacggtg cggtagtaac gctgcatgaa ctgctcgacc
     2221 gccagggtgt aggaggcatc ctcgtagccg aagctgagcg cgagccggac ctgataatcg
     2281 aacagcagcc ggtcttcgcg gcggccggtg agcgcgtgca gcccgaagcg caccttccac
     2341 aggaaggctt gcgcctgttt caggcggcgc agctcggcca tggacagaaa gccgcgtgtc
     2401 gccaggccat cgagcgtttc cgatccgaag tgacgcttgg cgacccacgc gatggtctga
     2461 atatccctca gtccacccgg tccggtcttg acgttcggct cgaggttgta agcggtgtcg
     2521 ttcgctttca gatggcgctc ggtctgctcg cgaaccttgg cttcgaaaaa ttcgcgtacc
     2581 ggccatacgt gctcgggcga cagcgccgcg agcatcgcct cgaaaagcgc gctgctgccg
     2641 tccacgagcc gcgcctcgag gagcgtcgtc atgacgctga catcggcttt ggcttcgtgc
     2701 acgcactcgg cgatcgtgcg cacactgtgg ccgacctcaa gaccgatatc ccagaggaag
     2761 gtgacgagcc cctccgccgc ctcgcgtccg ccgctctcgg ccggcgccag cagcatgatg
     2821 tcgatgtccg agcagggatg cagctcgccg cggccgtaac cgccaactgc cacgagcgcc
     2881 cagctgtcgg catcctcgag ctggcgcatc cgccacacat gccgcagcac cagatcgatc
     2941 agactggcgc gcgcatgaac gagatcctcc accggctctt cggcgaggaa gcgggccttg
     3001 agctcctcgt ggccctgttt cagaagctcg cgataggctg ccggggagcc ctgggcctga
     3061 gcgagcaggg aaggcaggcg cacgagcagc ggccaggggg cactgcgcgt cggggccggc
     3121 agggtgtcct cgagcaggtc caggtccggc gagggcgtga ccatttcagt cgccgtcagg
     3181 ccgcctgggg cgtgttcgct gcgcccagcg tgagcacctc gtagccgctg tccgtgacga
     3241 gcacggtgtg ctcccattga gccgaaagcg agtgatcctt ggtgatcacg gtccagccat
     3301 ccgggagcag ccggacgtgg cgtttccccg cattcaccat gggctcgacg gtgaatgtca
     3361 ttccgcgttg cagctcgagt cccgtaccgg gctccccgta gtgcagcact tgcgggtcct
     3421 cgtggtacac gcgcccgatc ccgtgcccgc agtactcgcg cacgaccgag tagttgcgct
     3481 cctcgacgaa tgactggatg gcgtgtccga tatcgccgag tcgtgtgcca ggcctcaccg
     3541 tatggatgcc gcgccacatg gcctcgaagc aggtctcggc cagtcgctgc gcgtgcggcg
     3601 ggggcttgcc gacgaagtac atgcggctcg tgtcgccgtg aaagccgtcc cggatcaccg
     3661 tcacatcgat gttgacgatg tctccggcct tgagccgctt gtcgttcgga attccgtggc
     3721 agaccacgtg attcaccgac gtgcagatcg tcttcggaaa tccgcggtag ttgagatttg
     3781 ccggaatgga atgctgcacc ttgacgatgt ggtcgtggca gatccggtca agctcgtcag
     3841 tcgtcacgcc aggcaccacg tgctcgccga tcatgtcgag cacctctgcg gcgaggcgcc
     3901 cggcgatgcg catcttttcc tgctcttcgg gggtcttgat ggtcacgtgc atggtgggcg
     3961 gtgcgaggct cctgggtcga gcgcgtaaga cattgttcag acgggaggtt catggtataa
     4021 agcgccgcct tttttggcaa cgcaacccca cacgcgcatc acgtacccgg cgactgggtg
     4081 tcctcgaggc cgcgcctccc gcaagggggt gctgcggacc acgaggatgg tcaccgggga
     4141 tgcgcggagg cctaacccaa gcaggagttt tttatgcccg gcgtgtccat gcgacagatg
     4201 ctggaggcgg gtgtccactt cggacatcag acccgcttct ggaacccgaa gatggctccc
     4261 ttcatcttcg gtgagcgaaa caagatccac atcatcaacc tcgagaagac gcagcccctg
     4321 tattcggagg cggcgaactt cgtgaagggc gtcatcgccg acggcggcac ggtgctgttc
     4381 gtgggcacca agcgctcggc gcgcgaggcg gttcaaaaag aggccgtgcg cgccaatcag
     4441 ccgtacgtca atcagcgctg gctcgggggc atgctcacga acttcaagac catccgccag
     4501 tcgatcaagc ggctgggcga gatcagcgag ctcgcgagct cgggcgcgct ggagaagcgc
     4561 ggcaagaagg aagccaccca gctgcggcgc gagatggaca agctcgagag gagcctgggc
     4621 ggaatcaaga acatggaatc cctcccggat gccatgttcg tggtggacgt cggccacgag
     4681 aagatcgcga ttcacgaggc tcgcaagctc gggattccgg tggtggccat cgtggacacg
     4741 aactgttccc cggatggaat cgactacgtg atccccggca acgacgatgc gatgcgggcg
     4801 attcagctct atgcgacggg catggccgat gcggtgctcg agggcaagga gtccgtaccg
     4861 gcggtcgcgg tgggcgaaga tgagttcgtc gagctcgatg aagccggcca tccccgtaag
     4921 aaaacggctc gcggccgcca gcggccgcag gccaccgtgc gcaagaaagc gccggcccgc
     4981 cgcaagctgc ccccggtcaa ggcgccgccc gggcccaccg aggaggtcgc caccgaagtc
     5041 gaagtggcag tcgaagtcga tgatgaggat ctggatgctg acgacgtgac ggatgtcgtc
     5101 acggcagccg gcgtgacacc cgtggcgcca gggggtaccg agcgtcgccc ctcagcgagc
     5161 gctcggcgcg gcaagggggg ccggggccct gcgccaggtt cctcgcggga gtaagagcac
     5221 cttatgaata tcacagcaga tgcggtcaaa cagcttcgcg agcgcaccgg tgccggcatg
     5281 atggagtgca agcgcgcgct ggtggaaacc cagggcgatc tggatgccgc cgccgagctg
     5341 atgcgcaagc agggacacgc caaggccgac aagaaagcgg cccgcgtggc ggccgagggc
     5401 gtggtgatca tcgagaaatc tgccgatggc cgcaccgcgg ccatggccga ggtcaactgc
     5461 gagaccgact tcgtcgcccg cgagcaggat tttcgcgcct tcgcccaggt ggttgccgcc
     5521 cgggcactgg ctgacagatc gccctcactc gaggcgttgc tggcggcacg cgcgggcaat
     5581 gcgacggaaa ccatcgagga acgcaggcgc gcgctcattg ccaagatcgg cgagaacatc
     5641 agcgtgcgcc gcttcgtcgt tctgagctcc tccgatcatc tcggcgcgta cgtgcacggg
     5701 actcggattg gcgcgctcgt cgtggtgacc ggcggggatg ccgcacttgc gcatgatctc
     5761 gccatgcatg ttgctgcgag caatcctcgg cacctgagtg cggaggatgt gccggccgag
     5821 gtcgtggcga aggagcgcga gatcctcacg gagcaggcgc agggtgaggg caagccgccg
     5881 cagatcgtag ccaagatggt cgaaggccga ttgcgcaagg cgctcagcga gatcaccctc
     5941 accggacagc cgttcgtgaa ggaccccgat gtgagcatcg agaagttgct caagagcgcc
     6001 ggggcccagg tgactgcgta cgcgcgcttc gaggtcggtg cggggatcga gaagaagcag
     6061 gatgacttcg tcgcggaagt gatggcgcag gtgaagagcg cgacgccgcc ggcgccgggc
     6121 acgaccccgc cggtcaagca ctgaccgtgg ccggcggttc ggagctgcag gcgctgatgc
     6181 gctggcctcg atccgcagcg cgctgcttcc tgcgggaact cccggcgcgg ctggcagcag
     6241 gcaaaacccc gccacgtgcg gggttttttt ggaccacacg aggagcagcg caatgagcga
     6301 tgcagcgaag ccgagatact cacggatcct cgtgaagctt tccggggagg cactgctcgg
     6361 gtcggcggac tatggcgtgg accccgccgt gctcaagcgg atcgccggcg agctgcagga
     6421 catcatcgag atgggcgtgc aggttgcggt cgtgatcggc gggggcaaca tctttcgtgg
     6481 ggcggggctt gcgcgcgcgg gaatggatcg ggtcgcggcc gatcacatgg ggatgctagc
     6541 cacggtgatg aattccctcg ccatgcagga tgcgctcgaa gcgctcggga tgtacgcccg
     6601 cgtgatgtcg gcgatccgta tcaacgaggt gtgtgaggat tacatccgcc ggcgggcggt
     6661 acgccacctc gagaaaggcc gggtcgtcat ctttgccgcg ggcaccggca accccttctt
     6721 caccaccgac accgccgcat cgctgcgcgc gatcgagatc aacgccgata tcctgctgaa
     6781 ggctaccaag gtgaacgggg tgtattccga cgatccggtg cggaacccgg cggccatccg
     6841 ctatgcgaca ctgacattcg accgggtgct cgctgacaag ctcaacgtca tggacgccac
     6901 cgccatcgtg atgtgccgtg acaaccgttt gccgttgcgg gtgttcaacc tgaacaattc
     6961 cggggacctg acgcggatcg tcaggggtga ggatgtagga acggcagtga cgttcgagta
     7021 gtctcgctcg ggcgtgagcc cgtgaggaat atcagtatgc tcgaggatct gaagaaggat
     7081 gcgcgcgagc gcatggcgaa gtgtgtccag accttccagg gcgatctgaa gaagctgcgc
     7141 acggggcgcg cgcaccccag cctgatcgag cacctgagag tggactacta cggctccgag
     7201 gtgccgctgc agcaggtggc caacatcgcc gtggaggatg ggcgcacgct cgtggtctct
     7261 ccctgggaga agtcgatggt gcagcccatc gagaaggcga tccacaagtc cgacctgggc
     7321 ctcactccga tgagcgccgg cacggtgatc cgtattccca tgccgccgct caccgaagag
     7381 cggcgccggg atatcaccaa ggttctgcgc cacgatgccg agaatgcgcg cgtcgctgtg
     7441 cgcaatgtgc gccgcgatgt catggccgat atcaaggagg cgctgaagga gaagctcgtc
     7501 tcgcaggatg atgagcggcg cgccgagacc gaagtgcaga agctcactga caagcacgtc
     7561 gccgacatcg accagctgct ggcggcaaaa gaaaaagagg tcatgcaggt ttgacgaggc
     7621 tgccgcggca cgtcgcgatc acgatggacg gcaacgggcg gtgggccgcg gcccgcgggg
     7681 tgccgcgcgc ggcaggtcac cgcgccgggc tcaagccgac gcgcatggtc atccaggagt
     7741 gcgcgcgccg cggcgtcgaa gccctgacgc tttttgcatt ctccagcgag aactggaagc
     7801 ggccggcgga cgaggtctcc agtctcatgg cgttgttcgt cgactcgctc gaccgcgaga
     7861 tcgatgagct gcatgcccga cgggtgcggg tgcgcttcat cggcaatcgc ggtcggctgg
     7921 ccatgcgatt gcagacacgc attgccgagg ccgagcaacg cacggccgcc aatcccgggc
     7981 tgaagctgca gatcgcggta agctatggcg gtcgatggga tattatccag gcagctcaaa
     8041 aacttgcagc gcaatgctca agtggattta tacgcgccga ggacatcgat gagccgcgct
     8101 tcggcgcgac gctcgagctc gccgacgtgc ccgacgccga tctcatgatc cgcaccgggg
     8161 gtgagcagcg catca
//
LOCUS       DASRHN010000261         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1075596, whole genome shotgun sequence.
ACCESSION   DASRHN010000261 DASRHN010000000
VERSION     DASRHN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075596"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFB37_10355"
     CDS             <1..358
                     /locus_tag="VFB37_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22890.1"
                     /translation="LGSSSSHTYGFPGATPSVSASSASSNGIVWSIDGSRYCTLESPG
                     CGPAVLYAHDASNLATELWNSSKVATDAAGNGVKFTVPTVANGSVFVGTRGNNTGGAD
                     SSSSTPGELDIYGLIP"
     gene            519..1847
                     /locus_tag="VFB37_10360"
     CDS             519..1847
                     /locus_tag="VFB37_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZO22891.1"
                     /translation="MLERKRLKRWSVPGWVLLCAPGLALCADSASQEVDAAYPKAFAL
                     YTELHQHPELSGFEVQTAATLAGQLRSLGYSVTEHVGGTGVVAVLKNGAGPIIMLRTE
                     LDALPVEEKTGLAYASKVRTKDAAGREVFVGHMCGHDLHMSALVATAGIMARSKDSWH
                     GTLMLIGQPAEETISGASKMIRDGLFTRFPKPSAVLALHVGNALPAGKVGVVPGIYST
                     NADSVRLTIYGKGGHGSAPHTAVDPIVTAARTILALQTIVSREVKPGEMAVVTVGYIT
                     AGTKNNIIPDQAELGLTVRTFKPEIRKQVLAAITRITNAEAQAAGAPRPPSIDVYEAT
                     EAVYNDPALTQRMRSVLESAIGKDNVLVSEPMTASEDFSAFVATGIPGFYLTLGGADP
                     EKYAEAKATGTHLPSNHSSLFAPDVDPALHVAISSEVAMLRSLLKSGSGA"
     gene            complement(1920..3686)
                     /locus_tag="VFB37_10365"
     CDS             complement(1920..3686)
                     /locus_tag="VFB37_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22892.1"
                     /translation="MSSVPIQPASRQLPHSASDQPAQPASSPAVDSASFRDTNGLLPL
                     IIGVTGHRDLDRAHYPVYRARIVEFLAHLRRQYPSTPLRILSSLAEGADRLAAEVALE
                     QGSELLVPLPLEPAEYERDFPESTAEFRALLQRVPAEHVFVLEHTAGHAPPHWSAAGH
                     RDLSYREAGAYMAAHSHILLALWDGQDNDSIAGTSAIVRLKLEGQSWSRSLDPRALDS
                     DDTGPVFHVHSPRVGSNSSADLPARWLFPRDADAKQFHVVCRYLERFNRDALEARDEL
                     ASTEAAASLLPELESRPMGDRAVARIFGHADRLARHHQRLTHAVLRLILGLAAVLALT
                     FEVYAEILPRREVPAVYLLVFCAVAGLYLWQKRQDAQGRYLDYRALAEGLRVQFYWRV
                     AGLRDSASANYLRKQLDELRWIREALRGANTVPPSRQPRLDLVMRYWVQGQAGYYRTR
                     ARLQATRISRVEHLSSVCFATGLLATAVLVIFWNRMSPLGEWRHWLVVVMGFAPIAAA
                     LWEAYGERFGLRTQAHQYARFATIFGRAEQAIERLEATPAEKRRHESERALIRELGRE
                     SLMETGDWVLLWRDRPIALPKG"
     gene            complement(3683..4363)
                     /locus_tag="VFB37_10370"
     CDS             complement(3683..4363)
                     /locus_tag="VFB37_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZO22893.1"
                     /translation="MGRSVFVSYSEPDRTCALELVSHLEAQAISVWVAPRDVAPSADW
                     AAEIIDAISSSRLMVLVFSAHSNASPQVRREVERAIHKQIPVLPFRIENVLPSKSLEY
                     FLSTQHWLDAYTLPREQHYARLCTHVSCLLKLTADTPELPAQSAAGAGAKRPKLSVTV
                     SEQQFLEKTLAFHVGPVAKLLVKRALAEAADWDQLTQRLASELDSDSARRRFVASCQS
                     TTTLRLIV"
     gene            complement(4366..>4946)
                     /locus_tag="VFB37_10375"
     CDS             complement(4366..>4946)
                     /locus_tag="VFB37_10375"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HZO22894.1"
                     /translation="YKEATLNPTNPRDRATDWEADLIQKFRNDAHTHEIVGERDTPTG
                     RSLYMARPIQVESECLGCHSLPSAAPASLLARYGRDNGFGWQAHEIVGAQIVSVPLAS
                     AVASADRMFWRIMSSILIVLIFALLLVNSMLYYLVVRPIRRIAHVADELSLGNPAPEA
                     FPARGSAEVTALGRAFERMRTSLDKALKLLES"
BASE COUNT          880 a         1590 c         1649 g          827 t
ORIGIN      
        1 cctgggctcc tcctcgagtc atacctatgg attcccaggt gcgaccccgt cggtatccgc
       61 ctcttcggcg agcagcaacg gcatcgtctg gagcatcgac gggagccgct actgcacgct
      121 cgagtcccca ggctgcggtc ctgccgtgct gtacgctcac gatgcgagca atctcgccac
      181 cgagctttgg aacagctcga aagtggctac cgatgcagcc ggcaacgggg tcaagttcac
      241 ggtccccacg gtcgcgaatg gctcggtgtt cgtgggaacc cgcgggaaca acacgggtgg
      301 ggccgacagc tcttcgagca cgcccggcga gctcgatatt tacggcctga taccctgatc
      361 ggtctgtgaa ctggcgggcg gacggcgccg tccgcccgcc gtctcggagg cttcgcgggc
      421 gacctccggg ctgccgcatc ggatcccgga ccctgagcaa gcggtttccg gtacatttgc
      481 gcattgttca atgcgcagac accaccgggg tcatcgatat gctcgagcgg aaacgattga
      541 agagatggag cgtgcccggt tgggttctgc tgtgtgcccc cgggctcgcc ctctgcgccg
      601 attcggccag ccaggaggtc gatgcggcct acccgaaggc gttcgcgctc tacaccgagc
      661 tgcaccagca tcccgagctt tccggatttg aagtccagac cgccgccacc ctcgctggcc
      721 agctccgcag cctcggctac tcggtcaccg agcacgtcgg cgggaccggt gtggttgccg
      781 tgctcaagaa cggcgccgga ccgatcatca tgctgcgcac ggagctcgat gcgctgcccg
      841 tggaggagaa gaccggcctt gcctatgcca gcaaggtgcg caccaaagat gcagccggga
      901 gggaagtctt tgtcggacac atgtgcggac acgatcttca catgagcgca ctggtggcca
      961 ccgcgggcat catggcgcgc agcaaggaca gctggcatgg caccctgatg ctcatcggcc
     1021 aacctgcgga ggagacgatc agcggggcct cgaaaatgat ccgcgacggc ctcttcacgc
     1081 gctttcccaa accctctgcc gtactcgccc tgcatgtcgg taatgcgttg ccagctggta
     1141 aggtgggcgt cgtgccgggc atttattcca ccaacgccga ttcagtgcgg ctcaccatct
     1201 atggaaaggg cggacatggg tcggcaccgc atacggctgt cgatccgatc gtgacagccg
     1261 cgcgcacgat ccttgcgctg cagaccatcg tatcgcgtga agtcaaaccg ggtgagatgg
     1321 cagtggttac cgtggggtac atcaccgccg gcaccaagaa caacatcatt cccgatcagg
     1381 ccgagctcgg attgacggtg cgaacattca aacccgagat ccgcaagcag gtactggccg
     1441 cgatcacgcg gatcacgaac gcggaggcgc aggcggccgg ggcgccgcgc cccccttcga
     1501 tcgatgtcta cgaagccact gaggcggtct acaacgatcc cgctctcaca cagcgcatgc
     1561 gctcggtgct cgagagcgcg attggcaagg acaacgtgct cgtgagcgag ccgatgacgg
     1621 cttccgagga cttctccgcc ttcgtggcga cgggaattcc cggcttttat ctcactctcg
     1681 gcggggctga tcccgagaag tacgccgagg cgaaggccac ggggacgcac ctcccgtcaa
     1741 accattcctc tctcttcgcc cccgacgtcg atccggcgct gcatgtggct atcagcagcg
     1801 aagtggccat gctgcgcagc ctgctcaaat ccgggagcgg cgcctgaggg caggtcgccc
     1861 gggctctgtg cgccgggcac ttccgcgtgg cggctccgcc gcgcggggtc agggcgagat
     1921 cagcccttcg gcagcgcgat cgggcggtcg cgccacagga gcacccagtc ccccgtttcc
     1981 atgagggatt cgcgtcccag ctcgcggatg agggcgcgct cgctctcgtg ccggcgtttc
     2041 tcggcgggcg tggcttccag tctttcgatc gcctgctcgg cgcggccgaa gatggtggcg
     2101 aagcgcgcat actggtgcgc ctgcgttcgc aggccgaatc tttcgccgta ggcctcccac
     2161 agagccgcgg cgatcggcgc aaatcccatc acgaccacga gccagtgacg ccactcgccg
     2221 aggggcgaca tccgattcca gaagatgacc agcaccgcgg ttgcgagcag gcccgtggcg
     2281 aaacagaccg aggacagatg ctcaactcgg ctgatgcgcg tggcctgcag acgcgcccgg
     2341 gtgcggtaat acccagcctg accctgcacc cagtaacgca tcaccagatc gagccggggt
     2401 tgcctcgagg gtgggacggt gttcgcaccc cgcagcgcct cgcggatcca gcgcagctca
     2461 tcgagctgct tgcgcagata gttggccgag gcgctgtcgc gcagaccggc gacgcgccag
     2521 tagaattgca ctcgcagccc ttcggcgagt gctcgataat ccaggtaacg accctgcgca
     2581 tcctggcgtt tctgccagag gtagaggcct gcgactgcgc agaagacgag cagatacacc
     2641 gccggcactt cccggcgcgg cagaatctcc gcgtacactt cgaaagtcag tgccagcacg
     2701 gcggcaagtc ccaggatgag gcgcagcacc gcgtgcgtga gacgctgatg gtgacgcgca
     2761 agccggtcgg cgtgaccgaa gatgcgcgcc acggcccgat cccccatggg acgactctcg
     2821 agctcgggaa gcaatgatgc ggccgcttcg gtgctcgcga gctcatcgcg tgcttcaagc
     2881 gcgtcgcggt tgaatcgctc caggtatcga cagacgacgt ggaactgctt tgcatccgca
     2941 tcgcgcggga acagccatcg ggccggcaga tcggcgctcg agttactgcc cacacgtggt
     3001 gagtgcacgt gaaacaccgg ccccgtgtca tcggagtcga gtgcccgcgg atcgagcgag
     3061 cgggaccatg attgtccttc cagcttcagg cgcacgatgg cggaggtccc ggcgatcgag
     3121 tcgttgtcct gcccgtccca gagcgctagg aggatgtggc tgtgcgccgc catgtaggct
     3181 ccggcctcgc ggtaactcag atcgcgatgg ccggcggcgg accagtgagg gggggcgtgg
     3241 ccggcggtat gctcgagaac gaagacgtgc tctgcaggga ctctctgcag cagcgcccgg
     3301 aattctgcgg tcgattcggg aaagtcgcgc tcgtactcgg ccggctccag aggcagcggc
     3361 acgagcagct cgctgccctg ctcgagcgcc acttcggcgg cgagccggtc ggcgccctct
     3421 gccagtgagg acaggatgcg cagcggcgtg gaggggtact gccgccgcag gtgcgccaga
     3481 aactccacga tccgggcccg ataaaccggg tagtgggctc ggtcgagatc gcggtggccg
     3541 gttacaccga tgatgagcgg cagcagcccg ttggtatccc ggaagctggc cgagtccacc
     3601 gcagggctgg aggctggctg cgcgggctga tccgaagccg agtgcgggag ctgacgcgag
     3661 gccggttgga tggggacgct gctcaaacaa tcagccgcag tgtggtggtc gattggcagg
     3721 acgccacgaa acggcggcgc gcggagtcgg aatcgagctc cgaggccagc cgctgcgtga
     3781 gctggtccca gtcggcagcc tcggcgagtg cgcgcttcac cagcagcttc gccaccggac
     3841 cgacgtgaaa ggcgagggtc ttctccagga actgctgctc gctgaccgtg acggaaagtt
     3901 tgggtcgctt cgcgcccgcg ccggcggcgg actgtgcagg cagctcgggg gtgtcggcag
     3961 tcagcttcag caggcaactc acgtgagtgc acagtcgggc gtagtgctgc tcgcgtggca
     4021 gcgtgtacgc atcgagccaa tgctgcgtgc tgagaaaata ttcgaggctt ttcgatggca
     4081 gcacattctc gatacgaaag ggcagcacgg gaatctgctt gtgaatggcg cgctcgactt
     4141 cgcgacgcac ctgcggcgag gcgttactgt gcgccgagaa gaccagcacc atgagacgtg
     4201 acgaggagat cgcgtcgatg atctccgcgg cccagtccgc cgatggagcc acgtcacgag
     4261 gggcaaccca gacgctgatg gcctgcgcct cgagatgcga gaccagctcg agcgcgcatg
     4321 ttcggtcggg ttcggaatag ctcacgaaaa cgctgcgtcc catgctcatg actccagcag
     4381 cttcaacgcc ttgtcgagac tggtgcgcat ccgctcgaat gcgcgtccca gggccgtgac
     4441 ctccgcactg ccccgagcgg ggaatgcctc cggtgccgga ttgccaaggc tcagctcgtc
     4501 agccacgtgc gcaatgcgcc ggatgggccg tacgacgagg tagtagagca tgctgttcac
     4561 cagcaacagg gcgaagatca gcacgatgag gatggagctc atgattcgcc agaacattct
     4621 gtcggcgctc gccacggcgc tcgccagcgg gaccgagacg atctgggcac ccacgatctc
     4681 atgcgcctgc cacccaaagc cattatcacg gccgtaacgc gccagcagcg aggcgggggc
     4741 cgccgagggc aggctgtgac agccgaggca ctcggattcg acctggatgg gccgcgccat
     4801 gtacagcgag cgccccgtgg gggtatcccg ttcaccgacg atctcatgcg tgtgggcgtc
     4861 atttctgaat ttctggatca ggtcggcctc ccagtccgtg gcccggtcgc gcggattcgt
     4921 cggattcagc gtcgcctcct tgtagg
//
LOCUS       DASRHN010000262         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1411250, whole genome shotgun sequence.
ACCESSION   DASRHN010000262 DASRHN010000000
VERSION     DASRHN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411250"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..227
                     /gene="ffs"
                     /locus_tag="VFB37_10380"
     ncRNA           130..227
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFB37_10380"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            383..2065
                     /gene="dnaX"
                     /locus_tag="VFB37_10385"
     CDS             383..2065
                     /gene="dnaX"
                     /locus_tag="VFB37_10385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZO22895.1"
                     /translation="MSYTALARTWRPKTFADLVGQEHVRQALMNALRSGRIHHAFLFT
                     GTRGVGKTTIARILAKCLNCEKGVTAEPCGVCASCREIDAGRFVDLIEVDAASRTKVD
                     DTRELLDNVQYAPTRGRYKVYLVDEVHMLSTHSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFNLKRLPIGQIAERMREILEKEGTAFDAAALRLLAQAADGSLRDAL
                     SLLDQLLAFGGGSAREAEARAMLGTVARDHVVRLAEVLGTMNVPELLSCARSLEEWAP
                     DYAQVLDELASLLVRIAMKQAVPDFEGDELYAPEVLERLASVFAPEDVQLFYQTAVIG
                     RRDLALAPDPRTGFQMTLLRMAAFRPAAAAGASHPLPDRGRSAGSSQQSPAEAAAVSS
                     ADPLARAAAAGGLAGGRSETTAPPSAAGAASTASRETSNSPEVPGSWAAILSELDLQG
                     AARQLASRCALLGRQGGLVRLALDGRNKFLRTPSQEEKLAQALSRYFGESVRLEFVAA
                     PAGAETPAQADERASMQDLDGARRAFESDPGVQLLREQFGATVLADTIRPLK"
     gene            2093..2416
                     /locus_tag="VFB37_10390"
     CDS             2093..2416
                     /locus_tag="VFB37_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006792396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZO22896.1"
                     /translation="MRGNFGNMMKQAQAMQANMQKVQAEIATIEVSGESGGGMVKVIM
                     NGRHEVKRVQIEPSVAGEDREMLEDLLAAAINDAVHKVEARVQEKMAAVTAGLQLPPG
                     MKLPF"
     gene            2454..3053
                     /gene="recR"
                     /locus_tag="VFB37_10395"
     CDS             2454..3053
                     /gene="recR"
                     /locus_tag="VFB37_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZO22897.1"
                     /translation="MKHSPRLTQLIDALRALPGVGPKTAQRMAFHLLQEGRPGARALA
                     DALEGALTAVTRCARCRMLTEGSVCSICSATHRDASLLCVVESPADVVAIEQSGSYRG
                     RYFVLMGHLSPLDGVGPEQLGARELEAILGEGGVRELILATNPTVEGEATAHFLGELA
                     TRRGIRASRIAHGVPIGGELEYVDGGTLAHALAGRQSVS"
     gene            complement(3082..>3804)
                     /gene="rsgA"
                     /locus_tag="VFB37_10400"
     CDS             complement(3082..>3804)
                     /gene="rsgA"
                     /locus_tag="VFB37_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZO22898.1"
                     /translation="GAEPVVANLSQLLVVLAPMPVPDLFVLDRYLAAATSGAIPAKLL
                     VNKSESGIAGDLLAELEVYRSAGYDWLQCSVRSGEGMASLAATCAGAITALVGQSGVG
                     KSSLIRQLVPEARIEIGALVRDEEGRHTTTAARLFDMPTGGSLIDSPGVRDFAPAIDR
                     LDRAHLGFIEVARLAQRCRFKDCRHMREPGCAVSAAAAQGTLHPRRYESYRRLRRLYE
                     DLTQARGPQSRGPSGRRPVRRS"
BASE COUNT          625 a         1223 c         1326 g          630 t
ORIGIN      
        1 ggggaaagtc aatctacttt gacggcacgc cagcgatgcc ccgtgtcctc gagaggcaga
       61 tgacttgcgc accttcgcat tgagccccat gttctgagag ctttgcggct cgcggtttat
      121 ggtggctcgc atcggcttct ccgcgacgac cagtcgtgaa tcccgtcagg gccggaaggc
      181 agcagcggca gcggcgatgt cgggtgccgg agctacggct ggtgcgggcc acctcctgcg
      241 aaagctcgaa cgggcgcgtg cgggagccga ccgtgggtga caggccgcga tggcaaggcc
      301 cgcggtgaga gccgcggtac gagaggctcg cgaggcgagg gcggatgaga ggcgcagcgg
      361 agagctccgc acaagtgaag tcatgagtta cacggccctg gcgcgcacct ggcgcccgaa
      421 aactttcgcc gacctcgtcg ggcaggaaca tgtccgccag gccctgatga atgcgctgcg
      481 gagcgggcgc atccaccatg cgttcctgtt caccggaaca cgtggggtgg gcaagaccac
      541 gattgcgcgg atcctcgcca aatgtctcaa ctgcgagaag ggcgtgacgg ctgagccgtg
      601 cggtgtctgc gcgagctgtc gtgagatcga tgcgggccga ttcgtggacc tgatcgaagt
      661 ggatgccgcc tcgcggacca aggtcgacga cactcgcgag ctgctcgaca acgtgcagta
      721 cgcgccgacg cgaggccggt acaaggtgta tctcgtggac gaagtgcaca tgctctccac
      781 gcactcattc aatgcgctgt tgaaaacgct cgaggagccc ccgccgcacg tcaagttttt
      841 gctggcgacg accgatccgc agaagctgcc cgtgaccgtt ctgtcgcgct gcctgcagtt
      901 caatctgaag cgcttgccga tcggacagat cgcagagcgc atgcgtgaga ttctggagaa
      961 ggaaggcact gccttcgatg ccgcggcatt gcgtctgctc gcgcaggcgg cggacggcag
     1021 cctgcgcgat gcgctgtcgc tgctcgatca gctgctcgca ttcgggggcg ggagcgcccg
     1081 ggaagccgaa gcccgcgcca tgctgggaac ggtcgcgcgg gatcacgtgg tgcgcctcgc
     1141 agaagtgctg ggcacgatga acgtgcccga gctgctgagc tgtgcgcggt ctttggagga
     1201 gtgggcgccc gactatgcac aagtgctcga tgagctcgcc tcgctcctgg tgcgcatcgc
     1261 gatgaagcag gcggtgccgg acttcgaggg cgacgagctt tatgcgccgg aggtgctcga
     1321 gcggctcgcc tcggtgtttg cgccggaaga cgtccagctg ttttatcaga ctgcggtcat
     1381 cggacggcgt gacctggccc tggcgccgga tccgcgcacc ggcttccaga tgaccttgct
     1441 caggatggct gcgtttcggc cggccgccgc agccggagca tcccacccgc tcccggatcg
     1501 cggccgatcg gcaggatcct cgcaacaatc accggcagag gccgcggcgg tcagctctgc
     1561 cgacccgttg gcgcgcgcag ctgctgccgg aggattggct ggcgggcgtt cggagaccac
     1621 cgcgcctcca tctgccgcgg gcgcggcctc aaccgcctca cgcgagacca gcaactcgcc
     1681 agaggtcccc gggtcgtggg cggcgatcct gagcgagctc gatctgcagg gcgcagcccg
     1741 tcagctcgcc agccgctgcg cgctgctcgg ccgtcagggc gggttggtgc gtcttgcgct
     1801 ggatggccgc aacaagttcc tgcgcacgcc ctcgcaggag gaaaagcttg ctcaggctct
     1861 gtcccgatat ttcggcgaga gcgtgcggct ggagttcgtt gctgcgcctg ccggtgccga
     1921 gaccccggcg caagcggacg agcgcgcctc catgcaggac ctggacggcg cgcgccgcgc
     1981 tttcgagtcc gatccgggcg tgcaactgct gcgcgagcag ttcggcgcca ccgtgttggc
     2041 cgatacgatc cgtcctctca agtaaccgtt tcgatgctca ggagtcagtc ccatgcgcgg
     2101 taatttcggc aacatgatga agcaggccca ggccatgcag gcaaacatgc agaaggtgca
     2161 ggccgagatc gcgaccatcg aagtgagcgg ggagtccggt ggcggcatgg tgaaggtcat
     2221 catgaacggc cggcacgagg tcaagcgtgt gcagatcgag ccctccgtcg caggcgagga
     2281 tcgggagatg ctcgaggatc tgctggcagc ggcgatcaac gatgccgtgc acaaggtgga
     2341 ggcgcgcgtg caggaaaaga tggcggccgt gacggccgga ctgcagctgc cgcccgggat
     2401 gaagttgccc ttctgatgcc cgcgcacagg tttcgggccg cgcgctgcct gacatgaaac
     2461 actccccccg cctgacccag ctcatcgatg cgctgcgagc ccttccaggt gtcgggccga
     2521 aaaccgctca acgcatggcc tttcacctcc ttcaggaggg acgccccggg gcgcgtgcgc
     2581 tcgcggatgc gctcgagggt gcacttaccg ccgtgacccg ctgtgcccga tgccggatgc
     2641 tcacggaggg atctgtctgc tcgatctgct cggccacaca tcgcgacgcc tcgctcctgt
     2701 gtgtcgtgga atcaccggcg gacgtggtgg ccatcgagca gtcgggaagc taccgcggcc
     2761 gctacttcgt cctgatgggg catctgtccc cgctggatgg cgtaggtccc gagcagctcg
     2821 gcgcccggga gctcgaagcc atcctgggcg aagggggcgt gcgtgagctg attctggcga
     2881 ccaaccccac ggtggagggc gaggcgacag cccactttct cggcgagctc gcgacgcgcc
     2941 ggggaattcg ggcgagccgt attgcgcacg gtgtgcccat cggtggggag ctcgagtacg
     3001 tggacggcgg gacgctcgcg cacgcgctgg ccggacgtca atcggtcagt tgagccggat
     3061 cgcttccagc tcgggacccg ctcagctacg gcgcacgggt cttcggcccg acgggccgcg
     3121 cgactgcggt ccgcgtgcct gcgtcagatc ctcgtacaac ctgcgcaatc gccgatagct
     3181 ctcgtaacgc cgcggatgca atgtgccctg cgcagcggct gcgctgaccg cgcagccggg
     3241 ctcgcgcatg tgacggcaat ccttgaagcg gcacctctgc gcgaggcgcg cgacttcgat
     3301 gaatcccaga tgcgcgcggt cgagtcgatc gatggcgggc gcgaagtcgc gcacgccggg
     3361 tgaatcgatc agactgccgc ccgtgggcat gtcgaacagc cgcgcggcgg tcgtggtgtg
     3421 ccggccttcc tcgtcgcgca cgagtgctcc gatctcgatg cgagcctccg gcaccagctg
     3481 ccggatgaga gacgacttgc ccactccgga ctggcctacg agagccgtga tggctccggc
     3541 acaggtggcc gccaggctcg ccatgccttc gccactgcgc accgagcatt gcagccagtc
     3601 atagcctgcg ctcctgtaga cctcgagctc cgccagcaag tcgccggcaa ttcccgactc
     3661 gctcttgttg acgaggagtt ttgccgggat ggcgccggaa gtggccgctg cgagatagcg
     3721 atcgagcacg aacagatccg gcacgggcat cggtgcgagc acgaccagca attgcgacag
     3781 gttggcgacg accggctccg cgcc
//
LOCUS       DASRHN010000263         5961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1246191, whole genome shotgun sequence.
ACCESSION   DASRHN010000263 DASRHN010000000
VERSION     DASRHN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246191"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1765
                     /locus_tag="VFB37_10405"
     CDS             278..1765
                     /locus_tag="VFB37_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZO22899.1"
                     /translation="MAWNLHSEGARRKPTPLNVATAGEKMRAAPTTCLLGSSLALLGP
                     AIAWGADAQPLPDKGDTAWMLTCTALVLLMSVPALALFYGGLVRTKNMLSVLMQVFVG
                     FSLITVLWCVYGYSLAFTAGNAFLGGFSRVFLAGTFDPATQRFSMAATFSRHTPLYEL
                     VYVAFQSTFAAITCCLILGSIVERIRFPAVLVFLVIWFTFSYCPIAHMVWYSPGPDAY
                     TPDANADAVSATGGLLWQWGALDFAGGTVVHINAGVAGLVGAVLLGRRTGFGREPMPP
                     HSLTMTMIGASLLWFGWFGFNAGSALEANGSAALAFLNTYLATACAVLSWMFCEWTLK
                     GKPSMLGAASGAVAGLVAITPAAGNVGIPGAFAIGIGAGLVCLWGVTGLKRLIRADDA
                     LDVFGIHALGGIFGALMTGIFNAPALGGPGSVQDWAKGTVGYPGIWTQLRIQMEAVGL
                     VVLWTAVVAGLGFTVVKALMGLRVTEEEEREGLDITSHGERAYDM"
     gene            1830..2315
                     /locus_tag="VFB37_10410"
     CDS             1830..2315
                     /locus_tag="VFB37_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZO22900.1"
                     /translation="MLRSIAIGTLILAGMAATAFADVYRWVDEHGVPHYTDQWVPGAQ
                     VIKSSKPHPATADSDSGDRNSSLQAGAERASELITQQNATQAVKADEAKIRAQQCKEA
                     KDRYEKSLIARRFYKQAKPGEKDDDKTAERAYLSDQEIEAFRVQARTEMEQVCGKQPA
                     P"
     gene            2394..3293
                     /locus_tag="VFB37_10415"
     CDS             2394..3293
                     /locus_tag="VFB37_10415"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO22901.1"
                     /translation="MRVGFAGLGAMGAHMARNLHRAGLLGAVWNRSAEKARSLAAELG
                     CEAPQTLAALASSVDAVVICVSADNDVLEVVRGLAPELRTGALVIDCSTVAAETARSA
                     AALLHPSGVEFLDCPVSGGVEGAQNATLVIMVGGTPEAFEQAQPVLKAMGKTVTLFGP
                     TGSGQAAKATNQIMCAGIIEAVAEAMAFAHAQGLPLERLIDTLGKGAGSSWYFVHRAP
                     NMMRGSYPAGFRVRLHAKDLGICRDMAARFGVALPVVERMLGEYAELVSRGYGDEDIS
                     ATFRLKEELFQRPAKPSATPRDA"
     gene            3290..4372
                     /locus_tag="VFB37_10420"
     CDS             3290..4372
                     /locus_tag="VFB37_10420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZO22902.1"
                     /translation="MKTEPAAGSDKPFYLPDFCTSRAALAIVLIVELTALMLTLARDS
                     ALIGFWTDLARTSLFLLWIGLAGAGLLCWLGEPLKRLPVAQGSAAVLALITGLITVVS
                     LVTFVLGRSTFGGYASIVALFPSEPGTFLWRNVIIGFVVTALALRYFYVTHEWRRNIE
                     MQATARVHALQARIRPHFLFNSMNTIASLTRTHPEVAEQAVQDLADLFRANLSDKRDT
                     ITLEEECEVARTYQRIEQLRLGSRLRIDWKIDPLPRNALVPGLMLQPLLENAIYHGIE
                     PQPEGGTVTVTGELAGGLITIVVRNPVGAQQNEREGNRLALANIRERLGLMYGERGLV
                     KSGRFGPEYIVTLRFPHIESRVKLAT"
     gene            4369..5100
                     /locus_tag="VFB37_10425"
     CDS             4369..5100
                     /locus_tag="VFB37_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZO22903.1"
                     /translation="MKLKVLIVDDEAPARERLASLLAELEGVEIAGEAVNGVQALQRT
                     TELTPDVLLLDVRMPGMDGLEAARHLNALDEPPAVIFTTAYDEYAINAFEAQAVGYLL
                     KPIRKEKLAAALAQAGRLTRPQLQKLSAGTPERRTHIAAKHREGLRLIPLEDVQYFLA
                     EQKYTTVKHLKGEDLIEDSLRALEEEFGSSFARVHRNALINVRYLERIDRNAEGQYCV
                     RLQGCEAPLQVSRRMASELRERFRM"
     gene            complement(5118..>5961)
                     /locus_tag="VFB37_10430"
     CDS             complement(5118..>5961)
                     /locus_tag="VFB37_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO22904.1"
                     /translation="PQGRPPGITPAEERSGQILLCQARALSDLVVEARLIASVGEVDI
                     KTLPCRIAQLTLLAPDVMQVFLRLPAVERLRFQPGQYLDILLEGDRRRSFSIASPPHD
                     SELLELHVRRVAGGGFTEQLFNANALGTLLRIEGPIGQFIYRPGTSALLMIAGGTGFA
                     PLKSMLRHILEHGIERPIHVYWGARTSQDLYEEARLLEWARRYPQLQFTAVLSEASQT
                     GAAHHRLGWVHEQVLEDHPDLQARDIYAAGPPAMIEAIRASFPGRGASVDRLYFDSFD
                     YAPR"
BASE COUNT         1006 a         1932 c         1959 g         1064 t
ORIGIN      
        1 ggaagttctg ctccgcgtcc tcgcggagtg tgcgcaggcc gggcggcagg ctctcgagca
       61 gccgccgtgc gatctcatct atccgaaggg agtccatgac attacctctc gctagtgaaa
      121 caggctggtg gcagctcgcg ctgcgccctg cgcgccgcgg tgtaactgcc gttcgcgttg
      181 ccccgatatg acgcagaggg aagcagcgaa ggcgccaaaa cggtgcgacc gcggcgcacg
      241 cccgcacgca agtcgtgcaa ctcaatgaat atgccccgtg gcatggaatc tgcactctga
      301 aggcgcccgt cgcaagccca cgcccctcaa cgtcgcgacc gccggagaaa agatgcgcgc
      361 cgctccaacg acttgcctgc tcggatcgag tctcgccctg ctcggaccgg cgatcgcctg
      421 gggggccgat gcccagccgc tgcccgacaa gggcgacacg gcctggatgc tcacctgcac
      481 tgcgctcgtg ctgctgatgt ccgtgccggc gctcgctctg ttctatggcg gtctcgtgcg
      541 gaccaagaac atgctctcgg tgctcatgca ggtgttcgtg ggcttctcgc tcatcaccgt
      601 cctgtggtgt gtatacggct actcgctcgc cttcacggcc ggcaacgcgt tcctgggcgg
      661 attcagccgc gtgttcctgg caggcacgtt cgatcccgcc acccagaggt tctcgatggc
      721 ggccactttc agcaggcaca ccccgctcta cgagctcgtg tatgtggcat tccaatcgac
      781 gtttgcggcg atcacctgct gtctgattct cggctcgatc gtcgaacgca tccgattccc
      841 ggccgtgctg gtgttcctgg tgatctggtt caccttcagc tactgcccca tcgctcacat
      901 ggtgtggtac tcgcctggtc cggatgctta cacgccggac gcgaacgcgg atgcagtgag
      961 tgcgaccggc ggactgctct ggcaatgggg ggcgctcgat ttcgccgggg gtaccgtcgt
     1021 gcatatcaac gcaggggtgg ccgggctcgt gggtgccgtg ctgctgggcc ggcggaccgg
     1081 gttcggacgc gagccgatgc ctccgcacag cctgaccatg acgatgatcg gcgcctcgct
     1141 gctgtggttc ggctggttcg gcttcaacgc cggctccgcg ctcgaggcca acggctcggc
     1201 ggcgctcgcc tttctcaaca cctatctcgc aaccgcgtgc gcggtgctct cctggatgtt
     1261 ctgcgagtgg acactcaagg ggaagccatc gatgttggga gcggcctcgg gcgcggtcgc
     1321 gggtctggtg gccatcacgc cggcggcggg caacgtcggc atcccgggcg cattcgcgat
     1381 cggcattggc gccgggctgg tctgtctctg gggcgtgacc ggtctgaaga gactgatccg
     1441 cgccgatgat gccctggatg tcttcggcat ccatgcgctc ggcggcatct tcggcgcgct
     1501 catgaccggc atcttcaatg ccccggcgct gggcggaccg gggtccgtgc aggactgggc
     1561 caaggggacg gtcgggtacc cgggaatctg gacacagctg cgcatccaga tggaggccgt
     1621 ggggctggtc gtgctctgga cggccgtggt ggccgggctc ggtttcacgg tggtcaaggc
     1681 cctgatgggc cttcgggtga cggaagaaga ggagcgcgag ggcctggata tcacctcgca
     1741 cggggagcgc gcttacgata tgtgaccggc ttcaatagcc gccgtcccgc caaccggcga
     1801 tactgccgag gctggggagg ccctctctta tgttgcgaag catcgcgatc ggcacattga
     1861 tcctcgcagg tatggcggca accgcctttg ccgacgttta ccgctgggtg gacgagcatg
     1921 gggtcccgca ctacaccgat cagtgggtcc ccggtgcaca ggtcatcaag tccagcaagc
     1981 cccaccccgc gacggccgat tcggattccg gcgatcgcaa ctccagcctg caggccggcg
     2041 ccgagcgcgc ctcggagctc atcacgcagc agaatgcgac gcaggccgtc aaggcggacg
     2101 aggccaagat acgcgcgcag cagtgcaagg aagccaagga ccgctacgag aaatcgctca
     2161 tcgcgaggcg cttctacaaa caggccaagc cgggcgagaa agacgacgac aagaccgccg
     2221 agcgcgcgta cctgtcggac caggagatcg aggctttccg cgttcaggcc cgcacggaga
     2281 tggaacaggt ctgcgggaag cagcctgccc cgtagtctcc ctgtccacgc atccggggac
     2341 gctctatagt tggactgcgg ctcactcggc cgccggctac ggatgattcc cacatgcgcg
     2401 taggcttcgc cggcctcggt gccatgggcg cgcacatggc gcgcaatctt catcgcgccg
     2461 ggctgctggg tgcagtctgg aacaggtccg ccgagaaggc tcgctctctg gctgccgagc
     2521 tgggctgcga ggcgccccag actctcgcgg cgctggcctc atcggttgac gcggtcgtca
     2581 tctgtgtctc ggccgacaat gacgtcctgg aagtcgtccg cggcctcgcg ccggagcttc
     2641 gaacgggcgc cctcgtcatc gactgctcca ctgttgccgc cgaaaccgcc cgcagcgcgg
     2701 ccgcactgtt gcacccgagt ggcgtggagt ttctcgattg tcctgtcagc ggaggcgtgg
     2761 agggtgcgca gaacgccacg ctggtcatca tggttggcgg cacacccgag gctttcgagc
     2821 aggcccagcc ggtcctcaag gctatgggca agaccgtcac gctgttcgga cctaccggca
     2881 gtggccaggc tgccaaggcg accaaccaga tcatgtgcgc cgggatcatc gaagcggtcg
     2941 ccgaagcgat ggccttcgca cacgcgcaag gcctgccact cgagagactg atcgatacgc
     3001 tgggcaaggg cgccggctcg agctggtact tcgtacatcg ggcgccgaac atgatgcgtg
     3061 gcagctatcc ggcaggattc cgggtgcggc tgcatgccaa ggatctggga atctgccgcg
     3121 acatggcggc gcgtttcggt gtggcgttgc cggtggtgga gcgcatgctc ggcgagtatg
     3181 ccgaactcgt ctcccgcggc tacggcgacg aggatatctc ggccaccttc cgcctgaagg
     3241 aggagctgtt ccagcgtccg gcaaagccca gcgcgacccc ccgcgacgca tgaagacgga
     3301 accggccgct ggctccgaca aaccgttcta tctaccggat ttctgcacct cgcgagctgc
     3361 actcgcgatc gtcctgatcg tcgagctgac agccctcatg ctcacgctcg cgcgcgatag
     3421 tgcgctgatc ggcttctgga ccgatctggc ccgcacttcc cttttccttt tgtggatcgg
     3481 gctcgccggc gccggattgt tgtgctggct gggtgaaccg ctgaagcggc tgccggtcgc
     3541 acagggatcc gcagctgtgc tcgcgctcat caccggcctc atcaccgtcg tttccctcgt
     3601 cacgttcgtt ctcggccgct cgactttcgg cggctacgcc agcatcgtcg ctctgtttcc
     3661 atccgagccc ggtacgttcc tgtggcgtaa cgtcatcatc gggttcgtcg tgacggccct
     3721 cgcgctgcgt tacttctatg tcactcacga gtggcggcgc aacatcgaga tgcaggcgac
     3781 cgcccgcgtg catgccctgc aggcgcgcat ccgcccgcat ttccttttca acagcatgaa
     3841 caccatcgcc tcgctcaccc ggacccaccc cgaggtcgcc gagcaggcgg tgcaggatct
     3901 cgctgacctc ttccgcgcaa atctgagcga caagcgcgat accatcacgc tcgaggaaga
     3961 atgcgaggtg gcgcggacct accagcgcat cgagcagttg cgtttggggt cgcggttgcg
     4021 catcgactgg aagatcgatc ccctgccgcg caacgccctc gtccccggcc tcatgttgca
     4081 gccgctgctg gagaacgcca tctatcacgg tatcgagccg cagcccgagg gcggcaccgt
     4141 gacggtcacc ggtgagctcg ccggcgggtt gatcaccatc gtggtgcgca atccggtggg
     4201 cgcccagcag aatgagcgcg agggaaatcg cctggcgctg gcgaatatcc gcgagcgtct
     4261 cgggctgatg tacggcgagc ggggcctggt gaaatcggga cgattcgggc ccgagtacat
     4321 cgtgaccttg agatttccgc acatcgagag tcgcgtgaag ctggcaacat gaagctcaag
     4381 gtgctgatcg tggatgatga agcgccggcc cgcgagcgcc tggccagcct gctggcggag
     4441 ttggagggtg tcgagatcgc tggtgaggcg gtcaatggtg tacaggcttt gcagcgcacg
     4501 accgagctga cgcccgacgt cttgctgctg gacgtgcgca tgccgggcat ggacggcctc
     4561 gaggccgcgc gccatctgaa cgcgctcgat gagccgcctg cggtcatctt caccaccgcc
     4621 tacgatgagt acgcgatcaa cgccttcgag gcacaggcag tgggatatct tctcaagccc
     4681 attcgcaagg agaagctcgc cgcggcgctc gcacaggcag gccggctcac ccggccgcaa
     4741 ctgcagaaac tctcggcagg cactcccgaa cggcgcacgc acattgccgc gaagcaccgc
     4801 gaagggctgc ggctgatccc gctggaggac gtgcagtact tcctcgcgga acagaaatac
     4861 acgaccgtca agcatctgaa gggtgaggac ctcatcgagg actcgctccg cgcgctcgag
     4921 gaagaattcg gctcatcgtt cgcgcgcgtg catcgcaatg cgctgatcaa tgtgcgctac
     4981 ctcgaacgga tcgatcgcaa tgccgagggc cagtactgcg tcagactgca gggatgcgag
     5041 gcaccgttgc aggtcagccg gcgcatggcc agcgagctgc gggagcgctt caggatgtga
     5101 gcgatttttc caattcacta ccggggcgcg tagtcgaagg aatcaaagta caggcgatcg
     5161 accgatgcgc cgcgccccgg aaagctggcg cggatggcct cgatcatggc cggtggaccg
     5221 gcggcataga tgtcgcgcgc ttgcagatcc ggatgatcct ccagcacctg ctcgtgcacc
     5281 cagccgagcc gatgatgggc agcacccgtc tgggaagcct cagacagcac ggcggtgaat
     5341 tgcagctggg gatagcggcg cgcccactcg agcagccgtg cctcttcgta aaggtcctgt
     5401 gaggtgcgtg ctccccagta gacatggatc ggtctctcga tgccgtgctc caggatgtgc
     5461 cgcagcatcg acttgagggg tgcgaagccc gtgcccccgg cgatcatcag caacgccgag
     5521 gtccccggcc gatagatgaa ctgcccgatc ggcccctcga ttctcagcag cgtgcccagg
     5581 gcgttcgcgt tgaacagctg ctccgtgaag ccgccaccgg caacacgccg gacatgcagc
     5641 tccagcagct cgctgtcgtg cggtggactg gcgatggaaa aactgcggcg ccgatcgccc
     5701 tcgagcagga tatccagata ctgaccaggc tgaaatcgca gccgttccac tgccggcagg
     5761 cgcaggaaga cctgcatgac atcgggcgcc agcaatgtca gctgcgcgat gcggcagggc
     5821 agtgtcttga tgtccacttc gccgacactg gcgatgagcc gggcttcgac cacgaggtcg
     5881 ctcaacgccc gcgcctggca cagcaggatc tggcctgatc gctcttcggc tggagtgatg
     5941 ccggggggac gaccttgcgg a
//
LOCUS       DASRHN010000264         8572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1246851, whole genome shotgun sequence.
ACCESSION   DASRHN010000264 DASRHN010000000
VERSION     DASRHN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246851"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFB37_10435"
     CDS             complement(<1..1015)
                     /locus_tag="VFB37_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22905.1"
                     /translation="MRKRCLHVILTGLAFAALPLASLAGVSVGISVNIAPPALPVYTQ
                     PVCPGVGYIWTPGYWAYGDDDYYWVPGTWVMAPSPGLLWTPGYWGWDGGVYLWHGGYW
                     GPHVGFYGGVNYGYGYGGVGFEGGYWRGGAFYYNRSVNNLGGIHVTNVYNKTVINNIT
                     VNRVSYNGGSGGLTARPTAREVAAEHEHHVEFTPAQREQQHLASTNRDLRASVNGGKP
                     RIAATSRPGVFSGRNVVAARAAGGPVHAGETHAVRNDRPPNATGQHGAGEAALHPGET
                     VHNAPSQAMMRNERPASHASAATTRPVQHAAPPVSARERPAIEHAPIERGPAERPAVE
                     HAPA"
     gene            1340..3583
                     /locus_tag="VFB37_10440"
     CDS             1340..3583
                     /locus_tag="VFB37_10440"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZO22906.1"
                     /translation="MKWGALVLLALLVALLVICAILERNADALRGPISRSLSHHLNRP
                     VRIDGRLTLHLLSLEPSIVVKDIHVANPAWAQLREMARIGQLTASLSVPDLLRAELVV
                     PHLEIDDADVNLLRDASERANWDFGGPPQPSSSQPTRLPVVRSLRIDGGHLLVQDAIR
                     KLKFDGAVRASQGGGATTLQLDGNGNINGATFKMFAHGDPLFTADRSKPYSFQADVQA
                     GATHVSARATLTKPFDLGALDTEFTITGDDLADVYYLTSLALPNTAPYSLTARVKREG
                     NRVIVSDLRARFAKSDLSGQATIDIGGARPVLTADIDSRSLDLVDIAPSLGAPTKTAR
                     PGQGSLATGQQSVSTAPAEKGPTAVQPAQKDATKEDSTKEDSTKGSAQKGSTKEGAIQ
                     QASTQQDATPQQSTQSEPTQKNSTHEKLGKRDEGKTAAKQSEAAAAGGLLLPDAHLQV
                     ERVRGMDATVHYQALTVVAQKVPIRHLDFTLRLKDGVIDIQPLSLSLPQGKISGTVRI
                     DASRDVPDVTLDMRLNSIQLSQFRPRNSAPLATGTLVGRVQLHGHGSSVHDAAATADG
                     IVTVAVPHGEVREAFAELTGIDIARGLGLLLAHNQQETGIRCGVANFQAQNGVLNAKD
                     IVFDTDTVLVTGKGDIDLRNEKLNLTMNGQPKKFRFFRIKSPIALGGTLRKPSIGLKP
                     GNTPAQAGVAVALGTLLTPVAAALAFIDPGLAKSADCSALLDEAKGHGAPVKQAEVSN
                     AARTPSR"
     gene            complement(3555..4331)
                     /locus_tag="VFB37_10445"
     CDS             complement(3555..4331)
                     /locus_tag="VFB37_10445"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004880003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HZO22907.1"
                     /translation="MAAIGTERVLHVHHWTDTQFTFSTTRDSGLRFENGQFVMVGLQV
                     NSRPLLRAYSIASANHEEHLQFLSIKVPDGPLTSRLQHIRRGDAILVSRKPTGTLVLH
                     DLKPGRRLYLLSTGTGAAPFMAVIKDPEVYERFEHVTLVHGVRWSRETSVVKHLLDEL
                     RQHEYLGESVREKLHYYPTITREPHPNHGRLTALIRSGKLFEDLHLPEFQPGEDRVMV
                     CGSPGMLLDTCTLLDSRGFRISPHIGEPGDYVIERAFVQR"
     gene            4458..5306
                     /locus_tag="VFB37_10450"
     CDS             4458..5306
                     /locus_tag="VFB37_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO22908.1"
                     /translation="MNDPAAGSLHGRTLFITGASRGIGLAIALRAARDGANVAIAAKT
                     TEPHPKLPGTIYSAAEEIERAGGRALALAVDVRHEEQVEAAVAKTVETFGGLDILVNN
                     ASAISPTGTAQTDMKRFDLMQQINTRGTFLCSKLCLPHLARSPNPHILMISPPLNIEE
                     RWFAPHLAYSLAKYGMSLCVLGLAGELRAQGIAVNALWPRTVIDTAALQIALAGRPAA
                     ERRRVRKPQIMADAAHVILTSDSRQFTGRFCIDEEILRAAGHTDLSRYRHEGVSEEDL
                     LPDYFL"
     gene            complement(5361..5990)
                     /locus_tag="VFB37_10455"
     CDS             complement(5361..5990)
                     /locus_tag="VFB37_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZO22909.1"
                     /translation="MSRSIRPAFLRRNPLGLILVMGLHVTFLWALIAGLRIHPPRLAP
                     DSVFTRFLPPELARPLPPPEVVQPAHLATPLETLLPQVQFPEDPPPDAPNASPTEVPL
                     EATGAVAGHEAVLTTPSIDPRSPLTQPPYPAAAIRAGIEGNLALQVLVGPDGRVHDAR
                     VVRTSGSALLDQAAIDEALRRWKLRPATRDGVPFEQWYTLRVIFHLQNR"
     gene            complement(6067..6804)
                     /locus_tag="VFB37_10460"
     CDS             complement(6067..6804)
                     /locus_tag="VFB37_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO22910.1"
                     /translation="MSVEFDQIQMEPAYRKVASAIGERILDRRLSEGERLPAETELAR
                     QFGVNRSTVREALRELESHGLVMRRRGSRRLSVSRPPHLAIAEGVSRALVLHDVTYLE
                     VWEALTILEPPIAEAAARARTADDLEMITSAAEQFTAGQLETVRAVQLAAKFFRCVGR
                     ATHNQVLVLAQEPLLQLLEPSLQVMIDKAPQARSRITTAQRRICEALKARDAALAGSW
                     MAKHIRDFRKGFEIARIDLEHRVTPAE"
     gene            complement(6801..7964)
                     /locus_tag="VFB37_10465"
     CDS             complement(6801..7964)
                     /locus_tag="VFB37_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HZO22911.1"
                     /translation="MGTLDCDFGLDETLEEIRQQVRRFAAERIAPRAAEIDRSNEFPR
                     DLWPALGEQGLLGVTVPERWGGAGLGYLAHVLVMEEISRASGALGLSYGAHSNLCVNQ
                     LRLNGSDAQRDRYLPKLVSGEHVGALAMSEPGSGSDVVSMQLRADKRGDSYVLNGRKM
                     WITNGPDADVFVVYAKTDPAAGSRGISAFIIERGSAGFTTAQKLDKLGMRGSGTCELV
                     FEDCHVPAGNLLGGENKGAAVLMSGLDYERVVLAGGPLGLMAAALDLVLPYVRERKQF
                     GQPIGTFELMQAKVADMYAALNASRAYVYTVARACDKKSVKRRDAASCILFAAEHATQ
                     AALEAIQALGGNGYTNDYGAGRLLRDAKLYEIGAGTQEIRRMLIGRELFESPA"
     gene            7994..>8572
                     /locus_tag="VFB37_10470"
     CDS             7994..>8572
                     /locus_tag="VFB37_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZO22912.1"
                     /translation="MIVGQTGNHGKSMAALSTRIDMHSQEYRDNGAAMRELTADLRRE
                     VARAALGGSAAARKKHREAGKLTARERIGVLLDTGSPFLELSQLAAHGMYGGEIACAG
                     IVTGIGRVSGRECVIVANDPTVKGGTYYPITVKKHLRAQEIARENRLPCIYLVESGGA
                     FLPAQDEVFPDREHFGRIFYNQATLSGSGVAQI"
BASE COUNT         1526 a         2792 c         2778 g         1476 t
ORIGIN      
        1 cggccggggc gtgctcgacg gcgggtcgct cggcaggtcc acgctcgatc ggcgcatgct
       61 cgatcgccgg ccgttcgcgc gcggatactg gcggcgcggc gtgctggacc ggacgggtcg
      121 tcgccgcact cgcgtgcgac gccgggcgct cattgcgcat cattgcctgt gaaggcgcat
      181 tgtgtaccgt ctcgcctggg tgcagcgccg cttcgcccgc tccgtgctgc ccggttgcat
      241 tcggcggccg atcgttcctc accgcatggg tctcgcccgc gtggactgga ccccctgctg
      301 cgcgggccgc gaccacattc ctgccggaaa acaccccggg cctcgaggtg gctgcgatgc
      361 gcggtttgcc gccattgacc gaggcgcgca gatcgcggtt cgtagacgca aggtgctgct
      421 gctcgcgctg cgcaggcgtg aactcgacgt gatgctcgtg ctcggcggcg acctcgcgcg
      481 cggtcggacg tgcggtcagg ccaccggaac cgccgttgta gctcacccgg ttcacggtga
      541 tgttgttgat gaccgtcttg ttgtagacgt tggtgacgtg aataccgcca aggttattga
      601 cggaccggtt gtagtaaaac gcgccgccgc gccagtagcc accctcgaaa ccgacgccgc
      661 cgtagccgta cccgtaattg acgccaccgt agaagcccac gtgagggccc cagtaacctc
      721 cgtgccagag ataaactcct ccatcccacc cccaatagcc gggggtccac agtaatccgg
      781 ggctgggcgc catgacccac gtacccggaa cccagtagta gtcatcatcg ccataggccc
      841 agtagccggg cgtccagatg tatccgacac ccgggcaaac cggctgcgtg tagaccggca
      901 gagccggtgg tgcaatgttg accgaaatgc cgaccgacac accggcgagg gacgccagag
      961 gcaatgcggc gaacgcgagc cctgtaagga tgacgtgaag gcatctcttg cgcatgctga
     1021 cctcgcttgt gtcgtgactg aacgccggct gaacgtgccg acgcaacact ccctgattta
     1081 ctgttcctct catacgggca aacgcaggcc tgcaaacctc gttgaccctg ttgcggcgcc
     1141 gcgaatccac cgctgagtgc tccgcgaggc gccggctgcg tattgtgcat gcactcgggc
     1201 cccggcgcgt gctgcgcaat ccgattccgt gcgctaagca cgcgtgacgc ttcatggcga
     1261 cagaaacaga accgtatccc gattcgcgcg tttagcccgc atgcctgaat ctcaagctgt
     1321 aagccacact ccgcgattcg tgaagtgggg tgcattggtg ctgctggcgt tgctggtcgc
     1381 gctcctggtg atctgcgcga ttctcgagag gaacgcggat gccctgcgcg gacccatcag
     1441 ccgctcgctc agccatcatc tgaatcgtcc ggtgcggatc gacggtcgat tgaccttgca
     1501 tctgctctca ctcgagccct ccatcgtggt caaggacatc cacgtcgcca atcccgcttg
     1561 ggcccagctg cgggaaatgg cgcgtatcgg acagctcacc gcatcgcttt ccgtgcccga
     1621 tttgctgcgc gcggaactcg tcgtaccaca cctggaaatc gatgacgccg acgtcaattt
     1681 gctccgcgat gcatccgagc gtgccaactg ggattttggg ggtccgcccc aaccctcttc
     1741 ctcccagcct acccggctgc ccgtggtccg cagcttgcgc atcgacgggg gccacctcct
     1801 ggtccaggat gcgatccgca agctcaaatt cgacggggcg gtgcgtgcca gccagggagg
     1861 aggcgccacg acactccaac tggacggcaa tgggaacatc aacggtgcaa ccttcaagat
     1921 gttcgcccat ggcgacccgc tcttcacggc cgaccgctcc aagccgtact cctttcaggc
     1981 cgacgtgcaa gccggtgcga cccacgtatc ggccagggcg accctgacca agccgttcga
     2041 ccttggggca ctcgatacgg agttcacgat tacgggagat gatttggccg atgtctacta
     2101 cctgacgagc cttgccctcc ccaacacggc cccctacagc ctgaccgcca gggtcaagcg
     2161 cgaaggcaac cgggtgatcg tgagtgatct gcgtgcccgc tttgccaaga gcgatctgag
     2221 cggtcaggcc acgatcgaca tcgggggtgc gcggccggtc ctgaccgccg atatcgactc
     2281 ccgctcgctc gatctcgtgg acatcgctcc ctccctggga gcgcctacga agaccgcacg
     2341 gccaggtcag ggctcgctgg caacgggcca acagagcgtt tcaacagcac cggcggagaa
     2401 ggggcccacg gcagtccaac ccgcgcagaa ggacgccacg aaggaagact ccacgaagga
     2461 agactccacg aaggggtcgg cgcagaaggg ctccacgaaa gagggcgcca tacagcaggc
     2521 ctcgacgcag caggatgcca cgccgcagca gtccactcag agtgagccca cgcagaagaa
     2581 ctccacgcat gagaaattgg gcaagcgaga tgaggggaaa accgccgcga agcagagcga
     2641 agctgccgca gcgggtggcc tgcttcttcc cgacgcacat ctgcaagtcg agcgcgtccg
     2701 tgggatggat gccacggtgc actaccaggc gctcacggtc gtggcgcaga aggtgccgat
     2761 ccgacacctg gatttcactc tgcgactcaa agatggcgtc atcgatatcc agcctctgtc
     2821 gctgagtctt ccacagggca agatatcggg cacggtgcgg atcgatgcga gccgggacgt
     2881 gccggacgtg acgctcgata tgcgcctgaa ctcgatccag ctctcgcagt tccgaccgcg
     2941 caactcagcc cccctggcca cgggcacgct ggtgggacgc gttcaacttc atggtcacgg
     3001 ttcatccgtg cacgatgcgg cagcaacagc cgacggcatc gtcaccgtag ccgtgcccca
     3061 tggtgaggtt cgtgaggcgt ttgcggagct cacgggcatc gacatcgcac gtgggttggg
     3121 cttgctgctt gcccataacc agcaggagac aggcatccgc tgtggagtgg ccaactttca
     3181 ggcgcagaat ggcgtgctga acgccaagga catcgtcttc gataccgata cggtgctggt
     3241 caccggaaaa ggggatatcg acctgcgcaa tgagaagctg aatctcacga tgaatgggca
     3301 gccgaagaaa ttccgcttct tccgtatcaa atcacccatt gccctcggcg gcacgctgcg
     3361 caaaccctcc atcgggctca agcccggcaa cacgcccgct caggcgggcg tggcggtggc
     3421 gctaggcacc ttgctgacgc ccgtggccgc ggccctggca ttcatcgatc cggggcttgc
     3481 caagagcgcg gactgcagcg cactgctgga cgaggcgaag ggacacggcg cgccggtcaa
     3541 gcaggccgaa gtgtctaacg ctgcacgaac gccctctcga tgacgtagtc gccgggctcg
     3601 ccgatgtgcg gcgagatccg gaacccgcgt gaatcgagca gggtacacgt atccaagagc
     3661 atgcccgggc tgccgcagac catcacccgg tcctcgcctg gctgaaactc cggaaggtgc
     3721 agatcctcga acagcttgcc gctgcggatc aatgctgtga gccttccgtg gttgggatga
     3781 ggctcgcgtg tgatcgtcgg gtagtagtgc aacttctccc ggacggactc ccccagatac
     3841 tcatgctgcc tcagctcatc gagcagatgc ttgacgaccg aggtctcgcg cgaccagcgg
     3901 acgccatgca cgagcgtgac atgctcgaag cgctcgtaca cctcggggtc cttgatgacg
     3961 gccatgaagg gagctgcgcc cgtgcccgtg ctcagcaggt acagccgccg ccccggtttc
     4021 agatcatgca gcaccagtgt gccggtgggc ttgcggctga cgagaatcgc atcgccgcgg
     4081 cggatgtgct gcagccgtga ggtcaacggt ccatcgggca ccttgatgct cagaaactgc
     4141 agatgctcct cgtgattcgc gcttgcgatg ctgtaggcgc gcagcagcgg cctgctgttc
     4201 acctgcaggc cgaccatgac gaactggccg ttttcgaagc gcagcccgga atcgcgcgtc
     4261 gtggagaagg tgaattgcgt gtcggtccag tgatggacgt gaagcacacg ctccgtaccg
     4321 atagctgcca agatagcgtt acctgcctgt ccatgccatg aggcagaatc ttacccgagc
     4381 cgcgggcctc cacgaggctc aggcggcatg gcctcataat ccgcagttat ggtcgcaggc
     4441 atgcgtgagc gacagcgatg aatgatccgg cggcgggctc gctccacggg cgcacgttgt
     4501 tcatcacggg ggcgagccgt gggatcggtc ttgccattgc gttgcgtgcg gctcgcgatg
     4561 gcgcgaatgt ggcgatcgct gcgaagacga ccgagccgca cccgaagctg ccgggcacga
     4621 tctatagcgc cgccgaagag atcgagagag cagggggccg ggctctggcg ctggcggtgg
     4681 atgttcgcca tgaggagcag gtcgaggctg cggtcgccaa aacggtcgag actttcggcg
     4741 gcctcgacat cctggtgaac aatgcgagtg cgatcagccc cacgggcacg gcccagaccg
     4801 acatgaagcg cttcgatctg atgcagcaga tcaacacgcg cggcaccttt ctgtgctcga
     4861 agctgtgcct gccccatctc gcgcgctcac ccaacccgca tatcctcatg atctctccgc
     4921 cgttgaacat cgaggagcgc tggttcgccc ctcatctcgc gtactcgttg gcaaaatacg
     4981 gcatgagcct gtgcgtgctc gggctggcgg gcgagttgcg tgcgcaaggc attgcggtca
     5041 acgcgctttg gccgcgcact gtgattgata ccgcggctct gcagatcgcc ctggcgggac
     5101 gccccgccgc cgagcgccga cgcgtgcgca agccacagat catggctgac gcggcgcacg
     5161 tcatcctgac cagtgacagt cgtcaattca cgggcaggtt ctgcatcgac gaagaaatct
     5221 tgcgtgcggc cggacacacc gacctctcgc gctatcgcca tgaaggcgtg agcgaagaag
     5281 acctgctgcc cgattacttc ctctagctcg cgcacactgg accgatgagc cggcgggccg
     5341 cccgcctctg tgttgatgtc tcaccggttc tgcagatgga agatcaccct gagcgtgtac
     5401 cactgctcga agggtacgcc gtcgcgcgtg gccggccgca gcttccagcg ccgaagcgct
     5461 tcgtcgattg ccgcctgatc aagaagcgcg gagccgctcg tgcgtaccac gcgcgcatcg
     5521 tgcacccgac cgtccggacc gacgagcacc tgcagggcaa ggttcccttc gatcccggct
     5581 cggatggcgg ctgcagggta gggcggctgg gtcagcggtg agcgcgggtc gatcgaaggc
     5641 gttgtcagga cggcctcgtg accggccacc gcgcccgttg cctcgagcgg gacctcggtc
     5701 ggcgaggcgt tcggggcgtc cggtggcgga tcctcaggga attggacctg cggcaataat
     5761 gtctcgagag gcgttgcaag gtgagcgggc tgcacgacct cggggggcgg cagcggtcgg
     5821 gccagctcgg gcggcaggaa ccgggtgaac acggaatcgg gtgccaggcg tggcggatga
     5881 attcgaagcc cggcgatcaa agcccacagg aatgtgacgt gcaatcccat gacgagtatc
     5941 aagccgagcg gattgcgccg caggaacgcg ggtcggatcg aacgtgacat gcgacctccc
     6001 ttggccgggg atagctgctc ggttgactat caacggacca gcgggccgtg gagggttagc
     6061 tttggatcac tcggctggcg tgacgcgatg ttcgaggtcg atccgcgcga tctcgaagcc
     6121 cttgcggaag tcgcggatgt gcttggccat ccagcttcca gcaagtgctg catcgcgcgc
     6181 cttcaacgct tcgcagatgc gacgctgcgc tgtggtgatg cgcgagcgcg cctgcggggc
     6241 cttgtcgatc atgacctgca atgagggttc cagaagctgc agcaacggct cctgggccag
     6301 gaccagcacc tggttgtgcg tggcgcgccc cacgcagcgg aagaacttcg ccgcaagctg
     6361 caccgcccgc accgtctcga gctggccggc ggtgaactgc tcggcggcgc tggtgatcat
     6421 ctccagatca tcggctgtcc gagcccgggc ggccgcctcc gcgatgggcg gctcgagaat
     6481 cgtcaaggcc tcccacacct cgaggtaggt cacatcatga agcaccaggg cgcggctgac
     6541 gccctcggct attgccagat ggggtggcct gctgaccgag agtcgacgcg agccgcgccg
     6601 ccgcatgacc aagccgtggc tctccagctc ccgcagcgcc tcgcgcaccg tcgagcgatt
     6661 gaccccgaac tgacgcgcca gctccgtctc cgcgggcagg cgctcacctt cgctgaggcg
     6721 ccgatcgagt atccgctcgc caatggccga ggcgaccttc cgataggccg gctccatctg
     6781 gatctgatcg aactcgacgc tcacgccggc gactcgaaca actcgcggcc gatcagcatg
     6841 cgccggatct cctgggtgcc ggccccaatc tcgtagagct tggcgtccct gaggaggcgt
     6901 ccggctccgt agtcgttggt gtagccgttt ccaccgagcg cctgaatcgc ctcgagcgcc
     6961 gcctgcgtgg catgttcggc ggcgaacagg atgcagctcg cggcatctct tcgcttgacg
     7021 ctcttcttgt cgcacgcgcg cgcgaccgta taaacgtacg cgcggctcgc attgagcgcg
     7081 gcgtacatgt cggccacctt cgcctgcatc aactcgaatg tgccgatggg ctgaccgaac
     7141 tgcttgcgct cgcgcacgta gggcagaacc aggtcgagcg ccgcggccat gagccccagc
     7201 gggccgccgg cgagcaccac gcgctcgtag tccaggccgc tcatcagcac ggccgcgcct
     7261 ttgttttccc ctccgagcag attacccgcc ggcacgtgac agtcctcaaa caccagctca
     7321 caggtcccgg agccgcgcat gccgagcttg tcgagcttct gtgcggtggt gaagcccgcg
     7381 ctgccgcgct cgatgatgaa agcgctgatg ccgcgcgaac ccgcggccgg atcggtcttc
     7441 gcgtacacca cgaagacgtc ggcatcgggg ccgttggtga tccacatctt gcgcccgttg
     7501 agcacgtatg aatcgccgcg cttgtccgcg cggagctgca tcgagacgac atcggagccg
     7561 gagccgggct cggacatcgc cagggcgccc acatgctcac cgctgacgag tttgggcaga
     7621 tagcggtcgc gctgcgcatc gctgccattg agacggagct ggttcacgca caggttcgaa
     7681 tgcgcgccat aggagaggcc gagggctccg gaggcgcgtg agatctcctc catgaccagc
     7741 acatgcgcaa gatagcccag gcccgcgccg ccccagcgct cgggcaccgt gacgccgagc
     7801 agcccttgct cgccgagggc aggccacaag tcgcggggga attcgttgct gcggtcgatc
     7861 tcggctgcgc gcggtgcgat ccgctcggcg gcgaagcggc gcacctgctg gcggatctcc
     7921 tcgagggtct catcgaggcc gaagtcacag tcgagagtgc ccatgcgctt gccttcccat
     7981 tctgtaggat cgtatgattg tcggacaaac cggcaaccat ggcaagtcga tggccgcgct
     8041 gtccacccgg atcgatatgc attcgcagga ataccgcgac aatggggctg ccatgcggga
     8101 gctgacggcg gatctgcgcc gcgaggtcgc ccgggcggcg cttgggggct ccgccgccgc
     8161 ccggaagaag catcgcgagg ccggtaagct cactgcgcgg gagcgtatcg gcgtgctgct
     8221 ggatacgggc agtccgttcc tggagctctc gcagctggct gcccatggaa tgtatggggg
     8281 tgagatcgcc tgcgccggaa tcgtgacggg catcgggcgt gtcagcgggc gcgagtgcgt
     8341 gatcgtcgcc aacgatccga cggtgaaggg cggcacgtac tatccgatca cggtcaagaa
     8401 gcatctgcgc gcacaggaga tcgcccgcga aaaccgcctg ccctgcatct atctggtgga
     8461 gagcggcggg gctttcctgc ccgctcagga tgaggtcttt cccgaccggg agcacttcgg
     8521 gcgcattttc tataaccagg ccacactctc cggctccggc gtcgcacaga tt
//
LOCUS       DASRHN010000265         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_758672, whole genome shotgun sequence.
ACCESSION   DASRHN010000265 DASRHN010000000
VERSION     DASRHN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758672"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /gene="flgA"
                     /locus_tag="VFB37_10475"
     CDS             <1..769
                     /gene="flgA"
                     /locus_tag="VFB37_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HZO22913.1"
                     /translation="GMIDSVTANRHFRGARAALRAGWGALALMGLWSGPSSAQTSTAA
                     PAAESLATIRAAAQSCVKGLLPPSPGGNTVTVGQLDSRLRLPHCSQALSAALPPGMPL
                     QARMTIGVTCAGANHWTVYVPVTVESTINVLVLSHAVGRDSRLTVADVMTEMHKTAGP
                     GTAYLSSPTELSNRTVRRPLAAGTLLTADMFAPDLIVRRGQEVTLLSSGDLIEVRASG
                     RAMSDGAAGARIQVQNLSSLRVVEGVVETADLVRIAR"
     gene            843..1166
                     /gene="flgM"
                     /locus_tag="VFB37_10480"
     CDS             843..1166
                     /gene="flgM"
                     /locus_tag="VFB37_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZO22914.1"
                     /translation="MAVNLTGIELNGPTLGQARKTSTSSGGASSGAQENQADSGEVQI
                     TSTAALLAQLEQSLARQPAVDANRVAALSQAIASGRYQVVPEQVASGLIGAERSLAAL
                     PLAEI"
     gene            1170..1655
                     /locus_tag="VFB37_10485"
     CDS             1170..1655
                     /locus_tag="VFB37_10485"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HZO22915.1"
                     /translation="MEAGLCREKLGALMAQETHLLEELSRLLEKEHEYLVASEVVSLE
                     EASRERQRCVARILALDEERRVLCLASGRTHDAKGVQELLRWCDPQGTLAAVWAKCLA
                     VAASCRKLNDRNGALVGARLKHVQERLGVLIESRRETVTYGARGAHATGGSGRVLATK
                     A"
     gene            1781..2110
                     /gene="flgM"
                     /locus_tag="VFB37_10490"
     CDS             1781..2110
                     /gene="flgM"
                     /locus_tag="VFB37_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZO22916.1"
                     /translation="MAGKISGVDGLQSASVGAGTAVPRSKDAAGGETSTADSSDVQIT
                     GTARTLAALEQTLRDVPPINEARVAALRTAIEQGTYAVRPEQIADQLTQLERALGGLP
                     QQGEPAE"
BASE COUNT          373 a          694 c          758 g          335 t
ORIGIN      
        1 cggcatgatc gacagtgtga cggctaatcg acacttcagg ggagcacgcg ccgccttacg
       61 tgcagggtgg ggcgcgctgg ccctcatggg cttgtggtca gggccttcct cggctcagac
      121 gtcgacggcc gccccggcag cggaatctct ggcaacgatc cgtgcggcgg cccagtcctg
      181 tgtcaaagga cttctgccgc cgagtcctgg cggcaacaca gtcacggtgg ggcaactcga
      241 cagtcgtttg cggctgccac actgttctca ggcgttgagt gcggccttgc cacccggaat
      301 gcctctgcag gcgcgcatga ccatcggggt gacgtgcgcc ggggcaaacc actggaccgt
      361 gtatgttccg gtgacagtcg agagcactat caatgtgctc gtgctgagcc acgcggtagg
      421 gcgcgactcg cgcctgaccg tggcggatgt catgaccgag atgcacaaga ccgcagggcc
      481 cggaaccgcc tatctgagca gcccgaccga gctgagcaac cggaccgtgc gccgtccgtt
      541 ggctgcgggg acgctgctca ccgctgatat gtttgcgccc gatctgatcg ttcggcgtgg
      601 ccaggaggtg acgctgctct cctccggcga tctgatcgaa gtgcgggcga gcgggcgagc
      661 gatgagcgac ggtgccgcgg gcgcccgcat ccaggtacag aacctgagct cgctccgggt
      721 cgtcgaaggc gtcgtcgaga cggccgacct ggtgagaatc gcccggtaaa aatcgtgagc
      781 acgctctcaa gttcggcggc cgcgggccgc tatgcaagga tctcgaagca ggagggccac
      841 gcgtggccgt caatctgact ggaattgagc tgaatggtcc gacgctgggg caggctcgca
      901 agacctccac ctcatcgggc ggcgcctcct cgggcgcgca ggaaaatcag gccgactcgg
      961 gcgaagtgca gatcaccagc accgccgcgc tgctcgcaca gctggagcag tccctggcga
     1021 ggcagccggc agtggacgcg aaccgcgtgg ccgcactcag ccaggcgatc gcgagcgggc
     1081 gctaccaggt ggtcccggag caggtggcct ccggcctgat cggcgcggaa cgctccctgg
     1141 ccgcgctgcc gctggcggaa atctaggcca tggaggccgg cctgtgccgt gaaaagctcg
     1201 gcgccctgat ggcgcaggag acgcatctgc tcgaggagct gtcgaggctt ctcgagaagg
     1261 aacacgagta cctcgtcgcg agtgaggtcg tctcgctcga ggaggcctcg cgcgagcggc
     1321 agcgctgcgt agcccgcatt cttgcgctgg atgaggagcg gcgggtgctg tgcctcgcat
     1381 caggacgcac tcatgatgcc aaaggcgtcc aggagctgct gcgctggtgc gatccgcagg
     1441 gcacgctcgc tgccgtctgg gccaaatgcc tggcggtcgc tgcgagctgc cgcaagctca
     1501 acgaccgtaa cggggcactc gtcggggccc gcctcaagca cgtgcaggag cggctcggtg
     1561 tcctcatcga gagtcgtcgt gagacggtca catacggcgc ccggggagcg cacgcaaccg
     1621 gcggatccgg ccgggtgctc gcgaccaagg catagccaga gctctggatg gcccggagcc
     1681 ttcagcgggc ctgcgcaaag tgtgacgcag gtctcatcta aagttcctcc atcgccggcc
     1741 gatacagcct cagaggtact tcctctcgaa ggagaatccc gtggccggca agatcagcgg
     1801 agtcgatggc ctccagagcg cctcggtggg tgctgggacg gccgtgccgc gatccaagga
     1861 cgcggcgggt ggcgaaacct ccacggcgga ctccagcgat gtccagatca ccggaacggc
     1921 gcgcactctt gcggccctag agcagacgct gcgcgacgtt ccccccatca acgaggcgcg
     1981 agtcgcagcc ctgcgtaccg cgatcgagca gggaacctac gcggttcgcc ccgagcagat
     2041 cgccgatcag ctcacgcagc tggagcgcgc tctcggaggc ctgccccaac agggtgagcc
     2101 tgcggagtag agtgtggagc gggatgccag tcgcgagtgc ctcgccagct tgctgagcga
//
LOCUS       DASRHN010000266         9259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2232983, whole genome shotgun sequence.
ACCESSION   DASRHN010000266 DASRHN010000000
VERSION     DASRHN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232983"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFB37_10495"
     CDS             complement(<1..967)
                     /locus_tag="VFB37_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO22917.1"
                     /translation="MEPTDTSHPDYYHRVVDCQWACPAHTDVPEYIRLIAQGRFTDAY
                     MVNRDSNVFPGILGRVCDRPCEPACRRARVENKPVAICRLKRVAADLREDVAGLLPKI
                     PKAKNGRRIACIGAGCASLTVANDLMPLGYQVTIFEQFAEPGGLMRTNIPSFRLPHTV
                     LNEEIAMITDMGVDLKLNHPISSMRELLATGGFDAVFVGSGAPKGKELKLPGRAEGAA
                     HIHIGIAWLESVAFQHIDRIGERVLIIGVGNTAMDCCRTSLRLGARSVKVMARKPRGF
                     FKASAWELEDAEEESVEIIVNRSPKAFVLENGRLTGMTFDRMEYEL"
     gene            1145..1495
                     /locus_tag="VFB37_10500"
     CDS             1145..1495
                     /locus_tag="VFB37_10500"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZO22918.1"
                     /translation="MGDPIATEIRVRSRSRLLEVSFQDGSRFELPFEFLRVHSPSAEV
                     KGHGPGQETLVLGKEQVGIRAVEPVGQYAVKLVFDDGHDTGLYTWTYLHELGSNQAVL
                     WQRYLQRRAQATGS"
     gene            1558..2304
                     /gene="ubiE"
                     /locus_tag="VFB37_10505"
     CDS             1558..2304
                     /gene="ubiE"
                     /locus_tag="VFB37_10505"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HZO22919.1"
                     /translation="MADPFTDFGFERIPWREKARRVRGVFDSVTSRYDLMNDLMSGGI
                     HRLWKRFTLTLANLREGQSALDLAGGTGDLAAGLAQQVGSRGIVVLSDINASMLARGR
                     DRLIDAGRSGNLHYVQANAERLPFASHSFDCVTIGFGLRNVTDKNAALAAMQRVLKPG
                     GQLLVLEFSHPVMPGLKPLSDAYCMRVLPMLGKLVVGDESSYRYLAESIRMHPPQETL
                     LQMMRDAGLEGCRYHNLSGGIVAVHRGYKS"
     gene            2307..2930
                     /locus_tag="VFB37_10510"
     CDS             2307..2930
                     /locus_tag="VFB37_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZO22920.1"
                     /translation="MQRMLAETLQNLLNRGLPRSPRAQQLCAELAGRSVAVELRDIFS
                     VRVESTGATLSVTRPAGPADARICGGPLGLLALAGDSPQAVLQRGQARIEGDSEVAGK
                     FRELGQLLRPDLEEELALLLGDIPAHQIGRFARAALNWSRNAASTMADNFAEYLAHEQ
                     GDLVSRGEANQFLRGIDALREDVDRLAARIDLLQQRPRTRSAEQHDS"
     gene            2969..4612
                     /gene="ubiB"
                     /locus_tag="VFB37_10515"
     CDS             2969..4612
                     /gene="ubiB"
                     /locus_tag="VFB37_10515"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HZO22921.1"
                     /translation="MRLRVLARLIQIHRVLLKHGLDDLVRATHLYRPLRFLFYFSPGI
                     WFQRSRRAERGERLRLALQELGPIFVKFGQAVSTRRDLLPTDIADELARLQDRVPPFP
                     GNVARQIIERAHGRPVTETFSEFDEEPLAAASIAQVHAAKLADGTEVVVKVLRPGMRE
                     MIEQDLEVLYSLAALAHRYWAESRRLRPLEVVSEYEKTILDELDLMREAANASQLKRN
                     FLGSDLLYVPEIYWDYCRVNVMVMERIRGIQISDRERLYAAGTDIAQLAENGVKIFFT
                     QVFRHNFFHADMHPGNIFVIVDEPRRPRYAAVDFGIVGTLDPRDQHYLAENFLAVFDR
                     DYQRVARLHLESGWVPPDSRLDEMESAVRTVCEPIFDRPLQDISFGQILLRLFEISRR
                     FNMQVQPQLILLQKTLLNVEGLGRDLYPQLDIWKTASPILRQWMRERMSVGQLLKNLR
                     RDLPELMETARVLPSLVKAFAHRAQGGVLRMEVESQALDALHTRIRNVGRRRDAITIG
                     AAILLGGLVWLALSRGGHWPGWILTLAGTVWLLVASRLP"
     gene            4671..6074
                     /locus_tag="VFB37_10520"
     CDS             4671..6074
                     /locus_tag="VFB37_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZO22922.1"
                     /translation="MQNPRSLTEGSISRALLQFSLPILYANVLQSLNGSVNSIWVGRF
                     LGEAALTATSNANTVMFLLIGAAFGIALAATILIGQTIGANDIAHAKRVVGTSATFFA
                     AISIAMAVAGLMLCRPLLIAMKTPGDSLQLAVDYMRVIFLALPCLYMYAFVMSVLRGA
                     GDSKTPFYFMLLSVAIDIALNPVFIFGLGPAPRLGIAGSALATFMAQAISLTALIVHL
                     YRRRHLLVLHKSEAALLRIDWVIVGALVRKGIPMSAQMLVLSLSGVLMITLVNRFGVD
                     TTAAFGAALQLWNYIQMPAFAVGMGVSAMAAQNVGAKKWDRVNSIARVGVLYSVLLTG
                     SVILVLEVLNRHAFSLFLVEDSRAMLAASHLNRIATPGFVFFGISMVLFGVVRATGAV
                     MAPLICLTVSLLLVRVPLADLLLGRLHVDAIWWSFPISSALAAVLAVLYYKFGGWRSA
                     TMERPPKSEPVHPVEET"
     gene            6150..7259
                     /locus_tag="VFB37_10525"
     CDS             6150..7259
                     /locus_tag="VFB37_10525"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZO22923.1"
                     /translation="MSEGERIRVAILFGGQSAEHEISILSARHVLAALDGSRFEPVLI
                     GIDRTGRWLAQDTRKLLASGHDPRQVRIEEGTPVPMSSALRAAGVAGSGSRIDVVFPV
                     LHGTLGEDGAMQGLLEVAGIPYVGAGILGSAIGMDKDVMKRLLRDAGIPVAEFRTLRR
                     DSFDADPRGVCEELGVLGFPLFTKPANAGSSVGIRKVASATALPAAIRYAFEFDSKVL
                     VEAAVSGREIELAVLGGSPPAVSVAGEIIVEHPDGFYSYEAKYIDEHGARLELPARLS
                     AGELSRAQALALQTFEVLECEGMARVDLFLTRDGRLLVNEINTIPGFTAISMYPKLWA
                     LSGIQPTELVGRLIELALARGRRRAALRRSAFADR"
     gene            complement(7374..8069)
                     /locus_tag="VFB37_10530"
     CDS             complement(7374..8069)
                     /locus_tag="VFB37_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22924.1"
                     /translation="MKTLRVSRRFCGPAVSANGGYFAGLVAGLVSHTLAVRLRVPPPL
                     ETELEVLEREGGIVEVRHGAELIGEGRPSTLTLEVPACPGYLLALGASRNYRGFAHHR
                     FPACFVCGTQRERGDGLRIFAGAVPGRELVAAPWVPDNSLDRGDGKVRPEFMSAALDC
                     PGYYAVTPDDRSMLLGQFTTHLDRLVHIGESCTVIGWRLAAEGRKHEAGTALFDGDGE
                     LCGRAQALWIEPR"
     gene            complement(8092..>9259)
                     /locus_tag="VFB37_10535"
     CDS             complement(8092..>9259)
                     /locus_tag="VFB37_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZO22925.1"
                     /translation="IDTFFPSFHTIAAHFHLSQWAVQQTLTVYMLPLALMSLVQGPLS
                     DAMGRRPVILAGLATYTVASIGCTLAPDFGALLVFRALQGTSAGVGVIVGRAIIRDLF
                     EGPQAQKLLSLVTMIFAFAPAVAPVIGGWLQVLLGWRGVFGFMVAIGTALALSAFVLL
                     PETHPRELRPRLHVGELATTAWGIVRHGEFLLLATAMGANFAALMSFIGAAPAVVEDH
                     WHLGETQFAHLFIPVIGGLLLGGWVSGRLAGRVTYAAQSRLGFILALSGSGFMTILQA
                     LLGAPPILLQQMALCTIALGVQIASPTLSLRMLDLFPRARGSAASVQSCVSIAISALV
                     FGLLSPLLSGSMLTLSEGSFSAALCAFALWNLSQFLLARPPRRDMKPSTGEASP"
BASE COUNT         1625 a         3002 c         3033 g         1599 t
ORIGIN      
        1 ccagctcgta ctccatccgg tcgaatgtca tgccggtcag ccggccattc tccagcacaa
       61 aagccttcgg agagcggttg acaatgatct cgacgctctc ttcctctgcg tcctcgagct
      121 cccaggccga ggccttgaag aatccgcgcg gcttgcgggc catcaccttg acgctccggg
      181 cgcccagtcg gaggctcgtg cggcagcaat ccatggccgt gttgccgaca ccgatgatga
      241 ggacacgctc gccgatcctg tcgatgtgct ggaacgcaac cgactccagc cacgcgatcc
      301 cgatatggat gtgcgccgcg ccctcggccc gcccgggaag cttcagctcc ttgcccttgg
      361 gggcacccga gccgacgaac acggcatcga agccgccggt cgcgagcagc tcgcgcatgc
      421 tggagatggg atgattgagc ttcaggtcca cccccatgtc cgtgatcatg gcgatctcct
      481 cgttgagcac ggtatgaggg aggcggaacg agggaatgtt ggtccgcatc agtccgccgg
      541 gttcggcaaa ctgctcgaag atcgtgacct ggtagccgag cggcatgagg tcattcgcca
      601 cggtcaggga ggcacaaccg gcgccaatgc aggcgatccg ccggccattc ttggccttgg
      661 ggatcttcgg tagcagcccg gccacatcct cgcgtagatc ggcggcgaca cgcttgaggc
      721 gacagatggc caccggcttg ttctcgacgc gtgcccggcg gcaggcaggc tcgcagggcc
      781 ggtcacagac ccgccccaaa atcccgggaa agacattgga gtctcgattc accatatagg
      841 cgtcggtgaa tcggccctga gcgatcaggc gaatgtactc ggggacatcg gtgtgggccg
      901 gacaggccca ctggcaatcc acgactcgat ggtaatagtc gggatgcgag gtatcggtcg
      961 gttccattca gtgccgcagc cggcgagcag cctccaatca gagagaggat ttggagtata
     1021 gaggcgtgcg gccgaatgtc accgtgggat tggccatttg tcgaatgaag cgcagacttg
     1081 acatcagggc gggcttctgc atgcgcgacc gtggccgttg cgccgcggct atactgttca
     1141 cgccatgggc gaccccatcg ccaccgagat ccgggtccgc agccgctcgc ggctgctgga
     1201 agtgagcttc caggatggca gccgcttcga gctgccgttc gagttcctgc gggtccactc
     1261 cccttccgct gaggtgaagg gccacggacc ggggcaggag acgctcgttc tggggaagga
     1321 gcaggtcggg atccgggctg tggagcccgt gggccagtac gccgtgaagc tcgtgttcga
     1381 cgacggtcac gacacggggc tttacacgtg gacctacctt cacgagctcg gcagcaacca
     1441 ggccgtgctc tggcagcgct atctgcagag gcgcgcccaa gccaccggga gctgagatgc
     1501 ggccaccgga ccccctcgcc ccgcttagaa tggcttcccg gcccttccgg caatcccatg
     1561 gcagacccgt tcactgattt tggcttcgag cgcattccct ggcgggagaa agcacgccgg
     1621 gtccgcgggg tcttcgactc ggtgacgagc cggtatgacc tgatgaacga tctcatgtcc
     1681 ggtggcatcc atcggctgtg gaagaggttc accctcacgc tggccaacct gcgcgagggg
     1741 cagtcagcac tggatctggc tggcggcacg ggggatctgg cggccggact cgcacagcag
     1801 gtcgggagtc gcggaatcgt cgtcctcagc gatatcaatg ccagcatgct cgcccgggga
     1861 cgcgaccgcc tcatcgacgc aggacggtcc ggaaatcttc actatgttca ggcgaatgcc
     1921 gaacggctgc ctttcgcatc gcacagtttc gactgcgtca ccatcggctt cggattacgc
     1981 aacgtcacgg acaagaatgc cgccctcgcc gccatgcagc gtgtcttgaa gccaggcggg
     2041 cagcttctgg tgctggagtt ctcccacccg gtaatgccgg ggctgaagcc gctctccgac
     2101 gcgtactgca tgcgcgtgct gccgatgctg ggcaagctgg tcgtcgggga cgagtcgagc
     2161 taccgctatc tcgcagagtc gatccgcatg catccgccgc aggagacgct gctgcagatg
     2221 atgcgggatg ccggcctcga gggctgccgc taccacaacc tttccggcgg gatcgtggcg
     2281 gtccaccgcg gatacaagag ctgaagatgc agcggatgct cgcggagacg cttcagaact
     2341 tgctcaacag aggcctaccc cgctctccaa gagcgcagca gctgtgtgcc gagctcgccg
     2401 gccgcagcgt ggcggtcgag ctgcgggata ttttcagtgt tcgggtggag tcaacgggcg
     2461 caacattgag cgtcacacgc ccggcggggc cggccgatgc gcgtatctgc ggtgggcccc
     2521 tcggtctgct cgcgttggcc ggcgactcac cgcaggccgt cttgcagcgg ggtcaggcgc
     2581 ggatcgaagg cgacagcgag gtcgccggga aattccgcga gctcggacag ctgctgcggc
     2641 cggatctgga ggaggagctc gcactgctgc tgggcgacat tcccgcccac cagatcgggc
     2701 gcttcgcgcg ggcagcgctg aactggagcc ggaacgccgc gtcgaccatg gccgacaact
     2761 tcgccgagta cctcgcgcac gagcagggcg atctcgtgtc ccgcggtgag gccaatcaat
     2821 ttctgcgcgg cattgatgca ctgcgtgagg acgtagaccg gctggccgcg cgaatcgacc
     2881 tattgcagca gcggccccgc acccgctccg cagaacagca cgactcgtga cttccgtcgg
     2941 gatcgctggg ggtatcggcg tgccgcgcat gcggctgcgc gtgcttgcca gattgatcca
     3001 gattcaccgt gtgctgctca agcacggcct ggatgacctg gtgcgggcca cacacctgta
     3061 ccgcccgctg cggttcctct tctatttttc gcccggaatc tggttccagc gctcgcgccg
     3121 cgccgagcgc ggcgagcggc tgaggctcgc cctccaggag ctcggcccga tcttcgtcaa
     3181 gttcggacag gcggtgtcga cgcgccgcga cctgctgccg acggacatcg cagatgagct
     3241 cgcgcgattg caggaccgcg tcccaccgtt tcccggcaac gtggcgcgcc agatcatcga
     3301 gcgcgcccat ggcagaccgg tcaccgaaac cttctcggaa ttcgacgagg agccactcgc
     3361 ggcagcctcc atcgcgcagg tccacgcagc gaagctggcc gacggcaccg aagtcgtcgt
     3421 caaggtactg cgccccggca tgcgcgagat gatcgagcag gatctcgagg ttctgtattc
     3481 gctcgcggca ctggcgcacc gctactgggc ggaaagccgc cggctgcgcc ctctcgaggt
     3541 cgtctcggag tatgaaaaga cgatcctcga cgagctcgac ctgatgcgcg aggctgccaa
     3601 cgcctcgcag ctgaagcgca attttctcgg ctccgatctg ctgtacgtcc ccgagatcta
     3661 ctgggactat tgtcgcgtca acgtgatggt catggagcgc atccgcggca tccagatcag
     3721 cgatcgcgag cggctgtacg cggcgggaac cgacatcgcc cagctggcgg aaaacggggt
     3781 gaagattttc ttcacgcaag tcttccgcca caacttcttt catgccgaca tgcatccggg
     3841 caacatcttc gtgatcgtcg acgaaccgcg cagaccgcgc tacgcagcgg tggacttcgg
     3901 catcgtcggc acgctggatc cgcgcgatca gcactacctc gccgagaatt ttctcgcggt
     3961 cttcgaccgc gactaccagc gtgtcgcgcg tctgcacctg gagtcaggct gggtgccacc
     4021 cgattcgagg ctcgatgaga tggaatccgc ggtgcgtacg gtatgcgagc ccatcttcga
     4081 ccggccgctc caggatatct ccttcggaca gatcctgttg cggctgttcg agatctcgcg
     4141 gcgcttcaac atgcaggtgc agcctcagct catcctgctg cagaagacgc tgctgaatgt
     4201 ggaaggtctc ggtcgcgacc tgtatccgca gctggatatc tggaagacgg cgagcccgat
     4261 cctgcgccag tggatgcgtg agcgcatgag cgtcggtcag ttgctgaaga acttgcgcag
     4321 agatctgccg gagctgatgg agaccgcgcg cgtcctgcca tcgctcgtga aggcattcgc
     4381 gcatcgcgcg caaggaggcg tgctgcgcat ggaagttgaa tcgcaggcgc tcgacgctct
     4441 gcacacccgg atccgcaatg tcgggcggcg acgcgacgcc atcaccatcg gtgcggccat
     4501 tctcctcggc ggcctcgtct ggcttgcact gagccgtggc ggtcactggc cgggatggat
     4561 cctgacgctg gccgggaccg tgtggctgct ggtagcctca cgcctgccgt gaccgttcgc
     4621 cgcgactcac cggcgcgcga ctctagcgca cctgctccgt gccatcgccc atgcaaaacc
     4681 cgcgctccct gaccgagggc tcgatcagtc gagcgctgct gcagttctcg ctgccgatcc
     4741 tgtatgcgaa tgtcctgcaa tcgctgaacg gttcggtcaa ctcgatctgg gtcggccgct
     4801 ttctcggcga agcggccctc acggccacct cgaacgcgaa cacggtcatg ttcctgctga
     4861 tcggtgcggc gttcggtatc gccctcgccg cgacgattct catcggacag accatcgggg
     4921 ccaacgacat cgcgcatgcc aagcgcgtcg tgggcacgag cgcaacattc ttcgcggcca
     4981 tctcgatcgc gatggcagtc gccgggctga tgctatgccg acccctgctg attgcgatga
     5041 agacgccggg agactccctg cagctcgcgg tggattacat gcgggtgatc ttcctcgccc
     5101 tgccttgcct gtacatgtat gccttcgtga tgtcggtgct gcgtggcgcc ggggattcca
     5161 agacgccgtt ctatttcatg ctgctgtcgg tggctatcga catcgccctc aacccggtct
     5221 tcatattcgg gctggggccg gcgccccggc tcggcatcgc cggctcggcg ctcgcgacgt
     5281 tcatggcgca ggcgatcagc ttgaccgccc tcatcgtgca cctctacaga cgccggcacc
     5341 tgctcgtgct gcacaagtca gaagcagcgc tgctacggat cgattgggtg atcgttggtg
     5401 cgctcgtccg caaaggcatc ccgatgagtg ctcagatgct cgtactctcg ctcagcggcg
     5461 tgttgatgat cacactcgtc aatcgtttcg gtgtggacac caccgcggcc tttggtgcgg
     5521 cgctgcagct gtggaactac atccagatgc ccgccttcgc cgtcggcatg ggggtctcgg
     5581 ccatggcggc gcagaatgtc ggggcgaaga agtgggaccg tgtcaactcg atagcccgtg
     5641 tcggtgtgct ctacagcgtg ctgctgaccg gctccgtcat tcttgtgctc gaggttctca
     5701 atcgacatgc tttctcgctc tttctggtag aagactcaag ggccatgctc gccgcgagcc
     5761 acctgaatcg catcgccaca cccgggttcg tgttcttcgg catttccatg gtcctgttcg
     5821 gcgtggtgcg cgcaaccggt gcggtcatgg cgccgctgat ctgtttgacc gtctcgctgc
     5881 tgctggtacg cgtgcccctc gcggacctgc tgctgggccg gttgcacgtg gatgcgatct
     5941 ggtggagctt tccgatctcg tcggctctcg ccgccgtgct cgcggtcctc tactacaaat
     6001 tcggcggctg gcgctcggcc accatggagc ggccgcccaa atccgagccc gtgcacccgg
     6061 tggaagagac ctgaggcggg tgagacctga cgcgcggaac cgcggtcgca gcctgagtag
     6121 gctgggcagg taacaaagag gagcgggacg tgagcgaagg tgagcggatt cgtgtggcca
     6181 tcctgttcgg cgggcaatcc gccgaacatg agatctcgat cctgtcggcg cggcacgtgc
     6241 tcgccgcgct cgacggctcg cgattcgaac ccgtactcat cggcatcgac aggaccggcc
     6301 gctggcttgc gcaggacacc cggaagcttc tggcgagcgg ccacgatccg cgccaggtca
     6361 ggatcgaaga gggcacaccg gtgccgatga gctcggcgct tagggccgcg ggggtagccg
     6421 gatcgggaag caggatcgac gtcgttttcc cggtgctaca cgggaccttg ggagaggacg
     6481 gcgcgatgca gggtctgctc gaggtcgccg gcattcctta tgtcggggcg ggcatactgg
     6541 gttcggcgat cgggatggac aaggatgtca tgaagcgttt attgcgtgac gccggcattc
     6601 cagtggccga gttccgcacg ctgcgtcggg attcattcga tgcggacccc aggggcgtct
     6661 gcgaggaact cggtgtcctg ggcttcccgc ttttcaccaa acccgccaac gctggctcat
     6721 cggtcgggat ccgcaaagtc gcgagcgcca ccgcgttgcc ggctgcgatc cgttacgcct
     6781 tcgagttcga ttccaaggtt ctggtggaag ccgcggtcag tgggcgcgag atcgagctgg
     6841 cagtgctcgg agggagcccg cccgccgtat cggtggccgg cgagatcatc gtcgagcacc
     6901 cggacgggtt ctactcatac gaggcgaagt acatcgacga gcatggagct cgcctcgagc
     6961 tgccggcccg gctcagcgcc ggggagctga gtcgcgccca ggcgctcgcc ctgcagacct
     7021 tcgaagtgct cgagtgcgaa ggcatggcgc gcgtggatct ttttctgacg cgcgacggcc
     7081 ggctgctcgt caacgagatc aacacgatcc cgggattcac ggcgatctcg atgtatccca
     7141 agctgtgggc gctgtcgggc attcaaccga ccgagctggt cggccgtctg atcgagctgg
     7201 cgctcgcccg tggcaggcgc agggctgcac tgcgccgatc ggccttcgcc gaccgctagc
     7261 gctcaagtac atgggcgagg tgcatcgcgc cgagcgggcg cacagggagg tgcgcccagg
     7321 gattgcgcgc agcaggacag cgcagcgcaa tggcgcgatc ccgagcaatc agactagcgc
     7381 ggctcgatcc acagcgcctg ggcacgcccg caaagctcac cgtcgccgtc gaacaacgcc
     7441 gtccccgctt cgtgcttgcg gccttccgcg gcgaggcgcc agccgatcac cgtgcaggac
     7501 tctccgatgt gcacgagccg atcgagatgg gtggtgaact ggccgagcaa catggagcga
     7561 tcatcgggag tcaccgcgta gtagccggga caatcgagcg ccgcggacat gaattccgga
     7621 cggaccttgc catcgccgcg gtcgagggag ttgtccggca cccagggtgc agccaccagc
     7681 tcgcggcccg gaaccgcacc tgcgaagatc cgcaatccat cgccccgctc gcgttgcgtc
     7741 ccgcagacaa agcacgccgg aaagcggtga tgggcaaatc cacgatagtt gcgcgaggcg
     7801 ccgagcgcga gcaggtaccc ggggcacgca ggtacctcca atgtcagcgt ggaaggacgg
     7861 ccctccccga tcagctccgc gccatggcgc acctcgacga tgccgccctc ccgctccagg
     7921 acttcgagct cggtttcgag cggcggcgga acccgcagac gtaccgcgag ggtatgactc
     7981 acgagcccgg caaccaatcc cgcaaagtaa ccgccgttgg ccgagacggc gggcccgcag
     8041 aaacggcgac tcacgcgcag cgtcttcatg atgcggccct ctccggcatc ctcatggact
     8101 cgcctcgcca gtggatggct tcatgtcacg tcgcggcggc cttgccagca ggaactgaga
     8161 caggttccag agcgcgaagg cgcagagagc ggcagaaaag gagccctcgg agagcgtgag
     8221 catcgagccg ctcagcagcg gcgagagcaa tccgaacacc agcgcgctga tggcgatcga
     8281 gacgcaggac tgcacggagg cggctgagcc gcgcgcccgc gggaaaagat cgagcatgcg
     8341 tagactgaga gtcggcgagg caatctgcac gccgagcgca atcgtgcaga gggccatttg
     8401 ctgcaacagg atcggtggcg cgccgagcag agcctgcagg atggtcatga agcccgatcc
     8461 gctcagagcc aggataaaac ccagccggga ctgcgccgcg taggtcacgc ggccggccag
     8521 ccgtcccgag acccagcccc ccagcagaag gccgccgatc accgggatga acagatgcgc
     8581 gaactgcgtt tcccccagat gccagtgatc ctccaccacc gcaggcgctg cgccgatgaa
     8641 gctcatcagt gcggcgaagt tagcgcccat cgccgtggcc agcagcagga actcgccgtg
     8701 gcggacaatg ccccacgccg tggttgccag ctcgcccaca tgcagccggg ggcgcaactc
     8761 ccgcgggtga gtttccggca acagaacgaa ggcggacagg gcgagcgccg taccgatcgc
     8821 gaccatgaat ccgaacacgc cccgccatcc gagcaacacc tggagccaac cgccgatgac
     8881 cggcgccacc gccggtgcga acgcgaagat catcgtgacg aggctcagca gcttctgcgc
     8941 ctgcggcccc tcgaaaaggt cgcggatgat cgcacggccg acgatcacac ccactcccgc
     9001 gctcgtgccc tgcagggcac gaaacacgag cagcgcgcca aagtctggcg cgagcgtgca
     9061 gccgatcgag gccaccgtat aggtcgcgag cccggcgagg atcaccggcc ggcgtcccat
     9121 tgcatccgac agcggcccct gcacgagcga catcagcgca agaggcagca tgtaaacggt
     9181 gagcgtctgc tggactgccc actggctgag atggaaatgc gccgcgatgg tatggaagga
     9241 cgggaagaac gtatcgatg
//
LOCUS       DASRHN010000267        30311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2233149, whole genome shotgun sequence.
ACCESSION   DASRHN010000267 DASRHN010000000
VERSION     DASRHN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 30311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30311
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233149"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="flhB"
                     /locus_tag="VFB37_10540"
     CDS             complement(<1..1066)
                     /gene="flhB"
                     /locus_tag="VFB37_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HZO22926.1"
                     /translation="MAEHEPERTEQPTPKRLEEARKQGQVPRSVELSTAAVLLTAGGG
                     LNFLGRHMGGQLDGMLSSALTLSREQSLDETFMIASLSRDMLQALVACAPILGLTLIA
                     ALSAPMLLGGWNLSFSALAPDFNRLNPMEGFGRMFSARGAVELGKALAKFTALALVAV
                     HILHSKSSELLGLGMEPTRVAIGHAASVTGYAFLMLAGTLGLIAAVDVPWQMWQHTRR
                     LRMTRQEVREEMKESEGAPEIKGRIRNLQREVAKRRMMQEVPKADVVVVNPTHFAVAL
                     RYDERRMRAPIVVAKGADLIAARIREVATESLVPIFEAPPLARALFRSVEIGGEIPAS
                     LYNAVAQVLTYIYQLKTARSQ"
     gene            complement(1070..1852)
                     /gene="fliR"
                     /locus_tag="VFB37_10545"
     CDS             complement(1070..1852)
                     /gene="fliR"
                     /locus_tag="VFB37_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZO22927.1"
                     /translation="MITLTTGQLETWLAQVLWPFVRIGACFMVAPAFGAVFVPARIRI
                     VLAGAVTLAISPLLPPPAAIVPFSAAGLLVTVQQLLIGVALGFSLQLLFDAVTLGGQL
                     LANSMGLSFAFNIDPLRGEEVPALGQFYTILVMLTFMALNGHLVLIEVLVDGFRTLPV
                     GTDGLGQDGLWTLVEWGSQLFVGALSIALPGVTALLIVNVAFGVMSRAAPQLNLFAVG
                     FPISLVFGLVIVLAGMPAVQTTFTHLLGDGFELLRKLTVAGG"
     gene            complement(1887..2156)
                     /gene="fliQ"
                     /locus_tag="VFB37_10550"
     CDS             complement(1887..2156)
                     /gene="fliQ"
                     /locus_tag="VFB37_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HZO22928.1"
                     /translation="MTPETVMTIGSRALEITLMLAAPLLLVALVTGLIVGAFQAATQI
                     NEMTLSFIPKLLAMALAMVIAGPWMLKTLVGYTRELFESIPGLIN"
     gene            complement(2168..2923)
                     /gene="fliP"
                     /locus_tag="VFB37_10555"
     CDS             complement(2168..2923)
                     /gene="fliP"
                     /locus_tag="VFB37_10555"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZO22929.1"
                     /translation="MRAARSITLAGLCGAAILVPSAVMAEPGLPAIAVQSGAGGGQTY
                     TLTLQVLALMTALTLLPAVLLMMTAFTRIIIVLGILRQALGAGQTPPNQVMLGLALFL
                     TFFVMTPVINRVNEEAFRPYSAGTIEATVALDRALVPIKKFMLDQTRESDIATFVRIS
                     GGKGFATAQDVPLTMLVPAFVTSELKTAFLMGFLIFIPFVIIDLVVASVLMSMGMMML
                     SPVLISLPFKLMLFVLVDGWSLVMGTLASSFYS"
     gene            complement(2923..3399)
                     /gene="fliO"
                     /locus_tag="VFB37_10560"
     CDS             complement(2923..3399)
                     /gene="fliO"
                     /locus_tag="VFB37_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZO22930.1"
                     /translation="MRASPSTFLTVLFGAASLCATLCAAAADAPAATAHPFAAPQPAT
                     PIAPAGLGGLGQVTLALLAVLATVFVIAWMARRMRSFGNRVGNALDVLADIPLGQKER
                     AVLLKVGQTQILVGVAPGRVNTLHVLAEPIELSRSAVGMADGRPSFKALMLRSLGK"
     gene            complement(3405..3740)
                     /gene="fliN"
                     /locus_tag="VFB37_10565"
     CDS             complement(3405..3740)
                     /gene="fliN"
                     /locus_tag="VFB37_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZO22931.1"
                     /translation="MNANVAEPRAAEFDNLQDDAPQSGRDVNLEVILDVPVTLSMEVG
                     RTRIPIRNLLQLNQGSVVELDRAAGEPLDVFVNGTLVAHGEVVVVNEKFGIRLTDVIS
                     PAERIRKLK"
     gene            complement(3751..4767)
                     /gene="fliM"
                     /locus_tag="VFB37_10570"
     CDS             complement(3751..4767)
                     /gene="fliM"
                     /locus_tag="VFB37_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HZO22932.1"
                     /translation="MSTDKVLDQAEIDALIHGVDSGKVDTNGAPQPGGDVRSYDFGSE
                     LRIVRGRMPTLEMINDRFARLFRVSLYNLLRRSPEIAVAPIQVKKFAEYAHTLHVPTS
                     LNMVKMNPFRGTALIVLDPKLVFAVVDNFFGGSGRHAKIEGREFTATEQRIIHMMLRS
                     VFADLHEAWAHIAPVEVEFVQAEINPHFANIVSPSEIVVITSFHIELDGGGGNLNVTM
                     PYSMLEPLREILDAGVASDRAEKDERWASSLREDLEDAEVQINSRLGRTSITLAQLLN
                     LKPGDVLPCDFSGKVTLLAEDVPVFRGSFGLSRGLQAVKVDERIRRSAPPAPATSIAI
                     AKRT"
     gene            complement(4780..5289)
                     /locus_tag="VFB37_10575"
     CDS             complement(4780..5289)
                     /locus_tag="VFB37_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HZO22933.1"
                     /translation="MADVPVLAPAAEDAPGKKKSKWLIIGLVAAVVLVGGGVGAFFLW
                     PQADAHSKQAQEKAKEPLTAPLYVPLDPPFVVNFEGDPLVRFLQVAAQVMTRDPETVA
                     LLKANDPAVRNDLLLLLGNQKSAELGTRDGKEHLRAEALAAIRHVIETAGGKPDKVEA
                     IYFTSFVMQ"
     gene            complement(5501..6469)
                     /locus_tag="VFB37_10580"
     CDS             complement(5501..6469)
                     /locus_tag="VFB37_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZO22934.1"
                     /translation="MLNFVQTLARSINAGQTADAPAAASPSAADVSADDGGSDADAKG
                     AGAGQGDLALALVSQSIAAALGLPAVMPPMAAPSPTGQSTPATSTASGTEAIDGAGAQ
                     PASANGSAVQTLVTLIAEDAAAQSKDKTPATGQADATSAPLVNNTATGSADSAPATAA
                     PTIPHLGIASHFSVQHSSPDSNSVGGGLRAQVGTPAWSEELGGQITWMAHQGIESASL
                     QLSPEHLGPLEVHISVHDGAASVWFGATQPDTRAALEQALPRLREMFATQGLTLMDAG
                     VSHESPRNQSKPPRATAPAAVSAVAATESTTTSASGLSLGLVDTYA"
     gene            complement(6885..7340)
                     /gene="fliJ"
                     /locus_tag="VFB37_10585"
     CDS             complement(6885..7340)
                     /gene="fliJ"
                     /locus_tag="VFB37_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HZO22935.1"
                     /translation="MKRAQRLQIVQRVVDDQERSRAEALAGSERRLVESETKLRELES
                     YHAGYQRDFALRAESGMDGVLARDYQAFLCRLDEALRQQNQVVIRARAQRDGELRTWQ
                     GAAQRAEAVGQMVKRFQTEEERALDRREQHEADERSQRAWAHGLALRGA"
     gene            complement(7343..8740)
                     /gene="fliI"
                     /locus_tag="VFB37_10590"
     CDS             complement(7343..8740)
                     /gene="fliI"
                     /locus_tag="VFB37_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HZO22936.1"
                     /translation="MSALDASRAEIWRQGLARRLRRLEQTPPPAIEGSLTRMVGLTLE
                     AVGCQASIGDYCDVITGDGFRVESEVVGFAGDRLYLMPTGDAQGLGPSARVIPRQRAG
                     TVRVGPELLGRIIDGAAHPLDDLGPLECETRVRLTGRPMNPLSRRPITEPLDVGIRSI
                     NSLLTVGRGQRIGLFAGSGVGKSVLLGMMARYTSADVIVVGLIGERGREVQEFVERIL
                     GPKDRKRAVVVATPADHPPLMRLHGAWLATAIAEYFRDRGLNVLLLMDSLTRFAQAQR
                     EIGLAIGEAPATKGYPPSVFARLPQLLERAGNGMQGSGSITAFYTVLTEGDDHNNDPV
                     ADAARAILDGHIVLSRRIAESGQYPAIDIEASVSRAMHEIASPAQIQSARQFRQILAT
                     YQQNRDLIAIGAYQRGSDPRVDNAITLWPRVQKFLQQDMTERVNFAASLTALSAVLAE
                     SEASRGETGKPSDRQ"
     gene            complement(8737..9390)
                     /locus_tag="VFB37_10595"
     CDS             complement(8737..9390)
                     /locus_tag="VFB37_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HZO22937.1"
                     /translation="MSDPQRAPAAVERWSLPRVDGPVIGNERPARKSADGARALQESE
                     ARGYQAGLARAQAEMQAGHAALDTRLKQLDSILNLLAKPLQEMDAAIEKELLQLALTV
                     GKQLARRELRTDPAQVIAIVRESLAQLPAAAREIRVHLHPEDAATVRERLAAPASERA
                     WTLVEDPTLTRGGCLVRTDTSQIDVRLESRISAVIANALGEERAPERPTPASGEPET"
     gene            complement(9383..10396)
                     /gene="fliG"
                     /locus_tag="VFB37_10600"
     CDS             complement(9383..10396)
                     /gene="fliG"
                     /locus_tag="VFB37_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HZO22938.1"
                     /translation="MSDKRESSERSGTERAAILLLTLGEQEAAQVLKHMGAREVQRIG
                     SAMATLANVSREEVQGIMTQFSSQVESETSVGVGTEEFLRKVLVEALGQEKAANIIDR
                     ISIGRSTKGLEALKWMDARAVAELIRLEHPQIVAIVLAYLEPDQSADILSNLPPDMRS
                     DVLVRIATLDGVQPSALTELDDIMEKQFAGKGAHRTSTLGGAKAAANIINNLEPSQES
                     VLMEQITATDEALSARIQDLIFVFDNLLDIDDRSMQELLRQVPSDKLLLAMKGCEDVM
                     KEKIFKNMSQRAAEMLKDDLESKGPVRISEVEAAQKEILQTARKLAEAGTIALAGKGE
                     EYV"
     gene            complement(10389..12071)
                     /gene="fliF"
                     /locus_tag="VFB37_10605"
     CDS             complement(10389..12071)
                     /gene="fliF"
                     /locus_tag="VFB37_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZO22939.1"
                     /translation="MNAEVAQLPAPAGLPAGLRPLLLLVGIAMAVAAGVTVVLWSRGP
                     TYSLLYANLGTEDQAQITQALESAQIPYRIQPGSNSIEVPAERLSDARLKLAGQGLPE
                     GGGGFATMEKDPGFGVSQFMENARYQHALETELARTISALRPVEGARVHLAVPRQSAF
                     VRDHKGASASVFLQLKQGRRLEQEQIQAIVNLVASSIPDLEANQVTVVDQQGRLLSSP
                     DGHDQFGLRDQQYDMIHRLEDDYAQRIEALLSPVVGSGRVRAQVVAQMDMSVTEEARE
                     QYKPDSQIVRSEQSSEQTSRDGSGAQGVPGALSNQPPASGVALPPGSGSTPPAAAPKP
                     PAAATAQSGAAAAAAATSPIAGSGAGGTDSTSKSETKNFEIDRTLDYTRQPAGRLRRL
                     TVAVLVDNLRTVGKDGKATETPLSKDQLDHLTQLVKDAVGFDEARGDNVNVVNASFTQ
                     EAPPSNDGELEKPKFWESPLFRDLAKILAGLLVLLVLVLSVLRPLVKSLMGPARPALQ
                     RAPEPAPAPALVGGRSPTVVAHEQQIAQARNLVSQDPKRVAQVVRGWVATDE"
     gene            complement(12091..12417)
                     /gene="fliE"
                     /locus_tag="VFB37_10610"
     CDS             complement(12091..12417)
                     /gene="fliE"
                     /locus_tag="VFB37_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZO22940.1"
                     /translation="MSQMEIDRVLAQIRSLSNQTRVGAAPTNPTAQAGPSEFANILSK
                     GIDQVNQSELRATQLADAFQRGAPGVELPQVMLEMQKASVSFRALTEVRNRLVSAYQD
                     IMNMQI"
     gene            complement(12441..13493)
                     /locus_tag="VFB37_10615"
     CDS             complement(12441..13493)
                     /locus_tag="VFB37_10615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZO22941.1"
                     /translation="MAEDPIDQQTAGACVARIEPEPVACATSSTRLLELARRVAASDC
                     TVLIVGESGTGKEVLARYIHRRSPRSAQPFVAVNCAAIPENMLEAMLFGYERGAFTGA
                     HAAHPGKFEQAQGGTLLLDEVTEMPLGLQAKLLRILQEREVERLGGRTPIALDVRVLA
                     TTNRRLREDVAAGRFREDLFYRLNVFPLAIAPLRMRQDDVLPLAMQLLGSRCRPGALI
                     PALSAQAAHLLLTYSWPGNVRELDNLLQRALILVNGPVIRPEHIQFELANEGAVTQAP
                     AQVNPTLDSAPAVAPQSGSLASSLGQTERDLILDALRSGQGNRREVAERLGISQRTLR
                     YKLARLREAGIDVPAA"
     gene            complement(13673..14920)
                     /locus_tag="VFB37_10620"
     CDS             complement(13673..14920)
                     /locus_tag="VFB37_10620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZO22942.1"
                     /translation="MSAAESVLIPELLRADAAQSDADRPMVPELVAFEDHRIRIPTGS
                     SVVIRDVIALIRQVAGHDSTVLVLGESGTGKEVAARAIHDLSPRRSRPFVAVNCGAIP
                     AELLESELFGHERGAFTGAVSARKGRFEIAEGGSLFLDEIGDMSPTMQVKLLRVLQER
                     VFERVGNHVPVRCNVRIIAATHRNLEESIARGSFREDLFHRLNVFPIEMPPLRARIED
                     LPMLVRDCITSNVAEGRGRVQLSPRALGALALCPWTGNVRELANLIERLSIVCAGRVV
                     EVRDLPPKYRPPEDWKLQGTEFDSSLCVSSLDDPSQSIAIEEAIVSEETALSVLESAE
                     LVPAECLSSAGAAVADAQAVLPDEGLDLRVHLLAIERALIEQALQRSRGTVAHAARLL
                     NLRRTTLVEKMRKLGMASSAEDA"
     gene            complement(15070..15636)
                     /locus_tag="VFB37_10625"
     CDS             complement(15070..15636)
                     /locus_tag="VFB37_10625"
                     /inference="COORDINATES: protein motif:HMM:NF028133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZO22943.1"
                     /translation="MYDGSDTVVLYDELAFQDVLPVLWRPLNTPVDRDLAASFDERNI
                     RLLQAWDAMEEHGPVEKPDDSPHAAELLRLDLKVNLLLDLVGQILANSRPRPAPSPVR
                     FNALGAVWRASGPLPEAGEQGIAEIYLRDCLAEPLRLAGRVTNVTPDGHIKVKFLPLG
                     EGVADLIEKLAFRRHRRQIAGARQPRRG"
     gene            complement(15629..16012)
                     /locus_tag="VFB37_10630"
     CDS             complement(15629..16012)
                     /locus_tag="VFB37_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZO22944.1"
                     /translation="MPAAAPSRTCAFDSSVARLGPAAAHSNGFAPMLMESALEHIAQA
                     RRQLHSGEGSERRELLHGAVQLLETLRNSLDLHRGDAYAVNLDDLCDYLSRQLAAASL
                     HDRVATLDEVSHLLREIRCAWPMYV"
     gene            complement(16154..16864)
                     /locus_tag="VFB37_10635"
     CDS             complement(16154..16864)
                     /locus_tag="VFB37_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22945.1"
                     /translation="MTVHIDSSSQLIHDQEQNLAVTCPHCEVVSHVTPSAVPRFEDLQ
                     AHRPKQIGVVYRCDACGAPIFTRFMVRMYGPSRVELAPQFTEVERAREKFSFTYLPQD
                     VEILFREGLACFSAGAFNAFASMCRRTAQAVFADLGEAGKLRLFDELNTARDLAGLTS
                     ETYGKLKTILFGSESDPRPNPPLLDSYEAGIVLEVMKDVLYEAYVRKGKLRQAILVRR
                     FFLDETAANLAPVTRASG"
     gene            complement(17042..19600)
                     /gene="mprF"
                     /locus_tag="VFB37_10640"
     CDS             complement(17042..19600)
                     /gene="mprF"
                     /locus_tag="VFB37_10640"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HZO22946.1"
                     /translation="MSKDFSALRWIGPLTALLVFGLVVWVLHNELARLHVHSVFVHLH
                     RIPRRNVLIALGFTAASYWLLSTYDALALMYRRRVLPYRRILFTSFIAYSFGHTLGFA
                     AFTGAAIRFRLYSTAGVTAVDVATISAFCSLSIGIGLATLAGISLFITPEQSASVLHL
                     QQGWSMAVGVVLLALVAAYGLWASTARRELEIRGWALRPPGAAIGLTQIVLSVIDLSL
                     SSAVLWWLLPAEAHVSFIAFVGAYAAAVIVGIISHVPGGIGVFETIILYALPHVPRDA
                     LLGSLLAYRGVYYLVPLLVGTLLFGTEELLEQRGRLARARDIAAMYITPVVPQIAGAL
                     TFLAGAILLLSGTTPAIDTRLQVLYRILPLLVVEVSHLAGSVIGLGLLILSRALFRRV
                     RTAYHIAVWLLLAGIIASLLKGLDFEEATILALVLGVLTLGRNAFHRPTAILSERVTP
                     VWIVSIVGVIALAVWVGFIAYRRVEYSDELWWTFALDGNAPRMLRASLVVVVLLAAYL
                     LQNLLRPAQPPPAVAGPQDLERARAVIACCDLTVANAALTGDKRLLFNDAGDSFVMYQ
                     IAGRSWIALGDPVGPEAGAEELVWRFREMSDHHGGQTVFYQASGARLPLYVDLGLAAL
                     KIGEEARVPLTEFSLEGAARADLRQAHRRAVRDGATFEVVRPDEVAPLLPDLQKISDS
                     WLASKSTGEKRFSVGAFSAGYLRNFPMAVVRCDGNPTAFANLWVSGTKEELSVDLMRF
                     GNDAPRGAMDYLFIELMKWGRQEGYGWFNLGMAPLAGLETHPLAPAWHRVGNFVFRYG
                     EHFYNFEGLRRYKGKFEPVWVPKYLVARGGLALPRVLVDVSMLIAGGMRELFAR"
     gene            complement(19644..21317)
                     /gene="ettA"
                     /locus_tag="VFB37_10645"
     CDS             complement(19644..21317)
                     /gene="ettA"
                     /locus_tag="VFB37_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZO22947.1"
                     /translation="MAQYIYTMNRVSKVVPPKRVILRDISLSFFPGAKIGVLGLNGAG
                     KSTLLKIMAGLDTGIDGEARPQSGIRVGFLPQEPQLDPQKTVRTTAMEGVGEAFRNIE
                     RFNEISDRFAEPLPDEEMNALLEEQARLQDAIDAAGGWELERKLEIAADALRLPPWDA
                     SIATLSGGEKRRVALCRLLLSAPDMLLLDEPTNHLDAQSIAWLERYLEEYPSTVIAVT
                     HDRYFLDNVAEWILELDRGHGIPWHGNYSSWLEQKQQRLALEEREREALRKTLERELE
                     WVRQNPKARQAKSKARLQRYEELASREFQERNETNEIYIPPGERLGELVIEAAGLRKA
                     YGERLLFDNLSFNLPRGGIVGIIGPNGAGKTTLFRILTGAEKADAGEIRMGPSTRLAY
                     VDQSRQSLDDSKTVWQEISGGLDMLKVGNYETPSRGYVGRFNFKGSQQQQPIGDLSGG
                     ERNRVHLAKVLRSGGNVLLLDEPTNDLDIETLRALEEALVNFPGCAVVISHDRWFLDR
                     IATHILAFEGDSQAVWFEGNYQEYMEDYRRRKGDEAVQPHRIKYKPLTR"
     gene            21467..22450
                     /locus_tag="VFB37_10650"
     CDS             21467..22450
                     /locus_tag="VFB37_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22948.1"
                     /translation="MNADHQDARFPGGPRSSLGVVLSERLARYAFGNGHPFGPDRHAA
                     FLREFESRGLARRVQIIEPRDATAEELLQFHTPGYVDLVRTRSADGHGFLDYGDTPAF
                     RGMYEAATVVVGSTLNAAEAIMSGQCRRAFVPIAGLHHAARDRAAGFCVFNDCGVLIE
                     RLKRQCSLERIAYVDIDAHHGDGVFYAFEDDPAVIIADTHEDGRYLYPGTGLASETGR
                     GEAVGTKLNLPLQPGADDGVFSRLWPHMLAHLERFQPQFIILQCGADSLEGDPITHLR
                     FSPKTHGRAARELAALADRLGHGRLLALGGGGYNRTNLARAWTAVVESLLS"
     gene            22540..23796
                     /gene="glyA"
                     /locus_tag="VFB37_10655"
     CDS             22540..23796
                     /gene="glyA"
                     /locus_tag="VFB37_10655"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZO22949.1"
                     /translation="MFRKSPDLAAFDPELARAIQGERQRQEDHVELIASENYTSPRVL
                     EAQGSVLTNKYAEGYPGRRYYGGCEYVDIAEQLAIDRAKKLFGAAYANVQPHSGSQAN
                     AAAYYALVQPGDPILGMSLDHGGHLTHGAKVNFSGKYFKATQYGIRPDTGEIDYEQVQ
                     RLAEEHRPRMIIAGFSAYSRIVDWARFAQIAKSVGAYFVVDMAHVAGLVAAGLYPNPI
                     EHADVVTTTTHKTLRGPRGGLILARPNDEIHKKLNSLVFPGTQGGPLMHVIAAKAVAL
                     LEALQPDFNEYQRQVVANARAMCARLIKKGFPIVSGGTDNHLFLMSLVDRKITGKDAD
                     AALGRANITVNKNAVPNDPRPPMIASGLRIGTPASTTRGFKEREIEQVADFIAEVLDA
                     EGSEAAVRRVQPQVLDLCRRFPVYGS"
     gene            23815..24387
                     /gene="nrdR"
                     /locus_tag="VFB37_10660"
     CDS             23815..24387
                     /gene="nrdR"
                     /locus_tag="VFB37_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZO22950.1"
                     /translation="MHCPFCGHVETKVNDSRLAGEGRQIRRRRECLSCGERFTTFETA
                     ELLMPVVVKGNDVREAYDEAKLRIGMEKALEKRPVSREAIDEALSRITHRVRILGDRE
                     VPSRIIGEYVMDELRQLDEVAYVRFASVYRHFQDVEAFHEEIKRLRSQRAAPAQHPTR
                     GSKRGSAKEASKDQLPLLPADPPANGSKQA"
     gene            24384..25475
                     /gene="ribD"
                     /locus_tag="VFB37_10665"
     CDS             24384..25475
                     /gene="ribD"
                     /locus_tag="VFB37_10665"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZO22951.1"
                     /translation="MITDFDRYAMNRALALAERGLETTDPNPRVGCVIAKNGRIIGEG
                     WHQRAGEAHAETAALRAASEAVSGSTVYVTLEPCSHHGRTPPCAGALIEARIARVVYA
                     VQDPDPRVSGEGAAALTRAGVMVERGLMEAEATELNIGFMKRMRHGLPWVRLKLAMSL
                     DGRTALADGSSQWITGGPSREDVHRWRARSSALMTGVGTILADDPQLNVRLPGSNHRQ
                     PLRVILDTRLRTPPDARLFSVEGEVLILTATTVAEAAEALAAGGASVEIIPAFAGLLD
                     LSAVLARLGELEINEVLVEAGPTLSGQLLTGMFVDELLLYVAPKLLGPQARPLINLPE
                     VQSLQEARGFSLFDVKRTGEDLCLRLRPH"
     gene            25493..26110
                     /locus_tag="VFB37_10670"
     CDS             25493..26110
                     /locus_tag="VFB37_10670"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZO22952.1"
                     /translation="MFTGIVQDVGRVKSLERRGGDVRITIELGKLDIGLFHVGDSICV
                     QGCCLTAVELHGKSFEADVSTESLRLTTLGSLEPGSSVNLEASLRAGDPLGGHLVSGH
                     VDGIAKVVSISGDARSQRIRFSVPRELARYIARKGSVALDGVSLTVNEVEGEEFGVNI
                     IPHTQQITTLGRLTTGARVNLEVDQVARYMERLAEYSADFRGTLR"
     gene            complement(26297..26476)
                     /locus_tag="VFB37_10675"
     CDS             complement(26297..26476)
                     /locus_tag="VFB37_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22953.1"
                     /translation="MSIALDAASPEPVMGAPRLVLQEGRGRPAPAVSEHPDLALGMAG
                     FQVAVSEKDPEEGPQ"
     gene            complement(26629..27159)
                     /locus_tag="VFB37_10680"
     CDS             complement(26629..27159)
                     /locus_tag="VFB37_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO22954.1"
                     /translation="MSVVTCAIESARLRLRQFVKADAAFILELLTDPEFLRHIGDRGV
                     HDIADAQRYIRESPGASYSRYGFGLYALERKDGGPVLGMCGLLRRDTHPDVELGFALL
                     PAARGHGYAREAAAATLRYAVQELQLTRLVALTAPDNTASIRILNELGFRFERMAKLS
                     PSGPSRVFVFEAAKPS"
     gene            complement(27174..27905)
                     /locus_tag="VFB37_10685"
     CDS             complement(27174..27905)
                     /locus_tag="VFB37_10685"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZO22955.1"
                     /translation="MSSSTWTAHAVASEAAACTLDLWRAVEAQHQVATRALVDSLEEQ
                     EILESVLEQAKPPIPADCRRYDYLLYTPYRYPPPPPNGSRFRAFGDPGVWYGAEALRT
                     SCAEVGYWRWRFVADSHGLSRLDGVPHTIFQASACGPAVDLRAKPFSRDAKKWHNPNQ
                     YGDCQGFARVAREAGTRILRYESVRDPEHGGCAAVLDCRAFASTGGVRKRQTWFLTID
                     AERASWIRAGGRQAESHEFVFKTGE"
     gene            complement(27865..28239)
                     /locus_tag="VFB37_10690"
     CDS             complement(27865..28239)
                     /locus_tag="VFB37_10690"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZO22956.1"
                     /translation="MILRPANAQDPAAVLSKAAVRASEELGLRQTQLARLLGLSPATT
                     SRLAAGTWLLPQDSKAWELAAAFVRLYRSLAAITGGKSQAMRDWLHSRNDALGGEPAE
                     RILSAEGLIHVLQYLDGARGRI"
     gene            complement(28316..28492)
                     /locus_tag="VFB37_10695"
     CDS             complement(28316..28492)
                     /locus_tag="VFB37_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22957.1"
                     /translation="MPRSAATSASRTEVRNALAFVRDQGVVLVSAKGPVPRLVDFMLG
                     PTLLAAAAVGAVRG"
     gene            complement(28607..28822)
                     /locus_tag="VFB37_10700"
     CDS             complement(28607..28822)
                     /locus_tag="VFB37_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22958.1"
                     /translation="MPRIAAWSVTPGQTSMEILPPADTMGMKPTGIKARSESAGSTSS
                     ANHTRRLRKAASELTMSVCQHDAFHDD"
     gene            29215..>30311
                     /locus_tag="VFB37_10705"
     CDS             29215..>30311
                     /locus_tag="VFB37_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HZO22959.1"
                     /translation="MDPGPAWLHGEDRPAQIAVPVLSLLWLLALLACPAGALAQTAGT
                     VGLAQQPAAVADEPTTVASPAELTSEIGPRTVTVTANAELPIDDSVTTTTSVDREDIE
                     RTPGADRSNSLGMITDFVPGAYVVHDQLHVRGGHQTSWAIDGVEIPNTNIASNLGPQI
                     DPKDIQSLEVERGAYGAELGDRTYGIFNVAPKTGFDRTNQGDLVVSGGNFGQTNDYLS
                     IGSHSDDLAYYASINANRSDLGLQTPVEQILHDSVYGYGAFTTLVLEASPHDELRLVA
                     SARQDAYEIPQSPGDQIRDMQREADAFTILSWARALGSSAVLTSSLFYHYNRADLDGD
                     PHDFPISATDRRSSSYAGGQENLHVRTDHNDL"
BASE COUNT         5239 a         9877 c        10121 g         5074 t
ORIGIN      
        1 cctggctgcg cgcggtcttg agctggtaga tgtacgtgag tacctgagcg actgcgttgt
       61 agagcgatgc cggaatctcg ccgccgatct cgacgctgcg gaacagggct cgagccagcg
      121 gcggcgcctc gaagatcggc accaggctct cggtggcgac ctcgcggatg cgggcggcga
      181 tgaggtcagc acccttcgcg acgacgatcg gcgcgcgcat ccggcgctcg tcgtaccgca
      241 gcgcgacggc gaagtgagtc gggttgacga cgaccacatc cgccttgggc acttcctgca
      301 tcatgcggcg cttggcgacc tcgcgctgca ggttgcggat gcggcccttg atctcgggcg
      361 cgccctcact ttccttcatc tcttcgcgca cttcctgacg ggtcatgcgc aggcggcggg
      421 tgtgctgcca catctgccac ggcacatcca ccgccgcgat caatccgagt gtcccggcca
      481 gcatcaggaa ggcgtagccg gtgaccgaag cggcatggcc gatcgcaacc cgcgtcggtt
      541 ccatgcccag acccagcagc tcgctggact tgctgtgcag gatgtgcacc gccacgagcg
      601 caagcgcggt gaactttgcg agcgccttgc caagctcgac cgcaccgcgc gcagagaaca
      661 tccggccgaa accctccatc gggttgaggc gattgaaatc cggagcgagc gccgagaagc
      721 tgagattcca gcctcccagc agcatcggcg cgctcagcgc agcgatcagc gtcagaccca
      781 ggatcggtgc gcacgccacc agggcctgca acatgtcccg cgacaggctc gcaatcatga
      841 aagtctcatc cagcgattgc tcgcggctga gcgtcagggc cgagctcaac atgccatcga
      901 gctggccgcc catgtggcgg ccgaggaagt tcagcccgcc gccggccgtc aacagcaccg
      961 ccgccgtgct cagctccacc gagcgcggca cctggccttg cttgcgggct tcttcgaggc
     1021 gtttgggtgt cggttgttcg gtccgttccg gctcatgctc tgccatggct cagcctcccg
     1081 cgaccgtcag cttgcgcagc agctcgaagc catccccgag caggtgcgtg aaggtcgtct
     1141 gcacggcagg cataccggcc agaacgatga ccagaccgaa tacgagcgag atcgggaaac
     1201 ccaccgcgaa cagattgagc tgcggggcgg cgcggctcat cacgccgaat gcgacgttga
     1261 cgatgagcag cgcggtcacc ccgggcaggg ctatcgagag cgcccccacg aagagctgac
     1321 ttccccactc tacgagtgtc cacagaccgt cctggcccag tccgtcggtg cccacgggca
     1381 gggtgcgaaa gccgtccacc agcacctcga tcagcacgag gtggccgttg agcgccatga
     1441 aggtgagcat gacgagaatg gtgtagaact ggcccagagc gggcacttcc tcgccgcgta
     1501 gcggatcgat gttgaaggcg aacgacagac ccatactgtt ggcgaggagc tgacccccga
     1561 gcgtcacggc atcgaaaagc agctgcagac tgaagccgag cgcgacgccg atgagcagct
     1621 gctgcacggt cacgaggagt cccgctgccg agaaaggtac gatcgccgcc ggcggcggca
     1681 gcagtggcga gatggcgagc gtgaccgcgc cggcgagcac gatgcggatg cgcgccggca
     1741 cgaaaacagc cccgaaggcc ggagccacca tgaagcaggc accgatgcgc acgaacggcc
     1801 acagcacctg cgcgagccag gtttcgagct gccctgtggt gagcgtgatc atgtgtaagg
     1861 cgtgtcggcg tgtgcgtggc aattgctcag ttgatcaatc cgggaatgct ctcgaacaac
     1921 tcgcgcgtat agccgacgag cgtcttgagc atccaggggc cggcgatcac catggcgagg
     1981 gccatggcca gcagcttcgg gatgaatgac agcgtcatct cgttgatctg ggtggccgcc
     2041 tggaatgcgc cgacgatgag gccggtcacc agggcgacga gcaacagcgg ggcggccagc
     2101 atcagagtga tctcgagcgc gcggctgccg atggtcatga ctgtctcggg tgtcatcttg
     2161 aggctcttca ggagtagaag ctggaagcga gcgtgcccat gaccagcgac cagccgtcca
     2221 cgagcacgaa caacatgagt ttgaaaggca gcgagatcag gaccggcgag agcatcatca
     2281 tgcccatgga catgagtacg ctggcgacca ccaggtcgat gatcacgaag ggaatgaaga
     2341 tcaggaatcc catcagaaag gccgtcttca gctcactggt gacgaaggcc ggcacgagca
     2401 tcgtgagcgg cacatcctgg gcagtcgcga agcccttacc gccggagatg cgcacgaacg
     2461 tggcaatgtc actttcgcgg gtctgatcga gcatgaattt cttgatcgga accagggccc
     2521 gatcgagcgc cacggtggct tcgatcgtcc ccgccgagta cggccggaag gcttcctcgt
     2581 tgacgcggtt gatgacgggc gtcatcacga aaaacgtcag gaacagcgcc agcccgagca
     2641 tcacctggtt gggcggggtc tggcccgcac cgagcgcctg acgcaggatg ccgagcacga
     2701 tgatgatccg ggtgaaggcg gtcatcatga gcagtacggc cggcagcagc gtcagcgccg
     2761 tcatcagcgc cagcacctgc agcgtcagcg tgtacgtctg accaccgccg gcgccgctct
     2821 gcacggcaat cgccggcagt cccggctcgg ccatcaccgc cgaggggacc agaatcgcag
     2881 cgccgcacag cccggcgagg gtgattgagc gggcggcgcg catcatttgc ccaggctgcg
     2941 cagcatgagc gctttgaacg agggccggcc gtcggccatg ccgacggcgg atctggagag
     3001 ctcgatgggc tcggcgagca cgtgcagcgt attcacccgc cccggggcca cgccgaccag
     3061 gatctgcgtc tgacctactt tcagcagaac agcgcgctcc ttctgcccca acgggatgtc
     3121 agccaggaca tcgagcgcat tgccgacccg attaccgaag ctgcgcatgc gccgggccat
     3181 ccaggcgatg acaaacaccg tggcgagcac agcaagcaga gcgagtgtga cctgtccgag
     3241 acccccgaga cctgcgggcg cgatcggggt tgcaggctgc ggtgcggcga acggatgagc
     3301 cgtggcggcc ggtgcatcgg cagcggccgc gcacaacgtg gcgcacagcg atgcggcacc
     3361 gaagagcaca gtcaggaagg tggaagggct cgcgcgcacg cagttcactt caacttgcgg
     3421 atccgctcgg cgggactgat cacatcggtc agccggatcc cgaacttctc gttcaccacg
     3481 accacctcgc catgggcgac cagcgtccca ttgacgaaca catcgagcgg ctcgccggcg
     3541 gcgcggtcga gctcgacgac cgagccctgg ttgagctgca gcaggttgcg gatcggaatg
     3601 cgcgtgcggc cgacttccat cgacagcgtc accggcacgt ccaggatgac ttcgaggttg
     3661 acatcgcggc cggactgcgg ggcgtcatcc tgcaggttgt cgaactccgc ggcgcgcggc
     3721 tcggctacgt tggcgttcat cgggaattcc tcaggttcgc ttggcaatgg cgatcgaagt
     3781 ggcgggcgcg ggcggggcgg agcggcggat ccgctcgtcg accttcacag cctgcagtcc
     3841 tcgggaaaga ccgaagctgc cgcggaagac gggcacgtct tccgccagca gggtcacctt
     3901 gccggagaaa tcacagggca gaacgtcccc cggcttcaga ttcaacaatt gggcgagcgt
     3961 gatcgaggtg cgacccagac ggctgttgat ctgcacctcg gcgtcctcga gatcctcgcg
     4021 caggctggaa gcccagcgct cgtccttctc tgcacggtcg ctcgccacgc ccgcatcgag
     4081 aatctcgcgc agcggctcga gcatcgagta gggcatcgtg acgttgaggt tgcccccgcc
     4141 gccatccagc tcgatatgga agctcgtgat caccactatc tccgagggcg agacgatgtt
     4201 ggcaaaatgg ggattgatct cggcctgcac gaattcgacc tcgaccggcg cgatgtgcgc
     4261 ccatgcctcg tgcaggtcgg cgaacacgct gcgcaacatc atgtggatga tccgctgctc
     4321 ggtcgccgtg aactcgcggc cctcgatctt ggcgtggcgg ccggagccgc cgaagaagtt
     4381 gtccaccacc gcgaagacga gcttcggatc gagcacgatc agggcggttc ctcggaaggg
     4441 attcatcttc accatgttga gactggtggg cacgtgcagc gtgtgcgcgt actcggcgaa
     4501 tttcttcacc tggatgggcg ccacggcaat ctcgggggag cggcgcagca ggttgtacag
     4561 gctgacgcga aagagccgcg cgaagcgatc gttgatcatc tccagtgtgg gcatgcggcc
     4621 gcgcacgatg cgcagctcgc tgccgaaatc gtaggagcgc acatcaccgc cgggctgtgg
     4681 cgccccgttc gtgtccactt ttccagagtc cacgccgtgg atgagtgcgt cgatctcggc
     4741 ctggtcgagt actttgtctg tgctcacggg gcggtccggt tactgcatca cgaagctggt
     4801 gaagtagatc gcctccacct tgtcgggctt gccgccggcg gtctcgatca catggcggat
     4861 cgccgcgagc gcttcggcgc gcaggtgttc cttgccgtca cgtgtgccga gctcggcgga
     4921 cttttgattt cccagcagca gcagcaggtc attgcgcacc gcggggtcat tcgccttgag
     4981 cagcgccacg gtctccggat cgcgcgtcat gacctgcgcg gcaacctgca gaaagcgcac
     5041 cagcgggtcg ccttcgaagt tgacgacgaa cggcggatcg agcggcacat acagcggcgc
     5101 agtgaggggc tccttggcct tctcctgcgc ctgcttggag tgcgcgtcag cctgtggcca
     5161 cagaaagaag gcgccaacgc ccccgcccac gaggacgacg gcggcaacga ggccgatgat
     5221 cagccatttg cttttcttct tgcccggggc gtcctccgcc gccggcgcaa gcacgggtac
     5281 gtccgccatc gcgtgcttca ctccatggac aatgtctatg cacccgcagt ggtgcaacgc
     5341 gcatgccacg gctgtcagga cgctcgtaag tcactaaggc cgaagggatt tgtgagagcg
     5401 ggaagagtgt gaaacgcgtc gcagcgacgg attcttgacg tggggatccg cgcatccaat
     5461 gccagcgact ggcgcagccg aaacccgcgc tcggcgcctc tcaggcgtag gtgtcgacga
     5521 gtccgagact gagaccggag gccgaggtgg tggtgctttc cgtcgcggcg accgcgctga
     5581 ccgctgcggg cgccgtcgcc ctgggcggct tggactgatt gcgcggcgat tcgtgtgaga
     5641 cgcccgcatc catgagtgtc agaccttgcg tcgcgaacat ctcgcgaagc cgcggcagcg
     5701 cctgttcgag tgccgcgcgc gtgtcaggct gtgtcgctcc gaaccacacg ctggcagcgc
     5761 cgtcgtgaac cgaaatatgc acctcgagcg ggcccaggtg ctcgggcgag agctgcaggg
     5821 aagccgattc gatcccctgg tgggccatcc aggtgatctg tccccccagc tcctccgacc
     5881 aggccggcgt tccaacctgg gcccgcagcc cgccgccgac actgttggaa tccggagagc
     5941 tgtgttgcac ggaaaagtgc gaggcaattc ccaggtgcgg gatcgtggga gcggcggtgg
     6001 ccggggcgga gtccgccgag cctgtcgcgg tgttgttgac caacggcgca ctcgttgcat
     6061 cggcctggcc ggttgccggt gtcttgtcct tcgattgcgc tgcagcgtcc tctgcgatca
     6121 gtgtcacgag cgtctgaacg gcggacccat tggcggatgc gggttgtgcg ccagcgccgt
     6181 cgatcgcctc ggtgcccgat gccgtgctcg tggctggcgt cgattggcct gtcggggacg
     6241 gcgctgccat cggcggcatc acggccggca ggccaagtgc tgcggcaata gattgggaga
     6301 cgagcgcaag cgcaaggtcg ccctgaccag cacccgcacc cttcgcatcg gcatccgacc
     6361 cgccatcatc tgccgacaca tccgcagctg aagggctcgc agcggctggc gcatcagctg
     6421 tctggccggc gttgatcgac cgggcaagcg tctgaacgaa attcaacacc ggcacaggag
     6481 cctgcggcgc gctgtccttt gccgctgccg aggcggaagc tggtgttgga gtggcagccc
     6541 gtcgcgcggt gcgggcgggc aatgcgcttt tgggcgctgc aggaagcgat gagccggccg
     6601 ctgccgccgg tgcctcgctg cttgctgccg cgccctcggc cgctgccgcg gaggaagcgg
     6661 ctgccgcggg ggatggcacg gggcctttcg ccggcgcgcg cgccgctgcg ccgcccgatg
     6721 aggtgggcga cttgccgggc gcgctggtgg gcgccgcagc gcgactcggc gcggtatggg
     6781 cggaagcggg atggggcgaa gtgggctcgg cggatgccgc cagcgcgcca gcaggggcct
     6841 tcgcaggtcc tggcgccgga tgcgcggtcg gcgtgggtct gatgttaagc gccacgcaga
     6901 gccagtccat gcgcccaggc gcgctgggag cgttcatcgg cttcgtgctg ctcgcgtcga
     6961 tcgagcgcgc gctcttcctc ggtctgaaag cgcttgacca tctgtccgac ggcctcggct
     7021 cgctgcgcgg cgccctgcca ggtccgcaac tcgccgtccc gctgcgcacg cgcgcggatc
     7081 accacctggt tctgctgccg cagcgcctcg tccaggcggc acagaaaggc ctggtagtcg
     7141 cgcgccagga cgccgtccat gccggactcg gcccgaagcg caaaatcgcg ctggtagccg
     7201 gcgtggtagc tctcgagctc gcgcagcttg gtttcgcttt ctacgaggcg gcgctcgctg
     7261 ccggcgaggg cctcggcgcg gctgcgctcc tggtcatcga ccacccgctg gacgatctgc
     7321 agtctctgtg cgcgcttcat gctcattgcc tatcagacgg tttgcccgtc tcgcccctcg
     7381 aagcctcact ctcggccagg acggcactca aggctgtgag gcttgccgcg aaattcacac
     7441 gctccgtcat atcctgctgc agaaacttct gcacacgcgg ccagagtgtg atggcgttat
     7501 cgacccgcgg gtcgctgcca cgctggtagg caccgatggc gatgaggtcg cggttctgct
     7561 gataggtcgc gaggatctgg cggaactggc gggcgctctg gatctgggcg ggggaggcga
     7621 tctcgtgcat ggcgcggctg accgaggcct cgatgtcgat ggccggatac tggccgctct
     7681 cggcgatgcg ccgcgacagc acgatgtgtc catcgagaat cgcgcgggcc gcatcggcga
     7741 ccggatcgtt gttgtgatca tcgccctcgg tcagcaccgt atagaaggcg gtgatcgagc
     7801 ccgagccctg catcccgtta ccggcccgct ccagcagctg cggcaggcgg gcgaagaccg
     7861 aaggcggata tcccttggtc gcgggcgctt cgccgatggc gagtccaatc tcgcgctgcg
     7921 cctgtgcaaa gcgcgtcagc gagtccatca gcagcagaac attcagtccg cgatcgcgaa
     7981 agtattccgc gatcgcagtc gcaagccagg cgccgtgcag gcgcatcagg ggtgggtgat
     8041 cggcgggagt cgccacgacg accgcacgct tgcgatcctt ggggcccagg atgcgctcga
     8101 caaactcctg cacctcgcgg ccgcgctcac cgatcaggcc gaccacgatc acatcggcgc
     8161 tcgtgtaacg ggccatcatg ccgagcagca cgctcttgcc gactccgctg ccggcgaaca
     8221 gcccgatacg ctggccgcgg ccgaccgtga gcagtgagtt gatggagcgg atgccgacgt
     8281 ccagcggctc agtgataggt cggcgcgaaa gcggattcat gggcctgccg gtcaggcgca
     8341 cgcgcgtctc acactcgagc ggtccaagat catccagtgg gtgggcggcg ccatcgatga
     8401 tgcggccgag aagctccggg ccgacgcgta cggtcccggc gcgctggcgt gggatgacgc
     8461 gagcgctggg gccaaggcct tgcgcatccc cggtgggcat cagatacagc cggtcccctg
     8521 cgaagccgac gacttccgac tcgacgcgaa agccatcgcc ggtgatcacg tcgcagtaat
     8581 cgccgatcga ggcctgacag ccgaccgctt ccagggtcaa tcccaccatg cgggtcagag
     8641 agccctcgat cgctggcggc ggggtctgct cgaggcgacg cagcctgcgg gcgagtccct
     8701 ggcgccagat ctccgcgcgc gaggcatcga gtgcgctcac gtttcgggct cgccagaggc
     8761 aggcgtcggc cgctcaggcg ctcgctcttc gccaagggcg ttggcaatga ccgcactgat
     8821 acgactctcg agtcgcacat cgatctgtga ggtgtcggtg cgcaccaggc agccaccgcg
     8881 tgtcagggtc ggatcttcga cgagggtcca ggctcgctcg ctcgccgggg ccgccagtct
     8941 ttcacgcacg gtggcggcat cctccggatg cagatgcacg cggatctcgc gggcggcggc
     9001 gggcagctgc gcgagcgact cgcgcacgat ggcgatcacc tgtgccggat cggtgcgcag
     9061 ctcgcggcgt gcgagctgct tgcccacggt cagggccagc tgcagcagct ccttctcgat
     9121 ggcggcatcc atctcctgca gcggctttgc gagcagattc agaatcgagt ccagctgttt
     9181 gagccgcgta tcgagcgccg cgtggcccgc ctgcatctcg gcctgcgctc gcgccagacc
     9241 cgcctgatag ccgcgggcct cgctctcctg cagcgcacgg gcgccatcgg cgctcttgcg
     9301 cgccgggcgc tcgtttccga tgacggggcc atccacacgc ggcagcgacc agcgctccac
     9361 ggcggcaggc gcgcgctgtg gatcagacat actcttcgcc ctttccggcg agggcgatcg
     9421 tgccggcttc cgccagcttg cgcgccgtct gcaggatctc cttctgcgcg gcctcgacct
     9481 cggagatgcg caccggtccc ttggattcca gatcgtcctt gagcatctcg gcggctcgct
     9541 gcgacatgtt cttgaagatc ttctccttca tgacatcctc gcagcccttc atggccagca
     9601 gcagcttgtc gctcgggacc tggcgcagca gctcctgcat gctgcgatca tcgatgtcga
     9661 gcaggttatc gaagacgaag atcagatcct ggatgcgcgc gctcagagcc tcgtcggtcg
     9721 cggtgatctg ctccatgagc acgctttcct ggctcggctc gaggttgttg atgatgttgg
     9781 cggcagcctt ggcgccgccc agcgtggagg tgcgatgcgc gcccttgccg gcgaactgct
     9841 tctccatgat gtcgtccagc tccgtgagcg cactcggctg cacgccatcg agcgtggcga
     9901 tgcgcaccag cacgtcggag cgcatgtccg gtggcaggtt ggaaaggatg tcggcggact
     9961 ggtcgggctc gaggtaggcg agcacgatgg caacgatctg cgggtgctcg agccggatga
    10021 gctcagccac tgcgcgcgca tccatccact tgagcgcttc caggcccttg gtggagcggc
    10081 cgatgctgat ccggtcgatg atgttggccg ctttttcctg gccgagggcc tccaccagga
    10141 ccttgcgcaa gaattcttcg gtgccgacgc ctacggaagt ctcgctctcc acctgagagc
    10201 tgaactgcgt catgattccc tggacttcct cgcgcgagac gttggcaagg gtcgccatgg
    10261 ccgagccgat gcgctgcact tcgcgcgcgc ccatgtgctt gagcacctgg gctgcttcct
    10321 gctcgccgag tgtcagcagc aggatcgcgg cacgctcggt gccggagcgc tcgctgcttt
    10381 cgcgcttgtc actcatccgt ggctacccag ccgcgcacca cctgtgcgac gcgtttcgga
    10441 tcctggctga ccagatttct cgcctgtgcg atctgctgct cgtgcgcgac cacggtgggt
    10501 gatcggccgc cgaccagagc cggggctgga gcaggctcgg gcgcgcgctg caaggcgggc
    10561 cgcgccggcc ccatcagcga tttgaccaga ggacgcagta ccgagagcac caggaccagc
    10621 aacaccagca agccggcgag gatcttggcc aggtcgcgaa acagcggcga ttcccagaac
    10681 ttcggcttct cgagctcgcc atcgttgctc ggcggagcct cctgcgtgaa ggaggcgttc
    10741 acgacattga cattgtcgcc gcgcgcttcg tcgaacccga cggcgtcctt gacgagctgg
    10801 gtgagatgat cgagctggtc cttcgaaagc ggcgtctcgg tggccttgcc gtccttgcct
    10861 actgtacgca ggttgtctac gagcaccgcg accgtgagcc gccgcaaccg gcccgccggc
    10921 tggcgcgtgt aatccagggt ccggtcgatc tcgaaattct tggtctcgct cttcgaggtg
    10981 ctgtccgtgc ctcctgcgcc ggagccggcg atcgggctcg tggcagccgc tgcggcggcc
    11041 gcaccggact gagccgttgc ggcagcgggc ggcttgggag ccgctgccgg tggcgtgctg
    11101 ccgctgcccg ggggcagcgc gactccgctg gcgggcggtt gattgctgag cgcgcccggc
    11161 acgccctgcg cgcccgagcc atcgcggctg gtctgctcgg agctctgctc gctgcgaacg
    11221 atctgactgt cgggcttgta ctgctcgcgc gcttcctcgg tcacggacat gtccatctgc
    11281 gcgacgacct gggcgcgcac gcgccccgag ccgaccacag gcgacagcag cgcctcgatg
    11341 cgctgcgcgt aatcgtcctc caggcggtga atcatgtcgt actgctgatc gcgcaggccg
    11401 aactgatcat ggccgtcggg cgaggagagc aggcggccct gctggtccac gaccgtgacc
    11461 tggttggcct ccagatccgg aatgctcgag gccacgaggt tgacgatggc ctggatctgc
    11521 tcctgctcga ggcggcgacc ttgcttgagc tgcaggaata cggaggcact ggcccccttg
    11581 tggtcgcgca cgaatgccga ctggcgcggc acagccagat gcacccgcgc accctccacc
    11641 ggacgcagcg ctgagatggt gcgcgcgagc tcggtctcga gtgcgtgttg atagcgggcg
    11701 ttctccatga actggctgac accgaaaccc ggatccttct ccatggtggc gaagccgccg
    11761 ccgccttcgg gcagaccctg gccggcaagc ttcaggcggg catcgctcaa gcgctcggcg
    11821 ggtacctcga tggagttgga gcccggctgg atgcgataag gaatctgcgc agactcgagc
    11881 gcctgcgtga tctgcgcctg atcctcggtc ccgaggttgg catacagcag gctgtaggtc
    11941 ggcccacgcg accacaggac caccgtgaca ccggccgcga ccgccatcgc gatcccgacg
    12001 agcagcagca atggacgcag gccggcaggc aggccggcgg gcgctgggag ctgggcaact
    12061 tcagcattca tggatgacaa tcaatcccgc tcagatctgc atgttcatga tgtcctggta
    12121 agcgctcacc aggcggttgc gcacctcagt gagggcgcgg aaggagacgc tggccttctg
    12181 catctccagc atcacctggg gcagctccac ccccggcgcg ccgcgctgga aggcatccgc
    12241 gagctgcgtg gcgcgcagct cgctctggtt cacctgatcg atgcccttgc tgagaatgtt
    12301 ggcgaactca ctcggaccgg cctgcgcggt cgggttggtc ggggcggcgc ccacccgcgt
    12361 ttgattggac agcgagcgga tctgcgccag aacgcggtcg atctccatct gactcatggt
    12421 gtcactccgg tgtaggggtc tcaggctgcg ggcacgtcga tgccggcttc gcgcagccgc
    12481 gcgagcttgt atcgcagggt gcgctggctg attccgaggc gttcggccac ttcacggcgg
    12541 ttgccctgac cactgcgcag cgcatcgaga atgaggtcac gctcggtctg acccagggag
    12601 ctcgccaggg agccgctctg cggcgccacg gcgggggcgc tgtccagggt cgggttaact
    12661 tgcgccggcg cctgcgtgac tgcgccttcg ttggcaagct cgaactggat gtgctcgggg
    12721 cggatgaccg gcccgttgac caggatcagc gcgcgctgca acaggttgtc gagctcgcgc
    12781 acgttgccgg gccaggaata ggtgagcagc agatgcgccg cttgcgcgct gagcgccggg
    12841 atgagcgccc ccgggcggca gcgcgagccc agaagctgca tggccagcgg cagcacatcg
    12901 tcctggcgca ttcgcagcgg ggcgatcgca agcggaaaca cgttcagccg gtagaagaga
    12961 tcctcgcgga agcggcccgc ggccacatcc tcgcgcaacc gacggttggt cgttgcgagc
    13021 acgcggacat cgagcgcaat cggtgtgcgg ccgccgagcc gctccacctc gcgctcctgc
    13081 aggatacgca gcagttttgc ctgcaagccg agcggcatct cggtgacctc atcgaggagc
    13141 agcgtgccgc cctgcgcctg ctcgaacttg ccgggatgcg cggcgtgcgc accggtgaac
    13201 gcgccacgct cgtagccgaa caacatcgcc tcgagcatgt tctcggggat cgccgcgcag
    13261 ttgaccgcga cgaaaggctg cgcgctgcgc ggcgagcggc gatggatgta acgcgcgagc
    13321 acctctttgc cggtgccgga ctcgccgacg atgagcacgg tgcaatcgct cgcggcgacg
    13381 cgccgggcca gctcgagcaa gcgagtggag ctcgtcgcgc aggcgaccgg ctccggctcg
    13441 atgcgtgcca cgcacgcgcc cgccgtctgt tggtcgatcg gatcttcagc cattcacgcc
    13501 tcgcacgcac cggtcgatgc aggtaggcgg gaggttgcaa aaggcgtgcc gggcggcaaa
    13561 gcgtgaaagc gatcacaagg ccggcacgag catgccgcgc gagcgtcaat cttccggcgg
    13621 gagctgaggt ccgaagaatc gcggccgcga tgccttccga cgaaccagcg gtctaggcgt
    13681 cctcggcgct cgaggccatt cccagcttgc gcatcttctc gacgagcgtc gtgcggcgca
    13741 ggttgaggag cctcgcggcg tgcgccacgg tgccgcgtga acgctgcaag gcctgttcga
    13801 tcagcgcacg ctcgatggcg agcagatgga cccgcaggtc gagcccttca tcgggcaaaa
    13861 ccgcttgcgc gtccgcaact gcggcgccgg ctgaggacag gcactctgcg ggaaccagct
    13921 ccgcgctttc gagcacggac agcgcggtct cctcgctgac gatcgcctct tcgatggcaa
    13981 tcgactgcga gggatcatcg agcgagctca cgcagagcga gctgtcgaat tccgtcccct
    14041 gcagcttcca gtcttccggc ggccgatatt tcgggggcag gtcgcggact tcgacgacgc
    14101 ggcccgcgca cacgatcgag agacgctcga tgagattggc cagctcgcgg acatttccgg
    14161 tccacgggca cagggcgagc gcgccgagcg ctcgcggtga gagctgcaca cggccgcgtc
    14221 cctcggcgac gttgctggtg atgcagtcgc gcaccagcat cggcagatcc tcgatgcggg
    14281 cgcgcagcgg cggcatctcg atgggaaata cgttgagccg gtggaagaga tcctcgcgga
    14341 agctgccgcg ggcgatcgat tcctccagat tacggtgcgt ggcggcgatg atgcgcacgt
    14401 tgcagcgcac cggcacgtga ttgccgacac gctcgaaaac tctctcctgc aacacccgca
    14461 gcagcttgac ctgcatcgtc gggctcatgt ccccgatctc atcgaggaac agcgagccgc
    14521 cctcggcgat ctcgaagcgg cccttgcggg cgctcaccgc gccggtgaat gcgccgcgct
    14581 catggccaaa cagttcggat tcgagcagct cggcgggaat cgcgccgcag ttcacggcga
    14641 cgaacggccg gctgcggcgc gggctcaagt catggatggc gcgcgcggcg acttccttgc
    14701 ctgtgccgga ttcgcccagt accagaaccg tggaatcgtg cccggcgacc tgccggatga
    14761 gcgcgatgac gtcgcggatg acgacggagc tgccggtggg aatacggatg cgatggtctt
    14821 cgaacgcgac cagttccggg accatcgggc ggtccgcgtc cgattgcgcc gcgtcggccc
    14881 tgagcagctc ggggatgaga acggattccg ctgccgacat gcacactccg aggcccgatc
    14941 gagggtgctt atgttgatga gtaccacccc gcaatgctgt gaagcattac cggttgaggc
    15001 cgtggtgcag catgggcttc gtgactgcag tcacggaaag ccagatctga agcggtacgg
    15061 attgtaagac tagccgcggc gcggctgccg cgcgccggca atctgtcgcc ggtgccgccg
    15121 gaaggccagc ttctcgatga ggtcggcgac cccctccccg aggggcagga atttgacctt
    15181 gatgtgcccg tcgggcgtca cattggtgac ccggccggcc aggcgcagcg gctcggccag
    15241 gcagtcgcgg aggtagatct cggcgatacc ctgctcaccg gcttctggca aggggccact
    15301 ggccctccag acggccccca acgcattgaa tcgcaccggt gagggcgccg ggcgggggcg
    15361 actgttcgcc aggatctggc ccacgaggtc cagaagcaga ttcactttca ggtcgagccg
    15421 cagcagctcc gcggcatggg gggaatcgtc cggcttctcg accggaccat gctcttccat
    15481 cgcatcccag gcctgcagca gtcggatgtt gcgctcatcg aagctcgccg cgagatcccg
    15541 atccaccgga gtattcagcg gccgccacag cacgggcagc acgtcctgaa aggccagctc
    15601 gtcgtacagg actacagtgt cggacccgtc atacatacat tggccacgcg cagcggattt
    15661 cccgcagaag atgcgacact tcgtcgagcg tcgcaacccg atcgtgcaga ctcgccgcgg
    15721 caagctgtcg ggagaggtag tcgcaaagat cgtccaggtt caccgcgtag gcatctccgc
    15781 ggtgcagatc gagactgttg cgcagcgttt ccaggagctg cacggctccg tgcaggagct
    15841 cgcgcctctc ggaaccctca ccgctgtgca gctgccggcg cgcctgggcg atgtgctcga
    15901 gcgcgctttc catcagcatg ggtgcgaacc cgttggagtg cgccgcggcc gggcccagcc
    15961 gggcgaccga ggagtcgaag gcgcaggttc tggagggcgc ggctgccggc atgaggtcgt
    16021 accgaggcgt taaggatctc tctggggaag atagcggccg ctgaggccct gacttgagaa
    16081 ggcgcgatcg cgcgggaaca gccgcggatc gaggcgggct tcacgccggc gctgggtcgg
    16141 ccggcgcggt tccctagccg ctggcgcggg tgacgggcgc caggttggcg gcggtctcgt
    16201 cgagaaagaa gcggcgcacc aggatggcct ggcgcagctt gcctttgcgc acgtaggcct
    16261 cgtacagaac atccttcatg acctcgagca cgattccagc ctcgtagctg tcgagcagcg
    16321 gcgggttcgg gcgcggatcg ctctcggatc cgaacaggat ggtcttgagc ttgccgtagg
    16381 tctcggaggt caaccctgcc agatcgcggg cggtgttgag ttcatcgaac agacgcagct
    16441 tgccggcttc gcccagatcg gcgaacaccg cctgcgcagt ccgccggcac atggaggcga
    16501 aagcattgaa agcgccagcc gagaagcagg cgaggccttc ccggaagaga atctccacat
    16561 cctgaggcag atatgtgaag gagaacttct cccgcgcgcg ctccacttcc gtgaactgag
    16621 gcgcgagctc cacgcgcgaa gggccataca tgcgaaccat gaagcgggtg aagattgggg
    16681 ctccacacgc atcgcaacga tagaccaccc cgatctgctt cggccgatgg gcctgcagat
    16741 cctcgaagcg cgggacggcg ctcggcgtga cgtgcgagac gacttcgcaa tgcggacagg
    16801 ttactgccag gttctgctcc tgatcgtgga tgagctggct cgacgaatcg atgtgtacgg
    16861 tcatcgggtg ctggcgtcag ctgttgttct tttccgtgcg cgcagcaaga gtctagctca
    16921 tcggccggcg gccgaggaat ctatcgcgcg cggactacgt tatgtcatgg cccgcgctct
    16981 tcgcaccggc gtcgccccga tgttggacag cgcgggctgc tgcccggatc cacatgcagt
    17041 ctcatctggc aaacagctca cgcataccgc cggcaatcag catcgataca tccaccagca
    17101 cgcgcggcag cgccagtccg ccgcgagcca ccaggtactt cggcacccag acaggctcga
    17161 acttgccctt gtagcgacgc aagccctcga agttgtagaa atgctcgcca tagcggaaca
    17221 cgaaattgcc tacgcgatgc cacgcgggag ccagtggatg cgtctcgagc cccgcgagcg
    17281 gggccattcc cagattgaac cagccatagc cttcctgacg gccccatttc atcagctcga
    17341 tgaacagata gtccatcgcg ccgcgtggcg catcgttgcc gaagcgcatg aggtccaccg
    17401 acagctcctc cttcgtgccc gacacccaga ggttggcgaa ggcggttgga ttgccatcgc
    17461 agcgcaccac tgccatgggg aaattgcgca gataccccgc cgagaaggca cccaccgaaa
    17521 agcgcttctc gcccgtggac ttgctcgcca gccacgagtc ggaaatcttc tgcaggtccg
    17581 gtagcaatgg cgcgacctca tcgggccgga cgacctcgaa ggttgcccca tcgcgcaccg
    17641 cccggcgatg cgcctgtctc aggtcagccc gcgcggcgcc ctcgagtgag aactcggtga
    17701 gcggcacgcg cgcctcctcg ccaatcttga gcgcggccag accgagatcc acgtacagcg
    17761 gcaagcgagc gccgctggcc tgatagaaca ccgtctgtcc cccatgatgg tcggacatct
    17821 cgcggaagcg ccagacgagc tcttcagcgc ccgcctcggg cccgactgga tcgccgagtg
    17881 caatccagct gcgaccggcg atctggtaca tcacgaagga gtcgcccgca tcgttgaaca
    17941 gcagccgctt gtcacccgtc agcgcggcat tcgcgaccgt gagatcgcaa caggcgatca
    18001 ccgcccgggc gcgctccaga tcctgcggcc cggcgaccgc cggcggcggc tgcgccggtc
    18061 tcaacaagtt ctgcagcagg taggccgcca acagcaccac gaccaccagc gaggcgcgca
    18121 gcatgcgggg cgcgttgcca tcgagcgcga aggtccacca gagctcgtcc gaatactcga
    18181 cgcggcgata agcgatgaag cccacccaca cggccaacgc aatgacaccg acgatgctga
    18241 cgatccacac cggcgtcacg cgctcggaga ggatggccgt aggacgatga aatgcgttgc
    18301 gccccagcgt gagcacgccg agtacgagcg cgagaatggt cgcttcctcg aagtcgaggc
    18361 ccttcaacag cgaggcaatg atgccggcca agagtagcca gacggcaatg tggtacgccg
    18421 tgcgcacgcg acggaagagg gcgcgcgaga gaatcagcag cccaagcccg atcacgctgc
    18481 cggccaggtg cgagacctcc accaccagca acggcaggat tcgatacaga acctgcaggc
    18541 gagtgtcgat tgccggggtc gtgcccgaga gcagcaggat cgcgcccgcg aggaacgtca
    18601 gcgcgcctgc gatctgcggc accaccggcg tgatgtacat cgcggcgata tcgcgcgccc
    18661 gtgcgagccg cccgcgctgc tcgagcagtt cctccgtgcc gaacagcaag gtgccgacca
    18721 gcaggggcac caggtaatac accccgcgat aggcgagcag agagcccagc agcgcatcgc
    18781 ggggcacgtg cggcagtgca tacagaatga tggtctcgaa tacgccgatg ccgcctggca
    18841 cgtgactgat gatcccgacg atcacggcgg cagcataggc gccgacgaac gcaatgaagc
    18901 tgacgtgtgc ctcggccggc aacagccacc acaacactgc gctcgagaga ctcagatcga
    18961 tcacgctcag cacgatctga gtgagtccga tcgcggcacc cggaggacgc agtgcccagc
    19021 cccgaatctc cagctcacgg cgcgcggtcg aggcccacaa tccgtaggct gcgaccagag
    19081 cgagcagcac gacacccacg gccatcgacc agccctgctg cagatgcaac acgctcgcgg
    19141 actgctccgg cgtgatgaac aacgagatac ccgcgagcgt cgccagtccg atgccgatcg
    19201 acagactgca gaacgccgag atcgtcgcca cgtccacggc cgtgaccccg gcagtggagt
    19261 acagccggaa gcggatggcg gctccggtga aggccgcaaa ccccagcgtg tggccgaagg
    19321 agtaagcaat gaaggaggtg aagagtatcc gccgataagg caggactctc ctgcggtaca
    19381 tcagcgccag cgcatcgtag gtgctgagca gccagtagct ggccgctgtg aatcccagcg
    19441 cgatgagcac gttgcggcgc ggaatccggt gcagatgcac gaacacgctg tggacgtgca
    19501 gccgcgccag ctcgttgtgc aaaacccaga cgacgaggcc gaacaccagc agtgcagtga
    19561 gcgggcctat ccagcgcagc gcgctgaaat ccttgctcac ggacagggag gccgacggat
    19621 cggacaagac agcagccggc tcttcagcgt gtcaggggct tgtacttgat gcgatgcggt
    19681 tgcacggcct catccccctt gcggcgcctg tagtcctcca tgtactcctg atagttgccc
    19741 tcgaaccaga ccgcctgcga gtctccctcg aatgccagga tgtgggtggc gatgcgatcg
    19801 aggaaccagc ggtcgtgaga gatgaccacg gcgcaaccgg ggaagttgac cagcgcctcc
    19861 tcgagtgcgc gcagggtctc gatatccaga tcgttggtcg gctcatcgag cagcagtacg
    19921 ttgccgccgg atcgcagcac cttggccagg tgcacgcgat tgcgctctcc accggagaga
    19981 tctccgatcg gctgctgctg ctggctgccc ttgaagttga agcggccgac gtagccgcgc
    20041 gagggggtct cgtagtttcc gaccttgagc atgtcgagcc cgccggagat ctcctgccag
    20101 acggtcttgc tgtcatcgag cgactggcgt gactgatcga cgtaggcgag gcgggtcgaa
    20161 gggcccatgc ggatctcgcc cgcatcggct ttctctgcgc cggtcagaat ccggaacagc
    20221 gtggtcttgc ccgcaccgtt cggcccgatg atgccgacga tgccgccgcg tggcaggttg
    20281 aagctcaggt tgtcgaacag cagccgctcg ccataagcct tgcgcaggcc cgcggcctcg
    20341 atcaccagct ctccgagccg ctcaccgggg ggaatgtaga tctcgttggt ctcgttgcgc
    20401 tcctggaact cgcgcgaggc cagctcctcg tagcgctgca ggcgggcttt gctcttggcc
    20461 tggcgggcct tcgggttctg gcgcacccat tcgagctcgc gctcgagtgt cttgcgcagc
    20521 gcctcgcgct cgcgctcctc gagcgcgagc cgctgctgct tctgctcgag ccaggaggaa
    20581 tagtttccgt gccaggggat gccgtgcccg cggtcgagct cgaggatcca ctccgcgacg
    20641 ttgtccagaa agtagcgatc gtgcgtgacg gcaatgaccg tgctcgggta ttcctccagg
    20701 taacgctcga gccaggcgat cgactgcgca tcgagatgat tcgtcggctc atccagaagc
    20761 aacatgtcgg gcgcagaaag caacaatctg cacagggcca cgcgccgctt ctcaccgccg
    20821 gacagggtgg caatggaggc gtcccagggg ggcaggcgca gggcatcggc tgcgatctcg
    20881 agcttgcgct cgagctccca gccgcccgcg gcatcgatgg catcctgtag gcgagcctgt
    20941 tcctccagca gcgcgttcat ctcctcatcg ggcaggggct cggcaaacct gtcgctgatc
    21001 tcgttgaatc tttcgatgtt gcggaacgct tcgcccacgc cttccatcgc tgtggtgcga
    21061 acggtcttct gcggatcgag ctgcggctcc tgcggcagaa agccgacgcg gattccgctc
    21121 tgcggccgcg cttccccgtc gatgccggtg tcgaggccgg ccatgatctt cagcagggtg
    21181 gacttgccgg ccccgttcag gccgagcacg ccgatcttcg ctccgggaaa gaaggacaga
    21241 ctgatgtcgc gcaggatgac ccgtttcggg ggcaccacct tggatacgcg attcatcgtg
    21301 tagatgtatt gcgccatgga actcgtgtaa tgaactgtaa gaggtttgcg ggccgggcat
    21361 tatggtcgag ccggcccccg cttggcgatg gtggcgaatg cccgtgtgct aggctttcgc
    21421 ctccaggagc tgcaccctcg aaaatcgagc cggctgcacg cagcaagtga acgccgatca
    21481 tcaagacgct cgattcccgg gcggcccccg cagcagcctc ggggtcgtcc tgagcgagcg
    21541 actcgcccgc tatgccttcg gcaacggcca ccccttcggg cccgaccgcc acgccgcatt
    21601 cctccgggag ttcgaatcgc gcggcctggc tcgccgtgta cagatcatcg agccccgcga
    21661 tgccaccgcc gaggagctgc tccagttcca cacccccggt tatgtcgatc tggtgcggac
    21721 gcggtccgcc gacggtcacg gcttcctgga ctatggcgac acgccggcat ttcgcggcat
    21781 gtacgaggcc gcgaccgtcg tggtcgggtc cacgttgaat gctgccgagg cgatcatgtc
    21841 tggacagtgc cggcgcgcgt tcgttccgat cgcgggactg caccacgccg cgcgggatcg
    21901 ggcggccggc ttctgtgtct tcaacgactg cggggtgctg atcgagcggc tcaagaggca
    21961 atgcagcctc gagcggatcg cctatgtcga tatcgatgca caccacggcg atggggtgtt
    22021 ctacgccttc gaggatgatc ctgcggtgat catcgccgac acccatgagg acggccgata
    22081 cctgtatccg gggaccgggc tcgccagcga gacggggcgc ggtgaagcgg tcgggacgaa
    22141 gctcaacctg ccgctgcagc cgggcgccga cgatggcgtt ttttcccgcc tctggccgca
    22201 tatgctcgcg catctggagc gcttccaacc gcagttcatc attctgcagt gcggggccga
    22261 cagtctggag ggggatccga tcacccattt gcgcttcagc ccgaaaaccc atggccgggc
    22321 agcgcgtgag ctggccgcgc tggccgatcg gctgggccat gggcggctcc tggcgctcgg
    22381 aggcggaggc tataaccgca ccaacctggc gcgggcctgg accgccgtcg tggagagtct
    22441 gctcagctga gccaggcgcg cggaggccct ccggacaggc tccggggcct tccggtacaa
    22501 tcagcggccc ggtctgtgtt atcccgctca agggcttcta tgttccgcaa atcacctgat
    22561 ctcgccgcgt tcgatcctga gcttgcgcgg gccatccagg gcgagcgcca gcgccaggag
    22621 gaccacgtcg agctgatcgc ctccgagaat tacacctcgc cgcgggtcct cgaagcgcag
    22681 ggctccgttc tcaccaacaa gtacgcggaa ggctatccgg gccggcgcta ctacggcggc
    22741 tgcgagtacg tggacatcgc cgagcagctc gcgatcgatc gcgccaagaa gctcttcggc
    22801 gcggcctacg ccaacgttca gccgcactcg ggctcgcagg ccaatgccgc ggcgtactac
    22861 gcgctcgtgc agcccggaga tccgattctc ggcatgagcc tggatcacgg cggtcacctg
    22921 acccacggcg cgaaggtcaa cttctcgggt aagtatttca aggccacgca gtacggcatc
    22981 cggcccgaca cgggcgagat cgactatgag caggtgcagc ggctggccga ggagcatcgt
    23041 ccacggatga tcatcgccgg cttctcggcg tattcacgga tcgtggactg ggcgagattc
    23101 gcgcagatcg ccaaaagcgt cggcgcctac ttcgtcgtcg atatggcgca cgtcgcagga
    23161 ctcgtggccg cgggcctcta tccgaatccg atcgagcacg ccgatgtcgt gaccaccacg
    23221 acgcacaaga ccctgcgcgg tccgcgtggc ggactgatcc tggcgcgccc gaacgatgag
    23281 atccacaaga aactcaactc gctggttttc cccggcacgc agggcggccc gttgatgcat
    23341 gtcatcgccg ccaaggctgt tgcattactc gaggcgctgc agccggattt caacgagtac
    23401 cagcgtcagg tcgtggcgaa cgcgcgcgcg atgtgcgccc ggctgatcaa gaagggtttc
    23461 ccgatcgttt cgggaggcac ggacaatcac ctgttcctga tgagcctggt ggacaggaag
    23521 atcaccggga aggatgccga tgcggccctc ggccgcgcca acatcacggt caacaagaac
    23581 gccgtaccca acgatccgcg cccgccgatg atcgcgagcg gactgcggat cggcacgccc
    23641 gcctcgacca cccggggctt caaggagcgc gagatcgagc aggtcgccga cttcatcgcc
    23701 gaagtgctcg atgccgaggg gtcggaagcg gccgtgcgcc gcgtacagcc gcaggtgttg
    23761 gacctgtgcc ggcgtttccc cgtctacggc tcgtaaccga cagcggaacg ccgcgtgcac
    23821 tgtcctttct gcggccacgt cgaaaccaag gtcaacgatt cgcggctggc cggtgagggc
    23881 cggcagatcc gccggcgccg cgagtgtctc tcctgcggag agcgcttcac caccttcgag
    23941 actgccgagc tgctgatgcc cgtggtcgtg aagggcaatg acgtgcgcga ggcctacgac
    24001 gaagcgaaac tgcgcatcgg catggagaag gcgctggaga agcggccggt ctcgcgcgag
    24061 gcgatcgatg aggcgctcag ccgcatcacg caccgagtgc ggatactcgg cgaccgcgag
    24121 gtgccttcac gcatcatcgg cgagtacgtg atggacgagc tgcgtcagct cgatgaggtt
    24181 gcgtacgtgc gcttcgcttc ggtctaccgg cattttcagg acgtcgaggc cttccacgag
    24241 gagatcaagc ggctgcgcag ccagcgtgcc gcgcccgcgc agcatccgac ccgcggctcc
    24301 aaacgaggct cggccaagga ggccagcaag gatcagctgc cgctgttgcc ggcggacccg
    24361 ccggccaacg ggagcaagca ggcgtgatca ccgacttcga ccgctacgcc atgaatcgcg
    24421 cgttggcgct cgcagagcgg ggactcgaga cgaccgatcc gaatccgcgt gtcggatgtg
    24481 tgatcgccaa gaatggccga atcatcgggg aggggtggca tcagcgcgct ggtgaggctc
    24541 atgcggagac ggctgcgctg cgtgccgcga gcgaggccgt gtcaggttcg accgtgtatg
    24601 tgacgctgga gccttgcagt catcatgggc gcacgccgcc ttgcgccggc gccctgatcg
    24661 aggctcgaat cgcccgcgtg gtctacgccg tacaggaccc ggacccgaga gtcagcggcg
    24721 aaggcgccgc agcgctgacc cgcgcggggg tgatggtcga gaggggtttg atggaagcgg
    24781 aagccaccga gctcaacatc ggcttcatga agcgtatgcg acatggactg ccctgggtgc
    24841 gtctgaagct ggcaatgagt ctcgatggac gcacggcact cgccgatggg agcagccagt
    24901 ggatcactgg cggaccttcc cgcgaggacg tgcatcgctg gcgcgcgcgc agctccgcac
    24961 tcatgaccgg cgtcgggacc attctcgccg atgatccgca actcaatgtg cgactgccgg
    25021 ggagcaacca tcgacagccc ctgcgggtca ttctcgacac ccggctgcgc acgcctcccg
    25081 atgcgcgcct gttctccgtg gagggcgagg tgttgattct gactgccacg accgtcgccg
    25141 aggccgcaga agcgctggcc gccggcggtg ccagtgtcga gatcattccg gcctttgcgg
    25201 gcttgctgga tctgtcggca gtactcgccc gactgggtga gctcgagatc aacgaggtgc
    25261 tggtcgaggc gggaccgacg ctctccgggc agctgctcac cggcatgttc gtcgacgagc
    25321 tgctgctgta tgtggcgccg aagctgctcg ggccgcaggc gcggccgctc atcaatctgc
    25381 cggaagtgca gtccctgcag gaagcgcgcg gattctcgct cttcgatgtg aagcgcacgg
    25441 gcgaggatct gtgcctgcga cttcggccgc actgaatcgc aaggggccgc gggtgttcac
    25501 tggaatcgtg caggatgtgg ggcgggtcaa gtcgctcgag cgccgcggtg gggacgttcg
    25561 gatcaccatc gagctcggca agctcgacat cggtcttttc catgttggcg acagcatctg
    25621 tgtgcagggc tgctgtctga cagccgtcga gctgcacggc aagtcgttcg aggcggatgt
    25681 ctccacggaa tcattgcgcc tgaccaccct gggctcgctc gagccaggct cctccgtcaa
    25741 tctcgaagct tcgctgcgcg ctggcgatcc gctgggcggc cacctggtct caggtcacgt
    25801 cgatggcatc gccaaggtcg tctccatcag tggggatgcg cggtcgcaga ggattcggtt
    25861 ctctgtgccg cgcgagcttg cgcgttatat cgcgaggaag ggatccgtgg cgctggatgg
    25921 cgtgagcctc acggtcaacg aggtggaggg cgaggaattc ggcgtcaaca tcattccaca
    25981 cacgcaacag atcacgacgc tgggccggct gacaaccggt gcccgggtca accttgaagt
    26041 ggaccaggtg gcgaggtaca tggagagact ggcggaatat tccgcggact tccgtgggac
    26101 gctcagataa gccctaagta tcaaaaagat acggtgcatt agattccgca tgattggcga
    26161 gcggccgcga cggccgcctg ctcgtggggc ggccccccct gagatttgct gcattgggtc
    26221 cgattattgc ggcgagcgct gtcgccctgc gaatgcgagc gcacacaaat gacgccgacc
    26281 ggcccgacac agaaacctat tggggaccct cctccggatc cttctccgac accgccacct
    26341 ggaaccccgc catcccgagg gccagatccg ggtgctccga caccgccggc gccggtcgtc
    26401 cccgaccctc ctgaagcacc aacctcggcg cccccataac cggctcgggg ctcgctgcat
    26461 cgagcgcgat gctcatcggg tagcgcaacg cagctgcgct tcgggtgcca tcggcgaagc
    26521 cgcatttgtt ggggctgccg ccggcgaggg tcttgacgac gccggccgga ttgatcgggc
    26581 ggatggcgca attgctgctg tcgggtaagg tgaacgagtt gcagcttctc agctcggctt
    26641 cgccgcttcg aacacgaaca cgcgtgaagg accactcgga ctcagcttgg ccatgcgctc
    26701 gaagcggaat ccgagctcgt tgaggatgcg aatggaggcg gtgttgtccg gcgccgtcag
    26761 cgcaaccaat cgagtcagtt gcagctcctg cacggcgtag cgcaaggtgg cggcagctgc
    26821 ctcccgcgcg tacccgtggc cgcgtgccgc cggcagcagg gcgaagccca gctcgacatc
    26881 ggggtgagta tcacggcgca ggagtccgca cataccgagt acgggtccgc catccttacg
    26941 ttcgagcgcg tacagaccga agccgtagcg cgagtagctt gcgccggggc tttcccggat
    27001 ataacgctgc gcgtcggcga tgtcgtgcac accacggtcc ccgatgtggc gcaggaattc
    27061 ggggtcagtc aggagctcca ggatgaaggc ggcgtccgcc ttgacgaatt ggcgcaggcg
    27121 cagtcgcgcg ctctcgatgg cacaggtcac gacagacatg agcgcatcct atgctactcg
    27181 ccagtcttga agacaaactc gtggctctcc gcctgccgtc cgccggcgcg aatccacgag
    27241 gctcgctcgg catcgatcgt caggaaccac gtctgacgtt tgcgcacgcc gccggtgctg
    27301 gcgaaagcac ggcaatcgag caccgccgcg cagccgccgt gctccggatc gcggacggac
    27361 tcatatcgaa ggatgcgcgt gccggcttcg cgtgcgacgc gggcgaatcc ctggcagtca
    27421 ccgtactgat tcgggttgtg ccatttcttt gcgtcgcggc tgaagggttt ggcgcgaaga
    27481 tccacggcag ggccgcaagc gctggcctga aagatcgtgt gcggcacgcc gtcgagccgc
    27541 gagaggccat gactgtcggc aacgaagcgc caacgccagt aaccgacctc ggcgcagctc
    27601 gtgcggaggg cttccgcccc gtaccagact ccgggatctc cgaaagcgcg gaagcgggag
    27661 ccattcggcg ggggcggggg ataccggtag ggcgtgtaga ggaggtagtc gtagcgccgg
    27721 caatcggcgg gaatcggcgg cttggcctgc tctaacacag actcgaggat ttcctgctcc
    27781 tcgagcgagt cgacgagcgc acgcgtggcg acctggtgtt gggcttcgac ggcacgccag
    27841 aggtccagcg tgcaggcagc cgcctcagat gcgaccgcgt gcgccgtcca ggtactggag
    27901 gacatggatc aggccttccg cgctcaggat cctttcggcc ggctcacctc cgagcgcgtc
    27961 attgcgcgaa tgcagccagt cgcgcatggc ctgactcttg ccgccggtga tggccgcaag
    28021 cgagcggtac agacgcacga acgcggccgc gagctcccag gccttactgt cctgcggcag
    28081 cagccaggtg ccggccgcca gacgcgaagt cgtggcggga ctcaggccca gcaatctggc
    28141 aagctgagtc tgccgcaacc cgagctcttc ggatgcgcgc acggccgcct tggagagcac
    28201 ggcggcggga tcttgggcgt tggcagggcg gaggatcata atttcttatg aaatattaaa
    28261 gcaaatatat ttctattgca aataggcccg gcaagtgcgt tgcccgcgcc ccggcctagc
    28321 cgcgcaccgc gccaactgcg gctgctgcca gcagcgtggg tcccagcatg aagtcgacca
    28381 ggcgtggaac ggggcccttc gctgagacca gcacgacgcc ctggtcgcgg acgaaggcga
    28441 gcgcattcct gacctcggtg cgggaagctg aagtcgcggc agagcggggc atagcaggca
    28501 ttatccggca aatagcgatc caccttggcg cgaggggctc gcgacgggcc ggggagcgct
    28561 ggcgtccggc caacggctgc gggaatatct ggcgccggtg tggcgtctaa tcgtcatgga
    28621 acgcatcgtg ctggcagact gacatcgtga gctcacttgc cgccttgcgc aggcgccgtg
    28681 tgtggtttgc actgctggtg ctacccgcac tctcactgcg tgctttgata ccggtcggct
    28741 tcatgcccat ggtgtcggcg ggcggcagga tttccatcga agtctgcccg ggcgtgaccg
    28801 accaggccgc gatccgtggc acgagcgctc gaggagccag ccacgcgcat caccgcggcg
    28861 ctggccagga acatgaaggc gctcgccagg agccctccac tccggcgcat cacgccccgt
    28921 gtctttttgc tgccagcgcg agcggcgcac cggccccgtc cgctcctcaa ctgacagctc
    28981 ctgtcgagca tgtgctgatt gctgatgtcc aaagcatcag cacagttcgt cttcctacga
    29041 tccttcgcac tcaaacatcg cgcggaccac cctcgttctc ctgactccac tgaatgcctg
    29101 tgagctgcgt gcgcgcgatt gatttacggc gtttgcggct acgccgccgc gtcttctccc
    29161 gagccggtga cacacaggtg cctgcacgat cgtggatgta cggggagcgg ctctttggat
    29221 cctggacctg cttggctgca tggcgaagac cgcccggcgc agatcgccgt gcctgttctc
    29281 tcgcttctgt ggttgctcgc tttgctggca tgtccggctg gtgccctagc gcagaccgct
    29341 ggcaccgtcg ggctcgccca gcagcccgcc gccgttgccg acgagcccac caccgtcgca
    29401 tcgcctgcgg agctcacttc cgaaatcggt ccgaggaccg tgacagtcac cgctaacgcg
    29461 gagctgccga tcgacgactc ggtgaccacg accaccagtg tcgatcgcga agacatcgag
    29521 cggacacccg gagcggaccg cagcaacagc ctcggcatga tcacggactt cgtgcccggt
    29581 gcctatgtgg tccacgatca actgcacgtt cgcggcggac accagacgag ctgggcgatc
    29641 gatggggtcg agatccccaa taccaacatc gcgagcaacc tcggcccgca gatcgatccg
    29701 aaggacatcc agtcgctcga ggtggagcgg ggcgcctatg gggccgagct cggcgaccgc
    29761 acgtacggca tcttcaacgt ggcgcccaag accggtttcg atcgcacgaa tcagggcgac
    29821 ctggtcgtca gcggcggcaa tttcggccag accaacgatt atctcagcat cggcagccac
    29881 tcggatgatc tggcttacta cgcgagcatc aacgcaaacc gcagcgacct cggtctgcag
    29941 accccggtgg agcagatcct gcacgactcg gtgtatggct acggcgcatt cacgacgctg
    30001 gttctcgagg ccagcccgca cgatgagctg cgacttgtcg cctccgccag gcaggacgcg
    30061 tacgagatcc cgcagagtcc gggtgaccag atccgggaca tgcagcgcga ggcggatgcc
    30121 ttcacgatcc tgtcatgggc ccgcgctctg ggctcgagcg cagtactcac cagctcactt
    30181 ttctatcatt acaaccgggc cgacctcgat ggggatccgc atgacttccc gatcagcgcc
    30241 acggacagac gatcctcgag ctacgccggc ggacaggaga acctgcacgt gcgcaccgat
    30301 cacaatgatc t
//
LOCUS       DASRHN010000268         6493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_584213, whole genome shotgun sequence.
ACCESSION   DASRHN010000268 DASRHN010000000
VERSION     DASRHN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584213"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1143
                     /gene="ribBA"
                     /locus_tag="VFB37_10710"
     CDS             22..1143
                     /gene="ribBA"
                     /locus_tag="VFB37_10710"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZO22960.1"
                     /translation="MEELLEELRAGRMVVVMDDEDRENEGDLIMPASRVRPEDINFMA
                     RYGRGLICLTLTGERCRQLRLPLMVSDTDRDRRTNFTVSIEAAQGVTTGISAYDRAHT
                     VRTAVQPDAKPEDLRQPGHVFPIMAQPGGVLTRAGHTEAGCDLARLAGFEPAAVIVEI
                     MNDDGTMARRPDLETFARSHQLKIGTIADLIRYRLRNERSVERISQQRVQTEFGEFSL
                     FLYEDHVNRDVHLALTHGPVGDSSVPLVRVHITDTLRDLLGVHGPSRAWTLRAAMERI
                     AQSGSGVIVILRDHESPRDLVAAARALDASDASGSSPAASGAEAARTGGAVLRTYGVG
                     AQILKDLGVRRMRVLSAPKQMHGISAFDLEIESYVGEDS"
     gene            1153..1623
                     /gene="ribH"
                     /locus_tag="VFB37_10715"
     CDS             1153..1623
                     /gene="ribH"
                     /locus_tag="VFB37_10715"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZO22961.1"
                     /translation="MSFPSTLEGEIAARGRKVAIAAARFNDFIVASLLKGATAAWLEH
                     GGAEQDLLVVRVPGAFELPVVARKLALSGHYDAVIALGCVIRGDTPHFDYVAGECARG
                     LQLASLETGVPIVFGVLTVENVEQALQRANLTGVNKGGESMATALEMASLMPRL"
     gene            1620..2066
                     /gene="nusB"
                     /locus_tag="VFB37_10720"
     CDS             1620..2066
                     /gene="nusB"
                     /locus_tag="VFB37_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZO22962.1"
                     /translation="MNQPREANPHFSATRSVARKLALQALYRWQLNDCPWQDLVQEFG
                     DAEDMPRADREYFRELIQGVSGARAALDSQLAGMMDRKPEHLDPVEHAALLIGLYELT
                     SRPEVPWRVSINEAVGLAKRFGATDGHKFVNAVLDRAARALRPDEH"
     gene            2017..3039
                     /gene="thiL"
                     /locus_tag="VFB37_10725"
     CDS             2017..3039
                     /gene="thiL"
                     /locus_tag="VFB37_10725"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZO22963.1"
                     /translation="MPYWTALHARSGQTSTEAMALGEFALIERFFRKCGARRADVRLG
                     VGDDAALLVCPPNCELVAAIDTLVSGVHFLPGSPPASIGHRALAVNLSDLAAMGARPS
                     WALLALTLPAADERWLEEFAAGLAALARAHDVALVGGDTTSGPLCVTVQIMGHVPKSA
                     ALLRSGGHAGDLLFVSGRPGDAAAGLALEQGRLSASEEAAGYLRERFLYPSPRLALGE
                     CLRGHASACIDVSDGLLGDATKLALASNCGLEIDIGRVPVSELLVSAVGEQRARELAL
                     TGGDDYELCFSVPASQVEKLQHNLPPQQWGYTRIGVLREAPGALVTSAGSVIEFAHSG
                     FEHFAS"
     gene            3159..4508
                     /locus_tag="VFB37_10730"
     CDS             3159..4508
                     /locus_tag="VFB37_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO22964.1"
                     /translation="MSLSASKSSLPSKATAPRKIPEKIGKYVVIKEVGRGSTGIVYLS
                     HDGYYGRDVAIKLYNLETGSDEERARISRKMFLSEAHLVGMLQHPHILPIYDAGEEDG
                     RCYIVTEHVHGARTLAAYCRPDNLLRIDDTVEIIFKCAKALHYAHSRGVIHRDIKPSN
                     IMLTQDSDVRIIDFGIALVSDSDISRIEGIAGSPSYMSPEQVQSLELTNRSDLYSLGA
                     VMYELLTGSRPFRAGNLQKLLHQIVYATPPPIHTLRKEIPEELETVVATALQKDPAKR
                     CKSGLDFAAELTRVHQRLREANSRIDRQEQFGVLRRLKFFHEFSHAEIWEVLRASNWQ
                     DYGADEEIVKEGEMDDRFYIIVSGGCVVERHGHRLGTLDTGDCFGEASYVPGAKRTAT
                     IRSASEVTVLKVSSTLLEQVSASCQLRFNRVFLSSLIGRLQSAENTYSGPAPLIIPD"
     gene            complement(4600..5430)
                     /gene="folE2"
                     /locus_tag="VFB37_10735"
     CDS             complement(4600..5430)
                     /gene="folE2"
                     /locus_tag="VFB37_10735"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HZO22965.1"
                     /translation="MTAPVTAKIPRRVFAEVEDVQARADTRQLPINRVGIKDVSHPVR
                     VRDRSAGEQHTIATFNMYVSLPHNFKGTHMSRFVEILHGEREISVESFRTMLTTMTER
                     LEADTGHIEMSFPFFVMKKAPVSGVESLMSYEASLIGERREGRTEMWVRVIVPVTSLC
                     PCSKKIADYGAHNQRSHITIKARLRTHIWIEELIEVAESEASCELYGILKRPDEKYVT
                     ERAYDNPKFVEDMVRDVAVRLNTDDRIAAYVVESENFESIHNHSAYALIEHDKDSTAG
                     "
     gene            complement(5620..>6493)
                     /locus_tag="VFB37_10740"
     CDS             complement(5620..>6493)
                     /locus_tag="VFB37_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO22966.1"
                     /translation="AMREGSGLVEFSRRFPDRYFDVAIAEQHAVTFAAGLATEGFKPI
                     VAIYSTFLQRAYDQLIHDVALQNLPVVFALDRAGLVGSDGATHQGSYDLTFLRCIPNM
                     VIMAPADENECRQMLYTATTLSSPSAVRYPRGSGPGVPLVAEMTALPVGHAQLRREGR
                     SGLAIFAVGALVDSAQKIAERLDATLVNVRFVKPLDEDLLISVAARHRAIVTIEENAT
                     IGGTGSAVAELLASDGLQVPLLQIGIPDRFIEHGSRDSCLAAAGLDHAGLLSSVERWW
                     ALQPQQRIRSAGSG"
BASE COUNT         1094 a         2094 c         2149 g         1156 t
ORIGIN      
        1 gcctcgcccg ggctgaactc catcgaggag ctgctcgagg agctgcgcgc cggccgcatg
       61 gtcgtcgtga tggacgacga ggatcgggag aacgagggcg acctgatcat gcccgcgagc
      121 cgcgtgcgcc cggaggacat caatttcatg gcccgctacg ggcgcggcct catctgcctc
      181 acgctgacgg gcgagcgctg ccgccagctt cgattgccgc tgatggtgag cgataccgat
      241 cgggatcgtc gcacgaactt caccgtctcg atcgaggcgg cgcaaggggt caccacgggc
      301 atctccgcat acgaccgggc gcataccgtg cgcaccgcgg tgcagccgga tgcgaagccc
      361 gaggatctgc gccagcccgg acatgtcttt cccatcatgg cgcagccggg cggcgtgctc
      421 acgcgtgccg gacatacgga ggcgggctgc gatctggcgc gtctcgccgg attcgagccc
      481 gctgccgtca tcgtcgagat catgaatgac gatggaacca tggcgcggcg tccggatctg
      541 gagaccttcg cacgctccca tcagctcaag atcggcacga tcgcagacct gatccgctat
      601 cgcctgcgca atgagcgttc ggtggagcgg atttcgcagc agcgcgtgca gaccgaattc
      661 ggagagtttt cgctgtttct gtacgaggac cacgtgaatc gcgatgttca tctcgcactc
      721 acgcacggtc ccgtaggcga ctcgagcgta cccctggtgc gagtgcatat caccgacacg
      781 ctgcgcgacc tgctgggcgt gcacggtcct tcgcgcgcct ggacgctgcg tgcggcgatg
      841 gagcgcatcg cgcagtccgg gagcggggtg atcgtgatcc tgcgcgatca cgaatctccg
      901 cgggacctcg tggccgcagc tcgagcgctg gatgcgagcg atgcaagcgg ctcgagtcct
      961 gcagcttccg gcgccgaagc ggcacgcacc ggcggggcgg tgctgcgaac ctacggcgtg
     1021 ggcgcgcaga ttctcaagga cctcggcgtg cgccgcatgc gagtcctgtc ggcccccaag
     1081 cagatgcacg gcatttccgc cttcgacctc gaaatcgaga gttacgtcgg cgaagacagt
     1141 tgagtggctc tcatgagctt tccatcgact ctcgagggcg agattgcagc gcgcggccgc
     1201 aaggtcgcca ttgccgcggc ccgcttcaat gatttcatcg tcgcgagcct gctgaaaggc
     1261 gccaccgcgg cctggctgga acacggcggg gcggagcagg atctgctcgt ggtgcgggtt
     1321 cccggagcat tcgagctccc ggtggtcgcc cgcaagctgg cactcagcgg ccactacgac
     1381 gctgtgatcg ccctcggctg cgtcatccgc ggcgacacac cgcatttcga ctatgtggcg
     1441 ggagagtgcg cgcgcgggtt gcagctcgca agtctcgaga ccggcgtccc gatagtcttc
     1501 ggagtgctga ccgtagagaa cgtggagcag gcgctgcaaa gggcgaatct cacgggcgtc
     1561 aacaagggtg gcgaatcgat ggccactgcg ctcgagatgg cctcgttgat gccgcggcta
     1621 tgaaccagcc ccgcgaagcc aatccgcatt tcagcgcgac ccgctccgtc gcacgcaagc
     1681 tcgcactgca agcgttgtac cgctggcagc tcaatgactg cccctggcag gatctcgtgc
     1741 aggagttcgg cgacgcggag gacatgccgc gcgccgatcg cgagtacttt cgggagctca
     1801 tccagggcgt gtccggtgcc cgggcggcgc tcgactccca gctcgccggc atgatggatc
     1861 gcaagccgga gcacctggat cctgtggaac atgccgcact gctgatcggt ctttatgagc
     1921 tgacatcccg acccgaagtg ccctggcgcg tctcgatcaa cgaggccgtc ggcctcgcca
     1981 aacgctttgg tgccacggat gggcacaagt tcgtcaatgc cgtactggac cgcgctgcac
     2041 gcgcgctccg gccagacgag cactgaagcg atggccctcg gcgagttcgc gctcatcgag
     2101 cggttcttcc gcaaatgtgg cgcgagacgc gctgatgtgc gattgggcgt gggggatgac
     2161 gccgcactgc tggtgtgccc gccgaactgc gagctggtcg cggccatcga caccctcgtg
     2221 tcgggtgtgc atttcctgcc gggctcgccg ccagcctcca tcggccatcg ggctctagcg
     2281 gtcaacctca gcgatctggc ggcaatgggg gcgcgcccaa gctgggcgct gcttgccctg
     2341 acgctgccgg ctgcggacga gcgttggctc gaggagttcg ccgcgggtct cgccgctctg
     2401 gcgcgtgctc acgatgttgc tctcgtgggc ggcgatacga cctcgggtcc attgtgtgtc
     2461 accgtgcaga tcatgggtca cgttcccaag tcggcagcgc tgttgcgatc cggcggccat
     2521 gcgggagact tgttgttcgt gtccggtaga ccgggcgatg cggcggcggg gcttgcgctc
     2581 gagcagggtc gtctcagcgc atccgaggaa gcggccggct atcttcgcga gcgcttcctg
     2641 tatccctcgc cccggctcgc acttggtgaa tgcctgcgag gccatgccag cgcgtgcatc
     2701 gatgtgtcgg atggactcct cggggacgct acgaaactcg cgctcgccag caactgcggc
     2761 ctcgagatcg acatcggcag ggttcccgtg tccgagcttc tggtgagcgc agtcggagag
     2821 cagcgggcgc gcgagctcgc gctcacgggc ggggatgact acgagctgtg cttcagcgtc
     2881 ccggcttcgc aggtcgagaa gcttcaacat aatctgccac cccaacagtg gggctacaca
     2941 cgcatcggtg tgctgcggga agccccaggt gccctcgtta cgagcgccgg gagcgtgatt
     3001 gagttcgcgc attccggctt cgaacacttc gcttcctgag ggcccaacgc tccgaagccg
     3061 gaccgctcgc cgtcggccgg gaccctgtgg catgcatcac agtggcgcag cagcgcaata
     3121 cgtatcctgt cgcctgcttt cgttgcggac gcgagtccat gtccctatcg gcatccaaat
     3181 cctcactgcc ttccaaagcg actgcgccgc gcaagattcc cgagaagatc ggcaagtacg
     3241 tggtcatcaa ggaggtcggc cgcggcagca ccggaatcgt gtatctgtca cacgacggct
     3301 attacggccg tgacgtcgca atcaagctct acaacctcga gacgggcagt gatgaggagc
     3361 gcgcgcgcat ttcgcgcaag atgttcctct ctgaggccca cctggtcggc atgctgcagc
     3421 atccgcacat cctgcccatc tacgatgccg gcgaggagga tggtcgctgt tatatcgtga
     3481 ccgagcacgt gcatggggcg cgcacgctcg ccgcttactg ccgaccggac aacctgctgc
     3541 gcatcgacga taccgtcgag atcatcttca agtgcgcgaa ggcattgcat tacgcccact
     3601 cgcgtggcgt gatccatcgt gacatcaagc cttcgaacat catgctcacg caggacagtg
     3661 acgtgcgcat catcgacttc ggcatcgcgc tggtgtcgga ttcggacatc tcgcgcatcg
     3721 agggcatcgc gggcagccct tcatatatgt ctcccgagca ggtgcagagc ctggagctga
     3781 cgaaccgctc ggacctgtat tcgctcggcg ccgtgatgta cgagctgctc accggatcgc
     3841 ggccgttcag ggccggcaat ctgcagaagc tgctgcatca gatcgtttac gccacgccgc
     3901 ctcccatcca tacgctgcgc aaggaaatcc cggaggagct cgagaccgtg gtggcgacgg
     3961 ccctgcagaa ggaccccgcg aagcgttgca agagcggact cgatttcgcc gcggagctga
     4021 cccgggtcca tcagcgcctg cgtgaggcga actcgcgcat cgaccgtcag gagcagttcg
     4081 gcgtgctgcg gcgactgaag ttcttccacg agttctcgca cgcggagatc tgggaagtgc
     4141 tgcgcgcgag caactggcag gactacggcg cggatgagga aatcgtcaag gaaggcgaaa
     4201 tggacgaccg cttctacatc atcgtctccg gcggctgcgt ggtcgagcgc cacgggcacc
     4261 ggctcggcac gctcgatacc ggcgactgtt tcggtgaggc gagctatgtc cccggggcca
     4321 agcgcacggc gaccatccgc tccgcgagcg aggtgaccgt gctgaaggtc agctccacgc
     4381 tgctcgagca ggtgtcggct tcgtgtcagc tgcgcttcaa ccgcgtcttc ctgagctcgc
     4441 tcatcgggcg gctgcagagc gccgagaaca cctacagcgg cccggcgccg ctgatcattc
     4501 ccgactgatc ttcatcccga ccgactggga agcggggttg tgagatgcgc gtgggggaac
     4561 ccccttgcgc gggagttcat ctcgccggcc ggcggtcaat caacccgcgg tgctgtcctt
     4621 gtcgtgctcg atgagcgcgt acgcggaatg gttgtggatg gattcgaagt tctccgactc
     4681 cactacgtat gcggcgatac gatcatccgt attgagccgc accgcgacgt cccgcaccat
     4741 gtcctcgacg aacttcggat tgtcgtaggc gcgctccgtc acgtacttct cgtccggccg
     4801 tttgagaatg ccgtacagct cgcaggaggc ctcgctctcg gcgacctcga tcagctcctc
     4861 gatccagatg tgcgtgcgca gcctcgcctt gatggtgatg tgagagcgct gattgtgcgc
     4921 accgtagtcg gcgatcttct tcgagcacgg gcacaagctc gtgaccggca cgatcacgcg
     4981 cacccacatc tcggtgcgcc cctcgcggcg ctcgccgatc aggctggcct cgtagctcat
     5041 caggctctcg acgccgctca ccggagcctt cttcatgacg aagaacggaa aactcatctc
     5101 gatgtgaccg gtgtctgcct cgaggcgctc ggtcatggtc gtcagcatgg tgcggaagga
     5161 ttcgaccgaa atctcgcgct cgccgtgcag gatctccacg aagcgcgaca tgtgagtgcc
     5221 cttgaagttg tgcggcaggc tcacgtacat gttgaacgtg gcgatcgtgt gttgctcgcc
     5281 ggcggagcgg tcgcggacac gtaccgggtg gctgacgtcc ttgatgccga cacggttgat
     5341 cggcagctgg cgcgtgtcgg cccgggcctg cacgtcctcg acttcggcga agacgcgacg
     5401 tgggatcttg gcagtgacgg gggcagtcat gggctcaagc ctacctcaat ggcactgccg
     5461 gtaatatcta tccggcgtgg gccaactcaa cccccaaccc gagagaggaa ggccggacgc
     5521 ccccgttccc gagcggcccg ccgccggggg tcccttacct cgaagggaac ggccgcgcgg
     5581 ggcggccaaa aggggcggcc agacggaacg ctgttggctt cagccgctgc cggcggagcg
     5641 gatgcgctgc tggggctgca gtgcccacca tcgctcgacg ctcgagagca gaccggcgtg
     5701 atccagccct gcggccgcca ggcagctgtc gcgcgagccg tgctcgatga accggtccgg
     5761 gatgccgatc tgcagtagcg ggacctgcag gccgtcggaa gcgagcagtt ccgcgaccgc
     5821 cgatccggtg ccgccgatgg tggcattctc ctcgatcgtc acgatggcgc ggtgtcgggc
     5881 cgcaaccgag atcagcaggt cctcgtcgag cggtttgacg aatcgcacgt tgacgagcgt
     5941 tgcatcgagc cgctcggcaa tcttctgcgc cgaatccacg agcgcgccca ccgcgaagat
     6001 cgcaagcccg ctgcgtcctt ctcggcgcag ctgcgcgtgg ccgactggca gcgccgtcat
     6061 ctcggcgacg agcggcactc cgggccccga gccgcgcgga tagcgtacgg ccgatggcga
     6121 ggacagcgtg gtcgcggtgt agagcatctg gcggcattcg ttctcatccg ccggggccat
     6181 gatcaccatg ttgggaatgc agcgcaggaa cgtcagatcg taactgccct ggtgcgtggc
     6241 gccgtcgctg ccgaccaggc ccgcccggtc caatgcgaag accacgggca gattctgtag
     6301 cgcgacgtca tggatgagct gatcgtacgc gcgctgcagg aaagtggaat aaatggccac
     6361 gatgggcttg aagccttcgg tggccagccc cgcggcgaac gtgactgcgt gctgctcggc
     6421 gatcgcgacg tcgaaatacc ggtccgggaa gcggcgtgag aactccacca gccccgagcc
     6481 ttcgcgcatg gcc
//
LOCUS       DASRHN010000269         9145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2397665, whole genome shotgun sequence.
ACCESSION   DASRHN010000269 DASRHN010000000
VERSION     DASRHN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397665"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VFB37_10745"
     CDS             complement(<1..953)
                     /locus_tag="VFB37_10745"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZO22967.1"
                     /translation="MKPAVNVAITGAAGQIGYALAFRVASGQMLGSDQPVNLHLLEIS
                     AALPALQGLLMELNDCAFPTLNKVVATDDGRVAFRDCQAALLVGARPRGPGMERKDLL
                     MANAQIFSGQGRALNEVADRNVKVLVVGNPANTNALIARANARDLNPHHFTAMTRLDH
                     NRALSQLAEKTGTHVTAIRRMTIWGNHSSTQYPDISHTLVNGKPATSLVERKWIEENF
                     IPVVQQRGSAIIKARGASSAASAASAAIDHIHDWVHGTAPGDWVSMAVPADGSYGVRE
                     GVIYSYPVTIKGGSYEIVQGLEVDEFSRKRMDATDKELREER"
     gene            complement(1003..1710)
                     /locus_tag="VFB37_10750"
     CDS             complement(1003..1710)
                     /locus_tag="VFB37_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22968.1"
                     /translation="MSGTGHEDDFEAYLRQRSVLPGRPLSSQRLEPPSDLDDLVLKKA
                     RQAIHAREPLPLYRAPRWAVPVALVATVLLTLSILLNVSLTTRRQIPPAVSAQTRSSA
                     STSKPEALQSVAPPSAESAPANGASADAGSPAATYAPSAVTTAPEPAPRANDVAAEAA
                     RQSAAAALSAAPEASRRAAAAAKSRADPQTWLRRIESLRKQGRTAEADAELRRFRQVF
                     PGYPLKAPAADARDPAK"
     gene            complement(1707..2309)
                     /locus_tag="VFB37_10755"
     CDS             complement(1707..2309)
                     /locus_tag="VFB37_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZO22969.1"
                     /translation="MSSDDEDGELMLRYARGDMRAFEILYARNRSGLYRYLARHTHHS
                     EIANDLFQEVWSKVIASRERYEPRAKFRTFLYRIAHNCFIDHCRRAPTRSERGAPVES
                     WESTLAGPDHDRPDLRAEQAELTACYRAALAALPAEQRDVYLLYEESGLSLEEIATIS
                     AVGPETAKSRLRYAVAKLRAALAAREQTPRDAAAIEEPGT"
     gene            2636..2845
                     /locus_tag="VFB37_10760"
     CDS             2636..2845
                     /locus_tag="VFB37_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22970.1"
                     /translation="MSTVNNGLKALACGFGALAITMVLSWSFVESTTAAPSVTLDAPA
                     THMAKLTIQPRHIWFGQSQPAVLVD"
     gene            2886..3302
                     /locus_tag="VFB37_10765"
     CDS             2886..3302
                     /locus_tag="VFB37_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO22971.1"
                     /translation="MDPQWDDSVPIYRQLRDRVVAMILEGALKEGDPLPSVRSVAAEF
                     RLNPLTVLKGYQQLVDEQLVEKRRGLGMFVSPGASKALMKDERQRFLEEEWPKVYATI
                     QRLGLSPADLLKAGATAAPSAPWPTTPGQDKDDSSN"
     gene            3286..4155
                     /locus_tag="VFB37_10770"
     CDS             3286..4155
                     /locus_tag="VFB37_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO22972.1"
                     /translation="MTPVIEARDLRKHYGANRAVDSVNFSVAPGRIVGLIGPNGAGKT
                     TVLKAILGLTRFDGTLHVLGRDPDSQRDDLMQDVCFIADVAVLPKWLRVRNALDFVAG
                     VHPRFDRARAEEFLRKTDIRLSSRVRELSKGMVTQLHLALILAIDARLLVLDEPTLGL
                     DLLFRRQFYDTLVNDYFDKERTILLTTHQVEEIEHLLTDVIFISRGQIILDSAVEDLS
                     TRYTQVTVAADKAAALRELKPFYEREVFGRLALFFEGRSAAELTAAAGPLSELRTPSI
                     ADLFVARMQGTAS"
     gene            4152..5126
                     /locus_tag="VFB37_10775"
     CDS             4152..5126
                     /locus_tag="VFB37_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO22973.1"
                     /translation="MSTADTNLSSSERPVADAPHASGVAGRNRALSTLRILIQREFWE
                     HRALWMAPVMTSVLLIACAFPAHVDFPDFSRAASKGPLHELAWYAAMQWALTGVQFLI
                     AGIVLNFYLLDCLYAERKDRSILFWKSLPASDGATVTSKLLVGEVLVPLGIFVLSIAT
                     NLLFSLVWAGRAALGYLPDVAVWDTMSWVKVEVAVLLTLVICILWYAPLCAYLLLVSA
                     WARRSAFLWATLPPVLAVLLERVAFGTHYVLNLIGYRTAGIFHYVDMQKIVQATVLSG
                     PHGKPFSLAAISLELSTAFGNIDLWLGVLVAVALTYAAARIRRYRDDT"
     gene            5255..6388
                     /locus_tag="VFB37_10780"
     CDS             5255..6388
                     /locus_tag="VFB37_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22974.1"
                     /translation="MSAFLEVRRTGESLEVDLTGEWRALSLMAIDAALAALDVAAARR
                     VVIGTDALLALDLSGAWRLNELMRSLRAGGIEVSFRGAPPDQLRLVASARGELPSPPH
                     DPSGEEDLPFPEIGLVEIGIKSVRLVRDLLATLAYVGRVGVTAWRVLRSPRRLRPISI
                     VRHIYDTGITAMPITALIAFLISVIVAYMSAQQLRRFGAEIFVVDLVTIGVLREMGVL
                     LTSIIVAGRSGSAFAAEIGSMQLNEEVDALHAMGVEPFEVLVLPRLLALVIALPLLTV
                     VADIIGLMGGALLCRLLLDIPLSQFVTRVNEAIAPTTFWVGLIKAPVFALLIGMAGTY
                     RGMQVRDSSRELGRLTTVAVVQAIFLVILADALFAVFFMQLDI"
     gene            6403..7206
                     /locus_tag="VFB37_10785"
     CDS             6403..7206
                     /locus_tag="VFB37_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO22975.1"
                     /translation="MPESVRQSAPQEAPRVVLEIRGLVNRFGSQVVHEGLDMDVYEDE
                     IFGIVGGSGTGKSVLLRSILGLQRPQAGTIRIAGLDITRMHAAQLQAIKARYGVTFQQ
                     GALFSALNVLQNVQVPMLEHLRLPATERDELAMLKVRLVGLPADAALKYPAQLSGGMV
                     KRAALARALALDPSLLFLDEPTSGLDPISAAGFDELILYLQKELRLTVLMITHDLDTI
                     FRTCNRVGVIVDRHMEVDTLERIVAHPNPWIQAYFHGARARARTVEHGA"
     gene            7163..8131
                     /locus_tag="VFB37_10790"
     CDS             7163..8131
                     /locus_tag="VFB37_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO22976.1"
                     /translation="MERAHGRARSNMEREANYAAVGAFVLLIALVAALFVYWYSDTRE
                     HRVYQRYEIYFSGSVSGLERGSAVRYLGVGVGRVLEMHIDPRDPGRVQVVVGIDSATP
                     ISDKTLAQLQMQGVTGLLYIDLQQVGPGTTLPEPVPGIRYPVIRSTQSRFDLFLASLP
                     DVLAAVGDVVDRAGRVLNDQNIAAISRALQNVDKASAGLPATLREVNALVSDLRGAAT
                     ELQASARGAHEVLNQAGPEVVAAVQRVHDVADNLSKASSQLERLIQDNRQDLRSFTRD
                     GLPELEQFVREGRAATQEIRELSSSLRENPSQLIYQPPHQGVEIPR"
     gene            8128..8793
                     /locus_tag="VFB37_10795"
     CDS             8128..8793
                     /locus_tag="VFB37_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HZO22977.1"
                     /translation="MKHPVHLGKLVAALAGVVALLGVAACAGGFHSDAPATQVYVLRA
                     GAGAQTQLPRASAPEFSIHVTRPLAGPGLGSDHIMLVQSDHRMSYYAASRWPASVPEV
                     VEALAVETLRTSGNWSAVQDSAGGFSSDYLLQIVIQRFEADYSSHPDAPEVHVVLDCT
                     LGRGVGRELVGTFVAEGAATASVNHLTEVISAFEAAASQALRSMALQAAQKSEAPVAS
                     RNR"
     gene            complement(8754..>9145)
                     /locus_tag="VFB37_10800"
     CDS             complement(8754..>9145)
                     /locus_tag="VFB37_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZO22978.1"
                     /translation="GGIPNQPVASELVAASDGAIHVRWYRTHGEQFHTKLAMVYGAQR
                     LWLTVGSANLTRRALLDYDLEANVAVDLERTAPLAMQVLDYFDTLWSNRAPLGIEYTA
                     DFGFYSDPSQLHYWLYRFLEATGASLF"
BASE COUNT         1494 a         2923 c         3010 g         1718 t
ORIGIN      
        1 cgctcttccc ggagctcctt gtcggtggcg tccatccgct tgcggctgaa ctcatccacc
       61 tcgaggccct gcacgatctc gtaactgccg cccttgatcg tgacggggta ggagtagatg
      121 acaccctccc ggactccgta actgccgtcg gccggcacgg ccatgctcac ccagtcgccg
      181 ggtgcggtgc catgcaccca atcatgaata tggtcgatcg ctgcggaagc ggccgaggcg
      241 gcggacgatg ccccgcgagc cttgatgatg gccgagccgc gctgctgtac gacaggtatg
      301 aaattctcct cgatccactt gcgctccacg agcgatgtcg ccggtttgcc gttcacgagc
      361 gtgtggctga tgtccggata ctgagtcgag gagtgattgc cccagatcgt catgcgacgg
      421 attgccgtga cgtgcgtgcc ggtcttctcg gcgagctggc tgagggcacg gttgtgatcc
      481 aggcgtgtca tggccgtgaa atgatgtgga ttcagatcgc gcgcgttggc ccgggcgatc
      541 agcgcattcg tgttggccgg gttgccgacg accagcactt tcacgttgcg atcagccacc
      601 tcgttcaggg cccgaccctg gccagagaag atctgcgcgt tggccatcag caggtccttg
      661 cgctccattc ccgggccgcg tggccgcgct ccgacgagca gcgcggcctg acaatcgcga
      721 aaagcgaccc ggccatcgtc cgtggcgacc accttgttca aggttggaaa agcacagtcg
      781 ttgagctcca tcagcagacc ctgcagcgcc gggagcgcgg cgctgatctc cagcagatgg
      841 agattgaccg gctgatcgga cccgagcatc tggcccgagg cgacgcggaa ggcgagcgca
      901 taaccgatct ggccggcggc gcccgtgatc gcaacattga cggctggctt catggcggtc
      961 tttctcggcg gagcgggccg cggattctac tgcaaccgga cgctatttcg ctggatcgcg
     1021 cgcatcggcc gcgggcgctt tgagcggata gccggggaat acctggcgaa aacggcgcag
     1081 ctcggcgtcg gcctcggcag tcctgccctg cttgcgcaga ctttcgatcc gccgcagcca
     1141 ggtctgtgga tccgccctgg atttcgcggc ggcggccgca cggcgcgaag cttcgggcgc
     1201 ggcggaaagc gctgccgcgg cggactggcg cgcagcctcg gccgccacat cattggcccg
     1261 gggggcgggc tcaggggctg tcgtgactgc agagggcgca tacgtcgcgg cgggtgagcc
     1321 tgcatcggcc gaagcgccat tggcgggcgc ggattcagcg gatggaggcg ccacgctctg
     1381 gagcgcctcg ggcttcgatg tggaggcgga gctacgcgtc tgagcgctca ccgccggcgg
     1441 gatttgccgg cgggtcgtca ggctgacatt cagcagaatc gagagcgtca gcagtacggt
     1501 cgccacgagc gccaccggca ccgcccatcg gggtgcccga tacaacggca acggctcgcg
     1561 cgcatgaatg gcctggcgcg ccttcttcag cacgaggtca tcgagatcgc tcggcggctc
     1621 gagacgttgg gaggaaaggg gccggcccgg cagcacggaa cgctggcgca ggtaagcctc
     1681 gaagtcgtcc tcatgtccgg taccgctcat gtaccgggct cctcgatcgc ggccgcgtcg
     1741 cgcggcgttt gctcgcgtgc cgccagggca gctcgcaact tcgcgaccgc atagcgcaac
     1801 cggctcttgg cggtctccgg tcccactgcg ctgatcgtcg cgatttcctc cagcgacaga
     1861 ccggactcct cgtacagcag atacacatcg cgctgctcgg ccggcaacgc ggcgagcgcg
     1921 gcccgatagc acgcggtcaa ctcggcctgt tccgcccgca gatcgggtcg atcgtgatcg
     1981 ggcccggcca gcgtgctttc ccaactctcg acaggcgcac cgcgctcgct tcgagttgga
     2041 gcgcgccggc agtgatcgat gaagcagttg tgcgcgatgc gatagagaaa cgtgcggaat
     2101 ttcgcgcggg gctcgtaccg ctcgcggctg gcgatcacct tgctccacac ctcctggaac
     2161 agatcgttgg caatctcgct gtggtgagtg tgccgggcga gatagcgata aagcccgctg
     2221 cgattgcgtg cgtagagaat ctcgaaggcg cgcatgtcac cgcgcgcata acgcagcatc
     2281 aactcgccgt cttcgtcgtc gctgctcact gcttcaactg tacgcgttgt cgtgcaccgg
     2341 gccaaaagcg cggcgctcca accttttcgg ctgcatcgct ctccaagtgg gcgcaaggag
     2401 ccggcacgca gcaaggaggt ctcgcccccc gccctccagg ggtcattggc cggctcgggg
     2461 tccgggggtc ccttcagggc ctcaggcagc ccccgcgggg ccccttcccg ggcccttcgg
     2521 ggatccagaa aatatttggc gatatggctt gacactagtg tagtgatgta gttatataca
     2581 acaccagaga ggggaccgca acgggcgatc cccaggtgaa acgaaggaga agacgatgag
     2641 cactgtaaac aatggtctga aggccctggc atgcggcttt ggcgccctgg cgatcacgat
     2701 ggtgctgagc tggagctttg tcgagtccac gacggcggcc ccgagcgtga cactggacgc
     2761 gcctgcgacc cacatggcta agctgacgat tcagcctcgg cacatctggt tcgggcagtc
     2821 ccagccggcc gttttggtag attagttggc tatccttccg tcacggcatg ggctccggag
     2881 tgttaatgga tccgcagtgg gacgacagcg tgccgatcta tcggcaactg cgggaccgag
     2941 tggtggcgat gattctggaa ggcgccctca aagaaggcga tccgctaccc tcggtccgca
     3001 gcgtcgctgc cgagttccgg ctgaatccac tcacggtgct caaggggtac cagcagctcg
     3061 tcgatgagca gctggtcgag aagcggcgcg gcctcgggat gttcgtaagc cccggggcca
     3121 gcaaggcgct gatgaaggac gagcgccagc gctttctcga agaggaatgg cccaaggttt
     3181 acgccaccat ccagcggctg ggactctcac cggccgacct gctcaaagca ggggccacgg
     3241 ccgctccgag cgcgccctgg cccacgacgc ccggacagga caaagatgac tccagtaatt
     3301 gaagcgcgcg atctgaggaa gcactacgga gccaaccggg cggtggactc ggtgaacttc
     3361 agcgtggcgc cggggcgcat cgtggggctg atcggtccca atggcgcagg caagaccacg
     3421 gtgctgaagg caatactcgg cctcacgcgc tttgacggta cgctacacgt tctggggcgc
     3481 gatcccgatt cacagcgtga cgatctcatg caggacgtct gcttcattgc cgacgtcgcg
     3541 gtactgccca agtggctgcg cgtgcgcaac gcactcgatt tcgtcgccgg agtacacccg
     3601 cggttcgacc gcgcgcgtgc ggaagaattt ctgcgcaaaa ccgacatccg cctgagcagc
     3661 cgcgtgcgcg agctgtccaa gggcatggtc acgcagctgc atctcgccct gattctggcg
     3721 atcgatgcgc ggctgctggt gctcgatgag cccacactcg ggctcgatct gctgttccgg
     3781 cgccagttct atgacacgct ggtcaatgac tacttcgaca aggagcgcac gattctcctc
     3841 acgacgcacc aggtcgagga gatcgagcat ctgctcacgg atgtgatctt catcagtcgc
     3901 ggccagatca ttctcgacag cgcggtggag gatctgtcca cgcgctacac gcaggtcacg
     3961 gttgcggcgg acaaggccgc cgcgctgcga gagctcaagc cgttctacga gcgtgaggtt
     4021 ttcggacggc tcgccttgtt cttcgaaggg cgctccgcgg cggagctgac tgcagcggcc
     4081 ggtccgctct cggagttgcg cacaccgtcg atagcggacc tgtttgtcgc caggatgcag
     4141 gggacggctt catgagcacc gcagacacga atctctccag ctccgagcgg cccgtcgcgg
     4201 atgcgccgca cgcatccggt gtcgctggtc gcaatcgcgc tctgagtacg ctgcggatcc
     4261 tgattcagcg ggagttctgg gagcatcgtg cgctctggat ggcgcctgtg atgacctccg
     4321 ttctgctgat cgcctgcgcc tttccggcgc acgtggactt ccctgacttc tcgagggccg
     4381 cctcgaaagg ccccctccac gagctggcct ggtacgcggc gatgcagtgg gccctgacgg
     4441 gcgtacagtt cctgatcgcc ggcatcgtgc tcaacttcta tctgctcgac tgcctgtatg
     4501 ccgagcgcaa ggatcgcagc atccttttct ggaagtcact gcccgcctcc gatggcgcca
     4561 cggtcacttc caagctgctg gtcggcgaag tcctcgtccc gctcggcatc ttcgtgcttt
     4621 ccatcgccac caacctcctg ttctcgctgg tgtgggcggg gcgcgcggcc ctgggctatc
     4681 tgccggacgt ggctgtctgg gacacgatgt cctgggtcaa ggtcgaagtc gctgtgctgc
     4741 tcacgctcgt catctgcatc ctgtggtacg cccccctctg cgcctacctg ctgctcgttt
     4801 cggcctgggc gcgccgcagc gctttcctct gggcgacgct gcccccggtg ctcgcagtcc
     4861 tgctcgagcg cgtggccttc ggcacccact atgtgttgaa tctcatcggc tatcgcacgg
     4921 ccggaatatt ccactatgtg gacatgcaga agatcgttca ggcgacggtg ctgtccggcc
     4981 cgcatggcaa gcctttctcc ctggcggcca tcagcctcga actgtccact gccttcggca
     5041 acatcgacct gtggctcggg gtgctcgtgg ccgtcgcgct cacctatgcg gccgcccgca
     5101 tccggcgcta tcgagacgat acttagcggt cgcgatagat cgcgcttatc attccaattc
     5161 aaactgctca ttggtatcac gctgcgcgtg ggccgagcag tgagaaggtc ggggagcagc
     5221 tgcaggcggt gaaaggccca cggagcagtc cttcttgagc gctttcctcg aagtgcggcg
     5281 caccggggag agtctcgagg tcgatctgac cggggaatgg cgcgcgctga gtctgatggc
     5341 gattgacgct gcgctcgcag cgctggatgt cgccgccgcg cggcgggtag tgattggtac
     5401 cgatgcgctg cttgcgctgg atctctccgg tgcctggcgc ctcaatgagc tgatgcgaag
     5461 cctcagggcg ggcgggatcg aagtctcctt tcgaggagcg ccccccgacc agctccgact
     5521 ggtggcatcg gccaggggag agctcccctc ccctccacac gatccgtccg gcgaagagga
     5581 tctgcctttt ccggagatcg gtctcgtcga gatcggcatc aagtccgtgc gcctcgtgcg
     5641 ggacttgctg gcgacactgg cgtacgtggg ccgcgttggg gtcacggcgt ggcgagtact
     5701 gcgcagcccg aggcgcttgc gacccatctc catcgtgcgc cacatctacg atacgggcat
     5761 caccgcgatg ccgatcacag cgctgatcgc gttcctgatc agcgtgatcg tggcctacat
     5821 gagcgcccag cagctgcgcc gattcggggc cgagatcttc gtcgtggacc tggtgaccat
     5881 cggtgtgctc cgagagatgg gtgtgctgct gacctcgatc atcgtcgccg gtcgctcggg
     5941 cagcgcgttt gccgcggaga tcggctcgat gcagctcaat gaagaggtgg acgccctgca
     6001 cgcgatgggc gtcgagcctt tcgaggtgct ggtgctgccg cggctgctcg ctctggtgat
     6061 cgccctgcct cttctgaccg tggtagcgga tatcatcggc ctcatgggcg gagctttgct
     6121 gtgccggttg ctgcttgaca tccccctcag tcaattcgtg actcgcgtca acgaggcgat
     6181 cgccccgacc accttctggg tgggactcat caaggcgccg gtgtttgccc tgctgatcgg
     6241 catggcagga acctatcggg gcatgcaggt ccgcgactcc tcgcgcgagc tgggccgcct
     6301 gaccaccgtg gcggtcgttc aggccatctt cctggtcatc ctggccgatg cgctgttcgc
     6361 ggtgttcttc atgcaactcg atatctgaag gcaccgcccg tgatgcccga atctgttcgg
     6421 cagagcgcgc cgcaggaggc gcctcgggtt gtcctcgaga tacgcggcct cgtcaaccgg
     6481 ttcggctctc aagtggtgca cgagggcctc gacatggatg tgtacgagga cgagatattc
     6541 ggaatcgtgg gcggatccgg gaccggcaag tccgtgctgc tgcggagcat cctcgggctg
     6601 cagcgcccgc aggcgggcac gatccgcatc gccgggctcg acatcacccg catgcacgcc
     6661 gcacaactgc aggccatcaa ggcccggtac ggtgtgacct tccagcaggg agcgttgttt
     6721 tccgctctca acgtgctgca gaacgtccag gtcccgatgc tcgagcacct gcggctgccg
     6781 gccaccgagc gtgacgagct ggccatgctg aaagtgcgcc tcgtggggct gccagccgat
     6841 gcggcactca aatatcccgc gcagctctcc ggaggcatgg tcaagcgcgc cgcgctcgcg
     6901 cgggcgctgg ccctggaccc gagccttctg tttctggacg agccgacttc gggtctggat
     6961 ccgatcagcg ctgcaggttt cgacgagctg attctgtatc tgcagaaaga gctgcgcctg
     7021 acggttctca tgatcacaca tgatctggac acgatcttcc gtacctgcaa tcgtgtaggg
     7081 gtgatcgtcg atcggcatat ggaagtcgac accctcgagc gcatcgtcgc gcaccccaac
     7141 ccgtggattc aggcgtactt tcatggagcg cgcgcacggg cgcgcacggt cgaacatgga
     7201 gcgtgaagcc aactacgccg cagtgggcgc gttcgtgctc ctgatcgcgc tggtcgcggc
     7261 gctgttcgtc tactggtatt cggatacgcg cgagcatcga gtctatcagc gctacgagat
     7321 ctacttctct ggcagtgtca gcggcctcga gcgcggatcg gccgtgcgat acctgggtgt
     7381 cggcgtcgga cgcgtcctgg agatgcatat agatcctcgc gatccgggtc gtgtgcaggt
     7441 agtcgtcggg attgattcgg cgacgccgat ctccgacaag acactggcac agctgcagat
     7501 gcagggcgtg accgggctgc tctacatcga cctgcaacag gtgggcccgg gcacgacgct
     7561 gcccgagccg gttccgggca tccgctatcc ggtcatccgc tcgacccagt cacgcttcga
     7621 tctgtttctg gcgagcctgc ccgacgttct ggccgccgtg ggagatgtgg ttgaccgtgc
     7681 cgggcgagtg ttgaatgacc agaacatcgc ggcgatttcg cgcgccctgc agaacgttga
     7741 caaagcgagc gcgggcctgc ccgccacgct gcgcgaggtc aatgcgctgg tcagcgattt
     7801 gcgtggcgcc gccaccgagc tgcaggcctc tgctcgaggc gctcatgagg tgctgaacca
     7861 ggccgggccg gaagtggtag cggccgttca gcgtgtgcac gacgtcgcgg ataacctttc
     7921 gaaagccagc agtcagctcg agcgcctcat ccaggacaac cgccaggatc tgcgctcctt
     7981 cacgcgcgat ggcctgcccg agctcgagca gttcgtgcgc gaagggcggg ccgcgacgca
     8041 ggagatccgc gagctctcga gcagcctgcg tgagaatcca tcgcagctca tctatcagcc
     8101 gccccaccag ggcgtcgaga tcccgagatg aagcaccctg ttcacctggg aaagctggtc
     8161 gccgccttgg caggtgtggt tgctctgttg ggggtcgcag cttgcgcggg agggtttcac
     8221 agtgacgcgc ccgctacaca ggtgtacgtc ctgagagccg gagccggtgc ccagacgcag
     8281 ctgccgcgcg ccagcgcgcc cgagttctcc atacatgtca cgcgccccct ggcaggtccg
     8341 gggttgggct cggatcacat catgctggtg cagtccgatc atcggatgag ttattacgcc
     8401 gcaagccgct ggcctgccag cgtgcccgag gtggtcgagg cgctggccgt ggagacctta
     8461 cggacctccg gcaactggag cgcagttcag gactccgcgg gcgggttctc ctccgattat
     8521 ctgctgcaga tcgtgatcca gcgtttcgag gcggactaca gctcgcatcc ggatgccccc
     8581 gaagtgcacg tcgtgctcga ttgcacgctg ggcaggggcg tcggacggga gctcgttggc
     8641 accttcgtgg cagagggtgc cgccaccgcc tcggtgaacc atctcaccga ggtcatctcc
     8701 gcattcgagg ccgccgccag ccaggcactg cgttcgatgg cactgcaggc cgctcagaag
     8761 agcgaggcgc cggttgcttc gaggaatcga tagagccagt agtgaagctg cgaggggtcg
     8821 gagtagaagc cgaagtcggc cgtgtactcg atcccgagcg gggcgcggtt gctccacaga
     8881 gtgtcgaagt aatcgagcac ctgcatcgca aggggcgcgg tgcgctccag atcgaccgcc
     8941 acgttcgctt cgaggtcgta gtccagcaga gcgcgccggg tcaggttcgc cgagcccacc
     9001 gtgagccaga gccgctgcgc gccgtacacc atggcgagct tggtgtgaaa ttgctcgcca
     9061 tgggtgcgat accagcgcac gtggatggcc ccatcactcg ccgccaccag ctcgctggca
     9121 accggttgat taggtattcc gcccc
//
LOCUS       DASRHN010000270         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_917297, whole genome shotgun sequence.
ACCESSION   DASRHN010000270 DASRHN010000000
VERSION     DASRHN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917297"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFB37_10805"
     CDS             complement(<1..668)
                     /locus_tag="VFB37_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZO22979.1"
                     /translation="MNALRAQHKTPVALLLSMLAVPFVAHASSDDYLKTYSVGNHPEV
                     RVQTNDSSVRVVTSDTNQVEFRVHSEGVRAALGFGGQVKVDSHQSGNRVELTVTATPS
                     FTIGIDIRRLETEVHMPKDADLLIDTHDGAIELSAINGNITIHTRDGSIKATELSGRI
                     DLDTSDGGISAASLKGEARLHSGDGAIEADSMDGKLDASTSDGSIHVEGRFDALDVKS
                     GDGTV"
     gene            941..1474
                     /locus_tag="VFB37_10810"
     CDS             941..1474
                     /locus_tag="VFB37_10810"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HZO22980.1"
                     /translation="MKSWIVLLRGINVLGNNMLPMKELADTLERAGFRSVRTYLQSGN
                     VVLQSARGTARTLSKRISNLILERFGFLPKVMVISAAELADAVRGNPFPGADKNHKAL
                     HLYFLAEHPRQPDLAALARLDVGREAFALKGCVFYLYTPDGFPDSILRSRIERCLGVA
                     ATARNWRTANELLKMVR"
     gene            complement(1573..2277)
                     /locus_tag="VFB37_10815"
     CDS             complement(1573..2277)
                     /locus_tag="VFB37_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO22981.1"
                     /translation="MRALVVDDEPPARREMRRLLAQFPWLEIVGEAGGASEAQAQIEN
                     LCPDLLFLDIQMPGGSGFDLLARLEHVPRVIFTTAHDEHAVRAFDVNALDYLLKPIDP
                     RRLEAALAKIKSADSPAPTEARPALEQLFVRDGTRCWFVPLREVQLLTSEGNYVRLHW
                     GRIRPLLARSLAAVEQRLDPTLFFRANRSQIVNLRFIESIDAGLNGRLHAQLRDGPEV
                     EISRRQARFFKELTSI"
     gene            complement(2274..3269)
                     /locus_tag="VFB37_10820"
     CDS             complement(2274..3269)
                     /locus_tag="VFB37_10820"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZO22982.1"
                     /translation="MISFLHLPWLRAALEIFLLNGIGLLLSDRLHSYIRHRNWSPSPL
                     RPLVGRIIVTSFVLATPLGLLTPFTSVRLLQDPATLPDSVPTFLLRLDPGLTVALNWI
                     NWAAVFALWLIIYFVILSMRRRRFAELRQSELARALQQAELRLLKSQLNPHFLFNSLN
                     TVRSLIADDPQRAQNAVTRLANTLRYTLGAGQDEKVSLARELEIVADYLEIESIRFET
                     RLSIRYQVSAEAAGVQIPVMLLQTVVENAIKHGIAELPQGGVLEIRGIVQDGMLQLSV
                     MNSRPPVILPKAEPGTGLRNAAERLRLLFGPTARIELDLSGAEVAVTRISIPAQQ"
     gene            complement(3456..>4119)
                     /locus_tag="VFB37_10825"
     CDS             complement(3456..>4119)
                     /locus_tag="VFB37_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO22983.1"
                     /translation="RGAEWTIVGAFDGGGDRHESEMLSGVEVLQSTFQRNTFQSVTVQ
                     LDSAASFADFRASLESNPALGIEVMRESDYYLHQSRAFAQLLTFIAYLVGSIMAIGAV
                     FGALNTMYAAVSARAVEIATLRVLGFEPGSIVTSVFTEALVLAALGGALGGILAWLLF
                     SGHAISTNGGGLTQLSVPIVVNARLVAVGILWACIIGMVGASFPALRAIRAPLACALK
                     AT"
BASE COUNT          795 a         1254 c         1331 g          739 t
ORIGIN      
        1 acggttccgt ctccggactt gacgtccagg gcgtcaaatc gaccttcgac gtgaatcgat
       61 ccatcactgg tggaggcatc gagctttccg tccatagaat ccgcttcgat cgcaccgtca
      121 ccgctatgca ggcgcgcttc acctttcaga cttgctgcgc tgatcccgcc gtccgaggtg
      181 tccaggtcga ttcgtcccga gagctctgta gccttgatcg agccgtcccg ggtgtgaatg
      241 gtgatgttgc cgttgatggc cgaaagctcg atcgccccat cgtgagtgtc gatcagcagg
      301 tcggcgtcct tgggcatgtg aacttcggtt tcgagtcttc tgatgtcaat gcctatcgtg
      361 aacgagggag tggccgtcac ggtcagctcc actcgattac cgctctggtg ggaatcgacc
      421 ttcacctggc cgccaaatcc gagcgcagcg cgtacccctt ccgagtggac tcggaactct
      481 acttgattgg tgtccgaagt cacgacgcgc acactgctgt cattggtctg gacgcgcact
      541 tcgggatggt tgccgaccga atacgttttg aggtagtcat ccgagcttgc gtgggcgacg
      601 aatgggactg ccagcatcga caggaggaga gcgactgggg ttttgtgctg ggcgcgaaga
      661 gcgttcatgg ggttgccgtt gcaaaagagt ggcaggatgg tatcagggct tgtgggctgc
      721 tgcgcacggc ttggcgcgct cttggggcgg atcgccccgc tttccgggaa gcgcaccgct
      781 tctgaaacgc gtggctctgg aaggccctga tgaggtaccg ggaaccgtgc gatggcggcc
      841 catgatgtgg gccgaatgcg accgtcaaat caatgccttc catccatcct ggcttcgtca
      901 aggtgagcag ctgcacggct gcaccggaga agttgacgag atgaagagct ggatcgtgct
      961 gttgcgcgga atcaatgtac tgggcaacaa catgctgccg atgaaggagc tcgccgacac
     1021 gctcgagcgc gcgggctttc gatcggtgcg cacctacctc cagagcggga acgtcgtcct
     1081 gcaaagtgcc agggggaccg cgcgaacgct gtcgaagcgg atctcgaacc tcattctcga
     1141 gcgctttggc ttcctgccga aggtgatggt gatcagcgcc gcggagctcg cggacgcggt
     1201 tcgcggcaac cccttcccgg gcgcggataa aaatcataag gcgctgcatc tgtatttcct
     1261 cgcggagcat ccgagacaac cggacctcgc cgcgctggcg cgcctggatg tggggcggga
     1321 agcgttcgct ctcaagggtt gcgtgtttta cctctatacg cctgacggat ttcccgactc
     1381 gatcctgcgc tcgcgaatcg agcgctgcct gggggtcgcc gcgaccgccc gcaactggcg
     1441 gaccgccaac gagctgctca aaatggtgcg gtgatgctcc tcggccgttc gatcctcgcg
     1501 ggcggttgca agcctgcccg gcagcgactg cgcttcatcc gacatgacat tgaaggggcg
     1561 cgccgggccc gctcagatgc tggtgagctc cttgaagaaa cgagcctgcc gtcgggagat
     1621 ctccacctcg gggccatcgc gcagttgcgc gtgcaggcgc ccgttgaggc ccgcatcgat
     1681 gctctcgatg aaacggagat tgacgatctg gctgcggttc gcacggaaga acagggttgg
     1741 gtcgagtcgc tgctcgaccg cagcgagact gcgcgccagc agtggacgga tccttcccca
     1801 gtgcaggcgc acgtagttgc cttccgaggt cagcaattgc acttcgcgca ggggtacgaa
     1861 ccagcagcgt gtcccgtccc gaacgaacag ctgctcgagc gccggccgag cctctgtggg
     1921 agccggtgaa tctgcactct tgatcttcgc cagcgcagcc tccaagcggc gcggatctat
     1981 gggcttcagc aggtaatcca gagcgttgac gtcgaatgcc cgcaccgcgt gctcatcatg
     2041 ggcggtggtg aagatgaccc gaggaacgtg ctccaggcgc gcaaggagat cgaagcccga
     2101 gccgccgggc atctgaatgt cgagaaacaa caggtcgggg caaagattct cgatctgcgc
     2161 ttgcgcctcg gaggccccgc ccgcttcccc cacgatctcg agccacggaa actgcgccag
     2221 caggcgccgc atctcgcgtc gggccggcgg ctcatcatcg acaacgaggg ctctcattgc
     2281 tgcgccggga tgctgattcg ggtcacggcg acctcggctc ctgacagatc cagctcgata
     2341 cgggcggtgg ggccgaagag cagtctcagc cgctccgccg cattgcgcaa gccagtgccg
     2401 ggctcggcct tgggcaagat aacggggggt cgcgaattca tgactgagag ctgaagcatg
     2461 ccgtcctgca cgatgccgcg gatctccagc actcctccct gcggcaactc cgcgatgccg
     2521 tgcttgatgg cgttctcaac gaccgtttgc agcagcatca cggggatctg cactccggcg
     2581 gcttcggcgc tcacctggta tcgaatactc aggcgtgtct cgaaacggat ggattcgatc
     2641 tcgaggtagt ccgcgacgat ttccagctcc cgagccagcg acaccttctc gtcctgaccg
     2701 gctcccaatg tatagcgcag cgtgttggcg agtcgagtca cggcgttctg ggcgcgctgc
     2761 ggatcatccg caatcagaga acgcacggta ttgagcgagt tgaacaggaa gtgtggattg
     2821 agctgcgatt tcagcaggcg cagctctgcc tgctgcagag cgcgtgcgag ctcggactgg
     2881 cgcaattccg caaagcgccg ccgccgcatc gaaagaatga cgaagtagat gatcagccag
     2941 agcgcgaata ccgccgccca gttgatccag ttcaacgcga cggtgagccc ggggtcgagg
     3001 cggagcagga aggtgggaac cgagtccggc agcgtcgccg ggtcctgcag caaccgcaca
     3061 gacgtaaacg gtgtgagcag cccaagcggg gtcgccagga cgaagcttgt cacgatgatc
     3121 cgtccgacca gcggccgcag cggcgaggga ctccagttgc gatgcctgat ataggagtgc
     3181 agccgatcgg aaagcagcaa gcctatcccg ttcagcaaga aaatctcgag cgcggcgcgc
     3241 agccagggca ggtgcaggaa cgagatcacc gcgaccagct gtgctgcggt atgcgctccc
     3301 cacccgatca gctgacaagc ccagtagggc ctgcgggatg aagaggcctt ccgggatgac
     3361 gagccggaaa taatcgagga gtcagctggc aaccgattca taaccgattc atacacgtca
     3421 acggggccgg agactgccgg ggtgtggcat caaggctacg tcgccttcaa cgcgcaggcc
     3481 agaggtgcgc ggatggctcg caaggcggga aaagacgctc ccaccattcc gatgatgcac
     3541 gcccagagga tcccgacggc caccagtctc gcattgacga cgatcggcac ggacagctga
     3601 gtgagcccgc cgccattcgt gctgatggcg tgaccggaga agagcagcca cgcgagaatc
     3661 cctccgagtg cgccgcccag tgccgcaagc accagagcct cggtgaacac tgaggtcacg
     3721 atcgacccgg gctcgaatcc cagcacgcgc agggtggcaa tttcgaccgc ccgagcgctg
     3781 acggcggcgt acatcgtatt gagagcaccg aacacggcgc cgatggccat gatgctgccc
     3841 accagatagg cgatgaaggt cagcagctgt gcgaaagcgc gggattggtg cagatagtag
     3901 tccgattccc gcatgacctc gatgcccagc gcgggattgc tctccaggga ggcgcggaag
     3961 tcggcgaacg aggcggccga atcgagctgt acggtgaccg actgaaaggt attgcgctgg
     4021 aaggtcgatt gcaggacttc cacgccgctc agcatctccg attcgtgccg atcgcccccg
     4081 ccatcgaacg ctccgactat cgtccattcc gcaccgcgc
//
LOCUS       DASRHN010000271         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1909268, whole genome shotgun sequence.
ACCESSION   DASRHN010000271 DASRHN010000000
VERSION     DASRHN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909268"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFB37_10830"
     CDS             complement(<1..448)
                     /locus_tag="VFB37_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HZO22984.1"
                     /translation="MPRALSRERIGWLALALVVASAAGAEERSRWLQRMNQALTTRNY
                     EGTFSHWHGGRVEMLRIIHRVQDGAVAERLVSLDGSGREFIRKGAELVCYLPDKRTVL
                     VERRPAQESLLSGIPAVNTQTARFYDIQEVAQTRVNRRDTHVITVSP"
     gene            complement(429..1085)
                     /locus_tag="VFB37_10835"
     CDS             complement(429..1085)
                     /locus_tag="VFB37_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HZO22985.1"
                     /translation="MNEELDSQLSAMFDNELPPAECELLARRLSRDEQLKARWGRYAA
                     IGATIRAEGGARLDAEVARRVRMAISTEPELAGTSVRAHTGPLVGRWWRPVAGGAVAA
                     SVAAASILWLRLQTTPEAGSVIAQAAPTRSALTAGGGAAMSAVNVPDSYVVPAKAPSR
                     PSIFPPTQLAKYVVAHSEFSSPVTRRNLLSALMVNESGSASSGPETNEDVEDDAKSPQ
                     "
     gene            complement(1082..1690)
                     /gene="rpoE"
                     /locus_tag="VFB37_10840"
     CDS             complement(1082..1690)
                     /gene="rpoE"
                     /locus_tag="VFB37_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HZO22986.1"
                     /translation="MSADVSDLSLVRRVQRGDKGAFDALVLKYQHKLVKLVMRYVRNP
                     AEAEDIAQEAFIKAYRALPQFRGDSAFYTWLYRIAINTAKNAVVSRDRSPIEYDLDRN
                     SSEESYDMQGRMKDSETPEGLVLTDEIRSTVNAAIEALPEDLRTAIVLRELEGLSYEE
                     IAAAMDCPVGTVRSRIFRAREAIDRRLREVFEGGLGRTEELG"
     gene            complement(1938..2201)
                     /locus_tag="VFB37_10845"
     CDS             complement(1938..2201)
                     /locus_tag="VFB37_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HZO22987.1"
                     /translation="MYTLSQADERRMLWRCRRGMKELDVLLERYVHLHLPGASSKERR
                     AFARLLELPDPLLADYLFGHDLPPEQDLAALAQRISGRPVSAA"
     gene            complement(2203..2991)
                     /locus_tag="VFB37_10850"
     CDS             complement(2203..2991)
                     /locus_tag="VFB37_10850"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZO22988.1"
                     /translation="MADFRLPQNSRLRPGRQHKAPPGVAAHELRTFRIYRFEPDSSEN
                     PRVDTFELDLKGCGPMVLDALIQIKASIDSTLTFRRSCREGICGSCAMNIDGVNRLAC
                     TTSMHDLGARISIFPLPHLPVVKDLVPDLTNFYAQYASVKPWLRSRTPAPPDSERLQS
                     KADQEKIDRPAACILCACCSTGCPSYWWNGDRFLGPAALLAAYRWIIDSRDESAGERL
                     DALEDPFRLYRCHTIMNCTDVCPKDLNPAKAIAEIKKMMLEREG"
     gene            complement(3002..>4482)
                     /gene="sdhA"
                     /locus_tag="VFB37_10855"
     CDS             complement(3002..>4482)
                     /gene="sdhA"
                     /locus_tag="VFB37_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZO22989.1"
                     /translation="EHYGVPFSRTDSGRIYQRPFGGMTTHFGKGIAQRTCAAADRTGH
                     AMLHTLYQQSIRHSATFFIEYFAIDLIMEDGVCRGVVALDLMEGTLHRFRAHMTILAT
                     GGYGRVYFSCTSAHSCTGDGNAMVLRAGLPLQDMEFVQFHPTGIYGAGCLITEGARGE
                     GGYLTNSRGERFMERYAPNAKDLASRDVVSRAMTVEVREGRGVGTERDHIELHLEHLG
                     PEVIHERLPGIAETARIFAAVDVTRQPIPVQPTVHYNMGGIPCNYHGEVVCPKDGNDN
                     RVVPGLMAAGEAACVSVHGANRLGSNSLLDLVVFGREAARRCAEVVRAGAAHKPLARD
                     AGELAVTRLDRLRHAQGSRPTAEIRLEMQKTMQLDAAVFRTAESLNEGMAKLQRTFES
                     FADVRVNDRSLIWNTDLMETLELQNLLLQATATIHSAANRTESRGAQAREDFPSRDDQ
                     NWMKHTLVWVQGPGQTRFDYRPVHLATLTDDVESFPPKARTY"
BASE COUNT          783 a         1485 c         1450 g          764 t
ORIGIN      
        1 tcggtgacac ggtgatcaca tgggtatcgc ggcgattcac ccgggtctgc gccacctcct
       61 ggatgtcata gaagcgtgcc gtctgggtgt tgacggccgg gatgccgctc agcagcgact
      121 cctgcgcagg gcggcgctcc accaggaccg tccgcttgtc gggcaggtag cagaccagct
      181 ccgccccttt tcggatgaac tcgcgccctg agccgtccag ggaaacgagc cgctcggcga
      241 ccgcaccgtc ctgaacgcgg tggatgatgc gcagcatctc cacccgcccg ccatgccagt
      301 gagagaacgt gccctcgtag ttacgagtgg tgagagcctg gttcatccgc tgcagccagc
      361 ggctgcgctc ctcggcaccg gctgcgctcg cgaccacgag ggccagcgca agccatccga
      421 tccgctctct actgagggct cttggcatcg tcctcgacat cctcgttcgt ctcgggcccg
      481 gaagaggcgc ttcctgactc gttgaccatc agggcggaaa gcagattgcg gcgggtgacg
      541 ggcgaggaga actcactgtg cgccacaacg tacttggcga gttgggtcgg ggggaagatg
      601 gagggacgcg agggggcttt ggccggcacg acgtagctgt ctggcacgtt cacggcactc
      661 atcgccgcac cgccgcccgc cgtcagggca gatcgggtgg gagccgcctg ggcgatcacg
      721 gagcctgctt cgggggtcgt ctgcagccgc agccacagga tcgaggcggc cgcgacactc
      781 gcggcaacgg ccccaccggc aactggccgc caccaacggc caaccagggg accggtgtgt
      841 gccctgacgg aggtgcccgc caactccggc tccgtggaga tggccatgcg gacccgccgt
      901 gcgacctcgg cgtccagccg ggccccgccc tcggcgcgga tggtggcgcc gatcgccgca
      961 tagcggcccc atcgggcctt gagctgctca tcccgcgaca ggcgtctggc caacagctcg
     1021 cactcggccg gcggcagctc gttgtcgaac atcgctgaga gctggctatc gagctcttcg
     1081 ttcatcccag ctcctccgta cgacccaggc cgccttcgaa tacttcccgc agacggcgat
     1141 cgatggcctc ccgcgccctg aagatgcgcg agcgcaccgt acccaccgga caatccatgg
     1201 ccgcggcgat ctcctcgtac gacagccctt cgagctcgcg cagcacgatg gccgtgcgca
     1261 ggtcttcggg cagcgcctcg attgcggcgt tcacggtact gcggatttca tccgtcagca
     1321 ccagcccctc cggggtctct gaatctttca ttcggccttg catatcgtag gactcttcgc
     1381 tgctgttgcg gtcgagatcg tattcgatcg ggctgcgatc gcgtgagacc acggcgttct
     1441 tggcggtgtt gatggcgatg cggtaaagcc aggtgtagaa agcgctgtcg ccgcgaaact
     1501 gcggcaacgc gcgataggcc ttgatgaagg cctcctgggc gatgtcttcg gcctccgccg
     1561 gattgcgcac ataccgcatg acgagcttga cgagtttgtg ctggtacttc aggaccagcg
     1621 catcaaaagc tcccttgtcg ccgcgctgga cgcgtcgaac caggctcaga tcactcacat
     1681 cggcgctcat cggcgcctct tactcccgag acaaggccgc cgcgccgcga ccagaataga
     1741 cccggggctg ccgtgaaagt tcaggcggcc ggaggactct cgcgccatcc atcttaagtg
     1801 aaggagatcg tgccgggccg gtggaaaagc aaccgggacc gtgggacctg cgtcacagcg
     1861 cgaccccggc ctcgacctgc ggtcaactgc cgcccgtgac ccctcgagac cgccgcgttg
     1921 gctgcccggg cagggattca cgcggcgctg acgggccggc cagagatgcg ctgtgccaaa
     1981 gccgccaggt cctgctcggg cggaagatcg tgaccgaaca gatagtctgc cagcagcgga
     2041 tcgggcaact cgagcaagcg ggcgaatgcg cgccgctcct tgctgctcgc cccgggtaga
     2101 tgcagatgca cataacgctc gagcagcacg tccagctcct tcatgccacg gcgacagcgc
     2161 cacagcatgc gtctctcatc agcctgcgag agcgtataca tgtcagccct cgcgctcgag
     2221 catcatcttt ttgatctcgg cgatggcctt ggccggattc agatctttgg ggcacacgtc
     2281 cgtgcagttc atgatcgtgt ggcagcggta gagtcggaaa ggatcctcca gcgcatcgag
     2341 ccgctcgccg gcggactcat cccggctgtc aatgatccag cggtaggccg ccaggagcgc
     2401 cgcgggaccc aggaagcgat cgccgttcca ccagtagctc gggcatccgg tcgagcagca
     2461 ggcgcacagg atgcaggcgg ccgggcggtc gatcttctcc tgatccgcct tggactgcag
     2521 tcgctcgctg tcgggaggag ccggggtgcg gctgcgcagc cacggtttga ccgaggcgta
     2581 ctgcgcataa aaattggtca ggtccggaac cagatccttc accaccggca ggtgcggcag
     2641 cggaaagatg ctgatccgcg ctccgaggtc gtgcatcgag gtggtgcagg cgagccggtt
     2701 caccccatcg atgttcatcg cgcaggagcc gcagatcccc tcgcggcagg agcggcggaa
     2761 ggtgagagtg gaatcgatgc tcgccttgat ctggatcagc gcatccagaa ccatcggtcc
     2821 gcaacccttg agatcgagct cgaaggtatc cacgcgcggg ttctcgctgg agtccggctc
     2881 gaagcgatag atgcggaagg tgcgcagctc gtgcgccgcc acacccggcg gagccttgtg
     2941 ctgccgcccc gggcgtaagc gagagttctg cggaagtcgg aaatcggcca tgcctctttg
     3001 ctcagtaggt gcgcgccttg ggagggaagg attccacgtc atcggtcaga gtcgcgagat
     3061 gcacgggccg gtaatcgaag cgcgtctgac ccggaccctg cacccacacg agggtgtgct
     3121 tcatccagtt ctgatcgtcg cggctcggga agtcctcgcg cgcctgcgcg ccgcggctct
     3181 cggtgcgatt ggccgcggag tgaatggtcg ccgtcgcctg caggagcagg ttctgcagct
     3241 cgagcgtttc catcagatcg gtgttccaga tcagcgagcg gtcattgacg cgcacgtccg
     3301 cgaagctctc gaacgtgcgc tgcagcttcg ccatgccctc attcagcgac tccgcggtcc
     3361 ggaagacggc cgcatcgagc tgcatggtct tctgcatctc gagccggatt tccgccgtag
     3421 gtcgtgagcc ctgcgcatga cgcaggcgat ccaggcgtgt cacggcaagc tcaccggcat
     3481 cgcgcgccag cggcttgtgg gcggctcccg cgcggacgac ttctgcgcag cggcgggccg
     3541 cctcgcgacc gaacacaacc agatccagca gcgagttgga gcccagccgg ttggcgccat
     3601 gcaccgagac acaggccgcc tcgcccgcgg ccatgagtcc aggcaccaca cgattgtcgt
     3661 tgccgtcctt gggacaaacg acctcgccat gatagttgca gggtatgccg cccatgttgt
     3721 agtggacggt cggctgcacg ggaatgggct ggcgagtcac atccacggcc gcgaagatcc
     3781 gtgccgtctc ggcaatgccc ggcaggcgct cgtgaatcac ttcggggccc agatgctcca
     3841 gatgcagctc gatgtgatcg cgctcggtgc ccacaccgcg cccttcccgg acctcgacgg
     3901 tcatcgcacg gctcacgaca tcccgggaag cgaggtcctt cgcattgggc gcatagcgct
     3961 ccatgaaacg ctcgccgcgt gagttggtga gatagccgcc ctcgccgcgc gcgccctccg
     4021 tgatcagaca tcctgcgccg tagattcccg tcggatggaa ctggacgaac tccatgtcct
     4081 gcagcggcaa gcccgcgcgc agcaccatgg cattgccgtc gcccgtgcac gagtgcgccg
     4141 aggtacagga gaaatacacg cgaccgtagc cgccggtcgc gagaatggtc atgtgggcac
     4201 ggaaacggtg cagcgtgccc tccatgagat ccagagcgac cactccacgg cacacgccat
     4261 cctccatgat gagatcgatc gcaaagtact cgatgaagaa ggtcgccgag tgccggatcg
     4321 actgctgata caacgtgtgg agcatcgcat gcccggtacg atcggccgct gcacaagtgc
     4381 gctgcgcaat ccccttaccg aaatgcgtcg tcatcccgcc aaacggccgc tgatagatac
     4441 gcccgctatc ggtgcgcgag aagggcacac cgtagtgctc ca
//
LOCUS       DASRHN010000272        10929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2235047, whole genome shotgun sequence.
ACCESSION   DASRHN010000272 DASRHN010000000
VERSION     DASRHN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235047"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFB37_10860"
     CDS             complement(<1..194)
                     /locus_tag="VFB37_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO22990.1"
                     /translation="MALKIMILGANGFIGSALTNAILRTRPWEVYGIDLADNKLKDLP
                     PTDRFHFVEGDITINKEWVE"
     gene            complement(368..1252)
                     /locus_tag="VFB37_10865"
     CDS             complement(368..1252)
                     /locus_tag="VFB37_10865"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZO22991.1"
                     /translation="MPTLADKVAAREFVVTTELVPPKGTDLSEVFARAQLLRAHVDAF
                     NLTESPRARMAIAPFAVARLLLERDVEPIVQVTARDRNRIAIQSDVLGAAALGVRNFV
                     FMAGDPPTTGDHPDAKPVFDLNSNQMLRAAHALAQGRDMAGNPLRGAPNLFLGATANP
                     GAADLEGEVTNTRRKIDAGAQFLQTQAIYERDTLERFLERVKPDGVAILAGIIPLKSA
                     RMAAWLDANLPGVRVPERLQQEMLSVSGTNAEVGRGIEIAARTISEVQALCAGVHLMT
                     LGWEEHIPAILAASSIRS"
     gene            1346..2170
                     /locus_tag="VFB37_10870"
     CDS             1346..2170
                     /locus_tag="VFB37_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZO22992.1"
                     /translation="MRSVFGREALLSVGALLISAIVVQSLYATIIRPRAEAILALPAV
                     PEAAEPGTPAGAQRPPTRNLRSVFVILKDYEQETALILACWALALIGYQALAVTRNRR
                     LLAKQYVAIGEGHVVLPDDARAYARPLESLPREEQDLLLPRALMVALNRFGATRSVQD
                     AAEAVRAECENELDRLDAQLSMVRFTAWAIPAVGFVGTVRGIGRALQEAQGALHGDIS
                     GVTLGLGVTFNATLTALVACILVMFFLHQLQQAQDRLVLDARMYIDRRLIRHMRVH"
     gene            complement(2190..3545)
                     /locus_tag="VFB37_10875"
     CDS             complement(2190..3545)
                     /locus_tag="VFB37_10875"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006683.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HZO22993.1"
                     /translation="MTSLWFESALLPQGWASRVRISSAGGRIEQIAVGSDPADGDERH
                     AVGLPGLPNLHSHAFQRALAGLTERAGPTGDSFWTWRELMYQFVDRLDPDELEAIASL
                     AYAEMLEGGLTHVGEFHYLHHDRSGVPFADPGELAGRLVAAAKLTGIGLTLLPVLYAH
                     GGFGGAAPSARQQRFITDPSRFEAILESCRRAVKALSGGVAGIAAHSLRAVTPEELTW
                     LVQAAGDNLVHIHIAEQIKEVEDCKAWSGQRPVEWLLEHSPVGERWCLVHATHVTQTE
                     LEGIAATGAVIGLCPITEANLGDGIFPARAFLERAGRIGIGSDSNVLLDGAEELRTLE
                     YSQRLAHRARNVLAPKPGSSTGRSLFDAVLAGGAQALGCASGFAPGACLDAISLDRHH
                     PSLAGRREDELLDSWIFAGGRGLIDCVWRAGEKVVAGGCHRRREPIVQRYRQVLKRVL
                     G"
     gene            3587..4420
                     /gene="hutG"
                     /locus_tag="VFB37_10880"
     CDS             3587..4420
                     /gene="hutG"
                     /locus_tag="VFB37_10880"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR02017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HZO22994.1"
                     /translation="MAVETLPPWLTVLRGEAPLITSMPHTGVDIPAPIESQLVSPWLA
                     RKDADWWIDRLYDFAHALGATVIRTGFSRTVIDVNRDPSGASLYPGQATTELCPTTTF
                     DGEPLYRPGQGPQSEEIASRRATWFDPYHAAIRAEIQRLRGRYPRVVLYDCHSIRSRI
                     PRLFEGTLPNFNIGTNQGSTCAPTLTTAVEQVCATTAFSRVTNGRFRGGYTTRHYGRP
                     GEGVHALQMELACRGYMREPMQQVSASEWPTPYDEALARPMRAALTDILKICIDFGRA
                     R"
     gene            complement(4436..6073)
                     /gene="murJ"
                     /locus_tag="VFB37_10885"
     CDS             complement(4436..6073)
                     /gene="murJ"
                     /locus_tag="VFB37_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZO22995.1"
                     /translation="MSPEASSSAEPAAQAIRAEKLDTRAAGVVGLAVLCSRILGLARE
                     QIFAALFGGGRLMDAFTIAFRIPNLLRDLFAEGALSTSFVTTFSKTIATDGDAAAWRL
                     ANKVATLTAIFLSAITIIGIASAPWLVSVLAPGFEGEKAALTVTLTRIMYPFILLVSL
                     AALVMGILNSKRIFGIPAMASSFFNLGSIIAGVSLGWWLDPQFGARALLGAALGTLIG
                     GALQLGVQIPAVRRLGYVFHPDFLWRDSGVRAILRLMGPSVVAASTTQVNVLVNSIFA
                     SRLGDGPTFWLTIAFRLMQLPLGIFGVALGTVALPLLARMAAAGNTTAFRSELARGMR
                     LAFLMTIPASVGLIVLAEPIISVLYQHGRFGAHETAEAAGALRFYALGLCGYAALKVL
                     VNAFYALDHRKTPMVVSFLAVGLNLLLNWIFTLHLGWGHRGLAFSTACVATTNFLILY
                     VLMRRRLRFLESSVMLLLLLKVAAASLVLAGICWAGAHYLLADWATQRFGPKLLELLG
                     VIVTAGAAFFLCAATMGIPELRDIGAALQRRLRRRRG"
     gene            6189..7088
                     /locus_tag="VFB37_10890"
     CDS             6189..7088
                     /locus_tag="VFB37_10890"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZO22996.1"
                     /translation="MNSDVIITCAVTGDDTKVTKSPHCAITPAQIAATAVEAAQAGAA
                     IVHIHVRDPETGAFSMETKYYREVIKRIRESKVDVIVNLTCGMGGYICVGDNGLSDTP
                     APGTDFVSQEARMRHIIELCEEGQYRPEIATLDCGSLNFGDGNRAYISTPNYLRKGAS
                     ILRDLKVKAELEVFDTGNLWFVKQLIKEDLIPSPALIQLCTGIPYGVPAEVGHLLAMV
                     NSLPQDAIWTSFAISRMQMPWVAQSVLLGGNVRVGLEDNLYLSRGVYASNAQLVQKAR
                     TIIEAMGARILNPSEARERMKLR"
     gene            7085..7564
                     /locus_tag="VFB37_10895"
     CDS             7085..7564
                     /locus_tag="VFB37_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZO22997.1"
                     /translation="MSGAALYRTPIHGDWIDYNGHLRDAYYGLIVSLATDELMDRLGI
                     DASYRQSSGNTLYTLELHMHFLREVKQSDIVQVTVRILGSDTKRIHAALDLNCEGRSE
                     PAATAEIMLLHVHQGDAPAAQPFPAPIAAALAKFALTHGALAPGIPGSRRMELRSRS"
     gene            7561..9627
                     /locus_tag="VFB37_10900"
     CDS             7561..9627
                     /locus_tag="VFB37_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZO22998.1"
                     /translation="MTPSALSRLLAPRTLALFGGDWADAVEAAGRAVGYRGEVWRVHP
                     SRSSTGTARYFRSVEELPGAPDASFVAAPAHEVAAIAAALARRNAGGFVCFAAGFSET
                     GTEPGQQLTTDLSRSAGNLPFFGPNCYGFLNFFDRVALWPDQVVGSPLERGVALICQS
                     GTLALNLLFSDRSLPIGYLLTVGNQTCLAVEDLIEALCEDERVSAFGLYVEGVKDGVR
                     FAKAVERAHQAGKPIALVKAGRTQSAARTVHTHTGSLAGADSVFDSFCRQAGIARCES
                     LATLCETLKILHTGGPLPGRRLLVMGASGGDMAMAADLARHLNLSFPPFEPASLTRLR
                     EILTDRVTLSNPFDFHTHVWFDRPALRALFEVAHQSGFDATGLVIDCPPAQHADPAAY
                     LAVIEEFISAYPGAPARAAVISSLPESMAPATRQRCLQAGIIPLQGQREALEALDLAA
                     QVGEGWPSTARVSLHRRALRGACGSARTLTEIESKAALSLHGVRIPRSRVVAPEDAGG
                     AAESIGFPVALKAIGAHLEHKSELGAVILNLRSRKEVQAAAQRLARLSDAILVEEMIV
                     DAVAEILVGVTHDPQFGQALVLGAGGVLTELLRDSVTLLPPFNAVGIEAGLRQLKAWK
                     LLEGYRGKPPGDIGGLVAIGLGCANYAAAHIERLVELDVNPVIVRPVGLGAVAVDALI
                     RLAEET"
     gene            9629..10423
                     /locus_tag="VFB37_10905"
     CDS             9629..10423
                     /locus_tag="VFB37_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO22999.1"
                     /translation="MSELSTYRDGIRTTQAEAVLEITLDRPKANAIDLAASRRLNAIF
                     SQFRDDPALRIAIITGAGERFFSPGWDLKAAAAGEESDSDWGVGGFGGLNYPRNLDKP
                     IIAAVNGIACGGGFEIVLGTDIIVMEEHARFALPEINVAVLADAATIKLRRRIPYHVA
                     VEFLMTGRWMEAAEAKHWGLANHIVPTGQGLAKACEIARQLAAGPPLLYPAIKQLLRH
                     TEMVPENLAFEVHDALHAVQKVIRSEDLKEGARAFSEKRPPVWKGR"
     gene            complement(10461..>10929)
                     /locus_tag="VFB37_10910"
     CDS             complement(10461..>10929)
                     /locus_tag="VFB37_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZO23000.1"
                     /translation="HKGFEVANTWLGATRLQVAANCLGRAERALELATQWAADRVQFG
                     QQIGKFQGVAFKLADMAVELRAAELLTLEAAWKLDRGCSSEMDMAIAKLKATEMLAMV
                     ADEALQIHGGMGLMSDLPLERIWRDARIERIWDGTSEVQRHIISRALLRPLGG"
BASE COUNT         1908 a         3669 c         3550 g         1802 t
ORIGIN      
        1 tactccaccc actccttgtt gatggtgatg tcgccttcga cgaagtggaa tcggtcggtg
       61 ggcggcagat ccttgagctt gttgtcggcg aggtcgatgc catagacctc ccagggccgc
      121 gtgcgcagga tagcgttggt gagcgcgctg ccgatgaagc cgttcgcgcc gagaatcatg
      181 attttcagtg ccatgcgtcc ttacccctgt cgttcggggg ggcgcagctt accatgggcc
      241 accgcagggg ccgggccgcg ctaaagcttt cgcaggcgaa aggtggaaag tgcggcacgg
      301 gtagctcgca gccggggtgc gggcatgctg ttggcaacag cgcgaccgca gcttctccga
      361 ggcgatgtca gctccggatc gagctggccg caaggatcgc cgggatgtgc tcttcccaac
      421 cgagcgtcat cagatgcacg ccggcacata gggcctgcac ttcgctgatc gtccgggcgg
      481 cgatctcgat gccccggccc acttcggcgt tcgtgcccga gacgctcagc atctcctgct
      541 gcaagcgttc cggcacgcgc acgccgggaa gattcgcatc gagccaggca gccatgcgtg
      601 cggatttgag tggaatgatg ccggcgagga tcgcgacccc gtcaggtttg acccgctcca
      661 gaaatctctc cagcgtgtcc cgctcgtaga tggcctgggt ctgcaggaac tgcgcgccgg
      721 catcgatttt gcgccgggtg ttggtgacct cgccttcgag gtcggctgca cccgggttgg
      781 cggtggcacc gaggaacaga ttcggcgcac cgcgcaaggg gttgcccgcc atgtcgcggc
      841 cctgggccag ggcatgggcg gcgcgcagca tctgattgga gttcaggtcg aagaccggct
      901 tcgcatccgg gtgatcgccg gtggtgggcg gatcgcccgc catgaacacg aagttgcgaa
      961 cgccgagggc ggccgccccc agcacgtcgg attgaatggc gatccggtta cgatcgcgcg
     1021 cggtgacctg cacgatgggc tcgacgtcgc gctccaggag caggcgggcc acggcaaacg
     1081 gggcaatcgc catccgggcg cggggcgact cggtgaggtt gaaggcatcc acgtgggcac
     1141 gcaggagctg cgcgcgggca aacacttcgc tgagatcggt tcccttgggc ggtacgagct
     1201 cggtcgtgac gacgaactcg cgggcggcga ccttgtctgc aagcgtgggc acgggaggct
     1261 ttcgatgaat cggagcggcc ggtagcctac tctattggaa aagccgccct gcggccgcaa
     1321 aagcctgcac acggagtcgg gattgatgcg ttctgtattc ggacgtgagg cgctcctgag
     1381 cgtgggcgca ctgctcatca gcgccatcgt ggtgcagagc ctgtacgcca ccatcatccg
     1441 gccccgcgcg gaagccatcc tggcgctgcc cgctgtcccg gaggcggccg agccagggac
     1501 acccgccggc gcgcagcgtc cacccacgcg caatctgcgt tcggtgttcg tcatactaaa
     1561 ggattacgag caggagactg cgctgatcct ggcgtgctgg gccctggctc tgatcggcta
     1621 tcaagcgctc gccgtcacgc gcaaccgccg gctgctcgcg aagcagtatg tcgcgatcgg
     1681 agaaggccat gtcgtgctgc cggacgatgc gcgtgcctac gcgcgcccgc tggagagcct
     1741 gccgcgcgag gaacaggatc tgctcctgcc ccgcgccctg atggttgcgc tgaatcgatt
     1801 cggcgccact cgcagcgtgc aggacgccgc ggaggccgtg cgagcggagt gcgagaacga
     1861 gctcgatagg ctcgatgccc agctttctat ggttcgcttc acggcatggg ccattccggc
     1921 cgtcggtttc gtgggcacgg tgcgcggcat cggtcgcgcg ctacaggagg cgcagggcgc
     1981 gctgcacggg gacatctccg gtgtgacgct cggactcggc gtcacattca acgccacgct
     2041 gaccgcgctg gtggcctgca tcctcgtgat gttcttcctg catcagctcc agcaggctca
     2101 ggaccggctc gtgctcgatg cccggatgta catcgatcgg cggctcatcc ggcacatgcg
     2161 ggtgcattga tgcggcggct ccgccgcgct cagccgagta ctcgcttcag cacctgacga
     2221 tagcgctgca caatcggctc gcgccggcga tggcatccgc cggccacgac tttctcgcca
     2281 gctcgccaga cacagtcgat cagcccccgg ccgccggcaa atatccagct gtcgagcagc
     2341 tcgtcctcgc gccgccccgc cagcgaggga tggtggcgat cgagactgat ggcatcgagg
     2401 caggcgcctg gcgcaaagcc actggcacaa cccaatgcct gggcgccgcc ggcgagcacc
     2461 gcatcgaaaa gcgagcgccc ggtcgagctg ccgggtttcg gagccaggac gttccgcgcc
     2521 cgatgtgcga gccgctggga atattcgagc gtccgcagct cctcggcccc atccagcagc
     2581 acgttggaat ccgatccgat gccgatgcgg ccagcgcgct cgaggaacgc ccgcgccggg
     2641 aagattccat ctcccaggtt ggcttccgtg atggggcaca agccgatcac cgcgcccgtt
     2701 gccgcaatgc cctcgagctc tgtctgggtg acgtgcgtgg cgtgcacgag gcaccatcgc
     2761 tcaccgaccg gcgaatgctc gagcagccac tccacagggc gctgtcccga ccacgccttg
     2821 cagtcctcca cctccttgat ctgctccgcg atgtgaatgt gcacgaggtt gtcgccagcg
     2881 gcctgcacca gccatgtcag ctcttcaggg gtcacggcac gcaggctgtg cgcggcaatc
     2941 cccgcaacgc cacccgatag cgctttcacg gcgcggcggc acgactccag gatggcctcg
     3001 aatcggctcg gatcggtgat gaagcgctgc tggcgcgcgg aaggcgccgc ccctccgaaa
     3061 ccaccgtgcg catacagcac cggcaggagc gtaagaccga tgccagtgag cttggcggcg
     3121 gcgacgagcc ggcctgcaag ctcgccggga tccgcaaagg gcacgccgct gcgatcgtgg
     3181 tgcagataat gaaactcgcc cacgtgcgtc aagccgcctt cgagcatctc ggcataagcg
     3241 agcgaggcga tcgcctccag ctcatccggg tcgagccgat cgacgaattg atacatcagc
     3301 tcccgccagg tccagaagct gtcgcccgtc ggtccggcgc gctcggtgag gcccgccagg
     3361 gcgcgctgaa aggcgtgact gtgcaggttg ggaagacccg gtagacccac cgcatgccgc
     3421 tcgtcgccat ccgccggatc gctccccacg gcaatctgct cgatgcggcc gcccgcggat
     3481 gaaatgcgca cgcggctcgc ccaaccttgc ggcagcagag ccgattcaaa ccacagactc
     3541 gtcatgccgc tcgtcactcc aggtccgcct tcgctattct atccccgtgg ctgtcgaaac
     3601 cctccctccc tggctgaccg tgcttcgtgg cgaggcgcct ctcatcacga gcatgccgca
     3661 taccggcgtc gatatcccgg cgcccatcga atcgcagctc gtctccccct ggttggcgcg
     3721 caaggatgcc gactggtgga tcgaccggct ctatgacttc gcccacgctc ttggagccac
     3781 cgtgatccgc accggctttt cccgaacggt catcgatgtg aatcgcgacc cttcgggggc
     3841 ctcgctctac ccgggacagg ccaccactga gctgtgcccc acgacgacct tcgatggcga
     3901 gccgctgtac cgcccgggcc aggggcctca atcggaagag atcgcctcgc gacgcgccac
     3961 ctggttcgat ccgtaccacg cggcgatccg ggccgagatc cagcggctgc gcggccgcta
     4021 cccgcgtgtg gtgctgtacg actgccactc gatccgctca cgcattcccc ggctgttcga
     4081 gggtacgctg ccgaatttca acatcggcac gaatcagggc agcacgtgcg cgccgactct
     4141 gaccacagcg gtcgagcagg tctgtgccac gaccgccttc tcacgggtga ccaatgggcg
     4201 cttccgggga ggctacacga cgcgacacta cggcaggcct ggcgaaggcg tgcatgcgct
     4261 gcagatggag cttgcctgtc gcggctatat gcgcgagccg atgcagcagg tgtcagcctc
     4321 cgaatggccc acgccttacg atgaagcgct cgcccgcccg atgcgcgcag cgctcaccga
     4381 cattctgaag atctgcatcg acttcggtcg cgcccgctga gcgctcttgc tgcgctcatc
     4441 cgcgccggcg acgcaatctg cgctgcaaag cggcgccgat atcacgcagc tcggggatcc
     4501 ccatcgtagc cgcgcagagg aagaaggcgg cgcctgccgt gacgatcacc ccgagcagct
     4561 ccagcaactt gggcccgaat ctttgcgtgg cccaatccgc cagcaggtag tgcgcacccg
     4621 cccagcagat tcccgccagg acgagactcg ctgcagccac cttcagcagc agaaggagca
     4681 tcacgctcga ctccaggaac cgcaggcgcc ggcgcatgag cacatagagg atcaggaagt
     4741 ttgtggtcgc cacacaggcg gtcgaaaagg ccagtccgcg atgaccccag cccaggtgca
     4801 gcgtgaagat ccagttcagg agcagattga gccccacggc caggaagctc acgaccatcg
     4861 gggtcttgcg gtgatcgagt gcatagaagg cattcaccag caccttcagg gccgcgtaac
     4921 cgcacaggcc gagagcgtag aaacgcagag cgccggcggc ctcggccgtc tcgtgtgcgc
     4981 cgaacctgcc gtgctgatag aggaccgaga tgatcggctc ggccagcacg atcaatccaa
     5041 cactggccgg gatggtcatc agaaaggcga gccgcatgcc gcgcgcgagc tcactgcgaa
     5101 atgcagtcgt attcccggct gccgccatac gggcgagcag aggcaacgcc accgtgccga
     5161 gcgcgacccc gaagatcccc agcggcagtt gcatgagacg aaacgcaatg gtcagccaaa
     5221 aagtggggcc atcgcccagg cgcgaggcga agatcgagtt cacgagcaca ttcacttggg
     5281 tcgtgctggc tgcaacgacc gaggggccca tgagacgcag gatggcccgt accccggaat
     5341 cccgccacag aaagtccgga tgaaacacgt agcccaaccg cctcaccgcc gggatctgca
     5401 cacccagctg cagcgcgccg ccgatcagcg tgcccagggc tgcgccgagg agtgcccgcg
     5461 cgccaaactg cggatcgagc caccagccga ggctgactcc ggcgatgatc gagccgagat
     5521 tgaagaagct cgaggccatg gccggaatcc cgaagatccg cttcgagttc aggatgccca
     5581 tcaccagggc cgcgagggac acgagcagaa tgaacggata catgatgcgc gtcagggtga
     5641 cggtgagcgc agccttctcg ccctcgaatc cgggtgcgag cacgctcacc agccaggggg
     5701 cggaggcgat gccgatgatc gtgatcgcgc tgagaaagat cgccgtgagc gtggccacct
     5761 tgttcgcaag ccgccaggcc gccgcatccc cgtcggtcgc aatcgtcttg ctgaaggtcg
     5821 tgacgaacga agtcgagagc gccccctcgg cgaacagatc gcgcagcaga ttggggatgc
     5881 gaaaggcgat ggtgaaggca tccatcagcc ggccgccgcc gaagagcgcc gcgaaaatct
     5941 gctcgcgcgc cagcccgagg atgcggctgc acagcaccgc cagacccacc acccccgcgg
     6001 cgcgcgtatc gagcttctcg gcgcggatgg cctgcgcagc gggctctgcg ctgctggatg
     6061 cttcgggtga catgcggagg cgacggtagc acacgtcgcc gggggctatg cctgggcggg
     6121 tccgattgct atattattgc aacgataata ttacccggcg gaccccttca accccacagg
     6181 cgacccacat gaattccgac gtcatcatca cgtgcgccgt gaccggcgac gacaccaagg
     6241 tcaccaagag cccgcactgc gccatcacgc cggcgcagat cgccgcaacc gcggtcgagg
     6301 cagcccaggc cggcgctgcc atcgtgcaca ttcacgtgcg cgatcccgaa accggcgcct
     6361 tcagcatgga gacgaagtac taccgtgagg tcatcaagcg cattcgcgag agtaaggtgg
     6421 acgtcatcgt caacctcacg tgcggcatgg gtggctacat ctgcgtcggt gacaacggtc
     6481 tgtcggatac tcccgccccc ggcacggact tcgtgagcca ggaggcgcgc atgcgccaca
     6541 tcatcgagct gtgcgaggaa ggccagtatc ggccggagat cgccacgctc gattgtggct
     6601 cgctcaattt cggcgatggc aaccgcgcct acatctcgac gcccaattac ctgcgcaagg
     6661 gcgcatccat cctgcgcgat ctgaaggtga aggcggagct ggaggtcttt gacaccggta
     6721 acctgtggtt cgtcaagcag ctcatcaagg aagatctgat cccctccccc gccctcatcc
     6781 agctctgcac cggcattcca tacggcgtgc ccgccgaggt gggtcatctg ctcgcgatgg
     6841 tcaactctct gcctcaggat gcgatctgga cctccttcgc gatcagccgg atgcagatgc
     6901 catgggtggc gcagtcggtg ctgctcggcg gcaacgttcg cgtcgggctg gaagataatc
     6961 tctacttgag ccgcggggtc tacgcgagca acgcgcagct ggtgcagaaa gcccgcacca
     7021 tcatcgaggc catgggggcg cgcattctca acccctccga ggcccgtgag cgaatgaagc
     7081 tcagatgagc ggtgcagccc tgtatcgcac cccgatacac ggggactgga tcgattacaa
     7141 cggccacctg cgtgatgcct actacggcct gattgtcagc cttgcgaccg acgagctgat
     7201 ggatcggctc ggcatcgacg cgagctatcg gcagtccagc ggcaacacgc tgtacacact
     7261 ggagctgcac atgcattttc tccgggaggt caagcaatcg gacatcgtac aggtcacggt
     7321 gcgaatcctg ggaagcgaca ccaagcgcat tcacgcagcg ctcgatctga actgcgaggg
     7381 gcggagcgag cctgccgcca cggcggaaat catgttgctg catgtccatc agggcgatgc
     7441 gcccgccgca cagcctttcc cagcgccgat cgccgcggcg ctcgcgaagt ttgccctgac
     7501 ccatggagcc ctcgcgcccg gcatcccggg atcgcggcgc atggaactgc gcagccgctc
     7561 gtgaccccca gcgcccttag ccgtctgctc gcgcctcgca cgctcgccct gttcggcggc
     7621 gactgggcag atgccgtcga ggctgccggg cgcgccgtgg gctatcgcgg tgaggtctgg
     7681 cgagtgcacc cgagtcgatc ctccactggc actgctcgct acttccgctc ggtagaggag
     7741 cttccgggag cgccggatgc atcgttcgtg gcggcccctg cgcatgaggt cgccgcgatt
     7801 gccgccgcgc tggcgcgccg caatgcgggc ggcttcgtct gcttcgcggc agggttttcc
     7861 gagaccggca cggagccggg acagcagctg acaacggacc tcagccgcag cgccgggaac
     7921 ctgcctttct tcggaccgaa ctgttatgga tttctgaact tcttcgatcg cgtggcgctg
     7981 tggccggacc aggtggtggg cagcccgctc gagcgaggcg tcgcgctcat ctgccaaagt
     8041 ggcacgctcg cattgaatct gctcttcagt gatcgatcgc tccccatcgg ctacctgctc
     8101 accgtgggca accagacctg tctggcggtc gaagatctga tcgaagcgct gtgcgaggac
     8161 gagcgcgtca gcgcattcgg actttatgtc gagggtgtga aggatggcgt gcgctttgcc
     8221 aaggcggtgg aacgagctca tcaggcaggc aaaccgattg ccctcgtcaa ggcggggcgc
     8281 acgcagtctg ccgcccgcac ggtgcacacc cacaccggct ctctggcggg ggccgattcg
     8341 gtgtttgatt cgttctgccg ccaggcgggg atcgcccgct gcgagtcgct cgccaccttg
     8401 tgcgagactc tcaagatcct gcacaccggg ggcccgctgc cagggcggcg gctgctcgtg
     8461 atgggcgcct ccggcggtga tatggccatg gcagcggatc tggccaggca cctgaatctc
     8521 agcttcccgc cgttcgagcc cgcttcgctc acccgactgc gcgagatcct gaccgatcgg
     8581 gtcacgctgt cgaatccctt cgactttcac acgcacgtct ggttcgaccg cccagcgctg
     8641 cgggctctgt tcgaggtcgc gcaccagtcg ggcttcgatg ccacgggact cgtcatcgac
     8701 tgcccgcccg cgcaacacgc ggaccccgct gcctacctcg cggtgatcga ggagttcatc
     8761 agcgcctatc cgggcgcacc ggcgcgcgcc gccgtgatct cttccctgcc cgagtcgatg
     8821 gctcccgcca ctcggcagcg ctgcctgcag gccggaatca tcccgctgca gggccagcgc
     8881 gaggcgctcg aggctctcga cctcgctgcg caggtgggcg agggctggcc gagcaccgcc
     8941 cgcgtgagct tgcatcgacg cgcgctccgc ggcgcctgcg gatcggcgcg cacgttgacc
     9001 gagatcgaga gcaaagctgc gttgtctttg cacggcgtac gcatcccgcg ctcgcgcgtg
     9061 gtcgctccgg aggatgcggg aggggcggcc gagagcatcg gctttccggt ggccctcaag
     9121 gcaatcggtg cgcacctgga gcacaaatcc gagctcggcg ccgtgatcct caacctgcgc
     9181 agccgcaaag aggtgcaggc cgcagcgcag cgactggcaa gactctcgga tgcgattctc
     9241 gtggaagaga tgatcgtgga cgcagtggca gagatcctgg tcggcgtgac gcacgatccc
     9301 cagttcgggc aagctctcgt gctcggcgcc ggcggggtac tcacggagct gctgcgcgac
     9361 agcgtgacac tgttgccccc tttcaatgcc gtgggcatcg aagccggact gcgccagctc
     9421 aaagcctgga agctgctgga gggttatcgt ggcaagcccc ccggggatat cggggggctc
     9481 gtagcaattg gactgggctg cgcgaactac gccgcagccc atatcgagcg gctcgtggag
     9541 ctcgacgtca atccggtgat cgttcggccc gtcgggttgg gcgccgtcgc cgtggacgcc
     9601 ctgatccggc tcgccgagga gacatgacat gtcggagctg agcacgtatc gcgatggtat
     9661 tcgtaccacg caggcggagg cggtactgga gatcacgctc gatcgtccca aggccaacgc
     9721 gatcgatctg gccgcaagcc ggcggctgaa cgccatcttc agccagttcc gcgatgaccc
     9781 ggcgctgcgc atcgcaatca ttaccggggc gggtgagcgc ttcttcagcc cgggctggga
     9841 tctgaaggcc gccgctgcgg gtgaggaatc cgactcggac tggggcgtag gcggattcgg
     9901 tgggctgaat tatccgcgca atctcgacaa gccgatcatc gcggccgtga acggcatcgc
     9961 ctgcggcggc ggctttgaga tcgtgctcgg caccgacatc atcgtcatgg aggagcacgc
    10021 aagattcgcg ctgcccgaga tcaacgtcgc agtgctcgcc gatgcggcga ccatcaagct
    10081 gcgacggcgt atcccctatc acgtcgcggt ggagttcctg atgaccgggc gctggatgga
    10141 agccgccgaa gccaagcatt gggggctggc caatcacatc gttcccacgg ggcagggcct
    10201 ggccaaggcg tgcgagatcg cccgacagct cgccgcgggc cctcccttgc tgtatccggc
    10261 aatcaagcag ctcctgcgcc ataccgagat ggtccccgag aatctcgcct tcgaggttca
    10321 cgacgcgctg catgcggtgc agaaagtcat tcgctccgag gacctgaagg aaggcgcgcg
    10381 cgccttcagc gagaagcgcc ccccggtctg gaaaggtcgc tagcgccggc atcgccggcc
    10441 caaaagccgc cggcgcaagt tcaaccgccg agaggccgca gcaacgcccg cgagatgatg
    10501 tgccgttgca cctcgctcgt cccatcccag atgcgctcga tgcgagcatc gcgccagatc
    10561 cgctcgagcg gcagatccga catcagcccc atcccgccat ggatctgcag ggcctcatcg
    10621 gcgaccatgg cgagcatctc ggtcgccttc agcttcgcga tcgccatatc catctcgctc
    10681 gagcagccgc gatcgagctt ccaggccgcc tccagtgtga gcagctccgc ggcgcgcagc
    10741 tccacggcca tgtctgccag cttgaacgcg acgccctgaa acttgccgat ctgctgcccg
    10801 aattgcaccc ggtccgcggc ccactgtgtg gcgagctcga gcgcgcgctc cgccctgccc
    10861 aggcagttgg ctgcgacctg caggcgcgtg gcgccgagcc aggtattggc gacctcgaag
    10921 cccttatgg
//
LOCUS       DASRHN010000273        25830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2235155, whole genome shotgun sequence.
ACCESSION   DASRHN010000273 DASRHN010000000
VERSION     DASRHN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 25830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25830
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235155"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VFB37_10915"
     CDS             complement(<1..819)
                     /locus_tag="VFB37_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZO23001.1"
                     /translation="MQQQSGHAPLRVPARLGDYSVNPRIVIISALALAVGAVAALVAD
                     GLLKLIGLITNLVFYQRVATGLVAPGSGVHPWWLIIGAPVVGGLIVGLMARYGSEAIR
                     GHGMPEAIEAIMRGGSKIRPRVALLKPVSAAISIGTGGPFGAEGPIIMTGGAFGSLMA
                     QTLSLTADERKTLLVAGAAAGMAATFNAPLASILLAVELLLFEFRPRSFVPMSIAVVV
                     ATALRWRLLGVGALFTTQHALPVLTSTDLLLCAAAGVVGGFVAWFATLLVYLSEA"
     gene            complement(1071..1379)
                     /locus_tag="VFB37_10920"
     CDS             complement(1071..1379)
                     /locus_tag="VFB37_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZO23002.1"
                     /translation="MSKSCSHLKSIVVVKPSAKGCEECLKMGTRWVHLRICRKCGHVG
                     CCDDSPERHATKHFHATRHPIIEGYDPPEGWGWCYIDEVFIDLGENTTPQVGPIPRYV
                     "
     gene            1607..2653
                     /locus_tag="VFB37_10925"
     CDS             1607..2653
                     /locus_tag="VFB37_10925"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZO23003.1"
                     /translation="MFSVTALLAGAWGLAFVFLNVLLEQAGLPIPAVPTLVLAGALAA
                     KRPEWGLAAFALALAACVITDVAWYGAGRAYGGRIVRLLCRISLSPDSCVSETQLRFE
                     RWGARALLLSKFVPGLATLAPSLSGALRMQLSLFVGMSALGSALWVIAFMVLGTLAAP
                     QITALLPQVAALGGRAAILIALLLVLYILFKWVQRQRVYAELRMARVEVGELYLMLQR
                     VPAPTILDVRSMSAHALDPRAIPGAVHVPPEDIAARIAAVEANAEVVVYCNCPNEASA
                     ARVAQLLRRHGVVRVRPLLGGLDAWVAAGYLVTDTRSAPAAPAPSLASSRPAAATDLP
                     KEPEAPSPRQRSSS"
     gene            complement(3070..5523)
                     /locus_tag="VFB37_10930"
     CDS             complement(3070..5523)
                     /locus_tag="VFB37_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO23004.1"
                     /translation="MSEPASSDATSLPSHARVVVIGGGVVGCSILFHLAKHGWRDCIL
                     LERQELTSGSSWHAAGQIHTISSDPNISRMQGYTIRLYHEIAATSGVDIGLHATGGFY
                     LASNNVWYEYLKRERSKARYMGLDQEFISPAEVARRHPLIDPKHYIAALWDPLDGDID
                     PAGVVHAYAKSARHHGARYFTHTPVSATRQRADGTWDVETSRGTVNAEHLVNAAGLWA
                     REVGLMAGLHLPVQPMEHHYIITEAIPAIAERMEHGERLPAGIDYEANIYFRQERTGM
                     LLGTYEPRATPWKVAGTPRDFGHELLAPDLERIADRLQLAFERIPALGEVGVKTVING
                     PFTFGPDGNPMIGPVPGRRNYWVAVGVMAGFCQAGGVGLCLAEWMIEGAPSIDVWAMD
                     IARFGRFATPEWGTIKSTENYERRFVMTCPNETLPKGRRQKTTSLYDRLLERGAVMGV
                     SFGLEHALWFAKGREDAHEEPSFGRNRSHPYVGGEVAAVRSAAGVIEIANYGKHEIKG
                     RDARSFLSRLLAGRLPVPGRVALSPMLLPDGRLYGDLTVTCLNDEHFLLLGSGATQDM
                     HRRWFEAHVEAERVEYTNISDRLHGFAISGPRSRELLSKLCREDVSAQSLKFRDARST
                     FVGGVPAYLLRLAFTGELGYEIYCEPQFHLALWEQIESQGADLGLVPFGARALMSLRL
                     EKLWGVWTLDYRPDYTPAESGLDAFIDWEREFIGREEALKARAAGPPRRLVGLLVDVE
                     DRDVVGDEAILSGETCVGHVTSGGYAHHIGRPMAMGYVNAEHARDGTALIIEVNGELR
                     PARVVTRAPYDPDGRRMRG"
     gene            complement(5536..7056)
                     /locus_tag="VFB37_10935"
     CDS             complement(5536..7056)
                     /locus_tag="VFB37_10935"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZO23005.1"
                     /translation="MEREASRDRRRRRPERAPKAAVPYVTRRIPYYAFLDEEALTHIE
                     RQAFRLLQETGLEFREAPAALARWKNRGAEVRDTRVRIPEGLVRELLASAPAEFTQRA
                     RNPAHSVRIGGSAQVFAPVYGPPFVRDLKGGRRYGTLEDFHRLVKLTQRLDPLHHSGF
                     VTCEPCDVPVSKRHLDMLLAHMTLTDKPHLGAITARSRAEDSIEMARLLHGAAVLESE
                     CVIMANVNTNSPLLVDKVVTDAAEVYCGAGQGLIVVPFILSGAMGPVTTAAAIAQALA
                     EAMAVGAYTQLVRPGAPFILGNFLSSMNLRSGAPTFGMPEPLMSNLAIGQLARRLKLP
                     LRCGGALTASKLPDAQAAYESADSMLSTVLGGAHFVLHAAGWLEGGLVTGYEKLVLDA
                     DRLGAYAVMLQGFPTDDDAVGRDAYADVEPGGHFLGSSHTLAHYTTAFYEAALSDSNS
                     VEQWEELGSKDAAQRAFDRWQRLLAEYEPPPIDPAVRDSLEDYVARRKAELPDAWY"
     gene            complement(7081..8127)
                     /locus_tag="VFB37_10940"
     CDS             complement(7081..8127)
                     /locus_tag="VFB37_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZO23006.1"
                     /translation="MAVTTMAGAGSSRFEIAERPVVGPPELQCGPHADAVHSPFASRY
                     LAYRYPLRFASEDAGNRCDTYYLSPWMVMAVVDVASSERFESPLQGQDIVELHYRVSG
                     AIEIEGSWGECRFSGPAWLLWFQPQGCDDASEQLGEMRARETWVSLYCDRGWLRRVAE
                     RAASHLDLALEDAAEGRATPRFRVGPHLAATVPLLRDILGANREDPLHWLYASAKANE
                     LLYATLSHMPGYTPEVVPCRLTLRDRRQLQSVRDLLASDFASPPRLPALARQAGMNYS
                     KLCSGFRQLFGETTTEFVRRRRLEMAHELLRTTDLQVQQIARRVGYAHHGSFTAAFVH
                     HFGCSPKSVSRTPR"
     gene            8346..9536
                     /locus_tag="VFB37_10945"
     CDS             8346..9536
                     /locus_tag="VFB37_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO23007.1"
                     /translation="MAKSDTEAFPSLERLTLVRRPIGSARGLPNLTYTSPEYFRHERD
                     ELLGRTWAGLLFTDVIPERPYALPIDFLGLPLLVLRDRHGTLRVFHNVCSHRGMKLVA
                     DPGPLPGLITCRYHCWAYAANGDLKATPHFGGVNQPHAEGFDPAAHGLKPIRSALFMG
                     MLFINLSGDAPEFGRHSAPLTERAAELIGTDGWSQLHPGVSDSSLTLLAQCNWKLAVE
                     NYCEAYHLPWVHPELNSYSRLEDHYCFLGEGFAGQGSVTYRGSDVAGARLPRFPSWPA
                     DRQHVAEYPTMYPNVLLGFQADHAFAILLRPEAPDRTREELRIFYVGDAAAAESAGPS
                     RSAILSAWRIVFEQDITAVEQLQAGRASPGFDGGLFSPEMDAVTHHFHAWVADRLIEH
                     TPRG"
     gene            complement(9538..11010)
                     /locus_tag="VFB37_10950"
     CDS             complement(9538..11010)
                     /locus_tag="VFB37_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HZO23008.1"
                     /translation="MSRAKSVERTILGQPRGLATLFLTEMWERFSYYGMRAVLILYAV
                     AAVGEGGLGFDDRVASAVYGLYISATYVFALFGGWMADRLLGAQRAVIVGGVLIMGGN
                     AMLVLGHLQSFFLGLAVIVLGVGLLKPNISVMVAGLYAEGGARRDAGFSIFYVGISVG
                     ALLGSLLVPLCVTRLGWHWGFGLPVIGMLLGLAQFLSTRHWLGGAGLAGSPPRSWRAW
                     IPPLALVAGLVVLAAFLLTGTLRIDPVALSVLASWLIGLFTLGYFAYLLFFAGLDLAE
                     RRRVLVMAALFAAYAIFFAGFEQGGASLNLFAERYTERHVFGWDMPAGVLQGTTALYT
                     ILFAPAFAALWLALGRRALDPAPSIKFAAGLFLLAAGNLVIYFASRYVVSGVKVLPTW
                     LLLTYFLQECGDLCLSPVGLSSMTKLAPPRFAGQVMGLWFLALALGNNLAGQLSGEYD
                     ARNLESLPALFMKITLLSVIAGAVMLVLTPQLKRLMGGVK"
     gene            complement(11007..13010)
                     /locus_tag="VFB37_10955"
     CDS             complement(11007..13010)
                     /locus_tag="VFB37_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZO23009.1"
                     /translation="MPVEVKEHIWIPLSDGCRLGARIWLPEAAAHEKVPAILEYIPYR
                     KRDGTRGRDDPMHGYFAQCGYAAVRVDMRGSGESDGLLADEYLKQEQDDALEVIEWIA
                     RQSWCSGAVGMQGKSWGGFNALQIAARRPPALKAIITTFSTDNRYSDDVHYMGGCLLN
                     DNLWWGSIMLAYQSRPLDPLIVGPDWRARWLERIEALPFFPGLWLEHQRYDAYWKHGS
                     VCEDFGAIACPVLAIGGWTDSYTSAVLRLLEGLKVPRLGIIGPWGHVYPHDGTPGPAI
                     GYLQEAVRWWGHWLKGRDTGIMREPMLRAYLEDPVPPTGTRTSAPGRWVGERSYPSPD
                     IRSRGLHLRADRSLGESPGTAARFTVRSPQSHGKASGEWMGTGCPGEQATDQRLDDGG
                     ALVFDTATLNEEVAVLGTPVLRLRVAADVPVAQLAVRLSDVAPDEQVTRVSYQLLNLT
                     HREGDEQPAPLEPGRLYDVAVALKACGHRFVAGHRIRLAIATAYWPLAWPAPYPATLT
                     VVTEGSRLELPVRSSAADKAIHFPPPVHGPHSPVKVLVPSVVRRFSTQDHVTGETTYV
                     TEGIGGVFGEGILRFEAVDVELAHSLKRELTIRDADPLSARYVLTQSYEMGREGWRTR
                     IETRAQMHSDRTHFFLRGHLSALLNGEVVAERDWDLTIARDYL"
     gene            complement(13033..13572)
                     /locus_tag="VFB37_10960"
     CDS             complement(13033..13572)
                     /locus_tag="VFB37_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23010.1"
                     /translation="MVRHCVLWIALAPLMSASAAPLEQSTAPATCDSAQHRQMDFWIG
                     DWQVFDAQTRQLVGFDQVDKQYEGCVVRERLTFLTDMYRRPGISVRLAGIAINRFDGE
                     SWLQMWADNQWGAIVLRGRAIGGGKMEFVSIVPSRERDVKLVYEPQSDGTLRILQYVS
                     PAGRGKWTQYGDLEYRRNR"
     gene            complement(13605..16043)
                     /locus_tag="VFB37_10965"
     CDS             complement(13605..16043)
                     /locus_tag="VFB37_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZO23011.1"
                     /translation="MRVAVCIALYGVWPAAQAQQAAPADEATPSAIAEVTVTATRRTQ
                     TLEAVPYSLSVVTADQLAETGVTDIASLASQVPGLSTYEYGARFSAATVPIIRGINAT
                     GEPTRGFRTFEQAPVGTYIGNSPIEGYFQLDDLQRVEVLRGPQGTLYGAGTLAGALRL
                     IPNSPELNHWSGLVEGSAGRLDHSDGTPYTVRGLINVPLGDTLAFRAAAKYYYEPGFV
                     DTYGLFARTSNGLSGIPLLANSADPVTSPAIYRDQKDWNFQKTFTGRASVLWKPTDAF
                     SAEAAILDSSSHGDGGPQVNLDFRGGPSPLDPASTLPAGGPYREFSQIDQPWSRYTNL
                     ASLDLSYDAGFATVASTSSYYTSTGSTLQDNTYSLAGLASGGYLPYYAGVPTNPRFIY
                     DQTFTDRSHTFSQEVRLVSNTSPDKLFDYVVGFFYEDQTRAGAWTIAVPGTPEYQVAQ
                     GCLPNCFFDVGTAPGDVTFSQIDTQHFKDVSEFGELTWHFLQHGQVTFGARHFGQNFT
                     DAQSYDDYNFPTHLPAAPHESKASKTVGKVNPSFEYAPHQFVYAAWSQGFRRGGANSV
                     PLVGPFRESPLLATYTPDKTNNYEAGLKGRFDNGTSYTLALFDIKWDNPQISSSLPSG
                     NLAVYNGNTAESRGFEIESSGPLFLPRLTYSVSYAYADAHLTSSFSLPANDGTQTGTI
                     VPGLIHGSNGQQMPGSPKSSASLVLNYDMPLTAGYDLALAVNGVYRSAVPMQLTPSLG
                     VTTVQYSSTYEIMNLSATVNHEQWRGVLYITNVLDRQNILVPPTQFNELDKLTNDYIV
                     SPPREIGIRLGYKF"
     gene            complement(16339..18705)
                     /locus_tag="VFB37_10970"
     CDS             complement(16339..18705)
                     /locus_tag="VFB37_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZO23012.1"
                     /translation="MPSPHCARFALTVLCAFSSGLWAAESAPTAEDYARAERFMAYNV
                     APRVLNATGPAYWVQGGDFWYRRTTTAGSEFRWVDPAHPSSRPAFDHLQVAQALSAVA
                     AVPVEATSLPFHRFELSAGAIRFEAFAHRWSCELDRPRCTKLGPVDSSGALSPDGKQI
                     AFIRGDNLWVREVASGQESPLTIDGVKDFGYATDNSGWNHSDRAIVSWSPDSRRLATY
                     QQDQRQVGEMYLVRTMRGHPQLSAWKYAMPGDEAVPMLHRVVIDVASRTVVRLEMPPE
                     QRRTTHCWDEICQNGRMPDLQWSPDASELAFVSMSRDHKRVTVRVADTVSGTVRDVLT
                     EEVPTFYHGATGWAEDAENWRYLWASKELIWYSQRDNWGHLYLYDAASGRLKRQISRG
                     AWNVIALMGVDGKRRAAYALGAGREVGRDPYFAHLYRLPLDGGEAQLLTPEKATHVIS
                     MDPSLEYFVDRYSTPDTPPVTVLRDRHGRLLKVLERADIEGLKAAGWRPPLPFTVKGR
                     DGRTDVYGMLFLPSHFDTQRRYPIINSIYPGPQIGSVGPRTFLRAGVLGDEQALAELG
                     FAVVQIDGMGTSMRSKAFQDTNYGNLCENTLSDQVAGMRELAQRDSWIDLQRAGIYGI
                     SGGGWASAAALFRYPEFFKVGIAMSGVHDVLGYEGDWGEEFMGLLVRHADGTTSYDGS
                     VNTLSAGKLAGHLLLMHGTMDDNVPPYQTLQLVDALIKANKDFDLILLPNQRHEPVGV
                     AAAYATRRRWDYFVRYLLQTEPPHGVALQPASDASLQSLARASSEAEF"
     gene            18977..20395
                     /locus_tag="VFB37_10975"
     CDS             18977..20395
                     /locus_tag="VFB37_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO23013.1"
                     /translation="MQAGRPAEAVVFAERAVAGETTCVSAHGLLATLLLQVGRAKEAD
                     EMVTHALQCSPGSADAYDALAHVSMLLGKHERANLLYRRVVKLAPDDARFWYNLASSE
                     RSFGRLQEAESACNRAIALDSSHYASYLLRSELRVQSAENNHLGELQSALSRPGLDER
                     ARVPLGYALAKELDDLQRYSEAFQYFQESAQIRRRHLSYSIAMDEHKIARIQQIFTPD
                     LIQRRARPEGSSRFIFIVGLPRSGTTLLEHILSGLERVRSNGETDYFARALFAAAPAG
                     PQDVFARAALADPGRVAASYGHFADHDSNGTRIIEKLPLNYLYLGAIHRALPQAKLVL
                     VRRSPIDACFAMYRTLFGVGYPFSYDFEDLARYHAAYDQLLEHWRKTLKEELCEVVYE
                     ELVSDPLQTGARVAQHCGLTWNPRAIEVEKSATISMTASAAQVRRPIYGSSSGRWRHY
                     IQHLGPLLEALRRHRVISADSV"
     gene            complement(20415..20549)
                     /locus_tag="VFB37_10980"
     CDS             complement(20415..20549)
                     /locus_tag="VFB37_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23014.1"
                     /translation="MIHEFAALLTDQVSQSIPVLPLEARDAKQQRLSGQRFEGREWAL
                     "
     gene            21046..22644
                     /locus_tag="VFB37_10985"
     CDS             21046..22644
                     /locus_tag="VFB37_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZO23015.1"
                     /translation="MSTTWWQRGVIYQIYPRSFQDSNSDGVGDLHGIRARLRYCAGLG
                     VDALWLSPIYPSPMTDFGYDICDYTGIDPVFGTQTQFDELIAEVKQLGMKLILDYVPN
                     HTSDQHPWFKESRSSRTNPKRDWYLWRDRAPGGGPPNNWLSQFGGSAWQWDTVTAQYY
                     YHAFLQTQPDLNWRNPQVRAAMYDVLRFWLRRGVDGFRVDVLWHLIKDDQWRDNPPNP
                     RYLPGEPPQHSQIPLYNTDRPEVQQVVVEMRKVVDEFPDRVLIGEIYLPLERLVAYYG
                     AQLDGVNLPFNFHLLLTAWNARAIATWIERYERALPPGGWPNWVLGNHDNPRIASRVG
                     ILQARVAAMLLLTLRGTPTMYYGDEIGMPNTPIPPDRVQDPYEKNVPGIGVGRDPSRT
                     PMQWTASANAGFSDHEPWLPIAADYEVVNVHTEDADPRSILVLYRRLLALRRSHPALI
                     EGAYEPVTMTGDLLAYVRHSPRGRLLIALNLGAGPFDLLLGSQGMRGRILLSTHLDRE
                     TGPELADLALRANEGVILELARDQ"
     gene            22634..24340
                     /locus_tag="VFB37_10990"
     CDS             22634..24340
                     /locus_tag="VFB37_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HZO23016.1"
                     /translation="MINDLWYKNAVFYCLSVGTYMDANGDGIGDFEGVARRLDYLQGL
                     GITALWLMPFQTSPGRDDGYDVSNYYEVDPRYGTLGDFVEFTHAAAQRGIRVIIDLVV
                     NHTSDQHPWFQEARRDPHSKYRDWYVWSRKRPPDSSKGVVFPGVQKSTWSYDSVARAW
                     YFHRFYDFQPDLNTANPQVLSEILKIMGFWIQAGVSGFRMDAVPFVIATKGPAVRRPL
                     EQYEMLRAIREFLQWRKGDAIVLAEANVLPDTDMEYFGRDGDRMHMMFNFQVNQNLFY
                     ALAAQDVRPLSVALRKTKPRPDTAQWGQFLRNHDELDLGRLTDPQRERVFREFGPEPS
                     MQLYRRGIRRRLAPMLAGDRRRIELAYSLMFTLPGTPVIRYGDELGMGDDLKLPERNC
                     ARTPMQWSTEPNAGFTKSDKPISPVISSGPYGYEHVNAAQQRRNPESLLNWTERIIRM
                     RKEVPEIGWGDFQLLPTGDVTVLAIRYDWRNNSVVFVHNLSGKPRELTFDVGLQSEDA
                     SLLINLLSDDHSRARHGRHHIVLEGYGYRWFRVGGLDYLLKRSNVEVQSKVHKIARRR
                     KR"
     gene            24368..24550
                     /locus_tag="VFB37_10995"
     CDS             24368..24550
                     /locus_tag="VFB37_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23017.1"
                     /translation="MSNGHETFAGVIRLSSSHTVPETLDRLERLLKEHGVTIFARIDF
                     SGDAARAGSPCHPSSS"
     gene            24529..24774
                     /locus_tag="VFB37_11000"
     CDS             24529..24774
                     /locus_tag="VFB37_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZO23018.1"
                     /translation="MPPEQQLIFGNPKAGTPLMVSQPTAALDLPIRAICWQDSLGKTW
                     IAYNAPEYVVQRHWLPAEFAKNLAAVIPLLERAAGRS"
     gene            complement(24771..25091)
                     /locus_tag="VFB37_11005"
     CDS             complement(24771..25091)
                     /locus_tag="VFB37_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23019.1"
                     /translation="MQYAQVIGIALLGAVVATGSLAASLRIELPKETATLKPGPGADL
                     TQAQCLICHSADYIITQPPDKPLAFWKAEVEKMKKVYGAPIPDDQIDSVAEYLARNYG
                     TAGT"
     gene            complement(25100..>25830)
                     /locus_tag="VFB37_11010"
     CDS             complement(25100..>25830)
                     /locus_tag="VFB37_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZO23020.1"
                     /translation="GVPLKTVLDKAGVQAGAKQVRFDGLDGPVLPATPDFAKALEVDH
                     AMDGEVMIAYSMNDADLPMLNGYPVRLVVPGYFGTYWVKHLNEITVLDSVLDNFWMTT
                     AYRIPDNACACVPPGTPSKKTVPISRFAIRSFVTNLKEGSTVRAGRPLVVRGIAFDGG
                     YGIREVDLSVDGGRTWRQTTLGKDLGKYSFRQWQTTVALERGAHAIQVRAVNVIGQSQ
                     PLEALWNPSGYMRNVVETTRVTAA"
BASE COUNT         4700 a         8473 c         8111 g         4546 t
ORIGIN      
        1 ggcctcggag agatatacca gcaaggtcgc gaaccacgcg acgaatccgc ctacgacccc
       61 cgccgcggcg cacagaagga ggtccgtaga cgtcagaacg ggcagtgcgt gctgcgtcgt
      121 gaagagcgcg ccgacgccaa gcaagcgcca gcgcaatgcg gtcgccacta ccacggcaat
      181 cgacatcggc acgaaggagc gcggtcggaa ttcaaacagg agcaattcca cggccagcag
      241 aatcgatgcc aagggtgcat tgaatgtcgc cgccatccca gctgcagcgc cggccacgag
      301 cagcgtcttg cgctcgtcag cggtcagcga gagggtctgc gccatcagcg atccaaaagc
      361 ccctccagtc atgatgatcg gaccttcggc gccaaacggg ccgccggtac cgatggaaat
      421 cgccgcagac acgggtttca acaaagcgac gcggggacgg atcttgctcc cgccgcgcat
      481 gatggcctcg atcgcctccg gcattccgtg cccacggatc gcctcggaac catatcgcgc
      541 catgaggccg actatgaggc cgcccaccac cggcgcgccg atgatcagcc accacggatg
      601 cacgccagat cccggcgcga cgaggccagt ggcgacccgc tggtagaaaa ccaaattcgt
      661 gatcagtccg atcagcttga gcaggccgtc ggcgacgagg gccgcgacgg cgccaaccgc
      721 cagcgcaagt gcgctgatga tgacgatgcg ggggttaacc gagtaatcgc cgagtcgcgc
      781 cggtactcgc aaaggagcgt gaccgctttg ttgttgcatg gaattccttc ttaaatgatt
      841 cagcgtatgc cctatgtgcc gtcgtctccg gacaccaaga tgcgcaccgg cgaatgccgc
      901 gctttgcgcg cgatctgcag cgcctcgcaa tcagaggaac cggccgagga gcgcgctcgg
      961 gaaacgttca agcactgaaa cgaccgcatc agcatcctga gctgcgccgc tcggccaaac
     1021 accaggttgt ccgctatctt ctgaatgtct cgatccaacc agcctcggct ttacacgtag
     1081 cggggaatgg gcccgacctg gggcgtggtg ttttcgccca gatcgatgaa aacttcatcg
     1141 atgtagcacc aaccccaccc ctccggcgga tcgtaccctt cgatgatcgg gtgccgcgtg
     1201 gcatggaagt gcttcgtggc gtgccgctcg ggggagtcat cacagcatcc gacatggccg
     1261 cacttgcgac agatccgcag atgaacccag cgcgtgccca tcttgaggca ctcctcgcac
     1321 cccttcgcgc tcggcttgac aaccacgatg gacttgaggt gagaacacga cttgctcatg
     1381 cgaaaccctt tgataaatgc ctgggatggc gcgaatgtcc ctctgtccgc tgctcagtca
     1441 cggcaggcag gtggcgaatc cctcctcagg agggtgatca taagcgatcc gcggacgaga
     1501 cccgaacgcc ggagttccgt cgcgtgcggt cacgaagatt tcggaaccca ctcgggccac
     1561 aatgacctga tacagtctgg aatacactga cttagctgtc atcgacatgt tttccgtaac
     1621 agcgttgctc gccggagcgt ggggacttgc gttcgtgttc ctcaatgtcc tgctcgagca
     1681 ggctggactt ccgatccccg ccgtaccgac cttggtgctg gccggcgcct tggctgccaa
     1741 gcggcctgaa tggggattag cggctttcgc actcgcgctc gcggcttgcg tcatcaccga
     1801 tgtcgcttgg tacggggctg gacgagccta cggcgggcgc atcgtgcgac tgctctgcag
     1861 gatctccctt tcacctgact cgtgtgtgag tgagacccag ctgagattcg agcgctgggg
     1921 cgcccgcgca cttctcctct ccaagttcgt gcccggtctc gcgaccttgg ccccctctct
     1981 gtctggcgcc ctcagaatgc aactgagctt attcgtcggg atgagcgcac tcggctccgc
     2041 attgtgggtc atcgccttca tggttctggg cacgctcgct gcgccacaga tcacggctct
     2101 gctcccgcag gttgcagccc tcgggggacg agctgccatc ctgatcgccc ttctgctggt
     2161 tctctatatc ctcttcaagt gggtgcagcg ccagcgcgtc tatgcggagc ttcgcatggc
     2221 tcgcgtcgag gtgggcgaac tctatttaat gctccagcgt gtaccggcgc cgaccattct
     2281 cgatgtgaga tccatgagtg cccacgcgct cgatccccgc gcaatcccag gtgctgtgca
     2341 cgtgccgccc gaggacatcg cggcccgcat cgccgctgtc gaggccaacg ccgaggttgt
     2401 tgtctactgc aattgcccga atgaagcctc cgccgcgcgt gtcgctcaac tgctgagacg
     2461 gcacggtgtt gtccgcgtgc gcccccttct gggcggcttg gatgcgtggg tagcggcagg
     2521 ctatctggtg accgatacga gatcagcgcc tgcggcacca gcaccaagtt tggcaagctc
     2581 tcgaccggcc gcagccaccg acttgccaaa agaacctgag gcgccctccc cgcggcaacg
     2641 ctcatcatca tgaccaacca tccggcgagt acggcgccgt agcaccatcg cacctgtccc
     2701 ttgatgtcat ccctgatagt gggttgcatg agcgcgcgga tcctgcctcg caagcctgag
     2761 cagggccctg gaacggtttc acagatgcgg gcgtttcacg gaccgacagt cgtaagcctt
     2821 cgtggagcac gaaagactac cgcgcaaggc tcggaccggg gaaaacgcga gcaccgccat
     2881 gatcgcgtcc gatcgtcacc aggcgcggca gctcaggagt tacgtgccgc gatcgagtat
     2941 ggatgcactc tgccagggct ggccggcgcc ctcgcgctgt tcgctttgct ggccaccaca
     3001 atgaccttga cccgcaaggt gggctggtac gccgaggagg cctaggggtc cgctgctcgc
     3061 gaccctgcct cacccccgca tgcgtcgacc atccggatcg taaggcgccc gggtcaccac
     3121 acgcgcaggg cgaagctcgc cattgacctc aatgatcagt gcagtcccgt cgcgggcgtg
     3181 ctctgcgttc acgtagccca tcgccatcgg gcggccgatg tgatgcgcat agcctcccga
     3241 ggtgacatgt ccaacgcaag tctcgcccga aaggatggcc tcatccccca ccacatctcg
     3301 gtcctcgacg tccaccagca accccaccaa gcggcgaggc ggacccgcgg cccgcgcctt
     3361 cagcgcctcc tcgcgcccga taaattcccg ctcccaatcg atgaaggcat ccagtccgct
     3421 ctcggccggc gtgtagtcgg gccgatagtc gagggtccac actccccaga gcttctcgag
     3481 gcgcaggctc atcagcgccc gtgccccgaa gggcacgagc cccagatcgg cgccctgcga
     3541 ctcgatctgc tcccagagcg ccagatgaaa ttgaggttcg cagtagatct cgtagccgag
     3601 ctcgccggtg aaggcaaggc gcagcaagta tgcgggcacg cctccgacga aagtcgaacg
     3661 cgcatcgcga aacttcaggc tttgagcgga cacgtcctcg cgacagagct tcgaaagcag
     3721 ctcccgggag cgaggtcccg agatcgcaaa accgtgcagc cgatccgaga tgtttgtgta
     3781 ctcgacacgc tcagcctcga cgtgcgcctc gaaccagcgc cggtgcatgt cctgggtcgc
     3841 accggatccg agcaacagga aatgctcatc gttcaggcac gtcaccgtga gatcgccata
     3901 gaggcgcccg tcagggagca acatcggact caaggccaca cggcccggca cgggcaaccg
     3961 ccccgcgagc agccgactga ggaaagagcg cgcatctctt cccttgattt cgtgcttgcc
     4021 atagttggcg atctcgatca cgcccgccgc gctgcgcacc gccgcgacct cacccccgac
     4081 atacgggtgg ctgcggttgc ggccgaagct cggctcctcg tgcgcgtcct ctcgaccctt
     4141 agcgaaccac aacgcgtgct cgagaccgaa ggacactccc atgacggctc cgcgctcgag
     4201 cagccggtca tagagggacg tggtcttctg ccgtcgcccc ttcggcaacg tctcgttcgg
     4261 gcaggtcatc acgaagcggc gctcatagtt ctcggtggac ttgatcgtgc cccactccgg
     4321 ggtcgcgaag cgcccgaacc gggcgatgtc catagcccac acgtcaatcg agggcgcgcc
     4381 ctcgatcatc cactccgcca gacacagacc gacgcccccg gcctggcaaa agcccgccat
     4441 gacgccgacc gccacccagt aatttcgccg tcccggcacc ggcccgatca tcggattgcc
     4501 gtccggacca aaggtgaagg gaccgttgat cacggtcttg accccgactt cgcccagggc
     4561 cgggatgcgc tcgaaggcga gctgcagccg gtccgcgata cgctccagat ccggtgccaa
     4621 caattcgtgc ccgaagtccc gcggcgtgcc ggccaccttc cagggtgtcg ctcgcggctc
     4681 gtacgtgccg aggagcatgc cggtgcgctc ctgcctgaag tagatgttgg cctcgtagtc
     4741 aatacccgcc ggcaggcgct cgccgtgctc catccgctcg gcgatagctg ggatcgcctc
     4801 cgtgatgatg tagtgatgct ccatcggttg taccggcaag tgcagccccg ccatcagtcc
     4861 cacttctcgt gcccagagcc cagcggcatt gacgagatgt tcggcgttca ccgtcccgcg
     4921 cgatgtctcg acgtcccatg tcccatccgc ccgttgtcgc gtcgcgctta ccggcgtgtg
     4981 cgtgaaatag cgagcgccgt ggtggcgggc cgatttcgcg taggcgtgca ccacgccggc
     5041 cgggtcgatg tctccatcga gtggatccca cagcgcggcg atatagtgtt tggggtcgat
     5101 cagcggatgg cgtcgggcga cctcggccgg gctgatgaac tcctgatcga ggcccatgta
     5161 gcgcgccttc gagcgctcgc gtttcaggta ctcgtaccag acattgtttg aagccaggta
     5221 gaacccaccg gtcgcgtgca gcccgatgtc cacgccggaa gtggccgcga tctcgtgata
     5281 caggcggatc gtgtaaccct gcatccggct gatgttcggg tcggagctga tcgtgtgaat
     5341 ctgcccggcg gcgtgccagc tcgagccgct ggtgagctcc tggcgctcga gcaggatgca
     5401 gtcacgccag ccgtgcttgg ccagatggaa caggatggag cagcccacca cgccaccccc
     5461 gatcacgacg acccttgcgt gagacggcag agacgtggca tctgagctcg caggctcgct
     5521 catgcgcagc ttcctctagt accaggcgtc gggcagttcg gccttgcgcc gcgccacgta
     5581 gtcttcgagc gagtcccgga ccgccggatc gatcggtggc ggctcgtact cggcgagcag
     5641 acgctgccag cggtcgaagg cccgctgtgc cgcgtccttc gaacccagct cctcccactg
     5701 ctcgacactg ttggaatcgg acagcgcggc ttcatagaag gcggtcgtgt agtgcgcgag
     5761 cgtgtgagat gagcccagga agtggccgcc cggttccaca tctgcgtaag catcgcgccc
     5821 cacggcatca tcatcggtgg gaaagccttg cagcatcacc gcgtaggccc ccagtcgatc
     5881 cgcatccaac acgagcttct cgtagccggt gacgaggccg ccctcgagcc aaccggccgc
     5941 gtgcagaacg aagtgcgccc cgccgagcac cgtggagagc atcgagtcag cgctctcgta
     6001 ggcagcctgc gcatccggga gcttcgatgc ggtcagcgcg cccccgcagc gcagcggcag
     6061 cttcaggcgg cgcgcgagct ggccgatggc aaggttggac atgagcggct cgggcatgcc
     6121 gaaggtcggg gccccggagc gcaggttcat ggaggacagg aagttgccca ggatgaaggg
     6181 tgcaccggga cgcacgagct gcgtgtaggc gcctacggcc atcgcctcag cgagcgcctg
     6241 ggcgatggct gccgccgtcg tcaccggccc catcgccccg gagaggatga agggcaccac
     6301 gatcagacct tggccggcac cgcagtagac ttccgcggcg tccgtcacga ccttgtcaac
     6361 cagcagcggc gagttggtgt tgacattggc catgatcacg cattcggact cgaggaccgc
     6421 tgccccgtgc agcagtcgtg ccatctcgat actgtcctcg gcacggctgc gggcggtgat
     6481 cgcacccagg tgaggcttat cggtcagcgt catgtgggcg agcagcatgt ccaaatgccg
     6541 cttggagacc ggcacgtcgc agggctcgca ggtgacgaag cccgagtggt gcagcggatc
     6601 cagtcgctgc gtgagcttca ccaggcgatg aaaatcctcg agcgtcccgt aacggcgccc
     6661 ccctttgaga tcccgcacga agggcggccc gtagaccggt gcgaacacct gggcagagcc
     6721 gccgatccgc accgagtgcg ccgggttgcg cgcccgttgc gtgaactccg cgggtgcgct
     6781 cgcgaggagc tcacgcacca gaccttcggg aatacgcacg cgcgtgtcgc gcacctctgc
     6841 gccacggttc ttccagcgcg ccagcgccgc cggagcctcg cgaaactcga gtcctgtctc
     6901 ctgaagcagc ctgaaggcct ggcgctcgat gtgggtcaac gcctcctcat cgaggaatgc
     6961 gtagtaagga attcggcggg tcacataggg caccgccgcc ttgggtgccc gctcgggccg
     7021 ccgccgccgt cgatcacggg aagcttcacg ctccatgtgc ggcgctcatc gcatgctcgc
     7081 ctaacgcggc gtccggctga cgctcttcgg tgagcagccg aagtggtgga cgaaagccgc
     7141 cgtgaagctg ccatgatgtg cataccccac ccgacgcgcg atctgttgca cttgcaggtc
     7201 cgtggtgcgc agcagctcgt gcgccatctc caggcgccgg cgtcgcacga actcggtcgt
     7261 ggtctcaccg aagagctgtc ggaacccact gcagagcttc gagtagttca tgccggcctg
     7321 tcgtgcgagg gccggtaacc gcggtgggga ggcaaagtcg gacgcgagca gatcgcgcac
     7381 cgactgcagc tgcctgcgat cgcgcagcgt gagccggcag gggacaacct ccggcgtgta
     7441 cccgggcatg tgactcaacg tcgcgtagag cagctcgttc gccttggcgc tggcatagag
     7501 ccagtgcagc ggatcctcgc ggttcgcgcc cagaatgtcc cggagcagcg gaacggttgc
     7561 ggcgaggtgg gggccgacgc gaaagcgcgg cgtggcgcgc ccctcagcgg catcctccag
     7621 cgcaagatcg aggtgcgagg cggctcgctc ggcgacccgg cgcagccaac cgcgatcgca
     7681 atagaggctg acccaggtct cgcgggcccg catctcaccc aactgctcgc tcgcatcgtc
     7741 acagccctgc ggctggaacc acagcagcca ggcaggcccg ctgaagcggc actctcccca
     7801 ggagccttcg atctcgatcg ctcccgagac ccggtagtgg agctcgacga tgtcctggcc
     7861 ctgaagcggg ctctcaaagc gctcgctgga ggccacatcg acgaccgcca tcaccatcca
     7921 gggcgagaga tagtaggtgt cgcagcggtt gcctgcgtct tcagaggcga agcgcagcgg
     7981 ataccggtaa gcgaggtagc gcgaggcgaa cggcgaatgc accgcatcgg catggggacc
     8041 gcattgcagc tccggtggtc cgaccacggg tcgctcggcg atctcgaatc tgctgctgcc
     8101 agcgcccgcc atcgtggtca cggccatagc accccctcgc atcgctcggc ctcgcacgac
     8161 catgccgcgc cctgcccgtg ctgtcaacgc tcagctcccg tgtcacaacg actgagctgc
     8221 agcgtgcgcc gcctttctta gtcgcacatc agagtctctt aggagccctg aattaccggc
     8281 gcgaggggcg gcgacctccc cggcgaatgc acctgtgagg tgataggctg ctctctgcaa
     8341 gccgcatggc aaaatccgac accgaagcct ttccctcctt ggagcgcctc acgctcgtgc
     8401 ggcgaccgat aggctcggcc cggggcctgc ccaacctgac ctatacgagc cctgaatact
     8461 tccgccacga gcgcgatgag ctgctcgggc gcacctgggc cggcctgctc ttcacggatg
     8521 tcattccaga gcggccttat gcgctaccga tcgacttcct cggcctgccg ctcctggtgc
     8581 tgcgcgaccg gcacggcacc ttgcgcgtat tccacaatgt ctgcagccat cgcggcatga
     8641 agctcgtagc cgacccgggc cccctgcccg gcctcatcac ctgtcgctat cactgctggg
     8701 cgtacgcagc gaacggcgat ctcaaggcca ccccccactt cggcggcgtc aaccagcccc
     8761 acgcagaagg gttcgatccg gccgcgcacg gtctgaaacc catccgcagc gccctcttca
     8821 tgggcatgtt gttcatcaat ctcagtggcg atgcgcccga gttcgggcgg cactcggctc
     8881 ctctcaccga gcgcgctgcc gagctcatcg gcacggacgg ctggtcgcag cttcaccccg
     8941 gggtgagcga ctcgagcctg accctgctcg cccagtgcaa ctggaagctc gcggtcgaga
     9001 actactgcga ggcctaccac ctgccctggg tacatccgga gctgaacagc tattcgcgtc
     9061 tcgaggatca ctattgcttc ctcggtgagg gcttcgccgg tcagggatcc gtcacttatc
     9121 gcggcagtga cgtcgcgggc gcgcggctgc cgcgcttccc atcgtggccg gccgatcgcc
     9181 agcacgttgc cgaatatccg acgatgtatc ccaacgtctt gctcggcttc caggccgatc
     9241 acgccttcgc catcctcctg aggcctgaag ctccggaccg cacgcgcgag gagctgcgga
     9301 ttttctacgt cggcgacgcg gcggccgcgg agagcgccgg cccctcacgc tcggccatcc
     9361 tgtccgcctg gcgcatcgtc ttcgaacagg acatcacggc cgtggagcag ctgcaggcgg
     9421 ggagagcctc gccaggcttc gacggtgggc tgttctcccc ggagatggat gccgtcaccc
     9481 atcacttcca cgcctgggtg gcggatcgcc tcatcgagca cacgccccgc ggctagttta
     9541 tttgacgccg cccatcaggc gtttgagttg gggcgtgagc acgagcatca ccgctcctgc
     9601 gatcaccgag agcagggtga tcttcatgaa gagcgccggc agggactcga gattgcgggc
     9661 gtcatattcg ccggacagct ggccggcgag gttgttgccg agcgcgagcg cgaggaacca
     9721 cagccccatg acctggccgg cgaagcgcgg cggggcgagc ttggtcatcg aggacaaccc
     9781 gaccggcgag agacagagat caccgcactc ctgcaggaaa taggtgagca gcagccaggt
     9841 cggcaggacc ttcacgccgg agaccacata ccgcgaggca aagtagatca cgaggttgcc
     9901 ggctgcgagc aggaacagtc ccgccgcgaa cttgatcgac ggtgccggat ccagagcccg
     9961 ccggccgagt gccagccaca gcgccgcgaa agccggcgcg aacagaatgg tgtacagcgc
    10021 cgtcgtcccc tgcaacaccc cggcaggcat gtcccagcca aagacgtgcc gctcggtata
    10081 gcgctcggcg aagaggttca gtgaggcccc gccctgctcg aaccctgcga aaaagatggc
    10141 gtacgcggca aacagcgctg ccatgaccaa cactcggcgt cgctccgcga ggtccaggcc
    10201 cgcgaagaac agcagatagg cgaagtagcc gagcgtgaac agcccgatga gccaggaggc
    10261 gagcaccgaa agggcaaccg gatcgatccg caacgtgccg gtgagcagga atgcggccag
    10321 taccacgagc cctgcgacga gcgcaagcgg gggaatccag gcgcgccacg accgcggcgg
    10381 actgcccgcc agaccggccc cgccgagcca gtgccgcgtc gacaggaact gcgcaagtcc
    10441 cagcagcatg cctatcaccg gcaggccgaa gccccaatgc cacccgaggc gcgtcacgca
    10501 caacggcacg agcagcgatc cgagcagcgc gccgacgctg atgccgacat agaaaatgga
    10561 gaacccggcg tcccggcgcg cacccccttc cgcgtacagc cccgctacca tgacactgat
    10621 gttgggtttg agcagcccga ctccgaggac gatgacggcg aggcccagga agaaggactg
    10681 cagatgcccg agcacgagca tcgcattgcc gcccatgatc agcacgccgc caacgatcac
    10741 ggcgcgctgc gcaccgagca gccggtctgc catccagccc ccgaacagag caaacacata
    10801 agtggccgag atgtagagac catagaccgc actcgccacg cgatcgtcga atccgagacc
    10861 gccttccccg accgcggcga cggcatagag aatcagcacc gcgcgcatcc cgtagtacga
    10921 gaagcgctcc cacatctcag tcaggaatag tgtcgcaagc ccgcgaggct gcccgagtat
    10981 cgtccgctcc acggatttcg cccggctcac aggtaatcgc gtgcgatcgt gaggtcccag
    11041 tctcgttccg cgaccacttc gccgttgagt agcgcgctca gatggccgcg aagaaaaaag
    11101 tgcgtgcgat cgctatgcat ctgcgcccgc gtctcgatgc gcgtccgcca gccctcccgg
    11161 cccatctcgt aggactgagt cagtacgtag cgcgccgaca gtggatccgc atcccgaatc
    11221 gtcagctcgc gcttgagact gtgcgcaagc tccacatcga ctgcctcgaa ccgcaggatc
    11281 ccctccccga agacaccgcc gatgccttcg gtcacgtagg tcgtctcacc ggtgacgtgg
    11341 tcctgggtcg agaagcggcg cacgacgctg ggcacgagga ccttgacggg cgaatgcggc
    11401 ccgtgcaccg gtggtgggaa gtggatcgct ttgtccgcag cgctcgagcg caccggcagc
    11461 tcgagccgac tgccctcggt aaccaccgtg agtgtggcgg ggtatggcgc gggccaggca
    11521 aggggccagt aggccgtcgc aatggcgagc cggatccgat gccccgcgac aaagcggtgc
    11581 ccgcaagctt tcaaggcgac cgcgacatcg tagagccggc ccggctcgag cggtgcgggc
    11641 tgctcgtctc cttctcgatg cgtgaggttc aggagttgat agctgactcg tgtgacctgc
    11701 tcatctggtg ccacgtcgga cagccgcacc gcgagttgcg cgaccgggac gtccgctgcc
    11761 acccgaagcc gcagcaccgg tgtgccgagc accgcgacct cctcattcaa agtcgcggtg
    11821 tcgaagacga gggcccctcc gtcatcgagg cgctgatcgg tcgcctgctc acccgggcaa
    11881 ccggtgccca tccactcccc gctcgccttg ccgtgacttt gcggcgagcg caccgtaaaa
    11941 cgcgccgccg tacccggaga ctccccgaga ctgcgatccg cgcgcaggtg caggccgcgt
    12001 gagcggatgt cgggcgaagg atacgagcgc tcgcccaccc aacgccccgg agcagacgtg
    12061 cgtgttccgg tgggcggcac ggggtcctcg aggtaagcac gcagcatggg ctcccgcatg
    12121 atcccggtgt cgcggccctt gagccaatgg ccccaccagc gcaccgcctc ctgcaggtaa
    12181 ccgatggccg gtccgggagt gccatcgtgc ggatagacgt ggccccaggg accgatgatc
    12241 ccgaggcgcg gcaccttgag cccctcgagc agccgcagca cggcactcgt gtagctgtcg
    12301 gtccaaccgc cgatcgccaa tacgggacac gcaatggcgc caaagtcctc gcagaccgag
    12361 ccatgcttcc aataagcgtc gtagcgctgg tgctcgagcc acagccccgg aaagaacggc
    12421 agcgcctcga tgcgctcgag ccagcgcgcg cgccagtccg gtccgacgat aaggggatcc
    12481 agcggtcggc tctggtatgc cagcatgatg ctgccccacc agaggttgtc attcagcaaa
    12541 cacccgccca tgtaatggac atcgtcgctg tagcgattgt cggttgaaaa agtggtgatg
    12601 atggccttca gggccggcgg gcgccgggcg gctatctgca gagcgttgaa gccgccccaa
    12661 gacttcccct gcatgccgac ggccccgctg caccaggact gccgggcgat ccactcgatc
    12721 acttcgagcg catcgtcctg ctcctgtttc agatactcat ctgcgagcag cccgtccgac
    12781 tcacctgagc cgcgcatgtc cacacgcacc gccgcatagc cgcactgggc gaagtagccg
    12841 tgcatggggt catctcggcc gcgcgtacca tcgcgcttgc ggtagggaat gtactcgagg
    12901 atcgcgggca ccttctcgtg agccgcagct tccggtagcc agatgcgcgc acccaggcga
    12961 cagccgtcgc tcaatgggat ccagatgtgt tccttgacct cgaccggcat gtcaatttct
    13021 gcaggacgcc ccctaccggt tgcgacggta ttcgagatcg ccgtactgcg tccactttcc
    13081 ccggccggcc ggcgagacgt attgcagaat gcgcagcgtt ccgtcggact gcggttcgta
    13141 cacgagcttc acatcgcgct cgcgggatgg aacgatcgag acgaactcca ttttcccacc
    13201 cccgatcgcc ctcccgcgca agacgatcgc cccccactgg ttatcggccc acatctgcaa
    13261 ccagctctcg ccgtcgaagc gattgatggc gatgccagcc aggcggacgg agataccggg
    13321 acggcggtac atgtcagtca ggaaggtcag cctctcccgg acgacgcatc cttcgtactg
    13381 tttatccacc tgatcgaagc cgaccagttg ccgggtctgt gcatcgaaga cctgccagtc
    13441 accgatccag aagtccatct gccggtgctg cgccgagtcg caggtcgcag gagcggtgct
    13501 ctgttccaac ggcgcagccg atgcgctcat caggggcgcc agcgcaatcc agagcacgca
    13561 gtgccgcacc attctcaatc cctcgaatgc aacccccgct gcgctcagaa tttgtagccc
    13621 aggcgaatac cgatctcccg cggtggactc acgatgtagt cgttggtcag cttgtcgagc
    13681 tcattgaact gcgtcggagg cacgaggata ttctgccggt cgagcacatt cgtgatgtaa
    13741 agcaccccgc gccactgctc gtggttcacg gttgccgaca gattcatgat ctcgtaggtc
    13801 gaggagtact gcaccgtcgt cacaccgagg gagggtgtca gctgcatcgg cacggcgctg
    13861 cggtacacgc cattgactgc gagcgcgagg tcatagcctg cggtcaacgg catgtcatag
    13921 ttgagcacaa ggctcgcact gctcttggga ctccccggca tctgctgtcc gttgctccca
    13981 tggatcaggc ccggaacgat ggtgcccgtc tgcgttccat cgttggcggg cagcgagaag
    14041 ctgctcgtca ggtgagcatc cgcgtacgca tagctcaccg agtaggtgag gcgcggcagg
    14101 aagagcggtc cgctcgattc gatctcgaag ccgcgcgatt cggccgtgtt gccgttgtac
    14161 acggcgagat tgcccgaagg cagactcgag gagatctggg ggttgtccca cttgatgtcg
    14221 aacagcgcga gcgtgtacga ggtgccgttg tcgaagcgac ccttgaggcc cgcttcgtag
    14281 ttgttcgtct tgtcgggcgt gtaggtcgcc agcagcggac tttcccggaa cggaccgacg
    14341 agtggcaccg agttcgcccc accgcggcgg aagccctgcg accacgcggc atacacgaac
    14401 tggtgcggcg catattcgaa ggagggattg accttgccga cggtcttgga ggccttcgac
    14461 tcgtgcgggg ccgccggcag atgcgtcggg aagttgtagt cgtcgtagga ctgagcgtcc
    14521 gtgaagttct gaccgaagtg ccgggctccg aaggtcacct ggccgtgctg caggaaatgc
    14581 caggtgagct ctccaaactc cgagacgtcc ttgaagtgct gggtgtcgat ctgactgaag
    14641 gtgacatcgc ccggggccgt gcccacatcg aagaagcaat tcggcagaca cccctgcgcc
    14701 acctggtatt ccggcgtacc ggggacagcg atcgtccacg cgcccgcgcg agtctggtct
    14761 tcgtaaaaga accccacgac atagtcaaag agcttgtccg ggctggtatt tgagaccaga
    14821 cgcacttcct gcgagaaggt gtgcgaccga tcggtgaagg tctgatcgta gatgaatcgc
    14881 gggttggtcg ggacaccggc gtagtacggc aggtagccgc cgcttgcgag tccggccagg
    14941 ctgtaggtgt tgtcctgaag cgtcgagccc gtactggtgt agtacgagct cgtcgaggcg
    15001 accgtggcaa acccggcgtc gtaactcaga tcgaggcttg cgaggttggt atagcgggac
    15061 cagggttgat cgatctgcga gaactcgcga tagggtccac ccgccggcag agtgctcgcc
    15121 gggtcgaggg gtgaagggcc accgcggaag tcgagattga cctgcggacc gccatcacca
    15181 tgggagctcg aatcgaggat ggcggcttcg gcgctgaagg cgtccgtcgg cttccacaga
    15241 acactggcgc gtccggtgaa ggtcttctgg aaattccaat ccttctgatc ccgatagatc
    15301 gcggggctgg tcaccggatc ggcggaattg gccagcagcg gaatgcccga gagcccgttg
    15361 ctcgtgcgtg cgaagagccc gtaggtgtcg acgaagcccg gctcgtagta gtacttggcc
    15421 gccgcgcgga atgcgagcgt gtctccgagg gggacattga tgagaccccg caccgtgtac
    15481 ggggtcccat ccgaatggtc gagccgtccg gcactgcctt caaccagtcc cgaccagtga
    15541 ttcagctccg gcgagttggg aatgagtcgc agcgctccgg cgagtgtgcc ggcgccgtag
    15601 agcgtgcctt gcggcccacg caggacctcc acccgttgca gatcatcgag ctggaaatag
    15661 ccttcgatcg gtgagttgcc gatgtaagtg ccgacagggg cctgctcgaa ggtccggaaa
    15721 ccgcgggtcg gctcgcccgt cgcattgatg ccacggatga tcggcaccgt cgccgcggag
    15781 aagcgtgccc catactcata ggtgctgagg ccgggcactt gggaagccag cgaggcgata
    15841 tcggtcacgc ccgtctcggc gagctgatcg gccgtgacca ccgagaggct gtaggggact
    15901 gcctcgagcg tctgcgtgcg acgcgtagcc gtgacggtca cttcggcaat ggcgctcggg
    15961 gtggcttcat ccgccggggc ggcctgctgg gcctgggcgg ccggccagac cccgtagaga
    16021 gcgatacaga ccgccacccg caccgggctc aaggccttca cgatcggttg ggcaatactg
    16081 cttgcgtctc gatgctcgtg ctttcgcacc tgtgaacctc ccccatcttc gccggttcga
    16141 gacccgaaat gtctgccggc tgtgcgcgtt cccattcact atgtcgcatc ccaaggaggc
    16201 cggccacgcc ctttctttgg accgtcgcgg gacttcttag gaacgccact ttggcgctct
    16261 tgcgcaaaca gccgggcctg cgagaccggc ggccccgaac ggcgcgggcg gatgccggtg
    16321 ttcggggggc ctggccgcct agaattcggc ctctgagctc gcccgcgcca ggctctgcag
    16381 ggacgcatcg ctcgcgggct ggagcgccac tccgtgcggc ggctcggtct gcagcaagta
    16441 gcggacgaag taatcccagc gccggcgagt ggcgtaggcc gcggcaaccc cgacgggctc
    16501 gtgacgttgg ttcggcagca ggatcaggtc gaagtccttg ttcgccttga tgagcgcatc
    16561 caccagctgc agcgtctggt acggggggac gttgtcatcc atcgtgccgt gcatcagcaa
    16621 caagtggccc gcgagctttc ccgcgctcag cgtattcacc gagccgtcgt agctggtggt
    16681 gccgtccgca tgccgtacca gcaggcccat gaactcctct ccccagtcac cctcataccc
    16741 gaggacatcg tggaccccgc tcatcgcaat gccgaccttg aaaaactcag ggtagcgaaa
    16801 caaggcagcc gcgctcgccc agccgccgcc ggagatccca tagatgcccg cccgctgcag
    16861 gtcgatccag gagtcgcgct gtgcgagctc gcgcatgccg gcgacctgat ccgagagcgt
    16921 gttctcgcac agattgccgt aattcgtgtc ctggaaggcc ttggaccgca tggacgtgcc
    16981 catgccatcg atctgcacca cggcgaaacc gagctccgcg agcgcctgct catccccgag
    17041 cacgccggcc cgcaggaagg ttcgaggccc cacactgccg atctgcggcc ccgggtagat
    17101 gctgttgatg attgggtagc ggcgctgcgt atcgaagtgc gagggcaaaa acagcatgcc
    17161 gtacacgtcc gtccggccgt cacgaccctt gaccgtaaaa ggcagcggag gccgccagcc
    17221 cgcggccttg aggccctcga tgtccgcccg ctccaggacc ttgagcagcc gcccatgccg
    17281 atcgcgcagc acggtgacgg gcggtgtgtc cggggtcgaa tagcggtcca cgaagtactc
    17341 gagtgaggga tccatggaaa tcacgtgggt cgccttctcc ggagtgagca gctgcgcctc
    17401 gcccccatcg agcggcagcc ggtacaggtg tgcgaagtag ggatcgcggc ccacctcgcg
    17461 cccggccccg agcgcgtagg ctgcgcgccg ctttccgtca acgcccatga gcgcgatgac
    17521 attccacgct ccgcgcgaga tctgccgctt gagccgcccg gaggcggcgt catacaggta
    17581 aaggtggccc cagttgtcgc gctgcgagta ccagatgagt tctttgctgg cccacagata
    17641 gcgccagttc tcggcgtcct cggcccagcc ggtcgcgccg tggtagaagg tcggcacttc
    17701 ctcggtgagc acgtcacgaa cggtgccgct cacggtgtcc gcgacgcgga ctgtcacgcg
    17761 cttgtgatcg cgagacatcg acacgaaggc caactcgctg gcgtcggggc tccactgcag
    17821 gtcgggcatc cgtccgttct ggcagatctc atcccagcag tgcgtcgtgc ggcgctgctc
    17881 cggcggcatc tcgagacgca ccacggtccg cgaagccaca tcgatcacga cccgatgcag
    17941 catcggcacg gcctcatcac ctggcatcgc atatttccag gccgagagct gcggatgccc
    18001 gcgcatcgtt ctcaccaggt acatctcgcc cacctggcgc tggtcctgct ggtacgtcgc
    18061 gagacgacgc gagtcgggtg accagctcac gatggcgcgg tcgctatggt tccagcccga
    18121 attatccgtg gcataaccga agtccttcac cccatcgatg gtcaggggcg actcctgtcc
    18181 actcgccacc tcgcggaccc aaagattgtc gccgcggatg aaggcgatct gcttcccgtc
    18241 cggggacagc gcgcctgagc tatcgacggg ccccagcttg gtgcagcgtg gccgatcgag
    18301 ctcgcaggac cagcgatgcg cgaaagcctc gaaacggatc gctccggcgg acagttcgaa
    18361 gcgatggaac gggaggctcg tggcctcaac cgggacagcc gcgacagcgg agagcgcctg
    18421 cgccacctgg agatgatcga aggcgggcct cgagctgggg tgcgccggat ccacccacct
    18481 gaactcgctg cccgcggtgg tcgttcgccg gtaccagaag tcaccgccct gcacccagta
    18541 cgcggggccg gtcgcattca gcaccctggg cgccacgttg taagccatga agcgttccgc
    18601 ccgcgcatag tcctcagcag tcggtgccga ctcggccgcc cacaggcccg aagagaaggc
    18661 gcacaggact gtgagagcaa agcgagcaca gtgcggtgag ggcataggca tcggaggact
    18721 cttcacatag gtttcgcggt tcatcgggtt tcgcggttca ggggtacgca agcttagtgg
    18781 agcgaacgcg gcttgcgcag cgaacgtatt tagggctggc tctcgcattc ttcggcggcc
    18841 gcgcttcggt cgcggcctcc cggactgcgt ccgctggggg cgcggcaacg ccgccgggtt
    18901 tgctgtaagg ttgggcgcaa gagccagacg cgaacccatg catagctcca agccatttat
    18961 ggaggcgtac cggctgatgc aagcggggcg cccggcagaa gctgttgtgt ttgccgagcg
    19021 cgcggttgca ggcgagacca cgtgcgtctc tgcgcatggg ttgctcgcga cgcttttgct
    19081 tcaagtcgga agagcaaaag aggcggacga gatggtcacc cacgctctgc agtgctctcc
    19141 aggaagcgct gacgcatacg acgccctggc tcacgtttcc atgctgctgg ggaagcacga
    19201 gcgagccaac cttctctacc gtcgcgtcgt caagctcgcc cctgatgatg cgcggttctg
    19261 gtacaacctc gcctcgagcg agcgcagttt cggacgactc caggaagctg agtccgcctg
    19321 caaccgggcc atcgcactcg attcctctca ttacgcaagc tatcttcttc gctcggagtt
    19381 gcgcgtccag tctgccgaga acaatcacct tggggaattg caatcggcct tgtcccggcc
    19441 cggactcgac gagcgtgcac gcgtaccgct cggttatgcc ctggccaagg agctggacga
    19501 tctccagcgc tatagcgaag cctttcaata ctttcaggaa tccgcgcaga ttcgccgacg
    19561 acacctgtcg tattccatcg ccatggacga gcacaagatc gcgcgcatcc aacagatctt
    19621 cacgcccgat ctcatccaga ggcgggctcg cccggagggc tcaagccgct ttattttcat
    19681 cgtgggtctg ccgcgttcag gcacaacctt gctcgagcac attctgagcg gcttggagag
    19741 ggtcagatcg aatggcgaga cggactattt cgcgcgcgca ttgtttgcag ccgcccccgc
    19801 aggcccgcag gacgtgtttg cgcgcgcagc gctggcggat cccggtcggg tcgccgcgag
    19861 ctacgggcac tttgccgatc atgactccaa cggtacgaga atcatcgaga agctgccgct
    19921 gaattatctg tatcttggag cgatccaccg agcgctgccg caggccaagc tcgtgctggt
    19981 cagacgctcc cccatcgatg cgtgttttgc catgtaccgg acgctgttcg gtgtgggcta
    20041 tcccttcagc tacgactttg aagatctcgc acgctatcac gcggcatacg accagctgct
    20101 ggagcactgg cggaaaactc tcaaggaaga gctgtgcgaa gtggtctacg aggaactcgt
    20161 gagcgatccc ttgcaaacgg gggcgcgggt ggcacaacac tgtggactca cctggaaccc
    20221 gcgcgccatt gaggtcgaaa agagcgcgac gatctcgatg acggccagcg ctgcgcaagt
    20281 gcgtcgcccg atttacggct cttcatccgg gcgctggcga cattacatcc agcatctggg
    20341 gccgttgctc gaggcacttc gccgccaccg agtgatatct gcggacagcg tatagtttgc
    20401 acgctagtgg ccactcagag agcccattcc ctgccctcga agcgttgtcc cgaaagacgt
    20461 tgctgcttcg catcccgagc ttcgagggga agtaccggga tgctctggct cacttgatcg
    20521 gtgagaagcg cggcgaactc atggatcatg aagactggat cgtcgctgtg cggggaaatc
    20581 agggcggcag cgattcaacc tatgaggcct tgctaccctg gctcctggcg gatgaatatg
    20641 aagacgctgg ggcgaaatgg ctggtcactc ccgccaacat cgagggacag cgaaacctct
    20701 gcagactgat ggcgccgggt gacagggtct gcgagagcta ttcggcgcag gcgatacagc
    20761 gcatggaagg tgcggccgcc ggcacctacg tctcgcagaa ccgggtcccg agatccacta
    20821 ccggcgtatc gcccatccgg agtctgtcgg ccggccgaaa cgcgtcgccg tcctgttcga
    20881 caagggttgc ggcacgtgca ggggcgcctg tcgtgcagat tacggacggc agcctccgcg
    20941 gtagcaacct tggcgatgtt gagggtaagc cgtcctgatg gggaggagga acatcagccg
    21001 ccgagagccg ttgccaccgt aggccgagtg ggatgcccca ttacgatgag cacaacctgg
    21061 tggcagcgtg gagtgatcta ccagatctat ccgcgctcgt ttcaggactc caatagcgac
    21121 ggcgtcggcg acctgcacgg aattcgtgcc cggctgcgct actgcgcggg cctcggagtc
    21181 gatgctctgt ggctctcgcc catctatccg tcccccatga ccgattttgg ctacgacatc
    21241 tgcgactaca ccggcataga tcccgtcttt ggtactcaga cccagttcga tgagctcatc
    21301 gcagaggtca agcagctcgg gatgaagctg atcctggatt acgtgcccaa tcacacctcc
    21361 gatcagcacc cctggttcaa ggagtcccgc tcctcgcgga cgaacccaaa acgcgactgg
    21421 tatctatggc gggatcgggc tccgggcggc gggccgccca ataactggct gagccagttc
    21481 ggcggcagcg cctggcagtg ggacacggtc accgctcagt actactatca cgccttcctg
    21541 cagacacagc cggatctgaa ttggcgcaat cctcaagtac gcgccgccat gtacgatgtg
    21601 ttgcgcttct ggctccgacg cggcgttgat ggcttccgcg tcgatgtcct ctggcatctc
    21661 atcaaggacg atcagtggcg cgacaatccg cccaaccctc gatatctacc gggtgagccg
    21721 ccgcagcata gccagatccc gctctataac acggatcggc cggaggttca gcaagtggtc
    21781 gtcgagatgc gcaaggtcgt ggacgaattc cccgaccgcg tgctgatcgg cgagatctat
    21841 ctcccgctcg agcggctcgt cgcctattac ggcgcgcaac tcgacggtgt gaatctgccg
    21901 ttcaactttc atttgctgct gacggcctgg aacgcgcgcg ccatcgcgac ctggatcgaa
    21961 cgatacgagc gtgccctgcc ccctggagga tggccgaact gggttctcgg caatcacgac
    22021 aaccccagaa tcgcctcccg cgtcggcatt ctgcaggccc gtgtcgcggc gatgctgctc
    22081 ctcactttgc ggggaacgcc aacgatgtac tacggggatg agatcggcat gccgaacacg
    22141 ccgattcctc ccgatcgggt tcaggacccc tatgagaaga acgtcccggg catcggtgtt
    22201 ggacgcgatc cgtcacgcac gcccatgcag tggaccgcct ccgcgaatgc cgggttcagc
    22261 gaccacgaac cctggctgcc gatcgccgcc gactatgagg tggtgaacgt gcatacggag
    22321 gacgccgacc ccagatcgat cctggtgctg tatcgcaggc tcctggcgct gcgccgatct
    22381 catccggccc tcatcgaggg cgcctatgag cccgtgacca tgaccggtga tctactcgcc
    22441 tacgtgcgtc attccccccg gggcaggctc ctgatcgcac tgaatctggg cgcgggcccg
    22501 ttcgatttgc tgttgggctc gcaaggcatg cgcggccgta tcctgctgtc cacgcatctc
    22561 gatcgggaga ccggcccgga gttggcagac ctcgcactgc gtgcaaatga gggcgttatc
    22621 ctggagctcg ctcgtgatca atgacctctg gtacaagaat gcggtgttct attgcctgtc
    22681 cgtcgggacg tacatggatg ccaacgggga cggcatcggt gatttcgaag gcgtcgcgcg
    22741 gcgcctggat tatctgcaag ggctcggaat cacggcactc tggctcatgc cgtttcagac
    22801 ctctcctggg cgcgatgatg gctatgatgt cagtaactac tacgaggttg acccgagata
    22861 cgggacgctg ggagatttcg tagagttcac gcacgcggct gcgcaacgag gcatccgcgt
    22921 catcatcgat ctggtggtga accatacctc ggaccagcac ccctggtttc aggaggcgcg
    22981 ccgggatccg cactcgaagt accgggactg gtacgtctgg tcacggaagc ggccaccgga
    23041 ttcgagcaag ggcgtggtct ttccgggcgt gcagaagtcc acctggtcgt atgactccgt
    23101 ggcccgcgcc tggtatttcc atcgtttcta cgactttcaa ccggatctga acacggccaa
    23161 tccacaggtg ttgtccgaga tcctgaagat catgggcttc tggatccagg ccggcgtgtc
    23221 cggattccgc atggatgccg tgccgttcgt cattgccacc aagggcccgg cggtccgtcg
    23281 ccccctcgag caatacgaga tgctgcgcgc catccgcgag tttctgcagt ggcgcaaggg
    23341 cgatgccatc gtgctcgccg aggccaacgt gctgcccgat accgatatgg agtacttcgg
    23401 acgcgacggc gatcggatgc acatgatgtt caattttcag gtcaatcaga atctgttcta
    23461 cgcgctggcc gcccaggatg tgcgtccgct cagcgtcgcg cttcgcaaga ccaaaccccg
    23521 tcctgacacc gcgcagtggg gccaattcct gcgcaaccac gatgagctcg atctcggccg
    23581 gctgaccgac ccgcagcgcg aaagggtatt ccgcgaattc ggtcccgagc ccagcatgca
    23641 gctttaccgg cgaggcatcc gccggcgcct ggctccgatg ctggccggcg accggcggcg
    23701 catcgagctc gcctatagcc tcatgttcac gctgcctggc accccggtca tccggtatgg
    23761 ggacgagttg ggcatgggcg atgatctaaa gttgcccgag cgcaactgtg cgcgtactcc
    23821 gatgcaatgg tcgacggagc cgaatgccgg attcaccaaa agtgacaagc ccatctcacc
    23881 cgtgatcagc tcgggaccct acggctatga gcacgtgaac gcggcgcagc agcgtcgcaa
    23941 tcccgaatcg ctgctcaact ggaccgagcg catcatccgg atgcgcaagg aagtccccga
    24001 gatcggctgg ggtgacttcc agctgctgcc cacgggcgat gtgacagtgc ttgcgatccg
    24061 gtatgactgg cgcaataact ccgtcgtttt cgtgcacaat ctttccggca aaccgcgtga
    24121 gctgactttt gacgtcggcc tgcagagcga ggacgcttca ctgctgatca atcttctctc
    24181 ggatgatcac agtcgcgcgc gccatgggcg ccatcacata gtgctggaag ggtatgggta
    24241 tcgatggttt cgcgtgggtg ggctggatta cctcctgaag cgcagcaacg tagaggtgca
    24301 gtccaaagtt cacaaaatag cgcgacgacg caagcggtag ggtgctgcat tgcggggagc
    24361 atcttccatg agcaatgggc atgaaacctt cgcaggagtc attcggcttt cttccagcca
    24421 cactgtcccg gagacgctgg acagactcga gaggctgctc aaggagcacg gggtgacgat
    24481 cttcgcgcga atcgacttca gcggcgatgc cgcgcgagcc ggctctccat gccacccgag
    24541 cagcagttga tctttggcaa ccccaaagcg ggcacgccct tgatggtgag ccagccgacc
    24601 gccgcgctgg atcttccgat tcgggcgatc tgctggcagg actcactcgg aaaaacctgg
    24661 attgcctaca atgcgcccga atacgtggtg cagcgtcatt ggctacccgc ggagttcgcg
    24721 aagaatctgg ccgcagtgat tcctctgctc gaacgcgcag cgggtcgcag ctaggtgccg
    24781 gcggttccgt aatttcgggc gagatattcg gcaaccgaat cgatctggtc atcgggaatc
    24841 ggcgccccgt agaccttctt catcttctcg acctccgctt tccagaacgc cagtggctta
    24901 tcgggaggct gcgtgatgat gtagtcggcg gaatggcaga tgaggcactg ggcctgggtc
    24961 agatctgcac ccgggcctgg cttgagggtt gccgtctcct tcggcaattc gatcctgaga
    25021 ctggcagcga ggctgcccgt tgcgaccaca gctccgagca gcgcgatacc tatgacctgg
    25081 gcgtactgca tgatttcccc tacgcggcgg tgacgcgcgt tgtctccacg acgttgcgca
    25141 tgtagccaga ggggttccag agcgcttcca gcggctgaga ctggccgatc acgttgactg
    25201 cacggacctg gatcgcgtgc gctccgcgtt caagcgcgac cgtggtctgc cactgccgga
    25261 aggaatattt gccgagatcc ttgccgagcg tggtttgcct ccaggttcgg cccccgtcca
    25321 ccgacaggtc cacttcgcgt atgccatacc cgccgtcgaa ggcgatgccg cgtacgacga
    25381 gaggtcgccc tgccctcacc gtcgaaccct cctttaggtt ggtgacgaac gagcggatgg
    25441 cgaaccggct gatgggaacc gtctttttcg acggcgtccc cggcggcacg cacgcacaag
    25501 cgttgtcggg aattcggtag gcagtggtca tccagaagtt gtcgagaacg ctatcgagca
    25561 ccgttatctc attcagatgc ttgacccagt aggtgccgaa gtaaccgggc acgacgaggc
    25621 gcacgggata gccgttcagc atcggcaggt ccgcatcgtt catcgagtaa gcaatcatca
    25681 cctcgccatc catggcgtga tcgacctcga gtgccttggc gaagtccggc gtcgcgggga
    25741 gcacgggccc atccagacca tcgaacctca cctgtttggc gccggcctga acgcccgcct
    25801 tgtcgagaac cgtcttcagg ggcacgccgc
//
LOCUS       DASRHN010000274        22243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1416094, whole genome shotgun sequence.
ACCESSION   DASRHN010000274 DASRHN010000000
VERSION     DASRHN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22243
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416094"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFB37_11015"
     CDS             complement(<1..541)
                     /locus_tag="VFB37_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO23021.1"
                     /translation="MSYQPVRIPRHEYISVRGLQHRLTWWGEPGQRPVVLLHGFMDCG
                     DTWQFLVDCLPPQWSLVALDWRGFGGSDWAPGGYWFADYLADLEVLLDALTPGTPARV
                     IGHSMGANIAALYCGVRPQRLAWLVSLEGVGLPDTRADMAPARYAQWLDELRESPRGN
                     RYARIEQLAGVLQQRNARLT"
     gene            608..1003
                     /locus_tag="VFB37_11020"
     CDS             608..1003
                     /locus_tag="VFB37_11020"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HZO23022.1"
                     /translation="MNDSKPSAGAAAAEPLAVPSQEERGWGMLAHLSAFAGLIMPVAG
                     NVLGPLLIWYLRREQSAFVSEQAKEALNFNICVLIGFAVCGVLTLLLVGFLLGMGLLV
                     FWIAATIIAGLKAGEGVHYRYPFSVRFVK"
     gene            complement(1007..2524)
                     /gene="ilvA"
                     /locus_tag="VFB37_11025"
     CDS             complement(1007..2524)
                     /gene="ilvA"
                     /locus_tag="VFB37_11025"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HZO23023.1"
                     /translation="MASPYIERILKARVYDVAIESPLEEAPRLSSRLENQVRLKREDL
                     QPGFSFKLRGAYNRIAHLSAAIAQRGVVCASAGNHAQGVALAARRRGIPAVIVMPQTA
                     AQIKAQAVRDLGAEVLLQGDDYDAAFDRALVLARERSLVFVHPFDDPDVIAGQGTIAV
                     EILRQSGGQLDAIFVPVGGGGLIAGIAVYVKYLYPQVRVIGVEPEDAAGMYESLKAGR
                     RTPLERVGIFADGVAVKRVGEETFGLCRAHVDEILLLSTDEICAAIQDVFEDTRSIVE
                     PAGALAVAGIKKYVAREGCRGKRLVAINSGANMNFDRLRHVAERADLGAQREALLAVL
                     IPEEPGSFLRFCELLGRRSVTEFNYRLEPGHAAQIFVGFGISAGCGERDGVVRSLREA
                     GYTVTDMTDNEMAKLHVRYMVGGHVRGIQNELLYRFEFPERPGALLRFLQAVGSRWNI
                     SLFHYRNHGSDYGRVLAGIQVPPAERPDFVLHLNELHYAYVEEGANPAYRLFLGA"
     gene            complement(2558..3382)
                     /locus_tag="VFB37_11030"
     CDS             complement(2558..3382)
                     /locus_tag="VFB37_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZO23024.1"
                     /translation="MLRLPGGHAFCSLSTTRTPELDSKQATRLAAVGDLHVKKTSQGT
                     LQRALASVNERADLLLLCGDLTDYGLPEEAEILARELTAAVSIPVTAVLGNHDYECGQ
                     QAEVSRILTQAGVKVLDGEAYEVQGTGIAGAKGFSGGFGRATLGAWGEQSTKRFVQEA
                     IDEALRFEGALARLRTERRVALLHYSPIRATCEGEPLEIFAFLGTSRLEEPLNRHPVD
                     MVFHGHAHHGGLEGRTANGTPVYNVAMPLLLRTFPDSPPFRVVEIAQPSPAGAENR"
     gene            3401..4198
                     /locus_tag="VFB37_11035"
     CDS             3401..4198
                     /locus_tag="VFB37_11035"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HZO23025.1"
                     /translation="MAVSCGPAPGARQFYVHTMSILNEAGLDYLVGGGHALEHYTGIE
                     RQTKDFDIFVRRDDYERIMRVLDDAGYHTDLTFPHWLGKASCEHGYIDVIFNSGNGVT
                     VVDDAWFQHAAAAQVFGVPARICPAEEIIWSKAFVMERERYDCADIMHLLLTCATRLD
                     WPRLVGRFGAHWRVLFSHLSVFGFVYPSERARIPDWVMSRFLGLLEMELREPPPTHRI
                     CQGTLLSREQYLVDVQRWGFADVRQTGASTMTASEIVLWTEAIQDSK"
     gene            complement(4277..6328)
                     /locus_tag="VFB37_11040"
     CDS             complement(4277..6328)
                     /locus_tag="VFB37_11040"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZO23026.1"
                     /translation="MSLQRHSSPPDEADLETTAELPVLDVMAYEAGLTPGASSSAGPG
                     SVARSAASGPAREHLGSTDTWIVPAPGLRVAPPPASEVASAAIDENRARLEINLQALS
                     TTLRDVEERLTRKGERLAEIERALETANAERAASEQRARSLSNELAQVRSGASAAQTR
                     VAELHKTLQDRQAADEASRAREAEAHTLLAQRTSEFRAQLNASQHDFEIKLEARAQAF
                     TVIQQELEATRSRATGYLETLQSNEGRRAVFEQLLSSLDTDIVQRDTRLSRLESDLAA
                     HLTRVAQLESELRERVARIATLDKQVSALSAALAQRGEQQTDTERARDNLQQTVHALN
                     AALSQRNDRIRSLEESSGQQNATAAQQAAELERLSGERAQLLATAASLESRLKSANAR
                     GDEQESVARAAQARCGELEEALTAQRHRGEQLESEVATVRSELLTSSTALHSAGSERN
                     EQIAQIAATEARIGELEARFAEQQETVRLLQAEANASVARVKELESDLRAAEDTIHRL
                     ETEARSKAARVEELEKINQEWRATVEEARQAITERDNLIHRLEQETANSAVLVGHIQR
                     SITRLDHGTEHTHEPAPDGATRLLIRTDGDSEVVHVLGRKTSIGRTPDNDLQIDAKFI
                     SRHHAVILAGPAHAIIEDLNSTNGVLVNNRRITRQPLKDGDNVVIGKTQFRFAVRAAK
                     G"
     gene            complement(6495..7334)
                     /locus_tag="VFB37_11045"
     CDS             complement(6495..7334)
                     /locus_tag="VFB37_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO23027.1"
                     /translation="MAKANVPHRQVETEPRVRRGYFECRYGQFHVHNAIPPGGGFEEG
                     TALLCVHSAMASGRFFQGLLPLAGRDRSVYAPDLPGFGESDSPPARVGIPDYAAALGD
                     FLDTMRFRQIDLLGHQTGSLIAAELALARPQQVRRLVLASVPVLNDAERDGIRRHPGL
                     AATEDGAHLATEWRRTLESYGPAVPLEVRARAFAEKLRSGMQGAWMLAAAQQYESRQR
                     LSRIVQPALVLRLKDEYWDATPRVRELLPKARFAEVPASNAAVLETEPETILELVRDF
                     LKG"
     gene            7519..7731
                     /locus_tag="VFB37_11050"
     CDS             7519..7731
                     /locus_tag="VFB37_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23028.1"
                     /translation="MSRLIPLMLLILPLAGCGLAETGAAAGAGAASEVEQARQAKQTE
                     ARVQQQVDAAYQQAAAQRKAAEAASQ"
     gene            complement(7795..9045)
                     /locus_tag="VFB37_11055"
     CDS             complement(7795..9045)
                     /locus_tag="VFB37_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO23029.1"
                     /translation="MSNADIRGRFIWHELMTTDPEAAGAFYTNVIPWKTQPSGMPSYT
                     LWMSGKNQAGGLMALPHAEAGTPPQWIIYVGTPDVDSSAEAARRLGGKVLKPPADIPN
                     VGRFAVLSDPQGAAFAIFTPKPGSGDGASTAGAVGDFTWHELATTDLDGALTFYTDLF
                     GWSKGAAHDMGPMGVYQLISHRGQDVGGIYKARDASTPPSWLSYTRVADAAKAASAVK
                     ANGGRVLNGPMEVPGGSWIVMALDPQGGAFAVVEAPKAAKAEAPSAQKKPAEAAKPAQ
                     PSPAGKPPSAPAAVPPAKAAPAASAGKAEAASQSRTSAPPAAAATSSAAGSRTRAPAK
                     RPSAKKAGAKKTAAARKTGGRKTAAKKAGSRKTGAKSSSRRSKTRKRSAVKRAASVRG
                     RKGRGKSARRAPGRSTRRASRKRK"
     gene            9238..10362
                     /locus_tag="VFB37_11060"
     CDS             9238..10362
                     /locus_tag="VFB37_11060"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO23030.1"
                     /translation="MNRRGFIHVVAAVAAAATSARRAFAADHGGRAARPLRILILGGT
                     RFIGVHMTQLALDRGHTVTLFNRGKTNADLFPQVEHLHGDRDGQIDALRGHKWDAVID
                     DSGYVPRHVRLTAELLSPNVSQYLFISTISVYASLAKPVTEESPVGKIPDESVEKVDN
                     TTYGPLKALSEKAALAAMPGRTTIFRPGLIVGPDDSTDRFTYWPARAARGGDFLAPDS
                     PHDPIQFIDARDLAAFALRSVENRVVGTFNVVSPPGKFTIGELVDASIAAANAQVKPE
                     PPPHAVWVPVDFLERQKVEPWMDMPAWVPEKGDEAGAMHTSAARAMHAGLQIRPIAET
                     VSDTLAWHLKRPKAEQDSLKAGIAPQREQEVLSAWKQLKG"
     gene            complement(10478..10936)
                     /locus_tag="VFB37_11065"
     CDS             complement(10478..10936)
                     /locus_tag="VFB37_11065"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HZO23031.1"
                     /translation="MALEETPRRMERYLRENIPLSAAMGVRVKAAAPERVILCAPLAP
                     NINHHDTVFGGSAAALATLSAWTLLHLRLEQAEAAVQLVIQRSSMEYERPIPGDFDAE
                     CAFADAAAWERFLATLDRRGRARLSLTAQLLHATQRMASFTGDFVALRAG"
     gene            complement(11030..11947)
                     /locus_tag="VFB37_11070"
     CDS             complement(11030..11947)
                     /locus_tag="VFB37_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZO23032.1"
                     /translation="MQEPSAGRPLKAIATMILAVSAFCGLDTLLKIFSQHYPPMEVAT
                     IRGAASLPFMLVPIFITRRYSDLRPKRLGMHLLRAVLMLITLGGFIYAVRALSLANAY
                     AIFLAAPLIVTALSVPILGEYVGWRRWVAISVGLAGVITMLHPSASSFISVGALAALL
                     SATTYAFSAILLRVLTRTDTTASVVFWMIGIMTVFAGMIAAPDWVAVRHEDWRMLIGI
                     GVFAATGQHLLTEAFRQAPPAVVAPFEYTALLWGILIDWAVWGVFPGTRLYLGGGIVI
                     GSGLYLIWREHSGSVPPVASETAARTTLP"
     gene            complement(11953..14613)
                     /locus_tag="VFB37_11075"
     CDS             complement(11953..14613)
                     /locus_tag="VFB37_11075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO23033.1"
                     /translation="MKLVPGLLIAAAFAALTCAVWAFLNRPASEPPWPARIQGFAFSP
                     FRLSEDPTRHELPTDAEIDSDLKLLAGKVNAVRTYSVDGSLGDIPQLAERHGLNVAVG
                     AWIDDQRDHNERELDKAIELARTHLNVMRVFVGNEVVLRGDVPVKELEADLDRVREAI
                     GQPVGTAEPWHVWIEHPELAQHVDFIGIHLLPYWEGVGVETAVGYSLEQFHRVQKAFP
                     GKPVIIAEVGWPSHGRTRVFSVASEANEALFLRRFLKQAEHEQIIYYLMEAFDQPWKA
                     YMEGSPGSYWGVYDVNRRPKFAFTAAIVRMPEWHMLAAISVAVALLVLGVLYFNSATL
                     RNRGRSFLAVIVYAAATITVWVFYDFTQQYMTVSSVVSGVLLLLGMLGVILVLLAEAH
                     EWAEAHWVTSRRRLAHPCRDSGEPQPKVSIHVPAYNEPPAMLAETLDALARLDYGNYE
                     VLVIDNNTADEAVWRPVEALCARLGERFRFFHVAPLEGFKAGALNFALHQTAADARII
                     AVIDSDYVVSPSWLREMVPGFRDPRTAVVQAPQDYRDERQSCFKAMCFAEYRGFFHIG
                     MITRNERNAIIQHGTMTLVRRSVLQSTGGWAQWCITEDAELGLRIFEAGYEASYVPSS
                     YGRGLMPDTFVDFKRQRFRWAYGAMQILRSHAGSLFTEGGPLSAGQRYHFIAGWLPWV
                     ADGCNLLFNVAALGWSAAMILAPRQIDPPLVMYSVLPLSLFTFKLAKLVHLYRVRVGA
                     NLRQTIAAAVAGLALTHTIGTAVIKGLLTRGEPFFRTPKGGHAPGLRQALAAARNETL
                     MMLALWFCAWGVSHATTLQGLDRFSSTTMQGPDRFAWIVVLMIQSVPYASSLVASLVS
                     AFALPAHWLGEGYRASITSSPSRPEADTVS"
     gene            complement(14769..15188)
                     /locus_tag="VFB37_11080"
     CDS             complement(14769..15188)
                     /locus_tag="VFB37_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO23034.1"
                     /translation="MKYLHTMVRVTDVEASLRFYCQALGLKELSRKEVPQGRYTLIFL
                     AAPDDENAQVELTHNWDPQPYPAGRNFGHLAYAVDDIYATCERLREHGVTINRPPRDG
                     RMAFVRSPDDISIELLQKGAALAPAEPWKSLPNVGVW"
     gene            complement(15191..17038)
                     /gene="ilvB"
                     /locus_tag="VFB37_11085"
     CDS             complement(15191..17038)
                     /gene="ilvB"
                     /locus_tag="VFB37_11085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZO23035.1"
                     /translation="MSDTVIQPRPVAHPLAGQRMSGARMIMQVLADEGVTAIFGYSGG
                     AILPTYDAVFLYNEQRVREGGAQIPLIVPANEQGAAFMAAGYARSTGRVGVCMVTSGP
                     GATNTVTPVRDAMADSVPLVVICGQVARASIGTDAFQEAPVPSIMGAIAKHIFLVTEP
                     ERLEATLRTAFEIARTGRPGPVVVDVPKDVQNWEGPFLGGGMLPIPGYRRRMQELTAR
                     ALGEEQTATFFEMLAQSRRPLIYAGGGVINANAASALAHFADTFGIPVVTTLMGIGAV
                     DTTLPLSMRMLGMHGAAFANYAVDDCDFLIAVGARFDDRVAGVPAKFAPKAQRIAHLD
                     IDRAEVNKVKRAHWSHVGLIAEALERLTAQGRRQGLHPDYRQWHAELAELKRVHAMNY
                     DRASALIQPYYVIEEINRHAAGNAIISTGVGQHQMWAAQYCDFRQPRLWLTSGSMGTM
                     GFGLPAAIGAQIANPGRLVIDIDGDSSIRMNIGELETVTTYELPIKIVVLNNFGDGMV
                     KQWQKLFHKGRLSASDRSLHKKDFVKAAQADGFHYAVRLDRKEDVPRVIDELIHFDGP
                     AFLEVMIDPDAGVYPMVGPGQSYDEMITGDFIPSRRKVQIKTPGASEMF"
     gene            17194..17694
                     /gene="ilvN"
                     /locus_tag="VFB37_11090"
     CDS             17194..17694
                     /gene="ilvN"
                     /locus_tag="VFB37_11090"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZO23036.1"
                     /translation="MRHIIAILLQNEAGALTRVAGLFSSRGYNIESLSVAATDDPTVS
                     RLTLVTKGSEGVIQQIANQLNKLVDVVSIEDMTQGEHLERELILLKLRVQPEQTDAVR
                     GYIVRTGGRVLDPATDGFIAELTASEAEINDFVAHLGRQAALLEVVRSGALGIGRNAR
                     PLRVVR"
     gene            17713..19302
                     /locus_tag="VFB37_11095"
     CDS             17713..19302
                     /locus_tag="VFB37_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO23037.1"
                     /translation="MTRARILIVDDLPEKLLVYSSLLDDIDAEIVTARSGTEALKRVL
                     DGQFAVILLDVNMPDIDGLETATLIRSHRNGRHTPIIFITSYADEMQTTRAYELGAVD
                     YILSPVVASVLRTKVRVFVDLYEAHEALARSNRDLESRVKERTAELEQLSRLKDEFLA
                     TMSHELRTPLNAIFGWVTLLRTRRLDAPTQERALETIERNARAQKRLIEDLLDVSRIV
                     TGKVALELATFDPRRAIRGALDTMQPAAQAKDIRIISRLDSEVGTVRGDFARLQQIVC
                     NLLSNSIKFTQPGGEIEVSLTPFNESAQICVTDSGQGIKPEFLPLVFDRFRQEDGSIS
                     RRHGGLGLGLAIVRHLVELHGGAVEAHSDGEGKGSRFIIRIPTRAQIGDLAGAEDTSP
                     APASSSMLAGLRLLVVDDEPGARELISNVLRGYGAEVSVADSGQLALTKLFEVRPHVL
                     IADLGMPGMDGYALIEQVRALEPEFGGQTPAIAVTGYASPSDRLRALQAGYQNHVAKP
                     VEPEELAIVISSLSERKPRLS"
     gene            complement(19390..20379)
                     /locus_tag="VFB37_11100"
     CDS             complement(19390..20379)
                     /locus_tag="VFB37_11100"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZO23038.1"
                     /translation="MSASTRARVIRFHEVGGPEVLRLDEMDVGPPGPEEAQLRQTAVG
                     LNFIDVYDRTGLYPTSLPSGIGREAAGVVMALGRKVRGFKVGDRVAYVLPRPGAYCDV
                     RNVPADRLVKIPKGVSDQQAAAIMLKGLTAHYLLRRTYRVRRGEDILVHAAAGGVGLL
                     LCQWAKALGAKVIGVVGSEAKADLARKNGCRHVILLGKEDLVARVKQITRGAGVAVVY
                     DSVGKDTFFQSLDSLRPLGMMVSFGNASGPVPPIAPLELSKRGSLFLTRPTLFNYTST
                     RAELQAAAKELFAAVKSRAVRVRVGQTYPLAEAARAQTDLEARRTTGSTVLLP"
     gene            complement(20387..20725)
                     /gene="glnK"
                     /locus_tag="VFB37_11105"
     CDS             complement(20387..20725)
                     /gene="glnK"
                     /locus_tag="VFB37_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZO23039.1"
                     /translation="MKLVTAIIKPFKLDDVRAALSDIGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTRIEVAVRRDLVDQVIEAILKSAKTGKVGDGKIFITDIERVIRI
                     RTGETDDAAL"
     gene            20890..22125
                     /locus_tag="VFB37_11110"
     CDS             20890..22125
                     /locus_tag="VFB37_11110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO23040.1"
                     /translation="MLSRVFGQIVIVGAGQAADQAIHTLRRKGFAGKLLLVGDEPWLP
                     YQRPPLSKKFLAGTLERERLGLHPAQFYVEHQVETRLGQRVQQIDRGARRIRLDDESV
                     VPYDALLLATGSKPRALSVPGAHLPGVHSLRTLGDVDRIRADMAAGGRLVIVGGGYIG
                     LEVAATARELGMQVTVIEMADRVMNRVTCKEISSFYESEHSRHGVRIISNARVQALAG
                     DSRTGRVRAVLTEDGVEHPADLVIAGVGVVAADELALSAGLECSNGIVVNEYCATSDP
                     AIFAAGDCTNHPNPHYGRRLRLESVDNAYEQAASAALNLLGVPTPHDKVPWFWSDQFD
                     LKLIIVGICQGHDTVILRGLPAGRSFSACYLRQGELIAVDSVNSAKDQMAARKLIAAR
                     AHPDPGKLADPGILMRDCA"
BASE COUNT         3873 a         7335 c         7142 g         3893 t
ORIGIN      
        1 gtgtcagtcg cgcgttacgc tgctgtaaca cgcccgcgag ctgctcgatg cgagcgtagc
       61 gattgccacg cggggactcc ctgagctcat ccagccattg cgcataccgc gccggggcca
      121 tgtcagctcg tgtatcaggc aacccgacac cctcgagact gacaagccat gcaagccgct
      181 gcggccgcac gccgcaatac aaggcagcga tgttcgcacc catgctgtgc ccgatgaccc
      241 gggctggggt gccgggggtc agcgcatcga gcaacacctc aagatcggcc agataatccg
      301 caaaccaata gccgccgggc gcccagtcgc tgccgccgaa gccgcgccag tcgagcgcca
      361 ccagcgacca ctgcgggggc agacagtcca ccagaaattg ccaggtgtca ccgcaatcca
      421 tgaacccatg cagcagcact accggcctct gcccgggctc cccccaccag gtcagcctgt
      481 gttggaggcc gcgcaccgag atgtactcgt gtcgcgggat cctgaccggt tgatagctca
      541 ttggaccgcc cggccggcgc cccccataat gccgttgcaa tgctgcccgg ctcctggagg
      601 caggcagatg aatgactcga agccatccgc gggcgcagcg gccgctgagc cgcttgctgt
      661 gccgagccag gaagagcgtg gctggggaat gctggcacac ctgtcggcat tcgcgggcct
      721 gatcatgccc gtagccggca atgtgctcgg gccgctgctc atctggtacc tgcggcgaga
      781 gcagtcggcg ttcgtgagcg agcaggcgaa ggaagcgctg aatttcaaca tctgcgtgct
      841 gatcggtttt gccgtatgcg gcgtgctcac gctgctgctg gtgggctttc tgctggggat
      901 gggcctgctg gtattctgga tcgcggcgac catcatcgcc ggtctcaagg ccggcgaggg
      961 cgtgcattac cgctaccctt tctcggtgcg cttcgtcaaa tagccatcag gcgcccagga
     1021 agagccggta agcgggattg gcgccctcct cgacgtacgc atagtgcagc tcgttcaggt
     1081 ggagcacgaa gtcaggtcgc tcggcaggcg gcacctggat gccggcgagc acccgcccgt
     1141 agtcggatcc atgattgcgg taatgaaaca ggctgatgtt ccagcgcgag ccgaccgcct
     1201 gcaggaatcg cagcaacgcc ccgggccgct ccggaaactc gaaccggtac agcaactcgt
     1261 tctggatacc gcgtacgtgc ccgcccacca tgtagcggac gtgcagcttg gccatctcgt
     1321 tgtcggtcat gtcggtgacc gtatagccgg cctcgcgcag gctgcggacc accccgtccc
     1381 gctcgccgca gcccgcggag atgccgaagc cgacgaagat ctgcgccgcg tggcccggct
     1441 ccaggcgata gttgaattcg gtcacgctgc ggcgacccag cagctcgcag aatcgcagga
     1501 agctcccggg ctcctccgga atcaggaccg caagcagcgc ctcacgctgc gcacccagat
     1561 cggcgcgctc ggccacatga cgcaggcggt cgaaattcat gttggcgccg ctgttgatcg
     1621 ctacgaggcg cttgcctctg cagccctcac gcgcgacgta tttcttgatg cccgccacgg
     1681 ccagcgcgcc cgccggctca acgatcgagc gtgtgtcctc gaacacgtcc tggatcgcgg
     1741 cgcagatctc atccgtgctg aggagcagga tctcatcgac gtgggcgcgg caaagcccga
     1801 atgtttcctc gccgacgcgc ttcacggcca caccgtcggc aaatatcccg acccgctcca
     1861 ggggcgttcg ccggcccgcc ttcaatgact catacatgcc ggccgcatct tcgggctcga
     1921 cgccgatgac ccgcacctgg ggatacaggt acttcacgta cacggctatg cctgcgatca
     1981 atccaccgcc gcccaccggg acgaagatgg catcgagctg tccaccgctc tggcgcagga
     2041 tctcgacggc gatcgtgccc tggccggcaa tgacatccgg atcgtcgaag gggtgaacga
     2101 agacgaggct gcgctcgcgc gccagcacca gggcgcggtc gaaggccgca tcgtagtcat
     2161 cgccctggag cagcacctcc gcgccgaggt cgcgcacggc ctgcgctttg atctgggccg
     2221 cagtctgcgg catgacgatc accgccggaa tcccgcgccg ccgggcagcc agtgcgacgc
     2281 cctgggcatg atttccggcc gaggcgcaca cgaccccacg ctgggctatt gcagcggata
     2341 agtgcgcaat gcggttgtag gcgccgcgca gcttgaagga gaacccgggc tgcagatcct
     2401 cgcgtttgag ccgcacctgg ttttccagcc ggctcgacag gcgcggcgct tcctcgagcg
     2461 gggactcgat cgcgacgtcg tatacgcgag ctttgaggat tctctcgatg taggggctcg
     2521 ccacgtgcgg tcccgttcgc gcaaaggggc gagcagctta cctattttcc gcgcccgcag
     2581 gcgacggttg cgcgatctcc acgacccgaa agggtgggct gtccggaaac gttcgcaaca
     2641 gcagcggcat cgcaacgttg tacaccggag tgccgttcgc cgtgcgccct tccaggccac
     2701 catggtgtgc atggccgtgg aagaccatgt cgaccgggtg tcggttgagc ggctcctcga
     2761 ggcggctcgt gccgagaaag gcgaagatct ccaggggctc cccttcgcag gtcgcgcgaa
     2821 tcggcgaata gtgcagcagt gccacacggc gctcggtgcg cagccgggcg agggctccct
     2881 cgaagcgcag cgcctcgtca atcgcctcct ggacgaagcg cttggtggac tgctcacccc
     2941 atgccccgag ggtggcgcgc ccgaatccac ccgagaaccc cttggcgccc gcgatgcccg
     3001 tgccctgcac ctcgtaggct tcgccatcga gaaccttcac tccggcctga gtaagaatgc
     3061 gcgacacctc cgcctgttgc ccgcactcat agtcgtgatt gccgagcacc gcggtcaccg
     3121 gaatgctgac cgcagcagtc agctcgcggg ccaggatctc cgcctcctcc ggcaggccgt
     3181 aatcggtcag gtctccacat aacagcagca ggtccgcccg ttcgttcacg gatgccaggg
     3241 cgcgctggag cgtgccctgc gatgttttct tcacgtgcag gtcgcctact gccgccaggc
     3301 gcgttgcttg cttactgtcc agttcgggtg tcctcgtggt cgagagcgag caaaatgcat
     3361 gcccaccggg aagtctgagc attccgacga gtacggttct gtggcggtct catgcggtcc
     3421 tgccccgggc gcccgtcagt tctacgtgca cacgatgtcg atcctgaacg aggcgggtct
     3481 cgattatctg gtgggcggcg ggcacgctct ggagcactac acgggcatcg agcgccagac
     3541 caaggatttc gacatcttcg ttcgtcgcga cgattacgag cgcatcatgc gggtgctcga
     3601 tgacgcaggc tatcacaccg acctcacctt cccgcactgg ctcggcaagg cgagctgcga
     3661 gcatggctac atcgatgtca tcttcaactc cggcaacggc gtcacggtgg tggatgacgc
     3721 atggtttcaa catgctgctg cagcacaggt gttcggagta cccgcgcgga tctgcccggc
     3781 cgaagagatc atctggtcca aggcgttcgt gatggagcga gaacgctatg actgcgcgga
     3841 tatcatgcac ctgttgctca cgtgcgccac ccggctcgac tggccacggt tggtcggccg
     3901 ctttggcgca cactggcgtg tgttgttttc tcacctgagc gtgttcggct tcgtctatcc
     3961 cagcgagcgg gcacgcattc ccgactgggt catgagccgc ttcctgggcc tgctcgagat
     4021 ggagttgcgc gagccgccgc cgacgcatcg catctgtcag gggacgttgc tttcgcgcga
     4081 gcagtatctg gtggatgtgc agcgctgggg cttcgcggat gtccggcaga ccggtgcgag
     4141 cacgatgacc gccagcgaga tcgtcctgtg gacggaggcg attcaggact cgaaatgagc
     4201 gctgtggcgc gctgccgccc tggtgcacac ccggcagcgc acctgcgccg ccggtgcccc
     4261 gcgagaggaa atccgctcag cccttcgccg cccgcaccgc gaagcggaac tgggtcttgc
     4321 cgatcacgac gttgtcgcca tccttcagcg gttgccgcgt gatgcgacgg ttgttcacca
     4381 gtacgccatt ggtactgttg agatcttcga tgatggcatg ggcggggcca gccagaatga
     4441 ccgcatgatg gcggctgatg aacttggcat cgatctgcag atcgttgtcg ggcgtgcggc
     4501 cgatgctcgt cttgcgtccg agaacgtgaa cgacctcgct atcgccgtcc gttctgatca
     4561 gcagtcgcgt cgctccgtcg ggagccggct catgggtgtg ttccgtccca tgatccaggc
     4621 gcgtgatgct gcgctgaata tggcccacga gtacagcgct gttggccgtc tcctgctcga
     4681 ggcggtggat caggttgtcg cgctcggtaa tggcctggcg cgcctcctcg accgtggcgc
     4741 gccattcctg attgattttc tcgagctcct cgacccgcgc agccttgctg cgggcttcgg
     4801 tctccagccg atgaatagtg tcttcggccg cccgcagatc gctctccagc tccttcacgc
     4861 gtgcgacgct cgcattggct tcagcctgaa gcaggcgcac ggtctcctgt tgctccgcga
     4921 accgggcctc gagctcgcca atgcgagctt cggtggccgc gatctgggcg atctgctcgt
     4981 ttcgctcgga gcctgcgctg tgcaacgccg tgctcgaggt cagcagttcc gagcgcaccg
     5041 tcgccacttc gctttcgagc tgctcgccgc ggtgacgctg cgcagtcagc gcttcttcga
     5101 gctctccgca gcgagcctgc gccgcgcgcg cgacactttc ctgctcatcg ccgcgagcgt
     5161 tggccgactt cagccgggac tccagtgagg cggcggtcgc gagcaactgc gcgcgttcgc
     5221 cggacaggcg ctcgagctcg gcggcctgtt gtgcagccgt ggcgttctgc tgcccgctgc
     5281 tctcctccag tgagcggata cgatcattgc gctgcgaaag cgcagcattc aacgcatgaa
     5341 cggtctgctg caggttgtcg cgcgcgcgtt cagtgtccgt ctgctgctcc ccgcgctgcg
     5401 ccagggctgc ggaaagcgca ctgacctgtt tgtcgagcgt cgcgatgcgg gccacccgct
     5461 cgcgcagctc ggattcgagc tgtgccaccc tggtcagatg cgctgcgaga tcggactcca
     5521 gccgagacag tcgggtgtcg cgctggacga tgtcggtgtc cagtgaagac agcagctgct
     5581 cgaacacggc gcggcgaccc tcgttcgact ggagagtctc caggtatcca gtggctcgcg
     5641 agcgagtggc ctcgagctcc tgctggatga ctgtgaacgc ctgcgctcgc gcctcgagtt
     5701 tgatctcgaa gtcatgctgg cttgcattga gctgagcgcg aaactcggac gtacgttggg
     5761 ctaacagagt gtgcgcttcc gcttcacgtg cgcggctcgc ctcgtcggcc gcttgccggt
     5821 cctgcaatgt tttgtgcagc tcggccactc gcgtctgggc tgccgaagcg ccgctacgca
     5881 cctgagccaa ctcgttggag agcgagcgag cgcgctgctc gctggcggcg cgctcggcgt
     5941 tggcggtctc cagcgcccgc tcgatctccg ccaagcgctc acccttgcgc gtgaggcgct
     6001 cctctacatc gcggagcgtc gtcgagagcg cctgcaagtt gatctcgagc cgcgcgcgat
     6061 tctcatcgat cgcggcgctg gccacttcgg aagcgggggg aggggcgaca cgcagccctg
     6121 gcgcgggcac gatccaggtg tcggtcgatc cgaggtgctc gcgagcgggt ccactcgcgg
     6181 cggagcgggc aacgctgcct gggcctgcgg aggagctggc gccgggcgtg agtcccgcct
     6241 cgtaagccat tacatcgagc actggcagct cggccgttgt ttccaggtcc gcctcgtcgg
     6301 gtggtgacga atggcgttgc aggctcatgg caagtctcct cgcgctcacc gcggatccga
     6361 ttcgcgcggt cccctttgtt cctttggcag aaagtgtagc gccggcgcgg accgtggacc
     6421 gctattcaaa atcaaatgtg tgagagctgt cccgcagatg tgacataagg accggccagc
     6481 cgccggaacg ctcctcatcc cttcaggaag tcccgcacca attccaggat cgtctcaggc
     6541 tcggtctcca gcaccgcagc gttggaggcc ggaacctcgg cgaagcgcgc cttgggcagc
     6601 aactcgcgga cccgcggggt cgcatcccag tattcgtcct tgaggcgaag cacgagggca
     6661 ggctggacaa tgcgcgagag cctctgacgc gattcgtact gctgcgcggc cgcgagcatc
     6721 catgcgccct gcatgccact cctcaacttc tcggcaaatg ctcgggcacg cacttccagc
     6781 ggaacggcag gaccatagga ctcgagcgtg cgacgccatt cggttgccag gtgtgcgcca
     6841 tcttccgtgg cagcgagccc ggggtgtcgc ctgataccgt cgcgctcggc atcattcagc
     6901 acgggaacgg aggcgagaac gagacggcga acctgctgtg gccgcgccag ggcaagctcg
     6961 gccgctatga ggcttccagt ctggtggccc agcagatcga tctggcggaa gcgcatggta
     7021 tccaaaaagt ctcccagcgc cgccgcgtag tctggaattc caacgcgtgc gggaggggaa
     7081 tccgactcac cgaatcccgg caggtctggc gcatagaccg agcgatcgcg gccggcgagc
     7141 ggcagcaagc cctggaaaaa gcgacccgag gccatggcgg aatgcacgca aagcagcgcc
     7201 gtgccctcct cgaagccgcc acccggaggg atggcgttgt gcacatggaa ctgtccgtag
     7261 cggcactcga agtaccctcg gcgcacccgc ggctcggttt cgacctgacg gtgggggacg
     7321 ttggctttgg ccatcgaaga gctccctaca cagcgcgcgg cgcgccagat actgccactt
     7381 ccggccccag ccgtatgctc tcggtcacga aagcagcgtc cgcgccttgc cccaatcagc
     7441 ctgaacgagg aaagctagca ccttcacggc tcatccagtt ccagcatcct acgacatgcg
     7501 catatcggga ggttcgacat gagcagactc atcccgctga tgctcctcat actgccgctg
     7561 gccggatgcg ggctggccga gacgggtgcc gcagcaggtg caggcgcagc ctcagaggtc
     7621 gagcaagccc gccaggcaaa gcagaccgaa gctcgcgtcc agcaacaggt ggatgccgct
     7681 tatcagcagg cagccgcgca gcgcaaagcc gcggaagcgg cgtcgcagta gccacgggtt
     7741 cgcgttacca gaagcgaagc cgcgcgtcga cgcgagcgac gcgcggctgg ctctctactt
     7801 gcgcttgcgg ctggcgcgcc gcgtgctcct gccgggagca cggcgggccg acttgccccg
     7861 ccccttgcgg cctctgacgc tcgcggctcg cttgaccgcc gagcgcttgc gcgtcttgct
     7921 ccttcgcgag ctgctcttcg cgccggtctt tctcgatccg gctttcttcg cagcagtttt
     7981 cctgccgcca gtcttcctcg cagctgctgt cttcttcgcg ccagcctttt tcgcgctcgg
     8041 ccttttcgca ggcgctcgtg tccgggagcc tgcagcggag ctggtcgccg cagcggcagg
     8101 cggtgcactc gtcctggatt gagaagctgc ttccgccttg cccgctgagg cagcgggggc
     8161 agccttggcg ggtggcaccg ccgccggcgc cgaggggggc ttgcccgctg gggaaggctg
     8221 ggccggtttc gcggcttccg caggcttttt ctgcgccgag ggggcctcgg cctttgccgc
     8281 cttgggggct tcgactaccg cgaaagcccc accctgggga tccagtgcca tcacgatcca
     8341 ggagccgccg ggaacttcca tggggccatt cagcacccgg ccgccgttcg ccttgaccgc
     8401 ggaggccgcc ttcgccgcgt ctgcaactcg tgtgtagctg agccagctcg gcggggtcga
     8461 ggcatcccgc gccttgtaga tcccgccgac gtcctgtccc ctgtgcgaga tgagctggta
     8521 gacccccatg ggacccatgt cgtgagcggc gcccttcgac catccgaaaa ggtccgtgta
     8581 aaaggtcagc gcgccatcca ggtccgtggt ggcgagctca tgccatgtga aatctcccac
     8641 tgcgccggcg gtggaggcgc cgtcccccga tcccggcttg ggcgtgaaga tcgcgaatgc
     8701 cgccccttgg gggtcggaga ggaccgcaaa gcggcccaca ttgggaatat cggcgggtgg
     8761 cttgaggacc ttgcccccga gccgccgagc tgcctcggcc gacgaatcga cgtcgggcgt
     8821 acccacgtaa atgatccact gcggaggtgt gccggcctct gcatgcggca gcgccatcag
     8881 gccgcccgcc tggttcttgc cggacatcca caaagtgtag gaaggcattc ccgagggctg
     8941 cgtcttccac gggatgacgt tcgtatagaa agcgccggcc gcctctgggt cagtcgtcat
     9001 caactcgtgc cagatgaacc gtccccgaat atcagcgttg ctcatagcga gttttcccct
     9061 tgtttgcgag ctgcatcatc caccgacgat ccgcgcgaca ttctgcactg ctacgacgat
     9121 cacttccacg acagatgcgc tcacgcgtgt aatccgatcg ttcacccgac ggtggcgact
     9181 gagacatttc agctcgcgta gaggacaatg gtcacaggca gatctagaac ggacgatatg
     9241 aacagacggg ggttcatcca tgtagttgca gcggtcgccg ctgcggctac gagtgccaga
     9301 cgcgccttcg ctgcggatca tggcggccgc gccgcgcgac ccttgcggat cctcatcctt
     9361 gggggcacgc gattcatcgg cgtgcacatg acccaacttg cgctcgaccg gggacatacc
     9421 gtcacgcttt tcaaccgggg caagacgaat gcggatcttt tcccgcaggt cgagcacctg
     9481 cacggcgatc gggacggcca gatcgatgcg ctgcggggcc acaagtggga tgcagtgatc
     9541 gacgactcgg ggtacgtacc gcgccatgtg cgcctgactg cggagctcct gagcccgaac
     9601 gtcagccagt acctgttcat ttccaccatc tccgtgtacg cgagcctcgc gaaaccggtc
     9661 accgaggagt ccccggtcgg caagatccct gacgagagcg tggagaaggt ggacaacact
     9721 acctatggtc cactcaaggc tctgtccgaa aaggcggcgc tcgcagccat gccggggcgg
     9781 accacgattt ttcgtcccgg gctgatcgtc ggacctgatg acagcacgga cagattcacc
     9841 tactggccgg cacgggctgc gcggggcggc gacttcctgg cgcctgactc cccccatgac
     9901 ccgattcagt tcattgacgc acgcgacctc gcggcgtttg cgctgcggtc ggtcgagaac
     9961 cgggtcgtcg gaaccttcaa cgtcgtctcg ccgccgggta aattcacgat cggtgagctc
    10021 gtcgatgcca gcattgcagc ggcgaacgcc caggtgaaac cggagccgcc gccgcacgct
    10081 gtctgggtcc cggtcgactt cctcgagagg cagaaagtcg agccctggat ggatatgccg
    10141 gcctgggttc ccgaaaaggg tgatgaggcc ggcgccatgc acacgagcgc cgcgcgcgcg
    10201 atgcacgccg gccttcagat ccggccgatc gccgagaccg tgagcgacac gctcgcgtgg
    10261 cacttgaagc gtcccaaagc ggagcaggat tcgttgaagg ccggcatcgc gccgcagcgg
    10321 gagcaggaag tgttgagcgc gtggaagcag ctcaagggct gactacccga ctccagagcc
    10381 tcgtggcgac agaatcccag gtggcttgag ccgccgcggc cgcaggactg cgcttagcgc
    10441 gctcgtcggc atgccgctgc gaggccggtc gagggcatca ccctgcgcga agcgccacga
    10501 aatctcctgt aaaggaagcc attcgctgcg tcgcgtgcag caattgcgcg gtcaggctca
    10561 gccgagcgcg gccacgccgg tcgagggttg cgagaaaccg ctcccaggcg gcagcgtccg
    10621 caaacgcgca ctccgcatcg aagtcgccgg ggatcggccg ctcgtattcc atggagctgc
    10681 gctggatcac cagctggacg gccgcctcgg cctgctcgag tcgcaaatgc agcagggtcc
    10741 aggccgagag agtggccagc gccgcggcgc ttcccccaaa tacggtgtca tgatgattga
    10801 tgttaggtgc gagcggagca cacaggatga cccgctcggg cgcagccgcc ttcacgcgta
    10861 cgcccatcgc tgccgagagc ggaatgttct cgcgcaggta tcgctccatc cggcgtggtg
    10921 tttcctcgag tgccatgcgc gcatgctcaa gcggaagtgc tccttgcgca agcctcgtca
    10981 cctcattcgc aacgcgcacc tgctcatcgc acattcttgc ttcttccgct caaggtaagg
    11041 ttgtgcgggc cgccgtctcg gatgcgaccg gcggcacgct gccggaatgc tcccgccaga
    11101 tcagatagag cccgctgccg atgacgatac ccccgcccag gtaaaggcgg gtaccgggga
    11161 acactcccca caccgcccaa tcgatcagga tgccccacaa caatgcggtg tactcgaaag
    11221 gtgccaccac ggccgggggc gcctggcgaa acgcctcggt gagcaggtgc tgccccgtcg
    11281 ccgcaaacac gccgatgcct atcagcatcc gccagtcctc atggcgcacg gcgacccagt
    11341 ccggagcggc gatcattccc gcgaacacgg tcatgattcc gatcatccag aaaacgacgc
    11401 ttgccgtcgt gtcggtacgc gtcagcacgc gcagcaggat ggcactgaag gcgtaggtgg
    11461 tggccgacag caatgccgcc agggctccaa cggaaatgaa gctcgaggcc gaagggtgca
    11521 gcatcgtgat gaccccagcc agtccgaccg agatcgcgac ccaccgccgc caccccacgt
    11581 actcacctag aatcggcacc gagagcgcgg tcacgatcag cggcgccgcc agaaaaatcg
    11641 cgtaagcgtt agcgagtgaa agtgcgcgca cggcatagat gaagccgccg agggtgatga
    11701 gcatgagcac ggcccgcagc agatgcatgc ccagccgctt gggccgcaag tcggaatacc
    11761 gccgcgtgat gaagatcggt accagcatga aaggcagtga ggcagcacca cggatggtgg
    11821 cgacttccat cggcgggtag tgctgggaaa agatcttgag cagggtgtcc agcccgcaga
    11881 aggcgctcac ggcgaggatc atcgtcgcaa tggctttgag cggccgtccg gccgacggct
    11941 cttgcacggc tctcagctca ccgtgtcggc ttcgggtcgt gacggtgaag acgtgatcga
    12001 ggctcggtag ccttcgccca accagtgtgc gggcagcgca aaggcgctca cgagcgatgc
    12061 aacgagcgac gaggcgtacg gcacggactg gatcatcagc actacgatcc aggcgaaccg
    12121 gtcggggccc tgcatcgtgg tcgaggagaa ccgatcgagc ccctgcagcg tggtcgcgtg
    12181 tgaaaccccc caggcgcaga accagagggc cagcatcatg agagtctcgt tgcgcgccgc
    12241 ggcaagggcc tgtcgcagcc ctggcgcatg cccccctttg ggggtgcgga agaacggctc
    12301 gccgcgcgtc agcagcccct tgatcaccgc cgttccgatg gtatgggtga gagcgagccc
    12361 tgcgaccgca gccgctatcg tctgtctcag gtttgctccg acccggaccc ggtacaggtg
    12421 cacgagcttc gccagcttga aagtgaacag cgacagcggc aacaccgaat acatcaccag
    12481 cggcggatcg atctgccggg gcgcgaggat catcgcggcg ctccagccga gtgcggccac
    12541 gttgaacagc aggttgcagc cgtcagccac ccaaggcagc cagcctgcga tgaagtggta
    12601 gcgctgtcct gccgagagtg gaccgccctc tgtgaacagg gatccggcat ggctcctcag
    12661 aatctgcatc gcgccataag cccagcgaaa gcgctgtctc ttgaaatcaa cgaaagtgtc
    12721 gggcatcaag ccgcggccat aactgctcgg aacgtagctg gcctcgtatc ccgcttcgaa
    12781 gatacgcagg ccgagctccg cgtcctcggt gatgcaccat tgagcccagc cgcccgtgct
    12841 ttgcagaacg ctgcggcgca ccagggtcat ggtgccatgc tggatgatcg cgttgcgctc
    12901 gttacgcgtg atcatgccaa tatgaaagaa gccgcgatat tcggcaaagc acatcgcctt
    12961 gaagcagctc tgccgctcgt cccggtagtc ctgcggcgct tgcacgaccg cggtgcgcgg
    13021 atcgcggaag cccggcacca tctcgcgtag ccagctgggg gaaaccacgt agtcgctgtc
    13081 gatcacggcg atgatccggg cgtcggccgc agtctggtgc aaggcgaaat tgagcgcacc
    13141 cgccttgaag ccttcgagtg gcgcgacgtg aaagaaccgg aagcgctccc ccaggcgggc
    13201 gcagagggcc tcgaccgggc gccacacggc ctcgtccgcg gtattgttgt cgatgaccag
    13261 cacctcgtag tttccatagt cgaggcgcgc cagggcatcg agcgtttcgg cgagcatggc
    13321 cggcggttca ttgtaggccg gcacgtggat ggacaccttc ggctgtggct cgccgctgtc
    13381 tctgcaggga tgcgcaaggc gccgccgcga agtgacccag tgcgcctccg cccactcgtg
    13441 cgcctcagcg agcaggacca ggatgacacc taacattccg agcagcagca gcacgccgga
    13501 cacgacgctc gagaccgtca tgtactgctg cgtgaagtcg tagaacaccc aaaccgtgat
    13561 ggtcgcggct gcatagacga tgaccgccag gaagctgcgg ccgcgattgc gcagcgtggc
    13621 gctgttgaaa tagagcacgc cgagcaccag cagcgcgacg gcgacggaga tcgccgcgag
    13681 catgtgccac tccggcatgc gcacgatcgc cgccgtgaag gcgaacttcg gtcgccggtt
    13741 cacgtcgtag acgccccagt acgatccggg cgagccttcc atgtaggctt tccacggttg
    13801 gtcgaacgct tccatcaggt aatagatgat ctgctcgtgt tcggcctgct tgaggaagcg
    13861 cctcagaaag agtgcctcgt ttgcctcgga ggccaccgag aacacccgcg tacgtccgtg
    13921 gctgggccag ccgacctccg cgatgatcac gggcttcccg ggaaaggcct tctggacacg
    13981 atgaaactgc tccagggagt agcccaccgc cgtctccacc cctacccctt cccagtaggg
    14041 cagaaggtga atgccgatga agtccacgtg ctgcgccagc tcgggatgct cgatccagac
    14101 atgccacggc tcggcggtgc cgaccggctg gccgatggcc tcgcgaacgc gatcgagatc
    14161 ggcctcgagc tccttcaccg gcacgtcccc gcgaagcacg acctcgttcc cgacaaacac
    14221 ccgcatgacg ttcagatggg tgcgcgccag ctcgatggct ttatcgagct cgcgctcgtt
    14281 gtgatcgcgc tgatcgtcaa tccaggcacc cacggcaacg ttgagaccgt gccgctccgc
    14341 gagctgcggg atgtctccga gcgagccatc cacgctgtac gtgcgcacgg cattgacctt
    14401 gcccgccaga agcttcaggt ccgaatcgat ctcggcatcc gtcggcagct catgccgggt
    14461 cggatcctca ctcagccgga agggggagaa agcgaatccc tggatccggg caggccaggg
    14521 cggctcggat gcaggacggt tcagaaaggc ccacacggca cacgtcaggg ccgcaaaggc
    14581 ggctgcaatc agcagaccag gtacaagctt catgggcaca cacacgcgac gccatgcatg
    14641 atacgcagga cgtgggcctg agcgtaaccg ttttgcagct gtcataagct ccggagccat
    14701 gcccgcgcca gcggcatggt tgccgggagg caggatgcct gcctctggct gaatccaggc
    14761 tgtccggttt accagacccc gacgttgggc agggacttcc acggctcggc cggggcgagc
    14821 gcagccccct tctgcagcag ctcgatcgag atatcatccg gggagcggac aaaggccatt
    14881 cgaccatcgc gcggcggacg gttgatggtc accccgtgct cgcgcaaccg ctcgcaagtg
    14941 gcgtagatgt catccaccgc atacgccaga tgaccgaagt tgcgtcctgc gggatacggc
    15001 tgtggatccc agttgtgagt gagctcgacc tgcgcattct cgtcgtcggg cgcggcgaga
    15061 aagatgagcg tgtagcggcc ctgcggcact tccttgcgcg acagctcctt cagtcccagc
    15121 gcctgacagt agaaccgcag cgacgcctcg acgtcggtaa cgcgaaccat ggtatgcagg
    15181 tacttcatgg ctaaaacatc tccgatgccc cgggcgtctt gatctgcacc ttgcgacggg
    15241 aagggatgaa atccccggtg atcatctcat cataactctg gcccgggccc accatcggat
    15301 acacacccgc atccggatcg atcatcacct cgaggaaagc aggcccatcg aagtggatga
    15361 gctcgtcgat cacccgcggc acgtcctcct tccgatcgag gcgcacggcg taatgaaaac
    15421 cgtctgcctg ggcggccttg acgaagtctt tcttgtgcag cgagcggtcg ctggccgaga
    15481 gccgcccctt gtgaaacagc ttttgccact gcttgaccat tccgtcgccg aagttgttga
    15541 gcacgacgat cttgatgggc aactcgtagg tcgtcaccgt ctcgagctca ccgatgttca
    15601 tgcggatgct ggagtccccg tcgatgtcga tcaccagtcg gcctggattg gcgatctgcg
    15661 caccgatggc cgccggcagt ccgaagccca tcgtgcccat gctgccggag gtcagccaca
    15721 gccgcggctg ccggaaatcg caatattgcg ccgcccacat ctgatgctgg cccaccccgg
    15781 tgctgataat ggcgtttcca gcggcgtggc gattgatctc ctcgatgacg tagtaaggct
    15841 ggatgagcgc actggctcgg tcatagttca tcgcatggac acgcttcagc tcggcgagct
    15901 cagcgtgcca ctgccggtag tcggggtgaa gaccctgacg ccggccctgg gcggtaagcc
    15961 gctcgagcgc ctcggcaatg agaccaacat gactccaatg ggcccgcttg accttgttga
    16021 cttcagcccg atcgatgtcg agatgggcga tgcgctgggc cttgggggcg aactttgccg
    16081 gcacacccgc gacccggtca tcgaaccggg cgcccaccgc gatcaggaaa tcgcaatcat
    16141 ccacggcata gtttgcgaag gccgccccgt gcatgcccag catgcgcatg gacagcggta
    16201 gcgtggtgtc caccgcgcct atgcccatga gtgttgtgac gaccggaatg ccgaatgtgt
    16261 cggcaaagtg ggccagggca ctggcggcgt tcgcgttgat cacgcccccg cccgcataga
    16321 tcagcggccg ccgggactgc gccagcattt cgaaaaaggt ggccgtctgc tcctcgccga
    16381 gcgcgcgcgc ggtgagctcc tgcatgcgac gacggtagcc cggaatcggc agcatgccgc
    16441 cgcccaggaa aggcccctcc cagttctgga catccttggg cacgtccacg accaccgggc
    16501 ccggacggcc ggtccgggca atctcgaaag cggtgcgcag cgtggcttcg aggcgctccg
    16561 gctccgtcac caggaagatg tgcttggcaa tggcgcccat gatcgagggg acgggcgcct
    16621 cctggaaagc gtcggtgccg atcgaggcgc gcgctacctg accgcagatc acgacgagcg
    16681 gcaccgaatc cgccatcgca tcacgtactg gcgtaacagt attcgttgcc ccgggacccg
    16741 aggtgaccat gcacacgccg acccggcccg tggagcgtgc gtaaccggcc gccatgaagg
    16801 cggcgccctg ctcgttcgcc ggcacgatca aggggatctg cgcaccgccc tcgcgcacgc
    16861 gctgctcgtt gtagagaaac actgcatcgt aggtgggcag gatggcgccg ccgctgtaac
    16921 cgaagatggc tgtgacacct tcgtccgcca gcacctgcat gatcatgcgg gcgccggaca
    16981 tcctctgccc ggcgagcgga tgcgcaacgg ggcgcggctg gatcaccgta tcgctcatga
    17041 ttcagacgag cctagcagct tgccgtggct acgcaacacc ggcgatctgc gctgcggcaa
    17101 atgccgccac gttgcgccag atgcgcgcga tatggggtca atccgcgggg aatcctttat
    17161 cctccggtgc tctccagcgc aaacccagcc ccgatgcgtc acatcattgc catcctgctg
    17221 caaaacgaag ccggcgccct cacccgcgtc gccggtcttt tctccagccg cggctacaac
    17281 atcgagtcgc tgtcggtcgc cgccacggat gatcccactg tctcgcgcct gaccctcgtc
    17341 accaagggat cggaaggcgt cattcagcag atcgccaatc agctgaacaa gctggtcgat
    17401 gtcgtgagca tcgaggacat gacgcagggc gagcacctcg agcgtgagct gatactcctg
    17461 aagctgcgcg tgcaacccga gcagaccgat gcggtacgag gctacatcgt gcgcaccggc
    17521 ggtcgtgttc tcgaccccgc gacggacggg ttcatcgccg agctgaccgc gagcgaggcg
    17581 gagatcaacg atttcgttgc ccatctgggc aggcaggcgg cgttgctgga ggtcgtgcgc
    17641 agcggtgcgc tgggaatcgg tcgcaacgcc agaccgctgc gggtcgtgcg ctgagagggc
    17701 gaccagttgc caatgacgcg tgcgcgcatc ctgatcgttg atgatctgcc ggagaagctg
    17761 ctcgtttaca gcagtctgct cgatgacatc gatgccgaga tcgtgacggc gcgctctggc
    17821 acggaggcgc tgaagcgcgt gctcgacggc cagttcgccg tcattctgct cgatgtcaac
    17881 atgcctgaca tcgatggcct ggaaacggca accctcatcc gcagccaccg caacgggcgc
    17941 cataccccga tcatcttcat caccagctac gcggacgaga tgcaaaccac gcgggcgtac
    18001 gagcttggcg ccgtggacta tattctgtct cccgtggtcg cctccgtgtt acggaccaag
    18061 gtgcgcgtat tcgtcgatct ctacgaggcg catgaggctc tggcccgctc caaccgcgat
    18121 ctggaatcgc gggtcaagga gcgcacggcg gagctcgagc agctcagtcg cctcaaggac
    18181 gagtttctcg cgacgatgtc tcacgagctg cggaccccgt tgaacgccat cttcggttgg
    18241 gtgacgttgc tgcgcacacg acggctcgat gccccgacgc aggagcgcgc cctcgagacc
    18301 atcgagcgca acgcccgggc tcagaagcgc ctcatcgagg atctgctcga tgtctcgcgc
    18361 atcgtgaccg gaaaggttgc cctggagctg gcaacctttg acccgcgccg cgcaattcgc
    18421 ggtgccctcg atacgatgca gcctgcggcg caggccaagg acatccggat catctcgagg
    18481 ctggattccg aggtcggcac ggtccgcggc gactttgcgc ggctgcagca gatcgtctgc
    18541 aacctgctgt cgaactccat caaattcacg cagccgggtg gtgagatcga agtctcgctc
    18601 acgccgttca atgagtcggc tcagatctgt gtcaccgaca gcggccaggg catcaagccg
    18661 gaatttctgc cgctggtgtt cgatcgcttt cgccaggagg atggctccat cagccggcgc
    18721 catggtggcc tgggcctggg cctcgcgatc gtgcggcacc tcgtcgagct ccacggaggt
    18781 gccgtggaag cccacagtga cggcgaggga aaaggctcgc gtttcatcat ccggattccg
    18841 acgcgcgcgc agatcggcga cctcgcaggg gccgaggaca cttcccccgc ccccgcttcc
    18901 tcctcgatgc tggcgggcct gcgcctgttg gtcgtggatg acgagcctgg ggcccgcgag
    18961 ttgatctcga atgtgctgcg cggctatggc gctgaggtga gcgtggccga cagcggtcag
    19021 ctcgcgctca cgaagctttt cgaggtgcgg ccgcacgtgc tgatcgccga tctcggcatg
    19081 ccgggcatgg atggctatgc gctcatcgag caggtgcgcg cgctggagcc ggagttcggg
    19141 ggccagacgc ccgccatcgc cgtgaccggc tatgcctcac cctcggatcg cctgcgcgcg
    19201 ctgcaggccg gctaccagaa tcacgtagcc aagccggtcg agccggagga gctggcgatc
    19261 gtgatcagta gtttgagcga gcgaaagcca cgcctttcgt gagcgggtcg gaagcgccgc
    19321 aggcgacttc cggcaatcgg accagctgcg agcgaaagcc acgcctttcg tgagcgcgtg
    19381 ggcgtcgcgt cacggcagca gtacggttga gccggtcgtc cgtctcgcct ccaggtccgt
    19441 ctgggctcgg gccgcttcgg ccagcgggta ggtctgtccg acccgcaccc gcaccgcccg
    19501 gctcttgacc gcggcgaaca actccttcgc agcggcttgc agctcggcac gcgtggaggt
    19561 gtagttgaac agggtcgggc gggtcagaaa cagcgagcca cgcttgctca gctccagggg
    19621 agcgatcggt ggcaccggac ccgaggcgtt gccgaagctg accatcattc ccaggggacg
    19681 cagactgtcc agggactgaa agaacgtgtc cttacccacc gagtcgtaca cgaccgccac
    19741 gcccgctccg cgggttatct gctttacacg cgcgacgaga tcctccttgc ccaggaggat
    19801 gacgtgccgg cagccattct tgcgggccag atccgctttc gcctcgctgc cgacgactcc
    19861 gatgaccttc gcgcccagcg ccttggccca ttgacacagg agcaggccga caccgccggc
    19921 ggcggcgtgc accaggatgt cctcgcctcg gcgcactcga taggttcgcc gcagcaggta
    19981 atgcgccgtg aggcctttca acatgatggc ggccgcctgc tggtcggaaa cccctttcgg
    20041 aatcttgacc agtctgtcgg ccgggacatt acgcacatcg cagtaagccc cgggtcgcgg
    20101 caatacgtac gcgacccggt caccgacttt gaagccgcgc accttgcgcc cgagggccat
    20161 cacgactccc gccgcttccc ggccgatgcc gctgggcaga ctcgtggggt acaggccggt
    20221 acggtcgtag acatcgatga aattcaaccc gaccgccgtc tgacgtaact gggcttcctc
    20281 cggcccaggg ggtccgacgt ccatctcatc cagacgcagc acctcggggc cgccgacctc
    20341 gtgaaatcga atgacccggg cccgcgtgga agcgctcatg ggccgatcac agcgccgcgt
    20401 cgtccgtctc gccagttcgg atccggatca cccgctcgat gtcggtgatg aagatcttgc
    20461 cgtcaccgac cttgccggtc ttggcggact tgagaatcgc ctcgatcacc tggtcgacga
    20521 ggtcgcgccg aacggcgacc tcgatccggg tcttgggaac gaagtcgacc acgtactccg
    20581 cacctcggta gagctccgtg tggccgcgct ggcgcccgaa gcccttcacc tcggtgacgg
    20641 tcatgccgga tacgccgatg tccgagagcg cagcgcgcac gtcatcgagc ttgaacggtt
    20701 tgatgatcgc ggtgaccagt ttcatgggtt gtgctcgtca gcaggccagt cctgcgggtg
    20761 tttcaccgga gtgtaaatca ggcgcggcac ctgcgctgct ccctggaggg ccgaatcgct
    20821 cgcgcgcacc attctggcgc ctgatcgccg tggcgcccgt ccggcagcat gccggccagg
    20881 gtatcggcta tgctttcccg cgtgtttgga cagatcgtca tcgtgggtgc cgggcaagcg
    20941 gccgaccagg ccatccacac cctgcgccgc aagggcttcg ccgggaagct gctactggtg
    21001 ggcgatgagc cctggctgcc ttaccagcgc cccccgttat ccaagaagtt tctggcaggg
    21061 actctcgagc gcgagcgcct cgggctgcac ccggcccaat tctacgttga gcatcaggtc
    21121 gaaacgcgcc tcgggcagcg tgtgcagcag atcgaccggg gtgcgaggcg catccgcctg
    21181 gacgacgaat ccgttgtgcc ctatgacgcg ctgctgctgg cgaccggaag caagcctcgc
    21241 gcactcagcg tgccgggcgc ccatctgccg ggcgtgcatt cacttcgcac cctcggcgat
    21301 gtcgatcgca tacgggccga tatggcggcc ggcgggcgtc tggtcatcgt cggcggcggt
    21361 tacatcgggc tggaggttgc cgcgaccgca cgcgagctgg gcatgcaggt cacggtcatc
    21421 gagatggcgg atcgagtcat gaaccgggtg acctgcaagg agatctcgag cttctacgag
    21481 tccgagcact cgcgccatgg cgtgcggatc atcagcaatg cgcgggtgca ggcgctcgcc
    21541 ggtgactccc gcaccggacg tgtcagagcc gtcctcaccg aagacggcgt ggagcatcca
    21601 gccgacctcg tcattgcggg ggtcggggtg gtcgctgccg atgagctggc cctctcagcg
    21661 ggattggaat gctccaacgg catcgtggtc aacgagtatt gcgcgacgtc cgatcccgcc
    21721 atctttgcgg ccggggactg tacgaatcat ccgaatccac attacggcag gcggctgcgg
    21781 ctcgagtcgg tcgacaatgc ctacgagcag gccgcaagtg ccgcgctcaa cctgctcggc
    21841 gtaccgactc ctcacgacaa ggtgccctgg ttctggtccg atcagttcga cctgaagctc
    21901 atcattgtcg ggatctgtca gggccatgac acggtcatcc tgcgcggcct gccggcaggg
    21961 cgcagcttca gcgcctgtta tctgcgccag ggagagctga tcgccgtgga cagcgtcaac
    22021 agcgcaaagg atcagatggc cgcacgcaaa ctgatcgctg cacgcgcaca tccggatccc
    22081 ggcaaactcg ccgatccagg gatcctgatg agggattgcg cctgatcagg ccaacaccag
    22141 cagaagatcc ttggcatcga cctgctggcc tggctgcgcg agaacctcga cgacttcgcc
    22201 atcgacctct gcgcgaacgg ccgtttccat cttcatggct tcg
//
LOCUS       DASRHN010000275         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2400474, whole genome shotgun sequence.
ACCESSION   DASRHN010000275 DASRHN010000000
VERSION     DASRHN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400474"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="hrcA"
                     /locus_tag="VFB37_11115"
     CDS             complement(<1..940)
                     /gene="hrcA"
                     /locus_tag="VFB37_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZO23041.1"
                     /translation="MGHEERDTETLGERAQHLLRVLIESYIRDGQPVGSRVLSRDSGL
                     QLSSATIRNVMADLEEFGFVSSPHTSSGRVPTDKGYRFFVNTLLRVKPVEEAAIAEIR
                     RQVDSNRDSSKELISSVSQLLSSVTQLAGVVTVPRTQQASITQIEFVALSENRVLVVL
                     VFNDREVQNRIIQLERYYSPDELKRASNYLNEQFRGRTLQQVRHEILRQLQEAREHMN
                     QIMVDAIAVAQKVFEAPPESEERIEYVIAGETNLMGVAELTSVERLRRLFEAFNEKRD
                     FLHLLDQSLRSERIQIFIGHESGYQVLDDCSVVTAPY"
     gene            1205..2035
                     /locus_tag="VFB37_11120"
     CDS             1205..2035
                     /locus_tag="VFB37_11120"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZO23042.1"
                     /translation="MAESAGALIPHLRSREVRVLISEETPFGLDVAVERVPEQAMGAQ
                     VDLMIAIGGDGTLLYAAGLVARHGVPLLGVNRGRLGFLTDVMPQDMFPCIDTALLGKL
                     EADERPLLSSRLQSASGVVSEALALNDVVMQKVATGRMLDFETYLNGTYVNTHAGDGV
                     VVASATGSTAYALSCGGPIIEPHLDAFVVAPICPHTLSDRPIVVSSRSRIEVKLLERP
                     DTRAQITCDGIVLGEFAPGDRLAIQAGAERVTLLHPAGFDYYRLLRSKLHWGRGSFER
                     "
     gene            2046..3899
                     /gene="recN"
                     /locus_tag="VFB37_11125"
     CDS             2046..3899
                     /gene="recN"
                     /locus_tag="VFB37_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZO23043.1"
                     /translation="MLNYLQIRDFAIIDSVELELRPGLTVLTGETGAGKSILVDALQL
                     LAGGRAGAEVVRHGAERAEIIGTFDLSKCPRELKRWLEEQSIYAPEELSIRRVVSSDG
                     RSRAYLNGQAVAVQLLREAGNILIDIHGQHEFQSLTRAAAQRELLDGFGRLEQATGQV
                     GIAHRVWLELLNRTLEIESKTRDRDAKLESLRYQVAELNALQLKPGELLRLAEERARL
                     ANRGRLAEATQMALQLLYEGEEGSAHAGASRALQALRGLANVDSKLESVLPLVEGAAI
                     QIREAAHELTRYRDSLELDTARQDQVERRLAAIEEVARKHRVQPPELAERSAQLITEL
                     EAVERAETDLAVLRKDLGTALENFRTLANQLSAKRTTAGRVLSKDITTRMQTLGMSGG
                     RFQVEVAQDGSAEPTQHGVDQIDFRVTANPGQPLRPLSKVASGGELSRLSLAVQVACA
                     SRDTRCMVFDEVDAGIGGAVAEIVGRELRGLGERGQVLCVTHLPQVASQGHHHLRVTK
                     LTDGRTSRTALSELAPQERIEELARMLGGVEVTARAREHAREMLKAAPQPVADAGATT
                     RISVNPAIEASREAALEATRSAPARSGTAPTPAAPEQTEEEETARPASRRR"
BASE COUNT          667 a         1323 c         1345 g          667 t
ORIGIN      
        1 catagggcgc ggtcacgacg ctgcagtcgt cgagcacttg gtagccggat tcgtgcccaa
       61 tgaatatctg aatgcgctcg gacctcaagc tctggtcgag gaggtgcagg aaatcgcgct
      121 tctcgttgaa cgcttcgaac agccgccgca gccgctcgac gctcgtgagc tcggcgacgc
      181 ccatgaggtt cgtttccccg gcgatcacat attcaatgcg ctcttcggac tccggcggcg
      241 cctcgaacac cttctgcgcc accgcgatgg cgtccaccat gatttggttc atgtgctcgc
      301 gggcttcctg caactggcgc aggatctcgt ggcgcacctg ctgcagggtg cggccccgga
      361 actgctcatt cagataattc gaggcccgct tgagctcatc gggggagtaa tagcgctcga
      421 gctgaatgat gcggttctgc acttcgcggt cgttgaaaac gagcaccacc agcacccggt
      481 tctcggacag ggccacgaac tcgatctggg tgatggaggc ctgctgcgtt ctcggcacgg
      541 tcacgacgcc agccagctgc gtgacgctcg agaggagctg agagacgctc gagatgagct
      601 ccttcgagct gtcgcggttg ctatccacct ggcgccggat ctcggcgatc gccgcctcct
      661 ctaccggctt cacacgcaac agggtattga cgaagaaacg gtagcccttg tcggtcggca
      721 cgcgcccgga agaggtatgc ggcgaggaga cgaacccgaa ctcctcgagg tccgccatga
      781 cgttgcggat ggtcgccgag ctgagctgca ggcccgagtc ccgcgagagc acgcgtgagc
      841 caaccggttg cccgtcgcgg atgtaacttt caatcagcac ccgcagcagg tgttgagcgc
      901 gctcgcccag agtctccgtg tctcgttctt cgtggcccat ggcctgccga tagccgcgtt
      961 gctttgcatc caaaactgcc agttggcggc ccgaacgcta gcgacggcag cccccaccgt
     1021 caagcgatcc ccggcgcaag ccgatgtgaa cgggcacccc cgccaccgcc ccgggcgcat
     1081 tggccctgcc atggcatcgt gctacaaaga cccgcctcgc acgctcgcga ggccccgaac
     1141 gaggctgcag atgggctttc ccgtgcgagt gtgcgcgctg gtcggcagat tccaggaccc
     1201 gcgtgtggct gaatccgcag gcgcgctgat cccgcacctg cgctcccggg aggtccgggt
     1261 gctgatcagc gaggagaccc ccttcggcct ggatgtcgcg gtcgagcggg tgcccgagca
     1321 ggcgatggga gcccaggtgg acctgatgat cgccatcggt ggggacggca ccctgctgta
     1381 tgcggcgggc ctggttgccc ggcacggggt acccctgctc ggtgtcaacc ggggacggct
     1441 cggcttcctc acggacgtga tgccgcagga catgtttccg tgtatcgaca cggcgctgct
     1501 cggaaagctc gaggccgatg agcggccgct gctctcctcc cggctgcaga gcgcctccgg
     1561 ggtcgtgtcc gaggcactgg cactgaatga tgtggtgatg cagaaggtgg caacgggccg
     1621 catgctcgat ttcgaaacct acctgaacgg gacgtacgtc aacacgcacg caggcgacgg
     1681 cgtcgtggtg gcgagcgcta ccggctctac tgcgtacgct ctctcgtgcg gcggtccgat
     1741 catcgagccg cacctggatg ctttcgtggt cgcccccatt tgcccgcaca cgctgtcgga
     1801 ccggcccatc gtcgtctcgt cgcgatcgcg cattgaagtc aagctcctgg agcgtccgga
     1861 tacgcgcgcc cagatcacat gcgacggaat cgtgctcggg gagtttgcgc ccggggaccg
     1921 gctggccatc caggccggcg ccgagcgcgt gacgctgctg cacccggccg gtttcgacta
     1981 ctaccgcctg ctgcgctcca agctgcattg gggccgcggc agcttcgaac gctgaggtcc
     2041 atcacatgct caactatctg cagatccggg acttcgcgat catcgatagc gtcgagctgg
     2101 aactgcggcc cggactgacg gtgctgaccg gcgagactgg cgcgggcaag tccattctgg
     2161 tggatgcgct gcagttgctg gccggggggc gcgcgggcgc cgaggtggta cgccatggcg
     2221 ccgagcgagc cgagatcatc gggaccttcg atctgagcaa atgcccgcgg gagttgaagc
     2281 gctggctgga ggaacaatcc atctacgctc ccgaggagct ttcgatccgc cgcgtggtct
     2341 cgagcgacgg tcgctcccgc gcgtatctga acggccaggc cgtggcggta cagctgctgc
     2401 gcgaagcggg caacatcctg atcgacatcc acgggcagca cgaatttcag tcgctcaccc
     2461 gggcggccgc gcagcgcgag ctgctggatg gattcggccg cctcgagcag gcgaccggcc
     2521 aggtcggaat tgctcatcgc gtctggctcg agctcttgaa ccgcacactg gagattgaat
     2581 ccaagacacg cgatcgcgac gccaaactcg aatcgctgcg ttaccaggtc gccgagctga
     2641 atgccttgca gctgaagccc ggcgagctcc tgcgcctcgc cgaggagcgc gcacgcctgg
     2701 ccaaccgcgg ccggctcgcg gaagcgaccc agatggctct tcagctgctc tacgagggtg
     2761 aggagggcag cgcgcatgcc ggcgccagcc gcgcgctgca ggcgctgcgc ggcctcgcca
     2821 atgtggactc caagttggaa tccgtgttgc cgctggtgga gggtgccgcg atccagatcc
     2881 gtgaggccgc ccacgagctc acccgctatc gcgacagcct cgagctcgac accgcgcgcc
     2941 aggatcaggt cgagaggcgg ctcgcggcga tcgaggaagt ggcccgcaag caccgcgtgc
     3001 agccgccgga gcttgccgag cgctccgcac agctcatcac cgagttggag gcggtagagc
     3061 gtgcggagac ggatctcgcc gtgttgcgca aggacctggg gacggcgctg gagaacttcc
     3121 gcaccctcgc caaccagctt tcggcgaagc gcacgaccgc aggccgtgtg ctgtccaaag
     3181 acatcacgac gcgcatgcag actctgggaa tgtccggcgg ccgctttcag gtcgaggtgg
     3241 cgcaggatgg ctccgccgag ccgactcaac acggcgtcga tcagatcgac ttccgcgtaa
     3301 cggccaatcc gggccagccg ctgcgaccgc tttccaaggt ggcctcgggc ggtgagctct
     3361 cgcgcctgtc gctggccgtg caggtcgcct gcgcctcccg cgacacgcgc tgcatggtgt
     3421 ttgatgaagt ggacgccgga atcggcggcg cggtggcgga gatcgtcggc agagagctgc
     3481 gcgggctggg cgagcgcggc caggtgttgt gtgtgacgca tctgccgcag gtcgcatccc
     3541 agggccatca tcacttgcgc gtcacgaagc tcaccgatgg ccgcacgagt cgtaccgcgc
     3601 tctccgagct cgccccccag gagcgcatcg aggagctcgc ccgcatgctc ggcggcgtcg
     3661 aagtgaccgc gcgcgcccgt gagcatgccc gcgagatgct caaggcagct ccgcagcccg
     3721 tggcggacgc gggagcgacc acacggatca gcgtgaatcc ggccatcgag gcgtccaggg
     3781 aagctgcctt agaggctacc cgcagcgcgc ctgctcgttc cggcaccgcg cccacgccgg
     3841 cggcgccgga gcagactgag gaagaagaga ccgcgcgccc tgcctccagg cgccgctgaa
     3901 cgcccgaatc ggctcagctc gccttcttct tgcggtaggc gcagttggtg cggcggcagt
     3961 ggccgtacat gatcagggaa tgatcgcgaa tggtgaatcc ca
//
LOCUS       DASRHN010000276         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_587918, whole genome shotgun sequence.
ACCESSION   DASRHN010000276 DASRHN010000000
VERSION     DASRHN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587918"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1076)
                     /gene="cysB"
                     /locus_tag="VFB37_11130"
     CDS             complement(105..1076)
                     /gene="cysB"
                     /locus_tag="VFB37_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HZO23044.1"
                     /translation="MKLHQLRYLAAVAQSGLNITAAAQKLHTSQPGVSKQIKLLEDEL
                     GFQIFVREGRNLTRITPAGQQVIERALKILQEAESIRDLSTELRDEGRGSLSIGTTHT
                     QARYVLPSVISEFRNRYPNVRLNLHQGTSEQIAEMVAHDRIDCAIATGSDHLYSDLTL
                     MPCYRWHRTVIVPHDHPLASAGRLTFKALAAYPLVTYTFSFTGPSSLHEAFAKAGLTP
                     NVAITARDADVIQTYVRLGLGVGIVAHMAIEPNDPDLTSIEAGHLFPAHTTWIGFRRG
                     TLLRKYMYDFAQLLAPHLDRRLVDRAHRAATPEETAELFKDIELPTR"
     gene            1208..1927
                     /locus_tag="VFB37_11135"
     CDS             1208..1927
                     /locus_tag="VFB37_11135"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZO23045.1"
                     /translation="MGELLNLDLEHKAASVRQQLVEAVREHGRVIYSNSLGAEAMVLT
                     DIIWSHVPEIDIFSIDTGRLHEETYELLEKLQRRYKRSIRLVYPDAQALEKLVADQGV
                     NGFYHSLEARLACCHVRKVEPFRRAVAGYSAWVTGVRREQSATRAQAQPIEWDTENSM
                     HKVSPLLDWTEEQVWQYIRARKLPYNSLHDRQFPSIGCSPCTRAIQPGENRRAGRWWW
                     EQPESRECGLHPRVRHAAVQA"
     gene            1945..>2752
                     /locus_tag="VFB37_11140"
     CDS             1945..>2752
                     /locus_tag="VFB37_11140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO23046.1"
                     /translation="MDHLPVFLQLRARPAVVVGGGQVAARKIDLLLRSGARVTVIAPE
                     LLEELRKPAAEGLFRYIPASFDDSHLDEAELAVAATDLREVNLAVSEAARRRRIPVNV
                     VDDPQLSTFIFPAIIDRSPIVVAVSSGGQAPVLTRRLREQLEALLPARLGNLARFMGE
                     RRKAVQIALRRAARRPFWERIVAGLTTSRVLAGDEPGARQAFDRELYTSQLTDSAATG
                     GSGLGEVYLIGAGPGDPDLLTLRALQLLQQVDIVLYDRLVPDAVLDRARRD"
BASE COUNT          467 a          900 c          903 g          482 t
ORIGIN      
        1 tcgaactgct cgaaataaag tcccgccatg aaaggatctc cgtgacccgc cgcgcgtaag
       61 gagccgctgc gcgggctgtc tgagccgccg cagcggcggc acttctacct ggtcggcaac
      121 tcaatatcct tgaacagctc cgccgtctcc tcgggcgtcg cggcccggtg cgcccggtcg
      181 acgagccggc gatccaggtg cggtgcgagc aactgcgcga agtcgtacat gtacttgcga
      241 agcaacgtgc cgcgccggaa gccgatccac gtcgtgtggg ctggaaacag gtgccccgcc
      301 tcgatggagg tcaggtcggg atcgttcggc tcgatggcca tgtgagcgac gatcccaaca
      361 cccaggccca gccgcacata agtctggatg acatcggcgt cccgcgccgt aatggccacg
      421 ttcggcgtga gacccgcttt cgcaaacgcc tcatgcaagg aggacggccc ggtgaagctg
      481 aaggtgtacg tgacgagggg atacgcagcg agggccttga acgtgagccg acccgccgag
      541 gcgagcgggt ggtcatgcgg gacgatcacc gtccgatgcc agcgatagca gggcatcagc
      601 gtgaggtccg agtaaagatg gtcggagccg gtcgcgatgg cgcagtcgat ccggtcgtga
      661 gcgaccatct cggcgatctg ttccgaagtg ccctggtgca gattgagccg cacgttcggg
      721 tagcgattgc gaaactcgct gatcacgctt gggaggacgt accgcgcctg ggtatgcgtg
      781 gtgccgattg acaggctccc ccgcccctca tcgcgcagct ccgtggaaag gtcacggatg
      841 ctctccgcct cctgcaggat cttcagcgcc ctttcgatca cctgctggcc ggctggtgtg
      901 atgcgcgtca gattccggcc ctcgcgcacg aagatctgga agccgagctc atcctcgaga
      961 agcttgatct gcttgctcac ccccggctgg gaggtatgca gcttctgggc agccgccgtg
     1021 atgttcagcc cgctctgggc cacggccgcg agataccgca attggtgcaa tttcatgggc
     1081 ctaagcgtag cgcaagcgcg tgcgggccgg gtcttttccc tgtaaccgcc ttataaccgg
     1141 cccgaagtgg ctcagtagca atggttcgtt ctgccccccg aggggggtgg ctatagtcgc
     1201 ccgcaccatg ggagaactcc tgaatctcga tctagagcac aaagccgcct cggtgcggca
     1261 gcagctcgtg gaggcggtgc gggagcacgg gcgcgtgatc tactcgaaca gcctgggcgc
     1321 cgaggccatg gtgctgaccg atatcatctg gtcgcacgtc cccgagatcg acattttcag
     1381 catcgatacc ggccgtctcc acgaggagac ctacgagctg ctggagaagc tgcagcgccg
     1441 ctacaaacgc agtatccgcc tggtctaccc cgacgcgcag gcgctcgaga aactcgtggc
     1501 cgaccagggc gtcaacggct tttaccacag cctcgaggca cgcctcgcgt gctgccacgt
     1561 acgcaaggtt gagcctttca gacgggcggt ggcgggctac tccgcatggg tgaccggcgt
     1621 ccgccgcgag caatcggcta cgcgtgcgca ggcgcagccg atcgagtggg acaccgaaaa
     1681 cagcatgcac aaggtcagcc ccctgctgga ctggaccgag gagcaggtct ggcagtacat
     1741 ccgggcccgc aagctaccgt acaattccct gcacgaccgc cagtttccga gcatcggctg
     1801 ctcgccctgc acccgggcga ttcaacccgg cgagaaccgc cgcgccggcc ggtggtggtg
     1861 ggagcaaccc gagtcgcgcg aatgcggtct tcacccccga gttcgtcatg ccgccgtgca
     1921 ggcctgagcg gcgccacggc atccatggat catctgccgg ttttcctgca gctgcgcgcc
     1981 cggcctgcgg tcgtcgtggg cggcggccag gtggccgcgc gcaagatcga cctgctgttg
     2041 cgcagcggcg cgcgggtcac ggtcatcgct ccggagctgc tcgaagagct gcgtaagccc
     2101 gccgcggagg ggcttttccg gtacattccc gcgtccttcg acgattctca cctggatgag
     2161 gccgagctcg ccgttgcggc gactgacctc cgtgaggtca acctcgcggt gtcggaagca
     2221 gcgcgcaggc ggcgcatccc ggtcaatgtc gtcgatgatc cgcaactgtc gaccttcatc
     2281 tttccagcca tcattgaccg ctcgcccatc gtcgtcgcgg tgagttcggg cggccaggcc
     2341 ccggtactga cgcgcaggct gcgcgagcag ttggaagcac tgctgccggc gaggttgggg
     2401 aatctcgctc ggttcatggg tgaacgacgt aaggctgttc agatcgcatt gcgccgtgcg
     2461 gcgcgccgac ccttctggga gcggatcgtc gctggactga ccacctccag agtgctggcg
     2521 ggcgatgagc ccggcgcccg acaagccttc gatcgcgagc tctacacctc tcagctcact
     2581 gactcagcgg ccaccggcgg cagcggcctc ggcgaggtct acctcattgg cgcgggcccc
     2641 ggcgatccgg atctcctgac actgagagcc ctgcagctgc tgcagcaggt cgacatcgtg
     2701 ctttatgacc ggcttgttcc cgacgccgta ctcgaccgcg cacgccggga tg
//
LOCUS       DASRHN010000277         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_587946, whole genome shotgun sequence.
ACCESSION   DASRHN010000277 DASRHN010000000
VERSION     DASRHN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587946"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFB37_11145"
     CDS             <1..416
                     /locus_tag="VFB37_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZO23047.1"
                     /translation="VPFSAMMTRVLTVEAGGQMYGVPLESVIETVSVPTAALARVGAA
                     EAIVLKGRTIPVLDLASLLGARPQPKEAAEATIVVVAFAGQNGGLRVDRLGESLEVML
                     RPCEGLLLGTPGITGTTLMGDGRVLLVLDIAEMLQ"
     gene            737..1120
                     /locus_tag="VFB37_11150"
     CDS             737..1120
                     /locus_tag="VFB37_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO23048.1"
                     /translation="MTHTVLIVDDSKLARMAVIKILDSLHPDWARVEAANAEEALQRV
                     RQGAADIALVDFNMPGKDGVLLAAEIRRLNLGISVAVISANHQIEVVNRAQSAGAVFL
                     RKPLTESALGDFLNAAAQQHEARKS"
     gene            1117..1776
                     /locus_tag="VFB37_11155"
     CDS             1117..1776
                     /locus_tag="VFB37_11155"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HZO23049.1"
                     /translation="MRQTATIVLSELELDALTELANLGVSVAATSLREMAREEVMLSV
                     PKVAVVTREEAIANLGERESRRLVAVHQDFEGAISGRAFLIFPEARSMELVRAVVGGD
                     LSLEEITELEQEALAETGNILLNGCLGTIANNLERSLRISLPEVVHGEGTEFFSLGRI
                     PTAGDSVLFIYINFAVRQRDIQGYIAMLLDLPSLAALKELLVAFIDRTTGTRSALPHV
                     P"
     gene            1766..>3931
                     /locus_tag="VFB37_11160"
     CDS             1766..>3931
                     /locus_tag="VFB37_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO23050.1"
                     /translation="MSLSASSEAVALLDAINGGIVVLDRSERVIRWNAWMESATGCAE
                     SEVRGKSVADIFPHVDMKRLAPAIKAALQSGAATILTHALHPSLLPLFSRSRQPLLHN
                     ITVSPVGHEAITACLVEVADVSMAIKRERYLREQQNARYDAVVASAPDVIMTVDEAGI
                     IQFVNPAAISQFGYSNSELVGTDVGLLLETKAEWTASWRGVLASAAPIESRQLVGRRK
                     DGSSTYLEASASRWKSGTRNYLTVILRDVNDRRAKDAALRASEEQARRAAAALTDLNQ
                     TLEQRVQDRTAQLMKAEEALRHSQKMEAIGNLTGGIAHDFNNLLQVISGNLALLKHDV
                     AGNALAQRRVQNAMDGVIRSGKLSSQLLAFARRQALEPKVINLGRFIRDMEDMLRKAV
                     GEGVTVEMLVAANLWNTLIDPGNVENALLNLAINARDAMDGQGRLTIEVANAFLDSDY
                     TEEHAEVIRGQYVLITVTDTGRGMPPEVIEQAFEPFFTTKPEGRGTGLGLSMVYGFVK
                     QSGGHIKIYSEVGQGTTIRLYLPRSMQSEDRLVNFDAMPIKGGSEVVLVAEDDESVRE
                     TVVAMLSDLGYRVLKAKDAHSALSIIESGMPIDLLFSDVVMPGPLKSSELARKARERM
                     PNLAVLFTSGYTENSIVRSGRLQENVELLSKPYTREALARKLRHVLEIAAQRQYPYSS
                     NAVAPVPSPRNRLRVLVCESDASIRESTVEMLRTMGHQPV"
BASE COUNT          746 a         1206 c         1265 g          714 t
ORIGIN      
        1 cggtgccgtt cagcgccatg atgacgcggg tgctgacggt cgaggccggc ggtcagatgt
       61 atggggtccc actggagagc gtcatcgaaa ccgtgagcgt cccgacagca gccctcgcac
      121 gggtcggggc tgcggaagcg atcgtgctca aaggtcgcac cattcccgtg ctcgatctgg
      181 cgagcctgct gggggcaagg cctcagccca aggaggctgc cgaagcgacc atcgttgtcg
      241 tcgctttcgc aggacaaaac ggcgggctcc gcgtcgatcg cctcggggag agcctcgagg
      301 tcatgctcag gccctgcgag gggctgctgt tgggaactcc cgggatcacc ggcaccacgc
      361 tgatgggcga cgggcgcgtc ctcctggtcc tggacatcgc cgagatgttg caatgagcgt
      421 ttctttatcg gccagcgggg cgattaccct cgaaggggcc tgctcgagtg aggatgctga
      481 ggtcctcctg cagcacctgc tgggcgcgcc cgcctccatg gtcgattggc gggcgtgcga
      541 gtcggcacac acggctgtca ttcaggtgct actggccgcc cgaccgggac tgctgggccc
      601 gcctctgaac ccgcaattgg cgcaatgggt gcaaccgctt ctagcccccc aatcggcgtc
      661 caatgaggca acttaggtct gctaccatgg gatttcactt gcgggggttt ccggtcaccg
      721 acgactagga gggttcgtga cgcacaccgt cttgatcgtg gacgacagca agctggcccg
      781 catggcggtc atcaagattc tcgattccct gcaccccgac tgggcgcggg tcgaggcggc
      841 aaacgcggaa gaggcgctgc agcgggtccg acagggtgct gcagacattg cgctcgtgga
      901 cttcaacatg cctggcaagg acggcgtgct cctggcagcc gagattcgcc gactcaatct
      961 aggcatcagc gtcgcggtga tctccgccaa ccatcagata gaggtcgtca atcgggcgca
     1021 atcggccggc gcggtgtttc tgcgcaagcc cttgaccgaa tcggcactag gtgacttcct
     1081 gaacgctgcc gcgcaacagc atgaggcaag aaagtcatga ggcaaaccgc aaccatcgta
     1141 ctgagcgaac tggagctgga tgccctgacg gaactggcga atctgggtgt cagcgtcgca
     1201 gcaaccagtc ttcgggagat ggcgcgcgag gaagtcatgc tgtcggtgcc caaggtcgcg
     1261 gtggtgacgc gtgaggaggc aatcgccaat cttggggagc gggaatcccg gcgcctcgtg
     1321 gccgtgcatc aggatttcga gggagcgatc agtggccgcg cgttcctgat cttcccggag
     1381 gcccgcagca tggagctggt gcgtgccgtg gtgggcggag atctgtcgct cgaagagatt
     1441 accgagctgg aacaggaagc actcgccgaa accggcaaca ttctgctgaa cggctgcctg
     1501 ggcacgattg ccaacaacct ggagcgcagc ctgcggatct ccttgccgga agtggtgcac
     1561 ggtgaaggaa cggagttctt ctctctggga cgcatcccca cggctggaga tagcgtcctg
     1621 ttcatctata tcaactttgc cgttcgacag cgcgatattc aagggtacat cgccatgctg
     1681 ctcgacctgc cctcgcttgc ggctctgaag gaactgctgg tggcgttcat cgaccgcacg
     1741 accgggacgc gatctgccct gccccatgtc ccttagcgcg agctccgagg ccgtcgctct
     1801 gctggatgcg atcaacggtg ggatcgtcgt cctggatcgc agcgaacggg tcatccgctg
     1861 gaacgcctgg atggagtccg ccaccggatg cgccgagagc gaggttcgtg gcaagagcgt
     1921 cgccgacatc tttcctcatg tcgatatgaa gcgcctcgcg cctgccatca aggcagctct
     1981 gcaatcgggc gccgccacga ttctcaccca cgcgctgcat ccctcgctgc ttccgttatt
     2041 ctcacgctcc cgccagcccc tgctgcacaa catcaccgtt tcgccagtcg gtcatgaggc
     2101 gatcacggca tgcctggtcg aagtcgccga tgtgtccatg gcgatcaaac gggagcgcta
     2161 tctgcgtgag caacagaatg cgcgctacga tgcggtcgtc gccagcgctc ccgatgtcat
     2221 catgacggtg gatgaggcgg gcatcatcca attcgtgaat ccggcagcca tctcccagtt
     2281 cggctactcc aattccgagc tggtcgggac ggatgtcggc ttgttattgg agacgaaggc
     2341 cgagtggacc gcctcctggc gcggtgttct tgcgagtgcc gcgcccatcg agtcgcgaca
     2401 gctcgtgggc cggcgcaagg acggttcgag cacctatctg gaggcgtcgg cttcacgatg
     2461 gaaaagcggc acgcgcaact acctgaccgt catcctgcgc gatgtcaacg atcgccgggc
     2521 caaagatgcg gcgctgcgcg ccagtgagga acaagcgcgc cgagcggccg ctgccctcac
     2581 ggatctcaat caaaccctcg agcagcgcgt tcaggatcgg accgcccagc tcatgaaggc
     2641 cgaggaggcg ttgcgccaca gccagaaaat ggaggcgatc ggcaacctca ccggcggcat
     2701 cgcgcacgat ttcaacaatc tgctgcaggt gatcagcggc aatctggcgc tcctgaagca
     2761 cgacgtggcg ggcaatgccc tcgcgcagcg gcgagtgcag aacgcgatgg atggcgtgat
     2821 acgcagcggc aagctctcct cgcaattgct cgcgtttgcg cggcggcagg ctctggagcc
     2881 gaaggtgatc aacctgggtc gcttcattcg cgacatggag gacatgcttc gcaaagcggt
     2941 cggtgaaggg gtcacggtcg agatgctggt cgccgcgaat ctgtggaata cgctcatcga
     3001 tcccggcaac gtcgaaaatg cgctgctgaa cctggccatc aacgcacggg atgccatgga
     3061 cggtcagggc cgcttgacga ttgaagtggc caatgcgttc ctggattccg actacaccga
     3121 agagcacgcc gaggtgattc gagggcagta tgtcctgata acggtcacgg acacgggccg
     3181 cggcatgccc ccggaagtga tcgagcaggc cttcgagccg ttcttcacga ccaagcccga
     3241 gggtcggggt acaggactcg gactgtcgat ggtctacggg ttcgtcaagc agtccggcgg
     3301 tcacatcaag atctacagcg aggtcggcca gggcacgacc atccggctgt acttaccgcg
     3361 ctcgatgcaa tccgaggaca ggctcgtcaa tttcgatgcg atgcccatca agggcggcag
     3421 cgaggtcgtg ctggtcgcgg aggacgatga gtccgtccgg gagaccgtcg tggcgatgct
     3481 cagcgacctt ggctatcgcg tgctgaaggc caaagacgcg cacagcgcac tcagcatcat
     3541 cgagagcggc atgccgatcg atctgctgtt cagcgatgtg gtcatgcccg gccctctcaa
     3601 gagctccgag ctggcacgca aggcgcgcga acgtatgccg aacctggcgg tgctcttcac
     3661 gtccggctac acggaaaact ccatcgtccg ctcgggtcgt ctgcaggaga acgtcgagct
     3721 gttgagcaag ccttatactc gcgaggcgct cgctcggaaa ctgcgtcacg tgctggaaat
     3781 cgcagcccaa cgacaatacc cctactcttc caacgcagtc gctccagtgc cctcgccaag
     3841 aaatcgcttg cgtgtgctgg tatgcgaaag cgacgcctcc atccgcgagt ccaccgtaga
     3901 gatgcttcgc accatgggcc atcagccggt a
//
LOCUS       DASRHN010000278         7005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1741910, whole genome shotgun sequence.
ACCESSION   DASRHN010000278 DASRHN010000000
VERSION     DASRHN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741910"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="dxs"
                     /locus_tag="VFB37_11165"
     CDS             complement(<1..1038)
                     /gene="dxs"
                     /locus_tag="VFB37_11165"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZO23051.1"
                     /translation="MTVTPAQYPLLEAINSPQDLRSLPASKLPELAQELRDFLIQSVS
                     TRGGHFAAGLGTVELTIALHYIFDTPHDRLVWDVGHQAYPHKVLTGRRDQLHTIKQPG
                     GLAPFPSRLESEYDTFGVGHSSTSISAALGMAVASARRGENRSVVAIIGDGAMTAGMA
                     FEALNHAGSLPADLLVILNDNDMSISENVGALSNHFARALSGRVYAHLREGGKKVLRQ
                     MPTVWELARRSEEHLKGMVLPGTLFEELGFNYIGPMDGHDVKALVSTLRNLKKLRGPQ
                     FLHVVTRKGKGYAPAEADPIKWHGPGPFDPVSGTIFKEKSTGPSYSQIFGQWLCDMAE
                     RDPLIVGITPAM"
     gene            complement(1117..2010)
                     /locus_tag="VFB37_11170"
     CDS             complement(1117..2010)
                     /locus_tag="VFB37_11170"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZO23052.1"
                     /translation="MSALPPRIEAYRSRVERVLQQCLSLPDAGTARLREAMQYSVLGG
                     GKRLRPALVYLTGESLGAPLSELDAAAAAVELIHVYSLVHDDLPAMDDDDLRRGRPTC
                     HRAYDEATAILVGDALQALAFAVLADESFGSTSAALRLAMIRTLARAAGTGGMAGGQA
                     VDLAAVGHRLSVPDIENMHRRKTGALIEGSVLLGALGAGVSAGREFQALERYGAEIGL
                     AFQIQDDILDVEGDPALLGKSTGADAALRKPTYPSTVGLSAARERARDLRDRALEALV
                     PLGVRASALAELAQFVVNRAG"
     gene            complement(2030..2317)
                     /locus_tag="VFB37_11175"
     CDS             complement(2030..2317)
                     /locus_tag="VFB37_11175"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZO23053.1"
                     /translation="MPSDPPSSPAPECPDFEQALGELERLVERLERGDLPLEEALRTF
                     ERGVELTRHCQSSLKAAQQKVEILLRRGPHSGVEPFSVPEESADSPAAGSE"
     gene            2463..2876
                     /locus_tag="VFB37_11180"
     CDS             2463..2876
                     /locus_tag="VFB37_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZO23054.1"
                     /translation="MTLKGSKTEKNLKDAFAGESQANRRYLYFAAKADVEGFNDVSAV
                     FRSTAEGETGHAHGHLEYLEAVGDPATGLPIGETKLNLKAAIAGETHEYTDMYPGMAK
                     AARDEGFAEIADWFETLAKAERSHANRFQKALDAI"
     gene            2971..4365
                     /locus_tag="VFB37_11185"
     CDS             2971..4365
                     /locus_tag="VFB37_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZO23055.1"
                     /translation="MSSEGAPSEGQSGSGTGGREGSLEAPTRHPIDWRNPQFYDEAAV
                     TRELERVFEICHGCRRCFSLCNSFPTLFDAIDASKTMELDGVDRKVYWEVVDNCYLCD
                     MCFMTKCPYVPPHPWNVDFPHLMLRAKATHARQTGFSFRDRMLSRTDTVGNIAGLPVV
                     AELVNAVNGTSAGRALLQKTLGVARDAPLPKYYSRTARKRLSDRSDTDSAIADAAAVK
                     ATEQTSGKVALFATCYANRNEPELAEDLAAVLEHNGIAVRLIAQERCCGMPRLELGDL
                     EGVAQLKEFNIPQLKRAVDSGFDLIAPIPSCVLMFKQELPLMFPQDADVRAVQRRIFD
                     PFEYLMLRHRAGLLRTDFKSPLGKISYHVPCHLRVQNMGLKTRDVLKLVPETTIEVIE
                     RCSGHNGTYAVKKEFRAASVKIGRPVVQRIESAKPDHYCSDCPMAGHQLESGLAQPRE
                     PEHPLKLLRAAYGI"
     gene            4373..4957
                     /locus_tag="VFB37_11190"
     CDS             4373..4957
                     /locus_tag="VFB37_11190"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HZO23056.1"
                     /translation="MNKLSSGDLFSLERYARERAAFRAQVLEHKRHRQLPIGPNVTWL
                     FEDRLTIQYQVQEMLRAERIFEPEGIEEELESYNPLIPDGTNWKVTLLIEFPDEQERR
                     QALVRLNGIENRCWVRVSGHETVFAVADEDLERANDEKTAAVHFLRFELSGPMIASLQ
                     GGVGLAAGIDHPHYRHVVELVSGPVRDALTADFA"
     gene            5116..7005
                     /gene="parE"
                     /locus_tag="VFB37_11195"
     CDS             5116..7005
                     /gene="parE"
                     /locus_tag="VFB37_11195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HZO23057.1"
                     /translation="MSQSYTASDIEVLSGLEPVRRRPGMYTHTSRPNHLAHEVIDNSV
                     DEAIAGHCKQIDVTLYKDGSLEVADDGRGMPVDIHPKEKVSGVELILTRLHAGGKFSD
                     QTYRFSGGLHGVGVSVVNALSKHLECWIRRGGKEYNISFKDGKVSSKLEVIGTVGQRN
                     TGTTIRFWPDPQFFDSDKFSVAPLKHTLKAKAVLSPGLRVTFTNEATKEKDEWFFTGD
                     LGAYLLEELGSGEKLPAEPIIGPGEAGNEAVSYALLWAPQAEAVVAESYVNLIPTIEG
                     GTHVNGLRSGVAQAVREFCEFRNLVPRGIKLTPEDIWDKACYVLSVKIQEPQFAGQMK
                     ERLGSRDAAQLVESQVRDAVALWLNRHPDAGERIAQFAISNAQERLKAAQRVVRKRSS
                     SGPALPGKLADCSSQEPRRSELFLVEGDSAGGSAKQARERDFQAIMPLRGKILNTWEL
                     EPGEIGASQEVHDISVALGVEPGSTDLSQLRYHKVCILADADSDGQHIATLLCALFLR
                     HFRPLVANGHVYVAMPPLYRIDAGKQVLYALDDSERDQLLKKVEKENPRTRPSVTRFK
                     GLGEMNPAQLRETTIDPRTRRLVQLTLEGKDNTDQLMDMLLAKKRAADRREWLEQNGN
                     LAEVL"
BASE COUNT         1291 a         2267 c         2301 g         1146 t
ORIGIN      
        1 catggccggt gtgatgccca cgatcagcgg atcgcgctcg gccatgtcgc atagccactg
       61 gccgaagatc tgcgagtacg aagggccggt gctcttttcc ttgaagatcg tgccgctgac
      121 cggatcgaag gggccgggcc cgtgccactt gatcggatcg gcctcggccg gcgcgtagcc
      181 cttgccttta cgcgtgacga cgtgcaggaa ctgcggtccg cgcagcttct tgagattgcg
      241 cagcgtggac acgagcgcct tgacgtcgtg gccgtccatg ggaccgatgt agttgaagcc
      301 gagctcctcg aacagcgtgc ccgggagcac catgcccttg agatgctcct cggagcggcg
      361 ggccagctcc cagacggtcg gcatctgccg cagcaccttc ttgccgccct cgcgcaggtg
      421 cgcatacacg cggcccgaga gcgcccgggc gaaatgattg gagagcgcgc cgacgttctc
      481 cgagatggac atgtcgttgt cgttcaggat cacgagtaga tccgccggca gcgagccggc
      541 atgattgagc gcctcgaagg ccatgccggc agtcatggcg ccgtcgccga tgatggccac
      601 cacgctgcga ttctcaccgc ggcgcgcgct cgcgacggcc atgccgagcg cggcgctgat
      661 cgaggtactc gagtgcccga ctccgaaagt gtcgtactca ctttcgagac gcgaggggaa
      721 aggagcgagt ccaccgggct gcttgatggt gtgcagctgg tcgcggcgac ccgtcagcac
      781 tttgtgcgga tacgcctggt gacccacatc ccacacgagg cggtcatgag gcgtatcgaa
      841 gatgtaatgc agcgcaatcg tcagctcaac ggtgcccaaa cccgctgcaa aatggccccc
      901 gcgcgtgctg acgctttgga tgagaaagtc gcgcagctcc tgcgcgagct ccggaagctt
      961 ggaagcgggc aggctccgca ggtcctgcgg tgagttgatg gcttcgagca acggatattg
     1021 ggctggcgtg acagtcattc gcgtgaagtg tacaccgggc cgcgacagcg cagccctaaa
     1081 ccgccggacc cgccccggcg cccttccagc gcgccgctaa ccggcacggt tgaccacgaa
     1141 ctgcgcaagc tcggccagcg cagaggcgcg cacaccgagc ggcaccagcg cctcgagagc
     1201 ccgatcgcgc aagtcccggg cccgctccct cgcggcgctc aggccgacgg tactcgggta
     1261 ggtcggcttg cgaagcgcgg catcggcccc ggtggacttg ccgagcaggg ccgggtcgcc
     1321 ctcgacatcg agaatgtcgt cctggatctg aaacgcgagc ccgatctcgg caccgtagcg
     1381 ctcgagcgct tgaaattcgc ggccggcgct gacgcccgct ccgagcgccc ccaacagcac
     1441 gctgccctcg atcagtgcgc cggtcttgcg gcgatgcatg ttctcgatgt ccggcacgct
     1501 gagtctgtga ccgacggcgg ccagatcgac cgcctggccg cccgccattc cgccggtgcc
     1561 ggctgcgcgc gccagggtac gaatcatcgc gaggcgcagc gccgcggagg tgctgccgaa
     1621 ggactcatcg gccagcacgg cgaaggcgag cgcctgcagc gcatcgccga ccaggatggc
     1681 agtggcttca tcgtaggcac ggtgacaggt cggacgtccg cgccgcagat cgtcgtcgtc
     1741 cattgccggc aaatcgtcgt ggaccaggct gtagacatga atcagctcga cggccgcagc
     1801 cgctgcatcg agctcgctca ggggcgcacc cagcgactca cccgtgagat aaacgagtgc
     1861 cggccgcaac cgcttgcctc cgccgagcac gctgtattgc atcgcttccc gcagccgagc
     1921 cgtgcccgca tccggtaacg acaggcactg ctggagtacc cgttcgactc gcgagcgata
     1981 cgcctcgatg cgcggcggca aagcgctcat cgtcgtgccg tggctgcgct cactctgacc
     2041 cggcggcggg cgagtcggcg gattcctccg gcacgctgaa aggctcgacg ccgctgtgag
     2101 ggccccgcct gagcaggatc tccaccttct gctgtgcggc tttgagcgag gactggcagt
     2161 ggcgcgtcag ctccacgccc cgctcgaagg tccgcagcgc ttcttccagc ggcagatcgc
     2221 cgcgctcgag ccgctcgacg aggcgctcga gctcgccgag tgcctgctcg aaatccgggc
     2281 actctggagc gggagaggat ggcgggtcag agggcaaaca gactccctgc atcgaagcag
     2341 atccggtgcg cgcggaccgt tataccgcgt gcggacagag ggtcaatgcg ggttgacgac
     2401 ccagtggctt gagaatagag tctttcgcct gtattagacc cagtctaagc gaggagagcg
     2461 tcgtgaccct aaaaggcagc aagacggaaa aaaacctcaa ggatgcattc gcaggcgagt
     2521 cccaggccaa ccggcgttac ctgtatttcg cggccaaagc tgacgtcgag ggcttcaacg
     2581 acgtgtccgc cgtattccgc tcgaccgcgg aaggggaaac cggacacgcg cacggacatc
     2641 tcgaatacct cgaggccgtc ggcgatcccg cgaccgggct gccgatcggc gaaaccaagt
     2701 tgaatctgaa agctgccatc gccggcgaga cccacgagta cacggacatg tatccgggca
     2761 tggcgaaagc ggcacgcgat gagggcttcg ccgagatcgc ggattggttc gagacactcg
     2821 ccaaggccga gcgctcccac gccaaccgct tccagaaggc tctcgacgcg atctagcggc
     2881 ccacgggctc gcactcctga tccacccggc cccgccgcag aagcgggacc gggtggtctc
     2941 acactttcgg cgcgcggaca gtcaacactc atgagctcag aaggggcccc atccgaaggt
     3001 cagtccggct ctggcaccgg cgggcgtgaa gggagtctgg aggcgcccac ccgacacccg
     3061 atcgattggc gcaatccgca gttctacgac gaggcggcgg tcactcgcga gctcgagcgc
     3121 gtgttcgaga tctgccacgg ctgccggcgc tgcttcagcc tctgcaactc gtttccaaca
     3181 ctgttcgatg cgatcgacgc ctcgaagacc atggagctcg atggtgtcga tcgcaaggtc
     3241 tactgggaag tggtggacaa ctgctacctg tgtgacatgt gcttcatgac caagtgtccc
     3301 tacgtgccgc cgcatccctg gaatgtcgac tttcctcacc tgatgctgcg cgcgaaggcg
     3361 actcatgcgc gccagaccgg cttcagcttt cgcgaccgga tgctctccag gaccgacacg
     3421 gtgggcaata ttgccggcct acccgtggtc gccgagctgg tgaatgcggt gaacggcacc
     3481 tcagccgggc gtgcgctgct gcagaaaacg ctcggcgtgg cgcgtgatgc accgctgccg
     3541 aagtattaca gccgtaccgc ccgcaaacgg ctgtccgatc gctccgatac ggacagcgcg
     3601 atcgccgatg ctgcggcggt gaaggcgacc gagcaaacga gcgggaaggt ggcgctcttc
     3661 gccacctgct acgccaatcg caacgagccc gagctcgccg aagatctcgc cgccgtgctg
     3721 gagcacaacg gcattgccgt gcggctcatt gcccaggagc gttgctgcgg catgccgcgg
     3781 ctggagctcg gggacctgga gggtgtggcg caactgaagg aattcaacat cccgcagctg
     3841 aagcgcgcag tggactcggg gttcgacctc atcgcgccga tcccttcctg cgtgctgatg
     3901 ttcaagcagg agctgccgct gatgtttccg caggacgccg atgtcagagc cgtgcagcgg
     3961 cgcatcttcg atccgttcga atatctcatg ttgcgccacc gtgccggcct gctgcgcacc
     4021 gacttcaagt cgccgctggg gaagatcagc taccacgtgc cgtgccacct gcgtgtgcag
     4081 aacatggggc tgaagacccg ggatgtgctg aaactggtgc cggagacgac catcgaggtc
     4141 atcgagcgct gctcggggca caacggcacg tatgccgtga agaaggagtt ccgcgccgct
     4201 tccgtgaaga tcggccggcc tgtcgtgcag cgcatcgaga gcgccaagcc cgatcattat
     4261 tgcagcgact gtccgatggc cgggcaccag ctcgaaagcg ggctcgcgca gccgcgcgag
     4321 ccggagcatc ccctgaagct gctgcgcgcg gcctatggca tctgaggctc gcatgaacaa
     4381 actctccagc ggcgacctgt tctccctcga gcgctatgcc cgcgagcgcg ccgccttccg
     4441 tgcccaggtg ctcgaacaca agcgccaccg gcagctgccg atcgggccga acgtgacctg
     4501 gctgttcgag gaccgcctca ccatccagta ccaggtgcag gagatgctcc gcgccgagcg
     4561 catcttcgag cccgagggga tcgaagagga gctcgagtcc tacaaccctc tcatccccga
     4621 tggcaccaac tggaaggtga ccctgctgat cgaattcccg gacgagcagg aacgccgcca
     4681 ggcgctggtg cgcctgaatg gcatcgagaa tcgctgctgg gtccgggtga gcggacacga
     4741 gacagtgttc gcggtggcag atgaggatct cgagcgcgcg aacgatgaga aaaccgcagc
     4801 ggtgcacttc ctgcgcttcg agctcagcgg gccgatgatc gcgagcctgc agggcggcgt
     4861 gggacttgcc gcggggatcg accacccgca ttaccggcac gtcgtggagc tcgtgagcgg
     4921 gcccgttcgg gatgcgttga ccgctgattt cgcttgagtt ttgaggccaa cggcctcatt
     4981 ttgggtcatt gccgccggca acgatccgct cccgccgtgc ccgacatttt gtaagattcc
     5041 gcgccgcacg cgcggcatcg ccgcccgcgg cgatcccggc ccctcctcag ccagacgacg
     5101 agcaccgcca cgagcatgag tcagtcctat accgcctccg acatcgaagt cctgagcgga
     5161 ctcgagccgg tccggcgccg gccaggcatg tacacgcata cctcgcgccc gaaccacctt
     5221 gcccatgaag tgatcgacaa cagtgtcgat gaggcgattg caggccactg caagcagatc
     5281 gatgtgacgc tctacaagga cggctcgctc gaagtggccg atgacggccg tggcatgccg
     5341 gtggacatcc atccgaagga gaaggtcagc ggtgtggagc tgatcctgac gcgcctgcat
     5401 gccggcggaa agttctccga tcagacctac aggttctcag gcggactgca cggggtgggc
     5461 gtgtcggtcg tcaatgcgct ctcgaagcac ctggagtgct ggatccggcg cggcggcaag
     5521 gaatacaaca tcagcttcaa ggacggaaag gtgagctcga agctcgaggt catcggcacc
     5581 gtgggccagc gcaacacggg cacgacgatc cgcttctggc ccgatccgca gttcttcgac
     5641 agcgataaat tctcggtagc accgctgaag cacaccctga aggcgaaggc agtgctctcc
     5701 ccaggtctgc gcgtgacctt taccaacgag gccaccaagg agaaggacga gtggttcttc
     5761 accggtgacc tcggtgcata cctgctggaa gagctgggct ccggagagaa gctccccgcc
     5821 gagccgatca tcggtccggg tgaggccggc aacgaggcgg tgagctatgc gctgctgtgg
     5881 gcgccgcagg ccgaagccgt ggtggccgag agttacgtca acctgattcc aacgatcgag
     5941 ggtggcacgc acgtcaacgg cctgcgctcg ggagttgccc aggcggtgcg cgagttctgt
     6001 gagtttcgca atctggtgcc gcgcggcatc aagctcaccc cggaggacat ctgggacaag
     6061 gcctgctatg tgctgtcggt gaagatccag gagccgcagt ttgcgggaca gatgaaagag
     6121 cgcctcggct cccgcgatgc ggcgcagctg gtggaaagcc aggtccgcga tgccgtggcc
     6181 ctctggctca atcggcatcc ggatgccggt gagcgcattg cgcagtttgc tatttccaat
     6241 gcccaggaaa ggctcaaagc cgcgcagcgc gtggtgcgca agcgctcctc cagcggtcct
     6301 gcattgccgg ggaagctcgc cgactgctcg agccaggagc ccaggcgctc ggagctgttc
     6361 ctggtggagg gcgattcggc gggcggctcg gcaaagcagg cccgcgagcg cgatttccag
     6421 gcgatcatgc ccttgcgcgg caagatcctg aacacctggg agctggagcc cggcgagatc
     6481 ggcgcctcgc aggaggtgca cgacatctcc gtcgccctcg gcgtcgagcc gggctcgacc
     6541 gacctctcgc agctccgcta tcacaaggtg tgtattctcg ccgacgccga ttccgacggt
     6601 cagcacatcg caaccctgct gtgtgcgctg ttcctgcggc atttccgtcc gctggtcgcc
     6661 aacgggcacg tgtatgtggc catgccgccg ctgtaccgga tcgatgccgg caaacaggtc
     6721 ctgtacgcac tcgatgacag cgagcgcgat cagctgctga agaaagtcga gaaggagaat
     6781 ccgcgtacgc ggccgagcgt cacgcgcttc aagggactgg gtgagatgaa tccggcgcag
     6841 ttgcgcgaga cgaccatcga cccgcgcacc cgacggctgg tgcagctcac gctcgaaggc
     6901 aaggacaaca ccgatcagct catggacatg ctgcttgcga agaagcgcgc ggcggaccgg
     6961 cgcgagtggc tggagcagaa cggcaacctg gcggaagtac tgtag
//
LOCUS       DASRHN010000279        14257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2401200, whole genome shotgun sequence.
ACCESSION   DASRHN010000279 DASRHN010000000
VERSION     DASRHN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401200"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1000)
                     /locus_tag="VFB37_11200"
     CDS             complement(188..1000)
                     /locus_tag="VFB37_11200"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HZO23058.1"
                     /translation="MKIGRQEQRASAISTANAETILVRGRDLARELIGKVNFTEHFWL
                     LVTGVLPSAAQCRVLDAALVAIAEHGLVPSVQAARMTLAAAPEAVQGAVAAGLLGCGS
                     VVLGASEAAGRFFAEVEARTSAGAAMHVAAAAVVGEYRANKRTIPGYGHPLHRGSDPR
                     AVRLLEVAREAGSAGDYVQIAATVERLLPELTGRALTLNISGAIPAVLLDAGYPLLAL
                     KGVPLLARTAGLVGHLLEEQQRAIGFVMSHAAAQAISYDGEAPPGFEPSQDA"
     gene            1133..1942
                     /locus_tag="VFB37_11205"
     CDS             1133..1942
                     /locus_tag="VFB37_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZO23059.1"
                     /translation="MVRRRTVPSRRESMGGLAKGLAVIRAFTREHAALTLSDIARSAQ
                     IPPATARRCLLTLEELGYVTRSGRSFLLRPKVLELGAAYLESMNIEQITKTHLEELAS
                     RTADSAALSVLDGTEIVYVARTSVRTLLRLEAHVGSRFPVHATSMGRVLLAGLSPERL
                     QQYLQTARFEALTECTVTDPERLRTLVAECRRCGYAVVQDELAYGVIALAVPVFDRRG
                     RVVASLNSSSHSRRTTKARLVRERLAMLQEVSRQISADLATTPWLALSAQL"
     gene            2039..2887
                     /locus_tag="VFB37_11210"
     CDS             2039..2887
                     /locus_tag="VFB37_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO23060.1"
                     /translation="MPAMTGRASALKATTAGDHYFTVAGARLRYRDEGRGAALVCVHG
                     WTLGLEMWDAQVAALRDCFRIVRFDRRGHGLSEGISSPERDAADLRALCEHLALRDVA
                     LLGMSQGARVAVHALHAGQRVWALILDGPPGLSADAETDIPAERYRSLALEQGMSAFR
                     REWKRHPLTQLRTAEPARRACLEAMLARYSGNDLWAPAAPSPTVQPAPLAVPTLILSG
                     QFDLPTRIQAADELASLLAAKRAVISGAGHLANLDCPDAYSKCCCDFLMGYVPDCVPV
                     SGVSHA"
     gene            2880..3506
                     /gene="wrbA"
                     /locus_tag="VFB37_11215"
     CDS             2880..3506
                     /gene="wrbA"
                     /locus_tag="VFB37_11215"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HZO23061.1"
                     /translation="MRKALVVYYSSYGHIEAMAYAQAEGVGRVAGVEVVVKRVPELTP
                     PEVARASGFKLEQKAPLAVPDELDQYDMILFGTPTRFGNMAAQMRNFLDQTGPLWVRG
                     ALVGKVGSVFCSTASQHGGQETTITSFHSTLLHHGMVIVGVPYTYKDLATMREVTGGT
                     PYGASCVTGAGNELRMPTALELDICRFQGEHVAKITSRLERETEGAHG"
     gene            complement(3547..4860)
                     /locus_tag="VFB37_11220"
     CDS             complement(3547..4860)
                     /locus_tag="VFB37_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZO23062.1"
                     /translation="MLQVGLPWGAALLVGLVSVLYASWSARASTAFLGWITGRPWLAF
                     LITPTLTVLALFLTRRWFTGSEGSGIPQVIAALHAPRPEEEHTLMRRLFGLRVIVGKI
                     CVSLIGLLGGLTIGREGPTVHIGAAIMAEIRRFYPHRSAQLERRLLLAGAAAGLSAAF
                     NTPLAGIIFAIEELARDFESRTNGIMITAVVFSGLTSLALAGNYLYFGQINVPENLGG
                     AFVLPVVLSAALCGLFGAAFNLALLRWRTWMPDWLIALRARRPLLYALAVGIVAAALG
                     VATHGETWGSGYDQARQLLTGTAPLSEGYAITKWGAMVVTYMAGIPGGLFSPSLSIGA
                     GLAQWVHAVFAWAPMAGVIALCMTGYLAAVTQSPITSFVIVMEMTNGTGMVIPMMATA
                     LVASRISAFFTPPLYEALAQSNYFSVATLPTGSFLQKKPMPPPGA"
     gene            5316..5690
                     /locus_tag="VFB37_11225"
     CDS             5316..5690
                     /locus_tag="VFB37_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23063.1"
                     /translation="MNDPFEENMFKALQSIPVAVLLLIATLLEVSGDAVVRLAIYRQS
                     GLLRMALILAAALLLLGYGSLLNTAPVAFARIVGLYIATLFVVWQIISFLFFRTLPGL
                     PVWLGGALVVAGGIIVTFWRPS"
     gene            complement(5761..6549)
                     /locus_tag="VFB37_11230"
     CDS             complement(5761..6549)
                     /locus_tag="VFB37_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZO23064.1"
                     /translation="MRYLTLLLLTLMAGCSSYAPDAGHEVVLVEKPWLFGHGGVNPDP
                     VKTGRTFTAVTTEGVDVEMRPQRYEEELPDTMTSDGVPVTFHAIVTLQVTDSVALIRN
                     FGSRWYQNNVEQAFGQFVRQAVRKHGMNETAISTIALDAIDAEIRDDLRKFIEDKKLP
                     VQLVTMTVGRANPPDAIKNQRIETAAQEQRVQTERQIKLAEDQRKAAEESRAAADNAY
                     RQALGLSPEQYVQLKRIEMERAVCESGKCTFIENGGAVPALAVR"
     gene            6692..7669
                     /locus_tag="VFB37_11235"
     CDS             6692..7669
                     /locus_tag="VFB37_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23065.1"
                     /translation="MFRSAGQINAVGGALAAGVLLVSLISSPRAQAEAVASSAELPEV
                     TVNAPRPPTPQELAGEAAPDFVRVHAKAAIVTGQLARWSIPICPMTQGLSPAFNDFVS
                     ARILAVAANVGAPHQEGDKCGTRHNVYIFFTSEPKRLLDEVVKKDSRLLGFHYPSQTR
                     SVQQMSRPIQGWYVTSSRGAWGDQSIDEAEPLLPSERSILNKGTHPAGMPDSRLSSHI
                     SSGIVNVLIVADLSRVVGLEIGPLSDYLAMLTLTQAFASEQCGTLPSIMDLMAPNCGD
                     REKPTQITAGDLAFLRALYQADLEAVLPMERSSISDSMLRAFKERPSTH"
     gene            complement(7663..8898)
                     /locus_tag="VFB37_11240"
     CDS             complement(7663..8898)
                     /locus_tag="VFB37_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO23066.1"
                     /translation="MSSNRHSSSDIADPGSQADPASPDGPPLGLHLRYAFEPGQQSGA
                     ELGNPLFDLLAAVLRTGSIRHAAQALGCSYRHLWGSLRKWEQILGEPLLLWSQGQRAR
                     PTPFAEKLLWAERRARTRMQPHIEALRSDLGRVLAEARDERHQLLSMRASHDIALPLL
                     QQHLAKNIDLHLDIRFQGSVDSLRALNDRQCIVAGFHVPALRATSPVFSKALKPLLKP
                     DIHRLIGCSRRMQGLMMRREHVGIIRTFPDIVRQGLRFVNRQRGSGTRMLVEHLLHEH
                     SIGPDWVRAYERHVEETHVAVAACVASGLADAGVGVEAAAIQFGLQFTPLVEENYFLA
                     CLRTNFAQPAVQRLCSALAGEGWSDILAGLPGYRAATAPGKLLVLTEVLPWWRESRAR
                     QAEHADARADRPESVPSPQ"
     gene            complement(8943..9737)
                     /locus_tag="VFB37_11245"
     CDS             complement(8943..9737)
                     /locus_tag="VFB37_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO23067.1"
                     /translation="MVLSEPLIEMRNAAFAYGARAVFSGLDLDVNQGELLSILGPNGC
                     GKSTLLRCLGGALSLNEGSVRLGKTELSSLPPNIRARRVGFLFQQHTPSFPFRVFDVV
                     SMGRAPYLGLFDVPSAADATLIAEALERVGMLHARERPYTELSGGERQLVLLARTLVQ
                     QPEVILLDEPTSHLDFRNQVVSLRMISALVQQGITMVMTTHDPNHALLFPGRVALMKP
                     GGTLLTGPAAEIITDATLTATYGIDIATFAVPRHSGSGDLRLCSPW"
     gene            complement(9728..10732)
                     /locus_tag="VFB37_11250"
     CDS             complement(9728..10732)
                     /locus_tag="VFB37_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZO23068.1"
                     /translation="MSKPRNLVLMLILPLGLLYVSLFVGRFPVAPQQVAHILYAQLVP
                     TAPDWPRSIETIVLQIRLPRALLAMLVGAGLSISGAAYQGMFRNPLVSTDILGVTAAS
                     GFGAAVALLLSFGAVKLQLVAFAFGLAGVALTYALARVYRTTPVLMLVLSGVVVAAFF
                     SALLSGAKYLADPESKLPAITYWLLGSLNSASMRSLAMAAPPIIVGSAGLLLARWKLN
                     ILAMGDEEARALGVRTEWLKGMVIVCTTLITAAAVSVCGIVGWVGLVIPHVGRMLVGP
                     DHRHLLPATLSIGATYLLFIDDIARSVSATEIPLGILTAVVGAPFFAYLLRRTGGTWY
                     "
     gene            complement(10729..11793)
                     /locus_tag="VFB37_11255"
     CDS             complement(10729..11793)
                     /locus_tag="VFB37_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO23069.1"
                     /translation="MNRLRRLLSAACLAAAACLAVAATAQPSDPRAAPTRQITDAAGR
                     VVTLPATVNRVADAWHANNSTVLMLGGASKLVATTVQARKQPWLQRLYPRIESLPAAF
                     NAAGEVNVETLIGARPDVILMAYDGSLPKWFDTISPYPIAVVLMPNTSLEDLATTARL
                     TGQVLGERESRVAADYVRYFEGNIHRVRQITDRLAPSERPKVLHTSSAGILTADGLDT
                     LIDDWIHVAGGVNAAQVKGNGRPVSLEQIIAWNPDVIIVGSAPNQQSRAAILADPRWS
                     GIKAVRTGKVYANPSGAYLWDRHGAESALQVLWAAKLLHPQDFADLDVRRETKLFYSR
                     FFHYTLTDAELRSIMNATAP"
     gene            complement(11806..14034)
                     /locus_tag="VFB37_11260"
     CDS             complement(11806..14034)
                     /locus_tag="VFB37_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZO23070.1"
                     /translation="MTGSTLQIALLATAFTWAAEIATAQDGALQQDAAQQPPAQQAAS
                     QQPTSAPQPALPHVLVTAERTPDEGDYRVKTVDSLGPLGTTPLQDIPYSVGVLPEQII
                     KNSQATNFKDVSKYLPLVSYQEQQGPDILRPQTRGMQGGNFQNTRLDGMQMFITVANA
                     MEQFQQIEVVNGPSSFLYGPANPSGMFNFVSKRPTGADLREVSVTYDSNSVGSAVMDL
                     SGALDQGGVVRYRFNGVFGEGEGFVDRSHVRRVLGDLGVDIRPFENTVLELNYSDYHL
                     LDHGFPGWFTYGEKIDLPPAPDPTRVGYGQSYAGVDLETRIGTARIKHDFSPDWHLVA
                     GVLNQDASRDINTPVNNVTSNSGNYTSSVANGFAPRFVMSSDVGYLNGNFATGSLTHD
                     LTIGTAGYKSVSYSVRTPATPASVLLGKASFTDPLIFAPPAGGLPNVGLNFDSSNAYQ
                     QGVNVGDLVHFNEQWAARLGVSQDWFHTTNFKASGAELPEYSSSGASPTASLIFKPLP
                     EVTTYATYASSLQAGDLAPTGTVNAGTSLAPYRSDEIEAGLKATVSSIDFTAAVYRIK
                     RPFANINLTDNVFEISGDQVNKGLELSAIGTVATGLTMYGGLTLLDPKLENTGVASTN
                     DKLFVGQPKIKGNVLLEYAIPAIQGLVTSFDYQFAGTRAGDDTNKFFVAGYNLFDLGV
                     RYSSPMVGKNVTWRLAVNNITDRHYWSTVAPSNLTGTNTGSLIGHLGLPRMVLASVSV
                     DF"
BASE COUNT         2590 a         4491 c         4601 g         2575 t
ORIGIN      
        1 tataggcctg gaagtgcggt gagtactgcc caccctggta ggcgcggtag ggatcccctt
       61 cggggctctc gactttgatc acatctgcac cgaggtcggc gagcatcatg ccggcgcacg
      121 ggccggtgat gaaggttcct tgctccacga cacggattcc ggcgagggcg gcgatcaagg
      181 cgtgctccta ggcatcctgg ctcggctcga agccgggcgg cgcctcgccg tcgtaactga
      241 ttgcctgcgc ggcggcgtgc gacatcacga agccgatcgc ccgctgttgc tcctcgagca
      301 agtgccccac gagccctgcg gtgcgcgcaa gcaggggcac tcccttgagt gcgagcagtg
      361 gataaccggc atcgagaagc accgccggga tcgctccgga aatgttgagt gtcagcgctc
      421 ggcctgtgag ctccggcaag aggcgctcga ccgtggcggc gatctgcacg tagtcgccgg
      481 cgctgcctgc ctcgcgggca acctccaaca ggcgcaccgc cctcggatcg gagccacgat
      541 gcagcggatg tccatacccg ggaatggtcc gcttgttcgc gcggtattca ccgacaaccg
      601 ccgcggccgc aacgtgcatc gcagcgccag cggaagtgcg agcctcgact tcggcaaaga
      661 agcgtcccgc tgcctccgag gcaccgagca cgaccgagcc gcagccgagc agccctgccg
      721 ccacggcgcc ctgcactgcc tccggggccg cggcgagtgt catgcgcgcg gcctgcacgc
      781 tcggcaccag cccgtgctcg gcgatcgcga cgagcgccgc atccagaacc cgacactgcg
      841 ctgccgaagg caggacaccg gtcacgagca gccagaagtg ctcggtgaaa ttgaccttac
      901 cgatgagctc gcgcgccaga tcgcggccgc gcaccaggat ggtctcggcg tttgcggtcg
      961 agatcgccga agcgcgctgt tcctgacgtc cgatcttcac gggccctctt tgccgctttc
     1021 gctatgcgaa attatcgtcg ttattcgaat agatactggc ctgccctcgg gccagcgtca
     1081 aggcaaggtg gggtgacttt cttgttttcg gatatcgaaa tagacttgca ccgtggtacg
     1141 acgcaggact gtccccagcc gccgcgagtc catggggggc ctcgccaagg gactggctgt
     1201 cattcgcgcc ttcacgcgcg aacacgccgc gctcactctg agcgacatcg cacgcagcgc
     1261 gcagattccc ccggcaacgg ctcgccgctg tctgctgaca ctcgaggagc tgggctacgt
     1321 cacccgcagc ggccgcagct ttcttctgcg gcccaaggtg ctcgagctgg gtgccgccta
     1381 cctcgagtcg atgaacatcg agcagatcac caagactcat ctcgaggagc tggccagcag
     1441 gactgcggac tcggcggcgc tcagtgtgct cgacggtacc gagatcgtgt acgtggcgcg
     1501 cacttcagtg cgtacgctgc ttcggctcga ggcacacgtg ggcagccgat ttccggtcca
     1561 cgccacttcg atgggccgcg tgctgctcgc gggcttgagt cccgagcggc tgcagcagta
     1621 cctgcagact gccaggttcg aagccctgac ggagtgcacg gtcaccgacc cggaacggct
     1681 gcgtaccctc gtggcagagt gccgccgctg cggctacgca gtagtgcagg acgagctggc
     1741 ctatggcgtg atcgcgctgg ccgtgccggt gttcgatcgc cgcggcaggg tcgtcgcctc
     1801 gctcaattcc tcgagccatt cgcggcgcac gaccaaagcg cgactggtac gtgaacggct
     1861 cgcgatgctg caggaggtca gccggcaaat ctccgccgac ctggccacca ccccctggct
     1921 cgcactcagc gcccagctct gaaacgcgca tccgagccct cgccttgacg caggagatca
     1981 ttgcgccgta gcctgtttgc gaaaacattt tcgctatccg aatgcagcag caaccaagat
     2041 gcctgccatg acgggccgcg cgtcagctct gaaagccact acggccgggg accattactt
     2101 cacggtcgcc ggtgcacggc tgcgctaccg tgatgaaggg cgcggggctg cgcttgtgtg
     2161 cgtgcacggc tggacactgg gacttgagat gtgggatgcg caggtcgcag ccctgcgcga
     2221 ttgctttcga attgtgcgtt tcgaccgtcg tggtcatgga ctctccgagg gcatctccag
     2281 ccccgagcgc gacgccgccg atctcagggc gctctgtgaa cacctggcgc tgcgggatgt
     2341 cgcgttgctc ggcatgtccc agggcgcacg cgtcgcagtt cacgcgcttc acgcaggcca
     2401 gcgcgtctgg gcgctcattc tcgacggacc tccgggtctg agtgccgatg cggaaaccga
     2461 catcccggcc gagcgttacc gcagtctggc actcgaacag ggcatgagtg ctttccgcag
     2521 ggaatggaag cggcatccat tgacccagct gcgtaccgct gaacctgcca ggcgcgcgtg
     2581 cctcgaagca atgctggcgc gttactcggg caatgacctg tgggcgcccg ccgccccgag
     2641 tccaacggtg cagcctgcac cgctcgcggt tcccaccttg attctgagcg ggcagtttga
     2701 cctgcccacg cgcatccagg cagccgatga gctcgccagc ctgctcgccg ccaagcgcgc
     2761 cgtcatttcc ggtgccgggc acctggccaa cctcgactgc cccgacgcgt acagcaaatg
     2821 ctgttgcgac tttctgatgg gatatgttcc cgattgtgtt ccggtctcag gggtatcaca
     2881 tgcgtaaggc acttgtcgtt tactacagca gttacggtca catcgaagcc atggcatatg
     2941 cgcaagcgga gggagtaggg cgcgtagccg gcgtcgaggt cgtggtgaaa cgagtgccgg
     3001 agctcacacc gcccgaggtg gcgcgcgcat cggggttcaa gctcgagcag aaggcgccgc
     3061 tggcggtgcc agatgagctc gaccaatacg acatgatcct cttcggtaca ccgacccgtt
     3121 ttggcaacat ggctgcacag atgcgcaact ttctcgatca gaccggaccg ctatgggtgc
     3181 gcggggccct ggttgggaaa gtgggcagcg tcttttgcag cacagcgagc caacacggtg
     3241 ggcaagaaac gacgatcacc tcattccata gcacgctctt gcaccacggc atggtcatcg
     3301 tcggtgtgcc ttacacgtac aaggatttgg caaccatgcg tgaggtgacg ggcggtacgc
     3361 cctatggcgc cagctgcgtg acgggagccg gcaatgaact gcgcatgccg acggcactcg
     3421 aactggatat ctgccgcttc cagggggagc acgtcgcgaa gatcacgagc cgcctggagc
     3481 gggaaactga gggcgcccat ggctagcggc gtgggcggta cgtgagtaaa ggcgcgttgg
     3541 gaccttttac gcgccgggag gcggcatcgg cttcttttgc agaaagctcc ctgtaggcag
     3601 ggtcgctacc gagaagtagt tgctctgggc gagtgcttcg tacaggggcg gcgtgaaaaa
     3661 ggcggagata cgggaggcaa ccagggcggt tgccatcatc ggtatcacca tccctgtacc
     3721 gttagtcatc tccatcacga tgacgaaaga tgtgatgggg gactgtgtga cagccgccag
     3781 gtatcccgtc atgcacagcg cgatcacccc cgccatgggg gcccaggcaa acaccgcatg
     3841 cacccactgc gcaagccccg caccgatcga cagggatggt gagaaaagac cacctggtat
     3901 ccccgccatg taagtcacca ccatggcgcc ccatttggtg atcgcatagc cttcggaaag
     3961 gggcgcggtg cccgtcaaga gctggcgtgc ctggtcgtag ccgctgcccc aggtctcgcc
     4021 gtgcgtggcg acaccgagtg ccgccgccac gatcccgact gccagggcat acagcaacgg
     4081 cctgcgcgcg cgtagcgcga tgagccagtc tggcatccac gttcgccagc gcagcagggc
     4141 caggttgaat gcggcaccga atagcccaca gagcgccgcc gacaacacca ccggcaggac
     4201 gaacgctccg cccaggttct ccggtacgtt gatctgcccg aagtacagat agttgcccgc
     4261 caacgcgagc gaagtcaggc cggagaacac cacggccgtg atcatgattc cgttggtacg
     4321 gctctcgaaa tcccgtgcca gctcctcgat agcgaagatg atacctgcga gtggcgtatt
     4381 gaaggctgcc gagagtcccg ccgccgcacc ggcgagcagg agccggcgtt cgagttgcgc
     4441 gctgcggtgc ggatagaagc gccggatttc cgccatgatc gccgcgccaa tgtgtaccgt
     4501 cggcccctcg cgccctatcg tgaggccgcc cagcaggccg atcagcgaaa cgcagatctt
     4561 tccaacgatc acgcgtagac cgaagaggcg tcgcatcagc gtgtgttctt cttccgggcg
     4621 cggtgcgtgc agcgccgcaa tcacctgcgg aatgccgctg ccctcgctgc ccgtaaacca
     4681 gcgcctggtg agaaacagtg ccaggacggt cagcgtcggt gtgatgagga aggcgagcca
     4741 agggcggccg gtaatccagc ccaggaaagc ggtggaagcg cgggcgctcc aggaagcata
     4801 caacaccgag acgaggccca cgagtagcgc tgcgccccac ggcagcccta cctgcagcaa
     4861 gagccgtctg gattgagcac cgagcacccg gcgcttcgcg tgttcgcgga gcttccgccg
     4921 tgagtgttcg cctggacctg cctcggagtc tttcgctggg tcgcccccct gttcatgccg
     4981 cgccgaaggc ctctcggcga cgtcagtgcg cagcttcagg cgctgccgcc agttcatcga
     5041 cgcccgaaat cacggccgag agtctgcgaa gctcaccact tcccgccgtt cgagcttcat
     5101 acctgcaccc actcacatgc tagcattttt gtgtgcggtc tcgcgtgaca gccaggaagc
     5161 catggcggct gcatcgggcg caggcagtac gcttgtgaag cgtcgcgacc cggaccagtg
     5221 cagcgaagtc tggctcactg gcagcacgag gcacgagctt agccggtacc gacattcccg
     5281 cagccacacc acctgtttga taagcttgcc tcgcagtgaa cgatcctttc gaggagaaca
     5341 tgttcaaggc cttgcagagt attcccgttg cagtgttgct tctgattgcg accctgcttg
     5401 aggttagtgg agatgccgtc gtgcggctcg caatctacag gcagtctgga ctgttgcgca
     5461 tggcgctcat cctggcggcc gcgctgcttc tcttgggata tgggtcgttg ctaaacaccg
     5521 cgccagtggc cttcgcgcgc atagtcggcc tctatattgc gacgctgttt gtggtctggc
     5581 agatcatcag cttcctattc ttccggacac ttccgggatt acctgtctgg ttgggtggtg
     5641 cgctcgtggt tgcgggcggc atcatcgtta ccttctggcg tcccagctga aatttccggg
     5701 cggcgcggcg cgttctaatt caacagttca cgactccttc gagcgttgca cgctcgatca
     5761 tcaccggact gcgagtgcag gaacggcgcc gccattctca atgaacgtgc acttgccgct
     5821 ctcgcagacc gcccgctcca tctcgatccg cttgagctgc acgtactgct cgggcgagag
     5881 cccgagggcc tgccggtagg cgttgtcggc tgcagcacgg gattcttccg ccgccttgcg
     5941 ctgatcctcg gcaagcttga tctgccgctc ggtctgcacg cgctgctcct gcgcggcggt
     6001 ctcaatcctc tggttcttga tggcatccgg gggattcgcc cgtccgacgg tcattgtgac
     6061 cagctgcacg ggcagtttct tatcctcgat gaacttccgc aggtcgtcac ggatctcggc
     6121 gtcgatcgcg tccagggcga tcgtggagat ggccgtctcg ttcatgccgt gcttgcgaac
     6181 agcttggcgg acaaactggc cgaaggcctg ttcgacgttg ttttgatacc aacgtgagcc
     6241 gaagttgcga atcagcgcca cggagtcagt gacctgcaga gtcacgatgg cgtggaatgt
     6301 cacggggacc ccgtccgaag tcatcgtatc cggcaattct tcctcgtagc gctggggacg
     6361 catttccaca tcgacgccct cagtggtcac cgccgtgaag gtacgcccgg tcttcactgg
     6421 atcggggttc acgccgccat gcccgaagag ccagggtttc tcaacgagaa ccacttcatg
     6481 gccagcatcc ggcgcgtacg aactgcagcc tgccatcagt gtgagaagca acagtgtcag
     6541 gtatctcatt attctcccct cgaaattaac tctggtgtcc cgggcgcggc agcggagtgt
     6601 agcagtgcta ctgcgctgct accgctctga gccgaacccg cccgaagcag caggatggtg
     6661 ctaggctgag ttcctggaga aggcgcgcca agtgtttcgg tcggcagggc agatcaacgc
     6721 agtgggcggc gcgctcgcgg caggcgtgct gcttgtgtcc ctgatttcga gtccccgcgc
     6781 gcaggctgag gccgtggcga gttccgcgga gttgccggag gtcacggtaa acgcgcccag
     6841 gccgccgacg cctcaggagc tcgccggcga agccgctccg gactttgttc gcgtccacgc
     6901 caaagctgcg atcgtcaccg gacagctggc gcgctggagc atcccgatct gcccgatgac
     6961 ccagggactg tcacctgcat tcaacgattt cgtctcggca cgtattctcg cggttgccgc
     7021 caacgtcggt gcgccccatc aggaaggaga caaatgcggc acccggcaca acgtctacat
     7081 ttttttcacc agcgagccga agcgattact ggatgaggtg gtgaagaagg acagccggct
     7141 cctgggcttc cactatcctt cgcaaacccg gtctgtgcag cagatgagcc gccccattca
     7201 gggttggtat gtgacctctt cccgcggtgc ctggggagat caatcgatcg acgaagccga
     7261 gccgctgctg ccctcggaac gcagtatctt gaacaaaggg acgcatcctg cgggtatgcc
     7321 ggacagccgc ctgagcagtc acatcagcag cggaatcgtc aatgtgctga tcgttgccga
     7381 cttgagccgg gtcgtgggcc ttgagatcgg ccccctctcc gactatctag ccatgctgac
     7441 cttgacgcag gcgttcgctt ccgagcagtg tgggaccttg ccgagcatca tggatctgat
     7501 ggcgccgaac tgtggcgaca gggaaaagcc tacccagatt accgccggcg atctggcctt
     7561 cctgagagct ctgtaccagg ccgatctcga ggccgtgctg ccaatggagc gaagctccat
     7621 ttccgacagc atgctgcggg cgttcaaaga acggccgtcg actcactgag ggctgggaac
     7681 gctctctggc ctgtcagctc gagcgtccgc atgctcggct tgccgcgctc tcgactcccg
     7741 ccaccacggc aggacttcgg tcagcacgag cagcttgccg ggagcggtgg cagcccgata
     7801 gccgggcagg cctgccagaa tgtcgctcca gccttcgccg gccagtgcgc tgcagaggcg
     7861 ctgcacggcg ggctgcgcga agttggttcg caggcaggcg agaaaatagt tttcctccac
     7921 cagcggggtg aactgcagac cgaactggat cgcagccgcc tcgacaccca caccggcatc
     7981 ggccagtcca cttgccacgc aggcagcgac tgcgacatgg gtttcctcga cgtggcgctc
     8041 gtacgcccgc acccagtctg gtccgatcga atgctcgtgc agcagatgct cgacgagcat
     8101 gcgggtcccg gagccgcgct ggcggttcac gaagcgcagg ccctgccgga cgatatcggg
     8161 gaaggtgcga atgatgccca cgtgctcgcg gcgcatcatc agtccctgca tccgccgcga
     8221 gcagccgatc agacgatgga tgtccggctt gagcagcggt ttgagcgctt tggagaagac
     8281 cggtgaggtg gcgcgcagcg ccgggacgtg aaagccggcc acgatgcact gtcgatcgtt
     8341 cagcgcgcgg aggctgtcaa cgctgccctg aaagcggatg tccagatgca ggtcgatgtt
     8401 cttggccaga tgctgctgca gcaggggcag ggcgatgtcg tgactggccc gcatgctgag
     8461 cagttgatgg cgctcgtcac gggcctcggc cagtactcgc ccgagatccg agcgcaaggc
     8521 ctcgatgtgc ggctgcatgc gggtccgggc acggcgctcg gcccagagaa gcttctcggc
     8581 gaacggcgtc ggtctcgccc gctgaccctg cgaccacagc agcagcggct cgcccaggat
     8641 ctgctcccat ttgcgcaggg acccccacaa gtgacgatac gagcaaccga gggcctgcgc
     8701 ggcatgacgg atcgagccgg tacgcagcac ggcggcgagc aggtcgaaga ggggattccc
     8761 gagctcggcc ccactttgct ggccaggctc gaaggcgtat ctcaggtgca gcccgagggg
     8821 cggaccatcg ggacttgctg gatcagcctg gctccctggg tctgcgatat cggacgaaga
     8881 gtgacggttt gagctcacgg gagcgatcct agcaccgcgc gtccccggcg gcgcccggag
     8941 cactaccagg ggctgcagag cctcagatcg ccgcttccgg agtgacgcgg caccgcaaag
     9001 gtagcgatgt cgatcccata ggtggccgtg agcgtcgcat ccgtgatgat ctcggccgcc
     9061 ggtcccgtga gcagcgtgcc tcccggcttc atcagcgcca cccggccggg aaagagcaga
     9121 gcgtgattgg ggtcatgtgt cgtcatgacc atcgtgatgc cttgctgaac cagagcgctg
     9181 atcatccgca aggacaccac ctgatttctg aagtccagat gagaggtggg ctcatcgagc
     9241 aggatgacct ccggctgctg gacgagcgtc cgggcaagca ggacgagctg ccgctctccg
     9301 ccgctcaatt ccgtgtaggg gcgctcccgc gcgtgcaaca tgcccacccg ctcgagcgcc
     9361 tcagcaatca gtgtcgcatc cgctgccgag ggcacatcga acagccccag gtaaggtgcc
     9421 ctgcccatac tcaccacgtc gaaaacgcgg aacgggaatg acggggtgtg ctgttgaaaa
     9481 agaaagccta cgcggcgggc ccggatgttg ggtgggaggc tcgagagctc cgttttgccg
     9541 agccgcacgc ttccttcatt cagagagagt gcaccgccga ggcagcgcag cagcgtgctc
     9601 ttgccacagc cgttgggccc gagaatcgac aggagctcac cctgattcac gtccaggtcg
     9661 aggccggaaa ataccgctcg tgcaccgtag gcgaatgccg cattgcgcat ctcgatcaac
     9721 ggctcgctca gtaccatgtg ccccctgttc gacgcagcag ataggcgaag aacggtgcgc
     9781 cgaccacagc cgtcagaata ccgagaggga tctcagtggc gctcacgctc cgggcgatat
     9841 cgtcgatgaa cagcagataa gtggcaccga tgctgagggt ggcggggagc aggtgtcggt
     9901 gatccggtcc caccagcatg cgaccgacgt gcggaatgac caggcccacc cagccgacga
     9961 tgccgcacac cgaaacggct gcggcggtga tcaacgtggt acacacgatc accataccct
    10021 tcagccactc ggtgcgtacc cccagggcgc gagcttcctc atcgcccatc gcgagaatgt
    10081 tgagtttcca gcgcgcgagg agcagtccgg cgctgcccac gatgatcggc ggtgcggcca
    10141 tggcgaggct gcgcatggac gcggagttga ggctgccgag caaccagtat gtgattgcag
    10201 gcaacttgga ttccggatcg gcgagatatt tcgccccact cagcagggca ctgaagaacg
    10261 ctgcgacgac cacgcccgac agcacgagca tcaggaccgg ggtcgtccgg tacacgcgcg
    10321 cgagagcgta ggtcagcgcg acgcccgcca gtccgaaggc gaaagccacg agctgcaatt
    10381 tcactgcacc gaacgagaga agcagcgcga ccgctgcccc gaatccggag gcagccgtca
    10441 cgccgaggat gtcggtgctg acgagcggat tgcggaacat gccctggtag gctgcgccac
    10501 tgatcgagag ccccgcgccc accagcatcg cgagcagcgc cctcgggagc ctgatctgca
    10561 gcacgatcgt ctcgatcgac ctcggccagt caggagctgt ggggacgagc tgcgcgtaca
    10621 gaatatgggc cacctgttgc ggtgcgaccg gaaagcgccc cacgaacagc gagacataca
    10681 gcagccccag gggcaggatc agcatgagta cgagattgcg cggcttgctc aaggcgcggt
    10741 cgcattcatg atcgagcgca gctccgcatc cgtcagggtg taatggaaga accgggaata
    10801 gaataatttc gtttcccgcc tcacgtcgag atcggcgaaa tcctgcgggt gcagcagctt
    10861 tgcggcccac aggacctgca gcgcggattc cgcgccatgt cggtcccata aataggcccc
    10921 gcttggattc gcgtacacct tgccggttct caccgccttg atcccgctcc agcgggggtc
    10981 ggcgagtatg gcggcgcgac tctgctggtt gggcgcgctg ccgacgatga tcacatccgg
    11041 attccaggcg atgatctgtt ccaggctcac cggacggccg ttgcccttga cctgcgccgc
    11101 attcacgccc cccgcgacat gaatccagtc atcgatcaag gtgtcgaggc cgtcggcggt
    11161 caggatgccc gcggaggagg tgtgcagcac tttcggccgc tcggatggcg ccagacggtc
    11221 ggtgatctgc ctgactcgat ggatattgcc ctcgaaatag cgcacgtaat ctgcggcgac
    11281 gcgcgattcg cgctcgccga gcacctgccc cgtcagccgc gcggtggtcg cgaggtcctc
    11341 tagtgaggtg ttgggcatca gcacgactgc tatgggatag ggggagatcg tgtcgaacca
    11401 cttcgggagg gagccatcgt acgccatgag tatcacgtcg ggccgcgcgc cgatcagcgt
    11461 ctcaacgttg acctcgcccg ccgcattgaa agctgccggc aaggactcga tgcgcggata
    11521 caggcgctgc agccacggct gcttgcgtgc ctggacggtg gtcgcaacca gcttgctggc
    11581 gcccccgagc atcagcacgg tggaattgtt cgcatgccag gcatcggcca cgcgattcac
    11641 cgtggccggc agggtcacca ctcgaccggc cgcatccgtg atctggcggg tcggcgctgc
    11701 tctggggtca gacggctgcg cggttgccgc aacagcgagg caggctgcgg cagcgaggca
    11761 ggctgcgctg agcaatcgcc gcagccgatt catgggccag cagactcaga agtcgacgga
    11821 gacggaagcc agcaccatcc gcggcaggcc gagatggccg atcaggctgc cggtgttggt
    11881 gccggtcagg ttgctgggcg caacggtgga ccaataatgc cggtccgtga tgttgttgac
    11941 tgcgagtcgc caggtcacgt tcttacccac catcggcgag gagtagcgga ctccgagatc
    12001 gaagagattg taaccggcga cgaagaactt gttcgtatca tcacccgcgc gcgtccccgc
    12061 gaattgatag tcgaagctcg tcaccagtcc ctgaatcgcc ggaatggcat attcgagcaa
    12121 cacgttgccc ttgatcttgg gttgcccgac gaacagcttg tcgttcgtgc tggcgacccc
    12181 ggtattctcc agcttcggat ccagcagcgt cagacccccg tacatcgtga gccccgtggc
    12241 cacggtaccg atcgcggaaa gctccagacc cttgttcacc tggtcgccgg agatttcgaa
    12301 gacattgtcg gtcaggttga tgttggcgaa aggccgcttg atgcgataca ccgcggcggt
    12361 gaagtcgatg ctcgacaccg tcgccttgag gcctgcctcg atctcgtcgc tgcgataggg
    12421 tgcgaggctc gtgccggcat tcacggtacc cgtcggcgcc agatcgcccg cctgcaggct
    12481 gctcgcatag gtcgcatagg tcgtgacttc ggggagtggc ttgaagatca ggctcgcggt
    12541 gggactcgcg ccgctgctgg agtactcggg cagctctgcg ccgctggctt tgaaattcgt
    12601 ggtatggaac cagtcctggc tcaccccgag gcgggccgcc cactgctcat tgaagtggac
    12661 cagatcgccg acgttgaccc cctgctgata ggcgttcgag gagtcgaagt tgaggccgac
    12721 attcggcagg ccgcccgcgg gcggcgcgaa gatgagtgga tcggtgaagc tggccttccc
    12781 gagcagcacg ctcgccggcg tggcgggcgt ccggactgaa taagacactg acttgtaacc
    12841 ggccgtaccg atcgtcagat cgtgcgtcag ggacccggtt gcaaaatttc cattcaggta
    12901 gcccacgtcg ctcgacatca cgaagcgcgg cgcaaagcca ttggcgaccg atgaggtgta
    12961 gttccccgaa ttggacgtca cattattgac cggcgtattg atgtcccggg aggcatcctg
    13021 gttgaggacg ccggcgacga gatgccagtc ggggctgaag tcatgcttga tgcgcgccgt
    13081 gccgatgcga gtctcgagat cgacccccgc gtagctttgt ccgtaaccga cgcgtgtcgg
    13141 atccggagcg ggcgggagat cgatcttctc accgtaggtg aaccatccgg gaaagccgtg
    13201 atcgagcagg tgatagtcgc tgtagttgag ctcgaggacg gtgttctcga acggccggat
    13261 atcgacgccc agatccccga gcacccgccg gacatgactt cggtcgacga agccttctcc
    13321 ttcaccgaag acaccattga agcggtagcg gaccacgccg ccttgatcca gagcgccact
    13381 cagatccatc accgccgacc cgacgctgtt gctgtcgtag gtgacgctga cttctcgtaa
    13441 atcggccccc gtcgggcgct tggagacaaa gttgaacatg cctgaggggt tcgccggacc
    13501 gtagaggaac gaggagggcc cgttcacgac ctcgatctgc tggaactgct ccatggcatt
    13561 ggcgacggtg atgaacatct gcatcccgtc gagccgggtg ttctggaaat ttccaccctg
    13621 catgccgcgc gtttgcggac gcaggatgtc cggtccctgc tgctcctggt aggacaccaa
    13681 cggaaggtac ttcgagacat ccttgaagtt cgttgcttga gagttcttga tgatctgctc
    13741 gggcaacacg ccgacagagt aggggatgtc ctgcaggggc gtggtgccga gcgggcccag
    13801 cgaatccacc gttttaaccc ggtagtcgcc ctcatccggg gtgcgctcgg cagtgaccag
    13861 cacgtgtggc agggcaggct gcggagcgga ggtcggctgc tgagatgcgg cttgctgggc
    13921 gggtggttgc tgggcggcgt cctgctggag ggcgccatcc tgcgcggtgg cgatttctgc
    13981 tgcccaggtg aaggcggtcg cgaggagcgc gatctgcaaa gtggagccgg tcattggatt
    14041 tttgtcttcc ggtgaatggc cgggttcgct gtgacgcgcc atcgaagcgc gggtctcgct
    14101 cggccagaag ccagagaacc tagcacgcgc actccgcacg accaatatga acggccgcgc
    14161 atatgttggt ctgcgtagcg ttgcgagggg tcatattgtt gggattggtc gcagcacgtg
    14221 aattacgacc cggtggcagc acgcatgttg gcccacg
//
LOCUS       DASRHN010000280        16609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2237737, whole genome shotgun sequence.
ACCESSION   DASRHN010000280 DASRHN010000000
VERSION     DASRHN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237737"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="VFB37_11265"
     CDS             complement(<1..1672)
                     /locus_tag="VFB37_11265"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZO23071.1"
                     /translation="MRLLPLLIPLSLMPCALLAQTVPEPVPADEVFSPEQIGHALAAP
                     TPDAGKAAASRANAPEDDGQWTMPAKNFASTRFSSLTELTPENVKNLQPLLTFSLDVN
                     KGQEAAPIVADNTMYVVTAYPNYVYALDLTKPGAPLKWKFAPKPVPASQGVACCDVVN
                     RGGSVDDGKYIFTTLDGQVIALDVKTGKAVWRTRLGNINTGESITMAPLVAKGHVYVG
                     NSGGEMGVRGWLTALDESSGKLLWKAYSTGPDAEVLIGSEFKPAYASDRGTDLGVKSW
                     PPQAWKIGGGSVWGWPNYDAEFDTIYYGTGNPGPWNAEQRPGDNKWTAGIFARDPANG
                     SARWFYQFTPHDEHDYDGINEQILLDMPFGGQMRKVLIHFDRNGYIYVIDRKSGQVLS
                     ADPFSAVNSSRGVDLKTGRLIINPAKQTKLGEQVTDICPTASGAKDWQPSSFSPQTGL
                     IYIPHENICMDWMDMQVSYIEGTPYVGAQVHMKPGPGGNRGAVTAWDPVRRHAVWQAK
                     ENFPVWSGTVATGGGLVFYGTMDGWFKAVNARTGELVWRYKVDSGVIGQ"
     gene            complement(1690..2136)
                     /locus_tag="VFB37_11270"
     CDS             complement(1690..2136)
                     /locus_tag="VFB37_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23072.1"
                     /translation="MIAPETALRAPVTPGPSGASLTLSLFGLLGGPLAWFAQCCAGYG
                     FSSWACFPKDQRRFAPLEGYGWTWGVVVGISAVAFLIAVGAFLASSHIRQRIDGQSGQ
                     VAQLGLPIGLCRTRFLAVWGMAAGGGFAIAIAFTAVALIVLPQCAG"
     gene            complement(2133..2798)
                     /locus_tag="VFB37_11275"
     CDS             complement(2133..2798)
                     /locus_tag="VFB37_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZO23073.1"
                     /translation="MNGAGLAPSELEMRRAQRDRPVLLAEPGKLPVGPVGRRGLGLWG
                     VGTLIATEAALFAYLLFAYYYTGAVSPRGWLLESHPTLKLALPNTIVLLVSSVFAWYG
                     ETGVLRRQRSRALIGFSIALVLGIVFVLVQGFEWYARPFRLSDSSYASLYYITTGFHI
                     AHVIVGVLVLAAILLWTALDYFSPRRQLSVSAGVIYWHFVDLVWLLIFMTYYITPYLG
                     FGR"
     gene            complement(2795..4735)
                     /gene="ctaD"
                     /locus_tag="VFB37_11280"
     CDS             complement(2795..4735)
                     /gene="ctaD"
                     /locus_tag="VFB37_11280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZO23074.1"
                     /translation="MRVADFRAPAVGPTTAEHESRLKRLWETSPGLEGFLGTVDHKQI
                     GIRYIVTAFIFLALGGIEALVMRLQLAGPNLSLLTPEQYDQLFSTHGMTMIFLYASPI
                     LSGFSNYLWPLLLGSRDMAFPRLNALSYWIYLASGIFLYVGFLTGNGPNDGWFNYVPY
                     ADRDYNPGLNEDFYSLGMVFLGISTTIGAANFIVTAMRTRAPGMALNRMPVLIWGTLT
                     ASAANLVVVPAVSLAFFLLWLERQFGAHFFDPAGGGQPLLWQHLFWIFGHPWVYAIVL
                     PAMGMVSHALPVFCRRPLVGYGIVALSTVATMVLGFGVWVHHMFATGLPGLGLSFFSG
                     ASIAIVFPSAVAVFAWLATLWLGRPVITTAFLYFSSMILMFVIGGVSGFMTASVPVDW
                     QLTDTYFVVAHIHYVLIGINVFPVLGAVYFWFPKMTGRLMDERLGRWNFWLTFIGFNL
                     AFLPMHLTGLLGMPRRVYTYQDGMGWDSLNMITTIGSFVLALGVLLFIVNVLKSLRGG
                     ALAGPNPWDAYSLEWSVSSPPPPYNFAVIPTVASRYPLWEGRLRESGAVSCVDQGMLL
                     ESHSKEAIGTSSMDATPDMILEMPEDSTAPFVLTLGTALLFVGLLLKLWSIVIIGTVV
                     LAIATLFWLWPRRELREREVNP"
     gene            complement(4732..5697)
                     /gene="coxB"
                     /locus_tag="VFB37_11285"
     CDS             complement(4732..5697)
                     /gene="coxB"
                     /locus_tag="VFB37_11285"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZO23075.1"
                     /translation="MSGATPLTYLTAAGHRAATILPLTWFTLIISVLVCVIIAVLLWG
                     AIRRARGSGGAAETRSVEVEPGPSGARWISIGLLISAVPLLATLIWTMVAIAATSGPP
                     SPPGLTLDVTGHQWWWEVRYDAAEPDRSFETANEIHIPVGVPVRVRLHGGDVIHSFWV
                     PALTGKTDTIPGQTNLSWMEARGPGRFRGQCTEYCGAQHAHMGLEVVAQPQADFDRWR
                     AAQLRPAGAPQTSAQQRGLAVVEYRCGLCHQVRGTQAGAVAAPDLTHLMSRRTLAAGL
                     LPNTPGNLAGWIQNAQEFKPGTLMPNQYLSGQQLSDVQAYLESLQ"
     gene            complement(5694..6455)
                     /locus_tag="VFB37_11290"
     CDS             complement(5694..6455)
                     /locus_tag="VFB37_11290"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZO23076.1"
                     /translation="MPHETAAMEVLPYCGSPPAPGELLTRFNIDPVLILALVVLAGTH
                     LWAARGTHTRWYAAGGWLITAAALLSPLCALSVSLFSARVAQHMILVLLAAPLIALAW
                     PRASHRTGALGLWGSAAAFFLALWFWHMPVPYDLTFSSTPIYWTMHLTLFGSAVLLWR
                     ELLHHRSDRTADVLAAGALTSMQMGLLGAVLTFAGHPMFLWHLTTTAAWGFTPLHDQQ
                     LGGVFMWVPGIALFLCAALRSLGRLWRVLEEAGSP"
     gene            6788..7063
                     /locus_tag="VFB37_11295"
     CDS             6788..7063
                     /locus_tag="VFB37_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23077.1"
                     /translation="MNFEDALNIGLHAYRVERVEHGDAVEYRATLPDYPQCTGCGGTA
                     EEAVRTARHQLTQLRDSQAAVAVCDLEDERSDRLREAIRAVVTRALI"
     gene            complement(7092..8555)
                     /gene="zwf"
                     /locus_tag="VFB37_11300"
     CDS             complement(7092..8555)
                     /gene="zwf"
                     /locus_tag="VFB37_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZO23078.1"
                     /translation="MAKVLPGVGPRSAQVLVLVGATGDLARRKLLPGLFYLATAGFIP
                     ACRIVGVSLDSLDADGFRSVARAALNEFSTRKILEADWDVFARCLDYVPLSAGAAALA
                     GAVRKAEQAFSGDCERLHYLSVPPGAALSAVRLLGEAELVPRSRIVMEKPFGTDLASA
                     VSLNAKLHEVFAEEQIFRIDHFLGKEPAQNILAFRFANGLFEPIWNRNFIDHVQIDVP
                     ETLSLGTRAAFYEATGAYRDMVVTHLFQILAFMAMEPPTALEPAPISEEKNKVFRSML
                     PIQPTDVVRGQYTGYRSEPGVSADSETETFIALKCSIDNWRWAGVPFYLRTGKRLAEG
                     QRIISIAFREPPKSMFPPGSGVGAQGPDHLTFDLADASKMSLSFYGKRPGPGMRLDKL
                     SLQFAMHDTGLVGDVLEAYERLILDAMRGDHTLFTTAEGIERLWEVSMPLLEAPPPVR
                     HYAPGSWGPNAIHQLVAPSAWRLPFERAWRDPNRTGS"
     gene            complement(8633..10600)
                     /locus_tag="VFB37_11305"
     CDS             complement(8633..10600)
                     /locus_tag="VFB37_11305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZO23079.1"
                     /translation="MAERPATKLAYRPDIDGLRAVSVLAVIGYHAFPAVFPGGFFGVD
                     VFFVISGFLISSLIFGQLQRSTFSLGDFYRRRIRRILPALIIVLAACLAFGWYALLPE
                     EYGYLGRQVAAAAAFVSNFALWTETGYFAPATQLKPLLHLWSLGIEEQFYLLWPLLLL
                     LLSRRRLATGWVIALFTGGSFAWDLLLVRHHPTAAFYFPLSRFWELGLGCFMAWLSCG
                     SPGRPPPMRAWGAAVASLLSVAGLSMILVAVFCCNSHSGVPGWPSLLPTLGALCIVAQ
                     PGKSGFQRRILAYPPLVFVGLISYPLYLWHWPILSFAAILHSGTPNLLTRWACVVLGF
                     GLAILTYRLIEKPIRAGGHWRPLSPALLGSLVVAGLAGLTVFHAAGFGHRFDRQVAAI
                     RPQAKTDPLCEETVDTHIPFNYCKRTSEQPPDVVFLGDSQTEAVYEGAAAALGKSYSL
                     MMLARGGCPPTLNASVQGLYPSESAQQNCNKTWNEFVEYVQKARPRLVVLVGAGTRFF
                     DANSPKDASAKRAAFKRGMENLLGALQATSRVVYVREIPEFQSSPSCYLRPFRLPGTR
                     CSAFLARGALELHRSAYSTALEEVKDRFPSLVLVDPIPALCNSRYCSEQLQSGEMLYR
                     DEIHLTQAGGRRVAKGTGLLQLIRDTIDYPG"
     gene            10720..12027
                     /locus_tag="VFB37_11310"
     CDS             10720..12027
                     /locus_tag="VFB37_11310"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZO23080.1"
                     /translation="MIPQSRSFLPGLCLALLAALAPCLGFAADTDYHARLARILQRTP
                     LIDGHNDLPWEIRTRMHGQLAGIDLKSDTSKLRAPTGEAPLMTDIPRLRAGMVGGQFW
                     SVYVPADYKGFEAVQATVEQIDLVKRMAARYPADFEMAYTAADVRRIHKAGKIASLIG
                     IEGGHQIDNSLAVLRQMYELGARYMTLTHVLTTGWADSATDAPQHHGLTPFGQEVVRE
                     MNRLGMLVDLSHVSPDTMRAALQVTQAPVIFSHSSARALVDHPRDVPDDILKLVAANG
                     GVVMVNFAPEYVSEARNQWEADRAAEQARYSSPPYGGLYIGQPDRAKAALEKWDSAHP
                     RPVTTLAQVADHIDHVRQVAGVDCVGLGSDFDGIPDTPKGLEGVDHYPALLEELMRRG
                     WSDADVAKVAGENALRVLAGAERVAAKLRSERGPSEVLFEPGH"
     gene            complement(12030..13727)
                     /gene="ggt"
                     /locus_tag="VFB37_11315"
     CDS             complement(12030..13727)
                     /gene="ggt"
                     /locus_tag="VFB37_11315"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZO23081.1"
                     /translation="MTAAREFTAARGDRAGGWLGQTRSEVLARNGIVATSQPLAAQAG
                     LQILKQGGNAFDAAVATAAVLNVVEPAGAGLGGDVFVLAWVAREKQLVALNGSGRAAA
                     GATPERLAERGHTKEMPRRSIDAVTVPGAVDAWDVLLKRAGTLSFRETLEPAATLAEE
                     GFGLTERIYEDWVRDVETLRADRDSAAVYLIDGNAPRLYSTFRNPDLARALRVLQSRG
                     KEAFYQGEIARAIVAKSQALGGMLTLEDLAGTRATWEAPLSTHYHGFDVFEMPPNTQG
                     FAALEILNILEAYAEKSGVTLAELGPRSSAYWHLLVEAKKLAYADLYEFNADPAFTNV
                     PIEHLLSRSHAQELCAAIDPRAARTPEPRGDPVGGTVYIAAADRWGNMVSFIYSVYEG
                     FGSGITVPGYGFVLNNRGALFSLAPRSANVIAPRKRPFQTILPGFLMRAGEPVMAFGL
                     MGGGQQAQGHAQVLVSMIDLGANPQAASDAARFAHWQASNTLNLETGLYEMLGPQLRA
                     LGHDVVAGNGLDMGGFQAIWFDPDRKAPHAAGSTSQAIAGVYRAASDHRKDGCAAGW"
     gene            complement(13724..15700)
                     /locus_tag="VFB37_11320"
     CDS             complement(13724..15700)
                     /locus_tag="VFB37_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO23082.1"
                     /translation="MRKLALGALTASVALGAAVVDGQAPTAEQSPSPTRLVHRFLDVE
                     ISPDGKWVASVEGNVPLNGYYPAVRQLVVRTVQGKSETRIDLPCGQVEQCWPGSPTWT
                     ADSRHLSFTLRKPGSHAYSIYTVEPGGGGLTDLIDFSGTLIDLRYMPDGRLAMLATEG
                     ARKEVGATEAGTPVAGDLDQAPPEQRIALLDHGSLQWVSPANLFVYEYDWRPDGHGFV
                     GTAAPGNGDDHWWTAKLYAFPAAGASAQILYSPDNARQQIAMPKVSRDGRSVAFIGGI
                     MSDFGSTGGDLFVLPIEGGAAIDLTHDMPFSATSLAWGCKGHLMAKLLAGDKSEIADM
                     GTGHGAITPHVLWSGAESLSRHDAGVSWACSSSGVTATAHDSFTAPPEIEVGVIGRWH
                     DITNVNAGMGLPARVQSLHWKNEGFDVQGWLLLPEHVSGKIPLVTIIHGGPASAAEPT
                     FRGPGLEERLLQQGYALFHPNPRGSYGQGERFTAANVRDFGHGDLRDVLAGIDAAEKA
                     APIDETRLGLTGGSYGGFMTMWAVTQTHRFKAAVAAAGISDWISYYGENGIDAWMIPY
                     FGASAYNDPDVYAKSSAINYIRNVRTPTFEYVGERDIECPAPQTQEFWHALKDLGVPT
                     SIMIYPGEGHGLREPAHNEDALRRTLDWFDLYLK"
     gene            complement(15701..>16609)
                     /locus_tag="VFB37_11325"
     CDS             complement(15701..>16609)
                     /locus_tag="VFB37_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23083.1"
                     /translation="SDRSIDLNGVILLSQILSFDLSADRPTSNPGVDLPYQTVLPTYA
                     ATAWYHHKLPAEHQNLEAFLSEVEQFAMGDYARALAAGSDLSANDRAAIAEKLHQYTG
                     LPVDYILRADLRIDGGEFRQNLQEDGSLTTGRLDTRFSGPDIDPLSQRADYDPQSTAL
                     GSASVSAFNEYVRKDLHYGEGKIFKPSIRTFRTWNFSHQLPGQSEAPMSTRQGTNVMP
                     DLANAMKLNPNLKVQLNAGYFDLATPFYQGIYEMHHLPIPPQLQSNIEYRFYESGHMV
                     YAKESSLKQLHDNVADFIRRTSNTGS"
BASE COUNT         3203 a         5271 c         5445 g         2690 t
ORIGIN      
        1 gctgtccgat gaccccactg tccaccttgt agcgccacac cagctcgcca gtgcgcgcat
       61 tcaccgcctt gaaccagcca tccatggttc catagaacac cagcccgccg ccggtcgcga
      121 ccgtgcccga ccacaccggg aagttttcct tcgcttgcca gactgcatgg cggcgtaccg
      181 gatcccaggc cgtgacggcg ccgcgattgc ctccgggccc gggcttcata tggacctgcg
      241 ccccgacgta tggtgtcccc tcgatgtagc tcacctgcat gtccatccaa tccatgcaga
      301 tgttttcgtg cgggatgtag atgagaccgg tctgcgggct gaagctgctc ggctgccagt
      361 ccttcgctcc ggaagcggtg ggacagatat cggtcacctg ctcgccgagc ttggtctgct
      421 tggcgggatt gatgatcagc cgaccggtct tcagatcgac accgcgcgag gaattgacgg
      481 cgctgaacgg atcggcggag agcacttggc cgctcttgcg gtcgatgaca tagatgtacc
      541 cgttgcgatc gaaatggatc agcaccttgc gcatctgacc gccgaagggc atgtcgagca
      601 ggatctgctc gttgatgcca tcgtagtcat gctcgtcgtg cggcgtgaac tggtagaacc
      661 agcgcgcgct gccgttcgcc ggatcgcgag cgaaaatgcc cgctgtccac ttgttgtcgc
      721 cagggcgctg ctcggcgttc caggggccgg ggttgccggt gccgtaatag atcgtgtcga
      781 actccgcatc atagttcggc cagccccaga cactgccgcc gccgatcttc caggcctgcg
      841 gcggccagct cttcacgccc agatcggtgc cgcgatccga ggcgtatgcg ggtttgaatt
      901 ccgagccgat gaggacctcc gcgtccggcc cggtgctgta ggccttccac agcagcttgc
      961 ccgagctctc atcgagggct gtcagccagc cgcgcacccc catctcacca cccgagttgc
     1021 ccacgtacac atggcccttg gcgaccagcg gcgccatggt gatcgactcg ccggtgttga
     1081 tgttgccaag gcgcgtgcgc cacacggctt tgccggtctt cacatccagc gcaatgacct
     1141 ggccatcgag cgtagtgaag atgtacttgc cgtcatcgac gctcccgccg cgattgacga
     1201 catcgcagca ggcaaccccc tggctggcgg gcaccggctt cggggcgaat ttccacttga
     1261 gcggcgcgcc gggcttggtc agatcgagcg cgtagacgta gttgggatat gccgtcacga
     1321 cgtacatcgt gttatcggcg acgatcggcg cagcctcctg acccttgttc acgtcgaggg
     1381 aaaaggtcaa cagcggctgc agattcttga cattctcggg cgtgagctcg gtcagcgagg
     1441 agaagcgggt cgaggcgaag ttcttcgccg gcatcgtcca ttgaccatcg tcctcgggag
     1501 cgttcgcgcg gctggccgcg gccttgccgg catcgggcgt cggggccgcc agggcatgtc
     1561 cgatctgctc ggggctgaag acctcatccg ccggcaccgg ttcgggcacc gtctgcgcca
     1621 gcagcgcgca cggcatcagt gagagtggaa tcaacagtgg cagcagtctc atgtgtcagt
     1681 ccccgccagt cagccggcgc attgcggtaa tacgatgagt gccaccgcag tgaaggcaat
     1741 ggcaatggcg aagccgcctc cggccgccat gccccagacc gcgagaaagc gcgtccggca
     1801 cagcccgatc ggcagcccca gctgggcgac ctggcccgac tggccatcaa tgcgttggcg
     1861 gatgtgtgat gaagcgagaa acgcccccac cgcaatgagg aaggccaccg ccgagatccc
     1921 gacgacgacg ccccaggtcc agccgtaccc ttccagaggc gcaaaccggc gctgatcctt
     1981 cggaaagcag gcccagctcg agaagccata gccggcgcag cattgcgcga accaggcaag
     2041 cggtccaccc agcagcccga acaggctcaa tgtcagcgat gcgccgctcg ggccgggcgt
     2101 caccggtgcg cgcaaggcag tctccggcgc aatcatctgc cgaatccgag gtaaggagtg
     2161 atgtagtaag tcatgaagat cagcagccag acgagatcga cgaagtgcca gtagatcact
     2221 ccggccgaga cgctcagctg gcgtcgcggg ctgaaatagt cgagcgccgt ccacagcagg
     2281 atggccgcca acacgagcac accgacgatg acatgcgcga tgtgaaaacc ggtcgtgatg
     2341 taataaagcg aggcgtagct cgaatcgctc agccggaatg gccgcgcata ccactcgaag
     2401 ccctgcacca gcacgaaaac gatgccgagc accagggcga tgctgaagcc gatgagcgcc
     2461 cggctccgct gccgacgcaa tacccccgtt tcgccgtacc aggcaaatac gctcgagacg
     2521 agcagcacga tggtattggg gagcgcgagc ttgagcgtcg gatgggactc gagcagccag
     2581 cctcgggggg aaaccgcgcc ggtgtagtaa taggcaaaca gcaggtaggc gaacagcgcc
     2641 gcctcagtcg caatcagcgt cccgacgccc cacagcccca gtccgcgtcg gcccacaggc
     2701 ccaacaggca atttgccggg ttcggcgagc aggacaggcc ggtctcgctg cgcccggcgc
     2761 atctcgagct cgctcggtgc caggcccgca ccgttcacgg attgacctcc cgctcccgca
     2821 actcacgccg cggccacaac cagaaaaggg tcgcgatcgc gagcaccacg gtgccgatga
     2881 tcacgatgct ccagagcttg agcagcagtc cgacgaatag gagtgcggtg ccgagcgtca
     2941 gtacgaaagg tgcggtgctg tcctccggca tctcgaggat catgtcgggc gtggcatcca
     3001 tgctgctggt gccgatagcc tccttgctgt gcgactcgag cagcatgccc tgatcgacgc
     3061 aggagactgc gccggactcg cgcagccgac cctcccagag cgggtaccgc gatgcgaccg
     3121 tcgggatcac ggcaaagtta tagggcggcg gcggtgagga cactgaccac tcgagcgagt
     3181 aggcgtccca ggggttggga cctgcgagcg ccccgccacg cagacttttc aggacgttga
     3241 cgatgaacaa cagcaccccg agcgccagca cgaagctgcc gatcgtcgtg atcatgttca
     3301 ggctgtccca gcccatgccg tcctgatagg tatacacacg cctgggcatg ccgagcaggc
     3361 cggtcaggtg catgggcagg aaggcgaggt tgaaaccgat gaacgtcagc cagaaattcc
     3421 agcgcccgag acgctcgtcc atcaatcgcc cggtcatctt cgggaaccag aaatagaccg
     3481 cgcccagaac cgggaagaca ttgatcccga tcagcacata gtggatgtgc gctacgacga
     3541 agtacgtgtc ggtcaattgc cagtccaccg ggacgctcgc ggtcatgaag ccggagacac
     3601 cgccgatcac gaacatgagg atcatcgagg agaaatacag gaacgctgtg gtgatcacgg
     3661 ggcggcccag ccagagggtg gcgagccagg caaacacggc caccgctgac gggaagacaa
     3721 tggcaatcga tgcgccggag aagaaggaca gtccgagccc gggcagaccc gtggcgaaca
     3781 tgtgatggac ccacacccca aatcccagca ccatggtggc gaccgtggag agggccacga
     3841 tcccgtagcc caccagcggc ctgcggcaga acaccggcag agcgtgcgag accatgccca
     3901 tcgccggcag cacgatcgca tacacccagg gatgaccgaa gatccagaag aggtgttgcc
     3961 acagcaaggg ctgaccgccg ccggccggat cgaagaagtg ggcgccgaac tggcgctcga
     4021 gccacagcag aaagaaggcc aggctcactg ccggcaccac cacgagattg gctgcgctcg
     4081 ccgtgagcgt gccccagatg aggaccggca tccgattcag cgccatgccg ggcgcgcggg
     4141 tgcgcatggc ggtcacgatg aagttggctg cgccgatcgt ggtcgagatg ccgagaaaca
     4201 ccatgccgag agagtagaag tcctcgttga gccccgggtt gtagtcccgg tcggcatacg
     4261 gcacgtaatt gaaccagcca tcgttgggcc cgttgcccgt gagaaagccc acgtaaagaa
     4321 agatcccact cgcaagatag atccagtagc tcagggcgtt cagcctcggg aaagccatgt
     4381 cgcggctgcc gagcagcaac ggccacaggt agttcgagaa gcccgacaaa atcggcgagg
     4441 catacagaaa gatcatcgtc atgccatgcg tcgagaacag ctgatcgtac tgttccggcg
     4501 tgagcagact gaggttggga ccggccagct gcaggcgcat cacgagcgct tcgatcccac
     4561 cgagtgcgag gaaaatgaaa gccgtcacga tgtagcggat gccgatctgc ttgtgatcga
     4621 cggtccccag aaacccttcc agtcccgggg aggtttccca gagccgcttc agcctgcttt
     4681 catgctcggc agtggtgggc cccacggccg gcgcgcgaaa gtccgccacc ctcattgcag
     4741 actctccagg taggcctgga cgtccgagag ctgttgaccc gaaagatact gattgggcat
     4801 gagcgtaccc ggcttgaact cctgagcatt ctggatccag ccggcgaggt tgcccggtgt
     4861 gttaggcaac agccccgccg ccagggtgcg ccggctcatg aggtgagtca gatcgggggc
     4921 ggcgaccgct cccgcctgag tgccgcgcac ctggtggcag agcccacagc gatactcgac
     4981 caccgccaat ccccgctgct gcgcggaagt ctggggagcc cctgccgggc gcagctgggc
     5041 ggcgcgccag cgatcgaaat ccgcctgagg ctgcgctacg acttcgagcc ccatgtgggc
     5101 atgctgagcg ccgcagtact ccgtgcactg acctcggaag cggccgggcc cgcgcgcttc
     5161 catccagctg aggttggtct gtccgggaat ggtgtcggtc ttgcccgtga gcgccggcac
     5221 ccaaaagctg tggatgacgt cgccgccgtg cagtctcacg cgcaccggta cgccgacggg
     5281 gatgtggatc tcgttagcgg tctcgaagga gcgatcgggc tcggcggcgt cgtagcgcac
     5341 ttcccaccac cactgatggc cggtcacatc gagtgttaag ccgggaggcg aggggggtcc
     5401 ggaggtggcc gctatggcga ccatcgtcca gatgagcgtg gcgagcaacg gtacggcgga
     5461 aatcaacaga ccgatgctga tccagcgggc gccgcttggc ccgggttcaa cctcgacgga
     5521 tcgggtctct gccgcaccgc cgctgccacg cgcacgtcgg atggcccccc aaagcagcac
     5581 ggcaatgatc acgcacacca gcaccgagat gatcagcgtg aaccaggtca ggggcagaat
     5641 cgttgcggcg cgatggccgg cggcagtcag ataggtgagg ggggtcgcgc cgctcatggc
     5701 gagccggctt cctcgagcac cctccacaga cggcccaagg agcgcagggc agcacacaga
     5761 aacagtgcaa tgcccggcac ccacatgaag acgccgccaa gttgctggtc gtgcagcggg
     5821 gtgaaccccc aggcggccgt cgtcgtcagg tgccagagga acatcggatg ccccgcgaag
     5881 gtcaagaccg caccgagcag gcccatctgc atggatgtga gcgcgccggc ggctaacaca
     5941 tccgcggtcc tgtcggatct gtgatgcaga agctcgcgcc acagcaggac tgcagagcca
     6001 aagagcgtca ggtgcatcgt ccagtagatc ggagtcgagc tgaaggtcag gtcataaggc
     6061 accggcatgt gccagaacca cagtgccagg aagaatgcgg ctgcactccc ccacagaccc
     6121 agtgcgccgg tgcgatgcga ggctcgtggc cacgccagag cgatcaacgg cgcagcgagc
     6181 aagaccagaa tcatgtgctg cgcgacgcgt gcgctgaaaa gggagaccga cagtgcgcac
     6241 agcggagaca acagcgctgc agcggtaatg agccagccgc ctgcggcgta ccaacgcgtg
     6301 tgcgtacccc gtgccgccca cagatgagtg ccggccagca ccaccaacgc caaaatcagg
     6361 acggggtcga tgttgaatcg ggtgagcagc tcacccggag ctggaggcga accgcagtaa
     6421 ggtaacactt ccatggccgc tgtctcgtgg ggcatggccg ctgtctagta aggcggtaca
     6481 acggcgcgcg acgaccaaat atcctccgtc cctgtgaaat atttctttca tgccatggaa
     6541 gaatgtacct gcaacagcca agctccggtg tcaggtggtt ggaccaacac cccggcttcc
     6601 aaccctgact gcggacacgt ttcgtacatt tcttgacaga acaccgtggc gccatgcgag
     6661 ctgcgtcgca gatcccgaca gggcagctgt ggtgaggtgc gggttatacc tgcacgcacg
     6721 ggtcggccag tgcatctggc ccggttcttg cctgtcctca tgagcgattt accggaatgt
     6781 aacaaggatg aatttcgaag acgctctgaa tatcggactg cacgcctacc gcgtggagcg
     6841 agtcgagcac ggcgatgcgg tggaataccg cgccacgctc cccgactatc ctcagtgcac
     6901 gggatgcggc ggtacggcgg aggaggcggt gcgcaccgcc cgccaccagc tgactcagct
     6961 tcgtgacagc caggcggctg tggcggtttg tgatctggag gatgagcgct cggaccggct
     7021 gcgcgaggcc attcgcgcgg tcgtcacgcg ggcactgatc tgaaggcggt ttagccggcc
     7081 gccggcgggg cttagcttcc cgtccggttc ggatcgcgcc atgcccgctc gaagggcagg
     7141 cgccaggcgc tcggtgcgac gagttggtga atggcattcg ggccccagga gccgggcgca
     7201 tagtggcgca ccggcggcgg tgcctccagc aggggcatcg agacttccca cagccgctca
     7261 atcccttccg cggtcgtgaa cagtgtgtga tcgccgcgca tggcatcgag aatcaaccgc
     7321 tcgtaggcct cgagcacgtc tccgacgagc ccggtatcgt gcatggcgaa ctgcaggctg
     7381 agtttgtcga gacgcatccc cgggccaggc cgcttgccgt agaaggacaa tgacatcttg
     7441 gaggcatcgg cgaggtcgaa ggtgaggtgg tcgggaccct gcgcaccgac tccggagccg
     7501 gggggaaaca tactcttcgg cggctcgcgg aaagcaatgg agatgatccg ctggccttcg
     7561 gcgaggcgct tgccggtgcg cagatagaac ggcactcccg cccagcgcca gttgtcgatc
     7621 gagcatttca gcgcgatgaa ggtctccgtc tccgaatcgg cactcacccc gggctcggag
     7681 cggtatccgg tgtactgccc gcgcaccaca tcggtcggct ggatgggcag catgctgcga
     7741 aagaccttgt tcttctcctc gctgatggga gcgggctcga gtgcggtggg cggctccatg
     7801 gccatgaagg caaggatctg gaacaagtgg gtcacgacca tatcgcggta cgcacccgtg
     7861 gcctcataga aggccgcccg ggtgccgaga ctgagcgtct cgggcacatc gatctggaca
     7921 tgatcgatga agttgcgatt ccagatgggc tcgaacaggc cgttcgcgaa gcggaaggcg
     7981 agaatgttct gcgccggctc cttgccgagg aaatggtcga tccggaagat ctgctcctcg
     8041 gcgaatacct cgtgcagctt tgcattcagc gagaccgcgc tggccaggtc cgtgccgaag
     8101 ggcttttcca tcacgattcg cgaacgcggc acgagctcag cctcacccag caggcgcacg
     8161 gccgagagcg ccgcccccgg gggcacgctc aagtagtgca ggcgctcgca atcgccgctg
     8221 aaggcctgct cggcttttcg caccgcaccg gcgagcgcgg cggcgcccgc cgacagcggc
     8281 acatagtcca agcacctggc aaagacgtcc cagtccgcct caaggatctt gcgggtggag
     8341 aattcattca gcgcagcgcg cgccacgctg cgaaagccat ccgcgtcgag gctatcgagt
     8401 gacacgccga caatgcgaca ggcaggaatg aatcccgccg tggcgaggta aaacaaacct
     8461 ggcaggagct tgcggcgggc cagatcgccc gtggcaccaa cgagcaccag cacctgcgcc
     8521 gagcgcggac ccactcccgg aagaaccttt gccaccgtgc ctacccttag gttatggagc
     8581 gcgctgccgg gaggcggtcc gcggcaggcg cgcacatggt acgcacgctt tgttaacccg
     8641 gatagtcgat ggtgtcgcga atcagctgca aaagtcccgt ccccttggcc acgcggcgac
     8701 cccccgcctg cgtcaggtgg atctcatccc ggtacagcat ctcgcccgac tgcaactgct
     8761 cggaacaata gcgcgagttg cacagagccg ggatggggtc aacgagcacg agggacggaa
     8821 accggtcctt cacttcctcg agagcagtac tgtaggccga gcgatgcaac tccagggcgc
     8881 cgcgcgcgag gaaggccgag caacgggtgc ccggcaatct gaatggacgc agatagcagg
     8941 acggtgagct ctgaaattcg ggaatctcac gcacgtacac aacgcgggag gtagcctgca
     9001 gcgcaccgag taggttttcc atgccgcgct tgaaagcggc ccgcttcgca ctcgcatcct
     9061 tcggcgagtt ggcatcgaag aaccgggtgc ccgcgccgac cagcaccacc agccgcggtc
     9121 gggctttctg cacgtactca acgaactcgt tccaggtttt gttgcagttc tgttgcgcag
     9181 actcggacgg gtacaagccc tgcacggagg cgttcagggt aggcgggcag ccgccgcgcg
     9241 ccagcatcat aagcgagtag cttttgccga gtgccgcagc ggctccctcg taaaccgcct
     9301 cggtctgcga gtcacccaga aaaaccacat cgggtggctg ctcgctcgtg cgcttgcaat
     9361 agttgaatgg gatatgcgta tccaccgtct cctcgcacag cgggtcagtc ttggcctgcg
     9421 ggcgtatggc ggcgacctgg cgatcgaatc ggtgcccaaa ccccgcggca tggaagaccg
     9481 tcagccccgc cagccccgcc accaccagtg agcccagcag cgccggactg agcggccgcc
     9541 agtggcctcc tgcgcggatc ggcttctcga tgaggcgata agtcaggatc gccagcccga
     9601 aacccaacac gacgcaggcc cagcgcgtca gcagattggg cgtgcccgaa tgcagaatcg
     9661 cggcaaagga cagaatgggc cagtgccaca gatacagcgg gtagctgatc agtccgacga
     9721 acaccagggg tggataggcg aggatccgcc gctgaaaacc gcttttcccc ggctgcgcga
     9781 cgatgcacag cgcaccgagt gttggcagaa gactcggcca tcccgggacg ccactgtgac
     9841 tgttgcagca aaacacagcc acgagaatca tgctcaaacc cgccacgctc agcagtgagg
     9901 ctaccgccgc accccacgca cgcatgggcg gtggccggcc cggtgagccg cagctgagcc
     9961 acgccatgaa acatccgagc ccgagttccc agaagcgtga gagcgggaag tagaaggcgg
    10021 ccgtcggatg atgccgcacc agaagcagat cccaggcgaa ggagccgccg gtgaagagcg
    10081 cgatgaccca tccggtcgcc agccggcgcc tgctcagaag cagcagcagc agcggccaga
    10141 gcagatagaa ctgctcctcg atcccgagtg accacaggtg caggagcggc ttcagctgcg
    10201 tggcgggggc gaaatagccc gtctcggtcc agagcgcgaa gttcgacacg aaggcggccg
    10261 cagctgcgac ctgcctgccg agatagccgt actcctcggg caggagggca taccacccga
    10321 aggccaggca ggcagccaga acgatgatga gggcgggcag gatgcgccgg atgcgacgcc
    10381 tgtaaaaatc acccagactg aatgtagagc gctgcagttg accgaagatg aggctcgaga
    10441 tcaggaagcc cgagatcacg aagaacacat ccacgccgaa gaatccgccg gggaaaacgg
    10501 ccggaaaagc gtgatagccg attacagcga ggacggagac ggcgcggagt ccgtcgatgt
    10561 cgggacgata ggcgagcttt gtcgcgggtc gttctgccat gggggtcatg ggcttgcaag
    10621 gatggtgccc gcccgccaag ctcgtgcatt gcacgtttcc gagcggctgt gctccacttc
    10681 gccgcccctg tgcacatcag aacgaggaga ctgaatctca tgatcccgca atcgaggtcg
    10741 tttctgcccg gactctgcct ggcgctgctg gcggcccttg cgccatgcct gggttttgca
    10801 gccgatacgg actatcacgc ccgcctcgca cgcatcctgc agcgaacgcc gctcatcgat
    10861 ggacacaacg acctaccgtg ggagatccgc acccgcatgc acgggcagct tgccgggatc
    10921 gatctgaaaa gcgacacctc gaagctccgt gcaccaacag gtgaggcgcc gttgatgacc
    10981 gacatcccgc ggctacgcgc cggcatggtc ggcggtcagt tctggtcggt ctatgtgccg
    11041 gcggattaca agggtttcga ggcagtccag gccaccgtgg agcagatcga tctcgtcaag
    11101 cggatggcgg cacgttatcc ggcggacttc gagatggcat acacggccgc cgatgtgcgc
    11161 cgcatccaca aggccggcaa gatcgcctcg ctcatcggca tcgaaggggg ccaccagatc
    11221 gataactcgc tggccgtgct gcgacagatg tatgagctcg gcgcccggta catgacactg
    11281 actcacgtgc tgaccacggg ctgggcggat tccgcgaccg atgcgcctca acaccacggg
    11341 ctcacaccct tcggacagga agtcgtgcgc gaaatgaatc ggctcggcat gttggtggac
    11401 ctcagccacg tctcgcccga tacgatgcgc gccgccctgc aggtcaccca ggcgccggtc
    11461 atcttttcgc attcctcggc acgcgccctg gtcgatcatc cgcgtgacgt gcccgatgac
    11521 atcctgaagc tggtggccgc gaacggtggt gtggtgatgg tgaacttcgc tccggagtac
    11581 gtctccgagg cgcgcaatca gtgggaggcc gaccgggcgg ccgagcaggc gcgctacagc
    11641 agcccgccct acggcggcct gtatatcggc cagcccgatc gggccaaggc cgcgttggag
    11701 aaatgggaca gcgcccaccc ccggccggtc acgacactcg cccaggtcgc tgaccacatt
    11761 gatcacgttc gccaggtggc cggcgtggat tgcgtgggcc tgggatcgga cttcgacggc
    11821 attccggata cgccaaaagg cttggaaggc gtcgatcact atcccgcgct gctcgaagag
    11881 ctgatgcgcc gcggctggtc ggatgcggat gtcgccaagg tcgcaggcga gaacgcgctg
    11941 cgcgtgctgg cgggtgcgga gcgcgtggcc gccaagttgc gcagcgagcg ggggccgtca
    12001 gaggtgctgt tcgaacccgg gcactgagct caccagccgg cggcgcagcc gtccttgcga
    12061 tgatcggagg cggcccgata gaccccggct atcgcctggc tcgttgagcc tgcggcgtgc
    12121 ggcgccttgc gatcgggatc gaaccagatc gcctggaagc cgcccatgtc caggccattg
    12181 cccgcaacta catcgtggcc gagcgcgcgc aactgcgggc ccagcatctc gtagaggccg
    12241 gtctccagat tgagggtgtt ggaggcctgc cagtgcgcga agcgtgccgc atcgctggcc
    12301 gcctgcggat tggcgcccag atcgatcatg ctcaccagca cctgcgcgtg cccctgtgcc
    12361 tgctgtccgc cacccatgag gccgaaggcc attaccggct cgccagcgcg catcagaaaa
    12421 ccgggcagaa tcgtctggaa aggccgcttg cgcggcgcaa tcacattggc actgcggggc
    12481 gccagtgaga agagggcgcc acggttattc agcacgaagc cgtagcccgg cacggtgatg
    12541 cccgagccga acccctcgta gacactgtag atgaaggaga ccatgttgcc ccagcggtcc
    12601 gcggcagcga tgtagacggt gccgccaacg gggtctccgc ggggctcagg ggtgcgcgcc
    12661 gcacgcgggt cgatcgccgc gcagagctcc tgcgcatgcg atctcgagag caggtgctcg
    12721 atcggaacat tggtgaaagc cgggtcggcg ttgaattcgt acagatctgc gtaggcaagc
    12781 ttcttggcct cgaccagcag gtgccagtac gcgctcgagc gcgggccgag ctccgcgaga
    12841 gtcactccgg acttctcggc ataggcctcg agaatgttga ggatctcgag cgcggcgaag
    12901 ccctgcgtgt tgggaggcat ctcgaagaca tcgaacccgt gatagtgggt ggacagcggg
    12961 gcctcccagg tggctcgagt cccggccaga tcctccagcg tgagcatgcc gccgagcgcc
    13021 tgggacttgg ccacgattgc ccgcgcgatc tcgccttgat agaaggcctc cttcccccgc
    13081 gattgcaaca cgcgcaaggc gcgtgcgaga tcggggttac ggaaagtcga gtagaggcgc
    13141 ggcgcgttgc cgtcgatcag gtagaccgcc gccgaatcgc ggtcggctcg gagcgtctcc
    13201 acgtcgcgga cccagtcctc gtagatgcgt tccgtgagtc cgaagccttc ctcggccaag
    13261 gtagcggcgg gctccagagt ctcgcggaag ctcagggtac ctgcacgctt cagcagcacg
    13321 tcccaggcat cgacagctcc cggaacggtg accgcatcga tggagcggcg tggcatttcc
    13381 ttcgtatgac cgcgctcggc gaggcgctcc ggcgtggcac cggcggcagc gcgcccgctg
    13441 ccattcaagg caaccagctg cttctcccgg gccacccatg ccaggacaaa cacatcgccc
    13501 cccaggcccg cgccggccgg ctccaccacg ttcaacaccg ccgcggtggc gaccgcggca
    13561 tcgaaggcat tgccgccctg cttcaggatc tgcaaccccg cctgcgcagc cagaggctgg
    13621 ctggtggcga cgatcccatt gcgcgcgagg acctccgagc gagtctgacc cagccaaccg
    13681 ccggcccggt cgccgcgcgc ggcggtgaac tcacgcgcgg ccgtcatttg aggtaaagat
    13741 cgaaccagtc gagtgtgcgg cgcagtgcat cctcgttgtg cgccggctcg cgcaggccgt
    13801 gcccctcgcc cggatagatc atgatcgagg tcggcacgcc gaggtccttg agcgcgtgcc
    13861 agaattcctg agtctgaggg gcagggcatt cgatgtcgcg ctcaccaaca tactcgaacg
    13921 tcggggtacg cacgttgcgg atgtagttga tggcggagga tttcgcgtac acgtccgggt
    13981 cattgtaggc agaagccccg aagtacggga tcatccaggc atcgatacca ttctcgccgt
    14041 agtaggagat ccagtcgctg attccggcgg cggccacggc cgctttgaag cgatgggtct
    14101 gcgtgaccgc ccacatggtc atgaagccgc catagctgcc tccggtcagc cccaggcgcg
    14161 tctcatcgat cggagcggcc ttctccgccg catcgattcc ggcgagcacg tcccgcaagt
    14221 cgccgtgacc gaagtcgcgc acgttggccg cggtgaaacg ctcgccttga ccatagctgc
    14281 cgcgcgggtt gggatgaaac agcgcatacc cctgctgcag cagccgctcc tccagccccg
    14341 gacccctgaa ggtgggctcg gccgctgagg cgggcccgcc atgaatgatg gtcaccagcg
    14401 gaatctttcc gctgacatgc tcgggcagca gcagccagcc ctgcacgtcg aagccttcgt
    14461 tcttccagtg caggctctgc acgcgcgcgg gcaagcccat gccggcattc acgttcgtga
    14521 tgtcatgcca gcgcccgatc actccgacct cgatttcagg cggggccgtg aaggaatcgt
    14581 gggccgtcgc agtcactccg gaggacgagc acgcccagga aacgccggca tcgtgtcgcg
    14641 agagggattc cgcgccactc cagagaacat ggggtgtgat tgcaccgtgg ccggtgccca
    14701 tatcggctat ctcggacttg tcccccgcca gcagcttggc catgagatgc cccttgcagc
    14761 cccaggcgag ggaggttgcg gagaagggca tgtcatgtgt caggtcgatc gcagcgcccc
    14821 cttcgatcgg cagcacaaac aggtctccgc ccgtggagcc gaagtcgctc atgattccgc
    14881 cgatgaaggc gacgctgcgc ccatcgcgcg aaaccttggg catggcgatc tgctggcgcg
    14941 cgttgtcggg actgtagagg atctgggcgc tcgcaccggc ggctgggaag gcatagagtt
    15001 tcgccgtcca ccagtgatca tcaccattgc cgggtgcagc cgttccgaca aatccatggc
    15061 catcgggccg ccagtcgtat tcgtacacga acagattggc gggtgagacc cactgcagtg
    15121 agccgtgatc caggagggcg atgcgctgct cgggcggggc ttgatcgaga tctccggcca
    15181 cgggcgtgcc cgcctcggtc gcgcccactt ccttgcgggc gccctcggtg gccagcatgg
    15241 cgaggcgccc gtcgggcatg tatcgcaggt cgatgagggt gccgctgaag tcgatcagat
    15301 ccgtcaaccc gccgccaccg ggctcgacgg tatagatcga gtaggcgtgg ctgccgggct
    15361 tgcgcaaggt gaagctcaga tggcggctat cggccgtcca ggtgggagat ccaggccagc
    15421 actgctcgac ctgcccacag ggcaggtcga tccgggtctc actcttgcct tgcacggtcc
    15481 ggacgacgag ttgccgaaca gcgggatagt agccgttgag gggcacattg ccttcgacgg
    15541 aggcgaccca cttcccatcg ggtgagatct cgacatccag aaagcgatgc acgaggcgcg
    15601 tgggggaggg gctctgctcg gcggtgggag cttgtccatc cacgacggct gcgccgagcg
    15661 ccacactggc cgtcagggca ccgagggcaa gcttgcgcat tcaggagcct gtgttgctgg
    15721 tccggcggat gaagtccgcg acgttgtcgt gcagctgctt gagcgaggac tccttggcat
    15781 acaccatgtg gccggactcg tagaagcgat attcgatgtt ggactgcagc tgcggcggaa
    15841 tcggcagatg gtgcatctcg tagatgccct ggtaaaaggg agttgccaga tcgaagtagc
    15901 cggcattcaa ctgcaccttg aggttggggt tgagcttcat ggcgttcgcc agatccggca
    15961 tgacattggt cccctgacgg gtggacatgg gcgcctcgct ctgcccgggg agctgatgag
    16021 agaagttcca ggtgcggaac gtgcggatgc tcggcttgaa gatcttaccc tctccgtagt
    16081 gcagatcctt gcgcacgtac tcattgaagg ctgacacgga ggcagatccg agggctgtgg
    16141 actgcggatc atagtcggca cgctggctga gcggatcgat gtcaggacca gagaagcgcg
    16201 tgtcgagccg gccggtcgtc agactgccgt cttcctgcag attctggcgg aattcgccgc
    16261 cgtcgatgcg cagatcggcc cgcaggatgt agtccacggg aagcccggtg tactggtgga
    16321 gtttttccgc gatcgccgcg cgatcatttg cgctcagatc cgagccggcg gccagtgcac
    16381 gcgcataatc acccatggcg aactgctcga cctcgctcag aaaagcctcc agattctggt
    16441 gctcggccgg cagcttgtgg tggtaccagg cggtggccgc gtaggttggc agcacggtct
    16501 gatagggcag atccacgccg gggttgctgg tgggccggtc cgcgctgaga tcgaagctga
    16561 ggatctggga gagcaggatg actccgttca gatcgatcga gcggtcgga
//
LOCUS       DASRHN010000281         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_95483, whole genome shotgun sequence.
ACCESSION   DASRHN010000281 DASRHN010000000
VERSION     DASRHN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95483"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFB37_11330"
     CDS             complement(<1..497)
                     /locus_tag="VFB37_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO23084.1"
                     /translation="MRSSLALVSLTAIVALTLSACARNEAATPANMPPPQVTVAQVLT
                     KPVTEFDEFTGRFEAVDRVELRPRVSGYIASINFTQGKEVKKGDVLLVIDPRPYQADY
                     NRAKAQLAQARTQRELAKSEKDRAVKLLAQHAISQEEYDTRTAGSEQADANVEAAQAA
                     LDSSAL"
     gene            complement(1138..1416)
                     /locus_tag="VFB37_11335"
                     /pseudo
     CDS             complement(1138..1416)
                     /locus_tag="VFB37_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(1792..2148)
                     /locus_tag="VFB37_11340"
     CDS             complement(1792..2148)
                     /locus_tag="VFB37_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HZO23085.1"
                     /translation="MSSNVKSDKRRILGYAAAMWLACTLVAFNANADDGVRSETVKFA
                     DLNLDTPAGVEALYRRIHAAAQRVCDQPFGQESGIRPCMTKAESEAISKVNVPLLTAF
                     YQKKSGILPPTVTASR"
     gene            2366..4792
                     /locus_tag="VFB37_11345"
     CDS             2366..4792
                     /locus_tag="VFB37_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO23086.1"
                     /translation="MLFQTDRARSAEPMTSEEDRLRAARSTEIASRGNSPRLAVGQLL
                     GGRYRLERELGEGGMGVVYLAADEQVPGEKFAIKVLKEELHPEALALLREEVRKTRKL
                     SHPNIVDVHSVNVEGRRLFVLMEYLEGKSLDMLLDEEFGRGMPFSHAWPIIKDVGAAL
                     GNAHDHSVIHSDLKPANVLLTTSGRTKLLDFGIARVTRGPLLHARSGTRALTPAYASC
                     EMLEGREADRRDDIYSFACVIYEMLRGERPFGQLTALEARNASAPVPPLQGMSREQNA
                     ALRQALAFDREARTPSVEQLLAGLTGSQPRARANALLTGAIFAAIAALGIIFWVAARL
                     WIPRPSIVAAPVTSPEAERAAVSPKSIAVLPLLDLSTGHEQEYFAEGLTAALIDRLTL
                     FPGLSVTGQTSSFALKDSKEDAPTLARRLRVEYLLEGTVRGSAEQLRVSARLIRAVDG
                     FEVWSQVFDRRGSEILKLEEDIARAVAADLRGSLLDTPVPVVPTTRSLEAYRLLLRAD
                     QLWRQEFATDDERALIEAALQKDPGYAAAWVELGFWWYDSMLVGKTETQTAVPRARGA
                     FDRALALDKNSSRGYIGLAWLQMTQHDWVGAQRSIERADALDPASLSVLTAHGELARS
                     AGRWAEAIRLEQAREQRDPLSVGTKFVLAQSHLCGGEYANAEDILRQTIAENPRYPGA
                     HALLARALAARGEAAAALRVVALEPDEPARLAGLASVQSDLGRPDASDEAITQLRTRY
                     SQTLPGLTGLAYAYRNQLELAFPWLQRAVEQRQPEFAASLACTPEPAFASLHADPRFA
                     ALRGGLGFKR"
     gene            4885..5169
                     /locus_tag="VFB37_11350"
     CDS             4885..5169
                     /locus_tag="VFB37_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23087.1"
                     /translation="MKKKDNGLDELLALLRANPELIKELIFDPANVKRLLKSEAAREL
                     VIGQDTEAFLRYIAGPDDGYPIAQCLKQTKYLCAKGTRHVACGGGTKSSK"
BASE COUNT          926 a         1682 c         1709 g          929 t
ORIGIN      
        1 agcgccgagg aatccagggc cgcctgcgcg gcctcgacat tggcatccgc ctgctcgcta
       61 cccgccgtgc gggtgtcgta ctcctcctgg gagatcgcgt gctgcgccag cagcttcacg
      121 gcgcggtcct tttcggattt cgccagctca cgctgggtac gggcttgcgc cagttgcgcc
      181 ttggctcggt tgtaatcggc ctggtaggga cgcggatcga taaccagcag cacgtcgcct
      241 ttcttcactt ccttgccctg cgtgaagttg atggaggcga tgtagccgga gacgcgcggc
      301 ctgagctcga cgcgatccac ggcctcgaag cggccggtga actcatcgaa ctcggtcacc
      361 ggcttcgtga ggacctgggc caccgtcact tgcggagggg gcatgttggc cggggtcgct
      421 gcctcattac gggcgcaggc cgagagagtc agtgccacga tggcggtgag agaaacgagt
      481 gcaagagaag atcgcatggg tactccctcg gatcagaatt caatgaagcg ctgaggacct
      541 aatcggcagc gggtcgattc aaagacgatc gaccaccccc tcagacgacg cgcgtcgagt
      601 cccgcgcacg ccgggaatag tggtgcgggt caaggaactt cggtattgat caggcggagg
      661 ggatggaaag cgcccgggga cgggggacgg ggtattcctt ttgaggcagg ggggactttt
      721 gcaccagcga tccgctggcc gtccgcttca gtcggagcga ccgtctcctg gcagccctcc
      781 ttcggatgcg ccggcaagcc tctcagggtg cctcctcccg gagcttcatt tctttgttcg
      841 cagcgcgtcg ctgtcggggc cgactttgac ggtcaacgcg gagggtctgc gtcctcgtac
      901 ccgcaacacc tcactcccga gggcaaactc atcaccgggg gcgagccgcg tcggtgctgc
      961 gatgcgtgtc ccgttaactc aggtcccgtt cgtgccgtcg aggtcctcga tcgtagccgt
     1021 tccatgagcg atcgtggttc gtgcatgacg gcgcgagacc gtggtgcgtc gacaaccagc
     1081 gcacagtccg cgtctcgtcc accacatgct ccccgtccgc gagcagcaac gacggcctca
     1141 tccagagcac ctcgatcgcc gccggcgcgg gccgcgatac ggggcgaggc tggtgatatc
     1201 gtcgcagagc gcacagccag tcttgtaagc ggtgcgaatg atgcgggaat cgtgcggcgt
     1261 atcgccgagc accgcccgga gctccgagac cgggcgtgtc aggctggtgc gcgccccata
     1321 cacgcttggc cagagtagct cgtcgacgat gttggtgatg actgacggtc ggcgccggat
     1381 cagcacctcg agcaacagat acatcttcgg ctcgagagag accccttcat tgccgcgctc
     1441 gagctgccga gcgccgaggt cgaaatacga agccggcgaa cttcagttct cgtggtgccg
     1501 agtttcagga agctaccgct cgtcggctac cctttatctc tgcgcggatg gatgccggat
     1561 ctctgcagtg gcggccgatc ttttcaactg cgccggatct ccacgctgcc tgcggattcg
     1621 taccgggtct cccgatctcc atgttgccgg agtttctacg cagcgtcgct aaacggcgct
     1681 ccgatcatgt gtcgtatagc aggtcgccag tctggaaaca tcacgcgcat cgagcaggtg
     1741 tcgaagggag ggggtccgga cctggccctt gcccggaacc ccccggccac ttcatcgact
     1801 cgcggtgacc gtcggcggaa ggatgccgct cttcttctga tagaaggcgg tcagcaacgg
     1861 gacattcacc ttgctgatcg cctcactctc ggctttggtc atgcacgggc ggatcccgct
     1921 ctcctgcccg aagggctgat cgcagacgcg ctgcgcggcc gcatgaatgc gcctgtagag
     1981 cgcctccaca ccggccgggg tgtcgaggtt cagatcggca aacttcacgg tttcggaacg
     2041 aactccatcg tccgcattcg cattgaaagc gaccagcgtg cacgccagcc acatcgcggc
     2101 tgcgtaaccg aggatgcggc gcttatcgga cttgacattc gagctcatgg tggtctcctg
     2161 tacttgggtt tcggaacttc acttcgcctg ggacccgggt tgaaccaaca gtcctcaagc
     2221 ctcggatggt gaagttaccg acggcccggc cgcagatcct tatggagatc ttaggaaatg
     2281 gtgaatcttc ccccctcacg ggatgatgcg ccgcgtaagg gcccgacggg cggggcgcat
     2341 ttgctgtgat caggcaagga ggcgcatgct tttccaaacc gacagggcgc gcagcgcaga
     2401 gccgatgacc tcagaggaag accgcctccg cgcagcccgc tccacggaaa tcgcctcgcg
     2461 tgggaacagc cctcggctgg cagtcggtca gctgctcggc ggtcgctacc ggctcgagcg
     2521 cgagctcggt gagggcggca tgggggtggt ttatctggcg gccgatgagc aggtgccggg
     2581 cgagaagttc gccatcaagg tattgaagga ggagctgcac cccgaagccc tcgccctgct
     2641 gcgcgaggag gtccgcaaaa cgcgcaagct gagccatccc aacatcgtcg acgtgcattc
     2701 ggtgaacgtg gaaggccgca ggcttttcgt gttgatggag tatctcgagg gcaagtcgct
     2761 ggacatgctg ctcgatgagg agtttggccg cggcatgccc ttcagtcacg cctggccgat
     2821 catcaaggat gtgggtgcgg cactgggcaa cgcacacgac cacagcgtga tccacagtga
     2881 cttgaagcct gcgaatgttt tgctcacgac ctcgggaagg accaagctgc ttgacttcgg
     2941 catagcgcgg gtgacgcgcg ggccactgct acatgcccgc tccgggacgc gcgcactcac
     3001 cccggcctac gccagctgcg agatgctcga ggggagggag gcggaccgcc gcgatgacat
     3061 ttactccttc gcctgcgtga tctacgagat gctccgcggc gagcggccct tcgggcaact
     3121 caccgcgctc gaggcgcgaa acgcttctgc cccggtcccg ccgcttcagg ggatgtcgcg
     3181 cgagcagaat gcagcgctcc gccaagcgct ggccttcgac cgggaggcgc gcaccccatc
     3241 ggtggagcag cttctcgcgg ggctcaccgg cagccagcct cgcgctcgcg ctaacgcact
     3301 tctgacaggg gccatcttcg cggccatagc ggctttggga atcatcttct gggtcgctgc
     3361 ccggctatgg atccctcggc cttcgatcgt ggcagcgccg gtgacgtccc ccgaggctga
     3421 acgagcggcg gtctccccca aatccatcgc cgtgctgccg ctgctggatc tcagcacggg
     3481 acacgagcag gagtattttg ccgagggcct cacggccgct ctgatcgatc gattgacgct
     3541 gttccccggg ctgtcagtga ccggacagac ctcgagtttc gccttgaagg acagcaagga
     3601 agacgcgcct acgctcgcgc ggcggctgcg ggttgaatat ctcctcgaag gcacggtgcg
     3661 ggggtccgcc gagcagctgc gcgtcagcgc ccgcctgatc cgcgcagtgg acggcttcga
     3721 ggtgtggtcg caggtctttg accgccgcgg cagcgagatc ctcaagctgg aagaggacat
     3781 cgcgcgcgcc gtcgccgcag accttcgcgg gagccttctc gacaccccgg ttcccgtcgt
     3841 gccgacgacg cgctcgctcg aagcctaccg tctgctgctg cgggcggacc agctgtggcg
     3901 gcaggaattc gccacggacg acgagcgtgc gctgatcgaa gccgctctcc agaaggaccc
     3961 cggctacgca gccgcgtggg tcgaattggg attctggtgg tacgactcga tgctggtggg
     4021 taagacggag acgcaaacgg ccgtccccag agcgcgcggg gcgttcgacc gggcgctcgc
     4081 cctcgataag aactccagtc gcggctacat cggcctcgca tggctgcaga tgactcagca
     4141 tgactgggtg ggcgcgcagc gcagcatcga gcgtgcagac gccctcgatc ctgcaagtct
     4201 gtcggtgctg accgcgcacg gcgagttggc ccgcagcgcg ggccggtggg cggaggccat
     4261 ccgtctggag caagcgcgcg agcagcgaga tcctctcagt gtcggcacga agttcgtcct
     4321 cgcacaaagt catctgtgcg gaggcgagta cgcgaatgcc gaggacatcc tcagacaaac
     4381 gatcgcggag aaccccagat atcccggggc gcacgctctg ctcgcccgcg ctctcgcggc
     4441 gcggggtgag gccgctgccg cactcagggt cgtggccctg gagcccgacg agccggcgcg
     4501 tcttgctggc cttgcaagcg tccagtctga tctgggccga cccgatgcat ctgacgaggc
     4561 gatcactcag ctacgcacac gatacagcca gacattgccg ggactaaccg gactcgccta
     4621 cgcctatcgc aaccagctcg agctcgcttt cccatggctg cagcgagccg tcgagcagcg
     4681 ccagcccgag tttgcggcga gcctcgcctg cacgccggag cccgccttcg cttccctgca
     4741 cgctgatccg aggtttgctg ccctgagagg cggcctcgga ttcaagcggt gatgaagtcc
     4801 agtcctcagc ggttggacac cgatggcgta aggatctaga ctcgagccac gaagtcctgg
     4861 cgcacccgaa aaagaggcct cgccatgaaa aagaaggaca atggcttgga tgagctgctg
     4921 gcactcctca gggcaaaccc ggagctcatc aaagagctga tcttcgatcc agcgaacgtc
     4981 aagcgtctgc tcaagagcga ggcggcgcgc gagctcgtca tcggccagga cacggaggca
     5041 ttccttcggt atatcgccgg ccccgatgac ggctacccga ttgcccagtg cctgaagcaa
     5101 accaaatacc tgtgcgccaa ggggacgcgc cacgtggcct gcggcggagg caccaagtca
     5161 tcaaaatgag gtcccgcaag atccgccgcg gccgagcggt cctgcgaatt taacaggcgt
     5221 tcgatcatgc ccccacgcgt cagccg
//
LOCUS       DASRHN010000282        13206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_921780, whole genome shotgun sequence.
ACCESSION   DASRHN010000282 DASRHN010000000
VERSION     DASRHN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921780"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFB37_11355"
     CDS             complement(<1..345)
                     /locus_tag="VFB37_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZO23088.1"
                     /translation="MPDFDAHERVVFGYDEPTGLRAIIALHSTRLGPAAGGCRMWPYA
                     SSSEALTDVLRLSRGMSYKNAMAGVHFGGGKAVIIGDSRTMKTPELFQAYGRLVDSLG
                     GRYVTAEDVGTTT"
     gene            509..1000
                     /locus_tag="VFB37_11360"
     CDS             509..1000
                     /locus_tag="VFB37_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010207358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO23089.1"
                     /translation="MQAELDRGDVRILDVLQQHGDLSAADVAERVGMTASTCWRRVTR
                     LQELGVIKGRVALLDREKIGLNVMVFSHVKLAGHGRDALLRFEQAVREHPEILDCYTL
                     MGETDFLLRIVCRDIKAYEAFFLDHLSRFPGVQSVNSSIALAVIKESTALPLASMASV
                     GSR"
     gene            complement(997..1593)
                     /gene="rsmG"
                     /locus_tag="VFB37_11365"
     CDS             complement(997..1593)
                     /gene="rsmG"
                     /locus_tag="VFB37_11365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZO23090.1"
                     /translation="MDVALAERDAGRLLQLLDEVERWNRTYNLTAIRGREEMLTHHLL
                     DSLSVHKDLRGVRIADAGTGAGFPGLPLAVANPDRHFTLIDSNGKKIRFVQHAARLLG
                     LTNVEVLHARVQAVKVAEPFDSVLARAFASLRELVQAAGPLCAPQGRVLAMKGKWPEN
                     ELEALPAGWQAGGSHTLTVPGLEASRCVVLLERAEGHI"
     gene            1679..2341
                     /locus_tag="VFB37_11370"
     CDS             1679..2341
                     /locus_tag="VFB37_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO23091.1"
                     /translation="MRLLLVEDDSMIGEAIRTGLRRDGFTVDWVHDGDAAEEVLRTQP
                     FDLLLLDLGLPRKGGLQVLQRLRARHETLPVLIITARDAVSDRVQGLDAGADDYLVKP
                     FDLDELAARIRALLRRKSGRTAPQVEHLGVMLDPATHRVTREGRDIALSPREFALLQL
                     LLERPGNILSRAQIEERLYGWGEEVESNAVEVHIHALRRKLGADFIVTVRGVGYRVRP
                     AA"
     gene            2338..3681
                     /locus_tag="VFB37_11375"
     CDS             2338..3681
                     /locus_tag="VFB37_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO23092.1"
                     /translation="MTSLRTRLLVWLLGGVVLLGAAGAWIVYRNALAEADAFFDYHLR
                     ETALLLRDQPVQYMLGPAIPPGDADYDFVVQVWSLDGILVYLSRPHAVLPAITTLGFS
                     TASTSEGRWRVYGVQAVTRVIQVAQPMRVRERQAADLALRTLVPFALLMPLLAILIWF
                     AVGHALRPLQRLTQLVKTRKETALDPLPDARLPTEVQPLVSALNDLLQRLGAVLERER
                     GFMADAAHELRTPLTALHLQMSTLARAGTEAERADAMARLSAGVQRAIRLVEQLLALA
                     QQEPRVGSERAKLRLDELARDVVAEMVPVADARHIDLGICASEPATVLGDAQALRTLI
                     RNLVDNAVRYTPEGGTVDVSAMASAPSQPIHAPGGVGQAGSLLRVVDSGPGIAEQERS
                     RVFDRFYRQPGTSPPGSGLGLAIVKSIADAHGASVALNSGLAGRGLAVTVSFPPG"
     gene            3892..5379
                     /locus_tag="VFB37_11380"
     CDS             3892..5379
                     /locus_tag="VFB37_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZO23093.1"
                     /translation="MTSRNFFKGQIAAALLGAAIVAAPAGALYAFGAQAAPPVSRPAP
                     TVPAPATGNPNAVLPDFSTMVQKYGPAVVNITVVEKVPTAYSQGGSDDNDSGPSDQNP
                     FGGFPFGPFFRGFPAQPPQQVRGEGSGFIVSPDGTILTNAHVVNGASEVTVRLTDRRE
                     YTAKVIGVDTKSDVAVIKIPAKNLPTVKLGDDRGLKVGEWVVAIGAPFGFDNSVTAGI
                     VSAKGRTLPDSGYVPFIQTDVPINPGNSGGPLFNMQGEVVGINSQIYTRSGGYQGVSF
                     SIPIDVAMHVSQQLETHGHVTRAKLGVVIQQVDQGLADSFGLPQPEGALVSSVEKGGA
                     AEQAGIQPGDVILKLNGQPVDSSNELPATIASLQPGSTVKLEIWRQHATRDLNVKLGE
                     MQDKRTASNTDQSHSGGKIGLAVRPLTSEEQHEANTHGGLLIEQAQGPAADAGLQQGD
                     IVLAANGARVSSVADLRNAIEKSKSHIALLVQRGDTQLFVPVRVG"
     gene            complement(5494..6951)
                     /locus_tag="VFB37_11385"
     CDS             complement(5494..6951)
                     /locus_tag="VFB37_11385"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HZO23094.1"
                     /translation="MSSHDSRSARRPDPDAVLTAIADYARTHEVKSRQAYETAQFCLM
                     DTLACGFQALKYPACTKLLGPVVPGAVMPGGARVPGTSFELDPVQAAFNIGTMVRWLD
                     FNDTWLAAEWGHPSDNLGAILAVADYQARKAVMEGKGPPTVRDVLTAMIKAHEIQGVL
                     ALENSFNRVGLDHVILVRVASTAVATAMLGGSREQVINAVSNAWIDGGALRTYRHAPN
                     TGSRKSWAAGDATSRAVRHAFIALSGEMGYPSALSAPTWGFYDVLFKGKPFSLPQPFG
                     SYVMENVLFKISFPAEFHAQTAVEAAMTLHPEVGGRLEQIDRVVIETQEPGVRIIDKT
                     GPLANPADRDHCIQYMVAVPLIFGRLGAEDYEDQVASDPRIDALRAKMQVRENTRFTE
                     EYYAADKRYIGNAVQVFFRDGSCSARVQVDFPIGHRKRRAEGMPVLVKKFESCVDAWF
                     AAKQAERVKALFRDAALDRLPVNELVAALVTNGAA"
     gene            complement(6964..8124)
                     /gene="prpC"
                     /locus_tag="VFB37_11390"
     CDS             complement(6964..8124)
                     /gene="prpC"
                     /locus_tag="VFB37_11390"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HZO23095.1"
                     /translation="MSEAQSNIGGPKPKKSVALSGVTAGNTALCTVGVTGNDLHYRGY
                     DILEIAATCEFEEIAYLLVHGKLPNQAELAGYKAKLKSLRGIPAALREALESLPAASH
                     PMDVLRTGASVLGCILPEKDNHAEAGARDIADQLIASFGSMLTYWYHYSHNGQRIHVE
                     TEDDSVGGHFLHLLHRRQPSASWVRAMHTSLILYAEHEFNASTFAARVIAGTGADMHS
                     CITGAIGALRGPKHGGANEAAFEIQSRYAGPEEADADVRARVANREVIIGFGHPVYTI
                     SDPRNEVIKEVAHRLAKEAGDLKMFEVAERIESVMRETKKMFANLDWFSAVSYHLMGV
                     PTAMFTPLFVVARTSGWSAHVMEQRADGKIIRPSANYVGPENVKFVPIDKRK"
     gene            complement(8121..9017)
                     /gene="prpB"
                     /locus_tag="VFB37_11395"
     CDS             complement(8121..9017)
                     /gene="prpB"
                     /locus_tag="VFB37_11395"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HZO23096.1"
                     /translation="MANAASAGARLHAAVQAEHPLQIVGAITAYAARMAQATGFRALY
                     LSGGGVAANSLGMPDLGISTMDDVLIDIRRITEASSLPLLVDADTGWGSAFNIARTVR
                     SFIRAGAAGLHIEDQVAAKRCGHRPGKEIVALQEMVDRIKAAVDARTDDSFVIMARTD
                     ALAVEGLERTLERARACVAAGADMIFPEAMTDISMYRRFREATQVPVLANITEFGHTP
                     LYTRDELGAAGVDIVLYCCSAYRAMNAAALKTYQTIRRDGTQKNILGSMQTRDELYSY
                     LDYHEYEDRLNRLFASSKESQT"
     gene            complement(9153..10148)
                     /locus_tag="VFB37_11400"
     CDS             complement(9153..10148)
                     /locus_tag="VFB37_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZO23097.1"
                     /translation="MTSFKALRIHEDGGRIRARFEQLSLEDLSPGEVVVRVTHSSINY
                     KDALAATGAGKILRRYPLVGGIDLAGVVETSVDPRYSPGEAVLVTGSSLSETHDGGYA
                     EYARLKGDWVIPMPPGLDAETAMALGTAGFTAALAIHRMERNDQQPSRGPVAVTGATG
                     GVGSLAVDMLASRGYEVVAVTGKASRAEYLKALGAKVLLARQDIDFGSRPLEGIRFGG
                     AIDSVGGEMLTWLTRTVDFWGNIASIGLAGSAELKTTVMPFILRGVNLLGINSSATPR
                     PLRLEVWKRIATDLKPRHLDRIRTQTIAFDDLPAAFPAYLDGSVTGRTVVRIGPG"
     gene            complement(10165..11139)
                     /locus_tag="VFB37_11405"
     CDS             complement(10165..11139)
                     /locus_tag="VFB37_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO23098.1"
                     /translation="MNLSYTPQVIRHPHGIFAVDAEYLYPGHAAVHIIRERGRAAFVD
                     TGTSHSVPYLLAALDALNISRDAVDYVFLTHVHLDHAGGAGRLMRELPNARAVVHPRG
                     EPHMIDPAKVIAGSAAVYGEERFRQLYGDILPIEPERLITAPDGYRCELAGRKFELLH
                     TPGHALHHYAIVDRAHSLIFAGDTFGISYRALDTQQGAFILPTTSPSQFDPQQLTASV
                     DRLLELAPESIYLMHYSRVTGIPRLGAMLKEQIQELVRITRELAGSQDPHAAIREAML
                     ELWWTLLQRQGSRLSRSEVAHQLESDLELNTQGLLIWLHRAERSRPAG"
     gene            11199..13157
                     /locus_tag="VFB37_11410"
     CDS             11199..13157
                     /locus_tag="VFB37_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO23099.1"
                     /translation="MTIRHASRRLLLGPIALLAALPVTAQSPAPSHRERGNLILEGIP
                     ASDATLLEPLARYRQSRQATFLDWLPDGAMLVATRFGDVEQVHRVASPLGMREQLTFY
                     SEPVSTARAPQTGSGDGFVFLKDQGGDENDQLFYYAPGGHVQQLTSGKFVHGSPIWSH
                     DGKRVAFYGNERNGIGYDIYVVDIGSTAAPRLVAGGQQDSWFPLDWSPDDRKLLLSKF
                     VSINESYLYIADVYTGTLTPVDESGHKNGIRVARFAPDGRGIYLSSDEDGEFAQLRYL
                     DPVSHEVRRLTGNIPWDVEEFDVSGDGRYVAWVANEDGRNRLTITDTVQKLEQAPAGI
                     PDGRITGLRFDRTGRRLAFSAESAQAPRDVYVYDLSHSALERWTRSEVGPVDVSTFVP
                     AELVRYPTWDRMNGGQRMISAYMYRPRSAGPHPVLIDIHGGPESQFRPTWNPFIQFVV
                     NELGYAVIAPNVRGSSGYGKTFLKLDNGLLREDAVKDIGSLIVWIDLQPMFDREHVAV
                     MGGSYGGYMALASLASYGDRLKGGIDEVGISNFVTFLENTSPYRRELRRAEYGDERDP
                     KMRAFLNRISPLNNVASIRRPLLVVQGLNDPRVPPAESQQIVWRLRGRGGEVWYLAAK
                     DEGHGFRKKANQDVLQETVAMFLQKLAH"
BASE COUNT         2348 a         4245 c         4258 g         2355 t
ORIGIN      
        1 ggtggtcgtg cccacgtctt ccgcagtgac gtagcggcca ccgagcgagt ccaccagtcg
       61 accgtaagcc tggaacaact ccggtgtctt catcgtgcgg gaatcgccga tgatcaccgc
      121 cttgccgccg ccgaaatgca caccggccat cgcattcttg tagctcatgc cacgcgagag
      181 gcgcagcaca tccgtgagcg cttcgctgga ggaggcgtag ggccacatgc ggcaaccgcc
      241 ggccgccggt cccaggcgcg tcgaatgcag ggcaatgatc gctcgcagtc cggtcggctc
      301 gtcgtacccg aacacgactc gctcgtgagc atcaaaatcc ggcatgccgt acagatccat
      361 caccgtgtcc cccgcaaggg ctcccagaag gcgcctttcc gagcggaggg tactcgccgg
      421 ccagcgcacg ctgcgtcaca aaagttgctc tcaagcccgc actggtgaag tatccatagc
      481 accgtgaggc ctcgaagtga gatgagctat gcaagcggag ctcgaccgtg gggacgtgcg
      541 gattctggat gtcctgcagc agcacgggga tctgtcggcc gccgacgtgg cggagcgggt
      601 cggcatgacc gcctcgacct gctggcgccg tgtgacgcgt ctgcaggagc tcggcgtcat
      661 caagggccgc gtcgccctgc tggatcggga aaagatcggc ctgaacgtca tggtgttctc
      721 acacgtgaag ctcgccgggc acggccggga tgccctgctg aggttcgagc aggccgtgcg
      781 cgagcacccg gagatcctgg attgctacac gctgatgggc gagacggact tcctgctgcg
      841 catcgtgtgc cgcgacatca aagcctacga ggctttcttc ctcgaccatc tatcgcggtt
      901 ccccggcgtg cagtcggtga attcctcgat cgcccttgcc gtcatcaagg agagtactgc
      961 gctgccgctc gcatcgatgg ccagcgtcgg ctcgcgctag atgtggccct cggcacgttc
     1021 gagcaatacg acacagcgcg acgcttccag ccctgggacc gtcagagtgt gagagcctcc
     1081 ggcttgccag cccgcgggca acgcctcgag ctcgttctcg ggccatttgc ctttcatggc
     1141 aagtacccgc ccctgcgggg cgcacagggg acctgccgct tgcaccagct cccgcaggga
     1201 agcaaacgct cgcgccagaa cggagtcgaa cggctcagcc accttcacgg cctgcacgcg
     1261 cgcgtgcaac acctcgacat tggtcagacc caacaggcgt gcggcatgct ggacgaagcg
     1321 gatcttcttg ccgttcgagt cgatgagcgt gaaatgccgg tccggattgg ccaccgcaag
     1381 cggcaaccca gggaagccgg cgcccgtacc ggcatcggcg atgcgcacac cccgcagatc
     1441 tttgtgcacc gacaggctgt ccaggagatg gtgcgtcaac atctcctcac gaccgcggat
     1501 cgcggtcagg ttgtaggttc gattccagcg ctcgacttca tccagcagct gcagcagccg
     1561 tccggcatcg cgctccgcga gcgcaacatc catcgtggct gcatcttgga ccagacgttg
     1621 aatcgtgctc gacaaggggg ccgccggatg agtcgcagcc gctaggataa ggccgaccat
     1681 gagactgctg ctcgttgaag atgattcgat gatcggcgag gccatccgta cgggcctgcg
     1741 gcgggatggc ttcacggtgg actgggtgca tgacggagat gcggcggagg aggttttgcg
     1801 cacccagccg ttcgacctgc tgctgctgga tctgggcctg ccgcgcaagg gcggcctgca
     1861 ggtgttgcag cgcctgaggg cacggcacga gacactgccg gtgctcatca ttacggcccg
     1921 cgatgcagtt tccgatcgcg tccaagggct ggatgcggga gcggatgact atctcgtcaa
     1981 gccgttcgat ctggatgagc tcgcggcacg tattcgggcc ctgctgcgtc gcaagtcggg
     2041 caggaccgct ccccaggtgg agcacctcgg cgtgatgttg gatccggcta ctcaccgcgt
     2101 gacccgcgag ggccgtgata tcgcattgtc gccgcgagag tttgcgctcc tgcaactgct
     2161 gctggaacgg cccggcaata ttctttcacg cgcgcagatc gaggagcgcc tttatggctg
     2221 gggcgaggaa gtcgaaagca acgcagtcga ggtgcacatc cacgcgctga gacgcaagct
     2281 cggagcggac ttcatcgtga ccgtccgtgg agtgggctac cgcgtgaggc cggcggcgtg
     2341 acatcgcttc ggacgcggct tctggtttgg ctgctcggcg gcgtcgtact gctaggggcg
     2401 gcgggtgcat ggatcgtcta ccgcaacgcc ctcgcagaag cggacgcctt cttcgattat
     2461 cacttgcgag agacggccct gctgcttcgc gatcagccgg tgcagtacat gttgggaccg
     2521 gccattccgc cgggggacgc ggactatgac ttcgtcgtac aggtgtggtc gctggacggc
     2581 attctcgtgt atctgtcgag accgcacgcc gtgttgccgg ccatcacgac actcggcttc
     2641 tctacagcct ccaccagcga aggccgctgg cgtgtctacg gcgtacaggc ggttacgcgg
     2701 gtcatccagg tggcccaacc gatgcgagta cgcgagcggc aagccgcgga ccttgcgctg
     2761 cgaaccctgg tcccgttcgc cttgttgatg ccgctgctgg cgatcctcat ctggttcgcc
     2821 gtagggcatg ccctcaggcc gctgcagcgc ctcacacagc tcgtaaagac ccggaaagag
     2881 accgcgcttg atccactgcc tgatgcgaga ctgcccaccg aggtgcaacc gctcgtcagt
     2941 gcgttgaacg atctgctgca acggctgggc gcggtgctgg aacgcgagcg agggttcatg
     3001 gccgatgcgg cccacgagtt gcgaaccccg ctgacggctc tgcatctgca gatgagcacg
     3061 cttgcccgcg cgggcaccga ggccgagcga gccgacgcca tggctcgact ctccgcgggt
     3121 gtgcaacgtg cgatccgtct ggtggagcag cttctagcac tggcacagca ggagccccgg
     3181 gtggggtccg agcgagcgaa gcttcgattg gacgagcttg cacgtgatgt cgttgcggag
     3241 atggtgccgg tggcggatgc ccggcatatt gatctcggga tctgcgcatc ggaacccgcg
     3301 acggtactgg gcgatgccca ggccctgcgc actctgatcc gcaacctcgt cgacaatgca
     3361 gtgcgttaca cgccggaagg tggcacggtc gacgtctcgg caatggcgag cgcgccatct
     3421 cagccgatcc acgcccccgg cggcgtcggg caggcaggca gtctccttcg cgtcgtggat
     3481 tccggtccgg gtatcgccga gcaggagcgc tcgcgtgtct tcgatcgctt ttatcggcag
     3541 cctggcactt cgccaccggg cagcggcctg gggctggcca tagtcaagtc gatcgcggac
     3601 gcacacgggg cgagtgtggc gctcaactcc ggcctggcag ggcgcggctt ggcggtgacg
     3661 gtcagtttcc cgccgggctg agcgtatcgc ggtgaatggg aagcgtcatc tcggcaaaca
     3721 tgcacggctt caacattacg ccacccgtct cggccggccg ccgccgcgat acgctcgcaa
     3781 aatcaatggt ctattacggc cagggcccgg gttgaggccc ttaagggtcc cttaaggcgg
     3841 agggtttagg gtacgtcaca tggatttggt gccactccct cggagactcc catgacttcc
     3901 cggaatttct tcaaaggtca gatcgccgcg gcgctgctgg gcgccgcaat agtcgctgcg
     3961 cccgcagggg ctctttacgc attcggcgct caggctgccc cacccgtctc acgcccggcg
     4021 cccaccgtgc cggctcccgc gacgggcaat cccaacgcag ttcttccgga cttcagcacg
     4081 atggtgcaga agtacggtcc ggccgtagtc aacatcacgg tcgtggaaaa agtgccgact
     4141 gcctactcgc aaggcggctc ggacgacaac gattccggtc cctcggatca gaaccccttc
     4201 ggcggattcc cgttcggacc cttcttccgc ggctttcccg cgcagcctcc gcagcaggtg
     4261 cgcggtgagg gctcaggttt catcgtgagt cccgacggca cgatcctgac gaacgcgcat
     4321 gtggtcaatg gcgccagcga agtcaccgtg cgcctgaccg accggcgcga gtacaccgcg
     4381 aaggtgattg gcgtcgacac gaagagcgac gtggcggtca tcaagattcc ggccaagaat
     4441 cttccgacgg tcaagctggg cgatgatcgc ggtctgaagg tcggcgaatg ggtcgtcgcc
     4501 atcggcgcac cctttggatt cgacaacagc gtgaccgcgg gcatcgtgag cgccaaggga
     4561 cggacgctgc cggactccgg ctacgtgcca ttcatccaga ccgacgtgcc gatcaatccc
     4621 ggtaactcgg gtggccccct gttcaacatg cagggcgagg tggtcggcat caactcgcag
     4681 atctacaccc gctcgggcgg ttaccagggc gtgtcgttct cgatcccgat cgatgtggcc
     4741 atgcacgtga gccagcagct cgagacccat ggacatgtca cgcgcgccaa gctgggtgtg
     4801 gtgatccagc aggtcgacca gggcctggcg gattccttcg gtctgccgca gcctgaaggc
     4861 gccctggttt cgagcgtgga gaagggcgga gcggccgagc aggcgggtat ccagccgggc
     4921 gatgtcatcc tcaagctgaa cggtcagccc gttgactcct cgaacgagct gccggcgacg
     4981 atcgccagcc ttcagcccgg gagcacggtg aaactcgaga tctggaggca gcatgccacg
     5041 cgtgacctga acgtcaagct cggtgagatg caggacaagc gtacggcatc caacaccgac
     5101 cagtcgcact ccggcggcaa gatcgggctc gccgtgcggc cgctgaccag cgaggaacag
     5161 cacgaggcca acacccatgg cggtctgctg atcgagcagg cgcagggacc ggcagccgat
     5221 gccggcctcc agcagggtga catcgtgctg gccgcgaacg gtgcccgggt gtcgagcgtg
     5281 gcggacctcc gcaacgccat cgagaagtcc aagagccata tcgctctgct cgtgcagcgt
     5341 ggcgataccc agctcttcgt accggtccgc gtgggctgaa gtccctccga atatcgcgaa
     5401 tcggaaaacc ggcagcgcaa gctgccggtt ttctttcttt atgggctgcg aaaagcctgt
     5461 cggttcgaac gcgaaccggg acggactcga ctctcaggcc gcgccgttgg tcacgagtgc
     5521 cgcgacgagc tcgttgacgg gcagccggtc gagcgccgca tcccggaaca aggccttgac
     5581 ccgctctgcc tgcttcgcgg caaaccaggc gtcgacgcag gactcgaatt tcttcacgag
     5641 caccggcatg ccctcggcgc ggcgcttgcg gtggccgatc gggaaatcga cctggacacg
     5701 cgcgctgcag ctgccatcgc ggaaaaacac ctggacggcg ttgccgatgt aacgcttgtc
     5761 agctgcgtaa tactcctccg tgaaacgggt attctcgcga acctgcatct tcgcgcgcag
     5821 cgcatcgatt cgcggatcgg atgcgacctg atcctcataa tcctcggcgc cgagccggcc
     5881 gaagatcagc ggcaccgcga ccatgtactg aatgcagtgg tcgcggtccg caggattggc
     5941 cagcggaccg gtcttgtcga tgatgcgaac gcccggctcc tgcgtctcga tgaccacccg
     6001 gtcgatctgc tcgagacggc cgcccacttc gggatgcagc gtcatggcgg cctcgaccgc
     6061 cgtctgtgcg tgaaactccg ccggaaagga aatcttgaac agcacgttct ccatcacgta
     6121 gctgccaaag ggctgcggca acgagaaggg cttgcccttg aacaggacat cgtaaaagcc
     6181 ccaggtcggc gcactcaatg cggaggggta gcccatctcg ccgctcagcg cgatgaatgc
     6241 atgccgcacg gcacggctgg tcgcatcccc cgcggcccag ctcttgcgcg agccggtgtt
     6301 cggcgcgtgg cgataggtgc ggagtgctcc cccatcgatc caggcattcg acacggcatt
     6361 gatcacctgc tcgcgcgagc cacctaacat tgcggtcgcg accgcggtcg atgcgacgcg
     6421 aaccaggatc acatgatcca gtccgacgcg gttgaagctg ttctccaggg cgagcacacc
     6481 ctggatctcg tgcgccttga tcatggcggt cagcacatcg cgcaccgttg gcggcccctt
     6541 gccctccatc acggccttgc gcgcctgata gtccgccacg gccaggatcg cgccgaggtt
     6601 gtccgaagga tggccccact cggcggcgag ccaggtatcg ttgaaatcga gccaacgaac
     6661 catcgtgccg atgttgaaag cggcctggac gggatcgagc tcgaaagaag tgcccggtac
     6721 ccgtgcgccg cctggcatca cggcccctgg cacgaccgga cccagcagct tcgtacaggc
     6781 cggatatttc agcgcctgga aaccgcaggc cagcgtgtcc atgaggcaga attgcgccgt
     6841 ctcgtaggcc tgccggctct tgacctcatg ggttctcgcg tagtcggcga ttgcggtgag
     6901 caccgcatcc ggatcagggc gccgggcgga tctggaatcg tgagaggaca tgctggcgag
     6961 ccgttacttt cttttgtcga tcggaacgaa cttgacgttc tccgggccca cgtagttggc
     7021 cgagggccgg atgatcttgc cgtcggcccg ctgttccatg acatgggccg accagccgga
     7081 ggtacgcgcc acgacgaaaa gcggggtgaa catcgcagtg ggcacaccca tcaggtgata
     7141 ggacacggcg gaaaaccagt cgaggttggc gaacatcttc ttcgtctcgc gcatcaccga
     7201 ctcgatgcgc tccgcgacct cgaacatctt caggtcgccg gcttccttgg ccaggcggtg
     7261 cgcgacttcc ttgatcacct cgttgcgcgg atcactgatc gtatagacgg gatggccgaa
     7321 gccgatgatg acttcacgat ttgcgacccg cgcccgcacg tctgcatcgg cctcctcggg
     7381 accggcgtaa cggctctgga tctcaaacgc tgcctcattc gcgccaccat gcttggggcc
     7441 gcgcaaggcg ccgatcgctc cggtgatgca cgagtgcatg tccgcaccag taccggcgat
     7501 gactcgggcg gcaaaggtgg aggcattgaa ctcatgctct gcgtagagga tcagcgaggt
     7561 atgcatcgcc cgcacccacg aggccgaagg ctgcctgcgg tgcaacaggt gcaggaagtg
     7621 cccgcccacc gaatcgtcct cggtctccac gtggatccgc tggccattgt gactgtagtg
     7681 ataccagtaa gtgagcatcg acccgaagga ggcgatcagc tggtcagcga tatcacgggc
     7741 gccggcctcg gcgtgattgt ccttttccgg caggatgcag ccaagtaccg aggcgcccgt
     7801 gcgcagcaca tccatggggt gggaggcggc gggcagcgac tcgagcgcct cgcgcagtgc
     7861 ggcgggaatg ccccgtaggg atttcagctt cgccttgtaa ccggccagct ccgcctggtt
     7921 tggcagcttg ccatgaacca gcagataggc gatctcctcg aactcacacg tggcggcgat
     7981 ctcgagaatg tcatagccgc ggtagtgaag gtcgtttccc gtcactccga ccgtacacaa
     8041 ggcggtattg cccgcggtca cgccggagag tgccacggac ttcttcggct tcgggccgcc
     8101 gatgttggat tgcgcctcgc tcacgtctgg ctctccttcg aggatgcgaa cagtcggttc
     8161 agcctgtctt cgtattcgtg atagtccaga taactgtaga gctcgtcgcg ggtctgcatg
     8221 ctgcccaaaa tgttcttctg cgtgccgtcg cggcgtatgg tctggtaggt cttcagcgcc
     8281 gcggcattca ttgcgcgata cgccgaacaa cagtacagca cgatgtccac gccagcggcc
     8341 ccgagctcgt cgcgcgtata gagcggcgta tggccgaatt ccgtgatgtt ggccaggacc
     8401 ggtacctggg tggcctcgcg aaagcgccgg tacatggaga tgtccgtcat cgcttcgggg
     8461 aagatcatgt ccgcgcccgc cgcgacgcac gcccgggcgc gttcgagcgt gcgctcgagg
     8521 ccctcgaccg ccagtgcatc ggtgcgcgcc atgatcacga aggaatcgtc ggtgcgcgca
     8581 tccacggccg ccttgatccg atcgaccatc tcctgcagcg caacgatttc cttgccggga
     8641 cgatgaccgc aacgcttggc cgcgacctga tcttcgatgt gcagacccgc tgcaccggcc
     8701 cggatgaagg agcgcacggt ccgtgcaatg ttgaatgcgc tgccccaacc ggtatcggca
     8761 tcgaccagca gcggcaagga gctcgcctcg gtgatgcgac ggatgtcgat gaggacatcg
     8821 tccatcgtcg agatgcccag atccggcatg ccgagtgagt tcgcggcgac tccaccccca
     8881 gagaggtaca gcgcgcgaaa gcctgtggcc tgcgccatgc gcgccgcata ggcggttatg
     8941 gcaccgacta tctgcagggg atgctcggcc tgtaccgccg catgcagacg cgcgccggcg
     9001 gatgctgcat tcgccatcgt agacctcgac tgggaccgat acattgtata cgccccgcgc
     9061 cttgccagga tggatttaac ccgccagtag actatctact cgcgaggcta gatcgccgcg
     9121 gcagcgcgcg cgcccccggc ccgagcccgg cgctacccgg gcccaatcct tacgacggtc
     9181 cgaccggtga cgctaccgtc gaggtaagcc ggaaacgcgg caggcaagtc gtcaaaggcg
     9241 atcgtctggg tgcggatgcg gtccaggtgc cggggcttca gatcggtcgc gatgcgcttc
     9301 cagacttcga gccgcagcgg ccgtggcgtg gccgaagaat tgatgccgag cagattcacg
     9361 ccgcgcagaa tgaacggcat cacggtggtc ttgagctcgg cgctgcctgc cagcccgatg
     9421 ctggcgatgt tgccccagaa gtccacggtg cgggtgagcc atgtcagcat ttccccgccg
     9481 acgctatcga tcgcaccgcc aaagcgaatg ccctccaggg gcctcgagcc aaagtcgatg
     9541 tcctggcgcg ccagaagaac tttggcgccc agcgccttca gatactcggc ccgggaggcc
     9601 ttgcccgtga ccgctacgac ctcatagcca cgcgaagcaa gcatatcgac tgcaaggctg
     9661 ccgacgcctc ccgtagcgcc cgtgacggcg accggcccgc gggaaggctg ctggtcattg
     9721 cgctccatgc gatggatggc cagcgcagcg gtgaaccctg ccgtgcccag cgccatcgcc
     9781 gtctcggcat cgagtccggg tggcattggg atcacccagt cacctttcag gcgggcgtat
     9841 tccgcgtaac cgccatcgtg cgtctccgag agagagctgc cggtgacgag caccgcctct
     9901 ccgggtgagt agcgcggatc gacggaagtc tcgaccacgc ccgccagatc gataccgccg
     9961 accagcgggt agcgccgcag aatctttccc gcacccgtcg cggccagagc gtccttgtag
    10021 ttgatgctgg aatgggtcac gcgcacgacc acctcgccgg gcgagaggtc ttccaggctc
    10081 agctgctcga accgagcgcg gatgcggccg ccgtcctcgt ggatccgcaa tgccttgaaa
    10141 gaagtcatac gatcttcccc gctctcagcc ggccggcctg ctgcgctcgg cccgatgcag
    10201 ccagatcagc aggccctgcg tgttcagctc gaggtcggac tcgagctgat gcgccacttc
    10261 ggaacgactc agccggctgc cctggcgctg cagcagcgtc caccacagct cgagcatcgc
    10321 ctcacgaatg gcggcgtgcg gatcctgcga tccggccagc tcgcgcgtga tccgcaccag
    10381 ctcctggatc tgctccttca gcattgcgcc caatcgcggg ataccggtca cgcggctgta
    10441 atgcatgagg tagatggact cgggtgcaag ctccagcaga cggtcgacgg aggcagtgag
    10501 ctgctgagga tcgaactgtg acggactcgt cgttggcagg atgaacgcgc cctgctgcgt
    10561 atcgagtgcg cggtaggaga tgccgaacgt atccccggca aagatcagcg agtgcgcccg
    10621 gtcgacgatg gcatagtgat gcagggcatg cccgggtgtg tgaagcagct cgaactttcg
    10681 gccggccagc tcgcaccgat agccgtcagg ggcggtgatc agtcgctcgg gctcgatggg
    10741 caggatgtct ccgtacagct gccgaaaacg ctcctcgccg tagaccgcgg cggagccggc
    10801 gatcaccttg gccggatcga tcatgtgcgg ttcgccgcgc ggatgaacca cggcacgcgc
    10861 attcggcagc tcgcgcatga gccgtcccgc accgccggca tgatcgaggt ggacgtgcgt
    10921 caggaagacg tagtccaccg catcgcgtga aatgttcagc gcatcgagtg ccgcaagcag
    10981 atacggaacc gaatggctgg tccccgtgtc cacgaacgcg gcacgacccc tctctcggat
    11041 gatgtggacg gctgcgtgac cgggatagag gtattccgcg tccaccgcaa agatgccatg
    11101 cggatgtcgg attacctgtg gcgtgtagct gaggttcatg gtcgagcgcg tttcgggcaa
    11161 catagcaaaa attgacggcc cttttgtaat cgacacgcat gactatccga cacgcatcgc
    11221 gaaggcttct gctcggcccg atcgctctgc tcgctgccct tccggtcacg gctcagtccc
    11281 ctgcgccctc gcatcgcgag cggggtaatc tgattctcga aggcatcccg gctagcgatg
    11341 ccacgctgct cgagccgctg gcacgctacc ggcaatcgcg ccaggccact tttctggatt
    11401 ggctgcccga tggggccatg ctcgtggcga cccgcttcgg cgatgtggag caggtgcacc
    11461 gggtcgcgag cccgctgggc atgcgcgagc agctcacttt ctacagcgag cccgtgagca
    11521 cggcccgcgc gccccagacc ggctcgggcg acggattcgt gttcctcaag gatcagggcg
    11581 gggatgagaa cgatcagctt ttctattacg cgcccggcgg gcacgtgcag cagctcacat
    11641 ccgggaaatt cgtccacggt agtcccatct ggtcgcatga cggtaagcgc gttgctttct
    11701 acggcaacga gcgcaacggc atcggctatg acatttatgt cgtggacatc ggctcgacgg
    11761 ccgctccccg gctggtggcc ggcggtcagc aggacagctg gtttcccctg gactggtcac
    11821 cggatgatcg caaactgctc ctctcgaagt tcgtctcgat caacgaaagc tatctctaca
    11881 tcgccgatgt ctacaccgga accctgacgc ccgtggacga gagcggccac aagaacggca
    11941 tccgtgtcgc ccgtttcgcc ccggacggtc gcgggatcta cctgtcaagt gatgaggatg
    12001 gagagttcgc gcaattgcgc tatctggatc cggtctcgca cgaggttcgc aggctgacgg
    12061 ggaacattcc gtgggatgta gaggagttcg atgtgagtgg cgacgggcgt tatgtggcct
    12121 gggtcgcaaa cgaggatggg cgcaatcgcc tgacgatcac cgacacggtc cagaagctcg
    12181 agcaggcccc cgccggaatc cctgacggtc gcatcaccgg cctgcgcttc gatcggaccg
    12241 ggcgtcgcct ggctttctcg gccgagtcag cgcaggcgcc tcgcgatgtt tacgtctacg
    12301 acctcagcca cagcgctctc gagcgctgga cgcgcagcga ggtaggaccc gttgacgtga
    12361 gcaccttcgt cccggcggag ctggtgcgct atcccacctg ggatcgtatg aacggcggac
    12421 agcggatgat ctcggcgtac atgtatcgac cgcgcagcgc cggaccccac cccgtactca
    12481 tcgacattca cggcggcccc gaatcccagt tccggcctac ctggaacccc ttcattcagt
    12541 tcgtcgtcaa cgagctgggc tatgccgtga tcgcacccaa cgtgcgcggc tcctccggtt
    12601 acggtaagac ctttctcaag ctcgacaacg ggctcctgcg cgaggatgcg gtcaaggaca
    12661 tcggctcgct gatcgtgtgg atcgacctgc agcccatgtt tgatcgcgag cacgtggcgg
    12721 tcatgggagg ctcgtacggc gggtatatgg cgctcgcttc tctggcctcc tatggcgatc
    12781 ggctcaaggg tgggatcgat gaggtaggca tcagcaactt cgtcaccttc ctcgagaaca
    12841 cctcgcccta ccggcgcgag ctgcgccgcg ccgaatacgg cgacgagcgc gatccgaaga
    12901 tgcgagcctt cctgaaccgc atctcgccgc tcaacaatgt cgcctccatc cgtcggccgc
    12961 tgctggtcgt gcagggcctc aacgacccgc gggtgccacc ggccgagtca cagcagatcg
    13021 tgtggcgcct gcggggccgc ggtggtgaag tgtggtatct ggcggccaag gacgaggggc
    13081 acggcttccg caagaaggcg aatcaggacg tcttgcagga aaccgtcgcg atgttcctgc
    13141 agaagctggc gcattagcca gcgagtcgga aagggcatgg aaggcgaaag gctcttcagt
    13201 gcgccg
//
LOCUS       DASRHN010000283         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_589985, whole genome shotgun sequence.
ACCESSION   DASRHN010000283 DASRHN010000000
VERSION     DASRHN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589985"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VFB37_11415"
     CDS             complement(<1..1180)
                     /locus_tag="VFB37_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZO23100.1"
                     /translation="MPFTSTMRDVPAARRRGWRRIALLTFAALWVGTAFWQTNKPLRP
                     GLHIDSAWYPVAPRDVMFIADVTSADAYGRPIVSQAIFDEVLNVVHSARRFIVLDYFL
                     FGAPAGAAAQAQPDPPPGVLPRPLSAELRGALIEQRRAHPELQVLFITDPISELYGDR
                     PSADLSRLRAAGVDVTVTDLKRLRDPNFIYSSLWRLGIGWWSGTGSAGSAPRRGWLPN
                     PILESDAPVSLRAWTRLLNFKANHRNVILADDGRDGLVAIVGSASPHDPSAAQSNAAL
                     KITGPAMLPLLRSELAVAQFSGWRGSLQPIAALAGASSTGPATTTGPRAKVLTEGAIR
                     SALIERLEACARGDSIDIATFYIADRGIVEALLAASRRGVTVRLILDPNKDAFGHARG
                     G"
     gene            complement(1263..>3758)
                     /locus_tag="VFB37_11420"
     CDS             complement(1263..>3758)
                     /locus_tag="VFB37_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO23101.1"
                     /translation="IFIVSAASLKLKYALAAFVVVLLVTILATAVLAWQDDVGTRRLG
                     IVAENSARDRVAVELRARAESTAAHAAEAVAGAVRAHDTESIAHRLQPFLEDRTVAEV
                     TVTDSDGATLYRWRRPTPSRATLQFEAAQPVREMVESIPGAATPKTFGQLRILVDQIA
                     PSPAMSLGRHLAAANSEQMQLALLIAGGLALGGGLLGAALAWRAGRKLEQPIVAMIRG
                     AERIGQGDYTRPVDVRRRDELGELQMALERMRGRLRQSTVHKNYLHSVLNSMTDAVFV
                     TSPDGVVKVANSAACKLLGYAEEEILGRSIIAMLDERERDSFDLLQATQETRETVVRT
                     RGGQTIPVSFTGSRIETDDPQFQGNIFVARNITDRKRAERRIRYLARYDALTKIPNRM
                     QFHHLLQQTIARGLRSGQVLALLYLDMDRFKEVNDTFGHASGDRLLEVLAERLTRLLP
                     KDTILGRLAGDEFALFVDGLPADADNRGPIAHLARSILTEVARAFQLNQHEVFLTASI
                     GIAFCPRDAENVIDLIRNADAAMYYSKQNGGNTFAFYSPEMNAAAVERLMLKSKLRRA
                     LERDELVIRYQPKVDLRNGRIIGAEALLRWRLPGHGDIPPGMFIPLAEETNLILDIGE
                     WVLNRVCLDYRRMQSEGASPGRISLNLSLKQLRQASFILRCRSVFRRHSVSPTSFELE
                     ITETTLMADAKRTVRLLDELYAMGLHLSIDDFGTGYSSLSALQQFPIGTLKIDQSFVR
                     NVSEDAGDATIVKTIIEMGRSLGMEVVAEGVESLRHLDFLRRNACHYAQGKLFGEPCT
                     AEDLQALLSRQVTGTAPFAQLMHHADELASRLA"
BASE COUNT          655 a         1196 c         1276 g          631 t
ORIGIN      
        1 ttccgccccg cgcgtgcccg aaggcgtcct tgttgggatc cagaatgagg cgcacggtca
       61 caccgcgccg gctcgccgcg agcagtgctt ccacgatgcc gcgatcggcg atgtagaaag
      121 tcgctatgtc gatgctgtcg ccgcgggcac aagcctccag tcgctcgatc agcgcggacc
      181 ggatggcgcc ttcggtgagc accttcgcgc gcgggccggt tgtggtagcg ggcccggtcg
      241 agcttgcgcc agccagggct gcaatcggct ggaggctgcc gcgccacccg gaaaactgtg
      301 cgacggccag ctcgctgcgc agcagcggta acatcgccgg gcccgtgatc ttcagggccg
      361 cgttcgattg agcggcgctg ggatcgtgcg ggctggcgga gccgacgatc gcgacaagtc
      421 cgtcacgccc gtcatccgcg agtatgacgt tgcggtggtt ggctttgaag ttgagaaggc
      481 gggtccaagc acgcaggctc accggtgcgt cgctttccag gatcgggttg ggtagccagc
      541 cgcggcgggg tgcgctgcct gcggagccgg tcccgctcca ccagccgatg ccgagtctcc
      601 acaggctgga gtagatgaag ttgggatcac gcaaccgctt gagatcggtg accgtcacat
      661 ccacgccggc agcccgcaga cgcgacaggt cggcggaggg ccggtcgccg tagagctcac
      721 tgatcggatc ggtgatgaac agaacctgca gctccgggtg cgcacggcgc tgctcgatca
      781 gcgcgccgcg cagctctgca gacagcggcc gtggcagcac gccgggcggg ggatcgggct
      841 gcgcttgcgc cgcggcccca gcgggggctc caaacagaaa gtaatccagc acgatgaagc
      901 gccgagcgga gtgcacgacg ttcagcactt cgtcgaagat ggcctggctg acgatcggtc
      961 tgccgtaggc atccgctgag gtgacatccg cgatgaacat gacatcgcgc ggcgccacgg
     1021 gataccaggc cgagtcgatg tgaaggccgg gcctgagcgg cttgttggtc tgccagaagg
     1081 cggtgccgac ccagagcgcg gcgaaggtga gcagggcgat gcgcctccac ccgcgccgac
     1141 gtgcagcggg cacgtcgcgc atcgtcgagg tgaacggcat gttgagattc tagctgcagc
     1201 ggagcggtta cgcgcgcaag ctcccgccgc gagattactt aatcagccag gctgcgccac
     1261 gctcaggcca atcgactcgc cagctcgtcc gcatgatgca tgagctgcgc gaagggggcc
     1321 gtgccggtga cctgccggct caatagcgcc tgcagatctt ccgcggtgca gggctcgcca
     1381 aacagtttgc cctgtgcgta gtggcaggcg ttacgacgca ggaaatccag gtgccgcagc
     1441 gactcgacac cttcggcgac cacttccatg ccgaggctgc gccccatctc gatgatcgtc
     1501 ttcacgatgg tcgcatcgcc ggcgtcctcg ctgacattgc gcacgaaaga ctgatcgatc
     1561 ttgagcgtac cgatggggaa ctgttgcagg gcggagagcg aggagtagcc cgtaccgaaa
     1621 tcgtcgatcg acaagtgcag tcccatggca tagagctcat cgagcagccg cacggtgcgc
     1681 ttggcgtcgg ccatcaaagt cgtttcggtg atctccagct cgaaagaggt cggcgagacc
     1741 gaatgccggc ggaacaccga gcggcagcgc aggatgaagc tcgcctggcg cagttgcttg
     1801 agcgacaggt tgagcgagat gcgccccggg ctggcccctt cgctctgcat gcggcggtaa
     1861 tcgagacaga cgcgattcag cacccactcg ccgatgtcca ggatcaggtt ggtttcctcc
     1921 gcaagcggaa tgaacatgcc cggcggaatg tcgccatggc cggggagccg ccagcgcagc
     1981 agcgcctccg caccgatgat tcgaccattg cgcagatcga ccttgggctg atagcggatc
     2041 accagctcgt cacgctcgag cgcgcgacgc agcttgctct tcaacatcag ccgctcgacc
     2101 gccgcagcat tcatctccgg cgaatagaac gcgaaggtgt tgccgccgtt ctgcttcgag
     2161 tagtacatcg ccgcgtcggc gttgcggatc agatcgatga cgttttcggc atcgcgggga
     2221 caaaatgcga tgccgatgct ggccgtcagg aagacctcgt gctggttgag ttggaaggcg
     2281 cgcgcgactt cggtgaggat cgagcgcgcc agatgggcga tcgggccgcg gttgtcggca
     2341 tcggccggaa ggccgtccac gaagagtgcg aattcgtcgc cagccagacg cccgaggatc
     2401 gtgtccttcg gcagaaggcg tgtcagtcgc tcggccagaa cctcgagcag ccggtccccg
     2461 gaggcgtgtc cgaacgtatc gttcacctcc ttgaagcgat ccatgtccag gtacagcagc
     2521 gccagcacct gtccgctgcg cagtccgcgt gcgatggtct gctgcagcag atgatggaac
     2581 tgcatccggt tggggatctt cgtgagcgca tcgtagcgcg cgagatagcg gatgcgccgt
     2641 tccgcacgct tgcgatcggt gatgttgcgg gcgacgaaga tatttccctg gaactgcgga
     2701 tcgtcggtct cgatgcgtga tccggtgaag gagaccggaa tggtctgccc gccgcgcgtg
     2761 cgcaccacgg tctcgcgcgt ctcctgggta gcctgcaaca gatcgaagct gtcccgctcg
     2821 cgctcatcga gcattgcaat gatgctgcgc ccgagaattt cctcctccgc atacccgagc
     2881 agcttgcagg ccgcggagtt cgcgaccttg accacgccat cgggcgaggt cacgaagacc
     2941 gcgtcggtca tgctgttgag cacgctgtgc agatagttct tgtggaccgt ggattgacgc
     3001 agccgaccgc gcatcctctc gagggccatc tgcagctcgc cgagctcatc ccggcggcgt
     3061 acgtcgacgg gccgcgtgta gtcgccctgt ccgatccgct cagcgccgcg gatcatggcc
     3121 acgatcggct gctcgagctt gcggcctgca cgccaggcca gggcggcgcc gagtagccca
     3181 ccaccgagtg ccaggccacc ggcaatcagc agcgcgagtt gcatctgctc gctgttggcc
     3241 gcggccaggt ggcggcccag actcatggcg ggactgggcg cgatctgatc cacgaggatg
     3301 cgcagctggc caaaggtctt cggcgtcgcc gcacccggaa tgctctcgac catctcccgc
     3361 acgggctgcg ccgcttcgaa ctgcagggtc gcgcgcgacg gagtcgggcg gcgccagcga
     3421 taaagcgttg ctccgtcgga gtcggtgacc gtcacctcgg ccacggtgcg gtcctcgagg
     3481 aaaggctgca gccggtgcgc gatcgattcg gtgtcgtggg cgcggacggc acccgccacc
     3541 gcttccgcgg catgcgcagc cgtggactcg gcacgggcgc gtagctccac cgcgacacga
     3601 tcgcgcgcgg aattctcagc gacaatgccg agtcgtcgcg tgcctacgtc atcctgccaa
     3661 gccagcaccg ccgtcgccag gatcgtgacg aggaggacga ccacgaaggc cgcgagggcg
     3721 tatttcaact tgagactggc ggcgctcaca atgaatat
//
LOCUS       DASRHN010000284         6982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1744805, whole genome shotgun sequence.
ACCESSION   DASRHN010000284 DASRHN010000000
VERSION     DASRHN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744805"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VFB37_11425"
     CDS             complement(<1..1266)
                     /locus_tag="VFB37_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZO23102.1"
                     /translation="MNANVVELPDSVTEVTARNENLPPPAVSTPVLTMPGLAAPSVSP
                     SLPTPCAEQLTQINESLARREALLAASAKASRLLLEAPDVRAVVPDVLRLIGEPAGVD
                     RVSLMLSQAGPGGEPLLVVASEWVAEGVAPYLEEPSRCSCDERDFSALCTELRAGRSV
                     CLSMDECPTNFSAISFEGRGTKTKAIVPVFVAGEFSGVVGFDNTRQRRAIDSAELSAL
                     ETAAGVIGAALHRERLVDDVRRTRERAAEEKVTELAKANAVIRGNLERLASEPDLHSF
                     MGHMLLETTRQFDAASGSVIVSKDSLQEWRILAHVRAGQLESPPFAASVPFAGSPFTE
                     VFGRSPKAAYMNVQQGNWEIWPGALDYHRREGFVSVLIYPLVFGARNVGFFVLSFRRE
                     APEVQHSELLVALAQQATLAVQLTRLAYSA"
     gene            complement(1375..2805)
                     /locus_tag="VFB37_11430"
     CDS             complement(1375..2805)
                     /locus_tag="VFB37_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO23103.1"
                     /translation="MNNLNIKGTVALLLTASLAACAVGPNYKQPQTSVAKSFAAAEPD
                     VYSSEQTQVQFWKQFNDPTLDKLVDDALLANHDLRIALGRLAEARAARHQSLYDLAPT
                     VTASASHQTQKLPAVEAGFPFTASYYDAGFDAFWELDLFGRIRRNTEAALADQQSAEA
                     NLRDVQVSVIAEVARTYFELRGEQNQLAVAQQNVQNQQETFHLTSARLDAGRGTELDT
                     SRAQAQLSTTLSTVGPLQAAVSRSTHRLSVLTGREPDALQDVLSAPRDLPPLPEVAAV
                     GNPADLLRRRPDIRVAERQLAASTARVGVAVGDYFPKVTFTGSFGYDAASPNGLGTSA
                     SRAYQIGPGISWAAFDLGRVHAEVAGARARNDTALAEYEQTVLRALEETENALVTHAR
                     TRDELAHAVDAARASGAAAKLARTRYEGGVIDFLEVLDAERTQLEAEDRLAQSRTDAA
                     TSLVAVYKALGGGWEGAPLPRYTRAN"
     gene            complement(2814..6026)
                     /locus_tag="VFB37_11435"
     CDS             complement(2814..6026)
                     /locus_tag="VFB37_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZO23104.1"
                     /translation="MKISNFFIERPIFAGVISAFITIAGLIALFKLPISEYPEVVPPQ
                     VVVHASYPGANPKVIASTVAAPLEQAIVGVEDMLYMSSTSTMDGALTLTITFRNGADI
                     DRAQVQVQNRVSQALPRLPEEVRDLGVTTVKASSNLTLVVNLTSPNGRYDSLYLRNYA
                     VLNIKDVLARLPGMGDVQVFGGGDYSMRIWLDPQKLASRNLTASDVVAAIREQNVQVA
                     AGQLGAPPSNSTEFQVALNAAGRLTDEQQFRDIVVKTGSDGEVVKLGDVARVELGAQA
                     YGVRSFLDNKPSIAIPVFQSPGANALELSTNVRKTMEELKKNFPEGMDYGILYDPTQF
                     VRQSIDAVLHTLLEAVALVVLVVIVFLQTWRASLIPLIAVPVSVIGTFAVLLGFGFSI
                     NTLSLFGLVLAIGIVVDDAIVVVENVERHIAEGLTPREATKKAMSEVSRPIIAITLVL
                     CAVFGPVAFVPGLTGEFYRQFALTIAISTVISAFNSLTLSPALAAVLLRPHGAKQDFL
                     SRLMNTVLGRFFKRFNRGFTNAGNSYSRTLTITMRHGGIALVIYAGLIVLTYFGFNAV
                     PGGFIPEQDKQYLVGVAQLPPAASLERTDAVTRRISEIGLKQPGVAHAVAFAGMSVNG
                     FTQSSSAALIFFPLDDFHERTSRNLSGHAIAAALNQKMASIQDAFVMVVTPPPVIGLG
                     TLGGFKLEVEDRGDAGDQALFAALSEALGKANKNPALGGAFSTYQINVPQLNVDVDRV
                     KVKRQNLKLSDVFETLQVYLGSLYVNDFNRFGRTYQVVAQADAPFRAHLDDVLPLKTR
                     NANGEMVPLGSVLSVSQSFGPDVVQRYNAYRAADVNGGPAPGYSSGQAQAAIAKILDE
                     TLPRGMSYEWTDLAYQQLLSGGSAYLVFPLCVLFVFLVLAAQYESLSLPLAIILIVPM
                     CLLPAITGVWLDHGDNNIFMQIGLLVLVGLACKNAILIVEFAKHLQEEQGRDARTAVL
                     EAAHLRLRPILMTSIAFIMGVVPLVLAQGAGAEVRHSMGVTVFAGMIGVTLFGLLLTP
                     VFYVLVRRLTSKSGAYAVAETDSTDAGTRGGSIGHEAHQV"
     gene            complement(6110..>6982)
                     /locus_tag="VFB37_11440"
     CDS             complement(6110..>6982)
                     /locus_tag="VFB37_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO23105.1"
                     /translation="AKAQLAQARTQRELAKSEKDRAVKLLAQHAISQEEYDTRTAGSE
                     QADANVEAAQAALDSSALNLEFTRVTAPITGVVGRAEVTEGNFVSSGQTRLTTLVSTD
                     PIYVSFDGDEQAYLKYMEFARNPHTDNKQPVWVGLADENGYPHKGVMVFVNNEIDAQT
                     GTVRARGSLENHDHQFTPGMFARVKLPGGPAHDALLINDSAVGTDQSAKYVLVVGKDN
                     KVEYRPVKLGPIVDGLRVVREGLSPGDTIVVNGLQRVHPGTPVTPQRVAMGEQHPANG
                     TDPGSMVAQNSKVE"
BASE COUNT         1251 a         2171 c         2381 g         1179 t
ORIGIN      
        1 cgccgagtag gcgagtcgcg tgagctgcac cgcaagggtg gcctgctggg ccagggcgac
       61 gagcagctcg ctgtgctgga cctcgggcgc ctcgcgccgg aaggacagaa cgaagaagcc
      121 gacgttgcgg gcgccaaaga cgagcgggta gatcaggacg ctgacgaaac cctcacggcg
      181 gtgatagtcg agcgcaccgg gccagatctc ccagttgccc tgctggacgt tcatgtaagc
      241 agccttcgga ctgcggccaa aaacttccgt gaacggggac ccggcgaacg gcacgctcgc
      301 ggcgaagggt ggggactcca attggccggc tcggacatgc gccaggatgc gccactcctg
      361 cagggagtcc ttggagacga tgaccgagcc ggacgccgcg tcgaactgcc gcgtggtctc
      421 gagcagcatg tgccccatga agctgtgcag atccggctcg ctggccaaac gctccaggtt
      481 gccgcggatc acggcgttgg ccttggcgag ctccgtcact ttctcttcgg ccgcccgctc
      541 gcgcgtgcgg cggacgtcat ccaccaggcg ctcgcggtgc agggcggccc cgatcacgcc
      601 tgcggcggtc tcgagcgccg acagctcggc cgaatcaatc gcgcggcgct gacgcgtgtt
      661 gtcgaacccg accacgccgg agaactcgcc ggcgacgaag accggcacga tcgccttggt
      721 cttggtgccg cggccctcga agctgatcgc ggagaaattc gtgggacact cgtccatgct
      781 caggcacacg ctgcggccgg cgcgcagctc ggtgcagagc gcggagaaat cgcgctcatc
      841 acagctgcac cgggaaggct cttcgaggta gggcgcgaca ccctctgcga cccactcact
      901 ggccacgacc agcagcggct cgccgcccgg gcctgcctgc gagagcatga ggctcacgcg
      961 atcaacgccc gccggctcgc cgatgagacg taatacatcg ggcaccaccg cgcgcacatc
     1021 cggcgcttcc agcagcaggc ggctcgcctt ggctgaggcg gccagcaggg cttcccgtcg
     1081 tgcgagtgac tcgttgatct gagtgagctg ctcggcgcac ggtgttggca agctcggact
     1141 gacgctcggt gccgctaaac ccggcatcgt gagcacgggc gtggacactg ccggaggggg
     1201 gagattctcg ttgcgggcag tcacttccgt aacgctgtcc gggagctcga cgacgttggc
     1261 gttcatggca ggtgctccag atggatgccg gcagaaggac cgatctgggg gctggagaga
     1321 gtcagggcaa gctcatgcat ggcgggggtg gggacgcgat ttcccgaggg ggagtcaatt
     1381 tgcccgagtg taccgcggga ggggcgctcc ctcccaaccg ccacccagcg ccttgtacac
     1441 ggccaccagg ctggtggccg catccgtgcg gctttgggcc aggcgatcct cggcctcgag
     1501 ctgggtgcgc tcggcatcga gcacctccag gaagtcgatg acgcctccct catagcgggt
     1561 gcgggccagc ttggccgcgg caccactggc tcgagccgca tcgacggcgt gcgccagctc
     1621 atcacgagtg cgggcgtggg tgaccagggc gttctcggtt tcttccagcg cgcggagcac
     1681 cgtctgctcg tactcagcca gagccgtgtc gttgcgggca cgggcgccag cgacttcagc
     1741 atgcactcgg ccgagatcga aagcggccca ggagatgccc gggccgatct ggtaggcgcg
     1801 gctggcggag gtaccgagac cattcgggct cgcagcgtcg tagccgaagc tgcccgtaaa
     1861 cgtcactttg gggaagtagt ctccaactgc gacgcccacg cgtgcggtgg aggctgcgag
     1921 ctgccgctcc gcgacgcgga tgtccggccg gcgtcgcaac agatcagcgg ggttgccaac
     1981 cgcggccact tcgggcaggg gcggcagatc ccgtggtgcg ctcagcacat cctgcagggc
     2041 atccggctcc cggcccgtca acacgctgag ccgatgggtc gagcgcgaga ccgcggcttg
     2101 cagcggcccg acggtcgaga gcgtcgtgct cagctgggcc tgggcgcgcg aggtgtccag
     2161 ctccgtgcct cttccggcat ccaggcgcgc cgaggtgagg tggaaagtct cctgctggtt
     2221 ctgcacgttc tgctgcgcaa ccgcgagctg gttctgctcg ccccgcagct cgaaataggt
     2281 gcgcgcgacc tcggcgatga ccgagacctg gacgtcccgc aaattcgcct ccgcgctttg
     2341 ttggtccgcg agcgccgcct cggtgttgcg gcgaatgcgc ccgaagaggt ccaactccca
     2401 gaatgcatcg aagccggcgt catagtagga ggcggtgaag gggaagcccg cttccaccgc
     2461 cggtagcttc tgggtctggt gactggcgga ggccgtcacg gtcggtgcca ggtcatagag
     2521 agactgatgc cgagccgcgc gcgcctcggc aaggcgtccc agggcgatac gcaggtcgtg
     2581 attagcgagc agcgcatcgt ccacgagctt gtcgagtgtc ggatcgttga actgcttcca
     2641 gaactgcacc tgggtctgtt cgctggagta gacgtccggc tccgcggccg cgaagctctt
     2701 cgcaacggaa gtctgcggct gcttgtagtt ggggccgacg gcgcaggcag ccagcgaagc
     2761 ggtcagcagc agtgccacgg tgcccttgat gttgagattg ttcatgatct gcctcaaacc
     2821 tgatgggcct cgtggccgat ggagccgcca cgcgtgcccg catcggtgct gtcggtttct
     2881 gcaacggcat acgctccgga cttggacgtg aggcgccgga cgaggacgta gaacaccggg
     2941 gtgagcagca gcccgaagag ggtcacgccg atcattccgg cgaaaacggt gacacccatc
     3001 gagtggcgga cttcggcacc tgcgccctga gccagcacca agggcacgac acccatgatg
     3061 aaggcgatcg atgtcatgag gatggggcgc aatcgcaggt gagcggcctc gagtaccgcg
     3121 gtccgggcgt cccggccctg ctcctcctgc agatgcttgg cgaactccac gatcaggatg
     3181 gcgttcttgc aggcaagtcc caccaacacg agcaggccaa tctgcatgaa gatgttgtta
     3241 tcgccatggt cgagccagac gcccgtgatc gctggcagca gacacatcgg cacgatgaga
     3301 atgatggcca gcggcaggct gaggctctcg tactgggccg ccagcaccag gaacacaaac
     3361 agcacgcaca acgggaacac gaggtacgcg ctgccgcccg agaggagctg ttggtaggcg
     3421 agatcggtcc actcgtacga catgccgcga ggcaaggtct catccaggat cttggcgatc
     3481 gccgcctgcg cctgacccga gctgtacccg ggagcagggc ccccgttcac gtccgcggca
     3541 cggtacgcgt tgtagcgctg cacgacgtcc gggccaaagg actgcgacac gctgaggacg
     3601 gagccgagcg gcaccatctc gccgttggcg ttgcgagtct tgagcggcag gacatcatcg
     3661 agatgagccc ggaacggcgc atccgcctgc gccaccacct ggtaggtgcg gccaaaacgg
     3721 ttgaagtcgt tcacgtagag tgagccgaga tagacctgca gcgtctcgaa cacgtccgag
     3781 agcttcaggt tctgccgttt caccttgacg cgatccacat ccacgttcag ctgcggaaca
     3841 ttgatctggt aggtgctgaa ggcgcctccg agggcggggt ttttgttggc cttgcccagg
     3901 gcttcggaga gcgctgcgaa cagggcttga tcgccggcat cgccacgatc ttcgacctcg
     3961 agcttgaatc cgccaagggt tccgagtccg atcaccggag gtggggtgac gaccatgacg
     4021 aaggcatcct ggatggaggc catcttctgg ttgagcgccg ctgcgatggc atggccggac
     4081 agattgcgcg acgtgcgctc atggaagtca tcgagcggga agaagatcag ggcggcgctc
     4141 gagctctgtg tgaagccgtt gacggacatc cccgcgaagg ccacggcgtg cgccacaccc
     4201 ggctgcttca agccgatctc ggagatgcgg cgggtcactg catccgtccg ttcgagtgag
     4261 gctgcagggg gcaactgcgc gacaccgacg aggtactgct tgtcctgctc cgggatgaag
     4321 cctcccggga cggcgttgaa gccgaagtac gtgaggacga tcagtccggc atagatgacg
     4381 agcgcgatgc cgccatggcg catcgtgatg gtcagcgtgc gggagtagct gttgccggca
     4441 ttcgtgaagc cccggttgaa ccgcttgaag aaacggccca gaaccgtgtt catcaggcgc
     4501 gacaggaaat cctgcttcgc accgtgcggg cggagcaaca cggctgccag tgcgggggac
     4561 aaggtgagcg agttgaaggc cgagatgacc gtcgagatcg caatcgtgag cgcgaactgg
     4621 cggtagaact cgcccgtcag gccgggcacg aaggccacag ggccaaagac ggcgcacagg
     4681 accagcgtga tcgcgatgat cggccggctc acctcgctca tggccttctt cgtggcctct
     4741 cgcggtgtga gtccttcagc gatgtggcgt tcgacgttct cgaccacgac aatcgcatcg
     4801 tcgaccacga taccgatcgc gaggaccaag ccaaacagcg acagcgtatt gatggagaag
     4861 ccgaagccca ggagcacggc gaatgtgccg atgaccgaca ccgggacggc aatgagcgga
     4921 atgagagagg cgcgccaggt ctgcaggaag acaatgacga ccaggacgac caacgcgacg
     4981 gcctcgagca gcgtgtgcag cacggcatcg atcgactgcc gcacgaactg cgtcgggtca
     5041 tagaggatcc cgtagtccat accctcgggg aagttcttct tcagctcctc catcgtcttg
     5101 cgaacgttgg tggacagctc gagcgcattc gcacccgggg actgaaagac cgggatggca
     5161 atcgaaggct tattgtcgag aaaactgcgc acgccgtagg cctgcgcgcc gagctcgacg
     5221 cgagcgacgt cgcccagttt gacgacctcg ccgtcggaac cggtcttgac cacgatgtcg
     5281 cggaactgct gctcgtccgt aaggcggccg gccgcattca gcgcgacctg gaactcggtg
     5341 ctgttcgaag gcggcgcgcc gagctgaccg gccgcgacct ggacgttctg ctcgcgaatg
     5401 gccgcgacaa catcactggc cgtcagattg cgggaggcga gcttttgcgg gtcgagccag
     5461 atccgcatcg aatagtcgcc accgccgaat acctgcacat cgcccatgcc gggcagccgc
     5521 gccagcacgt ccttgatgtt gaggacggcg tagttgcgca gatagagcga gtcgtagcgg
     5581 ccattcggtg aggtcaggtt gacgacgagc gtcagattgg aggaggcctt gacggtcgtg
     5641 acgcccagat cgcgcacttc ctcaggcaga cggggtagag cctgggaaac gcggttctgc
     5701 acctgcacct gggcgcgatc gatgtcggcg ccgttgcgga acgtgatggt cagggtcagc
     5761 gcgccatcca tcgtggacgt cgaagacata tagagcatgt cttcgacgcc tacgatcgcc
     5821 tgttcgagcg gcgcagcgac ggtcgaggcg atgactttcg gattggcgcc agggtaggaa
     5881 gcgtgcacga cgacctgcgg cggcacgact tcggggtact cactgatcgg cagcttgaag
     5941 agcgcgatca ggcccgcgat ggtgatgaac gccgagatga cgccggcgaa gatcgggcgc
     6001 tcgatgaaga agttggaaat tttcaccgtc gttcctcggt tgattcaggt gtcaggcaca
     6061 aagaagggaa gagcgagacg gccgggccag gcccggccgt gccgcgccgt cattccacct
     6121 tggagttctg cgcaaccatg ctgcccgggt cggtgccgtt ggccggatgc tgctcgccca
     6181 tcgccacgcg ctgcggtgtc accggcgttc ccgggtgaac acgctgcagg ccgttcacca
     6241 cgatcgtgtc gccgggggac aagccttcac gcaccacgcg caggccatcc acgatcgggc
     6301 cgagcttcac cgggcggtac tcgaccttgt tgtccttgcc tacgacgagc acgtatttcg
     6361 cgctctgatc ggtgcccacc gcgctgtcgt tgatcagcag cgcgtcgtgc gctggcccgc
     6421 cgggcagctt cacccgagcg aacatgcccg gggtgaactg gtgatcgtga ttctccagcg
     6481 agcctcgggc gcggaccgtg ccggtctgtg cgtcgatctc attgttgacg aagaccatca
     6541 cgcccttgtg cggatagccg ttttcgtccg cgaggcctac ccagaccggt tgcttgttgt
     6601 cggtgtgggg attgcgcgcg aactccatgt atttgaggta ggcctgctcg tcgccatcga
     6661 aggacacata gatgggatcc gtcgaaacca gcgtggtgag ccgggtctga ccgctggaga
     6721 cgaagttgcc ttcagtgacc tcagcgcggc ccacgacgcc tgtgatcggc gcagtcaccc
     6781 gagtgaactc gaggttcagg gccgaggaat ccagggcagc ctgcgcggct tcgacgttgg
     6841 catccgcctg ctcgctacct gctgtgcgcg tgtcgtactc ctcctgcgag attgcgtgct
     6901 gggccagcag cttcacggcg cgatcctttt cggatttcgc cagctcacgc tgggtgcggg
     6961 cttgcgccag ttgcgccttg gc
//
LOCUS       DASRHN010000285         7091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2077452, whole genome shotgun sequence.
ACCESSION   DASRHN010000285 DASRHN010000000
VERSION     DASRHN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077452"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFB37_11445"
     CDS             <1..878
                     /locus_tag="VFB37_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO23106.1"
                     /translation="LELSSQLSARLSLEHRLRGALENQQYTLYYQPKFDIATGRIEGV
                     EALLRWNDPERGVISPAEFLPILESTGMIVAVGDWAVQQAAADCQRWLAAGLHPVHIA
                     VNCSPLQLRKPGFAEHVLAALDGWGHDGWGIDLEITESLLIDPDSSEVHKLRALRAAG
                     VRVAIDDFGTGYSSLSRLSDLPVDTLKIDRSFIKGLPRDRASARLVPTIIGLARAFDL
                     VTVAEGVESRAQLRFLLGAGCQQSQGYLHARPMRATDLEALLAAQARHPASATQHTQQ
                     QDSEKLDALLSRQQK"
     gene            complement(953..1741)
                     /locus_tag="VFB37_11450"
     CDS             complement(953..1741)
                     /locus_tag="VFB37_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZO23107.1"
                     /translation="MQIVAITNIKGGVGKTTTAVNLAYLCSASGRATLLWDLDPQGAA
                     SYTLRCEPNGQASAKKLLAGKRELPELVVPSGYPELDVLPADFSYRKFDVHLSMRKHP
                     TERLLRMSRAVRDEYDVLFLDCPPGISLLSENVLRAADVVIVPLIPTPLSIRMLIQLQ
                     EFIAAEGWTDVALVPFFSMVDRRKSLHHEVIATAREQFPALLASEVPYSSEIERMSLR
                     RAPLPAFSPRSAVGLIYASLWQELVRFLGEARPRSMRELDATAS"
     gene            1887..2411
                     /locus_tag="VFB37_11455"
     CDS             1887..2411
                     /locus_tag="VFB37_11455"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZO23108.1"
                     /translation="MRVIPAVLAGALVLSATAAWAQGPTDPQIAAIVVTANQVDIDAG
                     KLAKSKASSKEVKSFAQTMISDHSGVNKSATELVTRLHVTPEPNATSENLKQGGEQNL
                     ANLRKLSGSAFDKAYIDHEVAYHQAVLDAMDKTLIPSAHNEDLKALLVKVRPAFVAHL
                     EHAKQLQASLGKSG"
     gene            2414..2809
                     /locus_tag="VFB37_11460"
     CDS             2414..2809
                     /locus_tag="VFB37_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HZO23109.1"
                     /translation="MPLRAGRDPRRQAPLAPASGLLRAGALLLGLSVLDICPGQSDSP
                     TPRGTESRTVTIENLQFTPQTLTVRRGDHVVWVNKDLFPHTVSSTEKKFDSRSIAPNS
                     SWTFVAKASGTFPYACTFHPTMTGTLVVQ"
     gene            2864..3511
                     /locus_tag="VFB37_11465"
                     /pseudo
     CDS             2864..3511
                     /locus_tag="VFB37_11465"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
     gene            complement(3580..3891)
                     /locus_tag="VFB37_11470"
     CDS             complement(3580..3891)
                     /locus_tag="VFB37_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HZO23110.1"
                     /translation="MIKVTVLYPNKAGATFDIGYYVSKHMPMVQRKCGSACRSIGAEH
                     GVGGGEPGAAPPYLALGYLTFDSVEAFQKAFNPHAGEILADIPNYTNTQPVIQISEIK
                     L"
     gene            3978..5084
                     /locus_tag="VFB37_11475"
     CDS             3978..5084
                     /locus_tag="VFB37_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23111.1"
                     /translation="MRKTPSLLLLLLACALTTPALALKTRNVVLIVSDGLRWQEVFTG
                     ADPLLLDAKHGGNWLDDTEMKRRYWRENPQERRSLLLPFIWGTVARQGQIFGNRSEGS
                     DAHVTNGRWFSYPGYNEMSVGYPNKAIDRNEFGLNPGVTVFEWLNGSPELHGKVAIYG
                     TWQVFADVFNEPRSHLPMQVGWNAPTRSSGSADKDALLARLYNTTTRFDADDSQDSLL
                     QVTLLDYVRSAHPRVLFVGYGETDNWAHQGRYDLVLESAQRMDGFVRELWETMQSMPE
                     YRDSTTFIITTDHGRGSGPVEWKDHGVEQKGSGNIWIAALGPDTPPLGERKHVGAVTQ
                     SQIAATVAAFAGKDYRREVPQAAPPIADLLGSHH"
     gene            5480..>7091
                     /locus_tag="VFB37_11480"
     CDS             5480..>7091
                     /locus_tag="VFB37_11480"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZO23112.1"
                     /translation="MAAVVFISVLTYSSLEARTTAAQKVTRTVTVLEELQAILSLTKD
                     AESGQRGFLITGEEAYLEPYTNAKSALTAQLESARRALATDPAQQRNMATLQQLLNDK
                     LTELAHTIALRRAGDTTAALNLVRSDRGRLLMESIRDVIGEMETTEHAALTRREEAWQ
                     RSETLSSAVIFGGSALLLVLIGAGAYMTSRDYRHRQREVWMRAGQMGMSAVIQGEQRL
                     ETLGERALAFLAEYLGVRVGALYIAEGAGTFRRVAGYALPSGSAPDVVRSGEGLLGQA
                     AKENRPLELADVPEGYLSVASTLGRGEPEAVLVAPASIDGSIQGVLELGFFRGIRTED
                     QELLGRLSESLGVAVRASKDRTRLEELLKETQRQGEELQSQQEELRVSNEELEVQARS
                     LKESQAQLEAQQTELEQTNAQLEEQAHVLARQRDELATAQSVLAQKAAALERANQYKS
                     EFLANMSHELRTPLNSSLILAKILADNRGGNLTADQVKYAQTISSAGHDLLAIINDIL
                     DLSKIEAGKVELTPQTIAVARVTEPLIKT"
BASE COUNT         1313 a         2335 c         2254 g         1189 t
ORIGIN      
        1 agctcgagct gagctcccag ctctccgcgc ggctgagtct cgagcatcgc ttgcgcgggg
       61 cgctcgagaa tcaacagtac acgctgtatt accagcccaa gttcgacatt gccaccggcc
      121 gcatcgaggg cgtcgaggcc ttgctgcgct ggaacgatcc cgagcggggc gtgatctcgc
      181 ccgctgagtt tctgcccatt ctcgagtcca ccggcatgat cgtcgcggtc ggtgactggg
      241 ccgtgcagca ggccgcggcg gattgccaac gctggctcgc cgcaggcctg catccggtgc
      301 acatcgccgt gaactgctcg ccgctgcagc tgcgcaagcc cggcttcgcc gagcatgtgc
      361 tcgcggcgct cgatggctgg gggcacgatg gctgggggat cgatctggag atcaccgaga
      421 gtctgctgat cgatccggac tcttcggagg tgcacaagct gcgtgcgttg cgtgccgccg
      481 gcgtacgcgt cgccatcgat gacttcggca ccggctattc ctccctcagc cgcctgtccg
      541 atctgccggt ggacacactc aagatcgatc ggtccttcat caaaggtctg ccgcgggacc
      601 gggcctccgc gcgcctcgtt cccacgatca tcggactggc gcgcgccttc gatctggtaa
      661 cggtcgccga aggcgtcgaa agccgggcac agctgcggtt tctgctcggg gcgggttgcc
      721 agcagtccca gggttacctg catgcgcgac ccatgcgtgc gacggatctg gaagcgcttc
      781 tcgccgcgca ggcacggcat ccggccagcg ccacacaaca cacacaacaa caggactccg
      841 agaagctcga tgcgctattg tcgcggcagc agaaatagcc gtcggcacgg tgaaacttcc
      901 cgccgcatcc ctcgagccct agaggccgcc ggacctgtcc gcgcggcttt cctcaactcg
      961 ccgtagcgtc gagctcacgc atcgatcgag gtcgcgcctc gccgagaaag cgcaccagct
     1021 cctgccagag cgaggcgtag atcagtccca cggcgctgcg aggcgagaag gcgggcaacg
     1081 gcgcgcggcg caggctcatg cgctcgatct cgctggaata cggcacctcg ctagccagca
     1141 gcgcggggaa ctgctcgcgc gcggtcgcga tgacttcgtg atgcagtgac ttgcgacgat
     1201 cgaccatgga gaagaatggc acgagcgcca cgtcggtcca gccctccgcg gcaatgaact
     1261 cctgcagctg gatgagcatg cggatcgaca acggtgtcgg aatcagaggc acgatgacca
     1321 cgtcagccgc gcgcagcacg ttttccgaca gcagcgagat gccaggtgga caatcaagaa
     1381 acagcacatc gtactcatca cgtacggcgc gactcatcct gagcagccgc tcggtcggat
     1441 gcttgcgcat gctcagatgc acgtcgaact tacggtacga gaagtctgcc ggcagcacat
     1501 ccagttccgg gtatccgctc ggcaccacga gctcgggcaa ctcgcgcttg ccggcgagca
     1561 gcttcttcgc ggaagcctgc ccgttcggct cgcaacgcaa cgtgtaactc gctgcgccct
     1621 gagggtccag atcccacagc agtgtcgccc ggccgctggc actgcacaga taagccaggt
     1681 tgacggcggt cgtcgtctta ccgactccgc ccttgatgtt cgtgatggca acgatctgca
     1741 tcgtcttggt cggtcaatgt cgcactgagc tatattagcg cgctcgattg gctgcacgaa
     1801 gctctcatcc gcttcgggac ttcggaacgg gacgctctca cgtgacatcc aatgtccgtg
     1861 cgaacccctt ttccaggaga acaatcatga gagtgatccc cgcagtgctg gcaggcgcgc
     1921 tggtcctgtc ggccaccgcc gcctgggcgc agggcccgac cgacccgcaa atcgccgcca
     1981 tcgtggtcac ggcaaaccag gtggacatcg atgccggcaa gctcgcgaaa tccaaggcga
     2041 gctcgaagga agtcaaaagc tttgcccaga cgatgatctc cgaccacagc ggtgtcaaca
     2101 agtctgcgac cgagctggtg acccgactgc acgtcacgcc ggagccgaac gcgaccagcg
     2161 aaaatctcaa gcagggcggc gagcagaacc tggccaatct gcgcaagctc agtggcagtg
     2221 ccttcgacaa ggcgtatatc gaccatgagg tcgcctatca ccaggccgtg ctcgatgcca
     2281 tggacaagac gctgattccg agcgctcaca acgaggatct caaggctctg ctcgtcaagg
     2341 tccgtcccgc gttcgtcgct catctggagc atgccaagca gctgcaggcc tccctcggca
     2401 agagcggttg aagatgccgc ttcgcgcagg ccgcgatccc aggcgccagg cacccctggc
     2461 gcctgcttcc ggtctgctgc gggctggcgc tctcctcctc ggtctgtctg tcctggacat
     2521 ctgcccgggg cagtccgact cgccaacacc gcgcgggacc gagagccgca cggtgacgat
     2581 cgaaaatctg caattcacgc cgcagaccct cacggttcgc cgcggagatc acgtcgtctg
     2641 ggtgaacaag gatctgtttc cccacaccgt cagctcgacg gagaagaaat tcgactcgcg
     2701 cagcattgca cccaacagct catggacgtt cgttgcgaaa gcgagcggca catttcccta
     2761 cgcctgtacg tttcacccta ccatgacggg aacgctcgtc gtgcaatgac ggcgcggaga
     2821 taaccatgac caccgacagc tcgggccagc actcgaaact agcagcgatg atgcctcgct
     2881 ggcgcggcgc atcgcccagc gcgaccgggg cgctttcgca actctcatgc gccgctacaa
     2941 ccggcgcctg tacagagtcg cacgcgccac gctgcacgat gaggccgagg cccaggatgc
     3001 cttgcaggaa gcgtatctgc gcgcctaccg ttccatcgaa cagttccggg gcgaggcggc
     3061 gctgtccacc tggctcaccc gtctggtgct caacgagtgc ctggctcgtc tgcgacgcga
     3121 tgcccgtcgg cagaacgtcg tacccctagt gagatccaac acggagaccg ccatggccag
     3181 cacgcccgca ccggagtgcg agcagcccga gaaatcggcg ggggtcgcgc agatgcgggc
     3241 cctgctggag cgcaagctgg atgaactgcc ggagcacttc cgcacggtat tcgtgctgcg
     3301 ctcggtggag gagctgagcg tcgaggagac cgcacaatgt ctcgacattc cccccgagac
     3361 ggtccgcacg cgacactttc gggccaagag cctgctgcgc gaagccctgg cccaggagat
     3421 cgatctcgcc gagcgcgatg tgttcgagtt cggtggcgag cactgcgacc gtacggtcgc
     3481 gaacgtactg cagctcctcg ggcccgcctg agccgccggc gccgggtcag cctcactccg
     3541 cgtgcgcccc actcgcgggg cacgcaatcc gacactctgc tagagcttga tctcgctgat
     3601 ctggatcacc ggctgcgtgt tggtgtagtt cgggatgtcg gccagaatct cgccggcatg
     3661 gggattgaag gctttctgaa acgcctcaac tgagtcgaac gtcagatatc cgagcgccag
     3721 gtatggtggt gcggcccccg gctccccgcc tccgactcca tgctcggctc ctatgctgcg
     3781 gcacgcggag ccacacttac gttgcaccat cggcatgtgt ttgctgacgt aatagccgat
     3841 gtcgaacgtt gcgccggctt tgttcgggta tagtacggtg accttgatca ttggacggct
     3901 cctgcgcggt ttgcacgcgc cagtttgccc ccccgtgagc gacaatgcca ccccgagacc
     3961 gcaatatccc gagcgccatg cgtaaaacac cctccctgct gctcctgctg ctggcctgtg
     4021 ctctgaccac ccccgcgctg gcactgaaaa cccgcaacgt ggtgttgatc gtgagcgacg
     4081 gcctgcggtg gcaggaggtc ttcacgggtg ctgatccgct gctgctcgat gccaagcacg
     4141 gcggcaactg gctggatgat acggaaatga agcgccgcta ctggcgtgag aatccccagg
     4201 agcgccgcag cctgctgctg cctttcatct ggggaacggt ggcccggcag gggcagatct
     4261 tcggcaatcg cagcgagggc agcgatgcgc atgtgaccaa cggccgctgg ttctcgtatc
     4321 cgggttacaa cgagatgtcg gtcggctatc ccaacaaagc gatcgaccgg aatgagttcg
     4381 gcctcaatcc aggtgtcacg gtgttcgaat ggctgaacgg ctctcccgag ctgcacggca
     4441 aggtggcgat ctatggcacc tggcaggtgt ttgccgatgt cttcaatgag ccgcgcagtc
     4501 atctaccgat gcaggtgggc tggaatgcac cgacgcgcag cagcggcagc gccgacaagg
     4561 atgcgctgct cgcccggctg tacaacacga ctacccgctt cgacgcggat gattcgcagg
     4621 actccctcct gcaggtgacg ctacttgact acgtccgctc tgcccacccg cgggtgctgt
     4681 tcgtcggcta tggcgagacc gacaactggg cgcatcaggg acgctacgat ctggtgctcg
     4741 agtccgcaca gcgcatggat ggattcgtgc gcgagctgtg ggagaccatg cagtcgatgc
     4801 ccgagtaccg cgacagcacg acattcatca tcaccaccga tcacggtcgc ggcagcgggc
     4861 cggtcgaatg gaaagatcac ggggtcgagc aaaagggctc cggcaacatc tggatcgcgg
     4921 cactgggtcc ggatacaccg ccgctggggg aacgcaaaca cgtcggcgcc gtcacgcagt
     4981 cgcagatcgc cgcgacagtc gcggcgttcg ctggcaagga ctaccgccgc gaagtcccgc
     5041 aagccgcccc gccgatcgcc gaccttctgg gttcacatca ctgaccgggg ccgggggcag
     5101 tgggattgcg ccgcgccggt cggggcggtg cgagctcagt tcgcgccaac ctgccgcaag
     5161 cgctgaggag gctgggcatc ccgccgctag agccgcccgc atcccctcgt accaggtgtg
     5221 gggacagcgc tcccgggtcc ctgccccgtc caagcttaaa agctgtttgc ggtcatcgaa
     5281 aagcgtgatt cgcccctgag gcgatgggtc tgccccccgg aagccgcctt cgatgaatgc
     5341 ccgggtggac gataaactgc aggctcgcgt gcctgggatg acttgaatga agccaatgtc
     5401 gggtcctgct catcattccg agggttctcg cgccggagcg ccgctgccgc cggggcctct
     5461 gctcggattt gtgattgccg tggccgccgt cgtgttcatc tcggtgctga cctacagctc
     5521 actggaagca agaacgaccg cagcgcagaa ggtgacgcgc accgtgaccg tcctggagga
     5581 gctgcaggcg attctctcgc tcaccaagga tgccgagtcg ggccagcgcg gcttcctgat
     5641 cacgggagag gaggcctacc tcgagcctta caccaatgcc aagtcggccc tgacagcgca
     5701 gctcgaaagc gcccggcggg cgctggcaac cgatccggcg cagcagcgca acatggcgac
     5761 tctccagcag ctcctgaacg acaagctgac cgagctggcc cacacgatcg cactgcgccg
     5821 tgccggcgac acgaccgccg cactgaacct ggtgcgcagc gatcgaggca gattgctcat
     5881 ggagagtatc cgcgatgtca tcggcgagat ggagacgacc gagcacgccg cgctcactcg
     5941 gcgcgaagag gcctggcagc gcagcgagac actctcctcg gcggtcattt ttggcggctc
     6001 agcgctgctg ctggtgctca tcggcgccgg cgcgtacatg acctcgcgcg actatcgcca
     6061 ccgccagcgt gaggtttgga tgcgagcggg ccaaatggga atgagcgcgg tcatccaggg
     6121 cgagcagcga ctcgagaccc tgggcgagcg agctctggcg tttctcgccg aatatctcgg
     6181 cgtacgggtg ggcgcactgt atatcgccga gggagcgggc acgttccggc gcgtcgcggg
     6241 gtacgcgctc ccctccggat ccgctcccga tgtggtgcgt tccggggagg gtctgctggg
     6301 gcaggccgcg aaggagaatc gaccgctgga gctcgccgac gtgccggagg gctatctgag
     6361 cgtggcctcg actcttggca gaggcgagcc cgaggccgtg ctcgtcgcgc cggcgagcat
     6421 cgacgggagc atacagggcg ttctcgagct gggattcttt cgcggcatcc gcaccgaaga
     6481 ccaggagctg ctcgggcgtc tctccgagtc cctcggcgtg gcggtgcgcg cctcgaagga
     6541 ccggacccga ctcgaagagc tgctgaagga gacacagcgt cagggcgagg agttgcagtc
     6601 gcagcaggaa gagctgcgtg tcagcaacga ggagctggag gtccaggcgc gctcgctcaa
     6661 ggaatcacag gcacagctcg aagcgcagca gaccgagctc gaacagacca acgcacagct
     6721 cgaggagcag gcgcatgtgc tggcccgcca acgtgacgag ctggccacgg cgcaatccgt
     6781 gctggcccag aaggcggccg ccctcgagcg agccaaccag tacaagagcg agttcctcgc
     6841 caacatgagc catgagctgc gcaccccgct caactcctcg ctcatcctcg cgaagatcct
     6901 ggccgacaac cgcggcggca atctgacggc ggaccaggtg aagtatgccc agacaatttc
     6961 ctcggcgggt cacgacctgc tggcgatcat caatgacatc ctggacctgt cgaagatcga
     7021 ggccggcaag gtcgagctga caccccagac catcgccgtc gcccgggtga ccgaaccgct
     7081 gatcaagacc t
//
LOCUS       DASRHN010000286        23034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_591425, whole genome shotgun sequence.
ACCESSION   DASRHN010000286 DASRHN010000000
VERSION     DASRHN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23034
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591425"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFB37_11485"
     CDS             <1..287
                     /locus_tag="VFB37_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO23113.1"
                     /translation="GFTEEDEVIERANATEYGLSAGVFTSDLTRAHRVIARLQAGTCW
                     INHYNITPIELPFGGMKMSGLGRENGRAAIEHYTQLKSVYVALTGIESPY"
     gene            300..1961
                     /gene="betA"
                     /locus_tag="VFB37_11490"
     CDS             300..1961
                     /gene="betA"
                     /locus_tag="VFB37_11490"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017429343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HZO23114.1"
                     /translation="MPQSTFDYVIVGAGSAGCVLADRLSADGSSKVLVLEAGGSDRSI
                     FIQMPSALSIPMNMPRYNWFYHTEPEPQLGGRRLHTPRGKVLGGSSSINGLVYVRGNP
                     LDFEQWEAGGARGWGYRDVLPYFRRAEKRAAGEDLYRGGSGKLHTSRGTLANPLNAAW
                     LEAGRQAGYPLTADMNGFQQEGFGAMDMTVGGGRRCSAARAYLRPAMRRSNVSVQAHA
                     LVTRILFEGRRAVGVSYLRGGTEHVARASREVIVSSGAINSPQLLKLSGVGPAPELRA
                     LNIPVVHELPGVGENLQDHLEFYFQVACREPITLYKYQNAWGRALIGARWLLLHDGLG
                     ATNHFETGGFIRSRAGIRFPDIQFHFLPMAVSYDGSSLAREHGFQAHVGPMRSKSRGW
                     VRLATANAEDKPKVFFNYMSHPDDWTEMHACVRLTREIFAQSAFDRFRAREIQPGAGV
                     QTDEQIDAFVREHVESAYHPSCTCKMGADTDPMAVVDRHLAVRGLEGLRVVDSSVMPS
                     ITTGNLNAPTIMLAEKAADHILDRPLLPREDAPFYVAPDWERRQR"
     gene            2094..3170
                     /locus_tag="VFB37_11495"
     CDS             2094..3170
                     /locus_tag="VFB37_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZO23115.1"
                     /translation="MCALGGCGHDPHSGTPAEEKVLNIYNWADYIGRDTITAFERRTG
                     IQVVYDTYDSDETLEAKMMAGDSGYDIVSTSTDYFSRQIKAGIYQALDRKRLPNWKNL
                     DPQVLTEEAAADPGNRHAVPYLRHVNGFAYNVDLVRARMPDAPLDSLDMLFKPQVIAH
                     FADCGVTFLDSAEDVLQLALNYLHLDPNTTRPQDYKRAEQLILAVRPYVRTFDSTEYM
                     NGLADQEFCISMSWSGDYATIRARARAAGADVHLAFTVPREGANGSFDALLIPAGAPH
                     PNAAYEFLNYILEPRVIAQITNDIHYANDNLASRPYVDPEILNDPAVYPTPEMQARLY
                     HSAEVSPALERLRTRTWTRIKTAR"
     gene            3212..4336
                     /locus_tag="VFB37_11500"
     CDS             3212..4336
                     /locus_tag="VFB37_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZO23116.1"
                     /translation="MSGLQITSPTELSIELTGGPRFAIHPLWLRERCRDAKTIDLHTG
                     QRWQDPSDWDPQLALTSVAEVEPDRYRVRFSDGHEADFIGREILAEAALPPGDHDCPV
                     MRLWDGSLEPLPRVPWEDSPAEEVRLGWLEHFLELGFIIFTGVPTQPEMVLRVASRFG
                     YVRDTNFGPCFDVRSTPDAIDLAYTSIALDPHTDNPYRSPVPGVQLLHCLINETSGGL
                     STLVDGFAAAQFLREHDRSAFDRLVRTPVRFRFRDASSEFIASAPLIEADGVGRLQSI
                     NFSPRLDYVPLQSPQELAAFFSSRRALDQLLRSPRFEIRFLLNSGDLLMMDNRRLLHG
                     RTRFDPAEGLRHLQGCYIDIDGPRSQYRVLRRRVASGRTA"
     gene            4333..4920
                     /locus_tag="VFB37_11505"
     CDS             4333..4920
                     /locus_tag="VFB37_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO23117.1"
                     /translation="MSTQPRIVSFRRMEDGTREDYLLLEESEKAYAAGLPGRVLEAVR
                     KLNHSLAGYPVSRLGHSLQAATRALNDGADEELIVAALVHDVGDELAPFNHSEIAAGI
                     LRPYVRPEVTWIVEQHGLVQTYYYVHHTGGDRHMRERLRGHKWFDACLQFCHRWDQSS
                     FDPDYPSKPLEFFEPYLQRIFSRRAHDPRYTKVEP"
     gene            complement(4932..5765)
                     /locus_tag="VFB37_11510"
     CDS             complement(4932..5765)
                     /locus_tag="VFB37_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO23118.1"
                     /translation="MERRFSPVLFCALSFGIAVLYIPILVLIAYSFNASPLVSLWGGF
                     TTEWYTKLIHNRQVLEAALLSLEVAVCASTVAVMLGTLAAISLVRFTRFPGRLLLTGM
                     VNAPLVMPEVITGITQLLLFVSMLQMLSWPHRGFTTIVIAHVAFCSAYVTVTVQSRLQ
                     TADRSLEEAAMDLGSSPARAFLEITLPIIAPALISSWLLSFTLSLDDLVISSFVSGPG
                     ASTLPMVIYSKVKLGVSPDVNALASLIICLVGGCVIGAGILMRRAERQRLLEVQQAER
                     G"
     gene            complement(5771..6661)
                     /locus_tag="VFB37_11515"
     CDS             complement(5771..6661)
                     /locus_tag="VFB37_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO23119.1"
                     /translation="MRLIERPGRWLIILPPLLFLIVFFLIPFAFAFKISLAETAPRVP
                     PFTDILTHTADHHWHLSISLGNYRYLLTDEVYGYAYLYSLRTAFFSTLICLALGYPMA
                     YAISRASKSTQSILLLIIILPFWTSFLLRVYALEGIIRDNGIINILLLRWGIISHPIQ
                     IMRTSLAVYLGIIYSYLPFMVLPLFATLEKLDGTLLEAAADLGSPPWRAFLDVTLPQS
                     LPGVIAGSMLVFIPAVGEFVIPSLLGGPDNLMIGRVLWDEFFGNHDWPVASAVAIAFL
                     FLLAGPIALYQYYHVKQLEA"
     gene            6768..8312
                     /locus_tag="VFB37_11520"
     CDS             6768..8312
                     /locus_tag="VFB37_11520"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZO23120.1"
                     /translation="MPSRGNSNSGPSPVPERASRARQRQRLIDACISALHIHGPSRTT
                     VEKVVALADMSPGIVRFYFDSKDAMLVASLAYLAAEFEERVMQPVARLKETPVRALEQ
                     LVELYLDPDLASPRKVSVWYSFWGEASSRQEYYEICGKKDDDFAALVRELIERLVVQS
                     GDVHLDPDAVALGLIGVLEVLWQSIAFTNEEQIDRAAAKARSLAYLRSIFPRQFGGAA
                     STAQSATRNPPWVYADPMLLNIERERLFRPSWQLAGHESELARAGDYLTLDFTGERAV
                     LVRGSDGEIRAFRNSCRKRPHALLAGARGHLNSEIRCDIHDLRYGLDGKFLAGSTRGD
                     LTALQLAAPGGCLLIRAFGAASAPPGVEWNSLRGFQPSAAQEIDVAADWKLLVEQWLE
                     YQPPAKGVQRVFLAPNHLLELRSAAATLLQVLPVAAGQCRLRRLEYVRAAAGKGKQAA
                     APMRSAKLAGRLRAEIEVAESMQAAILAGAAESDEAVAPGPALADFRRSIASLLPGLE
                     TATRTK"
     gene            complement(8370..9536)
                     /locus_tag="VFB37_11525"
     CDS             complement(8370..9536)
                     /locus_tag="VFB37_11525"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZO23121.1"
                     /translation="MILPAPHTPEPISSERPPHALPAWAYSHPEMTRLEFERILKPSW
                     QIIGHVSSIPRPGDYLTFDFGPESLVAIRTSQGEIRVFHNVCRHRGARILEGVGQCPG
                     AITCPYHGWSYRLTGELIGMPVRESFPGLERSQYSLKPVRMTILCGFVFACLGGDPPA
                     LESIWGHFLEDFAPHRFETLEPLGPLYLEHWDVDWKIAMDNYLESYHVPIGHPGLFRM
                     FTPDYDDQANLPGVARGISWLRERPSSRWSERMYQQLIGQVTRELPEPDRRRWTFYSM
                     LPNLGIDVFPDQMDFFQVLPRGPGKCTIRGGSFARPDSRREMKVVRYLSARINRQVQR
                     EDEFLCGRVQRGLASSSYEPGPLSKLEGCMLEFHDLLRERIPEVRLPSPPARFA"
     gene            9661..10989
                     /locus_tag="VFB37_11530"
     CDS             9661..10989
                     /locus_tag="VFB37_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZO23122.1"
                     /translation="MSRTLTKSHEHFRRAVQHLPLGVASNFRYWGEDRTIYIKQGRGA
                     RVVDLDDNLYIDYRMGYGPAILGYADPRVDAAAREGMLVGGVFALSTERELAVAERIS
                     KMVPAAELVRFSNSGTEAVMAALRLARAYTGRDDYIILEGSYHGLFDAAMWYTPMDKW
                     SQVGDPQVMPYSEGIPVSTRSFAHFVQANDATQLEDVFRRRAGRIACLLIEPIMGNCL
                     GIAADPEYLRAARALCDQYGVVLIIDEVKTGFRVARGGVQELYGVQADLCTFAKALAN
                     GYPISVLAGREKIMRKIGRGVAHGGTYTAHAVSLAAAEKTLEILDETDALERIAQYGT
                     RLRAGMSTALKSRGIAHSFVGHPSMSGLYFAHEPPRNYRDWKGSDYSFYDATARVLHD
                     ERILCEPDSREPWFISAAHDESCLTETLAAFEVAIDTTLRTAGSTRKMPA"
     gene            11018..12622
                     /locus_tag="VFB37_11535"
     CDS             11018..12622
                     /locus_tag="VFB37_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO23123.1"
                     /translation="MVTTSSKRHDVIVVGAGHNGLVAACYLARAGLDVLVLERNPYIG
                     GAAVSRQLYENFTYSNCSYVCSLLRTEIIRALELPRFGLQIIPYHGGCTLMRDGGHLA
                     IYDNHDALRREFARHSKRDAEAYDRYARDMMRHCRFIKPLLLREPPDPTSFKPRDLME
                     LLHLGRHFQQLGEMQMYDTLRFWTMSCADLLDEYFESGIIKAHLAGSSIIGTALGPRS
                     PGSAYVLLHHYMGSIDGTVGAWGFARGGMGAITAALAASLQASGGSIRSEAPVERILV
                     RNGRATGVALASGEEIPARIVVSNLDVRRTFLKTMDAADLPADFLKLVRNFKIRGSSG
                     KLNIALDGLPNFPAIPAGAPCTRGDMHVTDTIDMLERAYDDWKEGHWSRAPYLDMLIP
                     SRIDPTMAPDGKHYMSVFVQYCPYELAQGPWNDSTRKAFGNTVIESIARHSPNFKDLI
                     LHAEIRTPWDLENEVGLTEGNIFQGELTFDQLLFNRPVPGYAQYRAPVRGMYMCGSST
                     HPGGGVMGAPGFNAAGAILRDAGRTM"
     gene            12619..14244
                     /locus_tag="VFB37_11540"
     CDS             12619..14244
                     /locus_tag="VFB37_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO23124.1"
                     /translation="MSSTTTARNAAVPAGRSHYDCVVIGGGHNGLVCAAYLARKGRSV
                     CVLEAAGEVGGAAVTREFAPGFSVSACAHLLHSMSAQVCRELQLERHGLQWAATRMPT
                     AALSEEGTALTLTGDTARALHESACSATDAAAYPAYAARLVRFARALSPVLESVPPRL
                     GTDSWQDRRALLRLGWQIRRLGRSDMRELLRIGGMSVYDLLKDNFTSPLLQGALAFDA
                     VLGTNLGPRSPGSVFTLLYRLATVAGAGADQYGGVLSQPVGGLGRLSQALAGAAEAAG
                     AQVRKSAPVERILVRHDRAVGVTLSSGEDIEAEAIISNVDPKSTFLRLLGIEHLDAGF
                     VRRIAHLRTQGLAAKLHLALDELPKFRGLPPEALSGRVLLAPSLDYLERAYNHSKYRE
                     VSASPALEITVPTVNDPGLAPQGCHVLSAVVQYAPYTLDKGWEGERRVFTERLIDLLD
                     SCAPGLRRSVRAAQLLAPPDLEREFRITGGHWHHTDLALDQSFMVRPVPGAAQYRTPL
                     EGLYLCGAGCHPGGGVMGAAGRNAAQQVLARVA"
     gene            14255..15451
                     /locus_tag="VFB37_11545"
     CDS             14255..15451
                     /locus_tag="VFB37_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HZO23125.1"
                     /translation="MHFSEPLLKTPFHDRARRESQLDSFVPWSGYTTVDVFTQVEEEY
                     FAIRNAASVYDLTPMIKYRITGPGALAYLNRLVTRDLRKLKVARVAYCVWCDDAGNVI
                     DDGTVFRLGENEYRLCTAYRQLDWLLASAIGFEGAAAVRIEEVTDEIAALSLQGPTSF
                     AVLKTLGLRGVERLRPFEIGFCALDDIPLTISRTGFTGDLGYELWMSPAHAVAVWDAL
                     MAAGRSRGIRPIGSRALNIARIEAGFLQPNLDFISSAHTQRPGRDRTPLELGLEWLVD
                     FDKGHFTGRRALLAQRRQGLKRRLAGLDIEGNKPAHNALLYAERSGQRQIGSVSSASW
                     SPTCKRNIALAMLDAPHFESGRGLWAEIYLNQELVWERRMVRARVVDRPFFNPERRKL
                     TPPRDF"
     gene            15507..16628
                     /locus_tag="VFB37_11550"
     CDS             15507..16628
                     /locus_tag="VFB37_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO23126.1"
                     /translation="MAERPWLDPTATPQIVIRGVSKIYGAFTAVDDINLTIFKGEMFA
                     LVGASGCGKTTLLRMLAGFADQSAGQITIDGVEMAGVPPHERPVNMMFQSYALFPHMS
                     VERNVGYGLRRLDLARAEKERRVQEALEMVQLGHLRARKPHQLSGGQRQRVALARALI
                     RRPKVLLLDEPLSALDKKLREKTQFELMDLQHQVGITFIVVTHDQDEAMALATRIAVM
                     DRGRIVQVGTPSEIYEFPQSRFVADFVGTTNLFEGTVAACEPGLIKVRCKETGCDLLV
                     DDVGTFAPGQQVWVALRPEKIRLSKQPASPGTINQLHGTVWELGYLGNRSTYQIRTSS
                     GKLITVFAQNERRTSQWSIDWSDEVYVTWQADAAVILQA"
     gene            complement(16642..19317)
                     /locus_tag="VFB37_11555"
     CDS             complement(16642..19317)
                     /locus_tag="VFB37_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO23127.1"
                     /translation="MNSKVSSAIAAILSGSSIGAAYAVEPADTSAAPSDAIQEVVVTA
                     QRRSENMQDVPISIQAMTSETLQQLNVATFDDYIKYLPNVTTANNGPGQNEIFMRGLS
                     AGSQPSQGSASTGLWPNVAIYLDNQSGQLPNRNLDVYAADLNRIEVLEGPQGTLFGAG
                     AEAGVIRYITNEPKLDTTEANIKAGYGATAHGDPNTDVTAVLNLPLIADHMALRAVIY
                     DDRRGGYIDNVPATFTRKNTDIGIHYANFPAVNGQCPDGLPNNGFCVPPGSPSINNSS
                     IASRAINPVTYKGTRVEALYKFNDDWDALITQSYQQMDAEGVFYQQPNASDGAPLRPL
                     EVTLFNNAFDTDKFESTAWTLNGKIAFLKAVYTGGYLVRNVQQVGDYTNYARGVYADY
                     YQCFGPGSGGEANLKPTCFSPSAVWRSSERNTHQQHEFRLSTPDEWRFRGIAGVFWEN
                     NTLFDETGWGYKSVPSCTANGPAGSPGNSGCFADIGTAPGATVVFPGVHPAPTSFYQD
                     TVRQTKQTAFFASVDFDIIPKVLTVTAGTRHFRFNNSSDGSVTGSFGCFDGGAPTVGC
                     QAEAFNLNGANLRDTESGFKSRGNLTWHLSPDLMLYYTYSQGFRPGGFNQNGGAAHAP
                     GTDGEAQFIIPSAYHSDKLTNNEIGWKSEFLDHRLQWNGAIYRENWDDVQVAFFDPGV
                     VGNIFFNTNGQNFLIKGVETQLVARLVTGLTLQGSASWNQSRQTNSPLLIDNNPASAN
                     FGKPISQTCDSTGANCAPITNPFGPVGSPSADAPPLQFALRARYEWVIGSYTPYVQLG
                     ATHSGHSFTQAGSNPTFAEAGAVSTGRLRFENPAYTTYDASVGLAKDAWYADLYIENL
                     SNSNASTFVSTDQFIIAQTPLRPRVIGLTFGYKML"
     gene            complement(19422..20420)
                     /locus_tag="VFB37_11560"
     CDS             complement(19422..20420)
                     /locus_tag="VFB37_11560"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZO23128.1"
                     /translation="MPLRVGIILADHFTLSAFAVFIDHLRLAADEGDRSRPLHVQWSI
                     MASRESSVPASCGVLIEPTSNFLEPESLDYVVVVGGILHAGPQVDEATLRYLRDVAAS
                     TTPLIGICTGSFILCRAGLMKGRRSCVSWYHYQDFLEAFPGQEVVADRLFLADGPRIT
                     CAGGAGTAALATFLIEQHLGRSVAQKASQVLLFDRARSGSDTQPHPPLSESVREPRVR
                     RALLLMEQNLTRPVAIATVADELGVSVRQLERLCREHVGMGPASLYRQLRMRYARWLV
                     ENTDRSVTEIAIEAGFADCAHFSRQFKDAYGLSPSTRRLQPNRTTYGDAARARVFE"
     gene            complement(20678..21244)
                     /locus_tag="VFB37_11565"
     CDS             complement(20678..21244)
                     /locus_tag="VFB37_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23129.1"
                     /translation="MSNDSNRITALTPDPKPWPEPSSSRMLTTYLADVEQLLSEQHWE
                     MALRDAIDLPRIAVALTDPQMCVSSERCKAWCEEWIRPANEDEHAAGMDHGDLCRVLS
                     EHVATDLEAANEGVPSTALRRLRLRRHARNAPRGFNIEMPDTQDPEAADAVQICTAVV
                     EGVRRWYAHSACHDAVAQANLARLAVLR"
     gene            complement(21309..21653)
                     /locus_tag="VFB37_11570"
     CDS             complement(21309..21653)
                     /locus_tag="VFB37_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23130.1"
                     /translation="MDPLERVRLQAEVAKQRVRIAKDELKRARKRLKEAKREARNARK
                     LASSSRKEWKRARRRAKRNDSIVIAQTAARAVPDKETMSTGRPKRSRGVPRRASGSAT
                     AAVRRKRRRTQR"
     gene            complement(21970..>23034)
                     /locus_tag="VFB37_11575"
     CDS             complement(21970..>23034)
                     /locus_tag="VFB37_11575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO23131.1"
                     /translation="GGQTTVIQRILERRAQTPPAIQEDTSATIAVMLRVLPPPRALLV
                     FEDSALLSHLQRRIASELLDGEAITEEQEAVRRFAAEFYPVILTDNPELIRKIRARKS
                     ERTPYILYVAELDEASERDAGLLAGADDCIGRRAPEREMHARVSAARRIAELEAVLRI
                     ALVENRKLSTTDDLTRVASRRFFTKHFPREVERAARYGRALSLILCDIDHFKNVNDTL
                     GHAAGDEILRQFGARLQDALRRGVDWVARIGGEEFAVVLPETGYEQALDVARKLRATI
                     ANKTFETSARSVEVTASLGLCGIERVPGGEKRIAESLVKVADAALYRSKNAGRNRVTA
                     TRLPASKGERSTARRPRGAR"
BASE COUNT         4092 a         7297 c         7518 g         4127 t
ORIGIN      
        1 tcggcttcac tgaggaagac gaggttatcg agcgggcgaa cgccaccgag tatggcttgt
       61 ctgccggggt cttcaccagc gatctgaccc gcgcgcaccg ggtgattgca cggctgcagg
      121 ccggcacctg ctggatcaat cactacaaca tcacgcccat cgagctgccg ttcggcggga
      181 tgaaaatgtc gggtctcggg cgcgagaacg gtcgcgcggc catcgagcac tacacgcagc
      241 tcaagagcgt ctacgtcgcc ctgaccggca tcgagtcccc ctactgacgg gctgcgcagt
      301 tgccgcaatc cacattcgat tacgtgatcg tcggggcggg ctcggccggc tgcgtgctgg
      361 cggaccggct cagcgcggac ggcagcagca aggttctggt gctggaagcg ggcggctcgg
      421 atcgctcgat cttcatccag atgccctccg cgctgtcgat tcccatgaac atgccgcggt
      481 acaactggtt ctatcacacc gagccggagc cgcaactcgg gggacggcga ctgcacaccc
      541 cgcgcggaaa ggtgctcggc ggttcctcat cgatcaatgg actggtctat gtacgcggca
      601 atcctctgga cttcgagcag tgggaggcgg gcggggcgcg cggctggggc tatcgggacg
      661 tgttgcctta cttccggcgt gccgagaagc gtgccgccgg cgaggatctc tatcgaggcg
      721 gctccgggaa gctgcacacc tcgcgcggca ctttggcgaa ccccctgaac gcggcatggc
      781 tcgaggccgg gcgacaggcg ggctatcccc tgaccgccga catgaatggc ttccagcagg
      841 aaggattcgg ggccatggat atgaccgtgg ggggcggcag gcgctgcagt gccgctcggg
      901 cctatctgcg gcccgccatg cgcaggagca atgtgtcggt gcaggcgcac gcgctcgtca
      961 cgcgtattct cttcgaggga cgccgggcgg tgggagtcag ctaccttcgg ggcggcacag
     1021 aacacgtggc ccgcgcgagc cgcgaagtga tcgtgagctc aggggcgatc aactccccac
     1081 agctgctgaa gctctcaggc gtcggtcccg caccggagct ccgggcactg aatatccccg
     1141 tggtgcacga gctgccgggg gtgggtgaga acctgcagga tcacctcgag ttctatttcc
     1201 aggtcgcctg tcgcgagccg atcacgctct acaagtatca gaacgcctgg ggtcgggcac
     1261 tgattggcgc gcgctggctg ctgctgcacg atggcctcgg cgccaccaat catttcgaga
     1321 cgggcgggtt cattcgcagc cgtgcgggaa tccgcttccc ggacatccag ttccatttcc
     1381 tccccatggc ggtgagctac gacggctcct cgcttgcacg cgagcacggt tttcaggctc
     1441 atgtggggcc catgcgctcc aagagcagag gctgggtgcg gctcgcgacg gccaacgctg
     1501 aggacaagcc gaaggtcttc ttcaactaca tgtcgcatcc ggatgactgg accgagatgc
     1561 acgcctgcgt gcgtctgacc cgtgagatat tcgcgcagag tgccttcgac cgatttcgcg
     1621 cccgtgagat ccaacccggc gcgggcgtgc agaccgatga gcagatcgat gctttcgtgc
     1681 gcgagcatgt cgagagcgcc tatcacccct cgtgtacgtg caagatgggc gcagacaccg
     1741 atccgatggc ggtagtggac aggcatctcg cggttcgagg cctggaggga ctgcgggtgg
     1801 tggattcctc ggtgatgccc agtatcacca caggcaacct caacgcaccg accatcatgc
     1861 tggccgagaa ggccgcggat cacatcctgg acagacccct gctgccgcgt gaggacgcac
     1921 ctttctacgt cgcacccgac tgggagcgcc gccaacgttg agctcctgcc tgtacgcact
     1981 tgcgccgttc ttattatggc gtcaataata actggggcgg acagcgggag ctgccggaga
     2041 tggcggcggc gagatcgatg agggcgcgag cggcgtgcgg aattttggct gcagtgtgcg
     2101 ctctgggcgg ctgcggccac gacccgcact cgggcactcc tgccgaagag aaggtgctga
     2161 acatctataa ctgggccgat tacatcggcc gggacaccat cacggctttc gagcgccgca
     2221 ctggcatcca ggtcgtctac gacacgtacg actccgatga gacgctcgag gccaaaatga
     2281 tggccgggga ctcgggctac gacatcgtga gcacctccac cgactatttc agccgccaga
     2341 tcaaggccgg gatctaccag gcgctcgacc gcaagcgcct gccgaactgg aagaacctcg
     2401 atccgcaggt gctcaccgag gaagccgcgg ccgatcccgg caatcgccac gcggtgccct
     2461 atctgcgcca cgtcaatgga tttgcgtaca acgtggacct ggtccgagcg cgcatgcccg
     2521 atgcgccgct cgacagcctc gacatgttgt tcaagccgca ggtgatcgcg catttcgccg
     2581 actgcggggt caccttcctc gactctgccg aggacgtact gcaacttgcg ctcaactatc
     2641 ttcacctgga ccccaacacg acgcggccgc aagactacaa gcgcgccgag cagctcattc
     2701 tggcagtccg accttacgtg cggacgttcg actccaccga gtacatgaat ggcctggccg
     2761 atcaggaatt ttgcatctcg atgtcctggt cgggtgacta tgcgaccatc cgagccaggg
     2821 cgcgcgccgc cggtgccgat gtgcatctcg ccttcacggt tccgcgggag ggcgccaacg
     2881 gctcgttcga tgcgctgctg attccggccg gcgctccgca tccgaacgcg gcgtatgagt
     2941 tcctgaacta tatcctcgag ccgcgcgtca ttgcgcagat caccaacgac attcattatg
     3001 cgaatgacaa cctcgcttcg cgcccgtacg tcgatcccga gatcctgaac gaccccgccg
     3061 tctatccgac tcccgagatg caggcgcggc tgtatcactc ggcggaggtc tcaccggcac
     3121 tcgagaggct tcgcacccgc acatggacgc gtatcaagac ggcaaggtaa gtccgcagcg
     3181 tcgggcgaat cctgtgccag gagaaatgag catgtcaggc ctgcagatca cctcaccgac
     3241 cgagctgagt atcgagctca ccggtggccc gcgcttcgcc atccatccgt tgtggctgcg
     3301 ggagcgatgc cgggacgcca agaccatcga tctgcatacg gggcagcgct ggcaggaccc
     3361 ctccgactgg gatccgcagc tcgcgctcac ctcggtcgcc gaagtcgagc cggatcgcta
     3421 ccgcgtccgc ttcagcgatg gtcacgaggc cgacttcatc ggccgcgaga tccttgccga
     3481 agcagcactc cccccgggcg accacgactg cccggtgatg cggctgtggg atggctctct
     3541 ggaaccgctc ccgcgcgtgc cctgggagga ctcgccggca gaggaggtgc gattgggctg
     3601 gctcgagcac ttcctcgagc tcgggttcat catcttcacc ggcgtgccca cgcagccgga
     3661 gatggtcctg cgtgtagcgt cccgcttcgg ttacgtacgg gatacgaact tcggtccctg
     3721 cttcgatgtg cgctcgaccc cggatgccat cgacctcgcc tacacgagca tcgcactcga
     3781 tccgcacacc gataacccgt accgctcacc ggtacccggc gtgcagctgc tgcactgcct
     3841 gatcaacgag acctcgggtg gcctctccac gctggtggac gggttcgccg ccgcacaatt
     3901 cctgcgtgag cacgatcgct cggccttcga ccggctggtc cgtacgccgg tgcgctttcg
     3961 attccgcgat gcgagctcgg agttcatcgc cagcgccccg ctgatcgaag cggacggcgt
     4021 tggacgcctg cagtcgatca acttcagccc gcgtctggac tacgtgccgc tgcaatcccc
     4081 gcaggagctc gcggcattct tcagctcgcg ccgcgcgctc gatcagctgc tgcgctcgcc
     4141 gcgtttcgag atccgcttcc tgctcaattc cggcgatctg ctcatgatgg acaatcgccg
     4201 gctgctgcat gggcggaccc gcttcgaccc cgccgagggc ctgcggcatc tgcaaggctg
     4261 ttacatcgac atcgatggac cgcgcagcca gtatcgcgtt ctgcgccgtc gggtggccag
     4321 cgggaggacg gcatgagcac gcagccgcgc atcgtcagct ttcgccgcat ggaggacggc
     4381 acgcgcgaag actatctgct tctggaggaa tcggagaaag cctatgccgc cggtctgccg
     4441 ggacgcgtgc tcgaggccgt gcgcaagctc aatcactccc tcgccggtta tccggtcagc
     4501 aggttgggcc actcactgca agcggccacc cgcgcactca acgacggagc cgatgaagag
     4561 ctgatcgtcg cagcactcgt gcacgatgtc ggcgacgagc tggcgccttt caaccattcg
     4621 gagatcgccg cggggattct ccggccctat gtccggccgg aggtcacctg gatagtggag
     4681 cagcacgggc tggttcagac ctattattac gttcaccaca ccggaggcga tcgtcacatg
     4741 cgcgagcgcc tgaggggcca caagtggttt gatgcctgtc tgcagttctg tcatcgctgg
     4801 gatcaatcct ccttcgatcc cgactatccg agcaagccgt tggaattttt cgagccctac
     4861 ctacagcgta tcttcagccg ccgcgcacac gatccgcgtt acacgaaagt cgagccgtag
     4921 cgcccggctg actagccccg ctcggcttgc tggacctcca gcaggcgctg gcgctcggcg
     4981 cggcgcatga gaatgccggc cccgatcacg catccgccga ccaggcagat gatcaggctc
     5041 gcgagcgcgt tcacatcggg gctcaccccg agcttcactt tcgagtagat gaccatcggc
     5101 agcgtgctgg cgccggggcc ggacacgaag ctcgagatca cgagatcgtc gagtgataac
     5161 gtgaagctca gcagccagct cgagatcagc gccggtgcaa tgatcggcag agtgatctcg
     5221 aggaacgccc gggccgggct cgatccgagg tccatggccg cttcctcgag ggagcggtct
     5281 gcggtctgca gccgcgattg cacggtgacc gtgacgtatg cactgcagaa ggccacatgc
     5341 gcgatgacga tcgtcgtgaa tccccggtgc ggccaggaaa gcatctgcag catcgagacg
     5401 aacagcagca gttgagtgat gcctgtgatg acctcgggca tgacgagcgg tgcgttcacc
     5461 attcccgtca gcagcagtcg tccggggaaa cgggtgaagc gcaccaggga gatggcggcc
     5521 agggttccga gcatgacggc cacggtcgat gcgcagacgg cgacctcgag agacaggagg
     5581 gccgcctcga gcacttgccg gttgtgaatc agcttcgtgt accactcggt ggtgaagccg
     5641 ccccaaagac tgaccagggg cgaggcgttg aacgaatacg cgatcagtac caggatgggg
     5701 atgtacagca ccgcgatgcc gaaactcagc gcgcagaaca gtaccgggga aaagcgccgc
     5761 tccatcgcct tcaggcctcg agctgcttca cgtggtaata ctggtagagc gcgatcggtc
     5821 cggcgagcag gaacagaaac gcaatcgcga ccgccgaggc aaccggccag tcatggttgc
     5881 cgaaaaactc atcccacagc acgcgcccga tcatcagatt atcgggacct ccgagcagcg
     5941 acgggatcac gaactcgccg accgccggga tgaacacgag catggagccc gcaatgactc
     6001 ccggcaggga ttgcggcaaa gtgacatcga ggaacgcgcg ccagggggga ctgccgagat
     6061 ccgccgcggc ctcgagcagc gtgccgtcga gtttctcgag cgtggcaaac aacggcagca
     6121 ccatgaaggg caggtaggag taaatgatac ccagatagac cgccaggctc gtgcgcatga
     6181 tctggatggg atgggagatg attccccagc gcagcagcag gatgttgatg atgccgttgt
     6241 cccggatgat gccctcgagc gcatacacgc gcaaaagaaa ggacgtccag aagggcaata
     6301 tgatgatcag cagcaggatg ctctgggtcg atttcgaagc ccgcgagatc gcataggcca
     6361 ttgggtagcc cagcgccagg cagatgaggg tggagaagaa ggcggtccgc aacgagtaca
     6421 ggtacgcgta cccatacacc tcatcggtga gcagataacg gtagttgccg aggctgatcg
     6481 acagatgcca atgatggtct gccgtgtgcg tcaggatgtc cgtgaacggt ggaacgcgcg
     6541 gggcggtctc ggccaggctg atcttgaagg cgaaggcaaa cgggatgaga aagaagacga
     6601 tcaggaatag cagcgggggc aggatgatga gccagcggcc cgggcgctcg atcaatcgca
     6661 tgttcggtgc gtgcagggtg tctgtcggct attctgcgcg ataaactccc aggcgtgtac
     6721 aagttttgcc cggtccgcac cgccgggctc gccagaggcc caccatcttg cccagtcgcg
     6781 gaaactccaa cagcgggccc tctcccgtcc ccgagcgcgc ctcgcgggcc cgtcagcgtc
     6841 aacggctgat cgatgcgtgc atctcggccc tgcacattca cggcccatcg cgcacgacag
     6901 tcgagaaagt cgtggccttg gcggacatgt ccccggggat cgtgcggttc tacttcgatt
     6961 ccaaggatgc aatgctggtg gcctcactgg cttacctggc cgcggaattc gaggagcggg
     7021 tcatgcagcc ggtggcgcgg ctgaaggaga cgccggttcg cgcgctcgag cagctcgtcg
     7081 agctgtatct cgacccggat ctcgccagcc cccggaaggt ctcggtgtgg tactcgttct
     7141 ggggcgaggc cagctcgcgt caggagtact acgagatctg cggcaagaag gatgatgact
     7201 tcgccgccct ggtgcgcgag ctcatcgagc ggctggtcgt tcagagcggg gatgtacacc
     7261 tggatcccga tgcggtggcg ctggggctga tcggcgtcct cgaggtgctc tggcaaagca
     7321 tcgcgttcac gaatgaagag cagatcgacc gcgccgctgc gaaggcgcgc tcgctcgcat
     7381 atctgcgatc gatcttcccc cgccagttcg gcggggcggc ctccacggcc caatcggcaa
     7441 cccgcaatcc gccgtgggtg tatgccgatc ccatgcttct gaacatcgag cgcgagaggc
     7501 tgttccggcc ttcctggcag ctcgcaggtc atgagagcga gctcgcccgg gcgggtgatt
     7561 acctgacact cgacttcacg ggtgagcgcg ccgttctcgt gcgcggcagt gatggcgaga
     7621 ttcgtgcgtt ccgcaacagt tgccgcaagc ggccgcacgc tcttctcgcc ggcgcgcgcg
     7681 gccatctcaa ctccgagatc cgctgtgaca ttcatgacct gcgctacggg cttgacggga
     7741 agttcctcgc aggcagcacg cgcggcgacc tgacagcctt gcagctcgcg gcgccgggcg
     7801 gatgcctgct gatacgcgcc ttcggggccg cctcggcgcc tcccggtgtt gagtggaact
     7861 ccctgcgggg ctttcagccc tcggcggcgc aggagatcga tgtggccgca gactggaagc
     7921 tgctggtcga gcagtggctc gagtaccaac ctccggccaa aggcgtgcaa cgtgtgtttc
     7981 tggcgcccaa tcatctgctc gagctgcgat ccgccgccgc gacgctgctc caggtgctgc
     8041 cggtcgccgc aggccaatgc cggctgcgac gcctggagta tgtgcgcgca gcggctggca
     8101 aaggcaaaca ggcggcggct ccgatgcgat cggccaaatt ggcggggcgg ttgcgcgcag
     8161 aaatcgaggt cgccgaatcc atgcaggcgg caatccttgc gggcgcggca gaaagcgacg
     8221 aggcggtggc cccgggtccg gcactcgcgg acttccggcg cagcattgcc tcccttctgc
     8281 ccggtctcga gacggccacc cggaccaaat agttgaccac aagttgatac cggctccggc
     8341 tgcgccccgg gcgagcgcgc gccagccact caggcgaaac gtgccggcgg gctcggcagg
     8401 cgcacctccg ggattcgctc gcgcagcagg tcatggaact cgagcatgca gccttcgagc
     8461 ttcgagagtg ggccgggctc gtagctgctc gaggcgagcc cccgctgcac gcgcccgcac
     8521 aggaactcgt cctcgcgctg gacctggcga ttgatccgcg ctgacaggta gcgcaccact
     8581 ttcatctcgc gccggctgtc ggggcgtgcg aaagagcctc cacgaatggt gcacttgccg
     8641 ggaccacggg gcaggacctg gaagaaatcc atctggtcgg gaaacacatc gatgccgaga
     8701 ttgggcagca tgctgtaaaa cgtccagcgc cgccgatccg gctccggcag ctcgcgggtg
     8761 acctggccga tgagttgctg atacatgcgc tcgctccagc ggctcgaagg ccgctcgcgc
     8821 aaccagctga tgccgcgtgc cacaccgggc aggttggcct gatcgtcata gtcgggtgtg
     8881 aacatccgaa acaaccccgg gtgtccgatc ggcacgtggt acgactccag gtagttgtcc
     8941 atggcgatct tccaatccac atcccagtgc tccagataca gaggccctag cggctcgagc
     9001 gtctcgaacc gatgcggcgc gaagtcctcg aggaagtggc cccagatgct ctcgagcgca
     9061 ggcggatcgc cgcccaggca ggcaaacacg aagccgcaca gaatcgtcat gcgcaccggc
     9121 ttcaggctgt actgcgagcg ctccaggccg ggaaaacttt cgcgcacggg catcccgatc
     9181 agctcgcccg tcaatcgata cgaccagccg tgatagggac acgtgatcgc cccgggacac
     9241 tggccgaccc cctccagaat gcgtgccccg cgatggcgac agacgttgtg gaacacccgg
     9301 atctcgccct gcgaagtgcg gatcgccacc aggctctccg ggccgaagtc gaatgtcagg
     9361 tagtcaccgg gacggggaat cgagctgaca tgaccgatga tctgccagct gggcttgagg
     9421 atccgctcga attccagccg cgtcatctcc ggatggctgt acgcccaggc gggcagggcg
     9481 tggggcgggc gctcggaaga aatcggctcg ggcgtgtgcg gggcgggcag gatcatcacg
     9541 ggcgctccgg cagggattta tatagtcatg caagtctagc ccaaaaaact atatgttcat
     9601 atagacgcat gccgtgcccg atggtaccgt gtcgcaagcc catcccgatc gagcgccgcc
     9661 atgtcccgga ccctgaccaa gagccacgag catttccggc gtgccgtcca gcacctgccg
     9721 ctgggtgtcg cctccaattt ccgctattgg ggcgaggatc gcaccatcta catcaagcag
     9781 gggcgtgggg cccgggtcgt ggacctcgat gacaacctct acatcgacta tcggatgggc
     9841 tacgggccgg cgatcctcgg ctatgccgat ccacgggtgg atgcggccgc acgcgagggc
     9901 atgctggtgg gaggcgtgtt tgcgctctcc actgagcggg agctggcagt cgccgagcgg
     9961 atctcgaaga tggtgcccgc ggcagagctg gtgcgctttt caaactccgg gacggaggcc
    10021 gtcatggcgg cgctgcggct cgcgcgcgcg tacacgggcc gggatgacta catcatcctc
    10081 gaaggcagct atcacggctt gttcgacgcc gccatgtggt acaccccgat ggacaaatgg
    10141 agccaggtgg gcgatccgca ggtgatgccc tacagcgagg gcattccggt cagcacgcgc
    10201 agctttgccc atttcgtgca ggccaacgat gccacccagc tggaggatgt cttccggcgc
    10261 cgggctgggc gcatcgcttg cctgctcatc gagcccatca tgggcaactg cctcggaatc
    10321 gccgccgacc ccgagtatct gcgcgccgcc cgcgcgctct gcgaccagta tggggtggtg
    10381 ctgatcatcg acgaagtgaa gaccggcttc cgcgtggccc gcggaggggt gcaggagctg
    10441 tacggcgtgc aggccgatct gtgcacgttc gccaaagcac tggcaaacgg ttatccgatc
    10501 tcggtgctgg cggggcgcga gaaaatcatg agaaagatcg ggcgcggtgt ggcccatggc
    10561 ggcacctaca ccgcccatgc cgtgtcgctc gcggccgccg agaaaactct cgaaattctg
    10621 gatgagaccg acgcgctcga gcggatcgcc cagtatggga cgcggctgcg cgccggcatg
    10681 agcacggcgc tcaagtcaag ggggatcgcg cacagctttg tgggccaccc gtccatgagc
    10741 gggctgtact ttgcgcacga gccgccgcgc aactatcgtg actggaaggg cagcgactac
    10801 tccttctacg atgccacggc ccgggtcctg cacgatgagc ggattctgtg cgagccggat
    10861 tcgcgcgagc cctggttcat cagcgcggcc catgacgagt cgtgtctgac cgaaaccctt
    10921 gcggccttcg aggttgcgat cgacactacc ctgcgcaccg cgggatcgac acgcaagatg
    10981 ccggcgtgat cgcgcctgcc gggggggcgg gcagggcatg gtgactacct cgagcaaacg
    11041 ccacgatgtc atcgtcgtgg gcgcaggaca caacggcctg gtcgccgcgt gctatctggc
    11101 ccgcgcgggc ctcgacgtac tcgtactgga gcgcaatcca tacatcgggg gcgcggcagt
    11161 gagccgccag ctctacgaga acttcaccta ttcgaattgc tcgtacgtgt gcagcctgct
    11221 gcgcaccgag atcatccggg cgctggagct gccgcgcttc ggcctgcaga tcattcccta
    11281 ccacggcggc tgcacgctga tgcgcgatgg cggccatctt gcgatctatg acaatcacga
    11341 tgcgttgcgc cgtgagttcg caagacactc caaacgcgac gcggaggcct acgaccgcta
    11401 tgcgcgcgac atgatgaggc actgccgctt catcaagccg ctgctgctgc gcgagccgcc
    11461 cgatccgaca tccttcaagc cgcgcgatct gatggagctg ctgcatctcg gcaggcattt
    11521 ccagcagctc ggcgagatgc agatgtacga caccctgcgc ttctggacga tgagctgcgc
    11581 ggatcttctg gatgagtact tcgagagtgg catcatcaag gcacacctgg ccggaagctc
    11641 gatcatcggc acggcgctcg ggccgcgctc gccgggaagt gcctacgtgc tgctgcatca
    11701 ttacatgggc agcatcgacg gcacggtggg cgcctgggga tttgcgcgcg gcggcatggg
    11761 agcgattacc gcagcgcttg ccgcctccct ccaggcgagc ggcggcagca tccgtagcga
    11821 agcgcctgtc gagcgcatcc tggtgcgcaa cggccgcgca actggagtcg ccctggccag
    11881 cggcgaggag attcccgctc gcatcgtcgt gtcgaatctg gatgtacgac ggaccttcct
    11941 gaagaccatg gatgcggcgg atctgcccgc ggacttcctc aagctggtgc gcaatttcaa
    12001 gatccgcgga tcctccggca agctcaatat cgcgctcgac ggcctgccga acttccccgc
    12061 gatcccagcg ggagctccct gtacgcgcgg cgacatgcac gtgaccgaca cgatagacat
    12121 gctcgagcgg gcttacgatg actggaagga gggccactgg tcccgggcgc cgtacctgga
    12181 catgctgatt ccctcgcgca tcgaccccac gatggcgccc gatggcaagc actacatgtc
    12241 cgtcttcgtg cagtactgcc cctacgagct cgcgcagggt ccgtggaatg actccacgcg
    12301 caaggccttc ggcaacacgg tgatcgagag cattgcccgg cacagcccta acttcaagga
    12361 tctgatcctg catgccgaga tccgcacgcc ctgggacctg gagaacgagg tgggcctgac
    12421 cgagggcaac atcttccagg gcgagctcac ctttgatcag ctgcttttca atcgccccgt
    12481 gcccggctat gcgcagtacc gcgcaccggt ccggggaatg tacatgtgtg gctcgagtac
    12541 acatccaggc ggtggcgtca tgggcgcgcc cggcttcaat gcggcagggg ccattctgcg
    12601 cgacgcgggc aggacaatgt gagctcgaca accacagcga ggaatgcggc cgtcccggcc
    12661 ggccgctcac actatgactg cgtggtcatc ggtggcgggc acaacggact ggtgtgcgcg
    12721 gcctacctgg cccgcaaggg ccgttccgtc tgtgtgctgg aggcagccgg tgaagtcggt
    12781 ggcgcggcgg tcacgcgcga gttcgcgccc ggcttctcgg tgtccgcttg cgcgcatctg
    12841 ctgcattcga tgtcagctca ggtctgtcgg gagctgcaac tggaacggca tggattgcaa
    12901 tgggctgcga cgcggatgcc caccgcggcg ctttcggagg agggtaccgc acttactctc
    12961 accggcgaca ccgcccgagc gctgcacgag tcagcctgtt ctgccacgga tgcggcggct
    13021 tatccggcct atgcggcgcg cctcgtgcgg tttgcccgag cgctgagccc cgtgctcgag
    13081 agcgtgccgc cccggctcgg aactgattcc tggcaggacc gccgggcact gctgcgcctg
    13141 ggctggcaga ttcgcaggct cggacgatcg gacatgcgcg agctgttacg gatcgggggc
    13201 atgagcgttt acgacctgct caaggacaat ttcacctcgc ctctgttgca gggtgcgctc
    13261 gccttcgatg cggtgctcgg aaccaatctg gggccgcgct cgcctggctc ggtcttcacg
    13321 ctcctgtacc ggctggccac cgtggcagga gcgggcgcgg atcagtacgg cggagtcctg
    13381 agtcagcctg ttggaggact gggcaggctg agccaagcgc tcgcgggcgc ggcggaggcg
    13441 gcaggtgcac aggttcgcaa gagcgccccc gtcgagcgca tcctcgtgag acacgatcgg
    13501 gcggtcgggg tcaccctcag ctcaggagaa gacatcgagg ctgaagccat catctccaac
    13561 gtcgatccga aatcgacgtt cctccggctg ctgggcattg agcacctcga tgccggattc
    13621 gtgcgccgca ttgcgcacct tcgcactcag gggctcgcgg ccaaactgca tcttgcgctg
    13681 gatgagctgc cgaagttccg cggcttgcct cccgaggcgc tctctggccg tgtgctgctg
    13741 gccccgtcgc tggactatct ggagagggcc tacaaccatt cgaagtatcg cgaggtctcc
    13801 gcctcgccag ccctcgagat caccgtgccg acggtgaatg atccgggcct ggctccgcaa
    13861 ggctgtcatg tactctccgc cgtcgtgcag tacgctccat atacgctcga caagggatgg
    13921 gagggggagc ggcgggtgtt taccgagcgg ctcatcgacc tgctggattc ctgcgctccg
    13981 gggctgcggc gctcggtccg cgcggcgcaa cttctcgcgc cccccgatct ggagcgcgag
    14041 ttccgcatta cgggcggaca ctggcatcac acggatcttg ctctcgatca gtccttcatg
    14101 gtgcgtcccg tccccggtgc cgcccagtac cgcacacccc tggaaggcct ttacctgtgc
    14161 ggcgccggct gtcaccccgg aggcggggtg atgggtgcgg cgggacgcaa tgccgcgcag
    14221 caagtgctcg cgcgagtggc ctgaggcctc gcgaatgcat ttcagcgagc cccttctcaa
    14281 gacgcctttc cacgatcggg cgcggcgcga gagccagctc gacagcttcg tgccctggtc
    14341 gggctatacg accgtcgatg tgttcacgca ggtcgaggag gaatactttg ccatccgcaa
    14401 tgcggcgagc gtttatgacc tgacaccgat gatcaagtac cggatcacgg gacctggggc
    14461 gctggcctac ctgaatcgcc tcgtgacgcg cgatctgcgt aagctcaagg tcgcccgtgt
    14521 tgcttactgc gtctggtgcg atgatgcggg aaacgtgatc gatgacggga cggtgtttcg
    14581 gctcggtgag aacgagtatc gcctctgcac agcctaccgg cagctcgact ggctgctggc
    14641 cagcgcgatc ggcttcgagg gcgctgcggc tgtgcggatc gaggaagtga cggacgagat
    14701 cgccgcgctc tcgctgcagg gaccgacatc ctttgccgtg ttgaagacgc tcggcctgcg
    14761 gggcgtcgag cgactcaggc ccttcgagat cggtttctgc gcgctcgatg acatcccgct
    14821 caccatctcc cgtaccggtt tcacgggcga tctgggttac gagctgtgga tgagcccggc
    14881 gcacgcggta gcggtctggg atgcactcat ggccgcgggg cgctcgcgcg gcatccgtcc
    14941 gataggctcg cgggctctca acatcgcgcg gatcgaagcc ggcttcctgc agccgaacct
    15001 ggatttcatc tccagcgctc atacgcagcg ccccggtcgc gaccgcacgc cgctcgagct
    15061 cggtctggag tggctggtgg acttcgacaa agggcatttc acgggaagac gggccctgtt
    15121 ggcgcagcgc cgccagggcc tcaagcggcg tctggcggga ctcgacatcg aggggaacaa
    15181 gcctgcgcac aacgccctgc tgtatgccga gcgctcgggt cagcgccaga tcggcagtgt
    15241 cagctcggcg agctggtcgc cgacctgcaa acgtaacatc gcgctggcta tgctcgatgc
    15301 tcctcatttc gagagcgggc gcggcctgtg ggcggaaatc tatctcaatc aggagctggt
    15361 atgggagcga cggatggttc gcgcacgggt cgtcgatcgc ccgttcttca accctgaaag
    15421 gcgcaagctc acccccccaa gagacttctg acccaccgca cagcaccgcc cgctgcacgc
    15481 taatgatcac gagccagcag aatcccgtgg ccgagcggcc gtggctggac ccgaccgcca
    15541 cgccgcagat cgtcatccgg ggcgtcagca aaatctacgg cgccttcacg gcggtggacg
    15601 acatcaacct gacgatcttc aagggcgaga tgttcgccct ggtgggagcc tcgggttgcg
    15661 gcaagacgac gctgttgcgg atgctcgccg gatttgcgga ccagagtgcc ggccagatca
    15721 cgatcgacgg cgtcgagatg gcgggcgtgc cacctcacga gcggcccgtc aatatgatgt
    15781 tccagtcgta tgcgctcttt ccgcacatga gcgtcgagcg caatgtcggc tacggcttgc
    15841 ggcgcctgga tctcgctcgc gcagagaagg agcgccgcgt ccaggaggcg ctcgagatgg
    15901 tgcaactcgg gcatctgcgt gcgcgcaagc cgcaccagct ctctggtggt cagcgccagc
    15961 gtgtggccct cgctcgggct ttgatccgcc gccccaaagt gctgctgctg gatgagccgc
    16021 tctccgctct ggacaagaag ctgcgcgaga agacccaatt cgagctcatg gatctgcagc
    16081 atcaggtggg catcaccttt atcgtcgtga cccacgatca ggacgaggcg atggccctcg
    16141 ccacccgcat cgccgtcatg gatcgcggcc gcatcgtaca ggtcggcaca ccgtcggaaa
    16201 tctacgagtt tccccagagc cgcttcgtag cggacttcgt gggtaccacc aacctgttcg
    16261 agggtacggt ggcggcgtgc gagccaggac tcatcaaggt gcgctgcaag gagactggct
    16321 gcgatctgct ggtcgatgat gttggaacct tcgcgcccgg gcagcaggtc tgggtggcgc
    16381 tgaggccgga gaagatccgc ctgagcaagc agcctgcatc accgggcacg atcaatcagc
    16441 tgcacggcac ggtctgggag ctcggctatc tgggtaaccg ctccacctat cagatcagga
    16501 cgagcagcgg caagctgatc accgtatttg cacagaacga gcggcgcacc tcgcaatggt
    16561 cgatcgactg gagcgatgag gtctacgtga cctggcaggc ggatgctgcg gtcatcctgc
    16621 aggcctgagc cccgaggaac ttcagagcat cttgtagccg aacgtcagtc cgatcacacg
    16681 cggacgcagg ggagtctgcg cgatgatgaa ctggtcggta ctgacgaacg tgctcgcgtt
    16741 ggagttgctc agattctcga tgtagagatc ggcataccag gcatccttgg caagaccgac
    16801 cgaggcatcg taggtggtgt aagcgggatt ctcgaagcgc aacctgccgg tgctgaccgc
    16861 gcccgcttcg gcgaaggtcg ggttcgaccc ggcctgggta aaggaatgcc cgctgtgggt
    16921 ggcccccagc tgcacatagg gcgtatagct tccgatcacc cactcgtacc gcgcccgcag
    16981 ggcgaactgc agcggggggg cgtccgcgct cggtgagccg accggcccga aggggttggt
    17041 gatcggggcg cagttggctc cggtgctgtc gcaggtctgc gagatcggct tgccgaagtt
    17101 ggcgctggcc ggattgttgt cgatgagcag cggcgagttc gtctgccggc tctgattcca
    17161 ggatgctgag ccctgcagcg tcagtccggt gacgagcctc gcgacgagct gtgtttcgac
    17221 ccccttgatg aggaagttct gcccgttggt gttgaagaaa atgttgccga cgacacccgg
    17281 atcgaagaac gcgacctgca cgtcatccca gttctcgcgg tagatggcac cgttccactg
    17341 cagacgatga tcgaggaatt cgctcttcca gccgatctcg ttgttcgtca gcttgtcgga
    17401 gtgatacgcg ctcggaatga tgaactgcgc ctctccatcc gtgccggggg cgtgcgccgc
    17461 cccgccgttc tgattgaagc cacccgggcg gaatccctgc gagtaggtgt agtagagcat
    17521 cagatcgggc gagagatgcc aggtgaggtt gccgcggctc ttgaagccgg actcggtgtc
    17581 gcgcaggttg gccccattca ggttgaaggc ctccgcctgg cagccgactg tgggggctcc
    17641 gccgtcaaag cagccgaaac tgcctgtcac gctgccgtcg gaggagttgt tgaagcggaa
    17701 gtggcgcgtc ccggcggtga cggtcaggac cttcggaatg atgtcgaagt ccaccgatgc
    17761 gaagaaggcc gtctgctttg tctggcgtac ggtgtcctgg taaaaggaag tcggcgccgg
    17821 gtggacgccg gggaagacga cggtcgctcc aggggcggtg ccgatgtctg cgaagcagcc
    17881 ggagttgccg ggtgagcccg ccggaccgtt cgcggtgcac gaggggacgc tcttgtagcc
    17941 ccagccggtc tcatcgaaca gtgtgttgtt ctcccagaac acaccggcga tcccgcgaaa
    18001 gcgccactcg tccggcgtgc tgaggcggaa ctcgtgctgc tgatgcgtat tgcgctcgct
    18061 cgagcgccac actgcgctcg gcgaaaagca ggtcggcttg agattcgcct ctccgcccga
    18121 gccggggcca aagcattggt agtagtcggc atacaccccg cgcgcatagt tggtgtagtc
    18181 acccacctgc tggacattgc ggaccaggta tccgccggta tagacggcct tgaggaaggc
    18241 gatcttgccg ttcagcgtcc acgcggtgct ttcgaacttg tccgtgtcga aggcgttgtt
    18301 gaaaagggtg acctcgagcg ggcgtagcgg cgcaccatcg gaggcgttcg gttgctgata
    18361 gaacaccccc tccgcatcca tctgctgata ggactgcgtg atgagggcat cccagtcatc
    18421 gttgaatttg tataaggcct cgacccgcgt ccccttgtag gtgaccggat tgatggcacg
    18481 acttgcgata ctggagttgt tgatggacgg gctgcccggc gggacgcaga agccattgtt
    18541 cggcaatcca tccggacact ggccgttgac ggccgggaaa ttcgcatagt gaatgccgat
    18601 gtcggtgttc ttgcgggtga atgtcgccgg cacgttgtcg atgtaaccgc cgcgccggtc
    18661 gtcgtagatg actgcacgca gggccatgtg atcggcgatc agcggcaggt tcaggaccgc
    18721 cgtgacatcg gtattggggt cgccatgagc ggtggcgcca tagcccgcct tgatgttggc
    18781 ctcggtcgta tcgagcttgg gctcgttggt gatgtaacgg atgacgcccg cctcggcccc
    18841 tgcgccgaac agcgttccct gcgggccttc caggacctcg atgcggttca gatcggcggc
    18901 gtacacatcc aggttgcgat tgggcagctg ccccgactgg ttgtccaggt agatggcgac
    18961 gttgggccag aggcccgttg aggcgctgcc ctggctcggc tgcgagccgg cgctcaggcc
    19021 gcgcatgaag atctcgttct gccccgggcc gttgttggca gtggtgacgt tcggcaggta
    19081 cttgatgtag tcgtcgaagg tcgccacgtt caactgctgc agcgtctcgg aggtcatcgc
    19141 ctggatcgag atcggcacat cctgcatgtt ctccgagcga cgctgggccg tgaccacgac
    19201 ctcctggatc gcatcgctgg gcgcagcact cgtgtcggcg ggttcgactg cataggcggc
    19261 accgatggag gagccgctga ggatcgcagc aatcgcggac gagaccttcg aattcatgtt
    19321 ggatcctttg ttttcttgag atttcaggcg cacgcatggg ccgtgcgcca tccggcgctt
    19381 ggaggatcgt ccctgaaggg ggcccgccag cgggcctcga gctactcgaa gacgcgggcc
    19441 cgggctgcgt ctccgtaagt ggtgcggttc ggctgcaggc ggcgcgtcga gggagaaagc
    19501 ccgtaggcgt ccttgaactg ccgcgagaaa tgcgcgcagt ccgcgaatcc cgcctcgatg
    19561 gcaatctccg tcaccgaccg atcggtgttc tccaccagcc agcgggcata ccgcatgcgc
    19621 agctggcggt acagagaggc ggggcccatg ccgacgtgct cccgacacag ccgctcgagc
    19681 tgtcgcaccg acacacccag ctcatcggcc acggttgcga tcgccaccgg gcgagttaga
    19741 ttctgctcca tcagcagcaa cgcgcggcgc acgcggggtt cgcggacgct ctcggaaagc
    19801 ggcgggtgcg gttgagtgtc gcttccggag cgcgcccggt cgaagagcaa cacctgactt
    19861 gccttctgtg caacggatcg cccgagatgc tgctcgatga ggaaggtagc gagcgcggcg
    19921 gtgccggcgc cgccggcaca ggtgatgcgc ggtccatcgg cgagaaacag acgatccgct
    19981 acgacctcct gaccggggaa ggcctccaga aaatcctggt agtgatacca gctcacgcag
    20041 gaccgtcgtc ccttcatcag tcccgcgcgg cacagaatga aactgcctgt gcagatgcca
    20101 atgagggggg tggtgctggc agcgacatcg cgcagatagc gcagcgtcgc ctcatcgacc
    20161 tgggggcccg cgtgcaaaat tcctccgacc accacgacgt agtccagcga ttcgggctcg
    20221 aggaagttgc tcgtcggctc gatcagcacg ccgcagctgg ccggaacgga gctctcgcgg
    20281 ctggccatga tggaccattg cacgtgcaag ggccggctgc ggtctccctc atcggctgcg
    20341 agccgcagat gatcgatgaa gactgcgaaa gcggacaggg tgaagtgatc ggcgagaatg
    20401 atcccgacgc gcaatggcac tcgcgtcacg ccggatggga gtacgggttt ctggaaaccg
    20461 cctggctgac ccattgaacg gccactcaat ttatcgatag acccctctcg cagctatatt
    20521 ccaccaagcc ccgagctgat gcaaggagca aagctgcgat cacaaatcct gagttatcac
    20581 aatcacttgg aggcgctcag ggcgctcttc gaggctcgag gaccgctcag tcttgattga
    20641 atgtacattc aggcagctca gaaaagagcc gggcgtctca gcgcaagact gcgagccgcg
    20701 ccaggttggc ctgggccacc gcatcgtggc aggcggagtg cgcataccag cggcgcacac
    20761 cttcgacgac cgcggtgcag atctggacgg catcggcggc ctccggatct tgcgtgtccg
    20821 gcatctcgat attgaaccca cggggggcgt ttcgagcgtg gcgcctcagt cgcagacgtc
    20881 tcagcgcggt tgacggaaca ccctcgttgg cggcctcgag atccgtcgct acatgctccg
    20941 aaagcacgcg gcagaggtct ccgtgatcca tgccggcggc gtgttcgtcc tcattggctg
    21001 gacgaatcca ctcttcgcac caggccttgc agcgctcgct ggacacgcac atctgcggat
    21061 cggtgagggc gaccgcgata cgaggcagat cgatcgcatc acgcagcgcc atctcccagt
    21121 gctgctcgct cagcagctgc tccacgtccg ccaggtacgt cgtgagcatt ctcgagctcg
    21181 aaggctccgg ccacggcttc ggatcaggcg tcaatgcagt gatgcgattc gaatcattcg
    21241 acatcgttgg gtcctcgcga tcaggtgtcc ggcgcagcca aacgcacttc aaattacaac
    21301 gggttcactc aacgctgggt ccggcgtctc ttgcgcctca cggcagccgt ggcgctgccg
    21361 ctggcacggc gcggtacacc gcggcttcgt ttcggccgcc cagtggacat cgtctccttg
    21421 tccggcacgg ctcgagccgc agtctgcgct atcacgatgg aatcattcct ctttgcgcgc
    21481 cggcgcgcac gcttccattc cttgcgggat gaggaggcga gcttgcgggc gttacgagcc
    21541 tcgcgcttgg cctccttgag acgcttgcgt gcgcgcttca gctcatcctt cgcaatccgc
    21601 acgcgctgct tggccacctc ggcctgcaag cgcactcgct caaggggatc caccgtgtgc
    21661 aactcccctc gcgctgcgga ggaggtctgg gatcgctgag cggcggacag ttcgctacct
    21721 gttcttttca tgcaccggtc tgcgaagaat gcggacggca agtctcacat atgcggacac
    21781 tgcttgccac cctggctccg ctcgcgctac ccctgctgaa tcgctgcatc agggcacagc
    21841 gcatttccca gttatccaca aaatttgtgc ataaccatgt ggaccagggg ctcggtgcgg
    21901 gctcaaagat cagctcacgc aagggcttgc atcaaagtga gcgatctttg gccaccttgc
    21961 cgtgcacgct catcgggcgc cccgtggccg tcgtgcggtg gagcgttcac ccttcgatgc
    22021 gggcagcctt gtagcagtca ctctattgcg gcctgcgttc ttgctgcgat acagcgcagc
    22081 atcggccacc ttgacgagac tctccgcaat gcgcttctca ccccccggca cgcgctcgat
    22141 gccgcagagg ccgagacttg cggtgacctc cacgcttcgg gccgaagtct cgaaagtctt
    22201 gttggcgatg gttgcacgca gcttgcgcgc gacatcgagt gcctgctcgt agccagtctc
    22261 ggggagtacg acggcgaatt cctcgccccc gattcgcgct acccaatcca cgccgcgacg
    22321 caacgcgtcc tgcaggcgcg cgccgaactg gcgcaggatc tcatcgccgg cggcatggcc
    22381 caaagtgtcg ttcacgtttt tgaaatggtc gatgtcgcac aggatcagcg acagtgcacg
    22441 gccgtagcgc gccgcgcgtt ccacttccct aggaaagtgc ttggtgaaaa agcgccggct
    22501 cgcgacgcgc gtaagatcgt ccgtggtgga gagcttgcgg ttctcgacga gagcgatccg
    22561 cagcacggcc tctagttctg cgatgcggcg cgcagccgat actcgcgcgt gcatctctcg
    22621 ctccggagcg cgtctcccga tgcagtcatc cgcgcccgcc aggaggccgg cgtcgcgctc
    22681 ggacgcttca tccagctccg ccacgtagag tatgtagggc gtccgctcgg acttgcgtgc
    22741 gcggatcttt cggatcagct cggggttgtc ggtgagaatg acggggtaga actcagcggc
    22801 gaagcggcga acggcctcct gctcctcggt aatcgcttcg ccatcgagca actcggaggc
    22861 gattcgtcgt tgcaaatggg agagaagcgc agaatcctcg aacaccagca gcgcccgggg
    22921 aggcggcaga acacgcagca tgacagcgat ggttgcggag gtatcctcct gaatcgcagg
    22981 cggagtctgc gcccgacgct cgagaatgcg ttgaatgacg gtagtctgac cgcc
//
LOCUS       DASRHN010000287         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2240873, whole genome shotgun sequence.
ACCESSION   DASRHN010000287 DASRHN010000000
VERSION     DASRHN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240873"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFB37_11580"
     CDS             <1..553
                     /locus_tag="VFB37_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO23132.1"
                     /translation="GKIVYVGSSNFAGWHLAAAQEAARHQQVFSLVSEQSIYNLLNRW
                     VELEVLPATRHYGIGLIPWSPLQGGLLGGVLRKQREGSASRSTSDFSAGRLETNRERI
                     DAYEKLCADIGEDPANVGLAWLLHQDGVTAPIVGPRTTAQLDSSLRALAITLDAQVLA
                     RLDELFPPPGPNGAKPAPEAYAW"
     gene            complement(746..934)
                     /locus_tag="VFB37_11585"
     CDS             complement(746..934)
                     /locus_tag="VFB37_11585"
                     /inference="COORDINATES: protein motif:HMM:NF039659.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HZO23133.1"
                     /translation="MYFTDRGIEELEDRRASEQISVEWLAARMRAFVDQHPEFEDAVE
                     ALATFLARDDADADDFAL"
     gene            complement(1089..>2381)
                     /locus_tag="VFB37_11590"
     CDS             complement(1089..>2381)
                     /locus_tag="VFB37_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZO23134.1"
                     /translation="AERALKDYAQQLEHVFNEVRELERGAVAPSESVEAEQLVPTGLD
                     TGVDISVIERIADAHVNLPEGFTSHPRVRPVLEKRAKMAREGGIDWAFAELLAFGSVC
                     LDGRMVRLSGQDSRRGTFVQRHSVLIDRKTGAEYTPLMHLSPDQAKFLAYDSALSEFA
                     ALGFEYGYSVANPDALVLWEAQFGDFANGAQPVIDEFISSGEAKWGQRSDVVLLLPHG
                     HEGQGPDHTSGRIERFLQLCAEGSMTVAQPSTPANYFHLLRRHALDGVHRPLVVFTPK
                     SMLRHKAAVSPVEDFTSGRFASVLDDPTITDPSAVRRVLLCSGKISYELHAGRQQRGI
                     HDIAIVRIEQLYPLPQRRLAAALDRYPEATDVCWVQEEPANQGAWPFYGLTLPELMPE
                     RLSRLRRISRRAMAAPSAGSAKVHEVEQRELIAAALDA"
BASE COUNT          399 a          776 c          793 g          413 t
ORIGIN      
        1 gggtaagatc gtttatgtcg gctcatcgaa tttcgccggc tggcacctgg ctgctgccca
       61 ggaggcggcc cgccatcagc aggtgttcag cctggtcagc gagcagtcga tttataacct
      121 gctcaaccgg tgggtcgagt tggaagtgct gcctgccacc cggcactatg gcatcggtct
      181 gatcccgtgg tcgccgctgc agggaggctt gctcggcggg gtgttgcgta agcaacgcga
      241 gggtagcgct tcgcgaagta cctcggattt ctccgcgggc aggctggaga cgaaccggga
      301 gcgcatcgac gcctacgaaa aactctgcgc tgatatcggc gaggatccgg cgaacgtggg
      361 actggcctgg ttgctgcatc aagacggcgt gaccgcaccg atcgtgggcc cgcggaccac
      421 cgcgcagctg gacagttcgc tgcgggctct tgccatcacc cttgatgcac aggtgctcgc
      481 ccggctcgat gagctgttcc ccccacccgg gcccaatggc gccaagcctg cccccgaagc
      541 ctatgcgtgg tgacctccgc tgtcctcccc gcgcgtgtcg cgccctgccc cgcgcgtgtc
      601 gcgcgctggc cccgcgcgtg tcgcgcgctg accccaggcg tgtcgcgcgc tggtgccggg
      661 cgtgtcgcgc ccagaggtac agcggcaagg agcctgcccg atcgctgagt cagtttggca
      721 gcggcggcag ccgcgggact aacgttcaga gagcgaaatc atcggcgtcg gcatcgtcac
      781 gggccaagaa cgtggccagc gcctcaaccg cgtcttcgaa ctccgggtgc tggtcgacga
      841 acgcgcgcat ccgggccgcc agccattcga cgctgatctg ctcgctggcg cgccggtctt
      901 cgagttcctc gattccgcga tcggtgaagt acacctcgac tcctcctttt cgaggggcct
      961 gcgctgatac cggctggcgg agcgtacggg acccgtgcgc cagcgctcga cacaccgcgc
     1021 aggagagcac gacatgcgcg gcgggagagc gcgacacgcc ggaaaggggt ggggtggcgt
     1081 ggcggcggtt aggcatcgag ggccgcggcg atgagctcgc gctgctcgac ctcgtgcacc
     1141 tttgctgatc ctgcggacgg tgcggccatg gcccgccgag agatccgccg caacctgctc
     1201 agccgctcgg gcatcagctc gggaagggtc agcccgtaga agggccacgc gccctggttg
     1261 gcgggttcct cctgcaccca gcacacgtcg gtggcctcgg ggtagcgatc cagcgccgcg
     1321 gccagccgcc gctggggcag tggatacagc tgctcgatgc gcacgatcgc gatgtcatgg
     1381 atacctcgct gctggcggcc ggcgtgcaac tcgtagctga tcttgccgct gcacagcagc
     1441 acccggcgca cagccgaggg atcggtgatc gtcgggtcgt cgagcaccga ggcgaagcgt
     1501 cctgaggtga aatcctcgac cgggctgacc gccgccttgt gccgcagcat tgacttgggg
     1561 gtgaacacca ccagcggccg gtgcaccccg tccagggcgt gccggcgcag caggtggaag
     1621 tagttcgccg gcgtggacgg ttgcgccaca gtcatggaac cttccgcgca tagttgcagg
     1681 aaacgctcga tgcggccgga agtgtggtcg ggaccctggc cttcgtgacc gtgcggcaac
     1741 aacaagacga cgtcggaacg ttgcccccat tttgcttcac cggaggaaat gaactcatcg
     1801 atcaccggct gcgcgccatt ggcaaagtcg ccgaactgtg cctcccacag taccaaggca
     1861 tcggggttcg ccacggaata accgtactca aagccaagtg ccgcgaattc tgacaatgcc
     1921 gaatcgtagg ccaagaactt cgcctggtcc ggcgagaggt gcatcagcgg ggtgtattcg
     1981 gcaccggttt tgcgatcgat gagcaccgag tggcgttgca caaaggtgcc gcgccggctg
     2041 tcctggccgg ataaccgcac catgcggccg tccaggcaaa ccgacccgaa tgccagcagc
     2101 tctgcgaatg cccagtcgat gccgccttcg cgtgccatct tcgcgcgctt ttccagcacc
     2161 ggtctgaccc ggggatgaga tgtgaaccct tctgggagat tgacgtgagc atcggcgatc
     2221 cgttcgatga cgctgatgtc aacgccggta tccagcccag tcggcacgag ctgctcggct
     2281 tccacagact cgctgggtgc cacggcgccg cgctcaagtt cgcgcacctc attgaagaca
     2341 tgctcaagct gctgagcgta atctttcaga gcgcgttcgg c
//
LOCUS       DASRHN010000288         5825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2240900, whole genome shotgun sequence.
ACCESSION   DASRHN010000288 DASRHN010000000
VERSION     DASRHN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240900"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFB37_11595"
     CDS             <1..777
                     /locus_tag="VFB37_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO23135.1"
                     /translation="AVKGVLSGRHAVITGAGRGIGAAIAARLAAAGARISLLGRELTS
                     LQSSARQCGGEGGSRAIVADVTDAGSVREAFSAARTGFGPVHILVNNAGQAASAKFTD
                     TDENLWSRLIAVNLTGTFLCTRQAVPDMLQAGFGRIVNVASTAGLRGAPYISAYASSK
                     HGVVGLTRSLALEYATRHITVNAVCPGFVDTGIARKAIADISAKTGRSESESRAALVA
                     ANPQRRLIEPREVADTVLWLCGPDSESVSGQTIVLGGEAS"
     gene            790..1302
                     /locus_tag="VFB37_11600"
     CDS             790..1302
                     /locus_tag="VFB37_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO23136.1"
                     /translation="MPKDKTTLKLVEPRVDAETRIHDDHHVSVRLWLRMLACTNRIES
                     FVRQNLQASFATTLPRFDLMAQLERAPQGLKMSELSQRMMVTGGNVTGITDGLEKEGL
                     VVRESDPADRRVFRVKLTAEGQRQFRRMAADHEQWVIALFDGLSMKHKNQLVELLGKL
                     KRHISRQPAG"
     gene            1317..2126
                     /locus_tag="VFB37_11605"
     CDS             1317..2126
                     /locus_tag="VFB37_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HZO23137.1"
                     /translation="MKPFEPQPQHFLWQRDGRVGLITLNRPERKNPLTFESYAELRDL
                     FRALAATREIRALVLSGSGGNFCSGGDVHEIIGPLTQRDARGLLEFTQMTGDLVKAMR
                     HCPQPIVAAVDGACAGAGAILAMASDFRLGTARAKTAFLFTRVGLAGCDMGACALLPR
                     IIGQGRAAELLYTGRAMTAEEAAAWGFFNRLVAPEQLLAEALQLAREIAAGPTLAHAT
                     TKRMLHEEWHMPLDAAIDAEAQAQAECMQTEDFRRAYRAFAAKQKPRFEGD"
     gene            2131..3336
                     /locus_tag="VFB37_11610"
     CDS             2131..3336
                     /locus_tag="VFB37_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO23138.1"
                     /translation="MPARSFLDWPFFSGEHRELVARLDHWAAERFRSPDRSGTEDRAG
                     VDASCREWVRELGRAGWTKYSAPGASEPPAAAFDVRALALIREILAWHDGLADFAFAM
                     QGLGSGAISLAGSAELRNRYLPRVVRGEAIAAFALSERDAGSDVAAIECRAHRDGEAY
                     VLNGEKTWISNGGIADFYCVFARTSPAEHRPDGSVTAKGISAFVVDADTAGFSAAARI
                     ELIAPHPLATLSFRDCRVPVSHRLGEEGAGFKLAMRTLDVFRTSVAGAALGFAQRALD
                     ESLRHALGRRMFGRTLAEMQLTQAALAEMATEIDAARLLTYRAAWLRDRGATVTQEVA
                     MAKMAATESAQRAIDRAVQMHGGRGVTQGEIVEHLYRDIRALRIYEGATEVQKLIIAR
                     ELLNTARPH"
     gene            3348..3761
                     /locus_tag="VFB37_11615"
     CDS             3348..3761
                     /locus_tag="VFB37_11615"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZO23139.1"
                     /translation="MQILKPPQWPRPSGYSHGMAASGRLIFVAGQVGWDAQCRFQSTQ
                     LADQVRQALENICAVLAEVHAGPEHIVRLTWYVTSRDEYHAELDDIGAAYRAVMGKCF
                     PTMSVVQVLALMEPDAKVEIEATAVLPEATSSETR"
     gene            3768..5234
                     /locus_tag="VFB37_11620"
     CDS             3768..5234
                     /locus_tag="VFB37_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23140.1"
                     /translation="MWRPPERIRHDSRPGRWPTAAEAATSRLFSPVRVGPLTLAHRTW
                     VPAMVPWRASEDGLVTAEVLDWYRCFATGRPAGLVVEATGIRDVPSGPLLRAGHDRFL
                     PGLRELVRVVREASDGQTRLFVQLIDFLAIRRRPQPSRYFAQFLSIAPRHRLAFGNRG
                     ASDDEVRAWLAALSPEELGRVLDARELESLQFGARERVTDTHLAHVRDLPLRLPGLFA
                     AAARRARDAGFDGIELHFAHAYTMASFLSRSNCRVDGYGGEIANRARLALEVFARVRS
                     EVGGDFPVGCRFLADECIAGGSDVGDAEYFAGEFARAGMDFLSLSRGGKFDDAKQPKI
                     GEAAYPYTGRSGYECMPSYFSDARGPFGRNLESVARVRQAVRARGLQTPVVATGGIHS
                     FEQAESVLASGQADIVGLARQALADPDWFRKVASGHGAQVRLCLYTNYCEALDQRHRQ
                     VTCELWDREALEEPGVKRSADGKRRLTPPSWNPPDDGV"
     gene            complement(5303..>5825)
                     /locus_tag="VFB37_11625"
     CDS             complement(5303..>5825)
                     /locus_tag="VFB37_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HZO23141.1"
                     /translation="TVGLSLVARGEGSSYAADRRHCLAQDDRLPGHHAHGIGEIMSSS
                     ISRRRLLSLGMASGTAAIIAGLRPQVALTATGQQAASTGASPGRQNSSSTSAPGPKVS
                     EKDSLAVALGYVADASRIDRSANPTYQAGASCSTCSWYQGKPGDAHGGACTFFPGKLV
                     EPHGWCKMWNRKQ"
BASE COUNT          953 a         1898 c         1965 g         1009 t
ORIGIN      
        1 gctgtcaagg gtgtgctgtc gggccggcat gcggtcatca ccggcgccgg ccgcggaatc
       61 ggcgcggcca tcgctgcaag acttgccgca gcaggcgccc gcatctcatt gctcggccgc
      121 gagctgacat cactgcagag cagtgcgcgc cagtgcggtg gtgagggtgg cagcagagcg
      181 atcgtcgcgg acgtgaccga tgccggttcg gtgcgcgagg cattctcggc ggcccgcaca
      241 ggtttcgggc ccgttcacat tctggtcaac aatgccggac aggccgcgag cgccaagttc
      301 accgacaccg acgagaatct gtggtcgcgg ctcatcgccg tcaacctcac cggcaccttc
      361 ctgtgcacgc gtcaggccgt acctgacatg ttgcaggctg gcttcggacg gatcgtcaat
      421 gtcgcgagca ccgctggcct gcgcggtgcg ccctacatat ccgcgtacgc cagctccaag
      481 catggggtcg tcgggctgac gcgctcgcta gcgctcgaat atgcgacccg ccacatcact
      541 gtcaacgccg tctgtcccgg cttcgtcgat accggcatcg cgcgcaaggc gatcgccgac
      601 atttccgcca agaccggtcg aagcgaaagt gagtcgcggg cggcactggt tgctgcgaat
      661 ccacagcggc ggctgatcga gccgcgtgag gtggccgaca ccgtgctgtg gctctgtggg
      721 ccggactccg agagcgtatc gggacagact atcgtgctcg gcggcgaggc gagctgaagg
      781 ggaagccaga tgccgaagga caagacgaca ctgaagctgg tcgagccgcg cgtggacgcc
      841 gagacccgca tccatgacga tcaccacgta tcggtccggc tttggctgcg catgctcgcc
      901 tgcaccaacc gtatcgaaag cttcgtgcgg cagaacctgc aggcgagctt tgccacgacg
      961 ctgccgcgct tcgatctcat ggctcagctc gaacgcgcgc ctcagggatt gaagatgagt
     1021 gagctctcgc agcggatgat ggtgacaggc ggcaacgtca ccggaatcac cgacggcctg
     1081 gagaaagagg ggctggtcgt acgcgagagc gatccagcgg accggcgagt cttccgcgtc
     1141 aagctcacag cggagggtca gcggcagttc cggcgcatgg ccgccgatca tgagcagtgg
     1201 gtgatcgctc tgtttgacgg actgagcatg aaacacaaga atcagctggt ggagttgctc
     1261 ggcaagctca agcgacacat cagtcgccag ccggccggtt gagtggaaac tcagccatga
     1321 agcccttcga gccgcaaccc caacacttcc tgtggcaacg cgatgggcgc gtcggcctca
     1381 tcacgctgaa ccgcccagag cggaaaaacc cgctgacctt cgagtcctat gccgagctgc
     1441 gggacctgtt ccgggcattg gccgcgacgc gcgagattcg agccctcgtg ctgagcggct
     1501 ccggaggaaa cttctgctcc ggaggcgacg tgcacgagat catcggcccg ctcacgcaga
     1561 gagatgcgcg cgggctgctc gagttcacgc agatgacggg ggatctggtc aaggcgatgc
     1621 gccattgtcc gcagccgatc gtcgccgcgg tggacggcgc ttgcgccggt gccggcgcca
     1681 ttctcgcgat ggcttccgat ttccggctgg gcacggcgcg ggcaaagacg gccttcctgt
     1741 tcacgcgggt ggggctggcc ggttgcgata tgggtgcctg cgcgctgctg ccgcggatca
     1801 tcggtcaggg acgcgccgcg gagctgctgt acaccggccg cgccatgacc gccgaggagg
     1861 cagcggcctg gggcttcttc aaccgtctgg tagcccccga gcagctgctg gccgaagcgc
     1921 tgcaactcgc cagggaaatc gccgcgggac ccaccctcgc ccatgcgaca accaaacgca
     1981 tgctgcacga ggagtggcat atgcccctcg atgcggcaat cgatgcggag gcccaggcgc
     2041 aggccgaatg catgcagacc gaggacttcc gtcgcgccta tcgggcattc gcggccaagc
     2101 agaagccccg attcgaggga gactgagtcc atgcccgctc gcagcttcct cgactggcct
     2161 tttttctccg gcgagcatcg cgagctcgtg gcccgtctcg atcactgggc cgcagagcgc
     2221 ttccgctcgc ctgatcgctc cgggacggag gaccgcgcgg gcgtcgatgc atcctgccgc
     2281 gagtgggttc gcgagctcgg ccgcgccggc tggacgaagt actccgcacc cggcgcgagc
     2341 gagccgccgg cagcggcgtt cgatgtgcgg gcgcttgcgc tcatacgcga gatacttgcg
     2401 tggcatgacg ggcttgcgga cttcgccttt gcgatgcagg ggctggggag cggtgcaatc
     2461 tcgctggccg gctcagctga gctcagaaat cgctatctgc cacgcgttgt gcgcggcgaa
     2521 gccatcgctg cctttgctct ttccgagcgc gacgcgggct cggatgtcgc cgccatcgag
     2581 tgtcgcgctc atcgcgatgg cgaggcttat gtgttgaacg gcgagaagac gtggatctcc
     2641 aacggtggga ttgcggactt ctattgcgta ttcgcgcgta ccagccctgc cgaacatcgg
     2701 cccgacggct cggtcactgc caaaggtatc agtgccttcg tggtcgatgc ggacaccgcg
     2761 ggcttctcgg cggcggcccg catcgagctc atcgcgccgc atccactcgc cacactctcg
     2821 tttcgcgatt gccgcgtgcc ggtgagccat cgactgggcg aggaaggcgc ggggttcaaa
     2881 ctggccatgc gcacgctgga tgtcttccga acctcggtcg ccggcgcggc gctgggtttt
     2941 gcgcagcgcg cactcgatga gtctttgcgc cacgcactcg ggcggcgaat gttcggcagg
     3001 acactggccg agatgcaatt gactcaggcg gccctcgctg agatggctac cgagatcgat
     3061 gccgcgcggc tgctgaccta ccgggccgcc tggctgcgcg accgcggcgc caccgtgacg
     3121 caggaagtcg ctatggccaa gatggctgcc accgaatccg cccagcgggc aatcgatcgt
     3181 gccgtgcaga tgcatggggg tcggggcgtg acgcagggag agatcgtcga gcatctgtat
     3241 cgcgacattc gcgccctgcg catctatgaa ggtgcgacgg aggtgcagaa actgatcatt
     3301 gcccgcgagc ttctcaacac agcgcggccg cactgagcga ggcctccatg cagattctca
     3361 aacctcccca gtggccgcgt ccgagcggtt acagccacgg gatggccgcc tccggccgcc
     3421 tgatcttcgt tgcgggccag gtgggctggg acgctcagtg ccgcttccag agcacgcagc
     3481 tggcggatca ggtcaggcag gccctggaga atatctgcgc ggtactggca gaggtgcatg
     3541 ccggccccga gcacatcgtg cggctcacct ggtatgtcac gagccgcgac gaatatcatg
     3601 ccgagctcga cgatatcgga gcggcgtacc gcgcagtcat gggcaagtgc ttcccaacca
     3661 tgagtgtggt gcaggtgttg gctctcatgg agcccgatgc caaggtcgaa atcgaggcga
     3721 ccgccgtgct gccggaggcc acctcgtccg agacacggta gacatccgtg tggagacccc
     3781 ctgaacggat ccgacatgat tcccgccccg gcaggtggcc gactgcagcg gaagccgcta
     3841 cctcccggct gttctcgccc gtacgggtcg gacccctcac tctcgctcac cggacctggg
     3901 ttcctgcgat ggtgccgtgg cgcgccagcg aggacggtct cgtcacggcc gaggtcctcg
     3961 actggtatcg gtgctttgcc acagggcgcc cggccggcct cgtcgtggaa gcgaccggaa
     4021 tccgcgacgt gcccagcggg cctctgctgc gcgcgggcca tgatcgcttt ctccccggcc
     4081 tgcgtgagct cgttcgcgtc gtgcgtgagg ccagcgacgg gcagacgaga ctgttcgttc
     4141 agctcatcga cttcctcgcg atccggcgcc ggccgcaacc gtcccggtac ttcgcgcaat
     4201 ttctgagcat cgcgccgcga catcgcctcg cctttggcaa ccgcggcgca tcggatgacg
     4261 aggtccgggc ctggctcgcc gctctgtcgc ccgaagagct gggccgagtg ctcgatgccc
     4321 gggagctgga gtcgctgcaa ttcggtgccc gcgagcgggt cacggacacc catctggcgc
     4381 atgtgcgcga tctgccgctg cggctgccgg gattgttcgc agccgccgca cggcgcgcgc
     4441 gggatgccgg attcgatggc atcgagctgc acttcgcaca tgcctacacc atggcctcgt
     4501 tcctgtcgcg atccaactgc cgcgtggatg gctacggagg ggaaatcgcc aaccgcgcgc
     4561 gattggcgct cgaggtattc gcacgcgtac gcagcgaggt tggcggggac ttccctgtcg
     4621 gatgccgatt cctcgccgac gagtgtatcg ccggcggctc cgacgtgggc gatgctgagt
     4681 atttcgctgg cgagttcgcg cgagcgggta tggacttcct gtcgctctca cgcggcggaa
     4741 agtttgatga tgcgaaacag ccgaagatcg gcgaggccgc ctatccttac acgggccgta
     4801 gcggatacga gtgcatgccg tcgtatttct cggatgcgcg cgggcccttc gggcgcaacc
     4861 tcgagtcggt cgcgcgcgtg cgccaggccg tgcgtgcgcg cggtctgcag acccccgttg
     4921 ttgctaccgg cggcatccac agcttcgagc aggccgagtc ggttctggct agcgggcagg
     4981 cggatatcgt ggggcttgca cgccaggcgc tggccgaccc agactggttt cgcaaggtgg
     5041 cctcaggaca cggcgcccag gtgcgattgt gtttgtacac caactactgc gaggcgctcg
     5101 atcagcgcca ccgtcaggtc acctgtgagc tgtgggatcg cgaggcgctc gaagagccgg
     5161 gcgtgaagcg cagcgccgac ggcaagcggc ggttgactcc gccttcatgg aatccgcccg
     5221 acgatggggt ctgatcggcg cggcgcagag tcggtgaaca gatccgcgcg cgctgcgtta
     5281 cgcagcccgc cggttcaccc gcctactgct ttcggttcca catcttgcac cagccgtgag
     5341 gttcgacaag cttgcccgga aagaacgtgc aagccccgcc atgggcatct cccggctttc
     5401 cctgatacca cgagcaagtc gagcagctcg cgccagcctg ataggttggg ttggcgctgc
     5461 gatcgatacg gcttgcatcc gctacgtacc cgagcgcgac cgccagggaa tccttctcgc
     5521 tcactttcgg tccgggcgcg gaggtgctgc tcgagttctg ccgcccaggc gaggcgcccg
     5581 tgctcgcggc ctgctgcccg gtcgcggtca gggccacttg cgggcgcagc cctgcgatga
     5641 tcgcggcagt gcccgatgcc atacccaggg aaagcaagcg acgccgcgag atggaagaac
     5701 tcatgatttc accgatgccg tgcgcgtgat gacctggcag gcggtcatcc tgcgccaggc
     5761 agtgccgccg gtcagcagca tagcttgagc cctcccctcg cgctacgagg ctcaatccaa
     5821 ccgtg
//
LOCUS       DASRHN010000289         7988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_429774, whole genome shotgun sequence.
ACCESSION   DASRHN010000289 DASRHN010000000
VERSION     DASRHN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429774"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /locus_tag="VFB37_11630"
     CDS             <1..1742
                     /locus_tag="VFB37_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZO23142.1"
                     /translation="ALALSWLITRAMARPLSHAINVFGKISAGKYDNEIRLNGTDEAS
                     QVLSALREMQGKLRTQIETERAAAAENTRIRQALDKVSTSVVLADAQHKIIYLNDTAQ
                     ATFTRNQAQIRQSLPGFDAQHLQGSSVEALAADPARHRRMLDSLAGTDVEERKFGSCT
                     FRTVSNPVLSQTGERIGTVIEWTDRTPEVAVEKEMQSMLSAVIGGNLGRRIDLAGKSG
                     FFEVMSRGVNHLADNMAAVVAQVKSVAAEVHRGAEEISAGNANLSQRTEQQSSSLEET
                     ASSMEEMTTTVKQNADNAGQANQLAIAARDQAERGGSVVNQAVKAMADINDASKKIAD
                     IIGVIDDIAFQTNLLALNAAVEAARAGEQGRGFAVVASEVRSLAGRSATAAKEIKELI
                     QDSVRKVEDGSVLVTQSGQTLEKIVSAVKKVSDIVAEIAAASREQSSGIEQVNRAVVQ
                     MDELTQQNAALVEQATAASQAMAEQVRSLNQMLERFRIEGASGSGAVSSPLASPASTP
                     PTPRAQSTAAPERRSAKRPWSGKASARGGTSPTASERKKARGSSPPRTQPATDALASP
                     GTEGQEDSEWREF"
     gene            1773..2135
                     /locus_tag="VFB37_11635"
     CDS             1773..2135
                     /locus_tag="VFB37_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO23143.1"
                     /translation="MARILAVDDSVAMRQMVGLTLTGAGHQVEQASDGCEALQMASRT
                     KFDLVITDVNMPRMDGITLVRELRNLASYKYVPMLVLTTEATAQRKQEGKAAGATGWL
                     VKPFNPERLLATIARVLG"
     gene            2189..2521
                     /locus_tag="VFB37_11640"
     CDS             2189..2521
                     /locus_tag="VFB37_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23144.1"
                     /translation="MNSAKQILLALALGCAGCLPAYADCVLPPAPSKIPDGSTASQQE
                     MITAMQTLKEYNQDVETYTKCLDFEARQNRISSSDAQKMHNSAVDTLQKVAAKFNEQV
                     RTFKAKSG"
     gene            2521..2961
                     /locus_tag="VFB37_11645"
     CDS             2521..2961
                     /locus_tag="VFB37_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23145.1"
                     /translation="MKRIEEHARAPDSATGSAPGAADTSNCASAAELAGVLLAHTMQE
                     SEAPIAQLSEALARVARSLTGGSQPGPQAEVAALRLEAARGAIIRDLAVCIENLQFHD
                     RLMQQLTRVHDILAALAANRRLIDIPAQVPGQAAKGGHGSIELF"
     gene            2976..3842
                     /locus_tag="VFB37_11650"
     CDS             2976..3842
                     /locus_tag="VFB37_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HZO23146.1"
                     /translation="MNNTLLLDTQTMNRLREFAFSDEDFEALRKLVKQITGINLSEQK
                     RELVYGRLARRLRALQLRSFAQYREFLAGDSGSEIAQLCNAITTNLTSFFREPHHFDY
                     LREHVLEPLRASSARASRLRIWSAGCSTGEEAYSLGITVLETFPDASRWDVRILATDL
                     DSDVLAHARGGIYPAERLKNISAERRARFFVERSEADGPCYEVLPELKALIAFKQLNL
                     MHPLPMHGPLRAIFCRNVVIYFDKNTQRELFARLSRLQRPGDLLCLGHSESLFKVSDD
                     YTLMGKTVYRRV"
     gene            3842..4501
                     /gene="cheD"
                     /locus_tag="VFB37_11655"
     CDS             3842..4501
                     /gene="cheD"
                     /locus_tag="VFB37_11655"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF010013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HZO23147.1"
                     /translation="MRPDAPAPSKSRQPPMLPGFEHMQRFWDPQIGHWTVKILPGEYF
                     VTQGKDAISTVLGSCISACVRDPVRNVGGMNHFMLPEDASGGPNAWLDPAVGLATRYG
                     SYAMENLINDLLKLGAVRERLEIKVFGGGRVLSGLTDVGARNIAFIRNYVRLEGYRIA
                     AEDLGGTQPRKVVYFPESGLARMRRLRPVENRIISHHEQLYLASIGSKAAGGGDVELF
                     D"
     gene            4482..5654
                     /locus_tag="VFB37_11660"
     CDS             4482..5654
                     /locus_tag="VFB37_11660"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HZO23148.1"
                     /translation="MSSSSIDEIRDGIAMTHSAQSGPSPAKRPIRVLIVDDSALVRSL
                     LTSILRTDPGIEVVGVAGDAHVAREKIKLLQPDVLTLDVEMPRMDGITFLRNLMRLRP
                     MPVVMVSSLTERGADVTLDALALGAVDYLTKPRIDLAATLKDYSEELIEKIRTAARAS
                     VRALEPLRAIRPTANLTAILPQLPRARSLRTTDRFVAIGASTGGTEAIKEVLTRLPAD
                     SPGVVITQHIPKAFSTPFAKRMNDCCQLTVYEAEDGQQLLAGHAYIAPGDRHLMVVRD
                     GARYVLRLDDGLPVNRHKPSVDVLFRSVAQNAGPNAIGVLLTGMGKDGARGLREMLDS
                     GSRTIAQDEASSVVWGMPGEAVALGAAQHVLSLGTVAAKILALAESMDLGGQKREP"
     gene            5720..6604
                     /locus_tag="VFB37_11665"
     CDS             5720..6604
                     /locus_tag="VFB37_11665"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZO23149.1"
                     /translation="MNKASAQPDGAPGDPRAFAFVQQLASELSKGKVDLPSFPDVALR
                     VRKVLSDENVSQDKVVRVVGSEPALAARLMQIANSAALNFSGKPITELRTAIARMGFN
                     MVRSAAIAFAMSQLKKVDSLKGLEAPLEDLWKRSATVAAMSHAVARRFSRVNPDTALL
                     AGLLHGIGQLYILTRSSQHPGLFENQVAYAGIVRDWHSAIAKALLENWDMTEEIVEAV
                     SNYEDLERVHSGPVDLTDVLTVGNLLAAFKSHPENIELNMHDVAACKRMQADRAAYEE
                     LIKESEHEIDNLLQALGT"
     gene            6627..7523
                     /locus_tag="VFB37_11670"
     CDS             6627..7523
                     /locus_tag="VFB37_11670"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZO23150.1"
                     /translation="MSVAQSTSRTAEQQEATQGSVAFNFVQALAAELSSGKIELPGFP
                     HIVMRVQKILADENADTSRIVRVLGSEPVLASQIVCMANSAALNPGGAVVADLRAAVA
                     RVGLDTVRSATISFAVRQLHDAPLLKGLEAPLGVLWRRSVLVASLSCVIARRLTSVNP
                     DTALLAGLLQGIGRLYILTRASGHRTLFADQDIYRTIEQDWHLSIAAALLENWGIAEE
                     IVQAVQESENLEREARGLLSLTDVLVAATILAEHHNQPQALYAQMHCARPLQRLKLDA
                     SACEKFISESAQDIESLREALG"
     gene            complement(7581..>7988)
                     /locus_tag="VFB37_11675"
     CDS             complement(7581..>7988)
                     /locus_tag="VFB37_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HZO23151.1"
                     /translation="ALLEREHQALAAQDMEKIQSTARTRQEQLGSLAQIEEQRRALCR
                     LHGHSDDKPGLEALMSWCDPDGSLLPTLSDCAQRALRCRNLNDRNGALVTAMLRHVER
                     RLAVLRGESNMTVIYGPRGYSAAGQSKRILGAV"
BASE COUNT         1498 a         2601 c         2591 g         1298 t
ORIGIN      
        1 tcgcgctcgc gctttcctgg ctgatcactc gcgcgatggc gcgaccgctg tctcacgcga
       61 tcaacgtctt tggaaagatc tccgccggca agtacgacaa cgagattcgt ctgaacggga
      121 ccgatgaggc ctcgcaggtc ctgagcgcgc tgcgtgagat gcagggcaag ctgcgtaccc
      181 agatcgagac tgagcgcgcg gccgcggccg aaaacacacg catccgccag gcgctcgaca
      241 aggtctcgac cagcgtcgtg ctcgccgatg cccagcacaa aatcatctac ctgaacgaca
      301 ctgcccaggc gaccttcacc cgcaaccagg cgcagatacg gcaatccctc cccggcttcg
      361 atgcgcagca tctgcagggc tcgagcgtgg aggccctggc cgccgatccg gcccgccatc
      421 gacgcatgct cgacagcctt gcgggcactg acgtggaaga gcgcaagttc ggcagctgca
      481 cgttccggac ggtcagcaac ccggtgctga gccagacagg cgagcgcatc ggcacggtga
      541 tcgagtggac cgaccgcacg cccgaggtcg ccgtcgagaa ggagatgcag agcatgctga
      601 gcgccgtgat cggcggcaat ctcggccggc gcatcgatct cgccggcaag tccggcttct
      661 tcgaggtgat gagccgcggg gtgaatcatc tggccgacaa catggccgca gtcgttgcac
      721 aggtgaagtc ggtcgctgcc gaggtgcatc gcggcgccga agagatttcc gccggcaatg
      781 cgaatctgtc gcaacgcacc gagcagcagt cctcctcgct cgaggagacg gcctcttcga
      841 tggaagagat gaccacgacg gtcaagcaga acgcggacaa tgcaggccag gccaatcaac
      901 tcgccatcgc ggcgcgcgat caggccgaga gaggcggatc ggtcgtcaat caggccgtca
      961 aggccatggc ggacatcaac gatgcctcga agaagattgc cgacatcatc ggcgtgatcg
     1021 acgacatcgc cttccagacc aacctgctcg ctctgaatgc cgccgttgag gcagcgcgtg
     1081 ccggcgagca gggccgtggc ttcgccgtgg tcgccagcga ggtgcgcagc ctggccggac
     1141 gctcggcgac ggcggccaag gagatcaagg aactgatcca ggactcggtg cgcaaggtcg
     1201 aagacggctc agtgctcgtc acgcaatccg ggcagacact ggaaaagatc gtctcggcgg
     1261 tgaagaaagt gtccgacatc gtggccgaga tcgccgccgc atcgcgcgag cagtcttccg
     1321 gcatcgagca ggtgaaccgg gcggtggtgc agatggacga gctcacccag cagaacgctg
     1381 cgctggtcga gcaggccacc gccgcatcgc aggcgatggc cgagcaggtg cgcagcctga
     1441 atcagatgct cgagcggttc cggatcgagg gggcctccgg ctcgggcgcg gtatccagtc
     1501 cgctcgccag ccctgcaagt acccccccca cgccacgagc gcagagcact gcggctcccg
     1561 agcgccgtag tgccaagcgg ccctggagcg gtaaggccag tgcgcgtggc ggaacgtctc
     1621 ctaccgcatc ggagaggaag aaagcgcgcg gctcctcccc gccacgtaca cagcctgcga
     1681 cagatgcgct cgcctctcct gggaccgaag gccaggaaga cagcgaatgg cgcgaattct
     1741 gatcgccggc acgcttttca agaggtagcg ctatggcccg gatattggcg gtagatgact
     1801 cggtggcaat gcgccagatg gtgggtctga ccctcaccgg tgcaggccat caggtcgagc
     1861 aggcctccga cggttgcgag gcactgcaga tggcgagccg cacgaagttc gatctcgtca
     1921 tcacggatgt gaacatgccg cggatggacg gcatcaccct ggtgcgcgag ctgcgaaacc
     1981 tggcttccta caagtatgtg ccgatgctgg tcctgaccac ggaagcgact gcgcaacgca
     2041 agcaggaagg caaggcggcg ggcgccaccg gctggctcgt caagccgttc aatcccgaga
     2101 ggctgctggc caccatcgcc agagtgctcg gctgagcaca gcggtttcga gacatcagac
     2161 agaacaattg aatgcgagga cgatgatgat gaactcagcg aagcagatcc tgctggcact
     2221 cgcgctcggc tgcgcgggct gcctcccggc gtatgcggac tgcgtgctgc cgccggcacc
     2281 cagcaagata cccgacggca gcacggcctc gcagcaggag atgatcacgg cgatgcagac
     2341 cctgaaggaa tacaaccagg acgtcgagac ttacacgaag tgcctggact tcgaggcccg
     2401 ccagaatcgc atcagctcga gcgatgcgca gaaaatgcac aactcggccg tggacacgct
     2461 ccagaaggtc gctgcgaagt tcaacgagca ggtgcgaacc ttcaaggcga agagcggcta
     2521 atgaagcgaa tcgaagagca cgcgcgcgcg ccggacagtg ccacgggctc agcgcccggt
     2581 gccgcggaca cctccaattg cgcctcggcc gcagagctgg ccggcgtgct gctggcgcac
     2641 acgatgcagg aatcggaagc gccgatcgca cagctcagcg aggctctggc gcgcgtggcc
     2701 cgcagcctga cgggcggctc gcagccgggc ccgcaggccg aggtggctgc gctgcgtctg
     2761 gaggctgctc gtggagcgat cattcgcgat ctcgcggtgt gtatcgagaa cctgcagttc
     2821 catgaccggt tgatgcagca gctcacacgt gtgcacgaca ttctcgccgc gctcgccgcc
     2881 aatcgccggc tcatcgacat cccggcgcag gtgccgggcc aggccgccaa gggcggccac
     2941 ggtagcatcg agttgttctg aaaggacgcg tgtcgatgaa caacaccctg ctgcttgaca
     3001 cgcagaccat gaatcggctg cgcgaattcg cattcagcga tgaggacttc gaggcgctgc
     3061 gcaagctggt gaagcagatc accggcatca acctttcgga gcagaagcgc gagctcgtgt
     3121 acggtcggct ggcgcgccgc ctgcgggctc tgcagctcag gagcttcgcg cagtaccgcg
     3181 agtttctcgc cggtgacagc ggcagcgaga tcgctcagct ctgcaatgcg atcacgacca
     3241 atctcacctc gttctttcgt gagccccatc actttgacta cctgcgcgag cacgtgctcg
     3301 agccgctgcg ggcgagctca gcgcgcgcca gccggctgcg catctggtcc gccggctgct
     3361 cgacgggcga agaggcttac tcactcggca tcacggtact ggaaaccttc cccgatgcga
     3421 gccgctggga tgtacgcatt ctcgccaccg atctggattc ggacgttctc gcgcacgcgc
     3481 gcggtggcat ctatcccgca gagcgcctga agaacatctc cgccgaacgg cgcgcccgtt
     3541 tcttcgtgga gcgttcggag gcggacggcc cttgttatga agtgctcccc gagctcaagg
     3601 cactcatcgc cttcaagcag ctcaatctca tgcatccgct gccgatgcac ggtccgctgc
     3661 gggcgatctt ctgccgcaat gtcgtgatct atttcgacaa gaacactcag cgcgagctgt
     3721 tcgcgcgcct ctcgcgcctg cagcgccccg gtgatctgct gtgtctggga cactccgaga
     3781 gcctgttcaa ggtgagtgac gattacacgc tgatgggcaa gacggtgtac cggagagttt
     3841 gatgcgaccc gacgcgcccg caccctcgaa gtcccggcag cctccgatgc tgcccggctt
     3901 cgagcacatg caacgcttct gggatccgca aatcggccac tggaccgtga agatcctgcc
     3961 gggcgaatac ttcgtcaccc agggcaagga tgccatcagc acggtgctcg ggtcctgcat
     4021 ctccgcctgc gtccgcgacc cggtgcgcaa tgtcggcgga atgaaccatt tcatgctgcc
     4081 ggaagacgcc tccggcggcc cgaacgcgtg gctcgatccc gccgtgggac tcgccacccg
     4141 ctatggctcc tatgcgatgg aaaatctgat caatgacctt ttgaagctcg gggccgttcg
     4201 cgagcgtctg gaaatcaagg tcttcggcgg cgggcgcgtg ctgtccggcc tgaccgacgt
     4261 cggtgcccgc aacatcgcct tcatccgcaa ctatgtgcgg ctcgagggct accgcatcgc
     4321 tgcggaggat ctcggcggca cgcagccgcg caaagtcgtg tatttccccg aaagcgggct
     4381 ggcgaggatg cggcggctgc gtccggtcga gaaccgcatc atcagccacc acgagcagct
     4441 ctacctcgcc agcatcggca gcaaggccgc cggtggcggc gatgtcgagc tcttcgattg
     4501 atgagatccg ggacggcatc gccatgaccc actccgccca atccggaccg agccccgcca
     4561 agcgccccat ccgagtgctg atcgtcgatg attccgcact ggtgcgcagc ctgctgacga
     4621 gcatcctgcg aacggacccg ggcatcgagg tcgtgggggt tgcgggggat gctcacgtgg
     4681 cgcgcgagaa gatcaaactt ctgcagccgg acgttctgac cctcgatgtg gagatgccgc
     4741 ggatggacgg catcaccttt ctccgaaatc tcatgcgcct gaggccgatg cccgtcgtca
     4801 tggtctcatc gctcaccgag cgcggcgccg atgtcacgct ggatgcgctg gcactcgggg
     4861 cggtggacta tctgaccaag cccaggatcg atctggccgc caccctcaag gattacagcg
     4921 aggagctgat cgagaagatc cgcaccgccg cgagagccag cgttcgggcc ctggagcccc
     4981 tgcgtgctat acggccaacc gcgaatctca cggcgatact gccgcaactg ccccgcgcca
     5041 gatcgctgcg gaccacggac cgcttcgtag ccattggcgc atccaccggt ggcaccgagg
     5101 cgatcaagga agtgctcacg aggttgccgg cggattctcc gggagttgtg atcacgcagc
     5161 acattcccaa ggccttcagc acacccttcg ccaagcggat gaacgactgc tgtcagttga
     5221 ccgtctacga agcggaggac ggtcagcaac tgcttgctgg gcacgcctac atcgcaccgg
     5281 gagaccgcca tctgatggtc gtgcgcgatg gcgcgcgata cgtcctgcga ctcgacgacg
     5341 gtctgccggt caaccgccat aagccgtcgg ttgacgtgct gttccgctcg gtcgcccaga
     5401 atgccggtcc caatgcgatc ggcgtcctgc tgaccggcat gggcaaggat ggggcccgcg
     5461 ggctgcggga aatgctcgat tcgggcagcc gcacgatcgc ccaggacgag gccagcagtg
     5521 tcgtgtgggg aatgcccggg gaggcggtcg ccctgggggc ggcccagcat gtcctgagcc
     5581 tggggacggt ggcggcaaag atcctggccc tggccgaatc gatggatctc ggcggccaaa
     5641 agcgcgagcc gtgatgcact gcccggacat aagtccccac aaacggagag gattgagggg
     5701 ttactgatgc gagaccgcga tgaataaagc ctctgcacag cctgacggcg cacccggcga
     5761 cccgcgcgcc tttgccttcg tgcaacagct cgccagcgag ctctccaagg gcaaggtcga
     5821 tttaccgagc ttcccggacg tggcgctgcg cgtgcgcaaa gtcctgtccg acgagaacgt
     5881 ctcccaggac aaggtcgtcc gggtcgtcgg atccgagccc gccctggccg cacggttgat
     5941 gcagatcgcg aactcggccg cgctcaattt cagcggcaag ccaatcaccg agctgcgaac
     6001 agcgatcgcg cgcatggggt tcaacatggt gcgcagcgcg gccatcgcat tcgccatgtc
     6061 gcagctgaag aaagtggact ccctgaaagg gctggaggcg ccgctggagg acctgtggaa
     6121 gcgcagtgcc accgtcgcag cgatgtcgca tgccgtcgca cggcgcttca gccgcgtcaa
     6181 cccggacacc gcgttgctgg cagggctgct gcacggcatc ggtcagcttt atatcctgac
     6241 tcgctcgagt cagcatcctg gcctgttcga gaaccaggtt gcgtacgccg gcatcgtccg
     6301 cgattggcac agtgcgatcg ccaaggctct gctggagaac tgggacatga cggaagagat
     6361 cgtggaagcc gtcagcaatt acgaggacct ggagcgcgtc cacagcgggc cggtggacct
     6421 caccgacgtg ctcacagtcg gtaatctgct ggccgccttc aagagtcatc ccgagaacat
     6481 cgagctcaac atgcacgatg tggctgcgtg caagcgcatg caggcggacc gggccgccta
     6541 cgaggagctc atcaaggaat ccgagcacga gatcgacaac ctgctgcagg cgcttgggac
     6601 ctgagccgcg gcacgcggag taaaccatga gtgtggcaca atcaacttca cgcacggccg
     6661 agcagcagga agccactcaa ggaagcgtcg ccttcaattt cgtccaggcc ctggccgccg
     6721 agctctcctc gggcaagatc gagctgccgg gcttcccgca catcgtcatg cgggtacaga
     6781 aaatcctcgc cgatgagaac gcggacacct cgcgcatcgt gcgggtgctg ggcagcgagc
     6841 cggtgctcgc ctcgcagatc gtctgcatgg ccaactccgc cgccctcaac cctggcggcg
     6901 cagtggtcgc cgacctgcgc gccgcggtgg cacgggtcgg actggatacg gtgcgctcgg
     6961 cgacgatctc tttcgctgtg cgtcagcttc acgatgcgcc cctgctgaaa ggcctggagg
     7021 cgccgctcgg agtgctctgg cgacgcagcg tgctggtcgc cagcctgagc tgcgtgatcg
     7081 cccgtcgtct gaccagcgtc aatcctgaca cggccctgct cgcaggcctg ctgcagggta
     7141 tcggccgcct ttacatcctg acccgggcca gcgggcatcg caccctgttc gccgaccagg
     7201 atatctaccg cacgatcgag caggattggc atctcagcat cgccgcggcg ctgctggaga
     7261 actggggcat tgccgaggag atcgtccagg cggtgcagga gtcggagaac ctcgagcgcg
     7321 aggcccgcgg cctgctttct ctcacggacg tgctggtcgc cgccacgatc ctggccgagc
     7381 accacaatca acctcaggca ctgtacgcgc agatgcactg tgcccggccg ctgcagcgat
     7441 tgaagctcga tgcgagcgcc tgcgagaagt tcatttccga atcggcgcag gacatcgagt
     7501 ccctgcgcga agcgctgggc tgaagccgca cgacagctga gcgcgcgggc aggaagcccg
     7561 cccgggcgag tgggcagccc tcagaccgct cccaggattc gcttggattg cccggctgca
     7621 gagtagccgc gcggtccgta gatgacggtc atgttggact cgcctctgag cacggccaga
     7681 cgtcgctcga cgtgcctgag catcgccgtc accagggccc cgtttctgtc attcagattg
     7741 cggcagcgca gggcgcgctg tgcacagtcg ctcagggtcg gcagaagcga gccgtccggg
     7801 tcacaccagc tcatgagcgc ctcgagtccg ggcttgtcgt ccgagtgccc atgcaggcga
     7861 cagagcgcgc gacgctgctc ctcgatttgt gccagcgagc cgagctgctc ctggcgagtg
     7921 cgggccgtgc tctggatctt ctccatgtcc tgcgcggcga gcgcctgatg ctcgcgctcg
     7981 agaagcgc
//
LOCUS       DASRHN010000290         8573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_431059, whole genome shotgun sequence.
ACCESSION   DASRHN010000290 DASRHN010000000
VERSION     DASRHN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431059"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..83)
                     /locus_tag="VFB37_11680"
     tRNA            complement(2..83)
                     /locus_tag="VFB37_11680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Leu,seq:tag)
     gene            471..953
                     /locus_tag="VFB37_11685"
     CDS             471..953
                     /locus_tag="VFB37_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO23152.1"
                     /translation="MADRRTRQASSGFALRLREVIEAHGSANSIATHIDRSEGAVRKW
                     LRGESEPNVTDLRAVCELTGTSIEWLASGRGPRDTASVSAAGQERSPPPSAEAAGKPL
                     NRPRLEAVLEALDAEIGRQGIQLNPAKRSLLIVTLYELCLESQKVDPQAVARLLRLAG
                     "
     gene            complement(950..1486)
                     /gene="mobB"
                     /locus_tag="VFB37_11690"
     CDS             complement(950..1486)
                     /gene="mobB"
                     /locus_tag="VFB37_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HZO23153.1"
                     /translation="MSEARVLGIAGWSGSGKTTLITKVIPLLANGGVRIATIKHAHES
                     FEVDQPGKDSFMHRKAGASEVIVSSPRRWVQMHELGEQPEVTLPELLQRVSACELILV
                     EGFKSRQHPKLEVFRRSLGKPPLYPEDSHIVAVASDSELRGAAVPVVDLNDLEAIAKM
                     IQRQARPLAEVLGRLSSR"
     gene            complement(1516..2511)
                     /locus_tag="VFB37_11695"
     CDS             complement(1516..2511)
                     /locus_tag="VFB37_11695"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZO23154.1"
                     /translation="MKICVFGAGAIGGYLAVELALAGNDVCLVARGPHLRAIQQNGLK
                     LLLHGQERVAHLAATDDPASLGVQDFVICTLKAHQAYESAVAFAPLLGRSTAIVTAMN
                     GIPWWYFYRAGGRLEGHHLMAVDPGGHQWKTLGPQRAIGCVVDPACHLQAPGVIVHQE
                     FNRFTLGEPDGSRSERVLALSRTLSEAGFDAPVRDNVRWNVWLKLWGNVCFNPISALT
                     GATLDRITGDPGLRALCTTMMLETRAVNEALGIQIPLEMLERRLNAAGSVVGHKMSML
                     QDLEAGKPMEIDALVTAVQEMGRLTAVPTPCIDAVLALVLARARGLGLYAPLAAS"
     gene            complement(2535..3437)
                     /locus_tag="VFB37_11700"
     CDS             complement(2535..3437)
                     /locus_tag="VFB37_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO23155.1"
                     /translation="MFIRQLEYLAALAREKHFVKAAEVCHVSQPALSSAIRRLEEELD
                     IMIVQRGRRFVGLTVEGERVLAWARQTLASLSHMREEVSFARSAVAGTLRIGAIPTTM
                     TVAALLTSACRAAHPNIRYTLTSLSTDKIVSQLDHFELDVGLTYLDYELMDGFDKLHL
                     FNERYVLVASIHEKLEHTMTWKHAAQLPLCLLTGKMRNRQVIDAAFRREGAQPEVILE
                     TDSLFSLYAHVSEAGLFSIMPHSLLSFLDLTNRVQAKPLVPELTRGIGLLARNRPSLA
                     PITAAMWELARGLQLQSRFDRIIV"
     gene            3503..4480
                     /locus_tag="VFB37_11705"
     CDS             3503..4480
                     /locus_tag="VFB37_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZO23156.1"
                     /translation="MAVVQIASPGGPEQLQAATRPLPKPAEGEVLVRVAAAGINRPDV
                     MQRQGKYPAPPGASDLPGLEIAGEIAALGPKVTGLSVGDPVTALLAGGGYASYAVASA
                     ALCLPVPSGLSMVQAAAIPETYFTVWTNLFERGRCAAGDNVLVHGGTSGIGTTAIQLA
                     SAWGARVFATAGSEEKVRACEKLGAVRGINYRTEDFVEVTRRETGGKGVDVILDMVGG
                     SYLERNLEAAAMEGRIVCIATLGGTRAELKMVPLMQKRLTLTGSTLRARTVQQKAAVA
                     AAVRANVWPLLEAGRARPVIHATFPLAQASEAHRLMETSSHIGKIVLTV"
     gene            complement(4642..5574)
                     /locus_tag="VFB37_11710"
     CDS             complement(4642..5574)
                     /locus_tag="VFB37_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZO23157.1"
                     /translation="MTTAYITHGDCLRHEMGAGHPESPERLASINEHMRASGLLDTVR
                     SLEAPLAEEEDLARVHRPEYVELIFENAPEEGYLQLDPDTAMNPLSLAAARRAAGAGV
                     LAVTETIEGRVGNAFCAVRPCGHHATQRRAMGFCIFNNIAVAAAYALEQMGLERVAII
                     DFDVHHGNGTEDMFSAPQWQERVLMASFFQHPFYPYSGTDHPAPNMINVPLAAGSDGT
                     RARKAVQEHWLPALGRFKPQMILISAGFDAHRDDLLGGLALIEDDYVWMTRELMGVAA
                     QHAQGRIVSMLEGGYNLAALGRSAVAHVRALSDS"
     gene            complement(5603..6535)
                     /locus_tag="VFB37_11715"
     CDS             complement(5603..6535)
                     /locus_tag="VFB37_11715"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZO23158.1"
                     /translation="MRIALVGQQDFGKAVLEAFVSRGDTVVGVFCAPEKPGARPDPLR
                     LAAQERGVPVFQAPSLKGEEARQSLQALNADLGVMAYVLQFVPQEFVAVPRHGMIQYH
                     PSLLPRYRGPSSINWPIIRGEAETGLTIFRPTDGLDEGPIILQREVSIGPDDTLGSVY
                     FERLFPLGVAALLEASQLVLSGSARPVEQEESQASYEGWCRSAEAQVHWHNHVDLIYN
                     LIRGCNPAPGAWTAFRGKSLQLFDARKHPFRRFSQVSGDIGTVTDIRAESVFVTAQGG
                     QIEVLKVKYQDGKKISAAQFCAESGLSVGTKLGT"
     gene            6668..7876
                     /locus_tag="VFB37_11720"
     CDS             6668..7876
                     /locus_tag="VFB37_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZO23159.1"
                     /translation="MAGRNFLFVPGPTNVPDRILRAMSVAMEDHRSPTFPELTFTCLS
                     GLKPVFKTTTGTPFVFPSSGTGCWEAALTNCLNPGDKVLAARFGQFSHLWIEMCQRLG
                     YEVEILETPWGEGAPVERYLASLSADKQRKIKAVLVCQNETATGVTSDVAAIRRAMDE
                     AKHPALLFVDSVSALVSIDFRMDEWGVDVCVSGSQKGFMLPAGMGITCVSQKALTAAK
                     SAKSPRCYFDYADMVSANATGYFPYTPSVPMMYGLRESLAMIAEEGLENIFKRHSYLA
                     SGVRAAILEGWKLKLCATEPKWYSDTVSAVMVPEGINASEIIARAFKRYNLALGAGLS
                     KVAGKLFRIGHLGDLNELMLLGCLAGAEMAMLDSGVRVQPGSATAAAQEYWRSHAPAA
                     GASAKQSRAA"
     gene            7873..>8573
                     /locus_tag="VFB37_11725"
     CDS             7873..>8573
                     /locus_tag="VFB37_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO23160.1"
                     /translation="MSQRVVFLDRASLKARVRKPSCASEYVEYDKTATQEIVPRLKSA
                     TVAIINKVTLDARTLEQLPELKLIAVAATGYDVVDVEYCKRRGISVTNIRNYAIHTVP
                     EHAFALILALRRNLLAYRQDVENGVWNRCEQFCFFTHPIGDLYGSTLGIIGEGAIGQA
                     TAAIGKAFGMRVLFADHPPPKLDGVIFTPHEQVLAESDVISLHCPLMPATRNLINLEA
                     LRRMKRNALLINTARG"
BASE COUNT         1556 a         2785 c         2739 g         1493 t
ORIGIN      
        1 atgcgaaagg agagactcga actctcacgg gtttccccac tggaacctaa atccagcgcg
       61 tctaccagtt ccgccacttt cgcccgtgca gcgcgcgcac cgaacgtgcg gcttcgggca
      121 gcgctattcg actgatgctg gcgtactttt ggggcccggc gagtataccc gatgagcggc
      181 ccagccacgt ccgctggcgc agccgcaagt taggcattgc cctgggcaag gagattctga
      241 cgccgggtcg tgcccctgtt cacccggagc ggcagcacag cgacgcgact gcaccggagt
      301 acgtccactc gagcgcgtga cccagctacc ttccggctcc ggggaaaaca cccgcgctct
      361 tttgaccatc cgcagctcct cccgcttgcg gagcccgcgc agagtacgtt actttaatac
      421 gaatcttcgt cacttatatg acggacgcaa cgccttccac gcgcgatcgc atggcggaca
      481 gacggaccag acaagcgagc agcggtttcg ccctgcgact gcgcgaggtg atcgaggcgc
      541 atgggagtgc caattcgatc gccacgcaca tcgaccgttc agagggcgcg gtgcgcaaat
      601 ggctccgcgg ggaatccgag cccaacgtca ctgacctgcg agcagtttgc gagctgaccg
      661 gcaccagtat cgaatggctg gcaagtggac gcggccctcg cgacacggcc agcgtgtcag
      721 cggcaggaca agagaggtcc ccgccgccct ctgcagaggc tgccggcaag cccctcaacc
      781 gcccgcggct cgaagccgtc ctggaagctc tggatgcgga aatcggccgg caggggattc
      841 aactcaaccc ggccaagcga tcgctgctga tcgtcacgct gtacgagctc tgcctggaat
      901 cgcagaaagt ggatccgcaa gccgtcgcgc gtttgcttcg attggcgggt tagcgggagc
      961 tcagtctccc caacacttcc gccagcggac gcgcctggcg ctggatcatt ttcgcaatgg
     1021 cctcgagatc gttcaggtcg acgactggaa cagccgcccc gcggagctcg ctgtcacttg
     1081 cgacggcaac gatgtgtgaa tcctcgggat agagtggcgg cttgccgagg ctgcgccgga
     1141 agacttccag cttggggtgt tggcggctct tgaagccttc gaccaggatc agctcgcagg
     1201 ccgagacccg ctgcagcagc tcgggcaacg tgacttcagg ctgctctcca agctcatgca
     1261 tctggaccca gcggcgtggg gaggagacga tgacttcaga ggcgccggcc ttgcgatgca
     1321 tgaaggagtc cttgcccggc tgatcgacct cgaaagactc gtgcgcgtgt ttgatggtgg
     1381 caatgcgcac gccgccgttc gccagcagcg ggatcacttt ggtgatgagg gtggtctttc
     1441 cggagccact ccagccagcg attcccagaa cgcgtgcttc gctcatgagg cggccccctt
     1501 tcacgcagac gccgatcagc tcgctgccag tggcgcgtac agtcccagcc cgcgggcgcg
     1561 cgccagcacc agagccagta cggcatcgat gcagggtgtc ggcactgccg tgagacgccc
     1621 catctcctgc accgcggtga ccagcgcatc gatctccatc ggtttgcctg cttcgaggtc
     1681 ctgcaacatg gacatcttgt ggccgacgac ggatcctgcc gcgttgagcc gtcgttccaa
     1741 catctccagc gggatctgga tcccgagcgc ctcattcacc gcgcgggtct ccagcatcat
     1801 ggtcgtgcac aacgcccgca gcccgggatc cccggtgatg cggtccagcg tggcgccggt
     1861 gagcgcgctg atcggattga agcagacatt gccccagagt ttgagccaga cgttccagcg
     1921 tacgttgtcg cgcacgggcg catcgaaacc cgcctcgctc agtgtcctgc tgagcgccag
     1981 gacgcgctcg gaccggctgc catcgggctc tcccagcgtg aatcggttga attcctgatg
     2041 cacgatgacc ccaggtgcct gcagatgaca ggccgggtcc acgacacagc cgatcgcacg
     2101 ctgcggcccg agggttttcc attgatgccc gcccggatcc acggccatca gatggtggcc
     2161 ctccagtcgg ccgcccgccc ggtagaaata ccaccaggga attccgttca tcgcggtgac
     2221 gatcgccgtg gaacggccca gcagcggggc gaacgcgaca gccgactcgt acgcctggtg
     2281 tgccttgagc gtgcagatca cgaagtcctg cacgcccagg cttgccggat cgtcggtcgc
     2341 ggccaggtgc gcgacgcgct cctgtccatg caacagcagt ttcaaaccgt tctgttgaat
     2401 cgcgcgcagg tgcggacctc gcgccacgag gcagacgtca ttgccggcca gcgccagctc
     2461 gaccgcgagg tagccgccga tggcgccggc gccaaagaca cagattttca tgcagcaccc
     2521 cgcaagagca caccctacac gatgatccga tcgaagcggg actgcagctg caggcctcgc
     2581 gccagctccc acattgccgc cgtgatcggc gccagagacg gccggttgcg cgccagcaac
     2641 ccgatgcctc gggtcaactc cggcaccagg ggtttggcct gcacccggtt cgtcagatcg
     2701 agaaagctca gcaggctgtg cggcatgatg ctgaacaacc cggcctcgct cacgtgcgca
     2761 tagagggaaa agagcgaatc ggtttcgaga atgacctccg gctgcgcacc ctcgcgtctg
     2821 aaggcggcat cgatcacctg ccggttgcgc atcttgcccg tcagcaggca caggggcagc
     2881 tgcgcggcat gcttccaggt catcgtgtgc tcgagcttct cgtgtatgga agccacgaga
     2941 acgtagcgct cgttgaacag atgcaacttg tcgaagccgt ccatcagttc atagtcgaga
     3001 taagtgaggc ccacatcgag ctcgaagtga tcgagctggc tgacgatctt gtcggtgctg
     3061 agcgaggtga gcgtatagcg gatgttcgga tgcgcggcac ggcaggcgga ggtcagcagc
     3121 gccgcaacgg tcatggtggt cggaatcgcg ccgatgcgca gtgtgccggc caccgcggaa
     3181 cgggcgaacg acacttcctc gcgcatgtgc gatagcgagg cgagcgtctg ccgagcccac
     3241 gccagcactc gttcgccttc gacggtcagc ccgacgaagc gccgcccacg ctgcacgatc
     3301 atgatgtcca gctcttcctc gagccggcgg atggccgatg acagcgccgg ctgggagaca
     3361 tggcagacct cggccgcctt cacgaagtgc ttctcgcgcg cgagcgcggc aaggtattca
     3421 agttgccgga tgaacatgtt agattggaac tggtaacaga gagcgtgttc ggagagcgat
     3481 catttcagcc ctgcctgtcg agatggccgt cgtgcagatc gcctcgcccg gtggcccgga
     3541 gcagctgcag gccgccacgc gtcccctgcc gaagccggcg gagggtgaag tcctcgtgcg
     3601 cgtcgcggct gcgggaatca accgccccga tgtgatgcag cgccagggaa aatatccggc
     3661 gcctcccggc gcttccgacc tgcctgggct ggaaatcgcc ggcgagatcg ccgcgctcgg
     3721 gccgaaggta acgggcctgt cggtgggcga tccggtgaca gccttgctgg ccggcggcgg
     3781 atatgcgagc tatgcggtgg cctccgctgc gctgtgcctg ccggtgccca gcggcctgag
     3841 catggtgcag gctgccgcaa tacccgaaac gtatttcacc gtctggacga accttttcga
     3901 acgaggccgc tgtgctgccg gggataacgt gctggtgcac gggggaacca gcggcatcgg
     3961 taccacggcg atccaactcg cgagcgcctg gggagctcgg gtgtttgcca ccgcaggctc
     4021 cgaggagaaa gtgcgcgcgt gcgagaaact cggagccgtc cgcggaatca actatcgcac
     4081 ggaggatttc gtcgaggtca cgcggcgcga aaccggcggt aaaggcgtcg atgtgattct
     4141 ggatatggtc ggcggcagct acctcgagcg aaatctggag gccgcagcca tggagggtcg
     4201 gatcgtctgc atcgcgacgc tcggcggcac ccgggcagag ctcaagatgg tgcccctcat
     4261 gcagaaacgc ctgaccttga ccggctcgac gttgcgcgcc cgcacggtgc aacagaaggc
     4321 cgcggtggcc gccgccgttc gagcgaacgt ctggcccctg ctggaagcgg gccgcgcgcg
     4381 gcccgtgatt catgcgacgt ttccgctggc gcaggcaagc gaggcgcatc gtctgatgga
     4441 aacctcgagc cacatcggca agatcgtgct cacggtttga tcgaatagcc tgggcaggca
     4501 ccccgcgtcg ggtggcgccg cgccacgcgc agtcccgcaa cgcagcgcca agcccctgcg
     4561 caacgcaggc cctttccggc ggcgccacgc tcgccggagt tccgcgacga ccactggcca
     4621 ccgcgccccg gcgacggcgc ctcatgaatc gctcagcgcc cggacatggg ccacggcgct
     4681 gcgacccagg gccgcgaggt tatagccgcc ctcgagcatc gagacgatcc gtccctgcgc
     4741 gtgctgtgcc gccactccca tcagctcacg ggtcatccac acgtaatcgt cttcgatgag
     4801 cgcgagaccg cccagcaaat catcgcgatg cgcatcgaat ccggccgaga tgaggatcat
     4861 ctgcggcttg aagcgcccga gcgccggcaa ccagtgctcc tgcaccgcct tgcgggccct
     4921 cgtgccatca ctgcctgcgg cgaggggcac attgatcatg ttgggggcgg gatgatccgt
     4981 cccgctatac ggatagaacg gatgctggaa gaagctcgcc atcaacacgc gctcttgcca
     5041 ttgcggggca ctgaacatgt cctcggtgcc attgccgtga tgcacatcga agtcgatgat
     5101 cgccacgcgc tccaggccca tttgctccag cgcgtacgca gcggccacgg cgatgttgtt
     5161 gaagatgcag aaccccatgg cccgacgttg cgttgcgtgg tgtccacacg ggcgcactgc
     5221 gcagaaggcg ttgccgacgc ggccctcaat cgtctccgtc acggccagaa cgcctgcacc
     5281 ggccgctctg cgagccgccg cgagactcag cggattcatg gccgtatcgg gatcgagctg
     5341 caggtaacct tcctcgggag cattctcgaa gatcagctcg acgtattccg gacggtggac
     5401 gcgcgcgagg tcctcctcct cggccagggg agcctcgaga ctgcgcaccg tatccaacag
     5461 tccggaagct cgcatgtgct cgttgatgga ggcgaggcgt tcgggacttt cggggtggcc
     5521 cgctcccatc tcgtgccgca gacagtcgcc atgggtgatg taggcagtag tcatttgcat
     5581 ctcctgcagc ggagccgccg gatcaggtcc ccagtttggt gccgacggag agccccgact
     5641 cggcgcagaa ctgcgccgcg gagatcttct ttccgtcctg atacttcact ttgagcactt
     5701 cgatctggcc tccctgggcg gtgacgaaca cgctctccgc acggatgtcc gtgacggttc
     5761 cgatatcgcc cgagacctgc gagaacctgc gaaacgggtg cttccgggca tcgaacagtt
     5821 gcaggctctt gcctcggaaa gcggtccagg cgcccggcgc cggattgcag ccgcgaatca
     5881 ggttgtagat cagatcgacg tggttgtgcc agtggacctg cgcttcggcg ctccggcacc
     5941 aaccctcgta actcgcctgg gactcctcct gctcgacggg gcgagcgctc ccgctgagca
     6001 ccagctgcga agcctcgagc agagcagcca cgccgagggg gaaaagtctt tcgaaataca
     6061 cgctgccgag cgtgtcatcc gggccgatcg acacctcgcg ctgcaggatg atcggaccct
     6121 catcgagacc gtcggttgga cgaaagatcg tcagaccggt ctcggcctcc ccgcggatga
     6181 tcggccagtt gatcgagctc gggccgcgat agcgcggcag cagcgagggg tggtactgga
     6241 tcattccgtg ccggggcact gcaacaaact cctgcggcac gaactgcagg acgtaggcca
     6301 tcacgcccag gtccgcattc agggcctgca aggattggcg agcctcctca cccttcagtg
     6361 agggggcctg aaacaccggg acgccgcgct cctgggcggc cagacgcagc ggatccgggc
     6421 gggcgcccgg cttttcgggg gcgcagaaaa cacccacaac ggtgtcgccg cggctcacga
     6481 aagcctcgag cacggccttg ccgaaatcct gctgccctac gagggcgata cgcatcgctc
     6541 aacctcccgg tcggagccgg tgccggcgca tggggcgccg gagcgacttc ccgcccagat
     6601 tatatgtaca ccttctcata tctcatatat gttgtaccga caagcttccc cgtcttgagg
     6661 atctcccatg gctggtcgca atttcctgtt cgttcctgga cccacgaacg tgcctgatcg
     6721 cattctgcgt gccatgagcg tcgccatgga ggatcaccgc tcacccactt tccccgagct
     6781 caccttcact tgcctgagtg ggctgaagcc ggtcttcaag accacgaccg gcacgccttt
     6841 tgtgtttccc tcttcgggca ccggatgctg ggaggcggca ctcaccaact gcctgaatcc
     6901 gggggacaag gtcctggcgg ctcgcttcgg gcagttcagc cacctgtgga tagagatgtg
     6961 tcagcggctc ggctacgagg tcgagatcct cgaaaccccc tggggagaag gcgctccggt
     7021 cgagcgctac ctggcatcgc tcagcgccga caagcagcga aagatcaagg ccgttctggt
     7081 ttgccagaac gagacggcca cgggggtcac cagcgatgtt gccgcgatcc ggcgcgcaat
     7141 ggacgaggcc aaacatcccg cgctgctgtt cgtggactcg gtgagcgcac tggtgagcat
     7201 cgactttcgc atggatgagt ggggcgttga cgtttgcgtc agcggctcgc agaagggctt
     7261 catgctgccg gccggcatgg gcattacgtg cgtgagccag aaagccctca ccgcggcgaa
     7321 gagcgcgaag tcgccgcgct gttatttcga ttatgcggac atggtgagtg cgaacgcgac
     7381 gggctatttc ccctacaccc cttccgtgcc gatgatgtac ggcctgcgcg agtcgctcgc
     7441 catgatcgcc gaggaggggc tggagaacat cttcaagcgc cacagctatc tcgcctccgg
     7501 cgtgcgcgcg gcgatactcg agggctggaa actgaagctg tgcgcaaccg agccgaaatg
     7561 gtattccgat accgtctcgg ccgtcatggt gcccgaaggc atcaacgcat cggaaatcat
     7621 cgcgcgcgcg ttcaagcgct acaacctggc actcggtgcc ggcctttcga aggtcgccgg
     7681 caagctgttc cgcatcggtc atctgggcga tctcaatgag ctgatgctgc tgggctgcct
     7741 ggccggcgcc gagatggcga tgctcgactc gggcgtgcgc gtgcagccgg gcagtgccac
     7801 ggcggctgcg caggaatact ggcgcagcca tgcccctgcg gcgggcgcca gcgcgaagca
     7861 gagccgggcc gcgtgagcca gcgggtcgta ttcctggatc gcgcctctct caaagcgcgc
     7921 gttcgcaagc cctcttgcgc gagcgagtac gtggaatacg acaagacggc gacccaggag
     7981 atcgtgccgc ggttgaagag cgcaaccgtg gcgatcatca acaaggtcac cctggatgcc
     8041 agaacgctcg agcagctccc tgagctgaag ctgattgcgg tcgctgcgac gggctacgac
     8101 gtcgtcgacg tcgaatattg caagcggcgg ggcatcagcg tcaccaacat ccgcaattac
     8161 gcgatccata cggtaccaga gcacgcgttc gcattgatcc tggccttgcg gcgcaacctg
     8221 ctagcttatc ggcaggatgt cgaaaacggg gtgtggaacc gctgtgagca gttctgtttc
     8281 ttcacccacc cgatcggaga tctttacggg tccacactcg gaatcatcgg cgagggtgcg
     8341 atcgggcagg cgacggccgc aatcggcaag gcattcggca tgcgagtcct gttcgccgat
     8401 catcccccgc cgaagctcga cggggtcatc ttcacgcccc atgagcaggt acttgccgaa
     8461 tccgacgtga tttcgcttca ttgcccgttg atgcctgcga ctcgcaatct gatcaacctc
     8521 gaggccttgc gccggatgaa gcgcaatgca ctgctcatca acaccgcgcg cgg
//
LOCUS       DASRHN010000291         7845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2079660, whole genome shotgun sequence.
ACCESSION   DASRHN010000291 DASRHN010000000
VERSION     DASRHN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079660"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFB37_11730"
     CDS             complement(<1..807)
                     /locus_tag="VFB37_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZO23161.1"
                     /translation="MRGARAVALLVLAALVVIAATSLFTISEAQLAIRTEFGAIVGLD
                     YAPGLHVKWPWDQVVKFDRRILSLSYTGETFLTNDNRGLIVDFYVKWRIKDASRYFQA
                     TGGREDLAGERLAEIVKDGIKSVVAQRTLEQIVSSERAAVTGDMFGQASRNVANLGIE
                     LVDVRVQRIDLPDDVSTRVYESMKQNFAKIASRLRAEGQSESVRLRSSAERQRTEILA
                     NAERDALRTRGEGDAQATQTYSKAYSKDPEFYAFYRSLQAYERSLGKDGDL"
     gene            complement(804..1934)
                     /gene="hflK"
                     /locus_tag="VFB37_11735"
     CDS             complement(804..1934)
                     /gene="hflK"
                     /locus_tag="VFB37_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZO23162.1"
                     /translation="MAWNQPGQNNPWGRRPGQGNGPDLEERVKGWQRRLESLLRPGGR
                     GSESGMLFLILALLVVAVWLASGFYQIKAAEQGVILRFGQLVRVRSQGLGWHLPWPIE
                     TLLKVNVASVNSSEYKSRVLTSDVNLVDLRFAVQYQLSDPIKALFRVKDPEATLSEVS
                     EMAIREIVGRSELDEVLVGATRPQITRRTKELIQHTLDTYNTGITVTTVNLTDVQVPE
                     AVVPSQRDANKALADQERYVKEAQAYANSIIPVAHGAASRMLQDAEAYKAQVTAIAEG
                     QAGRFSQLEQAYAQSPEVTRRRLYIDTVENVLSRAHKVLIDAKANGTSGGNMFYLPLD
                     KLLEKATSRGDSESGSGESSSNPPAPGREPDVTVEARSRGDR"
     gene            complement(1979..3328)
                     /gene="hflX"
                     /locus_tag="VFB37_11740"
     CDS             complement(1979..3328)
                     /gene="hflX"
                     /locus_tag="VFB37_11740"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF008280.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HZO23163.1"
                     /translation="MKPSERPRTGERAVLVRLGLGAPVDPEDLQELRQLALSAGALPV
                     ATLTGRRERPDPRFFLGSGKAQELRDVALANEADIVLVDHALSPSQERNLEKLAGRRV
                     LDRNGLILDIFAQRARSFEGKLQVELAQLKHLSTRLIRGWTHLERQKGGIGMRGPGET
                     QLETDRRLIGQRVRLLTKRLARISQQRETGRQMRAEIPVPSLALVGYTNAGKSTLFRA
                     VTGADAYVADQLFATLDPTVRRITLPGGTPAVMADTVGFIRELPHELVAAFRSTLTEA
                     REATLLLHVVDASDPRREEHMAQVDTVLAEIGADTIPQLLVYNKIDRLALAPRLDRDA
                     NGRAAAVWISAERRTGLELLAEAISERLARFARQARIRVPASAGALRSRLYAARAVRE
                     ECSSQDGSIDLSVELPEVELLALARVPGVQILEVKAGDTACAPDNRYLQSTAVSQLR"
     gene            complement(3333..3590)
                     /gene="hfq"
                     /locus_tag="VFB37_11745"
     CDS             complement(3333..3590)
                     /gene="hfq"
                     /locus_tag="VFB37_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZO23164.1"
                     /translation="MAKGQSLQDPFLNALRKERVPVSIYLVNGIKLQGQIDSFDQFVV
                     LLKNSVSQMVYKHAISTVVPARNVRLAAEEGGPAAETNPAE"
     gene            complement(3735..4697)
                     /gene="miaA"
                     /locus_tag="VFB37_11750"
     CDS             complement(3735..4697)
                     /gene="miaA"
                     /locus_tag="VFB37_11750"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZO23165.1"
                     /translation="MSRGAAADPLPVWLLAGPTGVGKSEWALQLAESAPIEIVSVDSA
                     LVYRGLDIGSAKPARGLRERIAHHLIDICDPAESYSAGRFLADALAAIPAIHARGRVP
                     LLVGGTMLYFRALLHGLAPLPEATPDLRRKIDERAAREGWIALHAELALCDPAAAARI
                     NSSDAQRIQRALEVCYASGRPISELQLHTVSPLAGMPVRAWALIPSDRAQLHRHLEKR
                     FHAMMAQGFLEEVRALHERADLHARHSSMRAVGYRQLWAHLEGEYELQEAIRRSVSAT
                     RQLAKRQLTWLRREHAFESLDAQDSHTRASWIGDAQSALRELGL"
     gene            complement(4697..6475)
                     /gene="mutL"
                     /locus_tag="VFB37_11755"
     CDS             complement(4697..6475)
                     /gene="mutL"
                     /locus_tag="VFB37_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZO23166.1"
                     /translation="MTIRVLPNELIDQIAAGEVIERPASVVKELVENSLDSGARRIEI
                     DIERGGVGLVRVRDDGCGIAAAELHVAVSRHATSKIGSLEDLEAITTLGFRGEALPSI
                     GSVSRQRVVSRVEGAPAGAEISVDGGKVSAIRPAAHPAGTTVEVHDLFFNVPARRKFV
                     RSDATELGHIARLVERLVLSRHDVTFRLRSGERVLIEAPAGAEDDSRRLARILGEGFP
                     ARSVPVRHHAGPVEIAGWIGLPTTSRAQPDQQFWFVNGRSVRDKLLMNAVRVAYRDVL
                     YHGRYPTYVLYLTLDPKLVDVNAHPSKLEVRFRDSRQIHEFVFRALERVLAGTRPDFA
                     AAPAAQADFPPYRESTLPFPTSSRSVWGVIGAVREPEASTREMSGGGRWASGGAGFAN
                     ASSEEAEHPLGVALAQLHGVYILAQTPEGLVLVDMHAAHERVLYERFKAANGAATPAS
                     QQLLEPIVIDLKPHEVEAILEGRAEWERAGFELDAVGPARLALRRIPALLVGEDVAGI
                     VASVVRELEAEAGTHHLEAAADRFLGTLACRTAIHANRRLSLAEMNALLRQMESTARA
                     NQCNHGRPTWARLSLAELDQLFLRGR"
     gene            complement(6563..7768)
                     /locus_tag="VFB37_11760"
     CDS             complement(6563..7768)
                     /locus_tag="VFB37_11760"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZO23167.1"
                     /translation="MFKLGAGLIALSAAGAALAAGAELHGVHLSGAAHSAQLRLDVTG
                     LATKRIFTLERPFRTVIDLSQTRVARGLRMPAGAGLVSDIRLGQQPGGTSRIVVQLSE
                     HLIAHSIWLAASQPGHRELVIDFGDPPAQADTVAHIVRAAHAPVDSDRDVIIAVDAGH
                     GGADPGAIGRSGTREKDVVLAIARLLARRIDEEPGMKAVLTRDRDEFLVLRDRIRRAR
                     VAKADMFVSVHADSIANRDISGASVYVLSERGASNEAARWLAERENAADLMGGVSLED
                     KDATLKSVLLDLSQSANISASMSAAQRVLSALDGVGAVRKSQVQQAGFVVLKSPDIPS
                     MLVETAYISNPHEELRLRSVHQQVSLADAIFEGIRGYFERNPPAGSRFAAQLRGSTVA
                     SVIAAPSTR"
BASE COUNT         1339 a         2650 c         2510 g         1346 t
ORIGIN      
        1 cagatcccca tccttgccga gtgagcgctc gtaggcctgc aggctgcgat agaaagcgta
       61 gaactccgga tccttggaat aggctttcga gtaggtctgg gtcgcctgtg catcgccttc
      121 gccacgagtg cgcagcgcat cgcgctcggc attggcgagg atctccgtgc gctgacgctc
      181 cgcggaggag cgcagccgca cgctctcact ctggccttcg gctctgaggc ggctggcgat
      241 cttcgcaaaa ttctgcttca tgctctcgta gacgcgcgta cttacatcgt ccggcagatc
      301 gatccgctgt acgcgcacat ccaccagctc gattccgagg ttggccacgt tgcggctcgc
      361 ctgtccgaac atgtcgccgg tgaccgcggc gcgctcggag gagacgatct gctcgagggt
      421 gcgctgcgcg accacgctct tgatgccgtc cttgacgatc tcggccaggc gctcgccggc
      481 caggtcctcg cgtccgccgg tggcctgaaa gtagcgcgag gcatccttga tgcgccactt
      541 cacgtagaaa tccacgatca gcccgcgatt gtcgttggtc agaaaggtct cgcccgtgta
      601 cgaaagcgaa aggatgcgac ggtcgaactt cacgacctgg tcccacggcc acttcacatg
      661 caggccgggc gcgtagtcga ggcctacgat tgcgccgaac tcggtgcgga tggcgagctg
      721 cgcctcggag atcgtgaaca gcgacgtcgc tgcaatcacc acgagcgccg caagcaccag
      781 cagagccaca gcgcgcgcgc ccctcatcga tccccccggc tgcgcgcctc gacggtcaca
      841 tccggctcgc ggcccggcgc cggcgggttg ctcgaggatt ctccggagcc cgattcggaa
      901 tccccgcgcg aggtggcttt ctccagcagc ttgtcgagcg gcagatagaa catgttgccg
      961 ccactggtgc cgttggcctt cgcatcgatc aacaccttgt gggcgcgcga gagcacgttc
     1021 tccaccgtat cgatgtacag gcgccgccgt gtcacctcgg gtgactgtgc atacgcctgc
     1081 tcgagctgcg agaagcgtcc cgcctgacct tcggcaatgg ccgtgacctg ggccttgtac
     1141 gcctcggcat cctgcagcat gcgcgaggcc gccccgtggg ccacgggaat gatgctattg
     1201 gcgtaagcct gggcttcctt gacgtagcgc tcctggtcgg cgagtgcctt gttggcatcg
     1261 cgctgcgagg gcaccacggc ctccggcacc tgcacatccg tcagattgac ggtcgtgacg
     1321 gtgatgccag tgttgtatgt gtccagcgta tgctggatca gctctttcgt gcggcgcgtg
     1381 atctgcggtc gcgtcgcacc gaccaacacc tcatccagct cgctgcggcc cacgatctcg
     1441 cggatcgcca tctcgctgac ctcgctcagc gttgcctccg gatccttgac acggaacagc
     1501 gccttgatgg gatcgctgag ctggtactgc acggcgaagc gcagatcgac caggttcaca
     1561 tccgaggtga gcacgcgcga cttgtactcg ctcgagttga cgctcgcgac attgaccttc
     1621 agcagcgtct cgatcggcca gggcaggtgc cagcccaggc cctgcgagcg cacgcgcacc
     1681 agctgcccga accgcaggat caccccctgc tcggcggcct tgatctgata gaagccgctc
     1741 gccagccaga cagccacgac caacagagcg aggatcagga aaagcatgcc gctctcgctg
     1801 ccgcgcccgc ccggacgcag cagcgactcg aggcgccgtt gccagccctt cacgcgctcc
     1861 tcgaggtccg gcccgttgcc ctgacccggg cggcggcccc atggattgtt ctgacccggt
     1921 tgattccacg ccataagaca aattcgagct gcgcgctgcg cgcaggggtt aacggatctc
     1981 agcgcagctg cgagacggcg gtcgattgta ggtagcggtt atcgggcgca caagccgtgt
     2041 cccccgcctt cacctcgagg atctgcaccc ctggcacgcg tgccagggcg aggagctcga
     2101 cctcaggcag ctccacgctc agatcgatcg acccatcctg tgaggaacat tcctcacgca
     2161 cggcccgcgc cgcatacagc cgcgagcgca gcgcaccggc ggaggccggc acgcggattc
     2221 gtgcctggcg cgcgaagcgc gccagacgct cgctgatcgc ctcggccaac agctcgaggc
     2281 cggtgcgccg ctcggcggag atccacactg ccgcggcgcg cccatttgca tcccggtcca
     2341 ggcgcggcgc aagtgccagg cgatcgatct tgttgtacac gaggagctgc gggatcgtgt
     2401 cggcgccgat ctctgcgagc acggtatcga cctgcgccat gtgctcctcg cggcgtggat
     2461 cactcgcatc cactacgtgc aacagcaggg tcgcctcccg cgcttcggtc agagtggagc
     2521 ggaatgcagc cacgagctcg tgtggcagct cgcgaatgaa tcccacggta tcggccatca
     2581 cggcgggagt tccccccggc agcgtgatac gcctcaccgt tggatcgagt gtggcaaaaa
     2641 gttggtccgc gacataggca tcggcaccgg tcaccgcgcg gaacagcgtg gacttgccag
     2701 cattggtata ccccacgagc gcaagcgacg gcaccggtat ctcagcccgc atctgtcgtc
     2761 cggtctcacg ctgctggctg atccgcgcga gccgcttcgt cagcagccgt actcgctgtc
     2821 cgatcaggcg gcgatcggtc tcgagctgcg tttcgccggg gccgcgcatg ccgattccgc
     2881 ctttctgacg ctccagatgc gtccagccgc gtatgagccg cgtgctgaga tgtttgagct
     2941 gtgcgagctc gacttgcaac ttcccttcga agctgcgggc ccgttgcgcg aagatgtcga
     3001 gaatgaggcc gttccggtcg agcacccgcc tgccggcgag cttctccagg ttgcgctcct
     3061 ggctcggtga gagggcgtgg tccacgagca cgatgtcggc ctcattggcc agggcgacat
     3121 ccctgagctc ttgcgctttg ccgctgccca ggaaaaagcg cgggtccggc cgctcacggc
     3181 ggccggtgag cgtcgcgacc ggcaaggcac ctgcggaaag cgcgagttgc ctgagctcct
     3241 gcagatcctc gggatccacg ggcgcgccca gcccgagacg cacgagcaca gcacgctcac
     3301 cggtgcgggg gcgctcgctg ggtttcatgc gctcattcgg cgggattcgt ctccgcggcg
     3361 gggccgcctt cctcggccgc cagccgcacg ttgcgcgccg gcacgaccgt ggagatcgca
     3421 tgcttgtaaa ccatctgact cacactgttc ttcagcagaa cgacgaattg gtcgaacgag
     3481 tcgatctgac cctgcagttt gatgccattg acgaggtaga tcgaaacggg gacccgttcc
     3541 ttgcgcagtg cattcaggaa ggggtcttgc agggactggc ctttggccat cgggttctcc
     3601 ttgttttcca cacacagctt ctagttgttc gaaaaagcat cgcaagcgaa cttccctgtc
     3661 ccgcgatcaa gcaagctccg tgcctcgcca gtggcgagga ccgataggtt ttgcatctta
     3721 acatgcgttt tccgtcaaag cccaagctct cgcaacgcac tttgcgcgtc gccaatccac
     3781 gacgcccgag tgtgcgaatc ctgcgcatcg aggctttcga aagcgtgttc gcggcgtagc
     3841 caggtgagct gccgcttcgc aagctgtcgc gttgcggaaa cgctgcgccg gattgcctcc
     3901 tgcagctcat actcaccctc gaggtgagcc cagagctggc ggtaacccac ggcgcgcatg
     3961 gaggaatgcc gcgcatgcag gtcggcgcgt tcgtgcagcg cgcgcacttc ctccagaaaa
     4021 ccttgcgcca tcatggcgtg aaagcgtttt tcgaggtgtc ggtgcaactg cgcgcggtcg
     4081 gagggaatga gcgcccaggc acgcacgggc atcccggcaa gcgggctcac tgtatgcagt
     4141 tgcagctcgg agatcggccg gccgctcgcg tagcaaacct ccagagcgcg ctggatgcgc
     4201 tgggcgtcac tgctgttgat gcgcgcggcg gccgccgggt cgcacagcgc gagctcggca
     4261 tgcagcgcga tccaaccttc ccgggcggca cgctcgtcga tcttgcgacg caggtcaggc
     4321 gtcgcctcgg gcaggggcgc cagcccatgc agaagtgccc tgaaatagag catcgtgccc
     4381 cccaccagca acggcacccg gcctctggca tgaatggcgg gtatggcagc cagagcgtcg
     4441 gcaaggaatc gcccggcgga atagctctcc gccgggtcgc agatatcgat gagatgatga
     4501 gcaatacgct cccgcaatcc gcgcgcgggc ttggcactgc cgatgtccag gccgcgatac
     4561 accagcgccg agtccacgct cacgatctcg atgggggcgc tctcggccag ctgcagggcc
     4621 cattcgctct tgcctacccc ggtcggtccg gccagcagcc agaccggcag cgggtccgca
     4681 gccgcaccgc ggctcactag cgtccccgga gaaagagctg gtccagctcc gcaagcgata
     4741 gccgggccca ggtcggccgg ccgtgattgc attgattggc gcgcgccgtc gactccatct
     4801 gacgcagcag cgcgttcatt tccgcaaggc tcagtcggcg gttcgcatgg atcgccgtgc
     4861 ggcaggccag ggtgcccaga aaccggtccg ccgcagcctc gaggtgatgc gtccccgctt
     4921 ccgcctccag ctcacggacg accgaggcca cgatccccgc gacgtcttct ccgactagca
     4981 acgctgggat gcgcctcaac gcaagccgcg ccggccccac cgcgtcgagc tcgaaaccgg
     5041 cccgttccca ctcggcgcgt ccttccagga tcgcctccac ctcatggggc ttgagatcga
     5101 tcacgatggg ctcgagaagc tgctgcgagg caggggtcgc cgcaccgttg gcagccttga
     5161 agcgctcgta cagcacgcgc tcatgcgcgg cgtgcatatc cacgagcacg agaccctccg
     5221 gggtctgcgc gagtatgtaa acaccatgca gctgagcgag cgcgacaccc agcgggtgtt
     5281 ccgcttcctc ggaggaagca ttcgcaaacc ccgcaccccc ggaagcccac ctcccccccc
     5341 cggacatctc ccgggtggag gcttccggct cccgcacggc accgatgact ccccacacgc
     5401 tgcgggatga agtcggaaaa ggcagagtgg actcgcgata cggtggaaaa tctgcctggg
     5461 cggccggggc cgccgcgaag tccggacggg ttcccgcgag cacgcgttcc agcgcccgaa
     5521 atacgaactc gtgaatctgc cggctgtcgc ggaagcgcac ttcgagcttg gacggatggg
     5581 cgttcacatc gaccagtttc ggatccagtg tcaggtacag cacataggtc gggtagcgcc
     5641 cgtgatagag aacgtcgcgg taggcgaccc tgacggcgtt catcagcagt ttgtcgcgca
     5701 cactgcggcc attgacgaac cagaactgct gatccggctg cgctcgcgag gtggtgggga
     5761 ggccgatcca gcccgcgatc tccacggggc cggcgtggtg gcgtaccggg accgagcgcg
     5821 cgggaaatcc ctcacccagg atcctcgcca gccggcggct gtcatcctcg gctccggcgg
     5881 gcgcctcgat cagcacccgc tcgccacttc gcagtcgaaa ggtgacgtcg tgacgcgaaa
     5941 gcacgaggcg ctccaccaag cgtgcaatat gtcccagctc cgtcgcatca ctgcggacga
     6001 atttgcgccg cgccggcaca ttgaagaaca gatcgtgtac ttcgaccgtg gtccccgcgg
     6061 ggtgggctgc gggccggatc gcggagacct tgcccccatc gacgctgatc tcggcacccg
     6121 ccggtgcgcc ctcgactcgc gagacgactc gctgacggga caccgagccg atggagggaa
     6181 gcgcctcgcc acgaaatccc agcgtggtga tggcctcgag atcctcgagc gagccgatct
     6241 tgctcgtcgc atgccgcgaa accgccacgt gaagctcggc cgcggcgatg ccgcagccat
     6301 cgtcccgtac tctcaccaga ccgaccccgc cgcgctcgat gtcgatctcg atgcggcgcg
     6361 cgcccgagtc gaggctgttc tcgacgagct ccttcacaac cgaggccggc cgttcgatga
     6421 cctcgccggc agcgatctga tcgatgagct cgttgggcag cactcggatg gtcatttcgg
     6481 gtcagcgggc gcaggggcgc ggaaggcttc gcgcccagtg tagcgccagc ggatcagccg
     6541 gtgctcggcg cagtgcctgc gatcagcggg tgctcggcgc agcgatcacg ctggccacgg
     6601 tgctgccgcg gagctgggct gcgaaacggc ttcctgccgg gggattgcgc tcgaagtatc
     6661 cgcggatgcc ttcgaagatc gcatcggcaa ggctcacctg ctgatgcacg ctgcgcagac
     6721 gcagctcctc gtgcggattg gagatatagg ccgtctcgac cagcatggag gggatgtccg
     6781 gcgacttgag caccacgaat cccgcctgct gaacctggga cttgcgtacc gccccgacgc
     6841 cgtccagagc ggacagcacg cgctgggccg cgctcatgct ggcactgatg ttggcagatt
     6901 gcgaaaggtc gaggagtacc gacttgagtg tcgcgtcctt gtcctcgagg gagacacccc
     6961 ccatcaggtc cgccgcgttc tcacgctcgg cgagccaacg cgccgcctca ttcgaagctc
     7021 cacgttcgga caggacgtag accgaagcgc cggaaatgtc ccggtttgcg atggagtcgg
     7081 cgtgcacgga gacgaacatg tcggccttgg caactcgcgc ccgcctgatg cgatcgcgca
     7141 ggaccagaaa ctcgtcacga tcccgtgtca gcacggcttt catgccgggc tcttcgtcga
     7201 tccggcgggc cagcaagcgt gcgatggcga gcacgacatc tttctcccgc gtgccgctgc
     7261 ggccgatggc tccgggatcc gcaccgccgt ggcctgcatc gacggcgatg atcacgtcgc
     7321 gatcggaatc tacgggcgcg tgggcggccc gaacgatgtg tgcgaccgta tccgcctgcg
     7381 caggcggatc accaaagtcg atcaccagtt cccggtgccc gggctgggag gccgcaagcc
     7441 agatgctatg cgcaatcagg tgctcgctca gctgaacgac aattctggac gtgccgcccg
     7501 gctgctgccc caagcggatg tccgatacca gaccggcgcc tgctggcatg cgcaggcccc
     7561 gggcgactcg cgtctgggac agatcgatga ccgtgcggaa gggtcgctcg agagtgaaga
     7621 tccgcttcgt cgcgagcccg gtgacatcga gccgtaattg cgcagaatga gcggcgccgg
     7681 acaggtggac gccatgcaac tcggcgcccg cggcgagcgc agcgcccgcg gcacttagtg
     7741 cgatcagacc agcccccaac ttaaacatct tccttatcat ggagttcgac acgcgatctg
     7801 cggtgtaatc tacgtgccgg gcggcggctt ttcaaccaat gaagc
//
LOCUS       DASRHN010000292         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1917754, whole genome shotgun sequence.
ACCESSION   DASRHN010000292 DASRHN010000000
VERSION     DASRHN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917754"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2478)
                     /locus_tag="VFB37_11765"
     CDS             complement(<1..>2478)
                     /locus_tag="VFB37_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO23168.1"
                     /translation="QHTRAPEPESGHVPPRDDLERTLVSVWQAHLGDRPIGIRDNFFD
                     LGGHSMLAIRILFDIRARLQTQLDLVDIFDRPTIEELAGRVRQHSARGDSQAPVQALE
                     QIEPDPANRYEPFTLTDVQQAYWVGQTDAYALGNVGAHVYQETQFNGLDVERLQQSFR
                     KLIERHDMLRAVILPDGRQQVLEKVPEYDIPLFDLRSRSKEEQRVFIAGVRERMSHQV
                     FDLAKWPLFEVSACLIDDRHVRLHLSFDALIADAWSFEILLRDFSMLYMRPDVTLPAL
                     RLHFRDYVMATAGRPDAPLFLRAQAYWKDRIAALPPAPQLPMVKQLREIEAPKFGRRR
                     HIVPAEQWSRIKRKAAASGATPSALMLTAFAEALALWSRSQSFTLVLTVFNRPPLHPD
                     IDEIMGDFTSLVLVDFHPSTLPFGKRVLAVQKLLMERLEHRYFSGVRVLRELARGRTD
                     KATPLMPVVFTSTLTHERTEEAAFLKEQQVEAMGINQTPQVYLDHQLVEENGELQFNW
                     DSVDELFPDGLLDGMFEQYRAFVDSLADERTWDNVPDLLPHTAYDAYRKLNDTSVPRT
                     SGLAFDPILAHAARHPEAVAVITPARELSYGELLSISWKVAHALRKRGAKPGHIVGIL
                     LEKGWEQVAAVLGIQLAGAAYLPIDAQAPPERIEYLMADSGVQILVTNSALRQGLARE
                     AQYLLWDAPAWFETEPDTAMQLAQSPGDLAYLMYTSGSTGRPKGVMIEHRSIVNRMAD
                     VRQRFAITSADRAIGLTALHHDLSVFDIFGVLGAGGALVLPSAGRQHDPLHWMELLEA
                     ARVTLWNSVPSFLAMLTEFHANHSGVLL"
BASE COUNT          484 a          802 c          766 g          426 t
ORIGIN      
        1 gagcagcacg ccggaatggt tggcatgaaa ctccgtgagc atcgccagga acgaaggcac
       61 cgagttccac agggtcactc gcgcagcctc cagcaattcc atccaatgca acggatcgtg
      121 ctggcggccg gctgagggca gtaccaacgc cccgccggct ccgaggactc cgaagatgtc
      181 gaacaccgaa aggtcgtgat gcagcgccgt caggccgata gcgcgatctg ctgaggtaat
      241 cgcgaagcgc tggcggacgt cagccatgcg attgacgatg ctgcgatgct cgatcatcac
      301 ccccttcgga cgacccgtcg aaccggaggt atacatcaga tacgcgaggt cgcccggtga
      361 ctgagccaac tgcatcgcgg tatccggttc agtctcgaac caggccggcg catcccagag
      421 cagatactgc gcctcgcgcg cgagcccctg gcgcagcgca ctgttcgtga cgaggatctg
      481 taccccggaa tccgccatga ggtactcgat gcgctccggg ggagcctgcg catctatcgg
      541 caggtacgcc gccccagcga gctggatgcc caacaccgcg gcaacctgtt cccaaccctt
      601 ctcgagcagg atgccaacga tgtgccccgg tttggcgccc ctcttccgca gtgcgtgtgc
      661 gactttccac gagatggaga gcagctcgcc gtaagagagc tcgcgtgccg gggtaatcac
      721 cgcgacggcc tcgggatgtc gcgcggcatg cgcgagaatc ggatcgaacg ccagccctga
      781 tgtccgcggc acagaggtgt cgttcaattt ccgatatgca tcgtaagccg tgtgcggcag
      841 caggtctgga acgttgtccc aggtacgctc atcggccagc gagtccacga acgcgcgata
      901 ttgctcgaac attccatcca gcagcccgtc cggaaacaac tcatcgacgc tgtcccagtt
      961 gaattgcagc tcgccgttct cctccacgag ctggtggtcc aggtagacct ggggcgtctg
     1021 gttgatgccc attgcctcca cctgctgttc cttgagaaat gccgcctcct ccgtgcgctc
     1081 atgagtcaag gtgctggtga acacaaccgg catcaacgga gtggctttgt cggtgcgccc
     1141 tcgggccagt tcgcgcagca cgcgcacgcc gctgaagtag cgatgctcca ggcgctccat
     1201 caacagcttc tgcaccgcca gcacgcgctt accgaacggt agcgtcgagg ggtggaaatc
     1261 aaccagcacc agggaagtga agtcacccat gatctcgtcg atatccgggt gcagaggcgg
     1321 ccggttgaag acggtgagca ccagagtgaa gctctggctc cggctccaaa gcgccagcgc
     1381 ctccgcaaac gccgtgagca tcaaggccga cggcgtcgcg ccggaggccg ccgcctttct
     1441 cttgatccgg ctccactgct ccgccggaac gatgtgccgc cggcgcccga atttcggcgc
     1501 ctcgatctcg cgcagctgct tcaccatcgg cagttggggc gcgggcggca gagccgcgat
     1561 gcgatccttc caataagcct gcgcccgcag gaacagcgga gcgtcgggtc gccctgcggt
     1621 tgccatcacg tagtcacgga aatgcaagcg caaggcaggc agcgtcacat ccggccgcat
     1681 gtacagcatg gaaaagtcgc gcagcaggat ttcgaagctc caggcatcgg caatgagggc
     1741 atcgaaactg aggtgcaggc gcacatggcg gtcgtcgatg agacaggcgc tcacctcgaa
     1801 cagcggccac ttcgcaagat cgaacacctg gtgcgacatg cgttcacgca cacccgcgat
     1861 gaacacccgc tgttcctctt tggagcggct gcgcaggtcg aacaagggaa tgtcgtactc
     1921 aggcactttc tcgagcacct gttgccgccc gtccggcagg atcacggcac gcagcatgtc
     1981 atggcgctct atcaacttgc ggaaggactg ctgcaaccgt tcgacatcca ggccgttgaa
     2041 ctgggtctcc tggtaaacgt gagcacccac gttaccgagg gcgtaggcgt cggtctgacc
     2101 gacccaatac gcctgctgca cgtcagtcaa cgtgaacggc tcgtagcgat ttgccggatc
     2161 cggctcgatc tgctcgagcg cctgcacggg cgcctgagaa tcccctcggg ccgaatgctg
     2221 gcgcacccgg cctgccagct cctcgatggt cggccggtcg aagatgtcga ccagatcgag
     2281 ttgcgtctgc agccgggcgc ggatgtcgaa caggatccgg atcgcgagca tggagtgccc
     2341 gccgagatca aagaagttgt cccggatacc gatcggccga tcgcccagat gcgcctgcca
     2401 caccgagacg agggtcctct cgagatcgtc ccggggcgga acatgacccg attccggttc
     2461 cggagcgcgt gtgtgctg
//
LOCUS       DASRHN010000293        10238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1087826, whole genome shotgun sequence.
ACCESSION   DASRHN010000293 DASRHN010000000
VERSION     DASRHN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087826"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VFB37_11770"
     CDS             <1..1395
                     /locus_tag="VFB37_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HZO23169.1"
                     /translation="NTMTTMHALIMIFGAIMPAWTGLANWMIPMQVGAPDMALPRLNN
                     LSFWILPFAFVLLLGTLFVPGGGPAGGWTMYPPLMLQTGAAFPMVIFAIHLMGASSIL
                     GAINVIVTILNMRAPGMTLLRMPLFVWTWLITAYLLIAVMPVLAGAVTMLLTDHFFGT
                     TFFTAAGGGDPVMFQHIFWFFGHPEVYILILPAFGIVSEIIPTFSRKPLFGYETMVYA
                     VASIAFLSFIVWAHHMFTVGMPLAAQLFFMLSTMLIAVPTGVKVFNWTATMWRGSLSF
                     EPPMLFALAFLFLFSIGGFSGLMLAMVPADYQYQDTYFVVAHFHYVLVPGTIFGIMAG
                     VYYWLPKWVGKMYDLKLATWHFWLSTIFVNVLFFPQHFLGLAGMPRRIPDYAVQFTDW
                     NMVSSIGGFCFGLSQFLFPYIIWKCVRSGVPLTDNKAWEGAHGLEWELPSPAPYHSWQ
                     TPPSDAVIAKGAAH"
     gene            1407..1535
                     /locus_tag="VFB37_11775"
     CDS             1407..1535
                     /locus_tag="VFB37_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23170.1"
                     /translation="MAVNDEADRRRRVRRTTLVFALIAAAFYVGFIAMSVIRATGS"
     gene            1532..2074
                     /locus_tag="VFB37_11780"
     CDS             1532..2074
                     /locus_tag="VFB37_11780"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZO23171.1"
                     /translation="MSGTNRPHLNLTLKLLAIALGSFGFGFALVPLYSVLCSVTGYGD
                     QTALLRRVKAIEKPDPSRTITIEFLSNVASAGEWDFRPVVRTIDVHPGKLYSAEFFAH
                     NLTGRNTVAQAVPNIAPSKVAAYFHKTECFCFSPQHFNTDEQRNMPVRFIVDPAIPSY
                     VDRITLAYTFYDESSRVSSR"
     gene            2098..2982
                     /locus_tag="VFB37_11785"
     CDS             2098..2982
                     /locus_tag="VFB37_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZO23172.1"
                     /translation="MANAHAPGTGYYIPHGSPWPIRASVALFAIMLGFAAYLNDWAGG
                     WAFIPGAVLLAYMFFGWFSTVIDENQRGIFNGQVDHSFRMGMMWFIFSEVMFFAAFFG
                     ALFYARVLAVPWLAGQGTKVFTNLILWPSFDGTWPTNGPGHIGGHADSTFSVIPAFSL
                     PALNTAILLTSGLTVTIAHHALRAGRRSRLALFLALTFLLGFSFVALQAHEYGEAYRD
                     LGLRLSTGIYGSTFFMLTGFHGLHVTIGAIMLTVIWLRVLRGHFTPERHFAFEAVAWY
                     WHFVDVVWLGLFIFVYWL"
     gene            complement(2970..3206)
                     /locus_tag="VFB37_11790"
     CDS             complement(2970..3206)
                     /locus_tag="VFB37_11790"
                     /inference="COORDINATES: protein motif:HMM:NF022587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HZO23173.1"
                     /translation="MQNLIRLLILGVLVAIVASLGSALFHLSRGGGTDSRRLARALTV
                     RIALSLALFILLMIAWYLGLISPHGLATPAPHSQ"
     gene            3245..3985
                     /locus_tag="VFB37_11795"
     CDS             3245..3985
                     /locus_tag="VFB37_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZO23174.1"
                     /translation="MSIRIPIGSRLFAPSWKFTLLSVVLCAAFIALGRWQWHRGQLRQ
                     AEWDRFAAGTDRVLPLGPRGISEVTKYQRISVVGRFDGEHQFLLDNRVQHGRAGLEVL
                     TPLQRPNGRVLLVDRGWVPFAGFRDHLPDVGLRAPETLTVTGRVADLPVPGLPGGRAP
                     PTAQAPWPKLTTFPSMTQLSSTLGTALEPRILLLDPRDPYGYVRDWHPPGIEPVRNWS
                     YALQWWCFALVMVAIWVRLSVRPLVGGP"
     gene            3982..4584
                     /locus_tag="VFB37_11800"
     CDS             3982..4584
                     /locus_tag="VFB37_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23175.1"
                     /translation="MSTSANELRRRNLRTLALLAALFFVPLAIAFWMYYGTAWRPAAH
                     VNHGELITPPVALPAAALPSAISGAPVSPQLFRRKWSLVYVGDGSCNVACHHALYVMR
                     QVRLALNNQMTRVERVFLVTGGGCDREFLLREHAGLEVLDATGDEGRKLLARFPQQGR
                     DASLFVVDPLGNLMMRYDLHRDPKGLLEDLKKLLSLSQVG"
     gene            4591..5670
                     /locus_tag="VFB37_11805"
     CDS             4591..5670
                     /locus_tag="VFB37_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZO23176.1"
                     /translation="MHFSRHPAVLSDLPALRLSLWVRRLALAGVVLCFGVVVLGGYVR
                     LTAAGLGCPDWPGCYGHVTPLGAEESASAQAAFPARPLDVGKAWREMIHRYAASTLGL
                     IIVVIAATAIVTRRTGLIRVPYAIGLLLLVVFQGVLGMLTVTWRLKPLIVTGHLLFGL
                     TTLSLLWWLWLSLPAESPGPANGLAVQGPNSAVMRTNWRLALIGLVGLGCQIALGGWT
                     SSNYAAVACPDFPTCQNAWWPHADFRDAFVLWRGLAINFEGGVLDQPARVAIHLTHRL
                     GALMTTVLLSCAVAATLLRAAPRGAHRAAMAVLAALALQLAIGICMVLRGFPLWLATA
                     HTGGAALLLLSVLALLRNLLPAPPP"
     gene            5667..6590
                     /gene="cyoE"
                     /locus_tag="VFB37_11810"
     CDS             5667..6590
                     /gene="cyoE"
                     /locus_tag="VFB37_11810"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZO23177.1"
                     /translation="MSTRPDSAPRSSVVPRAPALWRRYLELTKPRVVALICFTAIVGT
                     LLASPGWPPWPALVWGNLGIALASACAATLNHVLDQRIDEQMTRTRTRPLPTGTLTQS
                     QALVFAAVLGVAAMAILAFLVNLLTASLTFVSLIGYAVIYTVWLKRATSQNIVIGGAA
                     GAAPPVLGWAAVTNTIDPNALLLFLIIFVWTPPHFWALAIARRDDYARAGIPMLPVTH
                     GIEFTRLHVLLYTVLLVCVTLLPFVTHMSGLLYLAVAVVLNVCFLYYALALKITARQE
                     LPMRVFKFSVTYLMWLFAALLADHYLQLIQG"
     gene            complement(6598..7032)
                     /locus_tag="VFB37_11815"
     CDS             complement(6598..7032)
                     /locus_tag="VFB37_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23178.1"
                     /translation="MMHTFLFEPGVWTSQGTFWRADGEPLEAQGRTEIAHHPECWLLA
                     GTLKVLGSPPVEFVNAYSISPPGRDGTSMKWTSENATLGKLSGTFNVVGSSILCVYTC
                     TASGYHGSEHLGQIDARRYQAAGVLLLNERRLSSWQVTLTRV"
     gene            7101..7886
                     /locus_tag="VFB37_11820"
     CDS             7101..7886
                     /locus_tag="VFB37_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HZO23179.1"
                     /translation="MERIWAANSFRNFHYLIACAQTGEALAIDPLDWRRCLEAARQKG
                     WEITQVLNTHEHLDHTGGNAGMVGATGARVLAHAGAASRIGGVDRALHKGDVIRVGTS
                     VELECLDTPGHTLSHVCLLAHTGQPALFCGDTLFNAGAGNCHNGGDPGRLYETFAAQL
                     ARLPDDTRVFPGHEYLARNLEFTLDREPGNAAAASLVEAARRHDPAAARVTTLGEEKE
                     INTFFRLRSPAVIARLREAFPEIGPEPDARTVFLKLRELRNSW"
     gene            complement(8016..8627)
                     /locus_tag="VFB37_11825"
     CDS             complement(8016..8627)
                     /locus_tag="VFB37_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZO23180.1"
                     /translation="MLTLYDYLDSGNGYKARLLLSQLGTPYRWVELDILKNETRTPQF
                     LAKNPNGRIPALELEDGTCLAESDAILWYLAEGTACLPRDRLGRAQVLQWMFFEQYSH
                     EPYVATPRYIVKHLPADSARRAELPERLTRGRAALAVMESHLREHLFFVGERYTIADI
                     ALYAYTHVAHEAGLEMAAYPAVNAWLARVASQPGYVPITHRPQ"
     gene            8675..9727
                     /locus_tag="VFB37_11830"
     CDS             8675..9727
                     /locus_tag="VFB37_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZO23181.1"
                     /translation="MYSSFYRRTFVIATILILGWALLLILRPLWTTIGWAAVLAFLLH
                     PVHERLSAKLKGRHSLAAGILTGLTPFCVMAPLAFLGVAFAKQVANILSAMSGSSLSY
                     SSVLQRLDSLPVVGGVVHWVRETAPVSAAQVKGWVLDGAQTVLKSAASVGGSVALGIM
                     GSLVGFFMMLFLLFFLLRDGRAMLTRLTGFIPMEPRPRGQLLDYLGHVTRAVVFGSAA
                     TAIIQGVFVGIAFAIIGLPSPLVFGVLAAFAAFLPAGAAIVLVPAVLYLVFVERWGAA
                     LFMVAWSAGVAVIDNVVRPFLTSRQAEVSTLAVFVGAIGGASAFGILGLVIGPVLLGF
                     IAALLQFAEKGVVTSE"
     gene            9847..>10238
                     /locus_tag="VFB37_11835"
     CDS             9847..>10238
                     /locus_tag="VFB37_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZO23182.1"
                     /translation="MDLSPILNPLNDAQRQAVTSPLGPILVLAGAGSGKTRVLTHRIV
                     WLIQAENASPHGVLAVTFTNKAAGEMRGRVEHLLGMPGGGLWIGTFHGIAHRLLRLHW
                     REAGLVQGFQILDSEDQQRLIRKLIRTM"
BASE COUNT         1538 a         3392 c         3230 g         2078 t
ORIGIN      
        1 aacaccatga ccaccatgca cgcgctgatc atgattttcg gcgcgatcat gccggcctgg
       61 accggcctcg ccaactggat gatcccgatg caggtcggcg cgcccgacat ggcgctgcca
      121 cggctcaaca atctgagctt ctggatcctg ccgttcgcct tcgtgttgct gctcggcacg
      181 ctgttcgtgc ccggtggtgg ccctgcgggc ggctggacga tgtatccgcc actcatgctg
      241 cagacaggag cggccttccc gatggtgatc ttcgccattc acctgatggg cgcctcgtcg
      301 atcctgggtg cgatcaacgt catcgtgacg attctcaaca tgcgcgcgcc cggaatgact
      361 ctgctgcgca tgccgctgtt cgtgtggacc tggctgatca ctgcctacct gctgatcgcc
      421 gtcatgccgg tgctggcagg tgccgtgacc atgctcctca cggatcattt cttcggcacc
      481 accttcttca ccgccgccgg cggcggtgac ccggtgatgt tccagcacat cttctggttc
      541 ttcggacacc ccgaggtgta catcctgatc ctgcccgcgt tcggcatcgt ctccgagatc
      601 attccgacgt tctcgcgcaa gccgctgttc ggctacgaga cgatggtgta tgcggtggcc
      661 tccatcgctt tcctgtcgtt catcgtctgg gcacaccaca tgttcacggt cggcatgccg
      721 ctggccgccc agctcttctt catgctctcc accatgctca tcgcagtgcc gaccggtgtg
      781 aaggtcttca actggacggc gacgatgtgg cgaggctcgc tctccttcga gccgccgatg
      841 ctgttcgcac tggctttcct gttcctgttc tcgatcggcg gcttctcggg gctgatgctt
      901 gcgatggtgc cggccgatta tcagtaccag gacacgtact tcgtcgtggc gcacttccac
      961 tacgtgctcg tgcccggcac gatcttcgga atcatggcgg gcgtgtacta ctggctgccg
     1021 aaatgggtcg gcaagatgta cgacctcaag ctcgccacct ggcatttctg gctgtcgacc
     1081 atcttcgtga acgtgctgtt cttcccgcag cacttcctgg ggcttgccgg catgccgcgc
     1141 cgcattcccg actacgccgt gcagttcacc gactggaaca tggtttcctc catcggagga
     1201 ttctgtttcg gtctgtcgca gttcctcttc ccctacatca tctggaagtg cgtccgctcg
     1261 ggcgtccccc tgaccgacaa caaggcctgg gagggagccc acgggctgga gtgggagctg
     1321 ccgtcgccgg cgccctatca ttcctggcag accccgccct ccgatgccgt catcgccaag
     1381 ggcgcagctc attgaggaga ggcaacttgg ctgtgaatga cgaagcagac cggcgccggc
     1441 gcgtgcgacg caccacgctg gtgttcgcgc tgattgccgc ggcgttctac gtgggcttca
     1501 tcgccatgtc agtcatccgc gctacaggct catgagcggc acgaatcgac cccatctcaa
     1561 cctgactctg aagctgctgg ccattgcgct cggcagcttc gggttcggct ttgcgctggt
     1621 cccgctctat agcgtgctgt gcagcgtcac gggctatggc gatcagacgg ctttgctgcg
     1681 gcgggtgaag gccatcgaga agcccgatcc gagccgcacg atcaccatcg agttcttgag
     1741 caatgtggcc agtgccggcg agtgggattt ccgtccggtg gtgcgaacca tcgatgtgca
     1801 ccccgggaag ctgtactcgg ccgagttctt cgcgcacaac ctgacaggac gcaacacggt
     1861 ggcgcaggcg gtcccgaaca tcgcaccctc caaggtcgcc gcctacttcc acaagaccga
     1921 atgcttctgc ttttcgccgc agcatttcaa cacggatgag cagcgcaaca tgccggtgcg
     1981 cttcatcgtc gatccagcaa taccctcgta cgtcgaccgg attacgctcg cctacacgtt
     2041 ctatgacgag tcatcgcgag tctcgtctcg ctagcagtac taaggaatac ggaagaaatg
     2101 gctaacgcac acgcccccgg taccggctac tacatacccc atggcagtcc gtggccgatc
     2161 cgggcttcgg tcgcattgtt cgcgatcatg ctcggcttcg ccgcgtacct gaacgactgg
     2221 gcgggaggct gggccttcat tcccggggcg gtgctgctcg cctacatgtt tttcggctgg
     2281 ttcagcacgg tcatcgatga gaatcagcgc ggcatcttca acggccaggt ggatcattcc
     2341 ttccgcatgg gaatgatgtg gttcatcttt tccgaagtca tgttctttgc ggcgttcttc
     2401 ggcgcgctct tctatgcgcg cgtactcgcg gtgccgtggc tggcggggca aggcaccaag
     2461 gtgttcacca acctgatcct gtggccgagc ttcgacggca cctggccgac gaatggcccg
     2521 gggcacatcg gcggccacgc ggactccacc ttcagtgtca tcccggcgtt cagcctgccg
     2581 gcgctcaata cagccatcct cctcaccagc ggattgaccg tgacgatcgc gcaccatgcc
     2641 ctgcgcgccg gacgccgctc caggctggcc ctgttcctgg ccctgacgtt cctgctcggc
     2701 ttctccttcg tggcgctgca agcccatgag tatggcgagg cctaccgcga cctcggcttg
     2761 cgcctgagca cgggcatcta cgggtcaacg ttcttcatgc tcaccgggtt tcacggcctg
     2821 cacgtcacga tcggcgcgat catgctcacg gtcatctggc tgcgcgtgct gcgcgggcac
     2881 ttcacgccgg agcgtcactt cgccttcgag gccgtggcct ggtactggca cttcgtcgat
     2941 gtggtctggc ttggcctctt catcttcgtc tactggctgt gaggggcagg tgtcgccagg
     3001 ccgtgaggtg agatgagacc gagataccag gcgatcatca gaagaatgaa gagcgcgagc
     3061 gatagtgcga tccgcaccgt cagcgcgcgg gcaagccggc gtgagtcggt gccaccgcct
     3121 ctcgagaggt ggaacagggc cgagcccagg ctcgcgacga tggccacgag cacaccgagg
     3181 atgagaagtc tgatcaggtt ttgcatgtgc gcggcgccca gcttataggc tgtcggcggc
     3241 ttgcgtgtcc attcgtattc ccatcggtag ccgcctcttt gcgccgtcct ggaaattcac
     3301 gctgctgagc gtggtcctgt gcgccgcatt catcgcgctc ggacgctggc aatggcatcg
     3361 aggacagctc cgccaggcag agtgggaccg gttcgcggcc ggcacggatc gtgtgctgcc
     3421 cctggggccg cgaggaatca gcgaagtcac caagtaccag cgcatcagcg tggtcggccg
     3481 gttcgacggc gagcatcagt tcctactgga caatcgcgtc cagcacgggc gcgcgggcct
     3541 tgaggtgctc acaccgctgc agcgtccgaa cggccgtgtc ctgctggttg accgaggctg
     3601 ggtacccttc gccggattcc gcgatcacct gcccgatgtc ggcctgcgtg ctcccgagac
     3661 gctcacggta acgggtcggg tcgccgacct gccggtgccc ggccttccgg gaggtcgcgc
     3721 gccgcccaca gcccaggcac cgtggccaaa gctcacgacc tttcccagca tgactcagct
     3781 cagcagtaca ttgggcaccg ctctcgagcc gcgtatcctg ctgctcgatc cgcgggatcc
     3841 gtacggatat gttcgcgatt ggcatccgcc cggaatcgag ccggtgcgca actggtcgta
     3901 cgcactgcag tggtggtgct ttgcgctcgt catggtcgcc atctgggtcc ggctcagcgt
     3961 tcgtcccctg gtcggggggc catgagcact tctgcgaacg agcttcgccg gcggaacttg
     4021 cgcacgctcg cgctgctcgc cgcactgttc ttcgtgccgc tcgccattgc gttctggatg
     4081 tactacggga ctgcctggcg ccccgccgca catgtcaatc atggcgagct gatcacaccc
     4141 cccgtcgcct tgccggccgc agcgctaccc tcggccatca gcggtgcgcc agtgtcgccg
     4201 cagctcttcc ggcggaaatg gtcgctggtg tacgtgggcg acggcagttg caacgtagcc
     4261 tgccaccacg cactttacgt catgcgtcag gtgcggctgg cgctgaacaa ccagatgacg
     4321 cgggtcgagc gtgtttttct ggtgaccggc ggcggctgtg accgcgaatt cctgttgcgc
     4381 gagcacgccg ggctcgaggt gctcgatgca acgggcgatg agggcaggaa actgctcgcc
     4441 cgcttccccc agcagggccg cgacgcgtcg ctcttcgtgg tggaccctct gggtaacctc
     4501 atgatgcgtt acgacctgca ccgcgatccg aagggtctgc tcgaagatct gaagaagctt
     4561 ctttccctgt cgcaggtcgg gtgagggccg gtgcacttct ctcgccatcc tgctgtcctc
     4621 agcgacctgc ccgccctgcg gctcagtctg tgggtgcgcc gcctggcgct cgccggtgtg
     4681 gtgctgtgct ttggggtggt ggtgctgggc ggatacgtac gattgaccgc cgccgggctt
     4741 ggctgtccgg actggccggg gtgttacggg catgtcacgc cgctgggtgc cgaggagagc
     4801 gcttcggcgc aggccgcctt tcctgcaagg ccgctggatg tcggcaaggc gtggcgggag
     4861 atgatccatc gctacgccgc ctcgactctg ggactgatca tcgtggtgat tgccgccacc
     4921 gcgatcgtga cgcgccgcac cgggctcatc cgcgttccgt atgcgatcgg cctgctgctg
     4981 ctggtcgtgt ttcagggtgt gctggggatg ctcacggtga cctggcgact gaagcctctg
     5041 atcgtgacgg ggcatctcct ttttgggctc accacgctca gtctgctgtg gtggctatgg
     5101 ctttccctgc cagccgagtc gcccggacct gccaacggcc tcgccgttca gggtcccaac
     5161 tctgccgtca tgcgcaccaa ctggcgcctc gccctgatcg ggctcgtcgg cctgggttgc
     5221 cagatcgccc tgggaggctg gaccagcagc aactacgctg ccgtcgcctg ccccgacttc
     5281 cccacttgtc agaacgcgtg gtggccgcat gcggactttc gggatgcgtt cgtactctgg
     5341 cgtggtctgg caatcaactt cgaaggcggc gtgctcgacc agcccgcgcg tgtcgccatc
     5401 cacctgaccc atcggttggg cgctctgatg acgacggtgc tgctgtcctg cgctgtggca
     5461 gccacgttgt tgcgcgcagc ccccaggggc gcccaccggg ccgcgatggc ggtactggcg
     5521 gcgctggccc tgcagcttgc gatcggaatc tgcatggtac tgcgaggctt tccgctctgg
     5581 ctcgcgaccg cgcacaccgg cggcgccgcg ctgctgctgc tgagcgtcct cgcgttactg
     5641 cgcaacctcc tgcctgcacc gccgccgtga gcaccagacc cgactccgcg ccacgctcta
     5701 gtgtcgttcc acgcgcgccg gcgctgtggc gtcgctacct cgagctgacc aagccccggg
     5761 tggtggcgct gatctgtttc acggccatcg tgggcacgct gctcgccagt cccggctggc
     5821 cgccctggcc tgcgctggtg tggggcaatc tcggcatcgc gctcgcttcc gcgtgtgcgg
     5881 ctaccctcaa tcacgtactc gatcaacgca tcgatgagca gatgacccgg acccgcaccc
     5941 ggccactgcc caccggtacc ctgacacagt cccaggctct ggtcttcgcc gccgtgctgg
     6001 gcgtagccgc catggcgatt ctcgcttttc tcgtcaacct gttgaccgcc tcactgactt
     6061 tcgtctcact gattggttat gcggtcatct acacggtgtg gctcaagcgc gccacctccc
     6121 agaacatcgt catcggcggc gcggcggggg cagcgcctcc ggtgctggga tgggctgccg
     6181 tcaccaacac catcgacccc aacgcgctac tgttgttcct gatcatcttc gtgtggacgc
     6241 caccgcattt ctgggcactc gcgatcgcgc gtcgcgatga ctatgcacgg gcgggaattc
     6301 cgatgctgcc ggtcacgcac gggatcgagt tcactcggct gcatgtcctg ctgtacaccg
     6361 tgctgctcgt gtgcgtgaca ctgctgccgt tcgtgactca catgagcggc ctgctgtatc
     6421 tggccgtcgc cgtggtgctg aacgtctgct tcctgtacta cgccctggcg ctgaagatca
     6481 cggcgcgcca ggagttgccc atgagggtat tcaagttctc ggtcacctat ctcatgtggc
     6541 tgttcgccgc gctcctggca gatcactatc tgcagctgat tcagggctga agagtagtca
     6601 gacgcgcgtc agggtcacct gccaggacga cagacggcgc tcgttgagca gcagtacacc
     6661 cgccgcctga tagcgccgcg catcgatctg acccaggtgc tccgaaccgt ggtagccgga
     6721 ggcggtgcac gtataaacac acaggatgct gctgcccacc acgttgaacg taccgctcag
     6781 cttccccaat gtggcgttct cggaggtcca cttcatgctg gtgccgtccc ggcccggcgg
     6841 gctgatggag tacgcgttga cgaattcgac aggcggagag ccgagcacct tgagcgtgcc
     6901 ggcgagcagc cagcattccg ggtggtgcgc gatctcggtc cggccctgcg cctccagcgg
     6961 ctcgccatcg gcgcgccaga aggtgccctg ggaggtccag acaccaggct cgaacagaaa
     7021 cgtgtgcatc atgcgccgct cctccttcag ccattatggc ccgcccggcc gtttaggggt
     7081 acgttaggct cactatgttg atggaacgga tctgggcggc aaacagcttc cgcaacttcc
     7141 actacctgat cgcctgtgcg cagacaggcg aggccctcgc catcgacccg ctggactggc
     7201 ggcgctgtct cgaggccgca cgccaaaagg gctgggagat cacgcaggtc ctgaataccc
     7261 acgagcatct ggatcatacg ggcggcaacg ccggaatggt gggcgccacg ggcgcccggg
     7321 tcctggccca tgcgggcgcc gcgagccgca tcggcggagt cgaccgtgcc ctgcacaaag
     7381 gcgatgtcat ccgggtggga acatcggtcg agctggagtg cctggacacc cccggccaca
     7441 cgctctctca cgtctgcctg ctggcccaca ccggacaacc cgctctgttc tgcggggaca
     7501 cattgttcaa tgcgggcgcg gggaattgtc acaacggcgg tgatcccggg cggctgtatg
     7561 agactttcgc ggcgcagctt gcgcggttgc ccgatgacac gcgtgtattt cccggtcacg
     7621 agtacctggc gcgcaatctg gagttcaccc tcgatcgcga gcccggcaat gccgcagcgg
     7681 cctcgctcgt cgaggccgcc cgccggcacg atcccgcggc cgcgagagtc actacgctgg
     7741 gtgaggagaa ggagatcaac accttcttcc gcctgcgcag cccggcggtc atcgcacgcc
     7801 ttcgagaggc cttcccggaa atcgggcccg agcctgatgc gcgtacggtg ttcctgaagt
     7861 tacgcgagct tcgcaactcc tggtagccgc accttgcctg gatcgccacc aggcacacgg
     7921 aggtgcccgc cgccacaggc ggcgagcgac gcgccgaaat cacgcttttg gcgcgttgcg
     7981 cgccagggtc ggcgcgattt ccaagcaatt aaaacttact gcgggcggtg cgtgatcggc
     8041 acgtatcccg gctggcttgc cacgcgcgcc agccatgcat tgactgcagg atacgcggcc
     8101 atctccaggc cggcttcatg cgctacatgc gtataggcgt aaagcgcgat gtcggcaatc
     8161 gtgtagcgct cgccgacgaa gaagagatgc tcgcggaggt gagactccat gaccgccagg
     8221 gctgcccggc cccgagtcag gcgctcaggg agctccgccc gccgcgccga gtcggcaggg
     8281 agatgcttca cgatgtagcg tggagttgcg acatacggct cgtggctata ctgctcgaag
     8341 aacatccact gcagcacctg agcgcgccct aaccgatctc gcggcaggca cgcggtgccc
     8401 tcggcgaggt accagaggat tgcatcggac tccgccagac aggtcccgtc ctccagctcg
     8461 agtgccggaa tccggccgtt gggatttttc gcaagaaact gcggcgtgcg ggtctcgttc
     8521 ttcaggatgt cgagctcgac ccatcggtag ggtgtgccga gctgcgacag cagcaggcgg
     8581 gccttgtagc cgttgccgga gtccagatag tcatacagcg tgagcatggc gcgctcccct
     8641 cgcccgaagg cccagacata gtatcctgag cgctgtgtac tcgtcgttct accgccgcac
     8701 cttcgtcatc gcgacgatcc tgatcctcgg ctgggcgctg ctgttgatcc tgcgcccgct
     8761 gtggaccacg atcggatggg ccgcggtcct cgctttcctc ctccatccgg tgcacgagcg
     8821 gctcagcgca aaactcaagg gacgtcactc acttgcagcg gggattctca cgggcttgac
     8881 gccgttctgc gtcatggcgc cgttggcctt cctgggcgtc gcgtttgcga aacaggtcgc
     8941 caacatcctg tctgccatgt cgggcagctc gttgagctat agctcggtgc tgcagcggct
     9001 cgattcgctg cctgttgtcg ggggggtcgt ccactgggtg cgggagaccg cgccggtgag
     9061 tgccgcccag gtgaagggct gggtcctcga tggagctcag acggtgttga aatcggcggc
     9121 ctcggtgggc ggcagcgtgg cgctggggat catgggctcg ctggtcggtt tcttcatgat
     9181 gctgttcctg ctgttcttcc tgctacgcga tggacgcgca atgctcactc gactcaccgg
     9241 gttcattccc atggaaccgc gcccccgcgg acagcttctc gactacctgg gccacgtcac
     9301 gcgcgcagtc gtgtttggat cggcggcgac cgccatcatc cagggtgtgt ttgtcggcat
     9361 cgctttcgcg atcatcggcc tgccttcacc gctggtcttc ggagtgcttg ccgcattcgc
     9421 cgcattcctg cccgccggcg cagccatcgt gctggtgccc gccgtgctgt atctggtttt
     9481 cgtggagcgc tggggcgcgg ctctcttcat ggtggcgtgg agtgccgggg tggctgtcat
     9541 cgacaacgtc gtgcggcctt tcctcacttc gcgccaggcc gaggtgtcca ccctggcagt
     9601 gttcgtgggt gccatcggcg gtgcatccgc tttcggaatc ctggggctgg tcatcggccc
     9661 ggtgctgctg gggttcattg ctgccctgct gcagttcgcc gagaaaggcg tggtcaccag
     9721 cgagtaggcg ccttctctta tcgaggacgc ctgagttggg cgcgtgggtc aagccccctt
     9781 gcgccggagc tctcttccag ggggcgaacc cacgcgcctg gctcaggcgc ctataatgcg
     9841 ccccccatgg atttgtcccc gatcctgaat cccctcaacg acgcgcagcg ccaggccgtc
     9901 acctccccgc tcggacccat cctcgtgctg gccggcgcgg gcagtggcaa gacgcgcgtg
     9961 ctgacgcacc gcatcgtctg gctcatccag gccgaaaacg cctcgccgca cggcgtgctc
    10021 gccgtaacgt tcacgaacaa ggcggcgggg gagatgcgcg gccgcgtcga gcacctcctg
    10081 gggatgccgg gcggcggact gtggatcggt accttccatg gcatcgcaca tcggctgttg
    10141 cggttgcact ggcgcgaagc ggggctggtg cagggcttcc agatcctcga ttccgaggac
    10201 cagcagcgcc tcatccgcaa gctcatccgc acgatgga
//
LOCUS       DASRHN010000294         4530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_100907, whole genome shotgun sequence.
ACCESSION   DASRHN010000294 DASRHN010000000
VERSION     DASRHN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100907"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..386)
                     /locus_tag="VFB37_11840"
     CDS             complement(3..386)
                     /locus_tag="VFB37_11840"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZO23183.1"
                     /translation="MGSNPIDRSTQQIGFAAEEAAALFLRAEGFTIVLRNFRRRLGEL
                     DIIARAQEVLIIAEVRTRSGLTHGGAAASVGLRKQRRVVRAAAQLLQRRKDLAQLRVR
                     FDVLVVHDACGPQPRIEWIQHAFSA"
     gene            complement(418..2307)
                     /locus_tag="VFB37_11845"
     CDS             complement(418..2307)
                     /locus_tag="VFB37_11845"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZO23184.1"
                     /translation="MLLVRMKEALGLAVLAALMLTLAGCPSLSPRAEAPPSVDTAEAL
                     ARRGDQGGAARMFEALAGQNSGADRTQYLFRAAHAYLAARRADDAARVLASIEPPLTP
                     QQTAERGMLDVEVALARGQGAQAWQRISALPEPKTAPEASRYLRLKEQAAFAAGRPTD
                     GVIAEVALERWLPSVQDLHQSRTALLGSLRDASEHGARIDPRSAPDATVRGWLELGPI
                     AAAAARNPNGAVADIDAWRSRYQNHPANELVRSELLGRETQPPAQIEAHIALLLPLSG
                     RQAAAGMNVRDGFLTAYYQTPVAQRAPIHIYDTSDAGIAETVARATQEDAQIIVGPLT
                     REEVVAAAGLTNRRPPILALNFLPSDRPAPAAFYQFALSPEEEARQTARRILADGHRH
                     GVAIVPAGDWGSRVLNAFTQELQAGGGDLFASATIDTAVADFSDAITRVLRINDSEAR
                     HRRLESILGTKLQFEPRRRGDLEFIFAPAPANIERLLRPQLRFHFAGNVPTYATSDAF
                     EPDPRSNQELEGLMFPDMPWLLGGELPDAVRTVVHDAWPNGSGPRLGPLFAFGFDAYR
                     LGMALRANPEANTIQIDGLTGHLTMDADRRVHRELGWAQLHNGEIRLLPAPSAPAPAQ
                     PAPAT"
     gene            2374..3240
                     /gene="rsmI"
                     /locus_tag="VFB37_11850"
     CDS             2374..3240
                     /gene="rsmI"
                     /locus_tag="VFB37_11850"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZO23185.1"
                     /translation="MDKARGRLEVIATPIGNLNDLSPRARQALEQADLIAAEDTRHTL
                     GMLQALGISRPLVSVHAHNEPQRVPEILERLAGGEVVALVSDAGMPLLSDPGFELVSS
                     AARAGFEVRVVPGPSAVTAALAIAGLPTSRFCFEGFLPARSRERRTHLAGLAQEVRTL
                     VFFEAPHRIAATLADLAQEFGEGRFAVVAREMTKAHETIYRGTLAQLAQQAASETNFQ
                     RGELTVVVQGAADVGGGVDSKLLRRAVELLSRELPPGRAAAIAAQLTGATRSDAYALL
                     TRAREQPPGEEG"
     gene            3320..3742
                     /gene="rnpB"
                     /locus_tag="VFB37_11855"
     ncRNA           3320..3742
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFB37_11855"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4180..>4530
                     /gene="mraZ"
                     /locus_tag="VFB37_11860"
     CDS             4180..>4530
                     /gene="mraZ"
                     /locus_tag="VFB37_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZO23186.1"
                     /translation="MFRGATKVTLDDKGRMVMPTRYREQISESGQGHLVVTVDRDQCL
                     LIYPLPEWDRIERKLMSLPSLHAQARRLQRLMVGHATDVEIDGHGRVLLPPELREFAR
                     LERHGMLIGQGNRFE"
BASE COUNT          764 a         1455 c         1566 g          745 t
ORIGIN      
        1 attcaagcgc tgaacgcgtg ctggatccac tcgatgcgcg gctgcgggcc gcaggcatcg
       61 tgcacgacga gcacatcgaa gcggacgcgc agctgggcga gatccttacg ccgctgcagg
      121 agctgggctg cggctcgcac aacccgccgc tgcttgcgca atccaacact ggccgctgct
      181 cctccgtgtg tgagccctga tcgggtgcgg acttcggcga tgatcaaaac ctcctgcgcc
      241 cgcgcgatga tgtcgagctc gccgagccgg cggcggaaat tcctgagcac gatggtgaag
      301 ccctcggcgc gcagaaacag agcggccgcc tcttcggcgg cgaagccgat ctgctgcgtg
      361 ctgcggtcaa tggggttgga acccatctga cacctccttg tgtccgtagc tcggatctca
      421 ggtggccggt gcgggttgcg ctggagcagg tgctgagggc gccgggagca accggatctc
      481 gccgttgtga agctgcgccc atccgagctc gcgatgcacg cggcggtcgg catccatcgt
      541 cagatggccc gtcagaccgt cgatctggat ggtgttggct tcagggttgg cacgcaacgc
      601 catgcccaac ctgtaggcat cgaaaccaaa ggcgaacagc gggccgaggc gtgggccgct
      661 accgttgggc caggcatcgt gcacgacagt acgaacggcg tcgggcaact ctcctcccaa
      721 cagccaaggc atgtcaggga acatgagtcc ctcgagctcc tgattgctgc gcggatcggg
      781 ctcgaaggca tccgaggtgg cgtaggtggg cacattgccg gcgaagtgaa accgcagctg
      841 gggtcgcagc agccgctcga tgttggcagg cgccggggcg aagatgaatt ccagatcacc
      901 gcggcggcgc ggctcgaact gcagcttcgt gcccaggatc gactcgagtc ggcggtggcg
      961 cgcctcgctg tcgttgatgc gcagtacgcg tgtgatggcg tccgagaaat ctgccacggc
     1021 tgtgtcgatc gtggctgaag caaacaggtc gccgcccccc gcctgcagct cctgggtgaa
     1081 ggcgttgagc acccgtgagc cccagtcccc tgcgggcacg atggcgacgc cgtgccgatg
     1141 cccatccgcg aggatgcgac gggcagtctg ccgcgcctct tcctccggcg acagtgcgaa
     1201 ctggtagaaa gcggccggtg ccggccgatc ggagggtagg aagttgagcg ccagaatggg
     1261 cggccggcga ttggtcaggc cggccgcggc gacgacctct tcgcgagtga gcggcccgac
     1321 gatgatctgc gcatcctcct gggttgcgcg cgccaccgtc tccgcgattc ccgcatcgct
     1381 cgtgtcgtag atgtgaatcg gcgcgcgttg cgcgacgggc gtctggtagt acgcggtcag
     1441 aaagccgtcg cggacattca tccccgcagc ggcctgacgc ccgctgagtg gcagcaggag
     1501 cgcgatgtgc gcttcgatct gagccggtgg ctgggtctcg cgcccgagca gctcactgcg
     1561 caccagctcg ttcgcggggt gattttgata acgcgagcgc caggcatcga tgtcggccac
     1621 cgcgccattc gggttgcgcg cggccgctgc ggcaatcggt cccaactcga gccagccgcg
     1681 tacggttgca tcgggcgcac tgcgcggatc gatgcgcgcg ccgtgctcgc tcgcatcgcg
     1741 cagcgacccc agcagggccg tgcggctctg atgcaaatcc tggacggacg gcagccagcg
     1801 ttccagcgcg acctcggcga ttacgccatc ggtgggtcgt ccggcagcga aggcagcctg
     1861 ctctttcagg cgcagatagc gcgacgcttc aggcgccgtt ttcggctccg gaagtgcgct
     1921 gatccgctgc caggcctgcg cgccctgccc gcgcgcgagt gccacctcca catccaacat
     1981 gccccgctcc gcagtctgct gtggagtcag ggggggctcg atcgaggcca gcacccgcgc
     2041 tgcatcgtcg gcgcgccggg ccgccagata cgcgtgcgcc gcgcgaaaca aatactgcgt
     2101 ccggtccgct ccgctgttct gtcccgcgag cgcctcgaac atccgcgccg cgccaccctg
     2161 atccccccgg cgcgccagcg cctcggcggt atcgactgaa ggcggcgctt cggcgcgcgg
     2221 gctgaggctc gggcagccgg ccagagtgag catcagcgcg gcgagcacag cgaggccgag
     2281 cgcttctttc attcgcacga gcagcatcga ggatcacttc ggacatggcg tcgcggccgc
     2341 gggcgactat agtaaacgat ggaggcgact ggagtggaca aagcacgcgg ccggctggag
     2401 gtcattgcga ctccgatcgg caacctgaac gatctgagcc cgcgcgcacg ccaggcgctc
     2461 gagcaggccg atctgatcgc cgcggaggac acccggcaca cgctcggcat gttgcaagca
     2521 ctcggcatct cacggccgct cgtgtcggtt catgcccaca atgagccgca gcgagtgcct
     2581 gagattctcg agcggctcgc cggtggcgag gtcgtcgcac tcgtcagcga tgccggaatg
     2641 ccgctgctat cggatccggg tttcgagctg gtgagcagcg ccgcgcgtgc aggcttcgag
     2701 gtgcgagtcg tgccggggcc gtcggcggtc acggccgcgc ttgcgatagc cggactgccg
     2761 acgagccgct tctgcttcga aggctttctg cccgcgcgct ctcgcgagcg ccgtacgcat
     2821 ctcgccgggt tggcgcagga agtgcgcacg ctcgtgttct tcgaggcgcc gcatcggatc
     2881 gcggcaacgc tcgcggatct ggcgcaggag tttggtgagg ggcggttcgc ggtggttgcg
     2941 cgggagatga ccaaggcaca cgagaccatc tatcgcggca cactcgctca gcttgcgcaa
     3001 caggcggcga gcgaaaccaa cttccaacgc ggcgagttga cggtggtggt gcagggcgca
     3061 gctgatgttg gtggaggcgt cgattccaaa cttttgcgtc gcgcggtcga gttgctctcg
     3121 agggagttgc ctcctgggcg ggccgcagcc attgctgcac aattgacggg tgcaactcgc
     3181 agcgatgcgt acgcgttgtt gacacgcgca cgcgaacagc ctcccgggga ggagggctga
     3241 gggctgggtc ctggccagtg gcttgttgaa acgcccgccg atttgacttc tgcagccggg
     3301 cccttaaggt ggccgccgga gagccggccg gacagtcgct gctcctcccg ggccgtcaca
     3361 agcctggtgc gggggtggag gaaagtccgg gctccggcgg atcaggtgcc aggtaacccc
     3421 tggggggcgc gagcccacgg aaagtgcaac agaaagcaaa ccgcctaagt ccttccggag
     3481 ccgtgtaagc ggtctcggca gggccggcaa gggtgaaacg gtgcggtaag agcgcaccgc
     3541 ggtcctggta acagggctgg cacggtaaac cccacctgga gcaaggccaa atagggaagc
     3601 cgcagggccc cctcatcgca gggagcggct gcagcaccga tccgcgtgcg cttccgggta
     3661 ggccgcttga ggtccacggt gacgtggatc ccagaggaat gactgtcctc gacagaaccc
     3721 ggcttacagg ccggctctcc ttcacttaat ccggggcctc ggagcgaccc gccgggtgtg
     3781 cccgcgagag atctaggtat gcccggattg gcccgccagc agcgacccgg agcagcccgc
     3841 taaaggcccc ccagaacggg tcccccgcgc cccggaaaca ggtcgccgga ggcccgggaa
     3901 cgcccccgcc ggaggccccc caaacggtcc ctcagggcgc tctggaggcc ctacagccga
     3961 gtcttcgcta aaccactgta taaacttcgg cggacaagcc gctgattgct aagacgaatg
     4021 actgcaaaac actgtgcacg ggtcgtccgg cctcgtataa gtctttgtga ctgcgatgaa
     4081 tttcacacta tttcaattga catagtgcct ccctgattcc atagtggcgc ccggtgggag
     4141 aaagtggggg gaaatgggcg agtcctcgcc ttccgggtca tgtttcgcgg tgcaacaaag
     4201 gtcaccctgg acgacaaggg tcggatggtc atgcctaccc ggtaccggga gcagatcagc
     4261 gagtccggcc aggggcatct ggtcgtcacc gtggaccggg atcagtgcct cctcatttac
     4321 cccctccccg agtgggatcg tatcgaacgc aagctgatga gcctgcccag tctgcatgcg
     4381 caggcgaggc gtctccagag gctcatggtc ggccatgcga ccgatgtgga gatcgacggg
     4441 cacggccggg tgctcctgcc gcccgagctg cgtgagttcg caagactcga gcggcacggg
     4501 atgctgatcg gccaggggaa ccgtttcgag
//
LOCUS       DASRHN010000295         8515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1748310, whole genome shotgun sequence.
ACCESSION   DASRHN010000295 DASRHN010000000
VERSION     DASRHN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748310"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /gene="gspD"
                     /locus_tag="VFB37_11865"
     CDS             complement(<1..1770)
                     /gene="gspD"
                     /locus_tag="VFB37_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HZO23187.1"
                     /translation="MRCTLLALGVASLASAQQTGRITPNFKDADITQITEAVGAATGK
                     TFIIDPRVRAQVTMLSSTPMTPAAFYQAYLAILQVHGFIAVQNGDIVKVLPDANARQV
                     PSVDLPSHVSSTSDEIVTQVIDVKNVSAAQLVPILRPLIPQYGQLAAYPAGNILIISD
                     RANNVDRITRIVRRIDQVGDQDVEIIPLQNASSAEIVRVVNSLYQGQGAAEAGQQVKV
                     VADDRSNSVLVGGDQAQRLRIRALIAHLDTPLDSGGDTQVRYLHYADAEKIAPKLKEQ
                     ITGVAQAAAGTAPGAAASPQAQAEKSAMVWADPQNNALVITAPPKIMRAVMSIVDKLD
                     IRRPQVLVEAVIVEVNTDKTAQLGVNWAEFERGTGTVPAGAFVSPIGSSGSIVDLAND
                     VGGLVTGTATSATLPTGATFAVGRISSGGLNFAAMLSALRGDSDTNVIATPSTMTLDN
                     QEAEIKVAEEVPFVTGSFANTGTVSNGAVNPFQTIQREEVGTILKVTPNIAAEGNSVV
                     LKISIESSSVLPTSVSSVDITTSKRTITTNVLIEDGGIVVLGGLISDSNSKDSQQVPF
                     LGSIPIIGQFFKTRHTEATKNNLM"
     gene            complement(1853..2833)
                     /gene="gspC"
                     /locus_tag="VFB37_11870"
     CDS             complement(1853..2833)
                     /gene="gspC"
                     /locus_tag="VFB37_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HZO23188.1"
                     /translation="MNAASWLEGLPAPEKWRALLLSQGPRIATWVLALALSVQAAVIL
                     TNLAGAGRTPKLIGTAAPAPVMRRSVDVAAITNMHLFGTAPANATTAQDAANAPQTSM
                     PLVLTGIIAADDPHDGLAILGQNAGAAKVYAVGDNIPGGAKLHSVYVERVVIDRNGRL
                     ESLVLPRQVQGSAAPPSAAALQTESPVMDRMRKLIADDPGLMADIMRPQPVFAGGRQK
                     GYRVYPGRNREAFSRLGLHPGDLVTAINGTPLDDPARGQEIFRTLGSSSEAHITVMRS
                     GQQQDLTLNIAQVAQEAEALAGSQGASPDQGQPPVPPPPSEPPNQPDSSQ"
     gene            3061..3381
                     /gene="clpS"
                     /locus_tag="VFB37_11875"
     CDS             3061..3381
                     /gene="clpS"
                     /locus_tag="VFB37_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZO23189.1"
                     /translation="MPDPHPARPDTGIVVEESRPQVKQPPLYQVVLLNDDYTPMEFVV
                     DVLEKFFALNRPTATRIMLEVHTKGKGVCGVFTYEIAETKVAQVTTYARDHQHPLMCT
                     LEEA"
     gene            3494..5767
                     /gene="clpA"
                     /locus_tag="VFB37_11880"
     CDS             3494..5767
                     /gene="clpA"
                     /locus_tag="VFB37_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HZO23190.1"
                     /translation="MLSNELEYCLNDAFHQAREARHEYLTVEHLLLAILDTPKVREIL
                     RACGADLGKLKQELKDHVDQSTPRLEEGEEREVQPTLGFQRVLQRAVFHVQSSGKKEV
                     GVGNVLVAIFSEKQSHAVFLLNRQHVTRLDVVNYISHGLSKIAEEKTTTDKEDTQSDG
                     ERDPEGGSALEKYTTHLNRLAQDGRIDPLIGRKLEVERTIEILCRRRKNNPLYVGEAG
                     VGKTAIAEGLARLIVEGKVPEVLSDCTLYALDMGALIAGTKYRGDFEKRLKGVITELK
                     KQPGAILFIDEIHTVIGAGAASGGVMDASNLIKPVLTNGEIRCIGSTTYQEYRGIFEK
                     DHALARRFQKIDVVEPSVAETVEILMGLKPRFEEHHGITYANDALKAAAELAARHINE
                     RHLPDKAIDVVDEAGARQRLKPVAEREPLIEVRHIEDIVARMARIPPKSVSTSDREVL
                     RNLERNLKLVIFGQDKAIESLAASIKMARSGLGDQRKPVGSFLFAGPTGVGKTEVTRQ
                     LAICMGVEFLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKHPHCVLLLDE
                     VEKAHPDVFNLLLQVMDHGTLTDNNGRKADFRHVIIVMTTNAGAQEMSRPSIGFTQSD
                     NTSDGMEAIRRIFTPEFRNRLDAVIQFASLDPITIERVVDKLLVEVETQLEAKGVSIT
                     LDDAARRWVAEKGYDPKMGARPMARLIQEHIKRPLAEELLFGKLVGGGQVTVTVSEDG
                     SKLKLDSVPTQLPEVTA"
     gene            complement(5798..6016)
                     /gene="infA"
                     /locus_tag="VFB37_11885"
     CDS             complement(5798..6016)
                     /gene="infA"
                     /locus_tag="VFB37_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZO23191.1"
                     /translation="MSKEDAIQMEGEVVETLPNTTFRVKLKNGHVVTAHISGKMRKNY
                     IRILTGDAVTVEMTPYDLTKGRIIYRGR"
     gene            complement(6116..6802)
                     /gene="aat"
                     /locus_tag="VFB37_11890"
     CDS             complement(6116..6802)
                     /gene="aat"
                     /locus_tag="VFB37_11890"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZO23192.1"
                     /translation="MRSITWLSPQDAPDSFPAPEQALEDPPGLLAAGGDLSPARLLAA
                     YRRGIFPWYSPGQPVLWWSPDPRAVLFPEEFRCARSLAKTLRNGGFSLAVNRDFAAVI
                     DSCAAPRPHSPGTWITPEMRNAYLQLHRLEYAHSIEAYRQGVLVGGLYGVRLGSVFFG
                     ESMFSRERDASKVALAHLVSLCRDNGIAVIDCQLASRHLQSLGSRAIARSQFLSLLRK
                     HIGTQALRIS"
     gene            complement(6799..7959)
                     /locus_tag="VFB37_11895"
     CDS             complement(6799..7959)
                     /locus_tag="VFB37_11895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO23193.1"
                     /translation="MRTRVHKSIDELSPQQWNALVPDRNPFLRHEFLAALEHTGCIGP
                     GTGWNPCYLSLSDPSGLAAAVPAFAKGHSYGEFVFDFAWAQAYSRFGLRYYPKLTVAV
                     PFTPATGARLLVRPDLDSTATTRRLLTELENHTQGHALSSVHALFLDEPGRAACEHAG
                     WLTRRDCQFHWRNQGYASFDEYLDTFTAEKRKKARRERRRVAEAGIVFETRMGADLDE
                     RLLDCVYEFHRDTFLRHGHEPYLTRAFFSEVARTLGDALMVKLAVHRSRPVAAAIFFW
                     CPQALFGRYWGASAEYNSLHFEACYHQGVEFCIERGVGRFEPGTQGEHKVSRGFEPTL
                     TWSGHFIANRRFRTAIAEYLQQEGASVDAYAQEVRQHIPYRDKRSAGGGEIA"
     gene            complement(7928..>8515)
                     /locus_tag="VFB37_11900"
     CDS             complement(7928..>8515)
                     /locus_tag="VFB37_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO23194.1"
                     /translation="CATCDGFFFRGHKVAVAGGGNTAVEEALYLSHIAQHVTLVHRRE
                     KLRAEKILQDRLFREVESGKISVIWSHTVEEVLGDQHGVQGMRLRSTDGPGTREIDIT
                     GLFVAIGHSPNTAIFAGQLEMREGYIRTRSGLEGNATATTIPGVFAAGDVADHVYRQA
                     ITSAGAGCMAALDADRYLETLDVATDAHASTQKHR"
BASE COUNT         1512 a         2645 c         2863 g         1495 t
ORIGIN      
        1 catcaggttg ttcttcgtgg cctccgtgtg ccgggtcttg aagaactggc cgatgatggg
       61 aatgctgccg aggaagggca cctgctggct gtccttcgag ttcgaatccg agatgagccc
      121 gcccagcacc acgatgccgc catcctcgat gagcacgttg gtggtgatgg tacgcttgct
      181 ggtcgtgata tccaccgagg agaccgaggt cggcagcacg ctcgaactct cgatggagat
      241 cttcagcacc accgagttgc cttccgcggc aatgttggga gtgaccttga ggatcgtgcc
      301 cacttcctca cgctggatcg tctggaaggg gttgaccgcg ccgttgctga ccgtgccggt
      361 gttggcgaac gagccggtga cgaagggcac ttcctcggct accttgattt ccgcctcctg
      421 attgtcgagc gtcatcgtcg agggggttgc gatgacgttg gtatccgaat ccccgcgcaa
      481 cgcgctcagc atggcggcga agttgagacc gcccgaggag attcgcccga cagcgaacgt
      541 cgcgccggtg ggcaaggttg cggaggtggc cgtgccggtg acgaggcccc cgacatcgtt
      601 cgccagatcg acgatgctgc ccgagctgcc gatggggctg acgaatgcgc cggcgggaac
      661 cgtgcccgta ccgcgctcga actcggccca gttgacaccg agctgagcgg tcttgtcggt
      721 gttgacttcg acgatcacgg cttcgacgag cacctggggc cggcggatgt cgagcttgtc
      781 cacgatgctc atgaccgcgc gcatgatctt gggcggtgcg gtgatcacga gcgcgttgtt
      841 ctgcggatcg gcccacacca tcgcgctctt ctcggcctgc gcctgcgggg aggcggcggc
      901 accgggggcg gtgcccgcag cagcctgagc gaccccggta atctgctcct tcagcttcgg
      961 agcaatcttc tccgcatcgg cgtaatgcag gtagcgcact tgcgtgtcac cgccggaatc
     1021 gagcggcgta tccagatgcg cgatcaaggc gcggatgcgc aggcgctggg cctgatcgcc
     1081 tcccaccagc acgctgttgg agcggtcatc ggcgacgacc ttgacctgct ggccggcctc
     1141 ggccgcgcct tgtccctgat aaagagaatt caccacgcgc acgatctcgg cggaggaggc
     1201 attctgcagc gggatgatct ccacatcctg gtcgcccacc tgatcgatgc ggcgcacgat
     1261 gcgggtgatg cggtcaacgt tgttggcgcg gtccgagatg atgaggatgt tgccggccgg
     1321 gtaggcggcg agctggccgt actgaggaat gagcgggcgc aggatcggca cgagctgcgc
     1381 ggcactcacg ttcttgacat cgatcacctg ggtcacgatc tcatcggagg tcgagctgac
     1441 atgagatggc agatcgacgg agggcacctg gcgcgcgttg gcatcgggca ggactttcac
     1501 gatgtcgccg ttctgtacgg cgatgaatcc atgcacctgc aggatggcga gatacgcctg
     1561 atagaacgcg gcgggcgtca tcggcgtgga ggaaagcatc gtgacctggg cgcgcacgcg
     1621 tgggtcgatg atgaaggtct tgccggtggc ggccccgacc gcctcggtga tctgagtgat
     1681 atccgcgtcc ttgaagttgg gggtgatgcg cccggtctgc tgcgcggagg cgagcgaagc
     1741 cacccccagc gccaggagcg tgcaacgcat cagaaagcgc agccggcggc tttccgacct
     1801 ggacgaccga attccattca ctagacgttc ccccgaagcc ggcgtaccgg cactactgcg
     1861 atgagtcggg ctgattcggc ggctccgacg gtggcggagg caccggcggt tgcccctgat
     1921 cagggctcgc gccctgggag ccggccagcg cctcggcttc ctgcgcgacc tgcgcaatgt
     1981 tcaatgtgag atcctgctgc tgcccgcttc tcatgactgt gatgtgtgcc tcactggagg
     2041 atcccagtgt gcggaagatt tcctgtccgc gagccggatc atccagcggc gtgccattga
     2101 tcgccgtcac caggtccccg gggtgcagtc cgagacggct gaaggcctcc cggttgcgtc
     2161 ccgggtagac ccgatagccc ttctgccggc ccccggcgaa caccggttgg gggcgcatga
     2221 tgtcggccat gagcccgggg tcgtcggcga tcagtttgcg catgcggtcc atgaccggcg
     2281 attcggtctg cagagcggcc gccgagggtg gtgcggcgga cccctgcacc tgccggggca
     2341 gcacgaggga ctcgagccga ccgttacgat cgatcaccac gcgctcgacg tacaccgagt
     2401 gcagcttcgc gccgcccgga atgttgtctc cgaccgcata caccttggcc gccccggcat
     2461 tctggccgag gatggcgagg ccatcgtgtg gatcgtcagc ggcgatgatg ccggtgagca
     2521 ccaatggcat gctcgtctgc ggtgcattgg cggcatcctg agccgtagtc gcattcgccg
     2581 gcgccgtccc gaatagatgc atgtttgtaa tggcggccac gtccacgctt ctgcgcatca
     2641 ccggagccgg cgcggcagtg ccgatcagct tgggcgtgcg gccggcgccg gccaggttgg
     2701 tgaggatcac cgccgcctgc acgctgagcg cgagcgccag cacccaggtg gcaatacggg
     2761 gaccctgcga tagcagaagc gcgcgccact tctcgggcgc cggcaatcct tcgagccagg
     2821 atgcggcatt cattggccga tcgaatcctc gtgagccccc acgaaccgca gcttcaggca
     2881 ggacttgcat gccaagcgcg cccggctgcc gcgagcacag ccgtggtgcg gtccggtgcc
     2941 cgtgcagggg caatcatacg gggctgtcgc acatgctctc aagcaattgt ttctgctggt
     3001 taaagcgtgg ctcatcatca caggcttgtg ttgccgtttg gcgaccccta taaagacgca
     3061 atgcccgatc cgcatcctgc acgccccgat accggcatcg tcgtcgagga gtcgcgcccg
     3121 caggtcaaac agcctcccct ctatcaggtc gtcctgctca acgacgatta cactcccatg
     3181 gagtttgtgg tggatgtgct cgaaaagttc ttcgcgctca accggccgac tgcgacgcgc
     3241 atcatgctgg aggtccatac caagggtaag ggcgtctgtg gggtcttcac atacgagatc
     3301 gctgaaacta aggttgcaca ggtgacgacc tacgcgcgag accatcagca cccgttgatg
     3361 tgtacattgg aagaggcgta aaggagtgga agagagcagc atggatgaat ggagtggaat
     3421 gactgcctgt gcggtgctgg tatcccgttc agcggatcca gcggatggaa aattgaactg
     3481 agggccgacg aaggtgctgt ccaacgagct tgaatattgt ctcaacgatg cgttccatca
     3541 ggcacgtgaa gcgcgtcacg aatacctcac ggtggagcat ctgctgctcg ccattctgga
     3601 tacgccgaag gtccgcgaga tcctgcgggc ctgcggggcg gacctcggca agctgaagca
     3661 ggagctcaaa gaccacgtgg accagtccac gccgcgcctg gaagagggcg aggagcgcga
     3721 ggttcagccc accttgggct tccagcgcgt gctgcagcgc gccgtattcc acgtgcaatc
     3781 gagcggcaag aaggaagtcg gcgtcggcaa tgtgctggtg gcgatcttca gcgagaagca
     3841 gagccatgcc gtcttcctgc tgaaccgcca gcacgtcacc cgcctcgacg tagtgaatta
     3901 catctcgcac ggtctttcga agatcgccga ggagaagacc acgacggaca aggaggatac
     3961 gcagtccgat ggtgagcggg atccggaggg cggcagcgcc ctggagaaat acaccactca
     4021 tctgaaccgc ctggcccagg acgggcgcat cgacccgctc atcgggcgca agctcgaggt
     4081 cgagcgcacg atcgagatcc tttgccgtcg ccgcaagaac aatcccctgt atgtcggcga
     4141 ggccggagtg ggcaagaccg ccattgccga gggcctggcg cggctgatcg tcgagggcaa
     4201 ggtgccggag gtcctctcag actgcaccct ctacgccctg gacatgggcg ccctgatcgc
     4261 cgggaccaag taccgcggtg atttcgagaa gcgcctcaaa ggggtgatca ccgagctgaa
     4321 gaagcagccc ggagccatcc tgttcatcga cgagatccat accgtcatcg gcgccggtgc
     4381 ggcctcgggg ggcgtgatgg atgcctccaa tctcatcaag ccggtcctga ccaacgggga
     4441 aatccgctgc atcggctcga ccacctacca ggaataccgc ggcatctttg aaaaggatca
     4501 cgccctcgcg cggcgcttcc agaagatcga cgtcgtggag ccctcagtcg ccgagacggt
     4561 ggaaatcctg atggggttga agcctcgctt cgaggagcat cacggcatca cctacgccaa
     4621 cgatgcgctc aaggccgctg ccgagctggc cgcgcgccac atcaacgagc ggcatctgcc
     4681 ggacaaggcg attgatgtgg tggatgaggc cggcgcgcgt cagcggctca agcccgtcgc
     4741 cgagcgcgag ccgttgatcg aggtgcgtca catcgaggac atcgtcgcgc gcatggcgcg
     4801 gatcccgccg aagagtgttt ctacctccga tcgcgaggtg ctgcgcaatc tcgagcgcaa
     4861 cctgaagctc gtgatcttcg gccaggacaa ggcgatcgag tctctcgccg catcgatcaa
     4921 gatggcgcgc tcgggcctcg gcgatcagcg caagcccgtg ggcagcttcc tgttcgccgg
     4981 cccgacggga gtcggcaaga ccgaggtcac gcggcagctt gcgatctgta tgggtgttga
     5041 attcctgcgc ttcgacatgt ccgagtacat ggagcgccat accgtctcgc gtctcatcgg
     5101 cgcacctccc ggctacgtcg gcttcgatca gggcggcctg ttgacggagg ccatcaccaa
     5161 gcatccgcat tgcgtgctgc tgctcgatga ggtcgagaag gcgcatccgg atgtgttcaa
     5221 cctgctgctg caggtcatgg atcacggcac gctcaccgac aacaacggcc gcaaggccga
     5281 cttccgccac gtgatcatcg tgatgacgac caacgccggc gcgcaggaga tgagccggcc
     5341 ttcgatcggt ttcacgcaat cggacaacac cagcgatggc atggaagcga tccggcggat
     5401 cttcaccccg gaattccgca accgcctgga tgcggtgatt cagttcgcct cgctcgatcc
     5461 gatcacgatc gagcgcgtgg tggacaagct gctcgtcgaa gtcgagacgc agctggaagc
     5521 gaagggcgtc tcgatcacgc tcgacgatgc ggcgcggcgc tgggtagccg agaagggcta
     5581 cgacccgaag atgggcgcgc gtccgatggc gcggctcatt caggagcaca tcaagcggcc
     5641 gctcgccgag gagctgctgt tcggcaagct ggtcggcggc ggccaggtca ccgtgacggt
     5701 gagcgaggat gggtcgaagc tgaagctgga tagcgttccc acccagcttc ccgaagtgac
     5761 ggcctgagcg gccggggggt ggtgagtcag ggaccggtca acggccccga tagatgattc
     5821 ggcccttggt cagatcgtaa ggggtcattt ccacggtcac ggcatcaccc gtgaggatgc
     5881 ggatgtagtt cttgcgcatc ttcccggaga tgtgcgcagt cacgacgtgc ccgttcttga
     5941 gcttcacgcg gaacgtcgtg ttcggcaggg tctcgacgac ctcgccttcc atctggatgg
     6001 catcttcctt cgacatacgg tccttaccgg cttgaggggc gcgaattgca gcatatccct
     6061 cctacgcaat gcaattttgc cgcacgggcg gcagtgggtc gggcggagcg gggccctagc
     6121 tgatccgcag agcctgcgtg ccgatgtgct tgcgcagcag cgagaggaac tggctgcggg
     6181 cgatggcgcg gctgcccagg ctctgcaggt gccgtgaggc gagctggcag tcgatgacgg
     6241 cgatgccgtt gtcgcgacac aggctgacca ggtgcgctag tgccactttc gaggcatcac
     6301 gctcccggct gaacatcgac tcaccgaaga agacgctgcc gaggcgcaca ccgtacaggc
     6361 cccccaccaa cactccctgg cggtacgcct cgatgctgtg cgcgtactcg agccggtgca
     6421 gctgcagata ggcattgcgc atctccgggg tgatccaggt tcccggactg tggggtcgtg
     6481 gcgcggcaca actgtcgatc acggccgcga aatcccggtt gacggcgagg gagaaccccc
     6541 cgttgcgcag cgtcttggcg aggctgcggg cgcagcgaaa ttcctccggg aacagcaccg
     6601 cccggggatc cggcgaccac cacagcaccg gctgtcccgg ggagtaccag ggaaagatgc
     6661 cgcgccggta ggccgccagc agtcgggccg gggagaggtc gccgccggcg gccagcaatc
     6721 ccggcggatc ctccagagcc tgctcgggcg ccgggaagct atccggggcg tcctgcgggg
     6781 acagccaggt gatggatctc aagcgatttc cccaccgccg gccgagcgct tgtcgcgata
     6841 ggggatgtgc tggcgaacct cctgggcata ggcatccacg gaggccccct cctgctggag
     6901 gtactcggca atggccgtgc ggaagcggcg attcgcgatg aaatggcccg accaggtgag
     6961 ggtcggctcg aagccgcggc tcaccttgtg ctctccctgg gttccaggct cgaaccggcc
     7021 gacgccgcgc tcgatgcaga attccacgcc ctggtggtag cacgcttcga aatgcaggct
     7081 gttgtactcg gcggaggcac cccagtagcg gccaaagagc gcctgcggac accagaagaa
     7141 gatcgccgcc gctaccgggc ggctgcggtg tacggccagc ttgaccatca gggcatcgcc
     7201 gagcgtgcgg gccacttcgc tgaagaacgc gcgtgtcagg tagggctcat gtccatggcg
     7261 cagaaaggtg tcgcgatgga attcgtacac gcagtcgagc agccgctcat ccagatccgc
     7321 tcccatgcgt gtctcgaaca cgatgccggc ctcggccaca cggcgccgct cgcgccgggc
     7381 tttcttgcgc ttctccgcgg tgaaggtgtc caggtactca tcgaagctcg catagccttg
     7441 attgcgccag tggaactggc agtcccgacg ggtgagccag cccgcgtgct cgcaagccgc
     7501 tcggcccggc tcgtcgagga aaagcgcgtg gaccgaggag agggcatggc cttgcgtgtg
     7561 attttccagc tcggtgagga gtcggcgcgt cgtggccgtg ctgtcgagat ccggacggac
     7621 cagtagccgc gcgccggtgg caggggtgaa cggtacagcg acagtgagct tggggtaata
     7681 gcgcagtccg aagcgggaat aggcctgcgc ccaggcgaag tcgaacacga actcgccgta
     7741 ggagtggccc ttggcaaagg ccggcactgc cgcggcgagt cccgacggat cgctcaggct
     7801 cagatagcag ggattccagc cggtgccggg cccgatgcag ccggtgtgct ccagtgcggc
     7861 caggaactca tggcgcagga agggattgcg gtccgggacg agcgcattcc actgctgcgg
     7921 gctgagctca tcgatgcttt tgtgtactcg cgtgcgcatc cgtggccaca tccagggtct
     7981 caagataccg gtccgcatcg agtgccgcca tgcagcccgc gcccgccgag gtgatggcct
     8041 gccggtagac gtgatcggcg acatctccgg cggcgaacac gccgggaatg gtggtggcgg
     8101 tggcattccc ctcgagcccg ctgcgggtgc ggatgtagcc ttcgcgcatc tcgagttgtc
     8161 cggcgaagat cgcggtgtta ggcgagtggc cgatggcgac gaagagcccg gtgatgtcga
     8221 tctcgcgcgt gcccggcccg tccgtcgagc gcagccgcat gccctggact ccgtgttgat
     8281 cgccgaggac ctcctccacc gtgtggctcc agatcacgga gatctttcca ctctcgacct
     8341 cgcggaacag ccggtcctgc aggatcttct ccgcacgcag cttctcgcgc cggtgcacga
     8401 gcgtcacgtg ctgggcgatg tgcgacaggt aaagcgcttc ctccacggct gtgttgccgc
     8461 cgccggccac agcgaccttg tggccgcgga aaaagaatcc atcgcaggtg gcgca
//
LOCUS       DASRHN010000296         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2406059, whole genome shotgun sequence.
ACCESSION   DASRHN010000296 DASRHN010000000
VERSION     DASRHN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406059"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="VFB37_11905"
     CDS             complement(<1..1817)
                     /locus_tag="VFB37_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZO23195.1"
                     /translation="MLGLALRTLGREWRSGELGVLLLALTIAVGALTGVGFLVSRISA
                     AVAIQATEVLAADIRLGSPQPIKDYYFQEAQRRGIRTARISTLLSVVFNGERSQLSNV
                     QAVGEGYPLRGELLVADELFAKGVAARGVPGQGEIWPDSRLLAAVGAKVGDSLSIGAT
                     TFKVSKVLIARPDQGGTFADLAPTAIMNAADLPATQLIQPGSRVSYSALFAADRDRIE
                     TFKTWLRAHKQRSERLRDISETSPQIRNAVDRAGRFLSLASLVSVLLSAIAVAMSARR
                     YVHRHLDAVALLKTLGATRAFTLSVSVVQLLIIAIGAAALGSVLGYLAQEWLIHTVKD
                     LLATQLPAADLTPIAIGFMTALAVLCGFALPPLLQLARVPAIRVLRRDIGPPPPLVIV
                     AFGPAVAVVVLLIYWVVLDWRLFWGFTIGLAAFVLLLTGAGAVLVSFAGQLRGQVGVA
                     WRYGVANLSRRRAESLVQIVAFGMGIMVLLLLAIVRGDLNSDWRRSLPENVPNYFFVN
                     IPPAEREAFIDFLHAHGAQTARVLPMIRGRLVAIDGRPLERMRIGGENGDNLATREQN
                     LTWAAELGDDNRVVDGRWWTPEDFGKPLVSLSTEMQESLG"
     gene            complement(1864..>2601)
                     /locus_tag="VFB37_11910"
     CDS             complement(1864..>2601)
                     /locus_tag="VFB37_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO23196.1"
                     /translation="PSAQAPFSRESVLKAENLTKQVSSPEGPLTIVQEVSLDIAAGES
                     VAIVGASGAGKSTLLALLAGLDLPTTGRVLLAGADLTQLDEDGRARLRAQRVGFVFQS
                     FHLIPALTALENVMLPLELAGRAQARRDALDTLARVGLKERTGHYPRQLSGGEQQRVA
                     LARAFVTQPAVLFADEPTGNLDTTTGARVGELLFELNANSQTTLVLVTHDRELASRCA
                     RTIHMEGGRVIEGVPAVQGGVAQASHG"
BASE COUNT          470 a          875 c          854 g          402 t
ORIGIN      
        1 ccgagcgact cctgcatctc cgtcgagagc gacacgagcg gcttgccgaa gtcctccggg
       61 gtccaccagc gcccgtcgac gacgcggttg tcatcgccaa gctccgcggc ccaggtgagg
      121 ttctgctcgc gagtggccag attgtcaccg ttctcgccgc cgattcgcat gcgctcgagc
      181 ggccgtccgt cgatggccac caggcgcccc cggatcatcg gcagcacccg cgccgtctgc
      241 gccccgtgag cgtgcaggaa atcgatgaaa gcctcgcgct cggccggcgg aatgttgacg
      301 aagaagtagt tgggcacgtt ttccggcagc gatcgccgcc agtcgctgtt caggtcgccg
      361 cgcacgatcg cgagcagcaa cagcaccatg attcccatgc cgaaggcgac gatctggacc
      421 agactctcgg cgcgccggcg gctgagattg gccacgccgt atcgccatgc gacacccacc
      481 tgaccgcgca gttgaccggc gaaggacacg agcacggcgc ccgcgcccgt gaggagcagc
      541 acgaaggcgg cgagcccgat cgtgaagccc cagaacagcc gccagtcgag cacgacccaa
      601 tagatcaaaa ggaccacgac cgccacggcg gggccgaaag cgacgatgac cagcggtggc
      661 ggcgggccga tgtcacgcct gagcacccgg atcgccggca cacgcgccag ttggagaagc
      721 ggcgggagcg cgaaaccaca gaggaccgcg agggcggtca tgaatccgat cgcgatcggc
      781 gtcagatcgg cagcgggcaa ctgcgtggcc agcaggtcct tcaccgtgtg gatgagccac
      841 tcctgcgcca gatagcccaa tacggaacct aacgccgccg cgccgatcgc gatgatgagg
      901 agctgcacga cactcacact cagcgtgaag gcccgggtcg cgccaagtgt cttcagcaga
      961 gctacggcgt cgagatgccg gtgcacataa cgtcgcgccg acatcgctac cgcaatggcc
     1021 gatagcaaaa cgctgacgag gctcgcgagg ctgagaaagc gtccggcgcg gtcgactgcg
     1081 ttacggatct gtgggctggt ttccgagatg tcccggagcc gctcgctgcg ttgcttgtgc
     1141 gcccggagcc aggttttgaa cgtctcgatc cggtcacggt cggcggcgaa cagggcgctg
     1201 tagctgacgc ggctgcccgg ctggatgagc tgcgttgccg gcagatcggc cgcgttcatg
     1261 atcgcagtcg gcgcgagatc cgcgaaggtg cctccctggt ccgggcgggc gatcagcacc
     1321 ttcgacactt tgaaggttgt cgcgccgatg gagagcgagt cgccaacctt ggcgcccact
     1381 gccgccagca gtctggagtc gggccagatc tcgccctgcc ccgggacgcc acgcgcggcg
     1441 acaccctttg caaagagctc atccgctacg aggagctcgc cgcgcagcgg atagccttcc
     1501 cccacagcct gaacattgct gagctgactg cgttctccgt tgaatacaac actcagcaga
     1561 gtgctgatcc gcgcggtgcg gatcccacgg cgctgcgcct cctgaaagta gtagtctttg
     1621 atcggctgtg gcgatcccag ccgaatgtcg gccgcgagga cttcggttgc ctgaatggcg
     1681 acggcggcgc tgatccggct gaccaggaaa ccgacgccgg tgagcgctcc gacggcgatg
     1741 gtcaacgcaa gcagcagaac tcccagctcg cccgagcgcc attcgcgtcc caacgtccgc
     1801 agtgccaaac ctagcattgg attcgcatga cctatgtccg tattgcggtg cagccggcgc
     1861 gcttcacccg tgcgatgcct gcgccacgcc gccttgtacc gcgggcacac cctcgatcac
     1921 gcggccgcct tccatgtgga tggtgcgagc acagcgcgat gccagttccc tgtcatgagt
     1981 cacgagcacg agggtcgtct gcgagttcgc attcagctcg aaaagcaact cgcccacacg
     2041 ggcgcccgtg gtggtatcga ggtttcccgt cggctcgtcc gcgaagagca ccgccggttg
     2101 cgtcacgaat gccctcgcca gcgcgacccg ctgctgctcg ccgcccgaga gctgccttgg
     2161 ataatggccc gtgcgctcct tcagcccgac gcgcgccagc gtatcgagcg catcgcggcg
     2221 ggcctgcgcc cggccggcca gctccagagg cagcatgacg ttctccagcg ccgtcagcgc
     2281 cggaataagg tgaaaggact ggaagacgaa gcccacgcgc tgagcgcgca ggcgggcgcg
     2341 cccatcctca tcgagctgcg taagatcggc tccggcgagc aggacgcggc ctgtggtggg
     2401 cagatcgagg ccggcgagca gcgccaggag agtggatttt ccggccccgg aagcgccgac
     2461 gatcgcgacc gattcgccgg cggcaatatc cagcgagacc tcctgcacga tagtcagcgg
     2521 gccttcggga ctgctaacct gcttggtgag gttttcggcc ttgagaacac tttcgcgact
     2581 gaagggcgcc tgggcgctcg g
//
LOCUS       DASRHN010000297         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1423620, whole genome shotgun sequence.
ACCESSION   DASRHN010000297 DASRHN010000000
VERSION     DASRHN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423620"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFB37_11915"
     CDS             <1..399
                     /locus_tag="VFB37_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZO23197.1"
                     /translation="ASEDGVVRVRSAYPFPETVERLKSNVKAKGIMMFSQIDQARLAA
                     DAGIALRPSALLVFGNPPLGIQFITSKAEAGLDWPVRLLVYEDESGQTWAAYSDFDWI
                     ARRHGIKDREQAFKMAASVIASIAAAVATK"
     gene            575..1216
                     /locus_tag="VFB37_11920"
     CDS             575..1216
                     /locus_tag="VFB37_11920"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HZO23198.1"
                     /translation="MAERLDTYAQSLREINLPMAEAVDRLVARLNQSRLADSAPAVGE
                     AMPTFLLPDHAGHLVGLEELLARGPLAVTFARGHWCPYCRIAVSALAEIADDVAGTGA
                     GIVAIVPDRQEYAAKLRAEAHAPFPILTDVDNAYALSLGLVFWVGEEMEHHMRARDVD
                     LGSSQGNDSWFVPVPATFIVGTDGRIVARHVDPDYRKRMEVEAVLAALRAAGG"
     gene            complement(1225..>2113)
                     /locus_tag="VFB37_11925"
     CDS             complement(1225..>2113)
                     /locus_tag="VFB37_11925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO23199.1"
                     /translation="EMHQVRYFLTVARTLNFTRAAEQCNVSQPALTRAIRQLEEELGG
                     DLLRREGKLSHLTELGQRMLPLMQQCYDSALAAKSLASSLKKGVANPLALGLSHTIDM
                     RLLTPVLTELQRAVAGLQIKVLRGNAGEIAEALQKGRVEFAIAGPLGQTWDRLEAWPL
                     FDERFELAINSDHALAAAEAVDACKLAGQRLLISEQCEQAHSLAQILTERGIAGGTSH
                     SLNSLDDVVALVAANLGVALLPQSAGHYAQLRRIAVADLDLARKVSLYGVAGRQRSAG
                     GDALMKLLRARTWPDRAVA"
BASE COUNT          369 a          692 c          700 g          352 t
ORIGIN      
        1 gcctccgagg acggcgtcgt gcgcgtcagg agcgcttatc cgttcccaga gaccgtggag
       61 cgcctcaaga gcaacgtgaa ggcgaaaggc atcatgatgt tctcgcaaat cgaccaggcc
      121 cggctcgccg cggacgccgg catagcattg cggccgtccg cgctgctcgt attcggcaat
      181 cctccgctcg gcattcagtt catcacgtcc aaggccgagg ccggcctcga ctggcctgtg
      241 cggctgttgg tctatgagga tgagagcggg cagacctggg ccgcctacag cgatttcgac
      301 tggatcgccc gccgccacgg catcaaggac cgcgagcagg ccttcaagat ggccgcgagc
      361 gtgatcgcat ctatcgccgc cgccgtcgcg accaagtgaa tgcggcagag cgattggaag
      421 gctcgagcgg gatgcgcgag cacgcaatct tgcgctcccg aaacggagat atatcctgct
      481 tcaacattat tcgaccgtcc gtgccgacga gagtgtgatg ccagccagga ccatagccga
      541 agcattcgag catgcccgct ccctcgacgc accgttggcc gagcggctgg acacctacgc
      601 ccagtccctc cgcgagatca atctgcccat ggccgaagcg gtcgacaggc tggtggcgcg
      661 cctcaatcag agcaggctgg cggacagcgc accggccgtg ggcgaagcca tgccgacctt
      721 cctgcttccg gaccatgcgg gccacctggt cgggctcgaa gagttgttgg cgcgaggacc
      781 gctggccgtc acctttgcgc gcgggcactg gtgcccctac tgcaggatcg cggtgagcgc
      841 actggccgag atcgccgatg atgtggcggg gaccggcgca ggcatcgttg ccattgtgcc
      901 ggaccggcag gaatacgccg ccaagctgcg cgcggaagcc catgccccgt tcccgatcct
      961 caccgatgtc gacaatgcct atgcgttgtc gctcgggctc gtgttctggg tcggcgagga
     1021 gatggagcac cacatgcggg cgcgcgatgt cgatctcggc agctcgcagg gcaacgacag
     1081 ctggttcgtg cccgtgcccg cgaccttcat tgtcggcacg gacggcagga tcgtcgcccg
     1141 ccacgtcgat cccgactacc gcaagcgcat ggaggtggaa gccgtcctgg cggcgctccg
     1201 cgcagccgga ggttgaccga gagatcacgc aacggcgcgg tcaggccagg tgcgggcacg
     1261 caagagcttc atgagggcat cgccacccgc cgagcgctgg cgacccgcca caccgtagag
     1321 cgataccttg cgtgcaagat ccaggtccgc aacggcaatt cgccgaagct gtgcatagtg
     1381 accagcgctc tgcggcagca gcgcgacccc caggttggca gcgaccaggg ctacgacatc
     1441 gtcgagcgaa tttaaactgt ggctggtgcc gccggcaatc ccgcgctcag tcaagatctg
     1501 ggccaggctg tgcgcctgtt cgcactgctc gctgatcagc aggcgttgcc cggcgagctt
     1561 gcatgcatcc acggcttcgg cggcggcaag cgcgtgatcg ctattgatgg cgagttcgaa
     1621 cctctcgtca aacagcggcc aggcctcgag ccggtcccac gtctgcccga gcggaccggc
     1681 gatggcaaat tccacccgcc ccttctgaag cgcctcggcg atctcgcctg cgttcccgcg
     1741 cagtaccttg atctgcaggc ccgcgacagc gcgctgcagc tctgtgagca ccggcgtgag
     1801 caggcgcata tcgatcgtgt gcgaaagccc caaggccaga ggattggcca cgcccttctt
     1861 cagggatgac gcgagtgact ttgccgccag agcactgtcg tagcattgct gcatgagggg
     1921 gagcatgcgc tgcccgagct cggtcaggtg cgagagcttg ccctcgcgcc gcaggaggtc
     1981 cccacccagt tcctcctcga gctggcggat tgcgcgtgtc agggcgggct gggagacgtt
     2041 gcactgctcg gccgcgcgcg tgaagttgag ggtgcgcgca acggtgagga agtagcggac
     2101 ctgatgcatt tcc
//
LOCUS       DASRHN010000298        16503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1088837, whole genome shotgun sequence.
ACCESSION   DASRHN010000298 DASRHN010000000
VERSION     DASRHN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16503
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088837"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="hslV"
                     /locus_tag="VFB37_11930"
     CDS             <1..436
                     /gene="hslV"
                     /locus_tag="VFB37_11930"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZO23200.1"
                     /translation="RKVRRLYNDSVLAGFAGGTADAFTLFERFEGKLEKYGNLTRAAI
                     ELAKDWRSDRFLRRLEALLLVADLSKILLISGNGDVIEPDHDVAAIGSGGQFALAAAR
                     ALLEGSELDARSIVERSLGIAADICIYTNRNLVIDELKQSVA"
     gene            436..1773
                     /gene="hslU"
                     /locus_tag="VFB37_11935"
     CDS             436..1773
                     /gene="hslU"
                     /locus_tag="VFB37_11935"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZO23201.1"
                     /translation="MSSLTPRQIVQELDKHIIGQGAAKRAVAIALRNRWRRMQVDEPL
                     RQEITPKNILMIGPTGVGKTEIARRLAKLANAPFVKVEATKFTEVGYVGRDVDSIIKE
                     LVDVAVKMTREQEVEKVRQRAADAAEDRVLDTLLPKPRMMGFSTDEPAQPRDAETRQK
                     FRKMLREHELDEREIEIEVRALPMGVEIMAPPGMEEMQQQLQSMFQNLGGNRTRARKV
                     KVREALRLLTDEEAAKLINEDELKIQALANAEQNGIVFIDEIDKVTRRQETAGADVSR
                     EGVQRDLLPLVEGSTVATKYGPVKTDHVLFIASGAFSLSKPSDLIPELQGRLPIRVEL
                     ESLKVGDFVRILTEPDASLTTQYSALMGTEGVRVDFAPDGVRRIAEIAFQVNERTENI
                     GARRLHTVMERLLETVSYEATNQNGSTVSVDARYVDEHLGNLVKDEDLSRYIL"
     gene            complement(1761..2669)
                     /locus_tag="VFB37_11940"
     CDS             complement(1761..2669)
                     /locus_tag="VFB37_11940"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZO23202.1"
                     /translation="MRAPSGRLLQERPRHWHARAMLKVFWLVLRDSAMGWLDHQAART
                     GAALAFYTVFSLAPVLTLSIAMAGFFFGEAAARGEVVDQIGDLIGPEGAHAVQTVLQN
                     ADRPGAGFVATVVSLVTLILGATTALAELKSGLDQIWNVPLERRQGLWYVIRTRLLSV
                     GLILALGFLMLVSLVISAALAALARVWRGEQLLDVLLAWINFLLAFVLVAALFATIYK
                     VLPSVRIAWRDVAIGSLVTAFLFSAGKFAIGVYIGNSGIASTYGAAGSVILVLLWVYY
                     SAQILLFGAEFTRAYAYRLGSFAHRM"
     gene            complement(2666..3490)
                     /locus_tag="VFB37_11945"
     CDS             complement(2666..3490)
                     /locus_tag="VFB37_11945"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZO23203.1"
                     /translation="MTVHRTRGPLGPARNVLERLRRREHAGRDLLRIASYNIHGCVGA
                     DSRYDVERVAQVICELGCDTVGLQQVDGRPGLLTDSMQLEFLASSTGMSAVGNVMRIG
                     TGQQYGNALLTLREVMAVHQYDVTFGGCEPRNLLDVALRACDGVIRVIVTHLGQRAAE
                     RRFQVKKILTLLRTIPIGQPVVLLGDINEWLPLGRPLRWLNRMLGRAPTQRSFPVWAP
                     LFAFDRLWTRSPAMVLTLESHRTSLSRLASDHFPVKAVIAPNIQVQRSHLRYLDVP"
     gene            complement(3594..5549)
                     /locus_tag="VFB37_11950"
     CDS             complement(3594..5549)
                     /locus_tag="VFB37_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZO23204.1"
                     /translation="MRRTRTQRASLRRRPRRSRIPAHTGELGPRHAPSRRFVRGRSAA
                     DAYPPIADYALIGDCHSAALVARSGSIDWCCLPRFDSDSCFGRLLDWGRGGYFAICAP
                     RGARVHREYLPDTLILLTTYRHGRNEARVIDFFSMRMGGRRRPRRELVRIIEGVRGKM
                     EFEISIVPRLDYGDVKPWVYPSGDHAYFAVGSNTGLRIFGDVSLQVKADHDLHATVRV
                     KAKQRLHLGLQYFRPEKAHTVPQRRKAGAELRAHFHETARWWRQWARRIVYTDGPGPG
                     ILRSAVTLKALTFAPTGAIIAAPTTSLPEHVGGERNWDYRYCWIRDSIFTCWALTNSG
                     AGAEADGVRRFIQRTAAGNADELQVLYAVDGKRRLTEVRLSTLDGWRGSRPARIGNAA
                     ERQFQADMYGLVLELSWRWTTQGHRPSEPYWRFLRQIVEAAILRGPEPDRGIWEIRSR
                     PLHFVHSKVMCWAAVDRGIALAEAYGFDAPLAAWRQARQDMRNQIERRGVDHGRNIFV
                     RSFGSRDVDAALLLLPLTDFVAYDDERMVRTTDAIRKELTHDGLLLRYRSEDGLRGHE
                     GAFLACTFWLVECLARQDRKREARTVFRRACACANDVGLFAEQFSRKKGQMLGNFPQG
                     LTHLAHINAALALSSMPLPQPPAGSTR"
     gene            complement(5565..6248)
                     /locus_tag="VFB37_11955"
     CDS             complement(5565..6248)
                     /locus_tag="VFB37_11955"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006822499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZO23205.1"
                     /translation="MTTYVLPDLQYDYGALEPHISARIMQLHHDKHHKTYVDGANQAL
                     EQLEEARTRGDFGRIAAVEQALAFNLSGHVLHSIFWCNLSPDGGGKPSGELASAIDRD
                     FGGFDHFKQQLTKAAATCMGSGWGALTWDSLAGRLLTVQIHDHQSHTIQGGLPLLVID
                     AWEHAYYLQYQNEKAKFFDAVWNVWNWKDVAERFERARAFELLGSGSGQSGQRTQKRP
                     QPQAPAGRA"
     gene            complement(6372..6635)
                     /locus_tag="VFB37_11960"
     CDS             complement(6372..6635)
                     /locus_tag="VFB37_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23206.1"
                     /translation="MKRSVLLLCLSALALVACSKQQSTSPSTPSNDQSATAPATPPSD
                     SSSTAPATPPSDQASPSTTPPADSSGSATPPPPPPDQNNPPPK"
     gene            6831..7922
                     /locus_tag="VFB37_11965"
     CDS             6831..7922
                     /locus_tag="VFB37_11965"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HZO23207.1"
                     /translation="MRHAAITGWGKCLPPAVLTNQDLATFLETSDDWIVSRTGIRERR
                     ISHVPLGELGYVAAANALAAAGLSGREVELVVFGTCSHDDQVPNMASGVQLRIGAASA
                     AAMDVNTACTSFLYALSTATALIGTGVVNNALVIGAELISPFMDWTDRDVAVLFGDGA
                     AAVVLQATEEKEGVLAERLGCHAEARESLRVHGMGGRYANRGILYGAVRWQFEGQEIF
                     KRAVQGMGAASEAALARLGLRGDAVDLVVPHQANLRIIEALAKRMHIPMERVFINIER
                     YGNMSAATVPVALCEAVEEGRVRPGSLLLMPGFGGGLSYCAHLLRWGKRTTPQSTSGV
                     TLPPAQRSALDIVRALMAAKKQLTHAEPD"
     gene            complement(8046..8159)
                     /locus_tag="VFB37_11970"
     CDS             complement(8046..8159)
                     /locus_tag="VFB37_11970"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HZO23208.1"
                     /translation="MGKGDARSRRGKIYKGSFGKSRPKDPAKKRKRAAAKK"
     gene            complement(8250..9530)
                     /locus_tag="VFB37_11975"
     CDS             complement(8250..9530)
                     /locus_tag="VFB37_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23209.1"
                     /translation="MHARREHATASPDVRVRSFTAHWRLGILCAALVALSPLIAQATD
                     WELDLDARLVSSDGRASFTQGGLGTLRYGDRQSGPRLGRARFAISQALGEVWALHLDA
                     SNWGDRDRNPVDLTEAYLLFRPYPVAGYRFRLKAGAFHAPISLENRAAGWESPYTLSY
                     SAINTWLGEELRTIGVEGQLDWLGTRAGQQWDVGLTGGVFGWNDGAGTVVATRGFALD
                     DRQTTLFGRVGATGAAPLAPIDVFREIDDRPGYYGGLEAKYLDRLVVRFMHYDNRADP
                     EAFDSIVRQFAWDTHFNAAGARAEMANGWTALAQWLDGETSIEPGGQYDYWPFNAKFA
                     MLSRQLGKHRLSLRYDTFRVQSELPNGAGAQNGHAWTAAYVYEPDSHWRLTLEWLRVD
                     SNTTNRMLELHEAMFARETQVQLAARYALGPLSN"
     gene            complement(9537..10211)
                     /locus_tag="VFB37_11980"
     CDS             complement(9537..10211)
                     /locus_tag="VFB37_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HZO23210.1"
                     /translation="MRPSARHARAFPAWFAALLVGALLHGTMARSATIVLAVQTADAR
                     PLPGAVITLHNLSVTAHPSVPVQAVMDQVNRAFEPDLLVVPVGSSVEFPNSDSVSHQI
                     YSFSPAKRFQLPLYRGKPYPPVHFDQTGLVILGCNIHDSMLAYVLVTDAPYFGRTDAS
                     GSWSAEVPRGTYKVTVWHPRMQQGSELEREISIGDSDRASLSLRVAKALQPAPLEGAH
                     HSWDTY"
     gene            10283..12676
                     /locus_tag="VFB37_11985"
     CDS             10283..12676
                     /locus_tag="VFB37_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO23211.1"
                     /translation="MGSFRKRLLVLIIGLVVVTQTVTLAAVLASTAHTVETRAAEQLR
                     SGASFAQQLIRFRAGQLATGVAVLAADFGFREAVASGDAPTIISAATNNAQRIGADMV
                     LVLDTRGKVLASTGSEGPARGISLDSLVSQSRAQREQPTFVVLRGRPYQFFLAPVRAP
                     ETIAWVAMGFVLDDLLAQKMRALVGADVSLVTHGSDGGPARTASTLAANQWLAAGTLA
                     LMSTASDAAPRVMRLSDTDYLSFARRIDGPGDSVDVIVQKPMHEVLAPYRDVRDALLL
                     IDGIALALAAIIGTLLGRSAGRPIAELVRAAERIRQGRYDVAVKASGGEEFRSLATTF
                     NTMQKNIAEREADITYLAYHDPVTGLPNRAFAEKRLEALLSGAPPTLALIVINLRNTR
                     EINASLGHHVGDNVLREAARRLRTNIAPDDLAVRLAESQFLLIARGCSRERAPLYADQ
                     MAGVVRAGFHLEEMSLDLDVAAGVCLCPDHGAQAEELLRRAQIAVEDADEARIRVSMY
                     RPGGDEAHRRRLQLITDLRGAIDQNGLTLVYQPKVTMASRSVTSLEALLRWTHPQLGP
                     VGPAEFVPLAERTGSARRLTSWVLGAAIRQLGEWHREGLEVELAVNLSAPDILDPGLG
                     DEIMQTLRAERVAATALLLEITESAVMVDPHLAARNMQLLRVAGVRFAIDDFGTGHSS
                     LSQLRLLPVDELKIDRSFITHARQGSDTATIVTSTIELGHSMGLRVVAEGVESPEAWN
                     LLRQLGCDFAQGYLISRPLVAADVPGFLRSANRLLPASDSTVQQIRALQQLAANERR"
     gene            complement(12696..13751)
                     /locus_tag="VFB37_11990"
     CDS             complement(12696..13751)
                     /locus_tag="VFB37_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZO23212.1"
                     /translation="MTFIAKPKVAHPSLPKNALGLTRRDYEGALSTLCAGCGHDSITA
                     AITEACWDLSIEPQNLVKLSGIGCSSKTTAYFVGGGHGFNSVHGRMPSIAMGANAANR
                     RLHFIGVSGDGDTLSIGLGQFCHAIRRNLNMLYIVENNGVYGLTKGQFSASADVGTKA
                     KKGETNFQPPIDPVLLAMTLGASFVARSFSGDKRQLVPLIQAGLRHNGFALIDVLSPC
                     VTFNDHEGSTKSYAYTREHYEPAVHADFVPLEKEITAEYAAGEALPVVMHDGSRIVLR
                     KCEAGYDPTNRSAAQVQIQERMKAGEYLTGLLFVEPSQSEFHEANGTPDEPLNSVPYE
                     KLSPGPKGMEKILARYR"
     gene            complement(13748..15625)
                     /locus_tag="VFB37_11995"
     CDS             complement(13748..15625)
                     /locus_tag="VFB37_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZO23213.1"
                     /translation="MSASTAARVNDFVIKIATVNGTGSASANTLLMKSIFRSGIPVMG
                     KNYFPSNIQGLPTWYEIRVTQDGHVARSGRVDIMVAMNAETYSRDVKEVATGGYLIYD
                     STWPRPALLKRDDITVIGLPLARLCNENFNGVRTRILMKNICYAGALAALLDLDLDRI
                     RELLAQTYAKKPQLVDSNMKAIQLGYDCARQACSCPLPLHVQKMDRTSGYIMIDGNTA
                     AALGCVYAGATVAAWYPITPSTSLMDAYKGFCDRLRVDPDTGRRNFAFIQAEDELAAI
                     GMVIGAAWNGARAFTATSGPGISLMNEFLGLAYYAEVPAVIFDIQRVGPSTGMPTRTQ
                     QCDIMACAYASHGDTRHVCLYPANPEECFYLGAQAFDLCERLQTPVLVLSDLDIGMND
                     WMCPDLKWDESYRPDRGKMLGREEVMKLEKFYRFLDRDGDGIGYRTLPGVHPKAAYFT
                     RGSGHTQYGVYTEDPVEYQIVVDRLLGKWNTAKKLVPRAVIDSTAGSSIGIVSIGSCD
                     GAIREAMGVLKEAGVEVDYMRVRAFPFSEDVESFLAAHTILFVVEQNRDAQFKSLLTL
                     ETGVAKDKLHSLLHYSGLPISSTFIVEGVLAQVGSRPRKQAEHGERKQVERPRMAEGP
                     R"
     gene            complement(15649..>16503)
                     /locus_tag="VFB37_12000"
     CDS             complement(15649..>16503)
                     /locus_tag="VFB37_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZO23214.1"
                     /translation="ELDAKGRITAERTVGEEFLPADDVILAIGQENAFPWIERDLGIE
                     FDKWDVPQVDPLTFQSTRPGIFFGGDAAFGPKNIIWAVEHGHQAAISIHRHCEGLPIS
                     ERLPPGVKLQSRKMGMHEWSYANDYTPLERRLMPHVSLKERFKKLNIEVELGFTLEQT
                     TAEVQRCLNCDVQTVFAAKLCIECDACIDICPVDCLTITHNGEEAELRSRLKAPAKNL
                     AQPLMVSSALPQTGRVMVKDEDLCVHCGLCAERCPTAAWDMQKSYVSWPHAVDDAQAL
                     HAKAPKIA"
BASE COUNT         2997 a         5323 c         5322 g         2861 t
ORIGIN      
        1 ccgcaaggtg cgccgtcttt acaacgacag cgtgctcgcc ggattcgccg gcggcaccgc
       61 tgatgccttc actctgttcg agcgcttcga gggcaagctc gagaagtacg gcaatctcac
      121 gcgcgcggcc atcgagctgg cgaaggactg gcgctcggac cgcttcctgc gcaggctgga
      181 ggcgctgctg ctggtcgcgg atctgtcgaa gatcctcctc atttccggca atggcgatgt
      241 gatcgagccg gaccatgacg tggcggcgat cggttcgggg ggccaattcg cgctcgccgc
      301 ggcacgagcg ctgcttgagg gctcagagct cgatgcgcgc agcatcgtcg agcgctcgct
      361 cggcatcgct gcggacatct gtatctacac gaaccggaac ctggtcatcg acgagctcaa
      421 gcagtcggtc gcctgatgtc ctcactcacc ccccgccaga tcgtccagga gctggacaag
      481 cacatcatcg gccagggcgc cgccaagcgt gcggtcgcta tcgcgctgcg caatcgctgg
      541 cgacggatgc aagtcgatga gccgttgcgt caggagatca cgcccaagaa catcctgatg
      601 atcggtccga ccggcgtcgg caagacggag atcgcgcgtc gcctggcaaa gctggcgaac
      661 gcgcctttcg tgaaggtcga ggcgaccaag ttcacggagg tcggctacgt cgggcgcgat
      721 gtggactcga tcatcaagga gctggtggac gtcgcggtca aaatgacgcg cgagcaggag
      781 gtcgagaagg ttcgccagcg ggcagccgat gccgctgagg atcgcgtcct ggataccctg
      841 ctgcccaagc cacgcatgat gggcttctcc accgatgagc cggctcagcc gcgcgatgcc
      901 gagacgcggc agaagttccg caagatgttg cgtgagcacg agctcgatga gcgcgaaatc
      961 gagatcgaag tgcgggcgct gccgatgggc gtcgagatca tggcgccgcc cggcatggag
     1021 gagatgcagc agcagctgca gtcgatgttc cagaatctcg gcggcaaccg cacgcgcgcc
     1081 cggaaagtga aggtgcgcga agcgctgcgg ctgctcaccg atgaggaggc cgccaagctg
     1141 atcaacgagg acgagctgaa gatccaggcg ctggcgaacg cggagcagaa tggcatcgtg
     1201 ttcatcgatg agatcgacaa ggtgacccgt cggcaggaaa cggcgggggc agatgtctcg
     1261 cgcgagggcg tgcagcgcga tttgctgccg ctggtagagg ggtcgacggt tgccacgaag
     1321 tatgggccgg tcaagaccga ccacgtgctg ttcattgcct cgggcgcctt cagcctctcc
     1381 aagccttcgg atctgatccc cgagctgcag ggccgcctgc cgatccgggt cgagctcgaa
     1441 tcgctcaagg tcggtgactt cgtgcgcatc ctcactgagc cggatgcatc cctgaccacg
     1501 cagtacagcg cgctcatggg cacggaaggg gtacgtgtcg attttgcacc ggatggcgtc
     1561 cggcgcatcg ccgagatcgc cttccaggtg aacgagcgca ctgagaacat cggcgcgcgg
     1621 cgtctgcaca ccgtgatgga gagattgctg gagacggtct cgtacgaggc gacgaatcag
     1681 aacggcagca cggtcagcgt cgatgcccgg tacgtcgacg agcacctggg caatctggtg
     1741 aaggacgagg atctcagccg ctacatcctg tgagcgaatg atcccaggcg atatgcgtag
     1801 gcgcgggtga attcggcgcc gaagagcaga atctgtgctg agtagtagac ccacagcagc
     1861 accaggatca ccgagccggc ggcgccgtac gtcgaagcga taccgctgtt gccgatgtag
     1921 acgccgatcg caaacttgcc ggcgctgaac aggaacgccg tgaccagtga gccgatcgcc
     1981 acgtcgcgcc aggcaatgcg cacgctcggc agtaccttgt agatggtcgc gaatagcgca
     2041 gccacgagca cgaacgccaa caggaagttg atccacgcga gcagcacgtc caggagctgc
     2101 tccccacgcc agacacgcgc cagcgcagcg agcgctgcgc tgatcaccag tgagacgagc
     2161 atcaggaagc cgagcgcgag aatcaggccc accgacagca gccgtgtgcg gatgacatac
     2221 cacaggccct ggcggcgctc gagcggcacg ttccagatct gatcgagccc cgatttgagc
     2281 tcggcgagag ccgtggtggc gcccagaata agcgtcacaa ggctcacgac cgtcgctacg
     2341 aaccctgcac ccggacgatc cgcgttctgc agcaccgtct gcactgcgtg ggccccctcc
     2401 ggtccgatca ggtcaccgat ctgatcgacc acctcgccgc gtgcagcggc ttccccgaag
     2461 aaaaagcccg ccatcgcgat cgacagcgtg agcacgggag ctagcgagaa cacggtatag
     2521 aaggcgagcg cggcgccggt gcgcgcagcc tgatgatcca accaacccat cgccgagtcc
     2581 cgcagcacga gccagaagac cttcagcatc gctctcgcat gccagtgccg tggcctctcc
     2641 tgcagcagcc gaccggacgg ggctctcacg gaacatccag gtaccgcagg tgcgagcgct
     2701 gcacctgaat gttgggcgcg atgacggcct tgaccggaaa gtgatccgaa gccaggcgcg
     2761 agagcgaggt ccgatgcgac tcgagcgtca ggaccatggc cggcgagcgc gtccagagtc
     2821 gatcgaatgc gaataaaggg gcccagaccg ggaatgatct ctgggtgggc gcccggccca
     2881 gcatccgatt caaccagcgc agcggcctgc ccagcggcaa ccattcgttg atgtcgccca
     2941 ggagcacgac gggctgtccg atcggaatag tgcgcaatag cgtgaggatc ttcttaacct
     3001 ggaagcgtcg ctccgcggcc cgctgcccca ggtgcgtcac gatcacgcgg atgacgccat
     3061 cgcaagccct caatgcaaca tccagcagat tgcgcggctc acatccgccg aacgtcacgt
     3121 cgtactgatg cacggccatg acttcgcgca gtgtcaggag cgcattgccg tactgttggc
     3181 cggtgccaat acgcatcacg ttacccacgg cggacatgcc ggtcgagctc gccagaaact
     3241 cgagctgcat cgaatccgtc agaagcccgg gtcggccgtc aacctgctgc agcccgaccg
     3301 tgtcgcaccc cagctcgcag atgacctggg cgactcgctc gacgtcgtag cgggagtccg
     3361 caccgacaca gccgtggatg ttgtaggaag caatgcgcag cagatcgcgc ccggcgtgct
     3421 cacgccgtcg caggcgctcg agcacattgc gggcgggacc taaaggaccg cgtgtgcgat
     3481 gaactgtcat gaccacgctc acgcaaactt cattcccggg cgggcgcgca agctcatgcc
     3541 tccggtccgg agcgccgacc gccacgctat cagcccctta agggtcccat cagtcagcgt
     3601 gtcgagccgg cgggcggttg cggtaacggc atgctgctga gcgcaagcgc ggcgttgata
     3661 tgcgccagat gtgtcaggcc ctgcggaaaa tttcccaaca tctggccttt cttgcgcgag
     3721 aactgctctg cgaacaggcc cacgtcgttg gcgcaggcgc aggcgcggcg gaagactgtg
     3781 cgcgcttcgc gcttgcgatc ctggcgggcg agacactcca cgagccagaa cgtgcaggcc
     3841 aggaacgcgc cctcgtggcc gcgcagcccg tcttcgctgc gatagcgaag caagagccca
     3901 tcgtgggtga gctccttacg tatcgcatcc gtcgtacgca ccatgcgttc atcgtcatac
     3961 gcgacgaagt cggtgagcgg cagcagcagc agcgcagcat ccacatcccg actgccgaag
     4021 ctgcgcacga agatgttgcg gccgtgatcc acgccgcggc gctcgatctg attacgcatg
     4081 tcttgcctcg cctgacgcca agcggcgagc ggcgcatcga atccatacgc ctcggcaagg
     4141 gcgatgccac ggtccaccgc ggcccagcac atgaccttgg aatgcacgaa gtgcagcggg
     4201 cgcgagcgga tctcccagat gccgcggtcc ggctcgggcc cgcgaagaat cgcggcctcg
     4261 acgatctgcc gcaggaatct ccagtagggc tcgctggggc gatggccctg cgtagtccaa
     4321 cgccaactca actccagcac caggccgtac atgtctgcct gaaactgccg ctcagcggcg
     4381 ttaccgatgc gcgccggccg cgagccccgc cacccgtcca gggtggagag cctgacttcc
     4441 gtcagccggc gctttccatc taccgcatac aacacctgca gctcgtctgc attgccagcg
     4501 gcggtgcgct ggatgaaccg cctgacaccg tccgcttccg cgccagcgcc cgaattggtc
     4561 agcgcccagc aggtgaagat cgagtcacga atccagcaat accgatagtc ccagttacgc
     4621 tctccgccca cgtgctccgg cagcgacgtc gtcggcgcgg cgatgatcgc gcccgtgggc
     4681 gcgaacgtca gcgctttgag agtcacggcc gatcgcagga tgccgggccc cggaccatcg
     4741 gtgtagacga tgcgccgtgc ccactgacgc caccagcgtg ccgtctcgtg gaagtgagcc
     4801 cgcaactcgg caccggcttt gcggcgctgc ggaaccgtgt gcgccttctc cgggcggaaa
     4861 tactgaagtc cgaggtgcag tcgctgcttc gctttgacgc gcaccgttgc gtgcagatca
     4921 tgatcggctt tgacctgcag actcacgtcg ccgaagatcc gcagcccggt gttgctgccg
     4981 accgcgaagt acgcatgatc gccggagggg tacacccatg gcttgacgtc accgtaatcg
     5041 agccgcggca cgatcgagat ttcaaactcc atcttcccgc gtacgccctc gatgatgcgg
     5101 accagctctc gacggggccg gcgcctgccg cccatgcgca tggagaagaa gtcgatcacg
     5161 cgcgcctcat tgcgcccgtg ccggtacgta gtcagcagaa tcagcgtgtc cggcagatac
     5221 tcgcggtgaa cccgcgcgcc gcgcggtgca cagatcgcga aatagccacc acggccccag
     5281 tccagaagcc ggccgaagca gctgtcggaa tcaaatcgcg gcagacagca ccagtcgatg
     5341 gaaccggaac gcgcgaccag ggcggcggag tgacagtcac cgatcagcgc gtaatcggcg
     5401 ataggcggat aggcatccgc agcgctgcgc ccgcggacga agcggcgcga aggcgcatgc
     5461 cggggaccaa gctccccggt atgcgcagga atgcgggaac gacgaggccg gcggcgaagg
     5521 gaggcgcgct gcgtacgtgt tctcctcagc gccggcctgt tggctcaggc tctgcccgcg
     5581 ggggcctgcg gctgcggccg cttctgggta cgctgacccg attgcccgct gccgctgccg
     5641 agaagctcga aggcgcgagc gcgctcgaac cgttcggcga cgtccttcca gttccataca
     5701 ttccagacag catcgaagaa cttcgccttc tcgttctgat actgaaggta gtaagcatgc
     5761 tcccaggcgt cgatcaccag cagcggcagt ccgccctgga tcgtgtggga ctggtgatca
     5821 tggatctgca cggtcaacag ccgccctgcg aggctgtccc aggtgagtgc accccagccg
     5881 gagcccatgc aggtcgccgc cgccttggtg agctgctgct tgaagtgatc gaagccacca
     5941 aagtcgcgat cgatcgccga ggcgagctct ccggaaggtt taccgccccc gtcgggcgaa
     6001 aggttgcacc agaaaatgct gtgcagcaca tgccccgaca ggttgaaggc cagggcctgc
     6061 tcgactgccg cgatgcggcc gaaatccccg cgcgtgcgtg cctcctcgag ctgctcgagc
     6121 gcctgattgg cgccatcgac ataggtcttg tgatgcttgt cgtgatgcag ctgcatgatg
     6181 cgcgcggaaa tatgcggctc gagcgcgccg tagtcgtact gaaggtcggg aagcacatac
     6241 gtggtcattt catcactcct gatgagaacg tcgggaatac tccggcggcc cgcgccgcgc
     6301 gagcgcggtg cgggccaccg gagatccgtg acagactgtg ttctaaagca gatgtctgtt
     6361 tcgggaaaag cctatttcgg aggaggattg ttctgatccg gcggcggagg cggcggcgtg
     6421 gctgagccgc tggagtccgc tggcggggtc gtggacgggc tagcctggtc cgaaggcggc
     6481 gtggccgggg ccgtgctcga ggagtcgctc ggcggggtcg ccggtgcggt tgcggattgg
     6541 tcgttgctgg gcgtgctggg cgaagttgac tgctgcttcg agcaggcaac gagggccagt
     6601 gcgctcaggc acaacaacag gacactgcgt ttcatagggt cacctctctg ttacgggtcg
     6661 ccccgggcag actcgccgcc gggggtggac acaacgttcc gtcgcaagac ccgttccatg
     6721 cgtaacagcg gcgtccgcgt ttcgcggcgt gccatatggg caagcccagg cccgaggcac
     6781 ccggcattca ggcctgctat cctcattcaa ctgctggaag gggcactcac gtgaggcatg
     6841 cagccataac aggttggggc aagtgcctgc cccccgccgt gctgacgaac caggacttgg
     6901 ccacctttct ggagaccagc gacgactgga tcgtgagccg gacaggaatc cgcgagcgac
     6961 gcatctctca cgttccactc ggcgagctcg gttatgtagc cgccgcgaac gccctggcgg
     7021 ccgccggcct gagcgggcgt gaggtcgagc tcgtcgtttt cgggacctgc agccacgacg
     7081 atcaggtgcc caacatggcc tcgggcgtcc agctgcggat cggcgcagcc tcggccgcgg
     7141 caatggatgt caacaccgcc tgtaccagtt tcctttatgc cctttccaca gccaccgctc
     7201 tgatcggcac gggcgtggta aacaatgcac tcgtgatcgg ggcggagttg atttccccct
     7261 tcatggactg gaccgatcgg gacgtcgcgg tactgtttgg cgatggcgct gcggcggtgg
     7321 tgctgcaggc aaccgaggaa aaagaaggcg tgctggcgga gcggctcggt tgccatgctg
     7381 aggcccgtga gagcttgcgc gtgcatggga tgggcggtcg ctatgcgaac cgcggcatcc
     7441 tgtacggggc ggtacgctgg cagttcgagg gtcaggagat cttcaagcga gccgtgcaag
     7501 gcatgggggc tgcgagcgag gccgcgctcg cacgtctggg cctgcgcggc gatgcggtcg
     7561 atctggtcgt gcctcatcag gccaatctgc ggatcatcga ggcgttggcc aaacgcatgc
     7621 acatcccgat ggaacgcgtc ttcatcaaca tcgagcgcta cgggaacatg tctgccgcca
     7681 ctgtgccggt ggcgctgtgc gaggccgtcg aagagggtcg cgtccgaccc ggctcactgc
     7741 tgctgatgcc gggattcgga ggcgggctca gctactgcgc gcatctgctg cggtggggca
     7801 agcggaccac tcctcagagc accagcggcg tcactcttcc accagctcag cgctcagcac
     7861 tcgatatcgt ccgcgcgctg atggcggcca aaaagcagct gacccacgct gagccggatt
     7921 aggggctatt cacacccgcg ttggcctcga caccggctac ccacaaggtg tgctttggcg
     7981 agagggtgct tgcaccgcaa gcccggctcg gatgagcggt gaaccctcag cggggatgcg
     8041 caaattcact tcttggcggc agcgcgcttg cgctttttgg ccggatcctt cggccgcgac
     8101 ttgccgaacg agcccttgta gatcttgccg cgccggcttc tggcgtcgcc cttgcccatg
     8161 aacagaactc cacatgcgaa tgagggcggg cagtctagca ttccgtccgc agagtcgctg
     8221 cagatcgcgt cggccgggcg cgccggcagt caattcgaga gcggtccgag agcatatcgc
     8281 gcggcaagct gcacttgcgt ctcgcgcgca aacatcgcct catggagctc cagcatcctg
     8341 tttgtcgtgt tgctgtccac gcgcagccat tcgagtgtga gccgccagtg tgaatcgggc
     8401 tcgtacacat acgctgccgt ccatgcgtgc ccattctgcg cccccgcccc attgggcagc
     8461 tcgctctgca cgcggaaggt atcgtagcgg agactcaatc ggtgcttgcc gagctgtctc
     8521 gagagcatgg cgaacttggc gttgaagggc cagtaatcat actggccgcc gggctcgatc
     8581 gaagtctccc cgtcgagcca ttgcgccagc gccgtccagc cgttggccat ttcagcacgg
     8641 gcccctgcgg cgttgaagtg agtatcccag gcgaactgtc gaacgatgct gtcgaatgcc
     8701 tcgggatcgg ctcgattgtc atagtgcatg aaacgtacga cgagacggtc cagatatttt
     8761 gcttcgaggc caccgtaata gccgggacga tcgtcgatct cgcgaaacac atctatcgga
     8821 gccaaggggg ccgcgccggt tgcccctaca cggccgaaca gcgtcgtctg ccggtcatcg
     8881 agcgcaaagc cgcgggtggc cactaccgtg ccggcaccgt cattccagcc aaacacaccg
     8941 ccagtcaggc caacatccca ttgctgtccg gcacgggtgc cgagccaatc gagctggccc
     9001 tccactccga tggtgcgcag ctcttcaccc agccaggtgt tgatggccga gtacgagagt
     9061 gtgtatggtg attcccaccc cgcggcacgg ttctcgagcg agatcggtgc gtggaacgcg
     9121 cccgctttca gccggaaccg atagcctgcg accggatacg ggcggaacag caggtatgcc
     9181 tcggtcaggt cgaccggatt tcggtcccgg tcgccccagt tggaggcatc caggtgcagc
     9241 gcccagacct caccgagtgc ctgcgagatc gcgaagcgcg ctcggcccag gcgcggaccc
     9301 gactggcggt cgccgtaccg caaggtgccc aacccaccct gggtaaagga cgcgcgcccg
     9361 tccgagctca cgagacgcgc atccaggtcg agctcccagt cggtcgcttg cgcgatcagt
     9421 gggctcagcg cgaccagcgc cgcacagagt atcccaaggc gccagtgcgc cgtgaaactg
     9481 cgcaccctga catccggcga agctgttgcg tgctcacgtc tggcgtgcat ggctgcctag
     9541 tacgtgtccc aggaatggtg tgccccctcg aggggagccg gctgcagggc tttggccact
     9601 ctcaggctga gcgaggcgcg atcactgtct ccaatcgaga tctcgcgctc cagctcactg
     9661 ccctgctgca tgcgtggatg ccagaccgtc actttgtaag tcccgcgcgg tacttccgcc
     9721 gaccaggagc ccgatgcgtc ggtgcgtcca aagtagggag cgtcggtcac caggacgtat
     9781 gccagcatcg aatcgtgaat gttacatccc aggatcacca ggccggtctg gtcgaaatgc
     9841 accggtggat agggcttgcc gcgatagagg ggaagctgaa accgcttcgc aggtgagaag
     9901 gagtagatct ggtggctgac cgagtcgctg tttgggaatt cgacgctcga gccgacgggc
     9961 accaccagca gatcgggctc gaacgcgcga ttcacctggt ccatgaccgc ctgcacggga
    10021 acgctgggat gggccgtcac gctgaggttg tgcagcgtga tcacggcccc cgggagaggc
    10081 cgggcatcgg ccgtctgcac tgccagcaca attgtcgccg accgggccat cgtgccgtgc
    10141 agcagagcgc cgaccagcag cgcggcgaac cacgccggga acgctctcgc atggcgtgcg
    10201 gaaggtctca ttccggcatc cgaactgtcg cggtagatgg ggcacccgcc tacgatacaa
    10261 acaccccatt cgagtctgtt tcatgggaag cttccgtaaa cgacttctcg tattgatcat
    10321 cggcctggtg gtggtcacac agactgtgac gctcgccgca gtgctcgcca gcacggctca
    10381 cacggtcgag acgcgcgcag cagagcagct tcgttccggc gcttccttcg cacagcagct
    10441 catccgcttc cgcgccggcc agcttgccac cggcgtggca gtgctggccg cggacttcgg
    10501 cttccgggaa gcggtggcca gcggcgatgc gccaacgatc atctcggctg cgaccaacaa
    10561 tgcgcagcgg atcggcgccg atatggttct cgtgctggat acacgcggga aggtgctcgc
    10621 ctccaccggc tcggaagggc cagcgcgcgg catctcgctc gactcattgg tgtcgcaatc
    10681 gcgcgcccag cgggagcaac ctactttcgt ggtgctccgc gggcggccct atcagttctt
    10741 cctcgctccc gtacgggctc ccgagacgat cgcctgggta gccatggggt tcgtactgga
    10801 tgatctcctt gcccagaaga tgcgtgcgct cgtcggcgcc gatgtctcgc tggtcaccca
    10861 tgggagcgac ggcgggccgg cccgtaccgc ctcgacactt gcagccaacc aatggcttgc
    10921 ggcgggcacg ctcgcgctca tgtcgacggc cagcgatgcc gcgccgcggg tgatgagact
    10981 cagcgacact gactacctga gctttgcccg gcgcatagac ggccccggcg actccgtcga
    11041 tgtcatcgtc cagaaaccca tgcatgaggt cctcgccccg tatcgcgacg tacgcgacgc
    11101 cctgttgctg atcgatggga tcgccctggc gctcgcggcc atcatcggca cgttgctggg
    11161 tcgaagcgca ggccgcccga tcgccgagct ggtacgggcc gccgagcgca ttcgccaagg
    11221 gcggtacgac gtagcggtca aggcctcggg cggtgaggag ttccgcagcc tcgccaccac
    11281 gttcaacacg atgcagaaaa acatcgccga acgtgaagcg gacattacgt atctcgcata
    11341 tcacgacccg gtcaccggtc tgcccaatcg ggcattcgcc gagaagcgtc tcgaggcgct
    11401 cctctcggga gcaccgccaa cactcgcgct gatcgtcatc aacctgcgca atacgcgcga
    11461 aatcaacgca tcactcggac accatgtcgg cgacaacgtc ctgcgggagg ccgcacgccg
    11521 gctccgcacc aacattgccc cggacgacct tgcggtccga ctggcagaga gtcaattcct
    11581 gctgatcgca cgcggctgct cacgcgaacg cgctccgctg tacgccgacc agatggcagg
    11641 cgtcgtgcgc gcaggatttc atctcgaaga gatgagcctc gatctcgatg tggcggcggg
    11701 tgtttgcctg tgccccgatc atggcgcgca ggcggaggag ctgctgcgcc gcgcccagat
    11761 agcggtcgag gacgccgatg aagcacgcat ccgggtctcg atgtaccgcc ccggcggcga
    11821 tgaggcccac cgtcggcggc tgcagctcat caccgatctg cgcggcgcga tcgaccagaa
    11881 cggcctgact ctggtgtatc agccgaaggt cacgatggcg agccgctcgg tgaccagtct
    11941 cgaagcactg ctgcgctgga cgcatccgca gctcggcccc gtggggcccg cggaattcgt
    12001 gccgcttgca gagcggacgg gcagtgcgcg cagactgacg agctgggtgc tgggcgccgc
    12061 gatccgccag ctcggcgagt ggcatcgcga gggactcgag gtcgagctgg cggtcaacct
    12121 gtccgcgccc gacatcctgg atcctggcct gggcgatgag atcatgcaga ccctgcgcgc
    12181 cgagcgcgtg gcggcgactg ccttgctgct ggagatcacc gagagcgcgg taatggtgga
    12241 tccacacctg gctgcacgga atatgcagtt gctgcgcgtc gccggtgtgc gcttcgccat
    12301 cgatgacttc ggtaccgggc actcctccct ttctcagctg aggctgctgc cggtggatga
    12361 gctgaagatc gatcgctcgt tcatcacgca tgcccgtcag ggaagcgaca ccgcaacgat
    12421 cgtcacctcc acgatcgagc tgggtcacag catgggtctg agagtggtcg cggagggcgt
    12481 ggagagtcct gaggcgtgga atctgctgcg tcagctcggg tgtgacttcg cacagggcta
    12541 cctgatcagc cggccgctag tcgctgccga cgtgcccggc ttcctgcgca gcgcgaatcg
    12601 ccttctgccg gcttccgact caaccgtaca gcagatccgc gcgctgcagc agcttgcagc
    12661 gaacgagcgc cgctgaattc caatcacgag ccccctcagc gatagcgcgc gagaatcttt
    12721 tccattccct tcggtccggg tgacagcttc tcgtacggca cactgttgag tggctcgtcc
    12781 ggtgtgccgt tggcctcgtg gaactcgctc tgcgagggct cgacgaacag caggccggtg
    12841 aggtattcgc ccgccttcat cctttcctgg atctgcacct gcgctgcgct gcggttggtg
    12901 gggtcataac cggcctcgca ttttcgcagc acgatgcggc tgccatcgtg catcaccacc
    12961 ggcagcgcct cccccgcggc gtactcagcg gtgatttcct tctccagcgg cacgaagtcc
    13021 gcatgcacgg ccggctcgta gtgctcccgg gtataggcgt agctcttggt tgagccctcg
    13081 tggtcgttga aggtcacgca cggcgagagg acgtcgatca acgcgaagcc gttgtgccgc
    13141 agacccgcct ggatcagggg cacgagctga cgcttgtcgc cggaaaagct gcgcgccacg
    13201 aacgaggcgc ccagcgtcat cgcgagcagc accgggtcga tcgggggctg gaagttggtc
    13261 tcgcccttct tggccttcgt gccgacgtcg gccgaggcgg agaactgccc cttggtgagc
    13321 ccgtaaacgc cgttgttctc gacgatgtac agcatgttga gattgcggcg gatggcatgg
    13381 cagaactggc ccaagccaat cgaaagcgta tcgccatcgc cggaaacccc gatgaagtgc
    13441 aggcggcggt tggcggcatt cgcacccatt gcaatggagg gcatgcggcc gtggaccgaa
    13501 ttgaagccgt ggccccctcc cacgaagtac gcggtcgtct tggaggaaca gccgatcccc
    13561 gacagcttga cgagattctg gggctcgatc gacaggtccc agcacgcctc ggtgattgcc
    13621 gcagtgatcg aatcgtgccc gcagccggca cacaatgtcg acagcgcccc ttcgtaatcg
    13681 cgccgcgtca gacccagcgc attcttcggc agtgaggggt gtgcgacctt cggcttcgcg
    13741 atgaatgtca tctcggtccc tcggccatgc gtggcctctc gacctgcttg cgctcgccgt
    13801 gttccgcctg cttccggggc cgtgagccga cctgcgccag caccccctcc acgatgaagg
    13861 tcgaggagat cggcaatccg ctgtagtgca gcagcgagtg cagcttgtcc ttggccacac
    13921 cggtctcgag cgtcagcagg gatttgaact gcgcgtcgcg gttctgctcg acgacgaaca
    13981 ggatcgtgtg cgcggccaga aaactctcca cgtcctcact gaacggaaac gcgcgcacgc
    14041 gcatgtaatc gacctcgacg cctgcttcct tcaggacccc catcgcctca cgaatggccc
    14101 catcgcagct tccgatcgag acgattccga tgctgctgcc agccgttgaa tcgatgaccg
    14161 cacgcggaac cagcttcttg gcggtgttcc atttgccgag caatcggtcc accacgatct
    14221 ggtactcgac cggatcctcg gtatagacgc cgtactgcgt gtggccagat ccgcgcgtga
    14281 agtaggctgc cttcggatgc acaccaggca gcgtgcgata gccgattccg tcgccgtccc
    14341 ggtcgaggaa gcgatagaac ttctcgagct tcatgacctc ctcccggccg agcatcttgc
    14401 cccgatcagg acggtagctc tcgtcccact tcagatcggg acacatccag tcgttcatgc
    14461 cgatgtccag gtcggacagg acgagcaccg gggtctgcag ccgctcgcac agatcgaacg
    14521 cctgcgcgcc caggtagaag cattcctcgg gattggccgg atagaggcag acatgccgcg
    14581 tgtcgccatg cgaggcgtag gcacaggcca tgatgtcgca ttgctgagtc cgggtcggca
    14641 tcccggtcga ggggccgaca cgctggatgt cgaagatcac ggcgggcact tcggcgtagt
    14701 aggcaagacc gagaaactcg ttcatcagcg agatgcccgg accggaagtg gcggtgaagg
    14761 cccgcgcccc gttccaggcc gcgccgatga ccatgccgat tgcggcgagc tcgtcctctg
    14821 cctggatgaa cgcgaaattg cgccggcccg tatccggatc cacgcgcaag cggtcacaaa
    14881 atcccttata ggcgtccatc agcgaggtgg acggcgtgat cggataccag gccgcaaccg
    14941 tcgccccggc gtacacgcaa ccgagagcgg cagccgtgtt gccgtcgatc atgatgtaac
    15001 cggaggtccg gtccatcttc tgcacgtgca acggcagcgg acagctgcag gcttgcctgg
    15061 cgcagtcgta gccgagctgg atggccttca tgttgctgtc cacgagctgc ggcttcttcg
    15121 cgtaagtctg cgccagcagc tcgcggatgc ggtccaagtc cagatcgagc aatgccgcca
    15181 aggctcctgc gtagcagata ttcttcatca ggatgcgcgt gcgcaccccg ttgaagttct
    15241 cgttgcacag ccgcgcaagc ggcaaaccga tgacggtgat gtcatcgcgt ttcagcagcg
    15301 ccggacgcgg ccaggtggag tcgtagatca ggtagccccc ggtggcgacc tccttgacat
    15361 cgcgcgaata cgtctccgcg ttcatcgcca ccatgatgtc cacccgacct gagcgggcga
    15421 catgaccatc ctgtgtcacg cggatctcgt accaggtcgg cagtccctga atgttcgagg
    15481 gaaagtagtt ctttcccatg accgggattc cgctccggaa gatcgacttc atgagcagcg
    15541 tgttggcact ggccgagccg gtgccgttca cggtggcgat cttgatgacg aagtcgttga
    15601 cgcgtgcggc agtggatgca gacatgcttt tcctggactg ttcggctatc aggcgatctt
    15661 gggtgccttg gcgtgcaggg cctgcgcgtc atcgacggca tgcggccagc tcacgtacga
    15721 cttctgcatg tcccacgccg cggtcgggca gcgctcagcg cacaatccgc agtgaacgca
    15781 cagatcctcg tccttcacca tcactcgccc ggtctgagga agggcgctgg acaccatgag
    15841 cggctgcgcc aggttcttcg ccggcgcctt gagccgcgac cgcagttcgg cctcctcacc
    15901 gttgtgcgtg atggtcaggc aatcgaccgg acagatgtcg atgcaggcat cgcattcaat
    15961 gcacagcttg gcggcgaaca ctgtctgcac gtcgcagttg agacagcgct gaacctcggc
    16021 cgtggtctgt tccagcgtga acccgagctc cacctcgata ttgagcttct tgaatcgctc
    16081 cttgagggag acgtgcggca tcaagcggcg ctcgagcggg gtgtagtcat tggcgtagga
    16141 ccactcgtgc atgcccatct tgcggctctg gagcttcacc ccggggggaa ggcgctcgct
    16201 gatgggcagg ccctcgcaat ggcgatggat cgagattgcc gcctgatggc catgctcgac
    16261 cgcccagatg atgttcttcg gaccgaacgc ggcgtccccg ccgaagaaga tacccggccg
    16321 ggtggactgg aaggtcagcg gatcgacctg cggaacatcc cacttgtcga actcgatgcc
    16381 gagatcgcgc tcgatccacg ggaatgcgtt ctcctgaccg atggccagga tgacgtcatc
    16441 cgccggcaga aactcctcgc caaccgtgcg ctccgcggtg atgcgaccct tcgcatccag
    16501 ctc
//
LOCUS       DASRHN010000299        10569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1581131, whole genome shotgun sequence.
ACCESSION   DASRHN010000299 DASRHN010000000
VERSION     DASRHN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581131"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFB37_12005"
     CDS             complement(<1..194)
                     /locus_tag="VFB37_12005"
                     /inference="COORDINATES: protein motif:HMM:NF042468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase PUA domain-containing
                     protein"
                     /protein_id="HZO23215.1"
                     /translation="MRVTRVYVDSPLLAGRRQRMAGTAANHIARVLRLGIGDALTLFD
                     GRGGEYAGSIDEIGKGFVSV"
     gene            complement(185..1540)
                     /locus_tag="VFB37_12010"
     CDS             complement(185..1540)
                     /locus_tag="VFB37_12010"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HZO23216.1"
                     /translation="MSSANAAYVRRDLAHVWHPCTQMKDHEDLPLIPVRKAHGAWLED
                     FEGRRYLDAVSSWWVNLFGHANPRINAAVRAQLDSLEHVILAGFTHEPVIELSEALVR
                     VLPAGLTRCFYADNGSSAIEVAIKMSFHYWRNVGRPDKRRFITLSNSYHGETLGALSV
                     GNVELYKEIYRPLLMDVITVPSPDCFDREPGESWADHSRRKFAFMESALARHANETAA
                     VILEPLVQCAAGMRMYHPVYLQLLREACDRHGVHLIADEIAVGFGRTGTLFACEQANI
                     RPDLICLSKGLTGGYLPLSAVVTTEEVYAAFYDEYVKLNAFLHSHSFTGNALACSAAL
                     ASLKIFGEDDVLARNRILAARMGNLARELESHAHVAEVRQCGMIVAIELVRDKARREP
                     YAWQERRGLRIYRHALERGVLLRPISNIVYFMPPYVITPEEIDWMVGVAREGIELATC
                     E"
     gene            1680..2273
                     /gene="ampD"
                     /locus_tag="VFB37_12015"
     CDS             1680..2273
                     /gene="ampD"
                     /locus_tag="VFB37_12015"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF008758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HZO23217.1"
                     /translation="MMQPPLQVDPATGLLVGARQVLSPHFDARPTGVLPELIVIHGIS
                     LPPGEFGGPWIDLLFTGKLPADQHPFFRDIASGKVSAHVVIRRDGSITQYVPFGQRAW
                     HAGQSHYRGRTACNDFSIGIELEGADGVTYTDAQYEQLAALVEALLAAFPSLSAEHIV
                     GHDDIAPGRKSDPWSSFDWERWKALLRAHGRLERRRR"
     gene            complement(2326..2997)
                     /locus_tag="VFB37_12020"
     CDS             complement(2326..2997)
                     /locus_tag="VFB37_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZO23218.1"
                     /translation="MQALLDAIQANLADEVTRILEQHPEAAAARSPSGLSALTLAAYL
                     SRPALVELIRRHRGQPDFFEACLLGDEPAVRAGLAGGQDVNACAPDGFTPLGLAVYFG
                     HPAVARLLLQRGAEVNARARNAQQVGPIHAALSRNDLPALELLLLHGADPDLPQQQLF
                     RPLHIAAANGNALAAGLLLLFGAQPAARTEGGKLASELARERGHTVLADKLQKLTATA
                     AGPIG"
     gene            complement(3034..3930)
                     /locus_tag="VFB37_12025"
     CDS             complement(3034..3930)
                     /locus_tag="VFB37_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO23219.1"
                     /translation="MIPTLLGPQFEQLFGANLVFSARAQEYSVRGFPGPFSIKGVARG
                     EAVWRVAGQRFVVDSSSWLIVDRMSPYDLDIHSVEPVETFVVFFADALLADVATTRLA
                     AMERLLDNPAGRSAVELPVSQRLWTGVTEFQQALASLRRASHSSPAVPQGMLDAELRV
                     CLDACADLTARARDEQERIRAARPATRRELYRRVLRGKAHLDETVCSVFDLERAASVA
                     CLAPHHFHRTFRAAFGATPYGYVVRRRIATARRLLAETDSTVEQISDAVGYESLPSFT
                     ARFRRLVGMPPGEFREAIRKHR"
     gene            complement(3927..4490)
                     /locus_tag="VFB37_12030"
     CDS             complement(3927..4490)
                     /locus_tag="VFB37_12030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZO23220.1"
                     /translation="MGNRLSKIYTRTGDDGTTGLGDGSRVPKNGPRVEAFGTVDELNS
                     CIGVLLAVPALPGPVTECLTVIQHELFDMGGELCIPGHQVITAAHVTRIEETLDRFND
                     PLPPLKEFILPGGGPAAAACHVARTIARRAERRVLTLAGSESVSPEVLRYLNRLSDLL
                     FVLARVLARHEQGTEVLWRHDRGTSSS"
     gene            4610..5371
                     /locus_tag="VFB37_12035"
     CDS             4610..5371
                     /locus_tag="VFB37_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZO23221.1"
                     /translation="MPSKELQAAREAAEAAAGIIRRLYRSTLQVRTKPDQSPVTEADI
                     RAEEAIKAVLTERFPSYGFYGEETAGRDLKAPNVWLVDPIDGTKSFLRECPFFSTQIA
                     LMREGQLVLGVSSAPAYDEVAWAEKGGGAYLNGQPIRVSAIGDLAGSIVSSGNLKTLA
                     RSTAWSRFGELIGRVNRIRGYGDFLHYHLLARGALEIVIESDVNILDIAALAVLVREA
                     GGQFTDLCGAPVTLQTTSVLATNGLLHEAVLQALK"
     gene            5480..5806
                     /locus_tag="VFB37_12040"
     CDS             5480..5806
                     /locus_tag="VFB37_12040"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HZO23222.1"
                     /translation="MRNDQATARDLAGKELREALAATEALLAALGDDGGPAVDELRNR
                     LTATIADVRSQIGGTLLSNARDTLSKARDTAVSLDRFVNQRPWTSAVLGCGIGLLIGL
                     VLRSGD"
     gene            complement(5859..6647)
                     /locus_tag="VFB37_12045"
     CDS             complement(5859..6647)
                     /locus_tag="VFB37_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO23223.1"
                     /translation="MDQPLSARGMPLATRSLCVRVGARELVSNLALEFPPGTFVAVLG
                     RNGCGKTLTLHTLAGLREPDSGDVLLDGRTFHEQGRRAAARRLGLLAQDLEEGFVTTV
                     LETVLIGRHPYLAFCQWETSADEQLARKALAAVDMAGFAARRTDTLSGGEQRRVAIAA
                     LLTQDPGVFLLDEPTNHLDPHHQLAVLELFRELARTGRTVIATLHDPTLAARFADRAL
                     LLHGDGRWSEGPVSEVITQGSLSELYLAPMVELENAGRRIFVTR"
     gene            complement(6632..7405)
                     /locus_tag="VFB37_12050"
     CDS             complement(6632..7405)
                     /locus_tag="VFB37_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23224.1"
                     /translation="MWRIIRTALLLLVLIWAAAHTWLDRFESTRWHDPLWVGIFPVNA
                     DGSTISQQYIDALTSREYADIEAFFVREAQRYGKAVTPPVHMVLYPQSRQTPPVLERN
                     AGPVSTAWWSLKLRWYAFRAADTGGRPPPRIRLFVLYHDPTTLQTVADSHGLQKGLLG
                     VVHVFALRPMAGSNSIVIAHELLHTLGATDKYDLRTGMPDYPSGFAEPDRRPLYPQPL
                     AEIMAGRQALSASRSEMPGSLRDVVVGPATAREIRWTNP"
     gene            complement(7412..8107)
                     /gene="pdxH"
                     /locus_tag="VFB37_12055"
     CDS             complement(7412..8107)
                     /gene="pdxH"
                     /locus_tag="VFB37_12055"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZO23225.1"
                     /translation="MSRHTELLRDPLPTEPLLVVNQWLAESWKLRQQPNPNSMVIATV
                     DPQGRPSARVVLCKEIVPQPGYLVFYTNYLSRKGLDLARTPRAAVVMHWDTLHRQVRI
                     EGPVAQAPAADSDAYFASRPWQSRLGAWASAQSQPIGARKDLQAAVAQAAKRFGVPVP
                     GGGSEDSQAATDYPIPRPPHWGGYHVWAEAVELWVEGEARIHDRARWTRALQKRADGL
                     FETGPWSSTRLQP"
     gene            8238..9362
                     /locus_tag="VFB37_12060"
     CDS             8238..9362
                     /locus_tag="VFB37_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZO23226.1"
                     /translation="MTQDDVTLSTLRERLTAIDRQMISLVAERKKLSGEVARVKRATG
                     FPTRDFERERDVILGVRKMAAELGVSPALAEDLLHLLIRSSLTTQEQASVVARGTGSG
                     KRVLVIGGAGKMGRWFAEFLSSQGFQVEIADPGAAPAGLSGVEDWRKTELTHDYIVLA
                     TPLGVTDAILRELALRRPPGIIFDIGSLKSPLRAGLMALKSHGCKVTSLHPMFGPDTE
                     LLSGRHVVFVDLGCAEALSRARELFAPTMAEQVVMSLDEHDRLIAYVLGLSHALNIAF
                     FTALAGSGEAAPKLARMSSTTFDAQLDVASRVAQESPELYYEIQSLNDYGAESLEALS
                     QAVERVRTAVVAQDHDAFVSIMQKGRDYLEDRRSVTARRA"
     gene            9440..10567
                     /locus_tag="VFB37_12065"
     CDS             9440..10567
                     /locus_tag="VFB37_12065"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZO23227.1"
                     /translation="MGRRSQDEGTRPAVREKLDVEVRALQERLTELTEEVSRNDALLR
                     KTQERELELIRATSLPQLLDLMIHGLRTSYQLDVVTLALHDPNHEVRHLLSGDGFRVE
                     DLPEVQFADQVVELAPQLASLDRPWLGPFRTADHALLLGGAGQRGSLALIPLRRNESL
                     DGALVFFSVDKRRFTSELASDFLAHLGVVGAICLENAINRARLVRSGLTDFLTGFHNR
                     RYLQARLGEELARAQREQHAVACLMIDVDHFKRINDRHGHLAGDAVLREVAHRISDHM
                     RLSDTGARFGGDEFAIVLPHGTLKDAEKVATRVLDAVRSQPIDIGGRQGETVSLSIGV
                     AAASALPKGTRDYSQLAERLLAEADAALYRAKSEGRNRVCI"
BASE COUNT         1863 a         3402 c         3498 g         1806 t
ORIGIN      
        1 tccacggata cgaagccctt gccgatctca tcgatgctgc ctgcgtattc gccgcctcgt
       61 ccgtcgaaga gtgtcagcgc atctccgata cccaaccgca acacccgggc gatgtgattc
      121 gctgcggtgc cggccatcct ctgtcgccgc cccgccagca gcggcgagtc gacatagacg
      181 cgcgtcactc gcatgtcgcg agctcgattc cctctcgagc cacgccgacc atccagtcga
      241 tctcttcggg cgtgatcacg tatggaggca tgaagtagac gatgttggag atgggacgca
      301 gcagcactcc gcgctcgagc gcgtgacggt agattcgcag cccgcgccgc tcctgccagg
      361 cataaggctc acgccgggcc ttgtcccgca ccagctcgat ggccacgatc attccacact
      421 gcctgacctc cgccacgtga gcatggctct cgagctcgcg ggcgaggttg cccatgcggg
      481 cggcgagaat gcggttgcgc gcgagtacat catcctcgcc gaagatcttg agactggcca
      541 gcgccgccga acacgccaac gcattgcccg tgaagctatg cgaatgcagg aaggcgttca
      601 gcttgacgta ctcgtcgtag aaagctgcat agacctcctc ggtagtgacg acggccgaga
      661 gaggcaggta cccgccagtc aggcctttcg acaggcagat gaggtcggga cggatgttgg
      721 cctgttcgca ggcgaacagg gtaccggtgc ggccgaatcc gaccgcgatc tcatcggcga
      781 tgaggtgaac gccgtgacgg tcgcacgctt cccgcagcag ctgcagatag accggatggt
      841 acatgcgcat gcccgcggcg cactgcacga ggggctcgag gatgaccgct gcggtctcgt
      901 tcgcatgccg ggccagtgcg ctctccatga aggcgaactt gcgccgcgag tgatcggccc
      961 agctctcgcc cggctcgcga tcgaagcaat cgggtgaggg aacggtgatc acatccatga
     1021 gcagcggacg atagatctcc ttgtacagct cgacgttgcc gacggacagg gcaccgaggg
     1081 tctcgccgtg atagctgttg gagagcgtga tgaagcgccg cttgtccggg cgacccacat
     1141 tgcgccagta gtggaagctc atcttgatcg ccacctcgat cgccgatgag ccattgtccg
     1201 catagaagca gcgtgtcagt ccggctggaa gcacccggac gagcgcctcc gagagctcga
     1261 tgaccggctc atgcgtgaag cccgcgagaa tcacgtgctc gagactgtcc agctgggcac
     1321 ggacggccgc gttgatgcgc ggattggcat gcccgaagag gttgacccac caggagctga
     1381 cggcatcgag atatcggcgg ccctcgaaat cctcgagcca ggcgccgtgg gccttgcgca
     1441 cgggaatcag cggcagatcc tcgtggtcct tcatctgggt gcagggatgc cagacgtggg
     1501 ccagatccct gcggacgtac gcggcgtttg cgctgctcat gcaaagattg tggcacgagc
     1561 gcgcgcacag ttggctgcac gacgcgtgtt acgatcaacg ccgatgaacg gcggctgccg
     1621 gaatgcggcg ctgtaagtca ttgattggaa gaagggtatc gctcgtggca tgatcccaga
     1681 tgatgcagcc gcccctgcaa gtcgatcccg cgacgggact cctggtgggt gcccgccagg
     1741 tcctgtcgcc ccatttcgac gcccggccca ccggtgtgct tccggagctg atcgtcattc
     1801 acgggatcag cctgccgccg ggtgagttcg gcggaccgtg gatcgatctg ctgttcactg
     1861 gaaagctgcc cgcggatcag cacccttttt tccgcgacat cgcttccggg aaggtctcgg
     1921 cgcacgtcgt catccggcgc gatggctcga tcacgcagta cgtgccgttc ggtcagcgag
     1981 cctggcacgc cggccagtcg cactatcgcg gccgtacggc gtgcaatgac ttttccatcg
     2041 gcatcgagct cgagggcgcc gacggcgtga cctacacgga tgcgcaatac gagcagctcg
     2101 cagccctggt ggaagctctg ctcgctgcct tcccctccct gtcggccgag cacatcgtcg
     2161 gtcacgatga catcgctcca gggcgcaagt cggatccgtg gtcgtccttc gactgggagc
     2221 gttggaaggc cctgctgcgc gcgcacggtc gcctggagcg gcggcggcgc taagcagggc
     2281 gacgctcggc tcgcgtggac gctttcacga gcgcccggcg ctggcctagc cgattggccc
     2341 ggcagcggtt gcggtcagct tctgcagctt gtcggccagc accgtgtgtc cgcgttcgcg
     2401 agccagctct gatgcaagct tgccgccctc ggttctcgcg gcgggctgcg caccaaacaa
     2461 cagcaacaga cccgccgcca gcgcgttgcc gttcgcggcc gcgatgtgca gcggccggaa
     2521 aagctgctgc tgcggcaagt ccgggtcggc gccgtggagc agcagcagct ccagcgcagg
     2581 caggtcgttg cgactgagcg ccgcgtggat gggaccgacc tgctgggcat ttcgtgcccg
     2641 tgcattcacc tctgcgcccc tctgaagcag gagtctggcc acggcggggt ggccgaaata
     2701 cacggccaga cccagaggtg tgaatccgtc aggtgcgcat gcgttgacgt cctggccccc
     2761 cgccagacct gcgcgcactg ccggctcatc gcccagcaga caggcctcga aaaagtccgg
     2821 ctgcccacga tgccgacgga tcaattcaac gagcgccggc cgcgacagat acgccgccag
     2881 agtcagggca gacaggccgc tcggggaacg cgcggcggcc gcctccgggt gctgctcgag
     2941 gatgcgtgtg acctcatccg ccagattggc ctggatggca tcgagaaggg cttgcatgct
     3001 gctggatgct agcggctcgg cggctcggag tgtttatcgg tgcttgcgaa ttgcttcgcg
     3061 gaactcgccc ggtggcatcc cgacgagacg ccggaatcgg gccgtgaatg atggcaggct
     3121 ctcatagccg accgcatcgc tgatctgttc cacggtgctg tccgtttcgg cgaggagccg
     3181 gcgggccgtg gcgattcgtc gccggaccac gtagccgtac ggtgtggctc cgaatgcggc
     3241 tcggaacgtg cgatggaagt gatgcggagc gaggcaggcc acgctggctg cacgctccag
     3301 atcgaacacg ctgcagacag tctcgtccag atgcgccttg ccgcgcagaa cacgccggta
     3361 cagctcacga cgggtcgcgg ggcgggccgc ccggatacgc tcctgctcat cgcgcgcacg
     3421 cgccgtgagg tcggcgcacg cgtcgaggca aactcgcagc tcagcatcga gcatgccctg
     3481 cgggactgca ggcgatgaat gactggcgcg tcgcaaggac gccagcgcct gctggaactc
     3541 ggtaacaccg gtccacagcc gttgcgagac tggaagctcg actgcactgc gtccggcggg
     3601 gttatcgagc agccgctcca tggcggccag acgggtagtc gccacatccg ccagtaaggc
     3661 atctgcgaag aacacgacga aggtctccac tggctcgaca gagtggatat ccaggtcgta
     3721 gggcgacatc cggtcgacga tgagccagct gctcgagtcc actacgaacc gctggcccgc
     3781 gacgcgccag accgcttcgc cccgcgcgac accctttatc gagaaggggc ctggaaagcc
     3841 ccggaccgaa tattcctgcg cccgggcgct gaagacgaga ttggcgccaa agagctgctc
     3901 gaactgcgga ccgagaaggg tcggaatcac gagctggagg ttccccgatc gtgccgccag
     3961 agcacttccg tgccctgctc gtgacgcgcc aggacgcgag cgagcacgaa cagcagatcc
     4021 gaaagacggt tgaggtatcg caacacttcc ggactgacgg actcgctccc agccagtgtc
     4081 agcacgcggc gctcggcacg gcgtgcaatc gtgcgcgcga catggcaggc ggctgcggcg
     4141 gggccgccgc ctggaagaat gaattccttc aggggcggca acggatcgtt gaaacgatcg
     4201 agcgtctcct cgatacgcgt gacatgcgcc gcggtgatga cctgatggcc cggaatgcac
     4261 agctcgccac ccatgtcgaa cagctcgtgt tggattactg tcagacactc ggtcaccgga
     4321 cccggcagag cgggcaccgc cagcagcacg ccgatgcagc tgttgagctc atcaacggtc
     4381 ccgaatgcct cgacccgcgg cccattcttc gggacgcggc ttccatctcc gagtccggtc
     4441 gtcccgtcat cgcctgtgcg cgtgtagatc ttgctcagcc ggttgcccat cgacaggccc
     4501 tcgcgcggtc gctcactcag tacctcggga attctgccag aacagtcgcg ttgacaggtc
     4561 cttggcgcct cggcgaggct gcgctgatcc ggcctgccga aagtccagca tgccctccaa
     4621 agaactccag gctgcccgcg aggcggccga agccgccgcc gggatcatcc ggcggctgta
     4681 ccgcagcact ctgcaggtga ggaccaagcc agatcaatca cctgttacgg aggcggacat
     4741 acgggcggaa gaggcgatca aggcagtgct gaccgagcgc ttccccagct atggcttcta
     4801 tggagaagag accgccggtc gcgacctgaa ggccccaaac gtgtggctgg tggacccgat
     4861 cgatggcacg aagtcgttcc tgcgggagtg cccgttcttc tccacgcaga tcgcgctcat
     4921 gcgagaggga cagctcgtgc tgggggtctc cagtgcgccg gcgtacgacg aagtcgcgtg
     4981 ggccgagaag ggcgggggag cctacctgaa cgggcagccg atccgggtca gcgccatcgg
     5041 ggatcttgcc ggcagcatcg tctcgagcgg caacctcaaa accctggcgc gctcaaccgc
     5101 ttggagccga ttcggcgagc tcattggccg tgtgaatcgc atccgcggtt acggggactt
     5161 cctgcactac cacctgctgg cgcgtggcgc cttggagatc gtgatcgagt ccgacgtgaa
     5221 catcctcgac atcgcggcgc tcgctgtcct ggtgcgcgag gcaggcgggc agttcacaga
     5281 cctgtgcggc gcgcccgtga ctctgcagac caccagcgta cttgcgacca acggcctttt
     5341 gcacgaggcc gtgctacagg ccctgaagtg agcgccacct ggtgcgatgc aaccgccgcc
     5401 gccggacggg aggtgctcgc ggaactccgg cccggagccg gcgttgatgc cccagcttgg
     5461 gacgaagcag gttcgagtca tgagaaacga tcaggctacg gccagagatc tggccggaaa
     5521 ggagctgcgc gaggcgctcg ccgccacgga ggctctcctg gcagctttgg gggacgatgg
     5581 tggacccgcc gtcgacgaat tgcggaaccg gctgaccgct accattgccg atgtccgcag
     5641 ccagataggg ggtacgcttc tgtcaaatgc gcgcgacacc ctgtcgaagg cccgcgacac
     5701 ggcggtctcc ctggatcgct tcgtgaacca gcgaccctgg acatcggcgg tactcggttg
     5761 cggaatcgga ctgctgattg ggctggtgct gagaagcggc gactgatttc gctacgcggc
     5821 ggccgctggc gtgccggctt tccgagccgg gcgggtccct aacgggtgac gaagattcgc
     5881 cggccggcgt tctcgagctc caccatgggc gccagataca gctcgctcag cgagccctgc
     5941 gtgattactt cgctcacggg gccctcgctc cagcggccat ctccatgcag gagcagcgcg
     6001 cgatcggcga agcgcgcggc gagggtcggg tcgtgcaggg ttgcgatcac ggtgcgcccg
     6061 gtgcgcgcga gctcgcgaaa cagctcgagc accgcgagct gatgatgcgg atcgagatgg
     6121 ttcgtcggct catccagcag gaataccccc ggatcctgcg tcagtaatgc ggcgatggcc
     6181 acgcgccgct gctcgccacc cgagagcgta tcggtgcggc gggccgcgaa gccggccatg
     6241 tccacggcgg ccagcgcctt tctggccagc tgctcgtccg ccgaagtttc ccactggcag
     6301 aatgcaagat aaggatgacg cccgatcagc acggtctcga gaacggtggt cacgaaaccc
     6361 tcttccagat cctgggcgag cagccccagc cgtcgggccg cagctcgccg accctgttcg
     6421 tggaacgtgc gtccgtcgag gagcacgtcg cccgagtcgg gctcccgcag ccccgctagc
     6481 gtatgcaagg tgagcgtctt cccgcagccg ttgcggccaa gcacggccac gaacgtcccg
     6541 ggcgggaatt ccagcgccag attgctcacc agctcgcgcg cgccgacgcg aacgcagagg
     6601 cttctggtgg cgagcggcat cccgcgagcg ctcaggggtt ggtccatcga atctcccgcg
     6661 cggtggccgg gccgacgacc acatcccgaa gcgacccggg catctcggac cggctcgccg
     6721 agagggcttg ccggccggcc atgatttcag ccaagggctg gggataaagc ggcctgcgat
     6781 cgggttcggc gaatccgctc gggtaatcag gcatgccggt gcggaggtca tacttgtccg
     6841 tggcgccgag cgtgtgcaga agctcgtgtg cgatcacgat gctgttgctt cccgccatgg
     6901 gtcggagcgc gaacacgtga accacgccga gcagtccttt ttgcagacca tgcgagtctg
     6961 ccaccgtctg cagagttgtc gggtcgtgat agagcacgaa gagccggatc cgcggcggcg
     7021 gccgcccccc ggtgtcggcc gcccggaagg cgtaccagcg caatttcagg ctccaccagg
     7081 ccgttgacac tgggcctgca ttcctctcca gaacgggcgg agtctgtctg gattgcgggt
     7141 acaggaccat gtgcacgggc ggtgtcacag ccttgccgta tcgctgcgcc tcacgcacga
     7201 agaaggcttc aatatcagca tattcgcggg acgtcagagc gtcgatgtac tgctgcgaga
     7261 tcgtgctgcc gtcggcattc acaggaaata ttccgaccca gagcggatcg tgccagcggg
     7321 tgctctcgaa tctgtccagc caggtgtgag cggccgccca gatcaacacc agcagcagca
     7381 acgctgtgcg aatgatccgc cacacggtgc gctaaggctg caggcgggta ctgctccagg
     7441 gacccgtctc aaacaatcca tcggcacgct tctgcaacgc gcgggtccat ctggcgcggt
     7501 cgtgaatgcg cgcttcgcct tccacccaca gctcgaccgc ctcggcccaa acatggtatc
     7561 cgccccagtg cggcggccgg gggatagggt agtccgtcgc cgcctgagaa tcctccgagc
     7621 ccccgccagg caccggcacc ccgaagcgct tagcggcttg cgcaacggcg gcctggaggt
     7681 ccttgcgcgc accaatgggc tgactctggg cactggccca ggcgcccagc cggctttgcc
     7741 atggtcgcga ggcaaagtac gcatcactat cggcggcggg ggcctgagcc accgggccct
     7801 cgatgcgcac ctggcggtgc agagtgtccc agtgcatcac gactgcagcc cggggagttc
     7861 gcgcgagatc caagcccttt cgggacagat aattcgtata gaacaccagg tagcccggtt
     7921 gcggaacgat ctccttgcag agcacgacac gcgcggaggg tctgccttgc gggtccaccg
     7981 tcgcgatcac catcgaattg ggattgggct gctgtcgaag tttccagctc tcggccagcc
     8041 actgattgac gacgaggaga ggctcggtcg gcagcggatc acgcagcagc tcggtatggc
     8101 ggctcattgc gcaatcgtaa accagtcgat aaaaagggtg tcacttcatc cgccagagcg
     8161 gtgggggccc ggcctggcgg tcctgcggga ccctccacca cacgggcgtt tcagtctacc
     8221 gaggagccga ggctgccatg acgcaggacg atgtgacgct gagtacgctg cgcgagcgcc
     8281 tgaccgccat tgatcgacag atgatttcgc tggttgccga gcgcaaaaag ctcagtggcg
     8341 aggtcgcccg agtgaaacgc gccaccggct ttcccacgcg cgatttcgag cgcgagcgcg
     8401 atgtcattct gggtgtcaga aagatggctg cggagctggg tgtctccccg gctctggcgg
     8461 aggacctcct gcatctgctg atccgttcct cgctcaccac gcaggagcag gccagtgtcg
     8521 tggcccgcgg gacaggctcg gggaagcggg ttctggtgat cggcggtgct ggcaagatgg
     8581 gacgctggtt cgccgagttc ctcagctcac agggctttca ggtcgagatt gccgacccgg
     8641 gtgcggctcc ggccggactg tccggagtgg aggactggcg caagaccgag ctcactcatg
     8701 attacatcgt cctggcgacg ccactcgggg tgaccgatgc catcctgcgc gagctggcgc
     8761 tgcgtcgtcc gcccggtatc atcttcgaca tcggctcgct caaatcgccg ttgcgcgccg
     8821 ggctcatggc gctcaagtcg cacggttgca aagtgacatc gttgcatccg atgttcggtc
     8881 ccgacaccga gctgctgtcc ggccgtcacg tcgttttcgt ggatctgggc tgcgccgagg
     8941 cgctcagccg ggcgcgtgag ttgttcgcgc ccacgatggc ggagcaggtg gtcatgagcc
     9001 tcgacgagca tgaccggctg atcgcctacg tactcgggct ttcgcacgca ctcaacattg
     9061 cattcttcac ggcgctggcc gggagtggcg aggccgcgcc gaagcttgcg cgcatgtcga
     9121 gtacgacgtt cgacgcacag ctcgatgtcg caagccgcgt ggcgcaggaa agtccggagc
     9181 tgtattacga gatccagagc ctcaacgatt acggggcaga atctctcgag gcgctctctc
     9241 aagcggtcga acgcgtccgt acagccgtcg tggcgcagga tcacgatgct ttcgtatcca
     9301 tcatgcagaa gggccgcgat tatctcgagg atcgtcgcag cgtgacggcg cggcgggcct
     9361 agcgcttcgt ctgctggcag cagcggccgg ggcggcgcga gcattcctga cggggcgaat
     9421 atccatgacg gatcagaggt tgggtaggag atcgcaggat gagggcaccc gcccggcggt
     9481 tcgcgaaaag ctcgatgtgg aagtgcgggc gctgcaggag cgcctgaccg agttgaccga
     9541 agaggtcagc cgaaacgatg cgctgttacg caagacccag gaacgcgagc tcgagctgat
     9601 acgtgcgacc tctttgccgc agcttctcga tctcatgatt cacgggctgc gcacctccta
     9661 tcagctcgat gtcgtcaccc tcgccctgca cgatcccaac catgaggtga ggcatctgct
     9721 ctccggcgac ggcttccgcg tggaggatct gccagaagtc cagttcgccg atcaggtcgt
     9781 cgagctggca ccgcaactgg cgagcctcga tcggccgtgg ctgggaccgt tccggacggc
     9841 ggatcacgcc ttgctgctgg gcggcgcggg acagcgaggc agtctggcgc tcatcccact
     9901 gcgtcgcaat gagtcacttg acggcgccct ggtattcttc agcgtcgaca aacggcgctt
     9961 cacatccgag ctggcgagtg acttcctcgc tcatctgggg gtggtcggcg cgatctgtct
    10021 cgagaatgcc atcaatcgcg cgcggctcgt gcgcagcgga ttgaccgatt tcctcaccgg
    10081 ctttcacaac cgacgctacc tccaggcccg cctgggcgaa gagctggcac gcgcgcaacg
    10141 tgagcagcat gccgtggcct gcctgatgat cgatgtcgat catttcaagc gcatcaacga
    10201 ccgccacgga catctcgccg gtgacgcggt tctgcgcgaa gtcgcacacc gcatcagcga
    10261 ccacatgcgc ctgagcgaca cgggggcccg cttcggtgga gacgagttcg cgatcgtgct
    10321 tccccacggg acactgaagg acgcggagaa ggtcgccacc cgcgtgctcg atgcagtgcg
    10381 cagccagcca atcgacattg gaggtcggca gggagagacg gtgtcgctat ctatcggagt
    10441 cgccgcagca tcagctctcc ctaaaggtac ccgcgattac tcgcagctcg ccgagcgctt
    10501 gctcgcggag gctgacgcgg cgctttaccg ggccaagagc gagggccgta accgggtgtg
    10561 tatctgata
//
LOCUS       DASRHN010000300         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1920182, whole genome shotgun sequence.
ACCESSION   DASRHN010000300 DASRHN010000000
VERSION     DASRHN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920182"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFB37_12070"
     CDS             complement(<1..598)
                     /locus_tag="VFB37_12070"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HZO23228.1"
                     /translation="MVPVDWPSGAHVVAMMQRIWALLDWCFFGPASTRGDLLALLIRT
                     LRYPYAVVRDLWRGEINLRAMGLVYTTLLSIIPLVAFSFAILKVFGAHRDLEPIVYEF
                     FRPLGGEPATQMTGRVMQFADRVSSGVVGSLGFALLAWTLLGTIKKVEDSFNFLWRVE
                     QPRSFARRIAEYLTLLVVGPVLLVAFIGLSHATIASTPV"
     gene            605..1411
                     /locus_tag="VFB37_12075"
     CDS             605..1411
                     /locus_tag="VFB37_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO23229.1"
                     /translation="MIPEPLFPQRIVCLTEETTETLYLLEEDWRIVGISGFTVRPPRA
                     RREKPRISAFTSAKIDRILELRPDLVLGFSDLQADIAAELVRAGIEVHVFNHRSVGEI
                     LRMIGTLGGMIGCQPKARLLIERLSAGLDEVRDRAARLSRRPRVYFEEWDEPPISCIR
                     WVSELIGLAGGDECFPELARESLGRNRILADPLEVPRRAPDIILGSWCGKKFRPEQVA
                     TRPGWEAIPAIRQGFVREIKSAIILQPGPAALTDGVRAIQGVIEEWAARQ"
     gene            complement(1426..>2189)
                     /gene="djlA"
                     /locus_tag="VFB37_12080"
     CDS             complement(1426..>2189)
                     /gene="djlA"
                     /locus_tag="VFB37_12080"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HZO23230.1"
                     /translation="GLLAGGPIGAAVGVLLGHQFDEYSGDGADEPRLRNDSAAVGDQF
                     FRAAFRVMGYVAKSDGRVSESEIAAARAVMSDLRLTQPQVRMAIDYFTDGKQPDFNLN
                     AELASLAASCRGRPGLLRTFLEIQVRAALAGNNLEGPVRQLLGYMANRLGVSMVELAQ
                     IEAVLRIRSGTFRQQTTRNTGNEQLQQAYSVLECSPSTSDGELVKAYRRQLSRHHPDK
                     LKANGLPESMIEHAKQRTQQIIEAYELIRERRGLR"
BASE COUNT          401 a          674 c          734 g          380 t
ORIGIN      
        1 gcaccggggt gctcgcaatg gttgcgtgag agagcccgat gaaggcaacg aggagcacag
       61 gtccgacgac cagtaatgtc aggtattcgg ctatgcgccg ggcgaagctg cgtggttgct
      121 cgacacgcca caggaagttg aagctgtcct ccaccttctt gatcgtaccg agcagtgtcc
      181 aggccagcag ggcaaagccg agtgagccga caacaccgct gctgacacgg tcggcgaatt
      241 gcatgacccg ccccgtcatc tgcgtggctg gctcaccgcc aagcggacgg aagaactcgt
      301 agacgatggg ttccagatcg cgatgggcac caaagacctt cagaatcgcg aaactgaagg
      361 ccacgagagg aatgatcgac aacagcgtgg tgtagaccag gcccatggcg cgcagattga
      421 tctctccccg ccacaggtcg cggaccacgg cgtagggata gcgcaaggtc cgaatcagca
      481 gcgccaggag gtctccgcgc gtcgaagcgg gcccgaaaaa gcaccagtcg agtaaagccc
      541 agattcgctg catcatcgca accacgtgcg caccggatgg ccagtctaca ggtaccatcg
      601 ccggatgatc ccggagcccc tttttccgca acggattgtc tgcctgaccg aggaaacgac
      661 cgagacgctg tacctgctgg aggaggactg gcggatcgtg ggtatttcag gctttacagt
      721 gcggccgccg cgcgcgcgcc gggaaaagcc ccgcatcagc gccttcacca gcgcgaaaat
      781 agacaggatt ctggagctgc ggccggacct ggttctgggg ttctccgatc tgcaggctga
      841 catcgctgcc gagctggtgc gtgcgggaat cgaggtgcac gtattcaatc atcgcagcgt
      901 cggggagatt ctgcggatga tcggcaccct cggcgggatg atcggctgcc aacccaaggc
      961 gcggctgctg atcgagcgcc tgtcagcggg cctcgatgag gtgcgagatc gggctgcacg
     1021 cctgtcccgc cgaccccggg tgtacttcga ggagtgggat gagccaccga tctcctgtat
     1081 caggtgggtc tcggagctga tcggactggc cggtggcgat gagtgctttc cagagctcgc
     1141 tcgcgagtct ctggggcgca accgcattct ggccgatccg ctggaggtgc cgcgacgcgc
     1201 gccggacatc atcctgggtt cctggtgtgg caagaaattc cggccggagc aggtcgccac
     1261 gcggccaggg tgggaggcta ttccggcaat ccgccaggga ttcgtgcgcg agatcaagtc
     1321 ggccatcatc ctgcagcctg gaccggcggc cctgacggat ggcgtgcgtg ccatccaagg
     1381 tgtcatcgag gagtgggccg cgcggcagtg aggctcgcgc ccccgtcaac gcagtccgcg
     1441 gcgctcgcgg atcagctcgt aggcttcgat gatctgttga gtacgctgct tggcgtgctc
     1501 gatcatcgac tccggcaggc cgttggcctt gagtttatcc gggtgatgac ggctcagctg
     1561 ccggcggtag gccttgacca gctcgccatc gctggtggag ggcgagcact cgagcacact
     1621 gtaggcctgt tgcagctgct cgttcccggt attgcgtgtt gtctgctggc ggaaggtgcc
     1681 gctgcggatg cgcagcacgg cttcgatctg cgccagctcc accatcgaga caccgagccg
     1741 gttggccatg tagcccagca actgtcgcac agggccttcc aggttgttac ccgccagcgc
     1801 ggcgcgcacc tgaatctcca ggaacgtgcg cagcaggcct ggccggccgc ggcagctggc
     1861 cgcgagcgag gcgagctccg cgttgaggtt gaagtcaggt tgtttgccat cggtgaaata
     1921 atcgatggcc attctcacct gcggctgcgt gagccgcagg tcggacatga ccgcccgggc
     1981 ggcggcgatc tcgctctccg atacccggcc gtccgatttg gcgacgtacc ccatgactcg
     2041 gaaagcggcc cggaagaact gatcgccgac ggcggctgag tcgttgcgca ggcgcggctc
     2101 atccgcgccg tcgccggaat attcatcgaa ctggtggccc agcagcacgc caacggcagc
     2161 tccgatagga cctcccgcga gcagaccga
//
LOCUS       DASRHN010000301         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2083458, whole genome shotgun sequence.
ACCESSION   DASRHN010000301 DASRHN010000000
VERSION     DASRHN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083458"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1129)
                     /gene="aroC"
                     /locus_tag="VFB37_12085"
     CDS             complement(32..1129)
                     /gene="aroC"
                     /locus_tag="VFB37_12085"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZO23231.1"
                     /translation="MSGNTIGRLFTVTTFGESHGPAMGCIVDGCPPGISLSEAEVQQD
                     VDRRRTGTSPFVSQRREGDQVRILSGVFEGRTTGTPIGLLIENTDARARDYEKIKDRF
                     RPGHADYTYQQKYGSRDYRGGGRSSARETVMRVAAGAIARKYLGERLGVRIYGYLSQI
                     GSLQIDAVDPAFAYQNPFFCPDPTRVVQIEELMWNTRAAGDSVGARVTVVAAGVPPGL
                     GEPVFDRLDADIAHALMGINAVKAVEIGAGAAAAAQRGSEHRDELTPAGFTSNHAGGI
                     LGGISSGQDIVAHMTLKPTSSIQVPGRTIDVKGEPVEIVTTGRHDPCVGLRATPIAEA
                     MLAIVLVDHYLRHRAQNGDVRSPTPDIARGK"
     gene            complement(1147..2019)
                     /gene="prmB"
                     /locus_tag="VFB37_12090"
     CDS             complement(1147..2019)
                     /gene="prmB"
                     /locus_tag="VFB37_12090"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZO23232.1"
                     /translation="MVLVRDLIASSARRLQRAGVFFGHGTDNARDEAATLVLHALGHS
                     PHGSRSVLSRRVSEAGRRRAAALITVRIKKRIPAAYLTGETWFAGLSMRVDPRVLIPR
                     SALAELIEHRFAPWIDPRRVQRVLDVGTGSGCIAIACAKAFPPARVDALDISKAALEV
                     ARVNVRRHRLSRRVRLLESDHFGALHRESYDIIVSNPPYVGARELRTLPSEYRHEPRI
                     ALAAGASGLDSIRILLQQASRFLRAAGLLVVEVGNTEVAVRRAFGNLPFTWLEFERGG
                     GGVFLLTAEQLRTR"
     gene            2045..2665
                     /locus_tag="VFB37_12095"
     CDS             2045..2665
                     /locus_tag="VFB37_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZO23233.1"
                     /translation="MTQRSSWILLILAGLLAALAGFGLSRKLDSSGPQLASGTWLPAP
                     RAVGDFHLTDHLGQPFTAQNLQGKVSLVFFGFTHCPDVCPTTLAKLAQVRKVAAVPAL
                     QVLFVTVDPERDTPTAMGLYVHAFDPEFIGLTGDTQEIQRLSAKFGIAATRVELPGGD
                     YTMDHSAAVFLLDRQGRIAAIFTPPFDIQRFALDLHQSLPYLGSRS"
     gene            2676..3566
                     /gene="asd"
                     /locus_tag="VFB37_12100"
     CDS             2676..3566
                     /gene="asd"
                     /locus_tag="VFB37_12100"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HZO23234.1"
                     /translation="MSEEAPAGIRERLFVALQHVIPQHFLSHLVYRLARSRSPGLKNR
                     LIQAFVRSYRPEMREAAEPDPLRYASFNDFFTRSLRAGSRPVDPAARSVVSPVDGLIS
                     QIGHLEESRIVQAKGHDYSVAELLGLEAGSSWPQRFAGGSFTTLYLAPFDYHRVHMPL
                     EGTLRAAWYVPGRLFSVNAVTAASVPRLFARNERIICLFEGRTAPFAVVLVGALFVGS
                     MSTVWHGEITPRSPPQRLDLPLQSTHAPLHLSKGAEMGRFNMGSTVILLLPPGASTWL
                     PEWRSGERVLVGQVLAYLGA"
     gene            3563..>3890
                     /locus_tag="VFB37_12105"
     CDS             3563..>3890
                     /locus_tag="VFB37_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZO23235.1"
                     /translation="MKTDSALSWQPTTAPLTLKRRAALLAQTRKFFADRGVLEVDTPM
                     VVHAAVTDVHIHSARVELEQGGAADRPERSFFLHTSPEYAMKRLLAAGSGDIYQICHV
                     VRALERS"
BASE COUNT          641 a         1326 c         1233 g          690 t
ORIGIN      
        1 ccgccaccga gaatgatttc gcttcgctca cctacttgcc tcgcgcgatg tcgggagtcg
       61 ggctgcgcac gtcgccgttc tgggcgcgat ggcgcaggta atgatccacc agcacgatgg
      121 ccagcatcgc ctcggcgatc ggcgtggccc gcagcccgac acaggggtca tgccggccgg
      181 tcgtcacgat ttcgaccggc tcacccttga cgtcgatcgt cctgccgggc acctggatgc
      241 tcgaggtcgg cttgagcgtc atgtgggcca cgatgtcctg ccccgaagag attccgccaa
      301 ggatgccacc agcgtgattg ctggtgaaac ccgcgggcgt gagttcgtcc cggtgctcgc
      361 tgccacgctg cgccgccgct gctgcgccgg cgccgatctc gacggccttg accgcgttga
      421 tacccatcaa cgcatgggcg atgtccgcat ccagcctgtc gaacacgggc tcgccgaggc
      481 ccggtggcac cccggcggcg accaccgtga cgcgcgcgcc gacggagtcg ccggcggcgc
      541 gggtgttcca catcaactcc tcgatctgca ccacgcgcgt cgggtcgggg caaaagaacg
      601 gattctgata cgcaaaggcg ggatcgacag catcgatctg caaggagccg atctggctga
      661 gatagccgta gatccgtacc cccagccgct cgccgagata tttgcgtgcg atggcgcccg
      721 cggccacgcg catcacggtc tcgcgcgccg aagagcggcc gccgccgcga tagtcgcggg
      781 aaccatattt ctgttgatag gtgtaatcgg catgcccggg acgaaagcgg tccttgatct
      841 tctcgtaatc ccgcgcgcgc gcatcggtgt tctcgatgag cagcccgatc ggggtgcccg
      901 tggtgcggcc ctcgaacacg cccgaaagga tgcgcacctg gtcgccttcg cggcgctgac
      961 tcacgaacgg cgaggttccg gtacggcgcc ggtcgacgtc ctgctgcacc tcggcctcac
     1021 tcagcgaaat accgggcggg cagccatcca cgatacagcc catcgccggc ccgtgacttt
     1081 cgccgaaggt ggtgaccgtg aagagtctgc cgatcgtgtt gccagacatg ctgggaggtt
     1141 cgtatctcaa cgggtccgca attgctcggc ggtcagcaga aacaccccgc caccgccgcg
     1201 ctcgaactcg agccacgtga agggcaggtt gccgaatgca cgccgcaccg ccacttccgt
     1261 gttgcccacc tccacgacca gcaggcctgc ggcccgcagg aagcgcgagg cctgctgcag
     1321 gaggatccgg atggagtcga gtccgcttgc gccggcggcc agcgcaatgc gcggctcatg
     1381 gcgatactcg gacggtaagg tgcgcagctc gcgggccccc acgtagggcg gattcgacac
     1441 gatgatatcg tagctttccc gatgcagcgc accgaaatga tcggattcga gaaggcggac
     1501 acgccggctg agccggtgcc gccggacatt tacacgcgcg acctccaagg ccgcctttga
     1561 gatatccaac gcgtccaccc gcgcgggcgg aaatgccttg gcacaggcga tggcgatgca
     1621 tcccgagccg gtgcctacat cgagcactcg ctgcacccgg cgcggatcga tccacggagc
     1681 gaaacggtgc tcgatgagct cggccagagc tgagcgggga atcaggactc gcggatccac
     1741 acgcatggac aagccggcaa accaggtctc gccggtgaga tatgccgccg gaatccgctt
     1801 cttgatgcgc actgtgatca gcgccgcagc gcgccgccga cccgcttcac tcactcgtcg
     1861 gctgagcacc gagcggctcc cgtgaggcga gtgacccaac gcgtgcagga ccagagtggc
     1921 cgcctcgtcc cgggcgttgt ccgtgccatg cccgaagaac acgcccgcgc gctgcagccg
     1981 gcgggcgctg ctcgcaatga ggtctcgaac caaaaccact cgacctctat gggataattc
     2041 gcgcatgacg caacgcagct cctggattct gctgatcctc gccggcctgc tcgcggcgct
     2101 ggccggcttc ggactgtcac ggaaacttga cagctcgggg ccgcagctcg caagtggcac
     2161 ctggctgccg gcgccgcgcg ctgtcggcga cttccacctt accgatcatc tgggtcagcc
     2221 cttcacggcg cagaatctgc aggggaaagt cagcctggtg ttcttcggtt tcacgcattg
     2281 tccggatgtc tgcccgacca cccttgccaa gctcgcacag gtgcgcaagg tcgccgcagt
     2341 tcccgctctg caggtgctgt tcgtcaccgt ggaccccgag cgcgatacgc ccactgccat
     2401 ggggctctac gtgcatgcct tcgatcctga gttcatcggc ctgaccggcg atacccagga
     2461 gatccagcgg ctgagcgcga aattcggtat tgccgccacg cgcgtggagt tgccaggcgg
     2521 tgactacacg atggatcact ccgcagcggt cttcctgctc gatcggcagg gccgcatcgc
     2581 cgccatcttt accccgccgt tcgacatcca gcgctttgcg ctggacctcc atcagagcct
     2641 gccctacctc gggtcccgct cctgatgact gcgccatgag cgaggaggct ccggcgggaa
     2701 ttcgtgagcg gctcttcgtc gccctgcagc atgtcattcc gcagcacttc ctgtcgcatc
     2761 tggtctatcg gctcgcacgc agccgctcac cgggcctcaa gaacaggctc atccaggcgt
     2821 tcgtgcgaag ttaccgtccc gagatgcgcg aggctgccga accggacccc ctgcgttacg
     2881 cctcgttcaa tgacttcttc acgcgcagcc tgcgcgcggg cagccgacca gtggatcccg
     2941 ctgcgcgctc ggtcgtctcg ccggtcgacg ggttgatcag ccagatcgga catctcgagg
     3001 agtcccgcat cgtgcaggcc aagggacatg actattccgt ggcggagctg ttgggcctgg
     3061 aggccggctc gtcctggccc caacgtttcg ccggcggcag cttcaccacg ctttatctgg
     3121 cacccttcga ctatcaccgc gtccacatgc cactcgaggg gacactgcgc gccgcctggt
     3181 acgtgcccgg acgcttgttc agcgtcaatg cggttaccgc cgcgtcggtg ccgcgcttgt
     3241 tcgcccgcaa tgagcggatc atctgtctgt ttgaaggccg cactgcgccg ttcgcggtgg
     3301 tgctcgtggg agctctgttt gtcggtagca tgagcacggt atggcacggt gagatcactc
     3361 cgcgtagtcc gcctcagcgg ttggacttgc cgctgcaatc cacccacgcg ccactgcacc
     3421 tctccaaagg ggccgagatg ggccggttca acatgggctc gacggtcata ttgctgctcc
     3481 cgccgggcgc gagcacctgg ctgcctgagt ggcgcagcgg ggagcgggtt ctggtcgggc
     3541 aggtgcttgc atacctgggc gcatgaagac cgactcggcc ctttcctggc agcccaccac
     3601 tgcacctctg acattgaagc gccgtgccgc gctgctcgcg cagacgcgaa agttctttgc
     3661 cgaccgtggg gtcctcgaag tggacacccc gatggtcgtg cacgcggcag tgaccgatgt
     3721 ccatatccat tccgcgcggg ttgaactcga gcagggcggc gcggcggatc gccccgagcg
     3781 atcattcttc ctgcacacat cgcccgagta tgcgatgaag cggctcctgg cggcaggcag
     3841 cggcgacatc tatcagatct gccacgtggt gcgcgccttg gagcgcagcg
//
LOCUS       DASRHN010000302         6579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_268215, whole genome shotgun sequence.
ACCESSION   DASRHN010000302 DASRHN010000000
VERSION     DASRHN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268215"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..3441)
                     /locus_tag="VFB37_12110"
     CDS             complement(268..3441)
                     /locus_tag="VFB37_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZO23236.1"
                     /translation="MNGVIAFALKQRTFLAILLPLVLIGGVLGFRQLNIEAYPDPVPP
                     LVDVVTQSAGQSAEEIERYITIPIEVEMTGIPHVSTVRTISLFGLSDVKIQFTYDFSY
                     QEAEQWVINRLSQLPQLPNAAIPQISPTSSVGEIYRYRLVAPRGYSVTDLKTLEDWVL
                     ERRFKAVPGVIDVTGWGGKTKTYEITVDQRRLVQYGLSLSQVLQALNNSNINVGGQTV
                     NIGPQSAVVRGVGLIHSMDDIRKTMVASTRDVPVLIGDIASVHVEHAPRLGIAGYDDD
                     DDVVEGIVLMRRGERSLPTIERVKAEADLINSSGVLPPGVHIERIYDRSDLIRLTTHT
                     VLRNVLAGVLLIFLLQWAFLGNLRSALIVACTIPFALALAIALMLLRGESANLLSVGA
                     IDFGLIVDATVIMVENIFRHLGESADARRAGSPILHHVRGSSIFRGKFAVIANAATEV
                     NQAILFAAAIIIAGFVPLFTMSGIEGHIFGPMARTYGYAIAGGLLATFTISPALSALL
                     LSDRVAEIETPIVRGLRRCYEPMVEFALANRVLTLGSFAILLLVAAFAVRQLGLEFLP
                     KLEEGNFWIRATLPESISLQEGNGYVNQMRRLIRSFPEVSAVVSQHGRPDDGTDATGF
                     FNAEFFVPLRAPGTWPAGVDKDSLTLQLKTALESHFPGVEFNFSQYIQDNVEEAASGV
                     KGENSVKLFGNDLQTLQRTAEKIKGVLGTVPGVTDLAVFDPLGQPTVRIDIDRSRAAR
                     LGLSPGDVNATLQAAIGGQAAGDLYEGGSDRHFPMIVRLAEQYRGDPDAIRRILIGAP
                     DPAGGGSMVQIPLSDVASVRLISGPSFIYREQQERYVPIKFSVRGRDLGSAVLEGQRA
                     VARQVHLPAGYHLEWVGEFGNLQEALQRLAIAVPASLALICALLFINFGSLVDVLLAA
                     SVIPMALIGGVFALAITGTPFSVSAAIGMVALFGIATMDGIIVLSYYNLHIEEGLDRV
                     SALVHTCRVQMRPVMMTCVVACVGLVPAALSTSIGSQVQRPLALVVVGGMLLTPVLVL
                     LVLPVLILLLSRRTPDAERVGHAEPAT"
     gene            complement(3473..4693)
                     /locus_tag="VFB37_12115"
     CDS             complement(3473..4693)
                     /locus_tag="VFB37_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO23237.1"
                     /translation="MDQPTHPPERHALERRALSVRRQTQIVLVLGLCALGILSAGWAA
                     SLLRSQHPAPADELTANGELHLSALQLAGLTINPVSQVSFRSEEETDGRITLNSDTAT
                     QVFSPYSGRVVRVIAGIGEHVGRGAPLFSIEASEFAQTQSDLLNALSQLKLARIGEER
                     RHAAFESKGGSLQDWQQAQADLAAAGTALDSVRNRLRIFGESDAQIAALEREKAPNPV
                     TNVVAPIGGVVTDRQVGPGQYLQAGGATPVYTIGDLSTVWLVAEVREVDAPRVTVGQD
                     VEVRVLALPGRVFRAKLTAVGAGVDPATRRVPVRATIENPDGKLKPQMFATFNIITSG
                     NSESLAVPEEAVVREGDSARVWVLERGNDLALRQIRTGRISNGMVEVLAGLKAGEKIV
                     TRGSLFIDRAAQPG"
     gene            4842..5264
                     /locus_tag="VFB37_12120"
     CDS             4842..5264
                     /locus_tag="VFB37_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZO23238.1"
                     /translation="MSNWVFWALLSACFAALTAIFAKVGVAEINADVATFIRTIVILT
                     VLGVLVLSTGEYRLVSGASSRTWLFLVLSGLATGASWLCYFRALQAGEASRVAPIDKL
                     SVVLVALFGATFLGERLAAVNWLGIALIAFGAWLVTLG"
     gene            complement(5251..>6579)
                     /locus_tag="VFB37_12125"
     CDS             complement(5251..>6579)
                     /locus_tag="VFB37_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO23239.1"
                     /translation="EGLILAVPEVASYSRRTGLQLGGGITEANEGDFFIKLKSGRRRD
                     IEDVMDELRTRAEREIPGLTIETAQLMEDLIGDLTAVPQPVEVKLFGPDLAQLQSLAP
                     QVAKKLSTVDGLTEIQDGLRIAGDAVELHVDRTRAAVEGLDPDGLARQTAALVGGTLA
                     GQIQSGEKLLDIRVWSPATLRDRLDALGELRLRAPDGHEFPLSRVADVRIAAGQPQLQ
                     RENLQQMVAVTARLEGRDLGSAMQQVKVAVSSMNLPPSLRVEYGGLYAEQQRSFRGLA
                     AVFAAAVLLVMTLLLYLYERWAVVLAILATVCLSIAAVFVGLWVTGTELDISALMGLT
                     MIVGMVAEIAVFYFAELTPAGMQAGTMSEEALVTAGRRRLRPILMTSLIAILALLPLA
                     LGLGAGSQMQTPLAIAIICGLLAALPLVLLVMPVFYRRFSRGRGPGLSPG"
BASE COUNT         1166 a         2182 c         2132 g         1099 t
ORIGIN      
        1 aggcgttggc tctcctggtc cgcagtgatc tgcctgggag gggtgcccgg tacatagccc
       61 tggaccttag gcggtgcggg tgatctgaag tcaggcccga cggcacaact tgccagcaga
      121 gcagccgcgg cgagaccggg agcagctgcc gctatccgca gagcccctct cgctatccgc
      181 agcgccgatg acagcgcgta gggagccggt accgctgcgc acgactgtgc gttcgcctct
      241 ggaatcatgt gcccgggtgt cctggaatca tgtggcaggc tccgcgtgtc ccacccgctc
      301 ggcatccggc gtgcggcgtg acagcaacag aatcagaacc gggagcacga gcaggaccaa
      361 caccggtgtg agcaacattc caccgaccac gaccagtgcc agcggccgct gcacctgcga
      421 gccgatgctc gtcgagagtg ccgccggcac gagccccacg catgcgacca cgcaggtcat
      481 catcacgggc cgcatctgca cacggcaggt gtgcacgagc gcgctcacgc ggtcgagccc
      541 ttcctcgatg tggaggttgt agtacgagag cacgatgatg ccgtccatgg tcgcgatccc
      601 gaacagggcc accatgccga ttgcggctga cacgctgaat ggagtcccgg tgattgccag
      661 tgcaaacacg ccaccaatga gtgccatggg gatcacgctc gcggcgagga gaacatcgac
      721 cagcgagccg aagttgatga acagcagcgc gcagatcagg gcgaggctgg ccggcacggc
      781 aatcgcgagc cgctgcaggg cttcctgcag gttgccgaac tcgccgaccc attcgagatg
      841 atacccggcg ggcagatgca cttggcgtgc caccgcgcgc tgtccttcga ggacggcgct
      901 gccgagatcg cggccccgaa cactgaactt gatcggaacg tagcgctcct gctgctcgcg
      961 gtagatgaac gagggtccgg agatcagccg cacgctcgcg acatcgctga gcggaatctg
     1021 caccatgctg cctccgcctg ccgggtcggg cgcgccgatc aggatgcgcc ggatcgcatc
     1081 cggatcgccg cggtattgct cggcgaggcg cacgatcatc ggaaaatgac gatcgctgcc
     1141 cccctcgtac agatcgccag ccgcctgtcc gccgatggcc gcctgcagcg tggcgttcac
     1201 atcacccggg gacaaaccga gccgcgcggc cctgctgcga tcgatatcga tccgcaccgt
     1261 gggctgcccg agggggtcga aaaccgcgag gtcggtgacg ccgggcaccg tgccgagcac
     1321 gcccttgatc ttctccgctg tccgctgcag cgtctgcagg tcgttgccga agagcttcac
     1381 cgagttctcg cccttgacgc ccgaggcggc ctcctcgacg ttgtcctgga tgtactgcga
     1441 gaagttgaat tcgacgcccg ggaaatgtga ttcgagcgct gttttgagct gcagcgtcag
     1501 gctgtccttg tccactcccg ccggccacgt ccccggggcc cgcaacggca cgaagaactc
     1561 cgcattgaag aacccggtgg cgtcggttcc atcatcgggt cgtccgtgct gggagactac
     1621 tgcgctcact tccggaaagc tgcggatcag gcggcgcatc tggttcacgt agccgttgcc
     1681 ctcctgcagc gagatcgatt cgggcagggt ggcgcggatc cagaagttgc cctcctcgag
     1741 cttggggagg aactccagcc cgagctgccg cacggcgaag gcggcaacca gcagcaaaat
     1801 ggcgaagctt cccagtgtca gcacgcggtt ggcgagcgcg aactcgacca tcggttcata
     1861 gcatcggcgc aagccccgta cgatcggagt ttcaatctcc gcgacccgat cggacagcag
     1921 cagcgcgctc agagccggtg agatggtgaa ggtcgccagc aggccgccgg cgatggcata
     1981 gccgtaggtg cgcgccatcg ggccgaagat gtgcccctcg atgccgctca tggtgaacag
     2041 gggcacgaac ccggcgatga tgatcgcggc agcgaacagg atggcctggt tcacttccgt
     2101 cgccgcattg gcgatgaccg cgaacttgcc gcggaatatc gagctgccgc ggacgtgatg
     2161 caggatcgga gagccggcgc ggcgcgcatc cgcgctctcc cccagatggc ggaagatgtt
     2221 ctccaccatg atgaccgtcg catccacgat gaggccgaag tcgatcgcgc ccaccgagag
     2281 cagattagcc gactccccgc gcagcagcat cagcgcgatg gcgagggcaa gggcgaacgg
     2341 aatcgtacag gcgacgatca gcgcactgcg cagattgcca aggaacgccc actgcaacag
     2401 gaagatcagc agtacgccgg caagcacatt gcgcaggacg gtgtgcgtgg tcagccggat
     2461 gaggtccgag cgatcgtaga tccgctcgat gtgcacgccc gggggcagca cgccgctcga
     2521 attgatgaga tcagcctcgg ccttgacgcg ctcgatggtc ggcaggctgc gctcgccccg
     2581 gcgcatcagc acgattcctt cgaccacatc gtcatcgtcg tcatagcctg ctattccgag
     2641 gcgcggtgcg tgctcgacgt ggacactcgc gatgtctccg atcagcaccg ggacatcacg
     2701 ggtggaggcc accatcgtct tgcggatgtc atccatggaa tggatcaggc ctaccccccg
     2761 caccacagcc gattggggcc ctatgttgac tgtctgtccc ccgacgttga tgttgctgtt
     2821 gttcagcgcc tgcagcacct gggacaagga cagtccgtac tgaacaaggc gtcgctgatc
     2881 gaccgtgatc tcataagtct tggtcttgcc accccacccg gtgacatcga tgacgccggg
     2941 tacggccttg aagcgccgct cgagcaccca atcctcgagc gtcttgagat cggtcaccga
     3001 gtagccgcgg ggtgccacca ggcggtagcg gtaaatctcg cccaccgagc tggtgggcga
     3061 gatctgcggg atggcagcgt tcgggagctg cggcagctgt gagagccggt tgatcaccca
     3121 ctgctcggcc tcctgatagc tgaagtcgta ggtgaactgg atcttcacgt ccgagaggcc
     3181 gaacagcgaa atggtccgca ccgtgctcac gtgcggaata ccagtcatct cgacctctat
     3241 cggaatcgtg atgtagcgct cgatctcctc ggccgactgg ccggcgctct gcgtcaccac
     3301 gtccaccagc ggcgggacgg ggtccggata ggcctcgatg ttgagctggc gaaagcccag
     3361 tacgccgccg atgagtacca gcggcagcag aatagccagg aatgtccgct gcttcagcgc
     3421 aaatgcgatg acgccgttca tcaactgacc ggctgctcca acgcagcgcg tctcatccgg
     3481 gctgggccgc ccgatcgatg aacaggctgc cgcgcgtcac gatcttctcg ccggccttga
     3541 gccccgccag cacttccacc atgccattgc tgatgcggcc ggtacggatc tgccgcagcg
     3601 cgaggtcgtt gccgcgctcc agtacccaca cccgcgcgct gtccccttcg cggaccactg
     3661 cctcttccgg cacggccagg gactcggagt tgccactggt gatgatgttg aatgtggcga
     3721 acatctgcgg cttgagcttg ccgtccggat tttcgatcgt cgcgcgcacg gggacgcgac
     3781 gcgtggcggg gtctaccccg gctccgacgg cggtcaactt cgctctgaag acacgccctg
     3841 gcagagccag caccctgacc tcgacgtcct ggccgacagt gacccgaggg gcatcaactt
     3901 cgcgaacctc ggccacgagc cacaccgtcg agagatcccc gatggtgtac acgggcgtgg
     3961 cgccacccgc ctgcagatac tgacccgggc ctacctgccg atcggtcacc acgccgccga
     4021 tcggcgcaac cacgttggtg accggattcg gggccttctc gcgctcgagc gcggcaatct
     4081 gggcatcgct ctcaccgaag atccgcagcc ggttgcgcac cgagtccagt gccgtgccag
     4141 cggccgccag atcggcctgc gcctgctgcc aatcctgcag cgagcccccc ttgctctcga
     4201 aggctgcgtg gcgccgctcc tcgccgatcc gcgccagctt cagctgcgag agcgcgttga
     4261 gcagatcgct ctgcgtctgc gcgaactcgg acgcttcgat gctgaacagc ggcgcgccgc
     4321 gaccgacgtg ctcgccgatc cccgcgatca cgcgaaccac gcggcccgag tacggcgaga
     4381 agacctgggt cgcggtgtca ctgttgagcg tgatgcgacc gtcggtctcc tcttccgagc
     4441 gaaagctgac ctgcgagacc gggttgatcg tgagtcctgc gagctgcagc gcactgaggt
     4501 gcagctcacc gttggcggtg agctcgtcgg cgggcgccgg gtgctgcgag cgcaaaaggg
     4561 aggccgccca gccggcactc aggattccga gcgcacagag gcccaggacg agcacgatct
     4621 gtgtctgcct gcgcacgctg agcgcgcggc gctcgagagc atgtcgctcg ggaggatgag
     4681 taggctggtc catgtcaggg ggacagtccg ggatatcgga cggcgatcgc gactagtgta
     4741 gtggtgcaag gtttcggagg gctttcaggc gcctttcggc aaccttgcgc agcgacgcgc
     4801 aattcacgaa aatcgagcag ccgtcggaga ctgaagaaac tgtgtcgaac tgggtgttct
     4861 gggccttgtt gtcagcgtgc ttcgccgcgc tgaccgccat cttcgccaag gtcggtgtcg
     4921 ctgagatcaa cgcggacgtg gcgaccttca tccgcacgat cgtcattctg acagtgctgg
     4981 gtgtactcgt tctgagcacc ggtgagtatc gcctcgtgag cggcgcatcg tcgcgtacgt
     5041 ggctctttct cgttctatcg ggactggcta ccggggcttc atggctgtgc tacttccggg
     5101 cgcttcaggc gggcgaagcc tcgcgcgtgg cccctatcga caagttgagc gtggtgctgg
     5161 tggcactctt cggtgccact tttctgggcg agaggctggc ggcagtgaac tggctcggca
     5221 tcgcgctgat cgcattcggc gcctggctgg tcaccctggg ctgaggcccg gaccgcgccc
     5281 gcgactgaac cgccggtaaa agaccggcat gaccagcagc acgagaggga gcgccgcaag
     5341 cagcccgcaa atgatggcga tggccagcgg ggtttgcatc tgcgagccgg cgccgaggcc
     5401 gagcgccagc ggcagcaagg cgaggatcgc gatcagggaa gtcatgagaa tgggccgcag
     5461 gcgccgccgc cccgcagtga ccagcgcttc ctcgctcatg gtaccggcct gcatcccggc
     5521 cggggtcagc tctgcgaaat agaacaccgc gatctcggcc accatcccga cgatcatggt
     5581 cagccccatc agcgccgaaa tgtcgagctc cgtacccgtc acccacagcc cgacgaacac
     5641 cgccgcgatc gacagacata cggtcgcaag gatcgccagc acgacggccc agcgctcgta
     5701 caggtacagg agcaaagtca tgacgagcag cacggcggcg gcaaacaccg cggcaagacc
     5761 gcggaaggag cgctgctgct cggcatacag acccccgtac tccacccgca gactcggcgg
     5821 caggttcatc gagctcacgg ccactttcac ctgctgcatc gcgctgccca gatcccttcc
     5881 ctcgagccgc gcggtgaccg ccaccatctg ctgcagattc tcgcgctgca gttgcggctg
     5941 gccggccgcg atgcgcacgt cggccacgcg actgagggga aactcatggc cgtcgggggc
     6001 gcgcaggcgc agctcaccga gggcatcgag tcgatcgcgc agcgtcgcag gcgaccagac
     6061 gcggatatcg agcagcttct cccccgactg gatctggccc gcgagcgttc caccgaccag
     6121 cgccgcggtt tgcctggcga gaccgtccgg gtccagtcct tctaccgcag cgcgcgtgcg
     6181 atccacgtgc agctcgaccg catcgccggc gatccgcagc ccgtcctgaa tctccgtgag
     6241 gccatcgacc gtcgagagct tcttggccac ctgcggcgcg agcgattgca actgcgcgag
     6301 gtccgggccg aatagcttga cttcgaccgg ctggggaacg gcggtgaggt ccccgatcag
     6361 atcctccatg agctgcgcgg tctcgatcgt gagccccgga atctcgcgct cggcgcgggt
     6421 gcgcagctca tccatcacgt cctcgatgtc gcgacgccgc cccgacttga gcttgatgaa
     6481 gaagtcgccc tcgttggctt ccgtgatgcc gcctccgagc tgcagtccgg tgcgacggga
     6541 gtagcttgcg acttccggga ccgccaggat gagcccttc
//
LOCUS       DASRHN010000303         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2246029, whole genome shotgun sequence.
ACCESSION   DASRHN010000303 DASRHN010000000
VERSION     DASRHN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246029"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFB37_12130"
     CDS             complement(<1..462)
                     /locus_tag="VFB37_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23240.1"
                     /translation="MCAILAAPDVLDLEQPPTPVMPSSRLTYRVGFAVAALLAAFQCD
                     ALGNGVTPYLPLNLEPEIESQIERVLILGDQPVMTRPIPAATVLDALPKACTVDEALC
                     QRVRRYLARYMHGTGIAEASLEGASSSGSGDRSVTPNRYGMREDSHWEASAQ"
     gene            636..1724
                     /gene="gmd"
                     /locus_tag="VFB37_12135"
     CDS             636..1724
                     /gene="gmd"
                     /locus_tag="VFB37_12135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZO23241.1"
                     /translation="MSKRALITGITGQDGAYLAELLLTKGYEVHGLKRRASSFNTDRI
                     DHLYQDPHERGVRMYLHYGDLTDATSLIRIVQQVRPDEIYNLAAQSHVAVSFETPEYT
                     ANADALGTLRLLEAIRILGLGASTRFYQASTSEMFGKVQETPQRETTALYPRSPYAVA
                     KAYSHWITINYREAYGLYACSGILFNHESPVRGETFVTRKITRGLTRIHAGLEECLYL
                     GNLDSLRDWGHARDFVRAQWLMLQQPEPDDYVIATGEQHSVREFVTRAAAELAMSIEW
                     RGKGASEQGVDSQSGRVVVKVDPRYFRPTEVDTLLGDASKARSRLGWRPEISFDSLVK
                     EMVQEDLQIARRDAIIAREGFKTYRYQE"
     gene            1762..>2189
                     /locus_tag="VFB37_12140"
     CDS             1762..>2189
                     /locus_tag="VFB37_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23242.1"
                     /translation="MRLILRRFAAVLVALATVMSLQPAGRALAQSGQSGQSPADLSGI
                     LQNLTPEQQQAILDRLSGGGGSGFGGLLGGGGQDQLGLGMRPGQQQLERKQPLSEEDQ
                     EEQEPLIPVMKADDWVIIEIDYRLGPRTIQPTAQALQALQL"
BASE COUNT          407 a          689 c          726 g          367 t
ORIGIN      
        1 ctgtgccgaa gcctcccagt gactgtcctc gcgcatgccg taacgattcg gcgtcaccga
       61 ccgatccccg gagccgctgc tcgaggcacc ctcgaggctg gcttcggcga ttccggtccc
      121 atgcatatag cgcgcgaggt agcgccggac gcgctggcag agcgcctcat cgaccgtgca
      181 cgcttttgga agcgcatcga gcaccgtcgc ggcagggatc gggcgggtca tgaccggctg
      241 atctccgagt atcagcaccc gctcgatctg gctctcgatc tcaggctcca ggttcaaagg
      301 caggtagggg gtgaccccgt tgcccagggc atcgcactgg aatgcagcaa ggagtgccgc
      361 gaccgcgaaa ccgacgcgat acgtgagccg cgaagatggc atgactggcg tagggggctg
      421 ctcgagatcg agtacgtcag gagcagccag tatcgcacac atgcgatgac tcgcctgtcg
      481 ccgctgcgca cccgccgtat gagctgtcaa gcgccgccga ggcgcgatcg gggaagccgc
      541 ggccctcagg cgaagcagcc agcgggtgat gtaacatgcc tgacccgcag cacaggcgcg
      601 accgccccgg tgcagccggg cacagcaggc agggcatgag caagcgcgca ctgatcacag
      661 ggattaccgg ccaggatggc gcctacctcg cggagcttct tctgacgaag ggctacgagg
      721 tgcatggact gaagcgtcgc gcctcctcgt tcaataccga tcgcatcgat cacctctatc
      781 aggatccgca tgagcgcggc gtgcggatgt acctgcatta cggcgacctc accgatgcga
      841 ccagtctcat ccggatcgtg cagcaggtgc gcccggatga gatctacaat ctcgccgcgc
      901 aaagtcacgt cgccgtgtcc ttcgagactc ccgaatacac tgccaatgcg gacgcgctcg
      961 gcacgctgcg cctgctcgag gccatccgca tcctgggact cggagccagc acgcggttct
     1021 atcaggcctc cacctcggag atgtttggca aggtccagga gactccgcag cgcgagacga
     1081 ccgcgctgta tccgcgctcg ccgtacgcag tcgcgaaggc gtactcccac tggatcacga
     1141 tcaactatcg tgaagcctac ggcctttatg cctgcagcgg aatcctgttc aaccacgagt
     1201 cgcccgttcg cggggagact ttcgtgacac gcaagatcac gcgtggactc acccgcatcc
     1261 acgcaggcct ggaggaatgt ctgtatctgg gcaatctcga ttcactgcgc gattggggcc
     1321 atgcccgcga tttcgtgcgc gcacaatggc tcatgctgca gcagccggag cccgatgact
     1381 acgtgatagc gacgggtgag cagcattcgg tgcgtgagtt cgtcacgcgc gccgcggcgg
     1441 agctcgccat gagcatcgag tggcgtggta agggagcgag cgagcagggc gtggacagtc
     1501 agagcggccg tgtcgtggtg aaggtcgatc cacgctattt ccggccgacg gaagtggata
     1561 cgttgctggg cgatgcgagc aaggcgcgca gccgcctggg ctggcggccg gagatcagtt
     1621 tcgattcact ggtgaaggag atggtccagg aagacctgca aatcgcgcgc cgcgatgcga
     1681 tcattgcgcg cgagggattc aagacctaca ggtatcagga gtgagtcggg gcgcgggccg
     1741 ccgatgagga aaccattttc gatgcgtctc attctgcgcc gcttcgcggc tgtgctggtg
     1801 gccttggcca cggtcatgtc tttacagccc gcgggccggg cgctggccca gagcgggcaa
     1861 tccgggcagt ctccggcgga cctgtccggc attctgcaga atctgacacc cgagcagcag
     1921 caggcgatcc tggaccggct ctccggtggc ggcggctcgg gattcggcgg cttgctgggc
     1981 ggcggcggcc aggatcagct cgggctgggc atgcgtccgg gtcagcagca gctcgagcgc
     2041 aagcagcccc tcagcgagga ggatcaggag gaacaagagc cgctcatccc ggtcatgaaa
     2101 gccgatgact gggtcatcat cgagatcgac taccgtctgg gaccgcgaac catccagccc
     2161 accgcgcagg ctcttcaggc gctgcagct
//
LOCUS       DASRHN010000304         4768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_435946, whole genome shotgun sequence.
ACCESSION   DASRHN010000304 DASRHN010000000
VERSION     DASRHN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435946"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFB37_12145"
     CDS             <1..808
                     /locus_tag="VFB37_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO23243.1"
                     /translation="RFAELGFKVSPDTLALMKRMVGSGEVDALVPERVWQETERALGE
                     SRPEVFFETLRQCGALAPIFPELAALHGVPQPARWHPEIDTGVHVMLALRHAARIGAP
                     SSVRFAVLMHDLGKARTPPERWPSHHGHEEAGVPLVQALAQRLKVPSAYRDLAVLAAR
                     HHAVIHRAAELRPDTLLKLLESTDAFRRPERFAELLQACESDARGRTGLEDRPYPQAE
                     YLRKALQAAAAVGLSDEDRIGLQGPALGEKLRAKRLAALSDVKATAGPVS"
     gene            complement(817..1614)
                     /locus_tag="VFB37_12150"
     CDS             complement(817..1614)
                     /locus_tag="VFB37_12150"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZO23244.1"
                     /translation="MSDTLNTFILGIIEGITEFLPISSTGHLLVAEQWLGQRSELFNV
                     AIQSGAILAIVLIYWRRLLALLTRFDDPANRDYFLKLSAAFLITAVGGLIATRLGFEL
                     PKTVVPIAVAFLLGGFAILLIERYIAGKPELVEVTWNVAIWVGACQILAGVFPGTSRS
                     AATIFAGMLAGLTSRPAATEFAFLVGIPTMFAATAYEFMKSRHESAGAEDWNALAIGF
                     VVSGVVAFIAVKWMLRYVQSHRFTLFAWYRIVLGAGLLGLVWKGVLS"
     gene            complement(1731..2924)
                     /locus_tag="VFB37_12155"
     CDS             complement(1731..2924)
                     /locus_tag="VFB37_12155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZO23245.1"
                     /translation="MTASAAHSILPSLSAEERQHSEAAAALIRERLERAGGWLSFEEF
                     MQLALYAPGLGYYSAGSVKLGAGGDFVTAPEISDLFSCCVAWQCAQVLRETGGEILEF
                     GAGTGRMASVILPSLAELGVLPARYAILEVSADLADRQRERIARLPRELRDRVIWLDR
                     LPATPLVGCMLANEVLDALPVHRFVMRAGSLRELGVRPAGAGFAWAERPASAAPDAAF
                     SAAALELLAQLEGRLPEGYVSEIGLQLAPWIAGVGACLHRGMLLILDYGLPCAHYYHP
                     QRADGTLRCHFKQRAHDDPFINIGVQDITAWVDFTRVAQSALAAGLEVGGFATQAAFL
                     LGTGIEAMTAGVTDEVSRVRLAGEARRLLLPGEMGEAVKVMALTRELEIALDGFAHQD
                     LRPSL"
     gene            2935..3693
                     /locus_tag="VFB37_12160"
     CDS             2935..3693
                     /locus_tag="VFB37_12160"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HZO23246.1"
                     /translation="MSTNPSAALAGKTVLITGGARRVGATLVRALHAAGASVALHYRS
                     SGEAATALARELNTVRPESALTLEADLLDLAQLQSLPAATVSAFGALDVLINNASTFY
                     PTPMDDITEIDWNDLIGTNLKAPLFLSQAAAGPLRISSGLILNIADIHGMRPLRRHPV
                     YSIAKAGLIMLTRSLARELGPAIRVNAVAPGPVMWPEDGMDRELQRKIVDNTALKRAG
                     SAEDVARAVLFFATQAPYVTGQVLAVDGGRSISW"
     gene            complement(3702..4214)
                     /gene="folK"
                     /locus_tag="VFB37_12165"
     CDS             complement(3702..4214)
                     /gene="folK"
                     /locus_tag="VFB37_12165"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZO23247.1"
                     /translation="MPNAYVAAGSNVDPVRNLTLASSELRRAFPDVRFSSWYRNTAVG
                     FEGEDFINFVAGFATELPVHALVAQLQEIERLCGRPPGAPRWAPRTMDLDMLLYGEMV
                     CDEPRLKLPRPDLLKRAYMLGPLAELAPEVTHPTTGRSIGELWQQFDRAAHPLHKVEG
                     TTGEQGLHRP"
     gene            complement(4225..4617)
                     /gene="folB"
                     /locus_tag="VFB37_12170"
     CDS             complement(4225..4617)
                     /gene="folB"
                     /locus_tag="VFB37_12170"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZO23248.1"
                     /translation="MPYSGDVIFLRGLAVECVIGFIDWERRVKQTVLLDLELPADCRQ
                     AARYDEVAQTLDYKKVAKRVIAFVEASEFKLVETLAHRLALLLLAEFNLEWLRVSVNK
                     PGAIRGSRDVGVRIERTRSDLQTSAEPG"
BASE COUNT          867 a         1620 c         1509 g          772 t
ORIGIN      
        1 gcgcttcgcg gagctgggct tcaaggtctc tccggacacc ctggcgctca tgaagaggat
       61 ggtcggctcc ggggaagtcg acgcactcgt gcccgagcgg gtctggcagg aaaccgagcg
      121 ggcactcggc gaatcaaggc cggaggtgtt cttcgagacc ctgcgccaat gcggcgcgct
      181 cgcaccgatt tttccggagt tggcggcact gcacggggtg ccgcaacccg cgcgctggca
      241 tcccgagatc gataccggcg tgcatgtcat gctcgcgctg cgtcacgcgg cccggatcgg
      301 tgcgccgagc tcggttcgct tcgccgtgct gatgcacgat ctgggcaaag cgcgaacccc
      361 gcccgagcgc tggcccagcc accatggaca cgaagaagcc ggcgtgccgc tcgtgcaagc
      421 gcttgcgcag cggctcaagg tcccgagtgc ctaccgcgat ctggccgtgc tggccgctcg
      481 acatcacgcc gtgattcatc gcgctgccga gctgagaccc gacaccctcc tcaagctact
      541 ggagtccacc gatgcgttcc gtcgtccgga gcgctttgcg gaactgcttc aggcttgtga
      601 gtcggatgcc cgcggccgca cggggctgga ggatcgccca tatccccagg cagagtatct
      661 tcgaaaggcg ctacaggccg ccgcagccgt cggcttgtca gatgaagatc ggatcggcct
      721 gcaaggtcct gccctcggcg agaaacttcg agccaaacga ctcgcggccc tcagcgatgt
      781 gaaagccact gccggtcccg tgagctgaga tccacgtcag ctcaacacgc ccttccacac
      841 cagcccaagc agtcccgctc cgagcacgat gcggtaccag gcgaagagcg tgaaccggtg
      901 cgactgcacg taacgcagca tccatttcac cgctatgaag gcgacgacgc ccgagaccac
      961 aaacccgatt gcgagtgcat tccagtcttc ggcgccagcg ctctcgtggc gcgacttcat
     1021 gaactcgtac gcggtcgcgg cgaacatggt gggtattccg accaggaatg caaactccgt
     1081 ggccgcgggc cgcgaggtca gaccggccag catgccggcg aagatggtcg cggccgaacg
     1141 ggacgtccct ggaaacacac ccgccaggat ctggcaggcg cccacccaga tggccacgtt
     1201 ccaggtgact tcgacgagct cgggcttgcc cgctatgtag cgctcgatga gcagtatggc
     1261 gaatccgccc aggaggaagg cgacggcgat cgggaccacc gtcttcggaa gctcgaagcc
     1321 gagccgcgtc gcgatcaggc cgcccactgc agtgatcagg aatgccgcgc tgagcttcag
     1381 gaagtagtcg cggttggccg ggtcatcgaa acgggtcaga agcgccagca gccgccgcca
     1441 gtagatcaac acgatcgcaa ggatggcgcc cgactggatc gcgacattga acagctccga
     1501 gcgttgccca agccactgct ctgcgaccag cagatgcccg gtgctggaga tgggcaggaa
     1561 ctcggtgatg ccctcgatga ttcccaggat gaacgtattc agtgtgtcgg acacgatgct
     1621 ccccgtggtc tgcgagccgt aggatggtac cgcctcacct cgagaggtgc cgggagtagc
     1681 tcacaatgac tggcggtggc gcgcctcacc gtgcatcggg caaccgtgcc tcacagtgag
     1741 ggccgcaaat cctggtgcgc gaatccgtcc agggcgatct cgagctcgcg ggtcagtgcc
     1801 atgaccttca ctgcctcgcc catctcgccc ggcagcagca gccggcgcgc ctcgcccgcc
     1861 agccgcacac ggctcacctc atctgtaaca ccggccgtca tcgcctcgat gccggtcccc
     1921 aggagaaagg cggcctgagt cgcgaagcct ccgacctcga ggcccgcagc cagtgccgat
     1981 tgcgccacgc gcgtgaaatc cacccaggcg gtgatgtcct gcacgccgat gttgatgaaa
     2041 ggatcatcgt gcgcccgctg tttgaagtgg cagcgcagcg tgccgtccgc tctctgcggg
     2101 tgatagtagt gcgcacacgg aaggccgtag tccaggatca gcagcatgcc ccggtgcagg
     2161 catgcaccga ctcccgcgat ccatggcgcc agctgcaggc cgatctctga gacatagcct
     2221 tcgggcagcc tcccctcgag ctgcgccaac aattcgaggg ccgccgcgct gaatgcagcg
     2281 tcgggggccg cagacgctgg gcgctcggcc caggcaaaac ctgcgcccgc cggccgaacg
     2341 cccagctcgc gaagggatcc ggcgcgcatc acgaagcggt gaaccggcaa ggcatcgagt
     2401 acttcgtttg ccagcatgca accgaccagc ggcgtggccg gcaggcgatc gagccagatg
     2461 acccggtcgc gaagctcgcg cggcaatcgc gcaatgcgtt cacgctgccg gtcggcaaga
     2521 tcggcactga cttccagaat cgcatagcgc gccggcagca ctccgagctc ggcgagcgac
     2581 ggcagaatga ctgaggccat tcggcccgtg cctgccccga actccaggat ctcaccgccg
     2641 gtctctctca agacctgggc gcactgccat gccacgcagc agctgaacag atcggaaatt
     2701 tccggcgccg tgacgaagtc cccgccggcc ccgagcttca cactgcccgc gctgtagtaa
     2761 ccgagccccg gggcgtacag cgccagctgc atgaattcct cgaacgacag ccagccaccg
     2821 gcgcgctcca ggcgttcccg gatcagcgcc gctgccgctt cggagtgctg ccgctcctcg
     2881 gcagacagcg atggcaggat tgagtgcgcg gcggatgcgg tcataatcgc ggcaatgtca
     2941 acgaatccgt cggctgcact cgccggaaag acggtgctca tcactggggg cgcccgccga
     3001 gtgggcgcca cgcttgttcg cgccctgcat gcggcgggag cgagcgtcgc tttgcattac
     3061 cgcagctccg gcgaagctgc caccgcgctg gcgcgcgagc tgaataccgt acggccggag
     3121 tcggccctca ccctcgaggc tgatctgctc gatctcgctc agctgcagag cttgccggcg
     3181 gcgaccgtga gcgccttcgg cgcactcgat gtgctcatca acaatgcctc caccttctat
     3241 ccgactccga tggatgacat caccgagatc gactggaacg atctgatcgg caccaatctg
     3301 aaggccccgc tgttcctttc gcaagccgcg gcaggtccgc tgcgcatcag cagcggcctg
     3361 atcctcaaca ttgccgatat ccatggcatg cgcccgctgc gtcgccatcc ggtctacagt
     3421 atcgccaagg caggtctcat catgctcacc cgctccctcg ctcgcgagct cggaccggcg
     3481 atccgcgtca atgcggtggc gcctggcccg gtcatgtggc cggaagacgg gatggaccgg
     3541 gagctgcaga gaaagatcgt ggacaatacc gcgctgaagc gcgccggctc cgctgaggat
     3601 gtcgcgcgag ccgtgctgtt cttcgcgacc caagccccgt atgtcaccgg tcaggtactg
     3661 gcggtggacg gcgggcgcag catcagctgg tagtcgtccg ctcaaggacg gtgcagcccc
     3721 tgctcgccag tcgtcccttc taccttgtgc agcggatgag cggcgcgatc aaactgttgc
     3781 cagagctcac cgatactccg acccgtcgtg ggatgagtga cctcgggtgc gagctccgcg
     3841 agtgggccca gcatgtaagc gcgtttgagc agatcgggcc ggggaagctt cagccgcggc
     3901 tcatcacaca ccatctcccc gtacagcagc atgtccaagt ccatcgtgcg cggcgcccat
     3961 cgaggtgcgc ccggcggcct gccgcacagc cgctcgatct cctgcagttg cgccacgagc
     4021 gcatgcaccg gcagctcggt cgcgaagccg gcaacgaagt tgatgaaatc ctctccctcg
     4081 aagccgaccg ctgtgttgcg ataccaggac gaaaagcgta cgtcggggaa agcccgccgc
     4141 agctcgctgc tcgccagagt caggttccgc accgggtcca cgttgctacc ggctgcgacg
     4201 taagcgttcg gcattcgccg tgcctcagcc cggttccgcg gaggtctgca ggtctgacct
     4261 ggtgcgctcg atgcgcactc ccacgtcccg tgagccgcgg atcgcgcccg gcttgttcac
     4321 cgacacgcgc agccattcca gattgaactc ggcgagcagc agcaaggcga gccggtgggc
     4381 gagcgtctcg acgagcttga attctgacgc ctcgacgaac gcgatgactc gcttggcgac
     4441 cttcttgtag tcgagcgtct gcgccacttc atcgtagcgg gcagcctggc ggcagtctgc
     4501 cggcagctcc agatcgagca gaacggtctg cttgacgcgc cgctcccagt cgatgaaacc
     4561 gatgacgcac tccacggcga gcccgcgcag gaaaatgaca tctccggagt aaggcatggg
     4621 gcgctcagat cgtttgcaga gattgcagcg gccactgcgc gcgcgcctgg atacccaggg
     4681 tgtcacgctg gccggcaccg agccgtgcca ggcctgccac agcgatcatg gcagcattgt
     4741 ccgtacaaaa ctcgatccgc ggatagta
//
LOCUS       DASRHN010000305         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1092367, whole genome shotgun sequence.
ACCESSION   DASRHN010000305 DASRHN010000000
VERSION     DASRHN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092367"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFB37_12175"
     CDS             <1..573
                     /locus_tag="VFB37_12175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO23249.1"
                     /translation="FDRDYYKLYYYDPDTAVTGRREMRARARLIAAFAEHAGQSVRRI
                     LDAGCGTGLLRSPLKRLLPQAAYTGIETSDYLCRRYGWVQARIESYRSQMPYDLVVCY
                     DVLQYLDERTAARALANLGRLCRGVLYFTALTRLDWDLNCDRSRTDPNVYLRSARWYR
                     TRLNKSFREAGAGFWLRRGAPLTLWDLESV"
     gene            complement(613..2040)
                     /locus_tag="VFB37_12180"
     CDS             complement(613..2040)
                     /locus_tag="VFB37_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO23250.1"
                     /translation="MSSFDMEPRRGLTLLHAASLVVGITIGTGIFLKSAVMAQAVGSP
                     ALVLGAWIVAGVIALFGALSFAELGALLPRAGGEYVYLRAAFGEIPGFLYVFNSFLVG
                     GASISAYGAAVAIFLGDIYHLGDVWFSRTVHLFGATYTLELGMRQLVAVAVIVFFAAI
                     NCAGVLLGGRVQTVLTAAKVVSVVAVAGGVFLFSASADWHHLASSAGSGGGGLSGFGA
                     AMFAALWAYSGWQFLPMAASEVQKPERNLPRAIIGGTLLVLAIYLLINVAYLYALPFE
                     EVSTSNSTAYPQAPSVAARAVQTFLGVRAAPIAAVIFLVSSIGALNGTILGRARVPFA
                     AARDGLFFEQFGRLNPATRVPVISIVWVSVWAVLLAASGTFDQLTNMAVLSYAIFWIP
                     VVLAVIVLRRKLPDAPRTYRVWGYPFVPLAFVLVMAWIGVNELVTRPLESAATLILIL
                     LGLPLYPVFRRRRSLSLNPPAASSN"
     gene            2104..2865
                     /locus_tag="VFB37_12185"
     CDS             2104..2865
                     /locus_tag="VFB37_12185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO23251.1"
                     /translation="MTLRMTFKDHFSRLASEYSAFRPDYPAALFGYLAGLCREHRAAW
                     DCACGSGQASAGLAAHFEQVIATDASAQQVAAAAAHPRITYRVAAAEHSGIESDSVDL
                     VTVAQALHWFDLDAFYAEVRRVLRSSGILACWTYGVLRVEGHAIDDLVREFYTDIVGP
                     YWPPERRLVDEGYRELSFPFPELDAPALQMQERWDRPHLLGYLRTWSGTARYVEQVGR
                     DPVEDLGKRLASFWPDSHSDRLIRWPLALRVGRKA"
BASE COUNT          556 a          992 c         1015 g          491 t
ORIGIN      
        1 ttcgatcgag actactacaa actctactac tatgaccccg acaccgccgt gaccgggcgg
       61 cgggagatgc gcgcgcgcgc tcgcctgatt gccgcattcg cagagcacgc cgggcaatcg
      121 gtcaggcgaa tactcgatgc aggctgcgga acgggactgc tgcgctcacc gcttaagcgc
      181 ttgctcccgc aggctgcgta caccggcatc gagaccagtg actatctgtg ccggcgctac
      241 ggttgggttc aggcccgtat cgagagctac cgttcgcaga tgccctacga tctcgtggtg
      301 tgctacgacg tgctgcaata cctcgacgag cgcacggcgg cacgcgcgct ggcgaatctg
      361 ggacgtctct gccgcggcgt cctgtatttc accgccctga cccgcctgga ttgggatctc
      421 aattgcgacc gcagccgcac ggacccgaat gtttatctgc gcagcgcccg ctggtaccgg
      481 acccggctca acaagagctt ccgggaagcc ggcgccggct tctggttgcg ccgtggcgcg
      541 cccctcaccc tgtgggacct cgagtcggtg tgatgctgcg ccggggcgcg tccccgagcg
      601 gggcccgcga gctcaattcg atgaggcagc gggcggattg agcgagagtg atcgacgcct
      661 gcggaacacc gggtacaggg gcagacccag caggatcagg atgagcgtcg ccgccgattc
      721 cagagggcgt gtcaccagct cgttcacgcc gatccaggcc atcaccagca cgaacgcgag
      781 cggcacgaac gggtagcccc agactcgata ggtgcggggc gcatcgggaa gcttgcgccg
      841 caggacgatc accgccagca ccacgggaat ccagaagatc gcgtacgaca gcaccgccat
      901 gttggtgagc tgatcgaatg ttccggaggc cgcgagcagc accgcccaga cactgaccca
      961 gacgatggag atcaccggca cacgcgtggc gggattcagg cggccgaact gttcgaagaa
     1021 gaggccatcc cgcgccgcgg caaagggcac acgtgcccgt ccaagtatcg tgccattgag
     1081 tgctccgatg gaggagacga gaaagatgac ggcggcgatg ggcgccgccc ggacgccgag
     1141 gaaggtctga acggccctcg cagctaccga aggggcttgc gggtaggcgg tggagttgga
     1201 ggtgctcacc tcctcgaagg gcagggcgta cagataggca acattgatga gcaggtagat
     1261 ggccagcacg agtaaggtgc cgccgatgat tgcgcgcggc aggttgcgct cgggcttctg
     1321 cacctcgctt gccgccatgg gcaggaactg ccagccgctg taggcccaca gggctgcgaa
     1381 catggctgca ccgaatccgc tcagcccgcc gcccccgctg cctgcgctgc ttgcgaggtg
     1441 gtgccaatcg gccgaagcgc tgaacaggaa tactccgcct gcgaccgcca ccacggatac
     1501 gactttagcg gcggtgagaa cagtctgaac ccggccgccg agcagcactc ccgcgcagtt
     1561 gatggccgca aagaacacga tgaccgccac ggccaccagc tgcctcatgc ccagctcgag
     1621 ggtgtaggtc gctccgaaca aatgcaccgt gcgcgagaac cagacatcgc cgaggtgata
     1681 gatgtcgccc aggaagatgg ccacggccgc accgtatgcg gagatgctgg ccccgccgac
     1741 cagaaagctg ttgaacacat agaggaagcc gggaatctcg ccgaaagcgg cgcgcaggta
     1801 gacatactcg ccgcccgcac gcggcagcag agcgccgagc tcggcgaagc tcagcgcacc
     1861 gaacagagcg atgactcccg ccacgatcca tgcgcccagg accagggcag ggctgcccac
     1921 ggcctgcgcc atgacggccg acttcaggaa gatgcccgtg ccgatcgtga tgcccacgac
     1981 cagtgaggcc gcgtgcagca gagtcaggcc ccggcggggt tccatgtcga acgagctcac
     2041 ggatgctcct ctgcgtccgc gcgcggtgcg caaggctctg cagcctaccg gattgaacgt
     2101 acaatgacct tacgcatgac attcaaggat cacttctcgc ggctggccag cgagtattcg
     2161 gcgttccggc ccgactatcc cgccgctctc tttggttatc tcgctggcct gtgccgcgag
     2221 catcgcgcag cgtgggattg cgcgtgcggc agcggccagg cctcggcggg tctcgcggcg
     2281 cacttcgagc aggtgattgc cacggacgcc agtgcccagc aggttgccgc cgcggcggcc
     2341 cacccgcgca tcacctatcg cgtggcggcc gccgaacata gcggcatcga gtcggattcg
     2401 gtggatctgg tgacggtcgc acaggccctt cactggttcg acctcgatgc gttctacgcc
     2461 gaggtcaggc gcgtgttgcg cagttccggg atccttgcgt gctggaccta tggtgttctt
     2521 cgggtcgaag gccatgcgat cgatgatctc gtgcgggagt tttacacgga catcgtaggt
     2581 ccctactggc cgccggagcg ccggctggtg gacgaaggct atcgagagct gagctttcct
     2641 ttcccggagc tggatgcacc ggccttgcag atgcaggagc gctgggatcg ccctcacctg
     2701 ctcggttatc tgcgcacctg gtcgggaacc gcccgctacg tcgaacaggt cggccgcgat
     2761 cccgtggagg atctgggaaa gcgcctcgca agcttctggc cggactcgca cagcgaccga
     2821 ttgatccgct ggccgctggc tctgcgtgtg ggccgcaagg cttgagccgc gtgctgcaac
     2881 agctcattgc agcgcgatgc aaatgacggc atcacgagcg gtgatcgctt tcagtggcgc
     2941 agtccgctgc cgccgcgaag acggcacgct cggcctcata ctcgagggcc gcaccgcgga
     3001 tgcgcctcat caggaggtca gcctggtgtt ctcgctgggc gccggggtgc cgcc
//
LOCUS       DASRHN010000306         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2409891, whole genome shotgun sequence.
ACCESSION   DASRHN010000306 DASRHN010000000
VERSION     DASRHN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409891"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="VFB37_12190"
     CDS             <1..1936
                     /locus_tag="VFB37_12190"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZO23252.1"
                     /translation="WALRRGAIPAGRVTSNAGALEHKPATSTINCTVSGTIRDSMDDI
                     LKKVHEAGLTLKSGCGIGYEFSTLRPRGAYVTGAGAYTSGPLSFMDIYDKMCFTVSSA
                     GGRRGAQMGTFDVGHPDAMEFIRAKRENGRLRQFNLSLLITDDFMEAVRADREWKLAF
                     PLSQREYEVEQPDLSDGSKYLWREWPVHENYVVNDEGLVACKVYKTLPARRMWDVIMS
                     STYDFAEPGFILIDRVNEMNNNWWCEDIRATNPCGEQPLPPYGSCLLGSVNLTRFVRH
                     PFTEFADFDWAEYRAVVKAFTRMLDNVVEVNGLPLEKQRGEIVRKRRHGMGFLGLGST
                     ITLLGMKYGSPASVTFTEDVSREMAVAGWEAGLELAREKGPAPIMNEEFTVTKEMLRK
                     RPEMARDGWKPGARIAGRLLHARYSRYMQRVAQVAPQLVHELAEVGARFTHHSSIAPT
                     GTISLSLANNASNGIEPSFAHHYFRNVIRQGRKTKEKIDVFSFELLAYRQMVNAKAMP
                     DASASAEKLPDYFTAADEVTPRQHVEIQAAAQRWVDSSISKTANVPTDYKYENFKDIY
                     LYAHEKGLKGCTTFRFNPEAFQGVLVKEEDLKNTTYVFTLEDGTEVEAKGDEQIEYDG
                     ETHTAANLFDAIKEGYYGKL"
     gene            1977..2702
                     /locus_tag="VFB37_12195"
     CDS             1977..2702
                     /locus_tag="VFB37_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23253.1"
                     /translation="MKIDKRIVKYRVRKPEEKPAETKIASAPANDPEVFRDKNGRTAT
                     VIRMHEKLERPEVLIGSTYKIKTPVSDHAMYVTINDIVLNEGTDYEQRRPFEIFINSK
                     NLDHFQWIVALTRIISAVFRKGGDVTFLVEELKAVFDPRGGYWQPGGKFMPSIIAELG
                     YVIEKHLQTIGLMRKPKLDEHQQKLVDEKRAEFEARSKQTDAFSKSHFPEGAQLCAKC
                     STAAVVMMDGCMTCLNCGESKCG"
     gene            3485..>4084
                     /locus_tag="VFB37_12200"
     CDS             3485..>4084
                     /locus_tag="VFB37_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23254.1"
                     /translation="MNSTWGSRAARTPYTRWLLAGALALLAHARIFAAEDSMPRPPQL
                     ERDVQFWIRVYTEVDTNAGFLHDEHNLAVVYDTLHFAPNTSPHEREKRVEEEREHYAA
                     ALRRIAAANGAPLSPEDQHILDMWGEEGTPARLLEATDEIRFQLGQADRFRAGLIRSG
                     AWETHIAETLANLGLPAELAVLPHVESSFNPAARSKVGAA"
BASE COUNT          796 a         1278 c         1292 g          718 t
ORIGIN      
        1 gtgggccttg cgccgcgggg ctattcccgc cggacgggtc acttcaaatg ccggagcgct
       61 cgagcacaag cccgccacgt ccaccatcaa ctgcacggtg tcgggcacga tccgcgattc
      121 gatggacgac attctcaaga aggtccatga ggccgggctg actctcaaga gcggttgcgg
      181 aataggctac gagttctcga cgctgcgtcc gaggggcgct tacgtcaccg gggcgggtgc
      241 ctacacctcc gggcccctgt cgttcatgga tatctacgac aagatgtgct tcacggtgtc
      301 ttcagccggc ggccggcgtg gcgctcagat gggcacattc gacgtcgggc accccgatgc
      361 catggagttc atccgcgcga agcgggagaa cgggcgtctg cggcaattca acctgtcgct
      421 gctgatcacc gatgacttca tggaggcggt acgcgcagat cgtgaatgga agctcgcctt
      481 cccgctgtcg caacgcgaat acgaggtcga gcagccggat ctgagcgacg gctcgaagta
      541 tctctggcgc gaatggccgg tccacgagaa ctacgtggtc aacgatgagg gcctggtggc
      601 atgcaaggtc tacaagaccc tgcctgcccg gcggatgtgg gacgtgatca tgtcctcgac
      661 gtacgacttt gcagagccgg gtttcattct catcgaccgt gtcaacgaga tgaacaataa
      721 ctggtggtgc gaggacattc gcgccaccaa tccctgcggt gagcagccgc ttccgcccta
      781 cggctcctgt ctgctcggct cggtcaatct gacgcgtttc gtgcggcatc cgttcacgga
      841 gttcgccgat ttcgactggg cggaataccg cgcggtcgtc aaagccttca ctcggatgct
      901 cgacaacgta gtcgaggtga acggcctgcc actggaaaag cagcgcggcg agatcgtgcg
      961 caagcgccgc cacggcatgg gtttcctggg cctgggcagc acgatcacgc tgctcggcat
     1021 gaagtacggc tcgccggcat cggtgacgtt caccgaggat gtctcgcgcg agatggcggt
     1081 ggcgggctgg gaagctggcc tcgagctggc acgcgagaag ggaccggcgc cgatcatgaa
     1141 cgaggagttc accgtcacca aggaaatgct gcggaagcgg ccggaaatgg cgcgcgacgg
     1201 ctggaagccc ggagcgcgga tcgccggacg cctgctgcac gcccgataca gtcgttacat
     1261 gcagcgtgtc gcccaagtcg cgccgcagct ggtgcacgag ctcgccgaag tgggcgcccg
     1321 gttcacgcac cacagctcga tcgccccgac gggcaccatt tccctgtcgc tggccaacaa
     1381 tgcctcgaac ggcatcgagc cgtccttcgc gcatcactac ttccgcaacg tcatccgcca
     1441 gggaagaaag acgaaggaga agatcgacgt gttctcgttc gagctgctgg cctatcggca
     1501 gatggtgaac gcgaaggcga tgcccgacgc cagcgcctct gccgagaagc tgccggacta
     1561 cttcaccgcc gccgatgagg tgactccgcg tcagcatgtg gagatccagg ccgcggcgca
     1621 gcgctgggtg gactcttcca tctccaagac agccaacgtg ccgaccgact acaagtacga
     1681 gaacttcaag gacatctatc tctacgccca tgagaagggc ctcaagggct gcacgacctt
     1741 ccgcttcaat cccgaggcct tccagggcgt gctcgtgaag gaagaggacc tcaagaacac
     1801 cacttacgtg ttcacgctcg aggacggcac cgaggtcgag gccaagggcg atgagcagat
     1861 cgagtacgac ggcgagacgc acacggctgc gaaccttttc gatgcgatca aggaaggcta
     1921 ctacggcaag ctgtagctcc cggaaacgga gcgaattccc atgaagagcg agtcccatga
     1981 agattgacaa gcgaatcgtg aaataccggg tccgcaagcc cgaggaaaag cccgcggaga
     2041 cgaagatcgc ttccgcaccg gccaatgatc cggaggtctt ccgtgacaag aacggccgga
     2101 ccgccacagt catccgcatg cacgagaagc tcgagcggcc ggaggtactg atcggatcga
     2161 cctacaagat caagaccccg gtctccgatc atgcgatgta cgtgacgatc aacgacatcg
     2221 tgctcaacga gggcaccgat tacgagcagc gccgtccctt cgagatcttc atcaattcca
     2281 agaatctcga tcactttcaa tggatcgtgg cactgacgcg catcatctcg gccgtgttcc
     2341 gcaagggggg cgatgtcacc tttctggtcg aggagctcaa agcggtcttc gatccgcgcg
     2401 gcggctactg gcagcccggc ggcaagttca tgccctcgat cattgccgag ctcggttacg
     2461 tcatcgagaa gcacctgcag accatcgggc tgatgcgcaa gcccaagctc gatgagcacc
     2521 agcagaagct cgtggacgag aagcgcgccg agttcgaggc ccgctcgaag cagaccgatg
     2581 cgttctcgaa atcgcatttt ccggagggcg cgcagctgtg cgccaaatgt tcgaccgcag
     2641 ccgtggtgat gatggacggc tgcatgacct gcctgaactg cggcgaatcc aagtgtggct
     2701 gaacaggcct gacggcctgc gggagcacaa taccctccac cggatcagac gtcttctcgc
     2761 gtgacagtgc gcgccacagt ccggcagccc gccggctcgc ccgatccagg gcgagggtgg
     2821 cgtttcgaag gcagcccgcg cggctgcctc atttttttcc gacccccgaa tacacagcgt
     2881 gcgcgttgcg ttcgagcgag cccccttgcg cccgagtgct tttcccgggg gcgatccaat
     2941 gcaatgcgcc cgccggccgc ttggcagagc cttgcggctg agccctcttc ccgggggcga
     3001 ccgacacgca acacatccga tagcgcgaaa aaaaccccgc ttgcgcgggg tctatcggtc
     3061 gcttgcttgt gtggaggagt gttttctcgt tgttatccgg tggctgggta tccggttggg
     3121 cgagggcccc aaccggatcc gcgctttctt cgaatcccta gcgtgtttcc ttccgggcgg
     3181 cagactatct gaccgcatct ggatatacat gaagcaacgc gcgtgccagc ccgagcggcg
     3241 cggcattcgg catgacatgt tgtatctgcg acgtagttca agtgtgacgc agtgcgcgtt
     3301 atgcgaccgt ttcaccctcg tcgcggcatg agcgtcggac actccggcgg ccacgcgggc
     3361 ggtcggtagg gcagccgttg tcaagatcat cgacgcattt gcgatcccta tcctcgcgcg
     3421 ctcgggatgc acggcgcaca aaccgttgaa tgggtggacg aatcttggat ttcccggggt
     3481 gtaaatgaac tcgacatggg ggagcagggc ggcgcggacg ccctacacac gatggctgct
     3541 cgccggcgca ctggcattgc tggctcacgc gcggattttt gcagccgagg attcgatgcc
     3601 acgtccgccg cagctcgagc gggatgttca gttctggatc cgcgtttaca cggaggtcga
     3661 taccaacgcc ggcttccttc acgacgagca caatctcgcg gtcgtctacg acaccctgca
     3721 ctttgcgccg aacacctccc cgcacgagcg cgagaagcgg gtggaggagg aacgcgagca
     3781 ctacgctgcg gcattgcggc gcatcgcggc ggcaaatggc gctccgctgt ctcccgagga
     3841 ccagcacatc ctggacatgt ggggcgagga ggggaccccc gcgcggctgc tggaggcgac
     3901 cgacgaaatc cgcttccagc tcggccaggc cgaccggttc cgggccgggc tgatccgctc
     3961 gggcgcatgg gaaacccaca ttgcggaaac gctggccaac ctgggcctgc ccgcagagct
     4021 cgcggtgctg ccgcacgtcg agtcttcctt caatcccgcg gcacgctcca aggtcggggc
     4081 agcc
//
LOCUS       DASRHN010000307        12980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_773702, whole genome shotgun sequence.
ACCESSION   DASRHN010000307 DASRHN010000000
VERSION     DASRHN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773702"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VFB37_12205"
     CDS             complement(<1..1279)
                     /locus_tag="VFB37_12205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZO23255.1"
                     /translation="MLFNSADYAFFLPLVLIIYVLLQRGPLRMQNLWLLIASYVFYGW
                     WDWRFLFLLFGTTLNDYLVGLGLVRVSAPAQRRALLMLSIGVNLGVLGFFKYYNFFIS
                     SLIEAFAGLGVHLHAPTLKVILPVGISFYTFQALTYTVAVYRRQMQPTRDPIAFFAYV
                     AFFPQLVAGPIERAVHLLPQFYRPRTLSPDAFADGVRQILWGLFCKLVVADNCAVVVD
                     TVFDHSGTLPGSVVLVGSLVFAFQIYSDFSGYSHIALGTARLFGFELMQNFATPYFSQ
                     SPVEFWRRWHISLSTWFRDYVYIPLGGSRASRPRQAFNLIVTFVLSGLWHGANWTYIV
                     WGLLNGLYVVPRIFTGDPLAGAVRSRNPLVRLPTVALRIGGTFALILLAWIFFRARDI
                     STAGHLLRELFSRSLFSSPAATLRSMGVLGSALY"
     gene            1411..3123
                     /locus_tag="VFB37_12210"
     CDS             1411..3123
                     /locus_tag="VFB37_12210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZO23256.1"
                     /translation="MSIHRNLRKLGSEPFDVLVIGGGASGAATAREAALRGFNTALIE
                     RGDFSTGASAHCFKVVHGGIRYIQHGDVPRLRASCLERTILLRIAPHLVAPLPFVIPT
                     YGHGKSSRWLLGTGMLMYDSLTADLRRQVEDPARRIQRTRFLSRSETLELFPDLASQG
                     LTGAAVFEDGQMYNPPRLVLAFAAAAAELGATVANHVEAERLLVDGGRVVGIQAVDRL
                     EGTELEIRARLVINAAGPWSEGLLQESALQYGAQGTYSRDACFTVARKYSTPMALAVQ
                     GRTRDSDALLARNSRHLFMVPWRDCTLVGVWHVVVPREPDTVGLPPEELQEFIEEFNS
                     SYPALHLSESDVRRVDFGLVPFGEASRQQQGLSFGKQSRLIDHRQFGLAGLISLISVR
                     YTVARRDASEALDLATAQLGPRSLGKGSAYLPLPGGDIKRFDATLADLAQRRPSWFPA
                     AAVDGLARNYGTRAAEVLSLGEREPPLRRCFTGTQVTHAEAVHGVRAETAQRMTDVVF
                     RRTELGTGGHPGTAALDEMQALLARELSWSDRRTVEERTAVELEFARYLACPPQQAAR
                     ARSA"
     gene            3127..4134
                     /locus_tag="VFB37_12215"
     CDS             3127..4134
                     /locus_tag="VFB37_12215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO23257.1"
                     /translation="MTEDALPKILITGGSGFIGARLAQLATQLGYRVTVTTAVNNNTE
                     RARCDALAKAGIAVIVAPLEEASTLERALAGQDVVIHLAAAQHEAQAPESHFHRVNVE
                     GTQRLLEIASRTGVRRFVHGSTIGVYGQASDGMLDERSPLAPDNPYGRTKAAAEQIVR
                     QGAGKLEYCIIRISETYGPGDMRLLKLFRGIRKGRFLIVGPGTNLHQLIYADDLARGL
                     LAACASGRANGETIVLAGSERISTSEMVAAVGAAVGRNKQLPRAPMWPFVAAASVLES
                     TMTPLGLKPPLHRRRLDFFRKSFSFSTEKAQQLLDFRAQVSFAEGARRTAQWYSANGF
                     L"
     gene            4131..5636
                     /locus_tag="VFB37_12220"
     CDS             4131..5636
                     /locus_tag="VFB37_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23258.1"
                     /translation="MSGESTLVLPGVAAKRSAMITALVLTVLCLLALPEITGRPVVKD
                     AGESLRIALHVEHDGVYSENPRPPYGPTMLREPVPIMVTVAEIWAMDAVLGRAEPAAY
                     FSGDRARYVKLQNLLWLGLLMAAAAGSAAFFGLSVPWQIAAAIAVELPFTVLVPPGTR
                     YAIGLDSLATELAGAALLCTGSLVLTIGFARRRVRTLALASFVFGLLALTKAAFFYIY
                     PLVLASTAVLYAAVTPAARWIRKQTALALLALALPFLVLCSAWMVRNHAQTGYFQMAE
                     RGGPIVLYRGLLDGMTHEEYAGSFYAWAASGTLQRVFGILTGFTPPDLEAGGRLQHLA
                     MGLPGAAGQRDLENEDEGRPELALTWYRQSRAVYERYRRAEIAAGARYPPGAADRKTE
                     ADGFKLIAGRRWMHFALFVPLLLRGATVIFPLLVLTLVYARAARRGELAVFILPALGF
                     VLFYAMITHFVQRYGWLPRPASTIALIVIVAELWRHGWPRTAAERASAQPG"
     gene            5712..6686
                     /locus_tag="VFB37_12225"
     CDS             5712..6686
                     /locus_tag="VFB37_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO23259.1"
                     /translation="MNPVIAVLVPCYNEQAAIASVVRDFRAALPGAAIFVYDNNSTDQ
                     TRAVASAAGAIVRSEPLRGKGNVVRRMFADIEADVFVLVDGDDTYDASSAPKLVQLLL
                     AESLDMVNGSRVASSDLAYRRGHQFGNAMLTGLVARIFGNRLTDLLSGYRVLSRRFVK
                     SFPALTPGFEIETEMTVHALELRLPIAEVPTTYRERPSGSASKLRTYHDGIRILRTIL
                     ILMKQEKPLSFFGLVGALLALLSAGLAIPVVHTYLSTGLVPRLPTAVLSMGIMLLAFL
                     SFFCGLILETVTRGRVEAKRLHYLSIPIRFTSVASQEAEAPWRTGSDR"
     gene            6662..7081
                     /locus_tag="VFB37_12230"
     CDS             6662..7081
                     /locus_tag="VFB37_12230"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZO23260.1"
                     /translation="MAHGLRQIIRQFLAFGTVGVAGFVVDAGVLTLMLRLTGAGPYLG
                     RVVSFLCAATFTWALNRVFTFRDQGTHHTLGAQWLRFLGANAIGGFVNFGVYSLLLAT
                     VAFFTAHPVAGVAAGSLAGLAVNFTLSRMFVFSASTR"
     gene            7568..8668
                     /locus_tag="VFB37_12235"
     CDS             7568..8668
                     /locus_tag="VFB37_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO23261.1"
                     /translation="MAGTVAGPELTIVIPTLNEEGNIEALVAKLHAVLRGINWEVMFV
                     DDDSRDRTRSVTTRLARSDGRVRLLHRIGRRGLASACIEGVQASTAPYVAVMDADLQH
                     DEALLPRMLEVLRGGTTDIVVGSRYVAGGDVEGWESGRARMSNLATRLGQVILRTHVA
                     DPMSGFFMLRREAFDLTVRHLSAVGFKILMDILASAERPLRVEELPYHFRPRVAGESK
                     LDAMVAWEYLILLADKTVGRFVPVRFVMFAMIGLLGLGVHLLVLRLGLSLLELRFTAA
                     QATATGIAMVANFTLNNWLTYRDRRLKGWGFVRGLLSFAFVCGIGAAANVSLASFLFG
                     RQESWWLAGIAGAAMSSVWNYAVSAVLTWKAR"
     gene            8653..10161
                     /locus_tag="VFB37_12240"
     CDS             8653..10161
                     /locus_tag="VFB37_12240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO23262.1"
                     /translation="MESALASGGPGTRVATAQPWESDLARLLQAAVLGAMALGIIATM
                     LGPLHRMPLWMPRDENEGWNAYQALAALSGSLLYPPGNAFITNNYPPLSFYIVGALGR
                     VLGDNLVAGRLIALLSELAVAGNIFLLVRILRGERFFALFSSLLFLLYIGANAQSYVA
                     MDDPEWLAHAFVTGGAVLFLRAQSTWRPAVSLLLSGLLCVAGVLVKHNVIVLPFVVFL
                     WALLNDRRQLLAWVAIGSILGAVSLLLMTDAYGVVFLTDVLGHKRGLSLHKLAGDSVR
                     LLGPMTPLLLAAALLGAFAWRETSMRLVLTYALVGGAIGLWFLAGVGIDVNVLFDLII
                     ALSAAAGLFARRLVPMFAPEHRRLAPALITAVMAALCAPTAIEATVSGLDIIRQDRLQ
                     SGHYRELIAAIAAAPGPVACEQPSLCYWAGKPFEIDFSNYGKKMHAGVGGPEVLRQRF
                     DAGYYAYIQTTGHPAEVRNLTRHYLGDALARQLDQRYVAVRQVNEHYLLAPR"
     gene            complement(10178..10951)
                     /locus_tag="VFB37_12245"
     CDS             complement(10178..10951)
                     /locus_tag="VFB37_12245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO23263.1"
                     /translation="MNANLRPEPLKPGSGVPEPQLAARLEPFDSYWQAPKDVEKGFRS
                     FTAYYRANYLAHMPADRSARILVISCGPGYLVQMLRDCGYRSVLGIDSDPAKVEIARA
                     HQLPCETARAFPFLQGKQAQFDVIIPEQELNHLTLDETVDFLRLCRQALRPGGRIVVY
                     AMNGANPLVGSENLSHNIDHFYNVTEYSLEQILKLAGFESVRVFALKLYVFWKNPLNY
                     VGLAVTATLEFMFRVIFMLYGKKVRVLSKKVAAVASAPG"
     gene            11040..12221
                     /locus_tag="VFB37_12250"
     CDS             11040..12221
                     /locus_tag="VFB37_12250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO23264.1"
                     /translation="MRILFCNYEYPPLGGGGGVVMAAMARTLARRHEVTALSSRAAGL
                     PAEELDGGVRVVRVPVLFRRKLAVANFPSMAAYLPLGLWRGLQLGREQRFDVINTHFA
                     VPTGPLGDWLSRRLGVPNVLSVHGGDLYDPSKGSSPHRHAFLRRAVARLLRRADLVVA
                     QSRDTARNVAQIYGVDREVELIPLGIDRPPQGIQAGRARFDLPEDAFVLVTAGRIVAR
                     KASTQLMDTLAAVPDAYLLVVGDGPEAQAVVQRAAALGVGNRLRMLGYVTDEVKYQAF
                     KVADVFVSTSQHEGFGLVFLEAMAFGLPVVCYNRGGQTDFLASPETGEVIALNDLVAF
                     NDAVRALRADPQRRALIQRANLAKVENFFIDRCAERYERLFERAVASHVTGAAARLEQ
                     D"
     gene            12223..>12980
                     /gene="asnB"
                     /locus_tag="VFB37_12255"
     CDS             12223..>12980
                     /gene="asnB"
                     /locus_tag="VFB37_12255"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZO23265.1"
                     /translation="MCGIAGIVGNTWALSEGMQRMMHALRHRGPDGEGIEQDAHAIFG
                     HRRLSIIDLEGGRQPLTNADKSIWVVCNGEIYNYQELRADLQARGYRFLTNSDCEVII
                     ALYEYYGDELLQQLRGMFGFALWDARKRRLLAARDHLGQKPFYYAQTSRGFAFASEIK
                     ALLAFDPSLRSLDLTALDQYLALRLIAPPLSMFSGVHKLPPAHSLVLEEGREPLIRRY
                     WQLDYQPKHTGSEEALLDELESRIEESLRLHLVS"
BASE COUNT         2120 a         4221 c         4283 g         2356 t
ORIGIN      
        1 tgtaaagggc ggaccccagc acgcccatgc tgcgcagcgt cgcggccggg ctgctgaaca
       61 ggctgcgcga gaagagctcg cgcagcagat gccctgccgt actgatgtcg cgtgcacgga
      121 aaaagatcca cgcgagcagg atcaacgcga aggtcccacc gatgcgcagg gcgacggtgg
      181 gcaggcgaac cagcggattg cgcgagcgca ccgcgccggc gagcgggtcg cccgtaaaga
      241 tccggggcac gacgtacagc ccgttgagca atccccagac gatgtaggtc cagttggctc
      301 cgtgccagag cccgctgagc acgaacgtca cgatgaggtt gaaggcctgg cggggacgcg
      361 aggcgcggct gccgccgagg ggaatgtaga cgtagtcgcg gaaccaggtg gatagcgaaa
      421 tgtgccagcg gcgccagaat tccaccgggc tctgcgagaa gtacggcgtg gcgaaattct
      481 gcatcagctc gaagccgaac aagcgcgccg tgcccagcgc gatgtgcgag tagccggaga
      541 agtcgctgta gatctgaaag gcgaagacca gcgagccgac cagcaccacg cttcccggaa
      601 gcgtgccgga gtgatcgaac accgtatcca ccacgacggc gcagttgtcg gcgacgacca
      661 gcttgcagaa cagcccccac aaaatctgcc tgacgccatc ggcgaaggca tcggggctga
      721 gcgtgcgcgg ccggtagaac tgcggtagca ggtgcaccgc ccgctcgatc ggcccggcca
      781 cgagctgtgg aaaaaacgcc acgtaggcga agaaggcgat cgggtcgcgt gttggttgca
      841 tctgccgtcg gtaaacggcg acggtgtagg tcagcgcctg gaaggtgtag aaactgatcc
      901 ccactggaag gataaccttc agcgtgggcg cgtgcagatg cacgcccagg cccgcgaagg
      961 cctcgatcag cgacgagatg aagaagttgt agtacttgaa gaatcccagc acgcccaggt
     1021 tgaccccgat gctgagcatg agcagcgctc gccgctgcgc gggcgcgctc acgcgaacga
     1081 ggccgagccc gacgaggtag tcgttcaggg tcgtgccgaa caacaggaac aggaatcgcc
     1141 agtcccacca tccatagaac acgtagctcg cgatgagcag ccacaggttc tgcatgcgca
     1201 gcgggccccg ctgcagcaac acgtagatga tcaggaccag gggcaggaag aaggcgtaat
     1261 cggctgagtt gaagagcatg gcgcacaatt taccccaagc ccggcactcc tgagcaagca
     1321 acgcagcaca ggggacccgt ttatgtttaa cgcgcagtgc gcgggaattg aattatcatc
     1381 ggtgtccatg gggagaccgc ggcatctgtt atgagtattc accggaatct gcgcaagctg
     1441 gggagtgagc ctttcgacgt gctggtcatc ggcgggggcg cctccggcgc ggccaccgcc
     1501 cgcgaagcgg cgctgcgcgg gttcaatacg gcgctcatcg agcggggcga tttttccacg
     1561 ggcgcctcgg cacattgctt caaagtggtg cacggtggaa ttcgttacat tcaacacggt
     1621 gacgttcccc ggctgcgcgc ctcctgcctc gagcggacga tcctgctgcg cattgcgcct
     1681 cacctggtcg caccgctgcc gttcgtcatc cccacctatg gacacggcaa gagcagccgc
     1741 tggctgctcg gcaccggcat gctgatgtat gactcgctga ctgcggacct gcgccgccag
     1801 gtcgaggacc cggcgcgccg tatccagcgc acccgcttcc tgtcgcgcag cgagacgctc
     1861 gagctgtttc cggacctcgc ctcgcagggc ctgaccggcg ctgccgtgtt cgaggatggg
     1921 cagatgtaca acccgccgcg cctggtgttg gcctttgcag ccgccgccgc ggaactgggc
     1981 gccaccgtgg ccaaccacgt cgaggccgag cggctgctgg tcgatggcgg acgggtggtt
     2041 ggcattcagg ccgtggaccg cctggagggc accgagctcg agatccgcgc gcgactggtc
     2101 atcaacgcgg cgggaccttg gtctgaaggg ttgctgcagg aatcggcgct gcagtatggc
     2161 gctcagggga cctactcgcg cgatgcctgc ttcaccgttg cgcgcaaata ctcaactccg
     2221 atggcgctgg ccgtgcaggg ccgcacgcgc gactcggatg cgctgctggc gcgcaacagc
     2281 cgccacctgt tcatggtgcc atggcgcgac tgcaccctgg tcggggtgtg gcacgtcgtc
     2341 gtgcctcgcg agccggacac tgttggattg ccgcccgagg agctgcagga gttcatcgag
     2401 gaattcaaca gcagctatcc ggctctacac ctgagcgagt cggacgtgcg ccgggtggat
     2461 ttcgggctgg tgccgttcgg ggaggcctcg cgtcagcagc aggggctgag cttcggcaag
     2521 cagtcgcggc tcatcgatca tcggcagttc ggcctggcag ggctgatctc cctcatcagc
     2581 gtgcgctaca cggtggcccg ccgcgatgcc tccgaggcgc tcgatctcgc gaccgcgcag
     2641 ctcggcccgc gctcccttgg caagggctcc gcctacctcc cgctgccggg cggggacatc
     2701 aagcgcttcg atgcgacgct tgccgatctt gctcagcggc gcccgagctg gtttcccgcg
     2761 gcggcggtcg atggtctggc gcgtaattac gggacccgcg ccgccgaggt gctctcgttg
     2821 ggtgaacgtg agccgcccct gcgccgctgc ttcaccggga cccaggtcac ccatgctgaa
     2881 gcggtccacg gcgtgcgtgc ggagacggcg cagcgcatga ccgatgtagt cttccggcgg
     2941 accgagctgg gcaccggagg gcacccgggc accgcggccc tggatgagat gcaggccctg
     3001 ctggcgcgcg agttgagctg gtccgaccgc cgcaccgtgg aagaacgcac ggcggtcgag
     3061 ctcgaattcg cacgctacct ggcctgcccg ccgcagcaag cggcccgggc gcgcagcgcg
     3121 tagatcatga ccgaggacgc gctgccgaaa atactgatca cgggcggctc gggcttcatc
     3181 ggggctcgcc tcgcacagct cgcgacccaa ctcggctacc gggtcacagt caccaccgcc
     3241 gtcaacaaca acacggagcg cgcccgttgt gatgcgctcg ccaaggcggg cattgccgtc
     3301 atcgttgcgc cgctcgaaga ggcttcgacg ctcgagcggg cgctcgccgg tcaggatgtg
     3361 gtgatccacc tcgcggcggc ccaacatgag gcacaggctc cggagagcca ctttcaccgc
     3421 gtcaacgtcg agggaacgca gcggctgctg gagatcgcct ccaggaccgg tgtgcgccgc
     3481 ttcgtgcacg gcagcaccat cggggtctac ggacaggcga gtgatggcat gctcgatgag
     3541 cgcagcccgc ttgcgcccga caatccatac gggcggacca aagctgccgc tgagcagatc
     3601 gtgcgtcagg gcgccggcaa gctggagtat tgcatcatcc ggatctcgga gacctatggt
     3661 ccgggggaca tgcgcctgct caagctgttc aggggcattc gcaagggccg cttcctcatc
     3721 gtaggtcctg ggacgaacct gcatcagctc atctacgccg atgacctggc gcggggactc
     3781 ctcgcagcct gcgcttcggg ccgggcgaac ggtgagacca tcgtactggc cggatccgag
     3841 cggatctcga ccagcgaaat ggtggccgca gtgggtgcgg ctgtgggtcg caacaagcag
     3901 ctgccgcgcg cgccgatgtg gccgttcgtg gcagcggcct cggtgctcga gtcgacgatg
     3961 acgccgctcg gcctcaagcc cccgctgcac cgtcgccgtc tggatttctt ccgcaagtcc
     4021 ttcagcttct cgaccgagaa agcgcagcag cttctcgact tccgcgccca ggtatccttc
     4081 gctgagggcg ctcgccgcac cgcacagtgg tacagcgcca acggtttcct gtgagcggcg
     4141 agagcaccct ggtgctgcct ggtgttgcag cgaaacgcag cgcgatgatc accgcgctcg
     4201 ttctgaccgt gctgtgtctc ctcgcgctcc cggagatcac cggccgaccg gtggtcaagg
     4261 atgcaggcga atcgctgcgg attgcgctcc acgtcgagca cgacggggtg tattcggaga
     4321 atccgcggcc gccctacgga cccacgatgc tgcgagagcc ggtccccatc atggtgacgg
     4381 tggctgagat ctgggcgatg gacgcggtgt tggggcgcgc agaaccggcg gcctacttct
     4441 caggcgatcg agcccgctac gtgaagctgc agaacctgct atggctcggc ctgctgatgg
     4501 ccgcggcggc cggcagcgcg gccttcttcg gcctctcggt gccttggcaa atcgccgccg
     4561 ccattgctgt cgagctcccc ttcacggtgc tcgtcccgcc gggcactcgc tatgccatcg
     4621 gcttggatag cctggctacg gagctcgcag gggccgccct gctgtgcacc ggctcgttgg
     4681 ttctgacgat cggattcgcg cgccgcagag tgcgcaccct ggcgctcgcg agcttcgtgt
     4741 ttggactgct cgccctgacc aaggcggcgt tcttctacat ctatccgctg gtgcttgcct
     4801 ccaccgcagt cctctacgcg gccgtgaccc cggcggcgcg ctggatccgc aaacagacgg
     4861 cgcttgcgct gctcgccctg gcgttgcctt tcctcgtcct gtgcagtgct tggatggtgc
     4921 gcaatcatgc gcagaccggt tatttccaga tggccgaacg gggtggtccc atcgtgctct
     4981 accgcggcct tctagatggg atgacgcatg aagagtacgc gggctccttc tatgcctggg
     5041 cggcctccgg gaccttgcaa cgcgtgtttg gcattctcac aggcttcacg ccccccgacc
     5101 tcgaagccgg cggaagattg caacatctgg ccatgggact gcccggggct gcggggcagc
     5161 gcgatctgga gaacgaggac gaaggacggc cggagctggc cctgacctgg taccgacaga
     5221 gccgggcggt ctacgagcgg tatcggcgcg cggagatcgc cgccggggcg cgctatcccc
     5281 ccggagcggc tgacagaaaa acagaggccg acggatttaa gttaatagcg ggccgccggt
     5341 ggatgcactt tgcgctcttc gtcccgctgt tgctgcgcgg cgctacggtt atattcccgt
     5401 tgctcgtcct gacactggtg tatgcacgcg cggcgcggcg cggcgagctg gccgtattca
     5461 tcctgccggc gctggggttc gtgttattct acgccatgat cacgcacttc gtgcagcgct
     5521 acggctggtt gccgcggccc gcttccacga ttgccctcat cgtcatcgtc gcggagctct
     5581 ggcggcatgg ctggccacga actgccgccg agcgtgcgtc cgctcaaccc gggtaaccga
     5641 tccgactcga accccggcgc acggatgccg ccagcaaatg taaagcacag taacgctacg
     5701 gtcactgaat catgaatccg gtcatcgccg tgctcgtccc ctgttacaac gaacaggcag
     5761 ccatcgcctc ggtggtgcgt gactttcgcg cagcgctccc cggagcggcc atcttcgtat
     5821 acgacaacaa ctccacggat cagacgcgcg ccgtggcgag cgctgcgggg gcgatcgtgc
     5881 gcagtgagcc tttgcgtggc aagggcaatg tcgtgcggcg gatgtttgcc gatatcgaag
     5941 ctgacgtgtt cgtgctcgtg gacggcgatg atacttacga cgcctccagt gctccaaagc
     6001 tcgtgcagct tctgctggcc gaatcactcg acatggtgaa cggctctcga gtcgcttcct
     6061 ccgatctggc ctatcgccgc ggccatcagt tcggcaacgc gatgctgacg gggctcgtcg
     6121 cgcgcatctt cggcaatcgt ttgaccgacc tgctctccgg ataccgcgtg ctgagccgcc
     6181 gcttcgtcaa gtccttcccc gccctgacgc cgggcttcga gatcgagacg gagatgaccg
     6241 tgcacgcgct cgagctgcgc ctgcccatcg cggaagtgcc gaccacctac cgggagcgcc
     6301 cgagcggatc ggccagcaag ctgcgcacgt atcacgacgg gattcgtatt ctgcgcacca
     6361 ttctcattct gatgaagcag gagaagccgt tgagcttctt cggtttggtc ggggcgctgc
     6421 tggccctgct ctctgcgggc ctcgcgatac cggtggtcca cacgtatctg agcacgggcc
     6481 tggtgccgcg tttgccgact gcggtgttga gcatgggcat catgctattg gcctttctga
     6541 gcttcttctg cggcctgatc ctggaaacgg tgacgcgcgg gcgggtcgag gcgaagcggc
     6601 tgcactactt gagcattccg attcgcttca cctccgtcgc aagccaggag gccgaggcgc
     6661 cttggcgcac gggctccgac agataatccg ccagttcctc gccttcggca cggtcggcgt
     6721 cgccggcttc gtggtcgatg ccggcgtgct gactttgatg ttgcgcttga cgggtgcggg
     6781 gccgtacctg gggcgagtcg tctcgttctt gtgcgccgcg acgtttacct gggcgctgaa
     6841 ccgggtcttt accttccgcg accagggcac tcatcacaca ctcggcgccc agtggctgcg
     6901 atttctgggc gccaacgcga tcggcggctt cgtgaacttc ggcgtctact cgctcttgct
     6961 cgccaccgtc gcgttcttta ccgcccatcc ggtggcaggg gtggcagccg gctcgctcgc
     7021 ggggctggcc gtcaacttca cactctcacg catgttcgtg ttctcggcga gcacacgctg
     7081 agcgctgcct gagcaccgga gcaccgtgtt aggagtgaag ggccgacccg gaaacaggcg
     7141 ttcacagccg cgagggtgaa gctggcgagc agccggatta tgttgcaaca gccgcgccgc
     7201 aaagcgccgc tgagcgctgt cggaagcgag agcggcccga gcccgaagaa tacagcgatc
     7261 gcggccatga agatcagaaa cacaagcgtg gcgtgctgca cggcacagag tgagccagac
     7321 ctgatcgcgg gagcactcgt gcgcgggcgc acggaccagc ctgctttttc gctcagctcg
     7381 gtcgcggcgc tggcagcagc gctcgctgcc ggcgaatgca catgccgcgg gtataagttc
     7441 gtgaggctct tgtgtaagat ccctgccctt caatcccttg cgcatcgaaa caccgacctt
     7501 gtccgaccct cttggcagcc tgacgcgcgc cgagcaacaa ccaacaccgc gagcggtggg
     7561 atcctctgtg gcaggcaccg ttgcgggacc ggagctcacg atcgtcatcc cgaccctcaa
     7621 cgaggagggc aacatcgagg ccctggtcgc gaagctgcac gccgttctgc gagggatcaa
     7681 ctgggaagtg atgtttgtcg atgacgactc gcgggatcgg acgcggtcgg tcacaacacg
     7741 cctggcacgg agcgatggtc gcgtccgcct gctccatcgc atcggacggc ggggactcgc
     7801 ctcggcctgc atcgagggcg tgcaggcgag cacggcaccc tacgtagccg tgatggatgc
     7861 ggacctgcaa catgacgaag cgctgttgcc caggatgttg gaggttctgc ggggcgggac
     7921 gaccgacatc gtggtcggca gccgttacgt cgccggcggc gatgtcgagg gctgggaatc
     7981 ggggcgcgcg cggatgtcca acctcgccac gcgcctggga caggtgatcc tcaggacaca
     8041 cgtcgccgat cccatgagcg gctttttcat gttgcggcgg gaagctttcg atctcaccgt
     8101 gcggcacctg tcggccgtcg gcttcaagat cctcatggac atcctcgctt cggcggagcg
     8161 gccgctgcgg gtcgaggagc tgccatatca cttccgaccc cgcgtggccg gcgagagcaa
     8221 gctcgatgcg atggtcgcgt gggaatacct catcctgctc gccgacaaga ccgtgggccg
     8281 cttcgtgccg gtgcgcttcg tgatgtttgc gatgatcggc cttctcggcc tgggtgtgca
     8341 tctgctcgtc ctgcgattgg gcctcagcct cctcgagctt cgcttcacgg cggcccaggc
     8401 gaccgcgacc ggcattgcga tggtcgccaa cttcacgctc aacaactggc tgacctaccg
     8461 cgaccggcgc ctcaaggggt ggggcttcgt tcgcggcctg ctgtcgttcg ctttcgtctg
     8521 cggtatcggc gccgcagcga atgtctccct ggcatcgttc ctgttcggac gccaggagtc
     8581 ctggtggctg gccggcatcg ccggcgccgc catgagctcc gtctggaact atgcggtcag
     8641 cgcggtactg acgtggaaag cgcgctagcc agcggcggcc ccggcacccg tgtagcgacc
     8701 gcccagccgt gggagagcga tctggcgcgc ttgctccagg cagccgtgct cggcgcgatg
     8761 gcgctcggaa tcatcgccac gatgctcggc ccgctgcacc gcatgccctt gtggatgccg
     8821 cgggatgaga acgaaggctg gaacgcatac caggcgctcg cagccctctc cggctcattg
     8881 ctctacccgc cgggcaatgc gttcatcacc aacaactacc cgccgctctc gttctacatc
     8941 gtgggagcgc tcggccgcgt gctgggtgac aatctcgtag ccggcaggct catcgcgctg
     9001 ctgagtgagc tcgcggtcgc cggcaacatc ttcctgctcg tgaggatcct gcggggtgag
     9061 cgcttctttg cgcttttctc gagcctgttg ttcctgctct acatcggcgc gaacgcccag
     9121 tcatacgtgg cgatggatga tccggaatgg ctagcgcacg ccttcgtcac cggcggcgcg
     9181 gtcctgtttc tgcgcgcgca gagcacctgg cgtccggcag tgtccctgct gctctccggc
     9241 ctgctatgtg tcgcgggagt cctcgtcaag cacaacgtga tcgtgttgcc attcgtcgta
     9301 ttcctgtggg cgctgctgaa cgatcgacgc cagctgctcg cctgggtggc gatcggcagc
     9361 atcctcggag cggtttcgct gctgctcatg accgatgcat atggtgtggt cttcctgact
     9421 gatgtcctcg gacacaagcg gggcttgtct ctccacaagc ttgccggtga cagcgtgcgt
     9481 ctgctgggcc cgatgactcc gctcttgctc gccgcagcgc ttctcggcgc cttcgcctgg
     9541 cgcgagacct cgatgcggct ggtgctgacc tatgccctcg tcggcggcgc cattggactg
     9601 tggtttctgg ccggcgtcgg catcgatgtg aatgtgctgt tcgatctgat catcgcgctg
     9661 agcgccgcgg cgggcctatt tgccaggcgc ctggtgccga tgttcgcccc ggagcatcgc
     9721 cggctggccc cggcactgat caccgcggtg atggcagcat tgtgtgcgcc gactgccatc
     9781 gaggcgaccg tgagtggcct cgatatcatt cgccaggatc ggctgcagag cgggcactat
     9841 cgcgagctca tcgcggcgat cgccgccgcg ccgggcccgg tggcctgcga gcagccctcg
     9901 ctgtgttact gggccggcaa gcccttcgag atcgacttct cgaactacgg caagaagatg
     9961 cacgccggtg tgggggggcc tgaggttctg cgccagcgct tcgatgcagg ctactatgcg
    10021 tacatccaga cgaccgggca cccggccgag gtgcgcaatc tcacgcgaca ttacttaggc
    10081 gatgcgctcg cacgccagct cgatcagcgc tacgtggccg taaggcaagt caacgaacat
    10141 tacctgctgg cgccgcgctg agcggcccac accgggctca gccgggagcg ctcgcgaccg
    10201 ccgcgacttt cttgctcagg acccgtacct tcttgccata cagcatgaag atgacgcgga
    10261 acatgaattc gagcgtggcg gtcacggcca gtcccacgta gttgagcggg ttcttccaga
    10321 acacatacag cttcagagca aaaacgcgta ccgactcgaa accggccagc ttcagaatct
    10381 gctccaggct gtactcggtg acgttgtaga agtgatcgat gttgtgtgac agattctccg
    10441 agcccacgag cgggttcgcg ccattcatcg catacaccac gatgcgcccg cccggacgca
    10501 gcgcctggcg gcacagccgc aggaaatcga cggtctcatc gagcgtcaga tggttgagct
    10561 cctgctccgg gatgatgacg tcgaactggg cctgcttgcc ctgcaggaac gggaaggcgc
    10621 gcgccgtctc gcagggaagc tggtgcgcac gagcgatctc caccttggcc ggatccgagt
    10681 cgatcccgag cacggagcga tagccgcagt cgcggagcat ctgcaccagg tagccgggcc
    10741 cgcaacttat gacaagtatg cgagcgctcc ggtcggcagg catgtgcgcc aggtagttgg
    10801 cgcgataata ggccgtgaag gagcggaagc ccttctcaac atccttcggc gcctgccagt
    10861 acgagtcgaa cggctccagg cgggccgcga gctgcggttc gggcacgccc gagcctggct
    10921 tgagcggctc cggcctaagg ttggcattca tcccgactat ccccaaatta cgcttataaa
    10981 ggtcgccctg tccgggcaga atacacggac tgggcgcccc acgggacgta cattacacat
    11041 tgcggattct gttctgtaac tacgagtatc cgcccctggg tggaggcggc ggtgtcgtca
    11101 tggccgcgat ggcgcgcacg cttgcgcgcc gccacgaagt caccgcgctc agctcgcgag
    11161 ccgccggcct gccggccgag gaactcgatg gcggcgtgcg ggtggtccgc gtacccgtgc
    11221 tcttccgccg taagctggcg gtcgccaatt ttccctccat ggcggcgtat ctgccgctgg
    11281 ggctgtggcg cggcctgcag ctcgggcgtg agcagcgctt cgatgtgatc aacactcact
    11341 ttgccgtgcc caccggtccg ctcggtgatt ggctgtcgcg acgtctcggc gtccccaacg
    11401 tgctctcggt gcatggtggg gatctttacg atccctccaa gggcagctcg ccgcaccgcc
    11461 acgccttcct gcgccgcgcc gtggcacgtc tgctcaggcg agccgatctg gtcgtggcgc
    11521 agtcacgcga cacagcccgc aacgtggcac agatctacgg ggtcgatcgc gaggtcgagc
    11581 tgatcccgct gggtatcgat cgcccgcctc agggcattca ggccggccgt gcccgtttcg
    11641 atcttcccga ggatgccttc gtgctcgtca cggcgggcag gatcgtcgcg cgcaaggcga
    11701 gcactcaact catggatacg ctcgccgctg tgccggatgc gtacttgctg gtggtgggcg
    11761 acggacccga agcgcaggcg gtggtacaac gcgccgcggc gctcggcgtc ggcaaccggc
    11821 tgcgcatgct cggctacgtc accgacgagg tgaagtacca ggccttcaag gtggccgacg
    11881 tgtttgtttc gaccagccag catgagggct tcggcctggt gttcctcgag gcgatggctt
    11941 tcggcctgcc ggtggtgtgc tacaaccgcg gcggccagac cgactttctc gccagtccgg
    12001 agaccggcga ggtgatcgcg ctcaatgacc tggtggcgtt caatgatgcc gtgcgagctt
    12061 tgcgcgcgga tccgcagcgc cgcgcgctca tccagcgggc caacctcgcc aaggtggaga
    12121 attttttcat cgaccgctgc gccgagcgct acgagcgcct cttcgagcgc gcggtcgcct
    12181 ctcacgtgac cggcgccgcg gcgcgcctgg agcaggatta gtatgtgcgg aatcgccgga
    12241 atcgtcggca atacgtgggc cctttcggaa ggcatgcaac gcatgatgca tgccctgcgg
    12301 catcgcggcc cggacggcga gggcatcgag caggacgcgc acgccatttt cggccatcgc
    12361 cggctgtcga tcatcgatct ggaagggggg cgtcagccgc tcaccaacgc ggataagtcc
    12421 atctgggtgg tctgcaacgg tgagatctac aactatcaag agctgcgcgc ggatttgcag
    12481 gcgcgcggct accgcttcct caccaacagt gactgcgaag tcatcatcgc gctctacgag
    12541 tactacggcg atgagctgct gcagcagctg cgcggcatgt tcggcttcgc gctgtgggat
    12601 gcgcgcaagc gccggctgct ggccgcccgc gatcatctcg gacagaagcc gttctactac
    12661 gctcagacca gtcgtggatt tgcgttcgcc tccgagatca aggcgctgct cgccttcgat
    12721 ccctccttgc gcagcctgga tctcacggcg ctcgatcagt acctcgcgct gcgcctcatc
    12781 gccccgccgc tgtcgatgtt cagcggcgtg cacaagctgc cgcccgccca ctccctcgtc
    12841 ctcgaggagg ggcgcgagcc gctgatccgg cgctactggc agctcgatta tcagcccaag
    12901 cacaccggct cggaggaggc gctgctcgat gagctcgagt cgcgcatcga ggagtcgctg
    12961 cggctgcacc tggtgagcga
//
LOCUS       DASRHN010000308         6728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_598520, whole genome shotgun sequence.
ACCESSION   DASRHN010000308 DASRHN010000000
VERSION     DASRHN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598520"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFB37_12260"
     CDS             complement(<1..1033)
                     /locus_tag="VFB37_12260"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZO23266.1"
                     /translation="MGSVKGSVPGSDPHKRVRLGSVEPALLAVLKQLDPLVVVITLLV
                     SALVCHVPLTPALGAVAVLAFIISSRIFGRGDREHDLAGKPLTTTFSRIALEWMSVVC
                     ILLFTAFAFKVSALFSRAMMLTWFAATPVALAAAHAVRSRARWLLTNSAYAPTYLIVG
                     ANSLGFELFRRLPRKGFLGFFDFRSPDRVAQVIDPAQLIGHCKDVAEFARANNVGSIY
                     IALPLSNVPRIGEMIRELRDTTASVYFVPDVFAFDLIQGRLVEINGMPALSVCDTPFY
                     GMDAVQKRMVDLAFASLALILLSPLMLLIALGIKLTSRGPVLFRQRRYGLNGEAIMVY
                     KFRSMTVCED"
     gene            1699..2616
                     /locus_tag="VFB37_12265"
     CDS             1699..2616
                     /locus_tag="VFB37_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HZO23267.1"
                     /translation="MLLTSVCANALAASDYVYIGGNASANAVVGQKYWFHPWLSASSP
                     SKVTFHGHNLPGWASLDSKSGSLSGTPAASAVGTYKDISITATDGVKTSTIPSITIKV
                     STSGAGKPPTTPPSSPPPAPPGNSAPQISGTPATSVVAGALYSFQPSASDANGDKLTF
                     SVKNAPSWASFSATSGKLSGTPTTANVGTTSNIIISVTDGKASASLAPFALTVGSAST
                     TTGSVSLSWTPPTENTNGTALTDLAGYRIMYGTSASSLSSSIQVSNPGLADYVVQDLA
                     PATYYFEVVAYTTTGTQSPPSAVASATIH"
     gene            2741..3400
                     /locus_tag="VFB37_12270"
     CDS             2741..3400
                     /locus_tag="VFB37_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HZO23268.1"
                     /translation="MLRFAANALAALLLLSACDGGPDVVQSTNAGAQSANPPPASSGI
                     TISGQPPTSVPAGSNYSFTPTTNATGANVSFQIRGTPSWATFNNLTGTLAGAPSSGNV
                     GTYEGIVISVSDGSSSASLAPFSIQVTANKPPGSVTLAWDPPTMNVDGTPLTNLIGYR
                     IYYGTDASALDRSVPVTDSTATSYSVQGLAAGTWYFAIQAVTAVGSESALSNLASATI
                     S"
     gene            3575..4213
                     /locus_tag="VFB37_12275"
     CDS             3575..4213
                     /locus_tag="VFB37_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HZO23269.1"
                     /translation="MRVAKLNGRQWVLLVLTCLAFATTWAQSPAGAPAQAGAASGKSS
                     VPADYLIGPGDTVEVFVWRNAELTATVPVRPDGKISTPLVEDMVAVGKTPSQLARDIE
                     KVLSEYVKSPEVNVIVTVPASVFSQVKVIGQVKNPSALPYRDGMTVLDAVLAVGGLGP
                     FAAGDRSRLVRTENGKQTEIKIKLEKLVNSGDMTQNLALRPGDVLVVPESRF"
     gene            4228..5856
                     /locus_tag="VFB37_12280"
     CDS             4228..5856
                     /locus_tag="VFB37_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HZO23270.1"
                     /translation="MSPAIEHLKEQIRGAWRFRWLGLAAAWVIALCGWVFVFSMPDRY
                     EATASVFVDTRTPLTPALQGLTVEQDVNSELNYVRQSLLAGSELEKIAQQVGVLPQTA
                     IDPVRREKLLDELRKHIQISQRSASDREDERTAGSIYDIIYQDKVQSRCLALVQDMLN
                     TFVEQTLGGKREGSVSAQQFLVVQIKDYEKRLRTAEDRLADFKTKHLGLMPSEQGGYF
                     QQLQKETDAVSDLQAKLHTAESRRATLQAQLHGDAAVAAIGSAPAMVGTSGLAAGTDT
                     VSRIAETQAHLDELLLKFTDKHPDVIAARETLEELKKRRAAEIDSLRHGDANAVAASR
                     ASANPVYQSIQLQLNQADLDVSDLKSQLGEHEEKVQALRKLVNTAPQVEAEFAQLNRD
                     YDVNKSEYTALLANLEKARLSQRADDAGSVRFEIVQSPIVSVKPVSPRRALLLAVILL
                     AAFGAGGALAYGLDQLYPVFGSSSTLTRISGVPVLGCVAPAFPRKSARLALRDGGLMV
                     AVAACLLAGFGIAVFLSHEGYRVTIAALRQLGNV"
     gene            5853..6728
                     /locus_tag="VFB37_12285"
     CDS             5853..6728
                     /locus_tag="VFB37_12285"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO23271.1"
                     /translation="MSLIENVFEKMRRAGALEADPNAARAAALSVSPLPARTEPEYPY
                     RRITVDLEMLRQQGYLPEPEQERRLADHYRQIKRPLIEAALNPAGSPDLRLILVTSPM
                     PGDGKSFTSINLAMSLARERDTTALLIDADLPKAHISRAFGVQGEPGLVDALLDETLE
                     VESLILRTNVPGLDLLPAGKWAEGVSELLTSARMAQIAARIAARNPRRLALFDSSPLL
                     ASIEARALVQIPGQIVLVVRAGKTPRQGVLDAISHLDKKKLRGLVLNDARAASGTGYY
                     DYSYYGSVDGTSRGG"
BASE COUNT         1287 a         2150 c         2136 g         1155 t
ORIGIN      
        1 cgtcttcgca gaccgtcatg ctgcgaaact tgtacaccat gatcgcttcg ccattgagcc
       61 cgtagcggcg ctggcgaaag aggaccgggc cgcgagacgt gagcttgatc ccgagcgcaa
      121 tcagcagcat cagcggtgag agcagaatga gcgcaaggct ggcgaaggcc agatccacca
      181 tgcgcttctg cacggcgtcc atcccgtaga aaggcgtatc gcataccgac agggccggca
      241 tgccgttgat ctcgaccagc cgtccctgga tcagatcgaa ggcgaagaca tcgggaacga
      301 aatagaccga ggcggtcgtg tcccgcagct cacggatcat ctctccgatg cgcggaacgt
      361 tgctcagcgg cagggcgatg taaatagatc cgacattatt ggcgcgggcg aattcggcca
      421 cgtccttgca gtgcccgatg agctgcgcgg ggtcgatcac ctgagcgacg cggtccgggc
      481 tgcggaaatc gaagaagccc agaaaccctt tgcggggcaa acggcggaag agctcgaagc
      541 cgagactatt ggctcccacg atgaggtacg tcggcgcata agcgctgttc gtgagcagcc
      601 agcgggcgcg cgagcgcacg gcatgtgctg cagccagggc caccggagtg gccgcgaacc
      661 aggtcagcat catcgcgcgc gagaagaggg cgctcacctt gaaggcgaaa gcggtgaaca
      721 gcaggatgca gacgaccgac atccactcta gcgcgatgcg tgagaaagtc gtcgtaagcg
      781 gcttgccggc cagatcatgc tcgcggtcgc cgcgcccgaa aatccggctc gagatgatga
      841 acgccagcac tgcgacggcg cccagggccg gagtcagcgg aacgtgacag acgagcgccg
      901 agacgagcag cgtgatgacc acgacgagcg gatcgagctg cttgaggact gcaagcagcg
      961 ccggctccac gctcccgagt cggacgcgct tgtgcgggtc cgatccggga accgaacctt
     1021 tgactgatcc caccacggcc gccacggttt ctctgtcctc cccttcttgc ttcttagtcg
     1081 ttgattatgg gcagatcaca ttacttcatc actacacacg ctcgcgtgca ggtgatgcgt
     1141 cgaaaactgt gaactgtgtc cgcctggcaa gctgtagacc ttttgctcac agataacagg
     1201 ccggcggaca ggaacccgcc acttcaagat ggccttcgcg gcagcggctc agcgccacga
     1261 ctgcccgctt gaacgggttc tgctccgcgg cggggcaagc gtacaccgaa tctccccgcg
     1321 gaggtgaatt atggcggttt ccacctccgg cgcctgaatt tacggctccg cgccacattc
     1381 gtgaggtgtt aagtcaaatc acgcgccggt tgtcgctaac tgcgcacgtc ggttcgcgcc
     1441 gacattttgt gacacccatc acatcgggtt ttactgcaca taacgcgctt ttttacgttc
     1501 aaacgtgaac ggtgcgcggc tcgcgacccg atcgctcgcg ccagccgctc aagcagcaca
     1561 ggcagcagca acaagagcgc gcggtctcaa tcagctcgta cggggaaggt catgcatcac
     1621 gtcgcaccgc tcgcgcgcaa gccctcattt tgctcgaacc atgtctcacc aatccgccgc
     1681 aaagccataa cagcactggt gttgttgaca tcagtctgcg cgaatgcgct ggcggcttcc
     1741 gactacgtct acatcggcgg gaacgcttcg gcgaacgcgg tcgtggggca gaaatactgg
     1801 tttcatccct ggctgtcggc ttcatcgccc tcgaaggtca ccttccatgg gcacaacctg
     1861 cctgggtggg cgagcctgga ttccaagagc ggctcgctca gcggcacacc cgcggcgagc
     1921 gccgtgggca cttacaagga catttccatc accgccacgg acggggtgaa aacgagcact
     1981 attccctcga tcaccatcaa ggtgagcacc tcgggcgcgg gcaaaccgcc cactacgccc
     2041 ccgagcagcc cgccccccgc gccgcccggt aattccgctc cccagatcag cggtacgccg
     2101 gcgacctccg tcgttgcggg tgcgctctat agttttcagc ctagcgcatc cgatgcgaat
     2161 ggagacaagc tcaccttcag cgtgaagaac gcgccgtcct gggccagctt cagtgccacg
     2221 agcggcaagc tttcgggaac gccgacgacg gcgaatgttg ggaccacatc caacatcatt
     2281 atctccgtta ccgacggcaa agcttccgct tcattggcgc ccttcgcgct gacggtcggc
     2341 agtgcttcga ccaccacggg ctccgtgtcg ctctcgtgga caccgcccac ggagaacacc
     2401 aatgggaccg cgctgaccga tctggccggc tatcgaatca tgtacggcac cagcgcgagc
     2461 tcactgagct cgagtattca ggtctcgaac ccggggctcg cagattacgt ggtgcaggac
     2521 ctggccccgg cgacgtacta ctttgaagtg gtagcctata cgacgaccgg cacgcaaagc
     2581 ccgccgtcag ccgtagccag cgctaccatc cattaacgag agcgtgcggg cggtggcgag
     2641 cggggagggt agccgctata attccttgaa aaagaagtcg aatttaactt ttcacaggcg
     2701 ttgattccca cccgggcgac gcctgacggg gaaactcagg gtgttgcgct tcgccgcaaa
     2761 tgctctcgct gcgctcttgt tgctttcggc ctgtgatggc ggcccggacg tcgtgcagtc
     2821 cacgaacgcc ggcgcgcagt cggcgaatcc gccgcctgcg agcagcggga tcacgatctc
     2881 cgggcaacct ccgacttccg tgccggcggg atccaactac agcttcactc ccactaccaa
     2941 cgcgaccggt gccaacgtga gcttccagat ccgcggcacg ccgtcctggg ccacgttcaa
     3001 caacctcacg ggcacattgg ctggcgcgcc ctcgtcgggc aacgtgggca cttacgaagg
     3061 catcgtcatt tcggtcagcg acggttcctc ctccgcctcc ctcgcgcctt tctcgattca
     3121 ggtcacggcc aataagccgc cgggaagtgt gacgcttgcg tgggatccgc cgacgatgaa
     3181 tgtcgacggt acgccactga cgaatctcat tggctatcgc atttattacg gcacggatgc
     3241 cagtgcactc gatcggtccg tcccggtgac ggacagcacc gcgaccagtt acagcgttca
     3301 gggactggca gcggggacct ggtatttcgc catccaggcc gtcaccgccg tgggcagcga
     3361 aagcgcactt tccaacttgg cgagcgcgac gatcagctga gctgcgcggc gggcctgccg
     3421 ccgcattcct ggccactgtc cgatgtgatg cagatcacag ccgcggtgca gcgtcgcgtg
     3481 cgcacccgcc gaatgtcaga atatcccgcc tggtaccgga tacgacgtga aaacaatccc
     3541 ggcaggggtc tcccctggcg gctaggatag gggaatgagg gtcgccaagc tgaacggccg
     3601 gcagtgggtg ctgctggtcc tcacctgtct cgcattcgcc acgacgtggg ctcagagccc
     3661 cgccggcgcc cctgcacagg cgggagccgc gagcggcaag tcatcggtgc ccgccgatta
     3721 cctgatcgga ccgggcgaca cggtggaagt cttcgtgtgg cgcaatgccg aactcacggc
     3781 caccgtcccc gtgcgcccgg atggcaagat ttccacgcca ctggtcgagg acatggtcgc
     3841 cgtcggcaag acgccttcgc agctcgcgcg cgacattgaa aaagtgctgt cggagtacgt
     3901 gaagtcgccc gaagtgaatg tcatcgtcac ggttccggcc agcgtcttca gtcaggtgaa
     3961 agtgatcggg caggtcaaaa atccatccgc gttgccttac cgggacggga tgacggtgct
     4021 ggatgcggtg ctcgcggtgg gcgggcttgg acctttcgcc gccggcgatc gatcccggct
     4081 ggtgcgcacc gagaacggca agcagaccga gatcaagatc aagctcgaga agctcgtcaa
     4141 cagcggtgac atgacccaga acctcgcgct gcgtccgggc gatgtgctgg tcgtccccga
     4201 gagtcgcttc tgaacaccaa tcccatcatg agcccggcca tcgaacacct gaaagagcag
     4261 atccgcggcg cctggcgctt ccgctggctg ggactcgccg cggcgtgggt cattgcgctc
     4321 tgcggctggg tgttcgtctt ctcgatgccg gatcgatacg aagccaccgc cagtgtcttc
     4381 gtcgatacgc gcaccccgct cacgccggca ctgcagggac tcaccgtcga gcaggatgtc
     4441 aactcggagc tgaactacgt gcggcagtcg ctgctggcgg gctcggagct ggaaaagatc
     4501 gcccagcagg tcggcgtgct gccgcagacc gcgatcgatc cggtccgccg cgagaaactg
     4561 ctggatgagc tgcgcaagca catccaaatt tcccagcgca gtgcgagcga ccgtgaagat
     4621 gagcgcaccg cgggcagcat ctacgacatc atctatcagg acaaggtcca atcgcgttgc
     4681 ctcgccctgg tgcaggacat gctcaacacc ttcgtcgagc agacactcgg cgggaagcgc
     4741 gagggcagcg tgagcgcgca gcagttcctc gtggtgcaga tcaaggacta cgagaagcgc
     4801 ctgcgcaccg ccgaagaccg actcgcggat ttcaagacga agcatctggg attgatgccc
     4861 tcggagcagg gcggctactt ccagcagctg cagaaggaga cggatgcggt ctccgatctg
     4921 caggcgaagc tgcacaccgc cgaaagtcgt cgcgcgacgc tgcaggcgca gctgcacggc
     4981 gatgcggccg tggcggccat cggcagcgct cctgcgatgg ttggaacctc agggttggcg
     5041 gcggggaccg ataccgtctc gcgaatcgcg gagacgcagg cccacctgga cgagctgctg
     5101 ctgaaattca ccgacaaaca tccggatgtc atcgccgcgc gagagactct ggaggagctg
     5161 aagaagcgcc gggcggcgga gatcgatagc ctgcgccacg gggatgccaa tgcggtcgcc
     5221 gccagccgcg cgagcgccaa tcccgtgtat cagagcattc agctgcagct caatcaggcg
     5281 gacctggatg tgtcggacct caagtcgcag ctcggcgagc atgaggagaa ggtccaggcg
     5341 ctgcgcaagc tcgtgaacac tgcgccccag gtcgaggccg aattcgccca gctgaatcgc
     5401 gattacgatg taaacaagtc ggaatacacc gcgctgctgg cgaatctcga aaaagcgcgg
     5461 ctgagtcaac gtgcggacga tgccggatcg gtacgcttcg aaatcgtgca gtcaccgatc
     5521 gtgagcgtca aaccggtctc gccgcgcaga gctctgctgc tggcggtgat cttgctggcg
     5581 gccttcggcg ccgggggtgc gctcgcttac gggctcgatc agctctatcc ggtcttcggc
     5641 tcgagcagca cgctgactcg catcagcggc gtccccgtgc ttggctgcgt ggcgccggct
     5701 ttcccgagga agagcgcccg gctggcgctg cgagatggtg gactgatggt ggcggtcgcg
     5761 gcctgcctgc tggcggggtt tgggattgcc gtgttcctga gtcacgaggg ttatcgcgtg
     5821 acgatagcgg ccctgcggca actgggtaac gtgtgagcct catcgagaac gtcttcgaga
     5881 agatgcggcg ggcaggagcg ctcgaggctg acccgaacgc tgcgcgggcg gccgctctgt
     5941 ccgtctcgcc gctgccggcc cggaccgagc cggagtatcc gtatcggcgc atcacggtgg
     6001 acctcgagat gctgcgccag cagggctacc tgcccgagcc ggagcaggag cgccgactcg
     6061 ccgatcacta ccgccagatc aagcgcccgc tcatcgaggc ggccctcaat ccagcgggct
     6121 cgccggacct gcgcctcatc ctggtgacga gtcccatgcc gggcgatggc aagtccttca
     6181 cgagcatcaa tctggcgatg agcctcgcac gcgagcggga cacgaccgcc ttgctcatcg
     6241 atgcggatct gcccaaggcc catatcagcc gcgcctttgg cgtgcagggt gagcccggac
     6301 tggtggatgc gctgctcgat gagacgctcg aggtcgagtc gctgattctg cgcaccaacg
     6361 tgcccgggct cgacctcctg ccggccggta aatgggccga aggggtgagc gagctgctca
     6421 ccagtgcccg gatggcacag atcgccgccc ggatcgcggc gcgcaatcca cgccgcttgg
     6481 ctctgttcga ctcctcgccg ctgctggcct cgatcgaagc gcgggcgctg gtgcagatcc
     6541 cgggtcagat cgtgctcgtg gtgcgcgcgg gcaagacccc gcgacagggc gtgctggatg
     6601 cgatctccca cctcgacaaa aagaagctca ggggcctggt tctcaacgac gcacgagcag
     6661 caagtggaac gggctactac gactattcgt actacggatc ggtcgatggg acctcccgcg
     6721 ggggttag
//
LOCUS       DASRHN010000309         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_931922, whole genome shotgun sequence.
ACCESSION   DASRHN010000309 DASRHN010000000
VERSION     DASRHN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931922"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFB37_12290"
     CDS             <1..667
                     /locus_tag="VFB37_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO23272.1"
                     /translation="PTPSVEIAGNRQRFPVHRIYCIGRNYADHAVEMGAAPAKGVPFF
                     FSKPADAIVANGAIVPYPSRTEDLQHEIELVVAIGRPGSDIRATEARAHIFGYAVGND
                     LTRRDLQGQAKKKGLPWDAGKGFDASAPVSAIRPVSQGHFERGRIWLSVNGELRQHSD
                     VSEMMWSVPEILAELSGYFELRAGDLIFTGTPAGVGPLKPGDRLEGGVEGLDVLRNTI
                     GAR"
     gene            664..1308
                     /gene="maiA"
                     /locus_tag="VFB37_12295"
     CDS             664..1308
                     /gene="maiA"
                     /locus_tag="VFB37_12295"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HZO23273.1"
                     /translation="MKLYTYFRSSAAFRTRIALNLKGIQYERAPVDLRAPASEQRATT
                     FLALNPQGLVPTLETDGQIIRQSLAIIEYLDETHPNPPLLPRTPLERARVRAMALTIA
                     CDIHPLNNLRVLNYLRSPLRQDADVVDTWCRHWISEGFRALEQEVQSTSGDGQHVFGT
                     AVTLADICIVPQMYNARRVKCDVQPYPTLVAICAHLEKLPAFAKAAPEAQPEAG"
     gene            complement(1315..>2109)
                     /locus_tag="VFB37_12300"
     CDS             complement(1315..>2109)
                     /locus_tag="VFB37_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZO23274.1"
                     /translation="QVAEALERLAQRESRTLLPGYTHMQQAMPSSVLLWAGGFAAEIR
                     DDAEGLLLAQRRVDRNPLGSAAGYGTPGLPIDREATRAKLDFKTTQEPVSAVQLSRGK
                     AESHVLFEIVMLMQDLARFAADVLLFYTAEFGFIELSEAFTTGSSIMPQKRNPDVFEL
                     IRGRSMTALGCLLEALGICAKLPSGYQRDLQLLKFPLFRSIDLALQTLHILPSAVEAL
                     SFHSERIRLDPAIHAAEEANRLVVQEGIPFREAYRRVAARLKGNKN"
BASE COUNT          397 a          666 c          673 g          373 t
ORIGIN      
        1 tcccacgccc agcgtcgaga tcgccgggaa ccgccagcgc ttccccgtcc atcgcatcta
       61 ttgcatcggt cgcaactatg cggaccacgc tgtggagatg ggtgccgcgc cggccaaggg
      121 cgtgccgttt ttcttcagca agccggccga tgcaatcgtc gcgaatggtg ccatcgtgcc
      181 atacccctcg cgcaccgagg acctgcagca cgagatcgag ctggtcgttg cgatcgggcg
      241 ccccggcagc gacattcgcg cgaccgaggc ccgagcgcac atcttcggct acgcggtcgg
      301 gaatgatttg acgcgacgcg atctgcaggg gcaggcaaag aagaaggggc tgccatggga
      361 cgcgggaaaa ggtttcgatg cctcggcgcc ggtgtctgca atccggcccg tctcccaggg
      421 gcacttcgag cgcgggcgaa tctggctgag cgtcaacggc gagctgcggc agcactcgga
      481 tgtcagtgag atgatgtgga gtgtgccgga gatcctcgcg gagctctcag ggtacttcga
      541 gctgcgagcc ggtgatctga tcttcaccgg cactccggca ggggtcgggc cgctcaagcc
      601 gggggatcgc ctggagggcg gggtcgaagg tctggacgtt ctacgcaaca cgattggtgc
      661 gcgatgaaac tgtacacata tttccgtagc tccgcggcgt ttcgtacgcg gatcgccctg
      721 aacctgaaag gcattcagta tgagagggcg ccggtcgacc tgcgtgcacc cgcaagcgaa
      781 caacgtgcga ccacctttct tgcactcaat ccgcagggat tggtgccgac gctggagact
      841 gacggtcaga tcatccgcca gtctctggcg atcatcgagt atctcgacga aacgcatccc
      901 aaccctccgt tgctgccccg cactccgctg gagcgcgcac gcgtacgcgc catggctctc
      961 acgatcgctt gtgacatcca tccactcaac aacctgcgcg tgctcaacta tctgcgctcc
     1021 ccactgcgac aggatgcgga cgtggtggat acctggtgtc gtcactggat ttctgaaggg
     1081 ttccgggctt tggagcagga agtccaatct acgagcggcg atggccagca cgtgttcgga
     1141 accgccgtga cacttgcaga tatctgcatc gttccgcaga tgtacaacgc ccggcgagtc
     1201 aaatgcgacg ttcaacccta tcccacactg gtagcgatct gcgcacacct ggagaagctg
     1261 ccagccttcg cgaaagccgc tcccgaggcg cagccggagg caggttaggt cgcgctagtt
     1321 cttattgccc ttgagccgtg ccgctacgcg ccggtaggcc tcacgaaacg gaattccctc
     1381 ctgcaccacc agccgattgg cctcttctgc agcgtgaatg gccggatcga gtcggatgcg
     1441 ctcgctgtga aaactcagcg cctcgacagc ggagggcagg atgtgcaggg tctgcagcgc
     1501 caggtcgatg ctgcgaaaca gcggaaactt gaggagctgc agatcgcgct gatagccgga
     1561 gggcaacttg gcgcagatac cgagcgcctc gagcaggcaa ccgagcgcag tcatggagcg
     1621 tccgcggatc agctcgaaca catcgggatt gcgtttctgg ggcatgatcg aggagccggt
     1681 ggtgaaggcc tccgaaagct cgatgaagcc gaactccgcg gtatagaaga gcaatacgtc
     1741 ggcggcgaag cgcgccaggt cctgcatcag catgacgatc tcgaacagga cgtgcgactc
     1801 tgccttgccg cgcgagagct gcacggccga gaccggctcc tgagtcgtct tgaaatcgag
     1861 cttcgcccgc gtcgcctcgc gatcgatcgg caggcccggt gtgccatacc ccgcggccga
     1921 gccgagcggg ttgcggtcga cccggcgctg cgccagcagc agtccttccg catcatcgcg
     1981 gatctccgcg gcaaagcctc ctgcccacag aagcaccgag ctcggcatgg cctgctgcat
     2041 gtgcgtgtag ccgggcagaa gcgtgcggct ttcacgctgc gcaagtcgct cgagcgcctc
     2101 tgccacctg
//
LOCUS       DASRHN010000310         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_774664, whole genome shotgun sequence.
ACCESSION   DASRHN010000310 DASRHN010000000
VERSION     DASRHN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774664"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..>2243
                     /locus_tag="VFB37_12305"
     CDS             8..>2243
                     /locus_tag="VFB37_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO23275.1"
                     /translation="MTPLSESYKASVGAATLHETAHDFDITLDCIGDAVLATDAKGRI
                     TRMNPVAKTLTGWTRLEAQGRPVDEVFRIINEETRQPALVPVDEVLRTGEIRGLANHT
                     LLISRDGSERAIADSAAPIRDEQGIVRGVILVFRDVSQQRAAERALQESEARYRRFIE
                     LSPCGVFVQCEGRFAFLNPKALALLGASAERDLLGREVLDFIHPDNREVARERLRRLN
                     SESIGAPAREAKWLRLDGSVLHIESTAVPYQHEGRPGALVLLQDITARKVAEEQRDRF
                     FDVSLDLLCIAGMDGYFKRINPAFSQTLGWSDEELIARPFVDFVHPDDMAATLLEVQK
                     LAAGQRTLHFENRYRCKDGSWRWLAWRTMPQPDGLLYASARDITEQREAEKEMRRLNA
                     DLELRVEERTAALAALHSKEQEIRAIVDNMLDCIITLDADGVVRSVNPALQRCLGYAA
                     HEVLGRNISLLMPEAQRNNHDACFEDYLRSSEARIVGFSQEVNGRHKDGRLIPLELSF
                     SEYAVHGERLFIITLRDIRERKQFIAELSRARADAEQASRAKSAFLATMSHEIRTPMN
                     GVLGMAEVLARTTLSEHQAELVGTIRESASSLLGIIDDVLDFSKIEAGRLELERAPIC
                     VADLVEGLCNSLVPVAARRGVDLSLFISPQIPERVIADDLRLRQVLFNLVGNAVKFSG
                     GRPHQRGRVAVRVEIAQSAPLHLAFRVADNGIGMAAETLNNLFTPFTQAEVSTTRRFG
                     GTGLG"
BASE COUNT          418 a          703 c          724 g          398 t
ORIGIN      
        1 gcaggatatg acgcctcttt ccgagtcgta caaggcaagc gtgggcgccg cgacgctgca
       61 tgagaccgcg catgatttcg acattacgct cgactgcatc ggcgatgcgg tgctggccac
      121 ggatgcgaag ggacgcatca cccgtatgaa tcccgttgcc aaaacactca ccggctggac
      181 tcggttggag gcacagggcc ggccggtaga cgaggtgttt cgcatcatca atgaagagac
      241 ccgccagccc gcactcgtcc cggtcgacga ggtgctgcgc acgggtgaaa tccgcggcct
      301 agccaaccat accctgctca tcagtcgcga cggcagcgag cgcgccatcg ctgacagcgc
      361 tgcgccgatc cgcgatgagc agggcatcgt gcgcggggtg atattggtgt ttagggacgt
      421 gagccagcag cgcgcggccg agcgcgcgct gcaggagagc gaggcgcgtt accgccggtt
      481 catcgagttg tctccgtgcg gagtcttcgt gcagtgcgag gggcggtttg ctttcctgaa
      541 cccgaaggca ctggccctgc ttggagcgag tgcggagcgc gacttgctcg ggcgcgaggt
      601 actggatttc atacatcccg acaaccgtga agtggcgcgc gagcgcctgc gccgcctgaa
      661 cagcgaaagc atcggtgccc ccgcgcggga ggcgaaatgg ctgcggcttg acgggtcggt
      721 gctccacatc gagtccacgg ccgtgcccta ccaacacgaa ggccgcccgg gtgcgttagt
      781 gctgttgcaa gacattacgg cgcgcaaggt ggccgaagag cagcgcgatc ggttcttcga
      841 tgtgtcattg gacctgctct gcatcgccgg catggatggg tatttcaagc gcatcaatcc
      901 tgccttcagc cagaccctgg gttggagcga cgaggaactc atcgcgcggc cgttcgttga
      961 tttcgtccat cccgatgaca tggctgccac gctgctcgag gtgcaaaagc tcgcggccgg
     1021 tcagcgaacg ctgcacttcg agaaccgcta tcgatgcaag gatggctcct ggcgttggct
     1081 cgcgtggaga accatgcctc agcccgatgg cctgctctac gccagcgcgc gcgacattac
     1141 cgagcagcgc gaagccgaaa aggagatgcg ccgcctcaat gcagacctcg agctccgggt
     1201 cgaagagcgc actgccgcgc tcgcggccct gcatagcaag gagcaggaaa tccgcgccat
     1261 cgtggacaac atgctcgact gcattatcac gcttgatgcc gacggtgtcg tacgcagcgt
     1321 caatccggcg ctgcagcggt gcttgggcta cgcggcgcat gaagtcctgg gccgcaacat
     1381 ctcgctgctt atgcccgagg ctcagcgcaa caaccacgat gcctgtttcg aagattactt
     1441 gcgtagcagc gaagcgcgca tcgtcggctt cagccaagag gtgaatggcc ggcacaagga
     1501 cggccggctg atcccgctgg agctgtcctt cagcgaatat gccgtgcacg gcgaacgtct
     1561 tttcatcatc acgctgcgcg acatccgcga acgcaagcag ttcatcgcag aactgagccg
     1621 ggctcgggca gacgcggaac aggcgagtcg cgccaagtcg gccttcctgg cgaccatgag
     1681 ccacgagatc cgcactccca tgaacggcgt cctcggaatg gcggaggtgc tcgcaagaac
     1741 taccctgtcc gagcaccagg ccgagctggt cggaacgatt agggagtctg cctcatccct
     1801 gctaggcatc atcgacgacg tgttggattt ctccaagatc gaggccggcc gactggagct
     1861 cgagcgtgca ccaatctgcg tggccgatct cgtcgaaggg ttgtgcaatt cgctggtccc
     1921 tgtcgcagcc cgccgaggcg tcgacctctc cttgttcatc tcgccgcaga ttccggaacg
     1981 ggtgatcgcc gatgatttgc gactgcgcca ggtgctcttc aacttggtgg gcaatgccgt
     2041 caagttcagc ggcggccggc cgcatcagcg gggccgggtc gcggtgcgag tagagatcgc
     2101 ccagagcgcg ccgctgcacc tggcatttcg cgtcgccgat aacgggatcg gcatggcagc
     2161 tgagacgctc aacaacctct tcactccctt cacccaggcc gaggtctcca ccacgcggcg
     2221 cttcggcgga accgggctgg ggc
//
LOCUS       DASRHN010000311         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_270859, whole genome shotgun sequence.
ACCESSION   DASRHN010000311 DASRHN010000000
VERSION     DASRHN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270859"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VFB37_12310"
     CDS             complement(<1..1427)
                     /locus_tag="VFB37_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO23276.1"
                     /translation="MIRAAAAALLAAAVALVLLITACAHQTDKGGTLASLRGVRADTS
                     EAPVTQGLDKAMESYAKFLQQNPDSKLTPEAMRRLADLKIEKEYGIQGDGKLIDVPPG
                     KPTAQADTAKTTQAPRSAQALSAIAAPTPGRIDEHSAERARRAAIAPALPTDSERDLE
                     KRAMAQQALPGAPLPPNAQLPEGVNHDLDRAGPLEAIKLYDELLAKYPSYPFRDQVLY
                     QKARAYDELGRTEESMQVMEELVGADPHSRFADEVQFRRAEHFFIRRKFRDAERAYTA
                     IVQKGQASEYYELALYKLGWALYKQEFYEEALHQYLALLDYKVSSGYDFDAKHEEPEQ
                     RRVEDTFQVVSLSFSYLGGPEVIGEYFASNGHKGYEDRVYRYLGEFYLTKLRYQDAAK
                     VYNGFVALYPFHAAAPRFSMRTIEIYEKGGFPKLVLDSKKQFASQYGLHAGYWTHFDV
                     SKSPEVLSYLKSNLTDLANHYHAQY"
     gene            complement(1424..>2449)
                     /locus_tag="VFB37_12315"
     CDS             complement(1424..>2449)
                     /locus_tag="VFB37_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23277.1"
                     /translation="ATDSAVQEAKLALPYAYSKLQVYGRAAQLYGQALDSFGKELEKV
                     DASIRSIRAGRFLQALVREEIEQDRQWVVRLRSLPGTPETFYLTELMASHDFQTALQN
                     YLDLEDLRKRLIVWQNGFDAFDDIIRLRRQNYAPLLPKIDAQFKDLDAQVRLRVEQRD
                     HLQKRLREILTSPRPELLATSDERIAAERIHRLEGALANSTGADAAALRERVQHLKGV
                     LTWDLYTQFPERLTEAYAHLAQLNENVKVMRERYDSFVRARQAAVHSYEGYDPSINRL
                     RTRVHDGLDRVNTLLAQQGQLIETVAISELKTRGERLEAYQTQARYAVADSYDRATKA
                     QGGGGQP"
BASE COUNT          393 a          790 c          754 g          512 t
ORIGIN      
        1 tgatactgcg cgtggtagtg gttggccaga tccgtcagat tgctcttgag ataactgagc
       61 acctcgggcg atttgctcac gtcgaagtga gtccaatacc cggcgtgcag gccgtactgg
      121 ctggcaaact gtttcttgga gtccaggacc agcttcggga agccgccctt ctcgtagatc
      181 tcgatcgtgc gcatgctgaa gcgcggcgcc gccgcgtgga acgggtacag cgcgacgaac
      241 ccgttgtaaa ccttggctgc atcctggtag cgcagcttgg tcaggtagaa ctcacccaga
      301 taccggtaaa cgcggtcttc gtaacctttg tggccgttgg aggcgaaata ctctccgatc
      361 acctccggac ccccgaggta ggagaaactc aggctgacga cctggaaggt atcctcgacc
      421 cggcgctgct ccggctcctc gtgctttgca tcgaagtcgt agccgctgga gaccttgtag
      481 tccagcagcg cgagatactg gtgcagcgcc tcctcataga actcctgttt gtaaagcgcc
      541 cagccgagct tgtagagagc cagctcgtag tactcgctgg cctgtccttt ctgcacgatc
      601 gccgtatagg cgcgctccgc gtcccggaat ttccgcctga tgaagaagtg ctccgcccgc
      661 ctgaactgca cctcgtcggc gaaccgcgag tgcgggtccg ctcccacgag ctcctccatg
      721 acctgcatcg attcctcggt ccgcccgagc tcgtcgtaag ccctggcctt ctgatagagc
      781 acctgatccc ggaacggata ggacgggtac ttcgcgagca actcgtcgta caacttgatc
      841 gcctcgagcg gtccagcgcg atccaggtcg tggttgacac cctcaggcaa ctgcgcgttc
      901 ggcggcaggg gtgcaccggg aagcgcctgc tgcgccatgg ctcgtttttc gagatcgcgc
      961 tcggaatccg tgggcagcgc cggggcaatg gctgcccgcc gcgcgcgctc tgcggaatgt
     1021 tcgtcgattc tcccgggcgt cggtgcagcg attgccgaca acgcttgcgc gcttcgcggc
     1081 gcctgcgtcg ttttagccgt atcggcctgc gctgtcggct tgccgggcgg cacgtcgatc
     1141 agcttgccgt cgccttgtat gccgtactcc ttctcgatct tcagatccgc cagccgccgc
     1201 atggcttccg gagtcaactt gctgtccgga ttctgctgga ggaacttcgc gtagctttcc
     1261 atggccttgt cgagtccctg cgtgacaggg gcctcgctgg tatccgcgcg taccccgcgc
     1321 aggctcgcca gcgtgccgcc cttgtcggtc tggtgagcac aggcggtgat cagcaggaca
     1381 agcgcaactg ctgcggcgag aagagctgcg gcggcggctc gtatcacggc tgccctcccc
     1441 cgccctgcgc cttcgtcgcc ctgtcataac tgtcagccac ggcgtaccga gcttgcgtct
     1501 gataggcctc gagtcgctcg ccgcgcgtct tcaattcgct gatggcaacc gtctcgatca
     1561 actgtccttg ctgtgccagt aatgtgttca cccgatcgag gccgtcatgc acgcgagtgc
     1621 gaagcctgtt gatcgacggg tcataaccct catagctgtg gaccgccgcc tgccgggctc
     1681 tgacgaacga gtcgtatcgc tcgcgcatga ccttcacgtt ttcattcagc tgggcgagat
     1741 gcgcataggc ctctgtcagc cgttccggga attgggtgta aagatcccag gtcaacactc
     1801 ccttgaggtg ctgaacccgt tcccgcaggg ccgctgcatc cgctccggtg gagtttgcca
     1861 acgccccctc cagtcgatga atgcgctctg ccgcgatgcg ctcatccgag gtcgccagca
     1921 gctccggtcg gggggacgtc aggatctccc ggaggcgctt ctgcaggtga tcgcgctgtt
     1981 cgacccgcag gcggacctga gcgtccaggt ctttgaactg agcgtcgatc ttcggcagca
     2041 gcggtgcgta gttctggcgg cgcagccgga tgatgtcgtc gaacgcgtcg aagccgttct
     2101 gccagacgat gagccgtttg cgcaggtctt cgaggtccag atagttctgc agcgcggtct
     2161 ggaaatcgtg cgaggccatc agctccgtca gatagaacgt ctccggtgtg ccgggcaggc
     2221 tgcgcagccg cacgacccac tgccggtcct gctcgatttc ctcccgcacc agggcctgga
     2281 gaaaccttcc ggcgcggatg ctgcggatcg aggcgtcgac tttctccagc tccttgccga
     2341 aagagtccag cgcctggcca taaagctgcg ccgcgcggcc gtacacctgc agcttgctgt
     2401 aggcgtaagg cagagcaagc ttggcctcct gtacggcgct gtcggtcgc
//
LOCUS       DASRHN010000312         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_107522, whole genome shotgun sequence.
ACCESSION   DASRHN010000312 DASRHN010000000
VERSION     DASRHN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107522"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFB37_12320"
     CDS             <1..496
                     /locus_tag="VFB37_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23278.1"
                     /translation="LRKEIGAEVDRLRGRAGPAGEADAVPWDKLTEAQRDAEARNTGA
                     EDHAGIGDSPVPYYWYERILQLAREHHVRVIGMRFPVHHEYAAQAPPQQVAAIDAFLL
                     RHGVAQIIDLRGALTDPRDFQDEDHVNDTGVVPLLPIVEKRLGRPLILHRPEQAAALS
                     SASR"
     gene            complement(527..1873)
                     /locus_tag="VFB37_12325"
     CDS             complement(527..1873)
                     /locus_tag="VFB37_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013358747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZO23279.1"
                     /translation="MNQTGKRKARILVVDDDPGLLRLLTIRLRAESYDVEAVESGAQA
                     LAAASRFRPDLVITDLRMDQMDGIGLLKELQNRWPGLKVIILTAHGTIPDAVQATQMG
                     AFGFLTKPVDKQELLDQVQKALRISGFGASDEDWRAEIVTRSSVMEEKLAQAHMVAGT
                     DARVLITGESGTGKELLARAIHHASPRKNKPFVAINCSAMAENLLESELFGHEKGSFT
                     GATRAHRGLFMAADGGTLMLDEIGDMPMRLQVKLLRVLQENVIRPVGSTDAIPVNVRV
                     ISATHRDLQGLMTAGQFREDLYYRLNVVHIEMPSLTRRREDIPLLVAHFLAQIAKESG
                     VRKIYAPEAVELLATADWPGNIRQLQNVVRQNVALSQTPIIPVELVQQSLGGGPGRLP
                     SFDEARDEFTRSYLSQILQITAGNVSQAARLAKRNRTDFYKLLARHQLTPEEFKQR"
     gene            complement(1921..2580)
                     /locus_tag="VFB37_12330"
     CDS             complement(1921..2580)
                     /locus_tag="VFB37_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23280.1"
                     /translation="MPLDCVAARAATRSRLAALAAATALLGACASDNNTHDHDQGQAT
                     PVVDHNAASAAAVTEDLQLMQRLVQGAPAEQAEISAGAQRDFDTAPTPSRELRLALVL
                     ATPGHPGTNPARAQTLLRELMANPEMLLSGERALAFLELQQIGDHLTLEAENRRLQGD
                     AVRADREHQSSANRRLQAESDENTRLRKELEEARAKLDAIANIERSLNERKPNNEGRP
                     P"
     gene            complement(2570..>2765)
                     /locus_tag="VFB37_12335"
     CDS             complement(2570..>2765)
                     /locus_tag="VFB37_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO23281.1"
                     /translation="SKNTAIKGTGIGLSVVLEFVSAHGGTVEIRDGEFPGAHFRISMP
                     VQAGGDRPVNTERRAQAHAA"
BASE COUNT          422 a          922 c          926 g          495 t
ORIGIN      
        1 cctgcgcaag gagatcggtg cggaggtcga ccggctgcga ggccgcgccg gccccgctgg
       61 cgaggctgac gccgtcccct gggacaagct caccgaggcg cagcgcgatg ccgaggcccg
      121 caacaccggc gctgaggatc atgccggcat cggggattca cccgtgccgt actattggta
      181 tgagcggatt ctgcagctcg cccgcgagca tcacgtgcgc gtcatcggaa tgcgatttcc
      241 ggtgcaccac gagtacgcag cccaggcacc gccccaacag gttgcggcga tcgatgcatt
      301 cctgctgcgc catggggtcg cgcagatcat cgatctgcgt ggggcgctga ccgatccgcg
      361 cgacttccag gacgaagacc acgtgaacga caccggtgtc gtgccgctgc tgccgatcgt
      421 ggagaagcgc ctcgggcggc ccctgatcct gcaccggccc gagcaggcgg cagcgctgag
      481 ttcggcctcg cgctgagccg cccggagccg ggacgccgag ctcgcctcag cgctgcttga
      541 actcttccgg cgtcagctgg tgccgggcga gcagcttgta gaaatcggtg cgattgcgct
      601 tggccaggcg tgccgcctgg ctcacattgc ccgcagtgat ctgcaggatc tgcgacaggt
      661 agctgcgggt gaactcatcg cgcgcctcat cgaaagaggg caggcggccc ggaccgccgc
      721 cgagcgattg ttgcaccagc tcgaccggga tgatcggcgt ctgggagagg gcgacgttct
      781 gccgcaccac attctgcagc tggcggatgt tgcccggcca gtcggcggtc gcgagcagct
      841 cgaccgcttc gggcgcgtag atcttgcgca cgccgctttc tttcgcgatc tgcgccagga
      901 aatgcgcgac cagaagaggg atgtcttcgc ggcggcgcgt cagggacggc atttcgatgt
      961 gcaccacatt gagccggtag tacaggtcct cacggaactg acccgcggtc atcagccctt
     1021 gaagatcgcg atgcgtggcg gagatgacgc gcacgttgac cgggatcgca tcggtgctgc
     1081 caacggggcg gatcacgttc tcctgcagca cgcgcagcag cttcacctgc agccgcatcg
     1141 gcatgtcgcc gatctcatcg agcatcaggg tgccgccatc ggccgccatg aacaggccgc
     1201 gatgcgcgcg ggtggcgccg gtgaaggagc ccttctcgtg cccgaacagc tccgactcga
     1261 gcaggttctc ggccatggcg ctgcagttga tggccacgaa gggcttgttc ttgcgcgggc
     1321 tcgcgtggtg aatggcgcgt gccagcagct ccttgcccgt gccgctctcg cccgtgatga
     1381 gcacgcgcgc atcggtgccg gccaccatgt gggcctgcgc gagcttctcc tccatgaccg
     1441 agctgcgcgt gacgatctcg gcgcgccagt cctcatcgga ggcgccaaag cccgagatgc
     1501 gcagcgcctt ctgcacctga tcgagcagct cctgcttgtc caccggcttg gtgagaaagc
     1561 cgaacgcgcc catctgggtg gcctgcaccg catcgggaat cgtgccatgt gcggtcagga
     1621 tgatgacctt cagccccggc cagcggttct gcaactcctt gagcagtccg atcccgtcca
     1681 tctgatccat gcgcagatcg gtgatcacca ggtccggacg gaagcggctc gccgccgcca
     1741 gcgcctgggc gccactctcg acggcctcga catcgtagct ctcggcgcgc aaccggatcg
     1801 tcagcagccg cagcagtccc gggtcatcgt ccaccacgag gatgcgggct ttgcgttttc
     1861 cggtctgatt caagtgcttc gtccttaaca actcaggttg caacagcgtc gcgggaatgg
     1921 tcacggtggt cgtccttcgt tgttgggttt gcgttcgttc agggagcgtt cgatgttggc
     1981 gatcgcatcg agcttcgcgc gagcttcctc gagctccttg cgcagccggg tgttctcgtc
     2041 cgattcggcc tgcagtcgcc ggttggcact gctctgatgc tcgcggtcgg cacgcaccgc
     2101 atcaccctgc agcctgcggt tttccgcctc cagcgtcaaa tggtcgccaa tttgctgcag
     2161 ttccagaaaa gcaagggcgc gctccccgga aagcaacatt tcagggttcg ccatcagctc
     2221 gcgcagcagg gtctgcgcgc gcgccgggtt cgtgccggga tggcccggag tcgcgagtac
     2281 cagcgcaagg cgcagctcgc ggcttggagt gggcgccgtg tcgaaatcac gctgcgcacc
     2341 ggcgctgatc tcggcctgct cggccggcgc accctgcacc aggcgctgca tgagctgcag
     2401 atcctcggtc acggcggcgg cggaggccgc gttgtgatcg accacgggcg tcgcctgccc
     2461 ctgatcgtga tcgtgggtat tgttgtcgct cgcgcacgcg cccagcagcg ccgtggccgc
     2521 agcgagcgcg gccagtctcg atctcgtggc ggctcgggcc gcgacgcagt caagcggcat
     2581 gcgcttgcgc cctgcgctcg gtgttgaccg gccggtcacc gcctgcctgc acgggcatgc
     2641 tgatgcggaa gtgagcaccg ggaaactctc cgtcgcggat ctccaccgtg ccgccatgcg
     2701 cggagacgaa ctcgagcacg accgacaggc cgatgccggt gcccttgatg gccgtgtttt
     2761 tcgag
//
LOCUS       DASRHN010000313         6559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2249728, whole genome shotgun sequence.
ACCESSION   DASRHN010000313 DASRHN010000000
VERSION     DASRHN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249728"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFB37_12340"
     CDS             complement(<1..705)
                     /locus_tag="VFB37_12340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO23282.1"
                     /translation="MDFALTEEQELIIRTTREFVRKELYPHEQQIELTGELPAAVRQE
                     LRRKAIDAGLFAANMPVEVGGAGLDGVSWILYEKELGKTSYALHYSCVARPSNILLAC
                     EGAQRERYLLPAVGGERTECLAMTEPEAGSDLRGMKTTAVRDGESFVINGTKHFISHA
                     DHSDFVILFAASGEEDSPRGKRKLITAFLVDMGTPGFVVRPGYRNVSHRGYTNSILEF
                     HDCRVPQSAVLGALHKG"
     gene            complement(731..1396)
                     /locus_tag="VFB37_12345"
     CDS             complement(731..1396)
                     /locus_tag="VFB37_12345"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZO23283.1"
                     /translation="MPKIVDHEHRRDQIALVACRVVAEHGFDQATIVRIAREAGYTTG
                     MVAHYFDTKQDIIIAALRLILRRIEERLTRPAGTAADLLTVLAEALPIDEQRYIECAF
                     WTAFWGQVSTDKRLKRINAWVHREYMRLFERCLAEGWPHWPQLASGVREQTLRSVVTF
                     INGVTASAVASQSDWPAARQLEQLRLQLQLLHGWALDSMATPVGEKHRKSHKAPAPGA
                     SAA"
     gene            complement(1425..2924)
                     /locus_tag="VFB37_12350"
     CDS             complement(1425..2924)
                     /locus_tag="VFB37_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZO23284.1"
                     /translation="MRQVKKLGLLGTGVIGGGWAARALHFGIDVVAADVKPEMEDWIR
                     GAVRNAEPALARLTLAPLPPKGKLTFTTDLCAMAREVDFIQENIPEQLELKQRVLGEV
                     SQEAPPDVVIASSTSGLMPTDLQRAMRAPERFLVAHPFNPVYLLPLVELVGGERTAAD
                     AIDAASRFYAHIGMHPLKVRREVPGHLTDRLQEALWREILHLVNDGTATTGELDESII
                     YGPGLRWAAMGTNLIYHLAGGEPGMRHMLRQFGPCLKWPWTRLEAPELTEQLIDRMVE
                     GTQAQARGRSIRELERLRDDCLVDIQQVLRRHDVGAGATLRALEERLYEENARATGAR
                     ISDQAGGSIDAPLKLIEVQVRPEWLDYNGHMTDSRYLQVFGDATDALFRQVGVNDQYR
                     KSGRALYAVESHMTHTAEAKVFDTLSVNTQLLSVDEKRVHLFHRMHRASDEVVVATAE
                     QIYLHVDAAARKASSMDASVHARLDAVRAAHASLPAPAQKGRHIGMPRN"
     gene            3022..4452
                     /locus_tag="VFB37_12355"
     CDS             3022..4452
                     /locus_tag="VFB37_12355"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZO23285.1"
                     /translation="MRGGALLETRSIDYVPLAERHGKVRDLWPVWFSGDAHLATLAVG
                     VIGIALGGNLLWTSVAVLAGCALGTFFMAFHSTQGPQLGLPQMIQSRPQFGYVGALLV
                     WGVALIAYIGYNAFNQVLAAQTVHQLWKPIQPASPTVLIVFALVALVPAAIGYDIIHL
                     AQRVFAYLMIAILGVFSIAALFVLRLPPGQWDPAAFHGGPFIAEFFAAASYQLSWSIY
                     VSDYSRYLPRSVGVRESFWWTYLGAFIGGSWMMLLGTLAAAAAPGLEVAASLQVTGNA
                     ILPGLGEVLLAGALLGLVTISSLNFYGASLTLLSVADTFKPLRSTALKRFASLLATGI
                     AGTAIAFGASGNFLTAFGDLLSVLLYLFTPWTAINLVDFYLVRRGHYSIREIFNPHGM
                     YGRWNWRGLLAYAIGFVAMIPFFSTELYRGPIARALGGADIAMLIGLPVAAVVYLFAC
                     RSLDAARDWQLASAADVGLDPGPERL"
     gene            complement(4508..5191)
                     /locus_tag="VFB37_12360"
     CDS             complement(4508..5191)
                     /locus_tag="VFB37_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZO23286.1"
                     /translation="MKDTARRSRLGQTLRALRKQRGWTLAEVSRRTGFSIPTLSKVEN
                     DRLSLTYDKLIRLSEGVEIDIAQLFAPVNGVGGPAAITGRRSVNRRTEGELVATRNYD
                     YRYLNTDVTRKKFIPIHTELHARSLEEFGELVRHTGEEFIYVLEGQVELHTELYAPVV
                     LSAGESAYIDSTMGHAYVVRGEGPCRLLAVCSASERDLREVTGGQASEVTPRASPRLQ
                     HRRRPARRR"
     gene            5512..>6559
                     /locus_tag="VFB37_12365"
     CDS             5512..>6559
                     /locus_tag="VFB37_12365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZO23287.1"
                     /translation="MEVLARALNRWILPAATSIPIWLSSAPVLAQQASASDQPATLEE
                     VIVTAQKRSEPLANVPQSITVVSSDILQRQQAFDLQDYLALIPGLSLTGDTPGQSRIT
                     LRGINSDGVASTVAVYVDDVPFGSSSGLANGAILAGDFDTSDVARLEVLRGPQGTLYG
                     ASSLGGVLKFVTNAPKTDAFDANLNVGAEDLNGGGPGYNVTGMVNIPVSPNIAVRASG
                     FYHKEGGFIDSPGDDPLFFGPPALGFVILPGSLKEHDYNSDARYGGRLSALFQLNDDL
                     SVRLTALTQNINANGSNLIQINSQTLQPIEGLTYERYNPEFTDSKYRIYDATLNWNLG
                     FANFFSSTSYSDFNQ"
BASE COUNT         1059 a         2176 c         2074 g         1250 t
ORIGIN      
        1 gcccttgtgc agcgctccga ggacggctga ctgcggtacc cgacagtcgt ggaactcgag
       61 gatgctgttg gtgtagccgc gatgcgagac attgcgatag ccgggccgca ccacgaagcc
      121 cggcgtgccc atgtccacca ggaaggcggt gatgagcttg cgcttgccgc gcggtgagtc
      181 ctcctcaccc gaggccgcga acaggatgac gaagtccgaa tgatccgcat ggctgatgaa
      241 atgcttcgtg ccattgatga cgaagctctc gccgtcgcgc acggcagtag tcttcatgcc
      301 gcgaagatcg gatccagctt ccggctccgt catcgccaga cactcggtgc gctcgccgcc
      361 caccgcggga agcagatagc gctcgcgctg ggcaccctcg caggcgagga ggatgttgga
      421 tgggcgcgcc acgcacgagt aatgcagcgc atagctggtc ttgccgagct ctttctcgta
      481 gaggatccag ctcacgccat cgagccctgc cccgcccacc tcgaccggca tgttcgcggc
      541 aaacagtccc gcatcgatgg ctttgcggcg cagctcctgg cgcacggctg ccggcagctc
      601 gcccgtgagc tcgatctgct gctcgtgcgg atacagctcc ttgcgcacga actcgcgcgt
      661 cgtgcggatg atcagctcct gctcttcggt cagcgcgaag tccatcgggc accccctgcc
      721 gtcctgcggt tcacgccgct gaagcgccgg gcgccggcgc cttgtggctc tttctgtgct
      781 tctcgcccac cggcgtcgcc atcgagtcga gcgcccagcc gtgcaggagc tgcagctgca
      841 gacgcagctg ctcgagctgc ctcgcggcgg gccagtcgct ctgactggcg actgcgcttg
      901 cggtgacgcc gttgatgaag gtcaccaccg accgcagcgt ctgctcgcga acgccggagg
      961 cgagctgggg ccagtgcggc caaccctccg ccagacagcg ctcgaagagt cgcatgtact
     1021 cgcggtgaac ccaggcgttg atgcgcttga ggcgcttgtc cgtggacacc tggccccaga
     1081 acgcggtcca gaaggcacac tcgatgtagc gctgctcatc aatgggcaac gcctcggcca
     1141 gcacggtgag cagatcggca gcggtgccgg cgggacgcgt caagcgctcc tcgatccggc
     1201 gcaggatcag acgcaaggcc gcgatgatga tgtcctgctt ggtatcgaag taatgtgcga
     1261 ccatgcccgt ggtgtaaccc gcttcgcgcg cgatcctcac gatggtcgcc tgatcgaagc
     1321 cgtgctcggc gaccacccgg caggcgacca gggcaatctg atcccgacgg tgctcatgat
     1381 cgacgatctt gggcatggcc ttctctcctg ctccggcgcc gggttcagtt acgtggcatg
     1441 ccgatgtgcc ggcccttctg cgcgggggcc ggcaaactcg catgcgccgc gcgaaccgca
     1501 tcgagccggg cgtgcacgct cgcgtccatc gaggatgcct tgcgggctgc cgcatccacg
     1561 tgcaggtaga tctgctccgc tgtggcgacc acgacctcat cgcttgcccg atgcatacga
     1621 tgaaagagat ggacccgctt ctcatccacg ctgagcagtt gggtgttgac cgacagggtg
     1681 tcgaatacct tcgcttccgc ggtgtgtgtc atatgacttt ccaccgcgta gagtgcgcgg
     1741 ccgctcttgc ggtactgatc gttgacgccg acctgacgaa acagcgcatc cgtggcgtcc
     1801 ccgaagacct gcaggtaccg tgagtcggtc atgtgaccgt tgtagtcgag ccattccggc
     1861 cggacctgga cttcgatgag cttcaacggc gcatcgatgg agccgccagc ttgatcgctg
     1921 atgcgggccc cggtcgcccg cgcattctcc tcatagaggc gctcctcgag tgcccggagc
     1981 gtcgcccccg caccgacatc gtgtcgacgc agaacctgct ggatgtccac gaggcagtca
     2041 tcgcgcagcc tctcgagctc gcgaatcgag cgtccccggg cctgcgcctg cgtgccttcg
     2101 accatgcgat cgatgagctg ctcggtcagc tcgggtgctt cgagtcgggt ccagggccat
     2161 ttcagacacg gcccgaactg gcgcagcatg tgccgcatgc ctggctcgcc cccggcgagg
     2221 tgatagatga gattggtgcc catcgctgcc cagcgcagcc cgggcccata gatgatcgat
     2281 tcatcgagct cgccggtagt ggcggtacca tcgttgacca ggtgcaggat ttcccgccac
     2341 agcgcttcct gcagacggtc ggtcagatgc ccggggacct cacgccgcac cttgagcgga
     2401 tgcatgccga tatgcgcata gaacctgctg gccgcatcga ttgcatcggc ggcggtgcgc
     2461 tccccgccca ccagctccac cagcggcagc agatacacgg ggttgaaggg atgtgccacg
     2521 agaaaccgct cgggggcgcg catggcacgc tgcaggtcgg tcggcatcag gcccgaggtg
     2581 ctcgaggcga tgacgacatc gggcggggcc tcctgactga cctcccccag tacgcgctgc
     2641 ttcaattcaa gctgctcagg aatgttctcc tggatgaaat ccacctcgcg cgccatcgcg
     2701 cacagatcgg tggtgaaggt cagctttccc ttcggcggca gcggtgcgag cgtcagacgg
     2761 gccaacgcag gttcggcgtt gcgcaccgcc ccgcgtatcc aatcctccat ttccggcttg
     2821 acgtccgcgg ccacgacgtc gattccgaag tgcagcgccc gcgccgccca gccgccgccg
     2881 atcaccccgg tcccgagcaa tccgagcttc ttcacctgcc tcatgggtcg tccgccagcg
     2941 attcactttt ataatgtctt catcataata taataatcgg accacccggg gcgagaccct
     3001 cggcgcggta aaggggggtg gatgcgcggc ggcgctttgc tcgagacgcg ctcgatcgac
     3061 tacgtgccac tcgccgagcg gcacggcaag gtccgcgatc tgtggccggt gtggttctcg
     3121 ggcgatgccc atctggccac gctggccgtc ggagtcattg gtatcgcgct cgggggtaac
     3181 ctgctgtgga cctccgttgc ggtgcttgct gggtgcgcgc ttggcacgtt cttcatggcc
     3241 ttccattcga cccaggggcc gcagctcggg ctgccgcaga tgatccagtc acgcccgcag
     3301 ttcgggtacg tgggtgcgct gctggtctgg ggcgtagcac tcattgccta catcggctac
     3361 aacgctttca atcaggtgct cgccgcccaa acagtgcatc agctgtggaa gccgatccag
     3421 ccggcctcgc ccaccgtgct gatcgtgttc gccctggtcg cgctcgttcc cgcggcgatc
     3481 ggatacgaca tcatccatct ggcccagcgg gtgtttgcct acctgatgat cgcgatcctg
     3541 ggtgtgttct cgatcgctgc cctgttcgtg ctgcggctcc cgcccgggca gtgggatccg
     3601 gcggcctttc atggcggccc gttcattgcc gaattctttg ccgcggcctc ctatcaactc
     3661 tcctggtcga tctacgtctc cgactactcg cgctacctgc cgcgcagcgt gggcgtccgc
     3721 gagtcgttct ggtggactta cctcggagcc ttcatcggcg gaagctggat gatgttgctc
     3781 ggcacgctgg ccgcagcggc cgccccggga ctcgaggtcg ccgcctcact gcaggtcacc
     3841 ggtaatgcga tcctgcccgg cctgggcgag gtgctgctgg caggtgcgct gctgggactg
     3901 gtcacgatct cgtcgctgaa cttctacggc gcctccctga cgttgctcag cgtcgccgac
     3961 acgttcaaac ccctgcgcag caccgccctg aagcgattcg cgagcctgct cgccaccggc
     4021 attgctggca ccgccatcgc cttcggtgcc tcgggcaact tcctgaccgc cttcggcgac
     4081 ctgctctcgg tgctgctcta cctcttcacg ccctggaccg ccatcaatct ggtggacttc
     4141 tacctcgtgc gccgcggcca ttactcgatc cgagagatat tcaatccaca cggcatgtac
     4201 ggacgttgga actggcgagg tctgctcgcg tatgcaatcg gtttcgtggc catgatcccg
     4261 ttcttcagca ccgagctgta tcggggcccg atcgcccgtg ctctcggggg agcggatatc
     4321 gcgatgctga ttggcctgcc cgtggcggca gtcgtgtatc tgtttgcctg ccgctccctc
     4381 gatgctgccc gtgattggca gctggcgagc gcagcggatg ttggcctgga tccgggtccc
     4441 gagcggctct gagacacgag gacactcgca gcgcccgcgt ggcgcagcac cggggattcg
     4501 aggggcttca gcgtcggcgc gccgggcggc ggcggtgctg caatcggggt gaggcgcgcg
     4561 gtgtgacctc gctcgcctga ccgcctgtca cttcgcgcag atcccgctcg gaggccgagc
     4621 agaccgcgag cagtcggcag ggaccctcgc ctctcacgac ataggcgtgt cccatcgtac
     4681 tgtcgatata ggctgactct ccagcgctca gcacgaccgg ggcatagagc tccgtgtgca
     4741 gctcgacctg gccttccagg acgtagatga attcctcgcc ggtatgtcgg accagctcgc
     4801 caaattcctc gagcgagcgg gcgtgcagct cggtgtggat cgggatgaac ttcttgcgcg
     4861 tgacatcggt gttgaggtag cggtagtcgt agttgcgggt cgcgaccagc tcgccctcgg
     4921 tgcgacggtt gacactgcgg cgcccggtga tggccgccgg acctcctacg ccgttcacgg
     4981 gtgcaaacag ctgcgcgatg tcgatctcga cgccttcgct cagccggatg agcttgtcat
     5041 aagtgagcga caggcggtcg ttctccacct tcgagagcgt gggaatggag aatccggtcc
     5101 gccgactgac ctccgcaagc gtccagccgc gctgctttct cagggcgcgc agcgtctgcc
     5161 cgagccggga tcgtctggcg gtgtctttca tggcgaacgg tgatggatcg gctgacgcaa
     5221 ccatcataga ggagggttgc cagggccgca cctgcacgct ctggcgcgcg ccggggcgtg
     5281 cagtgttcct gaaagatctt tgctacctta tatactaatc taccgttaca gaaacaagtt
     5341 tccaaaacgg caaatccggg gtagagtctg cgcggctgag acagctggcg cgcgcagccc
     5401 ggtaacccag aaagccggcg cgcggggcac caaggcccgc gcttcgcatg cgcgagccgc
     5461 ggcgctgccg gccgtactcg catcccgcaa tgttggtatg gggagctcac gatggaagtc
     5521 ctggcacgag ccctgaaccg ctggatcctt cctgccgcca cgagcatccc gatctggctg
     5581 agcagcgccc cggtgctcgc acagcaggcg tccgcgagcg atcagccggc cacgctggag
     5641 gaagtgatcg tgacggccca gaagcgttcc gagccgctcg ccaacgtccc ccaatccatc
     5701 accgtcgtat cctcggacat cctgcagcgg cagcaggcgt tcgacctgca ggactatctg
     5761 gcactcatcc cgggtctgag cctcaccggg gacactccgg ggcagtcgcg catcaccctg
     5821 cgcggtatca acagcgacgg tgtggcctcg accgtggcgg tgtacgtgga cgacgttccg
     5881 tttggctcca gcagcggtct ggcgaacggc gccatcctgg ccggcgattt cgacacgagc
     5941 gatgtcgcgc ggctggaggt gctgcgcggg ccccagggaa cgttgtatgg cgcgagttcc
     6001 ctcggcggcg tgctcaagtt cgtcaccaac gcgcccaaga ccgacgcgtt cgatgcgaat
     6061 ctcaacgtcg gcgctgagga cctcaacggc ggcggtcccg gctacaacgt caccggcatg
     6121 gtgaacatac ctgtgagccc caatatcgcg gtgcgcgcga gtggcttcta tcacaaggaa
     6181 ggggggttca tcgactcacc cggcgatgat ccgctgttct tcggaccgcc ggccctgggg
     6241 ttcgtgattt tgccgggctc cttgaaggag cacgattaca actctgacgc ccgctatggc
     6301 ggtcggctgt cggcgttgtt ccagctgaac gatgacctga gcgtgcggct gaccgcgctc
     6361 acgcagaaca tcaatgcgaa cggctcgaac ctgatccaga tcaactcgca gacgctgcaa
     6421 ccgatcgaag ggctcaccta cgagcggtac aatcccgagt tcaccgattc caagtatcgc
     6481 atctacgatg cgacgctcaa ctggaatctc ggcttcgcca atttcttttc ctcgaccagc
     6541 tattccgatt tcaaccaga
//
LOCUS       DASRHN010000314        13644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1094806, whole genome shotgun sequence.
ACCESSION   DASRHN010000314 DASRHN010000000
VERSION     DASRHN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13644
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094806"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VFB37_12370"
     CDS             complement(<1..1462)
                     /locus_tag="VFB37_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO23288.1"
                     /translation="MYRAPIRELRFVLEELLQAGELARYAAFADYSNELGESILTEAA
                     RFGESVLDPLNRPGDEQGARWTPDGVVTANGFREAYRQFVEGGWPQLGTEPEYGGQAV
                     PLVLVTAVQEIWASANLAFKLCPMLTHGAVHALTLCGSEELKRRFLPHMVRGEWTGTM
                     NLTEPHAGSDLAQIRTRAVPEGDHYRLFGQKIFITWGDHDCTDNIIHMVLGRIEGAPS
                     GVRGISLFVVPKRLLKDDGTPGEPNEVRCVSIEHKLGIHASPTCVLAYGDRDGAIGYL
                     IGEPNRGLEYMFVMMNAARLSVGLEGYAVGERAYQRALEWARTRIQGKPPGAGTHSPA
                     TIVHHPDVKRMLLTMKASTEAARALALHTALLLDQAKHHPDSGQRTRAQTRADLLIPI
                     VKGWCTEMGNQVVATGVQVHGGMGFIEETGAAQYVRDARITTIYEGTTGIQSNDLIGR
                     KIGRDRGAAMAALLQDMGRELDGLDSRDSAAAAMRKT"
     gene            complement(1519..2430)
                     /locus_tag="VFB37_12375"
     CDS             complement(1519..2430)
                     /locus_tag="VFB37_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO23289.1"
                     /translation="MWTPAQLAVLAKVVDLNGFSAAARALGVPKAAVSRAIADIEKSL
                     GVRLLERTTRRVGLTHAGRLLYPHARRVADESDAARLAIARLNAPPSGPLHVVADPTY
                     GRVLLTPLVPRFLESFPDVPLEVSMQAPAEAQEWDVAIRTKAAGESETMNFRLLGAPP
                     ALLCATPGYLQQRGAPARPEDLRLHDLLTPEPADLPEFRLRLQKNSQRAEVALAPKLA
                     INDPAVLHAATAAGLGIGLLPEFLCRQGLATGRLRPVLEGWTVPPAAALYALYPVALE
                     ADLRVQRFVDFLAANIVPALAAAPGGP"
     gene            2554..3060
                     /locus_tag="VFB37_12380"
     CDS             2554..3060
                     /locus_tag="VFB37_12380"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZO23290.1"
                     /translation="MNVSVYAVSIEVFTSALTNLSGLLEKGAAHATERKFDPAVLVSA
                     RLAPDMLPLSRQIQIACDLAKSSAARLAGQEPPRFEDNEKTFEELRARIAKTLDYLKS
                     IPARALEGAAERDIRVPAGERTLEFKGLAFVQRWAIPNLFFHVTTAYNILRHNGVNLG
                     KRDFLGPM"
     gene            3237..4742
                     /locus_tag="VFB37_12385"
     CDS             3237..4742
                     /locus_tag="VFB37_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO23291.1"
                     /translation="MGKPLLPSNAPTVRPTSDTQAVPIVHAAAATPNEPPTAESLGWQ
                     LRAALPPLRLHSVSLYDDHANVLWLSEGALGPDEHGLVTEAIETLRRETAQSCHENRL
                     EDGRIAIFLAVRAPQGALVGLCMILADIKSVGEGVIERIITPQVRTVLQKIAVLLRPA
                     GRTQTNLALKIVEISAPEALAPAPQVGSTAPGHATEATQVRAALPIPPPPIAPTKAPA
                     TAPTRREAKPSEAGTSGSLPKLAPTSGGTTSTKALQTGATTTSRTLELPEGLTLSLDV
                     QPFTKLRPGGRTRRYEVTARATHRDMQCVPAGLDNFVLQRLLASLAANRASWNAEQAS
                     FTLNLSIATLEDERFPQFIASNLKRHGIAAESIGFEIAEPLCLQKRAQVERFISLCDR
                     IGCFVVIDDFSLDSSAVSLLRSKALRLVKIDPRLTSVALKDKLAQALVVAIAQAVKVL
                     GIHCAAKRVDSQASLQWLTAIGCDFAQGAVLGAVQPLESLTGPAAQSTSPG"
     gene            5148..6266
                     /locus_tag="VFB37_12390"
     CDS             5148..6266
                     /locus_tag="VFB37_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZO23292.1"
                     /translation="MLTLEKLRSNRNVLFWSLHAAGWAAYAVTQYFGALLYEKPASYG
                     QVILVAAGAGFILSMPMRFVFRRLWGRTPRVLFLGVLGTCYVTALALRTVINLAYVQF
                     VDPDWQFKTLFELFGGAVSTTYLLLCWSALYFGIKYYEMQQRQQAATLKAAALAQEAQ
                     LKMLRYQLNPHFLFNTLNAISTLILDNQNRTANNAVTRLSEFLRYTLDQDPVKKVTLR
                     QEIEALDLYLGTERLRFGERLRLEYAVDGPALDALVPSLLLQPLLENSLKYAVSSREQ
                     GGSIRIEGRARGAMLEVSVIDDGPGLRDGAPTSERRGVGLRNTRERLAVLYGENHRFA
                     VLNSHPGLRIDMALPLELAMEPPVQSQPRSAVSQVAGA"
     gene            6263..7084
                     /locus_tag="VFB37_12395"
     CDS             6263..7084
                     /locus_tag="VFB37_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZO23293.1"
                     /translation="MTISTIIVDDEALSRRGIEIRLLTTPDFQVVAQCSNGREALAAV
                     TQHKPDVLFLDIQMPGMSGFDVLAHLPQESLPVVVFITAYDQYAIRAFEAHAVDYLLK
                     PVDDGRFIAALERIRVQVQSRAAASQRDRLVQIIAEMSGSGELALEELLARGPRAAEP
                     RRPGILPIRQGRETVRVAIDSIQWIDAAGDYMCIHAEGNTYILRGTMKELEEVLDPKL
                     FQRVHRSTIVNLSLVKSLRAHMNGEYFLTLEGGHELKLSRTYRDKVEYFLTGTQS"
     gene            complement(7106..8647)
                     /locus_tag="VFB37_12400"
     CDS             complement(7106..8647)
                     /locus_tag="VFB37_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23294.1"
                     /translation="MNRVPRSLSPVLLSVTASVLCAATSSAGATPDDTAITIYSSEQP
                     GAIAPELYRPVPGSSSARMNVPGYALVRQERDVKIDSGRSTLKFADVAALIDPTTVTF
                     TSLSEPRTRVLEQSYQFDLVSTDKLLSKYIDRPIVVEREAGGHAETINGTLLSSVDGL
                     VLRTSDGAIQALRTYSSVRFPELPGGLDTRPTLVWDISSPRAGDQRSRVTYQTGGVTW
                     WTDYNLIFAEAQDANTGTLDLSAWVSIINQSGATYHDARLKLIAGDVHRVAPEMRGSA
                     ARYAMQAAVADAAAAPQFTEQPFFEFHLYALQRRTTLPDNSTKQIELFDEARQIPARK
                     VLMYAGAAPSYAYAGPLTERNYGVQSNKKVDVYLSFRNEKARGLGIPLPAGRIRVSKL
                     DPSDGSLEFIGEDRIDHTPRDEEVRVKLGSAFDVVGERRQTDFAVDTHARSMEEQIEI
                     RLRNHKEQPVQVLVKENLYRWSNWKILTHSQDFRKEDAHTIIFPVEVPKDGETVVSYR
                     VRYTW"
     gene            8936..10333
                     /locus_tag="VFB37_12405"
     CDS             8936..10333
                     /locus_tag="VFB37_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23295.1"
                     /translation="MTDLPVEEPLGVETPQMREPAAEVALEIPAPTRAVDPSPTLETF
                     EAEELLAHGVLLDGAAALAAASPAPAQTISAAPVVTAPVLAPAPTAPNVPAAASSAAM
                     PAALPGANPPTPAALGAAPVAIPPVAPPVLSPRPTSMPVGPIPAPARPVTGARAEPPS
                     MPIARPPPVIPVRVSAMAAPSASATPPALQAAVLSHPVAATAAMPAPALPSSMAGGVT
                     TASLASSAGSSATTGAMDRDFIARNQIVERYLSGRLPLKGATDFERFCRDNPQLLDEL
                     GLAERVNAGLRLLEASGKPDPWQEAPKSAWTKPQFVLGLAAVVLALSVALAVVGSSSA
                     TKSQKIVRLQRQVAEQPLDPATSTRTIRLVPSHDGASNTPAVVIGGGPAQLVDLKIDE
                     SRSAYKTFQITIDRIDQGRVAILRNLVKDSNGHLRIALNSSALGPGNYQLTIEGLSWR
                     GDPEPDAWITIGIQR"
     gene            10343..11581
                     /locus_tag="VFB37_12410"
     CDS             10343..11581
                     /locus_tag="VFB37_12410"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZO23296.1"
                     /translation="MSSAPKTGPPQVDVFVARQPIYDASMAVVAFELLYRPSPTAAAA
                     RIRDARQATLEVISSAALEIGLDRLSGGLPVHINFPRELLVDVPDLPLPPAQVIIEVL
                     EDVRAEPGVIEGILKLRGRGHRIALDDYSPEVSDERLLEFADIVKLEISHPPAGELAR
                     RVQALKAGGLKLIAENVESPEQFEQCIALGFDGFQGYFLQHPQTFRAKRVPSSRLSTL
                     RLVASLQNEEYSIDEVEALLSQNVSMSYHVLRCINSSYYNLPRKVGSIRQAIVILGLE
                     HLRQLCTLLCLQGFDDRPPSLFVNAMTRARMCEQLGRLGGAKDAGPFFMTGLFSLLNA
                     MVGLPIQKIVEELPLTPAITRALVTGEGDLGAALQCTRAYERAAWDQVSYKDLPAPLI
                     RAAYVDALFWAEQARKLIDK"
     gene            complement(11584..>13644)
                     /locus_tag="VFB37_12415"
     CDS             complement(11584..>13644)
                     /locus_tag="VFB37_12415"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZO23297.1"
                     /translation="KLLAAIDAHVGSQLSIGAATFRVTHVLISRPDQGGTFAELAPTL
                     LMNEADLSSTQLIQPGSRVSYRALFAGDRDRIDVFKSWLMAHKKRSERVHDITDASPQ
                     IRNAVERAGRFLSLASLVSVLLCAIAVAMSARRYVQRHLDSVALLKTLGATRAFILTV
                     STVQLLALAGLAALLGSGAGFLAQEWLLHTIKDLLAVQLPPPSLLPIGMGFLTAVAVM
                     VGFALPPMLQLVRVPALRVLRRDVGPPPPLMLLAFGPAVVIVIGLVYWVVRDWKLFLG
                     FSGGLAAFLLTLTVAGALLVLLAGRLRSRVGVAWRFGVANLSRRRVDSLVQIVAFGTG
                     MMVLLLLSIVRDDLASDWRRTLPANLPNYFFVNIPPGERDSFVRFLQEHGAKTARMLP
                     MIRGRLTSIRGRSVDELKFGGEEGENFATREQNLTWAAEPGADNRIVAGEWWTAADFG
                     KPLVSLATEFQDSLGIHVGDRLTFDIGGEPFDVQVASIRKVKWDSFQPNFFVVFAPGV
                     LDRAAGTYITSANLKPGDARSLAQLARRFPSVSIFDIDDLLAEVRSVLDKAVLAVQSV
                     FIFTLFAGLTVLLAAVQSSRDERRYESAMLRTLGASRRTVLQGVLAEFATLGLLSGGL
                     AAAGAAIAAYFIESRVLQLHYSFDWRVGLVGLCGGALLVAATGWLATRSVVRQPPLRT
                     LRAD"
BASE COUNT         2459 a         4536 c         4324 g         2325 t
ORIGIN      
        1 ccgtcttgcg catcgcagcc gcggcggaat cccggctgtc gaggccgtcg agctcgcgcc
       61 ccatatcctg cagcagcgcg gccatggctg cgccccgatc gcgaccgatc ttgcgtccga
      121 tgagatcgtt cgactggata ccggtcgtgc cctcgtaaat cgtcgtgatg cgcgcgtcac
      181 gcacatactg agcggcgccg gtttcctcga tgaagcccat gccaccgtgc acctgtacgc
      241 ctgtggccac gacctgattc cccatctcgg tgcaccagcc cttgacgatc gggatgagca
      301 ggtcggctcg ggtttgagcc cgggttcgct gcccggaatc cggatgatgt ttggcctgat
      361 cgagcagcaa ggcggtatgc agcgcgagcg cccgcgcggc ttcggtcgag gccttcatcg
      421 tgagcaacat gcgcttgacg tccgggtggt ggacgatcgt cgccgggctg tgagtccccg
      481 cgcccggcgg cttgccctgg atgcgcgtgc gcgcccactc gagtgcccgc tgatacgccc
      541 gctcacccac ggcatagcct tcaaggccga cggacagccg tgcggcgttc atcatgacaa
      601 acatgtattc gagaccccgg ttgggctccc cgatgaggta gccgatcgcg ccgtcccgat
      661 cgccgtaagc gagcacacaa gtggggctcg catggatgcc gagcttgtgc tcgatcgaca
      721 cgcagcgaac ttcattgggc tcgccgggcg tgccatcgtc cttcagcagc cgcttgggta
      781 cgacgaaaag agagatgccc cgcacgccgg acggcgcccc ctcgatacga cccaacacca
      841 tgtggatgat gttgtccgtg cagtcgtggt ctccccaggt gatgaagatt ttctgcccga
      901 agagccggta gtgatcgcct tcggggaccg cgcgggtgcg gatctgcgcc aggtccgagc
      961 cggcatgcgg ctcggtgagg ttcatcgtgc cggtccattc cccccgcacc atgtgtggca
     1021 ggaagcggcg cttcagttcc tccgagccac acagcgtcag cgcgtgcacg gcgccatgcg
     1081 tgagcatcgg gcaaagcttg aacgcaagat tggccgaggc ccagatctcc tgcacggcgg
     1141 tgacgagcac gagcggtacc gcctgcccgc catattcggg ctccgtgccg agctggggcc
     1201 agccgccctc gacgaactgc cggtatgcct cgcgaaatcc attcgcggtc acgaccccgt
     1261 cgggcgtcca gcgcgcaccc tgctcatcgc cgggccggtt caatggatcg agcacactct
     1321 cgccgaagcg cgccgcctcg gtgagaatcg actcgccgag ctcgttggaa tagtcggcaa
     1381 aggccgcata gcgagccagc tcaccggcct ggagcagctc ttcgagcacg aaacgcagtt
     1441 cccggatcgg agcacgatac atgtcagaga tccttgcagc cgcgcagtcc cggcggagtc
     1501 gatgtcatcc cgcgcacctt acggcccacc aggagccgcc gccagagccg gcacgatgtt
     1561 cgcggcgaga aaatccacga agcgctgcac acgcaggtct gcctcgagcg cgacgggata
     1621 cagcgcgtac agcgcggcag cgggtggcac cgtccatcct tccagcaccg gccgcaaccg
     1681 gccggtcgcc agaccctgcc ggcacaggaa ttccggcagc agcccgatgc ccaggcccgc
     1741 tgcggtcgcg gcgtgcagaa cggctgggtc gttgatggca agcttgggag ccagcgcgac
     1801 ctctgcgcgc tgcgagttct tctgcaggcg caaacgaaac tcgggcagat ccgcaggctc
     1861 gggcgtcaac agatcatgca gccgcaggtc ttcgggacgc gccggagcgc cgcgttgctg
     1921 gaggtagccg ggcgttgcgc acagcagcgc cgggggtgct cccaggaggc ggaaattcat
     1981 ggtttcgctc tcgcccgctg ctttcgtgcg aatggccacg tcccattcct gcgcctcggc
     2041 cggtgcttgc atggaaactt ccagggggac atccggaaag ctctcgagga acctcggtac
     2101 cagaggcgta agcagcacgc gcccataagt tggatcggcc accacatgca gcggcccgct
     2161 cgggggtgcg ttcaatctcg caatggcgag gcgcgcggca tccgattcat ccgcaactcg
     2221 ccgggcatgc ggatacagaa gccggccggc atgcgtgagg ccgacgcggc gcgtcgtacg
     2281 ctccagaaga cgcactccga gagacttctc gatatcggcg atcgcgcgac tgacggccgc
     2341 cttgggaact cccagtgcac gcgcggctgc cgagaagcca ttcaggtcca cgaccttggc
     2401 cagtacggct agctgcgcag gggtccacat caggctcaag gtgcatctcg gtgacggctt
     2461 cgaccggagc ctattgtttc acccaggaag cgtccggtcc aagccgcggg caatgtaaaa
     2521 tgcgcccaca ccccccgggg ccgaggagca aaaatgaacg tgtccgtcta cgccgtttcc
     2581 atcgaggtct tcacgtccgc actgaccaac ctctcggggc tgctggagaa gggcgcggcg
     2641 cacgccacgg agcgcaaatt cgaccctgcc gtcctcgtgt ccgcgcggct cgcccccgac
     2701 atgttgccgc tctccaggca gatccagatc gcctgcgacc tggcgaagag ctcggccgcc
     2761 cgcctcgcgg ggcaggaacc gccacgcttc gaggacaacg agaagacgtt cgaggagctg
     2821 cgcgcacgca tcgcaaaaac cctcgattac ctgaaaagca ttcccgcgag agctctggaa
     2881 ggcgcggcag agcgtgacat ccgcgtgccc gccggcgagc gaacgctgga gttcaagggg
     2941 ctcgccttcg tgcagcgctg ggcaatcccc aacttgttct tccacgtgac cactgcatat
     3001 aacatcctgc gtcacaacgg cgtgaatctc ggcaagcgcg attttctcgg accgatgtag
     3061 gtttcgacag tcagcgggca catcgtcaaa tcgatatgac aaaccgcgcg cgccgcgcgc
     3121 ggaaaaatgt ctgcatccgc cgacaaacgg gaaagcagtc acatcactgc tcgcttcatc
     3181 ctgccaccct agtttccgag cgccggcctc cttgcttgcg cacgctgcac atctggatgg
     3241 ggaagccctt gctgccgtcg aatgctccca ctgtccgtcc aacctccgac acgcaggctg
     3301 tacccatagt ccatgcagcc gcggcgacgc cgaatgagcc gccaaccgcg gaatcgcttg
     3361 gctggcagct gcgtgccgcg ctgccgcccc tgcgtctgca ctcggtttcc ctctacgacg
     3421 atcacgccaa tgtcctgtgg ctgagtgagg gcgcactcgg gcctgacgag catgggctcg
     3481 tgaccgaagc catcgagacg ttacgccggg agaccgcgca gagctgccac gaaaatcgcc
     3541 tcgaggatgg ccggatcgca atcttcctcg ccgtccgtgc cccgcagggc gcactggtcg
     3601 gcctctgcat gatcctcgcg gacatcaaat cagtcggcga aggcgtcatc gagcggatca
     3661 tcactccaca ggtgcgcacg gtgctgcaga agattgccgt gctgctgcgc cccgcgggac
     3721 gtacgcaaac caaccttgct ctgaaaatcg tggaaatctc ggcgccggag gccctggcgc
     3781 ccgcaccgca ggtaggcagc accgcaccgg gccacgccac ggaagcgact caagtgcgag
     3841 ctgcgctgcc gattccgccg ccacccattg cgcccaccaa ggcgccagcc accgcgccca
     3901 ctaggcggga ggccaagccg agcgaagcgg gcacctcggg gagtctgccc aagctggcac
     3961 cgacatccgg aggcaccacc tcgacgaagg cgttgcagac aggagccacg acgacctccc
     4021 ggacgctcga gctgcccgag ggccttactt tgtcactgga tgtgcagccg ttcaccaagc
     4081 tacgcccggg cggccggacg cgacgctatg aggtgaccgc gcgcgcgaca catcgggaca
     4141 tgcagtgcgt accggcgggg ttggacaatt ttgtgctgca gcggctgctt gcgtcgctcg
     4201 ccgcgaatcg ggcgagttgg aatgcagagc aggccagctt caccctgaat ctctcgattg
     4261 caacgctcga ggacgagcgc tttccgcagt tcatcgcctc caacctcaag cgccacggca
     4321 tcgccgcgga aagcatcggc ttcgagattg ccgagccgct ttgcctgcag aagcgggcgc
     4381 aggtcgagcg cttcattagc ttgtgcgacc ggatcggctg cttcgttgtg atcgacgact
     4441 tctcgctcga ctcctcagcc gtgagtctgc tgcgctccaa agcgctgcgc ctggtgaaga
     4501 tcgatccgcg cctcacctcg gtcgcactca aggacaaact cgcgcaggca ctggtcgtgg
     4561 ccattgcaca ggcggtgaaa gtcctcggga ttcattgcgc cgccaagcgc gtcgattcac
     4621 aggcctcgct gcaatggctc accgccatcg gatgcgactt cgcgcaggga gctgtgctcg
     4681 gcgcggtgca gcctctggaa tcgctgacgg gccctgcagc tcaatcaacc tccccaggtt
     4741 gaaagccccg cccgcccgga ttcgtggcgg tggccggcgt ccagatctat acctgagccg
     4801 ctaccagcac agcggccgcg atcagcgccg cagcgctgag ccccagaacg acctttcgca
     4861 tgcttgcacc tcctgcgata ccctttggct cgatcctctt tgcgccgcaa gggctatgcc
     4921 acgcaaagct tgcaaggaac acctgaccgg ctgcccggac tccgggtacg gatcaagggt
     4981 aatggaatca atggcttgtt gagggtcagc ggtgcctacg cggacctgct gcgaggggcg
     5041 ctcgaaacgg gagtgcgaca aggcgagcgg cgcgcgcgtt cgccaggcgc gcgcctgttc
     5101 gcagatgtcc taagcttcgc ctaggaccga ctttcagcgg cgtctggatg ctgactctcg
     5161 aaaaactgcg atcgaaccgg aacgtgcttt tctggagcct ccacgccgcg ggctgggcag
     5221 cctatgcggt gacgcagtat ttcggcgcgc tgctctatga gaagcccgcg agctacggcc
     5281 aggtgatcct ggtggccgcg ggtgccggtt tcatcctctc gatgcccatg cgattcgtct
     5341 tcaggcggct ctggggccgc acgccgcgag tgctgttcct tggcgtgctg ggcacctgtt
     5401 acgtcacggc tttggcgctg cgcaccgtca tcaatctcgc ctacgtgcag ttcgtggatc
     5461 ctgactggca attcaagacc ctgttcgagc tgttcggtgg cgccgtgtcc acgacctatc
     5521 tgctgctgtg ctggagtgcg ctgtacttcg gcatcaagta ttacgagatg cagcagcgcc
     5581 agcaggccgc aacgctcaaa gccgctgccc tggcacagga ggcgcagctg aagatgctgc
     5641 gctatcagct caatccgcac ttcctcttca acacactgaa tgccatcagc acgctgatcc
     5701 tcgacaatca gaaccgcacc gcaaacaacg ccgtcacacg cttgtcagaa ttcctgcgct
     5761 ataccctgga ccaggatccc gtcaagaaag tcaccttgcg ccaggagatc gaggctctgg
     5821 atctgtacct ggggaccgag cggctgcgct tcggcgagcg gctgcggctc gagtacgccg
     5881 tcgacggccc ggcgctcgat gcgctggtgc cgagtctgct gctgcagccg ctgctcgaga
     5941 actcactcaa gtacgccgtc tcctcgcgcg agcagggtgg ctcgatccgc atcgaaggtc
     6001 gtgctcgcgg agcaatgctc gaggtgagtg tgatcgacga tgggccgggt ctgcgcgacg
     6061 gcgctcccac gagcgagcga cgaggggtag gattgcgcaa cacgcgcgaa cggctggccg
     6121 ttctgtacgg cgagaatcac cgcttcgccg tcctcaacag tcatccagga ctgcgcatcg
     6181 acatggcctt accgttggag ctggcaatgg agccgcctgt tcagtcgcag ccgagatcgg
     6241 cggtgtcgca ggtcgccggc gcatgacgat ctcgacaatc atcgtcgacg acgaagcgct
     6301 gtcacgccgc gggatcgaga tacgattgct gaccacgccg gacttccagg tcgtggctca
     6361 gtgctcgaat ggccgcgaag cgctcgctgc cgtgacgcag cacaagcccg atgtactgtt
     6421 tctggacatc cagatgcccg gcatgtccgg attcgacgta ctggcgcatc ttcctcagga
     6481 gtcgctgccc gtcgtagtgt tcataacagc gtacgaccag tacgcgatcc gcgccttcga
     6541 ggcgcatgcg gtcgactacc ttctcaagcc ggtcgacgat ggccgcttca ttgcagccct
     6601 ggagcgtatc cgcgtgcagg tgcagtcgcg ggctgcagcc agtcagcggg accggctggt
     6661 gcagatcatc gccgagatga gcggctcggg cgagctggcg ctcgaggagc tgctcgcgcg
     6721 tggaccgcgc gccgcggagc cgcgcaggcc cggcattctg ccgatccgcc agggccggga
     6781 aaccgtacgt gtggccatcg attcaatcca gtggatcgat gctgccggcg attatatgtg
     6841 cattcacgcc gaaggtaaca cctacatcct gcgcggaacc atgaaggaac tggaggaagt
     6901 gctcgacccg aaactctttc agcgcgtgca ccgctcgacc atcgtgaacc tgagtctggt
     6961 gaagagcctg cgggcgcaca tgaatggaga atacttcctc acactggaag gtggccatga
     7021 gctcaagctg tctcggacct atcgtgacaa ggtggagtac ttcctgacgg gcacgcaaag
     7081 ctgagctggc ccagacccct gccgatcacc aggtgtagcg cacccgatag ctgaccacgg
     7141 tttcgccatc cttcggcacc tcgaccggaa agatgatggt gtgcgcatcc tccttccgga
     7201 agtcctgtga atgggtcaaa atcttccagt tgctccagcg atagagattc tcctttacca
     7261 gcacctgcac cggctgctcc ttgtggttgc gcagccggat ctcgatctgc tcctccatcg
     7321 agcgggcgtg agtgtccacc gcgaagtcgg tctggcggcg ctctcctacg acgtcgaagg
     7381 cggatccaag cttcacgcgc acttcctcgt cccgtggtgt gtgatcgatg cgatcctcgc
     7441 cgatgaactc gagactgccg tcgctcggat ccagcttgga tacccggatc cgacctgcgg
     7501 ggagtggaat gcccagaccg cgggccttct cattgcggaa cgagagatag acgtccacct
     7561 tcttgttgga ctggacgcca tagttgcgct cggtgagcgg ccccgcatag gcatacgagg
     7621 gcgccgcccc ggcatacatc agcaccttcc gggctgggat ctgacgcgcc tcatcgaaca
     7681 gctcgatctg cttggtcgaa ttgtctggca gggtcgtccg ccgctgcagc gcgtacaggt
     7741 gaaattcgaa aaagggttgc tcagtgaact gcggagccgc agccgcatcg gcaaccgccg
     7801 cttgcatcgc ataccgcgcg gcgctccccc gcatctccgg tgctacccga tgtacgtccc
     7861 cggcgatcag cttcagacgg gcatcatgat aggtcgcgcc cgactggttg atgatgctga
     7921 cccaggcgga cagatcgaga gtgccggtgt tggcgtcctg ggcttcggca aagatcaggt
     7981 tgtagtccgt ccaccaggtg acgccaccgg tctggtaggt cacgcggctg cgttgatcac
     8041 cggcgcgcgg cgaagagatg tcccagacga gtgtcgggcg agtgtcgagc ccgccgggca
     8101 actcgggaaa gcggaccgac gagtaggtgc gcagcgcctg gatcgcgccg tcactggtgc
     8161 gcagcacgag tccatccacc gaggacagga gcgtgccatt gatcgtttct gcgtgtccgc
     8221 cggcctcgcg ctcgaccacg atcgggcgat cgatgtattt cgagagcagc ttgtccgtgc
     8281 tgaccagatc aaactggtag ctctgctcga gaacccgcgt gcgaggctct gagagcgagg
     8341 tgaacgtgac cgtggttgga tcgatcaacg cggccacatc ggcaaacttg agtgtcgagc
     8401 ggccgctatc gatcttgaca tcacgctcct gccggacgag cgcgtaacct gggacgttca
     8461 tcctggccga ggaggatccg ggtaccgggc ggtaaagctc gggcgcaatc gcaccgggtt
     8521 gctcggagct gtagatggtg attgcggtat catccggggt ggcgcccgca cttgaggtcg
     8581 ccgcgcacag cacggacgcg gtgactgaca ggaggactgg cgatagtgag cgcggtacgc
     8641 gattcatgac tcgtggccct cgggtgctac ggatgacacc tcttaacgtg gccaaaggct
     8701 caacgggtca aggtagagaa tactcggcgc tcggtcacta aattgaggat ccggggaggg
     8761 actcccggac ggctgcaaat gtgatgtgcg tcacgaaccg gggggcggtc cgccgatctg
     8821 tgagccatcg cgcaggtgcc atcgagccgc cattgcagga tagctcgagg atactgatct
     8881 taatcgctcc ggctcgaaac ctatggaagc agcgtcgcgc cccaactttg aggccttgac
     8941 cgatttgccc gtcgaggagc cgctcggggt ggaaacgccc cagatgcgcg agcccgctgc
     9001 cgaggtcgcc ttggagatcc cggcgccgac gcgggccgtg gatccgtcgc ccacgctgga
     9061 gaccttcgag gcggaagagc tgctggcgca cggcgtcctc ctagacggtg ctgccgcgct
     9121 cgccgcggct tcccctgccc cggctcagac gatctccgct gcgcccgttg tcacggctcc
     9181 ggtgctcgcg ccggcgccca ctgcacctaa cgtgcccgct gcggcatcca gcgctgccat
     9241 gccagccgcc ctgcccgggg ctaacccgcc gacgcccgca gcgctcggtg ccgcgccggt
     9301 cgcaattcct cccgtggcgc cgcctgtact cagcccgcgc cccacgagca tgcccgtggg
     9361 gcccatcccc gccccagcgc gacccgttac gggcgcgcgc gccgaaccgc cttcgatgcc
     9421 gattgcgcgc ccgcctcctg tcattccggt gagagtgtcg gcgatggcgg ccccctcggc
     9481 ctccgcgacg ccacccgcac tgcaggccgc ggtgttatcg catcctgtgg ctgcgactgc
     9541 ggcgatgcct gcgccggccc tgccgagctc tatggccggt ggcgtgacga cggcgagtct
     9601 agccagctct gcgggatcct ccgctacgac gggggcgatg gatcgggact tcatcgccag
     9661 aaatcagatc gtcgagcgct atctgtccgg acgccttccc ctgaaggggg cgacggactt
     9721 cgagcgcttc tgtcgcgaca atccgcagct gctggacgag ttgggtctcg ccgagcgcgt
     9781 gaacgcgggc ctgcggctgc tcgaagcgag cggcaagccg gacccctggc aggaggctcc
     9841 gaagagcgcc tggaccaagc cgcaattcgt gctcggcctc gccgcagtcg tcctggccct
     9901 gagtgtcgcg ctggccgtgg tcggaagcag cagcgccacc aaatcgcaga agatcgtcag
     9961 gttgcaaagg caggtggccg aacagccgct cgatccggca acgagcacgc gcaccatccg
    10021 tctggtgccc agccacgatg gggcttcgaa cacaccggcc gtcgtcatcg gcggtggccc
    10081 tgcgcagctc gtcgatctga agatcgacga gtcgcgctcc gcgtacaaga cctttcagat
    10141 cacgatcgat cgcatcgacc agggccgcgt cgccatcctg cgcaacctgg tcaaggactc
    10201 caacggtcat ctgcggatcg cactgaacag ctccgcgctt ggccccggaa actatcagct
    10261 cacgatcgaa gggctctcct ggcgcggtga tcccgagcct gatgcctgga tcaccattgg
    10321 catccagcgc tgaagtccca tggtgagcag cgctccgaag accgggccgc cgcaagtcga
    10381 tgtcttcgtt gcgcgtcaac ccatttatga tgcgtcgatg gccgtcgtgg cattcgagct
    10441 gctgtatcgg ccttctccca ctgctgccgc cgcacggatc cgcgatgcac gccaggccac
    10501 cctcgaagtc atctcgagcg ctgccctgga gatcggtctc gaccgcctct cgggcggcct
    10561 gccggttcac atcaacttcc cgcgcgagct gctggtggac gtgccggacc tgccgctgcc
    10621 gccggctcag gtcatcatcg aggtcctgga ggatgtgcgc gccgagcccg gggtgatcga
    10681 gggcatcctg aaattgcgcg gccgcggcca tcgcatcgct ctcgatgact actcgcccga
    10741 agtgagcgat gagcggctgc tcgagttcgc cgatatcgtg aaactcgaga tttcgcaccc
    10801 ccccgccggc gagcttgcgc gccgggtgca agcactgaag gcaggcggcc tcaaactgat
    10861 cgccgagaac gtcgagtcgc cggagcagtt cgagcagtgc atcgcgctgg gattcgatgg
    10921 ctttcagggt tattttctac agcatccgca gacctttcgg gccaagcgcg taccctccag
    10981 ccgcctgagc acgctgcgcc tggtcgcctc gttgcagaac gaggagtact cgatcgacga
    11041 ggtcgaagcg cttctcagcc agaacgtgtc catgtcgtac cacgtcctgc gctgcatcaa
    11101 ctccagctac tacaacctgc cgcgcaaggt cggctcgatc cgccaggcca tcgtcatcct
    11161 cggtctcgag catctgcgcc agctgtgcac actgctgtgt ctgcagggct tcgatgaccg
    11221 gcctccgagc ctgttcgtca acgcgatgac aagggcgcgg atgtgcgagc agctcggccg
    11281 gctgggcggc gccaaggatg ccgggccgtt cttcatgacc gggctgttct cgctgctgaa
    11341 cgccatggtc ggcctgccga tccagaagat cgtcgaggag ttgccgctga ctccggcgat
    11401 caccagggcc ctcgtgacgg gcgagggcga tctcggcgcg gccttgcagt gcacacgcgc
    11461 ctacgagcgt gcagcctggg atcaggtttc ctacaaggat ctgccggcgc cgctcatccg
    11521 cgcggcctat gtggatgcgc tcttctgggc agagcaggcg cgcaagctga tcgacaagta
    11581 ggctcagtcc gcacgcaatg tcctcagcgg gggctgacga accaccgagc gcgtagcaag
    11641 ccagcccgtg gccgccacga gcagcgctcc gccgcacagc cctacgagac caacacgcca
    11701 gtcgaagctg tagtgcagtt gcagtacgcg cgactcgatg aaataggccg caatggcggc
    11761 ccccgccgcc gcgagccccc ctgagaggag cccgagcgtg gcgaactccg cgagcacacc
    11821 ctgcaatacc gtcctgcggc tggccccaag cgtacgcagc atggcgcttt catagcgtcg
    11881 ctcatcgcgg ctcgactgca ctgcagccag cagcaccgtc aagcctgcga acagcgtgaa
    11941 gatgaagacg ctctggaccg cgagcaccgc cttgtccagc acagagcgga cttccgccag
    12001 cagatcgtcg atgtcgaaga tggagacgct ggggaaacgc cgcgcgagct gagccagcga
    12061 gcgcgcatca ccgggcttga gattggcgct cgtgatgtag gtgcctgcgg cgcgatcgag
    12121 gaccccgggg gcaaagacga cgaagaagtt gggctggaag ctgtcccact tcaccttgcg
    12181 gatgctcgcc acctgcacat cgaacggttc gccgccgatg tcgaaggtga gacggtcgcc
    12241 cacgtggatg ccgagcgaat cctggaactc ggttgccaga gacacgagcg gcttgccgaa
    12301 atcggccgcc gtccaccact ccccggccac gatacgattg tcggcacccg gctcggcggc
    12361 ccaggtcaga ttctgctcgc gcgtcgcgaa attctcgcct tcctccccgc cgaacttcag
    12421 ctcatccacg gagcggccgc gaatactggt cagccgcccg cgaatcatcg gcagcatgcg
    12481 cgcggtcttc gcaccgtgct cctgcaggaa gcgcacgaac gaatcgcgct cgcccggggg
    12541 aatgttgacg aagaaatagt tgggcaggtt ggccggcaac gtgcggcgcc agtcgcttgc
    12601 caggtcatct cgcacgatgc tcagcagcag cagcaccatc atgccggtac cgaaggccac
    12661 gatctggacc agactgtcga cgcgacgccg gctgaggttc gcgacaccga agcgccaggc
    12721 aacgcccacc cggctgcgca gtcttcccgc gaggagaacg agcagggcgc cggccaccgt
    12781 gagggtcaac aggaacgccg cgaggccgcc gctgaatccc aggaaaagtt tccaatcgcg
    12841 caccacccag tacacgagac cgatcacgat cacgacagcc ggcccgaacg ccagcagcat
    12901 caaggggggc ggcggtccca catcgcgccg cagaacccgc agcgcaggaa cccgcaccag
    12961 ctgcagcatg gggggcaatg cgaagcccac catgaccgcc accgcggtca gaaagcccat
    13021 cccgatgggc aagagactcg gcggcggcaa ttgcacggcg agcaggtcct tgatggtgtg
    13081 cagaagccat tcttgcgcga gaaagcccgc cccgctgccc agcagggctg ccaggcctgc
    13141 caaggctaga agttgcacgg tgctgaccgt caggatgaac gcgcgcgtcg ccccgagagt
    13201 cttcagcagt gcgacactgt ccaggtggcg ttgaacgtag cggcgcgccg acatcgcaac
    13261 ggcgatcgcg caaagcaaga cgctgacaag gctggcgagg ctcagaaagc gcccggcacg
    13321 ttcgactgca ttccggatct ggggactggc atcggtgatg tcgtgcacgc gctcgctgcg
    13381 cttcttgtgc gccatgagcc aggacttgaa cacgtcgatg cggtcgcggt ccccggcgaa
    13441 caacgcacgg tagctgacac ggctgccggg ctgaatcagc tgagtcgatg acagatcggc
    13501 ctcattcatc aacagggtcg gggcgagctc ggcgaacgtg ccgccctggt ctggacgcga
    13561 gatgagcacg tgtgtgactc gaaaggttgc ggcaccgatc gagagttgcg agccgacgtg
    13621 cgcatcgatc gccgccagca gctt
//
LOCUS       DASRHN010000315         8009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1266229, whole genome shotgun sequence.
ACCESSION   DASRHN010000315 DASRHN010000000
VERSION     DASRHN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266229"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFB37_12420"
     CDS             complement(<1..398)
                     /locus_tag="VFB37_12420"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HZO23298.1"
                     /translation="MAAPKSKAERPLIIVKRKGRQGHGHHGGAWKVAYADFVTAMMAF
                     FLVMWLVTAVSKDQRAAIFDYFKNPSMEPGKTSKPAPGQAGPGGASTSPIDLHGGLDA
                     VRTRTAPVMDFGATAAPTPMRREENSDGAGP"
     gene            complement(411..1325)
                     /gene="motA"
                     /locus_tag="VFB37_12425"
     CDS             complement(411..1325)
                     /gene="motA"
                     /locus_tag="VFB37_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HZO23299.1"
                     /translation="MRRIAPSATRVTASHPVIQIVGSIVVVGCVIAGFVLEGGKLLAL
                     WHPTEVLIIVGSAFGAFLTSNPPKVTKAAFMGALGLVKGPRYKRDDYVELLKCLYDVL
                     VKIRKEGLMAIEGDLEAEDGGALFRNYPKIVADHHLMDFIRDCLRLMIGGNLDPHELE
                     SLLEQELESHHQEAHEPAQAVQKVADALPGFGIVAAVLGIVNTMAALDGADTASIGHK
                     VGAALVGTFLGILVSYGLVGPIAAAMEHAANEEGKAFSVVKMVLVAAVRGYVPAVAIE
                     FARKLLSQDIRPKFSDLETQLKAKAKAA"
     gene            1726..2379
                     /locus_tag="VFB37_12430"
     CDS             1726..2379
                     /locus_tag="VFB37_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO23300.1"
                     /translation="MSAHVLTPADRDEAAAKPIELTRPVREPEIIDAAARVITRRSDH
                     RILWSTIAHEAGHCAAPIETCFDGLLTLIDACYSRTAQGLSHSLLQAETAPGTALDQV
                     AAFLVSALEIRRSRGVFLSFRRCADLPGSLQRRLHEYDAMTRTRLKRLLQRGHRDGSL
                     AHRQPDAAVELILACLQVPTVVTDGPEQRMWDGELVELLLAGLSEPHPPEDGTRLLG"
     gene            2514..3710
                     /locus_tag="VFB37_12435"
     CDS             2514..3710
                     /locus_tag="VFB37_12435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZO23301.1"
                     /translation="MMFERLHRLQTDPILGLMAAFRADTSPRKVDLGVGVYRDDHGET
                     PVLGCVRRAEEAVLARQSTKSYVSSVGNVGFNQAMERLVFGESHAALSSDRVRTIQAP
                     GGCGALRLGAELIRNAWPDTVIHVSTPTWANHVPLLSGAGLKLERYPYYDPASGGVRF
                     ESMLAALETLPAHAVVLLHASCHNPTGADLSEAQWRELLSVIRRRTLLPFIDMAYQGL
                     GSGTAEDAFAIRLFCEELPEVAVAVSCSKNFGLYRERTGCLHVLSDSAKSGDAALSHL
                     VRIARSLYSMPPDHGAAIVHEILGNPALWQEWTGEVGGMRARLTGLRQELVKHLGRAC
                     PQRDFHFIAAQRGMFSFLGVDTAQVHALRERHHIYMTDDSRINIGGLRADNLEYFAQA
                     VAQVLG"
     gene            3747..5828
                     /locus_tag="VFB37_12440"
     CDS             3747..5828
                     /locus_tag="VFB37_12440"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23302.1"
                     /translation="MKRFVLVVISAVVSVLSIVNAQEAGKETAPAVEAPGTTAAQAAT
                     PLNPERATAGVDRSGTSRGRVPVPQNVPYPGTLTLKVDLTDLDRRVFTVRESIPVKPG
                     PLTLLYPEWLPGNHAPRGPIDALAGLTLMAADHRIEWTRDAVNVYAFHLDVPAGANRL
                     DVQFQFASPFLHEQGRTVVTPDIVGLQWNTVVLYPAGYYSSRISMLPSVVLPAGWAFA
                     SALDVDSRGGNQVSFRPTTLEMLVDSPLFAGHYFRSVDLDPGAAVPVHLNMVADSPES
                     LEIKGPELVAHRKLVQEAYALFGGPHYDHYEFLLALSDHFGEIGLEHHRSSENRRPPG
                     YFTDWDKSGALRDLLPHEFSHSWNGKFRRPEGLATPDFNTPMRDSLLWVYEGLTNYLG
                     YVLSARSGLWSADMGRQAWASVAASMDRDRSGRAWRDLEDTTEQPIITARRPLSWLSW
                     QRTEDYYTEGQLLWLDVDTRIRELTADKRSLDDFIRAFYGPAVRVPQERTQGTTAVSA
                     NSLPKVTLGPVTYTFADVVQALNAVARYDWESYFRQRLYEHASGAPLEGLTRGGWKLV
                     YTGTPSEYTKSSEEQRKVLDFLYSLGFDISAQNAQLTEVRWGSPAYAAGLTIGTTLIA
                     VDGREYAPERLTNAIVMAGTTRRPIELLVKNLDRYRTVSINYTEGLQYPQLQRLERTK
                     DRLDAIMKPRT"
     gene            complement(5853..>8009)
                     /gene="putA"
                     /locus_tag="VFB37_12445"
     CDS             complement(5853..>8009)
                     /gene="putA"
                     /locus_tag="VFB37_12445"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HZO23303.1"
                     /translation="SRRKITVRLVKGAYWDSEIKRAQERGLASFPVFTGKPATDACYL
                     VCTQRLFAARDVIYPQFATHNAFTVAAVLELAPAGAQYEFQRLHGMGELVFDAVRHEV
                     PGLRPVRTYAPVGTHEDLLPYLVRRLLENGANTSFVHQFLNPQVPVEQVVRDPLSALG
                     EASQPSLAATEGQRAPSLASAESDAAHRIREPEALYAPERRNSPGVDWGDPKELQALT
                     AEIGKLAAQMHKGGPLLGGTPAHELEVPVISPANTLETVGLTRDATEDEINRAMDAAA
                     QAQPEWDALSAIERAACLDRAADLLEQRRAAFLSLLVREAGKTLPDAVAELREAIDFC
                     RYYAARGRELFGVQQLQGPTGEKNLLSLHGRGVFVCISPWNFPLAIFTGQITAALMAG
                     NSVVAKPAPATPLVAHAMTALLHEAGVPGNVLQLTPADGPPFGNVVFKHAALSGVAFT
                     GSTATAATINRALAHREGPIVPLIAETGGVNAMIVDSTALPEQVVDDVVTSAFTSAGQ
                     RCSALRVLYLQEDVADRIIEMLVGAMKCLVIGDPSHPETDVGPVISDAALARLRAHTA
                     RMGQQAHLLYACPLETHHGLGHFFAPHLFELRALDQLKTEEFGPILHVVRYRSEQLPQ
                     VIAAIRSTGFGLTLGVHSRLESLADHVFRSLPVGNTYVNRNMIGAVVGVQPFGGQGLS
                     GTGPKAGGPHYLLRFANERTLTINTVAVGGNVELLS"
BASE COUNT         1396 a         2580 c         2609 g         1424 t
ORIGIN      
        1 gggccggcgc cgtcggagtt ctcctcgcgt cgcatgggcg tcggggcggc ggtcgcaccg
       61 aaatccatca cgggtgcggt tcgcgtgcgc accgcatcga gtccgccatg caggtcgatc
      121 gggctcgtgc tcgccccgcc cgggcccgcc tgtccgggtg ccggcttcga agtcttgccc
      181 ggctccatgc tcggattctt gaagtaatcg aaaattgcgg cgcgctgatc cttggagacg
      241 gcggtcacga gccacatgac gaggaagaac gccatcatgg cagtcacgaa atcagcgtag
      301 gccaccttcc aggcgccgcc atgatgacca tgcccttgac gccctttgcg cttgacgatg
      361 atgagtggcc gttcagcctt ggatttgggc gcagccatgg cgaagcggct ctaggcggct
      421 ttcgccttgg ccttgagctg cgtctccaga tcgctgaact tcggccggat gtcctgtgag
      481 agcagcttgc gagcgaactc gatggccacg gcgggcacgt agccgcggac ggcggccacc
      541 agcaccatct tcaccacgct gaacgccttg ccttcctcgt tggccgcgtg ctccatcgcg
      601 gcggcgatgg ggcctaccag accgtaggag acgagaatgc cgaggaaagt gcccacgagg
      661 gcagcgccca ccttgtggcc gatcgaggct gtgtccgcgc catccagcgc ggccatggtg
      721 ttcacgatgc ccagcacggc cgcgacgatg ccgaagccgg gcaatgcatc ggccaccttc
      781 tgcaccgcct gagcgggctc gtgggcttcc tggtgatgcg actcgagctc ctgctccagc
      841 aggctctcga gctcgtgcgg atccagattg ccgccgatca tgagccgcag gcagtcgcgg
      901 atgaaatcca tcaggtggtg atcggccacg atcttcggat aattgcgaaa caacgccccg
      961 ccgtcctcgg cctccaggtc tccctcgatg gccatcagac cttccttgcg gatcttcacc
     1021 agcacgtcat acagacactt cagcagctcc acgtagtcat cacgcttgta gcggggacct
     1081 ttgaccaggc cgagcgcacc catgaaggct gccttggtca ccttgggtgg attgctggtc
     1141 aggaaggctc cgaatgccga gccgacgatg atcaggacct cggtcgggtg ccagagggca
     1201 agcaacttgc cgccctcgag cacgaacccg gcgatcacgc agccgacgac gacgatggaa
     1261 ccgactatct ggatcacggg atggctggcc gttactcgtg tggcggaagg ggcgatccgt
     1321 cgcaccgggt gaccctgtaa cggcggattc ctgtttacct ttactgcaaa cctgtgcagt
     1381 acccgcatca ttgccgcagg tgtgaggcgg gcgtcgtgac gcaactctca cgatgacagc
     1441 agcgccgggc agggagcgct gcggctttca ccggtttgag cggtaccggc cacaatcgag
     1501 cgtggttgct gcgccgcaac agcggggcac tgtgcgcacc gggtccgtgc aggagcgcct
     1561 ccgctgcact ctcgtcgagc gttcctttgc tgcgatccct cctgcatgag ccgccgaagg
     1621 cgctgaaacg cgcactgcgg atcatcggtc acgactggca cggctgttgc tcaaagcccg
     1681 ctcgtgattt gtgtttcgaa ggcaggccgc ccgccggagg tcctgatgag tgcgcatgtt
     1741 ctgacaccag ccgatcggga cgaggcggct gcgaagccga tcgaactcac gcgaccggtg
     1801 cgtgagcctg agatcatcga tgccgcagca agagtgatca cccgccgcag tgatcatcga
     1861 attctctgga gcacgatcgc tcacgaagcc ggccactgcg cagcacccat cgaaacatgc
     1921 ttcgacggct tgctcacgct catcgacgcg tgttactcgc gcacggcgca aggcctgagc
     1981 catagtctgc ttcaggccga aaccgccccc ggcaccgcgc tggaccaagt cgcggcattc
     2041 ctcgtctctg ccctggagat cagacgctcg cgcggtgtgt tcctgtcctt caggcgatgc
     2101 gcagatctgc cgggatcctt gcagcgacgg ctgcacgagt acgatgccat gacgcggact
     2161 cgactgaagc ggttgctcca gcgagggcac cgcgacggct cgctggcaca tcgccagccc
     2221 gacgcggcgg tggagctgat actggcgtgc cttcaggtgc ccaccgtcgt cacggacggg
     2281 cccgagcaaa ggatgtggga cggcgagctc gtggagctcc tgctcgcggg cctttccgag
     2341 ccgcatcctc ccgaggacgg cacccgactg ctcggctgag gcgcccgcgc gggattgggt
     2401 ttcacccgac cgcggtggcg gtgctggcga gccggccaac cgtgcccgac cggtgagccg
     2461 agcgggtaca ctggaccttc cggcgcctgc tgtagcgccg agccgtgagg tcgatgatgt
     2521 tcgagcgcct gcatcgcctg cagaccgatc cgattctggg tctgatggcc gccttccggg
     2581 cggacacgag cccccgtaag gtcgatctcg gggtcggggt gtaccgggac gatcacgggg
     2641 aaacacctgt cctgggctgc gtaaggcgcg ctgaggaggc ggttctcgcc cggcagtcga
     2701 ccaagtccta cgtgtcctca gtgggcaatg tcggtttcaa tcaggccatg gagcggctgg
     2761 ttttcggcga gagccatgcg gctttgagct ccgaccgcgt gcgaaccatc caggctcctg
     2821 gtggatgcgg ggcgctgcgc ctgggcgcgg aactgatccg caatgcatgg cccgataccg
     2881 ttatccatgt cagcactccg acctgggcga atcatgttcc cctgctgtca ggcgcgggtc
     2941 tgaagctgga gcgctacccg tattacgatc cggccagcgg aggggtccgg ttcgagtcca
     3001 tgctcgccgc actggaaaca ctcccggcgc acgcggtcgt actgttgcat gcctcctgtc
     3061 ataaccccac cggggcggat ctgagcgaag cgcagtggcg cgagctgctg agtgtgatca
     3121 gacggcgaac gcttttgccg ttcatcgaca tggcctatca gggtctcggt tccggaaccg
     3181 cggaggacgc cttcgccatc cgtctcttct gtgaagagct gcccgaggtg gccgtagccg
     3241 tgtcgtgctc gaagaacttc ggactgtacc gggagcggac cgggtgcctg cacgtactca
     3301 gcgactcggc gaaatccggt gatgccgccc tgagtcacct ggtgcgaatc gcgcggagtc
     3361 tgtattccat gccgcccgat cacggcgccg caatcgtgca cgagatcctg gggaacccgg
     3421 cattgtggca ggagtggacc ggggaggttg gcgggatgcg cgcgcgactg accggtctgc
     3481 gacaggagct ggtcaagcat ctcggccggg cctgtccaca gcgcgacttc catttcatcg
     3541 ccgcgcagcg tggcatgttc tctttcctgg gtgtcgatac cgcacaggtg catgccctgc
     3601 gcgagcgaca tcacatctac atgaccgatg acagccggat caacatcggc ggattgcgcg
     3661 cggacaacct ggaatacttt gcccaggccg tcgcgcaggt gctcggctga acggaagacc
     3721 tgcgccgaac ccactccgag gagctcatga aacgattcgt gctggtggtg atcagcgccg
     3781 ttgtgtcggt gttgtccatc gtgaacgcgc aggaggcagg caaggagacc gcgcctgcgg
     3841 tggaagcgcc cggcaccact gccgcgcagg cggcaacccc gctcaacccg gagagggcta
     3901 cagcgggcgt ggatcgctcc gggacatcgc gaggccgggt gccggtaccg cagaacgtcc
     3961 cctatccggg aaccctgacg ctcaaagttg atttgacgga tctggaccgc cgcgtgttca
     4021 ccgtgcggga aagcattccg gtgaagcccg gaccgctcac tctgttgtac cccgagtggc
     4081 tgcccggcaa tcatgcgccg cggggcccga tcgatgctct cgcaggtctg accctcatgg
     4141 ccgcggatca tcggatcgaa tggactcgcg atgccgtcaa cgtctatgcc tttcacctgg
     4201 atgtgccggc gggggcgaat cgtctggacg tgcagttcca gttcgcctcg ccctttctgc
     4261 acgagcaggg gcgcaccgtg gtcacccccg atatcgtggg cctgcagtgg aacacggtgg
     4321 tgctctatcc ggcaggctac tactcgagcc ggatctcgat gctcccctcg gtcgttctgc
     4381 ccgccggctg ggccttcgct tctgcgctcg atgtggacag tcgcggcggc aaccaggtca
     4441 gtttcagacc gacgacgctg gagatgctgg tggactcgcc gttgttcgcc ggtcactact
     4501 tcaggagcgt ggatctggac cccggagctg cggtgcctgt ccacctcaac atggtcgccg
     4561 acagcccgga gagtctggag atcaaggggc cggagctggt cgcgcatcgc aagcttgtgc
     4621 aggaggctta cgccctgttc ggggggccgc actacgatca ctacgagttt cttctggcgc
     4681 tcagcgatca tttcggcgag atcggcctcg agcaccatcg ctccagcgag aaccgccggc
     4741 cgccgggtta cttcacggac tgggacaagt cgggcgcgct ccgcgatctg ttgccacatg
     4801 aattcagtca ttcctggaac ggcaagttcc gccgacccga aggcctcgcc acgcccgact
     4861 tcaacacccc gatgcgagac tcactgcttt gggtctacga gggtctgacc aactatctgg
     4921 gctacgtgct gtcggcgcgc tccggcctgt ggagtgcgga catgggccgt caggcctggg
     4981 cgagcgtcgc agccagcatg gaccgcgacc ggtccggccg cgcctggcgg gatctggagg
     5041 acaccacgga gcagccaatc atcaccgccc gccgtcccct gtcctggctt tcctggcaac
     5101 ggaccgagga ttactacact gaaggacagc ttctgtggct cgacgtggac acccggatcc
     5161 gcgagctcac cgcagacaag cgctcgctgg atgacttcat tcgcgcgttc tatgggcctg
     5221 ccgtacgcgt tcctcaggag agaactcaag gtacgactgc cgtgagcgca aacagcctgc
     5281 ccaaggtgac gctgggcccc gtgacctaca cgtttgcgga tgtggtccag gcgctgaatg
     5341 ccgtggcgag gtacgactgg gaaagctact tcaggcagag gctttatgag cacgcttcgg
     5401 gtgcgccgct cgagggtctg acgcgaggcg gctggaagct cgtctacacg gggacgccat
     5461 cggaatacac gaaaagctcg gaggagcagc gcaaggtgct cgactttctc tactcgctcg
     5521 gattcgacat ctcggcgcag aacgctcagc tcaccgaagt ccgctgggga agccccgctt
     5581 acgctgccgg cctgacgatc ggaaccacgc tcattgccgt cgatggccgc gagtacgcac
     5641 ccgagcgcct gaccaacgcg atcgtgatgg ccggcaccac caggcgccct atcgagctgc
     5701 tggtcaagaa tctcgatcgc taccggacgg tgagcatcaa ctacaccgag ggcctgcagt
     5761 atccacagct gcagcgcctc gaacggacca aggatcggct ggatgcgatc atgaagccgc
     5821 ggacctgaga gcggcgccgg tccggggtca ccttaggaaa gcaactcgac gttcccgccc
     5881 acggccacgg tgttgatcgt cagcgtgcgc tcgttcgcga accgcaacag gtaatgcggg
     5941 ccgccggcct tgggtccggt gccggacagg ccctgtccgc cgaagggctg tacgccgacc
     6001 actgcgccga tcatgttccg gttcacgtag gtgttgccca ccggcagcga gcggaaaaca
     6061 tgatcggcca gactctcgag cctgctgtgc acgccgagtg tcaggccgaa tccggtgctg
     6121 cggattgcgg cgatgacctg cggcaactgc tcggatcgat agcgcaccac gtgcaggatg
     6181 ggcccgaatt cctccgtctt gagctgatcc agtgcgcgca gctcgaacag atggggtgcg
     6241 aagaagtgcc cgagcccgtg atgggtttcg agcggacagg cgtacagcaa gtgtgcttgc
     6301 tggcccattc gagcggtgtg cgctctcaat cgtgccagcg cggcgtccga gatcactgga
     6361 ccgacgtcgg tctcgggatg ggagggatcg ccgatcacga ggcacttcat cgcgccgacc
     6421 agcatctcga tgatgcgatc cgccacatct tcctgcagat acagcacacg cagcgcggag
     6481 cagcgctgac ctgcgctggt gaaagccgat gtcacgacat cgtccaccac ctgctccggc
     6541 agtgcggtgg aatctacgat catggcattc acaccgccgg tctccgcgat cagcggcacg
     6601 atggggccct cgcgatgggc gagcgcacga ttgatcgtgg cggcggtggc ggtggacccc
     6661 gtgaatgcca cgcccgagag cgcagcatgc ttgaacacca catttccgaa gggcggccca
     6721 tcggcgggtg tcagttgcag aacgtttccg ggcacgccgg cttcgtgcag gagtgccgtc
     6781 atcgcatgag cgaccagcgg agttgcgggc gcaggcttcg cgaccacgct gttgccggcc
     6841 atgagcgccg cagtgatctg acctgtgaag attgccagcg gaaaattcca cgggctgatg
     6901 cagacgaaca caccgcggcc atgcagggac aggaggttct tctcacccgt gggtccctgc
     6961 aactgctgca cgccaaaaag ctcacggccg cgcgcggcgt aatagcggca gaagtcgatc
     7021 gcctcgcgca actccgcaac tgcatccggc agcgtcttgc ctgcctcgcg aacgaggaga
     7081 ctcaggaacg ccgctcggcg ctgctcgaga agatcagcgg cacggtcgag gcaggcagcg
     7141 cgctcgatgg ctgacagcgc gtcccactcc ggctgggcct gcgcagcggc gtccatggcg
     7201 cgattgatct catcctcggt cgcatcacgc gtcaggccaa cggtttccag ggtattcgcg
     7261 ggcgagatga ccggcacttc gagctcgtgc gcgggtgtgc cgcccagcag gggccccccc
     7321 ttgtgcattt gggcggccag tttgccgatc tccgccgtca gtgcctgcaa ctccttcgga
     7381 tccccccagt ccactccggg cgagttgcga cgctcggggg cgtagagcgc ctcgggctcg
     7441 cggatgcgat gagccgcatc ggactctgca gacgcaaggc tcggtgcacg ctggccttcg
     7501 gtggcggcga gactcggttg cgatgcttcg cccagagctg acaagggatc tctcacgacc
     7561 tgctccacgg gaacctgcgg gttgaggaac tgatgaacga aggacgtatt ggcgccgttc
     7621 tccagcaggc gccgcacgag gtaaggcagg agatcctcat gcgtgccgac tggcgcgtaa
     7681 gttctcacgg gacgcaagcc cggcacctcg tgccggaccg catcgaacac gagctcgccc
     7741 atgccgtgca gtcgctgaaa ttcgtactgc gctccggcag gagccaactc gagcaccgca
     7801 gcgacggtga atgcattgtg ggtggcgaac tgcgggtaga tgacgtcgcg cgctgcgaag
     7861 agccgctgcg tgcacacgag ataacacgca tccgtagcgg gcttgccggt gaataccggg
     7921 aagctggcga gaccacgctc ctgcgcgcgc ttgatctcac tgtcccaata ggctcccttg
     7981 accaggcgca cggtgatctt tcggcggct
//
LOCUS       DASRHN010000316         5909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_272873, whole genome shotgun sequence.
ACCESSION   DASRHN010000316 DASRHN010000000
VERSION     DASRHN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272873"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFB37_12450"
     CDS             complement(<1..238)
                     /locus_tag="VFB37_12450"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZO23304.1"
                     /translation="MTDSPSADLARLAELIREIGIALLTTVDEQGLLSTRPVQTMEYE
                     SEGVLWFFTDWHSPKANELRQDRRVSLGYANPARH"
     gene            complement(281..1615)
                     /locus_tag="VFB37_12455"
     CDS             complement(281..1615)
                     /locus_tag="VFB37_12455"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZO23305.1"
                     /translation="MSSHSISRRDLLATLLCACASGSFARAAATTPSKDKLTVDAARL
                     QRTLEELSTFGRPAGASFAEGVSRVAYSDADIAGRAYVIAQMKAAGLDLRVDPAGNII
                     GRRAGSGHALKPILFGSHIDSVPSGGNFDGDLGSLSALEVIRRLNEERITTRHPLEVT
                     IWSNEEGGTIGSLAVIGGLSPTILNWESNSVRIADGLRRIGGDPDRLEEARRAPGSIH
                     CYLELHIEQGGLLDKAGVPIGVVDGIVSIDEYEVEVRGFANHAGTTPMPERRNALLAA
                     AKIIEAVQEEVTRMPGRQVGTVGHLEVYPNTRNVIPGLVKHSIELRDLSEEKLATLGA
                     AIQQRIPPIARETQTEISVRHVTHDPAALATPAIQREIEAAASTLGLRTLHLPSGAGH
                     DAQVLAKVGPMGMIFVPSVAGISHSPHELSRWADCANGANVLLQTVMRMDRS"
     gene            complement(1633..3966)
                     /locus_tag="VFB37_12460"
     CDS             complement(1633..3966)
                     /locus_tag="VFB37_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO23306.1"
                     /translation="MNTTDSTPRFNAPQARELVLEHFGLSVSARPLPSERDQNFALEC
                     ASGAKLVLKIAKSDEDPAVLDFQNAALRHAHERLAVLAIPQVLPSKSGQEITRISDRA
                     GRAYWLRLVSWLDGELLSAIGQRSPKLLASLGRCLAELDAALQDFHHPLMHRQLHWDL
                     RHIGLALAHLDLLDAEQRGIVGHFRGLWESVPWQELRSSVIHGDINDNNVLARNGVVC
                     GFIDFGDLVHSALIGDLAIAIAYAMLDQQDPLGVAAVVTAAYNERFPLSGPEIAALYP
                     LVICRLCMSVCYAAWSGIVKKGDAYQTVHQDSAWRILRRLRAIPLEEAEAAIRAACGG
                     TARLLAARRRLFGGSLSLSYRQPLNLVRGSMQYLYDQDGRRFLDGYNNVPHVGHCHPE
                     VVRAAHTQMALLNTNTRYLSELAVRYAEELVTTLPGALKVCFLVNSGSEANELAIRLA
                     RSHTRQRDIIVLEQAYHGNTNTLVDLSPYKHVGPGGEGAPSWVHTAELPDLYRGRYKR
                     QDPEAGGGYAQSVLRLLSDLHDAGRAPAAFIAESWPSVAGQMDLPADYLPAVYRAVRA
                     AGGVCIADEVQTGYGRLGAHFWGFQAYGVEPDIAVLGKPIGNGHPLGAVVTTAEIAAS
                     FDNGMEFFSTFGGNTVSCAIGRAVLGVVLQEGLQAHARAIGDQLLAALRTLMARHAII
                     GDVRGSGLFLGVELVRDRHTLEPAAEEASIVVNRLREQGVLIGTEGPHENVLKIRPPM
                     PFNQEDAGMLISALEDALRPLDSHARAESRAPASIRR"
     gene            complement(3963..>5909)
                     /locus_tag="VFB37_12465"
     CDS             complement(3963..>5909)
                     /locus_tag="VFB37_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO23307.1"
                     /translation="SLLVAAWILDLAGLSLNTMTIGGLVVALGVVVDDAVIDVENILR
                     RRRNAAHDLDVMSLIMAASLEVRRPVFYATMAVVVAFVPILLLSGLQGAFFSPLSVAF
                     LLAVGVSLLVAMSVTPALCVIVMRHHSPVQEAHWLRRLKSFQQRSIEWLTRRPRLAWT
                     ILVITALIGVGLLPLLGARLLPDFRESYLIVHTILRPGVSIGETERMDARISARLAAI
                     RGVRGVAAQIGRAENGQDPDTPNKSEFEVQVDPAAGRTVVEQIRAAFEDFPGQLSEIN
                     SMLTERIGETLSGDAAPFLVSVFGTDLEADDRIAEQVAKVLRSLPSSGAVRLLVPVRQ
                     PEIELALKPEQLSAHGLQAGDVLEAVSASFSGSSVSQISLADRSIPVVIRGKVETSTP
                     EAVKNLLLRGRDGNLVELSSVADVELIQGRALLNHEGGLRRQVVMASPQGKDQAGFAE
                     TARRAITARVQLPRGIYLGFGGSAVEQASALRELLFHSSAAIVLIVLLLAIAFGRSRQ
                     VLLVIMALPSTLIGGVAALALADASLTIGATVGFVALFGMAARNSILLVSHYDFLTMH
                     EGHSWGRLTALRGAEERFTPVLLTALLTALALLPVALQPGKPGHEIEGPMAIVILGGL
                     VSSTLTTLFIIPALSVRWLANRPA"
BASE COUNT         1067 a         1979 c         1876 g          987 t
ORIGIN      
        1 tgtgccgggc cggattcgcg taaccgagac ttacgcgccg gtcctgcctg agctcgttcg
       61 ctttcgggct gtgccagtcg gtgaagaacc agagcacgcc ctcggactca tactccatgg
      121 tctgcacggg gcgggtactc agcaggccct gctcatcgac ggtagtgagg agcgcgatgc
      181 cgatctcgcg gatcaactct gcgagcctgg ccagatcagc ggaaggggaa tccgtcacgc
      241 gtggatcgga gcaaggttca tgcccctcac agcgaccggc tcaactccgg tccattcgca
      301 tgacggtctg cagcagcaca ttggcaccgt tggcacaatc ggcccacctg gacagctcgt
      361 gcggcgaatg gctgatcccg gccacgctgg gcacgaagat catccccatg ggacccacct
      421 tcgcgagcac ctgcgcgtcg tggccagccc cgctcggcag gtgcagcgtg cgcaaaccga
      481 gagtgctcgc ggccgcttcg atctcgcgct ggatggcagg tgtcgccagg gccgccgggt
      541 catgcgtgac gtgccggacc gagatttcgg tctgagtctc ccgggcgatg ggcggaatgc
      601 gctgctggat ggcggcgccc aatgtggcca gcttctcctc ggaaaggtcc cgcaactcta
      661 tcgaatgctt caccagtccg ggtatcacat tgcgggtgtt cggatacacc tcgagatgtc
      721 ctacggtccc cacctgtctg cccggcatgc gcgttacctc ctcctggacc gcctcgatga
      781 tctttgcggc ggcgagcaag gcgttacgcc tctcgggcat tggcgtcgtg ccggcatgat
      841 ttgcgaagcc gcggacttcg acctcgtact cgtcgatgct gacgattccg tccacgacac
      901 cgatcggaac gcccgccttg tccagcaagc cgccctgctc gatatgcagc tcgagataac
      961 aatggatgga cccgggcgcg cggcgcgctt cctccaaccg gtccgggtcg cccccaatgc
     1021 ggcgcaagcc atcggcaatg cgcacgctgt tgctttccca gttgagtatg gtgggcgaca
     1081 ggccaccgat caccgccaga ctgccgatgg ttccgccctc ctcgttggac cagatcgtga
     1141 cttcgagcgg gtggcgcgtg gtgatgcgct cttcgttcag tcgtcgaatg acctccaagg
     1201 ccgacaggga gcccagatcc ccgtcgaagt tgccgccgct cggcaccgag tcgatgtgtg
     1261 atccgaacag aatcggcttc agtgcgtgtc cgcttcctgc ccgtctgccg atgatgttgc
     1321 ccgccggatc aacccgcagg tcgagccctg cggctttcat ctgtgcgatc acgtacgcgc
     1381 gcccggcgat gtcagcatcc gaataagcta cgcggctcac gccctcggca aagctcgctc
     1441 ccgccggtct gccgaacgtg ctgagctcct cgagcgtgcg ctgcaggcgc gcggcatcga
     1501 ccgttaactt gtcctttgaa ggcgtggtgg ccgctgcacg ggcgaacgat ccgctggcac
     1561 aagcgcacag cagcgtagcc agaagatccc tgcggctgat cgaatgactc gacatacaac
     1621 cctcccgcga gctcagcgcc tgatggacgc aggtgcgcga gactctgcgc gcgcatgcga
     1681 atcgagcggc ctcaaagcat cctccagggc gctgatcaac atcccggcgt cctcctgatt
     1741 gaagggcatg ggtgggcgga tcttgagcac gttctcgtga gggccttcgg tgccaatcag
     1801 caccccttgc tcgcgcagtc ggttcacgac gatcgatgct tcctctgccg ctggctcgag
     1861 cgtatgccgg tcgcgaacga gctcaacccc gaggaacagg cccgagccgc gcacgtcccc
     1921 gatgatcgcg tgccgggcca tgagggtgcg cagagccgcg agcagctgat cgccgatcgc
     1981 tcgggcatgc gcctgcaagc cttcctgcag cacgacaccc agtaccgccc ggccgatcgc
     2041 acacgatacg gtattgccgc cgaacgtgct gaagaactcc atgccgttat cgaacgaagc
     2101 cgcgatttca gccgtcgtca cgaccgcgcc caggggatgg ccgttgccga tgggcttgcc
     2161 gagcacggcg atgtccggct cgacgccgta cgcctgaaaa ccccagaaat gagcgcccag
     2221 ccgtccatag ccggtctgga cttcatcggc gatacacact ccgcctgctg cgcgcaccgc
     2281 ccgatacacc gcgggcagat agtctgccgg cagatccatc tggcctgcga cgctgggcca
     2341 ggactccgcg atgaaggcgg caggggcccg tcccgcatcg tgcaggtccg acagcagccg
     2401 cagcaccgac tgcgcatacc cgccgcccgc ttccggatcc tggcgcttgt agcggccgcg
     2461 atagagatca ggcagctccg cggtgtgcac ccatgagggg gcgccctcgc cacctggtcc
     2521 aacgtgcttg taaggactga ggtcaacgag cgtgttggtg ttgccgtgat aggcctgctc
     2581 gagcacgatg atgtcgcgct gacgcgtgtg ggagcgggcg agtcggatcg ccagctcgtt
     2641 cgcctcgctt cccgaattca ccaggaagca gaccttcaga gcacccggca gggtagtgac
     2701 cagttcttcc gcatagcgga ccgccaactc gctcagatag cgggtgttgg tgttgagaag
     2761 cgccatctgg gtgtgcgcgg cgcgcacgac ctccggatgg caatggccca cgtgcggcac
     2821 attgttatag ccatccagaa agcgccgtcc gtcctgatcg tacagatact gcatcgagcc
     2881 gcgcacgaga ttcagcggct gccgatagga aagactcagg cttccaccga acaggcggcg
     2941 gcgcgcggcc agcagccgcg ccgttccgcc gcaggcggct cggatggcag cctcggcctc
     3001 ctccagggga atcgcgcgaa gtcgcctgag gattcgccac gcggaatcct ggtgaacggt
     3061 ctggtaggcg tcgcctttct tgacgatccc actccaggcc gcgtagcaca cgctcatgca
     3121 cagccggcag atcacgagcg gatacagtgc ggcgatctcg ggcccgctga gcggaaagcg
     3181 ctcgttgtaa gccgccgtca ccaccgcggc cacccccagc gggtcctgct gatcgagcat
     3241 cgcataagcg atcgcgatgg ccagatcacc gatcagggct gaatggacca ggtcgccgaa
     3301 gtcgatgaag ccgcagacga cgccgtttcg cgccagcacg ttgttatcgt tgatatcccc
     3361 gtgaatgacg ctggatcgca gctcctgcca cggcacgctc tcccacaagc cgcgaaagtg
     3421 gccaacgatg ccgcgctgtt ccgcgtccag aagatccaga tgcgccagag cgaggccgat
     3481 gtggcgcagg tcccagtgca gctggcggtg catcagcgga tggtgaaagt cctgcagggc
     3541 ggcatcgagc tccgcaaggc agcgcccgag ggaggccaga agcttgggac tgcgctgccc
     3601 gatcgccgag agaagctcgc catcgagcca gctgacgagt ctcaaccagt aagccctgcc
     3661 cgcccgatca ctgatgcgcg tgatctcctg ccccgacttg gagggcagca cctgcgggat
     3721 ggccagtacg gcgagtctct cgtgagcgtg cctgagtgcc gcgttctgga agtccagcac
     3781 ggccggatcc tcgtccgact ttgcgatctt gagaacgagc tttgcgccgc tggcacattc
     3841 gagcgcgaag ttctgatctc gctcactcgg caacgggcgc gccgacacgc tcaggccgaa
     3901 gtgctcgagc acgagctccc gggcttgcgg ggcattgaat cgcggagtgg agtcggtcgt
     3961 gttcatgctg ggcggttcgc aagccatcgt accgagagcg ccgggatgat gaaaagcgtg
     4021 gtgagcgtcg aggagaccag cccgccgagg atcacgatgg ccatgggccc ttcgatctca
     4081 tgtcccggct tgccgggctg cagcgccacc ggcagaagcg cgagggcggt gagcagcgcc
     4141 gtgagcagca ccggggtgaa tcgctcctcg gccccgcgca aggcggtgag ccggccccaa
     4201 gagtggcctt catgcatcgt gaggaaatca tagtgcgaga ccagcaggat cgagtttcgc
     4261 gcggccatgc cgaacagggc gacgaatccg accgtcgccc ctatcgtcag tgaggcgtcc
     4321 gccagcgcca gcgcagccac tccgccaatg agcgtgctgg gcaaggccat gatgaccagc
     4381 agcacctggc gcgatcttcc aaacgcaatg gccagcagga gcacgatcag tacgatcgca
     4441 gcgctggagt gaaacaggag ctcacgcagc gcactggcct gctccactgc cgagccgccg
     4501 aagcccagat agatgccgcg cggcaactgc acgcgcgcgg tgatggccct gcgcgcagtc
     4561 tcggcgaatc ctgcctggtc cttgccctgc gggctcgcca tgacgacctg ccgacgcaac
     4621 ccaccttcat gattgagaag cgcccggccc tggatgagtt cgacgtctgc aacgctcgac
     4681 agctccacga ggttgccatc ccgtccgcgc agcagcaggt tcttcacggc ctccggtgtc
     4741 gaggtctcga ctttcccgcg aatcaccacg gggatgcttc gatcggccag actgatctgt
     4801 gaaacggagc tgccgctgaa ggatgcactg accgcttcga gcacatcgcc ggcctgcagg
     4861 ccatgggcgc tcagctgctc aggtttgagg gccagctcga tttccggctg gcggaccggc
     4921 accagcagcc gcacggcgcc gctcgatggc aggcttctga gtaccttcgc gacctgctct
     4981 gcaatccggt catcggcctc cagatcggtc ccgaagacgc tcacgagaaa tggagccgca
     5041 tcgcctgaca gcgtctcgcc gatccgctcg gtgagcatcg aattgatctc cgaaagctgg
     5101 ccggggaaat cctcgaacgc tgcccggatc tgctcgacca ccgtgcggcc ggcggccgga
     5161 tcgacctgga cctcgaactc gctcttgttg ggcgtgtccg gatcctggcc gttctcggcg
     5221 cgaccgatct gcgccgccac gccccgcacg ccccggatcg ctgcgaggcg cgccgagatg
     5281 cgcgcatcca tccgttcggt ctcaccgatg gacaccccag gccgcagaat cgtgtgaacg
     5341 atcaggtagc tctcccgaaa gtccggcagc agcctcgcac cgagcagggg cagcagcccg
     5401 acgccgatga gggctgtgat caccaggatg gtccaggcga gccgggggcg ccgggtcagc
     5461 cactcgatcg agcgctgttg aaagctcttc agccgtcgca gccagtgcgc ttcctgcacc
     5521 gggctgtggt ggcgcatcac gatcacgcac agtgccggcg tcacactcat cgcgaccagc
     5581 agcgagacac cgactgccag cagaaaggcg actgacaacg ggctaaagaa cgctccctgc
     5641 aggccggaca gcagcagaat gggcacgaag gcgacgacca cggccatggt tgcgtagaac
     5701 accgggcgcc tcacttcgag cgaggcggcc atgatgaggc tcatgacatc gaggtcgtgc
     5761 gccgcgtttc gccgccgccg caggatgttt tccacatcga tcacggcatc gtccaccacg
     5821 actccgagag cgaccacgag ccctccgatg gtcatggtgt tcaggctgag cccggccaga
     5881 tcgaggatcc aggcggcaac cagcagcga
//
LOCUS       DASRHN010000317         8172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_602555, whole genome shotgun sequence.
ACCESSION   DASRHN010000317 DASRHN010000000
VERSION     DASRHN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602555"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..610)
                     /locus_tag="VFB37_12470"
     CDS             complement(176..610)
                     /locus_tag="VFB37_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZO23308.1"
                     /translation="MRNLSIGAVARECGVASSALRFYEKEGLLPAPARKSGQRCYDEG
                     VFARVEIIKLALEAGFSIAETRIFLSGFSKETPPAARWRALATRKLEELNALMDRTQQ
                     MRSLLESSFHCGCPSLEDCEKFLRARRRAGQSAARAAKPAGK"
     gene            706..1479
                     /locus_tag="VFB37_12475"
     CDS             706..1479
                     /locus_tag="VFB37_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23309.1"
                     /translation="MLHLLKRTRAIPGILILLAALAARAEASVPDYIAAAVADPARPL
                     QDQARDRERKAAEVLAFSGIGPGARVADFMSGGGYFTRLMSRIVGDTGKVYAFLPEEE
                     LRNCVPQETAGTLALEHDARYANVSVLRSPVKQFHAPEPLDLVWTSLNFHDLYDAFME
                     HADVPAVTQALYRALKPDGVLLVIDHAAQPHSGIRDTEKLHRIDPQVIISAALAAGFR
                     LDARSDLLRNPADTHQLLVFDPRIRGRTDQVILKFRKPR"
     gene            complement(1463..3763)
                     /locus_tag="VFB37_12480"
     CDS             complement(1463..3763)
                     /locus_tag="VFB37_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO23310.1"
                     /translation="MSVPVSPKMVQFFTRVIQARVWIAGCFLALFAVGIYGALQVRDD
                     PAIDRLVVAADPVARATADFDRLYPEGDQALLMLEAPDPLSLRSLQAAQQIESELGRI
                     PHVEAHSLLTLYRRANGERELDAAEIARVRAFATGTTLFRRAGLLGEHYLGVALELRV
                     ATPAERDRTLAAIDSVLRPLGSGAGPFTAIRRVGSPWLDAWLEQRTGAATARFMPLFG
                     IFLMALVFIVYRSWRALGAIIVTLAVVVAIAVGLADLFGWSHTVISTLVPLTVMVTTT
                     ATLVYLHARYMEPDDCANPLEHHARALTNKFLPCTASMFATAVGFGALAVSDIQPVRE
                     MGLWTASGLIVAWVACFTLFPALQSLLRTPLRTERAAAGKWFPGLVQALVPATRRYRW
                     PFVGAALALMLCGAGALFGIPGLLTPLSLQTDVLTYVNPHERVAQDTRTFQQSNGLDV
                     IELWVQTTPGHALDPELLRGLERFTHQLESDPRITAVDGPTSTLRWERYVQSGSDRLP
                     SAPDAWPKLAADLEQIMLTEPAARAYVDVGNLASVRISIRGRADLFGRTGAMRRYVEQ
                     TWLAVQAAEPALRSAHALAVGRGVLGTEITERLLPTLTESFAITASLIFLAFLLVFRD
                     PSARLMTMIPSLFAILAVFLIMRLGGIALNIATILIGSTVLGATENDQIHFFYHFQEG
                     SASGSTAEALRHAMQVAGRPILFATLINSSGFLALALSDLPPMRQFGIVSSSAFILAL
                     LADFTALPGALWILSRGKRRVLTEAS"
     gene            3826..4599
                     /locus_tag="VFB37_12485"
     CDS             3826..4599
                     /locus_tag="VFB37_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23311.1"
                     /translation="MSSLLTAAIATLVFCAPGYPGAAGDAQPFIDQFVSAATANAGWP
                     KGSLAAVYDPSEAGGLTKLTSPDAVLAFIPYPFFVEHAAQLHLTPLAQADVTGLGTTE
                     RWTLVGKSGLPDRPQALNGYTILSVAGYSPVFVRQSALGAWTLPQGVRVESTGQILSA
                     LRRVASGETVVALLDQTQAAAIPTLPFAAQLTPMLQSAPVPVALLAIVDSRLTPARAH
                     ALQGALLKMGHAGSDADVLGALHLQRFVAPQLPASRIGP"
     gene            4596..5309
                     /locus_tag="VFB37_12490"
     CDS             4596..5309
                     /locus_tag="VFB37_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23312.1"
                     /translation="MSGRRAAAAALALAIAACTTTPRPADRAPEHPQNASVIELSGAI
                     KADADRSEHASDARVRSELAIQADQAAQDCLALAPASAACLYGSAISLGLQARAHPAR
                     ANDFLGRMLDALSKAEAADPTYDEAGPARVRALVLIRAPGWPLGPGDPEAGLAAARRA
                     LALRPQYPPNLLAFAESLAKTGDAHGSHDYYSRARDVARALPPSSERQDWLHEADQAL
                     AHEADQTLSHEADEAVRDD"
     gene            5375..5647
                     /locus_tag="VFB37_12495"
     CDS             5375..5647
                     /locus_tag="VFB37_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23313.1"
                     /translation="MDGHDEVSYDRSVNTQLRDVPRCWRCRVCGFDRYHRVSVMRKNG
                     ARYETSFYACSACSVMFLNDSQFDAASTVSPGIEIPPVVTPLRRKK"
     gene            complement(5669..6925)
                     /locus_tag="VFB37_12500"
     CDS             complement(5669..6925)
                     /locus_tag="VFB37_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZO23314.1"
                     /translation="MREQARKLPVSCAAWVTLAALCASVPARAGGPQDFARIERGRYL
                     TIAADCAACHTDPVSNSAFAGGRSIETPFGMVAAPNITPDRQTGIGTWSDSDFDRALR
                     QGVRPDGKRLYPAMPYLYFTRMSREDVLSIRAYLNTVASVHHAVRANLLPFPFNIRGL
                     MRVWDAWYFTPGEYKPDHAKSPSWNRGAYLVEGPGHCAACHSPKNFLGADRADQRFHG
                     YSLQGWFAPNITADRQRGLGNWSDTDILEYLKKGHNRLAAASGPMAEEVENSSSKMTD
                     ADLAAIATYLKDQPGETADTQRIAETDRYMKVGAAIYSDRCAACHKLDGTGVAYLIPN
                     LAESSSVNSRQPLTALRVILRGTRTVATRSEPTGPAMPSFGWQLNDAEVAAVATYIRN
                     SWGHAASRVTAEDVSKARKSLAARRD"
     gene            complement(6918..>8172)
                     /locus_tag="VFB37_12505"
     CDS             complement(6918..>8172)
                     /locus_tag="VFB37_12505"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZO23315.1"
                     /translation="GGNPFEGPRSRPYPNPAQQQPFGHTLFAKAARELGYKPFPQPSG
                     NMSRAYMNPLGVRMGPCTYCGFCEWFGCGNYSKASPQTTVLPVLLRKPNFQARDNSEV
                     IRINTDRSGKRATGVTFVDTSGNEWEQPAELVILSAYTIFNVQLLLLSGIGKPYDPVA
                     NTGVIGRNFTHQTISDVTSFFDPAKFIFNPFIASGSIGMCIDEFNGDNFDHGPHHFVG
                     GGYMGHVQTNGRPIETTPVPPGTPKWGAKWKQAVRESYQSAIKPGTGVHGSMYSYRDV
                     YLDLDPTYRDRFDRPLVRITMDFHDNEIKQNAFLTDKFAAIFQAMGARQLIKEYRKKP
                     YDITAYQTTHLCGGAVMGTDPKTSALNRYLQSWDVPNLFVMGASAFPQNAGYNPTGTV
                     AALAYWSVAQIRSQYLKSPGPLINA"
BASE COUNT         1493 a         2638 c         2636 g         1405 t
ORIGIN      
        1 gccgctaccg cgccgagctc aaatccaaca ccgaacccgt cgagcatctg cgtgcaatgc
       61 tccgcagctc agcgctgacg ctcctctatg cggcacgcga ccccgaacac aaccacgcac
      121 gcgtgctggc cgagtacctg aagcgcacga gcaagccacg tccgcggcgt tgagctcact
      181 ttccggccgg cttcgcggca cgcgccgctg attgaccggc gcgcctgcgc gcacgcagaa
      241 atttttcaca atcctccagg ctcgggcatc cgcaatggaa gctgctctcg agcagtgacc
      301 tcatctgctg ggtccggtcc atcagcgcat tcagctcttc gagcttgcgg gtcgccaggg
      361 cgcgccagcg ggccgcaggc ggagtctcct tcgagaaccc cgagaggaag atgcgcgtct
      421 cggcgatgct gaagcccgct tcgagcgcca gcttgatgat ctccactcgt gcgaacacac
      481 cttcgtcata acatcgctgg ccagacttgc gcgccggcgc cggtaacagc ccttccttct
      541 cgtagaagcg cagcgccgaa cttgcgactc cgcattcgcg cgccaccgca ccgatcgaca
      601 gatttcgcat tgcacagctc ctgcgccggg gcttgactta aagtgcactt taactcgtag
      661 agtgccggga gtaccagccc gctcatcgat cccgaggact ccacgatgtt gcatctcctc
      721 aaaagaaccc gcgcaatccc gggcatcctc atactcctgg ccgcactggc cgcacgagcc
      781 gaggcgagcg ttcccgacta catcgcggcg gcagtggccg atccggcgcg tccgctgcag
      841 gaccaagcgc gggaccgcga gcgaaaggct gctgaagtcc ttgccttcag cggcatcggg
      901 ccgggcgcgc gggtggcgga cttcatgtca gggggcggct atttcacacg cctcatgagc
      961 cgtattgtcg gagatacggg caaggtgtat gccttcctgc ctgaagagga gctgcggaat
     1021 tgcgtaccgc aggagaccgc cggcacgctg gccctcgagc atgatgcgcg ctacgcgaac
     1081 gtcagcgtgc tgcgctcgcc tgtcaagcag ttccatgcgc ccgagccact cgacctggtg
     1141 tggacctcgc tcaacttcca tgatctgtac gacgccttca tggagcacgc ggatgtaccg
     1201 gccgtcaccc aggcgctgta ccgtgcgctc aagcccgacg gcgtgctgct ggtcatcgat
     1261 catgctgcac agccccattc gggcatacgc gacaccgaga agctgcaccg catcgacccg
     1321 caggtcatca tcagcgcagc actcgcggcg ggcttccgcc tcgatgcccg cagcgatctg
     1381 ctgcgcaatc cggcggacac ccaccagttg ctcgtcttcg atccgcggat ccgcggcagg
     1441 accgatcagg tgatcctgaa attcaggaag cctcggtgag aacccgccgt ttgcctcgac
     1501 tgagaatcca gagcgcaccg ggaagcgcgg tgaaatccgc gagtagcgcg aggatgaagg
     1561 ccgaggagct gacgatcccg aactgacgca tcggaggcag atcggacagc gccagtgcga
     1621 ggaagccgct ggagttgatc aaggtggcga acagaatcgg ccgaccggct acctgcatcg
     1681 cgtggcgcag cgcctcggcg gtgctgccgg aggccgagcc ttcctggaaa tgatagaaga
     1741 aatggatctg gtcgttctcc gtcgcgccga gcacggtgga gccgatgagg atcgtcgcga
     1801 tgttgagtgc aataccaccg agccgcatga tgagaaacac cgccaggatg gcgaacagcg
     1861 agggaatcat cgtcatcagc cgggccgagg gatcgcggaa cacgagcaga aaggccagaa
     1921 aaatgagact cgccgtgatt gcgaaacttt cggtgagcgt aggcagcagc cgctcggtga
     1981 tctcggtccc gagcacaccc ctgcccaccg ccagcgcgtg cgccgagcgc agggcaggct
     2041 cggccgcctg caccgcaagc caggtttgct cgacgtagcg tcgcatcgcg cccgtccgcc
     2101 cgaacagatc cgcgcggccg cgtatgctga tgcgcacgct cgcgaggttg ccgacgtcga
     2161 cgtacgcgcg ggccgccggc tcggtgagca tgatctgctc cagatccgcg gcaagcttcg
     2221 gccaggcatc gggcgcgctg ggcaggcgat ccgaaccgct ttgcacatag cgctcccagc
     2281 gaagcgtcga agtcgggcca tccaccgccg tgattcgcgg atcactctcg agctgatggg
     2341 tgaatcgctc gaggccgcgc agcagttcag ggtccagagc gtgaccgggc gtggtctgta
     2401 cccacagctc gatgacatcg agcccgttcg actgctggaa agtgcgggtg tcctgtgcaa
     2461 cgcgctcgtg tggattgacg taggtcagca cgtcggtttg cagtgacagc ggcgtaagca
     2521 aacccggaat accgaagagc gcgccggcgc cgcagagcat gagcgcgagc gccgctccca
     2581 cgaatggcca tcggtagcgt cgagttgcgg ggaccaatgc ctgcaccagt cccgggaacc
     2641 acttcccggc ggcagctcgc tccgtccgca aaggcgtacg cagcagtgat tgcagcgccg
     2701 gaaacaacgt gaagcaggcg acccacgcca cgatgaggcc gctcgcggtc cacaacccca
     2761 tctcgcgcac gggctggatg tcggagacgg ccagcgcccc gaagccgacg gcggttgcaa
     2821 acatcgaggc ggtgcacggc agaaacttgt tggtcagcgc acgcgcatgg tgctcgagcg
     2881 gattcgcgca gtcgtccggc tccatatacc tcgcatgcag gtagacgagc gtggcggtgg
     2941 tggtcaccat gacagtcaga ggcaccagag tggaaatcac ggtatgcgac cagccgaaca
     3001 ggtcggcgag accaaccgcg atggcaacca cgactgcgag tgtgacaatg attgcgccga
     3061 gtgcacgcca cgagcggtag acgatgaaca ccagcgccat gaggaaaatt ccgaacagcg
     3121 gcatgaatcg cgccgtcgcc gccccggtgc gctgctcgag ccacgcatcg agccacgggg
     3181 agcccacccg gcggatggcc gtgaacggcc cggcaccgga cccgagaggg cgcagcacag
     3241 agtcgatcgc tgcaagcgta cgatcccgct cggccggtgt cgccacgcgc aactcgagcg
     3301 cgacacccag ataatgctca cccaggagcc cggcccgtcg aaagagcgtc gtccctgtcg
     3361 cgaatgcgcg cacccgcgcg atctcggccg catcgagctc acgttcgccg ttggcccgcc
     3421 ggtacagcgt gagcaggctg tgtgcctcga cgtgcgggat gcgaccgagc tcgctctcga
     3481 tctgctgcgc agcctgcaac gatctcaggc tgagcgggtc cggcgcctcg agcatcagca
     3541 gtgcctgatc gccttccgga tacaagcgat cgaaatccgc cgtggctcgg gcaaccggat
     3601 ctgcggctac gacgagacga tcgatcgcgg gatcatcgcg cacctgtagc gcaccgtaga
     3661 tgcccacggc aaacagcgct agaaagcagc ccgcgatcca gactcgagcc tggatcacgc
     3721 gggtgaagaa ttggaccatc ttgggcgaga cgggcacact cattggtaga agtgcgctcg
     3781 ttctcgggtc cgatccgcag cgcgcaggga tgatagccta aacacatgag cagcttgctg
     3841 acggcggcca ttgcgacgct ggtgttctgc gcgcccggtt atcccggcgc ggccggtgat
     3901 gcacagcctt tcatcgatca attcgtgagt gcggccacag cgaatgccgg ctggcccaag
     3961 ggcagccttg cggccgtcta cgacccgagc gaagcaggtg gcctcacgaa gctcacaagc
     4021 cccgatgcgg tgctcgcgtt catcccttat cccttttttg tcgagcatgc ggctcagctc
     4081 catctcacgc cgcttgcgca ggcggacgtg acgggcctgg gcaccaccga acgttggacg
     4141 ctggttggta agtcgggtct gcccgaccgg ccgcaagcgc tgaacggcta cacgatcctc
     4201 agcgtagccg gctattcgcc ggtattcgtg agacagtccg ccctcggcgc gtggacgctg
     4261 ccgcagggcg ttcgggtgga atcgacggga cagatcctga gcgcgctgcg tcgggtcgca
     4321 tcgggcgaga cggtggtagc gctcctcgat cagactcagg ccgctgcgat acccacgcta
     4381 cccttcgctg cgcaactcac ccccatgctg cagtcggcgc cggttccggt cgcccttctc
     4441 gcgatcgtgg attcgcgtct caccccggcg cgcgcgcatg cgctgcaagg cgctctattg
     4501 aagatgggcc acgccggctc cgatgccgac gtgctgggcg ctctgcattt gcagaggttt
     4561 gtcgcgcccc agctgcctgc ttcccggatc ggtccgtgag tgggcgtcga gcggccgccg
     4621 ccgccctggc actcgcaatt gcggcctgta caaccacccc tcgacccgct gatcgggctc
     4681 cagagcaccc gcagaatgcc tcggtgatcg agctgtcggg cgccatcaag gcagatgcgg
     4741 accgtagtga gcatgcatcc gacgcacgcg tgcgcagcga gctcgcaatc caggcggatc
     4801 aggcggcaca ggattgcctg gcgctcgcgc ctgcgtcggc cgcctgtctg tacggcagcg
     4861 cgatttccct tggacttcag gcccgggctc accccgcgcg tgcgaacgat ttcctgggca
     4921 ggatgctcga tgcgctctca aaggccgaag cggccgatcc tacctacgat gaggccggtc
     4981 cagctcgtgt ccgcgcgctc gtgttgattc gtgcaccagg ttggccgctc ggaccgggag
     5041 atccagaagc gggtctggcc gcggcgcggc gggcgctcgc gcttcgcccg caatatcccc
     5101 ccaacctgct cgccttcgca gagtctctgg ctaaaaccgg agatgcgcat ggctcccacg
     5161 actactactc gcgcgcccgc gatgtggccc gagcgcttcc gccctccagc gagcgtcagg
     5221 actggttgca cgaagctgat caggctctgg cgcatgaagc tgatcagacc ctgtcgcacg
     5281 aagctgatga ggctgtgcgc gacgactgag ctcggcagct tcagccccgc ccccgccggg
     5341 gcgccataca gtgaggcacc gaactcggta ctgaatggac gggcacgatg aggtttccta
     5401 cgatcgcagc gtgaacacgc agctacggga tgtgccgcgg tgctggcgtt gccgcgtatg
     5461 cggtttcgac cgctatcatc gcgtcagtgt catgcgcaag aacggcgcga gatatgagac
     5521 ctcgttctac gcctgcagtg cgtgcagtgt catgttcctg aacgacagcc agttcgatgc
     5581 tgcaagcacc gtcagtcccg gcatcgagat cccaccggtc gtaacgcccc tgcgccgcaa
     5641 gaagtgagcg ccgcgccgcc cgcgttgctc agtccctgcg cgcggccaga ctcttgcggg
     5701 ccttgctcac atcttcggcc gtgacgcggg aagcagcgtg accccagctg ttgcggatgt
     5761 aggtggcgac cgccgctacc tctgcatcgt tcagctgcca gccgaatgat ggcatggcag
     5821 ggcccgtcgg ctccgagcgg gttgccacgg tgcgcgtgcc acgcagaatc actcgaagag
     5881 cggtcaacgg ctggcgcgag ttgacggaag aggactcggc caagttcgga atgagatagg
     5941 cgacaccggt gccgtcgagc ttgtgacacg cagcgcagcg atcggagtag atggccgctc
     6001 ccaccttcat gtaacgatcg gtctcggcga ttcgctgggt gtccgcggtc tcgccgggct
     6061 gatccttcag gtaggtcgca atggccgcca ggtccgcatc ggtcatcttc gagctggaat
     6121 tctccacctc ctcggccatc ggtccggagg cggccgccag gcggttgtgg cccttcttca
     6181 ggtactccag gatgtcggta tcggaccagt tacccaggcc gcgctggcga tccgcggtga
     6241 tgttgggagc aaaccagcct tgcagcgagt agccgtggaa gcgctgatcg gcacgatcgg
     6301 cgccgaggaa gttcttcggc gaatggcaag cggcgcaatg ccccggccct tccacgaggt
     6361 aggcgccgcg attccagctc ggtgatttgg cgtgatcggg cttatattcc ccgggagtga
     6421 agtaccaggc atcccacacc cgcatcaggc cgcggatgtt gaacggaaac ggcagcagat
     6481 ttgcccgcac cgcgtggtgc acggaagcca cggtgttgag gtaagcacga atggagagca
     6541 catcctcccg gctcatgcgc gtgaagtaga gatatggcat ggctgggtaa agccgcttcc
     6601 cgtcaggcct gactccctgg cgcaacgcgc gatcgaagtc cgagtcgctc caagtgccga
     6661 tgcctgtctg gcgatcgggc gtaatgttgg gggcggccac catgccgaag ggggtctcga
     6721 tggaccgtcc gccggcgaag gcgctgttgc tgaccggatc ggtatggcag gcggcgcaat
     6781 cggctgcgat ggtcaggtag cggcctcgct cgatgcgtgc gaaatcctgc ggcccgccgg
     6841 cgcgggccgg cacgctcgcg cacaatgcgg cgagcgttac ccacgctgca cagctcacgg
     6901 ggagcttgcg cgcctgctca cgcattgatc agtggcccag gactcttgag atactgcgag
     6961 cggatttgtg ccacggacca gtaagccaga gccgcaacgg tgccggtggg gttgtagccc
     7021 gcattctgcg gaaaggcgct cgcgcccatg acgaacagat tcggcacgtc ccagctctgc
     7081 agatagcggt tgagcgcgct cgtcttcgga tcggtgccca tgaccgcgcc tccgcacaga
     7141 tgcgtcgtct gatatgccgt gatgtcgtaa ggcttcttgc ggtactcctt gatgagctgc
     7201 cgggcgccca tcgcctgaaa gatcgcggcg aacttgtcgg tcagaaaggc attctgcttg
     7261 atctcgttat catggaaatc catcgtgatg cgcacgagcg gccggtcgaa gcgatcgcga
     7321 taggtcggat ccagatcgag atagacatcg cggtagctgt acatgctgcc gtgcaccccg
     7381 gtgccgggct tgatcgcact ctggtagctc tcgcggacag cctgcttcca tttcgcgccc
     7441 cacttgggcg tgccgggcgg cacgggcgtc gtctcgatgg gccgcccgtt cgtttgcacg
     7501 tgacccatgt agccgccccc gacgaagtgg tgcggtccat gatcgaagtt gtcgccattg
     7561 aactcatcga tgcacatgcc gatcgagccc gaagcgatga atggattgaa gatgaacttg
     7621 gcgggatcga agaagctcgt gacatccgag atcgtctggt gagtgaagtt gcgaccgatg
     7681 acgcccgtgt tggcgaccgg gtcgtacggc ttgccgattc cggacaaaag cagcagctgt
     7741 acgttgaaga tcgtgtaggc cgagagaatg accagctctg cgggctgctc ccactcattg
     7801 cctgatgtgt caacgaacgt gacaccggtt gcacgctttc ccgagcggtc agtgttgatc
     7861 cggatgactt ccgaattgtc ccgcgcctga aaattcggct tgcgcagcag caccggaagc
     7921 accgtggtct gtggactggc cttcgaatag ttgccgcacc cgaaccactc gcagaagccg
     7981 caataggtgc agggacccat tcgcactccg agcggattca tatacgcacg ggacatgttg
     8041 ccggagggct gcgggaaggg cttgtagccc agctcgcgtg cagccttggc gaacaaggtg
     8101 tggccgaatg gctgctgctg cgcggggttg ggataaggtc gcgatcgcgg tccctcgaag
     8161 ggattgccgc cc
//
LOCUS       DASRHN010000318        18724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_442229, whole genome shotgun sequence.
ACCESSION   DASRHN010000318 DASRHN010000000
VERSION     DASRHN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18724
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442229"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2304)
                     /locus_tag="VFB37_12510"
     CDS             complement(<1..2304)
                     /locus_tag="VFB37_12510"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZO23316.1"
                     /translation="MTRQDASRYWAFISYSHADSRWADWLHRAFEQYRIPRRLMGLPT
                     RAGAVPSRLAPVFLDRLDLAAGVDLDQSIAEALSSSRALVVICSPKARDSARVAAEID
                     TYRATGAGPILPLIVAGRPDATLRGGASNEECFPAALKAALAARPVASPLAADVRAGK
                     GGRQEALLKLIAGILDLNLDQLRQRDLQRRRVRWLTSMVVFACAMLITSGLALYAWQQ
                     RNSALAAQSRLLAQAAYDRLLEGDVGDAQRIILEVLTNPSFRSEYLGAAAVNVFQEVR
                     GRDAQVALLYGQASRIANASYSPDGSRILTVGVDGTAWIWDATTFVPLHVLAGHKARL
                     FSGEFSPNGRLIVTGSRDRTVRIWDAHTGGQLRILSGHTDNVRSVAFSSDGTRVVSAS
                     DDGTARIWDATTGSTIAILVGHGDGLTSATFSPDDSQVLTASADRTARIWDAHTGTLR
                     RVLSGHTDLLNAAIYSPDGRIIATASQDHTARLWNSQTGALIAVLSGHTDSVRAVAFA
                     PDGEQLVTTAQDGTVRLWEAHTARPLAVLSGHDSWVISAHYSPDGSKIATTGADHTAR
                     LWDAHRGKLLATFRGHHDHVASARFSPDGRSLVTASDDFTARVWNVLPGDQVASFTVP
                     AGRVNSVDYSSDGSRILLATAGTEASVWDPQKGKPLVRFNGHTQMVNTAVFSPDGKTA
                     LTASRDATARIWDAGTGALLRVLRAHTLSVEGAAYSHRGDRIVTASMDLTARVWDAHS
                     GAPLLILKGHTDRLSSAQFSPDDEHIVT"
     gene            2377..3216
                     /locus_tag="VFB37_12515"
     CDS             2377..3216
                     /locus_tag="VFB37_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZO23317.1"
                     /translation="MADPQPHRYKHPHPAVAVDLVIFTLLNGQLQLLLIERGIEPYRG
                     AWALPGGFMRIDEDLVAAAQRELVEETGIGEAYLEQVGAFGQPKRDPRERVVSVAYFA
                     ILPAHKVALAAGSDAKAVAWHPYSELPELAFDHASIVAAAKAKLAEKVNRTTVALEFL
                     PPEFTLTELQQVFETIRGETLDKRNFRKWAGTLSYIKATGRLRRGGQHRPAALFKPAV
                     KGAMPIPTASLEWTTVATEKSSASEAAAKAQYRRGFEDAVSAINKAFADSSKGVLRAL
                     ASR"
     gene            3701..5011
                     /locus_tag="VFB37_12520"
     CDS             3701..5011
                     /locus_tag="VFB37_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZO23318.1"
                     /translation="MRKRLCARFILGLAAATLVSGAATAAETEFSVLHTFDVANATTG
                     DSPGGSQPDTRPMLGPDNTVYGMTYTGGANGTGVIYKYGLSTHKYSVIHTFGALDANM
                     DNEDGAYPGVALTRGPDDVFYGMASFGGTNGNGTVFSVTPEGKFKVLHEFSALDANLH
                     NEDGANPLRTIVVASDGNLYGTTRTGGENGLGVAWVIGRSGGFIVVHQFTSTEGHAAS
                     LLQARDGYFYGCAVFPNPTLGSGILYRMDPSGHHFEVLYRFTPINKNGANEDGANCYE
                     PLVERHPGVFYGTTRSGGNGNGVVFRYSLAQPNRVAVIHNFSVCETSGATCINTDGAS
                     PESRLTLRGDSALYGTASNGGTGGSGVVFRVRPDEAFEVLHTFSAVNATTGANKDGAL
                     PDFGVIFAGDDDLIGMADAGGKGDASYGFGNGTLYRLSIERRER"
     gene            complement(5114..6949)
                     /gene="glmS"
                     /locus_tag="VFB37_12525"
     CDS             complement(5114..6949)
                     /gene="glmS"
                     /locus_tag="VFB37_12525"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZO23319.1"
                     /translation="MCGIVGAVAERNIVPILMEGLRRLEYRGYDSAGIAVLNGTQHLK
                     RLRTVGKVRMLDEALEQEPTDGKVGIAHTRWATHGIPSERNAHPHISRDGLAIVHNGI
                     IENHDELREELVRSGYQFSSETDTEVIAHRIHRHLQTVKDLFKAVRATVAELEGAYAL
                     AVVSERDPERIILAREGCPVVIGLGVEENFVASDVAALLPVTRRFIFLEEGDVAEIGR
                     RSVRILDKEGNRVERPVRESDLSADAAEKGPYRHFMLKEIHEQPRAIANTLQERVAHG
                     RLLEAAFGPAATEVFKRTANVHIVACGTSYHAAVVARYFMEQICKLPCTVDIASEYRY
                     RNPLVPPNSLFVTISQSGETADTLAALRLAKESGYLSSLSICNVPESSLVRESELVML
                     TRAGPEIGVASTKAFTTQLAALGMLVIALAKHNGADAERERGLVTRLIELPALIEKTL
                     ELNPVIHKLAERFADKHHALFLGRGALYPIALEGALKLKEISYIHAEGYPAGELKHGP
                     LALVDADMPVITVAPNNDLLEKLKSNLMEVRARGGELIVFADPEAQIQPSDGVTVIEM
                     PKHVTYFQAPAVYTVPLQLLSYHVAILRGTDVDQPRNLAKSVTVE"
     gene            7175..7972
                     /locus_tag="VFB37_12530"
     CDS             7175..7972
                     /locus_tag="VFB37_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZO23320.1"
                     /translation="MLIEKSTRPPGTPLSVRLLLTSLMLCALSSVGLADQSKPDAAAT
                     IRKTLEARYPGTHVLDVQPSAMAGVYEVFLGDEIVYTDASADYILMGPMVDTQTRKDL
                     TEARMNEHGRIDFDLLPLARAIKVVKGNGSRRFAVFSDPDCPFCQQLEKSLLPISNVT
                     MYVFLYPIAALHPQAPAKAHAIWCAKDRAQAWNEWMHEKKLPATGQCAGDPVADLQKL
                     GDTLRINSTPTLFFANGRRVAGAISTAEIEQFLSASDIQKPVAPAGK"
     gene            complement(7999..9381)
                     /gene="glmU"
                     /locus_tag="VFB37_12535"
     CDS             complement(7999..9381)
                     /gene="glmU"
                     /locus_tag="VFB37_12535"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZO23321.1"
                     /translation="MAKKDSLPLHAVILAAGQGKRMHSDLPKVLQPLAGRPLLKHVID
                     TARSLEPKALHVVYGHGGERVREELQEPGISWVLQAERLGTGHAVLQAMPQVPDEALV
                     LVLYGDVPLIGRSTLTELVSIAGPRKAALLTVILEEPTGYGRVVRNSRGQVRRIVEQK
                     DASKKELRLTECNTGVLAAPGRSLRKWLKAVTNDNAQGEYYLTDVIAMAVKEGLAVQP
                     LVAERVTEVLGVNDKAQLATLETAYRRGITRQLMLAGLTIADPERVDVRGSLTHGRDA
                     VLDVNVIIEGHVKLGDRVRIGPNTVIRNSEIGDDTEVFGHCVIDSTVIGPNCNIGPFA
                     RFRPTSTLAGGVHIGNFVEVKNSRMGAESKANHLAYVGDAQLGERVNIGAGTIIANYD
                     GANKHRTTIENDVHTGSNSVLVAPITIGAGATVGAGSTVVKQVPPRKLTVARARQITI
                     ENWQRPSKPK"
     gene            complement(9414..10691)
                     /locus_tag="VFB37_12540"
     CDS             complement(9414..10691)
                     /locus_tag="VFB37_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HZO23322.1"
                     /translation="MRSFDQLAALVLINLLVWAALDTLHAEAGSQLMLDGLYTWAFYL
                     LVGLFACGLVARAQSRSADTRAVLIPALSASPYVLIAFWLLSDLHQVSSHPLGALVAG
                     TLYLLLLGVRVLQSAFVTPSRTAVLLAAVFIAAAPWALQSLNLDTRLWLTADSQDEQS
                     DDTSAEALLYDQPGRIAGSVEHMAARQPGEPNVFFVGFAGDGEQGIFKREALYAEQVF
                     ADHFGSGDHSIELINDDNDRDSYPIASVSALGQALRLVASHMDTEQDVLVLLLTSHGS
                     RDGLAIVNGSLPLQQLAPADLRQALDDSGIKWRVVIVSACYAGVFLDPLKSDDTLVMT
                     AADSNHSSFGCDDARDLTWFGEAFLQDSVPGSRTLEDAFRKATGIIQRREAAEHEVHS
                     NPQIWAGTAIRQQLAALEGGAGGRSRHATIIAR"
     gene            complement(10796..11230)
                     /locus_tag="VFB37_12545"
     CDS             complement(10796..11230)
                     /locus_tag="VFB37_12545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZO23323.1"
                     /translation="MAETQQSTVDLHIVSAEGELFAGPAAMVLVSGCMGDLGILPRHA
                     PLLSLLKAGELRVKTPDGQEHSYFVGGGVLEVQPHKITVLADTAARARDLDEAAALEA
                     KQRAEEALQDKTGHIDLAHAQAELARAAAQLKLIERLRKLRS"
     gene            complement(11240..12670)
                     /gene="atpD"
                     /locus_tag="VFB37_12550"
     CDS             complement(11240..12670)
                     /gene="atpD"
                     /locus_tag="VFB37_12550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZO23324.1"
                     /translation="MTHAVEGKITQIIGAVIDVAFPRESIPHVYEALKLKDADLTLEV
                     QQQLGDGIVRTIAMGSSEGLKRGLPVLATGAPISVPVGKGTLGRIMDVLGRPQDNRGE
                     VQAKEHLPIHRPPPSFEDQAGGQDLLVTGIKVIDLLVPFAKGGKVGLFGGAGVGKTVN
                     MLELINNIAKQYSGLSVFAGVGERTREGNDFYHEMIESGVIDKDNLSNSKVAMVYGQM
                     NEPPGNRLRVALTGLTMAEYFRDEKREDGGRGRDVLFFVDNIYRYTLAGTEVSALLGR
                     MPSAVGYQPTLAEEMGVLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDAT
                     VVLSRQIASLGIYPAVDPLDSTSRQLDPLVVGEEHYQTARGVQAVLQRYKELQDIIAI
                     LGMDELSEDDKLTVYRARKVQRFLSQPFNVAKVFTGQDGKIVPLKETIRGFKGIIGGE
                     FDSLPEQAFYMVGGIEEAVAKAKSLQ"
     gene            complement(12708..13568)
                     /gene="atpG"
                     /locus_tag="VFB37_12555"
     CDS             complement(12708..13568)
                     /gene="atpG"
                     /locus_tag="VFB37_12555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZO23325.1"
                     /translation="MPGTKEIRTKIASVKSTQKITRAMQMVATSKMRRAQERMKQARP
                     YAQEMRNVIGHLTDANPDFKHPFLVAREPKNIGIIIVSTDRGLAGGLNANVFRQTIHL
                     MREWQEKGAQVSLCIIGTKGLNFFRRLRVPILANTSGLGDQPHIKDLIGTVKVMLDAY
                     REGKVDRVFLVNAHFLNTMTQKPTVEQLVPVEGRDTENLPEHWDYIYEPDARDILDGL
                     LMRYVESQVYRGVVENVASEMAARMVAMKAATDNAGKLISELQLVYNKARQAAITKEL
                     SEIVGGAAAV"
     gene            complement(13584..15125)
                     /gene="atpA"
                     /locus_tag="VFB37_12560"
     CDS             complement(13584..15125)
                     /gene="atpA"
                     /locus_tag="VFB37_12560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZO23326.1"
                     /translation="MAIKATEISDLIKQRIENFKGVTEARNVGTIVSVTDGITRIHGL
                     ADARYGEMLEFPGNVFGLALNLEQDSVGAVVLGEYKDLVEGATVKTTGRILEVPVGPE
                     LLGRVVDALGEPLDGKGPIKAARTAPIERVAPGVIYRKSVSQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAVDTIINQKDSGIRCIYVAIGQKQSTVANVVRKLEEQGAM
                     AHTIVVAASASSPAALQYISAYSGVTMGEYFMDNGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERSARVNEEYVEMVSKGAVKGKTGSLTGLPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGIRPAMNAGISVSRVGGAAQTDIIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRRQLERGQRTTEVMKQKQYTPMSVAQMALSIYA
                     VNNGYMDKIELKKVVDFEAGLQAFADTNYRQMLDSVNANPKLTKENEAALKKCIEDFV
                     STGTY"
     gene            complement(15148..15684)
                     /locus_tag="VFB37_12565"
     CDS             complement(15148..15684)
                     /locus_tag="VFB37_12565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZO23327.1"
                     /translation="MADRTTIARPYAKAAFEESQRAKTLGRWSETLRAAATVVQDPRV
                     KRLLGNPAVRKADLAAFVADLAGPQLDETGRNFVRTLADNRRLAYLPEISALFDQLKN
                     EAEGVVDVTVTSAAPLVNGQLEALSTALQRRLKRQIRVQCHVDPALIGGAVLRAGDLV
                     IDGSLRARLERIAYELTA"
     gene            complement(15701..16171)
                     /locus_tag="VFB37_12570"
     CDS             complement(15701..16171)
                     /locus_tag="VFB37_12570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZO23328.1"
                     /translation="MNINLTLIIQMIVFALFIWFTMRFVWPMILGAMEERSRKIAQGL
                     AAAEKGQQELAQARERSETIIREARERANQIIDQAQHRANDLVEHAKGAATVEGQRLV
                     EAAHQRIELDTTRARESLRQEVAEIAVSAASKLLEREIDPKAHADLLGKLAAQI"
     gene            complement(16221..16496)
                     /gene="atpE"
                     /locus_tag="VFB37_12575"
     CDS             complement(16221..16496)
                     /gene="atpE"
                     /locus_tag="VFB37_12575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZO23329.1"
                     /translation="MENIAHIQAMTVLACGIIFGSGAFGTAIGFGILGGRFLEGMARQ
                     PELAPALLARMFIVAALLDAVAFIGIAFALFFMFANPFLTAVQAAAH"
     gene            complement(16547..17413)
                     /gene="atpB"
                     /locus_tag="VFB37_12580"
     CDS             complement(16547..17413)
                     /gene="atpB"
                     /locus_tag="VFB37_12580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZO23330.1"
                     /translation="MFLARAAAEAQSPTEYITHHITFLTNVAPRGILDLSVVNLDSVF
                     FSILLAVVFGGAFYAAARKATTGVPGKFQNFVEWIVEFVDTQVRTTFQGQSKLIAPLS
                     LTIFGWVLCFNLMDVIPIDLLPSAAHGAGLGHLKIVPSTDLNIVFGLSITVFLLIIIY
                     SIRMKGFGGFVGSLTLHPFSSKNVLLQALLVPVNFVMEFPSFIARPVSLALRLYGNMY
                     AGEMVFALLALITLSRGLESLTSPGSWGWGLLQAALGVLWSLFDTFIALLQAFIFMML
                     TIVYLSQASEHH"
     gene            complement(17438..17857)
                     /locus_tag="VFB37_12585"
     CDS             complement(17438..17857)
                     /locus_tag="VFB37_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZO23331.1"
                     /translation="MVAIELADARRLAFGVVLGQVVATLIVACLCWLLAGWNSAVSAL
                     LGGGIAAAASLAMTVLAFGRWASGSAERLLAAFYVGELAKLVVVIALFTLVLTTLRPA
                     PAAMLAAYGATFLVYWIVLAGFVPGLGGLRRLRGLRG"
     gene            complement(18176..>18724)
                     /locus_tag="VFB37_12590"
     CDS             complement(18176..>18724)
                     /locus_tag="VFB37_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZO23332.1"
                     /translation="IRRIPDEAAIAVALIENIQRENLNPLEEARALDRLITEFTLTHQ
                     QAADAVGRSRAGVTNLLRLLELAPEVSDRVERREIEMGHARALLALTNRQRQVEVSTL
                     IVTKGLSVRDTEALVRRMQQPAVAPETAPPGDPNVHRLEQELADKLGARVAIQHTTAG
                     KGKLVVSYNTLDELDGILAHIH"
BASE COUNT         3455 a         5971 c         5858 g         3440 t
ORIGIN      
        1 tgtgacgatg tgctcgtcgt ccggtgagaa ttgtgcagag ctgagccggt ctgtgtgacc
       61 cttcaagatc agtagtggcg cgccactgtg cgcatcccac accctggccg tcagatccat
      121 gctggcagtg acgatgcgat caccacgatg cgaatacgcc gcgccctcaa cagaaagggt
      181 gtgcgccctc aacactctga gaagtgcccc ggtgccggcg tcccatatgc gcgcggtcgc
      241 atcccgtgaa gcggttaaag cagtcttccc gtccggggag aagaccgccg tattgaccat
      301 ctgggtgtgc ccgttgaatc tcaccaaggg cttgcccttc tgcggatccc acacggaggc
      361 ttcagtgcct gcggtcgcca gcaggatgcg tgagccatct gaggaataat caacagagtt
      421 cacacggcct gcggggaccg tgaaggatgc gacctgatca ccaggcagca cgttccagac
      481 ccgagctgtg aaatcatccg acgccgttac caacgatcgt ccgtcagggg agaagcgagc
      541 gctggcgacg tgatcgtgat ggcctctgaa ggttgcgagc agcttgcctc gatgtgcgtc
      601 ccatagcctt gccgtatggt ccgcgccggt ggtcgcgatc ttcgagccat ccgggctgta
      661 atgcgcgctg atgacccaac tgtcgtgccc cgagagcacg gcgagcggcc tggcagtgtg
      721 cgcttcccag agccgaaccg taccatcctg cgcggtcgtg acgagttgct ctccgtcagg
      781 tgcgaaggcc accgcccgga ctgaatccgt atgacctgac agcacggcaa tcagagctcc
      841 ggtctgcgag ttccacagcc gcgcggtgtg atcctgtgaa gccgtggcga tgatgcgccc
      901 atctggagaa tagatcgccg cgttgagcag atccgtgtgg ccactcagca cccgtcgcag
      961 agtcccggtg tgcgcatccc atatgcgcgc tgttcggtcg gcggaagccg tcaggacctg
     1021 actgtcatcg ggcgagaagg tcgccgaagt gaggccgtcc ccatgaccga caagaatggc
     1081 gatggtgctg cccgtcgtcg cgtcccagat acgtgcagtc ccgtcatcgg aggcgcttac
     1141 gacccgcgtc ccatcgctcg agaaagcgac actgcgcacg ttgtcggtat gccctgacaa
     1201 tatccgcaac tggcctccgg tgtgcgcatc ccagatacgc accgtccggt cgcgggaacc
     1261 cgtgacgatg agccgaccgt tgggagaaaa ttcgccactg aagagccggg ccttgtgtcc
     1321 ggccaggaca tgcaagggga cgaaggtagt ggcgtcccaa atccaggcgg tgccgtccac
     1381 gccgacggtc agtatccggc tgccatcggg tgaatagctg gcgttggcga tccggctcgc
     1441 ctgaccgtaa agaagcgcga cctgtgcatc gcggccgcga acctcctgga atacattgac
     1501 ggcggcggct cccaagtact cggatcggaa gctcggattg gtcagaactt cgagaatgat
     1561 ccgctgcgca tcgccgacgt cgccctccag caggcgatcg taggcagcct gcgccagcaa
     1621 ccgtgattgt gccgcgaggg ccgagttgcg ctgttgccac gcatacaatg cgagtccaga
     1681 cgtgatgagc atagcgcagg caaacacgac catcgaggtc agccagcgca cacgccgccg
     1741 ttgcagatcg cgctgccgta gttgatccag gttcaggtcc aggatcccgg ctatcagctt
     1801 cagaagggct tcctgacggc ctcccttgcc ggcgcggaca tccgcggcca gcggactggc
     1861 gacaggcctc gctgcgagtg ccgccttcaa agcggccggg aagcattctt cattggatgc
     1921 accgccgcgg agggtggcgt ccggccgacc ggcgacgatc agcggcaata tcgggccggc
     1981 gcccgtcgct cgatacgtgt cgatttcggc tgctacgcgg gcagagtccc tggctttcgg
     2041 cgagcagatc accacaaggg cgcgggagct actcagcgcc tcggcgatac tctggtcgag
     2101 gtcgactccg gccgccaggt cgagccgatc gagaaagacc ggcgctagcc ggctcgggac
     2161 cgcgcccgct cgcgtcggca gtcccatgag acgtcgtggt attcgatatt gctcgaaagc
     2221 tctgtgcagc cagtcagccc agcgactgtc tgcatggctg tagctgatga acgcccagta
     2281 gcgggatgcg tcctgccgag tcacgggtct tgcgccttac ttcgtgtata atcgatctaa
     2341 cgatagtgtc cctttcagaa ccgccaggac gaatccgtgg cagaccccca accgcatcgc
     2401 tacaagcacc cacacccggc ggtcgctgtc gacctcgtca tctttacact gctgaacggg
     2461 cagttgcagc tcctgttgat cgagcgcggc atcgagccgt atcgcggcgc ctgggctttg
     2521 cccggcggat tcatgcgaat cgatgaagac ctggtggcgg ccgcccagcg ggagctcgtg
     2581 gaggaaaccg gcatcggtga ggcttatctg gagcaggttg gggcgttcgg ccagcccaaa
     2641 cgcgatccgc gcgaacgtgt agtcagtgtg gcgtactttg cgatccttcc agcacataag
     2701 gttgcgctcg cggctggcag tgatgccaag gcggttgcct ggcatccgta ctctgagctg
     2761 ccggagttgg cgttcgacca cgcgagcatc gttgccgccg caaaagccaa gctcgccgag
     2821 aaggtgaatc gcacaaccgt cgcgctcgag tttctgcccc ccgagttcac gctgacggag
     2881 ctgcagcagg tgtttgaaac catccgcggc gagactctcg ataagcgcaa tttccgcaag
     2941 tgggctggca cgttgtcgta catcaaagcc actgggcgct tgcgtcgtgg cgggcagcac
     3001 cggccggcgg ccctcttcaa acccgccgtc aagggggcga tgccgatccc gacggcgagc
     3061 ctcgaatgga cgacggtggc aactgaaaag tcgagcgcga gcgaggcagc ggcgaaggct
     3121 cagtaccgcc gcggcttcga ggatgccgta agcgccatca acaaggcctt cgccgattcc
     3181 tcaaagggcg tactgcgagc gctggcctcc cgctaggccg atagctcgat agggcgcgag
     3241 cccgcctcct ttcgtcttcg ctccgtcgca gcttgcggct ctcccgcctc cgcagcgtca
     3301 tagcctctga ctcgatagtg tcaataacgc tattacgaaa gtgttgacaa ttgtatcccg
     3361 gtcttctagg cttcttttag atccagcgaa acactaacga aagatcattt ttacccctat
     3421 caaggagcgg gccccaggga ggctctcaga tgcacgcagt gctgcacggc tttggccgcg
     3481 gcgcgactgg ctcacccgaa tcaccctctg ccgggtggat ttcgcccggc cgcacggccg
     3541 gcagggtcgg ctctgcatcg ctgcacacga attggtgctg gcgctcagat gggcggcagc
     3601 agccacgttg gccatcgcgc cacgatcgga ggcgaaccgc gacccgcggt ccgcaccata
     3661 agacgttgtt ccaacagcca tgactcgatt ggagacatca atgagaaagc gattgtgcgc
     3721 tcgatttatc ctgggacttg ccgcggcgac gctggtcagc ggcgcggcaa cggcggccga
     3781 gaccgaattc tcggtgttac atacgttcga tgttgcgaac gccacgacgg gtgacagccc
     3841 cggcggctcg cagcccgata cccggcccat gctggggccg gacaacacgg tctatggcat
     3901 gacctatacc gggggtgcga acgggactgg tgtgatatac aaatacgggc tgtcgaccca
     3961 caagtactcg gtgatccaca ccttcggagc tctcgacgcc aacatggaca acgaggatgg
     4021 tgcgtatccg ggagttgccc tcacgcgtgg gccagacgat gtcttctacg gcatggcctc
     4081 gttcggcggc acgaatggca acgggactgt cttctcggta acccccgagg ggaagttcaa
     4141 ggtgttacac gagttctccg ctctggatgc caatctgcac aatgaggacg gcgccaatcc
     4201 tctgcgtacc atcgtggtcg cctcggatgg caacttgtac gggacgaccc ggaccggggg
     4261 tgaaaatggc ctgggggtcg cctgggtcat tggccgatcg ggcggcttta tcgtggtgca
     4321 tcagttcact tccacggagg gccacgccgc ctcgttgttg caagcccgtg atggctactt
     4381 ctacggttgc gcggtgtttc caaatcccac gctgggctcc ggcatcctgt acaggatgga
     4441 cccttccggg catcacttcg aggtcctgta tcgcttcacg ccaatcaaca agaacggcgc
     4501 gaatgaggac ggggctaact gctacgagcc cctcgtggaa cgacatccgg gagttttcta
     4561 cgggactacc cggtccggcg gcaatggcaa cggcgtcgtc tttcgctact cgctggctca
     4621 accaaatcgt gtcgcggtga tccataactt cagtgtttgt gagacctccg gcgccacttg
     4681 catcaacacc gatggcgcca gtccggaatc acgcctcacc ctgcgcggcg acagtgccct
     4741 ctatgggacg gcctcgaatg gtgggaccgg cggttcgggc gtggtatttc gcgtacgtcc
     4801 ggatgaggcc tttgaagtgc ttcatacctt cagtgcggtg aatgcaacga cgggagccaa
     4861 caaggacggg gcgctgcccg acttcggcgt catattcgcc ggcgatgacg acctgatcgg
     4921 catggccgat gcgggaggca agggcgatgc gagctacggc ttcggaaatg gaacgctgta
     4981 tcgtctgagc atcgagcgac gcgagcgtta gaagaaggga gcgcgcctct tcctgcgaag
     5041 agtcaggttc ccggcggtgg ccgtggctgc ccacgggcag tcatggccac cgttccgtct
     5101 gtgtcgagcg cagctattcc accgtcacgc tcttcgccag attgcgcggc tgatccacgt
     5161 ccgtgccgcg aagaatcgcc acgtgatacg agagcagttg caagggaacg gtgtacaccg
     5221 ccggtgcctg gaagtacgtg acgtgcttgg gcatctcgat gaccgtcacg ccatcgctcg
     5281 gctggatctg tgcctccgga tcggcaaaca cgatgagctc cccaccgcgg gcacggacct
     5341 ccatgaggtt cgacttcagc ttctcgagca gatcgttgtt cggagccacc gtgatcacgg
     5401 gcatgtccgc atcgacgagt gccagcggac catgcttgag ctcgccggcg ggataaccct
     5461 cggcgtggat gtaggagatc tccttcagct tcagagcgcc ttcgagcgcg atcggataga
     5521 gcgcgccccg tcccaaaaac agcgcgtgat gcttgtcggc gaagcgctcg gccagcttgt
     5581 ggatgaccgg attgagctcg agggtcttct cgatcagcgc cggcagctcg atcagccgcg
     5641 tgaccaggcc tcgctcgcgc tcggcatccg cgccattgtg tttggccagg gcgatgacca
     5701 gcattccgag cgccgcaagc tgagtcgtga aggccttggt cgaggcgact ccgatctcgg
     5761 gaccggcacg cgtcaacatc acgagctccg actcgcgcac cagcgagctc tccgggacgt
     5821 tgcagatcga aagcgaggac aggtagcccg attccttcgc gagccgcagc gcagcgagcg
     5881 tgtcggcggt ctcgccggac tgggagatcg tgacaaagag ggagttgggc ggtacgagcg
     5941 gatttcgata tctatactcg ctcgcgatat ccaccgtgca aggcagcttg cagatctgct
     6001 ccatgaaata gcgagcgacg actgcggcat ggtagctggt gccgcaggcc acgatgtgta
     6061 cgttggcagt ccgtttgaag acctcggtcg cggcagggcc gaaggctgct tcgagcaatc
     6121 tgccgtgcgc gacacgctcc tgcagcgtgt tggcgatggc acggggttgc tcgtggatct
     6181 ccttgagcat gaaatgccga tacggtccct tctcggccgc atccgcggac aaatcacttt
     6241 cgcgaactgg acgctcgacc cggttaccct ccttgtcgag gatccggacg ctcctgcggc
     6301 cgatctccgc gacatcgccc tcctccagaa agatgaaacg gcgcgtcacc ggcagcaggg
     6361 ctgcaacgtc ggatgcgacg aagttctcct cgacgccgag gccgatgacg accggacaac
     6421 cctcacgcgc caggatgatt cgctcgggat cccgctcgct caccaccgcg agcgcatagg
     6481 cgccctccag ctccgcgacc gtggcgcgaa cggctttgaa caggtccttc accgtctgca
     6541 agtggcgatg aatgcgatgc gcaatcacct cggtgtcggt ttccgaggaa aactggtagc
     6601 ccgagcgaac cagctcctcg cgcagctcat catgattctc gatgatgccg ttatgaacga
     6661 tcgcgagccc gtcgcgggaa atatgcgggt gagcgttgcg ttcgctcgga ataccgtgcg
     6721 tggcccatcg ggtatgggca attccaacct tgccatcggt cggctcctgc tcgagcgcct
     6781 cgtccagcat tcgcaccttg ccgacggtac gcagccgctt cagatgctgc gttccgttca
     6841 gcaccgcaat gccggcggag tcatatccgc gatactccag ccgacgcagc ccctccatca
     6901 gaatggggac gatgttgcgc tcagcgactg ctccgacgat tccgcacata gcctggattg
     6961 cctcttttcc gcctgacgtc ctctatgggg acaaagctgg cctgccagtt tgcctgaaac
     7021 gccgggtgac aggcgaattg ccctggctga agcgtggcaa agcctacgtt atgggaaaaa
     7081 gccgcctccg ggaggggcgc ccagagctcg cgaatccggt tgcagcgcaa gccctccgct
     7141 ggccaatccg acaagcgaga ggctagagtg ggggatgctg attgaaaaat cgacccgacc
     7201 ccctggaaca cccctgtcgg tgcgcctcct cctcaccagc ctgatgctgt gcgcactgtc
     7261 ctcggtggga ctggccgatc agtccaagcc ggatgccgcc gccacgatcc gcaagacact
     7321 cgaggctcgc tatcccggca cgcacgtgct cgatgttcaa ccctcggcca tggcaggggt
     7381 ctacgaagtc tttttgggcg atgagattgt ctacaccgat gccagtgcag actacattct
     7441 catggggccg atggtggaca cacagacccg caaggacctg acggaagcac ggatgaacga
     7501 gcacggccgc atcgacttcg acttactgcc actcgcccga gcgatcaagg tcgtcaaggg
     7561 caatggcagc cgtcgctttg cggtgttctc ggacccggat tgcccattct gtcagcagct
     7621 cgagaagtca ctcctgccga tcagcaatgt gaccatgtat gtgttcctct atccgatcgc
     7681 tgcgctgcac ccgcaggcac ctgccaaagc tcacgccatc tggtgcgcca aggatcgggc
     7741 ccaggcctgg aatgaatgga tgcatgagaa aaagttgccg gcgaccggtc agtgtgccgg
     7801 ggatcctgta gcggatctgc agaagctggg ggacacgctg cgcatcaaca gcaccccaac
     7861 gctcttcttc gcaaacggcc gccgggtggc cggggccatt tccaccgcgg agatcgagca
     7921 gttcctgagc gcctcggaca ttcagaagcc ggtcgcgccg gccggcaagt aaactcaact
     7981 ggggccggcg ccggcgcgtt acttcggctt actgggccgc tgccagttct cgattgtgat
     8041 ctgacgagcg cgcgcaaccg tgagcttgcg cggcgggacc tgtttcacga ccgtcgagcc
     8101 tgccccgacc gtagcgcccg cgccgatggt gatgggtgcc accaacacgc tgttcgagcc
     8161 cgtgtgtacg tcgttctcaa tcgtggtgcg atgcttgttg gccccgtcgt agttcgcaat
     8221 gatcgtcccc gctccgatgt tgacgcgctc gccgagctgc gcatccccga cgtaggccag
     8281 atgattggcc ttcgattccg ctcccatgcg cgaattcttc acttcgacga agttgccgat
     8341 gtgcacgcca ccggcgagcg tggaggtggg tctgaagcga gcgaacgggc cgatgttgca
     8401 gttcgggccg atcaccgtgc tgtcgatcac gcagtggcca aaaacttccg tgtcatctcc
     8461 gatctcactg ttgcgaatca cagtattggg tccgatgcgg acgcgatcgc ccagcttgac
     8521 gtggccttcg atgatcacgt tgacatccag gaccgcgtcc cgaccgtggg tcagcgaccc
     8581 gcggacgtcc acgcgctcag gatcggcgat tgtgaggccc gcgagcatga gctgccgcgt
     8641 gataccgcgc cggtaggcgg tctcgagggt cgcgagctgc gccttgtcgt tgacaccgag
     8701 cacctcggtc acgcgctcgg ccaccagtgg ctgaacggcg aggccctcct tgacggccat
     8761 cgcgatgaca tcggtcaaat aatactcgcc ctgcgcattg tcgttggtca cggctttgag
     8821 ccacttgcgc aaggatctgc ccggtgcggc aagtaccccc gtgttgcatt ccgtcagccg
     8881 cagctccttc ttcgaggcat ccttctgctc gacgatgcgc cgcacctggc cacgtgaatt
     8941 gcgaaccacg cggccatagc cggtcggctc ttccaggatc accgtgagga gcgccgcctt
     9001 tctgggtccc gcgatcgaca cgagctccgt gagggtggag cggccgatca gcggcacatc
     9061 tccgtagagc accaggacca gcgcttcgtc aggtacctgt ggcatggctt gcagcacggc
     9121 gtggccggtg ccgagccgct ccgcctgcaa tacccaggaa atacccggtt cctgcagctc
     9181 ctcccggacg cgctcgcccc catgtccata gaccacgtgc agggccttcg gctcgaggga
     9241 gcgtgcggtg tcgatgacat gcttcaggag cgggcggcca gcaagcggct gcagcacctt
     9301 cggcaggtca gaatgcatgc gcttgccctg tccggccgcg agaatcactg cgtgaagggg
     9361 caggctgtct ttctttgcca tgttgcttgg gtccgtgaag cgcccggagc tcattacctg
     9421 gcgatgattg tagcgtgacg agagcgcccg cccgcgcccc cctcgagggc agccagctgc
     9481 tgacggatcg cggtgccggc ccagatctgg ggattggaat gcacctcgtg ctcggcagct
     9541 tcccggcgct ggatgatccc cgtcgccttg cggaaagcgt cctccagggt acggctcccg
     9601 ggcacggagt cctgcaggaa cgcctcgccg aaccaggtga ggtcccgcgc atcgtcacag
     9661 ccgaaggagc tgtggttgga atcggctgcg gtcatgacga gcgtgtcatc actcttcagc
     9721 ggatccagga acacccccgc atagcaggcc gacacgatca cgacacgcca cttgatgcct
     9781 gagtcgtcga gcgcctgccg caaatccgcc ggtgcaagct gctgtaaggg cagagatcca
     9841 ttcacgatcg ccagcccgtc ccgcgagcca tgcgaggtga gcaggagaac gagaacgtcc
     9901 tgctcggtgt ccatgtgcga ggccacgagc ctgagcgcct ggccgagggc cgaaacgctg
     9961 gcgatgggat aggagtcgcg gtcgttgtcg tcgttgatga gctcgatgga gtgatcgccc
    10021 gagccgaaat gatccgcgaa cacctgttct gcgtagagcg cctcgcgctt gaaaatgccc
    10081 tgctcaccat ctcctgcgaa gccgacgaag aatacgttcg gctcgccagg ctggcgcgcc
    10141 gccatgtgct cgacggagcc ggcgatacgc ccgggctggt catatagcaa cgcctccgcg
    10201 gacgtatcgt ccgattgctc atcctggctg tccgccgtga gccacagtcg cgtatccaga
    10261 ttcaggctct gcagcgccca cggcgcggcg gcgataaaaa ccgccgcaag caggacggcc
    10321 gtacgggatg gagttacaaa agccgactgc aggacgcgga cgcccagcaa cagcaggtaa
    10381 agagtccccg caaccagagc gcccagggga tggctgctga cctgatgcag atcgctcagc
    10441 agccagaatg cgatcaggac gtaaggcgag gcggatagcg ccgggatgag cacagcgcga
    10501 gtgtccgcgg accgactctg cgcgcgggcg accagtccac aggcaaacaa tccgaccagc
    10561 aggtagaacg cccacgtgta caggccatcc agcatgagct gcgatccggc ttcggcgtgc
    10621 aaggtatcga gcgcagccca gacgagcaga ttgatgagca ccagcgccgc gagctgatcg
    10681 aagctgcgca caaagcgctc cgcgggcaag cgccgcaacc cgcaaagtcc caagccgctc
    10741 cgcaggttct ggagcagacc cgaacgtaga gtggcaaagg ctggagcgct cgcgttcagc
    10801 tccgcagttt gcgcaaccgc tcgatcagtt tcagctgtgc ggccgctcgc gccagctcgg
    10861 cctgagcatg cgccaggtcg atgtggccgg tcttgtcctg cagggcttcc tcggcgcgct
    10921 gtttggcctc gagcgcggcg gcctcatcca gatcgcgcgc gcgcgccgcg gtgtcggcga
    10981 ggacagtgat cttgtgcggt tgcacttcga gcactccgcc gccgacgaag taggaatgct
    11041 cctgcccgtc cggtgtttta acgcgcaatt cgcccgcttt gagcaggctg agcagcggtg
    11101 cgtggcgagg caggatccct agatcaccca tgcatcctga cacgagtacc atcgcggccg
    11161 gacctgcgaa cagctcgcct tcggcgctga cgatgtgaag atcaaccgtg ctctgctgcg
    11221 tctcggccat gaccactctc tactgcagcg acttcgcctt ggcgaccgct tcctcgatac
    11281 cgccgaccat gtagaaggcc tgttcgggga gcgagtcgaa ttcgccgccg atgatgccct
    11341 tgaagccgcg aatggtctcc ttgagcggga cgatctttcc atcctgcccc gtgaacacct
    11401 tggcgacgtt gaaaggctgt gacaggaagc gctgcacttt gcgcgcccgg tacacggtca
    11461 gcttgtcgtc ttccgacagc tcatccatcc cgaggatcgc gatgatgtcc tgcagctcct
    11521 tgtagcgctg cagcacggcc tgcacgccgc gcgcggtctg atagtgctct tcacccacga
    11581 ccagcgggtc gagctggcgg ctggtcgaat cgagcgggtc cacggccggg tagatgccca
    11641 gtgaggcgat ctgccgggac aggacgacgg tggcatccag gtgcgcgaac gtcgtcgccg
    11701 gtgaaggatc cgtcaagtca tcggcaggga cgtacacagc ctggatcgaa gtgatcgagc
    11761 ccgttttggt cgaggtgatc cgctcctgca gcacgcccat ctcctcggcg agcgtcggct
    11821 ggtagcccac cgccgaaggc atccgaccca gcagcgccga tacctcggtg cccgccaggg
    11881 tgtaacgata gatgttatcg acgaaaaaca gcacgtcccg gccgcgtccg ccatcttcgc
    11941 gcttctcatc acggaaatat tccgccatgg tcaagcccgt gagcgccacg cgcaggcggt
    12001 tgccgggcgg ctcgttcatc tgcccgtaaa ccatcgcgac cttcgagttg gacaggttgt
    12061 ccttgtcgat gacacccgac tcgatcatct cgtgatagaa gtcgttgccc tcgcgggtgc
    12121 gctcgccgac gcctgcgaac accgatagtc cgctgtactg cttcgcgatg ttgttgatca
    12181 gctcgagcat gttgaccgtc ttgccgacgc cagcgccgcc gaacaggcca accttaccgc
    12241 ccttcgcgaa cggcacgagc aggtcgatca ccttgatgcc ggtgaccagc aggtcctgac
    12301 cgcctgcctg atcctcgaac gagggcgggg gacgatgaat ggggagatgc tcttttgcct
    12361 gcacctcacc ccggttgtcc tgggggcgcc ccagcacgtc catgatccga ccgagcgtac
    12421 ccttgccgac cggcacggag atcggcgctc cagttgccag cacgggcagg ccgcgtttca
    12481 gaccttccga ggagcccatc gcaatcgtgc gcacgatgcc gtcgccaagt tgctgctgca
    12541 cctcgagtgt cagatcggca tccttgagct tcaaggcctc gtacacgtgc gggatggact
    12601 cgcgtggaaa ggcgacgtcg atgacggcgc caatgatctg cgtgatcttg ccttcgacgg
    12661 catgggtcat ggattgaagt cctctgcatg aaaggatggc gcgcgaatca tactgccgct
    12721 gcgcctccga cgatttcgga caattccttg gtgatggcgg cctggcgggc cttgttgtag
    12781 acgagctgca gctcgctgat cagcttgccg gcgttgtccg tggcggcctt catggccacc
    12841 atgcgggcag ccatctcgga agcgacgttc tcgacgaccc cgcgataaac ctgagattcc
    12901 acgtaccgca tcaacagccc gtccagaata tcgcgcgcat cgggctcgta gatgtagtcc
    12961 caatgctcgg gaagattctc ggtatcgcga ccctccacgg gcacgagctg ctcgaccgtg
    13021 ggcttctggg tcatggtgtt caggaaatgt gcgttgacca ggaatacgcg atccaccttc
    13081 ccctcgcggt acgcatccag catgaccttc accgtaccga tcagatcctt gatgtggggc
    13141 tgatcgccga ggccggaggt gttggcgagg atcggcacac gcagccggcg aaagaagttc
    13201 agccccttcg tgccgatgat gcacaggctc acctgtgcac ccttctcctg ccactcacgc
    13261 atcaggtgga tcgtctgtcg gaagacgttg gcattcagtc cacccgccag gccgcgatcc
    13321 gtggagacga tgatgatgcc gatgttcttc ggctcgcgtg cgaccagaaa cggatgcttg
    13381 aaatcgggat tcgcatccgt gagatgccca atcacgttgc gcatctcctg cgcgtagggt
    13441 cgcgcctgct tcatgcgctc ctgcgcacgc cgcatcttgc tggtggccac catctgcatg
    13501 gccctggtga tcttctgagt gctcttgaca ctcgcgatct tggtgcggat ttccttggtg
    13561 ccgggcatgg ctggggggcg gcctcagtag gttcccgtcg agacgaagtc ctcgatgcac
    13621 ttcttcaatg ccgcttcgtt ctccttggtg agcttcggat tggcgttgac gctgtcgagc
    13681 atctgccggt agttggtatc ggcgaatgcc tgcaggcccg cctcgaaatc gaccaccttc
    13741 ttcagctcga tcttgtccat gtagccgttg ttgaccgcgt agatcgataa ggccatctgg
    13801 gcaaccgaca tcggtgtgta ctgcttctgc ttcatgacct ctgtggtccg ctggccgcgc
    13861 tcgagctgcc tgcgggtcgc ctcatccaga tccgaagcaa actgcgagaa ggccgcgagc
    13921 tcgcggtact gcgccagggc gaggcggatg ccgccaccga gcttcttgat gatgtcggtc
    13981 tgcgccgcgc cgcccacacg cgagaccgag atgcccgcgt tcatcgccgg acggatgccg
    14041 gcgttgaaca gatccgtctc cagaaagatc tgtccgtccg tgatcgagat gacgttggtc
    14101 ggcacgaacg ccgtcacgtc gccggcctgg gtctcgatga tcggcaggcc ggtgagagag
    14161 ccggtcttgc ccttgacggc gcccttgctg accatctcga catactcttc gttgacccgt
    14221 gcgctgcgct cgagcaggcg cgagtgcagg tagaacacgt cgcccggaaa tgcctcgcgg
    14281 cccggaggcc gccgcagcag cagcgagatc tgacgatagg cgaccgcctg cttggacaga
    14341 tcgtcataga tgatgagcgc gtcctcgccg ttgtccatga agtactcgcc catggtcacg
    14401 ccggaatacg cggagatgta ctgcagagca gccggactcg aggcggaggc cgccacgacg
    14461 atcgtgtgcg ccatcgcacc ctgctcttcc agcttccgga cgacgtttgc gacggtggac
    14521 tgcttctgtc cgatcgcaac gtagatgcag cgaatgccgg agtccttctg attgatgatg
    14581 gtatcgacgg cgagcgcggt cttgcccgtc tggcgatccc cgatgatcag ctcgcgctgt
    14641 ccgcggccga tcggcaccat cgagtcaaca gccttgtagc cggtctgaac cggttggctc
    14701 acggacttgc gatagatgac acccggggcg acgcgctcga tcggggctgt gcgtgcggcc
    14761 ttgatcgggc ccttgccatc cagcggctcg cccagcgcgt ccacgacgcg gcccagcaac
    14821 tccggaccca ccggtacctc aaggatgcgg cccgtggtct tgaccgtggc gccctcgacg
    14881 aggtccttgt actcacccag cacgacggcg ccgacggaat cctgctcgag gttcagagcc
    14941 agtccgaaga cgttgccggg aaactcgagc atctcgccgt agcgcgcatc ggcaagaccg
    15001 tgaatgcggg tgatcccgtc ggtcaccgac acgatggttc cgacgttgcg ggcctccgta
    15061 acgcctttga aattctcgat ccgctgtttg atcagatcgc tgatttcggt tgccttgatg
    15121 gccatgtcag tatcctatgc gaattcgtta tgcggtcagc tcgtaggcga tgcgctcgag
    15181 gcgcgcgcgc agggaaccgt cgatcacgag atcgccggcc cgcaggaccg cgccgccgat
    15241 cagcgcggga tcgacgtggc actgcacgcg gatctggcgc ttgagccgcc tctgcagcgc
    15301 ggtggacagc gcttcgagct gcccgttcac aagtggcgcg gcggaagtga ccgtcacgtc
    15361 gaccacgccc tcggcctcgt tcttcagctg atcgaagaga gccgagatct ccggcagata
    15421 agccaggcgc cgattgtcag ccagcgtgcg cacgaagttg cgaccggtct cgtccagttg
    15481 gggtccggcc aggtcggcga cgaatgcggc cagatccgct ttgcggacgg caggattgcc
    15541 gagcagccgc ttcacacgag ggtcttgcac cacggtagcc gccgcccgca gagtctcgga
    15601 ccagcggccg agggtcttcg cacgctgtga ctcctcgaac gccgccttcg catagggtcg
    15661 tgcaatggtt gtcctgtcag ccatggcctg tccggtccag tcagatctgc gccgcgagct
    15721 tgccgagcag gtccgcgtgg gctttcggat cgatctcacg ctcgagcagt ttggaagcgg
    15781 cgctgacggc aatctcggcc acttcctgcc gcagtgactc gcgggcccgc gtggtgtcca
    15841 gctcgatgcg ctgatgcgca gcctcgacca gccgctggcc ctcgaccgtg gccgcacctt
    15901 tggcatgctc cacgagatcg ttggcgcgat gctgcgcctg atcgatgatc tggttggccc
    15961 gttcacgcgc ctcgcggatg atcgtctccg accgctcgcg cgcctgcgcc agctcctgct
    16021 gtcccttctc cgcggccgcg agcccctgcg cgatcttgcg cgagcgctcc tccatggcac
    16081 ccaggatcat tggccacacg aatctcatcg tgaaccagat gaatagcgca aagacgatca
    16141 tctgaatgat gagcgtcagg ttgatattca cgctcgtctc ctatatctgc acgcgccggc
    16201 aacagccgcc cgcacgggat tcagtgcgca gctgcctgca cggcggtcag gaacgggttc
    16261 gcgaacatga agaagagcgc gaaggcgatg ccgatgaagg cgaccgcatc gagcagtgcc
    16321 gcgacgatga acatcctggc gagcaacgcc ggcgcgagct ccggctggcg cgccatgcct
    16381 tcgaggaagc ggccgccgag gatgccgaaa ccgattgccg tgccgaacgc gcccgagccg
    16441 aaaatgatgc cgcacgcgag cacggtcatg gcctggatgt gtgcgatgtt ttccattcaa
    16501 ctctcctgac agatattcga aattggccgc cccgcctgct gcaagctcag tgatgctcgg
    16561 acgcctgcga gaggtacacg atggtcagca tcatgaaaat gaaggcctgg agcagcgcga
    16621 tgaacgtgtc gaacagcgac cagagcacac cgagggccgc ctgcagcagg ccccaccccc
    16681 agcttcccgg ggacgtcagc gactcgaggc ccctcgagag ggtgatcagg gcgagcagcg
    16741 caaacaccat ctcgcccgcg tacatgttgc cgtagagccg cagcgcgagc gagaccgggc
    16801 gcgcgatgaa gctcggaaat tccatgacga aattgaccgg cacgagcagc gcttgcagca
    16861 gcacgttctt gctgctgaac ggatgcaacg tgaggctgcc gacgaagccg ccgaacccct
    16921 tcatcctgat gctgtagata atgatgagca ggaagacggt gatcgagagt ccgaacacga
    16981 tgttgagatc ggtgctggga acaatcttga gatgcccgag acccgcaccg tgcgctgcgg
    17041 agggcagcag atcgatcggg atcacgtcca tcaggttgaa gcacagcacc cagccgaaga
    17101 tggtgagcga gaggggggcg atcagcttgc tctggccctg gaaagtggtc ctgacctggg
    17161 tgtccacgaa ctcgacgatc cattcgacga agttctggaa cttgccgggc acgccggtgg
    17221 tggccttgcg ggcagcggcg tagaaggcgc cgccgaatac gaccgcaagc aggatcgaga
    17281 aaaatacact gtccagattg acgacggaaa gatcgagtat cccccgcggt gccacattgg
    17341 tgaggaaggt gatgtggtga gtgatgtact cggtaggact ttgagcttct gccgccgcgc
    17401 gtgccaggaa catatgttta tcgcatctct cgcaaaatca gcctcgcagc ccgcgaagcc
    17461 gacgcaaccc accgagcccc ggcacgaagc ctgcgagcac gatccagtac accaaaaaag
    17521 tcgctccgta agctgcgagc atcgcagccg gcgcgggcct caacgtggtg aggacgagcg
    17581 tgaacagcgc gatcaccacc accagcttcg ccagctcgcc cacgtagaag gcggcgagca
    17641 atcgctcagc gcttcccgag gcccatcttc cgaaggcgag aaccgtcatc gcgaggctgg
    17701 cagcggcggc aatccctccg cccagcaggg ccgaaacggc cgaattccaa cctgccagga
    17761 gccagcaaag acaggcgacg atcagcgtcg caaccacctg ccccaacacg acaccgaagg
    17821 ccagtcggcg cgcatcggcc agttcaatcg cgaccatctc aatccccgcc cgcgcaggcg
    17881 agcgccacta cttcccagcc tgacaatatg aggcgcgcgg ctcgctttta aattaccagg
    17941 acgtaagcga cgctcacccg ggcgagccgg cttgcgcggc ctacaccccg ccgccgcctg
    18001 agcctgtgtg gcctcgaggg cgcagcccgg tcaaaaaagc cgcgggatta tagagaccgc
    18061 ggccctttca gtccacagca cggcgtggtg gttttcgccg cggaccgcgg cacgtggaaa
    18121 gtgttccaga gatgacgcct ggcagctcga tcaagggctt atgaggggcc ggccgtcagt
    18181 ggatgtgcgc cagaataccg tcgagttcat ccagcgtgtt gtagctcacg accagcttgc
    18241 ccttgcctgc cgtcgtatgt tgaatcgcga cccgggcgcc aagcttgtcc gccagctcct
    18301 gctccaggcg gtgcacattc ggatcgccgg gcggggccgt ctcgggcgcg acggccggct
    18361 gctgcatgcg ccgcaccaaa gcctcggtgt cgcgcaccga cagccccttc gtgacgatca
    18421 gagtggacac ctcgacctga cgctggcgat tggtgagagc aaggagtgcc cgggcatggc
    18481 ccatctcgat ctcccggcgc tcgacccgat ccgagacctc gggcgcaagc tcgagcaatc
    18541 gcagcagatt cgtcacccct gcccgggagc ggccgacggc atccgccgcc tgctgatggg
    18601 tgagggtgaa ctccgtgatc agccggtcaa gagcgcgcgc ttcctccagc ggattgaggt
    18661 tctcccgctg gatattctcg atcagggcga cggcgatcgc cgcctcatcg ggaatccgcc
    18721 ggat
//
LOCUS       DASRHN010000319         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2089904, whole genome shotgun sequence.
ACCESSION   DASRHN010000319 DASRHN010000000
VERSION     DASRHN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089904"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFB37_12595"
     CDS             <1..782
                     /locus_tag="VFB37_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZO23333.1"
                     /translation="ENSDGAGPADTSQMKQAQDLKQASEKQQMLEHKQLESLMAELRA
                     AVNKSQALQPFKDQLLLDITPEGVRIQIVDAQNRPMFDVGSARLRDYTSAILRELAPY
                     LDSVPNRISLTGHTDIRPYPSSNGYTNWELSADRANAARRALTAAGLPDSKISRVVGL
                     SSSVLFDKADPQNPINRRISIVVMTKQAEEDALKTDVAKESVAPGTTAASAAPDAAAP
                     ASSVPPPASAAPVSAAPIPATPVPAAPSRAVPATAAARPGT"
     gene            complement(1055..3295)
                     /gene="mdoH"
                     /locus_tag="VFB37_12600"
     CDS             complement(1055..3295)
                     /gene="mdoH"
                     /locus_tag="VFB37_12600"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HZO23334.1"
                     /translation="MGGRASGELPVMTPERLRSAARRRRALFFSLTFLTSAFASWLMY
                     DILRANGFTPLEKASLALFFVLFTWITGAFWTALFGFVIRLRGRDRAVIHADEVSGRV
                     LRSRTAVIMPIYNEETQRVIAGLEVIWSSLQRQPQQAAFDFFILSDTRKSDIAAAEEV
                     AWRSLVERRGAHGRIFYRRREDNIGRKAGNIADFVRSWGGAYEYAIVLDADSIMSGDA
                     LVALSLMMDAHPEAGIIQALPMPAGRETLFARLVQFAARLNSLMLASGLAYWQLGEGN
                     YWGHNAILRLRQFAEFCDLPRLPGAPPLGGEILSHDFVEAAFMRRAGFQVWLLADLGG
                     SWEEVPSNVIDYAARDRRWAQGNLQHLGLLPTRGLHWLSRLHLITGVMSYVTSPIWLL
                     VLTLSSIIVCLDALHGFQYFEPGSYTLFPSWPESRATEIVALLAITLGVLLMPKVLGA
                     TLALVKPDLRRGYGGAFRLISSVLVEQIFSTLLAPAMMLFHTTFVVSILAGRPVSWNT
                     QERGDRGIGFLEALNRHKWHILLALIWGAALLAITPRYFYWMVPILGGLILSVPLTMF
                     TSRASVGRWMRHRGLLLTPEETNPPAELRALEERLKIEAGEQRPRSLPGRDLGRREPV
                     LQMAELALASDRHVGDTLVARAANGFHANGAGMNGSGLNGSQTNGSDANGSGRHAVTM
                     NSEIGGDPDTQEGEPEIQGIVPLTPIPERVPLQMQAVRLVYLRPRDALSGLQRLWGGP
                     RTGTSL"
     gene            complement(3297..>4448)
                     /locus_tag="VFB37_12605"
     CDS             complement(3297..>4448)
                     /locus_tag="VFB37_12605"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HZO23335.1"
                     /translation="AELGFAGFRVHYPLNVPNYKDEVLVFLGASYFRVLGRNQSYGLS
                     ARGLAVNTATEAGEEFPYFTDFWLVRPEPDQHSLTLFALLDSPSVTGAYQFELRPGSI
                     TQVQVSGELYPRKTIEKVGIAALSSMFLYGENYTGRHFDDYRPEVHESDGLMTQTGVG
                     EWAWRPLVNPRSLRVNRFMDEHPRGFGLIQRDREFAHYQDAAAQFQTRPSYWIEPQGD
                     WGRGGVELVEIPSDEEIHDNIVAYWVPDTPVQAHKAISFSYVLYAFTHSNQWPPGGRV
                     ISTRTGAVATTPSAQSATGLRWMIVDFAGGDLDDLYANQPVQAHITANGGDIDDVTVQ
                     RLPQSAIWRASFKLKPNGEKPVDMRCYLTLYGEALTETWTYLWKPAGGA"
BASE COUNT          846 a         1499 c         1394 g          709 t
ORIGIN      
        1 aggagaactc cgacggcgcc ggcccggccg acacgtcgca gatgaaacag gctcaggacc
       61 tgaagcaagc cagcgagaag cagcagatgc tcgagcacaa gcagctcgag tcgttgatgg
      121 cggagctgcg cgcagccgtc aacaagagcc aggcgctgca gcccttcaag gatcagctcc
      181 tgctcgacat cacaccggaa ggcgtgcgca tccagatcgt cgatgcgcaa aaccgcccca
      241 tgttcgatgt cggcagcgcg cgtctgcgcg actacacgag cgccattttg cgcgagctcg
      301 ccccctacct cgattcggtg cccaatcgca tcagcctcac cggacatacc gacatccgtc
      361 cctacccgag ctccaacggc tataccaact gggagctgtc cgccgaccgc gccaatgccg
      421 ctcgccgcgc actcacggcc gccggcctgc ccgacagcaa gatctcccgc gtggtcggac
      481 tgtcctcgtc cgtcctgttc gacaaggccg acccccagaa cccgatcaac cgtcgcatca
      541 gcatcgtggt gatgacgaag caggccgagg aggacgcgct caagaccgac gtcgccaagg
      601 aatcggtcgc ccccggaacc accgcagctt ccgcggctcc cgacgctgct gccccggcct
      661 cgtcggttcc gccgcccgca tctgcggccc cggtctctgc ggcgcccatc cctgcgacgc
      721 ccgtccctgc ggctccgtcc cgggcggtgc cggcgaccgc agccgctcgc ccgggcactt
      781 gatcttcgag cgcacaactt caaggcacac cgcggcagct cgacccatcc cggtgcgtga
      841 acctgacgtc acgctcgagg agggccacgg tcaatcagag acttacggaa cagccgctcg
      901 gtggagccgc tggcattgga tttgctttga ttgccgcgag gcatgtttga acgcaaccga
      961 ctcttgaaag gcgggaaggt tgctggcaac gcggtgcgtg ctcgcggcaa ccccctcgcc
     1021 gcgggcacgc accgcgcatt ttgactgcgc ggcgctagag agacgtgcca gtccggggtc
     1081 caccccacag gcgctgcagg ccgctcaggg catcgcgcgg acgcagataa acaagccgca
     1141 cggcctgcat ttgcagcggg actcgctccg gaatcggtgt cagtggaacg atcccttgaa
     1201 tctccggctc gccttcttgc gtatccggat cgccgccgat ctcgctattc atggtgacag
     1261 cgtgccggcc tgacccattc gcatccgagc cattcgtctg cgatccgttc aacccggacc
     1321 cgttcatgcc ggccccgttg gcatgaaacc cattcgcagc tcgcgcaacg agcgtgtcgc
     1381 cgacgtggcg atctgaagcc agcgcaagtt ccgccatctg cagaaccggc tcccgcctgc
     1441 cgagatcccg ccccgggagg gagcgcggcc gttgctcgcc agcctcgatc ttgagccgct
     1501 cctcgagtgc cctgagctct gccggcggat tcgtttcctc cggcgtcagc agcaagccgc
     1561 ggtggcgcat ccagcgccca acactcgcac ggctggtgaa catggtgagc ggtaccgaca
     1621 gaatcagacc gccgaggatc ggcaccatcc agtagaaata gcgcggtgtg atggccagca
     1681 gcgcagcacc ccagatgagg gccagcagga tgtgccactt gtggcggttg agggcctcga
     1741 gaaatcctat cccacgatct ccccgctcct gcgtgttcca tgacaccggc cggccggcga
     1801 ggatgctgac gacgaatgtc gtgtggaaca gcatcatcgc tggcgccagc agggtgctga
     1861 agatctgctc gaccaggacg ctggaaatga gcctgaaggc gcctccgtag ccgcgacgca
     1921 gatcgggctt gacgagtgcg agcgtggctc cgagtacttt cggcatcaac agcacaccga
     1981 gcgtgatcgc cagcaacgcg acgatctcgg tggcccgcga ttcgggccag cttgggaaca
     2041 gggtgtagga gcccggctcg aagtactgga agccgtgcag cgcatccagg cacacgatga
     2101 tcgagctcaa ggtgagcacg aggagccaga tgggcgaggt cacatacgac atcacgccgg
     2161 tgatcagatg gagccggctc aaccaatgca ggccacgggt cggaagcagc ccgaggtgct
     2221 gcagattgcc ttgcgcccag cgccgatcgc gggcggcata atcgatcacg ttggaaggga
     2281 cttcttccca gctcccgccg agatctgcga gcagccagac ctggaagccc gcgcggcgca
     2341 tgaatgccgc ctccacgaag tcgtgactga ggatctcccc gccgagcggc ggcgcgccgg
     2401 gcagccgggg cagatcgcag aactcggcga actggcgcag ccgcaggatg gcgttgtggc
     2461 cccagtagtt gccctcgccg agctgccagt aagcgagacc gcttgccagc atcaggctgt
     2521 tgagccgggc ggcaaactgc accagccgcg cgaacagcgt ctcgcgcccg gccggcatcg
     2581 gcaatgcctg aatgatgccc gcctccggat gagcgtccat catcagggac agcgctacga
     2641 gcgcatcgcc gctcatgatg ctgtcggcat cgagcacgat cgcatattcg taggcgccgc
     2701 cccaggagcg cacgaagtcg gcgatgttgc ccgccttgcg tccgatgttg tcctcgcgcc
     2761 ggcgatagaa aatacgcccg tgggcgccac gtcgctccac cagcgatcgc caggcgacct
     2821 cctcggcggc ggcaatgtca gatttgcgag tgtcggacag aatgaagaaa tcaaacgccg
     2881 cctgctgcgg ctggcgctgc agcgaggacc agatcacctc gaggccggcg atgactcgct
     2941 gagtttcctc gttgtatatg ggcatgatca cggcggtgcg gctgcgaagc acccgcccgc
     3001 taacctcatc ggcgtggatt accgcgcgat cgcgcccgcg caggcggatc acgaagccaa
     3061 acagcgctgt ccagaaagcc ccggtgatcc aggtaaacag cacgaagaac agcgccagac
     3121 tcgccttctc caggggagtg aatccattgg cgcgcaggat gtcgtacatc agccagctgg
     3181 cgaacgccga ggtgaggaag gtgagagaga agaacagcgc acgccggcgg cgcgctgcgc
     3241 tgcgcagccg ctcgggtgtc atgaccggga gctccccgga cgcacgaccg cccatgctat
     3301 gcaccccccg ctggcttcca cagataggtc catgtctcgg tcaaagcctc accgtagaga
     3361 gtgagatagc accgcatatc gaccggcttc tcgccattgg gcttcagctt gaaggacgcc
     3421 cgccagatcg cagattgtgg cagccgctgc acggtgacgt catctatatc ccctccattt
     3481 gccgtaatgt gggcctggac gggctgattc gcgtacagat catccagatc gcctccggcg
     3541 aagtcgacaa tcatccagcg caggccggtt gcagattggg cgctgggggt cgtcgccacg
     3601 gcccccgtgc gggtcgagat cacgcggccc cccggcggcc actggttgga gtgggtgaag
     3661 gcataaagca cgtacgagaa cgagatggcc ttgtgggcct gtaccggggt atccggcacc
     3721 cagtaagcca caatgttgtc gtggatctcc tcgtcggagg gtatctcgac cagctcgaca
     3781 ccgccccggc cccagtcgcc ctggggctcg atccagtagc tcggccgggt ctgaaattgt
     3841 gccgcggcat cctgatagtg ggcgaattcc cggtcgcgct ggatcagccc gaagccgcgc
     3901 ggatgctcat ccatgaaccg gttcacccgc agcgagcgcg gattgaccag cgggcgccag
     3961 gcccactccc ctaccccggt ctgagtcatg agtccgtcgc tctcatggac ctccggccgg
     4021 taatcatcga agtgccggcc cgtgtagttc tcgccataca ggaacatcga gctcagggca
     4081 gcgatgccga ctttctcgat cgtcttgcgc ggatagagct cgccgctgac ctgtacctgg
     4141 gtgatggacc cgggtcgcag ctcgaactgg tacgcccccg tgacgctcgg gctgtcgagc
     4201 agcgcaaaca gcgtcaggga gtgctggtcc ggctcgggac ggaccagcca gaaatcggtg
     4261 aaatacggaa actcctcgcc ggcctccgtc gcggtgttca ccgcgagccc gcgtgccgac
     4321 agcccgtagg actggttgcg gccgaggacc cggaaataag aagcaccgag gaacacgagc
     4381 acctcatcct tgtaattggg cacattcagg ggatagtgga cccggaaccc cgcgaaaccc
     4441 agctcggc
//
LOCUS       DASRHN010000320         9956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_603632, whole genome shotgun sequence.
ACCESSION   DASRHN010000320 DASRHN010000000
VERSION     DASRHN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603632"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1100)
                     /locus_tag="VFB37_12610"
     CDS             complement(132..1100)
                     /locus_tag="VFB37_12610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZO23336.1"
                     /translation="MSPLVSIIVPTFNRLTYLRKSLASVFAQTFRDWELLIADDGSGI
                     DTRTYLQSLQDPVRVRVLWLPHTGRPAVARNAALRAAKGEYVAFLDSDDLWTPTKLQK
                     QMDSLRSRSNRRWGYTRFVLVDGSGNPTAWQCARSWPVPEGWIFDKLVRSETVIAVPS
                     VVASRELLQQVGGFDESLVMCEDYDLWLRLAAQSEVDVIDEPCTLVTRHAEHSGSELI
                     SFEDCARVFAKVRKTRGTEHLHAILNEKSAEVAIGLARSQVACGDRLSGLRTLIASAP
                     QSWRYRRWWLGTLITTARALAPQPVLQMLRSRRPASAGRARGVPQP"
     gene            complement(1088..2302)
                     /locus_tag="VFB37_12615"
     CDS             complement(1088..2302)
                     /locus_tag="VFB37_12615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO23337.1"
                     /translation="MASSFPHVAQISFFNDPADRHPVALLDAWPTLVAVAEAACAAGV
                     RVSVIQASTHSQELTRNGVDYYFLPFGAATPMAGAAEPLGTLFRRLSPNVIHVHGLGF
                     PRHVLDLAQVAPGIPIVLQDHADRPPRIWRRALWRRAISRAAAITFCSAEQARPFQAV
                     GLISSGARLYAIPESTSRFTPGDREQARQLTGIAGDPAILWVGHLNANKDPLTMLAGI
                     SVAARTLPGLKVWCCFGSAPLEAEVRRHIEGDPLLTDRVHLLGKVPHAAVERLMRAAD
                     LFVLGSHHESTGYSVIEALACGLPPLITDIPSFRSLTAGGEVGMLWQPGDSRAFCDAL
                     QLIAGRLGPGMRAGARLHFERELSLSALGSKLAEMYRDQLQIAGGKQDLQAWPAHEGG
                     LGPTGGEVSCRR"
     gene            complement(2308..3822)
                     /locus_tag="VFB37_12620"
     CDS             complement(2308..3822)
                     /locus_tag="VFB37_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO23338.1"
                     /translation="MSNARRILLINPTITGKRHARFPLAVMSLSAALEGRHTTRILDG
                     NIDRDFVATALRAVEQDQVDAVGVTVMGGPQLTSAIAVSKAIRERRPAIPIIWGGAFP
                     TVCPDAAVSVPYVDYAVRAQGEETINELLDALSGGHRTDLGSIAGLTRRVDGEVVHNR
                     DRLFSGASLKRMLPYGQLENPQQYLTHTYLGQRTMGYQAALGCRFRCTFCGVATMFRG
                     KTALPPAARLEEDLTFLTKQLGVNAVQFYDHNFFDREVDMVPLLEILARFELSWWCFA
                     RSDALVNLSESSWALVKKSRLRMAYIGAESPSDWLLHDIRKGTRSDQTMEAIEACRRH
                     GVIPELSFMLAPPQDPEGETERTFDFIRAIKRAHPATEVMIYIYTPLPQRPGSKYAAA
                     VRLQSEMRDCAGRPIVFPETAEEWAAPQWLGYWCHQDAPWLSARLRRRIVDFTTVLGC
                     RFPTVMDIRASAWGKSALRALASWRYRFQRYDDPWELRASRKIIRHWDPRVMSL"
     gene            complement(3819..5249)
                     /locus_tag="VFB37_12625"
     CDS             complement(3819..5249)
                     /locus_tag="VFB37_12625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO23339.1"
                     /translation="MSNTSPRRKVLLYNPQAVFFTMPLALLAIGSELDPALYEVIIVD
                     GRLDPDPEKTLRSHIDEAVCLGVTVLTGAPISDALRISRAAKRARADLPVVWGGWHPS
                     MFASECLLEPSVDVTVRGQGEETFVEIVARLVRSQSLLGCAGCTVRLADGSIHENPPR
                     PLAPVDRFRAHDYGLIPVERYYELKGKRQLDYISSQGCNFRCAFCSDPFVYGRKWVGL
                     EPTRMALRLKELWDRYRFDDVNFQDETFFTKARRVQALAERIVESGMKITWAATMRAD
                     QGIRLPEEVWALCKQSGLRRLLVGVESGSNEMLKRIRKDIRIEQVYQVAETMLRHEIA
                     GHFPFIVGFPEESDASIQATLDCAKRLRSMSPDFQTPIYYFKPYPGSELVIEAVARGF
                     RLPDTLEAWSEFDYVAGLPGPWVSPEKFRLIERFKFFHELAWKRVYLGKALLQRLARY
                     RCRRDNYQWPVEMLLERWVLPAQRLS"
     gene            complement(5246..5983)
                     /locus_tag="VFB37_12630"
     CDS             complement(5246..5983)
                     /locus_tag="VFB37_12630"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZO23340.1"
                     /translation="MLDLTDAALTPEVRGSLRPSRFPVFSRLAGKVLTYSYRLLGRHR
                     YDDYRLERVLGAPLVVMPSVFNPKLPRTGEFLARQLTPAFPGGDAEVLDMGTGSGVGA
                     VIAARHARRIVAVDINTEAVRCAGINVRLNRVEGRVEVRQGDLFGPVRDERFDLILFN
                     PPFLRGTPSDDRERAWRSSDVAERFAAGVAQHLKPAGAALVLLSTFGGAEHFLEQFRR
                     QALAIAVHAERRFVNEKLVIFRLTSLA"
     gene            complement(5976..7016)
                     /locus_tag="VFB37_12635"
     CDS             complement(5976..7016)
                     /locus_tag="VFB37_12635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO23341.1"
                     /translation="MTAPVVPAASAVLQRLPLVTLYLTERCNSRCVTCDYWRHGRADL
                     NLDSVQQLLPQLLELQTRVALISGGEPLLNPEWEQIARLLRAHGLKLYLLTSGLSLAK
                     HAARAARLFDAITVSLDGTDAQTYAAIRGLDAFDKVCEGTALAASEGVSVSLRVTLQR
                     ANYRQIPKFVHLARTLGASEVSFIAVDIANPHAFAREGDFSSDLALAPEDLPVLERIL
                     CTLEREQAADFESGLIAESPRRLRHILKYFSAVCGKGAYPPVRCNAPEFSAVINARGQ
                     VNPCFFIPGPASSHWRGGLQSALNDEPMRTLRGRIRAGARPECARCVCSLWRDPERRE
                     VPDFLLQRNAHA"
     gene            7233..8111
                     /locus_tag="VFB37_12640"
     CDS             7233..8111
                     /locus_tag="VFB37_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZO23342.1"
                     /translation="MSPRGFPFVAGSLALALSACAGNGMGLDANGNPITAGGGSTGPL
                     TADFKSIQDNVFTPICTKCHIGAGAPEGLQLDQTHSYAMLVGVPSAEQPNVLRVAAGD
                     PDSSYIVLKLQGSAGISGARMPFGGPYLPQSTIDVIRQWISAGAANSTSATNAATAKA
                     AQRFTVTASSPDPDSIATTVPNIVVAFSSDVDPNLLNATMVSLEKLDASNMDSTEPMP
                     MSPMPMSPVPAGAASRSVAITLAVPHGDGTALLITPRTLLSSGTYRLTIRGNFADMNA
                     QALGADYVLKFTVEAP"
     gene            8108..9190
                     /locus_tag="VFB37_12645"
     CDS             8108..9190
                     /locus_tag="VFB37_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23343.1"
                     /translation="MTRLLRFLWVPVFFSALCAHAEPYLAVQMGLKCGQCHVNPTGGG
                     ERTAFGNIFAQTQLAATRLDTGSDVWTGQVMKFLSIGGDVRFDATYSQVPHASSTDDF
                     EVQQGRVYASANVIPERFYVYVDEQVAPGGALNREAYGVYWSANHDWYIKAGQMYLPF
                     GFRLQDQSAFVQQISGINMTTPDQGLEFGFEHGNWDAQVAASNGTAGGPETDHGKQYS
                     AQLQYVMSRWRFGVAGNFNDASAGSKSASGLFAGLRTGPISWLGEVDVVDDRSTPTLS
                     GRQLATLLEANWRIAQGHNLKLTDEYLDPNRSVANDAETRYSLVYEMTPIQYVQIRAG
                     FRWSDGIPQDPAEHLKTGFVELHGFF"
     gene            9430..>9956
                     /locus_tag="VFB37_12650"
     CDS             9430..>9956
                     /locus_tag="VFB37_12650"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZO23344.1"
                     /translation="MSDLKQLLGVDLPLIQAPMAGVQDSALAIAVSNSGALGSLPCAM
                     LDAPTMRRELAAIRAQTPHPVNVNFFCHAPPSQSPEQEARWRAALEPYYREYGIETTQ
                     VRSGATRKPFTAEALEVLREFRPAVVSFHFGLPSTELLAGLRSWNATILSSATTVQEA
                     LWLEAHGAHAIIAQGL"
BASE COUNT         1868 a         3246 c         3174 g         1668 t
ORIGIN      
        1 gcgatgatca gttcccagtc cgagagcgtc tgggcaaata ccgactcgat ggccgcgggg
       61 agaaactcca gccggttgta cgtcggcatg atgaccgaca cggcaggtgc tggagagagg
      121 gcgtgcatga gctagggttg cggcacgccg cgcgcgcggc cggcgctggc cggtctgcgg
      181 ctgcgcagca tctgcaaaac cggctggggg gcaagcgccc gcgcggttgt gatcagcgtg
      241 ccgagccacc agcgccggta tcgccaggac tgcggagcac tcgcgatcag agtgcgcaag
      301 ccgctgaggc gatctccgca ggcgacctga cttcttgcca acccgatggc aacctcggct
      361 gacttctcgt tcagaatcgc atgcagatgc tcggtgcccc gcgttttccg taccttcgcg
      421 aagacccgcg cgcagtcttc aaaggagatg agctcgctgc ccgaatgctc cgcatggcgc
      481 gtgacgagcg tgcacggctc atcgatgaca tcgacttcgc tctgggcggc aagacgcagc
      541 cacagatcat agtcctcgca catgacgagg ctctcatcga accctcccac ctgctgcagc
      601 agctcgcggg acgccaccac gctgggcacc gcgatgaccg tctccgacct caccagcttg
      661 tcgaagatcc agccctcagg aaccggccag ctgcgggcgc actgccaggc agtgggattg
      721 ccggagccgt ccacgagaac aaatcgagtg tagccccatc tgcggttgga acggctgcgc
      781 agggagtcca tctgcttctg cagcttggtc ggcgtccaga ggtcatccga gtcgagaaag
      841 gcgacgtatt caccctttgc tgcgcgcagc gccgcattgc gtgccacagc cggcctgccg
      901 gtgtggggca accaaagcac gcgcacccgt accggatcct gcagcgactg cagatacgtc
      961 cgtgtgtcga tgccggagcc gtcatcggcg atcagcagct cccaatcccg aaacgtctgg
     1021 gcaaacaccg acgcgaggct cttgcgcagg tatgtcagcc gattgaacgt gggcacgatg
     1081 atggagacta gcggcgacac gagacctctc cgccagtggg gccgagcccc ccctcatgag
     1141 ccggccacgc ttgcagatcc tgcttcccgc ccgcgatctg cagctggtcc cggtacattt
     1201 ccgcaagctt tgaccccagg gccgacagag acaattcgcg ctcgaagtgc aggcgtgcgc
     1261 ccgcgcgcat cccaggtccc agtcgcccgg cgatgagctg taatgcatcg cagaatgccc
     1321 gtgagtcacc cggctgccag agcatgccaa cctcgccacc cgcagtgagt gaccggaacg
     1381 aggggatgtc cgtgatcagc ggcggcaagc cgcaggccag cgcctcgatg accgagtatc
     1441 ccgtgctttc atgatggctg ccgagcacaa agaggtctgc tgcgcgcatg agccgctcca
     1501 ctgcagcgtg cggcaccttc cccagaaggt gcacgcggtc ggtcaacagc ggatcgccct
     1561 cgatatgacg tcggacctcc gcttcaaggg gcgcactgcc gaaacagcac cacaccttga
     1621 ggcccggcag agttcgcgct gccacactga tgcctgccag catggtcagc gggtccttgt
     1681 tggcattcag atgcccgacc cagagaatgg cgggatcgcc cgcgataccc gtcagttgcc
     1741 gcgcttgctc gcgatccccg ggagtgaacc ggctggtgga ctccgggatc gcatacaggc
     1801 gcgcgccgga gctgatgagt cccaccgcct ggaacggcct ggcctgctct gcagagcaga
     1861 acgtgatcgc cgcagcacgt gatatcgccc tccgccagag cgcgcgccgc cagatgcgcg
     1921 gcgggcgatc ggcatgatcc tgcaggacga tcggaattcc cggggcgact tgcgccaggt
     1981 ccagcacatg gcggggaaat ccgagaccgt gcacatggat gacattcggt gacagccggc
     2041 gaaacaaggt gccgaggggt tccgccgccc cagccatcgg cgtggcagcc ccgaagggca
     2101 ggaagtagta atccacgccg ttacgggtca actcctgcga gtgtgtggat gcctggatga
     2161 ccgagacgcg aacccctgcg gcgcaggcag cctcggcaac ggcgaccaga gtcggccacg
     2221 catcgagcaa ggcaaccggg tgccggtccg cagggtcgtt gaagaagctg atctgggcga
     2281 cgtgtgggaa ggagctcgcc atggtgctca caagctcatg acgcgcgggt cccagtgacg
     2341 gatgatcttg cgcgaggcgc gcagctccca cgggtcatcg tagcgttgaa accggtaacg
     2401 ccaggacgcc agggcgcgca gtgcgctctt gccccaagcc gaggctcgga tatccatgac
     2461 ggtcgggaag cggcagccca gcaccgtcgt gaagtcaaca atgcgccgcc gcagtcgcgc
     2521 cgagagccac ggggcatcct ggtgacacca gtagccaagc cactgcggcg cggcccattc
     2581 ctctgcggtt tccggaaaga cgatcgggcg tccggcgcag tctcgcatct cgctttgcag
     2641 gcgcacggcg gccgcgtact tgctccccgg gcgttgtggc agcggcgtgt agatatagat
     2701 catgacttcg gtcgccgggt gtgcccgctt gatggcgcgg atgaagtcga acgtccgctc
     2761 cgtctcccct tcgggatcct gcggcggggc cagcatgaac gacagctcgg gaatcacgcc
     2821 atggcggcga caggcctcga tggcctccat cgtttgatcg gagcgcgtgc ccttgcggat
     2881 atcgtgcaac agccagtcgc tcggcgactc ggcgccgatg taggccatgc gcaggcggct
     2941 cttcttcacc agcgcccatg agctctccga cagattcacg agcgcatccg agcgggcgaa
     3001 gcaccaccac gacagctcga accgcgccag gatctccagc agcgggacca tgtccacttc
     3061 gcgatcgaag aagttgtggt cgtagaactg aaccgcattc acgcccagtt gcttcgtcag
     3121 gaatgtcagg tcctcctcga gccgtgccgc aggcggcagt gccgtcttgc cgcggaacat
     3181 cgtcgcgacg ccgcagaacg tgcagcggaa gcggcacccg agggccgcct ggtagcccat
     3241 ggtgcgctgg ccgagatagg tatgggtcaa atactgctgt ggattctcca gctggccgta
     3301 gggcaacatc cgtttgagac tcgcgccgct gaagagccga tccctgttgt gtacgacctc
     3361 accgtccacg cgccgggtca ggccggcaat cgatccgaga tcggtccggt ggccgccgga
     3421 caaggcatcg agcaactcgt tgatcgtctc ctcaccctgt gcgcggacgg catagtccac
     3481 atagggaaca ctgaccgccg catcaggaca gactgtgggg aacgctccgc cccagatgat
     3541 cggtatggcg ggccggcgtt cgcgaatcgc tttggatacg gcgatcgccg aggtgagctg
     3601 aggcccgccc atcacggtca cgcccacggc atcaacctga tcctgctcca ccgcgcgcaa
     3661 cgcagtggca acgaaatccc gatcgatatt gccatcgaga atacgtgtcg tatgccggcc
     3721 ctcgagcgcg gcggacaggc tcatcactgc aagcggaaag cgcgcgtgac gcttaccggt
     3781 gatggtcggg ttgatcagga gaatgcgcct cgcattgctc atgagagcct ctgggcaggc
     3841 agtacccagc gctcgagcag catctcgacc ggccactggt agttgtcacg tcgacagcga
     3901 tagcgggcga gccgctgcag cagggctttg cccaggtaaa cgcgcttcca ggcgagctcg
     3961 tgaaagaact tgaagcgctc gatgagcctg aacttctcgg gagagaccca ggggcccggc
     4021 agccccgcga cgtaatcgaa ctccgaccag gcctcgagcg tgtccggcag tcgaaagccg
     4081 cgcgcgaccg cctcgatgac cagctcgctg ccgggatagg gcttgaagta atagattggt
     4141 gtctgaaagt cgggactcat cgagcgcagc cgcttggcgc aatcgagcgt cgcctggatg
     4201 ctggcatcgc tctcctccgg aaagccgacg atgaagggaa agtggccggc gatctcatga
     4261 cgcagcatcg tctcggcgac ctgatagacc tgctcgatac ggatgtcctt gcggatgcgc
     4321 ttgagcatct cgttcgagcc ggactcgacc ccgaccagca ggcggcgcag gccggactgc
     4381 ttgcacagcg cccacacctc ctcgggaagg cggataccct gatccgcgcg catcgtcgcg
     4441 gcccaggtga tcttcatgcc ggattcgacg atccgctcgg caagcgcctg cacgcgacgc
     4501 gctttggtaa agaaggtctc gtcctggaaa ttgacatcat cgaagcgata ccggtcccac
     4561 agctctttca aacgcaaggc catgcgggtc ggctcgagcc ctacccactt gcggccgtaa
     4621 acgaacggat ccgagcagaa ggcgcagcgg aaattgcagc cctgtgagga gatgtaatcg
     4681 agctggcgct tgcccttgag ctcgtagtag cgctcgaccg ggatcaggcc gtagtcgtgg
     4741 gcacggaaac ggtcgaccgg ggcgagcggc cgcggcggat tttcatggat gctgccatcc
     4801 gccagacgca cggtgcagcc ggcgcacccg agaagcgatt gactgcgcac cagccgagcg
     4861 acgatctcaa cgaaagtttc ctccccctgg ccgcgtaccg tgacatctac cgagggctcg
     4921 agcagacatt cgcttgcgaa catcgatgga tgccagccgc cccataccac cggtaggtcc
     4981 gcccttgcgc gcttggccgc ccgtgagatt cgcagcgcat cggagatcgg tgcacccgtc
     5041 agcacggtga caccgagaca gaccgcttcg tcgatgtgag accgcagcgt cttctcgggg
     5101 tcaggatcca gacggccatc gacgattatg acttcgtaga gagcgggatc cagctcggaa
     5161 ccgatggcga gcagtgcgag cggcatcgtg aagaagaccg cttgcgggtt gtagagcagc
     5221 accttgcgcc gtggactcgt gttgctcatg cgaggctcgt caggcgaaaa atgaccagct
     5281 tttcgttcac gaaacggcgc tcggcatgca ccgcaatcgc cagcgcttga cggcggaact
     5341 gctcgagaaa gtgctcagca ccgccgaatg tcgaaagcag caccagtgct gcgcctgcgg
     5401 gtttcagatg ctgcgctacc cccgcagcaa accgctcggc cacatcgctc gagcgccagg
     5461 cacgctcgcg atcatcgctg ggcgttcccc gcagaaaagg tggattgaac aggatgagat
     5521 caaagcgctc atccctgacg ggcccgaaga gatcgccctg acgcacttcc accctgccct
     5581 ccacacgatt gagtcgtaca ttgatgcccg cgcagcggac cgcttcagtg ttgatatcga
     5641 ctgccacgat gcgacgcgca tgccgtgcgg cgatcacggc acccactccc gatccggtgc
     5701 ccatatccag cacttccgca tcgccgcctg ggaaagcggg tgtcagctgg cgggccagaa
     5761 actcgcccgt acgcggcagt ttcggattga agacgctggg catgaccacg agcggggctc
     5821 cgagcacgcg ctccaggcga tagtcgtcgt agcggtgccg gcccagcagg cggtagctgt
     5881 atgtcagcac cttgccggcg agccgcgaga acaccggaaa gcgcgagggg cggagcgagc
     5941 cgcgtacttc cggcgtcagg gcagcgtccg tcagatcaag catgggcgtt gcgctgcagg
     6001 agaaagtcag gcacctcgcg gcgctccgga tcgcgccaca gggagcacac acatcgggcg
     6061 cattcgggcc gggcaccggc gcgtattcgc ccgcgcaggg ttctcatcgg ctcatcgttg
     6121 agcgcgctct gcaaaccgcc tcgccagtgg ctgctggcag gccctggaat gaagaagcag
     6181 ggattcacct gccctcgcgc attgatgacc gccgagaact ccggcgcatt gcagcggacc
     6241 ggaggatagg cacccttccc gcagaccgca ctgaaatact tcaggatgtg ccgcaggcga
     6301 cgcgggctct ccgcaatcag cccggactca aaatcggccg cctgctcgcg ctcgagggtg
     6361 cacaggatcc gctcgaggac cggcaggtcc tccggcgcaa gagccaggtc cgagctgaag
     6421 tctccttcgc gggcaaacgc gtgtggattg gcaatatcca ctgcgatgaa ggacacctcg
     6481 gatgccccca gcgtgcgcgc caggtggaca aacttcggga tctgccggta gttggcacgc
     6541 tgcagcgtga cccgaaggct cacggacacc ccctcgctcg cggccaaggc ggtgccttca
     6601 cacaccttat cgaaagcatc cagaccccga atcgctgcgt aggtctgcgc atcggtcccg
     6661 tccagcgaga cggtgatggc gtcgaacagg cgggcggctc gcgcggcgtg cttggccaga
     6721 gacagacctg aagtcagcag gtagagcttg agaccatggg cgcgcagcag tcgggcgatc
     6781 tgttcccatt ccggattgag cagcggctcg ccgcctgaaa tcagcgcgac gcgcgtctgc
     6841 agctcgagca gctgcggcag cagctgttgc accgaatcaa ggttgagatc ggcgcgtcca
     6901 tggcgccagt agtcgcaggt cacgcagcgg gaattgcagc gctcggtcag atacagagtg
     6961 accagcggca gacgctgcag cacggcggaa gcggctggca cgaccggagc ggtcactggc
     7021 agggctcgta cggacagata tcccccggtc gggcgtggag aaaggttctc gatgcgtgag
     7081 tgacgcgttc ccgttcccgg gttcaatcga ggctgcagcc agcgacgccc gatcccgcat
     7141 cccggccctc acagaaaaaa atcgtacagg gccgaaacct tgcgcccgcc gctgcggcac
     7201 ttacggctgt ctcaattgcg tgagcattcg caatgtcccc gcgcgggttc cccttcgtgg
     7261 ccggctccct ggcccttgcc ctgtccgcct gtgccggcaa tggaatgggg ctggacgcca
     7321 atggcaatcc catcacggca ggcggtggca gcaccgggcc cctgacggcc gacttcaaat
     7381 ccatccagga caatgtcttc acgccgatct gcaccaagtg tcacatcggc gccggcgcgc
     7441 ccgaaggcct gcagctcgat cagacgcaca gttacgccat gctggtgggc gtgccgagcg
     7501 cggagcagcc gaatgtcctg cgcgtggcgg cgggcgatcc cgattccagc tatatcgtgc
     7561 tcaagctgca gggaagtgcg ggcatcagtg gcgcccgcat gcccttcggc ggcccgtatc
     7621 ttccccagtc cacgatcgat gtgattcgcc agtggatctc ggcgggcgcg gctaactcga
     7681 cgagcgcaac caatgccgcc acggccaaag ccgcgcaacg cttcacggtt acggcctcct
     7741 cacccgaccc ggattccatc gcgacgaccg tgccgaacat cgtcgtcgca ttcagcagcg
     7801 atgtcgatcc caatcttctc aatgccacga tggtgagttt ggagaagctg gacgcctcga
     7861 acatggactc gacggagccg atgcccatga gtccgatgcc gatgagcccc gtgccagccg
     7921 gggcggcttc gcggtcggtt gcgatcaccc tcgcagtccc ccatggggat ggaaccgccc
     7981 tgctcatcac gccccgcacc ctgctcagca gcggcaccta tcgcctcacc atacgaggca
     8041 atttcgccga catgaatgca caggcgctcg gtgccgatta cgtcctcaag ttcaccgtgg
     8101 aagctccatg acgcgcttac tgcgtttcct gtgggtgccg gtctttttca gcgccctctg
     8161 cgctcacgcc gagccgtacc tcgccgttca gatgggattg aagtgcggtc agtgtcacgt
     8221 caatccgacc gggggcggcg agcgcaccgc ttttggcaac atctttgcgc aaacccagct
     8281 cgccgcgacg cgtctcgata ccggatcgga tgtgtggacg gggcaggtca tgaagttcct
     8341 gtcgatcggc ggtgatgtgc gcttcgatgc cacgtattcc caggtgcctc acgcctcctc
     8401 caccgacgat ttcgaggtcc agcagggccg tgtctatgcg agtgcaaacg tcatcccgga
     8461 gcgcttctat gtctacgtgg acgagcaggt cgcacccggt ggcgcgctga accgcgaggc
     8521 ctatggcgtg tactggtctg cgaatcacga ctggtacatc aaagcgggcc agatgtacct
     8581 gcccttcggc ttccgcctcc aggaccagag cgccttcgta cagcagatct cgggaatcaa
     8641 catgaccacg cccgatcagg gcctggagtt tggcttcgag cacggcaact gggacgcgca
     8701 agtcgcggcg agcaatggca ccgccggggg tcccgagacc gatcacggca agcagtacag
     8761 cgcgcaactg cagtacgtca tgtcacgctg gcgcttcggc gtcgcgggca atttcaacga
     8821 tgcctccgca gggagcaaga gtgcgagcgg gctgttcgcg ggcctgcgca ccgggccgat
     8881 ctcctggctc ggtgaagtgg acgtggtcga tgaccgcagc acccccaccc tgagcgggcg
     8941 tcagctcgcg acccttctgg aggcgaactg gcgtatcgca cagggtcaca atctcaaact
     9001 caccgacgaa tacctcgatc ccaatcgcag tgtcgccaac gatgccgaga ctcgttacag
     9061 cctcgtctac gagatgacgc cgatccagta cgtgcagata cgcgccggct ttcgctggtc
     9121 ggacggtatc ccgcaggacc cagccgagca cctgaagacg ggattcgtcg agctgcatgg
     9181 gttcttctga gattgcagaa agtcgcctac ggcgctttct gcccgcgggc gagcgccctg
     9241 tcacgaccct tggcgacgct gcaaaaggcg tggcttttgc agctcaacgc tccgcgctgg
     9301 aggtctgcgt tgcgaaagtc gcctacggcg ctttctgccc gcgggcgagc gcgcaaagca
     9361 gcggagctca gacggctgtt aagatgagcc ggtcgggcgc gagacgcgcc acgcatgccg
     9421 gagagacttg tgtcagacct gaagcagctg ctcggcgtgg acttgccgct gattcaggcc
     9481 ccgatggcgg gcgtccagga cagcgcgctg gcgattgcgg tatccaacag cggcgcgctc
     9541 ggatcccttc cctgcgcgat gctggatgcg ccgacgatgc ggcgcgagct ggctgcgatt
     9601 cgcgcgcaga ctcctcatcc cgtcaacgtt aactttttct gccacgcgcc gccgagccag
     9661 agcccggagc aggaggcacg ctggcgcgcg gcattggagc cgtactatcg ggagtatgga
     9721 atcgagacga cgcaggtgcg ctcgggagcg acccgcaagc cctttacggc ggaggctctc
     9781 gaggtgctgc gggagttccg ccccgcggtc gtgagctttc acttcggact gccttccacg
     9841 gagcttctgg ctggcctgcg ctcctggaat gccacgattc tgtcctcggc caccacagtc
     9901 caggaagcgt tgtggctcga ggctcatggt gctcacgcca tcattgccca aggtct
//
LOCUS       DASRHN010000321        12610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1434164, whole genome shotgun sequence.
ACCESSION   DASRHN010000321 DASRHN010000000
VERSION     DASRHN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434164"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFB37_12655"
     CDS             <1..181
                     /locus_tag="VFB37_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZO23345.1"
                     /translation="ARSDLHSGDLIVGVNDAPVDSIDVLHRELSRWPLGTALTLNIVR
                     RTQSLQISLTPREPS"
     gene            375..4838
                     /gene="gltB"
                     /locus_tag="VFB37_12660"
     CDS             375..4838
                     /gene="gltB"
                     /locus_tag="VFB37_12660"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HZO23346.1"
                     /translation="MSPAYTGLHKEAYERDSCGFGLIAHLDDEPSHWLVRTAITSLNR
                     LTHRGAVAGDGKTGDGCGLLLKRPERFLRAIALEAAIELTDLFASGLVFLSQEPEAAS
                     QAKRMLENELARQGLETAGWRAVPLNTDACGEQALKSLPRIEQIYVNCPLPELDEASF
                     NRKLYLGRRHAEKHLRASDRAFYVPSLSASTIVFKGMVMPQYLTRFFPDLEDPRLESS
                     VAVFHQRFSTNTLPQWRLAHPYRYLAHNGEINTVQGNRNWALARGPLFRSPLLPELAD
                     VQPLVSLTGSDSQSLDNMLEVLLMGGLDALHAMRLLVPPAWHGLDSIDADLRAFYEYY
                     SVHMEPWDGPAGIVLTDGRYAACTLDRNGLRPARFVLTRDRILTIASETGVWDYAPGE
                     VVRKGKLGPGDMIALDLQTGTLLESQDIDQILKARYPYKAWLKKGVRYLETDLVDPRL
                     AAEPMDRETLALYQKMFNVTSEERDEIIRVLAEDESEAVGSMGDDTPMPVLSHRVRSL
                     YDYFRQQFAQVTNPPIDPLRESIVMSLQTQIGPECNIFVPAPEHARQIVLGSPILSQR
                     KLRQILGLDEVTHEFIDLQYAASEGLRQAILRMCRQAESAVLEGKQVLLLSDRYLIKG
                     RLPAHALLVTGAVHQHLVRTGLRCKCNLLVETGTAREPHHFACLIGYGATAVYPYMAY
                     QVLFDMMRKGRVKLDFTTRLELGRSYRAGLRKGLFKIMSKMGISTIASYRSSQLFEIV
                     GLASEVVDLCFTGTESRVQGADFADLESDLSQLAARAWNACEPIDQGGLLKYMHGGEY
                     HMYNPEVVATLQGAVIAGDYERYRLFAQLVNQRPASTFRDLLGLRAAAQPVPLGEVEP
                     LEAILARFDSAGMSLGALSPEAHEALATAMNRLGARSNSGEGGEDPARYGTERNSKIK
                     QVASGRFGVTPEYLINAEVLQIKIAQGAKPGEGGQLPGHKVNDMIASLRFARPGVGLI
                     SPPPHHDIYSIEDLAQLIFDLKQVNPAALVSVKLVAEPGVGTVAAGVAKAYADLITIS
                     GYDGGTGASPLSSIKYAGTPWELGLAEAHQTLRINELRQRVRLQADGGLKTGLDVVKA
                     AIIGAESFGFGTAPMVALGCKYLRICHLNNCATGVATQHNVLRSKYFTGLPEMVINYF
                     RFVAQEVRELLALLGVRKLEEIIGRTHYLQILEGQTPRQRKLDLTPILGTDALAQEAP
                     QYCSAPSNVPFDKGELAERMVRDMLPAIESKSGGEWAYEVCNYNRSIGARLSGEIARR
                     WGNYGMEDAPLTVRLVGNAGQSFGVWNAGGLQLYLEGDANDYVGKGMAAGKIVLRHSR
                     QARFVTHQTVIMGNTCLYGATGGQLFAAGVAGERFAVRNSGAVAVIEGAGDHCCEYMT
                     GGVVCVLGRSGVNFGAGFTGGFAYVLDLQRDFVDRYNHELIDISRIHAESMQSHLQHL
                     EGLLEQHVAETGSLWAEEIRNDLRTYIGKFWVVKPKAASIDSLMENLRRAA"
     gene            4887..6299
                     /locus_tag="VFB37_12665"
     CDS             4887..6299
                     /locus_tag="VFB37_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO23347.1"
                     /translation="MADSNLQFLDIPRADPAKDPVEHRIRHFGEIYSPYDAESAGQQA
                     GRCLACGNPFCEWKCPVHNYIPNWLKLIEEGNLFEAAELSHKTNSLPEVCGRICPQDR
                     LCEGACTLNDGHGAVTIGSIEKYITDEAFRRGWKPDMSNVRPTGKRVAVVGAGPAGLG
                     CADILVRNGVRPVVFDRYERIGGLLTYGIPPFKLEKRIVEKRREVMEAMGVEFRLGTE
                     VGSDVPFESLLAKFEAVFLGMGTYTAVKGGFPGEDLPGVHEALPYLISNIRHELRAPG
                     SAERLVSMRGKRVIVLGGGDTAMDCNRTAIRQDAQSVTCTYRRDELNMPGSRRDYRNS
                     REEGVELLFNRQPIEIVGAERVEGVKLVETRLGRPDLRGRRVPEPVPGSEHVLPADAV
                     IIAFGFLPSPPDWLGQHDIRLHANGRVRVSAAHELAFQTTHPKVFAGGDMVRGSDLVV
                     TAVFEGREAAKGILRYLRVG"
     gene            complement(6943..8943)
                     /locus_tag="VFB37_12670"
     CDS             complement(6943..8943)
                     /locus_tag="VFB37_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO23348.1"
                     /translation="MSLRLKLLLLALATLVLPWAGCRYAKEMESALREGEQNSLSSVA
                     ETMASSLQGRRDLLYREPRTPAVAADTDEGETSSPEPEPASPYDIKPILLFAPPFLDG
                     YTDDWPQDRSAWRFFAQGRHRFGILTGVYERMLYVLLDVQDEHLVFDAPGASPLDPSA
                     FGDRVWLGFDDTHGTQHQYLVSATAPGSVTGRRIETGEYGQQNAVTEPRIVGAWMPTP
                     QGYRVELRVPLSMLGEHFGVLIDDRDARGALPVSYGTLRSDDLHTVGRLIVASPELTR
                     YLRQFIQPGQRLSIATPDGRLLAQADALAQVTALGPGTGLLARLYRRFVDRPNARRIV
                     EATASIYDRDHTRIVGSLKVSQTEDRWLTLRDRALTRMLNSTLLISAIAVVAILLFAA
                     RLAMRLSRLRAASESALTRAGLVTTFPETGAADELGDVARGFSTLLSRLNEYTGYLRT
                     LAAKLAHEIRTPLTIVRSSLENLESEGLSPAAGVYLERAREGSQRLNAILIAMGAATR
                     VEEAISGAERTRFDLVPILTSAAASYRIAFPERHFAIELPAEPVEIEGAPDLIVQMLD
                     KLVDNAVDFSPAGSTITLRLRLEPQAAILEVDNPGPPLAGAAQSRLFESLWQSRTGGD
                     SRPHFGLGLYIVRLIAEFHGGSASAISLPQDTGARFCIRLAR"
     gene            complement(8974..9663)
                     /gene="pdsR"
                     /locus_tag="VFB37_12675"
     CDS             complement(8974..9663)
                     /gene="pdsR"
                     /locus_tag="VFB37_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HZO23349.1"
                     /translation="MKRMVAIVEDEPAIRDNYAAAFTREGYVVRAYANRAEAMTAFSA
                     RLPDLAVIDISLEEEPEGGFELCRQLRALSAELPIIFLTARDSELDAVSGLRLGADDF
                     LTKDLSLAHLIARVNALFRRVEALRRPLEASQIIRRGPLNLDGERMQVEWDGQVVLLS
                     LTEFWIVFALARHPGHVKNRQQLMDAANVVLDDNTITSHIKRIRRKFQSIDPKFDAVQ
                     TVYGMGYRWVE"
     gene            complement(10279..10752)
                     /locus_tag="VFB37_12680"
     CDS             complement(10279..10752)
                     /locus_tag="VFB37_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23350.1"
                     /translation="MNRLVCTTVLSLLAAPAFAADVGVSISVGQPGFFGQINIGAIPP
                     PPVVYAQPVVAVRSPEYVAVEPIYLHVPPGHERHWSRHCAEYHACGRPVYFVRDEWYN
                     KEYVPRYAREHRDHEDVHGRGHGHDDDHGHGHERRDDDHGRGHGDHDDDHGHGDR"
     gene            complement(10979..12157)
                     /locus_tag="VFB37_12685"
     CDS             complement(10979..12157)
                     /locus_tag="VFB37_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008002122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO23351.1"
                     /translation="MTKTSPAKLDPLDLFDVRGALSEEEMMVQDSVARLVDEKVLPII
                     QKCFEEHRFPRELVPELAALGLLGSSLHGYDCAGLNGISYGLICQQLERGDSGIRSFV
                     SVQSSLCMYPIYTFGSEEQKRRYLPGMAKGELIGCFGLTEPHGGSDPSNMHTHAKRDG
                     GDWILNGSKMWITNGAIADLAVVWARTDEGIRGFIVEKGTKGFSAPEIERKFSLRASV
                     TSALFFDHVRVPDAQILPGVVGLKGPLSCLTQARYGIAWGVIGAAQACLAQLLDYTST
                     RILFGRPLAHTQAIQIRLAEMSRQITCAQLLALQLGKLKDQELMTPSQVSLAKWNNCR
                     MALQIARDARDMLGGAGISAEYVPIRHALNLESVITYEGTETIHQLTIGRELTGHNAF
                     "
     gene            12278..>12610
                     /locus_tag="VFB37_12690"
     CDS             12278..>12610
                     /locus_tag="VFB37_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZO23352.1"
                     /translation="MKIPEILLVETIHQPGSLARVLQVIADAGLAIEHLTALRRDQGR
                     TLWEITLEMDEEADRSLYQRIDQLPTARFMGKSDRVFNRHRGGKIRTVASLAINTRQV
                     LRDIYTPGV"
BASE COUNT         2286 a         3967 c         4140 g         2217 t
ORIGIN      
        1 cgcgcgctcg gacctgcact cgggagatct gatcgtcggc gtgaacgatg ccccggtgga
       61 cagcatcgat gtcctgcacc gtgagctcag tcgctggcca ctcggcacgg ctctgacgct
      121 gaacatcgtg cggcgcacgc agagcctgca gatttcgctg acaccgcgcg agccctcttg
      181 agaagggcga tgcgcgcacc gatcgcgacc acaccgaaga gcacgcacta aagaggtgcg
      241 gtgcccgccg ccacgcgagc gcgatacgga tcgccgacgg cctcgggaga tcgctattga
      301 aagcggtcgt tgtgccggta tcatgcaatc caacaacccg actgccggcc tgcgggtcgc
      361 gagatctggc cccgatgagc cccgcctaca ccggcctgca caaagaggca tacgagcgtg
      421 acagctgcgg attcgggctg atcgcgcacc tggacgacga gccgagccac tggctggtcc
      481 gcacggcgat cacctcgctc aatcgtctga ctcatcgggg cgcggtcgcc ggggatggca
      541 agaccgggga tggctgcgga ctgctgctga agcgtcccga gcgcttcctg cgtgcgatcg
      601 cgctcgaagc cgccattgag ctcaccgacc tcttcgcatc cgggctcgtc ttcctcagcc
      661 aggagcccga ggcggcgtcg caagcgaaac ggatgctgga aaacgagctc gcccggcagg
      721 ggctcgagac ggccggttgg cgcgccgttc cactcaacac cgatgcgtgc ggcgagcagg
      781 cgctcaagag cctgccccgc atcgagcaga tctatgtgaa ctgccctctg cccgagctgg
      841 atgaggcttc gttcaatcgc aagctgtatc tgggccgccg ccatgccgag aagcatctgc
      901 gggcgagcga tcgggcgttc tacgtgccga gcctgtccgc cagcacgatc gtgttcaagg
      961 gcatggtgat gccgcagtat ctgacgcgtt tctttccgga tctcgaggat ccgcgcctcg
     1021 agagctcggt ggcggttttc catcagcgct tctcgaccaa cacgctgccg cagtggcgcc
     1081 tcgcccaccc ctaccgctat ctcgcgcaca acggtgagat caacacggtg cagggaaaca
     1141 ggaactgggc cctggcccgc ggccccttgt ttcgctcgcc gctgctgccc gagctggccg
     1201 acgtccagcc gctcgtgagc ctgaccggct cggattcgca gagcctggac aacatgctgg
     1261 aagtgctgct catgggcggc ctggatgctc tgcatgcgat gcgcttgctc gttccgcccg
     1321 cctggcacgg gttggattcg atcgatgcgg atctgcgcgc cttctacgag tactactcgg
     1381 tgcacatgga gccctgggat ggtccggccg gaatcgtgct cacggacggg cgctacgccg
     1441 cgtgcacgct cgatcgcaat ggtctgcgcc cggcccgctt cgtcctgacg cgcgatcgca
     1501 tcctgacgat cgcctcggag acgggagtct gggattacgc gccgggcgaa gtcgtgcgca
     1561 agggcaagct cgggcccggg gacatgatcg cgctcgacct gcagaccggg acgctgctcg
     1621 agtcgcagga catcgatcag attctcaagg cgcggtatcc gtacaaggcg tggctgaaga
     1681 agggtgtgcg gtatctggag accgatctcg tggatccccg gcttgcggcc gagcccatgg
     1741 atcgggagac ccttgcgctg taccagaaga tgttcaacgt cacctccgag gagcgcgacg
     1801 agatcatccg ggtgctcgcc gaggatgaga gtgaagcggt gggctcgatg ggggatgaca
     1861 caccgatgcc agtgctgtcg cacagggttc gctcgctcta cgactatttt cgccagcagt
     1921 tcgcgcaggt gaccaacccg ccgatcgatc cgctgcgtga atccatcgtg atgtcgctgc
     1981 agacgcagat cggaccggag tgcaacatct tcgtgcccgc tccggagcac gcacggcaga
     2041 tcgtgctcgg atcgccgatc ctctcacagc gcaagctgcg acagatccta ggtctcgatg
     2101 aggtcacgca tgaattcatc gacctgcagt acgcggcctc ggagggcctg aggcaagcca
     2161 tcctgcgcat gtgccgccag gccgaaagcg cggttctgga aggcaagcag gtcctgctgc
     2221 tctcggatcg ctatctcatc aaaggcaggc tgcccgctca cgcactgctc gtgaccggcg
     2281 cggttcacca gcacctggtg cgcaccggtc tgcgttgcaa gtgcaacctg ctcgtcgaga
     2341 ccggtacggc gcgcgagccg catcacttcg cgtgcctcat cggttacggc gccacggcgg
     2401 tctacccgta catggcctac caggtattgt tcgacatgat gcgcaaggga cgcgtgaagc
     2461 tggatttcac gacgcgactc gagctcggcc gcagctatcg cgccggcttg cgcaagggcc
     2521 tgttcaagat catgtccaag atggggatct cgaccatcgc cagctatcgc agctcgcagt
     2581 tgttcgagat cgtgggcctg gcgagcgagg tggtggatct gtgcttcacc ggcacggaga
     2641 gccgcgtaca gggagccgat ttcgccgacc tcgaatcgga cctgagtcaa ctcgcggcac
     2701 gcgcctggaa tgcctgcgag ccgatcgatc agggcggact gctgaagtac atgcatggcg
     2761 gcgaatacca catgtacaac cccgaggtcg ttgcgactct gcagggcgcg gtgatcgccg
     2821 gggactacga gcggtacaga ctcttcgcgc agctcgtgaa tcagcggccg gcctcgacgt
     2881 tccgggatct gctcggattg cgcgcggcgg cgcagccggt gcccctcggg gaagtggagc
     2941 cgctcgaggc gatcctggcg cgcttcgaca gtgccggcat gtccctcggg gcgctctcgc
     3001 ccgaggcgca tgaagcgctc gccaccgcca tgaaccgact cggagcccga tccaactccg
     3061 gcgagggcgg cgaggatccg gcccgctacg ggaccgagcg caattcgaag atcaagcagg
     3121 tcgcatcggg ccgcttcggg gtgaccccgg agtatctgat caacgctgag gtgctgcaga
     3181 tcaagatcgc acagggagcg aagccgggcg agggcggcca gcttcccgga cacaaggtga
     3241 acgacatgat cgcctcgctg cgcttcgcgc gaccgggcgt cgggctgatc tcaccaccgc
     3301 cgcatcacga catctattcg atcgaggatc tcgcccagct catctttgat ctgaagcagg
     3361 tgaatcccgc ggcgctggta tccgtgaagc tggtcgcgga gccgggtgta ggcactgtcg
     3421 ccgccggcgt cgccaaggcg tacgccgatc tgatcaccat ctccggatac gacggcggta
     3481 ccggggcaag tccgctctcg tccatcaagt acgccgggac tccctgggaa ctgggacttg
     3541 cggaagcgca ccagacgctg cggatcaacg agctgcgcca gcgcgtgcgg ctgcaggctg
     3601 atgggggcct gaagaccggg ctcgatgtgg tgaaggctgc catcatcggt gcggagagct
     3661 tcggattcgg caccgctccg atggtcgcgc taggctgcaa gtacctgcgc atctgtcatc
     3721 tcaacaactg cgccacgggg gtggcgacgc agcacaacgt gctgcgctcg aagtacttca
     3781 cggggctgcc ggaaatggtg atcaactatt tccgcttcgt cgcgcaggag gtacgcgagc
     3841 tgctggcgct cctcggcgtg cgcaaactcg aggagatcat cggccgcacg cactatctgc
     3901 agattctgga aggccagacg ccgcggcagc gcaagctgga tctgacaccc atactgggaa
     3961 cggacgcatt ggcacaggaa gctccgcagt actgcagtgc gccctcgaac gtgccgtttg
     4021 acaagggcga gcttgccgag cgcatggtgc gcgacatgtt gcccgcgatc gaatcgaaga
     4081 gcggcggcga gtgggcctac gaggtctgca actacaaccg ctcgatcggc gcgcgcctct
     4141 ccggcgagat tgccaggcgc tggggaaact acgggatgga ggatgcgccg ctgaccgtca
     4201 ggctggtcgg caatgccggg cagagctttg gcgtgtggaa tgcaggcggt ttgcagctct
     4261 acctggaagg cgatgcgaac gactatgtgg gcaaggggat ggctgcaggc aagatcgtgc
     4321 tgcgacactc gcgccaggcc cgcttcgtaa cccaccagac ggtcatcatg ggtaacacgt
     4381 gcttgtacgg ggcgaccggc ggacagctct tcgccgccgg cgtcgcgggt gagcgctttg
     4441 cggtgcggaa ctccggcgcg gtcgcggtga tcgagggtgc cggcgatcat tgctgtgaat
     4501 acatgaccgg cggggtggtg tgcgttctcg ggcgcagcgg cgtcaacttc ggcgcaggtt
     4561 tcaccggggg cttcgcctac gtgctcgacc tgcagcgcga tttcgtcgat cgctacaacc
     4621 acgagctcat cgacatcagc cgcattcacg cggagtccat gcagtcccac ctgcagcacc
     4681 tggagggcct gctcgagcag cacgtggccg agaccggcag tctctgggcc gaggagattc
     4741 gcaacgacct gcgcacctac atcggcaaat tctgggtcgt caagccgaaa gccgcctcga
     4801 tcgattcgct gatggagaat ctacgccgtg cggcctgagc cgacagcagc gcagcgcggg
     4861 ttccagcgac gcattccttc tcgctgatgg ccgacagcaa cctgcaattc ctggacattc
     4921 cgcgcgcgga tccggcgaag gatccggtgg agcatcgtat ccggcacttc ggggagatct
     4981 actcccccta tgacgcagag tccgcggggc agcaggccgg ccggtgcctg gcgtgtggca
     5041 atcccttctg cgagtggaag tgtccagtcc ataactacat tcccaactgg ctgaagctga
     5101 tcgaggaagg caacctgttc gaggcggccg agctttctca caagaccaac tccttgccgg
     5161 aggtttgcgg ccggatctgt ccgcaggatc ggctttgcga gggggcctgt accctcaacg
     5221 acggccatgg cgccgtcacg atcggctcca tcgaaaagta catcactgat gaggccttcc
     5281 ggcgcggatg gaagccggac atgtcgaatg tgcgcccgac cggcaagcgt gtcgcggtgg
     5341 taggcgccgg accggcgggc ctgggctgcg cggatatcct ggtgcgcaat ggggtgcggc
     5401 cggtggtgtt cgatcgctac gagcggatcg gagggctgct gacttacggt attcccccct
     5461 tcaagctgga aaagcggatc gtggagaagc gacgcgaagt catggaagcc atgggcgtcg
     5521 agttccggct cggaaccgag gtgggctccg acgtgccgtt cgaaagtctc ctggcgaagt
     5581 tcgaggccgt gttcctgggc atgggaacct acaccgcggt gaagggcggc tttcccggcg
     5641 aggatctgcc cggcgtgcac gaagcgttgc catacctgat ctccaacatc aggcacgagt
     5701 tgcgcgcgcc gggaagcgcc gagcgcctcg tcagcatgcg cggcaagcgc gtcatcgtgc
     5761 tgggcggtgg cgacacggcg atggactgca atcgcacggc gattcgccag gacgcccagt
     5821 ccgtgacctg cacctaccgg cgcgacgagc tcaacatgcc gggctcgcgg cgcgactacc
     5881 gcaacagccg tgaggagggc gtggagctgc tgttcaaccg acagccgatc gagatcgtgg
     5941 gtgccgagcg ggtcgagggc gtgaagctcg tcgagacgcg gctgggccgg ccggacctgc
     6001 gaggccggcg cgtgcccgag ccggttcccg gctccgagca cgtcctgccg gccgatgcag
     6061 tgatcattgc cttcggtttc ctgcccagcc ctccggactg gcttggccaa catgacatcc
     6121 gcctgcacgc caacgggcgc gtgcgggtgt cagctgcgca tgagctggcg tttcagacga
     6181 cccacccgaa ggtgtttgct ggaggggaca tggttcgcgg ctcggacctg gtcgtcaccg
     6241 cagtgttcga aggacgtgag gcggccaaag ggatcctcag atatctgcgg gttggctagc
     6301 cgccgcgtgc tcgacctcgg gtcggtgagg gccgccataa tcccagaagg cgcgccgcgc
     6361 ccaaaaaagc ccggccgtgc ccaaaaaaag ccccgccttt ttgggggcgg ggcacgtact
     6421 gtttcccatc ggacgacccg gttgatcgct gtcagcttac ggccgggcgc gggcaaaaat
     6481 tcaaactgaa gatcgccctt tctgcaccgg cgtgtcgcgt catcacgagc atctattgct
     6541 tgcggctgga acgttggagc tcagctccgg cttattgcaa ggccagcgtg tcagttcatg
     6601 cactaccgtc ccggacaacc acaacaagtt agcgattgtc gttacccgtg cccgcgccga
     6661 ttacccgagt ttcttgcgtt cttgtctctc cacctgtgcg ctccacgcat cattgaagcg
     6721 tgcgcgctga tccgatccct tccctcgagc ctcgacgtaa gtcagccgaa gcccaatcaa
     6781 aaccggagct tgcagcgttc cgggccatgc ttgacgagcc caggattgta cgcctttggg
     6841 ccccggatta catgtcgtat ttgtgaaaca gtgaagtcgc gtcagggcaa atccggcgct
     6901 gtagcgaccc cgacttcgct cgatctgccc ccgcagtacg gctcagcggg cgagccggat
     6961 gcagaagcgg gcgcctgtgt cctgcggcag gcttatcgcc gaggcgctac caccatggaa
     7021 ctcggctatc agccgcacta tgtaaagtcc gagcccaaaa tggggccggc tgtccccgcc
     7081 ggtgcgggat tgccacaagg attcgaacag gcggctctgc gccgctccgg ccaggggagg
     7141 tcccggattg tccacctcga gaatggcggc ttggggctcg agcctgaggc gcagggtgat
     7201 ggtggaacct gcagggctga aatcgacggc gttgtcgacc agcttgtcga gcatctgcac
     7261 gatgagatcc ggcgcgccct cgatctccac cggctctgcg ggcagctcga tcgcgaaatg
     7321 ccgctcgggg aaagcgatgc ggtaggaggc ggccgcggag gtcaggatcg ggaccagatc
     7381 gaagcgggtt cgctctgccc cgctgatcgc ctcctcgacg cgcgtagcgg cacccatggc
     7441 gatgagaatg gcgttcaggc gttggctgcc ttcgcgtgcg cgctcgaggt acacgcctgc
     7501 ggcgggggac aggccctcgg attccagatt ttccagtgac gaccgaacga tcgtgagcgg
     7561 cgtgcggatt tcgtgggcga gtttcgccgc cagggtgcgc agataaccgg tgtactcgtt
     7621 caggcgcgac agcagcgtgg agaatccccg ggccacatcg cccagctcgt ccgcggcacc
     7681 ggtctcagga aaggtcgtta ccagccctgc acgcgtcaat gccgattcgc tcgcggcgcg
     7741 cagccgtgat agccgcatcg ccaggcgcgc cgcgaacagg aggatggcca cgaccgcaat
     7801 cgcgctgatc aacagcgtac tgttcaacat ccgggtcagc gcacgatcgc gcaaagtcag
     7861 ccagcggtct tcggtttggg agactttgag cgatccgacg atcctggtgt gatcgcggtc
     7921 gtaaatggag gcggtggcct caacgatgcg ccgcgcgttg gggcggtcca cgaagcgtcg
     7981 gtacagccgc gccagaagtc ccgttcctgg cccgagagcg gtcacttgcg ccagcgcgtc
     8041 cgcctgcgcg agcaaccggc cgtccggcgt tgcgatcgag agacgctggc cgggctgaat
     8101 gaactgtcgc aggtatcgcg tcagctcggg agaggcgacg atcagccgac cgacggtgtg
     8161 tagatcgtcg ctgcgcaatg tgccatagct gaccggcaat gcgcctcgag cgtcgcggtc
     8221 gtcaatcagg acgccgaagt gctcgcccag catggacagc ggcacgcgca gctcgacccg
     8281 atagccctgc ggagtcggca tccaggcacc gacgatccga ggctcggtca ccgcgttctg
     8341 ctgtccgtat tcacccgtct cgatgcgtcg tccggtgacg gaacctggtg cggttgcaga
     8401 gaccaggtac tgatgctgtg taccgtgagt gtcatcgaag ccgagccaca cccggtctcc
     8461 gaatgcgctg ggatccagcg ggctcgcccc tggcgcatca aacacgaggt gctcgtcctg
     8521 cacatcgagc agcacgtaga gcatgcgctc gtagacgccc gttagaatcc cgaaacgatg
     8581 ccggccctga gcaaagaagc gccaggctga tctgtcctga ggccaatcat ccgtgtatcc
     8641 gtcgagaaag ggtggagcga agagcaggat cggcttgatg tcgtatggac tcgccggctc
     8701 gggctccggg gatgatgtct cgccttcgtc ggtgtcggcc gccacagccg gagtgcgcgg
     8761 ctctcgatac agcaaatcgc ggcgtccctg taaagacgag gccatggtct cggcgacgga
     8821 tgagagcgaa ttctgctcgc cttcgcgcag cgccgactcc atctccttgg cgtagcgaca
     8881 gcctgcccag ggcaacacga gggtcgccag ggcgagcagc agcagtttca ggcgcaggga
     8941 catggagagg cgggcgatac ctgagcggct tgctcactcg acccagcgat aacccatgcc
     9001 gtaaacggtc tggacggcat cgaacttcgg gtcgatgctc tggaacttgc ggcggatgcg
     9061 cttgatgtgt gaggtgatgg tgttgtcatc gagcacgacg ttcgcggcat ccatgagctg
     9121 ctgccgattc ttcacgtgtc ccggatgacg cgcgagtgcg aacacgatcc agaactccgt
     9181 caaggacagc agcacgacct gcccatccca ctccacctgc atgcgctccc catcgagatt
     9241 gagcggtccg cgacggatga tctggcttgc ctcgagcggt cggcgcaacg cctcgacgcg
     9301 ccggaagagc gcgttcacgc gtgcgatgag atgagccagg ctcaggtctt ttgtcagaaa
     9361 atcgtccgcg ccgagacgta agcccgatac ggcgtcgagc tcgctatccc gcgccgtcag
     9421 aaaaatgatg ggcagctctg ccgagagcgc ccgcaattgg cggcacaact cgaagccccc
     9481 ttccggctcc tcctcgagtg agatgtcgat gaccgcgagg tccggcagac gcgcgctgaa
     9541 ggccgtcatg gcctcggccc gattcgcata cgctcgcacg acgtagcctt cgcgcgtgaa
     9601 ggcggccgca tagttatcac gaatcgccgg ctcgtcctcg actatggcta ccatgcgctt
     9661 catggggcac aggctacagc gtgagtggcg cgggagaaag ccaagcggga gtcgattcgg
     9721 cagtgttgtg agcgggttcg cggcgcgcgg ggttgtccta cggagagggg tcgggatgct
     9781 tgttgcgtgt gggcttgccc cctggaagag agctcaggcg caagggggct gccaccacac
     9841 gcaacaagca tcctctcaag cgagggctgc cctcccagcg tcgcgatccc gaccgggcaa
     9901 ccccgagcag cgttcctcac gcgcgcagag gagccgcagc gctcaaacga ttcccaccgc
     9961 gaggagtaaa agcaggccga gcgcgttcag ccaccattcg cgtcgccgta gtcgaaggag
    10021 ggccattctt gatctccgat gtccgtgcat gggagatcat ttgaagcgct cgggcgcgag
    10081 gggcaggggc gctgaggtgg ccagctgccg atcaattgtg gcaaaaaatg gcagggggcg
    10141 ggcccgcgtc tggggccaga aacgccaaga gagccggaga cggccccggc gggtgcggcc
    10201 ttcccgccgg tttatcgcag tctccgcgct cctctcagtc gaacagaagg ctaaggccgc
    10261 gatttccggc ttcgtgcctc agcggtcccc gtgcccatga tcgtcgtcgt gatccccgtg
    10321 cccgcgccca tgatcgtcgt cgcgcctctc gtgcccatgg ccatgatcat cgtcgtgccc
    10381 gtgtccgcgg ccatgaacat cctcatgatc gcggtgctcg cgggcgtagc gcggcacgta
    10441 ctccttgttg taccactcgt cgcgtacgaa gtagaccggc cggccgcaag cgtggtactc
    10501 cgcgcaatgc ctgctccagt gcctttcgtg tccgggcggt acgtgcaggt aaatcggctc
    10561 gactgcaacg tactcgggcg accgaacggc aacgaccggc tgtgcataaa caacgggcgg
    10621 cggcggaata gcgccgatgt tgatctgccc gaagaagccc ggttgaccaa cgctgattga
    10681 taccccgacg tcagcagcaa acgcgggagc cgcaaggagg ctcagcactg tcgtacacac
    10741 taacctgttc atgattaacc cttactgtat gtcaaccgtg gtggccctca ttgcagagcc
    10801 atagcgacca ggcgatacca taatcatccg tgactgcacg gcacctgaat gcagtcctca
    10861 atttgctcaa gacgatgtct ggccgtcgct caggagcgac acgaccgggc gggcgttgga
    10921 gatttgcggc tcgtggggag gctcgcgact ctggccgccc cgcaaagtgc ccccaccctc
    10981 agaaggcgtt gtgtccagtg agctcacgcc cgatggtgag ctgatggatg gtctcggtgc
    11041 cctcgtacgt gatcacgctc tccaggttga gggcgtgccg aatgggcacg tactccgcac
    11101 tgattccggc gccacccaac atgtcgcgtg catcgcgcgc gatctggagg gccatccggc
    11161 agttgttcca tttggccagg gacacctgcg acggagtcat cagctcctgg tccttcagtt
    11221 tcccgagctg aagtgcaagg agctgcgcgc aggtgatctg gcggctcatc tcggcgagcc
    11281 ggatctggat cgcctgggtg tgggcgagag gtcgtccaaa cagaattcgg gtggaggtat
    11341 agtcgaggag ctgcgccagg caggcctggg cggccccgat cactccccag gcgattccat
    11401 agcgcgcctg agtcagacag gagagcgggc ccttcagacc gaccacgccg ggcagtatct
    11461 gcgcatccgg cacgcggacg tgatcgaaga acagggcgga ggtcacggag gcgcgcagcg
    11521 agaacttgcg ctcgatttcc ggcgctgaaa atcccttggt gcccttctcg acgatgaagc
    11581 cacggattcc ttcatccgtg cgggcccaca cgacagccag gtcggcgatc gccccgttgg
    11641 tgatccacat cttggagccg ttcagtatcc agtcgccgcc gtctcgcttg gcatgcgtgt
    11701 gcatgtttga agggtcggag cccccatggg gctcggtgag gccgaagcag ccgatcagct
    11761 ctcccttggc catgcccggc aggtagcgcc gcttctgctc ttcggagcca aaggtgtaga
    11821 tgggatacat gcacagggat gactggacgg agacgaagct gcgaatgccc gagtcccctc
    11881 gctcgagctg ctgacagatc agcccatagg agatgccatt gaggccggcg caatcgtagc
    11941 cgtgcaggga ggagccgagg aggcccagtg ccgccagctc gggcacgagc tccctcggga
    12001 accggtgctc ctcgaagcac ttctggatga ttggcagcac cttctcgtcc acgaggcggg
    12061 caaccgagtc ctgtaccatc atctcttcct cggacagcgc gccgcggaca tcgaacaggt
    12121 ccaggggatc cagcttcgct ggggaagtct tggtcacact actctccggc cttcgcacta
    12181 agatggggaa tccacatcat aaaccatgct ccgccggagg gcctccgggc ggctgtattc
    12241 aggattacca ttgcacgcac aggaaagagg ctcgcgcgtg aagattcccg aaattctgct
    12301 cgtcgaaacg atccatcagc ctggcagtct ggcccgcgtg ctgcaggtga tcgccgatgc
    12361 ggggctggcc atcgagcatc tcacggcgtt gcggcgcgat caggggcgca cgctttggga
    12421 gatcacgctg gagatggacg aggaggccga ccgaagcctg tatcaacgca tcgatcagct
    12481 tcctaccgcc cggttcatgg gcaagtccga ccgcgtcttc aaccgccatc gtggcggcaa
    12541 gatccggacg gtcgccagcc ttgctatcaa caccagacaa gtcctgcgcg acatctacac
    12601 accgggcgtt
//
LOCUS       DASRHN010000322        14229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1758101, whole genome shotgun sequence.
ACCESSION   DASRHN010000322 DASRHN010000000
VERSION     DASRHN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14229
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758101"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="hmgA"
                     /locus_tag="VFB37_12695"
     CDS             <1..1046
                     /gene="hmgA"
                     /locus_tag="VFB37_12695"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HZO23353.1"
                     /translation="LEVNPNQLRWDPLPVPEEPTDFISGLRTAGGNGSPQAQSGCGIH
                     WYVANRSMYERFFYDADGELLIVPQLGRLRLATELGLLQIEPQQVAVIPRGLRFRVEL
                     PDGQARGYVCENFGAPFRLPDLGPIGSNGLANPRDFQTPVAWYEDRVGELELIAKFQG
                     HCWSAPIDHSPLDVVAWHGNHVPYQYDLRHFNTLGSVSFDHPDPSIYLVLQSLSDTPG
                     VDAIDFVIFPPRWLAAESTFRPPWYHRNVASEFMGLITGVYDAKSEGFAPGGASLHNC
                     MSGHGPDAATFEQATRADTSRPQRIDNTMAFMFETRSVIRPTRLALESPQLQSDYARC
                     WQGLRSHFTPSSR"
     gene            1054..2376
                     /gene="fahA"
                     /locus_tag="VFB37_12700"
     CDS             1054..2376
                     /gene="fahA"
                     /locus_tag="VFB37_12700"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HZO23354.1"
                     /translation="MPLNATHDPALKSWLESANDAQADFPIQNLPFAVFRRRGRSQAW
                     RGGIAIGDCVLDLAELHTLRLVQGTAADALAACTGSTLNGFMALGGEAWSALRAAASE
                     LLRTGADQASTVRAALVPQSEVEYTLPACIGDFTDFYSSIHHATRVGRLFRPDNPLLP
                     NYKWVPIAYHGRTSSIGVSGQCFSRPAGQLMPSGASQPQLLPTRRLDYELELGILIGP
                     GNRAGCPIPIEDAEKHIFGLCLLNDWSARDIQAWEYQPLGPFLAKNFATTLSPWIVTL
                     EALEPFRVPWTRSAQDPQPLPYLDARELRNRGAVDIQLEAYLESEQMRAAGAPAHRLS
                     HSTFQSAYWTVAQMLAHHTINGCNLRTGDLLGTGTQSGPEGGQGGSLLELTAGGQQPV
                     VLASGETRTFIADGDRVSFRGWCEKPGCVRIGLGEVAATVLPANQKAK"
     gene            complement(2361..3605)
                     /locus_tag="VFB37_12705"
     CDS             complement(2361..3605)
                     /locus_tag="VFB37_12705"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HZO23355.1"
                     /translation="MRAIIATALIGALLSAGCGGGGGGSGLGSQSTQPTGPAANVQAV
                     IVDGGPTALSNTPDVNTLFVTVTVCVPGSTTSCQTIDHIQVDTGSSGLRILGSVLTLN
                     LPLHTDASGAATVECTQFVDGFSWGPLRTADLQIAGESAAGIAVQVIGDAAYPDTSAP
                     SACSSVGKAEDTVAAFGANGILGVGPFISDCGSGCSIVPNPIYYACTSSSSCQNTAVD
                     VTAQVSNPASFFATDNNGVIIELPSVGATGAASVSGSLVFGIGTQSNNGLGSAAVFTL
                     ASDGSLSTEYKGQALPESFIDSGSNGFFFTDSSIPGCPDPQQDPFYCPSSTLSLSATI
                     TGQNNTSSPVNFSIANADSLFNQSIVIAAASDLGGAGISNSSFNATQSFDWGLPFYFG
                     RHVFTAFENRNTAGGMGPYFAF"
     gene            complement(3619..4095)
                     /locus_tag="VFB37_12710"
     CDS             complement(3619..4095)
                     /locus_tag="VFB37_12710"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HZO23356.1"
                     /translation="MKRARRTSLSVWVAVTAGILSIPAWKPALAALGEDVSSVEQDRQ
                     QMRGGLQVTSAAEGYTIHAIRTPGNTVVKEYVSAAGRVFAVTWQGPLLPDLRQTLGSY
                     FAQYQEAASAPHAGHRRLRIEQPDLVVHSHGHMRAFFGEAWVPSLLPPNFSVAKIN"
     gene            complement(4268..4780)
                     /locus_tag="VFB37_12715"
     CDS             complement(4268..4780)
                     /locus_tag="VFB37_12715"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZO23357.1"
                     /translation="MKLNRTLGLLIVAASVGIGAAVAAAPPASTTTTTATSTPTAGKH
                     WHHHRGSMLVGTMLRATHQLNLTAEQQQTIKGILSSARAQHEKAGAASLDMTVLANPG
                     DPNYATALQTAKSRLATRLQNEIELQGQIYNVLTAEQKAQLPQVLAGMKAKAQERRAA
                     WQQQQAAGSN"
     gene            complement(5098..5709)
                     /locus_tag="VFB37_12720"
     CDS             complement(5098..5709)
                     /locus_tag="VFB37_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23358.1"
                     /translation="MRRGKAQICGLWALGALASMLHGAQAQDAAATPSSAGTPVASAG
                     EDSARPAPAADSSPSGTSTASAGPPVVCFKFTLQCFGNNSSQQSDGHSSQRAAASERT
                     AGANASSNTKDSSSTRGETAASATAAPGSLNLTPPDVRTIVSPQELQEPLPTQEQQAQ
                     ESESATVEVQSDPDTPDVPGGFGALWWALRHPTQAWRIFTPVE"
     gene            5959..6588
                     /locus_tag="VFB37_12725"
     CDS             5959..6588
                     /locus_tag="VFB37_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23359.1"
                     /translation="MQSFSQALQTQRWDDHRYYHHSRINQSLHLLSAVSFLAAYVLIF
                     TYPVAAALVGWLVAMVSRQLGHFFFEPKGYDTVNQATHEHKEAIKVGYNLRRKLVLLS
                     IWALAPVLLGLDPTLFGLFPAASGGFVQHVGQLWLAIGLGGLLFRTVHLFFLKDVQTG
                     LVWLTKILTDPFHDIKLYHRAPLHLLRGELIAPMTSASGLEASLSVHDA"
     gene            complement(6482..8206)
                     /locus_tag="VFB37_12730"
     CDS             complement(6482..8206)
                     /locus_tag="VFB37_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO23360.1"
                     /translation="MNPTSLAFFAAALAVGLAVIAAARRLQLRLELSRAKHRSLAGHA
                     RIARRLAALIPFYDYAASEFFAVDSAPPAIASRRQAGFMRLVALFAQRYPKTVEATAE
                     IQGALSDLQFTSRYRVPFQFSRLVRQHLRLGSVLQSSDGPVVTDLDGNRHYDLTGSYG
                     VNVFGHEFYKDCIEQGSQRVRGLGPVLGAYHPVVAENLRRLKEISGLDEVSFHMSGTE
                     AVMQAVRLARYHTRRPRLVRFCGAYHGWWGDVQPGVGNPLDARDTYTLRDMDERSLRV
                     LRKRRDIACVLVNPLQALHPNSNAPGDSALIDSARSAHFDRAAYASWLRKLREVCTAR
                     DVVLIFDEVFVGFRIALGGAQEYFGIRADMVTYGKTLGGGLPVGALCGRAGLMRRFRD
                     DRPADICFARGTFNSHPYVMGAMFEFLQRLQSGPIGACYAGLDELWNNRAQLLNERLG
                     AAALPVRVSNLSSIWTVCYLRPSRYNWMFQYYLRAEGLALSWVGTGRFIFSIDYSERD
                     FAEVAARIVAAASAMEADGWWWSDAALSNKSIRRRVLTEMLRARWRSSSARSAPRAAD
                     AAALGDRV"
     gene            8254..9117
                     /gene="asd"
                     /locus_tag="VFB37_12735"
     CDS             8254..9117
                     /gene="asd"
                     /locus_tag="VFB37_12735"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HZO23361.1"
                     /translation="MTTRTVIARIVRHENLNFLLTNRIPRRWLTLLVGRLSRVRQPLV
                     RAACLALWRLCCDLDLSDARKTHFDSLHDCFTRELKDGARPIDTDSSVLTSPCDGIIG
                     ACGAVAEGMVLQAKGFPYPVVDLLGDTELAAHYRDGSYVTLRLTSSMYHRFHAPYDCT
                     VEQVTYISGDTWNVNPIALRRIERLFCKNERAAIRCRLAQTDQLITLVPVAAILVASI
                     RLRFLEVPLHLRYRGPNRIGCQAKLLKGEQMGWFEHGSTLIVFAPRGVTLCEQWHGGN
                     VIRVGQRLFRI"
     gene            9350..12463
                     /locus_tag="VFB37_12740"
     CDS             9350..12463
                     /locus_tag="VFB37_12740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO23362.1"
                     /translation="MWAFRKSLRRFTIAAALLAAPCACQGAERWAGLADAVFQELAHD
                     NELPNAALPTSVAQDRDGFLWIGSQNGLARWDGYHFRDYRSDPGKAGSLPDNFIQTLF
                     SDVHGVLWIGTISGGLARYDRERDTFVSYPAGPTGLSSVSVRAIADDGTGGVWVGTDN
                     GLDHMVPGMASLSHLRHIEDDPRSLPDNRIRGLLHDREGTLWVGTASGLARLDSLATG
                     FVPVPLPAPEGKLPAAWNFLEDSAGHIWIGTVRHGAYIIESRVSGVARAVQESDGAHS
                     TLQDEGVGALIEPVAGQVWLGTVGQGIVAVDLATMHTRRIRHDPTLASSLQEDTIQAF
                     YKDRSGLIWICGNRSVSRFDAGQSAVLTVFGASRRPDSISDTNVDAVLPRPDGRVWVG
                     LDSNGIDILDPAGARAGALRPDPAHPGTALPKDFINAFAAADNGDVYIGTEQGLYRSD
                     PEGHRAVRVAVPLRDPVAPVWTLLMDENVLWLGGSDGLWPLDLSGGKLAPTTHADLTN
                     SLTDQRITSLARTGRFLWVGTKKGLNRLDLISHAVERILPVATDPSALSASYITSLLA
                     DSRGRLWVATFGAGVDILESWDANGRPRFRRLGIKQGFVNENANKLLEDLQHNVWVST
                     DDGLVRINESTFAIRTLRRAEGLPISSYWVGSGAVTQQGELLFGGVGGLTVVRPERLT
                     PWSYRPPIVITDIRIGGKKVAPGRLGVDDAAPALTIAPNANSVAVEFSALDYSAPERN
                     RYAYRLEGFDTAWVDSEPTRRLATYTNLPPGDYRLHLRGSNREGVWTEDTLSVPVRVL
                     PAWYQTMTFRLLVALLGIGVIVALVQIRTMYLKRSQRDLERQVVERTAELRESQRQLE
                     QIAYYDTLTALPNRRMFTEEFRELIVLARLQTGRFALLLIDLDRFKQINDSRGHDAGD
                     ALLIEAAIRLQAAVRKSDCVARLGGDEFAVLVAHNPAATDIEHICHRIIESFIMPVPV
                     NGAGLKSSASIGVAVFPDHGSTLDNLYKSADLALYEAKRAGGNQWRWFGAQTASIDTA
                     QNALGML"
     gene            complement(12495..12644)
                     /locus_tag="VFB37_12745"
     CDS             complement(12495..12644)
                     /locus_tag="VFB37_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HZO23363.1"
                     /translation="MTMTVSMGPVIALIAGILILIIPRLLNYIVAVYLILIGLLGLFG
                     HTAHL"
     gene            complement(12771..13943)
                     /locus_tag="VFB37_12750"
     CDS             complement(12771..13943)
                     /locus_tag="VFB37_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZO23364.1"
                     /translation="MRFSPLVERISGEGADAWLTHYEASAARERGEDVIILSVGDPDL
                     ETPQPVIERAIERIRAGDTHYTPAPGRDAVREAIAAAHMRRTGQATRAENVVFLSGAQ
                     NALFAASMLLAGPGDEVITFDPMYPTYPATLQASGARLLRAPTRADFRPDLDSLIALV
                     SSRTRAIAWATPNNPTGVVLSEAELAVIADLAHRFDLWLISDEVYAGLAPEGRVPSLA
                     ASLPERVVTLGSLSKSHAMTGWRAGWLVGPPELARQAEALAMCMLFGLPGFIQEAALV
                     ALSIAPEAEGRMRTYCSTRCERLRAGVVGVPGIRAHVPQAGMFMLLDVRDTGLSGYGF
                     MRSLYQAERVCVMDGAAFGGHTEGFVRVCFATSEAEIDEACRRIRRFCSSGPHAAP"
     gene            complement(13953..>14229)
                     /locus_tag="VFB37_12755"
     CDS             complement(13953..>14229)
                     /locus_tag="VFB37_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23365.1"
                     /translation="APPNARPEDIVGVTLDLQRGDMIAAPPITIGTLKIQHVSVTFAD
                     TFLFEHLKLNSKPTLLLGMDVLGSFDAIIIDYRLRELEILTRNAPES"
BASE COUNT         2524 a         4585 c         4577 g         2543 t
ORIGIN      
        1 aactggaggt caacccgaac cagctgcgct gggacccgct gccggtgccc gaggaaccca
       61 ccgacttcat cagcggcctg cgcacagctg gaggcaacgg ctccccgcag gcgcaatccg
      121 ggtgcggcat ccactggtat gtcgcgaatc gctccatgta cgagcgcttc ttctacgacg
      181 ccgatggcga gctcctcatc gtgccgcagc tcgggagact gcgcctggca accgagctcg
      241 ggctgctgca gatcgagccg cagcaggtcg cagtcattcc gcggggtctg cgctttcggg
      301 tggagctgcc cgacggtcag gcgcgcggtt acgtgtgcga gaacttcggc gcgccgttcc
      361 ggcttcccga cctgggtccg ataggctcga acgggttggc gaatccccgc gattttcaga
      421 cgccggtcgc ctggtacgag gatcgggttg gcgagctcga gctgatcgcg aaattccagg
      481 gccattgctg gtccgctccg atcgatcatt cacccctgga cgtcgtggcc tggcatggca
      541 atcatgtgcc ctatcagtac gacctgcggc atttcaacac gctgggctcg gtcagcttcg
      601 atcatccgga tccctccatc tatctcgtgc tgcagtcgct cagcgacact cccggagtgg
      661 atgcgatcga tttcgtcatc tttcccccgc gctggctggc cgcagaaagc acattccggc
      721 cgccctggta ccaccgcaat gttgcaagcg agttcatggg cctgatcact ggtgtgtacg
      781 atgccaagtc agaggggttc gcccccggcg gcgcatcgct gcacaactgc atgagcggtc
      841 acggaccgga tgcggccacc ttcgaacagg cgacccgtgc cgatacctca cggccccagc
      901 gcatcgacaa cacgatggcg ttcatgttcg agacccgcag cgtgatccgc ccgacccggt
      961 tggccctgga atcgccgcaa ctgcagagcg actacgcgcg ctgttggcag ggcttgcgca
     1021 gtcatttcac gccttcttcc cgctgaggac tgtgtgccgc tcaatgccac gcacgatcct
     1081 gcgctgaaga gctggctgga gtcggccaac gatgcgcagg ccgacttccc catccagaac
     1141 ctgccgtttg cggtgtttcg ccggcgtggc aggtcgcaag cgtggcgcgg tgggatcgcg
     1201 atcggcgatt gcgtgctcga tcttgctgag ctgcacacgc tgcgcctggt gcagggaacc
     1261 gccgccgatg cgctcgcggc gtgtaccggc tccacgctca atggcttcat ggctctgggt
     1321 ggcgaggcgt ggtcggcgct tcgagcagct gcctcagagc tgcttcgtac cggcgcggat
     1381 caagccagca cggtgcgggc agcgctggtg ccccagtcag aagttgaata cacgctgccg
     1441 gcctgcatcg gcgacttcac cgacttctac agctccatac atcacgcgac tcgcgtgggc
     1501 aggttgttcc ggcccgacaa cccgctcctg cccaactaca agtgggtgcc tattgcctac
     1561 cacggccgaa cctcttcgat aggcgtgtcc ggccagtgct tttcccgccc ggcaggacag
     1621 ctcatgccgt ccggcgcatc acagccgcag ctgctgccca cgcggcgcct ggattacgag
     1681 ctcgagctcg ggatactgat aggaccaggg aaccgcgccg gctgtccgat acccatcgag
     1741 gatgccgaga aacacatctt cggtctgtgc ctgctcaatg actggtcggc acgggatatc
     1801 caggcatggg aataccagcc cctgggccct tttttggcga agaacttcgc caccacgctg
     1861 tctccctgga tcgtgacgct cgaggctctc gagccgttcc gcgtgccttg gacacgcagt
     1921 gcgcaagacc cgcaacccct tccgtatctt gatgcgcggg agctgcgcaa tcgcggagcg
     1981 gtcgacatcc agctcgaggc gtacctcgag tcagagcaga tgcgagcggc aggcgcaccc
     2041 gcgcaccgac tttcgcactc cacattccag agcgcctact ggactgtggc gcagatgctg
     2101 gcgcaccaca cgatcaacgg gtgcaacctg cggacaggag atcttctcgg aaccggcact
     2161 caatcgggcc ctgaaggggg ccagggtggc tcactgctgg agctgactgc aggcggtcag
     2221 cagcccgtgg tgctcgcctc cggagagacc cgcaccttca tagccgatgg cgaccgggtg
     2281 agctttcgcg gctggtgcga aaagcccggc tgcgtacgga tcgggctggg cgaggtggcc
     2341 gccaccgtac tgccggcaaa tcagaaggcg aaatagggcc ccataccgcc ggcggtgttg
     2401 cggttctcga acgcagtgaa tacgtggcgc ccgaagtaga acggcaatcc ccagtcgaat
     2461 gactgggtgg cattgaagga gctgttcgag atcccggccc cgcccagatc gctggctgct
     2521 gcaatgacga tgctctgatt gaagagactg tcagcattcg cgatcgagaa attaaccggc
     2581 gagctcgtat tgttctgccc cgtgatcgtg gcgctgagac tcagcgtcga gctcgggcag
     2641 tagaatggat cctgctgcgg gtctgggcag cccggaatgc tgctgtcggt gaagaagaag
     2701 ccgttggatc cgctatcgat gaagctctca ggcagagcct gtcccttgta ttcggtcgag
     2761 aggctgccgt ccgaggcgag tgtgaatacc gcggcgctac ccagtccgtt gttgctctgc
     2821 gtgccgatcc caaacaccag cgagccgctc accgatgccg cgcccgttgc acccaccgag
     2881 ggcagctcga tgatcacacc gttattgtcg gtggcaaaaa acgaggccgg gttggaaacc
     2941 tgcgctgtga catccacggc cgtgttctgg caggaactgc tggaggtaca ggcgtagtag
     3001 atgggattgg gcacgatcga gcagcctgat ccacaatcgg aaatgaacgg tcccacgccg
     3061 aggatgccgt tggctccgaa agccgcgacc gtgtcttcgg ctttcccgac actcgaacag
     3121 gccgagggcg cgctcgtgtc ggggtaggcg gcatcgccga tgacctgcac ggcgattcct
     3181 gcggcagact ccccggcaat ctgcaggtcc gccgtccgga gggggcccca gctgaagccg
     3241 tcgacgaact gagtgcactc cacggttgca gcgccactgg catcggtatg caggggcaga
     3301 ttgagcgtca gcaccgaccc cagaatcctc agtcccgagg aaccggtatc gacctggatg
     3361 tgatcgatcg tctggcaact cgtcgtgctg cccggcacgc agaccgtaac cgtgacgaag
     3421 agggtattga cgtccggggt gttggacagg gcagtcgggc cgccgtccac gatcactgcc
     3481 tgcacgttgg cggcgggacc ggtgggctgg gtcgactgcg agccgaggcc ggatccacca
     3541 ccgccgcctc cgcagccggc cgagagcagc gccccgatga gggccgttgc aatgattgcg
     3601 cgcactcttg cgcctccgtt aattgatctt ggcgaccgag aaattcggcg gcaacagcga
     3661 ggggacccag gcttcgccaa aaaaggcgcg catgtggcca tgcgagtgca ccacgagatc
     3721 cggctgctcg atgcgcagcc gccggtgacc ggcgtgcggt gcgcttgccg cttcctgata
     3781 ctgcgcgaag tagctgccca gggtttgacg caagtcagga agcagcggcc cctgccacgt
     3841 gacggcgaag acgcgccccg cggcagacac gtactccttc accacggtgt tgccgggtgt
     3901 gcggattgcg tggatcgtgt agccttctgc ggcgctcgtc acctgtagcc caccgcgcat
     3961 ctgctggcga tcttgctcaa ccgagctcac atcctcaccc agggccgcca gcgccggctt
     4021 ccaggccggg atcgacagga tcccagcagt cacagcgacc catacggaca ggctcgtgcg
     4081 ccttgcgcgt ttcacgttga tagacccttt gaattccggg gaaacccagg acagccggct
     4141 cagcttgatc ccggcagtac ggcaggctca tacccgcctg agcgatggta gcggcaaatg
     4201 cccccgggtc aatgcggacc cgggggccgg tcctggaatc gagcgatcag tcgcactttc
     4261 cggctgctca attgctgccg gcggcctgct gctgctgcca ggccgcgcgt cgttcctgcg
     4321 ccttggcctt cataccggcg agcacctgcg gaagctgcgc tttctgctcc gcggtcagca
     4381 cattgtagat ctgcccttgc agctcgatct cgttctgcag ccgcgtagcg aggcgcgatt
     4441 tggccgtctg cagcgccgtg gcgtaattgg gatcgccggg gttggccagt acggtcatgt
     4501 ccagcgaggc ggcgccagcc ttctcatgct gcgctcgcgc ggagctgagg attcccttga
     4561 tcgtctgctg ctgttcggca gtcagattga gctgatgggt ggcacggagc atcgtgccca
     4621 cgagcatcga ccctcgatga tgatgccaat gcttgcccgc cgtgggggtg ctcgtggccg
     4681 tcgtggtagt cgttgacgcc ggcggcgccg cggccaccgc tgcgccgatc ccgacgctcg
     4741 cagccactat cagcagtccc aatgtcctat tcaacttcat ggcttcttcc tcaaaagtta
     4801 tagagcaatt gtaactatct ttgcagtgaa tctcgtgcgc cttccgccgg caggcgcagt
     4861 gtcggtgggc tgtcccgaga tcacgtgagt tgcagatgat tcactcatgg gaagcacagc
     4921 gctcaactgt accggccgtt gacagcccga ctgtaagcat ctgtgcgcaa atgtgtaacg
     4981 ggggaggagt gtggcatcgg gctcgatgca ggccccgcac ctgaacggca gctgattgga
     5041 atgcatcggg ccgccgtgcc cggcgtcatc agcggcggcg ctcgatccgt gcgccgatca
     5101 ctcgactggg gtgaagattc tccaggcctg cgtgggatgt ctcagtgccc accacagcgc
     5161 cccgaaaccg ccggggacgt ccggagtatc ggggtcactc tgcacttcga cggtcgcgga
     5221 ctcggactcc tgcgcctgtt gctcctgtgt cggcagcggc tcctgcaact cctgcggaga
     5281 cacaatcgtg cggacatcgg gcggggtgag attcagtgag cccggagcgg cagtcgcact
     5341 ggccgcagtt tcccctctgg tggaggagga gtctttcgtg ttggaagagg cgttcgcgcc
     5401 cgcggtccgc tccgaagcgg ccgcacgctg cgagctgtgc ccatcactct gctgtgatga
     5461 gttgttgccg aagcactgca gggtgaactt gaagcacacc acgggcggcc cggcgctggc
     5521 cgtggacgta ccgcttgggc tcgagtcggc tgccggagcc ggtcgagcgg aatcctcgcc
     5581 agccgacgcc acgggggtgc ccgccgaaga aggggtagct gccgcatcct gggcctgcgc
     5641 gccatgcagc atcgaagcca gcgcgccgag cgcccagaga ccgcaaatct gcgcttttcc
     5701 gcgcctcaag tgaacctgcc atgccgcgcc tcgagcgcgg cctttgtgtc aaaaggctac
     5761 tcctgtccgc cactgaataa cagcaaccgc tatgcccgca tttcgcggcc cgggaggcct
     5821 tcccagggcc ctagcgaggg ttccgcgccc ggcaggggcc tttcaggggc ctgcggatgc
     5881 aggctgtaac gtcgttgcaa cagcgttgcg atatttcatt cacggtcgac accgcactgc
     5941 cacagttcag gaaacaccat gcaaagtttt tcgcaagctc tgcaaactca acgctgggat
     6001 gaccatcgct actaccacca cagccggatc aatcagtcgc tgcatctgct gagcgcagtg
     6061 agcttcctcg ccgcgtatgt cctgatattc acttaccccg ttgcggccgc cctggtgggc
     6121 tggctcgtgg ccatggtgag caggcagctc ggacatttct tcttcgagcc gaagggctac
     6181 gacacggtca accaggcgac gcacgagcac aaggaggcga tcaaggtcgg gtacaacttg
     6241 cgccgcaagc tcgtgctgct gtcgatctgg gctctcgcgc cggtgctgct ggggctggat
     6301 ccgacactat tcggcctgtt tcccgccgca agcgggggat tcgtgcagca cgtgggccag
     6361 ctatggctgg cgatcggcct cggcggcctc ctgttccgca ccgtgcacct gttcttcctc
     6421 aaggacgtgc agacgggtct ggtctggctg accaagatcc tcacggaccc gtttcatgac
     6481 atcaaactct atcaccgagc gccgctgcat ctgctgcgcg gggagctgat cgcgccgatg
     6541 acgagcgcca gcgggctcga agcatctctg tcagtacacg acgcctgatg gacttgttgc
     6601 tgagggctgc atcggaccac caccagccat cggcctccat ggccgaggcc gccgcgacga
     6661 ttcgtgcggc gacctcggcg aaatcgcgct cggagtagtc gatgctgaag atgaagcgcc
     6721 cggtgcccac ccagctcagg gccagacctt cggcccgcag atagtattgg aacatccagt
     6781 tgtagcgcga tggccgcagg tagcagaccg tccagatgga agagagattg ctcacccgga
     6841 cgggcaatgc ggcggcaccc agacgctcgt tgagcagctg cgcccggttg ttccacagct
     6901 catccaggcc cgcgtagcag gcaccgatcg gcccgctctg caggcgctgc aggaactcaa
     6961 acatggcgcc catcacgtag ggatgcgagt tgaatgtccc tcgggcgaag cagatatcgg
     7021 ccggccggtc gtcgcgaaag cggcgcatca gcccggcacg cccgcacaaa gcgcccacgg
     7081 gcaggccgcc cccgagcgtc ttgccatagg tcaccatgtc ggcgcgaatg ccaaagtact
     7141 cctgagcacc gccgagagcg atgcgaaatc cgacgaacac ctcatcaaat atcagcacga
     7201 cgtcacgcgc ggtgcagact tcgcgcagct tgcgcagcca gctcgcgtaa gccgcacgat
     7261 cgaagtgtgc gctgcgcgcg ctgtcgatca gcgcagagtc tcccggagca ttcgagttag
     7321 ggtgcagcgc ctgcaatggg ttgacgagca cgcacgcgat gtcgcggcgc ttgcgcagca
     7381 cgcggagcga gcgctcatcc atgtcccgaa gcgtgtacgt gtcgcgcgca tcgagcgggt
     7441 tgcccacacc cggctgaaca tctccccacc agccgtgata cgcaccgcag aagcgcacga
     7501 ggcgcgggcg cctggtgtga taacgcgcca gtcggaccgc ctgcatgacg gcttccgtgc
     7561 cggacatgtg gaaggagacc tcgtcgagac cggagatttc cttgagtcgc ctgaggttct
     7621 ctgcgaccac agggtgatag gcgccgagga ccggaccgag ccctcgcacc cgctggctgc
     7681 cctgctcgat gcaatccttg tagaactcgt gtccgaacac gttcacaccg tacgagccgg
     7741 tgagatcgta gtgccgattg ccatcgagat cggttactac cggaccatcg gaggactgca
     7801 gcacggaacc gagcctgaga tgctgtcgga ccagccggct gaactggaag ggcacgcggt
     7861 agcgggacgt gaactgcagg tcggacagcg ctccctggat ttcggcggtg gcctccaccg
     7921 ttttgggata gcgctgtgca aagagcgcta ccaggcgcat gaagccagcc tggcgccggc
     7981 ttgcgatcgc gggtggcgcc gagtcgactg cgaagaattc cgaggcggcg tagtcgtaaa
     8041 acggaatcag ggccgcgagt cggcgcgcaa tccgtgcgtg gccggcaaga gagcgatgct
     8101 tggcgcgcga gagctcgagg cgcagctgca acctgcgcgc cgcggcaatc acagccaggc
     8161 ccacggcgag cgcagcggcg aaaaaggcga gcgatgtcgg gttcatggga ctcctcagac
     8221 cggcaaattt tattgcagtt gagtgacagc cagatgacca cccgaaccgt gatcgcccgc
     8281 atcgtccggc atgagaatct gaacttcctg ctgaccaacc ggatcccccg ccgctggctc
     8341 actctgctgg tcgggcgtct gagtcgcgtg agacagccgc tggtgcgcgc tgcctgcctc
     8401 gcgctgtggc ggctgtgctg tgacctggat ctcagcgatg cccgcaagac acacttcgac
     8461 agcctgcacg actgcttcac acgcgagctc aaggatggag cacgtccaat cgacaccgac
     8521 agcagcgtgc tgaccagccc ctgcgatggg atcatcggcg cctgcggcgc ggtcgcggag
     8581 ggcatggtgc tgcaggcgaa gggatttccg tatccggtgg tggacctgct cggcgatacg
     8641 gagctcgccg ctcattatcg cgacggctcc tacgtgaccc tgcgactgac ctcgagcatg
     8701 tatcaccgct ttcacgcacc ctacgactgc acggtcgagc aggtgaccta catctcgggg
     8761 gatacctgga atgtgaaccc gatagcgctg cggcgcatcg agcggctctt ctgcaagaac
     8821 gagcgggcgg cgatccgctg ccggcttgct cagactgatc agctcatcac actcgtgccg
     8881 gtggcagcca tcctcgtggc gagcatccgc ttgcgcttcc tcgaggtgcc gctgcacctg
     8941 cgctaccgcg ggccgaaccg catcggctgc caggcgaagc tgctcaaagg cgaacagatg
     9001 ggctggttcg agcatggatc gactctcatc gtgttcgcgc ccaggggtgt gacgctgtgc
     9061 gagcagtggc atggcggaaa cgtgattcgc gtcggccagc ggctgttcag gatctgatcc
     9121 agaaggcact gcgcgggggg tccgtcagcg aagttgcgac ccgcgtcaaa ttcgggccgc
     9181 aagcgagctg gagggcggtt tctgccacgc cgttggccga ctgcagcggc tggcgccggg
     9241 gagcggcggc gcgcgtgatg cagggcacat cttccgggtt tctttgtcgg ccgcacggga
     9301 tcgccggtag tagtatcggt gtggcgtcac tcccctgatt ttctcctcga tgtgggcatt
     9361 cagaaagtct ctccgccgat tcacgatcgc cgcggcattg ctggcagcgc cctgcgcctg
     9421 ccagggcgcc gagcgctggg cgggactggc tgacgcagtt tttcaggagc tcgcgcatga
     9481 caatgagctt cccaacgctg cgcttcccac ctccgtagcc caggaccgtg atggcttcct
     9541 gtggatcggc tcgcagaacg gactcgcgcg ctgggacggt tatcatttcc gcgactaccg
     9601 ttccgatccc ggcaaagccg gctcgctgcc ggacaacttc atccagactc tcttcagtga
     9661 cgtgcacggc gtgctctgga tcggaacgat ctcgggcggt ttggcgcgct atgaccgcga
     9721 gcgcgatacc ttcgtctctt acccggcagg ccctactgga ctgagcagcg tcagtgtccg
     9781 ggccattgcc gatgacggca ctggcggcgt ctgggtggga accgataacg ggctcgatca
     9841 catggttccc gggatggcga gcctctctca cctgcgccac atcgaggacg atccgcgcag
     9901 tctgccggac aaccgaattc gcggcctgct gcatgaccgg gaaggtacgc tctgggtagg
     9961 taccgccagc ggcctcgccc ggctcgatag ccttgcgacc ggcttcgttc ccgtgccgct
    10021 gcccgcaccg gaaggcaagc tgccggcggc ctggaatttc ctcgaggaca gcgccggcca
    10081 catctggatc gggaccgtgc gccacggcgc ctacatcatc gagtcgcgcg tgagcggcgt
    10141 cgcgcgtgcg gtccaggaaa gcgatggcgc acattcgaca ctgcaggatg agggcgtagg
    10201 ggcactcatc gagcccgtcg ccgggcaggt ctggctcggt acggtggggc agggcatcgt
    10261 agcggtggat ctcgcgacga tgcatacgcg acgcatccgt cacgatccga ccctggcatc
    10321 gagtctccag gaggatacga tccaggcttt ctacaaggac cgctcggggc tcatctggat
    10381 ctgcggcaat cgcagtgtca gccgtttcga tgcggggcag tcagctgtcc tgacagtctt
    10441 cggtgcctcc aggcgtccgg acagcatctc cgacaccaac gtcgatgcgg tgctgccgcg
    10501 gcccgacggt cgggtctggg tgggcctgga cagcaatggc atcgacatcc tggatccggc
    10561 cggcgcgcgc gccggagcgt tacggcccga tccggctcat cccggaacag cgctgccgaa
    10621 agacttcatc aacgccttcg ctgctgctga caacggggac gtctacatcg gcaccgagca
    10681 gggcttgtat cgaagtgatc cggaaggcca tcgggccgtc cgggttgccg tgccgctgcg
    10741 tgacccggtt gcccccgtgt ggacgttact gatggatgag aacgtgctgt ggctcggcgg
    10801 ctccgacggg ctctggcctc tcgacctctc ggggggcaag cttgccccca ccacgcatgc
    10861 agacctcacg aacagcctca ccgaccagcg catcacctcg ctcgcacgca ccggccgctt
    10921 cctgtgggta ggtacgaaga aaggcctcaa tcgcctcgat ctgatcagcc atgccgtcga
    10981 gcgcattctg ccggtggcaa cggatccaag cgcgctgagc gcaagctaca tcacctcgct
    11041 gctcgcagat tcgcgtggcc gcctctgggt cgcgaccttc ggcgccggag tggacattct
    11101 cgaatcgtgg gacgcgaacg gccgaccccg tttccggcgc ctgggaatca agcagggctt
    11161 cgtcaatgag aatgccaaca agctgctgga ggatctgcag cacaacgtct gggtgagcac
    11221 cgacgacggt ctcgtgcgca tcaacgagag cacgttcgcg attcgcactc tgcggcgcgc
    11281 cgagggcctg cccatctcca gctactgggt cggctccggc gcagtcacgc agcaaggcga
    11341 gcttcttttc ggtggcgtgg gcggcctcac cgtcgtgcgc ccggagcggc tgacgccctg
    11401 gagctatcgc ccacccatcg tgatcaccga tatccgcatc ggcggcaaga aagtcgcccc
    11461 ggggagactc ggcgtggatg atgccgcccc cgctctcacc atcgctccga acgccaacag
    11521 tgtcgcagtg gaattctcag cgctcgacta ctccgctccc gagcgcaatc gctatgcgta
    11581 tcgtttggag ggtttcgaca ccgcctgggt cgattccgag cccacgcgac gtctggcgac
    11641 ctacaccaac ctgccgcccg gagactaccg gctgcacctg agaggctcca accgcgaggg
    11701 cgtgtggacc gaagacaccc tgagcgtgcc ggtccgggta ttgccggcct ggtaccagac
    11761 gatgaccttc cgcctgctgg tggcactgct cgggatcggt gtcattgtgg cgctggtgca
    11821 gatccgcacg atgtacctca agcgcagcca gagggacctc gagaggcagg tggtcgagcg
    11881 caccgcggag ctgcgcgaga gccagcgtca gctcgagcag atcgcctact acgacacgct
    11941 cacggcactt ccgaatcgca gaatgttcac ggaagagttt cgtgagctga tcgtacttgc
    12001 gcgcctgcag acggggcgct ttgcgctgct gctgatcgac ctggatcgct tcaagcagat
    12061 caacgattca cgcggccacg acgctggcga tgcgctgctg atcgaagctg cgatccgcct
    12121 gcaggctgcg gtccgcaaat ccgactgcgt tgcgagactc ggcggtgatg agtttgcggt
    12181 actggtcgcc cacaatcccg cggccactga catcgagcat atctgtcacc gcatcatcga
    12241 aagcttcatc atgccagtcc cggtgaatgg cgccggtctc aagagcagtg ccagcatcgg
    12301 cgtcgcggtg tttccggatc acggctcgac gctcgacaac ctgtataagt cggcggacct
    12361 ggccttgtac gaagccaagc gcgccggcgg caaccagtgg cgctggtttg gcgctcagac
    12421 cgccagcatc gatactgcgc agaatgctct cggcatgctg tgaccggccg ttggcccgtc
    12481 acagtgccgc agcgctacag gtgcgctgtg tggccgaaaa gtccgagcag accgatcagg
    12541 atcaggtaga ccgcgacgat atagttgagc agccgtggga tgatgagaat gagaatgccg
    12601 gcgatcaggg cgatcaccgg gcccatggaa acggtcatcg tcatggaagt ggcctcattg
    12661 ttcagtgaac actgtgcgct ctccggcagg ccgcgcaccg caccagccgc tgaaacgggt
    12721 actacacgag cgcaagagat ctacgctcga gggccctccc ggttccgggg ttagggcgca
    12781 gcgtgcggtc cgctcgagca gaagcggcgg atgcgccggc aggcctcatc gatctccgcc
    12841 tcgctggtcg caaagcacac gcgcacgaac ccttcggtat ggcctccgaa ggcggcccca
    12901 tccatgacgc agacgcgctc ggcttgatac agcgaacgca tgaacccgta gcccgagaga
    12961 ccggtatcgc gtacgtcgag cagcatgaac atgccggctt ggggcacgtg cgcccggatg
    13021 ccaggcacac cgacaacccc cgcccgaagg cgctcgcacc gcgtgctgca gtaggtgcgc
    13081 atccgtccct cggcctccgg tgcaatggac agcgccacga gggcggcctc ctggatgaag
    13141 ccaggcaggc cgaacagcat gcacatcgcc agagcttccg cctgacgcgc gagctcgggc
    13201 ggtccgacga gccatcctgc gcgccagcct gtcatggcat gcgatttcga gaggctgccc
    13261 agggtgacga cccgttcggg caggctcgcg gccaggctcg gcactcgacc ttcaggggcc
    13321 agccccgcat agacctcatc ggagatcagc cacaaatcga agcggtgcgc gagatctgcg
    13381 atgacggcga gttccgcctc gctgagcacc acgccggtcg ggttgttggg cgttgcccag
    13441 gcgatcgccc gggtgcggga ggaaacaagt gcaatcagcg agtccaggtc cggacgaaaa
    13501 tccgcgcgcg tcggtgcgcg tagtaagcgc gcaccggatg cctgcagggt ggcgggatac
    13561 gttggataca tcgggtcgaa agtgatgact tcgtcgcctg gtccggcaag aagcatcgag
    13621 gctgcgaaca gtgcgttttg cgccccggag aggaacacga cgttttcggc tcgagtcgcc
    13681 tgacccgtcc gtcgcatgtg ggcagcggcg atggcctcgc gcaccgcatc gcgtcccggt
    13741 gcaggcgtgt aatgcgtgtc accggcacga atgcgctcga tcgcgcgttc gatcaccggt
    13801 tgcggcgtct cgagatccgg atcgccgacg ctgagaataa tgacatcctc gccacgctcc
    13861 ctggccgcag aggcctcata gtgcgtcaac caggcatcgg cgccctcgcc gctgattctt
    13921 tcgacaagag gcgagaatct catgcgggac gatcacgact ccggcgcatt gcgcgtcagg
    13981 atctccagct cccgcagccg atagtcgatg atgatggcat cgaaagagcc gagcacatcc
    14041 atacccagca gcaacgtcgg cttgctgttg agtttcagat gctcgaacag gaacgtgtcc
    14101 gcgaacgtga cgctgacgtg ctggatcttc agggttccga tggtgatggg tggggctgcg
    14161 atcatatctc cccgctgaag gtcgagcgtg acaccgacaa tgtcctccgg ccgtgcgttg
    14221 ggtggcgct
//
LOCUS       DASRHN010000323        16310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2256544, whole genome shotgun sequence.
ACCESSION   DASRHN010000323 DASRHN010000000
VERSION     DASRHN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256544"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFB37_12760"
     CDS             <1..372
                     /locus_tag="VFB37_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HZO23366.1"
                     /translation="MEVKLKVHNRTDRTGRRAGARGVLGRMAVPAVAATMLLGAALPA
                     VSAVGIDIQIAPPAPRVVEVPPPRAGFVWAPGYYRWDGHQHVWVEGRWLRERRGFHWV
                     PEHWDERHGRYHFEPGHWVRG"
     gene            547..2205
                     /locus_tag="VFB37_12765"
     CDS             547..2205
                     /locus_tag="VFB37_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HZO23367.1"
                     /translation="MPRSTPFEAAPARVMPRLLVLALLVGPGPLLAQEAQRDAQNTQH
                     DKGPVCASVCLASDTAADFTQSPQSPQQESASPTAFAAPPPKPVLQWGTGDARSYWVP
                     AAEVPSWELLLNRFDHYVVDPGTYPSPITNFRANLHRSWVVDNDTFATNQFLHPYQGA
                     IYQGFARSAGLDFWQASAYAFAGSLIWEESGENTAPSINDQIATGIGANFFGEPLFRM
                     ASLLLETSPNESPSWWREVAAAIISPPTGFNRLVYGNRFKPVFRSYDPAVFTRVDVGA
                     NLLTHFSSNVNVNADPSAPPAGQTLNVGKEVASLSLAYGLPGKPGYDYDRPFDYFDFQ
                     LALDTSNAVESVFSRGLLYGTDYSAGPNYRGLWGLYGIYDYVAPNIFRISNTAAGLGT
                     TGQWWLSPAVAMQGSAVTGVGYAGGGVIHGSGVRPPGPLGDGQRNYHYGVTPEAILAL
                     RLLMGDRVALDTSARGYYISGVGATESRGSETINRVDVALTVRVFDLHGITLRYEVSH
                     RDGRYGTIEDSQQRVSTINLGYTLLGHARFGAVDWRPGSQARSD"
     gene            2490..3065
                     /locus_tag="VFB37_12770"
     CDS             2490..3065
                     /locus_tag="VFB37_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO23368.1"
                     /translation="MRAFSEEHFKHPAWGYSHCMRDYSLARQMAAADHAKVDDDVLFA
                     AAFLHDMAGFAPWQKKGVDHADQGALVVDTVLKGTGFPMGKIEGVRAAIRGHNYDRTP
                     VGPEALYLHDADALDWLGDIGVARIIAIVDPNGGQPDSHKAVAILEDNLAHVPEHVLS
                     AAGRALMPARRAELEQFLKTLRSETDDLRTL"
     gene            complement(3119..3376)
                     /locus_tag="VFB37_12775"
     CDS             complement(3119..3376)
                     /locus_tag="VFB37_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23369.1"
                     /translation="MKRELPIKGQKYIVAISPEGLKLTLKGRRNGVELKWEELVSGEA
                     AMAVALNASLGKLAGREPQARSQAPAAAPSKPAKPARKAAK"
     gene            complement(3444..5654)
                     /locus_tag="VFB37_12780"
     CDS             complement(3444..5654)
                     /locus_tag="VFB37_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO23370.1"
                     /translation="MPTVVVIGTTPLPGLGTPIQDVPANVQVYTSTDIARQGQTNLTD
                     FMEQNAGSITANAAQGNPFQPDINFRGFTASPLLGTPQGISVFQDGARINEPFGDIVN
                     WDLIPQSAIAGMQLIPGSNPAFGLNTLGGALALYTKSGTEYPGGGLQVSGGSFGRKTA
                     QAQWGGVAGSLDYFATGEFFDDRGWAQHNPSRVEQLFAKVGWQNEKTDLDLSFTGADN
                     RLQGTQTLPLSFLNDIQQAYTFPDLNINKLAFVTLKGSRFLSDSLLLGGNAYYRQYRN
                     QSVASDVNDDYGSIDPNTGAIDQVQATNDRSVIDQRSYGAGLQLVLTAGPRAHSNQLT
                     IGTSIDAGHARFTQDAQDAQFTANRNTVGIDDFALETDADSSSSYLGLFAADTFHLTP
                     KWTLTGSGRYNHADISISDRTGLSPLLNGDHSFSRFNPAVGLNYNPDPHLTGYLTYNE
                     GVRPPTAIELTCADANAPCKLPNDFLADPALRQIVSRTIEAGARGTWRSVWQWSAALY
                     RTQLDNDIEFISTAAGASTAGYFSNVGTTRRQGVELSLRTQIQSLSLATHYTYTDATF
                     RTPFSESSPSNSTADADGGIQVLPGDHIPGIPTHALKLRLDYAISSHVSVGTNVLVNS
                     SSYPRGDENNLDAGGKVPGYTLVNLDAQWRPADSLEIFARVSNLLDRRYSNFGILGEN
                     FFTGPNHTFGPTAGVDPRTEQFRGPGAPFGIWLGARYSMGGSGVSGSSGAGDRD"
     gene            complement(5868..7148)
                     /locus_tag="VFB37_12785"
     CDS             complement(5868..7148)
                     /locus_tag="VFB37_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZO23371.1"
                     /translation="MHVSLHETEHLLFVTLLQLIVMIGAARLMNTVLRALGQPGVIGE
                     ILAGLMLGPSLFGYFFPQASHALFGAKASVPITIISQLGLILLMFQIGMDFQFAHLRD
                     RRNTRTAILVAFSSITVPFLLGLLIGRLSAPHLAPGMDPVVYSLFFAVGLAITALPVL
                     GRILREYGLTRTEIGVIAISAAAVNDVAGWIMLAGISALAAARFSGSGMLLQMGGLAL
                     FGAASWLVLRPLAAWLVSRQRLREGEVPPNLMAIVIVLLFGMGICTYQLGVFASFGGF
                     VAGLMFHRFDSFVAAWQRQVGRFALVFFLPVFFSYTGLRTDVLGLTAWSDLQWLGLIL
                     AAAIAGKILPVYLGGRLCGFDRHRAWVLGSLMNTRGLVELIALNVGYDLGFIPQKVFT
                     MLVAMAVLTTLMTGPLLKVLLPRTGHAIPLAVEA"
     gene            7406..9295
                     /locus_tag="VFB37_12790"
     CDS             7406..9295
                     /locus_tag="VFB37_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing diguanylate
                     cyclase"
                     /protein_id="HZO23372.1"
                     /translation="MTVLAGVATPCIVGAASAVNPAVSFIEHGVIEMRTDPEASRRDA
                     EAALQSLQRQPDVDLEIQARLILCDYQAERDTRAALEQIEAATALLPQAQRKGIQAGL
                     LDCRGEIFETAGDNTRARGLYEQAVAVATSTHDEEMLAQSLFSRGYLLGLQGEYAAGL
                     ADLKRAQSLFDRLNKPLHSLTTLNSIAILYNRMGDYAEARSIYSRALTAQRSAGMKRE
                     LVVTLHNLGRASENLHDWDDARQSFSESLALSRQLGYSRGEAYALRGLAAVSNASGDP
                     KGALETLDRAMQLQQKTADARLLGQIQLARGVAYHKLQRLTESATALESALQIFLRAD
                     ALGELNATYSELADVYAESGNWRAAFERQTHAKLTSEKLLGNQLDQRFAALKVEFDTA
                     SKEKENAALMRENQANEKALAQGRAVRHLQAAVIALSAALVLVLALLAVYQRRSALRL
                     RHLAMTDELTGVPNRRSVLRRLEPLLKDSLKSCSALIIDIDHFKKINDQLGHPIGDEV
                     LKVVAARLRASVVEPAFFGRLGGEEFLIVLPATGLEEARMAAESFREEILTVDTAHWF
                     GEEARHITASVGCTVSTPGEDSPSTILKRADAALYAAKRSGRNCVRTEPAVQQEFPSL
                     LIGTQ"
     gene            9292..10632
                     /locus_tag="VFB37_12795"
     CDS             9292..10632
                     /locus_tag="VFB37_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO23373.1"
                     /translation="MTEPADRWGERLPRRIGLLNAIAVLIGVTVGSGIFRVPATVAAQ
                     LHDPGPVILCWILGGLVALFGALTLAELAGALPRSGGIFAYLLESFGPAPAFLFGWTE
                     LAVVRASALGATATIFAEYLGYFFPLSAAGVRRVAALAIIVIGALNYVGVRRAAAVMT
                     IATVAKYVAVMALGVLAFSASAAGGARFTPAWGTGLHPSLLVTALIPVMWTYDGWADL
                     SFMGGEVRNPQRTLPLALILGTICVMLVYLIINIGFMHLLPLPQMAQSKLIAATAAEH
                     IPLFGGSGAAVIAAVVLISTFSGLNGSMMTGPRIFFAMADRGLFFNAVARVSPRFDSP
                     SVAIWLATTLGVVYVLQNDFAQLADRFILGIWPFYALAVAAVFVLRRKRPDLERPYRV
                     LGYPAVPALFLLASLAMVVNAFITDPVNTGITFGIILAGIPAYYLWRLRKRVPG"
     gene            10714..13053
                     /locus_tag="VFB37_12800"
     CDS             10714..13053
                     /locus_tag="VFB37_12800"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HZO23374.1"
                     /translation="MDQFRRASLLALVTLTSAATVASATTETEAGGSPTAPIFGFTTA
                     QTSDERSLEARFDGDLNAADLDAWMKNLSAEANNVGAPHDKANAEFVRDQLKQWGWDA
                     QIEVFYPLYPTLKQHTLELVAPTRFVTTLKEPAVSGDATSSRTDGMGPYNVYGADGDV
                     TGDLVYANYGMPDDYKDLARRGIDVKGKIVITRYGAGWRGLKPKLAQEHGAIGCIIYS
                     DPHEDGYARGDVYPKGGWRPPEGVQRGSVADMPVYAGDPLTPGVGATKNAKRLPLSQA
                     KSLLKIPVIPISYGDAQPLLAALAGPVAPSSWRGSLPITYHMGPGPAKVHLAISSDWG
                     QKPLYDVIARIPGSQDPDEWVMRGNHRDGWVFGAWDPLSGHVAMMAEAKAIGALLKTG
                     WRPKRTLVYASWDGEEPGLLGSTEWAETHAEELQHKAVLYLNSDTNTRGFLRVGGSHS
                     LQHLADDVGAGVTDPEMHVSVLARLRAREMVEGYEKGASESEKQEAKLAAAGGDLPIE
                     ALGSGSDFTPFLQHLGIATLDVSYGGEDDQAGVYHSDYDSYDHYIRFGDPGFVYGVTE
                     AKTVGHLILRMADADVLPMQFGGFHDAVGRYVSELHELADHKRQAAQELGKLLDQNAF
                     ALATDPTRPVLAPQREPEVPYLDLAPLDNAVARLQKDAKQYDERYAGLLAGTLKLSAA
                     QSRQLDALLRGMEQTLLDSRGLPGRDWYKHLIYAPGLYTGYGVKTLPGVREAIEQNQW
                     EQANQYSAMTAAVLNAYCERLEQATQLLGPNKIEARQGS"
     gene            13050..14273
                     /locus_tag="VFB37_12805"
     CDS             13050..14273
                     /locus_tag="VFB37_12805"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZO23375.1"
                     /translation="MKRLLTTALLVGAATLMPLRLAVAARHDQTPAAEAAADARPLAH
                     PGAGPASDARFVGHVFLIVLENQSFDVTFGKRSPAPYLAKTLRDQGALLRNYYGIGHS
                     SLDNYIALVSGQAPNEQTQGDCPIVTEFKLSQPALDAHGQALGTGCVYPAIVMSLPDQ
                     LEAAGLTWKGYMEDMGNVPARESSTCGRSPLNSQEKSYVSVRGDKYAARHDPFIYFHR
                     IVDDAKRCNSHVVNLDRLPKDLQSIESTPNFTFISPNLCNDGHDAQCVDGKPGGFQAI
                     DSFLQAWVPRILDSPAYKQDGVLIITFDESDSAGPEASTACCGEKPLPGARQAPGFNG
                     PGGGRIGAVLLSAFIKPGTVSTVPYNHYSLLRWVEDQFGLAHLGYAGEAGLRTFGSDV
                     FTARADLPAKSSPSP"
     gene            14326..14808
                     /locus_tag="VFB37_12810"
     CDS             14326..14808
                     /locus_tag="VFB37_12810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO23376.1"
                     /translation="MDRVLQKILIREFRIGDEPALHDVFLSAVHELAAGDYTPEQIDA
                     WAPRFVDASLWADRMRGISPFIAEHDGVPIAYADVQSNGYIDHFFVSAPYARMGIGTR
                     LMRRIHATATSNGMRELTSNVSRTAQPFFKHFGFTVVEERQPIVRGIAIANAFMRKTL
                     "
     gene            complement(14814..>16310)
                     /locus_tag="VFB37_12815"
     CDS             complement(14814..>16310)
                     /locus_tag="VFB37_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO23377.1"
                     /translation="VGGFLFMKLPGSFLPEEDQGYGLIDLQLSPGASLQRTLDVLQQL
                     DGELKKNPAVSFTFLIAGSSFTGAGENVGRAFVHLKPWNERSTTAADFIAWAAATLPS
                     KLRDARISVFNLPTIRGLGQFGGFDFFLEDRAGLGREALTRAQDTVLREAGRDTTLTG
                     VRLNTLDPAPQLRLTVDRVQAQSMGLSVSDVYTAIQLMLAPVYADDFLYEGRVLRVLL
                     QADAAYRMSPEALGHFYVIGANNAMIPLSAVVRTDWLVASPSLTRYNGYPAVEITGSN
                     APGRSSGEAMQAMEQIVSRKLPHTIGYDWAGQSLQELLSGAQAPMLFALSILVVYLCL
                     AALYESWSVPAAVLMAVPVGLIGSTVAALLRGMPNDVFFKVGLITIIGLSAKNAILIV
                     EFAVTLRRAGKSLHEAVLEAARLRFRPILMTSFAFILGVFPMAVSSGAGANARRAIGT
                     AVVGGMLSAALLGVLLIPVCFVAVRRLMGDKLDEPSPQPMAPQPSGHS"
BASE COUNT         2899 a         5196 c         5356 g         2859 t
ORIGIN      
        1 atggaggtta aattgaaagt ccataaccgc accgatcgaa ccgggcgtcg cgcgggagcc
       61 cggggagtcc tggggcgcat ggctgtgccg gccgtggccg caacgatgct cctgggcgcc
      121 gcgttgcccg ccgtgtcggc cgtcgggatc gatatccaga ttgctccccc ggcgccgcgt
      181 gtcgtggaag tgccgccgcc gcgtgccggg ttcgtgtggg cacctggtta ctatcgctgg
      241 gacggccatc agcacgtgtg ggtcgaggga aggtggttga gagagcgtcg cggtttccac
      301 tgggtcccgg agcactggga cgagcgccac gggcgctacc acttcgagcc cggtcactgg
      361 gtccgcggct aggaagctca cagcgtgtgc gatggagagc gcggccgcgc tctccatcgc
      421 cacggatctg gatggagcga tgcgggccgc gaggcactcg tggctcctct gtaggtcaaa
      481 cgcctacgca cttgtaggcg aagcgccgat gttcatcggc cccccaagca ggcgacgctg
      541 cagcccatgc cgcgaagcac gcccttcgag gcggcaccgg cccgggtcat gccgcgcctg
      601 ctggtcctcg ctctcctcgt cggcccgggg ccgctcctgg cgcaggaggc gcaacgcgac
      661 gcgcagaaca cgcagcacga caaggggccc gtctgtgcgt ccgtttgtct ggcgagtgat
      721 accgctgcgg atttcacgca gagcccccag agccctcagc aggagagtgc gtccccgaca
      781 gcctttgccg cgccgccccc gaagcccgtg ctgcaatggg gtactggcga tgcccgcagc
      841 tactgggttc cggctgccga ggttccctcg tgggagctcc tgctgaaccg cttcgatcac
      901 tacgtcgtcg atcccggcac gtatccctcg ccgatcacca acttcagggc caatttgcac
      961 cgcagctggg tggtggacaa cgatacgttt gccacgaacc agtttctgca tccctaccag
     1021 ggcgcgatct accagggatt cgcgcggtcc gcgggcctgg acttctggca ggcctccgcc
     1081 tacgcctttg ccggcagcct catctgggaa gaaagcggcg agaacaccgc accctccatc
     1141 aacgatcaga tcgctactgg catcggcgca aatttcttcg gcgagccgct tttccggatg
     1201 gcgagcctgc tgctcgagac cagccccaac gagagtccgt cgtggtggcg cgaagtggct
     1261 gccgccatca tctctccgcc caccggcttc aatcgactcg tgtatggcaa ccgcttcaaa
     1321 cccgtattcc gcagctatga tcccgcggtc ttcacccgag tggacgtcgg cgccaacctc
     1381 cttacgcatt tctcctccaa cgtaaacgtc aatgccgatc cgagtgcgcc acctgccgga
     1441 caaactctga acgtcggcaa ggaggtggcg agcctgagcc tcgcctacgg cctgccgggc
     1501 aagcccgggt atgactatga ccgtccattc gattacttcg atttccagct cgccctcgat
     1561 acctccaatg cggtcgagtc ggttttctcg cgcggtctgc tgtatgggac cgactactcc
     1621 gccggaccga actatcgcgg cctgtggggg ctctacggga tctacgacta cgtggcgccc
     1681 aacatctttc ggatctccaa tactgctgcg gggctcggca ccaccggcca gtggtggctg
     1741 tcacctgcgg tcgccatgca gggaagcgca gtgaccgggg taggctatgc cggaggaggc
     1801 gtcatccacg gctcgggggt gaggccgccg ggaccgctag gggatggcca gcgcaactac
     1861 cactacgggg tgactcccga ggcgatcctc gcgctacgcc ttctcatggg cgatcgcgtg
     1921 gcgctcgaca cgagcgcacg cggttattac atcagcggcg tcggagcgac cgaaagccgc
     1981 ggcagcgaga cgatcaaccg ggtggacgtg gctctgaccg ttcgggtgtt cgacctgcac
     2041 ggcatcacgc tgcgctacga ggtttcgcac cgcgatggtc gatacggcac cattgaggac
     2101 tcccagcaac gcgtctcgac gataaacctc ggctacacgc tactcggcca cgcccgcttc
     2161 ggggcggtcg actggcgtcc gggcagccaa gcgcgctcgg actgagccct cccgccatga
     2221 cgaggcactc ggggagcgtt cccgtcgcac tgcccacctt caactcccat gcgagcgaat
     2281 gctaaggtgc gcccctcgca gccgttgcag ccccgggggg taatatgaag aacaaaacga
     2341 cacacacgcg ctggatctgt gagctactcg tcccctgcgc agcacttaca ctgctgatct
     2401 gcccgagcgc tccggggtgg gcgcaatcct ctgccggcga ggcccccaaa cgggcggccc
     2461 ctgggcccgc ggccggctgg cgtgagaccg tgcgtgcgtt ttcggaggag cacttcaaac
     2521 atccggcgtg gggctactcg cactgcatgc gcgattattc gctggcgcgc cagatggcag
     2581 cggccgatca cgcgaaggtg gatgacgatg tgctgttcgc tgccgcattc ctgcacgaca
     2641 tggccggatt cgcgccttgg cagaagaagg gagtcgatca cgcagaccag ggggctctgg
     2701 tcgtggacac cgtcctgaag gggacgggtt ttccgatggg taagatcgag ggggtgcgcg
     2761 ccgcaatccg gggacacaac tacgatcgga ctccagtggg tcctgaagcg ctgtacctgc
     2821 atgatgccga cgcgctcgac tggctgggag acatcggcgt ggccaggatc atcgccatcg
     2881 tggacccgaa tggcgggcag ccggatagtc acaaggcggt cgccattctc gaggacaacc
     2941 tcgcccatgt gcctgagcat gtgctctctg ccgccggccg tgcgttgatg cctgcacgac
     3001 gagcagagct cgagcagttc ttgaagacac tgcgcagtga gaccgacgat ctgcgcacgc
     3061 tgtagccgat cctgaacgct gaccgggtgc gggtggtggg gcgatatggg gtgcgggact
     3121 actttgctgc tttccgcgca ggttttgcgg gtttgctcgg agcggcggca ggagcttgcg
     3181 agcgggcttg gggctcgcgt cccgcgagct tgcccagtga ggcattgagc gcgacagcca
     3241 tggccgcctc gccgctcacc agctcttccc acttgagctc cacgccgttg cggcgcccct
     3301 tgagggtgag cttcagtccc tccggagaga tcgcgacgat atatttctgc cccttgatgg
     3361 ggagctctcg tttcaacgtt ttctcaagcg gggtagtcat aaggccggtc cgatattaac
     3421 gccgcggggt gcacgctgct gcttcagtca cgatcccctg cgcccgagct gcccgacacc
     3481 ccggagccgc ccattgagta gcgcgccccc agccagatcc cgaatggcgc accgggtccg
     3541 cggaactgct cagtgcgcgg atccactccc gcggtcggcc cgaacgtatg gttcgggccg
     3601 gtgaagaagt tctcaccgag gatgccgaag ttcgagtagc ggcgatcgag gagattcgag
     3661 actcgcgcga agatctcgag cgagtcggca ggtcgccact gcgcatccag gttcaccagc
     3721 gtatagcctg gaaccttgcc gccggcatcc agattgtttt catcgccccg ggggtacgag
     3781 ctgctgttca cgagcacatt ggtgccgacc gagacgtgac tcgagatggc gtaatccaga
     3841 cgcagcttca gcgcatgggt aggaattccc gggatgtgat cgccgggcaa cacctggatg
     3901 cccccatccg catcggcagt cgagttggat ggactgcttt cgctgaatgg tgtgcggaag
     3961 gtcgcatcgg tgtaggtgta atgggtcgcc agagacaggg attgaatctg tgtgcgcaat
     4021 gacagctcta cgccctggcg gcgggtggtg ccgacattgg aaaaatagcc tgccgtgctc
     4081 gcgccggccg ccgtgctgat gaattcgatg tcattgtcga gttgtgtgcg gtacagggcg
     4141 gcgctccact gccagacgct tctccacgtg ccgcgcgccc cggcctcgat ggtgcgcgag
     4201 acgatctgcc gcagcgccgg gtcggccagg aaatcattcg gtagcttgca cggcgcgttg
     4261 gcatcggcac acgtcagctc gatcgcggtc ggcggtcgca cgccctcgtt gtaggtgagg
     4321 taaccggtga ggtggggatc gggattgtag ttgagtccca ccgccgggtt gaaccgcgag
     4381 aacgagtgat cgccattgag cagcggcgag aggccggttc ggtcactgat ggagatgtcg
     4441 gcgtggttgt agcgtccgga tcccgtcagc gtccacttcg gcgtcaggtg aaaagtatcc
     4501 gccgcaaaca ggcccaggta gctgctggaa gaatccgcat ccgtctccag cgcgaaatcg
     4561 tcgatcccca ccgtgttgcg gttcgccgtg aactgcgcat cctgggcgtc ctgggtgaag
     4621 cgcgcatggc cggcatcgat gctggtgccg atcgtgagct gattcgaatg cgcgcggggt
     4681 cctgcggtca gaaccagctg cagacccgcc ccatagctgc gttgatcgat gacggatcga
     4741 tcattggtgg cctggacctg atcgatcgcg ccggtgttcg ggtcgatgga gccatagtca
     4801 tcgttcacat cgctcgccac gctctggttc ctgtactgcc ggtagtacgc gttgccgccg
     4861 agcagcaggc tgtcgctgag aaaccggctg cccttgagcg tcacgaaggc gagcttgttg
     4921 atattcaaat ccggaaacgt gtacgcctgc tggatgtcat tgaggaacga cagcggcagc
     4981 gtctgggtgc cttgcaggcg attatccgca ccggtgaagc tcagatcgag gtccgtcttc
     5041 tcgttctgcc agcccacctt ggcgaacagc tgctccaccc ggctggggtt gtgttgcgcc
     5101 cagccccgat cgtcgaagaa ttcgcccgtg gcgaaataat ccaatgagcc ggcgacgccg
     5161 ccccattgcg cctgcgcggt cttgcggcca aaggagccac ccgagacctg caggccgcct
     5221 cccgggtact cggtaccgct cttggtatag agggccaggg cgccgccgag cgtattcaag
     5281 ccgaaggccg ggttggagcc gggtatgagt tgcatccccg caatcgccga ctgcggaatc
     5341 agatcccagt tcacgatgtc gccgaacggc tcattgatgc gcgcaccatc ctggaacacc
     5401 gagatgccct ggggcgtgcc gaggagcggc gaggccgtga agccgcgaaa gttgatgtca
     5461 ggctggaagg ggttgccctg ggcggcattg gccgtgatgc tgccagcgtt ctgctccatg
     5521 aaatcggtca ggttggtctg gccctggcga gcgatgtcgg tgctcgtgta cacctgcaca
     5581 ttggcaggga catcctggat cggtgtgcca agcccgggca ggggggtcgt gccgatcacc
     5641 acgacggtgg gcatctcgag cgcctcggct ggattgattg gcgtgctcgt ggagttctga
     5701 ctcgtcgtct gaggcgcggg cggctgcgtc gccggcgggg gagcggcagg ctccgccaat
     5761 gagaactgcg gcgttgtctg cgatcgcgct gccgtcgctg cagccagcag gcagaatggc
     5821 agcgcagcct tccggacccg tgaccgctga ggggcaaaca cgtgcgatca ggcttccacg
     5881 gcgagcggaa tcgcatgccc cgttcgcggg agcagaacct tcaacagcgg ccccgtcatg
     5941 agggtcgtga ggacggccat ggccaccagc atggtgaaca ctttctgggg gatgaagccg
     6001 aggtcatagc caacgttgag cgcgatcaac tccaccaggc cgcgcgtgtt catgagagat
     6061 cccagcaccc atgcgcggtg gcgatcgaac ccacagagtc gcccaccgag gtaaaccgga
     6121 aggatcttgc cggcgatggc agccgccagg atgaggccca gccattgcag atcactccac
     6181 gcggtgagac ccagcacatc ggtgcgcagg ccggtgtagc tgaagaacac cggcaggaag
     6241 aatacgagcg cgaaccggcc gacctggcgc tgccaggcgg cgacgaagct gtcgaagcga
     6301 tggaacatca gccccgcgac aaagccgccg aagctggcga agactccgag ctgataggtg
     6361 cagattccca tgccgaacag caggacgatg acgatggcca tcagattcgg cggcacctcg
     6421 ccctcgcgca gccgctggcg cgagaccagc caggcggcca ggggccgcag cacgagccag
     6481 cttgcggcgc caaacagcgc gagcccaccc atctgcagca gcatgccact gccggaaaag
     6541 cgcgccgctg cgagcgcgga gattcccgcc agcatgatcc agccggcgac gtcattgacg
     6601 gccgccgcgg agatggcgat gaccccgatc tcggtacgcg tgaggccata ttcgcgcaga
     6661 atcctcccca gcaccggcaa tgccgtgatc gccagcccca ccgcaaagaa caggctgtac
     6721 accacggggt ccattcctgg ggcgaggtgt ggggcggaca gccgcccgat cagaagtccg
     6781 agcaggaaag ggaccgtgat cgatgagaaa gcaaccagga tcgcagtgcg tgtattgcgg
     6841 cgatccctca ggtgtgcaaa ctggaagtcc atgccgatct ggaacatcag caggatcagg
     6901 ccgagctgac tgatgatcgt gatgggcacg gaggccttgg cgccgaagag cgcatgcgag
     6961 gcttgcggga agaaataccc gaacagggag ggacccagca tgaggcccgc caggatctcg
     7021 ccgatcacgc ccggctgccc gagcgcgcgc agcacagtgt tcatgaggcg ggcagcgccg
     7081 atcatcacaa tgagctgcag cagcgtgacg aacaacaggt gctcggtttc gtggagcgag
     7141 acgtgcatgg gtcctggcag ccctcgatgc gggcgcggga gggctagtct ggggcgagag
     7201 gcccggtccc gcaacgtaga ccgcctgcga acagggccac cactttggcc ttccactgct
     7261 gctcggctac gggataatgc gctcccaatg cagatcaccg acggtcacag tgattcatcg
     7321 ctgcagcatg ctccgggctg accgggaccg gccatacggg ccgagggtgt cacgggctcg
     7381 tcttcgcgcc cgaatcggcc tggctgtgac agtactggcc ggggtggcta ctccgtgcat
     7441 cgtcggggca gcgagcgccg tcaacccggc ggtgagtttc atcgagcacg gtgtcatcga
     7501 gatgcgcacc gatccggaag cgagccggcg cgatgccgag gccgccctgc agagtttgca
     7561 gcgccaaccg gatgtggatc tggagatcca ggcccggctc atcctgtgcg actatcaggc
     7621 cgagcgcgac acccgcgcgg cgctggagca gatcgaggcc gccacggcgc ttctaccgca
     7681 ggcccagcgc aagggtatcc aggcgggcct gctcgattgc cggggcgaga tcttcgagac
     7741 ggccggcgac aacacccgcg cacgagggct gtacgagcag gcggttgccg tggcgaccag
     7801 cacgcacgat gaggagatgc tcgcgcagag cctgttctcc cgcggctatc tgctcggcct
     7861 gcagggcgaa tacgccgcag ggctggccga tctcaagcgc gcccagtcgc tcttcgaccg
     7921 cctcaacaag ccgttgcatt ccctcacgac gctcaacagc atcgcgatcc tctacaaccg
     7981 gatgggcgac tacgccgagg cgcgctcgat ctactcgcgg gcgctgactg ctcagcgcag
     8041 cgcgggcatg aagcgcgagc tcgtggtgac cctgcacaac ctgggccgcg cgagtgaaaa
     8101 cctccacgac tgggatgacg cgcggcaatc gttcagcgaa tcgctggcct tgagccgaca
     8161 gttgggctat tcgcggggcg aggcgtacgc gttgcgtggg cttgcggcgg tgtccaatgc
     8221 ctcgggcgac ccgaagggcg ctctcgagac gctcgatcgc gcgatgcagc tgcagcagaa
     8281 gaccgccgac gcacgcctgc tcgggcagat ccaacttgcg cgcggtgtcg cctaccacaa
     8341 gttgcagcgg ctaaccgaga gtgccacggc gctcgagagc gcattgcaga tctttctgcg
     8401 ggccgatgcg ctcggggagc tgaatgccac ctattcggag ctcgctgacg tatacgcaga
     8461 gagcggcaac tggcgtgcgg ccttcgagcg ccagacgcac gccaagctga cctccgagaa
     8521 gctgctgggc aatcaactcg atcagcgctt cgctgctctg aaggtggagt tcgacacggc
     8581 gagcaaggag aaagagaacg ccgcgctgat gcgcgaaaac caggccaacg agaaggccct
     8641 ggcgcaaggt cgagcagtcc gacacctcca ggccgccgtg atagcgctct ccgccgcgct
     8701 ggtcctcgtc cttgcgctgc ttgccgtgta ccagcgccgc agcgcactgc gcctgcgcca
     8761 tctcgccatg accgatgagc tcaccggtgt gcccaaccgg cgctcggtgc tgcgccgtct
     8821 cgagcccttg ttgaaggatt cgctcaagtc ctgctcggcg ctcatcatcg acatcgatca
     8881 cttcaagaag atcaacgatc agctcggcca tcccatcggc gacgaggtgc tgaaggtggt
     8941 ggccgcgcga cttcgcgcga gcgtcgtcga gcccgccttc ttcggacgcc tcgggggcga
     9001 ggagttcctc atcgtgctgc cggccaccgg gctggaggag gcgcgcatgg cggcagaaag
     9061 cttccgcgag gagatcctga ccgtggacac cgcccactgg tttggtgagg aggcgcgcca
     9121 catcacggcc agcgtcggct gcaccgtttc gactccggga gaggacagtc cgagcacgat
     9181 actcaagcgc gcggatgcgg cgctgtatgc ggcaaagcgc agcggccgga attgtgtccg
     9241 cacggagccg gcggtgcagc aggagttccc ctcgctgctg atcggaactc agtgacggag
     9301 ccggccgatc gctggggcga gcggctgccc aggcggatcg gcctgctcaa tgcgatcgcc
     9361 gtgttgatcg gcgtgacggt cggcagcggc atcttccggg tgcccgccac ggtcgccgcg
     9421 caactgcacg accccggccc ggtcatccta tgctggatac tcggcggtct cgtggcgctg
     9481 ttcggcgccc tcactctggc cgagctggcg ggtgcgctac cgcgctcggg cggcattttc
     9541 gcctacttgc tggagagctt cggacctgcg ccggccttcc tgttcggctg gacggagctc
     9601 gccgtcgtgc gcgcctcggc gctcggcgcg acggcgacga tctttgccga atacctcggc
     9661 tactttttcc cgttgagcgc tgctggagtg cgccgcgttg cggctctcgc gatcatcgtc
     9721 atcggcgcct tgaactacgt cggcgtgcgg cgcgccgctg ccgtgatgac gatcgccacg
     9781 gtcgcaaaat acgtcgccgt gatggcgctc ggcgtgctgg cattcagcgc gagtgccgcg
     9841 ggcggtgctc ggttcacgcc tgcgtggggt acgggcttgc acccctcgtt gctggtcacg
     9901 gcgctcatcc cggtgatgtg gacctacgat gggtgggccg atctgtcgtt catgggagga
     9961 gaggtgcgca acccgcagcg cacgctgccg ctggcactca ttctcggcac gatttgcgtg
    10021 atgctcgtgt atctgataat caacatcggc ttcatgcatc tgctgccgtt gccccagatg
    10081 gctcaatcga agctgatcgc cgcaaccgcc gcggagcaca tcccgctctt tggcggctcc
    10141 ggtgccgcag tgatcgctgc tgtggtgctg atctccactt tcagcggact caacggctcg
    10201 atgatgaccg gcccgcggat cttcttcgcc atggccgatc ggggactgtt cttcaacgcg
    10261 gtcgcgcgcg tatcgccgag gtttgacagc ccctcggtgg ccatctggct cgccacgaca
    10321 ctcggcgtcg tctatgtgct gcagaacgat ttcgcgcagc ttgcggaccg gttcatcctg
    10381 ggcatctggc ccttctacgc gctggccgtc gcggccgtgt tcgtcctgcg gcgcaagagg
    10441 ccggacctcg agcgccccta tcgcgtgctc ggctatcctg cggtcccggc cctctttctg
    10501 ctcgcctccc tggccatggt ggtgaatgcg ttcatcaccg atccggtgaa tacggggatc
    10561 actttcggaa tcatccttgc gggaattcca gcctactacc tgtggaggct gcgcaagcga
    10621 gtcccggggt gaatgcggtt cgcgccgcgt tggcttaaca ttgagggttt ggcacccggt
    10681 gccgcctgct acagatttct caaggattca tgcatggacc agttccgacg ggcttctctg
    10741 ctcgcacttg taacgctcac cagcgcagcc accgtggcct cagccacgac cgagacggag
    10801 gccggcggga gtcctacggc accgatcttc ggcttcacca cggcgcagac ctcggacgag
    10861 cgctcactcg aggcgcgttt cgacggcgat ctgaatgcgg cggacctcga tgcctggatg
    10921 aaaaatctgt ccgccgaagc caacaatgtc ggtgctccgc acgacaaggc caacgccgag
    10981 ttcgtgcgcg accagctcaa acagtggggt tgggatgctc agatcgaagt gttttatccg
    11041 ctgtatccca ccctgaagca gcacacgctg gagctcgtgg ccccaacccg ctttgttacc
    11101 acgctcaagg agcccgccgt ctccggtgat gccacatcga gccgaaccga cggaatgggt
    11161 ccgtacaacg tgtatggagc ggatggtgac gtcacgggtg atctggtcta cgccaactac
    11221 ggcatgcccg atgactacaa ggaccttgcg cggcggggca tcgacgtcaa gggcaagatc
    11281 gtgatcacgc gctatggcgc agggtggcga ggtctgaagc cgaagctggc ccaggagcac
    11341 ggcgcgattg gctgcatcat ctattccgac ccgcacgagg atgggtacgc gcgcggtgat
    11401 gtctatccga agggtggatg gcgaccgccc gaaggtgtac agcgcggatc cgttgccgat
    11461 atgcccgtct atgcgggcga tccgctcacc cccggtgtcg gggccacgaa gaacgcaaag
    11521 cgcctgccgc tttcacaggc gaagtcgtta ttgaagatcc cggtcatccc gatctcctac
    11581 ggcgatgcgc agccgctgct ggcggcgctc gccggtccgg tcgcaccgtc cagctggcgc
    11641 ggctccctgc cgattaccta ccacatgggc ccgggacctg cgaaggtcca tctggcgatc
    11701 agctccgact gggggcagaa gccgctctac gacgtcattg cccgcattcc tggctcccag
    11761 gatccggatg aatgggtcat gcgcggcaat caccgcgacg gctgggtgtt tggggcctgg
    11821 gatcccctct cgggccacgt ggcgatgatg gcggaagcca aggcgatcgg cgcgctgctc
    11881 aagacgggct ggcgccccaa gcgcacgctc gtgtacgcga gctgggatgg cgaggagccg
    11941 gggcttctgg gttccaccga gtgggccgag acgcatgccg aggagctgca gcacaaggct
    12001 gtgctctatc tgaattcaga taccaacacg cggggatttc tgcgggtcgg tggcagccat
    12061 tccctgcagc atctcgccga cgacgtcggt gccggagtga ccgatcccga gatgcacgtc
    12121 agcgtgctcg cgcggctgcg agcgcgcgag atggtcgaag gctacgagaa gggggcctcg
    12181 gagagcgaga agcaagaggc caagctcgcc gccgccggcg gcgacctgcc catcgaggcg
    12241 ctcggctcgg gctcggattt cacgcccttc ctgcagcacc tgggcatcgc tacactggat
    12301 gtgtcctatg gcggtgagga cgaccaggcc ggcgtgtatc actccgacta cgattcgtac
    12361 gatcactaca tccgcttcgg agatccgggg tttgtctatg gggtgacgga agcgaagacc
    12421 gtcggccacc tgatcctgcg gatggccgat gccgacgtgc tgccgatgca gtttggcggc
    12481 tttcatgatg cggtcgggcg gtatgtcagc gagttgcacg agcttgcgga ccacaagcgt
    12541 caggcggcgc aggagctcgg aaagctgctg gaccagaatg ctttcgcgct cgcgacggat
    12601 ccgacacgcc ccgttctggc gccccagcgt gagcctgagg ttccgtacct cgacctcgcc
    12661 ccgctcgaca acgcggttgc gcgtctgcag aaggatgcga agcaatacga tgagcgctat
    12721 gccgggttac tggcggggac gctgaagctg agtgccgcgc agagccggca gctcgacgct
    12781 ctgttgcgcg gtatggagca gacattgctg gattcgcgcg gcctgcctgg ccgcgactgg
    12841 tacaagcatc tgatctacgc accggggtta tataccggat acggggtcaa gaccctcccc
    12901 ggggtgcggg aagccatcga gcagaaccag tgggagcagg caaatcagta cagtgcgatg
    12961 acggccgcgg tgttgaatgc ctattgcgag cggctcgagc aggcaactca gttgctgggc
    13021 ccgaacaaaa tcgaggcgag gcagggttcg tgaagcgact gctgaccacg gcgctgctcg
    13081 tgggcgccgc gacgctgatg cccctgcgcc tggcggtggc ggcccgtcac gatcagactc
    13141 cggctgccga ggcagccgcg gacgcccgtc cgcttgctca cccgggcgcg gggccggctt
    13201 cggacgctcg ttttgtcgga cacgtgttcc tgatcgtgct cgagaatcag tcattcgacg
    13261 tcaccttcgg caagcgctcg ccggcaccct accttgcgaa aacgctgcgg gatcagggtg
    13321 ccctgctgcg caactactac ggcatcgggc actcgagcct ggacaattac atcgcgctcg
    13381 tcagcggcca ggcgccgaac gagcagaccc agggcgactg ccccattgtc acggagttca
    13441 agctcagtca gcccgcactc gatgcacacg gtcaggcgct cggcacgggc tgcgtgtatc
    13501 ccgcgatcgt gatgagcctg ccggatcagc tcgaggcggc aggtctgacc tggaaaggct
    13561 acatggagga catgggcaac gtgccggcgc gcgaaagctc gacctgcggc cgctcgccgc
    13621 ttaactccca ggagaagtcc tacgtgtccg tgcgtggtga caagtacgcg gcacgccatg
    13681 atccgttcat ctatttccac cgcatcgtcg atgacgccaa acgttgcaac agccacgtcg
    13741 tcaacctcga ccggctgccc aaggatctgc agagcatcga atcgacgccg aacttcacgt
    13801 tcatctcgcc taatctgtgc aacgacggtc acgatgcaca atgcgtcgac ggcaagcccg
    13861 gcggcttcca ggccatcgac agcttcctgc aggcatgggt cccgaggatc ctggactcac
    13921 ctgcctacaa gcaggacggc gtcctcatca ttactttcga cgaatccgac agcgcaggtc
    13981 cggaggcatc caccgcgtgc tgcggcgaga agccgctgcc cggcgcccgc caggcgcccg
    14041 gattcaacgg tcctggtggc ggcaggatcg gagccgtgct gctgtctgcg ttcatcaagc
    14101 ccggcacggt ctcaaccgtt ccctacaacc actattccct gctgcgctgg gtcgaggatc
    14161 agttcggact cgcgcacctg ggatacgccg gggaggcggg gttgcggact ttcggcagcg
    14221 atgtgttcac ggcgcgcgcc gacctgcctg cgaagagcag tccctcaccc tgagggcaga
    14281 ttgtcgggtg aggggagccg agacgaagag cgagtcgcgg aaaccatgga tagagtgctg
    14341 cagaagatac tcatccggga atttagaatt ggtgacgagc cggcactcca cgacgtcttt
    14401 ctatcggcgg tacacgagct ggcggctggc gattacaccc ctgagcagat cgatgcgtgg
    14461 gcgcccagat ttgtcgatgc ctcactctgg gcggatcgta tgcgcggaat ctcgcctttc
    14521 atagcggagc acgacggtgt cccgatcgcc tacgcggacg ttcaatctaa cggctatatc
    14581 gatcactttt ttgtctcagc tccgtatgcc aggatgggaa tagggacccg gttaatgcgg
    14641 cgcatccacg ctaccgcgac gtcaaacggc atgcgcgaat tgacctcgaa tgtcagccga
    14701 acggctcagc ccttcttcaa gcactttggc ttcacggtcg tcgaggagcg gcagcccatc
    14761 gtgcgcggga tcgccatagc caatgcattc atgagaaaaa cgctctgagg agactaggag
    14821 tgcccgctgg gctgcggagc catcggttgc ggcgagggct catcgagctt atcgcccatc
    14881 agtctgcgca cggcgacgaa gcagaccggg atgaggagca cgcccagcag tgcagcactc
    14941 agcatgcccc ccaccacggc ggtgccgatc gcgcgtcgcg catttgcgcc tgcgccgctc
    15001 gagaccgcca tgggaaacac gccgaggatg aaggcgaagg aggtcatcag gatcggccgg
    15061 aaacgcagcc gggccgcctc cagcacggcc tcgtgcaggc tttttcccgc tcgccgcagt
    15121 gtgacggcaa actcgacgat caggatggca ttcttcgccg acaagccgat gatggtgatg
    15181 agtcccacct tgaagaacac atcgttgggc atcccacgaa gcagcgccgc caccgtgctg
    15241 ccgatgagcc cgaccgggac ggccatcagg accgccgcgg gtacactcca gctctcgtag
    15301 agcgcggcga ggcacagata gaccaccaga atggagagcg cgaagagcat gggcgcctgg
    15361 gcgcccgaga gcagctcctg cagcgactgt cccgcccagt cataaccgat cgtgtgcggc
    15421 agcttgcgtg agacgatctg ctccatcgcc tgcatcgctt ctcccgagct gcgtccggga
    15481 gcgttggatc cggtgatctc caccgccgga tagccgttgt atcgggtgag cgagggggag
    15541 gcgaccaacc agtccgtccg cacgaccgcc gaaagcggga tcatcgcgtt gttggcgccg
    15601 atcacgtaga agtgcccgag cgcctctggg ctcatgcggt aggccgcatc ggcctgcagc
    15661 aacacgcgaa gcacgcgacc ttcgtacagg aaatcatccg cgtacaccgg cgccagcatc
    15721 aactggatcg cggtgtagac atcgctcacc gacaatccca tggattgggc ctgcacgcga
    15781 tccacggtga ggcgcagctg gggagcagga tcgagggtgt tgagcctgac gccggtgagc
    15841 gtcgtatcac gaccggcctc gcgcagcacg gtgtcctggg cgcgcgtgag ggcctcccgt
    15901 ccgaggccgg cccgatcctc gagaaagaag tcgaagcctc cgaactgtcc gagtccgcgg
    15961 atggtcggca ggttaaagac cgagatgcgc gcatcgcgca gcttgctcgg cagtgttgcg
    16021 gccgcccagg cgatgaagtc cgccgccgtc gtgctgcgct cgttccaggg cttcagatgc
    16081 acgaacgcgc gtcccacgtt ctcgcccgcg cccgtgaaac tcgagccggc gatgaggaat
    16141 gtgaacgaga cggccggatt cttcttcagc tccccatcga gttgctgcaa cacatcgagc
    16201 gtacgttgca acgaggctcc tggcgacagt tgcagatcga tcagtccgta cccctgatcc
    16261 tcctcgggaa gaaagctgcc gggcagcttc atgaacagga agccgccgac
//
LOCUS       DASRHN010000324        31391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2257685, whole genome shotgun sequence.
ACCESSION   DASRHN010000324 DASRHN010000000
VERSION     DASRHN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 31391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31391
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257685"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFB37_12820"
     CDS             complement(<1..306)
                     /locus_tag="VFB37_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO23378.1"
                     /translation="MTAFRLTVDGKAVPTASTFNVINPADESIVAACPQGTSQIVDAA
                     VAAARRAFPGWAATPDAERVAKLNAIADLIEKHHQELSQLITREQGKTQSGPGANLEA
                     "
     gene            404..1600
                     /locus_tag="VFB37_12825"
     CDS             404..1600
                     /locus_tag="VFB37_12825"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HZO23379.1"
                     /translation="MNASVRNAAPRRLTGAGLALGRGLAAGLLALGLIQPSAAVLPND
                     PMASVQALPATISPHWVWVNDIVFSHMANGKAMLVDGDNGRFLGELDVGFGSMHVVLS
                     LDGKVIFSPETYFSRGTRGDRTDVVTLYDPVHLAPIGEIVIPPKRSSNMPMMANATLT
                     DDGRFLLIYNFTPAQSISVVDTRSRTFAGEIDTPGCALTYPTGARSFFSVCADGTLLD
                     VRLDESGHATSQQRTAQMFQVQRDPVTEKAVRVRDTWYFVSYDGMLYPINISKSGLTP
                     GPTWSLLSAADTAQKWRPGGLQQLAAQAGLNRLYSIMHQGPRDTHKDPGKEVWVYDLA
                     RHARIQKIAMKNDSGSIQVTHDAHPLLFSAFIESNILDVYDASSGAYLRSVKDIGTTP
                     TVLVTP"
     gene            1597..2130
                     /locus_tag="VFB37_12830"
     CDS             1597..2130
                     /locus_tag="VFB37_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HZO23380.1"
                     /translation="MSWLDTLAETSARSMAKRTSRRSLLASLGALLVGATALPLLPVA
                     RGAQTGKPGGTKAAQPAGINDPTSCDYWRHCAIDGYLCGCCGGSQSACPPGTEMSAVT
                     WVGTCHNPLEGKDYVVSYNDCCGKTSCGRCFCNRNERDTPLYIPFQSNDYNWCSGSSK
                     ANVPYHCSTARIVSLAK"
     gene            2137..2610
                     /locus_tag="VFB37_12835"
     CDS             2137..2610
                     /locus_tag="VFB37_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23381.1"
                     /translation="MAEPLRVLTFCAVLLGSVSYPAIAYEPAVNYMLQCMGCHAPKGE
                     GEPGRVPSLRDTLVPFASIADGRKFLVQVPGAAQSTLSDAELAELLNWMIQTLSTAPP
                     SGTLAHYTAAEVARYRRTPLINVRAMRNRLFAQLSASRGGSAPIRTPPPPTPDQQ"
     gene            complement(2543..4312)
                     /locus_tag="VFB37_12840"
     CDS             complement(2543..4312)
                     /locus_tag="VFB37_12840"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZO23382.1"
                     /translation="MPDPKCSQGVRRGALLAACVIVGIAMMLTSHAAAAQAKACPDTA
                     AGLKLAPGFCATIFADKIGHARHLAVAPDGVLYVNTWSGRYYGNDRPPEGGFLVALQD
                     TKRTGHADVKERFGPTTKGGTGIAIYKDHLYYEVADRIERLALRSGTIAPQGEPEVIV
                     SGLPLGGDHPMHPFAIDADGMMYVDVASASNACQEKNRILKSPGINPCVELETRAGIW
                     RYDANKTGQKYSPSDRYATGIRNADGIAVDGSGHEVYSTQHGRDQLSQNWPSLYKPVE
                     GAILPAEELMHIQRGGDYGWPECYYDGVQRKLVLAPEYGGDGGRKVGVCAQKIAPVAA
                     FPAHWAPDDLLIYSAREFPARYHNGAFIAFHGSWNRAPFPQGGYNVVFQPLANGNASG
                     KCEIFADGFAGETKDPEGAAHRPTGLAVGPDGALFVSDDAHGRVYRIAYTGSGGDAAG
                     TPCPEATDAGPMSAGGGVPPEGTYPNAAAAANLPVPKGSTRASVVLGARIFAGQEGGA
                     TCTSCHGADGKGTPLGPDLTDQKWLWSDGGLAGIRKTINEGVPNPKEYRSPMPPMGGA
                     QLTADQVSAVAAYVWALSHHDSH"
     gene            4628..5590
                     /locus_tag="VFB37_12845"
     CDS             4628..5590
                     /locus_tag="VFB37_12845"
                     /inference="COORDINATES: protein motif:HMM:NF005292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZO23383.1"
                     /translation="MSDLFQEIVRAHAAIRPAVPVTPLQRSGPLSAALGCEVLLKCEH
                     LQPTGSFKVRGATNKIHLHTQAARRCGVIAASSGNHGQAVARMGAQAGIAVKVYVAAS
                     AARPKVEAIRALGAQLITVEGPPVEAELRARRDAAEQGRIYISPYNDLDVVAGQGTLG
                     IELIEQAPELDAVFISVGGGGLIGGVGTALKQLRPQARVVGVWPENSPCLLRALEAGR
                     IVEVEERETLSDGTAGAVEPGSVTLPVCRAVIDETVTVTEEEIGKAMRSLAQAEHWIV
                     EGAAGVALAGLVKQAAAYRGRRVAVVLCGRNILLESFLRAIGPG"
     gene            complement(5616..6401)
                     /locus_tag="VFB37_12850"
     CDS             complement(5616..6401)
                     /locus_tag="VFB37_12850"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO23384.1"
                     /translation="MSLFDLSGRVAVVTGSSRGIGRAVAERLAEQGANVVVSSRTAET
                     TQAVADGINSRPGGRAIAVPANISSHEELTRLIERTREAFGRIDILVCNAATNIHFGP
                     LARITDAQFRKILDNNILSNHWLIQLVSPEMISRRDGAIVIVSSIGALRGSPLIGAYN
                     ISKAADMQLARNLAVELGPHNVRVNCVAPGIIKTHFARALWENPKIAKAAESSTPLKR
                     LGEPDDVAGAVVFLSSPGASYITGQSIIIDGGTMVSSPSLGES"
     gene            6483..7007
                     /locus_tag="VFB37_12855"
     CDS             6483..7007
                     /locus_tag="VFB37_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO23385.1"
                     /translation="MDTRLRNFGFLLKDVSRRYVLRFEQRAREFSLTLPQCKVLARLE
                     NNEGVSQARLAELTEIEPMTMVRLLDRMEAEGLLERRPDPADRRARSLYLTSRAKPLL
                     EQIWQLSELTRAEVFAGIGRAERELLISLLERMHANVCALEDQPATAPAAAAARQTDL
                     KTGHAGRVRKIRTR"
     gene            7011..8090
                     /locus_tag="VFB37_12860"
     CDS             7011..8090
                     /locus_tag="VFB37_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZO23386.1"
                     /translation="MNDVARVRQPESAAPAEARPRDWRRRWRLPLMIGGPLLAALVAL
                     AFYFFTGRYQSTDDAYVQAASVAISSNVAGRVLELEVRDNQSVRQGEVLFKLDPSPFR
                     IAVEEADAQLSAARLQVESLKASYRQRQAELVSARETLDFQQHETQRQQRLLASGISS
                     QLQVDRAVHALENARAQLAAAQQQLSAVLASLNGNPDIAPDEHPMVQQAKAALDRAKL
                     NLSYTVIHAPSDGVVTRVEQLQVGTYIAAAAPVFALVSTRDVWVEANFKENQLTYMRA
                     GQPATIKVDSYPGKTFEGTVASVSPGTGSQFSVLPPENATGNWVKVVQRLPVRIELRR
                     LDARYPLHTGLSASVSVDTGHRRHL"
     gene            8137..9615
                     /locus_tag="VFB37_12865"
     CDS             8137..9615
                     /locus_tag="VFB37_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO23387.1"
                     /translation="MVSVMLASIMQALDNTIANVALPRMQGSLSATQDQMTWVLTSYI
                     IAAAIMTPLSGWLAGQIGRKRIFVFSVVGFTLASVLCAWSQSLAQIVIARLLQGLCGA
                     ALIPMSQAVLLDINPPEEHSRAMAIWVMGVTIGPIVGPALGGWLTENYNWRWVFYINV
                     PFGILSLLGILSFMHETPVRRSRFDFFGFTALSLGVGALQLMLDRGQIEDWFSSSSIW
                     IAAAVAGVSLYLFVVHMITTTETPFVSPALFKDRNFLTGNLFIFIVGVVLFATLALVP
                     SFLQDLLNYPVVLTGLVTAPRGLGTLAAMFIVGRLMKRVDVRLVIAAGFGITAISLWQ
                     MTDFYLQMDSSMVIWSGVLQGLGVGMVYVPLAALTFATLSPVYRNEGTALFSLIRNLG
                     SSIGISAVTALLIRNTQIMHSRLAEHITPYGDLLHLQPPAALTTTHGLVAMNADVTAQ
                     AQMIAYNNDFKLMLILTVAAIPLVALMRSAKPAAGEKPVVME"
     gene            9612..11096
                     /locus_tag="VFB37_12870"
     CDS             9612..11096
                     /locus_tag="VFB37_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO23388.1"
                     /translation="MSTFAKRRSQVLPRGDYWRRRSAALALACGFALLAGCTVGPDFH
                     RPIPPAADRYVAPAGPAQAEAASASPAAQEVALGEDLQQDWWKLFQSDALDEVVRRAL
                     AGNRTLTAAAATLAQAQELAAAQSGHRYPQLGVTVGTGAQKYGAEFLGSLAKPPPFTY
                     YAIGPTISYSFDYLGGTARSVEQRYALAEYQRQQLNAAYLTVTGHAVLETLRIALLQE
                     EIATVQAILDEDRENRKLVQTAYEAGSVARVDTVTAESQLASDGTLLPPLKQELSVAQ
                     HALAIVLGQAPAEVLPAGVELSKLALPQHLPVSVPSELAHRRPDILADEAQLHAASAA
                     VGVATANLYPHIDLTASTGLQSTVLGHLFEGSSSVYGVAGSLMAPLFNGGTLRAEKRA
                     ALDALKASAANYQQTVLTAFGQVADSLQALEHDAEQLDAQAHAESAARENVDLTRRSY
                     NEGNVGVLQVIDAERLYQNARLGLVRAQAQRYLDTVQLFLALGG"
     gene            11093..12268
                     /locus_tag="VFB37_12875"
     CDS             11093..12268
                     /locus_tag="VFB37_12875"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HZO23389.1"
                     /translation="MRSAASAANSRAAAPILVTLALAALSGCSLLRNPQFPGAPYPHS
                     RYITGVSWDFSTVGSQRRAHGSDLWPCTWAADSNLYCAWGDGGGFDGDNDNIGRVSLG
                     FARIEGVPTVEDPGSFSGKNVWGDAPRYAENQATVGGKVWTMISVDGTLYAYGHLWTA
                     ANSADPVHKGSEGPLSTLLWSSDLGKSWQLAPWSSPQLGSFLNFGQNNAGAPDSFVYI
                     YYSRPHDSADVFLKRVPASKLREDPTSSGAYEYLTGTRWRGRPRRWSTKESDARPIFS
                     DPKGADLTVIYDAPLGRYLATVAHNPGGQLETASAGQVGLFEAPEPWGPWSTVGYYDH
                     WGDLGSESRGDFLGLVLPTKWISADGRTLWAIFSALGQYDSFNFVRATLSVAGPGSH"
     gene            12268..13029
                     /locus_tag="VFB37_12880"
     CDS             12268..13029
                     /locus_tag="VFB37_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO23390.1"
                     /translation="MSAVIETCDLAKSYGRFGALHGIDLSMPQGSIFALVGASGAGKT
                     TAIRILLNILTPSHGRATVLGVDSRSIGPEVLARVGYVSENQLLPPRLRVGEFFEFLR
                     PCYRSWDQGLERELRERLRLPAERRIQALSHGMRLKMALACALAFRPQLLILDEPFSG
                     LDALTRDEMIGVFLREPREASILISSHELDEVERLATHVAFLDEGRLLFQGTLDALRE
                     RAREVLADPPQPGRPPPSLRSIFVAMVSAARRGQS"
     gene            13041..14675
                     /locus_tag="VFB37_12885"
     CDS             13041..14675
                     /locus_tag="VFB37_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23391.1"
                     /translation="MIRHILRKDWTLLWPLVLLVSAIQVGFEWATFKAGFFSANQVAR
                     ELVGLLSAAWIVGILAVAVAAIYEDPIPGAEQDWLIRPIRRSDLLLAKVLFVLIGVCL
                     PMLAVNVIHALALGFPTLTSFGMALYKELFVFACLLLPVMALASIGRNITELLVLGAS
                     LVVLYAAALGLSAVVFGAEACPTCDTAIVWIQHLLQHVGVFAGAIFILVLQYFRRRTE
                     VSRAVALAGLLTLVFLQLPWGLAFAAQSWLTGTGAPVRIALQLAVARVEAERGKGANR
                     PPRSPDLAHRATQALIEGNAGAVVGNLKHIAQPGNAAVKLHMGLSLKGLAADELLFAD
                     RTQFSIRDEQGRVLYRGTGSRASVALVPDESEAKAAGLAQQSVEIPRELYERAGTQAV
                     SLNVDDWLTLVAMTGQHRISAVDGELRAPEAGLCRSKAEANEIVIVCKQLGRAPVCYS
                     ATLYAPDGRHNPEVLQCTPDYRPYLPALGNMLTVSAVELPTHDASGLAHYAVAISELA
                     ESYVVLKTYEPVAHFTRALGPLPLTMKSDLSADPAQ"
     gene            14940..17669
                     /locus_tag="VFB37_12890"
     CDS             14940..17669
                     /locus_tag="VFB37_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23392.1"
                     /translation="MKTRSRSALAAVLALAFGVAAHADPAPFDLAGPNLDVKVTRGAQ
                     TLPASEVPNLSTGDHLLIHADLPPTQDAHYLMVAVFLRGATNPPPPAWFYRCETWTPK
                     CVQDGLNITVPQGAEQVLVFLAPETGGDFRTLVGAVRGRPGAFVRTSQDLNQASLDRA
                     RLEKYLASVRALAEKDPTHLKEVAPLLARSLAIKVDDKCLERTVALQAPCLMEGQNSL
                     VLNDGHSTSIVEALTNGPASDLAMEASFTPQLSYGYYSPYIASVLDIARIMDSFRTAQ
                     YQYIPALTLPQGDQLALVLNTPPSFQNPKSVLVVALPAVEQAQLPPLHAVDPKKSFCA
                     RKAALTLPVEGAPLVFSTSYARDLSLIVSGKDGKTVTLPAHVDPQQGGVVVDTSALAT
                     AGLGDRTQGSLHGFWGFEKYEGPRFELVNSREQEWELTGADSGPLIVGRDKTVRLRAT
                     SVSCVDTVKLKSADGQELRTQWSTVQPDEMEVTLPLQSAKPGAMTLLVSQFGSGTAGS
                     VHLRAFAEAAHLDRFAIHAGDNQGLLSGSRLDEVAGLVIGGVEFVPGKLTTVQGHDEL
                     AMITRDTQPANALKQGDAVTAKVTLNDGRAIDLNTSVDAPRPRVALIGKSVQASASAA
                     DSNIQLANLDELPQDARLTFSLRAQAPQAFARDDKIEVATSDESSSATLSLSNGGLTL
                     EDAKVAVATLDPTKAFGPSTFGPLQFRRITGGVTGDWQPLVTLVRLPLLRDLKCPSTP
                     DLACKLSGSSLFLVDSVSSDPQFAHPVQVPDGFPGYALPVPHPTNGLLYVKLRDDPSV
                     ISRAVLSAQVLPPTPEEAERAASRHAAAQPDSPASPANNTGGEAPAGPQPPQSTGAAP
                     PSVTSPQPSAALTQSHENAAPQLHEAAAPQLHEAAAPARPVDATQKGTSAPSQ"
     gene            complement(17773..19071)
                     /locus_tag="VFB37_12895"
     CDS             complement(17773..19071)
                     /locus_tag="VFB37_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO23393.1"
                     /translation="MYARILGIALALFANVCLAKDFVIHAGTLIDGLSDTPRHQVSIL
                     VRDDRIVSVQSGFETPSGAQVIDLANATVLPGFIDCHVHISSLLPSHTNSTEYWLTHS
                     DIDRAFDAAVFVREMLQQGFTSARDVGGGDETVAVRDAINEGKIPGPRLLVSLEPLGP
                     TGGHGDSRSGLDPRLNRSDWDNGIVDTPEQARIRVREHRRRGADLIKLMPSGGIASTG
                     DDPHQQLMTNEEMRVAVETAHALGMKVAAHIYPAGAIENAVRAGVDSVEHGSFATAQS
                     FALMKAHGTYLVPTLTVYEIYYAAARDHPELLTPGTAPKELANDLLPKRNLPLAIKSG
                     VRIAYGTDIGQGNHAMEFELLIANGMKPMDALNAATRNAADLLGVSARVGSVQAGRLA
                     DMVAVADDPLSKPDTLQHVQFVMKGGIVYRQNGQPTAQPQ"
     gene            complement(19114..19668)
                     /locus_tag="VFB37_12900"
     CDS             complement(19114..19668)
                     /locus_tag="VFB37_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23394.1"
                     /translation="MAAPTLPSVLIPIAVALLIAWRFYSRVRRTIGRQRFSAVRPWIT
                     VTLFPLLIVLLLLGNRAHTLPTLALPALLGGAVLGVALGVYGLRLTRFEQTPEGLFYT
                     PSAHLGIILSVILLLRIGYRMAQGYLAFGSSAPPPGNAQSPLTLLIFGAFAGYYVTYA
                     IGLLLWHRRVREGGLTSADNTTGS"
     gene            complement(19751..20386)
                     /locus_tag="VFB37_12905"
     CDS             complement(19751..20386)
                     /locus_tag="VFB37_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZO23395.1"
                     /translation="MGSLRMQGSAGWMCALLLVLAASLGPDRAIAGIDHLVPLDDSGI
                     WARTNQQILENGVIGLEVAGALWLGNDNDVGHAFWQSIDSSVISGVAAFGLKRAFSRA
                     RPDQGGNPNLWFRGGCCESFPSGEVTLQASFVTPFIADFARRDPWIWALEILPAYDAV
                     ARVKVREHWQTDVIAGWALGSAVGYWTTTRRVPISVQILPRGLSVGFSKRF"
     gene            complement(20495..20881)
                     /locus_tag="VFB37_12910"
     CDS             complement(20495..20881)
                     /locus_tag="VFB37_12910"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZO23396.1"
                     /translation="MANSIDKQDPRRFAYDGLDRIIHERARLSVLTSLVTHSKGLPFA
                     ELKQLCALTDGNLSRHLQVLQTAELVETCKGFEHNRPQTLCRITPQGRKRYLEYLLVL
                     EQVIRDAADAVGDPVPDNAERGLIRS"
     gene            complement(20874..21638)
                     /locus_tag="VFB37_12915"
     CDS             complement(20874..21638)
                     /locus_tag="VFB37_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23397.1"
                     /translation="MIDEPPAQAQPIWAVRPRHGMIHEPRCGCRRAAIDCTGNPHYFT
                     VQSWMSDVTDLYRALAQIDAIRGQVARGTEFRGYGPATLAMTGLMAFVAATVQAHVLR
                     SPRHDISTYLTIWILTAAVALTCISLETITRARRVHSGLAREMIHSAVEHFLPAILSG
                     FLLTVVLVRSAPQSEWMLPGLWQVVFSLGVFSSCRFLPRQMFAVGVWYLAAGLVYLAL
                     GPSEWSLSPWSMGIPFGVGQILVAAVLQFGYRQADG"
     gene            21705..22727
                     /locus_tag="VFB37_12920"
     CDS             21705..22727
                     /locus_tag="VFB37_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23398.1"
                     /translation="MARSALLSATLLAVSAAGADAPLAVTGLQRDVIFTEYSDYSRSE
                     ELVRRLFSPLNALRIDRAATRLGKTLREQAIELANERFAVYVPASAPPQGYSLLVFVP
                     PWSTDEVPAYWTSVLDSHGMIFVSAANSGNDANVFDRREPLALLAAKNLADRYPIDPR
                     HIFIGGFSGGSKVALRLALGYPDLFHGVLLNAGSDPIGDEHVPLPPADLFHRFQASMR
                     VVYVTGELDSLHLNEDTRSRQSLQRWCVFNVVAANEPWSEHKPAPAAAFGHALDLLLE
                     DASVDAGRLAQCRAHVESEMDASLGKAESLLAQGKSSAAHHLLERIDGRFGGLAAPRS
                     VALESQ"
     gene            22739..23038
                     /locus_tag="VFB37_12925"
     CDS             22739..23038
                     /locus_tag="VFB37_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23399.1"
                     /translation="MQSVRIRAIVAGIELSGPADLSSIRKAVARLLRAREQAEEAGYE
                     VQELRVATNPWAAQLHLQSRHAGLGALRDIDAMIVDAGMVLSIGPIVPPDAPPRS"
     gene            23044..23388
                     /locus_tag="VFB37_12930"
     CDS             23044..23388
                     /locus_tag="VFB37_12930"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HZO23400.1"
                     /translation="MTGKLAEATRATSFSIPVGSVDGAVHRAAVTTAAHVIVTLAQVQ
                     PAGDANFRFAAAACVPPGTPFFPVAYHVGADSLAFGVESAGLVRAAFAAVVLGGVWDG
                     ARRHTASGECPR"
     gene            23560..24282
                     /locus_tag="VFB37_12935"
     CDS             23560..24282
                     /locus_tag="VFB37_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZO23401.1"
                     /translation="MSLRLRRTRCAARTGREGTLLAALLMLFLAPALHAQTWKEGENY
                     FRIEPAQHTNVAPGKIEVTEAFSYACPFCAQFNPLVQQLKHSLPSNAELDYLPASFNP
                     SEDWPMFQRAACTAQALGILDKTHEAIFDAVWKTGELAVADPQTHQLRNPLPTIEDAA
                     RVYNRLTGVPVAKFVDTAKSFTVETKMRVDDGLMRAYQVDSTPTLIVNGKFRITVQSA
                     GGMKEMVEVARWLVTQEAHQGH"
     gene            complement(24279..25130)
                     /locus_tag="VFB37_12940"
     CDS             complement(24279..25130)
                     /locus_tag="VFB37_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZO23402.1"
                     /translation="MNRYALAAALAMVAFASNSILCRMALRGTAIDPASFTSVRLVTG
                     AVTMWALVRVTRGGLARAGDWGSALALLVYAVTFSFSYVGLTAGTGALLLFGAVQLIM
                     IAAGLIAGERISARLALGWALAAAGVAILILPGVSAPPALDAALMICAGIAWGVYSLR
                     GRRSSNALRDTAGNFVRAAPGALLVSAFWWAHRSSDTEGILLAVLSGAIASGLGYAIW
                     YTALPRLSAIAAGNAQLSVPVIAAFGGVLLFGEAITLRLSIASVLVLGGIALALRKGS
                     RSVPAAD"
     gene            complement(25127..26260)
                     /locus_tag="VFB37_12945"
     CDS             complement(25127..26260)
                     /locus_tag="VFB37_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZO23403.1"
                     /translation="MTREPGLNTRCVHSARAPDPATGALAQPIYLSTTFERDADGSYS
                     RGYRYSREGTPNRVALESCVASLEGGIGALAFSSGLAVNMALLEMLSAGDQLVAPLEA
                     YYGTLKQFREHAARRGILVELADFSSPASVQAAITSRTRMLWLETPANPLLSITDLAG
                     MSALGHEAGALVVCDNTFATPVCQRPFDFGVDVVVHSGTKYFGGHSDVLSGLLVVRQN
                     AELLERLQNWQKLAGSQLAPFDCWLLRRSIATLALRVRTQSGNAQRIAEFLHARKGVE
                     HVYYPGLADHPGHEIAAAQMGGGYGAVLSVCICGGSAAAMETARRLRLFTRATSLGGV
                     ESLVEHRASIEGPGSTTPQNLLRLSIGIEDAEDLTADLAQAIG"
     gene            26373..27464
                     /gene="mtnA"
                     /locus_tag="VFB37_12950"
     CDS             26373..27464
                     /gene="mtnA"
                     /locus_tag="VFB37_12950"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZO23404.1"
                     /translation="MKIAGTHYRTIWPTAAGAVRVIDQSRLPFELATVDLTTLEEAAT
                     AIRSMVVRGAPLIGATAAYGFALAMRADPSERAIMQAQRALLATRPTAANLAWALSRM
                     RAAIAGIAQEGRAEAAYREAASICEADIQQCHAIGLHGLELIRAAAREPRRPLNILTH
                     CNAGWLACVDWGTALAPIYLAHDAGIAVHVWVDETRPRNQGAALTAFELGGHGVPHTV
                     IADNLGGLLMQRGEVDLVIVGSDRTTAGGDVCNKVGTYLKALAARDNSVPFYAALPVS
                     TIDWSIEHGSEVPIEERSPDELTHVVGQASSGQMQRVRIAPAASRALNLAFDVTPSRL
                     VTGLITERGVCRATREGLRSLYPDFQAAS"
     gene            27461..28171
                     /locus_tag="VFB37_12955"
     CDS             27461..28171
                     /locus_tag="VFB37_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZO23405.1"
                     /translation="MTGSSARPREAALREQLVAASRALARRGLTPGKSGNISVRRDAS
                     HFIVTPTGMAYELLEPADMVVMDLEGRWFGRRRPSSEWRFHRDIFRTRPDTNAIVHTH
                     SLNATALACTGRGIPAFHYMVAAGGGEDIRCAPYRTFGSQELSDAALTALEGRRACLL
                     GNHGLLALGADLAAALDLAAELESLAAQYRLALSMGQVQLLDSGEMARVVEKFRTYGQ
                     QDTNDPGLVFAGEKLPAD"
     gene            complement(28190..28987)
                     /locus_tag="VFB37_12960"
     CDS             complement(28190..28987)
                     /locus_tag="VFB37_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO23406.1"
                     /translation="MSIRTLLVDDEDLARKGLRARLERAEDIDVIGECTNGREAIEAI
                     GRLSPDLVFLDVQMPEASGFDVLKALDKESAPYVIFVTAYDHYAVHAFEVRALDYLLK
                     PVDEQRLGAALSQARSALSSRRDETFGRHVAEALANWRPLAAGSAPAWGADRVAIPAG
                     DRLVVVRIAEINWVTAAGNYVSLHTDKKAWLLRETIATMDQRLARHGFARIHRSTIVN
                     LDRVLEVRSYGGGDFTVVLREGTELKLSRNYRASLWKTLGRDADHEN"
     gene            complement(28984..30123)
                     /locus_tag="VFB37_12965"
     CDS             complement(28984..30123)
                     /locus_tag="VFB37_12965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZO23407.1"
                     /translation="MRPYLSANRSGWRILRSGPPLARGTLFWLLQAAGWLLFGLMMLG
                     YELIWQSFLPAIVDDLVLISTGLALTTGFRWLYRSRRQQRASPAALLVTAAVLCVLGS
                     PVWYLAQTLLVQPLLPESMPRPQWLLSGLFLYYVFILATWNLLYFGAHGWMSLQIERR
                     RAERAEAMAQSARLRALQSQLEPHFLFNTLNGISSLVAARQHEGAATMIALLSDFLRV
                     TLKTGGTPQITLREEIGFVNQYLDIQKLRFGERLTFTVDVDPQALGSMVPTLLLQPLV
                     ENALKHGILPRAAGGRIAITVQTQGERLLLRIEDDGPGLPRSPTLSPGVGLSNTATRL
                     TELYGKQGSLFVGRSALGGVAVDIAIPLRFHSMLAEEPAPLMSQA"
     gene            complement(30451..30993)
                     /locus_tag="VFB37_12970"
     CDS             complement(30451..30993)
                     /locus_tag="VFB37_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23408.1"
                     /translation="MGGGYGIVTGPATDYIHGGASAAAGIAWQPNSAFPLALRLDGTY
                     NWFTPGRRLLGLNGVGYNRGERDVYGADLDVQFGLAATAIQRVYVLGGPGRYRVGTRL
                     QKLSAAPPVCTKRFCGTFPTLLAAESDITSWDPSWNVGVGWEMALDAHTALFLEARYQ
                     RIFTRGSTTQFVPIRVGLSF"
BASE COUNT         5391 a        10125 c        10362 g         5513 t
ORIGIN      
        1 ggcctccagg ttggctccgg ggccactctg ggtcttaccc tgctcgcggg tgatgagctg
       61 cgagagctcc tggtggtgct tctcgatcag gtcggcgatg gcattgagct tcgcaacccg
      121 ctcggcatcc ggcgtcgccg cccatccggg gaacgcgcga cgcgcggcgg ccacggccgc
      181 atcgacgatc tgcgaggtgc cctgcggaca agccgcgacg atgctctcat ccgcaggatt
      241 gatcacgttg aacgtggagg cagtcgggac ggccttgccg tccacggtga gtctgaaggc
      301 ggtcatgggg tgatctccgg gtcagcgctt cggcgaagag tttgcggtca tcggccgaag
      361 actggcaagc tgcgcccttc agcggccgcc ggttaatgat gaaatgaacg cgtccgtccg
      421 aaacgctgcc ccgcggaggc tcaccggggc cgggctcgcg ctggggaggg ggctcgcagc
      481 gggcctgctg gccctaggcc tcatccagcc gagcgcggcg gtgctgccga acgatcccat
      541 ggcgagcgtg caggccttgc ccgcgacgat ttcgccgcac tgggtgtggg tgaacgacat
      601 cgtcttctct catatggcca acgggaaggc gatgctggtg gacggggata acgggcgctt
      661 cctcggcgag ctggatgtcg gtttcggttc catgcacgtg gtgctctcgc tcgatggcaa
      721 ggtcatcttc tcgcccgaaa cctatttctc gcgcggcacg cgcggtgacc ggaccgatgt
      781 cgtcacccta tacgatccgg tgcatctggc gcccataggc gagatcgtga ttccgcccaa
      841 gcgctcctcg aacatgccca tgatggccaa cgcgactctc acggatgatg gccgcttcct
      901 gctgatctac aacttcaccc cggcgcaatc gatcagcgtc gtggataccc gcagccgcac
      961 gttcgccggg gagatcgata cgccgggctg cgcgctgacc tatcccaccg gtgcgcggtc
     1021 cttcttcagt gtctgcgccg acggcacgct gttggatgtg cgtctcgatg agagtggtca
     1081 tgcgacgagt cagcagcgca cggctcagat gtttcaggtg cagcgcgatc cggtcacgga
     1141 gaaggctgtg cgggtacgcg acacatggta cttcgtctcc tatgacggca tgctgtatcc
     1201 gatcaacatt tccaagtcgg gcctgacacc aggtccgacc tggtcgctgc tcagcgctgc
     1261 cgacaccgcg cagaaatggc ggccgggcgg cctgcagcag ctcgcggccc aggccggcct
     1321 caaccgtctc tactccatca tgcatcaggg gccgcgcgat acgcacaagg atcccggcaa
     1381 ggaggtgtgg gtgtacgatc tggcccggca cgcccgcatc cagaagattg cgatgaagaa
     1441 cgactccggc tccattcagg tcacgcacga tgcgcatccg ctgctgttct ccgccttcat
     1501 cgaaagcaac atcctggatg tgtacgacgc gagcagtgga gcgtatctgc gctcggtcaa
     1561 ggatatcggc accacgccga ccgtgctggt gacgccatga gctggctcga cacgctcgcc
     1621 gaaacctcgg cccgctcgat ggccaagcgc acttcgcggc gcagcctgct ggcgtccctg
     1681 ggtgcgctgc tcgtcggggc cacggcgctg ccgctgctgc cggttgcgcg cggggcgcag
     1741 accggcaagc ccggcggcac caaggccgct cagccagccg gcatcaacga tcccacgagc
     1801 tgcgactatt ggcgccattg cgcgatcgac ggctacctgt gcggatgctg cgggggatcg
     1861 cagagtgcct gtcctccggg gacagagatg tccgccgtga cctgggtggg gacttgtcac
     1921 aatccgctcg aaggcaagga ttacgtcgtt tcctacaacg actgctgcgg gaagacctca
     1981 tgcggccgct gcttctgcaa tcgcaacgag cgggacacgc cgctgtacat tccattccag
     2041 tcgaatgact ataactggtg ctccgggagc agcaaggcga acgttcccta ccactgctcg
     2101 accgcacgta tcgtctcgct cgccaagtag tgcaccgtgg ccgagccact tcgggttctc
     2161 accttctgtg cggtgttgct ggggagcgtc tcgtaccctg ctatcgccta cgagcctgca
     2221 gtgaactaca tgctgcaatg tatgggctgc catgccccca agggggaagg ggagccgggc
     2281 cgcgtgccct cactgcgcga tacgctcgtg cctttcgcat ctattgcaga cggtcgcaaa
     2341 ttcctggtgc aggtgcctgg cgcggcgcag tcgaccctgt ccgatgcgga gctggctgag
     2401 ttgctcaact ggatgatcca gacactcagc acagctccgc cttccggcac cctcgcgcac
     2461 tacaccgccg cggaagtcgc ccgctatcgg cgcaccccgc tgatcaacgt tcgcgccatg
     2521 cgcaataggc tgtttgcgca gctcagtgcg agtcgtggtg gctcagcgcc catacgtacg
     2581 ccgccaccgc cgacacctga tcagcagtga gctgagcacc tcccatcggg ggcatcgggc
     2641 tgcggtattc cttcggattc ggcacccctt cgttgatcgt cttgcgaatg cccgcgagac
     2701 cgccatcgct ccacagccat ttctggtcgg tcaggtcagg tccgagcggc gtgcctttac
     2761 cgtctgcgcc gtggcaactg gtgcacgtcg ctccgccttc ctgccccgca aagatacggg
     2821 cgcccagcac gaccgaggcg cgcgtggagc ccttgggtac cggcagattg gccgccgcag
     2881 ccgcattcgg gtaagtccct tcaggcggca ctccgccccc agcgctcatc gggccggcat
     2941 cggtcgcctc ggggcagggc gtccccgccg catcgccacc ggatccagtg taggcaatgc
     3001 gatagacccg accgtgtgca tcatctgaca cgaagagcgc gccgtccggg ccgaccgcca
     3061 gtccggtcgg acgatgagcc gcgccttccg gatccttcgt ctcgccggcg aatccgtcgg
     3121 cgaaaatctc gcacttgccg ctcgcattgc cgttggcgag cggttggaag acgacgttgt
     3181 agcccccctg aggaaagggt gcgcgattcc aggatccatg gaaggcgatg aaggcgccgt
     3241 tgtggtaccg agcgggaaat tcccgggcgg aatagatgag caggtcatcg ggtgcccagt
     3301 gcgctgggaa tgcagccacg ggcgcaatct tctgtgcgca aaccccgacc ttccgacccc
     3361 catccccgcc gtactcaggc gccagcacca gcttgcgctg cacgccatcg tagtagcact
     3421 ccggccagcc gtagtcgccg ccgcgctgga tgtgcatcaa ctcctcggca ggcaggatcg
     3481 ctccctcgac cggcttgtag agcgacggcc agttctgtga cagctgatcg cgtccatgtt
     3541 gggtcgagta cacctcatgc ccgctgccat ccactgcgat gccgtccgca ttgcggatgc
     3601 ctgtggcgta gcgatcggat ggcgagtact tctgaccggt cttgttggca tcgtagcgcc
     3661 agattcccgc gcgggtctcg agctcgacgc aggggttgat gccgggcgac ttcagtatgc
     3721 gattcttttc ctggcaggcg ttgctcgccg aggccacatc gacgtacatc attccgtcgg
     3781 catcgatggc gaacgggtgc atgggatgat ccccgccgag cggcagaccc gagacgatca
     3841 cttcgggctc gccctgcggc gcgatcgtgc cggagcgcag cgcaagccgc tcgatacgat
     3901 cggcgacctc gtagtagaga tggtccttgt agattgcgat gccggtaccg cccttggtgg
     3961 taggaccgaa gcgctccttg acatcggcat ggccggtccg cttcgtgtcc tgcagcgcga
     4021 ccaggaaacc cccttcgggc ggacggtcgt tgccgtaata gcgcccgctc cacgtgttga
     4081 catacagcac gccatcgggt gcgactgcga ggtgccgcgc gtggccgatc ttgtcggcga
     4141 aaatcgtggc acagaagccc ggcgcgagtt tcaacccggc cgccgtgtcg gggcaggcct
     4201 tcgcctgtgc ggctgctgcg tgcgaggtca gcatcatggc tattcccacg atcacgcacg
     4261 ccgccagcag agcgccgcgg cgcactccct gagaacactt tggatcaggc atggcacaac
     4321 ctcgtttggt tatcaggacc taagcaaaaa ccgtttctca tgccggcgtg agctgcgata
     4381 ccgttctgca aggcgcctcg aaggccgccc gttcacgcag tgaccccctc gctgccagct
     4441 ggatttgcgg cttcgaggtg ccaccacctg tttccgtgag caaagcgcga accgctctgc
     4501 ggcccccggg ggccgcccgt aggcctcgag ccctcagcgg cgcgatgtgc tcctataatc
     4561 gaatcatatc aatgcgccgc ggctgccgat tgcccgcgat gtgcgcagcg cggcggagga
     4621 gccccggatg agcgatcttt tccaggaaat cgtcagggcc catgcggcca tccgtcccgc
     4681 agttcctgtc acccccctgc aaagaagcgg cccgctctct gcggcgctcg gttgcgaagt
     4741 gctgctcaag tgtgagcacc tgcagcccac gggctcgttc aaggtccggg gcgcgaccaa
     4801 caagatccat ctgcacacgc aagcagcgcg gcgttgcggc gtgatcgcgg cctcgagcgg
     4861 caatcacggc caggcggtgg cacggatggg ggcccaggcg ggcattgccg tgaaggtcta
     4921 cgtcgccgcc tctgctgcgc gccccaaggt cgaggccatc cgggcgctcg gcgcgcagct
     4981 gatcacggtc gaggggccgc cggtcgaagc ggagctgcga gcgcgtcggg atgccgccga
     5041 gcagggcagg atctacatct ctccctataa cgacctggac gtggtcgccg gtcaagggac
     5101 gctgggcatc gagttgatcg agcaggcgcc ggagctggac gcggtattca tctcggtcgg
     5161 gggcggcgga ttgatcgggg gtgtcgggac tgcgctcaag cagctgcgcc cgcaggcccg
     5221 cgtggtgggc gtctggccgg aaaactcgcc ctgcctgctc cgagcgctgg aggccggcag
     5281 gatagtcgag gtcgaggagc gtgagacgct ctcggatggt acggccgggg ctgtcgagcc
     5341 gggctcggtc acgctgccgg tctgtcgcgc ggtcatcgac gagacggtca cggtcaccga
     5401 ggaagagatc ggcaaggcga tgcgcagcct ggcgcaggcc gagcactgga tcgtcgaggg
     5461 cgcggccggc gttgcgctcg cggggctcgt caagcaggca gcggcttaca ggggcagacg
     5521 agtggcagtc gtgctttgtg gccgcaacat tctgctcgag agcttcctcc gagcgatcgg
     5581 cccgggctga ttgcaccggc gctcgcccct ggagctcagc tctcgcccag tgagggactc
     5641 gagaccatcg tcccgccgtc gatgatgatg ctctggccgg tgatgtagct cgcacccggg
     5701 gagctcagga agaccaccgc gcccgccaca tcatccggct caccgagccg cttgagaggc
     5761 gtcgagctct cggcagcctt ggcgatcttc gggttctccc acaaggcacg cgcaaagtgg
     5821 gtcttgatga tgccgggagc cacacagttg acccgcacgt tgtgagggcc tagctccacg
     5881 gccagattgc gcgcgagctg catgtcggct gccttggaga tgttgtaggc gccgatcagc
     5941 gggctgccgc gcaaggcgcc gatcgacgag acgatcacga tcgccccgtc gcgccgcgag
     6001 atcatctccg gggacacgag ctggatgagc cagtgattcg acaggatgtt gttgtcgaga
     6061 atcttgcgga actgcgcatc ggtgatccgc gccagcggac cgaaatggat gttggtggcg
     6121 gcattgcaga cgagaatgtc gatccggccg aaagcctcgc gggtgcgttc gatcagccgg
     6181 gtcagctctt catgtgagga gatgttggcc ggtaccgcga ttgcgcgccc gcccgggcgg
     6241 gagttgatgc catcggctac cgcctgcgtg gtctcggcgg tgcgggatga cacgaccacg
     6301 ttggcaccct gctcggcgag gcgctcggcc accgctcggc cgatgccgcg tgaggatcca
     6361 gtgaccacgg cgacgcgccc gctgagatcg aacagggaca tgtgcgcagt atacccgcgt
     6421 agcttgatcg tgagccgatc acatgagatt atatcaggag ttattatagt aggacgatac
     6481 gaatggacac gcgcctgcgg aacttcggct tcctgctgaa ggatgtgagc cggcgttacg
     6541 tgctgcgctt cgagcagcgt gcgcgggaat tcagcctcac gctgccgcaa tgcaaggtgc
     6601 tggcgcgcct ggagaacaac gagggggtga gccaggcaag gctggcggag ctcaccgaga
     6661 tcgagccgat gacgatggtg cgtctgctcg atcggatgga ggcggagggc ctgctcgagc
     6721 gccggccgga ccccgccgat cggcgtgcgc gcagtctcta tctcacctct cgcgccaagc
     6781 cgctgctcga gcagatctgg cagttgagcg agctgacgcg ggcggaggtg ttcgccggca
     6841 tcggccgggc cgagcgcgag ctcctcatca gcctgctcga gcgcatgcat gccaacgtct
     6901 gcgcgctgga ggatcagccg gcaacagccc ccgcggcggc cgctgcccga caaaccgact
     6961 tgaagaccgg ccacgcgggc cgggttcgca aaatccggac acgctgaacc atgaacgatg
     7021 tagccagagt caggcagccg gagtcggctg caccggccga agcgcggccc cgggactggc
     7081 gccgccggtg gcgcctgccg ctcatgatcg gtggcccact gctggctgct ctcgtggcgc
     7141 tcgcgttcta ttttttcacg ggtcgctacc agagtacgga tgatgcctac gtacaggcgg
     7201 cgagtgttgc catcagctcc aacgtggcag gacgcgtcct cgagctggag gtgcgtgaca
     7261 atcagtccgt acgccagggc gaagttctct tcaagctgga tccctcgccg tttcgcattg
     7321 ccgtggagga agcggacgcg cagctttcgg ccgcgcgcct ccaggtcgag tcgctgaagg
     7381 cgagttatcg gcaacgtcag gcggagctcg tctcggcacg cgagacgctg gatttccagc
     7441 agcacgagac ccagcgccag cagcggctgc tggcctccgg catctcctcc cagctccagg
     7501 tggatcgggc ggtgcatgcg ctggagaacg cacgcgcgca gctcgccgct gcgcagcagc
     7561 agctcagcgc cgtgctcgcg agcctcaacg gcaatccgga tatcgccccc gatgagcatc
     7621 ccatggttca gcaggccaag gcggcactcg atcgggcgaa gctcaatctg tcctacacgg
     7681 tgatccatgc cccgagcgat ggagtggtca cgcgcgtgga gcagctgcag gtcgggacct
     7741 acattgccgc ggcggctccg gtattcgcgc tggtctcgac gcgggatgtc tgggtggagg
     7801 ccaatttcaa ggagaaccag ctgacgtaca tgcgcgccgg ccagcccgca acgatcaagg
     7861 tggacagcta cccgggcaaa accttcgaag gcaccgtggc aagcgtgagt ccgggcaccg
     7921 gctcgcaatt ctccgtgctg ccgccggaga atgccacggg caactgggtc aaggtggtgc
     7981 agagactgcc ggtgcgcatc gagctgcgcc gcctcgacgc gcgctacccg ctgcatacgg
     8041 gactgagcgc ttcggtgagc gtcgacacag gtcatcgccg ccacctctag gatgagcagc
     8101 gacactccag cggccgcacc gcatcgaggc ctcatcatgg tctccgtgat gctggcgtcc
     8161 atcatgcagg cgctcgacaa cacgattgcg aatgtcgctc tgccgcgcat gcaggggtcc
     8221 ctgtcggcga cgcaggatca gatgacgtgg gtgttgacct cttacatcat cgccgccgcc
     8281 atcatgaccc cgctgagcgg ctggctcgcc gggcagatcg ggcgcaagcg catcttcgtc
     8341 ttctcggtgg tgggctttac gctcgcatcc gtgctgtgcg cgtggtcgca gtcgctggcg
     8401 cagatcgtga tcgcacgact gctgcagggt ctgtgtggcg ccgcgttgat cccgatgtcc
     8461 caggccgtgc tgctcgacat caacccgccc gaggagcaca gccgcgccat ggccatctgg
     8521 gtaatgggcg tcaccatcgg tccgatcgtc ggccctgcgc tcggcggctg gctcaccgag
     8581 aactacaact ggcgctgggt gttttacatc aacgtgccgt tcggcattct ctcgctgctc
     8641 gggattctct ccttcatgca cgagacaccc gtgcgccgct cgcgattcga tttcttcggc
     8701 ttcacggcgc tcagtctcgg agtcggcgcg ctgcagctga tgctcgaccg cggacagatc
     8761 gaggactggt tcagttccag cagcatctgg atcgccgctg cggtggctgg cgtatcgctc
     8821 tacctgttcg tggtgcacat gatcacgacc accgagacac ccttcgtcag tcctgcgctg
     8881 ttcaaggatc gcaacttcct caccggcaat ctcttcatct tcatcgtcgg tgtggtgctg
     8941 ttcgcgacgc tcgccctggt gccctcgttc ctgcaggacc tgctcaacta cccggttgtg
     9001 ctgaccggtc tggtcaccgc gccgcgaggc ctcggcacgc tcgctgccat gttcatcgtc
     9061 gggcggctga tgaagcgggt ggacgtgcgt ctggtgatcg cagccggatt cgggatcacg
     9121 gcgatctcgc tgtggcagat gacggacttt tatctgcaga tggacagctc gatggtgatc
     9181 tggtccgggg tgctgcaggg tcttggcgta ggcatggtat acgtgcccct ggcggcgctg
     9241 accttcgcca cgctttcgcc ggtctatcgc aatgagggca cggcgctctt cagcctcatc
     9301 cgcaatctcg gcagcagtat tggcatctcg gccgtgacgg cgttgctgat ccgcaatacg
     9361 cagatcatgc attcgcgcct ggccgagcac atcaccccct acggagactt gctgcatctg
     9421 cagccgcctg cggccctgac gacgacccac ggcctcgtgg ccatgaatgc agatgtgacc
     9481 gcgcaggcgc agatgatcgc ctacaacaat gatttcaagt tgatgttgat cctgaccgtg
     9541 gctgcgattc cgctcgtggc cctgatgcgc agcgcgaaac cggcggcggg cgagaaaccc
     9601 gtggtgatgg aatgagtaca ttcgcgaaac ggcgatcgca ggtgctgccc cggggagatt
     9661 attggcgccg tcgcagcgcg gctctcgccc tagcgtgtgg tttcgcgctg ctggcgggat
     9721 gcacggtggg gcccgatttt catcggccga tcccgcccgc ggcagaccga tacgtggcgc
     9781 cggctggccc ggcacaggcg gaggcagcga gcgcttctcc ggctgcacag gaggtcgccc
     9841 tcggtgagga tctgcagcag gactggtgga agctgttcca gtccgatgcg ctcgatgagg
     9901 tcgttcgccg cgcccttgcg ggcaaccgga cgctgaccgc ggcggctgcc accctggcgc
     9961 aggcccagga gctcgcggcg gcgcagtccg ggcatcgcta ccctcagctc ggagtgactg
    10021 tcggcaccgg cgcccagaag tacggcgcgg agttcctcgg ttccctggcc aagccgccgc
    10081 ccttcaccta ctacgcgatc ggtcccacca tcagctactc attcgattat ctcgggggca
    10141 ccgctcgctc ggtcgagcaa cgctatgctc ttgccgagta ccagaggcag cagctcaatg
    10201 cggcctatct tactgtcacg ggtcatgcgg tcctggagac gctgcggatt gcgctgctgc
    10261 aggaggagat cgcgaccgtg caggccattc tcgatgagga tcgcgagaac cggaagttgg
    10321 tgcagaccgc ttacgaggcg gggtccgtcg cgcgcgtgga taccgtcacc gccgagagtc
    10381 agctcgccag cgatggcacc ttgctgccgc cgctgaagca ggagctgagt gtggcgcagc
    10441 atgctctggc gatcgtgcta ggacaggcgc cggcggaagt cttgccggcg ggggtcgagt
    10501 tgtcgaagct tgcgttgccg cagcatctgc cggtgagcgt gccctcggag ctcgcgcatc
    10561 gccgaccgga cattcttgcc gatgaggcgc aattgcatgc ggccagcgcc gctgtcgggg
    10621 tggcgacggc caacctttat ccgcacatcg atctcaccgc ttccacgggc ctgcagtcca
    10681 cggtgctggg acatctcttc gagggctcga gcagcgtgta tggcgtcgca ggctcgctga
    10741 tggcgccgct gttcaatggc ggcacgctgc gcgccgagaa gcgtgcggcg ctcgatgcgc
    10801 tcaaggcaag cgctgccaac tatcagcaga ccgtcctcac cgcctttggc caggtcgccg
    10861 attcgctgca ggctctggag cacgatgcgg aacagctcga cgcccaggca cacgccgaga
    10921 gtgcggcgcg cgagaacgtg gatctgacgc gccgcagtta caatgagggg aatgtcggag
    10981 tgctgcaggt gatcgatgcg gagcggctct accagaatgc ccggctcggt ctggtgcgcg
    11041 cgcaagcgca acgctatctg gacaccgtac aactatttct ggccctcggc ggatgaggtc
    11101 agccgcctca gccgcgaact cgcgtgcggc ggcaccgatc ctggtgaccc tggcactggc
    11161 cgcactcagc gggtgcagcc tgctgcgcaa tccgcagttc cccggtgccc cctatccgca
    11221 tagccgttac atcaccgggg tgagctggga tttctccacc gtgggcagcc agcgtcgcgc
    11281 gcatggcagc gatctgtggc cctgcacgtg ggcggccgac tccaacctgt attgcgcgtg
    11341 gggcgatggc ggcggcttcg atggggacaa tgacaacatc ggcagggtca gcctcggatt
    11401 tgcccgcatc gaaggcgtgc ccaccgtgga ggaccctgga agcttcagcg gcaagaacgt
    11461 gtggggcgat gccccgcgct atgccgagaa tcaggcaacg gtcgggggca aggtgtggac
    11521 gatgatcagt gtcgacggca cgctctacgc ctatgggcat ttgtggaccg cagccaatag
    11581 cgcagatccg gttcacaagg gcagcgaagg cccgttgagc acgctgctgt ggtccagcga
    11641 tctcggcaag agctggcagc tcgccccctg gagcagcccg cagctgggat ccttcctcaa
    11701 cttcgggcag aacaacgccg gggcgcccga ttcgttcgtg tacatttact acagccgacc
    11761 ccacgacagc gccgatgtct ttctcaagcg ggtgccggca tcgaagctgc gtgaggatcc
    11821 gacgagctcg ggtgcctacg agtatctgac gggcacccgc tggcggggca ggccgcggcg
    11881 atggtccacg aaggagagcg atgcgaggcc gatcttctcc gatccgaagg gcgccgatct
    11941 gacggtcatc tatgacgcac cgctcgggcg ctacctggcc accgttgcgc acaaccccgg
    12001 aggccaactc gagacggcct cggcggggca ggtgggcttg ttcgaggcac ccgagccctg
    12061 gggcccctgg tcaacggtcg gctactacga tcactggggc gatctcggct ccgagtcccg
    12121 tggcgacttt ctcgggctcg tgctgccgac caagtggatc agcgctgatg gccgcacgct
    12181 gtgggcgatc ttctcggccc tcgggcagta cgactctttc aacttcgtgc gggccacact
    12241 gagcgtggcg ggcccgggat cgcactgatg tccgcggtga tcgagacctg cgacctggcc
    12301 aagagctatg gccgcttcgg cgccttgcac ggtatcgatc tgagcatgcc gcagggaagc
    12361 atctttgcgt tggtaggtgc cagtggcgca ggcaagacga ccgcgatccg gattctgctc
    12421 aacattctca cacccagcca cgggcgggcc accgtgctcg gagtcgattc gcgctcgatc
    12481 ggcccggagg ttctggcgcg cgtcggttat gtttcggaga atcaacttct gccaccgcgg
    12541 ctgcgggtgg gcgagttctt cgagttcctg cggccctgct accgcagctg ggaccagggg
    12601 ctcgagcggg agttgcgcga gcggttgcgc ctgccagccg agcggcgcat ccaggcgctc
    12661 tcgcacggca tgcgcctcaa gatggcgctg gcgtgtgcgc ttgcgttccg gccgcagctt
    12721 ttgatcctcg atgagccctt cagcgggctc gatgcgctga cgcgcgatga gatgatcggg
    12781 gtcttcctgc gcgagccacg cgaggcctcg atcctgattt cctcgcacga gctcgatgag
    12841 gtggagcggc tggccacgca tgtggcgttt ctcgatgagg ggcggctgtt gttccagggc
    12901 acgctcgatg cgctgcgtga gcgcgcccgc gaggtgcttg cagacccgcc gcagccaggt
    12961 cggccacctc cttccctgcg gagcatcttc gtggcgatgg tgagcgctgc gcggcgcggc
    13021 cagagctgac ggattcgcgg atgattcgcc acattctcag gaaggactgg acgctgctgt
    13081 ggccgctggt gctgctcgtc agcgccatcc aggtcggctt cgagtgggcc accttcaagg
    13141 caggattctt cagcgcgaat caggtggccc gcgagctcgt cggcctgctc tcggccgcct
    13201 ggatcgtggg catactggcc gtggcggtcg ccgcgatcta cgaggatccg atccccggcg
    13261 ccgagcagga ctggctcatc cggcccatcc ggcgctcgga tctgctgctg gcgaaggtgc
    13321 tgttcgtcct gatcggcgtg tgcctgccga tgctcgcggt gaatgtcatc catgcgctcg
    13381 cgctgggatt tcccacactg acctcattcg gcatggcgct ctacaaggag ctgttcgttt
    13441 ttgcgtgcct gctgctgccg gtcatggcgt tggcctcgat cgggcgcaac atcaccgagc
    13501 tgctggtgct gggtgcgagc ctcgtggtcc tgtatgccgc cgccctggga ctcagcgcgg
    13561 tggtttttgg cgcagaagcg tgtccgacct gtgatacggc catcgtatgg atacaacatc
    13621 tgctacagca tgtcggggtg tttgcggggg cgatcttcat tctcgtcctg caatactttc
    13681 gacgtcggac cgaggtatcg cgggcggttg ccctggcagg gcttctgaca ctggtattcc
    13741 tgcaactgcc ctgggggctg gcctttgccg cgcaatcgtg gctgacgggc actggcgcac
    13801 ccgtgcgcat tgccctgcag ctcgctgtgg cgagggtgga agccgaacgc ggcaagggcg
    13861 ccaatcggcc gccacggagc cctgatctgg cccaccgcgc gacccaggcc ctgatcgaag
    13921 gaaacgccgg cgcggtcgtt ggaaacctga aacatatcgc tcagccgggg aatgcagcag
    13981 tcaaactgca tatggggctg agcctgaaag gcttggccgc ggatgagctg ctgttcgccg
    14041 atcgtacgca gttctcgatc cgcgatgagc aaggccgtgt gctgtaccgc gggacgggtt
    14101 cgcgcgcgtc tgtcgcactc gtgccggatg agagcgaagc caaggctgcg ggtctggcgc
    14161 agcagtcagt ggagatcccg cgcgagctgt acgagcgcgc cggcacgcag gcagtgagtc
    14221 ttaatgtgga tgattggctg acgctcgtcg cgatgaccgg ccaacatcgg atcagtgcgg
    14281 tggatgggga gctgcgcgcg ccggaggcgg gcctgtgccg ctcgaaggcg gaagccaacg
    14341 agatcgtgat cgtctgcaaa cagctcgggc gcgcgccggt ctgttacagc gcgacgctct
    14401 acgccccgga tggccgccac aaccccgaag tcctgcaatg cacgccggac taccggccct
    14461 atctgcccgc cctgggcaac atgctgacgg tttccgcggt ggagcttccg acccacgacg
    14521 cctcggggtt ggctcactat gccgtggcga tctcggagct ggccgagtca tatgtggtgc
    14581 tgaagacgta cgagccggtg gcacacttca cgcgagccct ggggccgttg ccgttgacca
    14641 tgaagtccga tctcagcgca gacccggcac aatgaccttg gcagtcgtgg cagttgtcgg
    14701 ccagatgggg gcggtcatgc ctcgccggtg ggagttttgt tgtagctcgg ccagtcctca
    14761 tccgcgacgg cggtctgcaa cgccatcagg cccagtgccg cggccgcgac tgctcgcatc
    14821 ggccacatgc tggcgctccc ggtgacaggt cgagggactc agcaagaaat gggcgggcgt
    14881 cgtcaacctg cgacgcgtat cgcggcgatc acccggggtg aagtcgctag aatgcgaaca
    14941 tgaaaacgcg ctctcgatcc gctcttgccg ccgtgcttgc gctcgcgttt ggtgtcgcag
    15001 cgcacgctga ccccgccccg ttcgatctgg cgggacctaa cctcgatgtg aaagtgacgc
    15061 gcggtgcgca gacgctgccg gcttcagagg tgccgaacct gtccaccggc gaccacctcc
    15121 tgatccacgc ggatctgccg cccacgcagg atgcgcacta cctgatggtg gcggtgtttc
    15181 ttcgcggtgc gacgaatccg ccgccgccgg cctggttcta ccgctgcgag acgtggacgc
    15241 cgaagtgtgt gcaggacggg ctgaacatca cggtgccgca gggcgctgag caggtcctgg
    15301 tcttcctcgc tcccgagact ggaggcgatt tcagaacgct ggtgggcgcc gtgcggggtc
    15361 gcccgggtgc tttcgtgcgc acgtcccagg atctgaatca ggcttcgctt gatcgcgccc
    15421 ggctggagaa gtatctggcc tcggtgcgcg cgctcgccga gaaggacccg acgcacctga
    15481 aggaagtggc cccgctgctg gcacgcagcc tggccatcaa ggtcgatgac aagtgcctcg
    15541 agcgcaccgt cgcattgcag gcgccctgtc tgatggaagg ccagaattcg ctggtgctca
    15601 acgacggcca cagcacctcg atcgtcgagg cgctcaccaa cgggccggcg agcgacctcg
    15661 ccatggaagc gagcttcacg ccgcagctca gctacggcta ttacagcccc tacatcgctt
    15721 ccgtgctgga catcgcccgc atcatggact ccttccgcac cgcacagtac cagtacattc
    15781 cggcgctgac cctgccgcag ggggaccagc tggcgctggt gctgaacaca ccgccctcgt
    15841 tccagaatcc caagtcggtg ctggtggtcg cgctgcccgc cgtggagcag gcacagcttc
    15901 ccccattaca cgcggtcgac cccaagaaat ccttttgcgc ccgtaaagct gcgctgacgc
    15961 tgcccgttga aggcgcgccg ctggtgttct cgacttccta tgcgcgtgat ctgtcgctca
    16021 tcgtctcggg aaaggacggc aagacggtca cgctgcctgc gcatgtcgat ccgcagcagg
    16081 gcggggtcgt ggtggatact tccgctctgg cgaccgcggg gcttggagac cggacccaag
    16141 gctcgctgca cggattctgg ggcttcgaga aatacgaagg cccgcgcttc gagctcgtca
    16201 acagccgcga gcaggagtgg gagctgacgg gtgccgattc cggaccgctg atcgtgggcc
    16261 gcgacaagac cgtgcggttg cgggccacca gtgtcagctg cgtggatacc gtgaaactga
    16321 agagtgcgga tggccaggag ctgcgcacgc agtggagcac ggtgcagccg gacgaaatgg
    16381 aagtcaccct gccgctgcag agcgcaaagc ctggtgcgat gaccctgctg gtcagccagt
    16441 ttggatccgg caccgcaggg agtgtgcatc tgcgcgcctt cgccgaggcg gctcatctgg
    16501 atcgctttgc gattcatgct ggagacaacc agggcctgct ctcgggcagc aggctcgatg
    16561 aggtcgccgg cctggtcatt ggtggagtcg agttcgtacc cggcaagctg accacggttc
    16621 agggtcacga tgagctcgcg atgatcaccc gggacacgca accggcaaac gcgctgaagc
    16681 agggcgatgc ggtcacggcc aaggtgactt tgaacgatgg ccgggccatc gatctgaaca
    16741 cctctgtaga tgcgccgcgc ccgcgcgtgg cgctcatcgg caagagcgtg caggcatccg
    16801 cttctgccgc cgacagcaac atccaactcg ccaatctcga cgagcttccc caggatgcgc
    16861 gactcacgtt ttcgctgcgc gcacaggcac ctcaggcgtt tgcgcgcgat gacaagatcg
    16921 aagtggccac ttctgatgag tcgtcctcgg cgactctgag cctgagtaac ggtggcctga
    16981 cgctcgagga tgcgaaagtg gcggtcgcta cgctcgatcc gaccaaggcg ttcggaccct
    17041 ccaccttcgg tccgctgcag ttccgccgga tcaccggcgg cgtgacgggc gactggcagc
    17101 cgctggtaac tctggtgcgc ttgccgctgc tgcgtgatct caagtgtccc tccacgcctg
    17161 atctggcctg caagctgtcg ggttcgagcc tgttcctcgt cgactccgtc tccagcgatc
    17221 cgcaattcgc gcacccggtg caggtgccgg atggctttcc cggttatgcg ttgcccgtgc
    17281 cgcacccgac gaacgggctg ctgtatgtga agcttcgtga tgacccttca gtgatcagcc
    17341 gtgccgttct gagcgcgcaa gtgttgccgc cgacgccgga agaagcggag cgggccgctt
    17401 cgcgtcatgc cgccgcgcag cctgattctc cggccagccc ggcaaataac accggcggtg
    17461 aggcccctgc ggggccgcag ccacctcagt ccacgggcgc agcgccgccg tcggtgacct
    17521 caccgcaacc cagtgctgca ctcacacagt ctcacgagaa tgcggcgcca cagttacacg
    17581 aggcggctgc gccacagtta cacgaggcgg ccgcgccggc gcgccccgtg gacgccacgc
    17641 agaagggcac atcagcgccc tcgcagtgaa gcggccgggg tgtgtgctac gtgcgctgat
    17701 gcgcgcctcg cgctgatcgc ctgccccgct ggtgcctggc cgcgctgact gacaccctct
    17761 gcgtgcgctg atttactgcg gctgcgcggt aggctggccg ttctggcggt acacgattcc
    17821 gcccttcatg acgaactgca cgtgctgcag cgtatcgggc ttcgatagcg gatcatccgc
    17881 cacggccacc atgtccgcta accggcccgc ctgcaccgac ccgacccggg cggagacgcc
    17941 cagcagatcg gctgcgttgc gcgtcgctgc attcaacgca tccatgggct tcatgccgtt
    18001 cgcgatcaac agctcgaact ccatggcatg gttgccctga ccgatgtcgg tgccgtaggc
    18061 gatgcgcacg cccgacttga tggcgagcgg cagattgcgc ttgggcagga gatcgttcgc
    18121 cagctccttg ggagcggtgc ccggggtcag cagctcagga tgatcacggg ccgcggcgta
    18181 atagatctcg tagacggtga gggtgggcac cagataggtg ccgtgcgcct tcatcagtgc
    18241 gaagctctgg gcggtcgcaa acgagccgtg ctcgaccgag tccacgccgg cgcgcacggc
    18301 attctcgatt gcgccggccg gatagatgtg ggcggcgacc ttcatgccca gtgcgtgcgc
    18361 tgtttcgact gcgacgcgca tctcctcgtt ggtcatcagc tgctggtgcg gatcatcgcc
    18421 ggtcgaggcg atgcctccgg aaggcatgag cttgatcagg tcggccccgc gtcgccggtg
    18481 ctcacgcaca cgaatgcgag cctgctcggg tgtgtcgacg atgccattgt cccagtcgga
    18541 gcgattcaga cgcggatcca gcccgctgcg tgaatctcca tgaccgccgg tagggcccag
    18601 cggctcgagc gagaccagca gtctcgggcc cggaatcttg ccctcgttga tggcgtcgcg
    18661 caccgccact gtctcgtccc cgccacccac gtcgcgcgca ctcgtgaatc cctgttgcaa
    18721 catctcgcgc acgaacacgg cggcatcgaa ggctcgatcg atgtccgaat gggtcagcca
    18781 gtactcggta ctgttcgtgt ggctgggcag cagcgaggag atatgcacgt gacagtcgat
    18841 gaatccgggc agaactgtgg cattcgcgag atcgatcacc tgtgcgccac tgggcgtttc
    18901 aaatccggac tggaccgaga cgatgcgatc gtcgcggacc aggatggaga cctgatggcg
    18961 cggcgtgtcg gagagcccat cgatcagcgt cccagcgtga atcacgaagt ccttcgcgag
    19021 gcagacattg gcgaacagtg cgagagcaat gccgagtatg cgtgcgtaca cgttacgaga
    19081 ccctttttgc cgtaaagatg gaagacttcg ggctcaggag cccgtggtgt tgtccgcgga
    19141 cgtgaggccg ccttcgcgaa cccgtcggtg ccaaagcagc aggccgatgg cataggtgac
    19201 gtagtagccg gcgaacgcac cgaagatcag cagagtcagc ggactctgcg cgttgcccgg
    19261 tggcggcgcc gagctgccga atgccagata accctgtgcc atccgatagc cgattcgcag
    19321 cagcaggatc accgacagga tgatgccgag atgggcgctg ggggtataga acagtccctc
    19381 aggggtctgc tcgaagcgcg tgagccgcag cccataaaca cccagagcca cccccagcac
    19441 tgcgccgccc agcagcgctg gcagcgcgag tgtgggaagg gtatgagcgc gattgccgag
    19501 gagcagcaga acgatcagca gcggaaacag ggtcaccgtg atccagggac gaaccgccga
    19561 gaatcgctga cgcccgatcg tgcgccgcac gcgcgagtag aagcgccatg cgatcaggag
    19621 ggcaaccgcg atcgggatca gaacagaggg taaagtcggg gcggccatgc agatctccat
    19681 tcgcaagcgc gccgggcgag gcggcgcgcc gcaatcatag cgcggcggtc gcgctgccta
    19741 gcgtacgcgc tcagaagcgc ttggagaacc cgaccgatag acctcgcggc aggatctgaa
    19801 cggagatcgg cacgcggcgg gttgtcgtcc agtaaccgac cgcagagccg agtgcccagc
    19861 cggcgatgac gtcggtttgc cagtgctcgc ggaccttcac gcgcgcgacg gcatcgtatg
    19921 ccggcagtat ctccagcgcc cagatccagg gatcgcgacg tgcgaaatct gcgataaaag
    19981 gcgtcacgaa gcttgcctgc agggtgacct cgccgctcgg aaagctctcg caacatccgc
    20041 cgcgaaacca cagattcggg tttccaccct gatcggggcg cgcgcggctg aaggcgcgct
    20101 tgagcccgaa tgccgcaaca ccggaaatga ctgatgagtc gatcgactgc cagaaggcgt
    20161 gtcccacgtc gttgtcattg ccgagccaca acgcgccggc cacctccagg ccgatcacgc
    20221 cgttctcgag gatctgctgg ttcgtccgcg cccagatgcc cgagtcatcg agaggaacga
    20281 gatggtctat gcccgcaatc gcgcggtcgg ggccgagact cgctgccagc acgagcagca
    20341 gcgcgcacat ccatcccgct gagccctgca tccgtaagga gcccaaggcc gtcgcaggac
    20401 cggtttcggt ggtaacttgg tgttgtcgtg gacttggcat cgcaaagcta gcggatgaaa
    20461 gtattcggga aaagagcagg catgatcagg acaatcagct gcggatgagt ccgcgttcag
    20521 cgttgtccgg gacaggatca cccaccgcgt ccgccgcatc ccgtatgacc tgctcgagca
    20581 cgagcagata ctcgagatat cgtttgcgtc cctgaggggt gatccggcac agcgtctggg
    20641 gccggttgtg ctcgaagcct ttgcaggtct cgaccaactc tgcggtctgc agcacctgca
    20701 ggtggcggct gaggttaccg tcggtgagcg cgcagagttg cttcagctcg gcgaacggca
    20761 ggcctttcga atgcgtgacg agcgatgtca gtacgctcaa gcgagctcgc tcgtggatga
    20821 tgcgatcgag cccgtcgtac gcaaaacgac gcggatcctg cttgtcgatc gaattagcca
    20881 tccgcttgcc gatagccgaa ttgcaacacg gcggcgacca ggatctgccc gaccccgaaa
    20941 gggatgccca tcgaccaggg cgacagcgac cactcgctcg ggccgagcgc caggtacacc
    21001 agcccagcgg ccagatacca cactccgacc gcgaacatct ggcggggcag gaagcggcag
    21061 gaggagaaca caccgagact gaagacgacc tgccacaggc ccggcagcat ccactcgctc
    21121 tgcggcgcgc tgcggaccag cacgacggtc aacaggaagc cgctcagaat ggccggcaga
    21181 aagtgctcga cggccgaatg gatcatctcg cgcgcgagcc ctgagtggac gcggcgcgcg
    21241 cgcgtgatcg tttccagact gatgcacgtc aacgccacgg cagctgtcaa aatccagatc
    21301 gtgagatacg tgctgatgtc gtgccgcgga ctgcgcagca cgtgcgcttg cactgtcgcg
    21361 gcgacgaagg ccatgagccc ggtcatcgcg agcgtagcgg gaccataccc gcggaactcc
    21421 gttccacgag caacctgtcc gcggatcgca tcgatctgcg ctaatgctct gtagaggtcc
    21481 gtcacatccg acatccagct ttgcaccgta aagtaatggg gattccccgt gcagtcaatc
    21541 gcagcgcggc ggcagccaca gcgcggctca tggatcatgc catgtcgcgg ccgcaccgcc
    21601 cagatgggct gcgcctgcgc gggcggttcg tctatcatcg ttcatctgag ctgctcaagc
    21661 attactggtc cgtgcgattg atggcacgcc taccggggcg cccgatggcg cgctctgcgc
    21721 tgctttccgc cacgctactc gcggtctctg cagcgggcgc tgacgcgccg ctcgctgtaa
    21781 ccggcttgca gcgggacgtg atctttaccg agtattccga ctattcccgc agcgaggagc
    21841 tggtgcggcg gctcttcagc ccgctgaatg cgctgcgcat cgaccgggcc gcgacccgac
    21901 tgggtaagac gctccgcgag caggcaatcg agctcgccaa cgagagattc gccgtgtacg
    21961 tgcctgcgag cgcgccgccg cagggctatt cgctgctggt cttcgtgccc ccctggtcaa
    22021 ctgacgaggt ccctgcgtat tggacatcgg tactcgacag tcatggaatg atcttcgtca
    22081 gcgcggccaa ctccggcaac gatgcgaacg tcttcgatcg ccgcgagccg ctcgcgctgc
    22141 tggcggcgaa aaatcttgcg gatcgctatc cgatcgatcc acgccacatc ttcatcggag
    22201 gtttctccgg tggctcgaag gtggccttgc ggctggcgct cgggtacccg gacctttttc
    22261 atggcgtgct gctcaacgcg ggcagcgacc cgatcgggga tgagcacgtg ccgctgccgc
    22321 cggctgatct gttccaccgt ttccaggcat cgatgcgtgt ggtgtatgtg accggcgagc
    22381 tcgactccct gcacctcaat gaggacaccc gcagccggca gtcgctgcaa cggtggtgcg
    22441 tattcaacgt ggtggctgcg aacgagccct ggagcgagca taagcctgcg ccggctgccg
    22501 ccttcggtca tgctctggac ctgctgctcg aggacgcctc cgtcgatgcc ggccggttgg
    22561 ctcagtgccg cgcgcacgtg gaaagcgaga tggatgcgag cctcggtaag gcggaaagcc
    22621 tgcttgcgca aggtaagtcc tccgccgcgc atcatctgct ggagcgaatc gacggccgat
    22681 ttggcggtct ggcggcgccc cgcagcgttg cgctggaaag ccagtaagga acgcgaaagt
    22741 gcaaagcgtg cgcattcgcg cgatcgtcgc gggcatcgag ctgtccgggc ccgccgatct
    22801 gtcctcgatc cgcaaggcgg tcgcacgcct gttgcgcgct cgcgagcagg cagaggaggc
    22861 cggatacgag gttcaggagc tgcgcgtggc cacgaatcca tgggccgcgc agctgcacct
    22921 gcagagccgg catgcggggc tgggagcact gcgcgacatc gatgcgatga ttgtcgatgc
    22981 tggcatggtg ctgagcatcg ggccaatcgt gccgccggat gctccgccgc gcagctgatc
    23041 gctttgaccg gaaagctggc tgaggcaacc cgggcgacca gtttcagcat cccggttgga
    23101 tcggtcgatg gcgctgtcca tcgcgcggcc gtgaccaccg cggcgcatgt cattgtcacg
    23161 ctggcgcagg tccagccggc gggcgatgcc aatttccggt tcgccgctgc cgcctgcgtt
    23221 ccgccgggca ctccattctt tccggtcgcg tatcatgtcg gagccgattc tctcgccttc
    23281 ggggtggaat cggcaggtct ggtccgggcg gcgtttgcag ctgtcgtact cggcggtgtg
    23341 tgggacgggg ctcgacgtca taccgcttcc ggggaatgtc cgcgttgaat ctctcgggcg
    23401 cttgattggc gatgtggcga tgctcgccgt gcgtttagga aagccgctcg cggcgcgctt
    23461 gctgccagcc ccggggcgcc aagccggcga gatggcccca tatggtgatg agcgcctggt
    23521 cgaatgccgg gtgttcgatg ttcagacagg agatgcttca tgtcactgag acttcggcgc
    23581 acccgatgcg ctgcccgcac cggccgcgag ggcacgctcc tcgccgccct gctgatgttg
    23641 tttctggcgc cggcgctgca cgcgcagacc tggaaggaag gtgagaacta tttccgcatc
    23701 gagccggcgc agcacaccaa tgtcgcgccc ggcaagatcg aagtcaccga ggcattctcg
    23761 tacgcgtgtc cgttctgcgc gcagttcaat ccgctggttc agcagctgaa gcattccttg
    23821 ccatccaacg ccgagctgga ctacctgccc gcctcgttca acccgtccga ggactggccg
    23881 atgtttcaga gggcggcgtg cacggcacag gcgcttggta tcctcgacaa gacccatgag
    23941 gccatcttcg atgcggtctg gaagaccggt gagctcgcgg tcgccgatcc gcagacccac
    24001 cagttgcgca atccgcttcc aacgatcgag gatgcagcgc gcgtctacaa tcgcctcacg
    24061 ggtgtaccgg tcgccaagtt cgtggatacg gcgaagtcat ttaccgtcga gacgaagatg
    24121 cgggtggatg atgggctcat gagggcctac caggtggaca gcacgccgac cctgatcgtc
    24181 aatggcaagt tccgcatcac ggtgcagtct gccgggggca tgaaggagat ggtggaagtg
    24241 gctcgctggc tcgtgacgca ggaagcacac cagggccact aatccgcggc gggcaccgat
    24301 cgcgatcctt tgcgcaaagc gagggcgata ccgcccagca cgagaaccga cgcgatcgat
    24361 agtcgcagcg tgatcgcctc tccaaacagc aggacaccgc cgaaggcggc aatgaccggc
    24421 acactcagct gggcattgcc ggcggcgatg gcgctgagcc gcggcagggc cgtgtaccag
    24481 atggcgtagc caagtcccga ggcgatcgcg cccgagagaa ccgcaaggag gatgccttcg
    24541 gtatccgagg atcgatgagc ccaccagaac gcgctcacga gcagcgctcc gggagctgcg
    24601 cgaacaaaat tgccggcggt atcgcgcagc gcattgctgg agcgacggcc gcgcagggag
    24661 tagacacccc aggcaattcc ggcacagatc atcagagcgg catcgagcgc aggcggtgcg
    24721 gatacccctg gaaggattag aatcgccacc cctgcggccg ccagcgccca gccgagcgcg
    24781 aggcgtgcgc tgatgcgctc gcctgcaatc aggcccgcgg caatcatgat gagctgcacg
    24841 gctccgaaca gcagcagcgc accggtgccg gcggtgagcc ctacatacga gaacgagaag
    24901 gtgacggcgt acaccagcag ggccaatgcc gacccccaat cccctgcgcg agcaagccca
    24961 ccccgagtta cccgcacaag cgcccacatg gtcactgccc ctgtgaccag gcgcacgctg
    25021 gtgaaggatg ccgggtcgat cgcggtgccc cgcagcgcca tgcggcacag gatcgagttg
    25081 gaggcaaagg ccaccatcgc cagggccgcc gccagcgcgt agcgattcac ccgatcgcct
    25141 gcgccagatc ggccgtgaga tcctctgcat cttcgatgcc gatcgacagg cgcagcaggt
    25201 tctgcggcgt cgtcgagccc ggtccctcga tcgaggcgcg gtgctcgacc aggctttcga
    25261 ctccgccaag actcgttgcc cgcgtgaaca gtcgcagccg ccgcgcagtc tccattgcgg
    25321 cagcgctgcc accgcagata cagaccgaca agaccgctcc gtatccgcca cccatctgag
    25381 cggccgcgat ctcgtgaccg gggtgatctg ccagcccggg gtagtaaacg tgctccacac
    25441 ccttgcgagc gtgcaggaat tcggcgatcc gctgggcgtt gccgctctgt gtacgcactc
    25501 gcagcgccag cgttgcgatg ctccggcgca gcagccagca gtcgaacggc gccagctgcg
    25561 atccggcgag cttctgccag ttctgcagcc gctcgagcag ctccgcattc tgccgcacca
    25621 cgagcagtcc gctgagcaca tcgctgtggc cgccgaaata cttggtaccg ctgtgcacga
    25681 cgacatcgac tccgaagtcg aaaggccgct gacagaccgg cgtggcgaag gtgttgtcgc
    25741 aaactacgag ggcgcctgcc tcgtgtccga gggcgctcat gccggccagg tcggtgatgc
    25801 tgagcagggg attcgcgggg gtttcgagcc agagcatgcg cgtgcgcgag gtgatggccg
    25861 cctgcacgct ggccggactg ctgaagtcgg ccagctccac gagaatcccg cgccgcgcgg
    25921 cgtgctcacg aaactgcttg agggtgccgt aataggcttc cagcggcgcc acgagctggt
    25981 cgccggcact cagcatctcg agcagcgcca tgttgactgc caggccggag gaaaatgcga
    26041 gcgcaccgat tcccccttcg agcgaagcta cgcaggactc gagcgcgaca cggttcggag
    26101 tgccttcacg gctatatcgg tacccccgtg agtacgagcc atccgcatcc cgctcgaagg
    26161 tcgtggagag gtagatgggc tgggcgagcg ctccggtggc ggggtcgggc gcacgcgcgc
    26221 tgtgaacgca tcgggtgttc agtcctggct ctcgggtcat ggtaggcagc tcgctgctga
    26281 ggacggcgcg ccattctacg gtgcgcgatc ccgccccgca gtggcggatg cccgggcaag
    26341 cgctcccgat ggacgcatgc gatgatgagg ctatgaagat cgcgggcacc cactaccgta
    26401 cgatctggcc gaccgccgcc ggggccgtga gggtgatcga tcagtcacgg ctgcccttcg
    26461 agcttgctac cgtcgatctg accactctcg aggaggccgc gacggccatc cgcagcatgg
    26521 tcgttcgcgg cgccccgctg atcggagcca cggctgcgta tgggtttgca ttggccatgc
    26581 gcgccgatcc ctccgagcgg gccatcatgc aggcgcagcg agcgctgctg gcgactcgtc
    26641 cgaccgcagc caaccttgcc tgggctctat cgcgcatgcg tgcggcgatc gccgggatcg
    26701 ctcaggaggg gagagcggag gctgcgtacc gggaggcggc atccatttgt gaggctgaca
    26761 tccagcagtg ccacgccatc ggcctgcatg gtctggagct gattcgtgcg gcggctcgcg
    26821 agcctcgccg tccgctgaac atcctgactc attgcaatgc gggatggctg gcatgcgtcg
    26881 attggggcac cgcgctcgcg ccgatctacc tggcgcacga tgccggcatc gccgtgcacg
    26941 tctgggtaga tgaaacgcga ccgcgcaatc agggcgcagc gctcacggca ttcgagctgg
    27001 gcggtcacgg cgttccccac acagtgattg ccgacaatct gggcggcctt ctgatgcagc
    27061 ggggcgaggt ggacctggtg atcgtcggca gtgaccggac gacggccggt ggagatgtgt
    27121 gcaacaaggt cggcacttat ctcaaagcac tcgccgcccg ggataacagc gtaccgttct
    27181 acgcggcatt acctgtgagc acgatcgact ggagcatcga gcacggcagc gaagtcccca
    27241 tcgaggagcg ctcgccagat gagctgactc atgtagtggg ccaggcctcg tccggtcaaa
    27301 tgcagcgggt cagaatcgcc ccggccgcaa gtcgcgcgct caatctggcc ttcgacgtga
    27361 cgccctcccg actcgtgacc gggctcatca ccgagcgagg ggtgtgtcgg gccacacgcg
    27421 agggcttgcg cagtctgtat ccggatttcc aggctgcatc gtgaccggct caagcgcacg
    27481 tcctcgtgag gccgctcttc gcgagcagct cgtggcagcc agccgggcgc tggcgcgccg
    27541 gggtctcact cccggaaagt cgggcaacat cagcgtacgc cgcgatgcct cacacttcat
    27601 cgtgaccccg acaggcatgg cgtacgagct tctcgagccg gccgacatgg tcgtgatgga
    27661 tctcgaaggc cgctggttcg gacgccgccg gccctcgagc gaatggcgct ttcaccgcga
    27721 catctttcgt acccggcccg ataccaacgc gatcgttcac acgcactcgc tcaacgcaac
    27781 tgcactagcc tgcaccggcc ggggtatccc cgcctttcac tacatggtgg ccgcaggcgg
    27841 gggagaggac attcgctgtg cgccctatcg tacctttggc tcacaggagc tgtctgatgc
    27901 cgcgctcacg gctctcgagg gccgcagagc ctgtctactc ggcaaccacg gactgcttgc
    27961 gctgggcgcc gatctggctg ccgcgctcga tctggccgct gagctggaga gcctcgccgc
    28021 acagtaccgg cttgccctgt cgatgggtca ggtccagctg ctcgacagcg gggagatggc
    28081 gcgggtggtg gagaaattcc ggacttacgg gcagcaggac acgaacgatc ccggcctcgt
    28141 gttcgccggc gaaaagctac cggcagattg agggcccgag cctccgcatt cagttctcat
    28201 ggtcggcatc cctgccgagt gtcttccaga gcgaagcgcg gtagttgcga ctgagcttca
    28261 actccgtgcc ttcgcgcagc accaccgtga aatccccgcc gccgtatgag cggacctcga
    28321 gcacgcgatc gagattgacg atggtcgagc gatggatgcg agcgaaaccg tgccgcgcca
    28381 ggcgctggtc catcgtggcg atggtctctc gcaacagcca ggccttcttg tcggtgtgca
    28441 gggagacata gtttccggct gccgtcaccc agttgatctc cgcgatgcga accaccacca
    28501 ggcggtcccc ggcgggaatg gcaacccggt ccgcgcccca agcgggcgcg ctaccggcgg
    28561 cgagcgggcg ccagttggcg agggcttcgg caacatgacg cccgaaggtc tcgtccctcc
    28621 tgctgctgag ggcactgcgt gcctgagaga gcgcagcccc aagacgctgc tcgtccacgg
    28681 gcttcagcag gtagtccagt gctcgcacct cgaaggcatg caccgcgtaa tgatcgtatg
    28741 cggtgacaaa gatgacatag ggagccgatt ccttgtcgag cgccttcagg acatcgaatc
    28801 cggaagcctc gggcatctga acgtcgagga acacgagatc cggcgaaagc cggccgatgg
    28861 cctcgatcgc ctcccggccg ttggtgcatt cgccgatgac atcgatgtct tcggcgcgct
    28921 cgaggcgcgc gcgcaagcct ttgcgagcga gatcctcatc atcgacgagc agcgtccgga
    28981 tactcatgct tggctcatca ggggcgccgg ttcctccgcc agcatcgagt ggaacctcaa
    29041 cggaattgca atgtccaccg caacaccccc gagcgcactg cggcccacga acaggcttcc
    29101 ctgttttccg taaagctccg tgagccgcgt cgcggtgttg gacagcccta cacccggact
    29161 gagagtgggc gagcgcggca ggccaggacc gtcgtcctcg atccgcagca atagcctttc
    29221 gccctgtgtc tgcaccgtga tggcgatgcg gccgccggct gcccgcggca ggatgccatg
    29281 cttgagggcg ttctcgacca ggggttgcag cagcagggtg gggaccatgg agccgagggc
    29341 ctgcgggtcc acgtcgacgg tgaaggtgag tcgctcgccg aagcgcagct tctggatgtc
    29401 cagatactgg ttgacgaaac cgatctcctc tcgcagcgtg atctgcggcg tgcctccggt
    29461 cttgagcgtg acacgcagga agtcactcaa cagcgcgatc atcgtggcgg cgccctcgtg
    29521 ctgcctggcg gcgaccaggg aggaaatacc gttcagagta ttgaacagga aatgcggctc
    29581 gagttgtgac tgcagcgccc gcagtctggc ggattgcgcc atcgcttcgg cgcgttctgc
    29641 ccggcggcgc tcgatttgca ggctcatcca gccgtgcgcg ccgaagtaaa gcagattcca
    29701 ggtcgccagg atgaaaacgt agtacaggaa caggcccgac aggagccatt gcgggcgggg
    29761 catggactcg ggcaacaagg gctgcacgag cagcgtctgg gccagatacc acaccggcga
    29821 gcccagcaca cacaggacgg cggccgtgac caggagcgca gccggtgagg cacgctgctg
    29881 ccgccggctc cgatacagcc agcggaaccc cgtggtcagg gcaagaccgg tgctgatcag
    29941 gaccagatca tcgacgatgg ccggcagaaa gctctgccag atgagctcgt acccgagcat
    30001 catcaggccg aacagcagcc aacccgccgc ctgcagcaac cagaaaagag tgccccgcgc
    30061 gagtggcgga cccgagcgca ggatccgcca acctgatcgg tttgccgaca gatagggcct
    30121 cattcggctc acgcccgtgc gctcggccac gcgtgcaatg ttggcctcgg atgaggctca
    30181 caggaaggat aacccagaca ccgtgtccgc gcatcgccgc cagggttgcg aacagaagat
    30241 ctgcagcgct cagcgcgggt tgtcgcagta gcaaggcgct tcggcgcaag cacccccccg
    30301 cacaacgcca aggcagctat cgtactaagt tggatcgggc cagcagctca ccctatcggc
    30361 cgtcccgcgg gtccacgagc cgcagccgct cgcgggctct acgatgggag tccgagcgat
    30421 cggatcatcg ggcatggact tagatcgcct tcagaagctc aaacctactc gaatgggcac
    30481 gaactgcgta gtgctgccgc gggtgaagat gcgctgataa cgagcctcta ggaacagggc
    30541 cgtgtgcgca tccagggcca tctcccagcc gacccctacg ttccaggacg ggtcccacga
    30601 agtgatgtcg ctctcggcgg caaggagggt ggggaaggtt ccgcagaaac gcttggtgca
    30661 cactgggggg gcggcggaca gcttttgcag tcgggtcccg acgcggtagc gtccgggccc
    30721 tcccagcacg taaaccctct gaatcgcagt ggcggcgagg ccgaactgaa catcgagatc
    30781 cgctccgtac acgtcgcgct cgccgcggtt gtagcctacc ccgttgagtc cgagcagtcg
    30841 ccggcccggc gtaaaccagt tgtaggtgcc atccagacgc aaagccagcg gaaaggcgga
    30901 gttgggctgc caagcgatgc ccgccgcagc gctcgcgccg ccatgaatgt aatcggtggc
    30961 cgggccggtg acgatgccgt acccaccgcc cacgtgggcg gaaaacggtc gctctgtaag
    31021 ccggcgagag gcccatgcct ggcgtgcgaa gtcatggtca ggaggtatgg tctgcgcaga
    31081 ggatggtgct gacggattca ccgcgtcctc agtgaccgct gcctgcggac cagcgttcgg
    31141 ctccgccgca gcgcgagcga acgccgtcgt gcccaccgca agcaccacga aaagacatgc
    31201 tccaagcgca atcctaattt caggtagctt gtgcacgtcg tgcctcctgc cacactttac
    31261 acccgcactc gcaacgccct gcctgggcgg ctatatcgag cccttatcgc tgtccgtcgg
    31321 acctcgaggc ggaattctca gtgttgctcc actagctcgg agcacaggtg gatgtactgc
    31381 tgcacgactg c
//
LOCUS       DASRHN010000325         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1099276, whole genome shotgun sequence.
ACCESSION   DASRHN010000325 DASRHN010000000
VERSION     DASRHN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099276"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFB37_12975"
     CDS             complement(<1..467)
                     /locus_tag="VFB37_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO23409.1"
                     /translation="MAAKRMTVVRNGRVLDIRGHRAPPADILIDGDTIAEVGAPGLPA
                     PADASVIDASDRLIHPGLINAHTHGHGNLSKGMGDRWTLELLLTAGPWISGNRTTEDK
                     YLTTYIGALEMLMKGCTACYDLTVEFPQPTPEGLAACAKAYADAGMRAVVAPMV"
     gene            complement(480..1235)
                     /locus_tag="VFB37_12980"
     CDS             complement(480..1235)
                     /locus_tag="VFB37_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO23410.1"
                     /translation="MEVVLPITAIGLLFLIWEAVCRLFSIPAYIVPSPSAIWTETSNL
                     PATVGWHTLVTGKTVLLGFLVSVLVSLPLAVLLTSSPIVAAAIYPLLVLTQAIPKVAL
                     APILVVVLGTSELPRVVVTFLVAFFPLVLSISAGLVSVPPELIELGRACRANRWTELW
                     RIRLPFAVPFIFSGLKAAIALAVVGAVVAEFVNADAGLGYLIQTSTAFFKVQLAWGAL
                     LILSIMGIVLFQIVVAIERIFFPWSPGIDKPAL"
     gene            complement(1261..2031)
                     /locus_tag="VFB37_12985"
     CDS             complement(1261..2031)
                     /locus_tag="VFB37_12985"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO23411.1"
                     /translation="MIEIENVSKHFVSRDSDRVVALTDVSISIRRNEFVCLVGPSGCG
                     KSTLLRLVGGLLPVSRGRVVIDGQTVREPRGETGIVFQSPTLLPWATVLDNVLFPLKI
                     MRRLTRGSAEHARSLLKLVGLNGFEDKYPRELSGGMQMRTGICRALVHDPDILLMDEP
                     FGSLDALTREELTLELMRIWRERPKTVLFVTHSIPESVMLADRVVVLSARPGRITEIV
                     PVDLPRPRTFGMEARDEFQAAANRIRQLVFGARRSSVH"
     gene            complement(2042..3028)
                     /locus_tag="VFB37_12990"
     CDS             complement(2042..3028)
                     /locus_tag="VFB37_12990"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO23412.1"
                     /translation="MSLTRVRVAIAALAVLAAPSALAQDKVRFQTDWIPSGEHAMYYG
                     GWEKGIFAAEGIDVTITRGYGSGDTVTKLAGGGFDFGVADMAAVMTARARQNVPVKTI
                     AIVYSQSPHSLFVLKSSGITSFKGLEGKRIGITPGNSHKFYFPKVAERAGTDPSKITW
                     VNMDGAAMAAQLIAKNLDAAPFYSIHHYYQNKAAKAAGEEILALPFVEVGFKIYAATI
                     VATDKMIAEKPELTRRFLRAIRKTFEFARDNPEETCKLHVQKVPEVALDDCLGSLRAT
                     LTFVYNDHTQQFGWGKESPERLAFTWKAIADAQELKSDWDYKQAIDTSLVEK"
     gene            complement(3217..>3444)
                     /locus_tag="VFB37_12995"
     CDS             complement(3217..>3444)
                     /locus_tag="VFB37_12995"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO23413.1"
                     /translation="QPDRLAELFAMITERSARLMNLKDYGIDVGNPADIVVIDATSRA
                     EAVATVAPVVAAFKRGRQTVDRPRARLIRPD"
BASE COUNT          627 a         1107 c         1129 g          581 t
ORIGIN      
        1 accatcggcg ccaccaccgc gcgcatgccg gcatcggcat aggccttcgc gcaggctgcg
       61 aggccctccg gcgtcggctg cgggaactcg accgtcagat cgtagcaggc cgtgcagccc
      121 ttcatcagca tctccagcgc accgatgtag gtggtcaggt acttgtcctc ggtcgtgcgg
      181 tttccgctga tccagggacc cgccgtcagc agcagctcga gcgtccagcg gtcgcccatc
      241 cccttgctga ggttgccgtg gccgtgcgtg tgggcattga tgagccccgg gtgaatgagc
      301 cggtcgctgg cgtcgatcac agacgcgtcg gccggggccg gaaggcccgg tgcgccgacc
      361 tcggcgatgg tgtcgccgtc gatcaggatg tcggcaggcg gggcgcggtg acccctgatg
      421 tcgagcacgc gtccgttgcg gaccaccgtc atgcgcttgg ctgccatggt aggcctctct
      481 cacagcgccg gcttgtcgat gccgggcgac caaggaaaga atatgcgctc gatcgcaacg
      541 acgatctgga acagcacgat acccatgatc gacaagatca gcagcgcccc ccacgcaagc
      601 tgcaccttga agaaggcggt cgaggtctgg atgaggtagc cgagacccgc atcggcgttc
      661 acgaactcgg caaccacggc gccgaccacg gcaagggcga tggcggcctt gaggccgctg
      721 aagatgaacg gaacggcaaa gggcagccgg atgcgccaca gctccgtcca gcggttggcc
      781 ctgcaggcgc ggccaagctc gatgagctcg ggcgggaccg agacgaggcc cgccgagatc
      841 gacaggacga gcgggaagaa cgccaccaga aaggtcacga ccacgcgcgg cagttcgctc
      901 gtgccgagaa ccacgaccag aatcggcgcc agcgccacct tggggattgc ttgagtcagc
      961 accagcagcg ggtagatcgc cgctgcgaca ataggcgagg acgtgagtaa cacggccaac
     1021 ggcaggctca ccaggacgga caccaggaat cccagaagca ccgtctttcc ggtcaccagc
     1081 gtgtgccagc cgacggttgc cggcaggttg ctcgtctcgg tccagatcgc ggagggcgag
     1141 gggacgatgt aggccggaat cgagaatagc cggcacaccg cttcccaaat caggaacagc
     1201 aggccgatcg cggtgatcgg cagcaccact tccatgaccc acgccggaag cggacgcggc
     1261 tcaatggacg ctgctgcgtc gagctccgaa gacgagctgg cggatgcggt tggctgcagc
     1321 ctggaattca tctcgagcct ccatgccgaa cgttcgcggc cgcggcaggt ctacgggtac
     1381 gatttccgtg atccgccccg ggcgtgcgga cagcacgacg acgcggtctg cgagcatgac
     1441 cgattcaggg atggaatgcg tgacgaacag caccgtcttg ggccgctcgc gccagatccg
     1501 catgagctcg agcgtcaatt cctcgcgcgt cagcgcatcg agggaaccga acggctcgtc
     1561 catgaggagg atgtccggat cgtgcaccag cgcccgacaa atgccggtcc gcatctgcat
     1621 tccacccgac agctcgcgcg ggtacttgtc ctcgaagccg ttgagcccga cgagcttgag
     1681 caggctgcgg gcgtgctcag cgctgccgcg cgtgaggcgg cgcatgattt tgagcgggaa
     1741 cagcacgttg tcgagcacgg tcgcccacgg caggagggtc ggcgactgga agacgatgcc
     1801 cgtctcgccg cgcggctcgc gtacggtctg gccatcgatc accacgcggc cccgactcac
     1861 gggcagaagg ccgccgacca gcctgagcag cgtcgatttt ccgcatcccg acggtcctac
     1921 caagcacacg aactcgttgc gccggatcga gatcgagacg tccgtgagcg ccaccacgcg
     1981 atcactgtcg cgcgagacga agtgcttgga gacattctcg atctcgatca tgcggccagg
     2041 ctcatttctc gaccagagag gtatcgatcg cctgcttgta gtcccaatca gatttcaatt
     2101 cctgggcatc ggcaatggcc ttccaggtga aagcgagccg ctctgggctc tccttgcccc
     2161 agccgaactg ctgcgtgtgg tcgttgtaga cgaaggtcag ggtggcgcgc aggctgccga
     2221 ggcagtcgtc gagcgccacc tcggggacct tctgcacatg gagcttgcag gtctcctccg
     2281 gattgtcgcg tgcgaactcg aaggtcttcc tgatggcgcg caggaaacgg cgagtgagct
     2341 cgggcttctc cgcgatcatc ttgtcggtcg caacgatcgt cgcagcgtag attttgaaac
     2401 cgacctcaac gaacggcagc gcgaggatct cctccccagc ggccttggcc gccttgttct
     2461 ggtagtagtg gtggatcgaa tagaacggcg cggcgtccag attcttggcg atcagctggg
     2521 cagccatggc cgccccgtcc atgttcaccc acgtgatctt gctcggatcg gtgcccgccc
     2581 gctcggcgac cttgggaaaa tagaatttgt ggctgttgcc cggcgtgatg ccgatcctct
     2641 tgccttcgag ccccttgaag ctggtgatgc cggagctctt cagcacgaag agcgagtgcg
     2701 gtgattgcga atagacgatg gcaatcgtct tgaccggcac gttctgacgc gctcgcgccg
     2761 tcattaccgc cgccatgtcg gcgaccccga agtcgaagcc gccacccgcg agcttggtga
     2821 ccgtatcacc cgagccgtag ccgcgggtga tggtcacatc gatgccttcc gcggcaaaaa
     2881 tgcccttctc ccaaccgccg tagtacatgg cgtgctcgcc ggaggggatc caatccgtct
     2941 ggaagcgcac cttgtcctgt gcgagtgcag atggggctgc aaggaccgcc agcgcggcga
     3001 ttgcgacacg aactcgcgtg agactcatcg gcacctcctg cacgcacagc ggttggggaa
     3061 gtatgcggcg gttcgagatg ctccgcaaac cgaaagcagc tcggagccgc agcaagaagc
     3121 ccgcgaaccc tcgatctgct ctcgctttgt gcggaagtgc agcgcacctt gcctaaagcc
     3181 ggtgcattgc cgcctctcgg cgctggtccg ccgttgtcag tcgggccgga tgagtctggc
     3241 cctggggcga tcgaccgtct gccggccgcg cttgaacgcg gccacgaccg gcgcaacggt
     3301 cgccaccgcc tcggcgcggc tcgtggcgtc gatgacgacg atgtcggcgg gattgccgac
     3361 gtcgataccg tagtccttca ggttcatcag ccgcgccgac cgctcggtga tcatggcgaa
     3421 cagctctgcg aggcggtccg gctg
//
LOCUS       DASRHN010000326         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2093154, whole genome shotgun sequence.
ACCESSION   DASRHN010000326 DASRHN010000000
VERSION     DASRHN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093154"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..441
                     /locus_tag="VFB37_13000"
     tRNA            366..441
                     /locus_tag="VFB37_13000"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Lys,seq:ctt)
     gene            complement(617..991)
                     /locus_tag="VFB37_13005"
     CDS             complement(617..991)
                     /locus_tag="VFB37_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23414.1"
                     /translation="MTLAQQRELREILAAAPGATLHHGDCIGADAEAHDIAVQLGLAV
                     VIHPPSEDYQRAFKSSPFVRAPRDFLKRNKDIVRETALLVAAPAGEIEELRSGTWSTV
                     RYARRIGRPVWLILPDGRIGAR"
     gene            1441..1617
                     /locus_tag="VFB37_13010"
     CDS             1441..1617
                     /locus_tag="VFB37_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23415.1"
                     /translation="MRIAVKVPLALTMLFWSISPSMAAFWNCDVPEIDGPAGVSAIAA
                     LVSVGMIAYQRYVR"
     gene            1707..3329
                     /gene="xrtE"
                     /locus_tag="VFB37_13015"
     CDS             1707..3329
                     /gene="xrtE"
                     /locus_tag="VFB37_13015"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase E/protease, VPEID-CTERM system"
                     /protein_id="HZO23416.1"
                     /translation="MSATSVGAAWNGRSAAARTAALAALCCAELLVISLVREIPQGGP
                     TWLNPYFYFRTLGLWGLSSAVALALLSWPRRHELVQAWRRAQADHDWRAPLLLNLALF
                     GTFLAAVLMVRSGATGGGLPAPYRLALHSLLIGATVVSMAGLDVPFRTALQIATRHRT
                     NLAVAALAGMLVPILSYLSQEGWRPLAGATLELTRAILQLYEPDVVVDVLERKITIGN
                     FSVTIWQNCSGYEGIGLVTAFLSIYLWAFRANLRFPHAFLLFPIGVSTIWVLNAVRIA
                     ALTSIGAHLSPLIAVHGFHSLAGWIAFLIVTLAIMALAHRSTLISRGAVARRPAADGG
                     RLAAAYLAPLFILMVASTVTAAFAPHDIALYPLKAAAVAAVLYAYRDVYLRFTWRLSG
                     VAVLAGLAVGAAWIATDPDPASGATLGAWLAQQGTPVTIAWLVSRSLGSVVLVPIAEE
                     LAFRGFLYRWLIARKFEEVSFAHVSLIALVVSSLLFGMLHERWLAASLSGAVFALVMW
                     RERHVCAAIVAHAAANAVICAWAIGMGQWSLL"
BASE COUNT          489 a         1217 c         1143 g          615 t
ORIGIN      
        1 gcgggcgtag atcccgctgg gtccctacga gaatgtaggt gggcgccgtt tgaccgaggc
       61 cacgaccaag gccagggaca gctcccgagc ggttcttata ggcccccggg tcgttgctcg
      121 cgacgcaccc cgcagccctg cgagagagat acttacgctg cgggcgtcaa aaatcaatgt
      181 cggcggccgc tggcggcgcc tttcgcgggc ccgagacgac gccgaggctc gccttgcggt
      241 gccgatctgc acgcccgacc cgcgcctgga caggggcccg cgccggtgtt acatgatcgc
      301 gccgccgggg ccccggaaat ccaagccgag ggccccgtcg ccgctgaagc ggcgccgacg
      361 gccaggggtg tatagctcag ttggtagagc agctgactct taatcagcgg gtcgaaggtt
      421 cgagtccttc tacacccacc actcagcgtt cgcggacgac gacgccggcg tggatcgacc
      481 tcatcgcgca caccactcgt gctgacgcgc acggatgacg cgaagggctc gcagtcggcg
      541 ccgcccttga cccaggctgc gggcagcgac ggcgggcggg ccctcgcgct gggacagcgg
      601 attgtgtccc gcggggtcat cgggcgccga tgcggccgtc gggcaggatc agccacaccg
      661 gccggccgat gcggcgggca tagcgcaccg tcgaccacgt gcccgagcgc agctcctcga
      721 tctcgccggc cggcgcggcg acgagcagcg ccgtctcgcg cacgatgtcc ttgttgcgct
      781 tcagaaagtc cctgggcgcg cgcacgaagg gcgaggactt gaacgcgcgc tggtaatcct
      841 ccgaaggcgg atgaatgacg accgcaaggc cgagctgcac cgcgatgtcg tgggcctctg
      901 cgtcggcgcc gatgcagtcg ccgtggtgca gcgtcgcgcc gggtgccgct gccaggatct
      961 cgcgcagctc ccgctgctgg gcgagtgtca tacctttgcg ggttcccgtg aatccgatct
     1021 gggctggagc ctcccgatca tgttagcggc gcggggatgc cgcgccgctc gaggtgcgcc
     1081 acgcgggcgc cgttggccac gtccgatgcc ggcacgcgaa agcccgggtg gatcggcctt
     1141 cggccctgcc attgccgccg gcgcgcgcct tatagcgctg ccctggcacg gatggccact
     1201 ggccggcgaa ttttcttggc gcgggtggtg cctctacatc gcccgatcct atcgagctgc
     1261 acgctcagcc tggcagaccg ccccacgtgc cgcagcgggg cgaatcgttg ccatccgcgt
     1321 caggccccaa cagcgttcaa atttaccgtt acttctgttg caagaggacg cacccgctaa
     1381 cgaggcgtta gcctcaccgt cggcctcaca aaactgagag aggcggcagg gagaacacac
     1441 atgcgcatcg cagtgaaagt accgctggca ctcaccatgc tcttctggtc gatctcgcct
     1501 tcgatggccg cattctggaa ctgcgacgtt ccggaaatcg acggccccgc cggagtgtcg
     1561 gcgatcgccg cgctcgtttc ggtcggcatg atcgcctacc agcgctacgt gcggtgacgg
     1621 ggaggggcac gggacgcgca ccgggcacgg gcgcgtccgc gaccccgagc caccatccgt
     1681 tcgatcaaag acggcctgac agacctatga gcgctacttc ggtcggcgca gcctggaacg
     1741 gccgttctgc tgcagccaga accgcagcct tggctgcctt gtgctgtgcc gagctgctcg
     1801 tcatttccct ggtccgggaa attccccagg gcggtcccac ctggctcaat ccatacttct
     1861 actttcgcac gctcggcctg tggggcctgt ccagcgccgt cgccctcgcg ctcctgtcgt
     1921 ggccgcgccg gcatgagctc gtgcaggcgt ggcgcagggc gcaggccgat catgattggc
     1981 gcgcgcccct gcttctcaac ctggccctgt tcgggacgtt cctggctgca gtcctcatgg
     2041 tcagatcggg cgcaaccgga ggcggcctgc cggcgcccta ccggctcgcc ctccacagcc
     2101 tgctgatcgg tgccacggtg gtgtcgatgg cggggctcga cgtgcccttt cgtacggccc
     2161 tgcaaatcgc cacgaggcac cgcaccaatc tcgccgtggc cgcgctggcc ggcatgctcg
     2221 tgccgatcct gtcctacctg tcccaggagg ggtggcgacc gctcgcgggc gcaaccctgg
     2281 aactgacacg ggccatcctg cagctttacg agccggacgt cgtcgtcgac gtcctcgagc
     2341 gcaagatcac gatcggcaac ttctcggtca cgatctggca gaactgctcg ggctacgaag
     2401 gcatcggact ggtgacggcc ttcctgtcga tctacctctg ggccttccgc gccaatctgc
     2461 gctttcctca cgcctttctg ctcttcccga tcggcgtgag cacgatctgg gtgctcaacg
     2521 ccgtccgaat cgcggcgctg accagcatcg gtgcccatct gtcgcctctc atcgcggtgc
     2581 acggatttca ttcgctggcc ggctggatcg cctttctcat cgtcacgctc gcgatcatgg
     2641 cgctcgccca ccgctcgacg ctgatcagtc gcggcgcagt ggcccgacgg ccggcagcgg
     2701 acggcggccg tctcgccgcc gcgtatctcg ctccgctctt catcctgatg gtggcctcga
     2761 ctgtcactgc cgccttcgcg cctcacgaca tcgctctcta cccgctcaag gcggcggccg
     2821 ttgcagccgt gctctacgcc taccgtgacg tctatctccg gttcacctgg cgcctctccg
     2881 gcgttgccgt gctcgccggg ctggcggtcg gcgcggcgtg gatcgccacc gacccggacc
     2941 cggcatcggg cgcgacgctg ggcgcatggc tcgcccagca gggcaccccc gtgacgatcg
     3001 cctggctcgt gtcccgctcg ctcggaagcg tcgtcctggt gccgatcgcc gaggagctgg
     3061 cgttccgggg gttcctctat cgctggctca tcgcgcgcaa attcgaggag gtatcgttcg
     3121 cgcacgtctc gctgatcgcg ctcgtcgtca gctcgctgct gttcggcatg ttgcatgagc
     3181 gctggctcgc agcgagcctg tccggagccg tgtttgcgct cgtcatgtgg cgcgagcgcc
     3241 acgtctgtgc agcaatcgtc gcgcacgcgg ccgcgaatgc cgtcatttgc gcgtgggcca
     3301 ttggcatggg ccaatggtcg ctgctctagg gggcgccccg gcagcggcgc caacgacaag
     3361 gggcgccgcg agggcgcccc tgattggtgt cgacggaagg acccgctgct atttcaacgg
     3421 tacgaggcgc tcctcgcggt gcagcttgag gctgagaccc agcc
//
LOCUS       DASRHN010000327         5598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1100587, whole genome shotgun sequence.
ACCESSION   DASRHN010000327 DASRHN010000000
VERSION     DASRHN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100587"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            695..1342
                     /gene="ccmA"
                     /locus_tag="VFB37_13020"
     CDS             695..1342
                     /gene="ccmA"
                     /locus_tag="VFB37_13020"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HZO23417.1"
                     /translation="MRERRLQAENLHLWRGETHVLRGLRLALNSGECLQITGGNGAGK
                     TSLLRTLAGLMYPEEGCVLWDGEDVRKDMQGFHAHLAYIGHEPPLKLDLTARENLHYW
                     VGVRRPVSRAELQDALMRVGAGDWLDRPVRTLSAGQKRRVALAGLALMNAPLWLLDEP
                     TTNLDATGQRLVGAMIEAQLAQGGLVVAAMHHELLVASAAVRRLELVGTLERNRA"
     gene            1335..2057
                     /gene="ccmB"
                     /locus_tag="VFB37_13025"
     CDS             1335..2057
                     /gene="ccmB"
                     /locus_tag="VFB37_13025"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZO23418.1"
                     /translation="MLESLSTPAMALRRPNTSAWSAAQLMVARDIRLAFRKRGQLVQP
                     LAFFAIVTTLFPLGISPELSRLRDVAPGVLWVSALLASVLALELLFRDDAQDGTLEQQ
                     ALSGQSLTWLLFAKTVAHWLLTGLPLSLMAPLAALSLGAPIVSLGGIVISVALGSVAL
                     SLIGSIAAALTLGVKRSGALLSLLTLPLSMPVLIFGARATELAMTGAAFGPPLYLLGA
                     LAVLGVTLARLAAAAAVRINLE"
     gene            2068..2805
                     /gene="ccmC"
                     /locus_tag="VFB37_13030"
     CDS             2068..2805
                     /gene="ccmC"
                     /locus_tag="VFB37_13030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HZO23419.1"
                     /translation="MTWTWFHRLGSPPHAYALAARLTPWFAWTAAALIVAGLYGGLVL
                     APPDYQQGDDFRIIYVHAPSAWMSLMVYTTMAVSAAVGLIWRMKVAHAVAASCAAIGA
                     SFTFAALVTGSLWGERMWGTYWVWDPRLTFELILLFQYMGYMGLRSAFDDVQRADRAS
                     AVLAVVGVVNVPIIHYSVIWWNSLHQAPSVMQLGRPTMPAAMLIPLLMMFLGFTLLFL
                     ALLLVRMRAEILDRERTATWIREAVTS"
     gene            2802..2966
                     /gene="ccmD"
                     /locus_tag="VFB37_13035"
     CDS             2802..2966
                     /gene="ccmD"
                     /locus_tag="VFB37_13035"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HZO23420.1"
                     /translation="MNVFLEMGGYAAYVWPSYGLTLGIIILNVVWARRLLARSRAEAR
                     RRLATRGEPR"
     gene            2963..3406
                     /gene="ccmE"
                     /locus_tag="VFB37_13040"
     CDS             2963..3406
                     /gene="ccmE"
                     /locus_tag="VFB37_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZO23421.1"
                     /translation="MTPRQRRMTLVLGIVVGVSIAGALALSAFRQNVTFFFDPSQVAA
                     GQVPAGEQFRLGGMVTKGSVQRTPGSLQVRFVVTDFKHNIPVVYDRVLPDLFREGQGV
                     VAHGRMQGGTFVADEVLAKHDEKYMPPAVARSLKRPPQSPNPGGA"
     gene            3403..5367
                     /locus_tag="VFB37_13045"
     CDS             3403..5367
                     /locus_tag="VFB37_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZO23422.1"
                     /translation="MMLPELGHFVLILALLLAALQAFFGIAGPALQRDRWIAAVTPAV
                     AGQFVMIATAVICLISSFVGNDFSVTYVAENSNSALPLFYRVTAMWGAHEGSLLLWIF
                     LLSCWTVAVAIGAARLPPRFSARVLGVLGLVSFGFLLFTLATSNPFLRLDPPAPDGRD
                     LNPILQDPALAIHPPILYTGYVGFAVAFAFACAAMLEGRLDQLWARWTRPWTTAAWAF
                     LTVGISLGSWWAYYELGWGGFWFWDPVENASFMPWLVGTALIHSLAVTDKRGLFKSWT
                     LLLAILAFSLSLLGTFLVRSGVLVSVHSFAADPRRGVFILAFLIIMIGGALGLYAWRA
                     PKLKSQAGFELSARESFLLFNNILLVIAAATVFAGTLAPLIADSLGLGTLSVGPPYFD
                     PTFMLSTLPLLALVSLGIHASWKKGRLGDSARTLLMMLVIALIVGVILAFGIYARGKV
                     LSLVGATLGAWIILSSLVDPVQRLRRGLSLSRSVLGMTVAHVGLGVFVISLTTVETFT
                     VERDVAMAPGQSAVVANYQFRFEGIRAIEGPNYNGVEATVLVSRHGKPMTRLYPQKRQ
                     YWVQRSATTEAAIEMHRGSNLFIALGDELGAGKWSVRFQVRPLVNFIWLGAFIMALGG
                     ACAATDRRYRTAAATATQTTVAGSREEAAG"
     gene            5364..>5598
                     /locus_tag="VFB37_13050"
     CDS             5364..>5598
                     /locus_tag="VFB37_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZO23423.1"
                     /translation="MNRFLLPLGAFALLVIVLAVGIKRSPDKGIIPSPLIGKAAPSFR
                     LPVLNDTGRTLSTADLKGRWYVFNVWGTWCVSCR"
BASE COUNT          818 a         1797 c         1814 g         1169 t
ORIGIN      
        1 ccacgtacca cacggatcca aggagcatcg atcagcgcgc tgtgatcgcc agagatgtga
       61 tccgtgacgt ctcacactga cgacttccag cacgttgact gttgcaccgg aatctgcgcg
      121 cgctgtggcg cggatcggaa ctggattcgc gccggcctca tcaactccag tcacatcggt
      181 gactgcacat gctgcccagg gccggactcg aaccggcacg gggttgcccc caagggattt
      241 tcgtacccac tacagctttc actgctgcgc gcggggcgcg catttggggt ctggacttta
      301 cctttgccct gtcgcaggcc gcaggacctg agagttaggc aggggccgtc aagtctctac
      361 actttcccgt cgcaagcagc gcggcgggct tagctcggtg ttgcagccac cctcgcgtgc
      421 tgctgtttca ccgactttga ccccattcac accggccgtt tccgtaccgg gtgctcaaag
      481 tgctcaagtc ccttgcgtct accaatttcg ccacccgggc gcgggctgca ggcgattcta
      541 cagaagccag gcgagagcgc ccgagccttg cgtgggccgg gtgccctcag gcctggccca
      601 ttgatacggg ttttaccggg tgagattctc gttctagcgc taagccactg tatttgcgag
      661 actcgcgcgc catctgctca tccccgagga ggccatgcgc gagcgccgcc tgcaagccga
      721 gaatctgcat ctctggagag gggaaaccca cgtgctccgg ggacttcgcc tcgccctgaa
      781 cagcggcgag tgcctgcaga tcaccggagg gaatggggcc ggcaagacga gtctgctgcg
      841 gacgttggcg ggattgatgt accccgaaga ggggtgcgtg ttgtgggatg gtgaggacgt
      901 tcgcaaggat atgcagggat ttcacgctca tcttgcctat atcgggcacg aacctccctt
      961 aaaattggat ctgaccgcac gcgagaacct gcactactgg gttggggtta gaaggccggt
     1021 gtcgcgcgct gagctgcagg atgccctcat gcgggtcggc gccggggact ggctcgaccg
     1081 cccggtgcgc acactctccg ccgggcagaa gcgccgcgtg gcgctcgccg ggctggcgct
     1141 gatgaatgcg cccctgtggc tcctggatga gcccacgacc aatctcgatg cgacgggcca
     1201 gcgcctggtg ggagccatga tcgaggcgca acttgcccag ggtggcctgg tcgtagcagc
     1261 catgcaccat gagcttttgg tcgcctccgc agcggtgcgg cgactcgagc tggtcgggac
     1321 gctggagcgc aatcgtgctt gagtcgctca gcacacccgc gatggcgctt cgaaggccga
     1381 acacctccgc atggagcgcg gcgcagctca tggtggcgcg ggacatccgc ctcgcgtttc
     1441 gcaagcgtgg tcagctcgtg cagccgctcg ccttttttgc gatcgtgacc acgctgtttc
     1501 cgcttgggat ctcgccggag ttgtcgcggt tgcgcgacgt cgccccgggt gtgctgtggg
     1561 tctcggctct attggcttcg gtactggcgc tcgagctcct gttccgcgac gatgcgcaag
     1621 acgggacgct cgagcagcaa gctctctcgg gccaatctct cacgtggctc ctgtttgcaa
     1681 aaaccgtggc ccattggctg ctgaccggcc tgccgctgtc gctcatggca ccgcttgcgg
     1741 ccctctcgct gggcgcgccg atcgtctcgc ttggcgggat cgtgatctcg gtcgccctgg
     1801 gaagcgtcgc gctgagcctg atcggctcca tcgccgctgc cctgacgctc ggtgtgaagc
     1861 gcagcggcgc gctgctgtct cttctgacac tgccgctgtc gatgccggtg ctgattttcg
     1921 gtgcgcgcgc tacggaactg gcgatgacgg gcgcggcctt tggcccaccc ttatatcttc
     1981 tgggtgcgct cgcggtgctc ggtgtgacgc tggcgcggtt ggccgctgcc gccgccgtgc
     2041 gcatcaatct ggagtgaagt accggacatg acctggacct ggtttcatcg gctcggctct
     2101 ccaccgcacg cgtacgctct ggcagcgcgg ctgactccgt ggttcgcctg gacggccgct
     2161 gcgctgattg ttgcggggct gtatggcggg ctggtgctgg cgccccccga ctatcagcag
     2221 ggcgatgact tcaggatcat ctacgttcat gcgcccagcg cctggatgtc gctgatggtc
     2281 tacacgacca tggcggtgag cgccgcagtg ggtttgatct ggcgcatgaa agtcgcccac
     2341 gccgtggctg cgagttgtgc ggccatcggc gcctccttca ctttcgcggc gctggtcacc
     2401 ggctcgctct ggggcgagcg catgtgggga acgtactggg tgtgggaccc gcgattgacc
     2461 ttcgagctca tccttctgtt ccagtatatg ggctacatgg gcctgcgctc tgcatttgac
     2521 gatgtgcagc gggcggatcg cgccagcgcg gtgctggcgg tcgtgggcgt ggtgaacgtc
     2581 ccgatcatcc attattcggt gatatggtgg aactcgctgc accaggcccc gtcagtgatg
     2641 cagttgggtc ggcccacgat gcccgcagcg atgttgatcc cgctgctgat gatgttcctg
     2701 ggcttcactt tgcttttcct ggccctgctg ctggtgcgga tgcgggcgga gatactggat
     2761 cgggagcgca cggcgacctg gatccgggag gcggtcacct catgaacgtg ttcctcgaga
     2821 tgggaggcta cgcggcctat gtgtggccct cgtacgggct cacgctcggg atcatcatcc
     2881 tgaatgtcgt gtgggcgagg cgattgcttg ccaggtcgcg ggccgaggca cgccgccgcc
     2941 tggctactcg aggagagccg agatgactcc gcggcagagg cgcatgacat tggtgctggg
     3001 gattgtggtc ggcgtgagca tcgccggcgc gttggccctg agcgccttcc gccagaacgt
     3061 cacgttcttc ttcgacccct cgcaggtcgc cgcaggtcag gtccccgccg gcgagcaatt
     3121 tcggctcgga ggcatggtca ccaagggcag cgtgcagcga accccgggga gtctgcaagt
     3181 acgtttcgtc gtcaccgact tcaagcacaa catccccgtg gtctatgacc gcgtgctgcc
     3241 cgatctcttc agagaagggc agggagtcgt ggcacacggc aggatgcagg ggggcacctt
     3301 cgtcgccgat gaagtgctcg ccaagcacga cgagaagtac atgccgcccg ccgtggcgcg
     3361 ctcgctcaaa cggcctccgc agtcccccaa ccccggaggc gcatgatgct tcctgagctc
     3421 ggtcatttcg tgttgattct ggcgttgctc ttggccgcat tgcaggcctt cttcggcata
     3481 gccggaccgg ccctgcagcg cgatcgctgg attgccgccg tgacaccggc ggtggctggt
     3541 cagttcgtca tgatcgctac cgcggtgatc tgcctcatca gctcattcgt gggcaacgat
     3601 ttctccgtca cctatgtcgc ggaaaattcc aactccgcgc tgccgctctt ctaccgggtg
     3661 acggccatgt ggggcgccca tgaaggctcg ctgctgctgt ggatcttcct tctgagctgc
     3721 tggacggtcg ccgtggcaat cggtgcggcg cgcctgccac cacgattctc ggcgcgcgtt
     3781 ctcggggtgc tgggactcgt gagcttcggg ttcctgctgt tcaccctcgc cacctccaac
     3841 ccgtttctgc gactggatcc gcccgcaccc gacggccgcg atctcaatcc cattctgcag
     3901 gaccccgctc tcgcgattca tcctccgatc ctctacaccg gctatgtcgg gttcgcggtg
     3961 gcgttcgcgt tcgcctgcgc cgcgatgctg gagggccgcc tggaccagct ctgggcgcgc
     4021 tggacacgcc cctggaccac ggcagcgtgg gcctttctca ccgtcgggat ctccctcgga
     4081 agctggtggg cttactatga gctcggctgg ggcgggttct ggttctggga tccggttgag
     4141 aacgcctcgt tcatgccatg gctcgtcggg accgcactca ttcactcgct cgccgtgact
     4201 gacaagcgcg gtctgttcaa gagctggacc ctgctgctcg cgatcctcgc cttctccctg
     4261 agcctgctcg gtacattcct ggttcgctcg ggcgtgctgg tctcggtcca ttcattcgca
     4321 gccgaccccc gccggggggt gttcattctg gcgttcctga tcatcatgat cggcggcgcg
     4381 ctcgggctat acgcctggcg cgcacccaag ctcaaatcgc aagccggttt cgagctgagt
     4441 gcccgtgagt cctttctcct gttcaacaat attctgctgg tgatcgccgc cgccaccgtg
     4501 tttgcgggca cgctggcgcc gctgatcgcg gactccctcg ggctcggcac gctatcggtc
     4561 ggaccgccat atttcgatcc gacgttcatg ctctcgacac tacccttgct cgccctcgtc
     4621 tctctcggca ttcacgcgag ctggaagaag ggccgcttgg gcgacagtgc ccgcactctc
     4681 ctgatgatgc tggtgatcgc attgattgtg ggcgtcatcc tcgcattcgg catctacgcg
     4741 cgcggcaagg tgctcagcct cgtaggagcg acgctcggcg cctggatcat cctgtcctcg
     4801 ttggtcgatc ccgtgcagcg cctgcggcgg gggctctcct tgtcgcggag cgtgctcggc
     4861 atgacggtgg cgcacgtcgg cctcggtgtg ttcgtcatct cgctgacgac ggtcgagacg
     4921 ttcaccgtcg agcgcgacgt cgcaatggcg ccaggacaga gcgccgtagt cgcgaactat
     4981 cagttccgct tcgaggggat ccgggcgatc gaaggtccca attacaacgg ggtggaagcc
     5041 acagtgctgg tcagccgaca tggcaagccc atgacgcggc tttacccgca gaagcgacag
     5101 tactgggtgc agcgctcggc aaccaccgaa gctgcgatcg agatgcatcg cggcagcaat
     5161 ctcttcattg ccttgggtga tgagctcggg gccggcaagt ggagcgtgcg cttccaggtg
     5221 cgacccctgg tcaatttcat ctggctgggc gccttcatca tggcgctcgg cggcgcctgc
     5281 gccgcgacgg atcgccgcta ccggacggcc gcagccaccg cgacgcagac cacggtggcc
     5341 ggctcgcgcg aggaggcggc tgggtgaatc ggtttcttct gccgctcgga gcttttgcgc
     5401 tgctggtgat cgtgctggca gtcgggatca aacgctcgcc tgacaagggc atcattcctt
     5461 cccctctcat cggcaaggcc gcaccgtcct tcagactccc ggtcctcaac gacaccggcc
     5521 gaacattgag caccgccgat ctaaaaggcc gctggtacgt cttcaatgtc tggggcacgt
     5581 ggtgcgtcag ttgccgcg
//
LOCUS       DASRHN010000328        15385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_279016, whole genome shotgun sequence.
ACCESSION   DASRHN010000328 DASRHN010000000
VERSION     DASRHN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15385
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279016"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..380)
                     /locus_tag="VFB37_13055"
     CDS             complement(9..380)
                     /locus_tag="VFB37_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23424.1"
                     /translation="MTTPDQRDTLQSGERRRLSKIVHDHKGTASVVWHDAPTGLERPV
                     FEIEGARQPGKPASRGLNTGSLSIHNEDTFNPYARVPESDQKGGGRPKDLRRLSAWIK
                     LMRQLEESKRKNQDEEDEPST"
     gene            complement(481..1056)
                     /locus_tag="VFB37_13060"
     CDS             complement(481..1056)
                     /locus_tag="VFB37_13060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZO23425.1"
                     /translation="MVYATTSDLMKPEKQTEHFRGRVIRVTTDEVVLPNGHRAQLEVV
                     HHPGGAAVVALDSAQRVCLLHQFRYVGGGWIWELPAGKLEPNEPPLVTAQRELVEEAG
                     TSAREWQSLGTVLSSPGVFSEVLHLFFATGLEPSTVAHEHAEVIEVHWVPLEDAHRWA
                     LDGTIRDAKTVIGLLRAFHIARSASPGTDIP"
     gene            1055..1600
                     /locus_tag="VFB37_13065"
     CDS             1055..1600
                     /locus_tag="VFB37_13065"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO23426.1"
                     /translation="MNKLRFLLITGLPGTGKTTLARELARRYAMALIAKDTIKEALLD
                     VLASGTTGSRELSDAAFAIMLAQAAELLASGVDLILEGNFRAAEHSAKVLATLPRRPP
                     TIVQVLCRLDEPERLARLNARQSDPARHGGHELAAQLGRVPDCDVYLELPGERLAFDA
                     RAGEAGLSQLMASLDGRWSAG"
     gene            1613..4426
                     /locus_tag="VFB37_13070"
     CDS             1613..4426
                     /locus_tag="VFB37_13070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZO23427.1"
                     /translation="MRGGLLIGFVAVWLAACVSTAQPGRTPQAGRPTQPGRASPVGRT
                     VSDLPDLDIPSRRYVLGNGLTLIVHEDHKAPVVAVDVWYHVGSKDERPGRTGFAHLFE
                     HLMFTSSEDHKDEFFRPLLAVGATKMNGNTWLDRTTYFENVPVNALDTALWLESDRMG
                     HLLGAIDQKKLDEQRGVVQNEKRQEESRPYGKVDDLIAAASYPAGHPYSWTAFGSIED
                     LNHASLEDVKDWFRTYYGAANAVLVVAGDVQAEEVRKKVEHYFGDIAPGPLLKHSQSW
                     VAKMSGEKRALLQDRVPQARIYQVWNIPGYATHDFTLLQVAADILAGGKNSRLYKRLV
                     YRDQSATAVSANIGPFEIGSQFQLITTVRPGGDPAVVERAIDEEIARFIKSGPTQEEL
                     ERTRMSLYAGFVRSTERIDGSGGKASILGASQLYAGSPDFYRQSLSWLREASVNDVQD
                     VARSWLSDGVFVLNVEPVPDYHAAGSGADRSKPPIAGPPPPLTLPQMQRATLSNGLHV
                     AVAERHSAPVVQVSLIVDAGHAADSLATPGMSNVTLAMLMEGTRHLDALQIAAHAEAL
                     GAEIGAGSSLDTSYISLNALTSQLEGSLELFADVLLNPTFPRSQLEVLKLQAIAAIEQ
                     QKFQPRGIASRLFPKLLYGAGHAYANPFSGIGTEQSIRSITVERLREFYGRWIRPDNS
                     TLLIVGDTTLTQIVPLLERQLAAWSPPAPAPPTKHLATVPLPQKPRVFLVNRTGAEQS
                     LVMAAQLAPPRADPDNAAFEAINAMLGGNFVSRINMNLREDKHWSYGAGSSLVEAKAQ
                     RPFVVSAMVQTDETAQSMQEILRELREFLGPKPATPEELQFAKNSLVLTLPGSNETAA
                     ELAGSYADILTYGLPDDYLREFSAQVQGLTSAKVHAAATRLIHPESLTWIVVGDLASI
                     EPAIRKLGMGQVQVLDPDGNLLR"
     gene            4501..6591
                     /locus_tag="VFB37_13075"
     CDS             4501..6591
                     /locus_tag="VFB37_13075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZO23428.1"
                     /translation="MPTTSHLEKLNGPQRKAVTYGEPLPEQKGFRSGPLLIVAGAGTG
                     KTDTLAHRVAHLVINGVDPARILMLTFTRRAAIEMRRRAHEIAKKALNDALGGVSQTI
                     TQRLSWAGTFHSIGNRLLRHYAHHLKLDPQFTVVDRADSADMLDGLRQELGYAAKEQR
                     FPRKETCLQIYSYRVNTQKPLKDSLEQQYPWCVQWEADLTRLFRAYVEQKQRASILDY
                     DDLLLYWHVMMSDPRLAQHVGAHFDHVLVDEYQDTNKLQAEILHALRPDGSGLAVVGD
                     DAQAIYSFRAAAVENILGFPDRFRPRAEVVTLAQNYRSTQEVLDVSNALMAEGARQYR
                     KHLLSIRGNGARPRIVTVDELQTQAEYICAEILRRREANVPLKRQAVLFRSSSHSDIL
                     EVELSKRKIPFTKYGGLKFLEAGHIKDVLGVLRWADNPRNTLAAFRIIQILPGMGPAN
                     ARKAVEHLQAHDHALTALRAFNAPPAAQIAFQRLVDLLLTLAEPQRQWQGQVYLVREW
                     YRPHFERLYEHFHTRIGDLDQLELLAGQYPSRERFVTELTLDPPNATSDLAGTPILDE
                     DYLTLTTIHSAKGMEWDTVYLLNVVDGSFPSEFATGRPEMIEEERRLLYVALTRAQNE
                     LILLAPLKFHLTNQSRHGDAHVYGGRSRFLTEKVLKQLDAVSFQGSGLASGAPLLPSA
                     DSTLDVGARLKEMW"
     gene            complement(6619..7449)
                     /locus_tag="VFB37_13080"
     CDS             complement(6619..7449)
                     /locus_tag="VFB37_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23429.1"
                     /translation="MIANRKSSVLLLASMLIAVSCSTTSTRQTTALALDNILNGSQRS
                     AENRARDRYRHPKETLLFFGIRPEMRVLEVWPEPGWYTEILAPLVRERGKYYAAVIEA
                     DPKSKYIAQRLTDYQHKLAERQDLYDRVVVVTFPSQGGDVVPAGSLDMVVTFRNIHNW
                     MARDTATQAFATMYRALRPGGVLGVVEHRGNPSMPQDPRAKSGYVNEDYAIRLIEGQG
                     FKLVGESQINSNPRDTKDYEQGVWTLPPTYRLGDKDRQKYADIGESDRFTLKFIKPKG
                     "
     gene            complement(7600..7881)
                     /locus_tag="VFB37_13085"
     CDS             complement(7600..7881)
                     /locus_tag="VFB37_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZO23430.1"
                     /translation="MTTIYVGNLPFNATEADVKALFERHGKVDSVKLINDRETGKPRG
                     FGFVEMPQNEAQAAIQALNGFQMNGRPLRVNEAQERPQRPRQGGGPFRR"
     gene            8244..8810
                     /locus_tag="VFB37_13090"
     CDS             8244..8810
                     /locus_tag="VFB37_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZO23431.1"
                     /translation="MEAFLISAGVVALAEIGDKTQLFALVLAARFRRPLPIIAGILAA
                     TLANHGAAGAIGTWLSSLLTPTVMRWALILSFVSMAVWILVPDKYEEGGQPPAPRAGV
                     FVSTLVGFFLLEIGDKTQIATIALAAKYHALLPVVAGTTLGMMAVNIPAVLIGHSAAN
                     RLPLKLVRALAAVIFVVLSVLMLFGIGT"
     gene            complement(8905..10959)
                     /gene="speA"
                     /locus_tag="VFB37_13095"
     CDS             complement(8905..10959)
                     /gene="speA"
                     /locus_tag="VFB37_13095"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HZO23432.1"
                     /translation="MRFNVRWLTAHSRESQRVASDPKIALAEGGRKFNPAQPWRIEDS
                     LELYLVNAWGKGYFSINAAGHVVVRPDTQLGHEIDLFDVVEGLKARDLTTPVVVRLSD
                     ILAHRLKNLHAAFAQAIAENDYQNRYAAVFPIKVNQQRLVVEEVYRYGREFGFGLEVG
                     SKPELLAVMAMTENAPDRLIVCNGFKDDSYIEAVTLATKLGRTIIPVVENFDELGLIL
                     KHAATYGVRPRIGVRVKLFSEGSGRWSESAGEKSKFGLFITEIVELFNVLKARDMLDC
                     LQLVHCHPGSQLQDIRRVKDAINELAHVYAELKLMGAGLQYIDVGGGLGVDYDGSGTN
                     YSSSMNYTLNEYASDVVYRVASVCNARDIPHPMIISESGRAVAAYHSVLIFDALGSSA
                     LDKFRVTGNEADDYDGDEELPQPVQDLSVAYRSVSERRLVECYHDALTAREQILQMFN
                     LGLLSLEFRALAERLYWATCARIRDACRKLDSLPEELEDLEAILSDTYFCNFSVFQSL
                     PDSWAIDQLFPIMPIHRLQERPTRTAVLADITCDSDGKIDRFVSLRNVKRTLELHELK
                     EGEKYYMAAFLVGAYQETLGDLHNLFGDTHVVHIRLHDEGGWWIEEIVKGDTANKVLE
                     YMEYDVAELAPALTRDCERAIRDGRMTIAESQVLKRFYDSELDGYAYLEPAAEPVPVT
                     AT"
     gene            complement(11015..11890)
                     /locus_tag="VFB37_13100"
     CDS             complement(11015..11890)
                     /locus_tag="VFB37_13100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZO23433.1"
                     /translation="MHTTLIEPAELAERTDRPDWTVLDCRFDLARPQWGEQAYAAGHI
                     PHALYAHLDRDLSGPITSHSGRHPLPDISSLAATLGRWGIDASVQVIAYDQGNGAYAA
                     RLWWLLRWLGHPAVAVLNGGFAAWQQQSLPVSTQTDARQARQFVPHAADSEVISTAAL
                     AEWVRAGELARGARTLVDARGADRFAGQNETLDSIAGHVPGAHNHPFARNLDAQGHFL
                     PPEELRARWLGTLAGRSPSQVVSMCGSGVTACHNLLALEVAGLRGARLYAGSWSEWIR
                     DASRPVEPPSTSDNA"
     gene            11966..12640
                     /locus_tag="VFB37_13105"
     CDS             11966..12640
                     /locus_tag="VFB37_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZO23434.1"
                     /translation="MSESTMAIDVYWGSGSPYSWRVLLALEYKRLAYTSHVLQFSKQE
                     HKSPQMLALNWRGKVPVLKDRDYVCFESLAVLYYLDLKYPEPPIFGRTPEEAGTIMRV
                     ICEYQAYAEPHVGAIVTAVFGKADLGADAVTEAMHHAASEARTIEARLSKSDWVVGET
                     FSAADMVIFPGFQLLRRALERPEARELASRFLPVEVNYPALSRWVARVEALPGYDRTY
                     PPHWRR"
     gene            12827..14248
                     /locus_tag="VFB37_13110"
     CDS             12827..14248
                     /locus_tag="VFB37_13110"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZO23435.1"
                     /translation="MSNHKVYVDFPGRILMVGFGSIGQGVLPLLLRHIGISPDRLTIV
                     AADDKGRAVAAQYGVKLIKETLVRENFRRILTPLLGRGDFLLNVSVEVSSVALVKFCW
                     ERGAMYLDTCIEPWPGGYTDPTVAAAKRTNYALREEALALRSGDDRAPTAVLTHGANP
                     GLVSHLLKQALLNVAADTKVDAGDPKSRAEWGQLARQLGVKVIHIAERDTQVSNVPKE
                     PGEFVNTWSIDGFVSEGSQPSEMGWGTHERNFPRDGKRHDFGCMCAIYLMQPGAGTRV
                     RTWTPKAGYFHGLCITHGEAISIADYYTVKEGSKLAYRPTVHYAYHPCDSAVMSVHEL
                     LGRNYVQQERQRILMDEIVKGIDELGVLLAGHKKNAYWYGSQLSIDQARKLAPHNNAT
                     SLQVTSAVLAGVIWAMENPNCGIVEPDEMDFRRNLEIALPYLGPVVGQYTDWTPLHER
                     ERLFAEDLDKTDPWQFKNVRVLW"
     gene            complement(14223..14747)
                     /gene="greB"
                     /locus_tag="VFB37_13115"
     CDS             complement(14223..14747)
                     /gene="greB"
                     /locus_tag="VFB37_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HZO23436.1"
                     /translation="MGRFRPPQPPASQYITPEGARRLREELDQLWRQERPRVTQAVAE
                     AAAQGDRSENAEYTYGKRRLREIDRRVRFLRMRLEGMVIVDQPPSDPGRVFFGAWVVL
                     ESEDGSQSRYRIVGPDEFDLAPGYISMDSPLGRALLRKALDEEVTIELPDGRRSYVVV
                     EIEYPSTTTAPGRS"
     gene            14844..15050
                     /locus_tag="VFB37_13120"
     CDS             14844..15050
                     /locus_tag="VFB37_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23437.1"
                     /translation="MATKYYTHFVTARARAQAVANANEWSGVVELRHPLENLGGTREL
                     RALLAQSFDLDCEDIKILHWARLH"
     gene            complement(15198..>15385)
                     /locus_tag="VFB37_13125"
     CDS             complement(15198..>15385)
                     /locus_tag="VFB37_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23438.1"
                     /translation="TAEQLTFLRSLGCDQYQGYHCSPPLPPTEFLTLLPHRSLDESKR
                     VPASLEDTMISRVLRNG"
BASE COUNT         2750 a         4973 c         4926 g         2736 t
ORIGIN      
        1 cgcaccgctt aggtgctggg ctcgtcctct tcgtcctgat tcttgcgctt gctctcttcg
       61 agctgccgca tcagcttgat ccaggccgac agacggcgca ggtccttggg gcgtccgccc
      121 cccttctgat ccgactccgg aacgcgcgcg tagggattga aagtgtcttc gttgtggatc
      181 gacagcgagc ccgtgttcag gccgcgggag gcaggcttgc cgggctggcg tgcgccttcg
      241 atctcgaaca ccggacgctc gaggccggtg ggagcatcgt gccacacgac cgaggcagtt
      301 cccttgtgat cgtgcacgat cttactcagg cgcctgcgtt ctccggactg caaggtatcg
      361 cgctgatctg gcgtggtcac tcgacgttta cggtcatcca tggaataagt ttgatcgagc
      421 cgggccccat ccggcacacg tacggtacct gccgtcccgc ctccggttcc gagagatccg
      481 tcacggaatg tctgtccccg gcgacgcgct ccgagctatg tgaaatgccc gcaacaaccc
      541 gatcaccgtc ttcgcatcgc ggatcgtgcc gtcgagcgcc cagcggtgcg catcctcgag
      601 gggcacccag tgaacttcga tcacttccgc atgctcatgc gccacggtgc tcggctccag
      661 gccggtggcg aagaacaggt gcaacacctc gctgaatacc cctggtgagc tcagaaccgt
      721 acccagggac tgccactcgc gcgcactggt gccagcctcc tcgaccagct cgcgttgcgc
      781 ggtcaccagc ggcggctcgt tcggctcgag cttgcccgcg ggcagctccc agatccagcc
      841 gccgccaaca tagcggaact ggtgcaggag acacacgcgc tgcgctgaat ccaacgccac
      901 cacggccgcg ccgcccggat ggtggaccac ctcgagctgg gcacgatgtc cgttcgggag
      961 caccacctca tccgtcgtca cacggatcac gcggccacgg aagtgctcgg tctgcttttc
     1021 gggtttcatg aggtcggaag ttgtagcata gaccatgaac aaactccgct ttctcctcat
     1081 cacgggtctg ccgggcacgg gcaagacgac gctcgcgcga gagctcgcgc gccgctacgc
     1141 gatggcactc atcgccaagg acacgatcaa ggaagcgttg ctcgatgtgt tggcgagcgg
     1201 caccaccggc tcgcgcgagt tgagcgatgc cgctttcgcc atcatgctcg cccaggcggc
     1261 ggagcttctg gcgagcggag tcgacctgat tctcgagggc aatttccgcg ctgcagagca
     1321 ctccgcgaaa gtactggcca cgctcccccg ccgaccgccg acaatcgttc aggtgctctg
     1381 tcggctcgac gaaccggagc gtctggcgcg gctcaatgcg cgacaatccg acccggcccg
     1441 ccatggcgga catgagctgg cggcgcagct cgggcgcgtt cccgattgcg atgtctatct
     1501 cgagctgccc ggtgaacggc tggcgttcga tgctcgtgcg ggcgaggcag gcttgagtca
     1561 actgatggca tcattggacg gtcgatggtc ggcgggatga aggaggcggg acttgcgcgg
     1621 tggattgctg atcggctttg tcgccgtgtg gctcgctgca tgcgtctcga cggcccagcc
     1681 cggacgcacg ccccaggctg gacggcccac ccagcccgga cgggcatccc cggtcggccg
     1741 gacagtcagc gatttacctg acctcgatat tcccagtcgc aggtacgttc ttggcaacgg
     1801 cctgacgctg atcgtgcacg aggatcacaa ggcgccggtc gttgcggtag acgtgtggta
     1861 tcacgtcgga agcaaggacg aacgcccggg gcgcaccggc tttgcgcatc tgttcgagca
     1921 cctgatgttc acgagctcgg aagaccacaa agatgagttc ttccgtccat tgctcgctgt
     1981 cggtgcaacg aagatgaatg gcaatacgtg gctcgatcgg accacctact tcgagaacgt
     2041 gccggtcaat gctctggata cggcgttgtg gctggagtcc gaccgcatgg gacacctgct
     2101 gggggccatc gatcagaaga agctcgatga gcagcgcggc gtggtgcaga acgagaagcg
     2161 ccaggaagag agccggccct acggcaaggt cgatgatctg atcgccgccg ccagctatcc
     2221 tgccggccac ccgtattcct ggactgcgtt cggctcgatc gaggatctga atcacgcctc
     2281 gctggaggat gtgaaggatt ggttccggac ctactacggg gccgcgaacg ccgtgctggt
     2341 agtcgcaggc gatgtgcagg ccgaggaggt ccgcaagaag gtcgagcact acttcggcga
     2401 catcgcgccg ggccccctgc tcaaacactc gcagagctgg gtcgcgaaga tgagcggcga
     2461 gaagcgcgcg ctgctccagg atcgagtgcc gcaggcgagg atctaccagg tctggaacat
     2521 acctggctac gccacgcatg acttcacgct gctgcaggtg gcggccgata ttctcgcggg
     2581 tggcaagaac agccgcctgt acaagcggct ggtgtaccgg gaccagagcg ccacggcggt
     2641 gagcgccaac atcggcccgt tcgagatcgg ctcgcagttc cagctcatca cgactgtccg
     2701 acccggtggg gatcccgcgg tcgtcgagcg ggccattgac gaggagatcg cgcgcttcat
     2761 caagagcggt cccacccagg aagagctcga gcggacccgg atgtcgcttt atgcgggctt
     2821 cgtacgcagc acggagcgaa tcgacggctc aggcggcaag gcctcgatcc tcggcgcaag
     2881 tcagctgtac gccggctcac ccgacttcta caggcagtcc ttgagctggc tgcgggaggc
     2941 gagcgtcaac gatgtgcagg atgtcgcccg ctcctggctg tccgatggcg tgttcgtcct
     3001 caatgtcgag ccggtacccg actatcatgc ggccggcagt ggtgcggatc gcagcaagcc
     3061 gcccatagcc ggtcctccgc ccccgctcac tctgccccag atgcagcgcg ctacgctctc
     3121 caatggattg catgttgcgg tcgcggagcg acattcggcc ccggtcgtcc aggtgagcct
     3181 gatcgtcgat gccggacatg cggcggacag tctggccacg ccgggcatgt cgaatgtcac
     3241 cctggcgatg ctcatggaag gcacccggca tctggatgcg ctgcagatcg cagcgcatgc
     3301 cgaggcgctg ggagcggaga tcggcgcggg ctcgagtctc gacacctcct acatctcgct
     3361 gaatgcgctc acgagtcagc tcgaaggctc cctcgagctg ttcgccgatg tgctgctcaa
     3421 tcccaccttc ccgcgcagcc agctcgaggt gctgaagctc caggcgatcg cagcgatcga
     3481 gcagcagaag tttcagccgc gcggaatcgc ctcgcggctg tttccgaagc tgctgtacgg
     3541 agccgggcat gcctacgcga accctttctc cgggatcggt acggagcaga gcattcgctc
     3601 gatcaccgtc gagcgacttc gcgagttcta tgggcgctgg atccggccgg acaactcgac
     3661 gctcctcatc gtcggcgaca cgacgctgac ccagatcgtc ccgctgctcg aaaggcagct
     3721 cgccgcctgg tctcccccgg cgccagctcc gcccaccaaa cacctggcca ccgtcccgct
     3781 cccgcagaaa ccccgcgtgt tcctcgtcaa ccgcaccggc gccgagcagt cgctggtgat
     3841 ggccgcgcag ctcgcgccac cgcgcgcgga cccggacaac gcagctttcg aggcgatcaa
     3901 cgccatgctg gggggcaact tcgtctcgcg gatcaacatg aacctgcgcg aggacaagca
     3961 ctggtcctac ggcgccggta gcagcctggt cgaggccaag gcgcagcggc ccttcgtggt
     4021 gagcgcgatg gtgcagaccg atgagacggc gcagtcgatg caggagatct tgagagagct
     4081 gcgcgagttc ctcggtccga agccggcgac cccggaagag cttcagttcg ccaagaacag
     4141 cctggtgctg accctgccgg gcagcaacga aacggcagcc gagctggccg gatcctacgc
     4201 agacatcctc acctatgggc tgcccgatga ttacctgaga gagttttccg cccaggtgca
     4261 gggcctgacg agcgccaagg tgcacgccgc ggcgacccga ctcatccacc ccgagtcact
     4321 cacctggatc gtcgtgggtg atctggcgag catcgagccg gccatccgca agctgggaat
     4381 gggacaggtc caggtgctcg atcccgacgg caatctgctg cgctgagtcg aggaaattat
     4441 tcaaggcgtg actgtatgca gtgacaggcg ggcagtgcgt tcgtatattc gacgctgcga
     4501 atgccaacca cgtcacacct cgaaaagttg aacgggccac agcgcaaggc ggtgacctac
     4561 ggcgagccgt tgccggaaca gaaaggattc cggtccggtc cactgctgat cgtcgcgggc
     4621 gcaggcaccg gcaagaccga cacactggcc catcgcgttg cgcacctggt catcaacggt
     4681 gtggacccgg cgcgcatcct catgctgacg ttcacgcgac gagcggccat cgagatgcgc
     4741 cgccgcgcgc acgagatcgc caagaaggcc ctcaatgatg cgctgggcgg cgtgagtcag
     4801 acgatcacac agcggctgag ctgggcaggc acctttcact cgatcggcaa ccgcctgctg
     4861 cgccactatg cacaccatct gaagctcgac ccgcaattta cggtggtgga tcgggcggac
     4921 tcggccgata tgctggatgg cctgcgtcag gagctcgggt atgcggccaa ggagcagcgc
     4981 ttcccccgca aggaaacctg cctgcagatc tactcgtacc gggtcaacac gcagaagccg
     5041 ctgaaggaca gcctcgagca gcaatacccc tggtgcgtgc agtgggaagc ggacctgacc
     5101 cgcctgttcc gcgcttacgt ggagcagaag cagcgcgcca gtattctgga ttacgacgat
     5161 ctgctgctct actggcacgt catgatgtcg gatccgcgcc tcgcgcagca cgtcggcgcg
     5221 cactttgacc acgtcctggt cgatgaatac caggatacga acaagctgca ggcggagatc
     5281 ctgcacgcgc tgcgccccga tggctcgggc ctcgcggtgg tcggggatga cgcgcaggcg
     5341 atctattcct tccgcgctgc cgcggtcgag aacattctcg ggttccccga tcgcttcagg
     5401 ccgcgtgctg aagtggtcac cctcgcgcag aactaccgct cgacacagga agtgctcgat
     5461 gtctccaacg cgctgatggc ggaaggcgcg cgccagtacc gcaagcacct gctctccatc
     5521 cggggcaacg gcgcgcgtcc gcgcatcgtc acggtcgatg agctgcagac ccaggccgag
     5581 tacatctgcg cggaaatcct gcgccgccgc gaggcgaacg tgcccttgaa gcgccaggcc
     5641 gtgctgttcc gcagctcgag ccacagcgac atcctggaag tggagctcag caagcgcaaa
     5701 atccccttca ccaaatacgg tggcctcaag ttcctcgagg ccgggcacat caaggacgtg
     5761 ctcggggtgc tgcgctgggc ggacaatccg cgcaataccc tggcggcctt ccgcatcatt
     5821 cagattctgc ccggcatggg cccggccaat gcccgcaagg cggtcgagca tctgcaggcc
     5881 cacgatcatg cgctcacggc gctgcgggcc ttcaacgcgc cgcccgcggc gcagattgcc
     5941 tttcagaggc tggtcgatct gctgctgacg ctcgccgagc cgcaacgcca gtggcaaggc
     6001 caggtgtatc tcgtgcgcga gtggtaccgg ccgcatttcg agcggctgta cgagcatttc
     6061 cacacccgca tcggagatct ggatcagctc gagctgctcg ccgggcagta tccctcccgc
     6121 gagcgcttcg tcaccgagct cacgctcgat ccgcccaatg ccacgagtga tctggccggc
     6181 accccaattc tcgatgagga ctatctgacc ctcaccacga tccattccgc caagggaatg
     6241 gagtgggaca cggtctatct actgaacgtc gtggacggca gtttcccctc ggagttcgcc
     6301 accggcaggc ccgagatgat cgaggaggag cgccggctgc tgtatgtggc gctgactcgc
     6361 gcccagaacg agctgatcct gctcgccccg ttgaagtttc atctcaccaa ccagtcgcgc
     6421 cacggggatg ctcacgtata cggcgggcgc agccgcttcc tcaccgagaa ggtcctgaag
     6481 cagctcgatg cggtgagctt tcagggcagc ggcctcgcca gcggcgcgcc gttgctgccg
     6541 agtgccgact ccacgctcga tgtgggcgcc cgcctcaagg aaatgtggtg agcccctgcc
     6601 ggcggcggcc ggcgcacatc agcccttggg cttgatgaac ttcagcgtga aacggtcgct
     6661 ttcgccaatg tcggcgtact tctgccggtc cttgtcaccc agccgatagg tcggaggcag
     6721 agtccagacc ccctgctcgt aatccttggt gtcgcgcggg ttggagttga tctgcgattc
     6781 gcccacgagc ttgaaaccct gtccctcgat cagccggatc gcataatcct cgttcacata
     6841 gccgctcttg gcgcgcggat cctgcggcat ggacgggttg ccccgatgct ccacgacacc
     6901 gagcacgccc ccgggcctga gagccctgta catcgtggca aaggcctgcg tcgccgtatc
     6961 gcgtgccatc cagttatgga tgttacgaaa tgtcaccacc atatcgagac tacccgccgg
     7021 cacgacatcg ccgccctgcg acgggaaggt gaccacgacg acccggtcat acaggtcctg
     7081 gcgttcggcc agcttgtgtt ggtaatccgt cagacgctgc gcaatgtact tgctcttcgg
     7141 atccgcctcg atgacggcgg cgtagtactt gccgcgttcc cgcaccagcg gtgcaaggat
     7201 ttccgtgtac catccgggct cgggccagac ctccagcacg cgcatttccg ggcgtatccc
     7261 gaagaaaagc agcgtctcct tcggatgacg atagcggtcg cgggcgcggt tctcggccga
     7321 gcgctggctg ccgttgagga tgttgtcgag tgccagagcg gtcgtctgcc gggtgctcgt
     7381 ggtcgagcag ctcaccgcga tcagcatcga ggcgagcagc aggacggagg actttcggtt
     7441 cgcgatcatg aagacatccg caggcatgga cggggcgtgg cgcagcctac tacaggcgca
     7501 cggaaagcat gaacggtccc ggctcgcaca caaaaaaccc cgcctgggag cggggtttcc
     7561 tgatagcgcg tgcggtgcgt gaggcccggt gagagggaat cagcggcgga atggacctcc
     7621 gccctggcgc gggcgctgcg ggcgctcctg ggcttcgttc acccgcaggg gccgcccgtt
     7681 catctggaag ccgttgaggg cctggatcgc agcctgcgcc tcgttctggg gcatctcgac
     7741 gaacccgaag ccgcgcggct tgccggtctc acggtcattg atcagcttca ccgaatccac
     7801 cttgccgtga cgctcgaaaa gagctttgac gtccgcctcg gtggcattga agggcagatt
     7861 gcccacatag atcgtcgtca tccggaggtc tcttttggga aagcatacag cggcgagccg
     7921 cacggggcgg tcgcatgtac acgtgatcgg tagtcgaaca ggtcgaggct acaacgcagc
     7981 acggtgagct tttgttatga ggtcaatgcg cagcgcttgc ggcctgacgg cctgcacctt
     8041 cgttcgtgta cgggggagcc gtggcctgga agggtaagat aacaatcaac atggtcgcgc
     8101 ggactcatcc gtggcggaca cgaacgccac cggatgctac tcccggcatt tgcgggaagc
     8161 aaacatctgg cggcgccaca cccggggctc tcgcggcatc gcgtcccgag ctcggtagca
     8221 cctgagggac tggaagcaac tccttggaag ccttcctcat ttcagccggc gtggtggcac
     8281 ttgccgagat cggcgacaag acccaactgt tcgcgctggt gctggccgcc cgcttccggc
     8341 gccccctgcc gatcatcgcc ggaattctcg ccgcgaccct ggcgaaccac ggcgcggccg
     8401 gcgccatcgg gacctggctg agctccctgc tgacacccac cgtgatgcgc tgggcactga
     8461 tcctctcgtt cgtctcgatg gcggtctgga tcctggtccc ggacaagtac gaggaaggcg
     8521 gccagccccc ggcgccgcgc gccggggtct ttgtcagcac gctcgtgggg ttcttcctgc
     8581 tcgagatcgg ggacaagact cagatcgcca ccatcgccct tgcggcgaag tatcacgctc
     8641 tgctgccggt cgtggccggg acgaccctcg gcatgatggc cgtgaacatt ccggccgtgc
     8701 tcatcggcca ctcggcggcg aaccgcctgc ccttgaagct ggttcgagcg cttgcggcag
     8761 tgatcttcgt ggtgctgagc gtgctcatgc tgtttggaat cggtacatga gagagcccct
     8821 ggcgggacgc gaggggctgg cccgcgtggt ctcgccaggc cgcgcgggcg agaggtcgcc
     8881 tgagtgcgcg gccgaggcgg ggcgtcaggt cgccgtaacc gggaccggct cggcggcggg
     8941 ctcgaggtag gcgtatccgt cgagctcgct gtcgtagaag cgcttgagca cctggctctc
     9001 cgcgatggtc atccgtccat cgcgaatcgc gcgctcgcaa tcgcgagtca gggccggcgc
     9061 aagctccgcg acgtcatact ccatgtactc gaggaccttg ttggccgtat cacccttgac
     9121 gatctcctcg atccaccagc cgccctcatc gtgcaggcgg atgtgcacca catgggtgtc
     9181 gccgaacaga ttgtgcagat cgccgagagt ttcctggtag gcaccgacga gaaaggccgc
     9241 catgtagtac ttctcgcctt ccttgagctc gtgcagttcc agcgtgcgct tcacgttgcg
     9301 aagcgagacg aagcggtcga tcttgccgtc ggagtcgcag gtgatatccg ccagaacggc
     9361 ggtgcgggtc ggccgctcct gcaggcgatg gatcggcatg atgggaaaga gctgatcgat
     9421 ggcccagctg tccggcagcg actgaaagac cgagaagttg cagaagtagg tatccgaaag
     9481 gatggcctcg agatcctcga gctcctcggg caggctgtcg agcttgcggc aggcatcgcg
     9541 gatccgggcg caggtggccc agtacagccg ctcggcaagc gcgcggaatt ccagcgacag
     9601 cagcccaagg ttgaacatct gcagaatctg ctcgcgcgcc gtgagggcat cgtgatagca
     9661 ctccaccagg cgccgctcgc tcacggaccg ataggcgacc gacaggtcct gcaccggttg
     9721 tggcagctcc tcgtccccgt cgtagtcgtc ggcctcattg cccgtcacgc ggaatttgtc
     9781 gagtgccgag gagccgaggg catcgaagat caacacgctg tgataggccg caaccgcgcg
     9841 cccggactca ctgatgatca tcgggtgcgg aatgtctcgc gcattgcaga cgctcgcgac
     9901 ccgataaacg acatcgcttg cgtactcgtt gagcgtgtaa ttcatcgagg aggaatagtt
     9961 ggttccactg ccgtcatagt cgaccccgag tccgccgccc acatcgatgt actgcagtcc
    10021 cgcgcccatg agcttcaatt cggcatacac gtgcgcgagc tcgttgatcg catccttcac
    10081 gcggcggatg tcctggagct gactgcccgg atggcagtgc acgagctgca ggcagtcgag
    10141 catgtcgcgt gccttgagga cgttgaacag ctcgacgatc tcggtgatga acagcccgaa
    10201 cttggacttc tcacccgctg actcgctcca gcgtcccgag ccctcactga agagcttcac
    10261 gcgcacaccg atgcgcggcc gcacaccgta ggtcgcggcg tgcttgagga tgagccccag
    10321 ctcgtcgaaa ttctccacga ccgggatgat cgtccgtccg agcttcgtcg cgagagtcac
    10381 ggcctcgatg tagctgtcgt ccttgaaacc gttgcacacg atcagtcggt cgggggcgtt
    10441 ctcggtcatg gccatgacgg ccagcagctc gggcttggag ccgacttcga gcccgaagcc
    10501 gaactcgcgt ccgtaccggt aaacctcctc caccacgagc cgctgctgat tcaccttgat
    10561 cgggaacacg gcggcatagc ggttctggta atcgttctcc gcgatggcct gcgcgaaggc
    10621 ggcgtgcaga ttcttcagcc gatgcgccag gatatccgac agccgcacga ccaccggtgt
    10681 cgtcagatcg cgggccttga gtccttcgac cacatcgaac agatcgatct cgtggccgag
    10741 ctgggtgtcc gggcggacca ccacgtggcc ggccgcgttg atcgagaagt agcccttgcc
    10801 ccaggcgttc accagataga gctcgaggga gtcctcgatg cgccagggct gggcggggtt
    10861 gaacttgcgg ccgccctcag cgagggcaat cttcggatcg gaagccacgc gttgtgattc
    10921 ccttgaatgc gcggtgagcc aacggacgtt gaaacgcacg atagcaaaat tgcatgacga
    10981 ggaagagtcg tctgcggcgc atttctgaca ccgctcatgc gttgtccgac gtgctcggcg
    11041 gctcgaccgg gcggctcgca tcgcgaatcc actcgctcca ggagccggcg taaagacggg
    11101 cgccgcgcaa gcccgccact tccagcgcga gcaggttgtg gcacgccgtc acgcccgagc
    11161 cgcacatgga aaccacctgc gagggcgacc gtccggcgag ggtgccgagc cagcgggctc
    11221 gaagctcttc gggcggcagg aaatggcctt gcgcgtcgag attgcgcgcg aagggatgat
    11281 tgtgggcgcc gggcacgtgg cccgcgatcg aatccagtgt ttcattctga cccgcgaacc
    11341 ggtctgcacc gcgggcatcg accaatgtgc gcgcgcctcg ggcaagctca ccagcgcgca
    11401 cccattccgc gagtgctgcg gtggaaatga cttccgagtc ggcggcatgc ggcacgaact
    11461 gacgagcctg cctggcatcg gtttgcgtgc tcactggcag ggattgttgt tgccacgcgg
    11521 cgaagccgcc gttcaggacc gcgacggccg ggtggcccaa ccagcgcaac agccaccaca
    11581 gccgcgccgc gtatgcgcca ttgccctggt cataggcgat cacctgcaca cttgcatcga
    11641 ttccccagcg tccgagcgtt gccgcaaggg agctgatgtc cggcagagga tggcggccgc
    11701 tgtgcgaggt gatcgggccg gacaggtcgc gatcgaggtg agcgtaaagg gcatgcggga
    11761 tgtggccggc tgcataggcc tgctcgcccc attgcgggcg cgcgaggtcg aatctgcaat
    11821 ccaggacagt ccaatccggg cggtcggtcc gctcggccag ctccgcgggc tcgatcagag
    11881 tcgtgtgcat gagcgcctcc ggcatggctt cgtcacatat gcattttctg cggtgtccgc
    11941 ttctcgttac aatcccggga agatcttgag cgagagcacg atggcgatcg acgtttactg
    12001 gggaagcggc agtccttact cgtggcgggt gctgctcgcg ctcgagtaca agcgcctggc
    12061 gtacacgagc cacgtcctgc agttctccaa gcaggagcac aagtccccgc agatgctcgc
    12121 cctcaactgg cgcggcaaag ttcccgttct caaggaccgc gactacgtct gcttcgagtc
    12181 actcgcggtc ctgtactacc tcgatctgaa gtatcccgag cctccgattt ttggccgtac
    12241 accggaggaa gctggcacca tcatgcgggt catctgcgag taccaggcgt acgccgagcc
    12301 gcatgtcggg gcgatcgtga ctgccgtttt cggcaaggct gacctgggcg cggatgctgt
    12361 gaccgaggcg atgcatcacg cggccagcga ggctcgcacc atcgaggcac ggctttcgaa
    12421 gtccgactgg gtggtgggcg aaaccttctc tgcggccgac atggtcattt tcccgggctt
    12481 ccagttgttg cgccgcgctc tggaaagacc cgaggcgcgc gagctggcct cgcgcttcct
    12541 gccggtcgag gtcaactacc cggccctctc gcgctgggtc gcgcgggtcg aggcccttcc
    12601 cggttacgat cggacctatc cgccgcactg gcggcgctga gcccgagcgc gggcttccga
    12661 ccggactacg aagcgatgtc cgcgatgtcc gctgaaattg ccgcagggtg atttcagcca
    12721 atgagttgag gcagcaggcg aaagccacgc ctttcgcctg cggactacga agcgatgtcc
    12781 gctgaaattg ccgcaggcaa tttcagccaa tgaaactagg agcatcatga gcaatcacaa
    12841 ggtgtacgtc gatttcccgg gccggatcct gatggtgggc ttcggcagta tcgggcaagg
    12901 cgtgctgccg ctgctcctgc ggcatatcgg aatctcaccc gatcggctga cgatcgtcgc
    12961 ggccgacgac aagggacgcg ccgttgcggc ccagtacggt gtcaagctca tcaaggagac
    13021 cctggtccgg gagaatttcc ggcgcatcct gacaccgctg ctcggccgcg gagactttct
    13081 gctcaatgtc tccgtcgaag tctccagcgt ggctctagtc aagttctgct gggagcgcgg
    13141 tgccatgtat ctcgacacat gcatcgagcc ctggccgggc gggtacacgg atccaacggt
    13201 cgcagcggcc aagcgtacga actacgccct ccgggaagag gcactcgcgc tgcgctccgg
    13261 cgacgatcgc gcgccgaccg cggtgctcac tcacggagcc aaccctggcc tcgtctcgca
    13321 tctgctcaag caggcgctcc tcaacgtcgc cgccgacacc aaggtggatg ccggcgatcc
    13381 caaatcgcgc gccgagtggg gtcagctcgc gcggcagctc ggggtcaagg tcattcacat
    13441 cgccgagcgc gatacccagg tgagtaacgt tcccaaggag cccggagagt tcgtcaacac
    13501 ctggtcgatc gatgggttcg tcagcgaagg ctcgcaaccg agtgagatgg gatgggggac
    13561 ccacgaacgc aatttcccac gcgatggcaa gcgtcacgac ttcggttgca tgtgcgccat
    13621 ctatctcatg cagccggggg caggcacgcg cgtgcggacc tggacaccga aagccggcta
    13681 cttccatggg ctgtgcatca cccacggcga ggccatctcg atagccgatt actacaccgt
    13741 gaaagaaggc tccaagctcg cctaccggcc cacggtgcac tacgcctacc acccttgcga
    13801 ttccgccgtg atgtccgtgc acgagctgct cgggcgcaac tacgtgcagc aggagcgcca
    13861 gcgcatcctc atggatgaga tcgtcaaggg aatcgatgag ctcggcgtgc tcctcgccgg
    13921 tcacaagaag aacgcctact ggtacggatc ccagctgtcc atcgaccagg cgcgcaagct
    13981 cgcaccccac aacaatgcga ccagcctcca ggtcacatcg gccgtgctcg ccggcgtcat
    14041 ctgggccatg gagaatccca actgcggcat cgtcgagccc gacgagatgg atttccggcg
    14101 caacctcgag atcgcgctgc cgtatctggg accggtggtc gggcagtaca ccgattggac
    14161 gccgctgcac gagcgcgagc ggcttttcgc cgaggacctg gacaagaccg acccctggca
    14221 gttcaagaac gtccgggtgc tgtggtagtc gaggggtact cgatttcgac aacgacgtat
    14281 gaacggcggc cgtccggcag ctctatggtg acttcctcgt cgagcgcttt gcgcaggagc
    14341 gcgcggccga gcggggaatc catgctgatg taacccgggg ccaggtcgaa ctcgtccggc
    14401 cccacgatcc ggtaacgcga ctggctgcca tcctcggatt cgaggacgac ccaggcaccg
    14461 aagaagacgc gccccggatc cgaagggggt tgatcgacga tgaccatgcc ctcgagccgc
    14521 atgcgcagga agcgcacgcg gcggtcgatc tcccgcagcc ggcgcttgcc ataggtgtat
    14581 tccgcatttt cggaccggtc gccctgcgcg gccgcctcgg cgaccgcctg agtcacccgc
    14641 gggcgttcct gccgccacag ctgatccagc tcctcacgca gccggcgggc cccttcgggc
    14701 gtgatgtatt gggaggccgg cggctgaggg gggcggaatc gtcccattgg gattatgtcg
    14761 agattccttt gacaaaaatc gaaggtgttc tgtgggccaa ttcacggacg atcgcgctgc
    14821 acgggcacta atctcattcc gcaatggcaa ccaagtacta cacccacttc gtcactgcgc
    14881 gagcccgggc acaggctgtg gccaacgcga acgagtggag cggggtagtc gagctgagac
    14941 atccgctcga gaacctcggt ggtacacggg agctgcgggc gctgcttgcg cagagtttcg
    15001 acctcgactg cgaggacatc aagatcctgc actgggcccg gttgcactga tatcccctag
    15061 cggacttcct tcccttcatc gaaagtccgc cttctgaccc caggcttacg cctggggttt
    15121 tttttgattg gcgccaaacg ccggaggcgt ttggcgcagg ccaagcccta ttgggcagcc
    15181 cgcacgcggg ccggtgacta gccgttgcgc agcacccggc tgatcatcgt gtcctcgagg
    15241 ctggccggca cacgcttgga ctcatccagg ctgcgatgcg gcagcagggt caggaactcg
    15301 gtcgggggca gcggcgggct gcagtgatag ccctggtact gatcgcagcc gagcgagcgc
    15361 aggaaggtga gttgctcggc cgtct
//
LOCUS       DASRHN010000329         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1273729, whole genome shotgun sequence.
ACCESSION   DASRHN010000329 DASRHN010000000
VERSION     DASRHN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273729"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFB37_13130"
     CDS             complement(<1..315)
                     /locus_tag="VFB37_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZO23439.1"
                     /translation="MGTYLRDQTVERYTRVAIILHWLIALGLVGQIAFGWFLETVPRG
                     TPPRGFYVNLHKSTGLTLALLILARLGWRIANRPPRLPVLMPAWERVAARWSHVALYA
                     CML"
     gene            541..1122
                     /locus_tag="VFB37_13135"
     CDS             541..1122
                     /locus_tag="VFB37_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23440.1"
                     /translation="MLLNPTQFSQQGLMPQFAGQQAGPPQMGAGLFGQPGAYGANGYP
                     PGAQPYPFAGQGNPFLQSSLTQSPFAQNPYLSGFGGAQHPAQQIVQILGQLVQHIFTQ
                     SALTQQIGIAIQQLAQITVQSLQSQQGQLGGQLGGQLGGQLGGQSFGLGGGIGSQGFG
                     GGGQPFGQSPFGAQGGFGQQPQPWGLNRPPTIQ"
     gene            1244..>2169
                     /locus_tag="VFB37_13140"
     CDS             1244..>2169
                     /locus_tag="VFB37_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23441.1"
                     /translation="MAPEKEERPTAAAARKAGEAGEDSARKTAGSQSRTPRESGNGGN
                     GGLTAALPPTRRERYIIGTRTTGVQPFGQPQQQHSMNDVVEYLKHQENVEVVERIKLG
                     GTEPFTADGRNVSEAVVAKIDPGKAQRLQTIAPPDLVIERDSSLACADYLAMPARVAP
                     IGTLLPLRSVATEVVIRVIGERDQPLARATVVIDGGGLPAQALTDDNGTARVTFFGGS
                     IETIQTLFVRSAANHWDRVIRAPRLNPGSNTVRLRPLSDFYPNFPSERVLSWGQRLLG
                     LDPASGRFSGSGVRIGIIDSGCDNSHPLLRHV"
BASE COUNT          415 a          731 c          680 g          343 t
ORIGIN      
        1 gagcatgcac gcgtacaggg ccacatgact ccatcgcgcc gcaacgcgct cccacgcggg
       61 catgagcacc ggcagccgcg gcgggcgatt cgcgatgcgc catccgagcc tcgcgaggat
      121 gagcagcgcc agcgtcaatc ccgtggactt gtgcagattc acatagaagc cacgcggtgg
      181 cgtgccgcgc ggaaccgtct cgagaaacca accaaacgcg atttgaccca ccagcccaag
      241 cgcgatcagc cagtgcagga tgatggccac cctcgtgtaa cgctcgacag tctgatcgcg
      301 caaatacgtg cccatactcc cgccctgctg cgcggttcgc gagggcggat cgcgtaagca
      361 agcgctgcgc ccgccgagct ccgggtcgtc gtgggcgggc gccatagccc gtctttgtac
      421 cctcactggg attttcggac tattcccgtg ggtgcgcccg cggccgatga ttttaccgcg
      481 atcgacgacc agtcatatcg gcgacgcccg aagcttttcc ttcacacgga gaaacccaca
      541 atgctgttaa accccacgca attctcccag caaggattga tgccccagtt tgcaggacag
      601 caggccgggc cgccgcaaat gggtgcgggc ctctttgggc aacccggcgc gtacggcgct
      661 aacggatatc cccccggcgc tcagccgtat ccctttgcgg gacaggggaa tccattcctg
      721 cagagctcgc tcactcagag tccgttcgcg cagaatcctt acctcagcgg cttcggcggc
      781 gcacagcatc cggcccagca gatcgtccag atactcggac agctcgtgca gcacatcttt
      841 acccagagtg cgctgacgca gcagatcggc atcgccatcc agcagctcgc ccagatcacc
      901 gtgcagagcc tgcagtcgca gcaaggccaa ctcggtggcc agctcggtgg tcagctcggt
      961 ggccagctcg gcggacagtc tttcggcctt ggcggcggga tcggcagcca gggcttcggt
     1021 ggcggtggtc aacccttcgg acagagtccg ttcggcgcac agggcggatt cggccagcaa
     1081 ccgcagccgt ggggcctgaa ccgtccgccg acgattcagt agcggattcg accagcacaa
     1141 gacagaacca tggcacgcgg cgagtgcggg aaagccgccc gccgctgccg ctcgcgacgg
     1201 ctccaggggc agcgacgtca tgcaaccgga agaggagggt tcgatggctc cagaaaaaga
     1261 ggagcggccc actgcggcgg ccgccaggaa agccggcgaa gccggtgagg attcggcacg
     1321 gaagacggca ggctcgcaga gccggactcc gcgcgaatcg ggaaacggcg ggaacggcgg
     1381 actgaccgcg gcactccccc ccacgaggcg cgaacgttac atcatcggca cgcgaacaac
     1441 gggtgtgcag ccttttggcc aaccccaaca gcagcactcc atgaacgacg tcgtcgagta
     1501 cctgaagcat caggaaaacg tcgaggtcgt cgaaagaata aaactgggcg gtacggagcc
     1561 cttcactgcc gacgggcgca acgtgagcga ggccgtggtg gccaagattg acccgggaaa
     1621 ggcccagcgg ctccagacca tcgccccacc cgatctggtc atcgaacgcg actcgtcgct
     1681 ggcgtgtgcc gattacctgg ccatgcctgc ccgggtcgcg ccgatcggga cgttgctgcc
     1741 cctgcgctcc gtcgctaccg aggtggtcat tcgtgtgatc ggcgagcgcg atcagccgct
     1801 tgcccgggct accgtcgtca tcgatggagg cggcctgccc gcccaggcgc ttacggatga
     1861 caacgggacc gcacgcgtga cgtttttcgg aggctcgatc gaaaccattc agaccttgtt
     1921 tgtcagatcg gccgcaaacc actgggatcg cgtgatccgc gcgccgcggc tcaacccggg
     1981 ctcgaacaca gtgaggctac gcccgctgtc cgacttctat cccaacttcc ccagtgagcg
     2041 ggtgctcagc tggggccagc gcctgttggg cctcgatccg gcctctggcc gcttctccgg
     2101 cagcggtgtc aggatcggca tcatcgattc gggctgcgac aactcccatc cgctgctacg
     2161 ccacgtcaa
//
LOCUS       DASRHN010000330         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2421015, whole genome shotgun sequence.
ACCESSION   DASRHN010000330 DASRHN010000000
VERSION     DASRHN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2421015"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..1011)
                     /locus_tag="VFB37_13145"
     CDS             complement(304..1011)
                     /locus_tag="VFB37_13145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZO23442.1"
                     /translation="MRYTVLCCDYDGTLASEGRVLPPTVAALERLLASGRRTVLATGR
                     ELDELQSVCPCLGLFEYVVAENGALLYEPCSGRETLLGPRPPEPFVAALRAQGVGPIS
                     VGRVIVATWEPHETAVLETIREQGLELQVIFNKGAVMVLPSGINKASGLTQALNRMGV
                     SPRNAVAVGDAENDHAMLALCGFGVAVANALPALKEKADMVTQGERGAGVAELIDALL
                     RDAVGSRSRTARPAAQG"
     gene            complement(1282..1476)
                     /locus_tag="VFB37_13150"
     CDS             complement(1282..1476)
                     /locus_tag="VFB37_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23443.1"
                     /translation="MTLISFAAPVAHLFNTIQPDDSGVCLLALLAGVIGGWRLTGWHD
                     QLKARRAKIQRDTPRTRKRD"
     gene            complement(1693..2139)
                     /locus_tag="VFB37_13155"
     CDS             complement(1693..2139)
                     /locus_tag="VFB37_13155"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HZO23444.1"
                     /translation="MLQEQELKTFVEGTTNYFEVAAQQPASIGSPYLTEGSPAVHDYT
                     GIISISGKREGIVYFTAPRAMLTVLLMKMQENDFSHETMRDLVGEVANTISGNARRDF
                     GRDFIISVPSVLAGEKPEIPLPPGRRSFVIPINWRSHSAKLVVSLR"
     gene            complement(2146..2547)
                     /locus_tag="VFB37_13160"
     CDS             complement(2146..2547)
                     /locus_tag="VFB37_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO23445.1"
                     /translation="MSVNASYLSGARRSFKLMIVDDSNIMRRRIERSQQFDELELVGT
                     AGNGVDAIELFRRTDPDVVTMDLTMPQMDGIECIAKLVELKPAVRILVISALADKATA
                     VEAMEKGANGFLNKPFTDRQLNEAIAELIRE"
     gene            complement(2544..>2967)
                     /locus_tag="VFB37_13165"
     CDS             complement(2544..>2967)
                     /locus_tag="VFB37_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO23446.1"
                     /translation="AAVHGIESSEVRRAQTKDDVGAVKVEFRRAGEGYELVFEDDGAG
                     ISPEQLKAAAVRKQIMTADEAASMDTRGAMALIFRPGFTTREDVSMDAGRGVGMDVVA
                     RSVYALGGKIGVSTNPGKFTRFKIVLPAVAPASKAMAV"
BASE COUNT          503 a          960 c          962 g          542 t
ORIGIN      
        1 cgggccgcaa gccactgccg ggtcgtcgtg gcataacgtg ccgcgagctc gcggcgcatg
       61 gtgatgatcg ccagagggct agagtgcatc agttcccaat cctgttgtga cagtcctgac
      121 ggggccggag tgcgcgcaga atgacggcag gtcatgacag gtgtacgaca gaaaactcat
      181 ttcatctccc gtaggcgcgg tgtatcacac gtgagcccga gctcgtgtga acggtcatca
      241 cattcgtgaa ttcggcaggg tcaccttcgg tttcgggact gtagggaagc accgcttttc
      301 accttaaccc tgtgcggcgg gccgcgcggt gcgcgacctc gagccgacag catcccgcag
      361 cagcgcatcg atcagctcgg cgacaccggc gccgcgctcg ccctgggtca ccatgtccgc
      421 cttctccttc aatgccggca gcgcgttcgc gaccgccact ccgaagccgc acagcgcgag
      481 catcgcgtga tcgttctcgg catcgccgac ggcgaccgcg ttgcgaggcg agacgcccat
      541 tctgttcagt gcctgcgtca gcccgctcgc cttgttgatg ccggaaggca ggaccatgac
      601 tgcccctttg ttgaagatca cctgcagctc gagaccctgc tcgcggatcg tctcgaggac
      661 ggccgtctcg tgtggctccc aggtcgcgac gatcacgcgt ccgaccgaga ttggccccac
      721 gccttgagcg cgcagtgcgg ccacgaatgg ctccggaggg cgggggccga gcaaggtttc
      781 gcgcccggag cagggctcgt agagcagggc tccgttctcg gctacgacat attcgaacag
      841 gccgagacag gggcagacgc tctgcagctc atcgagctcg cggcccgtgg cgaggaccgt
      901 ccgccgcccg gaggcgagca gccgctctag cgccgcgaca gtgggaggta gtacccgacc
      961 ctcgctggcg agcgttccgt cgtagtcaca gcagaggacc gtgtagcgca taccggggac
     1021 gggaggcgaa gcaagtagca taccggtgcg cggcttgggg cgtgatgtga gcgtggggtt
     1081 gcccccggag gagaacttcg gcgcaagggg gctgccccca cgcccacatc acgcccctgg
     1141 cgtgtgtgcg cgctggagtc ggccgggaag agggctcaga cgcactggcc ccacctgcat
     1201 gggtggcccc aagtgcgcgc tggatgggct gagccgccgc gtccgcgtag cgcggtcagg
     1261 cggggtcgct tcgggtggcg gtcagtcgcg cttgcgagtg cgcggggtat cccgctggat
     1321 cttggcgcgt cgcgccttga gctgatcgtg ccagccggtg aggcgccagc cgccgattac
     1381 gccggcaagc agggcgagca ggcatacgcc gctgtcatcc ggctggatcg tgttgaacaa
     1441 atgcgcgacc ggtgccgcga atgaaatcag agtcattgta gaaaagcccc cgtctggctg
     1501 ggatgaaaca taacagcccg gcggcctgcc gcgggagtcg tccggcggcg acgacgctct
     1561 ttttttgaaa agctcgaaat gcgtcgcgga attcggccgc tcaagacggc gcgcgcagtc
     1621 gctccaagcg gtcccccgcg caaggcggcg gcctctcgac ggggtagatc ggttggatgg
     1681 cgcgcttgcg gcttatcgca gcgagacgac cagcttcgcg gaatggctgc gccagttgat
     1741 gggaatcacg aacgagcggc ggccgggggg cagggggatc tcgggcttct cgccggcaag
     1801 cacgctcggt accgagatga tgaagtcacg tccgaagtcg cgacgggcgt tgcccgatat
     1861 cgtgttcgcc acctcaccca cgaggtcgcg catggtctcg tggctgaagt cattctcctg
     1921 catcttcatc agcagcacgg tgagcatcgc gcgcggcgcc gtgaaataca cgattccctc
     1981 gcgtttgccc gagatgctga tgatgccggt gtagtcgtgg actgcgggag agccttcggt
     2041 cagatacggg gagccgatcg aggcgggctg ctgggcagcc acttcgaagt agttcgtcgt
     2101 cccctcgacg aacgtcttca gctcctgttc ctgcaacatt cgtggctact ctcggatgag
     2161 ctccgcaatc gcctcgttca attgacgatc tgtgaacggc ttgttgagaa agccattcgc
     2221 ccccttttcc atcgcctcga ccgcggtggc cttgtcggcc agcgccgaaa tcacgagtat
     2281 tctcacggcc ggcttgagct ccacgagctt cgcgatgcac tcgatgccgt ccatctgcgg
     2341 catcgtcagg tccatcgtga ccacatccgg atccgtcctg cgaaacagct cgatcgcatc
     2401 gaccccattg cctgccgtac cgaccagctc aagctcatcg aactgctgtg agcgctcgat
     2461 ccgcctgcgc atgatattcg aatcatcgac gatcatcagc ttgaaagatc gtctcgcgcc
     2521 gctgagataa ctggcgttca cgctcatacg gccatggctt tgctggccgg agccaccgcc
     2581 ggcagaacga tcttgaagcg agtgaactta cccgggttgg tgctgacccc gatcttgcct
     2641 cccagcgcat agacactgcg ggcgactaca tccatgccca ccccgcgtcc ggcgtccatc
     2701 gagacatcct cccgggttgt gaatccgggg cggaagatca gggccattgc gcctcgcgta
     2761 tccatgctgg cggcctcgtc ggcagtcatg atctgcttgc gcacggcagc cgccttcagc
     2821 tgctcggggg agatcccagc cccatcgtcc tcgaacacga gctcgtagcc ctcgccggcc
     2881 cggcggaatt cgactttcac cgcgccaacg tcatcctttg tctgggcgcg ccggacttcg
     2941 ctcgactcga tgccgtgcac ggcggcg
//
LOCUS       DASRHN010000331        21433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_785373, whole genome shotgun sequence.
ACCESSION   DASRHN010000331 DASRHN010000000
VERSION     DASRHN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21433
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785373"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..512)
                     /locus_tag="VFB37_13170"
     CDS             complement(267..512)
                     /locus_tag="VFB37_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23447.1"
                     /translation="MIPPSFNGNVEETVMAKRPANNGASWSASELRTLRQLARSGTAQ
                     QAAKHLGRTPAAVQQKAMRTGISFRAGSRGRGRARKK"
     gene            1275..1907
                     /locus_tag="VFB37_13175"
     CDS             1275..1907
                     /locus_tag="VFB37_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016657123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZO23448.1"
                     /translation="MISALEALERLREGNRRFVDVERSSEALVSPRRRLELASGQDPF
                     ATILGCSDSRVPVEIVFDQGLGDLFVIRVGGNIVAPSQIGSVEFAAERFNTRLVVVLG
                     HSRCGVIQATLEQLQDPKVGGSRHVDSIVNRVRPPLEGLMETSLRYDPDALVHHAVRA
                     NVRASVSHLRHGSRIIEQLIQTDGLLVVGAEYSLETGVVDFFDGVPEPTS"
     gene            complement(2180..2725)
                     /locus_tag="VFB37_13180"
     CDS             complement(2180..2725)
                     /locus_tag="VFB37_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23449.1"
                     /translation="MCARVKACNRKWLRRYVQALRENAAVESYITGLSADAVLVPIPP
                     SDSATPAMLWSSHVLACALHHTGLGSSIWVGLRRHRAVTRSSKAWRWERPSVSEHFGS
                     LQVDQPSVSLAQLVLIDDVITKGRTLMAAALRLHHAFPEARIAALALIRTVGFVEEIH
                     RLVDPCEGEIRWNGRDAYRDP"
     gene            complement(2835..3650)
                     /locus_tag="VFB37_13185"
     CDS             complement(2835..3650)
                     /locus_tag="VFB37_13185"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HZO23450.1"
                     /translation="MANFRLQITSVGRGARRRAIAAAAYPAAHAEILLPSRLQQSPAA
                     DWARDRSMLWRAAEQAESRRGGRLAREFQMSLPAELPAQHRLELARAFSRQIAERYAV
                     AVDLAVHDPPASSEPRRFHAHLLATARQVRSAGLGAKTALGMHSRERLHLGLPSESHE
                     FTEMRAQWAALTNEALREAGFDMRVQGRTESAEASIAERPSLHIRRAGVRRLEEIRRE
                     ASRTWLQLRSAGAEQTGALAAAVHDSAAQGAVAPDTDAQTRTRNRGRDDDYLP"
     gene            complement(3685..4896)
                     /locus_tag="VFB37_13190"
     CDS             complement(3685..4896)
                     /locus_tag="VFB37_13190"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HZO23451.1"
                     /translation="MRAWPWSRSLRLAGVAVTVAEETKHFKLIGTTGSGKSTAIAQLL
                     QGALQRGDRAVIADPDGGYLARFHDSGRGDIILNPFELRSMKWDPFSEVQDSYDLEQL
                     ATGLIPASGDGSNQEWRGYARTFLTAVARCCHHRGRRDTAELWRLLAIATSRELRAVV
                     AGTPAQPFLEQDNARMFGSIRSITGSVAAALAHVQAQRGRAFSVRSWIRSGRGVLFMP
                     YRANQIAALRSLIATWMRLAVFEAMNRSEEDHRLWFVMDELDALGAIDGLKDALARLR
                     KFGGRCLLGFQSVAQVTSTYGSGDAQTLLENCGNTLILRCSGSDSGGTARFASRLIGE
                     REMIRRQTSHTQDRLTLLTSYGARRSRNTSEQHVTEPAVMPSEIEQLPDLTGYLKTAS
                     SRAWRHVRIRR"
     gene            complement(4965..5504)
                     /locus_tag="VFB37_13195"
     CDS             complement(4965..5504)
                     /locus_tag="VFB37_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23452.1"
                     /translation="MAFPLQGPEHDPRTPMDEETMKLGLLLEAAQAHQKLAESSLRKL
                     RAQSQDLTSIVRDELRDTLRQELQSLADDGKRAAEALRSLQRVANIRVAAWSLGLTLL
                     CSAVPLALAIWLLPSEGQIRTLSTRHAELAQHIALLEARGGRVDLRRCGVGGRLCVRV
                     DREAPAYGEKSDFLIVKGY"
     gene            complement(5508..6623)
                     /gene="virB11"
                     /locus_tag="VFB37_13200"
     CDS             complement(5508..6623)
                     /gene="virB11"
                     /locus_tag="VFB37_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HZO23453.1"
                     /translation="MISAPQSRPIEAPAPGARQDIAIPARPAALQRPPSEISALDLTL
                     RSLEPLLADPQVTEICLNRPGEAFVETRAGWHQESLPFADFDWCRRLAKLVANSTRQR
                     VDESSPLLSASLPGGERIQIVLPPATTAGCVAITIRRPSEEVWSITELAGRGIFRQTR
                     RATEALDATDTRLLRLLDIGDYEAFLRLAVVSRKNILVSGATGSGKTTWTKALIREIP
                     EDERLITIEDARELVLDHHPNHVRLYYSKDDQGLARVTPKQLLEACLRMKPDRILLAE
                     LRAEEAFDYLRNVNSGHPGSITSVHAASAELAFEQLVLLVKQSPGGRELARGDIQTLL
                     YLLVDVVIQCAIEHHERFIREIWFDPERRRRCLSERR"
     gene            complement(6592..7791)
                     /gene="virB10"
                     /locus_tag="VFB37_13205"
     CDS             complement(6592..7791)
                     /gene="virB10"
                     /locus_tag="VFB37_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HZO23454.1"
                     /translation="MNEVPAQDVQGERGTPSVSRVRSLQSRLSSIVACTLMLIVGAAM
                     LTWYYATALSRQRHARRAEQATLAMRAQSEMPLPSLGKIDSWPAGAPRGPGSAVPSAP
                     AGPAPDSPPQPLPSLPLAPAPQSSAISMPLAYGAAPTPYGTPSLKSSSQSALERRLSG
                     VAFASTASPSAAPTEAAGSGNGPHSESELASLLRPDVAPPAQARYLSAPRFLLAKGTF
                     IDCTLETAIDSTLPGMTACVTATDTFGADGKVVLLERGTKLIGETRGQVQQGAARVFV
                     LWTEARTPAGIIVPLDSPGADELGRSGLSGEVNHHFWDRFGAAILISTLDGAVQAAVQ
                     STSRNSGTVIYNPATSEGIVTDVLKGTLNVAPTVTKENGERIQVFVARDLDFRPVYDL
                     RTAVAAH"
     gene            complement(7788..8633)
                     /locus_tag="VFB37_13210"
     CDS             complement(7788..8633)
                     /locus_tag="VFB37_13210"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HZO23455.1"
                     /translation="MSKTWGRVGSADAHALSACMAALLMFGASHGRTETLPARGKVDS
                     RVREVSYNREEVYRLHGLVGYQIDLQFESGESFVGLGSGDLDALSFVAAGNHLFIKPK
                     AAQVSTNLTVLTTKRAYQFDYTASVRRADTAGEGVIYSLRFTYPPSHADARAADHLEK
                     LLDAPDSSKTINSDYAYCGSPSIRPIAASDDGVHTRLRFGSRSEQPAIFVHNDDGSES
                     LLNFSMESGDVIVHRVVRELILRRGRLTGRVVNGGFVGSGQRLESGTVSPAVERMDAR
                     DTQQP"
     gene            complement(8626..9363)
                     /locus_tag="VFB37_13215"
     CDS             complement(8626..9363)
                     /locus_tag="VFB37_13215"
                     /inference="COORDINATES: protein motif:HMM:NF016238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZO23456.1"
                     /translation="MSTDAELDQYLQEARSWDADRLSQARRSSRMAWWAAAAGWSCAL
                     AGAASLVLLLPLKSVEPFLVRVDNSTGIVDIVPVYTGHAAMDEAVTRYFLVHYITVCE
                     RFNFATAESDYEECGAFQTAQRNQAWYAMWNKSNPASPLNMHKDGSVVRAQVESVSFI
                     KRASGVSDLAQVRYAKSEQQSGGSAEKLTHWIATIQYSYGSPSKNARSRGWNPLGFKI
                     VELTSEPEVLTSSISSDRPQSKGMRDE"
     gene            complement(9353..9520)
                     /locus_tag="VFB37_13220"
     CDS             complement(9353..9520)
                     /locus_tag="VFB37_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23457.1"
                     /translation="MKKLSTSIVPLAALMLLAACASHRPRCESLQPINAVALPAAQVP
                     ERAPDETAHEH"
     gene            complement(9561..10472)
                     /locus_tag="VFB37_13225"
     CDS             complement(9561..10472)
                     /locus_tag="VFB37_13225"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZO23458.1"
                     /translation="MGFFQSFWSWLNGQLATYIGDNTMRVAEALEPAIVTLATLYVMV
                     WGYLQLTGRIEEPFVSGLKRIVTLAVILAVSLRLWLYNTVIVDSFYTAPTQLASLVVG
                     SSDPVQTIDLVWERGGAVAGFLWNKGGVLNGDVGYYVAGVLVWLLIGLLCVYTMFLIA
                     LSHVALAILLALGPLFITMMLFDSTRRLFDAWIAQLANYALVTILTVMAGALFLQIVE
                     SYAAQTAARGSSIRTMDTLDMMLVSVLVFLFMRQVMPIASGLVSGAALSTLGTMSRAL
                     QWSMRSGVASLRFGARAGAALLMRERP"
     gene            complement(10510..11247)
                     /locus_tag="VFB37_13230"
     CDS             complement(10510..11247)
                     /locus_tag="VFB37_13230"
                     /inference="COORDINATES: protein motif:HMM:NF019608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZO23459.1"
                     /translation="MRAPRKFRRAVSIRFGACVLVSGCLLTGPRAHAQFAVIDVGAIA
                     QLVGQAQTLEQQLVTAQNQLAQATAEYASITGGRGMESLLAGAPRNYLPTNWSDLQAV
                     LNGGGGRYGMLSAGVAGSMQLNAVLSPQQLAALPADASLQAQSARSLTALAQNVAREA
                     LQTTSGRFDSLQQLIGTLPRATDQKAVLDLQARIAAENAMLQNEQTKLQTLDRVLRAQ
                     AQAEQEQIWERTIAGHGQFATRFEPAP"
     gene            complement(11244..13664)
                     /locus_tag="VFB37_13235"
     CDS             complement(11244..13664)
                     /locus_tag="VFB37_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HZO23460.1"
                     /translation="MSAMRREDAAAVRIPCAAHVAPHVLRTVSGDYLQVMKLSGASFE
                     TADDGQVNTWHERLNVLWRNIASPNVALWTHVIRRRERTPEPGEGPGGFAGRLAAKYA
                     RRLAAETLMINEVFLAALYRPAAGVTTGILSRALARAQRDAPRLELADALDACEKLGQ
                     TLRASLARYDPETLGTYRLGEVWYSSILEFLGLLVNGEWQRMPLPRCAVSEAIATTRL
                     FFGAETIEYRTPTRTRLGAMLGIKEYPTPSVVGMYDRLLSAPFPMVLTQSFAFLAKPT
                     SQALLQRQFYRMQNAGDFAVSQAEELKDALDALTSNEFVMGDHHFSLQVQCDPGEGEA
                     ARRHGTLNNYVALARSLLADTGMTVAREDLALEAAFWAQLPGNFAMRPRKAPISSRNF
                     AAMAAFHNYPAGRATGNHWGAALALLMTSARSPYYFSLHASHSTDGEPSGPKDTGHTF
                     ICGPTGSGKTVFIGFLVAMLEQQGATQVIFDKDRGLEILVRALGGEYLPLRNGTPTGF
                     NPLQLPALPAHIEFLKSWLRALVRFGGSLAPSTREQADLDQALRGTLALERKARRLSR
                     LIEFLDPTDPEGIHARLACWCEVSRGDYAWVFDNDEDTVVARMEARALVGFDVTEFLE
                     HELTRTPVTLYLFHLVRQLLDGRRLVCWLDEFWRMLSDPAFESFAKDAPKTWRKLNGV
                     MCFATQSASDVLESAISRTIIEQTPTKVFFPNLNASPAEYSGFGLTQREFKLIKEQLE
                     PGSRRFLIKQGPHSVVCQLDLRGFDAELAVISGRASQLARMQRMMSAQGKDPAAWLTA
                     FMNTESQE"
     gene            complement(13676..14071)
                     /locus_tag="VFB37_13240"
     CDS             complement(13676..14071)
                     /locus_tag="VFB37_13240"
                     /inference="COORDINATES: protein motif:HMM:NF016957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HZO23461.1"
                     /translation="MSGSSSALIADPLFVGATRPPMRWGVTYSALLFNLVFTMELFLL
                     TRNLLALLASIPIHAICALLCARDARIFDLAMLWLRTRLPALLGSLRYWKASSYSPLE
                     LDIPSVRGARRRRDLDLATLRVCYQVRAC"
     gene            complement(14079..14378)
                     /locus_tag="VFB37_13245"
     CDS             complement(14079..14378)
                     /locus_tag="VFB37_13245"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HZO23462.1"
                     /translation="MKLRPTNVRGCSLALAVALAGAPALAFGQASPFLTGATSLQNNI
                     LAWLTPIAIILIMVLGAMAMANRMSWGWCIGAILGIAIAFGAPQIVSWVRGMFGV"
     gene            complement(14465..14866)
                     /locus_tag="VFB37_13250"
     CDS             complement(14465..14866)
                     /locus_tag="VFB37_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZO23463.1"
                     /translation="MNSFTLLAIGNLARNPELVAKGDTMYTRFCLVGNDYAGKDEEGA
                     AREVITSLWFVAFGALGESLAKNARKGDQLVLEARIRSHNWTDKQGEKQYDHSFIVQG
                     FRFGAPGRAKRQEFDAQQDEEDGRVVLEAEA"
     gene            14971..16482
                     /locus_tag="VFB37_13255"
     CDS             14971..16482
                     /locus_tag="VFB37_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZO23464.1"
                     /translation="MFAQKFAPRRVYPSLTIRNENLPAKLMMADARSVIPSLLARLTG
                     PVLASLFLAACANVPVVDETLSSTYSPSEIKIMGARGPLSARQSQAVLKHLAAQAPDA
                     GALERHLAIEQAVAESPLFTGNRVQILRDGSQTFPAMFEAIHSAQHYLLLEYYIFEDV
                     TCNGEHLGDLLVEKAKAGVKVNVIYDGIGSIGTPSSFFDRLKEAGVQLLEFNPPNPLR
                     SVRHFSVNDRDHRKMLIADGNVVMVGGVNLSSTYESGPSGSDAGSGEKQKKADKAAPE
                     VWHDTDLEIWGPVVIELEKLFREHWNEQKGTPLDINLQPPGERMGNEVVRIIGSAPRR
                     LRTRYYVTVLTAIRSAENSIWITSAYFVPTHQEKRDLIRAARKGVDVRLLLPSRSDSS
                     AALAVQHSEYSDLLRAGVKIFERDDGILHSKTMVIDGVWSITGSSNFDHRSILFNDEV
                     DAVVIGKNTGEELAKLFQSDLEHAKPIDLASWRKRSTLTKLREQFWRLWENLL"
     gene            complement(16609..18726)
                     /locus_tag="VFB37_13260"
     CDS             complement(16609..18726)
                     /locus_tag="VFB37_13260"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HZO23465.1"
                     /translation="MGYRRLFSRHPIAIAAFLVLAAAAMAVAGADEGDPPGRVARLSD
                     AEGSVSLQPAGVQDWAAAAPNRPLTTGDKLWTDQNSRAELDLGSAAIRLGSTTGFSFL
                     NLDDRMAQMNVTAGTAIVHVRDMGENQAFEIDTPNIALTLQAPGDYRVEVNEAGDTTL
                     VAVSNGQAEASATGQTIPIGTQQAVTFTGTDQVSADASSVGAPDALDQWSMDREHQEE
                     QAQAQASQYVPPDMAGAEQLNQYGSWSSVPEYGSVWTPNDVGPGWAPYQDGRWVWISP
                     WGWTWVDNAPWGFAPFHYGRWANAGGRWFWVPGPRHVRHVYAPALVAWVGTPGGVATT
                     VAVGAGVAAVAWFALGPREVYVPGYHVSPAYVRNVNVSNTTIVNNTYITNVYENKVTN
                     ITYVNQHAPGAITAVSRSTFVSAQPVRGHTVAIAPAELGRMRPVGAAPAIAPERQSVL
                     GSVAGRDVHRPPAAFSNRPVVARVAPPPAPVSFAKQQEAIRANGGRPLAGPQLATIQE
                     RVPTSRVRVVTPGPVHSLPAVRSAPAPSLQQRERALQATPVPPPQNAVRGDRPPGAQR
                     SAAPPESSSNRESGRPIPQAHPLPQAHPLPRARPLPEARPLPEAHPLPEAHPLPEAHP
                     PQDRTIEPEAPRAVTPPSAAPAPRIESPRPGSPQAHPAAPPGAARPAPERRPPPPPAR
                     AEERRSNPPDRPNNRQDRPNNRP"
     gene            complement(18839..19330)
                     /locus_tag="VFB37_13265"
     CDS             complement(18839..19330)
                     /locus_tag="VFB37_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23466.1"
                     /translation="MAANLPTPMRAGLRMAIYIVFGALWVSGCLWLLLHEFFTHPGDF
                     GPLPNPWEPAVLRLHGWIAVTAVFLLGWITARHVSDRWGQTIKRPSGLAVVGVAVVLV
                     LSGYALYYTTDGLHDIAAAAHEMLGAAGLLFALLHWRRYRPRTAARKLAQPPPPESSF
                     PAT"
     gene            complement(19336..19908)
                     /locus_tag="VFB37_13270"
     CDS             complement(19336..19908)
                     /locus_tag="VFB37_13270"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZO23467.1"
                     /translation="MCEHSLALLGGAALVSFGAAPTQVVEAADYLSVADAQHELFSQA
                     DRFEEIVLALTPAQKQQVAQLAGPQPPHRSLRAWRAFHGGDLLGHVFVDEVLGRQDMI
                     TYAVAIDRAGRMSALEVLSYRESHGGEIRGKAWREQFDGRQDLPALRLGTEIKNIAGA
                     TLSCEHVTQGARWVLALWQVALRTAGAPES"
     gene            20097..20912
                     /locus_tag="VFB37_13275"
     CDS             20097..20912
                     /locus_tag="VFB37_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZO23468.1"
                     /translation="MNYMQAPADPVPPHPETAKVRRMRALLGTFVVIEATAPCVEAAH
                     SAIRAAFATIAELQTLLHPGTARGDLTRIREASPGTPVRVAASTWDLLRLASELSALS
                     EGVFDPSLPHRPGKLRDLELLAEHRILAHVPVALDFGGFAKGHAVDRSIQVLVSHGCR
                     SGLVNAGGDMRAFGADPWPVALRGSDGNFKTFELADAALAVSDSSSQEAPSEHQGYYN
                     RAWPQDGLICTRAAVVAREAVLADALTKCLMLCRQAVAARVLQAFEAFALPLT"
     gene            complement(20905..>21433)
                     /locus_tag="VFB37_13280"
     CDS             complement(20905..>21433)
                     /locus_tag="VFB37_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO23469.1"
                     /translation="VVEAARKAHAHDFILQIRDQYASLVGERGVKLSGGQRQRIAIAR
                     VVVKNAPILILDEATSSLDSITEQAIQETLDDAMRGKTVIVVAHRLSTIAHLDRILVF
                     DEGRIVEDGTHTQLLARGGAYYRLWSRQAGGFVLDDGAAASPDSVGMEEELPDAGEPP
                     EEVLGHARSPTERTM"
BASE COUNT         3835 a         6947 c         6933 g         3718 t
ORIGIN      
        1 tgagcccatc aacctgcgtc tgttcctgtc cgtggacggc caggcgctct cggaaacctg
       61 gctatatcag tacgtgccgc cgccgccgga tcaacgccac ctgtaggcca accgcactgc
      121 aacagactaa cgccggcggc gttatgtcac aaagaggtgg aaaagcccgg gctgccctgc
      181 ctctccgcca cgcgtgctcc gatcaaccgc gccttcgcgg ccggaccggc gcggccggca
      241 tccccgggcc cctccgctag ccccgcttat ttcttccggg cacgtccccg tccgcgcgag
      301 ccggcgcgga acgaaatacc cgtacgcatc gccttctgct gaaccgccgc tggcgtgcgt
      361 cccaagtgct tggcagcctg ttgggcggtg cccgagcggg cgagctgccg cagcgtgcgc
      421 agttccgatg cgctccacga tgctccgttg ttggctggac gtttggccat caccgtctcc
      481 tcgacattac cgttgaacga gggcgggatc atacacggct tcgcggcgga actgacagtg
      541 atgtttagtg cgcgagggga cacgggaggc caacctgact tcccctgaaa aagtcaagac
      601 cttgacgata cgctgttgag ttggaggtcg gtccagatcg atgcggcgcg acggcgccag
      661 cgcagggttg actccgctgc cgtataactt gcgcagggcg gcaaaatggc ggtcgtgcgt
      721 cggacggtcg agcagtgacc acgggccggc cgcctgcggg gtccttgcgt tccgggaatg
      781 cctccgtgag atcccgcagc tccgaatcga gggtatccac cgtcaaggtc acgaccgcaa
      841 catcgtgttg cgttaaggcg ccatcgagca cgaaaccaaa ctcggtcagc tccttgtgtg
      901 ccgcgacgtc atcgagcacc acagcctgct caccatcccg atcgcctgaa gacaggccac
      961 gagatcctgc cggtggagcc gagaggcggc gatcgaacac ccggtcggaa tgcgccacca
     1021 cctgggcgca attggcaaac ccccagcgtg aaagtacggt gcattgagtt ggcgttcagc
     1081 agcgtcgggg gtctcgaacg gcccttggac ggggccagta acaatccgtt cgggaccccg
     1141 agcttcggcg gagcggccaa cgacgcgcag gccgcggact ccaccggcgc cggaggcgcg
     1201 cggcagaaag caccgcccgg ccttttccat gggctaaaat acggctccct tcgagcgccg
     1261 ggagcctttc gccgatgatt tccgcattgg aggccctgga gcgccttcgg gagggcaatc
     1321 gccgctttgt ggatgtcgaa cgcagctccg aggcgctggt cagtccgcgg cggcgcctcg
     1381 agctggcctc gggtcaggat ccgttcgcca ccatcctcgg atgctccgat tcgcgggtgc
     1441 cggtggaaat cgtgttcgat cagggactcg gagacctgtt cgtcatccgt gtgggcggga
     1501 acatcgtggc gccttcacag atcggcagcg tcgagtttgc cgccgagcgc ttcaacaccc
     1561 gcctggtggt cgttctgggc cactcgaggt gcggagtcat tcaggccacg ctcgagcagc
     1621 ttcaggaccc aaaagtcggc ggctcacgcc acgtcgattc gatcgtgaac cgggtccggc
     1681 cgccgctgga gggcctgatg gagacgagtc ttcggtacga tccggatgcc ctggtgcatc
     1741 atgctgtgcg ggcgaatgtg cgcgcttcgg tgtcacattt gcgccatgga tctcggatca
     1801 tcgagcagct catccagacg gacggactgc tcgtggtggg cgccgagtac tcgctcgaga
     1861 cgggcgtcgt ggacttcttc gacggcgtgc cagagcccac gtcctgagca agaatcggaa
     1921 tgtcgcagcg gcgcttgccg ggcacactgt ctgtaacagg gcacggcccc tccaaggagc
     1981 gccgcatgag ctttcggatt tcggggctgc cagtctgcag gatgccgcac cgggcgaaac
     2041 attgttgttg ttgaattacg aacacctgtc ggtggcttcg ccctatcgct caaggtacgc
     2101 tatctacgtg cgtgagggcg tgcatgtcca caatgccaaa cccgggtgct atgccgcgcg
     2161 cgtagatcgg gcctgaaacc tacgggtcgc ggtacgcatc gcggccattc cagcggatct
     2221 ccccctcgca ggggtccacg agccgatgga tctcctccac aaatcccacc gtgcggatca
     2281 acgccagggc ggctatgcgc gcctctggaa aggcatggtg cagtcgcagt gccgccgcca
     2341 tcagcgtgcg tcccttcgtg atcacatcat cgatcaggac cagctgtgca agactcacgc
     2401 tcggctgatc gacctgcaac gacccgaaat gctcggacac ggaggggcgc tcccatctcc
     2461 aggctttcga tgagcgcgtg acggcgcgat ggcggcgcag accgacccag atggagctgc
     2521 cgagcccggt atggtggagc gcgcacgcga gaacgtgcga ggaccacagc attgcgggtg
     2581 tcgcactgtc gcttggggga atcggaacga ggacggcgtc cgccgacagc ccggtaatgt
     2641 acgattcgac tgctgcattt tcgcgcaggg cctgcacgta acgccgcagc cacttgcgat
     2701 tgcacgcctt cacacgcgcg cacatgaggc gcgacctctt ggacaagacg ctttcacctt
     2761 gtggtgagta aacgtagcag gcccaaaacg gaatccgata cttacgcggc aaccgttcga
     2821 tctgcggacc gcggtcaggg aagatagtcg tcatcgcggc cacgattgcg cgtgcgagtc
     2881 tgcgcatcgg tgtcaggcgc gaccgcgcct tgcgccgcgg aatcatgtac tgccgcggcg
     2941 agtgctccgg tctgctcggc accggcagat cgcagctgca gccaggttcg gctggcctcg
     3001 cgtctgattt cctccagccg tcggacgcca gctcggcgga tgtggagtga tggcctttcc
     3061 gcaatcgagg cttcagccga ttcggtgcgt ccctgaacgc gcatgtcgaa acctgcctcg
     3121 cgcagggctt cgttcgtcag agcggcccat tgggcacgca tctcggtgaa ttcatgcgac
     3181 tcgctcggca ggcccagatg gagtcgctcg cgcgaatgca tgccgagggc tgtcttggct
     3241 ccgagccccg cggatctcac ctgccgggcg gtcgcgagca gatgggcgtg aaaccggcga
     3301 ggctcgctgc tcgcaggcgg gtcatggaca gcgaggtcga ccgccactgc gtatcgctcg
     3361 gcaatctggc gcgaaaaagc acgtgccagc tcgagtctgt gctgtgccgg caactccgcc
     3421 ggcaggctca tctgaaactc gcgggcgagc cggccgcctc ttcggctctc cgcctgttca
     3481 gcggcgcgcc acaacatcga gcgatcgcgt gcccaatcgg cggcggggct ctgctgcagc
     3541 cgcgagggca gcaggatctc cgcatgcgct gccggatagg cggcggccgc aatggcgcgc
     3601 cgtcgcgcac cgcgcccgac cgaagtgatc tgcaaacgaa aattggccat gggcgactcc
     3661 ctgcccgagc ttcggcttcc atgcctatcg gcggatgcga acgtgtctcc aggccctgga
     3721 ggaggctgtc ttgagatagc cagtcaggtc cggaagctgt tcgatttcgg agggcatgac
     3781 ggccggctcc gtgacgtgct gttcgcttgt gttgcgcgag cggcgcgccc cgtagctggt
     3841 caggagggtg aggcgatctt gggtgtgtga ggtttgccgc cggatcattt cgcgctcacc
     3901 gatcagtcgt gaggcgaaac gcgcggtacc gccagaatcg cttcctgaac agcgtagaat
     3961 caatgtgtta ccgcaatttt ctaagagcgt ttgcgcatca ccggagccat acgtactcgt
     4021 gacttgtgcg acggattgaa acccgagcag gcagcggcct ccgaatttgc gcagccgtgc
     4081 caaagcgtcc ttgagcccat cgatcgcgcc gagcgcatcc aactcgtcca tcacgaacca
     4141 caggcgatga tcctcctcgg agcggttcat cgcctcgaac acggcgagcc gcatccaggt
     4201 cgcgatcagc gaacgcaacg cggcaatctg attcgcgcga taaggcatga acagcacgcc
     4261 gcggccgctg cgaatccagc ttcgcaccga gaaggcacgc ccccgctgcg cttgcacgtg
     4321 ggcgagcgca gccgccaccg atcccgtgat cgagcggatc gaaccaaaca tccgtgcgtt
     4381 gtcctgctcc aggaaaggct gagcgggtgt gccggccacg acggcgcgca gctcgcgact
     4441 tgtggcgatg gccagcaggc gccacagctc cgccgtatca cgccgacctc tgtgatggca
     4501 gcagcgtgcg acggcggtga gaaaggtgcg cgcgtagccg cgccactcct gatttgaacc
     4561 gtcgccactt gcaggaatca acccggtggc gagctgctcg agatcataag agtcctgtac
     4621 ctccgagaag gggtcccact tcatggagcg gagctcgaac ggattgagga tgatgtcccc
     4681 acggcccgaa tcatggaacc gggcgagata gccgccgtcc ggatcggcaa tcacggcgcg
     4741 atcgccgcgc tgcagagcac cctgcaacag ctgtgcaatg gctgttgatt tgcccgatcc
     4801 ggtagtgccg atgagcttga agtgcttggt ctcctcggcg acggtgaccg ccacaccggc
     4861 cagccgcagt gagcgactcc agggccaagc gcgcatgcgg agcccgctct gcccgaacga
     4921 tggccctctg acgaggagcg tgccacgttt gcgccattcc atgctcaata ccccttgacg
     4981 ataaggaagt ccgacttttc tccataagca ggcgcctccc gatcaactcg gacgcaaagc
     5041 cggcccccca caccacaccg ccgcaaatca acgcgcccac cccgagcttc gagtagcgca
     5101 atgtgctgcg cgagctccgc atgtctggtg ctgagcgtgc ggatctgccc ctccgacggg
     5161 agaagccaga tcgccaaagc cagcggcacc gccgagcaca acaaggtcag accgaggctc
     5221 caggccgcga cgcgaatgtt cgcgacgcgt tgcagggatc gcagcgcctc cgccgcgcgc
     5281 ttaccatcgt ccgcgagtga ctgcagctcc tgcctcagcg tgtcccgcag ctcatcgcgc
     5341 acgatgctcg tgaggtcctg cgattgagcc cgcagcttgc ggagactgct ctcggcaagc
     5401 ttctgatggg cctgcgccgc ttccagaagc aggccgagct tcatggtctc ttcgtccatg
     5461 ggagttcgtg ggtcgtgctc gggtccctgc aggggaaatg ccatgggtta ccggcgctcg
     5521 ctgagacagc gacgccgacg ctccgggtcg aaccagattt cgcggatgaa gcgctcgtga
     5581 tgctcgattg cgcattgaat gacgacatcc acgagcagat ataacaaggt ctgaatgtcg
     5641 cccctcgcca gctcacggcc cccggggctc tgcttcacga gcagcacgag ctgctcgaaa
     5701 gcgagctcgg cacttgcggc gtgcacgctc gtgatcgagc cggggtgacc ggagttgaca
     5761 ttgcgcagat agtcgaaggc ctcctcggcg cgcagctcgg cgagcagaat tcgatcgggc
     5821 ttcatgcgca ggcacgcctc gaggagctgt ttcggagtga ctctcgcgag gccctggtcg
     5881 tccttgctgt agtacagccg gacatgattc ggatggtgat cgagcaccag ctcccgggca
     5941 tcttcgatgg tgatcaggcg ctcgtcttca ggaatttcgc gaatcagtgc cttcgtccag
     6001 gtggtcttac ccgaaccggt cgcgccggag accaggatat ttttccggct cacgacggca
     6061 aggcgcagga aggcttcgta gtccccgatg tccagcagcc gaagcaggcg cgtgtcggtc
     6121 gcatccagcg cctcagttgc acggcgcgtc tgacggaaga ttccccgccc tgccagctcc
     6181 gtgatcgacc acacctcctc ggacggccgt cggatcgtga tggctacgca tcctgcggtg
     6241 gtcgccggcg gcagtacgat ctggatacgc tcacccccgg ggagcgaggc cgaaagcagc
     6301 ggagaggatt catccacgcg ctgacgcgtg gaattggcga cgagcttcgc gaggcgccgg
     6361 caccagtcga aatccgcgaa cggcagcgat tcctgatgcc agccggcccg agtctcgacg
     6421 aatgcctcgc ccggccggtt gagacagatt tccgtgacct gcggatctgc caggagtggc
     6481 tccagggatc gcagcgtgag atcgagcgcg gagatctcgc tgggtggacg ctggagggcg
     6541 gccggcctcg cagggatcgc aatgtcctgc cgcgcaccgg gcgccggtgc ttcaatgggc
     6601 cgcgactgcg gtgcggagat cataaacggg cctgaagtcg agatcgcgag ccacgaagac
     6661 ctggatgcgc tcgccgttct ccttggtcac cgtgggggcg acattgagcg ttcctttcaa
     6721 cacatccgtc acgattcctt ccgaggtcgc ggggttgtag atcaccgttc cgctgttgcg
     6781 gctggtggat tgcacggccg cctgcacggc gccatcgagc gtggagatga ggatcgctgc
     6841 tccgaatcga tcccagaagt gatgattcac ttcgcccgag agaccggagc gcccgagctc
     6901 atcggcgcct ggagagtcga gcggtacgat gattccggcc ggtgttcgcg cttccgtcca
     6961 cagtacgaac acgcgggcag ccccttgctg aacctgccct cgcgtttccc cgatcaactt
     7021 cgttcctctc tccagcagca cgacttttcc gtccgccccg aacgtatccg tggccgtgac
     7081 gcatgcggtc atgcccggga gtgtggagtc gattgcggtc tccagggtgc agtcgatgaa
     7141 tgttcctttc gcgagcagga accgcggggc cgacaggtag cgcgcctggg ccggcggtgc
     7201 cacgtccggt ctcaacaggg acgccagctc gctctcggaa tgtggaccgt tgccggagcc
     7261 tgcggcctcg gtgggcgcag cactcggtga agcggtactc gcaaatgcga cgcccgacag
     7321 gcgccgctcg agcgcacttt gcgaggagct tttgagcgaa ggtgtgccgt aaggtgtggg
     7381 ggcggcgcca taggcgagag gcatgctgat tgcgctcgac tgcggcgccg gtgccagtgg
     7441 aagtgagggc agcggctgcg gcggcgagtc cggcgccggt cccgctggcg ctgagggaac
     7501 ggctgagccg gggccgcgcg gtgcaccggc cggccagctg tcgattttgc ccagcgacgg
     7561 aaggggcatc tcgctctggg ctcgcatcgc aagtgtcgcc tgttctgccc ggcgcgcatg
     7621 gcgctgtcgc gacagcgctg tcgcgtagta ccacgtcaac atcgcagcgc ccacgatcag
     7681 catcaacgta caggccacaa tgctgctgag gcgggattga agagagcgaa cgcggctcac
     7741 cgagggcgtg cctctttcgc cttgcacgtc ctgtgccggc acctcgttca tggctgctgg
     7801 gtatcccgcg catccatgcg ctcgacggcg ggcgatacgg tgccggattc aaggcgctgg
     7861 ccggagccaa cgaatcctcc attcaccacg cgccccgtca gccggccgcg ccgcagaatc
     7921 agctcgcgca cgacgcggtg cacgatcaca tcgcccgact ccatgctgaa gttcagcagc
     7981 gactcgcttc cgtcgtcgtt gtgcacgaat atcgccggct gctcggagcg gctgccgaag
     8041 cgcaagcgcg tatgaacgcc gtcgtccgaa gcggcgatcg gtcggatcga ggggctgccg
     8101 cagtaggcgt agtctgaatt gatcgtcttg ctggagtcgg gcgcatcgag cagcttctcg
     8161 aggtgatccg ctgcacgagc atctgcgtgt gaaggcggat aggtgaagcg caaggaatag
     8221 atgacccctt cgccagcggt gtctgcgcgg cgcacggagg cggtgtagtc gaactgatag
     8281 gcccgcttcg tggtgagcac ggtcagattg gtgctcacct gcgccgcctt gggcttgatg
     8341 aagaggtggt tgcccgctgc gacgaaggag agcgcatcga gatcgcccga gcccaggccg
     8401 acgaatgact cgccggactc gaactgcagg tcgatctggt agccgacgag gccatgcagc
     8461 cggtagacct cttccctgtt gtaggacacc tcgcgcaccc ggctatccac cttgcctcgg
     8521 gccggcagag tctcggtacg gccgtgcgaa gcaccgaaca tcaagagtgc tgccatgcag
     8581 gcggacaagg cgtgcgcatc cgccgacccc accctccccc atgtcttact catcgcgcat
     8641 ccccttcgac tgcgggcggt ctgaggagat cgaggaggtc agcacctcgg gctccgaggt
     8701 caactccacg atcttgaatc cgaggggatt ccagccgcgg ctgcgcgcat tctttgacgg
     8761 ggatccatat gaatattgga tcgtcgcgat ccagtgagtc agcttctcag cggatccgcc
     8821 gctttgctgc tccgatttcg cataccgcac ctgcgcaaga tcggacacac cgctcgcccg
     8881 tttgatgaag ctcacggatt ccacctgagc gcgaaccacg ctgccatcct tgtgcatgtt
     8941 cagtggtgag gctggattcg acttgttcca catcgcgtac cacgcctgat tgcgctgcgc
     9001 cgtctggaag gctccgcatt cctcgtaatc gctctcggcg gtggcaaagt tgaagcgctc
     9061 gcacaccgtg atgtagtgaa cgagaaagta gcgcgtgacg gcttcatcca tggcggcgtg
     9121 cccggtgtac acgggaacga tatcgacaat gcccgtgctg ttatccacac ggaccaggaa
     9181 gggctctacg cttttcagag gcagcagcag caccagcgac gccgcacctg ctagtgcgca
     9241 cgaccagcca gcagcggcag cccaccaggc catccgtgaa ctgcgccggg cctgactcag
     9301 gcgatccgca tcccatgagc gggcctcctg caggtattgg tccagctcag catcagtgct
     9361 catgcgctgt ctcatccggt gcgcgctctg gaacctgggc ggcggggagc gcaaccgcat
     9421 tgatgggctg caggctttca caccgcggcc tgtgcgaggc acacgccgcg agcaacatca
     9481 gggcggcgag cggcacaatg gatgtcgaca gctttttcat gacctctccc tggcgcctgt
     9541 tccggcacgg cgagcgcgac ttacggcctc tcgcgcatga ggagtgcggc ccccgcgcgg
     9601 gcaccgaatc gaagcgaggc gacacccgat cgcatgctcc attgcaacgc ccggctcatg
     9661 gtgcccagcg tgctgagcgc cgcgccgctg accagtccgg aagcaatggg catgacctga
     9721 cgcatgaaca aaaatacgag caccgacacg agcatcatgt ccagagtgtc catggtccgg
     9781 atcgaggagc cgcgcgctgc agtctgggcc gcgtacgact ccacaatctg cagaaacagt
     9841 gctccggcca tgacggtgag aatcgtcacg agagcgtagt tagcgagttg cgcaatccag
     9901 gcatcgaaaa ggcgcctcgt gctgtcgaac agcatcatgg tgatgaacag cggtccgagc
     9961 gccaggagaa tggccagcgc tacgtgcgac agggcaatca ggaacattgt gtagacacac
    10021 agcagcccga tgaggagcca cacgagcaca cctgcgacgt agtagccaac gtccccgttc
    10081 agcactccgc ccttgttcca caggaagcca gcgactgctc cgccgcgctc ccagaccaga
    10141 tcgatcgtct ggaccgggtc ggaggagccc accaccaggg aggcgagctg ggtgggggcg
    10201 gtatagaagc tatctacgat gactgtgttg tagagccaaa gtcgcagcga aaccgcgagt
    10261 atcacggcca gagtcacgat gcgcttcaag ccggacacga aaggctcttc gatccgccca
    10321 gtgagctgca gataccccca gaccatcacg tagagcgtcg ccagggtcac gatggccggt
    10381 tccagggctt ccgccacgcg cattgtgttg tccccgatat aggtcgcgag ctgtccgttc
    10441 agccacgacc agaagctctg gaaaaatccc actttcgcct cctgcaaccc tggcggacgc
    10501 cactgccgct cagggagccg gctcgaaccg ggtcgcgaac tgtccgtgcc ccgcaatcgt
    10561 tcgttcccag atctgctcct gctcggcctg ggcctgcgcg cgcagtaccc gatcgagcgt
    10621 ctgtagcttg gtttgctcgt tctgcagcat cgcgttctca gctgcaatcc gggcctgaag
    10681 gtccagcact gccttctggt cagtcgcgcg cggcagggtt ccgatgagct gctgcaggga
    10741 atcgaatcgc ccgctcgtgg tctggagggc ttcgcgtgcg acgttctgcg ccaatgcagt
    10801 gaggctgcgt gcagactgcg cctgcaaact cgcatccgcg ggaagtgcgg ccagctgttg
    10861 cggtgagagc acggcgttga gctgcatgct gccagccaca ccggcggaga gcatcccgta
    10921 gcggccaccg ccaccattga gcacagcctg gagatcgctc cagttcgtgg gcagataatt
    10981 gcgtggggcg ccggcgagca ggctctccat cccgcgccca ccggtgatgg aggcatattc
    11041 cgctgtggcc tgtgcaagct gattctgtgc cgtcaccagt tgctgctcga gcgtctgggc
    11101 ctgcccgacg agctgcgcaa tcgcacccac atcgatgact gcaaactgcg cgtgcgccct
    11161 cgggcctgtg agtaggcacc ccgagacgag gacgcacgcg ccaaagcgaa tgctcactgc
    11221 ccggcggaac ttgcgtggag ctctcattcc tgactctccg tgttcatgaa cgcggtcagc
    11281 cacgcggcgg gatccttccc ctgagctgac atcatccgct gcatgcgcgc gagctggctc
    11341 gcgcgccccg aaatgacagc cagctcggca tcaaagcccc gcagatcgag ttgacacacc
    11401 acgctgtgcg ggccctgctt gatcagaaaa cggcgcgagc cgggctcgag ctgctccttg
    11461 atgagcttga attcccgctg cgtgagtccg aagcccgagt attccgccgg actggcattg
    11521 aggttcggga agaacacttt ggtcggtgtc tgctcgatga tcgtgcgact gatggcactt
    11581 tccagcacat cgcttgcgct ctgcgtcgcg aagcacatca ccccgttcag cttgcgccag
    11641 gtcttcggcg catccttggc gaagctctcg aaagcaggat ccgacagcat ccgccagaac
    11701 tcatccagcc agcagacgag ccgccgacca tccagcagct gtctgacgag atggaagaga
    11761 tagagcgtca ccggagtccg cgtgagctca tgctcgagga actccgtgac atcgaaaccg
    11821 accagcgcac gcgcctccat gcgcgcaacg accgtgtcct cgtcgttgtc gaagacccag
    11881 gcgtagtcgc cccgacttac ctcgcaccag cacgcgagcc tggcatgaat gccctcagga
    11941 tcggtgggat cgaggaactc gatcaacctc gagagtcggc gcgccttgcg ctccagcgcc
    12001 agggttccgc gcagggcctg atccaggtcc gcctgctcgc gggtgctggg cgcaaggctg
    12061 ccaccgaaac gcacgagcgc ccgcaaccag gacttgagga actcgatgtg tgcaggcaga
    12121 gcggggagct gcagcggatt gaaccccgtg ggcgttccgt tgcgcaacgg cagatattcc
    12181 cccccgagtg ccctcaccag gatttccagc ccgcgatcct tgtcgaagat cacctgggtc
    12241 gccccctgct gctccagcat cgcaaccagg aacccgatga acactgtctt gccggagccc
    12301 gtcggaccgc aaatgaaggt atggccggta tccttgggac ctgagggctc accgtccgtc
    12361 gagtggcttg catgcaggga gaagtagtaa ggcgagcgcg cgctcgtcat gagcagcgcc
    12421 agcgcggcgc cccagtgatt gccggtcgca cggccagccg ggtagttgtg aaaggcagcc
    12481 atggcggcga agttgcgtga gctgatcggg gccttgcgcg gccgcatggc aaagttgccc
    12541 ggcagttgtg cccagaatgc ggcctcgagt gccaggtctt cccgggccac ggtcatgccc
    12601 gtatcggcca acagactgcg ggcgagcgcg acatagttgt tgagtgttcc gtgtcggcgc
    12661 gcggcctcgc cttcgcccgg atcgcattgc acctgcagcg aaaagtggtg atctcccatc
    12721 acgaactcgt tgctcgtcaa agcatcgagc gcatccttca gctcctcggc ctgcgagacg
    12781 gcgaagtcgc cggcgttctg catcctgtag aactgtctct gcagaagcgc ctggctggtc
    12841 ggcttggcga gaaaggcgaa ggactgcgtc agcaccatgg gaaagggtgc tgaaagcagc
    12901 cggtcgtaca taccgaccac gctcggcgtc gggtactcct tgatgcccag catggcgcca
    12961 agccgagtac gggtcggtgt gcggtattcg atggtttccg cgccgaaaaa caggcgcgtg
    13021 gtggcgatcg cctcgctcac cgcgcagcgc ggcagcggca tccgttgcca ttcgccattg
    13081 accagcaatc cgaggaattc aaggatcgag gagtaccaca cctcacccag gcgataggtc
    13141 ccgagggtct ccgggtcgta gcgtgccaag gaagcgcgca gcgtctgacc gagcttctca
    13201 caggcatcga gcgcgtcggc gagctcgagc ctgggggcat cgcgttgggc acgggcaagc
    13261 gctcgcgaca ggattcccgt ggtgacgccg gcagccggcc tgtacagggc ggcgagaaac
    13321 acttcgttga tcatgagcgt ctcagccgcg aggcgccgcg catactttgc ggcaagcctt
    13381 ccagcgaacc cgcccggacc ttcgcctggc tcgggcgtcc gttccctgcg acggatcacg
    13441 tgcgtccaca gggcgacatt agggctcgca atgttcctcc acagcacgtt caatctttcg
    13501 tgccaggtgt tgacttgacc gtcatccgcg gtttcaaagc tcgcgccact gagcttcatg
    13561 acttgcagat agtcgcctga tacggtcctc agcacgtgtg gggcgacatg cgccgcacac
    13621 ggaatgcgca cggccgccgc atcctcacgt cgcatcgcgc tcatacgcct gccgctcaac
    13681 atgcgcgcac ctggtagcac acgcgcaggg tggcaagatc gagatcgcgg cggcgacgcg
    13741 caccccgaac ggagggtata tcgagctcca gcgggctata gctgctcgcc ttccagtagc
    13801 gcaggcttcc caacagggcg ggcagacgcg tacgcagcca caacatcgcg aggtcgaaga
    13861 ttcgcgcatc gcgggcacac agcaaggcac agatggcatg aatcggaatc gaggccagca
    13921 gagccagcag gttccgggtc aacagaaaca actccatggt aaaaaccagg ttgaaaagca
    13981 gcgctgagta cgtcactccc cagcgcatcg ggggccgggt tgcgcccacg aagagtggat
    14041 cggcgatcag ggcggagctg ctgccggaca tgaccgcctc aaacaccgaa cattccgcgc
    14101 acccaggaaa cgatctgcgg cgcgccgaag gcgatcgcga tgccgaggat cgcaccgatg
    14161 caccagcccc aggacatccg gttcgccatc gccatggccc cgagcaccat gatgaggatg
    14221 atggcgatgg gggtgagcca ggccaggatg ttgttctgca gggaggtggc accggtgagg
    14281 aagggcgagg cctggccgaa ggccagcgcc ggcgcgccag ccagagcgac agccagcgcc
    14341 aggctgcacc cgcggacatt tgtggggcgg agtttcatcg ggaccacctt caagttgagc
    14401 gagcaggatt catcgcatac tggcgacgaa tgtcggccga gaagctcttt cgagcgcagg
    14461 tcgatcaggc ctccgcctcc agaacgacgc gtccgtcctc ctcgtcctgc tgggcatcga
    14521 actcctgacg cttcgcgcgg cccggggctc cgaagcgaaa gccctgaacg atgaaggagt
    14581 ggtcgtattg cttttcgccc tgcttgtcgg tccagttatg ggagcggatg cgggcctcga
    14641 gaacgagctg atcgcccttg cgcgcgttct tcgccagaga ttcccccagg gcaccgaaag
    14701 ccacgaacca caggctcgtg atgacttcgc gggcggcgcc ctcctcgtcc ttgccggcat
    14761 aatcattgcc gaccaggcag aagcgcgtgt acatggtgtc gcctttggcg acgagctcgg
    14821 ggtttcgggc aagattaccg atggcaagca acgtgaagct gttcatcgcg gatcctcctt
    14881 gatcgctcgg ggcgccctgg cgggcaaaag gggcggcgag cgggcgacag tgtagacgtg
    14941 cggtcggggg cgggaaggcg gattgcttgt gtgtttgcgc agaaattcgc acctcggcgc
    15001 gtgtatcctt cactcaccat tcgcaacgag aatcttcccg ccaagctcat gatggccgat
    15061 gcccgtagtg taattccgag cctgctcgcc cgcctgacgg gtcccgtgtt ggcaagtctg
    15121 tttctggctg cctgtgccaa cgtcccggtg gtcgatgaaa ccctcagcag cacctactcg
    15181 ccgtcggaaa tcaagatcat gggcgcgcgg ggccccctgt cggccaggca gtcgcaggcg
    15241 gttctcaagc atctcgcggc ccaggccccc gatgccggag ctctcgagcg ccatctcgca
    15301 atcgagcaag ccgtcgcgga aagtccgctg tttaccggca atcgcgtgca gatcctgcgc
    15361 gatggctctc agacctttcc ggccatgttc gaggccatcc attctgccca gcactacctg
    15421 cttctcgagt actacatttt cgaagacgtc acctgcaacg gcgagcacct cggcgacctg
    15481 ctggtcgaga aggccaaggc aggcgtgaag gtcaatgtga tctacgacgg catcggctcg
    15541 atcggcacgc cgtcttcatt tttcgatcgc ctgaaggaag ccggcgtcca attgctcgag
    15601 ttcaatccac cgaatccgtt gcgctcggtg cgccacttct cggtcaacga ccgtgatcat
    15661 cgcaagatgc tgatcgccga cggcaacgtc gtgatggtgg gcggagtaaa cctcagctcc
    15721 acctacgaga gcggccccag cggctcggac gccgggtccg gagagaagca gaagaaagcc
    15781 gacaaggcgg ccccggaggt ctggcacgac acggatctgg agatctgggg gccggtcgtc
    15841 atcgagctgg agaagctctt cagggaacac tggaacgagc agaagggcac accgctcgac
    15901 atcaacctgc agcctcccgg cgagcggatg ggcaatgagg tcgtgcggat catcggcagc
    15961 gcgccacggc gcctgaggac acgttattac gtcaccgtat tgactgccat ccgcagcgcc
    16021 gaaaacagca tctggatcac ctcagcctac ttcgtgccca ctcaccagga aaaacgcgac
    16081 ctgatccgag ctgcacgcaa aggagtggac gtacggctgc tgctgccctc gcgcagcgac
    16141 tcctccgcgg cgctcgcggt ccagcattcg gagtattcgg acctgctgcg tgccggcgtg
    16201 aagatcttcg agcgcgacga tggcatcctg cattccaaga ccatggtgat cgatggagtg
    16261 tggtcgatta caggctcctc caactttgac catcgcagca tccttttcaa cgatgaggtc
    16321 gatgccgtgg tcatcggcaa gaacaccggc gaggaactcg ccaagctctt tcagagcgat
    16381 ctcgagcacg ccaagccgat cgacctcgcc agctggcgca agcgctcgac gctcaccaag
    16441 ttgcgcgagc agttctggcg tctctgggag aatcttcttt agtgtgctct aaatgcgcgg
    16501 gatcgcacga ttgcgctgcg ctcctgctgc gcgcaagccc tgggcgcacc tccgtgtgcg
    16561 ctggctcggc gccatgcgcc gcgcccctgg tgcgcccctc gcgcgagctt acggccgatt
    16621 gttgggtcga tcctgccgat tgttaggccg atccggcggg ttgcttcggc gctcctcagc
    16681 tcgggctggc ggaggtggcg ggcgccgctc gggcgctgga cgggcggcgc ctggtggcgc
    16741 cgcgggatgc gcctgcgggc tacccggccg aggcgactcg attcgcggcg ccggagccgc
    16801 agacggcggt gtgaccgcgc ggggtgcttc aggctcaatg gtacggtcct gcggcgggtg
    16861 cgcctccggc aatgggtgcg cttccggcaa tgggtgcgct tccggcaatg ggcgcgcttc
    16921 cggcaatggg cgcgcccgcg gcaacgggtg cgcctgcggc aacgggtgcg cctgtggaat
    16981 cgggcgaccc gactcgcggt tggaagagga ctccggcggc gcggcactgc gctgcgcccc
    17041 aggcgggcgg tcaccccgca ccgcattctg tggcggcggc acgggcgtgg cctgcagcgc
    17101 gcgctcgcgc tgctgcaggc tcggtgccgg agcgctgcgc accgcaggca ggctgtggac
    17161 cgggcccggt gtgacgacgc ggacgcggct cgttgggacg cgctcctgta tcgtcgcaag
    17221 ttgcggaccg gccagcgggc ggccgccgtt cgcgcgaatc gcttcctgtt gtttcgcgaa
    17281 ggacaccggt gcaggtggcg gggccacgcg ggcaaccact ggccggttcg agaacgccgc
    17341 cggcggacgg tggacatcgc gaccggcaac actccccagc acgctctgcc tctctggagc
    17401 gatcgccgga gcggcaccaa cgggtcgcat acggccaagc tcggcgggcg cgattgctac
    17461 ggtgtgcccg cgcacgggct gcgcggagac gaaggtcgat ctcgagaccg cggtaattgc
    17521 gccaggcgca tgctggttca cataggtgat gttggtgacc ttgttctcat acacgttggt
    17581 gatgtacgtg ttgttcacga tcgtcgtgtt ggaaacgttc acattgcgaa cgtaggctgg
    17641 actgacgtgg tacccgggga cgtacacctc gcgcggtcca agcgcgaacc aggccaccgc
    17701 cgcgacaccg gccccgacgg cgacagtcgt ggcaacaccg cccggggtgc cgacccaggc
    17761 caccagcgcc ggggcgtaca cgtgccgcac gtggcgcgga ccgggaaccc agaaccagcg
    17821 cccacctgca tttgcccacc ggccgtaatg aaacggcgcg aacccccagg gagcgttatc
    17881 cacccaggtc catccccagg gagagatcca gacccagcgg ccgtcctgat acggtgccca
    17941 acccggccca acatcgttcg gcgtccagac agagccgtat tcgggaacgg agctccagga
    18001 gccgtactgg ttcagctgct cggcgccggc catgtccggg ggcacgtact ggctggcctg
    18061 tgcttgcgcc tgctcttcct ggtgctcacg atccatggac cactggtcga gtgcatcggg
    18121 tgcgcccact gagctggcat cggctgagac ctgatccgtg ccggtgaagg tcactgcctg
    18181 ttgggtgccg atcgggatgg tctgcccggt tgcgctcgcc tccgcctgcc cattgctgac
    18241 cgcgacgagt gtcgtgtcgc ctgcctcatt cacctcgacc cgataatctc caggtgcctg
    18301 caaggtcagt gcgatgttcg gcgtatcgat ctcgaaggcc tggttttcgc ccatgtcgcg
    18361 cacgtgcacg attgccgtgc ccgcagtcac attcatctgg gccatgcgat cgtcgagatt
    18421 caggaacgag aaaccggtcg tgctgcccag gcggattgcg gctgagccca gatcgagctc
    18481 ggcgcgcgaa ttctgatcgg tccagagctt gtcgccggtc gtcagcgggc ggttgggagc
    18541 cgccgccgcc cagtcctgta cgccggccgg ttgcaacgac acggaaccct ccgcatcgct
    18601 gaggcgcgcc actcgccccg gtgggtcgcc ttcatcggcg ccggcgaccg ccatggcggc
    18661 ggcggccagc accaggaagg cagcgatggc gatcggatga cgtgagaaga gtctgcgata
    18721 acccatgatg tgcgctccac cctgatcggc tcatcaggtg actaccttaa tacaacgccc
    18781 gaggcgatga accgggaatg aacgcggcgc gtcggagcct cgccgagggc ggcaacccct
    18841 aagtggccgg aaacgagctc tccggcggtg ggggctgcgc gagcttgcgg gcggcggtgc
    18901 gcggacggta gcgcctccaa tgcagcagag cgaacaggag gcccgcggca cccagcatct
    18961 cgtgagccgc agcagcgatg tcgtgcaagc catccgtcgt gtaatagagg gcatagccgc
    19021 tgagtacgag cacgacagcc acgccgacaa cagccaaccc ggaaggtcgc ttgatcgtct
    19081 ggccccaccg atcactgacg tgtcgcgcgg tgatccatcc gagcaggaac acggcggtca
    19141 cggcgatcca gccgtgcagg cgcagaacgg ccggctccca gggattcggg agcggcccga
    19201 agtctcccgg gtgcgtaaaa aactcatgca gcagcagcca caggcagcca ctcacccaca
    19261 gtgcgccgaa gacgatgtag atcgccatac gcaatccggc gcgcatcggt gttggcaggt
    19321 tggcagccat ggctgtcagc tctccggggc gccagcggtg cgcagcgcaa cctgccagag
    19381 tgccagcacc cagcgcgcac cctgggtcac atgctcgcag gacagcgtgg cgccggcgat
    19441 gttcttgatc tccgtgccga ggcgcagcgc aggcagatcc tgccgaccgt cgaactgctc
    19501 ccgccaggcc ttgccgcgga tctcgccacc gtggctctca cgataggaga ggacctccag
    19561 tgcactcatg cgccccgccc gatcgatcgc gacggcgtag gtgatcatgt cctgtctgcc
    19621 cagcacctca tcgacgaaaa cgtgcccgag cagatcgcca ccgtgaaagg cccgccaggc
    19681 acgcaacgag cgatgcggcg gctgagggcc ggcgagctgc gcgacttgtt gcttctgcgc
    19741 aggcgtgagg gccaggacga tctcctcgaa gcgatccgcc tgagagaaca gctcgtgctg
    19801 cgcgtccgcg acgctgaggt aatcggcagc ctccaccacc tgggttggcg cggcgccgaa
    19861 actgactagc gctgcgcccc cgagcagcgc caggctgtgc tcacacaagt acgctgcatg
    19921 cagtagtgcg cgcggcctgg ccccgatcgt gtgatttgcg cggctcctgg cggacatgat
    19981 ggaatcttga caataatgca aatgcgaatc attcttactc gctacagcat agcgaaccgg
    20041 tgggcgctgc gccagcggaa gcttggcccc ccatgcgcca tgcagggcaa gcccgaatga
    20101 attacatgca agcaccggcc gaccctgtgc ccccccaccc cgagacggcg aaggtccgcc
    20161 ggatgcgtgc cctcctcggc acgttcgtgg tcatcgaagc gactgcgccc tgcgtcgagg
    20221 ctgcgcactc ggccatacgc gcagcgtttg cgaccatcgc agagttgcag actctcttgc
    20281 accccggcac ggcgcggggc gacctgactc ggatccgtga ggcttcgccc gggacgccgg
    20341 ttagggtggc ggcgagcacc tgggaccttc tgcggctggc gagtgagctc agcgcactga
    20401 gcgagggagt gttcgatccc agcctgcccc atcgtcccgg aaagttgcgg gacctggagc
    20461 tgctggccga gcatcgcatc ctcgcccatg ttccggtggc gctcgacttc ggcggcttcg
    20521 cgaaaggcca tgcagtcgat cgttcgatcc aggtgctggt gagtcatggg tgccgcagcg
    20581 ggctggtgaa tgcgggaggg gacatgcgcg cgtttggtgc ggatccgtgg cccgtggcgc
    20641 tgcgcggatc ggacggcaac tttaagacct tcgagctcgc ggatgcagcg cttgcggtca
    20701 gcgacagcag ctcgcaagag gcgccgtccg agcaccaggg ctactacaac cgcgcatggc
    20761 cgcaggacgg actcatctgc acccgagctg ccgtcgtcgc ccgggaggcg gtgctcgccg
    20821 atgccctcac caaatgtctg atgttatgtc ggcaagcggt tgctgcgcgc gtgttgcagg
    20881 cattcgaggc cttcgcgctc ccgctcacat agtccgttcg gtgggcgagc gcgcatggcc
    20941 gagaacctct tccggcggct cgccggcatc gggcagctct tcctccatcc ccacggaatc
    21001 cggcgaagcg gccgcgccat catcgagcac gaagccgccc gcctgacggc tccagaggcg
    21061 gtaataggcc ccaccgcgcg ccaggagctg cgtgtgcgtg ccatcctcga cgattcgacc
    21121 ctcatcgaac accaggatcc gatcgaggtg cgcgatggtc gagagccggt gtgccaccac
    21181 gatcaccgtc ttgcctcgca tcgcatcatc gagcgtctcc tggatcgcct gttcggtgat
    21241 cgagtcgagg ctggaagtcg cctcgtcgag aatcaggatc ggggcattct tgaccacgac
    21301 ccgcgcgatg gcgatccgct gacgctggcc gcccgagagc ttcacaccgc gttcgccgac
    21361 caacgaggcg tactggtccc ggatctgcag aatgaagtcg tgcgcatgcg ccttgcgggc
    21421 cgcctcgacg acc
//
LOCUS       DASRHN010000332         6355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1763032, whole genome shotgun sequence.
ACCESSION   DASRHN010000332 DASRHN010000000
VERSION     DASRHN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763032"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFB37_13285"
     CDS             complement(<1..228)
                     /locus_tag="VFB37_13285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO23470.1"
                     /translation="MTVTRSLWSATATPGPVLGPLMTDAQADVVIVGGGYTGLSAALH
                     LSEAGRDVVLLEAAEVGEGASGLNGGQVIPGL"
     gene            complement(372..2309)
                     /locus_tag="VFB37_13290"
     CDS             complement(372..2309)
                     /locus_tag="VFB37_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HZO23471.1"
                     /translation="MSDERTRPLDRSLGEDRPIARRDFLQGALVAAATTLSGPLLKAY
                     GAEMGISTAAQDAPGYYPPLLNGMRGSHPGSFEAAHALRDGQPVGNSTETGEEYDLIV
                     VGGGISGLAAAHFFRARTSPKSRILILDNHDDFGGHAKRNEFELGGRLHLLNGGTLSI
                     DSPRPYAPAPAGLIRELGIDVEGLSRKVEHRKFYEDLGLKSGVFFDRETFGADKLIAG
                     FGGGVPWAKLLADAPLPEKARGDIVQIEEGSVDYLPGLSSDEKKLRLCRISYSDFLRT
                     LVKVDPATIRFYQTHTHGEWGVGIDAVSALDCWGFGMPGFKGMNLAHGSISRMGPTPA
                     GYEDTGGSPTLHFPDGNATIARLLIRNLIPAAIPGATVEDLITARANYHELDQPSARV
                     RVRLNSTVLRVRHVGDPASAREVEVTYARGDKRFSARGRNCVLACYNMMIPYLCPELP
                     DSQKRALHSLVKTPLVYTSVALRNWRSFQKLGIHRVMAPGGYHSSFMLNWKVDIGAYR
                     GSDSPAEPILVHMTRTPCKPGLPEHEQNKAGRAELLVTPFSTFEHNIREQLARTLSAG
                     GFDPARDIEAITVNRWPHGYAPEYNPLFEPLLPPEQRPNVLGRAQFGRITIANSDAGG
                     AAYTDSAILQADRAVTELLHG"
     gene            2391..3362
                     /locus_tag="VFB37_13295"
     CDS             2391..3362
                     /locus_tag="VFB37_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZO23472.1"
                     /translation="MSAKILPPHIRRVLGVCAVLALVLLWPTPASGGAAWDAGIVVGY
                     IAAAVALVLYLYPLRGAGLPGKALPHRRLLTVSQHRRLGWVALCLAAVHALILFAVEP
                     LTFHYALPSAPLYMLCGIGALAALALLVPTGLSSRSVLRHTPGRSGVGLWHAVLAALF
                     VGLLGAHLVGSGQLIDTLPKQFSLCILLALPVFWAVLRPRNELMRAGHWATLVLSFAL
                     AAALLLLPMRPSAARLLEPATRPSGMLPVHFPHELHMSVNCVVCHHNFRDATGTANCI
                     DCHRSRREDLPHSSEQTFHIFCRQCHHDLALEGRKHGPTRACQSCHH"
     gene            3505..4659
                     /locus_tag="VFB37_13300"
     CDS             3505..4659
                     /locus_tag="VFB37_13300"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HZO23473.1"
                     /translation="MRPFLSGVMALALLGSTCLVAAPTPAPLPAEQLTVEKLGHMSPH
                     WVYVFDEALNNEIDMRVLAYDGDTYKRLGQIGAGFMAGVNLSPDGKTTVVATSYFSRG
                     GHGTRTDVVEFNDNSTLAKTGEIILPAKHAQTFPMLYDVAYSSDARFLYASYLTPAAS
                     FGVLDPVRKTVLGEIDTAGCVLVIPSGPNHVSSICESGRLLTVTLDAKGHEASRATSE
                     PFFDPDRDPIFVQAIPTGGGYAFLSFLGQVHEVDFSQGPPAFRPVWSLVSATEKGAWR
                     PGSVQIGAIQRALGRLYVSMHQGGEGSHKDYGTEIWVFDLKTHQRVARWAFDAKNWGA
                     ITALQVSQDRAPLLFAATDHADLVILDAQSGKAKHVETQLGQNPWLLMSP"
     gene            4669..5187
                     /locus_tag="VFB37_13305"
     CDS             4669..5187
                     /locus_tag="VFB37_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HZO23474.1"
                     /translation="MHPLDRAFESSARSLAKRTSRRSLLASLGRMLTGAALLPLLPID
                     RTARAQSAGAAQPDINDARSCDYWKYCAIDGFLCSCCGGSAHSCPPGTQPSPITWIGT
                     CHNPNDDRHYIVSYNDCCGKVSCGSCFCNRNENEKPMYRLSRNNDVNWCMANTEGDYH
                     CSVSVILGVAEK"
     gene            5294..5638
                     /locus_tag="VFB37_13310"
     CDS             5294..5638
                     /locus_tag="VFB37_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23475.1"
                     /translation="MLYCMGCHGTAAQGVPGKVPALAGTLGRFMRSAAGRDYVLRVPG
                     AANSALSDAQLAAVLNWVARTYDAAELSSRTPPFTAAEVTRVRRAPLASVQATRSAVV
                     KSLEAAAAPPPP"
     gene            complement(5654..>6355)
                     /locus_tag="VFB37_13315"
     CDS             complement(5654..>6355)
                     /locus_tag="VFB37_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23476.1"
                     /translation="NSDTVDSYEIGAKNNIQNRVRIATSIYYIKWHNIQQTVVPPICQ
                     ISFISNLGEATAKGGDIQAEIDLAGGLSAELAAGYTEARYTRDSRFPGQTTAPIVIKG
                     DSIIGESGQPNAPVTAAVGLEYKFALLEHESFIRADYEFEGRNKWTSPQQDAASAQFD
                     AEFFTLPSTSFVSMRAGMTLGQWSVEPFVDNLFDAHPITNYDFSIDYGAGVNRLMRGY
                     TFRPRTIGITAMYRY"
BASE COUNT         1070 a         2073 c         2031 g         1181 t
ORIGIN      
        1 cagcccgggg atgacctggc cgccgttgag acctgaggcg ccctcgccca cctccgcagc
       61 ttccaggagc accacgtccc gaccggcttc gctcagatgc agcgcggccg agagaccggt
      121 gtagcccccg ccgacgatca ccacatccgc ctgggcatcg gtcatgagcg gaccgagcac
      181 cgggccgggg gttgcggtgg cggaccagag agagcgcgtc accgtcatcg gcagctgagc
      241 tcgataatcg ataacgaggt atcggttgcg ccagactcta aggcagcccg ctgcaggcgg
      301 tcaacggaca gcagcgcggc tgggcaaggg tcggtgcccg cacccggggg caatccgccg
      361 gagcgaatcc gtcagccgtg cagaagctcc gtgacggcgc gatcggcttg cagaatcgct
      421 gagtcggtat aggcggcacc gccagcatcg gagttggcga tggtgatgcg gccgaattgc
      481 gcccgcccga ggacattggg ccgctgctcc gggggcagca acggctcgaa cagcgggttg
      541 tactcgggtg cgtagccatg cggccagcga ttcacggtga tcgcctcgat gtcacgcgcc
      601 ggatcgaagc cgcctgcgga gagggtccgt gcgagctgct cgcggatgtt gtgttcgaac
      661 gtcgagaagg gggtgaccag cagctccgcg cggcctgcct tgttctgctc gtgctcgggc
      721 agcccgggct tgcagggcgt gcgcgtcatg tgcacgagga tcggctcggc cggcgaatcc
      781 gagccgcggt acgcaccgat gtccactttc cagttcagca tgaagctcga gtgatagccg
      841 cccggtgcca tgacccgatg aatgcccagc ttctgaaacg agcgccagtt gcgcagcgcc
      901 acactggtgt agaccagagg cgtcttcacc aggctgtgca gcgcccgctt ctggctgtcc
      961 ggcagctccg ggcacaggta gggaatcatc atgttgtagc aggccagcac gcagttgcga
     1021 ccgcgagcgc tgaagcgctt gtcaccgcgc gcgtacgtga cttcaacctc acgcgccgat
     1081 gccggatcgc cgacgtgccg cacgcgcagc accgtgctgt tgagacgaac gcgcacgcgc
     1141 gcagagggtt ggtcgagctc gtgatagttc gctcgggcag tgatcagatc ctcgacggtg
     1201 gcgcccggga tcgcggccgg gatgaggttg cggatcaaga gccgcgcgat agtcgcattg
     1261 ccgtcaggga agtgcagcgt cggggagccg cccgtgtcct catagcctgc aggggtcggg
     1321 cccatccggg aaatcgagcc atgcgcgaga ttcatgccct tgaagcccgg catgccgaag
     1381 ccccagcagt ccaaagccga aacggcgtcg atgcccacgc cccattcgcc gtgcgtgtgg
     1441 gtctggtaaa agcgtatggt ggcgggatcg actttcacca gcgtgcgcag gaagtcactg
     1501 tagctgatcc tgcacaggcg caatttcttc tcatcggagc tgagacccgg gagatagtcg
     1561 accgagccct cctcgatctg cacgatgtcg ccgcgggcct tttccggcag cggggcatcg
     1621 gccagcagct ttgcccaagg cacgcccccg ccaaagcctg ctatgagctt gtcggctccg
     1681 aaggtctcgc ggtcgaagaa cacgccggac ttcagcccga gatcctcgta gaacttgcgg
     1741 tgctccacct tgcggctgag gccctcgacg tcgattccga gctcgcgaat cagcccggcc
     1801 ggcgccggtg catagggtcg tggactatca atcgatagcg tgccgccatt cagcagatgc
     1861 aggcgaccgc cgagctcgaa ctcgtttcgc ttggcgtgcc cgccaaagtc atcgtgattg
     1921 tccaggatca ggatgcggct cttcggtgag gttcgcgcgc ggaagaagtg tgccgccgcc
     1981 aagccgctga tcccgccgcc gacgacgatg aggtcatact cctcgcccgt ctcggtgctg
     2041 ttgcccacag gttggccatc gcgtaaggca tgcgccgcct cgaaggatcc ggggtggctg
     2101 ccgcgcatgc cattgagcag cggcggatag taaccgggtg catcctgtgc agccgtactg
     2161 atccccatct ccgcgccgta ggcctttaac aagggcccgg aaagcgtcgt ggctgcggcg
     2221 accagggcgc cctgaagaaa gtcgcgccgt gcgatgggcc ggtcctcgcc gagactccta
     2281 tccaacggcc gcgtccgctc atctgacatg acgactctcc atcgcgccgg cactcagcac
     2341 gatctttcgt ttaagatacg agatcgaagc tcgcgcgagc aagagagcct ttgagcgcaa
     2401 agatactgcc cccgcacatc cgacgcgtgc tgggcgtctg cgccgtgctc gctcttgtgc
     2461 tgctgtggcc gactcctgca tccggtggtg ccgcgtggga tgcaggaatc gtggtgggct
     2521 atatcgcagc cgccgtcgcc cttgttctct atctctaccc gctgcgcggg gctggcctgc
     2581 ccggcaaggc tctaccccat cgccggctgc tcaccgtctc gcagcaccgt cgccttggat
     2641 gggtcgcgct gtgcctggcc gctgtgcatg cgctcatcct gttcgccgtc gagccactca
     2701 cctttcatta tgctttacct tccgcgccgc tctacatgct ttgcggaatc ggcgcactgg
     2761 cagccctcgc gctgctcgta ccaaccggct tgtcgagccg gagcgtgctg cgacacacgc
     2821 cgggccggtc gggtgttgga ctttggcatg cggtgcttgc ggcgctattc gttggactgc
     2881 tgggggccca cctggtcggc agcggccagc tgatcgatac gctgcccaag caattcagtc
     2941 tgtgcatcct gctggcgttg cccgtcttct gggccgtcct gcggccccgc aatgagctca
     3001 tgcgcgccgg ccactgggcg acgctggtcc tctccttcgc tctcgccgct gcactgctgt
     3061 tgctgccaat gcggcccagt gccgcgagac tcctggagcc ggccactcga ccttcgggga
     3121 tgctcccggt gcacttccct cacgagctgc acatgagcgt caattgtgtc gtgtgccacc
     3181 acaactttcg cgacgccacg ggcacggcga actgtatcga ctgtcaccgc agccgccgcg
     3241 aggatctgcc ccactcgagc gagcagactt tccacatctt ctgcaggcag tgtcatcacg
     3301 atctcgccct cgagggtcgc aagcacggcc cgacacgcgc ctgccagagc tgccaccact
     3361 gagtccttca ttggaagaaa tcgccgcatg tggcgatttc gcacacctcc gctccaatgg
     3421 ccggcgctgc gctactttcg actcgcattg cgatgtgcga gactcgatcg gatttcgggg
     3481 cgacggcata aaaggggggg ctcgatgcgt ccattcctgt caggcgtgat ggccctggcg
     3541 cttctgggga gcacctgtct tgtcgcggca ccgacgcccg cgccgctgcc tgctgagcaa
     3601 ctgaccgtcg agaagctcgg ccacatgagc ccgcattggg tttacgtgtt cgatgaagcc
     3661 ctgaacaacg aaatcgacat gcgcgtgctg gcctacgacg gcgacacgta caagcggctc
     3721 gggcagatcg gcgcgggctt catggcaggg gtaaatctat cccccgatgg caaaaccacg
     3781 gtcgtcgcta cgagctactt ttcgcgcggg ggtcatggga cccgcaccga tgtcgtggag
     3841 ttcaacgaca acagcacgct tgcgaagacg ggcgagatca tcctgccggc caagcacgcg
     3901 cagacctttc cgatgctgta cgacgtcgcc tacagctcgg atgcccgctt cctctatgcc
     3961 tcctatctga cacccgcagc ctcctttggc gtgctcgatc cggtgaggaa aacggtcctc
     4021 ggcgagatcg acactgccgg ctgtgtgctg gtgattccgt ccggcccgaa ccacgtgtcc
     4081 tcgatctgcg aaagcggccg gctgctgacc gtgaccctgg atgccaaggg ccatgaagcc
     4141 tcgcgcgcca cctcggagcc gttcttcgat ccggatcgcg atccgatatt cgtgcaggca
     4201 atccccacgg gaggcggtta tgcctttctg tcatttctcg gccaggtcca tgaggtcgac
     4261 ttttcgcagg ggccgccggc ctttcgtccg gtctggtcgc tggtcagtgc gaccgagaaa
     4321 ggcgcgtggc ggccgggcag cgtgcagatc ggtgccatcc agcgcgcgct cggccgactc
     4381 tatgtttcca tgcatcaggg cggcgagggc tcacacaagg actatggcac cgagatctgg
     4441 gtgttcgatc tgaagacgca tcagcgtgtc gcgcgctggg cgttcgatgc gaagaattgg
     4501 ggggctatca ccgcactgca ggtatcgcag gaccgcgcgc ccttgctgtt tgccgccacc
     4561 gaccacgcgg atctcgtgat cctggatgcc cagagcggca aggcaaagca cgtcgaaacc
     4621 cagcttggcc agaacccctg gctgctgatg agcccgtgag gagttgcaat gcatccgctt
     4681 gatcgcgcgt tcgagagctc ggcccgctcg ctggcgaaac gcacctcccg ccgcagcctg
     4741 ctcgcatcgc tcgggcgaat gctgacgggc gcagcgctgc tgccgctgtt gcccatcgat
     4801 cgcaccgccc gcgcacagtc tgcaggcgct gcgcagccgg atatcaacga tgctcgcagc
     4861 tgcgactact ggaagtactg tgccatcgat ggcttcctgt gctcctgctg cgggggcagt
     4921 gcccactcct gtccgccggg aacccagccc tcaccgatca cctggatcgg gacctgccat
     4981 aatccgaatg acgaccggca ttacatcgtc tcgtacaacg actgctgcgg taaggtgagc
     5041 tgcggcagct gcttctgcaa tcgcaacgag aacgagaagc caatgtaccg cctctcacgc
     5101 aacaacgacg tcaactggtg catggcgaac accgaaggcg attaccactg ctcagtgtcc
     5161 gtgatcctgg gcgtagcgga gaagtaacat ccgtgttcca gggaatcgcc ggctcgcgcg
     5221 tagcgcgcgg ctcccgtgcc ttttgctttg ggcggcagcc accggggcac aggcctctcc
     5281 ggtacaggat tacatgctct attgcatggg ctgtcacggt accgccgcgc agggcgtacc
     5341 cggcaaggtg cccgcgctcg cgggtactct gggccgcttc atgcgcagcg cagccgggcg
     5401 tgattacgtg ttgcgtgtcc cgggagctgc gaactcggcg ctttccgatg cccagctggc
     5461 agcggttctc aactgggtcg cccgaaccta cgacgccgcg gagctctcga gccggactcc
     5521 gcccttcacg gccgcagaag taacacgcgt gcgccgcgcg cctctggcat ccgtccaggc
     5581 tacacgcagc gccgtcgtga aaagcctcga agcggcggcg gcaccgccgc caccgtgatc
     5641 tgcggccctt ccctcagtag cggtacatcg ccgtgatacc gatcgtgcgg ggccggaatg
     5701 tatagccacg catcaggcgg ttgacgcccg ctccgtagtc gatcgaaaaa tcgtagttcg
     5761 tgatcgggtg ggcatcgaag agattgtcca cgaacggctc gacggaccat tggccgaggg
     5821 tcatgcccgc gcgcatggag acaaagctgg tcgaggggag tgtgaagaac tccgcatcga
     5881 actgggcgct ggcggcgtcc tgctgcggag aggtccactt gttgcggccc tcgaactcat
     5941 agtccgctcg aatgaacgac tcgtgctcga ggagcgcgaa cttgtactcc aggcccaccg
     6001 cggcagtgac cggagcattg ggctgtccgg attcgccgat gatggaatcg cccttgatca
     6061 cgatcggcgc cgtcgtctgg ccggggaacc gggaatcacg ggtgtagcgc gcttccgtgt
     6121 atccggctgc aagctctgcc gacaatccgc cggcgaggtc gatctccgcc tggatatccc
     6181 cgcccttggc ggtcgcctca ccgaggttcg agatgaagga gatctggcag atcggcggca
     6241 cgacggtctg ctggatattg tgccacttga tgtaatagat gctggtcgcg atgcggaccc
     6301 ggttctggat gttgttcttg gcgccgatct cgtagctgtc taccgtgtcc gagtt
//
LOCUS       DASRHN010000333         7223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_610433, whole genome shotgun sequence.
ACCESSION   DASRHN010000333 DASRHN010000000
VERSION     DASRHN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610433"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFB37_13320"
     CDS             complement(<1..713)
                     /locus_tag="VFB37_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23477.1"
                     /translation="MQWARPKSLSGLMVLGLVLIAVPLLVAIFTAALQIRELADTGQK
                     IVIEGVTGARASQELYSQITSLERTAQLYNVLNDAKLLDLYRQQDARLTVTREQLNQH
                     AAKDARLTLGQVGELQNSIRLMVLSMTAGKGTANMSALSRKFTELSGLVDKVAEQSNA
                     QIDSEVGSLETQTQHARRRLLWQAALLIPLTMVAIFALTVAVGRPLRQLDRAISELGQ
                     GSFTNQIAVSGPHDLERLGG"
     gene            complement(858..1853)
                     /gene="galE"
                     /locus_tag="VFB37_13325"
     CDS             complement(858..1853)
                     /gene="galE"
                     /locus_tag="VFB37_13325"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZO23478.1"
                     /translation="MKRGTILVTGGAGYIGSHVALQLRTRGEQVVVLDDLSRGFRQAV
                     LDTPLIVGKVGDREKVLEVLQTHGVDTVMHFAAYTIVPESVSEPLKYYGNNTCSTRSL
                     LECCVQAGVRNFVFSSTAAVYGIPAGGVAAEDTVTAPINPYGTSKLMSEWMLRDVSAA
                     SSLKYVALRYFNVAGSDTSGRIGQATPKATLLVKVAAEAAVGKRSHVSIFGTDYPTPD
                     GTGIRDYIHVEDLATAHLDALGYLRDGGASTVLNVGYGHGYSVRQVLESVERIAGKRL
                     EIREEPRRAGDPPALIARADRIRSDLGWKPRLDDLDTIVSTALKWERHLQQNPWQ"
     gene            1895..2887
                     /locus_tag="VFB37_13330"
     CDS             1895..2887
                     /locus_tag="VFB37_13330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZO23479.1"
                     /translation="MINKSGAVDVKPFTGLTPEAVLDAASSVGLEVDGRLFALNSYEN
                     RVYQVGRPGGMLVLKFYRPSRWSDAQIEEEHEFTEQLAAAELPVAAPVRIEGHTLFKY
                     QQFRFAAFPWMAGRAPELDAPEARQIFGRTLGRMHQIGALQPFRARPRIGVQRLGWEA
                     REQVLASELLPDYLHERYASVSGALLERVEQVFQDTGNVRDIRIHGDCHMGNLLWNER
                     GPVLVDLDDCAMGPRVQDLWMMLSGSRPEQQRQWAELLEGYQQFADFDFREVRLIEPL
                     RALRMLHHAAWVTHRWLDPAFPRAFPWLGEARYWEGYLRDLLEQVEAVEDPPLG"
     gene            2998..3588
                     /locus_tag="VFB37_13335"
     CDS             2998..3588
                     /locus_tag="VFB37_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23480.1"
                     /translation="MRTSTRTVWLLGIALVLTLTGCGSSSSESDGTAHGKKKFFRTAP
                     THDPASRSPDDMVAAVSAGKPGPVGLKFEIRQSPQAGQPLDVDIAVLPDAPAINRVSA
                     KFQAGEGLELVDGQQLEQVDKPAAGSVIRHVVRVTPKEDGIYTLNATVSVDLAGDSLT
                     RVFSIPVIVGDGMPESTAKAEVAAGPSAAGMNSKAH"
     gene            3604..>7223
                     /locus_tag="VFB37_13340"
     CDS             3604..>7223
                     /locus_tag="VFB37_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZO23481.1"
                     /translation="MSNANTLRSLTNAPETGPGGTTTLVRGEGAVCGEDSPELHAQLR
                     AQLKEARLRSGTEAPDLGVLLRLISAHYDAIDEERRGIVQSMRLMADEARAMAHEARE
                     QSSEHLQVILDHIKDVVLTVDEDGVIQTFNPTGERVFGYVEAEVVGQRIDQLIPKIAV
                     HESITQALHRLAASCGDTHLDLASRELWAQRKGGEFFPAEVAVSKARLSRREMFVLCL
                     RDVTERRESEQAMRESEARYRLLVDHAPEAIVVFDADEDRFVDANEYAEQLFGLERKQ
                     LLQAGVVERSPTLQPDGTPSAELARSYTQEALAGHPQVYEWVLYDGFGKEITCEVRLV
                     RLPSGNKRLVRGSIADISDRKRAERVAAAEREVFAQLTGNAPLTEVLASITRIIEPVG
                     AGAMCSISVLAEDGQKFAYTVAPRVPDCLHRVLEQTSVDIRNGSCAAAVYLGRPVLVA
                     DVGKDPFWEKPRIEALDCGVRSAWATPIKSAEGKILGALGVYRSDPGLPAAAETAIMG
                     HAAQLAGIAIERRLAEQALRSSEAKFRGLFESIAEGVYQSRRDGRLISVNPAFASILG
                     YSTAEELYALPSVATLYWNPADRAEFMRRVESEGEIRNAEFHMRTKDGQQVVILENAR
                     AVRDAEDRITGYEGTIANITERKRAEQAIFAEKERAQVTLQSIGDAVISTDAEGRIEY
                     INPVAETLTAWSLAEARGRPIGEVLNLVNEITREPVENPLVCALGRGEGGMAADHSVL
                     ITRTGQEVAIQESAAPICDRQGRVIGAVIVFHDVTKERRLKRALSYQASHDALTGLIN
                     RREFDNRLHTAVLNAQRGEGAYALLYIDLDQFKVVNDTCGHQAGDRLLRDVTGLLQAR
                     VRASDTIARLGGDEFGVLLEGCTPEQSARIADGVRQAIKEYRFIWGASTLSVGASVGV
                     VQITPETESVAAVMSAADIACYAAKDEGRNRVHVYEADGASHPRHREMHWVARVTRAT
                     EENRLELFFQPIVRVGAADSPPNFHELTIRLRDDDSRLVPPSEFIPAAERYNVMSTID
                     RWVVVRAVELLKRRRESGGPLPMLAVNLSGISLNEQSFAEFILHTVGEPEIASALCFE
                     ITETAAVTSLSNVTFFMRELKARGCKFALDDFGTGLSSFMYLKTLPVDYLKIDGQFIT
                     QIATDAVDRSMVEAISKVGRALGIATVAEWVESEAVLDELKRIGVDFAQGYHLARPAP
                     IA"
BASE COUNT         1274 a         2160 c         2485 g         1304 t
ORIGIN      
        1 cctccgaggc gctcgaggtc gtgcgggccc gagacggcga tctggttggt gaagctgccc
       61 tggccgagct cactgatggc ccggtcgagc tggcgcagcg gccgccccac cgcgacggtc
      121 agggcgaaaa tggccaccat ggtgagcggg atcagcagcg cggcctgcca cagcagccgc
      181 cggcgggcgt gttgggtctg tgtttccagc gagcccactt cggagtcgat ctgggcgttg
      241 ctctgctcgg ccaccttgtc caccaggccg ctgagctcgg tgaacttgcg cgacagggcc
      301 gacatgttgg cggtgccctt gccggcggtc atggaaagga ccatgagtcg aatcgagttc
      361 tgcagctcgc ccacctggcc cagggtgagg cgtgcatcct tggcggcgtg ctgattgagc
      421 tgctcgcggg ttacggtcag acgggcatcc tgctgccgat acaggtccag cagctttgcg
      481 tcattcagga cgttgtagag ctgagcggtg cgctccagag aggtgatctg gctgtagagc
      541 tcctggctgg cccgtgcgcc ggtgacgcct tcaatgacaa tcttctggcc ggtatcggcc
      601 agctcacgaa tctgcagggc agcggtgaag atcgccacca ggagcggcac ggcgatcagg
      661 accagaccga ggaccatgag gccgctcaat gacttggggc gtgcccactg catagccaca
      721 ttctactcac tctgagggcc tttccctcac cgctgctgct cgcgagcgcg cgccgcgcgc
      781 gcaacgagcc tgaaccttgc gcgtgacgac ctcaaaatgt gcggggcgtg ctcacgagcg
      841 gccgagatag cggaaggtca ttgccaggga ttttgctgca gatgccgctc ccacttcagc
      901 gcggtcgaga cgatggtgtc tagatcgtcc agacgtggct tccaaccgag gtcggagcgg
      961 atgcggtcgg cgcgtgcgat cagggcaggg ggatctcccg cccggcgcgg ctcttcccgt
     1021 atctccaggc gtttgccggc gatccgctcg acgctttcca gaacctgtct gacgctatag
     1081 ccatgaccat atccgacgtt cagcaccgtc gaggcgccgc cgtcccgcaa gtaccccaag
     1141 gcgtccaggt gggccgtggc gagatcctcg acgtggatgt agtcgcggat gccagtccca
     1201 tcgggggtcg gataatcggt gccgaagatc gagacgtgac tgcgcttccc cacggctgcc
     1261 tccgcggcca ctttgaccag caaggtggct ttcggagtag cctggccgat gcgaccgctc
     1321 gtgtccgatc cggccacgtt gaagtatctc aacgcgacat atttgagaga ggatgcggcg
     1381 cttacgtcgc gcagcatcca ttccgacatg agcttggagg tgccgtaggg attgatcggc
     1441 gcggtgacgg tgtcttccgc ggcgacgcct cccgcgggga tgccgtagac ggcagcggtc
     1501 gatgagaaga cgaaattgcg caccccagcc tgcacgcagc attccagcag gcttcgcgtg
     1561 gagcacgtgt tgttgccgta gtatttgagc ggctcggaga cggattccgg cacgatggtg
     1621 taggcggcga agtgcatgac ggtatccacg ccatgcgtct ggaggacctc caggaccttc
     1681 tcccggtccc cgaccttgcc cacaatgagc ggggtgtcga gtaccgcctg gcgaaagccg
     1741 cgcgagaggt cgtccaggac gacgacctgc tcacctcggg tgcgaagttg gagcgcgacg
     1801 tgactgccga tgtagcccgc gccgccggtg actaagatgg tgccgcgttt catgaaatga
     1861 acaagcctct ggttggccga aggccataaa caggatgata aacaaaagcg gtgccgtgga
     1921 tgtgaagcct ttcaccggct tgaccccaga agctgtgctc gatgcagcga gctcggtggg
     1981 cctggaggtg gacggccgtc tgttcgcgct caacagctat gagaatcgcg tgtatcaggt
     2041 tgggcgccct ggtggcatgc tcgtgctcaa gttttatcgg ccgtcgcgtt ggtccgatgc
     2101 ccagatcgag gaagagcatg agttcaccga gcagttggcc gccgccgagc tgccggtcgc
     2161 ggctccggtg cggatcgagg gccacaccct cttcaaatac cagcagttcc gttttgccgc
     2221 ctttccctgg atggcgggtc gcgcgccgga gctggatgcg ccggaagcgc ggcagatttt
     2281 cggtaggacg ctcggccgga tgcatcagat tggcgccttg cagccgttca gggcgcgccc
     2341 gaggatcggg gtgcagcggc tcggctggga agcccgggag caggtgttgg cgagcgagtt
     2401 gttgccggac tacctgcacg agcgatatgc ctcggtgagc ggggctctgc tggagcgagt
     2461 cgagcaggtt ttccaggata ccggcaacgt gcgcgatatc cggatccacg gcgactgcca
     2521 catgggaaac ctgctgtgga acgagcgcgg gccagtgctg gtggatctgg acgactgcgc
     2581 catgggccct cgagtgcagg atctgtggat gatgctgtcg ggctcacgcc cggagcagca
     2641 gcgccagtgg gcggagctcc tggagggcta ccagcagttc gcggactttg attttcgcga
     2701 ggtccgcctc atcgagccgc tccgcgccct gcggatgttg caccatgcgg cctgggtgac
     2761 gcaccgctgg ttggatcccg cctttccccg ggcctttccc tggctcgggg aggcccggta
     2821 ctgggagggg tatctgcggg acctgctgga gcaggtggag gcggtggagg atccgccgct
     2881 aggttaatcg gaaaagagtc gcgtgcctca cgaaatgcgc acgcgggacg tgcgtttagc
     2941 ggcatgcaag gggtatcgtc cgggaaacga caaacggagg ggtggcgtag ggaggtcatg
     3001 aggacatcca cacgaacagt ctggttgctg ggtatcgcgc tggttttgac gctgacgggc
     3061 tgcggctcct cctcttccga atcggacggc acggcccacg gtaagaagaa attcttccgc
     3121 actgctccga cccacgatcc cgcctctcga tcgccggatg acatggtcgc cgcggtgagc
     3181 gcgggtaagc cgggcccggt cggcctcaaa ttcgagatcc gacagtcacc gcaggccgga
     3241 cagccgctgg acgtggacat cgcggtcctt ccggacgcgc cggccattaa tcgggtttcg
     3301 gcgaaattcc aggccggcga ggggctggag ctggtcgatg ggcagcagct cgagcaggtg
     3361 gacaaacccg cggcgggctc tgtcatccgc cacgtggtgc gcgtcactcc gaaggaggac
     3421 ggcatctata ctctgaacgc cacggtatcc gtggatctgg ccggcgactc tctcacgcgg
     3481 gtgttctcga ttccggtgat cgtgggcgac gggatgccgg agtcgaccgc gaaagcggaa
     3541 gtcgccgccg gtccctccgc ggcagggatg aattccaagg cgcattgacg ggccgggcgg
     3601 gttatgtcga atgcgaacac gctgcgatcg ctgacgaacg ctcccgaaac aggcccgggc
     3661 ggcaccacta cgctagtgcg tggagagggt gctgtttgcg gtgaggacag cccggagctg
     3721 catgcccaat tacgtgcgca gctcaaagaa gcccgtctgc gaagtggcac ggaggctccg
     3781 gaccttgggg tcctgctgcg gctgatcagc gcgcactacg acgcgatcga tgaggagcgt
     3841 cgcgggatcg tgcagtccat gcgcttgatg gccgatgagg cgcgcgccat ggctcatgag
     3901 gcgcgcgaac agagctccga gcacctgcag gtgatcctcg accatatcaa ggatgtggtg
     3961 ctcacggtgg atgaggacgg tgtcatccag acgttcaatc ccaccggtga gcgggtcttc
     4021 ggctacgtcg aggcggaggt cgtcgggcaa cgcatcgacc agctcattcc gaagattgct
     4081 gtccacgaga gcatcactca ggcattgcac cgactcgcgg cgagctgtgg ggatacccac
     4141 cttgacttgg cttcgcgcga gttgtgggca cagcgcaagg gcggcgagtt ctttccggcg
     4201 gaagtcgccg tgagcaaagc gcgtctgtcg cgccgtgaga tgtttgtcct gtgtttgcgc
     4261 gatgtgaccg agcgccgcga gtcagagcag gccatgcggg agagcgaggc gcgctatcgc
     4321 ctcctcgtcg atcacgcccc agaggcgatc gtggtgttcg atgcggatga agaccgcttc
     4381 gtcgacgcga acgaatatgc ggaacaactc tttggcctcg agcgcaagca actcctacag
     4441 gcgggggtcg tcgagcgcag cccaactctg cagcctgacg gcaccccatc cgcggagctg
     4501 gcgcgcagct atacgcaaga ggcactcgcg ggccacccac aggtgtacga gtgggttttg
     4561 tacgatgggt tcggcaagga gatcacctgc gaggttcgtc tcgtacgctt gccgagcggt
     4621 aacaagcgcc tcgtccgcgg aagcatcgcg gatatttccg atcgcaagcg agctgagcgg
     4681 gtcgcggctg ccgagcgcga ggtgttcgcg cagctcacgg ggaatgcgcc gctcacggag
     4741 gttctcgcgt ccatcacgag gatcatcgag cccgtgggcg cgggtgcgat gtgctccatc
     4801 agcgtgttgg ccgaagacgg ccagaagttt gcctacaccg tcgctccccg tgtgccggat
     4861 tgcctgcacc gggtgctgga gcagacgagc gtcgacatcc gtaatgggtc ctgcgctgcc
     4921 gccgtttatc tcggtcggcc ggttctggtg gcggacgtcg gcaaggatcc tttttgggaa
     4981 aaaccccgca tcgaggcgtt ggactgtggt gtgcggtccg cgtgggcgac acccatcaag
     5041 agcgctgagg ggaagatcct cggcgccctc ggggtctacc gaagcgaccc gggtctgccg
     5101 gccgcggcgg agacggccat catgggacac gccgcgcagc tcgccgggat cgcgatcgag
     5161 cgacgcctgg ccgagcaggc cttgcgcagc agtgaggcca aatttcgtgg gctcttcgag
     5221 agtatcgccg agggggtgta tcagagtcgc cgcgacgggc gtctcatctc ggtgaacccg
     5281 gccttcgcga gcatcctggg ctacagcacg gcggaggaac tctacgcgct cccaagcgtt
     5341 gcaacacttt actggaaccc agcggatcgc gctgagttta tgcgacgtgt ggagtcggaa
     5401 ggggagattc gtaatgccga gtttcacatg cggaccaagg atggtcagca ggttgtgatc
     5461 ctcgagaacg cacgggcggt gcgagatgcc gaggaccgta tcaccggtta cgagggcact
     5521 attgcgaaca tcaccgaacg caagcgcgct gagcaagcga tcttcgccga gaaggagcgc
     5581 gcgcaggtga ccctccaatc gatcggcgat gcggtgatca gcacggatgc ggaaggccgc
     5641 atcgagtaca tcaatcccgt tgccgagacc ctcacggcat ggagtcttgc cgaagcgcgc
     5701 gggcgcccca ttggcgaggt gctgaatctc gtcaacgaga ttacgcgcga gcccgtcgag
     5761 aatccgctcg tctgcgctct cggccgtggc gaaggcggca tggcggccga ccactccgtc
     5821 ctcatcacac gcacgggcca ggaagtggcc atccaggagt cagcggcacc gatctgcgat
     5881 cgccaagggc gtgtgatcgg cgcggtgatc gtgtttcacg acgtgaccaa ggagcgtcgc
     5941 ctcaaacgcg ccctgtcgta ccaggcgagt cacgatgcgc tcaccggtct catcaatcgg
     6001 cgcgagttcg acaaccggct gcacacggcc gtactgaacg cgcaacgggg agagggtgcc
     6061 tacgctcttc tctacatcga tctggatcag ttcaaggtgg tcaacgacac ctgcggccac
     6121 caggcaggtg atcggctgct cagggacgtg acgggacttt tgcaggcgcg agtgcgagcg
     6181 tctgacacga tcgcacgtct cggcggcgac gagtttggag ttctactcga ggggtgcaca
     6241 ccggagcagt cggcgcggat tgccgatggc gtgcgccagg ccatcaagga atatcggttc
     6301 atctggggcg cgagtaccct gtcagtcggc gcgagcgtcg gcgtggtgca aatcaccccg
     6361 gagacggaaa gcgtcgcggc ggtgatgagc gcggcggata tcgcctgcta cgcggccaag
     6421 gatgagggtc gcaaccgggt ccacgtctat gaggcggacg gcgcctcgca cccgcggcat
     6481 cgcgagatgc actgggttgc gcgggttacg cgggccaccg aggaaaatcg gctcgagctc
     6541 ttcttccagc cgatcgtgcg tgttggggcc gccgatagcc cgccgaattt ccacgagctc
     6601 acgatccgct tgcgcgacga tgacagccgg ctcgtcccgc cgagtgagtt catcccggcg
     6661 gccgagcgtt acaatgtcat gtcgaccatc gaccgctggg tcgtggtgcg cgcggtcgag
     6721 ctcctcaagc gccggcgtga gagcggtggg cccttgccca tgctcgcggt gaacttgtcc
     6781 gggatctcgc tcaacgagca gtcgttcgcg gagttcatcc tgcacacggt gggagagccg
     6841 gagatcgcca gcgcgctgtg tttcgagatc acggaaaccg ccgccgtcac cagcctttcc
     6901 aatgtcacct tcttcatgcg cgagctcaag gcacgaggct gcaagttcgc gctcgatgac
     6961 tttggcaccg gactgtcgtc cttcatgtat ttgaagacgc taccggtcga ttatctgaag
     7021 atcgatggac agttcatcac gcagatcgcc accgatgcgg tggatcgaag catggtggag
     7081 gccatcagca aggtggggcg ggctcttggc atcgccacgg tggccgagtg ggtcgaaagc
     7141 gaggccgtcc tcgatgagct gaagcgcatc ggggtggatt tcgcgcaggg ctatcacctg
     7201 gctcggccgg cgccgatcgc gga
//
LOCUS       DASRHN010000334        21251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_119620, whole genome shotgun sequence.
ACCESSION   DASRHN010000334 DASRHN010000000
VERSION     DASRHN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21251
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119620"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="VFB37_13345"
     CDS             <1..1764
                     /locus_tag="VFB37_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO23482.1"
                     /translation="ELPILAMEHQYLITEKIPAFEGQKEQLHCVDFDGEIYLREERGA
                     MLMGTYERAGVPWSPIQTPWDFGQNLLPNDLERIAPSLEIGFEHFPVLGTAGIRKVVN
                     GPFTFAPDGNPLVGPIRGLRNFWVACGVMAGFSQGGGVGLALSRWMVDGDPGADVWAM
                     DVSRYGDWATLAYTNARVRENYSRRFRIRFPNEELSAARPLRTTPIYDRLHARHAVFG
                     DYCGLEHPLWFAPSAQEASEKVTFRRSSAHAHVAAECKAVREGVGLLEISNYGKFEVS
                     GPGCVEWLSRVMANRVPAVGRIALTPMLNERGKLIGDFTMCRAGPERIFLIGTYAAET
                     YYMRWFDRHLPPTGVTVRPCAMEYVGLSVAGPKSRALLQSVVREDLKTSNFPFMSFRR
                     VDVGMVPAFIGRVSFTGDLGYEIWVTSDYQRALYDVLMEAGRPHALKLFGSRALHSMR
                     VEKGFGTWAREFRPIYGPHEAGLDRFVDLKKGEFIGRAAALAERESGGKLRLVSFAVE
                     AADADALGDEPIWHENKVVGWVTSGAYGHSVQRSLALGYIPAAVAGETSGFEIEILGE
                     RCKAQRLAAPAFDPEGTRMRA"
     gene            1793..2929
                     /locus_tag="VFB37_13350"
     CDS             1793..2929
                     /locus_tag="VFB37_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZO23483.1"
                     /translation="MATAKSKRAAKSAHAVAKRPRTARAAKSGHGTERYQEPGLLKRL
                     AAGPVICAEGYVFELERRGYLQAGAFVPVVLTEHPEVVEQLHMDFVHAGSDVTQALTY
                     YVHREKLRVIGREKDLEPMNRIALQIAKKVARRTDTLFAGDICNTNIYDPSDRKSLKE
                     VERIFEEQVGWAADAGVDYIVAETFSWGAEALMALEAIRKYSKVPAVVTFSVHRQENM
                     RDGWTPVETCQRLEAAGADVVGINCHRGPDTIMPVLREIRRAVKCEVAALPVPYRTTA
                     AQPSFMSLTDPCCDNARAFPVGLDPFVCTRAEMSKFGQEAYALGIKYLGICCGAGPHH
                     IRALAEGLGRHPQASRYSADMSKHAFFGTDKRIRAVQTEYRTKL"
     gene            complement(3269..3586)
                     /locus_tag="VFB37_13355"
     CDS             complement(3269..3586)
                     /locus_tag="VFB37_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO23484.1"
                     /translation="MMSLHVRCAECTAHRKAAHKGLLWGMFVQPDWRRERLGERLLQA
                     AIDHARVLGGISTLQLSVSEAAGTAQRLYERLGFTVWGVEPDAMQFEGRFVADYHMLL
                     RLS"
     gene            3585..4811
                     /locus_tag="VFB37_13360"
     CDS             3585..4811
                     /locus_tag="VFB37_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO23485.1"
                     /translation="MARTTFINARIFDGSQAEYLEESVVVVEGNRIAEVGRQLPASNA
                     GEVLDVRGCTLMPGLIDAHCHVLGSSVKIAEVETQPLTYVAQHGARMLSHALDCGFTT
                     IRDVGGGEVGIAQAVEHGLIRGPRVLFAGRAMSMTGGHGDFRDPVSSAGACSCCAEGR
                     VSVIVDSKDAMRAAVREELRRGAHCIKLMMSGGVLSPSDPLWMDQFSDEEVLVAVEET
                     ARRRKYVAAHCHPAASIARAARLGVRSIEHATLIDAGSAEAVKSAGAFVVPTLVIVSA
                     LLDSAKTGGLPEWAAEKLRVVSEHSLRGLEMMDRMGLKIGFGTDLLGKLHVQQTREFL
                     LRREVQSPLAILRAATSTNAELLGEATLGRIQAGNEADILIVEGDPLKDIGLLASDGR
                     TLRAIMKAGVFYKRPA"
     gene            4867..5721
                     /locus_tag="VFB37_13365"
     CDS             4867..5721
                     /locus_tag="VFB37_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZO23486.1"
                     /translation="MLRLPLVRLALLAASLVGLLLVLQSVIHALSHQVPRTLHGPVVA
                     GGDLLACVIIVLAYRVEVRWLERRDPAELALDGRIAGLLPGTVLGAGLFCTVCALLGM
                     LGVAGPATWAGTAGLPLAAAGAFAAGVGEEIVFRGVVFRILEGRWGTLAALVFSAALF
                     GFLHGANPGATLISSAAIALEAGVLLGLMYCATRSLWWPIGLHFGWNFTEGGIFGAAV
                     SGGRVHGMLNLPLIGPQLLTGGSFGPEASAIAVLVSLCASAALVYLTVRRGQWRPLRE
                     ATRNHRER"
     gene            5711..6334
                     /locus_tag="VFB37_13370"
     CDS             5711..6334
                     /locus_tag="VFB37_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZO23487.1"
                     /translation="MSAELSALLAFAAASSLLTLIPGLDTALIVRTAVVEGRRSALVA
                     GLGICCGVLTWGVLASTGLGVLFATSRLAYDTVRIIGAVYLLYLGVRLLLSPTSGDAS
                     RPAQQGVGKGAFRRGLFTNLLNPKVGMFYVSFLPQFVPAGTPVAAFGMLLALVHASES
                     FLWFLVLSGASSLARRWLTTGSVQAAINRMTGMVFIAIGLKLMLSRR"
     gene            complement(6385..7614)
                     /locus_tag="VFB37_13375"
     CDS             complement(6385..7614)
                     /locus_tag="VFB37_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO23488.1"
                     /translation="MRIEALSDWTPQQKKVVTAAFLGWTLDAFDYFLVVFVLKDIAHD
                     FGTGVPAVSFALFLTLAMRPLGAFIFGRAADRYGRRPILMLDVVLYSALEFASGFAPD
                     LTVLLVLRALYGIAMGGEWGVGASLTMESIPPRARGIVSGILQAGYPTGYLLASVLYG
                     TLFPIIGWRGMFMVGMLPALLVLYIRRHVPESPHWHRQSAARGGTLTVLRRHWKVGLY
                     AVALMTLFNFFSHGTQDLYPTFLEKQHGLEHRTVGTIAVIYNVGAILGGILFGSLSER
                     IGRKRAIVLAALLSLPVAPFWASAATPISLAVTAFLMQVAVQGAWGVVPAHLNELSPS
                     EARATFPGFVYQLGNLLAAINAPMQSGIAEHLGNNYGLVMGVVAAVVAILIATLTWFG
                     MEAKGISFERASEGVHP"
     gene            complement(7670..8038)
                     /locus_tag="VFB37_13380"
     CDS             complement(7670..8038)
                     /locus_tag="VFB37_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO23489.1"
                     /translation="MIGYVTLGTNDMERAGRFYDDLLGLMGAKRTMQSERYIAWGNDP
                     NAPTLFVIKPADGNKATIGNGVMVALAAKSERDVDRIYARALALGGKDEGPAGSRGEG
                     FYGGYFRDLDGNKLCAFFSS"
     gene            complement(8122..8775)
                     /locus_tag="VFB37_13385"
     CDS             complement(8122..8775)
                     /locus_tag="VFB37_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HZO23490.1"
                     /translation="MLTREQMAMRAAQELRDGQYVNLGIGIPTLVANYIPPGVSVVLQ
                     SENGLLGVGPFPYETEEDADLINAGKQTVTMLPGAALFNSAESFAMIRGGHIDLAILG
                     AMEVSQDGDIANWMIPGKMVKGMGGAMDLVSGARRIVVVMEHSSKDGQSKVRRSCSLP
                     LTGLRVVNRIISDLAVIDVTQTGLELIEVAPGVSADEVKKRTEAVLSVRPDVREMKL"
     gene            complement(8790..9491)
                     /locus_tag="VFB37_13390"
     CDS             complement(8790..9491)
                     /locus_tag="VFB37_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004971542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZO23491.1"
                     /translation="MNKVRSSAAEALKGIRDGATIAAGGFGLCGIPELCIQALRELGV
                     RDLTVASNNCGVDDFGLGLLLANRQIRKMISSYVGENKEFERQFISGQLEVELVPQGT
                     LAERLRAGGAGIPAFFTPTGVGTLVAKDKEVREFDGRKYVLERGIRTDWAIVKAWRAD
                     RSGNLQYRLTAQNFNPLCAMAGQITVAEVDELVEVGDLAPDRIHTPGIYVHHIVVGQH
                     EKRIERRTTRPKVCG"
     gene            9803..11464
                     /locus_tag="VFB37_13395"
     CDS             9803..11464
                     /locus_tag="VFB37_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZO23492.1"
                     /translation="MGPAVSPATFAEAEKLVQIELNTREREEAAANWRNSMAALYERR
                     TGPRRVALDASVAPYSQWNPVLPGSAGIGQRDRFVRSDLDPGAVPKSEAAIAFAPLWK
                     LSRWIESRRLTSEHLTQIYLKRIDRFDPGLRCVITLTRELALAQAKQADQEIAAGTYR
                     GPLHGIPWGAKDLLDTAGIATTYGAEPFRNRVPESDAAVVKRLREAGAVLIAKLSMGA
                     LALNDVWFGGQTMNPWLPQEGSGGSSAGPGAATAAGLVGFAIGSETEGSIVGPTMRCG
                     LTGLRPTFGRVPRSGAMTLCWSLDKLGPMTRSVEDTMLVLQAISGRDAEDLASVPAHL
                     DFDTKESPAGLRVGYFPKWMNEEPATEVDRAMVRHLEELGLHPVEVSIPDWPYDGLNP
                     ILFGESAAAFEDITLSRQIDSLRMQTPDAWPNTFRQSRFVSAVDFVQADRFRRRVAQE
                     MQKLLAQVDLLLVPSLRDEMLTIGNFTGHPCLTLRAGFVHVTEARSDWAPDPQHPLPK
                     FKQPRRVPHGITLVGRLFDEGTIARVGLALERKLNVAGEPPPGFQ"
     gene            complement(11495..14623)
                     /locus_tag="VFB37_13400"
     CDS             complement(11495..14623)
                     /locus_tag="VFB37_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZO23493.1"
                     /translation="MSGSEFPSGSAARPRSVMNRIVASSLRQRFLVVLMTCILIGAGW
                     WAFDRLPMDAYPDLSPPMVEIITQWPGHAAEEVERLITVPAERGMNGVPQLTTIRSIS
                     LYGLSDVILTFRNGTDNYFARQLVFERLNELSLPSGVAPGLAPLSAPSGLIYRYVLQS
                     RDRSPMELKTFEDWVVEPQYRSVAGVADDSGFGGGTMQYQVLLDPVKIASVGLSPQQV
                     ESSLTANNDNAGGGFYSEGGQFYYVRGLGRIKTLEDIGNVVVAVHNGNPVLVKDVGRV
                     VIGIAPRLGEFGFEKQDDAVEGVILLRTGEKMQDVLRGVEAKTLELNQEILPKDVKVV
                     PFYDASNLIALTTDVVEQNLLRGMLLVLVVLIFFLYNLRAGLIVAATIPLALLFAFFC
                     LHLQNASANLLSIGAVDFGILVDGAIFMVENIYRQVASRPAGVTLDVMAIIRDAAAEV
                     DRPLFYAVAVIVAGFLPIYVLSGPSGTLFKPMADTMIFALVGSLIVTLTLLPVLCSWF
                     LRHGAQEKRNPAFQAIQSIYIRGLDYCLDRPWATIIASAILLGLSLLLVPSIGAEFMP
                     KLDEGALWVRATMPYTISFDEAAKIAPQVRQVLHRFPEVTTVTSELGRPDDGTDPTGF
                     FNVEFYVGLKPYAQWKGPYRNKAGLIEVVNDKLAAFPGITFNYTQPAEDAVDEAETGL
                     KSALAVKVFGSDLNTLEQKGKAIKQVLQRVRGIRDVTLVQELGQPSLTITIDRQRIAR
                     YGLNVSDINGLIQTAIGGDVATQVVQQEKEFDLVVRLDRQYRDDAQEIGNILVATPSG
                     QQIPLREFADIRVANGAAFIYRENNSRYIGVQFSVEGRDLAGAVGDAMQQVSSHVSMP
                     QGYRLDWGGEYTEYTASRAQLNVILPLTLFLIFLLLFALYNNFKFPFITVVGVLLSAP
                     VGGIVALWITGTPFSVSSGIGFLALFGVSVLTAVVYISYVNELRLAGIPLERAIRDGA
                     ILRLRPIMMTALVAALGLVPAALATGVGTDSQRPFALVIVSGLLARLLISVFLMPALY
                     ALVARPADRLEV"
     gene            complement(14620..15750)
                     /locus_tag="VFB37_13405"
     CDS             complement(14620..15750)
                     /locus_tag="VFB37_13405"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO23494.1"
                     /translation="MHARVLLLALAASLLVGCSRSADPKPDTSPGADGVSLTQEQRTH
                     VNLYKVTPLRYQRTVDASGVVDFDNDQSTSVLAPFSGPVSRLLVPLGAEVTEGEPLAV
                     VESPDFATAMSTYRKAIATARTARRLADLDKDLIQHNGVSQREEDQAQSDATSAEADR
                     DAALRQLVSLHVPTRTITEIQEGRPVSEPGALIRSPISGTLVERLISPGELLQAGTTA
                     CFTVADLSRVWVMAQLFGADLASVSVGDAADIVTGVGANNFSGRVDNISALVDPDTRA
                     VQVRVVADNRARFLKKQMYVRVLIHDHQESTGLLIPAAAVLHDDENLPFVYVAQSDGS
                     FARRHVTLGYRAADKYDVSAGLRAGEQIVVSGGLFLQFMQSQ"
     gene            complement(15794..17290)
                     /locus_tag="VFB37_13410"
     CDS             complement(15794..17290)
                     /locus_tag="VFB37_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO23495.1"
                     /translation="MSQISQRRPPLLLAVLAPLLIAGCAVGPNFKRPAAPDVSTYTEH
                     PLESIAATEHVTGGEPQQFAPGADISADWWTLFHSKALNDLIDHALANNPDLKAAQAA
                     LSVAHENLLAQRGVYYPAVSASFSATRQGQSVQLAPVPNSNTFIYNLFTPQVTVTYVP
                     DVFGLNRRTVESLQAQEQGVRYQMLATYTTLTANVVVTAIQLAGTQLQIDATRELIDL
                     NTNMVQILRYQLEKGYASGVDLATQEAQLAQIRATLPPLVKQLAQQRDQLAVLVGKFP
                     SQPSAGDFELASLKLPEELPVSVPSTLVRQRPDVLQAEANLHDASAKIGIAIANRLPN
                     LLITGNAGSSALSMSQLLSTATDFWSLGATATAPIFQGGTLLHQERAAKAAYQQAAEQ
                     YRSTVLTAFQNVADTLTALEQDGDALNSAAASTDAAKVSLDLAQRQWKAGYANYLSLL
                     NAQQTYQQARISLVQAQASRYADTAALFQALGGGWWNRADLTSNSNGR"
     gene            17806..19194
                     /locus_tag="VFB37_13415"
     CDS             17806..19194
                     /locus_tag="VFB37_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZO23496.1"
                     /translation="MSGCAHYEAKPLAPTRSARAFEERSLASDSVWQSVAPLLGDAGR
                     TWPPAQWDRAQLLAVALAENPELAVMRAATQVALAQQVRAHEWPNPELTLQSEYSRGE
                     RYTWLYGVGFDFLLLAPRQRRIDLEIAHLATLSSAWQLTEKTWQVRRALIEALSDWQQ
                     ARNEAPLLRQLMDSQRHLVELQERRVHAGEDPPSELIAVRVSLIETEQQLAQSRAAAS
                     SAQSAAAAALGMPPAALDGVAIEWQDWGRPPDLDSQEMPALREAALLARADLASAIND
                     YADSELRLQLAIARQYPEFHLDPGYYWDHGVGKLPLNLGLVLPVFNRNRGEIAEARAG
                     RELAGQRMLAVQADIYGSIEAALREDALAEESVAAIDRQQTLADRQSQQAQLGLQAGA
                     VSASERVAAQVVALQAALEGVRARARRQAARNALEDALHAPLSGPEKALGGALLRRPG
                     LAESNLSKESRR"
     gene            19191..20192
                     /locus_tag="VFB37_13420"
     CDS             19191..20192
                     /locus_tag="VFB37_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23497.1"
                     /translation="MRRGLAGSALAALLACGVMTTHAQDTNDRPQAEDTSKAAAESGS
                     AVPTLTPQQRDRADIAVAHPRAANGAQHWSALGQVLDASAIVADFGQLDAAAASERAA
                     DAELQRLQGLYRADAASSLKMVQSAQAEQVRARTQHEVALAACVARWAPIARLPEEQR
                     HQMIQHAASGQHLLVRASLLGRQSIGQLPESAMLQVDGMQVPARVLGITAQGSADLQS
                     AGILLLVSAAPAGLGPGARIPVTLMMAARSGVVVPDAALIYGTHGARVFKQLMGGSGK
                     ETWHYVEVPIDLLERQADGWLVRGVSSADLIVVRGAGALWSLEAANLAAGPQEDDDD"
     gene            20205..>21251
                     /locus_tag="VFB37_13425"
     CDS             20205..>21251
                     /locus_tag="VFB37_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO23498.1"
                     /translation="MLAAIVRISLANPRTLATLAVLMSVLGAASLTRAHFDVFPDFAP
                     PHVFIQTEAPGFDASQVEGLVTRPLESVLGGGADVKTVRSTSSQGLSVIQVVFASRGD
                     PYRQRQVIAERLGEAVPLLPQGVHAPQLSPLSSSVEYLLHFGFTSPTLSPMQLRDIVE
                     WSVKPQILAVPGVAQVQIFGGEVRERQVFIDPQRLAALGVSFEDVVAAVTRNTQLSGG
                     GFVESAAQRIVLRPQSPGIAPEELAQSLVLTRDGVPIRLADIASIQDGPAPRFGAALI
                     DGKPGLLVETATQFGANTLEVSRLLEQKLNEIAPVLLAQGVEYRPALLRPASFIEDAV
                     RELRDSILIGAILVV"
BASE COUNT         3753 a         6667 c         6997 g         3834 t
ORIGIN      
        1 gagctgccca tcctggcgat ggagcatcag tacctcatca ccgagaagat tcccgcgttc
       61 gaaggccaga aagaacagct gcactgtgtc gatttcgacg gcgagatcta tctgcgcgaa
      121 gagcgtggcg cgatgctcat gggcacgtac gagcgtgccg gcgtgccctg gtctcccatc
      181 cagacaccct gggacttcgg gcagaacctg ctgccgaatg acctcgagcg catcgcgccg
      241 agcctcgaga tcggattcga gcattttccc gtgctgggca cagccggcat ccgcaaggtg
      301 gtcaacgggc cgttcacctt cgccccggac ggcaatccac tggtcggacc gattcgcggt
      361 ctgcgcaact tctgggtggc ctgcggcgtc atggcgggat tcagtcaggg cggcggcgtc
      421 ggtctcgcgc tgtcgcgctg gatggtggac ggcgatccgg gtgcggatgt ctgggcgatg
      481 gatgtctcgc ggtatggcga ttgggccacg cttgcgtata ccaacgccag ggtgcgtgaa
      541 aactattcgc ggcgcttccg gattcgcttt ccgaatgagg agctgagtgc ggcgcggccc
      601 ttgcgcacga ctcctattta cgaccggctg catgcacggc atgcggtgtt cggcgattat
      661 tgcggattgg agcatccgct gtggttcgct ccgtctgcac aggaagccag cgagaaggtt
      721 acgtttcgtc gatcgagcgc ccatgcgcac gtcgcagcgg aatgcaaagc cgtgcgcgaa
      781 ggcgtcggac tgctggagat ttccaattac ggcaagttcg aggtgtcggg tccgggttgc
      841 gtcgaatggc tctcgcgtgt gatggccaat cgcgtaccgg cagttggacg cattgcgctg
      901 accccgatgc tcaacgagcg cggcaagctc atcggcgatt tcacgatgtg ccgcgcgggc
      961 cccgagcgca tcttcctcat cggcacatat gccgccgaga catactacat gcgctggttc
     1021 gatcggcacc tgccgcccac cggggttacc gtgcggccct gcgcaatgga atacgtggga
     1081 ctgtcggttg caggcccgaa atcgcgcgcc ctcctgcaaa gtgtggtgcg cgaggatctc
     1141 aagacgagca atttcccgtt catgtctttc cggcgcgtgg acgtggggat ggtgccggca
     1201 ttcatcggcc gcgtctcatt caccggcgac ctgggctatg agatctgggt gacgagcgac
     1261 taccaacgtg ccctctatga tgtgctgatg gaggcgggtc ggccgcacgc tctgaagctt
     1321 ttcggcagcc gtgccctgca ttcgatgcgg gtcgagaagg gcttcggcac ctgggcacgc
     1381 gagttccggc ccatctatgg tccgcacgaa gcgggcctcg atcgcttcgt ggacttgaag
     1441 aagggtgagt tcatcggccg ggcggccgcg cttgccgagc gcgagagcgg tggaaagctg
     1501 cgactcgtca gcttcgccgt ggaggccgcc gatgcggatg cgctcggtga tgagcccatc
     1561 tggcacgaaa acaaggtggt cggatgggtg acctcgggcg cgtatggtca cagcgtgcaa
     1621 cgctcgctcg ctctcggtta cattccggcc gccgtggcgg gggagacgag cggattcgag
     1681 atcgagatcc tgggcgagcg ctgcaaggcg cagcggctgg cggctcccgc attcgatcct
     1741 gagggcacgc gcatgcgtgc atgacgagct caaagcttta ccggagatcc ttatggccac
     1801 tgcaaaatcc aaacgtgccg cgaagagcgc ccacgccgtc gcgaagaggc cccggacagc
     1861 ccgggccgcg aagagcggtc acggaacgga gcgctaccag gagccgggac tgctcaagcg
     1921 cctggcggcg ggccccgtca tctgcgccga aggctacgtt ttcgagctgg aacggcgcgg
     1981 ctatctgcag gcgggcgcct tcgtgccggt cgtgctgacg gagcatccgg aggtcgtcga
     2041 gcagcttcac atggacttcg tccatgccgg ctcggatgtc actcaggcgc tcacctacta
     2101 cgtgcatcgc gagaagctgc gcgtgatcgg cagagagaag gacctggagc ccatgaaccg
     2161 catcgcgctg cagatcgcaa agaaggttgc gcgccggacg gacacgctgt ttgcgggtga
     2221 tatctgcaat accaacatct acgacccgag tgaccgcaag tcactgaagg aagtggagcg
     2281 catcttcgag gagcaggtgg gctgggcggc ggacgccggt gtggactaca tcgtagcgga
     2341 gaccttctcc tggggtgccg aggcgctgat ggccctcgag gcgatccgca agtactcgaa
     2401 ggttcccgct gtcgtgacct tctctgtgca tcgtcaagag aacatgcgcg atggctggac
     2461 gccggtagag acgtgccagc ggctcgaggc ggccggggcg gatgtagtcg gcatcaactg
     2521 ccatcgcggc cctgacacga tcatgccggt cctgcgcgag atccggcgtg cggtgaaatg
     2581 cgaggttgcc gccttgcccg tcccttaccg caccacggcc gcgcagccct cattcatgtc
     2641 tctcaccgat ccctgctgcg acaacgcgcg cgcgtttccg gtcgggctcg atcccttcgt
     2701 ctgcactcgc gccgagatgt cgaagttcgg ccaggaggcc tacgccctcg ggatcaagta
     2761 tctggggatc tgttgcggcg caggcccgca tcatatccgt gcgctggcag aggggctcgg
     2821 ccgccatccg caggccagca gatattccgc tgacatgtcc aagcatgcgt tcttcggtac
     2881 ggataagcgg atcagagccg tgcagaccga gtaccgcacg aagctttgac gggcggtcgc
     2941 cgccgccatc gagccaggtc agcgctgccg tcacaccgtc gtcggcagca gtgcatcctc
     3001 ctgctttcag cttcccggga cgctgtgggg ctgggctgtg ggcctttcgc tcggcgcgac
     3061 gggcgccggg ttaccggtct tggcctgcag gtatgcagtc aacacgccga tcaaaaacat
     3121 ggcgcaactc cagcggtgag acctttatca ttgtccacat cgctaacgcc ggtgtcctcg
     3181 ccaggttgac ccaagggctc tctcccgaaa gggcggtgag ccgggcgtcc gataagggca
     3241 tcaggctgca acacggttgt cgctgcgatc acgacagtcg cagaagcatg tgatagtcgg
     3301 caacgaaacg gccctcgaac tgcatcgcat cgggctctac accccagacc gtgaagccga
     3361 ggcgctcgta gagccgctgc gccgtgcctg ctgcctcact cacactgagc tgcagggtgc
     3421 tgatgccgcc gagaacccgg gcgtggtcga tcgccgcctg cagcaggcgc tcgccgagtc
     3481 gctcgcggcg ccaatccggc tggacgaaca tgccccagag caggccctta tgagccgcct
     3541 tgcggtgcgc ggtacactcg gcgcagcgca catggagcga catcatggca cgcacgacgt
     3601 tcatcaatgc acgcatattc gatggatcgc aagccgagta tctcgaggaa tccgtggtcg
     3661 tcgtcgaggg caatcgcatc gccgaggtcg gccggcaact tccagcctcg aatgcgggag
     3721 aagtcctgga cgtcaggggc tgcacactca tgcccggcct catcgatgcc cactgccatg
     3781 tgctgggctc gagcgtaaag atcgcagagg tcgagacgca gccgttgacg tacgtggctc
     3841 agcacggcgc ccgaatgctg tcgcacgcgc tggattgcgg gttcacgacc atccgcgacg
     3901 tcggcggtgg tgaggtcgga atcgcgcagg ccgtggagca cgggctgatt cgcgggccac
     3961 gggtgctgtt tgccggtcgc gccatgtcga tgaccggcgg ccacggggat tttcgcgacc
     4021 cggtgagctc agcgggcgcc tgcagctgtt gcgccgaggg ccgggtcagc gtgatcgtcg
     4081 atagtaagga tgcgatgcga gcggccgtgc gcgaggagct caggcgcggc gcgcattgca
     4141 tcaagctcat gatgtcgggt ggcgtgctct caccgtcgga tccgttgtgg atggatcagt
     4201 tctccgatga ggaggtgctg gtggcggtgg aggaaacggc ccggcgtcgc aagtacgtcg
     4261 ccgcgcactg tcatccggct gcatcgatcg ccagggccgc ccggctcggc gtgcgcagca
     4321 tcgagcacgc cacgctgatc gacgccggga gcgcagaggc ggtcaagtca gccggtgcct
     4381 tcgtcgttcc gactctcgtg atcgtgagcg ccttgctcga ttccgccaag acgggcggct
     4441 tgcccgagtg ggcggccgag aagctgcgtg tggtcagcga gcattccctg cgcggactgg
     4501 agatgatgga caggatgggc ctcaagattg gcttcggaac cgatctgctg gggaagctgc
     4561 atgtccagca gacacgcgaa tttcttttgc ggcgcgaggt gcaatccccg ctcgccatcc
     4621 tgcgtgccgc gacttccacg aatgccgagc tcctcggtga ggcgacactg ggccggatcc
     4681 aggcgggaaa cgaggcggat atcctgatcg tcgaaggcga ccctctgaag gatatcggcc
     4741 tgctggcctc ggatggccgg accctgcgtg cgatcatgaa agcgggggtg ttttataagc
     4801 gccccgcctg atcgcgagcc gagtccgccg ttgcctgaca caccggggcc aaatctttcg
     4861 cagcgcatgc tgcggctgcc ccttgtccgg ctcgcgctct tggccgccag cctcgtcggc
     4921 ttgctgctcg tgctgcagtc tgtcatccac gctctgtcgc atcaggtgcc acggactctg
     4981 cacggtcctg ttgttgcggg cggcgatctg cttgcgtgcg tgatcatcgt gctggcctac
     5041 agagtcgaag tcagatggct cgagcgccgc gatccggccg agctggcgct cgatggccgc
     5101 atcgcaggct tgctccccgg tacagtgctt ggggcaggac tgttttgcac ggtgtgcgcg
     5161 ctgctgggga tgctgggtgt tgcaggccct gcgacctggg caggcaccgc agggttgcct
     5221 ctcgcggcgg ccggcgcctt cgctgcgggt gtcggcgaag agatcgtctt tcgcggcgta
     5281 gtgtttcgga tcctcgaagg gaggtggggg acgctcgcgg cactggtctt ctcggctgca
     5341 ctattcggtt tcctgcatgg cgcgaatccg ggcgcgacgc tcatcagcag tgccgccatt
     5401 gcgctcgagg cgggcgtcct gctcgggctg atgtattgcg caacgcgcag cttgtggtgg
     5461 ccgatcgggc tgcatttcgg ctggaacttc acggaaggcg ggattttcgg cgccgccgtc
     5521 tccggcggcc gggttcacgg gatgttgaat ctgccgctca tcggtccgca actgctgact
     5581 ggcgggagct tcggccccga ggcttccgcc atcgcagtgc tggtatcact gtgcgcgtcc
     5641 gccgctctgg tctatctcac cgtgcggcgc ggccagtggc ggccgctgcg tgaggcgact
     5701 cggaatcatc gtgagcgctg agctctcggc gctgctggcc ttcgcggcgg cgagctcgct
     5761 gctgaccctc attcccgggc tggacacggc gctcatcgtt cgcacggcgg tggtcgaggg
     5821 gcgtcgcagc gcgctcgtcg caggcctcgg catctgctgc ggagttttga cctggggtgt
     5881 gttggcctcg accggcctgg gtgtgctgtt tgcaacatcg aggctggctt acgacacggt
     5941 gcggatcatc ggggcggtgt acctgctgta tctgggcgtc aggctgcttc tgagtccgac
     6001 gagcggcgat gcctctcgac ccgctcagca gggggtcggc aagggcgcct ttcggcgcgg
     6061 gctcttcacc aatctgttga atccgaaagt gggaatgttc tatgtcagct ttctgccgca
     6121 gttcgttcct gcaggcaccc ccgtcgctgc cttcggcatg ctgctggcac tcgtgcacgc
     6181 gagcgaatcg ttcctgtggt tcctggtgct ttcgggggcg agcagcctcg ccaggcgctg
     6241 gctcactacc ggctcggtgc aggcggcgat caatcgcatg accgggatgg tattcatcgc
     6301 catcggcctc aagctcatgc tttctagacg ttagtaagac ttgctcgcct gggctgcggc
     6361 agatccatgt gcgtgagcgg atactcaggg gtgcaccccc tcgctggcgc gctcgaagga
     6421 gatccctttc gcttccatgc cgaaccaggt gagcgttgca atcagaatgg ccaccaccgc
     6481 ggcgaccacc cccatgacca gcccgtaatt gttgcccaga tgctcggcga tgcccgattg
     6541 catcggcgca ttgatggcgg caagcagatt gccgagctga tagacgaatc ccggaaaagt
     6601 ggcgcgtgcc tcggaggggg acagctcgtt gaggtgtgcc ggcacgacgc cccacgcgcc
     6661 ctggaccgcc acctgcatga gaaatgccgt gacggccagt gagatgggcg tagcggcgct
     6721 tgcccagaac ggtgcgacag gcagcgacag cagagcggcg agaacgatcg cccgcttgcg
     6781 gccgatccgc tcggacagcg agccgaaaag gatgccgccg agaatcgcgc ccacgttgta
     6841 gatgaccgcg atggtaccca cggtgcgatg ctcgaggcca tgctgcttct cgagaaacgt
     6901 cggataaagg tcctgcgtgc cgtggctgaa gaaattgaac agagtcatga gagcgaccgc
     6961 gtacaggccg accttccagt gcctcctcag cactgtcagt gtgccgcccc gtgccgccga
     7021 ttggcggtgc cagtgcggcg attccggcac gtgacgccgg atgtagagca ccagcaacgc
     7081 cggaagcatg ccgaccatga acatcccgcg ccagccgatg atgggaaaca gcgtcccgta
     7141 gaggaccgaa gccagcaaat aaccggttgg gtaaccggcc tgcaggatgc ctgagacgat
     7201 gccgcgtgcg cgcgggggaa tcgattccat ggtgagagac gcgccgactc cccactcacc
     7261 tcccatggca atcccgtaga gcgcccgcag aacgagcagg accgtgagat ccggcgcaaa
     7321 cccgctagcg aactccagcg ctgaatacag caccacatcg agcatcaata tgggacgccg
     7381 cccatagcga tccgcggcac ggccaaagat gaacgcaccc agcggccgca tcgcaagtgt
     7441 gagaaagagg gcgaagctga ccgcgggaac cccggtgccg aagtcgtgcg cgatgtcctt
     7501 gaggacgaac acgaccagga agtagtcgaa cgcatcgagc gtccagccga ggaaagcggc
     7561 cgtgacgact ttcttctgct ggggcgtcca gtccgacagg gcttcgattc tcaagcctca
     7621 ccgttgcagg gtcgccctac gatgcagcaa gcagctgaga ccgaagcggt cagctggaga
     7681 aaaacgcgca gagcttgttg ccgtcgagat cgcggaaata gccgccgtag aatccctcgc
     7741 cgcgcgagcc cgccggacct tcgtccttgc cacccagcgc aagcgcgcgg gcgtaaatcc
     7801 tgtccacgtc ccgctcgctt tttgccgcga gcgccaccat gacgccgttg ccgatcgtgg
     7861 ctttgttgcc atccgccggc ttgatcacga agagcgtcgg cgcattggga tcattgcccc
     7921 aggcgatgta tctctctgac tgcatggtcc gcttggcgcc catgaggccg agaagatcgt
     7981 catagaagcg tccggcccgc tccatgtcgt tcgtgccgag ggtgacgtaa ccgatcatga
     8041 gagctccttc ttattggttc gactaacaga aactgcggcg catgatagcg cacgcgggcg
     8101 ttggactgca gccgctgagc ctcagagttt catctcgcgc acatccgggc gcacgctgag
     8161 aaccgcctcg gtgcgcttct tcacctcgtc ggccgataca cccggggcca cctcgataag
     8221 ctccaggccc gtctgtgtca catcgatcac ggccagatcc gagatgatcc ggttcaccac
     8281 gcgcagcccc gtgaggggca agctgcacga gcggcgaacc ttggattgtc cgtccttcga
     8341 ggaatgctcc atcaccacga cgatacgccg cgctcccgag accagatcca tagccccgcc
     8401 catgcccttc accattttcc ccgggatcat ccagttcgcg atgtctccgt cctggctgac
     8461 ctccatcgcc ccgagaatcg ccagatcgat gtgtcccccc cggatcatgg cgaagctctc
     8521 ggcactgttg aatagagccg cgccgggcaa catggtcacc gtctgtttgc ccgcgttgat
     8581 gaggtcggca tcttcctcgg tctcgtatgg gaacggaccc acgccaagca atccattctc
     8641 gctttgcagg acgactgaca cacccggcgg tatgtagttg gccaccaggg ttggaatgcc
     8701 aatgccgaga ttgacgtatt gaccatcgcg cagctcctgc gccgcccgca tggccatctg
     8761 ttcccgggtc aacatgtcat gccacctctt cagccgcaga ctttcgggcg cgtggtcctg
     8821 cgctcgatgc gcttctcgtg ctgaccgacc acgatgtgat gcacgtagat ccctggggtg
     8881 tggatgcgat ccggggccag atctccgacc tcgacgagct catcgacttc cgccacggtg
     8941 atctggcctg ccatcgcgca caaaggattg aaattctggg cagtcaaacg gtattgcagg
     9001 ttgccggagc ggtcggcccg ccacgccttg acgatggccc aatcggtgcg gatcccgcgc
     9061 tcgagcacgt atttgcggcc atcgaattcg cgaacttcct tgtcttttgc caccagtgtg
     9121 ccgacgcccg ttggcgtgaa gaaggccgga atccctgccc cgccggcgcg caagcgctcg
     9181 gccagcgtgc cctgcggcac cagctccacc tcgagctgtc cggagatgaa ctgccgctcg
     9241 aattccttgt tctcaccgac gtagctggag atcatcttgc ggatctggcg attcgccagc
     9301 agcaagccaa gcccgaagtc atccacgccg cagttattcg aggctacggt cagatcgcgt
     9361 acgcccagct cgcgcagcgc ctggatgcac agctcgggaa ttccgcacag gccgaatccg
     9421 cccgccgcga tggtcgctcc atcccgaatc cccttgagcg cctcggctgc gctgcttcgc
     9481 accttgttca tggactttcg gctcccggag ccgcggatcc ggctcggtga ctcgatactt
     9541 taccgtcagc gaaaagcgcg gcgccagtcc accttaccgt gagctgtgcc catgacctga
     9601 cttcgatcgt atgatgcgcg ctggtcttcg ggagagcagc gccatgtcga ggtctcgtcg
     9661 cgaatttctg atgagctccg ccgccgcctg tctcggtcca gccgttctcg ccggtgcaca
     9721 gccagccaca cctgaaccgg ctgcggcaaa ggcaggcgag cccccgccgg gaaccccgcc
     9781 agccttcgga acggcgcctg cggtgggtcc agcggtaagc cccgcgacat tcgccgaagc
     9841 cgagaagctc gtacagatcg agctgaatac gcgtgaacgg gaggaggctg ccgcgaattg
     9901 gcgcaatagc atggctgcgc tgtacgagcg gcggacaggc ccgcgtcggg tagcgctcga
     9961 tgcgagtgtg gcgccttatt cgcagtggaa cccggtgctt ccgggtagcg ctggtatcgg
    10021 gcagcgggac cggttcgtcc gcagcgatct ggatccggga gcggtgccga agtccgaggc
    10081 cgcgattgcc ttcgctcccc tgtggaagct gtcgcgctgg atagagtcgc gccgactgac
    10141 ctccgaacat ctgacgcaga tctatttgaa aaggatcgat cggttcgatc cggggttgcg
    10201 gtgtgtgatc accctgacgc gtgagcttgc gctcgcgcag gcaaagcagg ctgaccagga
    10261 gatcgctgcc ggcacctacc gcgggccgct gcacgggatc ccgtggggcg cgaaagatct
    10321 gctggacacg gcaggcatcg ctacgacgta cggcgctgag ccgtttcgga accgggtgcc
    10381 ggagagcgac gccgcggtcg tgaagcgcct acgcgaggcc ggcgcagtgc tgatcgccaa
    10441 gctcagcatg ggagcgctgg cgctgaatga cgtgtggttc ggcggccaga cgatgaatcc
    10501 gtggttgccg caggaaggat ccggcggatc gagcgcaggc cctggagcag ccacggcggc
    10561 cgggctggtc gggtttgcca tcggcagcga gaccgagggc agcatcgtgg gtccgaccat
    10621 gcgctgtggg ctcacaggac tgaggcccac cttcggtcga gtaccacgca gtggtgccat
    10681 gacgctgtgc tggtccctgg acaagctcgg accgatgaca aggagcgtcg aggacacaat
    10741 gctcgtgctg caggcgatct cgggacgcga tgcggaagat cttgcgagtg ttccggcaca
    10801 cctggacttc gacaccaaag agagtcctgc cggcctgcga gtcggctatt tcccgaaatg
    10861 gatgaatgag gagccggcaa ccgaggtcga tcgcgccatg gtgcggcacc tcgaagagct
    10921 cggactgcac ccggtcgagg tgtcgattcc cgactggccc tacgatgggc tcaatccgat
    10981 cctgttcggc gagtccgcgg ccgccttcga agacatcacg ctctcgcgcc agattgattc
    11041 gcttcgcatg cagacaccgg atgcctggcc gaacactttc cggcagtcgc gtttcgtttc
    11101 cgccgtggat tttgtgcagg ccgatcgatt ccggcgacgg gtcgcgcagg aaatgcagaa
    11161 gctgcttgcg caggtcgatc tgctcctggt gccttccctg cgggatgaga tgctgaccat
    11221 tggtaatttc accggccatc cctgcctgac gctgcgtgca gggttcgtgc acgttaccga
    11281 ggcgcgcagc gattgggcac ccgacccgca gcatccgctg cccaagttca aacagccgag
    11341 gcgggtaccg catggcatca ccctggtcgg ccgcctgttc gatgagggca cgatcgctcg
    11401 cgtggggctt gctctggagc gcaagttgaa tgtggccggt gagccgccgc caggatttca
    11461 gtgaaagcgg cgtcccggtc cggcgttctc gccgtcacac ttcaagccgg tcggctgggc
    11521 gagccaccag agcatacagc gccggcatca ggaaaacgct gatcagcagg cgcgccagca
    11581 gaccactcac gatgaccagg gcaaacggac gctgtgagtc ggtgccgacg cctgtcgcga
    11641 gcgccgcggg caccaggcct agcgccgcga ccagtgcggt catcatgatt ggccggagcc
    11701 ggaggattgc cccgtctcga atcgcacgct cgaggggtat gcccgcaagc cgcaactcat
    11761 tgacgtaaga gatgtacacg actgcggtga gcaccgacac gccgaacagg gcaagaaagc
    11821 cgatcccgga agagacggaa aacggcgtgc ccgtgatcca cagagcgacg atcccgccga
    11881 ccggggcgga aagcagtaca ccgacgacag tgatgaaggg gaacttgaaa ttattgtaga
    11941 gcgcgaacag gagcagaaag atcaaaaaca gcgttagcgg caggatgaca ttgagctgtg
    12001 cacgcgaggc ggtgtattca gtgtattcgc ctccccaatc caaccggtac ccttggggca
    12061 tactcacgtg cgagctcacc tgttgcatgg catcgccgac cgcgccggcc aggtcacgcc
    12121 cctcgacgga gaactgcaca ccaatatagc gcgagttgtt ctcgcggtag atgaatgcgg
    12181 cgccgttggc gacccggata tcggcgaact ccctgagagg tatctgctgt ccggagggcg
    12241 tagccaccag gatgttgccg atttcctgcg catcgtcccg atactgacga tccaggcgca
    12301 ccaccagatc gaactccttt tcctgctgca ccacctgcgt cgccacatcg ccgccgatcg
    12361 ctgtctggat caaaccattg atgtctgaga cgttgaggcc gtagcgggca atgcgctggc
    12421 ggtcgatcgt gatcgtcaag ctcggctggc cgagttcctg tacgagcgtc acgtcgcgta
    12481 tcccgcgcac ccgctgcagc acctgtttga tggccttgcc tttctgctcc agcgtgttga
    12541 ggtcggaacc gaaaaccttg actgccaggg cgctcttcag gcccgtctcg gcctcatcca
    12601 cggcgtcctc cgccggctgt gtatagttga aagtaatgcc tggaaaagca gccagcttgt
    12661 cgttgaccac ttcgatcagg ccggccttgt tgcggtaagg ccccttccac tgtgcatagg
    12721 gcttcagacc gacgtagaac tccacattga aaaaccctgt agggtcggta ccgtcatcag
    12781 ggcgtccaag ctcggaagtc accgttgtga cctcaggaaa gcggtgcagc acttggcgca
    12841 cttgcggtgc gatcttggcg gcttcgtcga aggagatggt gtacggcatc gtcgcccgca
    12901 cccacagtgc cccttcgtcg agcttgggca tgaattcggc accgatgctc ggaacaagca
    12961 gcaaagagag tccgagcagg atcgccgagg cgatgatcgt agcccagggc cggtcgaggc
    13021 agtaatcgag tccccggatg tatattgact gaatggcctg gaaggcggga ttgcgcttct
    13081 cctgcgcgcc atgtctcagg aaccacgagc acaggaccgg caacagagtc agcgtgacga
    13141 tgagcgagcc gaccaaggca aaaatcatcg tgtcggccat cggtttgaaa agcgtgccgg
    13201 agggccctga gagcacgtag atgggcagga atccggcgac gattaccgcg acggcataga
    13261 agagtggccg atccacctcc gcggccgcgt ccctgatgat cgccatgacg tcgagcgtca
    13321 cccccgcagg acgtgaggcg acctggcggt agatgttctc caccatgaat atcgccccgt
    13381 ctaccaggat tccgaagtcg actgcgccga tcgaaaggag gttggccgag gcgttctgca
    13441 ggtgcaggca gaagaaggca aagagcagcg ccagtggaat ggtcgcggct acgatgagtc
    13501 ccgcccgaag gttgtacaga aaaaatatca ggacgacgag taccaggagc atgccgcgca
    13561 gcaggttctg ctccacgacg tcggtggtga gtgcgatgag attgctcgca tcatagaagg
    13621 gcactacctt gacatccttg ggaaggatct cctgattgag ctcgagcgtt ttggcctcga
    13681 cgcccctgag cacatcctgc atcttctcgc cggttcgcag caggatgacg ccctctacgg
    13741 cgtcgtcctg cttctcaaag ccgaactcac ccagccgcgg ggcgatgccg atgaccaccc
    13801 tgcccacgtc cttcacgaga accggatttc cgttatgcac agcaaccaca acgttgccga
    13861 tatcctccag cgtcttgatg cggccaaggc cccggacgta gtagaactgg ccgccctccg
    13921 agtagaagcc accccccgca ttgtcgttat tggcggtcag cgagctctcc acctgctggg
    13981 gcgaaagacc gacggaggcg atctttaccg gatcgagcag cacctgatac tgcatggttc
    14041 cgccgccgaa gccggagtca tccgccacac cggccacaga acggtactgg ggctcgacca
    14101 cccagtcctc gaaggtcttc agctccatcg gggagcgatc tcggctctgc agcacatatc
    14161 gatagatcaa gcccgagggt gcggagagcg gggcaagtcc aggagcgacg ccgcttggaa
    14221 ggctcagctc gttcagcctt tcgaacacga gctggcgcgc gaaataattg tcagtgccgt
    14281 tacgaaaagt caggatgaca tccgacagac catataggga gatggagcgg atcgtcgtga
    14341 gctgcgggac gccgttcatc ccgcgctcgg cagggacagt gataagccgc tccacctcct
    14401 cggcagcatg cccgggccat tgcgtaatga tttcgaccat gggaggcgac aggtctggat
    14461 aggcgtccat cggaagcctg tcgaatgccc accagcctgc gccaatcaga atgcaggtca
    14521 tgagaacgac cagaaagcgc tgtcgcagcg acgatgcgac gatccggttc atgaccgagc
    14581 gcgggcgcgc agccgaaccg ctggggaact cgctcccgct cattggcttt gcatgaactg
    14641 aagaaacagg cccccggaaa ccacgatctg ctctcccgca cgcaatcccg ccgaaacgtc
    14701 gtatttgtcg gccgcgcggt atcccagcgt cacgtgccgc ctggcaaagc tgccgtctga
    14761 ctgggcaaca tagacgaacg gcaagttctc atcgtcgtgt agcaccgccg ccgcgggaat
    14821 cagcaaccca gtgctttcct ggtgatcgtg aatcagcact cgcacgtaca tctgcttctt
    14881 gagaaagcgc gcgcggttgt cggccacgac ccgcacctgc acggcgcgcg tatcggggtc
    14941 gacgagcgcc gagatgttgt cgaccctgcc ggagaaattg ttggcgccga cgcccgtcac
    15001 gatgtcggcc gcatcgccca cgctgacgga tgccaggtcg gcgccgaaaa gctgggccat
    15061 gacccacacg cgcgaaagat ccgccacggt gaagcaggcc gtcgtacccg cctgcagcag
    15121 ctctcccggg gagatcagcc gctcgacgag cgttccggag atcggcgagc ggatcaacgc
    15181 accgggctcc gacaccggtc ggccttcctg gatctcggtg atggtacgcg tcgggacgtg
    15241 tagcgagacg agctgtcgaa gtgcggcgtc ccggtcggcc tcggcgctgg tcgcatcgct
    15301 ctgcgcctgg tcctcttcac gctgcgacac gccattgtgc tgaatgaggt ccttgtccag
    15361 gtccgccagc cgacgcgccg tgcgagccgt ggcgatcgcc ttgcgatagg tgctcatagc
    15421 cgtcgcaaaa tccggcgatt cgacgaccgc gaggggctca ccctcagtga cttccgcgcc
    15481 aagggggacg agaaggcggc tcacgggtcc cgagaacggg gccagcacgc tggtggactg
    15541 atcgttgtcg aaatccacga caccgctcgc gtcgaccgtc cgctggtatc tcaggggcgt
    15601 gaccttgtag aggttgacgt gtgtgcgctg ctcctgggtc aagctgacac cgtcggcgcc
    15661 cggcgaggtg tccggcttgg ggtcggcgct acgtgagcag ccgacgagca gagaagcggc
    15721 cagagccagc agaagtacgc gtgcatgcac agcccggcac ggggctttca cagcgggccc
    15781 cccacgcatc ggatcatctg ccattgctgt tagaggtcag atccgcgcgg ttccaccagc
    15841 caccccccag agcctggaac agggcggccg tgtcggcgta gcggctcgcc tgtgcctgaa
    15901 ccagactgat gcgagcctgc tggtaggttt gctgggcgtt cagcagcgac aggtaattcg
    15961 catagccggc tttccactgt cgctgcgcga ggtcgaggga gaccttggct gcatcggtcg
    16021 atgccgcggc agagttcagg gcgtcaccat cctgctcgag tgctgtcagt gtgtcggcga
    16081 cgttctgaaa agcggtgaga acggtgctgc ggtactgttc tgccgcctgc tggtacgccg
    16141 ccttcgcagc ccgctcctga tgcagcaacg tgccaccctg gaagatcggg gccgtcgcgg
    16201 tagcgcccag cgaccagaaa tccgtcgcgg tgctgaggag ctggctcatg ctcagcgcag
    16261 agctgccggc gttgcccgtg atcagcaagt tggggagccg attggcgatt gcgataccga
    16321 tcttggcgct ggcgtcgtgc aggttagcct ccgcctgcag cacatcgggc cgctgccgca
    16381 ccagcgtgga gggcacgctg accggcagct cctcgggcag tttcaggctg gccagctcga
    16441 agtctcctgc cgagggctgg ctgggaaact ttccgaccag taccgcaagc tgatcgcgct
    16501 gttgtgccag ctgcttgacg agcggcggga gcgtcgcacg gatctgtgca agctgtgctt
    16561 cctgcgtcgc cagatccacg ccgctcgcat agcccttctc gagctgatag cgcaggatct
    16621 gcaccatgtt ggtattgagg tcgatcagct cccgggtggc gtcgatttgc agctgcgtcc
    16681 cggcgagctg aatcgccgtc accaccacgt tggcggtcag cgtggtatac gtcgcgagca
    16741 tctgatagcg aacgccctgt tcctgtgcct gcagcgactc gacggtgcgc cgattgaggc
    16801 cgaagacatc gggtacgtag gtcaccgtca cctgcggtgt gaacaggttg taaataaaag
    16861 tattggagtt gggcaccggg gcaagctgta cggactggcc ttgccgcgtg gccgagaagc
    16921 tggcgctgac tgccggatag tagacgccgc gttgcgccag caggttctcg tgtgccacgg
    16981 acagcgcggc ctgcgcagct ttcaggtcag ggttgttcgc tagggcatga tcgatcagat
    17041 cgttgagggc cttggaatga aacagggtcc accagtcggc cgagatgtct gcaccgggcg
    17101 caaactgctg cggctcaccg cccgtgacgt gctcggttgc ggcaatgctc tcgagggggt
    17161 gctccgtata agtgctgaca tcgggggcgg cgggccgctt gaagttcggt cccacggcac
    17221 agccggcgat gagcaacgga gccaggaccg ccaagagcag cggcggtcgg cgctgggaaa
    17281 tctggctcat gcagtcagta gggaatcgtg gcgcgctctt ccttcttggc tttcggtggc
    17341 ctttcacgcg cagccctgaa aaccgtcatt gtccgcgaaa gcccgggcgg ccgccagagg
    17401 gtgctcaccc acggacgaga ggccatcgca agcggttcgc cgtgaacgcg tgacaggctg
    17461 ccatcggctc gacaggcagc tcgcaaccgg gttcgtgatg ggaccgcaca aaccgccgaa
    17521 aattccgcca ggggcacgcg ctgcaagcgc gaccgtggcc ggctgtagca agatcgcccg
    17581 tccgcggcgc ctgccgggcg gcaatggtaa gctcgggaaa aactcccgcg cgtccattcg
    17641 gacaggttgg cgcgcggctc gacaggttgg tacgcgcggc gacaacggct cgggggctct
    17701 acgatcacag gtccaggcgg cggcaggctt ttcggcacgg ggcaacctgc gcgctcgcgc
    17761 cggcccctga cctgggcggc ttcgtgcgtc ctcggtggcc tgctgatgag cggctgcgcc
    17821 cattacgaag ccaagcctct cgcccccacg cgcagcgcac gggcgttcga ggaacgatcg
    17881 cttgcttccg actcggtatg gcaatcggtc gccccgctgc tcggggacgc cggccgcacc
    17941 tggcccccag ctcaatggga ccgggcgcag cttctggcgg tcgcgctcgc cgagaatccg
    18001 gagctggccg tgatgcgcgc cgccacccag gtcgctcttg cacagcaggt ccgcgcgcat
    18061 gaatggccca accccgagct gacattgcag tcggagtact cgcgcgggga gcggtatacc
    18121 tggttgtatg gtgtcggctt cgatttcctg ctgctcgcgc cccgccagcg ccgtatcgat
    18181 ctggagatcg cgcatcttgc gacactcagc agtgcatggc agctgacgga gaagacctgg
    18241 caagtgcgac gcgccctgat cgaggctctg tccgactggc agcaggcccg aaatgaggcc
    18301 cctctgctca ggcagctcat ggactcgcaa cgtcatctgg tcgagctgca ggagcgacgc
    18361 gttcatgccg gagaggatcc accgagcgag ctgatcgcgg tgcgagtcag tctgatcgag
    18421 accgagcagc aactagctca gtctcgagct gccgcaagca gcgctcaatc ggccgccgcg
    18481 gcggccttgg gcatgccgcc ggctgcactg gatggcgttg ccattgaatg gcaggactgg
    18541 ggtcggcccc cggatctgga ttcgcaggag atgccggcgt tgcgcgaggc ggcactgctt
    18601 gcccgggccg atctcgcctc tgcgatcaat gattacgcgg attccgaact gcgactgcag
    18661 ctcgcgatcg cacgtcagta tcccgaattt cacctcgatc ccggctacta ctgggatcac
    18721 ggcgtgggaa agctgccgtt gaatctcggt ctggtactgc cggtcttcaa ccgcaaccgg
    18781 ggcgagatag ccgaggcccg ggcaggtcgg gagctggctg gccaacgcat gctggcggtt
    18841 caggcggaca tctacggcag catcgaggct gcactgcgag aagatgcgct ggctgaggag
    18901 agcgtggcgg ccatcgatcg gcagcagacc cttgcggacc ggcagagcca gcaggcgcag
    18961 ttgggcctgc aggccggcgc cgtcagtgcg agcgagcgcg ttgcagcgca ggtggtggca
    19021 ttgcaagcgg cgctcgaggg cgtgcgcgcg cgcgcccggc gtcaagcggc gcgcaatgca
    19081 ctggaggatg cactgcatgc tccgctgagt ggacctgaga aggcgctcgg gggtgcattg
    19141 ctgaggcggc ccgggctcgc tgaatccaac ttgagcaagg agtccaggcg gtgaggcgcg
    19201 gacttgcggg cagcgctctg gcggccctgt tggcgtgcgg cgtcatgacc acgcacgctc
    19261 aggatacaaa tgaccgaccg caagccgaag acacatcgaa agccgccgca gagagcggca
    19321 gcgcggttcc gaccctgacc ccgcaacagc gggatcgcgc agatatcgcc gttgcccatc
    19381 cacgagctgc gaatggcgct cagcactgga gcgcattggg ccaggtgctg gatgcctccg
    19441 cgatcgtcgc cgacttcggt cagctcgatg cagctgcggc gagcgagcgg gccgccgatg
    19501 ccgagctgca gcgcttgcag ggactgtacc gggcggatgc ggcctcgtcg cttaaaatgg
    19561 ttcagtccgc acaggcggaa caggtgcgtg cccggaccca gcatgaagtt gccctcgccg
    19621 cgtgcgttgc gcggtgggca ccgatcgccc gcctacccga ggagcagcgg caccagatga
    19681 tccaacatgc cgcgagcgga caacatctgc tggtgcgcgc aagccttctc ggccggcaga
    19741 gtatcggcca gctccccgag tcggccatgc tgcaagtgga cgggatgcag gttcctgccc
    19801 gggtgctggg aatcaccgcc cagggctcgg cggatctgca gagtgccggc attctgttgc
    19861 tcgtgagcgc cgcacccgcc ggactcgggc ctggcgcgcg gattcccgtc acgctgatga
    19921 tggcggcgcg cagcggcgtt gtcgtgcctg acgcggcgct gatttacggc acccacgggg
    19981 cgagggtctt caagcagctg atgggcggaa gcgggaagga aacgtggcac tacgtcgaag
    20041 tgccaatcga tctgctggag cggcaggcag atggctggct cgtgcggggc gtcagcagcg
    20101 ccgatctgat cgtggtgcgc ggtgccgggg cactgtggtc gctggaggct gccaacctcg
    20161 ccgccggtcc tcaagaagac gatgatgatt gacggaccgc gctgatgctc gcggccatcg
    20221 tccggatctc gctcgctaat ccgcgaacgc tggccacgtt ggccgtgctg atgtcggtgc
    20281 tgggcgcggc ctccctgacc cgcgcgcact tcgatgtttt ccccgacttt gcgccgccgc
    20341 atgtcttcat ccagaccgag gcccctggct tcgacgcaag ccaggtggaa ggactggtca
    20401 cccggcctct ggagagcgtc ctgggcggcg gagccgatgt gaaaacggtg cgctcgacct
    20461 cgagccaggg actgtcggtg attcaggtcg tgttcgccag ccgcggcgac ccttacaggc
    20521 aacgccaggt catcgccgag cggctcggcg aggcggtacc gctgctgccg caaggcgtgc
    20581 acgctccgca gctctcgccg ctgagctcat cggtggagta tctgcttcat ttcggcttca
    20641 ccagcccgac actctcgccg atgcagctgc gcgacatcgt cgagtggtcc gtgaagccgc
    20701 agatccttgc cgttccgggt gttgctcaag tgcagatatt cggcggagaa gtgcgcgagc
    20761 ggcaggtctt catcgaccct caacgactcg cggctctcgg cgtgagcttc gaagacgtcg
    20821 tggccgcggt cacgcgcaac acacagctca gcggcggtgg attcgtcgag agcgcggcgc
    20881 agcgcatcgt gctgcggccc cagagtccgg gcatcgcgcc cgaagagctg gcccagtctc
    20941 tcgtgctgac gcgtgacgga gtgcccatcc gtctcgccga tattgcaagt attcaggacg
    21001 gacccgctcc gcgcttcggc gctgccctga tcgacggtaa gcctggactg ctggtcgaga
    21061 ccgcaacgca gttcggggcc aacacgctcg aggtctcgcg cctgctcgag cagaagctga
    21121 atgagatcgc cccggtgctc cttgcgcagg gggtcgagta tcgtccggcg ctgcttcgtc
    21181 cggccagctt cattgaagat gccgtgcgcg agttgcgcga ttccatcctg attggcgcga
    21241 tcctcgtcgt c
//
LOCUS       DASRHN010000335         8889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1276744, whole genome shotgun sequence.
ACCESSION   DASRHN010000335 DASRHN010000000
VERSION     DASRHN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276744"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFB37_13430"
     CDS             <1..873
                     /locus_tag="VFB37_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23499.1"
                     /translation="SRLEALMKIYPQIISTVAIHKDAPAVPQERMVYMDVRVVEMLKS
                     TVRQLGVNWVSGANESSAGPSLNLNLSGSKNMLKVPNGGVFGIATNLSSVLDYTERAG
                     ESWTLAEPSVSCKSGGSAKFQVGGEIPISVAGGLGTVNVVYKEYGVILEFKPVADDKG
                     NISSSIVAEVSEPDSKFSNVGGNGLVAFTKTRTETDVSLKENQTLVISGLLSDVGNRA
                     ATGIPGAKDIPVLGNLFKSRQFQNDRTELVVLVTPHSMAVGNELNAASIRRADEINTQ
                     LAPTIKYINSRLVE"
     gene            878..2611
                     /locus_tag="VFB37_13435"
     CDS             878..2611
                     /locus_tag="VFB37_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZO23500.1"
                     /translation="MWTIHLQESGGRQSDPRIATGKVKIGRDADCDVKLSGWRIGNKH
                     AELFVSNEQGYVRDLGASSGTLVNGKAVTTYGPLTAGDKIQIGPHFLTATWLAELAQT
                     PGPAAAPVSQSLQTVPALVSASAVSAPAMPSSAASGEAETPAKPAFQDETFTWRRMLH
                     DRLLEAFDVRRVDVHRMADGELRARAEQLIAELIERMPDIPKQIDRTTLLAAVRDEAI
                     GLGLLEPLLADASVTEIMVNRSDEVFVERAGVLERYPLAFTSDRAVTGIIERIVAPIG
                     RRIDESSPMVDARLKDGSRVNAVIPPLALKGPSITIRKFSKRKLKADDLQRFGSASAE
                     MIEFMKVTVEHRKNVVISGGTGSGKTTLLNILSNFIPRGERIVTIEDAAELQLHHPNL
                     VSLESRPANIEGKGQIAIRDLVRNSLRMRPDRIVVGECRGGEALDMLQAMNTGHDGSL
                     TTAHANNPRDMLSRLETMVLMAGLDLPVSAIREQIASAIDIVVQQTRFACGTRKITHI
                     CEVTGVESGRIQLQDIFEFRQTGVDQAGKTQGYFTGCGFVPEFYESLRRIGIPLDLGI
                     FERRETAGVAA"
     gene            2608..3456
                     /locus_tag="VFB37_13440"
     CDS             2608..3456
                     /locus_tag="VFB37_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZO23501.1"
                     /translation="MNANLTPVLTGLMFVAAAIALVKVGTALYAGYRQRFTEAARSRL
                     EEAFVFVDPGKLFGLTMLCTAVIPLLLWFLNGGLVLPVLAAVVINVAPRFYLRWKNQR
                     RRLQIVTQLPDVLRMLGSSLRAGTSLQIALDIAIRETPAPLSQELGVVVREQRLGLAL
                     EDALDSMAARLKLEEVELVVAAMTIAREVGGNLAETLDQLATTLRAKATMEGKIRSLT
                     SQGKLQGWIVGSLPLFLMLVLSYMQHDAMKPLFHTLIGWLVLVLIGILEMVGFAMIRK
                     IVTIDV"
     gene            3502..4371
                     /locus_tag="VFB37_13445"
     CDS             3502..4371
                     /locus_tag="VFB37_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZO23502.1"
                     /translation="MTDLLFPALVLSAVFLAVFSLRRIVKEVPKDDRQYMDPPPRFYR
                     MTWPLVRLVSHYCGWALSQGMAIRIQEALRRAGQDYTLSPVQFFAGKIVAALLFGAGL
                     AWIATKMKLTALAGFMPGAALGFLLPERWLADMTKTRNLRILKSLPFFLDIVTMAVEA
                     GMNLSSALQKSVEKTPPGPLLVEINRVLREIRAGKPRADGLRDMAARLNFQAMNSLVS
                     ALVQGELMGSSIGPILRAQAEQRRTERFQRAEKLALEAPVKMLGPLILCIFPCTFIVI
                     AFPIVTQLMKSGL"
     gene            4368..4727
                     /locus_tag="VFB37_13450"
     CDS             4368..4727
                     /locus_tag="VFB37_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZO23503.1"
                     /translation="MKQQYQHCATAAGALQVRVAKSFLSRALGLLVGAPLQPGEGLLI
                     SPCSSIHTLGMRYPIDVVFLDRDGCVMRVYRSVQAGRLRFGLGARGVLELCAGAAATH
                     GLTPGVRLSELARSLAG"
     gene            complement(4733..5650)
                     /locus_tag="VFB37_13455"
     CDS             complement(4733..5650)
                     /locus_tag="VFB37_13455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZO23504.1"
                     /translation="MKSSSESRTGPLAEQSRPNDARSGSPGEQLTRLGSYLLLGVISQ
                     GSMATVYRSSDPGTGRAVAIKAVRRDPAPQNAGENLAARLRVEAEVASRLSHPGIVAV
                     REYGEDALHAFIVMEYVDGVSLRQCFERRMSFGASRSISLVSQVLGALQYAHERGVWH
                     RDIKPANILVREDDLVKVTDFGIAQLAAPMAQQMDVILGSPGYIAPESYLGEKFDERA
                     DVFATGAVLYELLAGVPAFAGTADQIMFKVCNEAPLGPSIVAGMSSLQPYDELVLKAL
                     ARRPEDRFASAAQFRQALLGVAQNRRSGC"
     gene            5778..7586
                     /locus_tag="VFB37_13460"
     CDS             5778..7586
                     /locus_tag="VFB37_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZO23505.1"
                     /translation="MSPGTDTIIQGAIDSRNVPAMFAALPLLRELAGELLAEITQEIE
                     WFSLPGGSTLYKAGQSSDGLYVVVNGGLGMYVARSGGGARLAAQISAGQLIGEAEVIS
                     GNRRASTVVALRDTELARLPTALIEHLVAKNPGAMRAIAKILVQRLESEPGDRQQKAR
                     PKTFAVIPHGPDVDAEAFGRQLLHCLKGLGRAELVLHSTGGEQTSHWFHRLERANDYV
                     AYVTDFRPSNWSKLCLRQADSLLLLARADSTAHAWPALLAREEQPDKLQRFELVLQRS
                     GAKRTPTRDWMNLYPCRHHHVSSQADVARLARTLTGRAVGLVLSGGGARGFAHIGVMR
                     ALREARIPVDGIGATSIGAIIGAGWAAGWDYAEMVQRIRRSFVDTNPTRDYTLPLVSL
                     LSGRKVCRLLRREFGETNIEDLPLPFYCVSTNLTSGQAAVHRQGPVWLWSRASVAIPG
                     VLPPVIADRQVYVDGATISNLPVDIMRELLDGPVIAVDAGADRAFATDLELTEMPPLW
                     GLPTWRQLRRSRINIMQILWRAGMVNSAATTIGQRELADLLLKPPTDSINLLDWKAFE
                     RAIEIGYRHASEALEKRDWLERRLAPERSGVHPTGQ"
     gene            complement(7580..8491)
                     /locus_tag="VFB37_13465"
     CDS             complement(7580..8491)
                     /locus_tag="VFB37_13465"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZO23506.1"
                     /translation="MAEKRFILKKLPDGPEIPVDGELCVGRSAESGLKLVEGRPSRNH
                     AVLSIVDGSVFVEDLGSTNGTFVNDKRIDSKVRLKSNDKLRFDVEQYLFRIEMAGSPA
                     DRTLLREGEPDLVVASGKARVPEGWLENPQAGNKTLYMSPEQLRQERKRLAALGAVDG
                     ALGRVEAPQLIVLGGTEPLRIELKPTESGKKEWSVGSEGEREILLKRVGVSALHAKIV
                     NEGNRWKVIDELSANGTFVNGRRCTMSFLSSTDRITFGSVECIFQLPKGAQSAAANSE
                     GRPWKKLAIGAIVLIVIAAAAWFFLAH"
     gene            complement(8660..>8889)
                     /locus_tag="VFB37_13470"
     CDS             complement(8660..>8889)
                     /locus_tag="VFB37_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23507.1"
                     /translation="ASVRKTVLRYAAQDKASDFSTAEQSVMCVESLSYALGDHDRHKA
                     ALDALYGAIKSASAFDPTQFAATARSVGAQF"
BASE COUNT         1549 a         2911 c         2844 g         1585 t
ORIGIN      
        1 agcaggctgg aggcgttgat gaagatctac ccgcaaatca tcagcaccgt ggccattcac
       61 aaggacgcgc ccgcggtacc gcaggagcgc atggtgtaca tggacgtgcg ggtcgtcgag
      121 atgctcaaga gcaccgtgcg ccagctcggc gtgaactggg tcagcggtgc caatgagtca
      181 tccgcagggc ccagcctcaa tctgaatctc tcgggaagca agaacatgct gaaggttccg
      241 aatggcggcg tgttcggaat cgcgaccaac ctgagctcgg tgctcgatta caccgagcgc
      301 gccggggaaa gctggacgct ggccgagccg agcgtgagct gcaagagcgg cggctcggcg
      361 aaattccagg tcggtggcga gatcccgatc tccgtggccg gcggccttgg cacggtgaac
      421 gtggtctaca aggaatacgg cgtcatcctc gagttcaagc ccgtagcgga cgacaagggc
      481 aacatcagct cctccatcgt cgccgaggtc agcgagcccg acagcaaatt ctccaacgtc
      541 ggcggcaacg gtctggtcgc tttcaccaag acccgcaccg agaccgacgt cagcctgaag
      601 gagaaccaga cgctggtgat ctccgggctt ctgagtgatg tgggcaaccg ggcggccacc
      661 ggaattccgg gcgccaagga catcccggtg ctcggcaatc ttttcaagtc caggcagttc
      721 cagaacgacc gcacggagct ggtcgtgctg gtgactccgc actcgatggc ggtcgggaac
      781 gagctcaatg cggcttcgat ccgccgtgcg gacgagatca acacgcagct ggcacccacg
      841 atcaagtaca tcaacagcag gctggtggag taaagacatg tggaccattc atctgcagga
      901 gtccggtgga cggcaatccg atccgcgcat cgcgaccggc aaggtcaaaa tcggccgcga
      961 tgccgactgc gacgtgaagc tttcgggctg gcgcatcggc aacaagcacg cggagctgtt
     1021 cgtttccaat gagcagggct acgtgcgtga tctcggcgcc tccagtggaa ctctcgtgaa
     1081 cggcaaggcg gtcacgacct acgggccctt gaccgcaggc gacaaaatcc agatcgggcc
     1141 gcatttcctc acggcgacct ggcttgcgga gctggcccag acgcccggtc ccgcggctgc
     1201 tccagtgtcg cagagcctgc aaaccgtccc ggcgctggtc tctgcgtcag cggtctctgc
     1261 gcccgcgatg ccctcctcgg cggcgtcggg cgaggccgag acgcccgcca aacctgcctt
     1321 ccaggacgag accttcacct ggcgccgcat gctgcatgat cgattgctcg aagccttcga
     1381 cgtgcgccgg gtcgatgtgc accggatggc ggatggcgag ctgcgcgccc gggcggagca
     1441 gctgatcgcc gagctcatcg agcgcatgcc ggatattccg aagcagatcg atcggacgac
     1501 cctgctcgcc gcggtgcgcg atgaagccat cgggctcggt ctcctcgagc cccttctcgc
     1561 cgatgcatcc gtgaccgaga tcatggtcaa ccgatcggat gaggtgttcg tcgagcgcgc
     1621 cggggtcctg gagcgctatc cgctcgcctt caccagcgac cgggcggtca ccggcatcat
     1681 cgagcgcatc gtggcaccca tcggccgccg catcgatgag agctcaccca tggtcgatgc
     1741 gcgcctcaag gacggctcgc gcgtgaatgc cgtgattccg ccgcttgcgc tcaaaggacc
     1801 cagcatcacc atccgcaagt tctccaagcg caagctcaaa gcggacgatc tccagcgctt
     1861 tggctcggcc agcgccgaaa tgatcgagtt catgaaggtg accgtcgagc accgcaagaa
     1921 cgtcgtgatc tcaggcggca cgggctccgg caagacgacc ttgctcaaca tcctctcgaa
     1981 cttcattccg cgcggcgagc gcatcgtgac catcgaggat gctgccgaat tgcagctcca
     2041 ccacccgaac ctggtatcgc tcgagtcgcg tccggccaac atcgaaggca agggccagat
     2101 cgccatccgg gatctggtgc gcaattcgct gcgcatgcgt cctgatcgca tcgtggtcgg
     2161 cgagtgccgc ggtggcgagg ccctcgacat gctgcaggcc atgaacaccg gccacgacgg
     2221 gtccctcacc accgcgcacg ccaacaatcc gcgcgacatg ctgtcccgtc tggagaccat
     2281 ggtgctgatg gcaggcctcg atctgcccgt cagcgccatc cgcgagcaga ttgcctcggc
     2341 catcgacatc gtcgtgcagc agacccgctt cgcctgcggc acgcgcaaga tcacccacat
     2401 ctgcgaagtg accggcgtcg agagcggtcg cattcagctt caggacatct tcgagtttcg
     2461 ccagacgggg gtggaccagg ccggcaagac gcagggctat ttcacgggct gcggcttcgt
     2521 acccgagttc tacgagtccc tgcgccgcat cggcataccc ctggacctcg gaatcttcga
     2581 gcgtcgtgag acggcgggag ttgcggcatg aatgcgaatc tcacgcccgt cttgaccggg
     2641 ttgatgttcg tcgccgccgc cattgcgctg gtgaaggtgg gtaccgcgct atatgccggg
     2701 taccggcaac gctttaccga ggccgctcgc agccgcctgg aggaagcgtt cgtcttcgtc
     2761 gatcccggca agctgttcgg cctcacgatg ctctgcacag cggtgatccc actcttgttg
     2821 tggtttctga acgggggcct cgtgttgccc gtgctggcag cggtggtcat caacgtggcc
     2881 ccgcgcttct acctgcgctg gaagaatcag cggcgcaggc tgcagatcgt gactcagctg
     2941 cccgatgtgt tgcgcatgct ggggagctcg ctgcgcgcag gcaccagcct ccagattgct
     3001 ctggatatcg cgatccggga aaccccggcg cccctgtcgc aggagctcgg agtggtggtg
     3061 cgtgagcagc gcctgggact tgcgctcgag gacgcgctgg actcgatggc cgcccggctg
     3121 aagctcgaag aggtcgagct ggtggtggct gcgatgacca tcgcgcgcga ggtcgggggc
     3181 aatctggccg agacgctgga ccagctcgcg acgacactcc gcgccaaagc cacgatggaa
     3241 ggcaagatcc gctcactcac ctcgcagggc aagttgcagg gctggatcgt gggctcgctg
     3301 ccgctctttc tcatgctcgt gctgtcctac atgcaacatg acgccatgaa gcccctgttt
     3361 catacgctga tcggctggct ggtgctcgtg ttgatcggca tcctcgagat ggtcggcttc
     3421 gcgatgatcc gcaagatcgt caccatcgat gtctgaactt atcaacggat gcgctttgcg
     3481 cgcccggcac tggagaacga cgtgacagac ctattgtttc ccgccctcgt cctgagcgct
     3541 gtgtttctcg cagtgttctc acttcgcagg atcgtcaaag aagtgcccaa ggacgaccgg
     3601 cagtacatgg atcctccacc gcgcttctac cggatgacct ggcccttggt gcgcctggtc
     3661 agccactact gcggctgggc gttgagccag gggatggcca tccggattca ggaagccctg
     3721 cgccgcgcag gccaggacta caccctgagc ccggtgcagt tctttgccgg caagatcgtg
     3781 gcagcgctgc tgttcggagc cggcctggcc tggatcgcga ccaagatgaa gctgaccgct
     3841 ctcgccggct tcatgccggg cgcagcgctc ggctttctgc tccccgagcg ctggctcgcg
     3901 gacatgacca agactcgcaa cctgcggatc ctcaagagcc tgcccttctt cctcgacatc
     3961 gtgaccatgg cggtggaagc gggcatgaat ctctcctctg ccctgcagaa gtcggtggag
     4021 aaaactcccc ctgggccgct gctggtggag atcaaccggg tattacgtga gatccgcgcg
     4081 ggaaagccgc gagccgatgg gctgcgggac atggccgcgc ggctcaactt tcaggcgatg
     4141 aacagcctgg tgagcgcgct ggtgcaggga gagctaatgg gcagcagcat cgggccgatt
     4201 ttgcgcgccc aggccgagca gcggcgcacc gagcgctttc agcgcgccga gaagctggca
     4261 ctcgaggctc ctgtcaagat gctcgggccc ctgattctgt gtattttccc ctgcacgttc
     4321 atcgtgatcg cattcccgat cgtcacccag ctcatgaagt cgggcctatg aaacagcaat
     4381 accaacactg tgcgaccgcg gcgggtgcgc tacaggtcag ggttgcaaag agtttcctct
     4441 ctcgggcgtt ggggctgctc gtcggagcac cccttcagcc gggcgagggc ctgttgatct
     4501 caccctgctc cagcattcac acgctcggga tgcgctatcc gatcgacgtg gtctttctcg
     4561 atcgagacgg gtgtgtaatg cgcgtctacc gctcggtgca ggcagggcgc ctgcgctttg
     4621 gtctcggcgc tcgcggtgta ctcgagttgt gcgcgggagc tgcggccacg catggcctga
     4681 ctccgggcgt acggctctcc gagctggcgc gcagcctggc cggttgagtc ctctagcacc
     4741 cggaccggcg attctgcgcg acccccagca gcgcttggcg gaactgcgcc gcgctggcga
     4801 agcggtcctc tggccggcgc gcgagagcct tcagcaccag ctcgtcgtac ggctgcagcg
     4861 aagacatccc cgcgacaatt gacggcccca gcggggcctc attgcagacc ttgaacatga
     4921 tttgatctgc cgtgcccgca aaggccggca caccggccag caactcgtac aggaccgctc
     4981 cagtcgcaaa tacgtcggcg cgctcgtcga atttctcgcc aaggtaactc tcaggtgcaa
     5041 tgtaaccggg actgccgagg atgacgtcca tctgctgcgc catcggcgcg gccagttgcg
     5101 caatgccaaa gtccgtcact ttcacgaggt catcctcgcg gaccaggatg ttggccggct
     5161 tgatgtcccg gtgccacacg ccccgctcgt gcgcgtactg cagggccccg agaacctgtg
     5221 agacgaggct gatcgagcgg gacgcgccga agctcatcct tctctcgaag cactgtcgta
     5281 agctgacccc gtcgacgtac tccatcacga tgaaggcatg cagagcgtcc tcgccgtatt
     5341 cgcgtactgc gacgatcccc ggatgactca aacggctggc gacctccgct tcgacacgca
     5401 gccgggcggc tagattctca cccgcattct gcggggcagg atcgcggcgt acggctttga
     5461 tggccaccgc gcgaccagtc ccggggtcgg acgaccggta cacggtagcc atcgagccct
     5521 ggctgatgac gcccagcagc aggtaacttc ccagccgcgt gagctgttcg ccgggggatc
     5581 cggagcgcgc atcgttcggc cgggactgct cggcgagggg tccggtcctg ctttcggatg
     5641 aggacttcat gggcgggctg gaaaccgcct gacattagca cacgcgcgcc gcggcagctt
     5701 tcttgccttt acccggcagc cgctgtaccg tccgaccgcg cgtcggccgg cgcgacaact
     5761 cgtcatctcg cgtgcaaatg agcccgggaa ccgacaccat cattcagggc gcgatcgaca
     5821 gccgcaacgt tccggcgatg tttgcggcgc tccccctgct gcgcgagctc gccggagagc
     5881 tgcttgcaga gatcacccag gagatcgagt ggttctcgct gccgggcggc tcgacgctct
     5941 acaaggcagg ccagtcatcc gacgggctct atgtcgtcgt caacggcggt ctcggcatgt
     6001 acgtcgcgcg cagcggcggc ggcgcccggc tagcggcgca aatctccgcc ggccagctca
     6061 taggtgaagc cgaagtcatc tcgggaaacc ggcgcgcgtc cacggtcgtg gctctgcgcg
     6121 ataccgagct ggcccgcctg ccgacggcgc tcatcgagca tctggtggcg aagaaccccg
     6181 gcgccatgcg ggcgatcgcg aagatcctgg tccagcgtct cgagtcggaa ccgggcgacc
     6241 gccagcagaa ggcgcgtccg aagacgtttg ccgtgattcc tcatggcccg gatgtcgacg
     6301 cggaggcttt cggccgacag cttctgcatt gcctgaaagg actgggtcgt gcagagctcg
     6361 tcctgcacag cacggggggc gagcagacca gccactggtt tcatcggctc gagcgcgcca
     6421 atgactacgt cgcttacgtg acggacttcc ggccttcgaa ctggagcaag ctgtgtcttc
     6481 gacaggcgga ctccctcctt ctgctcgcgc gcgcggacag tacggcacac gcctggcccg
     6541 cactactggc tcgtgaggag caacccgaca agctgcagag attcgagctg gtcttgcaac
     6601 gctcgggcgc gaagcgcaca ccgacgcggg actggatgaa tctgtatccg tgtcggcatc
     6661 accacgtgag ctcacaggcg gacgtcgcgc ggctggcccg aacgctcacc ggccgtgcag
     6721 tgggcctggt cctctcgggt ggaggtgcgc gcggattcgc tcacattggc gtcatgcgcg
     6781 cgcttcgcga ggcacgcata ccagtcgatg gaatcggagc aacgagcatc ggcgccatca
     6841 tcggggccgg ctgggcagcc ggctgggact acgcggagat ggtgcaacgc atccgccgat
     6901 cgttcgtgga tacgaacccg acgcgtgact acacactccc tctggtgtcg ctcctcagcg
     6961 ggcgcaaagt ctgccggctg ttgcgccgtg agttcggcga gacgaacatc gaagacctgc
     7021 cattaccctt ctactgcgtc tcgaccaacc tcaccagcgg gcaggctgcg gttcatcggc
     7081 aggggcccgt ctggttgtgg tcgcgcgcct cggtcgccat tccgggagtt ctcccaccgg
     7141 tgattgcgga ccggcaggtc tacgtggatg gagccaccat cagtaacctt ccggtggaca
     7201 tcatgcgtga gctgctggat gggccggtca tcgcggtaga cgccggggcc gatcgcgcct
     7261 ttgcgaccga cctcgagctg acggaaatgc cgccgctgtg ggggctgccg acctggcgcc
     7321 agctacgacg ctcgaggatc aacatcatgc agatcctctg gcgggcaggg atggtcaaca
     7381 gtgccgcgac cacgattggt cagcgcgagc ttgcggacct gctgctcaag ccgcccaccg
     7441 acagtatcaa tctgctggac tggaaagcct tcgaacgggc catcgagatc ggataccgtc
     7501 acgcgagcga agctctggag aagcgtgact ggctcgagcg gcggctggcg cccgagaggt
     7561 ccggggtcca tccaaccggt cagtgagcca ggaagaacca cgccgccgct gcgatcacga
     7621 tcaacacgat cgcgcctatc gcaagcttct tccaggggcg gccttccgag ttggcggccg
     7681 cactctgggc ccccttggga agctggaaaa tgcattcgac ggagccgaac gtgatgcggt
     7741 cggtcgagct gagaaagctc atcgtgcagc gccggccgtt gacgaaggtg ccgttcgccg
     7801 acagctcatc gatgaccttc cagcggttac cctcgttcac gattttggca tgcagcgccg
     7861 aaacgccgac gcgcttcagc aggatctccc gctcgccctc gctgccgacg ctccattcct
     7921 tcttgcctga ctcggtgggc ttgagctcga tacgcagggg ctcggtgccg cccagcacga
     7981 tgagctgcgg cgcctcgaca cgcccgagcg ccccgtcgac cgcgcccagc gcagccaggc
     8041 gcttgcgctc ctgccgcagc tgctcgggtg acatgtacag ggtcttgttg ccggcctgcg
     8101 gattctccag ccagccctcc ggaacccgtg ccttgccgct cgcgacgacc agatccggct
     8161 ccccctcacg cagcagcgtc cgatcggccg gcgagcccgc catctcgatg cgaaacagat
     8221 actgctcgac gtcgaatcgc agcttgtcgt tggatttgag gcgcaccttg ctgtcgatgc
     8281 gcttgtcgtt gacgaaggtg ccgttcgtcg agcccaggtc ctccacgaat accgacccgt
     8341 cgacgatgct cagaaccgca tggttgcgcg agggccgtcc ctcgaccagc ttcagaccgc
     8401 tctcggcgct gcggcccacg caaagctcgc cgtcgaccgg gatttcgggt ccatcgggca
     8461 gctttttcag tatgaatcgt ttctcggcca tcctcggccc gagagtataa gcgatcccgg
     8521 ggacaggcaa ttccgccact gcgccggcgg cagacaacat attccggtgc aagtcggccg
     8581 ctgcgccggc cctacgcagc gcagacggtt cgccgcggcg tgcccgggct tacccggcgc
     8641 gaatagctcc gcgggagccc tagaactggg cgccgacgct gcgggcggtc gccgcaaact
     8701 gagtcggatc gaatgccgag gcgctcttga tggcgccgta gagggcgtcg agggcagcct
     8761 tgtgacggtc gtggtcgccg agcgcgtagc tgaggctctc tacgcacatc acagactgct
     8821 cggccgtgct gaaatcgctc gccttgtcct gcgcggcata gcgcagcacg gttttgcgta
     8881 cgctggcga
//
LOCUS       DASRHN010000336         8522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1936327, whole genome shotgun sequence.
ACCESSION   DASRHN010000336 DASRHN010000000
VERSION     DASRHN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936327"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3891)
                     /locus_tag="VFB37_13475"
     CDS             complement(<1..3891)
                     /locus_tag="VFB37_13475"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23508.1"
                     /translation="MSSGAAQQGIPARDTPEAILVRGARQNNLKNIDLDIPLKELVVV
                     TGVSGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVESISGIPPAIAIDQ
                     TNPVRTSRSTVGTMTELNDHLKLLFARTAALHCRCCGKPVRRDSSESIHADLLGRARE
                     AGDPRLLLSFPVPIPRNFTETEVRALLQKQGYTRFFGPALTAPAAPPAAAKKGRRKRA
                     VEAPLILEVVQDRFRAGGVERGRVLESLEAALRVGQGKVNVHVVDESDPPRALQTWRY
                     SSQLHCAECDLSYREPTPSLFSFNSPVGACESCRGFGRTIGIDYGLVIPDESKSLRGG
                     AIKPWQTKSYAECQDDLVKFARKRCIPLDTPWRELGEERRKWVIEGEGDWSKGVWYGA
                     KRFFAWLETKAYKMHVRVLLSRYRAYTPCAACGGARLKSEALLWRLGSQQLTDAALRG
                     SSAFRPQGAQWSDQRPPGLSLHDLMLLPLERVREFFAGLRLPQPLDEATDLLLGEIRG
                     RVGYLVDVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRD
                     MQRVIHVMKKLRDAGNSLVVVEHDPQIMLEADRILDMGPGAGERGGEIVFFGSPAEIR
                     QGAPSLTGEYLSGRRRVGAPAEGARSSAASALARPPAGIDPVSGQAASAQGEVVAEAR
                     SNRWLELEGASEHNLKGIDVRIPLKRLVCVTGVSGSGKSTLVKDVLYPALLKYRGKPT
                     ETPGEFTGLRGAELIDDVVMVDQNAIGRTTRSNPASYIGAFDSIRALFAAEPAAQERK
                     YTAGTFSFNSGNGRCPACSGNGFEHVEMQFLSDVYLRCPDCNGRRYRDEILDIKREGA
                     DGRRASIADVLEMTVSESLAFFADSRELALRLAPLADVGLEYVKLGQPVPTLSGGEAQ
                     RLKLAGHLAEAGSVLSSTTHRGKLFLFDEPTTGLHFEDVGKLLRAFRKLLAAGHSLLV
                     IEHNLDVIRAADWIIDLGPEGGDGGGQIVCTGAPAEVARHPGSFTGQALAAYERALSS
                     EPLPAQEIRAAGAAHAVAEPALLYRRADNVRGRNSIVIHNAREHNLKNVDVEIPRDKF
                     TVITGVSGSGKSTLAFDILFNEGQRRYLESLNAYARQFVQPAARPDVDAIFGIPPTVA
                     IEQRTSRGGPKSTVATLTEIYHFLRLLYVKLGTQYCPDCNVAIEPQGAGSIALRLLRD
                     FKGKRIALLAPLVLARKGYYTDLAKWAAKKGFKWLRVDGANTSTARWPRLSRFKEHTI
                     ELPVAEIDVDARSDTALHQALARALDFGKGLVHVL"
     gene            4567..4818
                     /locus_tag="VFB37_13480"
     CDS             4567..4818
                     /locus_tag="VFB37_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23509.1"
                     /translation="MNSRKMSSIALAAAAAVMFSTAFAGTASAADAKVKCEGVNSCKG
                     QSACKGANNSCKGQNSCKGQGFLEMTQAKCDAAKAKAGK"
     gene            4885..5721
                     /locus_tag="VFB37_13485"
     CDS             4885..5721
                     /locus_tag="VFB37_13485"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HZO23510.1"
                     /translation="MTLLRPSLGFGLGLRVDHYEAILADRPAIDWFEVLTENYLVPGG
                     KPLDYLMRIRERYRVAMHGVSMSIGSTAPLDRSYLTKLKALAARVEPEWISDHLCWTG
                     VGGHNMHDLLPLPYTEEALANVVERVRTVQDMLGRRILLENVSSYVSYHDSQLTEWDF
                     LREIAERADCLILLDVNNIYVSSVNHEFAPLEYLNAIPIERVQQFHLAGHENHGTYLV
                     DTHDHPVPDPVWELYCAAVQRFGNVSTMIERDANIPPLEELCAELDAARILAERTLAR
                     AA"
     gene            5675..6541
                     /locus_tag="VFB37_13490"
     CDS             5675..6541
                     /locus_tag="VFB37_13490"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HZO23511.1"
                     /translation="MRHASWQSAPWLAQRERRDGAARGSHRVSRLAHIQDAFQRFLIQ
                     RAPGIEAHVVGSERVSVATRLGIYGDGYRARLIEAMQSTFPVLATLLGEVDFANLAGQ
                     YVDTHVSSFSSIRYYGDELAAFLATDAEYSQVPLLAELAQWEWAMAGAFDAADVTPLD
                     VNAFAQLPPAQWAELRFDWSPSTQVLPLEWNVANLWKAVTEDQERPEPAVTPEPVNWL
                     IWRHELQIYFRSLPTDESAALAASRAGQNFGELCVLLCAHLEEDEASLRAASLLRGWV
                     QSGLIVGVARPP"
     gene            6551..7924
                     /locus_tag="VFB37_13495"
     CDS             6551..7924
                     /locus_tag="VFB37_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZO23512.1"
                     /translation="MSPQVCGNVLEMIGHTPLMAARHLDTGPCELFLKLENQNPGGSI
                     KDRVGLYLIEAAERDGRIRPGGTLIEATAGNTGLGLALVAAQKGYRLLLVIPDKMSQE
                     KIFHLKAMGAEVMLTRSDVSKGHPEYYRDLAERLARETPNAFYVNQFGNPANPRAHEE
                     TTAPEIWEQMGHKLDAVICGIGTGGTLTGLSRYLAREAPTVEIVLADPAGSVLAAFAR
                     TGQIPAEHGSWLVEGIGGDSIPPVADLSRVGSTYTIPDAEAFQTCRELLRREGVIAGT
                     STGTLLAAALRYCREQKSPKRVVTFVCDSGNKYLSKVYNDYWMLDQGFIERERFGDLR
                     DLIARRHWEHAAVTVNSTEPVLAALRRMKLYDVSQLPVMERGRIVGIVDEEDILLEVF
                     ANPDHFNEPVTKAMARGLVTVPIDAPVAQLMEIFKRGMVAIVVKDGEFLGLVTRIDLL
                     NWLRRRA"
     gene            7931..>8522
                     /locus_tag="VFB37_13500"
     CDS             7931..>8522
                     /locus_tag="VFB37_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZO23513.1"
                     /translation="MKHESRDQHFATRVIHGGQRPDPLTGAVMPPIYATSTYVQSSPG
                     VHKGYEYSRTSNPTRDALQAALANLEGGAAGYAFASGMAATGTVLELLDSGSHIIAMH
                     DLYGGSYRILENVRKRSAGHDVSFVDLTNPAALEQALRPSTRMIWVETPTNPLLKLVD
                     LSAVAKIARRHGILSVCDNTFATPFLQRPLQHGFDIV"
BASE COUNT         1490 a         2768 c         2781 g         1483 t
ORIGIN      
        1 gagcacgtgc acgaggcctt tgccgaaatc gagagctcgc gccagcgcct gatgcaaagc
       61 ggtatccgag cgggcatcca cgtcgatctc cgcgaccggc agctcgatgg tgtgctcctt
      121 gaagcgcgac agccttggcc agcgggcggt cgaggtattg gcgccatcga cccgcagcca
      181 cttgaatccc tttttggcgg cccacttggc cagatcggtg tagtagccct tgcgcgccag
      241 caccagtggg gcgaggaggg caatgcgctt gcccttgaag tcgcgcagca ggcgcaacgc
      301 gatggagccg gcaccctgcg gctcgatggc cacgttgcag tccggacagt actgggtccc
      361 gagcttgacg tagagcagtc tcaggaagtg gtagatttcc gtgagtgttg caaccgtgct
      421 tttgggtccg ccgcggctcg tgcgctgctc gatggcgacg gtcgggggaa taccgaagat
      481 ggcatcgacg tcagggcgtg cggccggttg cacaaactgg cgcgcatatg cgttgagcga
      541 ttccaggtag cgacgctggc cttcattgaa caggatgtcg aaagcgagcg tcgacttgcc
      601 cgatcccgac accccggtga tcacggtgaa tttgtcgcgc gggatctcga cgtcgacgtt
      661 cttgaggttg tgctcgcggg cgttgtgaat gacgatcgag ttccggccgc gcacgttatc
      721 ggcgcgccgg tagagcagcg caggctcggc gactgcatgg gccgcgcccg cggccctgat
      781 ctcctgggcc ggaagcggct ccgaggacag ggctctctca taggcggcga gggcctgccc
      841 ggtgaaggag cccggatgcc tggcgacctc cgcaggagcg ccggtacaga cgatctgccc
      901 gccgccgtca ccgccttcgg gcccgagatc gatgatccag tcggcagcgc ggatcacgtc
      961 aagattgtgc tcgatgacca gcagcgaatg gcccgcggcg agcagcttgc gaaaggcccg
     1021 cagcagcttg cccacatcct cgaaatgcag gcccgtggtc ggctcatcga acaaaaacag
     1081 cttgccgcga tgggtggtgc tggagaggac cgagccggcc tcggcgagat gtccggcgag
     1141 cttgagccgc tgggcctcgc ccccggatag cgtgggcacc ggttgcccca gcttgacgta
     1201 ctcgagaccg acatccgcca gcggcgcaag tcgcagcgcc agctcgcggg agtccgcaaa
     1261 gaaggcgagc gactcgctca ccgtcatctc cagaacgtcc gcgatactcg cgcgccggcc
     1321 atcggcgccc tcgcgcttga tgtcgagtat ctcgtcacga tagcggcggc cgttgcaatc
     1381 gggacagcgc agatagacat ccgagaggaa ctgcatctcg acatgctcga atccgttgcc
     1441 gctgcaggcc ggacagcggc cgttgccgga gttgaagctg aacgtgccag cggtgtactt
     1501 gcgttcctga gcggcgggct cggcggcgaa cagcgcacgg atcgagtcga aggcaccgat
     1561 gtagctggcc ggattggagc gggtggtcct gccaatcgca ttctggtcga ccatcacgac
     1621 atcatcgatc agctcggccc cccgcagccc ggtgaactcg ccgggcgtct cggtcggctt
     1681 gccgcgatac ttcagcagcg ccgggtaaag cacatccttc accagcgtgg acttgcccga
     1741 gccggacact cccgttacgc agacgagacg tttgagaggg atgcggacgt cgataccttt
     1801 gagattgtgc tcactcgcgc cctccagctc cagccacctg ttggatcgag cctccgccac
     1861 gacctctccc tgggccgacg cagcctggcc tgagacagga tcaatgccag cggggggtct
     1921 tgcgagtgca gaggcggcgc tggatctcgc accttcggcc ggtgcgccca ctctccggcg
     1981 gccgctgagg tattcaccgg tgagggacgg tgctccctgg cggatctcgg cggggcttcc
     2041 gaagaacacg atctctccgc cgcgctcgcc cgcacccggg cccatgtcga gtatgcggtc
     2101 cgcctccagc atgatctgcg ggtcgtgctc aaccacgacg agcgagttgc cggcgtcgcg
     2161 gagtttcttc atcacatgga tcacgcgctg catgtcgcgc ggatgcagac cgatcgaagg
     2221 ctcatccagg acgaacagcg tgttgacgag cgaggttccc agcgccgtcg tcaggttgat
     2281 ccgctgcacc tcgccgcccg acaaggtgcg cgactggcga tcgagagtca ggtacccgag
     2341 accgacatcg accaggtagc cgacacggcc gcgaatctcg ccgagcagaa gatcggtggc
     2401 ctcatccaga ggctgcggca ggcgcagtcc ggcgaagaac tcgcgaaccc gctcgagcgg
     2461 cagcaacatg agatcgtgga gcgacaggcc gggcggccgc tgatcgctcc actgcgcgcc
     2521 ctggggccga aacgcggaag atccgcgcag ggctgcgtcc gtcagttgct ggctgcccaa
     2581 ccgccacaga agagcttcgc tcttcagacg tgccccgcca caagcggcgc acggcgtata
     2641 ggcgcgatag cgcgagagga gcacccggac gtgcatcttg tacgccttcg tctcgagcca
     2701 ggcgaagaag cgcttcgcgc cgtaccagac tcctttgctc cagtcgccct cgccttcgat
     2761 cacccatttg cgccgctcct ctcccagctc ccgccacggc gtatccagcg gaatgcagcg
     2821 cttcctggcg aacttcacca gatcgtcctg acactccgca taggacttgg tctgccaggg
     2881 cttgatggcg ccaccgcgca acgacttgct ctcgtccgga atcacgagcc cgtaatcgat
     2941 gccgatcgtg cggccgaagc cccgacagct ttcgcaggcc cccaccggcg agttgaacga
     3001 gaacaggctc ggcgtgggct cgcgatagga cagatcgcac tcggcacaat gaagctgact
     3061 cgagtagcgc caggtttgca gcgctcgagg cggatccgac tcgtcaacca catgtacatt
     3121 gaccttgccc tggccgacac gcagcgcggc ttcgagggac tcgagcacgc ggccgcgctc
     3181 gacaccgccg gcgcgaaacc gatcctgcac gacctcgagg atcagcggcg cttccaccgc
     3241 gcgcttgcgt cgccccttct tcgcggcggc gggcggcgca gcgggcgccg tcagggcggg
     3301 gccgaagaag cgcgtgtagc cctgcttctg gagcagcgcg cgcacttccg tctctgtgaa
     3361 attccgcggg atcggcaccg gaaagctcag cagcagccgc ggatccccgg cctctcgtgc
     3421 acggccgagc agatcggcat gaatgctctc ggaagaatcg cggcgcacgg gcttgccgca
     3481 gcagcggcag tgcagagccg ccgtcctggc aaacagcagc ttcagatggt cattcagctc
     3541 ggtcatcgtt ccgaccgtgg agcgcgatgt ccggaccgga ttggtctgat cgatggcaat
     3601 ggccggcgga atacccgaga tagactccac ctgcggtttg tccatgcgat ccaggaactg
     3661 ccgcgcgtac ggagagaacg tctcgacgta tcgccgctgg ccttccgcgt acagagtgtc
     3721 gaacacgagc gaggacttgc ccgaaccgga cacgccggtg accacgacga gctccttcag
     3781 gggaatgtcc aggtcgatgt tcttgaggtt gttctggcgc gcgccgcgca ccagaattgc
     3841 ctccggtgtg tcgcgcgccg ggattccctg ctgcgcagct ccgctgctca tccgtgcaag
     3901 cctctcaggc atgcgcgcct cggtttcgct cagtcctcat cattgcatca cctggttcaa
     3961 agaagcgggc cggtcgcagg cgagtgcccg ctgtggcgcc ggtgaattcc ctggcgcgaa
     4021 ccgggcattc taaatgaatt tgacgcgact tcaggggtgg ctcggctccc aggcctgggc
     4081 tgcccggctc ccaaaggccg tttctcgaag gcaaagcccc cggggccagc agccggtcgc
     4141 cggaacaacc aggcttccag aacctcccgg gcctgcgaag gcttgggatg ccgcgcgccg
     4201 ccctgccaac aagcgagcgg tcgcccccgg cgggtgcagc ttgcttccgg caaggcgccc
     4261 accatgcggc ccctgggaag cttgggccga ttcatgcatc cgcaagcgtg gccggcacgt
     4321 attggcaccg ttgatccgga tcgtctccgg tttgcgtcgc cctggcgcga caaacgctgt
     4381 ggcggctgtg cgggtggggg tagaatgcgc ccgcagagcc aaatggccgc cagacccgtg
     4441 cattggtgag gcgtgagggg gccgctccgt ggcgcagctg tgcgcggggc ggtgcagtcg
     4501 gacggccggc cggctcgatt caggtcaact tgcgaaatcc atcgttaacg accaggagaa
     4561 cttcgaatga actctagaaa gatgtccagc atcgccttgg ccgccgccgc tgcggtgatg
     4621 ttctcgaccg cgtttgccgg caccgcgagt gcggccgatg cgaaggtgaa gtgtgaaggc
     4681 gtcaactcct gcaagggcca gtccgcctgc aaaggcgcga acaactcctg caagggacag
     4741 aactcctgca agggtcaggg ctttctcgag atgacgcagg cgaagtgcga cgcggccaag
     4801 gccaaggctg gcaagtaagc gtcaactagc gtatggactg cggccgggca ccgccttcgc
     4861 gcggacccgg cccgcagttt tcccatgact ctgctgcgcc cttccctcgg cttcggcctc
     4921 ggcctgcggg tcgatcatta cgaagccatt ctcgcggacc ggcccgcgat cgactggttc
     4981 gaggtgctga ccgagaacta cctggtcccg ggtggcaagc cactcgacta cctcatgcgc
     5041 atccgcgaac gctaccgggt tgccatgcac ggcgtgtcca tgtcgatcgg cagcactgcg
     5101 cccctggatc gatcctatct gacgaagctc aaggcgctgg ccgcgagagt cgagccggaa
     5161 tggatctcgg atcatctttg ctggaccggc gtcggtggcc acaacatgca cgatctgctg
     5221 cccttgccgt acaccgagga ggcactggct aacgtcgtcg agcgcgtcag gacggttcag
     5281 gacatgctgg gccggcgcat tctgctggag aacgtctcga gctatgtgag ctaccacgac
     5341 tcgcaactca ccgagtggga cttcctgcgc gagatcgccg aacgagccga ttgcctgatt
     5401 ctgctcgacg tcaacaacat ctatgtcagc agcgtgaatc acgagttcgc accgctcgag
     5461 tatctgaatg ccataccgat cgagcgcgtg cagcaattcc atctggccgg ccacgagaat
     5521 cacggcactt acctggtcga tacgcacgac catccggtac cggatcccgt ctgggagctg
     5581 tactgcgcgg ccgtacagcg attcggcaac gtctccacca tgatcgagcg tgatgccaat
     5641 attcctccac tggaggagtt gtgcgcggag ctggatgcgg cacgcatcct ggcagagcgc
     5701 accctggctc gcgcagcgtg agcggcgcga cggtgccgct cgcggcagtc atcgcgtgag
     5761 caggctggcg cacatacagg atgcatttca gcgcttcctg attcagcgtg ctccgggcat
     5821 cgaggcccat gtcgtgggaa gcgaacgcgt gtccgtcgcc acgcgactgg gaatttacgg
     5881 cgatggctac cgcgcacgcc tcatcgaagc gatgcagagc acgtttccgg tgctggcaac
     5941 actgctcggg gaggttgatt ttgcgaacct tgccggccag tacgtggaca ctcacgtctc
     6001 ctcattctct tccatccggt actacgggga tgagttggcg gcgtttctcg cgaccgatgc
     6061 cgagtattcc caggtgccgc tgctcgccga gctcgcgcag tgggaatggg ccatggcggg
     6121 ggccttcgat gccgccgatg tcacgccgct ggatgtgaac gcctttgcgc agttgccgcc
     6181 cgcgcagtgg gcggagctgc gcttcgactg gagcccctca acgcaggtgc tgccgctcga
     6241 atggaatgtg gcgaatctgt ggaaggcggt gacggaagac caggagcggc ccgagccggc
     6301 cgtcacgccc gagccggtaa actggttgat ctggcggcac gagctgcaga tctactttcg
     6361 ttcgttgccg acggacgagt cggcggccct cgccgcctcg cgggcgggtc agaacttcgg
     6421 cgagctctgc gtgctcttgt gtgcacacct cgaggaagac gaggcctcgc tgcgcgcggc
     6481 gtcgctgttg cgcggctggg tacaatccgg cctcatcgtc ggcgtcgccc ggccgcccta
     6541 ggagttgcgc atgtcaccgc aagtgtgcgg caacgtgttg gagatgatcg gccacacgcc
     6601 tctcatggcg gcgcgccatc tggacacggg gccctgtgag ctcttcctga agctggagaa
     6661 ccagaatccg ggcggttcta tcaaggaccg cgtgggcttg tacctcatcg aagcggctga
     6721 gcgggatggc aggatccgtc cgggtggcac gctcatcgag gcgaccgctg gcaacaccgg
     6781 cctggggctg gcgctggtgg ctgcccagaa gggttatcga ctgctgctgg tgattccgga
     6841 caagatgagc caggagaaga tctttcacct caaggcgatg ggcgccgagg tgatgctgac
     6901 gcgctccgac gtgagcaagg gccatccgga gtactaccgc gatctggccg agcggctggc
     6961 gcgtgagacg ccgaacgcct tctacgtcaa tcaattcggt aacccggcca atccccgcgc
     7021 gcacgaggag acgaccgcgc cggagatctg ggagcagatg ggccacaagc tcgatgcggt
     7081 catctgcggg atcggcaccg gtggcacgct gaccgggctg tcccgatatc tggcgcgcga
     7141 ggcgcccact gtcgagatcg tgctggccga tccggccggc tcagtgctcg cggccttcgc
     7201 caggaccggc cagattcctg ccgagcatgg cagctggctc gtggagggca ttggcggcga
     7261 ctcgattccg ccggtcgccg atctgtctcg tgtcgggtcc acctacacga tcccggatgc
     7321 cgaggcgttc cagacctgcc gggagctgct ccgccgcgag ggggtcatcg ccggcacatc
     7381 gactggcacg ctgctggcgg ccgctctgcg ctactgccgc gagcagaaat cgcccaagcg
     7441 tgtcgtgacg ttcgtctgcg atagcggcaa caagtacctg tccaaggtct acaacgatta
     7501 ctggatgctg gatcagggat tcatcgagcg cgagcgcttt ggtgatctgc gcgatctcat
     7561 tgcccgccgg cactgggagc acgcggccgt cacggtgaat tccaccgagc ccgtgctggc
     7621 agcgctgcgc cggatgaagc tctacgatgt ctcgcagctt ccggtaatgg agcgcggccg
     7681 catcgtcggc atcgtggacg aggaggacat cctgctcgag gtgttcgcca atccggacca
     7741 tttcaatgag ccggtcacca aggcgatggc acgcggcctc gtcaccgtgc ccatcgacgc
     7801 gcccgttgcc cagttgatgg agatcttcaa gcgcggcatg gtggccatcg tggtgaaaga
     7861 cggcgaattc ctcggtctgg tgacccggat cgacctgctc aactggctgc gccgccgcgc
     7921 ctgagtcgat atgaagcacg aatcgcgcga tcagcacttc gccacgcgag tcattcacgg
     7981 cgggcagcgg cccgatccgc tcaccggcgc tgtgatgccg cccatttacg cgacttccac
     8041 atacgtgcag tcgagccccg gggtccacaa gggctacgag tactcgcgca cctccaatcc
     8101 gacacgcgat gcgctgcagg cggccctggc caatctcgag gggggcgccg ccggatatgc
     8161 cttcgcatcg ggcatggcgg caaccggcac ggtactcgag ctgctggaca gcggctcaca
     8221 catcatcgcc atgcatgacc tgtacggggg cagctaccgc attctggaga acgtccgcaa
     8281 gcgctccgcc ggccatgatg tgtccttcgt cgatctgacg aatcccgcgg cgctcgaaca
     8341 ggccctgcgc cccagcaccc ggatgatctg ggtggaaacg ccgaccaatc cgctgctcaa
     8401 gctcgtggat ctaagcgcgg ttgcgaagat tgcccgccgc catggaatac tgagcgtctg
     8461 cgacaacacg ttcgcgaccc cgttcctgca gcgccccttg caacacggct tcgacatcgt
     8521 gg
//
LOCUS       DASRHN010000337         9599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1106044, whole genome shotgun sequence.
ACCESSION   DASRHN010000337 DASRHN010000000
VERSION     DASRHN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106044"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /gene="shc"
                     /locus_tag="VFB37_13505"
     CDS             complement(<1..1387)
                     /gene="shc"
                     /locus_tag="VFB37_13505"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZO23514.1"
                     /translation="MNRNVPALDEVVEEGGNARKLRAPGVGRLAQQSGLDRGIDAARA
                     ALLQSQSASGSWLFELEADCTIPAEYILMMHYLDEIDPVLQTKLAVYLRSHQAEHGGW
                     PLYHGGRLDISCSVKAYFALKLAGDSPDEPHMRRARAAILERGGAARSNVFTRIALAL
                     FGQVPWRAVPYIPVEIMLLPRWFPFHLDKVSYWSRTVMVPLLILCTYKPRAKNPRQVQ
                     ITELFTTPPAQERHYFRLPEGRGRLLARAFLLLDHVARLIDPLIPSLMRQRATRRAEQ
                     WVLERLNGEEGLGAIFPAMVNALEALVLLGYPENDLRRATAKRALQKLLVVHPSSAYC
                     QPCVSPIWDTALASLALQEAGGSEAATATERALRWLGTKQLLDEPGDWRVKRPQLAGG
                     GWAFQFANPHYPDLDDTAVIAWAMQRSAESEHGWRLERCLNWLVGMQSHNGGFAAFDV
                     DNVHYSLNQIPF"
     gene            complement(1384..2553)
                     /locus_tag="VFB37_13510"
     CDS             complement(1384..2553)
                     /locus_tag="VFB37_13510"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HZO23515.1"
                     /translation="MSPDPVKMDSGIRDEDYQDQILPHVSRTFALTIPQLPPALRTPV
                     TSAYLLCRIADTIEDEPALPPAATLILLQRFVAVVAGTEDAAPLARDLAPQLSGQTLP
                     AERDLVLNMARVVRVTARFPEPQRLAIQRCIEIMCGGMHRFQQTASVRGLPRLSDLDD
                     YCYYVAGVVGQMLTELFCDYSAQIRQKRAALHEVAVSFAQGLQMTNILKDVWEDRSRG
                     ACWLPQEVFSRHGVDLAQISATAPDPRFDAALHELTGVAHAHLRNALAFTLLIPSRET
                     GIRRFCLWAIGLAVLTLRRIEAKPGYTCGAQVKVSHTAVAMVRLLTQLSVRNDWMLRR
                     LFDRAAAGLPLASAALVRRPLRPILRPQPQEEYANSTHHVPLERAAHRSEGRSVR"
     gene            complement(2704..3648)
                     /locus_tag="VFB37_13515"
     CDS             complement(2704..3648)
                     /locus_tag="VFB37_13515"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZO23516.1"
                     /translation="MNDASSARLAPSKEAAGNPVFTAQLAAWQNRIEAALAARLPQPA
                     LTPSRLHEAMRYSVLGGGKRIRPALLFATARVLGRSEEEVEPAACAIELIHVYSLVHD
                     DLPAMDDDDLRRGRPTCHKAYDEATAVLVGDALQTLAFEVLARDPALPASAAIRLRLI
                     ELLAQASGTSGMAGGQAIDLAVQGQQLQIGQVEEMHSRKTGALIRASVLMAAACVPAL
                     EARWDAALAGFAAPIGLAFQIQDDLLDVLGDVATLGKATGADNERAKPTYPSVVGIPA
                     SQEKVRLLHQEALEALRPFGSRAEPLRALAQWLLARSY"
     gene            complement(3666..4865)
                     /gene="hpnH"
                     /locus_tag="VFB37_13520"
     CDS             complement(3666..4865)
                     /gene="hpnH"
                     /locus_tag="VFB37_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZO23517.1"
                     /translation="MSVPLLQAYRVGRYIAGRRLRGVKRYPLVLMLEPLFQCNLACAG
                     CGKIDYPKETLQKRLSVEDALRAVDECGAPMVSIPGGEPLIHKEMPQLVAGIVARRKF
                     VYLCTNAILLPKHIDDYRPSPYLTFSIHLDGSRERHDESVCQEGVFDKAVAAIRLARS
                     KGFRVTVNCTLFSGENPDHVAAFFDEAMALGIEGITVSPGYSYHHAPRQDVFLGRSAS
                     KRLFREIFKRGRRDTPVAEGPGASRRSTRSRWSFNHSALFLDFLAGNQAYQCTPWSTP
                     TYNLFGWQRPCYLLTDEGYAPTFKSLMEDTAWDHYGVGRNPACDNCMAHCGFEGTAVN
                     DAFAHPIKALGVALRGPRLTGPMAPDPPILYGEGARRSHTVATVAVADVKRANREPDA
                     RQPSELR"
     gene            complement(4882..5466)
                     /gene="idi"
                     /locus_tag="VFB37_13525"
     CDS             complement(4882..5466)
                     /gene="idi"
                     /locus_tag="VFB37_13525"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZO23518.1"
                     /translation="MDVERSSRSCADVETADSESLILVDEADREVGHLSKVQCHQGRG
                     VLHWAFSLLIFNGAGDLLLQQRAASKRLWPLYWSNSCCSHPRRAESMEVAVHRRLHEE
                     LGLRCPLHFLYKFQYQAQFDALGAEQELCSVFVGRSSEPVMVDRNEIHAWRWISPQAL
                     QAEMTAEAGRFTPWFVLEWQRVWGEHRPQLLQLT"
     gene            complement(5602..6480)
                     /locus_tag="VFB37_13530"
     CDS             complement(5602..6480)
                     /locus_tag="VFB37_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO23519.1"
                     /translation="MRFVSFDSQGQRGLAVADGKGGLRGLLASEAGFPGDLDALIPRG
                     SEALRQAGKLLLDGSCFQQDEVRVLPPLAAPPKIICVGLNYREHTRESGFQAAEYPSL
                     FVRFRSCLIGHRAALVRPRVSRQLDYEGELVAVIGQGGRHIPRARALEHVVGYSIFND
                     ASIRDYQMRTSQWTIGKNFDGTGAFGPWLVTSDELPPGAAGLQLQTRLQGRVMQNAST
                     SEFIFDVATLICLLSEAFTLEPGDLIVTGTPSGVGFARKPPVFMQPGEVCEIEIEGIG
                     TLINPVADEEPGEGRS"
     gene            complement(6501..7859)
                     /locus_tag="VFB37_13535"
     CDS             complement(6501..7859)
                     /locus_tag="VFB37_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO23520.1"
                     /translation="MSAADAVGSEHRSLGLGMCVALVVGNIIGVGIFALPASLAPYGL
                     NALTGWLITLVGCTFITITFAVLPRAFPRDDGPYAYAQRAFGDGAAFFVMWCYWVSTW
                     MTNATIAIAVVGYFTVFFPALNTNPWLPPITALSLLWIFVAINLRGARTAGWVQVLTT
                     ILKLCPLVGVICLGAWVLFAHPSAYTHNVPPNAASWGEVSSASTLALYAMLGIECATI
                     PACRIKDPQRTIPAATIIGAAVTALIYVGVSLIPMLLIPQKELAASSAPFADLFRRVL
                     GPSSGEILALFIIIGGLGALNGWTLLLGEVTQGLARHGRFPALLARENSHGAPVFAFV
                     ITGLVASAVLLSNYDQSISSGFTFMSVVVTATNIPMYFVCGLAITVLAWRGRIPAKPH
                     QAFGWAVAGFCAAAYCAWASVGIGLRPLLYTLGLSAVGIPVYLTFGHLRRRTPVTSAV
                     TD"
     gene            complement(7865..9190)
                     /locus_tag="VFB37_13540"
     CDS             complement(7865..9190)
                     /locus_tag="VFB37_13540"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HZO23521.1"
                     /translation="MPSRHILTELPGPKARALLERDQAVSSPSYPRDYPFVMARGRGV
                     EVWDVDGNRFLDFAAGIAVCSTGHAHPEVVQAIKAAADDFLHISSDYWHERMTRLAER
                     LNSLDPLGEPAQILICQSGTESVEGALKLARYVTGRPRFIGFLGGFHGRSMGSLAFTA
                     SKYTQQAGFFPTMPGVTHVPYPNTYRPLFAGEDQGAATLDYIENVLFQSNLPAAEVAA
                     VLVEPIQGEGGYLVPPAGFLQGLRQLCDRHGILLIFDEVQSGIGRTGRMFASEHWGVR
                     PDIMTLAKGLGSGLPIGVVVARRTHMEKWKRGAHGNTFGGNPVCCAAALKTLDLVEQS
                     YAANAAEVGEYFLGRLRELQKRFECIGDVRGKGLMLGAELITDRDTRKPARALCQRVL
                     TRAFHNGLLLLSCGASTLRFIPPLVASRDDVDEALGMIEAALTEALVEA"
     gene            complement(9193..>9599)
                     /locus_tag="VFB37_13545"
     CDS             complement(9193..>9599)
                     /locus_tag="VFB37_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO23522.1"
                     /translation="HPLSCAAGVAAIESYQEEGLIARSRALGTSMLARLRQMQERHRV
                     IGDVRGGEGLFAVIELVRDRATRQPPAPWPQMHAALKGLLAAGLQAGVSFAARGNLIL
                     LAPPLVIEQSELTEGLDLLEVLLERAAPGLSS"
BASE COUNT         1815 a         3171 c         3061 g         1552 t
ORIGIN      
        1 cgaatggaat ctggttgagg ctgtagtgca cgttgtccac atcgaaggcg gcaaacccgc
       61 cgttgtggct ctgcatgccg accaaccagt tgaggcatcg ctcgagtcgc cagccgtgct
      121 cgctctcggc cgagcgctgc atggcccagg cgatgaccgc ggtgtcatcc agatccgggt
      181 agtgaggatt cgcgaactgg aaagcccagc cgccgcctgc gagctgcggc cgcttgaccc
      241 gccagtcccc gggctcgtcg agaagttgtt tcgtgcccag ccatctcagg gcgcgctccg
      301 tggccgtcgc cgcctcgctg ccgccagcct cctgcaaggc gagactcgcc agtgccgtgt
      361 cccagatcgg tgacacgcac ggctggcaat acgcgctcga tgggtgcacg acgagcagct
      421 tctgcagggc tcgcttggcg gtggcccgtc gcaggtcgtt ctcggggtat ccaagcagca
      481 cgagggcctc gagcgcgttc accatcgccg gaaatatggc gccgagtccc tcctccccgt
      541 tcaacctctc gaggacccac tgctcggcgc ggcgagtcgc gcgctgtcgc atgagcgacg
      601 gaatgagcgg gtcgatcagc cgggcgacat gatcgaggag caaaaacgcg cgagccagca
      661 gcctgccccg gccttcgggc agccggaagt agtggcgctc ctgcgccgga ggcgtcgtga
      721 aaagctccgt gatctgcacc tggcgcggat tcttcgcccg cggcttgtag gtgcacagga
      781 tcagcagcgg gaccatgacc gtccgtgacc agtaggaaac cttgtcgaga tggaaaggga
      841 accagcgagg cagcagcatg atctcgacgg ggatgtaggg gactgctcgc cacggaacct
      901 gtccgaagag cgccagcgca attcgtgtga agacattgga ccgggccgcc ccaccacgct
      961 ccaggatcgc cgcgcgcgcg cgccgcatgt gcggctcatc gggagagtcg ccggcgagct
     1021 tgagcgcgaa ataggctttc acgctgcagc tgatatcgag cctgcctcca tgatagagcg
     1081 gccagccgcc atgctcggcc tgatgtgatc tgagataaac cgcgagcttg gtttgcaaaa
     1141 ccggatcgat ctcgtccagg tagtgcatca tcaggatgta ttcggccggg atggtgcaat
     1201 cggcctccag ctcgaacagc cagcttccag aagcgctctg cgactgcagc agcgcggcac
     1261 gcgcggcgtc gataccacga tccaggcccg attgctgcgc cagccgcccc acaccgggcg
     1321 cgcgcagctt gcgcgcattg ccgccctcct ccacgacctc atcgagcgcg ggtacattgc
     1381 gattcaacgg acagatctcc cttcggatcg atgcgcggcc cgctcgagcg gaacgtgatg
     1441 cgtgctgttg gcgtattctt cctgaggttg aggacgcagg atcggccgca gcggacgacg
     1501 tacgagcgca gctgaggcca ggggcaatcc ggccgcggcg cgatcaaaca gccgtcgcaa
     1561 catccagtca ttgcgcaccg acagctgcgt caacaggcga accatcgcca cggccgtatg
     1621 ggagactttg acctgcgcac cgcaggtata gccgggcttc gcctcgatcc tgcgcagcgt
     1681 gagcaccgcc agcccgatag cccacaggca gaagcgccgg atacccgttt cccgtgatgg
     1741 gatgagcagc gtgaaggcga gtgcgttgcg cagatgagca tgggccacac ccgtcaactc
     1801 atgcagggcg gcatcgaagc gcgggtccgg cgcagtggcc gaaatctggg cgagatccac
     1861 gccgtgacgg ctgaagactt cctgcggcag ccagcaggct ccgcggctgc gatcctccca
     1921 tacatccttg aggatgttcg tcatctgcag gccctgagcg aaggagacgg cgacctcgtg
     1981 cagcgccgcc cgtttctggc gaatctgcgc cgagtagtcg cagaacagct ccgtgagcat
     2041 ttggccgacc accccggcca cgtagtagca gtagtcatcc agatcgctca ggcgcggcag
     2101 gccgcggacg ctggcagtct gctgaaagcg atgcatgccg ccgcacatga tctcgatgca
     2161 gcgctggatt gcgaggcgct ggggctcagg gaaacgcgca gtgaccctga cgacccgcgc
     2221 catgttgagc accaggtcgc gttcggccgg taaggtctgc cctgagagct ggggcgccag
     2281 atccctcgcc aggggagcgg catcctcggt accggccacg accgccacga agcgctgcag
     2341 cagaatcagc gtcgccgcag gcggcagcgc cggctcgtct tctatggtgt ccgcgatgcg
     2401 gcacagcaga tacgcgctcg tgaccggggt tcgcagcgcc ggcggaagct gtggaatcgt
     2461 cagtgcgaac gtgcgggaaa catgcggcag gatctgatcc tggtagtcct cgtcacggat
     2521 gccggaatcc atcttgaccg ggtctggtga catcgtccga acgggcctct gggctgcggg
     2581 cgctatccac ccctcaacgc ggcagacttt aacatggcgg acatatggca accgcgcagg
     2641 ccccggccca cggcctaatt tgctttgcaa tgtaaagtta cagcccggag tcgtggtgcg
     2701 gcctcaatag ctgcgggcga gcagccactg agccagcgcg cgcagcggct cggcacgcga
     2761 gccgaagggc ctgagcgcct ccagggcctc ctggtggagc agacgcactt tctcctggga
     2821 ggcggggata ccgaccaccg aggggtatgt cggcttggcg cgctcgttgt ccgcacccgt
     2881 ggccttcccg agtgttgcaa catcccccag gacgtcaagg aggtcatcct gaatctgaaa
     2941 cgccagaccg atgggggccg caaagccggc gagcgccgca tcccatcgcg cctcgagcgc
     3001 gggcacacag gcagctgcca taagcacgct cgcccggatc agcgcgccgg tcttgcgtga
     3061 gtgcatctcc tcgacctgac cgatctgcaa ttgctgcccc tgaacggcca gatcgatcgc
     3121 ctggccgcca gccatgccag aggtaccgct cgcttgcgcg agcagctcga tcagcctcag
     3181 ccggatagcc gctgatgcag gcagcgccgg atctcttgcg aggacctcga aggcgagcgt
     3241 ctgtagcgca tcccccacca gcacggcggt cgcctcatcg taagccttgt gacaggtcgg
     3301 tcgtccgcgg cgcaggtcgt catcatccat tgccggcaga tcatcgtgga ccagggagta
     3361 gacgtggatc agctcgatcg cgcacgcggc cggctcgacc tcctcttcgg accggccgag
     3421 gacgcgggcc gtggcaaaca gcaacgccgg ccgaatgcgc ttgccccctc cgagcacgct
     3481 gtagcgcatc gcctcgtgca agcgcgacgg ggtgagcgca ggttggggca gtcgcgcggc
     3541 taacgccgct tcgatgcggt tctgccacgc cgccaattgt gcagtgaata ccgggttgcc
     3601 ggcggcttct ttcgatgggg ccagtcgagc actggatgcg tcgttcacgc ccgccctttc
     3661 gatgcttaac ggagctcgga aggctgacga gcgtcgggct cgcggttcgc ccgtttgaca
     3721 tccgcgaccg ccaccgtagc gacggtatgc gagcgcctcg cgccctcgcc atagaggatc
     3781 ggtggatcgg gagccatcgg gccggtcagt cggggtccgc gcagcgccac acccagggcc
     3841 ttgatcggat gcgcgaaggc gtcattcacg gcggtgccct cgaatccgca gtgcgccatg
     3901 cagttgtcgc atgccggatt tcgcccgacc ccataatggt cccaggccgt atcctccatc
     3961 agcgacttga acgtcggcgc gtagccctcg tcggtgagca ggtaacaggg tcgctgccaa
     4021 ccgaagaggt tgtacgtcgg cgtgctccag ggcgtgcact ggtaagcctg attgcccgcc
     4081 aggaaatcca gaaacagggc tgaatggttg aatgaccagc ggctgcgagt gctgcggcgg
     4141 ctggcacccg gtccctcggc cacgggcgtg tcgcgccggc cccgtttgaa gatttcgcgg
     4201 aacagccgct tgctggcact gcggccgagg aagacgtcct gtcgcggagc gtggtgatag
     4261 ctgtatccgg gagagacggt gatgccctcg atgccgagtg ccatcgcctc atcgaagaat
     4321 gcggcgacgt gatccgggtt ctcgccgctg aagagcgtgc agttgaccgt cacccggaag
     4381 cccttgctgc gcgcgaggcg gatcgccgcc accgccttgt cgaacacccc ctcctgacag
     4441 accgactcat cgtggcgctc gcggctgcca tcgagatgga tcgaaaaggt gagatagggc
     4501 gaaggacggt agtcgtcgat gtgtttcggc agcagaatgg cattagtgca cagatagacg
     4561 aacttgcgtc gcgccacgat gcccgcgacg agctgcggca tttccttatg tatgagcggc
     4621 tcgccgcccg gaatggagac catcggcgcg ccacattcgt ccaccgctcg cagcgcgtcc
     4681 tccacgctca gccgcttctg tagcgtctcc ttcggataat cgatcttccc gcagccggca
     4741 caggcgagat tgcactggaa caggggctcg agcatgagca ccagcggata gcgcttgacg
     4801 ccgcgcagtc tgcggccggc gatgtaccgg ccaacacggt aagcctgtaa caagggaacg
     4861 ctcaatcctg agactcctgt ttcatgtcag ctgaagcagc tgcgggcggt gctcgcccca
     4921 cacccgttgc cactcgagta cgaaccatgg cgtgaaccgg ccggcctcgg cagtcatctc
     4981 ggcctgcagc gcctgcgggc tgatccagcg ccacgcgtga atctcgttgc ggtcgaccat
     5041 caccggctcg ctcgagcggc caacgaacac cgagcacagc tcctgctcgg ccccgagcgc
     5101 atcgaactgc gcctggtact ggaacttgta cagaaagtgc agcgggcagc gcagtccgag
     5161 ctcctcgtgc agccggcgat gaacggccac ctccatgctc tcggcgcggc gcggatgact
     5221 gcagcagctg ttggaccagt aaagcggcca caaccgcttc gaggccgcgc gctgctgcag
     5281 cagcagatcc cccgcaccat tgaagataag caaggaaaag gcccaatgca ggacacctct
     5341 gccctgatga cattgaacct tgctcaggtg accgacttcg cggtcagcct cgtccaccag
     5401 gatgagcgac tcggaatctg cggtctcgac atcggcgcag ctgcggctgc tccgctctac
     5461 atccaccatc cctccatgat agccgtcagc tcgggcattc gccaaagtaa tcttggaacg
     5521 gccgcccgca ggctgcggtc cccgcatggg ggcctgagct tcaccattcg aaggccttcc
     5581 tggaccttcc aacccaaccg gtcagctccg accctcgccg ggctcttcgt cggcaaccgg
     5641 gttgatcaat gtaccgatgc cttcaatctc gatttcgcag acctcccccg gttgcatgaa
     5701 cacgggcggt ttgcgcgcga accctactcc ggagggcgtg cctgtcacga tgaggtcgcc
     5761 gggttccagt gtgaaggctt cgctcagcag gcagatgaga gtcgccacat cgaagatgaa
     5821 ctcggaggtc gaagcgttct gcatgaccct gccctgcaac cgcgtctgca gctgcaggcc
     5881 cgcggcgccc ggcggcagct catcgctcgt cacgagccag gggccgaacg ccccggtgcc
     5941 gtcgaaattc ttgccgatcg tccattgcga ggtgcgcatc tgataatcgc ggatggaggc
     6001 atcgttgaat atggaataac cgacgacatg ctccaatgcg cgagctcgcg gaatgtgccg
     6061 cccgccctgg ccgatcaccg ctaccagctc cccctcgtag tccagttgtc gtgagacacg
     6121 cggacgcacg agcgcggccc ggtgtccgat gagacaggag cggaagcgca cgaacaaaga
     6181 tggatattcg gccgcctgga agccgctctc acgcgtgtgc tcgcgatagt tgagcccgac
     6241 acagatgatc ttcggcggcg ccgccagcgg cgggagcacc cgcacctcat cctgctggaa
     6301 gcagctgcca tccagcagca gcttgcccgc ctgccgcagt gcctcggagc cccgaggtat
     6361 cagagcatcc aggtcccccg gaaagcctgc ctcgctcgcc agcaatcccc gcaaccctcc
     6421 cttgccatct gccacggcca ggccacgttg accctgcgag tcgaatgaaa caaatcgcat
     6481 cgcgatcgcg actccacacc ctaatccgtc acggcactcg tgacaggcgt tcggcgtcgc
     6541 agatgcccga aagtgagata gaccggaatg ccgacggcgc tcagcccgag agtatagagc
     6601 aggggccgca gtccgatgcc caccgacgcc cacgcgcaat aggctgcagc acagaacccc
     6661 gcgacggccc agccgaaggc ttggtggggc ttggccggaa tgcgccctcg ccacgcgaga
     6721 actgtgatcg ccaggccgca gacgaaatac atcggaatgt tggtggcggt cacgacgacg
     6781 ctcatgaacg tgaagccgct cgaaatggac tgatcatagt tgctcagcag cacggcgctg
     6841 gcgacgagac ccgtgatcac gaacgcaaac accggcgcac cgtgcgaatt ttcccgggcg
     6901 agcagcgcgg gaaaccggcc atggcgtgcc aagccctgcg tcacctcgcc cagcagcagg
     6961 gtccaaccgt tgagtgcacc cagtccgccg atgatgatga acagcgccag aatctcgccg
     7021 gaggacggac cgagcacgcg ccggaagaga tcggcgaacg gcgcgctgga agccgccagt
     7081 tccttctggg gaatcaacag catcggaatc aacgagacgc ccacatagat caaggcggtc
     7141 acggcggccc cgatgatggt ggctgcgggt atcgtccgct gtggatcctt tatgcggcac
     7201 gcaggaatgg tcgcgcactc gatgccgagc atggcataca gagcaagggt ggaggcgctg
     7261 ctcacctccc cccaggatgc cgcattcggc gggacgttgt gtgtgtaggc cgaggggtgc
     7321 gcgaagagca cccaggcgcc caggcagatg accccgacca gcgggcagag cttcagaatc
     7381 gtcgtgagca cttgcaccca gccagccgtt cgggctccac gcaaattgat cgcgacgaag
     7441 atccagagca gagacaacgc cgtgatgggc ggaagccacg gattggtgtt gagcgcaggg
     7501 aagaagacgg tgaaatagcc gacgacggcg atcgctatgg tggcgttggt catccaggtg
     7561 gagacccagt agcaccacat gacgaagaag gccgcgccat ccccgaatgc acgctgcgca
     7621 taggcgtacg ggccatcgtc ccgcgggaaa gcgcgtggca gaacggcaaa agtgatcgtg
     7681 atgaatgtgc agccgacgag ggtgatgagc catcccgtca gcgcattgag cccgtacgga
     7741 gccagcgagg ccggcagggc aaagatacct acaccgatga tgttgcccac gaccagcgcg
     7801 acgcacattc cgaggccgag gctgcgatgc tcgctcccga ccgcatcggc cgccgacacc
     7861 gcgctcaagc ttccaccagc gcttcggtca gggccgcttc gatcatgccg agggcttcgt
     7921 ccacatcgtc gcggctcgcg acgagcggcg gaatgaaacg gagtgtgctc gcaccacagg
     7981 acagcagaag caggccgtta tgaaaggcac gcgtcagcac cctctggcac aaggcccgcg
     8041 caggcttgcg agtgtcccgg tcggtaatca gctccgcgcc gagcatcagc cccttgccac
     8101 gaacatcccc gatacactcg aagcgcttct gcagctcgcg cagccggccg aggaaatact
     8161 cccccacctc ggctgcgttc gccgcataag actgctcgac cagatcgagc gtcttcagcg
     8221 ccgccgcgca gcacacagga ttgccgccga aggtgttgcc gtgcgcaccg cgtttccatt
     8281 tctccatgtg cgtgcggcgg gccacgacca ctccaatcgg cagccccgat ccgagtccct
     8341 tcgccagtgt catgatgtcg ggtctgacgc cccagtgctc gcttgcaaac attcgcccgg
     8401 tgcggccaat accgctctgc acctcatcga agataagcag aatgccatga cggtcgcaaa
     8461 gctggcgcag accctgcaga aagcctgcgg gcggcacgag gtaaccgccc tccccctgaa
     8521 tgggctctac caggacagcc gccacctctg cggccggcag gttgctctgg aacagcacgt
     8581 tctcgatgta atcgagagtc gccgcgccct ggtcctcgcc tgcgaacagc gggcggtacg
     8641 tgttggggta cggaacgtgt gtgaccccgg gcatcgtcgg aaagaagccg gcctgctgcg
     8701 tgtacttgct cgcggtgaag gcgagtgagc ccatcgagcg gccgtgaaaa ccgcccagaa
     8761 agccgatgaa gcggggccgg cccgtcacat agcgggccag cttgagagcg ccttccaccg
     8821 attcggtgcc gctctggcag atgaggatct gggccggctc gcccagcggg tccagcgagt
     8881 tgaggcgttc ggcgaggcgc gtcatgcgct catgccagta gtcgctcgag atatgcagga
     8941 agtcatccgc cgcagccttg atggcctgca cgacctccgg atgcgcatgg ccggtgctgc
     9001 agacggcgat gcccgcggca aaatccagaa agcggttgcc atccacatcc cacacttcga
     9061 cccctcgtcc cctggccatg acgaacgggt agtcgcgcgg ataggacggt gagctgaccg
     9121 cctggtcgcg ttccagaagg gcccgtgctt tcggtcccgg cagctcggta aggatgtggc
     9181 gggacggcat cttcaggatg atagcccagg ggccgcccgc tccagcagca cttccagcag
     9241 atcgaggcct tcggtgagct ccgactgctc gatcaccagc ggaggagcca gcaggatcag
     9301 gttgccacga gccgcaaagg acaccccggc ctgcaacccc gccgccagca gccctttcaa
     9361 ggcggcatgc atctggggcc agggtgcggg gggttgccgg gtggcgcgat cgcgtaccag
     9421 ctcgatgacg gcaaacagcc cctcaccgcc gcgcacgtcc ccgatcactc tgtgccgctc
     9481 ctgcatttgc cgcaagcggg ccaacatcga cgtgccgagc gcccgggagc gcgcgatcag
     9541 accttcctcc tgataactct cgattgcggc aacaccggca gcgcacgaca gcggatgcc
//
LOCUS       DASRHN010000338         8116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2423910, whole genome shotgun sequence.
ACCESSION   DASRHN010000338 DASRHN010000000
VERSION     DASRHN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423910"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VFB37_13550"
     CDS             complement(<1..1090)
                     /locus_tag="VFB37_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZO23523.1"
                     /translation="MSSVLPHMRSTLPGMGSTLRAALLIAACSVAISATAQQPARPAY
                     KHLGVGSCSSSVCHGKTKAQQGRDVALNEYTIWSSPDDFHSKAYRTLSSPRSQAIAAK
                     LGLASASTATICLDCHADNVPVAQRGPKFQLTDGVSCEACHGGAEKWIETHTQKSAGH
                     RDNVSKGMYPSEQPLARAELCLSCHMGTRDKFATHVIMGAGHPRLYFELEVFSYNQPP
                     HYVVDEDYIARKGRIEQMNLWVTGQIENARRYLELLQTRLTVPDSFLPEFALYDCFSC
                     HHPMDKQRWTRARAGAGIQPGTLPLQRDYLVMMQAVTEVLAPGEVGQLSAASEALVRA
                     GQKDAASLRQEAQKLHAWIDGHDSWTRRQ"
     gene            complement(1107..4673)
                     /locus_tag="VFB37_13555"
     CDS             complement(1107..4673)
                     /locus_tag="VFB37_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23524.1"
                     /translation="MHANTAINLGCTDCHGGNQAAVLPQGVSRSSAQYRQVMDAAHVL
                     PRYPSEWHYPSSANPQRSYTLLNRESPEFIRFMNPSDYRVVRESCGACHREIIEASLR
                     SLHATNAMFWGGASYNNGILDSKNYILGESYSREGLGVLLKGPVPKDPHVAFDAGILP
                     QLLPLPPWESIKPGDIFRIFERGGRNASNLFAETGLPDAQGELERIEEPGRPDIRASN
                     RGPGTGARIAVPVINITKTRLNDPNLWFIGTNDQPGDYRQSGCASCHVVYANDRDPLH
                     SSVYAAAGHDGTSQTVDPTIDHTQAGHPLKHAFTRSIPTSQCMVCHMHQPNMFMNSML
                     GYTMWDYESDAAAMWPEKQQYPTSSQMRKVLDRNPEGAAVRGKWADVDFLRSVADLNP
                     QLKDTQFADYHGHGWNFRAVYKRDRAGDLLDAKGGLVADDDPQKFAKAVHLASTHVDV
                     GMQCVDCHFSQDNHGSGYIYGEVAAAVEIGCKDCHGTVSNYPNSYTSGPAALNGGVDL
                     LSLRTPDGRRRFEWIAGQLVQRSMVDPNLEWKVSLVKDSVTPGSPNYNPKAARAKLMS
                     RDTSRQNWGLDVHGDSLAHSDQNMECYSCHTSWTTSCGGCHLPIEANAKTARHQYEGG
                     DTRNYATYNPQVARDDMFMLGHRETAGGGKIAPFRSSSALVLSSTNANREHIYVQQPP
                     ISASGFSSQAFNAHYPHTERKVETKDCDDCHLSKDNDNNAIMAQLLLYGTNFVNFIGY
                     HAYVGGAGEVSAVRVTEWDEPQAVIGSYLHKYAYPDWYAEHIKHDRKLQHAESHGAGD
                     AQCLQLRGEFLYVAEGSRGMRVYDVASVGNKGISEGVITTPFSPLGDNTHVGSADATC
                     VALPTNQPINPARNQGDRMRVANQESAFHPLYNYAYITDAREGLILTNIDTLADGEPR
                     NNFLKRAVTWNPDGILNGARHLTIGGYYIYVATPGALVIVGVDDPLHPKLVAKLPLED
                     PRASALQFRYLFVTDAKGLEVVDVTHPESPQLVPNNVVQIADAHRVYVARTYAYVAAG
                     KEGLVIVNVERPEAIHLQQRFNAGGRLQDSRDVVVASTNASLFAYVADGTGGLKVLQL
                     MSPASQRKFYGFSPEPRPELIASYATHRPALSLSKGLDRDRAVDETGGQIAVFGRRGT
                     RALSTSEVHDLYLDASGKPWFVKGSEAAPRGSTPQGSTPGGR"
     gene            complement(4921..6813)
                     /locus_tag="VFB37_13560"
     CDS             complement(4921..6813)
                     /locus_tag="VFB37_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23525.1"
                     /translation="MTGSEGARRLTGARLLGPLATYLLLLGATLPEPAQADTCYRDDT
                     GRIFRRRLPGTVEVPCPSTAAPQGQPPASTGPGQGTPAEGAPSAQPGFDRGPRPAASP
                     VPLPGLTDYVESVPLPDRWRIVDELGYPHNLLDPYNRNPLKADRPFHGDWFFNLGLLS
                     DSTFEYRNLVSSVGLASTARPGSYDVFGHPGGHMLSENVSTDLTLYKGDTVFKPPDWQ
                     FRLTPVFNYNYASVDEVGLLNVDPRRGTTRSDDFVGIQNAFVEKHLRNVSDRFDFDSI
                     RIGIQPFSSDFRGFLFQDNQPGVRLFGTRDNNIFQYNIAWFRRLEKDTNSGLNDVTQP
                     LRHDDIGVANLYWQDMPVQGFTSQGTVIYNHNTEGGEQHFDTNGFLVRPAALGLELPH
                     DYAVTYLGYNGDGHFGRTNATVSFYYVTGHEEPGVFSDTRLKVSAAFAAAEISRDFDW
                     FRPRLSLLYASGDSNPYDRKATGFDAIFENPQFAGGDNSYWISQAVPLIGGGGVTLTS
                     GNGLLADLRSSKGEGQSNFDNPGVMLAGLGADLDLLPTLRVTLNVNDLYFAQTAVIAA
                     LRAQAFDDRHIGEDASVAVTYRPLMSQNIVVRAAYARLFSSSALDALFPRHNPDYFLL
                     NAIFAY"
     gene            complement(6800..>8116)
                     /locus_tag="VFB37_13565"
     CDS             complement(6800..>8116)
                     /locus_tag="VFB37_13565"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZO23526.1"
                     /translation="VDVDEAIAMAATFNFGAPADRRADQITVGGLALRYTNIEYSALS
                     TDPGSAPAYSSLSPSVGSLIAVTGYSDGHIHTGTAFGQPSSGVRPEGGADFPGQDAFV
                     LVDATNTPRFPAKAGTDGPNALTRDEVLQVLRSSFAIAEKARGQIRLPLGDTARVSIT
                     VVDTLGTPLGFIRSRDAPLFGADVALQKARTAALFSSSTAAAFLSSLPPAQYVSINAS
                     GLTSNGRVPLASYVSAAQSFLGDNRALADGVIAYSNRAIGNLSRPLFPDGIDGTRNGP
                     FSKPIGQWSVFSTGLQLDLSLNAILQHVVYAIGVPGVPDVPVGCAGVGLSVPPVSAAV
                     SITASTASAALKNGLQIFPGSVPIYRGDILVGAIGVSGDGVDQDDMVAFLGLHDASEA
                     LHGSISQASASRRADTLSPKGTRLLYVQCPQSPFLDSDQENVCDGL"
BASE COUNT         1421 a         2525 c         2697 g         1473 t
ORIGIN      
        1 actgccgccg cgtccagctg tcatgtccgt ctatccaggc gtgcagcttc tgggcctcct
       61 gcctcaagga ggccgcatcc ttctgtcccg cgcgcacgag cgcctcgctg gcggcggaga
      121 gttggccgac ctcccctgga gccaacactt cggtgacggc ctgcatcatg acgaggtaat
      181 ccctctgtag aggcagcgtg cccggctgga tgccggcgcc cgcccgggcg cgggtccagc
      241 gttgcttatc catgggatga tgacagctga agcagtcgta gagcgcgaac tccggcagga
      301 agctgtcggg caccgtgaga cgggtctgca gcagctccag ataacggcgc gcattctcga
      361 tctgaccggt gacccacagg ttcatctgct cgatccggcc cttgcgcgcg atgtagtcct
      421 catccaccac gtagtgcggc ggctggttat agctgaagac ctccagctcg aagtacagcc
      481 gggggtggcc cgcacccatg atgacgtgcg tggcgaattt gtcccgcgtg cccatatgac
      541 aggacaggca tagctcggcg cgggcgagcg gctgctcgct cgggtacatc cctttggaga
      601 cattgtcacg atgcccggcg gacttctgtg tatgagtctc gatccacttc tccgcgcccc
      661 cgtgacaagc ctcgcagctc acaccatcgg tgagctggaa cttcggtccg cgctgggcca
      721 caggcacgtt atccgcatgg caatcgaggc agatcgtggc cgtcgaggcg ctcgcgagcc
      781 cgagcttggc ggcgatggcc tgagagcgcg ggctgctcag cgttcgatag gccttggaat
      841 ggaagtcgtc aggcgatgac cagatggtgt actcgttcag cgcgacatcg cgcccctgct
      901 gtgccttggt tttcccgtga caaacgctcg aggagcagct tcccacgccc agatgtttgt
      961 aggccggccg ggcgggctgc tgcgccgtgg ccgagatcgc cacggagcag gccgcgatca
     1021 ggagcgcggc ccggagggtg ctacccatgc cgggcagggt gctgcgcata tggggcagga
     1081 cgctgctcat gttctgccgg gcgcgctcac cggccgcccg gggtcgagcc ctgcggtgtc
     1141 gagccgcgcg gggcggcttc cgaccccttc acgaaccagg gcttgcctga ggcatcgaga
     1201 tacaggtcgt ggacttcgga tgtgctgaga gcccgggttc cacgccggcc aaatacggcg
     1261 atctgcccgc ccgtctcatc cacggcccga tcccgatcca ggcccttcga caggctcagc
     1321 gccggccggt gtgtggcata gctggcgatg agctcgggcc gcggctccgg gctgaagccg
     1381 tagaactttc gctgcgacgc gggcgacatg agctgcagga ccttcaggcc ccctgttcca
     1441 tcggccacgt aagcgaacag cgaagcgttg gtggatgcaa ccaccacgtc gcggctgtct
     1501 tgcagacggc ccccggcgtt gaaacgttgc tgcagatgga tcgcttcggg acgctcgacg
     1561 ttcacgatca cgaggccctc cttgcccgct gcgacgtagg catacgtgcg cgccacatac
     1621 acccggtgcg catcggcaat ctgcaccaca ttgttcggca cgagttgcgg cgactccgga
     1681 tgggtcacat ccaccacttc gagacccttc gcatcggtga cgaacagata tctgaactgc
     1741 agcgcgctgg cccgtgggtc ctccaggggc agcttcgcca cgagcttcgg gtgcagcggg
     1801 tcatcgacac cgacgatcac gagcgcgccg ggagtcgcga cgtagatgta gtagccaccg
     1861 atggtcagat gccgcgcacc gttcaggatg ccatccggat tccaggtgac cgcccgcttc
     1921 aggaagttat tgcgaggctc accatccgcc agggtgtcga tattggtcag gatcagtccc
     1981 tccctggcat ccgtgatgta ggcatagttg tacagcggat gaaaggcaga ctcctgattg
     2041 gccacgcgca tcctgtcccc ctgattgcgc gccggattga tcggctggtt ggtcggcaaa
     2101 gccacacaag tcgcatcagc cgaaccgaca tgcgtgttgt cccccaacgg gctgaacggg
     2161 gtcgtgatca ctccctccga gatgcctttg ttgccgacac tcgccacgtc atagacgcgc
     2221 attccgcggc tgccctcggc gacgtagagg aattcgcctc gtaactgcag acactgcgca
     2281 tccccggcgc cgtgactctc agcgtgctgc aacttgcggt cgtgcttgat gtgctcggca
     2341 taccagtccg gatacgcata cttgtgcagg tagctgccga tgacggcctg cggctcgtcc
     2401 cactcggtca cgcggactgc gctgacctcg cccgcgccgc ccacgtacgc gtgatagccg
     2461 atgaaattga cgaagttggt cccgtaaagg agcagctgcg ccatgatggc gttgttgtcg
     2521 ttgtccttcg agagatggca gtcgtcacaa tccttcgtct cgaccttgcg ctcggtatgc
     2581 ggatagtgcg cattgaaggc ctgactgctg aaaccgctgg cggagatggg cggctgctgc
     2641 acgtagatgt gctcacgatt ggcgttcgtc gaggacagca ccagagccga gctcgatctg
     2701 aagggcgcga tcttgccgcc cccggccgtc tcgcggtggc ccagcatgaa catgtcatcg
     2761 cgcgcgacct gcggattgta ggtggcgtag ttgcgcgtat ccccgccctc gtactggtgg
     2821 cgggccgtct tggcattcgc ctcgatcggc agatggcagc cgccgcagct cgtggtccag
     2881 gaggtgtggc aggagtagca ctccatgttc tggtcgctgt gagccagcga gtccccatga
     2941 acgtcgagtc cccagttctg ccggctcgtg tcgcggctca tcagcttggc gcgggcggcc
     3001 ttggggttgt agttcggact gcccggcgtc accgagtcct tcaccaggct gactttccac
     3061 tcgaggttcg gatccaccat cgagcgctgc accagctggc cggcgatcca ttcgaagcgc
     3121 cgccgtccgt cgggtgttcg caggctcagc aggtctaccc caccgttcag ggcggccggc
     3181 cccgaggtgt aggagttcgg atagttgctc accgtgccat gacaatcctt gcagccgatc
     3241 tcgaccgctg cggcgacctc gccgtagatg tagccgctgc cgtgattgtc ctgcgagaaa
     3301 tggcagtcca cgcactgcat gcccacatcg acatgcgtcg aggcgagatg cacggccttg
     3361 gcgaatttct gcggatcgtc atccgctacg aggccgccct tggcatcgag caggtcgccg
     3421 gcgcgatcgc gcttgtagac ggcgcggaag ttccagccgt gtccgtgata gtcggcgaac
     3481 tgggtgtcct tcagctgcgg attcagatcg gccaccgagc gcaggaaatc cacatccgcc
     3541 cacttgccgc gcaccgccgc cccttccgga ttgcgatcga gcaccttgcg catctggctg
     3601 ctggtcggat attgctgctt ctccggccac atcgccgcgg catcggactc gtagtcccac
     3661 atcgtgtagc cgagcatgct gttcatgaac atgttgggct gatgcatgtg gcagaccatg
     3721 cactggctgg tcggaatcga tcgcgtgaag gcgtgcttga gcggatgacc cgcctgagtg
     3781 tggtcaatgg tcggatcgac cgtctgcgag gtgccatcgt gtccggctgc ggcgtacacg
     3841 ctggaatgca acggatcgcg gtcgttggca tagaccacgt ggcaggatgc gcagccggac
     3901 tggcggtagt cccccggctg atcgttggtg ccgatgaacc agagattcgg atcgttcagg
     3961 cgagtcttgg tgatattgat gaccggcacc gcaatgcgcg cgccggtacc cgggccgcga
     4021 ttggacgcgc ggatgtcggg ccggccgggc tcctcgatcc gctccagctc accctgcgca
     4081 tcgggcagcc cggtctcggc gaacagattg ctcgcgttac ggccgcctcg ctcgaagatg
     4141 cggaagatgt cgccgggctt gatgctttcc cagggcggca gcggcagcag ctgagggagg
     4201 atgcccgcat cgaaggccac gtgcgggtcc ttcggaaccg gacccttcag cagcacaccc
     4261 aggccttcgc gactgtagga ctcgcccagg atgtagttct tcgaatccag gatgccgttg
     4321 ttgtacgagg cgccgcccca gaacatcgcg ttcgtggcgt gcagactgcg cagggaggcc
     4381 tcgatgatct cgcgatgaca ggcgccgcag gactcgcgca ccacgcggta gtcggacggg
     4441 ttcatgaagc ggatgaactc gggcgactcc cgattgagca gcgtgtagct gcgctgcgga
     4501 ttcgcgctcg agggatagtg ccattcggag ggatagcgcg gcagcacgtg cgcggcatcc
     4561 atcacctggc gatattgagc ggagcttcga ctcacgccct gcggcagaac cgccgcctga
     4621 ttgccgccat gacaatccgt gcaacccaga ttgatcgcgg tattggcgtg catcgtcgcg
     4681 ttatcagtct gcgtgtgaca tgacaggcat ccgccactct tctgtgccgc catctcaggg
     4741 gactgcgtca tcggagacgg cggcgcggtc gcgtagtgcc gatcgaccgg ttcgcccgag
     4801 cgtgcgctga agggagcgag aagcgctgcg cacagacaca gcagcgctac ggccggcgct
     4861 gcacttcgcc ctcggaggcc gctcgaagca ggggttggtg acatgtctgg catcgcagcc
     4921 tcaataggca aagatggcgt tcaggaggaa gtaatccggg ttgtgccgcg gaaacaacgc
     4981 atccagagcg ctcgatgaga acaggcgcgc ataggcggcg cgcaccacga tgttctggga
     5041 catcagcggg cggtacgtca cggcgaccga agcatcctcg ccgatgtgtc gatcatcgaa
     5101 cgcctgggcc cgcagcgcgg cgatcacggc agtctgggcg aaataaaggt cgttgacgtt
     5161 cagggtaacc ctcaaggtcg gcagcaggtc gagatctgca cccaggcccg cgagcatcac
     5221 ccccggattg tcgaagttgg attggccctc gcccttcgag gagcgcaggt ctgcgagcag
     5281 gccgtttccg gaagtgagcg tcacgccgcc gcccccgatc agcggcacgg cctggctgat
     5341 ccagtagctg ttatcgccgc cggcaaactg cggattctcg aagatcgcat cgaagccggt
     5401 ggccttgcgg tcatatggat tggagtctcc actcgcatac agcagcgaca gccgcggacg
     5461 gaaccagtcg aaatcgcgcg agatctccgc tgctgcgaat gcggcgctga ccttcaatcg
     5521 cgtatcggag aacacgccgg gctcctcgtg gccggtcacg taatagaaag acaccgttgc
     5581 attcgtgcgg ccgaagtgac cgtcgccgtt atagcccagg taggtgacgg cgtagtcgtg
     5641 cggcagctcc agacccagag ccgccggccg caccaggaag ccgttcgtgt cgaaatgctg
     5701 ctcgccgccc tcggtgttgt ggttgtagat gacggtaccc tgggacgtga atccctgcac
     5761 cggcatgtcc tgccagtaga gattcgcgac gccgatgtca tcgtgccgca ggggctgcgt
     5821 cacatcattg agcccgctgt tggtatcctt ctcgagtctg cggaaccagg cgatgttgta
     5881 ctggaagatg ttgttgtcac gcgtaccgaa cagccgcact ccgggctggt tgtcctggaa
     5941 gaggaagccg cggaagtcgc tggagaacgg ctggatgccg atgcggatgc tgtcgaagtc
     6001 gaaccggtcg gacacgttac gcagatgctt ctcgacgaac gcgttctgga tgccgacgaa
     6061 gtcatccgag cgcgtcgtgc cgcgcctcgg atccacgttg agcagaccca cctcatcgac
     6121 cgaggcgtag ttgtagttga agaccggtgt caggcggaac tgccagtccg gcggcttgaa
     6181 gaccgtatcg cccttgtaca gggtgagatc ggtcgagacg ttttccgaga gcatatgccc
     6241 gcccggatgg ccgaagacat cgtagctgcc agggcgcgca gtggaagcga gacccactga
     6301 ggagacgaga ttgcggtatt cgaacgtcga gtctgacagc aggccgagat tgaagaacca
     6361 gtcgccatga aagggccggt cggccttgag cggattgcgg ttgtaggggt ccagcagatt
     6421 gtgcgggtag ccgagctcat cgacgatgcg ccagcggtcg gggagcggca cggactcgac
     6481 gtagtcggtc agcccgggaa gcggaacggg cgaggcggcc ggccgtggtc cgcgatcgaa
     6541 gccgggttgc gccgagggcg cgccctccgc cggggtgccc tgtccgggtc cggttgaggc
     6601 aggaggttgg ccctggggtg ccgcggtgct ggggcaaggc acttcgacgg tccccggcag
     6661 gcggcggcgg aatatccgac cggtgtcgtc gcggtagcag gtgtccgctt gcgccggttc
     6721 gggcaatgtt gcaccgagca gcagcagata ggtcgcgagc ggcccgagca gccgcgcccc
     6781 cgtcaggcgg cgtgcgccct cagagcccgt cacagacatt ctcctgatcg ctgtcgagga
     6841 acggggactg gggacactgg acgtacaaga gacgggtccc tttgggactc aacgtatcgg
     6901 cccgtctgct ggcggaagcc tggctgatgg agccgtgcag ggcttcgctc gcatcatgta
     6961 aaccgaggaa ggcgaccatg tcatcctggt ccacgccatc gcccgagacg ccgatggcac
     7021 cgaccaggat gtcgccgcga taaatgggca cgctcccggg aaagatctgc agtccgttct
     7081 tcagtgctgc ggaggcggtg ctcgcggtga tgctgactgc ggccgagacc ggcggcacgc
     7141 tcaacccgac gcccgcacag ccgactggca cgtccggaac acccggcaca ccgatcgcat
     7201 agacgacgtg ctgcaggatc gcattgagcg atagatcgag ctgcagaccg gtcgagaaca
     7261 cgctccactg cccgatcggc ttgctgaagg gtccgttgcg tgtgccatcg ataccgtccg
     7321 ggaaaagcgg gcgggagagg ttgccgatgg cgcgattgct gtaggcgatc acgccgtcgg
     7381 cgagtgctct gttgtccccc aggaatgact gcgcggcgct cacgtagctg gccagaggca
     7441 cccggccgtt cgaggtcaaa ccacttgcgt tgatcgagac gtactgcgcg ggcggcaggg
     7501 aggaaaggaa tgccgctgcg gtgctcgagg agaacaatgc cgcagtcctg gccttctgta
     7561 aggcgacgtc agcgccgaac aaaggtgcat cccgcgaacg gatgaagccg agcggcgtgc
     7621 ccagtgtgtc aacgaccgtg atggacactc gagcggtgtc accgagcggc agcctgatct
     7681 ggccgcgcgc tttttccgct atcgcgaacg aggagcgcaa tacctgcagc acctcgtctc
     7741 gggtcagcgc attcgggcca tccgtgccgg ccttggcagg aaagcgcggc gtgttggtgg
     7801 catcgaccag aacgaaggca tcctgtccgg gaaagtcggc gccgccctcg gggcggactc
     7861 ccgaggaggg ctgtccgaag gccgtccccg tgtggatgtg gccgtcggag tagcccgtta
     7921 cggcaatcag actgcccacc gaaggcgaca gggaactgta cgcaggcgcc gagcccggat
     7981 ccgtcgacag cgcgctgtat tcgatgttgg tgtatcgcag cgcgagaccg cccacggtga
     8041 tctgatcagc ccgccggtcc gccggcgcac cgaagttgaa cgtggccgcc atggcaatcg
     8101 cttcgtcaac atcgac
//
LOCUS       DASRHN010000339        15511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1106826, whole genome shotgun sequence.
ACCESSION   DASRHN010000339 DASRHN010000000
VERSION     DASRHN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106826"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1032)
                     /gene="fghA"
                     /locus_tag="VFB37_13570"
     CDS             complement(166..1032)
                     /gene="fghA"
                     /locus_tag="VFB37_13570"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HZO23527.1"
                     /translation="MARTAAFDVISQHQCFGGVVGFYRHDSTSTASSMRFAVFSPPQA
                     RAGRVPVLYYLAGLTCTEETFMIKAGAQRLAAEFGLMLVAPDTSPRGLKLPGESDSWD
                     FGVAAGFYVDATQEPWAGHYRMYSYVTQELRSLIESEFAADGARTGIFGHSMGGHGAL
                     TLALRNPGLYKSISAFAPIAAPRQCPWGQKAFNGYLGPDQGQWSAYDATELVAGIRDA
                     GKRPTILIDQGQADQFLEQQLHPHLFEEASRRVGYPLTLRRHAGYDHSYYFMSSFMAD
                     HLRHHARSLGVR"
     gene            1224..1964
                     /locus_tag="VFB37_13575"
     CDS             1224..1964
                     /locus_tag="VFB37_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HZO23528.1"
                     /translation="MGQPRNAPRLRAVLLVTALVGALLTARTAEALTPGGDATLTSDY
                     IFRGISESAGDPALQLDLHVSSADGTFAGAFASTLRRIRHHGADDELQEYLGHHFDLS
                     PEWTTTLTLVNYSYLDSNLPFSDDYQELSAALSYLDLWTVSVTASPNVPRYAGEYRLG
                     RYTAYVTDASAQVPLVGRLFATAGLGHYTLNGPAGTSYVYGNAGLSFESGAWRIDAAY
                     YVVQSRVQYLFPYGEARNRFALTLSWHF"
     gene            2034..2675
                     /locus_tag="VFB37_13580"
     CDS             2034..2675
                     /locus_tag="VFB37_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZO23529.1"
                     /translation="MRGPSHTETQGPPGTGESGYRSVASAAREAALIARIAQGDRRAF
                     EELYNLYHRRLARFLTRLTRRYDIAEEVINDTFWIIWRKAGDFRGDSQPSTWILGIAY
                     RKARNAFRSSARLAAMENPEDPLQPPTSEEPMRAEELRDWLVRALAQLPVEQRLAVEL
                     CYELGYSCEEIGAIMGCPVNTVKTRLFHARAKLQKILPVLGGTAAPEERRGLK"
     gene            2672..3421
                     /locus_tag="VFB37_13585"
     CDS             2672..3421
                     /locus_tag="VFB37_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23530.1"
                     /translation="MMTLDERQEHQEIWLLLPWLASGRLSPTERERAEQHVHRCLPCQ
                     NEFSLQQRMCSAFAEPDRVVYAPGPSFRKLMERIDSEAQTDRRGARAAVQPHLSPRGI
                     AARLSHVSLWRPPGAAWAASFLALFALTGLLATAYRWSEPLYRTHTEVTAETPNVLHI
                     ALDRRLTIAEVEDLLRTQGARVVEGPGSTGIFGVSPSGLAPGTPAASVKQRLRDLARR
                     LHADPRVVWVQPLAQDDTPTLSPGAGAPPER"
     gene            3421..4701
                     /locus_tag="VFB37_13590"
     CDS             3421..4701
                     /locus_tag="VFB37_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZO23531.1"
                     /translation="MPSPAYRWPLAALCLLAIIPCASGQAPADFSAAGPLIVVTFANQ
                     PYRSAPRAGTTGHRYAGDRYGISLDAHERARRIAALYSLHEMASWPIKELAVHCVVYE
                     IPDSRSVKEVLSALTKDPRVSLAQPMQEFHTLTQSQPGDAYNDPLYGLQTNLAALNIA
                     AAHQRTEGEGVRIGLIDTGVDTAHPDLLERITGTHSFINSSVISASSFRHGTAMAGLI
                     GAVANNHVGMVGIAPRAQIEVFEACWQLKPDADEAACNTFTLAKALAAAIEAHLPLVN
                     LSIAGPPDPLLSALVQSGLQRGMIFVGAMTDAAAFPTNIDGVIGVGSSEHAQTRATLV
                     APATHVLTLRPQGQYDFVSGTSVATAEMTGVIALLLSANARLSVDSVVSVLKETTAAS
                     GEAPGLRSVDAGAAVSRLYTERGSGRMASARSAR"
     gene            4842..5885
                     /locus_tag="VFB37_13595"
     CDS             4842..5885
                     /locus_tag="VFB37_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO23532.1"
                     /translation="MRMKRLGNTGLVVSELCLGTMTFGTGEGMWRSIGKLEQPAVDSM
                     VRTAFEHGINFVDTADVYHAGRSEITTGVALKNLGLARDEFVLATKVHGRMGPGANQA
                     GLARSHILQAVDQSLQRLQLQHIDLYQIHGFDPLTPLEETLGALNDCVRAGKVRYIGL
                     CNLAAWQIAKSLGICARRGFERFQSVQAYYSIAGRDLEREIVPLAMEEQLGILPWSPL
                     AGGLLSGKFSRDGKGPEGSRRVAFDFPPVDKDRAFRVIEAMRPIAEAHRVSVAQIAIA
                     WLLHQPPVTSVIIGARTNEQLRDNLAACDLKLDPAQISTLNKLSALPAEYPGWMLERQ
                     AADRAALLNPSHS"
     gene            complement(5866..6642)
                     /locus_tag="VFB37_13600"
     CDS             complement(5866..6642)
                     /locus_tag="VFB37_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO23533.1"
                     /translation="MAAPEAVHASAAKGFARDAEAYARGRPEYPVALDPWLRTTLGLG
                     PARTVLDLGAGTGKLTRRLLATDAAVVAVEPVPEMLARLRESFPQVEARSGTAQAIPL
                     ADATVDAVTCGQSFHWFATATALREIHRVLRPGGHLGLVWNMRDESEAWSVALTRILT
                     RYEGDAPRFRSGAWRKLFPATGFGALREQRFAHRHSGPAEQVIVDRVLSVSFIAALEP
                     SERARVAAQLRELISSTPSLSASPTVHVAYQTLAVSCVRD"
     gene            complement(6645..8663)
                     /locus_tag="VFB37_13605"
     CDS             complement(6645..8663)
                     /locus_tag="VFB37_13605"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZO23534.1"
                     /translation="MVSEGELLWTPSAERVRRSQLTAFQNWLQSRHGLRFSSYEELWR
                     WSVRDLESFWQAIWDFFGTESSRPYECVLADRRMPGAKWFPGARLNYAQHALRHERAD
                     ADAILFLSERRPLSRMSWQELAAQVRTLATQLRRLGVQPGDRVVAYLPNVPEAIVAML
                     ATTAIGAIWASCGPDFGTRGVLDRYIQLAPKVLFCTDGYQYGGKPFDRKAEVRGIIEQ
                     LPSLQHVIYFPYLRAEDLEPLSSHTIFWDELLAGPPVAAQEFSFTQVPFDHPLWILFS
                     SGTTGLPKAICHGHGGIILEQQKHLAFNFDLPPGGRLFYITTTGWMMWNVLAGALLAN
                     LVPVLYDGHPAWPGMDVLWRLVDEAGLTLFGTSPAYVASLAKAGFVPREKFLLTSLET
                     VKLVGSPVTPECMDWLYRNVKRDLWVSSGSGGTDVCTGFVAGASILPVRAGEIQARSL
                     GAAVYAFDERGEKVVNQVGELVVTEPMPSMPVCFWNDPGGERYRDAYFREYPGVWRHG
                     DFFRLNDRGACFVLGRSDATLNRHGVRIGTAEIYRSLAALAEIDDALIVNLDLPHGKF
                     FMPLFVKLREGRVLDEGLRERIRATLRRDYSPRHVPDAIHEVPGIPLTLTGKKMEVPV
                     RRILMGVPPERAANRDAVANPQALDYFTRYAHSQTDYSLAAPSAASGS"
     gene            complement(8686..9891)
                     /gene="pcaF"
                     /locus_tag="VFB37_13610"
     CDS             complement(8686..9891)
                     /gene="pcaF"
                     /locus_tag="VFB37_13610"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HZO23535.1"
                     /translation="MADAFICDAARTPIGRYGGALASVRPDDLAAIPISALLERNPSL
                     ERSAVEDVLLGCANQAGEDNRNVARMAALLAGLPVEVPGATINRLCGSGLDAIANAAR
                     LIRTAEAELIIAGGVESMSRAPFVIPKAESAFSRNAEIYDTTIGWRFINPLLKQKYGV
                     DSMPETGENVAADYEISRQDQDRFALRSQQRTAAAYARGFFDAELVPVRIPQRKGAAI
                     IVSRDEHPRPETTLEQLAKLPTPFRANGTVTAGNASGVNDGACALVIASEAAARRHGL
                     TPRTRIVAAAAAGVAPRTMGMGPVPATIKALGLAKLDIRTMDVVELNEAFASQGLAVL
                     RELGLPDDAEHVNPNGGAIAIGHPLGMTGARLATTATYQLLSTRGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            10117..10614
                     /locus_tag="VFB37_13615"
     CDS             10117..10614
                     /locus_tag="VFB37_13615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO23536.1"
                     /translation="MLIRDAPEGDLPGLLDIYNEVIATSTAIYSSVPATLQDRRDWWC
                     KRLASGYPVLSAVDRSGVLGFASFGEFRAGPGYRFTVEHSVHVRAGCRGRGIGQALMA
                     ALFTRAQTLGKHVMVAGVDGSNSSSIRFHERLGFERVGHLRETGCKFGRWLDLVLLQR
                     LITDP"
     gene            10625..11272
                     /locus_tag="VFB37_13620"
     CDS             10625..11272
                     /locus_tag="VFB37_13620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZO23537.1"
                     /translation="MGLFPVDYTGTLRSMLPSLPPVTRALIFINILVFLLQHVPGLQV
                     DAYFALWPLGTQFAVWQLITYAFLHGSLAHIFFNMFALYMFGGALESFWGPRRFLLFY
                     FVSVLAAAATQLTVQVLAQSGEPVIGASGGIFGLLLAFAWYFPRQRILLLFPPIPMPA
                     WLFVTLYGALELVLGVSGAQSSVAHFAHLGGMLGGALMILIWRAQDAARRSRRPF"
     gene            11269..12225
                     /locus_tag="VFB37_13625"
     CDS             11269..12225
                     /locus_tag="VFB37_13625"
                     /inference="COORDINATES: protein motif:HMM:NF006094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZO23538.1"
                     /translation="MSIGAAVVDDLEPPCIELAYTRIGSHIRRTPVLEVDAQDFGLAP
                     RVLGLKLELLQHGGSFKARGAFANLLMRELPAAGVVAASGGNHGVAVALAARKLGVRA
                     KIFVPSVAAAAKQQQIRRLGAELVIAGERYADALEASQHWAERSGALQVHAYDQRETL
                     LGQGTLGLELERQNALPDTLLVAVGGGGLIGGLAAWFRGRIKLVSVEPARAPTLAKAL
                     QAGRPVDAEAGGIAADSLAPRRVGELMFPLAQKFVGAAVLVEDDAIREAQRLLWREAR
                     IACEPGGAAAFAALLSGRYVPRPEERVAVILCGGNTGAVSFD"
     gene            12548..13471
                     /locus_tag="VFB37_13630"
     CDS             12548..13471
                     /locus_tag="VFB37_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO23539.1"
                     /translation="MIKTEHLSKRYDALVAVDDVTFQVGPGEVLGFLGPNGAGKTTTM
                     RMLAGFVTPSAGRASICGHDIESDPLAAKASLGYLPEGAPTYGEMTVRSFLDFIADLR
                     QLEGERRRARLDYVIERLQLSSVLEQTIETLSKGFRRRVGLAQAIVHDPPVLVLDEPT
                     DGLDPNQKHEVRTLINEMARDKIIVISTHILEEVNAVCTRAIIIARGRIVADDTPVAL
                     AGKSRYHNAVSLQLERADQLEAAREAVRSLPSVAEIEVSERDARITALPRAGATLLPA
                     LSELASRQGLKLKELHLESGRLDEVFRTITA"
     gene            13505..14239
                     /locus_tag="VFB37_13635"
     CDS             13505..14239
                     /locus_tag="VFB37_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO23540.1"
                     /translation="MRNVGIIMRRELASYFATPLAYVFILIFLVLANFFTFSLGGFFE
                     RDQADLQPFFTYHPWLYLFLVPALSMRLWAEERKSGSIELLMTQPVTLWEAVLGKFLA
                     AWLFTALALALTFPLWITVNYLGKPDNGTILAAYLGSLLLAGGFLAIGSCTSALTRNQ
                     VIAFILGVVVCFAFVLAGFPPLLNLLTGWVPQVIVDAIASLSFLTHFESIAKGVIDVR
                     DLLYFAMLIGFFLLATSIALDLRKAN"
     gene            14255..>15511
                     /locus_tag="VFB37_13640"
     CDS             14255..>15511
                     /locus_tag="VFB37_13640"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HZO23541.1"
                     /translation="MTNRSTLGGGALLALALLFIGLTILFNHALLGWRLDLTQNHLYT
                     TAPGTDRILKSIREPINLYFFYSDKTASQIWEIKTYAVRVRELLQELARRSGGKVKLH
                     IIDPQPFSEDEDRAAELGVRGTPIGQSGAQLYFGLAGTNSTDGHQAIEFFDPHKEAFL
                     EYDVVKLIYELANPKKPVVAWLSTLPMTSGFDPQSGQMREPWAVYGEAQQLFDLRPLE
                     PNATKIDPDVNVLVLVHPKNLTPATQFAIDQFALRGGHVVAFVDPLAEADRSGADPQN
                     PMAAMTADRSSQLGNLLKAWGVQFNPSQVVADRARALSVSMRRGEEPVPHVGILGLTS
                     ADFATSDVTLAGLSNVNLATAGHLEPIKGSRTRFEPLLRSSNDAELLPVQRFAMLLDP
                     ATLRDGFKATGERYTIAARVTGDVKSA"
BASE COUNT         2647 a         5159 c         4948 g         2757 t
ORIGIN      
        1 ggggctcgaa gctgaccgcc tcggtcatcg gacttaccac ctttggccgg gcagcgccga
       61 ccctgcgaag gatgcgcaag aagtatacgt ccgcccggct cggcggcagc gatccacctg
      121 cacggccaac gatccgggtg caacagccct tcggccaccg cgagctcagc gcacgccgag
      181 gctcctggcg tgatgccgca aatgatccgc catgaagctc gacatgaagt agtaactgtg
      241 gtcgtaaccc gcgtggcgtc gcagcgtcaa cgggtagccg acccggcgac tcgcctcctc
      301 gaacaggtgt ggatgcaact gctgctccag gaactgatcg gcctggccct gatcgatcag
      361 gattgtcggt cgcttgccgg catcccgaat cccggcgacg agctcggtcg catcgtaagc
      421 actccactgg ccctggtccg ggcccagata gccgttgaag gccttctgac cccaggggca
      481 ttgcctgggc gcggcgatcg gtgcgaaggc ggagatcgac ttgtagagcc caggattgcg
      541 cagtgcgagc gtgagggcgc cgtggccgcc catggaatgc ccaaaaatgc cggtccgtgc
      601 tccgtccgcg gcgaactcgc tctcgatcag tgatcgcaac tcctgggtga cgtagctgta
      661 catccggtag tgtccggccc acggctcctg ggtcgcgtcc acgtagaagc ccgccgccac
      721 gccaaagtcc caagagtcgc tctcgcccgg tagcttgagg ccgcgcgggc tggtgtcggg
      781 cgcgacgagc atcaggccga actctgccgc gagacgctga gcgcccgcct tgatcatgaa
      841 ggtctcttcg gtgcaggtca ggccggccag gtaatacaac accggcaccc gccccgcccg
      901 cgcctggggc ggcgagaaca ccgcgaatcg catggaagaa gcggtactgg tggagtcgtg
      961 gcggtaaaaa cccacgacgc cgccgaaaca ttggtgttgg ctgatgacat caaatgctgc
     1021 tgtgcgcgcc atgggattac cttcgatcgg tcgtgactcg agtgatgccc ggacattatg
     1081 gccgggaacg ctcggatgcg tgaggcgtcc gtccggggcc gcgtcaggct gctccggagg
     1141 tggggcagat ggaccggccg ccgttccccg aatggcacta aactggcggt gcgccgagga
     1201 acgcgcctgt cctgcgaggc tcagtgggtc aacctagaaa cgctccgcga ctgcgcgccg
     1261 tcttgctcgt gacggctctg gtcggggccc ttctcaccgc ccgcaccgct gaggccctca
     1321 caccaggggg cgatgcaacc ctgacctcgg actacatctt ccgcggcata tccgagagcg
     1381 ccggagaccc ggcgctgcag ctggatctgc acgtcagcag cgcggacggg acattcgccg
     1441 gcgcttttgc ctcgacgctg cgcaggatca ggcatcacgg cgccgacgat gagctgcagg
     1501 aatacctggg ccaccacttc gatctttcac cggagtggac gacgactctc acgctcgtga
     1561 attactcgta cctcgacagc aaccttccgt tctccgacga ctaccaggag ctctcggcgg
     1621 ccctgtccta tctggacctg tggaccgtct cggtgacggc gagccctaac gtgccgcgat
     1681 atgcgggtga gtatcggctc gggcgttata cggcctatgt gactgatgcc tcggcgcaag
     1741 tgccgctcgt ggggcggctg tttgcgacag cgggcctcgg gcattacacg ctgaatggac
     1801 cggccgggac cagttatgtc tacggaaacg ccgggctctc gttcgagtcc ggagcctggc
     1861 ggatcgatgc ggcttattat gtggtccagt cgcgggtcca gtacctgttc ccctacgggg
     1921 aggcccggaa ccgctttgcg ctgaccctga gctggcattt ttgaagccgc cggcgagcgg
     1981 tatattgaac cccagtccgg ccgcaggaca cttaccctga aagggtacga ggcgtgagag
     2041 gtccatcgca caccgaaacg cagggcccac cgggcaccgg tgagtcaggc tatcgcagtg
     2101 tcgcttccgc ggcccgcgag gccgctctca tcgcacgcat cgctcagggc gaccgtcgtg
     2161 cattcgagga gctgtacaac ctttatcacc gccgccttgc ccggttcctg acgcgtctca
     2221 cgcgtcgtta tgacatcgcg gaagaggtca tcaacgacac cttctggatc atctggcgca
     2281 aggccggcga cttccggggc gattcccagc cctcgacctg gattctcggc atcgcctatc
     2341 gaaaagcgag aaacgcgttc cggtcctcgg cacggctggc cgccatggag aacccggagg
     2401 accccctgca gccacctacg agtgaagagc caatgcgggc cgaggagctg cgcgactggc
     2461 tcgtgcgcgc cctggcacaa ctgccggtgg agcagcgcct ggcggttgag ctctgctatg
     2521 agctcggtta ttcgtgcgag gagatcgggg caatcatggg ctgtccggtg aacaccgtga
     2581 agactcgcct gttccacgcg cgcgccaagc tgcagaagat tctgcccgtg ctcggcggaa
     2641 cggccgcgcc cgaagagaga cgaggcctca aatgatgacg ctcgacgaac gccaagagca
     2701 tcaggagata tggctgctat tgccctggct cgccagcggg cgactgagcc cgaccgagcg
     2761 cgagcgggcg gagcagcacg tgcaccgctg cctcccctgc cagaatgagt tctcgctgca
     2821 gcagcgcatg tgcagcgcct ttgccgagcc ggatcgcgtg gtgtacgcac cgggtccctc
     2881 cttccgcaag ttgatggaac gcatcgactc ggaggctcag actgatcggc gtggcgcgcg
     2941 cgcagctgtt cagccgcatc tgtctccccg cgggatcgcg gccagactga gccatgtctc
     3001 gctgtggcgc ccgcctggag cggcctgggc cgccagcttt ctggcgctat ttgcccttac
     3061 tggtttgctg gcgaccgcgt atcgctggtc cgagcctctt tatcggaccc acaccgaggt
     3121 cacggccgaa actcccaacg tgctgcatat cgcgctcgac cgccgactga ccatcgccga
     3181 agtcgaggac ctgctgcgca cacagggcgc gcgggtggtc gaaggaccgg gcagcacggg
     3241 catcttcggt gtctcgccga gcggactggc ccctggcact ccggctgcga gcgtgaagca
     3301 acggttgcgg gacctcgcca ggcgtctgca tgccgatccg cgggtcgtgt gggtgcagcc
     3361 gctggcgcag gacgacacgc caaccctgag ccctggagct ggcgcgcctc cggagcggtg
     3421 atgccctcgc ctgcataccg ctggcccctc gccgcgctct gtctgctggc gatcataccg
     3481 tgcgcgtccg ggcaggcgcc cgcggacttc agtgccgccg ggccactcat cgtggtcacc
     3541 ttcgccaacc agccttaccg atcagcgccg cgcgcgggga ccaccggcca ccgctatgca
     3601 ggtgatcgct acggcatctc gttggacgct cacgagcggg cgaggcgcat cgccgccctg
     3661 tactcgttgc acgagatggc gagctggccc atcaaagagc tggccgtaca ctgtgtcgtc
     3721 tacgagattc cggattcgcg ctcggtgaag gaggtcctgt ccgcgctcac gaaggacccg
     3781 cgtgtcagcc tcgctcaacc gatgcaggaa ttccatacgc tcacgcagtc gcaaccgggc
     3841 gacgcgtaca acgacccgct ttatgggctg caaaccaacc tcgcggcgct gaacatcgcc
     3901 gccgcgcacc agcgcaccga aggcgaggga gtgcggatag gcctgatcga cacgggagtg
     3961 gatacggccc atccggatct gctcgagcgc atcacgggca cacactcatt catcaactca
     4021 tcggtcatat ccgcgagctc gttccggcat ggcacggcca tggccggtct catcggggca
     4081 gtcgccaaca accatgtcgg catggtaggc atcgcgcctc gcgctcagat tgaagtgttc
     4141 gaggcgtgct ggcagctcaa gccggatgcg gatgaggcgg cctgcaacac cttcactctg
     4201 gccaaggcac tcgctgctgc catcgaggcg cacctgccgc tggtgaatct cagtatcgca
     4261 ggaccgcccg acccgctgct cagtgcgctg gtgcagagcg gcttgcagcg cggcatgatc
     4321 ttcgtcggcg cgatgacgga tgcggcggcc ttcccaacca acatcgatgg cgtgatcgga
     4381 gttggcagct ccgagcatgc gcagacccgc gccaccctgg ttgcacccgc gacgcacgtc
     4441 ctgacgctcc gtccgcaggg gcagtacgac tttgtctcgg gtacctccgt ggcgaccgcc
     4501 gaaatgacgg gcgtcatcgc attgctgctc agcgcaaatg cccggctgag cgttgactcc
     4561 gtcgtctcgg tgttgaagga aaccacggct gcttccggtg aggcgcccgg gctgcgaagc
     4621 gtagatgccg gcgcagccgt atcccgcctg tataccgagc gtggcagcgg ccgaatggcc
     4681 tcagcgcgtt ccgcgcgcta gcatgacttt ttcgaagccc tgctagagat tccctgactt
     4741 ccgcgcaaat cggggcagct gccgcggccg caggctcgtt tgttgcgggc cgcgactgct
     4801 catgctgcgg gctactctgc agcgccacga ggaaagcgca catgcggatg aaaagacttg
     4861 gcaatacggg actcgtcgta tccgagcttt gcctcggcac catgacgttc ggcacgggcg
     4921 aaggcatgtg gcgctccatc ggaaaactcg agcaacccgc ggtcgattcc atggtgcgca
     4981 cggctttcga gcatggcatc aattttgtgg ataccgcgga tgtctaccat gcaggccgct
     5041 ccgaaatcac caccggggta gccctgaaga atctcggtct cgcccgcgat gaatttgttc
     5101 tggcgaccaa ggtgcacggt cgtatgggtc cgggagcgaa tcaggcgggc ctcgcgcgca
     5161 gccacatcct gcaggcggtg gaccagagcc tgcagcgcct gcagctgcag cacatcgatc
     5221 tctaccagat ccatggcttc gatccgctga cgccgctgga ggaaactctg ggcgcgctga
     5281 atgactgcgt tcgcgccggc aaggtgcgct atatcggcct gtgcaacctg gccgcctggc
     5341 agattgcgaa atcgctgggt atctgcgcgc gccgcggatt cgagcgtttc cagagtgtcc
     5401 aggcctatta cagcatcgcc ggacgggatc tggagcggga gatcgtgccg ctcgccatgg
     5461 aggaacagct gggcattctg ccgtggagtc ccctcgcggg cgggctgctc agcggcaagt
     5521 tctcgcgcga cggcaagggg cccgagggca gccgccgggt ggctttcgac tttccgccag
     5581 tggacaagga ccgcgctttc cgcgtgatcg aggccatgcg cccgatcgcc gaagcccatc
     5641 gcgtgtcggt cgcacagata gcgatcgcct ggctgctgca ccagccgccg gtcacttccg
     5701 tgatcatcgg tgcccgcacc aacgagcagc tccgggacaa tctggccgcc tgtgacctga
     5761 agctcgaccc ggcgcaaatc tccaccctca acaagctctc agccctcccg gccgagtacc
     5821 ccggatggat gctggagcga caagccgccg atcgcgccgc cctgctcaat ccctcacaca
     5881 gctgacggcc agagtctgat acgcaacgtg gaccgtcggg cttgcgctca gcgagggcgt
     5941 gctcgagatc aactcacgca gctgcgcggc gacgcgcgct cgctccgagg gctcgagcgc
     6001 cgcaatgaag ctcaccgaga gaacacgatc cacgatcacc tgctccgccg gcccactgtg
     6061 ccggtgtgca aaacgctgct cccgcaaggc accgaagccg gttgcaggga acagtttccg
     6121 ccaagcgcca ctgcgaaacc gcggcgcatc gccttcgtag cgagtcagga tccgggtcag
     6181 ggccacactc caggcctcgg actcatcgcg catattccag accagtccca ggtggccgcc
     6241 tggccgcagc acccgatgaa tctcccgcag cgcggtcgcg gttgcgaacc agtgaaagga
     6301 ctgcccgcag gtcacggcat cgaccgtggc atcggccagc ggaatcgcct gggctgtccc
     6361 gctgcgcgcc tcgacctggg gaaagctctc gcgcagtcga gccaacatct cgggcacggg
     6421 ctctaccgcc acaaccgccg catcggtggc aagcagccgt cgcgtcagct tgccggtgcc
     6481 ggctccgaga tccaacacag tgcgcgcagg tccgagcccc agcgtggtgc gcagccatgg
     6541 atcgagcgca accggatact ccggtctgcc gcgtgcgtag gcttccgcgt cgcgggcaaa
     6601 gcctttcgcc gcggacgcat gcacggcttc tggagcagcc atgatcagga gccactcgcc
     6661 gccgaaggag ctgcgagcga gtaatccgtc tggctgtggg cgtagcgggt gaaatagtcg
     6721 agcgcctgcg ggttggcgac ggcgtcgcgg ttggcggccc gctccggcgg cacgcccatc
     6781 aggatccgcc gtaccggcac ctccatcttc ttgccggtca gggtgagcgg aatgcctggg
     6841 acctcgtgaa tcgcatccgg cacatgccgg ggagaataat cccggcgcaa ggtcgcacgg
     6901 atccgctcgc gcagtccctc atcgagcacc cgcccctcgc ggagctttac gaacagtggc
     6961 atgaagaact ttccgtgggg caagtccagg ttgacgatga gcgcatcgtc gatctcggca
     7021 agcgcggcga gtgagcggta gatctccgcc gttccgatcc gtacaccgtg gcgattgagc
     7081 gtcgcatccg agcgacccaa cacgaagcag gccccgcgat cgttcaggcg aaagaaatcg
     7141 ccgtgccgcc agacacccgg atactccctg aagtaggcgt cgcgatagcg ttcgcctccc
     7201 gggtcgttcc agaagcaaac aggcatggaa ggcatcggtt cggtgacaac gagctccccc
     7261 acctgattga cgaccttctc gccgcgctca tcgaaggcgt agacggctgc cccgagcgat
     7321 cgcgcctgaa tctccccggc acggaccggc agaatggagg cgccggccac gaatcccgta
     7381 cagacatcgg tgccgccgct gcccgaggag acccacagat cgcgcttcac gttgcggtaa
     7441 agccaatcca tgcactccgg cgtgacgggt gagcccacga gcttcaccgt ctcgaggctc
     7501 gtcagaagaa atttttcccg cgggacgaag cccgctttcg cgagcgaggc gacataggcg
     7561 gggcttgtgc cgaagagcgt caaccccgcc tcgtccacca gtctccagag cacatccata
     7621 cccggccagg ccggatgccc gtcgtagagg accgggacga ggttggccag cagcgcgccc
     7681 gccagcacgt tccacatcat ccagccggtc gtggtgatgt agaacaaccg cccgcccggg
     7741 ggcagatcga aattgaacgc gagatgcttc tgctgctcga gaatgatgcc gccatggcca
     7801 tggcagattg ccttgggcag gcccgtcgtc cccgaggaga aaagaatcca caacggatga
     7861 tcgaacggaa cctgcgtgaa gctgaattcc tgcgcggcta ccggtggacc tgcgagcagc
     7921 tcgtcccaga agatcgtatg gctcgagagc ggttcaaggt cctcggcacg cagatacgga
     7981 aagtagatga cgtgctgcag cgaagggagc tgctcgatga tgccgcgcac ctcggccttg
     8041 cggtcgaagg gcttgccgcc gtactgatag ccatcggtgc agaacagcac tttgggcgcg
     8101 agctggatgt agcggtcgag cacgccgcgc gtgccgaagt ccggcccgca gctcgcccag
     8161 atcgcgccaa ttgccgtggt ggccagcatg gccacgatcg cttcgggcac gttgggcagg
     8221 tacgccacga cacggtcccc gggctgcacg cccagcctgc gaagttgggt cgccagcgtg
     8281 cgcacctgcg cagcgagctc ctgccatgac atccgtgaca ggggccggcg ctcgctcaga
     8341 aacaggatgg catcggcgtc ggctcgttcg tggcgcaagg cgtgctgggc gtagttcagc
     8401 ctcgcgccgg gaaaccattt cgcccccggc atgcgccggt cagccagcac gcactcgtaa
     8461 ggacgcgagg actcggtgcc gaagaaatcc cagatcgcct gccagaagct ttccaggtcg
     8521 cgtacggacc agcgccacag ctcctcgtaa ctgctgaatc gcaggccgtg cctgctctgc
     8581 agccagttct ggaaggcggt gagctgggat ctgcggacac gctcagcgct gggtgtccac
     8641 aacagctcgc cctcgctcac catgcgtagc gggccccgcg catcgctaga cccgctccag
     8701 gatcatcgcg attccttgcc cgacgccgat gcacatcgtg cacagcgcat agcgtccccg
     8761 ggtcgagagc agttgatagg tcgcggtcgt cgccaggcgc gctccggtca tgcccagtgg
     8821 atgtccgatc gcaatcgccc ctccgttggg attgacatgc tcggcatcat cgggcagccc
     8881 gagctcacgc aacacggcaa gtccctggga ggcgaacgcc tcgttgagct ccacgacatc
     8941 catggtccgg atgtcgagct tggcgagccc cagcgccttg atggtagcgg gcaccggacc
     9001 catgcccatc gtacgcggag cgacgcctgc ggcagccgct gccacgatgc gtgtgcgtgg
     9061 agtcaatccg tggcgcctcg cagcggcttc agatgcaatc accagagcac acgccccgtc
     9121 gttgacgccc gacgcattgc cggccgtcac ggtgccattg gcgcggaacg gagtcggcaa
     9181 ctttgcgagc tgctcgagcg tggtttccgg tctcggatgc tcatcgcggc tgacgatgat
     9241 cgcagcgccc ttgcgctgcg ggatccgcac gggaacgagc tcggcgtcga agaagccgcg
     9301 ggcataggct gcagcggtcc gctgctgact gcgcagtgca aacctgtcct gatcctgacg
     9361 cgatatctcg tagtcggccg cgacgttttc gcccgtttcc ggcatcgaat ccacgccata
     9421 tttctgcttc agaagcggat tgatgaagcg ccagccgatc gtggtgtcgt agatctctgc
     9481 attacggctg aaggccgact ccgccttggg aatgacgaat ggcgctcgcg acatcgattc
     9541 gacgccgcct gcgatgatga gctcagcctc agcggtgcgg atgaggcgcg ccgcattggc
     9601 gatggcgtcc agccccgatc cgcacaggcg gttgatggtg gctcccggca cctccacggg
     9661 caggccggcc agaagcgcgg ccatcctggc cacattgcga ttgtcctccc cggcctgatt
     9721 cgcgcagccc agcaggacgt cttcaacggc cgagcgctcg agcgaagggt tccgctccag
     9781 aagcgccgag attggtatcg ccgcaagatc atccggacga acgctggcga gggccccgcc
     9841 atagcgcccg ataggcgtgc gtgcggcatc acagataaag gcatcagcca tcgatcaggc
     9901 ctcggagcgg tcacaccggg gcatccatga tagcgccgcc ttggagccgg tggaccgagt
     9961 acccgcgtgc gcatttcccg gccagcgtcc acgcgctcag gccgagtgca aaggctgctc
    10021 tcagcgcact catccggggc atgcctcgcc gacagaccgg cgcaggccgc atcgccaggc
    10081 ggctgaggct ggcacgaggg ttgcacccca tggcccatgc tgatccgcga tgcgcctgag
    10141 ggcgatttac cgggacttct cgacatctac aacgaggtca tcgcaacctc gaccgccatc
    10201 tacagctcgg tgcccgcaac gctgcaagac cggcgtgact ggtggtgcaa acgtctcgct
    10261 tcgggttacc cggtgctctc ggcggtcgat cgttcaggtg tcctgggatt cgcctcattc
    10321 ggagagttcc gcgccggacc cggatatcgc ttcacggtgg agcattccgt gcacgtgcga
    10381 gcgggctgtc gtggtcgggg tatcggacag gcgctcatgg ccgcgctgtt cactcgcgcc
    10441 cagactctgg gcaagcatgt catggtggcg ggcgtggacg gctccaattc ctcctcgatc
    10501 cgcttccacg aacgcctggg ctttgagcgc gtgggacatc tgcgcgagac cggatgcaag
    10561 ttcggacgct ggctcgacct cgttctcctg cagcggttga tcaccgaccc ctagccggcg
    10621 acacgtgggc cttttccccg tggactacac gggtacactg cgctcaatgc tcccgtcgct
    10681 gccaccggtc acccgggccc tcatcttcat caacatcctc gtctttctgc tgcagcacgt
    10741 acccggtctg caggtggacg cctacttcgc attgtggccg ctcggaacgc agttcgccgt
    10801 gtggcagctc attacctatg ccttcctgca tggcagcctt gcgcatattt ttttcaacat
    10861 gttcgcgctt tacatgtttg gtggcgcttt ggaaagcttc tggggcccgc gccgcttcct
    10921 gctgttctat ttcgtgagcg tgctcgcggc agcggccacc cagctgaccg tgcaggtcct
    10981 ggcacagagc ggcgagcccg tgatcggcgc ctccggtggc atcttcgggt tgctgctggc
    11041 tttcgcctgg tatttcccgc ggcagcggat cctgctgctg tttccgccca ttcccatgcc
    11101 cgcgtggctc ttcgtgacgc tctatggagc gttggagcta gtgttgggag tcagcggggc
    11161 ccagtcgagc gtggcgcact tcgctcatct cggcggcatg ctgggcggag cgctcatgat
    11221 cctgatctgg cgggcgcagg atgccgcgcg cagatcccgc aggccctttt gagcatcgga
    11281 gccgccgtgg tggatgatct cgagcccccc tgcatcgagc tcgcgtacac acgcatcggc
    11341 tcgcacatcc gccgcacgcc ggtgctggaa gtcgatgcac aggatttcgg gctggccccg
    11401 cgcgtgttgg gactgaagct cgagctgttg cagcacggcg gctccttcaa ggcgcgcggc
    11461 gccttcgcaa acctgctgat gcgcgagctg ccagcggccg gtgtcgtagc cgcttcggga
    11521 ggtaatcacg gcgtcgccgt tgctttggcg gctcgcaagc tcggcgtgcg cgcgaagatc
    11581 ttcgttccga gtgtcgcggc agccgccaag caacaacaga tccgacgtct gggagcggag
    11641 ctggtcatcg ccggagaacg ctacgcggat gcactggaag cgagccaaca ctgggctgaa
    11701 cgctccggcg cgctgcaggt gcacgcttat gaccagaggg aaacgcttct cggacagggc
    11761 acgctcgggc tggagctcga acgccagaac gctttgcctg acaccttgct ggtcgcggtc
    11821 ggtggtggcg gcttgatcgg gggtctggcg gcgtggtttc gcgggcgcat caagctcgtt
    11881 tccgtcgagc ctgctcgggc gccgacactc gcgaaagcgc tgcaggccgg ccggccggtg
    11941 gatgcggaag caggcggcat cgccgcggac tccctggcgc cccgccgcgt cggcgagctg
    12001 atgtttcccc tcgcacagaa attcgtgggc gcggctgttc tggtcgagga cgatgcgatc
    12061 cgggaagcgc agcggctgct atggcgtgag gcgcgcattg cctgcgagcc cggcggcgcc
    12121 gccgcttttg cggcgctgct cagcggccgc tatgtgccgc ggcccgagga gcgtgtcgcg
    12181 gtcattctct gcgggggcaa tacgggagcg gtcagttttg attgatggac tgcggcaacc
    12241 gcgagattcc tgcacagcat ctcagatgta tatcgtaagt atctgatatt atagaatttc
    12301 cgctgcaaac acggaacggg tttggcccgg gtagggacgg cggtgcgagg gctcgcgaca
    12361 agagccggac ggttgcgata tcgccctccg ctggcttgaa tttcacgccg cgtcccgttc
    12421 acaatcgcgc ccccgccagc tgcggtgaac aataaacgta tcggcgcggc gagcaactcg
    12481 gatgccttgc gcctcacgtg tgcggcggaa cttttcggac tgggctttct ctctactgcg
    12541 ccatcgtatg atcaagaccg aacacctcag taagcgttac gacgcgctcg tggccgtaga
    12601 cgatgtgacc ttccaggttg gaccgggaga ggtgctcggc ttcctcggtc cgaatggcgc
    12661 cggcaagacg acgacgatgc ggatgttggc cgggttcgtc accccgagtg ctggccgtgc
    12721 cagcatctgt ggccatgaca tcgagtcgga tccgctggct gcgaaggcga gcctcggcta
    12781 cctgcctgaa ggcgcgccca cctatggaga gatgaccgtc cgcagctttc tcgatttcat
    12841 cgccgacctg cggcaactcg agggcgagcg gcgccgagcg cggctcgact acgtcatcga
    12901 gcggttgcag ctctcctccg tgctcgaaca gaccatcgag accctgtcca aaggctttcg
    12961 ccgccgggtc ggtcttgctc aggccatcgt gcacgatccg ccggtactgg tgctggatga
    13021 gcccaccgat ggtctcgatc cgaaccagaa gcacgaggtg cgcacgctca tcaacgagat
    13081 ggcgcgcgat aaaatcatcg tgatttccac tcatattctc gaagaggtga atgcggtgtg
    13141 cacgcgcgcg atcatcatcg cccgcggtcg catcgtcgcg gatgacacgc cagtcgccct
    13201 ggcgggcaag tcccgctatc acaacgcggt gtcgctgcag ctcgagaggg ccgaccagct
    13261 cgaggctgca cgcgaggcgg tccgcagcct accctcggtt gccgagatcg aagtgagcga
    13321 gcgggatgcg cgcatcacgg cgctgccgcg cgccggggca accctcctgc ctgcgctctc
    13381 ggagctcgcc agccgtcagg gcttgaagct caaggagctg catctggagt cgggccgcct
    13441 cgacgaggta ttccggacca tcaccgccta gagccgacca gggccagcag accgaaagga
    13501 caccatgcgt aacgtgggca tcatcatgcg ccgcgagctt gcgagttatt tcgcaacgcc
    13561 gcttgcgtat gttttcatcc tgatcttcct ggtgctggcc aacttcttca ccttctccct
    13621 cggcggcttc ttcgagcgcg accaggccga tctgcagccc ttcttcacct accacccctg
    13681 gctgtacctg ttcctcgtgc ccgcgctctc catgcggctg tgggccgagg agcgcaagtc
    13741 cggcagcatc gagctgctga tgactcagcc ggtgacgctg tgggaggcgg tgctcggcaa
    13801 gttcctggcc gcctggctgt tcaccgctct ggccctcgcg ctcacctttc cgctgtggat
    13861 caccgtcaac tatctcggca agcccgataa cggcaccatc ctggccgcct acctcggcag
    13921 cctgctgctg gcaggcgggt tcctcgcaat cggctcgtgc acctccgcgc tcacgcgcaa
    13981 tcaggtcatc gccttcatcc tcggcgtggt ggtgtgcttc gcattcgtgc tcgccggctt
    14041 cccgccgctg ctcaatctgc tgaccgggtg ggtgccgcag gtgatcgtgg atgcgatcgc
    14101 ctcactctcc ttcctgactc acttcgagtc gattgccaag ggggtgatcg acgtgcgcga
    14161 cctgctgtat ttcgcgatgc tgatcggatt cttcctgctc gcgacctcca tcgcgctcga
    14221 cctgcgcaaa gcgaactagc ggagctcccg ggacatgacg aatcgctcaa cactgggtgg
    14281 cggcgcactg ctggcgctcg ccctgctgtt catcggcctg accatcctgt tcaatcacgc
    14341 actgctcggc tggcggctcg acctcacgca gaatcacctc tacaccaccg ctcccggcac
    14401 ggaccgcatc ctcaagagca ttcgcgagcc gatcaatctc tatttcttct actccgacaa
    14461 gaccgcctcg cagatctggg agatcaagac ctacgcggtt cgcgtgcggg agctgctgca
    14521 ggagctggcc cgtcgctcgg gcggcaaggt aaagctgcac atcatcgatc cgcagccctt
    14581 ctccgaggac gaggaccggg ccgccgagct cggcgtgcgc ggcacgccga tcgggcagtc
    14641 cggcgcccag ctgtatttcg ggctcgccgg aaccaactcc accgacggac accaggcgat
    14701 cgagttcttc gatccgcaca aggaggcatt tctcgagtac gacgtggtga agctgatcta
    14761 cgagctcgcc aatcccaaga agccggtggt cgcgtggctt tccaccttgc cgatgacctc
    14821 gggcttcgat ccgcagagcg gccagatgcg cgagccctgg gcggtctacg gcgaagcgca
    14881 gcagctcttc gatctgcgcc ccctcgagcc gaacgccacc aagatcgacc ccgatgtcaa
    14941 cgtgctcgtg ctggtccatc cgaaaaatct gaccccagca acgcaattcg ccatcgacca
    15001 gtttgcgctg cgtggcgggc atgtcgtggc gtttgtcgat cctctcgccg aggccgaccg
    15061 ctccggcgcc gatccgcaga acccgatggc cgcgatgacc gccgaccgct cctcgcaact
    15121 gggcaatctg ctcaaggcgt ggggcgtgca gttcaatccg agtcaggtgg tggccgaccg
    15181 ggcccgggcg ctgtcggtct ccatgcgccg cggcgaggag ccggtgccgc atgtgggcat
    15241 tctcggcttg accagcgcgg acttcgccac cagcgatgtc accctggcag gcctctcgaa
    15301 cgtgaacctt gcaaccgccg ggcatctcga gccgatcaag ggatcccgga cccgcttcga
    15361 gcccctcctg cgctcgagca acgacgcgga gctgctgccg gtgcagcgct ttgccatgct
    15421 gctcgacccc gcgacgctgc gcgatgggtt caaggccacc ggggagcgct atacgattgc
    15481 cgcgcgtgtc accggcgatg tgaaatccgc t
//
LOCUS       DASRHN010000340         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_945819, whole genome shotgun sequence.
ACCESSION   DASRHN010000340 DASRHN010000000
VERSION     DASRHN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945819"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFB37_13645"
     CDS             <1..298
                     /locus_tag="VFB37_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HZO23542.1"
                     /translation="AAMEIMLNSPLIQDLIFKGEVAKIKEVMSRSTRLGMKTFDQSLF
                     ELYETGFISYEDALRNADSKNELRLRIKLESKRETKTIDDGALQIVEEEQGRSL"
     gene            314..1492
                     /locus_tag="VFB37_13650"
     CDS             314..1492
                     /locus_tag="VFB37_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZO23543.1"
                     /translation="MAANTTPAKEPADSKTTPAAREASLATTGTYAVASNGSHPTLNT
                     KPLFKLMVEKKASDLFFTSNAPIKIKIEGQILPVNKQVLTPETVRQTAFALMTAEQRE
                     EFIRQFELDFAISEPGLGRFRVNVFMQRGFPAMVLRYITADMPRLESLGLPEIMTDLV
                     LLKRGLILMVGATGSGKSTSLAAMINYRNENASDHIVTIEDPIEFLHTNKRSLVNQRE
                     VGIDTKSYSRALRGVVRAAPDVILIGEIRDRESMEAAIHLAGTGHLVLATLHANNCAE
                     SLDRIINMFPREQHNQIFLDMSQYLRAILAQRLVMGRDGKRLAAVEVMINTPHISELV
                     KKGDVIGIKEAITTSGERGIQSFDVSLRDLHKQGRIDLEEALASADSRANLEAKINFG
                     "
     gene            complement(1496..2698)
                     /locus_tag="VFB37_13655"
     CDS             complement(1496..2698)
                     /locus_tag="VFB37_13655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO23544.1"
                     /translation="MRFVLFYHSLVSDWNHPSAHMLRGVASELLASGHAVRIFEPSDG
                     WSLHNLREQGGDGVVKAFHSTYPTLRSSFYDPLTLDLDGALADADVVIAHEWNEPGFL
                     RRLGEHRARSRAYRLLFHDSPHSGARMSLEVRTQALQAFDGVLAASDALRRTYREQDG
                     PNRWTWRDAVDVRVFRPSPGEPALSRDLIWVGSWDVGERLGELDEFLIEPIRALGLRA
                     RFYGARYPQQALRSLRSAGIEYGGWIPDFCVPAALSAARFTVHVPLRLRADGLPAHVP
                     AMPVLQALACGVPVVTAPWDDCAELFESEDDLLIGRNGPEMTRHLAALRSDPEFRQFV
                     AANGYRTVMGRHTCAHRARELLSICRELRVSDSRRRSQHDDPAAVLPVFPYRPPRGQL
                     SHAPAAWN"
     gene            complement(2782..3618)
                     /locus_tag="VFB37_13660"
     CDS             complement(2782..3618)
                     /locus_tag="VFB37_13660"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23545.1"
                     /translation="MLQHTPYEAAQFILRLEAPRCAAHAQPGSFVHLTCDPSLPMRRP
                     LSIMRADPRAGWIELLYKVVGQGLKALAGRKRGEVLSTLGPIGQPFAAHPQRPRPLLV
                     GGGVGIPPMIFLAEQLRSRPDADWKPLVLMGSEIPFPFRARPSRILVAGMPEGSIACM
                     PLLEEWGIASRLASGSGFPGCFEGFVTELADCWLGSLDAAVLGEIELFACGPTAMLRA
                     AAALAERYRVPCQVSLEEFMACGVGGCAGCTVCVQTESGPAMKRVCVDGPVFEASSVF
                     GA"
     gene            complement(3666..4637)
                     /locus_tag="VFB37_13665"
     CDS             complement(3666..4637)
                     /locus_tag="VFB37_13665"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZO23546.1"
                     /translation="MSDLQIDQRRPDGSLRHLLTLENLPRREIERLLNKAESFVRPLG
                     ARPPTSNALTGVTVANLFMEPSTRTRVSFELAAKRLGADVVNLEVQLSSRVKGESMLD
                     TLYTLQSLHVDVMVIRDAERGVPALVAANVAPHVSVLSAGEAHVSHPTQGLLDALTVR
                     QYKKRFEGLSIAIVGDIRHSRVARSAHHAFSALGVTDLRIVAPPALMPEADEFSGCTR
                     HTTLDTGLVDADVVMMLRIQKERMGQADLPDADRYYNQYGLSPERLTLARADAIVMHP
                     QPMNRGIEIASEVADGPQSVIRDQVRNGVAVRMAVLADVIGSRVHTT"
BASE COUNT          908 a         1574 c         1559 g          728 t
ORIGIN      
        1 cgcggcgatg gagatcatgc tcaactcgcc gctgatccag gacctgatct tcaagggtga
       61 ggttgccaag atcaaagagg tcatgtcacg ctcgacgcgt ctcggcatga agaccttcga
      121 ccagtcgctg ttcgagctgt acgagacggg cttcatctcg tacgaggacg ctctgcgcaa
      181 cgccgactcg aagaacgagc tgcgcctgcg catcaagctc gagagcaagc gcgaaacgaa
      241 gaccatcgac gacggcgcgc tgcagatcgt cgaggaagag caggggcgat cgttgtaagg
      301 actcccgccg accatggcag ccaacacgac gccggcgaaa gagccggcgg attcgaagac
      361 gacccccgcc gcacgcgagg cttcactcgc gaccacggga acgtatgcgg ttgcgagcaa
      421 cggcagccat ccgactctca acaccaagcc gctcttcaag ctgatggtgg agaagaaggc
      481 ctccgacctg ttcttcacct ccaacgcgcc catcaagatc aagatcgagg gacagatcct
      541 gccggtcaac aagcaggtgc tgacccccga gaccgtgcgg cagaccgcct tcgcgctgat
      601 gacggccgaa cagcgcgaag agttcatccg gcagttcgag ctggacttcg ccatctccga
      661 gcccgggctc gggcgcttcc gcgtcaacgt attcatgcag cgcggctttc ccgcgatggt
      721 gctgcgctat atcaccgccg acatgccgcg cctggagagc ctcgggctgc ccgagatcat
      781 gacggacctg gtgctgctca agcgcgggct gatcctgatg gtgggcgcga ccgggtcggg
      841 caaatcgacc tcgctcgcgg cgatgatcaa ttaccgcaac gagaacgcct cggaccacat
      901 cgtcaccatc gaggatccca tcgagttcct gcacaccaac aagcgctcgc tcgtcaacca
      961 gcgcgaggtg ggaatcgata ccaagagcta ctcgcgcgcg ctgcgcggag tggtgcgcgc
     1021 tgcgcccgat gtcatcctga tcggtgagat ccgcgatcgc gagagcatgg aagccgcgat
     1081 tcatctggcc ggcaccggac acctggtgct cgcaacgctg catgccaaca actgcgcgga
     1141 atccctcgac cgcatcatca acatgttccc gcgcgagcag cacaaccaga ttttcctgga
     1201 catgtcgcag tacctgcgcg ccattctggc gcagcgcctg gtgatgggcc gcgacggcaa
     1261 gcggctggcc gccgtcgaag tgatgatcaa cactccgcac atctccgagc tggtcaagaa
     1321 gggggatgtc atcgggatca aggaggcgat cacgacgagc ggcgagcggg gcattcaaag
     1381 cttcgacgtc tcgctgcgcg acctgcacaa gcagggtcgt atcgatctgg aagaggcgct
     1441 cgcgagcgcc gactcgcgcg ccaatctcga ggccaagatc aacttcggct gagtctcagt
     1501 tccatgcggc cggcgcatgg gagagctgcc cgcgtggcgg ccggtaggga aagacgggca
     1561 gcacggcagc cgggtcatcg tgctggctgc gccggcgcga gtcgctcacc cgcagctcgc
     1621 gacagatgga cagcagctcg cgcgcccggt gagcgcaggt atgccggccc atcacggtgc
     1681 ggtagccgtt tgccgccacg aactgccgga actcgggatc gctgcgaagc gcagcgaggt
     1741 ggcgagtcat ctcaggcccg ttccgtccga tcagcaggtc gtcctccgat tcgaacagct
     1801 ccgcgcagtc atcccaggga gcggtcacga ccggtacgcc gcaggcgagc gcctgaagaa
     1861 cgggcattgc cgggacgtga gcaggcaggc cgtctgctcg caggcgcagc ggcacgtgca
     1921 cggtgaagcg tgccgcactc agtgccgcgg ggacgcagaa gtccgggatc cacccgccgt
     1981 actcgatgcc ggcggagcgc agtgagcgca aagcctgttg cggatagcgc gcaccgtaga
     2041 agcgcgcacg caggccgagg gcacggatgg gctcgatcag gaactcatcg agctcaccca
     2101 gacgctcacc tacgtcccag cttcccaccc agatcagatc acgtgagagc gcgggctcgc
     2161 ctggggacgg cctgaataca cgaacatcga ccgcgtcgcg ccacgtccac cggttgggac
     2221 catcctgctc tcggtaggtc ctgcgcagcg catcgctcgc ggccagtacg ccatcgaaag
     2281 cttgcaacgc ctgcgtgcgc acctcgaggg acatccgtgc accactatgc ggcgagtcgt
     2341 gaaagagcag ccggtacgcg cgcgatcggg cgcgatgctc cccgaggcgg cgaaggaatc
     2401 cgggctcgtt ccattcatgg gcgatgacga catccgcatc ggccagcgcg ccgtccagat
     2461 ccagtgtcag ggggtcatag aagctgctgc gaagggtggg atacgtcgag tggaatgcct
     2521 tcaccactcc atcgccgccc tgctcgcgca ggttatgcag gctccagccg tcggagggct
     2581 cgaagatgcg cacggcatgt ccgctcgcga gcagctcgga ggcgaccccg cgaagcatgt
     2641 gggcgctggg atgattccag tcggagacga gcgagtgata gaagagaacg aagcgcatgg
     2701 ctgctcttcc atggcaaggc ccatccaccc gcgcaagcaa ttagcgttcc cgccgtgtgc
     2761 ctctgagctc tcaggggccg atcacgcgcc aaacaccgaa gaagcttcga acaccgggcc
     2821 gtccacgcag acacgcttca tcgccgggcc ggattcggtc tgcacgcaaa ccgtgcagcc
     2881 tgcacagccg ccgaccccac aggccatgaa ctcctcgagc gagacctgac agggcacgcg
     2941 gtaacgctcc gccagcgcag cggctgcccg gagcatggca gtcggaccgc aggcgaagag
     3001 ctcgatctcg cccagcaccg cggcgtcgag gctcccgagc cagcagtccg caagctcggt
     3061 cacgaagccc tcgaagcagc ccgggaaccc ggagccgctg gccaaccggc tggcgatacc
     3121 ccattcctcg agcaggggca tgcacgcaat gcttccctcg ggcatcccag ccacgaggat
     3181 gcgcgagggt cgcgcgcgga acgggaaggg gatctcggag cccatcagca ccagcggctt
     3241 ccagtccgca tccgggcgcg agcgcagctg ctcggccagg aagatcatcg gcgggatgcc
     3301 gactccaccg cccacgagca gtgggcgcgg ccgctgcgga tgcgcggcaa aaggctggcc
     3361 gatcgggccg agcgtgctga gcacctcacc ccgcttccgg cccgccaggg ccttgagccc
     3421 ctggccgact accttgtaga gcagctcgat ccatccggct cgcggatcgg cgcgcatgat
     3481 ggacagcggc cggcgcatcg ggagcgaagg atcgcaggtg aggtgcacga agctccccgg
     3541 ctgcgcgtgg gcggcgcagc gcggcgcttc gagccgaagg atgaactggg cagcttcgta
     3601 gggcgtgtgc tgaagcacgc gcgcctcctc gaggaagagc gtaccgcggt gagggcgagc
     3661 cgcgctcaag tggtgtggac ccgggagccg atgacatccg ccaacaccgc catgcgcacc
     3721 gcgacgccat tacgcacctg atcacggatg acggattgcg gtccatcggc tacttcggag
     3781 gcgatttcga tgccgcggtt catgggctgc ggatgcatga caatggcgtc ggcgcgggcc
     3841 agtgtcagtc gctccgggct gagcccgtac tgattgtagt aacgatccgc gtcggggaga
     3901 tccgcctgtc ccattcgctc cttctggatg cgcagcatca tgaccacgtc cgcatccacc
     3961 agcccggtgt cgagcgtcgt gtgtcgcgtg cagccggaaa actcatccgc ttccggcatg
     4021 agcgcaggcg gtgcgacgat gcgcagatcg gtgaccccga gggcactgaa tgcatgatgg
     4081 gccgagcgcg cgacacgtga gtgccggatg tcgccgacga tggcgatgct caggccctcg
     4141 aaccgttttt tgtactgccg cacagtaagc gcatccagga gcccctgcgt gggatgcgaa
     4201 acatgcgcct cacctgcgga gagcacgctc acgtggggcg caacgttcgc agctacgagt
     4261 gccggcactc cgcgctcggc gtcacgaatc accatgacat cgacgtgcag ggactgaagg
     4321 gtatagaggg tatcgagcat gctctcccct ttcacgcgcg atgagagctg cacctcgaga
     4381 ttcaccacgt ccgccccgag tcgcttggct gccagctcga aagacacacg agtgcgtgtg
     4441 gagggctcca tgaagagatt cgcgacggtg accccggtga gggcgttgct ggtgggcggg
     4501 cgcgctccca ggggtcgcac aaagctctcg gctttgttga gcagccgctc gatctcgcgt
     4561 cggggcaaat tctcgagcgt cagcaggtgg cgcaggcttc catccggcct gcgctggtcg
     4621 atctgcaaat cactcatgtg gacgcccctc gccggccagc cagcgctcca ggatcacggc
     4681 cgccgcggca ctgtcgatgt cttcgcgctg gactcgccgg cggcgcacgc cgctcgcgcg
     4741 ccgcatcttc agagctgtgg ccgcttcca
//
LOCUS       DASRHN010000341         8611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2099612, whole genome shotgun sequence.
ACCESSION   DASRHN010000341 DASRHN010000000
VERSION     DASRHN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099612"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFB37_13670"
     CDS             <1..397
                     /locus_tag="VFB37_13670"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZO23547.1"
                     /translation="GFAQSAIFNMPNKRHRDKRGGARFPATDRSKSLRRTHSVKDLLG
                     RLTPTLTRVSDQARTQTFWQMWLAEQLPAALCQRVSAVEESDGTLVIYAESAAWSARL
                     RFAMQDLEPDARAARRSIRQISVRVLPRT"
     gene            complement(402..1322)
                     /gene="lpxC"
                     /locus_tag="VFB37_13675"
     CDS             complement(402..1322)
                     /gene="lpxC"
                     /locus_tag="VFB37_13675"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZO23548.1"
                     /translation="MVGQRTLKNSIRATGVGLHTGRKVLMTLRPAPADSGIIFRRTDM
                     ADALDIPARAENVGDTMLGTTLMRGEARVSTVEHLLSAFAGLGIDNAIVELSAAEVPI
                     MDGSAGPFVFLLQSAGIEEQRRPKRFIRIKKRLRAEDGAKWAQFDPFDGFKVNFEIEF
                     NHPIFKRRSQRASMDFSTTSFLKEVSRARTFGFMRDLETLRAHNLALGGNLDNAIVLD
                     DYRVLNEDGLRYEDEFVKHKILDAIGDLYLLGHSLIGEFSGHKSGHALNNALLRMLLA
                     DTSTWEEVSFNSYADAPISYIEAPVLPGSG"
     gene            complement(1506..2732)
                     /gene="ftsZ"
                     /locus_tag="VFB37_13680"
     CDS             complement(1506..2732)
                     /gene="ftsZ"
                     /locus_tag="VFB37_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZO23549.1"
                     /translation="MFELMDAYSQSAVIKVIGVGGGGGNAVAHMVTSGIDGVEFMCIN
                     TDAQALKHAKVKTALQIGCNITKGLGAGADPEVGRQAAMEDRDRVVELVEGCDMLFIT
                     AGMGGGTGTGAAPVVAQVAKELGILTVAVVTKPFEMEGNKRSYVADHGIAELGKYCDS
                     LITIPNQKLLTVLGPQTTLLDAFKSANQVLQGAVQGIAELITRPGLINVDFADVRTVM
                     AETGMAMMGSGAASGEGRAREAAEAAVSSPLLEDINLAGAHGILVNVTAGMDLSIGEF
                     QEVGQIVKQFASEDATVVVGTVIDPELSNQVRVTVVATGLGRPAAAVRPTPAQHQHRD
                     MAPPRESAMHRDPMHSRSEPPMRVIRRGPLSSSDYAALDKPTVQRQRAVGDGLRPEGE
                     PDDLLDIPAFLRRQAD"
     gene            complement(2817..4052)
                     /gene="ftsA"
                     /locus_tag="VFB37_13685"
     CDS             complement(2817..4052)
                     /gene="ftsA"
                     /locus_tag="VFB37_13685"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZO23550.1"
                     /translation="MLKRSERNLIVGLDIGTSKVVSLVGEVGLDGSIEVLGLGSHASR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCEIHSVFAGIAGSHVRSLNSHGVVAIRDK
                     EVTHGDVDHVIDAAKAVAIPADQKILHVLPQEFIVDGQEGIRDPIGMSGVRLEAKVHI
                     VTGADSAAQNIEKCIQRCGLEVDDVVLEQLASSFAVLTEDEKELGVCLVDIGGGTTDL
                     AVFANGAIRHTAVIPIAGDQVTNDIAVSMRTPTQYAEDIKIRYACALSQLANPDESIE
                     VPSVGDRPARRLARQTLAEIVEPRYEELFGLVREELRRSGFEEVIAAGIVLTGGSAKM
                     EGAIELAEEVFHVPVRLGLPQSIRGLSDVVRNPIYSTGVGLLLYARDNVMPARRGRTL
                     GGNAKTVLDRMRSWFQGNF"
     gene            complement(4045..4821)
                     /locus_tag="VFB37_13690"
     CDS             complement(4045..4821)
                     /locus_tag="VFB37_13690"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZO23551.1"
                     /translation="MRRNRLKQTERRWKLPTINWRAWAMSVGSLTAICGTVALVLWTL
                     DQPIESVSVSGQFQRVAPSDVERIVKQQVRGAGLLSVNLEAVRHSIHTLPWVDAVSVQ
                     RAWPRGLHVLVIEQVAVARWGDTGLLNTRGELFSKDERHMPPELPELSGPDGSEASVA
                     QRYLAAQGRLIPLGLRLTALRLDARGAWEFDLSDGVTVRLGRRQVDERFDKFINAAAK
                     LVTQRGDDISYVDMRYTNGFAIGWRHGATHSAKENGGRDA"
     gene            complement(4818..5810)
                     /locus_tag="VFB37_13695"
     CDS             complement(4818..5810)
                     /locus_tag="VFB37_13695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZO23552.1"
                     /translation="MRLTRAPAFAHRASAASEFGHVAVLLGGDSSEREISLLSGNAVL
                     EGLLRRGIDAHAFDPGEQALSTLLDERFDRVWIALHGPGGEDGTVQGALEYLGVPYTG
                     SGVMGSAIGMDKLRTKRLAQAIGVATADYVSLRGPQDFEIAIDRLGLPLIVKPATQGS
                     SVGMSKVERAEELPAAYEAAARVESSVFAEPWISGKEYTVAILQGRALPAIRIETPKT
                     FYDYEAKYFRDDTRYYCPCGLSGAAEQHLASLALAAFEAVDAEGWGRADFMTDAAGRP
                     LLLEVNTIPGMTSHSLVPMAARADGIDFDELVWRVLETSFVRRPQDDVPGGPGA"
     gene            complement(5810..6700)
                     /gene="murB"
                     /locus_tag="VFB37_13700"
     CDS             complement(5810..6700)
                     /gene="murB"
                     /locus_tag="VFB37_13700"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZO23553.1"
                     /translation="MAVAPEFVLRVRRNEPMSRHTSWHVGGPAEVFFNPRDRADLAAF
                     LRSLGEDVPIHWIGLGSNLLVRDGGLRGVVISTLGTLDRLDRLNQITVYCEAGVACAR
                     LAKQCVKWGLGPAEFFAGIPGTLGGALAMNAGAFGGETWHHVVEVQTMDRRGREHIRT
                     AAEYEVGYRHVVGPAPEEWFIGAKLKFEHKPGINETRVRELLERRKASQPIGEWSCGS
                     VFTNPPGDHAARLVEAAGLKGYRLGDASVSEKHANFIINHGHASAADLERLIGYVQET
                     VERQHGVRLNTEVRIMGAPN"
     gene            complement(6703..8115)
                     /gene="murC"
                     /locus_tag="VFB37_13705"
     CDS             complement(6703..8115)
                     /gene="murC"
                     /locus_tag="VFB37_13705"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZO23554.1"
                     /translation="MRRINTIHFVGIGGSGMSGIAEVLLNLGYTVQGSDTKANEVTRQ
                     LERLGARISIGHDAANVAGADVIVVSSAISRGNPEVEAALEHRIPVVARAEMLGELMR
                     FRYSIAVAGTHGKTTTTSLVASILAEGGEDPTFVIGGRLQSAGSNARLGAGRYLVAEA
                     DESDASFTHLQPLIAIVTNIDNDHLVTHGGDFELLKQSFIEFLHNLPFYGLAVLCLDD
                     PNVRSVVRAVGRPIVSYGIASEDADVRAVNIRREGPRTSYDVQRDEAEPLRVTVNLPG
                     EHNVLNSLAAIAVASELGIDDQAIQRALSSFQGVERRLQHVADVRTSSGRITIVDDYG
                     HHPTEVLATLEALRQAYPGRRLVLAFQPHRYTRVHDLIDDFSRALSSADALLVTEVYA
                     AGETPIAGADGRAICRAVRGRGLVEPVFVEKVEDLASSLAEVIRDGDVIVTMGAGHIS
                     VVSHDLPARLGISSHDAEGS"
     gene            complement(8205..>8611)
                     /locus_tag="VFB37_13710"
     CDS             complement(8205..>8611)
                     /locus_tag="VFB37_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO23555.1"
                     /translation="GRQSYASAGVRADVRPFIEDMAEAYSWADLVICRSGALTVSELA
                     AAGVGAILVPFPAAVDDHQTQNARYLVGEGAAVLIADRELSAERLAEELQRLCAGRAK
                     LLAMAERARQLAKPHAADDLAASCLEIAGSAA"
BASE COUNT         1460 a         3021 c         2668 g         1462 t
ORIGIN      
        1 cgggtttgcc caatcagcga ttttcaatat gcccaataag cgacaccgag acaagcgcgg
       61 cggtgcccgc tttccggcga cggaccgctc aaaatcgctg cgccgcaccc attctgtcaa
      121 agacctgctg gggcgcctga ctcctacatt gacacgggtc tcggatcagg cccgcaccca
      181 gacattctgg caaatgtggc tcgcggagca gctgccggca gcattatgcc agcgcgtctc
      241 cgcagtcgag gaatccgacg gaacgctggt gatctacgcg gagtcggctg catggtcggc
      301 acggctgcgt tttgcgatgc aggatctcga gcccgacgcg cgtgccgcgc gccgcagcat
      361 ccgccagatc agcgtgcgcg tgctaccgag gacgtgagga atcagcccga gccgggcaga
      421 acaggcgcct cgatgtagga aatcggcgca tcggcgtagg agttgaagga cacctcttcc
      481 cacgttgagg tgtcggccag cagcatgcgc agcagcgcgt tgttgagcgc atggccggac
      541 ttgtggccgc tgaactcgcc gatcaggctg tgcccgagca ggtacaggtc gccgatcgca
      601 tcgaggatct tgtgcttgac gaactcatcc tcgtaccgca ggccatcctc gttcagtacg
      661 cgatagtcat cgagcacgat ggcgttatcg aggttgcccc cgagcgccag attgtgcgcg
      721 cgcagggtct ccaggtcgcg catgaaaccg aaggtgcggg cgcggctcac ttccttgagg
      781 aaggacgtcg tcgagaaatc catcgaggcc cgctgcgagc ggcgcttgaa gatcggatgg
      841 ttgaactcga tctcgaagtt caccttgaac ccatcgaacg gatcgaactg cgcccacttc
      901 gcgccgtcct ccgcgcgcag gcgcttcttg atccggatga aacgcttggg ccgccgctgc
      961 tcctcgatgc cggcggactg gagcaggaac acgaacggtc ccgcactgcc atccatgatg
     1021 ggcacttccg ccgcgctcag ctcgacgatc gcattgtcga tgccgagacc ggcgaacgcg
     1081 gacaggaggt gctcgacggt cgagactcgc gcctcgcccc gcatcagcgt ggtacccaac
     1141 atcgtgtcgc cgacattctc ggcgcgcgcc ggaatgtcca gcgcgtccgc catatcggtc
     1201 ctgcggaaga tgattccgga atcggcggga gcgggacgaa gcgtcatcag aaccttgcgg
     1261 ccggtgtgca gacccacacc cgtggcacga atcgagttct tcagcgtccg ttgtccgacc
     1321 atgcttccgc ttacttgcat caaaggagcc gccgtggggg tatgtgcccg aagacgagct
     1381 ggcatacccg ccacggcgca agaaggcgcg ttaccgtatc acaggcgaaa aaacgactcc
     1441 aatcaagact gcgaagagca tcctgcagcg cttcccgacc cgggcatcct gcccgggctc
     1501 gcggctcagt ccgcctggcg ccgcagaaac gccggaatgt ccagcagatc gtccggctca
     1561 ccttcgggcc gcagaccatc gccgaccgcg cgctgccttt ggaccgtggg cttgtccagg
     1621 gcggcgtagt cgctgctcga caggggtccg cgccggatga cccgcatcgg cggctctgat
     1681 cgcgagtgca tcggatcgcg atgcatcgcg ctctcgcgag gcggagccat atcccggtgc
     1741 tgatgctgtg cgggtgtggg ccgcaccgcc gcagccggcc ggccgaggcc ggtcgcgacc
     1801 accgtcacgc gcacctggtt ggaaagctcc ggatcgatga ccgtgccgac caccaccgtc
     1861 gcatcctccg aggcgaactg cttgacgatc tgtcccacct cctggaactc gccgatcgag
     1921 aggtccatcc ccgccgtcac gttcaccagg atcccgtgtg caccggcgag gttgatatcc
     1981 tcgagcaacg gtgacgagac tgctgcctcg gccgcctccc gcgcgcgtcc ctcgccgctg
     2041 gccgcacccg agcccatcat ggccatgccg gtctcggcca tcaccgtccg cacgtccgca
     2101 aagtccacat taatgagtcc cggacgcgtg atcagttccg cgatcccctg caccgcgccc
     2161 tgcagcacct ggttcgccga cttgaacgca tccagcagcg tcgtctgcgg tccgagcacc
     2221 gtgagcagct tctggttcgg aatcgtgatc agcgaatcgc agtacttgcc cagctccgcg
     2281 atgccgtgat cggcaacgta cgagcgcttg ttgccctcca tctcgaaggg cttggtgacg
     2341 acggcgaccg tcaggatccc gagctccttc gccacctgcg ccaccaccgg cgcagcaccc
     2401 gtgccggtcc caccgcccat cccggcggtg atgaacagca tgtcgcagcc ctcgacgagc
     2461 tcgactaccc gatcacggtc ctccatggcc gcctggcgcc ccacttcggg gtcggccccg
     2521 gctcccaggc ccttggtgat gttgcacccg atctgcagtg cggtcttcac cttggcgtgc
     2581 ttgagagcct gtgcatcggt gttgatgcac atgaactcca ccccgtcgat cccgctcgtg
     2641 accatatgag ccacggcatt cccgccgccg ccccccacgc cgatcacctt gatgacggcg
     2701 ctctggctat atgcgtccat cagttcaaac atgcttcgcg ctcctcttac acagtcttgg
     2761 ttggtcgaac cccgtgggtt ctgtcgtttc ttcgtcactt cgcctcgtct gtcagctcaa
     2821 aagttgccct ggaaccagga acgcatgcgg tccaacaccg tcttcgcatt cccgcccagg
     2881 gttcgcccgc gacgtgcggg catgacgttg tcacgtgcgt agagcagtag ccccacgccc
     2941 gtggaataga tcggattgcg caccacgtcc gacagtcccc ggatggactg cggcaggccg
     3001 agccgcaccg gcacgtggaa tacttcttcg gccagctcga tcgcgccctc catcttggcg
     3061 ctgccgccgg tgagcacgat gcccgccgcg atgacttcct cgaagccgct gcggcgcagc
     3121 tcctcgcgca ccagaccgaa cagctcctcg tagcgcggct cgacgatctc cgccagcgtc
     3181 tgccgcgcga gccgccttgc gggccggtcg ccgacgctgg gcacctcgat gctctcgtcg
     3241 ggattggcaa gctgcgagag ggcgcacgca tagcggatct tgatgtcctc cgcatactga
     3301 gtcggcgtgc gcatcgagac cgcgatgtcg ttggtcacct ggtcaccggc gatcggaatg
     3361 acggccgtat ggcggatggc gccgttggcg aacacggcca gatcggtggt gccgccgccg
     3421 atgtccacca gacacacgcc gagctccttc tcatcctcgg tcagcaccgc gaagctcgaa
     3481 gcgagctgct cgagcaccac gtcatccact tccagcccgc agcgctgaat gcacttctcg
     3541 atgttctgcg cggcgctgtc ggcgccggtg acgatgtgca ccttcgcctc gagccgcacc
     3601 cccgacatgc cgatgggatc gcgaatcccc tcctggccat cgacgatgaa ttcctgcggc
     3661 agcacgtgca ggatcttctg atcggccgga atcgctaccg ccttggcggc atcgatgacg
     3721 tgatcgacat ccccgtgcgt cacttcttta tcccggatcg cgaccacgcc gtgggagttg
     3781 aggctgcgca cgtggctgcc ggcgataccg gcgaacaccg agtgaatttc gcagccggcc
     3841 atcagctcgg cctcttccac cgcgcgctgg atggactgca ccgtggactc gatattgacg
     3901 accacgcctt ttttcaggcc gcgtgaggcg tgcgagccga ggcccagcac ctcgatggag
     3961 ccatcgaggc ccacctcgcc caccagcgac acgaccttgg aggtgccgat gtccaggccc
     4021 actatcaggt tccgctcgga gcgcttaagc atcccgacca ccgttttctt tagcactgtg
     4081 cgttgccccg tggcgccagc cgatcgcaaa tccattcgtg tagcgcatgt cgacatagga
     4141 aatatcgtct ccgcgttgtg tcacgagctt cgccgccgcg ttgatgaact tgtcgaagcg
     4201 ctcatccacc tggcgcctgc cgaggcggac ggtcactcca tccgacagat cgaattccca
     4261 cgcgccgcgc gcatcgaggc gcagcgcggt cagacgcaag ccgagcggga tcagccggcc
     4321 ctgggccgcc agatagcgtt gcgccactga ggcttcgctg ccgtccgggc cggaaagctc
     4381 cggcagctcc ggcggcatgt gccgctcgtc cttcgagaac agctccccac gcgtgttgag
     4441 gagtccggta tcgccccagc gcgccaccgc cacctgctcg atcacgagca cgtgcaggcc
     4501 gcggggccac gctctttgca cgctcaccgc atcgacccac ggaagggtgt gaatcgaatg
     4561 gcgcacggcc tcgagattca cactcagcaa gccggcaccg cgcacctgct gcttcacgat
     4621 gcgctcgaca tccgaaggcg cgacgcgctg gaactgaccc gataccgaca ccgattcaat
     4681 cggctgatcg agtgtccaca gcaccagtgc cacggtgccg cagattgcgg tcagactccc
     4741 caccgacatc gcccacgcgc gccagttgat cgtgggaagc ttccagcgcc gctcggtctg
     4801 cttgagccga ttgcgtctca tgcgcccggg cctcccggga cgtcatcctg gggacggcgc
     4861 acgaagctcg tctcgaggac ccgccagacg agctcgtcga agtcgatgcc atccgctcgg
     4921 gcagccatcg gcacgaggct gtggctggtc atgccgggaa tcgtgttgac ttccagcagc
     4981 agcggccggc cggctgcgtc cgtcatgaaa tccgcgcggc cccagccctc cgcatccacg
     5041 gcttcgaacg ccgccagcgc aaggctcgcc aaatgctgct cagcggcccc cgacagcccg
     5101 cacgggcagt agtagcgcgt atcgtcgcgg aagtatttcg cctcgtagtc gtaaaaggtc
     5161 ttcggcgtct cgatgcgaat ggcggggagc gcgcgcccct gcaatatcgc aaccgtgtat
     5221 tccttgccgg agatccacgg ctcggcaaac acactgctct cgacccgcgc cgccgcttcg
     5281 tacgccgcag gcagctcttc agcacgctcg actttggaca tgccgacgct cgagccttgc
     5341 gtcgccggtt tcacgatcag cggcaatccc agccgatcga tggcgatctc aaagtcctgc
     5401 ggcccacgca gcgacacgta gtcggcggtg gcgaccccaa tggcttgggc gaggcgcttg
     5461 gtacgcagct tgtccatccc gatggccgag cccatgacgc cactgccggt atacggaacg
     5521 cccaggtact cgagtgcgcc ctgcacggtg ccgtcctcgc cgccaggccc gtgcagcgca
     5581 atccagactc ggtcgaaacg ctcatcgagc agtgtggaga gggcctgctc gcctggatcg
     5641 aacgcgtgtg catcgatgcc gcgccgaagc agaccctcga gaaccgcgtt gccgctcaga
     5701 agcgagatct cgcgctcgct cgaatccccc ccgagcagca cggccacgtg gccgaactcg
     5761 ctcgccgcgg aggctcgatg cgcgaatgcg ggggcgcgcg tgaggcgcat cagtttggcg
     5821 cccccatgat gcggacctcg gtgttgagtc gcacgccatg ctggcgctcg accgtctcct
     5881 gcacgtagcc gatcaggcgc tccagatcgg cagcgctcgc atggccgtga ttgatgatga
     5941 agttggcgtg cttctcggaa accgaggcat cgcccaggcg gtaacccttc aaacccgccg
     6001 cctcgaccag ccgcgccgcg tggtccccgg gcggattggt gaacaccgag ccacagctcc
     6061 actcgccgat cggctgcgag gccttgcggc gctcaagcag ctcccgcacc cgcgtctcgt
     6121 tgatcccggg cttgtgctcg aatttcaact tcgcgccgat gaaccactct tcgggagccg
     6181 gcccgaccac gtgccggtag ccgacttcgt actcggcggc cgtgcggata tgttcgcggc
     6241 cgcgccgatc catcgtctgc acttccacga cgtgatgcca ggtctcgccg ccgaaggcgc
     6301 cggcgttcat ggccagcgcc ccgccgagag ttccggggat gccggcgaag aactccgccg
     6361 ggcccagacc ccacttgacg cactgcttgg ccaggcgggc gcaggccaca cccgcctcgc
     6421 agtacacggt gatctgattg aggcggtcga gacgatcgag cgtgccgagg gtgctgatga
     6481 ccacgccccg caagcccccg tcgcgaacca acagattgct gccgagtccg atccagtgaa
     6541 tcggcacgtc ctcgcccagc gagcgcagga aggcggccag gtcggcgcgg tcgcgcgggt
     6601 tgaagaagac ctccgcggga cccccgacgt gccacgaggt gtgccggctc atcggctcgt
     6661 tgcgcctgac gcgcagtaca aattcggggg cgacagccac tgtcacgatc cctccgcgtc
     6721 gtgggacgaa atcccgagcc gcgcaggcag gtcgtgcgag accacactga tgtggccggc
     6781 acccatggtc acgatcacat cgccatcgcg gatcacttcc gcgagcgagc tggccagatc
     6841 ctccaccttc tccacgaaca ccggctcgac cagtccacgc ccgcgaacgg cgcggcagat
     6901 ggcgcggcca tcggccccgg caatgggtgt ctcacccgcc gcatagactt cggtcaccag
     6961 aagcgcatcg gcgctggaaa gcgccctgct gaagtcatcg atgagatcgt gcacgcgcgt
     7021 gtagcgatgc ggctggaagg cgagcacgag gcgccggccc ggatacgcct ggcgcaacgc
     7081 ctcgagcgtg gcgagcactt cggtcggatg gtggccatag tcgtccacga tcgtgatgcg
     7141 accgctgctc gtgcggacat cagccacgtg ctgcagccgg cgctcgaccc cctggaagct
     7201 cgagagcgcg cgctggatcg cctgatcgtc gatccccagc tcactcgcca ccgcaatcgc
     7261 ggccagcgag ttcagaacgt tgtgctcgcc tggcaggttg acggtgactc ggagcggctc
     7321 ggcctcatca cgctggacat cgtaggaggt tcggggcccc tctcggcgaa tgttcacggc
     7381 gcgcacatcc gcatcctccg aagcgatccc gtacgacacg atgggccgtc ccactgcgcg
     7441 caccacgctg cgcacgttcg gatcgtcgag acacagcacg gcgagcccgt agaaaggcag
     7501 gttgtgcagg aattcgatga agctctgctt gagcagctcg aagtcgccac cgtgggtgac
     7561 cagatggtcg ttgtcgatgt tggtcacgat ggcaatgagc ggctgcaggt gcgtgaagga
     7621 ggcatcgctc tcgtccgctt cagcgacgag gtagcgtccg gcaccgaggc gcgcgttgct
     7681 gccggcgctc tgtaaccgcc cgccgatcac gaaggtcgga tcttcgccgc cttcggcgag
     7741 gatgctcgcg accagactcg tcgtggtggt cttgccgtgg gtgccggcga ccgcgatcga
     7801 gtaccggaag cgcatgagct cgcccagcat ctctgcgcgg gcgaccacgg gaatgcgatg
     7861 ctcgagcgca gcctccacct ccggattgcc gcgcgagatc gcgctcgaga ccacgatgac
     7921 atcggcgcct gcgacattcg ccgcatcgtg gccgatcgag atgcgcgctc cgagcctctc
     7981 gagctgccgg gtgacctcgt tcgccttggt gtcggaaccc tgaaccgtgt agccgagatt
     8041 gagcagcacc tcggcgatcc cactcatccc gctgccgccg atcccgacga agtggatggt
     8101 gttgatccgc cgcatccgat cgcgccgatc gggagccacc cgacgctcca ctgcgggtct
     8161 ttccagcacc cggcgacgct cacccccgct gcggcgttcg atcatcatgc cgcgctcccg
     8221 gcgatttcca ggcaggacgc ggcgagatcg tcggcggcgt gcggcttggc cagctgtcgc
     8281 gcgcgctcgg ccatggcgag cagcttggcg cgcccagcgc acaggcgctg cagctcctcg
     8341 gccagccgct cggcactgag ctcacggtct gcgatgagca ccgcggcccc ctcgccgacc
     8401 aggtagcgcg cgttctgagt ctggtgatcg tccacggccg ccgggaaggg aaccagaatc
     8461 gcaccaacac ccgctgccgc cagctccgac acggtgagcg ccccggaccg gcagatcacc
     8521 agatcggccc aggagtaggc ctccgccatg tcctcgatga agggccgcac atcggcgcgc
     8581 acgccagcgc ttgcgtagct ttgccgcccg g
//
LOCUS       DASRHN010000342        13774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_285666, whole genome shotgun sequence.
ACCESSION   DASRHN010000342 DASRHN010000000
VERSION     DASRHN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285666"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFB37_13715"
     CDS             <1..906
                     /locus_tag="VFB37_13715"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZO23556.1"
                     /translation="AYIYRALVLVTRPVLWLTNRMAYGLLWIVGVGQTSRASQALSTE
                     ELRTVVVEAGPMIPARHRQMLLSILDLGQVTVNDIMIPRQEIAGIDVGENWDDILDQL
                     RQTPHTRLPVYEAELDKLYGILHMKRVAQELARGTLTRERLIEVARNREPYFVPESTP
                     LTVQLSQFQRNRRRLAFVVNEYGDIEGLVTLEDILEEIVGEFTTDPATVVHKDIYIER
                     PGVFIVNASATIRAVNRSLQWHLPTDGPKTINGLLLEQLEMIPNSGTALKVGDYQFEV
                     LQLADNAIKTVRIFAPTTDEVTATG"
     gene            complement(890..2239)
                     /gene="radA"
                     /locus_tag="VFB37_13720"
     CDS             complement(890..2239)
                     /gene="radA"
                     /locus_tag="VFB37_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZO23557.1"
                     /translation="MGRASAVFTCSACGGETLKWQGQCPLCGEWNSLEQAAPRPGRPK
                     AQGATPQRLETATSGRQARLASGQDELDRVMGGGIVPGSVTLLGGDPGIGKSTLLLQV
                     AAHVAGTSPVLYASGEESVSQIALRAGRLGIEGSRVELVTESGLEAILDLTAQRKSAL
                     LVVDSIQTTQSGAVASSAGAVSQLRECTAELVRFAKSSGTAVLIIGHVTKEGAIAGPR
                     VLEHLVDTVLYFESEAGSRYRIVRATKNRFGAVNELGFFAMTESGLREVRNPSAIFLA
                     RAPEPAAGSIVTVTREGGRPLLIELQALVDRMRFGAPRRVAQGLDANRLAMLLAVMNR
                     HAELSLQEHDVFVNAVGGIQIGETAWDLPVAIALASSLKDAPVPNNLVCFGEIGLTGE
                     VRPVAYGEERLREAHKQGFQLAIVPRENAPRKPLEGLRVIAVPRLAPALEAAFSPSR"
     gene            2419..3243
                     /locus_tag="VFB37_13725"
     CDS             2419..3243
                     /locus_tag="VFB37_13725"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZO23558.1"
                     /translation="MSLRSRLRFVGLTDTGKVREHNEDTIAFDADIGLLVLADGMGGY
                     NAGEVASGIAVKTIVNLVREQVEREDMNLQDRESGLSRPAIILRDAIHRANKIIYQTA
                     RTQPQCEGMGTTVVAALFFDNRITIAHVGDSRLYRQRSDKLEQVTMDHSLLQELVDRG
                     FYSAEEAQRAANKNYVTRALGVEPNVDVEIQEVPVQKGDAYMLCSDGLSDMVEDEDIH
                     LTINTFGDNLDTVAKQLIQLANDNGGRDNVSVVMAQIIEAFPARTRIFDRILGWFG"
     gene            3257..3967
                     /locus_tag="VFB37_13730"
     CDS             3257..3967
                     /locus_tag="VFB37_13730"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZO23559.1"
                     /translation="MARLVLSLDGQVMAEYNMNKERYTIGRLPDNDIRIDNAAVSGHH
                     SLIINILNDSFLEDLNSTNGTYVNGKLIKKHALQHGDVVTVGHHQLRFVEDDEAQDEF
                     EKTMVIQPSSRPVEKVRAANEAAEQASPSLSATGRSRALSDGAALKKAKLQVLSGAFA
                     GRELELNKALTTLGRPGVQVAAITRRAEGYYIVHVDSGKENDFPLLNGTAIGAQATRL
                     ADNDVIQLAGVKMGFFVN"
     gene            complement(3960..5036)
                     /gene="alr"
                     /locus_tag="VFB37_13735"
     CDS             complement(3960..5036)
                     /gene="alr"
                     /locus_tag="VFB37_13735"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZO23560.1"
                     /translation="MSRLIRAVIDAHALRHNLRTIRERARGARVMAVVKANAYGHGLV
                     PTSLALAEADAFAVARLEEGLALRGAGITQPIVLLEGVFAREHLLEAARHGFDLVVHD
                     PLQIELLEEFGGAHRFLLWIKIDTGMNRLGFRPEEFPAALERVRRLQPAPLEIRLLTH
                     LARADERDDVMTREQLQRFQSAVGHLDFATSIANSAGLFGSVPLGCAWVRPGLALYGG
                     SPYPKGCGLDLGLQPVMSLETCVIATRKVVRGETVGYGGSWRAQRDSVIAIIAAGYGD
                     GLARNLPGGTPVLIDGSRAPIAGRVSMDMIAVDVTDLGGVYVGTRVMLWGNGLPVEEI
                     ARHAGTIPYELLCGVSQRVPLELS"
     gene            complement(5033..6439)
                     /gene="dnaB"
                     /locus_tag="VFB37_13740"
     CDS             complement(5033..6439)
                     /gene="dnaB"
                     /locus_tag="VFB37_13740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZO23561.1"
                     /translation="MAGPAKVRRDTGGLTLADAPVPPHSVEAEQAVLGGLLLDPAAWD
                     NVADVVISGDFYRSDHRMIFEAMGSLVAGGKPCDVVTVVGELERTAKLEGAGGLAYLG
                     SLARDTPTAANVRAYADIVRERSVLRQLVHAGTEIASSVFNNDGQTARELVDRAEQKV
                     FEIAESGFRGASQTMAARVLLPPLIDRIDEWNQNPEGMRGLPTGFIDFDRKTGGLRPG
                     DLVIVAGRPSMGKTTLAVNMAENAALDVKTAASVVIFSMEMPSEQLLMRMMSSLARVP
                     LGSIRAGRMSPDDWTRLTGATQQLGNARIFIDETPALTPTELRARARRLKREHGLDLV
                     VVDYLQLMQVPGTKENRATEIAEISRGLKALAKELAVPVIALSQLNRGVEQRQDRRPV
                     MSDLRESGALEQDADMILMIYREEVYDKNTTKRGIAEIDLAKHRNGETGQIMLTFRGE
                     FSRFENHMPDAYAEGVLR"
     gene            complement(6458..6907)
                     /gene="rplI"
                     /locus_tag="VFB37_13745"
     CDS             complement(6458..6907)
                     /gene="rplI"
                     /locus_tag="VFB37_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZO23562.1"
                     /translation="MEVILLQKVANLGNIGDRVKVRSGFGRNFLLPQGKATLATPANV
                     ARFEARRAELEVAAREHLSSAEERATALKDFKLLIPAKAGTEGKLFGSIGTSDIAEAI
                     SRAGFKVQRSEVRLPNGALRTLGEHAITLHLHADVDVPLAVSIVPEE"
     gene            complement(6917..7135)
                     /gene="rpsR"
                     /locus_tag="VFB37_13750"
                     /pseudo
     CDS             complement(6917..7135)
                     /gene="rpsR"
                     /locus_tag="VFB37_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(7244..7708)
                     /gene="rpsF"
                     /locus_tag="VFB37_13755"
     CDS             complement(7244..7708)
                     /gene="rpsF"
                     /locus_tag="VFB37_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZO23563.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIERYKGMIAAGGGHVHRLEDWGR
                     RQLTYPIAKVHKAHYVLVNIECDQKTLDDLTGAFRFSDAVLRHLVVNMDSAVTEPSPM
                     AKAADEEGDGSRRRERRDEDGGDDDRPRRDRSREESSASSADDEDSPAGESA"
     gene            complement(7906..8649)
                     /gene="rlmB"
                     /locus_tag="VFB37_13760"
     CDS             complement(7906..8649)
                     /gene="rlmB"
                     /locus_tag="VFB37_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZO23564.1"
                     /translation="MKTTETIYGVHPVRMILERRPEQVVVVMLTDRRDDPRVREIEEI
                     ARNQSRPVRRVDARALQQQLGDVAHQGVAAEISPLPPWSEDQLLDALGRVRDPLLVAL
                     DGVQDPHNLGACLRTADACGALAVIVPRDRAAQLTPVVRKVAVGAAETTPVVAVTNLV
                     RTLKLLKQAGLWVAGADSEGDKLPHEVDLKTGIVLVLGAEGAGLRHLTRQSCDWLVRL
                     PRLGAVESLNVSVAAGMLLYEAVRQRGSG"
     gene            complement(8656..10578)
                     /gene="rnr"
                     /locus_tag="VFB37_13765"
     CDS             complement(8656..10578)
                     /gene="rnr"
                     /locus_tag="VFB37_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZO23565.1"
                     /translation="MAGVVEGVVSANRAGYGFLRVEGMKDSVFIPPGEMRGVMHGDRL
                     RVKLARDASNRYLGTVEKILTRGVNSFLGTVELQGRTPWINAADRRLQLHCAVSPAEL
                     GGARSGDWVIARIIRHATSTAPAHAAVVKRLDPDRPVELATESAIARFDLPHEFSSAA
                     LREGQAWGEHVDPAEARAREDLRSLPLVTIDGEHARDFDDAVYAEPHPEGFRLIVAIA
                     DVSHYVRPGTALDVEARERGTSVYFPTRVLPMLPTALSDHLCSLAPHVDRLCFAADMV
                     ITRSGTLKHSRFYPAVMRSAARLTYTQAHEVLFEGRPAARSALGPLSDRLLILVDVYR
                     ALQKARNRRGALDFDAAEAEFVIDSAERIRAIELRSRNDAHRLIEECMIAANVAVAQE
                     LSRTHTPSLYRVHAQPEEQKLERLHSTLHALGIEAQIPENPTTRDLQAITRRLGTSEA
                     RPFIESLIVRAMPQAVYQQTNIGHFGLALSHYAHFTSPIRRYPDLVVHRTLKALIGAR
                     GPAAVHYDSGELATLGEATSRLEKRADEADRYVATFLKCTYLRERIGQTFHGIITTVV
                     EFGCFVQILAVAVDGLLHIDNLRDDDYVMEDNGHAWIGKRTRRRLRTGDSVRVVVTAV
                     NPIEGLVDLEWVDDVG"
     gene            10795..10879
                     /locus_tag="VFB37_13770"
     tRNA            10795..10879
                     /locus_tag="VFB37_13770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10829..10831,aa:Leu,seq:cag)
     gene            11683..13701
                     /locus_tag="VFB37_13775"
     CDS             11683..13701
                     /locus_tag="VFB37_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23566.1"
                     /translation="MAKDGRDPLGAFRELSFRWKHPAALRGSIRAYADRPVMLFTLST
                     DQAVSDAAVVRFPDFSEAPRNLHHFSYANDAFAPASFALEQTGTPWLLYDEHAHAAVL
                     SPAANFMIASMWSNDGTEIASGLNRSVASLPAGFRHMTLMVIGPGVNATWDAWGSALC
                     ALLGAHRPVNDADAGLRYLGYWTDHGAQYYYDYDPRLGYAGTLEALVRRYRDEAIPIR
                     YLQLDSWWYYKSLTDPRGKTGRPMNPALPAGEWNRYGGLLKYQAHPALFLDGLAGFQQ
                     KVGLPLITHNRWVDPASPYHERYRISGFAPVDLAWWKEIIGYLASAGVVTYEQDWLNV
                     IYEHSPELFSSPATNAAFTDGMANAARERGLTLQYSMALPRNFLQGARYENLTTIRVS
                     GDRLTRDKWDAALYTSRLASALGIWPWVDVFMSSEADNLLFATLSAGMIGIGDRIGAE
                     NRENLLRAVRLDGVIIKPDTPLVPLDAMYMPSSRRMPMIAAAHSEHGALHSSYVFCYS
                     RGSRAASTSFTPAQVGVSRQAYVYDMRTRTARLLQPSEVFALTLAPDETRYFVVVPLT
                     RAGISLFGDEHKFVPDGRKRIAALEDEAKLLTANVTFAAQEPSVRLFGYAPRRPTVTA
                     QTGSVGEVDFDPSSGRFEVTVSPGPGSQLEGPADDPVRRAVIALRLDR"
BASE COUNT         2523 a         4525 c         4325 g         2401 t
ORIGIN      
        1 gcgtacatct accgcgccct ggtgctggtg acgcgcccgg tgctctggct caccaacagg
       61 atggcctatg ggctgctgtg gatcgtcgga gtcgggcaga cgagccgggc aagccaggca
      121 ttgagcaccg aagagctgcg cacggtcgtg gtggaagcgg ggcccatgat cccggcgcgc
      181 caccgacaaa tgctgctctc gatcctcgat ctcggacagg tcacggtgaa tgacatcatg
      241 atcccgcgcc aggagatcgc cgggatcgac gtcggggaga actgggacga catcctcgat
      301 caactcaggc agacaccaca cacccgcctg ccggtctacg aagctgagct cgacaagctc
      361 tatggcatcc tgcacatgaa gcgggtggcc caggagctgg cccgcggcac cctgacacgg
      421 gagcggctga tcgaggtggc gcgcaatcgc gagccctact tcgtaccgga aagtaccccc
      481 ctcacggtgc aactctcgca attccaacgc aacaggcgcc gcctggcatt cgtcgtgaac
      541 gaatacggcg acatcgaggg actggtcacc ctcgaggaca tcctcgagga gatcgtgggc
      601 gagttcacga ccgatccagc cacagtcgtg cacaaggata tctacatcga gcgcccgggc
      661 gtgttcatcg tgaatgcgag tgccacgatc cgcgcggtga atcgctctct gcagtggcac
      721 ctgcccactg acgggccgaa gaccatcaac gggctgctgc tcgagcaact cgagatgatt
      781 cccaactcgg gcacggcgct caaggtcggc gactatcagt tcgaggtgct gcagctcgcc
      841 gataatgcga tcaagacggt gcggatcttc gcccccacca ccgatgaggt caccgcgacg
      901 ggctgaaggc ggcttccagc gccggtgcga gccgtggcac cgcaatcaca cgcaagccct
      961 ccagcggctt ccgcggcgcg ttctcccggg gcacgatcgc cagctggaac ccttgcttgt
     1021 gagcctcgcg cagccgctct tcaccatagg ctaccggcct gacctcgccc gtcagtccta
     1081 tctcgccaaa gcagacgagg ttgttgggga ccggtgcgtc cttgaggctg gaggccaggg
     1141 cgatcgccac gggcaggtcc caggcagtct cgccgatctg gatcccgccg accgcgttga
     1201 cgaatacgtc gtgctcctgc aaagagagct cggcatgacg gttcattact gcgagcagca
     1261 tcgcgaggcg gttggcatcg aggccctggg ccacccggcg gggcgcgccg aagcgcatgc
     1321 ggtcgacgag cgcctgcagc tcgatcagga gggggcggcc gccctcgcgg gtcacggtga
     1381 cgatgcttcc ggcggcaggc tccggagcac gcgccaggaa gatcgccgac gggttgcgga
     1441 cctcccgcag gccgctctcg gtcatggcaa agaaccccag ctcgttcacg gcgccgaaac
     1501 ggttcttcgt ggcccgcacg atccgatagc ggctgcccgc ctcgctctcg aagtacagca
     1561 ccgtatccac caggtgctcg agcaccctcg gcccggcaat cgcgccctcc ttcgtgacgt
     1621 ggccgatgat gaggaccgcg gtgccgctcg acttggcgaa gcgcaccagc tcggccgtgc
     1681 attcgcgcag ttgcgagacc gctcccgcac tcgatgccac ggcccccgac tgcgtagtct
     1741 ggatcgagtc caccaccagc agtgcgctct tgcgctgcgc agtcagatcg agaatggctt
     1801 ccaatccgga ctcggtaacg agctcgaccc ggctgccctc gatgccgagc cgcccggcgc
     1861 ggagcgcaat ctgggagacg gattcctcac cgctcgcgta cagcacgggc gatgttccgg
     1921 ccacgtgggc agcgacctgc aggagcagcg ttgacttgcc gattcccgga tcacctccca
     1981 acagagtcac cgatccgggt acgatgccgc cacccatgac acggtccagc tcatcctggc
     2041 cgcttgcgag ccgcgcttgc cggccgctcg ttgccgtctc cagacgctgc ggcgtcgcgc
     2101 cctgggcctt tggacgcccg gggcggggcg cggcttgctc gagcgaattc cactcgccgc
     2161 acaggggaca ctgcccctgc cacttcaggg tttcgccgcc acaggcgcta caggtgaata
     2221 cggcactcgc gcgacccatc tatccgctca ggtgtcaact ggttaggatg cgcggccaag
     2281 gccggccgct cttcgtccag tttgtgaaca gacgcctgga ctgcctgggg ggcaatgcta
     2341 gcgtatgccg gctcggatcg gggacgggaa agtgacgcgt atcacattta gcggaccctc
     2401 cggatgtcag gccgcagcat gagcttgcgt agcaggctgc gctttgtcgg actgaccgat
     2461 accggaaagg ttcgcgagca caacgaggac accattgcgt tcgatgcgga tatcggcctg
     2521 ctggtcctgg cggacgggat gggcggctat aacgcgggcg aggtggccag cggtattgcc
     2581 gtgaagacca tcgtgaatct cgtccgggag caggtggagc gggaggatat gaacctgcag
     2641 gatcgcgaat ccggcctgtc gcggcctgcg atcatcctgc gcgatgcgat tcatcgagcg
     2701 aacaagatca tctatcagac cgcgcgcacg cagccgcagt gcgagggaat gggaaccacc
     2761 gtggtcgcgg cgctgttctt cgacaacaga atcacgatcg cgcatgtagg tgactcgcgc
     2821 ctgtaccgtc agcgaagcga caagctggag caggtcacga tggatcactc gctgctgcag
     2881 gagctcgtgg atcgtggttt ttattcagcc gaggaggcgc agcgagcagc gaacaagaat
     2941 tacgtcaccc gggccctggg agtggagccc aacgtggacg tggagattca agaggtcccc
     3001 gtgcagaagg gcgatgctta catgctctgc tcggacggac tgtcggatat ggtggaagac
     3061 gaagatattc atttaaccat aaacaccttt ggtgataatc tggataccgt cgccaagcaa
     3121 ttgattcaac tggcgaacga caacgggggc cgcgacaacg tttcggtcgt gatggcgcag
     3181 atcatcgagg cgtttccggc gcgtacgcgc attttcgaca ggatcctggg ctggttcggc
     3241 tgacagaggc acgcacatgg caaggttggt cttgagcctg gatggccagg tgatggcgga
     3301 atacaacatg aacaaggagc ggtacacgat tggccgcctc ccggacaatg acatccgcat
     3361 cgacaatgcc gccgtgagcg gccatcactc gctgatcatc aatatcctga acgactcgtt
     3421 cctcgaggac ctgaacagca ccaacggcac ctatgtcaac ggcaagctga tcaagaagca
     3481 cgcactgcag cacggagacg tcgtcaccgt cggacatcac caactgcgct tcgtggaaga
     3541 cgacgaggct caagacgaat tcgaaaagac gatggtcatc cagccctctt cgcggccggt
     3601 cgaaaaagtc cgtgcggcga acgaagccgc cgagcaggcc tcgccctctc tatccgccac
     3661 gggcagatcg cgcgcgctga gcgatggggc cgcgctcaag aaggccaaac tgcaggtgct
     3721 ctcaggcgcc ttcgcaggcc gcgagctcga gctcaataag gcgctgacca cactgggccg
     3781 ccccggtgta caggttgccg ccattacccg ccgggccgag ggctattaca tcgtccatgt
     3841 ggacagcggc aaggagaacg atttcccgct gctgaacggc acggcgatcg gggcgcaggc
     3901 cacccgactt gccgacaacg atgtgattca gcttgcgggc gtgaagatgg gattcttcgt
     3961 caactgagct ccagcggcac gcgttgactg accccgcata gcagctcgta agggatcgtg
     4021 cccgcgtggc gggcgatctc ctccaccggc aggccattgc cccacagcat cacacgcgtg
     4081 ccgacgtaga cgcctccaag atcggtcaca tccacggcga tcatgtccat ggagactcgt
     4141 ccagcgatcg gtgcgcggct gccgtcgatc agaaccgggg ttccgccggg cagattgcgg
     4201 gcgaggccat cgccgtaacc ggcagcgatg atcgcgatca cggagtcacg ctgggctcgc
     4261 cagcttccgc catatccgac cgtctcaccc cgaacgacct tgcgggttgc aatcacgcag
     4321 gtctccaggc tcatgaccgg ctgcaagccg agatccagcc cgcagccctt gggataaggc
     4381 gagcctccat agagtgcgag cccgggtcgc acccaggcgc aaccgagcgg gaccgagccg
     4441 aagaggccgg ccgagttggc aatgctggtg gcgaagtcga ggtggccaac cgcgctctgg
     4501 aagcgctgca gctgctcgcg ggtcatcaca tcatcgcgtt catcggcacg ggcgagatgg
     4561 gtcagcaagc gaatttccag aggcgccggc tgcagacgcc gcacacgctc gagcgccgcg
     4621 ggaaactcct ccgggcggaa accgagccgg ttcatgccgg tgtcgatctt gatccacagc
     4681 aggaagcgat gcgcgccgcc gaattcctcc agcagctcga tttgcagagg atcgtgcacg
     4741 accaggtcaa agccgtgacg tgccgcctcg aggaggtgct ctcgtgcgaa cacgccctcc
     4801 agcagcacga tcggctgggt gatgcccgcg ccccgaagcg ccaagccctc ttccagcctc
     4861 gccacggcaa acgcatccgc ctcggcaaga gccagcgagg tggggaccag cccgtgtcca
     4921 taagcattgg ccttgaccac ggccatcacc cgggcgccgc gcgcgcgctc gcggattgtc
     4981 ctcaggttat gtcgtagcgc atgggcgtcg atcaccgccc ggatcagccg actcaccgca
     5041 gcacgccttc cgcgtaagca tcgggcatgt ggttctcgaa gcgtgagaat tcgccccgga
     5101 aggtcagcat gatctgcccg gtctcgccgt tgcggtgttt ggcgaggtcg atctccgcga
     5161 ttccccgctt ggtcgtattc ttgtcataga cctcctcgcg gtaaatcatc aggatcatat
     5221 cggcgtcctg ctccagggca ccggattcac gcagatccga catcacggga cgccgatcct
     5281 gccgctgctc gacgccgcga ttgagctgcg acagcgcaat aaccgggacc gccagctcct
     5341 tggccagggc tttcaatccc cgcgagatct cggcgatctc cgtggcgcga ttctccttgg
     5401 tcccggggac ctgcatgagc tgcaggtagt caaccacgac cagatccaga ccgtgctcgc
     5461 gcttgaggcg ccgcgcccgc gcccgcagct cggtgggcgt cagggcgggg gtctcgtcga
     5521 tgaagatgcg cgcattgccg agctgctgtg tagcgcccgt gaggcgagtc cagtcatccg
     5581 gggacatgcg accggcgcgg atgctcccga gcggcacgcg cgcaagcgag gacatcatgc
     5641 gcatcagaag ctgctcggac ggcatctcca tgctgaagat gaccacgctc gcagccgtct
     5701 tcacatcgag cgcggcattc tccgccatgt tgactgccag agtcgtcttg cccatcgacg
     5761 gccgtcccgc cacgatcacc aggtcaccgg ggcgcagccc gccggtcttg cgatcgaagt
     5821 cgatgaaacc ggtcggcaga cctcgcatgc cctcagggtt ctggttccac tcatcgatgc
     5881 ggtcgatcag gggcggcagc agaacgcgtg cggccatcgt ctgactggcg ccacgaaagc
     5941 cactctccgc aatctcgaac accttctgtt cggctcggtc cacgagctcg cgcgcggtct
     6001 gtccatcgtt gttgaacacg gaggaagcga tttcggtgcc cgcgtggacg agctggcgca
     6061 agacggagcg ctcgcgaacg atgtcggcat acgcccgaac gtttgcggcg gtcggtgtgt
     6121 cgcgcgcgag agagccgaga taggccagcc cgccggctcc ttccagcttc gccgtgcgct
     6181 cgagctcgcc caccacggtc acgacgtcac acggcttgcc gccggccacg agcgagccca
     6241 tggcctcgaa gatcatccgg tggtcggagc gatagaagtc gccggagatg acgacgtcgg
     6301 caacattgtc ccaggcggct gggtcgagca gcaacccgcc gagcaccgcc tgctcggcct
     6361 cgaccgagtg tggagggacc ggcgcatccg ccaacgtcag tccgccggta tcgcgacgca
     6421 ccttggcggg accggccact gcgcgagccc cctcggctta ttcttcgggg acgatcgaga
     6481 ccgcgagcgg cacgtccacg tcagcgtgca gatgcaacgt gatcgcgtgt tcgcctaagg
     6541 tgcgcaacgc cccgttcggc agacgcacct cgcttcgctg caccttgaac cccgcgcgac
     6601 tgatggcttc cgcgatatca ctggtgccga tcgagccgaa gagctttccc tcggtccccg
     6661 ccttggccgg gatcaggagc ttgaagtcct tgagcgccgt cgcccgctcc tctgcggaag
     6721 acaggtgctc gcgggcggca acctcgagct ccgcccggcg cgcctcgaag cgcgccacat
     6781 tggcgggcgt cgcgagggtc gccttcccct gaggcagcag gaaattgcgg ccgaagccgg
     6841 acctcacctt gacgcgatcg ccgatgttgc cgaggttggc gaccttctgc agcagaatga
     6901 cttccatggc ttcgcctcag tgctgatccg tgtagggcag cagcgcgagg aaacgggcgc
     6961 gcttgatcgc aaccgcgagc tgtcgctgat agaacgactt ggtgcccgtg atgcggctgg
     7021 gaacgatctt gccggtctcg gtgacgtaac ccttcaacgt cgcgagatcc ttgtaatcga
     7081 tgtacttcac gccttcggcc gtgaagcggc agaacttctt gcggcgtgag aaacgtccac
     7141 cgccaccgcc gccgccgccg ccgccgcccc cgctcccgcc ggagcgctct ttgctcattg
     7201 ccattacaga tatcctcgaa tgctatgccg atgcaggggg cgctcaggcg ctctcgcccg
     7261 ccgggctatc ctcatcgtca gccgacgagg cagaactctc ttcacgcgag cgatcgcggc
     7321 gcgggcgatc gtcatcgcca ccgtcctcat cccggcgctc gcgccgccgg ctgccgtcac
     7381 cctcctcgtc cgcggccttg gccatcggcg agggctcggt gaccgcacta tccatgttga
     7441 ccaccagatg tcgcagcacc gcatcgctga aacggaacgc gccggtcaga tcatcgagcg
     7501 tcttctgatc gcactcgatg ttcaccagca catagtgcgc cttgtggacc ttggcgatcg
     7561 gataggtgag ctggcggcgg ccccagtctt ccaggcggtg cacgtggccg ccgcctgcgg
     7621 caatcatgcc cttgtagcgc tcgatcatgg cgggcacctg ctcgctctga tcgggatgca
     7681 cgagaaagac gatctcgtaa tgcctcatga tgactcctta cggatagtgg ccggcaccag
     7741 cccgacttgc atcgaggctt tcgccagcgg gcagccgtcc atcgcgaggt cagcgaggtg
     7801 cggcaaggag aacgccgggt atccggcgca gggccgcgaa ggatacacaa tcaggataac
     7861 cgctgccagc acagcccgac ttacccgctg cgagcgcagc ccgggttaac cgctgccccg
     7921 ctgccgtacg gcctcgtaga gcagcatgcc ggcggccacc gagacattca ggctctcgac
     7981 cgcccccagc cggggcagcc ggaccagcca gtcgcagctc tggcgggtca gatgcctcaa
     8041 gccggcccct tccgccccga gcaccagcac gatgcccgtt ttcagatcca cttcgtgggg
     8101 cagcttgtcg ccctcggaat cggccccggc cacccaaaga cccgcctgct tgagcaactt
     8161 cagcgtgcgg accaggttgg tgaccgccac gaccggcgtg gtctcagcgg caccgacggc
     8221 gacctttcgg acgaccggag tcagttgtgc cgcgcgatcc cgcggaacga tgaccgccag
     8281 ggcgccgcag gcatcggcgg tacgcaggca ggcgccgaga ttgtgcggat cctgaacacc
     8341 atcgagcgcc accaggagcg gatcccgcac cctgcccaga gcatcgagca gttgatcctc
     8401 ggaccaggga ggcagaggtg agatttcggc cgccactcct tggtgagcca cgtccccgag
     8461 ctgctgctgc agcgcgcgcg catcaacccg cctcacgggc cggctctggt ttcgggcgat
     8521 ctcttcgatc tcgcgcaccc gggggtcatc gcgccgatcc gtcagcatca cgacaacgac
     8581 ctgctcgggc cgtctctcga gaatcatccg gacaggatgc acaccgtaga tcgtctcggt
     8641 cgtcttcaca cgagctcagc cgacatcatc gacccactcc agatccacca ggccctcgat
     8701 cggattgacg gcggtcacca cgacgcggac ggagtcgccg gttcgcagtc tgcgcctggt
     8761 ccgtttgccg atccaggcat gcccgttgtc ctccatgacg taatcgtcgt cgcgcagatt
     8821 gtcgatatgc agcaggccgt ccactgccac agccagtatc tgcacgaagc agccgaactc
     8881 aacgaccgtc gtgatgatcc cgtggaaggt ctgaccgatc cgctcccgca gataggtgca
     8941 tttgaggaag gtggccacgt atcggtccgc ttcatccgcg cgtttctcga gtcgggatgt
     9001 cgcctctccg agcgtggcga gctcaccgct gtcgtaatgc actgctgccg gtccacgcgc
     9061 gccgatcaaa gccttgagag tccgatgcac cacgagatcc gggtagcgcc ggatcggaga
     9121 ggtgaaatgg gcatagtgcg acagcgcaag cccgaaatgc ccgatgttgg tctgctgata
     9181 cacggcttgc ggcatggccc gcacgatcag ggactcgatg aacggccgcg cttccgaggt
     9241 ccccaaccgc cgagtgatcg cctgcaggtc gcgagtcgtg ggattctcgg gaatctgggc
     9301 ctcaatgccc agtgcatgca gcgttgaatg cagccgttcg agcttctgct cttcgggctg
     9361 cgcatgcacc cgatacagtg agggcgtatg cgtcctgctc agctcctgag cgaccgcaac
     9421 gttggcggcg atcatgcatt cctcgatcag ccggtgcgca tcattgcgcg agcgcagctc
     9481 gatggcgcgg atgcgctccg ccgaatcgat cacgaactct gcctccgcag cgtcgaagtc
     9541 cagagctccg cgccgattgc gtgccttctg cagcgctcgg tagacgtcca ccagaatcaa
     9601 cagccgatcc gacaacggac ccagtgccga ccgtgccgca gggcggcctt cgaagagaac
     9661 ctcgtgagcc tgcgtgtacg tcagtcttgc ggccgagcgc atcacggctg gatagaaccg
     9721 tgaatgcttc agcgtgccgc tcctggtgat gaccatgtcg gccgcgaagc agagccgatc
     9781 gacatgaggt gcgagggagc acagatgatc cgacagcgcc gtcggcaaca ttggcagcac
     9841 ccgggtcggg aaatagaccg aggtaccgcg ctcgcgggct tcgacgtcga gcgccgtgcc
     9901 cggtcgcacg tagtggctca catccgctat cgcgacaatg agccggaacc cttcgggatg
     9961 cggctccgca taaaccgcat cgtcaaaatc ccgagcatgc tcgccgtcga tggtcaccag
    10021 tggcaagctg cgcaaatcct ctctcgcgcg cgcttcggcc ggatccacgt gctcgcccca
    10081 ggcctgtccc tcgcgcagcg ccgcggaaga gaactcgtgt ggcaggtcaa agcgtgcgat
    10141 tgccgattcg gtggcgagct cgaccggtcg gtccggatcg agtcgcttca ccactgccgc
    10201 atgggccggt gcggttgagg tcgcatgccg aatgatgcgt gcgatgaccc agtcaccgct
    10261 gcgagcgccg ccgagctcgg ctggggagac agcacagtgg agctgcagac ggcgatcggc
    10321 tgcgttgatc cagggggtcc ggccttgcag ctcaacagtc cccaggaagc tattcacgcc
    10381 gcgcgtcaag attttttcaa ccgtcccgag atagcggttg ctcgcatcgc gggcgagctt
    10441 caccctgagc cggtcaccat gcatgacgcc gcgcatctcc ccgggcggga tgaagacgct
    10501 gtccttcatg ccttcgacgc gcaagaagcc gtagcccgca cgatttgcgc tcacgacgcc
    10561 ttcaacgacg ccggccatcg aagcgccgtc gtccgcaccc gtgcgacgtt tgttgtgcgt
    10621 tgggtggcga agggtccgat cctggcgtgc gctgccggat gcgtgagcac ggcccgactg
    10681 ggtagcacgc gaaccccggg tgccggtacc gaatttcccg cggcgcgctt tcaattgaca
    10741 gccctcgacc ccgtgcgtac tgttcggcgc cttccatcgc caatgctttg gcgagcccgg
    10801 gtggcggaat tggtagacgc actagtttca ggtactagcg ggtaacaccg tggaggttcg
    10861 agtcctctcc cgggcaccat aatttcctta aaaatcaaaa taatagatcc aatagttaga
    10921 gttgagatca ggatgcttga gcaggcggaa tagccagctc ggggcgggat tgttaatctt
    10981 ttcgcggccg catcctagca ccggtgatgg ggccaagcgc cgcaattgca agatgcacgg
    11041 cccagcgccg atcgtgcaaa gatccattcc gcgagtgagt caacgttgcc atcgagtgcc
    11101 aaatcggcag cccgattcgc gcttcccgtc cagatagtgg cgtgtcgcaa accaacgctc
    11161 gccggaaacg ctactgagct actgaagcgg gtgtcttctt aagtagctct gcatttgccg
    11221 cgcgcgctgc agatgcctgt cggcgcgcct aaggtatttt cgtgattcgc ccagaatctg
    11281 gcgaatctgc gcgcgcaagt ctacgcacga caagcaaccc gtgcgcgctg tggtaaacga
    11341 cctgcccagc cagaaccaca ccggcgcgta attccaaaat tgctgctcca gcatagccgc
    11401 cgcgttggtc tttgatcggc acgcaatgtc cccgcaagca ttgaggcaca atcgcacatc
    11461 atgcgtccaa gccggcacct tccgaacctg cttgtacttt gcgcagcgct gtcgcttgca
    11521 tcgccgctcg cggcgtacgc cgggggctcc gcccgtcaaa gcgtggcggg ctccgcgggc
    11581 cttgtcgccg acatcgattc agactcgggt cgctatgagg taagcagtaa agaggcgggc
    11641 tggacgttcg ccgggaacct cggcggcgca gtttcggccg tcgtggcaaa ggacggacgg
    11701 gatccgctcg gagcgttccg agagttgagc tttcgctgga aacatccggc ggctctcagg
    11761 ggcagcatcc gcgcctacgc cgaccgcccg gttatgctgt tcaccctctc caccgaccaa
    11821 gcggtttccg acgccgcggt cgtgcgtttc cccgacttca gcgaagcgcc gaggaacctg
    11881 caccacttca gctatgcaaa cgatgcgttc gctccagcga gttttgcgct cgagcaaacc
    11941 ggcacgccgt ggctgctcta cgacgagcac gcacacgccg cggtgctgtc accggccgcg
    12001 aatttcatga tcgcgagcat gtggagcaac gacgggacgg aaatcgccag tggcctcaac
    12061 cgctcggtcg catcgctgcc ggccggcttc aggcacatga cgctcatggt catcggcccg
    12121 ggagtgaatg cgacctggga cgcatggggc agcgcgctgt gcgcactcct aggcgcgcat
    12181 cgaccggtga acgatgccga cgccggcttg cgctacctcg ggtactggac cgaccacggc
    12241 gcacagtact attacgacta cgaccctcgg ctcggttacg ccgggacgct cgaggcgctg
    12301 gtgcggcgct accgcgatga ggcgattccc attcgctatc tgcagctcga cagctggtgg
    12361 tactacaaga gtctgacgga tccaaggggc aaaacgggac gcccgatgaa tcccgcgtta
    12421 cccgcggggg aatggaatcg ctacggcggc ctgctcaagt accaagcgca tccggcgctc
    12481 ttcctggatg gtctggcggg ctttcagcag aaggtcggtc tgccactcat cacacacaac
    12541 cgctgggtcg atccggctag cccctatcac gagcgctacc gcatctcggg attcgccccg
    12601 gtcgatctcg cgtggtggaa ggagatcatc ggttacctcg cttccgccgg cgtagtgacg
    12661 tacgagcagg actggctcaa cgtcatctac gaacattcac ccgaactttt ctcgagcccc
    12721 gcgacgaacg ctgcctttac cgacggcatg gcgaacgcgg cgcgggagcg cggactgacc
    12781 cttcaatact ccatggcgct gccgcggaat tttctccagg gcgcccgcta tgagaacctc
    12841 accaccattc gggtgagcgg cgaccggctc acgcgtgata agtgggatgc ggctctttac
    12901 acctcgcgcc tcgccagcgc ccttgggatc tggccgtggg tcgatgtgtt tatgagcagc
    12961 gaggcggaca acctcctgtt cgcgacactg tcggcgggca tgatcgggat cggtgaccgg
    13021 atcggcgctg agaaccggga aaatctgctt cgcgccgtgc ggctcgatgg ggtgattatc
    13081 aagccggata cgccgctcgt tccgcttgac gccatgtaca tgccctcctc gcggcgtatg
    13141 ccgatgatcg cggcggctca tagcgagcac ggtgcgctac atagcagcta cgtcttctgc
    13201 tacagccgcg gatcgagggc cgcgagcact tcgttcacac cggcacaggt gggggtgtcc
    13261 cgtcaagcgt atgtgtatga catgcgaacc cgcaccgcaa gacttttgca gccgtcggag
    13321 gtcttcgcct tgacacttgc tcccgatgaa actcggtatt tcgtcgtggt accgctcacg
    13381 cgcgcgggaa tcagtttgtt cggtgacgag cacaagttcg tcccggacgg ccgcaagcgt
    13441 atcgctgcgc tcgaggacga ggcgaagctg cttactgcca acgtgacgtt cgcagcgcag
    13501 gaaccatcgg tgcggctctt tggttacgcg ccgcggcgac cgacggtgac ggcgcagacg
    13561 ggctctgtgg gtgaggtcga cttcgatccg agtagcgggc gctttgaagt cacggtgtcg
    13621 ccgggtccgg ggtcgcaact cgagggtccc gccgatgatc cggtgcgccg agctgtgatc
    13681 gccctgcgcc tggatcggtg acccaagagc gaccaggctg tcccggcacg gcgctccgat
    13741 cgacgccgaa atgacgaatc agatcgacgc cctg
//
LOCUS       DASRHN010000343         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1446357, whole genome shotgun sequence.
ACCESSION   DASRHN010000343 DASRHN010000000
VERSION     DASRHN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446357"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFB37_13780"
     CDS             complement(<1..377)
                     /locus_tag="VFB37_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZO23567.1"
                     /translation="MIPIALLLLAMASFQSGASIAKTMFPVVGAAGMVTLRIGFGTLI
                     LALAMRPWRARITTANWRPLLGYGISLGVMNLLFYCALARIPLGIAVALEFTGPLAVA
                     VLSSHRAVDFAWVALACAGLLLLL"
     gene            422..1342
                     /locus_tag="VFB37_13785"
     CDS             422..1342
                     /locus_tag="VFB37_13785"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZO23568.1"
                     /translation="MPDAGAEVFRSPRRVECDERAFVLAAVGIASEITAQGEDFLLEV
                     SPPDLDQALTHLQQYDLEAQRRSAAPLIPPPQLHPGAWTGSAVYAAVLLTVAYAVANG
                     IWRLDAFDVGDLDATRVQSGQWWRVWTALTLHLDGPHLAANTVAGIWFGYLAGRLIGA
                     GNAWLLVLLGAGAANWIEAHFAPEGYRSVGASTAVFTALGLLSAYSWSTRFRLPQRWA
                     ARWGPLVAGIALLGWTGTGGAEATGPRGADGAEVDVVAHALGFAIGLLPGAAAAWRPV
                     ARVLGRLPQWVMGLAALALLAWAWWRALSS"
     gene            complement(1388..1888)
                     /gene="sixA"
                     /locus_tag="VFB37_13790"
     CDS             complement(1388..1888)
                     /gene="sixA"
                     /locus_tag="VFB37_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZO23569.1"
                     /translation="MELLIVRHAIAFERDRRRWPEDSERPLSPAGIRRARRAAAGLKE
                     LIAAPERVLTSPLLRARQTARVLSEFAGWPQALHCPELSPGQAPAAVLRQLRQHREKR
                     IALVGHEPGLGLLVAACLLDEPGHLGIELKKNAVACVSFEGAPRAGAGTLRWLAPPRF
                     LRSARK"
     gene            complement(1923..>2276)
                     /locus_tag="VFB37_13795"
     CDS             complement(1923..>2276)
                     /locus_tag="VFB37_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23570.1"
                     /translation="EMLRALRRWQETLGVQHDAHVASMRLQGLAAAPPKSLRPETLFL
                     MGRLAEHYAVAAGKARKRFAKSYRKVRGRWKILAQKSPLLQTAAAPAPARAQPAAPAA
                     PLNEPPPQASSNPSG"
BASE COUNT          379 a          732 c          793 g          372 t
ORIGIN      
        1 agcagcagca gcaatccagc gcaggcgagc gcgacccagg caaagtccac ggcgcggtgc
       61 gaggacagca cggcgactgc gagtggaccg gtgaactcca gagcgaccgc gatgccgagc
      121 ggaatgcggg cgagcgcgca gtagaagagc aggttcatca ccccgagcga gatgccatag
      181 ccgagtaatg gccgccagtt ggcggtcgtt atccgggctc gccacgggcg catcgcgagc
      241 gccaggatca gggtgccgaa gccgatgcgc agcgtgacca tccccgctgc gcccaccacg
      301 ggaaacatcg tcttggcgat cgatgccccc gactgaaacg aggccatcgc cagcagcagc
      361 aaggcgatcg gaatcactgt cgagactcgc ctgttaagct gcgccacgaa ggaattaaat
      421 catgcccgat gccggtgctg aagttttccg ctcgccgcgt cgcgtggaat gcgacgagcg
      481 ggccttcgtg ctcgcagcgg tcggtatcgc cagtgagatc acggcgcaag gcgaagattt
      541 tctcctcgag gtttcccctc ccgacctcga tcaggcgctg acacacctgc agcagtacga
      601 tctggaggcg cagcgcagat cagcggcgcc gctcatcccg ccaccgcagc tgcatcccgg
      661 agcctggacg ggaagtgcgg tgtatgcagc agttctgctg acagtggctt acgccgtggc
      721 caatggcatc tggcgactgg atgcgttcga tgtgggcgat ctcgatgcga cgcgcgtgca
      781 gagcggtcaa tggtggcggg tctggaccgc gctgacgctg catcttgatg gtccgcacct
      841 ggctgcgaac accgtcgcag ggatctggtt cggctatctg gccggacgtc tgatcggcgc
      901 gggcaatgcg tggctgctcg tgctgctcgg agcgggcgcg gccaactgga tcgaagcgca
      961 cttcgctcct gagggctacc gctcggtggg cgcctcgacg gccgtcttta ccgctctggg
     1021 gctcttgtcg gcctactcgt ggagtactcg gtttcgcttg cctcagcgct gggccgcgcg
     1081 ctggggaccg ctcgtcgcgg gcatagctct gctcggttgg acggggaccg gcggcgcaga
     1141 agcgaccggc ccgcgagggg ccgacggcgc ggaggtggat gttgtcgcgc atgccctcgg
     1201 ctttgcgatc ggccttcttc ccggagcggc cgcggcctgg cggccggtcg cgcgcgtgtt
     1261 aggacggctg cctcaatggg tcatggggtt agcggccctg gcactgctcg cttgggcctg
     1321 gtggcgcgct ctctccagct agttcatcac gcgcagtgcc gcagctgacg tacgtgatgg
     1381 acaagctcta cttgcgagcg ctgcgaagaa aacggggcgg agccagccat ctgagcgtgc
     1441 ccgcgccggc tcgcggggca ccctcgaagc tcacacacgc cacggcattc ttttttaact
     1501 cgatgccgag atggcccggc tcatcgagca ggcaggcggc caccaacaac cccaagccgg
     1561 gctcatggcc cacgagcgcg atgcgctttt cacgatgctg gcgcagctgg cgcaacacgg
     1621 cggccggcgc ttgtccgggg gaaagctccg ggcaatgcag agcctggggc cagccagcaa
     1681 actcgctgag gacgcgggcg gtctgcctgg cacgcaaaag aggactggtg agaacgcgct
     1741 cgggcgcggc gatgagctcc ttcaggccgg ctgcggcccg gcgagcacga cggatgccgg
     1801 ccggagataa cggtcgttcg gaatcctcgg gccagcggcg tcgatcgcgc tcgaaggcga
     1861 tggcatgacg aacgatcaac agctccatga ctttgaacgg ccgctctgct ctgacggcgg
     1921 cttcacccgg acgggttgga tgaagcttgc ggcggcggct cgttgagcgg cgccgccggg
     1981 gccgcaggct gagctctggc cggagccgga gcagcggctg tctgcaggag cggcgacttc
     2041 tgcgccagga tcttccagcg gccacgaacc tttcgatacg acttggcgaa gcgcttgcgg
     2101 gctttgcccg cagcgacggc gtaatgctcg gcgagccgcc ccatcaggaa gagagtttcg
     2161 ggccgcaggc tcttgggcgg tgcagccgcc aacccctgca ggcgcatgct cgccacatgc
     2221 gcatcatgct gtacgcccag agtctcctgc catcgccgca gcgcacgcag catctc
//
LOCUS       DASRHN010000344         5130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1937651, whole genome shotgun sequence.
ACCESSION   DASRHN010000344 DASRHN010000000
VERSION     DASRHN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937651"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFB37_13800"
     CDS             <1..487
                     /locus_tag="VFB37_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HZO23571.1"
                     /translation="HALGRHYPGGLPAFVQAMNGKARSLGMTHSRFVDPAGLSSENVA
                     SAEDLSRLVEAASQNPIIREYSTSPDYAVAVGRRLLEFRNTNALVSNPSWNIIVQKTG
                     YISEAGRCLVMQAVIEGRTVVIVLLNSVGKYTRVADAVRIRRWMEARLNAPTRVVADK
                     A"
     gene            complement(484..1230)
                     /locus_tag="VFB37_13805"
     CDS             complement(484..1230)
                     /locus_tag="VFB37_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23572.1"
                     /translation="MLANLKVWLDHFEYHAQHPRRLPEAISDYLAPEERDLIARSIAT
                     FQLGEQSAGTHFLAAAYRFALQHDAAPVARATELLLREEQQHARLLGRFMEEHAIATR
                     SHDWTDWIFRRLRRLGGFELYLCVLLSAELIGTVYYRSLEAATRCQRLRLLCRVLVAD
                     ELAHIGFESDLLLAMRGRHSTALRVLLERLHEIVFLVTGIVVWTTHRSVLSRGGYGVF
                     SFLKACRAQFSFYLATGSQPRARADAARRT"
     gene            1487..2269
                     /locus_tag="VFB37_13810"
     CDS             1487..2269
                     /locus_tag="VFB37_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23573.1"
                     /translation="MFRSAFLLAAGLCTAAVLGAGPVVAAEHIPAYVSKAVADPSRPA
                     EDRQRDAERKPAETVAFAGIKPGDRIIELVPSKGYFTRILSKVVGPKGHVYAISPPRR
                     ANARADAPDPVAATKALAAEPGYSNVTAEVRTPEAFSAPESEPVNVVWTSQNYHDFHN
                     VPNLDIAAFNKAVFNALKPGGLYIVLDHAAAAGSGTRDTSTLHRIDPEAVKTEVRAAG
                     FDLAGESKLLHNPQDDHTLKVFDPQIRGKTDQFILKFRKPKK"
     gene            complement(2274..2966)
                     /locus_tag="VFB37_13815"
     CDS             complement(2274..2966)
                     /locus_tag="VFB37_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO23574.1"
                     /translation="MTDLPYRTALIVGAGTGISASVARALSTAGLKVALASRTPRKLD
                     TFAAEIGALTFAVDASEPSAVEQLFTEVDVRLGEPEVVLYNVSTRLRGPIVGLDPDEV
                     ARALATSALGGFLVLQQAARRMLARGRGAILITGASASVKGFAQSAPFAMGKFALRGL
                     AQSAARELGPHGIHVAHFLIDGSVRSATRPDSQDRTLDPDAIAQTYLDVLRQPRNAWS
                     FEVDLRPWAEPF"
     gene            complement(2963..3265)
                     /locus_tag="VFB37_13820"
     CDS             complement(2963..3265)
                     /locus_tag="VFB37_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23575.1"
                     /translation="MTEQMRSGSPELRAYQVMDWIAPDDRTLVVNGVDRSLFEGRFSR
                     GCAGLRLANSIAFIVPPGAALSGYSGVVLPDGTRCRFARFTRLSTTAPAAKAAENP"
     gene            3553..4305
                     /locus_tag="VFB37_13825"
     CDS             3553..4305
                     /locus_tag="VFB37_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZO23576.1"
                     /translation="MNTLTSKLRGGSVGLAIVIACATALPAHADEKDSGRKTASRQET
                     IGVATGFALGAVTAGPIGAIVGAATGAWLGDRDHKLAVQRSVLAGDLDRTQGEQARLA
                     RNVTELNGSLAREQEHSEQLDLALGHTDEIELDIGFRTNDDSIQVQSMSPLLKLGSLA
                     AAMPDARVRVSGYADARGSDELNESLSRRRAEAVAAVLESAGMSRERLLVEAHGSSAA
                     TSAEGDLDGYAFDRRVTVRIEKSQDGAVARND"
     gene            4375..4740
                     /locus_tag="VFB37_13830"
     CDS             4375..4740
                     /locus_tag="VFB37_13830"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO23577.1"
                     /translation="MTCSIVALDGSIRPEISQPAPDRQVSGNPSTQISNYFSDASGQF
                     FAGHWSASRGKWRIRYTENELCVMTSGKVSIESETGERCTFVAGDAFVVPAGFCGTWE
                     VLEDCSKVYAVFEPRAPGS"
     gene            4867..5019
                     /locus_tag="VFB37_13835"
     CDS             4867..5019
                     /locus_tag="VFB37_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23578.1"
                     /translation="METVTITASRGFTMSDMEMLVGSAVTIAALAWIVLSRLKHLHNR
                     HQEPHV"
BASE COUNT          943 a         1686 c         1670 g          831 t
ORIGIN      
        1 ccatgcgctc gggcgccact accccggcgg acttccggcc ttcgtgcagg ccatgaatgg
       61 caaggcccga tcgctcggga tgacccattc ccggttcgtg gacccggccg gcctctcgag
      121 tgagaacgtg gcgagcgccg aggatctgtc ccggctggtc gaggcggcgt cgcagaatcc
      181 gatcatccgg gagtactcca cctctccgga ctatgcggtc gcggtcggcc ggcgcctgct
      241 cgagtttcgc aacaccaacg ctctggtctc gaatccgagc tggaacatca tcgtgcagaa
      301 gaccggctat atcagcgaag cgggccgctg cctcgtgatg caggcagtca tcgaaggccg
      361 cacggtggtg atcgtgctgc tcaactcggt gggcaagtac acacgagtag cggatgccgt
      421 acggatccgg cgctggatgg aggccaggct gaacgcgccc acccgcgtcg tggcggacaa
      481 ggcctaagtt ctccgggctg catccgctct ggctctgggt tgtgagcccg ttgccagata
      541 gaacgagaac tgggcgcggc acgccttcag aaacgagaac actccgtagc ccccacggct
      601 gagaacgctg cggtgcgtgg tccagaccac gattcccgtc accaggaaca cgatctcgtg
      661 gagccgctcg agcaacaccc gcagggccgt tgaatggcgg ccccgcatgg ccagtagcag
      721 atccgactcg aagccgatgt gcgcgagctc atcggcgacc agtacgcggc agagcagccg
      781 caaacgctgg cagcgggtgg cggcttcgag cgagcggtaa tagaccgttc cgatcagctc
      841 ggcgctgagc agcacgcaca gataaagctc gaacccgccg agccgtcgca gccgtcgaaa
      901 tatccagtcc gtccaatcgt gcgatcgcgt ggcaatcgca tgctcctcca tgaagcgacc
      961 caggagcctc gcgtgttgct gctcctcgcg gagcagcaac tcggtcgcgc gggcgaccgg
     1021 ggcggcatcg tgctgaagcg caaagcgata ggcagcagcc aggaaatgag tgcctgccga
     1081 ctgctctccc agctggaacg tagcgatgga gcgtgcgatc agatcgcgct cctcgggcgc
     1141 caggtaatca gagattgctt ccggcaagcg ccgtggatgc tgcgcgtggt actcgaagtg
     1201 atccagccag accttgagat tggcgagcat ctagatttcc tccaggcatt cccgatccca
     1261 tcaggctgga ggagaattcg gctggaccgc tgcggcaatt cgggcaaatt cgggcagtcc
     1321 cggcccgtcc ccggctctag cgcttgccga gggtggccga ggcacagctc cggcatgcgt
     1381 gtggccgcgg ctacatcacg ggtgcgggtg cgcgttccgc ggtcgtcgcc gtcgcaaacg
     1441 aactggtaaa gttccccgct caccccgcca tcacgagagg ctactcatgt ttcgcagtgc
     1501 ttttctgctg gccgcaggcc tgtgcaccgc ggccgttctg ggagccggcc cggtcgtggc
     1561 cgccgagcat atccctgcct acgtcagcaa agcggtcgcg gacccgagca gacccgctga
     1621 ggaccgtcag cgcgatgcag agcgcaagcc ggcggagacg gtcgcgttcg ccgggatcaa
     1681 gccgggggat cgcatcatcg agctggttcc ctccaagggg tacttcacgc gcatcctgag
     1741 caaggtcgtc ggacccaagg gccatgtgta tgcgatctcg ccgccgcgtc gcgcgaacgc
     1801 gcgcgcggat gcgcccgatc cggtggccgc aaccaaggcg ctcgccgccg agcccggcta
     1861 ctcgaacgtg accgccgagg tgcgcactcc tgaagccttc tcggccccgg aatccgagcc
     1921 cgtcaacgtg gtctggacat cccagaacta ccacgatttc cacaatgtgc cgaacctcga
     1981 catcgcagca ttcaacaagg ccgtcttcaa cgcgctcaag cctggtggtc tgtacatcgt
     2041 gctcgaccac gcagccgcag cgggctccgg gacgcgcgac acctcgacgc tgcatcggat
     2101 cgatcccgag gccgtcaaga cggaagtacg ggccgcgggg ttcgatctgg cgggcgaaag
     2161 caagctgctg cataacccac aggacgatca cacgctcaag gtgtttgatc cgcagatccg
     2221 cggcaagacc gaccagttca tcctgaagtt ccgcaagccg aagaaatagc ggctcagaac
     2281 ggctcggccc agggcctcag gtccacctcg aacgaccagg cgttacgagg ctggcgcagc
     2341 acgtccagat aggtctgcgc gatggcatcg ggatcgagtg tccggtcttg cgagtcgggt
     2401 cgcgtcgcgc tgcgtaccga gccgtcgatc aaaaagtgcg cgacgtggat gccgtgtggt
     2461 cccagctcgc gtgcggcgct ttgtgcgagc ccacgcagcg cgaatttccc catcgcgaac
     2521 ggggcggact gcgcaaaacc cttcacgctg gcgctggcgc ccgtgatcag gattgccccg
     2581 cgaccgcgcg cgagcatccg tctcgcggct tgttgaagca ccagaaagcc cccgagagcc
     2641 gatgtcgcca gcgcgcgcgc gacctcgtcg gggtccagac ccacgatagg cccacgcaga
     2701 cgcgtggaga cgttatagag gacgacctcg ggctctccga gccggacgtc gacctcagtg
     2761 aagagctgct ctacggcgct tggctcggaa gcatccacgg caaatgtcag agcgccgatt
     2821 tcggcagcga aggtgtcgag ctttcgcgga gtgcgcgagg ccagcgcgac cttcagacca
     2881 gccgtactca gagcgcgcgc caccgaggcg ctgatgccgg tcccggcacc gacgatgagg
     2941 gcagtgcggt aaggcaggtc ggtcatgggt tctcagctgc cttggcggcc ggcgccgtcg
     3001 tggaaaggcg cgtgaatctt gcaaaccggc agcgcgtgcc atcgggcagg acaactccgg
     3061 aatagccgct gagcgcagcg cccggaggca cgatgaaggc gatactgttg gcgagccgca
     3121 ggccggcgca tccccggctg aagcgaccct cgaacaggga ccggtccacc ccgttcacca
     3181 ccagggtacg gtcatcgggg gcaatccagt ccattacctg gtaagcgcgc agctcgggac
     3241 tcccggagcg catctgctca gtcactttac cgggcaccgg atccggtccc gaagggctcg
     3301 cggcgcaacc caccagcccg aacggggcga gcgaccccag gccgcccagc cataagagcc
     3361 cggctcgcca cgcgtttctg cggagccccc tgcgccaagc tgagttcgtc attctcggat
     3421 ccgtgccacg attcgccatg agtcttcccc agcctgccgc atttccatca ccgcggccac
     3481 tgcccgctgc ggtgaactgg cagcagctcg gaacacctgt tccgcttcac cacacggtcg
     3541 gagggatgac tcatgaatac gctgacgagc aaattacgcg gaggttcggt cggactggca
     3601 atcgtgatcg cgtgcgcgac cgcgctcccg gcccacgccg acgagaaaga ctcggggagg
     3661 aagacagcct cgcggcaaga gaccatcggg gtagctacgg gctttgcgct gggcgcggtg
     3721 accgccggtc ctatcggggc catcgtgggc gcagccaccg gggcgtggct gggggatcgg
     3781 gatcacaagc tggcggtgca gcgctctgtg ctggccgggg acctggaccg cacccagggc
     3841 gagcaggctc gccttgcccg caacgtgacc gagctcaatg gctcgcttgc gcgcgaacag
     3901 gagcactccg agcagctcga tctggcgctc ggccacaccg atgagatcga gctggacatc
     3961 ggcttccgca ccaatgacga ctccattcag gtgcagtcca tgtccccgct gctcaagctc
     4021 gggtcgctgg ctgctgcgat gccggatgcc cgcgtccggg tttcaggcta tgccgatgct
     4081 cgcggatcgg acgagttgaa cgagagcctg tccagacggc gggccgaggc agttgctgct
     4141 gtgctcgaga gcgcggggat gtcccgggag cgtctgctcg tcgaggcgca cggaagctct
     4201 gcagccacga gtgccgaagg agacctcgat ggttacgcgt tcgatcggcg agtcacggtg
     4261 cgtatcgaga aatcgcagga cggagccgta gctcgcaatg actgaggcga caacgggcca
     4321 accggcgctc gctcatgcgg ggatcgcggt gagattttgc gctacgatcc ccgcatgacc
     4381 tgcagcatcg ttgccctcga tggatccatc aggcccgaga tctcccagcc ggcgcccgac
     4441 cggcaggtca gcggcaatcc ctccacacag atctccaact acttcagcga tgcgagtggg
     4501 caattcttcg ccggccactg gtcggccagc cgcggcaaat ggcgcatccg ctacaccgaa
     4561 aacgagttgt gtgtcatgac ctcgggcaag gtatcgatcg agagcgaaac cggagagcgc
     4621 tgcacgttcg tagccggcga tgctttcgtc gtgcccgccg gcttttgcgg cacgtgggaa
     4681 gttctcgagg attgcagcaa ggtttacgca gtgttcgagc cccgcgcgcc cggatcctga
     4741 gcctgctgaa cataacgccg acggtcggtg aagcctgtcg cagatcaatc ccggtcagcg
     4801 gcagccgcgt gcgattccat ggacactgtc cgggcttgag taggcttgct tgcctggagg
     4861 caccccatgg agaccgtcac catcacggcg tcccgggggt tcacgatgtc ggatatggaa
     4921 atgctcgtgg gaagcgcggt caccatcgcc gctttggcgt ggatcgtgct gtcccggttg
     4981 aagcacctcc acaaccgcca ccaggaacct cacgtctgac gccagtccga gagagcacga
     5041 ggacagctct cgcagccgat gcgcatgccg cgtctcggct gtttctcagg gccggcagcg
     5101 agcccaggcc ccaagcttcc cgcgctccaa
//
LOCUS       DASRHN010000345         8142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2426837, whole genome shotgun sequence.
ACCESSION   DASRHN010000345 DASRHN010000000
VERSION     DASRHN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426837"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="VFB37_13840"
     CDS             complement(<1..1879)
                     /locus_tag="VFB37_13840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO23579.1"
                     /translation="MSQLDLLVPDLGNFADILVVDVLIKTGDKVEVDTPLVTLETDKA
                     SMDVPATSAGTVAEVLLKRGDKVSKGSVIARLQASAQSEPQAAISEAFDAEELERTVR
                     QPVLSASAIAKQKQAEREAPAAQPPSPEDKPSPEDKPSPEGKPSTAPPQAAGSESSKA
                     QAPDGERSTGRPPATDSRPPAAEAQPPATEAERSTAQPGSGSERSTQRKTARSESSVA
                     PRQTEGAQLESAQARADRGARPEPAGDTVRMPMPDLGRRKLGSAEFNRSTQLLVLGSG
                     PGGYTAAFRAADLGMQVTLVERWPVLGGVCLNVGCIPSKALLHAAKVIEDAEAMSANG
                     IAFADPVIDRSKLRGWKNGVVRKLTGGLRMLAKQRKVEVVQGVGRFVSTNVLEVMGSS
                     GSERIHFDACVIAAGSEAIRLPAMPSDPRVMDSSEALELPDFSGGLLVIGGGIIGLEM
                     ACVYEGLGARVSVVELTSQLMPGCDPDLVRPLERRIRGRYEQILLDTKVARVEALQEG
                     LRVSFEGRNAPSPQVFARVLVAVGRVPNGKAIGADAAGIVVSDRGFIPVDKQMRTNVP
                     HIFAIGDIASTPLLAHKAMHEAKVAAEVAAGHKSAFDARVIPSVAYTDPEIAWVGLSE
                     TE"
     gene            complement(1876..3312)
                     /locus_tag="VFB37_13845"
     CDS             complement(1876..3312)
                     /locus_tag="VFB37_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZO23580.1"
                     /translation="MNAAESLVEVRVPDMGNFKDVDIIDVLVKPGESIEPEAPLVTLE
                     TEKATMDVPSTVGGRIEKLHVSKGGKVSTGDLIATVRALTAPGEAASPPSAPAGPKQE
                     SGAASGTAAGAKPSEGTERKADAKEAEREVATQKRDAPSEERAAKHEATRTESASVES
                     PAGTSLPPINEPGFSRAHAGPSVRKFARELGVDLTRVTGTGFKERVTHEDVKGFVKQH
                     LSAGAGAAGAASGALPQVPSVDFAQFGPVETQPLSRIQRISGPRLHASWVNIPHVTQF
                     EEADITALEDARTKLKQKASQEGIRLTSLAFIVRACVKALQEFPTLNSSLDPGGPNLI
                     LKKYLNIGFAADTRHGLVVPVIREADRKDVYEIARHLAELSQKARAGKLTPQQMQGGT
                     FTISSLGSLGGTAFTPIINAPEVAILGVSRATLRPVYRDGELVPRLILPLSLSYDHRV
                     IDGATAVRFTTYLAETLADPRGLLEAVP"
     gene            complement(3323..5992)
                     /gene="aceE"
                     /locus_tag="VFB37_13850"
     CDS             complement(3323..5992)
                     /gene="aceE"
                     /locus_tag="VFB37_13850"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZO23581.1"
                     /translation="MTESPKLDDLDPVETQEWLESIDSVLKTHGAERAHFIIERLIDY
                     TRRSGAYLPFKPNTAYVNSIPTGREPEYPGNRALERRIEAYIRWNAMAMVVQANRVST
                     EFGGHLSSYASSATLYEVGFNHFWRAPSERHPGDLVFVQGHSSPGVYARAYLEGRLAE
                     DQLRHFRQEAGGPGQGLSSYPHPWLMPEFWQFPTVSMGLGPMMAIFQARFIRYMEHRG
                     LAKPSDRKVWAFLGDGEMDEPESMGALTMPVREGLDNLIFVINCNLQRLDGPVRGNGK
                     IIQELEAAFLGAGWNVIKVLWGSRWDPLLAKDHHGLLRRVMEECVDGEYQNFKAKGGA
                     YTREHFFGKYPELKAMVANMSDEEIWRLNRGGHDPRKVYAAYAAASAHKGQPTVILAK
                     TVKGFGLGKGGEGQMVAHQQKKLSEDDLRAFRDRFHIPVADEDIGRLPFRKPAEDTDE
                     YRYLRERRTSLGGSLPVRRKEAPALAIPPLETFGPLLEGTGEREISTTMAFVRMLTIL
                     LKDKNIGKNVVPIVPDEARTFGMEGLFRQIGIYSSVGQLYTPQDAETLMSYREDKKGQ
                     MIEEGINEAGSLCSWIAAGTAYSNHGISMVPFYTFYSMFGFQRVGDFIWAAGDSQARG
                     FLMGATAGRTTLAGEGLQHQDGHSQLVATTLPNCVAYDPTFAYELAVIIHDGMRRMFV
                     DQENIFYYITVMNENYRQPAMPQGVEAGIVKGGYLLQSGGRGKVRATLLGSGTILREA
                     QAAALILENDYGVPADVFSVTSYSELRREAMELERWNLMHPGETARVPYVQQMLQERE
                     GPVVAATDYVRTVPEQIRQWVPGRYVTLGTDGYGRSDSRAALRKHFEVDRHYIAVAAL
                     KALSDEGKVDQKTVTGAMQALGIDPDKPVPWKV"
     gene            6174..7772
                     /locus_tag="VFB37_13855"
     CDS             6174..7772
                     /locus_tag="VFB37_13855"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23582.1"
                     /translation="MMVKQAQAGELSARRTKSGLTIRRGSLLPSAPMPRVGRTSSRTA
                     GHLDAILVVLPEKPSADLFAQLPDHERWHELNARSRPSSGSVRSTVLANRHQTLAVLG
                     YIGAGASTFERLSLAGKMLKEASSRAPRSLGLAAPGESVAGQAALEALLAASLAQAFR
                     MPSFRAPIPDERSIERIELLTDRAGRSSAAARVDLAYALAAARGTNLTRWLTALPPNL
                     LDANGYRSVIQTLARRSGVRLRWLGEPALRRLGANAFLAVAAASQGVGAGIAHLTYRP
                     ARNRQRSPDVALVGKGILFDTGGVNLKTHRSMLDMHIDMSGSAVALATLLALAELRAP
                     IAADAWLAITENRIGPNAYRPQEVVRAANGVTIQVIHTDAEGRMVLADTLALASRTRP
                     RFMIDFATLTGACVYSLTERMSGVLTNRPDLAARLVAAGRTSGERVWNFPAEADYDSD
                     LESKVADILQCSVEGKGDHILATRFLRRFVPDDLAWAHVDLSAAMRSGGLGHINTDIT
                     GFGVRFALELILRHGILEAAGQKS"
     gene            7769..>8142
                     /locus_tag="VFB37_13860"
     CDS             7769..>8142
                     /locus_tag="VFB37_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO23583.1"
                     /translation="MTALRIRRPDDWHVHLRDGTALRAVLPFTAERFARAIVMPNLQP
                     PVTTCEQASLYRQRILEALPAGARFEPLMTLYLTDSTAPAEVDRARASGFVHGFKLYP
                     AGATTHSDAGVTDIRHLDAVLAR"
BASE COUNT         1355 a         2697 c         2597 g         1493 t
ORIGIN      
        1 cctcggtctc cgagagacct acccaggcaa tctccgggtc cgtgtaagcc accgaaggga
       61 tcacccgggc gtcgaatgcg cttttgtggc cggccgcgac ctcggcagcc accttggctt
      121 cgtgcatcgc cttgtgcgcc agcagggggg tagaggcgat gtctccgatc gcgaagatgt
      181 gcggaacgtt ggtgcgcatc tgcttgtcca cgggaatgaa gccgcgatcc gaaaccacga
      241 tccccgccgc atccgcaccg atcgccttcc cgttgggtac ccgacccact gcgaccagaa
      301 ctcgagcgaa aacctgcggc gaaggcgcgt tcctgccctc gaagctcacg cgcaagccct
      361 cctgcagcgc ctcgacgcgc gcgaccttcg tgtcgagcag aatctgctcg tagcggcctc
      421 tgatgcgccg ctcgagtggg cgcacgagat ccggatcgca acccggcatg agttgagaag
      481 tcagctcgac gacgctcacc cgggcgccga gcccctcgta aacgcacgcc atctccaaac
      541 cgatgattcc gccgccgatc acgagcaggc cgccggagaa atcggggagc tccagcgcct
      601 cggaggagtc catcacgcgc ggatcggatg gcatagccgg caggcggatg gcttccgagc
      661 ctgcggcgat gacgcaggca tcgaagtgga ttcgctcgga accgctgctg cccatcacct
      721 cgagcacgtt cgtgctgacg aatcgtccta cgccctggac gacctccacc ttgcgctgct
      781 tggccagcat gcgcagaccg ccggtgagct tcctgaccac accgttcttc cagccgcgca
      841 acttgctccg atcgatgacg ggatctgcga aagcaatccc gttagcactc atcgcctcgg
      901 catcttcgat gaccttggcc gcatgcagca gcgccttgga tgggatacag ccgacattga
      961 gacacacacc tccgagcacg ggccagcgct cgacgagggt gacctgcatg cccagatcgg
     1021 ccgctctgaa ggcggccgtg tagcctcccg gacccgaacc caacaccagc agctgggtcg
     1081 agcgattgaa ttcggcgctg cccagcttgc gtcgtcccag atcgggcatc ggcatccgca
     1141 ctgtatcgcc cgccggctcg ggccgcgctc cgcggtccgc ccgcgcctgg gcggactcca
     1201 gttgagcgcc ttctgtctgt cgcggcgcca cggaagactc gctccgcgcc gtttttcgct
     1261 gtgttgaacg ctcgctcccg ctccccggtt gcgctgtcga acgctcggcc tcggtcgctg
     1321 gcggttgagc ttcggccgct ggcggtcgtg aatcggtcgc tggcggtcgt cctgtcgagc
     1381 gctcgccgtc gggcgcctga gctttcgaac tctcgctgcc cgctgcctgc ggcggtgctg
     1441 ttgaaggctt gccctccggt gaaggcttgt cttccggtga aggcttgtct tccggtgaag
     1501 gcggctgcgc agcgggagcc tcacgttcgg cctgcttctg cttggcaatg gcggatgccg
     1561 aaagcacggg ctgtcgcacg gtgcgctcca gctcttcggc atcgaaggcc tcgctgatgg
     1621 cagcctgcgg ttcgctctgc gcactcgcct gcaggcgcgc aatgaccgag cccttggaaa
     1681 ccttgtcgcc gcgcttgagc aacacttctg cgaccgtgcc cgcagaagtc gccggcacat
     1741 ccatggaagc cttgtcggtc tcgagtgtga cgagtggtgt atcgacctcc actttgtcgc
     1801 ctgttttgat cagcacgtcg acgacgagaa tgtcggcgaa gttacccaga tcgggcacga
     1861 gcagatcgag ctgactcacg ggacggcctc gagcaggcca cgcgggtcgg cgagagtttc
     1921 agccagatag gtcgtgaatc tcaccgctgt ggctccgtct atgacgcggt ggtcataaga
     1981 cagggacagc ggcagtatca atctgggtac cagctctccg tcgcggtaaa ccgggcgcaa
     2041 cgtggcgcgt gagacgccca gaatcgccac ctcgggtgcg ttgatgatcg gtgtgaaagc
     2101 cgtccccccg aggctcccca aactcgagat ggtgaaggtg ccaccctgca tctgctgcgg
     2161 agtgagcttg cccgcgcgcg ccttctgcga cagctccgcg agatggcgtg cgatctcgta
     2221 gacgtccttg cggtcggcct cgcggatcac gggcacgacc aggccgtgac gcgtatctgc
     2281 agcaaagccg atgttgagat actttttgag gatgagattg gggccgccgg ggtccagaga
     2341 cgagttgagg gtcggaaact cctgcaaagc cttcacacag gcgcgcacga tgaaggccag
     2401 cgaggtgaga cggattccct cctgcgaggc cttctgcttc agcttcgtgc gcgcatcctc
     2461 gagggcagtg atgtccgcct cctcgaactg cgtgacgtgc ggaatgttga cccagctcgc
     2521 atgcagccgc gggcccgaga tgcgctgaat gcgcgacagc ggctgcgtct cgaccgggcc
     2581 gaattgtgcg aaatcgacgc tggggacttg cggcagggcg ccggaagctg cgcctgcggc
     2641 accggctcca gcggacagat gttgcttcac gaagcccttc acgtcctcat gcgtcacgcg
     2701 ctccttgaac ccggtcccgg ttacccgggt caggtccacc ccgagctcgc gcgcaaactt
     2761 gcgcacagag ggccccgcat gcgcccgtga aaaccccggc tcgttgatgg gcggcagcga
     2821 agtgcccgcc ggcgactcca cggaggcgga ctccgtgcgc gtggcttcgt gcttcgcggc
     2881 ccgctcctcg gaaggcgcat cccgcttctg cgtggcgacc tcgcgctcgg cctccttcgc
     2941 atccgctttc cgttccgtcc cctcggaagg cttcgcgccc gcggccgtgc ctgatgccgc
     3001 gcccgattcc tgctttgggc cagcgggagc gctcggcggt gaggcagcct ctcccggcgc
     3061 tgtcagcgcc cgtacggtcg cgatcagatc cccggtcgaa accttgccgc ccttggagac
     3121 atgcagcttc tcgatgcgcc cgcccacagt cgaaggcaca tccatggtgg ccttctcggt
     3181 ctcgagggtg acgagcggcg cttcgggctc gatcgattca ccgggcttga ccagcacgtc
     3241 gatgatgtcg acgtccttga agttgcccat gtcgggcact cgcacttcga cgaggctctc
     3301 tgccgcattc atcgggctgc cttcagactt tccagggcac cggcttgtcg ggatcgatgc
     3361 cgagcgcctg catggccccg gtaacggtct tctggtcgac cttgccctca tccgacaagg
     3421 ccttcagcgc cgccaccgcg atgtagtgtc gatccacttc gaagtgcttg cgcagcgccg
     3481 cgcgcgaatc cgaccggccg taaccatcgg taccgagtgt gacgtagcgg cccgggaccc
     3541 actggcggat ctgctcgggg acggtgcgaa cgtagtcggt tgccgcgacc actggaccct
     3601 cccgctcctg aagcatctgc tgcacatacg gcacgcgcgc ggtctcaccc ggatgcatca
     3661 ggttccagcg ctcgagctcc atggcctcgc ggcgcagctc gctgtagctg gtgacggaga
     3721 agacgtcggc cggcacgccg tagtcgtttt cgagaatcag cgcagcggcc tgggcttcgc
     3781 gcaggatggt tcccgagccc agaagcgtgg cgcgcacctt gccgcgtcct ccactctgca
     3841 gcaggtagcc tcccttcacg atgcccgctt ccacaccctg cggcatggca ggctggcgat
     3901 agttctcgtt catcaccgtg atgtagtaaa aaatgttctc ctgatcgacg aacattcgcc
     3961 gcatgccatc gtgaatgatc acggcgagct cgtaggcgaa agttgggtcg taggccacgc
     4021 agttcggcag cgtcgtcgcc acgagttgac tgtggccgtc ctgatgctgc aggccctctc
     4081 cggccagcgt ggtacggcct gcggtggcgc ccatcaggaa tccgcgcgcc tggctgtcgc
     4141 ccgccgccca gatgaaatcg cccacccgct ggaaaccgaa catcgaatag aaggtgtaga
     4201 acggcaccat gctgataccg tggttgctgt aagccgtgcc ggcggcgatc caggagcaca
     4261 gcgagcctgc ctcgttgatg ccttcctcga tcatctgacc tttcttgtcc tcacggtagg
     4321 acatgagcgt ctcggcatcc tgcggcgtgt agagctggcc cacggacgaa tagatgccga
     4381 tctgccggaa caggccctcc attccgaacg tgcgcgcctc gtcgggcacg atgggcacga
     4441 cgttcttgcc gatgttcttg tccttcaaca ggatcgtgag cattcgcacg aatgccatgg
     4501 tcgtggagat ttcccgctcg ccagtgcctt cgagcagcgg accgaaggtc tccagaggcg
     4561 ggatggcaag cgccggagcc tccttgcgtc tgaccggcaa agagccgccc aggctagtgc
     4621 gacgttcgcg cagatagcga tactcgtcgg tgtcctcagc gggcttgcga aacggcagcc
     4681 ttccgatgtc ctcgtcggca accgggatgt ggaagcgatc gcgaaaagcc cgcagatcat
     4741 cctccgataa tttcttctgc tggtgagcga ccatctgccc ctcaccgccc ttgccgagcc
     4801 cgaagccctt gacggtcttg gcgaggatca cggtcggctg gcctttgtgg gcggacgcgg
     4861 ccgcgtaggc cgcatagacc ttgcgcggat cgtgtccgcc gcgattgagc cgccagatct
     4921 cctcatcgga catgttggcg accatcgcct tcagctccgg atattttccg aagaagtgct
     4981 cgcgtgtgta ggcgcccccc ttggccttga agttctggta ttcgccgtcc acgcattcct
     5041 ccatcacgcg gcgcaacagt ccgtgatggt ccttggcgag cagcggatcc caacgcgagc
     5101 cccacagcac cttgatgacg ttccagccgg ctcccagaaa cgcagcctcc agctcctgga
     5161 tgatcttgcc gttaccgcgc accgggccat cgagtctttg caggttgcag ttgatgacga
     5221 agatcaggtt gtcgagtccc tcgcgcacgg gcatggtgag agcgcccatc gattcgggct
     5281 cgtccatctc gccatcgccc aggaaggccc acaccttgcg atcggaaggc ttggcgaggc
     5341 cgcgatgctc catgtagcgg atgaagcgcg cctggaagat cgccatcatg ggcccgagtc
     5401 ccatcgacac cgtcgggaac tgccagaact ccggcatcag ccagggatgt gggtacgagg
     5461 acagaccctg cccgggaccg cctgcttcct ggcgaaagtg acggagctgg tcctcggcca
     5521 gacgcccttc cagatacgcg cgcgcgtaga cgcccggcga ggagtgtccc tgcacgaaga
     5581 cgagatcgcc cgggtgtcgc tcggagggcg cgcgccagaa gtggttgaag ccgacctcgt
     5641 agagcgtcgc cgaggaggca taactggaca aatggccgcc aaactccgtc gatacgcggt
     5701 tcgcctgcac gaccatggcc atcgcgttcc agcgaatgta cgcctcgatg cggcgctcga
     5761 gcgcgcgatt gcccggatac tccggctcgc gcccggtggg gatgctgttg acatacgcgg
     5821 tgttcggctt gaacggcaga tacgcgccgc tgcggcgcgt gtagtcgatc agccgctcga
     5881 taatgaagtg cgcccgttcg gccccgtggg tcttcaggac cgagtcgatc gactcgagcc
     5941 attcctgcgt ttcaacggga tcgaggtcgt ccagcttggg agattcagtc atgggaagtg
     6001 ctcgagctca ttgcgtcgcc tcatgcgacc ggtgccgagg gctgcagaca gatgacagcg
     6061 cggcttgcag cgcatgatca ttgcgcaaat ggaataagat gcgccggcag caaccgacgt
     6121 ccagcgggga agtttgcgcc tgcacaagcg gcgccatgat ccgggtttca ttcatgatgg
     6181 tcaagcaagc tcaggccggg gaactcagcg cgcggcgcac gaaatcaggc ctcacgattc
     6241 ggcgcggctc gctgctgccg agcgcgccga tgcctcgggt cgggcgaacg agcagccgca
     6301 ccgcggggca tctggatgcc atcctcgtcg tcctgcccga gaagccttcg gccgacctct
     6361 ttgctcagct tccggaccat gagcgctggc acgagctcaa cgctcgctcc agaccatcct
     6421 ccggaagcgt tcgcagcacg gtcctcgcca atcggcacca gacactcgcc gtgctcggat
     6481 acattggggc gggtgcgagc actttcgaga gactgtcgct ggcgggcaag atgctcaagg
     6541 aggcctccag ccgcgcaccc cgctcgctcg ggctggcagc accaggagaa agcgttgccg
     6601 ggcaggctgc gctcgaggct ctccttgcgg catcgctcgc gcaggcgttt cgtatgccct
     6661 ccttccgagc gccgatccct gacgagcgaa gcatcgagcg gatcgagctg ttgaccgatc
     6721 gcgcaggtag gagttctgcg gcggccaggg tcgacctcgc ttatgcgctc gccgcggcac
     6781 gcggcaccaa cctgactcga tggctgacgg cgttgccacc gaatctgctg gatgcgaacg
     6841 gctaccgctc ggtcatccag actctggcgc ggcgtagcgg cgtgcgcctg cgctggctcg
     6901 gtgagccggc gctgcgccgt ctaggggcca atgccttcct ggcggtcgcc gccgccagcc
     6961 agggtgtggg ggcggggatt gcgcatctga cataccgtcc ggcgcgcaat cgccaacgct
     7021 cgcccgatgt agcgctggtg ggcaagggaa ttctgttcga cacgggcggc gtcaatctca
     7081 agacacaccg ctcgatgctc gacatgcaca tcgacatgtc cggcagcgcg gtagcactcg
     7141 ccacgctact cgctcttgcc gagctgcgtg cgccgatcgc cgccgatgcc tggctcgcga
     7201 tcaccgagaa tcggatcggg cccaatgcgt atcgtccaca ggaagtggtc cgggcggcca
     7261 acggcgtaac catccaggtc atccatactg acgcagaagg acgcatggtg ctggccgaca
     7321 cgctcgctct ggcgagccgc acgcggccgc gtttcatgat cgatttcgcg accttgacgg
     7381 gagcctgtgt gtactcgctc acggagcgaa tgagcggtgt gctcaccaac cgtccggacc
     7441 tcgccgccag gctcgtggca gccggccgca cgagcggtga gcgtgtctgg aacttccccg
     7501 ccgaagccga ctatgactcg gatctggaga gcaaggtggc agacatcctg cagtgcagtg
     7561 tggagggcaa gggagatcac atcctggcga cccgctttct gcgccgcttt gtcccggacg
     7621 atctggcctg ggcgcacgtg gacctgtcag cagcgatgcg ttcgggcggt ctgggccata
     7681 tcaacaccga catcacgggg tttggcgtgc ggtttgcgct cgagctgatt ctgaggcatg
     7741 ggatcctcga ggcggcgggg caaaagtcgt gacggcgctg cgcatccgcc gtccagatga
     7801 ctggcacgtc catctgcgtg atgggacggc tctgcgcgcg gtgctgccgt tcaccgcgga
     7861 gcgattcgca cgggcgatcg tcatgcccaa tctgcagccg cccgtcacaa cgtgcgaaca
     7921 ggcctccctt taccggcagc ggattctcga ggcgcttccg gccggcgcgc gcttcgagcc
     7981 gctcatgacg ctgtatctca ccgatagcac cgcacctgcc gaggtggacc gcgctcgagc
     8041 gagtgggttt gttcacggtt tcaaactgta ccccgcgggg gcgacgacgc attctgacgc
     8101 gggggtcacc gatatccggc acctggatgc ggtgctggcc cg
//
LOCUS       DASRHN010000346        13567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_127118, whole genome shotgun sequence.
ACCESSION   DASRHN010000346 DASRHN010000000
VERSION     DASRHN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13567
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127118"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFB37_13865"
     CDS             complement(<1..888)
                     /locus_tag="VFB37_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZO23584.1"
                     /translation="MAVDIAQLLAFAVKNNASDLHLSAGVPPMIRVDGDMKRINMPAL
                     AHKEVHSMVYDIMNDKQRKAYEEYFETDFSFEIPKLARFRVNAFNQNRGAGAVFRNIP
                     SLILSLDDLQAPKIFRDLCMLPRGLVLVTGPTGSGKSTTLAGMVNHCNDNRPDHIITI
                     EDPIEFVHESKRCLVNQREVHRDTLGFTEALRSALREDPDVVLVGEMRDLETIRLALT
                     AAETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKDMVRSMLSESLRAVISQTLLKRVGG
                     GRVAAHEIMIGTPAIRNLIR"
     gene            978..1676
                     /locus_tag="VFB37_13870"
     CDS             978..1676
                     /locus_tag="VFB37_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZO23585.1"
                     /translation="MGPQNLPEHVQSIRDRIAAAAAAAGRSVESITLLAASKDQSAES
                     VRAAAAAGVRDFGESYLQEGLDKMASLGDLPLVWHFIGRLQANKTRPVAAAFDWVHGI
                     DRLRIAERLSQQRSAHSPPLNLCLQVNLAGEPTKGGLSMSELPALAQAVAKLPRVRLR
                     GLMCLPPDETEPTRQRYWFAALRRQLESLNAAGGCLDTLSMGMSGDFEAAVLEGATIV
                     RIGTALFGPRPQAK"
     gene            1715..2545
                     /gene="proC"
                     /locus_tag="VFB37_13875"
     CDS             1715..2545
                     /gene="proC"
                     /locus_tag="VFB37_13875"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZO23586.1"
                     /translation="MSIERLAILGGGNMGRALIGGLLRRGARPEQIFVAERLDTARQS
                     LARDFGVLASEECAAAASSASLIVVAVKPQDVQSTLAPLRALLERNRPVIVSVAAGIR
                     LSALESWCGAGVPIVRSMPNRPALVGAGASGLFARPSVIPADRARAERVLQAVGEIAW
                     VCSEDELDVVTALSGSGPAYFFLIAELLTRGAAELGLDPSVARKFALATLQGAGQLAG
                     SSDGDLRRLRAEVTSKGGTTEAAVAVLESADLGGILTRAMEAATRRSRELAAQFGTGR
                     "
     gene            2562..3086
                     /locus_tag="VFB37_13880"
     CDS             2562..3086
                     /locus_tag="VFB37_13880"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZO23587.1"
                     /translation="MSALIFIIDTLLSLALFVVLARLLLQWARADFRNPLCQAVVRIT
                     NPFIVPMRRVLPPVGKIDTASVVAMLVVACIKVGVFFALHGIAPPPLAWVREVAVELV
                     RTLLMTYFYAILLYALLSLIAPGGYSPLQSVLATLCEPVLRPFRRVIPPIAGIDLSPL
                     WAVIAIQAILLLVQ"
     gene            3610..3924
                     /locus_tag="VFB37_13885"
     CDS             3610..3924
                     /locus_tag="VFB37_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZO23588.1"
                     /translation="MAKKGGAPTKSEVLTQIAKDTSLSRKQVGAVFDSLSGVIKKSLR
                     GNGLFTLPGLLKLKVVKKPATKAREGVNPFTGEKMTFKAKPASKKVRVLPLKSLKAMV
                     NS"
     gene            complement(4038..4694)
                     /locus_tag="VFB37_13890"
     CDS             complement(4038..4694)
                     /locus_tag="VFB37_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO23589.1"
                     /translation="MPHKPPHAERTVIPGPAGSLQILIETPDPQDTRHFAVVCHPHPL
                     FGGTLDNKVVYTVARACEELGAPAIRFNFRGVGSSAGAHDGVAGETEDALAVIAYGRA
                     RWPDAALWLAGFSFGGAVAIQAAVRVRPDRLIAVSPGVMRLDGPDVTAVRTRTSPWLI
                     VQGDADEVIAPERVLEWARTISPPPAIKVLAGASHFFHGRLQELRQAVLEFLQADLPA
                     "
     gene            complement(4754..6754)
                     /gene="htpG"
                     /locus_tag="VFB37_13895"
     CDS             complement(4754..6754)
                     /gene="htpG"
                     /locus_tag="VFB37_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZO23590.1"
                     /translation="MPEPENQSSGESAGKQPSTAQPGEQPATEPTSPSEAASGRQTLS
                     FQAEVRDLLKLMIHSLYSHREIFLRELISNASDANDRLRFAAIATPALLAEDPELEIR
                     VDADPAKGTLTIRDNGIGMTREEAVSNLGTIARSGTAEFFRSLSGDQQKDSALIGQFG
                     VGFYSAFIVAEKVEVLSRKAGVPAELGMRWESNAEGDFTVETVSRSERGTAVTLHLKA
                     DAREFADPFRLRSLIRRYSDHIGFPVRMRKEGEASLDYEIVNQAKALWTLPRSEISDE
                     EYRQFYQHIAHDSTDPLAWSHNKVEGKREYTSLLYLPGRAPFDLWQRDGARGLKLYVR
                     RVFIMDDAEQFLPQYLRFVKGIVDSSDLPLNVSRELLQQDPEVDAIRGGLTKRVLDML
                     ARLAKEEPQKYATFWKEFGAVLKEGLAQDFANKERILPLLRFASTHETGNEPSTSLAQ
                     YVERMKPGQDRIYYLIAESVESARSSPYIEQLRERGLEVLLLSERIDEWVMGQLDAFE
                     GKRFKDVTRGDLELGTLESEADRKEKEAVLKESKRLLKRVKDALGARVEEVRASVRLK
                     ESPACLVLGEHDLGSNMRRILNAAGQKVPESRPILELNATHPIVQYLDGMTDAAQFNE
                     LAQLLYDQAALAEGGQIANPADYLLRLNRLLVRLAGLHGAAA"
     gene            complement(6859..7038)
                     /locus_tag="VFB37_13900"
     CDS             complement(6859..7038)
                     /locus_tag="VFB37_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23591.1"
                     /translation="MEFLLLWWDELDDLTHACRHLATCALTEVAGSAPLIQGALALSS
                     WMLLHLLKPSPLNFS"
     gene            7327..8010
                     /locus_tag="VFB37_13905"
     CDS             7327..8010
                     /locus_tag="VFB37_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZO23592.1"
                     /translation="MNPKGGSGKTTIAINLASYFASREQRPVLMDFDPQGSSVRWVRK
                     RQPTQPPIQVIAAFERDTRTTRAFQLRLPDRCTHVIVDTPAAVEARQLPEVTRDAHKI
                     LVPVLPSDIDIHTCSRTIRDLLLVAKIRREEDRIGVIANRIRRNTLTYQALIRFLQSL
                     RIPIVATIRDSMNYVRAAEQGVGVHEMKSSLAQEDIEQWRPLVAWLERPGASPQPAQD
                     PPASAAVGA"
     gene            complement(8127..8273)
                     /locus_tag="VFB37_13910"
     CDS             complement(8127..8273)
                     /locus_tag="VFB37_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23593.1"
                     /translation="MQRARARHAAITKNLYTWSNYKNWADRVRDSFEDEPAPVGSPPK
                     LPKK"
     gene            complement(8555..9940)
                     /gene="ppnN"
                     /locus_tag="VFB37_13915"
     CDS             complement(8555..9940)
                     /gene="ppnN"
                     /locus_tag="VFB37_13915"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HZO23594.1"
                     /translation="MDSSIIVSDFVDARVGPRGSLENLSQDEIGNLLDSGQGGLYPLF
                     RKCALAVLNSGADTDNAKEIFDRYRDFDIEILRQAWGIKLEMRNAPAAAFVDGEMIRG
                     IKEHLFAVLRDVVFISNEIVASGRFDLTDSASITNAVFHVLRNARVLDTKLRPNLVVC
                     WGGHSIGSDEYQYTKRVGYEMGLRGLDVCTGCGPGAMKGPMKGATIGHAKQRIEHGRY
                     IGVTEPGIIAAEPPNPIVNQLVILPDIEKRLEAFVRLAHGIVVFPGGAGTAEEILYLA
                     GLLLDPVNQEQPFPVVFTGHKDSAAYFEHILSFIGATLGEEAARRFTVIIDDPAEVAR
                     HMVRGMSAVREFRRHHNDSYNFNWLLSVPAEFQRPFEVTHESMRALELDRGQPVHRLA
                     ANLRRAFSGIVTGNVKDYGIHAIERHGPYELAGDPGIMRLLDELLTAFVAQRRMKLPG
                     SIYRPVYRLVA"
     gene            complement(9967..10512)
                     /locus_tag="VFB37_13920"
     CDS             complement(9967..10512)
                     /locus_tag="VFB37_13920"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZO23595.1"
                     /translation="MPVSPGKLLIAAALLGAALAAQADSLPEPQRVMMRTTETLPVVD
                     HVLVRKSERKMFLMHGERVVRTYKIALGLNPVGTKERAGDFRTPEGAYRLIRRNPRSD
                     FFLSMQVSYPSEADLRHARRNHWDSGGSIMIHGLPNQLKHDPSYYDKTDWTDGCIAVS
                     NADMLEIWLLTPDNTPIDILP"
     gene            10710..11429
                     /locus_tag="VFB37_13925"
     CDS             10710..11429
                     /locus_tag="VFB37_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23596.1"
                     /translation="MRTSKKDQAAGAGAANIAIPTIEPAAAHARRVKLRRSIQSVIVA
                     ILPIILSGCATTTSSEPVKEKLDPDTATTVVVLNHPVELLSQSTHGSKSDPFAYIAPF
                     ETDRMGERTLYLWVSAPQDAGPVTQPQVLCNGQPLTLQPLEANSSVAGPAPSATTFAK
                     PDTEALKVELSQLKLSHAPYDAPVPWSAQWYFRLPPEGLKCLAGANGVSLETRAASGD
                     AQRFSTDRKNLATLDEFTRRY"
     gene            complement(11433..>13567)
                     /locus_tag="VFB37_13930"
     CDS             complement(11433..>13567)
                     /locus_tag="VFB37_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZO23597.1"
                     /translation="PARLLEATDEIRFQLGQADRFRAGLIRSGAWETHIAETLANLGL
                     PAELAVLPHVESSFNPGARSKVGAAGLWQFMRSTGRRYMRIDGAVDDRLDPFRATEAA
                     AQLLAYNYRLLGTWPLALTAYNHGAEGVRRAKEALGTDDIVTIVRQYKSRTFGFASRN
                     FYVSFLAALDIDHNPEKYFGPMERTGEAKFQEVVVPAYVPIASLERVLHMGPQQLRGL
                     NPALLRPVWDGQRHVPKAYHLRLPLDGEKWTTDSLAQHLSPGELYAGQPAPRRYRVRA
                     GDTVESVAEQNGVSPEALARLNRLRTSAKLRVGHTLTLPEVSAATLVAAASPPAVLAG
                     ETATGHANAVTAPSAAGALQGSAPPAVAPPERGTPASAGRSPVAPAAAPAANVSAKSA
                     AAAAVARASASEATAAGVYVVQRGESLSDIASKFGMTEAQLLQVNGIRNRDFVFEGQQ
                     LTVTPPAPALASARPTPGAGTSPAAAPIASTVVPSTNTPSHEQMAGALPLAEPSVPVV
                     AGGGVPAAVAERESVEEAAEVASTREPTENAQPVSAEQAEALSPALGPSADTQQNADP
                     IDYSVARDGTIAVAAAETLGHYADWLGTSAARLRQLNRMKFGRPVLIGHRLKLEFNRT
                     SRDDFEARRRQYHEELQAAYFAAHRIVGTEVYIVRRGDSLWTVTQRFVQLPIWLLQQY
                     NPDVDLADLHPGTQITMPRVEIVVPGPS"
BASE COUNT         2335 a         4397 c         4440 g         2395 t
ORIGIN      
        1 gcggatcaga ttccggatgg ccggcgtgcc gatcatgatc tcgtgggcgg cgacccggcc
       61 gccgccgaca cgcttgagca gtgtctggga gatcaccgcc cgcagcgact cggagagcat
      121 ggagcggacc atgtccttct ccgctgccgg aaacacgtcg acgatgcggt cgatcgtctt
      181 ggccgccgag ctggtgtgca gggtgccgaa cacgaggtgg ccggtttcgg ccgcggtcaa
      241 cgcgagtcga atcgtctcca ggtcgcgcat ctcaccgacc agaaccacgt ccgggtcctc
      301 gcgcaaggcc gagcgcagcg cctcggtgaa gccgagcgtg tcgcggtgca cctcgcgctg
      361 gttgaccagg cagcgcttgg actcgtgcac gaactcgatc gggtcctcga tcgtgatgat
      421 gtgatcgggc cggttgtcgt tgcagtggtt gaccatgccg gcaagcgtcg tggacttgcc
      481 cgagccggtc gggccggtga ccagcaccag tccacgcggc aacatgcaca gatcgcggaa
      541 gatcttcggc gcctgcagat catccagcga gagaatcagc gacggaatgt tgcggaacac
      601 ggcgccggcg ccgcggttct ggttgaaggc gttcacgcgg aagcgcgcga gcttggggat
      661 ctcgaaggag aagtcggtct cgaagtactc ctcgtacgcc ttgcgctgct tgtcgttcat
      721 gatgtcataa accatgctgt gcacttcctt gtgcgcaagc gcgggcatgt tgatgcgctt
      781 catgtcgccg tccacgcgga tcatcggcgg gacgccggcg gacagatgca gatcggacgc
      841 attgttcttg acggcgaacg ccaagagttg cgcgatatcg acagccatgg atgctcctcg
      901 gggacctgtt gcaggcggga aacctgcatc tgggcgcagc gggagtataa cggaggggtt
      961 ccgtatccaa tatgttaatg ggtccgcaga atttgccgga gcatgtgcaa agtattcgcg
     1021 accgcatcgc cgcggccgcc gcggcggccg gccggagtgt cgagtccatc acattgctgg
     1081 cagccagcaa ggatcagtcg gccgagagtg tgcgggcggc cgcagcggcg ggcgtgcgcg
     1141 atttcggcga aagctacctg caggaaggcc tggacaagat ggcatcgctg ggcgacctgc
     1201 ccctggtctg gcatttcatc ggcaggctgc aggcgaacaa aacacgcccg gtggccgccg
     1261 ctttcgactg ggtgcacggc atcgatcggc tgcggatcgc ggagcgcctc tcgcagcagc
     1321 ggtccgcaca ctctccccct ctcaatctct gcctgcaggt gaatctggcc ggtgaaccca
     1381 ccaaaggcgg gctgtcgatg agcgagctgc ccgcgctggc gcaagcggtg gccaaactcc
     1441 cgcgtgtcag gcttcgcggt ctgatgtgct taccgccgga cgagaccgag ccgacacgcc
     1501 agcggtactg gttcgccgct ctgcgacgcc agctcgagtc gctcaatgca gccggaggct
     1561 gcctcgacac gctctcgatg ggcatgagcg gcgacttcga agccgccgtg ctggagggcg
     1621 ccaccatcgt gagaatcggc accgcgctgt tcggccccag gccgcaggcc aagtgagcca
     1681 gtggcgggct tcacagcagg tacaatcgga gtgcatgagc atcgagcggc tggcgatcct
     1741 gggcggcggt aacatgggac gcgcgctcat tggggggctg ttgcgccggg gcgcgcggcc
     1801 cgagcagatc ttcgtggccg agcgcctgga tacggcgcgc cagtcgctgg cgcgcgactt
     1861 cggcgttctg gcgagcgaag agtgtgcggc ggccgcatcc tcggcctcgc tgatcgtcgt
     1921 tgcggtcaaa ccgcaggatg tgcagtcgac tctggcaccg ctgcgagcgc tgctcgagcg
     1981 gaaccggccg gtgatcgtgt ccgtggcggc aggcatccgg ctcagcgcgc tggagagttg
     2041 gtgcggtgcg ggggtgccca tcgtccgctc gatgccgaac cggcctgccc tcgtcggcgc
     2101 aggagcctcg ggcttgttcg cacgcccatc ggtcatccca gcggatcgcg cacgtgccga
     2161 gcgtgtgctg caggcggtag gcgagatcgc ctgggtctgc agcgaagatg agctcgatgt
     2221 ggtgacggcc ctgtcgggca gcgggcccgc ctatttcttc ctgatcgccg aattgctgac
     2281 gcgcggcgcg gccgagctgg ggctggaccc ttcggttgcg cggaagtttg cgctggcgac
     2341 gttgcagggc gccgggcaac tcgctggcag cagcgatgga gacctgcgcc ggctgagagc
     2401 agaagtcacc tccaaggggg gcacgaccga agcagcagtc gctgtactgg agtccgccga
     2461 tctcggcggg atcctgactc gggccatgga ggcagcgacg cgacgcagcc gcgagctcgc
     2521 agcgcaattc ggtaccggcc ggtgacgggg aaggccaatc catgagcgcg ctgatcttca
     2581 tcatcgacac gctgctctcg ctcgccctgt tcgtcgttct ggcgcgattg ctgctgcagt
     2641 gggcccgggc ggacttccgc aatcctttgt gccaggccgt ggttcgcatc accaacccgt
     2701 tcatagtgcc gatgcggcgt gttctgccgc ccgtcggcaa gatcgacacg gcatccgtcg
     2761 tggcgatgct cgtcgtcgcc tgcatcaagg tcggggtgtt cttcgccctg cacggcattg
     2821 cgccgccacc actggcctgg gtgcgcgagg tcgccgtgga gctcgtgaga accctgctga
     2881 tgacctattt ctacgccatc ctgctctatg cgctgctgag tctgatcgcc ccgggcggct
     2941 actcgccgct gcaatccgtg ctcgcgacgc tgtgtgagcc tgttctgcgc ccgttcagac
     3001 gggtgattcc gccgatcgcg ggtatcgatc tctcgccgtt gtgggcggtc atcgcgatcc
     3061 aggccattct gcttctggtc cagtgagccg cgcggcgcct cattgaggcc gaccagaacg
     3121 tgcagtgcgc ggcgtgcgct tgcggattgt gcgtacccgc tgccgcgctc ccgcatcgac
     3181 gacgcacgtg cgatctcggc ggagcaacga gactgcagcg aggctgctct gcaccgcgat
     3241 ccgccccgca tgtgattccc gcgccgcgcg tggctcgcgc gccagcgcta cacggtgcga
     3301 gcaaatgcct gtattccgcg gcaaaatcag cgacacacgc tgttgcgaaa cacgctctta
     3361 gagagcactc cgcacgcttc agtcgaagtc gacgcgcggc gctgcagcgt cgatcgggcg
     3421 cgcacgcagg agcgcggcgg ccggcccggg ttttgccccg agcgcgcaaa ccctgaaaaa
     3481 aaaggcattt gcgcaccagc gaagagaaaa aaatgcttgc caacgcgtgt gcatgtgcta
     3541 tttttcgcgc cgattctcat cgcgcggtgc accgcgctca cagtcactac attcgaggag
     3601 tagcgcaaaa tggcgaaaaa aggtggcgca ccgaccaagt cggaggtcct gacgcagatt
     3661 gccaaggaca ccagtctctc ccgcaagcag gtcggagccg tcttcgattc gctgagcgga
     3721 gtgatcaaga agagcctgcg gggcaacggc ctgttcaccc tgcccggcct gttgaaactg
     3781 aaggtcgtga agaagccggc cacgaaggca cgtgagggcg tcaatccctt caccggcgaa
     3841 aagatgacat tcaaggccaa gcccgcgagc aagaaggtgc gcgtgctccc cttgaagagt
     3901 ctgaaagcaa tggtcaattc ctagaattct ttttgcggaa ttctttgcgg ggactttttt
     3961 cccgccgcaa ggcagtgcaa tcgacccggt gagtcgctcc tcaccgggtt gattttttta
     4021 ggtggtcagg ttcgccctca ggcgggcaga tccgcctgca ggaactcgag caccgcctgg
     4081 cgcagctcct gcagccggcc atgaaagaaa tgactcgccc ccgcgagtac cttgatggct
     4141 ggcggcggag agatcgtccg tgcccattca agcacccgct ccggcgcaat cacctcatcc
     4201 gcatcgccct gtacgatgag ccacggtgaa gtgcgtgtgc gcaccgcagt gacatccggg
     4261 ccatccaggc gcatcacccc cggcgagacg gcgatcagac gatctggcct gactcggacc
     4321 gccgcctgga tcgccacggc accgccgaag gagaatccgg ccagccacaa ggccgcatcg
     4381 ggccagcgag cccttccgta ggcgatcacc gcgagagcat cctcggtctc accagcgacc
     4441 ccatcgtggg cgccagcgct cgagccgaca ccacggaaat tgaagcggat cgccggcgct
     4501 ccgagctcct cgcatgcgcg cgccaccgtg tagaccactt tgttgtcgag cgttccgcca
     4561 aacagcggat gcggatgaca caccacggca aagtggcgcg tatcctgcgg atccggcgtc
     4621 tcgatcagga tctgcagact cccggccgga ccgggaatca cggtgcgctc cgcgtgcggt
     4681 ggtttgtgcg gcacagcaga gagacgcgct agtgctgggt cagcattcgc aacccgtgcg
     4741 ggcggccagc atgctacgcg gcggcgccat gcagccccgc gagccgcacg agaagccggt
     4801 tcagacgcaa caggtaatcg gccggattcg caatctggcc cccctcggcg agtgccgcct
     4861 ggtcgtacag cagctgcgcc agctcgttga attgtgcggc atcggtcatg ccatccagat
     4921 actgcacgat cggatgagtc gcgttcagct cgaggatggg tcgcgactcg ggcactttct
     4981 gtcccgccgc attcaggatg cgccgcatgt tcgagcccag atcgtgctct cccagcacca
     5041 ggcacgccgg agattccttc agacgcacgc tcgcgcggac ctcttccacc cgtgcgccga
     5101 gcgcatcctt gacccgcttc agcagccgtt tgctctcctt gaggacggcc tccttttcct
     5161 tgcgatccgc ctcgctctcc aaagtcccaa gctccaggtc ccctcgtgtc acgtccttga
     5221 agcgcttgcc ttcgaatgca tcgagttgac ccatcaccca ctcatcgatc cgctccgaca
     5281 gcagcaggac ctcgagcccg cgttcccgca gctgctcgat gtaggggctc gagcgagcgc
     5341 tctccacgct ttcggcgatg agatagtaga tacgatcctg accgggcttc atccgttcca
     5401 catactgcgc caggctcgtg ctgggctcgt tgcccgtctc gtgggtgctc gcgaagcgca
     5461 gcagcggcag gatccgctcc ttgttggcga agtcctgcgc caggccttcc ttcaggacgg
     5521 caccgaactc cttccagaag gtcgcatact tctgcggctc ttccttggcc agcctcgcca
     5581 gcatgtccag cacccgcttg gtgagtccgc cccggatcgc atccacctcc gggtcctgct
     5641 gcagcagctc gcgcgagacg ttcagaggca ggtcgctcga atccacgatg cccttcacga
     5701 aacgcaggta ctgcggcagg aactgctcgg cgtcgtccat gatgaacacg cggcggacgt
     5761 aaagcttcaa cccgcgagct ccgtcgcgtt gccacaggtc gaacggggcg cgcccaggca
     5821 ggtacagcag gctcgtgtac tcgcgcttgc cctcgacctt gttatggctc caggccagcg
     5881 ggtcggtcga gtcgtgagcg atgtgctgat agaactggcg gtactcctcg tcggaaatct
     5941 cagaccgcgg cagtgtccac agagccttgg cctgattcac gatctcgtag tcgagcgagg
     6001 cctcgccttc tttgcgcatc cgcacgggaa agccgatgtg atccgaatag cgacggatca
     6061 gcgagcgcag gcggaacgga tcggcaaact cgcgggcatc tgccttcagg tgaagcgtga
     6121 ccgctgtgcc gcgctcggac cggctcacgg tctccaccgt gaagtcacct tccgcgttcg
     6181 attcccaccg cattccgagc tcggccggca ctccggcctt gcgggacagc acttccacct
     6241 tctcggcgac gatgaatgcg gaatagaagc cgacgccgaa ctgcccgatc agcgccgagt
     6301 ccttctgctg gtcccccgag agcgagcgga agaactccgc ggttccggag cgggcgatgg
     6361 tgcctagatt ggacaccgcc tcctcgcgtg tcatgccgat tccattgtcg cggatggtga
     6421 gggtgccttt ggcagggtcg gcatccacgc ggatttcgag ctcggggtcc tccgccagaa
     6481 gcgccggtgt cgcgatcgcc gcgaagcgca gccgatcgtt cgcatcggag gcattcgaga
     6541 tcagctcgcg caggaagatt tccctgtggc tgtacaggga atggatcatg agcttcagca
     6601 ggtcgcggac ttcagcctgg aagctcagag tctgcctgcc gctggccgcc tcagacgggc
     6661 tcgtgggctc ggtcgctggc tgctcaccgg gttgggcggt gctgggttgc ttcccagccg
     6721 actcgccgct gctctgattt tcgggttctg gcatcgtcct tcccgccggc ggcggctggc
     6781 gccgccgctc tctcgccaat acgtgcacgg caggcgccca cgggcgcgcg ccttcaggaa
     6841 gggcgatctt gggaagatct aggaaaaatt caaggggctg ggcttgagca ggtgcagcaa
     6901 catccagctg ctcagcgcaa gggcgccctg tatcaggggc gcggaacctg cgacttcggt
     6961 aagtgcgcag gtcgcgaggt ggcgacatgc gtgggtgaga tcgtcgagct catcccacca
     7021 gaggaggaga aattccatgc gctgcagact acggatccaa tgtggcagtt ggtgtgcgcg
     7081 aactcacagt caccgcaggc ccggctgagc gaaagtccga acgcgcgcag cgatctcgaa
     7141 tcgtgctgcg gcttgcacga cataacctgg gtgcgtacgc ttgaccatga cgatcattta
     7201 ccgcagaagg cgcgcgtgcg gcgccttgtg cgcgcggaca acagggcacg gtgcacgcgc
     7261 cgcgcccgtt gaccgctcgt aagggcgaaa ctgacaaacc acgccactat ccagcgcatc
     7321 gtcgtcctga accccaaggg aggctcgggc aagacgacta tcgcgatcaa cctggcgagc
     7381 tacttcgcct cccgggagca aagaccggtc ctgatggatt tcgatccgca gggctcctcg
     7441 gtgcgctggg tgcgcaagcg tcagccgacg cagcccccga tccaggtcat cgccgctttc
     7501 gagcgcgaca cccgcacgac gcgggccttc cagctccgcc tgcccgatcg ctgcacgcac
     7561 gtcatcgtgg acacgcccgc cgccgtggag gcgcgccagc tccccgaggt cacccgcgat
     7621 gcgcacaaga tcctggttcc cgtgctgccc tcggacatcg atattcacac ctgctcacgg
     7681 accatccgcg acctgctgct ggtcgccaag atccgccgcg aggaggatcg catcggtgtc
     7741 atcgccaatc gcatccggcg caatacgctc acctatcagg ccctgatccg cttcctgcag
     7801 agcttgagga ttccgatcgt cgccacgatc cgtgactcga tgaattacgt gcgggcggcc
     7861 gaacaggggg tcggcgtgca cgagatgaaa agctctcttg cgcaggagga catcgagcag
     7921 tggcggcccc tcgtcgcctg gctggagagg ccgggggcca gcccacaacc ggcgcaggat
     7981 ccgcccgcat cggcggcggt gggcgcctga cggaggccgc tgcggggcac gaagaggttt
     8041 ggagtcagcg cgcgccgcaa gggcgtgcga tcaccgacgc gaatcgccct caggcttgcc
     8101 gcgctcgccg cgagggcgag cgcgagctat ttcttgggaa gcttgggcgg cgatccgacg
     8161 ggggccggct catcctcgaa ggaatcgcgc acgcggtccg cccagttctt gtagttggac
     8221 cacgtgtaga gattcttcgt gatggcggca tgacgcgcgc gggcgcgctg catgcgatcc
     8281 agccagtttg cgtacggatc ggcggggtcc ggggaactcg gagtcttcgc agcgggtatg
     8341 aggtccggcc gcgcgccagg cgtgtctcct ctccccctgt ctgatgagcc cattgtcgct
     8401 cctgatgctt tcacgcagag tacggtctac tcgcgaagcg gcgcaggaac tggttcacag
     8461 gggtttcgcg tgcagaagag gctgggacgc agctctcgcg tccgcgcagt ccgctgcgaa
     8521 ccacgcgagg cgttatgtca cgcgaagtgt cggttcaggc aacgagacgg tagacaggac
     8581 gatagatgct gcccggcagt ttcatccgcc gctgcgcgac gaatgccgtg agcagctcat
     8641 cgagcaaccg catgatcccc ggatcccccg ccagctcgta aggtccatgg cgctcgatgg
     8701 cgtgaatgcc atagtccttc acgttgccgg tcacgatgcc tgagaaggcg cgacgcagat
     8761 tggccgccag gcggtgcacg ggctggccgc gatcgagctc cagcgcacgc atcgactcgt
     8821 gggtcacctc gaagggccgc tggaattcag caggcaccga cagcagccag ttgaagttgt
     8881 aggaatcgtt gtgatggcgg cgaaactcgc ggaccgcgct catgccgcgc accatgtgcc
     8941 gtgcgacctc cgccgggtca tcgatgatca cggtgaagcg gcgcgccgcc tcctcgccca
     9001 gcgttgcgcc gatgaagctc aggatgtgct cgaagtaggc ggcgctgtcc ttgtggccgg
     9061 tgaacaccac gggaaagggc tgttcctgat tcaccggatc gagcagcagc ccggccaggt
     9121 acaggatctc ctcggccgtg cccgccccgc cgggaaacac cacgatcccg tgcgccaggc
     9181 gtacgaaggc ctcgaggcgc ttctcgatgt ccggcaggat gacaagctgg ttcacgatgg
     9241 ggttcggcgg ctcggccgcg atgatccccg gctcggtgac gccgatgtag cggccatgct
     9301 cgattcgctg cttcgcatgc ccgatggtcg cgcccttcat cggccctttc atggcccctg
     9361 gaccgcagcc cgtgcacacg tccagcccgc gcaggcccat ctcgtagcca actctcttcg
     9421 tgtactggta ctcgtccgag ccgatcgagt gtccgcccca gcacaccacg aggttgggcc
     9481 gcagcttggt gtcgagcaca cgcgcgttgc gcagcacgtg aaagacagcg ttggtgatgg
     9541 aggccgaatc cgtcaggtcg aaccggccac tcgccacgat ctcgttcgag atgaacacca
     9601 catcgcgcag cacggcgaag aggtgctcct tgataccccg gatcatctcc ccatcgacga
     9661 aggcggccgc cggggcattg cgcatctcga gcttgatacc ccaggcctgg cgcaggatct
     9721 cgatgtcgaa gtcgcggtag cggtcgaaga tttccttggc gttgtcggtg tctgcaccgc
     9781 tgttgagcac ggcgagggcg cacttgcgaa acaggggata caggccaccc tgccctgaat
     9841 cgagcaggtt gccaatctca tcctgcgaca ggttctcgag gctgccgcgc ggaccgactc
     9901 gagcgtcgac gaaatcggaa acgatgatgc tgctgtccac tcgcgcgcgg ctgctctgcc
     9961 gtagggctac ggcagaatat cgatgggcgt gttgtcgggc gtcagcagcc agatttcaag
    10021 catgtcggca ttcgacacgg cgatacagcc gtccgtccag tcggtcttgt cgtagtagct
    10081 gggatcgtgc ttgagctggt tgggcaggcc gtgaatcatg atcgagccgc cactgtccca
    10141 gtggttgcgg cgggcatgcc tgagatccgc ctcgctcgga taagacacct gcatggagag
    10201 gaagaagtcg ctgcgcggat tgcggcgaat cagacgataa gcgccctcag gcgttctgaa
    10261 gtccccggcg cgctccttgg tgcccaccgg attgaggccc agcgcgatct tgtaggtgcg
    10321 cacgacgcgc tcgccgtgca tcaggaacat cttgcgctcg gacttgcgga ccagcacgtg
    10381 atccacgacc ggcagagtct cggtcgtgcg catcatgacg cgctggggct ccggcaggct
    10441 gtcggcctgc gcggcgagtg ccgcaccgag cagcgcggcg gcgatcagga gcttcccggg
    10501 ggagacaggc attattcggt caaacaagga cttgcaaagt taaacgcatt ataggcgctc
    10561 atccggcgtg ccgcgatgta cattcgtcac agcccgccca tgtgggaacc acgaggcgcg
    10621 ccgtgagttt gactgcagat cacactttgc gttctatact gaggcgtgcg gcgaccgccg
    10681 gttgccgccg taatcagggg ccgaacataa tgagaacatc aaaaaaggat caggccgcgg
    10741 gggccggtgc cgcaaacatc gcgatcccaa caatcgagcc cgccgctgcc cacgctcggc
    10801 gggtcaagct tcgcaggtcg attcagagcg tcatcgtcgc gatcctgccg atcatcctga
    10861 gcggctgcgc caccacgaca tccagcgagc ccgtgaagga gaaactcgat ccggacacgg
    10921 ccaccacggt cgtggtgctg aatcatccgg tggagctgct ctcccagagc actcacgggt
    10981 ccaaatccga tccgtttgcc tacatcgcac cgttcgagac cgaccggatg ggcgagcgaa
    11041 cgctgtacct gtgggtctcg gcgcctcagg atgcgggccc agtgacgcag ccgcaggtgc
    11101 tgtgtaacgg tcagccgctc accctgcagc cgctcgaggc aaactcctcc gtggccggtc
    11161 cggccccaag cgcgaccacc ttcgcgaagc ctgacaccga ggctctcaaa gtggagttgt
    11221 cgcaactcaa gctctcgcac gcgccctacg acgctccggt tccctggagt gcgcagtggt
    11281 atttccggct gccccccgaa ggcctgaagt gcctcgccgg cgcgaacggg gtttcgctcg
    11341 agacccgtgc cgcaagcggc gacgcgcagc gtttcagcac ggatcgcaag aacctcgcca
    11401 cgctggacga gttcacccgt cggtactgag gttcagctcg gtcccggaac gacgatctcc
    11461 acgcgaggca tcgtgatctg cgtgccggga tgcagatcgg cgagatccac atcggggttg
    11521 tattgttgca gtagccagat cggcagctgc acgaaccgct gggtcactgt ccacagtgag
    11581 tcgccgcgcc ggacgatgta aacctccgtg cccacgatgc ggtgagcggc gaaataggcc
    11641 gcctgcagct cctcgtggta ctggcgccgt cgcgcctcga agtcatcccg cgaggtccgg
    11701 ttgaattcca gcttgagccg atgcccgatc agcacaggcc ggccaaactt catccggttg
    11761 agctggcgca gccgcgccgc cgacgttccg agccaatcgg cgtaatggcc gagcgtctct
    11821 gctgcggcta ccgcgatcgt gccgtccctg gctaccgaat aatcgatcgg gtccgcattc
    11881 tgctgagtgt ccgccgaagg tccgagcgcg ggactcagcg cctcggcttg ttcggccgag
    11941 acaggctgcg cgttctctgt aggttctctc gtgctcgcga cctcagcggc ttcctccacg
    12001 ctttcacgct cggccaccgc agccggtacg cctccgccgg caaccaccgg gaccgagggc
    12061 tccgccagtg gcagggcgcc agccatctgc tcgtggctcg gcgtgttggt gctcgggacc
    12121 acggtgctcg cgatgggcgc ggcggctggg ctcgtacccg cacccggcgt cggcctcgcc
    12181 gatgcgagcg caggcgcagg cggtgtgacg gtgagctgct ggccctcgaa gacaaaatca
    12241 cgattacgga taccgttgac ctgcagcaac tgcgcctccg tcatgccgaa cttggaggcg
    12301 atgtcggaca aggactcgcc gcgctgcacg acgtacacac cggccgcggt agcctcactg
    12361 gccgaggcgc gtgcgacggc cgctgcagcg gctgatttcg cactcacgtt cgcagcgggg
    12421 gcagccgcag gcgccaccgg tgagcggccc gcggatgcag gggtgccccg ctctggcggg
    12481 gcgacggctg gaggcgcact gccctgtagc gctcctgcgg cgctcggggc cgtgacagcg
    12541 ttggcgtgac ccgtggcggt ctcacctgcg aggaccgctg gcggcgaggc tgccgcaaca
    12601 agtgtcgcag cgctgacttc cggcaacgtg agcgtatgac ctacgcgcaa cttcgcgctg
    12661 gtccgcaggc gattcagccg ggccagggct tcgggtgata cgccattctg ctcggcgacg
    12721 ctctcgacgg tatctccagc gcgaacgcga taacgacgcg gcgccggttg tccggcgtac
    12781 agctcgcccg gcgacagatg ttgcgcaagt gagtcggtcg tccatttctc accgtcgagc
    12841 ggcagacgca gatgataggc cttgggtacg tgacgctggc cgtcccatac cggacgcagc
    12901 agagctgggt tgaggccacg aagctgttgc ggccccatgt gcaagacacg ctcgagcgat
    12961 gcgatgggca cgtaggccgg cacgacgacc tcctggaatt tggcttcgcc cgtgcgctcc
    13021 atgggcccaa agtacttctc ggggttgtgg tctatgtcga gtgcggcgag aaaggagacg
    13081 tagaagttgc gcgaagcaaa gccgaacgtg cggctcttgt attggcggac gatggtaacg
    13141 atgtcatcgg ttccgagggc ctccttggcg cgacgcacgc cttcagcgcc atggttgtac
    13201 gcagtcagcg ccaggggcca cgtgcccagc agacggtagt tgtatgcgag aagctgagct
    13261 gccgcctcgg ttgcgcggaa aggatccagg cgatcatcca ccgcgccgtc aatgcgcatg
    13321 tatcgcctgc ctgtcgagcg catgaactgc cacagtccgg cagccccgac cttggagcgc
    13381 gcccccggat tgaaggatga ctcgacgtgc gggagcaccg cgagctcggc gggcaggccc
    13441 aggttggcca acgtctccgc gatatgcgtt tcccatgcgc cggagcgaat cagcccggcc
    13501 cggaatcggt cggcctggcc gagctggaag cggatttcgt ccgtcgcctc cagaagtcgc
    13561 gcggggg
//
LOCUS       DASRHN010000347         6008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_290490, whole genome shotgun sequence.
ACCESSION   DASRHN010000347 DASRHN010000000
VERSION     DASRHN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290490"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFB37_13935"
     CDS             complement(<1..629)
                     /locus_tag="VFB37_13935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO23598.1"
                     /translation="MIAATWPLLLGMGVLMLGAGLQSTLLGLRATAEGFPPQVTGIIM
                     SGYYVGYLAGTAAAPRLLRQVGHIRVFAALAAVASATILVQAAFVEPVVWVAMRLTSG
                     VCFAGIYVVAESWLNDRATPAHRGRLLSVYMLVLYVGLGAAQFLILPANPNTTTPFML
                     VSVLISLSMVPIVVSAQQAPEPLVPRKVRYRDLYRNSPLGVVAVSVSGM"
     gene            complement(626..1426)
                     /locus_tag="VFB37_13940"
     CDS             complement(626..1426)
                     /locus_tag="VFB37_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23599.1"
                     /translation="MGSVKAACAAVAAAALISMASGCASTDEPGVQHGHLVQPPTTEG
                     PFCGAQHPFGEIKGGEVERAVTLKSAEPMAIAADDHPSDAGKLLVLNHPLDPLPPEVA
                     RIRRGIVKRFTGKFIRDGQMSWSGLDLERLVAVSVERRVFDLRAHQSREVPDPVFTQT
                     AQLSFARKWTDGQRTEVEVVEVFPITLVEAQFFVCAANPLWADKKPLPEVEDVSGDLS
                     DGTTDVFLLDRRQQQDVTYRYTKTVYMSPNLAAVLDGIWQHAPKGPTW"
     gene            1502..3022
                     /locus_tag="VFB37_13945"
     CDS             1502..3022
                     /locus_tag="VFB37_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZO23600.1"
                     /translation="MLTHLDPASALAAGLLIFALLMVVIFEATNGFHDAANAVATVIY
                     TRSLTPTQAVVWSGFLNFLGVLLGGIAVAYALVELLPAEVLSPPDGGPAVAMLIALFV
                     AALVWNIGTWWFGLPNSSSHCLIGALIGVALGNAVARSRSLTDGVHWHQLWNVLKALA
                     LSPILGFVLAGALYLIIRKTIRDKHLYEPANDRPPVWWMRAILILTCAGVSFAHGTND
                     GQKSIGLIMLTIIGLFPATYALNPQAGKQIAELPKVMREAEPLVTRYGDDEKHAALAA
                     ARSIEQGAVAAQSAQANSDGGSPPAQSNSGASASGNGNAAQVRLAALELDQPHGLELG
                     PNAPAAKQRASVRDDIYRVISELKHVEEVRDASKEDKQHATQLAKQLGHSVEYAPDWV
                     RLLSALCLGIGTMIGYRRIVTTLGERLGNVHLTPAQGAAAEVVSTVLIGTAGFTGLPV
                     STTHIVTSGIGGTMVTSGAGLRYRMLRRIISAWLLTLPVTIITAGVLYYLLANPRL"
     gene            3080..3442
                     /locus_tag="VFB37_13950"
     CDS             3080..3442
                     /locus_tag="VFB37_13950"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO23601.1"
                     /translation="MLNHVINLDRIFHALADPTRRAMIERLGRAPASVSELANPMSVS
                     LAAIVQHVQILEASGLVRTEKVGRVRTCRLDAGTLMQAERWLEARRGEWNARLDRLGE
                     YLEETTHPPTPTPKKGQK"
     gene            3489..3953
                     /locus_tag="VFB37_13955"
     CDS             3489..3953
                     /locus_tag="VFB37_13955"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO23602.1"
                     /translation="MTVATHHGQFTLERTYRATPERVYAAWSEVGAKAHWFIGPEGWR
                     ELRREMDFRVGGHELLEGRYGSERVTRYTARFHALEAARRIVFVYDMHLSDVHHSTSL
                     ATVELAATANGTRLRFTEQVAFLDGTTSADSRAHGTGAHLDRLALYLARDTF"
     gene            4071..4886
                     /locus_tag="VFB37_13960"
     CDS             4071..4886
                     /locus_tag="VFB37_13960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO23603.1"
                     /translation="MQTHYVRAHDGTQLYWTEWGSGPPILFSNSAGMPTEMWDYQMGA
                     LADHGFRCIAFDRRGHGRSDVPPRGYDFDTFADDMAALIQALDLKQLTLIAHSMGGGE
                     VVRYLTRHGNQRVSRIVLIGSTTPFQLQAADNPNGIPAANFEALRASWRKDYPRWIAE
                     NTAPFFVPETSPAMARWLSGLLMRFPARIAVALNEAIASADFRRELPRISVPALVIHG
                     DRDVSAPLALTGKPTAELIPGCRFKVYEGAPHGLLYTHMDRLHADILEFIAET"
     gene            5003..5356
                     /locus_tag="VFB37_13965"
     CDS             5003..5356
                     /locus_tag="VFB37_13965"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZO23604.1"
                     /translation="MQYMLLAYADVNQFLKMTPEEQKQTYGAYQSFLEAAKKAGAYQA
                     SGRLQPASTATSVRVSNGKTQVLNGPYMETKEQLGGFFLLDVPDLDAAISWAARCPAS
                     LHGTVEIRPMWISQP"
     gene            5379..>6008
                     /locus_tag="VFB37_13970"
     CDS             5379..>6008
                     /locus_tag="VFB37_13970"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZO23605.1"
                     /translation="MTSGRGGEQARTMADAVARRSYGKLVAFLAARTRDVASAEDALS
                     EAFASALADWPVNGCPSNPEGWLLTAARRKLIDFARRRRTGETASEPLRLLAEELETA
                     ARDNEEMEIPDQRLALMFACAHPAIEASVRAPLILQVLLGLDAAMIASAFLTSPAAMS
                     QRLVRAKDKIRQAAIPFRIPDREELPGRLGAVLDAIYAAFSEGWTDSGGT"
BASE COUNT         1091 a         1964 c         1905 g         1048 t
ORIGIN      
        1 atcatcccgg aaaccgacac cgcgaccacc ccgagcggcg agttgcgata gaggtcccgg
       61 tagcgcacct tgcgcggcac caggggctcg ggcgcctgct gcgccgacac cacgatcggg
      121 accatcgaga gcgagatcaa caccgagacc agcatgaacg gcgtggtcgt gttgggattc
      181 gccgggagga tcaggaattg ggccgcgccc aacccaacat aaagaacgag catgtacacc
      241 gagagcagcc ggccgcggtg agccggggtc gcacggtcgt tcagccagct ctcggcgacc
      301 acgtagatgc cggcgaaaca cacacccgag gtcagccgca tcgccaccca gacgaccggc
      361 tcgacaaaag ccgcctgcac caggatggtg gccgaagcga ccgccgcgag cgcggcgaat
      421 acgcggatgt gtccgacctg gcgcagcaag cgcggggcgg ccgcggtgcc cgcaaggtag
      481 ccgacatagt aaccggacat gatgatgccc gtgacctgcg gaggaaatcc ttcggccgtg
      541 gcacgcaggc cgagcagcgt gctctgcagt cccgctccca acatgagcac gcccatgccc
      601 aggagcaagg gccaggttgc cgcgatcacc aggtcggccc tttgggcgcg tgctgccaga
      661 tgccgtcgag aactgccgcg agattcgggc tcatgtacac cgtcttggtg tacctgtagg
      721 tcacatcctg ctgctggcgg cggtcgagca ggaatacatc cgtggttccg tctgacaggt
      781 cgccgctcac atcctccacc tccggcaacg gcttcttatc cgcccagagg ggattggcgg
      841 cgcacacgaa gaactgcgcc tccaccagcg tgatcgggaa aacctcgacc acctcgacct
      901 cggtgcgctg cccgtcggtc cacttgcgcg caaagctgag ctgcgcggtc tgggtgaaca
      961 ccgggtcggg cacctcccgg gactggtgcg cccgaagatc aaacacccgg cgctccaccg
     1021 acacggccac caggcgctcc agatccaagc ccgaccagct catctggccg tccctgatga
     1081 acttacccgt gaagcgcttg acgatacccc ggcggatacg cgcgacctcc ggtggcagcg
     1141 gatccagggg atgattgagg acgagcagct tccccgcgtc cgatgggtga tcgtcggcag
     1201 cgatggccat cggctcggcc gacttcagcg taaccgctcg ctccacctcg ccgcccttga
     1261 tctccccaaa cggatgctga gcgccgcaga agggaccctc ggtcgtcggc ggctgcacga
     1321 ggtggccatg ctgcacgccc ggctcgtcgg tggaggcaca gccgctcgcc atcgaaatca
     1381 gcgcggccgc cgccacagcg gcacacgcgg ctttcactga acccatgatg aaatatttgt
     1441 tacggatgga acgggtacgt acgagtggtt ttgatgagta tagcttcatg gaggggccgc
     1501 catgctgacc cacctcgacc ccgcctcggc actggcggca ggactgctga tttttgccct
     1561 gctcatggtt gtgatcttcg aggccacgaa tggtttccac gacgcggcca atgccgtggc
     1621 tacggtgatc tacacgcgct cgctcacccc gacgcaagcc gtggtgtggt cagggttcct
     1681 gaacttcctg ggcgtactgc tgggcggcat cgcggtcgcc tacgcactcg tggagctgct
     1741 gccggcagag gtgctctcgc ctcccgacgg aggacccgcg gtcgcaatgt tgatcgcgct
     1801 gttcgtggct gctctcgtct ggaatatcgg cacgtggtgg tttgggctgc ccaattcgag
     1861 ctcccactgt ctgattggtg cattgatcgg cgtggcgctg gggaatgcgg tggcgcgctc
     1921 acgtagcctc accgacggcg tgcattggca ccagctctgg aacgttctca aggcgctggc
     1981 cctctccccg atcctagggt tcgtgctggc gggtgcgctc tacctgatca tccggaaaac
     2041 gatccgagac aaacacctgt acgagcccgc gaacgatcgg ccgccggtgt ggtggatgcg
     2101 cgctattctc atcctgacgt gcgccggtgt cagcttcgct catggcacga acgatgggca
     2161 gaagagcatc ggcctcatca tgctcaccat cattggacta ttccccgcaa cgtacgcgct
     2221 gaatccgcag gctgggaagc agatcgccga gctgccgaaa gtcatgcgag aagcggagcc
     2281 gctggtgacc cgatatggcg atgacgagaa gcacgcggcg ctcgcggccg cgcggtcgat
     2341 cgagcaagga gccgttgcgg ctcaatctgc ccaggcgaac agcgatggag gaagccctcc
     2401 tgcacaaagc aattcgggcg cgagcgcgtc gggtaacggc aacgcggcgc aggtcaggct
     2461 cgccgcgctc gagctggacc agccccatgg actcgagctc ggccccaacg caccggccgc
     2521 gaagcagcgc gcgagtgttc gcgacgacat ataccgggtg atctccgagc tgaagcacgt
     2581 ggaggaggtg cgagacgcct cgaaggaaga caaacagcac gcgacccagc tcgccaagca
     2641 gctcgggcac tcggttgaat acgcaccaga ctgggttcga ctgctcagcg ccctctgtct
     2701 cggcatcggc accatgatcg gctatcgccg tatcgtaacc accctgggcg agcggctcgg
     2761 taatgttcac ctgacgcccg cgcaaggtgc cgccgcggag gtggtctcga cagtgctcat
     2821 cggcactgcg ggtttcacag gcctccccgt aagcacaacc cacatcgtta catcggggat
     2881 tggcggcacg atggtgacct cgggtgcggg actgcggtat cgcatgcttc ggcgcatcat
     2941 ttccgcatgg ctgctgactc tgccggtcac catcatcacg gccggcgtgc tctactattt
     3001 gttggccaat ccgcgtctgt aggccgcggg cgccggcgct tgcttgactt gcccggcctc
     3061 tacgaggata gtcaagtgag tgcttaacca tgtaatcaat cttgatcgga tattccatgc
     3121 cctggccgac ccgacccgcc gcgccatgat cgagcggctc ggccgcgcac cggcttccgt
     3181 gagtgaattg gcgaacccga tgtcagtgtc actggcggcc atcgtgcaac acgtgcagat
     3241 tctcgaagcc agcgggctgg tgcgaaccga gaaagtggga cgggtccgga cttgccggct
     3301 cgacgccggc accttgatgc aggccgagcg gtggctggaa gcacgccgcg gcgagtggaa
     3361 tgcccgcctc gatcggctgg gcgagtatct cgaggagacg acccatccgc cgactccgac
     3421 tccaaagaaa gggcagaagt aaagcaaccc ctgactgaag tcgtcgcggg tttccctgcg
     3481 gagcatctat gaccgtcgcc actcaccacg gacaattcac tctcgagcgc acttatcgag
     3541 ccactccgga gcgggtgtat gctgcgtgga gcgaagtagg cgcgaaggcg cactggttca
     3601 tagggcccga gggctggcgc gagctacggc gcgaaatgga cttccgggtt ggcggacatg
     3661 agttgctgga gggccgttac ggctcggagc gtgtaacccg ctacacggca cgctttcacg
     3721 cgctggaggc cgcgcgacgg atcgtgtttg tttatgacat gcacctcagc gatgtgcatc
     3781 actccacgtc attggcgacc gttgagttgg ctgccactgc caacgggacg cgcctccgtt
     3841 tcaccgagca ggtggccttc ctcgacggca cgacgagcgc cgacagtcgc gcgcacggca
     3901 ctggcgccca tctggacaga cttgcgctat acctcgcgcg cgacaccttc tagccctgcg
     3961 ccgaacgtcc gcgtcggccc ggcgtcgcgc tcatcattac ctgagaggta atcgactcgc
     4021 ctcgggcttg ttcgcatgat ttcttcacta catcgcagtg agagagaatc atgcaaacac
     4081 attacgtccg agcccatgac ggaacgcagc tctactggac agagtggggc tcaggtcctc
     4141 ccattctctt ctcgaatagc gccggcatgc ccaccgaaat gtgggactac cagatgggcg
     4201 ccctcgccga ccatggcttt cgctgcatcg ccttcgatcg acgcggacac ggtcggtccg
     4261 atgttccgcc gcgtgggtac gacttcgaca cgtttgccga cgatatggcg gcgttgatcc
     4321 aggccctgga cctgaagcaa ctcactctga tcgcccactc gatgggtggc ggggaagtgg
     4381 ttcggtatct cacccgtcac ggcaatcagc gcgtatcgcg catcgtgctc atcggctcga
     4441 cgacgccgtt ccagctccag gccgccgaca atccgaacgg aattccagct gccaacttcg
     4501 aagcgctgcg cgcgtcctgg cgcaaggact acccacgatg gatcgccgag aacacggcac
     4561 cgtttttcgt gccggaaacc tcaccggcca tggctcgctg gctctccggg ctgctgatgc
     4621 gcttccccgc tcgtattgcc gtcgccctca acgaggccat tgcgagcgcc gatttcagga
     4681 gggaattgcc gcggatctcg gtacctgcac tcgtcatcca tggtgatcgc gacgtctcgg
     4741 cgccactggc gctgaccggc aagcccactg ctgagctgat accgggctgc cggttcaagg
     4801 tctacgaggg agccccacac ggcctgctct atacgcacat ggaccgactg cacgccgata
     4861 tcctcgagtt catcgctgaa acctgagtgg ctcgccaccg cggcagcggc cagaatggat
     4921 gtgtgtcgaa atcagccccg cccgcgcgtc atcgtgctgg cggagcatgg tgcccgcctt
     4981 cggatcaaag tcggagactc acatgcaata catgctgctg gcatacgcgg acgtcaatca
     5041 gttcctgaag atgacgccgg aggagcagaa acagacgtac ggggcttatc agtcgttcct
     5101 ggaggcggcg aagaaggcgg gcgcatacca ggcctcgggc cgtttgcagc cggcctcgac
     5161 cgcaacctcg gtccgggtat cgaacggcaa gacgcaagtc ctgaatggac cgtacatgga
     5221 gacgaaagag cagcttgggg gtttctttct cctcgatgtg ccggacctgg acgcggcgat
     5281 ctcctgggcg gcgcgctgcc ctgcctccct ccacgggacg gtggaaatcc gcccgatgtg
     5341 gatctcgcag ccctgatcct ggtgtgccgg atcagaccat gacctccggc cggggcggcg
     5401 agcaggcacg tacgatggcg gacgcggtcg cgcgtcgcag ctacggcaag ctcgtcgcct
     5461 ttcttgcggc acgcacgcgc gatgtcgcct cggcggaaga cgcgctctcc gaggcattcg
     5521 cgtcggcgct cgccgattgg cccgtgaacg gctgcccgtc gaatcccgaa ggttggttgt
     5581 tgaccgccgc acgccgcaag ctgatcgatt tcgccaggcg caggcgcacc ggcgagacgg
     5641 ccagtgagcc gctgcggctg ctcgccgagg agttggaaac ggcggcccgt gacaacgagg
     5701 agatggaaat ccccgaccaa cgcctcgcgc tcatgtttgc ttgtgcccac ccggcgatcg
     5761 aggcgagcgt gcgcgcgccg ctcattctgc aggtgttgtt gggtctggat gcggcgatga
     5821 tcgcctccgc cttcctcacc tcaccggcgg ccatgagtca gcgcctggtg cgcgcgaagg
     5881 acaagatccg ccaggccgcc attccgtttc gcatccccga ccgcgaggaa ttgcccggcc
     5941 ggctcggcgc ggtgttggac gcgatctacg ccgccttctc ggagggctgg accgactcgg
     6001 gcggcaca
//
LOCUS       DASRHN010000348        17219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1112175, whole genome shotgun sequence.
ACCESSION   DASRHN010000348 DASRHN010000000
VERSION     DASRHN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17219
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112175"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1342)
                     /locus_tag="VFB37_13975"
     CDS             complement(47..1342)
                     /locus_tag="VFB37_13975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO23606.1"
                     /translation="MSATREDARRVPVPRHKSPPLRGVLASPKMLAILVLGAASGFPN
                     QLTESALQAWLKDAGATNTTIGIMSYVALPYLLKVLWAPLVDRFPLPFLGRRRGWMLA
                     TQVALALCIGLLALQNPVVSLGAVGACALAITFFSATQDIAFDAYRTDVSAPSERGAA
                     AAATNLGYRTAAWVASAFALIVADHFGWRRAFLILAGVMLLFSIATILAPSSQNRYQP
                     RSLRESIVEPVRELLGTPSALVLILLVLVFKAGDAFANKLFTPFMMDVGFSKTEIAVI
                     VKGLFTVSALGGSVLGGVLMIRLGLLRSMLAFGVLQALSNLLYCALAVAGKSYPVMMA
                     AVVIEHIAGAMGGIALVALIMAICDPRYSAFQYALLSAVALLPRYSLGYPAGWIADHG
                     GWYTYYVVSFAIALPGLVIVWWNRRHIDALDRDGAAKPA"
     gene            1473..2132
                     /gene="pyrE"
                     /locus_tag="VFB37_13980"
     CDS             1473..2132
                     /gene="pyrE"
                     /locus_tag="VFB37_13980"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZO23607.1"
                     /translation="MQDYQLAFIDLALARDALRFGHFKLKSGRESPYFFNAGLFSDGE
                     AAALLGRCYAAAITRSGIQFDMLFGPAYKGIPLATVTAVALQEHHGRSVPYAFNRKEA
                     KEHGEGGAVIGGPMKGGVLIIDDVITAGTAVRDSLEIIRVSGGEPVGVALALDRQERG
                     QGELSAVQEIERQQGLRCVSVITLAELIEALSRPRDGRIRISAEQLTSLVAYRQHYGV
                     G"
     gene            2200..2847
                     /locus_tag="VFB37_13985"
     CDS             2200..2847
                     /locus_tag="VFB37_13985"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZO23608.1"
                     /translation="MSARGPRIAGMVIGLAVLMPAAAGAAGSNDKQGVAYRWVDENGV
                     VHYGDQVPPQYAQKQQSVLNSQGVVVRQFDAQKTPEQLAADELKQQSAIRQKQHDSFL
                     LTTYTSVADIEALRDIRLDQLRGQQVAAEQYVENLHSRLTALQGRAKLFKPYSSRPEA
                     RRMPDDLAEDLVRTLNELHTQSGVLVTKGEEEKALRAQFQADIERYRQLHVKHVQ"
     gene            complement(2860..3336)
                     /gene="dut"
                     /locus_tag="VFB37_13990"
     CDS             complement(2860..3336)
                     /gene="dut"
                     /locus_tag="VFB37_13990"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZO23609.1"
                     /translation="MSSAQPAGYRPLKVRILDARLGQEFPLPAYATQGSAGLDLRACL
                     DRALLLEPGKAELIPTGLAIHVEDPGLAAVILPRSGLGHKHGVVLGNLVGLIDSDYQG
                     QLMVSCWNRSREPFTVQPGERIAQLIVVPVVQVELQIVETFAESSRGAGGFGHSGR"
     gene            complement(3333..4559)
                     /gene="coaBC"
                     /locus_tag="VFB37_13995"
     CDS             complement(3333..4559)
                     /gene="coaBC"
                     /locus_tag="VFB37_13995"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZO23610.1"
                     /translation="MQGKRILLGVTGGIAAYKSPDLVRRLRERGAEVQVVMTAGAREF
                     VTPLTFQAVSGRAVRLELWDEAAEAAMGHIELARWAQLVMVAPASADFLARLATGQAD
                     DLLSTLCLATQAPLAVAPAMNHVMWANAATRANVATLQQRGVQVLGPAEGDQACGEVG
                     TGRMLEPVELADRAASLLLGGGPLHERRVLITAGPTRERIDPVRFISNRSSGKMGFAV
                     AQAAREAGAEVILVSGPVSLPTPAGVRRIDVESATEMLTAVLQEVEGADIFISTAAVA
                     DYRPANSAEQKIKKTSDRLDLSMERTPDVLATVAARPGRPFVVGFAAETESVEQNARG
                     KLLKKNLDMIAANEVGHDKAFDCEDNQLVVLWRNGRRELGRASKLTLARELVALIAES
                     YAASRGSVAQRGTARA"
     gene            4842..5594
                     /gene="radC"
                     /locus_tag="VFB37_14000"
     CDS             4842..5594
                     /gene="radC"
                     /locus_tag="VFB37_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZO23611.1"
                     /translation="MALLPCSAITAADALTPQHPIRAERSLTIRDWPKSERPREKLLE
                     QGPAALSDAELLALLLGSGTRGRSAVDVARTLIADFGSLRELLSADRTRWRGRMGIGP
                     ARYATLKAAMELTRRHFREHLRAGSTLNAPDATRRFLIAQLRDRPYEVFCCLFLDNRH
                     RLIAFEEIFRGTIDGASVHPREVIRQTLLHNAASVIIAHNHPSGSLEPSAADEHVTRR
                     LKDALALVDVRVLDHFIVGDGGCFSFAEAGLL"
     gene            complement(5733..5972)
                     /locus_tag="VFB37_14005"
     CDS             complement(5733..5972)
                     /locus_tag="VFB37_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23612.1"
                     /translation="MVTIVDLHQAEELSCSDMAKVAGGGLDLTIPVVVGIGLYGLISS
                     VAGAGLDGAKAANGVSDPETPFPGQSGTLQNLRSP"
     gene            6378..6614
                     /gene="rpmB"
                     /locus_tag="VFB37_14010"
     CDS             6378..6614
                     /gene="rpmB"
                     /locus_tag="VFB37_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZO23613.1"
                     /translation="MTRVCEITGKRPAVGNRVSHANNRKRRRFLPNLHSQRFWVETEK
                     RWVSLRISANGLRTIEKKGIDAVLAELRAQGVKV"
     gene            6646..6801
                     /gene="rpmG"
                     /locus_tag="VFB37_14015"
     CDS             6646..6801
                     /gene="rpmG"
                     /locus_tag="VFB37_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZO23614.1"
                     /translation="MREKIKLLSSAGTGHFYVTTKNKRLHPDKMEVRKFDPVSRKHVV
                     YKETKIK"
     gene            6959..7774
                     /gene="mutM"
                     /locus_tag="VFB37_14020"
     CDS             6959..7774
                     /gene="mutM"
                     /locus_tag="VFB37_14020"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZO23615.1"
                     /translation="MPELPEVETTRRGIEPHAVGRQIVALAVHEPRLRWRVRHDLAEQ
                     VTGQRILHAKRRGKYLLLELQAGTLLWHLGMSGSLRVLPADTPREGHDHVDLILDSGN
                     TLRFNDPRRFGSLLYTTDDPEEHPLLAGLAPEPLESAFDADYLWRITRRRRVAIKQLL
                     MNSRLVVGVGNIYANEALFKAGIRPRRSARSLSRAEVRALVRAVRSVLSRAIRVGGTT
                     LRDYVGADGQPGYFRQKLYVYERAGQPCRVCATRVRQLTQGQRSTYYCPHCQK"
     gene            7783..8526
                     /locus_tag="VFB37_14025"
     CDS             7783..8526
                     /locus_tag="VFB37_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZO23616.1"
                     /translation="MTEPIIAHLTRVDVNMGALIKAYGPYGLIAELDRQPFESLARAI
                     AHQQLHANAARSILQRFVGTCGSGAFPSPQDVMAASTDTLRAAGLSFSKIAALKDLAA
                     KTIDGTVPDSQTLVTLSDLEIIERLTQVRGIGRWTVEMMLMFQLGRPDILPVDDFGIR
                     NGFRHVYGLRKMPAPKVLAAFGERWAPHRTAAAWYLWRALELKKAGKLPHPLERVVLP
                     RLRRRVRRRKVARKKKTAVRTGRRPRARK"
     gene            complement(8492..8887)
                     /locus_tag="VFB37_14030"
     CDS             complement(8492..8887)
                     /locus_tag="VFB37_14030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HZO23617.1"
                     /translation="MSRTDGEVRGLEGAWVPVAANVSGQELRVRELRVKYLVLDGRDY
                     SIIDQSNHIVDRGEYLVDDTSSPQAIDIVGRDGPNAGRSMLAIFELTGDRLTVCYDLD
                     GAERPLNMQAQEDQLLLSITYERAAGVLS"
     gene            complement(8928..9410)
                     /gene="coaD"
                     /locus_tag="VFB37_14035"
     CDS             complement(8928..9410)
                     /gene="coaD"
                     /locus_tag="VFB37_14035"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZO23618.1"
                     /translation="MKRYAMYPGTFDPITNGHHDLVRRAASIFDRVYLAIAANPNKAP
                     MFAMDMRVDLARRVLHDLPNVEIVSYSGLTVEFARKHNVSVVVRGLRAVSDFEFEFQL
                     ANMSRHLAREIETVFLTPQEQFTFISSTLVREIAVLGGDVAEFVHPIVEAELKKQRRV
                     "
     gene            complement(9407..9991)
                     /gene="rsmD"
                     /locus_tag="VFB37_14040"
     CDS             complement(9407..9991)
                     /gene="rsmD"
                     /locus_tag="VFB37_14040"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZO23619.1"
                     /translation="MLRIVAGTWRGRRWRFPPLAGIRPTPDRVRETLFNWLGTRVVGA
                     RCLDLFAGSGALGLEALSRGAVHATFVEREALAVREIRARLAEWGATGARVQHGDALQ
                     YLTGPAPSQLFDTIFLDPPFDSALLSPVARLLEDGRWLAPGALIYVETAAPEGLPELP
                     ESWQIVKAKQAGAVGYHLLERAGRPADEQGAVSA"
     gene            10117..11097
                     /gene="ftsY"
                     /locus_tag="VFB37_14045"
     CDS             10117..11097
                     /gene="ftsY"
                     /locus_tag="VFB37_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZO23620.1"
                     /translation="MFRRSRPAAADAPAASEDRQGFFQRLRARLNRGNSWLTYDLADL
                     FRGRKIDAQLLEELESRLLMADVGIEATEHILSGLQKRLARNELADVAALVGALKSAI
                     VEILAPCAQPLQIDAQREPFVILVVGVNGSGKTTSIGKLARRLADEGHSVMLAAGDTF
                     RAAAIEQLNVWAERTGSEFAAQQAGADPGAVVFDALQSARSRQVGVLLADTAGRLHSQ
                     SHLMEELKKVKRVIQRVDPSAPHEVLLVLDANQGQNALSQAIQFHEAVGVTGLVLTKL
                     DGTAKGGIVVAIARRLQLPIRFIGIGEQAQDFGEFDAEAFATALVEGPKA"
     gene            11094..11786
                     /gene="ftsE"
                     /locus_tag="VFB37_14050"
     CDS             11094..11786
                     /gene="ftsE"
                     /locus_tag="VFB37_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZO23621.1"
                     /translation="MIVFDHVSKRYPNGREALTSVTFEIHTGEMVFLTGRSGAGKSTV
                     LKLIALLERPTRGTVLVSGRNTSTLKSRHIPLFRRQIGVVFQDHRLLADRPVFDNVAL
                     PLVVAGTSLKEVDKRVRAALDQVGLLGKERMLPMELSVGEQQRVGIARAVISKPPLLI
                     ADEPTGNLDPDLALEIMLLFRRFQDVGVTVVVATHDVHVVREFGQRELVIENGQIHDE
                     AADHLPSVIAVR"
     gene            11783..12706
                     /gene="ftsX"
                     /locus_tag="VFB37_14055"
     CDS             11783..12706
                     /gene="ftsX"
                     /locus_tag="VFB37_14055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZO23622.1"
                     /translation="MSMAVAGARHLQALLGAVGKLARNPFSTALTLLVIGLALSLPTA
                     LRLFILNAQLATGGFANAVELSVYLKQDVPLVKAQQLAHNARERSDVASVELIPADQA
                     MQEFRNYSGFGAALDALKENPLPHTLRVQPRSDAQSPADVERLRGYFSAWPEVDIVQV
                     DTDWVKRFNAILDVVRRLLGIVSVLLGAGVLAVIFNTTRLEILNRRAEIEVTKLVGGS
                     NAFVRRPFLYTGALYGLGGALLAWAIVEVAVAVLSQSIATLAQLYGSRFSLQGPSIRD
                     VGILLAAGFLLGWLGAWISAARHLWRIEPRA"
     gene            12927..13805
                     /gene="rpoH"
                     /locus_tag="VFB37_14060"
     CDS             12927..13805
                     /gene="rpoH"
                     /locus_tag="VFB37_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HZO23623.1"
                     /translation="MTSTAIVARPTDLALAGPLGSLDAYLDRVSRIPVLSREEEHRLA
                     ERFRQDADLEAARELVLSHLRFVVHIARGYSGYGLPMGDLIQEGNVGLMKAVKRFDPA
                     VNVRLVSFAVHWIRAEIHEYVLRNWRLVKIATTKAQRKLFFNLRRLKKNLTWLSAEET
                     AAVARDLGVSPSEVTEMEKRLAARDMSFDPTPESEEDEIYSPASYLPAADADPAESVE
                     NAEWEVDSSDRLQEAMGRLDERSRDILQKRWMTDDKSTLHELADKYGVSAERIRQIEA
                     NALAKLRGFIGERAVA"
     gene            14040..15194
                     /locus_tag="VFB37_14065"
     CDS             14040..15194
                     /locus_tag="VFB37_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZO23624.1"
                     /translation="MEIAWPNQSWSLQMKKALWLCALAVPVLACAAPGDEWYVAPEVG
                     AIWPDHNRDLKDRDWLYGVGLGRELGQYLNLELNLNAARINNGRGTGHLDTYGSSLDL
                     LGVLNRGGVVSPYLDVGAGALRDNFFPGNNRTDFMAQTGVGLFLNLWRSPAGTSSFAL
                     RPDIKARWDDPGRGNHWVDYLATVGFQYSFGGSPIAAPVKEALPPPPPPPVVQSAPAP
                     AAQPSPPPDSDHDGVPDSIDQCPNTPPGVQVDAVGCPLKGSITLEGVTFELNSAELTV
                     SSRPALDTMADGLKKHARVKVEIQGHTDSTGAAAYNMKLSQRRADAVRSYLVQAGVNA
                     DQLTTHGYGSTQPVASNATAQGRARNRRVVVHVLDNPGDVKVEGQDTTSP"
     gene            15269..15748
                     /locus_tag="VFB37_14070"
     CDS             15269..15748
                     /locus_tag="VFB37_14070"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HZO23625.1"
                     /translation="MDERLMRSVADWLGEYGASHQNPTNELLHWICVPLIVLSLFGLL
                     WSLPVPAVFASVPWLNWATLMAAAALAYYLMLSPILAIGILISFVVLLLITQWLTMLP
                     WPLWLTSSVIFVTAWIGQFFGHAVEGRRPSFFKDVQFLLIGPLWLLAAAYRRLGLSY"
     gene            complement(15742..17043)
                     /locus_tag="VFB37_14075"
     CDS             complement(15742..17043)
                     /locus_tag="VFB37_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZO23626.1"
                     /translation="MKIPEILLVETIHQPGSLARVLQVIADAGLAIEHLTALRRDQGR
                     TLWEITLEMDEEADRSLYQRIDQLPTARFMGKSDRVFNRHRGGKIRTVASLPINTRQV
                     LRDIYTPGVARVCLAIKNDPQAAHEFTNLDNTVAIVTNGTAVLGLGNIGPLAGLPVME
                     GKAALFGALVGLSGVPILLRETQPDRVVEAITAIETSFGAIQLEDFAAPECFEIEQQL
                     RARIAKPVLHDDQHGTAVVTLAALTNAARQAGRNLEESVVGQVGLGAAGNGIVRLLRA
                     RGVKHVLGADRNAEAVARVVALGGEGATLEQIMARADIVIATTGVKGLIRPEWVRSGQ
                     IILALSNPDPEIEPLIAREHGAAFAADGKGINNVLAFPGLFKGVLSARVSHFSDAMLV
                     AAADAIARLATGDELVPDPLDKTVHESVAAAVHAAALGQAQ"
BASE COUNT         3141 a         5627 c         5354 g         3097 t
ORIGIN      
        1 gacagatgac acgctcgagg tcgatcgctt cggccacggc actcgctcat gctggtttgg
       61 cagcgccgtc gcgatccagc gcatcgatgt gccggcggtt ccaccacacg atcacgagac
      121 ccggtaatgc gatggcgaag ctcactacgt aataggtgta ccagccgccg tgatcggcga
      181 tccaaccggc cggatacccc aggctgtagc ggggcaacag tgccacggcg gacaacagcg
      241 cgtactgaaa tgcgctgtag cgtggatcgc agatcgccat gatcaaggcc acgagcgcga
      301 ttccgcccat cgctccggcg atgtgctcga tgacgactgc ggccatcatg accggataac
      361 tcttgccggc gacggcgagg gcgcagtaca gcagattgct caaggcctgc agcacaccga
      421 acgccagcat cgagcgcagc aggcccagcc ggatcatcag cacgccgccc agcaccgacc
      481 ccccaagggc gctcacggtg aacaaccctt tgacgatcac tgcgatctcg gtcttggaga
      541 agcccacgtc catcatgaac ggcgtgaaaa gcttgttggc gaaagcatcg cccgccttga
      601 agacgagaac gagaagaatg agtacgagcg cgctgggcgt gcccagcagc tcccgaaccg
      661 gctcgacgat tgactcccgc agcgagcgag gctgatacct gttctgcgat gagggtgcga
      721 gaatcgtggc aatcgagaac agaagcatga caccggcgag aatcagaaat gcccggcgcc
      781 acccgaaatg atccgcgacg atcaatgcaa acgcggaggc cacccaggcg gctgtccggt
      841 agcccagatt ggtcgccgca gccgcggctc cgcgctcgct gggtgccgac acatcggtcc
      901 ggtaagcatc gaaagcgata tcctgggtag ctgaaaagaa ggttatcgcg agcgcacagg
      961 cgcccacggc gcccagcgag acgaccgggt tctgcagtgc cagcagcccg atgcacaggg
     1021 cgagcgcgac ctgggtagcg agcatccagc cgcgacgccg tcccagaaag ggcagcggaa
     1081 accgatccac gagcggcgcc cacagcactt tcagcagata cggtaaggcc acatacgaca
     1141 tgatgccgat ggtggtgtta gtcgctccgg cgtccttcag ccacgcttgc agcgcgcttt
     1201 cggtcagttg gttcgggaag cccgaggcgg cacccagcac caggatggcc agcatcttgg
     1261 gactcgcgag taccccgcgt agcggcggcg atttgtgccg tggcacgggc acccgtctcg
     1321 catcttcgcg ggtggcgctc aagtgccccg atccgtcacg gaggttctca tcaggccgat
     1381 actggcatgc catcagttga cagtacaaag gcgcaggtgg caccgtcgcg cgccgaccgc
     1441 acaaagtgcg gaagatcccg cggaaattct gcatgcagga ctaccagctc gccttcatcg
     1501 acctcgcgct ggcgcgcgat gcgctgcggt ttggccattt caagctcaag tccggcaggg
     1561 aaagccccta tttcttcaat gccggactct tcagcgacgg cgaggcggcc gcactcctgg
     1621 gtcgctgcta tgccgccgcg atcacgcgct cggggatcca gttcgacatg ctgttcgggc
     1681 cagcctacaa gggcattcca ctcgccacag tcaccgctgt agcgctgcag gagcaccacg
     1741 ggcgcagcgt gccctacgca ttcaaccgca aggaggccaa ggagcatggc gagggagggg
     1801 cagtgatcgg tggaccgatg aagggcggcg tactgatcat cgacgatgtg atcacggcgg
     1861 gtactgccgt acgcgactcg ctagagatca tccgggtcag cggtggcgag ccagttggag
     1921 tcgcgctggc gctggatcgg caggagcggg gccaggggga gctttccgca gtgcaggaaa
     1981 tcgagcggca gcaggggttg cgctgtgtga gcgtcatcac actcgccgag ctgatcgagg
     2041 cgctctcgcg tccccgggac ggacggattc gcatttctgc cgaacagctc acatcgctcg
     2101 tggcctatcg gcagcactac ggagtcggtt gaccttgcca ggccggctgc gcccggcaga
     2161 atatgtgaca cttcggggtc attcttcaaa ggcaagctca tgtcagctcg ggggccgcgc
     2221 atcgcaggta tggtgatagg gcttgccgtg ctcatgccgg cagcggccgg cgccgccggc
     2281 agcaacgaca aacagggcgt cgcctaccgc tgggtcgatg agaacggcgt ggtgcactac
     2341 ggcgatcagg taccgccgca gtatgcccag aagcagcagt cggttctcaa cagccaggga
     2401 gtcgtggtgc gtcagttcga cgcgcagaag acccccgagc agctcgcggc cgatgagctc
     2461 aagcagcaga gcgccatccg ccagaagcag cacgacagct ttctcttgac gacctacacc
     2521 tcagttgcag acatcgaggc gctgcgggat atccgcctcg atcagcttcg tggccagcag
     2581 gttgcagccg agcagtatgt tgagaatctg cactcgcggc tgaccgcgct gcaaggccga
     2641 gcgaagctct tcaagcccta cagcagcagg cccgaggccc ggcgcatgcc ggacgatctg
     2701 gccgaagacc tggtgcgcac gctcaacgag ctgcacaccc agagcggcgt gctggtcacg
     2761 aagggcgagg aagaaaaggc cctcagagcg caatttcagg ccgacatcga acgctatcgc
     2821 cagttgcacg tcaaacacgt gcaataagcg ctctggccct cagcggcccg agtgcccgaa
     2881 acctccggcg ccgcggctgc tctcagcaaa ggtttcgacg atctgcagct ccacctgcac
     2941 gaccggcacc acgatgagct gtgcaatccg ctccccgggc tggacggtga agggctcgcg
     3001 gctgcggttc cagcacgaga ccatgagctg accctggtaa tcggaatcga tcagtccgac
     3061 caggttgccg agcacgaccc catgtttgtg acccagaccg gagcgcggga ggatgaccgc
     3121 cgccagaccc ggatcctcga catgtatggc gaggccggtc ggaatcagct ccgccttccc
     3181 cggttccagc aacagcgccc gatccaggca ggcacgcagg tcgaggccgg ccgagccctg
     3241 tgtcgcatat gcgggcaacg ggaattcctg gccgaggcgc gcatcgagga tacgtacctt
     3301 gagcggacgg taccccgcag gttgtgcgct gctcatgcgc gcgcggtgcc gcgctgagcg
     3361 acgctcccgc gagaggccgc gtaggactcc gcgatgagcg ccacgagctc tcgtgccaat
     3421 gtcagcttcg aagcccgccc gagctcgcga cgcccgttcc gccacagcac gacgagctgg
     3481 ttgtcctcgc aatcgaaagc cttgtcgtgt cccacctcgt tggccgcaat catgtcgaga
     3541 tttttcttca gcagcttgcc gcgcgcattc tgctcaactg actcggtctc tgcagcgaac
     3601 ccgaccacga agggcctgcc cggacgagcg gccaccgtcg ccagcacatc cggcgtgcgc
     3661 tccatgctca gatcgagtcg atcggaagtc ttcttgatct tctgctccgc cgagttggcg
     3721 gggcgatagt cggccaccgc ggccgtcgag atgaagatgt ccgcaccctc cacttcctgc
     3781 agcacggccg taagcatttc agtggcgctt tcaacgtcga tgcgccgcac accggccggt
     3841 gttggcagac tcacggggcc gctgaccagg atgacctcgg cgcctgcctc acgagccgcc
     3901 tgcgccaccg caaaccccat ctttcccgag ctgcggttac tgatgaagcg aacgggatcg
     3961 atgcgctcgc gggtgggacc cgccgtgata agtacacgcc gctcatgcag tggacctccg
     4021 cccagcagca gtgacgccgc gcggtcggcg agctcaacgg gttcgagcat ccgccccgta
     4081 ccgacctctc cgcaggcctg atcaccttcg gcagggccga gtacctgcac accccgctgc
     4141 tgcagggtcg cgacattcgc ccgggtagcg gcattcgccc acatgacgtg gttcatggcg
     4201 ggcgccacag ccaagggcgc ctgagtggcc aggcacagcg tgctcaacag atcatcggcc
     4261 tgacccgtgg cgagccgtgc cagaaaatcc gcgctggccg gcgcgaccat gacgagctgc
     4321 gcccagcggg cgagctcgat atggcccatg gccgcctcag cggcctcgtc ccacagctcg
     4381 aggcgcaccg cccgaccgga taccgcctgg aatgtgagtg gggtgacgaa ctcgcgcgcc
     4441 ccggccgtca ttacgacctg cacctcggcg ccgcgctcgc gcagcctgcg cacgagatcc
     4501 ggactcttgt acgcggcaat tccccccgtt acccccagaa gaatgcgttt gccttgcatt
     4561 gaaggtctcg ttccctttgt tcgagggatt atcggaccgc cttcgccacg ggcgcaaaca
     4621 acccttcgga atggcagccg tacatttttg ctaaatacct gattcgatag cgcatttttg
     4681 atctcgcaag gcgccctccg ctccaccggc aacccccgtc atgaggaatc cgtcgcgatt
     4741 tcaggcccgt catttcctgg cattctcctg cctttctgcc cctcccccgg ctggctatct
     4801 ctgcgcacac tgcgggtctg caccacgaaa tcccaacccc tatggccctg ttgccatgct
     4861 ccgccatcac cgcggcggat gcgctcaccc cgcagcaccc gatccgtgcg gaacgttcac
     4921 tgaccataag ggactggccg aagtcggagc ggccgcgcga gaagctgctc gaacaggggc
     4981 ccgctgcgct gtcagatgcg gagttgctgg ctctgctgct gggctccgga actcggggcc
     5041 gctccgcagt ggatgtggcg cgcactctga tcgcggactt tggatccctt cgcgagttgt
     5101 tgagtgccga tcggacccga tggcgcggac gaatgggcat cggtcctgcg cgttacgcca
     5161 cgttgaaagc agcgatggaa ctgacacgac gccatttcag agagcatttg cgggccggct
     5221 ccactctcaa cgcaccggat gccacgcgtc gattcctcat cgcccaatta cgcgatcgac
     5281 cttacgaagt cttctgctgc ctcttcctcg acaaccgaca tcgcctcatc gccttcgaag
     5341 agatcttccg gggcacgatc gatggtgcaa gcgtgcatcc gcgcgaggtt atccgtcaga
     5401 cgctgttgca taacgcggca tccgtgatca tcgcccacaa tcatccttcc ggttcgctcg
     5461 aaccgagcgc cgccgatgag catgtcacgc gacgactgaa ggatgccctc gcgctcgtgg
     5521 acgtccgcgt tctcgatcac ttcatcgtgg gcgatggtgg ctgcttctcg ttcgccgaag
     5581 caggccttct ttgacatgtg tcgcggctgc tgaatgaggc tgcaacgggt ccatcgtatc
     5641 ccgacgcgaa gcgcgaaggg ttgagcgacc ggcagagaaa aaagggcgca gcacgagcta
     5701 ctgcgccgcg taaagttcaa ttccggactg cttcacggac tcctgaggtt ttgtaacgtg
     5761 ccggactgac caggaaaagg agtctccgga tcagacacgc cgttcgccgc tttcgctcca
     5821 tccagaccag ctcccgcaac gctggaaatc aggccgtaga gaccgattcc gaccaccaca
     5881 gggatggtga gatccaagcc gccgccagca actttggcca tgtcggagca cgaaagctcc
     5941 tctgcctgat gcagatcaac gatagtcacc ataacgatac tcctgagaga ttgagttgtt
     6001 tgagcattgc tcgaaccgaa gcgtgtctca gggtccgggt ttgcgacagt gcccttggtc
     6061 atcgcgtgac ccactacatg tgtgttgccc gcacgcgtgg cagtttggcg aggagttcga
     6121 tgacctcatc cagattggcc gggtgcccgg ccaatggatt gggcaaggtt cgctccaggc
     6181 cgtgacgggt gcaggaacct gtcgtcggtt gcggcaaacc gatgcgccgg ggcatctggc
     6241 ggtcgagggt agtttgagta tccggtccag ccgcagcttg tgatcccggg accgggctgg
     6301 tataaagcgc gactcggcgg gatgggtatc cggccccccg acgcagtcta atttcctgtt
     6361 tttccaaggc atcacgtatg acccgcgtct gcgagatcac aggcaagcgc cccgctgtgg
     6421 gcaaccgcgt ctcccacgcg aacaacagga agcgccgccg cttcctgccg aacctgcatt
     6481 cacagcgctt ctgggtcgag accgaaaagc gctgggtgtc gctgcgcatc tcagccaatg
     6541 gcttgcgcac catcgagaag aagggcattg atgccgttct cgccgagctt cgcgcccagg
     6601 gcgtgaaggt ctgaatccag gcgcccgtcg cgataaggag tccccatgcg cgagaagatc
     6661 aagctgctgt cctcggccgg taccggacac ttctacgtaa ccacgaagaa caagcgcctg
     6721 cacccggaca agatggaagt gcgtaagttc gatccggtgt cacgcaagca cgtggtctac
     6781 aaggaaacga agatcaagta gaccgggcag cgggccccca tatcgggctc cgtccgcgtg
     6841 agtgcgggca ccggcagcgc ccgccggtaa gtggcgcccc caggcagaca accccgccga
     6901 cccaggcgtg agcctcttgc gttttggcct gcagagggcg aatgcttccg gcctctgtat
     6961 gccagagctt cccgaagtcg aaaccacgcg ccgtggcatc gagccgcacg cggtaggccg
     7021 gcagatcgtc gccctcgccg tgcacgaacc tcgtttgcgg tggcgagtcc gccatgacct
     7081 ggccgaacag gtgactggcc agcgcatcct tcacgcgaag cggcgcggaa aatacctttt
     7141 gctcgaatta caggccggca cactgctgtg gcatctgggc atgtccggta gtttgcgcgt
     7201 gctgccggcc gacaccccgc gggaggggca tgatcatgtc gatctgatcc tcgactccgg
     7261 caacacgctg cggttcaacg atcctcgccg cttcggcagc cttctctaca ccacggacga
     7321 cccggaagag cacccgctgc tcgccggcct cgcgcccgag ccgctcgagt ccgccttcga
     7381 cgccgattat ctatggcgca tcacgcgtcg caggcgggtc gcaatcaagc agctgctcat
     7441 gaacagtcgg ctcgtcgtgg gggttggcaa catctacgcc aatgaagcgc tgttcaaggc
     7501 cggaatacgc ccacggcgat ccgcgcggag cctgtcccgg gccgaggtgc gggcgctggt
     7561 gcgcgcggtg cgttccgttt tgtcgcgggc aatccgtgtc ggaggtacga ccctgcgcga
     7621 ttatgtcgga gcggacgggc aacccggtta cttcagacag aagctgtatg tttacgaacg
     7681 ggccggccaa ccgtgcagag tctgtgcaac gagggtgcgt cagctcacgc agggtcaacg
     7741 atcgacctac tactgtcccc actgtcagaa gtaagggctc cgatgactga accgatcatc
     7801 gcgcatctga cccgtgtcga tgtcaacatg ggtgcgctca tcaaagccta cggaccttat
     7861 gggctgattg cggagctgga ccgacaaccg ttcgagtcgc tcgcgcgggc catcgcgcac
     7921 cagcaactgc acgcgaacgc ggcgcgaagc attctgcagc gcttcgtcgg cacctgtggc
     7981 agcggcgcat ttccctcgcc gcaagatgtc atggccgcat cgacagacac gctgcgcgca
     8041 gcggggctgt cattttccaa gatcgccgcg ctcaaggatc tggcagccaa gaccatcgac
     8101 ggcaccgtac cggacagtca gacgctggtc actctcagcg atctcgagat catcgaacgg
     8161 ctcacgcagg tgcgaggcat cggccgatgg accgtggaga tgatgctcat gtttcagtta
     8221 ggtcgtcccg atatcctgcc ggtcgacgac ttcggcattc gcaatggctt tcgccacgtg
     8281 tacgggttga gaaaaatgcc cgcgccgaaa gtgctggcag cgttcgggga gcgatgggct
     8341 ccgcatcgca cggcggccgc ctggtatctc tggcgggcac tcgagttgaa gaaggcgggg
     8401 aagctgcctc atccgctgga gcgcgtcgtg ctgccacgcc tgcgccgcag ggtgcggcgt
     8461 cgcaaggtgg cgcgcaaaaa gaaaacagcc gtcaggacag gacgccggcc gcgcgctcgt
     8521 aagtgatgct gagcagcagc tgatcctcct gagcctgcat gttgagcggg cgctctgcgc
     8581 catcgaggtc atagcacacg gtgaggcggt cgccggtgag ctcgaagatc gccagcatgc
     8641 tgcggccggc attgggtccg tcacgtccga cgatgtcgat cgcctgcggg ctgctcgtgt
     8701 catccacgag atactcgccc ctatccacga tatggttgct ctgatcgatg atgctgtagt
     8761 cgcgcccgtc gagcaccaga tacttgacgc gcagctcgcg cacgcgcaac tcctgtcccg
     8821 acacattggc cgcgacggga acccacgcgc cctcgaggcc gcggacttca ccatctgtcc
     8881 tggacactct gctcattgcg ctgcgatacc cgctggggag acaaccctca aacgcgtcgc
     8941 tgctttttca gttccgcttc aacgatcgga tggacgaatt cggccacgtc accgcccagg
     9001 accgcgatct cacgtaccag agttgaagag atgaacgtga actgctcctg gggtgtgaga
     9061 aagaccgtct cgatctcccg ggcgagatgg cggctcatat tggccagctg aaactcgaac
     9121 tcgaaatcgg ataccgctct caatccgcgc acgaccaccg agacgttgtg cttgcgcgca
     9181 aactccacgg tcagcccgga atagctgacg atctccacgt tcggcagatc gtgcagcacg
     9241 cggcgcgcca aatccactcg catgtccatt gcgaacatgg gtgccttgtt gggattggcg
     9301 gcgatggcga ggtagacgcg atcgaagatg ctggctgcgc ggcggaccag atcgtggtga
     9361 ccgttggtga tcgggtcgaa tgtccccgga tacatcgcat agcgtttcat gcgctgaccg
     9421 ccccctgctc atctgccggc cgcccggcgc gctccagcag atgatacccg accgccccgg
     9481 cctgcttcgc tttgacgatc tgccagctct cgggcagctc cggcaggccc tcgggggctg
     9541 ccgtctcgac ataaatcagc gctccgggag cgagccaccg tccatcctcg agcagccggg
     9601 cgaccgggct cagcagggcc gaatcaaacg gcggatcgag gaaaatcgta tcgaataact
     9661 gcgaaggggc aggcccggtg aggtactgca gagcgtcccc gtgctgaact ctcgcgccgg
     9721 tcgctcccca ctccgccagc cgcgcccgaa tctcgcgtac ggccagcgcc tcgcgctcca
     9781 cgaacgttgc atgcacggcg cccctcgaaa gtgcctcgag gccgagcgcg ccgctgccgg
     9841 caaagaggtc caggcatcga gcgcccacca cgcgtgtacc cagccagttg aacaacgtct
     9901 cccgcacgcg gtcgggtgtc gggcggatgc cagccagggg cggaaatcgc cagcgcctgc
     9961 cgcgccacgt tccagccact attcgcaaca tgcgcgagtt cgcagcacga gagggcatgc
    10021 gccccgtggc gggggccgat tttccgattt ttgtcaacct ggtgccgctt ccgccagccg
    10081 cttgcctctg ttctcgcggc aagcctaccc taccccatgt tcagacgctc acgccccgcc
    10141 gctgccgatg ctccagccgc gagtgaagat cgacagggtt tcttccagcg tctgcgcgcc
    10201 cgcctcaacc gcggcaactc ctggctcact tatgacctgg cggatctgtt ccgcggccgc
    10261 aagatcgatg cacagctcct cgaggagctc gagagccgtc tgctgatggc ggacgtggga
    10321 atcgaggcga ccgagcacat cctctcgggc ctgcagaagc ggctcgcacg taacgagctg
    10381 gcggacgtcg ctgcgctcgt cggcgctctc aaatcagcca tcgtcgagat tctggcgcct
    10441 tgcgcgcaac cgctgcagat cgatgcccag cgggagccgt ttgtcattct cgtggtgggc
    10501 gtcaacggat cgggcaagac cacgagcatc ggcaagctcg cacggcggct cgcggatgag
    10561 ggccacagtg tcatgctggc ggccggcgat acgttccgcg ccgccgccat cgagcaactc
    10621 aatgtctggg cggagcgcac gggctcggaa ttcgctgctc agcaggcggg agccgatccg
    10681 ggcgcggtgg tattcgatgc tctgcagtcc gcccgctcgc gccaggtcgg cgtgctgctc
    10741 gcggacacgg ccggccgcct ccacagccag tctcacttga tggaggagct caagaaggtc
    10801 aagcgtgtca ttcaacgcgt cgatccgtcc gctccccatg aagtgcttct cgtcctggat
    10861 gccaaccagg gacagaacgc gctgtcgcag gccattcagt tccacgaagc ggtcggcgtc
    10921 accggactcg ttctgaccaa gctcgacggc acggcaaaag gcgggatcgt cgtggctatc
    10981 gcgagacgcc tgcagcttcc catccgcttc atcggcattg gcgagcaggc gcaggatttc
    11041 ggcgagttcg atgccgaggc attcgctacc gccctcgtcg agggacccaa ggcatgatcg
    11101 tctttgacca tgtcagcaag cgctatccaa acggccgtga ggccctgacc agtgtcacgt
    11161 tcgagatcca taccggagaa atggtgtttc tgaccggtcg ctccggtgcg gggaaaagca
    11221 cggttctgaa gctcattgcg ctgctcgagc gcccgacgcg cggtaccgtt ctcgtcagtg
    11281 gacgcaacac cagcacactc aagtcgcgcc acatcccgct cttccgccgc cagatcggcg
    11341 tggttttcca ggaccatcgc ctgctcgccg accgacccgt cttcgacaac gtggccttac
    11401 ccctggtcgt cgcgggcacc tcactgaagg aagtcgacaa gcgtgtccgc gcggcgctgg
    11461 atcaggtggg acttctcgga aaggagcgca tgctccccat ggaactgtcc gtaggtgagc
    11521 aacagcgggt cggcatcgca cgcgccgtaa tcagcaagcc ccccctgctc atagcagacg
    11581 agcccacggg caacctcgac ccggatctcg cgctcgagat catgctcctg ttccgccgat
    11641 ttcaggatgt cggcgtgact gtcgtggtgg cgacgcacga cgtccacgtc gtgagggagt
    11701 ttggccagcg tgagctcgtg atcgagaacg ggcagataca cgatgaggca gcggatcatc
    11761 tgcccagcgt gatcgcggtt cgatgagcat ggccgtggcc ggcgcccggc atctgcaagc
    11821 tcttctcggc gccgtcggca agctggcgcg caacccgttc tccaccgcgc tcacgctgct
    11881 ggtgatcggg ctcgccctct cacttcccac cgcattgcga ttgttcatcc tgaacgcgca
    11941 gctggcaacc ggagggttcg ccaatgccgt cgagctctct gtctatctca agcaggatgt
    12001 gccgctggtc aaggcccagc aacttgctca taacgctcgc gagcgctcgg acgtggcctc
    12061 cgtcgaactc atacccgccg accaggcgat gcaggaattc cgcaattact cgggtttcgg
    12121 tgcggccctc gatgcgctca aggaaaaccc actgccccac acgcttcggg tccagccgcg
    12181 cagcgatgct cagtctccgg ccgacgtgga gagactgcgc ggctacttca gcgcgtggcc
    12241 ggaagtggac atcgtgcagg tggacactga ctgggtgaag cgcttcaacg ccatcctcga
    12301 tgtggtgcgc aggctgctcg ggatcgtctc ggtgctgctg ggcgcgggtg tactcgcagt
    12361 gatattcaac accacgaggc tcgagattct gaaccgccga gccgagatcg aggttacgaa
    12421 gctggtaggc ggctccaatg cgttcgtgcg tcgcccgttc ctctataccg gggcgctcta
    12481 tgggctcggg ggtgcgctgc tcgcctgggc gatcgttgaa gtcgccgtgg cggtcctcag
    12541 ccagtcgatc gccacgctgg ctcagctgta cggcagccgg ttctcccttc aggggccgtc
    12601 gatccgcgat gtcgggatcc tgctggccgc cgggtttctg ctgggctggc tgggagcctg
    12661 gatttccgcc gcgcgccacc tgtggcgcat cgagccgcga gcctaactgg ccgaaccgtc
    12721 tctggttggg ctgcatagct caagtctgga accggtcccc ggatcatgcg ttgccactcc
    12781 tttgggagag gctgccctgc ccctcccggt ccactgataa gcctctgttt ctcaaggtaa
    12841 actccggggg accgtgcgga acttcctgac cccttagcac tccaatagct cgactgctag
    12901 aatccccatc cattctggga gacctgatga ccagtaccgc aatcgtcgcc cgtccgaccg
    12961 atttggcctt ggccggcccg ctgggaagcc tcgatgctta cctggaccgg gtgagccgca
    13021 ttcccgttct cagccgtgag gaggagcatc ggcttgccga gcgttttcgc caggatgccg
    13081 atctggaggc agcgcgcgag ctcgtgctct cgcatctgcg cttcgtggtc cacatcgcgc
    13141 gcggctacag cggctacgga ttgccgatgg gcgatctcat ccaggaaggc aacgtcggcc
    13201 tcatgaaggc ggtgaagcgg ttcgatccgg ctgtcaacgt ccgcctggta tcctttgccg
    13261 tgcactggat tcgagccgag attcacgagt acgtgctgcg caactggcgt ctcgtgaaga
    13321 tcgccacgac caaggcgcag cgtaagcttt tcttcaacct gcgtcggttg aagaaaaacc
    13381 tgacgtggct gtcggctgaa gagaccgccg cggtggcccg cgatctcggt gtcagtccga
    13441 gcgaagtcac cgagatggaa aagcggctcg ccgcccgcga catgtcgttc gatccgacgc
    13501 ccgagtccga agaagacgaa atctatagcc cggcttcgta tctgccggca gccgatgccg
    13561 atccggcgga gagtgttgag aacgccgagt gggaagtcga ttcctccgac cgcctccagg
    13621 aggcgatggg tcggctggat gagcgcagcc gggatatcct gcagaaacgt tggatgaccg
    13681 acgacaagtc aacactgcac gagctcgccg acaagtacgg cgtttcggcc gagcgcattc
    13741 gccagatcga agccaatgca ctggccaagc tgcgcggctt catcggcgag cgcgccgtgg
    13801 cttgatttag cccttcacct taaaaaatag aagcgaggtc ccgaagcagg aaagcttcgg
    13861 gtctcgcgct tgcgcatgaa aagcaccgcc gagctcgcgt agtttgcgct ccgggcacag
    13921 ccgctgcgtc tacctccacg cttgcagcct cgcgacgaca atgggaacgt ggttcgacca
    13981 gctgtatttc cagcccctcg tggccccatg gcagttcgcg ttgcagatct gcaacgccca
    14041 tggagatcgc ctggcccaat caatcatgga gtctgcagat gaagaaagcg ctctggctgt
    14101 gtgctctggc cgtcccggtg ttggcttgcg ccgctcccgg tgatgagtgg tacgtcgctc
    14161 ccgaggtggg tgccatctgg cccgaccaca accgggatct gaaggaccgc gactggctct
    14221 acggcgtcgg actcggccgc gagctcgggc agtacctcaa tctggagctg aatctcaatg
    14281 ccgcgcggat caacaatggc cgcggcacgg gccacctgga tacctacggc agttccctcg
    14341 atctgctcgg cgtgctcaac cgcggcggag tcgtctcgcc ttacttggat gtcggtgccg
    14401 gcgctttgcg cgataacttc ttcccaggca ataaccgaac agatttcatg gcgcaaaccg
    14461 gggtgggcct gttcctgaat ctgtggcgca gccctgccgg caccagctcc tttgcactgc
    14521 ggcctgacat caaggcgcgc tgggacgatc ccggccgcgg gaatcactgg gtggactacc
    14581 ttgcgaccgt cgggtttcag tactccttcg gcggcagtcc aattgctgct ccggtgaagg
    14641 aggcgctgcc cccgccgccg cccccgccag tggttcaatc tgcgcccgcg ccggctgcgc
    14701 agccaagtcc tccgccggat agtgatcacg acggcgtccc cgacagtatc gatcagtgcc
    14761 ccaacacccc gcccggcgtc caggtggatg cagtgggatg tcccctcaaa ggctcgatca
    14821 ctctggaagg cgtgactttc gagctcaact cggcagagct gacggtctcc tcgcgtccgg
    14881 cactcgatac gatggccgat ggactgaaga aacacgcgcg agtgaaggtg gagatccagg
    14941 gtcacaccga cagcaccggt gcggccgctt ataacatgaa gctctcgcag cggcgcgcgg
    15001 acgcggtgcg cagctatctc gtgcaggcag gcgtcaatgc cgatcagctc acgacccacg
    15061 gctatggttc gacgcagccg gtcgcgagca acgcgaccgc gcaaggtcgg gccaggaacc
    15121 gccgcgtggt cgtgcatgtc ctcgacaatc caggtgatgt gaaggtcgaa gggcaggata
    15181 cgacttcgcc ttgagtcccc agcctcggcg ggcgcggccg cttgcggctg cgcaccgccg
    15241 ggcgtagcgt cccctgaagg tgctggcgat ggacgaacgg ttgatgcgca gcgttgcgga
    15301 ctggctcggc gagtatggcg caagccatca aaaccctacc aacgagctgc ttcactggat
    15361 ctgcgtgccg ctgatcgtgc tctcgctgtt cgggctgctc tggtcgctgc ccgtgcccgc
    15421 agtgtttgcg agtgtgccct ggctcaactg ggcaacgctg atggcggccg ctgcgctcgc
    15481 gtactacctg atgctgtcgc caatcctggc gatcggcatt ctgatctcgt tcgtggtcct
    15541 gctgctcatc acgcagtggc ttacgatgct gccctggccc ttgtggctca cctcgagcgt
    15601 gatattcgtg accgcgtgga tcggtcagtt cttcggccac gcggtcgaag gcaggcggcc
    15661 ctccttcttc aaggatgtgc agttcctgct catcggacca ttgtggctgc tggcggccgc
    15721 gtatcggcgg ctgggcctga gctactgagc ctgccccagc gcagcggcat gcacggcggc
    15781 cgcgacactt tcgtgcacag tcttatccag cgggtctgga accagctcat ctccggtggc
    15841 aagccgcgca atggcgtctg cagctgcgac cagcatcgca tccgagaagt gggagacgcg
    15901 ggcgctgagg actcccttga acagccccgg aaacgcaagc acgttgttga tcccctttcc
    15961 gtcggccgca aaagccgcgc cgtgctcgcg agcaatcagt ggctcgatct ctggatccgg
    16021 attcgacagc gccaggatga tctggccact tcgcacccac tcgggacgaa tgaggccctt
    16081 gacgcccgtc gtagcgatca cgatatccgc gcgcgccatg atctgctcga gggtcgcacc
    16141 ctcgccgccc agagcaacca cacgcgctac ggcttctgca ttgcggtcgg cgcccagcac
    16201 gtgtttgacg ccgcgcgccc gcagcagccg cacgatccca ttgcctgcgg cgcccagtcc
    16261 cacctggccc accacgcttt cctccaggtt gcggccagcc tgccgcgcag cattggtcag
    16321 cgcggcgagc gtcaccaccg ccgtgccatg ctgatcgtcg tgcaggacgg gtttggcgat
    16381 ccgcgcgcgc agttgctgct cgatctcgaa acactcgggc gctgcgaaat cctcgagttg
    16441 aattgcccca aaggaggtct cgatggccgt gatggcctcc accactcggt caggctgggt
    16501 ttcccgcagc aggatcggca cgccgctcaa gcccacgagt gcgccgaaca gagccgcctt
    16561 accctccatg acgggcaggc ccgcgagggg cccgatgttg ccgagcccca gcacggcggt
    16621 gccgttggtc acgatcgcca cagtattgtc gagattggtg aactcgtgcg cggcctgcgg
    16681 atcgttcttg atcgccagac atacacgcgc cacgcctggt gtgtagatgt cgcgcaacac
    16741 ctgcctggtg ttgatcggaa ggctcgcgac cgtccggatc ttgccgccac gatggcgatt
    16801 gaagacgcgg tcggacttgc ccatgaagcg cgcggtcgga agctgatcga tgcgttgata
    16861 gaggctgcga tcggcctcct cgtccatctc cagcgtgatc tcccatagcg tgcggccctg
    16921 atcgcgccgc aatgccgtga gatgctcgat ggccagcccc gcatcagcga tcacctgcag
    16981 cacgcgggca aggctgccag gctgatggat cgtctcgacg agcagaattt cgggaatctt
    17041 cacgcgcgcg cctctttcct gtgcgtgcaa tagtaatcct gaatacggcg gcccggaggc
    17101 cctccggggg agcatggttt atgatgtgga ttgcccatct tattgcgaat gccggagagt
    17161 agcgtggcca agcgttcccc agcgaagctg gatcccctgg accttttcga tgtccgcgg
//
LOCUS       DASRHN010000349         5538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_457330, whole genome shotgun sequence.
ACCESSION   DASRHN010000349 DASRHN010000000
VERSION     DASRHN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457330"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..459
                     /gene="pqqD"
                     /locus_tag="VFB37_14080"
     CDS             178..459
                     /gene="pqqD"
                     /locus_tag="VFB37_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HZO23627.1"
                     /translation="MKDKPPSRPGRVRLAPGRQLQKDSAGTGQLLVGPGGIVQLNESA
                     AAILALCDGSLTREQIVARMLPRSKDDSFAEDVREFLNAARRRGWIVEE"
     gene            721..972
                     /locus_tag="VFB37_14085"
     CDS             721..972
                     /locus_tag="VFB37_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23628.1"
                     /translation="MRPLLSVCELVDEEELVELGLVALLYGGLVLAPGVAGAGVPRIV
                     VPRKSLPFADDPGVGLGLAGVPRMVVFCGGGVAAGWLLC"
     gene            991..1188
                     /locus_tag="VFB37_14090"
     CDS             991..1188
                     /locus_tag="VFB37_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23629.1"
                     /translation="MPGAVFMLGGSMEGDREEEPGGTAELEDEDGRVPEELPPELPVV
                     DCDCAWAAAALASAKVTARAM"
     gene            1369..2547
                     /locus_tag="VFB37_14095"
     CDS             1369..2547
                     /locus_tag="VFB37_14095"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZO23630.1"
                     /translation="MSASSNAEHRAARENESEPGRREGQSAGDEGRSDGEKGQSSGGK
                     ERGPGKPSLFRRPAVLIGGGIVLLLLILTGALWWWHASGFENTDDAFIDARTVRVAPR
                     ISGQVIRVLVTDNQAVHRGDVMVEIDPADTQLALDQATAQQSQAETALGQAQADVEVA
                     EAQRRQALATASGADAQALNAQQDLRRYRDLKASTPQAVAQGQLDQATAAARNASAQR
                     DAAHQQIQGAEAQITAARAAVSGAEARLQTARAQVRQAELNLGYTRVLAPVDGHVARR
                     TVAVGSYVAPGQELLAIVPLHLWVTANFKENQLVRIRPGQSVTVHVDACPAVDLDARV
                     DSIQRGAGQAFALLPAENATGNFVKVVQRVPVKIELEDLPRNCPLGPGMSVEPSVRVR
                     "
     gene            2549..4132
                     /locus_tag="VFB37_14100"
     CDS             2549..4132
                     /locus_tag="VFB37_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO23631.1"
                     /translation="MAAGWSAERSAAGSHSPWLIASVISISAFMEVLDTAIANVALRH
                     IAGSTSSSYDEATWVLTSYLIANAIIIPMSGFFADVIGRKRYYMMSVALFSIASLCCG
                     LAPTLPLLIAARIVQGIAGGGLQPITQAMLVDTFPPERRGQAMAMYGLTIILAPTIGP
                     LLGGAITDHFSWHWVFLINVPIGALSLFLVQALVNEPEVLQKERAQRLKRGIRLDVPG
                     AVLIAMGLAFLEVTMDRGEREDWFASPLISISAVIAGLSLISFAVWEWFQKEPLLDMK
                     LFRNRNFAVANLVIMIVGVILFGTTQFIPQMLQEVLGYTATAAGEALTLGGAVTLIAM
                     PIAGVLSGKVQPRVLMGCALIVEVLALWNMTGFATTMSMHDAAMARLWQALGIPFLFV
                     PLTSAAYVGLPPHRSNQGSAMLNVARNLGGTMGISLVQALLARREQFHQMRLVEHLEP
                     LNPSYAAGLHSLTQTLTSQGLPPAAANQSALGLIYQTVLQQASMQAFIDCFWILMLFV
                     VLVIPTVFFLKRTPLVHES"
     gene            4174..>5538
                     /locus_tag="VFB37_14105"
     CDS             4174..>5538
                     /locus_tag="VFB37_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO23632.1"
                     /translation="MSTLAGCTVGPDYRTPQVAVPDQYERVPGSSVEAPLSQPLPSEV
                     DLSQWWQQFHDSQLDDLITRALKANLDLQTAASRIRQAREQEIIAGAARLPSVSATGL
                     AARLHTNSNPLAALSGGQSGGSQSGGGASGPSGATLDLYSIGFDATWEADIFGGVRRG
                     IEAARANTEAAEWQMRDAQVSLSAEVANGYLGLRTTQTRLAILQQSAQHQQELLQLAS
                     ARARTGFVTELDVNQQRTQLAATIAQVPTLQAEERAQVHALGVLLGEAPEALTGELAG
                     EATAPAVPTELPVGLPSELLRRRPDVRRAERQLAAATAQVGVAVATLYPKFNLIGAVS
                     LASTSTGKLFDSSSLGNFGAGMISWPIFQAGKVRANVHANEEQQKQAYLAYRKSVLVA
                     LQDAEDALVRYAAEQRRLVSLRQSLAAAASSRHIAEEQYRTGLVTFINVLSATTTELT
                     AQDEV"
BASE COUNT          904 a         1777 c         1860 g          997 t
ORIGIN      
        1 taccgccgca tgaagcgtca cgccatcccc ctcgcacacg ggggtggatg agggaaagcg
       61 ggcacgttga accggctctg gcccggacct tgcacgcacc gatcgcgcgc gcgggcacct
      121 cgcgcatgat cgaccgttcg cggggcgggc agtgtcctcg cgtggtggag ccagggcatg
      181 aaggacaaac caccgtctcg tcccggcagg gttcggctcg ccccgggacg ccagttacag
      241 aaggactccg ccgggaccgg ccagctactg gtcggacccg gcgggatcgt tcagctcaat
      301 gaaagcgctg cggccatcct cgcgctatgt gatggctcgt tgacccgcga gcagatcgtc
      361 gcccgcatgc tgccccgctc gaaggacgac agtttcgcgg aggacgtccg cgagtttttg
      421 aacgccgcca gacggcgggg ttggatcgtg gaggagtaaa cccctgcgat cccatcagcc
      481 tcaatgtcgt tcgggctcga agaggccgat cttccgcaag cccattcacc tcaactgccg
      541 tgcgggctcg acgaggagct tgacggggcc ggttgactcg agctgctcgg cgcgctcgag
      601 gagctgctcg gattcgtcgt actgctgctc gagctctgcg agcccgggac gggcgcgctc
      661 actgacgggc tgatcgagga ggcattgccg ttcgtattgg ccggcgcctg ggcgctgcga
      721 atgcggccgc tgttgtccgt ctgcgaactc gttgacgagg aggagctcgt ggagctcggg
      781 ttagtcgcgc tgctgtacgg cgggctggtg cttgcgccag gcgtcgccgg agcaggcgta
      841 cccagaatcg tggtcccgcg caagtcattg ccgtttgccg atgatcctgg cgtcgggctc
      901 ggtttggccg gcgttccaag gatggttgta ttctgtggcg gcggcgttgc ggccggctgg
      961 ttgctctgtt gagtcggcgg cggagtcatc atgcccgggg ccgtgttcat gctgggaggc
     1021 agcatggagg gggaccggga agaggagccg ggaggcacgg ccgagctcga ggacgaggat
     1081 ggcagggtcc cggaagagct gccgcccgag cttcccgtcg tggactgtga ttgcgcctgg
     1141 gctgcggcgg cactggcaag tgccaaggtt accgcgaggg ctatgtaact caatttactc
     1201 atattgatct ccttgcctct cagggagaag ccgcaatcgc catacctgcg ccggccctgt
     1261 tcccccccgt gaaacccttc tctttgcagt gaacggtgcc ggttcaccag gcgcccgctg
     1321 ctgtcaccca ccggaacaaa agcgcttccg tgaccgttgc cctgaaccat gtccgcatca
     1381 tcgaatgcag agcatcgggc agcgcgcgaa aacgaaagtg agccgggcag gcgcgaaggt
     1441 cagtcggccg gcgacgaagg ccggtcggac ggcgaaaaag gccagtcctc cgggggcaaa
     1501 gagcgtgggc ccggaaagcc ttccctgttt cgccgccccg ccgtgctgat cggcggcggc
     1561 atcgtcctgc tgctcctcat cctcaccggc gcgctctggt ggtggcacgc ttcgggtttc
     1621 gagaacaccg acgacgcttt catcgatgcg cgcacggtac gtgtggcgcc gcgcatcagc
     1681 ggacaagtga tccgcgtgct ggtcacggat aaccaggccg tgcaccgcgg cgacgtcatg
     1741 gtcgaaatcg atccggcgga cacgcagctc gcgctcgatc aggccactgc ccaacaatcc
     1801 caggccgaga cggcgctcgg ccaggcgcaa gccgatgtgg aagtcgccga ggcacagcgc
     1861 aggcaagctc tggcgacggc ctcgggcgcg gacgcccagg cgctgaatgc gcagcaggat
     1921 ctgcggcgtt accgtgatct gaaagcttcc acgccgcaag cggtcgcgca gggccaactg
     1981 gatcaggcga ccgccgcagc tcgcaacgcg agcgcgcagc gcgatgcggc gcatcagcag
     2041 atccagggcg ccgaagccca gatcactgca gcgcgggcgg ccgtgtcggg ggccgaggcg
     2101 cggttgcaga cggcccgtgc ccaggtgcgg caggccgagt tgaatctggg ctacacccgg
     2161 gtgcttgctc ctgtcgatgg gcacgtcgcg cgccgcacgg tcgccgtagg tagttacgtt
     2221 gcacccggac aggagctgct cgcgatcgtg ccgctgcacc tgtgggtcac cgccaatttc
     2281 aaggaaaacc aactcgtgcg gatccgacca ggccagagcg tcacggtgca cgtcgatgcc
     2341 tgtcccgcag tcgatctgga tgcgcgcgta gattcgattc agcgcggcgc cgggcaggcg
     2401 ttcgcgctgc tgcccgcgga gaatgcgacc ggcaatttcg tcaaggtcgt gcagcgggtg
     2461 cccgtgaaga tcgagctcga ggatctgccg cgcaactgtc cgctcgggcc ggggatgtct
     2521 gtcgagccga gcgtccgggt gcgctgacgt ggctgcaggt tggtcggccg agcgatcggc
     2581 ggcaggcagt cacagcccgt ggctgatcgc ctcggtcatc tcgatttccg ccttcatgga
     2641 agtgctggat acggccatcg ccaacgtggc cctgcgccac atcgcaggtt cgacctcatc
     2701 cagttatgac gaggcgacct gggtactcac gagctacctg atcgccaatg caatcatcat
     2761 tccgatgagc gggttcttcg ccgacgtcat cggacgcaag cgctactaca tgatgagcgt
     2821 cgccctgttc tccattgcat cgctctgctg tgggctcgcg ccgacgctgc cgctgctgat
     2881 cgcggcgcgc atcgttcagg gcatcgccgg gggcggcctg cagccgatca cacaggccat
     2941 gctggtcgat acttttccgc ccgagcgccg cgggcaggcc atggcgatgt atggcttgac
     3001 gatcattctc gcgccgacga tcggccccct tctgggcggt gccatcaccg accatttctc
     3061 ctggcactgg gtcttcctga tcaacgtgcc gataggggcc ctgtcgctgt ttctcgtgca
     3121 ggcgctggtg aatgagccgg aggtgctcca gaaggagcgg gcgcagcgct tgaagcgggg
     3181 catccggctg gacgtgcccg gcgcggtcct gatcgcgatg ggtctcgcgt ttctcgaagt
     3241 gaccatggat cgcggcgagc gcgaggactg gtttgcgagc ccgctcatca gcatctcggc
     3301 ggtgatcgcc gggctatctc tgatttcgtt tgcggtctgg gagtggttcc agaaggagcc
     3361 gctgctggac atgaagctct tccgcaaccg caacttcgcc gtcgccaatc tggtgatcat
     3421 gatcgtcggt gtcattctgt tcggcacgac ccagttcatc ccgcagatgc ttcaggaggt
     3481 gctcggctat acggctactg ctgcgggaga ggcactgacc ctcggcggcg cagtgacttt
     3541 gatcgcgatg cccattgccg gtgtgctatc cggaaaggtg cagccgcgtg tgctgatggg
     3601 ctgtgcgctc atcgtcgagg tcctggcgct gtggaacatg acaggcttcg caacgaccat
     3661 gtccatgcac gatgccgcga tggctcgttt gtggcaggcc ctcgggatac cctttctatt
     3721 cgtgcccctg acgagtgccg cgtatgtcgg tctgcccccg caccgctcga accagggctc
     3781 ggcgatgctg aacgtggcgc gcaatctcgg tgggacgatg ggcatctccc tggtccaggc
     3841 cctgctcgcg aggcgcgagc agtttcacca gatgcgtctc gtcgagcatc tggaaccctt
     3901 gaatcccagc tacgccgcag gcctgcactc gctgacacag acgctgacga gtcaaggcct
     3961 gccgcccgcg gcggccaacc aatccgcgct cggtctcatt tatcaaacgg tgctgcagca
     4021 ggccagcatg caggcattca tcgactgttt ctggatcctg atgctgttcg tcgtcctcgt
     4081 catccccacc gtgttctttc tgaagcgaac accgctggtg cacgagtcat gagccggacc
     4141 cgccgcgcaa acccgctcgc atgcgctgtg ctcatgagca ccctggcggg atgcacggtg
     4201 ggccctgact atcggacgcc tcaggttgca gtaccggatc aatacgagcg agtgccgggc
     4261 tcttccgtcg aggcgccgct cagtcagcca ctgccgagcg aggtcgatct gtcgcagtgg
     4321 tggcagcagt ttcacgactc acaactcgat gacctgatca ctcgtgcgtt gaaggcgaat
     4381 ttggatctgc agacggctgc ttcccgcatc cgccaggcgc gcgagcagga gatcattgct
     4441 ggggcggccc gtctgccgag tgtgagtgcg acgggccttg cggcacgcct gcacacgaac
     4501 tccaacccac tggccgccct gagtggcggt cagtccggtg gcagtcagtc cggcggcggt
     4561 gcatccggac cctccggcgc cacactcgat ctctactcga tcggtttcga tgcgacctgg
     4621 gaagcggaca tcttcggcgg agtgcgccgc ggcatcgagg ctgcgcgagc caacactgag
     4681 gcggccgaat ggcagatgcg cgatgcgcag gtgtcactga gcgctgaagt cgcaaacggg
     4741 tatctcgggc tgcggaccac acagactcgc ctggcgatcc tccagcaatc cgcccagcac
     4801 cagcaggagc tgctgcagct ggcgagcgcc cgcgcccgga ccggtttcgt gacggagctg
     4861 gacgtgaatc agcagcggac gcagcttgcc gctacgatcg cgcaggtgcc cacgctccag
     4921 gccgaagagc gcgctcaggt ccatgctctc ggcgtgttgc tgggcgaggc gcctgaggca
     4981 ctgacgggtg agctggccgg ggaagccacg gcacccgcgg ttccaacaga gctgcccgtg
     5041 ggcctgcctt ccgaactgct gcgccggcgg ccggatgtcc gtcgggccga gcgtcagctg
     5101 gcggccgcga ctgcccaggt cggggtggcc gtcgccaccc tctatccgaa gttcaatctg
     5161 atcggggcgg tctcgctcgc ctcgacctcc acggggaaac tgttcgacag cagcagcctc
     5221 ggaaacttcg gcgcgggcat gatctcctgg cccatctttc aggccggcaa ggtgcgcgcc
     5281 aacgtccatg cgaacgagga gcagcagaag caggcctatc ttgcgtaccg aaagtcagtg
     5341 ctcgtcgcgt tgcaggatgc cgaggatgca ctggtgcgct acgccgccga gcagcggcgg
     5401 ctggtttcgc tgcgccaatc gctcgcggcc gccgcctcct cgcggcacat tgcggaggag
     5461 caatatcgca ccggcctggt gaccttcatc aacgtgctga gcgccacgac caccgagctg
     5521 actgcgcagg atgaggtt
//
LOCUS       DASRHN010000350         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_457515, whole genome shotgun sequence.
ACCESSION   DASRHN010000350 DASRHN010000000
VERSION     DASRHN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457515"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3331
                     /locus_tag="VFB37_14110"
     CDS             <1..>3331
                     /locus_tag="VFB37_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZO23633.1"
                     /translation="LHDGDVVLGWLPLFHDMGLMGIALHPLYKGIPSILMTPDSFIRR
                     PMSWLRAISEHGATTSGAPDFAYEMCVRLARQRDKEGLDLSRWRVAFSGSERVRPRTL
                     ARFEETFGDRGFQSRHFLPCYGMAEATLMISGRLPDERPVVRRFDARALIESQRAFEI
                     DNAVNSIELTCVGRALRDETIAIVSQDSQQKLSDGELGEVWVHSASVSEGYWESQSDS
                     RTFPSWDEARGYLRTGDVGFLQAGELFITGRLKDLIIIRGRNLHPEDVEIGVEPCLGP
                     LLTGTVVAFPTEVADQERLVVVFETEPANEHAIADLAESVKRVIGERFEVELHALCAV
                     KRGSIPRTSSGKRRRGETRSLWEEKRLSVLAQAVLADGDSPGAWRAERETLSRLPADE
                     ARALVNRDIIKWIRQASGAGTHEGDPTHEAGRAHEADKPHEALSKRSLLSLGLGSLQV
                     IRLIHEIEDHYDVSIALADAFRVVSVEALAGLIVDGIQGGATLPNRGARDADLGGATG
                     LPATTGLARDGDLAGESQLTPLSLGQQGIWFAQQVFPTSSAWQVARAAEVSPAINIDA
                     LNSALEQVVRRHAALRTAISAEHGSVPTQRVLDAVSLRAKLVSCESRAEHTELLRAEA
                     ESLIDLACPPLLRVSVLRTPGSNDVLVLVAHHIICDLWSLSLLLRDLFEFYRGVQIEL
                     VATHVPGAPCYLDHVRNEQRLASSELWRQRTQYWKGYLGAPDTVHRETALPAGERSIG
                     ELPTGQLPRGERLRGDRRRKAATHMRTVDRELSQALRRAAQQQGVSLHMLLFAAFQLA
                     VAAFEGQTEFLMCSLTSGREHRSRREVIGYFANLLPFRVQIDWCESVGPWLSRVRREL
                     LDAYANELPYEEIVRACGRREAHEAPGRYAFIMQDEGATDSAGFVPLSLGQAGGRWTL
                     AGVELCSLPFEEGDAQFPLALYAGVHDGVVRCCYKGTEATVGTEDIARVADLVDRALR
                     WLSAGQGDRILGELEWLNAAERSALVEAAATASTPAYLRPQSLLDAFEAQCRETPDRI
                     ALEFEDTSVSYAGLHRMVVNTASELQSRGVGSETIVGVCTLRSVEMVVSIYGVLKAGG
                     AYLPLEPSYPAEYLKTLVAESG"
BASE COUNT          571 a          977 c         1152 g          631 t
ORIGIN      
        1 gttacacgac ggcgacgtcg tgctcggctg gttgccgctg tttcatgaca tgggattgat
       61 ggggatcgcg ctgcacccgc tgtacaaggg gatccccagc attctgatga ctcccgacag
      121 cttcatcagg cggcccatga gctggttgcg cgcgatcagc gagcatggcg caaccaccag
      181 cggcgctccc gattttgcct acgagatgtg tgtgcggctg gcccgccagc gcgacaaaga
      241 gggtctggat ctcagccgct ggcgcgttgc attcagcggc tccgagcgcg tgcggccgcg
      301 cacgctcgcc cgattcgagg agactttcgg ggaccgcggg ttccagtcgc gccacttcct
      361 tccctgttac ggcatggctg aagcgacgtt gatgatcagt gggcgcttgc cggacgaacg
      421 gcctgtggtg cggcgcttcg atgcccgcgc cctgatagaa agccagcgcg ccttcgaaat
      481 cgacaacgcg gtcaacagca ttgaattgac ctgcgtcgga cgtgcgctgc gcgacgagac
      541 catcgcgatc gtaagtcagg actctcagca gaagctctct gacggtgagc tgggcgaggt
      601 ctgggttcac agcgccagcg tgagcgaggg ctactgggag tcgcagtcag attcgcggac
      661 tttcccttcc tgggacgagg cgcgtggtta ccttcgcacg ggggatgtgg gctttctgca
      721 agccggtgag ctgttcatca ccggccgact caaggatctg atcatcatcc gggggcgcaa
      781 tcttcatccg gaagacgtag agataggtgt ggaaccgtgc ctgggtcccc tccttaccgg
      841 cacggtcgtt gccttcccga cagaagtcgc cgaccaggaa cggcttgtcg tggtgttcga
      901 gaccgaaccg gcgaatgagc atgcgatagc agacctcgct gagtcggtga agcgggtgat
      961 tggcgagcgc ttcgaggtgg agttgcacgc gctgtgcgcc gtcaaacgcg gcagcattcc
     1021 gcgtacctcc agtggtaagc gccgcagagg ggagacgcgg agcctgtggg aggagaaacg
     1081 gctatccgtg ctggcgcagg ccgtgctggc ggacggagat tcgcctggag cgtggcgagc
     1141 tgagcgggag acgctgagcc gcctcccggc ggacgaggcg agggcgctgg ttaatcgcga
     1201 catcataaag tggatccgtc aggcgtccgg cgcgggcaca cacgagggcg atccgacaca
     1261 tgaggccggt cgagcacacg aggccgacaa gccccatgag gcactcagca agcgctcgct
     1321 gttgagtctg ggcctcgggt cattgcaggt gatccggctc attcacgaga tcgaggatca
     1381 ctacgatgtg agtatcgcgc tggccgatgc gttccgggtc gtcagcgtgg aggcgcttgc
     1441 aggcctgatc gtcgacggca tccagggtgg ggcgaccctg ccgaaccgcg gcgcgcgcga
     1501 tgccgatctc ggaggcgcca caggccttcc ggccaccacc gggcttgcgc gcgatgggga
     1561 cctcgcgggc gagtcgcagc tgacacccct gagcctgggc caacagggca tctggtttgc
     1621 gcagcaggtc tttccgacca gctctgcctg gcaggttgcg cgagctgcgg aggtatcgcc
     1681 cgctatcaat atcgatgccc tgaattcggc gctggaacag gtggtgcggc gtcatgcggc
     1741 gctacgcacc gcgatatcgg cggagcatgg cagtgtgccc acgcagaggg tcctggatgc
     1801 cgtttcgctc cgagcgaagt tggtgagttg tgagtcgcgt gctgagcaca ccgagctcct
     1861 tcgggctgag gctgagtcgc tcatcgacct ggcgtgcccg ccgctactgc gggtcagtgt
     1921 gctgcgtact ccaggctcga acgatgtgct cgtactcgtt gcgcaccaca tcatctgcga
     1981 tctgtggtcg ttgagtctgc tgctgcgcga tctgttcgaa ttctatcgtg gcgttcagat
     2041 cgaattggtc gccacgcatg tgccgggcgc accctgctat ctcgatcatg ttcgtaacga
     2101 acagcgcctg gcatcgagtg agctgtggcg tcagcgcacg cagtactgga agggctacct
     2161 gggcgcgccc gatacagtgc atcgggaaac ggcgctgccc gcaggcgaac gatctatagg
     2221 ggagctaccg acagggcagc taccgagagg ggagcgactg agaggcgacc gccgccgaaa
     2281 ggcagcgacg cacatgcgga ccgtggaccg ggagctgagc caggccttgc ggcgcgccgc
     2341 gcagcaacag ggtgtgagtc tgcacatgct gctgtttgct gcgttccagc tcgctgtggc
     2401 ggcattcgag ggccagactg agtttctgat gtgctcgttg acaagcgggc gggagcatcg
     2461 cagccggcgg gaggtcatcg ggtatttcgc caatctcctt ccgtttcgag tccagatcga
     2521 ctggtgtgag tcggtgggtc cgtggttgag tcgggtccgc agagagttac tcgacgccta
     2581 cgccaatgaa ctgccttacg aggaaatcgt ccgcgcctgc gggcggcgcg aggctcacga
     2641 ggcgcccgga agatatgcct tcatcatgca ggacgagggc gcgacggatt cagcgggatt
     2701 cgttccgctc tcgttagggc aggcaggtgg ccggtggacg ctcgccggtg ttgagttgtg
     2761 ctcactgccg tttgaagagg gcgacgccca atttcctctg gctctgtatg caggcgtgca
     2821 cgatggtgtg gtccgatgct gctacaaggg cacagaggcg acggtaggga cggaagacat
     2881 cgcgcgcgtt gccgatctgg tggatcgcgc gttgcgttgg ctttccgctg gccaaggcga
     2941 ccgcatcctc ggtgagctgg agtggctgaa tgccgccgag cggagcgcgt tggtagaagc
     3001 agcggccaca gcctccacgc cagcctacct ccggcctcag tcgcttctcg acgccttcga
     3061 agcccagtgc agagagactc ccgaccgcat cgcgctggag ttcgaggaca cctccgtcag
     3121 ctatgccgga ttgcaccgca tggttgtgaa cacggcatcc gagctgcaga gccgtggtgt
     3181 ggggtccgag acgatcgtag gcgtttgcac cctgcgttcg gtcgaaatgg tcgtatcgat
     3241 ctatggcgtg ctgaaggccg gcggcgccta tcttcctctg gaaccctcct atccggccga
     3301 gtatctcaag acgctcgtgg cagagtcggg g
//
LOCUS       DASRHN010000351         3720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_951976, whole genome shotgun sequence.
ACCESSION   DASRHN010000351 DASRHN010000000
VERSION     DASRHN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951976"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFB37_14115"
     CDS             <1..796
                     /locus_tag="VFB37_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO23634.1"
                     /translation="AQAMIDDGLFVRFPKPDVILGQHVMPLSAGIIASQSGVITSAGD
                     SFQIRMFGRGAHGSMPQASIDPVVMAASTVLRLQTIVSREVAATDAAVVTVGVLQAGT
                     KENVIPDEALIKLNVRTFDPGVREHVLAAIRRIVNAEAQASGAPRAPEIMQLDSYPLN
                     VNDKEASERVAEAFRAHFSADRVRHTGPAPASEDFGCFGTALNAPSVYWFVGGTDPAL
                     YAQAKQQGRINELPVNHSPEFAPVLHPTLETGVEAMATAAMAWVRA"
     gene            824..1480
                     /locus_tag="VFB37_14120"
     CDS             824..1480
                     /locus_tag="VFB37_14120"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HZO23635.1"
                     /translation="MSELDTGLEVSRTLVTVLDLCGTFAFAVSGAMAGVAKRLDVFGV
                     LVLAFAASSLGGIGRDVLIGATPPAALVDWRYLAVSLAAGLISFGWSALIEKLRHPVL
                     WLDAAGLAFFAVAGTQKALAFGLSPLMSALLGMLTGIGGGIVRDVLLAEIPAVLRSDL
                     YAVAALGGAAVVAIGSLVHAPVVLTAMCGGLLCFGLRVMAIRWGWHLPVADRRPAPRE
                     "
     gene            complement(1499..3289)
                     /locus_tag="VFB37_14125"
     CDS             complement(1499..3289)
                     /locus_tag="VFB37_14125"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZO23636.1"
                     /translation="MTERSVDPASDVPIQVHQPGLHELREVVARRLEEWGPHRGTLRH
                     DGVAGLSLAVANVPDGMANALLVGINPIHGLYATVIGPIVGGSLSSTQLMVITTTAAA
                     SLTASQALVGLRPEMRMTALFLMTMLVGIFLVTFVALRLQRFVRFVSYSVTTGLLAGI
                     SVLLILSQLPTVAGYAPSGGNRIAQTIDLLRHFRAIDPYSVATGALALALAMLLPRTR
                     LGSLGRLLAVAVPSALTIVLGLGSVSVVSDVGDIPRGFPTPALPEWSTLTPALISSVV
                     TGALSVAAVILVQGVGVSQTVPNPNRKRSSVSRDIVAQGIANAACGLFRGLPVGGSLS
                     GTALGVLAGARTRWTAILAGVWMAVILLAFPGLVSQVALATLGALLILAGASSIKVHE
                     ILAVWRTGWPSRLAAMSTFFAVLYLPIQAAVALGIVLSALLYLNESSTDVSLVELVEG
                     DDGQVEERAAPQRLTSDRVRVLDVYGHLFYAGARTLEGLLPNPGGTHHPVVILRLRGR
                     TSLGATMIDVLARYTEQLQKRQGRLYLTGISDEVSRQIVRTGKLELTGPVHIHEATRV
                     LGESTREALAHAQTWLVGLHSNGRVMDENG"
     gene            complement(3324..3590)
                     /locus_tag="VFB37_14130"
     CDS             complement(3324..3590)
                     /locus_tag="VFB37_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23637.1"
                     /translation="MSALLASLVEIAVVLFALSSMLSVGVGHDWRHLIGPLRNTRAVI
                     GALLANFVLVPLLMIGILPVTRQLFPWSWCLRSWGWPFSSRSRR"
BASE COUNT          706 a         1184 c         1174 g          656 t
ORIGIN      
        1 tgctcaggcg atgatcgacg acgggctctt cgtgcgtttt cccaagcccg atgtgatcct
       61 cggccagcac gtcatgccgc tgtccgcggg gatcatcgcg agccaatccg gagtaatcac
      121 atccgctggc gacagctttc agattcggat gttcggtcga ggggcacatg gatcgatgcc
      181 acaggccagc atcgatccgg tcgtgatggc cgcctcgaca gttctgcggc tgcaaactat
      241 tgtatcgaga gaggtcgccg cgaccgatgc cgccgtcgtc acggtagggg tcctgcaggc
      301 gggcacgaag gagaacgtca ttcccgatga agccctcatc aagctgaatg tgcgcacctt
      361 cgaccctggc gtgcgggagc acgtgctcgc cgcaatccgc cgtatcgtaa acgccgaagc
      421 gcaagcatcc ggtgcgccgc gggcgcccga aatcatgcaa ctcgacagtt atccactcaa
      481 tgtgaacgac aaagaggcca gcgagcgcgt ggcagaggcc ttccgcgctc acttttcggc
      541 tgatcgggtg cggcacacgg gtccggctcc cgccagtgag gatttcggat gctttgggac
      601 ggccttgaac gctccttcgg tctactggtt cgtgggcggc acggatccgg cgctgtatgc
      661 gcaggcgaaa caacagggcc gcatcaatga attgccggtg aatcacagtc cggaattcgc
      721 gcccgtgctg catccgacac tggaaaccgg agtcgaggcg atggctaccg ccgcgatggc
      781 gtgggtgaga gcctgatgag agcgcctgcg cgcctgctca aacatgagtg agctggacac
      841 cggcctcgag gtgtcgcgca cgctggtcac cgtccttgat ctttgtggga cgtttgcttt
      901 tgcagtcagt ggcgccatgg cgggagtggc gaagcggctg gatgtattcg gggtgctggt
      961 cctggccttt gcggcgtcca gtttgggtgg gatcgggcgt gatgtgctga tcggtgcgac
     1021 gcctccggcg gcgctggtgg actggcggta tctggccgtg tcgctggcgg ccggtttgat
     1081 ctctttcggc tggtccgccc tgatcgaaaa gctcaggcac ccggtgctgt ggctggatgc
     1141 agccggcctg gcgttcttcg ccgtggccgg cacccagaag gcgctcgcct tcggactgag
     1201 cccactgatg tccgcactgc tgggcatgtt gaccggaatc ggcggcggca tcgtgcgcga
     1261 cgtcctgctc gcggaaattc cggcggtgct gcgttcagat ctctatgccg tagcggcctt
     1321 ggggggcgca gccgtcgtgg ccatcggcag tctcgtgcac gctccggtcg tactcactgc
     1381 gatgtgcggc gggctgctct gctttggtct gagagtgatg gcgatccgat ggggatggca
     1441 cctgccggtc gccgatcgaa gacccgcacc gcgagaatag gtcgcgagca gttccttcct
     1501 acccattctc atccatcacg cgcccatttg aatgcagacc caccaaccag gtctgcgcat
     1561 gcgcaagcgc ttctcgcgtc gattccccga gcacgcgagt ggcttcgtga atatgcactg
     1621 ggccggttag ttcgagctta cccgtgcgta cgatctgacg agaaacctcg tcgctgatgc
     1681 ccgtgagata gagccgtccc tgcctcttct gcagctgctc ggtatatcga gccagcacat
     1741 cgatcatcgt cgctccgagt gaggttcggc cgcgcagacg cagtatcacg acaggatggt
     1801 gagttccccc cgggttgggc agcaatccct cgagcgttcg ggcgcctgca tagaagagat
     1861 ggccatatac gtcgagcacc cgcacccgat ccgatgtcag cctctgcggc gcggcgcgct
     1921 cctccacctg cccgtcatcc ccctccacga gctccaccag cgagacgtcg gtcgaagact
     1981 cgttcagata cagcaatgcg gagagcacaa ttccgagagc gactgcggcc tggatcggca
     2041 gatacagaac cgcgaaaaac gtgctcatcg cggccagccg tgagggccag cccgttctcc
     2101 agaccgccag aatctcgtgg accttgatgc tactggcgcc agcgaggatc agtagcgcac
     2161 ccagggtggc cagcgcgacc tgcgaaacaa gcccgggaaa ggcgaggagg atcacggcca
     2221 tccacactcc agccagtatt gccgtccaac gtgttctagc gccggcaagc actcccagcg
     2281 ccgtgccgct cagtgagccg cccaccggca ggccacgaaa cagcccgcag gcagcgttcg
     2341 caattccctg tgccacgata tcgcgagaca cactcgagcg ctttcggttg ggattgggaa
     2401 ccgtctggct gaccccaact ccctgcacca gaatgaccgc cgcgacggac agggcgcccg
     2461 tcacgaccga gctgatcagc gccggtgtaa gagtggacca ttccggcagc gccggagttg
     2521 gaaagccccg cgggatgtcc cccacgtcac tgaccaccga cacgctgccc agtccgagga
     2581 caatggtgag agcagacggt accgcgacgg ccagcaaacg tccaagcgat cccagccgtg
     2641 tgcgagggag cagcatggca agcgccaggg caagcgctcc cgtggcgacc gagtacgggt
     2701 cgatggctcg aaagtgcctc aacaggtcaa tcgtctgagc gattcggttg ccgccactcg
     2761 gcgcataacc ggccaccgtg ggcagctgac tgagaatgag caatacggaa ataccggcca
     2821 gcagaccggt ggtgaccgag taggagacga agcggacgaa gcgctgcagc cgcagggcga
     2881 cgaaggtcac caggaaaatg cccaccagca tcgtcatcag gaatagcgct gtcattcgca
     2941 tctcgggccg cagtccgaca agcgcctggc tcgctgttag agaggccgcc gccgtcgtcg
     3001 tgatgaccat gagctgtgtg ctggacagtg agccgcccac gatcggcccg atcacggttg
     3061 catagagccc gtggatcgga ttgattccga ccaggagcgc gtttgccatt ccgtccggca
     3121 cgttggcaac ggcgagactc aatccagcga ccccgtcatg acgcagcgtg ccgcgatgcg
     3181 gtccccactc ctcgaggcgc ctcgccacca cctcgcgcag ctcgtgcagt cccggctgat
     3241 gaacctgaat cggtacgtcg gacgcagggt cgacagagcg ttccgtcact gcgagcccgc
     3301 ctctttgatc cggcgtttgc gcatcaacgc cgcgatcggg aagagaacgg ccaaccccag
     3361 gagcgaagac accacgacca tggaaacagt tgtcgggtca ccggcagaat gccgatcatg
     3421 agcagcggca ccagcacgaa gttggcgagg agggcgccga tgaccgcgcg agtgttgcgc
     3481 agcggtccga tgagatgacg ccagtcgtgg cccactccca cggacagcat gctgctcaac
     3541 gcgaagagca cgaccgcgat ctccaccaga ctcgccagca atgcactcat ggatcgtcac
     3601 gcaagcagct cttggggaac gcttccaacg gtcgcactga tgcgcctgcg ctcgcgcctc
     3661 attccttggc gagtgccaat tcgagtcttt cctcctgcgc aatttcgcca tcgaaatcag
//
LOCUS       DASRHN010000352         5119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_458029, whole genome shotgun sequence.
ACCESSION   DASRHN010000352 DASRHN010000000
VERSION     DASRHN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458029"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="trkA"
                     /locus_tag="VFB37_14135"
     CDS             <1..905
                     /gene="trkA"
                     /locus_tag="VFB37_14135"
                     /inference="COORDINATES: protein motif:HMM:NF007030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HZO23638.1"
                     /translation="VRALKGGLLVGHALRELPQHLRKADARVVAIYRAGRSMATEGDT
                     LIEDGDEVFFLAARSDIRRVMNEMRKQDDPVRRIVIAGGGNIGLRLARTLETSNQVKL
                     IERDAKRARKASELLDRAIVLNGDAADEELLIEENIDSADVFAALTNSEEANILSAML
                     AKRLGAHKVMALVNRPSYGELIESGSIDIAISPRTITLGSLLAYVRRGDVVRVHSLRR
                     GAAEALEAVVHGEQANSRVVGRRVEEIELPEGTTIGAIVRGDEVIMAHHDTVLQSDDH
                     AILFLSDRRHLEAVERFFVRTSPQ"
     gene            1063..2511
                     /locus_tag="VFB37_14140"
     CDS             1063..2511
                     /locus_tag="VFB37_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZO23639.1"
                     /translation="MLAVVHVLGQMLAFFGAAYILPIACSLLTSDGLALYFLAGAGAT
                     TGLGLALVGATRKSRRELKPRDGFLLVTAGWILTSALATIPLLLAMPRLGFTRAFFET
                     MSGLTTTGSTVLSGLDHLAPSLNLWRHALQWVGGLAIIVMALAVLPVLGIGGMQLYKS
                     QTPGQIKDDRLAPRIAQTARSLWLVYTTLTVAGIIALRICGMSWLDAICHAFSAVGLG
                     GFSTHDANVAWFNSTAIELVLMALMLIASLNFARHIVAFRRLSTQPYTRDPESRAIVL
                     VLAASIVLITALLSWHGRYPNVLVSLRHAAFNVISMATTSGLSTEDLDTWPVFAPFWL
                     LFLSCIVCSTGSPGGGIKMFRALVLAKQAHHELKMLVHPSAVVPVRIGGRPIPERVAD
                     AVLGFIFLYFMTVALLTFALLLTGLDFDSSFSAIVAAINNTAHGLHTKGPVSNYHALT
                     AAQTWICTAAMLLGRLEIFSVIVLFTPAFWRK"
     gene            complement(2531..2959)
                     /locus_tag="VFB37_14145"
     CDS             complement(2531..2959)
                     /locus_tag="VFB37_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZO23640.1"
                     /translation="MTTVRQLLERKGRAVFSVKPDDPVLEAVRMMADRHVGALLVMSA
                     DELQGIVSERDYARKVILHGRSSADTPVAQIMSSPVVTVSLGQTVQDCMQLMTERRLR
                     HLPVVEAGKVIGMISIGDLLKALLEEQQQTIQQLETYIHS"
     gene            3242..3694
                     /locus_tag="VFB37_14150"
     CDS             3242..3694
                     /locus_tag="VFB37_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23641.1"
                     /translation="MSPSREITVAPDRFEVTGEDAVLVTELQSAIAVCVYDAVEEAGA
                     LLHLRCLVRSPKPVDMTDTTLATELLLLDRCVETMRDLSPGARNLQARVVAHLADHPR
                     ARETCDSMLTMVQHFLADAGIRELPVDVAGGQARAVRFRPSMGWLQTR"
     gene            3764..>5119
                     /locus_tag="VFB37_14155"
     CDS             3764..>5119
                     /locus_tag="VFB37_14155"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZO23642.1"
                     /translation="MSRLVCVSNRISLPRRGATPGGLAVGVLAALQHTGGVWFGWSGQ
                     TPDIPAENPEILTRGNIRFATIDLKPAEFDAYYNGFCNGTLWPLFHYFPDRFTHEQRQ
                     SEAYQSVNERFAQQLMKVLKPGDAIWVHDYQLISLGRYLRQLQFTGRIGFFLHIPFPH
                     MQILRLLPNYQALVRDMCQYDLLGFQTEDDVRSFLTCLEPERTTLPADRRIEIGGRGV
                     MLGAYPIGIDVEEVSREAVRTISQETVQRMIVSLMGRKLILGVDRLDYTKGLTERFAA
                     FEHFLEAFPDNQGNVTFLQIAPLSRSDVLAYSEIRQALEQRAGRLNGRFAEADWTPIR
                     YLNRDYARSTLMGVLRASQVALVTPVRDGMNLVAKEYVAAQDPKDPGVLILSPMAGAA
                     RELTSALQVNPYDKRGMALALQEALTMPLEERRQRHEQMLEAVRHQDIHNWYGRFVYD
                     LT"
BASE COUNT          860 a         1701 c         1597 g          961 t
ORIGIN      
        1 gggttcgtgc gctcaaggga ggcctgctgg tcgggcatgc gctgcgcgag cttccgcagc
       61 atctgcgcaa ggccgatgct cgggtcgtcg cgatataccg ggccggacgc agcatggcca
      121 ccgagggcga caccctcatc gaggacggcg atgaggtttt ctttctcgcc gcccgcagcg
      181 acatccgccg cgtgatgaac gagatgcgta agcaggatga ccccgtgcga cgcatcgtga
      241 tcgccggagg ggggaacatc ggccttcggc tcgcgcgtac gctggagacg agcaatcagg
      301 tgaagctgat cgagcgggat gccaagcgcg cccgcaaggc ctccgagctg ctcgaccggg
      361 cgatcgtgct caatggcgat gcggcggatg aggagctgtt gatcgaggaa aacatcgaca
      421 gcgccgacgt gtttgcggcg ctcaccaact cggaagaggc caacatcctg tcggccatgc
      481 tggcgaaacg cctgggcgcc cacaaggtca tggccctggt caataggccg tcgtacggtg
      541 agctgattga aagcggcagc atcgacattg cgatctcgcc gcggaccatc acgctcggct
      601 cgctgcttgc atacgtgcgc cgcggcgatg tcgtgcgcgt gcactcgctc aggcgtggcg
      661 cggcggaggc tctcgaggcc gtcgtgcatg gcgagcaggc caactcaagg gtcgtgggca
      721 ggcgggtcga ggagatcgag cttcccgaag gcacgacgat cggtgccatc gtgcgcggcg
      781 atgaggtgat catggcgcat cacgatacgg tccttcagtc ggacgaccat gccatcctgt
      841 tcctctccga tcgccgccac ctggaagccg tcgagcggtt cttcgtgaga acctcgccgc
      901 aatgagctgg cgctcgccac gcggccgggc ggtgtggccc gcatataaga gaccgctcac
      961 cgaggcgcag ccggcaaaag tcacgccttt tgccggcggc aacggcacca atgtccgaaa
     1021 aacggccgca ggcccgtttt tcccatagac aagggcagcc gggtgcttgc agtcgtacac
     1081 gtcctcgggc agatgctcgc gttcttcggc gctgcctaca tcctcccgat cgcgtgctcg
     1141 ctgctcacct cagacggact cgcgctgtac ttcctggccg gtgccggcgc gaccaccggt
     1201 ctcggtctgg ccctggtcgg cgcgacccgc aagtcgcgcc gcgagctcaa gccgcgcgac
     1261 ggattcctgc tggtcaccgc cggctggatc ctgacttccg ctctcgcgac catcccgctg
     1321 ctgctcgcaa tgcctcgcct gggcttcacg cgcgcgttct tcgagacgat gtccggtctc
     1381 accacgacgg gctcgaccgt gctcagcggc ctggatcatt tggccccgtc gctgaacctg
     1441 tggcggcatg cgctgcagtg ggtcggcggc ctcgccatca tcgtcatggc cctggccgtg
     1501 ctgcccgtgc tgggcatcgg tggcatgcag ctctacaaga gccagacgcc gggtcagatc
     1561 aaggacgacc gcttggcgcc gcgcatcgct cagaccgcgc gctcactctg gctcgtgtac
     1621 acgacgctca cggtcgcggg aataatcgca ctgcgtatct gcggcatgtc ctggctggac
     1681 gccatttgcc atgccttctc ggccgtcgga ctcggcggct tctcgacgca cgacgccaat
     1741 gtcgcctggt tcaactccac agcgatcgag ctggtgctga tggcgctcat gctcatcgcc
     1801 tcgttgaatt tcgcgcgcca catcgtcgcg ttccgtcgcc tctcgaccca gccctatacc
     1861 cgcgatcccg agtcgcgcgc catagtcctt gtcctcgccg cgagcatcgt gctgatcacc
     1921 gcgctgctga gctggcacgg tcgctacccg aacgtactcg tctcactgcg ccacgcagcc
     1981 ttcaatgtca tctcgatggc gaccacgagc ggactgtcca ccgaggacct ggatacctgg
     2041 cccgtgttcg cccctttctg gctgctgttt ctatcctgca tcgtctgcag caccggctcg
     2101 ccgggcggcg gcatcaagat gttccgtgcc ctggtgctcg cgaaacaggc ccatcacgag
     2161 ctcaagatgc tggtgcatcc gagcgccgtg gtgccggtgc ggatcggcgg gcgccccatc
     2221 cccgagcgtg tcgccgacgc ggtcctgggc ttcatcttcc tgtatttcat gacggtcgcg
     2281 ctgctgacct tcgcgctgct gctcaccggt ctcgacttcg attcgtcgtt cagcgcgatc
     2341 gtcgccgcaa tcaacaacac ggcccacggc ctgcacacca agggaccggt cagcaactac
     2401 cacgccctga cggcggcgca aacgtggatc tgcacggctg ccatgctcct ggggcgcctg
     2461 gagatcttca gcgtcattgt gttgttcaca ccggccttct ggcggaaata gccggggcgc
     2521 gtgggcggct ctagctgtgg atataggtct cgagctgctg gatcgtctgc tgctgctctt
     2581 cgaggagcgc tttgagcagg tcgccgatcg atatcatgcc gatcaccttc cccgcctcga
     2641 cgaccggcaa atggcgcaaa cgtcgctccg tcatgagttg catacagtcc tgcaccgtct
     2701 gcccgagtga caccgtcacc acgggcgagc tcatgatctg cgcgaccgga gtgtcggccg
     2761 atgagcggcc atgcaggatg actttccgcg catagtcgcg ttcggaaacg attccctgca
     2821 gctcgtccgc actcatgacg agtagagctc cgacgtggcg atccgccatc atgcgtaccg
     2881 cctccagcac cggatcgtcg ggcttcaccg agaacactgc tcggcccttg cgctcgagaa
     2941 gctgtcgtac ggttgtcatg tcaggtctcc gtgaaagcgc gcccggacgc gacggcgaga
     3001 ccccgctgtc ttagcgcgta acccccgtca ctgtcaacgc tctgcgagga gctgtcgcaa
     3061 ccagcgaaca cggctgaatc ggaaccggca cagtgacgca atccacactg atccggacgt
     3121 gtgaaatgac gcacgagacg aagttaccaa tgcacgtatt gcagccggtc cagcgtgcgg
     3181 cagttcaaag tttgagcagc cgggatgacg gcggagccgg cgtgccggta taccaactcc
     3241 tatgtcaccg agccgcgaga tcaccgttgc gcccgaccgg ttcgaagtca ccggcgagga
     3301 cgcagttctc gttaccgagc tgcaatcggc gatcgccgtt tgtgtgtacg acgccgtcga
     3361 ggaagccggg gcgctgctgc atcttcgctg cctggtgcgc tctccgaaac ccgtggacat
     3421 gacggacacg acgctggcca ccgagcttct gttgctcgat cgatgcgtcg agaccatgcg
     3481 cgatctttcc ccgggtgccc ggaatctgca ggcgcgggtg gtcgcacatc ttgccgacca
     3541 tccccgtgca cgcgagacct gcgattcgat gctcacgatg gtgcagcact tcctggccga
     3601 tgcaggcatt cgagagctgc ccgtagatgt agccggcggg caggcgcgcg cagtacgatt
     3661 ccgccccagc atgggttggc tgcagacgcg ctgaagctct ttccggcgcc cctccggccg
     3721 gcccaccccg gaacagtttg cctcggcagg cgttagtggg cacgtgagcc gcctagtttg
     3781 cgtttcgaac cgtatttcgc tgccccgccg gggcgcgact ccgggcggtc tagctgtcgg
     3841 agtgctcgcc gcgctccagc acaccggtgg cgtgtggttc ggctggagcg gtcagacccc
     3901 ggacattccc gccgagaacc ccgagatact cacgcgaggc aatatccgct tcgcgaccat
     3961 tgatctgaag cccgctgagt tcgacgctta ttacaacggg ttctgcaatg gaacgctgtg
     4021 gccgttgttc cactacttcc cggaccggtt cacccacgag cagcggcagt ccgaggctta
     4081 ccagtccgtc aacgagcgct tcgcgcagca gctcatgaag gtgctcaagc ccggcgatgc
     4141 gatctgggtg cacgactatc agctgatctc actcggacgc tatctgcgtc agctgcagtt
     4201 cacgggccgc atcggcttct tcctgcacat tccttttccg cacatgcaga tcctgcggct
     4261 actcccgaat tatcaggcgc tggttcgcga catgtgccag tacgatctgc tgggcttcca
     4321 gaccgaggac gacgtgcgct ccttcctcac gtgcctcgag cccgagcgca ctaccttacc
     4381 tgcggatcgg cgaatcgaga tcggcggacg cggagtcatg ctgggcgcct acccgatcgg
     4441 tatcgatgtg gaggaagtca gccgcgaggc ggtccgcacg atctcgcagg aaacggtgca
     4501 gcgcatgatc gtgagcctga tgggcaggaa gctcatcctg ggcgtcgacc gtctcgacta
     4561 cacgaaaggc ctgaccgagc gctttgctgc gttcgagcat ttcctcgagg cgttcccgga
     4621 caaccaaggc aatgtgacct tcctgcagat cgcgccgctc agccgctcgg atgtgctcgc
     4681 ctacagcgag atccgtcagg cgctcgagca gcgcgcggga cggctcaacg gccgcttcgc
     4741 cgaagcggac tggaccccga tccgctatct gaatcgcgac tacgcgcgct cgacactcat
     4801 gggcgtcctg cgcgcatccc aggtcgcgct cgtgacaccg gtacgcgacg gcatgaatct
     4861 cgtggccaag gagtacgtgg ctgcgcagga tccgaaggat ccgggcgtgt tgattctgtc
     4921 gccgatggca ggggccgccc gtgagctcac gagtgccctg caggtgaatc cttacgacaa
     4981 gcgtggcatg gcgctggccc tgcaggaggc attgaccatg ccgctcgagg agcggcgcca
     5041 gcgtcacgag cagatgctcg aggccgttcg ccaccaggac atccataact ggtatgggcg
     5101 cttcgtctac gatctcacg
//
LOCUS       DASRHN010000353         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_292449, whole genome shotgun sequence.
ACCESSION   DASRHN010000353 DASRHN010000000
VERSION     DASRHN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292449"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1313)
                     /locus_tag="VFB37_14160"
     CDS             complement(39..1313)
                     /locus_tag="VFB37_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZO23643.1"
                     /translation="MSERDRSQQSGAQAGDFKPRLMKLSILTAALQELTPRERREADP
                     DLAVEEWLEFASQLGCPNIELSAALHPSESDVPAAAMLDPVANTLDLRRPFNTERAAR
                     VRRAMQATGVGLSDLAYFDNMLAPDEAVRNKKYALMLRVFDAAVLLGAGAVTGFVGRN
                     PLLSMDANLVMFEEVFIPLLREAKARGLTFRVEQCPMPGWTSTDTWHNNIAYAPGPWI
                     ALHRICERHAVGDQFRIHYDPSHAILMGQDSRSVFQYLKDTGYHFLIGGFHVKGQVID
                     ARGISAWGYGGQTLQRGDWNGAQLSADPAARQHAWKKQTVFCEHELPGTARHDPIAYL
                     QNRSVDWLDHQLAARELLALEPDKTFLVVEHEFAPARIQDRSRLAPILAGSLAFTRAI
                     DEAAAAMYALQHEVLAAQGIPAQGVGREAYRS"
     gene            complement(1346..>2131)
                     /locus_tag="VFB37_14165"
     CDS             complement(1346..>2131)
                     /locus_tag="VFB37_14165"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO23644.1"
                     /translation="YLFGWDSPVQMAHGALARTFLLCSIASFVLGAYSFTLPRTPPTA
                     RDARGLGRLFGVEALALLRQPNFAVFFVASVLICIPLAFYYQNANQFLTEIHMPHATG
                     KQTLGQMSEVGFMLALPLFLNRFGMKLTLLLGMLAWAARYVLFAFGDAQGLGLMLLVG
                     IALHGVCYDFFFVSGQIYTDSKAGVQHKAAAQGLITLATYGVGMLIGFWAAGMIDDRY
                     ATAGAHDWRSIWLYPAIFAAGVFVLFASTFRNERIAYSAARSS"
BASE COUNT          415 a          674 c          705 g          337 t
ORIGIN      
        1 gctcacgaac ggcatgtcag cccggtactc cagttgagtc aggatcgata cgcttcacgg
       61 cccactccct gcgccgggat tccctgcgcg gcgaggacct cgtgctgcag ggcatacatt
      121 gcagccgccg cctcatcgat cgcgcgcgta aaggccagag agcctgcaag gatcggcgcc
      181 aggcgtgacc tgtcctggat gcgggccggc gcaaactcgt gctcgacgac gagaaacgtc
      241 ttatcgggct cgagcgcgag cagctcccgg gcggcgagct gatggtccag ccagtccacc
      301 gagcggttct gcaggtatgc gatgggatca tgccgcgcgg tccctgggag ctcgtgctcg
      361 cagaacaccg tctgcttctt ccaggcatgc tggcgtgccg cgggatcggc gctgagctgc
      421 gccccattcc agtcaccgcg ctgcagggtc tggcccccgt atccccaggc agagatgcca
      481 cgcgcgtcga tcacctggcc cttgacgtga aagccgccga tcaggaagtg gtagccggtg
      541 tccttcagat actgaaagac cgagcgcgaa tcctgtccca tgagaatggc atgcgagggg
      601 tcgtaatgaa ttcggaactg atcgcccacc gcgtgccgct cgcagatccg gtgcaaggcg
      661 atccagggac cgggggcata ggcgatattg ttgtgccagg tgtcggtgct ggtccacccg
      721 ggcatcgggc actgctccac tcgaaaggtc aggccgcgcg ccttggcctc gcgcagcagc
      781 ggaatgaaga cctcctcgaa catcacgaga ttggcgtcca tgctcagcaa cggattgcgc
      841 ccgacgaagc ctgtcacggc accggcaccg agcagcaccg cagcatcgaa cacgcgcagc
      901 atcagggcgt actttttgtt acgcaccgct tcatcgggcg ccagcatgtt gtcgaaatag
      961 gctagatccg aaagccccac gccagtggcc tgcattgcgc ggcggacgcg tgcggcacgc
     1021 tcggtattga acggacggcg caagtccaag gtgttggcga cgggatcgag catcgctgcc
     1081 gccggcacat cgctctcgga aggatgaagc gcagcggaaa gctcgatgtt cgggcacccg
     1141 agctggcttg cgaactccag ccactcctcg acggcgagat ccgggtcggc ttcccgccgc
     1201 tcgcgcggcg tgagctcctg cagggcggcc gtgaggatac tgagcttcat gagccggggc
     1261 ttgaagtctc cggcctgtgc tccggactgc tgtgaacggt cgcgctcact caaggtggca
     1321 tctccgtgga atccgcggcg ggccgtcaac tcgaccgtgc ggccgaatac gcgatccgct
     1381 cattgcggaa ggtcgatgcg aacagcacga aaaccccggc cgcaaagatg gcagggtaca
     1441 gccagatcga gcgccagtcg tgggcgccgg ccgtggcata gcggtcatcg atcatgcccg
     1501 cagcccagaa accgatcagc attccgacgc cataggtggc gagtgtgatc agcccctgcg
     1561 cggcggcctt gtgctgcacg ccggccttgg agtcggtata gatctgtccg gagacgaaga
     1621 agaagtcgta acagacccca tgcagcgcga tgcccacgag gagcatgagg cccaggccct
     1681 gggcgtcacc gaaggcaaag agcacgtagc gagcggccca cgcgagcatg cccaggagca
     1741 gcgtcagctt catgccgaag cggttcagga acagcggcag cgcgagcatg aagcccactt
     1801 cgctcatctg cccaagcgtc tgcttgccgg tcgcgtgcgg catgtggatt tccgtcagaa
     1861 attgattcgc gttctgataa tagaaggcga gcggaatgca gatgaggacg gaggccacga
     1921 agaacacggc gaaattcggt tgccgcagca acgcgagtgc ctcgaccccg aacaggcgtc
     1981 cgaggccgcg cgcgtcgcgg gccgtcggcg gcgtgcgcgg cagcgtgaag ctgtaagcgc
     2041 cgagcacgaa cgaggcgatc gagcacaaca ggaacgtgcg cgcgagcgcc ccgtgggcca
     2101 tctgtaccgg cgagtcccat ccgaacaggt a
//
LOCUS       DASRHN010000354        21308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2430914, whole genome shotgun sequence.
ACCESSION   DASRHN010000354 DASRHN010000000
VERSION     DASRHN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430914"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFB37_14170"
     CDS             <1..475
                     /locus_tag="VFB37_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23645.1"
                     /translation="NKALAHGQLAAIDNQVDVTTGTVKLKAEFANVDDALFPSEFVNA
                     RLKIETLTGQTLIPSAAVQQGAPGAYVYVANSNNTVTLRKVTTGPAAGELVSITRGVN
                     PGERVVIDGLDKLRDGGKISVVAAAPGTPATPGTRPASPASKAPGTTARKSAPTA"
     gene            484..3621
                     /locus_tag="VFB37_14175"
     CDS             484..3621
                     /locus_tag="VFB37_14175"
                     /inference="COORDINATES: protein motif:HMM:NF007798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HZO23646.1"
                     /translation="MNPSAIFIERPVATWLLMVSILLAGMVGYHVLSVSALPEVDYPT
                     IQVQTLYPGASPDVTTSSITAPLERQFGQMPGLSQMTSSSSGGASVITLQFSLDLSLD
                     TAEQEVQAAINAASSFLPVDLPMPPVYSKVNPADAPVLTLAVTSPTLPLTQVEDLVDT
                     RLVPKLSEVPGVGLVSLGGGQRPAVRIRVDPTLLASHNLMLEDVRTAIGNANVNQAKG
                     SFDGPLRAYTIDANDQLRSADDYRNIVLAYNNGAPLRLGDVATVVEQAENVKLAAWSA
                     SAKDGKLTPAIILNIQRQPGANVIQTVDRIKALLPQLQTSLPGSVDVAMLTDRTISIR
                     ASVRDVQFELMLAVALVVMVIFLFLRSASATVIPSIAVPLSLVGTFGVMYLAGFSLNN
                     LTLMALTIATGFVVDDAIVMIENISRHVEEGMPRRQAAFEGSRQIGFTIISLTVSLIA
                     VLIPLLFMADVVGRLFRQFAVTLAVAILISAFVSLTFTPMMCARILVPPDEKKEGRIL
                     RTFGRGFESLSHHYGRALTWVLDHERLTLIIAVATLVLTALLYVLIPKGFFPPQDTGV
                     IQAITQAPPAVSFSQMSQLQSQLARVILQDPAVDSISSYIGVDGTNTTLNSGRMLINL
                     VAKSDRNVDASGLIRRLQPKVANIQGITLYMQPVQDLTIDTTVSRTQYQLSAEDPNST
                     ELSTWIGRLVGKLQTEPTLADVASDLNDQGLEVYVDIDRDAAGRLGVTAAAIDNALYD
                     AYGQRLVSTIFTQSNLYRVVLEVRPRDQQGPEKIQDLYVATVNGNRILLSSVAQISQR
                     ISPLVVNHLGQFPAATISFNLAPGASLGEAVKIIRNDEKAIGLPDSVQTSFQGAAQAF
                     QASLSNTLWLILAAIITVYIVLGVLYESYIHPVTILSTLPSAGVGALLALMVAGQDLG
                     IVGIIGIVLLIGIVKKNAIMMIDFALQAERGEGKAPREAIYQAALLRLRPILMTTMAA
                     LLGALPLMLGTGMGSELRHPLGITMVGGLLVSQVLTLFTTPVIYLGFEQLAQWRRRLK
                     KKPDDDASQAPVTVT"
     gene            3638..6745
                     /locus_tag="VFB37_14180"
     CDS             3638..6745
                     /locus_tag="VFB37_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZO23647.1"
                     /translation="MNLSRPFILRPVATTLLTLGVIIAGVVAFRLLPVSPLPQVDFPT
                     ISVSASLPGASPDTMAATVAAPLERSLGRIASVTEMTSASALGSTRITLQFDLSRDID
                     GAARDVQAALNAARALLPTGLPSNPTYRKVNPADAPIMIIALTSQALSRGQMYDAAST
                     VLAQKLLQVNGIGDVTVGGSSLPAVRVELNPARLNGYGLSPETVRNAIVATNPNRPKG
                     SIDDGERRWQILANDQATTAKDYMPLIVAYNNGNAVHLSDIAEVVDSVQDVRNDGISN
                     GEPAVLLVLFRQPGANIIETVDRVNALLPHLRASIPASVALTVVMDRTPTIRASLREV
                     ERALMISVALVVLSVFLFLRSGRAALVPLVAVPVSLIGTFGVMYLAGYSLDNLSLMAL
                     TIATGFVVDDAVVVLENISRHIEEGMQPLQAALQGAREVGFTVLSMSISLIAVFIPIL
                     LMGGIVGRLFREFAVTLSTAILVSLVVSLTATPMMCARILRPRDEEPEHGRFYHASER
                     AFDALQSGYHRSLDWALERSPLMMLILGITIALTIYLYIAIPKGFFPQQDTGRLSGSI
                     QADQSISFQAMQQKLRDFVAIVRKDPAVTGVVGFTGGGQRNSGNMFIALKPLADRKLS
                     ADQVIARLRGKLSHEAGANLFLQSVQDIRVGGRSSGAQYQYTIQADTTDELRSWEPKI
                     RTALSSIKDLADVNTDAQDKGLQTSLVVDRDAVARLGLNQNLIDATLNDLFGQREVST
                     IYNPLNQYHVVMEAAPAFWQSPTILSDVYFTAPNNKQVPLGDFSTWGTTSTPLVVNHQ
                     GQSAASTISFNLAPGVSLSNATKDIDSAFVRLRAPATIRGTFQGTAKAFQDALANQPL
                     LILAALVAVYMVLGILYESLVHPVTILSTLPSAGVGALLALLLTGTEFSIIALIGVIL
                     LIGIVKKNAIMMIDFALQLERDQQLEPREAIHRASLMRLRPILMTTVAAMFGAMPLAV
                     GTGDGAEMRRPLGIAIVGGLLLSQLLTLYTTPVIYVYFDRFSTWAKRRLHRSPQPAAP
                     AHGVA"
     gene            6742..8295
                     /locus_tag="VFB37_14185"
     CDS             6742..8295
                     /locus_tag="VFB37_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO23648.1"
                     /translation="MSRLSTMCRLTAAQLVPTRTLAAMLVLGCTLAACSVGPDYKRPK
                     VDTPPAFKEAEGWKVAEPNDTAPKGNWWDVYNDPALDNLVKQVQVSNQNVMVAEAQYR
                     QALALVSQARANYFPLITADASSLRSQNALVTGVSGTGTSTGTPTTGNPGTGTGTGGG
                     SQNQTIPGATGTRNVDRVSLSLSWEIDVWGRIRRQVESNKAAAVASESDLANALLSAQ
                     AMLVQSYVQLRATDTDIDLYEKSVAAYDKALKITMNRYEAGVAQRTDVTQAQGQLESA
                     KAAGFDLQVTRATLEHAIAVLVGKPPAELSIERTNQLPNIPETPVSVPAALLERRPDI
                     AGAERRAAAANALIGVARAAYFPELSLTGSGGYESNTFNHLFSVPNRFWSLGPELAQT
                     VFDAGAIHAQVKQNIAAYDASVATYRQTVLAAFQDVEDNLATLKWLSQEAVAQAAAAA
                     AAEQTLSITENQYQAGTVDYLNVVIAQQSALTAERAVRDLANRRLTASVGLLKALGGG
                     WQTTMLTHE"
     gene            8498..9304
                     /locus_tag="VFB37_14190"
     CDS             8498..9304
                     /locus_tag="VFB37_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23649.1"
                     /translation="MRSPALSAVLTLLLITLSWTLPAEAAHRAHKTAEGKADARAISA
                     AIASPDRPQADHERDAWAKPELVLTFLGARPGMHVIDYLAADGYYSELLARVAGPKGQ
                     VIVYNNGGYSSFIGQKLAKRFADHRLPNTLVKVTEIADLKLPANSLDAALFVMSYHDV
                     YYTPKGASGPMGDAGQMVSALFTALKPGGVVVVQDHAAQAGTSPAESVQKLHRIDPEA
                     VKKTFEQAGFKLDAQDDAFKNSEDDHSKLVFDPSIRHKTDEFLFRFRKPA"
     gene            9316..9837
                     /locus_tag="VFB37_14195"
     CDS             9316..9837
                     /locus_tag="VFB37_14195"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZO23650.1"
                     /translation="MSFSLYEATIPSYRQVLGAIGGLLVKAEAFCSEKSLPPQELIEA
                     RLAPDMYPFAYQVKSTAVHSLGAIEGVRKGVFSPDVTTPPPTFAALKARIEETITALE
                     AVTPGEMEGFIGRDMAFVHGDFRIEFTAENFLLSFSQANFYFHAATTYDILRWKGVPI
                     GKRDFMGRVRKKK"
     gene            complement(9887..11974)
                     /locus_tag="VFB37_14200"
     CDS             complement(9887..11974)
                     /locus_tag="VFB37_14200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO23651.1"
                     /translation="MTTHVRLRVASTLAALVLAYAALPLHADESSGSGQFTVGKYLDL
                     QTASFPRLSPDGTQVVYTRSVVNKLLDKHDMSIWIVDAEGQHDRFLAEGSDAVWSPDS
                     KSIAYLGEGRPGGEGKPKGKQIFVLHLNVPGPASQITTLDHDPANLHWSPDGRRIGFT
                     MVVPSPEKWKIDLPEAPEGAKWAAAPRYTERVHYRRDGVGLTDRGYRHLFLVDADGGA
                     AREITTGDWDIGDTVHEFSDAVDWSFTPDGRSAIVEGFKEGDEDRNDQDCYIYSVDLD
                     TGTTKRLITTTGGWRMPTLSPDGKTIAYVGFTKNGDSYRVADLWTMSADGSNETLRSG
                     GFDREPQHLEWAPDSSAIYFTAEDHGNVHLYSWSSHGGVHALTSGSEVVQNASAGRNG
                     VTFVRSDFHSPGDVELINPGRPNAARKLTHLNEELLHGMALASEEEIGVDSSGGAHIQ
                     GWIVKPAGFDTSKKYPLLLEIHGGPHGMYSVAFNPSFQNFAANGYVVLYINPRGSTGY
                     GSAFGNSIAKHYPGVDYDDLMASVDAVLKNGYIDQARMYVSGCSGGGVLSSWVIGHTN
                     RFAAAAVRCPVIDWISMAGETDIVYFTYRFFKKPFWEDPTDWLEESSLMHVGNVKTPT
                     LLMTGELDRRTPIPQTEEFYEALKYRGVPAALLRFDGEFHGTGRKPSNWMRTQLYMMS
                     WFDRYGAHTGPPK"
     gene            complement(12172..12657)
                     /locus_tag="VFB37_14205"
     CDS             complement(12172..12657)
                     /locus_tag="VFB37_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HZO23652.1"
                     /translation="MEPFLGWALAISLIVIGTIGTFVPGIPGPVLVFGGMLLAAWIDH
                     FSRVGSITVTILAVLTAIAIAVEIAAGVLGARRVGASRLALLGAAAGTIVGLFFGFVG
                     ILICPFIGAVIGQLMTQPRLAPAVRVGVGTWVGALLGILAKMVIVFAMLLVFLVSYFS
                     S"
     gene            complement(12824..13339)
                     /locus_tag="VFB37_14210"
     CDS             complement(12824..13339)
                     /locus_tag="VFB37_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23653.1"
                     /translation="MSGKAEDTLSLELTDLRVSGGVLWNHLDHGLIANYCEGSWKHRG
                     RYFSRLSVAGGCCLLFGIAREPTLLSEPLELFSFNGSTFHAAGIAVARYNEEQDMWHG
                     LVRPIWWTSMRIVSADAISALVDPANVEILNPWEPSEYRGEPLLSRAVKEAERKQESK
                     IADFPPAHRHD"
     gene            13587..14186
                     /locus_tag="VFB37_14215"
     CDS             13587..14186
                     /locus_tag="VFB37_14215"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZO23654.1"
                     /translation="MIYWHETDTPIGPLLLAGTAEALTRVNFQAGPRPLEPPAQWQHD
                     AAPFAPALTQLREYFSGSRRTFTLPLSPVGTPFQLLVWKGLLSIPYGQTISYGELAQR
                     LGLTSGARAVGLANGANPLPVIVPCHRVIGADGSLTGFGGGLDIKRELLALEGAACVA
                     DLFSETQPGTLSAGERSSSAPQPSAGPTDELGGLRARAP"
     gene            complement(14237..14839)
                     /locus_tag="VFB37_14220"
     CDS             complement(14237..14839)
                     /locus_tag="VFB37_14220"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO23655.1"
                     /translation="MIRLPGKADAASLTAAVALESDPFYRAIAAGPDRPELRQAALAQ
                     YFEYSIREGREAGRCVQLDDPGLGVAVWLLPQPADRAALMATKKRAFLADVLAAEGVR
                     NYYQIVTFMHSRAQRIVAEGDWYLSIVAVNPAVQGQGIGAQLLAPTLAEADRHAISCY
                     LETFSPRSHSFYERLGFKKRAQFREPTTDETYTLMIRAPA"
     gene            complement(14836..16014)
                     /locus_tag="VFB37_14225"
     CDS             complement(14836..16014)
                     /locus_tag="VFB37_14225"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZO23656.1"
                     /translation="MDSPRAPVRARALGIPFEGTPGVHNAITDVPGVEVGQVTRISGE
                     GRLKIGSGPVRTGVTVVLPRGRKSVTPVYGGFFNLNGNGEMTGQSYLQDFGIVIGPIG
                     ISNTNAIGQVYAGIMQWSQKGFGTAATPVVAETSDDWLNDMGGFHIEPADAVEAIDAA
                     KPGPVAEGNVGGGTGMVCFGFKGGTGTASRQVSIPNKHYTVGVLVQCNTGDRTTLRVA
                     GAPVGQHMSKRWLPCYVAELSPNDKQPKCNAKGTAGKPPPDEGSIIIVVATDAPLIPT
                     QLNRMARRAALGLGRLGSYSGNGSGDLVVSFSTAVVANDPDKGHPAQIDPVPNPDLDP
                     LFKAVVEASEEAIVNALVAAQTMTGADGYRFYALPHAELQAILKRYNRLEPATAAVGH
                     "
     gene            complement(16097..17971)
                     /locus_tag="VFB37_14230"
     CDS             complement(16097..17971)
                     /locus_tag="VFB37_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO23657.1"
                     /translation="MGESVSEQEAHSLGLDTRITRRDFVNGSLVAAGAALAGTPRISV
                     GSSDTFTGYGGVGDYANANGNTWPVVQAAHRLRDGGYDAAALAHAQEEGEVELLVVGG
                     GIAGLSAAYYFRKSTHGKRVLVLENHPVPGGEARQNEFLVDGERLLGPQGSNDFAVPV
                     RGSGSATDEFFGEFALPREYRWQTWDPTLKPLRFASDNYSNMDGFQEKQIDVGYYFDQ
                     AQGASGPHWLKNIWSNDLQATPFSAAARRDLLRWRADHGPVSEAESRRLDTLTYKDYL
                     EKDRGYDPAVTRFVEPIVGLLGGVSADAACARLGRQLVESPDRPLALSYPGGNSVFAR
                     ALLRGLLPAALPGTDFSSLMYGRIHFAALEDRSQQMRMRLSATAIRVEHQGGDPGRGR
                     VEVTYEQGGKLYRVRARSVVMASGGWVNRHLLVDMPEDLRAAYEQFAYAPALIVNVAL
                     RQWRFLYDMGVTACRWFDAEGFGYCCNIRRNMVSEGQAPKLHPDRPVVLSFYMGLPVP
                     GLPAAQQGIAARTKFLATPYRDYEVRIRRHMSRLFGASGFKPDRDIAAIVLNRWGHAR
                     LIEPPGFHYGQNGRASPLERVREGYGRIAIGHSELNGAQHWGSAMEYGKKAGEKVASI
                     "
     gene            18049..20076
                     /locus_tag="VFB37_14235"
     CDS             18049..20076
                     /locus_tag="VFB37_14235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZO23658.1"
                     /translation="MPLSALRRVIALHRVIAGLSLAMLAAQAIANPSASANSHSLTPD
                     ELLRVQSVSDPQVSPDGAWVAYVVTSNDLQADEARSAIWMVSWDGAQRLALTGEGADT
                     SRPRWSPDGHYLAYLATPTGSEKSEIMLLDRRGGEASQITGVTADIADYAWSPDGKRI
                     VIAMDQADEAKTPKPIVIEAWHFKQDEEGYLAKGHERHLALLDVQSKRLEALTSDPAF
                     NEDLPVWSPDGSQILFVRTKERPVDRDGREEIALIEPRPGATPHPLLRPFAPNNQKLA
                     WSPDGKLITFLEGLEPRYNAYMQDHLYVVPASGGDPRPLTDKLDRAVQSYTFTSRSKI
                     ALSVEDDRVVYPAELAVSSGAITREGGAEPAVVSELSSAGGHTALIRTTDATPGEVYA
                     LEHGKVRKLTPHNDTWLSSVTLGRAEDIQFKAKDGTEIHGVVIKPPGFVTGRKYPTLL
                     WIHGGPNGQDEHSLGLDGYQFEPQMFATRGFVVLRVNYRGSSGRGIAFAEAIYADWGD
                     KEVADLLAGVDELVSAGIADPDHLAVGGWSYGGILTDYTIASDGRFKVAVSGAGSANQ
                     ISMFGGDEYVLQYTHELGPPWTHTDLWLKVSYPFFHADRIHTPTLFLGGDKDFNVPVI
                     GGEQMYEALRTLGVPAQLVVYPGQHHILTRPSFVKDLAERIPTWIDRYLAR"
     gene            complement(20086..>21308)
                     /locus_tag="VFB37_14240"
     CDS             complement(20086..>21308)
                     /locus_tag="VFB37_14240"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZO23659.1"
                     /translation="SVKASWAGKLAADGQSLSGHWTQRRSLPLDFARQKEAQAAPPIP
                     PATYDPAIAPVEVSEMQAVLTRDLARALKSGMLAPGSSTGVSVGVLRGGVRRVFSYGT
                     AKPDSIFEIGSVTKTFTGLVLAQMIEQGKVKLETPVRELLPPGTVAKAEDHEITLLDL
                     VTQHSGLARMPDNFAPADPNNPYADYHAAELYQFIKQHGLVKPADATFLYSNLGFGLL
                     GQALSNRAAMPYPQLLKEQITVPLGLKDTVVTLTPQQQSRFIPGHNAQHEQVHEWNLD
                     ALGGAGAIRSTADDLLTYLQANLHPESVNTSASANARTLPKAIALSHEVRNTALPGMH
                     IAFAWLREDAKGTYWHNGGTGGYSSFVFFNPQFDFAAVVLVNETLGRTGSFADRIGQH
                     LAQRFAGKPALTLE"
BASE COUNT         3872 a         7139 c         6500 g         3797 t
ORIGIN      
        1 caacaaggcg ctcgcccacg gacagctggc cgctatcgac aaccaggtgg acgtgaccac
       61 cggaaccgtg aagctgaagg cggaatttgc aaacgtcgac gatgctcttt ttccatccga
      121 gttcgtcaac gcgcgactga agatcgagac cctgaccggc cagacgctga ttccgagcgc
      181 cgccgtccag caaggcgccc cgggagccta cgtctatgtg gccaactcga ataacactgt
      241 aacccttcgt aaggtgacga caggtccggc ggccggagag ctggtctcga taacccgcgg
      301 cgtcaacccg ggggagcgcg tcgtcatcga cgggttggac aagctgcgcg acggcggaaa
      361 gatttccgtg gtcgccgctg cccccgggac gccggccacg ccaggtacgc ggcctgcgtc
      421 gccggcatcg aaagcccctg gaacgaccgc cagaaagtcc gctccgaccg cctagctgag
      481 cccatgaatc cttcggcgat cttcatcgag cgcccggtcg ccacctggct gctgatggtg
      541 tccatcctcc tcgccggcat ggtcggctat cacgtcctgt ccgtctcggc gttgccggaa
      601 gtggactacc cgaccatcca ggtgcagacg ctctatccgg gcgcgagccc cgatgtcacc
      661 acttcctcga tcacggcacc tctggagcgg cagttcggcc agatgccggg cctctcgcag
      721 atgacctcct cgagctccgg tggcgcctcg gtgatcacgt tgcagttcag cctggacctc
      781 tcgctcgata ccgcggagca ggaagtgcag gccgccatca acgccgcgag ctcgttcttg
      841 cctgtggatc tgccgatgcc gccggtctac agcaaggtca atccggcgga tgccccggtt
      901 ctcaccctgg cggtgacctc ccccactctg ccgctgacgc aggtcgagga tctggtggac
      961 acccgtctgg tgccgaagct ttcggaagta cccggcgtgg gcctggtgag cctcgggggc
     1021 gggcagcgcc cggcggtacg catccgagtc gatcccaccc tgcttgcttc ccacaacctc
     1081 atgctcgagg acgttcgcac cgccatcggc aatgcgaacg tgaatcaggc caagggcagc
     1141 ttcgatgggc cgctgcgcgc gtacaccatc gatgccaacg accagcttcg cagcgcggac
     1201 gactatcgca acatcgtcct cgcctacaac aatggcgccc cgctgcgcct cggtgatgtc
     1261 gccaccgtcg ttgagcaggc cgagaacgtc aagctcgccg cctggtccgc gtccgccaag
     1321 gatgggaagc tgaccccggc gatcattctc aatattcagc gccaacccgg cgcgaacgtg
     1381 attcagaccg tcgatcgcat caaggcgctg ctgccgcagt tgcagaccag cctccccggc
     1441 tcggtggatg tcgccatgct gaccgatcga accatcagca tccgcgcctc ggtacgtgat
     1501 gttcaattcg agttgatgct ggctgtcgcg ctcgtcgtga tggtcatctt ccttttcctg
     1561 cgcagcgctt cggcgacggt cattccgagc atcgccgtgc ccttatctct ggtcggcacg
     1621 ttcggcgtga tgtacctcgc gggcttctcg ctcaataacc tcacgctgat ggcactcacc
     1681 atcgccaccg gattcgtggt ggacgatgcc atcgtcatga tcgagaacat ctcccgccac
     1741 gtcgaagagg gcatgccccg ccggcaggcc gccttcgaag gctcgcggca gatcggcttc
     1801 accatcatct ctctgacggt ctcgctgatt gccgtgctca tcccgctact gttcatggcg
     1861 gatgtcgtgg ggcggctttt ccggcaattc gccgtgacgc tggccgtcgc aatcctcatc
     1921 tcggccttcg tcagcctgac gttcacgccc atgatgtgcg cgcgcattct cgttccgccg
     1981 gatgagaaga aggagggccg gatcctgcgc acctttggcc gtggcttcga gtccttgagc
     2041 catcactacg gacgcgcgct cacctgggtg ctcgatcacg agcgcctcac cctcatcatc
     2101 gccgtcgcga ccctggtgct gacggcgctg ctgtatgtcc tgattccgaa ggggttcttt
     2161 ccgcctcagg acaccggcgt catccaggcg atcacccagg cgccccctgc agtgtccttc
     2221 tcgcagatgt cacaactgca gagccagctt gcacgcgtga tcctgcagga tccggccgtc
     2281 gacagtatct cttcctatat cggcgtcgat gggaccaaca ccaccttgaa cagcggccgc
     2341 atgctcatca atctggtcgc caagagcgat cgcaatgtcg atgcgagcgg gctgatccgc
     2401 cggctgcagc ccaaagtggc gaacattcag ggcatcacgc tctacatgca gcccgtgcag
     2461 gacctgacca tcgacacgac cgtgagccgg acgcagtacc agctcagtgc ggaggatccg
     2521 aactcaacag agttgagcac ctggatagga cgcctcgtag gcaaactcca gaccgaacca
     2581 accctagccg acgtcgccag cgatctcaac gaccagggct tggaggtcta tgtcgacatt
     2641 gacagggatg cggccggtcg gctcggggtc acggctgccg ccatcgacaa cgccctctac
     2701 gatgcctacg ggcagcgcct cgtttcgacc atcttcacgc aatcgaacct ctatcgggtc
     2761 gtgttggaag tgcggccgcg cgatcagcag gggccggaga aaattcagga cctgtatgtc
     2821 gcgaccgtga acggcaaccg gatcctgctt tcgagcgtcg cccaaatctc ccaacgtatc
     2881 tcgccactgg tcgtcaacca cctcggtcag ttcccggccg cgacgatctc cttcaatctc
     2941 gccccgggcg cctcgctcgg cgaggcggtg aagatcatcc gcaatgatga gaaggcgatc
     3001 gggctgcccg acagcgtaca aaccagcttc cagggcgccg cgcaggcctt tcaggcctcc
     3061 ctcagcaaca ccctgtggct gatcctcgcg gccatcatca ccgtctacat cgtgctgggc
     3121 gtgctctacg agagctacat ccacccggtg acgatcctct cgactctgcc ttctgcgggc
     3181 gtcggggcgt tgctcgccct gatggtggcg ggccaggacc tgggcatcgt cggcatcatc
     3241 ggcatcgtgc tgctgatcgg catcgtcaag aagaacgcga tcatgatgat cgactttgcc
     3301 ctgcaggctg agcggggcga gggcaaggct ccgcgggaag ccatttatca ggccgccctg
     3361 ttgcgcttgc ggcccattct gatgacgacg atggccgctt tgctgggagc gctgccgctg
     3421 atgctcggaa caggcatggg ctcggagctg cgccatccgc tcggcatcac gatggtcggc
     3481 ggcctgctcg tcagccaggt gctgacgctc ttcacgacac ccgtgatcta cctcggcttc
     3541 gagcagcttg cgcagtggcg ccggcggctc aagaagaagc cggacgacga tgcaagccag
     3601 gcgccggtga cggtcacatg aagccggtgg cggtcgcatg aacctgtcac gacccttcat
     3661 cctgcgcccg gtcgcgacga ccctattgac gctgggggtg atcatcgcgg gggtggtggc
     3721 ttttcgactg ttgcccgtct caccgctccc ccaggtggac tttccgacga tctccgtctc
     3781 ggcctccctc ccaggggcca gtcccgacac catggccgct acggtggccg caccgctcga
     3841 gcgctcactg gggcgcatcg cgagtgtcac cgagatgacc tcggccagcg cgctcggctc
     3901 gacgcgcatc acgctgcaat tcgatctgag ccgcgacatc gacggggcag cgcgcgatgt
     3961 gcaggcggcc ctcaacgccg caagggcgct gctgcctacc ggcctgccga gcaatccaac
     4021 ctaccgcaag gtgaacccgg ccgatgcgcc gatcatgatc atcgcgctca cctcgcaggc
     4081 gctttcccgc ggacagatgt acgacgcggc atcgaccgtg ctggcgcaga agctgctgca
     4141 ggtcaatggc atcggcgatg tgaccgtggg aggcagctcg ttgcctgccg tgcgcgtgga
     4201 gctcaatccc gcccggctga atggctatgg cctgagcccc gagacggtgc gcaacgcgat
     4261 cgttgccacc aacccgaacc ggcccaaggg ctccatcgat gacggtgagc ggcgctggca
     4321 gattctcgcc aacgatcagg ctacgacggc aaaggactac atgccgctga tcgttgccta
     4381 caacaacggc aatgccgtcc atctgagtga tatcgccgag gtcgtggact ccgtacagga
     4441 tgtgcgcaac gacggcatct ccaacggtga gcccgcggtc ctcctggtcc tgtttcgcca
     4501 gcccggcgcc aacatcatcg agacggtgga ccgggtcaat gcgctgctgc cgcacctgcg
     4561 agcctccatc ccggccagtg ttgccctcac cgtggtcatg gatcgcacgc cgaccatccg
     4621 cgcttcgctg cgggaagtgg agcgggcgct gatgatttcc gtggcgctgg tcgtgctgtc
     4681 ggtctttcta tttctgcgca gcggccgggc tgcgctcgtg ccgctcgtag cggtgcccgt
     4741 gtcactgatc ggcacgttcg gagtcatgta tctggctggc tacagcctgg acaatctgtc
     4801 cctgatggcc ctgaccatcg ccaccggatt cgtggtcgat gatgcggtcg tggtgctgga
     4861 gaacatctcc cgccacattg aggaaggcat gcagccgttg caggccgcac tgcagggcgc
     4921 acgcgaagtc ggcttcaccg tgctctcgat gagcatctcg ctcatcgcgg tattcatccc
     4981 gatcctgctg atgggcggga tcgtcgggcg gctgttccgg gagttcgccg tgacgctgtc
     5041 gaccgccatc ctggtgtcgc tggtggtctc cctcaccgca acgcccatga tgtgcgcgcg
     5101 gatcctgaga ccccgggacg aggaacccga gcacgggcgc ttttatcacg ccagcgaacg
     5161 cgccttcgat gcgctgcagt ccggctatca ccgctcgctc gactgggccc tcgagcgctc
     5221 acccctgatg atgctgatcc tcgggatcac cattgccctc accatctatc tgtacatcgc
     5281 cattccgaag ggcttctttc cgcagcagga caccggccgc ctgagcggct cgatccaggc
     5341 cgaccagagc atctcgtttc aggccatgca gcagaagttg cgcgacttcg tagccatcgt
     5401 gcgcaaagat ccggcagtca ccggcgtggt cggcttcacc ggcggcggcc agcgcaacag
     5461 cggcaacatg ttcatcgcac tcaagccgct cgccgatcgc aagctctcgg ccgatcaggt
     5521 gatcgcgcgc ctgcgcggca agctctcgca cgaggcgggc gccaatctgt ttctgcagtc
     5581 cgtccaggac atccgggtgg gcggccgctc ctccggggcg cagtaccagt acaccattca
     5641 ggccgacacg accgacgagc tgcgctcctg ggagccgaag atccgcaccg cgctctcgag
     5701 catcaaggac ctggccgatg tcaacaccga tgcgcaggac aagggtctgc agacctcgct
     5761 cgtggtcgat cgtgatgccg tcgcgcgcct cggcctgaat cagaatctga tcgatgccac
     5821 gctgaatgat cttttcggac agcgggaagt gtccacgatc tacaacccgt tgaatcagta
     5881 ccacgtcgtc atggaggcgg cgcccgcctt ctggcagagc ccgaccatac tttccgatgt
     5941 gtacttcacg gcgccgaaca acaagcaggt acccctgggg gatttctcga cctggggcac
     6001 gacctccacg cctctggtcg tcaaccatca gggacagtca gccgcctcga cgatctcgtt
     6061 caacctggcg ccgggcgtat cgctgtcgaa tgccacgaag gatatcgaca gcgcctttgt
     6121 gcggctgcga gcgccggcga ccatccgggg caccttccag ggcaccgcca aggcctttca
     6181 ggatgcgctg gccaaccagc cgctcttgat cctcgccgcc ctggtggcgg tctacatggt
     6241 cctcggaatt ctctacgaga gcctggtgca tcccgtgacg attctctcca cgctgccctc
     6301 ggcaggcgtg ggcgccctcc tcgcgctgct tttgacgggc acggaattca gcatcatcgc
     6361 cttgatcggc gtcatcctgc tgatcggcat cgtcaagaag aacgccatca tgatgatcga
     6421 cttcgccctg cagctcgagc gcgatcagca gctcgagcca cgcgaggcca tccatcgggc
     6481 ttccctgatg cgcctgcgcc cgatcctgat gaccacggtg gcggcaatgt tcggcgccat
     6541 gccgttggcg gtcggcacag gagacggcgc cgagatgcgt cggccgctcg gcatcgcgat
     6601 cgtaggcggc ctgctgctca gtcagcttct tacgctctac acgacacccg tgatctacgt
     6661 ttacttcgac cgcttcagca cgtgggccaa gcgccgcctg catcgttcgc cacagccggc
     6721 tgcgcctgcg cacggtgtcg catgagccga ttgagcacga tgtgccggct caccgcagcc
     6781 cagctggtgc cgacccgcac gcttgcagcc atgctggtgc tcggttgcac gcttgcagcc
     6841 tgctccgtgg gacccgatta caagcggccg aaagtcgaca ctccacctgc cttcaaggag
     6901 gccgagggct ggaaggtcgc cgagccgaat gacacggcgc ccaagggaaa ctggtgggac
     6961 gtgtacaacg acccggcgct cgacaatctg gtcaagcagg tccaggtctc caaccagaat
     7021 gtcatggtcg ccgaggctca gtaccgccag gccctggcgc tcgtcagcca ggctcgcgcc
     7081 aactactttc ccctgatcac ggcggatgcc tcgagcctgc gcagtcagaa tgccctcgtc
     7141 accggagtca gtgggaccgg gacgtcgacc ggcaccccca caacaggcaa tccaggcact
     7201 ggaaccggga ccggcggagg cagccagaat cagacgattc ccggagcgac cgggacgcgc
     7261 aatgtcgacc gggtgagcct gtcgctctcc tgggagatcg acgtctgggg ccggatccgc
     7321 cgccaggtcg aatccaacaa ggccgctgct gtcgccagcg agagcgatct cgccaacgcc
     7381 ctgttgtccg cacaagcgat gctcgttcag agctatgtgc agctgcgggc taccgacacg
     7441 gacatcgatc tctacgagaa atccgtggcg gcatacgaca aggccctcaa gatcaccatg
     7501 aatcgctatg aggcgggggt cgcgcaacgc acggatgtca cgcaggccca gggacaactc
     7561 gagtcggcca aggcggcggg cttcgatctg caggtcacgc gcgcgacgct cgaacatgcc
     7621 attgcggtgc tggtcggcaa gccgccggcg gaactgagca tcgagcgaac gaatcagctc
     7681 cccaacattc cggagacgcc agtgagcgtc ccggccgcgc tcctcgagcg gcggcccgac
     7741 attgctggag ccgagcgccg cgcggccgct gccaacgcgc tgatcggagt ggcgcgcgcc
     7801 gcttacttcc ccgagttgag tctgaccggt tccggagggt atgagtcgaa cactttcaat
     7861 catctcttca gcgtcccgaa tcggttctgg tcactcggcc cggagcttgc gcagactgtg
     7921 ttcgacgctg gggcgatcca cgcgcaggtg aagcagaaca ttgccgccta cgatgcgagc
     7981 gtggcgacct accgtcagac cgtcctcgcg gccttccagg atgtggaaga caacctggcc
     8041 acgctcaaat ggctctccca ggaagcggtc gcgcaagccg ccgccgcagc cgccgctgag
     8101 cagactctca gcatcaccga gaaccagtac caagccggca cggtggacta cctcaacgtc
     8161 gtcatcgccc agcagtcggc tctcaccgcc gagcgggcgg tacgcgacct ggcgaaccgc
     8221 cgcctcaccg ccagtgtggg gctgctcaag gcgctcggtg gcggctggca gaccacgatg
     8281 ctgacgcacg aataggggcc cgaggcagct gccccgatcc gctcccttag acggccggcc
     8341 ctcagtcgcg agcgtgcccg gggaacggtc ggccgttccc gcccccaccg gctcaccacg
     8401 ccggggcctc cacccggcgc gtgctgtctg gacaaaagcc cctgcctgga gcatcgtccg
     8461 cgccctcgcc aagaagactg ctgtcgggga gacaagcatg cgatcgccgg ccctttctgc
     8521 cgttctgacc ctcctcctga tcacgctgtc ctggacgctt ccggccgagg cggcccatcg
     8581 agcccataag acggcggagg ggaaagccga tgcccgcgcc atttccgccg ccatcgccag
     8641 cccggatcgt ccgcaggcgg atcatgagcg ggatgcctgg gcgaaaccgg agctcgtgct
     8701 gaccttcctc ggcgcgcgac cggggatgca tgtcatcgac tacctggcag cggacggcta
     8761 ctacagcgag ctgctcgcgc gagtggccgg gcccaagggg caagtcattg tctacaacaa
     8821 cggggggtat tcgagcttca taggacagaa gctcgccaag cggttcgcag accaccgctt
     8881 gcccaacacg ctcgtgaaag tcaccgagat cgctgacctg aagctgccgg cgaactcact
     8941 ggacgcggcc ttgttcgtca tgtcctacca tgacgtgtac tacacgccca aaggggcaag
     9001 cggtccgatg ggcgatgcgg gtcagatggt gagtgctttg ttcaccgcgc tgaagccggg
     9061 cggtgtcgtg gtggtacagg accacgctgc gcaagccggc accagtcccg ccgagtcggt
     9121 gcagaagctg catcgcatcg atccggaagc cgtgaagaaa accttcgagc aggccggatt
     9181 caagctcgat gcgcaggacg atgcgttcaa gaactccgag gacgatcaca gcaagctcgt
     9241 gttcgacccc tcgatccgcc acaagacaga tgagttcctg tttcgattcc gcaagccagc
     9301 gtaacggcag gccccatgag cttctccctt tacgaggcga cgatcccgag ctatcggcag
     9361 gttctcggag cgatcggggg cctcctggtg aaggcggaag ccttctgtag cgagaagtcg
     9421 ctaccccctc aggagctgat cgaggcccgg ctcgccccgg acatgtatcc gttcgcctac
     9481 caggtcaaat ctaccgcggt ccattcgctg ggcgcgatcg aaggggtgcg caagggcgtg
     9541 ttttcgcccg atgtgacgac ccctcccccg accttcgcag cgctgaaggc tcgcatcgag
     9601 gagacgatca cggcactcga ggccgtgact ccgggggaga tggagggctt catcggccgc
     9661 gacatggcct ttgtgcacgg cgacttccgc atcgagttca cggccgagaa ctttctgctc
     9721 tccttctcgc aggcgaactt ctattttcac gccgcgacga cgtacgacat cctgcgctgg
     9781 aagggagtgc ccatcggcaa gcgcgacttc atgggcaggg tgcgcaagaa gaaatgaggc
     9841 tcgagtcggg ccggcacccc tgccggcccg acacgctgcc attgaattac tttggcggcc
     9901 cggtatgggc gccatagcga tcgaaccagc tcatcatgta cagctgcgtg cgcatccagt
     9961 tcgatggctt gcgcccggtc ccgtggaatt cgccatcgaa gcgtaagagt gctgccggca
    10021 cgccgcgata cttgagcgcc tcataaaact cttcggtctg cggaatgggt gtgcgccggt
    10081 cgagctcacc cgtcatcagc agcgtgggcg tcttcacgtt gccgacgtgc atgagtgaag
    10141 attcctccag ccagtcggtc ggatcttccc agaagggctt cttgaagaag cggtaggtga
    10201 agtagacgat gtccgtctca ccggccatgc tgatccagtc gatgaccggg cagcgcacgg
    10261 cggcggctgc aaagcggttg gtgtggccga tcacccaact cgagagcact ccgccaccgc
    10321 tgcagccgga tacgtacatc ctggcctggt cgatgtagcc gttcttcagg accgcatcga
    10381 cggaggccat caggtcgtcg tagtccaccc ccggatagtg cttggcaatc gagttgccaa
    10441 acgcactgcc atagccggtc gagccgcgcg gattgatgta caggacgaca tagccgttcg
    10501 ccgcaaaatt ctgaaacgat gggttgaatg ccacgctgta catcccatgc gggccaccgt
    10561 ggatctcgag cagcagcgga tacttcttcg aggtgtcgaa gcctgcgggc ttcacgatcc
    10621 aaccctggat gtgggcgccc ccgctcgaat cgacgccgat ctcctcctcg ctcgcgagcg
    10681 ccatcccgtg cagcagctcc tcgttgagat gagtgagctt cctggcggca ttcggtcgac
    10741 ccggattgat gagctcgaca tcgcccggcg agtgaaaatc cgagcgcacg aaggtgactc
    10801 cgttgcggcc tgccgaggca ttctgtacga cctcggagcc gctcgtcagc gcgtgcacac
    10861 ccccgtgcga tgaccacgag tacaggtgca cgttgccgtg atcctcggcg gtgaagtaga
    10921 tcgcgctgct gtccggcgcc cactccaaat gctgtggctc gcggtcaaag ccacccgagc
    10981 gcaaggtctc gttactgcca tccgctgaca tggtccacag atcggcgaca cggtaggaat
    11041 cgccgttctt cgtgaaccct acataggcga tcgtcttgcc atcgggagag agagtcggca
    11101 tccgccagcc gccggtcgtc gtgatcaggc gcttggtcgt tccggtgtcg aggtcgaccg
    11161 aatagatgta gcaatcctgg tcattgcgat cctcatcgcc ctccttgaag ccctcgacga
    11221 tcgccgagcg gccatcgggc gtgaaactcc agtccaccgc atcgctgaac tcatgcacgg
    11281 tgtcgccgat atcccagtcg cccgtggtaa tctcacgggc cgcgccaccg tcggcatcca
    11341 ccaaaaagag atggcggtag ccgcgatccg tcaagcccac accgtcgcgc cgatagtgca
    11401 cccgttcggt gtagcgcggt gcggccgccc actttgcacc ctcaggggcc tccggcagat
    11461 cgatcttcca tttctccggg ctcggtacga ccatggtgaa tccgatgcgg cgcccatccg
    11521 gggaccaatg caggttggcg ggatcgtggt cgagtgtcgt gatctggctg gccggccctg
    11581 gcacattcag atgcagcacg aagatctgct tccctttcgg cttgccttcg ccaccgggcc
    11641 tgccctcacc caggtacgca atgctcttgc tgtcagggga ccacaccgca tcactgccct
    11701 cggcaaggaa ccggtcatgt tgcccctcgg cgtccacgat ccagatggac atgtcgtgtt
    11761 tgtccaatag cttgttgacg accgagcgcg tgtacacgac ctgcgtgcca tcgggtgaaa
    11821 gtcggggaaa gctggccgtc tgcagatcga ggtactttcc gacggtgaac tgcccggaac
    11881 cggaggattc atccgcgtgc aaaggcaacg ccgcataggc gagtacaagt gctgccagag
    11941 tactggccac acggagccgg acgtgagtcg tcatagagcc ttctcccctc gccatcatca
    12001 ggcgtactgc cgcgatgcgc gtcggatttc ttgatgaagt tgatcaggcg cggcagaacg
    12061 cagctcgcag cgcgggtgga gtatatcgcg cgctcggaca ggggacaggc tggcgcaggg
    12121 cttcagaccc ttaagaaatc caaactcccc gaagtggaca gcgtgccgcc gtcatgagct
    12181 gaaatagctc acgaggaaga cgaggagcat ggcgaacacg atgaccatct tcgccagaat
    12241 tccgagcagc gcacctaccc aggtccccac accgacacgc actgccgggg ccagacgagg
    12301 ctgagtcatg agctggccga tcaccgcgcc gatgaaggga caaatgagga tgccgacgaa
    12361 tccgaagaaa agtcccacga tggtcccggc tgccgcaccg agcagggcga gacggctcgc
    12421 gccaacccgg cgtgctccca gcacgccggc cgcgatctca actgcgatcg caatcgcggt
    12481 cagcactgcc aggatcgtca cggtgatcga gccgacccgg gagaagtgat cgatccaggc
    12541 ggcgagcagc atgccgccga acacgagcac gggtccgggt atgccgggca cgaaggtgcc
    12601 gatcgtgccg atcacgatga gcgatatggc cagtgcccag ccgaggaagg gctccatgct
    12661 cgagctcctg gagaatgctg tgttgcgagc atactgcatc gatcgatgca atcggcgccg
    12721 ccacagagcc gcagcgggat cagatcacgg gcacgttgaa accaagcctc gcgtctcgaa
    12781 gcgaggccac gcgtgcgtgg cccacggcaa tcggtttcag gagtcagtcg tgccgatggg
    12841 caggagggaa gtcggcgatc ttcgattctt gcttgcgctc agcctctttg actgcgcggc
    12901 tgagcagcgg ctcaccgcga tattcggatg gctcccaggg attcaggatc tcgacgttgg
    12961 cgggatccac cagagcggag atcgcatccg cgctgacgat tcgcatggag gtccaccaga
    13021 tcggccgcac gagcccgtgc cacatgtcct gttcttcgtt gtagcgcgcc acggcgatgc
    13081 cggccgcgtg aaaggtggag ccattgaagg agaatagctc gagcggttcg gagagcaggg
    13141 tcggctcgcg cgcgattcca aacaggaggc aacagccgcc tgccaccgaa aggcgtgaaa
    13201 aataccggcc gcggtgcttc cagctgcctt cgcagtagtt ggcaatcagg ccgtgatcga
    13261 gatgattcca gagcacccca ccgctcaccc ggagatcagt cagctcgagc gaaagcgtgt
    13321 cctccgcttt tccagacatc gacaacagaa tcaaaggccg ctccttcgtt gccccaccgc
    13381 cactagcaag ccgcgtaccc catgagattc gctcggagat actttgatca acgcgccagc
    13441 atcgcctgct gagcgcatcc gacaagtcgg cgcagcacac cttgaggggt agcgccgccg
    13501 cgatctcgcc gcccgcgtcc gaaaacggcc agtgcgcgaa cgctaacggg cgtagcatcc
    13561 ctgctccaca acgctggagg tgggcgatga tttattggca tgaaaccgat acgcccatcg
    13621 ggccgctgct cctggccggg accgcggagg cactcacacg cgtgaatttc caggccggcc
    13681 ctcggccact cgagccgccc gcccagtggc aacatgacgc tgcgcctttc gcgccggcgc
    13741 tgacacaact ccgcgaatac ttctccggca gccgccgcac cttcacgctg cccctgtccc
    13801 cggtaggcac gccatttcag ctcctggtgt ggaagggctt gttgagcatt ccgtatgggc
    13861 agacgatctc ctatggcgag ctggcgcaac ggctcggtct caccagtggc gctcgcgccg
    13921 tcggtcttgc caacggcgcc aacccgcttc cagtcatcgt gccctgccat cgggtcattg
    13981 gcgcggatgg ctcgctcacc ggctttggcg gaggactcga catcaagcgc gagctgttgg
    14041 cactggaagg cgcagcgtgc gtcgccgatc tgttctccga aacacaacct ggcactcttt
    14101 ccgccggaga acggagctct tccgcacccc aacccagcgc cggaccgact gacgagctcg
    14161 gcggattgcg cgccagagcg ccctgaccgc aacgctcggc gctagtgccg ccccagcagc
    14221 tcgcccccgc cgtccttcag gcaggagctc ggatcatcag cgtgtacgtc tcatccgtcg
    14281 tcggctcgcg aaactgcgca cgcttcttga acccgagacg ctcgtagaac gagtggctgc
    14341 gcggactgaa ggtctccaga taacaggaga tcgcatgccg gtcggcctcg gcgagcgtgg
    14401 gggccagcag ctgtgcaccg atgccttggc cttgcacggc cgggttcacc gccacgatcg
    14461 acagatacca gtcgccctcc gcgacgattc gctgcgcccg cgaatgcatg aaagtcacga
    14521 tctggtaata atttctgacc ccctctgcgg ccagcacatc cgccaggaac gcccgcttct
    14581 tcgttgccat cagcgcggcc cggtcggcgg gctgcggtaa cagccagacg gcgaccccga
    14641 ggccggggtc atcgagctgc acacaacgtc ccgcttcccg gccctctcga atcgagtact
    14701 cgaagtattg cgccaaggcg gcctgccgca gctcgggtcg gtcagggccc gcggcgatgg
    14761 cgcgatagaa tggatcagac tccagcgcga ccgccgccgt gagactggcg gcatctgctt
    14821 tgcccggcaa cctgatcaat gacccaccgc cgccgtcgcg ggctcgagcc ggttatagcg
    14881 cttcaggatc gcctgcaact cggcatgcgg cagcgcgtag aagcgataac catcggcacc
    14941 cgtcatggtt tgggcggcca ccagcgcatt gacgatggct tcctcgctcg cctcgacgac
    15001 cgccttgaag agcggatcga gatcggggtt cggcaccgga tcgatctgag ccggatgacc
    15061 tttgtccgga tcattcgcca cgacagcggt cgagaagctg actacgaggt ctcccgagcc
    15121 gttgcccgaa taggagccga gccgacccag ccccaaggcg gcccggcgcg ccatgcggtt
    15181 gagttgtgtc ggaatcagcg gtgcatcggt ggcgacgacg atgatgatgg atccttcatc
    15241 aggtggcggc ttgccggcgg tgccctttgc gttgcatttg ggttgcttgt cgttcggaga
    15301 cagctccgcg acgtagcacg gcagccagcg cttggacatg tgctgcccca caggcgcccc
    15361 cgctacccgc agcgtggtgc gatcccctgt gttgcattga acgagaactc cgacggtgta
    15421 gtgtttgttt gggatgctca cttgacggga ggcggtgccg gttccgccct tgaacccgaa
    15481 acagaccatg cccgtcccac cgcccacgtt accttccgcg accggccctg gcttcgccgc
    15541 gtcgatcgcc tccacggcat cagcgggctc aatgtgaaag ccgcccatgt cattgagcca
    15601 atcgtcggaa gtctcggcca ccacgggcgt ggccgccgtg ccgaagccct tctgtgacca
    15661 ctgcatgatg ccggcataca cctgaccaat ggcattggta ttcgagatgc cgatggggcc
    15721 gatgacgata ccgaagtcct gcagatagga ctgcccggtc atctccccat tgccgttgag
    15781 attgaagaac cctccataga ctggcgtgac gctcttgcgc ccgcgtggca gaacgactgt
    15841 cacaccggtc ctgacagggc cgctgccaat cttcagccgc ccttccccgc tgatgcgcgt
    15901 cacctggccg acctcgacgc cgggcacatc ggtgatggcg ttatgcacac ccggcgtgcc
    15961 ttcgaatgga ataccgagtg cccgcgcccg caccggtgcc cggggcgaat ccaccgcaga
    16021 tgcagggagc ccgtgcgtat tcaaaaaaag cgcggcgcaa acccaccccg ccaacccctc
    16081 tctgcccgcc ttggacctag atcgacgcaa ctttctctcc agcctttttc ccgtattcca
    16141 tggccgagcc ccagtgctgt gcgccattga gctcagaatg gccgatcgcg atgcgcccat
    16201 aaccctcgcg gacccgctcc aggggactcg ccctgccatt ctggccgtaa tgaaagcccg
    16261 gcggctcgat caagcgcgca tgtccccagc ggttgagaac gatcgccgcg atatcccgat
    16321 ccggtttgaa tccggacgcg ccaaacaacc gcgacatgtg ccggcgtatg cgcacctcgt
    16381 aatccctgta gggtgtggcg aggaacttcg ttctcgcggc aatcccttgc tgagcggcgg
    16441 gcagccccgg taccggcagc cccatgtaga acgacagcac cactggccga tccggatgca
    16501 atttcggcgc ctgcccctcc gacaccatgt tcctgcggat gttgcagcag tatccgaacc
    16561 cctccgcatc gaaccagcga caggcggtga cccccatgtc gtagaggaag cgccattgtc
    16621 gcaaggcgac gttgacgatc agcgccggcg cgtaagcgaa ttgttcgtac gctgcgcgca
    16681 ggtcctcggg catgtccacc agcagatgcc tgttgaccca gccgccactg gccatcacga
    16741 cgctgcgggc ccgcacccga tacaacttgc cgccttgctc ataagtcacc tcgactcggc
    16801 cgcgacccgg atctcctccc tgatgctcga cccgaatggc cgtcgccgag agccgcatgc
    16861 gcatctgctg cgagcgatcc tccaatgccg cgaagtggat ccggccgtac atgaggctgc
    16921 tgaagtcggt tcccggcagc gccgccggta acaaaccgcg caacagcgcc cgcgcaaaca
    16981 ccgaattccc tcccggataa gaaagcgcca ggggacgatc gggggactcg accagctgtc
    17041 tgccgagacg agcgcaggcg gcatcggcgc tcacgccacc caggagcccg acgatgggct
    17101 cgacgaagcg ggtcacggcc gggtcatagc cgcgatcctt ttccagatag tctttgtacg
    17161 tgagcgtatc gaggcgccga ctctcggcct cgctcaccgg cccgtggtcc gcccgccagc
    17221 gcagcagatc gcgccgggcc gccgccgaaa acggggttgc ctgcagatcg ttgctccaga
    17281 tattcttcag ccagtgcggc ccgctcgccc cctgcgcctg atcgaagtag tagccgacat
    17341 cgatctgctt ctcctgaaag ccatccatgt tggagtagtt gtccgaggcg aagcgcagcg
    17401 gcttcagggt cgggtcccag gtttgccagc ggtattcgcg tggcaacgca aactccccaa
    17461 aaaactcatc cgtcgccgac ccgctccccc gcaccggcac ggcgaaatcg ttcgagccct
    17521 gcgggcccag cagccgctcg ccgtcgacga gaaattcgtt ctggcgcgcc tcgccgcccg
    17581 gaaccgggtg attctccagc acgagcacgc gtttgccatg agttgatttg cggaagtagt
    17641 aggccgcact gagtccggcg atgccgcccc cgaccacgag cagttccacc tcgccttcct
    17701 cttgcgcatg cgccagggcc gctgcgtcat aaccgccatc gcgcagccgg tgcgccgcct
    17761 ggaccacggg ccaggtgttg ccatttgcgt tcgcgtaatc ccccacgcca ccgtaaccgg
    17821 tgaaggtgtc gctcgaaccg acgctgatgc gcggcgtgcc ggcgagcgcc gctcccgcgg
    17881 cgaccagcga gccgtttacg aaatcgcgac gcgtaatccg cgtatccaga ccaagggagt
    17941 gggcctcctg ctcgctgacc gattcaccca tggttgaata tccgcccggc gccatttgac
    18001 gcagcaaagc atctcacggg atgatccgcc gtccaatggg ggtctcgcat gccgctgagc
    18061 gcacttcgcc gtgtgattgc acttcaccgc gtgattgcag gcctttcact cgcgatgctc
    18121 gcagcccagg cgattgcgaa cccgtccgcg tctgcaaatt cacattccct gacgccggac
    18181 gagctgcttc gcgttcaatc ggtgagcgat ccgcaggtat cccccgatgg tgcctgggtc
    18241 gcttatgtcg tgacctcgaa cgacctgcag gcggatgaag cgcgcagcgc catctggatg
    18301 gtgagctggg atggggctca gcgcctggcg ttgacgggtg aaggcgcgga cacgagccgc
    18361 ccacgttgga gcccggatgg ccactacctg gcctacctcg ccacccccac gggatcggaa
    18421 aaaagcgaaa tcatgttgct ggatcgccgc ggtggcgaag ccagtcagat cactggcgtc
    18481 accgcggaca ttgcggatta cgcctggtcc ccggatggca aacgaatcgt gatcgccatg
    18541 gaccaggcgg atgaggcgaa gacgcccaag cccatcgtga tcgaagcctg gcatttcaaa
    18601 caggatgagg agggctacct cgccaaaggc cacgagcgtc atctggcctt gctcgatgtg
    18661 cagagcaagc ggctcgaggc cttgacgagc gatccggcct tcaacgagga cctgcccgtc
    18721 tggtccccgg atggcagcca gatcctcttc gtgagaacga aggagcggcc ggtggaccgc
    18781 gacgggcggg aagagatcgc gctcatcgag ccgcgcccgg gcgcgacccc gcatccgctg
    18841 ctgcgtccct tcgcacccaa caaccagaag ctcgcctgga gcccagacgg caagctgatc
    18901 acgtttctcg agggcctcga gccccgatac aacgcctaca tgcaagacca cctgtacgtc
    18961 gtaccggctt caggcggcga tccgcgcccg ctcaccgaca agctcgatcg cgctgtgcag
    19021 tcctacacct tcaccagccg ctcgaagatc gcgctcagcg tcgaggacga tcgagtggtc
    19081 tacccggccg agctggcggt ttccagcggc gccatcacgc gtgaaggcgg tgccgagccc
    19141 gccgtcgttt ccgagctgag ctccgctggt gggcatacgg ccttgattcg gacgaccgac
    19201 gcgacgccgg gcgaagtcta cgcactcgaa cacggaaagg tacggaagct caccccccac
    19261 aacgacacct ggctctcgag tgtcaccctg ggccgcgcag aggacatcca gttcaaggcc
    19321 aaggacggaa cggaaatcca cggcgtcgtg atcaaaccgc cgggttttgt aaccggcaga
    19381 aaatacccga ccctgctatg gattcacggc ggcccgaatg gccaggacga gcactccctc
    19441 gggctcgatg gctaccaatt cgagccccag atgtttgcca cgcgcggctt cgtcgtgctg
    19501 cgggtgaact atcgtggcag tagcggacgc ggcatcgcct tcgccgaggc gatctacgcg
    19561 gactggggcg acaaggaggt cgcagacctg ctcgccggag tcgatgagct ggtttcagcc
    19621 ggcatcgccg accccgacca tctggcggtc ggcggttgga gctatggcgg catcctcacc
    19681 gattacacca ttgcctccga tggccgcttc aaggtcgccg tcagcggcgc aggaagcgcc
    19741 aatcagatct ccatgtttgg cggtgatgag tacgtactgc agtacaccca cgagctgggc
    19801 cccccctgga cccacaccga cctgtggctg aaagtctcct acccgttctt ccatgctgac
    19861 cggatccaca cacccacgct gtttttgggc ggtgacaagg acttcaatgt cccggtgatc
    19921 ggcggcgagc agatgtacga agcgttgcgg actctcggcg tgccggcgca gctcgtcgtg
    19981 taccccgggc agcatcacat tctgacgcga ccgagcttcg tgaaagatct cgccgaacgc
    20041 atccccacct ggatagaccg ctacctcgcc cgctgagccc cgccctcatt ccagcgtcaa
    20101 ggcgggcttg ccggcaaatc gttgcgcgag gtgctggccg atacggtcgg cgaagcttcc
    20161 ggtcctcccg agcgtctcgt tcacgagcac gaccgccgcg aagtcgaatt gcggattgaa
    20221 aaatacgaag ctgctatagc cgcccgtccc accgttgtgc cagtacgtgc ccttcgcatc
    20281 ctcacgcagc caggcgaatg cgatgtgcat cccgggcagt gccgtgttgc gtacttcgtg
    20341 ggacagcgca atcgctttcg gcagcgtgcg agcatttgcg ctcgccgagg tattgaccga
    20401 ttccggatgc agattggcct gcagataggt caacaggtca tcggcggtgg accggatggc
    20461 gccggcgccg cccagggcat cgaggttcca ctcgtggacc tgctcatgct gtgcgttgtg
    20521 cccaggaatg aagcgagact gctgttgcgg tgtcagggtg acaaccgtgt ctttcaggcc
    20581 cagcggcact gtgatctgct ccttgaggag ttgcgggtag ggcatggcgg cgcgattgga
    20641 gagcgcctgg ccaagtaggc cgaagccgag gttgctgtag aggaaggtcg catcggccgg
    20701 cttcaccagg ccatgctgtt tgatgaattg atacagctcg gccgcgtgat agtccgcgta
    20761 agggttgttc ggatcggccg gagcgaaatt gtccggcatc cgcgcaaggc cggagtgctg
    20821 cgtcacgagg tcgagcaacg tgatctcatg atcctcggcc ttggccaccg tgcccggcgg
    20881 caggagctcg cgcaccggcg tctcgagttt caccttccct tgctcgatca tctgcgccag
    20941 caccaggccg gtgaacgtct tggtcacgga gccgatctcg aaaatggagt cgggcttcgc
    21001 cgttccgtag gagaacacgc ggcgtacgcc accgcgcagc accccgacgc tcaccccggt
    21061 tgagcttccg ggggccagca ttccactctt gagtgctcgc gccaggtccc gagtgagcac
    21121 cgcctgcatt tcgctcacct ctacaggcgc tatcgcaggg tcataggtgg ccggagggat
    21181 cggaggagcg gcttgcgcct ccttctgccg cgcgaaatcc agcggtaatg agcgacgctg
    21241 tgtccagtgg cccgacagag attgtccatc cgccgcgagt tttccggccc agctcgcctt
    21301 caccgagg
//
LOCUS       DASRHN010000355         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_952917, whole genome shotgun sequence.
ACCESSION   DASRHN010000355 DASRHN010000000
VERSION     DASRHN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952917"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFB37_14245"
     CDS             complement(<1..840)
                     /locus_tag="VFB37_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO23660.1"
                     /translation="MSRTARPAVARRAARKRPPRVGLVSLGCPKALVDSERIVTRLRA
                     EGYELSADYAGADVVVVNTCGFLNSAKEESLEAIGEAMAENGRVVVTGCLGVEKDRIL
                     TAHPGVLAVTGPHQYEAVVAAVHAAVPPLHDPYVDLVPPEGLRLTPRHYAYLKISEGC
                     NNRCTFCIIPALRGKLASRPAHHVLAEAERLVQAGVKELLVISQDTSAYGLDLGYAES
                     SWRGQPLRARFLDLCRALGSLGAWVRLHYVYPYPHVDDVLPLMAEGKLLPYLDVPFQH
                     ASPR"
     gene            complement(911..1669)
                     /locus_tag="VFB37_14250"
     CDS             complement(911..1669)
                     /locus_tag="VFB37_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO23661.1"
                     /translation="MAKISLTHVSVEIPVFDVSSASLRKVLLGSVGGQFNRTGSQVLV
                     RALRDVSFEAHDGDRIGIIGRNGSGKTTLLRVLSDVYPPTAGSIEITGRVSPMFDATL
                     GMSPDATGMENIRICGMLWGLSDEQIARGIDDVVAFTELGEYVNVPVRTYSTGMFLRL
                     AFAIATLREPEILLLDEVIGVGDSAFFEKAYARLIGMVRQSRILVVASHGEKIIRDFC
                     NKAMWLDGGTLKEFGDVDYVFAAYHKEIARGAAA"
     gene            complement(1684..2541)
                     /locus_tag="VFB37_14255"
     CDS             complement(1684..2541)
                     /locus_tag="VFB37_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO23662.1"
                     /translation="MRPASEAAQASAYVPREHRVVRSAGKRDPLGLDLYEGMVQWPLW
                     TMLGWSDIRQRYRRSTLGPFWITLSTGIFILALGMIYGRLLRMETATFLPYFAVGYII
                     WTFVSSATIDACNAFLEAERIIKQIKRPFSVYVLRVVWRSFVVFLHTAIIFLPLVIFF
                     HVDTGFSILLAIPAFALLLLNQVWVGLVVAILNTRFRDVLQMVTTAVQILFFSTPIMY
                     PVDALGDAAILAHINPVHHFVEIVRAPLLGKAPALESWLVACGTILVGFLVAVLLFRR
                     VERRIVYWL"
     gene            complement(2630..>2878)
                     /locus_tag="VFB37_14260"
     CDS             complement(2630..>2878)
                     /locus_tag="VFB37_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23663.1"
                     /translation="SARLALQATPRNDEVVMAMGARSALDRAVLGLASRLPGRTVTGW
                     VMLAWLWLRLRPYRLVDLVCTAALRRCASALNFVCFSG"
BASE COUNT          528 a          949 c          984 g          417 t
ORIGIN      
        1 gcgcggggag gcatgctgga aggggacgtc gaggtagggc agcagcttgc cctcggccat
       61 cagcggcagc acgtcatcga cgtgcggata cgggtagacg tagtgcagcc gcacccacgc
      121 tccgagcgag cccagtgccc ggcacagatc gaggaacctg gcccgcaaag gctgcccgcg
      181 ccagctgctc tcggcatagc cgaggtcgag cccgtaggcg gaggtgtcct ggctgatgac
      241 gagcagctcc ttgacgccgg cctgcaccag ccgctcggcc tcggccagca cgtgatgggc
      301 ggggcggctc gccaacttgc ccctcagcgc cgggatgatg cagaaggtgc agcggttgtt
      361 gcagccctcg gagatcttca ggtaggcgta gtgccgcggc gtgaggcgca ggccctccgg
      421 cggcaccagg tcgacgtagg ggtcgtgcag cggcggcacc gccgcatgca cggccgccac
      481 caccgcttcg tactggtgcg ggcccgtcac cgccagcacg ccagggtggg cggtgaggat
      541 gcgatccttt tcgacgccga gacagccggt gacgacaacg cgcccgttct cggccatcgc
      601 ctcgccgatg gcttccaggc tctcctcctt ggccgagttg aggaagccgc aggtgttcac
      661 caccaccacg tcggcccccg cataatcggc ggacagctcg tagccttcgg cgcgcagcct
      721 ggtgacgatg cgctccgaat cgaccagcgc cttggggcag ccgagcgaga cgagccccac
      781 gcgcggcggg cgcttgcgtg cggcgcggcg ggcaacggcg gggcgggctg tcctggacat
      841 gcgcgggctt gtagccctcg acggagcgca acgcaaactg gcctcgcagc gccgctgtcg
      901 cggccggcgc tcaggcagct gcgcccctgg cgatctcttt gtggtaggcg gcaaagacgt
      961 agtccacgtc gccgaactcc ttcagcgtcc cgccgtcgag ccacatcgcc ttgttgcaga
     1021 aatcgcggat gatcttctcg ccgtgggagg cgacgacgag gatgcgcgat tggcgcacca
     1081 tgccgatcag ccgggcgtag gccttttcga agaatgccga atcgccgacg ccgatgacct
     1141 cgtcgagcag aaggatttcg ggctcgcgca gggtggcgat ggcgaaggca aggcgcagga
     1201 acatgcccgt cgagtaggtg cgcaccggca cattcacgta ctcgccgagc tcggtgaagg
     1261 cgacgacgtc gtcgattccc cgcgcgatct gctcgtcgga gagcccccac agcatgccgc
     1321 agatgcggat gttctccatg ccggtggcgt cggggctcat gcctagggtg gcatcgaaca
     1381 tcggcgacac gcgccccgtg atctcgatgc tgcccgccgt cggcggatag acgtccgaca
     1441 gcacgcgcag cagcgtggtc ttgccagagc cgttgcggcc gatgatgccg atacggtcgc
     1501 cgtcgtgcgc ctcgaagctc acgtccctga gcgcgcgcac gagcacctgg ctgcccgtcc
     1561 ggttgaactg gccgcccacg ctgccgagca gcaccttgcg cagcgaggcc gagcttacat
     1621 cgaataccgg gatctcgacg gagacatggg tgaggctgat cttggccacg ctcgccgtgc
     1681 tccctacagc caatagacga tgcgccgctc gacgcggcgg aagagcagaa ccgccaccag
     1741 gaagccgacg aggatcgtgc cacaggcgac gagccaggac tccagcgccg gcgccttccc
     1801 gagcagcggc gcccggacga tctcgacgaa gtggtgcacc ggattgatgt gggcgaggat
     1861 cgccgcgtcg ccgagcgcat cgaccgggta catgatcggc gtggagaaga acaggatttg
     1921 caccgccgtg gtcaccatct gcaggacgtc gcggaaccgg gtgttgagaa tggccaccac
     1981 gagaccgacc cacacctggt tgaggagcag cagggcgaag gccggaatgg caagcaggat
     2041 gctgaacccc gtatccacgt ggaagaagat cacgaggggc aggaagatga tggccgtgtg
     2101 caggaagacg acgaagctgc gccacaccac gcgcagcaca tacaccgaga acggccgttt
     2161 gatctgcttg atgatgcgct ccgcctccag gaaagcattg cacgcatcga tggtcgccga
     2221 cgacacgaag gtccagatga tgtagccgac ggcgaagtag ggcaggaagg tagccgtttc
     2281 catcctcagg aggcgtccgt agatcatccc gagcgcaagg atgaatatgc cggtgctgag
     2341 cgtgatccag aacggcccga gcgtggagcg gcgatagcgc tggcggatgt cgctccagcc
     2401 cagcatggtc cacagcggcc actgcaccat tccctcgtag agatcgaggc cgaggggatc
     2461 gcgcttgccg gcactccgca cgacgcggtg ctcgcgcggc acgtacgcgc tcgcctgcgc
     2521 agcttccgac gctggtctca cagcttccat catcccacca tcgcgtctat caagagcgtt
     2581 gtcctgcata accaattctt gcgggctgtc ccgtcggcgc cgccgcgccc tagccggaga
     2641 agcagacgaa attgagggcc gacgcgcagc ggcgcagcgc cgcagtgcag acgaggtcga
     2701 cgagccggta gggacgcaag cgcagccaga gccatgccag catgacccaa cccgtcaccg
     2761 tccgaccagg cagacgcgat gcgagaccca gcaccgcacg atcgagggcc gagcgcgcgc
     2821 ccatggccat gacgacttcg tcgttgcgcg gggtcgcctg cagcgccagc cgggcgga
//
LOCUS       DASRHN010000356         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1453533, whole genome shotgun sequence.
ACCESSION   DASRHN010000356 DASRHN010000000
VERSION     DASRHN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453533"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2078
                     /gene="ileS"
                     /locus_tag="VFB37_14265"
     CDS             <1..>2078
                     /gene="ileS"
                     /locus_tag="VFB37_14265"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZO23664.1"
                     /translation="ERAVYARQREIARGRTRFVLHDGPPYANGAIHIGHAVNKILKDI
                     VVKSRSMDGFDSPYVPGWDCHGLPIELQVEKKHGRPGQKLDATAFRAACRAYAHEQIE
                     LQRKDFKRLGVLGDWAHPYLTMDPAYEAQQLRAFGGIIRNGHLYKGVKPVHWCLDCRS
                     ALAEAEVEYEPKTSPAVDVYFRVVDNAQLEKRVDLAAGRLGQSAVEIVIWTTTPWTLP
                     ANQAVALGPEFRYLLIEAVRDSESHLLIVATELLDSVLQRLQMRARETLAEFPGGALE
                     GLMLEHPFQERQVPVILGGHVTLEAGTGAVHTAPGHGLDDFIVGQRYNLAVVNPVGGD
                     GRFLPDTPLVAGLKVEDANPVLIQVLQERQRLAHHEPYRHSYPHCWRHKSPLIFRATP
                     QWFISMDRQGLRQHTLRDIKKVQWTPAWGEQRITGMIENRPDWCISRQRVWGVPIPLF
                     VNKRSGELHPRTQELIEAAAARVERGGIDAWFALDPAELLHAEADEYDKVTDVMDVWA
                     DSGLSFECVGAQRPELAAPVDLYLEGSDQHRGWFHSSILMSEALYERAPYRAVLTHGF
                     TVDEKGRKMSKSLGNVVAPQKVVSSLGADVLRLWVSATDYANEITVSDEILRRMAESY
                     RRMRNTVRFLLGNLHDFDPARDQVAPAQMVALDRWALIRARELQAEIIEAYRQYTFHV
                     IYQKIHNFCIV"
BASE COUNT          375 a          641 c          668 g          394 t
ORIGIN      
        1 gagcgcgctg tctatgcccg tcagcgtgag atcgcgcgcg gccggactcg tttcgtgctg
       61 cacgatggcc ctccttacgc gaacggtgcc attcacatcg gccatgccgt caacaagatc
      121 ctgaaggaca tcgtggtcaa gtccagatcg atggacggct tcgattcacc gtatgtgccg
      181 ggctgggatt gtcatggcct gccgatcgag ctgcaggtcg agaagaaaca cggacgaccg
      241 ggacagaagc ttgacgccac ggcattccgc gcggcttgtc gcgcgtacgc tcatgagcag
      301 atcgagctgc agcgcaagga cttcaaacgc ctgggcgtgc tcggcgattg ggcgcaccct
      361 tatctcacga tggatccggc ttacgaggcg cagcagctgc gcgcgttcgg cgggatcatc
      421 cgcaacgggc acctctacaa aggcgtcaag ccagtgcact ggtgcctgga ctgccgctcc
      481 gcgctcgcgg aagccgaagt ggaatacgag ccgaagactt ctcctgccgt cgatgtctac
      541 ttccgcgtcg tggacaacgc gcagctcgag aagcgtgtgg atctcgctgc gggcagactt
      601 ggccagtcag cggtcgagat cgtgatctgg acgaccacgc cctggacgct tccagccaat
      661 caagccgtcg ccctgggccc tgagttccgc tacctgctca tcgaggcggt ccgggattcc
      721 gaatcgcatc tgctgattgt cgcgacggag ttgctggaca gcgttctgca gcggctgcag
      781 atgcgtgccc gggaaacgct ggccgaattt ccgggcggcg ccctggaggg cctcatgctg
      841 gagcatccct tccaggagcg gcaggtgccg gtcattctgg gaggccacgt gacactggag
      901 gccggtaccg gagcggtgca cacggcaccg ggtcatgggt tggatgactt catcgtcggt
      961 cagcgataca acctggcggt ggtcaacccc gttggcggtg atggccgctt cctgcccgat
     1021 acgcccctcg tggcgggcct gaaggtggag gatgccaatc ccgtcctcat ccaggtcttg
     1081 caggaacgac agcgcctggc tcatcacgag ccctatcgtc acagctaccc tcactgttgg
     1141 cggcacaaaa gcccgctcat cttccgcgcc actccgcagt ggttcatcag catggatcgg
     1201 caggggctgc ggcagcacac gctgcgcgac atcaagaaag tgcagtggac tccagcatgg
     1261 ggagagcagc gcatcaccgg catgatcgaa aatcggcccg actggtgcat ctcgcgccag
     1321 cgggtttggg gtgtgccgat cccgctgttc gtgaacaagc gcagcggcga gttgcacccg
     1381 cgtacgcagg agttgatcga ggcggcggcg gctcgcgtcg agcgcggcgg tatcgatgcg
     1441 tggttcgcgc tcgatccggc cgaactgctg catgcagaag ccgacgaata cgacaaggtc
     1501 acggatgtca tggacgtgtg ggccgactcc ggactgtcct tcgagtgtgt cggtgcgcag
     1561 cgcccggagc ttgcagcccc tgtggacctt tatctggagg gatccgatca gcatcgcggc
     1621 tggtttcaca gctcgatcct gatgtccgaa gcgctgtacg agcgcgctcc ctaccgtgcc
     1681 gtgctcactc atggcttcac ggtggacgag aagggccgca agatgtcgaa gtccctgggc
     1741 aatgtcgtcg ccccgcagaa ggtagtgagc agtctgggtg cggacgtatt gcgcctttgg
     1801 gtatcggcca ccgactacgc caatgagatc accgtatcgg acgagattct caggcgcatg
     1861 gccgagtcct atcggcgcat gcgcaacacg gtgcgctttc tgctcggcaa cctgcacgac
     1921 ttcgatcccg ctcgcgacca ggttgccccg gcccagatgg ttgctctcga tcgctgggcc
     1981 ttgatccgcg cgcgcgagct gcaggcggaa atcatcgagg cgtatcggca gtacacgttc
     2041 catgtcatct atcagaagat tcacaacttc tgcatcgt
//
LOCUS       DASRHN010000357         8082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_621553, whole genome shotgun sequence.
ACCESSION   DASRHN010000357 DASRHN010000000
VERSION     DASRHN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621553"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1474)
                     /locus_tag="VFB37_14270"
     CDS             complement(113..1474)
                     /locus_tag="VFB37_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO23665.1"
                     /translation="MTATESELRHPPEVPLQFDIGSTDDSLERMTELFARHGDIYRVL
                     VPGRKSYTYVIHHPDDVKRVLVSNHRNYTKGLGIDRVKLLLRKGIMTSEGELWRRQRY
                     MMQPFFHRRVITQFATTVAEPNDRLLTRWEQVARSGEPVNLTDEMSELTLEIVLSSIF
                     GADLPALAEAFDLVTREPARDLQFAYKFRGLTRLVAGVINRRREQREEHADYVGMLMS
                     ARDRDTGQGMSERELIDEIMTLVVAGHETTASGLNWTWYLLSQHPQAEARLHEEIDAA
                     PIEAAPSLGSMESLAYTQQVVNEALRLYPPGWLLSRRAIEADVLGGYDIPAGSNVLLP
                     LYLLHRHPRYWKDPDRFWPERFAPEQEELADVAPFASGSEAPAASAGNPTRRPRFAYM
                     PFAAGPRHCIGETLALYEMLMHLYKVARRFRLIYVPDKPLELEAQINLRTRHPLHMRL
                     ERR"
     gene            1526..2332
                     /locus_tag="VFB37_14275"
     CDS             1526..2332
                     /locus_tag="VFB37_14275"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZO23666.1"
                     /translation="MDGPLTDPWWAAGKAARMSDPGPDGELRNTLYRNTTRSSRRMTR
                     ARRLLYQLAVPVGLTLIRSWMGTCRVVRIAGVEYLEQALARASSLVPCYWHQHQLFCA
                     RFLLDQRGRGLAPGWLISPSVDGELGAMMVRRIGGQVIRGSSTHTGARALRDYYQALV
                     RDGISPVITPDGPRGPRFKFKPGAILLAQMSGRPMLPMAYAASRAWLIKWDKFVIPAP
                     LARIAVAVGPPRYVPRVTDAATLQQLQSEMETELKRLYAQARGMLSGTDS"
     gene            2329..3393
                     /locus_tag="VFB37_14280"
     CDS             2329..3393
                     /locus_tag="VFB37_14280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZO23667.1"
                     /translation="MSLARPLVMRFGAFGDIVLLTVLLRQLHARFGEKVDVIASGPWT
                     RPLLEPQPCLGELFLVRSRRMPYWLSADQHRLVAWLRRRGAGPTWFCDLGLGTELLQR
                     GGIPASLICDSRQFPWQPGESFADRYVRLGNETPCALTGQLPAPIPAVSRAAHLVIDA
                     AARCEGELWLAGRGLRDRPFIVVHPGSRHIARRGLRSRSGASKYWPEQSWGAVLRGLH
                     ERWPEQAILLSGTPAEHGLNEDILACAGVPDAQNAANDLPIRRLLPVLERATGMIAVD
                     TGPAHAAAALGCPTVALFGTAGADLYRPGGATTPAIALTGMVEGRQNILGITPESVLE
                     AWQKLLGTREIPARHILLSP"
     gene            3588..6266
                     /locus_tag="VFB37_14285"
     CDS             3588..6266
                     /locus_tag="VFB37_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO23668.1"
                     /translation="MSSVPTDKPAEFTASDILSADAWTGRLPKPVLGLDVACTKIAVD
                     FQNMRREQIDECTRRNLELLREATATDCAFLIVFGPDELIIEDVRLARSAFAQCQPEG
                     LREAHMDSFPWLKPRLDHLRLSELRDTSAPRREQAAEAQRFAELQIGSALLVSLRVQG
                     RPAAILGLAHTMPRGAWDVNLQLLMKLIGTSLATGLERIRIEARLAKLEERTTLAQLS
                     ANDGLWDFDVQNNEVYFSPRWKSMLGYQDDDIRGSPDWRSLVHPDDLSRVQAAIRDHV
                     AGKTAAFESTHRMRHRNGEWRWVISRATARVDKHGRLLRLVGVELDITERKIYEEALF
                     REKESAQITLQSIGDGVITTDATSTIDYINPVAEELTGWRLEDAMGRPVEEIFRAFHE
                     ETCEPLENPLSVSIRRIRPIKSVRPMLLIRRDGNELYVESTAAPIRDGSGHVAGGVLV
                     FHDVSESRELNRRLSYHASHDLLTGLVNRREFESRLERALKSAKAREASYALCYLDID
                     QFKIVNDTCGHSAGDALLGQVGALLKSKVRWRDTLSRLGGDEFGILLESCSLDEAMRT
                     AETLREGVRNFRFTWEDRVFRLGASIGVVPITADNEDVASILSAADSACQAAKEGGRN
                     RVHSFAENDIELMRRRREMQWAARINAALEEGRFELYRMAIQPLQRIETGAHYELLLR
                     LKDEAGRIVAPNDFIAAAERYGITPAIDRWVIENALRWLVSEADEREKLHMCSINLSG
                     QSLGDDKFLPFVIDQFHRSGLDASKVCFEITETAAVASFSQANRFIQALRELGCKFAL
                     DDFGTGLSSFGYLKHFPVDFLKIDGSFVREILRDPIDREMVRSINEIGHLTGKQTIAE
                     FAENAEIIQMLTSLGVDYAQGYGVAQPQRVLKSAVA"
     gene            complement(6263..7120)
                     /gene="nadC"
                     /locus_tag="VFB37_14290"
     CDS             complement(6263..7120)
                     /gene="nadC"
                     /locus_tag="VFB37_14290"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZO23669.1"
                     /translation="MESQVSEALREDVGTGDVTAQLLPAHQRARGHVITREEAVVCGR
                     PWVDETFRQLDPQIRLDWKVAEGARIVPDQTLFEIEGLARPILTGERTALNFLQLLSG
                     TATAARRFADAVAGTGCSILDTRKTLPGLRTAQKYAVRQGGGRNHRIGLYDLLLIKEN
                     HIAAAGSIPAAVAAGRRTGGELQIEVEVESLQEFSAALDAGADIIMLDEFSLADMKAA
                     VQINRARAHPVKIEASGSVSLERVREIASTGVDYISVGAITKHVRAVDLSMRFDQSEA
                     GAGFAPRRA"
     gene            complement(7176..7787)
                     /locus_tag="VFB37_14295"
     CDS             complement(7176..7787)
                     /locus_tag="VFB37_14295"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZO23670.1"
                     /translation="MNPLNSVSGSIWTGFILAVIVLLIAMAVHMNTGSFFEPTLARWL
                     HILSGITWIGLLYYFNLVQTPGMAVAAADKGGPGGAGVSKYIAPRALWWFRWGAVATW
                     LTGAWYLTRGSNFVNALTLGHGAQDRYGFTIGIGAWLGTIMLFNVWALIWPNQKRILG
                     IVAATDEEKAKARKTAGLASRVNFVLSIPMLLCMASAFHQFPL"
BASE COUNT         1366 a         2579 c         2687 g         1450 t
ORIGIN      
        1 agctctccga aggagacgcg ccgcgcctcg ttctcgcctt cgaggtaggt gaccgcgcgg
       61 tccaccgtgc gattgctttc gatgaggtcc gtgagtgtcg tggcttgcat catcagcggc
      121 gttccagcct catgtgtagg ggatggcgcg tgcgcaggtt gatctgcgct tcgagctcca
      181 ggggcttgtc aggcacgtag atcaggcgaa agcgccgtgc gaccttgtac aggtgcatca
      241 gcatctcata gagcgcgaga gtctcgccga tgcagtggcg cgggcccgcc gcaaagggca
      301 tgtaagcgaa gcgcggccgc cttgtcggat tgccggcaga cgccgccggc gcttccgatc
      361 cgctggcgaa aggcgcgacg tccgcgagct cctcctgctc cggtgcgaag cgctcgggcc
      421 agaaccggtc tggatccttc cagtagcgcg gatgcctgtg cagcagatac agcggcagca
      481 gcacgttgct gccggccggg atgtcgtagc cgccgagcac gtccgcttcg atcgcacggc
      541 gcgacagcag ccatccgggg ggatacagcc gcagcgcctc gttgaccacc tgctgcgtgt
      601 aggcgaggct ttccatgctc ccgagactcg gcgccgcctc gatcggggca gcatcgatct
      661 cctcgtgcag gcgggcctcg gcctgcggat gctgcgagag caggtaccag gtccagttga
      721 ggccgctggc ggtggtctcg tgccccgcca cgaccagcgt catgatctca tcgatcaact
      781 cccgctcgct catgccctgg ccggtatccc ggtcgcgggc actcatcagc atcccgacat
      841 agtcggcgtg ctcctcgcgt tgctcgcgcc gtcggttgat caccccggcc accagccgcg
      901 tcagaccgcg gaacttgtag gcaaactgca gatcgcgcgc gggctcgcgc gtgacgagat
      961 cgaaagcctc ggcgagggcc ggcagatcgg cgccgaagat cgagctcagg acgatctcca
     1021 gggtcagctc gctcatctca tcggtgaggt tcaccggctc gccgctccgg gcaacctgct
     1081 cccagcgggt cagcagccga tcattcggct ccgccacggt cgtggcgaac tgggtgatga
     1141 cccgccgatg gaagaaaggc tgcatcatgt atcgctgccg cctccacagc tctccctcgc
     1201 tcgtcatgat gcccttgcgc aggaggagct ttacgcggtc gatcccgagg cccttggtat
     1261 agttgcgatg gttcgagacg agcacccgct tcacatcatc cggatgatgg atgacatagg
     1321 tgtaggactt gcgccctggt acaagcacgc ggtagatgtc cccgtgccgg gcaaagagct
     1381 ccgtcatgcg ctccagggag tcatccgtgg agccgatgtc gaactgaagc ggtacctcgg
     1441 gtgggtgtcg cagctcggac tcggtagcgg tcatgcaggc gccagatggg caaaaccgta
     1501 atcttatctt acccgcaacc gccccgtgga tggccccctg accgacccgt ggtgggccgc
     1561 aggcaaggca gcgcgcatga gtgatccggg gccggacggc gagcttcgca acactctcta
     1621 ccgcaacacg acgcgctcca gccgccgcat gactcgcgcg cggcgcctgc tgtatcagct
     1681 tgccgtgccc gtgggattga cgctcatccg cagctggatg gggacctgcc gggtggtgag
     1741 gatcgccggt gttgagtatc tcgagcaggc gctcgcgcga gcctcctcgc tggtgccgtg
     1801 ttactggcac cagcatcagc tcttttgtgc ccgctttctg ttggaccagc ggggacgcgg
     1861 actcgctccc gggtggctga tcagtccatc ggtcgatggc gagctcggtg cgatgatggt
     1921 ccgccgcatc ggcggccagg tcattcgcgg ctcatcgact cacaccggtg cgcgcgccct
     1981 gcgcgattat taccaggccc tggtgcggga tgggatctcc ccggtgatca ccccggatgg
     2041 tccgcggggg ccgcgcttca agttcaagcc gggcgcgatc ctgctcgcac agatgtcggg
     2101 ccgcccgatg ttaccgatgg cctacgcggc ctcgcgcgcc tggctcatca agtgggacaa
     2161 gttcgtcatt cccgcgccct tggcacgaat cgcagtcgct gtcggcccgc cgcggtatgt
     2221 gccgcgggtc accgacgccg cgacgctgca gcagctgcaa agtgagatgg aaacggagct
     2281 gaaaagactg tacgcgcagg cccggggcat gctgtcaggg accgactcgt gagtctggcg
     2341 cgcccgctgg tcatgcgctt cggggcgttc ggcgacatcg tgctgctcac tgttcttctg
     2401 cggcagctgc atgcgcgctt cggggagaag gtggatgtga ttgcatccgg cccctggacg
     2461 cgaccgctgc tcgagccgca accgtgcctg ggcgagctgt tcctggtccg cagccggcgc
     2521 atgccttact ggctgagcgc cgatcaacac cgcctggtcg cctggctgcg ccgccgcggg
     2581 gccggcccga cctggttctg cgatctgggg ctcgggaccg agctgctgca gcggggaggc
     2641 attcccgcga gtctcatctg cgattcgcgc cagttcccct ggcagccggg tgagagtttt
     2701 gcggaccgct acgtgcggct ggggaacgag accccttgtg cattgacggg gcagttgccc
     2761 gctccgatcc cggcggtgtc gcgtgcggca catctggtca tcgacgcggc agcgcggtgt
     2821 gagggggagc tctggttggc ggggcgcggt ttgcgcgata ggccttttat cgtcgtgcat
     2881 cctggcagtc ggcatatcgc ccgtcgcggg ctgcggtcgc gctcgggtgc ctcgaagtac
     2941 tggcccgagc agagctgggg agccgtgttg cggggtttgc acgagcgatg gcccgagcag
     3001 gcgatcctgc tctcggggac cccggccgag catggactga acgaggacat cctcgcttgt
     3061 gcgggtgttc cggacgctca gaatgcggca aacgatctgc ccattcgccg tctgctgccg
     3121 gtcctggagc gtgcgacggg catgatcgcc gtggataccg ggcccgcgca cgccgctgcc
     3181 gcattgggct gcccgacggt ggcgctcttc ggcactgccg gtgcggatct ttatcgtccc
     3241 ggcggggcca ccacaccggc gatcgcgctg accgggatgg tggagggccg gcagaacatt
     3301 ctcggtatca ctccggagtc ggttctcgag gcgtggcaga agctgctcgg cacccgtgag
     3361 attccggcgc gacacattct gttaagtccc taacctccgc tcgaacagta cgttagcctg
     3421 tgttcttgtg ttagtgtgcg agaaccgcgc gcaacccact aaattctaga ggggcctgag
     3481 acttcttgca gacggccgaa tctgcaagct aacccctaca gcgtcacatc ggaggggttg
     3541 cccagtcagg taggctgagc gggtcgattc gggaggtttt caggtacatg tcctctgtcc
     3601 ccacggacaa gccagcggaa ttcaccgcgt cggacatcct ttccgcagat gcctggacgg
     3661 gacgccttcc caaacccgtg ctcgggctgg atgtcgcctg cacgaagatc gccgttgatt
     3721 tccagaacat gcgccgggag cagatcgatg agtgcacgcg gcgcaatctc gagttgctgc
     3781 gcgaggcgac ggccaccgac tgcgcctttc tgatcgtttt cggacccgat gagctcatca
     3841 tcgaggatgt gcggctggct cgcagtgcct tcgcccagtg tcagcccgag gggctgcgcg
     3901 aagcccacat ggattccttc ccctggctga agccacgcct ggatcatctg cgtctgtccg
     3961 agctgcgcga tacgagcgcg ccacggcgcg agcaggcggc cgaggcccaa cgcttcgcgg
     4021 agttacagat cggctcggcc ctgctggtca gcctgcgggt gcagggcagg ccggctgcga
     4081 tcctcgggct cgcgcacacg atgccgcgtg gggcctggga cgtgaatctg cagctgctca
     4141 tgaagctgat cggcacgagc ctcgccacgg ggctcgagcg catccggatc gaagcgcggc
     4201 tcgcgaagct cgaggagcgc accactctcg cgcagctctc cgccaacgat gggctgtggg
     4261 acttcgacgt ccagaacaac gaggtgtatt tctcgccgcg ctggaagtcg atgctcggtt
     4321 atcaggacga tgacatccgc ggctcgccgg actggcgcag cctggtgcat cccgatgatc
     4381 tgtcgcgcgt ccaggcagcg atccgagacc atgtggccgg caagactgcc gctttcgaga
     4441 gcacgcatcg catgcgccac cgcaatgggg agtggcgctg ggtcatcagt cgcgccacgg
     4501 cgcgggtgga caagcacggc cggctgctgc gactcgtcgg ggtggagctg gacatcaccg
     4561 agcgcaagat ctacgaggaa gcgctgttcc gcgagaagga gagcgcgcag atcacgttgc
     4621 agtcgatcgg cgatggggtc atcacgaccg atgccacatc caccatcgac tacatcaatc
     4681 cggtcgccga ggagctcacc gggtggcggc tcgaggatgc gatggggcgc cccgtcgagg
     4741 agatcttccg ggcgttccat gaggagactt gcgagccgct cgagaatccc ctgagtgtct
     4801 cgatccgccg gatccgtccg atcaagtccg tacgtccgat gctgctcatc cgccgcgatg
     4861 gcaacgagct gtacgtggag agcacggcag caccgatccg tgatggctcg ggacacgtgg
     4921 ccggtggggt gctggtgttt cacgatgtca gcgagtcgcg cgagctcaat cgacgcctgt
     4981 cctaccatgc gagccacgat ctgctcaccg gtctggtgaa ccgccgcgag ttcgagagca
     5041 ggctcgagcg ggcgctgaag agtgcgaagg cgcgcgaggc ctcctacgcg ctgtgctatc
     5101 tcgacatcga tcaattcaag atcgtcaatg acacctgcgg ccacagcgcc ggcgatgcgc
     5161 tgctggggca ggtgggcgcg ctgctgaaat ccaaggtcag gtggcgcgat accctctcac
     5221 gcctcggcgg agacgaattc ggaatcctgc tcgagagctg ctcgctggat gaggcgatgc
     5281 gcacggccga gaccctgcgc gagggtgtcc gtaacttcag gttcacctgg gaagatcggg
     5341 tctttcgtct cggcgcgagc atcggcgtgg tcccgatcac ggctgacaac gaagacgtcg
     5401 cctccatcct ttcggcagcc gacagcgcct gtcaggcggc caaggagggc gggcgcaacc
     5461 gggtgcacag ctttgccgag aacgacatcg agctcatgcg ccggcggcgg gaaatgcaat
     5521 gggccgcgcg catcaatgcc gcgctcgagg aagggcgctt cgagctgtac cgcatggcga
     5581 tccagccgct gcagcggatc gagaccggcg cacattacga gctgctgctg cgcctgaaag
     5641 acgaggccgg ccgcatcgtc gcgccgaacg atttcatcgc agcggccgag cgctacggca
     5701 tcaccccggc gatcgaccgc tgggtgatcg agaatgcctt gcgctggctc gtgtcggagg
     5761 ccgacgagcg cgagaagctg cacatgtgct cgatcaatct ttcgggccag agccttgggg
     5821 acgacaaatt tttgccgttc gtcatcgacc agttccaccg cagtgggctc gatgcatcca
     5881 aagtgtgctt cgagatcacc gagacggcgg cggtggcgag cttctcgcag gcgaaccgct
     5941 tcatccaggc tctgcgggag ctcggttgca agtttgcgct cgatgacttc ggaacgggcc
     6001 tgtcctcgtt cggctacctg aagcactttc cggtcgactt cctcaagatc gacggcagct
     6061 tcgtgcgtga gattctgcgc gacccgatcg atcgtgagat ggtgcgctcg atcaacgaga
     6121 tcggccatct cacgggcaag cagaccatcg cggagtttgc cgagaacgcc gagatcatcc
     6181 agatgctcac gagcctcggc gtcgactacg cgcagggtta tggggtcgcc cagccccagc
     6241 gcgtgttgaa atcggctgtt gcctaggcgc gccgcggcgc gaaacccgcg ccggcttccg
     6301 actgatcgaa tctcatcgag agatccaccg cgcgcacgtg cttggtgatt gcgccgacgg
     6361 agatgtagtc cacgccggtc gaggcgattt cgcgcaccct ctccaggctc acgcttcccg
     6421 aggcctcgat cttcacagga tgtgcccggg cccgattgat ctgcactgcg gccttcatgt
     6481 ctgcgaggct gaactcatcg agcatgatga tgtcggcgcc ggcatcgagc gcggccgaga
     6541 attcctgcag cgattcgact tcgacctcga tctgcagctc accgccggtc cggcggccgg
     6601 ccgccacggc cgcggggatc gatccggcag cggcgatgtg gttctccttg atcaacagca
     6661 gatcgtacag accgatgcga tgattgcggc cgccaccctg tcggaccgcg tacttctgcg
     6721 ccgtgcgcaa gcccggcagg gtcttgcgcg tatcgagaat ggagcagccg gtgccggcga
     6781 ccgcatcggc gaagcgtcgg gcggctgtcg cggttccgga cagtaactgc aggaagttga
     6841 gcgcggtgcg ctcaccggtc aggatcgggc gggcgagccc ctcgatttcg aacagtgtct
     6901 gatcggggac gatccgagcg ccttcggcca ccttccagtc gaggcgtatc tggggatcga
     6961 gctgccgaaa ggtctcatcg acccaagggc ggccgcacac aacggcttcc tcgcgcgtaa
     7021 tcacgtgccc gcgcgcgcgc tggtgagccg gcaagagctg ggccgtcaca tctcccgtgc
     7081 cgacgtcctc gcgcagagcc tcactgacct gtgactccac atccgccggt acatcatcga
     7141 gcgcgagcga gtcacgccag ctcagaatgg cctcctcaca aggggaactg atggaatgcc
     7201 gaggccatgc acagcagcat cggaatggag agaacgaagt tcacccgcga ggcaagtcca
     7261 gcggtcttgc gcgccttcgc cttctcctcg tccgtggccg caacgatgcc gagaatcctt
     7321 ttctggttcg gccagatcaa ggcccaaacg ttgaacaaca tgatcgtgcc cagccacgcg
     7381 ccgattccga tggtgaaccc gtagcgatcc tgtgcgccgt ggcccaaggt cagcgcgttg
     7441 acgaaattgc tcccgcgcgt gaggtaccag gcgccggtga gccaggtcgc caccgcgccc
     7501 caacggaacc accacagtgc ccgcggggca atgtatttac tcacgccggc tccgccgggt
     7561 ccgcccttgt cggctgcagc caccgccata cctggagtct gcaccaggtt gaagtagtag
     7621 agcagaccaa tccaagtgat gccgctgagg atgtgcagcc agcgtgcaag cgtaggctcg
     7681 aagaacgagc cggtgttcat gtggaccgcc atggcaatga ggagcacgat caccgccagg
     7741 atgaaacccg tccagatcga gcccgagacc gaattgaggg gattcatgtt cgctccaact
     7801 tgttgttctg actgggtgaa gctaccgccg cgcccaagtt tgaagaatag gtgaggaccc
     7861 cccataattc aatggcaatc cggcgcggcc gcggtggcag ggtcgcaagg gtgcgctagg
     7921 cgttgcgcgc gggtgcggcg cccaagctgg agcggcacct aaggtggaac gagtgcatca
     7981 cagtggcggg cccgaggatg gccagcggcc cccctctccc tgcgtgaata tctgcgagct
     8041 ggatgatgcg ggcttctgca tcggctgcct gcgcaccggg ga
//
LOCUS       DASRHN010000358        18812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1606452, whole genome shotgun sequence.
ACCESSION   DASRHN010000358 DASRHN010000000
VERSION     DASRHN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18812
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606452"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VFB37_14300"
     CDS             complement(<1..1483)
                     /locus_tag="VFB37_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23671.1"
                     /translation="MELLLNRFSELAAPLARRILRVASLAGFAAASVNASAQDSGPGF
                     PLTLDPAGVLESTSTNGFHTGVTNGFFTRLGTNGRTCATCHVEADAWALTPRHAQQLA
                     HNDSLFAPVDGSDCPATSGNQAPNRANSSMLLRYAVIRVQLPIPATADFSLVAATNPA
                     GCAIAPGSAAISGNLFLFRRPLPSTNLLLLSSMMWDGRETLQKITTQTGDNGSSAALL
                     FDLDDQANSATIGHAQGAPIAGMPAQQDIVSFEKGLSTAQVVLVPETGVALLSVGANG
                     GAYYLQNTVTPAFSIGVNDPLNPPFTNTVFTIYSAWEPGSPEYRHLSPVQQAIGRGEA
                     IFNNTTFVVHDVPGINSIPSDPLYNAADPLAGQDVVAGCGLCHNNPNVGNHSTSLPIN
                     IGLTMAHPTNNNGSANQALDLANLPVYTLTNSSTGATVQVTDPGRALITGNWKDIGKT
                     KGPILRGVAARAPYFHNGSAKDLATVVTFYNQRFNIGLTSQQQS"
     gene            1708..2574
                     /locus_tag="VFB37_14305"
     CDS             1708..2574
                     /locus_tag="VFB37_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZO23672.1"
                     /translation="MLVFATFLRSCIAAPAAVIMSLVLLCSAAPAMGAEQAAPIRLYT
                     LDGGTIEFKDLASFSDTGDYDGKPGTVAVPCFLIRHPKGTLMWDTGLSAAFAKDDPWK
                     KYGVHATVGASIEQQLKQIGLTPADITYVAFSHLHMDHIGNANLFAGSTWILNKNEVA
                     WASRADAPAGDLSTFSAYKGAKTKMIEGDYDVFGDGTVEILKTPGHTPGHQVLLLRLK
                     SGNVLLSGDLWHLRSDIAGHIVYSGNFDRAQTLASFDRVEKILKNRNARLIVQHDPQD
                     YQGLPKIPAFLE"
     gene            complement(2633..6061)
                     /locus_tag="VFB37_14310"
     CDS             complement(2633..6061)
                     /locus_tag="VFB37_14310"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZO23673.1"
                     /translation="MRLPFTTLLFERLVLGLVMAGLAGLADAQSSGHTLMRYPTLYGQ
                     TIVFVAHDNLWAAPRTGGVASRLTADEGRDLMPRFSPDGRWLAFTGEYQGNRDVYVMP
                     SAGGPARRLTFTSDVTEEAPLRWGPNNMVVTWKPDSSGIVFLSRRQSWNTWFGKLYSV
                     PLSGGLPTALPLDRGGFMSYGPGGTQIAYVRILSDFRTWKRYHGGLNQNIDIYDFRTH
                     ELKHVTDWSGTETSPMWFGKIIYFLADHDQAERRNIWAYDTSNGQFRQITHFTDYDID
                     FPSLGTGPQGEAGIVFQQGGRLHVIDLPSEQLHDLEITVPDDGTRTGPHWVDAKSTIR
                     DQDTAQQTDFDLAPNGGRAVFSARGDLYTLPAEHGNTRNLTQTSGADEDHPAWSPDGK
                     SIAYTTDVTGEQQIAVRPAEGGAERVLTHFERGYFYRPRWSPKGDHLAFSDHEHRLWF
                     VGSDGGAPVQVAQDKFNEIHDYTWSPDGRWLAYSITGENQQSGIWLYNLEKRKATLIS
                     DPRFNDFQPAFAPDGKYLYFISTRHENPTFSRTEFNIATLKMTGIYVATLKRGTASLF
                     APRSDEGVPKEDHEASSDEHKESAEGQKESSEDQKDKKSSGKKRPSAASVEIELDGLM
                     TRAVPMPITPADIASFDVRDDKVFYLTTPSPMIEGPLPGEKPVLHVYDLKKRKDENIV
                     DGLSAYALSDDGKKVLYKLDKDYYIADAAPGKDKDEDAKKKLDLSHMKVRVEPTREWA
                     EMFNSAWRLERDLFYSPQMNGVNWPAVHESYGKLLPLVGSRSDLNYLIGEVIGELSNS
                     HTYVGGGDDMPEERRVPTAFLGADFALEDSSGRYRLATILPGDNTHDAYRSPLTAPGL
                     DVHAGDYLLAIDGTELKAPMDPFSLLVGKQDSTVKLTVADSPGGKRRDIVVQPVKNEV
                     DLREQAWVDHNREVVDQASGGRIAYIYLSDMGGRGMDQFIRQFYAQLDKQALIFDDRW
                     NGGGFIDQIVLERLRRILIGMDTNREHAAIPIPNQLIIGPKVCLLNHYSASDGDIFPF
                     FFRQYGLGPLIGTRSWGGVRGIRGEWTLLDGGYITVPESAQYLLNSQWAIENHGVDPD
                     MTVEDSPADWMQGHDVQLEAAIHYILGEMQKHPATLPPPPPALPAYPKEGGPGL"
     gene            6213..6761
                     /locus_tag="VFB37_14315"
     CDS             6213..6761
                     /locus_tag="VFB37_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZO23674.1"
                     /translation="MNFCNQCGAKVEFRVPPGDHLPRYVCPACGVIHYLNPRLVVGCV
                     PDHEGKILLCRRAIEPRRGFWTVPAGFMENGETLQEAAARESYEEALARVEIGSLLSV
                     VHVLHAHQVHVFFRARLTEPTFGVGAESLETLLLEPEAIPWSEIAFPSTDFTLRHYLE
                     DRAAGREGHYFTQFDRRLPRQG"
     gene            6849..7172
                     /gene="fdxA"
                     /locus_tag="VFB37_14320"
     CDS             6849..7172
                     /gene="fdxA"
                     /locus_tag="VFB37_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HZO23675.1"
                     /translation="MTFVVTENCIKCKYMDCVEVCPVDCFHEGPNFLTIDPDECIDCT
                     LCEPECPIEAIFSEEELPQGQEHFKQLNAELAKVWPVITEKKEPPADAKSWEGKPDKL
                     KLLEK"
     gene            complement(7250..7975)
                     /locus_tag="VFB37_14325"
     CDS             complement(7250..7975)
                     /locus_tag="VFB37_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZO23676.1"
                     /translation="MPARPSLLLILTLLAPALHAQPAAQEDPRAQIASKIPGTHPDDV
                     HPSAVTGIYEVSRGMEFAYVTADGKYAFSGDLIDLNANDNLTEERRRTARQKLIEAIP
                     ESDMIVFGPRDPKYTVTVFTDVDCAYCRELHLHIADYNRLGIRVRYLSYPRTGPNTES
                     WTKAEEVWCSRNRNDALTAAKRGMALTTPPCAINPVAREYALGRTFDLKGTPAIVTAD
                     GEMFDGYVPPDTLAAHLREAPKR"
     gene            complement(7992..8897)
                     /gene="xerD"
                     /locus_tag="VFB37_14330"
     CDS             complement(7992..8897)
                     /gene="xerD"
                     /locus_tag="VFB37_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZO23677.1"
                     /translation="MTAHADSADPAVSRFLDAVWMERGLSPNTLAAYRADLTALSRWL
                     AERDVPMMRTTRADLQDFIAWRVNAGARPRSTARQLSSFRRFFRYLVREGVIREDPTA
                     QIAMPKIGRSLPKSLTEEEVESLLNAPVVSDPLGHRDRAMLEVLYATGLRVSELVNLR
                     HGQVNTNQGVIRILGKGNRERLIPLGEEAMRWLTEFASSARSEILLERQTDYLFPTRR
                     GDRMTRQAFWHIIKRYARKSGISKELSPHTLRHAFATHLLNHGADLRVVQMLLGHSDL
                     STTQIYTHVARERLKELHSQHHPRG"
     gene            9159..9959
                     /locus_tag="VFB37_14335"
     CDS             9159..9959
                     /locus_tag="VFB37_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZO23678.1"
                     /translation="MTAALRILLAPVRIAYGLYVLAVFFVTGSCAFALTVILPGLERR
                     RAVARFSSLAFLWLAGMPLTVRSASHLPRGQCVVVSNHASYLDGVVFTAALPPRFGFV
                     IKREMAGVPLAGLLLRRLGSEFVERFNRHQGAADARRMLRRAVEGNSLVFFPEGTFTR
                     TPGLLKFHTGAFVTAARAGCPVVPAIVRGSRRALSPQGKLPRPGRIIVEILPAVALTT
                     KDPATLPMDLRERSREAILRALGEPDLAGSHPHDPAPERPAQSLEQDA"
     gene            complement(9994..13458)
                     /locus_tag="VFB37_14340"
     CDS             complement(9994..13458)
                     /locus_tag="VFB37_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZO23679.1"
                     /translation="MNASTEGGTALLRPEQSAAQPPLRFVSAASLFDGHDAAINMIRR
                     LLQAHGAEVVHLGHNRSVADIVRAAIQEDADAVAVSSYQGGHNEYFEYMVNMLRERDC
                     EHIRVIVGGGGTIAPHEIEVLERHGVEKIYTPEDGRRLGLNGMIDDVYRRLKSVTKPP
                     YEFGPLNARDQRHIARTISILERAGEADPQAAQIRRALARSRHQVPVIGITGTGGAGK
                     SSLSDELLTRFVRQFPGRQIAMVAMDPTRRRSGGALLGDRIRMNSLASESIFMRSLAT
                     RRQHLATAAVLREVIELYKAAGFDLIVVETAGIGQSDTEIVDLVDLSLYVMTSEYGAA
                     SQLEKIDMIDFANFIVLNKSEKRGAEDSLRDVRKQWRRNHPEQARIADAEVPVYSTIA
                     SRFNDPGVNRLFAALCRALDEKKVGAESWQVTDVGPTELTKREPLIPGQRIRYLAEIA
                     QHGRTSRQRAEEQAEAARRASGIYEALQALQDPALPAPLERFADSALLEHADATRQQL
                     RRTYNRSLDEIGAEGIGELKAWPERVRAATDATYAYKVRNATVSGENYTESLSHQRIP
                     KIAVPKFRDWGEILRFVATENLPGSFPYTGGVYPYRREQEDPTRMFAGEGPPERTNRR
                     FHYLAGGHNAARLSTAFDSTTLYGEDPDTRPDIYGRTGNSGVSIATLDDMKKLYSGFD
                     LCRSNTSVSMTINGPAPMILAMFMNTAIDQQVERYLRADGRWEAAQARITALIGAHTA
                     GGIAPPAYKGELPKGHDGSGLALLGVTGDQLLERETYERIKADALQAVRGTVQADILK
                     EDQAQNTCIFSTEFALRMMGDVQQYFIDERVRNFYSVSISGYHIAEAGANPISQLAFT
                     LANGFTIVEYYLARGMKVDDFAPNLSFFFSNGMDPEYAVIGRVARRIWARALRDLYRA
                     GPRSQMLKYHIQTSGRSLHAREISFNDIRTTLQALYALFDNCNSLHTNAYDEALTTPT
                     EESVRRAVAIQLIINRELGLNKNQNPWQGSFAIEYLTDLVEEAVYKEFEAISERGGVL
                     GAMETMYQRGKIQEESLYYETRKHDGTLPIIGVNTYLSRSADEEGAHAELIRSTEEEK
                     QDQVSAVRAFQARNASAAPEALRKLQESASRGGNVFTELMQSVKSCSLGQISHALYDV
                     GGQYRRNM"
     gene            13560..14342
                     /locus_tag="VFB37_14345"
     CDS             13560..14342
                     /locus_tag="VFB37_14345"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO23680.1"
                     /translation="MRTRRTRPPQTGKRRPAPALNGHPASPWRASRERLLDRKVKREA
                     VIRAAAHAFNRKGYHNTSLDDIAAALDVTKPTVYYYVSNKEQLLFECFVAGVEQIRAA
                     FREVRSLRVPARERLSAVLRNYARAVASEFGWCMVRVEDQDLSAAMSAHIKAMKSEID
                     HGIRRLVREGVQDGSIHPCDPKMTAFALAGSLNWIAHWYRENQSMTAAQIADAFMAVF
                     DNGLRPRNGAGAGNGARRSASAGRTARARSARSRLPQAPLAR"
     gene            14366..15550
                     /locus_tag="VFB37_14350"
     CDS             14366..15550
                     /locus_tag="VFB37_14350"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZO23681.1"
                     /translation="MSEHEVVIVSARRTPIGAFLGALAPLSAPQLGAAAARAAIESAG
                     IQPKDVQEVIMGCVLSAGLGQAPARQAALAAGVPAGTPATTINKMCGSGLKAVMMAAD
                     QIRAGEAEIVLAGGLESMSNAPYLLPKARSGYRMGHGEVLDHMFYDGLQSPFDGKAMG
                     CFADATATRYNFTRAAQDAFAAESVRRAMKSLESGEFDAEVTPVTVKTRKGEVVVGRD
                     ETPGTCDISRISALKPAFGKEGTVTAASSSSISDGAAALIVMSAAAARARALKPLARV
                     LAYASHAHEPEWFTTAPVGAIRKVLSRLGWRPHDADLYEINEAFAAVTMAAMHEVDIE
                     HARVNINGGACALGHPIGATGARILTTLIHSLHRRGLKRGIASLCIGGGEAVAMAVEV
                     MP"
     gene            15740..16507
                     /locus_tag="VFB37_14355"
     CDS             15740..16507
                     /locus_tag="VFB37_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO23682.1"
                     /translation="MKIRDARAVVTGGASGLGAAVARHVIAQGGQVVMLDVQEGPGQS
                     MAAELGSRAAFLRCDVTSEAEVNSTMEAARGRMGGINLLVNCAGVVGAGRVLGKNGPM
                     AGDFFAKVVHINLIGAFLCDKAAAAIMQANTPAADGERGVLIHTASVAAFEGQIGQAA
                     YSATKAGLVGMTLPIARELAKFGIRCVSIAPGIFHTPMVDSMTPELQASLASQIPFPS
                     RLGQPEEFAQLVASIFEIPMLNGSTIRLDGAIRMQPK"
     gene            16573..18189
                     /locus_tag="VFB37_14360"
     CDS             16573..18189
                     /locus_tag="VFB37_14360"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HZO23683.1"
                     /translation="MNTAAVERDVMEYDVAIVGGGPAGLSCAIRLKQLKSDINICVLE
                     KASAVGAHALSGAVMEPGPLDSLLPQWRESPPGICVPAKRDEFRLLTPKRAFALPLPP
                     QLHNHGNFIISLGLLTPWLAQQAEALGVDVFAGFAAADVLFNTDQSVAGVRLGDMGVE
                     KNGSPGPNYTPGAEIRARTTVIAEGARGSLAKQLIRRFELDAHSCPQSFGLGMKELWQ
                     VPAGRVEPGLIQHSLGWPLDSATYGGSFIYHLDKDRLYVGFVVGLDYEDPRFAPFEAF
                     QQFKHHPTVKPLLEGGEILAAGARTIAAGGWQSIPRLEMPGAMLIGDSGGGVNLPKIK
                     GIHQAIRSGMLAAQHLVETGSPAGFDARWRASPGGQELRAVRNFKPGFKRGMWVGMAN
                     SALEVLTRGRTPWTLKNHLQDYQRLKQLSTYESPDRHWVERTLPPRDRLASVFFASTT
                     HDENQPAHLKVADTSICSERCTREFGNPCERFCPANVYEMVSDGKGGKRLQINAANCV
                     HCKACDIKDPYAIITWTTPEGGSGPNYQSL"
     gene            18199..>18812
                     /locus_tag="VFB37_14365"
     CDS             18199..>18812
                     /locus_tag="VFB37_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO23684.1"
                     /translation="MSEIWRPTPSRVADANLTHFMRCVSARRALAIGSHDELYRWSIE
                     EPADFWEELARFTAVQADWGSGPTLEYPDRMPGARFFPQARLNFAQNLLRFRDEQPAL
                     IFRNEAGARRRLSYRELQAEVARIADGLKGAGILRGDRIAGFLPNLPEAVIAMLATAS
                     LGAVWSSCSAEFGVPGVLDRFGQIAPKVIFAADGYLYAGKRIDCL"
BASE COUNT         3260 a         6004 c         6105 g         3443 t
ORIGIN      
        1 cggactgctg ctgggaggtc aagccgatat tgaagcgctg gttgtagaaa gtcaccacag
       61 ttgccagatc cttcgcagag ccgttgtgga agtacggcgc acgtgcagca acgccacgaa
      121 gtatcggccc cttggtcttg ccgatgtcct tccaattgcc ggtgatcaac gcccggcctg
      181 gatcagtcac ctggacagta gcacctgtgc tcgagttggt gagcgtatag accgggagat
      241 tggccaaatc cagtgcctga ttggcgctgc cattgttgtt ggtcggatgg gccatggtga
      301 ggccgatgtt gatcggcagg gatgtcgaat ggttgccgac gttcggattg ttgtgacata
      361 gaccgcaccc ggccaccacg tcctgacccg ccaggggatc ggcggcattg tagagcggat
      421 cggacggaat gctgttgatt cccggcacgt catggacgac gaacgtggtg ttgttgaaga
      481 tcgcctcgcc gcgaccgatg gcctgctgga ccggcgagag gtggcgatat tcgggactgc
      541 cgggctccca agcggagtag atcgtgaaca ccgtgttggt gaacggtgga ttcagcgggt
      601 cattgacgcc aatggagaag gccggcgtga cggtgttctg caggtagtag gcacccccat
      661 tggcgcccac actcagcagc gcgaccccgg tttcgggcac gagaacgacc tgcgccgttg
      721 acagtccttt ctcgaacgag acgatatcct gctgcgccgg catacccgca attggagcgc
      781 cctgcgcgtg accgatcgtt gccgagttcg cctgatcatc cagatcgaac agcagcgcag
      841 ccgaactacc attgtcacct gtctgggtcg tgatcttctg cagtgtttcg cgcccatccc
      901 acatcatcga tgacagcagc aggaggttgg tggagggcag cggacgcctg aaaagaaaca
      961 gatttcccga gatggccgct gaccccggcg ctatggcaca acccgcgggg ttcgtggctg
     1021 caaccaggga gaaatccgcg gtagcgggaa tcggcagttg gacgcggatc accgcgtatc
     1081 tgaggagcat cgatgagttc gcgcgattcg gggcttgatt accggaggtt gctggacagt
     1141 ccgagccatc gacgggagcg aacaatgaat cattgtgggc cagttgctgc gcatggcgcg
     1201 gtgtcagcgc ccatgcatcg gcctcgacgt ggcacgtggc acaagtgcgg ccattggttc
     1261 cgagccgagt aaagaaccca ttggtaaccc cggtatggaa gccgttcgtc gaggtgctct
     1321 ccagcacacc ggctgggtcc agggtcagcg gaaaccccgg gcctgagtcc tgcgcggagg
     1381 cgttgacact cgccgcggca aagcctgcga gcgatgcgac ccgaagaatc ctgcgagcga
     1441 gaggcgccgc tagctctgaa aatcgattca acaataattc catctgtgca acttctcttc
     1501 gctggcccct tactgagata tgcgtaaagg gaggcggaaa gacgacaaag agaggccggt
     1561 ctcttccaag ggaggccggg cggggactca cgggggcgcc ttgacggtcc atcgctcttg
     1621 gggaacgcat cggcttggct cgcgagcggt gaggcggcag actgtgcgcc gcgccgcggc
     1681 tcgccggtgc cctcaagggg gaattccatg ctcgtcttcg ccacgttcct gcgctcgtgc
     1741 attgcagcgc cggccgccgt catcatgagc ctcgtcctgc tttgcagcgc cgcgccggcg
     1801 atgggggcag agcaggccgc gccgatccgc ctctataccc tcgacggcgg cacgatcgaa
     1861 ttcaaagatc tcgcctcctt ctcggatacc ggagactacg acggcaagcc cggcacggtg
     1921 gccgtgccct gcttcctgat ccgccacccc aaaggcaccc tcatgtggga tacggggctg
     1981 tctgctgcgt ttgcaaagga cgatccctgg aagaagtacg gagtgcatgc gacggtcggc
     2041 gcctcgatcg agcagcagct caagcagatc gggctcactc cggcggacat cacctatgtc
     2101 gccttctcgc acctgcacat ggatcacatc ggcaatgcca acctgttcgc cggctcgacc
     2161 tggatcctga acaagaacga ggtggcctgg gcgagccgcg cggatgctcc ggcgggcgac
     2221 ctgagcacct tcagcgccta taagggcgcc aagacgaaga tgatagaggg agactacgat
     2281 gtcttcggcg acgggaccgt cgagattctg aaaaccccgg ggcatacccc cggacaccag
     2341 gtcctcctcc tccggttgaa atcaggcaac gtgctgctgt cgggcgatct gtggcacctg
     2401 cgcagcgaca tagcgggcca tatcgtctac agcggaaatt tcgatcgcgc gcaaaccctt
     2461 gcatccttcg atcgcgtcga gaagattctc aagaacagga atgcccggct gatcgtgcag
     2521 cacgatccgc aggactatca ggggttgccc aagatccccg cctttctcga gtagagtcgc
     2581 cgcgagtaga ggcgcttgga gtagcgccgt cccggacgca actcggtcgc tgtcacaaac
     2641 ctggaccccc ctccttcggg tacgccggca gcgccggcgg tggcggcggc agtgtggccg
     2701 gatgcttctg catctccccg aggatgtaat ggatggccgc ttcgagctgc acgtcgtgtc
     2761 cctgcatcca gtcggccggt gagtcctcga ccgtcatgtc cggatcaacc ccgtgattct
     2821 cgatcgccca ctgtgagttg agcaggtact gcgcgctctc cggcaccgtg atgtatccgc
     2881 cgtcgagcaa agtccactcg ccgcggatcc cacgcacgcc gccccaggaa cgggtgccga
     2941 tgagcggccc aagtccgtac tggcggaaga agaacgggaa gatgtccccg tccgaagccg
     3001 aataatgatt gagcagacag accttcggac cgatgatgag ttggttcggg atcgggatgg
     3061 cggcgtgctc ccgattggtg tccatcccga tgaggatgcg ccgcagccgc tcgagcacga
     3121 tctgatcgat gaatccgccg ccgttccagc ggtcatcgaa gatcagggcc tgcttgtcga
     3181 gctgcgcata gaactggcgg atgaactgat ccatgccgcg cccgcccatg tccgataagt
     3241 agatgtaggc gatgcggcct cccgaggcct ggtccaccac ttcgcggttg tgatcgaccc
     3301 aggcctgctc gcgcaggtcc acttcgttct tgaccggctg caccacgatg tcgcgccgct
     3361 tgccgccggg ggagtcggcg acggtcagct tcacggtcga atcctgcttg cctaccagca
     3421 ggctgaaggg atccatcggg gctttcagct cggtgccgtc gatggccaac aaataatccc
     3481 cggcgtgcac atcgagaccc ggcgccgtga ggggtgagcg ataagcatcg tgcgtattgt
     3541 caccgggcag gatcgtggcc agacgatagc gcccggatga gtcctccagc gcaaagtccg
     3601 cccccagaaa agcggtcgga acacgccgct cttccggcat gtcatctccg ccacccacat
     3661 aggtgtgcga gttgctgagc tcgccgatca cctcgccgat cagataattc aggtcgctcc
     3721 gtgagccgac gagcggcagc agcttgccgt aggattcgtg cacggccggc cagttcaccc
     3781 cgttcatctg cgggctgtag aacaagtcgc gttccaggcg ccaggccgag ttgaacatct
     3841 cggcccactc ccgcgtcggc tcgacccgca ccttcatgtg cgacaaatcg agtttcttct
     3901 tggcgtcctc gtccttgtcc ttgcccgggg ccgcatcggc gatgtagtaa tccttgtcga
     3961 gcttgtacaa caccttcttg ccatcatccg agagcgcgta cgcgctcagt ccgtccacga
     4021 tgttttcatc cttgcgcttc ttgagatcgt agacgtgcag gaccggcttc tcgccgggaa
     4081 gcggcccctc gatcatgggg ctgggtgtgg tgagatagaa aaccttgtca tcccgcacat
     4141 cgaagctggc gatgtccgcg ggcgtgatcg gcatggggac cgcccgggtc atcaacccgt
     4201 ccaactcgat ctccacggat gcagctgacg gtcgcttctt gcccgaggat ttcttgtcct
     4261 tttgatcctc cgaagattcc ttctgaccct cggcagactc cttgtgctcg tccgaggacg
     4321 cctcgtgatc ctccttgggc acgccttcgt cggaccgcgg cgcaaacagt gacgctgtcc
     4381 cgcgcttgag tgtggccaca tagatgccgg tcatcttcag ggtcgcgatg ttgaattccg
     4441 tgcggctgaa cgtcggattc tcgtgacgcg tggagatgaa gtacaggtac ttgccgtccg
     4501 gggcgaatgc cggctggaag tcgttgaagc gcgggtcgct gatcaatgtg gctttgcgct
     4561 tctcgaggtt atacagccag atccccgatt gttgattctc accggtgatg ctgtatgcga
     4621 gccagcgacc atcgggtgac caggtgtagt catgtatctc gttgaacttg tcctgcgcga
     4681 cctgtacggg agccccgccg tcgctgccga cgaaccacag acgatgctcg tgatcggaga
     4741 atgccagatg atcgcccttc ggcgaccagc gcggccggta gaaatagccg cgctcgaaat
     4801 gggtgaggac ccgctcggcg ccgccctcgg cgggacgaac ggcaatctgc tgctcaccgg
     4861 tgacatcggt ggtgtaggca atgctcttac cgtcaggaga ccaggcgggg tgatcctcgt
     4921 ccgcccctga ggtctgagtg agatttcgtg tgttaccgtg ctctgccgga agcgtgtaga
     4981 gatcgccgcg cgcggagaac accgcccggc cgccattcgg agccagatcg aagtccgtct
     5041 gctgcgccgt gtcctggtcg cggatggtgc tcttcgcatc cacccagtgt ggcccggtgc
     5101 gcgtgccgtc atccggcacc gtgatctcca gatcgtgaag ctgctcggaa ggcaggtcga
     5161 tcacatggag cctgcctccc tgctggaaca cgatgccggc ttcgccctgc gggcccgtac
     5221 ccaggctggg aaaatcgatg tcgtaatcgg tgaaatgcgt gatctgccgg aactgaccat
     5281 tggaagtgtc gtacgcccag atgttgcgtc gctcggcctg gtcgtggtcg gcaaggaaat
     5341 agatgatctt gccgaaccac atcggcgagg tctcggtgcc ggaccagtcg gtcacatgct
     5401 tgagctcatg agtgcggaaa tcgtagatgt cgatattctg gttgaggccg ccgtggtagc
     5461 gcttccaggt tcggaagtcg ctcaagatgc gcacataggc gatctgcgtg ccacccggtc
     5521 catagctcat gaaaccgccg cgatcgagcg gcagggccgt cggcagtccg ccgctcaggg
     5581 ggaccgagta gagcttgccg aaccaggtgt tccaggattg gcgccgcgac aggaagacga
     5641 tgccgctcga gtcgggcttc caggtgacca ccatgttgtt ggggccccag cgcagcggtg
     5701 cttcttcggt cacgtccgat gtgaaagtca ggcggcgagc ggggccgccg gctgagggca
     5761 tcacgtaaac gtcccggttg ccctgatact cgccggtgaa cgccagccag cgcccgtccg
     5821 gggaaaagcg tggcatgaga tcccgcccct cgtcagcggt cagccgcgat gcgacccccc
     5881 cggtgcgcgg tgctgcccag aggttatcgt gcgcaacgaa aacgatcgtc tggccataca
     5941 acgtcggata gcgcatcagc gtgtggccgc tggactgcgc atcggccagg cccgcgaggc
     6001 cggccatgac caggcccagc acgagccgct cgaaaagtag cgtggtgaaa ggaagtctca
     6061 tctggcaccc ctgacggaca ggaaaggcgc ggcgcaccgt ggggtgcgcg cctgcgctcc
     6121 ggggcgcgca tcttaacaac tgcgcagggg gctccccgca cgtttgggcg cagcagagca
     6181 tcgtttcgtc cgtgatatgc tcgagccaaa ccatgaactt ctgcaatcag tgcggcgcga
     6241 aggtcgagtt tcgcgttccg cccggcgatc acctgccccg ctacgtctgc ccagcctgcg
     6301 gggtcatcca ctacctgaat ccccgcctgg tggtcggctg cgttccggac cacgagggca
     6361 aaatcctgct gtgtcgccgc gccatcgagc cgcgccgggg gttctggacc gtaccggccg
     6421 gattcatgga gaacggcgag acgctgcagg aggcggctgc ccgcgagtcc tatgaggaag
     6481 ctcttgcccg ggtggagata ggctccctgc tgtccgtcgt gcatgtgctg catgcgcacc
     6541 aggtccacgt tttctttcgg gcgagactga ccgagccaac cttcggcgtg ggcgcggaga
     6601 gtctggaaac gctcctgctc gagccagaag cgatcccgtg gagcgaaatc gccttcccga
     6661 gcaccgattt cacgctacgg cactacctgg aagaccgggc tgcgggccgg gaaggccact
     6721 atttcacgca gttcgaccgc cggctgccgc gccagggcta gcccgtgcgt gcggcgcccg
     6781 tttgcgtcag gaccgccaaa ccggcgaaaa tccgcgtgag ggccgctgcg gatgagtgag
     6841 gataccgaat gacctttgtt gtgaccgaga actgcatcaa gtgcaaatac atggactgtg
     6901 tcgaggtctg cccggtggac tgtttccacg aggggccgaa tttcctcacg atcgatccgg
     6961 acgagtgcat cgactgcacc ctgtgtgagc ccgagtgccc gatcgaggcg atcttctccg
     7021 aggaggagct gccgcagggt caggagcact tcaagcaact caatgccgag ctggccaagg
     7081 tctggccggt gatcacggag aagaaggagc cgccggccga tgccaagtcc tgggaaggca
     7141 agccggacaa gctgaagctg ctcgagaagt agccgcccgg gcggccgtca gcgctttggg
     7201 cgcgatcagc cggcgcccgc actgcagggc ggtcgacgct tgtggatgct cagcgcttcg
     7261 gggcttcccg cagatgcgcc gcgagagtgt ccggcgggac gtacccatcg aacatttcgc
     7321 catccgcggt gacgatcgca ggagttccct tcagatcgaa cgttcgcccg agcgcgtatt
     7381 cccgggccac cggattaatc gcgcacggtg gtgtagtgag cgccattcca cgcttggcgg
     7441 cagtgagggc gtcgttgcgg ttgcgcgagc accagacttc ttcggccttc gtccaggatt
     7501 cagtgttggg cccggtgcgc ggataagaga gatagcgtac gcggatcccc aaccggttgt
     7561 agtcggcgat gtgcagatgc aactcgcggc aataggcgca atcgacatcg gtgaagacgg
     7621 tgacggtgta cttcggatcg cgcggcccga agacgatcat gtcgctctcc ggaatggctt
     7681 cgatgagctt ttgccgggcg gtacgccggc gctcctcggt gagattgtcg tttgcattga
     7741 gatcgatcag atccccgctg aaggcgtact tgccgtccgc cgtcacgtag gcgaattcca
     7801 tgccgcggga gacttcgtag atcccggtga cggctgaagg gtgcacatcg tccggatgcg
     7861 tcccgggaat cttgctggcg atctgtgcgc gcggatcctc ttgggctgcc ggttgcgcat
     7921 gcagcgcagg agccaacagg gtgaggatca gcagaaggct gggtctggcc ggcatggcgc
     7981 cgcagtctac atcagccccg ggggtgatgc tgggagtgca actctttgag gcgctcgcga
     8041 gccacgtgcg tgtagatctg ggtcgtggac agatcgctat gacccagcaa catctgcacg
     8101 acgcgcagat ctgcgccatg gttgagcaga tgggtggcaa aagcatggcg cagcgtgtgc
     8161 ggcgacagct ccttggagat gccggacttg cgcgcgtaac gcttgatgat gtgccagaag
     8221 gcctgacggg tcatgcgatc gccgcgacgc gtcgggaaca gataatcggt ctgccgctcg
     8281 agcaggatct cgctgcgcgc actgcttgcg aactcggtga gccagcgcat cgcctcctct
     8341 cccagcggga tgaggcgctc gcggttgcct ttgccaagga tgcggatgac accctggttg
     8401 gtgttgacct ggccatggcg caggttgacg agctcggaga cgcgcagtcc ggtggcgtac
     8461 agaacctcga gcatcgcccg atcacggtgt ccgaggggat cgctcaccac cggcgcattc
     8521 aaaagcgatt cgacctcctc ctcggtcagg gacttcggaa gcgagcggcc gatcttcggc
     8581 attgcgatct gcgccgtggg atcttcgcga atgacgcctt cgcgcaccag atagcggaag
     8641 aagcgtcgga agctggagag ctgccgcgca gtcgagcgcg gccgggcgcc ggcgttgaca
     8701 cgccaggcaa tgaagtcctg caggtctgcg cgcgtggtgc gcatcatcgg cacatcgcgc
     8761 tcggcgagcc agcgggacag ggccgtcaga tcggcgcggt acgccgccaa ggtattcggc
     8821 gacagtcctc gttccatcca gacggcatcg agaaaccggg acactgccgg gtcggcagag
     8881 tcggcatggg cagtcatggc gatcgcggca gcagggttcg acaagattcc ctctcgctgt
     8941 aagcctgcgc gcgccgcccg gcgcgccttg aggacaggcc tcggtgtatg agcttcgaca
     9001 atccagtgtc gctgccgcag tatggtgagg ccccgcggca acctgcgtga ttgccttcac
     9061 atctactgcc aagcattaac ataaagagaa ccaggtcaca tctcgatcac ccgggcaacg
     9121 ctatacgcac catgccattc caaggctctc acggacttgt gaccgcagca cttcggatcc
     9181 tgctcgcccc ggtgcgaatc gcttacggcc tgtacgtcct cgccgtgttc ttcgtcaccg
     9241 gctcctgcgc tttcgctctg acggtgatcc ttccggggct cgagcggcgt cgcgccgtcg
     9301 cccggttctc ctcactcgca ttcctgtggc tcgccggcat gcccctgacg gtgagatctg
     9361 ccagccatct gccccggggt caatgtgtcg tcgtttccaa ccacgccagc tatctcgacg
     9421 gggtggtctt cacggcggca ctcccgccgc gattcggatt cgtcatcaag cgcgagatgg
     9481 ctggcgtgcc gctcgcgggg ctgctgttgc ggcgattggg gtcggagttc gtcgagcgct
     9541 tcaatcgcca tcagggcgcg gcggacgccc gacgaatgct acgacgcgcc gtggaaggga
     9601 attcgctggt gttctttccc gaagggacgt ttacccgtac gccgggtctg ctcaaattcc
     9661 ataccggcgc gttcgtcaca gcggctcgag cgggctgtcc ggtcgtgcct gccatcgtgc
     9721 gcggttcccg gcgagcactc tcgcctcagg gcaagttgcc ccgccccggt cggatcatcg
     9781 tggagatcct gcctgcggtc gcgctgacga cgaaagaccc cgcgacgctg cctatggatc
     9841 tgcgtgagcg ctcgcgggag gcgattttgc gggcgctggg ggagcccgat ctcgcaggct
     9901 cgcatcccca cgatcccgct cctgaacgcc ccgctcagtc gctagaacag gacgcctgag
     9961 gcgtaaccgc gcgagccgac aaggactcgc gcgttacatg ttacgacggt actggccgcc
    10021 tacgtcatag agcgcgtgcg aaatctgccc cagcgaacag ctcttcaccg actgcatcag
    10081 ctcggtgaac acattgccgc cgcgcgaggc gctctcctga agctttcgca gcgcctccgg
    10141 cgccgcgctc gcgttgcgcg cctggaaagc ccgcaccgcg ctgacctgat cctgcttctc
    10201 ttcctcggtg gagcggatca gttccgcatg cgcgccttct tcatccgcgg aacgtgaaag
    10261 ataggtgttc acgccgatga tgggcagcgt cccatcgtgc ttgcgggtct cgtagtacag
    10321 cgactcttcc tggatcttgc ctcgctggta catcgtctcc atggccccga gaaccccgcc
    10381 tcgctcggag atggcttcga actccttata gaccgcctct tccacgaggt cggtcagata
    10441 ctcgatggcg aaggagccct gccagggatt ctggtttttg ttcaagccga gctcgcgatt
    10501 gatgatgagc tgaatcgcaa ccgccctgcg cacgctctcc tcggttggcg tggtgagcgc
    10561 ctcgtcgtag gcgttggtat gcagactgtt gcagttgtcg aacagcgcat acagcgcctg
    10621 cagcgtcgtg cggatatcgt tgaaggagat ttcccgcgca tgcagactac ggcccgaagt
    10681 ctgaatatgg tacttgagca tctggctgcg cggaccggcc cggtacagat cgcgcagcgc
    10741 tcgcgcccag atgcgccgcg ccaccctgcc gatgaccgcg tattccggat ccatgccgtt
    10801 ggagaagaaa aacgacaggt tgggcgcgaa gtcatcgacc ttcatgccgc gcgccaggta
    10861 atactcgacg atggtgaagc cgttcgcgag cgtgaaggcc agctgcgaga tggggttcgc
    10921 cccggcctcg gcgatgtgat agccggagat ggacacggaa tagaaattgc gcacccgctc
    10981 atcgatgaag tactgctgca cgtcgcccat catgcgcagg gcgaactcgg tggagaagat
    11041 gcaggtgttc tgcgcctgat cctccttgag aatgtcagcc tgcaccgtgc cgcgcaccgc
    11101 ctgcagggca tccgccttga tccgctcgta agtctcgcgc tcgaggagtt gatcgcccgt
    11161 caccccgagc agtgccaggc cactgccatc gtggcccttg ggcagctcgc ccttgtaggc
    11221 gggcggcgcg atgccgccag ccgtgtgcgc gccgatgagc gcagtgatcc gcgcctgtgc
    11281 cgcctcccag cgcccgtcgg ctctcaggta gcgctcgacc tgctgatcga tggccgtgtt
    11341 catgaacatg gcgagaatca tcggcgccgg accattgatg gtcatcgaca cggaagtgtt
    11401 cgagcggcac agatcgaagc ccgaatagag cttcttcatg tcatcgagcg tcgcgatcga
    11461 gacacccgaa ttacccgtac ggccatagat gtcgggccgg gtgtccggat cctcgccgta
    11521 cagcgtggtc gagtcgaaag cggtggagag ccgcgccgcg ttatgaccgc cggcgagata
    11581 gtggaaacgg cggttggtac gttccggcgg gccttcgccg gcaaacatcc gggtcggatc
    11641 ctcctgctcg cgccgatacg gatacacccc accagtgtag gggaacgagc ccggcaggtt
    11701 ctcggtcgcg acaaatcgca ggatctcgcc ccagtcgcga aactttggca ccgctatctt
    11761 ggggatgcgc tggtgagaga ggctctcggt gtagttctcg ccgctgaccg tcgcattgcg
    11821 gaccttgtag gcatatgtcg cgtcggttgc ggcgcggacg cgttccggcc atgccttgag
    11881 ctcgccgatc ccctccgctc cgatctcatc cagggaacgg ttgtaggtgc ggcgcagctg
    11941 ctgccgggtc gcatccgcgt gctccagcaa cgccgagtcg gcaaaccgct cgagcggtgc
    12001 aggcagcgct ggatcctgca gcgcctgcag ggcctcgtag attccgcttg cgcgacgagc
    12061 cgcctcggcc tgctcctcgg cgcgctggcg cgaagtgcga ccgtgttgtg caatctccgc
    12121 caggtagcgg atgcgctggc ccgggatcag gggctcgcgc ttcgtgagct cggtcggtcc
    12181 gacatcagtc acctgccagg actcggcacc gactttcttt tcatccagtg cccggcacag
    12241 tgccgcgaac agccggttca ccccaggatc gttgaagcgg ctggcgatgg tcgaatacac
    12301 cggcacttcg gcatcggcga tccgcgcctg ctcgggatgg ttgcgccgcc actgcttgcg
    12361 tacatcgcgc aggctgtcct ccgcgccgcg cttctcactc ttgttcagga cgatgaagtt
    12421 cgcaaagtcg atcatgtcga tcttttcgag ctgactcgcc gccccgtact cgctggtcat
    12481 gacatagagc gacagatcga cgagatccac gatctcggtg tccgactgac cgatgccggc
    12541 tgtctcgacc acgatgagat cgaagcctgc ggccttgtag agctcgatga cctcccgcag
    12601 gactgcggcg gtcgccagat gctgccgtcg ggttgcgagc gagcgcatga agatcgactc
    12661 gctggcaagg ctgttcatgc ggatccgatc ccccaggagc gccccgccac tgcgccgccg
    12721 tgtcggatcc atggcgacca tggcgatctg ccggccgggg aactggcgta cgaagcgcgt
    12781 cagcagctca tcgctcaggc tcgacttgcc ggcgcccccc gtaccggtga tgccgatgac
    12841 cggcacctgg tggcgcgagc gggcaagcgc gcgcctgatc tgggcggcct gaggatccgc
    12901 ctccccggcg cgctcgagta tggagatcgt ccgggcgatg tggcgctgat cgcgcgcgtt
    12961 cagcggcccg aactcgtacg ggggcttggt cacgctcttc aggcgacggt agacgtcatc
    13021 aatcatgccg ttgagcccga gcctgcggcc gtcttcgggg gtgtagatct tttccacccc
    13081 gtgccgctcc agcacttcga tctcgtgcgg cgcgatcgtg cccccaccgc cgacgatgac
    13141 gcgaatgtgc tcgcagtcac gctcgcgcag catgttcacc atgtactcga agtactcgtt
    13201 gtggccgccc tggtaggagc tcaccgcgac ggcatcggca tcctcctgta tcgccgcccg
    13261 cacgatgtca gcgacgctgc ggttgtgtcc caggtggacc acttccgcgc cgtgtgcctg
    13321 cagcaggcgg cgtatcatat tgatggccgc atcgtggccg tcgaacaggg aagcggcgga
    13381 gacgaaccgc aaaggcggct gcgcggccga ttgctcgggc cgcagcagcg ccgtaccgcc
    13441 ttcggtactg gcattcatgg ggtttacgca ccggtaggat gactaccggc cagtatgatc
    13501 ctataatgag ccgtccttca agcacccgct gagccgtcct cgagcaccgc tccacccgca
    13561 tgagaactcg tcgaacacgc ccgccgcaga ccggcaagcg ccggccggcg ccggccctca
    13621 acgggcaccc ggcctcaccc tggcgcgcga gtcgggagcg gctgctggat cgcaaggtga
    13681 aacgcgaggc ggtgatccgc gcggccgccc acgccttcaa ccgcaagggt tatcacaaca
    13741 cctcgctgga cgatatcgcg gccgctctcg atgtcaccaa gccgacggtg tactactacg
    13801 tcagcaacaa ggagcagctg ctcttcgagt gcttcgtcgc cggcgtcgag cagatccgcg
    13861 cggcgtttcg cgaggtgcgt agcctgcggg tcccggcccg cgagcgcctc agcgccgtgc
    13921 tgcgcaacta tgcccgggcc gtagcctccg agttcggctg gtgcatggtc cgtgtggagg
    13981 atcaggacct gtcggctgcg atgagcgcgc acatcaaggc gatgaaatcc gagatcgacc
    14041 atggcatccg ccgcctggtc cgcgaaggcg tgcaggacgg ctcgattcat ccctgcgatc
    14101 cgaagatgac cgcctttgcg ctcgctggct cgctcaactg gatcgcccac tggtaccgcg
    14161 agaaccagtc gatgaccgct gcgcagatag ccgatgcgtt catggccgtt ttcgacaacg
    14221 gactgcggcc acgcaacggc gcaggcgccg gcaatggtgc gcgccgaagc gcaagtgccg
    14281 gccgcacggc ccgggcgaga agcgccaggt cgcgcctgcc ccaagcgccg ttggcgcgct
    14341 gagaattccc tggagggctc ccaacatgtc agagcatgaa gtcgtcatcg tctcggcccg
    14401 gcgcaccccg atcggcgcct ttctgggagc gctcgcgccg ctgagcgcgc cgcagctcgg
    14461 cgccgcggct gcccgtgcag ccatcgagtc ggccggaatc cagccgaagg acgtccagga
    14521 agtgatcatg gggtgcgtgc tgtcggccgg gctgggtcag gcgccggccc gccaggcggc
    14581 gctcgctgcc ggggtgcccg ctggcacgcc ggccaccacc atcaacaaga tgtgcggctc
    14641 ggggctgaag gcggtgatga tggccgcgga tcagatccgc gccggcgagg ccgagatcgt
    14701 gctggccggc gggctcgagt ccatgagcaa tgccccgtac ctgctgccca aggcgcgcag
    14761 cggctatcgc atgggtcacg gcgaggtcct cgatcacatg ttctacgacg gcctgcagag
    14821 cccgttcgac ggcaaggcca tgggatgttt cgcggatgcg acggccaccc gatacaactt
    14881 cacgcgagca gcccaggatg ccttcgcggc cgagtccgtg cggcgcgcga tgaagtcgct
    14941 cgagtccggg gaattcgacg ccgaggtgac tccggtgacc gtcaagaccc gcaagggcga
    15001 ggtggtcgtc ggacgcgatg agacgccggg cacctgtgac atcagccgga tctccgcgct
    15061 caagccggcc tttggcaagg agggcaccgt gaccgccgcg agctcctcct ccatctccga
    15121 cggcgcggcc gccctgattg tcatgagcgc cgccgcagcc cgcgcccgcg ctctgaagcc
    15181 gctggcgcgc gtgctcgcct atgcgagcca cgcgcatgag cctgagtggt tcacgacggc
    15241 gcccgtgggc gcgatccgta aggtgctctc cagactcggc tggcggccgc acgatgcgga
    15301 tctgtacgag atcaacgagg cattcgccgc ggtcaccatg gcggcgatgc acgaagtgga
    15361 catcgagcat gcgcgggtga atatcaacgg cggagcttgc gcgctgggac atccaatcgg
    15421 cgccaccggc gctcgcatcc tgaccacact gattcattcc ctgcaccggc gcggcctcaa
    15481 gcgtggaatc gcctccctgt gcatcggcgg tggtgaggcg gtagccatgg ccgttgaagt
    15541 gatgccctga gcctgagcct gcgggtgccg gttccgggcc tgctcgccgg gtgcgcagcg
    15601 ccgcccgcgg ggtgaggttt gggcaagccg gtgtcgcctc tgggcgggcg ccccggcgga
    15661 ccgattccca ggatgcaggg ggtatgctct actggcgcgc ccattctcca acccgaatca
    15721 acctcgacca ggaagctcca tgaagatccg agacgcgcgc gccgtggtga cagggggcgc
    15781 ctccggcctg ggcgccgcgg tggcccgaca tgtgattgca caaggcggcc aggtcgtcat
    15841 gctggatgtt caggagggcc ccggccagag catggcggcg gagttggggt cgcgggctgc
    15901 cttcctgcgc tgcgatgtca cctccgaagc cgaagtcaac tccaccatgg aagcggcccg
    15961 gggccggatg gggggtatca acctgctcgt gaactgtgcc ggcgtggtcg gcgcgggacg
    16021 agtgctcggc aagaacggcc cgatggccgg cgattttttc gcgaaggtcg tgcacatcaa
    16081 cctgattggc gccttcctgt gtgacaaagc cgccgcggcc atcatgcaag ccaacacacc
    16141 ggctgcggac ggtgagcgcg gtgtgctgat tcacacggcc tccgtggcgg ccttcgaagg
    16201 tcagatcggc caggccgcct actcggcgac gaaagcgggg ctggtcggca tgaccctgcc
    16261 gatcgcgcgc gaactggcga aattcggcat ccgctgcgtc tccatcgcgc cgggcatctt
    16321 tcatacgccg atggtggatt cgatgacccc ggagctgcag gcctcgctcg ccagccagat
    16381 cccctttccg agccgcctcg gccagccgga ggagttcgcg cagctcgtgg cctcgatctt
    16441 cgagattccc atgctgaacg gcagcacgat ccgcctcgat ggcgcgatcc ggatgcaacc
    16501 caagtagttc gtcatgagcc gcggcgcaca ccctgaagta cggcgcgctc ccgagcaatt
    16561 aagggcattg ccttgaacac cgcggccgtt gaacgggatg tgatggagta tgacgtggcg
    16621 atcgttggcg gcggacccgc aggcctgtcg tgcgccattc gcctgaagca gctcaagtct
    16681 gacatcaaca tctgtgtcct ggagaaggct tcggccgtgg gcgcgcacgc gctctcgggc
    16741 gcggtgatgg agccgggtcc tctggattcg ctgctgcccc agtggcgcga gtcccctccc
    16801 ggaatctgcg tgccggcgaa gcgcgatgag ttcaggctcc tgacacccaa gcgagccttt
    16861 gcgctgcccc tgccgccgca gcttcataac cacggcaact tcatcatctc gcttggactg
    16921 ctgacaccct ggctcgcgca gcaggccgag gcgctgggtg tggatgtgtt tgcagggttc
    16981 gccgctgcgg acgtgctgtt caacactgac cagtcggtag cgggtgtacg cctgggtgac
    17041 atgggagtcg agaagaacgg cagccccgga cccaactaca cgcccggcgc cgagatccgc
    17101 gcccgcacga cggtcatcgc cgagggtgca cgcggtagcc tcgccaagca gctcatccgt
    17161 cgcttcgagc tcgacgcgca cagctgcccc cagagcttcg gcctgggcat gaaggagctg
    17221 tggcaggtgc cggcgggccg cgtcgagccc ggcttgatcc agcactcgct cggctggccc
    17281 ctcgactccg caacctatgg cggtagcttc atctaccatc tcgacaagga tcgcctgtat
    17341 gtcggcttcg tcgtcgggct cgactacgag gatccgcgct tcgcaccgtt cgaggcattc
    17401 cagcaattca agcatcatcc gacggtgaag ccgctgctcg aaggggggga gatccttgcc
    17461 gcgggagccc gcaccatcgc cgccggcggc tggcagtcga tcccgcgcct ggagatgccc
    17521 ggtgcgatgc tgatcggcga ttcgggtggc ggggtcaatc tgcccaagat caagggaatc
    17581 catcaggcga tccgctccgg catgctcgcc gcgcagcatc tggtcgagac cggctcgccc
    17641 gccgggttcg atgcccgctg gcgcgcctcc cccggagggc aggagctgcg cgcggtgcgc
    17701 aatttcaagc cgggattcaa gcggggcatg tgggtcggca tggcaaactc ggcgctcgaa
    17761 gtcctcacgc gcggccgcac gccctggacg ctgaaaaatc acctgcagga ctatcagcgg
    17821 ctcaagcagc tctccactta tgaatcaccc gatcggcact gggtggaacg gacgctgccg
    17881 ccgcgcgacc ggctggcatc ggtattcttt gcctcgacca cccacgatga gaaccagccc
    17941 gcgcatctga aggttgcgga cacgagcatc tgctctgagc gctgcactcg cgagttcggc
    18001 aatccatgcg agcgtttctg cccggccaac gtgtatgaga tggtgtccga cgggaaaggc
    18061 ggcaagcgct tgcagatcaa tgccgccaac tgcgtacatt gcaaggcatg tgacatcaag
    18121 gacccgtacg cgatcatcac gtggacgact cccgaaggag gctcgggacc caactaccag
    18181 tctttatagc ctgagcgcat gtccgagatc tggcgcccaa ccccctcacg tgtggcggac
    18241 gcgaatctga cgcacttcat gcgctgcgtc agtgcgcgca gggcgctcgc gattggctct
    18301 cacgatgagc tgtatcgctg gtcaatcgag gaaccggccg acttctggga ggagctcgca
    18361 cgcttcaccg ccgtacaggc cgactggggc tcaggcccca ccctcgagta ccccgaccgc
    18421 atgccgggag cgcgcttctt cccgcaagca cgcctgaact tcgcgcagaa tctgctgcgc
    18481 ttccgcgacg agcagcctgc cctcatattc cgcaacgaag cgggcgcgcg gcgccggctt
    18541 tcgtatcgcg agctgcaggc cgaggtggcg agaattgccg acggattgaa gggcgcgggc
    18601 atcctcaggg gcgatcgcat cgccggcttc ctgccgaacc ttcccgaagc cgtgatcgcc
    18661 atgctcgcga ccgcgagcct gggcgcggtc tggtcctcgt gctccgcgga gttcggcgta
    18721 cccggcgtgc tcgatcgctt cggacagatc gcgccaaagg tgatcttcgc tgccgacggc
    18781 tatctctacg caggcaagag aatcgactgc ct
//
LOCUS       DASRHN010000359        23272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1454221, whole genome shotgun sequence.
ACCESSION   DASRHN010000359 DASRHN010000000
VERSION     DASRHN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23272
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454221"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..708
                     /gene="flgB"
                     /locus_tag="VFB37_14370"
     CDS             307..708
                     /gene="flgB"
                     /locus_tag="VFB37_14370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HZO23685.1"
                     /translation="MPLNLDTYLGVHADALKLQSRRMEVLANNLANVDTPNFKARDID
                     FRRALAAAGGADAPVKMAATDPRHLGADPASVQEADLKYRVPLAPALDGNTVDAQQEQ
                     AAFADNTVRYQATLTFLSTRFKNLLTAITGS"
     gene            731..1162
                     /gene="flgC"
                     /locus_tag="VFB37_14375"
     CDS             731..1162
                     /gene="flgC"
                     /locus_tag="VFB37_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HZO23686.1"
                     /translation="MSSFKIFDIVGSGLSAQSVRLNTVASNLANADSVSGDPNTVYKA
                     RHPVFQAVKAAVGQSADPIDRANGASVRVSGIVQSDAPPTERYDPGNPLADEHGNVYA
                     PNVNVIEEMTDMISASRAYQDNVEVMNTSKDLMLATLKLGD"
     gene            1183..1827
                     /locus_tag="VFB37_14380"
     CDS             1183..1827
                     /locus_tag="VFB37_14380"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZO23687.1"
                     /translation="MSTTNPITGITTGIATSTNSVPANMQIDQSDFLQLITTQLQNQN
                     PLQPADPTQFVSQLEGMSEVSSMQSMQSSLQASQVMSGTALLGHSVLAPGASANLTAG
                     GSISGAITAPAGASNLAVTISNSSGTPVSSFNVTPQATGLTQFSWNGHTAAGAAAAGM
                     YQIQVNATVNGTTQPVNPMVVSKVQSVTLDPSTQAVDLLTDSGTVALSSVVSIL"
     gene            1861..3081
                     /gene="flgE"
                     /locus_tag="VFB37_14385"
     CDS             1861..3081
                     /gene="flgE"
                     /locus_tag="VFB37_14385"
                     /inference="COORDINATES: protein motif:HMM:NF004238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HZO23688.1"
                     /translation="MSFNIALTGLDAANQDLSVTANNLANVATTGFKGSRAEFADLFA
                     STQQGVSATAVGNGVAVSEVAQQFTQGNIETTGNNLDLAISGNGFFTMSQNGALSYTR
                     DGEFQVDNSGNVVNAQGANLQVYPPLTTGGFNTGGLTNLSLTTGESAPQATANAQITA
                     NLPASASQPADAVFSPTDPASYNNSTSLTVYDSLGAAHTATLYFIKGATTNDWSSQLY
                     VDGTAVGTPQALDYSNTGALTTPANGQVTFPAYTPATGAAPLNMTFNFSNTTQFGDNF
                     GVTAVSQDGFTTGKLTGISIDSTGVVQARFTNGRSVNLGQVALANFANPQGLQQLGNA
                     SWAQTNASGAAVAGVAGNSGFGTIQSGSIEESNVDTTSALVDMIVAQRDFQANAQMIQ
                     TNDQITQTIINIRQ"
     gene            3111..3851
                     /gene="flgF"
                     /locus_tag="VFB37_14390"
     CDS             3111..3851
                     /gene="flgF"
                     /locus_tag="VFB37_14390"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HZO23689.1"
                     /translation="MDKLLYVAMSGAKDTLRAQAANSHNLANASTTGFKADLSAFQSR
                     AVAGPGYASRVYATDATVGWDHGVGAQTTTGNPLDVAIQGSGFIAVQDVTGQEAYTRA
                     GDLHVDPGGQLLTATGLPVMGDGGPISVPPSTSISVAADGTVSIVPLGQSPQTVANVA
                     RIKLVNPPSSTIERGSDGLFRTTDGQPATADASTTIASGVLESSNVDLASCMVNMIEL
                     SRRFDLQVKALHTAEDDGAASAKLLQSS"
     gene            3888..4673
                     /gene="flgG"
                     /locus_tag="VFB37_14395"
     CDS             3888..4673
                     /gene="flgG"
                     /locus_tag="VFB37_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HZO23690.1"
                     /translation="MEPALWAAKTGLDAQQTQMTVTANNLANVATNGFKKSRAVFDDL
                     LYQNVSQVGAATSQSTVSPTGLQLGTGVRVVSTERSYTQGNLDNTGNSLDLAIQGRGF
                     FQLLQPDGTLAYTRDGSFQVNAQGQLVNSSGYAVQPAITIPTGAQSVTIGSDGVVSVL
                     VAGQSAPTQVGSIQLADFINPAGLQTVGGNLAVESASSGPPQTGTPGLTGLGTLVQGS
                     VESSNVDVVEEMVNMIETQRAYEMNSKAVQTTDQMLQYATENM"
     gene            4678..5352
                     /locus_tag="VFB37_14400"
     CDS             4678..5352
                     /locus_tag="VFB37_14400"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HZO23691.1"
                     /translation="MMRFLSISALALGLLAALGCHTVKPPKDDDGMSWAQEPATPPTN
                     GAIFQTGREVALFENPIAHHVGDIVTIVLNEQTAAQKSATTTTTKNTSDTLPGMTLLG
                     KAATIHGTPILSNNINDATKFDGEGDSKQSNSLSGFITATVIRVLPNGNLFIKGEKWI
                     GINQGQEYVRVSGVIRPIDLAPDNSIPSQRVASAKISYGGKGALADANAQGWLSRFFN
                     SPWTPF"
     gene            5337..6488
                     /locus_tag="VFB37_14405"
     CDS             5337..6488
                     /locus_tag="VFB37_14405"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZO23692.1"
                     /translation="MDTVLIMPTLSAPRSALLCALIALAVLAAGTPPAQAERIKDIAS
                     VSGVRTNQLVGYGLVVGLDGSGDQTSQAPFTIQSIANMLAKFGVTMPSNSNPQLKNVA
                     AVTVTADLPPFSKTGQTIDVTVASIGNAASLRGGDLLMTPLRGVDGQVYAFAQGSVVV
                     SGFGVNGKDGSKISLNVPSSGRIPNGASVEREVPIKFAADPHLTLNLHTPDFTTAARV
                     AAGINKALGSGTADAVDAVSIRVSAPVEADQRIAYLATLEAIEIEPGEAPARVIINSR
                     TGTVVISSHVKVMAAAVAHGSLSVTITERSDVSQPNSFTNGTTATVTPRSTLDVQQAD
                     ARMFVFQSGVNLDEIVRAVNQVGAAPGDLVAILEALKQAGALRAELIVI"
     gene            6544..7500
                     /gene="flgJ"
                     /locus_tag="VFB37_14410"
     CDS             6544..7500
                     /gene="flgJ"
                     /locus_tag="VFB37_14410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HZO23693.1"
                     /translation="MSLVKDAALPSTAAPAYTDLAGLSALKQGAAGHDPSAIRRVAQE
                     FESLFTRMMLKSMRDAVGPDPMFGSDQEQMYQGMADDQLSVQLSRGRGLGLADMLMRQ
                     LQKLGVPGAAAAAQKSSTARAGSAGAAAYGAVQRAASASPPASSAQQASFIQGLWPEA
                     QQAGQALGVDPRNLIAQAALETNWGRNVPQGSGGATSNNLFGVKASDWKGPAVAATTQ
                     EYQGGAAVSTNAQFRAYASPAESFRDYVALLRDNPRYAAALNTGADVHAFAAGLQRGG
                     YATDPDYVRKMSATALNVTLAQNVSQLKSADAAPITGNTGTL"
     gene            7513..9420
                     /gene="flgK"
                     /locus_tag="VFB37_14415"
     CDS             7513..9420
                     /gene="flgK"
                     /locus_tag="VFB37_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZO23694.1"
                     /translation="MSDILSTSVSGLLAFQRALDVTSNNVSNAATPGYSVERVELAEN
                     PAQATASGYIGSGVNVQSVTRSYDELLAGQVRSSQSSYSSFNTFATQAAQIDNMLSDS
                     STGLTASLQNFVNALQTVANSPSSTAQRQVLLSQAQSLTQQMQNYDSQLSTYSAGVES
                     QITSGVSQINTLASGIANLNGQIASALAGTGQPPNDLMDQRDSLIDQLSQYVTVNTAT
                     QSDGSMNVYIGAGQALVTGSQAQTLTAIQDPYDASQHDIGIVSGGNTADVTSEISGGS
                     LGGLLAVRTQVIEPTQNTLGQFSVGLASIVNQAQQSGMDLTGSTGQPMFSVGGVQILP
                     ASTNQGTGAVSATRASLSGLTADDYKLTFSGGAWQLQDTASGQSVAMTGAGTNASPFQ
                     AAGLSIVVSGAATNGDSFLIRPTAAATAGLSVLLSNPAQIAAASSIQTTAATANIGTG
                     TVSGSAITDPSTWVPGTYTISFSSASQYQVTNSGGTVVASGTYTAGTPIAFGGAAVTL
                     TGAPAAGDTFQVGAASPSNTGDNSNAFAMIDALSASSLNGGTTSLTSVANNLVSQVGV
                     LTQQSQANASAQQSVNQSAVDTRNNLSGVNLDEEAAKMVQYQQAYQACAQLIQTAGTM
                     FNSLITAISNG"
     gene            9423..10616
                     /gene="flgL"
                     /locus_tag="VFB37_14420"
     CDS             9423..10616
                     /gene="flgL"
                     /locus_tag="VFB37_14420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZO23695.1"
                     /translation="MRISTVTFQQDALGQMETLQADLSKTQNQLGTGKKLNSAADDPA
                     GMAQVNQLNVELSASEQYVTNGNAASSNLQLEEQAMSDATNTLQSARDLAVQANNSSL
                     TAAQRTDIATQLSQQLQDLIAIGNRTDSNGDYLFSGEASGTTPFAQVGNAVTYSGSSQ
                     VRQVQISPNQRISAGDTGASVFMNVPAGNGTFTTAAGTTNTGVGAIGVGSVTNAAAWV
                     PDTYTVKFTSPSAYTVTNSSGTTVASGTNFTDGQSISFNGIQVAISGTPAGGDTFTIA
                     KAGNSSAFNTLQGLITTLNSPNLNNAQLATQIGGALQQIDNAISNLNDVSASAGARVN
                     SITSSQATAQTTQTNLSGAISSLSDVDYAAAITQLNTEELSLQAAQQSYASIAHLSLF
                     DYIHS"
     gene            10897..12093
                     /locus_tag="VFB37_14425"
     CDS             10897..12093
                     /locus_tag="VFB37_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZO23696.1"
                     /translation="MSLVLNTNYSSMVAQNSLTASGGQLATALQQLSSGLRINTAADD
                     AAGYAISQGMTSQINGINQASRNANDGVSLAQTASGALQEITNDLQTMRDLAVQSLNA
                     SNSATDRTDLDAQFQQLKADINSVASQTQFNGVNLLDGTFQGATFQVGANAGQSITMA
                     AISSAGSGNLGQLYGVAATDPATYAGGNTDTVSFDVTVNGTKYTTSTVTLTGTQSTDL
                     KAVAAAVNQAVGQQGVVASVDSTGKGINLSSTTSQTVTIATTATKGTGTGAAGGETAA
                     SFGMVEGSNAITTAGNFLSKGDVTTVDNSNLVLIQVDAALQQLATTGAQLGAYQNRFQ
                     AAITGLNTDSTNLSSARSQIQDTDYAAATSQLSKAQILQQASTAMVAQANTIPQNILT
                     LLQKLP"
     gene            12204..13682
                     /gene="fliD"
                     /locus_tag="VFB37_14430"
     CDS             12204..13682
                     /gene="fliD"
                     /locus_tag="VFB37_14430"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZO23697.1"
                     /translation="MASTITPTSNSTSTSAPVSIASNSTAGAAGGSVIDVSSLVSQLV
                     AATRAPQDALISNQTQAVTTQISAIGTLKGALSAFQSALGAIDTPSSFAAMTANTSDS
                     TIFSASAGSGAVAGTYPVTVSQLAQGQQLVSKGFAGGSSSTIGTGTLQLTLGSSSFSV
                     SIDSTDDTVEGLAAAINGAGANPGLTATVINGTDGAHLVLSSTLTGAANTIGVTETDG
                     GGALAAVTYGSGNTANYTQNAAAQDAAFSISGIDYTSSSNTVSNALDGVTLTLLGTTP
                     TSGTGGGAATPETLTVANDTPTIESNISAFVKAYNTLVTSVSALNSYDPTTGTAGPLL
                     GDALMSGVQNEIRSTLYSVVNTGSAVYNSLASVGITTNSDGSLSLNNSTLQSALATAP
                     TAVSALFSGTSGIASGLNTQLTGELSSGGVIDSRSQTLVQQENALSEQTNQLNTKMDA
                     LTASLTQQYSALNTLLSSLQSTSAYLSQQFAALPLVQGQPNA"
     gene            13731..14252
                     /gene="fliS"
                     /locus_tag="VFB37_14435"
     CDS             13731..14252
                     /gene="fliS"
                     /locus_tag="VFB37_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZO23698.1"
                     /translation="MTEQKAVSSYARNSKLAAYQSVSVHGGVAEADPHTMVLMLMDAA
                     LERMASARGCIERRESARQAGLLHSCVQIVGELRGSLNMADGGTLAQNLADLYEYMIR
                     RLLLANAASDVAPLIEVQRLLDEIRSAWVAIGPQVKQTANAPAAATWNASANTAAAPQ
                     TTASGVTPGNSTV"
     gene            14298..14681
                     /locus_tag="VFB37_14440"
     CDS             14298..14681
                     /locus_tag="VFB37_14440"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HZO23699.1"
                     /translation="MAEEIDAIARVGAVGSTDLAPPPDSGAGTSSAARTTAAAGQPAD
                     KLPSQPSAAAIQAAVQQVNAHLATVNRVLELHVDAGTGLTIATIRNAQTGAMLQQFPS
                     ADSIHLAQMLADWSPGKHVLVDLIA"
     gene            complement(14885..18802)
                     /gene="purL"
                     /locus_tag="VFB37_14445"
                     /gene_synonym="purI"
     CDS             complement(14885..18802)
                     /gene="purL"
                     /locus_tag="VFB37_14445"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZO23700.1"
                     /translation="MLQLPGAPALSAFRIAKLVARLAAWEPAVKALDARFVHFIDTAR
                     ALSESQLDILRQLLTYGPHIKPASSPLVGENLLVVPRFGTISAWSSKATDIAHVCGLK
                     DVRRIERGIEYRILDSRPLGPEGLANLAPALFDRMTETVLFDSQHAARLFDHAPARPL
                     ARVALGGSAGRPALERANQELGLALSADEIDYLVHSFRALGRDPTDVELMMFAQANSE
                     HCRHKIFNADWIIDGRRREESLFAMIRYTHKCSPGGVLSAYRDNAAVLEGNPGTRYFP
                     DPHTGIYGGHLEPIDILIKVETHNHPTAISPFPGAATGSGGEIRDEGAAGRGAKPKAG
                     LTGFSVSNLRIPGYERPWESDFGRPERIAAALDIMIEGPIGAAAFNNEFGRPAIAGYF
                     RTLEHCLPGDLPGRARGYHKPIMIAGGLGNIRRAHVDKAEVPLGAKLIVLGGPAMLIG
                     LGGGAASSLGSGASDADLDFASVQRGNAEIQRRAQEVIDHCWALGEGNPISLIHDVGA
                     GGLSNAVPEAVAHSKRGARVDLRAVPSAESSLSPMELWCNEAQERYVLTIEASRVREF
                     AAVAARERCPYAVIGEIDGSGRLVVHDPMFEENAVDMPIEVLLGKTPRMLRDVRSAKP
                     PRQPFEVTGLDLSEATYRVLRLPAVADKTFLITIGDRTVGGLISRDQLVGRWQVAAAD
                     VGVTLSDHYGHAGEAMALGERAPVALLDAPASGRLAVAEAITNILAADIDSLARIRLS
                     ANWMAACGEPGEDAALYETVYAVARELCPALGIAIPVGKDSLSMKTSWAAPAEGAAGT
                     VQKSVVAPVSLIITAFAPVNDVRRTLTPVLITDERPSSLWLIDLGGGRNRLGGSALAQ
                     VHGCLGQEPADLDDPQRLVRFAAAMVELRAADGLLAYHDRSDGGLLVTLLEMAFAGHC
                     GLDIELPTARGGAFEQLFSEEPGVVVQVSAAQEDRLTEILSRHGLRKNALHLGSPTKE
                     LRIRMRVGESVLDESWVDLRRAWSETSWRMRRMRDDPQSADEEFAAQSASDDPGLTVS
                     LSFDPGEDVAAPYIAGRARPAVAILREQGVNSQVETATVFERCGFVAHDVHMTDLLAG
                     RRSLREFKGLVACGGFSYGDVLGAGEGWAKSILFHEAVREEFRAFFARSDSFAFGICN
                     GCQMFSALKSLIPGTAHWPRFVRNRSEQYEARFTLVEILRSPSVLLQAMEGSFLPIAV
                     AHGEGRAEFPSDTAAATCAASGLVGFRYVHNDRRVASSYPFNPNGSPHGIAALCNENG
                     RVTITMPHPERSFRYAQNSWHPHDVGEYSGWMRLFRNARRFVD"
     gene            complement(18817..19152)
                     /locus_tag="VFB37_14450"
     CDS             complement(18817..19152)
                     /locus_tag="VFB37_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HZO23701.1"
                     /translation="MAISHIFRIMFVNQGKVYEIYARRVSHGELFGFVEVEELVFGER
                     ATVVLDPAEERIKGEFEGVKRTYLPLHSILRIDEVKKQGVSKITALEGGTNVSQFPMP
                     MYSPPQDKK"
     gene            19277..20533
                     /gene="tagH"
                     /locus_tag="VFB37_14455"
     CDS             19277..20533
                     /gene="tagH"
                     /locus_tag="VFB37_14455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HZO23702.1"
                     /translation="MTLRLRIVSDHRRLLGDRSTVLFSENGGTIGRSADNDWVLPDPV
                     RYVSAHHARVHFRDGHFFLEDVSTNGVFVNDDDKPLARAGAEGHQLRSGDLIRMGDYQ
                     IVAALETEPEEATTPKPDTGAGQVPTSIHALRTLGRAAQTDIGAMLDLDDLLVPDSSP
                     SDSFRPVNAYGQAVAQAPARAAVARPTPAPAVPTEEEAAIERRIARLARAAARDPKNG
                     PSVPALFDVSSGLQAFCRGAGIEADKLPADAQTRMLHLAGQLFRESLVGLKELERARN
                     DTRNRYRIELPTETDDPRPSLARSTVEDLLIELFLQHESRQMDAVGWLRDAIAEGKTH
                     ENAAARATRAAFVEFLDRLDPAELEARFERAARRGKARSADKAQYWELFTSFYRNLIE
                     MPADHLPHTFVEAFAAAYREHVRKSE"
     gene            complement(20714..20806)
                     /locus_tag="VFB37_14460"
     tRNA            complement(20714..20806)
                     /locus_tag="VFB37_14460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20769..20771),aa:Ser,seq:cga)
     gene            20960..22420
                     /gene="mltF"
                     /locus_tag="VFB37_14465"
     CDS             20960..22420
                     /gene="mltF"
                     /locus_tag="VFB37_14465"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HZO23703.1"
                     /translation="MAAAQSVLLLVAAFLLPSCTEHPSALDSIRARNEIRVVTLNTPT
                     CFYYGAQGAEGLEYKLAKTFAAELGVRLVMYPVSGEHAMQAELAAGRADIAAAQLTAT
                     RDWNRVGDAAAPYAQVAQFVVYRRDEKRPRSTLQIEAAKLAVRAGSPQEQMLERMKKT
                     VAPGLQWVDTAPNNADPLEDVNSGDAAYALVDSREFSFARHLYPDVLIGFSLPEARSV
                     QWITRRGARDLLAAVNRFFRLLAESGRLARLQQEETGNHRSFEYEESREFQAHLGERL
                     PQFQPWFEAAAAQTGVDWHLLAAIGYQESKWDPKAQSDDGALGVMMLTSDTAAAMGIS
                     DRTNPQQSITAGALYLADVRSKIPTRIPEPDRTWLTVAAYNVGFGHLEDARIIAQSRG
                     QNPDSWADVRQQLPLLAQERWYSKAKRGYARGWEPVQFVDRVQRFLTLLSWQPGEGVG
                     APPAVTPTTPPTLPASLHAPVPPATPASSPSSSPAS"
     gene            complement(22435..22914)
                     /gene="tadA"
                     /locus_tag="VFB37_14470"
     CDS             complement(22435..22914)
                     /gene="tadA"
                     /locus_tag="VFB37_14470"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZO23704.1"
                     /translation="MDPSRTAPAIPSDIAFMQRALEVSERARESGEVPVGAVLVLNGQ
                     ILADGANRPIVAHDPTAHAEIEALRAGGRTLGTYRLLDTTLYVTLEPCAMCAAAIVHA
                     RVRRLVFGAWDTRAGAAGSTIDLFALAGQNHRVDVFGGVLAAECGDRLKAFFAQRRL"
     gene            23087..>23272
                     /locus_tag="VFB37_14475"
     CDS             23087..>23272
                     /locus_tag="VFB37_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23705.1"
                     /translation="MHTHSFGEYVRRIDRDDWRGVGELMLQSASKLADGGADFLICPD
                     NTIHQALPDLQARSPLPW"
BASE COUNT         4422 a         7880 c         7219 g         3751 t
ORIGIN      
        1 ccgcgccagc tctttggagc ggcgagcgcc atgaaaacga cgccggcaac gggatccacg
       61 acaggtggtg cagcgtagcc aaacagcaag aggcccttct aaagttccgc agctgcgtgc
      121 cgctaacctc tgcgaagcgg caggcaggcg cccctgcgcc tgcgatcgag cggcaaactc
      181 ttgccgtgcg cgtgatcgcc ctcacgcccc gagagccgac gaccctccca ccgatgcccc
      241 cgcggcccgt ggcatgcgga ttgcaatccg tccgtcaggt cattcttttg acggatcgta
      301 cgcagcatgc cgctcaatct cgacacgtac ctgggcgttc acgcggacgc actgaagttg
      361 caatcgcggc gcatggaagt gctcgcgaac aacctcgcca atgtcgacac gcccaacttc
      421 aaggcgcgtg atatcgattt ccgcagggct ctggcggctg cgggcggtgc ggatgccccg
      481 gtgaaaatgg cggctaccga tccccgccac ctcggcgccg acccggcatc ggtccaggag
      541 gctgacctca agtaccgcgt cccgctcgca ccggccctgg atgggaacac cgtggatgcg
      601 cagcaggagc aggccgcttt cgctgacaac accgtgcgct atcaagccac gctgacgttc
      661 ctgtccacgc gcttcaagaa cctcctcacc gccatcacgg gctcgtgagc gatcgaccgg
      721 agcaactgac atgtcctctt tcaagatatt cgacatcgta ggatcgggac tcagcgccca
      781 gtcggtgcgg ctcaacacgg tcgccagcaa cttggcgaat gccgacagcg tgagcggcga
      841 tcccaacacg gtatacaagg cacgccatcc ggtcttccag gccgtgaagg cggcggtcgg
      901 ccagagcgca gaccccatcg atcgcgcgaa cggtgcctcg gtgcgcgtca gcggaatcgt
      961 ccagagcgat gcacccccca cggagcgcta tgaccccggc aatccgcttg ccgatgagca
     1021 tggcaacgtg tatgcgccga acgtcaatgt catagaagag atgaccgaca tgatctcggc
     1081 ctcacgcgcg taccaggaca acgtggaggt gatgaacacc tccaaggacc tgatgctcgc
     1141 gaccctgaag ctcggcgact gagcgccaca cgaaggtaac ccatgtccac cacgaacccg
     1201 atcaccggca tcaccaccgg catcgctacg tccacgaaca gcgtgccggc caacatgcag
     1261 atcgaccaga gcgatttcct gcagctcatc accacacagc tgcagaacca gaatccgctg
     1321 cagccggccg atccgactca gttcgtatcc cagctcgagg gcatgagcga agtctcgagc
     1381 atgcagagca tgcagagctc gctgcaggcc tcgcaggtga tgagcggcac ggcattgctg
     1441 ggacactcgg tgctcgcgcc tggcgccagc gccaatctga ctgccggagg ctcgatcagc
     1501 ggtgccatca cggcacccgc cggcgcgagc aacctcgcgg tgacgatcag caactcctcg
     1561 ggcacgccgg tgtcctcgtt caacgtcact ccgcaggcga ccgggctcac tcaattcagc
     1621 tggaacggcc atacggccgc cggcgccgcc gccgccggca tgtatcagat tcaggtcaat
     1681 gccaccgtga atggaaccac ccaacccgtg aaccccatgg tcgtgagcaa ggtacagagc
     1741 gtgacgctcg atccgagcac gcaggcggtc gatctgttga ccgacagcgg cacggtagcc
     1801 ctgagctcgg tcgtctccat tctctagcgc acccccgcat tccatttcaa ggagttctca
     1861 atgtccttca atatcgctct gaccgggctc gatgccgcca accaggatct gtcggtcacc
     1921 gccaacaatc tggcgaatgt cgccaccacg ggcttcaagg gctcgcgcgc cgagttcgcc
     1981 gacctgttcg cctccaccca gcagggtgtg agcgccacgg cggtcggcaa tggcgtcgcg
     2041 gtctccgagg tcgcgcagca gtttacccag ggcaacatcg agacgaccgg caacaatctc
     2101 gacctggcca tcagcggcaa tggcttcttc accatgagtc agaacggcgc gctcagctac
     2161 acccgcgacg gtgagttcca ggtggacaac agcggcaacg tcgtcaacgc tcagggcgcc
     2221 aatctgcagg tctaccctcc ccttacgacc ggaggcttca ataccggcgg tctcaccaac
     2281 ctttcgctca ccaccgggga gagcgctcct caggcgacgg ccaatgctca gatcactgcc
     2341 aaccttccgg ccagcgcgag ccagccggcc gatgcggtct tcagcccaac cgacccggca
     2401 agctacaaca actcgacctc gctgaccgtg tacgactctc tgggtgccgc tcacacggcc
     2461 acgctgtact tcatcaaggg cgcaacgacc aacgattggt cctcgcagct ctacgtggac
     2521 gggaccgccg tcggtacccc gcaggcgctc gactattcga acaccggcgc gctgaccaca
     2581 ccggctaacg gccaggtgac gttcccggcc tatacgccgg cgacgggcgc cgcgcctctc
     2641 aacatgacct tcaatttcag caataccacc cagttcggcg acaacttcgg ggtcacggct
     2701 gtatcgcagg atggcttcac caccggcaag ctgactggta tcagcatcga ctccaccggg
     2761 gtcgtgcagg cgcgcttcac gaacggccgc tcggtcaatc tcggccaggt ggcactcgcg
     2821 aatttcgcca acccgcaggg cctgcagcag ctcggcaacg ccagctgggc gcagaccaat
     2881 gcctcgggag ccgccgtggc gggcgtagcc ggcaactcgg gcttcggcac catccagtcc
     2941 ggatcgatcg aggagtccaa cgtggacacc acctcggcgc tggtcgacat gatcgtcgcg
     3001 cagcgcgact tccaggcgaa tgcgcagatg atccagacca acgaccagat cactcagacc
     3061 atcatcaaca tccgccagta gagctgacgc agacaaggcc ggagtgagtc atggacaagc
     3121 tgctgtatgt cgccatgtcg ggagccaagg acaccttgcg cgcccaggca gccaactccc
     3181 acaaccttgc gaacgcgagc accacggggt tcaaggccga tctcagcgca ttccagagcc
     3241 gcgccgtcgc cggaccgggt tatgcatcgc gggtgtatgc gaccgatgcg accgtgggct
     3301 gggaccatgg ggtcggagcg cagaccacga caggcaatcc gctggatgtg gcgatccagg
     3361 gttccggctt cattgccgtg caggatgtga ccggtcagga ggcttacacg cgtgctggcg
     3421 atctgcacgt agatccgggt ggacagctgc tgacggcgac cgggcttccg gtgatgggcg
     3481 atggcggccc gatcagcgtg ccgccttcga cttcgatcag cgtggcggcc gatggcacgg
     3541 tttccatcgt ccctcttgga cagtcgccgc agacggttgc taacgtcgcc cgcatcaagc
     3601 tcgtgaatcc gccaagcagc accatcgagc gcggatcgga cggtctgttc cgcactactg
     3661 acgggcagcc cgcgacggca gatgcttcga ccacgatcgc ctcgggcgtg ctcgagtcga
     3721 gcaatgtcga tctcgcgagc tgcatggtca acatgatcga gctctcccgc cgcttcgacc
     3781 tgcaggtcaa agccctgcac acggccgaag atgacggcgc cgcctccgcg aagctgttgc
     3841 agtcgagctg agccggcgaa tccctttcca ccgacaggta agcacctatg gaaccagcac
     3901 tctgggcagc gaaaaccggt ctcgatgcgc aacagacgca gatgaccgtt accgcgaaca
     3961 atctcgcgaa cgtcgccacg aacggcttca agaagagccg tgccgtgttc gacgatctgc
     4021 tctaccagaa cgtctcgcag gtcggagctg cgacttccca gagcacggtc tctccgaccg
     4081 ggttgcagct gggcacgggc gtacgggtcg tctccaccga gcgcagctat acccagggca
     4141 acctcgacaa caccggcaac tcgctcgacc ttgcgatcca gggacgaggc ttctttcagc
     4201 tcctgcagcc cgatggcacc ctcgcctaca cgcgcgacgg cagtttccag gtgaacgcgc
     4261 agggccagct cgtcaactcg agcggctatg cggttcagcc cgccatcacg attccaacag
     4321 gggcgcagag cgtgaccatc ggcagcgatg gtgtggtcag cgtgctggtc gccggacaat
     4381 cggccccgac ccaggtgggc agcattcaac tcgccgattt catcaatccc gccggcctgc
     4441 agacggtcgg cggcaacctc gccgtcgagt ccgcctccag cggcccgccg cagaccggca
     4501 caccgggact caccggtttg ggcacgctag tccagggatc ggtcgagagc tcgaacgtgg
     4561 acgtcgtcga agagatggtg aacatgatcg agacgcagcg cgcctatgag atgaattcca
     4621 aggcagtgca gacgaccgac cagatgctgc agtacgccac cgagaacatg taagccgatg
     4681 atgcgcttcc tatccatttc cgcacttgcc ctcggtcttc tggccgccct gggctgtcat
     4741 acggtcaagc cgcccaagga cgatgatggc atgagctggg cccaggagcc ggcgaccccg
     4801 cccaccaacg gggccatctt tcagaccggg cgcgaggtcg cgctgttcga gaacccgatt
     4861 gcccatcacg tcggggacat cgtcacgatc gtgctcaacg agcaaaccgc tgcacagaag
     4921 agtgcgacca ctacgaccac caagaacacc agcgacacgc tcccgggcat gacgctgctc
     4981 ggcaaggctg cgaccatcca cggtacgccg atcctgagca acaacatcaa cgatgccacg
     5041 aagttcgacg gcgaaggcga cagcaagcag agcaacagcc tgagcggctt catcactgcg
     5101 accgtgatcc gggtgctgcc gaacggaaac ctgttcatca agggcgagaa atggatcggc
     5161 atcaaccagg gacaggagta tgtgcgcgtg agcggggtca tccgtccgat cgatctggcg
     5221 ccggataatt ccattccctc gcaacgcgta gcgagcgcca agatttccta cgggggcaag
     5281 ggtgcgctcg ccgatgcgaa cgcacagggg tggctctcgc gattcttcaa ctcaccgtgg
     5341 acaccgttct gatcatgccg acactcagtg ctcccagatc cgctttgctg tgcgccctga
     5401 tcgcgctcgc ggtgctcgcc gcaggcacgc caccggcgca ggcggaacgg atcaaggaca
     5461 tcgcctcggt ctccggggtg cgcaccaatc agctcgtcgg ctacggcctg gtggtcggac
     5521 tcgatggaag cggggatcag acgagccagg cgcccttcac gatccagagc atcgccaaca
     5581 tgctggcgaa gttcggcgtg accatgccat ccaattccaa cccgcagctg aagaacgtcg
     5641 cggcggtaac cgtgaccgcg gacctgccgc cgttctccaa gaccgggcag accatcgatg
     5701 tgaccgtcgc ctcgatcggt aatgccgcga gcttgcgcgg cggcgatctg ctcatgacgc
     5761 cgctgcgagg cgtggacggt caggtgtatg ccttcgccca gggcagcgtc gtcgtgagcg
     5821 gctttggtgt gaacggcaag gatggctcga aaatctccct caacgtgccc agctcgggcc
     5881 gcatcccgaa tggggccagc gtggagcggg aggtgcccat caaattcgcc gccgatcccc
     5941 atctgacgct caatctgcac acgccggact tcaccaccgc ggcgcgggtc gcagcgggta
     6001 tcaacaaggc tctcggcagc ggcactgccg atgcggtcga tgcggtctcg atccgggtat
     6061 ccgctccggt ggaagccgat cagcgcatcg cctacctggc gacgctcgag gccatcgaga
     6121 tcgagcctgg cgaggccccg gcgcgcgtga tcatcaactc gcgcaccggt acggtcgtga
     6181 tcagctcaca cgtgaaggtg atggccgcag ccgtcgctca cggctcactg tcggtcacga
     6241 tcacggaacg ctcggacgtc agccagccga attcctttac caatggcacg acggccaccg
     6301 ttactccgcg cagcacgctc gatgtgcagc aggcggatgc gcgcatgttc gtattccagt
     6361 ccggcgtgaa tctcgatgag atcgtgcgcg cggtgaatca ggtgggcgca gctccggggg
     6421 acctggtcgc cattctcgag gccctgaagc aagcaggcgc cctgcgtgcg gagctgatcg
     6481 tcatatgaga cgcggcggcg cgatctcagg agggccgcgc gcgcgggcgc gtccaccctt
     6541 gccatgagtc tggtgaaaga cgctgcgctg ccttccacgg ccgctccggc ctataccgat
     6601 ctggccggcc tcagcgcttt gaaacagggc gccgcgggtc atgatccgag cgcaatccgc
     6661 cgcgtcgctc aggaattcga gagcctgttc acgcgcatga tgctcaagag catgcgcgat
     6721 gccgtgggcc cggacccgat gttcggcagc gatcaggagc agatgtatca gggcatggcc
     6781 gatgaccagc tctcggtcca actctcgcgc ggtcgcggac tcgggctcgc cgacatgctc
     6841 atgcgccagc tgcagaagct cggcgtgccc ggagcggccg cggcagctca gaaatcctcc
     6901 acggcccggg ccggcagcgc tggcgccgcc gcctatggcg ccgtgcagcg cgcagccagc
     6961 gcgagcccgc cggccagcag cgcgcagcaa gccagtttca tccaggggct gtggcccgag
     7021 gcgcagcagg caggacaggc gctcggagtg gacccgcgca acctgatcgc ccaggcggcg
     7081 ctcgagacca actggggccg caacgtgccc cagggctccg gcggggccac gagcaacaat
     7141 ctgttcgggg tcaaggcgag tgattggaag ggccccgcgg tggcggcgac cacgcaggag
     7201 taccagggcg gcgccgcagt gagcacgaac gcacagtttc gggcctacgc ttcgccggcg
     7261 gagagcttcc gcgattatgt cgcgttgctg cgcgacaacc cccgctacgc cgctgctctc
     7321 aacacgggcg ctgacgttca cgcgttcgcg gccggcctgc agcgcggcgg ctatgcaacc
     7381 gacccggatt atgtccgcaa gatgagcgct acggcgctga atgtgacgct cgcgcagaac
     7441 gtgagccagc tcaagtcggc ggacgccgcg ccgataaccg gcaacacggg cacgctctaa
     7501 gggctttgaa ggatgagcga catactttcc accagcgtct cgggtctgct cgccttccag
     7561 cgtgccttgg atgtcacgag caacaatgtg tcaaacgccg cgacgccggg ctacagcgtc
     7621 gagcgcgtcg agcttgccga gaacccggcc caggcgaccg cttcgggcta tatcggcagc
     7681 ggcgtcaacg tccagagcgt gacccgctct tatgatgagc tgctggccgg ccaggtccgc
     7741 tcctcgcaaa gcagctactc gagcttcaac acgtttgcga cgcaggctgc gcaaatcgac
     7801 aatatgttga gtgactcgag caccggcctc acggccagcc tgcagaactt cgtgaatgcc
     7861 ctgcagaccg tggcgaactc gccctcctcg accgcgcagc gccaggtgct gctcagtcag
     7921 gctcagtcgc tcactcagca gatgcagaac tacgactccc agctgtccac ctacagcgcg
     7981 ggtgtcgagt cacagatcac cagcggcgtc agtcagatca ataccctggc gagcggcatt
     8041 gccaacctca atgggcagat cgcctcggcg ctggcgggca cgggccagcc gcccaatgac
     8101 ctgatggatc agcgcgacag cctcatcgac cagttgagcc agtacgtgac ggtcaacacc
     8161 gccacgcaga gcgatggctc catgaacgtc tatatcggcg ccggccaggc gctggtcacc
     8221 ggcagccagg ctcagaccct gaccgccatt caggacccgt acgatgcctc gcaacacgac
     8281 atcgggatcg tgagcggggg caacacggcg gacgtcacct ctgagatcag cggcggctcg
     8341 ctcggcggcc tgctcgcggt gcgtactcag gtgatcgagc ctacccagaa cacactggga
     8401 cagttcagcg tgggactggc gagtatcgtc aatcaggcac agcagtcggg catggatctg
     8461 accggctcga ccggccagcc catgttttct gtcggcggcg tgcagatcct gccggcctcg
     8521 accaaccagg gcaccggagc ggtgagcgcg acgcgtgcca gcctgagcgg cctgacggcc
     8581 gatgactaca agctcacctt cagcggcggg gcgtggcagc tccaggacac cgcctcggga
     8641 cagagtgtgg cgatgaccgg agccggcacg aacgccagcc cgttccaggc cgccggccta
     8701 tcgatcgtgg tgagcggcgc ggccaccaac ggggacagct ttctgatccg ccccacggcg
     8761 gcggcgaccg cgggtctgtc cgtgctgctg agcaaccctg cccagatcgc ggcggcctcc
     8821 tcgatccaga cgacggccgc cacggccaat atcggcaccg gtaccgtctc cggcagcgcc
     8881 atcaccgacc cgagcacctg ggttccgggc acctacacga tctccttcag cagcgccagt
     8941 cagtaccagg tcaccaacag cggcgggaca gtcgtcgctt ccggcaccta taccgccgga
     9001 accccgattg ctttcggcgg cgctgcggtg acgctcacgg gcgcccccgc ggccggcgat
     9061 acgttccagg tgggcgcggc gagcccgtcc aacaccggcg acaacagcaa cgccttcgcg
     9121 atgatcgatg cgctgtcggc ctcgagcttg aacggtggca cgacctcgct gacgagcgtg
     9181 gccaacaatc tcgtcagtca ggtcggtgtc ctcacgcagc agtcccaggc gaacgcctcc
     9241 gcgcagcaat cggtgaatca atccgcggtg gacacgcgca acaatctctc cggagtcaat
     9301 ctggatgagg aggccgccaa gatggtgcag taccagcagg cgtaccaggc ttgcgcccag
     9361 ctcatccaga ccgcaggcac catgttcaac tccctgatca cggccatcag caacggctaa
     9421 tcatgagaat ctccaccgtc accttccagc aggacgcgct cgggcagatg gaaactctgc
     9481 aggccgacct ctccaagaca cagaaccagc ttggaacggg caagaagctc aacagcgccg
     9541 ccgatgaccc cgccggcatg gcgcaggtca accagctcaa cgtcgagctc tcggcctccg
     9601 agcaatatgt gaccaatggc aacgccgcct cctccaacct acagctggaa gagcaggcga
     9661 tgtccgatgc caccaacacg ctgcagagtg cgcgagacct cgccgtgcag gcgaacaact
     9721 cctcactgac ggccgcgcag cgcacggaca ttgctaccca gctcagccag cagctgcagg
     9781 atctgattgc gatcggtaac cgcaccgaca gcaacggcga ctacctgttc tccggtgagg
     9841 cgagcggcac gaccccgttt gcgcaggttg gcaatgcggt gacctattcg ggctcgagcc
     9901 aggttcgcca ggttcagatc agccccaacc agcgcatctc ggcgggggac accggggcgt
     9961 ccgtgttcat gaacgtgccg gccggtaacg gcaccttcac caccgccgcc ggaacgacca
    10021 ataccggtgt tggcgccata ggcgttgggt ccgtgaccaa tgccgccgcc tgggtgcccg
    10081 acacctacac tgtgaagttc acgagtccga gcgcttacac cgtcaccaac agctccggga
    10141 cgacggtcgc ttcgggcacg aatttcaccg acggacagtc gatctccttc aacggtatcc
    10201 aggtcgcgat ctccggcacc ccggccggcg gcgatacgtt cacgatcgcc aaggcgggca
    10261 actccagcgc cttcaacacg ctgcagggcc tgatcaccac cttgaattcc cccaatctga
    10321 acaacgcgca gctcgcgact cagatcggtg gcgcactgca gcagatcgac aacgcgatct
    10381 ccaacctgaa tgacgtctcg gcttcggccg gggcgcgtgt gaactcgatc acctcatcgc
    10441 aggcaaccgc ccagaccacc cagacgaatc tgagcggggc gatctcgagc ctctccgatg
    10501 tggactatgc cgcggccatc acgcagctca acaccgagga gctcagcctg caggcggcgc
    10561 agcagtccta cgcctccatc gcgcatctct ccctgttcga ttacatccac agctgaggcg
    10621 ctcgcgcgcc agcgcccacg cgatgactgt catgcagccg gatccgcagt tctcgtcaca
    10681 ggacgcggcg attatgtcga acggcacctg caggcgcgtt gggcttgatt gcgacgattg
    10741 tgatgcgctc tccagttccg cccagctcgc gactcaagtt caccgggccc gcaccgataa
    10801 ccttcctgaa cgcaccgtgg tcaggagcca cgcgccaaac agagcgacgg acagttgtcc
    10861 ggcgcctgaa gtagtacaca tcaggagtct cagacaatgt cactagtcct aaacacgaac
    10921 tacagctcga tggtcgcgca gaacagcctg accgcttcgg gtggtcagct cgccacggcc
    10981 ctgcagcagt tgtcctctgg tttgcgcatc aacacggcgg cggacgacgc ggccggctat
    11041 gcgatcagcc agggcatgac ctcgcagatc aacggcatca accaggcgtc ccgcaacgcg
    11101 aacgacggcg tatcgctggc gcagaccgct tcgggtgcgc tgcaggagat cactaatgac
    11161 ctgcagacga tgcgcgacct cgccgtgcag tcgctgaatg ccagcaacag cgcgacggac
    11221 cgcaccgacc tcgacgcaca gttccagcag ctcaaggcgg acatcaacag cgtcgcctcg
    11281 cagacgcagt tcaatggtgt caacctgctc gatggcacgt tccagggtgc gaccttccag
    11341 gtcggcgcga atgccggcca gtcgatcacc atggcggcca tttcgagtgc cggctccggc
    11401 aacctgggcc agctgtacgg cgttgccgcg acggatccgg cgacctatgc cggtgggaac
    11461 accgacacgg tgagcttcga tgtcacggtc aacggcacca agtacaccac gagcaccgtg
    11521 acgctgaccg gcacgcagag caccgatctc aaggccgttg cggctgccgt taaccaagcg
    11581 gtgggtcagc agggtgtggt ggcctcggtg gattccaccg gcaagggcat caacctcagc
    11641 agcacgacca gccagacggt gacgatcgcc acgaccgcca cgaagggcac gggaacgggc
    11701 gctgccggcg gtgagacggc tgcctccttc ggcatggtgg aaggctccaa cgccatcacc
    11761 acggccggca acttcctctc caagggtgac gtcacgacgg ttgataattc caacctcgtg
    11821 ctgatccagg tcgatgcggc tctgcagcag ctcgccacca cgggcgcgca gctcggcgcg
    11881 tatcagaacc ggttccaggc tgccatcacg ggcctgaaca cggattcgac caacctgtcg
    11941 agcgcccgca gccagatcca ggatacggac tacgcggcag cgacctccca gctgtccaag
    12001 gcccagatcc tgcagcaggc ctcgaccgcg atggtggccc aggccaacac gattccgcag
    12061 aacatcctga cgcttctgca gaagctgccg taaggctgat ggcccgcgga tcgattcgat
    12121 cgacaaagcc aaggggtggg cgccgcaagg cgcccaccct gcggcctttc tgaacggtcc
    12181 atttttcacg gagccgagtc tcgatggcca gcaccataac cccgacctcg aacagcacca
    12241 gcacgagcgc gccggtctcg attgccagca acagcaccgc cggtgccgcc ggcggctcgg
    12301 tgatcgatgt cagctccctc gtctcgcaac tggttgccgc tacacgcgct ccgcaggacg
    12361 cgttgatctc caaccagacg caagcggtca cgacccagat ttccgccatc ggcacactca
    12421 agggtgcgct gtccgccttc cagtccgccc tcggggcgat tgataccccg tcctccttcg
    12481 cggccatgac cgcgaacacg agcgacagca cgattttctc cgccagtgcg gggtccggcg
    12541 cggttgccgg cacctaccct gtgaccgtct cgcagctcgc ccagggccaa cagctggtct
    12601 ccaaggggtt cgctggcggt tcttcgagca cgatcggcac cggcaccctg cagctgaccc
    12661 tggggagctc cagcttctcc gtcagcatcg acagcaccga tgacacggtg gaggggcttg
    12721 cagcggcgat caatggcgcc ggtgcaaatc cgggcctcac ggcaaccgtg atcaacggga
    12781 ccgacggcgc gcatctcgtt ctgtcttcca ccctcaccgg cgctgccaat acgattggcg
    12841 tcaccgagac cgacggcggt ggcgcgctcg cggccgtgac ctacggttcg ggcaacaccg
    12901 ccaactacac gcagaacgcg gcggcgcaag atgccgcatt cagcatctcc ggcattgact
    12961 atacgagctc gagcaacacg gtgagcaacg ccctggatgg cgtgacactc acgctgctcg
    13021 gcaccactcc gacctcgggt acgggcggcg gcgcggctac gccggaaacc ctgaccgtgg
    13081 ccaacgatac cccgaccatc gaaagcaaca tcagcgcctt cgtgaaggcc tacaacacgc
    13141 tggtgacttc ggtgtccgcg ctgaacagct acgatccgac caccggcacc gccggcccct
    13201 tgctcggcga tgccctcatg tccggtgtgc agaacgagat tcgctccacg ctatacagcg
    13261 tggtgaacac cgggtcagcg gtgtacaact cgctcgccag cgtcggcatc acgaccaaca
    13321 gcgacggcag cctgagcctc aacaactcga ccctgcagag cgctctggcc acggcgccga
    13381 ccgcggtgag tgccctgttc agcggcacca gtggaattgc ctccggtctg aacactcagc
    13441 tcacgggcga actgtcctcg ggtggagtga tcgactcgcg cagccagacg ctggtgcagc
    13501 aggagaatgc cctaagcgag cagaccaatc agctcaacac caagatggac gccctgaccg
    13561 cctcgctgac gcagcagtac tcggcgctca acacgctgct atcgtcactg cagtccacct
    13621 cggcgtacct cagtcaacag ttcgccgccc tgccgctcgt gcagggtcag ccgaacgcct
    13681 agccggccgc tcaagttacc ccgccagcgg ccgctaagta agcagcgaaa atgacggagc
    13741 agaaggccgt gagctcatac gcgcgcaatt ccaagctggc ggcttatcag agcgtctcgg
    13801 tgcacggcgg ggtcgccgag gccgatcctc acaccatggt cctgatgctg atggatgcgg
    13861 ctctggaacg catggccagc gcacggggct gcatcgagcg ccgcgagagc gctcgccaag
    13921 ccggactgct gcacagttgt gtgcagatcg tcggcgagct gcgcggcagc ctcaacatgg
    13981 ccgacggcgg tacgctcgcc cagaacctgg ccgatctgta cgaatacatg atccgcagac
    14041 tgctgctcgc gaacgccgcg agcgacgtcg ctccgctgat cgaagtgcag cgtctgctcg
    14101 atgagatccg cagcgcctgg gtcgcaattg ggccgcaggt gaagcagacg gcgaacgcgc
    14161 ccgctgccgc gacctggaat gcttcggcga acacggcggc tgcgccccaa acgactgcca
    14221 gcggagtcac tcccggcaac tccacggtgt gaggccgctg agggcagctc gggcagtcac
    14281 aaacggagga ccacggcatg gccgaagaga tcgatgcgat agctcgagtc ggtgcggtcg
    14341 gttcgacgga cctggcgccc cctcctgaca gcggggccgg tacaagctcg gcagcgcgga
    14401 ccacggcagc ggccggacag cccgctgaca agttgccttc gcagcccagc gcggcggcga
    14461 ttcaggcggc ggtgcagcag gtgaacgctc acctcgcgac cgtcaatcgc gttctggagc
    14521 tgcacgtgga tgccggaacc gggctcacca tcgctaccat tcgcaatgcg cagacgggag
    14581 cgatgttgca acagtttccg agcgcggaca gcattcacct ggcacagatg ctggcggact
    14641 ggtcgcccgg taagcacgtg ctggtcgatc tgatcgccta gcgctgcggc ctcgggttcc
    14701 cgtgcctacc ggtcgcgggc tcgggctccg tggaactacg tcggcggctc ccctggccta
    14761 ccgccgaggg tttccgctac agccgcggct ctccgctaca gccgcggctc tcccgtgagg
    14821 cgtcgctgtc ccggcgagcc gcggctctcc cgcgcacgcc gctctgaggt cgtcccagcg
    14881 cgcctcagtc tacgaaccgg cgcgcgttgc ggaacaggcg catccagccg ctgtattcgc
    14941 caacatcgtg cggatgccag gagttctggg cgtagcgaaa tgaccgttcc ggatgcggca
    15001 tcgtgatcgt tacacgtcca ttctcgttgc agagtgccgc aattccgtgg ggcgagccgt
    15061 ttggattaaa cggatagctc gaagcgacac ggcggtcgtt gtgaacatat cgaaagccca
    15121 ccaggccact ggccgcacac gtcgccgccg cagtatcgct cgggaattcc gcccgcccct
    15181 caccgtgggc cacggcgatc ggcagaaaag agccctccat agcctgcagg agcaccgagg
    15241 ggctgcgcag gatctccacg agagtgaacc gagcttcata ctgctcgctt cggttgcgca
    15301 cgaatcgcgg ccagtgtgcg gtacctggaa tgaggctctt cagcgcggaa aacatctggc
    15361 agccattgca gatgccgaat gcgaagctgt cggagcgggc gaagaacgca cggaattcct
    15421 cgcgcacggc ctcgtggaac aggatcgact tggcccagcc ctcgcctgct cccagcacat
    15481 cgccatagga gaagcccccg caggccacga gacccttgaa ctcgcgcagc gagcggcggc
    15541 ccgccagcag atcggtcatg tgcacatcat gggcgacgaa gccgcaacgc tcgaacactg
    15601 tggcggtctc gacctgactg ttgaccccct gctcgcgcag aatggcgacg gcggggcgcg
    15661 ctctgccggc gatatacggc gcggcgacat cctcgccggg atcgaagctc agcgaaaccg
    15721 tgagccccgg atcgtcggat gcgctctggg cggcaaactc ttcatcagcg ctctgcggat
    15781 catcacgcat gcgccgcatc cgccacgaag tctccgacca cgcccgccgc agatcgaccc
    15841 agctctcatc gagcactgac tcgccgacgc gcatccgtat gcgcagctct tttgtgggac
    15901 tcccaaggtg tagcgcattt ttgcgcaaac catggcggct cagaatctcg gtgagccgat
    15961 cctcctgcgc agctgacacc tgcaccacga caccgggctc ctcggagaag agctgctcga
    16021 aagcgccgcc ccgtgcggtc ggcagctcga tgtccagccc gcagtggccg gcgaaggcca
    16081 tctcgagcag tgtcacgagc aaacccccat cggagcgatc gtgataggcc agcaggccat
    16141 cggccgcgcg cagctcgacc atggccgctg caaaccgcac caggcgctgc ggatcgtcga
    16201 ggtcggccgg ctcctgaccc aggcagccat ggacctgggc gagcgccgag ccgccgagcc
    16261 gattacgccc gccgccgaga tcgatcagcc aaagggagct gggccgctcg tcggtgatca
    16321 gaaccggggt cagcgtgcgg cgcacatcgt tgacgggcgc aaacgcggta atgatcagcg
    16381 agacgggcgc gactacgctt ttctgcacgg tccctgccgc gccctcagca ggtgctgccc
    16441 agctcgtctt catggacagc gaatccttgc ccaccggaat cgcgataccg agcgctgggc
    16501 agagctctcg ggctacggcg tagacggtct cgtagagcgc cgcatcctcg cctggctcac
    16561 cgcaggcagc catccagttg gccgagaggc gaatgcgggc aagcgagtcg atgtcggcag
    16621 ccaggatgtt ggtgatggct tcggcgactg cgaggcgacc ggatgcggga gcgtcaagga
    16681 gcgccacggg agcgcgctcg cccagagcca tcgcctcacc ggcgtgtccg taatgatcgc
    16741 tcagggtcac cccgacgtcc gctgcagcga cttgccagcg gcccacgagc tggtcgcggc
    16801 tgatcaggcc tcccaccgtg cgatcgccga tggtgatcag gaaagtcttg tcggccacgg
    16861 ccggcaatcg cagcacacga taggtggcct cgctgagatc gagaccggtg acctcgaaag
    16921 gctgccgggg cggctttgcg ctacgcacat cccgcagcat gcggggcgtc ttgccgagca
    16981 acacctcgat cggcatgtcg accgcatttt cctcaaacat cggatcgtga accacgaggc
    17041 gtccgctgcc atcgatttcg ccaatcacag cgtagggaca gcgctcccgc gcggcaaccg
    17101 cggcgaactc acgcacacgc gaggcctcga tcgtaaggac gtagcgctct tgcgcctcgt
    17161 tacaccacaa ttccatcggc gacaggctcg actccgcgct cggtaccgca cgaagatcca
    17221 cacgcgcgcc ccgtttgctg tgagccaccg cctccggcac cgcgttcgac aaccctcccg
    17281 cacccacatc gtgaatcagc gagatcggat ttccctcgcc cagtgcccag caatgatcga
    17341 tgacctcctg ggcgcggcgc tggatctcgg cgttgccacg ctgcaccgat gcgaagtcga
    17401 gatcggcgtc cgatgcgcca ctgcccaggg aggatgcggc gcctcccccg agcccgatca
    17461 acatcgccgg accgcccagc acgatcagct tggcgccgag cggcacttcg gccttgtcca
    17521 catgggcgcg acgaatgtta cccagcccgc cagcgatcat gatcggcttg tggtagccgc
    17581 gcgcccgccc cggcagatcg cccggcaggc agtgctccag ggtccgaaaa tagcccgcaa
    17641 tcgccggacg gccgaactca ttgttgaatg cggcagctcc gatcggcccc tcgatcatga
    17701 tgtcgagcgc cgcagcgatc cgctccggtc gtccgaagtc tgattcccat ggccgctcat
    17761 agccggggat gcgcagattc gagaccgaga acccggtcag ccccgctttg ggcttggcgc
    17821 cacgccccgc ggcgccctcg tcccggatct ccccgccgga gcccgtagca gccccgggga
    17881 agggcgaaat ggccgtcgga tgattgtggg tctcgacctt gatgaggata tcgatcggct
    17941 cgaggtgccc gccgtagatc ccggtgtgcg gatcgggaaa gtatcgggta ccgggatttc
    18001 cttcgagcac cgcagcgttg tcccgatagg cggagaggac gccacccggg ctgcacttgt
    18061 gggtgtagcg gatcatcgcg aacagggatt cctcacgcct gcgcccatcg atgatccaat
    18121 cggcgttgaa gatcttgtgg cggcaatgct cggagttggc ttgcgcaaac atcatcagct
    18181 cgacgtcggt cgggtcgcgg ccgagggcgc ggaagctgtg cacgaggtag tcgatctcat
    18241 ccgccgagag cgcgaggccg agctcctgat tggcgcgctc gagcgccggg cggccagcgg
    18301 accctcccaa cgcgacgcgc gcgagcggcc gggcaggcgc gtgatcaaac aatcgtgcgg
    18361 cgtgctgcga atcgaacagc accgtttcag tcatgcgatc aaacagtgcc ggtgcgagat
    18421 tcgccaggcc ctcgggcccc agaggccgcg aatccaggat gcgatattcg atgccgcgct
    18481 cgatgcgtcg gacatccttg agtccgcaga catgagcgat gtccgttgcc ttgctcgacc
    18541 aggccgagat cgttccgaag cgcggtacga ccaacaggtt ctcaccgacg agcggactcg
    18601 aagcgggctt gatgtgcggc ccgtaggtga gcagctgccg cagaatgtcg agctggctct
    18661 ctgagagcgc gcgagcggtg tcgatgaaat gcacgaaccg cgcatcgagc gccttgacgg
    18721 cgggctccca cgcggcaagc cgggcgacca gctttgcgat ccggaatgcg gatagggcgg
    18781 gtgcgccggg aagctgcagc atggagattg tggcgcctat ttcttgtcct gcggcggcga
    18841 gtacatcggc atcggaaact gcgagacgtt ggtgccgccc tcgagtgcgg tgatcttgct
    18901 gaccccctgt ttcttcactt cgtcgatgcg caggatagag tgcagcggca gataggtgcg
    18961 cttcaccccc tcgaactcgc ccttgatgcg ttcctcggcg ggatcaagca ccacggtggc
    19021 gcgctcgccg aacaccagct cctccacctc cacgaagccg aacagctcgc cgtggctcac
    19081 gcgacgggcg tagatctcgt aaaccttgcc ctgattcacg aacataatcc ggaaaatgtg
    19141 actgattgcc atgggctcgg gtgggcgtac tgctaatttg aaggtggcat tatatgtccg
    19201 tgcgcggcgg ggaagtggcg gcacgtggcg gacaggccaa aacgacctca gatcaagcag
    19261 agccggtccg ggaaccatga cgcttaggct gcgcatcgtc agtgatcatc ggcggttgct
    19321 cggcgaccgc agcaccgtcc ttttcagcga aaatggtggg acgatcggtc gctcggcgga
    19381 caatgactgg gtgctgccgg atccggtgcg gtacgtctcc gcgcatcatg cccgcgtgca
    19441 ctttcgggac gggcatttct tcctcgagga cgtcagcacg aacggcgttt tcgtcaatga
    19501 tgacgacaag cccctcgcgc gagccggtgc cgagggacat cagctgcgca gtggagacct
    19561 catccgcatg ggcgattatc agatcgtggc ggcgctcgag acggagcctg aggaggccac
    19621 gacaccgaag ccggacacgg gcgcgggtca ggtgccgacc agcattcatg ccttgcgtac
    19681 gctcggccgt gcggcccaga ccgatatcgg cgcaatgctc gatctcgacg acctgctggt
    19741 ccccgacagc tctccgagcg acagctttcg tccggtcaac gcgtacggtc aggccgttgc
    19801 acaggcgccc gctcgtgccg ccgttgcgag gcccacaccc gcacccgcgg ttcctaccga
    19861 ggaggaggcg gcgatcgagc gccgcatcgc gcgcctggcc agggcggccg cccgcgaccc
    19921 gaagaacggc cccagcgtgc cggctctttt cgatgtctcc agcggcctgc aggccttctg
    19981 ccgcggtgcc ggcatcgagg ccgacaagct gcccgcggat gcgcagaccc gcatgttgca
    20041 tctcgcaggt cagttgtttc gtgagtcgct ggtcggattg aaggagctcg agcgggcgcg
    20101 caacgatacc cgcaaccgtt accggatcga gctgccgacc gagacggacg acccccgccc
    20161 atcgcttgcc cgctcgacgg tggaggacct gctgatcgaa ctgttcctgc agcacgagag
    20221 ccgtcagatg gatgccgtcg gctggctgcg cgatgccata gcggagggga agacacacga
    20281 gaatgcggcc gcacgcgcca cgcgggctgc attcgtcgaa ttcctcgatc gactggatcc
    20341 ggccgagctc gaagcgcgct tcgagcgtgc cgctcggcgt ggcaaggccc ggtccgcgga
    20401 caaggctcag tactgggaat tgttcacgag cttctatcgc aatctcatcg agatgccggc
    20461 tgatcatctg ccccacacgt tcgtagaggc ttttgcggct gcgtataggg agcatgtcag
    20521 gaagtccgag tgacaggccc tttgaatgtg gcggagaggg tgggattgct gcggccgtcc
    20581 tggccgcagg ccctgcgggc ccgacgctcc gcgtcgtcca aaatcgctcc aggcgatttt
    20641 gtcgaaccca gtgcgcacgt ccacacaccc atgccgccac atccaaaagg cctgaagaca
    20701 ggccctttga atgtggcgga gagggtggga ttcgaaccca cgtgccggtt ttacccgacc
    20761 atccgatttc gagtcggcgc cgttatgacc gcttcggtac ctctccggat gcgctgccgg
    20821 gccagccgcc cctgccaggc gaccccgttt tcggggctgg tattctagcc gaacgttgtc
    20881 gcacgaggtg aggttcgcgt tgatacgccg agcgccatgc tcaggtccgc gtgggacgca
    20941 tttcccttgc cggcgaggct tggcagccgc gcagagcgtg ctgctgctcg tcgcggcctt
    21001 cctgctgccc tcgtgcaccg aacatccatc cgccctggac tccattcgcg cgcgtaacga
    21061 gatcagggtg gtgacgctca acacgccgac ttgcttctac tatggcgcac agggcgccga
    21121 aggtctggaa tacaagctcg cgaaaacctt tgccgcagag ctcggcgtgc gcctggtcat
    21181 gtatccggtt tcgggagagc atgcgatgca ggccgagctt gccgcagggc gtgccgacat
    21241 tgcggcggct caactcacgg ctacccgcga ctggaatcgt gttggagatg ccgctgcacc
    21301 gtacgcccag gttgcgcaat tcgttgtcta tcgacgggac gaaaagcggc cgcgcagcac
    21361 attgcagatc gaggccgcga aactcgccgt gcgcgccgga agcccgcagg agcagatgct
    21421 cgagcggatg aagaagacgg tcgctcccgg cctgcagtgg gtggacaccg cgcccaacaa
    21481 cgccgatccg ctcgaggatg tcaattcggg ggacgccgcc tacgctctcg tcgactcgcg
    21541 tgagttctcc ttcgcccgac atctgtatcc ggatgtcctc atcggatttt cactgccgga
    21601 ggcgcgatcg gtgcagtgga tcacgcgccg cggcgcgagg gatctgctgg cggccgtgaa
    21661 ccgcttcttc aggctgctcg ccgagtccgg gcggttggcc cgcctgcagc aggaggagac
    21721 cggcaaccat cgcagcttcg agtacgagga atcccgggaa ttccaagccc atctgggcga
    21781 gcggctgcca caattccagc cgtggttcga agccgctgct gcacagacgg gcgtggattg
    21841 gcacctgctc gcggccatcg gttatcagga atcgaagtgg gacccgaagg cgcagtccga
    21901 tgatggggcg ctcggggtca tgatgctcac ctcggacacc gccgcggcca tgggaatatc
    21961 tgaccgcacc aaccctcagc agagcatcac ggccggagcg ctctacctgg ccgacgtgag
    22021 atcgaaaatt cccacgcgga tccccgagcc ggaccggacc tggttgaccg tcgcggcata
    22081 caacgttggc ttcggacacc tggaagatgc gcgaatcatc gcgcagtcgc gcggccagaa
    22141 tcctgactcc tgggcggatg tccgacagca gttgccgctg ctggctcagg agcgctggta
    22201 ctccaaggcc aagcgaggct atgcccgagg ttgggagccc gtgcagttcg tcgaccgcgt
    22261 gcagcgattc ctgacgctgc tgtcctggca accgggcgaa ggtgttggcg ccccgccagc
    22321 tgttacgccg acgacgcccc ctacccttcc ggcaagcctg catgcgccag tcccgccagc
    22381 cactccggcc tcgagcccat cctcgagccc ggcatcctga gcggaatccg cagctcagag
    22441 ccggcgctgg gcgaagaatg ccttgagccg atcgccacac tcagcagcga gcacgccgcc
    22501 gaagacatcc acgcggtgat tctggccggc cagagcgaag aggtcgatcg tgctgcccgc
    22561 tgccccggcg cgcgtatccc aggcgccgaa caccagccgt cgcactcggg catgcacgat
    22621 tgcggctgcg cacatggcac acggctcgag cgtgacatag agcgttgtgt ccaaaagcct
    22681 gtaggtcccc aaagtgcgac ctccggctcg cagcgcctcg atctcggcat gtgcggtcgg
    22741 atcgtgggcc acaatcgggc gattggctcc atcggcgagg atctgtccgt tgagaaccag
    22801 aacagccccg actggcacct cgccactttc gcgtgcgcgc tcggacacct ccagcgctcg
    22861 ctgcataaac gcgatgtccg atgggatagc gggcgctgtc ctgctgggat ccatgacgct
    22921 aacctagcac gcggccgccg agatccgcgt gacggagtaa aattcccgaa tgaccggaca
    22981 cattggcatc gttgcatgct ccgccgaagg cgcagcgctg tgctatcgaa caatttgcat
    23041 tgaaggtgcg caagtcctcg gcggacacga tcatccggag gtgtcgatgc acacgcactc
    23101 gttcggcgaa tacgtgcggc gcatcgatcg tgacgactgg cgtggggtcg gcgaattgat
    23161 gctgcagtcg gcaagcaaac tggccgatgg cggagccgat tttctcatct gtccagacaa
    23221 cacgattcac caggccctgc ccgacctgca ggcgcgttcc ccacttccgt gg
//
LOCUS       DASRHN010000360        31474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_623561, whole genome shotgun sequence.
ACCESSION   DASRHN010000360 DASRHN010000000
VERSION     DASRHN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 31474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31474
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623561"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="VFB37_14480"
     CDS             complement(<1..2252)
                     /locus_tag="VFB37_14480"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZO23706.1"
                     /translation="MQSQERASRPSKTNGKGATEPASLDLRKLLRALQAVRDGDFSVR
                     LAGDQTGIAGKVADTFNEIVSSNQRLARELERAGQIVGKDGKTRHRMSTDTRSGAWGA
                     MEISVNTLIDDLVWPTAEVTRTITAVAKGDLQQAMSLEVDGRALKGEFLRSATIVNSM
                     IEQMSAFTSEVTRVAREVGTEGKLGGQAAVPGVAGTWKDLTDSVNSMASNLTGQVRNI
                     SEVTIAVANGDLSRKITVDVRGEILQLKEAINTMVDQLRSFASEVTRVAREVGTEGKL
                     GGQAIVPGVAGTWKDLTDSVNAMATNLTGQVRNIAEVTTAVARGDLSRKITVDVKGEI
                     LELKNTINTMVDQLNSFAAEVTRVAREVGTEGKLGGQAVVPGVAGTWKDLTDNVNSMA
                     GNLTGQVRNIAEVSTAIASGDLSRKITVDVRGEILQLKETINTMVDQLRAFASEVTRV
                     AREVGTEGRLGGQAVVPGVAGTWKDLTDSVNSMAGNLTGQVRNIAEVATAIANGDLSR
                     KITADVKGEILQLKESINTMVDQLNAFAAEVTRVAREVGTEGKLGGQAEVKGVSGVWR
                     DLTENVNSMASNLTGQVRNIAEVATAIARGDLSRKITVDVRGEILQLKETVNTMVDQL
                     NAFAAEVTRVAREVGTEGKLGGQAQVEGVGGTWKDLTDSVNTMAGNLTSQVRNIAEVT
                     TAVANGDLSKQITVDVRGEILQLKNTINTMVDQLRSFAAEVTRVAREVGTEGKLGGQA
                     EVKGVAGTWK"
     gene            2717..3022
                     /locus_tag="VFB37_14485"
     CDS             2717..3022
                     /locus_tag="VFB37_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23707.1"
                     /translation="MSASTLGEEVASTPEPRPARLNVAALRRAVMIRMWGGKGCGSPC
                     DFCRVVVTSTDVEYEIDARLDGQIVMLHFHRRCYDAWKSGQEPAEPAPAESSPPSAA"
     gene            3057..3515
                     /locus_tag="VFB37_14490"
     CDS             3057..3515
                     /locus_tag="VFB37_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23708.1"
                     /translation="MLVIASCALAASPPPPAAPAEPQEFQVAHSALLAIEAAPTDDSV
                     ALYIKHVADQTAVNSSDVVVTVDGKAQTISRAKDGSYLVSAAEMRDKGPHTLEITVSH
                     DGIREILSGQLSLPSATEGSFISAHRQIGWWILNIGVVLIAAIALSRRRK"
     gene            3638..3973
                     /locus_tag="VFB37_14495"
     CDS             3638..3973
                     /locus_tag="VFB37_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23709.1"
                     /translation="MAAYRCMRLRRLHISIGISLLLLLAQHGAIVHELSHICRVGNVE
                     VRLQPDTALEKTCELCLAYSQLANPAGHTVHALVFEPAAHAVGTDRPSAAIPAEVPTA
                     RSRGPPLLS"
     gene            4048..6210
                     /locus_tag="VFB37_14500"
     CDS             4048..6210
                     /locus_tag="VFB37_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO23710.1"
                     /translation="MTRALFHGGAASGRTAALCALLLSAPTLAQVEQQASAGIPTVVV
                     TAQHLNEERSRIDTDTGASTYTFDSKVIQSQPGGENQQLNQVMLQVPDAAQDSFGQLH
                     IRGDHNGLQFRLNGIILPDGISFFGQTLPPRLIASMKLLTGSLPAEYGLRSAGIIDLT
                     TKSGALQPGGEVSIYGGSHDSLEPSLFYGGSSGLTTYFVTGDFLRNDLGIESPDGSSD
                     PLHDHTKQYHGFGYFEQILSSSDRISAVIGTSHDKFQIPNLHGVHAMDIGPGLNVNGE
                     TDFLSNDLDENQTEIDAFGILSLQHSRGNLSVQTSFIARYSSLRFFPDPNLGDLLFDG
                     ISQSAYKRDLADGLQSDAAYLLGDAHTLRIGLYVQRDRLTSNTSSQVIRVDASGAQTS
                     IIPLGITDDIGATQWIESGYMQDEWRLAQSLTVNFGLRYDHYRAFSSGSQLSPRINFV
                     WQALQNTTVHAGYSRFFSPPPFELVGGETLDRFLNTSAAPAVSRDDPVTAERSNYYDF
                     GVQQKISPILTLGVDSYYKQATDLIDEGQFGAPIILTPFNYAHGQVYGVEFTGQVTTG
                     QLEAYGNFAIQRAIGKDIVSSQFNFSADDLLYIGDHYIHLDHEQQVTASGGAAYSWAG
                     TRLSGDLLLGSGLRSDLLLPEGSSIPNGDHLPYYTQINLGVSHDFTRAGVRGLSLRFD
                     VINALDRIYQIRNGTGVGVGAPQFGPRRGFFFGVSQAF"
     gene            complement(6251..7945)
                     /locus_tag="VFB37_14505"
     CDS             complement(6251..7945)
                     /locus_tag="VFB37_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HZO23711.1"
                     /translation="MEHPLRLLIVDDRQPDAELSASQITRGGYSCTWRRVETEEEFRT
                     ELREFAPDLILSDFTLPQYNGLAALELAAAEAPDVPFIFVSGTIGEQRAAEALSRGAS
                     DYVPKSELPRLIDSVTRVLGNGDRRSRRRNDAPTELNPSLLDPLTGLPRRAFFTEHLS
                     HQLQRAQDQGLTTVIVVDIERLQAFNETSGPAAGDKLIQCVAERLQRRFGQGADLAHF
                     GGGTFAAVFAERRNHLGPTRDSATAIFGQQFLLADGPVQATLKCGLARYPVDGEDAET
                     LLQHAEAALKTIKDRQEPTSRVAPASAVSDTRRGILEKRLRVALEEQSFFLNYQPIIE
                     RVSGKVTAVEALLRWRDAQLGLVAPHVFLPVLARAGLMCEVGAWVLEQVASDFERWHS
                     TGLPRLRVAVNISAPELSRKDFAAFFLDKTRRAHTLPCIDIEVPEGALRMDLQVLRQT
                     LRALRAEGVRIAIDNFGMSQSSLCRLSELPVDSLKIDRAYISRLTSEPHSHEILIAIL
                     ALARSYGLRTVAEGVENIEQLKTLDALGCEQSQGYLHSPAVSSEELALWVATRSID"
     gene            8249..9322
                     /locus_tag="VFB37_14510"
     CDS             8249..9322
                     /locus_tag="VFB37_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23712.1"
                     /translation="MSSSLTGSSRSFVVPCAGLLALLGTLLVSGCVVTTAPPPRVYAP
                     PPPPPPAAEYSAPQSDEEAVQTAEAPPPLPDYEQPPCPEEGYLWTPGYWRWSPAGYFW
                     VPGTWVAPPRVGVLWTPGYWGFASGVYVFHPGYWGPHVGFYGGVNYGYGYGGVGFVGG
                     RWSGARFTYNTSITHVNTTVIHNTYNETVVNNVTINKVSYNGGAGGIRAMPTAQERMA
                     AQETHVPATASQQQHVQEAARNPGLSAKVNGGHPSIAATPRPGAFNAPGVARAHGAPA
                     GQMARPAPGKPAGANPPGKAPANNPALARRFNNTAQPASSHGGQPAHATQAARAQPQK
                     NAREKKSPQERKQESGGAAGNSR"
     gene            9452..10630
                     /locus_tag="VFB37_14515"
     CDS             9452..10630
                     /locus_tag="VFB37_14515"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HZO23713.1"
                     /translation="MPTRLRHPLVRSRLSSLANRFDRMEWAGAFGDLGTFLPFVIAYI
                     SVVRVPPAGILLSFGLALVLCGLYYRTPFPVQPMKGIGAIAATQAAKSSLITPATLYA
                     ADLVTGLIWLVLGLTGSARYVARWVPGIVVSGIVLGLGMSFMLEGIRMMSADWLIASI
                     GLAGTLLLLASRAIPVMFLLLLFGAVCTLIQNPSAMSALSSVRFALHAPAFALASISW
                     RDLLAATALLALPQLPLTLGNALIATREENNRLFPERPVSESSIATSTGLMNLTSSVL
                     GGVPMCHGVGGLAGQVTFGARTGGAPIIFGTLLLVLALGFSDAVQSLFNIFPHAVLGV
                     ILFIAGGQLALGCGELSRSKVERFVTFITAAFTVWNVGVAFLVGIALTYLAGRGRLRL
                     "
     gene            complement(10639..11244)
                     /locus_tag="VFB37_14520"
     CDS             complement(10639..11244)
                     /locus_tag="VFB37_14520"
                     /inference="COORDINATES: protein motif:HMM:NF019081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HZO23714.1"
                     /translation="MRAWLIVVMMVGMGAAAITPAVAQTPLAQSTGSAVDAVVGNQVL
                     QMRYLTGSPFAGLAGNMDFGALLTEDREFIGSAAMMFDTDIVPLSRLRVQVGPELNLA
                     WLNAAQKTDVFAASVGVNARYELIRRLGLSAFGSAFYSPGVLTFGSAHNMYDFTAGAE
                     MKVSNRLFLLGGYRWLKFTLVNEPDEKVANELFAGVRWQLD"
     gene            complement(11405..12193)
                     /locus_tag="VFB37_14525"
     CDS             complement(11405..12193)
                     /locus_tag="VFB37_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO23715.1"
                     /translation="MTSAIRPLAVVTGASSGIGLELARECASNGFDLFLAADRPLTEA
                     VAAMRELGTQADSLQADLATREGVQALLDRLADRPVDALLANAGHGLGGAFLDQPLEE
                     AQHVVDTNITGTLALIHAVGNRMRARGAGRILITGSIAGFMPGTFQAVYNGTKAFVDS
                     FSWALRNELKDSGVTVTCLMPGATETEFFQRAGMMDTKIGSSKKDDAAGVARIGFKAM
                     MDGEGDVVAGFKNKVQSAVATVTPSSVLAEQHRKEAEPGTARRQ"
     gene            12471..13592
                     /locus_tag="VFB37_14530"
     CDS             12471..13592
                     /locus_tag="VFB37_14530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZO23716.1"
                     /translation="MKRIEAVGEPIEGLSAAAYRVPTDRPEADGTFAWNSTTLILVTA
                     RAGGQSGLGYTYADACIAPLITTVLAQAIAQKNSFDIAGCWWAMQRAVRNLGREGLAA
                     CAISAVDAALWDLKARSLGLALASLLGRCREAVPVYGSGGFTTYSDDELREQLSGWVE
                     QNGCRAVKMKIGSEPARDPHRVTVAREAIGERELFVDANGALSAKEALRFVRECPEAS
                     LRWFEEPVSSDDVAGLHLIRERLPAGIDVAAGEYIYTLDGAEHLLAGDAVDVLQADVT
                     RCGGITGFLAIGWLCESRHIDLSGHCAPSLHRHVACAVPRLRHLEWFHDHVRIEQILF
                     DGAPVAADGVIRPDLSRPGHGLEFKEQDARRFAIQASGS"
     gene            13610..14542
                     /locus_tag="VFB37_14535"
     CDS             13610..14542
                     /locus_tag="VFB37_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23717.1"
                     /translation="MRSRSPASEVPVRSARQLNLGSAILALSVLADSGIEHYRGSFHN
                     RAMYTPLGVASLTLAVSLLGALDTRPRGHRARDAIYALSAATGLAGFGFHLYNVAKRP
                     GGFSWLNFFYAAPIGAPFALVLAGLLGKGAESARSTRAPSPGARPSQAAHFCTHQAGR
                     VLAALSAVGLAGTVAEAALLHFRGAFQNPAMVLPVSVPPVAAALLAAAAVRSTPSAMR
                     PGLVCPRGVRPPGTQPGRRSRGLARWWLKLTALLGFVGVGFHARGIARNMGGWGNWSQ
                     NILNGPPLPAPPSFTGLALAGLAALGLMEDNVDE"
     gene            14535..15182
                     /locus_tag="VFB37_14540"
     CDS             14535..15182
                     /locus_tag="VFB37_14540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZO23718.1"
                     /translation="MSDRYPGYDVLAKRDSPSWNAQTRTVVDARLAVQPDAHTFFSQA
                     EWPSVCAICDRIIPQPPQASRRVPIAALLDAHLCNIASDGYRDARMPPMREAWRLGLR
                     ALDAESQRAHGHRFHELEVTQQSALLRVVQQGHTTDPAWQSLPPDLFFSKRVLHDITQ
                     LYYAHPYAWNQIGFGGPAAPRGYVRMDFDRRDPWEASEARPGREEEARRENARIG"
     gene            15205..16818
                     /locus_tag="VFB37_14545"
     CDS             15205..16818
                     /locus_tag="VFB37_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZO23719.1"
                     /translation="MNGRAPDVFRVGRWVPMRQYSEAEPVDFAIVGTGAGGGTLACKL
                     AEYGFSVVALDAGPYWRPLEDFASDELHQRKLYWTDERIVDGENPLQLGSNNCGKSVG
                     GSTVHFAMVSLRFRPEWFRSRTLLGYGADWPLDWREMWRYYTEVEQALKISGPVRYPW
                     GPPRPRYPYRPHELNAAALVLAKGAEALGIDWTATPLATLSAPRGLAHPCVYRGMCVI
                     GCATNAKQSVLVTWLPRALAAGAEIRDLAMIGRVDHDPNGRVTGVHYFRQGSWHFQRA
                     RNVVVAGYAIETPRLLLNSASSRYPDGLANSSGLVGKNLMVQSNQAVWGVMKEEIRSY
                     KGPPSLAITEHWNYADEGKDFFGGYSYMSQGPLPVVWSGTEAGKRGLWGQQLLDEMEK
                     YNHQVGLKIVGECLPQERNRVTLTDLKDQYGLPVPRVTYSYCDNDKRLIRHSLKFMRK
                     SLEAVDATELWDETDDTCHLNGTARMGADAASSVVDADCRSWDIPNLWICDGSVFPTV
                     GGVNPSLTIQAIACRTADRIRALAARGEL"
     gene            complement(16837..17853)
                     /locus_tag="VFB37_14550"
     CDS             complement(16837..17853)
                     /locus_tag="VFB37_14550"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO23720.1"
                     /translation="MSTDRTETARTVIITGAAAGIGRAIAERFARAGDRVGLIARDAP
                     ALEALSRELQSRGAEVAFEPVDVADASALFAAAESLEQRLGKVDVWVNDAMVTVFSTV
                     ADITPEEFRRVTEVTYLGFVHGTMAALKSMRPRRSGRIIQIGSALAYRGIPLQSAYCG
                     AKHAIRGFTDSLRSELLHEGSPIRISIVELPAVNTPQFDWARAHISHSPRPMGRPIEP
                     EVVADAVFRVARGRWREYWLGGPTLLTILGDAVLPGFLDRYLARTAFGGQQTREPIGR
                     SRRDNLERPVTELHRTRGSFEAEAAHSAPLMVGEIARLGAFAATAIAFLALGVGIGRW
                     RSRR"
     gene            complement(17876..18247)
                     /locus_tag="VFB37_14555"
     CDS             complement(17876..18247)
                     /locus_tag="VFB37_14555"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HZO23721.1"
                     /translation="MIGHRTWAIAEGYIPGRSVSESRELQSHETACILNAADTDANIE
                     ITLYFEDRGPAGPYRVKVPSERTLHLRFNDLQEPERVPRDTAYSSIIRSDVPVVVQHT
                     RLDTRDTHIALLSTVAYPVQG"
     gene            complement(18244..19092)
                     /locus_tag="VFB37_14560"
     CDS             complement(18244..19092)
                     /locus_tag="VFB37_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZO23722.1"
                     /translation="MVTLRQRLAHLWSLTTCAARYWNSDNSSNIGAALAFFCAFSLAP
                     LLVILLTIAGFFLGAKSAYGEVGMQLGALFGPSTAKILLGAVHSAEQTRGLIATIISV
                     VTLMIGATTVLAALQQALEHIWQSDLLVRSGLYNWFRTRLLSFGFILTLGFLLLISLT
                     ISTGLSNLRDRVAAAHPAMVGAIAVLDILVSLTVVSGLFALIYRYMPARRLAWRHVAL
                     GGLLTAVLFDIGRWAIGLYLAHSTQPSAYGAASSFAALLLWLYYTSLIFLFGAEFTAC
                     LGGARK"
     gene            complement(19255..20640)
                     /locus_tag="VFB37_14565"
     CDS             complement(19255..20640)
                     /locus_tag="VFB37_14565"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZO23723.1"
                     /translation="MRSPKAPSINPAIIRSYDIRGVVGQQLGLADAHALGLAYAAHAI
                     ARGKHRIAVSRDGRLSSPDLEAALAAGLTAGGMNVLRLGVGPTPRMYFAVQAAELEGG
                     VMVTGSHNPPEQNGFKLVLDGEPVFGAALKRLVATPATKRPGGATHDLYLGGIEITTH
                     YVRRLAAVAAGAGELHVVWDCGHGAVGTVIGALVARLPGQHTLLNARVDGSFPAHHPD
                     PAVPANLRQLQTMVVAKGADLGIAFDGDGDRIGVVDATGRIIWPDQLVLFLALEILAA
                     HPGATVVADVKSSRVLFDEVERLGGRAVMVPSGYVIVREAMLREKAPLAGEMSGHIVY
                     ADCWHASDDALYVAMRLLAALGNQGCTLAEFRESLPRMSATPELRVACPESRKQATIR
                     EVAARLVARGAKVDTTDGLRVTADEGWWLLRASGTEPKLTARCEAADPDGLERLRGEL
                     LSHLRQSGIRC"
     gene            20754..21626
                     /locus_tag="VFB37_14570"
     CDS             20754..21626
                     /locus_tag="VFB37_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO23724.1"
                     /translation="MSPQVESPKPEFKSQRQPIPGKTEDMEPRPDHGESSYRGSGRLE
                     GKKALITGADSGIGRAVAIAFGREGADVVVAYLDSSEDADARETQRLVRDAGRTCELF
                     RGDLARADTCRELVRCAVASLGRIDVLVNNAAHQKSFKSLEEIPDEEWDKTFAINISA
                     MFHIVKAALPHMRSGGSIINTSSINLDKPSPQLLPYATTKGAIQTFTAGLAQLLAERG
                     IRANAVAPGPVWTPLIPSTMPQEKVKHFGEQVPMKRPAQPRELAPVYVMLASDEASYV
                     SGATIAVTGGKPII"
     gene            complement(21726..22991)
                     /locus_tag="VFB37_14575"
     CDS             complement(21726..22991)
                     /locus_tag="VFB37_14575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HZO23725.1"
                     /translation="MKANCWNGAMDLRVERVPDPKILNPRDAIIQITSTAICGSDLHI
                     YDGYIPTMMRGDIIGHEFMGEVVEVGADVKNLQVGDRVVNPFPIACGNCFFCNSEEFS
                     LCENSNPNAWMAEKLWGRSPAGLFGYSHMLGGYAGGQAEYARIPYADVGPIKVPEHLS
                     DEQVLFLSDIFPTGYMAAENCAIKPGDTIAVWGCGPVGLFAIASAFILGAERVIAIDR
                     FEERLRKARQDCKAETLNYERVSVPEALREMTGGRGPDACIDAVGMEAHAAGFFGAYD
                     RVKKALRLATDRPSALREAILACRNGGTVSLAGVYGGFLDKVPVGAIVNRALTLKSGQ
                     THVQRYLRPLLQLIEEGRIDPSFVVSHRLALDEAPRAYKMFRDKQDECIKVVLKPGKT
                     GGGRDHGGHNGGRDNGEWTETSPANRVLS"
     gene            complement(22988..24055)
                     /locus_tag="VFB37_14580"
     CDS             complement(22988..24055)
                     /locus_tag="VFB37_14580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO23726.1"
                     /translation="MESDPTSARERARGGGGYTRAPLSWAQGKGRAVGAVLQRQSTAT
                     GLGWLSIGLGLSALLAPRTLGEWTGLENRTGLLRAIGARELLSGAGLLARPEDPTWLW
                     SRVVGDLIDIAVLASAGSATPQSRRRRRATLAVVSAIGAADLATALQRTSFTTSPFKA
                     ARGASPDVYLERTILVNKSQEECYAFWREFRNMTRFSPRLESVTHLDSKRSHWSLRLT
                     GNRRMEWDAEITSDVPNERIQWRTLSGAPFQHAGAVHFDKAPGNRGTFVKVSMHYRAP
                     GGALGSILAQVLGPDPFGEIRENLRRFKQLLETGEIPTTAGQPAGRRSVLSRWLPGAG
                     RLREPPPRKTQERSMQQEVRA"
     gene            24388..25464
                     /locus_tag="VFB37_14585"
     CDS             24388..25464
                     /locus_tag="VFB37_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO23727.1"
                     /translation="MRIAQISPLYEACPPALYGGTERVVTYLCDELVALGHQVTLFSV
                     STSRTRAELVSVRDQPLRLDPAVLKSDVAAHLHLLDEVHARAAEFDILHFHTDLLHFT
                     MFEHLAHKTITTIHGRLDMKDLEQVYRRWSRFGLVSISNAQRQHLKGAHWIANVPHGI
                     PRTLCWPNFDVPQGYLAYLGRISAEKRPHLAIELALRARVPLRMAAKVDKADAAYFHE
                     SVEPLLGRPGVEFVGEIGDAHKSAFLGGARALLFPIDWPEPFGLVMIEAMACGTPVIA
                     WNRGSVAEVIEDGITGFIVETEEEALAAIARADKLDRRTIRARFEQRFSSHIMALAYS
                     DVYTRSLSAVPRLPALRPVGTIAS"
     gene            25461..27596
                     /locus_tag="VFB37_14590"
     CDS             25461..27596
                     /locus_tag="VFB37_14590"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZO23728.1"
                     /translation="MKSADETVEPQGLLPRFTLRDADTFLLADALGDIQGGRHDGLFT
                     SDTRFLSRFELQVGERRPSLLGAALSQDSTLFTAHLTNLPLPALGEGSVPKGVIHVER
                     SRTLSRSRVFERLRLKNFSERAAQVPIGFTLSADFADIFEVQGATRKERGVLLPTRRT
                     ANQLALGYRGLDDRERWTYISFSPKPAEWSPEGAKFVFALQPAAAVELFVEIGSEEQA
                     APSAERFKQACEASEKRMQERAGIAAHISTSFRLFSQWTQKSRFDLALLTTDLATGPY
                     PYAGVPWFATQFGRDAIITALQNLWAEPRLAEGVLRFLASTQAHDTSAYRDSEPGKIL
                     HEMRRGEMAALKEVPFAQYYGAVDTTPLFVMLAGAYERQTGDRRIVDEIWSNLKKATA
                     WIERKLAEHDSGFLYYARGERSGLVNQGWKDSQDSIFHEDGSFPTGAVAVVEVQGYAY
                     SALAAMATLARARGEDKQAAHWSERAETLRAAIEQTYWMPEHGFYAIALDAGTDSVCR
                     VAASNAGHLLYCGVPEPERGRSVCAQLLSPRFSTGWGIRTLADDQPRFNPMSYHNGSV
                     WPHDTAIAAAGIARYGGGAEVAALIRDLFEAANHFGMRLPELYCGFRRADAEGPTPYP
                     VACLPQAWAAGSVFMLLQACLGIDIDGGRRTVNITRPQLPVGIESFCIAGLPVAQSRI
                     DLEFQRMGHEVAVVPSGHLDSSIRVLVYL"
     gene            27688..27897
                     /locus_tag="VFB37_14595"
     CDS             27688..27897
                     /locus_tag="VFB37_14595"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZO23729.1"
                     /translation="MARKYGKSSGRKVKRAMHEMKRGKLRSGRSGAKVKSRKQAIAIG
                     LSEARRSGAKVPKKKTGRKSARKKR"
     gene            27932..28864
                     /locus_tag="VFB37_14600"
     CDS             27932..28864
                     /locus_tag="VFB37_14600"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HZO23730.1"
                     /translation="MSLPELTKTALGELRMQMLGVQVLLGFQFQSLFQQRFVELTRGG
                     RIVDAAGLLLLLIALVVLISIPCRHRIVEKGEATPQLLKVTRQAANLALAPLAGGLAC
                     DVWVSIRIDFGPTQSTLMAVVAGLVATLAWFALPRLLRSTPTAQDPPMHPQATPLHAK
                     IEQMLTEARVVLPGSQALLGFQMVVMLTPAFRELPPAARDVHAAALLCNALAIVLLIC
                     PAAVHRIAFRGEDNPRFHGIGSQLIGAALAPLAVSLACETYVGLLKLFGDARNAALAG
                     CLVLLCMLAVWYLLPLGIRKRVQNLPFAPDRAQR"
     gene            complement(29042..29117)
                     /locus_tag="VFB37_14605"
     tRNA            complement(29042..29117)
                     /locus_tag="VFB37_14605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29082..29084),aa:Ala,seq:ggc)
     gene            complement(29324..31180)
                     /locus_tag="VFB37_14610"
     CDS             complement(29324..31180)
                     /locus_tag="VFB37_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HZO23731.1"
                     /translation="MKRTLALRTTYVSLAAVCAAATASAATDLEQKMSNSANWAAQGG
                     DYANERWSDLNQINASNVGKLQVAWTLSTGVLRGHEGSPLVVGDTMYVQTPFPNNVYA
                     VNLKDQTYKWKYEPRQDVNVVPVMCCDTVNRGLAYGDGKIFLQQADTTLVALDANTGK
                     VVWSVKNGDPKIGETNTNAPHVFKDKVITGISGGEFGVRGRLEAYDLKTGKRAWTAWS
                     TGPDQDILVDPKKTMTWTNGRMAPVGKDSSLKSWQGDEWKLGGGTTWGWYSYDPKLNL
                     LYYGTGNPGTWNPSQRPGDNKWSMSIFARDLDTGMAKWVYQMTPHDEWDFDGVNEVPL
                     VDVTIKGKKVPALVHFDRNGFAYTLNRETGALLVAQKYDPDVNWATMVDMKSGRPVVD
                     KKYSPGTSGPDVNVKDICPAALGSKDEQPSSYDRKTGLFMVPTNHVCMNYEPFRIEYT
                     AGQPYVGATLTMFPTPHSHGGMGNFIAWDPAAGKIVWSKPERFSVWSGALTTAGDVVF
                     YGTLEGYLKAVSPKDGHELWKFKMPSGIIGNVFTYEYNGKQYVGVYSGIGGWAGIGMA
                     AGLKEATAGLGAVGGYKDLARFTNLGGSLFVFALPDNVASGATAMRTSTTPR"
BASE COUNT         5445 a        10367 c         9890 g         5772 t
ORIGIN      
        1 tccttccacg tgccggccac gcctttgact tctgcctggc cacccagctt gccttcggta
       61 cccacttcgc gcgcaacgcg cgtcacttct gcggcgaacg agcgcagttg gtccaccatc
      121 gtgttaatcg tgttcttcag ctgcaggatc tcgccgcgca cgtccacggt gatctgcttc
      181 gagaggtcgc cgttcgccac ggccgtggtg acttcggcga tattgcgcac ctggctggtg
      241 aggttgcctg ccatggtgtt tacggagtcg gtgaggtcct tccaggtgcc gccgacgccc
      301 tctacctgcg cctggccgcc cagcttgcct tcggtaccga cttcgcgtgc gacgcgcgtc
      361 acctcggccg caaaggcgtt gagctgatcg accatcgtgt tgacggtctc cttcagctgc
      421 aggatctcgc cgcgcacgtc caccgtgatc ttgcgagaca agtcgccgcg cgcgatggcg
      481 gtggccacct cggcgatgtt gcgcacctgg ccggtcagat tgctcgccat cgagttgacg
      541 ttttccgtca ggtctcgcca gactccgctg acccccttca cctcggcctg gccgcccagc
      601 ttgccttcgg taccgacttc gcgtgcgacg cgcgtcacct cggctgcgaa cgcattgagc
      661 tgatcgacca tcgtgttgat cgattccttc agctgcagga tctctccctt cacatccgcc
      721 gtgatcttgc gcgacaggtc gccgttggcg atggcggtcg cgacctcggc aatgttgcgg
      781 acctggccgg tcaggttgcc ggccatcgag ttcacgctgt cggtcagatc cttccaggtg
      841 ccggcgacgc caggcaccac ggcctgtccg ccgagtcggc cctcggtgcc cacttcgcgc
      901 gccacgcggg tcacttccga ggcgaatgcg cgcagctgat cgaccatcgt gttgatggtt
      961 tccttcagct gcaggatctc gccgcgcacg tccaccgtga tcttgcgcga caggtcgccg
     1021 ctggcgatgg cggtcgagac ctcggcgata ttgcggacct ggccggtcag gttcccggcc
     1081 atcgagttca cgttgtcggt caggtccttc caggtgccgg cgacgccggg caccaccgcc
     1141 tggccaccga gcttgccctc ggtgcccact tcgcgcgcca cgcgggtcac ctcggccgcg
     1201 aagctgttga gctggtcgac cattgtgttg atggtgttct tcagctccag gatttcgccc
     1261 ttcacatcca ccgtgatctt gcgcgacagg tcgccgcgcg ccaccgcagt ggtcacctcg
     1321 gcaatgttgc ggacctgccc ggtcaggttc gtggccatgg cattcaccga gtccgtcaga
     1381 tccttccacg taccggccac gcccggcacg atggcctgac ccccaagctt gccttccgtg
     1441 ccgacctcgc gcgccacgcg ggtgacctcc gaggcgaagg agcgcagctg atcgaccatc
     1501 gtgttgatgg cctccttcag ctgcagaatc tctccgcgga catccaccgt gatcttgcgc
     1561 gacaggtcgc cattggctac tgcaatggtc acttccgaga tgttgcgaac ctgcccggtg
     1621 aggttgctcg ccatcgagtt cacgctgtcc gtgaggtcct tccaggtgcc ggcgacgccg
     1681 gggaccgccg cctgaccgcc cagcttgcct tcggtcccca cttcgcgcgc cacgcgcgtc
     1741 acttcggaag tgaaagcact catctgctcg atcatcgagt tcacgatggt cgccgaccgg
     1801 aggaactcgc ccttcagcgc ccggccgtcc acctcgagac tcatcgcctg ctgcaaatcg
     1861 cccttcgcca cggcggtgat cgttcgggtc acctcggcgg tcggccagac cagatcgtcg
     1921 atcagggtat tgaccgagat ctccatggca ccccaagcgc cgctgcgggt atcggtggac
     1981 atgcgatggc gtgtcttgcc gtccttgccc acgatctgcc cggctcgctc cagttcgcga
     2041 gccagccgct ggttggagct gacgatctcg ttgaaggtat cggcgacctt gccggcgatg
     2101 ccggtctggt cacccgcgag gcggacggag aagtcgccat cgcgcaccgc ctgcagcgcc
     2161 cgcaggagct tgcgcaggtc gaggctggcc ggctcggtag cccccttgcc attggtcttg
     2221 gaaggacgac tcgctcgctc ttggctttgc atcagcaggc tccaggatgc tcggggtgcc
     2281 cgcaggggtc ccccccaata taagttcgcc gcgcgcgcac tgagcaacac ggcccgaacg
     2341 cggcgcatgg caaagtcccg gcgactgtgg ccgggtagcg aagtgccttg ctgccgaccc
     2401 tgatgcggcc gcgggcacgc ttatccacca ccacggagat tctccagacc cgcccccaaa
     2461 ttgggacggc ctccgtgcga catcgacccc gaaaaatcgc tttattcttc agtctcgtcc
     2521 aacccgggcg accatagcag aagcccatac cggcgcaagc caaatccgtc ccgtccgcgc
     2581 aagccatcac gagcatgcgc tgtcatctgg gtgtggcacg atcaggtcct tgccccgtag
     2641 cgacgctcgg tgcatagttc tgagtgcgaa aatacgtccg gaccgccttg atcgggccga
     2701 gcagaaagcg aacacgatga gtgcctcaac ccttggcgag gaagtggcct ccaccccgga
     2761 gccccgcccc gcccgattga acgtggctgc gttacggcgc gcggtcatga tccggatgtg
     2821 gggcggcaag ggctgcggca gtccttgcga tttctgtcgg gtcgtggtga cctccaccga
     2881 tgtcgaatac gaaatcgatg cccgcctcga cggccagatc gtgatgctgc acttccatcg
     2941 ccgctgctat gacgcctgga aatcaggtca ggaacctgcc gagccggcgc ccgccgaatc
     3001 ctcgccgccc agcgcggctt gacgtgtcgt ccattccgcg gaccggcgcc tgcgccatgc
     3061 ttgtcatcgc ttcctgcgcg ctggcagcca gcccgccccc gccagcggcg cccgctgagc
     3121 cccaggaatt ccaggtagcc cactccgccc tgctcgccat cgaggcggca ccgacggatg
     3181 acagcgtggc cctgtatatc aagcacgtcg ccgatcagac cgcagtgaac agcagcgatg
     3241 tggtcgtcac ggtggatggc aaggcgcaaa ccatctcccg cgccaaggac ggcagctatc
     3301 tcgtctcggc agcggaaatg cgcgataagg gtccgcatac actcgagatc accgtcagcc
     3361 acgatggcat ccgggaaatc ctctcgggtc agctcagcct gcccagtgcc accgaaggca
     3421 gcttcatcag cgcgcatcgg cagatcggct ggtggattct caacatcggt gtggtgctca
     3481 tcgccgcgat cgctttgtcg cgccgcagga agtaattccg ccggtgcagc gaagccggga
     3541 tgcccggccg gcgacgctgc cggaccggac gaactcgaca ctctcggacg ggatagctgc
     3601 aagggcacta acgctctggt agcgtgaggg cttcggtgtg gctgcctatc gctgcatgcg
     3661 cctccgcaga ctccacatat ctataggtat ctcgctgctg ctcctgctgg cgcagcacgg
     3721 tgccatcgtc cacgagttga gccacatctg ccgggtcggc aatgtggaag tgcgtctgca
     3781 gcccgacacg gcgctggaga agacctgcga gctctgcctc gcgtactcgc agctcgccaa
     3841 cccggccggc cataccgtcc acgccctggt cttcgagccg gctgcccatg cagtcggcac
     3901 cgaccgtccc tctgcggcga tccccgccga agtccccacc gcgcgcagcc gcggcccccc
     3961 gctcctgagc tgaatccgtc aattccccgg tccgcttgcg ccgcatgaac ggcggaagcg
     4021 gccattcagc tttcgtggag tcgtttcatg acccgagcac tctttcatgg aggtgcggcg
     4081 tccggccgca ctgccgccct ctgcgcgctc ctcctgagcg cgcccaccct cgcgcaggtc
     4141 gagcaacagg ccagcgcagg gatcccgacc gttgtcgtca ccgcccaaca tctgaatgag
     4201 gagcggtcgc gcatcgacac cgacaccggc gcatcaacct ataccttcga ttccaaggtg
     4261 atccagtccc agccgggcgg cgagaaccag cagctcaacc aggtcatgct gcaagtgccc
     4321 gatgccgccc aggactcctt cggtcagctg cacatccggg gcgatcacaa cgggctgcag
     4381 ttcagactga acggaatcat tctgcccgac ggcatcagct tcttcggcca gaccttgccc
     4441 ccgcggctga tcgcctcgat gaagctcctc accggcagcc tgccggccga gtacggcctg
     4501 cgcagcgccg gcatcatcga tctcacgacc aagagcggcg cgctgcagcc cggcggcgag
     4561 gtctcgatct acgggggcag ccatgactcg ctcgagccga gcctcttcta tggcggctcg
     4621 tccggattga cgacgtactt tgtgaccgga gacttcctgc gcaacgacct cggcatcgag
     4681 tcccccgatg gctcctcgga cccgctgcac gatcacacga agcagtatca cggcttcggc
     4741 tacttcgagc agatcctgag tagcagcgac cgtatttcgg cggtcatcgg cacctcgcac
     4801 gacaagttcc agatcccgaa tctgcatggc gtgcatgcga tggatatcgg tccgggcctc
     4861 aacgtgaacg gcgagaccga ctttctcagc aacgacctgg atgagaacca gaccgagatc
     4921 gatgcgttcg ggatcctgag tctgcagcac tcgcggggca acctcagcgt gcagacctcc
     4981 ttcatcgccc gctattcgag cctcaggttc tttccggacc ccaatctggg cgacctgctc
     5041 ttcgatggga tctcgcaaag cgcctacaag cgcgacctcg ccgacggctt gcagagcgat
     5101 gcggcctacc tgctgggcga tgcccatacg cttcgcatcg gtctgtacgt gcagcgggat
     5161 cggctcacga gcaatacgag ctcacaggtg atccgcgtgg atgcgagcgg cgcgcagacg
     5221 agcatcatcc cgctcggcat cacggacgat atcggtgcaa cgcagtggat cgagagcggt
     5281 tatatgcagg atgaatggcg tctcgcccag tcgctgacgg tcaactttgg gctgcgctat
     5341 gaccattacc gagccttcag tagcggcagc cagctcagcc cacggatcaa tttcgtgtgg
     5401 caggcgctgc agaacacgac ggtgcacgca ggctactcgc gcttcttctc cccgcccccc
     5461 ttcgagctgg tcgggggcga gactctcgac agatttctga atacctcagc cgcccccgcc
     5521 gtgagccgcg atgatccggt gaccgcggaa cggtccaact actacgattt cggtgtgcag
     5581 cagaagatca gcccgatact cacgctgggc gtggacagct attacaagca ggcgaccgat
     5641 ctcatcgatg agggccaatt cggcgctccg atcatcctga ccccgttcaa ctatgctcat
     5701 gggcaggtct atggagttga attcaccggc caggtcacga ccgggcagct cgaagcgtat
     5761 ggcaacttcg cgatccagcg tgcgatcggc aaggacatcg tctcgagcca gttcaacttc
     5821 agcgcggacg acctcctcta catcggcgat cactatattc atctggacca tgagcaacag
     5881 gtgacagcct ccggcggagc cgcctacagc tgggcgggga cgcgcctcag cggcgatctg
     5941 ctgctggggt cgggattacg ctcggatctg ctgttgccgg agggctcgag cattcccaac
     6001 ggcgaccatc tgccgtacta cacccagatc aatctggggg tgagtcatga tttcacgcgg
     6061 gcgggggttc gggggctgag cttgcgtttc gacgtgatca acgcgctcga caggatctat
     6121 cagatccgca acggcacggg ggtgggtgtc ggggcgccgc aattcgggcc ccgtcgaggc
     6181 tttttcttcg gcgtatcgca ggccttctga gtcggcttgg ggctgcaggg acgcagcttg
     6241 ccaccccgct tcagtcgatc gagcgggtcg caacccacag tgcgagctcc tcggaggaca
     6301 cggcgggact gtgcagatag ccctgagact gctcacaacc cagggcgtcc agggtcttca
     6361 gctgttcgat gttctccacg ccttcggcca ccgttcgcag tccgtagctt ctcgccagtg
     6421 cgaggattgc gatgaggatt tcgtggctat ggggctcgga agtgaggcgg ctgatgtacg
     6481 cgcggtcgat tttcaggctg tccaccggca gctcgctcag ccggcagagt gaggattggc
     6541 tcatcccgaa gttgtcgatg gcaatccgca caccctcagc gcgcagcgct ctgagcgttt
     6601 gcctcaaaac ctgcagatcc atgcgcaagg ctccctccgg gacctcgata tcgatgcaag
     6661 gcagcgtgtg cgcacggcgc gtcttgtcga gaaagaaagc cgcgaaatcc ttccggctga
     6721 gctcgggtgc agagatgttg accgccacgc gcagcctggg gagcccggtg ctgtgccagc
     6781 gctcgaagtc gctcgcaact tgctcgagca cccacgcgcc cacttcgcac atcagtcccg
     6841 cccgcgcgag caccggaaga aagacgtggg gtgccacgag tcccagctgc gcgtcccgcc
     6901 agcgcagcaa ggcctcgacg gcggtcactt tgccgctgac gcgctcgatg atcggctgat
     6961 agttgaggaa gaagctctgt tcctcgagtg cgacccgcag gcgcttctcc aggattccgc
     7021 gtcgcgtgtc gctgacagca gaggcagggg ccacgcgtga ggtcggctcc tgtcgatctt
     7081 tgatcgtctt cagcgccgcc tcggcgtgct gcaggagcgt ttccgcgtcc tcaccgtcca
     7141 ccggatagcg cgcgagcccg cacttcaggg tggcctgcac cggcccgtct gcgagtaaga
     7201 actgctgtcc gaaaatggcc gtggcggagt cgcgtgttgg acccaggtga ttgcggcgct
     7261 cagcaaacac agcggcgaag gtgccacccc cgaagtgagc gagatcggcc ccttgcccga
     7321 agcgacgttg cagccgctcg gcgacgcact ggatgagctt gtcgccagct gccggaccgc
     7381 tcgtctcgtt gaaggcctgc agccgttcga tgtcgacgac gatcacggtg gtgagcccct
     7441 ggtcctgtgc ccgctgcagc tggtgcgaga gatgctccgt aaagaaggcg cgccggggaa
     7501 gcccggtcag gggatcgagc agcgaggggt tgagctctgt cggcgcgtcg ttccgccttc
     7561 tgctacggcg atcgccgttg ccgagcacgc gcgtgacgga gtcaatgagg cgaggcagct
     7621 cgcttttggg gacatagtct gatgcgcccc gactgagcgc ctcggcggcg cgctgctcac
     7681 cgatggtgcc ggagacgaag atgaagggca cgtcgggcgc ttcggcggcc gcgagctcca
     7741 aagcggcgag tccgttgtac tgcggcagcg tgaaatccga caggatcagg tcgggggcga
     7801 attcccgcag ctctgtgcgg aattcctctt cggtctccac gcggcgccag gtgcacgagt
     7861 agccgccgcg ggtgatctgg ctggcgctca gctccgcgtc cggctggcga tcatcgacga
     7921 tcagcaggcg taggggatgc tccaagatag ccccgggtgc aactccttgt aagcgcatgg
     7981 tgcgcccagg gaggaaagcc ggaaggtgcg ctgtttctca gttccgggac gtgctccggc
     8041 gcaatccctt gcgggcctgg tgccagagcc tccgtaagcc ccgctccggt gccccgactg
     8101 tgaaacctgg cccgcaggcc ctggccgacc atccccctcc gcgggccgcg acccggcagg
     8161 aattgtgtga actgtgacct gaggcggcct gtgccgagtg tgacgtgtat aggctcggcg
     8221 caccttcctg cctgagcggt ggactcacat gtctagttcg ttgactggct cctctcgcag
     8281 cttcgtcgtg ccctgcgctg gattgctggc gctcctgggc accctcctcg tgagcggttg
     8341 tgttgtgacc actgcgccgc cgccgcgcgt gtatgcgccg cctccgcctc cacctcccgc
     8401 cgctgaatat tcagcaccgc agtcggacga ggaagcggtg cagactgctg aagcaccgcc
     8461 cccgctgccg gactacgaac agccgccttg ccctgaagag ggctacctct ggacgccagg
     8521 gtattggcgc tggagtcccg ccggctactt ctgggtaccg ggaacctggg tcgctccacc
     8581 ccgtgtcggt gtcctgtgga cccccggata ctggggcttc gcctccggtg tctacgtgtt
     8641 tcatcctgga tattggggtc cgcacgtggg cttctatggc ggtgtcaact atggatacgg
     8701 ctatggtggc gtgggcttcg taggcgggcg ctggtccggt gcccggttca cctacaacac
     8761 gagcatcacg cacgtgaata cgacggtgat tcacaacacc tacaacgaga ccgtcgtgaa
     8821 taacgtcacc atcaacaagg tgagttataa cggcggggcg ggcggcattc gggctatgcc
     8881 cactgcccag gagcgaatgg cggcgcagga gacgcacgtg cccgccaccg cgtcccagca
     8941 gcaacatgtg caggaagcgg cccgcaatcc ggggttgtcc gcgaaagtca acggcggcca
     9001 tccttcgatc gccgcgacgc cgcggccggg cgccttcaat gctccgggtg tggcgcgagc
     9061 ccatggcgca cccgccgggc agatggcgcg accggcgcct gggaagcccg ccggcgcaaa
     9121 ccccccaggt aaagcacctg ccaacaaccc tgccctggca cgccgcttca ataacacggc
     9181 acagccggct tcgagccatg gcggtcaacc ggcgcatgcc acgcaggctg cccgggccca
     9241 accgcagaag aacgcacgcg agaagaagtc tccgcaggag cgcaaacagg agtccggcgg
     9301 cgccgccggt aactcgcgct gagttgaacc ggcctggcgc cgcagggcgc caggccaccg
     9361 cccagcaatc cgcagccccc gcgcccgcag cgctatgtct gcggaggccg aacgctattg
     9421 tctatgctcc tcgtgaggag cctcgcaaag gatgccgacg aggctccgcc acccgttggt
     9481 gaggagccgc ttgtcgagcc tggcgaaccg cttcgatcgc atggaatggg ccggtgcctt
     9541 cggcgatctc ggtaccttcc tgccgttcgt cattgcctac atcagcgtcg tgcgggtgcc
     9601 ccccgccggc atcctgcttt ccttcggcct cgcgctggtg ctgtgcgggc tgtattaccg
     9661 aacaccattt ccggtgcagc ccatgaaggg catcggcgcg atcgcggcaa cgcaagctgc
     9721 gaaaagctcc ttgatcacac cggcaactct ctacgctgcg gacctcgtga ccggcttgat
     9781 ctggctcgtt ctgggcctga ccggttcggc acggtatgtc gcgcgctggg ttcccgggat
     9841 cgtggtttcg ggaatcgtgc tgggactcgg catgagcttc atgctcgagg gcattcgcat
     9901 gatgagcgcc gactggctca ttgcgagcat cggcttggcc ggcaccctgc ttttgctggc
     9961 cagccgcgcg atccccgtca tgttcctgct gctcctgttc ggcgcagtgt gcacgctcat
    10021 ccagaatccg tctgccatga gcgcgctatc gagcgtgaga ttcgcgctgc atgcgccagc
    10081 cttcgccctg gcaagcatca gctggcgtga cctccttgca gctacggctc ttttggcctt
    10141 gccgcagctg ccactcacgc tgggcaacgc tctgatcgcc acgcgcgagg agaacaaccg
    10201 gctgtttccc gagcggcccg tgagcgagag ctcgattgcc acctcgaccg gcctcatgaa
    10261 tctgaccagt agtgtcttgg gcggcgtgcc aatgtgtcac ggcgtcggcg gcctggccgg
    10321 acaagtcacc ttcggcgccc gaaccggcgg ggcacccatc atattcggca ccctgttgct
    10381 cgtcctggca ctgggtttca gtgatgccgt tcagagtctg ttcaacattt ttccgcacgc
    10441 cgtgctcggc gtgattcttt tcatcgcagg cgggcagctg gcactcggct gcggagagct
    10501 gagcaggagc aaggtcgagc gtttcgtcac gttcatcacc gctgccttca ccgtctggaa
    10561 cgtcggcgtc gccttcctgg tcggcatcgc gctcacatat cttgcgggac gcggacggct
    10621 gcggttgtag cgttgaccct agtccagctg ccagcgaacc cccgcgaaca gctcgttggc
    10681 caccttttcg tccggctcat tgacgagcgt gaacttcagc cagcgatacc cacctagcag
    10741 gaaaagacga ttgctgacct tcatctccgc gcctgcggtg aaatcgtaca tgttgtgtgc
    10801 cgagccgaag gtgagtacgc cgggtgagta gaacgcgctt ccgaacgctg acagccccaa
    10861 acgccggatc aactcgtagc gagcgttcac gccgacagag gcggcgaaga cgtcggtctt
    10921 ctgcgcagcg ttcagccacg cgaggttcag ctcaggaccc acctgcactc gcaggcgcga
    10981 cagaggcacg atgtcggtat cgaacatcat cgccgccgag ccgatgaatt cgcggtcctc
    11041 ggtgagcagc gcgccgaagt ccatattgcc ggcgagaccg gcgaacggcg atccggtcag
    11101 atatctcatc tgcagcactt ggtttccgac tacggcatcc actgcggagc cggtgctttg
    11161 cgccaggggt gtctgtgcca ccgctggagt gatcgcagca gcccccattc cgaccatcat
    11221 tactacaatt agccaagctc tcatgagatt cctttttagc agcacaccat ggccgctgtg
    11281 ggctcgccat gcagcaagcg agagtcctgt tgcgagctcg atacaacacc cgacgtcggc
    11341 gaagcagaca cgcgaccccg cgcgctcgca ggatgcgggc gtgagcacgg ctgtgccgcc
    11401 aaggctattg ccgccgggcg gtgccgggct cagcttcctt gcgatgttgc tcggctagca
    11461 ccgacgaggg cgtgacggtg gccactgccg actgcacctt gttcttgaag ccggcgacca
    11521 catcgccctc gccgtccatc atggccttga agccgatccg ggctacaccg gccgcgtcat
    11581 ctttcttcga gctgccgatc ttcgtgtcca tcatgccggc acgctggaag aactccgttt
    11641 cggttgctcc aggcatcaag caggtcacgg tgacgcccga gtctttcagc tcattacgca
    11701 atgcccagga gaatgagtcg acgaaagctt tggtcccgtt gtacaccgcc tggaaagtcc
    11761 cgggcatgaa gcccgcgatc gaaccggtga tcaggatgcg acctgcgcct cgggcgcgca
    11821 tccggttgcc cacggcatgg atcaacgcca gggtaccggt gatgttggtg tccacgacat
    11881 gttgagcctc ctcgaggggc tggtcgagga aggcgccacc cagtccgtgc cccgcattcg
    11941 ccagcagggc atccacggga cggtcagcga gacgatcgag gagcgcctga acgccctccc
    12001 gggttgccag atcggcctgc agcgagtccg cttgcgtgcc cagctcgcgc atggccgcca
    12061 cggcttcggt cagggggcgg tccgcggcca gaaagagatc gaagccattg ctggcgcatt
    12121 cacgtgccag ctcgaggccg ataccgctgg aagctccggt gacgacggcc aggggtcgaa
    12181 tcgcgcttgt cataaatcct cacatgttga agtttgaaga cgatgcccga tcaggtagcc
    12241 gattgatgca gcacgaaacg aacctcgtga aggtggcgct actggaggtt gcgcgctcga
    12301 gatggcccgc tcgcgcgttc gagacgggag ctcgtgcgcc tcgctcgcag caagctcgag
    12361 ctggatctgc aggtgctcag aggcgccccg ccatggaaca tcagggagcg tgcggaatta
    12421 ctcagccagc gcgtcttcac ggcggcggtg ggtaccgtct ggcttatggc atgaagagga
    12481 tcgaggcggt cggtgagccg attgaagggc tgagcgcggc ggcctatcgc gtgccgacgg
    12541 atcgacctga agccgatggc accttcgcat ggaactcgac cacgctgatc ctcgtcaccg
    12601 cccgggccgg gggccagtca ggcctcggat acacctacgc cgatgcctgt attgccccgc
    12661 tcatcaccac cgtgctggcc caggccattg cacagaaaaa cagcttcgac atcgccggct
    12721 gctggtgggc catgcaacgt gcggtgcgca atctcggccg tgaggggcta gccgcctgtg
    12781 ccatctcggc ggtcgatgcg gcgctgtggg atctgaaggc gcgcagcctg gggcttgcgc
    12841 tggccagcct gctgggtcgt tgccgcgaag cggttcctgt ttacgggagc ggaggcttta
    12901 caacctattc ggacgatgag ctgcgcgagc agctttccgg gtgggtggag caaaacggct
    12961 gccgagccgt gaagatgaag atcggcagcg agcccgctcg cgatccccac cgggtcacgg
    13021 tcgccaggga agccatcggc gagcgcgagc tgtttgtgga cgcgaacggg gcactgtcgg
    13081 ccaaagaggc gctgcgattc gtacgcgagt gcccggaagc gagtctgcga tggttcgagg
    13141 agccggtcag ctctgacgat gtcgcgggtc tgcatctgat ccgcgagcgg cttcccgctg
    13201 gaatcgacgt ggccgccggt gagtacatct acacgctcga tggcgccgag cacctcctgg
    13261 caggcgatgc agtggacgtc ctgcaggccg atgtcacccg ttgtggaggc attaccggat
    13321 ttctcgccat cgggtggctt tgcgagagcc gccatatcga cctgtcgggc cactgcgcgc
    13381 cttcgcttca tcgccatgtt gcctgcgcag ttccgcgctt gcgccatctc gagtggttcc
    13441 atgaccatgt ccgcatcgag cagatactgt ttgatggggc tccggtcgcc gcagacggcg
    13501 tgatccggcc ggacctgagc cggcccgggc atgggctcga gttcaaggaa caggatgccc
    13561 gtcggttcgc gatccaggca tcgggctcat gaggcagcgg cgccccgtga tgcgatcgcg
    13621 atctcccgca tccgaggtgc cggtccgcag cgcccgacag ctcaacctgg gctcggcgat
    13681 cctggctctc tccgtgctgg ccgacagtgg gatagagcac tatcgaggct cgttccacaa
    13741 tcgtgcgatg tacacaccgc tgggtgttgc gagcctcacg cttgcggtca gtctgttagg
    13801 tgcactcgat acccggccgc gtggacaccg tgcacgggat gcgatttatg cactttccgc
    13861 cgcgaccggt ctcgcaggct tcggcttcca cctctataac gtcgccaagc gacccggcgg
    13921 cttctcctgg ctcaacttct tctacgcggc gcccattggc gccccgtttg cactggttct
    13981 ggccggatta ctcggcaagg gcgccgagtc ggcccgcagc acgagggcgc cctcgccagg
    14041 cgcccgtcct tcgcaagccg cccacttctg cacccaccag gccggacgag tgctcgcagc
    14101 gttgagcgca gtcgggttgg ccggcaccgt ggccgaagcc gcattgctgc attttcgcgg
    14161 agcgttccag aatccggcca tggtgcttcc ggttagcgtg ccgccggtcg ccgctgctct
    14221 gctggcggca gccgccgtga gatccactcc ctccgcgatg cggcctgggc tcgtgtgtcc
    14281 tcgtggcgtg cgtccgcccg gcacgcagcc gggccgccga tcgcgtggcc tggcgcgctg
    14341 gtggctgaag ttgaccgcgc tcctgggctt tgtcggagtt ggttttcatg cgcgcggtat
    14401 cgcacgcaat atgggcggct gggggaactg gagccagaac atcctcaatg gtccgcccct
    14461 gccggctccg cccagcttta ccggcctggc cctggcgggc ctcgccgcgc tcggattgat
    14521 ggaggacaac gtggatgagt gatcgctatc ccggctatga cgtcctcgcc aagcgggact
    14581 ccccttcctg gaacgcccag actcgcacgg tcgtggatgc tcggctggcc gtgcagccgg
    14641 acgcacacac ctttttctcg caagccgaat ggccctcagt gtgcgccatc tgcgaccgca
    14701 tcatccccca gcccccgcag gcatcccggc gcgtaccgat tgcggctctt ctggatgcgc
    14761 acctgtgcaa catcgcctcc gatggctatc gggacgctcg aatgcctccc atgcgggagg
    14821 cgtggcggct cgggctgcgc gcgctggatg ccgagtcgca gcgcgcgcat gggcatcgct
    14881 tccatgagct ggaggttact cagcagtccg ctctgctgcg agtcgttcaa caaggccata
    14941 cgacggaccc ggcttggcag agcctgccgc cggatctgtt cttcagcaaa cgggtgctgc
    15001 acgacatcac ccagctttac tacgctcacc cttacgcctg gaatcagatc ggcttcggcg
    15061 gcccggccgc tccgcgcggc tatgtgcgta tggacttcga tcgccgcgac ccctgggaag
    15121 cttccgaggc gcggccggga cgcgaagaag aagcgcggcg cgaaaatgcc cggattggat
    15181 gacgccgctc agagtccgcg ggccgtgaac gggcgcgctc ccgacgtatt tcgggtgggt
    15241 cgctgggtcc cgatgcgcca gtacagcgag gccgagccgg tcgatttcgc catcgtcggc
    15301 accggtgcag gcggtggcac cctcgcatgc aagctcgccg agtatggctt ctccgtagtc
    15361 gccctggacg cagggcccta ctggcgccct ctggaagact tcgcttccga cgagttgcat
    15421 caacgcaagc tttactggac cgatgagcgc atcgtagacg gggagaaccc gctgcagctc
    15481 ggatccaaca attgcggcaa gtccgtgggc ggctcgaccg tgcatttcgc gatggtgtcg
    15541 ctgcgctttc gcccggaatg gttccgatcc cgcacgctgc tgggctacgg ggcggactgg
    15601 ccgctcgact ggcgggagat gtggcgctat tacaccgagg tggagcaggc gctgaagatc
    15661 tccggcccgg tccgttatcc gtggggaccg ccgcggccgc gctatccgta tcggcctcac
    15721 gagctcaatg ctgcggccct cgtgctcgcg aagggcgccg aggcgttggg aatcgattgg
    15781 acggctacgc cgcttgcgac gctatccgct cctcgcgggc ttgctcatcc gtgtgtgtac
    15841 cgcgggatgt gtgtcatcgg ttgcgccacc aacgcgaagc agagtgtgct cgtgacctgg
    15901 ctgccccgcg cactcgcggc cggggctgaa atccgcgatc tcgccatgat cggccgcgtg
    15961 gaccatgatc caaacggccg cgtgacgggc gtgcactact ttcgccaggg cagctggcat
    16021 ttccagcgag cgcgcaatgt cgtcgtcgca ggctatgcca tcgaaacgcc gcgactgctg
    16081 ctcaactccg ccagcagccg ctatcccgat ggactcgcca actcctcggg gctcgtcggc
    16141 aaaaatctca tggtgcagtc gaaccaggcg gtgtggggcg tgatgaaaga ggagatccgc
    16201 tcctacaagg gccctccttc actcgccatc accgagcatt ggaactacgc cgatgagggc
    16261 aaggacttct tcgggggcta tagctacatg agccaggggc cgctgccggt cgtctggtca
    16321 ggaaccgagg ccggcaagcg cggactgtgg ggccagcagc tgctcgatga gatggaaaaa
    16381 tacaaccacc aggtcggtct gaagatcgtc ggcgagtgtc ttcctcagga gcgcaatcgc
    16441 gtgaccctca ccgacctcaa ggatcagtac ggcttgccgg tcccgcgggt cacctactca
    16501 tactgtgaca atgacaaacg actcatccgc cattccctga agttcatgcg caaatcgctg
    16561 gaagcggtgg atgcgaccga gttgtgggat gagaccgatg acacgtgcca tctgaatggg
    16621 accgcgcgga tgggcgccga tgccgcgagc agcgtggtcg atgccgactg ccgctcctgg
    16681 gacattccca acctgtggat ctgcgatggc tcggtatttc cgacagtagg cggcgtgaat
    16741 ccctccctca cgatccaggc aatcgcctgt cgcaccgctg accggatccg cgccctcgcc
    16801 gcccgcggcg agctctgagc ccccggccga attggctcag cggcgactgc gccagcgtcc
    16861 gatacccaca cccagagcga gaaaagcgat cgccgtggcg gcgaacgccc cgagtcgggc
    16921 gatctcaccg accatcagcg gcgcactgtg cgcagcttct gcctcgaagg atccgcgcgt
    16981 gcgatgcagc tccgtgacgg gccgctccag attgtcgcgc cggctgcggc cgatcggctc
    17041 gcgcgtctgt tggccgccga aggccgttct cgccagatag cgatccagga aaccgggcag
    17101 cacggcatcg cccaggatgg tgagcagcgt gggaccgccg agccagtact cgcgccagcg
    17161 cccgcgagcc acgcggaaca cggcatcggc aacgacttcg ggctcgatcg gacgacccat
    17221 cggccgtggc gagtgactga tgtgcgccct cgcccagtcg aactggggtg tattgaccgc
    17281 aggcagctcc acgatgctga tgcgaatcgg cgagccctcg tgcagcagct ccgagcgcag
    17341 cgaatcggta aagccacgaa tcgcgtgctt ggcaccgcaa taggcggatt gcaggggaat
    17401 gccgcgatac gccagcgcag agccgatctg gatgatccgt ccggagcgcc tcgggcgcat
    17461 cgacttcaaa gccgccatcg tgccgtgaac gaaaccgagg taagtcacct ccgtgacgcg
    17521 gcggaactcc tcgggcgtga tgtccgcgac agtggaaaac accgtgacca tcgcatcatt
    17581 gacccagaca tcgacctttc ccagcctctg ctccaggctt tcagctgcag caaagagcgc
    17641 cgaagcgtcc gccacatcca ccggctcgaa tgccacttcc gcgcctcgcg actgcagttc
    17701 ccgactcagc gcttccagag cgggggcatc ccgagcaatg agcccgacgc ggtcccccgc
    17761 gcgtgcgaag cgctcggcga tggcccgccc gataccagcc gcagctccgg tgatgatcac
    17821 tgtgcgcgcc gtttccgtcc gatcagttga catgatgcct ccgggtcgct cgcgctcaac
    17881 cttgaacggg ataggccact gtgctgagca gtgcgatgtg ggtatctcgg gtgtcgagcc
    17941 gggtgtgttg caccacgacc ggcacatccg agcggatgat ggatgagtag gccgtgtcgc
    18001 gcggcacccg ctccggctcc tgcaggtcgt tgaatcgcag gtgcagggtg cgttccgagg
    18061 gcaccttcac acggtaagga cctgcgggtc cgcgatcctc gaagtacaag gtgatctcga
    18121 tgttggcatc cgtgtccgcc gcattcagga tgcaggcggt ctcatgagac tgcagctcgc
    18181 gcgattcgct cacgctgcgt cccggaatgt agccttccgc gattgcccag gtccgatgtc
    18241 cgatcatttg cgtgctccgc caagacaggc cgtgaattcc gcgccgaaca ggaagatcag
    18301 cgaggtgtag tacaaccaga gcagcagtgc cgcgaaagag gacgcggcac cgtaagccga
    18361 gggttgcgtg gagtgggcca ggtacaaccc tatggcccat cgtccgatat cgaacagaac
    18421 tgcggtgagc aggcccccga gcgcgacatg acgccaggcc agccggcgcg cgggcatgta
    18481 ccggtagatc aaagcaaaca gacctgacac caccgtcagt gagacgagaa tatcgagcac
    18541 ggcgatggcg ccgaccatcg ccggatgcgc cgcggccacg cgatcgcgta ggttggaaag
    18601 gccggtggag atcgtcagcg atatcagcag caggaatccg agcgtcagaa tgaacccgaa
    18661 cgacagcagg cgcgtccgga accagttgta gagaccactc ctgacgagga ggtcgctctg
    18721 ccagatgtgc tcgagcgcct gctgcagcgc ggccagcacg gtggtggccc cgatcatcag
    18781 ggtcacgacg ctgatgatcg tggcaatgag cccgcgagtt tgctccgcac tgtgcacggc
    18841 gccgagcagg atctttgccg tggaggggcc gaagagcgcc cccagctgca tgccgacctc
    18901 gccgtaagcg gacttcgcac cgaggaagaa cccggcaatg gtcagcagaa tcaccagcag
    18961 cggagccagc gagaatgcgc agaagaaggc cagcgccgcg ccgatgttcg agctgttgtc
    19021 cgagttccag taacgcgccg cgcaggtggt cagactccac aggtgcgcca atctctgcct
    19081 cagggtgacc atgctcctct cgccgggagg gcagatctcc tttccccatg gggctgcggc
    19141 ctcgcactgc gtgggcttcg gtgaagcaag aaccacgccc gtagcggtgc agggggcgcg
    19201 gatagcggtc cgtttgccgg gcggccggat gtctggttgg cacccccgtc ctgtctagca
    19261 ccggatcccg ctttgccgca gatgcgagag cagctccccg cgcagccgct ccagtccatc
    19321 cggatcggct gcctcacagc gggcagtgag cttcggctcc gtacccgagg cccgcagcag
    19381 ccaccaacct tcatcggctg tcacgcgtaa tccatccgtg gtgtccacct tcgccccccg
    19441 tgccacaagc cgcgccgcga cttcgcgaat cgtcgcttgc ttgcgcgatt ccgggcaggc
    19501 cacgcgcagc tcgggggtcg ccgacattct gggcaagctt tcgcgaaact cggccagcgt
    19561 acatccttgg ttgccaagcg ccgccagcag tcgcatggca acgtacagcg catcatcgct
    19621 cgcatgccag caatcggcat agacgatgtg cccactcatc tccccggcca agggagcttt
    19681 ctcacgcaac atcgcttcgc ggacgatgac gtatccggaa ggtaccatca ccgcacggcc
    19741 gccgagccgc tcgacctcgt cgaacagcac acggctcgac ttgacgtccg caacaactgt
    19801 cgcgcccgga tgtgcagcaa gaatctctag cgccaggaac aacacgagct gatcgggcca
    19861 tatgatgcga cccgtggcgt ccaccacacc gattcgatca ccgtcgccat cgaatgcaat
    19921 accgagatca gcacccttcg caacgaccat cgtttgcagc tgtcgcaggt ttgccggcac
    19981 agcggggtcg gggtgatgcg ccgggaagct gccatcgacg cgcgcattga gcagcgtgtg
    20041 ctgtccgggc agccgcgcca cgagcgcacc gatcacggtc cccaccgcac cgtggccaca
    20101 atcccagacg acgtgaagct cgccagcacc cgctgccacg gcggcaagtc gtcgcacgta
    20161 atgcgttgtg atctcgatcc cgccgagata taaatcgtgt gtcgcaccac cgggccgctt
    20221 tgtggcgggc gtagcaacca gacgcttcag cgccgcgcca aacaccggct cgccatcgag
    20281 aaccaacttg aagccgttct gctccggcgg attatgcgag cccgtcacca tcaccccgcc
    20341 ctcgagctcc gcggcctgta cggcgaagta catccgtggc gtgggtccga ccccaagtcg
    20401 cagcacattc attccacctg cagtcaatcc tgcggccaaa gccgcctcga gatccggcga
    20461 gctgagccga ccgtcgcgcg agacggcaat gcggtgcttt ccgcgggcga tggcgtgagc
    20521 ggcgtaagcc aggccgagcg catgagcgtc agcgaggccc aactgctgcc cgacgacgcc
    20581 gcggatatcg taggagcgga tgatcgcggg attgattgat ggagctttgg gagagcgcac
    20641 ccagggcaaa cgggtgcgct acgcgtcagt tccaacatca tctgggcgtt acgggcgcgc
    20701 cccttgctga gacgttacgg tgaagatcgt catctcaccc ggagcctttt gccatgagcc
    20761 ctcaagttga atcgcccaaa cccgagttca agtcacaacg gcagccgatc ccaggcaaga
    20821 ccgaagacat ggagccgagg cctgaccacg gcgagtcaag ctatcgcggc tcgggccgtc
    20881 tcgagggcaa gaaggcgctg atcaccggcg cagattcggg aatcggccgt gcggtggcca
    20941 ttgctttcgg ccgcgaaggt gcagacgtgg tggtcgccta tctcgacagt agcgaagacg
    21001 cagacgcgcg cgagacgcag cgtctggtcc gcgacgccgg ccgcacgtgc gagctgttcc
    21061 gcggcgacct cgccagggcc gacacctgcc gtgagctcgt tcgatgcgcg gtagcatcct
    21121 tgggtcgcat cgatgtactg gtcaacaacg ccgcgcacca gaagagcttc aagtcgctgg
    21181 aagagatacc cgatgaggaa tgggataaga cctttgccat caatatctcg gcgatgtttc
    21241 acatcgtcaa ggcagccctg ccgcacatga gatccggcgg ctcgatcatc aataccagct
    21301 ccatcaatct ggataagccg agcccgcaac tactccccta cgcgacgacc aagggagcca
    21361 ttcagacctt caccgcggga ctcgcacagc tactcgccga gcgaggcatc cgcgccaacg
    21421 cggtggcgcc cggccccgta tggacacctt tgattccctc gaccatgcct caggagaagg
    21481 tcaagcactt cggcgagcag gtgccgatga agcgccccgc gcagccccgc gagcttgctc
    21541 cggtgtatgt gatgctcgcc agcgatgaag ccagctacgt ctccggagca acgatcgccg
    21601 tgactggcgg caagccgatt atctgagtcg gcggcgcgag cggccggcgg ccgcatcgag
    21661 cgatcgccac gagccgctcc gggagtgagc tgcgcaggcg acgatcaccc gaacacgact
    21721 actgtttagc tgagcaccct gttggccggt gaggtctcgg tccattcgcc gttatcccgc
    21781 ccgccgttgt gaccgccgtg gtcgcgcccg ccgccggtct tgcccggctt cagcacgacc
    21841 ttgatgcatt cgtcctgctt atcgcgaaac atcttgtaag ctcgcggcgc ctcgtccagc
    21901 gcaagccggt gcgacacgac aaaactcggg tcgatacgcc cctcctcgat gagctgcagc
    21961 agtggcctaa ggtagcgctg cacgtgcgtc tggccggatt tgagggtcag cgcccggttg
    22021 acgatcgcgc ccaccgggac cttgtccaga aatccgccat aaacgcccgc gagcgagacg
    22081 gtacccccgt tgcgacacgc cagaatcgcc tcgcgcagcg cgctgggccg gtcggtcgcc
    22141 aatctaagcg ccttcttgac ccgatcgtaa gcgccaaaaa agcccgcggc gtgcgcctcc
    22201 attcccacgg cgtcaatgca cgcatcaggg ccacggccgc cggtcatctc acgaagcgcc
    22261 tccggcacgc tcacgcgctc gtagttgagc gtctcggcct tgcagtcctg gcgcgctttg
    22321 cgcagtcgct cctcgaagcg gtcgatcgcg atgacgcgct cggcgccgag gatgaatgcg
    22381 ctggcgatcg caaacaggcc cacggggcca cagccccaca cggcgatcgt gtcaccgggc
    22441 tttatggcac agttctcggc cgccatgtag cccgttggaa agatgtccga gagaaacagc
    22501 acctgctcat ccgagaggtg ctccgggacc ttgatgggcc ctacatccgc atacggaatc
    22561 cgggcatatt cagcctggcc acccgcgtaa ccgccgagca tgtgggagta gccgaacaag
    22621 cccgcgggcg agcggcccca cagcttctcc gccatccagg cgttgggatt tgaattttcg
    22681 cacagggaga actcctccga attgcaaaag aaacaattcc cgcatgcgat gggaaacgga
    22741 ttcaccactc gatcgccgac ctgcagattc ttgacgtcgg cgcctacttc tacgacctcg
    22801 cccatgaatt cgtggccgat gatatcgccg cgcatcatgg tgggaatgta gccgtcatag
    22861 atgtgcaggt ccgagccgca gattgccgtc gaggtgatct ggatgatcgc atcgcgtggg
    22921 ttgaggattt ttggatcggg aactcgctct acgcgcagat ccatcgcacc attccagcag
    22981 ttggctttca tgcccgtacc tcctgttgca tcgagcgctc ttgggttttg cggggcggcg
    23041 gctcacgaag ccgccctgca ccgggcaacc agcggctcag tacacttcgc cgtcctgcgg
    23101 gctgaccggc ggtagtggga atctctccgg tttccaggag ctgcttgaac ctgcgcaggt
    23161 tttcacggat ctctccaaag ggatccgggc ccagaacctg ggcgaggatc gagccgagcg
    23221 ctccaccggg agcacggtag tgcatgctga ctttgacgaa cgtgccgcgg ttgcccggtg
    23281 ccttgtcgaa gtgcaccgca cccgcgtgct gaaacggcgc accggagagg gtgcgccact
    23341 ggatacgctc attcggtacg tcggaggtga tctcggcgtc ccactccatt cgacggttgc
    23401 ccgtcaagcg cagactccag tgagagcgct tgctgtccag gtgcgtgacc gactccagac
    23461 ggggcgagaa ccgcgtcatg ttgcggaact ctctccagaa ggcgtagcac tcctcctgcg
    23521 acttgttcac caggatcgtg cgctcgagat agacatcggg cgaggcgccc ctcgcagcct
    23581 tgaatgggga ggttgtgaat gatgtgcgct gcagggcggt cgccagatct gcggcaccga
    23641 tggcgctcac gaccgccaga gtcgcccttc tgcgcctcct gctttgaggc gtagcactgc
    23701 ccgcgctggc gaggaccgcg atatcgatca ggtctccgac cactcgggac cagagccagg
    23761 tcggatcttc gggacgggcg agcagtccgg ctccgctcag cagctcacgc gcaccgatgg
    23821 cccgcagcag gccggtgcga ttctccagcc ctgtccattc gcccagcgtg cgcggcgcga
    23881 gcagcgcgga gaggccgagt ccaatgctga gccatcccag ccccgttgcc gtggactggc
    23941 gctgcagcac tgcaccgacc gcgcggccct tgccttgtgc ccaggataaa ggtgcccgcg
    24001 tgtagccacc cccgccgcga gcccgctcgc gtgctgatgt tggatctgat tccataaacg
    24061 tctccagtgt gcccggccgc atccggccct tcagcatttg atgtgcaggg gagagcaagg
    24121 cgcgcgcccg ctgatcgggc gtgaggccgc acggccaagg cggtccgcgt agctgccgcg
    24181 tgcgccttca cggtcgctcg caggccttga agacagccga tcgggccctc atgttgagca
    24241 cgatctgagc agtgcgtggc cagttctcat tggacctcga gcggcagctc acgcattcct
    24301 atagcggagg cgaaccgaag ggtggttaga tcgagcccct aaatgattag cgcctcgagg
    24361 taacccataa cgcatcagga tccatcgatg agaatcgcac agatatcgcc gctgtatgag
    24421 gcctgcccgc ctgcccttta cgggggcact gaacgtgtcg tcacctacct atgtgatgag
    24481 cttgtggctc tggggcatca ggtgaccctg ttttccgttt ccacctcacg cacccgggcg
    24541 gagctggtgt cggtgcgcga tcagcccctg aggctggatc cggccgtgct caagtctgac
    24601 gtggccgccc atctgcatct gctcgatgag gtccacgcgc gagccgccga attcgacatc
    24661 ctgcacttcc acacggatct gctgcacttc acgatgttcg agcatctggc ccacaagacg
    24721 atcaccacga tccacggacg gctcgacatg aaagatctcg agcaggtgta tcggcgctgg
    24781 agtcgatttg ggctggtctc gatctccaac gcgcagcgcc agcatctgaa gggcgcgcac
    24841 tggatcgcca atgtgccgca tggcatcccg cggaccttgt gctggccgaa cttcgatgtc
    24901 ccgcagggtt atctggcgta cctgggccgc atctcggcag aaaagcggcc ccacctcgcg
    24961 atcgagctcg ccctgagggc gcgcgtgcca ctgcggatgg ccgccaaggt cgacaaggcc
    25021 gatgcggcgt atttccatga atccgtggag cctctgctgg gccgtcccgg tgtggagttc
    25081 gtgggcgaaa tcggcgacgc tcacaagagc gcattccttg gcggagcgcg cgccttgctg
    25141 tttccgatcg actggccgga gcccttcgga ctggtgatga tcgaggcaat ggcctgcggc
    25201 acgccggtca tcgcctggaa ccggggctcc gttgccgaag tcatcgagga tggcatcacc
    25261 gggttcatcg tcgagaccga ggaagaggcg ctggcggcaa ttgcccgcgc cgacaagctc
    25321 gaccggcgca ccatccgagc acgattcgag caacgattca gttcgcacat catggcgctc
    25381 gcttattctg acgtgtatac gcgttcgctc agtgccgtgc cgcggctgcc cgcgttgcgc
    25441 ccggtgggga cgatcgcctc gtgaagtccg cggacgagac tgtcgagccg caggggctgc
    25501 tgccccgttt cacgcttcgc gacgcggaca ctttcctgct cgccgatgcc ctgggggata
    25561 tccaaggcgg ccgccatgac ggccttttca cttccgacac gcgcttcctg tcccgcttcg
    25621 agctgcaggt gggcgaacgg cgcccttccc tgctgggcgc agcgctttca caggacagta
    25681 cgctgttcac tgcgcatctc accaacctcc ccctgccagc cttgggcgag ggttccgtcc
    25741 cgaagggcgt cattcacgtt gagcgcagcc gcacgctgtc gcgcagccgg gtgttcgagc
    25801 gattgcgcct caaaaacttc agcgaacgtg cagcccaggt gccgatcggg ttcacgctgt
    25861 ccgcggactt tgccgatatc ttcgaggtgc agggcgcgac ccgaaaagag cgaggcgtcc
    25921 tgctccccac ccgacgcacc gccaatcagc ttgcgctggg ctatcgggga ctcgatgacc
    25981 gcgagcgctg gacctacatt tcgttctctc caaaaccggc cgagtggagc cccgagggtg
    26041 cgaagtttgt attcgccttg caaccggctg ccgcggtaga gctcttcgtc gaaatcggct
    26101 ctgaagagca agcggcgccc tcagccgagc gattcaaaca agcctgcgag gcatccgaga
    26161 aaaggatgca ggagcgcgca ggcatcgctg cgcacatcag cacgagcttt cgcctgttta
    26221 gtcagtggac gcagaaatcg cgctttgatc tcgcgctgct gaccacggac ctggccacag
    26281 ggccgtatcc ctacgcgggg gtgccgtggt tcgcgactca gttcggacgt gatgcgatca
    26341 tcacggctct gcagaatctc tgggccgagc cccggcttgc cgagggtgtg ctcagattcc
    26401 tcgcctcgac gcaggcgcac gacacatcgg cttaccgaga cagcgagccc ggaaagatac
    26461 tgcacgagat gcgtcgcggc gaaatggcag cgttgaagga agtgccgttc gcccaatact
    26521 acggcgcagt cgataccaca cccctcttcg tgatgctggc cggtgcgtat gagcgccaga
    26581 cgggtgatcg gcggatcgtc gatgagatct ggtcgaatct gaagaaggcg acagcctgga
    26641 tcgagcgcaa gctcgcagag catgacagcg gatttctgta ctacgcgcgc ggggagcgct
    26701 caggtctggt caatcagggg tggaaggaca gtcaggactc gatctttcac gaggatggct
    26761 cctttccgac cggggcagtt gccgtggtcg aagtgcaggg ctatgcatat tcggccctcg
    26821 cggcgatggc gacccttgcc cgagcgcggg gcgaggacaa gcaggccgcc cactggtctg
    26881 agcgggcaga gacgctgcgc gcggcgatcg agcagaccta ctggatgccc gagcacgggt
    26941 tctatgccat tgcgctcgat gcgggaaccg actcggtctg tcgcgtcgct gcctcgaacg
    27001 ctggacatct gctttactgt ggggtgcctg agcctgagcg gggacgatcg gtctgcgcac
    27061 aactcctttc gcctcgcttc agcaccggtt ggggcattcg cacgctcgca gacgaccaac
    27121 ctcgtttcaa ccccatgtcc tatcacaacg gctcggtctg gcctcacgac acggccattg
    27181 ccgcggctgg cattgcccgc tatggcggcg gcgccgaggt cgccgctttg atccgggacc
    27241 tgttcgaggc cgccaaccac ttcggcatgc gcctgcccga gctgtattgt ggattccggc
    27301 gggcggatgc cgagggacct actccctatc cggtcgcctg cctgccgcag gcgtgggctg
    27361 ccggctcggt cttcatgctg ctgcaggctt gcctcggaat cgatatcgat ggcggccgca
    27421 gaacggtcaa catcacacgc ccgcagttac ctgtcggaat cgagtcgttc tgcatcgcgg
    27481 ggctgccggt cgcacagtcg cgcatcgacc tggagttcca gcgaatgggt cacgaggtcg
    27541 ccgtggttcc ctccggtcat ctcgacagct ccatacgtgt gctcgtgtac ctttgatccg
    27601 gcgctgcaga cgcgcgccta cgcaagcttc tacggaacct gaggcacctt gcagcattga
    27661 gagcacaggc tgaccccgga ggtcgtcgtg gcaagaaagt acggcaagtc cagcggacgc
    27721 aaggtcaaga gggcgatgca cgagatgaag cgcggcaagt tgcgcagtgg tcgctcgggc
    27781 gccaaagtga agagccgcaa gcaggcgatc gcgatcggcc tgtcggaagc tcgccgctcg
    27841 ggcgcgaagg ttccgaaaaa gaagacggga cggaagagcg ctcgcaagaa gagatagccg
    27901 cgcggacgac gcaagcgcgg gacttccccc gatgagcctg ccggagctca ccaagaccgc
    27961 cttgggtgag ctgcgcatgc agatgctggg cgtgcaggtc ctgctcggct tccagttcca
    28021 gagtttgttt cagcagcgct ttgtcgagct cacgcgcgga ggccggatcg tcgatgccgc
    28081 gggtctgctt ctgctcctga tcgccctcgt ggtcctgatc tcgataccgt gccgtcatcg
    28141 gatcgtcgag aaaggggaag ccacgccgca gctgctcaag gtgacacggc aggcagccaa
    28201 tctggccctt gcaccgctgg caggcggact ggcgtgcgat gtgtgggtaa gcatccggat
    28261 cgacttcggc ccgacccaga gcacgctcat ggcggtcgtg gcgggcttgg tggccacgct
    28321 ggcctggttc gctctgccgc gcctgcttcg cagcactcca actgcacagg atccccccat
    28381 gcacccgcag gccacgccac ttcatgccaa gatcgagcag atgctgaccg aagcgcgcgt
    28441 ggtcctccca ggatcgcaag cgctgctcgg ttttcagatg gtggtcatgc tgacacccgc
    28501 gttccgggaa ctgccgcccg cggcgcggga cgttcacgcc gcggctctgc tctgcaacgc
    28561 gctcgcgatc gtgctgctca tctgcccggc cgccgtccat cggatcgcct ttcgcggtga
    28621 ggacaaccca cggtttcacg gcatcggctc gcaactgatc ggcgcggccc tggcgccatt
    28681 ggccgtcagc ctggcctgtg agacctatgt tgggttgctc aagctgttcg gggatgctcg
    28741 caatgcggcg ctcgccggct gcctggtcct gctctgcatg ttggctgtgt ggtatctcct
    28801 cccgctcggc attcgaaaac gggtgcagaa cctgccgttc gcgccggacc gggctcagcg
    28861 ttaggccagc cgatttggcc gtggacgggt cagtccctcg gtccaacgtg gcggaagcgc
    28921 ccgcccggcg ctcgaagatg caggcttcga ccgccacatc accaagcctg ccgatatggc
    28981 ttccctgcag gcggtgctcg cagaggagcg gggtaccgcg gtgtagtgat ttccgggtgg
    29041 gtggtggagc caggcgggat cgaaccgccg acctcttgca tgccatgcaa gcgctctccc
    29101 agctgagcta tggcccctaa gtccctatat tctataggaa acatctgaag ggaagggaat
    29161 gggtcgcggg cgaccgaagc accctcccca acggaggccg cgcagaatac cagaatttcc
    29221 cgggggctgg tcggaccctg agtggacttg cgacttgcgc aaaaatctca cgccgggacg
    29281 catcctgcgc cgacggcgtg agtatcctcg caatgagctt gtgttagcgc ggcgtagttg
    29341 aggtacgcat cgcggtcgca cccgaggcta cgttgtcagg aagcgcaaac acaaacaggc
    29401 tgccgcccag gttggtgaat cgggcgagat ccttgtagcc gcccacggcg cccagcccag
    29461 cggtggcttc cttgaggccg gccgccatac caatgccggc ccaaccgccg atgccggagt
    29521 acacgccgac gtactgcttc ccgttgtact cgtacgtgaa gacgttgccg atgatcccgg
    29581 agggcatctt gaacttccac agctcatgcc cgtccttcgg gctcacggcc ttgagatagc
    29641 cttcgagcgt gccgtagaac accacatctc cggcagtcgt cagcgcgccg gaccacacgg
    29701 agaagcgctc gggtttggac cagacgatct tgcccgcggc cggatcccac gcgatgaagt
    29761 tacccatgcc gccgtggctg tgcggtgtag gaaacatcgt gagggtggca ccgacatagg
    29821 gctgaccggc cgtgtattcg atccggaacg gctcatagtt catgcacacg tggttggtcg
    29881 ggaccatgaa cagtcccgtc ttgcggtcat agctcgaggg ctgctcatcc ttggagccta
    29941 gcgctgccgg gcagatgtcc ttcacattga catccggtcc gctcgtgccg ggagagtact
    30001 tcttgtccac caccggacgc ccgctcttca tgtccaccat cgtggcccag ttgacatccg
    30061 gatcgtactt ctgcgcgacc agcagcgcgc cggtctcccg gttcagcgtg taggcaaagc
    30121 cgttgcggtc gaaatgcacc agcgccggga ctttcttgcc cttgatggtc acatccacga
    30181 gcgggacttc gttcacgcca tcgaagtccc actcatcgtg cggggtcatc tggtagaccc
    30241 acttcgccat gcccgtatcg aggtcgcggg cgaagatgct catggaccac ttgttgtcac
    30301 caggccgctg cgaagggttc caggtgccgg ggttaccggt gccgtagtag aggagattca
    30361 gcttcggatc ataggaatac caaccccagg tcgtgccccc gccgagcttc cattcatcgc
    30421 cctgccagct cttgagcgaa gaatccttgc ccacgggtgc catgcggccg ttggtccagg
    30481 tcatggtctt cttcgggtcc acgagaatgt cctggtccgg cccggtgctc caggctgtcc
    30541 aggctcgctt gccggtcttg aggtcgtagg cttccaggcg accgcgaacg ccgaactcgc
    30601 ctcccgagat cccggtgatc accttgtcct tgaacacgtg cggcgcgttg gtgttggtct
    30661 cgccgatctt cggatcgccg ttcttcacgc tccacaccac tttgcccgtg ttggcatcca
    30721 gcgcgacgag cgtcgtgtcc gcctgttgca ggaaaatctt gccgtcgccg tatgcgaggc
    30781 cgcgattcac agtgtcgcag cacatgaccg gcacgacgtt cacatcctgc cgcggctcgt
    30841 acttccactt gtacgtttga tccttgagat tcaccgcata gacgttattc ggaaacggcg
    30901 tttggacgta catcgtgtcg cccacgacga gcggcgagcc ctcgtggccg cgcaagacgc
    30961 cggtcgacaa cgtccaggcc acttggagct tgcccacgtt ggatgcgttg atctgattca
    31021 gatcgctcca acgctcgttg gcgtagtcgc cgccttgcgc cgcccagttt gcggagttgc
    31081 tcatcttctg ctcgaggtcg gtggcggcgc ttgcggtggc agcggcgcac acggcggcga
    31141 gcgagacata ggtggttcgg agtgcaagcg tgcgtttcat atctgatact cctgagtgag
    31201 gaggtagata cgccgctact cttgcgagtg cctgctaaat tgaaagactg tctttcacga
    31261 gagcggcacg acacacgcct cttctagaag acgaaagccg cgatcgcatt gccaatcgtg
    31321 tccgcgtcga gtgaggtgcg cgtgcagcgg cacctcaatg ggcagacaca accccgaccg
    31381 ctctgcataa ccgcgattgc actcccaccc ttgcggagtg acgtgcgagt gaggcggcca
    31441 tggagaaggc tgggctgcga tgaccgcacc cgcg
//
LOCUS       DASRHN010000361         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1286778, whole genome shotgun sequence.
ACCESSION   DASRHN010000361 DASRHN010000000
VERSION     DASRHN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286778"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFB37_14615"
     CDS             complement(<1..791)
                     /locus_tag="VFB37_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HZO23732.1"
                     /translation="MKIEPTGIDVIPFQEASLDIWDKKYRLSAKDGSPIDKTMDDTYK
                     RVARALADVELPEVREHWYEQFLWALRRGAIPAGRVTSNAGALEHKPATSTINCTVSG
                     TIRDSMDDILKKVHEAGLTLKSGCGIGYEFSTLRPRGAYVSGAGAYTSGPLSFMDIYD
                     KMCFTVSSAGGRRGAQMGTFDVGHPDAMEFIRAKRENGRLRQFNLSLLVTDDFMDAVR
                     ADREWKLAFPLARREYEAEQPDLTDSSRYLWREWPVHENYVVNDEG"
     gene            1291..1734
                     /locus_tag="VFB37_14620"
     CDS             1291..1734
                     /locus_tag="VFB37_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZO23733.1"
                     /translation="MTLIRYEPWSLVNRLHQSLDQIFGDSLSSPEASSSASVTWVPRV
                     DIHEEKDRFVVLADVPGVEPKDIDITAEKGVLTIRGERRSEKKDTENGYERVERAAGT
                     FLRRFTLPESANTESITAKQTNGVLEVTIPKQPQVQPRRISVDVN"
     gene            1883..3253
                     /locus_tag="VFB37_14625"
     CDS             1883..3253
                     /locus_tag="VFB37_14625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZO23734.1"
                     /translation="MKFIAPACAAALLFSATPLIATAAVPPVAVGETPVPSLSPMIKK
                     VSPAIVNIATRGTIHEKGPQNPLLDDPFFRRFFDVPPESGPRERPFQSAGSGVIVDAK
                     SGYIVTNAHVVENATEITVTLQDGRDIKAEVVGSDEPSDVAVLKVKSGNLVQIPLGDS
                     AKVEVGDFVVAIGNPFGLQHTVTSGIISGLSRSGINPDGYEDFIQTDASINPGNSGGA
                     LVNLRGELIGINTAILSRSGGNIGIGFAIPVNMAHSVMDQLIKYGSVKRGLLGVSMYT
                     VTPDIAQSLGLANAQGALVSQVVEGSPAEKAGIHTGDVITSVNGQSVKSNGELRNAIG
                     LMRVGEKVEIGLLREGKPLHVTAVISDNTATTQNSNSSPEAIHRDFEGAALVDAPDSG
                     GALVRSVEPGSAAAQVGLRSNDVIVGANRGRVTNLQQLRDRAKASSVLVLEVRRGNTI
                     VLIPLR"
     gene            complement(3170..4327)
                     /locus_tag="VFB37_14630"
     CDS             complement(3170..4327)
                     /locus_tag="VFB37_14630"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZO23735.1"
                     /translation="MLTESVHALRALLIMVIVAGLQGCGTLYLAQAASGQWHLLRERR
                     PIGSVIADPGTPETLRKRLINVEEARDFAVSELHLPDNRSYRTYADIERPYVVWNVVA
                     APEFSVQPARWCFPIAGCVAYRGYFSERRAREFAAHLASKGYDVTIGGVPAYSTLGKF
                     ADPVLSSMMRYGDSELAAIIFHELAHQLLYVQDDSEFNEAFATTVEDAGLERWLKLRN
                     EPNRLQQFRKEDVEERAFVDLFVQGRAQLARLYASGMGREEMREQKARLLGALADQVR
                     ALQRRQGNEDYEAWLREGLNNAQLASIATYYECVPGFEKLLMQNDGDLLRFYAAARAL
                     AHEPRADRHAKLCTPDSGSSGPEVSSQRNEYDRVAASHLEDQHAGGLGAIP"
     gene            4334..>4771
                     /gene="cca"
                     /locus_tag="VFB37_14635"
     CDS             4334..>4771
                     /gene="cca"
                     /locus_tag="VFB37_14635"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645737.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HZO23736.1"
                     /translation="MEVYLVGGAVRDRLLGLPVHERDWVVVGAQPQELERAGYLSVGK
                     EFPVFLHPQTHEEYALARVERKVAPGYRGFTTQASPDVTLEEDLKRRDLTINAMAETS
                     SGEVIDPYGGQSDLRARLLRHVSDAFVEDPVRILRVARFAARFA"
BASE COUNT          879 a         1551 c         1483 g          858 t
ORIGIN      
        1 ccctcgtcgt tgacgacgta gttctcgtgt accggccatt cccgccacag gtacctggag
       61 ctgtccgtca gatccggctg ctccgcctcg tactcccggc gtgccagcgg gaaagccagc
      121 ttccactcgc gatcggcccg caccgcgtcc atgaaatcgt cggtgaccag cagcgacagg
      181 ttgaattgac gcagccgccc gttctcgcgc ttcgcgcgga tgaactccat cgcatcggga
      241 tgcccgacat cgaacgtgcc catctgggcg ccgcgccgtc cgcccgcgga ggacaccgtg
      301 aagcacatct tgtcgtagat atccatgaac gacagcggac cggaggtata ggcgccggcg
      361 cccgagacgt aggcgccacg cggcctcagg gtcgagaact catagccgat gccgcagccg
      421 ctcttcaggg tcagacccgc ctcgtggacc ttcttgagaa tgtcgtccat cgagtcgcgg
      481 atcgtgccgg acaccgtaca gttgatcgtg gatgtggcgg gcttgtgctc gagcgctccg
      541 gcattcgagg tgacccggcc ggcggggatg gcaccgcggc gcaaggccca caggaattgc
      601 tcgtaccaat gctcgcgcac ctcgggcagc tcgacatccg ccagcgcgcg agccacgcgc
      661 ttgtaggtat cgtccatcgt cttgtcgatg ggcgagccgt ccttcgcgct cagccggtac
      721 ttcttgtccc agatatcgag cgatgcttcc tggaaaggaa tgacatcgat gcctgtgggt
      781 tcgatcttca cgacggtgtt catgaatccg cggcgctccg aatgttgtac gcctgtccga
      841 cggatcacag gggcagcgag cctccggagg aaggcacgca cgctggcgtg ctcacgccct
      901 ctacccggat gaccggaccc cctgacctct ccgatcgctc gatccttggc tcaccttgcg
      961 tgaacacaag atcttgtgtc ggggtcgaaa gactaatgag cactccggtg gccgtcaagg
     1021 cttgcgagca gattctgtca tgtcattttt atttatatta ttcaatgaat tacgagattc
     1081 agcgccgcag tgagcagccg atttccgcag cccggacccg gcttgcgtcg caggtgccgc
     1141 cccaagatat tgtgtgctca ggcaacgcac agcttgtgca catcatgtcc ccacgcgacg
     1201 cttcggctta ccctcgcgga acccttgaaa gcgccagctc cggccacaca tcgcctacaa
     1261 ccaacttcag cgccttcgag gaggaatgac atgaccctga tacgttacga gccctggtcg
     1321 ctcgtcaatc gactgcatca atcgctcgat cagatcttcg gtgactctct gagctcgccg
     1381 gaggcatcca gctccgccag cgtgacctgg gtgccgcgag tggacatcca tgaggagaag
     1441 gaccgcttcg tcgtcctggc ggatgtcccc ggcgtcgagc cgaaggatat cgacatcacg
     1501 gccgagaagg gcgtgctgac gatccgcggt gagcgacgct cggaaaagaa ggacacggag
     1561 aatggctacg agcgcgtcga gcgcgccgcg ggcaccttcc tgcgtcgctt cacgctgccc
     1621 gagagcgcga acaccgagtc catcacggcg aagcagacca atggcgtgct cgaggtgacc
     1681 atcccgaagc agccccaggt gcagccccgc cgcatctcgg tagacgtgaa ctgacaggcc
     1741 ccccgacgtg cggcggcgca acgctgcgcc gccgcagacc gcaggattat gaccactgcc
     1801 cgctgtaggg atcggcgggc cgagaacggc tagaatccag gcgatacgca tcaaacttcc
     1861 ccggcaccgt ccggcgctca ctatgaaatt cattgcaccg gcttgcgcgg ctgcccttct
     1921 gttttcggct accccgttga tcgccacggc ggcggtgccc ccggtagcgg tgggcgagac
     1981 gcccgttccc tccctttctc caatgatcaa gaaggtctcg ccggcgatcg tgaacatcgc
     2041 cacccgcggc accatccatg agaagggccc gcagaatccg ctgctggatg atccgttctt
     2101 ccggcgtttc ttcgacgtcc cgcccgagtc ggggccgcgc gagcgcccgt tccagagcgc
     2161 cggctccggg gtcatcgtcg atgccaagag cgggtacatc gtcaccaacg cgcacgtcgt
     2221 cgagaacgcc accgagataa cggtcacgct gcaggacggg cgtgacatca aggctgaggt
     2281 ggtcggcagc gatgagcctt ccgatgtcgc cgtactcaag gtgaagagcg gcaacctggt
     2341 gcagattcct ctgggtgatt cggccaaggt ggaggtcggc gacttcgtgg tggcgatcgg
     2401 caaccctttc ggcctgcagc acacggtcac ctccgggatc atcagcggtc tatcccgctc
     2461 gggaatcaac cccgacggtt acgaggactt cattcagacc gatgcgtcga tcaatccagg
     2521 gaattcggga ggcgcgctcg tcaatctgcg aggcgagctg atcggtatca acaccgcgat
     2581 cctctcccgc agcgggggca acatcggaat cggcttcgct atcccggtga acatggccca
     2641 cagcgtgatg gaccagctca tcaagtacgg ctcggtgaaa cgcggcctgc tgggcgtcag
     2701 catgtacacg gtcacgcccg acatcgctca gtcactaggt ctggctaacg cgcagggtgc
     2761 gctggtgtcg caggtggtcg agggctcacc cgccgagaag gccggcattc atacaggcga
     2821 tgtcatcacg tcggtgaacg gccagtccgt gaaatccaac ggcgagctgc gcaatgcgat
     2881 cgggctgatg cgggtgggtg agaaggtcga gatcggactg ctgcgggagg gcaaaccgct
     2941 gcacgtcacg gcggtgatct ccgataacac ggccaccacg cagaacagca acagctcgcc
     3001 ggaagccatt caccgcgact tcgaaggcgc agcgctggtc gatgccccgg attcgggggg
     3061 tgctctggtg cgtagcgtcg agcccggcag cgccgcggcg caggtcggtc tgcgcagcaa
     3121 cgatgtcatc gtcggcgcca accgcggccg cgtcactaac ctgcagcagc tacgggatcg
     3181 cgccaaggcc tccagcgtgc tggtcctcga ggtgcgacgc ggcaacacga tcgtactcat
     3241 tcctttgaga tgagacctcc ggtccgctgg atccggaatc gggtgtgcac aacttcgcat
     3301 gcctgtctgc tctgggctca tgcgccaacg cgcgggccgc agcgtagaaa cgcagcaggt
     3361 cgccgtcgtt ctgcatcagc agcttctcga atcccggcac gcactcgtaa tacgtggcaa
     3421 tcgaggcgag ctgcgcgttg ttgaggcctt ccctcagcca cgcctcgtag tcctcgttgc
     3481 cctgccgccg ctgcaaggcg cgcacctgat cggccagagc acccagcaat cgcgccttct
     3541 gctcccgcat ctcttcgcgg cccatcccac tggcgtacag ccgcgcaagc tgcgcccgac
     3601 cctgtacgaa cagatcgaca aacgcgcgct cctcgacatc ctctttccgg aactgctgca
     3661 gccggttcgg ctcattgcgt agcttcaacc atcgctccag ccccgcatcc tccacggtgg
     3721 tcgcaaaggc ctcgttgaat tccgagtcgt cttgcacata cagcagctga tgggcgagct
     3781 cgtggaagat gatggccgca agctcgctgt cgccgtagcg catcatcgag ctgagcaccg
     3841 ggtcggcgaa cttacccagc gtggagtacg cgggtacgcc gccgatcgtg acgtcatatc
     3901 ccttactggc gaggtgcgcg gcaaattcgc gcgcccgccg ttcactgaag tacccgcggt
     3961 acgctacgca accggctatc gggaagcacc agcgggcggg ctgcactgag aactccgggg
     4021 ccgccacgac gttccacacg acgtagggac gttcgatatc cgcgtaggtt cgatagctgc
     4081 gattatccgg gaggtgcagc tcgctcacgg cgaaatcgcg ggcctcctcg acattgatca
     4141 ggcgcttgcg cagcgtctcc ggagtacccg gatccgcaat caccgagccg atggggcgtc
     4201 gttcacgcaa caggtgccac tggccgctgg ctgcctgggc caagtagagc gtgccgcagc
     4261 cctggaggcc ggccacgatg accatgatga gcagggcgcg cagtgcatga acgctctcgg
     4321 ttaacatctg tgaatggaag tctacttggt cggcggcgcg gtgcgtgatc gactcctggg
     4381 gctgccggtg catgagcgtg actgggtagt cgtcggagcg cagccgcagg agctggagcg
     4441 ggccggatac ctgtcggtgg gcaaggaatt tccggtcttc cttcatccgc agacgcacga
     4501 ggaatatgcc ctggcgcgcg ttgagcgcaa ggtcgctccg ggctatcgcg gattcaccac
     4561 acaagcctcg cccgatgtca cgctggagga agatctcaag cgacgcgatc tcacgatcaa
     4621 cgcgatggcg gaaacctcca gcggggaggt catcgatcct tacggcggcc agagcgatct
     4681 gcgcgcgcgc ctgctgagac atgtgtcgga tgcattcgtc gaagatccgg tgaggatcct
     4741 gcgcgtggcg cgcttcgctg cgcgcttcgc g
//
LOCUS       DASRHN010000362         4453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_955610, whole genome shotgun sequence.
ACCESSION   DASRHN010000362 DASRHN010000000
VERSION     DASRHN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955610"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFB37_14640"
     CDS             complement(<1..691)
                     /locus_tag="VFB37_14640"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HZO23737.1"
                     /translation="MTKPPPILANKDYAAVSAFSPENLLREARHQKGLKNGVVPDVRV
                     LDPDGDIVRQTRTVGRAIRDPDWACYHTELYRLTHEGLGLGIVPCAVGASFAVLIAEQ
                     LFASGCQLLVSITSSGELVELRSPPYFILIDKALRDEGTSYHYLPPTEFSSAAPGLIE
                     AMTGAFNGLGVPVERGATWTTDAPFRETPVAICSDEGQGPPGSRDGSCRALRLCRGPG
                     QAGDLPCACDQS"
     gene            complement(688..1416)
                     /locus_tag="VFB37_14645"
     CDS             complement(688..1416)
                     /locus_tag="VFB37_14645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO23738.1"
                     /translation="MADAPSAELGELPAAYQAWRASDLGRITDRIEERLILTLVGAVQ
                     GKHVLDVGCGDGALSVVLAKAGGDVAGLDANPRMLAAARDRAGRVDAAVTFIEGYARQ
                     LPFADDTFDVVVAITVLCFVADAERAVNEMARVLRPGGRLVIGELGRHSLWAVKRRLS
                     GWIGSRTWRAALFRSARALKQLARASGLDVNEIRGAIYYPPCHVCARLLAPLDARLAA
                     VTTFGAAFIALAARKPHHRSQGRS"
     gene            complement(1418..2548)
                     /locus_tag="VFB37_14650"
     CDS             complement(1418..2548)
                     /locus_tag="VFB37_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZO23739.1"
                     /translation="MRPIYLDYNASTPIAPEVVAAMRPFLDGAYGNPSSGHWAGNPAR
                     EAVERARGHIAHMLGCASNEVVFTSGGSESNNHALKGVTFALRDKGDHIVTSTVEHPA
                     TLVPCEHLARLGYKITHVPVDGLGRVAPDDVRRALTPRTILVSIMHANNEVGTIQPIE
                     DIGRITRERGIVLHTDAAQAVGKILTKVDELGVDLLSIAGHKLHAPKGVGALYIRNGT
                     PIEPLIHGGGHEGGRRAGTESALMTAALGAAAELAANLPPMRRIRDLRDDFWRQLKAA
                     LGDRVVLNGHPQHRLPNTLNVSFVGRIGADVLASLDGVAASTGSACHSGRVELSPVLR
                     AMGTSPQIGMGAVRFSLGRETTTEEIDTVVAMINQTLGAPGR"
     gene            complement(2551..2904)
                     /locus_tag="VFB37_14655"
     CDS             complement(2551..2904)
                     /locus_tag="VFB37_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZO23740.1"
                     /translation="MKVLFIINDPPYGTERAYNALRLAPALVKKDPETQITVFLMADA
                     VLAAKAGQKTPEGFYNVERMLKRVLAAKGKVLLCGTCMDARGLTDTELVEGAHRSTMD
                     ELAGDTLAADKVLVF"
     gene            complement(2915..3916)
                     /locus_tag="VFB37_14660"
     CDS             complement(2915..3916)
                     /locus_tag="VFB37_14660"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZO23741.1"
                     /translation="MKLEAALPRLLLVAGIVAAAAVLAHNRDRLDIGAIEQLTRHLGV
                     WAPIGHVVLFAAGTVLFAPGAIFGLAGGVLFGPFVGTLLNLAGATLGATAAFLVARYI
                     AADWVRARTGARIERLISGVEAEGWRFVALMRLVPLVPFNLLNYALGLTRISLPQYVL
                     ASLIAMAPGTLAYTWLGYAGRQALGGETAAIRYGLLALGLLAAIAFVPRLVSRLRSNT
                     AHRWIEVSELARLLADKGGVAVVDVRSPDDFRGPLGHIAGARNLPLTDLQSRLAEIAA
                     LKNEPVVLVCRTHRMSATAAAQLGMVGFRDIRVVRGGMVQWNEQGLPIADRLEQRET"
     gene            complement(3913..>4453)
                     /locus_tag="VFB37_14665"
     CDS             complement(3913..>4453)
                     /locus_tag="VFB37_14665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012517611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZO23742.1"
                     /translation="AIYLQHVLVHAVPALWRLHRMHHADLEFDVTTGARFHPIEILLS
                     MVLKLMVIAALGPPAAAVLLFEVLLNATAMFNHSNVRVPDGLDRVLRLIVVTPDMHRI
                     HHSVVPKETNSNFGFNLPWWDRLFGTYRPEPAAGQLGMTIGIEQFRDATEQRLDHMLT
                     QPFREGDRSYAFGQREPAE"
BASE COUNT          800 a         1447 c         1493 g          713 t
ORIGIN      
        1 ctgattggtc acatgcgcaa ggcagatcac cggcctgccc cggacctcgg caaaggcgta
       61 gagcgcggca gcttccatct cgactgccag gaggcccttg gccttcatcg ctgcaaatgg
      121 cgaccggagt ctcacggaac ggtgcgtcgg tcgtccaagt cgcacctcgc tcgaccggga
      181 cgcccaagcc gttgaatgcc cccgtcatcg cctcaatgag gcccggcgcg gccgagctga
      241 actctgtcgg gggcaggtag tggtagctcg tgccttcgtc gcgtagcgcc ttgtcgatca
      301 gaatgaagta tggcggcgac cgcaactcaa cgagttcacc ggacgacgtg atgctgacca
      361 gcagttggca gcccgatgcg aacaactgct cggcgatcaa gacagcgaac gacgcgccga
      421 cagcgcaggg cacgatgccg agcccaaggc cctcgtgggt caagcggtaa agctctgtgt
      481 gatagcaggc ccaatcggga tcgcggatgg cgcgaccgac ggttctggtc tgccggacga
      541 tgtcaccatc cggatcgagc acgcgcacgt ccggaaccac accgttcttg aggcctttct
      601 gatggcgcgc ctcgcgcagc aggttctcgg ggctaaaggc cgagaccgcg gcgtagtcct
      661 tgttggcgag gatgggagga ggcttcgtca tgagcgtccc tgcgaacgat ggtgcggctt
      721 tcgcgcggca agtgcgataa aggccgcacc gaaggttgtc accgccgcga gacgggcatc
      781 cagcggcgcc agcaggcgtg cacagacatg gcacggtggg tagtagattg cgccgcgaat
      841 ctcgttgacg tcgaggccgg acgcccttgc gagctgcttc agggcgcgag cagatctgaa
      901 cagggcggcg cgccatgttc tcgatccaat ccagccggag aggcggcgct tcactgccca
      961 tagcgagtgg cgtccaagct cgccgatcac caggcgaccg ccgggccgca gcacgcgcgc
     1021 catctcgttc accgcacgct cggcatcggc cacgaaacac agaacggtga ttgccaccac
     1081 gacatcgaac gtgtcatccg cgaacgggag ctgtcgtgcg tatccttcga tgaatgtgac
     1141 cgcagcatcc accctgccgg cgcgatcacg cgcggcggcc agcatacgcg gattggcatc
     1201 gaggccagcc acgtccccgc cggctttggc aagcacgacg gacagcgccc catcaccgca
     1261 gccaacgtcc agcacatgct tgccctggac agcgccaacc aatgtaagga ttagccgctc
     1321 ctcgatccgg tcggtgatgc ggccgagatc gcttgcccgc cacgcttggt aggcggcagg
     1381 aagctcgcca agctctgccg atggagcgtc cgccatgtca gcggcccggt gcaccgagcg
     1441 tctgattgat catggcaacg acggtgtcga tctcctccgt cgttgtttcg cggccgaggc
     1501 tgaagcgcac ggcgcccatg ccgatctgcg gcgaagtccc catcgcccgc agcactggtg
     1561 acagctcgac gcgcccgctg tggcaggccg agcccgtcga cgccgccacg ccgtcgaggc
     1621 ttgccagcac gtcggccccg atcctgccga cgaaggagac gttgagcgtg ttgggcaagc
     1681 gatgttgggg gtggccgttc aggacgacgc gatcaccgag agccgccttg agctgccgcc
     1741 agaagtcatc gcgcagatcg cggatgcgtc tcatgggcgg gagattggcg gcaagctcgg
     1801 ccgccgcacc gagcgccgcc gtcatcagag cgctctcggt accggcgcgt cggccgccct
     1861 cgtgaccgcc gccgtggatc agcggctcga tcggcgtgcc gttgcgaatg taaagggcgc
     1921 cgacgccctt gggagcgtgg agcttgtggc cggcgatcga gagcaggtcg acgccgagct
     1981 catcgacctt ggtcaggatc ttgccgactg cctgcgccgc gtcggtgtgc aggacaatgc
     2041 cacgctcccg cgtgatccga ccgatatcct cgatcggctg gatcgtgccc acctcgttgt
     2101 tcgcatgcat gatgctgacg aggatggtcc gcggcgtcag ggcgcgccgg acatcgtcag
     2161 gagccacacg gccgagccca tccaccggca cgtgcgtgat cttgtagccg agccttgcca
     2221 ggtgctcgca gggcaccagc gtagccgggt gctccacggt cgaggtgacg atatgatcgc
     2281 ctttgtcccg cagcgcaaag gtcacgccct tcagggcatg attgttggac tcgctgccac
     2341 cgctggtgaa gacgacttcg ttgctggcgc atccgagcat atgcgcgatg tggcctcttg
     2401 ctcgctccac ggcctcgcgc gcagggttgc cggcccaatg cccgctcgat gggttgccat
     2461 aggcgccatc gaggaaggga cgcatggccg ccacgacctc gggcgcgatc ggcgtgctgg
     2521 cattgtagtc gagatagatg gggcgcatgc tcagaacacc agcaccttgt cggcggcgag
     2581 cgtgtcgccg gcgagctcat ccatggtcga gcgatgcgcg ccctccacca gctccgtatc
     2641 ggtcaggccg cgggcatcca tgcaggtgcc gcagagcagg accttgccct tggcagccag
     2701 cacgcgcttc aacatgcgct cgacgttata gaagccttcc ggcgtcttct gcccggcctt
     2761 ggccgcaagc acggcatcgg ccatcagaaa cacggtgatc tgcgtctcgg ggtccttctt
     2821 gacgagtgct ggcgccagcc gcagcgcatt gtaggcgcgc tccgtgccat agggcgggtc
     2881 gttgatgatg aacagcactt tcacgcttgc ctcctcatgt ttcccgttgc tcgagccgat
     2941 cagcgatcgg caggccttgt tcgttccact gcaccatgcc gccgcgcacc acgcggatgt
     3001 cacggaagcc gaccatccca agctgagcgg ctgcggtggc cgacatcctg tgcgtgcgac
     3061 agacgaggac taccggctcg ttcttcaagg ccgcgatctc ggccaggcgc gactgaagat
     3121 cggtcagtgg cagattgcgg gcgccggcaa tgtggccgag cggccctcta aagtcgtctg
     3181 gcgagcgtac gtcgacgacg gcgacgccgc ccttgtcggc gagcagccgc gccagctcgg
     3241 aaacctcgat ccagcgatgc gctgtgttgc tgcggagccg gctgacgagc cgtggaacga
     3301 aggcgatggc cgccagcagc ccgagcgcaa gcaggccata acggatggcg gcagtctcgc
     3361 cgccgagcgc ttgccgtccg gcgtagccga gccaggtgta ggctagcgtg ccgggagcca
     3421 tggcgatcag cgaggcaagt acgtactgcg ggagggagat tcgggtcaga ccgagtgcgt
     3481 agttgagcag gttgaacggc accagcggga ccaggcgcat gagagcgacg aagcgccagc
     3541 cctcggcctc gacaccggag atgaggcgct cgatgcgggc gccggtcctc gcccgcaccc
     3601 aatcggctgc gatgtagcgt gctacgagga aggcggcggt cgctccgagc gtcgcgccag
     3661 cgaggttgag cagcgtcccc acgaacggcc cgaacaacac gccgccggcg agaccgaaga
     3721 tcgcccccgg cgcaaagaga accgtccctg ccgcgaacag gacgacatgg ccaatcggcg
     3781 cccaaacgcc gagatggcgc gtcagctgct cgatggcccc gatatcgagg cggtcgcggt
     3841 tgtgggcgag aacggccgcg gcggcgacaa tgcccgccac gagcaacaga cgcggaaggg
     3901 cggcctcgag cttcattcag cgggctcccg ctggccgaag gcatagctgc gatctccctc
     3961 gcggaatggc tgcgtcagca tatggtcgag gcgctgctcg gtggcgtcac gaaactgctc
     4021 gatgccaatc gtcatgccca gctgtccggc ggcgggctcg gggcgatagg tgccgaacag
     4081 tcgatcccac cacggcagat tgaagccgaa attggagttg gtctctttcg gaacgacgga
     4141 gtgatggatg cggtgcatgt cgggcgtgac cacaatcaat cgcagcacac ggtcgagccc
     4201 gtcggggacg cgcacgttcg agtgattgaa catggccgtt gcattgagca gcacctcgaa
     4261 taggagcacg gccgcggcag gcggccccaa tgccgcgatc accatcagct tcagcaccat
     4321 cgacagcagg atctcgatgg ggtggaagcg cgcccccgtc gtcacatcga actcgagatc
     4381 ggcgtgatgc atgcggtgca gacgccacag cgccggcacg gcgtggacca agacgtgctg
     4441 caggtagatc gcg
//
LOCUS       DASRHN010000363        18701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1948276, whole genome shotgun sequence.
ACCESSION   DASRHN010000363 DASRHN010000000
VERSION     DASRHN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948276"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFB37_14670"
     CDS             <1..975
                     /locus_tag="VFB37_14670"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZO23743.1"
                     /translation="YSDRVYHCTAIGAGRALDGIRMMAIARGLSLEQMAQSPGVITVI
                     SVNSPRRFDAAMADGLMTMAEHAQAVVITPFTLMGAMAPVSLAAALAQQNAEALFGAV
                     LAQLVRPGTPIVYGAFTSNVDMRSGAPAFGTPENAKANAASGQLARRYRLPYRTSNAS
                     ASNAADAQGAYETQMSLWGAILGHGNLVYHAAGWQEGGLTASFEKLIMDVEMLQLMME
                     FLQPITVNEAELGFEAIRGVRTGGHFFGEPHTMERYEHAFYQPLLSNWQNYESWQLAG
                     GQDATQRATGIWKRALQEYTEPALDPAIREELDAYIARRRDEIGSGEP"
     gene            complement(978..2990)
                     /locus_tag="VFB37_14675"
     CDS             complement(978..2990)
                     /locus_tag="VFB37_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZO23744.1"
                     /translation="MRLTLLACLPLCSACHTLAAERPAPLGPARDVTTVVPAPSSDEA
                     DRVRPEFIAAMHRVRQHLPEPADSNPLKAYVIYDYLVAARLERDLELKPGEDLDVQVD
                     AFLKEHSGQPVTRNLRTEWLESLATRGRWDWFLPRASDLTSPVLLCDRLAAELASGAT
                     GTLAAEALARWSLPQKQPAECDPVFNWLSAQGLLTAPLAQTRARAALAAENSKLARES
                     MTLLTSEQAAPLLEWAQLLESPKSALTALAAEPSRPVEASALEAGFTRLSRLDSATAS
                     TLLPGLLRRPDLAPELRTRLRRLAALGAAYDHDPATVETFRSLPPEAADTEIQEWRVR
                     AALWAGNYDVALEWMDQMTPALSQQPRWRYWRARALESTVGAAAAAPLYGQIAGLRDF
                     YGYLAADRLHMGYALNGHPSAEDAAIQDALAARPGLIRAHELFECRLTEEALVEWAAT
                     LGSAPPAVRLQAAHLAARWGWYAQSIATLAQVGELDDVQLRYPRPYGAAVEAASKLTQ
                     LPPDWILAVMRQESLFRPDALSRAGARGLMQMQPATARAVARRWHVRSPPKQPEGAID
                     PEPDVALGAAYLRELLDRYGNQLGPSLAAYNAGPVSVSRWLPARAVDADVWIENIPYT
                     ETRNYVERIFEHIVAFAWVRNAQPPRLAALLPPVEPNTFYAQAGQP"
     gene            complement(3009..3536)
                     /locus_tag="VFB37_14680"
     CDS             complement(3009..3536)
                     /locus_tag="VFB37_14680"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HZO23745.1"
                     /translation="MATSPSHLRLVHGDITTLDLDAIVNAANSSLLGGGGVDGAIHRA
                     GGPQLLQACRQLGGCDVGDAKLTPGFRLKARHVIHTVGPVWRGGGRGEAQLLAACYRR
                     CLQVAAEHRLQSIAFPSISTGAYGFPRRLAAPIAIAAAQAPDVSGSSVHEVIYCCFSH
                     EDFEIYRELLRCGAG"
     gene            complement(3561..4310)
                     /locus_tag="VFB37_14685"
     CDS             complement(3561..4310)
                     /locus_tag="VFB37_14685"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO23746.1"
                     /translation="MADQHPADLAGSRVAACIGEPARARMLYCLLDGHARTSTELAIV
                     AGVSASTASVHLARLRRERLVTAMAQGKHRYYRLEGPKVAAALEALMVIAGEPRARFV
                     PRTPNRLRFARTCYDHLAGEIAVLLHDRLRELRWISAAPVADEYELSAEGARALAMLG
                     VNVDAARAQRRRFAYACVDWSERRPHMAGALGAALLQLALKRRWVVQELDSRALRLTR
                     TGSGELLTRFGLSMPDHRSNGASARAGESRV"
     regulatory      4761..4849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4969..6813
                     /locus_tag="VFB37_14690"
     CDS             4969..6813
                     /locus_tag="VFB37_14690"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HZO23747.1"
                     /translation="MQAFAFLAGAHLRNALLCAVVAASLAACGGDDAGSGAQATGLQQ
                     TSQGTNHFSPTIEGVPAVSVDVGSRYVFLPSATDANGAPLRYSVSNKPLWASFDTGTG
                     MLSGTPSQADIGDTVGISVIVTNGQGQSSIGPFNLRVRPHPGRPLPSPQPTEPVTDSP
                     PLISGVPMSTVIVGQVYSFTPRASDPDGDTLSFTIANRPDWATFDSSTGRLAGIPTVG
                     SEGTFQQIVISVSDGQQVSALPPFSIQVMPPAAHTPSISGMPPTSVVAGQTYTFSPSA
                     TDPDGGTLTFEIANEPSWATFNSRTGQLSGTPTVADVGTRAGIVISVTDGQASAELPA
                     FSIQVLASANHPPSISGSPPTSVLAGTEYSFQPSASDADGDPLTFSVKNRPAWATFDA
                     ATGRLSGVPSGTQTGKDANIVITVSDGETSVSLPAFTVTVTGSQLAAPSISGAPTASV
                     NVGSAYGFTPSATAPSGTSLTFSIQNKPSWASFNTGTGALTGTPAAGDVGTYANIIIS
                     VSDGQASASLSAFTVTVNQISSSSATLEWTPPTENTDGTSLTNLAGYKVYYGTSAGSL
                     SHVVQLANPGLTGYTVSSLSSGTWYFAVTAYTSGGVESSLSPVVSASF"
     gene            complement(6829..7815)
                     /locus_tag="VFB37_14695"
     CDS             complement(6829..7815)
                     /locus_tag="VFB37_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZO23748.1"
                     /translation="MRFAIRSAFILGLALLTYLIVRQGAASIITPLLGAGAVLLWIVP
                     LHTLPIVLDVLGWRLLLPPQTRLSTLLWIAAVREAFNRLLPVANIGGEIVGVRLLAQS
                     GTPAPVGAASIVVEVVLTVAAQYMFAVLGVLCLVTAAGPTRLGSELVLALLVSLPLIA
                     ILITVVRFGAVFARLARLGEWMLGRRFRTVAGDNPAALEAAIARLYAHPWRLGATALW
                     QLSGLIAGTVETWLVLRWFGRPVGVPAALALESLSQAARSFLFLVPAGLGVQEASLVG
                     VGQLFGIGNDVAIALSLAKRMREILFGSPALLRWYWMEGRTHWHSGRARRQA"
     gene            complement(7812..8702)
                     /gene="hpnK"
                     /locus_tag="VFB37_14700"
     CDS             complement(7812..8702)
                     /gene="hpnK"
                     /locus_tag="VFB37_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HZO23749.1"
                     /translation="MEKFLIVTADDFGLHEAINAAVDEASVSGVLTTASLMVGAPACA
                     DAIARARALPGLRIGLHLVLADGWSVLPHRLIPALVNRQGRFPNRMFVEGLRYFALPA
                     VRKQLEAEIRAQFEAFASSGLALDHVNAHKHFHLHPTLLEIILRVGTEFGLAAMRVPY
                     EPPALAASGNTLWRGAAALLLAPWLALMRRRLRARDIACNDQVLGLAASGSLGEATLL
                     HALAKLPRGTTEIYLHPALSASGPISSGMRKYRHADELAALLSPRVRAAMEATGASRG
                     GYADLLRMRSQPEPGGSAAA"
     gene            complement(8751..10172)
                     /gene="hpnJ"
                     /locus_tag="VFB37_14705"
     CDS             complement(8751..10172)
                     /gene="hpnJ"
                     /locus_tag="VFB37_14705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HZO23750.1"
                     /translation="MKTLFLHPPSFDGFDGGAGSRYQAKREIRSFWYPTWLAQPAALV
                     PGSRLLDAPADGLNLEQILPLARDYDLIVMHTSSPSFPTDAHVAERLKAENPHLLIGM
                     IGAKVAVDPIGSLSASAAIDFVAREEFDYTCAEVAEGRDLQSIAGLSFKAPDGSIRHT
                     DARPPAADMDALPWVSPVYKRDLTISNYFIGYLQHPYVSFYTGRGCRSKCTFCLWPQT
                     VGGHKYRVRSPASVIEEVRWIQQNLPEVKEIFFDDDTFTDFRPRAQEIARGLGKLGVT
                     WSCNAKANVPYETLQVMKDNGLRLLLVGYESGDDQILRNIRKGLRTDIARRFTADCRK
                     LGITVHGTFILGLPGETAETIRETIRFAVEINPHTIQVSLAAPYPGTELYRQAVQQGW
                     LEEKADLNLVNSQGIQLSPISYPHLSAQEIYHGVETFYRNFYFRPRKIAELTAEMLTS
                     WDLTRRRLREGVEFFRFLRAREA"
     gene            complement(10202..11338)
                     /gene="hpnI"
                     /locus_tag="VFB37_14710"
     CDS             complement(10202..11338)
                     /gene="hpnI"
                     /locus_tag="VFB37_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZO23751.1"
                     /translation="MTWPMAAGLFLAVLALGYSLMAWLAICLLRPGPRRPETPAPEQP
                     PVTVLKPLCGAEADLLEYLRSFCRQDYPKFQIVFGVRDTADPAIEVVRNLQAEFPQLD
                     LVIATDAPQRGSSRKVSNLINMMPLARYEFLVIADSDVRVAPDYLARVVHPLLDARVG
                     IVTCPYVGRPRPGLWSLLASVFVNDWFMPSARIAALFGSRAFAFGATIAIRRQALTRV
                     GGFAAIADQLADDYRLGELTRRVGLRTVLSDVVVETCIAERSLGELVRHELRWLRTIR
                     AVRPLGYTFAFISFGVPLAVAGAILAKGSDLSLLMLALTILARFMLHAVAWKPRLRTA
                     SPWVLPLSDFLAFGLWCWGFVTSSVHWRDIRYRVARDGSLHPIV"
     gene            11884..15210
                     /locus_tag="VFB37_14715"
     CDS             11884..15210
                     /locus_tag="VFB37_14715"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZO23752.1"
                     /translation="MLLRNPSLGQDRIAFLYAADVWTVPREGGEAQRLTSGAHVVEGP
                     YYSPDQQLIAYSTSQHGQIDVYVIAADGGVPRRLTWEPTGNHAVGWTPDSKEILFTSQ
                     HASYSDFPRLFRVRADGVGSPQLVPLPSVDWGSLSSDGNTLAYVPVMRWQNAWKHYRG
                     GQTTPIWLVNLKTLDLEKIPRENSNDSNPVWSGKTVYFLSDRNGPVSLFSYDTDSKQV
                     QPVGENHGFDLKRLSAGPGALVYEQFGSLHLLELGNHQDRVVPVTIRGTLPELAPHLA
                     KVPAKGVDNTAISPTGARIVVEARGDIFTVPAEKGNTRNLTNTPGAAERDPAWSPDGK
                     SIAYFSDASGEYQLYIRDQEGLRPPKVIDLGPDPSFFYAPRWSPDSKHIAYSDKHLRL
                     WYIDVSGGRPVKIDTALRGGFGNNTELSWSPDSQWVAYSRDLESQMRAIFLYSLATHS
                     SHQVTDGMSNADHPVFDPSGKYLYFTASTNNGPSDAGIDLSSLDRATNSSVYVVVLSK
                     TGASPVPPESDDEKGKDDKGKDEKGKDEKGKKGDKDDEDSSKDEHAEADKSGSDETDS
                     KKSSGKKKEEKAKPTVVDLAGIGNRILALPIPARNYQGISVGKTGVLFLAEGAPVGRP
                     SAPDEIPIHSLWKFTTEKRKTEEVLSELSAYAVSFNGEKLFYARHDSWFITPVSDLKP
                     GSPEGTPGKPVDANGMWAMRDPRASWKQMFHETWRIERDFLYDPHYHGLDLQKIEARY
                     QPYVAGLASRAEFTYLSEEMLGEIQLGHVFVRGPPAPPDAPKPGLLGADYTVEHNRYR
                     FAKIYNGQNWTPSLTAPLTQPGINIVEGDYLLAVNGRELYATDNLDSFFDGTAGKQTV
                     LRVGKKPDGSDARDVTVVPVDSEHGLRNLDWIESNRHKVDQLSGGKVAYIWMPNTAGA
                     GYTNFNRYFYAQLDKQAVVLDERYNEGGFIADYIVNVLGQKRLSGAIGREGEPVHDPE
                     GAIFGPKAMIINQSAGSGGDAMPWYFRKAKLGTLVGTRTWGGLVGIGGYPNLIDGGAV
                     TAPRYAIYGLNGEWEVEGHGIPPDVEVEVLPKEVAAGHDAQLERAVAIVMEQLKEHPV
                     VEPRIPAYPNYHQQDALGAH"
     gene            complement(15211..15774)
                     /locus_tag="VFB37_14720"
     CDS             complement(15211..15774)
                     /locus_tag="VFB37_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23753.1"
                     /translation="MRVVGFRVRSGVTLCVLVEGAKESWQVLASCEVPLSPQGGKFAR
                     FPFHPTVELEGRAAIVESRKAVAAVRMTARREMRAALADLAPIEAAGVVGGSLTDPGR
                     IHNPHVQAHAREAQLYREVVTSELKQAGIAFEVLSDRDILARLADLHRTSVAALNRSL
                     TRAGRGTFRPWAAHQKLAAAGALWGLH"
     gene            complement(15830..16795)
                     /locus_tag="VFB37_14725"
     CDS             complement(15830..16795)
                     /locus_tag="VFB37_14725"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZO23754.1"
                     /translation="MMDALSQTLRVVQLVGAIFINARFTAPWCYQSPRADFAAPLLEP
                     GAERVVIFHLVTEGECYVELGREPPLQLKPGDVVVFPDGHAHRMSSQPGLKPATGSRL
                     DAVLARRPRQLAYGGGGAVTRLVCGYLACDARLARMLLAGLPPIVKVNVRGSDAGTWL
                     EASVRYALAEARSPRAGGAGVLAKLAEVLFIEVLRLYMSEQGKGGTGWLAGVGDRIVG
                     AALTLLHKDPAHAWTLEELARKAGASRSVLAERFQHLVGSAPMQYLAQWRMLLAANLL
                     RRSSAPLATIAQEVGYQTDTAFSRAFRREFGSPPAAWRRKHFGHQ"
     gene            complement(16853..17428)
                     /locus_tag="VFB37_14730"
     CDS             complement(16853..17428)
                     /locus_tag="VFB37_14730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZO23755.1"
                     /translation="MLSDDNLEFVYTGARMTTQNVATALLRVESVLQRRPGAGLHDDA
                     PATVRWERGLRVVAGHLNGTQIPTDMPRELGGTGDRVTPGWLMRAGLAACTATSIALR
                     AAARGIELQRLEIVASSRSDTRGLLGMINERGEQVPAGPRDLELTVRIAAQGATAEQL
                     RSLVEDGYRNSPIACALEAAMPVGLRIDIST"
     gene            17620..18480
                     /locus_tag="VFB37_14735"
     CDS             17620..18480
                     /locus_tag="VFB37_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO23756.1"
                     /translation="MDARLQRRIQRYGWDLAASDYEPLWKAQLAEAQKAVLRAASPAR
                     GERVLDLACGTGLVALQAARAVGPHGRVLGIDLSARMIAAARAHALEQAVPNAEFSRM
                     DAEKLDLPARTFDVALCALGFMYLPDPERALLEMRRVLRPGGRIALAVWGERERCGWS
                     GVFPIAAAEVASDVCPLFFQLGQGSALAELCGRLQFDTIHHSRIQATLSYADAATACD
                     AALVGGPVALAWSRFSEEVRDRVRARYLQSIEPWKCGAGYRVPAEFVVVSGVVPQPGG
                     LRIAAGGFSV"
BASE COUNT         3396 a         6140 c         6111 g         3054 t
ORIGIN      
        1 tactcggatc gggtctacca ctgcaccgcc atcggcgccg gcagggcact ggatggtatc
       61 cgcatgatgg cgatcgcccg gggcctgtcg ctggagcaga tggcgcagag cccgggcgtc
      121 atcacggtca tctcggtgaa cagcccgcgc cgcttcgatg cagcgatggc ggatggcctc
      181 atgacgatgg ccgagcacgc tcaggccgtc gtgatcaccc ccttcaccct catgggggcc
      241 atggcgcccg tatcgctggc cgctgcgctg gcccagcaga atgcagaggc gctgttcgga
      301 gcggttctcg cgcagctcgt gcggcccggg acgcccatcg tgtatggcgc gttcacctcc
      361 aatgtggaca tgcgctcagg cgcgcccgct ttcggcaccc ccgagaatgc caaagcgaac
      421 gctgcgagcg ggcagctcgc caggcgctat cggctgcctt accggacctc gaatgcgagc
      481 gcctccaatg cggctgacgc gcagggtgcc tacgagaccc agatgtcctt gtggggcgcc
      541 attctcggtc acgggaatct ggtctatcac gcggcaggct ggcaggaagg ggggctcacg
      601 gcctccttcg agaagctgat catggatgtc gagatgctcc agctcatgat ggaattcctc
      661 cagcccatca cggtcaatga ggccgagctc ggtttcgagg cgatccgggg agtgcgcacc
      721 ggcggccatt tcttcggcga gccgcacacg atggagcgct acgagcacgc cttctaccag
      781 ccgctgctct ccaactggca gaactacgag agctggcagc tcgccggcgg gcaggatgcc
      841 acccagcgtg ccaccggaat atggaagcgc gccctgcagg aatacaccga gccggcgctg
      901 gatcccgcca ttcgcgaaga gctcgatgcc tacatcgccc gacgccgcga cgagatcggc
      961 agcggcgagc cttgatctca gggctgtccg gcctgagcgt agaaggtatt gggctcgacg
     1021 ggaggcaaca aggccgcgag ccgcggcggc tgcgcgttgc gcacccaggc gaaggcgacg
     1081 atgtgctcga agatccgctc aacgtagttg cgcgtctcgg tgtacggaat gttctcgatc
     1141 cacacgtcgg catccactgc gcgtgccggc agccagcgtg agaccgatac cgggccggcg
     1201 ttgtaggccg cgaggctcgg accgagctga ttgccgtagc gatcgagcag ctcgcgcaag
     1261 taagcggcgc ccagagccac atccggctcc gggtcgatag ccccttccgg ttgtttcggc
     1321 ggggacctca cgtgccagcg ccgggcgacg gcgcgggcag tggcaggttg catctgcatc
     1381 aggccgcgtg cgcccgcgcg cgagagcgca tcgggccgaa agaggctttc ctggcgcatc
     1441 acggccagga tccaatccgg cggaagctgc gtgagtttgc tggcggcttc aacggctgca
     1501 ccgtaggggc gcggatagcg cagctgcacg tcatccagct cgccaacctg ggccagcgtc
     1561 gcgatggact gtgcatacca accccagcgc gcggccagat gggcggcctg cagccggacg
     1621 gcgggcggtg cgctgcccag ggtggcggcc cattccacca gcgcctcctc ggtcagcctg
     1681 cactcgaaca gctcatgggc acggatcagg cccggccggg cggcaagagc atcctgaatg
     1741 gcggcatcct ctgcggaggg atggccgttc agagcgtagc ccatgtgcag ccgatcggct
     1801 gccagataac catagaagtc ccgcaacccg gcgatttgtc cgtagagggg cgcggcggcc
     1861 gcggcaccga ccgtggactc cagggcacgg gctcgccagt accgccagcg cggctgctgc
     1921 gaaagagcag gcgtcatctg gtccatccac tcgagcgcga cgtcgtaatt gcccgcccag
     1981 agcgccgccc gcacgcgcca ttcctggatc tcagtgtcgg cagcttccgg agggaggctg
     2041 cggaaggtct ccacggttgc ggggtcgtgg tcgtaggccg caccgagggc agccagtcgt
     2101 cggagccgcg tgcgaagctc cggtgccaga tcgggccgcc tcagaaggcc cggcagcagc
     2161 gtggacgccg tggcggaatc caggcgcgat agtcgcgtga agcctgcttc gagcgccgag
     2221 gcctcgacgg gccgggacgg ctctgcagcc agagcggtga gtgcggattt ggggctttcg
     2281 agcagctgtg cccattccag caagggcgct gcctgctcgc tggtaagcaa ggtcatggac
     2341 tcgcgcgcga gcttcgaatt ctcggcagcg agggccgcac gggcccgggt ctgcgccaac
     2401 ggtgctgtca gcaggccctg ggcgctgagc cagttgaaca ccggatcgca ttcggcgggt
     2461 tgtttctggg gcaggctcca gcgcgccagt gcttcggccg ccagggtgcc cgtggcaccg
     2521 ctcgcaagct cggctgccag tcgatcacac agcagaacgg ggctcgtgag gtccgaggcg
     2581 cgcggtagaa accagtccca gcgcccgcgc gtcgccaggc tctccagcca ttcggtgcgc
     2641 agattccgag tgaccggctg cccgctgtgc tccttcagga aagcgtccac ctgcacatcg
     2701 agatcctcac ccggcttgag ctcgaggtcg cgttccaggc gtgcggccac caggtagtcg
     2761 tagatcacat acgccttgag cggattcgag tcggccggct cgggcagatg ctgacgcacg
     2821 cggtgcattg ccgcgatgaa ttccggccgg acccggtcgg cttcgtcact cgaaggcgcg
     2881 ggaacgaccg tcgtcacatc gcgagccggc ccgaggggtg cgggtcgctc ggcggcgagc
     2941 gtatgacaag ccgagcaaag cggcaggcag gcgagcaagg tcagtcgcat acgaggccgc
     3001 atgaatcttc aacccgcgcc gcaacgcagc agctcgcgat agatctcgaa gtcctcatgc
     3061 gagaagcagc agtaaatgac ctcgtgcacg ctgctgccgc tcacatcggg cgcctgtgcc
     3121 gccgcgatcg cgatgggggc tgcaagtctg cgcgggaaac cgtaggcgcc agtgctgatt
     3181 gatggaaaag cgatgctctg cagccgatgc tcggctgcaa cctgcaggca gcgccgataa
     3241 caggcggcca gcaactgcgc ttcgccgcgt ccgccgccac gccataccgg tcccaccgtg
     3301 tggatgacat gtcgagcctt cagccggaat cccggcgtca gcttcgcatc tccgacatca
     3361 cagccgccca gctgccggca ggcctgcagc agttgcgggc cgccggctcg atgaatcgcg
     3421 ccgtccacgc cgcctccgcc cagcagagag gagttcgcgg cattgacgat ggcatcgagg
     3481 tcgagcgtgg tgatgtctcc atgaacgagg cgcagatgcg agggcgaagt ggccatgcca
     3541 gtgggctgcc gcggttgaga tcagacccgc gactcgcccg cgcgggcact cgcgccattc
     3601 gagcgatgat ccggcatgct cagcccgaac cgtgtaagca attccccgga acccgtgcga
     3661 gtcaggcgca aggcccgact atccagctcc tggaccaccc atctgcgctt caatgcaagc
     3721 tgaagcaatg cggctccgag ggcaccggcc atgtgcggcc gccgctcact ccaatccacg
     3781 caggcgtagg cgaatcgccg ccgctgcgcg cgtgccgcat ccacattcac accgagcatc
     3841 gccagcgctc tcgccccttc cgccgacagc tcgtactcat ccgcgacggg agctgccgat
     3901 atccaacgca gctccctgag ccgatcatgc aggagcacgg cgatctctcc ggcgaggtgg
     3961 tcgtaacagg tccgcgcgaa ccggagtcga ttcggcgtgc gcggcacgaa tcgagcgcgc
     4021 ggctcgcctg cgatgaccat cagtgcctcg agcgcggcgg caaccttggg gccctcgagg
     4081 cgataatagc ggtgcttgcc ctgggccatt gcggtcacga gccgctcgcg cctcagtcgc
     4141 gccaggtgca cgctcgcggt ggaggccgat acgcccgcca cgatcgcaag ctccgtgctg
     4201 gtccgtgcgt gtccgtccaa cagacagtaa agcatgcgcg cgcgcgccgg ctcgccgata
     4261 caagcggcga cccgggagcc ggcaaggtcg gcgggatgtt ggtcagccat gctttgatgc
     4321 tagtcgaatc gtcggcgcga ggctatcggt gcaccggacc cgcatgcgta cacccatccg
     4381 cctgcggcgc aggctgcgcc atccccgctg gcgaggcagg ccaagggcga gctccgccaa
     4441 caagcggcaa attgcggccg tccaccggca aagcgttgcc agaatcgcag tgcgatacga
     4501 ttcggcatta cataattccg cagtccgcgt gtcagagcgt gagttgcagc gaagaagttg
     4561 gtcggcagcg ctgcgatttg tacgtaggaa agggatgccg gaccaacacg tatgcctgct
     4621 acatctacct aacggtaccg cggcgcgatc accagttgca caaagtgcga accagccgct
     4681 ttttttcccg ctcacacaca acactgtctg cggattcaga cattttgcat cttcacgatt
     4741 taagcgctct actgtctcac gtaacgcaag ggcaaacccg ccgcaaggcg gggacgcaaa
     4801 gcctccggtc tcgagagcct cgtgggctcg agatagcggg gttgccggca acagcacgag
     4861 catcgggtgt tgccgtcatg ccgatgacga tcgccgactc cgctcgcgcg ctcgcatcat
     4921 gcttcgctgc cctggcgacg gtccttacgc ttgtcggggt gggaatacat gcaagctttc
     4981 gcctttctcg ccggcgcgca tctgcgaaat gcgctgctgt gcgcggtggt cgctgcgagt
     5041 ctcgcggcgt gcggtggaga tgatgcaggg agcggagcgc aagccaccgg tctgcagcag
     5101 acatcccagg gtaccaatca cttctcaccc acgatcgaag gcgtccccgc cgtctcggtc
     5161 gatgtgggct cgcgttatgt cttcctgccg agcgccacgg acgcgaacgg cgcccctctc
     5221 agatattcgg tctccaacaa gcccctgtgg gccagtttcg ataccggcac cggcatgctg
     5281 tcgggcacgc cctcgcaggc cgatatcggc gacaccgtgg gcatttcagt catcgtcacc
     5341 aatggccagg gccagagctc gatcgggccg ttcaacctga gggtgcgccc gcatccggga
     5401 cggccgctgc cctcgccgca gccgacggag ccggtcacgg actcaccgcc gctgatctcc
     5461 ggcgtgccca tgtccactgt catcgtcggc caggtgtaca gcttcacgcc tcgggcgagc
     5521 gatcccgacg gggatacgct ttccttcacg atcgccaacc ggcccgactg ggccaccttc
     5581 gacagcagca ccggcagact cgccggcatc ccgaccgtcg gcagcgaagg aacattccag
     5641 cagatcgtga tcagtgtcag cgatggacag caggtgagcg ccctgccgcc cttctcgatc
     5701 caggtgatgc cgccagcggc acatacgccc agcatttccg gcatgccccc gacgagtgtc
     5761 gtcgcaggac aaacctacac cttctcgccc tcggccacgg atccggacgg cggcaccctc
     5821 accttcgaga tcgcgaacga gccgagctgg gccaccttca acagccgcac gggtcagctc
     5881 tcgggaactc ccaccgtggc agacgtcggt acgcgcgcag gcatcgtgat cagcgtgacg
     5941 gacggacagg cgagcgcgga gctccccgcc ttctccatcc aggtgctggc gagcgccaat
     6001 catccgccct cgatatccgg gagcccgccg acgagcgtcc ttgctggaac cgaatattcg
     6061 ttccagccct cggcgagcga tgccgacgga gacccactga ccttctcggt caagaatcgt
     6121 ccggcctggg cgacctttga tgcggccacg ggccggctct ccggcgtgcc ctccggcacg
     6181 cagaccggca aggatgccaa catcgtgatc acggtcagcg atggcgagac ctcggtgtcg
     6241 ctgccggcat tcactgtcac cgtaaccggc tcgcagcttg ccgcgcccag catctccggg
     6301 gcgcccacgg cctcggtcaa tgtaggctcg gcctatggtt tcactccgag cgcgactgct
     6361 ccgagcggca cctcgctcac cttcagcatc cagaacaagc cttcctgggc cagcttcaac
     6421 acaggcaccg gcgcattgac cgggacaccg gcggccggcg atgtcggaac ctatgccaac
     6481 atcattatct cggtgagcga tggtcaggcg agtgcctcgc tcagcgcatt caccgtgacg
     6541 gtcaaccaga tctcgagcag cagtgcgaca ctcgagtgga cgcccccgac cgagaacacc
     6601 gatgggacct cgctcaccaa cctggccggt tacaaggtgt actacggcac cagcgccggc
     6661 agcctgagcc atgtggtgca gctcgcgaac cctggactga ccggttatac cgtgagcagt
     6721 ctgtcctcgg ggacgtggta cttcgcggtc acggcttaca cctccggggg cgtggaaagc
     6781 agcctgtcgc cggtggtcag cgcgagcttc tgaggcacgg aagagggctc aggcctggcg
     6841 ccgcgcccgg ccgctgtgcc aatgggtgcg gccctccatc cagtaccagc ggagcagggc
     6901 cggggagccg aacaggatct cgcgcatgcg cttggccagt gacagtgcga tggccacatc
     6961 gttgccgatc ccgaacagct gtccgacgcc gaccagactg gcttcctgga ccccgagacc
     7021 ggcaggcacg agaaacagga aactgcgtgc agcctgactg aggctctcca gggcaagcgc
     7081 cgcggggacc ccgacgggcc gcccgaacca gcgcagcacc agccaggttt ccacggtccc
     7141 ggcgatcagg ccgctcagtt gccacagtgc ggttgccccc aggcgccacg gatgcgcgta
     7201 cagccgtgcg atcgccgcct cgagggctgc gggattgtcc ccggccaccg tgcgaaatcg
     7261 ccggcccagc atccattcgc ccagccgggc cagtcttgcg aagaccgcac caaaacgcac
     7321 caccgtaatc agaatcgcga tgagtggcag gctcaccagc agtgccagga ccagctccga
     7381 gccgagtctg gtgggaccgg ccgccgtgac caggcagagc accccgagga ccgcaaacat
     7441 gtactgtgcc gccacggtca gcacgacctc cacgacaata ctggcggcgc caacgggcgc
     7501 aggcgtcccg gattgcgcca gcagcctgac accaacgatc tcgccgccta tgtttgcaac
     7561 ggggagcaac ctgttgaatg cctcgcgcac ggctgcgatc cacagcaggg tgctcagtcg
     7621 cgtctgcggc ggcaggagca gtctccatcc gagcacatcg agcacaatag gcagagtgtg
     7681 gagcgggacg atccacagca acacggctcc ggcgcccagc aatggtgtga tgatcgatgc
     7741 ggctccctgg cgcacgatca aataggtcaa cagcgcgagc ccgaggatga aggcggatcg
     7801 gattgcgaat ctcaagctgc ggcgctcccg ccgggctccg gctgcgagcg catgcgcaac
     7861 aggtcggcat atccgccgcg gctcgcgcca gttgcctcca tggcggcgcg tacccgggga
     7921 ctcagcaagg ctgcgagctc atccgcatga cgatacttgc gcatgccgga gctgatcggc
     7981 cctgaagcgg atagcgccgg atgcaggtag atctcggtcg tcccccgagg caatttggcg
     8041 agcgcatgta agagagtggc ttcgccgagc gagccgctgg cggccaggcc taacacctgg
     8101 tcgttgcagg cgatgtcccg cgcgcgcaga cggcgtctca tcaatgcaag ccagggggcg
     8161 agcagcaggg ccgctgcgcc cctccagagc gtgttgccgg aggcagccag agcgggcggc
     8221 tcgtagggca cccgcatcgc agccagccca aactcggttc cgacccgcag aatgatctcc
     8281 agcagcgtcg ggtgcagatg gaagtgcttg tgtgcattca cgtgatcgag cgccaggccg
     8341 ctcgaggcaa acgcctcgaa ttgcgcgcgg atctctgcct cgagctgttt gcgcaccgcc
     8401 ggcagcgcaa agtaacgcag cccttccacg aacatccggt tcggaaagcg gccttgccga
     8461 tttaccaggg cagggatcag tcgatggggc agaaccgacc agccgtcggc taacactagg
     8521 tgcagcccga tgcgcagacc gggcagagca cgcgctcgag cgattgcatc agcgcaggcc
     8581 ggcgcgccga ccatcaggct cgctgtggtg agtacgccgg atacactcgc ctcgtcgacg
     8641 gccgcattga tggcttcgtg caggccgaag tcatccgccg tcacgatcag gaacttttcc
     8701 atgacggtct cggcccgtac cggccggtac gctcccccgc cggggttcgt tcaggcttcg
     8761 cgagcgcgca ggaagcggaa gaattccacc ccctcacgca ggcggcgccg ggtgagatcc
     8821 cagctcgtga gcatctcggc ggtgagctcc gcgatcttgc gcggccggaa atagaaattc
     8881 cggtagaagg tctccacgcc gtgatagatc tcctgtgcgc tcaggtgcgg atagctgatc
     8941 ggagagagct ggatgccctg gctgttgacc aggttgaggt cggctttctc ctcgagccag
     9001 ccctgctgca ccgcctggcg gtacagctcg gtgcccggat agggcgcggc cagcgagacc
     9061 tggatcgtat gggggttgat ctccaccgcg aagcggatgg tctcgcgaat cgtctcggct
     9121 gtttcaccgg gcagacccag aatgaacgtg ccatgcaccg tgatgcccag cttgcggcag
     9181 tcggcggtga atcgccgggc gatgtcggtg cgcaggccct tgcggatatt gcgcaggatc
     9241 tggtcgtccc cggactcgta gccgaccagc aggagccgca ggccattgtc cttcatgacc
     9301 tgcagtgtct cgtacgggac attggccttc gcgttgcacg accacgtcac tccgagcttg
     9361 cccagccctc gcgcgatctc ctgcgcccgc ggtcggaaat cagtgaacgt gtcgtcgtcg
     9421 aagaagatct ctttcacctc gggcaggttc tgttggatcc agcgaacctc ctcgatgacg
     9481 ctcgccggac tgcgcacccg gtatttatgg ccgccgacgg tctgcggcca caggcagaag
     9541 gtgcacttcg agcggcaccc gcggccggtg tagaaggaca cgtaagggtg ctgcaggtaa
     9601 ccgatgaaat agttgctgat cgtgagatcg cgcttgtaca ccggggagac ccagggcagc
     9661 gcgtccatgt ccgcagcggg cgggcgggca tcggtatggc ggatgctgcc atcgggagct
     9721 ttgaaactca ggccggcgat gctctgcagg tcgcggccct cggcgacctc cgcacaggtg
     9781 tagtcaaact cctcccgtgc aacgaagtcg atcgccgctg aggcggacag ggagccaatc
     9841 ggatctacgg ccaccttggc tccgatcatg ccgatcagca ggtgcggatt ttccgccttg
     9901 agacgttccg ccacgtgcgc atccgtcggg aaggacggtg agctggtgtg catgacgatg
     9961 aggtcgtagt cgcgcgcgag gggcaggatc tgttccaggt tgagcccatc ggccggcgca
    10021 tcgagcaacc ggctgcccgg taccagcgcg gcaggctgag cgagccaggt cggataccag
    10081 aaggagcgga tctcgcgctt ggcctgatag cggctgccgg cgccgccatc gaagccatcg
    10141 aaggaaggag ggtgcaaaaa aagcgttttc atcatctgcc catgcctcgc cgcctcagca
    10201 gtcatacgat tggatgcagg gaaccgtcgc gggccacccg atagcggatg tcgcgccaat
    10261 gcacgctgct ggtgacaaac ccccagcacc ataacccaaa cgccagaaag tcgctcagcg
    10321 gcagaaccca ggggctggca gtacgcagtc gcggcttcca cgccacggcg tgcaacatga
    10381 agcgcgccag gatcgtgagc gccagcatga gaagtgagag gtccgagccc ttcgccagaa
    10441 tggctcccgc cacggccagg ggaacgccaa agctgatgaa agcgaaagtg tagcccaaag
    10501 gcctgacagc tcgaatcgtc cgcaaccatc gcagctcgtg ccgcacgagc tcgcccaggc
    10561 tgcgctcggc gatgcacgtc tcgacgacga catcggagag caccgtgcgc aggccgacgc
    10621 gccgggtcag ctcgcccagc cgataatcgt ccgccagctg atccgcgatg gcggcgaagc
    10681 cgccgacccg cgtcagcgcc tgccggcgga tggcgatcgt cgcgccgaag gcgaaggcgc
    10741 gcgagccgaa cagggccgcg atccgcgccg aaggcatgaa ccagtcgttg acgaacaccg
    10801 aggcgaggag ggaccacagg ccgggacggg gccgcccaac ataggggcag gtgacgatcc
    10861 cgactcgcgc atccagtagc ggatgcacca cccgcgccag atagtcgggc gcgacgcgca
    10921 cgtcactgtc cgcgatgacg aggaactcgt agcgggccag cggcatcatg ttgatcagat
    10981 tgctgacttt acggctgctg ccacgctgag gcgcatcggt cgcgatgacg aggtcgagtt
    11041 gtgggaactc ggcctgcagg ttacggacga cctcgatcgc cggatccgcc gtatctcgaa
    11101 ccccgaagac gatctggaat ttcggatagt cctgcctgca gaaggatcgc aggtactcaa
    11161 gcagatccgc ctccgcgccg cacagcggct tgagaaccgt caccggcggc tgttccggcg
    11221 ctggcgtctc cggtcgccgt gggccgggtc gaagcaggca gatcgccagc caagccatga
    11281 ggctgtagcc cagggccagc accgccagaa acagcccggc cgccattggc cacgtcatcg
    11341 ggtcacccac acgcgctcgg tggcggcaag ccccattcct gctggggccg agccgtgagc
    11401 cggcgagcaa caagccagag aaccgtgatc agatgggccc acgtcatgct ctccgtcgat
    11461 tttgcgacct gagactttgt attacaaagt gtagcacggc ttcgagtgca actcgtgcac
    11521 accgtagcca ggagccgtac aggaagttgc ctacctttcc agtaggaaag ctttgcgctc
    11581 gcgagacgga gcaccggcgc atgcggccag cgagcttccg accccgacca atctcttttg
    11641 ccgtgctgag tctgacctgt cactcgagcg cacgtaccgt gcggctgagt gtgtgcggca
    11701 tgcgccggga cgtgcagggc gagcggctga aatgaagcct cgcgggcgcc cccccgtgta
    11761 tagtccgcaa ccttccgtga gccaacacgt tcgggggact ccatgcgttg caagggaatc
    11821 gcgagtgccg ttctcgccct tatcagtatc gcgctgtgtg gcactgcgtg gtctgcttct
    11881 ccgttgttgc tgcggaatcc ctcgctgggt caggacagga tcgcattcct gtatgccgcc
    11941 gatgtttgga cggtaccgcg cgagggtggg gaagcacaac gcctcacctc tggcgctcat
    12001 gtcgtcgagg gtccctatta ctcgccggat cagcagctca tcgcctattc gacttcgcag
    12061 cacggacaga tcgatgtgta cgtcatcgcg gccgacggcg gggttccgcg ccggctcacc
    12121 tgggagccca ccggcaatca cgcggtcggg tggacgcccg acagcaagga aatcctgttt
    12181 acctcgcagc atgccagcta ctccgatttt ccccgcctgt ttcgcgtgcg tgccgatggc
    12241 gtcggctccc cgcaactggt gcctctgccg agcgtcgact ggggaagcct ctcgagcgat
    12301 ggcaacacgc tggcctacgt gccggtcatg cgctggcaga acgcgtggaa gcactatcgc
    12361 gggggtcaga ccacgcccat ctggctggtc aatctcaaga ctctggacct ggagaaaatc
    12421 ccgcgcgaga actcgaacga ctccaatccg gtctggagcg gcaagaccgt ctatttcctc
    12481 tctgatcgca acggtccggt gtcgctcttc agctatgaca ccgacagcaa gcaggtgcag
    12541 ccggtcgggg agaatcacgg cttcgatctg aagagactga gcgccggacc cggggcgctc
    12601 gtctatgagc agtttggctc cctgcatctt ctcgagctgg ggaatcatca ggatcgtgtc
    12661 gtgcccgtca cgattcgcgg cactctgcct gagctcgctc cgcacctggc gaaggtgccc
    12721 gcgaagggcg tggataacac ggcaatctca ccgaccgggg cacgaatcgt cgtcgaagcg
    12781 cgcggggaca tcttcaccgt gcccgcagag aagggcaaca ctcgcaattt gacgaacacg
    12841 cccggcgcgg cggagcgcga ccccgcctgg tcaccggacg gtaagtcgat cgcctacttc
    12901 agcgacgctt ccggcgagta ccagctctat atccgcgacc aggaagggct gcggcccccg
    12961 aaggtcatcg acctcggccc ggacccctca ttcttctacg ccccgcgctg gtctcccgat
    13021 tcgaaacata tcgcctattc ggacaaacat ctgcggctgt ggtacatcga tgtctccggc
    13081 ggcaggccgg tgaagatcga cacagcgctg cgtggcgggt tcggcaacaa caccgagctg
    13141 tcctggtcgc cggactcgca atgggtggcc tactcgcgcg acctcgagag ccagatgcgg
    13201 gcgatcttcc tctactcgct cgctacgcac agctcgcatc aggtcaccga cggcatgagc
    13261 aatgccgatc atccggtttt cgacccgagc ggcaagtacc tgtatttcac cgcctcgacc
    13321 aacaacggtc catcggatgc cggcatcgat ttgtcctctc tggaccgcgc caccaacagc
    13381 agtgtgtacg tcgtcgtcct gtcgaagacc ggtgcctcac ccgtgccccc ggaaagcgac
    13441 gacgagaaag gcaaggatga caagggtaag gacgagaagg gtaaggacga gaagggcaag
    13501 aagggcgaca aggacgatga ggattcctcg aaggacgagc acgcagaggc cgacaagagc
    13561 ggctccgacg aaaccgactc gaaaaagtcc tccggcaaga aaaaggagga gaaagccaag
    13621 ccgacggtgg tcgatctggc cggtatcggc aatcgaatcc tggcgctgcc cattccagcc
    13681 cggaactacc agggcatttc ggtaggcaag accggggtgt tgttcctcgc ggagggcgcc
    13741 ccggttggcc gtccgtccgc acccgatgag attcccatcc atagtctgtg gaagttcacc
    13801 accgagaagc gcaagaccga agaggtgctc agtgagctga gtgcctatgc cgtgtcattc
    13861 aatggtgaaa agctgttcta tgcgcggcac gatagctggt tcatcactcc ggtctccgat
    13921 ctcaagcctg gctcaccgga gggaacgccc ggcaaaccgg tggatgcgaa cggcatgtgg
    13981 gcgatgcgcg atccgcgcgc ctcatggaag cagatgtttc atgagacctg gcgtatcgag
    14041 cgcgatttcc tttacgaccc gcattatcac ggcctcgacc tgcagaagat cgaggcccgc
    14101 tatcagccgt atgtggccgg gctggcttcg cgcgccgaat tcacctatct gagcgaggag
    14161 atgctgggcg agattcagct cggacacgtg ttcgtgcgcg ggccgccagc accgccggat
    14221 gcacccaagc ccggattgct tggcgcggac tatacggtgg aacacaaccg ctatcgcttc
    14281 gccaagatct acaacgggca gaattggacg ccctcattga cggcgccgct cacgcagcct
    14341 gggatcaaca tcgtggaggg cgactacctg ctggcagtca acgggcgcga gctgtacgcg
    14401 acggacaatc tcgacagctt cttcgatggc accgcgggca agcagacggt gttgcgtgtt
    14461 ggcaagaagc cggacggaag cgatgcccgc gatgtgacgg tcgtgccggt cgattccgag
    14521 catgggttgc gcaatctcga ctggatcgag tcgaatcgac acaaggtgga ccagctctcc
    14581 ggcggcaagg tggcctacat ctggatgccc aacaccgccg gggccgggta taccaacttc
    14641 aaccgctact tctacgcgca gctcgacaag caggccgtcg tgctcgatga gcgctacaac
    14701 gagggcggtt tcatcgctga ctacatcgtc aatgtgctcg gccagaagcg tctgagcgga
    14761 gcgattgggc gtgaaggcga gccggtgcac gacccggagg gagcgatctt cgggcccaag
    14821 gccatgatca tcaaccaatc cgccggctcg ggcggggatg ccatgccgtg gtacttccgc
    14881 aaagccaagc tgggaacact ggtcggcacc cgcacctggg gaggtctggt tggaatcggc
    14941 gggtatccaa acctgattga cggcggagca gtgacggccc cgcgctatgc catttacgga
    15001 ttgaatggcg agtgggaagt ggagggtcat ggcattcctc ccgacgtcga ggtcgaggtg
    15061 ctgccgaagg aggtggccgc cggccacgat gcgcagctcg aacgcgccgt ggcgatcgtg
    15121 atggagcagc tcaaggagca tccggtggtc gagccccgca tccccgccta tcccaactac
    15181 catcagcagg acgcgctcgg cgcccactga tcagtgcaga ccccacagcg caccggcagc
    15241 cgcaagcttc tggtgcgctg cccagggccg gaaggtgccg cgacccgcac gggtgagcga
    15301 tcgattgagg gctgcgacgg aggtgcggtg caggtccgcc aggcgggcca ggatgtcccg
    15361 gtcgctcaac acctcgaacg cgatgccggc ctgcttcagc tcggaggtca ccacttcacg
    15421 gtaaagctga gcttcccggg cgtgcgcctg cacgtgcgga ttatggatgc gcccggggtc
    15481 ggtgaggctg ccgccgacga cgccggctgc ctcgatgggg gcaagatcgg ctagcgcggc
    15541 tctcatctca cggcgtgccg tcatgcgcac cgcggccacc gccttgcgac tctcgactat
    15601 agcggcgcgg ccctcgagct ccacggtcgg gtgaaaggga aagcgcgcga acttcccgcc
    15661 ctgcgggctg agtggcacct cgcaactggc gagcacctgc caggactcct tcgcgccttc
    15721 gacgagaacg cacagtgtca cgccggagcg gacccgaaac cccacgactc tcattgatcg
    15781 gtccccgcaa cttccgggcg tgatcatgat acgcagcccc ggggccgttt cattggtgac
    15841 cgaaatgctt gcgccgccac gcggcgggcg gcgagccgaa ttcccgcctg aaggcccggc
    15901 tgaatgccgt atcggtctga tagcccacct cctgcgcgat ggtcgccagc ggagcgctgc
    15961 tgcggcgcag aagattggcg gcgaggagca tgcgccactg ggccaggtat tgcatgggag
    16021 cgctgccgac caggtgctgg aatcgctcgg ccagcacgga gcgtgaagca cctgccttgc
    16081 gggccagctc ttccagggtc catgcatggg cgggatcctt gtgcagcaag gtgagtgccg
    16141 cgcccacgat ccggtcgccg acacccgcga gccagcccgt tccgccctta ccctgctcgc
    16201 tcatgtacag gcgcaggacc tcgatgaaga gcacctcggc cagcttggcg agcacgccgg
    16261 cgccgccggc ccgcggagag cgcgcctccg ccagtgcata gcggacagat gcctcgagcc
    16321 acgttccggc atcggacccc cggacattca ctttcacgat cggcggcagc cctgcgagca
    16381 gcatgcgggc gaggcgggca tcgcaagcca ggtagccgca cacgagccgc gtcactgcac
    16441 ctccgccacc ataggccagc tggcgtgggc gacgggcgag cacggcatcg agtcggctcc
    16501 cggtggccgg tttgagtccg ggctgcgagc tcatccggtg ggcgtggcca tccggaaaga
    16561 caaccacatc gcctggcttc aattgcagcg gcggctcgcg cccgagctcc acgtaacatt
    16621 ccccttcggt gaccagatgg aagatcacca cacgctcggc gccgggctcg aggagcgggg
    16681 cagcgaagtc ggcccgcggc gattgataac accatggtgc ggtgaagcgg gcattgatga
    16741 agatggcgcc gacgagctgc accacgcgca aggtttgcga caacgcgtcc atcatttcag
    16801 cggcaggcca tcaagcacac aactcaatgc gggctgcccg gcagcgctgt cactacgtgc
    16861 tgatgtcgat ccgcagcccc accggcatgg ctgcttcgag cgcacatgcg ataggcgagt
    16921 tgcggtagcc atcctcgacc agcgagcgga gctgctccgc agtggcccct tgcgcggcga
    16981 tcctcacggt cagctccaga tcacgcgggc cggcagggac ctgctctccg cgctcgttga
    17041 tcatgcccag caatccccga gtgtccgagc ggctgctggc cacgatctcg agccgctgca
    17101 gctcgatccc tcgcgccgcc gctcgcaggg cgatcgaggt cgccgtgcaa gctgccagac
    17161 ccgcgcgcat cagccagccc ggcgtcacgc ggtcacccgt gccgcccagc tcccgcggca
    17221 tgtcggttgg aatctgcgtg ccgttgagat gacccgcgac gacccgcaga cccctctccc
    17281 agcggaccgt tgccggggca tcatcgtgaa gacctgctcc ggggcggcgc tgcaggaccg
    17341 attccacccg caacagtgcc gtcgcgacgt tctgagtggt catgcgcgct cctgtgtaca
    17401 cgaactccag attgtcgtcg ctgagcatcg cactgcctag aggcgggtgg ccgcagagtc
    17461 gtcgtccgga tgggcgagca gcaattccgg acgccaggac agccactgtc gcatggcgat
    17521 tgctgcccgg atgatcgggc agctgagcga gatgctggga caggacacca gcccagtagg
    17581 tcgccagagt tcgcgcctcg ggactcagag gtggcgacga tggatgcacg actgcaaagg
    17641 cgaatccagc gctatggctg ggatctggcc gcgagcgact atgaaccgct ctggaaggca
    17701 cagcttgccg aggcccaaaa ggcggtgctg cgcgccgcct cgcccgctcg cggcgaacgg
    17761 gtgctggatc tcgcttgcgg cacagggctg gtggcgctgc aagcggcacg agccgtcggt
    17821 ccgcacggcc gggtcctcgg catcgatctc tcggcgcgaa tgattgcggc cgcccgcgcg
    17881 catgcactcg agcaggcggt gccgaacgcc gaattttcaa ggatggacgc cgagaagctg
    17941 gatcttcctg cgcgcacctt cgacgtggcg ctgtgcgcgc tcggcttcat gtacctgccg
    18001 gaccctgaac gcgctctgct ggagatgcga cgtgtgttac gtcccggtgg ccgcatcgcc
    18061 ctggcggtct ggggggagag agagcgctgc ggatggtcgg gtgtctttcc gatcgcggct
    18121 gcggaagtcg ctagcgacgt gtgcccgctg ttctttcagc tcggacaggg gagcgcattg
    18181 gccgagctct gcgggcggtt gcagttcgat acgatccatc attctagaat tcaggccacg
    18241 ctgagttacg cggacgctgc tacagcatgc gacgcggcac ttgttggtgg tcctgttgcg
    18301 ctggcgtggt ctcgcttcag tgaggaggtc agagatcgcg ttcgagcacg ttacctgcag
    18361 tcgatcgagc cctggaagtg cggcgccggt taccgtgtac ccgctgagtt tgtcgtggtg
    18421 agtggggtcg tgcctcagcc gggtggcctg cgcattgcgg ccggcggttt ttccgtgtga
    18481 ttggccgagg tggcagctat gatgtcgtac cggcgcgagc cggcgtgagg gctgcaaccg
    18541 atgcgcggca gcggcccgca gagatggcac cttgccggag ctttctcgat gtcgatcccc
    18601 acgcatcgcg gcggcctgac tcacctgact caccaggcgg tgcaggagcg gggccccgag
    18661 cagcactcca tcgggcatat tcccgcgagc gagcggcatg g
//
LOCUS       DASRHN010000364         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1288499, whole genome shotgun sequence.
ACCESSION   DASRHN010000364 DASRHN010000000
VERSION     DASRHN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288499"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VFB37_14740"
     CDS             <1..1043
                     /locus_tag="VFB37_14740"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HZO23757.1"
                     /translation="RSCTERSGGHINPEFLRSQLELSTRVCHSIAEARADLVRLRSII
                     VEVAREHGLAPIAASTHPFGRATRQMPTEREQFFALAREMQAAARRLMVCGMHVHVGI
                     DDDDLRVDLMNQLSYFVPHLVALSCSSPFWEGERTGLMSFRLSLFSSLPRTGLPERFS
                     SYGELRRHLDMLIRNGLIENASKMWWDVRPNPRYPTLEARVMDCCTSIDDSMCLTALV
                     VCLTRMLYRLRRSNQSWRWYKNVLIAENRWRAMRYSYDEQLLDLARGQLAPFAEIAEE
                     LIGLLREDAEALGCLRQVQDVRTILSRGTSAHRQIRTYEEARAAGASEREALRAVVDF
                     LIRETAELGGVA"
     gene            1040..2506
                     /gene="xylE"
                     /locus_tag="VFB37_14745"
     CDS             1040..2506
                     /gene="xylE"
                     /locus_tag="VFB37_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HZO23758.1"
                     /translation="MIDQQGGRASEEVNTRLVVTLTFVATLGGLLFGYDTAVISGAVS
                     SIDAYFIEPLNLAETARSSLSGWTIASALFGCIIGAAIAGWISTGLGRRGGLLIAGAL
                     FLLGSLGSAVPEIGFGPIGQMGPHALPQFIFYRILGGAGVGIASMLSPLYIAEISPSA
                     IRGRLVSFNQLAIVVGILVVYFVNWFVASRGNDMWLKSVGWRYMLASESIPALVFLVL
                     LLFVPDTPRWLVLRGRTAEALTQLQRVMGEGAARAIVADIERTLAVKTAHLFTFGVPV
                     IVVGILLSVFQQLVGINAVLYYAPLMFQNMGASTDTAFLQTVVVGAANLAFTFLAIVT
                     VDNWGRKPLLIAGAVIMAVAMLTLGCLFSAQSVGLAALVAVVVYIGGFALSWGPVVWV
                     LLAEMFPNSIKGKAMGIAVAAQWIANLLVSGSFKVLDGNSTLNALFHHGFAYWIYGAM
                     SVLAAVFVIRYVPETKGRTLEAIQDLWGGQQSAVADTR"
     gene            2525..3277
                     /locus_tag="VFB37_14750"
     CDS             2525..3277
                     /locus_tag="VFB37_14750"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZO23759.1"
                     /translation="MSGLVLISSAQGVCELRLNRPDKRNALTLAMYEALTQAMQQAEQ
                     DDSVRAILLGGEGASFCAGNDLQDFLSSTDLGEAHSAVRLLRLLPRIRKPLIAAVHGR
                     TVGFGVTLLLHCDLVLAGRGAQLSMPFVRLGLVPEAASSLLLPRLIGHQRAAELLFLG
                     SPLDAAEAFRLGLVNQVVEEERLPEEARALARSVAQQPTGALLATRRLLRGSPDEIDA
                     RITEELHAFRAQLRSEEFRTAAQAFFTKRRPE"
     gene            complement(3360..>3663)
                     /locus_tag="VFB37_14755"
     CDS             complement(3360..>3663)
                     /locus_tag="VFB37_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HZO23760.1"
                     /translation="MAATGAAAFGLRAGLSPVQAAGKELPHLTDADPTAKALGYTEDA
                     SKVDKTKFPSYKPGAMCGNCNFFQGQAGEAYGPCQIFAGKSVNTKGWCASHTPKAK"
BASE COUNT          550 a         1155 c         1242 g          716 t
ORIGIN      
        1 tgcgctcctg caccgagcgc agtggcggtc acatcaatcc cgagttcctg cgctcacagc
       61 tcgagctgag cacgcgtgtc tgccactcga tcgccgaagc gcgcgcggat ctggtccggc
      121 tgcgctcgat catcgtggag gtggcgcgcg agcatggtct ggcgccgatt gccgcctcga
      181 cccatccctt cgggcgagcg acccggcaga tgccgaccga acgggagcag ttcttcgcgc
      241 tcgcgcgcga gatgcaggcc gccgcgcgcc ggctgatggt ctgcggcatg cacgtgcatg
      301 tcggaatcga tgatgacgac ctgcgcgtgg acctcatgaa ccagctctcc tacttcgtgc
      361 cccatctggt cgcactgtcg tgctcgtcgc ctttctggga gggcgagcgc acgggtctga
      421 tgtcatttcg tctcagcctt ttcagctcgt tgccccgcac cggcctgccc gagcgcttct
      481 cgagctacgg cgagctgcgg cggcacctgg atatgctcat ccgcaatggc ctgatcgaga
      541 acgcctcgaa aatgtggtgg gacgtgcgac ccaatccgcg ctacccgacg ctcgaggcgc
      601 gcgtcatgga ctgttgtacg agcatcgatg actcgatgtg cctgacggcg ctcgttgtgt
      661 gcctgacgcg tatgctctat cgtttgcgcc ggagcaatca aagctggcgc tggtacaaga
      721 acgtgctgat cgccgagaac cgctggcgag ccatgcgcta cagctacgat gagcagctcc
      781 tcgatctggc gcgtggtcag ctcgcgccgt ttgccgagat tgccgaggag ctcatcgggc
      841 tgcttcgcga ggacgccgag gcgctggggt gtctgcgcca ggtgcaagac gtgcgcacca
      901 ttctcagtcg cggcacgagc gcgcatcgtc agatccgaac ctacgaggaa gcccgcgccg
      961 cgggcgcgag cgagcgggaa gccctgcgcg ccgtggtgga cttcctgatt cgcgaaacag
     1021 cagagctcgg gggggttgca tgatcgatca acagggaggg cgcgcctctg aggaggtcaa
     1081 cacgcgcctg gtcgtcacgc tgacgttcgt ggcgaccctg ggagggctgc tgttcggcta
     1141 tgacaccgcg gtcatctccg gagcggtcag ctcgatcgat gcctacttca tcgagccgct
     1201 caacctggcc gagaccgcac gcagcagcct ctcgggatgg accatcgcca gtgcgctgtt
     1261 cggttgcatc atcggtgccg ccatcgcggg atggatctcg accggtctcg gacggcgggg
     1321 cggactgctc atcgccggcg cgcttttcct gctcggctcg ctcggctccg cagttccgga
     1381 gatcggcttc ggcccgatcg ggcagatggg gccgcatgcg ctgccgcagt tcatcttcta
     1441 tcggatcctg ggcggggcgg gggtcggcat cgcctccatg ctctcgccgc tgtacatcgc
     1501 ggagatctcc ccgagtgcca tccgcggccg gctggtgtcc ttcaatcagc tcgccatcgt
     1561 cgtgggcatc ctggtcgtct atttcgtcaa ctggttcgtc gcctcccgcg gcaacgacat
     1621 gtggctcaag tcggtcggct ggcgctacat gctcgcctcc gagtcgattc ccgcgctggt
     1681 gttcctggtg ctgctgctgt tcgtcccgga taccccgcga tggctggtgc tgcgcgggcg
     1741 cacggcggag gcgctcaccc aattgcagcg cgtgatgggc gagggggccg cgcgggccat
     1801 cgtcgccgac atcgagcgca cgctggcggt caagaccgcg cacctgttca ctttcggagt
     1861 gcccgtcatc gtggtcggca ttctgctctc ggtcttccag cagctcgtgg gcatcaacgc
     1921 ggtgctgtat tacgcgccgc tgatgttcca gaacatgggc gcctcgaccg acacggcgtt
     1981 cctgcagacg gtggtggtgg gcgcggcgaa tctcgccttc acgttcctgg cgatcgtcac
     2041 cgtggataat tggggccgca aaccgctgct gatcgcgggc gcggtcatca tggcggtagc
     2101 gatgctcacg ctcggctgcc tgttcagtgc gcagagcgtc ggactcgccg cgctcgtggc
     2161 tgtggtggtg tatatcggcg gcttcgcgct gtcctggggc ccggtggtct gggtgctgct
     2221 ggcggagatg tttcccaact ccatcaaggg caaggccatg ggcatcgcgg tcgctgcgca
     2281 gtggatcgcg aatctgctgg tgtcgggctc attcaaggtg ctggatggca attcgacgct
     2341 gaatgcgttg tttcatcacg ggtttgcgta ctggatctat ggagcgatga gcgtccttgc
     2401 ggcggtgttc gtgatccggt acgtcccgga gaccaagggc cgcaccctcg aagccatcca
     2461 ggatctgtgg ggcggacagc agtcagcggt tgcggatacc cggtgagtca cgcatgagcg
     2521 ctcgatgagc ggactcgtcc tcatcagcag cgcacagggg gtgtgcgagc tgcgcttgaa
     2581 caggcctgac aagcgcaatg cgctcaccct tgcgatgtat gaggcgctca cgcaggcgat
     2641 gcagcaggcg gagcaggatg actcggtgcg cgccatcctg ctcggcggcg agggcgccag
     2701 cttttgcgcc gggaacgatc ttcaggattt cctcagcagc actgatctcg gtgaggcgca
     2761 cagtgcggtg cggctgctgc gcctgctgcc gagaatacgc aagccgctca tcgccgccgt
     2821 gcacggtcgc acggttggtt tcggtgtgac gctgctcctg cactgcgatc tggtgctggc
     2881 aggccggggc gctcagctat cgatgccgtt cgtacgtttg ggcctggtgc cggaggcagc
     2941 gagctcgttg ctattgccac ggctgatcgg tcatcagcgg gcggccgagt tgctcttcct
     3001 cggctccccg ctcgatgcgg ccgaggcgtt ccggctgggg ctcgtgaatc aggtggtgga
     3061 ggaggagcgg ctgccagagg aagcacgcgc actggcaagg tcagtcgcgc aacaacccac
     3121 tggcgctttg ctcgccacgc gcaggttgct gcgtggaagc ccggacgaga tcgatgcccg
     3181 gatcacggag gagctgcacg catttcgcgc gcagcttcgc tctgaggagt tccggaccgc
     3241 cgcacaggcg tttttcacca aacgtcggcc ggagtgaggc ggggcggcaa tcgaacccac
     3301 cctgcgcggg gcgcccaagg caggacgcaa tcccgagcgg actcgaatcg gcaggtgagc
     3361 tacttggctt tcggggtatg agaggcgcac caccccttgg tgttcaccga tttccccgca
     3421 aagatctggc acgggccata ggcctcacct gcctgcccct ggaagaaatt gcagttgccg
     3481 cacatcgctc cgggcttgta gctcgggaac ttggtcttgt ccaccttgct ggcatcctcg
     3541 gtgtagccga gagccttggc agtcgggtcg gcatcagtca gatggggaag ctccttaccg
     3601 gccgcctgta ccgggctgag ccccgcgcgc agtccgaaag cggctgcacc ggtggctgcc
     3661 atc
//
LOCUS       DASRHN010000365         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1457796, whole genome shotgun sequence.
ACCESSION   DASRHN010000365 DASRHN010000000
VERSION     DASRHN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457796"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFB37_14760"
     CDS             <1..556
                     /locus_tag="VFB37_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23761.1"
                     /translation="EEIAGVLDGLDVLVSSFGPGPSGSAMSKDPAAAIADSISRADSL
                     RVAARALLSALAQRRPSLRLIVVGGAASLEIRPGVQFVDSGPDLAAALRQFGLPDAYK
                     SVMEAHRDAMNLFRISDRNWTYFTPAVMVMPGERTGRFRLGTSQPIVGADGQSRISAE
                     DYAIALVDEIELPRFVQRRLTIGY"
     gene            complement(618..1790)
                     /locus_tag="VFB37_14765"
     CDS             complement(618..1790)
                     /locus_tag="VFB37_14765"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO23762.1"
                     /translation="MLNHPLISCDDHLDLNQMPTDVWTSRFPAAMRSRAPHVEERDGR
                     AVWVCDGQSWGAWLGKASGSGGPKPLFTALDRGGADYTECRPCVPRLRLADMDRDGVF
                     AHVLFGPVTSIRHDDVELRDTCYSVYNDWLEEFCAVAPGRLLGVPMLPPAPESALREL
                     RRLAAKGVFKQANLQIAVVDPPIRDRRWEPLFQLLDETGMILAFHVTVFATVSNAFDP
                     LKGSPGATFLHAKMFVEQFLDPFVDLFAFGILERHPGLRIVIAESGAGWLPWVVEELD
                     YRHWRLWEAQEYWSDKGGIPLLTKPSELFKRQVYATFQQSPVTMRLLEFYGDNLLWAS
                     DYPHPDSIWPNSRRTIERTMGHLPGEVVRKITCDNAARLYRLNPQGGAAALHGDTR"
     gene            complement(1934..>2167)
                     /locus_tag="VFB37_14770"
     CDS             complement(1934..>2167)
                     /locus_tag="VFB37_14770"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HZO23763.1"
                     /translation="HAMTKSLRLLGFERVTLEPHSSHPVKLIVDPRLLASFDVAAGQW
                     RIEGGAYEITLSQAAGAPGESATVTLQGRLFGK"
BASE COUNT          384 a          694 c          704 g          385 t
ORIGIN      
        1 agaagagatt gctggcgtgc tcgatggcct cgacgtgttg gtcagttcat tcggccccgg
       61 tccgagcggc agcgcgatga gcaaggatcc cgcggcagcc atcgccgatt cgatcagccg
      121 agctgattcg ctgcgcgttg cggctcgggc gctcctgagt gcgctcgcgc agcgtcgtcc
      181 gtcgctccga ctgatcgtgg tgggtggtgc cgcgagtctg gaaatccgtc cgggagtgca
      241 gtttgtcgac tccgggccgg acctggctgc cgcacttcga cagttcgggc tgccggacgc
      301 ctacaagtca gtcatggaag cgcatcgcga tgccatgaat ctgttccgga tctcggaccg
      361 gaactggact tacttcaccc cggcggtcat ggtgatgccg ggtgagcgca cgggccgctt
      421 ccggctcggc acgagtcagc ctatcgtcgg agccgatgga caaagtcgaa tttccgccga
      481 agattatgcc atcgcgctgg tcgatgagat cgagctgccg cgctttgtcc agcggcgctt
      541 gaccatcgga tattgattcg atggtcaggt gtccggcaga gcccaccacc aacgggccgc
      601 gaccctggaa gcgcgcctca gcgggtgtcg ccgtggagcg cggccgcgcc gccttgcgga
      661 ttgagcctgt acagacgcgc cgcattgtca caggtgatct ttctcaccac ctcgcccggg
      721 aggtgcccca tggttcgctc gatcgtccgt cgggagttgg gccagatgct gtccgggtgc
      781 gggtagtccg acgcccacag caggttgtct ccatagaact ccagcagtcg catcgtgacc
      841 gggctctgtt ggaaagtcgc gtacacctgg cgtttgaaca gctccgaagg cttcgtcagg
      901 agcggaatgc cgcccttgtc gctccaatac tcctgcgctt cccagagccg ccagtgccgg
      961 tagtcgagct cctccacgac ccacggcagc cagccggcgc cggattcagc gatcacgatc
     1021 cgcaggccgg ggtggcgctc caggatgccg aaggcgaaga gatccacgaa cggatcgagg
     1081 aactgctcca cgaacatctt cgcatgcaga aaggtggctc ccgggctgcc tttgagcgga
     1141 tcgaaggcat tgctgacggt ggcgaatacc gtcacgtgaa acgccaggat cattcccgtc
     1201 tcgtccagga gttggaagag cggctcccaa cgacggtcgc ggattggcgg atcgactacc
     1261 gcgatctgca ggttggcttg tttgaacaca cctttggccg cgagtcgccg cagctcccgc
     1321 agcgccgatt ccggcgcagg gggcaacatc ggcacaccca gcagtcgccc cggcgcgacc
     1381 gcgcagaact cctcgagcca gtcgttatag acgctataac acgtgtcccg cagctcgaca
     1441 tcgtcatgcc ggatcgaggt caccggaccg aagaggacgt gcgcaaagac gccgtcgcga
     1501 tccatgtccg caagtctcaa ccgcggtaca cacgggcggc actcggtgta atcggcgccg
     1561 ccccgatcaa gggcggtgaa gaggggcttg gggccacccg atccggaagc cttgccgagc
     1621 caagcccccc aactctggcc gtcgcaaacc cagaccgccc ggccgtcgcg ctcctccacg
     1681 tgcggggcgc gagagcgcat tgcagccgga aaccgactcg tccacacatc ggtgggcatc
     1741 tggttgagat ccaggtggtc gtcacaggag atgagcggat ggttcagcat gacgggcctc
     1801 gggggcagcg tctttcgggg ctttgcgcct cgcccgcaac agtgcggggg agtgtacttt
     1861 agaacagcta gcttgctaga tgttccccgc tggcccgccg cgccagccta ggctgccgag
     1921 ccggaactga agctcacttg ccgaacaggc gtccttggag cgttacggtg gctgattctc
     1981 caggcgcgcc agccgcttgc gagagggtaa tctcataggc gccaccctcg atccgccact
     2041 ggcccgcggc gacatcgaag gaagcgagca gccgtgggtc cacgatgagc ttcaccgggt
     2101 gactcgagtg gggctccagg gttacacgct cgaaacccag caggcgcagg ctctttgtca
     2161 tggcatg
//
LOCUS       DASRHN010000366         7686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1119101, whole genome shotgun sequence.
ACCESSION   DASRHN010000366 DASRHN010000000
VERSION     DASRHN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119101"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..857)
                     /locus_tag="VFB37_14775"
     CDS             complement(66..857)
                     /locus_tag="VFB37_14775"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZO23764.1"
                     /translation="MSTATTENRDVGVIRESRPVVVTRAESNDGPLSTQLRTLGLSVL
                     LWPAFTVAAAKKEPLDAALKALSQFDWIVFASRHAVAAVTERVASPPKGLLVAAVGQA
                     TAQVLKQRGWPVELLPSEANAAALVSAFATRPIRDAKVLFPASSRALPTIATGLRQLG
                     AQVMQVEAYRTDGAPLDVEDCRAWIARDGIAAVTFASPSAVIELEQALGRKDFERLLV
                     GARAVAIGPTTARELLDRGCTAVLAESATLAGLAQTTLRLLQTRH"
     gene            complement(854..1810)
                     /gene="hemC"
                     /locus_tag="VFB37_14780"
     CDS             complement(854..1810)
                     /gene="hemC"
                     /locus_tag="VFB37_14780"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZO23765.1"
                     /translation="MSERTLRIGTRGSALALWQAHHLEELIRAQPDPPPVELVRIKTE
                     GDVRVDVPLWQIGGRAFFTKEIDKALLSNEVDAAVHSLKDMSTILEPGIELAAVLKRE
                     DPSDALVSRDGRRLAELGEGARVGTSSLRRRAFLARARPDLRLLELRGNVPTRIERLE
                     RGEYEAIVLAAAGLRRLGLEGHISELLPADIFPPAVSQGAIGVCVRTEDAEAARWVRR
                     LDDPATRLATTAERALLRTIEGGCQVPLGALGTLKDQALQLHATVCALDGSASLRAQG
                     GLAASLVNATRLGEQIAQELLRQGAARLIASERAARNQVEEP"
     gene            complement(1807..2535)
                     /locus_tag="VFB37_14785"
     CDS             complement(1807..2535)
                     /locus_tag="VFB37_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO23766.1"
                     /translation="MSTPAAQNRILVVEDDPHLAAGVAENLRAEGYDVVALGDGKPAL
                     EWLQAQQCSLIVLDVMLPEMDGFTLCRTLREQGNSTPVLFLTARGDPADRVRGLEAGG
                     DDYLAKPFHLEEFLLRVRAILRRWDWYRSTSSTPAGAVLQFAGNEVDFRAFRARSWNG
                     VAQELTEKEAMILKVLAERPGEIVSREDLLERVWGYDVFPSTRTVDNFILRLRKRFER
                     DPANPRHFLTVWGVGYRFLSEGEP"
     gene            complement(2532..3614)
                     /locus_tag="VFB37_14790"
     CDS             complement(2532..3614)
                     /locus_tag="VFB37_14790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO23767.1"
                     /translation="MKGTRVFQLTALALVVVAVIQVGWWLFDQRSYAIEKVRTTRELY
                     FQQLSAAQALLDSGASPESVRAKLPDVVIEGKHASLSPDVDRELLTEKDRRIRQYAWE
                     GGFFLLALAVCIGVIARALRAEALVRQEQESFLALVSHQFKTPLASLQLSLETMAMRS
                     LSAEQSRTLIDRMLADLARMAAMVTQILESVRLERGRVDLRREPLELSGAIARVVGQL
                     DERARKERITISTDIERGLYVLADPLALDVVVRNLIENAIAAITPVGGGTITLGARRL
                     DGEVELSVKDSGVGFRPADGARLFEKFTRLHPGGGNNYHGTGLGLFIVRRLMQLAGGR
                     VSAHSGGVGQGAQFVLVWPTAPAGPA"
     gene            complement(3611..4903)
                     /gene="hemL"
                     /locus_tag="VFB37_14795"
     CDS             complement(3611..4903)
                     /gene="hemL"
                     /locus_tag="VFB37_14795"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZO23768.1"
                     /translation="MTEHPHSRDLYERACRVIPGGVNSPVRAFRAVGGTPLFIQRGAG
                     QKIVDADGRTYVDLVGSWGALILGHAHPAVVAAINEASRLGTTFGAPCAAEVELAERV
                     VGSYPGIEQVRFVSSGTEAVMSAIRLARAFTARDLIVKFSGCYHGHADHLLVAAGSGL
                     ATFGRPSSAGVPEAFTACTRVLPLDDEDAVEALFSREGQRIAAVIIEPVPANHGLLLQ
                     RKAFLQKLRALTERHGTLLIFDEVISGFRLARGGVAQQTGITPDLATFGKVIGGGMPV
                     GAFAGPRPIMQRLAPLGDTYQAGTLSGNPVAMAAGITTLDILIRESGWERLESLGMEV
                     ERALKPVLAEAPFPIHLVRSGSLFWLSLHEGGAPRAAAPLDEKAGAHFSTLFHAMLDR
                     GIYLPPSAYEACFLSLAHTSADVLQLAQALRDSLAAIA"
     gene            complement(4900..6273)
                     /locus_tag="VFB37_14800"
     CDS             complement(4900..6273)
                     /locus_tag="VFB37_14800"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23769.1"
                     /translation="MHQIGVVGLSYRHANVDEVARFAVPKGEVAARLPSLRDSLGVSE
                     VFYVGTCNRVEVLYATLEGVPAGDARPAVFRALIGRDPEPGEANRVLRAWTGEAAVEH
                     LFLLACGLDSAQTGEQEIAAQLRGAWESARVARTCGPTLDRVLSEALGMATRVHRIEA
                     KQARAPSLGDMAAERVLRHLGQRPPPADKSIVALVGVSPMTRRCGAALHRAGIPLLVV
                     NRTLQAAEELAKLFSGRALTLDEFRIRPPAVGAVVLAAGGGERVLDAAALANIAKARI
                     GPVLGPGLEAGLRAGQAPPGLPSGGAPLLVDLALPPNADPEASRALGLLRVGMDDLIQ
                     AAQERRLAQLMRLAPVRAAIDDRLNHLRSELAARAIGRRLADLRGAFEQIAAEEAQRA
                     LEAEFSDLDENQRALLQRFASTVARRLAHLPLAGLRAAAVHASSETVEAFFREARLRR
                     RNNGRNP"
     gene            complement(6403..6672)
                     /locus_tag="VFB37_14805"
     CDS             complement(6403..6672)
                     /locus_tag="VFB37_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23770.1"
                     /translation="MNAKNGDLGSGTGGSANTDQVKDHLRAAKDAAAQAARARAAQAQ
                     DWARSQLDELQGRVEARPFAASGIALGIGFIAGVLVMGLVRSGRR"
     gene            6857..>7686
                     /locus_tag="VFB37_14810"
     CDS             6857..>7686
                     /locus_tag="VFB37_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO23771.1"
                     /translation="MSRQPPNHSDPLADAQRLVAHDDETYAFEAVAPPIVQSSLFTFS
                     SFEEMRDTFAGKRSRNVYSRTTNPTVTQFEAKMAELEQTDEAIGFPSGMAAISGTLLA
                     FVEPGDRIVCVRHVYPDVYRLFETLLRKLKVSVVYVDGADQAAVAEALPGAKVLYLES
                     PTSWMMQALDVRALASLARRHGALSIFDNSWATPVFQRPIRHGVDLVIHSASKYIGGH
                     SDTVAGVVAGRSDLIGVVRRTVCPYVGAKLAPFEAWLLVRGLRTLPVRMKAHEESALV
                     L"
BASE COUNT         1337 a         2705 c         2455 g         1189 t
ORIGIN      
        1 aaccatgcgt cgccagagat cggactgtcg cgtgcggcga ggtctgtgca atggaaatcc
       61 catgatcaat gccttgtctg caataaacgc aatgtcgtct gggcgaggcc cgccagcgtc
      121 gccgactcgg cgagcacggc cgtacacccc cgatccagca gctctcgcgc cgtagtcggc
      181 ccgatcgcca cggccctcgc gcccacgagc agccgctcga aatccttccg ccccaacgcc
      241 tgctccaact cgatcaccgc cgaggggctg gcgaaggtca ccgccgcgat cccgtctcgc
      301 gcgatccagg cccggcagtc ctcgacgtcc aggggcgcgc cgtccgtgcg ataggcctcg
      361 acctgcatga cctgcgcgcc gagctgtctg agaccggtcg caatcgtcgg caatgcgcgt
      421 gagctggcgg gaaacaacac tttcgcatcg cggatcggac gggttgcaaa cgccgagacg
      481 agggctgccg cattggcctc gctggggagc aactccaccg gccagccgcg ctgcttcaac
      541 acctgtgcgg tcgcctgccc gacggctgcg accaacaggc cctttggagg cgaggccacg
      601 cgctcggtga ccgcggccac cgcatgccgg ctcgcaaaga cgatccagtc gaactgactg
      661 agcgctttca aggctgcatc cagcggctcc ttcttcgccg ccgccacggt gaatgcaggc
      721 cacagcagca ccgacagtcc cagcgttcgc agctgcgtgc tcagaggtcc atcgttgctc
      781 tcggcgcgcg tgacgaccac tggtcggctc tcacgtatga caccaacatc ccgattttcc
      841 gtggtcgcgg tgctcaaggc tcctccactt gatttctcgc cgcgcgctcg ctggcaatca
      901 gccgcgcggc tccctgacgc agcagttcct gcgcgatctg ctcgccgagt cgggtcgcat
      961 tcaccagcga ggccgcaagc cccccctgcg cgcgaagcga ggcagagcca tcgagcgcgc
     1021 agacggtggc gtgcagttgc agtgcctgat ccttcagcgt ccccagagcg ccgagcggaa
     1081 cctgacagcc gccctcgatc gtgcgtaaca aggcgcgctc cgcagtagtt gcaagccgcg
     1141 tggccggatc atcgagccgt cgcacccaac gtgcggcctc cgcgtcctcg gtccgaacac
     1201 acacgccgat ggcgccctgc gagacggcag gcggaaagat atccgccggc aacagctccg
     1261 agatatgacc ctcgagccca agccggcgca aacccgccgc cgccagaacg atcgcctcat
     1321 actcgccgcg ctcgagccgc tcgatccggg tcggcacatt gccgcgtaac tccagcagcc
     1381 gaagatccgg ccgtgcgcgc gcgagaaacg cccgccggcg caagctgctg gtcccgaccc
     1441 gcgcgccctc gccgagctcc gccagtcgcc tcccgtcgcg gctgacgagc gcatcgctgg
     1501 gatcctcgcg tttcagcacg gcggccagct cgataccggg ctccagtatc gtggacatgt
     1561 ccttgaggct gtgcactgcg gcatccactt cattcgacag cagcgccttg tcgatctcct
     1621 tggtgaagaa cgcgcggccg ccgatctgcc acagcggcac atcgacgcgc acgtcgcctt
     1681 cggtcttgat ccgcaccagc tccaccggtg gcggatcagg ctgtgcgcgg atcagctcct
     1741 cgagatggtg tgcctgccag agcgcgagcg ccgagccgcg ggtgccgatg cgaagcgtgc
     1801 gctcgctcat ggctcgcctt cactcaggaa acgataacca acaccccaca cggtgaggaa
     1861 atggcgcgga ttggcaggat cgcgctcgaa ccgcttgcgc aggcgcagaa tgaagttgtc
     1921 caccgtgcgc gtggagggaa acacgtcata gccccacacc cgctcgagca gatcttcgcg
     1981 cgagacgatc tcaccgggcc gctcggccag caccttgagg atcatggcct ccttctcggt
     2041 cagctcctgg gcgacaccgt tccacgagcg cgcgcggaac gcacggaaat ccacttcgtt
     2101 gccggcgaac tgcaggaccg caccggcggg cgtcgaggag gtgcttcgat accaatccca
     2161 gcgccgcagg atcgcgcgca cgcgcagcag gaactcctcg aggtgaaagg gcttggcgag
     2221 atagtcgtca ccaccggctt cgagcccgcg aacgcggtcc gcgggatctc cgcgggccgt
     2281 cagaaacaac accggcgtgc tgttgccttg ctcgcgaagc gtgcggcaca gcgtgaagcc
     2341 gtccatttcc ggaagcatga cgtccaacac gatgagactg cattgttgag cctggagcca
     2401 ttcgagcgcc ggcttgccat cgcccagcgc cacgacatcg tagccttctg cgcgcagatt
     2461 ctcggcaacc ccggcggcca gatgcgggtc gtcctccacg accagaatac gattctgcgc
     2521 cgcaggcgtg ctcatgccgg ccccgcaggg gcagtcggcc acaccagcac gaattgcgcg
     2581 ccctgaccga ctccgccgct gtgcgcactg acgcgcccgc ccgcaagctg catcagacgc
     2641 cgcacgatga agaggcccag accggtgccg tggtaattgt tgccaccgcc aggatgcagc
     2701 cgggtgaact tctcgaacag ccgcgcgcca tccgctggcc ggaagccgac gccgctgtcc
     2761 ttcaccgaca gctccacttc gccatccagc cgccgggcac cgagtgtgat cgtgccaccg
     2821 ccaacgggcg tgatggccgc gatcgcattc tcgatgagat tacggaccac gacatcgaga
     2881 gcgagaggat ccgccagaac gtacagtcct cgctcgatgt cggtcgagat ggtgatgcgc
     2941 tccttgcgtg cgcgctcatc gagctggccg acgacgcggg cgatcgcgcc actcaattcc
     3001 agcggctcgc ggcgcagatc gactcttccg cgttccaagc gcacgctctc caggatctgc
     3061 gtgaccatcg ccgccatgcg tgccagatcc gccagcatgc gatcgatcaa ggtgcgcgac
     3121 tgctccgcag agagcgagcg cattgccatc gtctcgagcg acagctgcag gctcgccagg
     3181 ggtgtcttga actggtgtga aacgagagcc agaaagctct cctgctcctg cctcaccagc
     3241 gcctcggcgc gcaaggctcg ggcaatcacg ccgatgcaca ccgcgagcgc cagcaggaag
     3301 aagccgcctt cccaggcgta ctgtcggatg cgtcgatcct tctccgtgag gagctctcga
     3361 tccacatcgg gcgagagcga ggcgtgcttc ccctcgatca ccacatcggg cagcttggcg
     3421 cgcacgctct cgggcgaggc cccggaatcc aacagagctt gcgccgcgct gagctgctga
     3481 aaatagagct cgcgtgtcgt gcgcactttc tcgatggcat aggagcgctg atcgaacagc
     3541 caccagccga cctggatgac ggccacgacc accagggcca gagcggtcag ctgaaagacc
     3601 cgggtgcctt tcatgcgatc gcggccaggg aatcgcgcag ggcctgggcg agctgcagca
     3661 catctgcgct ggtgtgcgca agcgacagga agcacgcctc ataggctgag gggggtagat
     3721 agataccgcg gtcgagcatt gcgtggaaaa gcgtgctgaa gtgcgcgccg gccttttcat
     3781 ccaacggtgc cgccgcacgc ggcgcgcccc cctcatggag cgacagccag aatagcgaac
     3841 cggatcgcac cagatggatg ggaaaaggcg cctccgccaa caccggtttg agggcgcgct
     3901 ccacttccat gcccaatgac tcgaggcgct cccaaccgga ctcccggatg aggatgtcga
     3961 gtgtggtgat gccggcggcc atggcgacgg gattgcccga aagtgtgccc gcctgatacg
     4021 tgtcccccag cggggccagt cgctgcatga tcgggcgcgg gccggcaaaa gcccccaccg
     4081 gcatgccccc gccgatcacc ttgccgaagg tcgcgaggtc cggcgtgatg cccgtctgtt
     4141 gggcgacgcc gccccgcgcg aggcgaaatc ccgagatcac ctcatcgaat atgagcaatg
     4201 ttccgtggcg ctcggtcagt gcgcgcagct tctgcaggaa cgcctttcgc tgcaacagca
     4261 gcccgtgatt ggcgggcacg ggctcgatga tcacggcagc aatgcgttgc ccttcgcgcg
     4321 aaaagagcgc ttcgaccgcg tcctcgtcat ccagaggcag gacgcgggtg caggcggtga
     4381 aggcctcggg cacacccgcc gaggaagggc gcccgaaggt ggcgagtccg gagcctgccg
     4441 ccacgagcag atgatcggca tggccgtgat agcagccgga gaacttcacg atcagatcgc
     4501 gcgcggtgaa ggcccgagcc agccggatcg cgctcatcac ggcctcggtc cccgaagaga
     4561 cgaagcgcac ctgctcgatt cccggatagg agccgactac ccgctcggcg agctcgactt
     4621 ccgccgcaca gggcgcaccg aacgtcgtgc ccagcctgct ggcctcgttg atggcggcca
     4681 cgaccgccgg atgagcatgg cccaggatca gcgcccccca ggaacccacg agatccacgt
     4741 aggtgcgccc gtcggcatcc acaatcttct ggcccgctcc ccgctggatg aacaaaggcg
     4801 tgccgccgac cgcccggaac gcgcgcacgg gtgagttgac cccaccgggg atgacccggc
     4861 aggcgcgctc gtacagatcg cgggaatgcg ggtgctcggt catggattcc gcccgttgtt
     4921 gcggcgcctc agccgcgcct cgcgaaagaa agcctctacc gtctcgctgc tcgcatgcac
     4981 ggccgccgcc cgcagccccg ccaggggcag atgcgccagg cgccgcgcca cagtcgatgc
     5041 gaaccgctgc agcagcgcac gctgattctc gtccagatcg ctgaattctg cctcgagtgc
     5101 gcgctgagcc tcttcggcgg caatctgctc gaaggcgccg cgcagatcgg cgaggcgccg
     5161 tccgatggcg cgcgcggcca gctcgctccg cagatgattc agtcggtcgt cgatggccgc
     5221 ccggaccggg gcgagcctca tgagctgcgc cagccggcgc tcctgggccg cctgaatgag
     5281 atcgtccatc ccaacacgca gcagacccag ggcgcgtgag gcttccggat cggcattggg
     5341 gggtaacgcg agatcgacca gcagcggggc tccaccggac ggcaacccgg gcggcgcctg
     5401 accagcccgc aaaccagcct ctagcccggg ccccaaaacc gggccgatac gggccttcgc
     5461 aatgttcgcc agggcggccg cgtccagcac gcgctcgccg ccccccgccg caagcaccac
     5521 agcgccgacg gcgggtggcc ggattcgaaa ctcatcaaga gtgagcgcac gcccggagaa
     5581 cagtttggcg agctcttcgg ctgcctgaag ggtgcgattc acgaccagga gggggatgcc
     5641 tgcgcgatgc agcgcggctc cgcagcgccg ggtcatgggg gacacaccca cgagcgccac
     5701 gatggacttg tccgccggag gtggtctctg cccgagatgc cgcagcacgc gctcggcggc
     5761 catgtccccg agcgatggcg cgcgagcttg cttcgcctcg atgcgatgca ctcgggtcgc
     5821 catgccaagg gcctcactca ggacccggtc cagagtcggc ccgcaggttc gggctactcg
     5881 ggcactttcc caggcacctc gaagttgggc ggcgatctcc tgctcgccgg tctgggcgga
     5941 atccaggccg caggcgagaa gaaacagatg ctcgacggcg gcctcgcccg tccaggcgcg
     6001 cagaacacgg ttggcctcgc cgggctccgg gtcgcgcccg atcagggccc ggaagaccgc
     6061 aggccgggca tccccggctg gaaccccctc gagcgtggcg tacagcacct cgacgcgatt
     6121 gcaggtgccg acatagaaca cctcggagac acccaggctg tcccgcaacg agggcaggcg
     6181 cgccgcgacc tcccccttcg ggaccgcgaa acgcgcgacc tcatcgacat tggcatgacg
     6241 gtaggacaga cccaccacgc cgatctggtg catgggccaa gcacaccacg gggagtctcc
     6301 ccgagtgtca ccgaacggtc accgcggccg gatctgggtc ttggttcggc cgcgccgggg
     6361 cttatagggg gcccttagag gcccgcccca ggggctgcag gctcagcgcc ggccgcttcg
     6421 caccaggccc atcaccagga cgcccgcgat aaagccgatg ccgagagcaa tgccgctcgc
     6481 ggcgaacggt ctcgcctcga cccggccctg cagctcatcg agctgggagc gcgcccagtc
     6541 ctgtgcctgc gcggcgcgtg cgcgtgcagc ttgtgccgcg gcgtcctttg ccgcgcgcag
     6601 atgatctttc acctgatccg tattcgccga cccaccggtc ccggatccca gatcgccatt
     6661 cttcgcattc atgctctgac cctccaactc gccgtcagct actcacaatg cgctacaacg
     6721 gtcgctgcat tcaacagttc ccgcccggcg acagcgcgag cgtggcccgt gaagctggcg
     6781 ctttgtcccc gccggcgcgc ctcctgcgct atcgtgcgac cctggcatgg aatctccttc
     6841 gagggagcgg ccaccgatga gcagacagcc tccgaaccac tccgacccgc tggccgacgc
     6901 ccagcggctc gtggcccacg atgacgagac gtatgccttc gaggccgtcg cgcctccgat
     6961 cgtgcagagc tcactcttca cgttttcgag cttcgaggag atgcgcgaca cgtttgcggg
     7021 caagagaagc cgcaacgtct actcccgcac caccaatcca acggtcaccc agttcgaggc
     7081 gaagatggcc gagctggagc agaccgatga ggccatcggc tttcccagcg gcatggccgc
     7141 catctccggc acgctcctcg ccttcgtgga gccgggagac cggatcgtct gcgtgcggca
     7201 cgtgtacccg gatgtctacc ggctgttcga gacgctgctc cggaaactca aggtctcggt
     7261 ggtctatgtg gacggggccg atcaggcggc ggttgcggaa gcgttgccgg gcgccaaggt
     7321 gttgtatctg gagagcccga ccagctggat gatgcaggcc ctggatgtca gggcgctggc
     7381 gagtctcgcc cgccgtcacg gcgcgctctc gatcttcgac aactcatggg ctacgccggt
     7441 cttccagcgc cccatccgcc acggcgtcga tctcgtcatt cattcggcct ccaagtatat
     7501 cggcggccac agtgacacgg tcgccggcgt ggtggccggt cgctcggatc tgatcggcgt
     7561 tgtcaggcgg acggtgtgtc cttatgtggg agcgaagctt gcgccgtttg aggcgtggct
     7621 gctggtgcgg ggactgcgga ctttgccggt tcgtatgaaa gcccatgagg agtcggcgct
     7681 cgtcct
//
LOCUS       DASRHN010000367         7663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_801535, whole genome shotgun sequence.
ACCESSION   DASRHN010000367 DASRHN010000000
VERSION     DASRHN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801535"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1217)
                     /locus_tag="VFB37_14815"
     CDS             complement(252..1217)
                     /locus_tag="VFB37_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZO23772.1"
                     /translation="MFETSRWKGLLAILVLAAAVCIASLLIGSAKFGPREALAALFGQ
                     GSDAARSVMMEVRLPRLLAAFGVGGSLALTGVLLQALFRNPLADPYVLGVSGGAAVGA
                     VLALMLGAAAVSVQAAAVAGAFGAVAAVYLLARGGGTPRLLLTGVVTASACGALVAVL
                     LALTDSVRLRGIVFWLAGDLSWALNPWSSALTALIATGLALAIARPLNVLSAGELRAR
                     SVGLHIEVWRTSLFVACAILTAVAVVSAGTVGFVGLITPHAIRLTFRTSDHRIVAPGA
                     VILGGTVLAAADLVARTIASPRQLPVGAIMALVGAPLFIVLLRRR"
     gene            1423..2013
                     /locus_tag="VFB37_14820"
     CDS             1423..2013
                     /locus_tag="VFB37_14820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZO23773.1"
                     /translation="MADSDVSMSDVIDSDGFRANVGIVLMRGGGDTFLGRQVGGRGWQ
                     FPQGGVRPGESLEEAAYRELNEEIGLSRSDVVLLGRTARWLRYRLPARYVRRNQRPVC
                     IGQKQRWFLMRLKLEDTRFDFERTTQPEFDQFRWVTFWEPVREVIYFKRGVYVRALTE
                     LAPLAFPEGPPQHPSWWLQTTSSTPQPNGAAPAAID"
     gene            2128..2766
                     /locus_tag="VFB37_14825"
     CDS             2128..2766
                     /locus_tag="VFB37_14825"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HZO23774.1"
                     /translation="MALYIAYEFGRFNAGYDRLAVAQQRTEYEVAIDRLQKSNRELRT
                     QLAELDTIRIGRAREQAEVARTIGDLQAQVARQTQELAFYRGIVAQGAAAVGVKVQQV
                     RITPGTKPDKFVLHVELVRSVRPDNVASGAVGVTVDGAGADGKATTIDLPALTAGRQH
                     ELPYNFRYFENLDQEVAFPAGFKPDHVAVELRSSRKDVPPATQSFPWSVDAS"
     gene            2786..3187
                     /locus_tag="VFB37_14830"
     CDS             2786..3187
                     /locus_tag="VFB37_14830"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZO23775.1"
                     /translation="MFSRDSRQPRIDTLIDKASHVYGDLVFAGGLHLDGTISGNVRAE
                     PNEGSSLSVSESGAIEGTVEVSNVMLNGTVKGDIVAREKVVLGSTAKVQGSVYYGVIE
                     MTLGAQIMGKLTRVSGDSKPESGAEQASRPL"
     gene            3248..3604
                     /gene="erpA"
                     /locus_tag="VFB37_14835"
     CDS             3248..3604
                     /gene="erpA"
                     /locus_tag="VFB37_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZO23776.1"
                     /translation="MTSTTEVREEAALLFTDAAARKVGDLIRGEGNPKLMLRVFVQGG
                     GCSGLQYGFEFDEQIQEGDTCLENLGVKLLVDPMSVQYLTGAEIDYREGLEGAQFVIR
                     NPNATTTCGCGSSFSA"
     gene            3706..3915
                     /locus_tag="VFB37_14840"
     CDS             3706..3915
                     /locus_tag="VFB37_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23777.1"
                     /translation="MPRAKDVTFALSSIGRKTPVALSADIARTPRAKDVTFALSSIGR
                     KTPVALSADIARAPRAKDVTFARFS"
     gene            3899..5395
                     /gene="ppx"
                     /locus_tag="VFB37_14845"
     CDS             3899..5395
                     /gene="ppx"
                     /locus_tag="VFB37_14845"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZO23778.1"
                     /translation="MPASPEVLAAVDLGSNSFHMVVARHSHGQLVIIDRLREMVRLAA
                     GVAENGRIDKEVAARALACLQRFGQRLRDMQADSVRVVGTNALRLAHRKQAFLERARE
                     ALGHPIEIISGMEEARLIYSGVANTMPSEPGRRLVVDIGGGSSELIIGDGLTPLELES
                     LQMGCVSLSERFFRDGKLSAKRVERARMAARLELEPVQAAFRRRGWEHCAGSSGTVRA
                     IGEAIRELDSQALWITPEGLERVIHHMIDAGHTRELRLTPITEDRRPVFPGGTVILAE
                     ILAALDIKEMRIAEGAMREGLLYDMLGRFKNEDARDRTVRAMQMRYHVDLAQAERVEA
                     SVLQFLEQTREVWHLEAPLADLALRWAARLHEIGLDVSHSGYHRHGAYLLENADMPGF
                     PREEQRLLARLVGGHRRKLALEGVEDLVPPWDRNALHVIVLLRLAVLLHRGRSPTALP
                     GIELSATARSLEVEFPQRWLRDHPLTSADLQQETEHLRSVGFRLRVFG"
     gene            complement(5399..7489)
                     /gene="ppk1"
                     /locus_tag="VFB37_14850"
     CDS             complement(5399..7489)
                     /gene="ppk1"
                     /locus_tag="VFB37_14850"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZO23779.1"
                     /translation="MDSPDLKAPQHYINRELSFLEFNQRVLAQAFDESVPLLERLRFL
                     CISSSNLDEFFEIRVAGLKQLLELGSGQFAADAMSLSDQLAAIHERALQLCAAQYRCL
                     NEVLLPQLQAEGISLQAREAWSPAAQEWLERYFEQEIEPVLSPLGLDPARPFPRIQNK
                     SLNFIVRLQGKDAFGRDSEHAIVQAPRSLPRVVPVPNDGSERTLMLLSVIVHTFVQKL
                     FAGIEVLGCYQFRVTRNSDLFVDEEEIDDLRRALEGELAHRRYGAAVRLETSSDCPED
                     MVQFLLHQFALSDVDMYRMPGPVNLNRLSAIYDLVQRPDLKYPIFTPGLPKRLLTNTD
                     MFSVIRQNDILLHHPYQSFAPVMDFVRQAAGDPQVLAIKQTLYRAGHDSPVVDALVAA
                     ANAGKDVTVIVELRARFDEEANIELSNRLQEAGAHVMYGVVGYKTHAKVTLVVRREPG
                     GIRRYCHLGTGNYHPRTARTYTDYGLLTCNEEMGQDVHELFLQLTSLTQTPKLNRLVQ
                     SPFGMHEAVISKLAREAEHARAGRPARIIAKLNALVDAQAIEALYRASCAGVKTDLIV
                     RGVCALRPGVPGVSENIKVRSIVGRFLEHSRVFYFENGGQPEMYCASADWMERNFFRR
                     VEVAFPIQREAHRQRILRDLDFCLQDNTQAWELLPDGSYRRIERGGEERAINTQAELL
                     AAYAAGPGTAFATG"
BASE COUNT         1367 a         2454 c         2526 g         1316 t
ORIGIN      
        1 ctcttcacga tccttgccgc cgcggcaaaa cgcgtgggtt ttgccgctca atcccccacg
       61 ttggccgaaa tctttgctgc aagtcgcctg cggcgctttc agcccgcgac gagcgctctt
      121 cacgatcctt gccgccgcgg caaaacgcgt gggttttgcc gctcaatccc ccacgttggc
      181 cggaatcttt gctgcaagtc gcctgcggcg ctttcagccc gcgacgagcg ctcctcacga
      241 tcctttagac gtcagcgcct tcgcagcagc acgatgaaca gcggcgcacc gaccagcgcc
      301 atgatcgcgc cgaccgggag ttggcgggga ctcgcaatgg tacgcgccac cagatcggcc
      361 gcggccagca ccgtgccgcc cagaatgacc gcgcccggag caacgatccg atggtcgctg
      421 gtgcggaagg tgagtcggat cgcgtgcggc gtgatcagac ccacgaaccc tacggtccct
      481 gcgctgacta ctgcgactgc ggtgaggatt gcgcacgcca cgaacagact cgtgcgccag
      541 acttcgatgt gcaatccaac actgcgggcg cgcaactcgc ccgcagagag gacgttgagg
      601 ggcctggcga tggccagggc gagcccggtc gcaatgagtg ccgtgagcgc gctgctccac
      661 gggttcaatg cccagctcag atcgcccgcg agccagaaca cgatgccgcg caggcggaca
      721 gagtctgtca gcgcgagcag cacggcgacg agggcgccac aggcgctggc ggttaccaca
      781 ccggtgagta gcagacgagg cgttccgccg ccgcgagcga ggaggtagac cgccgcgacg
      841 gcaccgaaag ctccggccac cgctgcggcc tgtacgctca ctgcggccgc gccgagcatc
      901 agggccaaca ctgctccgac ggctgcgccg cccgagactc cgagcacata aggatcggcg
      961 agcgggttgc gaaacaaggc ctgaagcagc accccggtca gtgccagcga tccgcccacg
     1021 ccgaaagctg cgagcaggcg cggcaggcgc acctccatca tgacgctgcg cgcggcatcg
     1081 ctgccttgcc caaacaaggc cgccagcgcc tcacgcggcc cgaacttggc gctgccgatc
     1141 agaagcgatg caatgcagac tgcagcagcg agcacgagga tcgccagcag ccccttccat
     1201 cgggacgttt cgaacattcc gggcctcgcg gccgttgttg cgccagatcc tggcatgcct
     1261 cagacccccg agcagcgtgt cggacttgcg gcgccctttt ggctcggcca gcggaaatcg
     1321 ccactcgcgc gggagcgcgg cagccgtctt ggtcccagat aaccgagtgc gtcacattca
     1381 caaagcgcca cgccgtatca tcccgcatcc tgagggcgcc ttgtggcaga ctcggacgtc
     1441 tccatgagtg atgtcatcga ctccgatggt tttcgcgcca acgtcggcat cgttctgatg
     1501 cggggcggcg gggacacctt tctcggccgg caggtcggcg ggcgcggctg gcaatttccg
     1561 cagggcggtg tgcggcccgg cgaatcgctc gaagaggctg cctaccgcga gctgaacgag
     1621 gaaatcggcc tctcgcgctc ggatgtcgtg ctgctgggcc gcacggctcg atggcttcgc
     1681 taccggctcc cggcccggta tgtgcgccgc aaccagcggc ccgtttgcat cggccagaag
     1741 cagcgctggt tcctcatgcg actgaagctc gaggacaccc ggttcgactt cgagcgcacc
     1801 acgcagcccg agtttgacca gttcagatgg gtcactttct gggaaccggt acgggaagtc
     1861 atctatttca agcggggcgt gtacgtgcgc gccttgaccg agctggcgcc gctggccttt
     1921 ccggagggtc cccctcaaca cccctcgtgg tggctgcaga ccacctcgag cacgcctcag
     1981 ccgaacggcg cggcgcccgc agcgatcgac tgagagggcg ccgaaggatc catgcccagc
     2041 accgactcaa gacccacact cgtcgtacgc gcacattcgc ccgcgcggcg gctcatcatt
     2101 gttggcacgc tgctgctaat ctctgtcgtg gccctgtaca tcgcgtatga gttcggccgc
     2161 ttcaatgccg gctacgatcg tctggcagtt gcccagcagc gaaccgagta cgaagtcgcg
     2221 atcgaccggc tgcagaaatc caatcgcgag ctgcggacgc agctcgcgga gctcgatacc
     2281 atccggattg gccgcgcgcg cgagcaggct gaagtcgctc gcacgatcgg cgaccttcag
     2341 gcgcaggtcg cgcgccagac ccaggagctg gcgttctatc gggggatcgt ggcgcagggc
     2401 gctgcggcgg tgggcgtcaa ggttcagcag gttcgcatca ccccgggcac gaagccggac
     2461 aaattcgtcc tgcacgtcga gctggtgcgc tccgtccgcc ccgacaacgt cgcttccgga
     2521 gccgtgggcg tcacggtcga tggggcgggt gccgacggga aggccaccac catcgacctg
     2581 cctgctctca cggcgggcag gcagcatgag ttgccgtaca attttcgcta tttcgagaac
     2641 ctcgatcagg aagtcgcgtt tccggccggg ttcaagccgg atcacgtcgc cgtcgagctc
     2701 cggtccagcc gcaaggacgt gccgccagcg acacagagct ttccctggtc ggtggatgcg
     2761 tcgtagtcaa agcgagtacc caacgatgtt cagtcgtgat tcccgccagc ctcgcatcga
     2821 tacgctgatc gacaaggcgt cacacgttta cggcgatctc gtgttcgcgg gagggttgca
     2881 tctggacggc accatctcgg ggaatgtgcg ggccgagcct aacgagggct cgagcttgtc
     2941 cgttagcgag agcggggcga tcgaaggcac tgtcgaggtc tcgaacgtta tgttgaacgg
     3001 gacggtcaag ggcgacatcg tggcgcgcga aaaggtggtg ctgggctcca ctgcgaaggt
     3061 gcaaggcagt gtttattatg gggtcattga aatgacgctg ggagcgcaga ttatggggaa
     3121 gctgacccgc gtttccgggg acagcaagcc cgagtcgggg gcggagcagg ccagcagacc
     3181 cctttgacca ccccctggcg ggtccgcggc tgctagactg gtcctgcgac ccgaagagga
     3241 gaggaagttg acgagcacca ccgaagtaag ggaagaggca gcactcctgt tcacggatgc
     3301 ggcagcccgc aaggtgggcg atctcatccg gggcgagggt aaccccaagc tcatgctgcg
     3361 cgttttcgtc cagggcgggg gctgctcggg cctgcagtac gggttcgagt tcgatgagca
     3421 gatccaggaa ggcgatacct gcctggagaa cctcggggtc aagctgctcg tagaccccat
     3481 gagcgttcag tacctcaccg gagcggaaat cgactaccgc gagggcctcg agggcgcgca
     3541 gttcgtcatc cgcaacccca acgcgacgac cacctgcggg tgcggctcct cattctccgc
     3601 gtaaaacgcg cggcccgcgg cgtagaccgc cggcaaaggg cctagctgtt gcccgcacat
     3661 tcattggccg aaagacgccg gtggcgcttt cggcagacat cgcccgtgcc ccgggcaaag
     3721 gacgtgacct ttgccctgtc atccattggc cgaaagacgc cggtggcgct ttcggcagac
     3781 atcgcccgta ccccgcgggc aaaggacgtg acctttgccc tgtcatccat cggccgaaag
     3841 acgccggtgg cgctttcggc agacatcgcc cgtgccccgc gggcaaagga cgtgaccttt
     3901 gcccgcttct cctgaagtcc tggcagccgt cgacctcggg tccaacagct tccacatggt
     3961 cgtggcgcgg cactcgcacg gacaactcgt catcatcgat cggctgcgcg agatggtgcg
     4021 gctggcggcc ggggtggcgg aaaatggccg catcgacaag gaagtggccg cgcgagccct
     4081 cgcatgtctg cagcggttcg ggcagcgtct gcgggacatg caggccgaca gtgttcgcgt
     4141 cgtcggcacc aacgcacttc gactcgcaca tcgcaaacag gcgttcctgg agcgcgcgcg
     4201 cgaggctctc gggcatccca tcgagatcat ctccggtatg gaggaagcgc gcctgatcta
     4261 ttcgggtgtc gccaacacga tgcccagcga gcccggcagg cggctggtcg tggacatcgg
     4321 tggggggagc tcggagttga tcattggcga tggcctcact ccgctcgagc tggaaagtct
     4381 gcagatgggc tgtgtctcgc tcagcgagcg gttctttcgt gacggcaagc tttctgcgaa
     4441 gcgggtcgag cgcgcacgca tggcggctcg cctggagctc gagcccgtac aggcggcctt
     4501 tcgccgccgg ggctgggagc attgcgccgg gagctcgggc accgtccgcg caatcggcga
     4561 agcgatccgc gagctcgatt ctcaggcgct gtggatcaca ccggaaggcc tggagcgggt
     4621 catccatcac atgatcgacg cggggcacac gcgtgagctg cggctgacgc ccatcactga
     4681 agatcgcaga ccggtgtttc ccggtggaac cgtcattctc gcggagatcc tcgcagccct
     4741 tgacatcaag gagatgcgca tcgcggaagg agcgatgcgc gaaggcctgc tctacgacat
     4801 gttggggcgt ttcaagaacg aggacgccag agatcgcacg gtgcgagcga tgcagatgcg
     4861 ctatcacgtg gatctcgctc aggccgagcg tgtggaggca agtgtcctgc aattcctcga
     4921 gcaaacccgc gaggtgtggc atctagaggc tccccttgcg gacctggcac tgcgctgggc
     4981 cgccaggcta catgagatcg gcctggatgt ttcccacagt ggctatcacc gtcatggcgc
     5041 ctacctgctg gaaaacgcag acatgccggg gtttccccgc gaggagcagc ggctgctcgc
     5101 gcggctggtc ggcggtcacc ggcgcaagct cgcactcgaa ggtgtagagg acctggtccc
     5161 gccctgggac cgaaacgcgt tgcacgtgat cgtgctgctg cgtctggccg ttttgctgca
     5221 tcggggacga agtccgacag cgctgccagg catcgagctc agcgccacgg cccgctcgct
     5281 ggaagtggaa tttccgcagc gctggctgag ggatcacccg ctcacatcgg ccgacctgca
     5341 gcaggagacc gagcacctgc gctcggtggg ctttcgcttg cgcgtattcg gttgagggtc
     5401 agcctgtagc gaaggccgtg ccgggacctg ccgcgtaggc ggccagcagc tcggcttgag
     5461 tgttgatcgc acgctcttcg ccgccgcgct cgatccgacg atagcttccg tcggggagca
     5521 gttcccaggc ctgagtgttg tcctgcagac agaaatccag atcgcgcagg atccgctgtc
     5581 ggtgtgcttc acgctggatc gggaaggcca cttccacgcg gcggaagaaa ttgcgctcca
     5641 tccagtccgc gctcgcgcag tacatctccg gctgaccgcc attttcgaaa tagaacaccc
     5701 gcgagtgctc gagaaagcgg ccgacgatgg agcgtacctt gatgttctcg gacacgccgg
     5761 ggacgccggg acgcagtgcg caaacgccgc gcacgatcag atcggtcttc acgccggcgc
     5821 atgaggcccg gtacagcgcc tcgatggcct gggcgtcgac cagggcattg agcttggcga
     5881 ttatgcgtgc gggtcgtccg gcacgcgcgt gctctgcttc acgtgcgagc ttggagatca
     5941 ccgcctcatg catgccgaag ggcgactgca ccagccgatt caacttcggc gtctgggtga
     6001 ggctggtgag ctgcaggaac agctcgtgga catcctgacc catctcctcg ttgcaggtga
     6061 gcagtccata gtccgtgtag gtccttgcgg tgcgagggtg atagttgccc gtgcccaggt
     6121 ggcagtagcg gcggatccca ccgggctcgc gtcgcacgac cagcgtcacc ttggcgtgcg
     6181 tcttgtagcc gactacgccg tacatgacgt gggcgcccgc ctcctgcagg cggttggaca
     6241 gctcgatgtt ggcttcctca tcgaagcgcg cgcgcagctc gactatgacg gtgacgtcct
     6301 tgccggcgtt ggcggcggcg accagcgcat cgaccaccgg tgagtcgtgc cccgcccgat
     6361 agagtgtctg cttgatggcc agaacctggg ggtcgccagc cgcctggcgc acgaagtcca
     6421 tgaccggggc aaagctctga taggggtgat gcagcagaat gtcgttctgc ctgatcaccg
     6481 agaacatgtc ggtgttggtg agcagccgct tgggcaggcc cggcgtgaag atcggatact
     6541 tcaggtccgg acgctgcacc aggtcataaa tggccgatag ccggttcagg tttacggggc
     6601 ccggcatgcg atacatgtcc acgtccgaga gggcgaactg gtgcagcagg aactgcacca
     6661 tgtcttccgg acagtcgctc gaagtctcca ggcgcacggc tgccccgtag cggcgatgcg
     6721 caagctcgcc ctccagggca cggcgcagat cgtcgatttc ctcctcgtcc acgaacagat
     6781 cgctgttgcg ggtgacgcgg aactggtagc agccgagcac ctcgatgccc gcgaacagct
     6841 tctgcacgaa ggtgtgcacg atgaccgaaa gcagcatgag tgtgcgctcg gacccatcgt
     6901 tcggcaccgg gaccacgcga ggcagcgatc ttggcgcctg cacgatggcg tgctcgctgt
     6961 cgcgaccaaa ggcatccttg ccctgcagtc gcacgatgaa attgaggctc ttgttctgga
     7021 tgcggggaaa cggtcgcgcc ggatcgagcc cgaggggact gagtaccggc tcgatctcct
     7081 gctcgaaata gcgctccagc cattcctgcg ccgcggggct ccaggcttcg cgtgcctgca
     7141 acgagatgcc ttcggcctgc agctgtggca gcagcacctc gttcaggcag cggtactgtg
     7201 ccgcacacag ctgcagcgcc cgctcgtgaa tcgccgcgag ctgatcggag agcgacatcg
     7261 catctgcggc gaactgcccc gagccgagct cgagcagctg cttcaatcct gcgacacgaa
     7321 tctcgaagaa ctcatccaga ttgctggagg agatgcagag gaatcgcagc cgctcgagca
     7381 gcggcaccga ttcatcgaac gcctgcgcaa gcacgcgctg gttgaactcg aggaacgaca
     7441 gctcgcgatt gatgtagtgc tggggtgcct tgagatcggg cgagtccatc gatggattac
     7501 ctcggcgcgc gcttcagctc aacgagcagc tgctcgagtg cgcaactgag tccggtttcg
     7561 atgacagctt gatgacactt cagcgcgcca ccagcgccgt gccgagcgga gtctccacgg
     7621 tcgccagcag cggctcggac ttgctcagga agtcctcgcg cca
//
LOCUS       DASRHN010000368         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_298657, whole genome shotgun sequence.
ACCESSION   DASRHN010000368 DASRHN010000000
VERSION     DASRHN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298657"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /gene="aepX"
                     /locus_tag="VFB37_14855"
     CDS             complement(<1..1282)
                     /gene="aepX"
                     /locus_tag="VFB37_14855"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HZO23780.1"
                     /translation="MDSANARSAPGAGASEPDKAPSRSAKLRRLLLSERLEFLMEAHN
                     GLSARIVREAGFPGIWASGLSISAQFGVRDNNEASWTQIVDMLEFMADASDLPILLDG
                     DTGYGNFNNLRRLVRKLEQRGIAGVCIEDKQFPKTNSFLQSERQPLADMAEFAGKIAA
                     GKDTQSDANFSIVARVEALIAGWGMEEALRRAEAYRRAGADAILIHSKMSKPDEIVSF
                     AREWARRAPLVIVPTRYYSTPTEVFREAGISVVIWANHMVRSATAAMQNVAREIHSSE
                     TLVNVEDRIAPVNEIFRLQNVEEYSAAERLYLSQSRPNRAAIVLAATRGRGLEAVTME
                     RPKVMLPIAGKPLLRWLVDSFKKESVNDITVVGGYRADAIDTSGVRLVVNERYEDTGE
                     LTSLACAVDGLDCDTVIAYGDLLFRSYVLRDLLES"
     gene            complement(1285..2046)
                     /locus_tag="VFB37_14860"
     CDS             complement(1285..2046)
                     /locus_tag="VFB37_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZO23781.1"
                     /translation="MRAILLAAGRGLRLQQPEDAQSPKCLLQFGGRTLLERHLRLLRS
                     AGVEDIVLALGFRHELVTTELDRLRWQPRPRIVLNARFELGSVLTVHTVADEMLRGGE
                     VLLMDADVLYDRRMMIALTAGHTPVNRLLIDRNYEAGEEPVKLCIRDGVPVELRKQVP
                     ADLRYDTIGESVGFFRFDESGARRLANIVADYAANERGNLPHEEAVRDLLREGSQVFE
                     VADVTGVPWIEIDYFADVTRAGSEVLPQLEPLPGE"
     gene            complement(2060..3061)
                     /locus_tag="VFB37_14865"
     CDS             complement(2060..3061)
                     /locus_tag="VFB37_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZO23782.1"
                     /translation="MSRAAIWLLSAGVLLFAGVVLSQGITAVFSTLALAGWGLIVVAL
                     WHLVPLVLDAGSIRVLFADTVRGGSLRDSVLARWAGESANSFLPAGPISGPVVMARHL
                     AQRGMPMQEAAASITVSTTLQTLAQVVFGLAGFTLLLGQASGVLRQSVLGPLLIASSV
                     VALCLAGFYLMQRRGLFGKLMRATSRLSARRDWSELLSQAAAIDQTIREVYARRARAI
                     ASFALSLIGWVVGTVEVYLILRLLGSPVDWSKALLLESLGQAIRSAGFAVPGSLGVQE
                     GGYLLLAPLAGLQPNTAVALSLAKRAREILLGLPGLLYVHLAERRWRRREACAAAVD"
     gene            complement(3058..>3594)
                     /locus_tag="VFB37_14870"
     CDS             complement(3058..>3594)
                     /locus_tag="VFB37_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23783.1"
                     /translation="QGGSVSRHTIDAQAGLIAELYRSAALMEWLARLAGEPLQLSPPE
                     DPHAYALYYYTRAGDHIGWHYDTSYYAGRRYTLLLGVIDESSCRLDYELYTRERGRTS
                     MRGSVRIPPGGLVFFDGDRLRHRISPLGEGERRVSLTFEYVTDPAMAWGWRWISNLKD
                     ALGYFGFRQVFRRARRRT"
BASE COUNT          672 a         1242 c         1110 g          570 t
ORIGIN      
        1 gactttcgag cagatcgcgc aacacatagc tgcgaaagag cagatctccg tacgcgatca
       61 cagtgtcgca atccaacccg tccaccgcac aagcgagcga ggtgagctcc ccggtgtcct
      121 cgtaccgctc attgaccacg aggcggactc ccgaagtgtc gatcgcatcg gcgcgatagc
      181 cgccgacgac ggtgatgtcg ttgacgctct ccttcttgaa gctgtccacc agccagcgca
      241 gcagcggctt gccggcgatc ggcagcatga ccttcggtcg ctccatcgtg accgcctcca
      301 gcccgcggcc gcgggtggct gccagcacga tggccgcacg gttcggacgc gactgcgaga
      361 gatagaggcg ctccgcagcg gagtattcct ccacgttctg caggcggaat atttcgttga
      421 ccggggcaat acggtcctcg acgttcacca gagtctcgga agagtggatc tcgcgcgcga
      481 cattctgcat ggcggcggtg gccgagcgca ccatgtgatt ggcccagatc accacactga
      541 tgccggcctc gcgaaacacc tcggtcggag tgctgtaata gcgcgtcggc acgatcacca
      601 gcggcgcgcg tcgtgcccac tcacgcgcaa aactcacgat ctcatcgggc ttcgacatct
      661 tgctgtgaat caggatcgca tccgcgccgg cgcgccggta agcctcggcg cgccgcagag
      721 cctcctccat accccagccg gcgatcaacg cctcgacacg cgcgacgatc gagaagtttg
      781 catcgctttg cgtatccttg ccggctgcga tcttgccggc aaattccgcc atgtcggcca
      841 gcggctggcg ctcgctctgc aggaagctgt tggtcttggg aaactgcttg tcttcgatgc
      901 acacgcccgc gatgccccgc tgctcgagct tgcggaccag ccggcgcaga ttgttgaagt
      961 ttccgtaacc cgtatcgcca tcgagcagga tgggcagatc gctcgcatcg gccatgaact
     1021 cgagcatgtc cacgatctgt gtccagctcg cctcattgtt gtcgcgtact ccgaactgcg
     1081 cggagatcga cagccccgaa gcccagatgc ccgggaagcc ggcctcgcgc acgattcgcg
     1141 ccgacagccc gttatgcgct tccatcagga actccagccg ctcgctgagc agcaggcggc
     1201 gcagcttggc gctgcggctc ggcgctttgt ccggttcgct cgcgccggcg ccgggcgcag
     1261 acctcgcatt tgcagagtcc atcgtcactc ccctggcaaa ggctcgagct gcggcaacac
     1321 ctcgctgccc gcccgggtta catcggcgaa atagtcaatt tcgatccacg gcactccggt
     1381 cacatccgcc acctcgaata cctgactacc ctcgcgcagc agatcgcgca cggcttcctc
     1441 gtgcggcaga ttgccgcgct cattggcagc atagtccgcg acgatgttgg ccaggcgccg
     1501 cgcacccgat tcatcaaaac ggaagaagcc gacggactcg ccgattgtgt cgtaccgcag
     1561 gtcggccggc acctgcttgc gcagctcgac gggtacacca tcgcggatgc aaagcttcac
     1621 gggctcctcg ccggcttcgt aattccggtc gatcaacagg cggttcacgg gggtatggcc
     1681 cgcggtcagt gcgatcatca tgcggcggtc gtacagcaca tctgcgtcca tcagcaggac
     1741 ctcgcccccg cggagcatct cgtcggcaac agtatgaacc gtcagtacgc tgccgagctc
     1801 gaaccgggca ttcaggacga tgcgcgggcg cggttgccag cgcaggcgat ccagctcggt
     1861 ggtgacgagc tcgtggcgaa atccgagggc cagcacgata tcctcgacgc ccgcacttcg
     1921 aagcagccgc aagtgtcgtt ccagcagtgt ccgtccgccg aactgcagca ggcacttggg
     1981 actctgcgca tcttccggtt gctgcaggcg caggccgcgg cctgctgcta gaaggatggc
     2041 tcgcatcggg ttcctcgggt taatccaccg cggcagcgca agcttcacgg cgccgccagc
     2101 gccgctcggc caggtgcacg tagagcagcc cggggagccc cagcaggatc tcgcgggcgc
     2161 gcttcgccag cgagagtgcc acggcggtat tgggctgcaa gcccgccagg ggggccagca
     2221 gcagatagcc gccttcctgc acacccagcg agccaggaac ggcaaagccc gcactgcgga
     2281 tcgcctgccc caagctctcc agcagcagcg ccttgctcca gtctaccggt gagccaagca
     2341 ggcgcaggat cagatagacc tccacggtgc ccaccaccca gccgatgagg ctcagggcga
     2401 agctggcgat cgcgcgagca cgccgggcat agacctcccg gatcgtctga tcgatcgcgg
     2461 ccgcctggct caatagctcg gaccagtcgc gccgcgctga aaggcgcgag gtcgcgcgca
     2521 tcagcttgcc gaatagcccg cgccgctgca tgagatagaa gcctgccagg cacagcgcga
     2581 ctacgctgct cgcgatcaga agcggcccga gcaccgactg ccgcagcacg ccgctggcct
     2641 gcccgagcag cagcgtgaaa cctgccagac cgaaaacaac ctgggcgagg gtctgcagcg
     2701 tcgtgctgac cgtgatactg gccgccgctt cctgcatcgg catgccgcgc tgcgccagat
     2761 ggcgggccat gacgaccggt ccgctgatcg ggccagccgg cagaaaactg ttggcggact
     2821 cgcccgccca gcgggccagc acggaatcac gcagtgagcc accgcgcacc gtatcagcga
     2881 acagaacgcg gatcgatccg gcatcgagca ccagtggcac cagatgccac agcgcgacca
     2941 caatgagacc ccatcccgcc agcgccagcg tcgaaaacac cgccgtgatc ccctgtgaaa
     3001 ggaccactcc ggcgaacagc agcacgcccg ccgagagcag ccagatagcc gcccggctca
     3061 tgttcttcgg cgggcgcgcc ggaatacctg gcgaaagccg aagtacccga gcgcatcctt
     3121 gaggttggaa atccatcgcc aaccccacgc catggccggg tcggtgacat actcgaaagt
     3181 cagcgagaca cgcctctcgc cttcgcccag tggcgagatc cgatggcgca gtcggtcgcc
     3241 atcgaagaag accagccctc ccggcggtat gcggaccgag ccccgcatgc tggtccggcc
     3301 ccgctcgcgc gtgtacagct cgtaatcgag ccggcaggat gattcgtcga tcacccccaa
     3361 caacagcgtg tagcgacgtc cagcgtagta ggaggtgtcg tagtgccagc cgatgtgatc
     3421 tccggcacgc gtgtagtagt aaagcgcgta cgcgtgcgga tcctccggcg gcgaaagctg
     3481 cagaggctca ccggcgaggc gagccagcca ctccatcagg gcggccgagc ggtacagttc
     3541 ggcaatgagg cccgcctgcg cgtcgatggt atgccggctg acgctgccgc cctg
//
LOCUS       DASRHN010000369         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_802485, whole genome shotgun sequence.
ACCESSION   DASRHN010000369 DASRHN010000000
VERSION     DASRHN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802485"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1309)
                     /locus_tag="VFB37_14875"
     CDS             complement(41..1309)
                     /locus_tag="VFB37_14875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO23784.1"
                     /translation="MITVPRLDLDVFPDSPYAAELQQGRPKLRFQPALEADYLRSMLL
                     HSRALVRVTCVFAAVLTILRGIEQSVSGDWSRIFVVDIIAVAVISLVLAWVAWSSHFE
                     RLYPLCAPILLPLRNTIVAAQISWAAANGHVEMLMVLPIMVIGPLYFLGLRFRAAIAC
                     VVVTGAAYVWAATYFELPRLIAMRSFAFLLVGVLACLIAALHLELSARRSFLEGRLIA
                     ELAQRDALTGTKNRRALDEHLTYLWQRAIQQEAPLAVLLIDIDHFKPYNDLYGHQAGD
                     QTLRRVAQAVQRFVRGPLDMLARYGGEEFAVILYDVDGNRACDIADLIRGAVAEMAIK
                     HDGAPIAGHVTISVGVAAVEPTPDRTPRGALQLADQALYEAKVGGRNRVELMDRTAHS
                     RMETGIFAVSPANSAAIAVNLTSSRRKIRR"
     gene            1458..1892
                     /locus_tag="VFB37_14880"
     CDS             1458..1892
                     /locus_tag="VFB37_14880"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZO23785.1"
                     /translation="MAIGVAVLAAGTVILRLYDYWQETSFEEAAATGAPGQPGHLVAA
                     RPGPGFPSLSDYYPPESKRQHEEGSVIVRVCVDTDGELTGDPVIAQASGVARLDQAAL
                     RVARAASGRYQPATVDGKPVKSCTKFRIRFQLKKGPPDPPKS"
     gene            1905..>2390
                     /locus_tag="VFB37_14885"
     CDS             1905..>2390
                     /locus_tag="VFB37_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO23786.1"
                     /translation="MIFEHLTAGGCQSYLIGCADSCTGVLIDPEISLIDRYLSLAARD
                     GLRIHYMIDTHTHADHFTATRQLAKELDVPVAMHRESPAPFVDLRLDDGEMLAVGKLR
                     LQVLHTPGHTRDSMCLRVEDRLFTGDTLLMGATGRTDLPSGDPEALFDSLFNRLCRLD
                     PA"
BASE COUNT          441 a          741 c          799 g          409 t
ORIGIN      
        1 atgcaggcgg gaccgccggc cgctcacgaa gcgacgatct tcaacgtcga atctttcttc
       61 ggctggacgt tagattcaca gcgattgctg cggagtttgc ggggctgacg gcgaagattc
      121 ccgtctccat gcggctgtgc gcggtgcgat ccatgagctc gacccggttg cgtccaccga
      181 ccttcgcttc gtacagcgcc tgatcggcca gctgcagcgc ccctcgtgga gtgcgatcag
      241 gggtcggctc aacggctgcc acgcctacac tgatcgtcac gtgccccgcg atcggtgcgc
      301 cgtcgtgctt gatggccatc tctgcgacgg ctccgcggat cagatcggcg atgtcgcagg
      361 cccggttgcc gtcgacatcg taaaggatga ccgcgaattc ctcgccgccg tagcgggcga
      421 gcatgtccag agggccgcgt acgaagcgct ggacggcctg ggcgactcgc cgcagggtct
      481 gatcgcccgc ctggtgccca tagagatcgt tgtagggttt gaagtgatcg atgtcgatca
      541 gcagcaccgc gagcggcgcc tcctgctgga tcgcccgctg ccacagatag gtgaggtgct
      601 cgtccagggc acgccggttt ttcgtgccgg tcagcgcatc gcgttgggcg agctccgcga
      661 tgagccgtcc ttccagaaag gagcggcgcg cgctgagctc gaggtgcagg gcggcgatga
      721 gacaggcgag gacgcccacg agcaggaagg caaatgagcg catcgcgatc aggcgcggca
      781 attcgaagta ggtggcagcc cacacgtatg ccgcgccggt cacgacaacg cacgctatcg
      841 ccgccctgaa gcgcagtccc aggaaataga gcggaccgat caccatgatc ggcagcacca
      901 tcagcatctc gacgtggccg ttcgccgcgg cccacgaaat ctgcgccgcc acgatggtat
      961 ttctcagtgg caggaggatg ggcgcgcaga gcggatagag gcgctcgaaa tgcgaactcc
     1021 aggccaccca cgccagaacc agcgagatca cggcgacagc gatgatgtcg actacgaaga
     1081 tacggctcca gtcaccggac acagactgct cgatgccgcg caggatcgtc agaacagcgg
     1141 caaatacgca cgtcacgcgc acgagcgcgc ggctatggag gagcatcgag cgcaggtaat
     1201 cggcttccag agctggctga aaccgcagct tcgggcgccc ctgttgcagt tccgccgcat
     1261 aaggggagtc tggaaagaca tcgaggtcca ggcgaggtac cgttatcacc gcgaaatgat
     1321 aagtcgcatg cgcggatggc cggtgtgatg cgcttggcga ggcttggttg ttgcgttccg
     1381 tccgactaaa gtcagggaag gcggggtaag cggttttgca gggggagccg caggtggcca
     1441 tcggcaaagg agcggtcgtg gcgatcgggg tcgccgtcct tgcggcgggg accgtgatcc
     1501 tgcggcttta cgactattgg caggaaacaa gcttcgagga ggccgccgcc accggggccc
     1561 ccggccagcc cggtcacctc gtcgcggcgc gccccggacc gggtttcccg tccctgagcg
     1621 actactatcc gcccgagtcg aagcggcagc acgaagaggg gtctgtcata gtgcgagtct
     1681 gcgtcgatac cgacggggag ctcaccggcg atccggtgat tgcccaggcg tcgggcgttg
     1741 caaggctcga tcaagcagcc ctgcgggtgg ccagagcggc atcgggccgc tatcaacccg
     1801 cgaccgtgga cgggaagccc gtgaagtcct gtacgaagtt ccggatccgg ttccagctga
     1861 aaaaaggccc gccggatccg ccgaaatcct gaggcgacct gagcatgatt ttcgagcatc
     1921 tcaccgctgg cggctgccag tcgtatctca tcgggtgcgc cgacagctgc acgggcgtgc
     1981 tcatcgatcc ggagatcagt ctaatcgacc ggtacctgtc gctggccgcg cgcgatggcc
     2041 tgcgcattca ctacatgatc gatacgcata cgcatgccga tcacttcacg gccacacgcc
     2101 agttggcgaa ggaactggat gtgcccgtgg cgatgcatcg tgagagtccc gcgccgttcg
     2161 tggatttgcg cctggatgac ggggagatgc tggccgtcgg caagctgcgc ctgcaggtac
     2221 tgcatactcc ggggcacaca cgcgactcga tgtgcctgcg cgttgaggat cgattgttca
     2281 ccggcgatac ccttctcatg ggagcgaccg ggcgcaccga tctgcccagc ggcgatccgg
     2341 aggcactgtt cgacagcctg ttcaatcgcc tctgtcgcct cgacccggcg
//
LOCUS       DASRHN010000370         8342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1612420, whole genome shotgun sequence.
ACCESSION   DASRHN010000370 DASRHN010000000
VERSION     DASRHN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612420"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFB37_14890"
     CDS             complement(<1..458)
                     /locus_tag="VFB37_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO23787.1"
                     /translation="MRLEYDPALPITAHRAEILAALVQHRVIVVCGATGSGKSTQLPK
                     LCLEAGRASAGLIGHTQPRRIAARALAGRLAQELGTPLVSGLVGYQVRFTDRTGPATR
                     IKLMTDGILLRELEGDRRLRRYDTLIIDEAHERSLNIDLLLGVLKQLTAER"
     gene            complement(464..1516)
                     /gene="hppD"
                     /locus_tag="VFB37_14895"
     CDS             complement(464..1516)
                     /gene="hppD"
                     /locus_tag="VFB37_14895"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HZO23788.1"
                     /translation="MTETYANPMGTDGFEFVEYTAPDPQLLRTLFERLGFPVAARHRS
                     KNVTLHRQGDVNFIINAEPDSFAQGFAREHGPSACAMAFRVKDAAFAYRRALELGAKP
                     GPQTAGPMELNIPSIEGIGGSLIYLVDLYGERSIYDVDFRPEPSAALNEGAGLVCIDH
                     LTHNVMRGRMDVWAGFYSKLFNFREIRYFDIEGQHTGLFSRAMTSPCGKIRIPINESQ
                     DDKSQIEEYLREYHGEGIQHIALGTKDIYRTVDALRRQGVSFQNTPDTYYEAVDERVP
                     GHRESLRELQQRGILIDGNPSKGEGVLLQIFTQNVIGPIFFEIIQRKGNEGFGEGNFR
                     ALFESIELDQLRRGVL"
     gene            1715..6586
                     /locus_tag="VFB37_14900"
     CDS             1715..6586
                     /locus_tag="VFB37_14900"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HZO23789.1"
                     /translation="MSTQAPVSSEDSRLLHSIPPSRVALIEGIALAAARRNRGALQQR
                     YVRAYFRGVGEEDLAERAPSVLARAALEHLELGSQRRRPGQPLVRVFNPDPQRDGFES
                     PHTVVMLVTDDMPFLVDSVGIVFSRADIAVHLIIHPVLEIQRDGRGRLTGLGANGSQG
                     AHAESWQFYEIDRQTDPAQLEKLKRRIETTLADVRTVVEDWRPMRAQTKTLITALESN
                     PPPLSADEVSEARLLLDWMEGRHFVYLGYRYYRLERGANEDRLIPDPRSGLGILRTPA
                     SGSRPAPIVLRGEMRARARERELLILTKANSTATVHRGEFLDYVGVKNFDERGRVCGE
                     YRFLGLWTSTAYHGSPRDIPVLRRKVQQVIEYFGLDPQSHDGKAVLNVLETYPRDELF
                     QAAVEDLIRIARGVVNLYERRTVRLLVRRDPYHRFYSCLIYVPRDRYNTEVRQRIEQI
                     VLEGFSGRSVETQVQISGSNHARVHVVVHTDPESKPKVDLAAVEHRIAEAALTWTDRL
                     RDVLIENKGEAAGLALASRYRRSFAMAYEEDVQPRDALDDLADLEALREQSAAAASDA
                     RGGTLRLSLHRPAQQKPERVHLKIVKLGDPVPISDLLPMLENFGLRVISERPYELAWP
                     EGGAAWIQDFELEHRDRLLIDIARVENPFKDAFLAAWRGEVENDGFNRLLIVAGLTAR
                     EIVVLRAYCRYLLQTGVPFSQAYMERTLAANAAIARNLVRLFERRFDADSSAPARGAV
                     READKLVAQLRTDLDSVTSLDEDRILRAYLNVVEATLRTNFYQPDKPYLAVKLDPSKI
                     PDLPLPRPKFEVFVYSPRVEAVHLRMGYVARGGIRWSDRREDFRTEILGLMKAQNVKN
                     TLIVPVGAKGGFVCKKLPTGSREEVQAEVIACYQYLIRGLLDLTDNIVGGRIVPPARV
                     VRRDPDDAYLVVAADKGTATFSDIANAISLEYGYWLGDACASGGSAGYDHKKMAITAR
                     GAWECVKRHFREIGTDIQKKDFTVIGIGDMSGDVFGNGMLLSRHIRLQAAFDHRHIFL
                     DPAPDATTSFAERQRMFDLPRSSWDDYDRKKISRGGGVFPRSAKSIALAPEAAAMLGL
                     APGAAAPNEVIRGILRMPADLLWNGGIGTYVKSSEESNAEVGDRTNDALRIDGRELRV
                     KVVGEGGNLGFSQRGRVEYALAGGRLNTDFIDNSAGVNTSDVEVNIKILLNPLMQAGK
                     LTRGERNRLLARMTNEIATLVLRNNYLQSQAISTLELQAAARLAEYQHLIRTLERSGE
                     LNRALEFLPADDELADRRKRGAGLTRPELSILLAYSKIWLTHHLLDSDVPEDPYLSSE
                     LVRYFPAPVQERFPRAIAQHRLRREIITTATTNSLVNRMGPAFVPRAQEDTGAEPAQI
                     ARAYTAAREIFDMRETWAEIEALDTKVPAKLQYSMAFQTSRLLRHVTYWLLAHRKREL
                     HVDGAVAEFRRGVRQLQAEVTHVLAGSDRERFEKLRREHMEAGVPAKLAERIASLAAN
                     NAALDIVEIATRHEVSVAQAACAYFEVGARVGLDWLREQIEQLSVEGPWQAIARTGLR
                     GAAMRIHRRLTERVLSRKDSGDAQARVAAWAETGSEELALWQRTLAEMRASGFYDFAT
                     LSVGVESVRKLTD"
     gene            6601..7350
                     /gene="gpmA"
                     /locus_tag="VFB37_14905"
     CDS             6601..7350
                     /gene="gpmA"
                     /locus_tag="VFB37_14905"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HZO23790.1"
                     /translation="MPGKLILVRHGQSVWNVENLFTGWTDVDLSEQGRREAAQAGREL
                     LREKLDVDIAFTSVLKRAIRTLWLMLDEMDRMWIAVERSWRLNERHYGALQGLNKAQT
                     VEKHGAEQVKIWRRSYDVPPPKLAEDDRRHPRFDPRYADIDPAELPATESLKDTLQRV
                     LPYWHSRLAPELRAGRNVLVAAHGNSLRAMVKMLDDIPESAIVELNIPTGIPLLYELD
                     PNLKPTARRYLGDAQAIAAATEAVRRQTEKR"
     gene            complement(7355..7753)
                     /locus_tag="VFB37_14910"
     CDS             complement(7355..7753)
                     /locus_tag="VFB37_14910"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HZO23791.1"
                     /translation="MLVTVLAFVVAGGLIVGRIYFKIRKIQGSRAESWDARMIEQLRS
                     KGYAPFNDYKVDFFLALPDESACGAVRTRLEQQGFSVDTRPMPDTTELPFSLHATKLM
                     RLIVPDIQAQSRRMTDLATEFHGRYDGWAA"
     gene            complement(7768..>8342)
                     /locus_tag="VFB37_14915"
     CDS             complement(7768..>8342)
                     /locus_tag="VFB37_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO23792.1"
                     /translation="AKEGAIDGYLRELTGYLHGAISHFVRMDAPVIAAVNGTAAGAGV
                     GLVAMADLAICGRTARFSLAYTGVGLTPDAGTSFLLPRAIGAKRSMELMLLNRALSAE
                     EALEWGLVNQVVEDSQLIDEALVLAERLSAGAAGAIGKTKRLMAHSLGALESHMVLES
                     ETIASRAASEEGIEGIRAFLQKRAPEWGPR"
BASE COUNT         1439 a         2795 c         2715 g         1393 t
ORIGIN      
        1 cgctcggccg tcagctgttt gagcacaccg aggagcaggt cgatgttgag gctgcgctca
       61 tgtgcctcat cgatgatgag cgtgtcgtag cggcgcaggc gccggtcacc ctcgagctcc
      121 ctcagcagga tgccatcggt catcagcttg atgcgggtgg cggggccggt gcgatcggtg
      181 aagcgcacct gatagcccac caggccgctc acgagaggtg tgcccagctc ctgggcgaga
      241 cggccggcca gggcgcgcgc cgcgatacgc cggggctgcg tgtgcccgat cagaccggca
      301 ctcgcgcgcc cggcctccag acacaacttg ggcaattgcg tggacttgcc ggatccggtc
      361 gctccgcaga cgacgatgac ccgatgctgc acgagtgccg cgaggatctc ggcacgatga
      421 gcggtgatcg gtagagccgg gtcgtactcg agccgcaatg ccgctacagc actccgcgtc
      481 gcagctgatc cagctcgatc gactcgaaca gcgcgcggaa gttgccctcc ccgaaaccct
      541 cgttgccctt gcgctggatg atctcgaaga agatcgggcc gatgacattc tgcgtgaaga
      601 tctgcagcag cacgccctcg cccttgctcg gattgccgtc gatgaggatg ccgcgttgct
      661 gcagctcgcg cagcgactcg cggtgtccag gcacccgctc atcgaccgct tcgtagtacg
      721 tatcgggagt attctggaaa ctcactccct gcctgcgaag cgcatccacc gtccgataga
      781 tgtccttcgt ccccagcgcg atgtgctgga tgccctcgcc gtgatactcg cgaaggtact
      841 cctcgatctg actcttgtcg tcctgcgact cattgatcgg gatgcggatc ttgccgcagg
      901 ggctggtcat cgctcgtgag aacaggcccg tatgctgccc ctcgatgtcg aagtagcgga
      961 tctcgcggaa gttgaagagc ttgctgtaga acccggccca gacatccatg cgcccgcgca
     1021 tcacgttgtg cgtgagatgg tcgatgcaga cgagcccggc gccctcgttg agcgcggcag
     1081 aaggttcggg cctgaaatcc acatcgtaga tcgagcgctc accgtagaga tccaccagat
     1141 agatgagcga gccgccgatg ccttcgatgc tcgggatgtt cagctccatc ggaccggcgg
     1201 tctgcggtcc gggcttggcg ccgagctcga gcgcgcgccg gtaagcgaag gcggcgtcct
     1261 tgacccgaaa ggccatcgca caggccgagg ggccgtgctc gcgggcaaag ccctgcgcga
     1321 atgaatccgg ctcggcgttg atgatgaagt tgacatcccc ctgccggtgc agcgtgacgt
     1381 tcttcgatcg atggcgcgcg gccacaggaa atcccaggcg ctcgaaaaga gtgcgcagca
     1441 gttggggatc gggcgcggtg tactccacga actcgaaccc atccgtcccc atgggattgg
     1501 catatgtctc ggtcattggc tgattcctga atgagcccgg cgtgaggaat tataggcacg
     1561 gccttgtcct cgcggcgcgt gtcggcgaac catccaccga gcgaagcgct cggcggggct
     1621 gcgcgggctc ccgggggctc ccggggcctc atgtataatc cggcccccgt tcgacccggc
     1681 cggcagcgag cctgatctgg ccacgaaaca gagcatgagc acgcaagcgc cggtttcctc
     1741 ggaagacagc cgcctccttc acagcatccc cccctcccgg gtcgccttga tcgagggtat
     1801 cgcgctcgcg gcggcgcgca ggaaccgggg cgccctgcag caacgctatg tccgcgccta
     1861 cttccggggc gtcggcgagg aggatctcgc cgagcgtgcc ccctcggtac tggctcgcgc
     1921 cgccctcgag catctggaat tgggctccca gcgccggcgc ccgggccaac ccctggtacg
     1981 ggtcttcaat ccggatccgc agcgcgacgg attcgagtca ccgcacaccg tggtcatgct
     2041 cgtcaccgac gacatgccct tcctggttga ctctgttggc atcgtgttca gccgcgccga
     2101 catcgccgtg cacctgatca tccacccggt gctggagatc cagcgcgacg gccgcggccg
     2161 cctcaccggt ctcggcgcca atggatcgca gggggcgcat gcggaatcgt ggcagttcta
     2221 cgagatcgac cgccagaccg atccggccca gctcgagaag ctcaagagac ggatcgagac
     2281 gacgctcgcc gacgtacgca cggtggtcga ggactggcgc ccgatgcggg cgcagacgaa
     2341 gaccctcatc acggctctgg aaagcaaccc gccgccgctg tcggccgatg aggtgagcga
     2401 ggcccgcttg ctgctggatt ggatggaggg ccggcatttc gtctatctgg ggtatcgcta
     2461 ctatcgcctg gagcgcggcg caaacgagga tcgcctgata cccgatccgc gttccgggct
     2521 ggggatcctg cgcaccccgg catcgggctc gcgacccgcg cccatcgtgc tgcgcggaga
     2581 aatgcgggcg cgggcgcgcg agcgcgagct gctgatcctg accaaggcga actcgacggc
     2641 gacggtccac cgcggcgaat tcctggatta cgttggcgtc aagaacttcg atgagcgcgg
     2701 acgggtctgc ggcgagtacc gtttcctcgg actgtggacc tcgaccgcct atcacggcag
     2761 tccgcgcgat atccccgtcc tgcggcgcaa ggtgcagcag gtcatcgagt acttcgggct
     2821 ggatccccag agccatgatg gcaaggccgt gctcaatgtc ctggagacct atccacgcga
     2881 cgagctcttc caggcggcgg tggaggacct catccgtatt gcccgtggtg tggtcaatct
     2941 ctacgagcgc cgcaccgtcc ggctgctcgt gcgacgcgat ccctaccacc gcttttactc
     3001 ttgcctcatc tacgtgccgc gggaccgata taacactgag gtgcgtcagc gcatcgagca
     3061 gatcgttctc gagggcttct ccggccgcag cgtcgagact caggttcaga tctccgggtc
     3121 caaccacgcg cgcgtgcacg tcgtcgtgca caccgacccg gagagcaagc cgaaagtcga
     3181 tctggcagcg gtcgagcatc ggatcgcgga ggcggcgctc acctggacgg accggctgcg
     3241 cgatgtgttg atcgagaaca agggcgaggc ggcgggactg gcgctcgcga gccgttaccg
     3301 gcgctccttc gcgatggcct acgaggaaga cgtccagccg cgcgatgcgc tggatgacct
     3361 ggccgacctc gaagctctgc gcgagcagag cgcggcggcc gcctcggatg cgcgcggcgg
     3421 cacgctgcga ctgagcctgc atcgcccggc ccagcagaag cccgaacggg tgcatctcaa
     3481 gatcgtcaag ctcggcgatc cggttcccat ctccgacctg ctgccgatgc tggaaaactt
     3541 cggcctgcgg gtgatctcgg aacgccccta cgagctggcc tggcccgaag gcggcgcggc
     3601 ctggatccag gacttcgaac tcgagcaccg cgaccgcctg ctgatcgaca ttgcccgggt
     3661 ggaaaatcct ttcaaggacg cgtttctcgc ggcctggcgc ggcgaagtcg aaaatgacgg
     3721 cttcaatcgc ctgctgatcg tggccgggct cacggcgcgc gagatcgtgg tgctgcgcgc
     3781 gtattgccgc tatctgctgc agaccggcgt gcccttcagc caggcgtaca tggagcgaac
     3841 cctggctgcc aacgcagcca tcgcccgcaa cctggtgcga ctgttcgagc ggcgcttcga
     3901 cgccgacagc agcgcgcccg cgcgcggggc tgtgcgcgag gcggacaagc tggtggcgca
     3961 actgcgcacc gacctggatt ccgtgaccag cctcgatgaa gaccggatcc tgcgcgccta
     4021 tctgaatgtg gtcgaagcga cgctgcgcac caacttctat caacccgaca agccctatct
     4081 cgcggtcaag ctcgatccgt ccaaaattcc ggacctgccg ctgccgcggc cgaagttcga
     4141 agtgttcgtc tacagtccgc gtgtcgaggc cgtgcacctg cggatgggtt atgtggcacg
     4201 tggcggcatc cgctggtcgg accggcgtga ggacttccgt acggaaatcc tcgggctgat
     4261 gaaagcgcag aacgtcaaga acacgctgat cgttccggtc ggcgccaaag gcggcttcgt
     4321 gtgcaagaag ctgccgaccg gctcgcgcga ggaggtgcag gcggaggtca ttgcctgcta
     4381 ccagtattta atccgcggtc tcctcgatct gaccgacaac attgtcggcg gccgcatcgt
     4441 gccgcccgcg cgcgtggtgc gccgcgatcc agacgatgcg tacctcgtgg tcgccgccga
     4501 caagggtacg gcgactttct cggacatcgc caacgccatc tcgctggagt acggctactg
     4561 gctgggcgat gcctgcgcct ccggcggctc cgccggctac gaccacaaga agatggccat
     4621 caccgcgcgc ggcgcctggg aatgcgtcaa gcggcatttc cgggagatcg gcaccgacat
     4681 ccagaagaag gatttcaccg tcatcggcat cggtgacatg tcgggcgatg tgttcggcaa
     4741 cggcatgctg ctgtcccgcc acatccgcct gcaggctgcc ttcgaccacc ggcacatctt
     4801 cctcgatccc gctccggatg ccaccaccag ctttgccgag cggcagcgca tgttcgatct
     4861 gccgcgctcg agctgggacg attacgatcg caagaaaatc tcgcgcggcg gcggggtgtt
     4921 tccccgctcc gcgaaatcga ttgcgctcgc acccgaggcc gccgccatgc tcggactggc
     4981 cccgggcgct gcagcgccca acgaagtcat ccgcggaatt ctgcggatgc ccgccgacct
     5041 gctgtggaac gggggcatcg gcacgtacgt caaatcgagc gaggaatcca acgccgaggt
     5101 cggggaccgg accaacgatg cgctgcggat cgacggtcgg gagctgcggg tcaaggttgt
     5161 cggcgaaggc ggcaacctcg gcttctcgca gcgcggccgg gtcgagtacg cgctcgccgg
     5221 cggtcgtctc aacacggatt tcatcgacaa ctcggccggc gtgaacacct ccgacgtcga
     5281 agtcaacatc aagatcctgc tcaatccttt gatgcaggcc ggcaagctca cccgcggcga
     5341 gcgcaaccgg ctgctcgctc gcatgaccaa cgagatcgcg accctggtcc tgcgcaacaa
     5401 ctatctgcag agccaggcca tcagcacact cgagctgcag gcggccgccc gcctggccga
     5461 gtaccagcac ctgatccgca cgctcgagcg ctcgggcgag ctcaatcgcg ccctggagtt
     5521 cctgccggcg gacgatgagc tcgcggaccg gcgcaagcgc ggtgcgggcc tgacgcggcc
     5581 cgagctgtcg attctgctgg cctacagcaa gatctggctg acccaccatc tgctcgactc
     5641 ggacgtgccc gaggatcctt atctgtcctc cgagctggtg cgctacttcc ctgccccggt
     5701 gcaggagcgc ttcccgcgcg cgatcgctca gcatcgcctg cgccgcgaaa tcatcacgac
     5761 ggcgaccacc aacagtctcg tcaatcgaat ggggccggcg ttcgtgccgc gcgcccagga
     5821 agacaccggc gctgagcccg cgcagatcgc tcgcgcctac accgcagccc gcgagatatt
     5881 cgacatgcgc gagacgtggg ccgagatcga agcgctcgat acgaaggtcc cggcaaagct
     5941 gcagtactcg atggctttcc agaccagccg cctgctgcgc catgtcacgt actggctact
     6001 cgcccatcgc aagcgcgagc tgcacgtcga tggagccgtg gcggagttcc gccggggcgt
     6061 gcggcagctg caggccgagg tcacccacgt gctcgccggc tcggaccggg agcgattcga
     6121 gaaactccgc cgcgagcata tggaggccgg tgttccggcc aagctggcgg agcgcatagc
     6181 aagcctcgcg gccaacaacg ccgccctcga tatcgtggag atcgcgacac gccacgaggt
     6241 gagcgtggcg caggccgcgt gcgcctactt cgaggtcggc gcgcgggtcg ggctcgactg
     6301 gcttcgtgag cagatcgagc agctttcggt cgaggggccc tggcaggcga tcgcccggac
     6361 cggcctgcgc ggcgcggcca tgcgcattca tcgccggctc accgagcggg tgctgtcgcg
     6421 gaaggattca ggggatgccc aggcccgggt cgccgcgtgg gccgagacgg gtagcgagga
     6481 gcttgctttg tggcaacgga cactcgctga aatgcgtgcc tccggattct acgacttcgc
     6541 tacgctgtcg gtcggagtgg aatcggtccg taagctcacc gactgattgg aaaggtctct
     6601 atgcccggaa agctgatcct cgtccgccat ggccagagcg tgtggaatgt tgaaaacctg
     6661 ttcaccggct ggaccgatgt cgatctctcc gagcagggcc ggcgggaagc ggcgcaggcc
     6721 ggccgcgagc tgctgcgcga gaagctcgac gtggacatcg ccttcacatc cgtgctcaag
     6781 cgcgcaattc gcaccctctg gctgatgctc gacgagatgg atcggatgtg gattgcggtg
     6841 gagcgttcct ggcggctcaa cgagcgccac tatggagctt tgcagggact caacaaggca
     6901 cagacggtcg agaagcacgg cgcggagcag gtcaagatct ggcgccgcag ctacgatgtg
     6961 ccaccgccga agctggctga ggatgatcgg cgccacccgc gcttcgatcc gcgttacgcc
     7021 gacatcgacc ccgcggagtt gcccgccacc gagtcgctca aggacaccct gcagcgcgtg
     7081 ctgccctact ggcacagccg cctcgcaccg gagctgcgcg ccgggcgcaa tgtgctggtc
     7141 gccgcgcatg gcaacagcct gcgggcgatg gtcaagatgc tggatgacat ccccgaaagc
     7201 gcgatcgtcg agctcaacat tccaaccggc atcccgctgc tgtatgagct cgacccgaat
     7261 ctgaagccga ctgcccgccg ctacctcggc gatgcgcaag cgatcgcggc cgccaccgag
     7321 gccgtacgcc gtcagaccga gaagcgctga ggcctcaggc cgcccagccg tcgtaacggc
     7381 cgtggaactc ggtcgcgaga tcggtcatgc ggcggctctg cgcctggatg tccggcacga
     7441 tcaatcgcat gagcttggtc gcgtgcaggc tgaatggcag ctcggtcgta tccggcatcg
     7501 gccgggtatc gacgctgaag ccctgctgct cgagccgggt ccggaccgcc ccgcaggcgg
     7561 actcatcggg cagcgccaga aagaaatcca ccttgtaatc attgaagggc gcgtagccct
     7621 tggagcgcag ctgctcgatc attcgtgcat cccagctctc ggcccgtgag ccctggatct
     7681 tgcggatctt gaagtagatg cggccgacga tgagcccgcc cgcgaccacg aatgccagga
     7741 ctgtgacgag catgcctgcg ccggccgcta acgcggtccc cactccggcg cgcgcttctg
     7801 gaggaatgcg cggatgcctt cgatgccctc ttcgctggcg gcccgggaag caatggtctc
     7861 gctctcgagc accatgtggg actccagtgc accgagggag tgggccatca ggcgcttggt
     7921 cttgccgatt gccccggccg ctcccgccga gagacgctcc gccagcacca gcgcctcatc
     7981 gatgagctgc gagtcctcga ccacctggtt gacgagcccc cactccagcg cctcctcagc
     8041 cgagagggcc cggttgagca gcatcaactc catgctgcgc ttcgcgccga tcgcgcgcgg
     8101 cagcaggaac gacgtacccg cgtccggtgt caggcctact ccggtatacg cgaggctgaa
     8161 acgcgcggtg cggccgcaga tcgcgagatc ggccatcgcc acgaggccca cgcctgcacc
     8221 ggccgccgtc ccgttgactg cggcaatgac cggcgcatcc atcctcacga aatgcgagat
     8281 cgccccgtgc agatagccgg tgagctcgcg caggtagcca tcgattgcgc cctccttcgc
     8341 ga
//
LOCUS       DASRHN010000371         6036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1460785, whole genome shotgun sequence.
ACCESSION   DASRHN010000371 DASRHN010000000
VERSION     DASRHN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460785"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3095
                     /locus_tag="VFB37_14920"
     CDS             <1..3095
                     /locus_tag="VFB37_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23793.1"
                     /translation="VSTPLVGDGSITARVVSQTNTNAWAKAGVMIRETLAAGSTYAAV
                     EVTPAEGVSMQDRTLTNSNALSALGPFTKAPYWVRVVRSGSTFSGYASADGITWKLIS
                     STTITMASQVFMGMLVCSHSNGVLGTAVFDNVTATSGTAPPSDTQAPTVPTGLATSNV
                     TANSLTLSWAASTDLPNPGGTGVGGYYVYRNGNTTTPIATVTSGTSFNDSGLAAATSY
                     TYQVAAFDKASPVNVSAASTALHVTTQSSGGGTSWSSGDIGAVQASGSYTQSGGTFTV
                     SGSGADIWGTADEFQFVSTPLVGDGSITARVVSQTNTNAWAKAGVMIRETLAAGSTYA
                     AVMVTPGEGSAFQARTVTNGSAVSILGPFVKAPYWVRVVRAGSTFSGYASADGITWKL
                     ITSTTITMATQVYAGLIVSSHDNGALSTAVFDNVQAPVGGQGAVAITPRNAALTLSQS
                     QQFTAGSSGGGSVSWSVDGVAGGNGTVGTINSSGLYLPPATPGTHTVKATSTSQSTNF
                     ATAAVAVTDLAGIYTYHGDLARTGQNTHEYALTPATVQSPSFGKRWSCAVDGDVYAQP
                     LYVANLAIGGGTHNVLFVVTMHDSVYAFDADNSGCVTYWHVSFLGTGITSVSSTDNGC
                     TDVPNEYGITGTPVIDPTSRTLYLVALTKESGAFFQRLHALNIQTGAEQANSPVVIQG
                     SVTGAGGKSVTFSALQENKRGGLVLSNGGLYITWASHCDNGPWWGWLMRYDATSLAQT
                     AIFNTTPDGSEGGIWMSGGAVAVDSTGSLFFSTGNGTFDDTGSLVPPVAPRNDFGESF
                     LKVNPSSLTVEDFYTPSQEAAWSNQDLDIASSGVVVLPDGAGPSGHPNVLIGSDKQGH
                     LWMIDRTQMSRFSSTSDNVVQFLTLPPGCADECVLGTPAFWNDTVYFGIGFGNVVALP
                     LSGGLIPAGTGNVAAPSSHSTEKYGFPSPTPMISASPGGGGLVWVLDNQKNGTGGAAL
                     GPAVLRGYDASNLGKTVYDSSAASDTPANAVKFTVPVVANGHVYVGGRSQLTVYGLTP
                     "
     gene            complement(3111..3689)
                     /locus_tag="VFB37_14925"
     CDS             complement(3111..3689)
                     /locus_tag="VFB37_14925"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZO23794.1"
                     /translation="MMSEPPGATNQPAEALEQKSQGSANEQLFAALYNELRRIAERQL
                     RYNAGAPVSPTTLLHEAYLGMADKQQVFPDQARFIGYAARVMRNLIIDLVRERRALKR
                     GAGFHITQLSEEHERPGVDERELKNLSEALDELAVHDARLAEVVDLKYFCGLTFEDIA
                     AQRGTSTRTVQRDWEKARAILFQQLSNWNTQP"
     gene            3859..4797
                     /locus_tag="VFB37_14930"
     CDS             3859..4797
                     /locus_tag="VFB37_14930"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO23795.1"
                     /translation="MKIFVAGATGVIGRRAVPLMLEAGHDVTILARGGRRSRPRVSAG
                     VRTVQANLFDRAEVHRAVLGHESVVNLATHIPASTARMLLPGAWRENDRLRREASANL
                     AEAATLAGAARLVQESFALVYPDCGDGWIDEESPIEPVRYNRTVPDAERAAQRFTANG
                     GVGVVLRFSAFYGPDAVQTADMIKLVRKGWEPLPGAPNAYFSSISHDDAATAVLAALK
                     LPVGIYNVTDDEPVERRDFARTLAKAVGARPPRSLPAWVARLSGSMGKLMSRSERISN
                     RKLREASDWIPAYPSVRVGWPATVAALRAGQTRSLH"
     gene            complement(4839..5183)
                     /gene="arsC"
                     /locus_tag="VFB37_14935"
                     /pseudo
     CDS             complement(4839..5183)
                     /gene="arsC"
                     /locus_tag="VFB37_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065799.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance.;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
     gene            5270..>6036
                     /locus_tag="VFB37_14940"
     CDS             5270..>6036
                     /locus_tag="VFB37_14940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO23796.1"
                     /translation="MPLSIIATTWPLLLGMGVLMLGAGLQGTLLGVRATLEGFPTPVI
                     GIIMSCYYVGYLAGTRAAPRLVRKIGHIRVFAAFAAVASAAILVQGVLVHPVPWALMR
                     LLSGVCFAGIYVVAESWLNDRASQDNRGRLLAIYMAVLYVGLGAAQFLLIAASPRKAS
                     SFMLVSVLISLAMVPMVAWAQQTPGRALPRKVRYRDLYRNSPLGVVAISVSGMISSTL
                     FAMGPVYAHLRGFDNSGVATFMAVSILAAVLTQYPVGR"
BASE COUNT         1028 a         1915 c         1962 g         1131 t
ORIGIN      
        1 ttgtctccac gcctttggtg ggcgatggct cgatcactgc acgggtagtg agtcagacca
       61 ataccaatgc ctgggcgaaa gccggagtga tgatccgcga gactctcgcg gccggctcga
      121 cgtacgcggc cgttgaggtg actcctgcgg aaggcgtctc gatgcaggat cggacgctga
      181 ccaacagcaa cgccctgagc gcgctcgggc cgttcacgaa agctccgtac tgggtccggg
      241 tcgtgcgctc gggcagcacc ttcagtggct atgcctcggc cgatgggatc acctggaagc
      301 tgatcagcag caccacgatc acgatggcct cgcaagtgtt catgggcatg ctggtatgca
      361 gccatagcaa tggggtcctg ggcacggctg tcttcgacaa cgtgacggcg acttccggga
      421 cggcaccccc gtctgatacc caagccccga cggtgccgac cgggctggcc acctcgaatg
      481 tgaccgcgaa ctctctcacg ttgagctggg cggcatcgac cgatctgccg aatccgggtg
      541 ggaccggggt cggtgggtac tacgtttacc gcaatggcaa tacgacgaca ccgatcgcga
      601 cagtgaccag cggcacgagc ttcaatgata gcgggctggc tgcagccacg agctacacct
      661 accaggtcgc tgcctttgac aaggcctcgc cggtgaatgt ctcggcggcc tcgaccgctc
      721 tccatgtgac gacccagagc agtggcggcg ggacttcctg gagcagtggt gacattgggg
      781 cggtgcaggc gagcggctcg tacacccaaa gcggtggcac cttcacggtg agtggctccg
      841 gggcggatat ctggggcacg gccgatgagt tccagtttgt ctccacgcct ttggtgggcg
      901 atggctcgat cactgcacgg gtagtgagtc agaccaatac caatgcctgg gcgaaagccg
      961 gagtgatgat ccgcgagact ctcgcggccg gctcgacgta cgccgccgtc atggtgaccc
     1021 ccggggaagg atcggctttc caggcgcgta ccgtcacgaa cggcagcgcg gtaagcatat
     1081 tggggccgtt cgtcaaggcg ccatactggg tccgcgtggt acgggcgggc agcaccttca
     1141 gcggctatgc ctcggccgat gggatcacct ggaagctgat caccagcacc acgatcacga
     1201 tggcaacgca ggtttacgcc ggactcatcg tgagcagcca tgataatggg gccctcagca
     1261 cggctgtctt cgacaacgtc caggctcctg taggcggcca gggcgcggtc gcgatcacac
     1321 cgcggaatgc cgccctcacc ctctcgcagt cgcagcagtt cacggcgggc agcagcggtg
     1381 gcggatcggt gagctggagt gttgacggtg tagcaggggg caacggcacg gttggaacca
     1441 tcaactcgag cggtctgtat ctgccaccgg ctacaccggg cacgcacacc gtcaaggcga
     1501 ccagcacgtc tcagtcgacc aacttcgcga cggctgctgt cgcagtcacg gatctcgccg
     1561 gaatctacac gtatcacggt gacctggctc ggaccgggca gaacacgcac gagtatgcgt
     1621 tgacgcccgc cacggtccag agtccaagct tcggcaagcg ctggtcgtgt gctgtggacg
     1681 gcgacgtcta cgcccagccg ctctatgtag cgaatcttgc catcggcggc ggcacgcaca
     1741 atgtgctctt cgtggtcacg atgcatgact cggtgtatgc cttcgatgcc gacaactccg
     1801 gctgcgtgac ctactggcac gtgagcttcc tcggcaccgg aataacctcc gtgtcgtcga
     1861 cggacaacgg ttgtacggat gtcccgaatg aatacgggat cacgggcacg cccgtgattg
     1921 atccgaccag ccggacgctc tatctcgtgg cgctcaccaa ggagagcggc gccttcttcc
     1981 agcgcctgca cgcgctcaat atccagaccg gtgcggagca ggccaactcc cccgtcgtga
     2041 tacagggcag tgtgacgggc gcaggcggca agtcggtgac cttctccgca ctgcaggaga
     2101 acaaacgtgg tggcctggtg ctcagtaacg gtggcctcta catcacctgg gcctcgcatt
     2161 gcgacaatgg gccgtggtgg ggctggctga tgcgatacga tgccacgtcc ctggcgcaga
     2221 ctgcgatatt caacaccacc ccggatggct ccgagggagg catctggatg tccggtggcg
     2281 ctgtggccgt ggactccacc ggcagcctgt tcttctcgac cggtaacggg acgttcgacg
     2341 acaccggttc gctggtgcca ccggtggctc cgcggaacga ttttggcgag agcttcctca
     2401 aggtcaatcc cagctcactg acggtggagg atttctatac gccgtcgcag gaagcggcct
     2461 ggtccaatca ggatctcgac atcgcctcat ccggagtggt cgtgctgccg gatggtgccg
     2521 gcccgagcgg gcatccgaac gtgctcatcg gctcggacaa gcaggggcac ctgtggatga
     2581 tcgaccggac acagatgtcc cggttcagca gcaccagtga caacgttgtg cagtttctca
     2641 ctctgccgcc cggctgtgcc gacgagtgcg tgttagggac cccggcattc tggaacgaca
     2701 cggtctactt cgggatcggg ttcggaaatg tcgtggccct gccgctctcg ggtggcctca
     2761 ttccggcggg aaccggcaat gttgctgcgc cctcctcgca cagtacggag aagtatggat
     2821 tcccgagccc gacgccgatg atctcggcct ctccgggcgg cggcgggctc gtgtgggtcc
     2881 tcgataatca aaagaacggc accggtgggg cggcgctcgg gcctgccgtg ctgcgcggct
     2941 atgacgcctc gaatctcggc aagacggtct atgacagctc agcggcatcg gacactccgg
     3001 ccaacgcggt gaaattcacc gtgccggtcg tggcgaacgg acacgtgtat gtcggcggcc
     3061 gcagccagct gaccgtctat ggtctgacgc cctgagcgcc agcgcgtaga tcagggctgg
     3121 gtgttccagt tggagagctg ctgaaagagg atggcccgcg ccttctccca gtcccgctgc
     3181 accgtgcgcg tcgaggtgcc ccgctgggcg gcaatgtcct cgaaggtgag gccacagaag
     3241 tacttgagat ccacgacttc cgccaggcgc gcgtcgtgca ccgccagctc atcgagcgcc
     3301 tcggaaagat tcttcagctc gcgctcgtcc actcccggcc gctcgtgctc ctcggaaagc
     3361 tgcgtgatgt ggaatcccgc tccacgcttg agcgcacgcc gctcccgaac gaggtcgatg
     3421 atcagattgc gcatcacgcg cgcagcgtag ccgatgaagc gggcctgatc gggaaagacc
     3481 tgctgcttgt ccgccatgcc caggtaagcc tcgtgcagca gagtggtggg gctgacgggc
     3541 gcgccggcgt tgtagcgcaa ctgccgttcc gcgatgcgtc gcagctcgtt gtagagcgcc
     3601 gcgaagagct gctcgttggc actgccctgg ctcttctgct cgagtgcctc ggccggctga
     3661 ttcgtggctc cgggaggttc ggacatcatc tcgtcgtcaa tccctaaaga cttgccgcgg
     3721 cagaatgcac aaatgccgac agcgtggcaa tcgcattgtc gctgcaacga aaggggcgcg
     3781 ccgcattatg tcttctgcgg tcaacttaag cgtttccccc acagaaacag gaacagacct
     3841 tgcgcccccc ttgcgcgcat gaaaattttc gtagccggcg caaccggcgt cattggtcgc
     3901 cgtgccgtac cgctgatgct cgaggcaggt cacgatgtga ccattctcgc gcgcggcggt
     3961 cgccgctcgc ggccaagagt gtccgcgggc gtgcgcaccg ttcaggccaa tctgtttgat
     4021 cgggctgaag tccatcgcgc cgtcctcggt cacgaaagcg tcgtcaatct tgcaacgcac
     4081 attccggcaa gcaccgcgcg catgctcctg cctggtgcgt ggcgcgagaa tgatcgcctg
     4141 cgacgcgaag cttccgccaa cttggccgag gccgccacgc tggccggtgc tgcgcggctc
     4201 gtgcaggaat ccttcgcgct ggtctatccg gactgcggtg acggctggat cgatgaggaa
     4261 agcccgatcg agccggtccg ctacaaccgt acggtgcccg atgcggaacg cgcggcgcag
     4321 cgcttcaccg cgaacggagg cgtgggagtc gtgcttcggt tttcagcctt ctacggtccc
     4381 gatgccgttc agacggcgga catgatcaaa ctcgtacgca agggttggga gccgctgccg
     4441 ggagctccga acgcctattt ctcttctatc tcgcacgatg atgcggcgac tgcggtgctc
     4501 gccgctctga aattgccggt cggcatctac aacgtcacgg atgatgagcc cgtcgagcgc
     4561 cgcgacttcg ctcgcacgct ggcgaaggcg gtcggggcac gaccgccccg gtcactgccg
     4621 gcctgggtcg cgcgactgag cggctccatg ggcaagctca tgtcccggtc ggagcgcatc
     4681 tcgaaccgca agctgcgcga ggcgagtgac tggatcccgg cttatcccag cgtgcgcgtc
     4741 gggtggccgg caaccgtcgc ggcgctcagg gccggtcaga ctcggtcgct gcactgatgc
     4801 aataatcgcg acgcgggcct cacgcaagcc cgatctcagg gcagcagatc cagcacgagc
     4861 tcgggcggtc ggcacagggc ggtgccacgc ggtgtgacca cgatcgggcg gttcatgaga
     4921 atcggatgcg cgagcatgaa gtcgatcagc tcgtcgcggc tccattcatc atcgagcttc
     4981 agttctgcga agagtggctc cttggtgcgc aacaggccgc gcggtgcgat tcccatcgcg
     5041 gcgatcaggc tctgcaaggt gtcgcggctc ggcggctgct tgagatattc gatgatcgtc
     5101 ggctcgatgc cgcggctacg aatcagggcc agcgtgttgc gcgacgtgcc gcaggcggcg
     5161 ttgtgataga tcgtgaccgg cattgcggcc gcttgcggat cgcgtgctgc catgggttct
     5221 ccaatcgctg atatcatgcg cgcaggtgta cttgtcgatc taacgcacag tgcccctcag
     5281 catcatcgcc acgacgtggc cactgcttct gggaatgggc gtcctcatgc tcggtgcagg
     5341 gttgcaaggc accctgctcg gcgtgagagc caccctcgaa ggctttccca cgccggtcat
     5401 cggcatcatc atgtcatgtt attacgtggg atacctcgcg ggcacgcgcg cggccccgcg
     5461 cctggtccgc aagatcggcc acatccgggt attcgccgcg tttgcggcgg tcgcctccgc
     5521 ggcgatcctc gtgcagggcg tgctcgtgca tcccgtgccc tgggcgctga tgcgtctgct
     5581 ctcgggggtc tgcttcgccg gaatctacgt cgtggccgaa agctggctga acgaccgcgc
     5641 cagccaggac aaccgcggcc ggctgctggc aatctacatg gccgtgctgt acgtgggact
     5701 cggcgccgcg cagtttctgc tgatcgccgc gagtccgcgc aaagccagct cgttcatgct
     5761 cgtttccgtg ctgatttccc tcgccatggt gccgatggtg gcctgggctc agcagacccc
     5821 ggggcgcgcc ttgccgcgca aggtgcgtta tcgggatctg tatcgcaact caccgctggg
     5881 cgtcgtcgcc atcagcgtct cgggcatgat ctcgtccacg ctcttcgcca tgggaccggt
     5941 ctatgcccat ctacgcggtt tcgacaattc aggcgtagcc accttcatgg cggtgagcat
     6001 cctcgcagcg gtactcactc agtatccggt gggccg
//
LOCUS       DASRHN010000372        14433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_136658, whole genome shotgun sequence.
ACCESSION   DASRHN010000372 DASRHN010000000
VERSION     DASRHN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14433
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136658"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFB37_14945"
     CDS             <1..257
                     /locus_tag="VFB37_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23797.1"
                     /translation="TRNPAVFLDRLADMGTVERGKLADLVLLDANPLDDIANTRRIRA
                     VVLAGKYFDRQALDHMLKGVEEAAAQPERAALHKGGAPQG"
     gene            complement(356..2692)
                     /locus_tag="VFB37_14950"
     CDS             complement(356..2692)
                     /locus_tag="VFB37_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO23798.1"
                     /translation="MVHYAIKCTAIGVALGLSLGESVMAQQSRSDSTQLEEVIVTAEK
                     RESTVQTTPISLTAVTGVDIQDRGLTNLSSLVQSVPGVSIRTSGPGMAELEMRGIASV
                     GGNSPTVGFYYDETPLTAPAATNEGKIVISPALYDLNRVEVLRGPQGTLYGSGSMGGT
                     IKVVPNPPNPAAFDASAQATFGDTDHGGFDHAENGMVNLPFGEGLGAVRIVGSYDRES
                     GWVDRVVIQPGDFPNATGAGCAPFTGCVRGDVLAAPVQTVHHDVNDVERTTVRVSMLL
                     KPLEGLTITPSIFYETLRAGGLPYIDSNPGTDATYQPFDVAENYHDTFKLGSLNAKYQ
                     TDLFEISSTTSYWSRSEPLMQDTTESWTTALSLTTYLPSQGGLGPSRAIEFNPSHQTT
                     EELRLASVGNSRLKWLVGYFYSDFYSAWNITFPSTAPFLPAGPSTDLFSYFTPMPILQ
                     QSVFGELTYNITEPFAVTAGLRRYHYDARVRLDQFGSLTAPTTEAVSETDQGVTPKIS
                     ASYQLTKDFMLYATAAKGFRPGGGTGPVPTSGPLSCEAQLMQEYGSKSFVPGPVTFSS
                     DHVWSYELGEKLRFADNRVTINSSVYFESWIGVQQVNALSSCGYVYTANAGNAHVKGA
                     ELEIQAIVVPELVVSANAGYSDAALVSSSLVNAGFNPGTPIQEVPRWTSTASIAYRHG
                     LSNGLTLMARADNTYVASRTDETYSVNTLSGYDLANVRMGVESDRWSVALFVNNVADK
                     RALLNDITQDATNLPTFNRVALNQPRTAGIDFNYRFGK"
     gene            complement(2834..4402)
                     /locus_tag="VFB37_14955"
     CDS             complement(2834..4402)
                     /locus_tag="VFB37_14955"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZO23799.1"
                     /translation="MDEQQGHASRSRRPSGREAKRAARTARSAAFVPFITRQIPYYEV
                     LGEEGLELLEHNADTILQEIGIDFRDDPEALKLLKAAGADVQGERARFPRGMCRTIVQ
                     RSAPREFTQHARNPAHTVVIGGNRTVFAPAYGSPFVRSLDDGRRYAKIEDFRNFVKLA
                     YMSTSLHHSGGTICEPVDLPVNKRHFDMVYTHMKYSDKPYMGSVTHPDRAQDTVEMTK
                     ILFGDGYLDPATGKPNTCIISLINANSPLTWDATMLGALKTYARANQATIVTPFILAG
                     AMSPVTVAGTVSQTLAESLAGMTLAQLVNPGVPVVLGSFASSLSMQSGAPTFGTPEPA
                     LVLYVMAALARRLGVPFRSGGALCASKIPDAQAAFESANTLVPTCLGGVNFVLHTAGW
                     LEGGLAMGYEKFVMDADQAGMMHTLLAGVDLSDNGQALDAIREVGPGKHFLGCAHTQA
                     NFQTAFYRSPLADNNSYEQWEAEGATNMAKRANALWKKQLAEYEAPPLDPAIDEALLD
                     YMNRRKASFPDSNV"
     gene            complement(4515..5348)
                     /locus_tag="VFB37_14960"
     CDS             complement(4515..5348)
                     /locus_tag="VFB37_14960"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23800.1"
                     /translation="MSLVFAGVCSHAPGITGRSERADPAVRDAFYAGLGRMRLALEAQ
                     RPDVLIVIAAEHFANFFMNNMPSICVGMADQYEGPIEDEAWIRIKRTRVPGNAQLSKR
                     LIETVMQDVDLAYAQEWKFDHGISVPLHFLTPRYDLPIIPVNINCQGPPLIPLQRCYE
                     FGRALHRACEQMPERIALIGTGGISHWPATPDSGRINEAWDREFLERLLGNRREELLA
                     YRDDQVWREAGQGGFEIRTYIAVAGATEGHTGELWFYAPIPIFAVGCTVAVMNLPHSS
                     A"
     gene            complement(5345..5653)
                     /locus_tag="VFB37_14965"
     CDS             complement(5345..5653)
                     /locus_tag="VFB37_14965"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23801.1"
                     /translation="MSLYAVQKLIYQLNRDPEVRKRFELDLEGVLAQYELTEEERRCI
                     REPDIGLLYVMGVNGQLLMHFAALRGYAWNAYLEAMREGERKHGPVRTGLYTPARSRT
                     "
     gene            complement(5650..6168)
                     /gene="hpaR"
                     /locus_tag="VFB37_14970"
     CDS             complement(5650..6168)
                     /gene="hpaR"
                     /locus_tag="VFB37_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HZO23802.1"
                     /translation="MNRSVDTVMKPSVPMRAFSQSLPMALLRAREAVMRRFRPALRRQ
                     GVTEQQWRVLRALADAGVLEITALAQATFLLAPSLSRILPDMEARGLISRRQLDSDLR
                     RSAISLEPKGVRLIAAHAPDSERTYEAIARRFGPQRLEHLFRLLYELEGVLGEMAEES
                     GGQTAPRKRAAR"
     gene            complement(6161..7021)
                     /locus_tag="VFB37_14975"
     CDS             complement(6161..7021)
                     /locus_tag="VFB37_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO23803.1"
                     /translation="MKLLNFRTRGRESYGVLNSRGIVDLGARLHSEASDVLSLIRAGA
                     LASARAAAETDNVDFDLGEVQILNPAGRYTRYFCIGVNYPERNEEYKEGAEKPRYPSL
                     FMRTHTSFSAHEQPIVRPRESEQLDYEGEIVVVIGKGGRRIPQAEAMSHVIGYSCANE
                     GSVRDWLRHAKFNVTQGKNFDHSGALGPWLVTFDEVGPAPLRVRTRVNGEVRQDDTSD
                     RMIFSIPYLIGYLSTFCSLEPGDVILTGTPSGAGARFDPPRYLKPGDVVEVEVSRVGL
                     LRNAVVSDDE"
     gene            complement(7073..7984)
                     /gene="dapA"
                     /locus_tag="VFB37_14980"
     CDS             complement(7073..7984)
                     /gene="dapA"
                     /locus_tag="VFB37_14980"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZO23804.1"
                     /translation="MNKSLDRLKGSIPPLVTPIRDGEVDYEAYAQLVAFQIRDGSHGI
                     LVNGTTAEPSTLSTEERNRLVTVAVQVANKRVPVVAATGSQSLRETRELTEHAARVGA
                     DALLIVTPYYIRPPQRGLVAYYLEVAGISQLPWMIYHIPGRTAVSVTLDTVKELKARS
                     PQLVGMKQAVNDLAFVSECLGALGSEFRIFVGLEELSFPMMAVGACGLMNAVGNLRPR
                     ALADLCEAVWASELPKAQAMHQALLEINQAVFFDTNPIPMKYMMKRLGLLPTNEHRLP
                     MAPATAELEKRLDGVLRRAGLLKDHAA"
     gene            complement(8033..9553)
                     /gene="hpaE"
                     /locus_tag="VFB37_14985"
     CDS             complement(8033..9553)
                     /gene="hpaE"
                     /locus_tag="VFB37_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HZO23805.1"
                     /translation="MSASLESNLQKANACLARWRSSPLGHFIGGRPHPGSSGELLDNL
                     CPADGSVLNRMVCGDEHDVNAAAEAAAKAFPEWRARRGEERRTLLHRVADLIEARREE
                     IALIESMDTGQALRFMNSAALRAAENFRFFADRAPGAADGLSLPAAEHLNLTIRQPLG
                     PVAIITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPLTAMMLAEIMTEAGISPGVI
                     NLVNGTGEQAGRLLTEHPAIRATAFIGESRTGSLIMTQGAPTLKRVHLELGGKNPVIV
                     FEDADLDRALDAVVFMIYSLNGERCTSSSRLLVQRSIHDRFVERVAERVAHLKVGHPL
                     DPATEIGPLIHQRHLDKVLGYFPLARAEGAQLRAGGARIESLGCGYYVQPTLITGATA
                     SMRIAREEIFGPVLTVIPFDDDAEALAIANDTPYGLTAYIWTADGGRALWMARHVEAG
                     MVWVNSENNRHLPTPFGGVKASGIGRDGGDYSFDFYMETKNICLAQGTHRVPKLGV"
     gene            complement(9550..9951)
                     /locus_tag="VFB37_14990"
     CDS             complement(9550..9951)
                     /locus_tag="VFB37_14990"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HZO23806.1"
                     /translation="MPHILIEYSANLRGQLDLPRLLEAAHTSALATGVFPLGGLRTRA
                     YAAEHYRIADSHPDNAFIHIMVRVGHGRDLETRKRAGEAIFTAVCERLASLQERIPLG
                     LSLEMQEIDPVLTFKKNNLHEYVRRRAVGAK"
     gene            complement(10087..10530)
                     /locus_tag="VFB37_14995"
     CDS             complement(10087..10530)
                     /locus_tag="VFB37_14995"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZO23807.1"
                     /translation="MRFAQIEVGAVIDGGRRQVTEKEMIEFARRYDPQWYHTDPERAR
                     RSRWQGLIASGWLTCSIAMELLVRNLLADSESIGSPGIERLKWPTPLRPGDEVELRVE
                     VLRSSVTRSGALGVVRWRWVLQTQAGTTVLDCIATSLFELQASEA"
     gene            complement(10715..11590)
                     /locus_tag="VFB37_15000"
     CDS             complement(10715..11590)
                     /locus_tag="VFB37_15000"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZO23808.1"
                     /translation="MAESRAARTVARIVRGMATSDGAGVRLRRVIGQPALSELDPFLM
                     LDEFGTDKPDDYIAGFPEHPHRGFETVTYMLEGRMRHRDNHGHEGELGPGSVQWMTAG
                     RGIVHSEMPQQQEGRMRGFQLWLNLPARDKMTAPRYQEFGPERIASATPAKGVTVKVI
                     AGRVGSVVGPVSQPATDPTYLDVAIDNGASYVHELPFRHSAFVYVYEGALQVGGKSVA
                     AGELAVLTDGESIQLVGDGSAVSGPAAGETQTSRAILVAGQPLHEPVARYGPFVMNTR
                     EELQQAIEDFQRGRF"
     gene            complement(11603..12184)
                     /locus_tag="VFB37_15005"
     CDS             complement(11603..12184)
                     /locus_tag="VFB37_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZO23809.1"
                     /translation="MNLLHIDSSIQGDNSASRQLGQEIVARWREAVPGLRVKYRDLAA
                     DALPHFSARSLMRSDQEEAAADARALADFQAADVIVIGAPMYNFGIPSQLKAWIDRIV
                     VAGQTFRYTASGPEGLAKGKRVIVAISRGGVYAPGAPGEFAESYLKFVLGFIGIQDIT
                     FVRAEGLAVSAERREKSIQVARAAIAAPEAMAA"
     gene            12333..13232
                     /locus_tag="VFB37_15010"
     CDS             12333..13232
                     /locus_tag="VFB37_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO23810.1"
                     /translation="MQPLDDFYYFSQVIEHGGYAKAARALGIPKSRLSRHVSALEARL
                     GVRLINRSSRRLAVTDVGQEVHRHAQAMLAEAHAAVEAVEFARAEPRGLIKVSVPVAV
                     TQTALAEILPDFLVRYPAVRVQLHATNRRVDVINERFDAAVRVRTKPSGEDGLIMRSF
                     GEFRELLVASGAYLDQAGRLERPEQLTERETLGFAPEAEQQSWELFDRQCRGVRVEVT
                     PRLVCHDFVMLRAAAKAGLGIALLPESTVHADLESGVLQRVLPDWELPLGVLHVVFPS
                     RRGLLPSVRAFIDFLAERLTAAV"
     gene            complement(13142..14221)
                     /gene="rocF"
                     /locus_tag="VFB37_15015"
     CDS             complement(13142..14221)
                     /gene="rocF"
                     /locus_tag="VFB37_15015"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZO23811.1"
                     /translation="MMAVGLRTASLIGAPTDVGASDRGSSMGPEALRVAGIERALVRR
                     GLKVLDRGNLSGPPNPVRAPVQGYRHLAEVLEWNRQVHAAVYRDLADGHLPILLGGDH
                     SLSIGSISAVARHCRENGRALRILWLDAHADFNTCELSPSGNLHGMPVACLCGFGPAE
                     LVQLGGRTPAVDPRWIRQVGIRSVDSGEKRFVHEQELEVFDMRYIDEMGVRQAMELAL
                     ADLPTDTHLHVSFDVDFLDPEIAPGVGTTVPGGPSYREAQLCMEMIADTGRVASLDVV
                     ELNPALDVRNKTATLAVDLIESLFGKSTLMRRPAVPHSRTISEVDRLLEPRIAAQTAA
                     VSRSARKSMNARTEGSRPRRLGNTT"
     gene            complement(14218..>14433)
                     /locus_tag="VFB37_15020"
     CDS             complement(14218..>14433)
                     /locus_tag="VFB37_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23812.1"
                     /translation="EVTIFDSVGFALTDFSALRYLYRFNREQRTGVEEIDLVPDVTDP
                     KDLFGALKERTRWRAPAQVADSAAVRA"
BASE COUNT         2619 a         4663 c         4575 g         2576 t
ORIGIN      
        1 cgacccgtaa cccggccgtg tttctcgatc gactcgcgga tatgggcacg gtcgagagag
       61 gcaagctcgc cgatctcgtg ctgctggatg ccaatccgct cgatgacatc gccaacaccc
      121 gccggattcg tgcggtcgtc ctcgccggca agtatttcga ccgacaggct ctcgatcaca
      181 tgctgaaggg cgtagaagag gctgctgctc agccggagcg agcggccctg cataaggggg
      241 gcgcaccgca aggatgagcg cgatgatcgc aaaagaccat cgccgcgctg gtgccgttcg
      301 cgcccgctcg gttcaagacc gggatccgcc cgcgccacca gtgcgccggg ggaccttact
      361 tcccgaaccg atagttgaag tcgatgccgg cagtgcgggg ctgattcaac gccacgcggt
      421 tgaaggtcgg cagattggtg gcgtcctggg tgatgtcgtt gaggagtgcg cgcttgtcgg
      481 cgacgttgtt cacgaacagc gccacggacc agcgatcgct ctcgactccc atgcgaacat
      541 tggccaggtc gtaccctgac agcgtgttga cggagtatgt ttcatccgtg cggctcgcga
      601 cgtaagtgtt gtccgcacgc gccatgagag tcagtccgtt gctcagtccg tgccggtagg
      661 cgatcgaggc ggtcgaggtc cagcggggga cctcttgaat gggggttccc ggattgaacc
      721 cggcgttgac gagactcgag ctgaccagcg ccgcatctga atagccggcg ttggccgaca
      781 cgacgagctc ggggacgacg atcgcctgga tctcgagctc ggcccccttc acatgcgcat
      841 tgcccgcgtt ggccgtatag acgtatccgc aggaagacag ggcattgacc tgctggacac
      901 cgatccagct ctcgaaatac acggagctgt tgatcgtgac gcggttgtcg gcgaaacgca
      961 gtttctcacc cagctcgtag ctccaaacgt ggtccgagct gaacgtgacg ggccccggca
     1021 cgaatgactt actgccgtac tcctgcatca gctgggcctc gcaggacaga ggtcccgagg
     1081 tgggcacggg cccggtgccg ccgccgggcc tgaagccctt cgccgccgtg gcatagagca
     1141 tgaaatcctt ggtgagctgg taggaagcgg agatctttgg cgtcaccccc tggtcggttt
     1201 ccgacaccgc ctcggtggtc ggtgccgtga gcgaaccgaa ctgatccagt ctgacccgcg
     1261 cgtcgtaatg atagcgtcgc aggcctgcgg tcactgcgaa gggctccgtg atgttgtaag
     1321 tcagctcgcc aaacacggac tgctgcagga tgggcatcgg ggtgaaatag ctgaagagat
     1381 cggtggacgg cccggcaggt aagaacggtg ccgtcgatgg aaaggtgata ttccaggccg
     1441 aatagaagtc gctatagaag tacccgacca gccatttgag tctggagttg ccgaccgagg
     1501 ccagacgcag ctcctccgtg gtctggtgcg agggattgaa ctcaatggca cgggacggcc
     1561 cgagacctcc ctgggaggga aggtaagtgg tcaggctcag ggcagtcgtc catgactcgg
     1621 tggtgtcctg catcagcggc tcactgcggg accagtagga cgtagtcgaa ctgatttcga
     1681 acaggtccgt ctggtacttg gcgttcaagg agcccaactt gaaggtgtcg tggtagttct
     1741 ctgcgacgtc gaaaggctgg taggtcgcat cggtgcccgg gttgctgtcg atgtacggca
     1801 agccgccggc cctgagcgtc tcgtagaaga tcgacggtgt gatagtcaaa ccttccaggg
     1861 gcttgagcag catgctgacg cgaaccgtcg tgcgctccac atcgttcaca tcgtgatgca
     1921 ccgtctgcac gggcgccgcc aggacatcac cccgcacgca tccggtgaac ggcgcacaac
     1981 ctgctcccgt cgcgttcgga aaatctcccg gctggatcac tacccgatcg acccagccac
     2041 tctcccggtc ataggagccg acgatccgca ccgccccgag tccctcgccg aaaggtagat
     2101 tcaccatccc attctcggcg tgatcgaagc ccccgtgatc ggtgtcgccg aaggtggctt
     2161 gcgcggaagc gtcgaaggcg gcgggattgg ggggattagg cacgaccttg atcgtcccgc
     2221 ccatggagcc cgagccgtac agagttccct ggggcccgcg caacacctca acgcggttga
     2281 gatcatagag agccggggaa atgacgatct tgccctcatt cgtcgcagca ggcgccgtca
     2341 acggcgtctc gtcataataa aatccgacgg tgggcgaatt gccgccgacc gaggcgatgc
     2401 cgcgcatctc cagctccgcc atcccaggtc cgctggtgcg gatggagacg ccggggacgg
     2461 actgtacgag cgagctcagg tttgttagac cgcgatcctg aatatctacg ccggtgaccg
     2521 ccgtaaggct gatcggcgta gtctgcacgg tggactcgcg cttctccgcc gtgacgatga
     2581 cctcttcgag ctgcgtcgag tcggatcgcg attgctgggc catcacggat tcaccgagcg
     2641 agagacccaa cgcgacgcca atagcggtgc acttgatagc gtagtgaacc atgtgactcc
     2701 ccctgatcgc gcctccccgg agcgaatcca gtatagctca cggttaccgc aacaggggtc
     2761 gccaataggt cgggcgtacc aattctcgcg cgcccgatcg tgcgccggaa ttcgacttcg
     2821 cgcctcgagc ctactacacg ttcgaatccg gaaacgaggc cttgcgccgg ttcatgtagt
     2881 cgagcagcgc ctcatcgatc gccggatcga gaggcggagc ctcgtattcg gcgagctgct
     2941 tcttccagag cgcgttggca cgctttgcca tgttggttgc gccctcagcc tcccactgct
     3001 cgtagctgtt gttgtcggcg aggggcgagc ggtagaacgc ggtctgaaag tttgcctgcg
     3061 tgtgggcgca gccgaggaag tgcttgccag gtccaacctc acggatggca tcgagcgcct
     3121 gtccgttatc ggagagatct acgccagcga gcagagtgtg catcatgccg gcctgatcgg
     3181 cgtccatgac aaacttctcg tagcccatgg ccagcccgcc ttctagccag cctgcggtat
     3241 gcagcacgaa gttcacgccg cccagacagg tcggaaccaa cgtgttcgcg gactcgaagg
     3301 ccgcctgcgc atcgggaatc ttcgaggcgc aaagcgcccc cccggaacga aacggcaccc
     3361 ccaaacggcg cgccagtgcg gccatgacgt agagcaccag tgcgggttcg ggcgtgccga
     3421 aggtcggcgc gccggattgc atggacagcg aggaagcgaa gctcccgagc acgaccggca
     3481 caccggggtt cacgagctga gccagcgtca tgccagcaag ggattcggca agcgtctgag
     3541 agacggtccc ggcgacagtg accggcgaca tcgctcccgc cagaatgaat ggcgtgacga
     3601 tggtcgcctg gttagctctc gcataggtct tcaacgctcc gagcatcgtc gcatcccagg
     3661 ttagaggcga gttggcgttg atcaggctaa tgatgcaggt gttgggtttc ccagttgccg
     3721 gatcgagata cccgtcgccg aagagaatct tggtcatctc cacggtgtcc tgcgcccgat
     3781 caggatgggt taccgacccc atgtacggct tgtcgctgta cttcatgtgg gtgtaaacca
     3841 tgtcgaagtg ccgcttgttg acgggcaagt cgacgggctc gcagatggtg ccgccggagt
     3901 gatgcagcga cgtggacatg taagccaact tcacgaagtt gcgaaagtct tcgatcttgg
     3961 cgtagcgcct cccgtcatcc aaactccgca cgaacgggga gccgtacgca ggggcgaaga
     4021 ccgtacgatt gccgccgatc acgacggtat gcgcaggatt gcgcgcgtgt tgggtgaatt
     4081 cccggggagc agaacgctgc acgatggtcc gacacatccc gcggggaaag cgggcgcgct
     4141 cgccctggac gtcggcgcca gcggctttca gaagcttgag ggcctccggg tcatcgcgga
     4201 aatcgatccc gatctcctgc agaatggtgt cggcattgtg ctcgagcagc tcgagccctt
     4261 cctcacccag gacctcgtaa taggggatct ggcgggtgat gaacggcacg aaggcggcac
     4321 tgcgtgccgt gcgcgcggct cgcttggcct cccgtccgct cggacggcgt gatcgcgagg
     4381 catgaccttg ctgctcgtcc ataaccggtc tcctgggctt cgggcgtgcc gtgagcgggc
     4441 cggcggccat tatccccgtt ccacggaccg gtcaactgat caatttgcgg cgcggacttg
     4501 cgtgggcgcg gcattcacgc cgagctgtgc ggcaggttca tcacggcaac ggtgcacccg
     4561 accgcgaaga tgggaatcgg ggcatagaac cacagctcgc cagtgtgtcc ttccgtggcc
     4621 cctgcgacgg caatgtaggt gcggatctcg aagccgccct gccccgcctc gcgccagacc
     4681 tggtcgtccc ggtaagcgag gagctcctcg cgccgattgc ccagaagcct ctcgagaaac
     4741 tcccggtccc aggcctcatt gattcggccg gagtccggtg tggcgggcca atgcgagatg
     4801 ccgccggtgc caatcaatgc gatccgctcg ggcatctgct cgcacgcccg atgcagcgca
     4861 cggccgaact cataacagcg ctgcagcgga atcagcggcg gcccctggca gttgatgttg
     4921 accggaatga tcggcaggtc gtagcgcggg gtgagaaagt gaagtggcac actgatgccg
     4981 tgatcgaact tccactcctg cgcgtacgcc agatcgacgt cctgcatgac agtttcgatc
     5041 aggcgcttcg agagctgcgc attgccaggc acgcgggtgc gcttgatgcg gatccaggcc
     5101 tcatcctcga tcggtccttc atactggtcg gccataccga cgcagatcga gggcatgttg
     5161 ttcatgaaga aattcgcgaa gtgctcggct gcgatcacga tgagcacatc gggccgctgc
     5221 gcctcgagcg cgaggcgcat gcgcccgaga cccgcgtaga acgcatctcg gactgcggga
     5281 tcggctctct cgctgcgccc ggtgatgccc ggcgcatgac tgcatactcc ggcgaacacc
     5341 aggctcatgt gcggctccgg gcaggcgtat agagacccgt cctgacaggg ccgtgcttgc
     5401 gctcgccctc gcgcatggct tcgaggtaag cattccaggc gtagccccgc agcgcggcaa
     5461 agtgcatcag cagttggccg ttcacgccca tgacatagag cagcccgata tcgggctcgc
     5521 ggatgcagcg gcgctcctcc tcggtgagct cgtactgcgc cagcactccc tcgagatcca
     5581 gctcgaaacg cttacggacc tcgggatcgc ggttgagctg ataaatcagc ttctgcactg
     5641 cgtacaggct catcgtgcgg ctcgcttacg cggcgccgtc tggccgcctg actcctcggc
     5701 catctcaccc agcacacctt ccagctcgta caggagccgg aagaggtgct cgagacgctg
     5761 cgggccgaag cggcgcgcaa tcgcctcgta ggtccgctcg gagtcggggg cgtgagcggc
     5821 gatcaggcgc acgcctttgg gttcgagact gatggcgcta cggcgcaggt ccgagtcgag
     5881 ctgacgacgg ctgatgagac cgcgggcctc catatcggga aggatgcgcg acagactcgg
     5941 ggcgagcagg aaggtcgcct gcgccagagc ggtgatctcc agcactccgg catcggccag
     6001 tgcacgcagc acgcgccatt gctgctcggt gacgccctgt cggcgcagcg ctggtcgaaa
     6061 gcgccgcatc acggcttccc gggcgcgaag caaggccatg ggcaacgact gcgaaaaggc
     6121 tcgcatgggc acggacggtt tcatgaccgt gtcaacgctc ctattcatca tcactcacca
     6181 ctgcgttgcg cagcagcccc acgcgcgaca cttcgacttc gacgacgtcg cctggcttca
     6241 gatagcgcgg cggatcgaat ctcgctcctg cgccactcgg cgtaccggtg agaatcacat
     6301 cgccgggctc gagcgagcag aacgtggaca gatacccgat cagatacgga atcgagaaga
     6361 tcatccggtc gctggtgtcg tcctggcgaa cttctccatt gacccgggtc cgcacccgca
     6421 agggtgccgg gcccacctca tcaaaggtga cgagccacgg acccagggcc ccggagtgat
     6481 cgaagttctt gccctgcgtc acgttgaatt tggcgtgccg cagccagtca cgcaccgagc
     6541 cctcattggc acacgagtag ccgatgacgt ggctcatcgc ctcagcctga ggaatccgac
     6601 ggcctccctt gccgatcacg acgacgattt ccccttcgta gtcgagctgt tccgactcgc
     6661 gtgggcgcac gatcggttgc tcatgtgccg agaacgaggt gtgcgtacgc atgaagaggc
     6721 tcgggtagcg gggcttctcg gcgccctcct tgtattcctc gttgcgctcc gggtagttca
     6781 ccccgatgca gaagtagcgc gtgtaccgac ccgcaggatt caggatttgg acctcgccga
     6841 ggtcaaagtc aacgttgtcc gtttctgcag cggctcgcgc gctcgccagc gcgccggcac
     6901 gtatgagtga gagaacatca cttgcctcgg agtgcaggcg cgcgccgaga tcgacgattc
     6961 cgcgggaatt gagcactccg taggactcgc gcccccgggt acggaaatta agcagcttca
     7021 cagagcgctc tgcacataaa cggacatcga caccaactgc aaaccgcctg cctcacgcgg
     7081 catggtcctt cagcaatccc gcccgcctga gtaccccatc gaggcgcttt tccagctccg
     7141 ctgtcgccgg ggccatcggc aaacgatgct cgttggtcgg cagaagcccg aggcgcttca
     7201 tcatgtactt catcgggatc gggttggtat cgaaaaatac ggcctggttg atctccagga
     7261 gcgcctgatg catggcctgg gctttcggca gctcgctggc ccataccgcc tcgcacagat
     7321 cggcaagcgc gcgcggccga agattgccga ccgcgttcat cagcccacag gcgccgacgg
     7381 ccatcatggg aaagctcagc tcctcgagcc cgacgaagat ccggaactca gatccgaggg
     7441 cgccgaggca ctccgacacg aacgccagat cgttcaccgc ctgtttcatc ccgacgagct
     7501 gcggcgagcg cgctttcagt tccttgaccg tatccagagt cacgctgacc gcggttcgcc
     7561 ctggaatgtg atagatcatc cacggcagct gcgagatgcc ggccacctcg aggtagtaag
     7621 ccacgagtcc gcgttgcggg ggccggatgt agtatggggt gacgatcaac agtgcgtccg
     7681 cgccgacccg ggccgcgtgt tccgtcaact cccgcgtctc gcgcagcgat tgcgagccgg
     7741 tggctgcaac cacgggcacg cgtttgttgg cgacttgcac cgcaaccgtg accaggcggt
     7801 tgcgctcctc ggtgctcagg gtggagggct cggcggtcgt gccgttgacc agaatgccat
     7861 gtgatccgtc gcgtatctgg aaggcgacga gctgcgcgta cgcctcgtaa tccacctcac
     7921 catcacggat cggtgtgacg aggggcggaa tcgagccctt caaccggtcg agcgacttgt
     7981 tcaagatcta tctcctgcac gaagcgcctg cccgtgcgca tcggtgtgct cgtcagaccc
     8041 ccagcttcgg tacgcggtgt gtaccctgag cgaggcaaat attctttgtc tccatgtaga
     8101 agtcgaagct gtaatcccct ccatcgcgcc caatgccgct cgccttgact ccgccgaagg
     8161 gcgtgggcag atgccggttg ttctcggagt tcacccagac cataccggcc tcgacatgcc
     8221 gcgccatcca caacgcacga ccgccatccg ccgtccagat gtacgcggtc agtccgtaag
     8281 gcgtgtcgtt cgcgatggcg agtgcctctg cgtcgtcgtc aaaaggaatg acggtgagca
     8341 ccgggccgaa gatctcttcg cgagcgatgc gcatcgacgc tgtggccccg gtgatcagag
     8401 tcggctgcac gtagtagccg cagccaaggg attcgatccg agccccgccc gcacgcagct
     8461 gcgctccctc ggcgcgagcg agaggaaagt aacccagcac tttatccaga tgacgctggt
     8521 gaatcagagg gccaatctcg gtggcgggat ccagcggatg cccgaccttc aagtgagcca
     8581 cgcgctcggc gacacgctcg acgaaccggt catggatgga gcgttggacc aggagccgac
     8641 tcgaggaggt acagcgctcg ccattgaggc tgtagatcat gaagacgacg gcatccagtg
     8701 cccgatcgag gtcggcgtct tcgaacacga tcacgggatt tttgcccccg agctccaggt
     8761 gcacgcgctt cagcgtcggg gcgccctggg tcatgatgag gctgccggtc cgcgattcgc
     8821 cgatgaatgc cgtcgctcgg atcgccggat gctctgtcag caaacggccc gcctgctcgc
     8881 cggtcccgtt gacgagattg atgacgcccg gagagatccc ggcttcggtc atgatctctg
     8941 cgagcatcat cgctgtgagt ggactccact cggcaggttt gtgtacgacc gtacaacccg
     9001 cggcgagcgc cggagcgatc ttccaggtgg acagcatgaa aggcgtattc cacggcgtga
     9061 tgatggcgac gggaccgagc ggctggcgga tcgtcagatt gaggtgctcg gctgccggca
     9121 aggaaagacc gtcggcggcc cccggcgcgc gatcggcgaa gaagcggaag ttttcggccg
     9181 cccgcagcgc agcgctgttc atgaagcgca gagcctgccc ggtgtccatg gactcgatga
     9241 gcgcgatctc ctcgcggcgt gcctcgatga gatccgccac gcggtgcagg agggtccgcc
     9301 gctcctcgcc ccttctcgcc cgccactcgg gaaacgcctt cgcggcggcc tctgctgcgg
     9361 cgttcacatc gtgctcatcc ccgcagacca tcctgttgag cacgcttccg tccgcagggc
     9421 agaggttatc gagcaactca ccgctcgagc cggggtgggg gcggccgccg atgaaatgcc
     9481 cgagcggtga gctccgccag cgcgccaggc aggcgttggc cttctgcaga ttgctttcga
     9541 gtgaggcgct cacttggccc cgaccgcgcg tcgcctcacg tactcgtgga gattgttctt
     9601 cttgaaagtg agcacaggat cgatctcctg catctccagc gagagaccga gcggaatgcg
     9661 ctcctgcagc gaagcaagcc gttcgcatac ggcggtgaat atcgcctcac cagctctctt
     9721 gcgcgtttcg aggtcccgtc cgtgtcccac ccgaaccatg atgtggatga aggcgttgtc
     9781 cggatgcgaa tcggcgatgc ggtaatgctc cgcggcgtag gctcgcgtac gcaagccccc
     9841 caagggaaac accccagtcg ccagagcgct ggtgtgagcc gcctcgagca gccgaggcag
     9901 gtccagctgc ccgcgcagat tggcggagta ttcgatcagg atgtgcggca tcgccgactc
     9961 gcagcgtcgc ggacttgctt aacatcataa ttatctcgcg aagccaggct gtcaagcgcc
    10021 tgagacctcg cgcgacgcac ttccgagagt caggcgagtc gatagaaacg ccgcgccgcg
    10081 ggactgtcag gcctctgaag cctggagctc gaacaggctg gtggcgatgc aatcgagcac
    10141 cgtggtcccg gcctgagtct gcagcaccca ccgccagcgc acgactccca gggcgccgga
    10201 gcgcgtgacg ctcgatcgca gaacctcgac ccgcagctcg acctcatcac ccggacgcag
    10261 cggcgtgggc cacttcagtc gctcgatgcc cggggatccg atcgactccg aatccgccag
    10321 caggttcctc accagaagct ccatcgcgat cgagcaggtc agccagccgc tcgcgatgag
    10381 tccctgccag cggctgcgcc gggcgcgctc aggatcggtg tgataccact gcgggtcgta
    10441 acggcgagcg aactcgatca tttctttctc ggtgacctgg cggcggccgc cgtcaatcac
    10501 cgcacccact tcaatctgcg cgaaccgcat ccgggctcag cgcttggcgc tgtccgcgct
    10561 tgcccagagc ggcttcgctt ctgcggtttc gttcactcga gctcctcccg gcggcacggc
    10621 tctggcgaga cccgccaaac cttcgggaag cccccgccgc ccttgaccag actcgccgag
    10681 cctacgggaa gcctcgggcg gcatgcaatt gcgcttagaa gcgcccgcgc tgaaagtcct
    10741 cgatggcctg ctgcagctcc tcgcgggtat tcatcacgaa cggtccgtag cgcgccacgg
    10801 gctcgtgcag cggttggccg gccacgagaa tcgcccggct cgtctgggtt tctcccgcgg
    10861 ccggacccga cactgcggac ccgtcaccca ccagctggat gctctcaccg tcggtcagaa
    10921 cggcaagctc cccggccgcg accgatttgc ccccgacttg cagggcgccc tcatagacat
    10981 acacaaaggc agagtgtctg aatggcagtt cgtgtacgta cgaagcgccg ttgtcgatgg
    11041 ccacgtcgag ataggttgga tcggttgcag gctgggaaac cgggcccacg actgaaccca
    11101 ctcgtcctgc aatgaccttc acggtgaccc cctttgcggg cgtggcggat gcaatgcgct
    11161 ccgggccaaa ctcctggtag cggggcgcgg tcatcttgtc gcgcgccggc aggttgagcc
    11221 acagctggaa tccgcgcatg cgcccttcct gctgttgcgg catctccgag tgcacgatgc
    11281 cgcgcccggc cgtcatccat tgaacgctgc cgggacccag ctccccctca tgcccatggt
    11341 tgtcgcgatg acgcattcgc ccctccagca tgtacgtgac ggtctcgaag ccacggtgcg
    11401 ggtgctctgg aaagccggcg atgtagtcat ccggtttatc cgtgccgaac tcgtccagca
    11461 tcagaaacgg atcgagctcg gatagggctg gctgcccgat cacgcgcctg agcctgacac
    11521 ctgcgccgtc cgaggtcgcc atgccgcgta cgatgcgcgc cactgtgcgc gccgctctgc
    11581 tctccgccat gtcgctttct cctcaggctg ccatcgcctc cggcgcagcg atggctgcgc
    11641 gggcaacctg tatgcttttt tcccggcgct cggctgacac cgcgagcccc tcggcccgca
    11701 cgaacgtgat gtcctggata ccgatgaaac ccaacacgaa cttcagatac gactcggcaa
    11761 actcaccggg agcgcccggg gcgtaaacac cccctcgtga gatcgccacg atgacccgct
    11821 tacccttggc cagaccttcc ggtccagagg ccgtatagcg gaacgtctga cctgccacga
    11881 cgatgcgatc gatccacgcc ttgagctgcg agggaatgcc gaagttatac atgggggcac
    11941 cgatcacgat cacatcggct gcctgaaaat ccgcgagtgc tcgcgcgtca gcggcggcct
    12001 cctcctggtc cgatcgcatc aaggaccggg cggagaaatg cggcaaggca tcggccgcca
    12061 ggtcgcgata cttcactcgc aggccgggca ccgcctcgcg ccagcgggcc acgatctcct
    12121 ggcccagctg ccggctcgct gaattgtccc cctggatgct cgaatcgatg tgcagcagat
    12181 tcatgtgtcc tcccggactg gacgggccta cgaccgtgta gataatttag ttcctgcaac
    12241 gatccattgg aatccgggta gaataggaca gatcgttcca ttcgtagaac gatggcaggc
    12301 agtcttcgcg ggattaggaa cggagagccc acatgcagcc gctcgatgat ttctattact
    12361 tctcccaggt catcgaacat gggggctacg ccaaggccgc acgcgcgctc gggatcccca
    12421 aatcccgcct ctcacggcac gtttcggctc tcgaagcgag actcggcgta cggcttatca
    12481 atcgctcgag ccgtcgcctg gccgtgactg atgtggggca ggaagtgcac cgtcatgcgc
    12541 aggcgatgct ggcggaagcg cacgctgcgg tggaggctgt ggagtttgcg cgggccgagc
    12601 ctcgcggcct gatcaaggtc agcgtccccg tcgcggtcac tcagactgcc cttgcggaga
    12661 tcctccctga cttcctcgtg cgttaccccg cagtcagggt ccagctgcac gccaccaaca
    12721 ggcgcgtcga tgtcatcaac gagaggttcg acgccgcagt gcgggtgcgc acgaagccga
    12781 gcggtgagga tggtctcatc atgcgcagct tcggagagtt ccgcgagctc ctggtggcga
    12841 gtggcgcgta tctcgatcag gcgggcaggc tcgagcggcc cgagcaactg accgagcggg
    12901 aaacgctcgg cttcgcgcca gaggccgagc agcagagctg ggagctgttc gatcggcaat
    12961 gccgcggcgt gcgcgtcgag gtcactccac gactcgtctg ccatgacttt gtcatgctgc
    13021 gcgccgcggc gaaggccggg ctgggcatcg cactgctgcc cgagagcacg gtgcatgcgg
    13081 atctcgagag cggcgtactc cagcgcgtgc tgccggattg ggagttaccg ctgggagtcc
    13141 ttcatgtcgt attcccgagt cgccgtggcc tgctgccttc cgtgcgggcg ttcatcgatt
    13201 tcctcgccga gcggctgacg gcggccgtct gagccgcaat ccgcggctcg aggagccgat
    13261 cgacctcact gatcgttcga gagtgcggca cggcgggccg acgcatcagg gtgctcttgc
    13321 cgaagagcga ttcgatcaga tccacggcga gcgtggccgt cttgttgcgc acatcgagtg
    13381 ccgggttgag ctcgaccaca tccaacgacg ccacccggcc ggtgtcggca atcatctcca
    13441 tgcagagctg ggcttcgcga taggacggcc cgcccggcac ggtggtgccc acgcccgggg
    13501 cgatctccgg atcgagaaag tccacatcga agctgacatg caggtgcgtg tcggtcggca
    13561 ggtccgccaa ggcgagctcc atcgcctgac gcacgcccat ctcatcgatg tagcgcatgt
    13621 cgaacacctc gagctcctgc tcgtgcacga agcgcttctc gcccgagtcg acactgcgaa
    13681 tgcccacctg gcgaatccac ctgggatcta cggccggcgt gcgcccaccg agctgaacca
    13741 gctcggcagg accaaacccg cacaggcaag ctaccggcat cccatgcaga ttcccgctag
    13801 gcgaaagctc gcaggtgttg aagtccgcat gcgcgtccaa ccagagaatc ctcagcgccc
    13861 gaccgttctc gcggcaatgg cgcgcgaccg cactgatcga gccgatgctg aggctgtgat
    13921 caccacccag caggatgggc agatgtccat cagccaggtc gcgatacacc gccgcatgga
    13981 cctgccgatt ccactcgagg acctccgcga gatgccggta tccctgcacg ggcgcccgga
    14041 ccggattcgg cggcccgctg agattgccgc ggtcgagaac cttgaggccg cggcgcacga
    14101 gggcgcgctc gatgccggcc acgcgcagag cctccggccc catcgaggac ccccgatcac
    14161 tcgcgcccac atccgtgggt gcgccgatca ggctcgccgt gcgaaggccg actgccatca
    14221 tgcgcgcacc gctgcagagt cggcgacctg ggccggcgcg cgccagcgag tgcgctcctt
    14281 gagcgcaccg aacagatcct tcggatcggt gacatccgga acgagatcga tctcctcgac
    14341 gccggtgcgc tgctcgcggt tgaagcgata cagatagcgc agcgcagaga aatccgtcag
    14401 cgcaaatccc accgagtcga agatcgtgac ctc
//
LOCUS       DASRHN010000373         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_302411, whole genome shotgun sequence.
ACCESSION   DASRHN010000373 DASRHN010000000
VERSION     DASRHN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302411"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFB37_15025"
     CDS             complement(<1..236)
                     /locus_tag="VFB37_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23813.1"
                     /translation="MRLPELPPLSTLRSRLVPGQWDVLAFLLVAVFFIYLAEAAHGLT
                     QSLPRLEATPISLAPSALVGYAARTALRMLIAMVA"
     gene            357..1031
                     /locus_tag="VFB37_15030"
     CDS             357..1031
                     /locus_tag="VFB37_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO23814.1"
                     /translation="MRLLIVEDDNELREALTAMLEQSGYEVDAHVDGRIASNTLMSKE
                     YDLAIVDLMVPGVSGLTLVRTLRRQLSGLPILIVTARDSLEDRVSGLDAGADDYLVKP
                     FEMPELEARVRALLRRNRADRETEIRVGPLVLVPGEPRVSLGGTSVDLPASEMALLET
                     LATRLGRVVSKEAIADRLSRGGNPPSDTAIEICVHRLRRRLTPYGLKIRTLRGFGYLL
                     ETTVGE"
     gene            1024..2391
                     /locus_tag="VFB37_15035"
     CDS             1024..2391
                     /locus_tag="VFB37_15035"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HZO23815.1"
                     /translation="MNDRSLRVGLLARLGSVLLLLLALDAVACYLTALHFANLVYDRW
                     LIDSTRSLAQAVSAENGLIHFDVPRVALQIFQFDEVDHTYFQVFSKQRGYLAGDATLP
                     DESDVQINGIKLANGAVRGQPVRVVATRIGPPATTDTVTVTVAETLVKRQTLARDILV
                     GMVAPQIALLGIALLLAWQGISRGLKPLTDLAAQIEERDQNNLSPVPQTGLPREARIL
                     TSRINELLVRLSNAMRAQRRFVADAAHQLRTPLAAVMLHTERAERAQDTQSERDALRA
                     LHRSVERAARLSQQLLALARTDPEALSVLELKPTDLVSLARRVGEEWIPRALERDIDF
                     GLDVPESAVSVNGDPRLLGELLSNLIDNALRYGRSSGRVSVIVEGGHQPKLSVVDDGP
                     GIPEEERPRIFERFYRIPDSNGEGCGLGLAIVEEIARLHSATVEVGPGTEERGARFTV
                     TFQAM"
     gene            2545..>2977
                     /locus_tag="VFB37_15040"
     CDS             2545..>2977
                     /locus_tag="VFB37_15040"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23816.1"
                     /translation="MASSGGLEVQYLPLSGSGPARWDGVIGVATFDRALGTPDIPVAK
                     VATPVLGAAGEVCEVWKTGRAARSGVHGRVHYRHGSGVLFGCLTLEETQRPDSRSEAT
                     RPEESALHAATEEGYREIFATLDSLGYPYLLRIWNYLPEINL"
BASE COUNT          499 a          992 c          995 g          491 t
ORIGIN      
        1 gcgaccatgg cgatcagcat gcgcagggcg gtgcgggcgg cgtaccctac gagcgccgag
       61 ggtgcgagcg aaatcggcgt cgcctcgagg cgcggcaggg actgggtgag cccgtgggcc
      121 gcctcggcca gatagatgaa aaacaccgcc accagcagaa aggcgagcac gtcccactgg
      181 cccgggacga gccggcttcg cagcgtggag aggggtggca gctcaggcag acgcatatcc
      241 gctcaagttt gcccgaactg cctgggcatt ggcaccgtcg ggcaagcatc gggtgctcgg
      301 tccgccgcat ccgcaagggc tacaataggg ttcccgagct ggcaactgtc acacgaatgc
      361 gtctgctgat cgttgaggat gataacgagc tgcgcgaagc gctgactgcc atgctggagc
      421 agtccggcta cgaagttgat gcgcacgttg acgggcgcat cgcctccaac acgctcatgt
      481 cgaaggaata cgatctggcg atcgtcgatc tgatggtccc tggagtgagt ggcctcacgt
      541 tggtgcggac cttgcgtcgc cagctctcgg gactgcccat cctgatcgtg accgcgcgtg
      601 actcgctcga agatcgggtg tcgggattgg atgcgggcgc cgatgactat ctggtcaagc
      661 ccttcgagat gccggagctc gaagcgcggg tgcgtgccct cctgcgccgt aaccgcgctg
      721 atcgggaaac ggagatccgc gtggggccgc tggtgctggt gccaggcgag ccgcgcgtat
      781 cactcggcgg cacgagcgtc gatctgccgg ccagcgagat ggctcttctg gagaccctcg
      841 ccacccgact cgggcgcgtc gtcagcaagg aagcgattgc ggaccgattg agccgcggcg
      901 gcaatccccc ctcggacacc gcgatcgaga tctgcgtcca tcggctgcgg cgccgcttga
      961 ccccgtacgg cctgaagatc cggacactgc gcgggttcgg ttacctgctg gagacaaccg
     1021 tcggtgaatg atcgcagcct gcgggtcggg ctgctcgcgc gactcggctc ggtactgctg
     1081 ctgctgcttg cactcgacgc cgtagcctgc tacctcaccg cgctgcattt cgccaatctc
     1141 gtctacgacc gctggctgat cgattcgacg cgctcgctcg cgcaagcggt gagcgcggag
     1201 aacgggctca tccatttcga tgtgccgcgc gtcgcgctgc agattttcca gttcgacgag
     1261 gtcgatcaca cctactttca ggtcttctcc aaacagcggg gctatcttgc gggcgatgca
     1321 acgcttccag atgaaagcga cgtgcagatc aacggcatca aactcgccaa cggcgccgtg
     1381 cgcgggcagc cggtccgagt cgtcgccacc cgcatcggcc cgccggccac caccgatacg
     1441 gtcaccgtca ccgtcgcgga aaccctcgtc aagcgccaga cgctcgcgcg cgacatcctc
     1501 gtcggaatgg tggctccgca gatcgcgctc ctcggcattg cattgctgct cgcctggcag
     1561 ggcatctcga gagggctgaa accgctcacc gacctggccg ctcagatcga ggagcgcgac
     1621 cagaacaacc tctcgccggt ccctcagacg gggcttccgc gcgaggcccg gattctgacc
     1681 agccgcatca acgagctgct ggtccggctg agcaacgcca tgcgagcgca gcgccgcttt
     1741 gtcgcggatg cggcccacca gctgagaact cccctggcgg ccgtgatgct ccacaccgag
     1801 cgcgccgagc gcgcgcagga cacgcaaagc gagcgggacg cgctgcgcgc actgcatcgc
     1861 tccgtcgagc gagccgcgcg cctgagtcaa cagcttctcg ccctggcgcg caccgacccg
     1921 gaagcgctct cggtcctcga gctcaagccc accgacctcg tttccctcgc acgacgggtc
     1981 ggcgaggaat ggatcccgcg cgcgctcgag cgcgatatcg acttcgggct cgatgtgccc
     2041 gagagcgcgg tctccgtcaa tggcgatccc cgtctgctgg gcgagctcct gtcgaacctg
     2101 atcgataacg cgctccgcta tggaagaagc tccggacggg tctcggtcat cgtcgaggga
     2161 ggccatcagc ccaagctctc ggtggtggac gacggacccg ggatccctga ggaggagcgc
     2221 ccccgcatct tcgagcgctt ctatcggata ccggattcca atggcgaggg atgcgggctc
     2281 gggctcgcca tcgtcgagga gattgctcgc ctgcacagcg cgaccgtgga ggtgggcccg
     2341 ggcactgagg agcgcggcgc ccgcttcaca gtgactttcc aggcgatgtg aagcgagcgc
     2401 cctggcgggc agccggtgga cccgtggggg ccggacgatt aaagtgcgcc gcatgagcgt
     2461 tgatcgcacc gattcgcaac gtctgctcgc gctgctcgcc ggcgacccat atgcccctac
     2521 gccacagcgg atgaacgccg cctgatggcc tcgagcgggg gtctcgaggt ccagtatctg
     2581 ccgctgagcg gctccgggcc ggcccggtgg gatggcgtga tcggggtggc cacattcgac
     2641 cgtgccctgg ggacccccga cattcccgtc gcaaaagtgg cgacacccgt gctgggggct
     2701 gccggtgagg tgtgcgaggt ctggaagacc gggcgcgccg cccggtccgg cgtgcacggt
     2761 cgggtccatt accgacacgg ctccggggtg ctctttggct gcctcacgct cgaggagacg
     2821 cagcgccccg attcacgcag cgaagcgacg aggcccgagg aatcggcgct gcacgccgcg
     2881 accgaagagg gctatcgcga gatcttcgcg acgctcgatt cactcggtta tccgtacctg
     2941 ctgcgcatct ggaactacct gcccgagatc aatctcg
//
LOCUS       DASRHN010000374        13280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_472483, whole genome shotgun sequence.
ACCESSION   DASRHN010000374 DASRHN010000000
VERSION     DASRHN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472483"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..229
                     /locus_tag="VFB37_15045"
     CDS             14..229
                     /locus_tag="VFB37_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23817.1"
                     /translation="MSPPSSPDPSPPRPPPPQQPPRRGALVGLVLVVVLILAGLLLSR
                     LLGRAARLQDCVISGRTDCSSANSSSR"
     gene            complement(232..990)
                     /locus_tag="VFB37_15050"
     CDS             complement(232..990)
                     /locus_tag="VFB37_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HZO23818.1"
                     /translation="MPLPVPRTGNPGLNERTFASQPRAAAYGERMTLQGSINKSFLLL
                     VVLLAGALWPWSQYLATGDASVVSSSVLIGAVGGLVLAVIISFKATTAPYLALPYAAL
                     EGLAIGGISALLERRYPGIALQAVALTFGVLAVLLVAYRMRIIRVTERFRAIVIGATG
                     AIALVYVVSMALGFFHINVPVLNAATPLGIVVSLLIVGVAALNLVLDFDLIESGVAAG
                     APRYMEWYAAFGLLVTLVWLYMEILRLLSKVRRQ"
     gene            complement(1061..1624)
                     /locus_tag="VFB37_15055"
     CDS             complement(1061..1624)
                     /locus_tag="VFB37_15055"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HZO23819.1"
                     /translation="MHISSWRLTMAALLGAAISLLAEAADLVKPDIRPGLWEVTTSPQ
                     MSGQIPIPEEELAKLTPEQRARMEEALKKGMSNAAQPRVYKECMTKEKIAKGFDAADP
                     REAGSCKRNVLTSSSSELHIREDCNRPDGKSTVDVHFQLSGGVQMSGTVNAVMTSGSR
                     TMTMNSKIEGKWVAASCGSVKDAEKEK"
     gene            complement(1672..2502)
                     /locus_tag="VFB37_15060"
     CDS             complement(1672..2502)
                     /locus_tag="VFB37_15060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZO23820.1"
                     /translation="MLSWHRVLVLATLASALPGCASLDDKQWYQNAKTASTKAFNVAA
                     DSTSKALQRTQHYLAEQDVLMKFHDAGEHSEKEVLEVLHRSGIAHGRSGAGGTAKSSG
                     TPQPATHATGSTQAGTHASAPTSVPIQYAGALRWPVQAGIVSSKYGPRWGKMHKGLDI
                     AADVGEPVLAIAGGDVIYAGDGLHGYGNVVIIQHDKQMTSLYAHNSELKVHQGDRVKQ
                     GAVIALLGNTGHSTGPHVHFEIREGDSAVDPAGVLPASMVAGGRGQPQEPRQRVTAGQ
                     "
     gene            2619..3182
                     /locus_tag="VFB37_15065"
     CDS             2619..3182
                     /locus_tag="VFB37_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23821.1"
                     /translation="MNALLRRIDGLSWLAMACIVVGWFGLDTLIATFGPVRHSAHFYD
                     LPAILLDPRRLLFGPDVGFSAGSFAFGLLSLAMIVLSVLPRLGVPRARWLLILAPLLW
                     MTLCGIVLYVKASSAHISAPEQMGRMGGYLASWANGAMRWTGDVAARHITIGPGAYLA
                     FAASCWLALKGASGLRESDTVPAVPTD"
     gene            3245..4060
                     /locus_tag="VFB37_15070"
     CDS             3245..4060
                     /locus_tag="VFB37_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZO23822.1"
                     /translation="MHDVVTEEQLPELLRLAEAAARSASELILSGFRSPRLAVERKGD
                     GSPVTPFDREAERTIRAFIAANQPRPWPVLGEELGDDTGQSRYRWVVDPIDGTLGFTR
                     GLPNFGTLLAFEDALAGRALVGVIHLPAMSETYRAARGLGAWCADERLSVAPPRALAD
                     CLISAPPQSQFQLAGTAASHAKLRAQVPHLRCFGDCWAHAMVARGPIDALAEFNLARW
                     DIAATEVLVEEGGGRVFIRDARDVHGKYDIVIGSPQAAAAVAELLQFGAPAQV"
     gene            complement(4068..4787)
                     /locus_tag="VFB37_15075"
     CDS             complement(4068..4787)
                     /locus_tag="VFB37_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HZO23823.1"
                     /translation="MTPTDLLFREDAYLKTATARIVAQSDRGLELDRTIFYPLGGGQP
                     GDTGALIRENGERIPVIDTRKGSAINSVVHVPSSSEPMRAQVGEPVTLELDWSRRYSL
                     MRLHTALHVMSCVVTAPVTGGNISPDKGRLDFDIELSLLDAARIERETNALIARALDT
                     ETVWITDEELDARPELVKTMSVQPPRGAGRIRLVRIPGIDLQPCGGTHVRNIGEIGAI
                     RVVRIRNEGKHNKRVEIALAT"
     gene            4880..5416
                     /locus_tag="VFB37_15080"
     CDS             4880..5416
                     /locus_tag="VFB37_15080"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HZO23824.1"
                     /translation="MKAPGRLKWVLVSLLGSSVGFGAAERPALAVLDVELTGDLGGPQ
                     FEAEHAVRLRMASERLRQELARSGLYRLVDLAPARGLILRLQSQQRYLHDCNGCDLDV
                     GRQLGADQVLVAWVDRVSGLILSLTYEIHDVASGRILARKSFDFRGDNDAAWTHAIGY
                     MVRDLEASSPSGPDPASH"
     gene            complement(5545..6840)
                     /locus_tag="VFB37_15085"
     CDS             complement(5545..6840)
                     /locus_tag="VFB37_15085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZO23825.1"
                     /translation="MTDAAWIGAAVSAARPQAVAALLRYFRDLDVAEEAFQEACLRAL
                     KSWPVNGPPRDPAAWLIFVGRNSALDEMRRRSKLDSLADEELVSDVEDTEAALAEKLD
                     SSAYHDDVLRLLFICCQPDLPATQQIALALRIVSGLSVKEIASAFLVSEAAMAQRITR
                     AKRAMADASVAFETPGPVERAQRLTAVAAMIYLLFNEGYSASGGEAHVRLPLCEEAIR
                     LARLLLRLFPGQGEIIGLTALLLLQHARAAARLDANSNIVLLEDQNRRLWNHELIAEA
                     LVLVQKARRLEQPGPYQLQAEIAAVHARALRWTETDWAEIDAIYSVLEQVQPSPVVTL
                     NRAVAVSKVHGPGAALKMIEPLAEPLAAYFYFFGVKGALLTELRRTAEARIAFTQAIA
                     LARTAAEAAHIRANLDRLASMDAEHPQDEKQPHREERGP"
     gene            complement(6837..7199)
                     /locus_tag="VFB37_15090"
     CDS             complement(6837..7199)
                     /locus_tag="VFB37_15090"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZO23826.1"
                     /translation="MFYCILCYNLESELEAWPREKEAAVLAQREVVAQHLAAQGRLGP
                     VARLMPTTTAMTMRASREPVVLDGPFAETKEQLLGFYMVDCRTLEEAIAVARNLVGDT
                     GALEVRPVATFYPGVGLQ"
     gene            complement(7314..7928)
                     /locus_tag="VFB37_15095"
     CDS             complement(7314..7928)
                     /locus_tag="VFB37_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HZO23827.1"
                     /translation="MTLRRLFPTLVYQARLRSRERARLEPRLLRECRQLRADDTAGRK
                     WSKRNYLGGYTSYGSVSRLHQLSPTFAELERHLGRHVRLFARSLEFDLRRRPLTMTDC
                     WVNIMPQRVAHGLHLHPLSTLSGTYFLRTPAGCPGLKLEDPRLDRFMAVPPRIGARIH
                     NRAWIVIPAQAGSLVLFESWLRHEVAPNPSASERISISFNYGWF"
     gene            complement(7925..8449)
                     /locus_tag="VFB37_15100"
     CDS             complement(7925..8449)
                     /locus_tag="VFB37_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HZO23828.1"
                     /translation="MKLFSRSLLDELAARAAESPRLRANHNIHLSAADRVQRFFIAAN
                     QASYFRPHRHHTKSELAMVLRGRFDILTFDAAGTVTARHAIGEGAPSIGFETPQETWH
                     TLIAMADGSTFLEIKEGPYDAASAAEFAAWAPPEGDRAAGPFLEWLRHAQPGTLVAPA
                     VQHLAAAPDSRTPG"
     gene            8531..9352
                     /locus_tag="VFB37_15105"
     CDS             8531..9352
                     /locus_tag="VFB37_15105"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZO23829.1"
                     /translation="MSATQNERADRFRALHAAGTFVIANAWDGGSAKVLAGLGFKAVA
                     TSSAAFAWTLGRHDGQVSREEALAHARLIAAATALPVAADLEKGFGDSPEAVAETIRL
                     AARTGLVGGSIEDASGDAHAPIYEAALAAERVAAAVEAARALPFSFTLTARAENFLCA
                     KPDLDDTIRRLQAFERAGADVLFAPGLPDLAAVRAVCSAVSRPVNFMVGMRGRSFSFA
                     ELAEAGVRRISLATSLYRAAMAGLMSAAREVRDRGTFGFVDSPVNTSELDAFLRE"
     gene            9440..10051
                     /locus_tag="VFB37_15110"
     CDS             9440..10051
                     /locus_tag="VFB37_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23830.1"
                     /translation="MRASAFRRVGTRLVVPVALTSFLCGACLAAEAGSPEDAAQPAVW
                     TTKKLDFVFQGFTAKYSCDGLKDKVTRALLQLGAQKKDLKVREMGCVNGFGRPDPFPG
                     VSIKMSVLQPASGSTDSSQPPVAAHWKPVKINLRDINDYPNDSGECELYEQIREKVVP
                     LFSTRNVDLSGICVPHQESAAGPTLKLEVLQADAKDTRSASAK"
     gene            complement(10055..11311)
                     /locus_tag="VFB37_15115"
     CDS             complement(10055..11311)
                     /locus_tag="VFB37_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO23831.1"
                     /translation="MLGSLTEATATLSKAASASRTTFSILAAIGFCHLLNDMMQSLLP
                     ALYPMLKSSYALSFGEIGILTLTFQITASLLQPVIGSYTDRSPRPYSLAMGMAFTLVG
                     LVLLAFASSYALLLFAAALIGTGSSVFHPESSRVARMASGGQHGLAQSVFQVGGNVGS
                     ATGPLLAAFIVLPRGQSSVAWFSGAALLAMFVLFQVGHWYKAHGLASLKPRASPIDKP
                     MTIDRRRMRGAIAVLLALIFSKYFYLASLTSYYTFYLINRFHVSVQSSQLHLFVFLGA
                     VAVGTMIGGPLGDRVGRRYIIWVSILGVLPFSLLLPHANLFWTGVLSVPIGLILASAF
                     PAIVVYAQELMPGRTGTVAGLFFGFAFGMGGIGAAVLGKLADLWGITTIYQICAFLPL
                     IGVLAGLLPDVEKHDPAPRAQAAGAA"
     gene            complement(11455..>13280)
                     /locus_tag="VFB37_15120"
     CDS             complement(11455..>13280)
                     /locus_tag="VFB37_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO23832.1"
                     /translation="PISSIAAVRLVGWDEKDAGFIDNVPGTVTYVPQGGPPTFTQNNA
                     AQVKKDYNDVETKGGRAALKVDLTDNWTVTPQVMGQSSDTHGFFAYNPKLGDLEVQKY
                     FPEEIHDSWVQSALTIEGKINNFDLVYAGAYLTRNTHENEDYTDYSLAYTAAYTFDSY
                     ATYFRDNAGKLINPAQYIVGRDHYTKLSHELRVTSPKEYPVRVTAGLFLQRQVHEILQ
                     DYTIPGGPAMNDPLASTGPFNLSVPGWPGTIWLTDEERVDRDAAVFGELSWDITSALT
                     LNAGLRHWRFDNTLQGFYGFNSTYSSHEGIATCFTPFVPFHGAVCQDLNGRSNDTGNS
                     PKINLTYKIDPDHMVYATWSKGFRPGGVNRNGGGKLPPYKPDFLTNYEIGWKTTWLDN
                     SLRWNGAIFDEKWKDFQFAFLGPNALTIINNAGNAEIKGVESEMEWRVANPFTLSASA
                     AYLNAKLTQTYCDAIGNGGTLADCLVPGYEGYAPSGTQLPVTPKFKANLTGRYTIPLP
                     NEFQATLQASETYVGARWPDLRIVDRDAFGEMPSYAVTDLSAELARNSWTATLFVSNA
                     FDKRAILDRTASCGVLQCGTVAIYNTPNVPRMIGLKFGQRF"
BASE COUNT         2269 a         4172 c         4415 g         2424 t
ORIGIN      
        1 cgaatgaaga ccgatgagcc cgccgtcatc cccggatccc tccccgccgc gcccaccgcc
       61 gccgcagcag ccgccccgcc ggggagcgct cgttggcctg gtcctcgtcg tggtgctgat
      121 cctcgccggc ttgctgctgt ctcggctgct cggccgagct gcacggctgc aggactgcgt
      181 catctctgga cgcaccgatt gctcatccgc caactcgagc tcgcgatgag gctactgccg
      241 cctgaccttg ctgagtagcc gcaggatctc catgtaaagc cagacgagcg tgacgaggag
      301 tccgaaggcc gcataccact ccatgtagcg gggtgctccg gcggccaccc cggactcgat
      361 caggtcgaaa tccagcacca aattcagcgc cgcaaccccg acgatcagca gtgagaccac
      421 gatgcccaga ggtgtggcgg cattcagcac gggcacgttg atgtggaaga aacccagagc
      481 catcgagacg acgtagacga gagcgatcgc gccggtggcg ccaatgacga tcgcgcgaaa
      541 gcgctcggtg acccggatga tgcgcatccg gtaggcaacc aacagcacgg ccagcacccc
      601 gaacgtgagg gcgacagcct ggagcgcaat tcccggatat cgcctctcga gcaaggcgga
      661 tatgccgccg atcgccagtc cctcaagtgc ggcgtacggc agcgccaggt agggagctgt
      721 cgtcgccttg aagctgatga tgacggcgag aacgaggcca ccgaccgccc cgatcagcac
      781 cgaggaggag acgaccgagg catctccggt cgcgaggtat tgcgaccagg gccagagcgc
      841 gcccgccagc agcacgacga ggagcagaaa tgatttgttg atcgagccct gcagcgtcat
      901 gcgctcgccg taggcggcgg cgcggggctg gctcgcgaat gtcctctcgt tcagtccggg
      961 attacctgtt cgcgggacgg gcagcggcat cgggatctcc tgtcggggct tcgaggacga
     1021 ttgtagtcga ccgggaacgg ctgggcaggt tccgcgacgg ttacttctcc ttttccgcgt
     1081 ccttgaccga gccgcagctc gcggcgaccc acttgccctc gatcttgctg ttcatcgtca
     1141 tcgtcctgct gccggaggtc atgactgcgt tgacggtgcc actcatttgc acccctccgg
     1201 atagctggaa gtgcacgtcg acggtgctct taccatcggg tcggttgcag tcctcgcgga
     1261 tgtgcagctc gctgctggag ctggtgagca cgttcctctt gcagctaccg gcctcgcgcg
     1321 gatctgctgc atcgaaaccc tttgcgatct tctccttcgt catgcattcc ttgtagacgc
     1381 gaggttgagc tgcgttgctc atgcccttct tgagggcctc ttccatccga gcccgctgct
     1441 cgggagtgag cttggcgagc tcctcctccg ggatgggaat ctgtccgctc atctgcggac
     1501 tggtcgtgac ctcccaaagg ccagggcgga tgtcgggctt caccaggtcc gcagcctcgg
     1561 caagcaggct gatcgctgcg ccgagtaggg cggccatggt gagtcgccaa gaggagatat
     1621 gcatgagagg gctcctgccg agcgcccgca ggcggcgcgt tgttcattca atcactggcc
     1681 ggcggttacc cgctggcgcg gctcctgggg ttgcccccgg ccgcccgcca ccatggaggc
     1741 cggcagcaca ccggccggat ccacggccga atcgccttca cgaatctcga agtggacgtg
     1801 cggcccggtg gagtggccgg tgttgccgag caatgcgatc acggcacctt gcttcacacg
     1861 atcgccctga tggaccttca gctcgctgtt gtgtgcatac agcgaagtca tttgcttgtc
     1921 gtgctgaatg atcacgacat tgccgtagcc gtgcagaccg tcgcctgcgt aaatgacatc
     1981 gccgcccgca atggcgagca caggctcgcc gacatccgcc gcgatgtcga gacccttgtg
     2041 catcttgccc caccgcggac cgtacttcga gctcacgatg cccgcttgca ccggccagcg
     2101 aagcgcgccg gcgtactgga tcggaaccga ggtcggggcg cttgcgtgcg tgcctgcctg
     2161 agtgctcccg gtagcgtggg tcgccggctg cggagtgccg cttgacttcg ccgtgccgcc
     2221 cgcgccgctc ctgccatggg cgattcccga gcgatgcagg acctccagca cttccttctc
     2281 gctgtgctcg ccggcatcgt ggaacttcat gagcacgtcc tgctcggcga gatagtgttg
     2341 ggtgcgttgc agcgctttgc tggtggagtc ggcggctacg ttgaatgcct tggtggaggc
     2401 cgttttcgca ttctgatacc actgcttgtc atcgaggctc gcgcaaccgg ggagggcgct
     2461 tgccagggtt gccaggacga ggacccgatg ccagctcaac atagtccgct tcgcacactc
     2521 gaccgcgcgc cgggtgcctc ttgtacttct accgagaatc taccacgcac gccggggtgc
     2581 tgcacgccca cgtgaatgct gccagactag gcctcagcgt gaatgctctg ctgcgcagaa
     2641 tcgatgggtt gagctggctt gccatggcct gcatcgtcgt gggctggttc gggctggaca
     2701 ccctcatcgc cacgttcgga ccggttcgcc acagtgcaca cttctacgat cttccggcca
     2761 tcctcctgga tccgcggcgt ctgctgttcg gccccgatgt cgggttttcc gcgggcagtt
     2821 tcgcattcgg gctgctgagc ctggcgatga tcgtgctgtc ggtgctgccg cgcctgggcg
     2881 tcccgcgggc ccggtggctt ctgatcctgg ctccgctcct gtggatgacg ctgtgcggaa
     2941 tcgtgttgta tgtcaaagcg tcctcggcgc acatttccgc gccggagcag atgggccgta
     3001 tgggcggtta tctggccagc tgggccaacg gggcgatgcg ctggaccggg gacgtcgccg
     3061 cccgacacat caccatcggt cccggtgctt atctggcttt cgccgctagc tgttggctcg
     3121 cgctgaaggg tgcgagcggc ctgcgggagt ccgacaccgt accggcggtt ccgaccgact
     3181 gagaatcgtc gcgtgtgtga agacatgcag ccgggccttg tgcgcccggc cgaggtagga
     3241 gcgaatgcac gatgtggtga cagaggagca gctgccggag ctcctgcggc tcgccgaggc
     3301 tgctgctcgc agtgcatcgg agctgatcct ctccggcttt cgcagcccgc ggctcgccgt
     3361 cgaacgtaag ggggacggct cccccgtcac gccattcgat cgcgaggcag agcggaccat
     3421 ccgcgccttc atcgcagcca accagccgcg gccctggccg gtgctcggcg aggagctcgg
     3481 cgatgacacc gggcagtccc gttatcggtg ggtcgtggat ccgatcgatg gcacgctggg
     3541 attcacgcgc gggctgccca atttcggcac gctcctcgca ttcgaggacg cgctcgcagg
     3601 gcgtgcgcta gtcggcgtca tccatctgcc ggcaatgagc gagacctacc gcgcggcgcg
     3661 cggattgggg gcgtggtgtg ccgacgagcg cctgagtgtc gcgccgcccc gggcgctcgc
     3721 ggattgcctg atttcagcgc cgccccaatc gcagttccag ctcgcgggga ctgccgcaag
     3781 tcacgcgaaa ttacgcgcgc aggtacccca tctgcgctgc ttcggagact gctgggcgca
     3841 tgccatggtg gcccgcggac cgatcgatgc gctggcggaa ttcaacctgg cgcgctggga
     3901 cattgcggcc accgaggtcc tggtggaaga aggaggcggg cgggtgttca tccgggatgc
     3961 gcgcgatgtg catggcaaat acgacatcgt gattggcagt ccgcaggcgg ccgcggcggt
     4021 tgccgagctg ttgcaattcg gcgctccggc gcaggtctga gcctcgctca ggtggcgagc
     4081 gctatttcca cgcgcttgtt gtgtttgccc tcattgcgga tccgcaccac gcggatcgcg
     4141 ccgatctccc cgatgttgcg cacgtgcgtg ccaccgcatg gctgcagatc gatgcccggg
     4201 atcctgacca ggcgaattcg cccggcacca cgtggcggct gcacgctcat cgtcttcacc
     4261 agctccggcc gcgcatccag ttcctcgtcc gtgatccaga ctgtctcggt atccagcgcg
     4321 cgggcaatga gtgcgttggt ctcgcgctcg atgcgagcgg catcgagcag cgacagctcg
     4381 atgtcgaaat ccagtcggcc tttatcaggg gagatgttgc cgccggtgac cggtgcagtc
     4441 accacgcagg acatgacatg cagcgccgtg tgcagtctca tcaggctgta gcgccgcgac
     4501 cagtcgagtt ccagcgtgac cggttcaccg acctgcgcgc gcatgggttc gcttgaggag
     4561 ggcacgtgca cgacgctgtt gattgcactg cccttgcggg tgtcgatgac tggaatgcgc
     4621 tcgccgtttt cgcggatcag cgcaccggtg tccccgggct gacctcctcc gagtgggtag
     4681 aaaatagttc ggtcgagctc cagtccacga tcgctctggg caacgatccg cgccgtcgcc
     4741 gtcttcaggt acgcatcttc gcggaacagc agatccgtcg gggtcattcg ggcatgatca
     4801 gtcgagcaga aggttcttga cgcattctag caagcgggaa gtcagggtcg aggtcgaacg
     4861 tttgcgtaga cttgactaca tgaaggctcc tggaaggctc aaatgggtgc tcgtgtcgtt
     4921 actcggatcg agcgtcggtt ttggtgccgc agagcggcca gctctggcgg tgctggacgt
     4981 ggagctcacc ggtgatctgg gcggtccgca attcgaggcc gagcatgccg tgcggctgcg
     5041 catggccagc gagcgactgc ggcaggagct cgcgcgatcg ggtctgtatc ggctggtgga
     5101 cctggcgccc gcccgcggat tgatcctcag gctccagtca cagcaacgct acctgcacga
     5161 ctgcaacggc tgcgatctcg acgtcggacg ccagctcggt gccgaccagg tgctcgtagc
     5221 ctgggtcgat cgggtaagcg ggctcatcct gagcctcacc tacgagatcc acgatgtggc
     5281 ttcgggccgc atcctcgccc ggaagtcctt tgatttccgg ggtgataatg atgctgcgtg
     5341 gacgcacgcc atcggctaca tggtgcgcga tctcgaggcg agctccccat ccgggccgga
     5401 tccggcatcg cactgatcga gattcgggga gcggcccctc gggaatcgcc gggacgatgg
     5461 tggccatggg tcggcattca gcgcgacacc gtgcgcaaca tcatcctcgc cgcctgggtg
     5521 ctgacgctgc ccgtgtgcgt ggccctaggg gccacgctct tcgcgatggg gctgtttttc
     5581 gtcctgcggg tgctcggcgt ccattgaggc gagccggtcc agattagcgc ggatgtgggc
     5641 ggcttctgcc gccgtacgag ccagtgcgat cgcctgggta aaagcaatac gcgcctcggc
     5701 cgtgcggcgg agctccgtca gcagggcacc cttgacgcca aaaaaataga agtacgcggc
     5761 cagcggctcg gcgagcggct cgatcatctt cagcgcggcg cctggaccgt gcaccttgga
     5821 gacggccact gcacgattca gcgtcacgac gggggagggt tgcacttgct cgagcacact
     5881 gtagatcgca tcgatctcag cccagtccgt ctctgtccag cgcagcgcgc gggcgtgcac
     5941 cgcggcgatc tcggcctgca gctggtaagg gcccggctgc tccagccgcc gggccttctg
     6001 taccagtacc agggcctccg cgatcagctc gtgattccaa agccgccggt tctgatcctc
     6061 cagcagcacg atgttggaat tcgcatccag gcgtgccgct gcgcgcgcat gctgtagaag
     6121 gaggagcgca gtgaggccga tgatctcgcc ctggcccgga aacagccgca gcagcaggcg
     6181 cgcaagccgg atggcctcct cacacagagg caaacgcaca tgggcctcac caccgctggc
     6241 ggaatagccc tcgttgaaca gcaggtagat catcgcggcc acggccgtca gtcgctgggc
     6301 acgctcgacc gggccgggcg tctcgaaggc caccgaggca tcggccatgg cgcgtttggc
     6361 acgtgtgatt cgctgcgcca tcgccgcctc gctgaccaga aaggcgctgg cgatctcctt
     6421 gaccgagagt cccgagacga tgcgcagggc gagtgcgatc tgctgggtgg cgggcaaatc
     6481 cggttgacag cagatgaaca gcaggcgcag cacgtcgtcg tgatatgcgg agctgtcgag
     6541 tttttcggcc agcgcagcct cggtatcttc cacgtctgaa accagctcct cgtcggcaag
     6601 cgagtcgagc ttgctgcgac ggcgcatctc atccagcgcg ctgttgcgcc ctacgaagat
     6661 cagccaggcg gccgggtcgc gcggcggtcc attcaccggc cagctcttca gagcccgcag
     6721 acaggcctcc tgaaaggctt cctccgcgac gtccagatcg cggaaatagc gcagcagcgc
     6781 tgccaccgcc tgagggcggg ctgcgctcac cgcggcccct atccatgcgg catccgtcac
     6841 tgcagaccga cgccgggata gaacgtggcg accggtcgaa cctcgagggc ccccgtgtct
     6901 cccacgagat tgcgggctac ggcgatggcc tcctccagtg ttcggcaatc caccatgtaa
     6961 aagcccagaa gttgttcctt cgtctcggcg aaggggccat ccagcacgac cggttctcgg
     7021 ctggctcgca tcgtcatggc ggtcgtggtc ggcatcagcc gtgccacggg tcccaggcgc
     7081 ccctgcgctg ccagatgctg ggccacgacc tctcgctgcg ccagaacggc ggcttccttt
     7141 tcgcggggcc acgcctcgag ctcgctttcg aggttatagc aaagtatgca gtagaacatc
     7201 ggatgggctc cttcgctcta caaggacgag tgagaggggc cggctgagac atgggcttgc
     7261 ataccaatcc gtgcaaggct gcgcgccgcc cgcgggtggc gtgcgcgatc tgctcagaac
     7321 cagccgtagt tgaagctgat gctgatgcgc tcggaagcgg acggattggg cgccacttcg
     7381 tggcgcagcc agctctcgaa gaggaccagg gagccggctt gcgcagggat tacgatccag
     7441 gcgcgattgt ggatgcgggc cccgattcgg ggcggcacgg ccatgaagcg atccagccgc
     7501 gggtcctcga gcttcagacc ggggcagccg gcgggcgtgc gcaggaaata ggtgccactc
     7561 agggtcgaga gggggtgcag gtgcaggcca tgtgccacac gctggggcat gatgttgacc
     7621 cagcaatcgg tcatggtcag cgggcgccgc cttaggtcga actccagcga tcgggcgaac
     7681 aggcggacat gccgccccag atgccgttcg agctcggcaa aggtcggcga gagctgatgc
     7741 agccggctga cggagccgta agaggtgtag ccgccgagat aattccgctt cgaccatttg
     7801 cgtccggccg tatcgtctgc acgcaattgc cggcattcgc gcagcagtcg cggctcgagc
     7861 ctcgctctct cgcggctgcg cagccgtgcc tggtatacca gcgtcggaaa gagtctccgc
     7921 agggtcatcc tggggtccgg gagtccggcg ccgctgccaa gtgctgcact gccggtgcga
     7981 caagcgtccc gggctgcgca tggcgcagcc actcgagaaa gggtcccgcg gcccggtccc
     8041 cctcgggcgg tgcccaggcg gcaaattcgg ccgccgacgc ggcatcgtac gggccttcct
     8101 tgatctcgag aaaggtgctg ccatcggcca tcgcaatgag cgtgtgccag gtctcctggg
     8161 gcgtctcgaa tccgatgctg ggggctccct caccgatcgc gtgacgtgcc gtgaccgtgc
     8221 ccgccgcatc gaatgtcagg atgtcgaagc ggccgcgcag caccatggcg agctcggact
     8281 tcgtgtggtg gcggtgcggc cggaaatagg atgcctggtt cgcggcgatg aagaagcgct
     8341 gcacgcgatc ggccgccgag aggtgaatgt tgtgattcgc gcgcaggcgc gggctttcgg
     8401 ccgcgcgcgc tgcgagctca tcgagcaggg aacgggaaaa cagtttcatg gcaagtggcc
     8461 ctgtacggtg cgggcgccat tgtgaacgat atcgggcggc cacaagccgc gccagcatga
     8521 ggtgacatcg atgagtgcca cgcagaacga gagagctgat cgcttccgag ccctgcacgc
     8581 cgcgggtact ttcgtgatcg ccaatgcgtg ggacggcggc tcggccaagg tgctcgcggg
     8641 gctgggattc aaggcagtag cgacctcgag tgccgcgttc gcctggaccc tgggccggca
     8701 tgacggtcag gtgagccgcg aggaggcgct ggcccatgca cgcctcattg ccgccgccac
     8761 tgcgctgcct gtcgcagccg atctcgagaa gggtttcggc gattcgccgg aggccgtcgc
     8821 ggaaaccatc cgtcttgctg cacgcaccgg cctggtgggg gggtcgatcg aggatgccag
     8881 tggcgatgcg cacgcgccga tctacgaggc cgctctcgcc gcagagagag tggcggccgc
     8941 agtggaagcg gcgcgggcac tccctttttc cttcacgctg accgcccggg ccgagaattt
     9001 cctgtgcgcc aagccggacc tggatgacac gatccggcgg cttcaggcgt tcgagcgcgc
     9061 tggcgccgat gtcctgttcg ctccggggct gccggacctg gccgccgttc gggcggtgtg
     9121 ctcggccgtc tcgcggcccg tcaatttcat ggtgggcatg cgcggacgat cgttcagctt
     9181 tgccgagctg gcggaagccg gcgtgcgccg catcagcctt gcgacttccc tgtaccgtgc
     9241 ggccatggcc gggctcatgt cggcggctcg cgaggtcagg gatcggggaa cattcggatt
     9301 cgtcgatagc cccgtcaaca cgagcgagct ggacgcgttt ctcagggagt agcggataac
     9361 gcgttgcacg gcggaacaag tccctccgcc gtgcgtgtaa aatgttggta cgccgctgaa
     9421 atcggcgtcg gaggtgtgta tgagggcctc agcattccgg cgagtgggaa cgcgcctggt
     9481 cgttcccgtc gcgctgacct cgttcctgtg tggagcctgc ctcgcggccg aggcaggttc
     9541 ccctgaagat gctgcgcagc cggcggtctg gaccacgaaa aagctggatt tcgtctttca
     9601 gggcttcacc gcgaagtact cctgtgatgg gctgaaggac aaggtgacgc gcgcgttgct
     9661 gcagctcggc gcccagaaga aggatctgaa ggtgcgcgag atgggctgtg tgaacgggtt
     9721 cggcagaccg gacccgtttc ccggcgtcag tatcaagatg agcgttctgc agcccgccag
     9781 cgggagcacc gacagcagtc agcccccggt cgcggcgcac tggaaacccg tcaagatcaa
     9841 tctccgcgac atcaacgact atccgaacga ctcgggagag tgcgagctct acgagcagat
     9901 ccgggagaag gtggtgccgc tcttcagtac gcgcaacgtg gatctgagtg gcatctgcgt
     9961 gccccaccag gagtcagccg cggggccgac tctgaagctc gaagtgctgc aggcggacgc
    10021 caaggacact cggtcggcct cggccaagtg atggtcaggc ggccccagcc gcctgagctc
    10081 gcggtgccgg atcgtgtttc tcaacgtccg gcaagagacc cgccaggacg ccgatcagcg
    10141 gcaggaaggc gcagatctgg taaatggtgg taatgcccca cagatcggcc agcttgccga
    10201 gcacggccgc gccgatgccg cccatcccga aggcaaatcc gaaaaacaag cccgccacgg
    10261 tgcccgttcg accgggcatc agctcctggg cgtataccac gatggcggga aaggcggagg
    10321 cgaggatcaa tccgatcggt acgctgagca ccccggtcca gaagagattg gcgtgcggca
    10381 gcagcagact gaaaggcaac accccgagga tcgagaccca gatgatgtac cgtcgtccga
    10441 cacggtcccc gaggggaccg ccgatcatcg tgccgactgc aactgcgccc aggaagacga
    10501 acagatgcag ctgtgagctc tggacggaga cgtgaaagcg gttgatcaga tagaaggtgt
    10561 agtagctcgt caggctcgcc aaataaaagt acttcgagaa gatcagcgcc agcagcacgg
    10621 cgatggcacc gcgcatgcgt cgccggtcga ttgtcatggg cttatcgatc ggtgatgcgc
    10681 gtggcttgag cgacgcaagc ccgtgcgctt tgtaccagtg tccaacctgg aagagcacga
    10741 acattgccag aagtgccgcg cctgagaacc atgcaacgct cgactggccg cggggcaata
    10801 cgatgaaggc agcgagcagg ggaccggtgg ccgagcccac attgccgcca acctggaaga
    10861 ccgactgcgc caagccgtgc tgcccgccgg aggccatgcg cgccacgcgc gaggactccg
    10921 gatggaacac cgaggagccc gtgccgatga gcgccgccgc gaacagcagg agtgcgtagc
    10981 tggaggcgaa cgcgagcagc accagtccga ccagcgtgaa ggccattccc atggcgagcg
    11041 agtaggggcg cggcgagcgg tcggtgtagg agccgatcac cggctgcaaa agcgaggccg
    11101 tgatctggaa ggtcagggtc agaatcccga tctcgccgaa gctcagcgca tacgagctct
    11161 tcagcatcgg atacagcgct ggcagcagcg actgcatcat gtcattcagc aggtgacaga
    11221 acccgatcgc cgcaagaatg ctgaacgtgg tccggctggc ggaagcggcc ttggacaatg
    11281 tggcagtggc ttcagtcaag gagcccagca tgccgcaacc cgttcccgat gactatctcc
    11341 cggaatgggg gcctgcgatg caggccgctc agcgggcgcg cccgcgcgaa cccccggcta
    11401 agccctgctg gacgtagccg gggtgtcccc gtttggcggc gcgatggccg gccgctagaa
    11461 gcgttgaccg aatttcaacc cgatcattcg cggcacgttc ggcgtgttgt agattgcgac
    11521 cgttccgcat tgaagcacac cgcaactggc cgtgcgatcc aggattgcgc gcttgtcgaa
    11581 cgcgttgctg acaaacagcg tcgccgtcca gctgttcctc gccagctcgg cggagagatc
    11641 ggtgaccgca taggagggca tctccccaaa tgcatcccga tccacgatac gcaggtccgg
    11701 ccagcgcgcg ccgacgtacg tctccgacgc ctgcagagtc gcttggaact cattcggcaa
    11761 cgggatggta tatcggcccg tgaggttggc cttgaacttc ggggtcaccg gtagctgcgt
    11821 acctgagggg gcatagcctt catagcccgg cacgaggcag tccgccaatg tgccaccgtt
    11881 tccgatggca tcgcagtacg tttgagtcag cttggcattg agataggctg cgctggccga
    11941 caacgtgaag gggttcgcga cgcgccactc catctccgat tcgactccct tgatctcggc
    12001 gttgccggcg ttattgatga tcgtcagcgc atttggcccg aggaaggcga actgaaagtc
    12061 cttccacttc tcgtcgaaga tcgcgccgtt ccagcgcagg ctgttgtcca accaggtggt
    12121 cttccagccg atctcgtaat tcgtgaggaa gtccggcttg tagggtggca gcttgccgcc
    12181 gccgttgcgg tttacaccgc cgggccggaa ccccttcgac caggttgcgt acaccatgtg
    12241 atccggatcg atcttgtagg tcagattgat cttgggtgag ttgcccgtgt cgttgctgcg
    12301 gccattgaga tcctgacaga ccgcgccgtg gaacggcacg aacggcgtga agcaggtcgc
    12361 gatgccttca tgactgctgt aggtggagtt gaagccgtag aacccctgca gggtgttgtc
    12421 gaagcgccag tggcgcaggc ccgcgttgag agtcaaggcg ctcgtgatgt cccaggagag
    12481 ctcgccgaac accgcggcat cccgatccac acgctcctcg tcggtgagcc atatcgtgcc
    12541 cggccagcca ggtaccgata gattgaatgg gcccgtagag gcgagcggat cgttcatggc
    12601 agggccaccc ggtatggtgt agtcctgcaa gatctcgtgc acctgcctct ggaggaatag
    12661 gcctgccgtt acccggacag ggtattcctt cggcgaggtc acgcgcagct cgtggctcaa
    12721 cttggtatag tgatcgcgcc ccacgatgta ttgcgccgga ttgatgagtt tgcccgcatt
    12781 gtcgcggaag tacgtggcgt agctgtcgaa ggtataagcg gccgtgtagg ccagcgaata
    12841 gtcagtgtaa tcctcgttct cgtgcgtgtt gcgggtcagg tatgcacctg cgtacaccag
    12901 atcgaagttg ttgatcttgc cctcgatcgt cagcgccgac tgtacccatg agtcatggat
    12961 ctcctcgggg aagtacttct gcacttccag gtcaccgagt ttggggttgt aggcgaagaa
    13021 tccgtgcgtg tcactcgact gacccatgac ctgcggcgtg acagtccagt tgtccgtcag
    13081 atcgaccttg agcgccgcgc gaccaccctt ggtctcgaca tcgttgtaat ccttctttac
    13141 ctgcgcggca ttgttctgcg tgaaagtcgg cggcccgcct tgcggaacat aggtcacggt
    13201 cccgggtacg ttgtcgatga atcccgcatc cttctcatcc caacccacca ggcgcacggc
    13261 cgcgatggag gagatgggca
//
LOCUS       DASRHN010000375         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2283578, whole genome shotgun sequence.
ACCESSION   DASRHN010000375 DASRHN010000000
VERSION     DASRHN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283578"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1295)
                     /locus_tag="VFB37_15125"
     CDS             complement(288..1295)
                     /locus_tag="VFB37_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO23833.1"
                     /translation="MSAIAISRRQALLGTAAATGAAALGGAPLPAFGKAPFAKDQVPY
                     FYRFMHGKMQATVVSDGILPLGEPSASFLGTSKEEVAKLLTDNFLSPTNVVLEQNALV
                     INTGDKLVLFDTGMGSSTMFGPTTGKLLASLKAAGIDPKDIDAVVATHAHCDHVWGIM
                     ADDGGRHFPNAQIYISQADFDFWTDEAKLNVKDPAYMKPFVEGARKNLLPNRDRMIYV
                     KDGQEFLPGIQAMAAPGHTVGHTIYMVTSDGKTLAVIGDLTHHQVLLVEKPRIEFAYD
                     TDSKQSANTRVRVLDMLAANRIPLIAYHFPWPGVGHVAKHGDGFRYFPAPLQMVLEQK
                     A"
     gene            1547..>2008
                     /locus_tag="VFB37_15130"
     CDS             1547..>2008
                     /locus_tag="VFB37_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23834.1"
                     /translation="MRKRRVAIACQGGGSHAAFAAGVLSGLLSGEDGDRFDQDYRLVA
                     LSGTSGGAVCASLAWSGLHSKGHDDKEGRAEASRRLLGFWRDLEAWWPLDQWINACAL
                     AVARLPFTFEVSPYLCIPFAEARLRQLLERHLRLEELRRQAQPRSCPKLLVG"
BASE COUNT          327 a          622 c          695 g          364 t
ORIGIN      
        1 cagaaaagcg gcaaggactg tgtgccgacg catgtacgca tttcccccgg tggctttcga
       61 cagcactcgg tcctgcatag actcatcaaa cggccggaat gcggcccgat ttggccgcgc
      121 ttgccacgat tgcggcagcc ggcacacgat gcatgtgcaa gcacagggcg cagctccggg
      181 gccactccta cagcacaatc gttgccggag agtgagcggt cgaaaggcaa attggaaaga
      241 ggcccgccgg ggcgggcctc tcatgctgca aagccgggct tctgcgctca ggccttctgc
      301 tccagcacca tctgcagggg tgccgggaag tagcggaagc catcgccgtg cttggccacg
      361 tgcccgacgc cgggccaggg gaagtgatag gcaatgagcg ggatgcggtt cgcggccagc
      421 atgtccagga cgcgcacgcg cgtgttggcc gactgcttgg agtcggtgtc gtaggcgaac
      481 tcgatcctcg gcttctcgac cagcagcacc tggtgatgcg tcaggtcgcc gatgaccgcc
      541 agcgtcttgc cgtcggaggt gaccatgtag atcgtgtgcc caacggtgtg gcccggagcc
      601 gccatggcct ggatgccggg caggaactcc tggccgtcct tgacgtaaat catccgatcg
      661 cgattgggca gcaggttctt gcgcgcccct tccacgaacg gcttcatgta ggccgggtcc
      721 ttgacgttga gcttcgcctc gtcggtccag aagtcgaagt cggcctgcga gatgtagatc
      781 tgggcattgg ggaaatgccg gccaccgtcg tcggccatga tgccccagac gtggtcgcag
      841 tgcgcgtggg tggccaccac ggcatcgatg tccttgggat cgataccggc agccttgagg
      901 ctggcgagca gcttgccggt ggtggggccg aacatcgtcg aggagcccat tccggtgtcg
      961 aacagcacca gcttgtcgcc ggtgttgatg acgagcgcgt tctgctcgag caccacgttc
     1021 gtgggcgaga ggaagttatc ggtcagcagc ttggccacct cctccttgga ggtgccgagg
     1081 aagctggcgg acggctcgcc gagcggcagg atgccgtccg agaccacggt ggcctgcatc
     1141 ttgccgtgca tgaaccgata gaagtagggc acctggtcct tggcgaacgg ggccttgccg
     1201 aaggcaggca gcggcgcccc gccgagtgcg gctgctccgg tggcggctgc ggtgccgagc
     1261 agtgcctgac ggcgcgagat ggcaattgcg ctcattgtgt ctcctccgtt gttgctgttg
     1321 ctcgagtatt tcgttggaag gccagcttac gcccggttgg gggcgacttg agggcatgcc
     1381 ggctgcgggc atcgagcctg ctgaaaccgt gtcggcctga ggaccaacgc gttcccgaca
     1441 caccgacaaa cgccacaagc gttcaaacgc cacaagcgtt gccgctcgat tgcccggggt
     1501 cccgaaacgc cgcacactgg gtgttctgca aagcgggagc cgggccatga gaaagcgcag
     1561 ggtcgccatt gcctgtcagg gaggcggcag ccatgccgcc tttgccgccg gagtgctcag
     1621 cggtctgctg tcgggtgagg acggcgaccg cttcgatcag gactatcggt tggtcgctct
     1681 cagcggcacg tccggcggag ccgtctgcgc ctccctggcg tggtcgggcc tgcactcgaa
     1741 aggtcacgac gacaaggagg gcagggcgga ggccagccgc cggctgctcg gcttctggcg
     1801 cgatctggag gcgtggtggc cgctcgatca gtggatcaac gcctgcgcgc tcgccgtcgc
     1861 ccgccttccc ttcacgttcg aggtcagccc ctacctgtgc atcccgtttg ccgaagcgag
     1921 gctgcgccag ctgctcgagc gccatcttcg tctggaggag ctgcgtcggc aggcgcagcc
     1981 gcgttcatgc ccgaagctgc tcgtcggt
//
LOCUS       DASRHN010000376         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_138103, whole genome shotgun sequence.
ACCESSION   DASRHN010000376 DASRHN010000000
VERSION     DASRHN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138103"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFB37_15135"
     CDS             <1..238
                     /locus_tag="VFB37_15135"
                     /inference="COORDINATES: protein motif:HMM:NF018630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZO23835.1"
                     /translation="QAGAAPKVERVHHSGGPEDGQRPPSPCVNICELDDAGFCIGCLR
                     TGDEIGLWSAMSPAQQWRLIADLRVRKLRAAGSV"
     gene            272..595
                     /gene="grxD"
                     /locus_tag="VFB37_15140"
     CDS             272..595
                     /gene="grxD"
                     /locus_tag="VFB37_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZO23836.1"
                     /translation="MDVVERIKTELASSPVVLFMKGTPDFPQCGFSAQTVAALRALGA
                     DFSHVNIFEDPELREALKRFSNWPTYPQLYVRGELVGGCDIALEMYRSGELKQLLADA
                     GAVAA"
     gene            complement(620..1387)
                     /gene="rnt"
                     /locus_tag="VFB37_15145"
     CDS             complement(620..1387)
                     /gene="rnt"
                     /locus_tag="VFB37_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HZO23837.1"
                     /translation="MTATPAAQGDAPATPAAGPAASPPPSAAANLMARRFRGFLPVVI
                     DVECGGFYAETDALLEIAAVLIDITPEGTLGRGATHHFHVKPFEGARLDPAALSVTGI
                     DPHHPLRPAIPEKDALGRVFREVRHAVQAHACRRAILVGHNAAFDLGFLNAAVKRADV
                     KRNPFHPFSCFDTATLAGAALGQTVLAKALAVAGLEWDPSSAHSAAYDAERSAELFCL
                     ICNRLRDSFRDAHERACLLGWLSTPPDEALVEDRPLE"
     gene            complement(1435..>2067)
                     /locus_tag="VFB37_15150"
     CDS             complement(1435..>2067)
                     /locus_tag="VFB37_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23838.1"
                     /translation="EVTDPSVDIFDRERRFIESVLAPLLERLPRLRVVFEHITTREAV
                     GFVTGSRARIAATITPQHLAMNRNALFNGGIRPHHFCLPVLKSEPDRLALLEAVAAGH
                     PRFFLGTDSAPHARHSKENACGCAGIFSAHAAIELYAEIFEAAGALARLEGFASEFGP
                     QFYGLPVNEDRITLVQEPWHVPSRYPFGADELIPLRSGERLSWRLAPASA"
BASE COUNT          364 a          640 c          720 g          343 t
ORIGIN      
        1 ccaagctgga gcggcaccta aggtggaacg agtgcatcac agtggcgggc ccgaggatgg
       61 ccagcggccc ccctctccct gcgtgaatat ctgcgagctg gatgatgcgg gcttctgcat
      121 cggctgcctg cgcaccgggg atgagattgg tctctggagc gccatgagtc cggcgcaaca
      181 gtggcggttg atagcggacc tgcgcgtgcg gaaactgcgc gctgcgggct cggtctgagt
      241 aagctggcac ggcgccagcg gaggtatctg gatggatgtc gtcgagagaa tcaagacgga
      301 gctcgcctcg agcccggtcg tcctgttcat gaagggcacc cccgatttcc cgcagtgcgg
      361 cttttcagcg cagacggtag cggcgctgcg cgccctggga gcggacttca gtcatgtcaa
      421 tatcttcgaa gatcccgagc tgcgcgaggc gctgaagcga ttctcgaact ggcccaccta
      481 tccgcagctc tatgtgcggg gcgagctggt cggcggctgt gacattgccc tggagatgta
      541 tcgcagtggc gagctgaagc agctgctcgc ggacgcgggc gccgtcgccg cctagttcat
      601 cggaaaagca ggcgcgggct cattccagcg gccgatcttc gacgagggcc tcatcgggtg
      661 gcgtgctgag ccatccgagc aggcacgcgc gctcgtgcgc gtcacggaaa ctgtcgcgca
      721 gccgattgca gatgagacag aacagctccg cgctgcgctc ggcgtcgtac gccgcggagt
      781 gcgcgctgct cggatcccac tccagacctg ccacggcgag cgctttcgcg aggaccgtct
      841 ggccgagagc cgcgccggcg agcgttgccg tgtcgaagca tgagaacggg tggaagggat
      901 tgcgttttac atccgcacgc ttcaccgcgg cgttgagaaa tcccaggtcg aaggccgcgt
      961 tgtgacccac caggatggca cgccggcaag cgtgtgcctg cacggcgtgc cggacctctc
     1021 gaaatactct ccccagtgca tccttctccg gaatagccgg ccgcagcggg tgatgcggat
     1081 cgatgccggt gacggacagc gcggcaggat cgagccgcgc gccctcgaat ggcttgacat
     1141 ggaagtgatg agtcgcaccg cgcccgagtg tgccctcggg tgtgatgtct atcagcacgg
     1201 cagcaatctc cagcagcgca tcggtctcgg cgtagaatcc gccgcattcc acgtcaatga
     1261 ccacgggcag gaatccccgg aagcgccgcg ccatcaggtt cgcggccgcg gaaggcggtg
     1321 gcgaggcggc gggacccgcg gcaggcgtgg cgggcgcatc gccctgagct gctggcgttg
     1381 cggtcactgc atcccggaca ctcaactatg cgtacctcat tgcagccggg gtaactacgc
     1441 ggacgcgggt gcgagtcgcc aggaaaggcg ctcgccgctg cgcagcggaa tgagctcgtc
     1501 cgcgccgaaa ggatagcggg aagggacgtg ccacggttcc tgcacgagcg tgatgcgatc
     1561 ctcgttcacc ggcaagccgt agaactgtgg cccgaactcc gaggcgaatc cctccagcct
     1621 ggcgagcgcg ccggcggcct cgaagatctc cgcgtacagc tcgatggctg catgggctga
     1681 gaatattccc gcgcagccac aggcattttc ctttgagtgt ctggcgtgcg gcgcgctgtc
     1741 ggtgcccagg aaaaatcggg ggtgtccggc tgcgaccgcc tcgagcaggg caaggcgatc
     1801 gggctcgctc ttcagcaccg gcaggcagaa atggtgtggc cggatgccgc cgttgaaaag
     1861 cgcattgcga ttcatggcga ggtgctgtgg agtgatggtc gcagcgatgc gggcgcgcga
     1921 gccagtcacg aatccaacgg cctcgcgtgt cgtgatgtgc tcgaacacga cgcgcaggcg
     1981 cggcaaccgc tccagcagcg gcgcgagcac cgactcgatg aaacggcgct cgcggtcgaa
     2041 aatgtccaca ctcggatcgg tgacctc
//
LOCUS       DASRHN010000377        15521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2439508, whole genome shotgun sequence.
ACCESSION   DASRHN010000377 DASRHN010000000
VERSION     DASRHN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2439508"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFB37_15155"
     CDS             <1..798
                     /locus_tag="VFB37_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO23839.1"
                     /translation="PEEFETRQVFYKSKDGTRIPLYIVARKGTKLDGNNPTLLYAYGG
                     FNISLEPQFSPAVAGWVAMGGVYAMANLRGGGEYGRTWHEAGMKTHKQNVFDDYIAAA
                     EYLIAQHWTQPKHLAINGRSNGGLLIGAVEEQRPELFAAAVPQVGVMDMLRFRDFTVG
                     KGWESDFGSVDNPAEFRAILAYSPLQNVKPGVNYPPTLVTTGDHDDRVYPAHSFKFTA
                     AMQHADPHGNPILIRIDTRAGHGSGKPTTKLIEETADIYAFIWNIIK"
     gene            816..1154
                     /locus_tag="VFB37_15160"
     CDS             816..1154
                     /locus_tag="VFB37_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZO23840.1"
                     /translation="MQAPPILIVGAGPTGLVLALWLTRMGVPVRLIDKLPAPSPTSRA
                     LAVQARTLELYRQADLTTHVLEHGHRVPALNFWAAGQVVARVPFEAIGTGLTPYPFAH
                     IFRRTSTSGS"
     gene            1151..2335
                     /locus_tag="VFB37_15165"
     CDS             1151..2335
                     /locus_tag="VFB37_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZO23841.1"
                     /translation="MTERLAGQGVSVERGSELVGLTQDESGITALIRRPDASETDCRA
                     LYVAGCDGARSLVRTAIGTGFPGGTYRQVFYVADVEAEGPPINGELHVDLDEADFLAV
                     FPLAGEHRARLVGTVRDERADRADTLRFEDVSTSAIRHLKVEVRKVNWFSTYHVHHRV
                     TEHFREGRAFLLGDAAHIHSPAGGQGMNTGIGDAINLAWKLASVLGGRAPAALLDSYE
                     RERIGFARRLVATTDRLFSFATAEGRIADIMRTRVTPFLFPKLVSFDRVREFLFRTVS
                     QIAIQYRDSPLSAGVAGQVHGGDRLPWVQLEGRDNFDPLAAMTWQVHVYGTPRADLTQ
                     SCADRRLPLHVFPWSTAAEHGGLARDALYLLRPDSYVALADACADPGAIERYCAERGI
                     RV"
     gene            complement(2305..3486)
                     /gene="pqqE"
                     /locus_tag="VFB37_15170"
     CDS             complement(2305..3486)
                     /gene="pqqE"
                     /locus_tag="VFB37_15170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HZO23842.1"
                     /translation="MSLEPTSPLGAQASTASLGPPLWLLLELTYRCPLHCVFCYNPID
                     FSRTALELSTAEWIRVLHEARALGSVQLGLSGGEPLSRDDLETIVAEAHTLGYYINLI
                     TSGIGLTESRIEALKKAGLDHIQLSFQDSTREVNDFLSSTRTFELKSKVAALIKRYEY
                     PMVLNVVLHRLNIDHIEQILDMAQEMKADYVELANTQYYGWAWHNRERLIPSRAQLEH
                     AEAVTQRFREHHRRMKIFFVVPDYFERRPKACMNGLGSVFLTVSPDGTALPCHAARML
                     PGLTFPNVREAAVEWIWRESPAFNHFRGDAWMKEPCRSCPEKIRDFGGCRCQAYLLTG
                     DAANTDPVCDLSPHHHLVTQMVERASVPGLAVEKPLVFRDRRNSQTLSEDYTRIPRSA
                     Q"
     gene            complement(3483..3791)
                     /gene="pqqD"
                     /locus_tag="VFB37_15175"
     CDS             complement(3483..3791)
                     /gene="pqqD"
                     /locus_tag="VFB37_15175"
                     /inference="COORDINATES: protein motif:HMM:NF002535.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HZO23843.1"
                     /translation="MSAAGGGAEPSSPAGLQAKPTLGHGLRLQWEPAQNAHVLLYPEG
                     MVKLNGSAGEILRRCNGERTVAEIVAELEAAFSTDGLTADVTAFVAVALQKHWLELRE
                     "
     gene            complement(3788..4486)
                     /gene="pqqC"
                     /locus_tag="VFB37_15180"
     CDS             complement(3788..4486)
                     /gene="pqqC"
                     /locus_tag="VFB37_15180"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HZO23844.1"
                     /translation="MTATAWSRTEFEQQLREKGRAYHIHHPFNVMLNSGAASPEQIRG
                     WVANRFYYQISIPIKDAAILANCPDRTVRRSWVQRILDHDGYGEDRGGIDSWLRLAEA
                     VGLSRTHVESLADVLPGVRFAVDAYVNFARHSAWQEAVCASLTELFAPEIHKQRLAGW
                     PQHYPWIDQSGLHYFQSRVSLARRDVEFGLAVTLDHFVTRAAQERALQILQFKLDVLW
                     QMNDAMQQRYGVRS"
     gene            complement(4483..5403)
                     /gene="pqqB"
                     /locus_tag="VFB37_15185"
     CDS             complement(4483..5403)
                     /gene="pqqB"
                     /locus_tag="VFB37_15185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HZO23845.1"
                     /translation="MKIRVLGAAAGGGFPQWNCNCRNCAGLRAGTIRARARTQSSIAV
                     CGTRESSWALVNASPDIRAQLQANPVLQAGRTLRDSAIAGIVLVDAQVDHTTGLFMLR
                     ESSRPWRIWCTDSAYTDLTVGNPIFKVLGHFCGTERRRIDLHGERFTVEGVAGVQWQA
                     LAIEGKPAPYSPNRENPVPGDNLALVITDELTGGSAVYAPGLGSMDERLWQHMQSAAC
                     VLLDGTFWTDDEMIRLGASRKRARQIGHLPQSGPGGMLEWLDKLPSGTRRILIHVNNT
                     NPILDEDSREAAELARRRVEVAWDGMELSL"
     gene            complement(5460..5534)
                     /gene="pqqA"
                     /locus_tag="VFB37_15190"
     CDS             complement(5460..5534)
                     /gene="pqqA"
                     /locus_tag="VFB37_15190"
                     /inference="COORDINATES: protein motif:HMM:NF001400.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HZO23846.1"
                     /translation="MKWETPQATEMRFGMEITMYIANR"
     gene            5829..7175
                     /locus_tag="VFB37_15195"
     CDS             5829..7175
                     /locus_tag="VFB37_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO23847.1"
                     /translation="MKLRTRLNLVVTGLTAMFVLVLIAAEIEDTRSSVREEIEAANRV
                     GSQLLGRLVAIYSREGGPQVVLEFLQQLGRVRANEVSLWAPGGRVLYRSPPATYKAGR
                     EAPRWFEHLLAPQAARHTFYLPDGVQLVVEANPSRAILDAWDDLTRLFEIGTIMLVLV
                     SGLAFWLVGRALAPFPVIAMGLARIEAGDLAFRLPPLAGAEAHAIGAAFNQMAQAVQD
                     KVRVERQAREAESRLEERRELARLVEQRIEEERRLIAHELHDEFGQSVTAIRSLAMAI
                     STQSKDPTMTDAARLISQEASRLYDAMHGLIPRLTPLSLDTLGLADTLENLVRDWQRR
                     YPDVALCARHALAAELGPSVTLTIYRVAQEGLINALRHAHATRVEIDLHAREDRIVLT
                     ITDDGVGLPADWARPGHFGLRGLADRVENLGGDFKVGNCEPHGVRLTAEIPLVAGA"
     gene            7172..7807
                     /locus_tag="VFB37_15200"
     CDS             7172..7807
                     /locus_tag="VFB37_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO23848.1"
                     /translation="MIRVLLVDDHAVVRTGFRLLLQSQSDISVAAEAHSGEAACQIYA
                     ELTPDVVVLDLGMPGMGGLEALKRIRARHAAARVLALSAHDDPMHARRALREGALGFL
                     SKRSAPEALIEAIASVAAGRRYIDPTLAQKLALAEIEGAARSPIEHLSEREFEVFIRL
                     AGGATVQKIAEDLKLSASTIGTHLYNIKQKLGVVNQAELTLLAIRHGLIEA"
     gene            7879..9300
                     /locus_tag="VFB37_15205"
     CDS             7879..9300
                     /locus_tag="VFB37_15205"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO23849.1"
                     /translation="MSRGFFAAIGAALLWTGPCGAETDQASDALARDIFKQLIEIDTT
                     ESSGNVTKASEAMAQRLNAAGFAASEMQILGPDDRKKNLVVRLKGTGKHKPILLIGHL
                     DVVEARRSDWSTDPFKFVEKNGYIYGRGTQDMKNGDAIMLATLIRLKKEGFRPSRDII
                     LALTADEEGGSSNGIEWLLRHHRELVDAQFVLNHDSNSVLSEHGKPVYFEMDGTEKVY
                     ADYQLTAVNRGGHSSVPIPDNAIYELSAALMRLSKYEFPFELNSVTRGYYERMSSIET
                     GQRAADMRAILKNPPDTAASERLSRDPVDHSMMHTTCVATRLEGGHANNALPQRAQAT
                     VNCRILPGHSPEEVRGILIKVLADPKLRVQYIDESGQVQDTAPGRRGYAPPPLDPQVM
                     QPLDTVVDQMWPGLKVVPAMSTGSSDGLFTAAAGLPTYVISGVAVDRDNDRAHGQDEN
                     VGVQSFYHGNEFFYRYIKALTAD"
     gene            9446..9880
                     /locus_tag="VFB37_15210"
     CDS             9446..9880
                     /locus_tag="VFB37_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23850.1"
                     /translation="MRGEWPEHIENALQHLWQHAARSIPEWLPMRYVHWLPTVYEIAA
                     GFHAARSGRTNVYLVLLDYSDHGSEPHGIYVGMSHYAPAQRFDQHKAGIRAAGCVLKR
                     GLEVLTGPTLHLQFIARKEAARIESELAIALAEAGLRVEGGH"
     gene            9950..11392
                     /locus_tag="VFB37_15215"
     CDS             9950..11392
                     /locus_tag="VFB37_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZO23851.1"
                     /translation="MSGVPNRADMAEMAPEGGLAGDDRARLVAELGNRVGSLGVELAD
                     VAGNLEEVTNRVANQAEQFKELQHAAQAMVAGNREIDRAAKETQLAASTAGTEIAESR
                     TLIGTAAQHIGDLTAAVGHIEERLGSFSALLRQIGGVATAIERIAKQTRLLSLNAGIE
                     AARAGEAGRGFAVVAGEVKSLAEETRTATDRIGEIVRALGSQIEGLISESGAATRHAG
                     HAGSGTEKVKGVITRAHEAFNIVGREIDAIAQSAADNLEACDTTLAELDELAVGVDLS
                     SSNLRRADQRIERLLTLSETLIEFIAQSGVETADTPIIRLGIQTAARIGAEFEAAIAR
                     GEITAARLFDERYREIPGTNPKQFLTDYTAFTDRLLPPIQDPLQRSDPRIVFCVAWAK
                     GGYLPTHNPEYSKPQGQDPAWNAANCRNRRLFSDRAVQKVATNTKPFLLQTYRRDMGA
                     GKFVLMKDLSAPIFVHGRHWGAFRIGFRQS"
     gene            complement(11459..14704)
                     /locus_tag="VFB37_15220"
     CDS             complement(11459..14704)
                     /locus_tag="VFB37_15220"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZO23852.1"
                     /translation="MNELRVRRLLLWVLLPVVLLVGLYAGLGFLAVPRLLRSGVQDYV
                     SEHYQRRVVLGVIRFNPFTLRLDVSDFTLPDSDGQPMIAFRHLMVDATIASLWRLGPD
                     FEAIVLEQPYARVLIRPDGKLNLQDLASGQKPSSKPSSGPGRLFINHFSVRGGNVAFE
                     DRAHPSVFRAEIKPINFELRDFSTVGNEAGSYSLSATSEAGEQFKWAGSLAASPLSSH
                     GQFEVANLQAHTLWNYVRDSVHFELPSGAISLHGDYNFTASTSPVGLAVNVHDLTVSD
                     LALRPKGGTEDYVKLGKVEIAETRADLASRSVTVGNVHLAGGTIRTWLDSSGNLNILE
                     LSSGPSGAQSSAPAPTAPSQSSPAATSPTAPTAPTAPTAPAASGSASGTWQVSVPDVR
                     VDGLHVSAQDRQVTPAASLELEGLSLHVTGLRLPRTSPLTVTLSTHVNHEGKLDAKAD
                     LSPDSAIKLQGEVGQLDLTALQPYIAQHTAMTLRSGLLSAKLSVDRGADGNLSAAGDA
                     ELSRVRTVDNVLKQDFIKFERLQLSGIDFKSSPRSLRVHSILARAPYARVIIESDRTV
                     NVSKVLLLKSASKPDGGEPPAESTGAAPISAEATASVMEPQPPPSNSQSAANAATESS
                     SEATPAPVNPKATSSDRRHRHPPKTASTSSAAQSQLALAIDSVRIENGSANYSDYWIQ
                     PHFAVGIQTLQGSITGLSSDPRARAKVELQGKVDRYAPVHIWGTVNPLAATAYSDLNM
                     SFKGMELSTVTPYSGHFAGYKIEKGKLSVDISYKIENRQLTAQHRFVINQLELGDRVE
                     SPDAVKLPIKIAVALLKDRNGVIDLNLPVTGSLDDPQFSLGPLIWKAVLGLLTKVATA
                     PFAALGHLFGGGGAEMKYVDFSPGSASLDPHERDKLTTLAKALKEKTQLGLDVPMTFS
                     PELDRPGLALAQLDRRLLALVQQGTGSGRHKSPASPGDDKPDPNASPPADPALSDPGA
                     RFHLLVALYHQELGKDAALPDSAQAIVAAEKKKAPPPDFGPANLELQSALLPRMTVSD
                     NQLEALGRRRAQAIQDTLLSGGDVDPSRVFIIGNSSKNDSKDKVRVELSLK"
     gene            complement(14715..15173)
                     /locus_tag="VFB37_15225"
     CDS             complement(14715..15173)
                     /locus_tag="VFB37_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO23853.1"
                     /translation="MSAAPIARPALRLAAFVPFRLNRLAAAVSDHLAEIYRERFGLEI
                     PEWRVMATVGPVRGCTAQHIASSTRMHKTRVSRAIANLVARGLIERASSADDRREMEL
                     RLSRSGRRLYQALVPLALDRERELLACMSPAQRRAFTRGLAVLELFLGLA"
     gene            15228..>15521
                     /locus_tag="VFB37_15230"
     CDS             15228..>15521
                     /locus_tag="VFB37_15230"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HZO23854.1"
                     /translation="MSASPKPTAGADPGGLQFQQGFGNHFCTEALPQALPQGRNSPQR
                     CPYGLYAEQFSGTAFTAPRHANRRSWLYRIRPAALHGPFHRIEDGSLTSDFTEL"
BASE COUNT         2733 a         4854 c         5194 g         2740 t
ORIGIN      
        1 ccagaggagt tcgagacccg ccaggtgttc tacaagagca aggacggcac gcggataccg
       61 ctctacatcg tcgcccgcaa gggaacgaag ctcgatggca acaatccgac gctgctgtat
      121 gcgtacggag gtttcaacat ctcgctcgag ccgcagttct ccccggcggt ggccggctgg
      181 gtggcgatgg gtggcgtgta cgcgatggcg aacctgcgcg gcggaggaga atacggtcgt
      241 acgtggcacg aagcgggcat gaagacccac aagcagaacg tgttcgatga ttacatcgcc
      301 gcagccgagt acctgatcgc gcagcactgg acccaaccga agcacctcgc gatcaacggc
      361 cgctccaacg gcggcctgct gatcggggcg gtggaggagc agcgtcccga gctgttcgct
      421 gcggcggtac cgcaggtcgg ggtcatggac atgctgcgtt tccgcgactt caccgtgggc
      481 aaaggatggg aatcggattt tggctcggtc gataacccgg cggaattccg ggcgatcctc
      541 gcctattcgc cgctccagaa cgtcaagccg ggcgtgaatt atccgccgac gctggtgacc
      601 actggagatc atgatgaccg ggtctatccg gcgcacagct tcaagttcac cgcggccatg
      661 cagcacgccg atccgcatgg caatccgatt ctgatccgca tcgacacccg cgccggccat
      721 ggctcgggca agccgactac caagctgata gaggagaccg cggacattta cgccttcatc
      781 tggaacatca tcaaatagag tgcgcgccgg agcgtatgca agcgccgcca attctcattg
      841 taggtgcggg cccgaccggt ctcgtcctgg cattgtggct gacgcggatg ggtgtgccgg
      901 ttcgactcat cgacaagctg cccgcaccct caccgacctc gcgcgcgctg gccgttcagg
      961 cccggacact cgagctatat cggcaggccg atctgaccac gcacgtcctc gagcatggac
     1021 atcgggtgcc ggcactgaat ttctgggctg ccggccaggt cgtcgcccgt gtgcccttcg
     1081 aggccatcgg gaccggtctg actccctatc cgttcgccca tatttttcgc aggacgagca
     1141 cgagcggctc ctgaccgagc ggctcgccgg gcagggggtc tcagtggagc ggggcagcga
     1201 gctcgtggga ctcacgcagg acgaaagcgg catcacggct cttatccgcc gtccggacgc
     1261 cagcgagact gactgccggg cgctctatgt cgccggatgc gatggtgcgc gctcgctggt
     1321 ccgtacagcg atcgggacag gctttccggg gggtacctac cgtcaggtct tctatgtcgc
     1381 ggacgtcgaa gccgaaggtc cgccgatcaa cggcgagctg cacgtggacc tggatgaggc
     1441 agacttcctg gccgtgttcc cgctcgccgg ggagcatcgc gcgcggctgg tcgggacggt
     1501 ccgcgacgaa cgcgcagatc gtgccgatac tctgcggttc gaggatgtga gcaccagtgc
     1561 gatcaggcat ctcaaggtcg aggtccgcaa ggtcaactgg ttctcgacct atcacgtcca
     1621 tcatcgggtg acggagcatt ttcgcgaggg ccgcgcattc ctccttggcg atgccgcgca
     1681 catccacagt ccggcgggtg gacagggaat gaacaccggg atcggcgatg ccatcaatct
     1741 ggcctggaag ctggcaagtg tcctcggagg gcgcgcgcct gctgcactac tggacagcta
     1801 tgagcgggag agaatcggct tcgcgcggcg cctggtggcc accaccgatc gattattcag
     1861 cttcgcgacg gccgagggtc gtatcgccga catcatgcga acgcgcgtca caccgttctt
     1921 gtttcccaag ctcgtttcct tcgaccgggt ccgggagttt ctcttccgga ccgtgtcgca
     1981 gatcgccatc cagtatcggg acagcccact cagcgcaggc gttgccggcc aggtgcatgg
     2041 cggggacaga ctgccctggg tccagctcga gggccgggac aacttcgatc cgcttgccgc
     2101 catgacctgg caggtgcatg tgtacggcac gccgcgcgcc gatctcacgc aatcatgcgc
     2161 ggatcggcgg ttacccttgc atgtgtttcc atggagcact gcggcggagc atggcggact
     2221 tgcgcgggat gcgctctatc tgctcaggcc cgatagttac gttgcactgg cggatgcttg
     2281 cgcagatccg ggtgcgatcg agcgttactg cgccgagcgg ggaatccggg tgtagtcctc
     2341 ggacagcgtc tgcgagttac gtcggtctcg aaacacgagc ggcttctcga cggccagacc
     2401 cggcacgctt gcgcgctcga ccatctgtgt gaccaggtgg tggtgcggtg agagatcgca
     2461 gaccgggtcc gtgttggctg catccccggt cagaagataa gcctggcagc ggcagccccc
     2521 gaagtcgcgg atcttctcgg ggcagctgcg gcagggctcc ttcatccagg catcacccct
     2581 gaagtggttg aaggcgggtg actcccgcca tatccattcc acggcggcct cacggacatt
     2641 cgggaacgtg aggcctggca gcatgcgggc cgcatggcag ggcagtgctg tgccatcggg
     2701 tgagaccgtc aggaacaccg aaccgagacc gttcatgcag gccttcggcc ggcgctcgaa
     2761 gtagtcgggg acgacgaaga agatcttcat gcgacgatgg tgctcgcgaa atctctgcgt
     2821 caccgcttcg gcatgctcga gctgcgcccg gctcgggatc aggcgctcgc ggttgtgcca
     2881 ggcccagcca taatactggg tgttggcgag ctccacgtag tcagccttca tctcctgcgc
     2941 catgtccagg atctgttcga tatggtcgat gttcaagcga tgcaacacca cgttgagcac
     3001 catcgggtac tcatagcgct tgatcagcgc tgcgaccttc gatttgagct cgaacgtgcg
     3061 cgtgctgctg aggaaatcgt tcacttcccg agtactgtcc tggaacgaaa gctgaatgtg
     3121 atcgagccct gctttcttca gggcttcgat gcgtgactcg gtgagcccga tgcccgaggt
     3181 gatgagattg atgtagtaac cgagcgtgtg ggcctcggca acgatcgtct cgagatcatc
     3241 acgcgagagc ggctcgcctc cggacaggcc gagctgcacg gagccgagcg cgcgcgcctc
     3301 gtgcagcacc cgaatccact cggccgtgct cagttccagt gcggtccggg agaaatcaat
     3361 ggggttgtag cagaacacgc agtgcagggg gcaccgatac gtgagctcca gaagcagcca
     3421 gagcgggggg cccagggaag cggtcgaggc ttgcgccccc agggggctgg tcggctcgag
     3481 gctcattcgc gcagttccag ccagtgcttc tgcagcgcca ccgctacgaa ggccgtcaca
     3541 tcggcggtga gaccatcagt cgagaaggca gcctccagct ctgcgacgat ctcggccacc
     3601 gtgcgctcgc cattgcagcg gcggaggatc tcgcccgcac tgccgttgag cttcaccatt
     3661 ccttcgggat aaaggaggac gtgagcgttc tgcgccggct cccactgcag ccgcaatcca
     3721 tgccctagag tcggtttcgc ttgcagtccg gcggggctgc tgggctcagc accgccgcca
     3781 gccgcgctca tgagcgaact ccgtagcgct gttgcatggc gtcgttcatc tgccagagca
     3841 cgtccagctt gaactgcagg atctgcaggg cgcgctcctg agcggcgcgg gtgacgaaat
     3901 gatccagcgt gaccgcgagt ccgaattcca cgtcgcggcg cgcaagactg acacggcttt
     3961 ggaagtaatg caggcctgac tggtcgatcc aggggtagtg ctgtggccaa ccggccaagc
     4021 gctgcttgtg aatctctgga gcgaacagct ccgtgagcga ggcgcagacc gcttcctgcc
     4081 aggcagagtg gcgagcgaaa ttcacgtaag catcgaccgc gaagcgcacg ccgggcagca
     4141 cgtcggcgag gctctccacg tgtgtgcgcg acaagccgac tgcttcggcg agccgcagcc
     4201 acgaatcgat tccaccacga tcctctccgt agccatcgtg atcgaggatg cgctgcaccc
     4261 aggagcggcg gacggtgcgg tcagggcagt tggccaggat ggccgcatcc ttgatcggga
     4321 tgctgatctg ataataaaag cggttggcga cccaaccgcg gatctgctcg ggcgaggccg
     4381 cgcccgaatt caacatcaca ttgaatggat gatggatgtg gtaggcgcgc cccttctcgc
     4441 gcagctgctg ctcgaactct gtgcggctcc aggccgtggc cgtcacaggc tcagctccat
     4501 gccatcccag gcgacttcga cgcgacggcg cgccagctcc gccgcctcgc gggagtcttc
     4561 atccaggatg gggttcgtgt tattcacgtg aatcagaatg cgtcgcgtgc ccgagggcag
     4621 cttgtcgagc cattccagca tcccaccggg accgctctgg ggcagatggc cgatctggcg
     4681 cgcacgcttg cgggaggctc cgagccggat catctcgtca tccgtccaga aggtgccgtc
     4741 gagcagcaca caggccgcgg actgcatgtg ctgccagagg cgctcgtcca tgctgcccaa
     4801 acctggcgcg tagacagccg agcctcccgt cagctcatcg gtgatcacga gcgcaaggtt
     4861 gtcccccggt accgggtttt cgcgattggg ggagtagggc gcgggcttgc cctcgatcgc
     4921 cagcgcctgc cactggactc ccgcaacgcc ctcgacggtg aagcgctcgc cgtgcaggtc
     4981 gatcctgcgc cgctccgtac cgcagaaatg accgagcacc ttgaaaatgg gattgccaac
     5041 cgtcaggtcg gtgtaggcgc tgtccgtgca ccagatgcgc cagggccggg aggactcgcg
     5101 gagcatgaag aggcccgtgg tgtgatctac ctgggcgtcc acgagcacga tcccggcaat
     5161 cgcgctatcg cgcagtgtcc ggcccgcctg cagcactgga tttgcctgaa gttgtgcgcg
     5221 gatgtccggc gaagcgttga ccagcgccca tgagctctcg cgggtgccac agaccgcgat
     5281 cgaggattgc gtccgggcgc gcgcccgaat ggtgccggcg cgcagcccgg cgcaattgcg
     5341 acagttgcag ttccactgcg ggaagccacc gccggcggcg gctcccagaa ctctgatctt
     5401 cacgcccggc cagtgaggta gcgcagcccg cttgcgcggg ctggcgcccg gtccatcgac
     5461 tatctgttcg cgatgtacat cgtgatttcc atgccgaacc gcatctctgt ggcttgcggg
     5521 gtttcccatt tcatggctgc tattcctctc gattgatggc ccgcatgatg atcctgcgcc
     5581 gccgctcgcc ccgacagggg acgatcatga aaggcgcctc atgattttca tgagacgaag
     5641 ggagcccccc agcgcgggcc ttcccgtcga ggcgcgcagg accgggaggg cggctggccc
     5701 gctgccgctc gagtggacac gtagcgaggt ttttcctgcc atgaggtgcc gcagggggtg
     5761 ggcggaaggg ctcagacagc cagggtcccg agtcaccccg cggcacgggg ggcggtacac
     5821 tgccccgaat gaaactgcgt acgcgcctca atctcgtcgt caccggcctg accgcgatgt
     5881 tcgttctggt gctgattgcg gcggagatcg aggacacccg ttcttcggtg cgagaggaga
     5941 tcgaagccgc aaaccgcgtc ggctcgcagc tgctcgggag gctcgtcgcg atctattcgc
     6001 gcgaaggcgg accgcaggtg gttctggagt tcctgcagca gctcgggcga gtgagggcga
     6061 acgaagtcag cctctgggcg ccgggcgggc gtgtgctgta tcgatcgccg ccggcaacgt
     6121 acaaggcagg gcgcgaagcg ccgcgctggt tcgagcacct gctggcgccg caagcggctc
     6181 gacatacctt ttatctgccc gatggcgtgc agctcgtggt ggaggccaat ccgtcacggg
     6241 ccattctgga tgcctgggac gacctgacgc gcctgttcga gatcgggacc atcatgctgg
     6301 tgctcgtgag cggcctggct ttctggctgg tcggccgggc gctcgcgccc tttccggtca
     6361 tcgccatggg actggcccgg atcgaggcgg gtgacctcgc ctttcggctg ccaccactgg
     6421 cgggtgccga agcgcatgcc attggcgctg ccttcaatca gatggcccag gcagtgcagg
     6481 acaaggtacg cgttgagcgt caggctcgag aggcggagag ccggctcgag gagcggcgcg
     6541 agctggcgcg cctcgtggag cagcgcatcg aggaggagcg acgtctcatc gcacatgagc
     6601 tccacgatga gttcggacag tcggtcactg cgattcgctc gctcgcaatg gccatcagca
     6661 cacagtcgaa ggatcccacg atgaccgatg ccgcgcggct catctcgcag gaggcgagcc
     6721 gtctttacga tgccatgcat ggcctcattc cgcggctcac accgttgtcc ctcgacacgc
     6781 tcggtctggc cgataccctg gaaaacctgg tccgcgactg gcagcggcgc tacccggatg
     6841 tcgccttgtg tgcccggcat gcactggctg cggagctggg tccgagtgtc acgctgacga
     6901 tctaccgggt ggcacaggag ggcttgatca atgcgctgcg gcatgcgcac gcgacgcggg
     6961 tggagattga tctgcacgcg cgtgaggatc gcatcgtgct cacgattacc gatgatggag
     7021 taggtctgcc ggcggactgg gcccggccgg ggcacttcgg attgcggggg cttgcggacc
     7081 gtgtggagaa cctggggggc gatttcaagg tgggcaactg cgagccgcac ggcgtacggc
     7141 tgactgcaga gattcccctg gtagcgggcg catgatccgc gttctgctgg tggacgatca
     7201 cgccgtcgtg cgcaccggct tccgcctgtt gctgcagtcg cagtccgata tcagcgtcgc
     7261 ggcggaggcg cattcgggtg aggcggcgtg tcagatctat gccgagctga caccggacgt
     7321 ggtcgttctt gatttgggga tgcccggtat gggcgggctc gaggcgctga agcgcatccg
     7381 cgcgcgccac gccgccgcac gggtactcgc gctctcggcc cacgatgacc cgatgcatgc
     7441 ccgccgcgcg ctgcgcgaag gcgctctcgg atttctctcc aaacggagcg cgcccgaggc
     7501 tttgatcgag gcgatcgcct ccgtcgcagc aggccgccgc tatatcgatc cgaccctggc
     7561 tcagaagctc gctctggcgg agatcgaagg tgcggcgcgc tcgcccatcg agcatctttc
     7621 cgagcgcgag ttcgaggtat tcatccggct cgccggcggg gccaccgtgc agaagatcgc
     7681 cgaggatctg aagctctcgg cctcgacgat cggcacgcac ctgtacaaca tcaaacagaa
     7741 gctcggcgtg gtgaaccagg cggagctgac gctgctcgcg atccggcacg gactgatcga
     7801 ggcgtagggc gaaggtcacc cctgcctgcg gttcgcgcca taatggcgct ttcctgtcgg
     7861 ctgctcgagg agccagacat gtccagaggc ttttttgcag cgatcggggc ggcgctcctg
     7921 tggaccggcc cctgcggggc cgagaccgat caggcctccg atgcgctggc gcgcgacata
     7981 ttcaagcagc tcatcgagat cgatacgact gagtcgtccg gcaacgtgac gaaagcttcg
     8041 gaggcgatgg cacaacgcct caacgccgcg ggattcgccg cttctgagat gcagattctc
     8101 ggccccgatg atcgcaagaa gaacctcgtg gtgcgcctca aggggaccgg caagcacaag
     8161 ccgatcctgc tgatcggaca cctggacgtc gtggaggcgc gccgctccga ctggagcaca
     8221 gatccattca agttcgtgga gaagaacggc tacatctacg gccgtggcac gcaggatatg
     8281 aagaacggcg atgcgatcat gctggccacg ctcatccggc tgaagaagga gggcttccgc
     8341 ccgagtcgcg acatcatcct ggcgctcacg gcggatgagg agggcggcag ctccaacgga
     8401 atcgagtggc tgctgcgcca tcaccgggag ctggtcgatg cccagttcgt gttgaaccac
     8461 gattccaact cggtgctctc cgagcacggc aagccggtgt acttcgagat ggacgggacg
     8521 gaaaaggtgt acgccgacta ccagctcact gcggtcaacc ggggcggcca cagctccgtg
     8581 cccataccgg acaatgccat ctacgagctc agcgccgcgc tcatgcggct ctcgaagtac
     8641 gaattcccgt tcgagctcaa cagcgtcact cgcgggtatt atgagcgcat gtcgagcatc
     8701 gagaccggtc agcgcgccgc ggacatgcgc gccatcctga agaatccgcc cgatacggca
     8761 gcgagcgagc gactgtcacg tgatccggtt gatcactcga tgatgcacac gacctgtgtc
     8821 gcgacgcgcc tggagggcgg gcacgccaac aatgccctgc cgcagcgtgc ccaggccacg
     8881 gtgaattgcc ggattttgcc cggacattcg cccgaggagg tgcgcgggat tctcatcaag
     8941 gtcttggctg atccgaagct ccgggtgcag tacatcgatg agtcgggcca ggtgcaggat
     9001 acggcacccg gccgccgcgg ctatgcgcct ccgccactcg atccgcaggt catgcagccg
     9061 ctggacacgg tagtggacca gatgtggccg ggtttgaaag tcgtgccggc catgtccacc
     9121 ggatccagcg atggtctctt cacggcggcg gcgggactgc ccacctacgt aatctccggg
     9181 gtcgcggtgg atcgggacaa cgatcgcgca cacggccagg acgagaatgt cggggtgcag
     9241 tccttctatc acggcaatga attcttctac cggtacatca aggcactcac cgccgactga
     9301 ggccccgtgg ccggcggcgg cggttgcggc gccgggtgca cggccgttgc agctttgccc
     9361 gcgcgagttg ctgctcgggc atctgcaaga tgctctggct gcgggtgacg gccgcgccgg
     9421 tgcgcactgt atccacgagc tctggatgcg gggcgaatgg cctgagcaca tcgagaacgc
     9481 gctgcagcac ctctggcaac acgccgccag atccatccct gagtggctcc cgatgcggta
     9541 cgtgcactgg ttgccaaccg tgtacgaaat agcggctgga tttcacgcgg ctcgcagcgg
     9601 acgcaccaat gtctatctcg ttctcctcga ctacagcgat cacggctcgg agccgcatgg
     9661 catctacgtc ggcatgagtc actatgctcc cgcacagaga ttcgaccagc acaaggccgg
     9721 aatccgggcg gccggttgtg tgttgaagcg tggcctcgag gttctgacgg gaccgacgct
     9781 gcacttgcag ttcatcgccc gcaaggaggc ggcgcgtatc gagtcggagt tggcgatagc
     9841 gctggcagag gctggcctgc gggtggaggg cggccactga ctgtgcgcga tgcgcctgcc
     9901 gcgggcgcat actcgcctca tcgacgatcc ggaatggagc aagaccgtca tgagcggagt
     9961 gccgaacaga gcggatatgg cggagatggc gccggagggt ggtcttgcgg gggacgatcg
    10021 agcccgcctg gtcgcagagc tcggtaatcg ggtcggctcc ctgggcgtcg agctcgcgga
    10081 tgtggcgggc aacctggagg aagtgaccaa ccgcgtcgcg aaccaggcgg agcagttcaa
    10141 ggagctgcag cacgccgccc aggccatggt ggcaggcaat cgagagatcg accgggccgc
    10201 caaggagacc cagctcgccg cctcgactgc cgggaccgag atcgccgagt cccgcacgct
    10261 gatcgggacc gcagcccagc acatcggcga tctcacagcc gccgtaggcc atatcgaaga
    10321 acgcctgggc tccttcagcg ccctgctgag gcagatcgga ggagtggcca cggccatcga
    10381 gcggatcgcc aagcagacgc ggctgctgtc tctcaatgcc ggcatcgagg ccgcccgggc
    10441 aggggaggcg gggcgcggct tcgcagtcgt cgccggtgag gtgaagagcc tggccgagga
    10501 gacccgcacg gctacggacc gaatcgggga gatcgtgcga gcgctgggga gtcagatcga
    10561 agggctcatc agcgaaagcg gcgcggcgac acgccatgcc ggacatgccg gctcgggcac
    10621 ggagaaggtc aagggggtga tcacgcgcgc gcacgaggca ttcaacatcg tcggtcgcga
    10681 gatcgacgcg atcgctcaat cggcagcgga caatctcgaa gcgtgcgata ccacgctcgc
    10741 cgagctcgat gagcttgccg tcggcgtgga cctgtcctcc agcaatctgc gacgggcgga
    10801 tcaacgtatc gagcggctgc tgaccctcag tgagacgctc atcgagttca tcgctcagag
    10861 cggggtggag actgcggaca cgccgatcat ccgcctgggc atacagaccg cagcgcgcat
    10921 cggcgccgag ttcgaggccg ccattgctcg cggggagatc accgcggcgc gcctgttcga
    10981 tgagcgctac cgcgagattc ccggaaccaa tcccaagcag ttcctcaccg actacactgc
    11041 attcaccgat cggctgctgc cgcccatcca ggatccgttg cagagaagcg acccgcgcat
    11101 cgtgttctgc gtggcctggg cgaagggtgg atatctgccc acccacaatc ccgaatacag
    11161 caagccgcag ggtcaggatc ccgcctggaa tgcggcaaat tgccgcaatc gtcggctctt
    11221 cagcgatcgg gccgtgcaga aagtcgccac caacaccaag ccgtttctgt tgcagaccta
    11281 ccggcgcgac atgggtgccg gcaagttcgt gctgatgaag gatctgtccg cgccgatttt
    11341 cgtgcacggt cgccactggg gtgcattccg gatcggcttt cgccagtcct agccgtgtcg
    11401 ccgatgcgcc ggagagctga gtgccggtgg tgccgctcgc gtacaagcac cgaacgcgct
    11461 atttgaggga cagctcgacg cggaccttgt ccttgctgtc gtttttcgag ctgttgccga
    11521 tgatgaagac tcgcgaagga tccacatcgc cgccgctcag caaggtgtcc tgaatggctt
    11581 gcgcccgccg ccggccaagc gcttcgagct ggttatcgga gacagtcatg cgaggcagca
    11641 acgcagactg cagctcgagg ttcgccggtc cgaaatccgg cggcggcgcc ttcttctttt
    11701 cggcggcaac gatggcctga gccgagtccg ggagggccgc atccttgccg agctcctggt
    11761 gatagagcgc cacgagcaga tgaaagcgtg cccccggatc gctcagagcc ggatcggccg
    11821 gcgggctggc attcggatct ggtttgtcat cgccagggct cgcagggctc ttgtgccggc
    11881 cactgcccgt accctgctgc accagggcga gcaggcgccg gtcgagctgc gcgagcgcga
    11941 gtcccggccg gtccagctcc ggtgagaagg tcatcggaac atcgagtccg agttgggttt
    12001 tctctttcag cgccttcgcc agcgtggtca gcttgtcgcg ctcgtgcgga tcgaggctcg
    12061 cgctgcccgg gctgaagtca acgtacttca tctccgctcc gccgcccccg aacagatgcc
    12121 cgagtgcggc gaagggtgcc gtggcgacct tcgtgagcag tcccagcacg gccttccaga
    12181 tcagcggtcc gagactgaat tgggggtcgt ccaggctgcc tgtgacgggc aggttcagat
    12241 cgatcacccc attgcggtcc ttgagaaggg caaccgcgat cttgattgga agcttgacgg
    12301 catcgggtga ttcgacgcgg tctcccagct cgagctggtt gatcacgaag cgatgctggg
    12361 ccgtgagctg gcggttctcg atcttgtagg agatgtcgac ggacagcttg cccttctcga
    12421 tcttgtagcc ggcgaagtgg ccggaatagg gcgtgacggt gctgagctcc atgcctttga
    12481 agctcatgtt gagatcactg taggcagtcg ctgcgagcgg attgacggtg ccccagatgt
    12541 gaaccggcgc gtagcggtcc acctttcctt gcagctcgac tttggcgcgg gcgcgcggat
    12601 ccgacgacag gcctgtgatc gagccctgca gggtctggat gccgacggca aaatgaggct
    12661 gtatccagta atcggagtaa ttggccgatc cgttctcgat acgcacgcta tcgattgcga
    12721 gcgccagttg cgactgtgcc gcggaactcg tggaggctgt ctttggggga tgtcgatgcc
    12781 gccggtcgga tgacgttgct tttggattca ccggcgccgg cgttgcctcg gacgaacttt
    12841 ccgtcgcggc gttcgcagcc gattggctgt tcgacggggg aggctgcggc tccatgacag
    12901 aggcagttgc ctccgcggag attggcgccg cgcctgtgga ctcggctggc ggctcgcccc
    12961 cgtctggttt ggacgccgac ttcagcagca gcaccttcga aacgttcacc gtccgatcgg
    13021 actctatgat cacgcgcgca taaggggccc gagcgaggat cgaatgcact cgcaaactgc
    13081 gcgggctgga cttgaagtcg ataccggaga gctgcagccg ttcgaacttg atgaagtcct
    13141 gcttcaatac gttatcgaca gtgcggacac gggacagctc ggcatccccg gcggcactca
    13201 ggttgccatc cgcgccgcga tcgacgctca gcttcgcaga gagaagaccg ctgcgcagcg
    13261 tcatcgcagt gtgttgcgcg atgtatggct gcagtgccgt caaatcgagc tgtcccacct
    13321 cgccctgcag cttgatggcg gaatcggggc tgagatccgc tttggcatcg agcttgcctt
    13381 catggttgac gtgagtggag agggttacgg tgagcgggct cgtacgcggc aatctgaggc
    13441 cggtcacgtg caggctcagt ccttcgagtt ccagggaagc cgcgggagtg acttgccggt
    13501 cctgtgccga cacatgcagc ccatccaccc gaacgtcggg tactgaaacc tgccacgtgc
    13561 cggaggcgct cccggatgcg gccggcgcgg tgggcgcggt gggcgcggtg ggtgcggtgg
    13621 gtgaggtcgc agctggcgag gattgcgagg gcgccgttgg ggccggcgcg ctgctctgag
    13681 cccccgaggg ccccgatgac aactccagga tgttcaggtt cccgctggaa tcgagccagg
    13741 tgcggatcgt gcccccggcg aggtgcacgt tgccgaccgt gactgaccgg cttgccaggt
    13801 cggcgcgcgt ctccgcgatt tccaccttgc cgagcttcac gtagtcctcc gtgcctccct
    13861 tgggccgtag cgcgagatcc gacacagtca gatcgtgcac attgaccgca agcccgactg
    13921 gagaggtcga tgcggtgaag ttgtagtcac cgtgcaggga gatggcaccc gagggcagct
    13981 cgaaatgcac actgtcccga acgtagttcc agagcgtgtg cgcctggaga ttggccactt
    14041 caaactggcc gtgggagctc aaagggctgg cggccagcga gccggcccac ttgaactgct
    14101 cgccggcttc cgaggtggca ctcagggaat aggagccggc ctcgttgccg accgtgctga
    14161 aatcccgcag ctcgaagttg atcggcttga tttccgcacg gaagacactt ggatgcgccc
    14221 ggtcctcgaa ggcaacgttg ccaccccgca cgctgaagtg gttgatgaac aggcggccag
    14281 gtccggagct cggcttcgag ctgggcttct ggcctgaggc cagatcctgc aggttcagct
    14341 tgccgtcggg cctgatcagc actcgggcgt acggctgctc gagcacgatg gcctcgaagt
    14401 ccggtccgag ccgccagagg cttgcgatgg tggcgtccac catcaggtgc cggaaggcaa
    14461 tcatgggctg cccgtcagag tccggcagcg tgaaatcgct gacatcgagc cgcagggtga
    14521 acggattaaa gcggatgaca ccaagcacaa cgcggcgctg gtagtgctca gagacatagt
    14581 cctgcacgcc ggagcgcagc aggcgcggaa cggccaggaa gcccaggccg gcatacaacc
    14641 ccacgagcag caccacgggc agcaggaccc acaacagcag ccgacgcacg cgcagctcgt
    14701 tcatgatggg cactctatgc cagcccgaga aacagctcca gaaccgcaag tccgcgcgta
    14761 aaggcgcgcc gctgggccgg cgacatgcag gcgagcagct cgcgctcgcg atcgagagcc
    14821 aggggcacga gcgcctggta caagcggcgg cccgacctgc tcaggcgcag ctccatctcg
    14881 cggcgatcgt cggcgctcga cgcgcgctcg atcagacctc gcgccaccag gttggcgatc
    14941 gcgcggctga cccgggtttt gtgcatccgc gtactgctgg cgatgtgctg cgcagtgcag
    15001 ccacgcaccg gaccgacggt agccatgact cgccattcgg ggatctccaa tccgaagcgc
    15061 tcgcggtaga tctccgccag gtgatcactg acggctgctg ccagacggtt gagcctgaag
    15121 ggcacgaagg ctgccaggcg cagggcgggg cgggcgatgg gtgcagcgct caaggtcctc
    15181 tcccttcgga aagttgcgtg cgcaactaat tgtgccatga tatgcgcatg agcgcctcac
    15241 cgaagccgac ggccggcgcg gatcccggcg gcctgcagtt ccagcaggga ttcggcaatc
    15301 acttctgcac ggaggccctg ccgcaggctc tgccgcaggg acgcaactcg ccgcaacgct
    15361 gcccctatgg gctgtacgcg gagcaattct ccgggaccgc ctttaccgca ccgcgacatg
    15421 cgaatcgccg cagctggctg taccgcatcc gccctgctgc gctgcatggg ccgtttcatc
    15481 gcatcgaaga cggcagcctg acgagcgact ttacggaact g
//
LOCUS       DASRHN010000378        17562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_962601, whole genome shotgun sequence.
ACCESSION   DASRHN010000378 DASRHN010000000
VERSION     DASRHN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962601"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1829..1905)
                     /locus_tag="VFB37_15235"
     tRNA            complement(1829..1905)
                     /locus_tag="VFB37_15235"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1869..1871),aa:Asp,seq:gtc)
     gene            complement(2213..2689)
                     /locus_tag="VFB37_15240"
     CDS             complement(2213..2689)
                     /locus_tag="VFB37_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO23855.1"
                     /translation="MSNRQPLQIPQSTIRLRLVMLALTATASIAVGSDVQAVDAPRPA
                     VVSTLMTHELSGIAGKEAVVLTVEYPPGGSSPPHRHDANVFVYVLEGAINMQIEGQKQ
                     VTLTAGQTFYEGPWDVHVVSANASTTQPAKFLVVMVKDKNAAVGRPVGSGPSTPPH"
     gene            complement(2709..3008)
                     /locus_tag="VFB37_15245"
     CDS             complement(2709..3008)
                     /locus_tag="VFB37_15245"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZO23856.1"
                     /translation="MFWEIAQIDIKPGEEGAFESALAQAVPLFRDARGCRAMQIQRSV
                     EHPSRYRLVVDWETVEDHTVHFRGSEAFTRWRQLVGPHFASPPVVEHTRRLPIGF"
     gene            complement(3394..3696)
                     /locus_tag="VFB37_15250"
     CDS             complement(3394..3696)
                     /locus_tag="VFB37_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZO23857.1"
                     /translation="MSPRSHRSGPKPPSLVVNLVVLAALLGFLALTVGTAYINMGPAN
                     VVANVGIAVAKAILVLTFYMRLKSDSPLFRIVAVSGFAWLAVLIALSLADLLTRPA"
     gene            complement(3693..4334)
                     /locus_tag="VFB37_15255"
     CDS             complement(3693..4334)
                     /locus_tag="VFB37_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HZO23858.1"
                     /translation="MSTTPTAVQFDDAVQQREAATLGMWTFISTEILFFGVLFAAYTL
                     CRTRFPEAFAEGSRHTDVLLGTAETAVLLISSCLVAVAVREVQLGGQRIATWLIAATA
                     ALGVCFLTMHGFEYVSEYHEGLVPAIRYTQSGPSAGQMEIFFCLYYFITGFHGLHVTI
                     GVIVLLVMARRTHRGDFDQDYHTPLEMSALYWHLVDIVWIFVFPLIYLIGRSG"
     gene            complement(4331..5971)
                     /gene="ctaD"
                     /locus_tag="VFB37_15260"
     CDS             complement(4331..5971)
                     /gene="ctaD"
                     /locus_tag="VFB37_15260"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZO23859.1"
                     /translation="MSVNVADSPFPTEQGTDEPNPESYLDAGYGLRSWLTTHDHKRIA
                     ILYALAITFFFFAGGLAAALMRLELISPNGSLMSAEAYNKTFTFHGVVMVWFFLIPSI
                     PATLGNFLLPLMIGARDVAFPRLNLFSWYLYITGGLFTIACLLFGGVDTGWTFYTPFS
                     TMFSNSNVMLTVVGVFIVGFSSILTGLNFIVTIHAMRAHGMTWFRLPLFVWSMYGVSL
                     VMVLATPVLAMTLALIGLERLLGVGIFDPALGGDPLLFQHFFWFYSHPAVYIMILPAM
                     GVASELITAAVRRRIFGYSFMVYSILGIAVIGFLVWGHHMFVSGQSMYASIVFSILSF
                     IVAVPSAIKVFNWTATLYKGDITFGAPMLYALGFVGLFMLGGLTGLFLAATALDVNLT
                     DTYFVVAHFHYIMVGGSVMAYLGGIHFWWPKITGRLYSEAWARFAAIVMFFGFNLTFF
                     PQYLLGYEGMPRRYHVYPPQFQVLNVLSSAGATILAVSYLLPLAYLLWSLRYGPRAGA
                     NPWRAKGLEWQTSSPPPVHNFDQTPTVTEGPYLYDESP"
     gene            complement(5968..6891)
                     /gene="coxB"
                     /locus_tag="VFB37_15265"
     CDS             complement(5968..6891)
                     /gene="coxB"
                     /locus_tag="VFB37_15265"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZO23860.1"
                     /translation="MSSLPQASSFAGQVDYLFDAMLALSAVVVIGVFAAILWFAVKYR
                     HGSRADRSRSSRRTLGIELAWTLIPLFLFVGIFAWSISLFAYMSRPPIETVPVYVVAK
                     QWMWKVQHPGGQREINTLHLALGQPTRLVMTSQDVIHSFYVPAFRVKQDVLPGRYTQL
                     WFNATRAGTYNLFCAEFCGTDHARMGGLVIVMQPKDYANWLEAHAGTDLAAQGAALFR
                     RLGCSGCHDPQSGVHAPDLYDLYGSTVPLSDGTQVRADERYLHDSIMLPNKEIAAGYA
                     PVMPSYQGRIGEDDVLALIAYLKSRHAAGSP"
     gene            complement(6888..8063)
                     /locus_tag="VFB37_15270"
     CDS             complement(6888..8063)
                     /locus_tag="VFB37_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZO23861.1"
                     /translation="MSQRDNHESYEVFAGRLHWLAALLVGSLVGIVWLMYVLSKTWLG
                     PPPEAPRGAAIPTPRLQPNPHIDLAHERSREQALLERYQWVDRQAGIARIPIERAMQL
                     LAGSAHGPATPSGPPASAAPLAPPAPAAQSQPSASSIPVKPTSQGSQVALPPPKDLYR
                     RAGVDQHLGVQVPLRLLFRDSDGRAITLQALTHGKPTLLALGYYRCPNLCDMTLHGMG
                     RAAAAMPLRVGRDYQVAFVSIDPGETPRDAHATAAMLEHDVPAGQPDRWHLLTGTQSA
                     IRGLSEATGFRSFRDDRIGQYAHAAGVVVLTGQGLIAQYFFGVSYSPRALRLALVNAS
                     GGHLGSILDQLVLLCCAYDPSTGRYSLLIGKIMQALGIVFVLGAGALWWTLHRGRRA"
     gene            complement(8060..9148)
                     /locus_tag="VFB37_15275"
     CDS             complement(8060..9148)
                     /locus_tag="VFB37_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23862.1"
                     /translation="MSGGTSRLRVSILVLGAACLLGCALIAIHAPEQAARSYLAAYFF
                     FLAPALGGVALLMIHALTGGEWGMDLRPALLAASGVLPVLAVLFLPVLLSAHGLYPWV
                     APSAQSPDALGPQFARQQWYLNLPFFVARAVGCFVVWMWVARGLRRRLSMPGPGVGLT
                     RFAALGLIVYLLSVTIAAVDWVMSLTPAWHSSTFGLLIGTGQLLAAAALAIGTATRLH
                     PVARPRDLGSLLLALVLAWAYLAFMDYLTAWSADLPGETVWYLPRVRTTWQWLAVMLI
                     VCQILVPFALLLSPQFRQSRPGLRAVAGLLLFSQAIYALWIVLPGFRPHGVVLHWTDA
                     LAWLGIGGVCWAVFDLRLGATRRRELSA"
     gene            complement(9145..9603)
                     /locus_tag="VFB37_15280"
     CDS             complement(9145..9603)
                     /locus_tag="VFB37_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZO23863.1"
                     /translation="MYEQPKYKPGSASSLFADGSSSRAPPAGSVPIHSEGEPQQPPVS
                     LSLLQRGRERFDIYCAPCHGESGHGDGMVVERGFPAPPSFHTDVLRKAPDSHFYDVIS
                     DGYGVMYPYSDRIAPADRWAIVAYIRALQLSQNAPRNRLDARDLEELGGR"
     gene            complement(9672..10208)
                     /locus_tag="VFB37_15285"
     CDS             complement(9672..10208)
                     /locus_tag="VFB37_15285"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HZO23864.1"
                     /translation="MSGAAEYGSLAEFASVEALRAAVGGLRKRGYTRLEAYTPFPVEG
                     LAQAIATSRDWTAVAMLIGGLVGGVGTLALQYYSAAINYPIDVGGRPDASWPAFIPAA
                     AEMTILFAALAGVVSMLIGNGLPRLHHPLFELDRFSKASRDGLLLVVRADDPHYDGEA
                     VRRDLSELDATHIETVPS"
     gene            complement(10205..11545)
                     /gene="nrfD"
                     /locus_tag="VFB37_15290"
     CDS             complement(10205..11545)
                     /gene="nrfD"
                     /locus_tag="VFB37_15290"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZO23865.1"
                     /translation="MNASAQPLGLDLQAVTDPVSNIVLARAPGRGWLLAFVASIVLTA
                     LLGSGIWVLLVRGVGIWGIDIPVAWGFAIADYVWWIGIGMAGTFISAALYLTRQSWRS
                     SLNRYAEAMTVFAVSVSGLFPILHLGRPWFFYWLAPYPNRMALWPQWRSSLLWDFFAI
                     TAYLSVSILYFYVGLLPDLATLRDRARTRGSQVFYGLLALGWRGEARHWRRFETLSLI
                     LAGLAVPLVFSVHSMVALDFSEGLLPGWHTTLFPPFFVAGALFSGFAMALVLGIPLRR
                     YYGLTDFITEAHLDKLARMLLAVGLVVDYSYVTEVFFAFYGGDRYEIALTLNRLGGAY
                     SWIFWTTILCNVIAIQLLWLRRVRANHIALFSIAIVVVIGMWFERFMLIVTSLYRSFL
                     PSSFGMFYPTLWDLGFLVGSIGLFLLLFLLFARLLPVVSMFELRKAVGSRSRSS"
     gene            complement(11542..14508)
                     /locus_tag="VFB37_15295"
     CDS             complement(11542..14508)
                     /locus_tag="VFB37_15295"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZO23866.1"
                     /translation="MPTPGSAALEAIRRELAGKPGGFWRSLEELLEQPQMLQRFKEAV
                     PQAAMLDPVLDRRGFLGLLGASLALGGLTACSGPPAEQIVPWIRTPEGMLPSQPRFYA
                     TTLQVGADVIGVLVETHEGRPTKLEGNPEHPASLGSTDPMTQAAILELWDPDRSQAPL
                     RHGVLTGWDDFQAEAIAAVRQYVRDGSGLHVLSGRIESPTLAAQREELLHRYPGARWH
                     QYEAVDEDSALEGALAVSGRPLRARHRFDRAEVIVSLEADFLASMPGHVRAAREFAAR
                     RRPVGSPAKMNRLYVLEATPSLTGSNADHRWPLPTGDISALAAALAVRLGVSHPTLTG
                     ESAPGSPGAAARRLGALAQDLLSNRGACLVIAGASQPPLVHALAILMNRALGNIGQTV
                     EYFEAPEPRESSGEALRSLAESMGAGEVDTLLMLDVNPVYAAPADVRFAERLARVRRS
                     IHVGLYRDETAARCTWHLPRTHALESWSDLRAFDGTASIVQPVIDPLYDVRSVHEILA
                     FMLESTRTTAREIVRNTWSHQLGLNDEARWQSALRQGVIENSAPPAVEPRGNTSSLQR
                     LSRPRPQAQIDLLFRPDPTIWDGRYANNAWLQELPKPTNQLTWSNAALIGPAFATRHG
                     LRNGEVVSLRTEGREVQAPVWIMPGQADNTVVAPLGYGRTIAGHVGERLGFDAYRVRG
                     LSTTWRAECTIASTAKHIELVTTQRHHQMEGEPPVRTGTLAQFRDNPAFLQGASKTPP
                     SLYPPRPRVEYRWGMSIDLNACIGCGACTLACQAENNIPPVGAQEVARGRELHWIRVD
                     RYFEGTPAAPRVHHQPVPCMHCGHAPCELVCPVGATVHDSEGLNVQVYNRCVGTRFCS
                     NNCPYKVRRFNFLQYADVSSPTLKASRNPEVSVRMRGVMEKCTYCIQRIERAHIEADK
                     QGRRIADGEVVTACQAVCPAKAITFGDTADPATAVSREKASQRNYALLEELNTRPQTT
                     YLGSLRNPNPAFEDDT"
     gene            complement(14508..15155)
                     /locus_tag="VFB37_15300"
     CDS             complement(14508..15155)
                     /locus_tag="VFB37_15300"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZO23867.1"
                     /translation="MAQIFSRRFGTLLKLTLVVGAVLFTGSVLSWRALTADPHAMGEP
                     VEQPVPFSHKHHVGDVGLDCRYCHASVETDAVAGIPPISTCMTCHSELFTDQAILAPL
                     VRSWQTRKPLLWQRIYDLPDFVYFDHSIHIAKGIGCVSCHGRVDRMPLMARQASLSMH
                     WCLDCHRNPEKFLRPRSQVFSMDWSPQDQSTLGESLLARYHIDKRRLTDCSVCHR"
     gene            complement(15222..15875)
                     /gene="queE"
                     /locus_tag="VFB37_15305"
     CDS             complement(15222..15875)
                     /gene="queE"
                     /locus_tag="VFB37_15305"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HZO23868.1"
                     /translation="MAYAVKEIYYTLQGEGAHSGRPAVFLRFAGCNLWTGLERDRAKG
                     PGGCSRWCDTDFVGTDGPGGGKFASAGQLVSAVTRHWPAGGREGSALPFVVCTGGEPL
                     LQLDAPLLAQFNSSGWQVAVETNGTVLPPAGELWITVSPKAGATLRVLQGDELKLVYP
                     QVGAEPERFAALHFEHFFLQPLDGPERARHTELAVQYCLGHPRWRLSLQTHKILSIR"
     gene            complement(15964..16671)
                     /gene="queC"
                     /locus_tag="VFB37_15310"
     CDS             complement(15964..16671)
                     /gene="queC"
                     /locus_tag="VFB37_15310"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005399706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZO23869.1"
                     /translation="MAIAERKAVVLLSGGLDSSTVLAIAQSQGFSVCAMSFRYGQRHI
                     VELGFAQRVARRAGVLRHVVVDIDLRQFGGSALTDDLEVPKDRPEQDMAAGIPITYVP
                     ARNTVFLSFALAWAETLQAQDIFIGVNALDYSGYPDCRPEYIAAFERLANLATRAGVQ
                     SGESFRLHAPLIELTKAQIIQRGLALGVDYGLTSSCYDPGADGTPCGACDSCVLRAKG
                     FREAGVLDPLLSRTEPR"
     gene            complement(16690..>17562)
                     /locus_tag="VFB37_15315"
     CDS             complement(16690..>17562)
                     /locus_tag="VFB37_15315"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HZO23870.1"
                     /translation="RILGCAAFACGATPAYSWGDEGHEIIGLIAERYLDPAVRTRVNS
                     ILAADHSALTRDDRIDQEATWADKYRDSDRGTTKMRYEATRQWHFVDLELDGPDLKAA
                     CFGEPPLPSGTAASRGPAADCVVDKIDEFVAELKSPATSAPERLMALQFVLHFVGDVH
                     QPLHASDDHDQGGNLKIVMAPGLAPNSLHHDWDTAFVARLGENEAAIAQRLIAGITAA
                     QRTKWRAGSAADWARESFAVARDHAYGLLPPASSANLYNVSSAYVTDATTVTAEQLSK
                     AGVRLAYLLNQSLR"
BASE COUNT         3491 a         5634 c         5627 g         2810 t
ORIGIN      
        1 ggcaaccgtc ttgccgtaat agactccgac atgccggagc tcctccggcg gcgtggcctg
       61 tggaaatcga cggatccgct gcccactccc actgtgatgc ctaagggctg cgcaaagctc
      121 gtgctgcgca aaggcgagga gtggtgcgag tgaggccgga tggggccggc cacgccggag
      181 tggggttggc ttcggccgtc atcggggtca tcctcatcaa tccttcccta taagttgaac
      241 gaccaagacg cgccaggccc tctgtcccct actcgggctt gcgggcgtcc gcgatgcgca
      301 gcacgtgcga ggcgagcatg accatttact gcgcttcgct tggattgacc tgggtcccga
      361 ttttgggtca attcagagct gggctaaagt cacggactcc tccccgctcg atacaggatc
      421 gccgcatggg ccgcatctcg atctcaggag gcgtcaggcc gaaggggctg ggccgcattc
      481 aatttgattt tgtgatggac gggattcgac tacgaccgtc gctttcatgt gcaccgactc
      541 ccggcaatct ccagaaagcc cgcaagctac ttgcacgggt caaggcacag atcgacgccg
      601 ggacgttttg ctttgtcgac accttcccaa actttcgagc gctgaagaag ctgccgcctg
      661 gtatcactgc tcggagctgc ggtgaggtat tcgatgactt cctgaatcat gaagcggcac
      721 gcgtcgcgcg gggcgacctg gctccgatca cactccgctc tcatcgacag atactcaacc
      781 acgcgtggcg tccgcagtta ggcgccctgc ccttcctcgg cgtcccgcac tccaggctaa
      841 tcaagattgc ggattcgcat cagtggaaca agaagaccta taacaacgcg gtcagtgcat
      901 tgcgtcgtgc attcgcgttt ggatatctcg attacccagg ccatcacaat cccgccgatg
      961 acctgcgtgg ggcacgcatg ggcaaaaagg accgacctcg aatcgacccc ttcagcatcc
     1021 aggatgcgga ggtgttgatc aaggctctgc acctcgattg gggtcatgct cacggcaatt
     1081 atgatgagtt tcggttcttt accggcttac gtccttcgga ggaaatcgcg ctcgtcgtca
     1141 ccgactacga tcgcgcacag ggggtcctga gtgtgaccaa agcgcgcgtc gatggcattg
     1201 ataaagactg taccaagacg ggcgatgacc gccgaatcaa gctgtgtcca cgggcgatcg
     1261 cgatcttgga gcggcatctg aagcttcgag accggctcgt ggccgaaggg cagattcaac
     1321 acccgtattt attcttcacc gacacgggcg caccgattcc gaatgtgcga tatccctacc
     1381 agcgttggga gagaaccctc aagcgcctcc gcgtccgata tcgtcacccc tacgtggccc
     1441 ggcacacctc ggtgagttgg aacctcatgg caggccgcaa tccgctcctt atcgccaagg
     1501 agcatgggca tcgcatcctc accatgctca cggtctattc agcgtggacc gaaggggccg
     1561 ccgaggcaga tgtcacggcg ctgcgagaag cgatgggcta cgccgtgcct gaagggattc
     1621 gtcacccggc ggtcacgcag cctgctccag tgcctgcact gaccgtagag atccccagct
     1681 ctcgaacccc agagattccc gggattggtg ccacagggaa actaaaacgg actcgagccg
     1741 cgacagagat cgatgatcca tttggcactg aattcggcac tccggagatg gcggagttgc
     1801 tcaagtgcct gatttgctgc aagaagtttg gcggagcgga cgggactcga acccgcgacc
     1861 cccggcgtga caggccggta ttctaaccag ctgaactacc gctccgactg tgggcaagtg
     1921 gctgcggacg gctggggaag ccgccagggg gcgaatccta atgactgccc ctccgggcat
     1981 caaggtattt cgaggttcgg cgctgtcagc gtcccaccgc ccggaggtgc cagggcgtat
     2041 gcgcagggca tggctgcact cgcctaaccc ggttacggat cggtgctcgt cgggctgggc
     2101 ccaggcgggc aagcctggcc actcgcaccg ccccataccg gtgagcgcag ctcgcgaggg
     2161 gccagcgcct tcgcagcaag ctccggggct cagagctcac gctcacgctg actcagtgcg
     2221 gtggcgtgct cgggcctgag ccgacgggac ggcccaccgc ggcgttcttg tccttcacca
     2281 tgacgaccag gaatttcgcc ggctgcgtgg tgctggcgtt ggctgacacg acatgcacgt
     2341 cccaggggcc ctcgtagaaa gtctggcccg cagtgagcgt gacctgcttc tgcccctcga
     2401 tctgcatgtt gatcgcccct tccaggacgt acacgaacac gttggcatcg tgacgatgcg
     2461 gtggcgagct gccacccggc ggatactcca cggtcagcac aacggcttcc ttgcccgcga
     2521 tgccgctgag ctcgtgagtc atgagggtcg agactacagc cggtctcggc gcatcaaccg
     2581 cctggacgtc actgccaacg gcgatgcttg cagtcgcggt cagggccagc atgacgagtc
     2641 tgagccggat tgtgctttgt ggaatctgaa ggggttgacg gtttgacata gaagctctgc
     2701 tggtgaattc aaaaaccgat cggcaatcgc cgcgtgtgct cgaccacggg cggacttgca
     2761 aagtgaggtc cgacgagctg gcgccaccgc gtgaaggcct cggagccgcg gaagtgcacg
     2821 gtgtgatcct ccactgtctc ccagtcgacc accaggcggt agcgcgaagg atgctcgacc
     2881 gagcgctgga tctgcatggc gcggcaaccc cgcgcgtccc tgaagagggg gacggcttga
     2941 gccagcgcgg attcgaacgc gccctcctcc cccggcttga tatcgatctg cgcaatctcc
     3001 cagaacatgc ggccgctcca ctacccgttg ctgtccaggg ccgattctag cgcctctgcg
     3061 cactcccagt tgcgcctcgc tcggatcagc caaagctgac atcggtaagc gcgcgctgcg
     3121 gaagtgaacg ccgctacacg gccgatagga gtccgcgccc gcggaccagg ggcagctggc
     3181 agcctttccg cagaccaacc agctcctgat cagcgacacc tacgccaata tcaagaagct
     3241 cgaggctctg gtccatgcaa tcgacgccga ggcggccaac cgacacgaga agtgctcgcc
     3301 agcgaaagcg ggcagcgacc aggaaggcat gtagggtggt tgcactgtcg cggcggcggt
     3361 accaaggggt tgcgcccggc gcgtgccatc ggctcacgcc ggccgagtca gcaggtccgc
     3421 aagcgacaac gcaatcagca ccgccagcca tgcgaacccg ctgaccgcga cgatgcggaa
     3481 gagtggactg tcactcttca ggcgcatgta gaaggtgagc accaggatcg ccttggcgac
     3541 cgcgataccg acattggcga cgacatttgc cggacccatg ttgatgtaag ccgtcccgac
     3601 ggtcagcgct agaaagccca gcaaggctgc caggaccacc aggttcacga ccagggatgg
     3661 cggcttgggg ccgcttcggt ggctacgtgg gctcatccgg agcgtccaat cagatagatc
     3721 agtggaaaga cgaagatcca gacgatatcg accaggtgcc agtagagcgc gctcatttcc
     3781 agcggagtgt gataatcctg atcgaaatcg ccgcggtgcg tgcgcctcgc catgaccagc
     3841 agcacgatga cgccgatcgt cacgtgcagg ccatggaacc cggtgatgaa gtagtacaga
     3901 cagaagaaga tctccatctg ccctgcagag ggtccgctct gcgtataacg gatcgccggg
     3961 accagaccct cgtggtactc gctcacgtac tcgaatccgt gcatcgtcag aaaacagacg
     4021 ccgagagccg ccgtcgcggc gatcagccag gttgcaatgc gctgaccgcc gagctggacc
     4081 tcgcgcaccg ccacggcgac gaggcagctg ctgatcagca gcacggcggt ctcggccgtt
     4141 ccgagaagca cgtctgtatg acggctgccc tcggcaaagg cttccggaaa gcgcgtccga
     4201 cacagcgtat acgctgcgaa gaggactccg aaaaacagga tttccgtgga gatgaaggtc
     4261 cacatgccga gcgttgcagc ctcgcgctgt tggactgcgt catcgaactg cactgcagtg
     4321 ggggtcgtgc tcatggcgac tcgtcgtaca gatacggacc ctcggtcacc gtaggggtct
     4381 gatcgaagtt atgcaccggc ggcggtgagc tcgtctgcca ttcgagaccc ttggcccgcc
     4441 atggattcgc gcccgcgcgc gggccatagc gcagggacca gagcagatac gcgagcggca
     4501 gcaggtacga gacggcgagg atggtggcgc ccgcggatga gagcacgttg agcacctgga
     4561 attgtggcgg gtagacgtga taacgccgcg gcataccctc gtagcccagc agatattgcg
     4621 ggaagaatgt cagattgaag ccgaagaaca tgacgatcgc ggcaaatcgc gcccacgctt
     4681 ccgaatagag gcggccggtg atcttgggcc accagaaatg aatcccgccc aggtaggcca
     4741 tcaccgagcc tcccaccata atgtagtgga agtgggcgac gacgaagtag gtgtcggtga
     4801 ggttcacatc gagagcggtc gcggcgagga acaggcccgt caggccgccg agcatgaaca
     4861 ggcccacgaa gcccagcgca tacagcatgg gcgctccgaa ggtgatatcg cccttgtaga
     4921 gcgtggcggt ccagttgaag accttgatcg ccgagggcac ggcgacgatg aagctcaata
     4981 tcgagaacac gatgctcgcg tacatcgact gcccggagac gaacatgtga tggccccaga
     5041 ccaggaaccc gatcacggcg atgccgagga tcgaatacac catgaagctg tagccgaaga
     5101 tgcgtcgccg cacggcggcc gtgatcagct cactcgcaac ccccatcgcc ggcaggatca
     5161 tgatgtagac cgccgggtgc gagtagaacc agaagaagtg ctgaaacaag agcggatcgc
     5221 cgccgagcgc cggatcgaag atcccgaccc ccagcaatcg ctcgagcccg atcagggcaa
     5281 gcgtcatggc gagtaccggc gtcgccagca ccatcacgag cgacacgccg tacatggacc
     5341 agacgaacag cggcagccgg aaccacgtca taccgtgggc acgcatcgca tggatcgtca
     5401 cgatgaaatt gagtcccgtg aggatcgagg agaacccgac gatgaagacg ccgaccaccg
     5461 tgagcatgac gttcgagttc gagaacatcg tcgagaaagg cgtatagaac gtccagccgg
     5521 tatccacgcc gccaaacaga agacaggcaa tggtgaacag ccccccggtt atgtacaggt
     5581 accagctgaa gagattcagc cgcgggaagg cgacgtcgcg tgcccctatc atgagcggca
     5641 gcagaaagtt acccagcgtc gccggaatcg agggaatcag gaaaaaccac accatcacca
     5701 cgccatggaa cgtgaaggtc ttgttgtagg cttccgcact catcaggcta ccgttgggcg
     5761 agatgagctc gagcctcatc agtgcagcgg ccaggccccc ggcgaagaag aagaaggtga
     5821 tggcgagcgc atacaggatc gcgatgcgct tgtgatcgtg cgtggtgagc caggagcgca
     5881 agccatagcc agcatcgagg taactttccg ggttgggctc atcggtgcct tgctccgtcg
     5941 gaaacgggct gtccgcgaca ttgacgctca tggcgacccc gcagcgtgac gtgacttgag
     6001 ataggcgatg agcgcgagca catcgtcctc gccgatacgg ccctgatagc tcggcatcac
     6061 aggagcgtag ccggccgcga tctccttgtt gggcaacatg atcgagtcgt gcaggtagcg
     6121 ctcatcggcc cgcacctggg ttccatccga cagcggaacc gtgctgccgt aaaggtcgta
     6181 gagatccggc gcgtgcaccc ccgactgagg atcgtggcaa ccactgcaac cgagccgccg
     6241 gaacaaggcc gcgccttgag cggccaggtc tgttcccgcg tgcgcttcca gccagttcgc
     6301 atagtccttg ggctgcatca cgatcacgag gccacccatc cgggcgtgat cggtaccgca
     6361 gaactccgcg cagaacagat tgtaggtccc ggcgcgggtc gcgttgaacc agagctgcgt
     6421 atagcgcccg ggcagcacgt cctgcttcac acgaaacgca ggcacataga aactgtgtat
     6481 gacatcctga gaggtcatca cgagacgtgt cggctgaccc agtgccaggt gcagcgtgtt
     6541 gatttcacgc tgcccaccgg gatgttgcac cttccacatc cactgcttcg ctacgacata
     6601 gaccggcact gtctcgatcg ggggccggct catgtaggca aagagcgaga tgctccaggc
     6661 gaagatcccc acgaaaagaa acagggggat gagggtccag gcgagctcga ttcccagcgt
     6721 gcgccgcgac gagcgactgc ggtctgctcg actgccgtga cgatacttca ctgcaaacca
     6781 caggatggcc gcgaagacac cgatcacgac aacggcgctc agagcgagca tggcatcgaa
     6841 caggtaatcg acctgtccgg cgaagctgga tgcttgcggc agactgctca agctcgcctc
     6901 ccgcggtgca gcgtccacca gagggccccg gctcccagca cgaacacgat gccgagggcc
     6961 tgcatgatct tcccgatcag caggctgtaa cgcccggtgc tcggatcgta cgcgcagcac
     7021 aacagcacca gctgatccag gatgcttccg aggtggccgc cggaggcgtt gaccagcgcg
     7081 aggcgcaaag cacgtggaga gtacgagacg ccaaagaagt actgggcgat caggccctgc
     7141 cccgtcaaca ccacgactcc ggccgcgtgc gcgtactgtc cgatgcgatc atcacgaaag
     7201 gacctgaacc cggtcgcctc cgagaggccg cggatcgcgc tctgggtgcc ggtgagcaga
     7261 tgccatcggt ccggctgtcc ggccggaacg tcgtgctcga gcattgcagc ggtggcgtgc
     7321 gcatcgcgtg gcgtttcgcc gggatcgatg ctgacgaacg ccacttggta gtcgcgtcct
     7381 acgcgcagtg gcattgcggc cgcagccctt cccatgccgt gcaaggtcat gtcgcacagg
     7441 ttgggacagc gatagtagcc gagcgcgagg agggtcggct tgccatgcgt cagcgcctga
     7501 agcgtgatgg ctcggccgtc ggaatcccgg aacaacagcc gcagaggtac ctgcacgcca
     7561 agatgttgat cgacgcctgc acggcgatag agatccttcg ggggtgggag cgcgacctgg
     7621 ctaccctgag aggttggctt gaccggaatt gaactcgccg agggctgcga ctgcgcagcc
     7681 ggcgcgggtg gagcgagtgg cgcagcggag gcgggtggcc cagagggcgt ggccggcccg
     7741 tgagccgatc ccgccaggag ttgcatcgcc ctctcgatcg gaatccgcgc aatgccggcc
     7801 tgccgatcta cccactgata ccgctcgagc aacgcctgct cgcgcgagcg ctcgtgggcc
     7861 aggtcgatat gaggatttgg ctgcagtctc ggagtgggaa tcgcggcgcc tcggggggcc
     7921 tcgggtggcg gccccagcca ggtcttcgac agcacgtaca tcagccacac gatccccacc
     7981 agcgagccga ccaggagcgc ggcgagccag tggagccgtc cggcgaacac ctcataagac
     8041 tcatgattgt cgcgctggct catgcggaga gctcccggcg gcgggtcgcc cccaggcgca
     8101 gatcgaacac ggcccagcac acccccccga tgcccagcca cgcaagcgca tccgtccagt
     8161 gcaggaccac accatgaggg cgaaagcccg gtagcacgat ccagagtgcg tagatcgcct
     8221 gggagaacag cagcagaccc gcgaccgccc tgagcccggg gcgcgactgt ctgaactgcg
     8281 gcgagagaag cagcgcaaaa ggcacgagga tctgacagac gatgagcatt accgccagcc
     8341 actgccaggt ggtgcgaacc cgcggcagat accagaccgt ctcccccgga agatcggcgc
     8401 tccaggcggt cagatagtcc atgaacgcca ggtaggccca ggcgagcacg agggccagca
     8461 acagactgcc tagatcgcgc ggtcgggcca ccgggtggag acgtgtcgcg gtgccgatcg
     8521 cgagcgctgc agcggcgagc agctgtccgg tgccgatcag cagtccgaag gtcgaggaat
     8581 gccacgcagg ggtcagcgac atgacccagt cgaccgctgc gatggtgaca ctcaggagat
     8641 agacgatgag gccgagtgca gcaaagcgcg tcagcccgac gcccggaccc ggcatggaga
     8701 gccgacggcg caaaccgcga gccacccaca tccacacgac aaaacaaccg accgcgcggg
     8761 ccacgaagaa cggcagattc agataccact gctggcgcgc gaactgcggg cccaacgcgt
     8821 ccggggactg ggcgctcgga gcgacccacg gatacagacc gtgagcgctc agcagcaccg
     8881 gcaggaacag cactgccaga acaggcagca cgccggaggc cgccagcaat gccggccgca
     8941 gatccatgcc ccactcacca ccggtgagtg catgaatcat gaggagcgcg acgcccccga
     9001 gcgccggcgc aagaaagaag aaataagcag ccagatacga ccgggccgcc tgctcgggtg
     9061 catggatggc aatgagcgcg cagcccagca aacatgcagc ccccaggacc agaatgctca
     9121 cacgcaagcg tgaggtcccc ccgctcatcg cccgcccagc tcctcaagat cccgggcatc
     9181 gagccggttc cgcggcgcgt tctggctgag ctgcagagcg cgaatgtacg ccacgatcgc
     9241 ccatcgatcc gccggagcga tgcgatccga atagggatac atcactccgt atccatcact
     9301 gatcacatcg tagaagtggc tgtccggcgc tttgcggagc acatcggtgt gaaagctcgg
     9361 aggcgcagga aagcctcgct ccacgaccat gccgtcgccg tgtccgcttt ctccatggca
     9421 gggtgcacag tagatgtcga accgctcacg gccgcgctgc aggagactca aggagaccgg
     9481 gggctgctgt ggctcgccct cgctgtgaat cggtacgctt cctgctgggg gagctcgcga
     9541 gctgctgccg tctgcaaaca gcgagctcgc cgacccgggt ttgtactttg gctgctcgta
     9601 catgttatgc atagcgcgct cacaccccga gaggccgagc gcacagccga agaggagcgc
     9661 tgccgcgcgc ctcatgaggg caccgtttcg atgtgagtcg catccagctc gctcaggtcg
     9721 cgtcgcactg cctcgccgtc gtagtgcgga tcgtccgcgc ggacgacgag cagcagcccg
     9781 tcgcggctcg ccttgctgaa tcggtccagc tcgaacagcg ggtgatggag tctcggcagc
     9841 ccattgccga tgagcatgct caccacgccc gccagcgcag caaacaggat cgtcatctcg
     9901 gcagccgcgg gaatgaaggc gggccagctc gcatcgggcc ggccgccgac atcgatggga
     9961 tagttgattg cggcggagta gtactgcaac gcaagcgtgc ccacgccacc gacgaggcca
    10021 ccgatgagca tcgcaaccgc cgtccagtcg cgtgaagtgg cgatcgcctg agcgagccct
    10081 tccaccggaa agggcgtata ggcttcgagt cgcgtgtagc ctcgcttgcg caggccgccc
    10141 accgcagcgc gcagcgcctc gaccgaggcg aactccgcga gggagccgta ttccgcggca
    10201 ccgctcatga gcttcggctc cggctgccca cggccttgcg caactcgaac atcgacacca
    10261 cgggcagcag ccgcgcgaag agcaggaaca gcagcaggaa cagcccgatc gagcccacga
    10321 gaaagcccag atcccatagc gtgggataga acatgccgaa gctcgagggc aggaagctgc
    10381 ggtagaggct ggtcacgatc agcatgaagc gctcgaacca catgccgatc accaccacga
    10441 tcgcgatact gaacagagct atgtggttgg cgcgcacgcg ccgcagccac agcagctgga
    10501 tcgcgatcac gttgcagagg atcgtcgtcc agaagatcca cgaataggcc ccgcccaggc
    10561 gattcagcgt caaggcgatc tcatagcgat cgccaccgta gaaagcgaag aacacttcgg
    10621 tcacatagct gtagtccacg accagcccca ccgcgagcag catgcgggcg agcttgtcca
    10681 ggtgggcttc ggtgatgaag tcggtcagcc cgtagtagcg gcgcagcggg atgccgagca
    10741 cgagcgccat cgcaaagccc gagaacagcg cgcccgcgac gaagaatggc gggaacagcg
    10801 tcgtatgcca gcccggcagc aggccttcgg agaaatccag cgccaccatg ctgtgcaccg
    10861 agaacacgag cggcacggcg agcccggcca gaatcaggct gagggtctcg aagcgccgcc
    10921 agtggcgcgc ttcgccgcgc cagccgagcg ccaacagccc ataaaacacc tgactgccac
    10981 gcgtacgcgc ccgatcgcgc agggttgcca gatcgggcag aagtcccacg tagaagtaca
    11041 gaatcgacac cgacaggtaa gcagtgatcg cgaagaaatc ccacagcagc gagctacgcc
    11101 attgcggcca cagcgccatg cggttcggat acggcgctag ccagtagaag aaccagggtc
    11161 ggcccaggtg caggatcgga aacagacccg agaccgaaac cgcaaagacc gtcatcgctt
    11221 cggcatagcg gttgagggag ctgcgccagg actggcgcgt cagatagagc gcggccgaga
    11281 tgaaggtccc tgccattccg atgccaatcc accagacgta gtcggcgatg gcaaagcccc
    11341 aggccaccgg aatgtcgatg ccccagatgc ccacgccccg caccagtagc acccagattc
    11401 ccgagcccag caacgcggtc agcacgatgg aggcgacgaa ggccagcagc cagcctcgcc
    11461 ccggagctcg cgcgagcacg atgttgctca ccgggtcggt gacggcctgc agatcgagcc
    11521 ccaggggctg cgccgatgcg ttcacgtgtc gtcctcgaag gcgggattcg gattgcgcaa
    11581 cgaacccagg taggttgtct gaggtctcgt attcagctcc tcgagaaggg cgtagttgcg
    11641 ttgcgaggct ttctcgcgac tgactgcggt cgcaggatct gcggtatccc cgaacgtgat
    11701 ggcctttgcc gggcacacgg cctgacacgc cgtcaccacc tcgccatcgg caatccggcg
    11761 cccctgcttg tctgcctcga tgtgcgcgcg ctcgatgcgc tggatgcagt acgtgcattt
    11821 ttccatcaca ccgcgcatcc gcaccgagac ttccggatta cggctcgcct tcagcgtcgg
    11881 cgagctcacg tcggcgtact gaaggaagtt gaagcggcgg accttgtagg gacagttgtt
    11941 cgaacagaac cgtgtgccga cacatcggtt gtacacctgc acattcagcc cttccgagtc
    12001 gtgcacggtg gctcccaccg gacagacgag ctcgcacggc gcatgtccgc agtgcatgca
    12061 cggcaccggc tggtgatgaa ctcgcggcgc tgcgggtgtg ccttcgaaat atcgatccac
    12121 ccggatccag tgcaattctc tcccgcgggc gacctcctgc gctccgaccg gcggaatgtt
    12181 gttctccgcc tggcaggcca gagtgcaggc tccgcagccg atgcaggcat tcaggtcgat
    12241 actcattccc cagcgatatt cgacgcgcgg ccggggcgga tagagactcg gcggagtttt
    12301 cgatgcgccc tgcaggaagg cgggattgtc gcgaaattgc gcgagcgtgc cggtgcggac
    12361 cgggggttcc ccctccattt ggtgatggcg ctgcgtcgtg accagctcga tatgttttgc
    12421 ggtggaggca atcgtgcact ccgcccgcca tgtcgtggag aggccccgca ccctgtaggc
    12481 atcgaagccc aatcgctcac caacatgccc ggctatggtg cggccgtagc ccaacggcgc
    12541 aaccaccgtg ttgtcggcct ggcccggcat gatccacacc ggtgcctgca cctcgcggcc
    12601 ctcggtgcgc agcgagacca cctctccatt gcgcaggcca tggcgagtcg caaaggccgg
    12661 gccgatcaac gccgcgttgc tccaggtgag ttgattggtg ggcttgggca gctcctgcag
    12721 ccacgcattg ttggcataac gaccgtccca gatcgtcggg tcggggcgga agagcaggtc
    12781 gatctgagct tgcggccgcg gtcgtgagag gcgctgcaga ctcgatgtgt tgcctcgagg
    12841 ttcgacggcc ggtggcgcag aattctcgat cacgccctga cgcagagcgg actgccacct
    12901 ggcctcatca ttcagtccca actgatggct ccaggtgttg cgcacgatct cgcgtgcagt
    12961 cgtccgggtc gactcgagca tgaacgccag gatctcgtgc accgatctga cgtcgtagag
    13021 cggatcgatc accggctgca cgatgctggc ggtgccatcg aatgccctca ggtccgacca
    13081 ggattccagc gcgtgggtcc gcggcaggtg ccaggtgcag cgggccgccg tctcatcgcg
    13141 gtacaacccg acgtggatcg agcgacggac gcgagcaagc cgttctgcga acctgacatc
    13201 cgccggggcg gcatacacag gattcacatc cagcatcaac aaggtgtcca cctcgcctgc
    13261 gcccatggat tcggccagcg agcgcaaggc ctcgccgctg ctctcgcgtg gctcgggcgc
    13321 ttcgaagtac tccaccgtct ggccgatgtt gccgagagcc cgattcatca ggatcgccag
    13381 cgcgtggacg agaggcggct gcgaggcccc ggcgatcacc aggcaagcgc cccgattgct
    13441 cagcaggtcc tgcgcaagcg cgcccaggcg gcgcgccgcg gcgccagggc tcccgggcgc
    13501 tgattccccg gtgagcgttg gatgcgagac gcccagacga acggccagag ctgcggcgag
    13561 tgcgctgata tccccagtcg gcaggggcca gcgatgatcg gcattgctac cggtcagtga
    13621 gggtgtggct tccagcacat aaagtcggtt catctttgcc ggagagccca ccggtcggcg
    13681 ccgggcggca aactcacgcg cggcacgcac atgtccgggc atgctggcga ggaagtcggc
    13741 ttcgagtgag acgatcacct cggcgcgatc gaatcgatgc cgtgcccgca agggtctgcc
    13801 tgagaccgcg agcgcacctt ccaaagcgct gtcctcgtcg accgcctcgt actggtgcca
    13861 acgggcacct ggatagcgat gcagcaactc ctcacgctgg gccgcaagag tcggcgattc
    13921 gatccgaccg ctcagaacat gcagcccact gccatcgcga acgtactgcc ggacggccgc
    13981 gatcgcctcg gcctgaaagt catcccagcc ggtgagcacc ccgtgccgca gcggcgcctg
    14041 cgatcgatcg ggatcccaca gctcgaggat cgccgcctga gtcatggggt ccgtcgagcc
    14101 gagactggcg ggatgctcgg ggttgccttc gagcttcgtg ggccgcccct cgtgagtctc
    14161 cacgagaacg ccgatcacat cagccccgac ctgcagcgtc gtggcgtaga agcggggctg
    14221 cgaaggcaac attccttcgg gggtccggat ccacggcacg atctgctccg ccggcggccc
    14281 gctgcaggcg gtgagcccgc ccagcgcgag ggaggcgccc agcaatccga gaaatccgcg
    14341 ccgatccaac accggatcga gcattgcggc ctgcggcacc gcctccttga agcgctgcag
    14401 catctgcggc tgctcgagca gctcctccag ggatcgccag aagccgcccg gcttgccagc
    14461 cagctcgcgg cggatcgcct cgagcgctgc actgcctggg gttggcatca gcggtggcat
    14521 accgagcagt cggtcagccg gcgcttgtcg atgtgataac gggccagcag cgactctccg
    14581 agcgtggact gatcctgcgg gctccagtcc atcgagaaga cctggctccg gggccggaga
    14641 aatttttccg gattgcggtg gcagtcgagg caccagtgca tcgacagcga ggcctggcgc
    14701 gccatcagcg gcatccggtc cactctgccg tgacagctca cacagccaat gcccttggcg
    14761 atgtggatcg agtgatcgaa gtagacgaaa tcaggcaggt cgtagatgcg ctgccaaagt
    14821 aggggtttgc gcgtctgcca gctgcgcacc agcggcgcca gaatcgcctg gtcggtaaat
    14881 aactccgaat gacaggtcat gcaggtcgag atcgggggaa tgccggcgac cgcatcggtc
    14941 tctactgagg cgtgacagta acggcagtcg agcccgacat cgccgacgtg atgcttgtga
    15001 ctgaaaggca ccggttgctc gaccggctcg cccatcgcgt gaggatcggc agtcaatgcg
    15061 cgccaactca acacgctacc cgtaaacagt accgcgccta ccacaagcgt gagcttgagg
    15121 agggtaccga agcgccggga gaaaatctgg gccacaccgc cgcctgcaag ggagggcggc
    15181 cgaacacagc aaatcgcata ccctgcgccg caggaatcct gtcagcggat gctgagtatc
    15241 ttgtgtgtct gcagactcag ccgccagcgc ggatggccca ggcagtactg caccgcgagc
    15301 tcggtgtgtc gcgcacgctc cgggccatcc agcggctgaa ggaagaaatg ctcgaagtgc
    15361 aacgccgcaa atctttccgg ctcggcaccc acctgcggat agaccagctt cagctcgtcg
    15421 ccttgaagca cgcgcaaggt cgcgccagcc ttcgggctga cggtgatcca cagctcaccg
    15481 gccggtggca ggactgtccc gttggtctcg accgcaacct gccagccgct gctgttgaat
    15541 tgagcgagca acggtgcatc gagctgcagg agcggctctc cgcccgtaca aaccacgaac
    15601 ggcagcgccg agccctcgcg cccgcccgcc ggccagtggc gggtcacggc gctgaccagc
    15661 tggccggccg aagcaaactt gcccccgccg ggcccatcgg tgcccacgaa gtcggtgtcg
    15721 caccagcgcg agcagccccc ggggcccttg gcccgatccc gctccaatcc cgtccagagg
    15781 ttgcagcctg cgaagcgcag gaagaccgcc ggacggccgc tgtgggcgcc ctcgccctgg
    15841 agcgtgtagt aaatttcctt gaccgcgtaa gccatgaagc gagtgtgcca gctatctgga
    15901 gcctgccgcg tatctggagc ctgccgcgta tctggaatct gccggcgaac tggagtctac
    15961 cgcttacctg ggctctgtcc gggacagcag cggatcgaga acgccggctt cacggaagcc
    16021 tttcgcgcgc agcacacagg agtcgcatgc gccgcagggc gtaccgtccg cgcccggatc
    16081 gtagcagctg ctggtcagcc catagtccac cccgagggcg aggccgcgct ggatgatctg
    16141 cgccttggtg agctcgatca ggggggcgtg aagcctgaag gattcgccgc tttgcacccc
    16201 tgcccgcgtg gcgaggttcg ccaggcgctc gaaggcagcg atgtactcgg gacggcagtc
    16261 cggatagccg ctgtagtcga gcgcattgac gccgatgaag atgtcctggg cctgaagcgt
    16321 ttccgcccag gccagcgcaa acgagagaaa gaccgtgttg cgggcgggca cataggtgat
    16381 cggaatgcct gcggccatgt cctgctcggg gcggtccttg gggacctcga gatcgtccgt
    16441 gagagccgag ccgccaaact gacgcaggtc gatatcgacg accacatggc ggagcacgcc
    16501 ggcgcggcgc gcgacgcgct gcgcaaatcc gagctccacg atgtgccgct ggccgtagcg
    16561 aaacgacatc gcgcagacgc tgaaaccttg cgactgggcg atcgccagca cggtgctcga
    16621 gtcgagtccg ccgctcaaca gtacgaccgc ttttcgttcc gcgatggcca tgttgcctga
    16681 gggttgttgc tagcgcaggg actgattcag aagatacgcg agccgcacgc cggctttgct
    16741 gagctgctcg gcggtcactg tcgtcgcatc cgtcacgtag gcggacgaga cgttatagag
    16801 gttggccgag ctggccggag gcaaaagccc ataagcgtga tcccgcgcca ccgcgaagga
    16861 ttcccgggcc cagtccgccg ccgatcccgc ccgccatttc gtgcgctgcg ccgccgtgat
    16921 ccccgcgatc agccgctgag cgatcgccgc ctcgttctca cccagtcgtg cgacaaacgc
    16981 cgtgtcccag tcatggtgca ggctgttcgg ggccagtccc ggtgccatca cgatcttgag
    17041 attcccgccc tggtcatgat cgtcgctcgc gtgcagcggc tggtgcacat cgccgacgaa
    17101 atgcagcacg aactgcaggg ccatgaggcg ctccggtgcc gaggttgcgg gagacttcag
    17161 ctcggcgacg aactcatcga tcttgtcgac gacgcagtcg gccgccggcc cgcgcgaggc
    17221 ggccgtccca gaaggcagcg gcggctcgcc gaagcaagcg gctttgagat ctggcccgtc
    17281 aagctcgaga tccacgaaat gccactggcg cgtcgcctcg tagcgcatct tcgtcgtgcc
    17341 gcggtcggag tcgcggtatt tgtcggccca ggttgcctcc tggtcgatgc gatcatcgcg
    17401 tgtcagcgca ctgtgatctg ccgccagaat cgaattgacc cgcgtgcgca ccgccggatc
    17461 cagataacgc tcggcgatca gcccgatgat ttcgtggcct tcatcgcccc aggagtacgc
    17521 gggagtggcg ccgcaagcga aagcggcgca accgagaatg cg
//
LOCUS       DASRHN010000379         7152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1463781, whole genome shotgun sequence.
ACCESSION   DASRHN010000379 DASRHN010000000
VERSION     DASRHN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463781"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="ruvB"
                     /locus_tag="VFB37_15320"
     CDS             complement(<1..659)
                     /gene="ruvB"
                     /locus_tag="VFB37_15320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZO23871.1"
                     /translation="MSPAPVQMTTDRVTSGGASREEEAAERAIRPRRLDEYVGQTPVK
                     TQLQIFVSAARQRNEALDHVLIFGPPGLGKTTLANIIAAELGVGLRQTSGPVLERPGD
                     LAAQLTNLQPRDVLFVDEIHRLAPIVEEVLYPAMEDYQLDIMIGEGPAARSIKLNLPP
                     FTLVGATTRAGLLTSPLRDRFGIVQRLEFYAVEDLERIVRRSASILGVPLDEGGGRRI
                     A"
     gene            complement(670..1254)
                     /gene="ruvA"
                     /locus_tag="VFB37_15325"
     CDS             complement(670..1254)
                     /gene="ruvA"
                     /locus_tag="VFB37_15325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZO23872.1"
                     /translation="MIGSVRGRIASKMPPQLTVETGGLGYEIEAPMSTFYLLPGVGEE
                     VRLLTHLVVREDAHILYGFATEEERRLFRSLIKVSGVGPKIALALLSGITVEAFAQCV
                     QSQNVAALTHIPGIGRKTAERLIVEMRDRLQALDGLTGKSAPASASSAETEAFGALVA
                     LGYRPAEATRLLKAAGPGTHSTEELIRRALQGAV"
     gene            complement(1251..1787)
                     /gene="ruvC"
                     /locus_tag="VFB37_15330"
     CDS             complement(1251..1787)
                     /gene="ruvC"
                     /locus_tag="VFB37_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZO23873.1"
                     /translation="MAANLRILGLDPGSRRTGFGVIECRGPAYTHVAHGCIAVAELAV
                     AQRLRRIFEALQALIIEYGPAEIAVERVFVNRNVDSALKLGQARGAALCAVPQGVPVF
                     EYAPRAIKQALVGSGAAEKLQVAHMIRTLLQLEGKIPPDASDALAIAICHAHSRQIQI
                     LAQPQLSVHRPAPRASET"
     gene            complement(1822..2358)
                     /locus_tag="VFB37_15335"
     CDS             complement(1822..2358)
                     /locus_tag="VFB37_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZO23874.1"
                     /translation="MMRGGAIHAQGAAIRFRCEGYGPGGAAVLMECLTDNRERTVARV
                     RQILQRYGGHIGARGSVDYLFNHVGLLVYPPGTSEERLIQSALEAGAEDVVVSSDGSI
                     EVLADPLEFETVRALLVESGSAPAFAELTERAATPVPLSGDAAVGMLLLLEALVDLDD
                     VQNVYTNAEISDEVLARI"
     gene            2580..4715
                     /locus_tag="VFB37_15340"
     CDS             2580..4715
                     /locus_tag="VFB37_15340"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZO23875.1"
                     /translation="METNQLCPAPIRDSIAIARRALQEASLADSAHLAIGVEAAEVVG
                     ALTEDATLANAVLVRPALANPDITADRLAPLIGTAASGVATALVRLGDLGLPRDWSPA
                     QGLDARQAETLRKMLLAVVSDPRLVLARLAEQLVELRHARDLPPEERERLAVEARAVF
                     APLANRLGVWQLKWELEDLAFRYLEADKYREIAAALNERRADRELYIETLCGELRTQL
                     GKAGVSAEVYGRPKHMYSIYRKMHRKQLAFEQLFDVRAVRVVVSSVPDCYAALGVVHG
                     LWPYIPGEFDDYIATPKGNFYRSIHTAVIGPGGRSVEVQIRTREMHEHAELGVAAHWT
                     YKEGGARDSQYERKIEWVRRLLEPPDRVDADGAGADRDFLERMRTELFEDRVYALTPK
                     GEVVDLPRGATPLDFAYHVHTALGHRCRGAKVNGRIVPLTHALQNGEIVEIISGRQEA
                     PSRDWLAPEQGYLASGRSRSKVRAWFRKQNVADNRAAGRALVERELARIGARPEHFAA
                     VVQALETRDADHLHQLVGEGEITLTQLVQAAARLIEPRFEKPIVRTAKARTDRSGSPV
                     EIEGVGDLPITLARCCSPLRPQSIAGYVTLGRGVTIHRSGCASLERMRSVKPERVLRV
                     EWTAADQVGLAVEIAIEAFDRRGLVRDLTDVLALEKLSIEAMTTTTAREAGSAQVTIG
                     FSVQDLEQLSRVLRRLSSVPNVIHARRLR"
     gene            4961..6667
                     /locus_tag="VFB37_15345"
     CDS             4961..6667
                     /locus_tag="VFB37_15345"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZO23876.1"
                     /translation="MRVIVVDQDSALLEAIARSLRDRYTVDAVTNKGDCLDLLRQNTF
                     EVIVAGERLEDGSGLELLSQCAKRWPSMLRIFAADRPRLQLLKGRLGPFQLFQTLAYP
                     IDPDRLLATLELAEAAQHANADTANIQHVVLGAEVFAGDGEESSPFPPPRPEPRVAPA
                     MELPAPAARSVPPARRPPRASSAPASARFRTSPATAVAAAPELPPPEEVAPPRRNPVR
                     FPPRIQQPSDAVPPVGSSSEDVLAQAAELAREALTPLAIEPHSRKRAIVVIAAALALG
                     LIVVGLKFFGARPASAPAGTVAAVQPSPVAPVAQSGSESAPASGISQTASTGTASAAA
                     AGSASVVTAPAVAAPLPAASETPRPTHHRPPALAATSSSGGTNSPSVLGASSPALPPE
                     TPVGLTMAPRETLPVGNTAAAHSDPLAPAASSGSGEGRGASVPVSATAAAPGPRATAA
                     TQSNAPATTSSNTPAASNSDEPPPVVREAKLIHAVKADYPSAARRDGIEGYVELAVTV
                     SAQGAVTNVTVSRANPPGVFEKAAVTAVRHYRYDPRYVDGLPAEAHLQVHLDFNPASD
                     AR"
     gene            complement(6701..>7152)
                     /locus_tag="VFB37_15350"
     CDS             complement(6701..>7152)
                     /locus_tag="VFB37_15350"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="E3 component of 2-oxoglutarate dehydrogenase
                     complex; catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZO23877.1"
                     /translation="AHKGKEEGMMVADLIAGNFGEVNYKTVPSVIYTAPEIAWVGQTE
                     EQVKVAGRAYKLGVFPFLASGRARAMEQAQGFAKIIAARDDDEILGVHIIGPLAGELI
                     AEAVLAMEYSASSEDLQRTMHAHPTLSEAVHEAALAVDKRGIDSINK"
BASE COUNT         1209 a         2439 c         2293 g         1211 t
ORIGIN      
        1 tgggcgatgc ggcgtccgcc accttcatca agcggcaccc ccaggatgga ggctgagcgc
       61 cggacgattc tctccagatc ctcgactgcg tagaactcga gccgctggac gattccgaag
      121 cgatcccgca gaggcgaggt cagcaggccc gcacgcgtgg tggcgccaac gagcgtgaac
      181 ggtggcagat tcagcttgat ggagcgcgcg gccggtccct caccgatcat gatatcgagc
      241 tggtagtcct ccatcgccgg atagagcacc tcctcgacga tgggggcgag tcgatggatc
      301 tcgtcgacga acagcacatc tcgcggctgc aggttggtca gttgcgcggc caggtcgcct
      361 ggacgctcga gaaccggccc tgaagtctgc cgcagaccca cgccaagctc tgcggcaatg
      421 atgttggcca gcgtcgtctt gccgagaccc gggggaccga agatgagcac gtgatcgagc
      481 gcctcattgc gctggcgcgc ggcgctgaca aagatctgca gttgggtctt gacgggggtc
      541 tggcccacgt attcatcgag tcgccgtggc cggatcgcgc gctcggcggc ctcctcctcc
      601 cggctggcgc ccccactcgt cacccgatct gtcgtcatct gcactggtgc cggggacacg
      661 ctccctcctt cacactgcgc cctggagagc gcgtcggatc agctcttcgg tggaatgtgt
      721 accgggcccg gcagccttca gcaaccgggt tgcctcagcc ggacgatatc cgagggccac
      781 gagggcccca aaggcttccg tctcggcgct ggaggcgctc gcgggagcgc ttttccccgt
      841 gaggccatcg agagcctgga gccgatcgcg catctccacg atgaggcgct cggcggtctt
      901 gcggccgatt ccggggatgt gagtgagcgc ggccacgttc tgactctgta cgcactgtgc
      961 aaaggcctcg accgtgatgc ccgacagcag cgccagcgca atcttcggcc cgacacccga
     1021 gaccttgatc agactcctga acaggcgccg ctcttcctcg gtggcgaagc cgtacaggat
     1081 atgcgcatcc tcacggacca ccaggtgtgt cagcagccgg acttcctcgc cgacgcccgg
     1141 cagcaggtaa aaggtcgaca tcggcgcttc gatctcgtac cccagaccgc ccgtctccac
     1201 tgtgagctgt gggggcatct tggaggcgat gcgtccgcgc accgagccga tcatgtctcg
     1261 ctcgcccgag gcgcgggcct gtgaacactg agctggggct gcgccaggat ctggatctgc
     1321 ctggagtggg catgacagat ggcaatggcc agcgcatcgg aggcatcggg agggatcttc
     1381 ccctcgagct gcagcaaagt tcggatcatg tgcgcgacct ggagcttctc ggccgcgcca
     1441 gatcctacca gggcctgttt gatggcacgc ggcgcatact cgaacaccgg gactccctgc
     1501 gggacagcac acagtgccgc cccgcgcgcc tgcccgagct tcagcgcgct gtccacgttg
     1561 cggttgacga acactctctc cacggcgatc tccgccggcc cgtattctat gatcagggct
     1621 tgcagggcct cgaaaatgcg ccgcaatcgc tgcgccactg cgagctctgc cacggcgatg
     1681 cagccgtgcg ccacgtgcgt ataggccggt ccgcgacact cgatgacgcc gaacccagta
     1741 cggcgcgagc ccggatcgag gcccaggatg cgcaggttgg ccgccatcac cggtgcacca
     1801 ccagtggcgg cggggcgcgg atcaaatgcg cgccaggacc tcgtccgata tctcggcatt
     1861 cgtgtaaacg ttctgtacgt cgtccagatc taccagagcc tcgagcaaaa gcagcatccc
     1921 aacagcggca tcccccgaca gaggcacggg cgttgccgcg cgctccgtga gctccgcaaa
     1981 ggcgggcgca gagccactct ccaccagcag tgcgcgtacg gtctcgaact cgagcggatc
     2041 ggccagcacc tcgatgctgc catccgagct caccacgacg tcctctgcac cggcttcgag
     2101 cgcggattgg atcagccgct cctcgctcgt tccaggcggg tacaccagca gcccgacatg
     2161 attgaagagg tagtccaccg agccgcgggc gccgatgtga ccgccatacc tctgcaggat
     2221 ctgtcgcact cgagcgacgg tccgctcacg gttgtccgtc aggcactcca tcaacacggc
     2281 ggcaccgccc ggcccatatc cctcacagcg gaagcggatc gcagcgccct gcgcatggat
     2341 ggctcctccc cgcatcatgg gtcccggatc aggggacctt gcctgctgca gacctttgcg
     2401 acggcggatg ctaacccact tgctgtgacc tgccatgtgt cgcctggagg aaacgccgaa
     2461 gcgcccagga gtctctaagc ttcggcgccg ccaaggaaaa caggcgggta tgatagccgc
     2521 aaaccgccac cggggacacg ctgccggcgc ccctgcgcga tgtccgagct cgaactgccg
     2581 tggagaccaa tcaactctgt cctgcgccga tccgcgattc catcgcgatc gcgcggcgcg
     2641 cgctccagga agcgagtctg gcggactccg ctcatctggc aatcggggtg gaagctgccg
     2701 aagtcgttgg cgccctgacg gaggacgcga cgctcgcgaa tgccgtgctg gtgaggccgg
     2761 cgctggcaaa tccggatatc accgccgaca ggctggcgcc tctcatcggc actgcagctt
     2821 ccggtgtcgc gaccgctctg gtgcgcttgg gagacctcgg actgccgcgg gactggtcgc
     2881 ccgctcaggg actcgatgcc agacaggccg agacgctgcg caagatgctc ttggcggtcg
     2941 tgagcgatcc gcgtctggtg ctggcgcggc tcgccgagca gctggtggag ctgcgccacg
     3001 ctcgcgatct accgcccgaa gagcgcgagc gccttgctgt cgaagcgcgc gcagtgttcg
     3061 cgccgctcgc gaaccgtctg ggcgtctggc aactcaaatg ggagctcgaa gatctcgcct
     3121 tccgttacct cgaggcggac aaataccgcg agattgccgc ggcactcaat gagcgccgcg
     3181 cggaccgtga gctctatatc gagacgctgt gcggggaatt gcggacacag ctgggcaagg
     3241 ccggcgtgag cgccgaggtg tatggccgcc ccaagcacat gtacagcatc taccgaaaga
     3301 tgcaccgcaa acagctcgcg ttcgagcagc tctttgacgt tcgggccgtg cgggtcgtcg
     3361 tgagctcggt ccccgattgt tacgccgcgc tgggtgtcgt gcacgggctc tggccctaca
     3421 tcccgggtga gtttgatgac tacatcgcga cgccgaaggg caacttctac agatcgatcc
     3481 ataccgcggt gatcggtcca ggcggccgca gcgtggaagt gcagatccgc acccgcgaaa
     3541 tgcacgagca tgccgagctc ggcgtggccg ctcactggac gtacaaggag ggcggtgcac
     3601 gggactcgca atacgagcgc aagatcgaat gggtgcggcg gttgctcgag ccgcccgatc
     3661 gggtggatgc ggacggcgcg ggcgcggacc gtgattttct cgagcgcatg cgcaccgagc
     3721 tgttcgaaga tcgggtctac gccctcaccc cgaagggcga agtggtggat ctgccacgcg
     3781 gggcgacgcc cctcgacttc gcctaccacg tgcacaccgc gctgggccat cgctgccgtg
     3841 gagcgaaggt gaacggccgc atcgttcccc tcacccacgc gctgcagaat ggcgagatcg
     3901 tcgagatcat cagcggcagg caggaagctc cgagtcgcga ctggctcgct cccgagcagg
     3961 gctacctcgc ttccgggcgc agccgctcga aggtgcgggc ctggttccgc aaacagaacg
     4021 tcgcggacaa ccgcgccgca ggacgcgcgc tggtcgagcg cgagctcgca cgcatcggtg
     4081 cccgcccgga gcactttgcc gccgtcgtgc aggcgctcga gacccgcgac gccgatcatc
     4141 tgcatcagct tgttggcgaa ggcgagatta ccctcaccca gctcgtgcag gccgctgcgc
     4201 ggctcatcga gccgcgcttt gagaagccga tcgtgcgtac tgcgaaggca cgcaccgacc
     4261 gcagtggatc tccggttgag atcgaaggcg tcggggatct gcccatcacc cttgcgcgct
     4321 gctgctcgcc tctaaggcca cagtcgatcg ccggttacgt gacgctcggt cgtggtgtca
     4381 ccatccatcg cagcggctgc gccagtctgg agcgcatgcg atcggtcaaa cccgagcgcg
     4441 tcttgcgcgt ggagtggaca gccgcagatc aggtcggtct ggcggtggaa attgcgatcg
     4501 aggccttcga tcgccgcgga ctggtgcgcg atctcacgga tgtcctggca ctcgagaagc
     4561 tcagtatcga ggccatgacg accacgaccg ctcgagaggc aggttccgcc caggtgacga
     4621 tcggcttttc cgtccaggat ctcgagcagc tctccagagt tcttcgcagg ctcagcagcg
     4681 ttccgaatgt gatccatgca cggcggctgc ggtgacttcg ggccgcaggg ggacgcgctc
     4741 cagcccctat tttctgcacc ccgctgtgag cgggccatcc gcaatatgca aaccgtgatc
     4801 tggcactgcg ccgggaacgt cgtccacccg ttaactagcc actacggttt gccggtttaa
     4861 ctgcttgagg ctcaagccat tcctcccggg gagccccctg ccgattcggg gcgttcaacg
     4921 gccatgagga cagggctcga gctggagtgt ctgccgcccc atgcgcgtga ttgtcgtcga
     4981 ccaggactcc gcgttgctcg aggcgatcgc ccgatcgctt cgggaccggt ataccgtcga
     5041 tgcggtaacc aacaaaggcg actgtctcga tctgctgcgg cagaacacct tcgaagtcat
     5101 cgttgccggt gagcgcctcg aggacggctc gggactggag ttgctgagtc agtgtgccaa
     5161 gcgctggccc tccatgttgc gcatcttcgc tgccgaccgg ccgcggctgc agctcctcaa
     5221 gggccggctc ggtcccttcc agctcttcca gacactcgcc tacccgatcg atccggatcg
     5281 gctcctcgcc accctcgagc tcgcagaggc cgcgcagcat gcgaatgctg acacggccaa
     5341 catccagcac gtagtgctgg gcgccgaagt atttgccggg gacggcgagg agtcttcgcc
     5401 attcccgccg cctcggccag aaccgcgtgt cgcaccagcc atggagctgc cggcacctgc
     5461 cgcgcggagc gtaccgccgg ccagacgacc tccgcgcgct tcgagcgcac cggcctcagc
     5521 tcgtttccgg acaagccccg cgacggcagt agccgcagcg cccgagctgc cgccacccga
     5581 agaagtcgca ccgcccaggc gtaacccggt tcgctttccg ccacggattc agcagccgtc
     5641 cgatgcagtc ccgccagtcg gctccagctc tgaagatgtt ttggcccaag cggccgagtt
     5701 ggcgcgtgag gcgctcacac cgctggcgat cgagccgcat tcccgcaagc gagcgatagt
     5761 cgtcattgct gccgccctcg cgttaggttt gatcgtggtc ggtttgaagt tctttggtgc
     5821 tcggcccgcg agtgctccgg ctggcacggt ggccgcggtg caaccgagtc cggtcgctcc
     5881 tgtcgcgcag tcgggctcag agagcgctcc ggcgagcggg atttcgcaga cggcttcaac
     5941 agggacggca agtgcagcag cagcgggcag cgcatctgtc gtgacggcac ctgcggttgc
     6001 cgcgccgctg ccggcggcca gcgagacacc gcgtcccacc catcatcggc ccccggcgct
     6061 tgcggcaacc tcatccagcg gcgggacgaa ttcgccttcg gttttggggg caagctcacc
     6121 ggcgctgcca cccgagacgc ccgtgggatt gacgatggcc cctcgcgaga cgctccctgt
     6181 cggcaacacc gccgccgcac acagcgatcc gctcgccccc gcagcttcat cgggcagcgg
     6241 cgaggggcgc ggtgcgtcgg tgcccgtgag cgccacggct gccgcgccag ggcccagggc
     6301 tacagccgcc acccaatcca acgcgccggc gactacctca tccaacaccc cggcagcttc
     6361 gaattccgat gagccgccgc cggtcgtccg cgaggccaag ctgatccacg cggtgaaggc
     6421 ggactatcct tcggccgcca ggcgcgacgg catcgagggc tacgtcgagc tggccgtgac
     6481 ggtctcggcg caaggcgccg tcaccaacgt cacggtaagt cgcgccaacc cgccaggagt
     6541 tttcgaaaag gccgccgtca cggcggtccg tcactatcgc tacgacccgc gttacgtgga
     6601 tggtctgccc gcagaggcgc acctgcaggt gcacctggat ttcaacccag cctcggatgc
     6661 acgctgagcc cagcgcctgc ttgtcagttg agtcccgcgc ctacttgttg atcgaatcga
     6721 tcccgcgctt atccacagcg agagccgcct cgtgcacggc ctctgacagt gtcgggtgag
     6781 catgcatggt gcgctgcaga tcctcgctgc tggcagagta ttccatggca agaaccgctt
     6841 ctgcgatcag ctcgccggcg agagggccga tgatgtggac gcccaggatc tcatcgtcgt
     6901 ccctcgctgc gatgatctta gcgaagccct gcgcctgctc catggctcgc gcgcggccac
     6961 tggccaggaa gggaaacacg ccgagcttgt aggcacggcc agcgaccttc acctgctcct
     7021 ccgtctgacc cacccaggcg atctcgggag cggtgtagat caccgagggc accgtcttgt
     7081 aattgacttc gccgaagttc ccggcaatca aatccgcgac catcatgccc tcttccttgc
     7141 ccttgtgggc ca
//
LOCUS       DASRHN010000380        11248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_475048, whole genome shotgun sequence.
ACCESSION   DASRHN010000380 DASRHN010000000
VERSION     DASRHN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475048"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFB37_15355"
     CDS             complement(<1..421)
                     /locus_tag="VFB37_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO23878.1"
                     /translation="MELDRYDRAIVRLLQQDARMTNALLAEKVSLSESACLRRVRALE
                     ESGLIQGYTALIDQHKAGYPVNVFVNITLDRQSQSGLEAFENAVRKVPEVMECYLMTG
                     EHDYLLRLVVADLADFERVHNQHLTRLPSVASVQSSFA"
     gene            595..1707
                     /gene="ald"
                     /locus_tag="VFB37_15360"
     CDS             595..1707
                     /gene="ald"
                     /locus_tag="VFB37_15360"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZO23879.1"
                     /translation="MRIGVPKEIKVHEYRVGLVPAGVRELVASAHQVVVEATAGNGIG
                     VDDSHYVAAGASIAPSAAEVFAHSDLIVKVKEPQPVECAMLRSGQVLFTYLHLAADPE
                     QARGLMKSGATAIAYETVTAPNGSLPLLTPMSEVAGRMSIQVGAASLQKANGGFGVLL
                     GGVPGVQPAKVLVLGGGVAGTHAVEMAVGMRADVTVVDRSLDRLRELSSTFGSSLLTA
                     YSTTETIERLVRDADLVIGAVLIAGAAAPKLVTRAMLSTMKRGAVLVDISIDQGGCFE
                     TSRPTTHADPTFVVDGIIHYCVANMPGAVPRTSTFALTNATLPYVRALADLGWQAALK
                     RDPGLAAGLNVHAGEITHAVVARALGFEAQIRRIGE"
     gene            1704..2279
                     /locus_tag="VFB37_15365"
     CDS             1704..2279
                     /locus_tag="VFB37_15365"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZO23880.1"
                     /translation="MKNALPAAVLALVTGSALAAPVKYDVDPNHTHPSFATDHFGGLS
                     VWRGLFDVSSGSIELDKEKATGSVEITVDMSSVDFGLPKLSEHVKSAEMFDVQKYPTA
                     TYKGKLTKFRNGAPTEVQGELTMHGVTKPVNLTIDQFKCMINPMSKKEVCGADAAGTL
                     NRADFGVNYGEKMGFKQEVKLAIQVEAIKAG"
     gene            2299..3105
                     /gene="cysQ"
                     /locus_tag="VFB37_15370"
     CDS             2299..3105
                     /gene="cysQ"
                     /locus_tag="VFB37_15370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZO23881.1"
                     /translation="MSREADPALLESMITAAAEAAEVTRRIYLSGFEVQRKADRTPVT
                     AADHASEALILERLGQVAAEVPVVAEEEVAAGRIPLVGDEFFLVDPLDGTREFVARRG
                     EFTVNIALIRAGFPVLGVVCAPVDGALFAGNVGLGQAFRTRVGEGGSIAERTPIRVRA
                     VPQHGLTVVSSRSHSTPQTDAYLTAYTVAERVSVGSSLKFCLVAAGEADLYPRLGPTM
                     EWDTAAGHAVLEAAGGQVQAPGGVPLRYGKPGFRNSFFVASGALNPRSLP"
     gene            3113..4036
                     /gene="cysD"
                     /locus_tag="VFB37_15375"
     CDS             3113..4036
                     /gene="cysD"
                     /locus_tag="VFB37_15375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZO23882.1"
                     /translation="MNAAAASHAVSIGLRRLETEAIGILREVVAEFRNPVMLYSIGKD
                     SGVMLHLAMKAFYPSKPPFPLLHIDTGWKFREMIRHRDQVAARYGLKLLVHMNPEGLA
                     RGINPISSGSRLHTQVMKTEALKQALDHYGFDAAFGGARRDEEKSRAKERIFSFRSAA
                     HTWDPRNQRPELWQLYNTRIAAGETIRVFPLSNWTELDVWQYTRAENIPIVPLYFAAP
                     RPVVQRDGMLIVRDDERMPLLPGESEELRTVRFRSLGCYPLSAAIPSTATTVDEIIDE
                     MVQARTSERSGRLIDADETASMERKKREGYF"
     gene            4036..5931
                     /gene="cysN"
                     /locus_tag="VFB37_15380"
     CDS             4036..5931
                     /gene="cysN"
                     /locus_tag="VFB37_15380"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HZO23883.1"
                     /translation="MTGLAPHVSVAADGPRGPGRSLLRFLTCGSVDDGKSTLIGRLLY
                     ETRLILDDQLAALALDSRRHGTSGPDIDFALLVDGLEAEREQGITIDVAYRYWATAHR
                     SFIVADTPGHEQYTRNMATGASNSDAAVILMDARKGVLTQTRRHTYICALLGIRHLVL
                     AVNKMDLEGYSAERFHDLVAEYLRFTDSLGFASITAIPVSARFGDNITASSEQMRWYD
                     GPTLLACLERLDVEHDLDDKPFRFPVQWVNRPSADFRGFSGTIVSGRVRSGERLIVAT
                     SGQESTLTRIVTADGDLQEARAGDAVTLTLADAVDVARGDVLARPDSRPEVVDQFAAH
                     LLWMTEEAMLPGRSYLMRIGTRYVPARITSLKHKVDVNTLEHIAAKTLTLNEIGLCNL
                     ATAAPVAFDPYSENRATGAFILVDRFTNATAGAGMITFGLRRATNIHRQSVLIDKAAR
                     LRMQGHRPAILWFTGLSGSGKSTIASVLERELHAHGAHTYMLDGDNLRHGLNRDLGFT
                     DVDRVENIRRAGEVARLFVDAGLIVLCSFISPFRAERRMVRELVDPDEFIEIFIDTPL
                     EECMRRDPKGLYARALQGKIKNFTGLDSPYEVPESAEIVIDTRQGSPDGAAQQIIEIL
                     RERRIVA"
     gene            complement(5967..7070)
                     /locus_tag="VFB37_15385"
     CDS             complement(5967..7070)
                     /locus_tag="VFB37_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO23884.1"
                     /translation="MDEVDVVVIGAGVIGLAVARAAARCGRDTLVLEAAAGFGSETSS
                     RNSEVVHAGIHYPPGSLKARLCVSGRELLYEFCERHGIGHRRCGKLIVAVSESERAQL
                     QALHRNARANGVELEILERSQARALEPALSCVGALHSPLSGIVDTHAYMLALLGQAQH
                     HSATFVFNTRVTAMEVRADGILIGVNEASPTLRARQVINCAGLYAPHVAHLIRGFPAE
                     RIPPAYFVKGSYFALSAHAPFQRLLYPLPTHGGLGIHLTLDLAGRARFGPDAQWVRQP
                     DYEVEPERAALFYAAIRTYWPALPDGALHPAYAGVRPRISAPGAALADFRIEGPEAHG
                     VPGIIHLFGIESPGLTASLAIARHVLQLLERRG"
     gene            7138..9228
                     /locus_tag="VFB37_15390"
     CDS             7138..9228
                     /locus_tag="VFB37_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZO23885.1"
                     /translation="MPAVSYRRDGEIAVLEIDNPPVNAASISVREGLAAMLQQAASDP
                     QVKAIVLSGARGSFLAGADINEIASGLSLKFPTLRDLQKQLEGLTKPVIAAIEGVALG
                     GGFEIALTCHGRVAAASARVGLPEVKLGLLPGAGGTQRFTRLAGPEAALEAITSGTHL
                     SAQRALELGTLDAVATNAVPEAIALARQVVRENRPLSLASERQDKIAAVDPALFAAFR
                     HKLERKARGQLAPWRIVDCIEAACTQPREEAFRLERQYFEECRDSPQRKALMHIFFAE
                     REARRIPEVPSSVQPLAIRSAAVVGAGTMGGGIAMSFANAGIPVRLLEVSQEALERGL
                     GLIRKNYAASVARGSLTQAGMDQALSLISGVRQYEALAEADIVIEAVFEDLKVKRQVL
                     TQLDGVCAPHAILATNTSTLDIDAIAAATSRPQSVIGTHFFSPANIMKLLENVRGTNS
                     SSQTIATVMALGKTLGKVAVLAGNCDGFIGNRMLQFYTGEAEFLLEEGASPEQIDRVI
                     EAFGFAMGPLAVRDLAGNDVGFLIRKSRTIPADERVSPILQRLVEAGRLGQKSGKGFY
                     RYEGRVRSPDPEVMALLENVSRELGITRRAIADEEILDRLLHPLVNEGANIVEQGIAI
                     RASDIDVVYVHGYGFPAYKGGPMFWAQETGFERIVATMRKLCATHGERWRPAPLLERM
                     AASGATWKDLPRAG"
     gene            complement(9229..9696)
                     /locus_tag="VFB37_15395"
     CDS             complement(9229..9696)
                     /locus_tag="VFB37_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO23886.1"
                     /translation="MLRTLDRLDRRILDELQQDARMSNQELARRVGLSPAPCWRRLRR
                     LEEAGFITGYATLLDGTTLGLPILAYALVSLENHHPETVRQFDQLVKERPEVLECHSM
                     SGPNDYLLRIVAASMDAYERFMSAHVLQIPAVRSVNTSFVLRTKKFTTRLPVV"
     gene            9851..10858
                     /locus_tag="VFB37_15400"
                     /pseudo
     CDS             9851..10858
                     /locus_tag="VFB37_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
     gene            11003..>11248
                     /locus_tag="VFB37_15405"
     CDS             11003..>11248
                     /locus_tag="VFB37_15405"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZO23887.1"
                     /translation="MLTAIRRTLDAELATNPRMTVFGEDVGPKGGVHGATLGLQEKYG
                     VGRVFDTSLSEEGIIGRSVGMALAGLLPVPEIQFRKYA"
BASE COUNT         1987 a         3569 c         3737 g         1955 t
ORIGIN      
        1 tcgcgaagct cgactgcacg ctcgccacac tcggcaagcg ggtcaaatgc tggttgtgga
       61 cacgctcgaa gtccgccagg tccgcgacga cgagccgcag cagataatcg tgctcgcccg
      121 tcatcagata acactccatg acctcgggca ccttgcgcac cgcgttctcg aaggcctcga
      181 gcccggactg gctctggcga tccagggtga tgttgacgaa cacattgacc ggatagcccg
      241 ccttgtgctg atcgatgagc gcggtgtagc cctggatcag ccccgactcc tcgagagcac
      301 gcacgcggcg caggcaggcg gattcggaca ggctcacctt ctcggcgagc agggcgttgg
      361 tcatccgcgc gtcctgctgc agcaagcgga cgatggctcg atcgtagcga tccagctcca
      421 ttggcgcaag aatccgtcgt gtcagatggt gttgttcgca gattctgcca catatcgcca
      481 gacctgagaa gatcaacgca aggccctgca ggaccagcct gactaagctg ccgcgataac
      541 aaccagagcg atctcccgtg ccggaaggcg ggaggcgccc gagcaggagc ttccatgcgc
      601 atcggtgtac cgaaagaaat caaggttcat gagtatcggg tcggactggt gcccgcgggg
      661 gtacgagagc tcgtcgcgag cgcccatcag gtcgtggtcg aggccactgc cggcaatggc
      721 atcggcgtgg atgattccca ctatgtcgct gcgggtgcct cgattgcacc cagtgccgcc
      781 gaggtgttcg cgcactccga tctcatcgtc aaggtgaagg agccgcagcc ggtggagtgc
      841 gcgatgctgc gctccggcca ggtgctgttc acctacctgc atctggcggc ggaccccgaa
      901 caggcccggg gtctgatgaa atccggcgcg accgcgatcg cttacgagac cgtgacggcg
      961 ccgaacggct cgctgccact gctcacaccg atgagcgaag tggcgggccg catgtcgatt
     1021 caggtcggcg ccgccagcct gcagaaagcc aacggcggct tcggtgttct gcttggtggt
     1081 gtgcccggcg tgcaacctgc gaaggtgctg gtcctgggag gcggagtagc cggcacccac
     1141 gctgtagaga tggctgtggg catgcgagcg gatgtgaccg tcgtggatcg gtcgctcgac
     1201 aggttgcgcg agctgtcctc gaccttcggc tcgagcctgc tgaccgctta ctcgacgacc
     1261 gagaccatcg agcgcctggt gcgcgatgcg gatctggtca tcggcgcggt gctcattgcc
     1321 ggagcggccg cgcccaagct ggtgacgcgc gccatgctct cgaccatgaa gcgcggagcc
     1381 gtgctcgtgg acatttccat cgaccagggg ggctgcttcg agacgagccg gcctaccacc
     1441 cacgctgatc cgacattcgt ggtcgatgga atcattcact actgtgtagc caacatgccc
     1501 ggcgccgttc ccagaacctc gacgttcgcc ctgacgaatg ccacactgcc ttatgtgcgc
     1561 gcactcgccg atctcggctg gcaggccgcg ctcaagcgcg atccaggtct ggcggccggg
     1621 ttgaacgtgc acgccggcga gatcacccat gccgtggtcg cccgcgcact cggtttcgaa
     1681 gcccagattc ggaggattgg agaatgaaga acgcattgcc cgctgccgtg ctggcgctcg
     1741 tgactggctc ggctttggcc gctccggtga agtacgacgt cgatcccaac cacactcatc
     1801 cgagtttcgc caccgaccac ttcggtggac tttcggtctg gcgcggtttg ttcgatgtct
     1861 cctccgggag catcgagctg gacaaggaga aggcgaccgg cagtgtggag atcaccgtgg
     1921 acatgtcctc ggtcgacttc ggcctcccga agctgagcga gcacgtgaag tcggcggaga
     1981 tgttcgacgt gcagaaatat ccgacggcga cctacaaggg caagctcacg aagttcagga
     2041 acggggcgcc cacggaagtg cagggtgagc tcacgatgca cggcgtgacc aagccggtga
     2101 acctcacgat cgatcagttc aagtgcatga tcaatccgat gtcgaagaag gaagtgtgcg
     2161 gcgcggatgc ggcgggaacc ctcaatcgtg ctgacttcgg cgtcaattac ggcgaaaaga
     2221 tggggttcaa gcaggaagtg aagctcgcca tccaggtgga ggccatcaag gctggttgag
     2281 cgcacacgca cgcgccgcat gtcccgtgaa gccgatcccg ctctgctgga gagcatgatc
     2341 acggcggccg ccgaggcagc cgaggtgact cgcaggatct acctcagcgg cttcgaggtg
     2401 cagcgcaagg cagatcgcac gccggtgacg gccgctgacc acgcatccga ggcactgatc
     2461 ctggagcggc tcggccaagt ggcagcggaa gttcctgtgg tggccgagga ggaggtggcc
     2521 gccgggcgca ttccgctcgt gggcgatgaa ttctttctcg tcgacccgct cgatggcact
     2581 cgggagttcg tcgcgcggcg cggagagttt accgtcaaca tcgcgctcat tcgcgcgggc
     2641 tttcccgtgt tgggggtcgt gtgtgctccg gtcgatggtg ccctcttcgc gggcaacgtg
     2701 ggtttgggcc aggcttttcg gacccgggtg ggcgagggcg gctcaatcgc cgagcgcaca
     2761 ccgattcggg tgcgggccgt gcctcaacac ggactcacgg tggtgagctc gcgatcgcac
     2821 tcgacaccgc agaccgatgc ctatctcacg gcatacaccg tggccgagcg cgtttcagtc
     2881 ggctcatcgc tgaagttctg cctcgtggcc gcgggagagg ccgacctgta tccccgcctc
     2941 gggcccacga tggagtggga tactgcggcg ggccatgcgg tgctcgaggc ggccggcggc
     3001 caggtgcaag cccccggcgg tgtaccgttg cgttacggaa agccgggctt tcgcaactcc
     3061 ttcttcgttg ccagcggcgc gctcaatccc cgctcgttgc cctgagaaat ggatgaacgc
     3121 cgctgctgcc agtcacgctg tctcgatcgg ccttcgacgc ctggaaacgg aggccatcgg
     3181 catcctgcgt gaggtggtag cggaatttcg caaccccgta atgctgtact cgatcgggaa
     3241 ggactccggc gtgatgctgc atctggccat gaaggcgttt tatccttcga agccgccatt
     3301 tccgctgctg cacatcgaca cgggctggaa gttccgcgag atgatccgcc atcgcgatca
     3361 ggtggcggcg cgctatggcc tgaagctgct ggtgcacatg aatcccgagg ggctcgcacg
     3421 cggcatcaac ccgatatcct cgggctcgag gttgcatacc caggtcatga agaccgaggc
     3481 tctgaagcag gcgctcgatc attacggttt cgatgcggct ttcggcggag cccggcggga
     3541 tgaggagaag agccgcgcca aggagcggat attctcgttc cggtcggcgg cgcacacctg
     3601 ggatccacgc aatcagcgac cggagctctg gcagctctac aacacgcgga ttgccgcggg
     3661 ggagaccata cgtgtcttcc cgctttccaa ctggaccgag ctcgatgtct ggcaatacac
     3721 gcgggcggag aacattccca tcgtgccgct ttatttcgcg gccccgcgtc cggtcgtgca
     3781 gcgcgatgga atgctcatcg tgcgggacga cgagagaatg ccgctgttgc cgggggagtc
     3841 cgaggagctg aggacagtgc gcttccgctc gctcggctgc tatccgcttt ccgcagcgat
     3901 ccccagcacg gccaccaccg tcgatgagat catcgatgag atggtgcagg cccgcacctc
     3961 ggagcgttcg ggtcggctca tcgacgcgga cgagaccgcc tcgatggagc gcaagaagcg
     4021 cgaaggttat ttctgatgac cggtctggcg ccacacgtca gtgttgcggc cgatggcccg
     4081 cgtgggccgg gtcgatcgct gctgcggttt ctcacctgcg gctcggtcga tgacggcaag
     4141 tccacactta tcggccggtt gctgtatgag acgcgattga tcctggacga tcagctcgca
     4201 gccctcgcgc tggacagccg ccgccatggc acgagcggcc ccgacatcga tttcgcgtta
     4261 ctcgtggacg ggctggaggc cgagcgagag cagggcatca cgattgatgt ggcctaccgg
     4321 tactgggcca ctgcgcatcg gtcattcatc gtggccgaca cgccgggcca tgagcaatat
     4381 acgcgcaata tggccaccgg cgcctccaac tccgatgcag ccgtcatact catggatgcg
     4441 cgcaagggtg tgctgacgca gacccgccga cacacctaca tctgtgcgtt gctgggtatt
     4501 cgccacctcg tgctcgccgt caacaagatg gatctcgagg gttactccgc agagcgcttt
     4561 cacgacctcg tggccgagta cctgcgcttc acggactcgc tcggcttcgc ttccattacc
     4621 gccatcccgg tctcggcgcg attcggagac aacatcacgg ccagctccga gcaaatgcgc
     4681 tggtacgacg gtcccacgct gctggcttgc ctggaaaggc tcgacgtcga gcacgacctc
     4741 gacgacaagc ccttccgttt tccggtgcaa tgggtcaatc gcccgagcgc cgacttccgt
     4801 ggattctcag ggacgatcgt ctcggggcgg gtgcgcagcg gggagcggct gatcgtggcc
     4861 acttcgggcc aggagtcgac gctgacccga atagtcaccg cggatggcga tctccaggaa
     4921 gcccgcgccg gcgatgcggt gactttgaca ctggccgatg cggtcgatgt cgcgcgcggc
     4981 gatgtcctcg cgcgccccga cagccggccg gaggttgtgg accaattcgc cgcgcatctg
     5041 ctgtggatga ccgaggaggc catgcttccg gggcgctcgt atctgatgcg aatcggcacc
     5101 cgttacgtgc ccgcccgcat cacgagcctc aagcacaagg tggacgtcaa cacgctggag
     5161 cacatcgcgg caaagacact tacgctgaac gagatcggtc tgtgtaatct cgcgaccgca
     5221 gcgcccgtcg ccttcgatcc ctattcggag aaccgcgcca ccggcgcctt catcctggtg
     5281 gaccgcttca cgaatgccac agcgggcgcc ggcatgatca ccttcgggct gcggcgggca
     5341 accaacattc accgtcagag cgtgctgatc gacaaggccg cccgcttgcg catgcagggg
     5401 caccggcccg cgattctctg gttcacgggc ctctccggct cgggcaaaag cacgattgcg
     5461 agcgtgctcg agcgcgagtt gcacgcgcac ggcgctcaca cctacatgct ggacggcgac
     5521 aacctgcgcc acggcttgaa ccgcgacctg ggcttcaccg atgtcgaccg cgtggagaac
     5581 atccgccgcg ccggagaagt cgcacggctg ttcgtggatg cgggcctgat cgtgctctgc
     5641 tcgttcatct caccgtttcg cgccgagcgg cggatggtgc gcgagctggt cgatcccgat
     5701 gagttcatcg agatcttcat cgatacgccg ctcgaggagt gcatgcggcg cgatccgaag
     5761 ggcctgtacg cccgcgcgct gcagggcaag atcaagaact tcaccggcct cgattcgccc
     5821 tacgaggtgc cggagagtgc cgagatcgtc atcgacacgc gccagggctc cccggatggc
     5881 gcggctcagc aaatcatcga gatcctgcgc gagcggcgca tcgtcgccta gcgagccatt
     5941 cgagttcacg ctgaagcgcc gaatgatcaa ccccgccgtt cgagaagctg caggacgtgg
     6001 cgagcgatcg cgagcgatgc ggtgagaccg ggagattcga ttccaaacag gtggatgatg
     6061 cccggtacgc cgtgcgcctc gggtccctcg atcctgaagt cggcgagcgc ggctcccggc
     6121 gccgagatgc gtggcctgac gccggcgtag gcgggatgca aggcgccgtc gggcagggct
     6181 ggccagtaag tgcggatagc cgcataaaaa agcgccgccc gctccggctc gacttcgtaa
     6241 tcgggttgtc tcacccattg cgcgtccgga ccaaaccggg cacggcccgc gagatcgagc
     6301 gtgaggtgaa tacctagccc gccgtgcgtg ggtagcggat agagcagccg ctggaagggt
     6361 gcgtgagcgg acagggcaaa gtagctccct ttgacgaagt aggcaggcgg aatgcgctcg
     6421 gcgggaaatc cgcggatgag atgcgccacg tgcggcgcgt agagcccggc gcaattgatc
     6481 acctgccgcg ctcgcagggt ggggcttgct tcgttcacgc cgatcaatat gccatccgca
     6541 cggacttcca tcgcagtcac gcgggtgttg aagacgaagg tggcggaatg gtgttgcgcc
     6601 tgcccgagga gcgcgagcat gtaggcatgc gtatcgacaa tgccgctgag cggagaatgc
     6661 agcgcgccga cacatgagag cgctggctcg agggcgcgcg cctgggagcg ctcgaggatc
     6721 tcgagctcga cgccattggc acgagcgtta cggtgaagcg cttggagttg cgcgcgctcc
     6781 gattcggata ctgccacgat gagcttcccg caacgccgat ggccgatgcc gtggcgctcg
     6841 cagaactcgt acaacagctc gcggccgctg acgcacaggc gcgccttgag cgatccaggc
     6901 ggatagtgga tgccggcgtg gacgacctcg ctgttgcgcg agctggtctc gctgccgaaa
     6961 cctgccgccg cctccagaac cagcgtgtcg cgaccgcagc gggccgctgc acgcgccacc
     7021 gcgagcccta tgactcccgc gccgatcacg acgacgtcga cctcgtccat cgagacgaag
     7081 tgtagccatt cgcctatcat cgcgggcgcg agccgccgtt ttccgaggag taagcctgtg
     7141 cctgccgtga gctaccgtcg cgatggcgag attgcagtcc tcgaaatcga caatccaccg
     7201 gtcaatgctg ccagcatcag cgtccgcgag ggactggcag cgatgctgca gcaggcggcc
     7261 agcgatccgc aggtcaaggc catcgtcctg tccggggcac gcggctcgtt tctggcgggc
     7321 gcggacatca acgagatcgc atcgggcctg tcgctgaagt ttccgacgct gcgtgatctg
     7381 cagaaacagc tggagggact gacgaagccc gtgatcgcgg ccatcgaggg cgtggcactt
     7441 ggaggcggct ttgaaatcgc gctcacctgc catgggcgag tggcggccgc cagcgcccgg
     7501 gtcggcctgc cggaagtcaa actcgggctg ctgcccggag ccggcggtac gcagcgcttc
     7561 acccgcctgg ccggccccga agcggcgctc gaggcgatta cctccggcac gcatctgtcg
     7621 gcgcaacgcg ccctggagct gggaaccctc gacgctgtgg ctacgaacgc ggtgcccgaa
     7681 gccattgcgc tcgcccgaca ggtcgtgcgg gaaaacagac ccctgagcct tgccagcgag
     7741 cgccaggaca agatcgccgc cgtggatccg gcgctttttg ccgcattccg ccacaagctc
     7801 gagcgcaagg cccgcgggca gctcgcgccc tggcggatcg tggactgcat cgaggcggcc
     7861 tgtacgcagc ctcgcgagga ggccttccgg ctcgaacggc agtacttcga ggaatgccgc
     7921 gacagcccgc agcgcaaggc gctcatgcat atttttttcg cggagcgcga agcacgtcgg
     7981 attcccgagg ttccctcgtc ggtgcagccg ctggcgatcc gcagcgctgc cgttgtcggt
     8041 gccgggacca tgggtggcgg cattgcgatg agctttgcga atgcgggcat tccggtgcgg
     8101 ctgctggagg tttcccagga agcgctcgag cgcggcctcg ggctgattcg caaaaactat
     8161 gcggcttctg tcgcgcgcgg tagcctcact caggcgggca tggatcaggc gctgtcgctc
     8221 atcagcggag tgcgccagta cgaggcgctc gccgaggcgg acatcgtgat cgaggcggtg
     8281 ttcgaggatt tgaaggtcaa gcggcaggtg ttgacgcagc tggatggcgt ctgtgcgccc
     8341 cacgccatcc tcgcaaccaa cacctccaca ctcgacatcg atgcgatcgc cgctgccacg
     8401 agccgcccgc agagcgtgat tggcactcat tttttcagtc cggcaaacat catgaagctg
     8461 ctggagaacg tgcgcgggac gaacagctcc tcgcagacga tcgccaccgt catggccctc
     8521 ggcaagacac tgggcaaggt ggccgtgctc gccggcaact gtgatggctt catcggcaac
     8581 cggatgttgc agttctacac cggcgaggcg gagttcctgc tcgaggaggg cgcgagcccc
     8641 gagcagatcg atcgcgtcat cgaagctttc ggattcgcca tgggcccgct ggcagtacgc
     8701 gatctggccg gaaacgatgt cggctttttg atccgcaaga gccgcacgat acccgccgat
     8761 gagcgggtct cgcccatttt gcagcgtctg gtggaggccg gccggctcgg tcaaaaatcc
     8821 ggcaagggct tctaccggta cgagggccgg gtgcgcagcc ccgaccctga agtcatggcg
     8881 ctcctcgaga acgtgtcgcg ggaactgggc atcacccgcc gcgccattgc cgatgaagag
     8941 atcctcgatc ggctgctgca tccgctggtg aacgaaggcg ccaacatcgt ggaacagggc
     9001 attgccattc gtgccagcga catcgatgtg gtgtatgtgc acgggtacgg ctttcccgct
     9061 tacaagggtg gcccgatgtt ctgggcgcag gagacagggt tcgagcgcat cgtcgccacg
     9121 atgcgcaagc tctgcgcaac gcacggagaa agatggcggc cggcacccct gttggagcgc
     9181 atggcggcaa gcggggcgac ctggaaagat ctgccgcgag caggctgatc agaccaccgg
     9241 caggcgggtg gtgaacttct tcgtgcgcag cacgaagctg gtgttcaccg agcgcaccgc
     9301 cggaatctgc agcacatgcg ccgacatgaa gcgctcataa gcatccatgc tggctgcgac
     9361 gatccgcagc aggtaatcgt tggggccgct catcgagtgg cactcgagta cctcgggccg
     9421 ctccttcacg agctgatcga actgtcgcac ggtctcgggg tggtgattct ccagcgatac
     9481 cagggcgtaa gcgaggatcg gaagcccgag cgtcgtgcca tcgagcagcg ttgcgtagcc
     9541 ggtgatgaag cctgcctcct cgaggcggcg caggcgccgc cagcacggcg ccggcgacag
     9601 cccgacccgg cgcgccagct cctgattgga catgcgcgca tcctgctgca gctcatcgag
     9661 aatgcggcgg tccagacgat ccagggtcct gagcatgagc gtccgccggt ggcaataaga
     9721 ttgctaagat aagcaccgat cgggcaatca gcgcaagctc attgccggac accgggcata
     9781 agctgtgcct cgcgagcttg aaaatacgcc tcttccttgc ggacggaaga gctgaggctc
     9841 aatcggagac atgagcgtgg tggcaccggc tgaatcgata tcgcgaacct cgagcgctgc
     9901 ccgggcgtcc ccgaccgggc cggactggcg gcgcgtgatc actctgctgc aggcctcgcg
     9961 ggcactcgat gacatcgagg aaagccggct gctgccgcag cgcaaagtgc tgtatcagtt
    10021 ttccgcccgc ggacatgact tcgcccagat ccttctcggg ctgcagctca ccgatgcgca
    10081 cgatggtgtc gcgctctatt accgctcgcg accgatcatg ctggctctgg gaaccagcct
    10141 ggaggatgcc gcagccggac cgcttgcgcg caccggctcg ttcagcggag ggcgcgacat
    10201 cggtgtcgtc ttcaacatgc cctcgcaggg cggcgcgacg gtgctgcctg catgcggagg
    10261 cgtgggcacc cagtacacac cggctgccgg ctgggcgcag gccattcgct accggcacga
    10321 ggtcctcggt gaggattcct atcgccacag tatcgcggtc gcgctcggcg gcgatgcctc
    10381 cgttgccacc aatggcttct ggtcggcctt gaccatcgcc accacgcagc gcttgccact
    10441 attgctttac gtcgaggaca acggctacgg catctccgtc gattcccaac accagactcc
    10501 cggaggcaac atcgccgcaa acctccgctc gttccagggt ctgaccctgc tcgaaggcga
    10561 tggaacggat ccccaggagg cctcgagtct gattgcccaa gcggtgcgcc tggtgcgggt
    10621 cgagcgcgct ccagtgctgc tgcgcctcac ggtgccgcgg ctgtccggcc attcaggtca
    10681 ggacacccag acctacaagt ccgctgaaac gctcgcgcag gagcgggctc gggatccgct
    10741 cgcgcgcctg cagcgcttca tggtcccacg actgtgcagt gagtccgact gggggcagct
    10801 cgaggcccgc tcccgtcgtg atgtcgagga ggcaatcgag cgtgcactcg cgcggccgct
    10861 gccggacccc gcggagctcg agcgccacgt gttcagcgag ccgggcgagg gggggttgca
    10921 gattcagggc ggtctggccc ccgagcatca ccatttcccg ccatccacga cgattgccca
    10981 tcctgagggg acccggatca acatgttgac cgcgatccgc cggacgctcg atgcggagct
    11041 ggccaccaat ccgcgcatga cggtgttcgg ggaggacgtg gggcccaagg gcggggtgca
    11101 tggggcgacg ctcggactgc aggaaaagta cggcgtgggg cgcgtctttg acacgagcct
    11161 gtcggaggag ggcatcattg gccgctcggt gggcatggcg ctcgcagggc ttctgccggt
    11221 gcccgagatt cagttccgca agtacgcc
//
LOCUS       DASRHN010000381         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1618246, whole genome shotgun sequence.
ACCESSION   DASRHN010000381 DASRHN010000000
VERSION     DASRHN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618246"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFB37_15410"
     CDS             complement(<1..226)
                     /locus_tag="VFB37_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZO23888.1"
                     /translation="MQRFSYLRAADVYSAARAVQSANAKERPGSETSTQQNAMYVAGG
                     TNVTDYMTLGVLRPSMLVDINRLAAELYWRI"
     gene            complement(229..894)
                     /locus_tag="VFB37_15415"
     CDS             complement(229..894)
                     /locus_tag="VFB37_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZO23889.1"
                     /translation="MSTRDPARFSAGRRSFLRGAASGAASIPLIGGTAAALAQASRAD
                     LDREGTAAQQPAGARPRPDEPETVTLRINRQSHTLAIDPRTTLLDALRDQVGLTGTKK
                     GCGHGQCGACTVHVDGQRILSCMTFAVMLDGREITTIEGLTGPGGQLHPMQQAFIDHD
                     AFQCGYCTPGQIMSAIACVQEGHAGSAAEIREYMSGNLCRCAAYPNIVTAVQQAAGLM
                     KGS"
     gene            complement(1062..3131)
                     /locus_tag="VFB37_15420"
     CDS             complement(1062..3131)
                     /locus_tag="VFB37_15420"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZO23890.1"
                     /translation="MNRLTRRQFLETALALGATAAIAREHARKSSTGWHERRDLYPEG
                     VASGDPEPDSVLLWTRHPFPAGAHGELTVELAQDQDFERVIASTRTRVHAAADWTCRV
                     LVGGLQPAREYWYRFCDSNGLGSRIGRTLTAPAQDDTRPVRFAFISCQNANQGAQHAY
                     RRMIFEDERAPEAERLGFVLHLGDFIYEIVWYPEDRPQGMYDRRLRDIVRYAHGEKIE
                     DFHIPTTLEDYRAIYRAYLHDPDLQDARARFPFVNMWDNHEYSWLGWQGLQDFEGRSR
                     PAQTRKVAANQTWFEYQPARIRKPGGPGLELFEAPQVTDAPITSFDEDGFGQEPNNIA
                     AVQSLTGYRAMRWGRNVELIITDQHSYRSHEPTSRPEAHALSSPDFPELIPQEAVEIL
                     DAGREYASGHAPSSIRLGQTEVPNFRREEPVQTILGRTQKAWFLERLRSSQATWKVWG
                     NTEGTLDFRADPQNLPPGLTRPWPGQGYAGFGGGDYGCAYRERAEIYDFVREHGITGF
                     ATVAGDRHSFWAGLAAKELPPREFEPVGIAFVTGSISAPGIVEALEHKLAKDHPLRAL
                     YLSDRTGGKPAPTINMLLLHGVRACLEFQRTADAVKARRLSNPDLSPHLSFLDLGGHG
                     YATVRASSERFDTEFVCIPRPVERIDRPDGGPLSYRVVHRAQLWRANERPKLERTILE
                     GDPQLSL"
BASE COUNT          547 a         1048 c         1140 g          590 t
ORIGIN      
        1 taatgcgcca atacagctct gcggccagcc ggttgatatc cacgagcatc gaggggcgaa
       61 ggaccccaag ggtcatgtaa tcagtgacgt tggtgccgcc agcgacatac atggcgtttt
      121 gctgcgtgga ggtttcggaa ccgggacgct ccttcgcatt cgcgctctgc accgcacgtg
      181 ctgcggagta cacgtctgcg gcgcgcaggt agctgaaacg ctgcacgctc agctgccctt
      241 catgaggccg gccgcctgct gcacggccgt cacgatgttg gggtaggccg cgcaccggca
      301 cagattgccg ctcatgtatt cgcgaatctc ggcggcagag ccggcatggc cttcctgcac
      361 gcacgcgatg gccgacatga tctgcccggg cgtgcagtag ccgcactgga aggcatcgtg
      421 atcgatgaac gcctgctgca tggggtgcag ttggccaccc ggaccggtga gtccctcgat
      481 ggtcgtgatt tcccggccat cgagcatcac ggcgaacgtc atgcatgaca ggatgcgctg
      541 gccgtccacg tgtacggtgc aggcgccaca ctgaccgtgc ccacatccct tcttggtgcc
      601 cgtgagaccc acctgatcgc gcaatgcatc gagcagcgtt gtgcgtggat cgatcgcgag
      661 ggtgtgggac tggcggttga tgcgcagggt caccgtttcc ggctcatccg gccgcggtcg
      721 agctccggcc ggctgctgag ccgccgtgcc ctcacgatcg agatcggcac ggctggcctg
      781 cgcaagggca gccgccgtcc cgcctatgag aggaatcgag gccgcgccgg aggcggctcc
      841 ccgcagaaag gagcgccgac cagcagagaa tcgggcgggg tctcgcgttg acatgaccgg
      901 atacgcatca gtggtgaagg gtccgtcaac tgaatgcatt ccggatgggg tggttccagg
      961 gaagctgcgg cgaatgggca gcggcgccgt gccgactcac atcgggtccc ggctgtactg
     1021 catgcacttg ttcctgatgg gccagggcat cgagagcgcc gtcacaggct gagctgcggg
     1081 tcgccttcga gaatcgtccg ctcgagcttg ggacgctcat tcgcgcgcca caattgtgcc
     1141 cggtgaacga cccgatatga cagcggccct ccgtcaggcc gatcgattcg ctcgacgggc
     1201 cgtggaatgc agacgaactc ggtgtcgaac cgttcgctgg aggcgcgcac cgtggcatag
     1261 ccgtgtccgc cgaggtccag gaaagacagg tgtggagaga ggtcaggatt tgagagccgg
     1321 cgtgccttga cggcatcagc cgtgcgctga aactccaggc aggcgcgaac accgtgcagc
     1381 agcagcatgt tgatcgtagg ggcgggcttg ccgccggtcc gatccgagag gtacagcgcc
     1441 cgcaaggggt ggtccttcgc cagcttgtgc tccagcgcct cgacgatccc tggcgcggat
     1501 atggagcccg tgacgaaggc gataccgacc ggttcgaact cgcgtggcgg cagctccttg
     1561 gcggcgagcc ccgcccagaa gctgtgccga tcaccggcca ccgtcgcgaa gccggtgatg
     1621 ccgtgctccc gtacgaaatc gtagatctcg gcgcgctcgc gatacgcaca gccgtaatcg
     1681 ccgccgccga acccggcata accctggcca ggccagggtc gcgtcagacc gggcggcagg
     1741 ttctgcgggt cggcacgaaa atcgagcgta ccttcggtgt tgccccagac cttccaggtc
     1801 gcctgcgagc ttcgcaggcg ctcgagaaac caggccttct gcgtgcggcc cagaatcgtc
     1861 tgcacgggct cctcccggcg gaagttcggc acctcggtct ggccgagccg aatgctgctc
     1921 ggtgcatggc cgctcgcgta ttcgcggcct gcatccagta tctcgaccgc ttcctgcggg
     1981 atcagctccg ggaaatcggg gctcgagagc gcatgggcct cggggcggct cgtgggctcg
     2041 tgtgaacggt aactgtgctg gtcagtgatg atgagctcga cattgcgccc ccagcgcatg
     2101 gcgcggtacc ccgtgaggct ctgcacggcg gcgatattgt tgggctcctg cccgaaccca
     2161 tcctcatcga agctcgtaat gggcgcatcg gtgacctgtg gggcctcgaa taactcgagc
     2221 cctggaccac cgggcttacg aatgcgagca ggctggtatt cgaaccaggt ctggttggcc
     2281 gccactttgc gcgtctgcgc aggtctcgag cggccctcga agtcctgcag cccctgccag
     2341 ccgagccagg agtattcgtg gttgtcccac atgttgacga acggaaagcg ggcgcgcgca
     2401 tcctgcagat cggggtcgtg caggtaggcg cgatagatgg cgcggtagtc ctccagcgtc
     2461 gtcggaatgt ggaagtcctc gatcttctcc ccgtgcgcat agcggacgat gtcgcgcaga
     2521 cgacgatcgt acatgccctg cggccgatcc tccgggtacc agacgatctc gtagatgaag
     2581 tccccgagat gcagcacgaa gcccaggcgc tccgcctcgg gggcgcgctc gtcctcgaag
     2641 atcatgcgcc gataggcatg ctgcgcaccc tggttggcgt tctggcagct gatgaaggcg
     2701 aagcgaacgg ggcgcgtatc gtcctgcgct ggagccgtga gcgttctgcc gatgcggctg
     2761 ccgagcccgt tcgaatcgca gaagcggtac cagtactcgc gtgcaggctg taatccgccg
     2821 acgagcactc tgcacgtcca gtcggcggcc gcatggacac gggttcgggt gctggcaatg
     2881 acgcgctcga agtcctggtc ctgcgccagc tccacagtca gctccccgtg ggcgcctgcc
     2941 gggaaggggt ggcgcgtcca cagcaggacg ctgtcgggtt cgggatcgcc agatgcgacg
     3001 ccttctggat acaaatcacg gcgttcatgc cagccggtgc tggacttcct ggcgtgttcg
     3061 cgagcgatcg ctgccgttgc acccagagcg agtgccgtct cgaggaactg gcgtctcgtc
     3121 aaacggttca tgtggcgggt cccggggagg cctacgagct gccgggagct tgccacagcg
     3181 cgcgccgggc ggggccctct tgtcgggggc cgggcccagc gcgtacagcg aagctatctg
     3241 tgaggacggt cctcgtgccg ctcctgcgga tgaggttgct gcggcctcgc ggtctcagta
     3301 tgagcggccg gggcgtgctc gacgg
//
LOCUS       DASRHN010000382        19567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1298933, whole genome shotgun sequence.
ACCESSION   DASRHN010000382 DASRHN010000000
VERSION     DASRHN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19567
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298933"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFB37_15425"
     CDS             <1..1084
                     /locus_tag="VFB37_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZO23891.1"
                     /translation="EDGFRKLPIHWMWWPAIGGLIIGVGGLFEPRALGVGYDVIGTLL
                     AGHATLALIVGILVVKTLIWSLSLGSGTSGGVLAPVFMIGAALGSLLAYVFPPVAPGF
                     WALMSLAAVVGGVMRSPLTGVVFAMELTQQWDALMPLIVASTVAFLLSVLVLKRSVLT
                     EKVVRKGTHLTREYSIDPLEIFLVGEIMPNSFLGFRSGTGLAAAVQAAEATWPTAATQ
                     KLADIQRLYPILDDIDHLVGLVTRQQLLRAGTQLDVNSQAKINDLMVINAVVGYPDMT
                     LRELANLMAERQVSSVPIVDRDNGRLVLGVIALDQVLEARLRDVNEEHKSERVLDWLQ
                     IRALGGGRVLVQSQPDSERGSESESD"
     gene            1300..2118
                     /locus_tag="VFB37_15430"
     CDS             1300..2118
                     /locus_tag="VFB37_15430"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HZO23892.1"
                     /translation="METRNAADMSAWERWIAHAGYVSEGLLYLLIGTFALLETIEGRR
                     RPDGTQGVLIKLSLTPPGELLLAAVALGLASFVAWQLLIAIRDPEHRRGREGRSRRMI
                     RVGHMLSGALHGVIVIEAMRILFGYARTANGERTQKHWIARAFGVPFGRYVVGAVGIG
                     ILIYGFYQWYCALTRGKDRKVDITRTRLRPIMNILATYGLLARGAMFALIGIYLVRAA
                     WGLQARYAIGVAGALGTLRQQPYGEWLLGTVAAGLMTYGLWQIVQEPFRRLPDS"
     gene            complement(2258..3520)
                     /locus_tag="VFB37_15435"
     CDS             complement(2258..3520)
                     /locus_tag="VFB37_15435"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZO23893.1"
                     /translation="MWTILLTVVLTLVVVLIAINLHRPEKEIRHQIEHCHDIADPRFR
                     REMGALLGPAIVGGNRIEALQNGDEIFPSMLRAIAAARQSITFETYIYWSGETGTRFA
                     EALIERARAGVHAHVMLDWVGSEKIDRGLVRRMHSAGVEVERYHPLRWYDAARINNRT
                     HRKVLVCDGKIGFTGGVGIADQWKGHAQDPNHWRDMHFRVEGPVVGQMQAAFLDNWIK
                     TTGNVLHGDAYFPDLEEVGDSDMQLFISSPAGGSTSMRLMYLTAITAAAHSIDIAAAY
                     FIPDRLMTEELIKAARRHVRVRVLVPDRYTDSEVVRIMAKREWGGLLSSGVEIYCYEP
                     TMLHTKMLIFDQFMVSVGSTNFDTRSFELNDEASLNVYDREFARKMTAGFEADLQRSR
                     SYSLEAWRQRGWRERAAELVLLPIRGQL"
     gene            complement(3877..5889)
                     /locus_tag="VFB37_15440"
     CDS             complement(3877..5889)
                     /locus_tag="VFB37_15440"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZO23894.1"
                     /translation="MAETSQAVFLSYASQDAEAAQEICSALRAAGIQVFLDQRELRGG
                     DVWDQKIRREIHDCALFVPVISANTAVRHEGYFRLEWDLADQRTHMMARSRVFILPVC
                     LDTTTEVQADVPESFRRVQWTRLPGGSTPSAFVERVRGLLSPELAPSSAGSVAPVELP
                     QPMRTSRRSKPLLLALVAVVLFAALAYFLARQFWIPKHVRPDRARAVANSSAFSPPPH
                     SIAVLPFVNLSGDASQDYFSDGLTEELLNSLAEINELQVAARTSSFSFKERPDIPAIA
                     HKLNVGAVLEGSVRRSAHTLRITAQLINAVTGFQVWSKTYDRDLGDVLQLQTEIATAV
                     ASALKVTLLGDVAAKVELGGTRDPAAFDAYLRAAKAVNLTHGAKDLPVAVAAYTEAIG
                     LDPHYALAFAGRSIALSEVAAEADTASAARDFDKARTDAGRALDLAPNLAQAHAALAL
                     VSELGTLDFTQASESYRRALALAPGNAEVLRGSGIFAARMGHFDAALAATRRALVLDP
                     LARQTHSAYSRALYAARRYREALVAFAGVMSLDPDFRSTYAERGLTYYGLGDLQSARA
                     SCELKPDFWYSQQCLAVVYDKLGRHADAEAELAKLRASTSDGGAYQCATIYAQWGNRA
                     KALEWLEKALRLRDPGLVNIKTDPLLDSLRKEPRFRAIERELKFPG"
     gene            complement(6227..6301)
                     /locus_tag="VFB37_15445"
     tRNA            complement(6227..6301)
                     /locus_tag="VFB37_15445"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6267..6269),aa:Asn,seq:gtt)
     gene            complement(6395..7594)
                     /locus_tag="VFB37_15450"
     CDS             complement(6395..7594)
                     /locus_tag="VFB37_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZO23895.1"
                     /translation="MSKQDFPRVLCVDDEARVVEGLVLHLRKDYEVHTALSGAEALKK
                     LKEVGGAAVVISDMRMPAMDGATFLHHVMSFYPDATRILLTGEPGRDAAIAAVNKANI
                     FRFLTKPCPPDQLRAAVEAGVMQNRLIRAERTILKETLIGCIQALIDVLSITNPVAFG
                     RASRLKRQAMEFAGTLESGDFWQLEAAAMLSQLGYLSLPTQLVEKLYYGEALTATEKT
                     LARGVPDVAMKLLGNIPRLEPVMQILAALNSNDEQVARLGEGTTALGARILGLVIEYD
                     SLVTQGHAMDVAVQTLRQRSARYGSDLIERFATHLGAGGGGSELREMPLRSVVAGMII
                     MQDVRTQMGTLLVPRGFEVSGTFLERIRNFGPDLLSEVVKVLVPAPKAAPVTTERSAA
                     PAAAAAR"
     gene            complement(7587..8801)
                     /locus_tag="VFB37_15455"
     CDS             complement(7587..8801)
                     /locus_tag="VFB37_15455"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO23896.1"
                     /translation="MKRILFVDDEQAALDGLRYRLHRMASCWSTECVASGESAIERME
                     REPFDVVVTDMRMPGMDGAELLEIVSNRWPQTVRIVLSGYGDLKQTIRLVPFAHQYLS
                     KPCEPRQLEDVINRCFALQDMLNGSDLRALVGRIKKLPALPRIYSALQAMTRDENVTA
                     AEIANLISTDTAIAARVLQIVNSAFFRLARRITNIEQAVSYLGFTAIRNVATSVEIFS
                     QWPSTTSSHLDQEALQAHVRAVAAAASALTSKSRIGDDTMLAGLLHDIGYWVLAQECA
                     ADLNKAVELAASANLPLYEAEIQVMGASHAEVGAYLLGIWGLPYPVIEAVAHHHQPQR
                     VVQSDFDVLAALVIAQSLAPLDDSSAFDVALPADPKVDAAYMAALKAPYSWNEAERRV
                     AETVEKEEVQNE"
     gene            complement(8798..10144)
                     /locus_tag="VFB37_15460"
     CDS             complement(8798..10144)
                     /locus_tag="VFB37_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO23897.1"
                     /translation="MPEPDSLEALLAAERAERHRLQSELELRDSALNSASSHFLILDV
                     TRPAWSIVYANRAVAKDHGYTPAELLGKSPTLLLDLEQHQEPFGRLRQAVREGDSTSV
                     EVISRRRDGSTFCVGMFLTPLRPASGATITHYLAIGADITVRLEQERTRQLLQEQLYN
                     EMKERERIGIELRLAQKLESVGRLAAGIAHEINTPIQYIGDSVSFLQATQMDLAGLRS
                     AYISAMQQMQDSTHARQAMESVRAAERAVDLAFVDQEVPKAFARTLEGVQRVAAIVRA
                     MKEFAHPDSAEQSPADLNRAIETTLLVAHNEYKYCAQVETDLTELPDVMCNVGELNQV
                     FLNLIVNAAHAIAETGKDAREGKITVTSRSLGEQVEISVADNGCGISEENLERIFDPF
                     FTTKPMGRGTGQGLAITRAIVIEKHGGHVNVHSEVGSGTRFVLRLPVDGRQAVRAA"
     gene            complement(10298..11506)
                     /locus_tag="VFB37_15465"
     CDS             complement(10298..11506)
                     /locus_tag="VFB37_15465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZO23898.1"
                     /translation="MTPSVSRRVQRVKPSPTLAVTARAARLKAEGKDVIGLGAGEPDF
                     DTPPHIAQAGIDAIRSGFTRYTNVDGINELKDAISDKFLRDNGLKYERAQILVSSGAK
                     QTVYNLCMAVLDAGDEAIIPAPYWVSYPDMVMLADGLPVMPFAGLAQGYKITPRQLAA
                     AITPKTRLFLLNSPCNPTGAAYTRTELRALGEVLLEHPRIIIGTDDMYEKIYWGQDPF
                     CSLLSAVPELYHRTVTINGVSKAYAMTGWRIGYCGGPRELITAMSTIQGQSTSNASSI
                     SQKAATVALTGDQTCVAHMNEAFKARHDFLVHGLNSLPGVSCLPGCGTFYAFADVSRA
                     IAGLGCRDDHEFAELLLTEGGVAVVPGSGFGAPGHIRLSFACSMQMLEQALARMDRVL
                     ITGRAVAQSA"
     gene            11597..13627
                     /gene="uvrB"
                     /locus_tag="VFB37_15470"
     CDS             11597..13627
                     /gene="uvrB"
                     /locus_tag="VFB37_15470"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZO23899.1"
                     /translation="MEKIPFKLQAGFAPAGDQPEAIEKLVEGLQEGLAHQTLLGVTGS
                     GKTMTVASVIEKVQRPTMVLAHNKTLAAQLYGEFREFFPNNSVEYFVSYYDYYQPEAY
                     VPSSDTYIEKDSSVNEHIEQMRLSATKALLERPDSIIVATVSSIYGLGDPQAYLAMIL
                     HLVRGDRTDQRKLLRRLADMQYTRNELDLTQGTYRVRGDVIDIFPAESEREAVRVELF
                     DDTIESITLFDPLTGAISRKVPRFTVYPGTHYVTPRERLVGAVDLIREDLRTRLRELR
                     DAGKLLEAQRLEQRTRFDMEMLQEVGYCAGIENYSRYLSGRAPGDPPPCLFDYLPRNA
                     LLIIDESHQTIPQLGAMYRGDRSRKEVLVEYGFRLPSALDNRPLKFEEWETLSPQMIF
                     VSATPGRYEAAHAAQTVEQVVRPTGLVDPEVEVRPVGSQVDDVLSEINKRVECNERVL
                     ITTLTKRMAEDLTEYLNEHGVRVRYLHADIETVERTEIIRDLRLGKFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSDNSLIQTIGRAARHINGRAILYADTLTGSMQRAID
                     ETNRRRHKQIEYNREHGITPRGIQKAVADIMEGARAPTPIPTGRKRSGKTGEAAAPLD
                     LRPETIVRQIKKLETEMFKRARNLEFEQAAMLRDEIERLKQIELGLPARAAG"
     gene            13662..15503
                     /locus_tag="VFB37_15475"
     CDS             13662..15503
                     /locus_tag="VFB37_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZO23900.1"
                     /translation="MNGRAQGGSDTRMALLTIVVVVAVLKVAKEVFIPLALAILLTFL
                     LAPLVETLRRWRVNRGLAVIVSMLIAFSLIGGLGNVVFNQFADLAHELPDYQHQLRVN
                     LTHLGGVLRLGVEKTSRAVDQLTKEIDRVAPPEPKQRNVSKVQVVQPTENALETVRDI
                     ISPAVKPFATTLAVIVLVAFMLLRLPDLRERVLMLLGARNLHITTEALTEAASRVSRY
                     LLIQILINGWTGLWVALGLWYLGVPNAGLWGALTLVLRFIPYIGVWLAASIPLALSFA
                     VFDDWRRPVEVFGLFAGLELLNYAALEPWLYGTHTGVSAVALLLAAGFWTWLWGFAGL
                     LLAIPMTVCAAVMGKYIPQLNFLWVLLGDEPVLEPHERLYQRLLSSSPDEADRVMHGA
                     LRAKSMLEVCDSILVPAIALVEEDHERGTLTDERRQKIYEHIGVWVDERLDAMDSMRA
                     RLAGFANAESPAPILCIAASDRADELIAKLFEAVLLERGMNPYVTRAREGLEVELPER
                     TMAIVVSALPPEAVPAARAVCKRLHAVVGTVPVCVGLWCATGDLERSRQRLEAAGAQS
                     VVTTLSECCRMLEIERERTRLGIGQPDPPADTDEPLEGSVSHPVITP"
     gene            15583..15657
                     /locus_tag="VFB37_15480"
     tRNA            15583..15657
                     /locus_tag="VFB37_15480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15615..15617,aa:Val,seq:gac)
     gene            15795..17723
                     /gene="thrS"
                     /locus_tag="VFB37_15485"
     CDS             15795..17723
                     /gene="thrS"
                     /locus_tag="VFB37_15485"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZO23901.1"
                     /translation="MPVVTLPDGSQRTFDQPVSVDEIAAAIGPGLRKAALAGRVDGKL
                     VDTSHVVDRDATVAIVTEKDPAGLEVIRHSTAHLLAQAVKELFPEAQVTIGPVIEDGF
                     YYDFAYKRPFTMEDLAAIEARMQQLAQADQRVTRRVLTRDEAVRFFKRLGEHYKAEII
                     AAIPEKDEISLYGQGDWVDLCRGPHVPSTGKLKAFKLTKVAGAYWRGDSRNEMLQRIY
                     GTAWADRKELEAYLHRLEEAEKRDHRRIGRELDLFHLQEEAPGAVFWHSKGWTVFQTL
                     VAYMRERQQLAGYEEVSAPELMDASLWKQSGHLEKYGENMFLTQTPDERVYAIKPMNC
                     PGHIQIFKHGLRSYRELPLRLAEFGKIHRYEPSGALHGLMRVRAFTMDDAHIFLTEDQ
                     ITAECVAVTRLILDIYRDFGFEDLHIKFADRPPKRVGSDEIWDQAEAALVAASRAAGI
                     EFTLNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFNLPGRLGAYYIDEHSGKRTPV
                     MLHRAIFGSLERFFAILLEHHAGRLPAWLAPVQAVVMNITERQEAYVRKLVESLKNQG
                     FRAEFDLRNEKVGFKIREHTLQRVPYLLVAGDKEVAANTISVRTRSGKDLGSLPEETF
                     AERLRIELESRGRRTLEG"
     gene            17727..18248
                     /gene="infC"
                     /locus_tag="VFB37_15490"
     CDS             17727..18248
                     /gene="infC"
                     /locus_tag="VFB37_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZO23902.1"
                     /translation="MASATKRVRRNEDITAPQLRVIAADGSQAGVMSRFDALQMAQSA
                     ELDLVEVSPTAEPPVVRIMDYGKFLFEANKKAHSAKRKQKQIQVKEVKFRPGTGEGDY
                     QIKLRNLIRFLTEGDKAKVTLRFRGREMVHQDIGRRLLDRVSTDLAGHGVIEQNPLME
                     GKQMVMVFAPKKK"
     gene            18345..18548
                     /gene="rpmI"
                     /locus_tag="VFB37_15495"
     CDS             18345..18548
                     /gene="rpmI"
                     /locus_tag="VFB37_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZO23903.1"
                     /translation="MPKLKTNRAAAKRFRKTAKSFKRYSANRRHNLGHKDRKVKRHAR
                     SGGSSLIHESDVGRVTQLLPHHK"
     gene            18558..18920
                     /gene="rplT"
                     /locus_tag="VFB37_15500"
     CDS             18558..18920
                     /gene="rplT"
                     /locus_tag="VFB37_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZO23904.1"
                     /translation="MARVKRGVTAGRRHKKVLGKAKGYYNARRKVYRAAKQAVIKAGQ
                     YAYRDRRAKKREFRALWITRINAAARLHGLSYSRIINGLLKAGVDFDRKVLADLAVHD
                     AEAFGAIAQQAKTALGMS"
     gene            18930..>19567
                     /locus_tag="VFB37_15505"
     CDS             18930..>19567
                     /locus_tag="VFB37_15505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZO23905.1"
                     /translation="MSDLTRLVEQALGEIAACSDLPALDEVRVRWLGKKGVLTEQLKA
                     LGTLPAAERPAAGQRINDAKQGVQGALDARRTALESAEVDRQLAAGRLDVTLPGRGEE
                     RGGLHPVTKARLRIEQLFRRAGFEVASGPEIEDDFHNFEALNIPPNHPARAMHDTFYF
                     PDGRLLRTHTSPVQIRAMLEHGAPIALIAPGRVYRCDYDMTHSPMFHQLEGLV"
BASE COUNT         3541 a         6000 c         6429 g         3597 t
ORIGIN      
        1 cgaggacgga ttcaggaaac ttcccatcca ctggatgtgg tggccggcga tcggaggcct
       61 gatcattggc gtcggcggcc ttttcgagcc ccgtgccctc ggcgtgggct acgacgtgat
      121 cggtaccttg ctcgcaggtc acgctacgct cgccttgatc gtcggcatcc tggtcgtcaa
      181 aacgctgatc tggagcctgt cgctgggttc cggaacctcc gggggcgtgc tcgcgcctgt
      241 gttcatgatc ggcgcggcgc tgggttcctt gctggcctat gtctttccgc ccgtcgcacc
      301 aggtttctgg gcactgatgt ccttggcggc ggtggtggga ggcgtgatgc gcagtccgct
      361 gaccggagtg gtgttcgcca tggagctcac acagcagtgg gatgccctca tgccgctgat
      421 cgtcgcctcc acggtcgcat ttctcttgtc ggtgctggtg ctgaagcggt cggtgttgac
      481 cgagaaggtc gtccgcaagg gtactcatct caccagggaa tattcgatag atccgctgga
      541 gatctttctg gtcggtgaga tcatgcccaa cagcttcctg ggcttccgca gtggcaccgg
      601 ccttgcggct gccgtgcagg cggcggaggc gacatggccc actgctgcca cgcagaagct
      661 cgcggatatc caaaggctat acccgattct cgacgacatt gaccatctgg tcggattggt
      721 tacccgtcag cagctgctca gggccggtac ccagctcgat gtgaactccc aggcgaagat
      781 caacgatctg atggtgatca atgcagtcgt tggctatccc gacatgactc ttcgtgagct
      841 cgcgaatctg atggccgagc gacaggtttc aagtgtgccg atcgtcgacc gcgacaacgg
      901 gcggctggta ctgggtgtga ttgcgctcga tcaggtgctg gaggcgcggc tccgggacgt
      961 gaacgaggag cacaagtccg agcgggtgct ggattggctc caaatccgtg cgctcggcgg
     1021 tggccgggtt ttagtgcaaa gccagccgga cagtgagcga ggctcggaaa gcgagtcgga
     1081 ttaagcgcat cggtcggcac gaacctggag tagtggttgc catgatgatc gaagtgaacg
     1141 gagctccggt ggagctgatg caaatcgatc atctggtgag gaagcacctg tagcgccgaa
     1201 ccgtcctgca gagcgggcgt ggccgcggcg tcgaagcaac ggcgacgcgg aaccgtgatg
     1261 agaaggcgcc gatcggtacg cacaggtagt ttacgcgcta tggagactcg gaacgccgct
     1321 gacatgagcg catgggagcg ttggatcgcg cacgccggct atgtgtccga aggtttgctc
     1381 tatctactga tcggcacctt cgctctgctc gagacgattg agggccggcg gcgcccggac
     1441 ggaacccagg gcgtcctcat caagctcagc ctgacacctc ccggcgagct gctgcttgcc
     1501 gcggttgcgc tcggcctggc ctcgttcgtg gcttggcagc tgctgatcgc cattcgtgat
     1561 ccggagcatc gccgcggtcg cgaaggccgc tcccgtcgca tgattcgagt cggacatatg
     1621 ctgagcggtg cgctccatgg cgtgatcgtg atcgaggcaa tgcgcatcct gtttggatac
     1681 gcacgcaccg cgaacggcga gcggacgcag aagcattgga ttgcgcgagc attcggtgtg
     1741 cccttcgggc gctacgtcgt gggggctgta gggatcggca tattgattta tggtttctac
     1801 cagtggtatt gcgccctgac gcggggcaaa gacaggaaag tggatatcac gcggacgcga
     1861 ctccgaccga tcatgaacat cctcgcgacc tacggcttgc tcgctcgcgg agcgatgttc
     1921 gcgctgatcg gtatctatct cgtccgtgct gcctggggtc tgcaggcgcg gtacgcgatc
     1981 ggcgttgctg gcgcactcgg cacactgagg cagcagcctt acggggagtg gctgctgggc
     2041 accgtcgctg ccggcctgat gacttatggg ctgtggcaaa tcgtccagga accgttccgg
     2101 cgattgcccg atagctgagg cggtagcggc aggccctggc attgaggttc gcgaggagct
     2161 ctttatcagc gcggccgtgg agctcgggac gcgcggctcg tggggttaag cgcgaagagt
     2221 gcgcaatgac gcggcggcgt ggattgcttt tgatgcctca gagctgaccg cgaatcggaa
     2281 ggagcacgag ttctgccgcc cgttcccgcc agccccgctg acgccaggcc tccaggctgt
     2341 aggatcgtga gcgctgcagg tctgcctcga agcccgcggt cattttccgt gcgaactcgc
     2401 gatcgtacac attgaggctc gcctcgtcgt tgagctcaaa ggagcgggta tcgaagttgg
     2461 tcgatccaac ggaaaccatg aactggtcga agatcagcat cttggtgtgc agcatcgtag
     2521 gctcgtaaca gtagatctcg accccgctgc tcagcaggcc accccactct cgcttggcca
     2581 tgatccgcac cacttccgaa tcggtatatc ggtccggcac cagcacacgg acgcgcacat
     2641 gtcggcgagc cgccttgatc agctcctcgg tcatcagccg gtcggggatg aagtaggcgg
     2701 cagcgatgtc gatggagtgt gcggcggccg tgattgcggt gagatacatc aagcgcatgc
     2761 tggtgctgcc gccggctggc gagctgatga acagctgcat gtcactgtct ccgacctctt
     2821 cgaggtcggg gaagtaagca tccccatgca gaacgttgcc tgtcgtcttg atccagttat
     2881 cgaggaacgc tgcctgcatt tgaccgacga ctggaccctc cacgcgaaaa tgcatgtcgc
     2941 gccagtgatt cgggtcctgg gcatgaccct tccactggtc cgcgatgccg accccgccgg
     3001 taaatccgat tttgccgtcg caaacgagca ccttgcggtg ggtgcggttg ttgattcgag
     3061 ccgcgtcgta ccagcgcagc gggtgatatc gctctacctc caccccggcg ctgtgcatgc
     3121 gacgcacgag acccctatcg atcttctcgc tgccgaccca gtcaagcatt acgtgtgcgt
     3181 gcactcccgc gcgcgcccgc tcgatcaatg cctccgcgaa ccgggtgccg gtctcacccg
     3241 accagtagat gtaggtctcg aaggtgatgg actgccgggc ggccgcgatt gcgcgcagca
     3301 tggaggggaa gatctcatcg ccattctgca gcgcctcgat gcggttgccg ccgacgatgg
     3361 ccgggccgag cagggcaccc atctcgcggc gaaagcgggg atctgcgatg tcgtggcaat
     3421 gctcgatttg atggcggatc tccttctccg gtctgtgcag attgatcgcg atcaggacca
     3481 cgacgagggt cagtacgacg gtgagtaaga tggtccacac gcgcgctgct tcgcattgaa
     3541 cgtgcccagg aatggggtcc aagcgacacg accgactgaa tggtcgagct ggcgggaacg
     3601 atcgacagac gaggggctcc aaatacgatg aagaatcaga gtggagtgat tgttttatca
     3661 gcccaaaagg cacggactcg aacgcgcaag tcaggctctt gtcttcatcg ccgtttgggt
     3721 cttattgcgc gccgttatcc cgaagatatt cgggccaagt gaggcgtaga tggtaagccg
     3781 cacaaaatat gtgctgagca gcggtgagtg atcaggagcg actcgctgtg cttcgtgaag
     3841 ggcgcgatct cgcggcgaga tggcgctggc ctgagatcag cctggaaact tcaactcccg
     3901 ctcgatcgcc ctgaagcgcg gctctttgcg caaggagtcc agcagtgggt cggtcttgat
     3961 gttaaccagg cccggatcac gtagccgcag cgccttctct agccattcga gcgccttggc
     4021 acgattaccc cactgcgcgt agatcgtcgc gcactggtac gcgccgccat cgctggtgga
     4081 tgccctcagt ttcgcaagtt ctgcttccgc atccgcgtgc cgacccagct tgtcgtagac
     4141 cactgcgagg cactgttgac tgtaccagaa gtccggcttg agctcacatg aggcgcgcgc
     4201 gctctgaaga tcgccgaggc cgtaataggt gagcccgcgc tccgcgtatg tcgatctaaa
     4261 gtctgggtca aggctcatga ctcctgcgaa agctacaagc gcctcgcgat atcggcgggc
     4321 tgcatacagt gcccggctgt atgcggaatg agtttgtcgt gcgagtggat cgagcacgag
     4381 ggcccgccgc gttgccgcaa gggctgcatc gaagtgcccc atacgggccg caaatatacc
     4441 gcttcctcgc aatacctcgg cattgcccgg cgcaagagct agagctcgcc ggtacgattc
     4501 gctcgcctgc gtaaaatcca gggtccccag ttccgaaacc agagccaatg cagcgtgggc
     4561 ctgggccaga ttcggtgcaa ggtcgagcgc ccgacctgca tcggtacgcg ctttatcaaa
     4621 gtcacgtgcc gcagaggccg tgtcagcctc cgcggcaact tcggaaagcg cgatcgatcg
     4681 gcctgcgaac gcaagcgcat agtgcggatc gaggccgatc gcttctgtgt acgcggcaac
     4741 cgcgacgggg aggtccttgg caccgtgggt caagttcacc gccttggccg cgcgcagata
     4801 cgcatcgaac gctgccggat cgcgcgtccc acccagctcg actttagctg cgacatcgcc
     4861 gagcagagtt actttgagag cgcttgccac tgcggtggcg atctcggttt gaagttgaag
     4921 cacgtccccc aagtcccgat cataggtctt cgaccacacc tgaaagcccg tgaccgcatt
     4981 gatcagctgc gctgtgatcc ggagggtgtg cgcagagcgc cgtacgcttc cctcgagcac
     5041 tgcgccgacg ttgagcttgt gggcaatcgc gggaatgtcg ggacgctctt tgaaggagaa
     5101 cgaggaagtg cgcgcggcga cctgcagctc gttgatctcc gccagcgagt tgagcaattc
     5161 ctcggtgagg ccgtcggaga aatagtcctg actggcatcg ccgctcaggt tcacgaacgg
     5221 cagcaccgcg atcgagtgcg gcggcggact gaaggccgat gagttcgcga cggcccgcgc
     5281 cctgtcgggg cgcacatgtt tggggatcca gaactgcctg gcaaggaagt acgccagcgc
     5341 cgcgaagagg actacggcga cgagcgcgag cagtagcggc tttgatctcc gggaggtgcg
     5401 catcggctgc ggcagttcca caggagcaac cgaccctgcg ctcgatggcg ccagctcggg
     5461 ggacagcaga ccccttaccc gttcgacgaa ggcggacggc gtgctcccgc cgggcaggcg
     5521 tgtccattgc acgcgccgaa aggactctgg aacatccgct tgcacctcgg tggtcgtgtc
     5581 gaggcacacc ggcaaaatga acactcggct gcgggccatc atgtgggtgc gctgatcggc
     5641 caggtcccac tcgaggcgga agtagccttc atgtcgcaca gccgtgttgg ctgatatgac
     5701 gggaacgaac agcgcgcagt cgtggatttc gcgtcggatc ttctgatccc acacatcccc
     5761 gccacgcagc tcgcgctggt cgagaaatac ctggataccg gccgcgcgca gggctgaaca
     5821 aatctcctgg gccgcctccg catcctggga ggcgtagctc agaaacaccg cctgcgaagt
     5881 ctcagccacg ccccaccgcc cttttgatca cgttggaacg cctgattcta tctttcgatg
     5941 cactcgtgtc atcgcgccga gtcaggctca cagagcctca gatgtccaac acttcgggta
     6001 ccgctgtggg cgtacggttg taccaccaaa cctacccccg ggcccatttc gcctggaata
     6061 gtaccgcgca ggagaatcaa acaatcggtg ccgagaattc ttcgagctcc gagagggtcg
     6121 aaccccgcag cgcgttcgca agcggatcga gcggccacgg gtcagaaggg tgcccgatcc
     6181 gacgtcgaat ctagagcatg aaagacatgt aagctattga atatattggc tccccgggcc
     6241 tgattcgaac aggcgaccaa cggattaaca gtccgacgct ctaccgctga gctaccgggg
     6301 aaccgggcac gaaggcgaga aggcggcgaa tcttgcgcaa cggcccccgg atagtcaagg
     6361 aaggtcggac ccggagcaaa ggctcgcgcg gcagctagcg ggcggccgca gcggcgggcg
     6421 cggcagacct ctcggtggtg accggcgcag ccttcggtgc gggcacgagg accttgacca
     6481 cctcgctgag cagatcgggg ccgaagttgc ggatgcgctc gaggaaggtg cccgagactt
     6541 cgaaaccacg tgggacgagg agcgtgccca tctgagtgcg cacgtcctgc atgatgatca
     6601 tccccgccac gaccgagcgc aggggcatct cgcgcagctc gcttccgccg ccgcctgcgc
     6661 ccagatgagt ggcgaagcgc tcgatcagat cagacccgta acgagcggaa cgctggcgca
     6721 aggtctgcac cgccacatcc atagcgtgcc cctgcgtcac gaggctgtcg tactcgatca
     6781 cgagcccgag aatgcgggcg ccgagcgcgg ttgttccctc gccgagccgg gccacctgct
     6841 cgtcattcga gttgagggcg gcgagaatct gcatcaccgg ctcgaggcgc gggatattgc
     6901 cgagcagctt catggccaca tcgggcacgc cccgcgccag agttttctcg gtcgcggtga
     6961 gcgcctcgcc gtagtagagc ttttccacga gctgcgtcgg cagagacagg tagccaagct
     7021 gcgagagcat ggctgccgcc tcgagctgcc agaaatcacc gctctcgagc gtgccggcga
     7081 actccatcgc ctggcgcttg agacggctgg cgcgaccgaa ggcgaccgga ttggtgatcg
     7141 agagcacatc gatgagggcc tggatgcacc cgatgagggt ctccttgagg atggtgcgct
     7201 cggcccggat caggcgattc tgcatcacac ccgcctccac ggcggcgcgc agctgatcgg
     7261 gcgggcaggg cttggtgagg aagcggaaga tgttggcttt attgactgcc gcgatggcgg
     7321 catcgcgacc gggctcgccg gtcagcagga tccgggtcgc atccggatag aagctcatga
     7381 cgtgatgcag aaaggttgcg ccatccattg cgggcatgcg catgtccgag atcacgaccg
     7441 cagcgccacc cacctccttg agcttcttca gagcctccgc tccgctgagt gccgtatgca
     7501 cttcataatc cttgcgaagg tgcagcacca ggccctcgac gacccgtgct tcgtcatcca
     7561 cgcaaagcac ccgcggaaag tcctgcttac tcattctgaa cctcttcctt ctcgacggtc
     7621 tcggccacgc gccgctcggc ctcgttccag gaatacggcg ccttgagggc cgccatgtag
     7681 gccgcgtcga cctttggatc cgcgggcaat gcgacatcga aggccgaaga atcatctagt
     7741 ggcgcgagcg actgtgcgat gacgagcgcc gcaagaacgt cgaaatccga ctggaccacg
     7801 cgctgcggct ggtgatggtg cgcgactgcc tcgatgaccg ggtagggcag gccccagatg
     7861 cccagcagat aggcgccgac ctcggcgtgc gaggcgccca tgacctgtat ttccgcctcg
     7921 tacagcggaa gattggcgga tgcggccagc tcgacggcct tgttgagatc ggcggcacat
     7981 tcctgcgcga gcacccagta gccgatgtcg tgcagcaatc cggcgagcat cgtgtcatcc
     8041 ccgatccggc tcttcgaggt cagtgcactc gcggcggcgg ccaccgcgcg cacgtgggcc
     8101 tgcagtgcct cctggtcgag gtgcgagctc gtggtggagg gccattgcga gaagatctca
     8161 accgaggtcg ccacgttgcg gatggcggtg aatcccagat agctcacggc ctgctcgatg
     8221 ttggtgatgc ggcgcgccag ccggaagaag gccgagttga cgatctgcag cacccgggcg
     8281 gcaatggccg tatccgtcga gatcaggttc gcaatctccg ccgccgtcac gttttcatcg
     8341 cgcgtcatgg cctgcagcgc ggagtaaatt cgcggcaggg cgggaagctt cttgatgcgc
     8401 ccgacgagtg cccgcagatc cgagccgttc agcatgtcct gcagggcaaa gcagcgattg
     8461 atcacatcct cgagctgccg gggctcgcag ggcttgctca gatactggtg cgcgaagggc
     8521 accagccgta tcgtctgctt gaggtcaccg tagcccgaga gcacgatccg cacggtctgc
     8581 ggccagcgat tgctgacgat ctccagcagc tcggcgccat ccatgccagg catgcgcatg
     8641 tccgtgacca cgacatcgaa gggctcgcgc tccatgcgct cgatggcgct ttcgccgctc
     8701 gcaacgcact cggtgctcca gcagctcgcc atccggtgca gccggtagcg aaggccatcg
     8761 agtgcggcct gctcatcatc gacgaacaga atgcgtttca tgcggcgcgc accgcctgcc
     8821 gaccgtcaac cggcagtcgc agcacgaagc gcgtgccgct tccgacttca ctgtggacgt
     8881 tcacatgtcc tccgtgcttc tcgatgacga tcgcccgcgt gatggccagc ccctggcccg
     8941 tgccgcgccc catgggcttc gtcgtgaaga agggatcgaa gatccgctcg aggttctctt
     9001 cggagatgcc gcagccattg tcggcgacgc tgatttcgac ctgctcgccc agtgatcgcg
     9061 aggtgacggt tatttttccc tcccgagcat ccttgccggt ctcggcgatc gcatgggccg
     9121 cattcacgat gaggttgagg aaaacctggt tgagctcgcc gacgttgcac atcacatccg
     9181 ggagctccgt gagatcggtc tcgacctggg cgcaatactt gtactcgttg tgagcgacca
     9241 gcagcgtcgt ctcgatggcg cgattcagat ccgcgggact ctgctccgca ctgtccggat
     9301 gagcgaattc cttcatcgct cgcacgatcg ccgccacgcg ctgcacacct tccagggtcc
     9361 ttgcgaacgc cttggggacc tcctgatcga cgaaagcgag atcgacggcc ctttccgcgg
     9421 cacgcacgct ctccatcgct tgccgcgcat gggtgctgtc ctgcatctgc tgcatggcgc
     9481 tgatgtaggc actgcgcagt ccggcaagat ccatctgcgt cgcctgtaga aaggagacgc
     9541 tgtcgccgat gtactggatt ggagtgttga tctcgtgagc gatgccggct gcgagacgcc
     9601 cgacggactc gagcttctgg gccaggcgca attcgatgcc gatgcgctcg cgctccttca
     9661 tctcattgta aagctgctcc tgcagcagtt gccgcgtgcg ctcctgttcg agcctgacgg
     9721 tgatgtcggc gccgatggcg agatagtggg tgatcgtcgc gccgctggct ggccgcagcg
     9781 gggtgagaaa catcccgaca cagaacgtgg acccatcacg ccgtcgcgag atgacctcga
     9841 cgctcgtgct gtcgccctcg cgtaccgcct ggcgcaggcg cccaaaaggc tcctggtgct
     9901 gctccagatc caggagcagg gtcggggact tgcccagcaa ctcagccggc gtgtagccat
     9961 gatccttggc caccgcccgg ttggcgtaca cgatgctcca cgccggccgc gtgacatcga
    10021 gaatgagaaa atggctggac gcggagttga gagcgctgtc ccgcagctcc agctcgcttt
    10081 gcagccggtg ccgctcggcg cgctcggcgg cgagcaaggc ttccagcgag tcgggttccg
    10141 gcatgcgcgc tcacgtccat gacctgcgtc cgcccgcttt tacgcgaggg ccgccagcgc
    10201 ggcccgtgcg tgggtagcaa tttaaggcaa atacaggtgc gctgccggaa ggccggtcgg
    10261 cagccggggc ggagccgcct gtcgcagtgc gataggctca ggcgctctgg gccaccgctc
    10321 ggccggtgat cagcacgcga tccatccgcg cgagcgcctg ctcgagcatt tgcatgctgc
    10381 aggcgaagga cagacggata tggccgggcg cgccgaagcc cgaacccggc acgaccgcga
    10441 cgccgccttc ggtcagcaac agctcggcga attcatgatc gtcccggcag ccgagtcccg
    10501 cgatggcccg cgacacatcg gcgaaggcgt agaaggtgcc gcagcccggg aggcaactca
    10561 ctcccggcag ggagttgagg ccgtgaacca ggaaatcgtg gcgcgccttg aacgcctcgt
    10621 tcatgtgcgc cacgcaggtc tgatcgccgg tcagtgcgac ggtcgccgct ttctgcgaaa
    10681 tgctcgaggc attcgaggtg gactgaccct gaatggtgga catggccgtg atgagctcgc
    10741 gcgggccgcc gcagtagccg atgcgccatc cggtcatcgc ataagccttc gacacaccgt
    10801 tgatcgtgac cgtgcgatgg tagagctcgg gcacggcgct caggaggctg cagaagggat
    10861 cctgccccca gtagatcttc tcgtacatat catcggtgcc gatgatgatg cgcgggtgct
    10921 ccagcagaac ttcgccgagt gcgcgcagct ccgtgcgcgt gtacgcagcg cccgtcgggt
    10981 tgcaggggct gttgagaaga aacaggcgcg tcttgggcgt gatggccgcg gcaagctgtc
    11041 gaggcgtgat cttgtagccc tgtgcgagcc cggcgaaggg catgaccggc agcccgtcgg
    11101 cgagcatcac catgtctgga tacgacaccc agtacggggc cggaatgatt gcttcgtcac
    11161 ccgcatccag caccgccatg cacaggttgt acacggtctg cttggcgccc gaggaaacga
    11221 gaatctgcgc gcgctcgtac ttgagaccgt tgtcacgcag gaatttgtcg ctgatcgcat
    11281 ccttcagctc gttgatgcca tcgacattcg tgtagcgcgt aaatccgctg cgaatggcgt
    11341 cgatgcccgc ctgcgcgatg tgtggcggcg tatcgaagtc gggctcgccg gctccgaggc
    11401 caatgacgtc tttgccttcc gccttgaggc gggcagcgcg agcggtgacg gcgagcgtag
    11461 gcgagggctt gacgcgctgg acgcgtcggg atacggatgg ggtcacgtag attcctgtga
    11521 tcgctttaat tcggccggcg cggctatatt acgggatcgc gccgcacctc accattctcg
    11581 cggcgcggcc ccttgcatgg aaaaaattcc gttcaaactg caggccggct ttgcccccgc
    11641 cggcgatcag cccgaggcga tcgagaagct cgttgagggc ctccaggaag ggctggcgca
    11701 tcagacgctg ctcggtgtca ccggcagcgg caagacgatg accgtcgcct ccgtgatcga
    11761 aaaggtgcag cgccccacca tggtgcttgc gcacaacaag accctcgctg cgcagctcta
    11821 tggcgagttc cgcgagttct ttccgaacaa ttcggtcgag tatttcgtct cgtactacga
    11881 ctactaccag ccggaggcct acgtcccgtc ctcggacacc tatatagaga aggactcctc
    11941 ggtgaacgag cacatcgagc agatgcggct ctcggccacc aaggccctgc tggaaaggcc
    12001 cgactcgatc atcgtggcca ccgtctcctc gatttacggc ctcggcgatc cgcaggccta
    12061 tctggcgatg atcctgcacc tggtccgcgg ggatcgcacc gatcagcgca agctcctgcg
    12121 gcggcttgcg gatatgcaat acacccgaaa cgagctcgac ctgacgcagg gcacttatcg
    12181 ggtgcgcggc gatgtgatcg acatcttccc tgccgagtcc gagcgcgaag cggtgcgggt
    12241 ggagctgttc gacgacacga tcgagtcgat caccctgttc gacccgctga ccggtgcgat
    12301 ctcgcgcaag gtgccgcgct tcaccgtcta ccctggcacg cattacgtca ctccgcggga
    12361 gcgattagtc ggggccgtgg atctgatccg ggaggatttg cgcacccggc tgcgggagct
    12421 gcgtgatgcc ggcaagctgc tcgaggccca gagactggag cagcgcaccc gcttcgatat
    12481 ggagatgctg caggaagtgg gctattgcgc cgggatcgag aactactcca ggtacctttc
    12541 cgggcgcgcc ccgggcgatc cgccgccatg cctgttcgac tatctgccgc gcaatgcgct
    12601 gctgatcatc gatgagagcc accagacgat tccgcagctc ggcgccatgt accgtggcga
    12661 ccgctcgcgc aaggaagtgc tggtggaata tggcttccgg ctgccctcgg cgctcgacaa
    12721 ccggccgctg aagttcgagg aatgggagac cctgtcgccg cagatgatct tcgtgtcggc
    12781 aacgccgggc cggtacgagg cggcgcacgc ggcgcagacg gttgagcagg tggtgcgccc
    12841 gacgggactg gtggatcccg aagtcgaggt ccggcccgtg ggcagccagg tggatgatgt
    12901 gctctcggaa atcaacaagc gcgtggagtg caacgagcgc gtgctgatca cgacgctcac
    12961 caagcgcatg gcggaggatt tgacagaata tctgaacgag cacggggtgc gcgtgcgcta
    13021 tctgcacgcc gacatcgaga ccgtggagcg gaccgagatc atccgggatc tgcggctcgg
    13081 caagttcgat gtgctcgtcg ggatcaatct gctgcgcgag ggcctcgata tgccggaggt
    13141 ttcgctggtc gccatcctgg atgcggacaa ggagggcttt ctgcgatcgg acaattcgct
    13201 catccagacg atcggacggg cggcccgcca catcaacggc cgggcgatcc tgtatgcgga
    13261 cacactgacg ggatccatgc agcgcgccat cgatgagacc aaccgccggc ggcacaagca
    13321 gatcgagtac aaccgcgagc acggcatcac gccgcgcggc attcagaagg ctgtggccga
    13381 tatcatggag ggggcgcggg cgccgactcc gatccccacg gggcgcaagc ggtccggcaa
    13441 aacgggcgag gccgcggcgc ccctggattt gcggcccgag acgatcgtcc ggcagatcaa
    13501 gaagctcgag acggagatgt tcaagcgcgc ccgcaatctg gagttcgagc aggcggcgat
    13561 gctgcgcgat gagatcgagc ggctgaagca gatcgagctg gggctgccgg cgcgagcggc
    13621 cggctgagca agcaggaacg attcgtgcgt gcgagccgcc catgaacggc cgagcccaag
    13681 gcggcagcga tacccgcatg gccctcctca ccatcgtggt ggtcgtggcc gttctgaagg
    13741 tggcgaagga ggtgttcatt ccccttgcgc tcgccatcct gctgaccttt ctccttgcgc
    13801 cgctcgtgga gacactgcgc cgttggcgcg tcaatcgcgg gctggccgtg atcgtctcca
    13861 tgttgatcgc cttctcgctg atcggtggac tgggcaacgt cgtgttcaac cagtttgccg
    13921 atctcgccca cgagctgccg gactatcagc atcagctgcg tgtgaatctg acacacctcg
    13981 gcggcgttct gcggctcggc gtcgagaaga ccagtcgcgc ggtggatcag ctcaccaagg
    14041 agatcgaccg ggtggctccg ccagagccca aacagcgcaa cgtctccaag gtgcaggtcg
    14101 tgcagccgac ggaaaacgcg ctcgagacgg tacgcgacat catctctccc gcagtgaagc
    14161 cctttgccac gacgctggcc gtgatcgtgc tcgtcgcctt catgctgttg cgattgcctg
    14221 atttgcgcga gcgggtgctg atgctgctgg gcgcccgcaa tctgcacatc accaccgagg
    14281 ccctgacgga agcggccagc cgggtcagcc gctacctcct gatccagatt ctgatcaacg
    14341 gctggacggg actgtgggta gcgctgggcc tgtggtatct gggcgtcccc aatgccgggc
    14401 tgtggggcgc gctgacgctg gtccttcgtt tcattcccta catcggggtg tggctggcgg
    14461 cctccatccc gctggcgttg tcgtttgccg tgttcgatga ctggaggcgg ccggtggagg
    14521 tgtttggtct gttcgcggga cttgagctgc tgaactatgc ggcactggag ccgtggcttt
    14581 acggcactca caccggggtt tcggccgtgg ccttgttgct ggcggcgggc ttctggacgt
    14641 ggctgtgggg tttcgcggga cttctgttgg ccatcccgat gaccgtgtgc gccgccgtga
    14701 tgggcaaata tattccgcag ctcaacttcc tgtgggtgct actcggggat gagccagtcc
    14761 tggagcccca cgagcggctg tatcaacgcc tgctctcgag cagcccggat gaggcggacc
    14821 gggtgatgca tggggcgctg cgggcgaaat cgatgctgga ggtttgcgac tcgatcctcg
    14881 tgccggcgat tgcgctggtc gaagaggacc atgaacgagg cacgctcacg gatgagagac
    14941 ggcagaaaat ttacgagcac atcggtgtct gggtggacga gaggctcgat gcgatggatt
    15001 cgatgcgcgc gcggctcgcg ggcttcgcca atgcggagtc gcctgcaccg atattgtgca
    15061 tcgcggcgtc cgatcgcgct gatgagctga tcgccaagct cttcgaggcc gtcctgctcg
    15121 agcgcggcat gaatccgtac gtgacgcgcg cgcgcgaggg tctcgaggtc gagcttccag
    15181 agcgaaccat ggcgatcgtg gtctccgcat tgccacccga agcagtgccg gctgcgcgag
    15241 ccgtctgcaa gcgcctgcat gcggtggtcg gcacagtgcc tgtgtgcgtg ggcctgtggt
    15301 gtgccacggg cgatctcgag cgctcccgcc agcgcctgga ggcggccgga gcccagagcg
    15361 tcgtcacgac attgtcagag tgctgccgaa tgctggagat cgagagagag cggactcgcc
    15421 tcggcatcgg gcaacccgat ccccccgccg atacagacga gccgctcgag ggctcggtca
    15481 gccatcccgt gatcaccccg taagtgcttg gcaccgctgg ggttagcttg cttcgatacg
    15541 ggccctcttg tatcgttcgc ggccctcgct gccacaattt ccaggcgcgt agctcagtgg
    15601 tagagcatca ccttgacatg gtggtggtcg gtggttcgat cccactcgcg cctaccagtt
    15661 ttgacggggc gaaagccggc tgcgcggctt tcgccagaat ccgtgttggc gccccgcttg
    15721 gggccggcaa aaggcgtggc ttttgccggt aggagagtcc tgcgccggtg gttatcaagt
    15781 gacgaatgac taacatgcct gtagtgacct tgcctgacgg cagtcagcgc actttcgacc
    15841 agccggtctc ggtggacgag atcgcggccg cgatcggccc aggtttgcgc aaggctgcac
    15901 ttgccgggcg cgtggacggc aagctcgttg atacctcgca tgtcgtggac agggacgcga
    15961 ccgtagccat cgttacggaa aaggaccccg ccggtctcga ggtgattcgt cattccaccg
    16021 cacacctgct cgcgcaggcg gtgaaggagc tgttccccga ggcccaggtg accatcggac
    16081 cggtcatcga ggatggcttc tactacgatt tcgcctacaa gcgtcccttc acgatggagg
    16141 acctcgccgc catcgaggcc aggatgcagc agcttgcgca ggcggaccag agagtgaccc
    16201 gccgtgtgct gacacgcgat gaggcggtcc ggttcttcaa gcggctcggc gagcactaca
    16261 aggcggagat cattgccgcc attccggaga aggacgagat cagcctgtac ggacagggcg
    16321 actgggtcga cctgtgccgc ggcccgcatg tgccttcgac cggcaagctc aaggcgttca
    16381 agctcaccaa ggttgcaggt gcgtactggc gaggcgactc gcgcaacgag atgttgcagc
    16441 ggatctacgg caccgcctgg gccgaccgga aggagctgga ggcctacctg caccggctgg
    16501 aggaggccga gaagcgcgat caccgccgca tcggccgcga gctggatctg tttcacctgc
    16561 aggaggaggc ccccggcgcg gtcttctggc actcaaaggg ctggaccgta tttcagacgc
    16621 ttgtcgccta catgcgtgag cggcagcagc tcgcgggcta tgaggaggtc agtgcgccgg
    16681 agctgatgga tgcgagcctg tggaagcagt cggggcacct ggagaaatac ggcgagaaca
    16741 tgtttctcac ccagaccccc gatgagcgcg tgtacgcgat caagccgatg aactgcccgg
    16801 gacacatcca gatcttcaag cacggcttgc gcagttatcg tgagctgccg ttgcgtctgg
    16861 ccgaattcgg caagatccac cgttacgagc cctcgggcgc gctgcatggc ctgatgcgcg
    16921 tgcgcgcctt cacgatggac gatgcccaca tcttcctgac cgaagatcag atcaccgcgg
    16981 aatgcgtggc ggtgacccgg ctgatcctcg atatctaccg ggacttcggc ttcgaggacc
    17041 tgcacatcaa gttcgccgac cggccgccca agcgcgtcgg ctcggacgag atctgggacc
    17101 aggccgaggc ggcactcgtc gccgcttcgc gcgcggcggg gatcgagttc acgctcaatc
    17161 cgggcgaggg cgccttttat ggcccgaagc tggagttcgt cctgcgcgat gcgatcggcc
    17221 gggactggca gtgcgggacg ctgcaggtgg acttcaacct gcccggcagg cttggtgcct
    17281 actatattga tgaacatagt ggaaagcgca ccccggtcat gctgcaccgg gccatcttcg
    17341 gctccctgga acgcttcttc gcgatcctcc tggagcatca cgccgggcgg ttgccggcct
    17401 ggctggcccc tgttcaggcg gtggtgatga atatcaccga gcgccaggag gcctacgttc
    17461 gtaaactcgt agaaagcctg aaaaatcagg gatttcgcgc cgagttcgac ttgcgcaatg
    17521 aaaaggtggg ctttaagatt cgcgaacaca cgctgcagcg tgtgccgtat cttctggttg
    17581 cgggcgacaa ggaagtggcc gcaaacacca tttcagtgcg cacaagaagc ggtaaggact
    17641 tgggctcgtt acccgaggag acgtttgccg aacgtcttcg catcgagctc gagagccgcg
    17701 ggcgccggac gttggagggt tgaagcatcg ctagtgcaac gaagcgtgtt cggcgcaacg
    17761 aggacatcac cgcgccgcag cttcgcgtga tcgcggccga tggatcgcag gccggggtga
    17821 tgtcgcggtt cgacgcgctg cagatggcgc agtctgccga gctcgatctc gtggaagtgt
    17881 caccgacggc ggagccgccg gtggtacgca tcatggatta tgggaagttt ctgttcgagg
    17941 ccaacaagaa ggcccactct gccaagcgca agcagaaaca gatccaggtc aaggaagtga
    18001 agtttcgtcc cgggacggga gagggcgatt accagatcaa actgcgtaat ctcatccgct
    18061 tcctcaccga gggtgacaag gccaaggtaa ccctgcgttt ccgcggccgg gagatggtgc
    18121 atcaggacat cggccgccgc ctgctcgaca gggtctcgac cgatctcgcc ggtcacggcg
    18181 tgatcgagca gaacccgctc atggaaggca agcagatggt catggtgttc gcaccgaaaa
    18241 agaagtaacg gctctgaagg tccgcatggg accgcaggcc gttcgcccgc gagcgggtgc
    18301 gcgagcaccc tgcgaccggt ggctacagta agaggagact gatcgtgccc aagttgaaaa
    18361 ccaaccgggc cgcggcgaag cgttttcgca agactgcgaa gagcttcaag cggtactcgg
    18421 ccaatcgtcg ccacaacctc ggtcacaagg accgcaaggt caagcgccac gcgcgctccg
    18481 ggggctcgag cctcatccac gaaagtgatg tcgggcgtgt cacccagctg ctcccccatc
    18541 acaagtaaga gatcgacatg gcaagagtga aacgcggtgt gacggccggc cgtcgtcaca
    18601 agaaggtgct tggcaaggcg aagggctact acaacgcgcg ccgcaaggtg tatcgcgccg
    18661 ccaagcaggc agtcatcaag gccggacagt atgcgtaccg ggatcggcgc gccaagaagc
    18721 gcgagttccg cgcattgtgg atcacgcgca tcaacgctgc ggcacgcctg cacggcctgt
    18781 cctacagtcg catcatcaac ggcctgctga aagccggtgt tgatttcgac cgcaaggtcc
    18841 tcgcggacct cgccgtgcac gatgcggagg cctttggcgc gatcgcgcag caggcgaaga
    18901 cggccctggg catgagctga gtcgaggtcg tgagcgacct gactcgactc gttgagcagg
    18961 cgctcgggga gattgctgcc tgcagcgatc tgcccgcgct cgatgaggtg cgcgtccgct
    19021 ggctcggcaa gaagggggtg ctgacggagc aactcaaggc tctgggcacg ctgccggctg
    19081 ccgagcggcc cgctgcaggc cagcgcatca acgatgcgaa gcagggtgtg caaggcgcgc
    19141 tcgatgcacg acgcacggcg ctcgagagcg ccgaggtgga ccggcagctg gcggccggaa
    19201 ggctcgatgt caccttgccg ggccgcggcg aggagcgcgg cgggctgcat ccggtcacca
    19261 aggcgcggct gcgcatcgag cagctcttcc gccgggccgg cttcgaggtc gcctcgggcc
    19321 cggagatcga ggacgatttc cacaacttcg aggcactcaa tattccgccc aatcatccgg
    19381 cgcgggcgat gcacgatacg ttttattttc ccgatgggag gctgctgcgc acgcacacct
    19441 cacctgtgca gatccgcgcc atgctggagc acggcgcgcc gattgcactc attgcgccgg
    19501 gtcgggtgta tcgctgcgac tacgacatga ctcactcgcc gatgttccat cagctcgagg
    19561 gactggt
//
LOCUS       DASRHN010000383         8851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_631733, whole genome shotgun sequence.
ACCESSION   DASRHN010000383 DASRHN010000000
VERSION     DASRHN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631733"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFB37_15510"
     CDS             complement(<1..997)
                     /locus_tag="VFB37_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZO23906.1"
                     /translation="MAATGTARRRDDGEPDALRAVREAMRAATLRPQSEAVAEMLEQL
                     APVRPRLAVARHRATRWVQAARATRRSRPLAESLMEQFPLDSAQGRALMSLAEALLRT
                     PDRVRADQLIAERLSAVRSSGASGSHARLLRTGFALLGLASRLLPDAAAELSGRFSAA
                     KLAKPIVAPIVRSALRGAMRMLGEAFIVGETIDRALLRGRSTPGLALCSFDVLGEGAR
                     TDADAQRYLESYGRAIEALRNQPPGSVHERSSISVKLSALEPRYTPLQGERVHERLVP
                     HMLELARNAARAGIGMTIDAEEADRLDLSLDILEALARDPVTRSWDGLGLAVQAYG"
     gene            1126..1596
                     /locus_tag="VFB37_15515"
     CDS             1126..1596
                     /locus_tag="VFB37_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004406045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZO23907.1"
                     /translation="MERSLDRVDHKLLIRLQEDGRTPVSRLAREVNLTVTPTLERVRR
                     LEAAGYIDGYFARLNPGKLGLGLLAYVEVSLDRTTPDAFDRFKQVMLAHDEVMECHMV
                     AGGFDYLLKVRVTDMESYRRFLGDRIASVRGVQQTHTYFVMEEVKSTHRIAVPE"
     gene            complement(1625..3763)
                     /locus_tag="VFB37_15520"
     CDS             complement(1625..3763)
                     /locus_tag="VFB37_15520"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZO23908.1"
                     /translation="MNSTHPVPEGKPAHTNRLISETSPYLQQHAHNPVDWYAWGEEAF
                     ERARRENKPVHVSIGYAACHWCHQMSHESFDDPDTAHLMNELFVNIKVDREERPDVDR
                     IYQIAQQMLTQRSGGWPLTMFLTPDDRRPFFGGTYFPRDSRYGLPGFKDLLARIAAFY
                     HEHETELRRQNEALMAAFADLTPPPAAPETALSDAALKISRAQLARTFDSHNGGFGGA
                     PKFPHPKTLERLLRDWHASAHLTEPDLQALYMATLTLRRMAEGGINDQLGGGFSRYSV
                     DEQWSIPHFEKMLYDNGALLAVYADAAVATGDAFYAEVASRTADWAIREMRSPEGGFY
                     SSFDADSEGHEGKFYVWDRVEVQELLEAPQYRAFAARFGLEEPPNFEGRWHLRISASI
                     DEVADKLALSPGEIQSVLESARRKLLMVRSARVWPGRDEKILTSWNALMIRGLAIAAR
                     ALARDDLAEYAGEALNFVRRTLWSSGRLLATCKDGRAHLNAYLDDYVFLADAVLELQQ
                     VRFRADELGFASELLDVVLEHFADGGSNGFFFTSDDHEPLIHRPKSFSDDATPAGNAI
                     AAFVLQRMGYLLGETRYLSAAEHTLRAGWLAMERFPQAHTSLLNALEEWLNPPEIVII
                     RGPLEPIMMWARELHKLYAPRRLVLAIPADLEGLPQALADKMPGSKLAAPGAKGALAY
                     ICRGSTCSAPVGSLSELITQLRPGAPPAER"
     gene            3943..5166
                     /locus_tag="VFB37_15525"
     CDS             3943..5166
                     /locus_tag="VFB37_15525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZO23909.1"
                     /translation="MTSSSAPALEHASLPVLRLKRGEERRIAAGHMWVFSNEVDTAST
                     PLTALAPGALAQMHSHRGQFLGYAYVNPHALICARIVGRDIDQPLDRALIAQRLETAL
                     ALRRRLNPEPYGRLVFGESDALPGLILDRYGDVVVGQIATAGMEALKADIVAVTRALL
                     NPSTFFWKNDSGARDLEHLPSSAETAFGEIPDTIDVLESGLCFTAPLARGQKTGWFYD
                     QTANRQRLLRYMWPGVRVLDVCSYVGAWAVMALKHGAVSARCVDVSETALQYAARNAA
                     NNGVEVQTIREDAFDALKALQEQHARFDVVILDPPAFIKRKKDIPQGQAAYRKLNQLA
                     LGLLEREGLLVSCSCSYHLAPEDLVQAIRAAARHTDRFVQILEAGGQSPDHPVHPAIP
                     ETRYLKAFFCRVTRE"
     gene            5195..5995
                     /gene="lgt"
                     /locus_tag="VFB37_15530"
     CDS             5195..5995
                     /gene="lgt"
                     /locus_tag="VFB37_15530"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZO23910.1"
                     /translation="MIQYHALDPIAVKIGPLRVHWYGVMYLLGFGAAWLLARLRARKP
                     GSTWRPTDVDDLIFFTMLGVILGGRIGYVLFYGLGFWAKDPLYPLRIWEGGMSFHGGM
                     LGVIAAVSLFAWRRRRNVADVLDFTVPLPGLGLFFGRIGNFINGELWGKPTTVPWGFN
                     VNGEVRHASQLYEAFFEGLVLFAILWWFTSRPRPRLAPSGLFLVIYGIVRFCIEFVRV
                     PDEQIGYLAGGWLTEGQVLSAPMIIGGLALLAYAYRVRTPSGNFSAAQ"
     gene            5992..6786
                     /gene="thyA"
                     /locus_tag="VFB37_15535"
     CDS             5992..6786
                     /gene="thyA"
                     /locus_tag="VFB37_15535"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZO23911.1"
                     /translation="MKQYLELMRHIRESGLRKADRTGTGTLSVFGYQMRFDLARGFPV
                     VTTKRIHLRSLIYELLWFLRGDTNVAWLHEHGVTIWDEWANEGGDLGPVYGKQWRAWQ
                     AADGRTIDQISRLIEQLRSNPNSRRLIVSAWNVGELEKMALLPCHALFQFHVAAGRLS
                     CQLYQRSADVFLGVPFNIASYALLVHMLAQQCELEPGELIWTGGDCHLYLNHLEQADL
                     QLARAPLELPRLVLRRRPPSIFEYEFEDFEIVNYQHHPAIKAPVAI"
     gene            6789..7538
                     /locus_tag="VFB37_15540"
     CDS             6789..7538
                     /locus_tag="VFB37_15540"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZO23912.1"
                     /translation="MADGQDDIAGMLEVRRSPVHGLGAFATRRIEKGARVIEYLGERV
                     SHEEADRRYEYKDAADSHTFLFIVDAGTVIDAGVNGNDARFLNHSCQPNCESVIEGGR
                     VFIEALVEIEPGTELTYDYQIQREADDPDDVDEVFACHCGFPACRGSMLWPPRHEAGA
                     TRSRGRRKHTASGTQPRTRKEARSGKDTRSRTDAGSRMDTRRLKDTRTPKDVRSGKDA
                     RSHKDARRNGPPHRKSAAAKRPSSRRASRRR"
     gene            7553..>8851
                     /locus_tag="VFB37_15545"
     CDS             7553..>8851
                     /locus_tag="VFB37_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZO23913.1"
                     /translation="MQPQRRWSLPAIVGATGIVYGDIGTSPLYAFQTALDAIGTFEPR
                     AILGVLSLIFWSLLLSVTLKYVTVMMRADNEGEGGILALFALAQRKLVVASSWSKAVI
                     GLALAGTAFFFCDALITPAISVLSAVEGLEVLDPDFSRGVVPVTLVVLAMLFLIQKRG
                     SAHVGRLFGPVMIAWFLVIAITGLVAIVRHPTVLAAMDPRYGIWLLVSHSTIALAIIG
                     AVFLALTGGEALYADMGHFGKAPVRIAWFALVWPSLVLNYFGQGALLLERGKLVPESL
                     YALVPASLLPWMVLLATAATVIASQAVISGAFSVARQAVQLDLLPRIRVLQTSALEQA
                     QIYVPMVNMLLFLAVVAFVVGFGSSSALSGAYGAAVVGTMFITDILGAFVAVTQWQWS
                     RWSVAAVFVPLTAMDSVFVAGNMTKVSTGGWIPLTLGALLF"
BASE COUNT         1512 a         2819 c         2835 g         1685 t
ORIGIN      
        1 ggccataagc ctgcaccgcg aggccgaggc catcccagga gcgcgtcacc gggtcccggg
       61 ccagagcctc gaggatatcg agtgaaagat cgagccgatc ggcttcttcg gcatcgatcg
      121 tcatgcctat gcccgcgcga gcagcgttgc gcgccagctc gagcatatgg ggcacgagtc
      181 gctcgtgaac ccgttcacct tgcagagggg tgtagcgtgg ctcgagagcc gacagtttca
      241 ccgagatgct ggagcgctcg tgcacggatc ccggcggctg attgcgaagc gcttctatcg
      301 cacggccgta ggactccaga tagcgctgcg catcggcgtc ggtgcgcgct ccctcaccta
      361 gaacgtcaaa cgagcacagc gcgagccccg gagtcgagcg gccgcggagc agcgcacgat
      421 cgatcgtctc gccaacgatg aacgcctcac cgagcattcg catggctccg cgcaaggccg
      481 agcgaacgat gggtgcgacg attggcttgg cgagcttcgc cgccgagaaa cgtcccgaca
      541 gctccgcggc ggcatccggc agcagacggc tcgcgagtcc gagcagcgcg aacccggtgc
      601 gcagcaggcg ggcatggctg cccgaggcgc cggagctgcg gacagcggac agccgctcgg
      661 caatcaactg gtcggcgcgg actcgatccg gcgtgcgcag cagcgcttcc gcgaggctca
      721 ttaatgcccg gccctgtgcg ctatcgagcg gaaactgctc catcagcgat tccgcgagcg
      781 gccgcgatcg ccgcgtggcg cgcgcggcct gcacccagcg cgtggcacgg tgtctggcaa
      841 ccgcgagcct gggacgcacc ggtgcgagct gttcgagcat ctcggccacg gcttcgctct
      901 gcgggcgcag tgtcgcggca cgcatcgctt cgcgcactgc acgaagtgca tccggttcgc
      961 cgtcgtcgcg ccgccgcgca gtccctgttg cagccatgat cagtttgctc tgttctgctt
     1021 cgcagtcgaa aggatattgt gcttatgata gaacgagaca agcagataat gatctgccaa
     1081 tacagataat ggcagtcgat ttaactatta cggaggcgcc tcacaatgga gcgcagcctg
     1141 gaccgcgtcg atcataagct tttgattaga ttgcaggagg acggccgcac gcccgtgtcc
     1201 cgcctggcac gggaagtgaa tttgactgtc acgcccaccc tggagcgcgt gcgccggctg
     1261 gaggcggccg gatacatcga cggatatttc gcccggctga atcccggcaa gctcggcctg
     1321 ggactgctgg cctatgtgga ggtctcgctc gatcgcacca cgcccgatgc tttcgatcgc
     1381 ttcaagcaag tcatgctggc acacgatgag gtcatggaat gccacatggt ggctggcggc
     1441 ttcgactacc tgctcaaggt ccgtgtgacc gacatggaaa gctatcggcg cttcctcggc
     1501 gacagaatcg catccgtgcg cggagttcag cagacccaca cgtatttcgt gatggaggag
     1561 gtgaagtcca cacatcggat cgcggtgccg gaatgagcgc accgccggat tcctggcggg
     1621 cgactcaccg ctcagccggc ggcgccccag gtctcaactg cgtaatcagt tcactcagcg
     1681 acccgacggg tgccgagcac gtcgaaccac gacagatgta ggcgagggct cccttggctc
     1741 ccggcgctgc gagcttgctg cctggcattt tgtcagcgag ggcttgcggc agaccctcca
     1801 gatcggcggg aatcgcgagc acgagacgtc gcggggcata gagtttgtgc agctctcggg
     1861 cccacatcat gatcggctcc agagggccgc gaatgatgac gatctcaggc ggattcaacc
     1921 attcctcgag cgcgttcagc aggctcgtat gagcttgcgg aaatctctcc atcgccagcc
     1981 agcccgctcg cagtgtgtgc tcggctgcac tcaggtagcg tgtttctccg agcagatagc
     2041 ccattcgctg cagaacgaat gccgcgatcg cattcccggc tggcgtcgca tcgtcgctga
     2101 atgatttcgg acggtggatg agcggctcat gatcatcgga ggtgaagaag aatccattcg
     2161 agccgccatc ggcaaagtgc tccagcacga cgtccagcag ctccgaggcg aagcccagct
     2221 cgtcagctct gaagcgcacc tgttgcagct cgagtaccgc atctgcgagg aaaacgtagt
     2281 catccagata ggcattgagg tgtgctcggc catccttgca ggtcgccagc agccgccccg
     2341 agctccagag ggtccggcgc acgaagttga gtgcctcgcc ggcgtattcc gccagatcgt
     2401 cccgcgccag agcgcgcgcg gcgatggcaa gacctcggat catgagggcg ttccaggagg
     2461 tgaggatttt ctcatcacgc ccgggccata cccgagcgct gcgaaccatc agcagcttcc
     2521 tgcgtgcgga ctccagcacc gactggattt cacccggtga cagggcgagc ttgtccgcaa
     2581 cctcgtcgat cgaagcgctg atgcgcaaat gccaccgacc ttcgaaattg ggaggctctt
     2641 ccaggccaaa gcgcgccgca aacgcgcggt actggggcgc ctccagcagc tcctgcacct
     2701 cgactcgatc ccaaacatag aacttgcctt cgtggccctc ggagtcggca tcgaagctgg
     2761 agtagaaccc tccctccggc gagcgcatct cgcggatcgc ccagtcggcg gtacgtgagg
     2821 cgacctcggc atagaaagca tcgccggtcg cgaccgccgc atcggcatag acggcgagca
     2881 gcgctccatt gtcgtagagc atcttttcga agtgcggaat gctccattgc tcatcgaccg
     2941 agtagcggct gaagccgccg ccgagctgat cgttgatgcc gccttcggcc atccgacgca
     3001 acgtcaaggt agccatgtac aaagcctgca gatccggctc ggtgaggtgc gcgctcgcat
     3061 gccagtcccg caacagccgt tcgagtgtct tcgggtgcgg gaatttcggt gcgcccccga
     3121 agcctccgtt gtgactgtcg aacgtccggg cgagctgcgc acgcgagatt ttcagcgccg
     3181 catcggacag agccgtctcg ggcgccgcag gtggcggggt cagatccgcg aaagcggcca
     3241 tcagcgcctc gttctggcgc cgcaattcgg tctcatgctc gtgatagaaa gccgcaatgc
     3301 gcgccagtag atccttgaag cccggcagac cgtagcggga gtcccgtgga aaatacgtgc
     3361 cgccgaagaa ggggcgccgg tcatcgggtg tcaggaacat cgtcagcggc cagccgcccg
     3421 agcgctgtgt cagcatctgc tgcgcgatct ggtaaatgcg gtcgacatcg ggccgctcct
     3481 cgcgatccac cttgatgttg acgaacagct cgttcatcaa atgcgcggtg tcagggtcgt
     3541 caaacgattc gtgactcatc tggtgacacc agtggcaggc tgcatatccg atgctgacat
     3601 gcacgggctt gttctcgcgc cgcgcgcgct cgaacgcttc ctccccccat gcgtaccagt
     3661 cgaccgggtt gtgtgcgtgc tgctgcaggt aggggcttgt ctcggagatc agccgattgg
     3721 tatgggctgg tttgccttcg gggaccgggt gtgtgctgtt cacggaagtc tcggcggcgg
     3781 aagaatgttg gcgaagtcgg cctcaggtct cccgcagcat agcgcgatcc gaactgcgca
     3841 tcggtcctgc tcagctggct ccaagcgcgg ctccgcggtt ggacctgagg tttgccgtat
     3901 aatgcgcgct cccatgacgt tgaactccta catcggagcg atgtgacttc ctcctctgcc
     3961 ccagcgctcg agcatgcgtc acttccggta ctgcgcctta agcgcggcga ggagcgccgc
     4021 attgcagcgg gccacatgtg ggtcttcagc aatgaggtcg acacggcgag cacgccgctc
     4081 acggctctgg cgccgggcgc gctcgcgcaa atgcattcgc accgtgggca gtttctcgga
     4141 tacgcctatg tcaacccgca cgcgctgatc tgtgcacgga tcgtcggccg tgacatcgat
     4201 cagccgctcg atcgcgccct catcgcgcag cggctcgaga ccgcgctcgc ccttcgtcgc
     4261 aggctcaatc cggagcccta cggtcgtctc gtgttcggcg agtcggacgc gcttcccgga
     4321 ctcatcctcg atcgctacgg cgatgtggtg gtgggtcaga tcgcaaccgc cggaatggag
     4381 gcgttgaagg cggacatcgt tgccgtgacg cgtgcgctgc tgaatccctc gacgttcttc
     4441 tggaagaacg attcgggcgc tcgggatctg gagcatcttc cttcgagcgc cgaaacggcc
     4501 ttcggagaga tcccggacac gatcgacgtt ctcgaatccg gtctgtgctt tacagctccg
     4561 ctcgcccggg ggcaaaagac cggctggttc tacgatcaaa cggccaatcg tcaacgtcta
     4621 ttgcggtaca tgtggcccgg cgtgcgcgtt ctggatgtct gcagttatgt gggtgcctgg
     4681 gcggtgatgg ctttgaagca tggcgcggtg tcggcacgtt gcgtcgatgt atccgagacg
     4741 gcattgcagt acgcggcacg caatgctgcg aacaacggcg tagaggtcca gacgatccgt
     4801 gaagatgcgt tcgatgcgtt gaaggcactg caggagcagc atgcgcgctt cgatgtcgtg
     4861 attctcgatc cgccagcctt catcaagcgc aagaaggaca ttccgcaggg tcaggcggcc
     4921 tatcgcaagc tgaatcagct cgcgctagga ctgctcgaac gcgagggcct gctcgtgtcc
     4981 tgctcctgct cgtatcatct tgcgccggaa gacctggtcc aggccatccg ggccgcagcc
     5041 cgccacaccg accgcttcgt gcagattctc gaggcaggcg ggcaatcccc cgaccatcct
     5101 gtgcatccgg ccatcccgga gacccgttac ctgaaggcct tcttctgccg cgtgacgcgc
     5161 gaatagagcg ccgaacggct aagattcgcg cccgatgatc cagtatcacg cccttgaccc
     5221 catagcggtc aaaatcggcc ccctgcgggt gcactggtac ggtgtcatgt acctgctcgg
     5281 gttcggcgcc gcgtggttgc tggcccgcct gcgcgcgcgc aagccgggct cgacctggcg
     5341 gcccaccgat gtcgatgacc tgatcttctt caccatgctc ggggtcatcc tgggagggcg
     5401 catcggttac gtcctcttct acggtctggg cttctgggcg aaggatccgc tctatccgct
     5461 gcggatctgg gagggcggca tgtccttcca cggtgggatg ctgggggtca ttgcggccgt
     5521 ctccctgttt gcctggcgcc gccgccgaaa cgtcgccgat gtgctcgatt tcaccgtgcc
     5581 gctgccggga ttgggactct ttttcggccg catcggcaac ttcatcaatg gcgagctgtg
     5641 gggcaagccc accaccgtgc cctggggatt caatgtcaac ggggaagtcc gccacgcatc
     5701 gcagctctac gaagcgttct tcgagggtct cgtgctgttt gcgatcctgt ggtggttcac
     5761 ctcgcgtccg cgcccgcgtc tggcgccatc gggtttgttc ctcgtcatct acgggatcgt
     5821 gcgcttctgc atcgagttcg tccgcgtgcc ggatgagcag attggctatc tggccggggg
     5881 ctggctcacc gagggacagg tcctatccgc gcccatgatc atcggtggcc tcgcactgct
     5941 ggcgtatgcc tatcgcgtac gcaccccgtc agggaacttc tcggcagctc aatgaagcag
     6001 tacctcgaat tgatgcgcca catacgcgag agcggcctcc ggaaggcgga tcgcacgggg
     6061 accggcacat tgtcggtatt cggatatcag atgcgtttcg atctcgcccg cggattcccc
     6121 gtggtgacga cgaaacggat tcacctgcgc tcgctcatct acgagcttct gtggttcctg
     6181 cggggcgaca cgaacgtcgc ctggctgcac gagcatggcg tgacgatctg ggacgagtgg
     6241 gccaacgagg gcggcgatct gggcccggtg tacggcaagc agtggcgcgc ctggcaggct
     6301 gccgatggac gcacgatcga tcagatctca cgcctcatcg agcagctgcg cagcaatccc
     6361 aattcccgcc ggctcatcgt cagcgcctgg aacgtgggcg agctcgaaaa gatggctctg
     6421 ctcccctgcc acgcgctgtt tcagttccac gtcgccgcgg gccgcctgtc ctgccagctc
     6481 tatcagcgca gcgcggatgt cttcctcggc gtgcccttca acattgccag ctacgcgctc
     6541 ctggtgcaca tgttggcgca gcaatgtgag ctggagccgg gagagctgat ctggacgggg
     6601 ggcgattgtc acctctacct gaatcatctg gagcaggccg acctgcaact cgcgcgcgcg
     6661 ccgctggagc tcccgcgcct ggtgctgcgg cgccggccgc caagcatctt cgagtacgag
     6721 ttcgaggact tcgaaatcgt gaattaccag catcatccgg cgatcaaggc gccggtggca
     6781 atctgatcat ggcggacggg caggatgaca tcgccgggat gctcgaggtc cgccgctcgc
     6841 ccgtgcacgg gctgggtgcc ttcgcgacgc gtcggatcga gaagggagcc agggtcatcg
     6901 agtacctggg cgaacgcgtc tcgcacgagg aggcggaccg ccgttacgag tacaaagatg
     6961 ccgcggacag ccatacgttc cttttcatcg tcgacgccgg aacggtgatc gatgcgggtg
     7021 tgaacggcaa cgacgcccgc tttctcaatc attcctgcca gcccaactgc gagtccgtga
     7081 tcgagggcgg gcgcgtgttc atagaagcac tggtggaaat cgagcccggg acagagctga
     7141 catacgatta ccagattcag cgcgaggctg atgacccgga cgatgtggac gaggtattcg
     7201 cctgccactg cggattcccc gcctgtcgcg gctcgatgct gtggcccccc aggcatgagg
     7261 ccggggctac ccgctcgcgc gggcgcagga agcatacggc cagcgggacg cagccgcgca
     7321 cccgcaagga agcccgcagc ggcaaggaca cccgcagccg caccgacgcg ggcagccgca
     7381 tggacacgcg ccgcctgaag gacacgcgca ctccgaagga tgtgcgcagc ggcaaagacg
     7441 cgcgcagcca caaagacgcg cgcaggaacg gccctccgca ccgcaaatcc gctgccgcga
     7501 agcggccttc ctccaggagg gccagccggc gccgctgacg cgccggtccc acatgcaacc
     7561 acagcgtcgc tggagtctgc cggcgatcgt cggcgccacc gggatagttt acggggacat
     7621 cggcaccagt cccctgtacg ccttccagac ggcgttggat gcgatcggaa cattcgagcc
     7681 ccgagccatc ctcggtgtcc tctcgctgat cttctggagc ctgctgctgt cggttacgct
     7741 caaatacgtg accgtcatga tgcgtgccga caatgaaggc gagggcggca tcctggcgct
     7801 gttcgccctc gcgcagcgca agctggtcgt cgccagcagc tggtcgaagg cagtgatcgg
     7861 tctggcactg gcgggcaccg ctttcttctt ttgcgatgcg ctgatcactc ctgccatctc
     7921 ggtgctgagc gccgtcgagg gactcgaggt gctggatcct gacttcagcc gcggtgtggt
     7981 gcccgtcacc ctggtcgttc tggccatgct gttcctcatc cagaaacggg gctccgcgca
     8041 tgtgggccgg ttgttcggcc ccgtgatgat cgcctggttc ctcgtcattg ccatcacggg
     8101 gctggtggcc atcgtgcgtc atccgacggt gctggcagcg atggatccgc gctatggcat
     8161 ctggctgctc gtcagtcact cgacgatcgc acttgcgatc atcggtgcgg tattcctcgc
     8221 gctcaccggc ggagaagcgc tgtatgcgga catgggacat ttcggcaagg ccccggtccg
     8281 catcgcatgg ttcgcactcg tgtggccctc attggtgctg aactatttcg gtcagggcgc
     8341 gctgctgctc gagcgcggca aactcgtacc ggagagcctg tacgcgctcg ttccggcatc
     8401 gctcctgccc tggatggttc tgctcgcgac ggccgcgacc gtcattgcat cgcaggcggt
     8461 gatttccggg gcgttctccg tggcacgcca ggccgtgcag ctcgatctgt tgccccgcat
     8521 acgcgtgctg cagacttccg ccctcgagca ggcccagatc tacgtgccga tggtcaacat
     8581 gttgctgttt ctggcggtgg tggcgttcgt cgtcggattc ggctcatcga gcgcgctgtc
     8641 cggcgcctat ggtgcggcgg tcgtgggcac gatgttcatt accgacattc tcggtgcgtt
     8701 cgtcgcagtg actcagtggc aatggtcgcg ctggtcagtg gcggccgtat tcgttcccct
     8761 caccgcgatg gattccgtgt tcgtcgccgg gaacatgacc aaggtgagca ccggcggctg
     8821 gattccgctg accctcggag cgcttctgtt c
//
LOCUS       DASRHN010000384         6144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_307366, whole genome shotgun sequence.
ACCESSION   DASRHN010000384 DASRHN010000000
VERSION     DASRHN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307366"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VFB37_15550"
     CDS             complement(<1..1466)
                     /locus_tag="VFB37_15550"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZO23914.1"
                     /translation="MQQIRVRGARTHNLKNIDLDLPRDRLIVLTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMDKPDVDSIEGLSPAIAIEQKATSHNPRSTVGTVT
                     EIYDYLRLLYARAGVPRCPDHLIDLTAQTVSQMVDQVMRLAEGTPVILLAPLVSDRKG
                     EHAQILEELAAQGFVRVRIDGRIHELDALPTLDPKRKHTLEAVVDRTRVRADGAQRLA
                     ESFETALRLSGGLAKLAFLEEPGRDELVFSSQHACPVCGYSVPTLEPKLFSFNNPAGA
                     CPACDGLGTREFFDPQRVVVHPDLSLASGAVRGWDRRNAHYFQLIQSLARHYGFDIEA
                     PWTELPENVRQVLLQGSGEESIEFRYAEGKGRAMRKRHPFEGILPNLERRYRETESLT
                     VREELAKYLGVRPCPECAGTRLNRAARFVFVAERSLPEIAHLTVGQALQFFSSLSLAG
                     WRGEVAAKIVNDVSQRLRFLVDVGLDYLTLDRSAETLSG"
     gene            1596..2060
                     /gene="ssb"
                     /locus_tag="VFB37_15555"
     CDS             1596..2060
                     /gene="ssb"
                     /locus_tag="VFB37_15555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZO23915.1"
                     /translation="MARGVNKVILIGNLGADPETRAMPSGTTVANLRIATTESWRDKQ
                     SGEQQERTEWHRVALFGRLGEIAAEYLKKGSQVYIEGSLRTRKWQDKQGNERYSTEIV
                     GNEMQMLGGRGAGMGGAAPAQGSGAPGRAEPVPDYSGGPSGSREEFDDDIPF"
     gene            2362..3747
                     /gene="miaB"
                     /locus_tag="VFB37_15560"
     CDS             2362..3747
                     /gene="miaB"
                     /locus_tag="VFB37_15560"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZO23916.1"
                     /translation="MPRRYFIKTFGCQMNEYDSARMADVLQAESGLVPTEDPSQADVL
                     LMNTCSVREKAQEKVFSMLGEWRMLKARRPEVLIGVGGCVASQEGEAITERAPFVDLV
                     FGPQTLHRLPQMMAELRRTGRSVVDVSFPEIEKFDSLPAPRAEGSTAFVSVMEGCSKY
                     CSFCIVPYTRGEEISRPFDSVLAEIRTLAAQGVREVTLLGQNVNAYAGPMSDGAVVDL
                     ATLIHHIAALADIERIRFTTSHPLEFGDSLIEAFASVPKLANHVHLPVQSGSDRVLAL
                     MKRGYTGLEFKEIIRKLRRVRPDISLSSDFIVGFPGESERDFEATLALVRDVGFDQSF
                     SFLYSRRPGTPAASLPDEVPPTVKQERLGRLQAQLETQARAISERMVGTVQRVLVERA
                     ARKNAHELAGRTENNRWVNFAGPATLIDRFVSLTVTETRAHSLRGRLLSAPEVDRGAR
                     SFSMSAASTAS"
     gene            3756..4715
                     /locus_tag="VFB37_15565"
     CDS             3756..4715
                     /locus_tag="VFB37_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZO23917.1"
                     /translation="MASPASEPQRDFDLESDNARLANLCGPLDENLRLLETRLDVEIR
                     RRGDNFRVIGAQAASAEQTLRELFDLAKKEEVTPEHVHLALRERENGHLQPESDQQEH
                     AVRVPRGGIRARGAHQREYLAHIRTGDLTFGIGPAGTGKTYLAVACAVEALQADRIRR
                     VVLVRPAVEAGERLGFLPGDLTQKVDPYLRPMYDALYEMMGFDRVARFIERNVIEVAP
                     LAFMRGRSLNESFIILDEAQNTTIEQMKMFLTRIGFGSRAVVTGDITQTDLPPGKQSG
                     LAHVIDVLKDVEGVAFTFFDAQDVVRHPLVQRIVQAYEAWRRS"
     gene            4700..5164
                     /gene="ybeY"
                     /locus_tag="VFB37_15570"
     CDS             4700..5164
                     /gene="ybeY"
                     /locus_tag="VFB37_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZO23918.1"
                     /translation="MAPLVRLSVDLQLRVRTWAPPRTHIAAWASMALGRQGAGRELGV
                     RVVGPAESRRLNARYRGRNRPTNVLSFPAPRLPEGPQQPLGDLVICPAVLRAEAHAQR
                     KSLRAHWAHLVVHGTLHLIGYDHERPAQASRMERREVALLRRLGFPDPYRSA"
     gene            5164..6039
                     /locus_tag="VFB37_15575"
     CDS             5164..6039
                     /locus_tag="VFB37_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZO23919.1"
                     /translation="MAKDLNTTGRWLKRITQTFSGEPQDRDELLTILRDAAERGLVDS
                     DARTMLEGVLEVSDLQVRDIMVPRAQMVCVRRDDLAAAILPVVVESGHSRFPVMDADR
                     DDIVGILLAKDLLRLCISARQEPFDIREYMRPAVFVPESKRLNVLLKDFRGNRNHMAI
                     VVDEYGGVSGLVTIEDVIEQIVGEIDDEFDVEDDQNIRKEADRQYTIRGVTRIEDFNE
                     YFGSRLSEEEGFDTIAGLLMKQLGRLPRRGEVASIDGFEFRVARADRRRIDTLRVVSP
                     RDVVPPAERQTAGES"
BASE COUNT         1102 a         1971 c         2023 g         1048 t
ORIGIN      
        1 ccggacagcg tctccgcgct gcgatcgagc gtcagataat cgagccccac atcgaccaga
       61 aagcgcaatc gctgcgacac atcgttgacg atcttagcgg ccacctcgcc ccgccagccc
      121 gctagactca aggaactgaa gaactgcagt gcctggccga ccgtgaggtg cgcgatctcg
      181 ggcagactgc gctcggccac aaagacgaag cgagctgcac ggttgagacg tgtgccagcg
      241 cattcgggac acggacgcac gccgaggtat ttcgccagct cctcgcgcac cgtgagggac
      301 tcggtctcgc ggtagcggcg ctcgaggttg ggcagaatgc cttcgaacgg atggcgtttg
      361 cgcatcgcgc gccccttgcc ctctgcgtag cggaactcga tgctttcttc accactgccc
      421 tggagcaata cctggcgtac gttctcgggc aactccgtcc acggcgcctc gatatcgaat
      481 ccgtaatggc gggcgagaga ctggatgagt tgaaaataat gagcgttgcg ccggtcccaa
      541 ccgcgcaccg ctccgctcgc cagcgacaga tccggatgca cgaccacgcg ctgcggatca
      601 aagaattctc gggtccccag accatcgcag gccgggcagg ctcccgcggg attgttgaat
      661 gagaacagct tcggctcgag cgtcggcacg ctgtacccgc acaccgggca ggcgtgctgg
      721 ctggagaaca ctagctcgtc gcggccgggc tcttcgagaa atgcgagttt cgcgagcccg
      781 cctgaaaggc gcagcgcggt ctcgaaggac tctgcaagcc gctgtgcccc gtcggcgcgt
      841 acgcgggtac ggtctacgac cgcctcgagc gtgtgcttgc gtttcggatc gagagtgggc
      901 aacgcgtcga gctcgtggat tcgtccatcg atgcgaaccc gcacgaagcc ttgtgcggcg
      961 agctcttcca ggatctgcgc atgctcgccc ttacggtcgc tcaccagcgg agcaagcaga
     1021 atcaccgggg ttccttccgc cagcctcatc acctggtcca ccatctggct tacggtctgc
     1081 gccgtcaggt cgatgaggtg atccggacag cgtggcactc cagcgcgcgc gtagagcaac
     1141 cgcaggtagt cgtagatctc ggtcacggta ccgaccgtcg agcgaggatt gtgcgaggtg
     1201 gccttctgct cgattgcaat ggcgggcgac agcccctcga tgctgtccac gtcaggcttg
     1261 tccatcatgg agaggaattg ccgggcatag gcggacagcg actcgacgta gcgacgctga
     1321 ccttcggcat ataaggtgtc gaacgcgagt gatgacttac cggagcccga cagtccggtg
     1381 agcacgatca gccggtcccg cggcaaatcg agatcgatgt tcttcagatt gtgagtgcgc
     1441 gccccgcgga cgcggatctg ctgcatgcaa agcttccggc aaacatatga gtgtacccgc
     1501 cgcgggcgca aaattcggca atcacagccc ctcacgcact acgcggcggc cccgcggtcc
     1561 actagaatgt tcggatcgca atcgagggcc cagccatggc gcgcggagtc aataaagtca
     1621 tcctgatcgg aaacctcggc gccgaccctg agactcgcgc catgccctcc ggaaccaccg
     1681 tggccaatct gcggattgcg accaccgaga gctggcgtga caagcagagc ggtgagcagc
     1741 aggagcgcac cgaatggcat cgcgtggccc tttttggacg cctcggagag atcgctgcgg
     1801 aatatctgaa gaaaggctcg caggtctata ttgagggcag ccttcgcacc cgcaagtggc
     1861 aggacaagca gggcaacgag cgctactcga ccgagatcgt cggtaacgag atgcagatgc
     1921 tgggtggccg cggcgccggc atgggcggcg ctgcacccgc ccaggggtcc ggggccccag
     1981 gccgtgccga gccggtcccg gactactctg gcggtccttc cggcagccgg gaagagttcg
     2041 acgacgacat acccttttag tctgagggct gaaggacgcc tgcggcgctt tcagcccgcg
     2101 cctgacgtcc tgaaagacgc ctgcggcgcg ttcaggccgc gcccgatgtc ctttgcgatc
     2161 cttggcgccg ccgcaaaagg cgtggctttt gcctcccgtg gcccctcagc gccgcagcaa
     2221 cggggcccga acgacgcacc gcggggcccg gaaacgggca taagaaggcc tagcgggggg
     2281 ggttccgccg gccggggagg gccgggcgcc ttagccgtgc gcccctgccg ccaagggggg
     2341 tagaattctt cggcaccgcg tatgccccgc cgctatttca tcaagacttt tggctgccag
     2401 atgaatgagt acgattccgc ccggatggcg gacgtgctgc aggccgagag cggactggtg
     2461 cccaccgagg atccttccca agccgatgtg ctgctcatga acacgtgctc cgtgcgcgag
     2521 aaggcgcagg agaaggtgtt ctccatgctg ggtgaatggc gcatgctcaa ggcgcgtcgc
     2581 cccgaggtcc tcatcggtgt gggcggctgc gtagccagtc aggaagggga ggcgataacg
     2641 gagcgcgcgc ccttcgtgga cctcgttttc ggcccgcaga ccctgcaccg cctgcctcag
     2701 atgatggccg agctgcgccg caccggccga tcggtggtcg acgtgagctt cccggagatc
     2761 gagaagtttg acagcctgcc ggccccccgg gcggaaggct cgactgcgtt cgtctcggtg
     2821 atggagggct gcagcaagta ctgcagcttc tgcatcgtcc cctacacccg cggcgaggag
     2881 atcagccggc cgtttgactc cgtcctcgcc gagatccgca ctctggctgc ccagggcgtt
     2941 cgtgaggtca cgttgctcgg acagaatgtc aacgcgtacg ccggcccgat gtcggatggc
     3001 gcggtcgttg acctggcaac actgattcat catatcgccg ccctggcgga catcgagcgg
     3061 atccgcttca cgacctcgca cccactcgaa ttcggcgaca gcctgatcga ggcgttcgcg
     3121 agcgtgccca agctggcgaa ccacgtgcac ctgcccgtgc agagcggctc cgatcgggtg
     3181 ctcgcgctga tgaagcgcgg ctacacgggg cttgaattca aggaaatcat ccgcaagcta
     3241 cggcgggtgc ggccggatat cagtctctca tccgacttca tcgtaggttt cccgggcgag
     3301 agcgagcgtg acttcgaagc cacgctcgcg ctcgtgcgcg acgtggggtt cgatcaatcc
     3361 ttcagcttcc tctacagcag gcgtcccggc actcccgcgg cgtccttgcc tgacgaggta
     3421 ccccccacgg tcaagcagga aaggcttggc cggctgcagg ctcagctcga aacgcaggcc
     3481 cgcgcgatca gtgaaaggat ggtcggcacg gtgcaacgcg ttctcgtgga gcgtgccgcc
     3541 cggaaaaatg cgcacgagct cgccgggcga accgagaata accggtgggt caacttcgca
     3601 ggacctgcca cgctgatcga tcgcttcgtg agcttgacgg tgaccgagac gcgagcgcac
     3661 tccctgcggg gccggctgct gtcggcgccc gaggttgatc gcggcgcccg atccttttcc
     3721 atgtccgccg cgagcacagc ctcctgaaag caccagtggc ttcaccagcg tccgagccgc
     3781 agcgcgactt cgatctcgag tcggacaatg cgcgattggc caatctctgc ggcccgctcg
     3841 atgaaaacct gcgcctgctc gaaacacgcc tggatgtcga gattcgccgt cgcggcgaca
     3901 acttccgtgt gatcggcgcg caggctgcca gcgccgagca gaccttgcgc gagctgttcg
     3961 atctggcgaa gaaggaggaa gtgacaccgg agcacgtgca tctcgcgctg cgagagcgtg
     4021 agaacggaca tctgcaaccg gaatcggatc agcaggagca tgccgtgcgt gtcccgcggg
     4081 gcgggatccg ggcgcgcggc gcgcatcaac gggagtatct tgcacacatc cgcaccgggg
     4141 acctgacatt cgggatcggg cccgccggca ccggcaagac ttacctcgca gtcgcctgtg
     4201 cggtcgaagc gctgcaggct gacaggatcc gcagagtcgt gctggtcagg ccggcggtcg
     4261 aagccggcga gcggctcggg ttcctccccg gagatctcac gcagaaggtc gatccgtatc
     4321 tgcggccgat gtacgacgcc ctgtatgaaa tgatgggatt cgaccgtgtg gcgcgcttca
     4381 tcgagcgcaa cgtgatcgag gtggcgccgc tcgccttcat gcgcggccgc tcgctcaacg
     4441 aatccttcat cattctcgac gaggcccaga acaccacgat cgagcagatg aagatgtttc
     4501 tgacccggat cggcttcggc tcccgcgcgg tcgtcaccgg cgatatcacg cagacggacc
     4561 ttccgcctgg caagcaatcc ggcctcgcgc atgtgatcga cgtgctcaag gatgtcgaag
     4621 gggtggcatt caccttcttc gatgctcagg atgtcgtacg gcatccgctc gtgcagcgga
     4681 tcgtgcaggc ctatgaagca tggcgccgct cgtgaggctg agcgtggacc tgcagcttcg
     4741 ggtgcgcacc tgggctccgc cacgaactca tattgcagcg tgggccagca tggcgctcgg
     4801 acgccagggc gccggccgcg agctgggcgt gcgcgtggtc ggtccagcgg agagtcgtcg
     4861 cttgaacgcg cgttatcgcg gacgcaaccg tccgaccaac gtcctgtcat ttcccgcgcc
     4921 gcgattgccc gagggcccgc aacagccact cggagatctg gtcatctgtc cggctgtgct
     4981 gcgcgccgag gcgcacgcac agcgcaagag cctgcgagca cactgggcgc acctggtggt
     5041 gcatggcacg ctgcatctga tcggttacga tcacgagcgc cccgcacagg ccagccgcat
     5101 ggagcgccgc gaggtggctt tgctgcgtcg gctcggcttt cctgacccgt accggagtgc
     5161 ctgatggcca aggacctcaa taccaccgga cgttggctga agcgaattac gcaaaccttc
     5221 tccggcgagc cgcaggaccg cgacgagctg ctcaccatac tgcgcgacgc agccgagcgc
     5281 ggtctggtgg attccgacgc ccgaaccatg ctcgagggcg tgctggaggt gtccgacctg
     5341 caggtgcggg acatcatggt gccgcgcgcc caaatggtct gcgtccgccg cgacgatctc
     5401 gccgcggcca tcctgccggt ggtcgtcgaa tcgggccact cgcgcttccc tgtgatggat
     5461 gccgatcgcg atgacatcgt gggcatcctg ctcgccaagg atctgctgcg actgtgcatc
     5521 tcggcccggc aggagccgtt cgatatccgc gagtacatgc ggcccgcggt gttcgtcccc
     5581 gaatcgaagc ggctcaacgt gctgctgaag gactttcgcg gcaaccgtaa ccacatggcc
     5641 atcgtcgtgg acgaatatgg cggggtatca ggactggtca ccatcgagga cgtgatcgag
     5701 cagatcgtgg gcgagatcga cgacgaattc gacgtggagg acgatcagaa catccgcaag
     5761 gaggctgacc ggcagtacac gatccgcggg gtcaccagga tcgaggattt caacgaatac
     5821 ttcggctcga ggctgtccga ggaggagggc ttcgatacca ttgccgggct gctgatgaag
     5881 cagctgggcc gtctgccgcg acgcggcgag gtcgccagca tcgacgggtt cgagttccgg
     5941 gtggcgcgtg ccgaccggcg gcgcatcgat accctgcgcg tcgtttcccc gcgcgacgtc
     6001 gtcccaccgg ctgagcggca gaccgccggc gagtcctgat ccgatgaatg tacggcgcga
     6061 agccgccgtc acgaaccccg tgccgggcag cgggtcgggc gtgacagcgg aagagactgg
     6121 gcgtgtcctg gagcgcttgc tgcc
//
LOCUS       DASRHN010000385         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_140813, whole genome shotgun sequence.
ACCESSION   DASRHN010000385 DASRHN010000000
VERSION     DASRHN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140813"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFB37_15580"
     CDS             complement(<1..234)
                     /locus_tag="VFB37_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23920.1"
                     /translation="MPAKKPAPKAAKGKKAASRTPARKATVTRKPADRVKVVAVSHTR
                     KSPVAPKKPAPHRSTQTTEEPIEMRKAAEPAPEP"
     gene            complement(437..826)
                     /gene="queF"
                     /locus_tag="VFB37_15585"
     CDS             complement(437..826)
                     /gene="queF"
                     /locus_tag="VFB37_15585"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZO23921.1"
                     /translation="MPSRPAFELESFANPCINRDYTIRMRIPEFTCLCPKTGQPDFAT
                     LCLEYVPDRLCVELKSLKLYIWSFRDRGAFHEAITNEIADQLAGTLAPRFLRLTARFN
                     VRGGIYTSVIAERRQDGWQAPESVTLP"
     gene            877..>3494
                     /gene="smc"
                     /locus_tag="VFB37_15590"
     CDS             877..>3494
                     /gene="smc"
                     /locus_tag="VFB37_15590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZO23922.1"
                     /translation="MRLTKIKLAGFKSFVDPTQVSFPSNLTGVVGPNGCGKSNVIDAV
                     RWVMGELSAKHLRGDNMTDVVFNGSAARKPVGTASVELVFDNADGKITGPYASYNEVS
                     LKRHVARDGTSAYYINAARCRRKDITNLFLGTGLGSRSYAIIEQGMISRVIEAKTEDM
                     RAFVEEAAGISRYKERRRETESRIGETRENLERLQDVRDEVDKQIRHLQRQAATARRY
                     QAFKDQERRLTAELLALKLRDLDSGAEVHDSAMRERELAMQAALADQRAAEAAIEKQR
                     AFHTEQSEQLSRVQARYYEVGAEISRLEQAIQHARELRERQRADLAQTHATLADLGSH
                     VERDEQQLSELREEIGQLAPQLQAVQRAESVAAAGLESAEQELQDWQQRWEELNRALG
                     AAHQTTQVERARIEQLENQMRRHVAQADRLALERETLTAQDASAQLAALADQEAAARG
                     ARDELSGKLASALEQAQALRASQLAVEAELEGARGTRERARAEQTALEALQRAALTHD
                     AGSESEWLAGAGLSARPRVTETLEVQSGWERAVETALGEYLEAICVDGLDEHEEALSG
                     LATGRIGLVETGGAPRRGGGETLAAKVSGPAAVVLQLVNILTAESLAEALRARASLSA
                     GQSIITRGGEWVGRGWLRVSRNADRHAGVIEREHRLKGLRSAVAEAEAAVSDAESRLA
                     AVRESLSKAEAQRDEAQSSIQSAHRAHAELLSQLEATRARVEESGLRRERLNTESAVV
                     AQEDGVTREALARARAELERGLSLLNELDSRRHELESEREERRAAVVSARGAAQSTQV
                     ASRDLLIRIESRRSTENSVTIGLSRMLEQRLQLSQRRDELAAELAGGDEPIVHFEQRL
                     DDSLARRLQVEADLSAA"
BASE COUNT          609 a         1092 c         1260 g          533 t
ORIGIN      
        1 cggctcgggc gccggctctg cagcctttct catctcgatg ggttcttccg tcgtctgggt
       61 ggaccgatgg ggtgcaggct ttttcggcgc aaccgggctc ttgcgggtgt gggagaccgc
      121 taccaccttt acacgatcgg cgggcttgcg ggtcaccgtg gcctttctgg ccggtgtccg
      181 gcttgccgcc ttcttaccct tcgcggcttt gggcgcgggt ttcttggccg gcatcaatcg
      241 ctcctcgaag cagcagccct gtacccccca cggaccaaac cgaggctcga tccgtggatc
      301 ttcaccgttg accacaggac atcggtttct caggcccggt gaaggacgcg ggattatacc
      361 ggccgaggcc gacttgtcac ggccgaccgc gattattggt cgcggcagcc tcgagggctc
      421 ctcgggaggg cgccgctcag ggaagcgtga ccgactcggg ggcctgccat ccgtcctggc
      481 ggcgctccgc gatcacgctg gtgtagatgc cgccgcggac gttgaagcgg gctgtcagcc
      541 gcaggaaccg tggcgccagc gtgccggcca gctggtcggc gatctcgttg gtgatggcct
      601 catgaaatgc gccgcgatcg cggaaggacc agatgtagag cttcagcgac ttcagctcca
      661 cgcacagcct gtcaggcacg tactccaggc agagcgtggc gaaatccggc tgccccgtct
      721 tcggacacag acaggtgaat tccgggattc gcatgcggat cgtgtagtcg cgattgatgc
      781 aggggtttgc gaaactctcc agctcgaagg caggtcgtga cggcattctt ccaattcctc
      841 tacactacgc ggcgcacgtt ccatacgaga tacccgatgc ggctgaccaa gatcaagctc
      901 gccggtttca aatcgttcgt cgatcccacc caagtcagct ttcccagcaa tctcaccgga
      961 gtcgtcggtc cgaacggctg cggcaagtcc aacgtcatcg atgcggtgcg ctgggtgatg
     1021 ggagagctgt cggcgaagca tttgcgtggc gacaacatga cggatgtcgt cttcaacggc
     1081 tcggcggccc ggaaaccggt cggaaccgcc tcggtcgagc tggtgttcga caacgccgac
     1141 ggcaaaatta ctggccccta tgccagctac aacgaggtct cgctgaagcg gcacgtggcg
     1201 cgcgacggga cctcggccta ttacatcaat gccgcccgct gccgccgcaa ggacatcacg
     1261 aacctgttcc tcggaaccgg cctcggctcg cgcagctacg cgatcatcga gcagggaatg
     1321 atctcgcggg tgatcgaggc gaagaccgag gacatgcgcg ccttcgtgga ggaggccgcg
     1381 ggaatatcgc ggtacaagga gcggcggcgc gaaaccgaaa gccgcatcgg ggagactcgc
     1441 gaaaacctcg agcggctgca ggacgtccgc gatgaggtgg acaagcagat ccggcatctg
     1501 cagcgtcagg cggcgaccgc gcgccgttat caggccttca aggaccagga gcgcaggctg
     1561 accgcggagc tgctggcgct gaagctgcgc gatctggaca gcggagcgga agttcacgac
     1621 agcgccatgc gcgagcgcga gctggcgatg caggccgccc tcgcagacca gcgggcggcc
     1681 gaggcagcca tcgagaagca gcgcgccttc cacaccgagc agagcgaaca gctcagccgc
     1741 gtccaggctc gctactacga ggtcggcgcg gagatctccc gcctggagca ggccattcag
     1801 cacgcccgcg agctgcgcga gaggcaacgt gcggacctcg ctcagacgca tgccaccctt
     1861 gccgatctgg gatcgcacgt cgagcgtgac gagcagcagt tgagcgagct gcgcgaagag
     1921 atcgggcaac tggcgcctca gctgcaggcg gttcagcggg cggagagcgt ggccgcggcg
     1981 ggactcgaat ccgctgagca ggaattgcag gactggcagc aacgctggga ggagctgaac
     2041 cgggcgctgg gcgctgcgca tcagaccact caggtcgagc gtgcccgcat cgagcagctg
     2101 gagaaccaga tgcgccggca tgttgcccag gccgacagac tcgcgctcga gcgcgagacc
     2161 ctgacggccc aggacgcgag cgcgcagctc gcggccctcg cggaccagga agcggcggcc
     2221 cgtggtgctc gggatgagct gtcgggcaag ttggcctcgg cgctcgagca agcccaggcg
     2281 ctgcgcgcga gtcagcttgc ggtcgaggcc gagctggaag gagcgcgggg cacgcgcgag
     2341 cgggcccggg ccgagcagac cgcgctcgag gccctgcagc gcgccgcgct gacgcacgat
     2401 gcgggctcgg agagtgagtg gttggccggg gcgggactgt ccgcgcgccc acgcgtgacg
     2461 gaaacgctcg aggtgcaaag tggctgggag cgagcggtgg agaccgcgct cggcgaatat
     2521 ctcgaggcga tctgcgtcga tgggctggac gagcatgagg aggctctgtc aggcctggct
     2581 actgggcgca tcgggctggt cgagacaggc ggcgcaccgc gccggggagg gggcgagacg
     2641 ctcgccgcca aggtgagcgg ccctgcggca gtcgtgctgc aactcgtcaa catcctcact
     2701 gcggagagtc tggctgaagc cctgcgggcg cgagcgagcc tctcggcggg ccaatccatc
     2761 atcactcgtg gcggcgaatg ggtcggtcgt ggttggctgc gtgtcagtcg caatgcagat
     2821 cgtcacgcgg gcgtcatcga gagggaacat cgactcaagg gactgaggag cgccgtggcc
     2881 gaggcggagg ccgctgtcag cgacgcggag agtcgcctcg cagcggtccg cgaatccctg
     2941 tcgaaggccg aggcgcagcg ggatgaggcg cagagcagta tccagagtgc ccaccgcgcg
     3001 cacgccgagc tgctgagcca actcgaggcg acccgtgcgc gggtcgagga gtcgggcttg
     3061 cgccgcgagc ggctgaacac cgagtccgcc gtggtcgctc aggaggatgg tgtgacgcgc
     3121 gaggcgctcg cgcgtgcacg cgccgagctc gagcgcggat tgtccctgtt gaatgagctg
     3181 gactcgcgcc gccacgagct cgagagcgag cgcgaggaaa ggcgcgccgc ggtggtgagc
     3241 gcacgaggcg cggcgcagag cacgcaggtg gcatcgcgag acctgttgat ccgcatcgag
     3301 tcgcggcgct ccacggagaa ctcggtgacc atcggcttga gccgcatgct cgagcagcgc
     3361 ctgcagcttt cacagcgccg ggacgagctg gcagcggagc tcgctggcgg ggatgagccg
     3421 atcgtgcatt tcgagcagcg tctggatgac tcgctcgccc gtcggctgca ggtcgaggcg
     3481 gatctgtctg cggc
//
LOCUS       DASRHN010000386        21258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1620367, whole genome shotgun sequence.
ACCESSION   DASRHN010000386 DASRHN010000000
VERSION     DASRHN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21258
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620367"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(650..832)
                     /locus_tag="VFB37_15595"
     CDS             complement(650..832)
                     /locus_tag="VFB37_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23923.1"
                     /translation="MFKQVVPVLSLILLAGPVFAADTPPPADQSASAPAKSVKKHHGK
                     KHHKASTSTDQSAQPK"
     gene            complement(977..1522)
                     /locus_tag="VFB37_15600"
     CDS             complement(977..1522)
                     /locus_tag="VFB37_15600"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZO23924.1"
                     /translation="MDPGTVQEIKDAIRTIPDYPKPGIQFRDITTLLGNARTFRRVVD
                     ELVEPWTTAKVNRVAGIEARGFILGGAVAHQLAAGFVPIRKKGKLPHTTVRVAYSLEY
                     GLDEMEMHQDAVLPGERIILVDDLIATGGTAEGAVRLLRALGAQILAGCFIIDLPELG
                     GRRKLEALGVPVRTLVAFEGH"
     gene            complement(1527..2180)
                     /locus_tag="VFB37_15605"
     CDS             complement(1527..2180)
                     /locus_tag="VFB37_15605"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HZO23925.1"
                     /translation="MTIEQQTLSEACERNKHPILRVLTASFASTRSVLEVGSGTGQHA
                     LHFSAHLPHLTWQPSEVGENLAGLRARIAQQGPANLREPIELDVRARPWRVAPVDGLF
                     SANTLHIMSWSSVQEFFRGLDTALVEPAVVCIYGPFRYAGRHTSTSNAAFDDFLRARD
                     PHSGIRDFEAVQRLASAAGLVLLADHAMPANNRTLVWGKPAGARQGGWSQGQETGVE"
     gene            2305..2871
                     /locus_tag="VFB37_15610"
     CDS             2305..2871
                     /locus_tag="VFB37_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZO23926.1"
                     /translation="MQAIEALLQRHSARALGEPAPDDAALALMFQSAAHAPDHGRLRP
                     WRFILIRHDARGQLGELLAEHLRRTRQPISEEALERERQKAFRAPVIIAVAAIIALNA
                     KVPPIEQVLSAGAAAYGLMLAAFSLGFNAMWKTGGPAYDETVKSALGLAQKDALVGFL
                     YVGTEERSAQPPARPAWQQFVQEWQRPS"
     gene            complement(2872..4998)
                     /locus_tag="VFB37_15615"
     CDS             complement(2872..4998)
                     /locus_tag="VFB37_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO23927.1"
                     /translation="MNTALSLKPPSSPAEADSAPDNARMLKTLLANVDGMVYRRRLDA
                     DWTMEFVSNGCLRVIGRQAAELLSEPLLSYSELLLPEDRMWVREAIRCAVEEGRAFDL
                     EYRIVHADGTTRWVWERGTGVADEHGKTVALEGLIQDITKRKHAEHALREAERRYHGL
                     FDNAIEGIFRTTTDGQFLDANPALAHIYGFESPEELMAWVRDISSQLYVEKNRRDEFM
                     HIVKARGSVTGFESQVYRKNGDIIWISENARAVHGEDGGLIGYEGTVEDITERRLYQV
                     RIEHQANYDTLTGLANRSLLQDRLQQALLTAANSDRRIAVAFVDVDRFKFINDSLGHH
                     LGDELLKTVAARLRSCVRDCDTVARLGGDEFVLLINGHGGPDSVRQLMAHMLSVLSQP
                     WLIEQKEFLVSCSIGVALHPEDGEEALTLLKHADSAMYRAKDSGRNNFQFFTRELNAL
                     TTERLELEMHLRRALERNQFVLRYQPRVNLATGQIVGAEALLRWRIPQRGTIAPRRFI
                     SLAEETGLIVPIGKWVLQTACAQNKAWQDAGLPPIVVSVNVSPRQFRQESLVQTVAEA
                     LQNSGLDPHFLELELTESMVMHDAPQLVAMLDELKRLGVQMAVDDFGTGYSSLSYLKR
                     FPVDRLKVDRSFVEHMSTEPDDATIVRAIIALGHNLGLKVVAEGVESAQQHRALRAYQ
                     CDEAQGFLFSRAVSARDLPRLLTRSK"
     gene            complement(5129..6841)
                     /locus_tag="VFB37_15620"
     CDS             complement(5129..6841)
                     /locus_tag="VFB37_15620"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO23928.1"
                     /translation="MKVRFRLSALFPASWHNSSTFAPFQSPVFAAIWTASLISNFGSL
                     IQAVGAAWMMTSIAPSADMVALVQASTTLPIMLLSLPSGATADVWDRRLVMIIAQALM
                     LLVAGALSVIGYLGLITPWLLLALTFLIGCGTALYGPAWQSSVGEQVSRADLPSAVAL
                     NSLAFNLARAAGPAIGGVIVAQAGPPAAFLANALSYVGLIVVLILWRRPRPAALLPPE
                     NVVMAIGAGVRYARLSPEIRTVLVRGIVFGLLGSTVWALMPLIARDLIGGNAVTYGVL
                     LGAFGTGAVLGALGLAALRERLTNERIVQISTAGFGASSIVTALSAWHALSMVALMIG
                     GACWVLALSTFNVTVQTSSPRWVVGRTVAIYQMVTFGGLAIGSWLSGEVASEFGLARC
                     LMLSGVLMVVSTLLGRWLPLRQPEGLNLDPSRTWSSVNRAQLERLMDSGPVVVTVEYR
                     IAPPDTEGFRLAMKELRRIRRRDGAKRWSLMQDLAVPEIWLERYHSPNWIEHVRRHHR
                     FTVSDQEIEQRVLAFHQGEQPPRIRHLIERRLGDGMAERTAATDPNLPSGPVSLASSG
                     SEES"
     gene            complement(6948..7364)
                     /gene="hemJ"
                     /locus_tag="VFB37_15625"
     CDS             complement(6948..7364)
                     /gene="hemJ"
                     /locus_tag="VFB37_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HZO23929.1"
                     /translation="MLWLKAFHLVFVVTWFAGLFYLPRLFVYHAAASDSLGLERFAVM
                     ERRLFGIMTIGAALAILFGVLMILASPAYLQAGWLRLKLVLVLALIGYHCWCGALMSS
                     LRSGRNTHSQRWYRLFNEVPALLLIAIVVLAVVKPW"
     gene            7520..9196
                     /locus_tag="VFB37_15630"
     CDS             7520..9196
                     /locus_tag="VFB37_15630"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006586706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZO23930.1"
                     /translation="MTLRNIEIAQRAKMKPIAQIAQERLGLPEDSLSLYGRYKAKVSL
                     DYVRQLDARPEGKLILVTAISPTPAGEGKTTTTVGLGDALNFTGRKTLICLREPALGP
                     VFGMKGGAAGGGYSQVVPMEDINLHFTGDFAAIALANNLLAALIDNHIHHGNPLGFDL
                     RRIGIRRVVDVNDRALRETVAGLGGPANGYPRQDGFDIVVASEVMAIFCLATSLADLR
                     ERLGKIVVGYTSAQKPILAKDLKAHGAMTVLLKDALAPNLVQTLENNPAFIHGGPFAN
                     IAHGCNSVVATRTALRLADYVVTEAGFGADLGAEKFVDIKCRKANLRPSAAVIVATVR
                     ALKYHGGVEVAELGKENLGALEDGISNLRRHVENVRNNYGLACVVAINHRAEDTSREV
                     QRLVDLVEPSGVKVVLGRHFAEGSQGAVDIANEVVRLCEQPSSFKFVYDEHATLWEKI
                     RAIATKVYHASDVDADAKVRAQIDRLQHSGYGHYPVCVAKTQYSFSTDPKLRGAPTHH
                     VVIIREVRLAAGAEFIVMICGDIMTMPGLPAVPAANSIDIDENGKVVGLF"
     gene            complement(9286..9945)
                     /locus_tag="VFB37_15635"
     CDS             complement(9286..9945)
                     /locus_tag="VFB37_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23931.1"
                     /translation="MIRVVLLVLLAFGPGLLAGCAGGDETSKVLGSVRIAAGERTGDA
                     TTVNGSIDLGANCVVKDAETVNGSVKLHEHAFAQSVKTVNGSVHLNQGARVAGSVDVV
                     NGSVLLDRGADVGGRVTNVNGSIHLESAHVGGGIETSSGDIALGGASHIEGGIRVTND
                     NAGWFHFGSWRRPRIVIGPDAVVQGSLIFKREVQLYVSDRAQIGPVEGATATRFSGAE
                     P"
     gene            10042..10794
                     /locus_tag="VFB37_15640"
     CDS             10042..10794
                     /locus_tag="VFB37_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO23932.1"
                     /translation="MKKASRAKGRRPRRILVLDVGGSHVKFRVGPRGKTGRFVSGPHM
                     TPQKMTRTLLELQPRSAYDAVSIGYPGLVLHGRITAEPHNLGHGWVHFDFEKILGAPV
                     RVINDAAMQAIGSYAGGRMLFLGLGTGMGATLILEGVVEPLEIGHLPYRDGHSFEDYV
                     GEHGRSQLGNKKWRKVVREVVAQLSKALETDYVVIGGGNAKRLLSLPTNCRLGDNRNA
                     FAGGLRMWQHSSDPLQISSQRTRRRKSPVNAV"
     gene            10856..11443
                     /locus_tag="VFB37_15645"
     CDS             10856..11443
                     /locus_tag="VFB37_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23933.1"
                     /translation="MKAIATCLGALAAGLLCSAAMAQNAQQDQQRDVNQQDRIEQGLK
                     SGELSTREAGQLERQQQHIDGMEAKDLKNGSISAGEQARLNSAENRASRDIAADKHNA
                     VTGNAGSASSERMQADVQRNVNQQQRIANGMNSGALTNREAGSLERGQAHVDRREANA
                     AANGRVGAGEQRRIQRSQNRQSGRIYRKKHNLRTK"
     gene            11561..12337
                     /locus_tag="VFB37_15650"
     CDS             11561..12337
                     /locus_tag="VFB37_15650"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HZO23934.1"
                     /translation="MPFTLAHPAAVLPLRRFRFLLTVPLVIGSVVPDVPYYFPRRIAQ
                     LWNDTHTLDGSFFICLPLGMALLVAVVLLRDPLTVLLSPRARTACLRSIEEFAKRPMH
                     WPIALLSLLIGTWTHIGWDSFTHPDGWMTMRVAALGAPISLFGWDTTMSHLLQYVSSI
                     VGLVAVAVWIRRAVRRTPAPANAEILPSRLRRTLLLMVTASTLLIVSMLAWRQEWYRD
                     SYYHLSYLLLTRTIAWFGTLYLGAGLAVMFNRRAVPEPVA"
     gene            complement(12345..12872)
                     /locus_tag="VFB37_15655"
     CDS             complement(12345..12872)
                     /locus_tag="VFB37_15655"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HZO23935.1"
                     /translation="MAEVEIHTGHDHAGDTFAQRVGVAVGVIGILLAVVTIASHRAHT
                     QAVINRTEANDQWSFYQAKKVRQQVLEVGAGLARTLAADESKVQGLIERYSAQGARYA
                     QETADIEKVARAAEEETRREERRALRLDLAEGFLELGLVLSSLYFLARRRFFPALGLS
                     AAVIGTALGIAGLLV"
     gene            complement(12927..13718)
                     /locus_tag="VFB37_15660"
     CDS             complement(12927..13718)
                     /locus_tag="VFB37_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZO23936.1"
                     /translation="MLPLQAFGDLAGAVNLVRLHGCPGSVGGAPRLRENPRLDQAARL
                     LARGENGHAAMERAGYRAVSSAFVRMTHVSDDRAVEALVSRRFCSQALDKAMREIGTH
                     RQGPDVWLVLAAPFLPPRPEAQAVVARRVLELTNLARSKPHRCGNVLYPAASPLVLAP
                     ALEAAALEHSRDMAAHGYLDHTGHDGSSPPDRVSRTGYRWRVVGENLASGVMTADEAV
                     SGWLASPHHCENVMTAGFSQMAVAYAVNASSPGGIYWTQVFATPR"
     gene            13768..14973
                     /locus_tag="VFB37_15665"
     CDS             13768..14973
                     /locus_tag="VFB37_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZO23937.1"
                     /translation="MDEGTRLGHLWSMPVSRESNPETPTASWPWREGNRFLLLEASEQ
                     YLERMVAAIDDARSYVLLEMYLVESGVIAGRFVEALVRSARRGVRVHVIVDGFGSLAL
                     SQADRRELLDSGVELRVYNPLRLAKRLRNFIRDHRKLMLIDGEVAFVGGIGLTDAFGV
                     VGPPGWPWRDLVAEIHGPVVADWQRAFAQTWRTSGSPLLIPVPTIGIVSGEGARGRVA
                     LSEAWYRSELASAVARRIIEARGRAWIMSAYFVPSRRFRKALRRAARRGVDVRLLVPG
                     PLTDHPWVRQAARRFYGKLLRNGVRIFEFQPRVLHGKMTICDDWVSVGSSNLDRWSFK
                     WNLEANQEVESAALAQAAAAVFEKDCASSLELDRRHWPRRAALDRVQEQIAGAFDRWL
                     ERWRRPNSP"
     gene            complement(14995..15978)
                     /locus_tag="VFB37_15670"
     CDS             complement(14995..15978)
                     /locus_tag="VFB37_15670"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZO23938.1"
                     /translation="MRLLLLSGMLVLVALASLGDLAAGMSSGDSPGAPSPMPAEHAAP
                     PVAGTVDPALPLSSATSLLVIAPHPDDESLCCAGVIQRVVRLGGRVSVLWVTNGDASE
                     LDLLVIEKRVFIDPVKMRDLGDRRMQEARNATAILGVAPESQYFLGYPDRGILSLATD
                     NYATPYRSRFTNATSVPYRAALFPGHSYTGQSLESDFGSVLDRVQPTLILAPTPRDSH
                     PDHRATGIMILRVLNRRHELARARYWIVHGGEGWPAPKGFEPGMAMTPAPLQAGLSPT
                     PFVLESIEEQRKLLAIRAYRTQMIVMSTNLLSFVRTNELFSSLPVPWGTVD"
     gene            complement(16030..16800)
                     /locus_tag="VFB37_15675"
     CDS             complement(16030..16800)
                     /locus_tag="VFB37_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO23939.1"
                     /translation="MRLQAVLLTLYVTGALAVPAADLHHKIKLEGRGTPTVILESGLG
                     DTLDVWSAVQPLIAAGCARTLAYNRAGYLGSDPSDAPRDSVTIVGELRAELQRQHIKP
                     PYILVGHSLGGLYMQYFARNYPQEVAGLVLVDSTNWEQHLDPGNQANTPYNSRRAVTL
                     FMPWIVRREISDSLAAGEQVHASPHADVPTVVLSSTRGPAGETPAARATAARLQDEIA
                     ADFPGARHIRVSDSGHYIQHDHPEAVAEAVRKLAGCKP"
     gene            complement(16861..18171)
                     /locus_tag="VFB37_15680"
     CDS             complement(16861..18171)
                     /locus_tag="VFB37_15680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO23940.1"
                     /translation="MTESNCGTGTVAVIGAGIIGCAVAWTLTRSGRRVLLLDRAEPAT
                     AGASFGNVGHIATEQVQPLPSPHLLLHFWRELFAFGGPVDIPARRLITLAPWLGRFAA
                     AAFRQKDNARYFDPLVRGAADALERLLDQVGRRDLLRRHGHYAVWLGPNSVACAAQER
                     HQASTWEVTATPAPPELLRAIAAAAASARPAGLRTPEPAAGLWYPGSAHVLDPAQVAG
                     ALLTGALQKGATLQRADVRSVTPAAKAIEISTEGGTLLVGSAVICAGVWSPPFVAPFG
                     LRAPIEAERGYHVELPDHPPLADAPLMYGDYRVAVTPMASRLRASSYLEFAGLGAAPD
                     PRKPARLRSVLRRLGYQCDPEGPSWMGPRPTLPDYLPAIGRAPGPLDLFYAFGHQHLG
                     LTLSAVTAELIADLLAAREPRFDVSAFDLRRFGSSRGSRLSSSS"
     gene            18241..19146
                     /locus_tag="VFB37_15685"
     CDS             18241..19146
                     /locus_tag="VFB37_15685"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZO23941.1"
                     /translation="MSAPLACNWSGVFPAATTQFDPALDLDLPATRAVQEALIRAGVH
                     GLVILGTVGENNSLSAEEKRAVLQTAVQTAAGRVPVIAGVSEMTTGNAVAYARDATRI
                     GVNGLMVLPAMVYVPTPAELEHHFRSVAQATPLPIMLYNNPPAYRVNIDLQTLERLAA
                     LPNIVALKESAPDSRRFTDLLNAFGERFVLFAGLDDVAFEGLCLGARGWVSGLTNAFP
                     EESLLLYAALRAGDLERARYIYRWFMPLLHLDSEHDLVQSIKLAEQIMGRGSERVRLP
                     RLPLTGARREAVTAMVKEAAARRPH"
     gene            19159..20157
                     /locus_tag="VFB37_15690"
     CDS             19159..20157
                     /locus_tag="VFB37_15690"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF010578.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HZO23942.1"
                     /translation="MKSVFFCIDGHTAGNPVRLVAGGAPFLSGANMSERRQDFLARFD
                     WIRLGLMFEPRGHDMMSGGFLYPPFDPANDAAVLFIETSGCLPMCGHGTMGIVTFALE
                     HGLIRPRTYGRLSLEVPAGLIGVEYTVRSDKVTSVAIRNVPSYLAAEGIRIEVPGLGP
                     LTVDVAYGGNFYAIIEPQGAYTGIDALGASKILTLSPVVRSLMRKAIEPVHPLDSTIR
                     GVSHVMWTDRPKGEGADGRNAVFYGDRAIDRSPCGTGTSARLAHLVARGRLRPGDSYV
                     HESIIGSRFVGRVQQQVDLRGTAAIIPIIEGSAFVTGFNTVWIDPAEPFPTGFQVT"
     gene            20201..20857
                     /locus_tag="VFB37_15695"
     CDS             20201..20857
                     /locus_tag="VFB37_15695"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO23943.1"
                     /translation="MSEPLLRTMSGQITARIREKILTGEYPPGSALLQDVIAAEFGVS
                     KIPVREALVQLRAEGLVDVYAHRGFQVRPLSSTEVDEVFKLRLQIEPAAVADGARQAS
                     AEDLELVRLAFSNLNEALAAGELQESGELNRAFHLALTVPRLQPVTAEILNRLHTLSH
                     RYVRMHLLPHGRVSRATAEHTDLYEAWTSRNAEEARRLTHAHIERTREDLARLLHPPA
                     "
     gene            20906..>21258
                     /locus_tag="VFB37_15700"
     CDS             20906..>21258
                     /locus_tag="VFB37_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO23944.1"
                     /translation="MTRALLPRFHVAFPVNDLSAARRFYGEVLGCPEGRSAERWVDFD
                     FQGHQIVAQLSERPGDPEFNEVDGQNVPVRHFGLILTLAQWRAFTERLKAAEVKFIIE
                     PQIRFQGQVGEQATC"
BASE COUNT         3853 a         6830 c         6936 g         3639 t
ORIGIN      
        1 cagcctggac cgtcctcgac gccgcgacca ctcccgaccg gccgttccgc gcgcggacgc
       61 ggctgaattg cgtgtcggag gaatcctaca gatcgagcgc gaggggagca tagcggggtc
      121 acccacctct agcgcggtgg aggagggttg cccccgttcg gctcgcttgg cgtttggctc
      181 gaattcgcgc cggtctcgcc ataactctta ttgcgcgcct gggcagccga atctccagca
      241 gggtccttgc tctcgccaaa cgtgttgaca tcgccgctgg ccgtcgcggt gctctttggg
      301 ggtggacctg cggggcgcgc ggcagctgga ctcgtggctt gctgcgagca accgagtagg
      361 gagaccatca acagtgcaca ggatgcaccc acaagcatct tcatgagcgt caccttggga
      421 aagccctgaa accgagactc accataccgg tggccgcacc gacgggcagt cggcaaggat
      481 cacatgccgg tggccaggac tccgaacggg ctgtaggagc gggccgactc gactgccgcc
      541 gcgcgcgggg ccggcgcgtg agccgagaag cgcgtgagag tgggcgcggc aacaccaaca
      601 caaggaggcc cgccgggggc gggcctctac aacggactcg tcggaattct tacttgggct
      661 gcgcagactg gtccgtgctg gtgctggcct tgtgatgctt cttcccgtgg tgcttcttca
      721 cgctcttggc cggcgcgctg gctgactggt ctgccggcgg cggcgtgtcg gcggcgaaca
      781 ccgggcccgc gagcaggatc agactcagga cggggacgac ttgcttgaac atcgagaggg
      841 tctccactag aggttgaggg gtcaggcgct gacggcttga gttcctggcc gccgcggccg
      901 ccctattatg acggaaatcg gcccgctttt tcgggcccct catcagccct gcgtcgcgta
      961 gcgggcgcag ctcgagtcag tgcccctcga aggcgaccag ggtgcgcacc ggaaccccga
     1021 gcgcttcgag cttgcgccgg cctcccagct cgggcagatc gatgatgaag cagcccgcga
     1081 ggatctgggc tcccagggcg cgcagcagtc ggaccgcccc ctcggcggtg ccgccggtgg
     1141 cgatcaggtc atccacgaga atgatccgct ctcccggcag gaccgcatcc tggtgcatct
     1201 ccatctcatc gaggccgtac tccagtgagt aggcgacgcg caccgtcgtg tgcggcaact
     1261 tgcccttctt gcggatgggg acgaatccgg cagcgagctg atgtgcgacc gcaccgccga
     1321 gaatgaagcc gcgggcctcg atgcccgcca cacgattgac cttggccgtc gtccagggct
     1381 cgacgagctc gtccaccacc cggcggaagg tgcgcgcgtt gcccagcaaa gtggtgatgt
     1441 cccggaactg gattcccggc ttggggtagt cgggaatcgt gcggatggcg tctttgatct
     1501 cctgaactgt gcccgggtcc accgcctcac tctactccgg tttcctgccc ttggctccag
     1561 cccccctgac gggcgccggc gggcttgccc cagacgaggg tgcgattgtt ggcaggcatt
     1621 gcatgatcgg cgagcagcac caggcccgcc gcgctcgcga gcctctgcac ggcctcgaaa
     1681 tcgcgaatgc cgctgtgcgg gtcacgcgcc cgcagaaaat catcgaaagc agcattgctg
     1741 gtactggtat ggcggccggc gtagcggaaa ggaccgtaga tgcatacgac ggcaggctcg
     1801 accagcgcgg tatccagtcc ccggaagaat tcctggacgc tgctccagct catgatgtgc
     1861 agagtgtttg cactgaagag cccatccaca ggcgctaccc gccagggccg ggcgcgtaca
     1921 tcgagctcga tcggctcgcg caggttcgcc ggcccctgct gagcaatccg cgcgcgcagt
     1981 ccggcgaggt tctcacccac ctcgctcggc tgccaggtga gatgcggaag gtgggcggaa
     2041 aagtgcaggg catgttgacc ggtgccgctg ccgacctcga ggaccgagcg ggtgctggcg
     2101 aaggaagccg tgagaacgcg caggatgggg tgtttgttgc gctcgcaggc ttcagagagc
     2161 gtctgctgtt cgatcgtcaa gggtcagcat cgcgtgggtg acgggcacac aagtctgcca
     2221 cgcgcgcgcg ccgtcggggc ggctgcgtga tcggggctcg catgggagaa tgacgacttc
     2281 cgatcatttg aaggccccca ttccatgcaa gccattgaag cgcttctgca acggcattcg
     2341 gcgcgcgccc tgggcgagcc agcgccggat gatgccgcgc tcgcgctgat gtttcagagt
     2401 gcggcccacg cgcccgatca cgggcgcctg cgcccgtggc gcttcatact cattcgccac
     2461 gacgcgcgcg ggcagcttgg cgagttgctg gccgagcatc tgcgccgcac ccgtcagccg
     2521 atctcggaag aggcgctcga gcgcgaacgg cagaaagcct tccgcgcccc ggtgatcatt
     2581 gccgttgccg ccattatcgc gctcaacgcg aaagtaccgc ccatcgaaca ggtcctctcg
     2641 gctggtgcgg ctgcttatgg cctgatgctc gcggcattct cgctggggtt caacgccatg
     2701 tggaagaccg gcgggccggc gtacgatgag acggtgaagt cggccctcgg actggcgcag
     2761 aaggatgccc ttgtcggctt tctctacgtg ggcaccgagg agcgcagcgc gcagccgccg
     2821 gcgcggcccg catggcaaca gttcgtgcag gagtggcagc gccccagttg attatttcga
     2881 gcgggtgagc agccgcggca gatcgcgcgc cgagacggcg cggctgaaca gaaagccctg
     2941 cgcctcatcg cactgatagg ctcggagggc gcgatgctgc tgcgccgact cgactccctc
     3001 ggcgacgacc ttgagaccta gattgtggcc gagcgcgatg atcgcgcgca cgatcgtcgc
     3061 atcgtccggc tcggtggaca tgtgctcgac aaacgagcga tcgaccttca ggcgatccac
     3121 cggaaagcgc ttgagatacg acaggctcga atagcccgtg ccgaaatcat ccaccgccat
     3181 ctgcacaccg agccgcttga gctcatccag catcgccacc agctggggcg cgtcatgcat
     3241 gaccatgctt tccgtcagct cgagctcgag aaagtgcggg tccaggccgc tgttctgcag
     3301 agcctccgca acggtctgta cgaggctctc ctggcgaaac tgccgtggcg agacgttgac
     3361 tgacacgacg atcggaggca gcccggcatc ctgccaggcc ttgttctgcg cgcacgcggt
     3421 ctgcagcacc cacttgccga tgggtacgat gagacccgtt tcctccgcca gagagatgaa
     3481 gcgtcgcggc gcgatggttc cccgctgcgg gattcgccag cgcagcagcg cttcagcgcc
     3541 cacgatctgg ccggtggcca ggttgacacg cggctggtag cgcagcacga actggttgcg
     3601 ctcgagcgca cgacgcagat gcatctccag ctcgagccgc tcggtcgtga gagcgttgag
     3661 ctcgcgcgtg aagaactgga agttgttgcg gccgctgtcc tttgcgcgat acatggccga
     3721 atctgcatgc ttcaggagcg tcagcgcttc ctccccatct tccgggtgca gggcgacgcc
     3781 gatgctgcag ctgacgagaa actccttctg ctcgatcagc cacggctgcg acaggacgga
     3841 gagcatgtgc gccatgagct ggcgaaccga gtcggggcct ccgtggccgt tgatcagcag
     3901 cacgaactcg tcgccgccga gccgcgccac cgtgtcgcaa tcccgcacgc agctgcgcag
     3961 ccgggccgcc acggttttca acaactcgtc cccgagatga tgaccgaggc tgtcgttgat
     4021 gaacttgaag cggtccacgt cgacgaacgc aactgcgatg cgccgatccg agttcgccgc
     4081 cgtgagcagc gcctgctgca accggtcctg cagcaatgag cggttcgcga gtccggtgag
     4141 ggtgtcatag ttggcctgat gctcgatccg gacctggtag aggcgccgct cggtgatgtc
     4201 ctcgaccgtg ccttcatagc cgatcaggcc gccatcctcg ccgtgcacgg cgcgcgcatt
     4261 ctcggagatc cagatgatat cgccgttctt gcgatagacc tgcgactcga atccggtcac
     4321 cgagccacgc gccttcacga tgtgcatgaa ttcgtcgcgc cggtttttct cgacgtagag
     4381 ctggctgctg atgtcacgca cccacgccat cagctcctcg ggagattcga agccgtagat
     4441 gtgagcgagc gccggattcg catcgaggaa ctgaccatcc gtggtggtgc ggaagatgcc
     4501 ctcgatcgca ttgtcgaaaa gcccgtggta acgccgctcc gcctcgcgca gcgcatgctc
     4561 ggcatgtttg cgcttcgtga tgtcctggat cagcccttcc aaagcgacgg ttttgccgtg
     4621 ctcatccgct acgccggtgc cgcgttccca gacccagcgc gtcgtcccat cggcatgcac
     4681 gatgcggtac tcgagatcga atgcgcggcc ctcttcaacg gcgcaccgga tcgcctcgcg
     4741 tacccacatg cgatcctctg gaagcaggag ctcactgtag gagagcaggg gctcggatag
     4801 cagttcggca gcctgtcggc ctatcacccg cagacagcca ttgctgacga actccatcgt
     4861 ccagtcagcg tccagccgcc gccgatagac catgccgtcc acattggcga gcagggtctt
     4921 gagcatcctc gcgttgtccg gggcggaatc ggcctcggcc ggcgaagacg ggggtttcag
     4981 tgaaagcgcc gtgttcatgg ggaccgtcga tgcgcagcgc tcaacgtatc aatcggcgcg
     5041 tgtttgccgt cagcgcttcg tcacagatgt gacatctgat tggcttgatt tcacataatc
     5101 cgcaaattga gcgcttatgg gtgcccgctc agctctcctc gctgcccgaa gaggcgagcg
     5161 agaccggtcc ggacgggaga ttgggatcgg tagcggcggt ccgctcggcc atgccgtccc
     5221 cgagccgccg ctcgatcaga tgccggatac ggggcggttg ctccccctgg tgaaaggcca
     5281 gcacgcgttg ctcgatctcc tggtccgaga cggtgaagcg atgatggcgg cgcacgtgct
     5341 cgatccagtt ggggctgtga tagcgctcga gccagatctc gggtacggcc agatcctgca
     5401 tgagcgacca gcgcttcgcg ccatcgcggc gccgtatgcg gcgcagctcc ttcatcgcca
     5461 accgaaaccc ctccgtatcc ggtggggcga tgcggtactc cacggtcacc acgaccgggc
     5521 cactgtccat gagtctctcg agctgagcgc gattgaccga ggaccaggtg cgcgagggat
     5581 cgagattgag accttccggc tggcgcagcg gcagccagcg cccgagcaag gtcgaaacga
     5641 ccatgagcac gccggaaagc atcaggcagc gcgccaggcc gaactcgctt gcgacctcac
     5701 ccgagagcca gctgccgatg gcgagtccgc cgaaggtgac catctgatag atcgccacgg
     5761 ttcggccgac tacccaccgg ggcgaggacg tctgaaccgt cacgttgaat gtggagagcg
     5821 ccagaaccca gcaggctccg ccgatcatga gggcaaccat gctcagtgca tgccaggcgc
     5881 tgagtgccgt gacgatgctc gaggcaccga atccggcggt cgagatctgc acgatccgct
     5941 cgttggtgag cctttcgcgc agcgcggcca agcccagtgc gcccagcacc gcgccggtgc
     6001 cgaatgcccc cagaagcaca ccgtaggtga cggcattgcc gccgatgagg tcccgcgcga
     6061 tgagcggcat cagtgcccac accgtgcttc cgaggaggcc gaacacgatc ccgcgcacga
     6121 gcacggtccg gatttccgga gacaggcgcg catagcgcac tcccgccccg atggccatta
     6181 cgacattctc aggcggcagc agcgcagcag gccgaggtct gcgccagagg atcagaacca
     6241 cgatgaggcc gacgtatgac agcgcattag cgaggaacgc tgccggaggt ccggcctgcg
     6301 ctacgatcac accaccgata gccggacccg cggcgcgagc cagattgaac gcgaggctgt
     6361 tcagtgcaac ggccgagggc agatccgctc gcgagacctg ctcgccgacc gaggattgcc
     6421 aggcaggacc atagagtgcc gttccgcagc cgatgaggaa tgtcagggcc agcagcagcc
     6481 agggtgtgat gagtcccagg tagccgatga cactcagtgc gcccgcgacc aacagcatga
     6541 gcgcctgggc gatgatcatg acgaggcggc ggtcccagac gtcggccgtg gcgcccgacg
     6601 gcagcgacag caacatgatc gggagcgtcg tggatgcctg gaccagcgct accatatccg
     6661 ccgaaggcgc aatcgaggtc atcatccagg cagcgcccac tgcctggatg agacttccaa
     6721 agttcgaaat gagactcgcg gtccagatcg ccgcgaagac tggagattga aaaggcgcaa
     6781 aggtggagga gttgtgccag gaggcgggga acagcgcgga caggcggaag cgaaccttca
     6841 agttccggag ccctcagcgg gaatttgcgg cgacctggaa ttctaccccc cgcacgatgc
     6901 ggctgttgcg gtttggcgac atccgggccg ctcaatccag cttgcggtta ccacggtttg
     6961 accacggcca gtactacgat tgcgatcagc agcagtgccg gcacttcgtt gaagaggcga
     7021 taccagcgct gcgaatgtgt gttgcggccg ctgcgcagcg aactcatcag cgcaccacac
     7081 cagcagtgat agccgatcag ggcgagcacg aggacgagct tcagccgcag ccaccctgcc
     7141 tgcaggtagg caggcgaggc gaggatcatc agcacgccaa acaagatggc gagcgccgcc
     7201 ccgatcgtca tgatcccgaa cagccggcgc tccatcacag cgaagcgttc cagccccagg
     7261 ctatcgctgg ccgccgcgtg atagacgaag agccgtggca gatagaacaa ccccgcaaac
     7321 caggtgacga cgaaaacgag gtgaaaggct ttcagccaaa gcatgagcag cgcgaacccg
     7381 ggtgcggtgg ccgccgcata aaatcacgtc gccacgcgtg agtgcaacct tctgaagcgc
     7441 tcagggcccg gggcggccca tgtaccgcca gacgcaggcc gggtaaggta gaggtttcaa
     7501 tcagaagaag agtgtgccga tgacattgcg aaacatcgag atcgcgcagc gcgcgaagat
     7561 gaagcccatt gcccagatcg cccaggagcg gctggggctg ccggaagaca gtctgtcgct
     7621 gtacggccgc tacaaagcca aggtttctct ggactacgtg cgccagctcg acgctcgccc
     7681 cgagggcaag ctcatcctcg tcacggcgat ctccccgacc ccggcaggcg agggcaagac
     7741 caccaccacc gtggggctgg gagatgcgct gaactttacc ggccgcaaga cgctcatctg
     7801 cctgcgtgag ccggcgctgg gcccggtgtt cgggatgaag ggcggcgcgg ccggcggcgg
     7861 ctattcccag gtcgtcccga tggaggacat caacctgcac ttcacgggag atttcgccgc
     7921 cattgcgctg gccaacaatc tgctggccgc tctgatcgac aatcacatcc accacggcaa
     7981 cccgctcggc ttcgatctgc gccgaatcgg catccgccgc gtcgtcgatg tgaacgaccg
     8041 ggcactgcgc gagacggtcg ccggtctcgg cggccccgcc aacggctacc cgcgccagga
     8101 cggcttcgac atcgtggtgg cctcggaagt catggcgatt ttctgtctgg ccaccagcct
     8161 cgccgacctg cgcgagcgtc tggggaagat cgtggtgggc tacacgagtg cgcagaagcc
     8221 gatattggcc aaagacctca aggcgcacgg agccatgacc gtcctgctca aggacgccct
     8281 ggcacccaat ctggtccaga ccctggaaaa caatccggcg ttcatccacg gtggaccctt
     8341 tgccaacatc gcacacggtt gcaactcggt agttgccact cggaccgcat tgaggcttgc
     8401 ggactacgtc gtgacggagg ccggcttcgg tgccgatctc ggcgcggaga agttcgtcga
     8461 catcaaatgt cgcaaggcaa atctgcgccc gtcagcggcc gtgatcgtcg cgacagtgcg
     8521 cgcgctgaag tatcacggcg gagtcgaggt tgccgagctg ggcaaggaga atctcggtgc
     8581 tctcgaggac ggcatctcga accttcggcg gcatgtggag aacgtccgca acaactacgg
     8641 acttgcctgc gtcgtggcga tcaaccatcg agccgaggat acctcccgcg aggtgcagcg
     8701 gctggtggac ctcgtcgagc cttcgggcgt caaagtcgtg ctgggccgcc acttcgccga
     8761 gggcagccag ggggccgtgg acatcgcgaa cgaggtggtc cgtctgtgtg agcagccgag
     8821 cagcttcaag ttcgtctacg acgagcacgc gacgctctgg gaaaagatca gggccatcgc
     8881 gacgaaggtc tatcacgcct ccgacgtcga tgccgatgcg aaagtacgcg cacagatcga
     8941 tcggctgcag cattcgggtt acggacacta ccccgtatgc gtggccaaga cgcagtactc
     9001 gttcagtacc gaccccaagc tgcgtggcgc tccgacgcat cacgtcgtga tcattcgaga
     9061 ggtccggttg gcggccggcg ccgagttcat cgtgatgatc tgtggcgaca tcatgacgat
     9121 gcccgggctg cccgccgtgc cggcggccaa ctcgatcgac atcgacgaga acggaaaagt
     9181 cgtggggctc ttttagtctg atccgcggtg aacggccgta cggccgttca ccgcaggcct
     9241 catgttctcg aagcggtcac gcagcagacg attgccgccc atccgtcaag gctcggcgcc
     9301 cgaaaagcgg gttgcggtgg cgccctctac cggcccgatt tgcgcacggt cgctcacgta
     9361 gagctgcact tcgcgcttga aaatgagcga gccctgcacg accgcatcag gaccgatcac
     9421 gatgcgcggc cgccgccagg agccgaagtg gaaccaccct gcattgtcgt tggtgacgcg
     9481 gatgccgccc tcgatgtgtg aggccccgcc aagggctatg tcaccggagc tcgtctcgat
     9541 gcccccgccc acgtgcgcac tctccaggtg tatggatccg ttcacgttgg tgacgcgccc
     9601 tcccacgtcc gcgccgcgat ccagcaatac ggagccgttg acgacatcga cactgccagc
     9661 gacgcgggcg ccctggttca gatggaccga gccgttgacc gttttcaccg attgcgcgaa
     9721 ggcgtgctcg tggagcttga cgctgccgtt caccgtctcg gcgtccttga cgacacagtt
     9781 ggcgccaagg tcgatggaac cgttgacggt cgtcgcatcg ccggtccgct cccccgcggc
     9841 aatgcgcacc gagccaagaa ccttcgaggt ctcatcaccg cctgcacagc cggcgagcag
     9901 ccccggaccg aaggccagca gtacaagaag aaccactcga atcatggtct cccctccatt
     9961 tcgcaggcgc ccatagtgcg gcgcggcgcc ccacaattca aagtctgttc gagagaggga
    10021 tcagcgggtg ataatcagcc gatgaaaaaa gcctcccggg cgaaaggcag aagaccgcga
    10081 cgcatactcg tactggatgt gggcggctcg cacgtcaagt tccgcgtcgg gccacgcggt
    10141 aagaccggca gattcgtgtc cggtccacac atgaccccgc agaaaatgac ccgtactctg
    10201 ctcgagctgc agccgcgctc cgcttatgac gccgtctcca tcggttatcc aggcctggtg
    10261 ctgcacggaa ggataacggc cgagcctcac aatctgggcc acggctgggt tcatttcgat
    10321 ttcgagaaga ttctcggtgc acccgtacgt gtgatcaatg acgctgccat gcaggccatc
    10381 ggcagctacg cggggggccg aatgctgttt ctcgggctcg gcaccgggat gggtgcgacg
    10441 ctcatactcg agggcgtggt cgagccgttg gagatcggac atcttcccta ccgggatggc
    10501 cactctttcg aggactatgt gggcgagcat ggccgctcgc agctgggcaa caagaagtgg
    10561 cgcaaagtgg tgcgggaggt cgtggcccag ctcagcaagg ctctcgagac ggattatgtc
    10621 gtgatcggcg gcggcaatgc caagcgcctg ctgagcctcc ccaccaactg ccgcctgggc
    10681 gacaatcgca acgcattcgc cggcggattg cgaatgtggc aacactcgag cgatccattg
    10741 cagatcagct cgcaacgaac caggcgccgg aaatccccgg tcaacgctgt ttgatgttag
    10801 ccgttgtagg cgcttagtag cgtgtgctac gcgacccgga tgtgggagac ggcatatgaa
    10861 ggcgattgca acgtgcctcg gagccctcgc ggcgggcctg ttgtgctccg cagcgatggc
    10921 gcagaacgcg cagcaggacc agcagcgtga tgtgaaccaa caggatcgca tcgagcaggg
    10981 gttgaagtcg ggtgagctct caacgcgtga ggcgggtcag ctcgaacggc aacagcagca
    11041 catcgatgga atggaagcca aggatctgaa gaacggctcc atcagcgccg gcgagcaggc
    11101 ccggctcaat tccgccgaga atcgcgccag tcgcgacatc gcagctgaca aacacaacgc
    11161 cgtgaccggc aatgcgggct cggcatcctc cgagcgtatg caggctgatg tgcagcgcaa
    11221 cgtaaatcag cagcagcgca tcgccaacgg catgaacagt ggcgcgctga caaatcgtga
    11281 agcaggttcg ctcgagcgcg gtcaggcaca cgttgaccgt cgtgaggcca atgccgcagc
    11341 caacggtcgc gtcggcgcag gcgagcagcg ccggatccag cggtcgcaga atcgccagag
    11401 tggccggatc taccgcaaga agcacaacct gcgcacgaag tgacgagggg ctcgcggcgc
    11461 tgtctgggac agcgccgcgg ccgagggtcc gtgcgtggct cgcaacctgg cggcgaagga
    11521 cttgcgggca acatccaata tctgcgagtc ttccggtggt atgccattca cccttgcgca
    11581 cccggcagcc gtgctgccgc tgcgccgctt caggttcctg ctgaccgttc cgctcgtcat
    11641 cggcagcgtc gtgccggacg tgccgtacta cttcccccgc cggattgcac agctctggaa
    11701 cgatacccac acactcgacg gctcgttttt catctgtctg ccgcttggca tggctttgct
    11761 cgtagccgtg gtgttgcttc gggatcctct gaccgtgctg ctcagtccgc gtgcccggac
    11821 ggcctgcctg cgatcgatcg aggaattcgc caagcgtccg atgcactggc cgattgcgct
    11881 actgtcgctc ctgatcggga cctggacgca catcggatgg gattcgttca cccaccccga
    11941 cggctggatg accatgcggg tggcagccct cggcgcgccg atctccctgt tcggctggga
    12001 tacgaccatg agccatcttc tgcagtacgt gagctcgata gtcgggctgg tggccgtcgc
    12061 ggtctggatc cggcgcgccg tgcgtcgcac gcctgcgccg gctaatgccg agatcctgcc
    12121 gtcacggctg cgccggactc tcctgctgat ggttacggct tcgacgttgc tgatcgtgag
    12181 catgcttgcc tggcgccagg agtggtaccg ggactcttat tatcatttga gctacctgct
    12241 gctgacgcgc acgatagcct ggttcggcac gctgtacctc ggggcgggac tcgcggtcat
    12301 gttcaaccga cgcgccgtcc ccgagccggt ggcctgagaa tttatcagac cagcaacccc
    12361 gcgatcccga gtgccgtgcc gatcaccgca gcgctcagcc ccagagcggg aaaaaaccgt
    12421 cgacgcgcga ggaagtacaa cgaggagagc accaggccga gctcgaggaa tccttccgcc
    12481 agatccaggc gcagcgctct gcgctcctcg cggcgggtct cctcctccgc agcgcgcgcc
    12541 accttctcga tgtctgcggt ctcctgcgcg tatcgcgcac cctgggcgga gtaacgctcg
    12601 atcagaccct gcaccttgct ctcgtcggct gcgagagtcc gggcgagccc cgcgccaacc
    12661 tccagaacct gctggcggac ttttttagcc tgatagaagg accactgatc gttcgcctcc
    12721 gtccggttga tcacagcctg ggtgtgagcg cgatgcgatg ctatcgtgac gacggcgagc
    12781 aggatgccaa tgacacccac ggcgacgcca acgcgctgtg caaacgtgtc accggcgtga
    12841 tcgtgcccgg tgtgaatctc gacttctgcc atcaacgctc ccagtcatgc gtgctgttgc
    12901 cgcgcgattc tacgagatcg cgtcagtcag cgcggtgttg cgaacacctg cgtccagtaa
    12961 atgcctcctg gactcgacgc gttcacggcg taagcaacgg ccatttggct gaatcccgcc
    13021 gtcatcacat tttcgcaatg atgcgggctc gccagccaac cgctgacggc ctcgtcggcc
    13081 gtcatgactc cgcttgcgag gttctcgccc acaacccgcc accggtagcc ggtgcggctt
    13141 acccgatcag gaggactgct gccgtcgtga cccgtgtgat ccaggtaacc gtgcgcagcc
    13201 atgtccctcg agtgctctag cgcagcagcc tcgagtgcag gagccagcac gagggggctt
    13261 gcggcgggat aaagaacgtt gccgcaccgg tgaggcttgg accgggcaag attcgtgagc
    13321 tcgagaacgc gccgcgcgac caccgcctgc gcttcgggcc gcggtggcag gaaaggtgcc
    13381 gcgagcacga gccacacatc cggcccctgc cggtgggtac cgatttcccg catcgccttg
    13441 tcaagggcct gggagcagaa ccgccggctg acgagcgcct cgacggcgcg atcatccgac
    13501 acatgagtca tgcgtacgaa ggcagaggac acggcgcggt atcccgctcg ctccatcgcc
    13561 gcgtgcccgt tctcaccacg cgccagcagc cgggccgcct gatcgagacg cggattctct
    13621 cgcaagcgcg gagccccgcc gacgctgccc gggcatccgt gcaggcggac gaggttgacc
    13681 gctccggcga ggtcgccaaa ggcctgcagc ggcaacatcg ccactgaagc agcaagcaag
    13741 catgttcgaa acaaagttgg gacctctatg gacgagggta cacggctggg tcatctttgg
    13801 agcatgcccg tctcacgcga atcgaatccc gagaccccta ccgccagctg gccatggcgg
    13861 gagggcaatc gattcctgct gctcgaggca agcgagcagt acctggaacg catggtggct
    13921 gcaatcgatg acgcgcgatc ttatgtcctg ctggagatgt acctcgtcga atcgggggtc
    13981 atagcgggcc gcttcgtgga ggccttggtg cgctcggccc gccgcggcgt gcgcgtgcac
    14041 gtcatcgtgg atgggttcgg ctcgctcgcg ctctcccagg cagatcgtcg cgagttgctg
    14101 gactcgggtg tcgagttgcg tgtctacaat ccattgcgcc tggcgaagcg cttacgcaac
    14161 ttcatacgcg atcaccgcaa gctcatgctc atagatggcg aggtcgcttt tgtagggggc
    14221 atcggtctga ctgacgcgtt tggcgtagtc gggccgccgg gatggccctg gcgcgatctg
    14281 gtcgcagaga tacacggtcc ggtcgtcgca gattggcagc gcgccttcgc gcagacgtgg
    14341 cgcacgagcg gctcgcctct tctcatcccg gttccgacca tcggtatcgt aagtggtgag
    14401 ggcgcacgcg gccgtgtggc cctgagtgag gcctggtatc gctcggagct ggcgagcgcg
    14461 gtcgcacggc gcatcatcga agcacgcggg cgggcctgga tcatgagcgc gtacttcgtg
    14521 ccttcccgcc gttttcgcaa ggctctgcgt cgcgctgcgc ggcgtggcgt agacgttcgg
    14581 ttgttagtgc ccggaccgct caccgaccac ccttgggtgc gccaggccgc gcggcgcttt
    14641 tacggcaagc tgctgcgcaa cggcgtgcgc atcttcgaat tccagccgcg cgtgctgcac
    14701 gggaaaatga ccatctgcga tgactgggtg agtgtcggct ccagcaatct cgatcgctgg
    14761 agcttcaagt ggaatctcga ggcgaaccag gaggtggaaa gcgccgcgct ggcgcaggcc
    14821 gcagccgctg tgttcgagaa ggattgtgct tcgtcgctgg agctcgatcg caggcactgg
    14881 ccgcggcgtg cggccctgga tcgcgtgcag gagcagatcg cgggggcttt tgaccggtgg
    14941 ctcgagcgct ggcgccgacc gaactcgccc tagaaggcag ccgccgcagg cgatctaatc
    15001 gactgtgccc cagggtacgg gaagtgagga gaacagctcg ttcgtgcgca cgaacgacag
    15061 caggttcgtg gacatcacta tcatctgcgt gcgataggca cggatggcca gcagcttgcg
    15121 ctgctcttcg atcgactcga ggacaaaggg tgtcggcgac agccccgctt gcagcggtgc
    15181 gggcgtcatg gccatgccgg gctcgaagcc ctttggcgcg ggccaaccct cgccgccgtg
    15241 aacgatccag tagcgcgcgc gcgcaagctc atggcgccga ttgagcacgc gcaggatcat
    15301 gattccggtc gcgcgatgat caggatgcga atcgcgtgga gtgggcgcga gaatcagcgt
    15361 cggctgcacg cgatcgagaa ccgacccgaa gtcgctttcc aggctctgcc ccgtatagga
    15421 atggccggga aacaaggcag cacgataggg gacgctcgtg gcattggtga atctcgatcg
    15481 atagggcgtg gcgtaattgt ccgtcgcgag agagaggatt ccgcgatcgg ggtagcccag
    15541 gaaatactga ctctccgggg cgactcccag aatggcggtg gcgttgcgcg cttcctgcat
    15601 gcgccgatct ccaagatccc tcattttcac cggatcgatg aacactcgtt tctcgatcac
    15661 gagcagatcg agctcggaag catcgccatt tgtcacccag agcacgctca cgcggccgcc
    15721 gaggcgcacg acccgctgga tcacgcccgc gcagcacagt gactcgtcat cggggtgggg
    15781 agcgatgacc agcagcgaag tcgcactcga gagtggcaaa gcgggatcca ctgtcccagc
    15841 gacgggcggc gcggcgtgct ctgccggcat cggggaaggg gcgcctgggc tgtcgccgga
    15901 actcattccc gctgcgaggt ccccgaggct cgcgagagcc acgagcacca gcatcccgct
    15961 caagagcagc aagcgcaaca gactcccgcc tcctgtagcg gcatcaccca atgcaggcag
    16021 agggcacaat catggcttgc agccggcgag cttccggaca gcttccgcga ccgcttcggg
    16081 atggtcgtgc tggatgtagt gcccgctgtc actcacccgg atgtggcgcg ccccggggaa
    16141 gtcggccgcg atctcatcct gcagacgcgc tgccgtagcc cgagcggccg gagtctcacc
    16201 ggcgggtcca cgggtgctgg aaagcacgac cgtcgggaca tccgcgtgcg gacttgcgtg
    16261 gacctgctcg cccgcggcaa ggctgtcgct gatttctcga cgaacgatcc agggcatgaa
    16321 cagggtgacc gcgcggcgcg agttgtatgg agtgttggcc tggttgccgg gatccagatg
    16381 ctgctcccag ttggtcgagt ccacgagcac cagtccggca acttcctgcg gatagttgcg
    16441 tgcgaagtac tgcatgtaga gtccaccgag ggaatgcccc accagaatgt acggcggctt
    16501 gatgtgctgg cgctgcaact cggcacgcag ctcccccacg atggtcaccg agtcgcgcgg
    16561 agcatcggac gggtcgctgc cgaggtagcc ggcacggtta tacgccaggg tgcgggcaca
    16621 gccggcggca atcagcggtt ggaccgcgct ccagacatcg agcgtatccc ccagccccga
    16681 ctcgagaatg accgtcggcg tgccgcgtcc ctcgagcttt attttgtggt gcagatctgc
    16741 ggccgggacc gccagcgcac cagtaacgta cagggtgagc aggaccgcct gcagacgcat
    16801 ccggggagca tagtcgaggc tcgctgccgt gagccagcga aactgtccac gcccggcggg
    16861 tcagcttgaa ctggacaggc gggagccgcg gctcgagccg aaacgcctca ggtcgaaggc
    16921 cgagacatcg aatcggggct cgcgtgccgc caggagatca gcgatcaact ccgccgtcac
    16981 cgccgacagg gtcagaccca gatgttgatg gccgaaggca taaaacagat ccagcggtcc
    17041 cggtgcccgg ccgatagcgg gcagatagtc cggcagggtc gggcggggac ccatccagct
    17101 cggcccctcg ggatcgcact ggtagccgag gcgacggagt accgagcgca ggcgagcagg
    17161 cttgcggggg tccggtgcgg caccaagtcc ggcgaactcc aggtagctcg aggcgcgcag
    17221 gcgagaagcc attggcgtca cggccaccct atagtcgcca tacatcagcg gcgcatctgc
    17281 gaggggaggg tgatcgggca gctccacgtg atagccccgc tcagcttcaa tcggagcgcg
    17341 tagaccgaat ggagcaacaa acggaggaga ccagacgccg gcacatataa ctgcactgcc
    17401 gaccaaaagc gtaccgcctt cggtgctgat ctcgatcgcc ttcgccgccg gagttacgct
    17461 gcgcacgtcc gcgcgctgaa gcgtcgcgcc tttttggaga gcgcccgtca gcagcgcccc
    17521 tgctacctgc gccggatcca acacatgagc gctcccgggg taccacagcc cagccgcggg
    17581 ctcgggtgtg cgcaggcccg caggccgagc actggccgcc gccgccgcaa tcgcgcgcag
    17641 cagttcgggc ggagcaggcg tggcggtcac ctcccaagtg ctggcctggt ggcgctcttg
    17701 agcggcgcac gcaaccgagt tcggccccag ccagactgca tagtggccgt gccggcgcag
    17761 cagatcccgg cgtcccacct gatcgagcag cctctcgagt gcatcggcgg caccccgcac
    17821 gagcgggtcg aaatatcgcg cgttgtcttt ttgcctgaag gcggctgcgg caaagcggcc
    17881 tagccaggga gccagcgtga tcagacgtcg agccggaatg tcgacagggc cgccgaacgc
    17941 aaaaagctcg cgccagaagt gcaacagaag gtgcggcgag gggagcggct gaacctgctc
    18001 cgtcgcgatg tggcccacgt tgccgaagga ggcacctgcc gtcgccggtt cggcgcgatc
    18061 gagcagcaac acgcgccggc cactgcgagt cagcgtccac gctacggcgc agccgatgat
    18121 tccggcaccg atcacggcga cagtgcctgt gccgcagttt gattccgtca tgcgaggcgg
    18181 ctacccttgt cgcctgccat accgtatacg atctacgata gcacctcaac aggtcccgcc
    18241 atgtcagctc cgctcgcctg caactggtcc ggagtttttc cggccgcgac aactcagttc
    18301 gatcccgccc tcgacctcga tctgccggcg acccgcgccg tgcaggaggc tctgatacgc
    18361 gccggggtcc acggcctggt catcctgggc accgtaggcg aaaacaactc gctttcggca
    18421 gaggaaaagc gcgccgtact tcagaccgcg gtgcagaccg cggccgggcg ggtgccggtc
    18481 atcgccgggg tttccgaaat gacgaccggc aatgccgtcg catatgcgcg cgatgcgaca
    18541 cgcatcggcg tcaatggtct gatggtactg cccgcgatgg tctacgtgcc cacgcccgcg
    18601 gagctcgaac accactttcg cagcgtcgct caggccacgc cactgccgat catgctctac
    18661 aacaacccgc cggcgtatcg ggtgaacatc gatctgcaga cgttggagcg gctggcggcg
    18721 ctacccaaca tcgtcgcgct caaggaatcg gcacccgatt cgcgccgctt caccgatctg
    18781 ctcaatgcct tcggcgagcg cttcgtactg ttcgccgggc tggatgatgt ggccttcgag
    18841 gggctgtgcc tcggagccag gggctgggtg tccggtttga cgaacgcctt cccggaagag
    18901 tcgctgttgc tttacgcggc cctgcgcgct ggcgatctcg agcgcgctcg gtatatctat
    18961 cgctggttca tgccgctgct gcatctggac tctgagcatg atctggtgca gtccatcaaa
    19021 ctcgcggagc agatcatggg ccgcggctcg gagcgagtcc ggttgccgcg gctgcctctg
    19081 acgggggcgc gccgtgaggc ggtcacggcg atggtgaagg aagcggccgc caggcgcccg
    19141 cactagccgg acgggcgcat gaagtcggtc tttttttgca ttgacggcca caccgcgggc
    19201 aatccggtgc gactcgtcgc cggaggggcg ccgtttctct ccggagccaa catgagcgag
    19261 cgccgtcagg atttcctcgc gcgcttcgac tggattcgcc tggggttgat gttcgagccc
    19321 cgcggccacg acatgatgtc gggtggattc ctctatccgc cgttcgaccc cgcgaacgat
    19381 gccgccgtgc tgttcatcga gaccagcgga tgcttgccca tgtgcgggca cggcacgatg
    19441 ggtatcgtga cattcgcgct ggagcacggt ctgatccggc cgcgcaccta tggccggctc
    19501 agcctggagg tgcctgcggg actgatcgga gtcgagtaca cggtccgctc cgacaaggtc
    19561 acctcggttg cgatccgaaa cgtcccgtcc tacctggccg ccgaaggtat tcgcatcgag
    19621 gtcccgggcc tgggccccct cacggtcgat gtggcatacg gtggcaattt ttacgccatc
    19681 atcgagcctc agggcgccta cactggaatc gacgcactcg gagcatcgaa gatcctcacc
    19741 ctgagcccgg tagttcgctc tttgatgcgc aaggcaatcg agccggtgca cccgctcgat
    19801 tccaccatcc ggggcgtcag ccatgtgatg tggaccgatc ggcccaaggg cgaaggcgcc
    19861 gacgggcgta acgcggtctt ttacggtgat cgggcgattg accgatcgcc ctgcgggact
    19921 ggaacttcag cgaggctggc gcatctggtg gcgcgcggac ggctgcgacc gggcgattca
    19981 tacgtgcacg agagcatcat tggcagccga ttcgtgggca gagtgcagca gcaagtggat
    20041 cttcgcggca cagcggctat cattccgatc atcgagggtt ccgcgttcgt gaccggcttc
    20101 aataccgtgt ggatcgatcc tgccgagccg tttcccacgg gtttccaggt gacctgaaac
    20161 gggccgggcg cggcagcgga acgccgcgac atttgcaaac atgtccgaac cactgcttcg
    20221 caccatgagt gggcagatca cggcccggat ccgggaaaaa atcctgacag gcgagtatcc
    20281 gcccggctcg gcgctgctgc aggatgtcat cgcggcagaa ttcggagtga gcaagattcc
    20341 agtacgcgaa gctctcgtgc agctcagggc ggaagggctg gtagacgtct acgctcatcg
    20401 aggattccag gtccggcctc tgtcttcgac ggaggtggac gaggtcttca agctgcgcct
    20461 ccagatcgag ccagccgccg ttgcagacgg ggcccgacag gcctcagccg aagatctgga
    20521 gctcgttcgc ctcgccttca gcaacctcaa tgaggcgttg gccgccggtg agctgcagga
    20581 gtccggcgag ctcaatcgtg ctttccatct ggcgctgacc gtgccgcgcc tgcagccggt
    20641 gaccgccgag atcctcaaca gactgcacac actgtcacat cgctacgtgc gcatgcactt
    20701 gcttccgcat gggcgcgttt cgcgcgcgac ggccgagcat acggacctgt acgaggcgtg
    20761 gacgagccgc aatgcggaag aggcgagacg cctcacacat gctcacatcg aaaggacgcg
    20821 ggaggatctg gcgcgccttc tgcacccgcc tgcctgatcg cacaggcgcg gcctaagtcc
    20881 agtcgcccgc tccagagagg ctttcatgac gcgcgcactc cttccacgct ttcacgttgc
    20941 gtttccggtc aatgatctga gtgcagcgcg ccgcttctat ggcgaggtgc tcgggtgccc
    21001 cgaagggcgc tcggccgaac gctgggtcga ctttgacttt cagggtcacc agatcgtcgc
    21061 ccagctcagc gagcggccag gggatccgga gttcaacgaa gtggacggac agaatgtgcc
    21121 cgtgcgccac ttcggcctga tcctgacgct cgcgcaatgg cgcgctttca cggagcgcct
    21181 gaaggcggcc gaggtgaaat tcatcatcga gccgcagatc cgcttccagg gacaggttgg
    21241 agaacaggcc acctgctt
//
LOCUS       DASRHN010000387         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_965655, whole genome shotgun sequence.
ACCESSION   DASRHN010000387 DASRHN010000000
VERSION     DASRHN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965655"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3611)
                     /locus_tag="VFB37_15705"
     CDS             complement(<1..>3611)
                     /locus_tag="VFB37_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO23945.1"
                     /translation="DPAAIPGWVENLWPLDVAEVSREAGARAAFYAQSDRRAALATQY
                     STLRALLEGSQLSWQVREASEEDDERVAELTRRTNQFNLSKRPLSTPEVADWRLQGRE
                     VWVMGASDRFGDYGTVGAALAHRSGSTLRVEQFVLSCRAMNRGLEYVMVRAIASAARG
                     RGALRIRFPFRASPKNRPARLFVESLAEACRAELLPEGGCELALESIDGPIDALQATV
                     LDRPFAESSDEAEKTPSAVSAEAGQRSSGGQGELLRTLAAQLRDTASIRRLIRRHAQK
                     RDINVDFMPPRDDVQRVIAEVVAQHLNIEQLGVQDDLLALGMDSLRALQIVSAVRKYG
                     YNLRIDDVFEHRTVEALAALCARTGSREGAAAVTPDAGAVAEAAGVAGAAGAAVGFDL
                     AALPISLTEGIEAAYPLSSTQAGMVFHAERDTESPLYQVIDSFKLRGPFEEAAFRRVL
                     SFVTRKHPALRTSFELARFELPMQLVHRHVEIPLEVIDLRGEPAAARNERFDSWLAAE
                     TTRRFDITRPPLLRYTVHLFEDGVFQVTLTQHHAILDGWSLNSLVRELLTTYADVFSG
                     RQLPQPSTPRPAFCDFIQHEREALASAEQREFWRRKLADLQASAIPRWPEHTAAHKIA
                     AEATHVVIPLSVSSALFDTARQAGVPIKSVLLAAHLRVVMYLSGRTDVVTGLLVNGRL
                     EGGDGDRALGLFVNSVPLRARFGGGSWLDLARRCFELEREIQPYRNFPLSEMRKLSPV
                     NPLFEFLFTFTHFHVLQGTRELRQFEVLDTFNFVRDNFPLQAFFDVDPFNGRNIRLML
                     GYDAARIPPVELDRIAALYLELLEEIGSRPGVSCFPPTLPASLSGHLQGKGQPAAFAR
                     AGRASAAPDFESIHRQIARHARERPDKIAVASGDWQLSYAGLHRQAATLAAALRRRNV
                     GIECVVAVALEPSPLAVIAMLGILQAGAAYLPVDPAYPDHRIRAMLEDSGVALLIVDR
                     SGSGLKAGLPDLDIRPALLEDLLVEAAAVDGIAPAVEMPQDAADSVAYVIYTSGSTGR
                     PKGVMISHASIAHLFRVADARFGLDQNDVWSLFHSLSFDFSVWEMWAPLVSGGTLVPI
                     PPLQRLDPQELQQFLIDNQVTVLNLTPSAITRLLMNPGPLRALAGKWCVQRLIAGGEA
                     LPPQLGQELLESGAALWNFYGPTEATVWASAFEVREPRDLEHLGPPLDNCSVDVLSSA
                     "
BASE COUNT          691 a         1198 c         1139 g          583 t
ORIGIN      
        1 gctgagctca gcacgtccac tgagcagtta tcgagcgggg gccccaggtg ttcgagatcc
       61 cggggttcgc gtacttcgaa agcgctcgcc cagaccgtcg cctcggtggg cccgtagaag
      121 ttccacagcg ccgcaccgct ctccaacaac tcctgtccca gctgcggcgg cagagcttct
      181 ccaccggcaa tcaggcgctg gacgcaccac tttccggcga gcgcgcgcag cggcccggga
      241 ttcatcaaca ggcgcgtaat cgcagaaggg gtgagattca gtaccgtgac ctggttgtcg
      301 atcaggaatt gctgcagctc ctgcgggtcg aggcgctgca gaggcgggat tggaacgagc
      361 gtcccgccgc tgaccagcgg cgcccacatt tcccagactg aaaaatcaaa cgacagcgag
      421 tgaaacagtg accacacatc gttctgatcg agaccgaagc gcgcgtcggc aacgcggaac
      481 agatgcgcga tggaggcatg tgagatcatg actcctttcg gccggccggt agacccggag
      541 gtgtagatga cgtaagccac cgagtccgcg gcgtcttgcg gcatctcaac ggcgggcgcg
      601 atcccatcga cagctgcggc ttcgacaaga agatcttcca gaagagcggg ccggatatcg
      661 aggtccggga ggcccgcctt caggccgctg cccgaccggt cgacgatcag tagcgcaacg
      721 cccgaatcct ccagcatggc ccggatccgg tggtcgggat acgccgggtc gacaggcaga
      781 taggcggcac cggcctgcag gatgcccagc atggcgatca cggcgagagg gctcggttcc
      841 agggcaacag cgacaacaca ctcgatcccg acgttccgcc tgcgcaatgc cgctgcaagc
      901 gtcgcagcct gacgatggag ccccgcgtag ctgagctgcc agtcgccact cgcaacggcg
      961 atcttgtctg gccgctcccg cgcgtggcgc gcgatctgac gatggatgga ttcgaaatcc
     1021 ggcgcggccg atgcgcgccc agctctggcg aatgcagcgg gctgcccttt gccctgcaga
     1081 tggcccgaca gacttgcggg caatgtcggc gggaagcagc tcacccccgg acgcgagccg
     1141 atctcttcga gcaactcgag atacagcgct gcgatgcgat cgagctcgac tggaggaatc
     1201 cgcgccgcat cgtagccgag catcagccgg atgttgcgcc cattgaaagg atcgacgtcg
     1261 aagaacgcct gcaacgggaa gttgtcgcgg acgaaattga aggtgtcgag cacctcgaat
     1321 tgccgcagct cgcgcgtccc ctgcagtacg tgaaagtgcg tgaacgtgaa caggaattcg
     1381 aacagcggat tgaccgggct gagcttgcgc atctcgctca gcgggaagtt gcgatagggc
     1441 tggatttccc gctccagctc gaagcaccgg cgtgccagat ccagccagct gccgcccccg
     1501 aagcgcgcgc gcagcggcac gctgttcacg aacaggccga gcgcccgatc gccgtctccg
     1561 ccttccaggc gcccgttgac gagcaacccg gtcaccacgt ccgtgcggcc ggacagatac
     1621 atgacaacgc gcaggtgcgc ggcgagcaac accgacttga tcggcacgcc ggcctgccga
     1681 gccgtatcga atagcgcact gctgacggaa agcgggatga ctacatgagt agcctcagca
     1741 gcgatcttgt gagcagcagt gtgctcaggc cagcgcggga tcgcagaagc ctgcagatcc
     1801 gccagcttgc gccgccagaa ctcacgctgc tcagcggatg ccagcgcctc gcgctcgtgc
     1861 tgaatgaaat cgcagaacgc cggcctcggt gtcgacggtt gggggagctg tcttcccgag
     1921 aaaacatcgg cgtacgtcgt cagtagctcc ctgacgagcg aattgagact ccagccgtcg
     1981 agaatcgcat ggtgctgcgt cagcgtgacc tgaaatacac cgtcttcgaa cagatgcacc
     2041 gtatagcgca gcagcggagg ccgggtgata tcgaaacgcc gcgtagtctc ggccgccaac
     2101 cacgaatcga accgctcatt ccgcgcagcc gccggctcgc cgcgcagatc gatcacctcc
     2161 agtggaattt ccacatgccg gtgcacgagc tgcatcggca gttcgaatcg agccagctcg
     2221 aaagacgtcc gcagggcagg atgcttgcgg gttacgaatg agagcacccg tctgaaagcg
     2281 gcttcctcga agggtcctcg cagcttgaaa gaatcgatca cctgatagag cggtgactcc
     2341 gtatcgcgct ccgcgtgaaa caccatgccg gcctgagtag acgacagcgg gtaggccgct
     2401 tcgattcctt ccgtaaggga aatcggcagc gccgcgaggt cgaagccaac tgccgcgccg
     2461 gccgcgccag ccactccggc tgcctcggct accgcccctg cgtcgggtgt tacagccgcc
     2521 gccccctcgc gcgaccccgt gcgcgcgcag agcgccgcca gggcttcgac cgttcgatgc
     2581 tcgaagacgt cgtcgattcg gaggttgtag ccgtatttcc tgaccgccga gacgatctgc
     2641 aacgcacgca acgaatccat cccgagcgcc agcaggtcgt cctggactcc gagttgctcg
     2701 atattcaagt gctgcgcgac aacttcggct atgactcgct gcacgtcgtc gcgaggaggc
     2761 atgaagtcga cgttgatatc ccgcttctgc gcgtggcgcc ggatgaggcg gcgaatcgag
     2821 gcagtgtccc tgagctgcgc tgccaacgtc cgcagtaact ccccctgacc accgctgctg
     2881 cgctggccgg cctcggcgct tacggccgag ggcgtcttct cagcctcgtc ggagctttcg
     2941 gcaaacggcc tgtccagcac agtcgcctgg agcgcatcga tagggccgtc gatgctctcg
     3001 agcgccagct cacagccgcc ctcgggcagc aactcggcgc ggcatgcctc ggccagactc
     3061 tcgacgaaca gccgtgcggg ccggttcttc ggcgaagcgc ggaatggaaa tcggattcga
     3121 agcgcgcctc gcccgcgagc ggcgctcgcg atggcccgca ccatcacata ctccaggccg
     3181 cgattcatcg cccggcagct caggacaaac tgttcgaccc gcagggtcga gcctgaacga
     3241 tgagccaggg ccgcacccac cgtgccgtaa tcgccgaacc ggtcgctcgc gcccatgacc
     3301 cagacttcac gaccctgaag ccgccagtcg gcgacttcgg gcgtgctcag aggtcgcttg
     3361 ctcaggttga actggttggt gcgccgggtc agctcggcga cccgttcatc gtcctcctcg
     3421 gaagcttccc gcacctgcca ggagagttga ctgccttcca gcagagctcg cagcgtgctg
     3481 tattgcgtcg ctaaagcggc acgacggtcg ctctgcgcat agaaggcggc gcgagcgccc
     3541 gcctccctgg acacttccgc cacatcgagt ggccagaggt tctccaccca gccgggaatg
     3601 gcggccgggt c
//
LOCUS       DASRHN010000388         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1958925, whole genome shotgun sequence.
ACCESSION   DASRHN010000388 DASRHN010000000
VERSION     DASRHN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958925"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFB37_15710"
     CDS             <1..274
                     /locus_tag="VFB37_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23946.1"
                     /translation="NIYVRAERQWRQTWIDSSGSRVDFKGGWNGAAMVLEGAWQDFLA
                     PGKPALVRMTYTRSADGSVRQFGEASDDEGKTWKPTFDFTYRPSKP"
     gene            348..>2878
                     /gene="mutS"
                     /locus_tag="VFB37_15715"
     CDS             348..>2878
                     /gene="mutS"
                     /locus_tag="VFB37_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZO23947.1"
                     /translation="MNDNHLSHTPMMQQYLRIKSQNPDILLFYRMGDFYELFYEDARR
                     AASLLDITLTTRGHSAGQPIPMAGVPFHSVESYLARLVRKGESVAICEQLGDPAKSKG
                     PVERQVVRIITPGTVTDEALLEERQETLAAALAIEHDPHGAAERFGLAWLDLSAGRFT
                     VLQSQGRHALGAELERLRPAELLVSENERDDTLARNGTALRTRPPWYFELASASRLLT
                     DQLGTLDLKGFGADELPLAICAAGALLQYIRDTQKSAVPHIRTLHVEERTDALIVDAA
                     TRRNLEIDVSLSGNTPATLFALIDQCKTAMGSRQLRRWLNRPITDQAPLRERYHAIGA
                     LIDGRRFESVREVLAGIGDIERIIARIALRSARPRDLVQLRTSLGLLPSLRAALVPLD
                     SPLLHALRERINTHADAVQLLAASIAAEPAVFVRDGDVIAARYDVQLDELRQIATHTD
                     EFLLELERRERERTGIAGLRLGYNRVQGFYIEVTRKDAERVPKDYLRRQTVKSAERFI
                     TAELKEFEDRVLSARERALAREKELYEAVLTQLIDHLPAMQATTAALSELDSLASLAE
                     RACALQWTRPQLVSEPCLRIEAGRHPIVERFSCAPFVPNDLQLGAERCMLIITGPNMG
                     GKSTYMRQAALIVLLAYMGSYVPADRATIGPLDRIFTRIGAADDLAGGRSTFMVEMTE
                     AANILHNATPRSLILMDEIGRGTSTFDGLSLAWAVARHIATRLRSFTLFATHYFELTT
                     LAAEVEGCANVHLDATEHGEGIVFLHAVKEGPANRSYGLQVAQLAGVPPEVIDQARRY
                     LEALESQRDRQSARNSGPGAAQAELPLEVPPPAPDRVRAALEALDP"
BASE COUNT          513 a          973 c          904 g          488 t
ORIGIN      
        1 caatatctat gtacgtgccg aaaggcagtg gcgccagacg tggatcgatt ccagcgggtc
       61 gcgggtggat ttcaagggcg gatggaatgg agcggccatg gttctcgagg gtgcatggca
      121 ggacttcctc gctccgggca aacccgctct cgtccgaatg acttacaccc gcagcgcaga
      181 cgggtccgtg cggcagtttg gcgaagcctc cgacgatgag ggcaagacct ggaagccaac
      241 ctttgatttc acgtatcgcc cctcaaagcc gtgaatgcga cactggcccc caaaatgata
      301 gaggtggcca gaagacgtca gtgtactgcc tgcctgcgaa aaagctcttg aacgacaacc
      361 acttatctca cacgcccatg atgcagcagt acctgcgcat caagagccag aatcccgaca
      421 ttcttctgtt ctatcggatg ggagacttct acgagctgtt ctacgaggac gcgcgtcgcg
      481 cggcctcgct cctggatatc accctgacca ctcgaggcca ctcggcgggc cagcccatcc
      541 cgatggcagg cgtccccttc cacagcgtcg agtcctatct ggcgcggctg gtgcgcaagg
      601 gggagagcgt cgcgatctgc gagcagctgg gcgatccggc caaatccaag ggtccggtcg
      661 aacgccaggt cgtccggatc atcacccccg gcaccgtgac cgatgaggcg ctgctcgagg
      721 agcggcagga gacgctcgcc gccgcgcttg ccatcgagca cgacccacac ggcgcggccg
      781 agcgcttcgg gctggcgtgg ctcgatctgt ccgccggtcg cttcaccgtg ctgcagtcgc
      841 agggacgcca tgcgctgggc gccgagctgg agcgcttgag accggcagag ttgttggttt
      901 cggagaacga gcgcgacgac actcttgcgc gcaatggaac ggctctgcgt acgcgtccgc
      961 cctggtactt cgagctcgcc agtgcctcgc ggctcttgac cgatcagctc ggcacactgg
     1021 atctcaaggg cttcggtgcg gacgagctgc ccctggccat ctgcgccgcc ggggctctgc
     1081 tgcagtacat ccgcgacacg cagaagtcgg cggtccccca catccggacg ctgcatgtcg
     1141 aagagcgcac ggatgcgctg atcgtggatg ccgccactcg ccgcaatctc gagatcgatg
     1201 tcagcctatc cggaaacacg cccgcgacct tgtttgcact gatcgatcag tgcaagaccg
     1261 ccatgggctc gcgacagctg cgacgctggc tcaatcggcc gatcaccgac caggcgccat
     1321 tgcgcgagcg ctatcacgcg atcggcgcac tgatcgacgg ccggcgattc gagagcgtgc
     1381 gcgaagtcct cgcaggcatc ggcgatatcg agcggatcat cgcccgcatc gcgcttcgat
     1441 cggcgcggcc acgcgatctg gtgcaactgc gtacttcttt gggactgttg ccctcgctgc
     1501 gagcggccct ggtgccactg gactcccctc tgctccacgc ccttcgggag cgcatcaaca
     1561 cgcacgcgga cgccgtgcag ctgcttgcgg cgtcgattgc ggcggagccg gccgtgttcg
     1621 tgcgagatgg cgatgtcata gcagcgcgat acgatgtcca gctggacgag ctgcgccaga
     1681 tcgcaaccca taccgatgaa ttcctgctcg agctcgagcg gcgtgagcgc gagcgcaccg
     1741 gcatcgcggg actgcggctc ggttacaacc gcgtacaggg cttttacatc gaggtgaccc
     1801 gcaaggatgc ggagcgggtg ccgaaagact atctgcggcg ccagacggtg aaatctgccg
     1861 agcgcttcat caccgcggag ctcaaagagt tcgaagaccg ggtcctgagc gcccgcgagc
     1921 gcgcactcgc tcgggagaag gagctctacg aggccgttct gacgcaactc atcgaccacc
     1981 tgcccgcgat gcaagccacc accgccgcac tctccgagct cgactccctg gcttcgctcg
     2041 ccgagcgcgc ctgtgccctg caatggaccc ggccgcaact ggtcagcgaa ccctgcctgc
     2101 ggatcgaggc cggccgtcac cccatcgtcg agcgattctc ctgcgcgccc ttcgtgccga
     2161 acgatctgca gctcggagct gaacgctgca tgctcatcat caccggtccc aacatgggcg
     2221 ggaaatccac ctacatgcgc caggccgccc tcatcgtgtt gctggcgtac atgggcagct
     2281 acgtgcccgc cgatcgcgcc accatcggtc cgctggatcg gattttcacc cgcatcggag
     2341 ccgctgacga tctcgccggc ggccgctcga ccttcatggt ggagatgacc gaggcggcca
     2401 acatcctgca caacgctacg ccgcgaagtc tgatcctcat ggatgagatc ggccgcggca
     2461 ccagcacctt cgacggtcta tcgctcgcct gggcggtcgc ccgccacatc gccacccgtc
     2521 tgagatcctt tacgctgttc gcgacccact acttcgagct gacgacgctg gcggcggagg
     2581 tggagggttg cgcgaacgtg cacctcgatg ccaccgagca tggcgagggc atcgtgttcc
     2641 tgcacgccgt gaaggagggc cccgccaacc gtagctatgg cctgcaggtg gctcagctcg
     2701 ccggcgtccc gccggaagtc attgaccagg cacgacgcta tctggaggct ctggagtcgc
     2761 aacgggaccg gcaaagtgcc cgtaacagcg gtccgggcgc agcgcaagcg gagcttcccc
     2821 tggaggtccc gccgcccgca ccggaccgtg tgcgcgcagc gctcgaggca ctggaccc
//
LOCUS       DASRHN010000389        11005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2443874, whole genome shotgun sequence.
ACCESSION   DASRHN010000389 DASRHN010000000
VERSION     DASRHN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443874"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFB37_15720"
     CDS             complement(<1..190)
                     /locus_tag="VFB37_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZO23948.1"
                     /translation="MKSLQKGFTLIELMIVVAIIGILAAIAIPAYQDYTVRAQVTEGL
                     NLAGAVKTGVAESFANTGT"
     gene            complement(590..2011)
                     /locus_tag="VFB37_15725"
     CDS             complement(590..2011)
                     /locus_tag="VFB37_15725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZO23949.1"
                     /translation="MSVPAAREQSPGTRPVVLIVDDEPDLLELVSLTLGRMNLETRTA
                     ADINTARRLLKSEQFDLCLTDMRLPDGNGLDLVAWIQEHRATLPVAVITAHGNVESAV
                     RALKLGAFDFVSKPLDLGVLRKLVGSAIRLGDRIDPATTMGSAALRLIGSSAAMEQLR
                     EMIARVARSQAPVHIYGESGTGKELVARLVHETGSRRDGPFVAVNCGAIPTELMESEL
                     FGHKRGSFTGAVADKKGLVQSAEGGTLFLDEVADLPLHMQVKLLRLVQEKTVRPVGES
                     REEAVDVRILSATHKNLVDLVGDGRFREDLFYRINVIELRVPSLRERVGDIPDLADAI
                     LTRLGRRMSIQPPDMSEGAFEVLREYPFPGNVRELENVLERALTLCTSGPILAEHIKV
                     RAVTRASSEPGPAAGAPGATPPPVTVAADGSAALGTQLEDIERDAIIKALERTRYNKT
                     AAAKLLGMSFRALRYRIKKLGIE"
     gene            complement(2008..3648)
                     /locus_tag="VFB37_15730"
     CDS             complement(2008..3648)
                     /locus_tag="VFB37_15730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO23950.1"
                     /translation="MPASAASFPTTSAPSDLAWRVIGLLNLYRVLVPLVLMAVQTFAG
                     PQWVLATSRPRLLLSACIAYFAAAVLLIIARRLEWSSLRIVALVNSSVDAAAIALILY
                     ASGGVASGLGILLVLPVGAMAVLASHRDALLIAAVAAIAVLIQQILLDLQEAAPATDY
                     TTAGVLGAVLFVFALCAWPIANRLRESEALVRRKDLDLANLSQLSQYIVQHLRESILV
                     IDTQERIRLINESAAQILGDRHAFPEALVGEASPRLLYLLETWRQNEGNTGIFPPPDQ
                     TFVAADGARVIRAHFAPLGSATPAPVLVFLEDTSLLAEKVQQSKLAALGRLSASIAHE
                     IRNPVGAMSHAGQLLGESPNLSTEERRLTEIMRTNADRVSSIINNVLRLSRRENTHAE
                     TLSLSAWAEEFQEEFCETMQLPPERLHTTETVDSIEVRADPSQLRQIVWNLCENALKH
                     GLSTAPEGVVDIQLGRLSANGRPYLQVMDRGAGVKPEHAEKIFEPFFSAGHGTGLGLF
                     LARELAETNGATLLYEPRHGGGSIFRLVFADLRRWEIG"
     gene            3899..5101
                     /gene="sucC"
                     /locus_tag="VFB37_15735"
     CDS             3899..5101
                     /gene="sucC"
                     /locus_tag="VFB37_15735"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZO23951.1"
                     /translation="MNLHEYQAKEVFRSYGIPIPTGAVAANPEEAVAAAQKLGGSVWV
                     VKAQVHAGGRGKAGGVKLVKDLDAVRSAAEAMLGHPLVTKQTGPEGLPVERVYVESGS
                     QIEREIYLSLTLNRERGRVALIASAAGGMDIEEVAATTPERILTVNIHPAAGLQPYEC
                     RQIAFGLGLQGAQIGEFQNICMGLYRLYMEKDASLVEVNPLIVTKGGGLVALDAKINA
                     DANAIFRHPDIGRMRDTSQEDPMERRASEHDLNYVSLDGDIACMVNGAGLAMATMDLI
                     KLHGGQPANFLDVGGGATSERVTAAFQLILSNTRVRAVLVNIFGGIVRCDLIADGVIN
                     AVKNVGVKVPVVVRLEGTNADKAREMLAKSGLTIAAAEDLTDAARKVVAAAKSGKQST
                     PTKSKSHS"
     gene            5098..5958
                     /gene="sucD"
                     /locus_tag="VFB37_15740"
                     /pseudo
     CDS             5098..5958
                     /gene="sucD"
                     /locus_tag="VFB37_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            6254..7882
                     /locus_tag="VFB37_15745"
     CDS             6254..7882
                     /locus_tag="VFB37_15745"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZO23952.1"
                     /translation="MSKSFRVALAQLDMLVGDVQGNAMRVIDAARRAHAEHQADLVIY
                     PELTLSGYPPEDLLFHRGFRRQIEAGLQKVRQEVQGPAVLIGFPEYAGAAIYNSAALI
                     SGGTTVAIHRKAELPNYKVFDEKRYFEAGAQPTVISTAGVRTGLLVCEDIWTPEAVQL
                     ARADGAQVLITINASPFEIHKQREREMVARERVLDAALPLAYVNMVGGQDELVFDGNS
                     FVMNAQGDVVMRAPAFREGIYLVEFAVESDRRIVPRPAHIEPESTDEASVYAALVLGV
                     RDYVNKHKFPGVVMGLSGGVDSALTLAIAVDALGAERVHAVMMPSRYTASMSLEDAKE
                     QVRILGVKYSVLSIETMFEATLATLKQEFAGLPADATEENIQSRCRMLLLMGISNKTH
                     RMLLTTGNKSEMAVGYATLYGDMAGGFAPIKDCSKMLVYRLATHRNSLSRVIPQRVID
                     RPPSAELRPDQKDSDSLPPYEVLDAILEAFIEEDLSVDEIEARGFERATVGRVLDMVK
                     RNEYKRRQAPPGVRVSRRAFGRDWRYPITNGYRR"
     gene            complement(7984..8886)
                     /locus_tag="VFB37_15750"
     CDS             complement(7984..8886)
                     /locus_tag="VFB37_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZO23953.1"
                     /translation="MERSPVGRLGRERSACPILANFTRREANERPSILFSLPGRFAHA
                     AALALCMILCATAGCRTHRVDATKSSPELLYKKAKKSLDSYDFQTAIKTYEALTARFP
                     FTDQARQARLDLIYAYYRAGEGESATDAADTFIRENPTHPRVDYAWYLKGLVDFDKPP
                     NIFEHLFRVDLSQRPPSTARKSFSAFRTVVEQYPKSAYAHDARQRMIYLRDRLANYEL
                     HVARYYLKRGAYVAAAQRAKACIEQFDGAPAVPEALQIMIQAYDRLNLTPLAEQTRKV
                     YEANYSGQIRDVQAEIKKPWWKIW"
     gene            8885..9880
                     /gene="rluD"
                     /locus_tag="VFB37_15755"
     CDS             8885..9880
                     /gene="rluD"
                     /locus_tag="VFB37_15755"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: protein motif:HMM:NF008385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HZO23954.1"
                     /translation="MSRIPTDPDVSEFRTHSLHVPAEYSGLRLDQALSRLLPQYSRAR
                     LQGWIEAGGVQVDGRALRGKDRVVGGERVQVEAQIEPQKALIAEAIPLDIIFRDRALL
                     VLNKPAGLVVHPGAGNAQHTLQNALLGLDAKLALVPRAGIVHRLDKDTSGLLVVARTP
                     ETHAALVSALSAREIGRQYIALCTGVMTGGGTIDEPIGRHRSQRTRMAVRGDGRPAVT
                     HYRVLERFRGHTLVRAELESGRTHQIRVHFAHVGYPLVGDPVYGGRRRIPAGSSARLV
                     TELNTFKRQALHAARLSLTHPVSGREMSWEAPLPADMTALLEAMKLDSRQQVAER"
     gene            9886..10626
                     /gene="pgeF"
                     /locus_tag="VFB37_15760"
     CDS             9886..10626
                     /gene="pgeF"
                     /locus_tag="VFB37_15760"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZO23955.1"
                     /translation="MILTPEWPAPPQVRAAFTLRSGGVSQAPYDSLNLGTRVGDAAEA
                     VAENRRRVRKQLSLPGEPTWLHQVHGTNVLDLPASAAADTPADGAVCTRSDAVCAVQV
                     ADCMPVLFADRAGTVVGAAHAGWRGLCAGILESTVHKMGAQTAHLLTWLGPAIGPDHF
                     EVGDEVRAAFLAEDPGADAAFVANSRGRWQCDLYALARRRLARLGITAVYGGGWSTWA
                     DARRFFSFRRDGVCGRMAALIWIEADRA"
     gene            10623..>11005
                     /locus_tag="VFB37_15765"
     CDS             10623..>11005
                     /locus_tag="VFB37_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZO23956.1"
                     /translation="MSLLGLIIVFTAVGGIASAAFAGLFLLVPEQTGLRMLPHFISFA
                     TGALLGAALLALLPEAIETVGPGGAHGLGLALVTGFGVFFLIEKLVLWRHAHSHDGEG
                     GGHTAEAHAGEVHTAGAHPHSGHGHS"
BASE COUNT         1955 a         3448 c         3708 g         1894 t
ORIGIN      
        1 aagtgcccgt gttggcgaag gactcggcga cacctgtctt cacggcaccg gccagattca
       61 ggccttccgt cacctgcgcg cggaccgtgt agtcctggta ggccgggatc gcaatcgcgg
      121 ccagaatgcc gatgatcgcg acgacgatca tcaactcgat gagggtaaaa cccttttgga
      181 gagacttcat gtgaaacata cctcgagagg ttgtgagtaa ggcagcggcg cagagcgcct
      241 gcggctgaag caaagcaaag cctgtgccag ggcgcttggt cgcgtgcaag ttattgaaag
      301 caaagtgaaa taataaaaat tgaaacaacc tgcatttgac ttacgtgcgt cagtctccga
      361 cgacagcgtc tgacgggagc gtcacaaact gacgcgccgc gtcatgcgag cggatccctc
      421 accgcactac aacaactacg ggtctacggg gcaggttggt tcgcagctat cgtgatcgac
      481 tgtgatgatg ttttgttgag tcggatcgcc gtgacgcagt tcacagtttg catcacgacc
      541 gatcgaagac gcgactgttg cctagtgcga agtcggtcct aactgtcgat tactcgatcc
      601 ccagcttctt gatccgatag cgcagcgcgc ggaagctcat gcccaggagc tttgcggcgg
      661 cagtcttgtt gtagcgcgtt ctttccagag ctttgatgat cgcgtcgcgt tcgatgtcct
      721 cgagctgcgt gccgagagca gctgagccgt ctgcggcgac cgtgaccggc ggcggggtgg
      781 ctccgggggc gccggcggcc gggccgggtt cagaagaggc gcgggtaacg gcgcgaacct
      841 tgatgtgctc cgccaggatc gggcccgacg tgcacagggt gagggcacgc tccagcacgt
      901 tctcgagctc gcgcacgttg ccgggaaacg ggtattcgcg cagcacctcg aaagcccctt
      961 cgctcatatc ggggggctga atgctcatgc ggcgcccaag tcgggtaagg atggcatccg
     1021 cgaggtccgg gatgtcgcca actcgctctc gcagggaggg gacccggagc tcgatcacgt
     1081 tgatacggta gaagaggtcc tcgcggaagc ggccatcgcc caccaggtcg accaggttct
     1141 tgtgcgttgc ggaaaggatc cggacgtcca cggcctcctc gcgagactcg ccgaccgggc
     1201 ggacggtttt ctcctgaacc aggcgcagca gcttcacctg catgtgcagc gggaggtcgg
     1261 cgacttcatc caggaacaac gtcccgcctt cggcgctctg cacgaggcct ttcttgtcgg
     1321 ccacggcgcc agtgaagctg cccctcttgt gaccgaacag ctcgctttcc atgagctcag
     1381 tcgggatcgc gccgcagttc accgcgacga agggcccatc tcggcgcgag ccggtctcgt
     1441 gaacgagccg tgccaccagc tccttgccgg ttcccgactc gccatagata tgcacggggg
     1501 cctgactgcg cgccacgcgc gcgatcatct cccgcagctg ctccatcgcc gcggagctgc
     1561 cgatgaggcg cagggcggcg ctgcccatcg tggtggccgg gtcgatgcgg tcaccgagcc
     1621 ggatggcgct gcctaccagt ttgcgcagca cgccgagatc cagaggcttg gatacgaagt
     1681 cgaaggcacc gagcttcagt gctcgcaccg cggactcgac gttgccgtgg gcggtgatga
     1741 cggccacggg gagggtggcg cggtgctcct ggatccaggc cacgaggtcc agcccattgc
     1801 catccggcag ccgcatgtcg gtcaggcaca aatcgaactg ctcggatttg aggagccgtc
     1861 gcgccgtatt gatgtccgcc gccgtgcggg tctcgagatt catgcggccg agggtgaggc
     1921 tgacgagctc gagtagatcc ggctcgtcgt cgacaatcag gacaacaggg cgcgtgcctg
     1981 gactctgctc tctggcagcc ggaacgctca tccgatctcc cagcgccgca ggtcggcaaa
     2041 cacgaggcgg aaaatgctgc ccccaccgtg tctgggctcg tagagaagcg ttgcgccgtt
     2101 ggtctcggcg agctcgcgcg cgagaaacag tccgaggcct gtgccgtgcc ctgcgctgaa
     2161 gaagggctcg aagatcttct ccgcgtgctc cggcttcacc ccggcgcccc ggtccatgac
     2221 ctgaaggtag gggcggccgt tggcgctcag gcgaccgagc tgaatatcga ccactccctc
     2281 cggagctgtg ctcaagccat gcttgagagc gttctcgcac aggttccaca cgatctgacg
     2341 cagctgggag ggatcggcgc gaacctcgat ggaatcgact gtttcagtgg tgtgcagccg
     2401 ctcgggcggc agctgcatcg tctcgcagaa ctcctcctgg aattcctcgg cccaggcgga
     2461 cagtgacagc gtttcggcgt gggtgttctc gcgccgcgac aagcgcagca cattgttgat
     2521 gatcgagctc acgcgatcgg cattggtccg catgatctcc gtcagccgcc gttcttcggt
     2581 gctcagattg ggagactcgc cgagcagctg tcccgcatgg ctcatggcgc cgacgggatt
     2641 ccgaatctcg tgcgcaatgc tcgcactcag acgtcccaac gccgccagct tggactgctg
     2701 cactttctca gccagcaggc tggtgtcctc caggaacacc aaaacgggcg ccggcgtggc
     2761 gctgccgagg ggagcgaaat gcgcccggat cacccgcgcc ccgtccgctg ccacgaatgt
     2821 ctgatccggc ggcggaaaga taccggtgtt gccttcgttt tgccgccagg tctcgagcag
     2881 gtacagcaat cggggcgagg cctcgccgac cagcgcctcg ggaaaggcat ggcgatcgcc
     2941 caggatctgg gcagccgact cgttgatgag ccggatgcgc tcctgggtgt cgatcacgag
     3001 gatactctcg cgcagatgct gaacgatgta ctgggagagc tgggagagat tcgccaggtc
     3061 cagatccttg cgccgcacga gggcttcgct ctcgcgcagt cgattggcga tcggccaggc
     3121 acaaagcgcg aacacgaaga gcacggcccc gagaacaccc gccgtcgtgt agtcggtcgc
     3181 gggggcggcc tcctgtagat cgaggaggat ctgctggatg agaacggcga tggccgccac
     3241 cgcggcgatc agcagcgcat cccggtggga ggcgagcacc gccatggcgc cgacgggtag
     3301 caccagcaga atgcccagac cgctggcgac accgccgctg gcatagagga tcagggcaat
     3361 ggccgccgca tcgacgctgg agttgaccag ggcgacgatg cgcaggctcg accattccag
     3421 gcgccgggca atgatgagca gcacggcggc cgcgaaatag gcgatgcaag cggagaggag
     3481 cagccgtgga cggctcgtgg cgagcaccca ttgaggcccg gcgaaggtct ggacggccat
     3541 cagcacgagc gggacgagca cccgataaag attgagcaga ccgatgaccc gccaggcgag
     3601 atcgctgggt gcggaggtcg taggaaagct cgcggcgctg gcgggcatgc tcaaacgcgc
     3661 aagactaggc tggcgcgagc tgcgccggcg gttttgtgtt ctggctcatc ggaccccgga
     3721 ccgttcggcg gtgcgcccct cgaaaaggcg ggcctgctgg caagcgcgac tgtaccatct
     3781 tggcgcaacc tgaaaagcga tgtgcttcgc aggtcgcccc cttgctggtc agtaccggcg
     3841 aatttcgcga aaatatcgat ttttcgcagc ccatgccggg tgcgcgagag gcgagacgat
     3901 gaatctgcac gaatatcagg cgaaagaagt ctttcgcagt tacggaattc ccatccccac
     3961 cggcgccgtg gccgccaatc ccgaggaggc cgttgccgca gcgcagaagc tgggcggctc
     4021 ggtctgggtc gtcaaggcac aggtgcacgc cggcggccgt ggcaaggctg ggggggtcaa
     4081 gctggtcaag gacctcgatg cggtacggtc cgctgccgag gcgatgctag gtcatccgct
     4141 ggtcacgaag cagaccggtc ccgaagggtt gccggtcgag cgggtgtacg tggaatccgg
     4201 ctcgcagatc gagcgggaga tctacctctc cctgaccctg aaccgcgagc gcggccgagt
     4261 agccctgatc gcctccgcag cgggcgggat ggacatcgaa gaagtcgccg ccacgacccc
     4321 tgagcgcatt ctgacggtga acattcaccc cgccgccggg ctgcagccct acgagtgccg
     4381 gcagatcgct ttcggcctcg ggctgcaggg cgcccagatc ggtgagttcc agaacatctg
     4441 catggggctg tatcggcttt acatggagaa ggacgccagt ctcgtcgagg tcaacccgct
     4501 gatcgtcacc aagggcggcg gcctggtcgc gctcgatgcc aagatcaacg ccgatgccaa
     4561 tgcgatcttc cgccaccctg acatcggacg gatgcgggat acgagccagg aagatcccat
     4621 ggagcggcgc gcgagcgagc acgatctgaa ctatgtctcc ctcgatgggg acatcgcctg
     4681 catggtcaac ggcgcgggcc tcgcgatggc caccatggac ctcatcaagc tgcacggcgg
     4741 ccagccggca aactttctcg atgtgggcgg cggagcgacc agcgaacggg tgaccgccgc
     4801 attccaactc attctgtcca atacccgggt ccgcgcggtc ctcgtcaaca tctttggcgg
     4861 cattgtgcgt tgcgatctca tcgccgatgg cgtgatcaac gcggtgaaga atgtcggcgt
     4921 gaaggttccg gtggtcgtcc ggctcgaggg caccaatgcg gacaaggccc gcgagatgct
     4981 tgccaaaagc ggactgacga tcgccgcagc cgaggatctc acggatgccg ctcgcaaggt
     5041 cgtcgcagcg gcgaagagcg gcaagcaaag caccccgacc aagtcgaaga gtcactcatg
     5101 agcatcctca tcaatcgcaa cacccgggtc atctgccagg gttttaccgg caaacagggc
     5161 acattccact gtgagcaggc gctggcctac ggcaccaagc tcgtgggggg cgtcacgccg
     5221 ggacgtggcg gacagactca tctgggcctg cccgttttca acactgtcca cgaagctgtg
     5281 cagcgcaccg gcgccacggc cagcgtgatc tacgttcctg cagccggagc ggccgattca
     5341 atactcgaag cgatcgatgc cggcatcgag ctcgtcgtgt gcatcaccga gggcctgccc
     5401 gtcaacgaca tggtgcgcgt caaagccgtg ctcgcgggct ccggcactcg cctggtcggc
     5461 cccaactgtc ccggcgtcat taccccggag gagtgcaaga tcggcatcat gccgggcttc
     5521 atccacaaga aaggctcggt tggaattgtc tcgcgctccg gcacgctgac atacgaggcg
     5581 gtctttcaaa cgacgcacat cggtctgggc cagagcacct gcgtcggcat cggcggcgat
     5641 ccggtgcgcg gcatgaattt catcgacgtg ctcgagctct tcgagcgcga tccgggcacg
     5701 gagggcatca tcctcgtcgg cgagatcggc ggcagtgacg aggaggccgc cgcggccttt
     5761 atccggcagt acgtctccaa gcctgtcgtc gcctacatcg ccggcgtgac cgctcctccc
     5821 ggcaaacgca tgggccatgc cggagcgatc gtggccggcg gcaaaggcac cgcagcggat
     5881 aagttcgccg cgttcgaggc ggcgggcgtg cgcactgtga agtcaccggc ggacctgggc
     5941 aaggcgatga gtgagctcct cggtcatcgc cgtaaagtcc acgggacggc cgcgggcaga
     6001 actgcccgag gtagccgagc cacaacggtc agcgctcgag cactccctgc gcgcagcccc
     6061 caaggcgcct cgacaaaacc caaggcgaaa cggcctgcat caacggtcgg ccgcaagcgg
     6121 gccgcggcag ctggcgcgca caagcgtccc gcggccaaag ctgctcgcgt ggcgcggaca
     6181 cgcaaacgga cggcgaaaaa gggcggccgc tccgccggac ggcgacgcag gagcgcctga
     6241 cggcctgtag gccatgagca agtctttccg ggtcgcactc gcccagctcg acatgctggt
     6301 cggtgacgtg cagggcaacg ccatgcgcgt gatcgacgcg gctcgccgcg cgcatgccga
     6361 gcatcaggcc gacctcgtga tctatcccga gctcacgctg tcaggttatc cgccggaaga
     6421 tctgctgttt caccgggggt ttcgccgcca gatcgaggcc ggcctgcaga aggtgcgcca
     6481 ggaagtgcag ggccctgcgg tcctgatcgg atttccggaa tacgcaggcg ctgcgatcta
     6541 caattcggcc gccctgatca gcggcggcac gaccgtcgcg atccatcgca aggccgagct
     6601 gccgaactac aaagtcttcg acgaaaaacg gtatttcgag gcgggcgcgc aacccacggt
     6661 catctccacc gcaggcgtgc gcacgggcct gctggtctgc gaagacatct ggactccgga
     6721 ggccgtgcag ctggcgcgcg ccgatggcgc gcaggttctg atcaccatca acgcatcgcc
     6781 ttttgagatt cacaagcagc gcgagcgcga gatggtggct cgcgagcggg tgctcgatgc
     6841 cgctttaccg cttgcgtacg tcaacatggt aggcggccag gatgagttgg tctttgacgg
     6901 caactccttc gtgatgaacg cacagggtga cgtcgtgatg cgcgccccgg cattccggga
     6961 gggcatctac ctggtcgaat tcgccgtgga gtccgatcga cgcatcgtgc cgcgccccgc
     7021 tcacatcgaa ccggaatcga ctgacgaggc gagcgtctat gcggcactcg tcctcggtgt
     7081 gcgcgactac gtcaacaagc acaagttccc gggcgtcgtg atgggattgt ccggcggggt
     7141 ggactcggcc ctgacacttg cgattgccgt ggatgcgctc ggtgccgagc gcgtgcacgc
     7201 ggtgatgatg ccctcgcgat acaccgcatc gatgagcctg gaggatgcca aggagcaggt
     7261 gcgcattctc ggcgtgaaat acagcgtgct gtcgatcgaa accatgttcg aggcgaccct
     7321 cgccacgctg aagcaggagt ttgcgggcct gcccgccgat gccaccgagg agaacatcca
     7381 gtcgcgctgc cggatgctgc tgctcatggg catctccaac aagacgcacc ggatgctgct
     7441 cacgacgggg aacaagagcg agatggccgt gggttatgcc acgctctacg gcgacatggc
     7501 cggcggcttc gcgcccatca aggactgcag caagatgctg gtgtatcggc tggccacgca
     7561 ccgtaactcg ctgagccgtg tgattcccca gcgggtcatc gaccggccgc cctcggctga
     7621 gctgcgtccg gaccagaagg actccgactc gctcccgccc tacgaagtgc tggatgcgat
     7681 cctcgaggca ttcatcgagg aggatctgtc ggtggacgag atcgaggcgc gaggcttcga
     7741 gcgcgccacg gtgggacgtg tgctcgacat ggtgaagcgg aacgaataca agcgccgcca
     7801 agcccctcct ggggttcgcg tcagccgccg tgcgttcggc cgggattggc gttacccgat
     7861 caccaacggg tacagacggt aacggtgcgc ccttgagatc tgaatgtggg gttgcccccg
     7921 ggaagagcgc tccggcgcaa gggggctgcc ccacattcag atctcaagga cactcccccg
     7981 cgcctaccag atcttccacc agggtttttt gatttcggcc tggacgtcgc ggatctgacc
     8041 gctgtagttc gcctcgtaga ccttgcgggt ctgctcggcc agcggggtca ggttcaaccg
     8101 gtcgtaggcc tggatcatga tctgcagcgc ctcagggacg gcgggcgcgc catcgaactg
     8161 ctcgatgcag gccttggccc gctgggcggc ggccacataa gcgccgcgct tgaggtagta
     8221 acgcgccaca tgcaactcgt agttggccag gcggtcgcgc agatagatca tccgctggcg
     8281 tgcatcgtgg gcgtaggcgc tcttcgggta ctgctcgacc acggtgcgga acgcactgaa
     8341 agacttgcga gcggtgctgg gcgggcgctg tgacagatcg actctgaaca gatgctcgaa
     8401 gatattcggg ggcttgtcga aatccaccag ccccttgagg taccacgcgt agtccacgcg
     8461 cggatgcgtc gggttctcgc ggatgaaggt atcggccgca tcggtggccg actcgccttc
     8521 gccagcacgg taatatgcgt agatcagatc gagccgcgcc tggcgggcct ggtcggtgaa
     8581 ggggaagcgg gcggtcagcg cctcgtaggt cttgatcgcc gtctggaaat cgtacgaatc
     8641 gaggctcttt ttcgccttct tgtacagcag ctccgggctc gacttggtgg catcgacccg
     8701 atgcgtgcga cagccggccg tggcacacag gatcatacag agcgcaaggg cagcggcatg
     8761 cgcaaaccga ccgggcagag agaacaaaat tgagggcctt tcgttggctt cgcgccgtgt
     8821 aaagttggca agtatagggc acgcggaccg ctcacgtccg agccggccta cgggcgatcg
     8881 ctccatgagc aggattccga ccgatccgga cgtatcggag ttccgtacgc actcgctgca
     8941 cgtgccagcg gagtattccg ggctgcgcct cgaccaggcg ctctcccggc tgctgccgca
     9001 gtactcgcgc gcccgcctgc agggatggat cgaggcgggc ggcgtgcagg ttgatggccg
     9061 tgcgctgcgc ggcaaggacc gggtggtggg cggcgagcgg gtgcaggtgg aggcgcaaat
     9121 cgagcctcag aaagcgctca tcgcggaggc aatcccgctc gatatcatct tccgcgaccg
     9181 ggccctgctg gtcctcaaca agcctgcagg actcgtggtg cacccgggtg ccggcaatgc
     9241 ccagcacact ctgcagaacg cgctgctggg cctcgatgcg aagctggcgc tcgtgccacg
     9301 cgccggcatc gtccatcgtc tcgacaagga cacgagcggg ttgctcgtgg tggcgcgtac
     9361 gcccgaaacg catgcggccc tcgtttcggc gctgtcggcg cgggagatcg ggcgccagta
     9421 catcgctctg tgcaccggag tgatgacggg cggcggaacg atcgatgagc ccatcgggcg
     9481 gcatcgcagt cagcgcaccc gcatggccgt tcgtggcgat gggcggcccg cggtcacgca
     9541 ctatcgggtc ctcgagcgct tccgcgggca taccttggtg cgggcggagc tggagagtgg
     9601 gcggacccat cagattcggg tgcactttgc gcatgttggg tatcccctgg ttggagatcc
     9661 ggtgtacggc gggagaaggc gcatcccagc cggcagcagc gcaaggctcg tgaccgaact
     9721 caacacgttc aaacgccagg ccctgcacgc cgcccggctc tcgctcaccc acccggtctc
     9781 ggggcgcgaa atgagctggg aggcgccgct gcccgccgac atgaccgcac tgctcgaggc
     9841 gatgaagttg gattcgcggc aacaggtcgc cgagcggtga gctccatgat cctgactcca
     9901 gagtggccgg cgccgccgca ggtgcgcgcg gcgttcacgc tgcgctcggg tggggtgagc
     9961 caggctccgt atgactcgct caatcttggc actcgagtcg gcgatgccgc cgaggcggtt
    10021 gcggaaaacc ggcggcgcgt tcgcaagcag ttgagtctgc cgggcgagcc gacgtggctg
    10081 caccaggtcc acggcaccaa tgtgttggac cttccggcat cggcagcagc ggataccccg
    10141 gccgatggcg ccgtttgcac ccgcagcgat gcggtctgcg ccgtacaggt ggcggactgc
    10201 atgccggtgt tgtttgcgga ccgcgcggga accgtggtgg gggctgcgca cgcgggctgg
    10261 cgcgggttgt gtgccgggat actggagtcc accgttcaca agatgggtgc gcaaaccgcc
    10321 cacctcctga catggctagg ccccgccatt ggcccggacc atttcgaagt tggagacgag
    10381 gtgcgtgctg cctttctcgc cgaggacccc ggcgccgatg cggcatttgt cgccaactca
    10441 cgcggccggt ggcaatgcga cctctatgct ctggcacggc ggcggctcgc gcgactcggg
    10501 atcaccgcgg tttacggtgg aggttggtcc acctgggcag atgcccggcg gttcttctcg
    10561 ttccggcgcg atggagtctg tgggcgcatg gcggcactca tctggatcga agccgaccgc
    10621 gcatgagcct cctcggactc atcatcgtgt ttaccgcggt cggcggcatc gcgagcgcag
    10681 ctttcgccgg gttgtttctg ctcgtgcccg agcagacggg gctcaggatg ctgccccact
    10741 tcatcagctt tgccacgggc gctctgctcg gcgcagcgct gctggcgctc ttgcctgagg
    10801 cgatcgagac cgtgggcccc ggcggggctc atggtcttgg cttggccctg gtgaccggct
    10861 tcggcgtatt tttcctgatc gagaagctgg tcctgtggcg gcatgcgcat tcgcatgatg
    10921 gcgagggtgg cgggcacacc gcggaagctc atgcgggtga ggtgcacacc gcaggggcgc
    10981 atccgcattc cgggcatgga cactc
//
LOCUS       DASRHN010000390         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_310742, whole genome shotgun sequence.
ACCESSION   DASRHN010000390 DASRHN010000000
VERSION     DASRHN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310742"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..645
                     /locus_tag="VFB37_15770"
     CDS             157..645
                     /locus_tag="VFB37_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23957.1"
                     /translation="MGDNPGTEATGPSDTRGLDQEVQGLKKDVVDLNRELFVLEEELL
                     FPANTQVAVFVSMDVGDFFALDSITLKIDQKEVINYLYTPREVEALLKGGVHRLYLGN
                     LKVGKHELVAFFSGKGPNDRDYKRGATLKFEKGIGAKYLELKINDRQRRLQPEFEIKD
                     WE"
     gene            659..>2587
                     /locus_tag="VFB37_15775"
     CDS             659..>2587
                     /locus_tag="VFB37_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO23958.1"
                     /translation="MRAFALFIASLVVALAAASGPAVARRHDPEKLPVTRIRDLHYGD
                     VLFYFYQDDDFEAITRLTAYQHWNLIPHHEDEGELLLGGLYLSLGMHNEAGERFQTLL
                     TRDVPTGVRNRAWFYLAQVWYARGYLDKAEEALDRVQGKLSPELEAQKEHLFANVLMH
                     QGKFDQAIRVLVAWKGSPSWSAYARFNLGVALIREKRLTDAEAFLTAVGTMYAESGEM
                     LALKDRANLALGFAELQVDQPAKAKESLQRVRLNGPYSNKALLGMGWADAALGDYQAA
                     LNPWMELRGRNLLDAAVQESFLAVPYAFSKLNASAQSAEYYETAVESYDAETGRLDDA
                     ISRIQGGSLLEQVLSSEKESRYGWFWQLKSVPEAPESRYLYTVLAGHDFQEGLKNYRD
                     LAYMGHTLDKWSDSMEAFGDMIDTRERAYAERLPRVDALLASGAIDRLAQRRSELDSR
                     INTIEASKDVAAVGSPEEREQWGRIQRLEAALAGAPDNPQNAELRQRLRLVKGVLYFR
                     LDEAFKARMWQQHRSMKDLDLALQEARSRWIRVDRARRSVPTNNGEFATRVAALKTRI
                     DALQVRLAATEQKQSEYLAQVAVGELEQQKSRLATYQVQARFALATMYDRAANADQAQ
                     RAEAPEPAQASPAQSPVPS"
BASE COUNT          499 a          809 c          848 g          431 t
ORIGIN      
        1 cagggcgcag ccgccacggc gcctgcgcag acgagcccgc ctgcatccgc ccccgcgtca
       61 acggctgccg cccccgcaga ggggaacgag gccgcacccc cgccaggaac tgaacccgca
      121 acaccggcgc cggggactga agccgcccca cctgccgtgg gcgataaccc cggcacagag
      181 gcgaccggcc cgtcggacac ccgcgggctc gaccaggaag tgcagggact gaagaaggac
      241 gtcgtcgatc tgaaccgcga gctgtttgtt ctggaggaag agctgctctt cccggcaaat
      301 actcaagtcg ctgtgttcgt ctccatggac gttggggatt tcttcgcgct cgattcaatc
      361 accctgaaaa tcgatcagaa ggaagtgatc aactacctct acacgccacg cgaggtggag
      421 gctcttctca aagggggtgt gcaccgcctc tacctcggca acctgaaagt aggcaagcac
      481 gagctggtcg ccttcttcag cggcaagggt ccgaatgacc gcgactacaa gcgcggcgcg
      541 actctaaagt tcgaaaaggg catcggcgcc aagtatctcg agctgaagat caatgaccgg
      601 cagcggcgtc tgcagccgga gttcgagatc aaggactggg aataacgggt cgaagtccat
      661 gcgtgccttc gccctgttca ttgcttcgtt ggttgtcgcc ctcgccgcgg cgagcggtcc
      721 ggctgttgcc agacggcacg accccgagaa gctgcctgtt acccgtatcc gtgacctgca
      781 ttacggtgac gtgctctttt acttctacca ggacgatgac ttcgaggcga tcacgcgcct
      841 gacggcgtat cagcactgga atctcattcc tcatcatgag gacgaggggg agctcctgct
      901 cgggggcctc tacctgtccc tcggcatgca caacgaagct ggcgagcgct tccagacgct
      961 tttgacccgt gacgtgccga caggcgtgcg taaccgcgcc tggttctatc tcgcgcaggt
     1021 ctggtatgcg cgagggtatc tggacaaagc agaggaagcc ctggatcggg tccagggaaa
     1081 gctctccccg gagctggagg cacagaagga gcatctgttt gccaatgtcc tgatgcacca
     1141 gggcaagttc gatcaggcca tccgggtact cgttgcgtgg aagggctccc ccagctggtc
     1201 cgcctatgcg cgcttcaacc tgggtgtcgc gctcattcgt gagaaacgcc ttaccgatgc
     1261 ggaggcgttt ctgaccgccg tgggcacgat gtatgcggaa tccggcgaaa tgcttgcgct
     1321 caaggaccgc gcgaatctgg cgctgggctt cgcggaactg caggtggatc agcccgccaa
     1381 agcaaaggag tctctgcagc gcgttcggct caacgggccc tattcgaaca aggcgctcct
     1441 gggaatgggt tgggcggatg ccgcgctcgg tgactaccag gcggctctca atccctggat
     1501 ggagctgcgg gggcgcaatc tgttggatgc agcagtgcag gagtcttttc tcgccgtgcc
     1561 gtacgcgttc agcaagctca atgccagcgc acagtcggcc gaatactacg agacggcagt
     1621 ggagtcgtac gatgcagaga cggggcgtct ggacgatgcc atctcccgga ttcagggcgg
     1681 gagcctgctg gagcaggtcc tctccagcga aaaagagtcg cgctatggct ggttctggca
     1741 gctgaagagc gttccggagg cgcccgagtc ccgctatctg tataccgtcc tggccggtca
     1801 cgacttccag gaagggctga agaactaccg cgacctggcc tacatggggc acacgctcga
     1861 caaatggagc gacagcatgg aggccttcgg ggacatgatc gacacgcgcg agcgtgctta
     1921 cgccgagcgt ctgccacgag tcgatgcact gctggcctcc ggcgccatcg atcggctggc
     1981 gcagcgccgc agcgagctgg acagccgcat caacaccatc gaggccagca aggacgtggc
     2041 ggccgtcggc tcgccggagg agcgcgagca atgggggcgc atccagcgcc tggaggccgc
     2101 gctcgcgggc gcccctgaca atccgcagaa cgcggagctc aggcagaggc tgcgcctggt
     2161 gaagggggtg ctttacttcc ggctggacga ggcctttaag gcgcgcatgt ggcagcagca
     2221 ccgcagcatg aaggacctcg atctcgctct gcaggaggcg cgcagccgct ggatacgcgt
     2281 cgatcgggcc cgcagaagcg ttccgaccaa caacggagag tttgcaacac gcgtcgccgc
     2341 gctgaaaacg cgcatcgatg cactgcaggt gcgcctggcc gcgaccgagc agaagcagag
     2401 cgagtacctc gcccaggtcg cggtgggtga gctcgagcag cagaagagcc gcctggccac
     2461 ctatcaggtg caggctcgct tcgccctggc gacgatgtat gaccgggcgg cgaacgccga
     2521 ccaggcgcag cgcgccgagg ctccggaacc tgcccaggcc tcgccggctc agtcgccggt
     2581 gccatcg
//
LOCUS       DASRHN010000391        14077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2288598, whole genome shotgun sequence.
ACCESSION   DASRHN010000391 DASRHN010000000
VERSION     DASRHN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288598"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VFB37_15780"
     CDS             <1..1221
                     /locus_tag="VFB37_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO23959.1"
                     /translation="NPFAPPFVFRPLNVVLDQNTYTHKVTQEFRLSSPDKGPLEWLLG
                     AYYTRENSKIFPQQLVLLNAATDTVASDLPNFDSDLLASDLQEYAGFANATWHLVPRF
                     DLTFGGRYSHNKQDAVVDQDGTLNALVGSPPQIVASSAEHVFTYSVAPRLQITDTASV
                     YARIATGYRPGGPNVIPPGAPPGTPAFYKADRTTNYEAGLKSSWLDHRLSFDVSAFYI
                     AWKDIQLFAEINQVGVNTNGGTAFSRGVEFTVEALPVTGLTLSLNGAYTNAKLTEDTP
                     PATGGHEGDPLPYIPDWSIDLRAEYEWHWVDDFTPYVGAGINYVGKQVADFTERFASS
                     GDLMSLPTYEEYDVHAGLRRDHWSIEFYGKNLSNAQGRTEITGVIGASYPNNALAAAL
                     IRPRTVGVTLGWKF"
     gene            1238..3250
                     /locus_tag="VFB37_15785"
     CDS             1238..3250
                     /locus_tag="VFB37_15785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZO23960.1"
                     /translation="MIRQCLTATFAFVTSVAALAAAPTHKNPVPTGPAPAAAVAPAAN
                     PPETTPSATVPGGTHELTEADLATFLDGNVPYAMARGSIAGGVVVVVKDGKTLYAKGY
                     GYADVAGHKPVIADQTLFRPGSVSKLFTWTSVMQLVGAGKLDLDRDVNDYIDFKIPAK
                     FGKPITVRNLMTHTPGFEDGLSEAFVNKPEQLMPLGDYLKKHIPARIYPPGKIVAYSN
                     YGASLAGYIVQRLSGEQFADYVAHHIFKPLGMERSTFVQPLPAQFQSNMSNGYITATD
                     EKPHPFELIETAPAGALSATGTDMAHFMIAHLQNGRYGEERILPEQTALEMHSPVSRM
                     APGMNGFDLGFYQENRNGQRIIGHAGDTDPFHSDLHLLLDRNVGIFMSFNSLGKEGEA
                     GKVREELFRAFLDRYYPFSPPEEKTVADPKTDAARVAGWYVGSRRIESALRLLFGLSQ
                     TSIKAHEDGTIETSALKDLSGTPKRWREVGPLTYREVNGQTHLKFVTDEKGKIYYWIS
                     DDFLPVEVFMKTHGFEQLGVLRVMLIAFLAVLVLTMAVWLGGWIARRHYRTTLVLNPK
                     QQRLRLASRIAVIVVLLMAAGWLGLVAVLESGNGELNGLLITLYLLGVLAILACLVVL
                     FEAGQRVLNGPGGWLVRCGELLLGLAGLYGLWVIFDYGLANFSLTY"
     gene            3225..3878
                     /locus_tag="VFB37_15790"
     CDS             3225..3878
                     /locus_tag="VFB37_15790"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZO23961.1"
                     /translation="MRTSVSRTERALLACLCVAGTHVPASAVPPADLDAYAARALHAF
                     GAPGMAVAIVEGSRTYTRCYGVRTMGAPEPVDEHTTFPIGSNTKAFTSTALAILVDRG
                     KLAWSDRVVERLPGFEMFDPYASHEMTLRDLLTHRSGLGLSEGDLMFTPTTNRSRADV
                     VHALRYLRPATSSAVRADRPLRSGSLAPRRFLRGGVQGSVVWDGEHQFFGRGFAHAV"
     gene            3808..4107
                     /locus_tag="VFB37_15795"
     CDS             3808..4107
                     /locus_tag="VFB37_15795"
                     /inference="COORDINATES: protein motif:HMM:NF023380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HZO23962.1"
                     /translation="MYRDPWYGTASISSSGGGLRMRFDDTPGMEGTLEHVQYDTFRTH
                     WTDRDIEDAYVTFDLDFAGAIESLKMRAVSPSADFSFDYQDLHFVPLKPQTSHDH"
     gene            4097..5110
                     /locus_tag="VFB37_15800"
     CDS             4097..5110
                     /locus_tag="VFB37_15800"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HZO23963.1"
                     /translation="MIIELPQPYLLFLGDVTEAAYAKTAFGLRDWAPQLCVGEFSLPG
                     VTVTTGLPRLSPSEARARGARALVIGIANRGGVIAPVWLPSLVQALEEGLDIVSGMHN
                     RLSQQPELEQTARRLGRRLIDVRQPPPDIQIASGDKRSGKRLLTVGTDCALGKKYTAL
                     ALARAFRDLGVAADFRATGQTGILISGSGIPMDAVVADFVAGAAEMLSPAAAADHWDV
                     VEGQGSLFHPSYSGVSLGLLHGTQPDVIVLCHELGRDRMMGLESFPTPELEEAIDLHL
                     RLARRTNPAVRCAGVSLNTARASDGEARAILKEHSERLGLPVADPIRGGSEFTRLVSA
                     CLA"
     gene            5200..6219
                     /locus_tag="VFB37_15805"
     CDS             5200..6219
                     /locus_tag="VFB37_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HZO23964.1"
                     /translation="MNPRTRLSLHVETEKWPLKRPFRITGHTMHDCEVVVVNLTQDGL
                     KGRGEAAGVYYLQDDVPAMVKRIESVRDSIEAGIDREMLQSLLPAGGARNAVDCALWD
                     LDAKRMGKPAWELADLDTPRPLLTAHTVGAESPTDMADWARSYTEAKFLKLKLTGEAI
                     DADRIRAVRAARKDVSLSVDANQGFTRSSLQALLPVLTQARVTLVEQPFPIGQDAQLD
                     GFKFPIPIGADESIQGLKDLPGLVGRFGVVNIKLDKCGGLTEGLAIAREARRLGLDVM
                     VGNMTGTSLAMAPGYLVGQLCQVVDLDGPLFLSSDRTPAATFEGGKITCPEALWGAPL
                     SKPSA"
     gene            complement(6317..6745)
                     /locus_tag="VFB37_15810"
     CDS             complement(6317..6745)
                     /locus_tag="VFB37_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23965.1"
                     /translation="MKLLDPLIRVFVLTQSLGVTWLREELIALEVGIPLTDACLGEFV
                     RDADAAACGGARRALRYVEEMRRQVAIRARFIQRWTGSDDSIASQDDQSLQGLVRIAR
                     KHALPRRWKLSDPVVTEHRRTCPSYWNWTSELDTEALPQS"
     gene            complement(7965..8040)
                     /locus_tag="VFB37_15815"
     tRNA            complement(7965..8040)
                     /locus_tag="VFB37_15815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8005..8007),aa:Lys,seq:ttt)
     gene            complement(8214..9035)
                     /gene="ybgF"
                     /locus_tag="VFB37_15820"
     CDS             complement(8214..9035)
                     /gene="ybgF"
                     /locus_tag="VFB37_15820"
                     /inference="COORDINATES: protein motif:HMM:NF024902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HZO23966.1"
                     /translation="MRMNQADLPGRSPVRLRSASVAIAVLLTLAGCASDRDEEDAMQS
                     KINELDTRVARIERVVSNQSLVELAQNLDAVQADVRKLRGRVDELENSQEALRKQQRD
                     MYSDLDKRLGGGQGAAGAGAGAAGPNSGPGAGQGPVAGSSVEQAVYSQAFDALKAGSY
                     SVAITGFKDFLSTYPQSPLSENAQYWLGEAYYVTRDYGGASEAFHTVLQKWPDSRKAP
                     DALLKLGFTQFELKRYGESRKTLEQVTQKYPDSDAARLATERLKRMPANPPPASH"
     gene            complement(9042..9593)
                     /gene="pal"
                     /locus_tag="VFB37_15825"
     CDS             complement(9042..9593)
                     /gene="pal"
                     /locus_tag="VFB37_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008027793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZO23967.1"
                     /translation="MQKVLMVLMLAVAVGSAGCAHKKPKAPPPEQQSSESNSGADSSG
                     ANAANAGSVGSDDEAAGPQAGLLAKRVIYFDFDSSEIKSEGTEIVAAHAKYLASHSSA
                     HVRLEGNTDDRGSREYNIGLGERRAQAVRRALLLQGATEAQLSTVSYGAERPAAVGND
                     EGAWAKNRRVEIVYLNPNGQPQR"
     gene            complement(9631..10959)
                     /gene="tolB"
                     /locus_tag="VFB37_15830"
     CDS             complement(9631..10959)
                     /gene="tolB"
                     /locus_tag="VFB37_15830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HZO23968.1"
                     /translation="MRLNRSFSGVPKRLALGLACAISLGAALSARAQLKIEVTSGVSD
                     PVPIAIVPFANAPNDGGLDIAQVVQHDLEGSGRFRALPRAQMPGTPTQPSMVNFADWK
                     AAGDDYVVIGRVTPMDGGNLTVDFDLLNTLTGQRVTGQRFVGTPGAMRNAAHRVSDVI
                     YEKILGIRGAFATRIAYVAVGGQPPTQHYQLIVADADGENQRLILESRFPLMSPSWSP
                     DGQWLAYVSFESKRSAVYVQLVRTGERRQVSARAGINGAPAWSPDGHKLALTLGGSNG
                     NPDLYVVDLATQNLIRITDDPAIDTEAAWAPDGKSLYFTSDRAGSPQIYRVAAQPGEH
                     PKRITFTGSYNARPRVSPDGSQLAMVTLDGGNYRIAVQDLSNGTVRVLSHGHLDESPS
                     FAPNGATLIYSEQDGGRGVLATVSVDGLTGLRLKSDQGTVREPVWGPFGQ"
     gene            complement(10977..11885)
                     /gene="tolA"
                     /locus_tag="VFB37_15835"
     CDS             complement(10977..11885)
                     /gene="tolA"
                     /locus_tag="VFB37_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HZO23969.1"
                     /translation="MAERARTERASDRWVSIAMSVMLHGALVGALAYGFWTYHKSQPP
                     TPTLAIQAEVVNSRSLSTALKQVTPQPPKPAPTPTPTPAPTPPPQPPPEEPTGPPEPT
                     PEELAQREQVLKDQEQKKQAEKQAAEQRKLEEQQKQEQQRQDEAKRQAQERAEAERKA
                     AAKKRADAQRLAEQKRQEEAQRQADEQNRVQAEADLKQDLATEERTTSARSSAALASW
                     QAQIAARIQRAWLRPPTAKAGIECVLDVTQVPGGQVVNVRVGSCNGDQAVRDSIQDAA
                     YRASPLPPPPDPSLFERNLEITFKPD"
     gene            complement(11906..12358)
                     /gene="tolR"
                     /locus_tag="VFB37_15840"
     CDS             complement(11906..12358)
                     /gene="tolR"
                     /locus_tag="VFB37_15840"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HZO23970.1"
                     /translation="MTQVSRGRRLMGEINVVPYIDVMLVLLIIFMITAPLLTQGVKVD
                     LPKAGAEPLDAEMLKNAIPLVLSVDREGRLYLNVGGNPQAPLNPDIVAARATAALRRN
                     PLLPVLVKADNRVEYGRVVQAMVILQQAGAQKVGFITDPLPTPPRRGH"
     gene            complement(12371..13075)
                     /gene="tolQ"
                     /locus_tag="VFB37_15845"
     CDS             complement(12371..13075)
                     /gene="tolQ"
                     /locus_tag="VFB37_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZO23971.1"
                     /translation="MSEQLSVIRIIAEASVPVQIVIGLLLLASLSSWAIIFRKRLVIG
                     RARRSADQFENTFWSGGDLAQLYRAIEQRGGAIGMASIFEFGFREFARLRQQGSIGSD
                     QLLEGARRAMRVAHLKEIDRLEASLATLATVGSTSPYVGLFGTVWGIMSAFSSLGNVQ
                     QATLAMVAPGISEALIATAIGLFAAIPAVVAYNRFADQVGRLEIRFDAFTEEFSTILQ
                     RHAARGNSATSAPTAA"
     gene            complement(13072..13530)
                     /gene="ybgC"
                     /locus_tag="VFB37_15850"
     CDS             complement(13072..13530)
                     /gene="ybgC"
                     /locus_tag="VFB37_15850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HZO23972.1"
                     /translation="MSVFAWPARVYWEDTDAGGIVYYANYLRFLERARTEWLRSLGHS
                     QRALVEEQGVVFTVVSLEADYRRPARLDDELMITCEPRVEGAASVRFGQRIFRGTGEP
                     DSERLLLQASVRVACLDAQTLKPKRLPEFLLRSTIERAPPRGRSSRSQVE"
     gene            complement(13527..>14077)
                     /locus_tag="VFB37_15855"
     CDS             complement(13527..>14077)
                     /locus_tag="VFB37_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HZO23973.1"
                     /translation="GLLTSPLRDRFGIVQRLEFYGVEDLEKIVGRSASILGVPLDEGG
                     GRRIAQRSRGTPRIANRLLRRVRDYAQVKADGAIDERIAEAALDLLEVDPLGFDTLDR
                     KLLLTIIERFDGGPVGIDSLAAAIGEERGTLEDVIEPYLIQQGFLVRTARGRVVTRAS
                     YLHFGLKPPERAANLQLFEDGR"
BASE COUNT         2476 a         4600 c         4430 g         2571 t
ORIGIN      
        1 aatccattcg ctccgccctt cgtcttcagg cccctgaacg tcgtcctcga ccagaacacc
       61 tacacccaca aggtgaccca ggagttccgc ctctcctcac ccgacaaggg gccgctcgag
      121 tggctgctgg gtgcttacta cactcgcgaa aattccaaga tcttcccgca gcagctcgtg
      181 ctcctcaatg ccgccaccga cacggtcgcc tccgaccttc caaacttcga tagcgacctg
      241 ctggcctccg acctgcagga atatgccggc tttgccaatg ccacctggca cctcgtgccc
      301 cggttcgacc tgaccttcgg cgggcggtat agccacaaca aacaggatgc cgttgtcgat
      361 caggacggaa ccctgaacgc gctggtggga tcgccgccgc agatcgtcgc ttcgtcggct
      421 gagcatgtgt tcacgtattc ggtcgcaccg cgcttgcaga tcaccgatac ggcctcggtg
      481 tatgcgcgca tcgccacggg ctatcgcccg ggcggcccga acgtgattcc gcctggcgca
      541 ccgcccggca ctcctgcctt ctacaaggcg gatcgcacga cgaactatga agcgggactg
      601 aagtccagct ggctcgacca tcgactctca ttcgacgtgt ccgcgttcta tatcgcctgg
      661 aaggacatcc agctttttgc ggaaatcaat caggtgggtg tgaacaccaa tggtggcacg
      721 gccttcagcc gcggcgtcga attcaccgtc gaagcactgc cggtgacggg cctgacgctg
      781 tccctcaacg gggcctatac caacgccaag ctcaccgagg acacccctcc tgccaccggc
      841 ggccacgagg gcgatccgct gccctacatt ccggactgga gcatcgatct gcgtgccgag
      901 tacgaatggc actgggtcga tgatttcacg ccttacgtgg gcgccggcat caactatgtg
      961 ggcaagcagg tcgccgattt cacggagcgg tttgcctcct cgggagacct gatgagtctg
     1021 cccacctacg aggaatacga tgtgcacgcc ggcctacggc gcgatcactg gtccatcgag
     1081 ttctatggca agaatctcag caacgcgcag ggccgcaccg agatcaccgg tgtcatcggt
     1141 gcgagctatc ctaacaatgc gctggctgca gctctcatcc ggccacgcac ggtgggcgtg
     1201 acactgggat ggaagttctg atcaaacgga gagtaccgtg atccgtcaat gcctgaccgc
     1261 gaccttcgcc ttcgtcacgt cggtcgccgc gctcgccgcc gctccgacac acaagaatcc
     1321 cgttccgacc gggcctgccc cggctgcggc cgtggcaccg gcggccaatc cgccggagac
     1381 aactccatca gcgaccgtgc ccgggggcac gcatgagctc accgaagcgg acctggcaac
     1441 gttcctcgat ggcaacgtgc cctacgccat ggcccgcggc agcatcgctg gtggggtggt
     1501 cgtggtcgtc aaggacggca agacgctcta tgccaagggt tacggttacg cggatgtcgc
     1561 cggccacaag cccgtgatcg cagatcagac gctgttccgc cccgggtcgg tctccaagct
     1621 cttcacctgg acctccgtca tgcagctggt cggcgccggc aagctcgacc tcgaccgcga
     1681 tgtgaacgat tacatcgatt tcaagattcc ggcgaagttc ggcaagccca tcacggtgcg
     1741 caacctgatg acgcacacgc ccggcttcga ggatggactg tccgaggcgt tcgtgaacaa
     1801 gcccgagcag ctcatgcctc tcggggatta tctgaagaag cacattccgg cacgcatcta
     1861 tccacccggc aagatcgtcg cctattccaa ctacggcgcc tcgctggccg gctacatcgt
     1921 gcagcgcctc tcgggtgagc agttcgccga ctatgtggcg caccacattt tcaagccgct
     1981 cggcatggag cgttcgacct tcgtgcagcc gttgcccgcg cagttccaat cgaacatgtc
     2041 gaacggctat atcaccgcca ccgacgagaa gccgcatccg ttcgagctga tcgagaccgc
     2101 gcccgccggc gcactcagcg cgacgggcac cgacatggct catttcatga ttgcgcacct
     2161 gcagaacggc cgctacggcg aggagcgcat cctgcccgag cagaccgctc tggagatgca
     2221 ttcgccggtc tcgagaatgg ctcccggcat gaacggattc gacctcggct tctatcagga
     2281 aaaccgcaac ggccagcgca tcatcggcca cgccggcgat accgatccgt tccacagcga
     2341 tctgcacctg ctgctggatc gcaacgtggg catcttcatg tccttcaaca gcctcggcaa
     2401 ggaaggcgag gcgggcaagg tgcgcgagga gctgttccgc gcattcctgg accgctacta
     2461 ccctttctcg ccgcccgagg agaagactgt cgccgatccg aagaccgatg cggcgcgcgt
     2521 ggccggctgg tatgtcggca gccggcggat cgagagcgcc ttgcggctgc tgttcgggct
     2581 cagccaaacg agcatcaagg cgcacgagga cggcaccatc gagaccagcg cactcaagga
     2641 tctgagcggc acaccgaagc gctggcggga agtgggtccc ctcacctacc gcgaggtcaa
     2701 cggccagacg catctgaaat tcgtcactga tgagaagggc aagatctatt actggatctc
     2761 cgatgatttc ctgcccgtcg aggtcttcat gaagacccac gggttcgagc aactcggggt
     2821 gctccgggtc atgttgatcg cattcctggc ggtcctcgtc ctgacgatgg cggtgtggct
     2881 gggcggctgg attgcccgac gtcactaccg cacaacgctc gtgttgaacc cgaaacagca
     2941 gcggcttcgc ctggcctcgc gtatcgcggt catcgtggtg ctgctgatgg cagcgggatg
     3001 gctcggcctg gtcgccgtgc tcgagagtgg caatggtgag ctcaacggcc tgctcatcac
     3061 gctttatctg ctgggtgtac tggccatcct ggcgtgtctg gtcgtgctct tcgaagccgg
     3121 ccagcgtgtc ctcaacggcc ccggcggatg gctcgtgcgc tgcggagagc tgctgcttgg
     3181 acttgcgggc ctctacgggc tgtgggtcat cttcgactat gggcttgcga acttcagtct
     3241 cacgtactga gcgcgcgctg ctcgcctgcc tgtgtgtcgc cggcacccat gtgccggcga
     3301 gcgcggtgcc gccggccgat ctggatgcgt atgccgcgcg ggccctgcat gcctttggcg
     3361 cccccggcat ggccgtggcg atcgtggagg gatcgcgaac ctacacgcgc tgctacgggg
     3421 tacgcaccat gggtgctccg gagccggtgg acgagcatac gacctttccg atcggctcga
     3481 ataccaaggc gttcacctca accgcactcg ccatcctggt cgatcgtggc aagctcgcct
     3541 ggagcgatcg agtggtcgag cgactgccgg gcttcgagat gttcgatccg tacgcctctc
     3601 acgagatgac actgcgggac ctgctgactc atcgcagcgg tctcggactc agcgagggtg
     3661 acctgatgtt cacgcccacg accaaccgct cgcgggcgga tgtcgtgcat gccttacgct
     3721 atctgcggcc ggcgaccagc agcgcagtcc gagcagaccg cccgctccga tcggggtcgc
     3781 tcgctccccg tcgcttccta cgtgggggtg tacagggatc cgtggtatgg gacggcgagc
     3841 atcagttctt cgggcggggg tttgcgcatg cggtttgatg acacgcctgg aatggaaggc
     3901 acgctcgaac acgtacaata cgacacgttc cgcactcatt ggaccgatcg cgacatcgaa
     3961 gacgcctatg ttaccttcga tctcgatttc gcaggcgcca tcgagagcct gaagatgcgg
     4021 gccgtgtcgc ccagcgccga tttcagcttc gattaccagg atctgcactt cgttccgcta
     4081 aagcctcaga cttcccatga tcattgagct cccgcagcct tatctgttgt tcctcggcga
     4141 tgtgacggag gctgcctatg cgaagaccgc cttcggcctg cgcgactggg cgccgcagct
     4201 gtgcgtcgga gaattcagct tgcccggagt cacggttact acgggactgc cacggctgtc
     4261 gccgtcggag gccagggctc gaggcgcgcg cgcactggtg atcggcatcg ccaatcgagg
     4321 cggcgtgatc gcacccgtct ggcttccttc gctcgtgcag gcgctcgaag agggtctgga
     4381 tatcgtgagc ggcatgcaca atcggctctc gcagcaaccc gagctcgagc agaccgcgcg
     4441 ccgcctcgga cgacggctca ttgacgtacg ccagccccca ccggacattc agatcgcgag
     4501 cggcgacaag cgaagcggta agcgcttgct cacggtgggt accgactgcg cactgggcaa
     4561 gaagtacacg gcgctcgcgc tggcgcgcgc gttcagggat ctcggcgtgg cggccgattt
     4621 ccgggcgacc ggacagacgg gcatcctcat ctccgggagc ggcattccca tggatgcggt
     4681 cgtggcagac ttcgtcgccg gagccgccga gatgctcagc cccgcagccg ccgccgatca
     4741 ctgggacgtg gtggaaggtc agggctcatt gttccacccc tcctattccg gggtctccct
     4801 cggattgctg catggcactc agcctgatgt gatcgtgctc tgccacgaat tgggccggga
     4861 tcggatgatg ggtctggagt cgttcccgac accggaactc gaggaagcca tcgacctgca
     4921 cctacgcctg gcccggcgga ccaaccctgc cgtgcgctgt gccggcgtgt ccctgaacac
     4981 ggctcgggcc tccgacggtg aagctcgcgc catcctcaaa gagcacagcg agcgcctggg
     5041 ccttcctgtc gccgacccga tccgcggcgg cagtgagttc acccgactcg tgtcggcctg
     5101 cctggcgtag actgagggag cctcgccgct cgcgcgcggg taaaccccca gacgctgtca
     5161 tcctgcccag acctgaagtc aatcaaggac tcgaagctca tgaatccgcg cacgcgcctg
     5221 tcactgcacg ttgaaaccga gaagtggcca ctgaagagac ctttccgcat caccggccac
     5281 acgatgcacg attgcgaagt ggtggtggtg aatctcactc aggacggtct caaggggcgc
     5341 ggtgaggccg ctggagtcta ttacctccag gacgatgtgc cggcgatggt gaagcgcatc
     5401 gagagcgtgc gggactcgat cgaagcaggg atcgaccgcg agatgctgca gagcctgctg
     5461 cccgcgggtg gggcccgcaa tgccgtggat tgtgcgctgt gggatctcga tgccaaaagg
     5521 atggggaaac ccgcttggga gctcgccgat ctcgacacgc cccgcccgct tctcactgca
     5581 catacggtcg gtgccgagag ccccacggac atggcggact gggcacgctc gtacacagag
     5641 gccaagttcc tcaagttgaa gttgacgggc gaggccatcg atgcggatcg gatccgggcg
     5701 gtacgcgccg cgcgcaagga cgtctcgcta tcagtcgatg cgaatcaggg attcacgcgc
     5761 agctcactgc aggcactcct tccggtgctc acgcaggcac gcgtgaccct cgtcgagcag
     5821 ccctttccca tcgggcagga cgcgcagctc gatggtttca agtttccgat cccgatcggc
     5881 gccgatgaga gcatccaggg cctgaaggac ctgccggggc tggtgggacg cttcggtgtc
     5941 gtcaacatca aactcgataa atgtggaggc ctcaccgagg ggctggcgat cgctcgggag
     6001 gcgcgccgtc tgggactcga tgtgatggtc ggcaacatga ccggcacctc gctcgccatg
     6061 gcgcccggat acctggtggg tcagctgtgc caggtggtgg atctcgatgg cccattgttt
     6121 ctgagctcgg accgcactcc ggcggcaacc ttcgagggcg gcaagatcac ctgccccgaa
     6181 gctttgtggg gagccccttt gagcaagcca tccgcatagc ggactggtcg gcgcaatgtg
     6241 acccgcgcat gcagcgaggc cggagtggcc aaatccctgc gtccactccg gcctcattta
     6301 cggctgaatg gattactcag gactgcggca gggcctccgt gtcgagctcg ctcgtccagt
     6361 tccaatacga ggggcacgtg cgccgatgct cggtcacgac cggatcagag agtttccaac
     6421 ggcgtggcag tgcgtgcttg cgcgcaattc ttacaagtcc ctgcagactt tggtcgtctt
     6481 gggatgcaat ggaatcatca gatcctgtcc aacgttgaat gaacctggct cggatcgcca
     6541 cctggcggcg catctcttca acataacgta gtgcgcgcct cgcgcctccg catgcagcag
     6601 cgtccgcatc gcgcacgaac tcgccaaggc atgcgtccgt aagcgggatg ccgacctcga
     6661 gcgcaatgag ctcctcccgg agccatgtca ctccaaggct ctgggtaaga acgaatactc
     6721 gaatcaacgg atcgagcagt ttcatgtgta ttcccctttt gtcccggtgt ggggatccga
     6781 tcggcgtggg ctctggtcaa agctgagcga atgtcgtacg gacgccacac ggccgatatc
     6841 ccgtgttcta ttttgcacgc atcgtgccaa gcattggccc gcacacgaca atgtgtttca
     6901 gcgcccatgc ggattgaact caatccgcat gtccgcagcg ccgagccgtg tgcatggggc
     6961 aacactcgcc agagcttttg gtgcagcagg cgcgcaaacc gacgcacgac tcgagcggag
     7021 tccggctctt taccgttgtg acttttgaca gatgagccgg ttggcccagc cgcagcaaac
     7081 tgcgcgtatg aacatgcggt cctttctcaa gcttcgggcg cgcgcactcc accgcgtcaa
     7141 atgggccggg ctgacgctga cgatcgtgct ggcttatgta catccggcca ccgaggatcg
     7201 cgtgctcaag ggttggatct ggacgatcgc actcgcgacg ggtgttgggg tgttgtggct
     7261 tcaaatgcga tcgcggtgcc cgcggtgccg caacagggta cggctggatc ccgtagcagc
     7321 gtgctgtcct cgctgtgggc tcgactttgg cagctgcgca gctcgctcaa tgacccgagc
     7381 gcgcagcgcg tccggcacga gcagcccagc gcgcgaaaca tcttcaggac gaggagtgta
     7441 gaagtcttta gaactgtcag actcagccga gctaagaaat caataaatcg gatcgatcgc
     7501 gcggctcctg gtccacaatc gcttcatgcg ccgaccctgc ccgggggcga gggggggtgc
     7561 ggggcaactc gaattgctac cgacggagaa gcgtgtagcg atctgtgatg aaggcgagct
     7621 cttcaaactt cacggcccga ccgaacgcga gtcttgagag cccgctcgcc aagcgctgct
     7681 cttccgcacc tgccacctgc gatggttcac cgcctataaa aagggatggc gggaccggag
     7741 cgcgctcgag ccggaggctc atcgtatggg gggcgatggt gctcatcaga actgcggcgg
     7801 ttctggcggt gctggtggcc gtgcaggcgt tcttccgctg gatggtgcag ggcttcggga
     7861 tgcccgcact ctgagcggct gactccgtgt tcgccgcaat tctcagccaa cgaatcggag
     7921 ggcgcgacga gccaatatgc gcgggctggg ccggcatcgg gatttggtgg gccgtgcgcg
     7981 gatcgaacgc gcgaccaaca gattaaaagt ctgctgctct accgactgag ctaacggccc
     8041 attgtggagg gaatcccgag cgccgagcgc acatgccgcc ggcgggggcc gggcatcata
     8101 ctacaggagg ccgctagaga ctcacggggc tacgcgggac cgcggcgggc ggggtggcca
     8161 cgcgaacgcg caagggttgg ctacggtcgc gcgcgaccgt gcggcccgca agctcagtgc
     8221 gaagcaggcg gcgggttggc gggcatgcgt ttcagacgct ccgtagccag acgggcggca
     8281 tcgctgtcgg gatacttctg ggtcacctgt tccagcgtct tgcgcgactc cccgtaacgc
     8341 ttcagctcga attgggtgaa cccgagcttc agcagcgcat ccggggcttt gcgcgaatcc
     8401 ggccacttct gcagcaccgt atggaatgcc tccgaggcac cgccatagtc gcgggtcacg
     8461 taataggcct cgcccagcca gtactgtgcg ttctccgaga gggggctttg tgggtaggtc
     8521 gacaggaagt ccttgaatcc cgtgatcgcc accgagtagc tgcccgcctt gagcgcatcg
     8581 aaggcctgcg aatacaccgc ctgctcgacc gaggagcctg cgaccggtcc ctggccggca
     8641 ccgggccccg agttggggcc cgcagctccc gcccccgcac cggctgcacc ctggccgccg
     8701 cccaggcgct tgtcgagatc gctgtacatg tcgcgctgtt gtttacgcag cgcttcctgc
     8761 gagttctcga gctcatcgac ccggccccgc agcttgcgca catcggcctg cacagcatcc
     8821 agattctgcg ccagctccac caggctctgg ttcgagacca cccgctcgat ccgcgcgacg
     8881 cgggtatcga gctcgttgat cttgctctgc atcgcatcct cctcgtcgcg atccgacgcg
     8941 cagccggcga gcgtcaggag cactgcgata gccacggagg cggagcgaag tctcaccggg
     9001 gagcggcccg gcagatcggc ctggttcatg cgcatggagg attacctctg cggctggcca
     9061 ttcggattca gatagacgat ctcgacacgg cgattcttgg cccaggcccc ttcgtcgttg
     9121 cccaccgcgg ccggccgctc cgcgccatag ctcaccgtgg acagctgggc ttcggttgca
     9181 ccctgcagca gcagcgctcg acgcacggcc tgtgcgcgcc tttcgcccag accgatgttg
     9241 tattcgcgtg agccgcgatc gtccgtgttg ccctccagac gcacatgggc gctggaatgg
     9301 ctggccagat acttggcgtg ggcggcgacg atctcggttc cttcgctctt gatctcgctg
     9361 ctgtcaaagt cgaagtagat gacccgtttg gcgagcaacc cggcctgcgg gccggcggcc
     9421 tcgtcatcgg aaccgaccga gcccgcattc gcggcattcg cccccgagga gtccgccccc
     9481 gagttcgact cggaagactg ctgttccggt ggcggcgcct tgggcttctt gtgcgcgcat
     9541 ccggcgctcc ccacagcgac cgccagcatc aggaccatca gtactttttg cataccactc
     9601 cacccttgcg ccgcgattgc ggcaatcatt ttactgaccg aacggacccc agacgggctc
     9661 gcggactgtt ccctgatccg acttcagtct cagtcccgtc aaaccatcca ccgacaccgt
     9721 ggcgagcacc ccgcgaccgc catcctgctc ggagtagatc agcgtcgcgc cgttgggcgc
     9781 gaagctgggc gactcatcca gatgcccgtg cgagagcacc cggaccgtac cattcgaaag
     9841 atcctgcacc gcgatgcgat agttgccacc gtcgagcgtc accatcgcaa gctggctccc
     9901 atcaggggac acccgcggcc gtgcattgta agaccccgtg aaggtgatgc gcttcggatg
     9961 ctcgcccggc tgagcggcca cccgatagat ctgcggactg ccggcacggt ccgaggtgaa
    10021 atacagcgac ttcccgtccg gggcccacgc cgcttccgtg tcgatcgcgg gatcgtccgt
    10081 gatgcggatc agattctgcg tggcgagatc gacaacgtac agatctggat tgccgttgct
    10141 gccgccaaga gtcaacgcca gcttgtgccc atcgggagac caggccggcg ctccattgat
    10201 gccggcgcga gcggacacct ggcggcgctc cccggtgcgc acgagttgga cgtacacggc
    10261 ggagcgcttg ctctcgaacg acacgtaggc aagccactgg ccatccggcg accacgaagg
    10321 ggacatgagc ggaaaacgcg attccagtat gagccgctgg ttctcaccat cggcatcggc
    10381 cacgatcagc tgataatgct gcgtcggagg ctggccgccc accgccacgt acgcgatgcg
    10441 tgtggcgaag gctccgcgaa tgcccaggat cttttcgtag atgacatcgc tgacgcgatg
    10501 ggctgcatta cgcatggccc ccggcgtccc gacgaatcgc tgtcccgtga cacgctgacc
    10561 ggtgagcgta ttcagcagat cgaagtccac ggtgagatta ccgccgtcca tcggggtgac
    10621 acgtccaatg acgacgtagt catcgcctgc agctttccag tcggcgaaat tcaccatgga
    10681 tggctgtgtc ggggtgcccg gcatctgggc ccgcggcagt gcgcggaagc ggccgctgcc
    10741 ctccagatcg tgttgcacga cctgggcgat atccagcccg ccatcgttgg gagcattcgc
    10801 gaacggcacg atcgcgatcg gcaccggatc gctgacgccc gaggtgacct cgatcttgag
    10861 ctgtgcccgc gcagagaggg ccgcgcccag gctgatcgcg catgccagac cgagggcgag
    10921 ccgcttgggc acaccggaaa agcttctgtt gagtctcatg ctgttgaagt ttgacatcaa
    10981 tcgggcttga aggtgatctc aagattacgc tcgaacagcg acgggtcggg cggaggcggt
    11041 aacggagatg cgcggtacgc ggcatcctga atcgagtcgc gcacggcctg atcgccattg
    11101 cagctgccta ctcgcacgtt gacgacctgg cctccgggga cctgcgtcac gtccaacacg
    11161 cattcgatgc ctgcttttgc agtaggcggt cgcagccacg cccgctgaat gcgcgcggcg
    11221 atctgcgctt gccagctcgc aagcgccgcg ctggagcgag ccgatgtagt gcgctcctcg
    11281 gtagcgagat cctgcttcag gtcggcctct gcttgcacgc gattctgctc atcggcctgc
    11341 cgttgtgcct cctcctggcg cttctgctcg gccagacgct gggcatccgc tcgctttttt
    11401 gcggccgcct tgcgctcagc ctccgctcgc tcctgagcct gccgcttcgc ctcatcctgg
    11461 cgctgctgct cctgcttctg ctgctcctcg agtttgcgct gctcagcggc ctgcttctcg
    11521 gcctgctttt tctgctcctg atccttcagc acctgctcgc gttgcgcgag ctcctccgga
    11581 gtcggctccg gcggtccagt cggctcttca ggcggcggtt gcggaggcgg agtcggggcg
    11641 ggcgtggggg tcggggtcgg ggctggcttg ggcggttggg gggtgacctg cttcagggcg
    11701 gtgctcaggc tgcgtgaatt cactacctct gcctggatcg ccagcgttgg tgtcggaggc
    11761 tgcgacttgt gatacgtcca gaagccgtaa gccagagctc ccacgagcgc gccgtgcagc
    11821 atgacggaca tcgcgatgga aacccagcga tcgctcgcac gctccgtcct tgcacgctcg
    11881 gccatggagg ctcgcgcgct ccctgtcagt ggccgcggcg cgggggagtc ggcagcggat
    11941 cggtgatgaa accgaccttc tgagccccgg cctgctgcag gatcaccata gcctgcacca
    12001 cgcggccgta ctcgacgcgg ttgtccgctt tcacgaggac gggcagaagc gggttgcgtc
    12061 gcaaagccgc cgtcgcccgc gccgctacga tgtccgggtt gagcggggct tgcggattgc
    12121 cgccaacgtt cagatatagc ctgccctcgc gatccaccga caggaccagt gggatggcat
    12181 ttttgagcat ttccgcatcg agaggttccg cgccggcctt cggcaggtct accttgaccc
    12241 cctgggtgag cagcggcgcc gtgatcatga aaatgatcaa cagcaccagc attacatcga
    12301 tatagggcac cacgttaatc tcacccatga gccggcgtcc gcgagagact tgcgtcatgc
    12361 tggcgagctc ctaagccgcg gtgggagcgg atgtggcact gttgccgcgg gcggcgtgtc
    12421 gctgcaggat cgtggaaaac tcctctgtga aggcatcgaa acgaatctcc agccgcccca
    12481 cctgatcggc gaagcggttg taggcgacca ctgcgggaat cgccgcaaac agcccgatgg
    12541 ccgttgcgat gagcgcctcg gagatcccgg gcgccaccat cgcaagggtg gcctgctgga
    12601 cattgccgag cgaggagaac gcactcatga tgccccacac ggtgccgaac aggcccacgt
    12661 aagggctcgt cgagcccact gtcgcgaggg tcgcaagact tgcctccagg cggtcgatct
    12721 ccttcaaatg cgcgacgcgc attgcccgcc gggccccctc cagcagctgg tcggagccaa
    12781 tcgaaccctg ctggcgcagc cgcgcaaatt cccggaatcc gaactcgaag atgctcgcca
    12841 taccgatggc accgccgcgc tgctcgatcg cgcggtacag ctgtgccaga tcaccccccg
    12901 accagaaggt gttctcgaac tgatcggcgc tgcggcgcgc gcggccgatc acgagacgtt
    12961 tgcggaagat gatcgcccag gaggacagcg aggccagcag cagcaggcca atgacgatct
    13021 gcaccggcac actcgcctcg gcgatgatgc ggattacgga gagctgctca ctcattcaac
    13081 ctgactcctt gacgaacggc ctcgcggcgg agcgcgctcg atcgtcgacc gcaaaagaaa
    13141 ctccggcagc cgcttcggct tcagcgtctg cgcatcgagg caggcgaccc gaacgctcgc
    13201 ctgcaggagc agccgctcgg aatccggctc gccggtgccg cgaaagatgc gctggccaaa
    13261 gcggacactc gccgccccct cgacccgcgg ctcgcaggtg atcatcaact cgtcatcgag
    13321 ccgagccggg cggcgatagt cggcctcgag gctgaccacg gtgaacacca cgccctgctc
    13381 ctcgaccagg gcgcgctgcg agtgccccag cgagcgcagc cactcggtcc gcgcacgttc
    13441 gaggaatcgc aggtaattgg catagtagac gatcccgcct gcgtccgtgt cctcccaata
    13501 cactcttgcc ggccatgcga atacgctcat cggccatcct cgaagagctg caggttggcc
    13561 gcccgctcgg gtggtttgag gccgaagtgc agataggaag cgcgtgtcac tacacggccg
    13621 cgcgccgtgc gcaccaggaa accctgttgg atcaggtagg gctcgatgac atcctcgagc
    13681 gtaccgcgct cctcgccgat cgcagccgcc agactgtcga ttccgaccgg accgccatcg
    13741 aagcgctcga tgatggtgag cagcagcttg cgatcgagag tgtcgaatcc aaggggatcg
    13801 acttcaagca ggtcgagagc tgcctcggca atgcgctcat cgatggcgcc atcggctttc
    13861 acctgcgcgt aatcccggac ccggcgcagc aggcgattgg cgatgcgcgg cgtaccgcgt
    13921 gagcgctggg cgatgcggcg gcctcctcct tcatcgagcg gcacgccgag aatggaggcc
    13981 gagcgcccga cgattttctc caggtcctcg acaccataga actcgagccg ctggacgatc
    14041 ccgaatcgat cccgcaaagg cgaggtcagc agacccg
//
LOCUS       DASRHN010000392         6710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1959555, whole genome shotgun sequence.
ACCESSION   DASRHN010000392 DASRHN010000000
VERSION     DASRHN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959555"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFB37_15860"
     CDS             complement(<1..609)
                     /locus_tag="VFB37_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO23974.1"
                     /translation="MIEAKHLRKSFGSVTAVRDVSLRALDGRITGLLGPNGAGKSTTL
                     RILYTVIAPESGDALIDGVSAVTQPLEARRRMGVLPHNAGIYPHLSARENIAYYGALH
                     DLSRGERDRRTAELLGLLEMEDFADRPARGLSQGQRIKTALARALIHRPRNVLLDEPT
                     NGLDVIAVRTLRRLLGRLRDEGHCVLFSSHVMQEVGALCDEVI"
     gene            complement(606..2078)
                     /locus_tag="VFB37_15865"
     CDS             complement(606..2078)
                     /locus_tag="VFB37_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO23975.1"
                     /translation="MTHPTVSLLLALGLLAAADGAQASASLVLSPCELQDPLQVHSVT
                     AECGTLSVPENPQDPQGRHISLHVARVSAISRRKRPDPLFVFAGGPGMAATAFYASAS
                     FAFDWIHRERDIVLVDQRGTGDSNPLTCALDDTELYRASDAQIAAQTQLCLASLKPKA
                     RVEFYTTSIAVKDLDAVRGALGYESINLYGVSYGTRIAQHYLRRYPDRVRSVILDGVV
                     PPQIALGPAMAGNAEAALRSILARCASDAGCHAQFGDPLLSYQSLHSSLQARSVPVDL
                     FDPTSGQRVRFEFSPYHFAAVLRLVTYTSEQAALLPLILHRAAQSGDFTPLAAQFLLV
                     TREVRDAIAYGMHNSIVCSEDVPFYDLAAVNISEMQKTFLGTTQLEGLRNICRIWPRG
                     PIDPDFHSALHTDRPVLLLSGGADPVTPPRDAELARQGFTHSLHVVLKGFGHGQLTAP
                     CVGKLMASFLDAGGTAGVDASCTQADRPMPFFTSLGGPSP"
     gene            2188..4098
                     /gene="mnmG"
                     /locus_tag="VFB37_15870"
     CDS             2188..4098
                     /gene="mnmG"
                     /locus_tag="VFB37_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZO23976.1"
                     /translation="MRFAHKFDVIVVGGGHAGTEAALAAARMGARTLLLTQSIETLGQ
                     MSCNPAIGGIGKGHLVKEIDALGGLMARAADRAGIQFRTLNSSKGPAVRATRAQADRQ
                     LYRQAVRSALENQPGLALFQQEAADLLVEGGRVGGVVTVTGITFEARAVVLTVGTFLG
                     GRIHVGLDSYPGGRAGDQPSNRLAARMRELPLRTGRLKTGTPPRIDGRTIDPTVMQRQ
                     PGDTPVPVFSFLGRGSEHPEQLDCFITSTNERTHAIIRAATDRSPLFTGRIEGVGPRY
                     CPSIEDKIIRFADKPSHQIFVEPEGLNTVELYPNGISTSLPFDVQYELVRSIRGFENA
                     HLTRPGYAIEYDFFDPRDLEPSLQTRVLAGLFFAGQINGTTGYEEAAAQGLVAGINAC
                     LISREREAWIPARSEAYLGVLIDDLVTCGTREPYRMFTSRAEHRLLLREDNADLRLTP
                     TGRSLGLVDDERWALFEAKQALSDQEAERLQAIRVRPASVPADWANRVLRSPLTREGS
                     AFELLRRPEVGYVDLIEIAGAPDWTGADDRLPVQVRAQLEVRAKYAGYIERQQDEIER
                     QRRNEETRLPADLDYLDVPGLSHEVRQKLSEIRPATVGQASRIPGVTPAAISILLVHL
                     KKRGLAERARVA"
     gene            4095..4925
                     /gene="pyrF"
                     /locus_tag="VFB37_15875"
     CDS             4095..4925
                     /gene="pyrF"
                     /locus_tag="VFB37_15875"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZO23977.1"
                     /translation="MSPFLQQLAASWERSDSLICVGLDPEIERFPPHVAAQPSPIFQF
                     NRAIIDATADLVCAYKPQFAHYAAYEAEDQLERTIEYIHRSYPGVPVILDSKRGDVGN
                     TAERYAIEAFERYGADAVTVNPYLGADSVEPFLKYTDKGVIVLCRTSNPSSRDLQDLE
                     VGGRKLYQVVAEMVCRKWNSRGNCLLVVGATYPRELAEVREIAGSMPFLVPGVGAQGA
                     DVAQAVLNAQTPDGTGLIISSSRGILYRSLGDDFAAAARSAAEKLRNQVNESRSRRAR
                     "
     gene            4915..6600
                     /locus_tag="VFB37_15880"
     CDS             4915..6600
                     /locus_tag="VFB37_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HZO23978.1"
                     /translation="MRAEAARAFSRPMHVLCANRLALIAALIFALSVLTVAGCSRNPV
                     SGSANGSGAAILLRGGGPEPDSLDPQKARAVESQGILRDLCEGLTTLAKDASTAPGVS
                     DQWTVSADGRSYTFKLRPNARWSNGDRVVAADFVAALRRLVDPATASPYAQVIDIITN
                     ASDIVAGKKPPESLGVTAPDESTVSIQLAAPATYLPGLMAHPSTCPVHRGTLAQHPSE
                     LARPGVMVSNGAFYLKEWVQGSHILLLRNHYYWNDRATRLDGVRYLQISDENAELTRY
                     RAGALHITYVVPRGQFDWIRANLADQLHISPQLNTYYYGFNLEREPFRSKPGLRRALS
                     LVIDREKLVQRVLRVGELPAYGWIPPGVNDYTSQSFDYRDKPMAERIAEARTLFAQAG
                     YSAQHPLSFELRYNTGEVHNKVALAVASMWKDALGVDVHLTAVEFRVLLQDIDRGDVE
                     VFRSSWLGDYNDAYTFAQYLKSGFGINLPHYHSAEYDELVTSAAAQTDTSKRRELLEH
                     AERVMLADHPLVPLYFYVNKHLVKPEVQGWYDNVMNVVYSRDLGLRGQTAPSQ"
BASE COUNT         1202 a         2165 c         2191 g         1152 t
ORIGIN      
        1 gatgacttca tcgcacaggg cacccacctc ctgcatgacg tgactcgaga acagcacgca
       61 atgaccctca tcgcgcagcc tccccaatag ccggcgcaag gtccgcacgg caatcacatc
      121 aagaccgttg gtcggctcat ccagcaacac gtttcgagga cgatggatca aggcgcgcgc
      181 gagcgcggtc ttgatgcgct gcccctgcga caggccgcgg gcgggacggt cggcaaaatc
      241 ctccatctcc agcagaccga gcaactcggc cgtgcgcctg tcgcgctcgc cccggctcag
      301 gtcgtgcagg gcgccgtagt aagcgatgtt ctcgcgagcg ctcaggtgcg gatagatgcc
      361 cgcgttgtgc ggaaggacac ccatgcgtcg ccgggcttcg agcggctggg taaccgcgct
      421 gacgccgtcg atcagcgcat cgccgctctc cggtgcaatc acggtgtaca gaatgcgcaa
      481 tgtcgtggac ttgccggcac cgttcgggcc gagcagcccg gtgatgcgcc catcgagcgc
      541 ccgcagactc acatcccgca ccgccgtgac cgagccgaaa ctcttgcgca ggtgcttcgc
      601 ttcgatcacg gcgaggggcc gccgagggag gtgaagaacg gcatgggtcg gtcggcctgg
      661 gtgcacgagg catcgacccc cgcggtgccg ccggcatcga ggaaactggc catcagtttg
      721 ccgacgcacg gcgcagtgag ctggccgtgt ccgaagcctt tcaacaccac gtgcaggctg
      781 tgcgtaaagc cctgtcgagc gagctcggca tcccgcggcg gcgtgacggg atcagcgccg
      841 ccggagagca gcaggacggg ccgatcggtg tgcagggccg aatgaaaatc cggatcgatg
      901 ggcccccgcg gccagatgcg gcagatgttg cgaagaccct cgagctgagt ggtgccgaga
      961 aacgtcttct gcatctccga gatgttcacg gcggccagat catagaacgg tacatcttcg
     1021 ctgcagacga tgctgttatg cattccgtac gcgatcgcat cgcggacttc gcgcgtcacg
     1081 agcaggaact gggcggcgag aggcgtgaaa tcgcctgact gtgcggcacg gtgcagtatc
     1141 aagggcagga gcgcggcctg ctccgatgtg taggtcacaa gcctgagcac cgccgcgaag
     1201 tggtacggac tgaattcgaa ccgcacccgc tgaccgcttg tcgggtcgaa aagatccacg
     1261 ggcactgaac gggcctgcag tgagctgtgc aggctctgat aggaaagcag tggatctccg
     1321 aactgcgcgt gacagccggc atcgcttgca cagcgcgcga ggatgctcct cagcgcagct
     1381 tcggcgttgc cggccatggc aggccccaaa gcgatctgcg gcggcacgac accatccagg
     1441 atcacactgc gtacccgatc ggggtacctt cgcaggtaat gctgggcgat gcgtgtgcca
     1501 taggacacac cgtagagatt gatcgattcg taaccgagtg cgccgcgtac agcgtcgaga
     1561 tccttcactg cgatggaggt cgtgtagaac tccacgcggg ccttcggctt gaggctcgcc
     1621 agacacagct gtgtttgcgc ggcgatctgc gcatcgctcg cgcggtagag ctcggtgtca
     1681 tccagggcgc aggtgagggg attcgagtcg ccggtgcccc gctggtcgac gagcacgatg
     1741 tcgcgctcgc gatgaatcca gtcgaaggcg aaggacgccg atgcgtagaa cgccgtggcc
     1801 gccatccccg gaccgcctgc gaagacgaag agcggatcgg gccgtttgcg ccggctgatg
     1861 gccgacacac gcgccacgtg caagctgatg tggcgcccct gcggatcctg cgggttctcc
     1921 ggcacgctca acgtgccgca ctcggccgtc accgagtgca cctgcagcgg atcctggagc
     1981 tcgcaagggc tcagcacgag cgatgcggat gcctgagccc catcggcggc ggccagaagt
     2041 cccagggcaa gcagcagtga aaccgtcgga tgcgtcacgc ggggcgagag aataaagcaa
     2101 cggtgagccc gcggccttgt tcgcgcgaca cacggcccta caatacgccc cccggaagtc
     2161 ctggagggcc ggtcagggcc agttcggatg cggtttgcgc acaagttcga tgtcattgtc
     2221 gtagggggcg gtcatgccgg cacggaagct gcgctggcag ccgcgcgcat gggcgcgcgc
     2281 accttgctgc tgacccagag catcgagacc ctcggccaga tgagctgcaa tccggccatc
     2341 ggggggatcg gcaagggtca cctggtgaag gagattgatg cgctgggcgg actcatggcc
     2401 cgggccgctg atcgcgctgg catccagttt cgtacgctga actcgagcaa gggaccggcc
     2461 gttcgcgcga cgcgcgcgca ggccgaccgc cagctctatc ggcaggccgt ccggagcgct
     2521 ctcgagaatc agcctggcct tgcgttattt cagcaggagg cggcggacct cctggtcgag
     2581 ggcggccggg tcggtggggt cgtcaccgtc accggcatca ctttcgaggc gcgcgcggtc
     2641 gtgctgaccg tgggtacctt tctcggtggg cgcatccatg tgggtctgga cagctatccg
     2701 ggtgggcgcg cgggggatca gccgtcaaac cgactggccg ctcggatgcg cgagctgccg
     2761 cttcgcacag gccggctcaa gaccggtacg ccgcctcgca ttgacggccg cacgattgac
     2821 cccacggtca tgcagcgcca gcccggagat acaccggttc cggtattctc gtttcttggg
     2881 cgaggcagtg agcatccaga gcagctcgat tgtttcatta caagtacaaa cgagcgcacg
     2941 cacgcgatca tccgggctgc tacggaccgc tccccgctct tcaccggccg catcgaaggc
     3001 gtgggcccac gctactgccc gtccatcgag gacaagatca tccggttcgc ggacaagccg
     3061 tcccatcaga tcttcgtgga gcccgaagga ctgaacacgg tcgagctcta tccgaatggt
     3121 atttcgacca gcctcccgtt cgatgtgcag tacgagctgg tgcgcagcat ccggggattc
     3181 gagaatgcgc atctcactcg accgggctat gcgatcgagt acgacttctt cgacccccgc
     3241 gatctagagc cctcattgca aacgcgagtc ctggccggcc tgttcttcgc cggccagatc
     3301 aatggaacga cgggatacga ggaagcagcg gcccagggcc tcgttgccgg catcaacgcc
     3361 tgcctgatca gccgtgagcg cgaggcctgg ataccggcac gcagcgaagc ctatctgggc
     3421 gttctcatcg acgatctcgt cacctgcggt acgcgcgagc cgtaccggat gttcacgagc
     3481 cgggccgagc atcggctgct gctgcgcgaa gacaacgccg acctgcgact gactcccacc
     3541 gggcggtcgc tgggtctcgt ggacgatgag cgctgggcgc tcttcgaggc caagcaggcg
     3601 ctgtcggacc aggaagccga acgactgcag gctatccgcg tgcgccccgc tagcgtgccc
     3661 gcggattggg cgaaccgggt gctgcgctca ccactgaccc gcgagggttc ggcgttcgag
     3721 ctgctgaggc gtccggaggt gggttacgtt gacctgattg agattgccgg tgctccggac
     3781 tggacgggcg cggacgatcg attgccggtg caggttcgcg cacagcttga agtgcgagcg
     3841 aaatatgccg gctacatcga gcggcagcag gacgagatcg agcgccagcg acggaatgag
     3901 gagacacgcc ttccggccga tctggactat ctcgacgtcc cgggcctctc gcatgaggtt
     3961 cggcagaagc tgagtgagat tcgtcctgcg acagtgggcc aggcctcgcg tatcccgggc
     4021 gtcacccccg ccgccatctc catcctgctg gtgcacctga aaaagcgcgg gctggccgag
     4081 cgggcgcggg tggcatgagc cccttcctcc agcagcttgc cgcgagctgg gagcgcagtg
     4141 actctctcat ctgcgtcgga ctggatccgg agatcgagcg ctttccgccg cacgtcgctg
     4201 cgcagccctc gccgatcttc cagttcaaca gagcaatcat tgatgcgacc gcggatctgg
     4261 tctgcgccta caagccgcag ttcgcgcact acgcggccta cgaagcggaa gaccagctcg
     4321 agcgcacgat cgaatacatc caccgcagct acccgggcgt tccagtcatc ctcgacagca
     4381 agcgcggcga tgtgggcaat acggcggagc gctatgccat cgaggccttc gagcgctatg
     4441 gcgccgatgc cgtgacggtg aatccatatc tcggcgccga ttccgtcgag ccgttcctga
     4501 agtacacgga taaaggcgtc atcgttctct gccggacctc caatcccagc agccgcgacc
     4561 ttcaggatct ggaggtcggc gggcgcaagc tgtaccaggt cgtcgccgag atggtctgcc
     4621 gcaagtggaa ctcacgtggc aattgcctgc tggtggtggg cgccacttat ccacgggagc
     4681 tcgcggaggt ccgcgagatc gccggcagca tgccgtttct ggtgcctggt gttggggcgc
     4741 agggcgcgga cgtggcgcag gcggtgctga atgcgcagac gcccgatggg accgggctga
     4801 tcatcagctc ttcgcggggg atcctctacc gatccctggg cgatgacttc gcggcggcgg
     4861 cgcgctcggc tgcagagaag cttcgcaatc aggtcaacga gagcagatcg cggcgtgcgc
     4921 gctgaggctg ctcgcgcctt cagccggcca atgcacgtgc tgtgtgccaa tcggctcgcg
     4981 ctgattgcag cactgatctt tgccctgagc gttctgaccg ttgccggctg ctcgcgcaat
     5041 ccggtctcag gctcggccaa cgggtccggc gctgccatcc ttctgcgcgg cggcggcccc
     5101 gagcccgact cgctcgaccc tcagaaggcg cgcgcggtag agtcgcaggg catcctgcgg
     5161 gatctgtgcg aaggcctgac caccctggcg aaggatgcca gtacggcccc tggagtgtcc
     5221 gatcaatgga ccgtcagcgc cgatggtcgg agctacacat tcaagcttcg ccccaacgca
     5281 cgctggtcca acggcgaccg cgtggtcgcg gcggatttcg tcgccgcgct gcgccgcctg
     5341 gtcgatccgg cgacggcttc gccttatgcg caggtcatcg acatcatcac caacgccagt
     5401 gacatcgtcg ccggcaagaa gccgccggag tctctcggag tgacggcacc cgatgaatcc
     5461 accgtaagca tccagctggc ggccccggcg acgtatcttc cggggctgat ggcgcatccg
     5521 agtacgtgtc cagtccatcg gggcacgctc gctcagcacc cttccgagct cgcccggccc
     5581 ggcgtcatgg tctccaatgg cgcgttctat ctgaaggagt gggtgcaagg ttcccacatc
     5641 ctgctgctgc gcaaccatta ctactggaac gatcgcgcta cgcgcctgga cggcgtgcga
     5701 tatctgcaga tctccgacga gaatgccgag ctcacccgtt atcgcgccgg cgccctgcac
     5761 atcacctatg tggttccccg gggccagttc gactggatcc gcgccaacct tgccgaccag
     5821 ttgcacattt cgcctcagct caacacgtac tattacggct tcaacctcga gcgcgagccc
     5881 ttccgcagca agcccggcct gcgccgggcg ctgtccctgg tcatcgatcg cgagaagctc
     5941 gtgcagcgcg tgttgcgcgt cggcgagctg ccggcctacg gctggattcc gccgggagtg
     6001 aacgactaca cctcgcagtc cttcgactat cgcgacaagc ctatggccga acgcatcgcc
     6061 gaggcgcgca cgctctttgc gcaggcgggt tattcggccc aacatccact cagcttcgag
     6121 ctgcgataca acactggaga agtgcacaac aaggtggctc tggcggtcgc ttccatgtgg
     6181 aaggatgctt tgggagtcga cgtgcacctc accgcggtcg aattcagggt cctgttacag
     6241 gacatcgatc gcggcgatgt cgaagtcttc cgctcgagct ggctgggcga ctacaacgat
     6301 gcctacacgt tcgcgcagta tctcaagagc gggttcggca tcaatctgcc gcactaccac
     6361 agcgccgagt acgatgagct ggtcacatcc gcagccgccc aaacggatac atcgaagcgg
     6421 cgcgagctgc tggagcatgc agagcgcgtg atgctcgcag atcatcccct ggtgccgctc
     6481 tacttctacg tgaacaagca cctggtgaaa cccgaggtgc agggctggta cgacaatgtc
     6541 atgaacgtcg tctacagcag ggacctgggc ctgagagggc agaccgcccc gtctcagtga
     6601 gtgccgcgac ttggcctgcg gcgcagggcg ccaaagtcgc gcctttggcg cccggcatcg
     6661 gagctgacgt ctgacaaagc gccgcaggcg gctttttcca atgaacccgc
//
LOCUS       DASRHN010000393        10036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_634787, whole genome shotgun sequence.
ACCESSION   DASRHN010000393 DASRHN010000000
VERSION     DASRHN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634787"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..256)
                     /locus_tag="VFB37_15885"
     CDS             complement(14..256)
                     /locus_tag="VFB37_15885"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZO23979.1"
                     /translation="MGTWQHSARRVSLLAASALIAPSLLIAPAVIAGWGLWLRQRVGG
                     MTGDCLGAGVEVSEVVLLLVVGFVHPAGLAVAASWR"
     gene            complement(669..1073)
                     /locus_tag="VFB37_15890"
     CDS             complement(669..1073)
                     /locus_tag="VFB37_15890"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZO23980.1"
                     /translation="MSAAPLTGNCLCGALRYECGQPVSAAAFCHCQSCRRAAGAHVVV
                     WMTVSLASFRFTGGTPAQYASSPPVIRQFCARCGSQITYWHEKAPDTIDVTVATLDRP
                     GMIPPADHIWMSDAVAWDQITDGRPAHPAGRT"
     gene            complement(1079..2764)
                     /locus_tag="VFB37_15895"
     CDS             complement(1079..2764)
                     /locus_tag="VFB37_15895"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZO23981.1"
                     /translation="MRFNSLTARYLREMLALTPVSATVLGEHRYDAMLDDVSAAGFTR
                     RAQLARELLAELQPLDLSKLSRAHQVDARLLRNELEYQIWSVEQLAEWRWNPLIYTDL
                     AGNSVYLLMARDFAPLQQRLHSVGARLSELPHFLEQVRTSLDPARVPRIHAETAIKQN
                     SGILALIDQLVIPQLATLAEPEQAVLKGAIATARTAVAQQQIWLERKLLPAAQGNFRL
                     GAALYDSKLAFALDSPLSRQEIRTRAEAELTRTRDEMYSIARTALAGQPNAPPLPVTP
                     GPDEQQAAIVAALELAYAQQPQRDQVFDAVQTALGEARRFVVSSGLVTLYDDPLEVIP
                     MPEFQRGVALAFCDAPGPLDRDQKTFFAVSPIPADWTVGQVNSYLREYNTRSIYELTI
                     HEAMPGHYVQLSHANRYDSQLRAVLGSGTFIEGWAVYAERMMVEQGFHGSDPLMHLIQ
                     LKWYLRSVANAILDQAVHVDGISREQAMTLMTHDTFQEEREAAAKWVRVELTAAQLPT
                     YFVGVQEHLALREEARRVWGKGFTLRRYHDSVLAYGSPPVRYVRELMLDLPIQ"
     gene            2936..5389
                     /locus_tag="VFB37_15900"
     CDS             2936..5389
                     /locus_tag="VFB37_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO23982.1"
                     /translation="MGSEAQRLDGHPGRARLQNTPEGVDAAGFRLLAESLPHPVWVCG
                     PSGTLQYMNRCGLQYFGSHLHQPATPFPSGSLAHPDDSAISRESWSHACADGEAFNLR
                     MRLHRSDGAFRWHSLDTQPIRDTTGYVTGWLGAATRMDASKRDDPSAFLLALSTDVAR
                     MDDPHEMICEVMLRLRQRLGAGQVALAEIDEPREEAIVLRQDRNDPSTLQLVSLPLEV
                     LGHSPFDTGKQEMGVISDTSTDPLAASRYAQCCGLEGVGAIVSAPLRRAGALVAVLII
                     GESSPRDWRTSEIELIRRVAELVWPALEKARAERALALSEQRLRLAQAVTRIGAWELD
                     PRAGSISFSTESYELFGLQEAGAADLYERWMRRVDARDRAVLSALVRECLGSGKAHAE
                     YRYFHPQRGERWIYCRAGAVKESGRACIVGVSLDVTERRRAEEALKDVNRRKDQFLAM
                     LGHELRNPLAPIRNAVQILSSHSGGSPEIEWAHKVLERQTRHLTRLVDDLLEVARAVR
                     GQIVLRTAPLQLSEWVQNAIETTQPLIGARRHRLHVSLPAELVQLRGDLLRLSQALAN
                     LLNNAARYTEAGGEIWLDARLQAGEVELRVSDTGPGLSADLLPHVFDLFTQSERPLDR
                     AQGGLGIGLTLVKLLIEMHGGSVEARNSQRHPGAEFIIRLPAQKVVEAERDCSCGCES
                     APSPDPHLKPETERSMRVLIVDDNVDAAESIALLLSLDGFDARTVHSATEVFEVVAAF
                     TPDVILLDIGLPVMDGYEVARELKARFLHHKMRIVALSGYGQPADRRRAAQAGFDDYL
                     VKPVEAARLSALLRKQPES"
     gene            5451..6536
                     /locus_tag="VFB37_15905"
     CDS             5451..6536
                     /locus_tag="VFB37_15905"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZO23983.1"
                     /translation="MSDDAGRRKSFQGRGALSNPAGRFESCAVLAVDDGWYVEDQPDS
                     IATSVEPDRARGIISTNDSPDIGFEQSINPYRGCEHGCIYCYARPSHAYMGLSAGLDF
                     ETRLFYKAEAASVLQSELARPGYVCKPIMLGANTDPYQPVERRMRITRSILEVLSATR
                     HPVSVITKSASVLRDLDLLTNLARANLTGVSVSITSLEPETKRTLEPRAASPQARLAA
                     VRKLTAAGVPVSVMVAPVIPAVTDHELEAILEAAAGAGALWAGYVMLRLPYEVKDLFR
                     EWLAQHHPQRAAHVMSLIQDLRGGRDNDPRFGTRMKGTGPLAELIGNRFRIACRRHGL
                     NAKRRPALNTTLFRPPLIEGAQMSLRF"
     gene            6711..7214
                     /locus_tag="VFB37_15910"
     CDS             6711..7214
                     /locus_tag="VFB37_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO23984.1"
                     /translation="MRRESCVALITALLCGMGGCASKPLTPAPQPLQAAQQQTVAAAG
                     STSAALGLKAAAKTAPASANSGNGGTGSAAASVATVKVPLDAGATSQTPPVVAIAPAA
                     TGVARRASGGGYLGGNGSTAHEVGDGSDDDIIARRLRKAVENETDPDLKVRLWKEYVD
                     YRNGTQK"
     gene            7443..>10036
                     /locus_tag="VFB37_15915"
     CDS             7443..>10036
                     /locus_tag="VFB37_15915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO23985.1"
                     /translation="MTEAVASFQGRKLAARYTLVEKLGAGGQGEVWRAHDGTRGVDIA
                     LKVLNPAQMGDEEAWAALEHEYAIASLLDHPAILKVYPPDRSGDVIVLPMELASGGDL
                     RRLRGAGFLEIIPVLLEVAQALEHAHDRGVVHRDLKPGNVLFDARGRARLADFGVAGA
                     AARKTASENPTTPVPSVDPNRQGLSPFTASPAQLRGEPPKPADDIYGLGALAYELLSG
                     YPPYYPHFDAKRAIEEPVPELVPTRQIPPLLASLVRRMLAKDAAQRPRSMREVIDELD
                     LALNSTLTYDFDSVAESLRAAATRARIESSDSAAGGPSNATDAIPPPRAGSVDLAALF
                     RHATPAANMPEGPPRVPEWSPGAGLDRRGASDRRARRTSFLPDTDIRPSGLALPGAAR
                     ERPEIGERQARIAERQDRTVQRRAESDVQAEAGHRSVSSIPKPLPRARLEPQPRLADE
                     PQPVPEPRQVAVPPWRAIPLDGLPRLPRLEPIRHRRWPWLLLGLIAGGAAAVYFWLPQ
                     VDLRALPPDLATLVQPLQAALQSDGAQRQPAPEVVTQAEAPVAAQATPDETQATPEQD
                     SKSRSRAAAAPQLAPAPQLAPAPQASPKSGREARFRAQRKALESRVAMLEDRGAGAWG
                     GRDFAMARTRADEAVRANDAGDMRVAQERMATASRLLDTVEANAPQAFVAQIAAGEKA
                     LTTGQQEVAGQAFDLARRIDPNDRRASDGQHRAQSLGGVLPLLADAQNAESAHDYARA
                     AQDYSQALSLDPGNASAKAGQARSNAAFGEDNYAKMVGAGFAALGAGRLEEARDDFVK
                     ARALRPRGPEAAEGMRRVSAALTLRGYASMRQRASSLEAQERWDEAEQVYDDALKADP
                     SLAFAQEG"
BASE COUNT         1753 a         3210 c         3387 g         1686 t
ORIGIN      
        1 gtttatcgcc cgctcagcgc caggaggctg ctacagccaa ccccgcagga tggacgaagc
       61 ccactacgag caacagcacg acttcgctca cttcgacccc cgcaccgagg caatctccgg
      121 tcatgccgcc gacacgctgg cgcagccaca gtccccagcc ggcgatcact gcgggggcaa
      181 tcagcaggct gggtgcgatc agggcactgg cggccagcag cgatacccgc ctggctgaat
      241 gttgccatgt tccgatgcag cgtcagggcc gcccgtatga tcggcagtgt cagtgacgcc
      301 cccgtgcctt cgcgcaaccg cattccgagg tcgatcagag gctcgagctg cagcgctcgc
      361 aatgcgcggt ggtgttcgcg atgccatgtc gccggcaatc aatagacgag cgccgcgctg
      421 aacagcgagc ggtgaccgct tgcggaaagc cgacacgccg cgcgcggcca cgccctgatc
      481 ggatgcgaac acgaagatct cgcaaggggg catcacgggt acaggattgc gcagccgcgc
      541 ggcgaaccag caggccagat cctcgagacg tccgagcgag ccggggggct tggtgagctg
      601 ttcctgacgg gcgcgtgctg cctcgcccgc tgccgcgtcc ggtgccggcc tcgaatgcca
      661 tcccgcgctc aggtgcgccc cgcgggatgc gctggccggc catcggtgat ctggtcccag
      721 gccaccgcat cgctcatcca gatgtgatcg gcgggcggga tcataccggg ccggtcgagc
      781 gtggcgaccg tcacatcgat ggtgtccgga gccttctcgt gccagtacgt aatctgcgat
      841 ccgcagcgag cacagaactg ccgtatcaca ggcggcgacg acgcatattg tgcaggcgtc
      901 ccgccggtga accggaagga tgcgagcgaa acggtcatcc acacgacgac gtgcgctcca
      961 gccgcccgcc ggcaggattg gcagtgacag aacgccgccg cggataccgg ctggccgcat
     1021 tcgtagcgca gcgctccgca gagacagttt ccagtgagtg gcgcagcgga catgatcgtc
     1081 actggatcgg caggtcgagc atcagctcgc ggacgtaacg cacagggggc gagccgtatg
     1141 caaggaccga atcatgatag cgcctgagtg taaagccctt gccccacact cggcgcgctt
     1201 cctcgcgcag ggccagatgc tcctgcaccc ccacgaaata cgtcggcagc tgcgccgcag
     1261 tgagctctac gcgcacccac ttcgcagccg cttcccgctc ctcctggaac gtgtcgtgcg
     1321 tcatcagggt catggcctgc tcgcgggaga tgccatcgac gtgcaccgct tgatcgagaa
     1381 ttgcattggc gacgctgcgc agataccact tgagctggat caggtgcatc agcgggtcgc
     1441 tgccgtggaa accctgctcg accatcatcc gctcggcgta cacggcccac ccctcgatga
     1501 acgtgccgga gccgagaact gcccgaagct gtgagtcgta acgatttgcg tgtgagagct
     1561 gcacgtagtg gccgggcatc gcctcgtgga tggtcagctc atagatcgag cgggtgttgt
     1621 attcgcgcag gtacgagttg acctggccca cagtccagtc cgcggggatc ggcgagacgg
     1681 caaagaaggt tttctggtct ctgtccagag gccccggcgc atcgcagaag gccagggcca
     1741 ctccgcgctg gaactcgggc atcgggatga cctccagcgg atcgtcatag agtgtgacga
     1801 ggccggagga gaccacgaag cgcctggcct cgccgagcgc cgtctgcacg gcatcgaaga
     1861 cctggtcacg ctgaggttgc tgtgcgtacg ccagctccag agcggcgacg atcgcggctt
     1921 gctgctcatc gggtcccggt gtcacgggca gaggcggtgc attgggctgc cccgcgagcg
     1981 cggttctcgc gatggaatac atctcatcgc gcgtgcgggt cagctcggct tcggcacgcg
     2041 tgcggatttc ctggcgcgaa aggggagagt ccaatgcgaa cgcaagcttg ctgtcgtaga
     2101 gcgccgcccc gagccggaag tttccctggg cggccggaag cagcttcctc tcgagccaga
     2161 tctgctgttg agcgacggcg gtgcgcgccg tggcgatagc tcccttgagg actgcctgtt
     2221 cgggctcggc gagcgttgca agctgcggga tcacgagctg gtcgatgagc gcgaggatgc
     2281 cgctgttctg tttgattgcg gtttcggcgt ggatgcgcgg cacccgggct ggatcgagcg
     2341 aggtgcggac ctgctcgaga aaatgcggca gctccgaaag gcgcgcgccg acgctgtgca
     2401 gcctttgttg cagcggcgcg aagtctcgcg ccatgaggag gtaaacgctg ttgccagcca
     2461 ggtcggtata aatgagcggg ttccagcgcc actccgccag ctgctccacg ctccagatct
     2521 gatattccaa ctcattccgc agcaaccgcg cgtcgacctg atgcgcccgt gagagcttgc
     2581 tcagatcaag cggctggagc tctgccagca actcacgggc gagctgcgcg cggcgcgtaa
     2641 atccggccgc gctcacatca tccagcatcg catcgtagcg atgctcgccg agtactgtgg
     2701 cgctgacagg cgtgagcgcc agcatctcgc gcaaatagcg cgctgtcagg gagttgaaac
     2761 gcatgtcagc ctctgtcggt gacgaaggcg ctggagtcgg tggcgtagca gcgcatccgg
     2821 caaagcccac gattgccgcg cacagcatcg agggaatgaa ggtacggcga ttgcttctct
     2881 gctcttccat cggaccccca aagcctgggg cgcatgatga ccaagaccga gtggcatggg
     2941 aagcgaagca cagcgtctcg acgggcaccc gggacgcgca cggttgcaaa acacgccgga
     3001 aggggttgat gccgcaggct ttcggctgct ggccgagagt ctgccgcacc ccgtgtgggt
     3061 gtgcggaccg agcggcaccc tgcaatacat gaatcgctgc gggctgcagt actttggatc
     3121 ccaccttcac cagcctgcca cgccgtttcc atccggctcc ctggcgcatc cggatgacag
     3181 cgcgatctcg cgcgagtcgt ggagccacgc ttgtgcagac ggcgaagctt tcaatctacg
     3241 gatgcggctg catcgctccg acggtgcgtt ccgctggcac agtcttgaca ctcaacctat
     3301 ccgcgacacg acaggatacg taacaggttg gttgggcgct gcgaccagga tggacgcgag
     3361 caagcgtgac gatccgagcg cctttctgct ggcgctctcg accgacgtgg ctcggatgga
     3421 cgacccgcat gagatgattt gcgaagtgat gctccggttg cgccagcgcc tgggggctgg
     3481 gcaagtcgcg cttgccgaga tcgacgagcc acgggaggag gcgatcgtcc ttcgccagga
     3541 ccggaatgat ccctccacat tacagctggt cagcctgccg ctcgaagtcc tggggcactc
     3601 gcctttcgat accggcaagc aggagatggg cgtcatatcc gacacgtcca cagatccgct
     3661 ggccgcctcc cgctacgccc agtgctgcgg gctcgagggg gtcggggcga tcgtgtctgc
     3721 accgctgcgt cgggccggcg cgctggtcgc tgtgctcatc atcggcgaaa gctcgccgcg
     3781 cgactggcgc acgtccgaga tcgagctgat tcgtcgtgtc gcggagctag tctggccggc
     3841 tttggaaaag gcgcgtgcgg agcgggcact ggctctcagc gagcagcgct tgcggttggc
     3901 gcaggcagtg acacggatcg gcgcgtggga gctggatccg cgcgcaggct cgatcagctt
     3961 ttccaccgag agctacgagc tgtttggcct gcaggaggcg ggggcagcgg atctgtatga
     4021 gcgctggatg agacgggtcg atgcacgtga ccgcgccgtt ctgagtgctc tggtgcgcga
     4081 gtgcctcggc tcgggcaagg cccatgcgga gtaccgttac tttcatccgc agcggggcga
     4141 gcgctggatc tactgcaggg caggggcagt gaaggagagc ggccgtgctt gcatcgtcgg
     4201 cgtcagtctc gatgtgaccg aacggcgccg ggcggaagag gctctgaagg atgtcaaccg
     4261 tcgcaaggat cagtttctgg ccatgctggg tcacgagttg cgcaacccgc tcgcgccgat
     4321 ccgcaatgcc gttcagatcc tgagctcaca ttccgggggc tcgcctgaaa tcgagtgggc
     4381 gcacaaagtg ctcgagcgtc agacccgcca cctcacgcga ctcgtggacg atctgctgga
     4441 agtggcacgc gccgttcgcg gacagatcgt cctgcggacc gcgcccctgc agctgtcgga
     4501 gtgggtgcag aacgcgatcg aaaccacaca gccgctcatt ggcgcacggc gccatcggct
     4561 tcatgtgagc ctgcccgcgg agctcgtaca gctccgcggc gatctgctgc ggctctccca
     4621 ggcgctcgcc aacctgctca acaacgccgc ccgctacacc gaagccggcg gcgagatctg
     4681 gctggacgcg cgccttcagg cgggcgaagt cgagctgcgc gtgagcgata cggggccggg
     4741 cctgagcgcg gatcttctgc ctcacgtctt cgatctgttc acgcagtccg agcggccgct
     4801 cgaccgcgcg cagggcggac tggggatcgg tctcacgctg gtgaaattgc tgatcgagat
     4861 gcacggcgga tcggtcgagg cccgcaactc gcagcgccat ccaggagcgg aattcatcat
     4921 ccggttaccc gcgcagaaag ttgtcgaggc cgagcgtgat tgctcctgcg gctgcgagtc
     4981 cgcgccctca cccgatcccc atctcaagcc ggagaccgaa agatcaatga gagttttgat
     5041 agtggacgat aacgtcgacg ctgccgagag catcgcgctg ttgttgagtc ttgacggatt
     5101 cgatgcgcgc actgtgcaca gcgctacgga ggtgttcgag gtcgtcgccg cattcacgcc
     5161 cgacgtgatt ctgctcgata tcgggctgcc cgtgatggac ggttacgagg tagcacgcga
     5221 actgaaggcg cgctttctcc atcacaagat gcgcatcgtc gctctgagcg gttacggaca
     5281 acctgcggac cgccggcgtg cggcgcaagc ggggtttgac gactatctgg ttaagccggt
     5341 cgaggccgcc cgcctgagcg cgctgctgcg caagcagcct gaaagctgac acgatcctct
     5401 ccccgacgcg gccgctttac ctctaggctg tatgggcgta cagtattgcc atgagcgacg
     5461 acgccgggcg gcgcaagtcc tttcaggggc gcggcgcgct gtccaatccg gcggggcgct
     5521 tcgagtcctg tgccgtcctc gccgtggacg atggctggta tgtcgaggat caacccgaca
     5581 gcattgccac cagcgtcgag ccggaccggg cccggggcat catcagcacg aacgactccc
     5641 ccgatatcgg attcgagcag tcgatcaacc cctaccgggg ctgtgagcat gggtgcatct
     5701 actgctacgc ccgtcccagc catgcttaca tgggcctgtc cgcgggcctc gacttcgaga
     5761 cgcggctctt ctacaaggcc gaggcagcca gtgtgctcca gagcgagctg gctcggccag
     5821 gctatgtctg caagcccatc atgctcggag ccaacaccga tccgtatcag cccgtcgagc
     5881 gccgcatgcg catcacgcgc tcgatcctgg aggtgctgag cgcaacccgg cacccggtat
     5941 cggtcatcac caagagcgcc tcggtgctgc gcgatctgga tctgctgacg aatctggctc
     6001 gcgcgaatct tacgggagtc tcggtcagca tcacttcgct cgagcccgag acgaaacgta
     6061 cgttggagcc acgagcagct tcgccccagg cgcggctcgc agcggtccgc aagctaacgg
     6121 cggctggcgt gccggtcagc gtgatggtgg cgcccgtcat tcccgccgtc accgatcatg
     6181 agttggaagc catcctggaa gcggccgcag gcgcgggcgc tttgtgggcg ggatatgtga
     6241 tgctgcgttt gccctatgag gtcaaggatc tgttccgcga gtggctggcg caacatcacc
     6301 cccagcgcgc cgcgcacgtg atgtcgctga tccaggacct gcgcggaggt cgggataacg
     6361 atccgcgctt tgggacacgc atgaaaggga caggtccgct cgccgagctg atcggcaatc
     6421 gattccggat cgcttgccgg cggcacggtc tcaatgcgaa gcggcggccc gcgctcaata
     6481 ccactttgtt tcggccgccc ctgatcgagg gggcccagat gagcctgcgc ttctgattca
     6541 ccgacgggcg cggaaccgaa cttcactgcc gatcatccaa tacctgccgc gagtggcata
     6601 ctcgagcaga agctgctggt ttcagtggaa cgacccctga atcaagcatt cccgccgtca
     6661 ggtctaccgg caccggttga gcttatgtca actggaaata ggcacgaacc atgcgtagag
     6721 agtcctgtgt cgccctcatt actgccttgc tgtgcggaat ggggggctgt gcgagcaaac
     6781 cgctgacacc ggcgccgcaa cctttgcagg cagcgcagca gcagaccgtc gcggcggccg
     6841 gaagtacgag cgctgccctg ggcctgaagg cggctgcaaa gactgcccca gcctcggcca
     6901 actcaggcaa cggcggtact gggtcggccg cggcgtcagt cgccaccgtc aaggttccgc
     6961 tcgatgccgg cgcgacatct cagacgccac ccgtggttgc gattgcaccg gcggccacgg
     7021 gggtggctag acgggctagc ggaggcgggt acttaggcgg aaacggttcg actgcacacg
     7081 aggttggcga cggcagcgat gacgacatca tcgcgcggcg gttacgcaag gccgttgaga
     7141 acgagaccga tccggacctc aaggtcaggc tctggaagga gtacgtggac tatcgcaacg
     7201 gtacgcagaa gtgaatggga gttgcagcga atcggctgta agcggttggg gcgagtccga
     7261 cgttattagt ggtttggaag gcagccgatc gggagaatca gtgcgtgatg taagacccac
     7321 aggctcggct tggcggctct ccccaaagtg gagttcgtgc cgccgcgcag atccggcaaa
     7381 atgatcgacc ccggagcgcg gctccgcggc cacgatcaag gattgcgagc tggtgtgaag
     7441 acatgaccga agcagtggct tcgtttcagg gccgaaagct ggcggcgcgc tacacgctgg
     7501 ttgagaagct gggcgcgggc ggccagggag aggtctggcg cgcgcacgat ggcacgcgtg
     7561 gcgtcgatat cgcgctcaag gttctcaacc ccgctcagat gggagatgag gaggcgtggg
     7621 cggcgctcga gcacgagtac gccatcgcga gccttctcga tcatccagcc atcctcaagg
     7681 tctaccctcc ggatcgctcg ggcgatgtga tcgtgctgcc gatggagctg gcctccggcg
     7741 gtgatctgcg ccggctgcgc ggagcaggct ttctcgagat catccccgtt ctgctcgaag
     7801 tcgcgcaggc gctcgagcat gcgcacgatc gaggcgtcgt gcaccgggat ctgaaaccgg
     7861 ggaacgtgct gttcgatgca cggggccggg cgcggctcgc cgatttcggc gtggcagggg
     7921 ccgcggcgcg aaagactgcg tcagagaatc cgaccactcc ggtcccctct gtcgatccca
     7981 accggcaggg tctctctccg ttcacggcca gtccggccca gttgcgtggc gagccgccca
     8041 agccggccga tgacatctat ggccttggcg cgctggctta tgagctgctg tctggctacc
     8101 cgccttacta tccgcacttc gatgccaagc gcgcgatcga agagccggtg cccgagctcg
     8161 tgccgacgcg gcagatcccg ccgctgctgg cctccctggt ccggcgaatg ctggccaaag
     8221 acgcggcaca gcgcccccgc tcgatgcgcg aagtcatcga cgagctggat ctggccctga
     8281 attccacact gacttacgac tttgacagtg tggccgagtc gctgcgcgcg gcagccaccc
     8341 gggctcggat cgagagttcg gatagcgcgg ccggcgggcc gtcgaacgcg actgatgcca
     8401 taccgccgcc gcgcgcgggg agtgtggatc ttgcggctct cttccggcac gcaacccctg
     8461 cggccaacat gcccgaaggg ccgccgcggg taccggaatg gtctcccggg gcggggttgg
     8521 atcgtcgcgg cgcgtcagac cggcgggcgc gacgcacctc gttccttcct gacaccgata
     8581 tccgaccgtc ggggctggcc ttgccaggcg cagctcgtga gcggccagag atcggcgagc
     8641 gacaggcgag aatcgctgag cggcaggacc gaacggttca gcggcgagct gagagcgatg
     8701 tccaggcgga ggcgggacac cgttccgtgt catcgatacc gaagcccctg cccagggcgc
     8761 gccttgagcc gcagccgcga ctggccgatg agccgcagcc cgtgccagag ccgcggcaag
     8821 tcgcggtacc cccgtggcgt gctattccac tcgatggctt gccccggctt ccgcgcctgg
     8881 agccgatccg ccaccgccgt tggccctggc tgctgctcgg gctgatagca ggcggcgcgg
     8941 ccgccgtgta tttctggctg ccgcaagttg atctgcgcgc gctgcccccg gatctcgcca
     9001 ctttggtcca accgttgcag gcggcgttac agtcggatgg cgcgcagcgc cagcccgcgc
     9061 ccgaagtggt cacccaggcc gaggcacctg ttgctgcgca agcgaccccc gacgagacac
     9121 aggcgactcc cgaacaggat tcgaagtccc gttctcgggc agccgccgcc ccgcagctcg
     9181 cgccggcccc gcagctcgcg ccggccccgc aggccagccc gaaaagcggc cgcgaagccc
     9241 gtttccgggc gcagcgaaag gccctcgaga gccgcgttgc gatgctcgag gaccgcggcg
     9301 ccggcgcatg gggcgggcgc gatttcgcga tggccagaac gcgcgctgac gaggcggtgc
     9361 gcgcgaacga tgcgggggat atgcgggttg cacaagagcg catggcaacg gcatcgagac
     9421 tgctggacac cgttgaagcc aatgcgcccc aggctttcgt tgcgcagatc gcagcgggcg
     9481 agaaagcgct cacaaccggt cagcaggaag tcgccggtca ggcgttcgat ctggcacgcc
     9541 gtatcgaccc gaacgaccgc cgggcctccg atggccagca tcgggcgcaa agcctcggcg
     9601 gcgtgctgcc gctgctggcc gatgcgcaga atgccgaatc tgcccacgat tacgcgcgcg
     9661 ccgcacagga ttacagtcag gctctgtcgc tcgatcctgg caatgcttcc gccaaggcgg
     9721 ggcaggcccg ctcgaacgcc gctttcgggg aagacaacta cgccaagatg gtcggcgcgg
     9781 gattcgccgc tctgggcgcg ggtcgtctcg aggaggcccg cgatgatttc gtgaaggccc
     9841 gggcactcag gccgagaggg ccggaggccg ccgagggtat gagaagggtc agtgcagcgc
     9901 tgacccttcg cggctacgcc tcgatgcgtc agcgtgcgtc gagcctcgaa gcgcaagagc
     9961 gctgggatga agccgagcag gtctacgacg atgctctgaa ggcggatccg tcgcttgctt
    10021 ttgcccagga gggcaa
//
LOCUS       DASRHN010000394         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1959624, whole genome shotgun sequence.
ACCESSION   DASRHN010000394 DASRHN010000000
VERSION     DASRHN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959624"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VFB37_15920"
     CDS             <1..1590
                     /locus_tag="VFB37_15920"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZO23986.1"
                     /translation="DDFINRKHGRSEGPIDYLHPSLKTVMEPTYGVILYQEQVMQIAQ
                     VLAGYTLGGADLLRRAMGKKKPEEMAKQRSVFVEGAVARGVAPPLAAHIFDLMEKFAG
                     YGFNKSHSAAYALLSYQTAYLKAHYPAAFMAAVLSADMDHTDKVVTLIMECADIGLQV
                     KPPDVNQSSYEFAAGTERTIRYGLGAVRGVGRGAVDALIAEREAGGAYTSIEDLCRRL
                     DLQKVNRRVLEALIRCGSLDGLGANRAALMQRLPAAMQLGDQNSRAHEAGQNDLFGLA
                     GDGPAARPLAVRSVDPPEWSEAVRLSGERETLGLYLTGHPIAQFEAGLTQLITHRIGD
                     LVSDRPMEPGRFSGGRAVAVAGLIDEVKKRGPRVILRLDDRTGRLEVTLFEDVFQRHR
                     DLVAKDALVLVEGMLRFDEFSDSWRLSARRITELDKVREQQARRIVLKWPPHGDSASL
                     LRRLREILAPWRGGSCPVTVEYSGSGARGALTLGPEWMVKAGRELLEKLEGLLGRGGV
                     QVVYGSAPSAASTPTVSADSG"
     gene            1695..2660
                     /locus_tag="VFB37_15925"
     CDS             1695..2660
                     /locus_tag="VFB37_15925"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZO23987.1"
                     /translation="MAVAFLDFEQPIAELEAKIEELRHVTSESEVNILDEINRLQLKS
                     QQLTSSIFANLTPWQITQLARHPHRPYTLDYVNSIFSEFTELHGDRMFADDLAIVGGL
                     ARLDDRALMIIGHQKGRDTKERVRRNYGMPKPEGYRKALRLMRLAERFGLPLLTLIDT
                     QGAYPGVGAEERGQSEAIARNLFEMSVLSVPIVTVVTGEGGSGGALAIGVCDRLLMLQ
                     YSTYSVISPEGCASILWKSQDKKEAAAEALNLTADRLLKLSLVDEVIEEPLGGAHRDP
                     IETAHRLKAAIVRHLDQLEAMPRDELRAARSAKIASFGLFSENPE"
     gene            2747..4060
                     /gene="tilS"
                     /locus_tag="VFB37_15930"
     CDS             2747..4060
                     /gene="tilS"
                     /locus_tag="VFB37_15930"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZO23988.1"
                     /translation="MALSGGLDSTVLLAALAGARRRSVRLRAVHVNHGLHPNAALWSG
                     HCERLASALDVPLTILTGRVMRGRGVSLEAAARETRYRLLAAILTPGEFLLSAHHEDD
                     QLETVMLQLFRGAGLAGLAAMPALTAFGPGWLARPLLPCTRMQLEEWGRRSELSWVED
                     DTNTDERLDRNYLRRRILPLVRDRWKGAASAVSRSARHAAEGQRLLDALARKDVEQAA
                     FGTELSVRSLRALSADRRRNALRFWIAHSGRMLPDTRRLDELAGPMIDARPDANPVVR
                     WADTLVQRHTDLLWIRPSPKPLPGEGAGRRKLRAEVRGRPIARSTHWSWRASSAIDLP
                     DRCGKLELRPDPHGPVDLDVLPESVSIRWREGGERIRPRRGGPRRALKSLLQEARLPL
                     ALRASVPLIFDGDTLLAVGELWLDESIQATAAAPHRARLLWSPQS"
BASE COUNT          676 a         1391 c         1476 g          716 t
ORIGIN      
        1 gatgacttca tcaatcgcaa gcacggtcgc agcgagggac cgatcgacta cctgcacccg
       61 agtctcaaga cggtgatgga gccgacctac ggcgtcatcc tgtatcaaga gcaggtgatg
      121 cagatcgccc aggtgctcgc cggctacacg ttggggggcg ccgacctgct gcgccgggcg
      181 atgggcaaga agaaaccgga ggagatggcc aagcagcgct ccgtgttcgt cgagggtgcc
      241 gtggcgcgcg gggtggcccc gccgcttgcg gcgcacatct tcgatctgat ggaaaagttc
      301 gccggctatg gattcaacaa gtcgcactcc gccgcctatg cgctgctgtc gtaccagact
      361 gcgtacctga aggcacacta tccggctgca ttcatggcgg ccgtgctctc cgccgacatg
      421 gatcacaccg acaaggtggt cacgctcatc atggagtgcg ccgacatcgg gctgcaggtg
      481 aagccaccgg atgtcaacca gtccagttac gaattcgcgg ccggaacgga gcgcaccatc
      541 cgctatggcc tgggcgcggt gcgcggcgtg ggccggggcg cggtcgatgc gctgattgcc
      601 gagcgcgaag cgggcggagc ctacaccagc atcgaggatc tgtgtcggcg cctcgatctg
      661 cagaaggtca accggcgcgt cctcgaggcg ctgattcgct gcggcagtct ggatgggctc
      721 ggagccaatc gtgccgcgct catgcagcgc ctgcccgcgg cgatgcagct cggagatcag
      781 aattcgcgcg cgcacgaggc gggacagaac gacctgttcg ggctcgcggg cgatgggccc
      841 gcggcgcgcc cgctcgccgt gcgctcggtc gatccgcccg agtggagcga ggcggtacgc
      901 ctgtccggag agcgcgagac gctgggcctg tatctgaccg ggcatcccat cgcgcaattc
      961 gaggccgggc tcactcagct catcacccat cggattggag atctggtgag cgaccggccc
     1021 atggagccgg ggcgcttcag tggcggccgg gcggtcgccg tggcaggcct catcgatgaa
     1081 gtgaagaagc gcgggccgcg ggtcatcctg cggctcgatg accgtaccgg tcggctcgag
     1141 gtcacgctct tcgaagacgt ttttcagcgc catcgcgatt tggtcgccaa ggatgcgctg
     1201 gtcttggtcg agggcatgct gcgcttcgat gagttcagtg attcctggcg gctctcggcc
     1261 cggcgcatca cggagctgga caaggtccgc gagcagcagg cccgccgtat cgtcctgaag
     1321 tggccgccgc acggcgacag cgcctcgctg ctgaggcggc tccgcgagat tctcgcgccg
     1381 tggcgcggcg gctcgtgtcc ggtgacagtc gaatacagcg gctcgggcgc gcgcggcgcc
     1441 ctcacgctgg ggcccgagtg gatggtcaag gccggccgcg agctgctcga gaagctcgaa
     1501 ggactcctcg gacgcggcgg cgtgcaagtt gtctacggca gtgcgccgag cgctgcgagc
     1561 acgcccaccg tgagcgccga tagcgggtaa gcctgtccgg cggccgcgac tgcggcttgc
     1621 aacgcgccgt tccggtttgc tcccgaggcg ccgctcccgt aacctcgccg cgtcagcact
     1681 ttccggtact gcgcatggcc gttgccttcc tggatttcga acaaccgatc gccgaactgg
     1741 aagcgaagat cgaggagctt cggcacgtca cctccgagag tgaggtcaac atcctcgatg
     1801 agatcaaccg cctgcagctg aagagccagc agctcacgag cagcatcttc gccaacctga
     1861 cgccgtggca gatcactcag ctcgcacgcc atccgcaccg cccttacacc ctggattacg
     1921 tgaattcgat cttcagcgag ttcaccgagc tgcatgggga tcgcatgttc gccgacgatc
     1981 tcgccatcgt gggcgggctc gcgcggctgg acgatcgtgc gctgatgatc atcggacatc
     2041 agaagggccg ggataccaag gagcgcgtgc gccgcaacta cggcatgccc aagccggagg
     2101 gctatcgcaa ggcgctgcgc ctgatgcgcc tcgccgagcg ctttggcctg cccctgctga
     2161 cgctcatcga tactcaggga gcctacccgg gcgtcggagc cgaggagcgc gggcagagtg
     2221 aggccattgc gcgcaatctc ttcgagatgt ccgtgctgag cgtgccgatc gttacggtgg
     2281 tcacgggcga gggcggatcg ggcggggcgc tggcgatcgg cgtgtgcgat cggctgctca
     2341 tgctccagta cagcacctat tcggtgatct cgcccgaagg ttgcgcctcc atcctctgga
     2401 agagtcagga caagaaggaa gcggccgccg aagcgctcaa tctgacggcg gatcggctct
     2461 tgaagctgag cctggtggac gaggtgatcg aggagccgct cggcggggct catcgcgatc
     2521 cgatcgagac cgcgcaccgg ctgaaggctg cgatcgtgcg ccacctcgat cagctcgaag
     2581 ccatgccgcg cgatgagctg cgcgcggcgc gctcggccaa gattgcctcc ttcggcctgt
     2641 tttccgagaa tccggaatag acactccgcc gcagaaattc gcacctcgct ggctggccag
     2701 gcgcctcgcg acactgctgc ccggctaccc cgatgtgcgg ctgtgcgtgg cgctcagcgg
     2761 cggtctggac tcgacggtgt tgctcgctgc gctggcgggc gcgcgccgaa gaagcgtgcg
     2821 gctgcgtgcc gtgcacgtga atcacggcct gcacccgaat gcagcgctct ggagcggaca
     2881 ttgtgagcga ctcgcctcgg cactcgatgt gccgctgacg atcctcaccg gacgtgtgat
     2941 gcgcggccgc ggtgtctcac tggaagccgc agcccgcgag acccgctacc ggctcctcgc
     3001 ggcaattctg acgcccggtg agttcctgct gagcgcgcac cacgaggatg accagctcga
     3061 gaccgtgatg ctgcagttgt ttcgcggcgc gggtctggcg ggcctcgcgg cgatgccggc
     3121 gctgaccgct ttcggcccgg gctggctggc gaggccgctg ctgccgtgca cccgtatgca
     3181 gctcgaggaa tgggggcgcc gctcggagct ctcctgggtc gaagacgata ccaacactga
     3241 tgagcgcctc gatcgaaact atctccgccg ccgcatcctg ccgctggtgc gcgatcgctg
     3301 gaagggggct gcttcagcgg tctcgcgtag cgcgcgccac gctgccgaag gccagcggct
     3361 gctcgatgct ctggcgcgca aggacgttga gcaggccgca ttcggaaccg agctctcggt
     3421 gagaagcctg cgcgcgctgt cggcggatcg gcgacgcaat gcgctgcgct tctggatcgc
     3481 gcactcggga cgaatgctgc ccgatacacg ccgtctggat gagctggccg gaccgatgat
     3541 cgatgctcgc ccggatgcca atccggtcgt gcgctgggcc gacacgctcg tacagcggca
     3601 taccgatctg ttgtggatcc ggccgagtcc caagccgctg ccgggcgaag gcgccggccg
     3661 gcgcaagctc cgcgctgagg ttcggggccg cccgatcgcg cgatccacgc actggtcatg
     3721 gcgagcctcc agtgccatcg acttgccgga caggtgcggc aagctcgagc tgaggccaga
     3781 tccgcacggc ccggtggatc tcgatgtgct gcccgagagc gtgagcatcc gctggcggga
     3841 aggcggcgag cggattcgcc cccggcgcgg cgggccccgt cgggccctca agagtctgct
     3901 gcaggaagcg cggttgcctc tggcgctgcg tgcgagcgtg cccctgatct tcgatgggga
     3961 tacgctgctc gccgtcggcg agctgtggct cgatgagtcc atccaggcga ccgccgccgc
     4021 accccatcgc gcccgcctcc tgtggagccc gcagagctga ggggcggtac ggggaagccg
     4081 cagcggcgag cgccgggggc tcaggtcgcg agcccgctca cgatgtggta acttttccgc
     4141 ccgtcgttca actcggcctt cacggtcctg gctcccgcga cggaaatccc ttcaagacat
     4201 atgacgcgtt tcgtattcgt caccggtggt gtcgtgtcct cgctgggcaa gggcatcgc
//
LOCUS       DASRHN010000395        14720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1790770, whole genome shotgun sequence.
ACCESSION   DASRHN010000395 DASRHN010000000
VERSION     DASRHN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14720
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790770"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFB37_15935"
     CDS             complement(<1..812)
                     /locus_tag="VFB37_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZO23989.1"
                     /translation="MLHEPNSTQGTADSPDEALDAYSRAVVGAVGSVGPAVVSVYVGG
                     ASEAARARGGAGSGVVVTPDGYLLTNEHVVQNVQDARVAFVDGRSASATVVGRDPSTD
                     LAVLRAQAASLPYARLTTTPPLRVGQLVVAVGNPFGFESTVSAGVVSALGRSLRSRHG
                     RLIEGVVQHTAALNPGNSGGPLVDSKGRVVGVNTAIIAMAQGIGFSVPAATAQWVLTE
                     VLTQGRVRRAYLGITVRDRPLDLRLVRALGLTVTRAVEITSRDEQGPAARSDL"
     gene            927..2699
                     /locus_tag="VFB37_15940"
     CDS             927..2699
                     /locus_tag="VFB37_15940"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HZO23990.1"
                     /translation="MISLSQTAVLLGAAVLAVSVFRLLRLSSILGYVAAGMAIGPWGF
                     GLVREVPSIMSFAEFGIVLLLFVIGLELQPTRLWVMRRIVFGMGFAQVCLCAALLGGA
                     AWLLGLPPPAALIVGFGLSLSSTPLVLQLLAERSQLKSTHGRAAFGILLFQDLAVLPV
                     LAALPLLSRSGTAASGGNGLLTLAKLLVVMAAIVGGGRLVLRPALRIVARLKVSEVFT
                     AASLLIVIATALLANEAGLSMSLGAFLAGVLLADSEFRHELEADLEPFKGLLLGLFFV
                     AVGMAADLGLLVSMPLRVLLVTVGFLLIKLLAVTALGRASGQAARSAWRLGFTLPAGG
                     EFAFVLFTLAAGERIVSQQTANLLILAVTLSMMLGPLLLILYDWLQRRSESEPQRPFD
                     GIDERENRVIIAGFGRFGQIVARVLQARHIAFTALDSNQTHVDFVRKFGNKVYYGDAS
                     RLDLLRAAGAENAAIFVVAIDDVEASVRAASLAREQFPRLKIFARARNRQHAFALKDI
                     GVRYIIRETFVSSLEMAAAVLETLGEPAASARAAVRKFRQHDEATLEAQYAVKEDETK
                     FLAASREAAQQLEGLFQADQTRSE"
     gene            2703..3350
                     /locus_tag="VFB37_15945"
     CDS             2703..3350
                     /locus_tag="VFB37_15945"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HZO23991.1"
                     /translation="MDEALRIREFWFSKAWTGPLPALGDAATRGQALARRAAIWFESG
                     SPARAQRDEQIRRAFTGLLERAAAGQLTSWADSPRRRLSLIILLDQFPRQIHRNTARA
                     FANDTQALGLTLSGMQSAADAALNVVERIFFYMPLQHAELPEVQDESVAAFARLRQEA
                     PTELSATFEASLESAQRHRELIQRFGRFPHRNAILGRDSTAEESAWLKDNPLAFG"
     gene            complement(3381..3704)
                     /locus_tag="VFB37_15950"
     CDS             complement(3381..3704)
                     /locus_tag="VFB37_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZO23992.1"
                     /translation="MSEPDKGSDTRVLGETDWIAASEVCQLCLIDLTVIVELVELGVV
                     ASRGRAPEEWLLPALSLPRLRVAGRLMRDLGVNVTGAALAIELLESQHRLERRIRHLE
                     RLAQE"
     gene            complement(3706..4689)
                     /locus_tag="VFB37_15955"
     CDS             complement(3706..4689)
                     /locus_tag="VFB37_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZO23993.1"
                     /translation="MEYRDYYQALGVSRSATADEIKKSYRRLARKYHPDVSKEPKAEE
                     KFKEVQEAYEVLKDPEKRAAYDQLGSEWKSGQNFRPPPDWGSGFEFRGGPSAGARQRS
                     AHQEEAFEDAAGFSDFFSSLFGARGFDGNGPFAASARPAARDHHARVDVDLEEAYSGT
                     TRTLELKRPELKPDGTLELKTHTVRVTIPAGVTEGQLIRLAGQGEQSSGQRKPGDLYL
                     EVRIKAHPLFQVDGRDITLTLPIAPWEAALGASVTVPTPGGAVEMHIPANSQSGQKLR
                     LRGRGLPGQPAGDEYVQLKVLVPPANSPEARALYEQMRQEMAFDPRAQLGR"
     gene            complement(4696..5583)
                     /locus_tag="VFB37_15960"
     CDS             complement(4696..5583)
                     /locus_tag="VFB37_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZO23994.1"
                     /translation="MPELPDITVYVEALEQRIGGARLESVRITHPFLLRSFEPSLEEL
                     QGRRVMSVKRVGKRVALQLEGDRWLVFHLMIAGRLHWFARGTAVPRKRALAYAVFESG
                     TLTLTEAGSKRRAALYVFASSEALAMHDPGGLEILESSYEQFRASLCRENHTLKRSLT
                     DPHTFSGIGNAYSDEILHRARLSPVALTQKLDEEGLRRLFEATRTVLSEWTQRLRDAA
                     DGEFPEKVTAFRPEMAVHGRFGKPCPVCATRVQRIRYAENETNYCPRCQTGGRILADR
                     SLSRLLKDDWPRTIEELEV"
     gene            complement(5722..7107)
                     /locus_tag="VFB37_15965"
     CDS             complement(5722..7107)
                     /locus_tag="VFB37_15965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZO23995.1"
                     /translation="MSAPTFGVKVPAHERIVLANGLTLILVPRRDVPLVAFNAVLRGG
                     ETADPAGKRGVASLVAGLLEKGAGARDAFGFADAVEGVGGIFNAGAGPESITVRGQFL
                     ARDQHLMLELLADVLLRPRLEPQELAYLRARQIELIKAAKDSDPAELIGIYGRAFLFG
                     DHPYGRPVSGSERSLGSIAHEDVLRHRLGQFGADRLTLVFAGDIDSEWLTRAVEQTFA
                     DWPRAQRTVSKLAQPARLGKRRVLLVDSPGSTQSYFWIGDVGVDRHYRERPALDLVNT
                     LFGGRFTSMLNTELRVKSGLSYGARSGFARGSVAGEFAIRSFVQTPDTSSALNLALRT
                     LGKLHDPEAITSAMLDSARAYVLGQYPLHFETAADWAAVFGEIELFELGSSYIDSYAP
                     QLCEVSVPQARRVIEEAFPSPEAVAMVLIGDAARLRESLLEYGPVTEMSLREPDYAAA
                     RAFAGEELAFT"
     gene            complement(7104..8435)
                     /locus_tag="VFB37_15970"
     CDS             complement(7104..8435)
                     /locus_tag="VFB37_15970"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZO23996.1"
                     /translation="MKKSFGSHPLHAGVLGTPAAAPLMPGRDLVRSRTLENGLRVIVW
                     PVHDIPNVALYNWVRAGSRNEAPGITGLAHFVEHMMFNGTTRHPPGEFDRQMEAQGGA
                     NNAFTSDDVTVYQDWCPRSALDLVLDLESDRIAHLAFVPEVIESERGVVASERRLRVD
                     DNNQGLLSEQVQASAFVAHAYRFPTIGWPSDIQAWRIEDLQSFFRTYYAPNNLTLILA
                     GDVTAADAFALVERHFGPIAPQAPPDRVRAREPEQVGERRVVLRRKAQTPLLQYAFKA
                     PAAADPRGPAVSLLLSVLVDGDSSRLHRTLVEQKRVAVEVGGHWHEGFDPGLLWLYLT
                     LPQGSDVEQTQSALDAELAKIAAQGVTDAELRRAKNMIAAAFWRKLATIDGKAQLLGE
                     YAVFHGDPLRLFDAPAQFEQVTRRQVQAVAREILDRRRRTVGVLVPEEEEA"
     gene            complement(8805..9557)
                     /locus_tag="VFB37_15975"
     CDS             complement(8805..9557)
                     /locus_tag="VFB37_15975"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZO23997.1"
                     /translation="MFTIRTKLAAIVVLALLIAGCSREQQDWRAAEAADTIEAYDQFV
                     QRHPESELVTEARTRVAQLGEERDWREAGAVDTADAYRQFLTQHPNGKWAQEARVRIE
                     SFALSGSPAPAPASGSGSASGSAALAESGSDAALASSAVASGISAARAGPGAAGAGSG
                     TPAPSAHGQNGAAAQLGAFNSEEAARREWRGLTGRFGPELRGLSPHIVAIDTASGRLY
                     RLQVLVSSEDRAHVLCDSLKKRGQGCMTVQTR"
     gene            9642..10043
                     /locus_tag="VFB37_15980"
     CDS             9642..10043
                     /locus_tag="VFB37_15980"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZO23998.1"
                     /translation="MSAPKRRLIDQLGSSPTFVAAGCHLSGDLEAAGAVIVSGSVRGD
                     GRVGGTLSMATGSQWDGEIHAQAAVIAGSISGRLVVVGKVEIRATAVVRADIVARNIA
                     IARGAIVDGEVTATGEEPIIRFDEKRKSGNA"
     gene            complement(10045..11070)
                     /locus_tag="VFB37_15985"
     CDS             complement(10045..11070)
                     /locus_tag="VFB37_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO23999.1"
                     /translation="MSLRPEQDFRPPRWLRNHHVQSMLASTIVRRGAVARRAAAVVEV
                     QKELLLDCGDGVRLQCFQSRPTHGNGRPVVILHGWEGSAQSLYILSLAQQLFERGFEV
                     MRLNLRDHGETHHLNRELFHSCRLADVVGALRALQALHPGEALRLAGFSLGGNFLLRA
                     AVLASEANLRIAQVVAVSPVLDPGETLSALEQSFPGYQLYFVRKWMRSLWKKQAVWPR
                     DYDFRELARMANLRRMTAELVRRFTEFPTLEDYLNGYAITGSRLARLEVPARIITSLD
                     DPIIPAHALQKLARASSLRLTVTRHGGHCGFLDRLSGPTWVERRIVAEFEAGRVEESH
                     AEILASA"
     gene            complement(11088..12452)
                     /locus_tag="VFB37_15990"
     CDS             complement(11088..12452)
                     /locus_tag="VFB37_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO24000.1"
                     /translation="MGAKRALIVDDSKSARLFLARMLEKYEIDVDSVENAESAIDYLA
                     THRPDVIFMDHLMPGMDGFQAVQAIKNNPVTATIPIMMYTSQEGELYLGQARALGAVG
                     VLPKQIKPTDVSKVLYQLHLLADRRSAYQASFRALTLQPPEGSAAEPSAANDSGLVPK
                     ILTETSLREQFAELRRALVAGVDTQTDRITAEVRALLLEAMPPPSPAQPADPLLPRST
                     WQWGWITAAIALAIALGTTALWWRQATQLEALTAEVTQLRHDIVTHGATTPTTSTSGA
                     ADLRAGGGALAPSAKTAAGQAPARALPTDFKPLVVTVPYGADAFGGPRVEIVRQLLDR
                     LVKEGRSGVVDIKSYAGRFCLMGNATDGFSLAPDETPFTRCDMVGNPAEEGLSAAQRI
                     PLPMANLIGGTRNASHGTLDVQPSTGDATLTVTPYPQVTPDLTAGAWNRAGSANNRIE
                     IRVR"
     gene            complement(12606..13781)
                     /locus_tag="VFB37_15995"
     CDS             complement(12606..13781)
                     /locus_tag="VFB37_15995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO24001.1"
                     /translation="MRVLLISDVYFPRVNGVSTSIRTFREDLAGCGIETLLVAPRYVS
                     SSAEECGVIRVRSSGVPGNPEDRRMQWGALVRALESVPRGRVDVVHIHTPFIAHYAGS
                     RFARRSKIPCVATYHTFFEEYLHHYVPVLPRRIGRFLARSFTRSQCDDVQALIAPSEP
                     MRDILLTYGISTPVHVVPTGLPADRFRAGDGARFRAAAGLPAERPLVSYIGRVAHEKN
                     IDFLVKVFVEVRRTVPQAMLVIAGEGPAREGLKSRIATLGLQQDVCFTGYLDRDTGLL
                     DCYAAADVFVFASRTETQGLVLLEAMAQGTPIVSTAELGTRSILKAASGALVVEERLE
                     PFAAAVVRLLQDPALRQDLAERGRNYARTWSSAAMARRIADLYESVQLPSSSAPAAA"
     gene            complement(13788..14327)
                     /locus_tag="VFB37_16000"
     CDS             complement(13788..14327)
                     /locus_tag="VFB37_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZO24002.1"
                     /translation="MKATGINQFIARVDAVEYGICRRLNRGASLLLPRVTFQLASRLG
                     DGIVWYVLILALPVLYGPAAVKPAMIMALTGVLGVALYKLLKRVFLRERPFITHSSIA
                     LAGAPLDRYSFPSGHTLHAVSFAWQASIHFPELTWVLVPLAALIASSRVALGLHYPTD
                     VLVGAAIGASLAELGISLT"
BASE COUNT         2502 a         4887 c         4859 g         2472 t
ORIGIN      
        1 aggtccgagc gcgcggccgg cccttgctca tcgcggctcg tgatctcgac cgcccgcgtc
       61 accgttaacc ccagagcgcg cacgagccgc agatccagcg gtcgatcgcg gaccgtgatg
      121 ccgaggtagg cgcgccggac acggccttgc gtgaggacct ccgtcaggac ccactgagcc
      181 gtggcggcgg gaaccgagaa accgatgccc tgcgccatgg cgatgatcgc ggtgttgacg
      241 cccacgacgc ggcccttcga gtccaccaga ggaccgccgg agttgcccgg gttcaatgcg
      301 gcagtgtgct gcactacgcc ctcgatgagt cgtccgtgac ggctacggag tgagcgcccg
      361 agcgcactca ccactccagc ggacacggtc gactcgaatc cgaagggatt cccgaccgcg
      421 acgaccagtt gtccgacacg aagcggcggc gtggttgtga gccgggcata gggaagcgag
      481 gcggcctgcg ctcgcagcac ggccagatcg gtcgaaggat cccgacccac gaccgtggcc
      541 gatgcgctgc gaccgtccac gaaggcgacg cgcgcatcct gcacgttctg caccacatgc
      601 tcgttggtaa ggaggtatcc gtccggggtg accaccacac ccgatccagc cccgcctcgc
      661 gccctcgccg cttcgctggc gccgcctaca tagacgctga cgacggccgg tccgacactc
      721 ccgacagccc cgacgacggc ccgtgaatag gcatccagtg cctcatcagg ggaatcggcg
      781 gtcccttgcg tagagttcgg ttcgtgcagc atggaagcgc aagggtgggg tctgcccggc
      841 cgaactcaag ccagaacgcc ccttgcccgg cacaacgacg cgatactatc tgccggctgc
      901 gcagctcgac gcatccggac cccatcatga tctcactctc ccagactgcc gttctgctcg
      961 gcgccgccgt gctcgccgtg tctgtcttcc gcctgctgcg gctgagctcc atcctgggtt
     1021 atgtggccgc cgggatggcg atcggcccct ggggcttcgg gctggtgcgc gaagtgccca
     1081 gcatcatgag tttcgcggag ttcggcatcg tgctgctgct gttcgtcatc ggcctggagc
     1141 tgcagcccac ccggctctgg gtgatgcgcc ggatcgtgtt cggcatggga ttcgcgcagg
     1201 tgtgcctgtg cgcagcgctg ctcgggggcg ccgcgtggct cctcggactg ccgccgccgg
     1261 ctgcactcat cgtcggcttc gggctctcgc tttcctccac accgctggtc ttgcagctgc
     1321 tcgcggagcg ctcgcagctc aagagcacgc atggccgtgc ggcgttcggc attctgctgt
     1381 ttcaggatct ggccgtgctg cccgtgctcg cggcgttgcc gttgctgtcg cgctccggga
     1441 cagcggcgag tggcggaaat gggctgctca cgctggcgaa gctgctcgtg gtcatggcgg
     1501 ccatcgtcgg cggagggcgg ctcgtactgc gtcccgccct gcggatcgtg gcgcgcctga
     1561 aggtgagcga agtgttcacc gccgcctcgc tgctgatcgt gattgcaacc gcgttgcttg
     1621 ccaatgaggc cgggctctcg atgtctctcg gcgcctttct cgccggagtg ctgctggccg
     1681 acagcgagtt ccggcatgag ctcgaggccg atctcgagcc cttcaagggc ctgctgctcg
     1741 gcctgttctt cgtggctgtc ggcatggcgg ccgacctcgg gctgctcgtc tcgatgcccc
     1801 tgcgggtgct cctggtcaca gtcggattcc tgctgatcaa gctgctcgcc gtcaccgcgc
     1861 tcggtcgcgc gagtggccaa gcggcgcgct ccgcctggcg gctagggttc acgctgcctg
     1921 ccggagggga gtttgcgttc gtgctgttca ccctggccgc aggggagcgc atcgtgagcc
     1981 agcagacggc caatctgctg atcctggccg tcacgctgtc gatgatgctc ggtccgctgc
     2041 tgctcatcct gtatgactgg ctgcagcgac gctcggagag tgagccgcag cgccccttcg
     2101 atggcatcga cgagcgggaa aaccgggtga tcatcgccgg tttcggcaga tttggtcaga
     2161 tcgtggcgcg cgtgctgcag gccagacaca tcgccttcac cgcgctggat tccaatcaga
     2221 cccacgtgga tttcgtgcgc aagttcggca acaaagtgta ttacggcgat gcctcccgtc
     2281 tcgacctgct tcgcgcggca ggcgcggaaa acgccgcgat cttcgtcgtg gccatcgatg
     2341 acgtggaggc ctcggtgcgt gcggcctccc tcgcgcgcga gcaattccca aggctgaaga
     2401 ttttcgctcg cgcccgcaat cgccagcacg cctttgcact caaggacatt ggcgtgcgtt
     2461 acatcattcg cgagacgttc gtttccagtc tcgaaatggc cgcggcagtg ctcgagactc
     2521 tgggggagcc ggcggccagc gcgcgcgctg cggtgcgcaa attccgccag catgatgagg
     2581 ccacgctcga agcgcagtat gccgtgaagg aggatgagac gaagttcctc gccgcctccc
     2641 gcgaagccgc gcaacagctc gagggcctgt tccaggcgga ccagacgcgc tcggaatgag
     2701 ggatggacga ggcgctcaga atacgcgagt tctggttcag caaggcctgg acagggccgc
     2761 tgcctgcgct cggcgatgcc gccacgcgcg gccaggcgct ggcgcgtcgc gcggccatct
     2821 ggttcgagtc gggctcgccc gcacgggcgc agcgggatga gcagatccgg cgcgcattca
     2881 ctggcctcct cgagcgcgcg gccgccgggc aactgaccag ctgggctgac agtccgcgca
     2941 ggcggctgag cctgatcatt ctactcgatc agtttccccg tcagatccac cgcaacacgg
     3001 ctcgagcgtt cgccaatgac acgcaggcgt tgggattgac tttgtcaggc atgcagtcgg
     3061 cggcggatgc cgcgctcaac gtggtggagc gtattttctt ctatatgccg ctgcagcatg
     3121 ccgaactgcc cgaagtgcag gacgaatccg tagcggcctt cgcacgcctg cggcaggaag
     3181 ccccgaccga gctgagcgcc acgttcgaag cgtcgctcga gtcggcccag cgccatcgcg
     3241 agctgatcca gcgattcggc cgatttcccc accgcaacgc catcctcggc cgcgacagta
     3301 cggcggagga gtccgcctgg ctcaaggaca atccgttggc gttcggctga gcagccgcat
     3361 gcggccgtca gcgtggccgc ttactcctga gccagacgct cgagatggcg gatgcgccgc
     3421 tccagccgat gttgggactc gagcagctcg atcgccaggg cggcgccggt cacattgacg
     3481 cccagatcgc gcatcagccg cccagcgact cggagccgcg gcagcgacag cgccggcaag
     3541 agccattcct ccggagcgcg cccacgcgag gcgaccaccc ccagctcgac gagctccacg
     3601 atcactgtca gatcgatcag gcacaactga cagacctcgc tggcggcgat ccagtccgtc
     3661 tcccccagca cccgggtatc cgatcctttg tcgggctctg acatgtcacc tcccgagctg
     3721 tgcgcgtgga tcgaaggcca tttcctgccg catctgctcg tacaacgcgc gagcctccgg
     3781 cgagttggcc ggcggcacga gcactttgag ctggacgtac tcatcgcctg cgggctgccc
     3841 cggcaggccg cgcccgcgca ggcgcagctt ctggcctgac tgcgagttcg cagggatgtg
     3901 catctcgacc gccccgcccg gtgttggaac cgtcacgctc gctccgagcg cggcctccca
     3961 cggtgcgatc ggcagggtga gggtgatatc gcggccatcg acctggaaca gggggtgagc
     4021 tttgatccgc acctcgagat acaggtcccc cggcttgcgc tgcccggagc tctgctcgcc
     4081 ctgtccggcg agccggatga gctgcccctc ggtgacgccg gccggaatgg tcacgcgtac
     4141 ggtgtgcgtc ttcagctcca acgtcccatc gggtttgagc tcggggcgtt tcagctccag
     4201 tgtccgagtg gtgcccgagt aggcttcctc gagatccaca tccacgcgtg cgtggtgatc
     4261 gcgcgctgcg ggccgcgccg atgcggcgaa aggtccgttg ccgtcaaatc cgcgagcgcc
     4321 gaacagcgaa gagaagaaat cgctgaagcc ggcggcatct tcgaaagcct cctcctgatg
     4381 tgcgctgcgc tggcgcgcgc cggcactcgg gccgccgcga aactcgaacc cacttcccca
     4441 gtccggcggc ggccggaagt tctgtccgga cttccactcg cttccgagct ggtcgtaggc
     4501 ggcgcgcttc tccggatcct tcaggacctc gtaagcctcc tgcacctcct tgaatttctc
     4561 ctccgccttc ggctccttgc tgacatccgg atgatatttg cgggccaggc gccgatagga
     4621 cttcttgatc tcgtccgcgg tcgcgctgcg actcacgccg agtgcctggt aatagtcgcg
     4681 gtattccatg agtaattata cctccagctc ctcgatcgtc cgcggccagt cgtccttcag
     4741 gaggcgcgac agagagcgat ccgccagaat ccggcccccg gtctgacagc gtgggcagta
     4801 gttggtctca ttctccgcat agcggatccg ctgcacgcgc gtcgcgcaca ccgggcaggg
     4861 ctttccaaag cgtccgtgca cggccatctc cggacggaac gcggtcacct tctcgggaaa
     4921 ctcgccgtcg gccgcgtcac gcaagcgctg cgtccactcg ctcagcacgg tccgggtcgc
     4981 ctcgaacagc cgacgcaggc cctcttcatc gagcttctgg gtcagagcca ccggcgagag
     5041 cctcgcgcga tgcaggatct cgtcggagta ggcgttgccg atcccgctga acgtatgcgg
     5101 atcggtgagt gagcgcttga gggtgtgatt ttcccggcac aaactcgccc ggaactgctc
     5161 atacgagctc tccagaatct ccaggccgcc cggatcatgc atcgccagcg cctcggagct
     5221 ggcaaacacg tacagcgcgg cgcgccgctt cgagccagcc tcggttagcg tcagcgtccc
     5281 ggattcgaac accgcgtagg cgagggctcg cttgcgggga accgccgtgc cgcgtgcaaa
     5341 ccagtgcagg cgaccggcaa tcatcaggtg gaagacgagc cagcggtcgc cttcgagctg
     5401 cagggcaact ctctttccaa cccgcttcac gctcatgaca cgccgcccct gcagctcttc
     5461 gaggctgggc tcgaagctgc gcagcagaaa gggatgggtg atccgcacgg actccaggcg
     5521 cgcgcccccg atacgctgtt ccagcgcttc gacatagacg gtgatgtctg gcagttcggg
     5581 catagcgagc gaaaatcaca cttttcgtga gcgggccgga agcgccgcag gcgtcttccg
     5641 gcaattatga caatagagcg aaaatcacac ttttcgtgag cgggccgcaa gcgccgcagg
     5701 cgtcttccgg caatacgaca gttacgtgaa tgccagctcc tcgcccgcaa aggcccgcgc
     5761 cgctgcgtag tccggctccc tcagcgacat ctccgtgacc ggtccatatt ccaacagact
     5821 ctcacgcagc cgcgctgcat cgccgatcaa caccatggcg actgcctcag gggacgggaa
     5881 ggcttcctcg atgacgcggc gcgcctgcgg cacgctcacc tcgcacagct ggggtgcata
     5941 gctgtcgatg taggagctgc cgagctcgaa cagctcgatc tcaccgaaca cggcggccca
     6001 gtccgctgcg gtctcgaaat gcagcgggta ctggccgagt acataggccc gggcggaatc
     6061 gagcattgca ctcgtgatcg cctccgggtc gtggagcttg cccagggtcc ggagcgccag
     6121 attcaaggcc gacgaggtgt ccggtgtctg cacgaatgag cggatggcga actcgcctgc
     6181 gacgctgccg cgcgcgaagc ccgagcgcgc gccgtacgac agaccggatt tcacgcgcag
     6241 ctcagtgttg agcattgatg tgaaacgacc cccgaacagg gtgttcacca gatccagcgc
     6301 ggggcgctcg cggtagtgac ggtcgactcc cacgtcaccg atccagaagt agctctgggt
     6361 ggaaccgggt gaatcgacca ggagcacgcg ccgcttgccc aggcgcgcgg gctgcgcaag
     6421 cttcgagacc gttcgctgcg cacgtggcca gtcggcgaat gtctgctcga cggcgcgggt
     6481 cagccactcg gaatcgatgt ccccggcgaa cacgagcgtg aggcgatccg ctccgaactg
     6541 gcccagtcga tggcgcagta cgtcctcgtg cgcgatgctg cccagcgagc gctcgctgcc
     6601 ggaaaccggg cgaccgtacg gatgatcgcc gaacaggaat gcgcggccgt agatgccgat
     6661 cagctcggcg gggtcggaat ccttcgcagc cttgatgagc tcgatctgcc gggcgcgcag
     6721 atacgccagc tcctgcggct cgagccgggg tcgcagcagc acgtcggcca gcagctcgag
     6781 catcaggtgc tggtcacgcg cgagaaactg accccggacg gtgatcgatt cgggcccggc
     6841 gccggcgttg aaaataccgc cgaccccttc cacggcgtcg gcaaagccga acgcatcacg
     6901 cgcgcccgca cccttctcca gcagcccggc caccagggaa gcgacacccc tctttccggc
     6961 aggatcggcc gtctcgccgc cgcgcaacac ggcgttgaaa gccacgagcg gcacgtctcg
     7021 ccgtggcacg agaatgagtg tcaggccgtt cgcgagcacg atgcgctcgt gggccggaac
     7081 tttcacgcca aaggtcggcg cgctcacgcc tcctcttcct ccggaacgag cacgccgacc
     7141 gtgcggcgtc gcctgtccag gatctctcgc gcgaccgcct gcacctgccg gcgcgtgacc
     7201 tgctcgaact gcgccggcgc atcgaagagt ctgagcgggt cgccgtggaa taccgcgtat
     7261 tcgcccagca attgggcctt gccgtcaatc gtggccagct tgcgccagaa ggctgcggcg
     7321 atcatgttct tggcgcgtcg cagctctgcg tccgtcactc cttgcgccgc gattttcgcc
     7381 agctcggcat cgagcgcgct ctgcgtctgt tcaacatcgg atccctgcgg cagtgtcagg
     7441 tagagccaca acagcccggg gtcgaagccc tcgtgccaat gcccaccgac ttccacggcc
     7501 acgcgcttct gctccaccag tgtccggtgc aagcgcgatg agtcgccatc gaccaacacc
     7561 gacagcagca ggctgacggc aggtccgcgc ggatcggcgg ccgccggtgc cttgaaggcg
     7621 tattggagga ggggtgtttg cgcctttcgc cgcaggacca cgcgacgctc gccgacctgt
     7681 tccggctcac gtgcccgaac cctgtccgga ggggcctgag gcgcgatcgg tccgaaatgc
     7741 cgttccacga gtgcaaacgc gtctgccgcg gttacatcgc ccgcgagaat gagtgtcagg
     7801 ttgttaggcg cgtagtaggt gcggaagaag ctctgcagat cctcgatgcg ccacgcctgg
     7861 atgtcggagg gccaaccgat cgtgggaaag cggtacgcgt gcgccacgaa ggcactcgcc
     7921 tgcacctgct cgctgagcag cccctggttg ttgtcatcca cgcgcagccg tctctcggag
     7981 gccacgactc cgcgctcgct ctcgatgacc tccggcacga aggcgaggtg ggcgatccga
     8041 tcggactcca gatcgaggac cagatcgagc gcactgcgcg ggcaccaatc ctggtacacc
     8101 gtgacatcat cgctcgtgaa ggcattgttg gcgccaccct gcgcctccat ctgccgatcg
     8161 aactcgcccg gcgggtgccg tgtcgtgccg ttgaacatca tgtgctcgac gaagtgcgcc
     8221 aggcctgtga tgcctggtgc ctcgttgcga ctgccggcac gcacccagtt gtagagggcc
     8281 acattcggga tgtcgtgaac cggccagacg atgacccgca atccattctc gagcgtgcgc
     8341 gaacgcacca ggtcgcgacc gggcatcagc ggcgcggccg ccggcgtgcc gagcacgccc
     8401 gcgtgcagag gatgcgaccc aaagctcttc ttcatggcct gcgcttctga cgatcgatcg
     8461 ggaaacaaag tcgtgaggaa gggataggtc ggcgacactt tcgtgccgca cggccgtacg
     8521 gtgacatagt gggtacggcc ggcatgggct acaaggtatg acccggccgc cgggttcccg
     8581 atcgggtgca gatagaaaaa agcgccgcgg gcgggggaag tcgcatcgcg gcgctgaagc
     8641 agcgaacacc ggggaagttg ttcgcaattc aaacttgcga tgagactgga gcaagagctg
     8701 tgccagtcag aaaataatag tttaatcagt ggcttgggag aaaaccgaga cgccgatcgc
     8761 ggtgacggga attgtcagct ttggaccgcc ggcagcgggt ttggtcagcg ggtttgcacc
     8821 gtcatgcagc cctgcccacg cttcttcagg gagtcgcata ggacgtgcgc gcgatcctca
     8881 ctactgacca ggacctgcag gcgatacaga cgtccggagg cggtatcgat cgcgacgatg
     8941 tggggcgaga gcccccggag ctcggggccg aagcgtccgg tgaggccccg ccattccctt
     9001 cgcgcggcct cttcgctgtt gaacgctccg agctgcgcgg cggcaccgtt ctggccgtgc
     9061 gctgaaggcg cgggggttcc cgagcctgcc ccggccgcgc ctgggcctgc cctggcggcc
     9121 gagatccctg aggctaccgc cgagctcgcc agggcagcgt cggaaccgct ctccgcgagc
     9181 gccgcggagc cactggccga gcctgagccg ctggcgggcg caggcgccgg tgagccgctc
     9241 agggcgaagc tttcgatgcg aacacgcgcc tcctgcgccc acttcccgtt gggatgctgc
     9301 gtcaggaact ggcgataggc atccgccgtg tccaccgcac ccgcctcgcg ccagtcccgc
     9361 tcctcaccga gctgtgccac gcgggtgcgc gcctcagtga cgagctcgct ctcgggatgg
     9421 cgctggacga actggtcgta cgcctcgatc gtgtcggccg cctcggctgc ccgccagtcc
     9481 tgctgctcgc ggctacagcc cgcgatgagc aacgcgagca ccacgatagc ggcgagtttt
     9541 gttcggattg tgaacatcgt cccaaccctc ttctgatgca ggtcccgacc cccgggctat
     9601 tctagtcccc ccctacggcc catccgaaga aggagtcgtc gatgagcgca ccgaagcgca
     9661 ggctgatcga tcagctcggt agctcaccga cgttcgtcgc cgccggctgt catctcagcg
     9721 gggatctcga agccgctggc gccgtgatcg tgagcggctc ggtccgtggc gatgggcggg
     9781 tcggcgggac actgagcatg gcaaccggtt cgcaatggga cggggagatc cacgcgcagg
     9841 cggcggtgat cgccggcagc atctccggca ggctggtggt cgtaggtaag gtcgagatcc
     9901 gggccaccgc cgtcgttcgt gccgacatcg tcgcccggaa tatcgccatc gcccgcgggg
     9961 cgatcgtcga cggcgaggtc actgcgaccg gcgaggagcc gatcatccga ttcgacgaga
    10021 aacggaaatc gggaaacgcc tgactcaggc gctggcgagg atctccgcgt gagactcttc
    10081 gacgcggccg gcctcgaatt ccgccacgat ccggcgctcc acccaggtcg gtccggacag
    10141 ccggtccagg aaaccgcagt gtcccccgtg ccgggtcacg gtcaaccgca gcgaggaggc
    10201 gcgtgccagc ttctgcagcg cgtgggcggg aatgatcgga tcatccagcg aagtgatgat
    10261 ccgcgcgggg acctcgagcc gcgccaatcg gctgccggtg atggcatagc cgttcagata
    10321 gtcctcgagt gtcggaaatt ccgtgaagcg acggaccagc tcggcggtca tgcgccgcag
    10381 atttgccatg cgcgcgagct cgcggaagtc gtagtcacgc ggccagacgg cctgtttctt
    10441 ccacagcgag cgcatccact tgcgcacgaa atagagctga tagccgggaa aagactgctc
    10501 gagggcgctg agcgtctcac cgggatcgag caccggcgag accgccacca cctgtgcgat
    10561 ccgcagattc gcctcggaag ccagaaccgc cgcacgcaac aggaaattac cgccgagcga
    10621 gaaccccgcc agcctgaggg cttcacccgg gtgaagcgcc tgcagtgccc gcagcgcacc
    10681 gaccacgtcg gcaaggcggc acgaatgaaa cagctcgcga ttcagatgat gagtttcgcc
    10741 gtgatcgcgc agattgagcc gcatgacctc aaaaccgcgc tcgaagagtt gctgcgcaag
    10801 tgacaggatg tacagcgact gcgcgctccc ttcccagccg tgcaggatga cgacgggtcg
    10861 gccattgccg tgcgtcggcc tggattgaaa acactgcagc cgaacgccgt cgccgcaatc
    10921 cagcagaagc tccttctgga cctccacgac agccgcagcc cgccgcgcaa cggcaccacg
    10981 gcgcacgatc gtgctcgcca gcatcgattg cacgtggtga ttgcgcagcc agcgcggcgg
    11041 acggaaatcc tgttcaggtc gaagcgacac ggcgaaccag cgctcgatca gcgcacccgg
    11101 atctcgatgc ggttgttggc actacccgcg cgattccagg ctccggcggt gagatcgggg
    11161 gtgacctgcg gatatggggt cacggtgagg gtcgcatccc cggtcgaggg ttgcacgtcg
    11221 agagtcccgt gcgaggcgtt gcgagtgccg cctatgaggt tggccatcgg caatggaatg
    11281 cgctgcgccg cggagaggcc ctcctcggcg gggttgccta ccatgtcgca ccgggtgaaa
    11341 ggggtttcat ccggcgccag agagaagcca tcggttgcat tgcccatcag acagaaacgt
    11401 cccgcatagg atttgatgtc gacgacgccg ctgcggcctt ccttgacgag cctgtcgagg
    11461 agctgtcgaa cgatctccac gcgcgggccg ccaaacgcgt ccgccccgta agggaccgtc
    11521 acgaccaggg gtttgaaatc cgtcggcagt gctctggccg gtgcctggcc ggccgcggtc
    11581 ttggcgctcg gggccagggc gccgccgccc gctcgcagat ctgctgcgcc gctcgtgctc
    11641 gtggtggggg tcgtagcgcc gtgggtcact atatcatggc gaagctgagt gacctccgcg
    11701 gtcagggcct cgagctgcgt cgcctgccgc caccacaagg cggtggtgcc aagcgcgatc
    11761 gccagggcga tcgcggccgt gatccagccc cactgccacg tcgatcgtgg cagcagcgga
    11821 tcggcgggct gagccggcga gggcggcggc atcgcctcga gcagcagcgc gcgaacctct
    11881 gcggtgatcc gatccgtctg ggtgtcgacc cccgcgacca atgcgcggcg cagctccgcg
    11941 aactgctccc gcaggctcgt ctcggtcaga atcttgggaa ccaggccgga gtcgttggcg
    12001 gcactcggct cggcagccga tccttcaggg ggctgcagcg tcagagcgcg gaagctggct
    12061 tgataggcgc tgcggcgatc ggccagcagg tgcagctggt acagcacctt cgagacatcc
    12121 gtcggcttga tctgcttggg cagcacgccg acggcaccga gcgcccgcgc ctgtccgagg
    12181 tacagctctc cttcctgcga ggtgtacatc atgatcggaa tggtggcggt caccgggttg
    12241 ttcttgatcg cctgcaccgc ctggaagcca tccatccccg gcatcagatg atccatgaag
    12301 atcacatcgg gacggtgcgt ggccagatag tcgatcgcgg attcggcgtt ctcgacgctg
    12361 tcgacgtcga tttcgtattt ttccagcatc cgtgccagaa acagccgcgc ggacttcgaa
    12421 tcgtccacga tcagcgcgcg cttggccccc atgccctgct ccaacacccc gcgaagactg
    12481 gcagtgtagc ggtgccgaaa gcgccccggg gcgaactttc agccgcgccg aaaagtgtag
    12541 cgcagaactc cggtcattcc agcgctgtcg cccgcgagcg acgaccgcgt cacgctacgg
    12601 tcggctcagg ccgcagccgg cgccgagctc gaggggagtt ggactgattc gtacaggtct
    12661 gcgattcggc gggccatggc ggccgaggac caggtgcgcg cgtaatttcg tccgcgttcc
    12721 gccagatcct ggcgcagggc cgggtcctgg agcaggcgca ccaccgcggc ggcgaacggt
    12781 tccagccgct cctccaccac gagcgcgccg ctggcggcct tgagaatgga ccgcgtgccg
    12841 agctctgcgg tcgagacgat gggagtgcct tgcgccatcg cctccagcag caccagacct
    12901 tgagtctccg tgcgggaagc gaatacgaag acgtccgcgg ccgcgtagca gtccagcagg
    12961 ccggtgtcgc gatcgagata gcctgtgaag cagacatcct gctgcaggcc cagcgtcgcg
    13021 atacgggact tcaagccctc tctggccggc ccctcacccg caatgaccag catggcctgc
    13081 ggcactgtgc gccgcacctc gacgaacacc ttcacgagaa agtcgatgtt cttctcgtgg
    13141 gctacgcgcc cgatgtagct gaccagcggg cgctcggcag gcagccccgc agccgcgcgg
    13201 aagcgagcgc cgtcacccgc tcgaaaacga tccgccggca aaccggtcgg cacgacgtgt
    13261 accggagtcg atatgccgta tgtcaggagg atgtctcgca tcggctccga tggtgcgatc
    13321 agcgcctgca cgtcatcgca ctgcgagcgc gtgaagctgc gcgccaagaa gcgtccgatg
    13381 cgccgcggca ggacgggcac gtagtggtgc aggtactcct cgaagaaggt gtgataggtc
    13441 gcgacgcagg ggattttcga gcggcgggcg aatcggctcc ccgcatagtg cgcaatgaac
    13501 ggcgtatgga tgtgcaccac atccaccctg cctctcggca cgctttcgag tgctcggacc
    13561 agcgcgcccc actgcatgcg acgatcctcc ggatttcctg gcacaccgga agacctcacg
    13621 cggatcacgc cacactcctc cgcagatgag ctcacgtacc gcggcgccac caggagagtc
    13681 tcgatgccgc aacccgccag atcctctcgg aaggtgcgga tggaggtgga aacgccgttg
    13741 actcgtggga agtagacgtc cgagatgagg agaacgcgca cgcggagtca ggtcagcgag
    13801 attcccagtt cggccaggga tgcaccgatc gccgcgccca cgagcacgtc agtggggtag
    13861 tggagcccga gcgccacgcg cgagctggca atgagcgctg caagcggcac gaggacccag
    13921 gtgagctccg ggaagtgaat cgaggcctgc caggcaaagg acactgcatg cagggtgtga
    13981 cccgagggaa agctgtagcg gtcgagcgga gcaccggcaa gcgcgatcga ggagtgcgtg
    14041 atgaacggcc gctcgcgcag gaaaacgcgc ttgagcagct tgtagagcgc cacacccagt
    14101 accccggtca gcgccatgat catcgccggc ttcacggccg ccggcccata cagaaccggc
    14161 agtgccagga tcagcacgta ccagacgatg ccgtctccca gacggctggc gagctggaat
    14221 gtgacgcgag gcaggaggag cgatgcgcct cggttcagcc tacggcaaat gccatattca
    14281 actgcatcca cgcgagcgat gaactggttg atacccgtcg ccttcatgtc agccttccgg
    14341 aacctcatgt tgcgggcgtc tggcttctgg cgcggtcagc ctcgctgcga tgcacgtcat
    14401 ctccgtgacg gaaatgtgaa ggtttctttg cgataggcgc ttgggaggta tttcgtgtgc
    14461 ggtgcctcac aatttgcgga ctcgtcgggc tttgaggcgc gtccagaagc aacttgacgt
    14521 ctgcgcgccg gcagcgagcg agtcgggggg caggtgggag cgaggcgcgc ggccggtgcg
    14581 agcgagtggg tatcaggtgc gagcgagtcg cgggggatag cgccgcagca aggcttcggt
    14641 caggcgctcg gcggagccgg cggcgtggga caggaacagc gaaggctcgg tgagctccag
    14701 ttccagcaga cagggggctc
//
LOCUS       DASRHN010000396         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1303737, whole genome shotgun sequence.
ACCESSION   DASRHN010000396 DASRHN010000000
VERSION     DASRHN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303737"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFB37_16005"
     CDS             <1..917
                     /locus_tag="VFB37_16005"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO24003.1"
                     /translation="LGEPLIAWSQGQRARPTPFAQRLVWAERRARIRMQPHIEALRCD
                     LARVLADARDERHQLLTVRASHDIALPILRQHAADAADLHIDIGFQGSAEALRALNER
                     QCVVAGFHVPILRGTAPVFARALKPLLKPKQHKLIACCRRTQGVMVRKEHAPLVRTFA
                     DTVLNGLRFVNRQPGSGTRLLVDHLLHEHSLSPGQLAGYADHVENTHVAVALCVASGV
                     ADAGIGIEAAALQFGLHFTPLVEENYFLACLEPSLEEPSVQRLREVLAGPRWREILGN
                     LPGYRGADAPGGLLLTDEALPWWESASR"
     gene            complement(963..2066)
                     /locus_tag="VFB37_16010"
     CDS             complement(963..2066)
                     /locus_tag="VFB37_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24004.1"
                     /translation="MIAELLTAIILLAFGTSHCMAAAQATAPAPKPAAAAPATATTQD
                     LTGTWQGKMKVDANTSLTIQFTFARKPDGSWSATLNSPDNPAIRNMAANAVSFTNGAV
                     KVDVSALNGSFSGTLKGRTLEGQWSQPGGALPLVMNPYEKPTIAKADLQTLLGGWHGP
                     LTVPGGTFTLVIRFKQNDKGELQGTMGVVEQGGGEAPVSDIEFANSELSFKLPGGRGD
                     YQGKYANGTFTGVLHPMGGPPNGMPVSLSKGDVAAPVYVLNLKTESFAALSGQWEGTL
                     EVTSPQGQKVTLPMVLNIGTNKNGEIVGSFDSPAQNVKGIPVTAATVEGSKVTFKCDP
                     LKAQYEADLANKTLTGQWTQGPVTAPLTLKKKS"
     gene            complement(2123..>4239)
                     /locus_tag="VFB37_16015"
     CDS             complement(2123..>4239)
                     /locus_tag="VFB37_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZO24005.1"
                     /translation="YYWEHSYFVDDNVFDVFTHKILYGDPKTALKDGTGTAVSATFAK
                     KYFGDRNPIGETITTDTGIPLKIALVFEDQPVNTHLKYDILFSSNVPFLRDQDNPTLR
                     RQALWNVGDMTYIVTAPGFDPSTWPRISDEFYKKYMADLGKQLKATWHSWLQPLTATH
                     LQTEVGYDLPNGNAVYLYGCAAVAVFILVVACINYMNLATARAARRARSVGIRKILGA
                     SRLRLGLQFLGEAVVFALIALILGVVLVELLLKFTPINSLLGQEVDLDLRRQPQLLAW
                     LLGLGVLMGLLSGLYPAIYLSSWAPLSALTGKTGAGRGNLRLREGLVLLQFTISAAVI
                     AATLLMGAQMRYIANKSLGFQKENRLMVTLRGTTTIDKIPTLRTELAKDSHILGVSET
                     QVMLGQDINIVMSDVDKDGGGTTSLTLKNEPIGEDFLQVMGLQLLQGRDLSKRLLTDV
                     GTNFLVNETLVRKLGWAEPLGKHITLGKGGGQSGRVIGVVRDFNFRSLHTEVEPLVMF
                     ATNHDFSNIPEVFRPFQQRLLIVNISGQDVGHTLGYIEKVMSQVDAKHPFEYQFLDET
                     LDKLYKSEHQLMGLIGIFSAVCIFIACLGLFGLASFTTEQRTREIGTRKVLGATTWQI
                     IALLARRILILVLIAAVLAAIAAYFAIDQWLAGFAYKTAINPLVFVLSAAAAAAIAFA
                     TVALQSFKTASADPVQALRFIE"
BASE COUNT          721 a         1310 c         1388 g          820 t
ORIGIN      
        1 cattgggcga gccgctcatc gcctggtcgc aggggcagcg ggcgcggccg acgccgtttg
       61 cgcaacggtt ggtctgggcc gagcgccggg ctcgcatccg gatgcagccg catatcgagg
      121 ctctgcgctg cgatctcgct cgcgtcctgg ccgatgcgcg ggatgagcgg caccagctgc
      181 tgactgtccg cgccagccat gacatcgcgc tccccatcct tcgccagcac gccgcggatg
      241 ccgcggatct gcacatcgat atcggctttc agggaagtgc cgaagcgctg cgggcgttga
      301 acgaacgaca gtgcgtcgtc gcgggcttcc acgttccgat cctgcgaggc acggctccgg
      361 ttttcgcccg ggcgctcaag ccgctgctca agccgaagca acacaagctg attgcgtgtt
      421 gccggcggac tcagggcgtg atggttcgca aggagcacgc gccgctggtg cggaccttcg
      481 cggacacagt gctgaatggc ctacgtttcg tgaatcgcca gccgggctcg ggaacgcgcc
      541 tgctggtgga ccatctgctc catgagcatt cgctgtcgcc ggggcagctt gcgggttacg
      601 ccgatcatgt cgaaaacacg cacgttgccg tcgcgctgtg cgtggccagc ggtgttgcgg
      661 acgccggcat cggaatcgag gccgcggcgc tgcagttcgg tctgcatttc acgcccctgg
      721 tggaggagaa ctatttcctg gcctgtctgg agccgagtct cgaggagccc tcggtccaga
      781 ggttgcgtga agtgttggcg gggccgcgtt ggcgggaaat actcgggaac ctgccaggct
      841 accggggggc ggacgctcca gggggattgc tgctcactga tgaagcgttg ccgtggtggg
      901 agtccgctag ccggtagatg tcctgagtat tcctgcccac gcgaccccaa tgcgcgtgac
      961 cctcagctct ttttcttcag ggtgagcggg gccgtcaccg gtccctgcgt ccattggccg
     1021 gtgagcgtct tgttcgccag atcggcctcg tactgcgcct tcagcggatc gcatttgaag
     1081 gtgaccttgc tcccttcgac cgtcgctgcg gtgaccggga tacccttgac gttctgcgcc
     1141 ggactgtcga acgagccgac gatctcgccg ttcttgttgg tgccgatgtt caacaccatg
     1201 ggaagcgtga ccttctgccc ctgaggactc gtgacctcca gcgtgccttc ccattgcccg
     1261 gacagggctg cgaacgattc ggttttcagg ttcaacacgt ataccggcgc cgcgacgtcg
     1321 cctttgctga gggacacggg catgccattc ggcggacctc ccatgggatg cagcactccg
     1381 gtgaacgtac cgttcgcgta cttgccttgg tagtcgcccc ggccgccggg gagcttgaag
     1441 ctcaattcgc tgttggcgaa ctcgatgtcg gaaaccggag cttccccgcc gccctgctcg
     1501 accacgccca tggtgccctg cagttcgcct ttatcgttct gcttgaaacg gatgaccaga
     1561 gtgaaggtgc cgcccggcac cgtcaggggc ccgtgccatc cgcccagcag tgtctggagg
     1621 tctgctttgg cgatggtcgg cttctcgtag ggattcatca ccaggggcag ggcgccgcca
     1681 ggttgcgacc actggccctc gagggtgcgt cccttcagtg ttccgctgaa cgacccattg
     1741 agtgcgctga catccacctt gaccgcgcca ttggtgaatg acaccgcgtt agcggccatg
     1801 ttcctgatgg caggattgtc cggcgagttc agtgtcgccg accacgagcc gtcgggcttg
     1861 cgtgcgaagg tgaactggat ggtgagcgac gtgttggcgt cgactttcat ctttccctgc
     1921 caggtgcccg tgaggtcctg ggtagtggcc gtggcaggcg cggcggccgc cggtttaggc
     1981 gcgggcgcag tcgcttgtgc cgccgccatg cagtgcgagg tgccgaaggc caacaggata
     2041 atggccgtca gcagctcggc gatcatcttg aatccactac acatgagaca gtccctccag
     2101 tggggtcgat tgttgaggtg tgtcattcga tgaaccgcag cgcctggacc gggtcggcgc
     2161 tggcggtctt gaaggattgc agcgccaccg tcgcgaaggc gatcgcggcg gccgccgccg
     2221 ccgacagcac gaagaccagc ggattgatgg ccgtcttgta ggcaaatccg gccagccact
     2281 gatcgatggc gaaataggcg gcgatggcgg ccagcaccgc ggcgatcagt acgagaatca
     2341 ggatgcgccg cgccagcagg gcgatgatct gccaggtcgt cgcgcccagc accttgcgcg
     2401 tgccgatctc gcgcgtgcgc tgctccgtag tgaatgaagc caggccgaac aggcccaggc
     2461 aggcgatgaa gatgcagacc gccgagaaga tcccgatgag ccccatgagc tggtgctcgg
     2521 atttgtacag cttgtcgagc gtctcatcga gaaactggta ctcgaacgga tgcttcgcat
     2581 cgacctggct catgaccttc tcgatgtagc ccagcgtatg accgacgtcc tgacccgaaa
     2641 tgttcacgat cagcaggcgc tgctggaagg gccggaagac ctccgggatg ttggagaagt
     2701 cgtgattcgt ggcgaacatg acgagcggct cgacctcggt atgcagcgag cggaaattga
     2761 aatcgcgcac gaccccgatc acccggccgc tttggccgcc gcctttgccc agggtgatgt
     2821 gtttgccgag cggctcagcc cagccgagct tgcgcaccag ggtctcgttg accaggaagt
     2881 tcgtgccgac atcggtcagc agccgtttgg acaggtcgcg gccctggagc agctgcagtc
     2941 ccatgacctg gaggaaatcc tcgccgatcg gctcgttctt cagggtgagc gaggtggtcc
     3001 cgccgccatc cttgtccaca tcggacatca cgatgttgat gtcctgtccc agcatcacct
     3061 gggtctccga gacgcccagg atgtgactgt ccttggcaag ctcggtgcgc agggtgggga
     3121 ttttgtcgat ggtcgtggtg ccccgcaggg tgaccatcag gcgattctct ttctggaacc
     3181 cgagggactt gttggcgatg tagcgcatct gcgcgcccat cagcagcgtg gcggcgatca
     3241 cagcggccga gatcgtgaat tgcagcagca cgagtccttc gcgcaggcgc aggttccccc
     3301 gccccgcgcc ggtcttgccc gtcagcgccg acagcggggc ccacgaagac aggtagatcg
     3361 ccggatagag accggacagc agccccatca gcacgcccag gcccagcagc caggccagca
     3421 gctgcggttg cctgcgcagg tcgagatcga cttcctgccc cagcagcgaa ttgatcggcg
     3481 tgaatttcag cagcagctcg actagcacga cgccgagaat cagtgcgatc agcgcaaaca
     3541 cgaccgcttc gccgaggaac tgcaaaccga gccgcagccg gctggcgccc agaatcttgc
     3601 ggatacccac cgaacgcgcc cggcgcgcag ctctggcggt cgcgaggttc atgtagttga
     3661 tgcaggccac caccaggatg aacaccgcca cggcggcgca cccgtacagg tagaccgcat
     3721 tgccgttggg caggtcgtac ccgacttcgg tctgcagatg cgtggcggtc aagggctgca
     3781 gccagctgtg ccacgtggcc ttgagctgct tgcccagatc cgccatgtac ttcttgtaga
     3841 actcgtcgct gatgcgcggc cacgtcgacg gatcaaaacc cggagccgtc acgatgtacg
     3901 tcatgtcgcc gacattccag agcgcttgcc tgcgcagcgt cgggttgtcc tgatcgcgca
     3961 ggaacggcac gttgctggag aagaggatgt cgtacttgag gtgagtgttg accggctgat
     4021 cctcaaagac gagggcgatc ttgagcggga tgcctgtgtc ggtggtgatc gtctccccga
     4081 tcggattcct gtcgccgaag tacttcttcg cgaacgtggc gctgaccgca gtgcccgtgc
     4141 cgtccttcag cgcggttttg ggatcgccgt acagaatctt atgggtgaac acgtcgaaga
     4201 cgttgtcgtc gacgaaatag ctgtgctccc agtagtaag
//
LOCUS       DASRHN010000397         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1469120, whole genome shotgun sequence.
ACCESSION   DASRHN010000397 DASRHN010000000
VERSION     DASRHN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469120"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFB37_16020"
     CDS             <1..703
                     /locus_tag="VFB37_16020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO24006.1"
                     /translation="LIGQSASTFTLPAAAANLNPPVVPSGLPSELLERRPDIAANERR
                     MAAANAQIGIARAAYFPAITLSGAGGFESTSIRSWFNGPSGFFSVGVAAVETLFDAGR
                     RRAISDQAKAAFNESAANYQQSVLGAFQEVEDNLAALRLLEGEAKTQEAAVASAQRSL
                     ELSNNRYKGGVATYLEVITAQSTALSDQRVAVQIAGRRMTAAVGLIKALGGGWSTAEL
                     PMAQRDSRPQANNGQ"
     gene            783..2429
                     /locus_tag="VFB37_16025"
     CDS             783..2429
                     /locus_tag="VFB37_16025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZO24007.1"
                     /translation="MRPRSEASRELESQVFDVCVIGGGATGAGCALDAQLRGFKTALV
                     EAGDFGSATSSTSTKLAHGGVRYLQQAVTQFDFAQLHIVRQALRERKRMLENAPHLAR
                     CCDFLVPCFSRWDCWYYGLGLRIYDGLAGPAALGRSRRLSRRETLVRMPELRGDALHG
                     SVVYQDGQFDDARYCLALVKSFSGAAGAATNYVKVVGFEKGADGKLAAALARDAFSGA
                     QTTIRARAFVNATGPFSDSLRSLANPSAEPRLILSKGAHILLPLEREQPALLIPRTED
                     GRVIFALPWMGRLLLGTTDERVAHPDGVSVTRQDAEYLLSHLNRYCRREYALSEIVGA
                     FAGVRPLLGSAHGRSTKDLVREHEIEIDSTSGLISILGGKWTSYRVMAEGAIDRVQEK
                     LGKPGACRTRDYRLAGGEAFGTDLWQEVARDAGLPEATARRITNTLGTEAKAILSLIG
                     EHPEWRTPLTPDASRIQAEVIHCARSEMAVTVEDVLARRLGLQFIDWELAMQAAAPTA
                     ELLGAELGWPIEQRKRAAQEFVARVQQQITTVRAEAAANG"
BASE COUNT          443 a          814 c          828 g          451 t
ORIGIN      
        1 gctgatcggg cagtccgcct ccaccttcac gctgcccgcg gcggcggcga atctcaaccc
       61 cccggtggtt ccgtccgggc ttccgtccga gttgctggaa cgccgccccg atatcgccgc
      121 gaacgagcgc cgcatggcag ccgccaacgc acagatcggc attgcgcggg ccgcatactt
      181 tccggcgatt actctgagcg gggccggagg ttttgaaagc acctcaatcc gtagctggtt
      241 caacgggccc agcggctttt tctcggtggg cgtcgccgcg gtggagactc tatttgacgc
      301 cggacggcgg cgcgccatca gcgaccaggc caaggccgca ttcaacgaat ctgcggccaa
      361 ttaccagcag tccgtcctgg gagcgttcca ggaagtggag gacaatctgg cggccttgcg
      421 cctgcttgag ggcgaggcca agacccagga agcggccgtc gcctccgcgc agcgctcgct
      481 cgagctgtcc aacaaccgct acaaaggcgg agttgcaaca tacctggaag tgatcaccgc
      541 gcagagcacc gctctcagcg accagcgtgt cgccgtgcag attgcaggac ggcgcatgac
      601 cgcggcggtg gggctgatca aggcgcttgg cggaggatgg agcactgccg agcttcccat
      661 ggcgcagaga gattcccgcc cgcaagcgaa caacggtcag tagggggcga gcccccgggc
      721 tgcccgcgtc tgccatcccc ggatcctcgc cgtcgggccg cgctacactg aacctgctca
      781 tcatgagacc ccgctcggaa gcctcgcgcg aactcgagtc gcaagtcttc gatgtgtgtg
      841 tgattggggg cggagcgacc ggcgcgggtt gcgcgctcga cgcgcagctt cgcggattca
      901 aaacggccct tgtggaggct ggtgatttcg gctcggcgac gtcgagcacc tcgacgaagc
      961 tggcccacgg cggcgtccgc tacctgcagc aagcagtgac gcaattcgat tttgcgcaac
     1021 tccacatcgt gcggcaggcg ctgcgcgagc ggaaacgcat gctggagaac gctccccatc
     1081 ttgcccgttg ctgcgacttc cttgttcctt gtttcagccg ttgggattgc tggtactacg
     1141 gcctgggcct gagaatctac gacgggcttg cgggcccggc tgcgctcggg cggagccgcc
     1201 ggctctcccg gcgtgagacg ctggtccgga tgccggagtt gcgcggcgat gcgctgcatg
     1261 ggtcggttgt ctaccaggac ggtcaattcg acgatgcgcg ctactgcctc gcgctggtca
     1321 aaagtttcag cggcgctgcc ggcgcggcga ccaactacgt gaaggtggtt ggtttcgaga
     1381 agggcgctga cggcaagctt gccgccgctc tcgcccggga cgcgttcagc ggagcgcaga
     1441 ccacgattcg cgcacgggca tttgtcaatg ccaccgggcc tttctcggat tcgttgcgct
     1501 cgctcgctaa cccctcggcg gagccccggc tcattctgag caaaggcgct cacatcctgc
     1561 tgccactcga gcgtgagcaa cccgccctgc tgattccgag gaccgaagac ggccgtgtga
     1621 tcttcgcgct tccctggatg gggaggctgc tgctcgggac taccgacgag cgcgtggctc
     1681 atccggacgg tgtctcggtt acccggcaag atgccgagta tcttctctcg cacttgaatc
     1741 gctactgcag aagggaatat gcgctcagcg agattgtggg cgcctttgcc ggagtccgtc
     1801 cgctgcttgg ttcggcacac ggccggagca cgaaggactt ggttcgcgag cacgaaattg
     1861 agattgattc caccagtggg ctcatcagca ttctgggtgg caagtggact tcctatcggg
     1921 tcatggccga aggcgccatc gatcgcgtgc aggaaaaact gggcaagcct ggcgcctgcc
     1981 gcacccggga ctatcggctc gcgggcggcg aggcttttgg gacggatctg tggcaggaag
     2041 ttgcgcgcga cgcagggctg cccgaggcaa cggcacggcg catcacgaac acgctgggca
     2101 ccgaagcgaa ggccatcctt tccctgattg gcgaacatcc cgaatggcga acgccgctaa
     2161 cgcccgatgc ttcccggatc caggccgagg tgatccactg cgcccgttcc gaaatggctg
     2221 tgaccgtgga agatgttctc gcacggcgcc tcggattgca gttcatcgat tgggagctgg
     2281 ccatgcaggc ggcggccccg actgccgaac ttctcggtgc cgaactcggc tggcccatcg
     2341 agcagcggaa acgcgcagcc caggaattcg tggccagagt acagcaacag atcaccaccg
     2401 tgcgcgcaga ggcagccgcc aatggctaaa tatctcggtg ccatcgacca ggggacaacc
     2461 agcacccgct tcatcgtgtt tgacccttcc ggacgcatgc tcgcctgtga gcagcaggaa
     2521 caccggcaga tctgcc
//
LOCUS       DASRHN010000398        22500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1793512, whole genome shotgun sequence.
ACCESSION   DASRHN010000398 DASRHN010000000
VERSION     DASRHN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22500
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793512"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2899
                     /gene="carB"
                     /locus_tag="VFB37_16030"
     CDS             <1..2899
                     /gene="carB"
                     /locus_tag="VFB37_16030"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZO24008.1"
                     /translation="YEVELIAASRAAIDMAEDRELFRNAMREIGLETPKSQIARSLED
                     ALEIVGEIGYPTVIRPSFTLGGSGGGIAYNREELEEIVARGLDASPTEEVLLEESVIG
                     WKEYEMEVVRDHKDNCIIICSIENVDAMGVHTGDSITVAPALTLTDKEYQRMRDASIA
                     VLRKIGVDTGGSNVQFAVNPRDGRLLVIEMNPRVSRSSALASKATGFPIAKIAAKLAV
                     GYTLDELRNDITGGTTPASFEPSIDYVVTKIPRFTFEKFPAANDRLTTQMKSVGEVMA
                     IGRTFQESLQKALRGLETGLDGLNEQLAQPLTDELQERLAYELRAPGANRLLYTADAF
                     RAGWSLERIAELTHIDPWFLAQLEDLILEEARVREDGLGALTGDRLRTLKRKGFSDSR
                     LAKLVDMNEKAIRSKRHELGVLPVYKRVDTCAAEFATSTAYLYSTYEEECEASPTTRR
                     KIMILGGGPNRIGQGIEFDYCCVHAALQLREDGFETIMVNCNPETVSTDYDTSDRLYF
                     EPLTLEDVLEVIAKEKPEGLIVQFGGQTPLKLSRALEEAGAPIIGTTPDSIDIAEDRE
                     RFQALVRKLGLKQPANATARTEDQAVRLAREVGFPLVVRPSYVLGGRAMEVVFTEEDL
                     RAYMTHAMKVSNDSPVLLDRFLDVAIEVDVDAVCDGEDVLIGGIMEHVEQAGVHSGDS
                     GCSLPPNSLGPEAQAELREQTRKLARGLNVVGLMNVQFAIQNGVIHVLEVNPRASRTV
                     PFVSKATGLPLAKIAARVMTGRKLKDLGILHEPAPAYYSVKEAVFPFVKFPEADPILG
                     PEMKSTGEVMGTGRTFGEAYAKAQAASGVILPRNGVCFISVRDRDKAGAVMLARKLVE
                     RGFELLATNGTARALNEAGIACRRVNKVREGRPHIVDMIKNDEVDLIVNTTEGKQAIR
                     ESNSIRREAVHKRVTYYTTLAAGLATCEALDHMDEAEVNRLQDLHQEALRA"
     gene            2896..3372
                     /gene="greA"
                     /locus_tag="VFB37_16035"
     CDS             2896..3372
                     /gene="greA"
                     /locus_tag="VFB37_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZO24009.1"
                     /translation="MRRTPMTLRGAEALRAELRKLKSEARPNIIKAIAEARGHGDLSE
                     NAEYHAAREQQGFIEGRISEIESKLGNAEIIDPSKLPNSGKVVFGAVVELDDQDDANR
                     LVYQIVGEDEADIRAGRISITSPIARALVGKSEGEVVDVTAPGGVRSYEIVAVRFE"
     gene            complement(3397..3780)
                     /gene="yhbY"
                     /locus_tag="VFB37_16040"
     CDS             complement(3397..3780)
                     /gene="yhbY"
                     /locus_tag="VFB37_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HZO24010.1"
                     /translation="MNTRTPRPGSSSTPAKGTRTAGRAPILTERQRRYLRGLAHSLKP
                     VIRLGSAGLTAGVTGETERALHDHELIKVKAAGADHETRDATFGDLAQQTASALVQRI
                     GNVAVLYRPRADMPRILIPAGDPQA"
     gene            3906..4025
                     /locus_tag="VFB37_16045"
                     /pseudo
     CDS             3906..4025
                     /locus_tag="VFB37_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            4096..4356
                     /locus_tag="VFB37_16050"
     CDS             4096..4356
                     /locus_tag="VFB37_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZO24011.1"
                     /translation="MAPNLSGLDVIDPPRALYLAELALDTAGRLLKPGGAALIKMFQG
                     AGFQELVRSARAGVAQVRLSKPQASRARSPEMYLLAKDFLMV"
     gene            4402..6321
                     /gene="ftsH"
                     /locus_tag="VFB37_16055"
     CDS             4402..6321
                     /gene="ftsH"
                     /locus_tag="VFB37_16055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZO24012.1"
                     /translation="MNDLTKNILLWVVIVLVLLAVFSRYMPTVGQPEEVRYSTFLDDV
                     KANRVDSVVLQGEMIYGVRKDKTQFKTFNPETDYTALIGTLQKANVAIEGRPPKQPNF
                     LAQLLLQLAPALLLILVFVYMLRQMQGASGGRGAMSFGKSRARLLGEDQVNVTFADVA
                     GVDEAKQEVSEIVDFLKDPGKFQKLGGKIPKGVLMVGSPGTGKTLLARAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQ
                     ILRVHMRRVPLADDVKPGLIARGTPGFSGADLANLVNEAALFAARANKRIVGMEEFER
                     AKDKIMMGAERRSMVMSEQEKRMTAYHEAGHAIVGMTVPEHDPVYKVTIIPRGRALGV
                     TQFLPEQDRYSLSKRRLESAIATLFGGRIAEELIFGPESVTTGASNDIERATELARNM
                     VTKWGLSDKLGPLTYSEETGEVFLGRSVTQHKQVSDGTAHAIDEEVRRVIEGNFQRAR
                     QILVGALDKLHSMADALIKYETIDEEQLKDIMAGKTPKPPKDWDETLSNKPPKAPPAE
                     GAPGGGPIGSPAGQH"
     gene            6410..7249
                     /gene="folP"
                     /locus_tag="VFB37_16060"
     CDS             6410..7249
                     /gene="folP"
                     /locus_tag="VFB37_16060"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZO24013.1"
                     /translation="MATLLVRCGTRTLDLSRPVVMGVLNVTPDSSSDGGRYSSVAAAV
                     EHGMRMAEEGAALIDVGGESTRPGAEAISLEEELRRVLPVIESLCAACPAIISIDTSK
                     PAVMRAAARAGAGLINDVRALSQDGALETAVASGCAICLMHMKGEPSNMQRDPTYADV
                     VREVGEFLRARAARCLAAGMTSDRLLVDPGFGFGKNLEHNVQLLRHLRELGSQWPVVV
                     GLSRKSMAGRLTGRPAGQRLYGSLALAVIAVMNGARIVRAHDVAPTVDVIRTAGAVME
                     AEG"
     gene            7246..8607
                     /gene="glmM"
                     /locus_tag="VFB37_16065"
     CDS             7246..8607
                     /gene="glmM"
                     /locus_tag="VFB37_16065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZO24014.1"
                     /translation="MSLGRKYFGTDGVRGRVGEHPMTVDFALLLASAAARVLAPEGGT
                     VLIGKDTRVSGYMFESALEAGFVAAGVNAMLIGPLPTPGIAYMTRRFNCDFGVVISAS
                     HNPYDDNGIKFFDASGAKLSDELEERIEAELLQAPVTRTSRSLGRATRVDRSRTHYQD
                     FCASTIPPGLDLSGLKIVVDCANGAAYKVGPRVLADLGAEIVPIGCSPNGRNINDGCG
                     SMAPDLLQLTVPGVRAHVGLALDGDGDRLVMVDHLGRIVDGDQLLYVIARARREAGTL
                     SAPVVGTVMSNLGLEIALRQSGIEFRRAPVGDRYVLALLRETGGTLGGETSGHILCLD
                     KTTTGDGVVSALQVLAVMKQTGRSLAELASGMDKFPQVLLNVKVAKRFDPASVPAVQA
                     AVQRIEQRMAGHGRVVLRASGTEPVIRVMVEGKNEATTRAAASELAEVVRVAAEQEPC
                     SAE"
     gene            8674..9426
                     /gene="tpiA"
                     /locus_tag="VFB37_16070"
     CDS             8674..9426
                     /gene="tpiA"
                     /locus_tag="VFB37_16070"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZO24015.1"
                     /translation="MRRPIVVGNWKMHGSRAENARLIEDLASQYPRSTAADCVVCPPL
                     VYLFEVGRMLRETTIGLGAQDVCAESHGAFTGEVSAAMLKDVGCEYAIVGHSERRSIY
                     HESDQLIARKFTAAQSRGLVTILCIGEQLAEREQGQTAEVVSRQLDIVLEICSVQALH
                     QAMIAYEPVWAIGTGRNASPAQAQEVHALIRARISARDAKIGAAIRILYGGSVKASNA
                     AELFAMPDVDGGLIGGASLKADEFLAILAAAA"
     gene            9444..9839
                     /gene="secG"
                     /locus_tag="VFB37_16075"
     CDS             9444..9839
                     /gene="secG"
                     /locus_tag="VFB37_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZO24016.1"
                     /translation="MLRIILTVVQVFAAFAIIGLVLIQRGKGAEAGAGFGAGASGTVF
                     GARGASTALSRATAIFAAIFMLNSLALAYMGTKQAAEPKSILDEAGESAQKSSSGAAK
                     SKPALPGPASIQTPPPPAPQGSPGTPPAK"
     gene            9895..9979
                     /locus_tag="VFB37_16080"
     tRNA            9895..9979
                     /locus_tag="VFB37_16080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9929..9931,aa:Leu,seq:gag)
     gene            10115..10483
                     /locus_tag="VFB37_16085"
     CDS             10115..10483
                     /locus_tag="VFB37_16085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZO24017.1"
                     /translation="MLSNYLPILIFLVLATALAVVLLVLGTGLGRYFARFHQDPAKRS
                     PYECGFDAFEDSRMKFDVRYYLVAILFIVFDLEIAFLFPWAVALSKIGGFGLIAMAIF
                     LGILVVGFIYEWKKGALEWD"
     gene            10545..11009
                     /locus_tag="VFB37_16090"
                     /pseudo
     CDS             10545..11009
                     /locus_tag="VFB37_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            11010..11726
                     /locus_tag="VFB37_16095"
     CDS             11010..11726
                     /locus_tag="VFB37_16095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZO24018.1"
                     /translation="MEPSKNIEALALQVDAHLKEGVRRVPALPDELAYEAEPAALLSV
                     CRTLRDAPELKFEMLMDLAGVDYLHYGRDDWQTTTATHSGFSRARVMRTQPPDPEMAG
                     RFGVTYQLLSITHNRRMRLRVKCPDTQEPVIDSVIDLWAAANWFEREAFDLYGILFRG
                     HPDLRRILTDYGFIGHPFRKDFPLIGNVEVLYDTEKRRVVYQPVSIVPRVLVPKVIRH
                     DPRYEPALRDVVPEELRGPR"
     gene            11726..12979
                     /locus_tag="VFB37_16100"
     CDS             11726..12979
                     /locus_tag="VFB37_16100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZO24019.1"
                     /translation="MSEIRNYTLNFGPQHPAAHGVLRLVLEMDGETIQKADPHIGLLH
                     RATEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVLAIERLLGITPPERARYIRVMFD
                     EITRILNHLMWLGAHGLDIGAMTVFLLCFKEREELMDVYEAVSGTRMHATYYRPGGVY
                     RDLPDTMPKYQVSRWRSQKEVDRLNEARAGSMLDFIAAFTDRFPARIDDYETLLTDNR
                     IWKQRTVNIGVVSPERALQLGFSGPMLRGSGVEWDLRKKQPYEVYDRMNFDIPVGVHG
                     DCYDRYLVRIEEMRQSNRIVRQCVDWLSANSGPVMIDDRKVRPPRRERMKDDMESLIH
                     HFKFFTEGYEVPEGETYAAIEAPKGEFGIYLISDGANKPYRLKCRAPGFPHLAALEEM
                     SLGHMLADVVAIIGTQDIVFGEIDR"
     gene            12976..13506
                     /locus_tag="VFB37_16105"
     CDS             12976..13506
                     /locus_tag="VFB37_16105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZO24020.1"
                     /translation="MSSEINSGEKAPVTGKVRLLSQHTRAEIDLWVRKFPPGRQRSAC
                     LSALRAAQEQNKGYLTTDLMDAVAEYLGLPPIQVYEVATFYSMFELHPCGRHHVSICT
                     NISCMLNGAEELVAHAERKLGIKLNQSTPDGRIFLKQEEECLAACTGAPMMMVDHIFH
                     EDLTPEKVDAILDELK"
     gene            13503..14822
                     /gene="nuoF"
                     /locus_tag="VFB37_16110"
     CDS             13503..14822
                     /gene="nuoF"
                     /locus_tag="VFB37_16110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZO24021.1"
                     /translation="MSADYSDRMNLVVFEPLKLERSWTLETYRKIGGYEVWERLLSEK
                     PPREQIIDAVKASGLRGRGGAAFPTGMKWSFMPRNSPVQKYLVCNSDESEPGTCHDRD
                     ILRFNPHSLIEGLAIAGYATNTTVAYNYIRGEFLGEPIPRFEAALAEAYAAGLLGKDV
                     RGSGIDFDIHTFVGAGAYICGEETAMLDSLEGKPGKPRFKPPFPANFGLYGAPTTINN
                     TQSFASVPTILRRGPEWFVGLGPKNSAGTVIFSVSGHVAKPGNFELPLGIAFADLLQI
                     AGGMRGGRALKAVIPGGASVPVLPAEIILKTNMDYDSLKAAGSAIGSAAVMVMDETTC
                     MVRVLERISRFFMSESCGQCTPCREGTGWMNRLIRRIMAGDARREELGLLLDVANRIE
                     GHTICALGDASAWPVQSFLKHYKHEFEYMIDHGGRSMVSEQLGARAA"
     gene            14819..16996
                     /gene="nuoG"
                     /locus_tag="VFB37_16115"
     CDS             14819..16996
                     /gene="nuoG"
                     /locus_tag="VFB37_16115"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZO24022.1"
                     /translation="MSAEEFVNLEVDGVPLKARKGEMLIRVTDAHNIYVPRFCYHDKL
                     SIAANCRMCLVEVEKAPKPMPACATPVVEGMKVFTRSPRAIGAQRATMEFLLINHPLD
                     CPICDQGGECELQDLAMGFGRDLSRFHERKRSVPDENLGPLIATDMTRCIHCTRCIRF
                     TQEIAGYQELGMVGRGEQMKVRTYVEQTVHHELSGNVIDLCPVGALVSKPYRFSARAW
                     EMISHPLVAPHDPVGSNLFGHVLRGKLMRVIPRENEAINETWISDRDRFSYEGVYSPE
                     RIEQPMLRRGSGPDAEWVETSWEDALAKVVEGLRPRAAELGVLASSTSTVEELYLLGR
                     LARGLGTPHIDHRLRQRDCRDQEADPAFPGLGMRIEAVDSLSALLVVGADLRREVPIL
                     AHRVRKAALRGAKIAFLGPAKSEYLFPVAATVVSSPAQQVTELAAVLSVVAEATSRTV
                     PAHLSDIVRGASVADTHRAIGTALLSGERRALWLGALALRHPAYADLRALAAAIAELA
                     GASLGVLAEGGNAAGAYLAGAVPHREAGGRPASRTGMAALEMLQHPLKAYLLFGGVEP
                     WIDSASADSAQSLARSEFVVAMTPFANNQLRAVAHVLLPIGTFAETSGTYVNMEGLWQ
                     SQTGAALPVGASRPGWKVLRVLGNLLALEGFDYQSSEEVREELRALCAEPDATQYRGS
                     HRVAQQPGSAEILDLNMYQVDALVRRAPSLQKTREGRTLPVTY"
     gene            17015..18085
                     /gene="nuoH"
                     /locus_tag="VFB37_16120"
     CDS             17015..18085
                     /gene="nuoH"
                     /locus_tag="VFB37_16120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZO24023.1"
                     /translation="MLQTLSNAWASLPDYGRTTVWTVVIMIVLIICVALLTLWERKVI
                     GWMQLRRGPNRVRIFGLAPGLGQPFADVLKLLMKEVIIPADANKILFRLAPVIALVPA
                     FAAWAVIPLSPEVVVSKANAGLLYLLSLTSMGVYGVIIAGWAANSKYAFLGAMRSAAQ
                     MVAYEIAMGFALVGVIIAAQSLNLGDIVERQQGGFLAWNWFWLFPLMMVYFISGVAET
                     NRAPFDVAEGESEIVAGFHVEYSGIAFALFFLAEYANMILISALTAVFFFGGWLSPFA
                     GYLGDSWLARPSFLWLGLKIFCALFLFLWFRATFPRYRYDQIMRLGWKVLIPVTIVWI
                     GVEMLLAWLRVGPWAHQALWAQ"
     gene            18100..18582
                     /gene="nuoI"
                     /locus_tag="VFB37_16125"
     CDS             18100..18582
                     /gene="nuoI"
                     /locus_tag="VFB37_16125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZO24024.1"
                     /translation="MAFNPIKTLLLTELIQGLWVTRRTFFSRKYTLNYPEEKTPQSPR
                     FRGLHALRRYPNGQERCIACKLCEAVCPAVCITIDSEVAPDGTRRTSRYDIDLFKCIY
                     CGFCEEACPVDAIVLTRIHEYHMEHRGENIMSKDKLLAVGERYEAMIATDKAADAPYR
                     "
     gene            18593..19246
                     /locus_tag="VFB37_16130"
     CDS             18593..19246
                     /locus_tag="VFB37_16130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZO24025.1"
                     /translation="MLVEVLFYVFGTILVAGALAVIISRNPVYSALSLVLCFVTSAAI
                     WLLIEAEFLAVILVLVYVGAVMVLFLFVVMMLDIDLEELRTGFTRFAWLGWLTALAVI
                     VEIVGVVWVRGLGIDVTRSPTPTAANYSNTLALGRVLYTKYAYPFELAAVLLLVAIIA
                     AISLTMRKRPGLKAQNIAKQVAVNSKDRVRLVKMEAEKPAEGAADLGQPAQAEQKAQ"
     gene            19243..19548
                     /gene="nuoK"
                     /locus_tag="VFB37_16135"
     CDS             19243..19548
                     /gene="nuoK"
                     /locus_tag="VFB37_16135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZO24026.1"
                     /translation="MITLAQLLTLSSILFALAVAGIFLNRKNVILLLMCVELLLLAAN
                     FNFIAFSRYLGDINGQVFVFFVLTVAAAESAIGLAILVVLFRERRSINVEDLNKLRG"
     gene            19556..21625
                     /gene="nuoL"
                     /locus_tag="VFB37_16140"
     CDS             19556..21625
                     /gene="nuoL"
                     /locus_tag="VFB37_16140"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZO24027.1"
                     /translation="MNPDILIAIAVLPLLGALIAGLFGRVIGRAGAHTVTIAGVAVSC
                     ALSLYILKQSYWDGVATFSGPVYTWLVSDGVHLEVGFLIDRLTALMMAVVTFVSLCVH
                     VYTIGYMHDDDGYTRFFSYISLFTFSMLMLVMSNNFMQLFFGWEAVGVVSYLLIGFWY
                     TRPSAIFASLKAFLVNRIGDFGFVLGIAGIFYFTNSLAYADAFAAAPQIAHATLPLSS
                     NTSVEALTLICICLFVGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMV
                     ARMSPLFERSDTALSFVMVIGATTAFFMGLLGIVNNDIKRVIAYSTLSQLGYMTAALG
                     ASAYSAAIFHLMTHAFFKALLFLAAGSVIIALHHEQDMRKMGGLAKYMPITAVTSWIG
                     ALALIGTPFFSGFYSKDSIIEAIGEAHRRGATYAYYCALGGVFITALYTFRMIFLTFH
                     GPERFRSPEAVHSCASDQSIGHEETEMSPAAHGEAASTHAASDHGSHEPPHESPLVVT
                     LPLIALAIPSLLIGIFTIGPVLFGDYFGSSIQVLQQNDVVRELAREFHGPVAFALSGF
                     TKPPFWLAAAGVFTAWLCFLHRPQLADQAARALSPLRTLLINKYYFDWFNERVLATLA
                     RGVGISLWKAGDQVLIDGAVVNGSAATVRWFGSVVRRVQNGYLYAYAFWMVIGLAVLL
                     GWFLEHS"
     gene            21644..>22500
                     /locus_tag="VFB37_16145"
     CDS             21644..>22500
                     /locus_tag="VFB37_16145"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZO24028.1"
                     /translation="MHGSLSVLIWLPIAAGIAVLLLGERNIAAGRWVALIASIVTLLV
                     CLPLLSHFDTSTAAPQFTEHVLWISRFHAYYALGLDGISLPLVVLTAFVTIPVIIAAW
                     TVIEARPAQYFASFLIMEGLMIGVFSATDALLFYFFWEAMLIPMFLIIGIWGGPRRVY
                     ATIKFFLYTFLGSVFMLVALIYMYVKAGDYSIASFQGLPLTLIEQRWIFVAFLLAFAV
                     KVPMWPVHTWLPDAHVEAPTGGSVILAAIMLKMGGYGFLRFSLPIAPDASRELDLLLI
                     SLSLIAVVY"
BASE COUNT         3830 a         7087 c         7410 g         4173 t
ORIGIN      
        1 gtatgaggtc gagttgattg ccgcctcgcg ggcggcgatc gacatggccg aagaccgcga
       61 gctgttccgt aacgcgatgc gcgagatcgg gctcgagacg ccgaagtcac agatcgcccg
      121 cagtctggag gatgcgctcg agatcgtggg tgagatcggc tatccgacgg tgatccggcc
      181 ctccttcaca ctcggcggct ccggaggcgg catcgcctat aaccgcgagg agctcgagga
      241 gatcgtggcg cgcggactcg atgcctcgcc gaccgaggaa gtgctgctcg aggagtcggt
      301 gatcggttgg aaagagtacg aaatggaagt cgtacgcgac cacaaggaca actgcatcat
      361 catctgctcc atcgagaacg tcgatgcgat gggcgtgcac acgggcgact cgatcacggt
      421 cgcgccggct ctcacgctca ccgacaagga ataccagcgc atgcgcgatg cctccatcgc
      481 cgtgctgcgc aagatcggcg tggataccgg cggctccaac gtgcagtttg ccgtgaatcc
      541 gcgcgatggc cggttgttgg tcatcgagat gaacccgcgc gtctcgcgct cgtcggcgct
      601 ggcctcgaag gcgaccggct ttccgatcgc caagatcgca gcgaagctcg cggtcggtta
      661 caccctcgat gagctcagga acgacatcac gggcgggacg acccccgcct ccttcgagcc
      721 gtcgatcgac tacgtggtca cgaagatccc gcgtttcacc ttcgagaaat tcccggcggc
      781 caacgatcgg ctgacgacgc agatgaagtc ggtgggcgag gtgatggcga tcgggcgtac
      841 cttccaggaa tcgctgcaga aggcgctgcg cggactggag accgggctcg acgggctgaa
      901 cgagcagctt gcccagccgc tcaccgatga gttgcaggag aggctcgcgt atgagctgcg
      961 ggcgccgggc gcgaaccggc tgctgtacac cgcggatgcc tttcgggcgg gctggtcgct
     1021 cgagcgcatc gcggagctga cacacatcga tccgtggttc ctggcgcagc tcgaggatct
     1081 gatcctcgag gaggcacggg tccgcgagga tggactcggc gcactgacgg gcgatcgcct
     1141 gcgcacactc aagcgcaaag gcttctccga cagccgcctg gcgaagctcg ttgacatgaa
     1201 cgagaaggcc atccgctcca agcgtcacga gctgggcgta ctgccggtct acaagcgggt
     1261 ggatacctgt gcggctgagt tcgccacctc gacggcatat ctgtattcca cctacgagga
     1321 ggagtgcgag gcgagcccca ccacgcggcg caagatcatg atcctcggcg gcggtccgaa
     1381 ccggatcggt cagggaatcg agttcgacta ctgctgcgtg cacgcggcgc tgcagctgcg
     1441 cgaagacggc ttcgagacca tcatggtcaa ctgcaacccg gagaccgtct cgaccgacta
     1501 cgatacctcg gaccggctgt acttcgagcc cctcacactc gaggatgtcc tcgaggtgat
     1561 cgccaaggag aagccggagg ggctcatcgt tcagttcggc ggtcagactc ccttgaagct
     1621 gtcccgcgcc ctggaggagg caggagcgcc gatcatcggc acgactcctg actcgatcga
     1681 catcgccgag gaccgcgagc gcttccaggc cctggtgcgc aaactgggtt tgaagcagcc
     1741 ggcgaacgcg acagcgcgta ctgaggacca ggccgtgcgg ctggcgcgcg aggtcggttt
     1801 tccgctcgtc gtccgccctt cctacgttct gggcggccga gcgatggagg tcgtcttcac
     1861 cgaggaggac ctgcgagcct atatgacgca cgccatgaag gtatccaacg acagtccagt
     1921 gttgctcgat cgcttcctgg atgtcgcgat cgaggtggac gtggatgccg tgtgcgacgg
     1981 agaggacgtg ctgatcggcg gaatcatgga gcacgtcgag caggccgggg ttcactcggg
     2041 cgactccggc tgttctctgc cccccaacag cctcggcccg gaagcccagg cggagctgcg
     2101 cgagcagacc cgcaagctcg cccggggcct gaacgtcgtg ggactgatga acgtgcagtt
     2161 tgccatccag aatggcgtga tccatgtgct cgaggtgaat ccgcgggcct cgcgtaccgt
     2221 gccgttcgtt tccaaggcga ccgggctgcc cctggccaag attgcggccc gggtgatgac
     2281 cggcaggaag ctcaaggacc tcggaatact ccatgagcct gcaccggcct attactccgt
     2341 gaaggaggcg gtgtttccct tcgtcaagtt ccccgaggcc gacccgatcc tcgggccgga
     2401 gatgaaatcc accggcgagg tcatgggaac cgggcgcacc ttcggagagg cctacgccaa
     2461 ggcgcaggca gcctcggggg tgatcctgcc gcgcaacggg gtatgcttca tcagcgtgcg
     2521 cgaccgggac aaggccgggg cggtgatgct cgcccgcaag ctcgtcgagc gtggcttcga
     2581 gctgctggcg accaatggaa ccgcacgggc gctgaatgaa gcggggatcg cgtgccgccg
     2641 cgtcaacaaa gtacgagagg ggcggccaca catcgtggac atgatcaaga acgacgaggt
     2701 cgatctcatc gtcaacacga ccgagggcaa gcaggccatt cgcgaatcca actccatccg
     2761 tcgcgaggcg gtccacaagc gggtgacgta ctacacgacg ctggctgccg ggcttgcgac
     2821 ctgtgaggcg ctcgaccaca tggacgaagc cgaggtcaac cgcctgcagg atctgcatca
     2881 agaggcactg cgcgcatgag acgcacaccc atgacgctgc gtggcgctga ggcgttgcgt
     2941 gcggaactga ggaagctcaa gtccgaagca cggccgaaca tcatcaaggc gatcgcggag
     3001 gcgcgcggtc acggtgacct gtcggagaac gccgaatatc atgctgcgcg cgagcagcag
     3061 ggcttcatcg agggccgcat cagcgagatc gagtcgaagc tcggcaacgc cgaaatcatc
     3121 gatccgagca agcttcccaa cagcggcaaa gtggttttcg gcgcggtggt ggagctggac
     3181 gatcaggacg atgccaaccg gctcgtgtat cagatagtgg gcgaggacga ggcggacatc
     3241 cgcgccggca gaatctccat cacctcaccg atcgcgcgag ctctggtcgg gaagtccgag
     3301 ggtgaagtgg tggatgtgac cgcccccggc ggcgtgcgct cctacgagat cgtcgcagtc
     3361 agattcgagt gaggcgcgcg acctgcgctt gagcgctcag gcctgcgggt cgcccgcggg
     3421 aatcaggatc cggggcatat cggcgcgggg tcgatagagc acggcgacgt tgccgatgcg
     3481 ctgtacgagc gcgctggcgg tctgctgggc cagatcgccg aaggtcgcat cccgggtctc
     3541 gtggtctgcc ccggcagcct tcaccttgat gagctcgtga tcgtgcaagg cacgctcggt
     3601 ctcgcccgtg acgccggccg tcagcccggc gctgcccagg cggatgaccg gcttgaggct
     3661 gtgcgcaaga ccgcggaggt acctgcgctg ccgctcggtg aggatcgggg cacggccggc
     3721 agtccgagtt cccttggccg gagtggatga ggagccgggt cggggcgttc gggtgttcat
     3781 ggcaccattg tataggccac agcggggctg gaacttcaca gatgggcagg cgtttcgaaa
     3841 agcagtgggc ggtggctcga ggagcactgc gcggatccgt tcgtgcagcg ggcccaggct
     3901 cagggatggc gctcacgcgc ggttttcaag cgcggggagt tgacctgaag gagaagctgc
     3961 tgaaaccggg acagtgcagc ctggatctgg gagcggcgcc cggtgcctgg agccagtacg
     4021 cccgccgccg ggtgggccgc tccggtcgcg tggtggctac cgacatactc ccgatcgatc
     4081 ccctccccgg cggacatggc ccccaatctc agcggcttgg atgtcatcga cccgccgcgt
     4141 gccctgtatc ttgccgagct tgcactcgat accgccggtc ggctcttgaa gcctggcgga
     4201 gctgccctga tcaagatgtt ccaaggcgct ggatttcagg agctggtgcg ctcggcgcgc
     4261 gctggtgttg cccaggtcag gctgagcaag ccccaggctt cgcgcgcgcg gagccccgag
     4321 atgtatttgc tggcgaagga ctttctgatg gtgtaggttg ttttttgcag cctgcaccgt
     4381 gatccaaaga ggtcggaccc tttgaacgat ttgacaaaaa acatccttct ctgggtggtc
     4441 atcgtgctcg tgctgctggc ggttttcagc cgctacatgc cgacggtggg ccagccggag
     4501 gaggtgcggt actccacgtt cctcgatgac gtgaaggcca accgggtgga ctcggtcgtg
     4561 ctgcagggcg agatgatcta cggggtgcgc aaggacaaga cccaattcaa gaccttcaac
     4621 ccggagaccg attacaccgc cctcatcggg acgctgcaga aggccaacgt ggccatcgag
     4681 ggccggccgc cgaagcaacc caatttcctg gcgcagctgc tcttgcaact cgcacctgcc
     4741 ctgctgctga tcctggtatt cgtctacatg ctcaggcaga tgcagggcgc ctccggaggc
     4801 cgcggggcga tgtccttcgg caagagccgt gcgcggctgc tgggcgagga tcaggtcaac
     4861 gtcacgtttg ccgatgtcgc gggcgtggac gaagccaagc aggaagtgtc ggagatcgtt
     4921 gacttcctga aggaccccgg caagttccag aaactcgggg gcaagatccc gaagggtgtg
     4981 ctgatggtag gctcacccgg gaccggcaag acgctgcttg cgcgcgccat cgcgggcgag
     5041 gcgaaggtgc cgttcttcac catctcgggc tcggatttcg tcgagatgtt cgtcggtgtc
     5101 ggcgcctcgc gggtgcgcga catgttcgag caggcgaaga agcacgcccc ctgcatcatt
     5161 ttcatcgacg agatcgatgc cgtcgggcgc caccgcggtg cgggactcgg cggcggtcac
     5221 gatgagcgcg agcagaccct gaatcagctg ctcgtggaaa tggatggctt cgaaggtaac
     5281 gagggcatca ttgtcatcgc cgcgaccaat cgtcccgatg tgctcgatcc ggcgctcctg
     5341 cgcccgggcc gattcgatcg gcaggtggtt gtaccgctgc ccgacgtgcg cggccgcgag
     5401 cagattctgc gcgtgcacat gcgccgcgtg cccctcgccg atgatgtgaa gccggggctg
     5461 atcgcgcgcg gcacacccgg attctccggc gccgacctgg ccaatctggt caacgaggcg
     5521 gcgctgttcg ccgctcgggc caacaagcgc atcgtgggaa tggaagagtt cgagcgcgcc
     5581 aaggacaaga tcatgatggg cgccgagcgc cgctccatgg tcatgagcga gcaggagaaa
     5641 cgcatgaccg cctaccatga ggccggccat gcgatcgtgg gcatgacggt tccggagcac
     5701 gacccggtct acaaggtgac gatcatcccg cgcggtcgcg cgctcggcgt cacacagttc
     5761 ctgcccgagc aggaccgcta tagcctgtcc aagcgccggc tcgagagtgc gatcgcgacg
     5821 ctgttcggcg gtcgcatcgc cgaagagctg atcttcgggc ccgaatcggt caccacgggg
     5881 gcctccaatg acatcgagcg tgcgacggag ctggcgcgca acatggtcac caagtggggg
     5941 ctgtcggata agctgggtcc gctcacctat tcggaggaga ccggcgaggt ctttctcggc
     6001 cgcagtgtca cgcagcacaa gcaggtctcg gacggcaccg cgcacgcgat cgacgaggaa
     6061 gtgcggcgtg tgatcgaagg caacttccag cgcgccagac agatcctggt cggcgctctc
     6121 gacaagctgc attccatggc ggacgcgctc atcaagtacg agacgatcga cgaggagcag
     6181 ctgaaggaca tcatggccgg caagacgccc aagccgccca aggactggga cgagacgctg
     6241 tccaacaagc ctcccaaggc gcccccggcg gaaggcgcac ccggcggcgg acccatcggc
     6301 tcccccgctg gccagcactg attcaataga aacatcgcct gcggcgattt ctcggagctc
     6361 agcttcgatg accggtgccg gagccgccgc gccgcgttga ccggagtgag tggcaacctt
     6421 gttggtgcgg tgtggaacga ggacgctgga tctgtcacgc ccggtggtca tgggtgtgct
     6481 caatgtcacc cccgattcct cctccgatgg cggccgttac tcaagcgttg cagccgcggt
     6541 ggaacacggc atgcgcatgg ccgaggaggg ggcggccctc atcgatgtgg gcggggagtc
     6601 cacccggccg ggcgccgaag cgatctctct ggaggaagag ctgcggcggg ttctgcccgt
     6661 gatcgagagc ctctgtgcgg cctgcccagc catcatctcg atcgatacca gcaaaccggc
     6721 cgtgatgcga gctgcggcgc gcgctggagc cgggctgatc aacgacgtac gagccctctc
     6781 gcaggacgga gcactcgaga ccgctgtggc gagcggctgc gccatctgcc tgatgcacat
     6841 gaaaggcgag cccagcaaca tgcagcggga cccgacctac gcggatgttg tgagggaggt
     6901 cggcgagttt ctgcgggcgc gcgccgccag atgcctggcg gccggcatga catccgatcg
     6961 cctcctggtc gatccgggct tcgggttcgg caagaacctg gagcacaatg tgcagctgct
     7021 gcgtcacctg cgcgagctgg gatcgcaatg gcctgtcgtg gtcggcctgt cgcgcaagtc
     7081 catggccggt cggctgacgg gtcgtcccgc gggccaacgg ctctacggca gcctggcact
     7141 tgcggtgatt gcagtgatga atggcgcgcg tatcgtgcgt gcgcacgatg tggctccgac
     7201 cgtggatgta atcagaacgg ccggcgcggt aatggaggcg gaggggtgag tttgggacgc
     7261 aagtacttcg ggacggacgg cgttcgcggc agggtcggtg agcatccgat gaccgtggat
     7321 tttgcgctgc tgctggccag cgctgctgcg cgtgtgctcg cccccgaagg cggtacggtc
     7381 ctgataggca aggacacgcg cgtttcaggc tacatgttcg aatcggcact ggaagcgggc
     7441 ttcgtcgcgg ctggcgtcaa cgcgatgctg atcggacctc tgcccacgcc aggcatcgcc
     7501 tacatgacac gtcgcttcaa ctgcgatttc ggcgtggtca tcagtgcttc gcataatccg
     7561 tacgacgaca acggaatcaa gttcttcgat gcctcgggcg cgaagctctc ggacgagctc
     7621 gaggagcgca tcgaggcgga gctgttgcag gcaccggtga cgcgcacctc gcgcagtctc
     7681 ggccgcgcta cgcgcgtcga tcgctcgcgg acgcactatc aggatttctg cgcctccacc
     7741 attccgccgg gcctggattt gtccgggctg aagatcgtcg tggactgcgc gaatggggcc
     7801 gcctacaagg tcggccctcg cgtgctggca gatctcggag ccgagatcgt gccgatcggc
     7861 tgctctccga acggtcgcaa catcaacgat ggctgcggct ccatggcgcc ggacctgttg
     7921 cagctgacag tgcccggcgt gcgcgcccac gtgggtctgg cgctcgacgg tgacggcgac
     7981 cgcctcgtca tggtcgatca cctcgggcgg atcgtggacg gcgaccagct cctgtatgtc
     8041 attgcccgcg cgcgccggga ggccggcaca ttgagcgcgc cggtcgtcgg cacggtcatg
     8101 agcaatctgg gcctggagat cgcattgcgc cagagcggca tcgagtttcg gcgtgcaccg
     8161 gtcggcgatc gttacgtgct cgcattgctg cgcgagaccg gtggcacgct cggcggggaa
     8221 acctcaggtc acatcctatg cctcgacaag accaccactg gagatggtgt cgtgagcgcc
     8281 ctgcaggtgc tcgccgtgat gaagcagacg ggacgctcgc ttgccgagct cgcctccggc
     8341 atggacaagt ttccccaggt gctgctcaac gtgaaggtcg ccaagcgctt cgaccccgcc
     8401 agcgttccgg cggtacaggc agcagtgcag cggatcgagc aacgcatggc aggccacggc
     8461 cgggtcgtgc tgcgggcttc gggtaccgag ccggtcatcc gggtcatggt cgaaggcaag
     8521 aatgaagcca cgacacgcgc cgcggcctca gagctcgccg aggtcgttcg tgtggcggcg
     8581 gagcaggagc cctgcagcgc ggaatagtgg cgctgcaacg cacggcgagt tttggtttat
     8641 gatccgcgcc cccctcgggc cagaggccgc tccatgcgcc gccccatcgt cgttggaaac
     8701 tggaagatgc acggctcgcg tgccgagaac gcgcggctga tcgaggatct tgcgagccag
     8761 tatcctcgct ccacggccgc cgactgcgtg gtgtgccccc cgctcgtata cctgttcgag
     8821 gtcgggcgga tgttgcgcga aacgacgatc ggtctgggtg cccaggacgt gtgtgccgaa
     8881 agccatggcg cattcactgg cgaggtctcc gccgccatgc tcaaggacgt gggctgcgag
     8941 tacgccatcg tgggacattc ggagcgacgc tcgatctacc acgaaagcga ccaactgatc
     9001 gcgcgcaagt tcacggcggc ccagtcgcgg ggtctggtta ccattctgtg catcggcgag
     9061 cagctcgccg agcgcgagca gggacagacg gccgaggtcg tttcccgcca gctcgatatc
     9121 gtgctggaga tctgctccgt gcaggcgctg caccaggcga tgattgccta cgagccggtt
     9181 tgggccatcg gcacgggccg caacgcatca cccgcgcagg cgcaggaggt gcatgccctg
     9241 atccgggccc gaatctcggc ccgggatgct aaaatcggtg ccgcgatacg cattctttac
     9301 ggcggcagcg tcaaggcgag caatgccgcc gagctgttcg cgatgccgga cgtggatggg
     9361 gggttgatcg gcggcgcctc gctcaaggcc gatgagttcc tggcgattct ggcggctgct
     9421 gcgtaattga cgatggattt cctatgttac ggatcatatt gacagtggtt caggtgttcg
     9481 ctgcgttcgc catcattggg ctcgtgctca ttcagcgcgg caagggagcg gaggcgggcg
     9541 cgggcttcgg cgccggcgca tcggggacgg tcttcggcgc gcgcggcgcg agcacggccc
     9601 tgtcgcgggc aacggccatc ttcgccgcta ttttcatgct gaacagcctg gcgctggcct
     9661 acatgggaac caagcaggcg gcggagccca agtcgatcct cgatgaagcc ggcgagtctg
     9721 cgcagaagag cagctctggc gcggcgaaga gtaagccggc gttgccgggc cccgcgtcga
     9781 tccagacacc tccgccgccc gcgccgcaag gttcgccggg tacaccgccg gcgaagtagg
     9841 tgcggcagga agaagcggga tctttgtctt cccggcgcga gccggtcatg gcgagccgac
     9901 gtggtggaat tggtagacac actagcttga ggggctagcg ccgcaaggtg tgggagttcg
     9961 aatctccccg tcggcaccaa atgtcgtcac gcgctggtac aatgcgggga tactccaggg
    10021 ggcaggccgc acgccggtct gtccccttcg tttgcgggcg agcctttcga cggatgcaca
    10081 agcgccgccg gcgaccttct gcaactggac gaacatgctg tcgaactatc tgccgatcct
    10141 gatcttcctc gtactcgcca cggcgctggc cgtggtcctg ctggtgctcg ggacggggct
    10201 cggccgctac ttcgcgcgct tccatcagga tccagccaag cgctcgccgt acgagtgcgg
    10261 cttcgatgcc ttcgaggaca gccgcatgaa gttcgacgtg cgctactacc tcgtcgcgat
    10321 cctcttcatc gtgttcgacc tggagatcgc cttcctgttt ccctgggcgg tggcgctcag
    10381 caagatcggc ggtttcggcc tcatcgcgat ggccatcttc ctcggcatcc tcgtcgtggg
    10441 attcatctac gagtggaaga aaggggcgct cgagtgggac tgagccggga gcagaccacg
    10501 tgagtgccgc tgttcagccg ccactcgaca tggacggctt cgcacagcga ggctttctca
    10561 ccacgcgcct ggacagtctc gtcaactggg cgcgcaccgg ttcgctctgg ccgatgacct
    10621 tcgggctcgc ctgctgtgct gtcgagatga tgcacgcggg cgcatcgcgt tatgacctcg
    10681 atcgcttcgg cgtcgtcttc cgtccgagcc cgaggcagtc ggacgtgatg atcgttgccg
    10741 gcacgctggt gaacaagatg gctcccgcac tgcgcaaggt ctatgaccag atggccgagc
    10801 cgcgctgggt catctcgatg ggatcgtgcg ccaatggagg cggctactac cactactcgt
    10861 acgccgtcgt gcgcggctgc gatcgtgtcg tgcccgtgga cgtctatgtc ccgggctgtc
    10921 cgcccactgc cgaggcattg gtctacggca tcctgcagtt gcagaagaag atcgagcgga
    10981 ccagcacggt agcccgacag tcccgctaga tggagccctc gaaaaacatc gaagcattgg
    11041 cgctgcaggt cgatgcgcac ttgaaggagg gcgtgcggcg cgtgcccgcg ctgcccgacg
    11101 agctcgccta tgaagccgag ccggccgcgc tgctgtcggt ttgccggaca ctgcgcgacg
    11161 ctcccgagct gaaattcgag atgctgatgg atctggcggg ggtggattac ctgcactacg
    11221 gccgcgacga ctggcaaacg acgaccgcga ctcattcggg cttcagtcgt gcgcgggtga
    11281 tgcgcacaca gccacccgac ccggaaatgg cgggtcgttt cggtgtgacc taccagcttc
    11341 tgtccatcac gcacaaccga cgcatgcggc tgcgcgtgaa gtgtcccgac acgcaggagc
    11401 cggtcatcga ttcggtcatc gatctctggg ccgcagccaa ctggttcgag cgtgaagcct
    11461 tcgatctgta cggcatcctc tttcgcggcc accctgacct gcggcgcatc ctcaccgact
    11521 acgggttcat cggccatccg ttccgcaagg atttcccgct catcggcaac gtggaagtcc
    11581 tgtacgacac ggaaaagcga cgcgtggtct atcagcccgt cagcatcgtc ccgcgcgtgc
    11641 tggtgcccaa ggtgatccgt cacgaccccc gctatgagcc ggcgctccgg gacgtggtgc
    11701 cggaggaatt gcgggggccg cgctgatgtc cgagattcgc aactacacgc tgaatttcgg
    11761 gccacaacat ccggcggctc acggtgtcct gcggctggtg ctggagatgg atggggagac
    11821 catccagaag gccgatcctc acatcgggct gctgcatcgc gcgaccgaga agctcgccga
    11881 gtcgaagccc ttcaaccagt cgatcggcta catggaccgc ctcgactacg tctcgatgat
    11941 gtgcaacgag catgcctacg tgctcgccat cgagcgtctg ctcggcatca ctccgcccga
    12001 gcgtgcccgc tacatccgcg tgatgttcga tgagatcacg cgcatcctga atcatctgat
    12061 gtggctcggg gcccatggcc tggacatcgg cgcgatgacc gtgttcctgc tgtgcttcaa
    12121 ggagcgcgag gagctgatgg atgtctacga ggcggtctcg ggcacgcgca tgcacgcgac
    12181 ctactacagg cccggtggcg tgtatcgcga tcttccggac acgatgccga agtatcaggt
    12241 ctcgaggtgg cgctcgcaaa aggaagtgga ccgcctcaac gaggcgcgcg ccggctcgat
    12301 gctcgatttc atcgcggcct tcaccgatcg cttcccggcg cgcatcgatg actacgagac
    12361 gctgcttacc gacaaccgca tctggaagca acgcacggtc aacatcggag tggtctcgcc
    12421 cgagcgcgcc ctgcagctcg ggttcagcgg gccgatgctg cgcgggtcgg gggtcgagtg
    12481 ggacctgcgc aagaagcagc cctacgaagt ctatgaccgc atgaatttcg atatcccggt
    12541 cggggtgcat ggcgactgct acgaccggta cctggtccgc atcgaggaga tgcgccagtc
    12601 gaatcgcatc gtgcgtcagt gcgtggattg gctcagcgcc aattccggcc cggtgatgat
    12661 cgatgaccgc aaggtgcgtc cgccgcggcg cgagcggatg aaggacgaca tggaatccct
    12721 catccatcat ttcaagttct ttaccgaagg ctacgaggtg cccgagggcg agacctacgc
    12781 cgcgatcgag gcccccaagg gcgaattcgg catttatctc atctcggatg gcgccaacaa
    12841 gccgtaccgg ctgaagtgcc gcgcccccgg ctttccgcac ctggcagcgc tcgaggaaat
    12901 gagcctcgga cacatgctcg ccgacgtggt cgccatcatc ggcacccagg atatcgtctt
    12961 cggggaaatc gaccgttgag cagcgagatc aattcggggg agaaggcgcc cgtgaccggc
    13021 aaagtccggc tgctctcgca gcacacgcgc gccgagatcg atctgtgggt gaggaagttt
    13081 ccgcccggcc ggcagcgttc ggcctgcctg tcggcgttgc gggccgcgca ggagcagaac
    13141 aagggctatc tcacaacgga tctgatggat gccgtggccg aatatctggg cctgccgccg
    13201 atccaggttt acgaagtcgc gaccttctat tcgatgttcg agctgcatcc ctgcgggcgc
    13261 caccatgtga gtatctgcac caacatctcc tgcatgctca acggcgccga ggaactggtc
    13321 gcgcacgccg agcgcaagct cgggatcaag ctcaaccaga gcactcctga cgggcgcatc
    13381 ttcctcaagc aggaagagga gtgcctggcc gcctgcacag gtgcgccgat gatgatggtc
    13441 gatcacatct tccacgagga cctgacaccc gagaaggtcg atgcgatcct ggatgagctg
    13501 aaatgagcgc agattacagc gaccgcatga acctggtggt gttcgagccg ttgaagctcg
    13561 agcgctcctg gaccctggag acctatcgca agatcggcgg ctatgaggtc tgggagcggc
    13621 tgctgagcga gaagccgccg cgcgagcaga tcatcgatgc agtcaaggcc tcgggactgc
    13681 gcggccgtgg cggcgccgcc tttccgaccg ggatgaagtg gagcttcatg ccgcgcaact
    13741 cgccggtgca gaagtacctc gtctgcaact cggacgagag cgagccgggc acctgccatg
    13801 accgcgacat cctgcgcttc aatccccact cgctgatcga gggactggcc atcgcaggct
    13861 atgcgaccaa caccacggtg gcctacaact acatccgcgg cgagtttctc ggcgagccca
    13921 ttccgcgctt cgaggcggcg ctggcggaag cctacgcagc gggactgctg ggcaaggacg
    13981 tgcgcggctc gggaatcgat ttcgacatcc acacgttcgt gggcgcaggt gcctacatct
    14041 gcggcgaaga aacggccatg ctcgattcgc tggaaggcaa gcccggcaag ccgcgcttca
    14101 agccgccttt tccggccaac ttcggcctgt acggtgcacc gaccacgatc aacaacaccc
    14161 agagcttcgc ctcggtgccg accattctgc gccgcgggcc ggagtggttc gtgggtctcg
    14221 gtccgaagaa ctccgccggt acggtgatct tctcggtctc aggccatgtg gccaagccgg
    14281 gcaacttcga gctgcccctc ggcattgcct ttgccgacct gctgcagatc gccggcggca
    14341 tgcgcggtgg ccgcgcgctc aaggcggtga taccgggagg cgcctcggtg ccggtcctgc
    14401 ccgccgagat cattctcaag accaacatgg actatgactc gctcaaggcc gccggctccg
    14461 ccatcggctc ggcggcggtc atggtgatgg atgagaccac ctgcatggtg cgggtcctgg
    14521 agcggatctc gcgcttcttc atgtcggaat cgtgcgggca gtgcaccccg tgccgcgaag
    14581 gcaccggctg gatgaaccgg ctcatccggc ggatcatggc aggcgatgcg cgccgcgagg
    14641 agctcggtct gctgctggat gtggccaaca ggatcgaggg tcacacgatc tgtgcgctcg
    14701 gggatgcttc ggcatggccc gtgcagagct tcctcaagca ctacaagcac gagttcgagt
    14761 acatgatcga ccatggcggc cgcagcatgg tcagcgagca gctcggagcg cgcgcggcat
    14821 gagcgcggag gaattcgtca acctggaggt ggacggggtc cccctgaagg cacgcaaggg
    14881 cgagatgctc atccgcgtca ccgatgcgca taacatctac gtgccgcgct tctgctatca
    14941 cgacaagctg agcatcgcgg ccaactgccg catgtgcctg gtcgaggtcg agaaggcgcc
    15001 gaagccgatg ccggcctgcg ccacgccggt ggtggagggg atgaaggtgt tcacgcgctc
    15061 accgcgcgca atcggtgcgc agcgcgcgac gatggaattc ctgctgatca accatccgct
    15121 cgactgtccg atctgcgatc agggcggtga gtgcgagctg caggatctgg cgatgggctt
    15181 cgggcgcgac ctgtcgcgct ttcacgagcg caagcgatcg gtccccgacg agaacctcgg
    15241 ccccctgatc gcaaccgaca tgacccgctg catccactgc acgcgctgta tccgtttcac
    15301 ccaggagatc gccggctatc aggagctcgg catggtcggt cggggcgaac agatgaaggt
    15361 gcgcacgtac gtcgagcaga ccgtgcatca cgagctttcg ggcaacgtca tcgacctgtg
    15421 tccggtcggt gcgctcgtct cgaagccgta ccgcttcagt gcgcgggcct gggagatgat
    15481 ctcccatccc ctggtggccc cgcacgaccc cgtcggatcc aacctcttcg ggcatgtctt
    15541 gcgcggcaag ctgatgagag tcattccgcg cgaaaacgag gcgatcaacg agacctggat
    15601 cagcgaccgt gaccgcttca gctacgaagg cgtctacagc ccggagcgca tcgagcagcc
    15661 gatgcttcgc cgcgggagtg gcccggatgc cgaatgggtc gagaccagct gggaagatgc
    15721 gctggcgaag gtcgtcgagg gtctgcggcc gcgggcggcc gagctgggag tgctcgccag
    15781 cagcaccagc accgtggaag agctctacct gttggggcgc ctcgcccgtg ggctcggcac
    15841 cccccacatc gatcaccgcc tgcgccagcg tgactgccgt gatcaggagg ccgatccggc
    15901 ctttccaggc ctgggcatgc gcatcgaggc ggtggactcg ttaagcgcac tgctggtggt
    15961 gggcgccgat ctgcgccggg aggttcccat ccttgcgcac cgcgtgcgca aggcagccct
    16021 ccgcggggcg aagatcgcct tcctcgggcc tgcaaagagc gaatacctgt ttccggtcgc
    16081 agccaccgtg gtttcctccc ccgcacagca ggtcacggag ctcgcggcag tcctcagtgt
    16141 agtggccgag gcgaccagca ggacggtccc tgcccatctc agcgatatcg tccgtggcgc
    16201 ctcggttgcc gatacccatc gcgcaatcgg caccgcattg ctctcgggcg agcgccgggc
    16261 gctctggctg ggagctctgg ccctgcgcca tccggcgtat gcggatctgc gcgcgctcgc
    16321 ggccgcgatc gccgagctcg ccggcgcctc cctgggcgtg ctggcagaag gcggcaacgc
    16381 cgccggtgcc tatctggccg gcgcggtccc gcatcgcgaa gccggtggcc ggccggcgag
    16441 ccggaccggc atggccgccc tcgagatgct ccaacaccca ctgaaggcct atctgctttt
    16501 cggcggagtg gagccctgga tcgactcggc gagcgcggac agcgcacagt cgctcgcacg
    16561 ttcggaattc gtggttgcca tgactccgtt cgcaaacaat cagctgcgcg ctgtggccca
    16621 tgtcctgctg ccgatcggga ccttcgccga aacctccggg acctacgtca acatggaagg
    16681 cctgtggcag agccagacgg gcgccgcgtt gccggtcggc gcgtcgcgtc cgggctggaa
    16741 ggtcctgcga gtgctcggca accttctcgc gctcgaaggt ttcgactatc agtcctcgga
    16801 agaagtccgg gaggagttgc gcgcgctgtg cgcggagccg gatgcgacgc agtaccgggg
    16861 cagtcatcga gtcgcccagc agcccgggag cgcggagatc ctggatctca acatgtatca
    16921 ggtggatgcc ctcgtgcgcc gcgccccgtc gctgcagaaa acgcgcgagg gccgcacctt
    16981 gcccgtgacc tactagggga aggacaggac ccgcgtgctg cagaccctct ccaacgcatg
    17041 ggcttcgctg ccggactacg ggcgaacgac cgtctggacc gtggtcatca tgatcgtcct
    17101 catcatttgc gtggccctgc tgacgttatg ggaacgcaag gtcatcggct ggatgcagct
    17161 tcggcgcggg cccaaccggg tgcgcatctt cgggctcgct cccggcctgg gtcagccttt
    17221 cgccgacgtc ctcaagctgc tgatgaagga agtcatcatc ccggcggatg ccaacaagat
    17281 cctgttccgg ctggccccgg tgattgccct ggtgccggcc ttcgccgcct gggccgtgat
    17341 tccgctgtct cccgaagtgg tcgtctccaa ggccaacgcg gggttgctgt atctgctgtc
    17401 gctgacctcg atgggcgttt atggcgtgat cattgcgggc tgggcggcca actcgaagta
    17461 tgcatttctg ggcgccatgc gctcggcggc gcagatggtc gcctacgaga tcgcgatggg
    17521 ctttgcgctc gtgggcgtga tcatcgccgc gcagagcctg aacctggggg atatcgtcga
    17581 gcgccagcag ggcggctttc tcgcctggaa ctggttctgg ctgttcccgc tgatgatggt
    17641 gtacttcatt tccggggtcg ccgagaccaa ccgcgcgccc ttcgatgtgg ccgaaggcga
    17701 gtccgagatc gtcgccggct tccatgtcga gtattcgggc attgctttcg cgctgttctt
    17761 cctcgccgaa tacgccaaca tgatcctgat ctcggcgctg acagcggtgt tcttcttcgg
    17821 cggctggctg tcacccttcg caggctatct cggcgactca tggctcgcgc gcccgagctt
    17881 tctctggctc ggcctcaaga tcttctgcgc gctgtttctg ttcctatggt ttcgcgcgac
    17941 gttcccgcgc tatcgctacg accagatcat gcgcctcggt tggaaggtgc tcatcccggt
    18001 gaccatcgtg tggatcggtg tggaaatgct cctcgcgtgg ctgcgtgtcg gaccctgggc
    18061 gcatcaggcc ctgtgggcgc aatgagcagg taggtcagca tggcattcaa cccgatcaag
    18121 acgctgctgc tgacagagct catccagggg ctctgggtga cgcgtcgcac tttcttcagc
    18181 cgcaagtaca ccctcaacta cccggaggag aagacgccgc aatcgcctcg cttccgcggg
    18241 ttgcatgccc tgcgccgcta tccgaatggc caggagcgat gcatcgcctg caagctgtgc
    18301 gaagcggtct gtccggcggt ctgcatcacg atcgactccg aggtggcacc ggacggaacg
    18361 cgccggacca gccgctacga tatcgatctg ttcaagtgca tctattgcgg cttctgcgag
    18421 gaagcctgcc ccgtggatgc catcgtgctg acccggatcc acgagtacca catggagcac
    18481 cgcggggaga acatcatgag caaggacaag ctgctggccg tgggtgagcg gtacgaggcc
    18541 atgatagcca ccgacaaggc ggccgacgcg ccataccgat aagagcgttt gcatgctggt
    18601 cgaagtcctg ttctacgtgt tcggcaccat cctggtcgcg ggggctctgg ccgtcatcat
    18661 ctcgcgcaac ccggtgtact cggctctctc gctcgtcctg tgcttcgtca cttcggcggc
    18721 catctggctg ctgatcgaag cggagttcct ggcggtgatc ctggtgctgg tctacgtcgg
    18781 ggcggtcatg gtgttgttcc tgttcgtcgt gatgatgctc gacatcgatc tggaggagct
    18841 gcgcacgggg ttcactcgct ttgcctggct cggatggctc acggcactgg ccgtcatcgt
    18901 cgagatcgtc ggagtggtct gggtgcgcgg cctcggcatt gatgtcaccc gaagcccgac
    18961 gccgacggct gcgaactaca gcaacacgct cgcgctcggg cgcgtgctgt acaccaagta
    19021 cgcctatccg ttcgagctgg cagcggtgct gctgttggtg gcgatcatcg cggcgatctc
    19081 cctgacgatg cgcaaacgcc ccggtctgaa ggctcaaaac atcgcaaagc aggtcgcagt
    19141 gaacagcaag gaccgcgtgc gcctcgtcaa gatggaagcc gagaagcctg ccgaaggcgc
    19201 ggccgacctg ggccagccgg cgcaagcgga gcagaaggcg caatgatcac cctcgcgcag
    19261 cttctgacgc tctcgtccat cctgtttgcg ctggcggtcg ccgggatttt cctcaatcgc
    19321 aagaacgtga tcctgctgct gatgtgcgtc gagctgctgc tgctggcggc caatttcaat
    19381 ttcatcgcct tctcgcgcta cctgggggac atcaacggac aggtgttcgt gttcttcgtg
    19441 ctgaccgtgg cggcggccga gtcggctatc gggctggcga ttctcgtggt gctgtttcgc
    19501 gagcgccgca gcatcaatgt cgaagatctg aacaagctgc ggggctgagg cgcgcatgaa
    19561 tccggacatt ctgatcgcca tcgcggtgct ccccttgctc ggagccctca ttgcggggct
    19621 cttcgggcgc gtgatcggcc gtgccggagc ccacaccgtt acgatcgccg gcgtcgccgt
    19681 ttcctgtgct ctgtctctgt acattctcaa gcagagctac tgggacggcg tcgcaacgtt
    19741 cagcgggccg gtctacacct ggctcgtcag tgacggcgtg cacctggaag tcgggttcct
    19801 gattgaccgg ctcaccgcgc tgatgatggc ggtcgtcacc ttcgtatcgc tgtgcgtgca
    19861 cgtctatacg atcggctaca tgcacgacga tgacggatat acccgcttct tcagctacat
    19921 ctcgctgttc accttctcga tgctcatgct cgtgatgtcg aacaacttca tgcagctgtt
    19981 cttcggctgg gaagccgttg gtgtcgtctc gtatctgctg atcggtttct ggtacacccg
    20041 gccgagcgcg atcttcgcaa gtctcaaggc attcctggtc aaccgcattg gcgacttcgg
    20101 cttcgttctc gggatcgcgg gtatcttcta cttcacgaac tcgctcgcgt atgccgatgc
    20161 attcgccgcc gcaccgcaga tcgcgcacgc gacccttccc ttgagctcga acaccagcgt
    20221 cgaagcactc acgctgatct gcatctgtct gttcgtcgga gcgatgggaa agtcggcgca
    20281 ggttcccctg catgtatggc tgccggactc catggaaggt ccgactccca tctcagcgct
    20341 gatccacgcc gccaccatgg tcaccgccgg gatcttcatg gtggcgcgca tgtcgcctct
    20401 gttcgagcgt tcggacacgg cgctgtcctt cgtcatggta atcggcgcga ccaccgcttt
    20461 cttcatgggg ctgctcggca tcgtcaacaa tgacatcaag cgtgtcattg cctattcgac
    20521 tctctcgcag ctcggctaca tgactgccgc cctcggggct tcggcctaca gcgcggcgat
    20581 cttccatctg atgactcacg cgttcttcaa ggcgctgctg ttcctggcgg ccggctcggt
    20641 gatcatcgcg ctgcatcacg agcaggacat gcgcaagatg ggcgggctgg cgaagtacat
    20701 gccgatcacg gcggtcacga gctggatcgg ggcgctggca ctcatcggca cacccttctt
    20761 ttccggcttt tactccaagg actcgatcat cgaggccatc ggcgaagcgc accgacgggg
    20821 tgcgacgtac gcgtactact gtgcactggg tggtgtattc atcaccgcgc tgtacacgtt
    20881 ccgcatgatc ttcctgacgt ttcacgggcc cgagcgtttc cgcagtcccg aggccgtgca
    20941 cagttgcgca tctgatcaga gcatcgggca tgaggagact gagatgtccc cggcggccca
    21001 tggggaggct gcgtccactc acgccgcatc agatcatgga tcacacgagc cgcctcacga
    21061 aagtccgctc gtggtaacgc tcccgctgat cgccctcgcc attccgtcgc tgctgatcgg
    21121 gatcttcacg atcggaccgg tcctcttcgg cgattatttc ggcagctcca ttcaggtact
    21181 gcagcagaac gatgtggtgc gggagcttgc gcgcgagttc catggacccg tggcctttgc
    21241 cttgagcggc ttcaccaagc cgcccttctg gcttgccgcc gcaggcgtgt tcaccgcctg
    21301 gctctgcttc ctccatcgcc cgcaactcgc cgaccaggcc gcccgagcgc tgtcgccgct
    21361 gcggacgctg ctgatcaaca aatactactt cgactggttc aacgaacgcg tgctcgccac
    21421 tctggctcgc ggagtcggca tcagcctgtg gaaggccggc gaccaggtac tcatcgatgg
    21481 ggccgtggtg aacggctccg ccgcgacggt gcgctggttc ggcagcgtcg tgcgcagggt
    21541 tcagaacgga tatctgtatg cctacgcgtt ctggatggtc atcggcctgg cggtgctgct
    21601 gggctggttt ctcgagcact cctaactcat tggcgggcac tgaatgcacg ggtctctgtc
    21661 ggtcctgatc tggctgccca tcgccgcggg gatcgcggtc ctgctgctgg gggagcgcaa
    21721 catcgccgcc ggacgctggg tcgcactcat tgcctcgatc gtgacgctgc tggtgtgcct
    21781 gccgctgctg agccatttcg acaccagcac cgcggccccg caattcaccg agcacgtgct
    21841 ctggatcagc cgcttccatg cctattacgc gctcgggctg gatggcatct cgctcccgct
    21901 cgtcgtgctg accgcattcg tgacgattcc ggtgatcatc gccgcctgga cggtcatcga
    21961 ggcccgtccc gcgcagtact tcgcctcgtt cctgatcatg gaagggctga tgatcggggt
    22021 gttctccgcc accgatgcgc tgctgttcta tttcttctgg gaggcgatgc tgattccgat
    22081 gtttctcatc atcggcatct ggggcggtcc acgccgtgtg tacgcgacca tcaagttctt
    22141 cctgtacacg tttctcggct cggtcttcat gctcgtcgcg ctcatctaca tgtacgtgaa
    22201 ggccggcgat tactcgatcg cgagcttcca gggactgccc ctcacgctca tcgagcagcg
    22261 ctggatcttc gtcgcgtttc tgctggcctt cgccgtgaag gttcccatgt ggccggtgca
    22321 tacctggctg ccggatgcgc acgtcgaggc gcccaccggc ggctcggtga tcctggcggc
    22381 catcatgctg aagatgggcg gctatggctt cctgcgcttc agcctcccga ttgcgccgga
    22441 tgccagccgg gagctggatc tgctgctgat ctcgctgtca ctgatcgcgg tggtgtacat
//
LOCUS       DASRHN010000399        22206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_483037, whole genome shotgun sequence.
ACCESSION   DASRHN010000399 DASRHN010000000
VERSION     DASRHN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22206
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483037"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VFB37_16150"
     CDS             complement(<1..1944)
                     /locus_tag="VFB37_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24029.1"
                     /translation="MRPLLCAAVGLALTLLASAPAGARRHDPEKLPDTRIRDLHYGDV
                     LFYLFQDDDFEAITRLTAYQHWNRIPHHQDEAQLLLGGLYLSLGMHNEAGERFQSLLN
                     QDVPTGVRNRAWFYLAQVWYARGYLDKADEALRRIQGKLSTELEAQKEHLFANVLMHE
                     GKFDEAIQLLSTSKTSPLWSAYARFNLGVALVREGRLADADPFLTAVGTSYVESNELL
                     ALKDRANLALGFAYIQANQPAKARVALERVRLKGPYSNKALLGMGWADAALGDYRGAL
                     SPWLELRGRNLLDAAVQESYLAVPYAFSKLNASAQSAEYYESALKSYETETGQLDEAI
                     ARVRSGQMLEDVITSERDSHYGWFWQLKEVPDAPESRYLYTVLAGHDFQEGLKNYRDL
                     RYMGHTLDRWSDSMEAFADMIDTREHAYAERLPRVDALLSSGTMDRLARRRSELESRV
                     NAIEASKDVAAVGSTEEREQWARIRRLEAALAGAPDTPENADLRERLRLVKGVLYFRL
                     DESFKARMWQQHRSIKDLDLALQEAQSRWIRVDRARKSVPTNTGEFAARVAALKTRID
                     ALQVRLAATEQKQGAYLAQVAVGELEEQKSRLATYQVQARFALATMYDRAANADQAQE
                     GQTPQPTQQGAPAPPPSQPEAKPP"
     gene            complement(1967..2713)
                     /locus_tag="VFB37_16155"
     CDS             complement(1967..2713)
                     /locus_tag="VFB37_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24030.1"
                     /translation="MPYPSLHRPIPARWAAALAALALLLSGPWVLPSAAFAQSTAPTG
                     PAASHATPPPAQAPASGAGSAAVAPAANAPPAAPAAAAPAAEAPPADAPQGESQADTR
                     GLDQEVQGLKKDVVDLNRDLFVLEEELLFPANTQVAVFLSMDVGDFFALDSVTLKIDQ
                     KEVINYLYTPREVDALLKGGVHRLYLGNLKVGKHELVAFFSGKGPNERDYKRGATIKF
                     EKGIGAKYLELKINDRQRRQQPEFEIKDWE"
     gene            complement(2725..3234)
                     /locus_tag="VFB37_16160"
     CDS             complement(2725..3234)
                     /locus_tag="VFB37_16160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZO24031.1"
                     /translation="MPRRFCVGWRWLLAIALGAAAQADPAYPLIGHEAPDFALRAIAG
                     ENVRLSEHRGDVVVLSFWGSRCTPCRAQLAALNRSFGTYHSAGMQIYGISVDDDQARA
                     IAYARGEHLTFALLLDPGKTVSRSYEVDNLPMTLLVDRSGVVRHVFRDFDARSEGLYL
                     QQLRELLNE"
     gene            complement(3389..5020)
                     /locus_tag="VFB37_16165"
     CDS             complement(3389..5020)
                     /locus_tag="VFB37_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24032.1"
                     /translation="MLIRKKPPRPHAPGEPFLHADHPRPVTRRQMLAAGLTSAPAIVM
                     APAWLAALLKPGRAKAALALDMQALLPSGQCNLSAPASGIPVVCFDLAGGANLIGSEV
                     IVGLKGGQANFLSTAAYGKMGVPGNMVPTANSAFVSSALGLLWHSDGAILRGILTKAT
                     TPATALGTNGAVIAAMSQNDTNTNPHNPMYGIAEAGGKGTLLNLIGSQSTVSGGNSAA
                     PMAMIEPQLQPTKIASPGDATALVSTGGASADAVDVAILQSQARISGGSTPYDPSNTG
                     GTEFTGALSPPQGATAGTLLYPTDPASDVQLKNQLRCAYVQSANTADVFGSPAALDPT
                     QDPLITGGAAPIFTAADFQDSDIAATASVMKLVIDGFAAAGTITLGGYDYHDNTRASG
                     ETKNFKAGQMIGAVLEYAQRKKQPVMIYVFSDGSLTSSSMVDTSTAGRDKLAWQGDNQ
                     SVASTFFLVYSPKGRPQPRNGAASQQIGWYTSDGAVDPTSNPAANSVNQLVETVILNY
                     LGLQGKDGTFSTLFPSQGLGSASAQAALTAFEPIV"
     gene            complement(5042..7579)
                     /locus_tag="VFB37_16170"
     CDS             complement(5042..7579)
                     /locus_tag="VFB37_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HZO24033.1"
                     /translation="MKRKHLMYRTEPTFAGVGTLRVAAALALAAVLAACTGGGAPTAV
                     NQVTSNTTTANAYTGPAPQTADIQAFQINLWQNIRVANRCGGCHHEGGQSPMFARSDD
                     VNLAYQAALPLVDASNPSQSTLVLRVGSGHNCWVADPNACAQTMLTWIDNWLGGGSAV
                     ATKITLTPPPVQSVGGSKLFPADSTAFQQLIWQPILTQFCSNCHRPDSSTAQAPYFAS
                     SDPNQAYLAAQAKINLDVPSESRFVERLQSELHHCWATTPGGPPDCPGSAAKMLAAIT
                     AYANTIPTTQVDPSLVISKALTMEQGTIAAGGSRYEANIVAKYMFETGTGNTAYDTSG
                     VSPSADLTLYGPVTWAGGWGINVPMGGKAQASTSSSQKIAQLIQATGEYSIEVWAAPA
                     NVTQTDAYILSYSGSDKTRDMTLAQHMQQYEGMTRSTVTDSNGSPPLLTAAANMNAQA
                     ALQHIVLTYDPINGQKLYVNGVFTGDVDPNKGAALSNWDNTFALVLGSEVTGKEQWTG
                     LIKFAAIHSRALTPTQIQQNFAAGVGQSYYLLFDVSSLSGIAQSYILMQASQYDSYSY
                     LFNKPTFFSLNSKAAPSSLVIKGLRIGMNGSVPTVGQSYSTLSTTVGPPGYTAANGQL
                     LSNVGAVLAVDAGMASDMFFLSFDQFGSHQHVFVEPTYTLQPPVPSTTPQPDVGVATF
                     ERIYHAMSNITGVPFTNATVAALYNTEQQSMPAGPLVTAFVSSEQTAISQLAQAYCGQ
                     LVGTQALRDNFFGTGLDASIGATAGSFFGSSSSPNTANINLVVTPLVNATVGTAAHQQ
                     TAAGMTTELNHLLTLIPTLPGEASTTVSKATQAACTAALGSAAVVLQ"
     gene            complement(7592..8809)
                     /locus_tag="VFB37_16175"
     CDS             complement(7592..8809)
                     /locus_tag="VFB37_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24034.1"
                     /translation="MNSTRFHSGKHPAARLSRAASGACMLAVLTQLAAPAFAGPNEQA
                     ERIYERIAGVPPTPAVLASMAQAITNNPGQTGLMQAAAIATSAPTFYNITLKNMVIPW
                     TNRDGTVFAPLNDYAATVIGMVHDDVPFNTALSADILYTVNASGLPAPSVSNNDHYAM
                     AETNGVDLSSTLRATTQSATYGIPTAATAGLLTTRGATSAFFINGTNRAMFRFTMINH
                     LCNDMPTLMDTSRPTDRIRQDVARSPGGDSRTFLNNCVGCHSGMDPMAQAFAYYNFDA
                     TVGTQGALVYTPGKVQPKYLINAQNFSQGFVTPDDSWSNRWRAGPNSVLGWDSSLPGS
                     GSGAKSLGQEIESTDAFAQCQVTKVFQTVCFRAPTNAADIATVGSIKASFKAGGYHLK
                     QVFQQAAAACPGT"
     gene            9178..11892
                     /locus_tag="VFB37_16180"
     CDS             9178..11892
                     /locus_tag="VFB37_16180"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24035.1"
                     /translation="MNVNAGIVTEIMSRVVQPGRAASAQRLLPAVLAAVLLALLSGCS
                     GNAHIDVANSQVADPATVDFPIFYVKRTIPAKPDDLRVMRDLVPTADLFKRDSASPSA
                     NETNITSRVTANAAYDVKDVDVSADGTRVAFAMRGPITMNMKAKDPPSWRIWEYVIAT
                     DLLHQVIDPTTDPDPPTVNDESPHYLPDGRIVFSSTRQTQSQGVLLDEGKPQFEAQDE
                     DRTESTFNLHVMNPDGSDVHQITFNQSHDRDATVLSSGRVLWTRWDHAPGHDAMHLYS
                     ANPDGTDLELYYGANSHMTGTNNTVIEFVRAREMQNGDILTLTRPYTDASATQKIDFG
                     GNLTIINGKDFVENTQPLLANAGLKGPAETPATPNNVSTIPGPSPGGRFDSAFPLWDG
                     TSRILVSWSQCRLLNTANTIVPCTASALADPNVMMAPPLYSVWMFDPNQNTMLPVMPP
                     VEGVMVTDVIAAQPRALPNVILDKIPGVDLDQDLVSAGVGVLDIRSVYDFDGVDTAKP
                     NIPTVADPAKTPASQRLARFIRLEKAVSIPDRTLVKLSGAAFGASNYMREIMGYAPVE
                     PDGSVHIEVPANVAFMMSVLDANGRRIGPEQGVWLQGLPGEVISCNGCHVPATPQHPI
                     SHGRAGLFNSIYAGAMTTGTAFPHTIATGPGAFLPNAGETMAQARMRTSCTNDSPRCA
                     QMVPSVNVLYTDVWTNPAQATPGKPITYRYDDPTFMTPIPTLAACVGTWAANCRIVIN
                     YPQHIQALWDLTRQTVVAGVVTADHTCSQAGCHNPKNAAGVAEVPAGQLDLTNAASNA
                     EPQQFVSYQELLFPHAEQEVNMGALQDVPGPPGANGAPTTIPVGPFMNAGSANGALSN
                     AFLGRFAPGSGSTHAGYLSPAELRLISEWLDIGAQYFNNPFDPAVPVN"
     gene            11948..12691
                     /locus_tag="VFB37_16185"
     CDS             11948..12691
                     /locus_tag="VFB37_16185"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZO24036.1"
                     /translation="MRTLLFVGLLLSLCLPAPAFSREREHLQVFITAPYLELRTGPGR
                     GYPVFHIAERDESVEVLFRRTDWFKVRTERGVEGWAAQRDMLRTVLADGTPFRFELGD
                     RSGFTSHRFEMGIFAGDYGGATLISGYSSFSFNSQLAAEASFGQFLGKFTNGVVADFG
                     FSHVLVPEWRLSPFFMLGTGLVHVSPKATLVVPSERTDQTAYVGGGLRFYLTRRFFLR
                     AEYKSHIVFTKRNANEEVDEWKLGFAFFF"
     gene            12661..13395
                     /locus_tag="VFB37_16190"
     CDS             12661..13395
                     /locus_tag="VFB37_16190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HZO24037.1"
                     /translation="METGIRILLLTVVLVGLPGCASVRNWFHHRSEVRQARAEAREEA
                     AQADAQAAQQDSEASPPRVIEPEVQRRKIKVPRIHSSNVELGLQYGFISIEDFGTQST
                     YGLSAAYHVTEDFFFLGEAGRAYAGRTSFETLGGNIQLLTSAERRFTYYDLSLGYNFL
                     PGEAFLGRGVALSSAFYLVGGIGSTQFAGDDRFTVNFGAGYRVLPADWLAVHIQVQDR
                     VFRTDLLGVSKLTNNMEATIGATVFF"
     gene            13413..13925
                     /locus_tag="VFB37_16195"
     CDS             13413..13925
                     /locus_tag="VFB37_16195"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZO24038.1"
                     /translation="MKKSTHAAKGATLAMFALALLAVVPAMGADPSGPAPAFTLAQRG
                     GGNLSLAKYKGQVVMLNFWASWCGPCRQEMPLLEDIYKKYGKLGFTLIGVNVEPDSNA
                     ANDWLKQTPVSFPIAYDTQSQVSKLYDVQGMPSTVIIDRKGNLRVLHRGYKPGDENGY
                     LDSIRALIRE"
     gene            13953..14258
                     /locus_tag="VFB37_16200"
     CDS             13953..14258
                     /locus_tag="VFB37_16200"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HZO24039.1"
                     /translation="MSSTFNTLAQTARLVAQAIPRRPGLPRLAALGLIAMALEVALSG
                     CSPIEPWVKPYEREHFADPIMAFNRDPVSSVYREHVFESREGARGATGGVGGGCGCN"
     gene            14249..15490
                     /locus_tag="VFB37_16205"
     CDS             14249..15490
                     /locus_tag="VFB37_16205"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HZO24040.1"
                     /translation="MQLSGAPGRLEAALRRHSGTLLALCLCVAPALAGVLPEDRTDLL
                     YHRYEGGGITVQGPSLLIQKKLTDNLALTANYYEDMISSASIDVKLAASPYKETRKQK
                     SAGFEYLHGKSTYSAGLINSVEPDYRSNTSYYSISQDMFGDLTTFTLTYKRGWDKVFK
                     DGKVAGRIENVPFPDEAGVPDPGALSQDADHRGYTVGLSQILTRNLIATLNYEVLTDQ
                     GYLQSPYREILFLDPTQALGYSKADQIYPNTRTSNAAAFEAKYYLPYRAALTGQYRYF
                     QDTWGIRAHTFEVGYTQPVWRRLILDGSVRFYTQNAASFYSDLFPRAAFQNFEARDRE
                     LAAFNSYSLGLGASWDFPKPPVPWINHSSLSMRIDHLLIDYKDYRNALLIDPAAGIRP
                     GDEPLYKLNANIFQLFMSVWF"
     gene            15586..16434
                     /locus_tag="VFB37_16210"
     CDS             15586..16434
                     /locus_tag="VFB37_16210"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZO24041.1"
                     /translation="MRSRTRLFTVLTALVLCAGAQGAWAQAREEGRLLMASQVLDELR
                     NGRDQYVPEKLLERAYAIAIIPDMTKIAFFAGGRRGHGVLVVRDKNGRFTSPVFITMT
                     GGSFGWQWGVQSTDIMLVFTTRRGVEGITGGKMTLGADASVAAGPVGRSASASTDTSF
                     KAEVYSYSRSRGVFAGLALDGTALTIDDDANAAFYRKPGVMASDIIAGNIRSDDESAR
                     RFLGAVNNSTQSESASSATPATASAPTPELPQGAPRSDAPAPRSQSSGAQSFPLEDPK
                     PGAEPK"
     gene            16612..17970
                     /locus_tag="VFB37_16215"
     CDS             16612..17970
                     /locus_tag="VFB37_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZO24042.1"
                     /translation="MRDLTQGPISRQLLAMAVPIAAGMIFQTLYFLVDLYFVAGQGDT
                     ALAGVSSAGNVSFVILALTQMLGVGTVALVSHAAGRKDQAEANLVFNQSVCLSALLAC
                     VTLIAGYSLAPAYVRSVAADPHSAAAGITYLRWYLPGLALQFALVAMGSALRGTGIVQ
                     PTMIVQVLTVVLNTVFAPILIAGWGTHHPLGVAGAGLASTLAIALGVLFLWLYFARLE
                     KYVALHPPQWLPHPGTWRRILNIGLPAGGEFLLMFVFIGIVYWTIRHFGPTAQAGFGI
                     GSRVMQAIFLPAMALAFAASPVAGQNFGAARMDRVRATFRSAAFMSSTVMLALTLFCQ
                     WRPDLLIQAFSSDPAVVGVGAPYLRIISWNFVATGLIFTCSSLFQALGNTWPSLVSSA
                     TRLVTFALPAVWLGSQGHFELRHLWYLSVCTVSVQAVISLCLLQRQFRQRLVQSTPAV
                     AG"
     gene            complement(17979..19070)
                     /locus_tag="VFB37_16220"
     CDS             complement(17979..19070)
                     /locus_tag="VFB37_16220"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZO24043.1"
                     /translation="MSRPEQMTAVESTRAYERWLAKRLKILKPDLRLKHQRMQQDPFF
                     FMRASFYRWAQLWPLQCASLCLAPRVLGIGDLHVENFGTWRDGEGRLIWGINDFDEAT
                     TLPYANDLVRLCTSALLAIGESRLATPARLACESVLEGYVRSLKEEGAPVVLAERHRW
                     LRDLAVSRLKEQRTYWDRLLSLRPVTAVVPARVRAILRNALPERDIAFRIVHRQAGLG
                     SLGRERYTALADWRGGTIARETKPLTSSAWSWMSASREPRLQYSDVIRRAVRVPDPFL
                     SVRDGWVIRRLAPDCSRIELQSVAGIRDECRLLWMMGWETANVHLGTPRQRKGILRDL
                     ARRRPDWLLKAARRMRDATLEDWRHWSGR"
     gene            complement(19096..20961)
                     /locus_tag="VFB37_16225"
     CDS             complement(19096..20961)
                     /locus_tag="VFB37_16225"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZO24044.1"
                     /translation="MRTALKWLGVLIGAVALLCFALILQSWYFRPLTIGIFFERVLIQ
                     FLLEDPEAASSLGIFEQLGYRGFDGSLTDVSPAHEEKLAKMVRDDLATLHQYDRSSLS
                     ASQQLSYDVLEWFLQDQVNGQRWLYYDYPVNQLFGVQSATPDFMVQIHPLADQRDARN
                     YVSRLSDFGRQFRGLLQGLQLRESKGIVPPGFVIARVLAEMRAFIAVPPSANVLYVNL
                     HDKLAKLGNLSASERARLQDQARAAIEQTVYPAYRSLIAYFEHLYPKVAPDYGVWKLP
                     DGDAYYDYLIRSHTTTALNAEAVHEIGLKEVARIETQLDAILRSQGLEHGTIGARIAV
                     LDSDSRFQYPNDDHGRAQCLADYQRYINEIIRDLPREFAAPPALDIHVERVPPFKEKT
                     APGAYADPGSLDGSRVGVFYVNLRDMREIWKYQMRTLAYHEAVPGHELQGAIAQRLTD
                     VPVFRKVLPFTAYDEGWAVYAERLAWEMGFERDPLDNLGRLQAEMFRAVRLVVDTGIH
                     RMHWTREQAIAYTMEKTGMAEGEVVSEVERYYVVPAQALAYKIGMLKFLQLRAQAQER
                     LGPRFDIRQFHRVVLGNGPLPLGILEKQVNAWIARSRDDARGPSPQAAAQERSTP"
     gene            complement(20981..>22206)
                     /locus_tag="VFB37_16230"
     CDS             complement(20981..>22206)
                     /locus_tag="VFB37_16230"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HZO24045.1"
                     /translation="AESLAAQGTMVAGIDTAELFTSLEKDGGSCVAPDGDLENLSHYI
                     QAYYKLPTYFTPILVGHSAGATLAYTTLAQAPPGIFAGAVTLSFCVDLDLRKPLCQTQ
                     GLRFKPLAHGAGAQLLPPSQLHAPWIALHGTRDRVCPISRARAFVARTPGAKLIELPG
                     VTHNYSGTKGWLSQFDAAYASIASTQVQRLPVPPASLADLPIVEVEATGTPAPGNADS
                     FAVLISGDGGWAGIDKQVAGLLAEHGVPTAGVDSLRYFWTVRTPAGLAQDIDRIVRYY
                     AFHWKKKHAILIGYSQGADVLPFAVNRLPAPTRALIELTALIGLSDSAAFEFHVANWV
                     GAEDGGLPVPPEMDKLSSADTVCIYGDDDTDTICPKVSAQHASVVELPGGHHFGGNYE
                     PLAQLILDRAAARTQ"
BASE COUNT         3777 a         7264 c         7159 g         4006 t
ORIGIN      
        1 cggcggcttg gcctccggct ggcttggagg tggggcaggc gcgccttgct gggtgggctg
       61 cggtgtctgg ccttcctgcg cctggtccgc attggcagcg cgatcgtaca tggtcgcgag
      121 tgcaaaccgc gcctggacct ggtaggtcgc gagccggctc ttctgctcct ccagctcacc
      181 gacggcaact tgggcgagat aggcgccctg cttctgctcg gtagctgcga ggcgcacctg
      241 cagcgcgtcg atgcgcgtct tcagcgccgc gacccgcgcg gcaaactctc ccgtgttggt
      301 cggcacgctc ttgcgtgccc ggtcaacccg tatccagcgg ctctgcgctt cctgcagcgc
      361 gaggtcgaga tccttgatgc tgcgatgctg ctgccacatc cgggccttga aggactcgtc
      421 gaggcggaag tagagcacgc ctttgacgag tcgcagccgc tctctgaggt cggcgttctc
      481 cggagtatcc ggtgcgcccg ccagcgccgc ctcgagccgg cggattcgcg cccactgctc
      541 gcgctcctcc gtcgagccca ctgctgcgac atccttgctc gcctcgatgg cattgacgcg
      601 gctttccagc tcactgcgtc gccgcgccag acggtccatg gtcccggagc tcagtagcgc
      661 atcgactcgg ggcagacgtt cggcataggc gtgctcgcgc gtatcgatca tgtctgcgaa
      721 cgcctccatg ctgtcgctcc accggtccag cgtgtggccc atgtagcgca ggtcgcggta
      781 attcttcagg ccctcctgga aatcgtgtcc ggccagcacc gtatagagat agcgtgactc
      841 gggcgcatcc ggcacctcct tgagctgcca gaaccagccg tagtgcgagt cacgctcgct
      901 ggtgatcaca tcctcgagca tctggccact gcgcacgcgc gcgatcgcct catcgagctg
      961 acccgtctcg gtctcgtagg atttcagggc gctctcgtag tactcggccg actgagcgct
     1021 ggcattcagt ttgctgaagg cgtagggcac cgccaggtag gattcctgca cggctgcatc
     1081 gagcagattg cgaccgcgca gctcgagcca cggactcaga gcgccgcgat aatccccgag
     1141 tgccgcatct gcccacccca tcccgagcag cgccttgttg gaatagggtc ccttcagacg
     1201 cacccgctcg agggcgacac gggccttggc gggttgattg gcctggatgt aggcgaaccc
     1261 cagtgccaga ttggcccggt ccttgagggc gagcagctca ttcgattcca catacgaggt
     1321 gccgactgcc gtcaggaagg gatcggcatc cgccagccgg ccttcacgca ccagggcaac
     1381 cccgagattg aagcgggcat acgccgacca cagaggcgag gttttggaag tcgaaagaag
     1441 ctggatggct tcgtcgaact tgccctcgtg catcagcacg ttggcgaaca gatgctcctt
     1501 ctgcgcctcc agctccgtcg acagctttcc ctgtatccgg cgcagtgcct catcggcctt
     1561 gtccagatag ccgcgcgcat accacacctg ggcgaggtag aaccaggccc ggttgcgcac
     1621 accggtggga acatcctgat tcagcagcga ctggaagcgc tcaccggcct cattgtgcat
     1681 gccgagcgac agatagaggc ctccgagcag caactgcgcc tcgtcctggt gatggggaat
     1741 acggttccag tgctggtagg ccgtgagccg cgtgatcgcc tcgaagtcat cgtcctggaa
     1801 aagatagaag agcacatcac cgtagtgcag atcgcggatg cgggtatcgg gaagcttctc
     1861 gggatcgtgg cgccgggctc ccgcaggggc gctcgcgagc agggtgagcg cgagccccac
     1921 ggctgcgcag agcagcggac gcattgcctt gaaagccgac aaggcgctat tcccagtcct
     1981 tgatctcgaa ctccggctgc tggcggcgct gccggtcgtt gatcttcagc tcgagatatt
     2041 tcgctccaat gcctttctcg aatttgatgg tggcgccacg cttgtagtcg cgctcgttcg
     2101 gccccttgcc gctgaagaag gcgaccagct cgtgcttgcc gaccttcaga ttgcccagat
     2161 agagccggtg cacgccgccc ttgagcaacg catcgacctc acgcggagtg taaagatagt
     2221 tgatgacctc cttctgatcg atcttcagcg tgaccgagtc gagcgcgaag aaatcgccta
     2281 cgtccatgga aaggaaaacg gccacctgcg tgttggcggg gaacagcagc tcctcttcca
     2341 gcacgaagag atcgcgattc aggtcgacca cgtccttctt aagcccctgc acttcctgat
     2401 ccaggccgcg ggtatcggcc tgagattcgc cctgtggcgc atccgccggc ggtgcttcgg
     2461 cggcgggcgc tgctgcggcg ggcgcagccg gcggcgcgtt cgctgccgga gcaaccgctg
     2521 cggagcccgc gcccgaggcc ggcgcttgcg caggcggcgg ggtcgcatgt gaggcggccg
     2581 gccccgtcgg cgcggtgctc tgcgcgaacg ccgcactcgg cagcacccac ggtccactca
     2641 ggagcaatgc cagagcagcc agtgcggctg cccaccgcgc cggtatcggg cgatgcaagg
     2701 aggggtatgg catgggtctt ccgttcattc gttcagtaac tcccgcagct gctgcaggta
     2761 aagtccttcg ctccgggcgt cgaagtcccg gaacacgtgg cgcaccacgc cgctgcggtc
     2821 gaccaacaag gtcatcggca ggttgtccac ctcgtagctg cgactcacgg tcttacccgg
     2881 atccagcagg agcgcgaacg tgaggtgctc gccccgggca taggcaatcg cccgcgcctg
     2941 atcgtcatcg acgcttatcc cgtagatctg catgccggcc gagtgatagg tcccgaagct
     3001 ccggttgagg gccgcgagct gagcccggca cggcgtgcag cggctgcccc agaagctgag
     3061 gaccaccaca tcgccccgat gttcggacag ccgcacattc tcaccggcaa tcgcgcgcaa
     3121 ggcgaagtcc ggcgcctcat gtccgataag cgggtaggcg ggatcggcct gcgcggctgc
     3181 accgagggcg atggcgagca gccagcgcca gcccacgcag aaacgacgcg gcacggggcc
     3241 ttgcggccgt tgcaagctca aatatcttcc atccacacgg gaagcgaccg agggcgcggc
     3301 aaccctcgca cagcgaactt caaactgtca acgaaccagc gcccgcttcg agccggagat
     3361 ccggaccgac ggcccacgcc ggcccatctc agacgatagg ctcgaaggcg gtcagagcgg
     3421 cctgcgcgct tgccgagccg aggccctgcg aggggaagag cgtgctgaac gtgccgtcct
     3481 ttccctgcaa gccgaggtag ttgaggatca ccgtctcgac gagctggttg accgagtttg
     3541 ccgcgggatt cgaggtcgga tccaccgcgc cgtccgaggt gtaccagccg atctgctgac
     3601 tggccgcgcc attgcgcggc tgaggccggc ccttggggct gtagaccagg aagaacgtcg
     3661 aggcgaccga ctggttgtcg ccctgccagg cgagcttgtc acgaccggcc gtgctcgtat
     3721 ccaccatgct actcgaggtc agcgagccgt cgctgaacac atagatcatc acgggctgct
     3781 tcttgcgctg cgcgtactcc aggaccgcac cgatcatctg tccagccttg aagttcttgg
     3841 tttcgcctga ggcgcgcgtg ttgtcgtggt aatcgtagcc tccgagggtg atcgtgcccg
     3901 cggcggcgaa cccatcgatc acgagcttca tgacactggc tgtggcggca atgtccgagt
     3961 cctggaagtc ggcggccgtg aagatcggcg cggcgccccc ggtgatcaga ggatcctggg
     4021 tcggatcgag cgccgcggga ctgccgaaga catcggccgt attggcgctc tgcacgtagg
     4081 cacagcgcaa ctggttcttg agctgcacat ccgaggccgg gtcggtcggg tacagcagtg
     4141 tgcccgccgt tgcgccctgt ggcgggctca gcgcgccagt gaactcggtg ccgcccgtgt
     4201 tggacggatc gtaaggggtg ctgccgccgc tgatgcgcgc ctgggactgc agaatggcaa
     4261 cgtccaccgc atcagcggag gcgccgccgg tgctcaccag ggccgtggca tctcccgggc
     4321 ttgcgatctt ggtgggctgc agctggggct cgatcatcgc catcggcgcg gcggagttgc
     4381 cgccggaaac ggtcgattgc gagccgatca gattgagcag cgtgcctttg ccgcccgcct
     4441 cggcgatacc gtacatcggg ttgtgtgggt tggtgttggt gtcgttctgc gacatcgccg
     4501 cgatcaccgc cccattggtg ccgagcgccg tcgcgggcgt cgtcgccttg gtcaggatgc
     4561 ccctcaggat ggctccatcc gaatgccaca gcagaccgag cgcgctgctg acgaacgcgc
     4621 tgttcgcggt cggaaccatg ttgcccggaa cgcccatctt gccgtaggca gccgtggaca
     4681 ggaagttggc ctggccaccc ttgaggccga cgatgacctc cgatccgatc aggttggcgc
     4741 cgccggccag gtcgaagcat acgaccggga tcccgcttgc gggtgccgac aggttgcact
     4801 gaccgctcgg cagcagcgcc tgcatgtcca aagccagcgc cgccttggcg cggcctggct
     4861 tcagcagcgc ggcgagccac gccggagcca tgacgattgc gggagcgctc gtcagcccgg
     4921 cggccagcat ttgccggcgc gtaacggggc gcgggtgatc cgcgtggagg aacggctcgc
     4981 ccggagcgtg tggccggggc ggttttttgc gaatgagcat ttatgttctc tcctgaaacc
     5041 gttactgcag gacgaccgca gcgctgccga gcgcggcggt gcacgctgcc tgggtggcct
     5101 tggacaccgt tgtcgatgcc tctccgggca acgtcgggat gagcgtgagg aggtgattga
     5161 gctcggtagt catacccgcc gccgtctgtt gatgtgccgc cgtgccgact gtcgcattca
     5221 cgagcggagt gaccacgagg ttgatgttcg ccgtgttcgg tgagctgctg ctgccgaaga
     5281 aggagcccgc agtcgccccg atcgaggcat ccagcccggt gccgaagaag ttgtcacgca
     5341 gcgcctgagt gcccacgagc tggccacagt aggcctgggc gagctgcgag atcgccgtct
     5401 gctcggagga cacgaacgcg gtcacgagcg gcccggcagg catcgactgc tgctcggtgt
     5461 tgtagagtgc cgcaaccgtc gcattggtga acggcacgcc cgtgatgttc gacatcgcgt
     5521 gatagatcct ctcgaacgtg gcgacgccga cgtccggctg cggcgtagtg ctcggtaccg
     5581 gcggctgcag cgtgtaggtc ggctcgacga acacatgctg atgcgagccg aactggtcga
     5641 acgacaggaa gaacatgtcg ctggccatgc cggcatccac cgccagcacc gccccgacat
     5701 tcgacagcag ctgaccgttg gcggccgtgt agcccggcgg cccgactgtc gtgctcagcg
     5761 tcgagtacga ctgacccacg gtcgggaccg agccgttcat gccgatgcgc aggcccttga
     5821 tgacgaggct cgagggcgca gccttcgagt tcaggctgaa gaaggtcggc ttgttgaaca
     5881 gatagctgta ggagtcgtac tggctcgcct gcatgaggat gtagctctgg gcaatgccgg
     5941 agagcgagct cacgtcgaac agcaggtagt agctctgccc cacgccagcg gcgaagttct
     6001 gctggatctg cgtgggggtg agcgcgcgac tgtggatcgc cgcgaacttg atgaggccgg
     6061 tccactgctc cttgccggtg acttcacttc ccagaaccag ggcgaaagtg ttgtcccagt
     6121 tcgagagtgc ggcgcccttg ttggggtcga catcgcccgt gaacactccg ttcacataca
     6181 gtttctgacc gttgatcgga tcgtaggtca gcacgatgtg ctgcagcgcc gcctgcgcat
     6241 tcatgttcgc cgcggcggtc agcagcggcg gcgagccatt gctgtcggtc acggtgctgc
     6301 gcgtcatgcc ctcgtactgc tgcatgtgct gcgccagtgt catgtcgcgc gtcttgtcgc
     6361 tgccggagta cgagaggatg tacgcatccg tctgcgtcac gttggccggt gcggcccata
     6421 cttcgatgga gtactcgccg gtcgcctgga tcagctgcgc gatcttctgg ctcgaggagg
     6481 tagaggcctg cgccttgcct cccatcggca cgttgatgcc ccagccgccc gcccaggtca
     6541 cgggtccgta gagagtgagg tcggccgagg ggctcacacc gctcgtatcg taggcggtgt
     6601 ttccggtgcc ggtctcgaac atgtacttcg ccacgatgtt ggcctcataa cggcttccgc
     6661 cggcggcgat ggtgccctgc tccatcgtga gcgccttcga gatcacgagc gagggatcga
     6721 cctgtgtcgt cgggatcgtg ttggcgtagg cggtgatcgc cgcgagcatc tttgcggcgc
     6781 tgcccggaca gtccggcggt ccgcctggcg tcgtggccca gcagtgatgc agctcgctct
     6841 gcaggcgctc cacgaagcgc gactccgagg gtacgtcgag attgatcttg gcctgcgcag
     6901 cgagataggc ctggttcgga tcgctgctgg cgaagtacgg cgcctgcgcg gtgctcgagt
     6961 cgggacgatg gcagttgctg cagaactgcg tgaggatcgg ctgccagatg agctgctgga
     7021 aggcggtcga atccgcgggg aacagcttgc tgcctccgac cgactgcacc ggcggcggcg
     7081 tcagggtgat cttggtggcg acggccgagc cgcccccaag ccagttgtcg atccaggtca
     7141 gcatcgtctg ggcgcaagcg ttcgggtcgg cgacccagca gttatggcca ctgccgacgc
     7201 gcagcaccag cgtggactgc gagggattgc ttgcatccac cagcggcaga gccgcctggt
     7261 atgcgaggtt tacgtcgtcc gagcgcgcaa acatcggcga ctggccaccc tcgtggtggc
     7321 agccgccgca gcggttggcg acgcggatgt tctgccagag gttgatctga aaggcctgga
     7381 tgtccgctgt ctgcggcgcc gggccggtat aggcgtttgc cgtcgtggtg ttgctggtca
     7441 cctgattgac cgcggtcggc gctccgcccc cggtgcaggc ggcgagcact gcagccagcg
     7501 ccagcgctgc cgcgacccgc agagttccaa cacctgcgaa cgtcggttcg gtacggtaca
     7561 tgagatgctt ccgtttcatt gcggctctct cttaagtgcc cgggcaggcc gcggcagcct
     7621 gctggaacac ctgcttcagg tggtaaccgc ccgctttgaa gctggccttg atgctgccga
     7681 cggttgcgat gtccgcggca ttggtgggcg cgcggaagca tacggtctgg aacaccttcg
     7741 tgacctgaca ctgggcgaac gcatcggtcg actcgatctc ctggcccagc gatttggcgc
     7801 cactgccgga tccgggcagg ctggaatccc agccgagcac ggagttcggc ccggcgcgcc
     7861 agcggttgga ccagctgtca tcgggcgtga cgaagccctg actgaagttc tgcgcattga
     7921 tcaggtattt gggctgcacc ttgcccggcg tgtagaccag ggctccctgc gtgccgaccg
     7981 tcgcatcgaa gttgtagtag gcgaaggcct gcgccatggg atccatgccg ctgtggcagc
     8041 cgacgcagtt gttgaggaag gtgcgactgt caccgcccgg tgagcgcgcc acatcctgcc
     8101 ggattcgatc cgtgggccgc gacgtgtcca tcagggtggg catgtcgttg cacagatgat
     8161 tgatcatcgt gaatcggaac atcgcgcggt tcgtgccatt gatgaagaac gccgacgttg
     8221 cgccgcgcgt ggtgagcagt cctgcggtgg ccgccgtcgg aatgccatag gtggccgatt
     8281 gggtggtggc ccgcagcgtg gagctgagat cgacgccgtt cgtctcggcc atcgcatagt
     8341 gatcgttgtt ggataccgag ggggccggca gcccgctcgc gttcaccgta tagagaatgt
     8401 ccgccgacag ggccgtgttg aacggcacgt cgtcgtgcac catgccgatc accgtcgcgg
     8461 catagtcatt caacggtgcg aacaccgtgc cgtcgcggtt ggtccagggg atcaccatgt
     8521 tcttcaacgt gatgttgtag aacgttggcg cgctggtcgc gatggcggcg gcttgcatca
     8581 acccggtctg tcccggattg ttggtaatgg cctgcgccat cgatgccagc acggcggggg
     8641 ttggaggcac gccggcgatg cgctcataga tgcgctctgc ctgctcgttc gggcctgcga
     8701 aggccggcgc ggccagctgg gtgaggacgg cgagcatgca cgcgccgctc gccgcacgcg
     8761 acagccgcgc ggcggggtgc ttgccgctgt ggaatctggt gctgttcatg gccatggcgc
     8821 tcgtttactc ccagtacaat gccgatgcgc ggtcaggact ataaccgtcg tccaactcgt
     8881 ccccgaatgt cggaattcca tgacgtcgcc gggtacagac agttttgctg gcggttgagc
     8941 cgcgtgatgc aggacgaggc aaactgcgag tccgatcacg gcgttccgcc gatcgaaggt
     9001 ctacctagta gctcctcgat cgtcagcggg ctacgtccgt tgcagggaac atcgaaacga
     9061 gcccgcatgc cgtgatgcag tcggcataac gcaatagctg accgtgcggg cggaaaatta
     9121 ggttacattg cgccgagctc gcgcagccgt ggatgggtcg atgtggagag ggtgggaatg
     9181 aacgtcaatg ccggaatcgt gacagagatc atgtcgcggg tggtgcagcc gggccgcgct
     9241 gccagcgcgc agcgcctgtt gcccgcagtt ctggccgctg tgctgctcgc gctcctgagc
     9301 ggctgcagcg gcaatgcgca catcgacgtc gccaacagcc aggtcgccga cccggccacc
     9361 gtcgatttcc ccatcttcta tgtcaagcgc acgattccgg ccaagccgga cgatctgcgc
     9421 gtgatgcgtg atctggttcc cactgccgac ctgttcaagc gcgatagcgc ctcaccgagc
     9481 gcgaatgaga ccaacatcac atcgcgcgtg accgccaacg ccgcttacga cgtgaaggat
     9541 gtggacgtct cggccgacgg gacccgggtg gcctttgcga tgcgcggtcc catcacgatg
     9601 aacatgaaag ccaaggaccc gccatcctgg cgcatctggg aatacgtcat tgccacggac
     9661 ctgctgcacc aggtcatcga tccgaccacc gatcccgatc cgccgacggt gaacgacgag
     9721 tcaccgcatt acctgccgga cggccgtatc gtgttctctt ccacccgcca gactcagtcc
     9781 cagggtgtcc tgctggatga gggcaagccg cagttcgagg cgcaggatga ggaccgtacc
     9841 gagtcgacat tcaacctgca cgtgatgaac cccgacggct cggacgttca tcagatcacc
     9901 ttcaatcaga gtcacgatcg cgatgccacg gtgctctcga gcggccgtgt actgtggaca
     9961 cgatgggacc atgcacccgg ccatgacgcg atgcacctgt actccgccaa tcctgatggc
    10021 acggacctgg agctgtacta cggcgccaac agccacatga cgggcaccaa caatacggtg
    10081 atcgaattcg tcagggcgcg cgagatgcag aatggcgaca tcctcacact cacccggcca
    10141 tacacggacg cctcggcgac ccagaagatc gacttcggcg gcaatctcac gatcatcaat
    10201 ggcaaagact tcgtcgagaa cacacagccg ctcctggcca acgccggcct aaagggaccc
    10261 gccgagacac ccgcaacgcc caacaatgtg tccaccatcc ccgggccctc gccgggcgga
    10321 cgattcgatt cggccttccc gctctgggac ggcaccagcc ggatcctcgt gagctggtcg
    10381 caatgccgcc tcctgaacac cgccaacacg atagtgccct gcactgcttc ggctctcgcc
    10441 gaccccaacg tgatgatggc gccgcccctt tacagcgtct ggatgttcga ccccaaccag
    10501 aacaccatgc tgcccgtgat gccgccggta gaaggggtga tggtgacgga tgtgatcgct
    10561 gcccagccgc gcgcactgcc gaacgtcatt ctcgacaaga tcccgggagt cgatctggat
    10621 caggacctcg tctcggccgg ggtcggggtg ctcgacatcc gcagcgtgta tgacttcgac
    10681 ggtgtcgaca ccgcgaagcc caatatcccg acggtcgccg atccggccaa gacacccgcc
    10741 agtcagcggc tggcgcgctt catccgcctc gaaaaggcag tctcgattcc cgaccggacg
    10801 ctggtgaagc tgtcgggcgc cgccttcggc gccagcaact acatgcgcga gatcatgggc
    10861 tacgccccgg tggagcccga cggctcggtc cacatcgagg tcccggcaaa cgtcgctttc
    10921 atgatgagcg ttctggatgc caacggccgc cgcatcggac ccgagcaggg ggtctggctg
    10981 caggggctcc cgggtgaagt catttcctgc aacggctgcc acgtgccggc aacaccgcag
    11041 caccccatat cgcacggccg cgccggtctg ttcaattcga tctacgccgg cgcgatgacg
    11101 accggtacgg cctttcctca cacgatcgcc accggccccg gcgccttcct gcccaacgcg
    11161 ggtgaaacga tggcgcaggc gcgcatgcgc accagctgca cgaacgactc gcccagatgc
    11221 gcgcaaatgg tgcccagcgt gaacgtgctg tacacggacg tctggactaa ccccgcgcaa
    11281 gcaacgccag gcaagccgat cacctaccgc tacgacgatc cgaccttcat gactccgatc
    11341 ccgacgctcg ccgcctgcgt cggcacatgg gctgctaact gccggatcgt catcaattat
    11401 ccgcagcaca ttcaggcgct gtgggatctg acgcggcaaa cggtcgtggc aggggtcgtc
    11461 acggcggatc acacctgctc gcaggcgggc tgtcacaatc cgaagaatgc cgccggcgtg
    11521 gccgaggtgc cggccgggca gctcgacctc accaatgccg cctcgaacgc cgagccgcag
    11581 cagttcgtct cttaccagga actcctgttc ccgcacgcgg agcaagaagt taacatgggc
    11641 gccttgcagg atgtcccagg gccgcccggt gccaacggcg ccccgacgac catccctgtg
    11701 gggccgttca tgaatgcggg tagcgcgaat ggggcgctct cgaatgcgtt cctcggccgc
    11761 ttcgcaccgg gctcgggcag cacccatgcg ggttacctga gtccggccga gcttcgcctg
    11821 atctcggagt ggctggatat tggcgcgcag tacttcaaca atccgttcga cccggccgta
    11881 cccgtcaact gagccggcag cggatatcgg gcactgcacg agcgacagac tctccggtaa
    11941 gtcatcattg cgaactctcc tcttcgtcgg actgctgctg tcgctgtgcc tgcccgcgcc
    12001 cgccttcagc cgcgagcgcg agcacctgca ggtcttcatc acggccccgt atctggagct
    12061 gcgtaccggc ccgggacgcg gctatccggt tttccatatc gccgagcgcg acgaaagcgt
    12121 ggaggtgctc ttccggcgca ccgactggtt caaggtgcgc accgagcggg gcgtggaagg
    12181 ttgggcagcc cagcgcgaca tgctcaggac cgtgctggcc gatggcacgc cgttccgatt
    12241 cgagctcggc gatcgctccg ggttcacctc gcaccgcttc gagatgggca tcttcgcagg
    12301 cgattacggc ggcgcgaccc tgatctccgg ctacagctcg ttctcgttca actcccagct
    12361 cgccgcggag gcctcgttcg ggcagttcct gggcaagttc accaacggag tggtcgccga
    12421 tttcggcttc tcgcacgtgc tggtgcccga gtggcggctg tcgccattct tcatgctcgg
    12481 caccggtctg gtgcacgtct cgccgaaagc caccctcgtg gtaccttccg agcgcaccga
    12541 tcagactgcc tacgtcggcg gaggcctgcg cttctacctc acgcgccgct ttttcctgcg
    12601 cgccgagtac aagagccaca tcgtattcac gaagcgcaac gcaaatgagg aagtagacga
    12661 atggaaactg ggattcgcat tcttcttctg actgtggtcc tggtgggact gccgggttgc
    12721 gcttcggtgc gcaactggtt ccaccaccgc agcgaggtgc gccaggcgcg ggccgaggcc
    12781 cgcgaagagg ccgcccaggc ggatgcccag gccgcccagc aggactcgga ggcctctccg
    12841 cctcgggtga tcgagccgga ggtccaaaga cgcaagatca aggtgccccg gatccacagc
    12901 tcgaacgtgg agctcggcct gcagtacggt ttcatttcca tcgaggactt cggcacccag
    12961 tccacttacg gattgtcggc ggcctaccac gtcaccgagg acttcttctt tctcggggag
    13021 gcggggcgcg cctacgcggg ccggaccagc ttcgagaccc tggggggcaa tattcaactg
    13081 cttaccagcg ctgagaggcg cttcacgtat tacgatctct cgctcggtta caacttcctg
    13141 cctggcgaag cctttctcgg tcgcggcgtc gccttgtcga gcgctttcta tctggtaggc
    13201 gggattggca gcacacagtt cgcgggcgat gatcggttca cggtgaattt cggggccggc
    13261 taccgggtgc tgccggctga ctggctcgcg gtgcacatcc aggtgcagga tcgcgtcttc
    13321 aggaccgatc tgctgggagt gtcgaagctg accaacaaca tggaagcgac catcggagcg
    13381 accgtcttct tttgagcaca ggaactgcgc acatgaaaaa gtcgacccac gcggcaaagg
    13441 gtgcaaccct tgccatgttc gccttggcgc tgctggctgt cgtgcccgcg atgggcgcgg
    13501 atccaagcgg gccggcgccg gccttcacgc tggcccaacg tggcggcggc aacctgagtc
    13561 tcgccaagta caagggtcag gtggtgatgc tcaacttctg ggcgagctgg tgtgggccct
    13621 gccgccagga gatgccgctg ctggaggaca tctacaaaaa gtatggcaag ctcggcttca
    13681 ccctcatcgg ggtgaatgtg gagccggact cgaatgcggc caatgactgg ctgaagcaga
    13741 cgccggtcag cttccccatc gcctacgaca cgcagagcca ggtcagcaag ctctacgacg
    13801 tgcaaggcat gccgagcacc gtgatcatcg accgcaaggg caatcttcgc gtgctgcacc
    13861 gaggctacaa gcccggtgac gagaacggct acctcgacag catccgcgcc ttgattcgcg
    13921 aataggctgg ccgagagccc ggagcagcgc cgatgagcag tactttcaac acgctcgccc
    13981 agaccgcgcg gctcgtggcg caagccatcc cccggcgacc cggactgccc cggctcgctg
    14041 cgctcgggct gatcgccatg gcgctcgagg tcgcgctctc gggctgctcg cccatcgagc
    14101 cctgggtcaa accctacgag cgcgagcact tcgccgatcc gatcatggcc ttcaaccgtg
    14161 atccggtctc gagtgtgtac cgcgagcacg tgttcgagtc gcgcgagggt gcgcggggcg
    14221 caacgggcgg cgtgggtggc ggctgcggat gcaactgagc ggcgcaccgg ggcggctcga
    14281 ggcggcgttg cgccggcact ccggcacgct gctcgcattg tgcctgtgcg tggctccggc
    14341 gctcgccgga gtgctgcccg aggatcggac cgatctgctg taccaccgtt atgaaggcgg
    14401 cggcatcacg gttcagggcc cttcgctcct catccagaag aagctcaccg acaacctggc
    14461 cctcaccgcg aactactacg aggacatgat ctcgagcgcc tcgatcgacg tgaagctcgc
    14521 ggcgagcccc tacaaggaga cgcgcaagca gaagagcgcg ggcttcgagt atctgcacgg
    14581 caagtcgacc tatagcgcgg gcctgatcaa cagcgtcgag ccggactatc gctcgaacac
    14641 ctcgtactac tcgatcagcc aggacatgtt cggcgacctg accaccttca cgctcaccta
    14701 caagcgcggc tgggacaagg tcttcaagga cggcaaggtc gccggccgga tcgagaacgt
    14761 ccctttcccc gacgaggccg gggtgcctga cccgggtgcc ctgagtcagg atgccgatca
    14821 tcgcggctac accgtcggcc tgagccagat cctgacacgc aatctgatcg cgaccctcaa
    14881 ctacgaggtc ctcactgacc agggatactt gcagagcccc taccgcgaga ttctgttcct
    14941 cgatccgacc caggcgctcg gctacagcaa ggcggatcag atctatccca acacgcgcac
    15001 cagcaatgcc gctgccttcg aggcgaagta ctacctgccg taccgtgcgg cgctcaccgg
    15061 ccagtaccgc tacttccagg atacctgggg gatccgcgct cacaccttcg aggtcggcta
    15121 cacacagcct gtgtggcgtc gactgatcct cgatgggtcc gtgcgcttct acacgcagaa
    15181 cgctgcgagc ttctacagcg acctcttccc gcgggccgcc ttccagaact tcgaggcgcg
    15241 ggaccgcgag ctggcggcct tcaacagtta ctcgctgggg ctcggcgcct cgtgggactt
    15301 ccccaagccg ccggtgccgt ggatcaatca cagcagcctg agcatgcgta tcgatcatct
    15361 gctcatcgac tacaaggatt atcgtaacgc gctgctgatc gatccggccg ccggcatcag
    15421 gccgggcgat gagccgttgt acaagctgaa cgcaaacatc ttccagctgt tcatgtcggt
    15481 ctggttctga caatccgtcc cccctgcggt cttgagctga gttaagcttg cgcaatcggt
    15541 cgcctgcgct gtcggcagct ggcgacagct cagaggattc tcagcatgcg aagtcgcacc
    15601 agattgttca ccgttctcac agccctcgtg ctgtgcgccg gcgcccaggg agcttgggcg
    15661 caagcacggg aagaaggacg tctgctcatg gcgagccagg tgctcgatga gctgcgcaac
    15721 gggcgggacc aatacgttcc ggagaagctc ctcgagcgcg cctacgccat cgccatcatt
    15781 cccgacatga cgaagatcgc cttcttcgcc ggcggcaggc gcggtcatgg cgtgctcgtc
    15841 gtccgcgaca agaacgggcg cttcaccagc cccgtattca tcaccatgac gggcggcagc
    15901 ttcggctggc agtggggtgt gcagtcgacg gacatcatgc tcgtcttcac cacgcgcagg
    15961 ggggtcgaag ggatcaccgg tggcaagatg accctgggcg ccgacgcttc ggttgccgcc
    16021 ggtccagtcg gacgcagcgc ttcggcctcc accgacacga gcttcaaggc ggaggtgtat
    16081 tcgtactctc gcagccgagg cgtgttcgcg ggcctggcgc tcgatggcac ggcactcacg
    16141 atcgatgacg acgcgaacgc cgcgttctat cgcaagcccg gtgtcatggc ctcggacatc
    16201 atcgcaggga acattcggag cgatgacgaa agcgcccggc gtttcctcgg tgccgtgaat
    16261 aacagcaccc agtctgaatc agcctcctcc gccactccgg cgaccgcctc cgctcccacc
    16321 cccgagctgc cgcagggggc gccgcgcagc gatgcgccgg cacctcgatc gcagtccagt
    16381 ggcgcgcaga gtttcccgct ggaggatccg aagccgggag ccgagccgaa atagcgcgta
    16441 cgggtgagcg gcgcgtacgg gtgaactgct tgcgtgtggc gcggcgccct agcgccggtg
    16501 accgctttca gggcgcaagc cacacgcaag cagctcactc ccttgcgtct gagcccgctt
    16561 ccagggggtg acctcccacg caactagccc tccaccgtgt agggtaggcg catgcgcgat
    16621 ctcacccagg gcccgatcag caggcaactc ctcgccatgg ccgtgccgat cgcggccggc
    16681 atgatcttcc agacgcttta tttcctggtt gatctgtatt tcgtcgccgg gcagggtgat
    16741 acggccctgg cgggcgtgag ctccgccggc aacgtctcct tcgtgatcct ggccctgact
    16801 cagatgctcg gggtcgggac cgtcgcgctg gtctcacacg ccgcgggccg caaggatcag
    16861 gcggaggcaa acctggtctt caaccagtcg gtttgtctct cggccctcct cgcatgcgtc
    16921 acgctcatcg ccggctattc gctcgccccg gcctacgtgc gttcagtcgc ggccgaccct
    16981 cactcggctg cagccggaat cacctatctg cgctggtatc tcccaggtct cgctcttcaa
    17041 tttgcgctgg ttgcgatggg ttccgctctg cgcggcacgg gcatcgtgca gccgacgatg
    17101 atcgtgcagg tcctgacggt ggtgctgaac accgtgttcg cacccattct catcgccggc
    17161 tggggcaccc atcaccccct cggagtcgcc ggcgccggcc tcgccagcac gcttgcgatt
    17221 gcactcgggg tcctcttcct ctggctgtat ttcgcccggc tcgagaagta tgtcgccctg
    17281 catcccccgc agtggctgcc gcacccgggc acgtggcgac ggatcctgaa catcggtctg
    17341 cccgcaggcg gtgagttcct gctgatgttc gtcttcatcg gcatcgtcta ttggacgatc
    17401 cgccacttcg gaccgacagc ccaggcaggt tttggcatcg gctcgcgagt gatgcaggcc
    17461 atcttcctgc cggcaatggc gctcgccttc gccgcctctc cagtcgcggg ccagaacttc
    17521 ggggcggccc gcatggatcg ggtacgcgcc acgtttcgct cggccgcttt catgagctcg
    17581 acagtgatgc tggcgctgac actcttctgc cagtggcgac cggatctgct gattcaggcc
    17641 ttcagcagcg acccggccgt cgtgggggta ggcgcaccct acttgcgcat catctcgtgg
    17701 aacttcgtgg ccacgggtct gatcttcacc tgctcgagcc tcttccaggc gctcggcaac
    17761 acctggccct cgctggtgag ctcggcgacg agactcgtca cgtttgcact gccggccgtc
    17821 tggctgggat cccagggcca cttcgagctg cgccacctgt ggtatctgtc ggtgtgcacc
    17881 gtctccgttc aggccgtgat cagcctctgc ctgcttcaac gtcagtttcg ccagaggctg
    17941 gtgcaaagca caccggcggt tgccggctga tccagccgct accttccaga ccagtggcgc
    18001 cagtcctcga gcgtcgcatc gcgcatccgc cgcgccgcct tgagcagcca gtcaggccgg
    18061 cggcgtgcga gatcccgcag gatccctttc cgctggcgcg gagtgcccag atgcacattg
    18121 gcggtctccc aacccatcat ccacaggagc cggcactcgt cgcggatccc ggccacgctc
    18181 tgcagctcga tgcggctgca gtccggggcc aggcgtcgga tcacccaacc gtcgcgcacg
    18241 ctgaggaaag ggtccggaac gcggacggcc cgccggatga cgtcggaata ttggagacga
    18301 ggctcgcggc tggcgctcat ccatgaccac gccgagctcg tgagcggttt ggtttcccgg
    18361 gcaatcgtgc cgccgcgcca atcggcgaga gccgtatagc gctcccggcc gaggctcccc
    18421 aggcccgcct gccgatgcac gatccggaaa gcgatatctc gttccggcag ggcgttgcgc
    18481 aagatggctc gcactcgcgc cggcacgacc gcggtgaccg gtcgcaggct cagcaggcga
    18541 tcccagtacg ttcgctgctc cttcagacgg ctcaccgcca ggtcccgcag ccagcggtgt
    18601 cgctccgcga gcacgaccgg cgcgccttcc tccttcaggg atcgcacgta gccctccaaa
    18661 acggattcgc acgccaggcg agccggcgta gccaggcggg attccccgat cgcgagcagc
    18721 gcgctggtgc aaaggcgcac cagatcgttg gcatagggaa gcgtcgtcgc ctcgtcgaaa
    18781 tcgtttattc cccagatgag gcggccttct ccatcacgcc aagtgccgaa attctccacg
    18841 tgcaggtcgc ctatcccgag cacccgcggc gccagacaca ggctggcgca ttgcagcggc
    18901 cacaattgcg cccagcgata gaaggaagcg cgcatgaaaa agaacgggtc ctgctgcatg
    18961 cgctgatgtt tgagccgcag atccggcttg aggatcttca ggcgcttggc cagccagcgc
    19021 tcgtaggccc gggtggactc gaccgcagtc atctgctcgg gcctgctcat cacggcgcat
    19081 tatccgctcg gctcatcacg gcgtgctccg ctcctgcgcc gccgcttgcg gactgggccc
    19141 tctcgcatca tcgcgcgagc gcgcgatcca ggcgttcacc tgcttctcga gaatgcccag
    19201 cggaagcggg ccgttgccga gcacgacccg atggaactgc ctgatgtcga aacgcggccc
    19261 cagccgctcc tgggcctgcg ctcgcagctg cagaaacttc agcatgccga tcttgtaggc
    19321 cagcgcttgc gcgggaacga catagtagcg ctcgacctcg ctcacaacct cgccttccgc
    19381 catgccggtc ttctccatcg tgtaggcgat ggcctgctcg cgcgtccagt gcatgcgatg
    19441 aatcccggta tcgacgacca ggcgcacggc ccggaacatt tccgcctgca ggcgtcccag
    19501 gttgtcgagg ggatcgcgct cgaaccccat ctcccaggcc agccgctcag cgtaaaccgc
    19561 ccagccttca tcgtaggcgg tgaacggcag caccttgcga aacaccggga catcggtgag
    19621 gcgctgcgcg atggcgccct gaagctcgtg gccggggacc gcctcgtgat aggcgagcgt
    19681 gcgcatctgg tatttccaga tctcgcgcat atcgcgcaga ttgacgtaga acactccaac
    19741 tcgagagccg tccagcgagc ccggatccgc gtaggcaccg ggcgcggtct tctccttgaa
    19801 gggcggcaca cgctccacat ggatgtcgag cgctggcggc gcggcgaact cgcggggcag
    19861 atcgcggatg atctcgttga tgtaacgttg atagtcggcg aggcattgcg cccgcccgtg
    19921 gtcgtcgttg gggtactgga agcgggaatc ggaatcgagg accgctatgc gtgctccgat
    19981 ggtgccgtgc tcgaggcctt gcgatctcag aatggcatcc agctgcgtct cgatgcgcgc
    20041 cacctccttc aggccgatct catgcacggc ttcggcattg agcgcggtcg tcgtatggct
    20101 tcggatcagg tagtcgtaat acgcatcgcc gtccggcagt ttccacaccc catagtccgg
    20161 cgccaccttg ggatacagat gctcgaaata ggcgatcaaa ctgcggtagg ccggatagac
    20221 cgtctgttcg atcgctgcgc gcgcctgatc ctgcagccgg gcgcgttcgc tcgcagacag
    20281 gtttccaagc ttggccagct tgtcgtgcag gttgacgtac aggacattcg cgctcggcgg
    20341 cactgcgatg aaggcccgca tctcggccag cacccgcgcg atgacgaagc cgggcggcac
    20401 gatgcccttg gactcccgca gttgcagccc ttgcagcaac ccgcggaact ggcgtccgaa
    20461 gtccgacagc cgcgagacgt agttgcgcgc atcgcgctga tcggcgagcg gatgaatctg
    20521 caccatgaag tccggggttg cgctctggac gccgaagagc tggttgaccg ggtagtcgta
    20581 gtagagccag cgttgcccgt tcacctgatc ctgcaggaac cattcgagca cgtcgtacga
    20641 gagctgctgg gaagcggaga gcgagctgcg atcgtactgg tgcagggtcg cgagatcgtc
    20701 gcgaaccatc ttcgcaagct tctcctcgtg cgccggcgac acatcggtga ggctgccgtc
    20761 gaatcccctg tagccgagct gctcgaagat cccgagcgag gaggccgcct ccggatcctc
    20821 gaggaggaac tggatgagca cccgctcgaa gaagatgccg atcgtgagcg gccggaagta
    20881 ccagctctgc agaatgaggg cgaagcagag cagcgcgacg gctccgatca acacccccag
    20941 ccatttgagc gcggtcctca ttcgaggacg ggaggcggcg ctactgcgtc cgggcagccg
    21001 cgcgatcgag gatgagctgc gcaaggggct cgtagttgcc accgaagtga tggccgcccg
    21061 ggagctcgac gacgcttgcg tgctgagcgc tcaccttcgg gcagatcgta tccgtgtcgt
    21121 cgtcgccgta gatgcacaca gtgtccgcgg aggaaagctt gtccatctcc ggcgggacgg
    21181 gcagtccgcc gtcctcggca ccgacccagt tcgccacatg gaactcgaat gcggccgagt
    21241 cgctgagccc gatcagagcg gtgagctcga tcagggcccg cgtcggcgca ggcaggcgat
    21301 tcacagcaaa tggcagcaca tccgcgcctt gggaatatcc gatcaggatg gcgtgtttct
    21361 tcttccagtg aaaggcatag tagcgcacga tccgatcgat atcctgggcg agaccggcgg
    21421 gcgtgcgcac tgtccagaaa taacgcagcg agtccacgcc ggcggtcggc acgccatgct
    21481 cggcgagcag ccccgcgacc tgcttgtcga tccccgccca gccgccatcg ccggaaatca
    21541 acacggcgaa cgaatcggca ttgcccggtg ccggcgttcc ggtcgcttcg acctcgacga
    21601 tgggcaggtc ggcgagactt gccggcggca ccggcaggcg ctgcacctgc gtgcttgcaa
    21661 tggaggcgta cgctgcatcg aactgggaca gccagccctt cgtaccggag tagttgtggg
    21721 tcactcccgg cagctcgatg agtttcgcac cgggggttcg tgccacgaag gctcgcgcac
    21781 gcgagatcgg gcagacccgg tcgcgcgttc catgcagcgc tatccaggga gcgtgcagct
    21841 gcgaaggagg aagcagctgc gcaccggctc catgtgcaag cggcttgaac cgcaggcctt
    21901 gcgtctggca cagaggcttg cgcaggtcca gatcgacgca gaacgaaagc gtcacggcgc
    21961 ccgcaaaaat accggggggc gcctgcgcca gcgtcgtgta cgccagcgtc gcccccgccg
    22021 aatggccgac caggatgggc gtgaaatagg tcggcagctt gtaataggcc tggatgtagt
    22081 ggctgaggtt ttccagatcg ccatcggggg ccacgcagct gccgccgtct ttctccaggc
    22141 tggtgaacag ctccgcggta tcgatgcccg cgaccatggt gccttgcgcg gcgagcgatt
    22201 cggcga
//
LOCUS       DASRHN010000400         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1304886, whole genome shotgun sequence.
ACCESSION   DASRHN010000400 DASRHN010000000
VERSION     DASRHN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304886"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2094
                     /gene="lon"
                     /locus_tag="VFB37_16235"
     CDS             <1..2094
                     /gene="lon"
                     /locus_tag="VFB37_16235"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZO24046.1"
                     /translation="RFRAVEFVQEKPYLAARTEVLAPANTTGPEIEARVEVLKQRAIE
                     ALSLLPQVPTELTRTIQSIDSASQLADLIVSFMDVKVQDKQEMLETLDLRERLDKVLT
                     LLSNRLQVLKITRDITEQTQSALGERQREAVLREQMRQLQRELGESEDSGSEVAELEK
                     AVERAGMPAEVAEHTRKELKRLQRMSEASPEYGMVRSYLDTLVALPWSTADAEDIDIE
                     RARRILDEDHYGLDKVKRRILEYLAVRKLNPQGRSPILCFVGPPGVGKTSLGQSIARA
                     LGLKFVRASLGGVHDEAEIRGHRRTYIGALPGNIIQGLRKAGTRNPVFMLDEIDKLNA
                     SYQGDPASALLEVLDPEQNSTFRDNYVGQPFDLSRVLFIATANVLETIPGPLRDRMEI
                     IELTGYTEEEKLQIARRYLVQRQLKANGLEPSQATITDAAIARVIREYTRESGCRNLE
                     RDIGALLRRAAMRIAEGKVTSVQIDAADVTDILGPTRFENEVAQRTSLPGVATGLAWT
                     PVGGDILFVESARLPGSGKLILTGQLGDVMKESAQAALSLVKAQATEFGIDAQQFDRI
                     DIHVHVPAGAIPKDGPSAGVAMFVSLASLLKNRAVRPDVAMTGEISLRGLVLPVGGIK
                     EKMIAAARAGIRKVILPARNRRDLEDIPQSARSLLEFVWVEHVSEALEVALGQGKPAD
                     KTGTVRNERPEMSTA"
     gene            2212..2685
                     /locus_tag="VFB37_16240"
     CDS             2212..2685
                     /locus_tag="VFB37_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24047.1"
                     /translation="MSAVLLAVFNDYDTADRVRTDLVRDGFPTDRIDLTASHEPGRAG
                     LQPGPAVHDRYVQYFRTLFGREDERSCVERLVERIEGGAATVTVHPRGAIEAARAMQI
                     LDHGVPTEKVRHDLDNAGMEHAAASHEGAWIRNFWVESNPEYHCIYCRMFPAHKH"
     gene            complement(2689..>2934)
                     /locus_tag="VFB37_16245"
     CDS             complement(2689..>2934)
                     /locus_tag="VFB37_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24048.1"
                     /translation="NIDLACTPAESGSEPVSHESVATVAENPIARERIAVAKTSTPAE
                     RSAVADTEVVVETAAVVETAPLAESAAPVPSGKTAFG"
BASE COUNT          534 a          905 c         1001 g          494 t
ORIGIN      
        1 cgtttccgcg ccgtggaatt cgtgcaggag aagccctatc tggctgcccg caccgaagtc
       61 cttgcgccgg cgaacaccac cgggccggaa atcgaggcgc gcgtggaggt tctcaagcag
      121 cgcgccatcg aggccctgtc cttgctgccg caggtcccga ccgagctcac ccggaccatt
      181 cagtcgatcg actccgcgag ccagctggcc gatctgatcg tcagcttcat ggacgtgaag
      241 gtgcaggaca aacaggagat gctcgagact ctcgacctgc gcgagcggct ggacaaggtc
      301 ctgacgctcc tctccaaccg gctgcaggtt ctgaagatca cgcgcgatat caccgagcag
      361 actcagagcg cgctcggcga gcgacagcgt gaggcggtgc tgcgcgagca gatgcgtcag
      421 ctgcaaagag agctcggcga gtccgaggac agcggcagcg aggtcgccga gctggagaag
      481 gccgtggagc gtgctggcat gccggcggag gtggccgagc atacgcgcaa ggagctgaag
      541 cggctgcagc gaatgagcga agcgagcccc gagtacggca tggttcgcag ctacctcgat
      601 acattggtcg cgctcccctg gagcacggcc gatgccgagg acatcgacat cgagcgggcc
      661 cgccgcatcc tggacgagga tcattacggc ctcgacaagg tcaagcgtcg catcctcgaa
      721 tacctcgcgg tgcgcaagct gaatccccag gggcgcagcc caatcctgtg tttcgtggga
      781 ccgccggggg tagggaagac gtctctcggt cagagcattg cgcgtgcgct cggattgaag
      841 ttcgtgcgcg cgagcctcgg cggggtgcac gatgaggcgg agatccgcgg ccatcgtcgc
      901 acctacatcg gagcattgcc gggcaacatc atccaggggc tgcgcaaggc cggcactcgc
      961 aacccggtgt tcatgctcga tgagatcgac aagctgaatg cgagctatca gggtgatccg
     1021 gcttcggcgc tgctcgaggt gctcgatccg gagcagaatt caacattccg cgacaattac
     1081 gtcggacagc ctttcgatct gagccgggtg ctgttcatcg cgaccgcgaa cgtgctggag
     1141 accattcctg gcccgctgcg ggaccggatg gagatcatcg agctgaccgg ttacaccgag
     1201 gaggagaagc tgcagattgc caggcgctat ctcgtccagc gtcaactgaa ggccaatggt
     1261 ctggagccat cacaggccac gatcaccgat gcggccatcg cccgcgtcat ccgcgagtac
     1321 acccgcgagt cgggctgccg caatctcgag cgggacatcg gtgctctgct gcgccgggcc
     1381 gccatgcgca ttgccgaagg caaggtcacc agcgttcaga tcgacgcggc ggacgtcacc
     1441 gacatcctgg gtcccacccg tttcgagaac gaggtggcgc agcgcacctc gctgccaggt
     1501 gttgccaccg gactggcgtg gacgccggtc ggcggagata tcctgttcgt tgaatcggca
     1561 cgtctgccgg gttccggcaa gctgatcctg acgggccagc tcggcgatgt catgaaggag
     1621 agcgcgcagg cggcgttgag tctggtgaag gcccaggcta cggagttcgg catcgatgcg
     1681 cagcagtttg accggatcga cattcacgtc cacgttcccg cgggggcgat ccccaaggat
     1741 ggcccaagcg ccggtgtggc gatgttcgtc tcgctcgcct cgttgctgaa gaaccgcgcc
     1801 gtacggcccg atgtcgccat gacaggagag atcagtctgc ggggcctcgt gctgccggtg
     1861 ggcggcatca aggaaaagat gatcgcggcg gcgcgcgccg ggatccgcaa ggtgatcctt
     1921 ccggcacgca acaggcgtga cctggaagac atcccccaga gcgcccgctc gctgctggag
     1981 tttgtgtggg tggagcacgt ctccgaggcg ctggaggttg cactggggca gggcaagcct
     2041 gcggacaaga ccgggacggt gaggaacgag cggcccgaga tgtctaccgc ctaagggctg
     2101 ggcgatcccc cggcgatacg ggcgattagc gcctgttgca agcgcgggcg tacactcagc
     2161 gcacgttttc ggcgagagca ctgccggtac cttgacggca aggaggtaga tatgagcgct
     2221 gtcctgctcg ctgtattcaa cgactacgac accgcagatc gtgtgcggac cgatctggtg
     2281 cgtgacggct ttcccacgga tcgcatcgac ctgacggcga gtcatgagcc ggggcgcgcg
     2341 ggcctgcagc ccgggccggc ggttcatgac cgctacgtgc agtacttccg gaccctcttt
     2401 ggacgggaag acgagcgatc ctgcgtggag cgcctggtgg agcgcatcga gggtggtgcc
     2461 gccacggtga ccgtgcatcc tcgcggtgcg atcgaagcgg cccgcgcgat gcagattctc
     2521 gatcacggcg tcccgacgga gaaagtgcgg cacgacctcg acaacgccgg catggagcat
     2581 gccgcagcca gccatgaggg cgcgtggatt cgcaacttct gggtggagag caaccccgag
     2641 tatcactgca tttactgccg gatgtttccg gcccataaac actgatcgtc agccgaacgc
     2701 cgtcttgccg gatgggacgg gtgcggcgct ctctgccagc ggcgcggttt ccacgaccgc
     2761 ggctgtctct accaccacct cggtgtctgc caccgcggaa cgttctgccg gcgtgctcgt
     2821 ctttgcgaca gcgatgcgct cacgcgcgat ggggttctcg gccacggtgg cgacagattc
     2881 gtgcgaaacc ggctcgctgc cggactctgc aggtgtgcag gcgaggtcaa tgtt
//
LOCUS       DASRHN010000401        17231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_312776, whole genome shotgun sequence.
ACCESSION   DASRHN010000401 DASRHN010000000
VERSION     DASRHN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312776"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFB37_16250"
     CDS             <1..287
                     /locus_tag="VFB37_16250"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZO24049.1"
                     /translation="FSLSELATLADVRGWTAVLIWNNQGYREIRTAMTQAGIEPEGVD
                     VRPPDFSHIARAYGHPHRRITSRESLRKSLQEFASRRGVLMLEVSADEFA"
     gene            315..1910
                     /locus_tag="VFB37_16255"
     CDS             315..1910
                     /locus_tag="VFB37_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO24050.1"
                     /translation="MQTASRYDVIIVGGGHNGLVCGAYLARAGRKVLVLERNRQVGGA
                     AVTLEFAPGFRASTFSYLMSLLHPKVMRDLELERFGLRILPATDMFTPLAGGNYFLFT
                     GDDERNVREIARFSERDARQYPRFKRYLDQIAAAARRLMLQTPIDPQDLSLRGLAGLA
                     SFVFENRKSLKEFYGLYDIMTMSAYDFLSEWFESDVLKAVYAYYSGIGSFQGPKTPGS
                     AYILLHHLMGEHAGAGGWGFVEGGMGKISETIAQSGLTHGMHILTEADVDHIESREGR
                     ATGVVCRDGREFHAKVVAANVSAKLLFTRMMNGAQLPEEFRWHVETFRTESAAAKLNI
                     ACERLPQFSAFNSELCGFEYPSYAHVAPDVAYLEQAYADAKQGWYSQRPFVTVTAPTT
                     VDRTLAPAGKHVLNLFCGHAPYTLRNGASWEDHRQKFAELVLDALEQFAPGFRKDIIA
                     MQVLVPPDIERLIGSPNGHIFHGELSIDQLFFKRPAPHFANYRTPIRALYLCGSTTHP
                     GGGVSGISGHNAAREIQKDWRRL"
     gene            1924..2817
                     /locus_tag="VFB37_16260"
     CDS             1924..2817
                     /locus_tag="VFB37_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO24051.1"
                     /translation="MRLKKRNLSKAVIASREWYARLGELCAGGFTTASMIGLADELVE
                     RLDVDSILILRYPRASSPQLLYGRPDHRHRANTVEDYLLGHYALDPFYLRSDYCSQRG
                     LISLRDVIEEDFASSEYYKVHYQSAGLIDEMCFCCSDGEGGHLNLSLSRTLGKSHFSA
                     AELAGARAIAPLVTAALSSTWRALCPDASSAPQVTSADGEHHRHIENVRLNFGRSVLT
                     EREYEILQHLLRGNSVDLIAQRLDIAVSTVKVHRKHIYSKLEINSQAEIFTLFLEVVA
                     GTKYEPGSDPLARYQRSSARA"
     gene            complement(2828..3502)
                     /locus_tag="VFB37_16265"
     CDS             complement(2828..3502)
                     /locus_tag="VFB37_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO24052.1"
                     /translation="MCATRSMMVGESAALLEGTSTPRSPRSGSRASGERTRIEILRAA
                     KRVLATHGYAHFTLRRVANEAGLTVGNLAYHYPSKRNLVRALIMLLIEEYRAQIVARM
                     RVAPSRSPVAFRSLVAWLMRDSASAQTSRLFREFWTIALRDPFIAKAVDRFYGEVQET
                     ASQHLRHNFPNLPPASCRAIVQLMGIISEGSNVLYGTARHPTAPLSKVTQLACSLLVL
                     AAQQSA"
     gene            complement(3589..4491)
                     /locus_tag="VFB37_16270"
     CDS             complement(3589..4491)
                     /locus_tag="VFB37_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO24053.1"
                     /translation="MNDLTAQMIAILRRNGRASYSEIARELGTNRDYVASRINPLIES
                     GKLSIVASVHPRVLGLTTSAHLSIKAAGNIQSVIEALEKFDAPVLISVVAGAFQIVVE
                     LQLRSLTELNREVLAIRAIKGVREVFVHLYERILSSFYAGTGPESLSQFDEVDINIVN
                     RLLHDGRASFAEIAQVVGLSVSGCRTRVQRLLESGVIQIGAIKQRADMTSDLVFGIGV
                     NAHDDCREAIEAIAANRGLEFIARTVGRFDLLATVEFDSLRDFNLLVSRLRSLPSVEY
                     CEQWLHVRIVRERYWRTLAQPNQQ"
     gene            4670..5974
                     /locus_tag="VFB37_16275"
     CDS             4670..5974
                     /locus_tag="VFB37_16275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO24054.1"
                     /translation="MRAAHHPGALHVSSHGLWQRTAPGMAELRGLSEDLTADVAVIGA
                     GYTGLSTALHLVENRSSVVVLEAADIGFGGSGRNVGLVNAGMWVMPDTVVQRLGSEFG
                     TRLLQLLSAAPDVVFNIISRYAIDCEARHAGTLHCGVGRKGLAELRQRAAQWQARSAP
                     VELLEAAETRRRVGSAAFSGALFDPRAGTIQPLAYARGLASAALAQGARIFVRSPATT
                     VEPSGSGWVVRSPSGSVRAEWIVVATDAYARGPWDIVRREQVHLPYFNFSLPPLERSL
                     RDSILPAGEGAWDTRSVLSSFRLDAAGRLIFGSVGALRGADGGVHRQWALRAMRRIFP
                     QLEGLQFETGWFGHIGMTSDNLPRFHVLAPRVIGFNGYNGRGIGPGTTFGRVLAAFAL
                     GQLREQDLPLPVSTPKRVAFRSVREAIYAQGSQMLHFAGARF"
     gene            6053..6490
                     /locus_tag="VFB37_16280"
     CDS             6053..6490
                     /locus_tag="VFB37_16280"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HZO24055.1"
                     /translation="MKVVSAHSRIHRALGVLTATALLLVTASAAAEDVKVSLTGSEET
                     PPVTTSATGSGTIHIHKDKSVTGTIKTKGIEGTAAHIHLGAPGQSGPPIITLTKAANG
                     AWTVPKGSKLTDEQYQSFKDGNLYVNVHSAEHKPGEIRAQLKP"
     gene            complement(6609..7571)
                     /locus_tag="VFB37_16285"
     CDS             complement(6609..7571)
                     /locus_tag="VFB37_16285"
                     /inference="COORDINATES: protein motif:HMM:NF004793.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZO24056.1"
                     /translation="MSSGPDRTNASFIPAAVIRRLTPMRGLVPALREAFRRAEAMPTR
                     QRFQLPGNSTLLLMPAWRDGGDAGVKIVTVFPRASPSVQATYVLISGEDGRPKALMDG
                     AMLTARRTAAASALAAEMLAPPNAATLLMLGTGTLAPHLIEAHCSIRPIQRVLIWGRD
                     PRKAQALAGQLGAPGIEVSAVADRLSAISQADIISAATLSSEPLIRGDLVKQGTHIDL
                     VGAYTPEMCEADVACLGRARVFVDTRKGVLEEGGDVIQAIASGTLKVEDIEADLMQLC
                     SGQHGGRAGDATAITLFKSVGAAIEDLAAAELVFASWSTEAAPS"
     gene            7677..8927
                     /locus_tag="VFB37_16290"
     CDS             7677..8927
                     /locus_tag="VFB37_16290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO24057.1"
                     /translation="MSHKKIGVMCLLAALLYQSGAAAVPAAESSAVIIADRALDGLGG
                     TLRDVRIQIREGKIASLRGPMEARAIDLRGYTVLPGWIDAHVHIASHFDRTGHLATSK
                     EPAAEAALEMADAAWRTLLAGFTTVQSVGDPSEAPLRDAIRDHGFPGPRLLTSLAPIE
                     GKLSAATPDQLRAMVRERKAQGADLIKIFASSSQRVGAKPTLTQEQLDALCSEAKAVG
                     LRSMVHAYRGQVAAAARAGCREIEHATYATPEDLQVAVQAGAYLTPQVGLVVQNYIEN
                     QNRYIGIDGYTVEGMTVMLRDMPEDFAVCTRLVQMPGAKMVFGTDATAGAHGHNAEEF
                     IGRVQHCGQSRMAALVSANSVAAEAIGLGDEIGRLAPGYAADIIALDGDPLQDITAVR
                     RVVFVMRGGKIYKWVGSHAGHHTG"
     gene            9003..10598
                     /locus_tag="VFB37_16295"
     CDS             9003..10598
                     /locus_tag="VFB37_16295"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO24058.1"
                     /translation="MSRLLLPACVILLVSVSTAATPDKNTSAWWGHIKVLASDDFQGR
                     LTGSPGYHKAAEYVASNFERDGLKPAGSRGYFQSVSFEVQTIETGRTRVKLTRPGGAS
                     PLPVDESVVISPGVQQRPETRAPLIFAGNGIHLPEAGYDDFAGLDVHGAVVVYLVGGP
                     QSLSGAQRAHAVSEALPHFLEQAGAVGVVSLTAPKNREIPWARQKAAGTQPGMVLEEK
                     DLRRYQGPMFSAAFDESRADVLFEGSGHRFSELVALAESHKPLPHFRLATGIDAHVEA
                     KISTISADNVIGELPGSDPALAQQAVVLSAHLDHLGTGTPDHGDGIFHGAMDDASGVA
                     SLLEIAHRLQDSKARPRRSILFVAVCAEEKGLLGSRYFAAHPTRHAAQIVADINTDMF
                     LPLYPLKHVVAFGADESTLGDDVQAVASRMGVEVLPDPMPDHLIFVRSDQYNFVRKGT
                     PAVMLAMAPSPGTAEQATQQQWFSTRYHAQADDLSQPVDLAAADAYDKMVYTLAMRVA
                     DAPKPPSWHSDSFFARFAAHPLP"
     gene            complement(10642..11982)
                     /locus_tag="VFB37_16300"
     CDS             complement(10642..11982)
                     /locus_tag="VFB37_16300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO24059.1"
                     /translation="MIVSTAWLALVLAGPSARASDAGPHAFTNAVLIDGTGSPAVEHA
                     TVVIRGEKIETAGPSASVQIPRGASVTDLHGKVLMPGLADMHVHLVGGWDGHATDMLG
                     YRRYLNALLYAGVTTVLDTGNVQPYIVQMRAEVAAGRLPGPRIYCVGPLIDGADPVWP
                     PLSYSVPSVSQVPAVVTALKRDHVDLIKGYVGLSHPVLQRLVEEGRKVSLRVIVDQWE
                     RNGSHDLVEDGIYGFAHLPTRAMTASDVSFMKAHGTVFISTLVVYESFSRRRLRDLAF
                     LQEPLVRDTSPPSSLEGLKAEAARKLSSAEEEKTQRWAGYLRDAMANAQLLYHADVLL
                     AAGTDANYPGDMQGEGLHRELELLVEAGLTPLEAISIATRNAARLIGASDWGTIEPGK
                     LATLIVIDGRPDRDISQSRRIVSVFVRGKRIDRDSLRLARHNDPGFEPISPSDF"
     gene            complement(12037..14292)
                     /locus_tag="VFB37_16305"
     CDS             complement(12037..14292)
                     /locus_tag="VFB37_16305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO24060.1"
                     /translation="MKKIRSVLSSSVAMALAIATLAAAPLRAQTSAAPAAAATDSEGL
                     QEVVVSATKRTEDAQNVGLSLTALTAPQLESKGVEQFFDYGTAIPNLSFGIGAADGSL
                     AARGIALRGIQGANTTGFYIDDTPVLETLDPHIVDVARIEVLRGPQGTLYGAESMGGT
                     VRIITAQPDASAFSGQVHVGTSDTQHGSWNQLVEGDVNVPLVSDVLALRASAFYQFDD
                     GYFDKAIGPEAAPPTNVLHDIGSMKYYGGQIAMRFQPIEGLAFTPRIMYQRTDEGGVP
                     YALDYASNLLQRQMFNIDEGGTDKWWLASFTINYTVPFGSFVSSTAYFKRDTFETEDD
                     TDVLQYDLGLPAPIPSPITREIDLRRLAQELRFASTFPGPFQLILGGFYSGSTRPRDY
                     EWTGDGLGAATGTSSDLALSFIDSRHAGEYALFGDASYDILSNLKATVGVRWFRDTAT
                     FNQYTNGLFFGGAPAVYVAAPTSEKGFTPKYLLEYQATPQFLTYASAAKGFREGGNNI
                     ALPPGPPPGGCDADLANIGVTASQISTYKSDSLWNYEVGFKSTFLDRRFTFNAAGFVI
                     NWDKIQQLVSLPLCGYGFTGNSGAARSTGFELEFSGRPIPELTLGLGLGYDDAHITEQ
                     GLDSPQKIGSPVYQVPGITIASNAEYERHLNARWSGFGRVDFSHAGGSFSANNAQVNP
                     LYRPAYSLVDLRLGLRNDRYEVAAFVKNLANERANLGDAIMIGAQLPGQPHFVISRPR
                     TAGVEARMKFD"
     gene            complement(14454..15842)
                     /locus_tag="VFB37_16310"
     CDS             complement(14454..15842)
                     /locus_tag="VFB37_16310"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HZO24061.1"
                     /translation="MAAATLALAPLLLGAESARATPVQLAVTPEKGLVDSPFHVVVQN
                     VLPGSRVMVQASRPDAQGRRWTAVGEYSADAAGRVDLDSSPSLGGSYEGLSAHGLWCS
                     AMPVAPEQKHRYLQDLPEHPELGTAPHLAVGEVYAISLTAWIDGRQVASARATRTYGA
                     GITPVSVTTPEGVRGLFFPATPHQQPGVPVVVLAGSGGGLPDTQAALLASHGHPALAQ
                     GIFGYKDLPETMHNISLETFRAGARWLKQRTGAARVAIMGTSRGSEGAGLAASYFPED
                     FSAAILYVPSHLVNGAFGPGIRDVEPAWTYQGKPLAADHGETDSNNADDAMHSRQPPG
                     FIGTPYYLSTWSDAAVAARLGIPFERMSGPVLAIAGGADQMWPSFIGAAQIGHRLAAH
                     GLAQRAEIRIYPGAGHMVSRVGTGGPFSSFIFHPVAKDFEATGGLPNDNCEGSYAAWQ
                     EVLRFLARLQAR"
     gene            complement(15904..17013)
                     /locus_tag="VFB37_16315"
     CDS             complement(15904..17013)
                     /locus_tag="VFB37_16315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZO24062.1"
                     /translation="MVVGTVGTQDRARRRDLQESAPQDVLVARQPIVGMLQDDAERIL
                     GEMLGGSPASAVDVVPPARWAAASVRHYLHVGEGDGYWDFYRPWTALMLSVTDATYRT
                     DTWVRVEGTDYFKLRVLLSGTLRARSGQVIARAPEALLYVSPGATREGYYIGANEPIR
                     MVVLHCRPNLLNRVLGLDLSDVPSPLNSLFLPGRGAAHQRLTPSAEMIHVAGRIVQSR
                     HELSRALRDRHLQTLSIELLLQVLGILENRTLVQSGAGGISARDAARIYEARDYLAQH
                     YANPPNIPQLARMIGLNRTKLKESFRDTVGFTIYEYILQRRMERAAEMLVTGDYGVAQ
                     VAYAVGYEYPANFTAAFKRHFGQLPHTWKRRHLGH"
     gene            complement(16995..>17231)
                     /locus_tag="VFB37_16320"
     CDS             complement(16995..>17231)
                     /locus_tag="VFB37_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24063.1"
                     /translation="SRISLLSAASFEQRFRQPPSFGNRSEPLLAGFALGSDNVNRARE
                     FAERAGYRVQDTVEDGFFVHLPEEGVLIEWSSEL"
BASE COUNT         3139 a         5400 c         5591 g         3101 t
ORIGIN      
        1 agttctcgct gagcgagctg gcaacgcttg ccgatgtaag aggctggacg gctgtcctca
       61 tctggaacaa tcaaggctac cgcgagatcc gcacggccat gacccaggcg gggatcgagc
      121 ccgaaggggt ggatgtgcgt ccgccggatt tttcgcacat cgcccgtgcc tacggccatc
      181 cgcaccgccg gatcacctcg cgtgagtcgc tacgcaagtc gctccaggaa ttcgccagcc
      241 gtcgcggcgt cctgatgctg gaggtttccg ctgatgagtt cgcgtgaatg acagagaggg
      301 tgcaggcgat ctctatgcag acagcttcca gatacgatgt gatcatcgtc ggcggaggtc
      361 acaatggcct cgtgtgcggg gcgtacctcg ctcgcgcagg caggaaggtg ctcgttctcg
      421 agcgcaaccg gcaggtcggt ggcgcagcag tcaccctgga gttcgctcca gggtttcgcg
      481 cgtcgacctt ttcgtacctg atgagcctgc tgcatccgaa ggtcatgcgg gatctggagc
      541 tggaacggtt cgggctgcgg atattgccgg cgaccgacat gtttactccg ctcgcgggcg
      601 gaaattattt tcttttcacc ggcgacgacg agcgcaacgt cagggagatc gcccgattct
      661 ccgagcgtga tgcgcggcag tatccccgtt tcaagcgcta tctcgatcag attgcagccg
      721 ccgcgcgccg gctcatgctg cagacaccga tcgatccgca ggacctctct ctcaggggcc
      781 tggcggggct cgccagcttc gtgttcgaga accgcaagtc gctcaaggag ttctacgggc
      841 tctacgacat catgacgatg agcgcctacg atttcctctc cgaatggttc gaatccgacg
      901 tgttgaaagc cgtgtacgcc tactactcgg gaatcggcag cttccaggga cccaagaccc
      961 ccgggtctgc ctatatcctg ctgcaccacc tgatgggaga gcacgcgggg gccggcggct
     1021 ggggcttcgt ggaaggcggc atgggaaaga tttccgagac gatcgcgcaa tccgggctca
     1081 cgcacggaat gcacatcctc actgaggcgg acgtcgatca catcgagtcg agggaagggc
     1141 gcgcgaccgg tgtcgtgtgc cgtgacgggc gtgagttcca cgccaaggtt gtagccgcga
     1201 acgtcagtgc gaagctgctg ttcacacgta tgatgaatgg ggcgcagctt cccgaagagt
     1261 ttcgctggca cgtggagact tttcgtaccg agtccgccgc ggcaaagctc aacattgcct
     1321 gcgagcgcct gccgcagttc tccgcgttca acagcgagct gtgtggcttc gagtatcctt
     1381 cgtatgcgca tgtggcgccc gatgtcgcct acctggagca agcctatgcc gatgcgaagc
     1441 aagggtggta ctcgcagcgt cccttcgtga cggtgaccgc ccctacgacc gtggaccgca
     1501 cgctcgctcc tgcgggcaag cacgtcctca acctgttttg cggtcatgct ccctatacct
     1561 tgagaaatgg agcgagctgg gaggatcacc ggcagaaatt cgccgagctc gtgctcgatg
     1621 cgctcgagca gttcgctcca ggcttccgca aggacatcat cgccatgcag gtgctggtac
     1681 cgccggacat cgagcgcctc attggcagcc ccaacggtca tatctttcac ggcgagctct
     1741 cgatcgacca gctgttcttc aagcgcccgg cgccgcactt cgccaactac cgtaccccga
     1801 tccgcgccct gtacctgtgc ggctcgacga cccatcccgg aggcggggtc tcgggcatct
     1861 ccgggcacaa tgctgcgcgg gagatccaga aagactggcg tcgcctgtag ccgagcgatg
     1921 tccgtgcggt taaaaaagcg gaacctctcg aaggcggtga tcgcctcgcg tgagtggtac
     1981 gccagactgg gggagctttg cgccggtggc ttcacgacgg cgagcatgat cggtcttgca
     2041 gatgagctcg tggagcggct cgatgtggac agcatcctga tcctgcgcta tcccagggcc
     2101 tcatcgccgc agcttctgta cggccgtccg gatcaccggc accgtgccaa tacagtggag
     2161 gattacctgc tcggccacta cgcgctcgat cccttctacc tgcgcagcga ctactgttca
     2221 cagcggggac tcatttccct gcgggatgtc atcgaggagg actttgcctc cagcgagtat
     2281 tacaaggtgc actatcagtc ggcaggattg atcgacgaga tgtgcttctg ctgcagcgat
     2341 ggcgagggcg gtcatctcaa tctttcgctc agccgcaccc tcggcaagag ccatttcagc
     2401 gccgcggagc tcgcaggggc tcgagcgatc gcaccgctgg tgacggcggc cctgagctca
     2461 acgtggcgtg ctctctgccc ggatgccagc agcgctcctc aggtgacctc cgcagacggc
     2521 gagcatcacc gtcacatcga gaacgtccgg ttgaatttcg gacgctcggt gctgacggag
     2581 cgggaatacg agattttgca gcacctgctg cggggaaatt ccgtcgatct gatcgcccaa
     2641 cgcctggata tcgccgtcag caccgtgaaa gtgcatcgca agcacatcta ctcgaagctc
     2701 gagatcaatt cgcaggccga gatctttacg ctgtttctgg aggtggtggc tggaacgaag
     2761 tacgagcccg gctccgaccc gctggcgcgc taccagagat cgagcgctcg agcgtgagtg
     2821 gaatgcctca ggcagattgc tgtgcggcca gcaccagcag cgagcaggcc agctgggtca
     2881 ccttgctcag aggcgccgtc gggtggcgcg ccgttccgta caggacgttg gagccttcgc
     2941 tgatgatgcc catgagctgg acgatcgcgc ggcaggaggc gggcggcaga ttcgggaagt
     3001 tatgtcgcag gtgctgcgag gccgtctcct ggacctcgcc gtagaagcgg tctacggcct
     3061 tcgcgatgaa ggggtcccgc agggcgatgg tccagaattc cctgaacagc cggctggtct
     3121 gcgcggaggc ggagtcgcgc atcagccagg cgacgaggga gcggaaggcg accggcgagc
     3181 gactcggagc gacgcgcatg cgcgcaacga tctgcgcccg atactcctcg atcaacagca
     3241 tgatcaaggc gcgcacgaga ttgcgcttcg aggggtaatg gtaggcgaga ttgccgacgg
     3301 tcagccctgc ttcattggcc actcgtctga gcgtgaagtg cgcatatcca tgggttgcca
     3361 ggacccgctt ggccgcgcgc agaatttcta tgcgagtgcg ctcacccgag gcacggctgc
     3421 cactgcgggg cgaacgcggg gtgctcgtcc cttccagaag cgctgcgctt tccccgacca
     3481 tcatcgaacg cgtggcgcac attggatcct cctgtgcact cactatagcg ccggggggca
     3541 ggcgatacca aggcgcgcgg aggcaattga gcttcgcaaa cccgcaagct attgctggtt
     3601 aggttgtgca agcgtgcgcc agtagcgctc gcgcacgatc ctgacgtgca accactgctc
     3661 gcagtactcc accgagggca aagacctgag tcgtgagacg agcagattga agtcccgcag
     3721 cgagtcgaac tccacggtgg cgagcagatc gaatcgcccc accgtgcgcg cgatgaattc
     3781 aaggccgcgg ttggccgcga tcgcctcgat ggcctcgcga cagtcgtcgt gggcattcac
     3841 gccgatgccg aagacgagat cgctggtcat gtcggcgcgt tgcttgatgg cgcctatctg
     3901 gatgacgccg gattcgagca gccgctgcac gcgggtccgg caaccgctca ccgagaggcc
     3961 gacgacctgc gcgatctcgg cgaagctggc cctgccgtcg tgcagcagcc ggttgacgat
     4021 gttgatgtcg acctcgtcga actgcgacag tgactccggt cctgtccccg cgtagaagct
     4081 gctcaggatg cgctcgtaga gatgcacaaa gacttcacgc acacccttga tggctcgaat
     4141 ggcgagcacc tcgcgattga gctcggtgag gctgcggagc tgcaactcga ccacgatctg
     4201 aaaagcgccc gcgacgacgg agatcagcac gggcgcatcg aacttctcca gcgcttcgat
     4261 cacgctctgg atgttgccgg cggccttgat cgacagatgt gccgaggtcg tgaggccgag
     4321 cactcgtgga tgcacactcg ccacgatgct gagcttcccc gactcgatca gtggattgat
     4381 ccggctcgcc acgtagtcgc gattcgtccc gagctcgcgc gcgatctcgg aatacgaggc
     4441 acggccgttg cgccgcagga ttgcgatcat ctgtgcagtg agatcattca tgctgcggga
     4501 gggtgaatga tcgagcggac acgcacatta ctgctttcga tctggagggc gcgatcagtg
     4561 ccgctctgct cttctaggag attgcttcgc gctcgggtac tctagcagcg gaccaagtgt
     4621 agggcaaccg ctctaatctg tccgcagcga gcgcgaagcc aggattgcga tgagagcagc
     4681 acaccacccc ggcgcgctac atgtcagctc ccacggcctg tggcagcgga ccgcgccggg
     4741 catggccgag ctgcgcgggc tgagcgaaga tcttaccgcc gatgtcgctg tgatcggcgc
     4801 aggttacacc gggctctcca cggcattgca cctcgttgag aatcgatctt cggtcgtcgt
     4861 gctggaggct gccgatattg gattcggtgg ctcggggcgc aatgtcgggc tggtcaacgc
     4921 cggcatgtgg gtcatgcctg atacggtcgt acagcgcctg ggcagcgagt ttggaacgcg
     4981 gctgctgcag ctgctgtccg ccgcgccgga cgtcgtattc aacatcatca gcaggtatgc
     5041 gatcgactgc gaggcgaggc acgcggggac gctgcattgc ggagtaggtc gcaaggggct
     5101 tgccgaattg cgccagcgcg ccgcccagtg gcaggcgcgg tccgcgcccg tagagctgct
     5161 ggaggcggcc gagacgcgcc gacgcgttgg atcggcagcc ttcagtggag cgctgttcga
     5221 cccgcgcgcc ggaaccatcc aaccactggc ttacgcgcgt ggacttgcgt ccgctgcact
     5281 cgcccaggga gccaggatat tcgtgcgcag tccggcgacc acggttgaac cgtccgggtc
     5341 cggatgggtg gtgcgcagcc cttcaggcag cgtgcgtgcc gaatggatcg tcgtggcaac
     5401 cgacgcttat gcaagagggc catgggatat cgtgcgccgc gagcaggtgc acctgcctta
     5461 cttcaacttc tcgctgccgc cgctcgagcg ctcactgcgt gattcgatac tgccggcagg
     5521 cgaaggggcc tgggacacgc ggtccgtgct ctcctcgttt cgactcgatg cagcgggccg
     5581 gctcatcttt ggcagtgtcg gagcgcttcg aggcgcagac gggggagtcc acaggcaatg
     5641 ggcgcttcgg gcgatgcgca gaatatttcc gcaactcgag ggattgcagt tcgagacggg
     5701 gtggttcggc cacatcggca tgacctcgga caatctgccg cgctttcacg tcctggcccc
     5761 gcgggtgatc ggcttcaacg gctacaacgg gcgaggcatc ggtcccggaa cgaccttcgg
     5821 aagagtgctt gctgcattcg cgctgggaca gctgcgcgag caggatctgc cgctgcccgt
     5881 gtccacgccc aagcgcgttg catttcgatc ggttcgggag gcgatctacg cgcagggatc
     5941 gcaaatgcta cactttgcag gagcaagatt ttgagccagt cgtctgcgca aaccgggttc
     6001 accggcaagg caccggctcg gccattcgtt cgtgtttacc caggagaaga cgatgaaagt
     6061 agtttctgcc cactcgagga tccaccgggc tctgggcgtc ctcacggcta cggcgctgct
     6121 tctggttacg gcttccgcag cggcggagga tgtcaaggtc agcctgaccg gctcagagga
     6181 aacaccgccg gtgaccacct cagcgaccgg tagcggaacg attcacatcc acaaggacaa
     6241 gtccgtcacc ggtacgatca agaccaaggg aatcgaggga acggctgcac acattcacct
     6301 cggcgccccc ggtcagagcg gcccgccgat catcactctc acgaaggcag ccaatggggc
     6361 ctggaccgtg ccgaagggct ccaagctgac cgacgagcag taccagagct tcaaggatgg
     6421 caatctgtac gtgaacgtcc acagcgcgga gcacaagccg ggtgagattc gagcacaact
     6481 gaagccctga ggatgcgccc ggttggcccg ctgtccgacc gcgagccagc cggcgctcaa
     6541 ctgtcgaagt ggagccgaca ggtggctacg accaaccgcc gcctggcttc gatgcgccag
     6601 gcagcccctc agctgggggc tgcctctgtg ctccacgagg cgaacacaag ttccgcggcc
     6661 gcgagatcct cgatcgcggc accgactgac ttgaagaggg tgatcgccgt ggcatctccc
     6721 gcacggccac catgttggcc tgagcacagc tgcatcaggt cggcctcgat gtcctcgact
     6781 ttcagggtac ccgaggcgat cgcctgaatc acatcgccgc cttcctccag caccccttta
     6841 cgggtatcga cgaaaacacg agcccgcccc aggcaggcga catcggcctc gcacatctcc
     6901 ggcgtgtagg cgcccaccag atcgatgtgg gtgccctgtt tgacgagatc tccccgtatg
     6961 agcggctcgg aggacagtgt ggcggccgag atgatgtcgg cctgcgagat tgcagaaagg
     7021 cgatccgcga cggcgctgac ctcgatgccc ggagcgccaa gctgccctgc gagcgcctgc
     7081 gccttacgtg gatcccggcc ccagatcaaa acccgctgga tcggtcgtat gctgcaatga
     7141 gcctcgatga ggtgcggcgc cagggttccg gtaccgagca tcaacagagt ggccgcgttc
     7201 ggcggtgcca gcatctccgc cgcaagcgcc gacgcagcag ctgtccgtcg cgccgtcagc
     7261 atcgccccat ccatcagtgc cttcggcctt ccatcctcgc cgctgatcaa tacgtacgtg
     7321 gcttgcaccg acggactggc gcgaggaaac actgtcacga tcttcacgcc cgcatcgccg
     7381 ccatcgcgcc atgccggcat cagcagcagc gtggagttgc ctggaagctg gaaccgctgg
     7441 cgcgtgggca tagcctcagc acgccggaag gcctcgcgca gtgccgggac caacccgcgc
     7501 atcggcgtca gcctccggat cacggcagcc gggatgaagg aggcattcgt tcggtccggg
     7561 ccgctgctca ccgcagggat tacatctaca aagccgattt ctgtcaatga ggcacccggc
     7621 ccccttgctc gggccctgct aagataggtc tatcgaccta gacgcgggag cgtcggatga
     7681 gccacaagaa gattggcgtc atgtgtctgc ttgcggcgct gttataccag tccggtgcag
     7741 ccgcggtgcc tgcggccgag agctcagccg tcatcatcgc cgatcgcgct ctcgatggcc
     7801 tcggcgggac attgcgtgat gttcgaatcc agatcaggga aggcaagatc gcctcactcc
     7861 gggggcccat ggaggcgcgc gccatcgatc tccgcggcta caccgtgctg cccggctgga
     7921 tcgatgcgca cgtgcacatt gcctctcatt tcgatcgcac cgggcatctg gcgacttcga
     7981 aggagcctgc ggccgaagcc gcgctggaga tggcggatgc cgcgtggaga acgctgctgg
     8041 cgggcttcac caccgtgcag agcgtcggcg atccgagcga ggccccactg cgggatgcga
     8101 tccgcgacca cggttttccg gggccccgtc tgctgacctc gctggcaccg attgaaggca
     8161 aactgagcgc ggctaccccc gatcagctgc gggctatggt gcgcgaacgc aaggcgcagg
     8221 gggcggatct catcaagatc ttcgcctcgt cgagccagcg cgtcggggcg aaaccgacgc
     8281 tcacgcagga acagctcgat gctctgtgca gcgaggcgaa ggctgtcggg cttcgttcga
     8341 tggtgcatgc gtatcgtggg caggtcgccg cggcggcgcg ggccggctgt cgcgagatcg
     8401 agcatgccac ctacgccacg cccgaggatc tgcaggtcgc cgtccaggcc ggggcctatc
     8461 tcactcccca ggttggcctg gtggtccaaa actacatcga gaaccagaac cgctacatcg
     8521 gaatcgacgg ctacaccgtg gagggcatga cggttatgtt gcgggacatg ccggaggatt
     8581 tcgccgtatg cacgcgcctc gttcagatgc ccggtgcaaa gatggtgttt ggtacggatg
     8641 ccaccgcggg tgctcacggc cacaacgccg aggaattcat cggccgcgtg cagcattgcg
     8701 gccagtcccg catggcggcg ctggtctcgg cgaactcggt cgccgcggag gccatcggcc
     8761 tgggcgatga gatcggccga cttgcgccgg gctatgcggc ggacatcatt gccctggatg
     8821 gcgatcctct gcaggacatc actgcggtcc gtcgtgtcgt attcgtgatg cgcggcggga
     8881 agatctacaa gtgggtggga tcacacgccg gacaccacac cggctaacgc atggccgcaa
     8941 ccgaaccatc gtggtacaac gcgagccgcg ggatccataa caacctgaaa tggagcagcc
     9001 ggatgtcgag acttctcctg cctgcctgcg taatcctgct cgtgtcggtg agcacggccg
     9061 caacacccga caagaacacc agtgcctggt gggggcacat caaagtcctt gcctcggacg
     9121 acttccaggg acgcctcacc ggcagtcccg gctaccacaa ggcggccgag tatgtggcca
     9181 gcaacttcga gcgcgatgga ttgaagccgg cagggagccg tggctatttc cagagtgtca
     9241 gcttcgaagt ccagacgatc gaaactggcc gtacgcgggt aaagctgacg cgtccgggcg
     9301 gcgcatcacc tctgcccgtc gatgagagtg tggtcatctc gccaggggtt cagcagcgac
     9361 cggagactcg cgcgcccctg atatttgcgg ggaatggcat tcacctgccg gaagcaggct
     9421 acgatgactt cgcaggcctc gacgttcacg gtgccgttgt cgtgtatctg gtaggcggtc
     9481 cgcagtcgct ctccggcgcc cagcgtgcac atgcggtatc ggaagctctt ccgcacttcc
     9541 tcgagcaggc cggtgcggtg ggtgtggtct cgctgaccgc gccgaagaat cgtgagatcc
     9601 cctgggcgcg gcaaaaggcg gccggcactc agccgggaat ggttctggag gaaaaggacc
     9661 tgcgccgcta ccaggggccg atgttctcgg ctgccttcga tgagagtcgg gccgatgtgc
     9721 tcttcgaggg ctctggccac aggttttccg agctcgtcgc actcgctgaa tcacacaagc
     9781 cactgcctca cttccgtctc gctacgggaa tcgatgcgca cgtcgaagcg aaaatctcca
     9841 caatctcagc cgacaacgtg atcggggagc tgcccggcag cgatcctgct ttggcgcagc
     9901 aagccgtcgt gctctctgca catctcgatc atctcgggac ggggacgccc gaccatggtg
     9961 acggaatctt ccacggcgcc atggatgatg cttcgggcgt ggcctcgctc ctggagattg
    10021 cgcacaggct acaggattcg aaggcacgcc cgcgacgctc gatcctcttc gtggcggtgt
    10081 gcgccgagga aaagggactg ctcggctcgc gatacttcgc ggcacatccg acgcgccacg
    10141 ctgctcagat cgtggccgat atcaacacgg acatgtttct gccactgtat ccgctgaagc
    10201 acgtggtggc attcggtgcc gacgagagca cgctcggaga tgatgtgcag gcagtggcca
    10261 gccggatggg ggtcgaggtc ctgcccgatc cgatgccgga tcatctgatc ttcgtccgct
    10321 cggatcagta caacttcgtg cgcaagggga cgcccgcggt catgctggcg atggctccaa
    10381 gtcccggcac cgcggagcaa gccacccagc agcagtggtt cagcacgcgc tatcacgccc
    10441 aggcggatga tctgagccag ccggtcgatc tcgccgcggc cgacgcttac gacaagatgg
    10501 tctacacgct cgcgatgcgc gtggcggatg cgcccaaacc accatcatgg cattcggaca
    10561 gcttcttcgc gcgcttcgcc gctcatccgc tgccatgaat gcacttgcgt gcgagctgac
    10621 agctcgcgca gcggggtccg ctcagaagtc gctgggtgag atgggctcga agcctggatc
    10681 gttgtggcgt gcgagccgca ggctgtcgcg atctatccgc ttgccgcgca cgaagacgct
    10741 cacgatccgc cgtgattgag agatgtcgcg gtccgggcga ccgtcaatga cgatcaatgt
    10801 ggcgagcttg ccgggttcga tcgtgcccca atcgctcgca ccgatgaggc gcgccgcatt
    10861 gcgcgtggcg atcgagatag cctccagcgg cgtcaggcca gcctcgacca ggagctcgag
    10921 ctcgcgatgc aggccctcac cctgcatgtc gcccggatag ttcgcgtcgg tcccggccgc
    10981 cagcagcacg tctgcgtggt agagaagttg cgcattggcc atcgcgtcgc gaaggtatcc
    11041 ggcccagcgc tgtgtctttt cctcttcggc agagctcagc ttgcgggcag cttcggcttt
    11101 aaggccctcc agagacgagg gcggactcgt gtcacgtacc agcggctcct ggaggaaggc
    11161 gagatcgcgc aggcgccgcc ggctgaagct ctcatacacc accagagtgg agatgaacac
    11221 ggttccatga gccttcatga agctcacatc ggatgccgtc atggcgcgag tcggcagatg
    11281 tgcaaatccg taaatcccgt cctcgaccag atcgtgcgag ccgttgcgct cccattgatc
    11341 gacgatcacg cgcaacgaca ctttgcgacc ctcctcgacg aggcgctgca gcaccggatg
    11401 cgacaagccc acgtagccct tgatgaggtc cacgtgatcg cgcttgagcg cagtcactac
    11461 cgcaggcact tgtgagaccg agggtacgga ataggaaagg ggcggccaga ctgggtccgc
    11521 gccatcaatg agcgggccga cgcaatagat gcgtgggccg ggcaggcggc cggcggcgac
    11581 ctcggctcgc atctgcacga tgtagggctg aacgttaccg gtatccagaa ccgtggtcac
    11641 gcccgcatac aacaatgcat tcaggtaacg gcgatagcca agcatgtcgg tggcatggcc
    11701 atcccagcca ccgaccaggt gcacgtgcat atcggcgaga ccgggcatca gtaccttgcc
    11761 gtgcagatcg gtaacgctgg cgccacgagg gatttgcacc gaagcggacg gacctgcagt
    11821 ctctatcttc tcgccgcgga tgacgacagt ggcgtgctcg accgccggac tccctgtgcc
    11881 gtcgatcagt acggcgttgg tgaaggcgtg ggggccggca tcgctggcac gggcgctggg
    11941 cccggcgagc acgagagcca accatgcagt ggatacgatc acggccgcgc gtgcggatcg
    12001 gctcggtatt tggacggctg gcatcgcgac cctgcgtcag tcgaacttca ttcgggcttc
    12061 gacgcccgcg gtgcgcggcc ggctgatcac gaagtgcggc tggccgggca gctgtgcgcc
    12121 gatcatgatg gcatctccca ggttcgcgcg ctcgttggcc aggttcttga cgaatgccgc
    12181 cacctcgtag cgatcgttgc gcaagcccag gcgcaggtca acgaggctgt atgccggacg
    12241 gtatagcgga ttgacctgcg cgttgttggc ggagaagctt ccccctgcgt ggctgaaatc
    12301 gacgcgcccg aatccggacc agcgggcgtt caggtggcgc tcatactcgg cattgcttgc
    12361 gatcgtgatg ccgggaacct ggtagacggg gctgccgatt ttctgcggac tgtcgagccc
    12421 ctgttcggtg atatgcgcgt cgtcatatcc cagccccaat ccgagcgtga gctccgggat
    12481 gggtcgcccg ctgaattcca gctcgaaccc cgtgctgcgt gccgcgcccg agttgcccgt
    12541 gaacccgtag ccgcacagcg gcaaggacac cagctgctgg atcttgtccc agttgatcac
    12601 gaacccggcc gcgttgaacg tgaagcggcg atcgagaaag gtcgacttga accccacttc
    12661 gtagttccac aggctgtcgg atttgtaggt actgatctgc gaggcggtga cgccgatgtt
    12721 ggcgagatcc gcatcacagc cacccggagg aggcccgggc ggcagagcga tgttgttgcc
    12781 tccctcgcgg aagcctttgg cggcacttgc gtacgtcagg aactgcggcg tcgcctgata
    12841 ttccagcaga tacttcggtg tgaatccctt ctcgctcgtg ggcgccgcca cgtacactgc
    12901 aggtgcaccg ccgaagaaaa gtccgttggt gtactgattg aaggtggccg tatcccggaa
    12961 ccagcgcacg ccgactgtcg ccttcagatt gctgaggata tcgtaggatg catccccgaa
    13021 cagcgcgtac tcaccggcgt ggcgcgagtc gatgaacgaa agggcgagat cgctgctcgt
    13081 gccagtggcc gccccgagtc cgtcgcccgt ccattcatag tcacgcggac gagtgctgcc
    13141 tgaatagaag cctcccagta tgagctggaa gggtccgggg aaagtcgagg cgaagcgcag
    13201 ctcctgagcc aagcgtcgca ggtcgatctc acgcgtgatc gggcttggga tcggcgcggg
    13261 gagcccaaga tcgtattgca ggacgtcggt atcgtcctcc gtctcgaagg tgtcgcgctt
    13321 gaagtaagcg gtcgaggaga cgaacgagcc gaagggcacc gtgtagttga tcgtgaagct
    13381 ggccagccac cacttgtccg ttccgccttc gtcgatgttg aacatctggc gctgcagcag
    13441 gttgctcgca tagtcgagcg cataaggtac gccgccctca tcagtgcgct ggtacatgat
    13501 tcgcggcgtg aaggcgagtc cctcgatcgg ctggaaccgc atggcgatct gcccgccgta
    13561 atacttcatg ctgccgatgt catgcaggac attggtgggc ggtgcagcct cggggccgat
    13621 ggccttgtcg aagtaaccat cgtcgaactg atagaacgca ctcgcccgca gcgccagcac
    13681 gtcgctgacg agtggaacgt tgacgtcacc ctcgacgagc tggttccagg acccatgctg
    13741 agtatcggac gtgcccacgt gtacctggcc gctgaaggcg ctcgcatccg gctgtgcggt
    13801 gatgatccgg acagtgccgc ccatcgattc cgcgccgtac agcgtgccct gcggaccccg
    13861 cagcacttcg atgcgagcca catccacgat atgcggatcg agcgtctcca gcacgggcgt
    13921 gtcatcgatg tagaacccgg tcgtgttggc cccttgaatg ccacgcaggg caatcccgcg
    13981 tgccgccagg gagccgtctg cggcgcctat tccgaaggac agattgggga tcgccgtgcc
    14041 gtagtcgaag aactgctcga ctcccttgga ctcgagctgc ggcgctgtca aagccgtgag
    14101 gctgaggccg acgttctgcg catcctcggt gcgtttggtg gcggagacga ccacctcctg
    14161 caacccctcg gagtccgttg cggctgcggc cggcgcggca ctggtctggg cgcggagcgg
    14221 ggcagcggca agtgtggcga tggccagagc catcgccacc gagctcgaca gcacagatcg
    14281 aatcttcttc accgaactcc cccgttacat gactgcgccc gcccgcgggc attcgactgc
    14341 caagatgagt cgatgttagg gagttcggct cgtatcaaag catgcgattc gacgggaaaa
    14401 gcatggggcc ggacgctgcc ggtgctcttg cggaggaatg tatccgcccg gtcctagcgc
    14461 gcctggagcc tggcgaggaa gcgcaggact tcctgccagg ctgcgtaaga gccttcacag
    14521 ttgtcgttcg gcagcccgcc cgtggcttcg aagtccttcg ccacgggatg aaagatgaaa
    14581 gacgagaacg ggccgcccgt gccaacccgg gagaccatgt gccctgcacc gggataaatg
    14641 cggatttcgg ctctttgcgc cagaccgtgc gctgccaggc ggtgcccgat ctgcgctgcg
    14701 ccgatgaagg acggccacat ctggtcggcg ccccctgcga tggccaacac cggtcccgac
    14761 atcctctcga acgggatgcc gaggcgggcc gcgaccgcgg catcgctcca ggtcgagagg
    14821 tagtagggcg tgccgatgaa gccgggtggc tggcgcgagt gcatcgcatc gtccgcgttg
    14881 ttggaatccg tctcgccgtg atccgccgcc agcggcttgc cctggtacgt ccaggcgggc
    14941 tcaacgtccc gaataccggg accgaacgcg ccgttcacga ggtgagatgg aacgtaaaga
    15001 atggcggcgg agaaatcctc ggggaagtaa cttgcggcga gccctgcacc ctccgagccg
    15061 cgcgaggtgc ccatgatcgc cacacgcgcg gcccccgtcc gctgcttcag ccagcgcgca
    15121 ccggcgcgaa acgtctccag cgaaatgttg tgcatcgtct cgggcagatc cttatagccg
    15181 aagatgcctt gcgccaaggc aggatgaccg tggctcgcca gcaacgccgc ctgtgtgtcc
    15241 ggtaatccac cccctgagcc ggccaacacg acgacgggca cacctggctg ctgatgcggc
    15301 gtggccggaa agaaaaggcc gcgtacccct tcaggggtag tgacgctgac aggggtgatg
    15361 cccgctccgt aagtccgcgt ggctctggca gaggccacct gccggccatc gatccaagca
    15421 gtcagcgaga tcgcgtacac ctcaccgaca gccagatggg gcgccgtgcc gagctcggga
    15481 tgttccggca gatcctgcag gtatctgtgc ttctgctccg gcgccaccgg catcgcagag
    15541 caccacaggc cgtgggcgct gaggccctcg tagctgccgc cgagggaggg ggaactgtcg
    15601 aggtcgaccc gtccagctgc atcggcggag tactcgccca cggcggtcca gcgtcgacct
    15661 tgtgcatcgg gacgcgaggc ttgcaccatg actcgcgaac ccggaaggac attctgcacc
    15721 acgacgtgaa agggggaatc caccagcccc ttctctggtg ttacggcaag ttgcaccggc
    15781 gtggcccgag ccgattcagc accaagcagc agtggcgcga gcgccagggt cgcggcagcc
    15841 actttgcaca cgcagggctc gcgccgcagc aggcgcagga cactgagcgg cgcgcaaagc
    15901 atatcaatgc ccgaggtgtc ggcgcttcca ggtgtggggc aactgaccaa agtggcgctt
    15961 gaatgcggcg gtaaagttgg cgggatactc gtaaccaacg gcataggcca cttgtgcaac
    16021 accatagtcc cccgtgacca gcatctcggc ggcgcgctcc atccgccgct gcaggatgta
    16081 ttcgtagatg gtgaatccta ccgtgtcgcg aaagctttcc ttcagcttgg tgcgattgag
    16141 cccgatcatc cgcgccagct gcggaatgtt gggcggattt gcgtagtgct gtgcaagata
    16201 gtcgcgcgcc tcgtaaatgc gtgcggcatc gcgggcgctg atgccgcctg ctccactttg
    16261 caccagcgtc cggttttcga ggatcccgag cacttgcagc agcagctcga tgctcagtgt
    16321 ttgcagatgc cggtcgcgca gggcgcgtga gagctcgtgg cgcgactgca cgatgcgccc
    16381 ggccacatgg atcatttccg cgctcggtgt cagccgctgg tgggcagcgc ctcttccggg
    16441 aagaaacagc gagttgagcg gagaggggac gtcgctcagg tccaggccca gcacgcgatt
    16501 cagcaggttg ggccggcaat gcagcacgac catgcggatc ggctcgttgg cgccgatgta
    16561 gtagccctcg cgagtagccc cgggcgagac gtacagcagg gcctccggtg ctctggcaat
    16621 gacctgcccc gatcgcgcac gcagcgtccc ggagagcaga acccgcagct tgaagtagtc
    16681 cgtaccttcg acgcgtaccc aggtgtcggt gcgatacgtg gcgtcagtta cagacagcat
    16741 cagcgccgtc catggcctat agaagtccca gtaaccgtca ccttctccga cgtgcagata
    16801 atggcgaacg ctcgctgcag cccagcgcgc cggcggtacg acatctaccg cggaggccgg
    16861 tgatccgccc agcatttcac ccaggatccg ctcggcatca tcctggagca tgccgacgat
    16921 gggttgtcgg gccaccagaa catcctgggg agcactctcc tgcaggtccc ttcggcgggc
    16981 ccggtcctgc gtgcctacag ttccgacgac cattcgatca gcactccttc ctcggggaga
    17041 tgtacgaaaa atccatcttc gaccgtatcc tggacacgat agccggcgcg ctcagcgaac
    17101 tcgcgtgcgc gattgacatt gtccgaaccc agcgcgaagc cggcgagcaa tggctcgctg
    17161 cggtttccga agctgggggg ttggcggaag cgctgctcga aggaagcagc cgagagcagg
    17221 ctgatgcggg a
//
LOCUS       DASRHN010000402        10593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_638368, whole genome shotgun sequence.
ACCESSION   DASRHN010000402 DASRHN010000000
VERSION     DASRHN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638368"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VFB37_16325"
     CDS             <1..141
                     /locus_tag="VFB37_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZO24064.1"
                     /translation="KAAPGTIRADHALSIEQNVVHGSDAADTAATEIAYFFRATELHP
                     RG"
     gene            157..1323
                     /gene="rlmN"
                     /locus_tag="VFB37_16330"
     CDS             157..1323
                     /gene="rlmN"
                     /locus_tag="VFB37_16330"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZO24065.1"
                     /translation="MNASPSPLAAKPTNLLGLPRPQLESLVAELDGKPYRARQLMSWI
                     YKRGVASMHEMTDLAKSLRTVLAERAEVRTPQIVTKHVSTDDTCKWVLRADESQAFEM
                     VFIPEPDRGTLCISSQVGCALDCSFCSTAQQGFNRNLTTAEIVGQVWLAKQQLGEYQA
                     AETAAQGARAAVGGDARRVTNVVLMGMGEPLANFRNVVAALHILLDDYGFDLSRRRVT
                     LSTSGLVPQIYRLAEETNVALAVSLHAPDDELRSQLVPINRIHPIAELLEACWHYIDE
                     QNGRSVTFEYVLLDGVNDSPAQARALAQRLRGHPAKVNLIPFNPFPGTHYRRSAMPAI
                     QRFRDELIQRGVLATVRRTRGDDIDAACGQLAGRVIDRTTVRLGSKVFGVAVQS"
     gene            1320..2102
                     /gene="pilW"
                     /locus_tag="VFB37_16335"
     CDS             1320..2102
                     /gene="pilW"
                     /locus_tag="VFB37_16335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HZO24066.1"
                     /translation="MTTVAARMSGRCRAAALCGALLALGLGGCSSQHAKQKHDAANYN
                     MQLGMAYLNRGELALAKDKLERALKENPEDPNVHSAMAMLQDRLGNADKADAEFKAAL
                     RLGPREPDILNNYAVYLCRTGRTDEGVKSFEEAAHNPLYRTPEVAYSNAGVCLRAAKR
                     DTQAAMSFQQALQIKPNFAEAAYQLGELDFERGELNESRDEVDRYLGAFEATPDLLLL
                     RVRIARKQGDRLSAERYSSQLRRDFPGSAQARVLAGLGRDSG"
     gene            2107..3063
                     /locus_tag="VFB37_16340"
     CDS             2107..3063
                     /locus_tag="VFB37_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZO24067.1"
                     /translation="MAGDAAGIGARLRAGREKLNLTVLQAAERMHVDPRLLESLEAED
                     FAALGAPVYTRGHLRNYAELVGESAPQLSELLSRVELEGQPDLTRIAKAPPSVESSKL
                     ILPALFVLAVFAVAGAVWWVLSLAPQQPIPTNELSAPPNADRASPSGSGGASPAIRPG
                     AVPPHAAVGPTHLASAGGPITVPSGTRGAVSVTQIPDRGSSATAPPSTALPPQPDSAP
                     APHPSREQQVTLHYSADCWTEVYDSSGQRLYYDVGAAGSTRTLKGTAPLRVVLANAAG
                     VAVEVDGRGTSIARMAQPDGSAEFLVNRSGRVARTRAPSSGD"
     gene            3066..4352
                     /gene="hisS"
                     /locus_tag="VFB37_16345"
     CDS             3066..4352
                     /gene="hisS"
                     /locus_tag="VFB37_16345"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZO24068.1"
                     /translation="MPDQIQPVRGMNDIPPAEIAAWQFLERCIAELVTAYGYEEMRVP
                     LIEHTALFKRAIGELTDVVGKEMYTFESQGGESLTLRPEATAGMVRALISNRMLRGQR
                     HKLWCIGPMFRHETPQAGRYRQFHQLDVEAVGFTGPDVDAELIALSARLWRLLGIEHV
                     RLQVNSLGTPESRRAYRELLVGYFRDHEAQLDADSRRRLGGNPLRILDSKSPQMQAIV
                     AGAPLLTEHLDPQSREHFEAFCALLRDFGVEYEINPRLVRGLDYYTRTVFEWITESSG
                     AQNAVCSGGRYDGLIAQLGGEPTPAIGFALGMERVVGLLVQAGRAPPPAKPDVFVILS
                     GDRAGTAGLGLIERLRNELPHRRFECNLGGGNLKTQFRRADRSAAPLALIVADDELDR
                     GVVAMKPLRQDSGQSECPIAQLAAGIEQALAALGVS"
     gene            4368..5057
                     /locus_tag="VFB37_16350"
     CDS             4368..5057
                     /locus_tag="VFB37_16350"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO24069.1"
                     /translation="MEEYLSEKEQWEAIKVWLKENLLWIVAGIAVGAAGLSGWRWYQA
                     HVNEVAIQASSRYEQLFDAVGKGDRARALVLLGELERDYSSSPYLDQARLLVARAYVE
                     THDLDKAAGELQAVAAHTKDSEVAMIARLRLARVQIAQQKPDAALTTLNGMEPGAFAW
                     RYHEVRGDAYYAKGDKANALKEYLSAKTADMGGGPDGADLDLKISDLSDQTPHLAGPS
                     LATPATAATSK"
     gene            5080..6588
                     /gene="bamB"
                     /locus_tag="VFB37_16355"
     CDS             5080..6588
                     /gene="bamB"
                     /locus_tag="VFB37_16355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HZO24070.1"
                     /translation="MRSRSWRGTVAALGMLGLLAACSKDKDIDQPAPLVTFNSTLKVE
                     RAWSNAVPDKGGKVLRLGLGLAVEGNRVFAAGHRGEIAAFDLHSGHVLWRTKSKEAFS
                     GGPGAGAGLVIVGASTGDILAVDESNGATRWKARLNGEVLSAPAVSEKLVAVRTVDGK
                     LHGLSPKDGHELWSQEEQVPRLSLRGTSHPVMTGELVLCGFDNGKVAAVNMNDGSVQW
                     STTIAPPHGKTELERLDDVDAAVRVAGADVYAVGFQGRIAMLALDTGQIWWSHDASSY
                     RGLGLDDSALYVSTADGEIVALRRRTGTEIWRQKALLHRRLSAAVETENAIVTGDFQG
                     YVHWLNKATGAIEARARTGKVRISNPPLAVGNMVLVVNDDGHITAFRTTPLGGGSRGK
                     PAAEIAAPPAETAAPATGSAAPPAENVAPPAENVAPPVPATVAPGPEPGTAPSGPGPT
                     PAQAPEAPKSEPPSPPVTPPTPPGTPPPPPPAQQTPDPTPPMPQTQPQPPPS"
     gene            6608..7975
                     /gene="der"
                     /locus_tag="VFB37_16360"
     CDS             6608..7975
                     /gene="der"
                     /locus_tag="VFB37_16360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZO24071.1"
                     /translation="MLPVIALVGRPNVGKSTLFNVLTGTRDAIVADVPGLTRDRQYGY
                     GRLGPMGYVVIDTGGLIENPTGIEAQMRAQTARAVEEADRLIFLADARAGLTPQDEYV
                     ARELRRSGKPVTLALNKAEGLDTDAVSAEFHALGLGEPLTLSASHGEGCEQLIEHVLA
                     GIEPPQSDGPPADAIRVAIVGRPNVGKSTLVNRLLGEERVIASDAPGTTRDSILVPFR
                     RDNQDFVLIDTAGLRRRSKVQDIVERASVAKALQAIDEAHVVVLVLDAHDTIAEQDAS
                     VLGIALERGRALIIAVNKWDGIAAEQREQIHRQLALKLDFVPFVPLQFISARHGTGVG
                     ELAQLAVRAFEGAMREMPTRELTRTLEQAMTTHQPPMVHGRRIKLRYAHQGGKNPPRI
                     VIHGNQTAAVPDAYRRYLANVFRKRFDLFATPVAIEFRTDLNPYARRKPPPEKRSRRR
                     SAARR"
     gene            complement(7958..8725)
                     /locus_tag="VFB37_16365"
     CDS             complement(7958..8725)
                     /locus_tag="VFB37_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO24072.1"
                     /translation="MQLPEGSLVPGGVLIAPVEGPADRAPVVTFDGNRVMVLRSQDHW
                     VAIAGIPLTQKPGHIKLMVQSGDVAPTPRDFTVVDKHYEVQALKVAPSKVDLSAKDLA
                     RVGRESSRIHTALETFSSSPPATLRLIQPVPGIRSSSFGLRRVFNNEPRSPHAGMDIA
                     APTGTPIHAAADGRVIEAGNFFFTGNMVIVDHGEGLITMYGHMSKIDVQRGARVKAGE
                     ILGKVGATGRVTGAHLHFGVVLNSVSVDPALFLPPGG"
     gene            complement(8843..10210)
                     /gene="xseA"
                     /locus_tag="VFB37_16370"
     CDS             complement(8843..10210)
                     /gene="xseA"
                     /locus_tag="VFB37_16370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZO24073.1"
                     /translation="MPPSTQAGAEPERNVYTVSRLNREVRVLLERGLAVLWVQGELSN
                     LSVPASGHWYFSLKDRNSQLRCAMFRQRNMAAGFTPKAGQQVLARGRVSLYEPRGEFQ
                     FIVDHLEEAGVGALKAQFERVKAKLAGEGLFAAERKRALPRFPRRIGVITSPTGAAIR
                     DILHILSRRFPPAAVLIYPTPVQGAAAAASLVASLDLASARRECDVLILARGGGSLED
                     LWCFNDERVARAIHASAIPVVSGIGHEIDFTIADFVADARAPTPSGAAELVAPDRHAC
                     LEALTRTLERLSACMRRELRAVRSRFDGAGLRLKLAHPGVRLNQQQQRLDDLEQRLTG
                     AVRGALHSDRVRISEMFTRLLHESPAHAVREFRLRHEALAARLSHSMTECLSRAQHRF
                     DIATRTLDTVSPLATLSRGFAVVRRVADGKLLTDADRVAVGEDIEAQLAHGLLRARVT
                     QKGSS"
     gene            10310..>10593
                     /locus_tag="VFB37_16375"
     CDS             10310..>10593
                     /locus_tag="VFB37_16375"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZO24074.1"
                     /translation="MRILEEALTFDDVLLVPAYSEILPREVDLSTQVTRRIRLNVPML
                     SAAMDTVTEARLAITIAQEGGIGVIHKSMSIEAQAREVGRVKKFESGVIK"
BASE COUNT         1864 a         3512 c         3527 g         1690 t
ORIGIN      
        1 aaggccgctc ccggcacgat ccgcgcagat catgccctga gcatcgagca gaatgtcgtg
       61 cacggctccg atgccgccga caccgccgcc accgagattg cctatttctt tcgcgcgacg
      121 gagctgcacc cacgcggctg agcggccggc cctgtcatga acgcctcccc gtctccgctc
      181 gccgccaaac ccaccaacct gctggggttg ccacgcccgc agctcgagtc tttggtggcg
      241 gagctcgatg gcaagcctta tcgcgcccgt cagctgatga gctggatcta caagcgcggc
      301 gtggcatcga tgcacgagat gaccgacctg gccaagagcc tgcggacggt gctcgctgag
      361 cgcgccgagg tccgcacccc acagatcgtc acgaagcacg tctcgacgga tgacacgtgc
      421 aaatgggtgt tgcgagccga tgagtctcag gcgttcgaga tggtattcat ccccgagccg
      481 gaccgcggca cgttgtgcat ctcttcgcag gtgggttgcg cactggactg ctcgttctgc
      541 tccacggctc agcagggatt caaccgtaat ctgaccactg cggagatcgt cggacaggtc
      601 tggctcgcca agcagcagct gggtgagtac caggccgccg agactgctgc gcagggggcg
      661 cgtgcggcag tcggcggcga tgcacgaagg gtaaccaacg tcgtgctgat gggcatggga
      721 gagccgctgg ccaactttcg caacgtggtg gcggcgttac acatcctgct cgatgactac
      781 ggattcgacc tgtcgcgccg ccgggtgacg ctgtccacct cgggactcgt gccgcagatc
      841 taccggctgg ccgaggagac caacgtcgcc ctcgcagtct cgctgcatgc ccccgatgat
      901 gagctgcgca gccagctggt gccgatcaac cgcatccatc cgatcgctga gctgctggag
      961 gcctgctggc actacatcga cgagcagaac ggacgcagcg tgactttcga gtacgtcctg
     1021 ctggatgggg tcaatgactc gccggcgcag gcacgcgcgc tggcacagcg actgcgcggc
     1081 catccggcca aagtgaacct gatcccattc aatcccttcc cggggaccca ctatcggcgc
     1141 tcggcgatgc cggcgatcca gcgcttccgc gacgagttga ttcaacgcgg cgtactcgct
     1201 accgtccgcc gcacgcgcgg cgatgatatc gatgcggcgt gcggacagct tgccggacgg
     1261 gtcatcgacc ggaccacggt ccggctcggg agcaaggtat tcggggtggc ggtgcagtcg
     1321 tgaccacagt agcggccaga atgtccgggc ggtgccgcgc ggccgcgctc tgcggagcgc
     1381 tgctcgcgct cgggttgggc ggctgttcct cgcagcacgc caagcagaag cacgatgccg
     1441 ctaactacaa catgcagctc ggcatggctt acctgaatcg gggcgagctg gcgctggcga
     1501 aggacaagct cgagcgggcg ctgaaggaga atcccgagga tcccaacgtg cacagcgcga
     1561 tggcgatgct gcaggatcgc ctcggcaacg cggacaaggc ggacgccgaa ttcaaggccg
     1621 cgctgcgtct ggggccgagg gagccggata tcctgaacaa ctatgccgtg tatctctgcc
     1681 gaaccggccg caccgatgaa ggcgtgaaga gtttcgagga ggcggcgcac aacccgctgt
     1741 atcgcactcc ggaggtggcc tattcgaatg caggagtttg tctgcgtgcg gccaagcggg
     1801 acacccaggc tgcgatgagc ttccagcagg ccctgcagat caagcctaac ttcgccgaag
     1861 ccgcttacca gctcggcgag ctcgatttcg agcgcggcga gctcaacgag tcgcgcgatg
     1921 aggtcgatcg ctacctcggt gccttcgagg ccacgcccga tcttctcctg ctgcgcgtac
     1981 gaatcgcgcg caagcagggg gaccggctct ccgcggagcg ctacagcagc cagctgcgcc
     2041 gcgattttcc gggctcggcc caggcaagag tgcttgccgg gctcggccgc gactcggggt
     2101 gagccgatgg ccggggacgc tgccgggatt ggcgcgcggc tgcgggcggg ccgggagaaa
     2161 ctcaacctga ccgtgctgca ggctgccgag cgcatgcacg tcgatccgcg ccttctcgag
     2221 tcgctggagg cggaggattt cgccgctctc ggggctcccg tctatacgcg cggacatttg
     2281 cgcaactacg cggaactggt cggggaatcg gccccacagc tgagcgagct gctctcgcgg
     2341 gtggagctcg aggggcagcc ggatctgaca cgcatcgcca aggcgccccc ctcggtcgag
     2401 tcgagcaagc tgatcctgcc ggctctgttc gtgctggccg tgttcgcagt ggccggggca
     2461 gtgtggtggg tgctgtcgct ggcgccccag cagcccatac cgacgaacga gctctcggct
     2521 ccaccgaatg ccgatcgggc cagtcccagc ggttcggggg gcgcatctcc ggcaatccgc
     2581 cccggcgcgg tcccgccgca tgcggcggtg ggtcccacgc acctcgcctc ggcaggcggc
     2641 cccattacgg tgccgagcgg gacgcgcggc gcggtgagcg tcacgcagat ccccgacagg
     2701 ggttcttccg cgacggcccc gcccagtact gcgctgcccc ctcagccgga ctccgcgccc
     2761 gcaccccatc cctctcgcga gcagcaggtg actttgcatt attcggcgga ctgctggacg
     2821 gaggtgtatg actcgagtgg ccagcgtctc tactacgatg ttggagccgc tggcagcacg
     2881 cgtacgctga aaggcacggc acctctgcga gtcgtgctgg ccaacgccgc cggtgttgcg
     2941 gtcgaggtcg acgggcgcgg cacctccatt gccagaatgg cgcaaccgga tggaagcgcc
     3001 gaattcctcg tcaaccgctc cggtcgcgtg gcccgcactc gcgccccctc cagcggagac
     3061 tgagcttgcc ggatcagatc cagcccgttc gcgggatgaa cgacatcccg ccggcggaga
     3121 tcgcggcctg gcagttcctc gagcgctgca tcgccgagct cgtgaccgca tacggctacg
     3181 aggagatgcg cgtgccgctg atcgagcaca ccgcgctctt caagcgcgcg atcggcgagc
     3241 tcaccgatgt ggtgggaaag gagatgtata ccttcgagtc gcaaggcggc gagagcctga
     3301 cgttgcgccc ggaagcgacc gccggaatgg tgcgcgcgct catttccaac cgcatgctcc
     3361 gcggccaacg gcacaagctc tggtgcatag gtccgatgtt ccgccatgaa acgccacaag
     3421 ccggccgcta ccgtcagttc caccagctcg atgtcgaagc cgtcggtttc accggtcccg
     3481 atgtcgatgc ggagctgatc gccttgagcg cacgattgtg gcgcctgctc ggcatcgagc
     3541 acgtgcggct gcaggtcaac tcgctcggca cgcccgagtc gcgccgcgcc tatcgcgagc
     3601 tgctggtggg ctactttcgc gaccatgagg cgcagctcga tgccgacagc cgccgccgcc
     3661 tgggcggcaa cccgctgcgt attctggaca gcaagagtcc gcagatgcag gcgatcgtcg
     3721 cgggcgcacc gctgctgacg gagcacctgg atccgcagtc ccgcgagcat ttcgaggctt
     3781 tctgcgccct gctgcgcgat ttcggcgtgg agtacgagat caatccccgt ctggtccggg
     3841 gcctggacta ctacacccga accgtgttcg agtggatcac ggaatcctcc ggagcccaga
     3901 atgccgtatg ctccggcggc cgctacgatg ggctcatcgc gcagctcggc ggggagccca
     3961 cgccggccat tggctttgcg ctgggcatgg agcgcgtggt ggggctgctg gtgcaagcgg
     4021 gccgtgcgcc gccgccagcg aaaccggatg tgttcgtgat cctcagcggc gatcgcgccg
     4081 ggacggctgg gctcgggctg atcgagcggc tgcggaacga gctgccgcac cggcgcttcg
     4141 agtgcaatct ggggggcggc aatctcaaga cccagttcag gcgcgccgat cgcagcgctg
     4201 cgccgctcgc attgatcgtg gccgatgatg agctggaccg cggggtcgtg gcgatgaaac
     4261 ctttgcggca ggactccggt cagagcgagt gtccgattgc ccagcttgcg gcaggcatcg
     4321 agcaggcgct ggcggcgttg ggcgtttcgt agttccctgg ggggcgcgtg gaagagtatc
     4381 tgtccgaaaa agaacagtgg gaagccatca aggtctggct caaggagaat ctcctgtgga
     4441 tcgtcgccgg gatcgccgtc ggagctgcgg ggctgagcgg ctggcgctgg tatcaggcac
     4501 acgtgaacga ggtggccatc caggcgagca gccgctacga gcaactgttc gatgccgtcg
     4561 gaaagggcga tcgcgcccgg gcgctggttc tgctgggcga gctggaacgc gattacagct
     4621 cgtcccccta cctcgatcag gccaggctgc tggtcgcgcg ggcctatgtc gagacccatg
     4681 atctcgacaa ggcagccggc gagctgcagg cggtcgccgc acatacgaag gactcggagg
     4741 tcgcgatgat cgcccggctg cggctcgcgc gggtgcagat tgcgcagcag aagcccgatg
     4801 ccgcgctcac cactctgaat ggcatggagc ccggcgcctt tgcctggcgc tatcacgagg
     4861 tgcgcggcga tgcctactac gccaagggcg acaaggccaa cgccctgaag gaatacctga
     4921 gcgccaagac ggccgatatg ggcggcggac ccgacggtgc ggatctggat ctcaagatca
     4981 gtgacctgtc ggaccaaact ccccatctgg ccggcccgtc actggcgaca ccggccacgg
     5041 ccgccacctc caagtgagct catgagaaag gcgggttcga tgcgaagcag gtcatggcgc
     5101 gggacggtgg cggcactggg catgctcggg ctgttggcgg cctgctcgaa agataaggac
     5161 atcgatcagc cggcgccgct ggtgaccttc aattccacgt tgaaagtgga aagagcctgg
     5221 agcaacgccg tgcccgacaa gggcggcaag gtcttgcgtc tgggtctggg actcgctgtc
     5281 gagggcaacc gggtctttgc cgcgggacat cgtggtgaga tcgccgcctt cgatctgcac
     5341 agcggacatg tcctgtggcg caccaagagc aaggaagcct tctccggcgg ccccggagct
     5401 ggcgcgggac tcgtgatcgt cggcgcgagc accggtgaca tcctcgccgt ggacgaatcc
     5461 aacggcgcca cgcgttggaa agcgcggctc aacggcgagg tgctttccgc ccccgccgtt
     5521 tcggagaagc tcgtagccgt ccgcaccgtg gatggcaagc tgcacggcct ctcacccaag
     5581 gatggacacg agctctggag ccaggaggag caggtgccac gcctgtcgct gcgcgggacc
     5641 tcgcacccag tcatgacggg tgagctggtt ctgtgcgggt tcgacaacgg caaggtcgcg
     5701 gccgtcaaca tgaatgacgg tagcgtccag tggtcgacca cgattgcccc gccccacggc
     5761 aagaccgagc tggagcggct ggatgacgtg gacgctgcgg tgagggttgc aggtgcggat
     5821 gtctacgcag ttggattcca gggtcgcatc gcgatgctgg cactcgacac gggtcagatc
     5881 tggtggtcgc acgatgcttc cagctaccgc gggctcgggc ttgatgacag tgcgctgtat
     5941 gtctccaccg cagacggcga aatcgtcgct ctgaggcgcc gcacggggac ggagatctgg
     6001 cggcagaagg cactgctgca tcgccgactc tcggctgccg tggagactga aaacgccatc
     6061 gtgaccgggg attttcaggg ctacgtccat tggctgaaca aggcgaccgg cgccattgag
     6121 gcgcgtgcgc gaaccggcaa ggtgcgcatc agtaatccgc ctctggccgt gggcaacatg
     6181 gtgctcgtgg tgaacgacga tggccacatc acggccttcc gcacgacgcc tctgggcggc
     6241 ggttcgcgtg gcaagccagc cgccgagatc gccgcaccgc ccgccgagac cgccgcaccg
     6301 gcgacgggaa gcgccgcacc gcctgctgag aatgtcgcgc ccccggccga gaatgtcgcc
     6361 ccgcccgtgc ccgccacggt ggcccctggc cccgagccag gcactgcgcc gtccggtccc
     6421 gggccaacac ccgcgcaggc tcccgaggcc cccaagtccg agccgccgtc gccgccggtg
     6481 acaccgccaa caccgcccgg cacaccgccg cctccgccgc ccgcacagca gacgccggat
     6541 cccacaccgc cgatgccaca gacgcaaccg caacccccgc cctcctgagg agggatcggc
     6601 gccgcccatg ctgcctgtca tcgcgctcgt cggccgcccc aacgtcggca agtccacgct
     6661 gttcaatgtt ctgacgggca cgcgcgatgc aattgttgcg gacgtgcccg gactgacacg
     6721 ggatcgtcag tatggctacg gtcgcctcgg gccgatgggc tacgtggtga tcgacaccgg
     6781 tggcctgatc gagaatccaa cgggcatcga ggcgcagatg cgtgctcaga cggcgcgcgc
     6841 ggtggaggag gccgaccggc tgatatttct cgcggacgcc cgagcaggcc tgacaccgca
     6901 ggatgagtac gtcgcgcgtg agctgcggcg ctccggcaag cccgtcacat tggctctcaa
     6961 caaggcggag ggcctggaca ccgatgccgt gtcggccgag tttcacgcac tgggtctggg
     7021 agagcctctg accctgtccg ccagccacgg cgaaggatgc gagcagctca tcgagcacgt
     7081 gctggccggc atcgagccgc cgcaatccga tggcccgccg gcggatgcca tccgtgttgc
     7141 catcgtgggg cggccgaacg tcggcaagtc aacactggtc aatcgactgc tgggtgaaga
     7201 gcgcgtgatt gccagcgatg cgccgggcac tactcgcgac agcatcctcg ttcccttccg
     7261 gcgcgataac caggatttcg tcctcatcga cacggcgggg ttgcgccggc gctccaaggt
     7321 gcaggacatc gtcgaacgcg ccagcgtcgc caaggctctg caggcgatcg acgaagccca
     7381 tgtggtcgtt ctcgtgctcg atgcgcacga cacgatcgcc gagcaggatg cgagcgtgct
     7441 cggcatcgcc ctggagcgcg ggcgcgcttt gatcatcgcc gtcaacaagt gggatgggat
     7501 tgcggcggag cagcgcgagc agatccaccg gcagctcgcg ttgaagctgg atttcgtgcc
     7561 cttcgtgccg ctgcagttca tctctgcacg gcatggcacg ggcgtgggtg agctggcgca
     7621 gctcgccgtc agggccttcg aaggcgccat gcgcgaaatg ccgacccggg agctgacccg
     7681 cacgctcgag caggccatga ccacgcacca gccccccatg gtgcatggcc ggcgcatcaa
     7741 gcttcgatac gctcaccagg gcggaaagaa tccgccgcgt atcgtgatcc atggaaatca
     7801 gacggccgcg gtgccggatg cctaccgacg ctatctggcg aacgtgttcc gcaagcgctt
     7861 cgacttgttt gcaacaccgg tagccatcga gttccggacg gacctgaacc cgtacgcgcg
     7921 ccgcaagcct ccgccggaaa agcgctcgcg acgcaggtca gccgcccggc ggtagaaata
     7981 gcgccggatc gacggacacc gagttgagca ccacgccgaa atgcaggtgc gcccccgtga
     8041 cccgtccggt cgcaccaacc ttgccgagga tctcgccggc cttgacccgc gcaccgcgct
     8101 gcacgtcgat cttgctcatg tgaccgtaca tcgtgatgag cccttcaccg tgatcgacga
     8161 tgaccatatt gccggtgaag aagaagttgc cggcctcgat gacccggcca tcggcggcgg
     8221 cgtgaatggg cgtgccggtg ggcgccgcga tatccatgcc agcgtgagga ctgcgcggct
     8281 cgttgttgaa gacgcgtcgc agcccaaatg agctcgagcg gatccccggc accggttgga
     8341 tcaggcgcag ggtggcaggc gggctgctcg agaaggtttc gagtgccgtg tgaatgcgtg
     8401 agctctcacg gccgacgcgc gcgaggtcct tcgcagagag gtcgaccttc gagggggcca
     8461 ctttcagtgc ctggacctcg tagtgcttgt ccacgaccgt gaaatcccgg ggcgtcgggg
     8521 ccacatcgcc ggattgcacc atcaacttga tgtgtcccgg cttctgcgta agcggaatcc
     8581 cggcgatggc cacccagtga tcctgcgatc gcagcaccat cacgcgattg ccatcgaagg
     8641 tcacgacggg tgcccgatcg gcagggccct ccaccggagc aatcaacacg ccacccggaa
     8701 ccaggctgcc ttcaggtaac tgcaaggcac tctgcgcatc ggccgccacc gccgacgcgc
     8761 atgaacccca gcccaacgcg caaagcaaaa cctgcgccgt aagcgcacat cctcgcgacg
     8821 gaccgtcttt cgctgccccc atctaactcg accccttttg cgtcacccgt gcccgcaaca
     8881 aaccgtgcgc aagctgcgcc tcgatatcct cgcccaccgc cacccgatcg gcatcggtca
     8941 acagttttcc gtccgccacg cgcctcacca cggcgaagcc ccgcgacagc gtcgcaagag
     9001 gactgaccgt atccagcgtc cgagtcgcaa tgtcgaagcg atgctgcgcc cgcgacagac
     9061 actccgtcat ggaatgtgag agccgggcag ccagcgcctc gtgtcgcaag cgaaattccc
     9121 gcacggcatg tgcgggagac tcgtgcagca ggcgcgtgaa catctcgctg atgcgcacgc
     9181 gatcactgtg cagcgcgccg cgcacggcgc cggtgagccg ttgctcgaga tcgtccagcc
     9241 gctgctgctg ctgattcaag cggacaccgg gatgagccag cttcaggcgc aaacccgccc
     9301 cgtcaaaccg tgacctcacc gcccgcagct cgcgccgcat gcacgcgctc agccgttcca
     9361 gcgtccgagt cagagcctcc agacacgcat ggcgatccgg agcgaccagc tcagcagcgc
     9421 cggaaggagt cggtgcgcgg gcatccgcca cgaagtcggc gatggtgaag tcgatttcat
     9481 ggccgatacc ggacacgacg ggtatcgccg atgcgtgaat ggcgcgcgcg acgcgctcat
     9541 cattgaaaca ccacaggtcc tccagcgagc cgccaccacg ggcgaggatc aagacatcgc
     9601 actcgcgccg tgcggaggcg agatcgaggc tcgctacgag cgaagcagcc gctgccgcgc
     9661 cctgcacggg ggtcgggtag atgagcacgg cagcaggcgg aaaccttcga ctgaggatgt
     9721 gcaggatgtc acgaatggca gcgcccgttg gcgaggtgat gacgccgatg cgacgtggaa
     9781 agcggggcag ggcgcgcttg cgttccgccg caaacagtcc ctcgccggcg agcttggcct
     9841 tcacgcgctc gaactgggct ttcaacgcgc cgacgccggc ttcctcgagg tgatcgacga
     9901 tgaactgaaa ctcgccgcgc ggctcgtaca agctcacgcg cccgcgcgcg agcacctgct
     9961 ggccggcctt cggcgtgaag cccgccgcca tgttccgctg acgaaacatc gcgcagcgca
    10021 gctgcgaatt gcgatctttc agcgagaaat accagtggcc ggaggcaggc accgagagat
    10081 tcgagagctc gccctggacc cacaggaccg cgagcccgcg ctccagcagc acacgcacct
    10141 cgcggttcag gcgggagacg gtgtagacgt tgcgctcggg ctcggcgccg gcctgggtgc
    10201 tgggcggcat gggcggaaac catatcacgg gtttaccctg aagcggttcc cgcgtacaat
    10261 ttcacgcttt atcttcaccg agtgcgactc gagcgacacc tggcattgca tgcgtatcct
    10321 cgaagaagcg ctgaccttcg atgatgtcct cctggttccg gcgtattcgg aaatcctgcc
    10381 acgcgaggtc gatctctcca cccaagtcac gcggcgcatc cggctgaacg tgccgatgct
    10441 ctctgcggcg atggacaccg tgaccgaggc gcgcctcgcc atcacgatcg cgcaggaagg
    10501 cggcatcggc gtcatccaca agagcatgag tatcgaggcc caggcgcgcg aagtgggccg
    10561 cgtgaagaaa ttcgagagcg gcgtgatcaa gga
//
LOCUS       DASRHN010000403         6697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_638408, whole genome shotgun sequence.
ACCESSION   DASRHN010000403 DASRHN010000000
VERSION     DASRHN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638408"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFB37_16380"
     CDS             complement(<1..502)
                     /locus_tag="VFB37_16380"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZO24075.1"
                     /translation="MLTAAATLAVTRNWRLLAPSMSTSLPSLTKPALGAAASATPAHR
                     RWIGVGLAAAVAGAGTVAAALPWAHTLGLSALTLSIVLGILVGNTVFPRIAPWAGPGV
                     DFCKNTLLRAGVILYGFRISFGQLAEIGWAGVAIDIVMMTLVFTIAVRLGPRLFKLDR
                     DTALLIG"
     gene            544..1479
                     /locus_tag="VFB37_16385"
     CDS             544..1479
                     /locus_tag="VFB37_16385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZO24076.1"
                     /translation="MQLNLHLLRIFFTVAAQKSFSTAAGVLRVSQPAVSKAVRELERQ
                     LDLPLLERSAAGARGVQLTANGAALFEHARGIFALERAATEDIRARIGLQRGRLSIGA
                     STTVAGYWLPPYVAAFSRRFPAIDLKIGVGNTQTVCDGLIGCGFDIALVEGAIQDSRI
                     LATHWRDDELRIIASPAAAVARRRRPVLAELEEQVWLLRETGSGTREVTERLMRAHGI
                     SPRRTVEVGSNEGIARAVAAGLGIAMLPTCLLGELITLQQVKALSFPDDSALVRPLLL
                     LQLKERAPSPLLRAFREVLQQAPDTAHEAAPRVRR"
     gene            1534..3063
                     /locus_tag="VFB37_16390"
     CDS             1534..3063
                     /locus_tag="VFB37_16390"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO24077.1"
                     /translation="MAETGSFDPGRREFVQRAASGVALAATLPAMALAASRDSDKAAV
                     LARIPGMHADNVRRLQEWIALPSIAAENRNYPQGPEYMATLARNAGFTDVKLIPTAGK
                     PGVFGKLDAGARTTMGLYFMYDVKQFVPAEWSSPPLEGRLVQKEGLGTVCMGRGAVNQ
                     KGPQNSFLSALQAFKAAGKELPINLVLVCEGEEEIASPHFREIVTHPEVMSELKKCAG
                     IFMPEAAQDRDGGVQVSLGAKGVVELELVSSGEKWGRGPAHDVHSSLEAQVDSPSWHL
                     VQALNTLIEADGHTPAVEGFFEKAKPLTAAQEQMVRVHAAKTAESTVKQVLGVQRWVH
                     DANWVDSLLLLESKPTINIEGLVGGYTGPGGKTVLPHRAVAKIDMRLVPDMTAEDTLA
                     KLKSHLAKHGFGDIEVNMSGGYDPTQTDPASRLIQAQLATYRKLGFDPQLWPRSAGSW
                     PGYVFTDAPLRLAAGHFGLGHGTGAHAPNEYYLIESTNPKVRGLDGAVASFVEYLYAL
                     A"
     gene            3149..3760
                     /locus_tag="VFB37_16395"
     CDS             3149..3760
                     /locus_tag="VFB37_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HZO24078.1"
                     /translation="MAQGVLFPTTGEVFATGFAYEPGFLSSEEEALLIGEIGKLPLAP
                     AEYKQYLARRRIVSYGGRYDYTARRLGQGEPIAPFLLPLRARAAAWIGCAAESLTHAL
                     VAEYAPGVPLGWHRDVPDFEFVIGISLLSDCRMRFRPYPPRPREKSIALELAARSIYS
                     LRGDARWNWQHSVPPVPALRYSITFRSQNGQRLLAHAGKSPCA"
     gene            complement(3813..6521)
                     /locus_tag="VFB37_16400"
     CDS             complement(3813..6521)
                     /locus_tag="VFB37_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZO24079.1"
                     /translation="MQERAIRSVLGALAAALIPLIAAAAQPMDEEFAQHVKQWTTKPE
                     FSSPLVDHLPVSQAVPSPKQVLGHDIGEPNKLDYYADLLKYYRALAAKSPRVRIVEIG
                     KTEEGRENVVVMLSSEENIRNLESNRANLARLADPRALSDEQASEIIRRTKPIYHFMG
                     GLHSAETGPAEMLLELAYRLVAEDSPLLDAVRENLVVAITPATDPDGRDRYTDWYYRN
                     KIDDTDDLNPVPGAPYWGKYIFHDDNRDINYSGASARELLAYYLQWHPPIMHDLHESV
                     PFLYSYSGQAPQNPNLDPILYGELPWFSNFEMSQLTKYGMPGVWTHGFVDAWSPGYVG
                     FMSSNHNGMLRFYETFGNGGATTMLRHVAPVPGEPKDPNRPDQTKREWFRPLPPYKEV
                     QWSMRNNTNYMETAALTALQLTSQFPQVVLQNFYRKSRNSIESGRTQAPYGYIFPADQ
                     PDMTRAQFVVNILRLQGIEVGRATQDIRLKEGSYPSGSLVVKRNQPYGRLAKMLLEKQ
                     QYPEGDLKTYDDTAWTMGLMAHTKVIESADPQLLDIPVEPVEHFEVRGDIGAAHATGY
                     AVLDDGSPNLATLRYRLKSVPIRIAEQSFQSAGRTVPAGSFLFEGRAYEKLKPEVESL
                     GLHAVALESKPDVPAHPAALPRIAVYSTWGSTQNVGWVRYTFDQFETPYDLIFKERLR
                     KGGLHSAYDIIIIPSQGRSGRSLVYDVEVHGKPLPYTRTSRYQYLGAYGSSEDIRGGM
                     GLEGLLELRKFVEAGGTLITLGDASSMPPDFDITPTIEVSHPTQKFYAPGPIVQAKIL
                     KPKNPIFYGYEGNDTNVRWASNALLQVKVRDKDRILMEFPGGEKSVLSGLMRGADEIE
                     NRPAIVDMPVGKGQVVLFATNPVYRWQNLGEFRMLYNSIFSYRNLQ"
BASE COUNT         1179 a         2134 c         2169 g         1215 t
ORIGIN      
        1 ctccgatcag cagagccgta tcccgatcga gcttgaagag gcgcgggccc aggcgcacgg
       61 cgatcgtgaa gaccagcgtc atcatcacga tgtcgatggc aacgcccgcc catccgatct
      121 cggcgagctg gccgaagctg atgcggaatc cgtagaggat cacgcccgcc cgcagcaggg
      181 tgttcttaca gaaatccaca ccgggtccgg cccacggcgc gatgcgcgga aaaaccgtgt
      241 tcccgaccag gatgccgaga acgatcgaca gcgtgagcgc gctgagcccc agcgtgtgag
      301 cccagggcag cgccgctgcc acggtgcccg ccccggcgac cgcggcggcc aggccaaccc
      361 cgatccaccg ccggtgcgca ggagtcgcag atgctgccgc gccaagggct ggcttcgtca
      421 ggcttggaag gctcgtgctc atcgaaggcg ccaggagtct ccagttgcgc gtgactgcca
      481 gcgtagcggc cgccgtgagc atgagtacaa tacataaatc tcacatttcc atgaatttaa
      541 gttatgcagc tcaatctgca cctgttgcgc atctttttca ccgtagccgc gcagaaaagc
      601 ttctcaacgg cagcaggcgt gctgcgggtg agtcagcccg ccgtgtccaa ggccgtgcgc
      661 gagctggagc gccaactcga tctgcccctg ctggagcgct cggcggccgg tgcccggggg
      721 gtgcagctca ccgccaacgg ggccgcgctc ttcgagcacg cgcgcggcat ctttgcactc
      781 gagcgcgccg ccaccgagga catccgggcg cgcatcggac tgcagcgcgg acggctctcg
      841 atcggggcga gcacgaccgt cgcaggctac tggctgccgc cttatgtcgc cgccttctcg
      901 cgcagatttc cggcaatcga cctcaagatc ggggtcggca acacacagac cgtgtgtgat
      961 ggactgatcg gatgtggatt cgacatcgcg ctggtggaag gggcgatcca ggattcacgc
     1021 atactcgcca cgcactggcg cgatgatgag ttgcgcatca tcgcctcgcc agcggcggct
     1081 gtggcacgcc gccgccgacc cgtgctggcc gagctcgagg agcaggtctg gctactgcgc
     1141 gagaccggct ccggtacgcg cgaggtgacc gaaaggctga tgcgcgccca cgggatctcg
     1201 ccccgacgca cggtcgaggt cggcagcaac gaaggcatcg cgcgggcagt cgctgcgggc
     1261 cttggcattg cgatgctgcc gacctgcctg ctcggagagt tgattaccct gcagcaggtg
     1321 aaagcactga gcttccccga tgactcggct ctcgtacgtc ccctgctgct gctgcaattg
     1381 aaggagcgcg caccctcacc gctgttgcgt gcgtttcgcg aggtgttgca gcaagcgcct
     1441 gacacggccc acgaggctgc tccgcgcgtg cgccgatgat agtattcgcc gcctgattca
     1501 aacccttgta ccgctcgctg aagggagagg cgtatggccg agacaggcag tttcgatccg
     1561 ggcaggcgtg aattcgtgca acgagctgca tccggtgtgg ctctcgctgc gacgctgccg
     1621 gccatggcgc tcgcggccag tcgcgattcg gacaaggccg cggtgctggc gcggatcccg
     1681 ggcatgcatg cagacaatgt ccggcgcctg caggagtgga tcgccctacc ctcgatagcg
     1741 gccgagaacc gcaactatcc tcagggcccg gagtacatgg ccacgctcgc gcgcaatgcg
     1801 ggcttcacgg acgtcaagct catccccacg gcaggcaagc cgggtgtgtt cggcaagctc
     1861 gatgccggcg cccgcaccac tatgggactg tacttcatgt acgacgtgaa gcagttcgtc
     1921 ccggccgagt ggagctcacc gccgctcgaa gggcgcctgg tgcagaagga aggactcgga
     1981 accgtatgca tgggccgcgg tgccgtgaac cagaaagggc cccagaattc cttcctgagc
     2041 gccctgcagg cattcaaggc cgcgggcaaa gagctgccga tcaacctcgt cctggtgtgt
     2101 gaaggcgagg aggagatcgc ctcgccgcac tttcgtgaga tcgtcaccca tcccgaggtg
     2161 atgagcgagt tgaagaagtg cgccggcatt ttcatgcccg aagccgctca ggaccgcgac
     2221 ggcggcgtgc aggtttccct tggcgccaag ggcgtggtgg agctcgagtt ggtttcgagc
     2281 ggtgagaagt gggggcgcgg tcccgcacac gatgtgcact cgagcctcga agctcaggtg
     2341 gactcgccca gctggcacct ggtgcaggcc ttgaacaccc tcatcgaggc ggacggccat
     2401 acccctgcgg tggaaggctt tttcgagaag gcgaagccgc tgaccgccgc ccaggagcag
     2461 atggtccgcg tgcatgcggc gaagaccgcc gagagcaccg tcaagcaggt gctcggtgtg
     2521 cagcgctggg tgcacgatgc caattgggtc gattccctct tgttactcga gtccaagccg
     2581 acgatcaaca tcgaagggct ggtcgggggc tacaccggtc ccggcggaaa gacggtcctg
     2641 ccgcaccggg ctgtggcgaa gatcgatatg cggctggtac cggacatgac cgctgaggac
     2701 accctcgcca agctcaagtc ccatctcgcc aagcacggct tcggcgacat cgaagtcaac
     2761 atgagcggcg gctacgaccc aacccagacc gatcccgcca gccggctcat ccaggcccaa
     2821 ctggcgacct atcgcaagct cggcttcgat ccgcagctgt ggccgcgctc ggcgggctcc
     2881 tggcccggat acgtcttcac cgatgcgccg cttcgcctgg ccgccggtca tttcggactc
     2941 ggacacggga ccggtgcgca tgccccgaat gagtactacc tgatcgagtc gaccaacccc
     3001 aaggtgcgcg gactcgacgg tgccgtcgcc tcgttcgtgg aatacctgta cgcgctcgct
     3061 tagcgagctg acgtagcgag ccattcgccc gggtccgccg gcatcgagcc cctattgtgc
     3121 cgcggcaaag cgccctcatg aaggtccgat ggctcagggt gtgctgtttc ccacgaccgg
     3181 ggaggtattc gcgacgggct tcgcttatga gcccggcttc ctctccagtg aggaggaggc
     3241 tctcctgatc ggggagatcg ggaaactgcc tctcgcgccg gcggaataca aacaatatct
     3301 cgcgcggcgg cgcatcgtga gctacggcgg acgatacgac tacactgcgc gccgcctcgg
     3361 gcaaggcgag cccatcgcac ctttcctgct gccactacgc gcgcgggccg ctgcctggat
     3421 cggatgcgcc gccgagagcc tgacgcacgc gctcgtcgcc gaatacgctc ccggtgtccc
     3481 actgggttgg caccgtgacg tcccggattt cgagttcgtg attgggatct cccttctgag
     3541 cgactgccgc atgcgcttcc ggccctaccc accccgcccg cgcgagaaat ccatcgcgct
     3601 cgagctggcg gcgcgctcga tctacagcct tcgtggggat gcgagatgga actggcagca
     3661 cagtgtgccg cccgtaccgg ctctgcgtta ctccatcact ttccgctccc agaacggtca
     3721 gcgactactg gcacacgcgg ggaagtcccc ttgcgcctga gccctcttcc aggggatgac
     3781 ccgcgtgtga caatctccgg gaccggaccg gctcattgca ggttccggta gctgaaaatc
     3841 gagttataga gcatccggaa ctcaccgaga ttctgccatc gatagacggg gttagtcgca
     3901 aacagcacca cctgaccctt ccccaccggc atatcaacaa tcgccgggcg attctcgatc
     3961 tcatcggcgc cgcgcatcag cccactcagc acgctcttct ccccgcctgg gaattccatg
     4021 aggatccggt ccttgtcgcg caccttcacc tgcaggagcg cgttgctggc ccagcgcacg
     4081 ttggtgtcgt tcccctcata accgtaaaag atggggttct tcggcttgag gattttggcc
     4141 tgcacgatcg gaccgggtgc gtagaacttc tgcgtcggat gcgagacttc gatcgtaggc
     4201 gtgatgtcga aatcgggggg catcgagctg gcgtcgccga gcgtgatcag ggtcccgccc
     4261 gcctcgacga actttctcag ctccagcagg ccttccaggc ccatcccgcc gcggatgtcc
     4321 tcggaggagc cgtaggcacc gaggtactgg tatcgtgacg tgcgcgtgta gggcaacggc
     4381 ttgccatgaa cctccacgtc gtacacgagg ctgcgcccgg acctgccctg gctgggaatg
     4441 atgatgatgt cgtacgccga atgcagtccg cccttcctga gccgctcctt gaagatcaga
     4501 tcgtagggag tctcgaactg atcgaaggtg tagcgcaccc agcccacgtt ctgggtgctg
     4561 ccccaggtgc tatagacagc gattcgaggc agggcagccg gatgagcggg cacatcgggc
     4621 ttcgactcga gtgcgacagc gtgcagacca agcgattcga cctcgggttt gagcttctca
     4681 taggcccggc cttcgaacag gaacgaccct gcgggcacgg tgcggcctgc cgactggaag
     4741 ctttgctcgg cgatccggat cggcacgctc ttcaggcggt aacggagggt cgccaggttg
     4801 ggtgagccgt catccagcac ggcatagccg gtggcgtgcg cagcgccgat gtcgccgcgg
     4861 acttcgaaat gctccacggg ctcgacgggg atatcgagca gttgcggatc cgcgctctcg
     4921 atgaccttcg tgtgggccat gaggcccatg gtccaggcgg tgtcgtcata ggtcttgaga
     4981 tcgccctcgg gatactgctg cttctcgagc agcatcttgg cgagacgccc gtagggctga
     5041 ttgcgcttca cgaccaggga gccggacgga tagctgcctt ccttgagacg gatgtcctgg
     5101 gtcgcgcggc ccacctcgat cccctgcagc cgcaggatgt tcacgacgaa ctgggcccgg
     5161 gtcatgtctg gctggtccgc gggaaagatg tatccgtagg gggcttgcgt gcggccggac
     5221 tcgatggagt tgcggctctt gcggtagaag ttctgcagca cgacctgggg aaactgcgag
     5281 gtgagctgca aggccgtgag cgctgcggtc tccatgtagt tggtgttgtt gcgcatcgac
     5341 cattgcacct ccttgtaggg aggcagcggc cggaaccact cgcgcttcgt ctgatcgggc
     5401 cggttcggat ccttgggctc acccggcacg ggggccacgt gccgcagcat cgtggtcgcg
     5461 ccgccgttgc cgaaggtctc gtaaaaacgc aacatcccgt tgtgattcga cgacatgaag
     5521 cccacgtatc ccggcgacca ggcatccacg aagccgtggg tccacacgcc cggcatgccg
     5581 tacttggtga gctgcgacat ctcgaagttg gaaaaccagg gcagctcgcc gtacaggatc
     5641 ggatcgaggt tagggttctg cggagcttgc ccgctgtagg agtagaggaa cggcaccgac
     5701 tcgtgcaggt cgtgcatgat cggcggatgc cactgcaggt aatacgccag aagctcccgg
     5761 gcgctcgcgc ctgaatagtt gatgtcgcga ttgtcgtcgt gaaagatgta tttgccccag
     5821 tagggcgcac ccggcaccgg gttcagatcg tcagtgtcgt caatcttgtt gcggtagtac
     5881 cagtcggtgt accggtcgcg gccgtcggga tcggttgctg gcgtgatggc caccacgagg
     5941 ttctcgcgta cggcatcgag caaaggcgag tcttccgcga ccaaccgata ggcgagctcc
     6001 agcagcatct cggcaggccc cgtttccgcg ctgtgcaacc cgcccatgaa gtgatagatc
     6061 ggcttggtgc ggcggatgat ctcggatgcc tgctcgtcgg acagcgcgcg cgggtcggca
     6121 agccgcgcca gatttgcgcg attggattcc aggttgcgga tgttctcctc ggaggacagc
     6181 atcaccacta cgttctcgcg tccctcctcc gtcttgccga tctcgacaat cctcacccgt
     6241 ggggatttcg ccgcgagcgc acggtaatac ttcaacagat cggcgtagta atcgagcttg
     6301 ttcggctcgc cgatgtcgtg gccgagaacc tgcttcggag agggtacggc ctgcgacacc
     6361 ggcaggtgat ccaccagcgg gctggagaat tccggcttcg tggtccattg cttgacgtgc
     6421 tgcgcaaact cctcgtccat gggctgcgca gcggcggcga tcaagggaat cagcgccgcc
     6481 gcgagcgctc ccagaaccga tcgaatggca cgctcctgca tcgacgctcc cctacgctgc
     6541 tggcaaaagc ggggattatt gcccaaatcg gcatggggcg ttgcgccgcg cacggctcgc
     6601 accggattgg gcccgttcgg cgggcggggc acctcagcgc actattatct tgatatagct
     6661 tcaggtaggt tcaggtgcgg cgagcgccct cagctga
//
LOCUS       DASRHN010000404         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1133185, whole genome shotgun sequence.
ACCESSION   DASRHN010000404 DASRHN010000000
VERSION     DASRHN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133185"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2932)
                     /locus_tag="VFB37_16405"
     CDS             complement(<1..2932)
                     /locus_tag="VFB37_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZO24080.1"
                     /translation="MMQLVWIALLGAWTSASLARDPPQAGSQQPGPPAQQGPAQPLPV
                     QPPPTPPPAAAQQAAQVAQQAAAVEGNPDWAVTLERIAGSVVSIDVDLTRAFDTEWNS
                     TAQATGFVVDAERGLILTNRHVVTPGPVTAEATFLNREEVQLYPVYRDPVHDFGFYRY
                     DPAKLHFIKPKALALYPEGAQIGREIRVVGNNAGEQLSILAGTLARLDRDAPEYGIAK
                     YNDFNTFYLQAASGTSGGSSGSPVIDIRGRVVALNAGGASGAASSFYLPLGRVRRALE
                     LIQQGKPVTRGTLCAVFNYTPYDELKRLGLNPATEANVRKAYPQYTGMLVVTEVLPGS
                     PSESVLQPGDVLVRVNGHYVTQFEPLDEVLDSNVGGHVQVELERGGKSVSAKLPVTDL
                     HAITPAAYAEFGDAVLNTLSYQQARQFNVPVRGVYIANPGYVFAAAGIPRGAVITMMD
                     SKRVDSLADVEAHIAQLGNGDRTTIRYITIDDPNSSQLRSLRMDRRWFPAHHCDRNDR
                     EGLWDCHDLPSGPAPKPIKVSSTEFPQFKDPRLQALAPSLAMVTFDMPYSISGITERN
                     YHGTGLVVDAQRGLVVVDRNTVPVAVGDVTVTFAGTVQVPGRVVYIHPLHNLAVVAYD
                     PKLLGNTPVKSAKLAPSELTAGEPVWVVGLGADSTMHSRSTQIASIDPLELPLSRTMR
                     FRDSNLEAAQLVNPPTDFDGVLSDKGGNVLGLWSSFAYENGREVAQDNRGVPIELVGD
                     MLERVRNDKPLHSLEAEFNVQPLASAREFGLSEEWTHKLAEQNPTQRQALTVVRMVGG
                     SPASHALQQGDIVLSIDGKVVTRFREAERAAADKDHVNVVVWRGSGEQSLDVQTVVLP
                     GADIARLVEWAGATLQAPHRAMSAQRGIPPVGVYVGYFSYGSPATRYGLYPGRRIVEV
                     DGVPTPDLDAFLGAVTGRPDRSSVRLKTITWNNAPEVITLKLDKHYWPAYELVNTDAG
                     WERKA"
     gene            complement(3040..3426)
                     /locus_tag="VFB37_16410"
     CDS             complement(3040..3426)
                     /locus_tag="VFB37_16410"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZO24081.1"
                     /translation="MDADSAVVDRYKRRGALHVVRALGASWRGLRGAFREEVAFRQEL
                     AIAVLVIPMGLWLGHSGVERALLIGPMLLILVVELINSAIEATVDRIGFERHALAGLA
                     KDIGSAAVLMSFVLLGAVWLLILLGR"
     gene            complement(3402..>3674)
                     /locus_tag="VFB37_16415"
     CDS             complement(3402..>3674)
                     /locus_tag="VFB37_16415"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24082.1"
                     /translation="FAKRLLDHGFHAPTTYFPLLVPECLLIEPTETESKDVLDAFVTA
                     MAQIQKEAEQEPERVTGAPHTMPVRRLDDVRAAKQLDLRWTPTARS"
BASE COUNT          634 a         1178 c         1223 g          639 t
ORIGIN      
        1 aggccttgcg ctcccaaccc gcgtccgtat tcaccaactc gtaggcgggc cagtagtgct
       61 tatcgagctt cagcgtgatc acttccggcg cgttgttcca tgtaatcgtc ttgagacgca
      121 ccgaggagcg atcgggtcgg ccggttaccg cgccaaggaa ggcatcgagg tcgggagtcg
      181 gtacgccgtc gacttcgacg atgcggcgcc cggggtagag cccatagcgc gtagcaggag
      241 acccatagga gaaatagccg acgtagacgc ccaccggggg gatcccccgc tgggcgctca
      301 tggcgcgatg gggggcctgc aaggtggctc cggcccattc gaccagccga gcgatgtccg
      361 cgcccggcag caccacggtc tgaacatcaa gagattgttc gccactgccg cgccagacga
      421 ccacgttgac gtgatccttg tccgctgctg cgcgctcggc ttcgcggaaa cgggtaacca
      481 ccttgccgtc gatcgacagg acgatgtcac cttgctgcaa tgcgtgtgag gccggcgagc
      541 cgcccaccat ccgaacgacc gtgagtgcct ggcgctgtgt ggggttctgc tcggccagct
      601 tgtgggtcca ctcctccgac aatccgaact cgcgtgcgct cgcgaggggc tgcacattga
      661 actccgcctc gagagagtgg agcggtttgt cattgcgcac gcgctcgagc atgtccccga
      721 ccagctcgat gggcacgccc cggttgtcct gtgcgacctc gcggccattc tcgtaggcga
      781 agctggacca caggcccagc acgttgccgc ccttgtcgct cagcacgcca tcaaaatcgg
      841 tgggcggatt gaccagttgc gccgcctcga gattgctgtc gcggaagcgc atggttcgcg
      901 agagcggcag ctccagcgga tcgatgctgg caatctgcgt gctccgtgaa tgcatggtgc
      961 tgtcggcgcc gagcccgacc acccacaccg gctcgcccgc cgtcagctca ctcggtgcga
     1021 gcttggcaga cttcaccggc gtattgccga gcaacttcgg gtcataggcg accaccgcca
     1081 ggttgtgcag ggggtggatg tagaccactc gacccggcac ctggacggtg cccgcgaagg
     1141 tgactgtgac atctcctacc gcaacgggaa ctgtgttgcg atcgacgaca accagacctc
     1201 gctgtgcatc aacgacgagg ccggtgccgt gatagttacg ctcggtgatg cccgagatcg
     1261 agtacggcat gtcgaaggtc accatcgcca gcgagggcgc cagggcctgc aggcgcgggt
     1321 ccttgaactg cggaaattcg gtagagctga ccttgatggg tttcggcgcc ggccctgatg
     1381 gcagatcgtg gcagtcccaa agcccttcgc gatcattgcg gtcacagtga tgggccggga
     1441 accagcgccg gtccatgcgt agcgagcgca actgactgct gttgggatcg tcgatcgtga
     1501 tgtagcggat cgtggtgcga tcgccattgc cgagctgcgc gatatgcgcc tccacgtctg
     1561 ccagagagtc cacccgcttg gagtccatca tggtgatgac cgcgccgcgc ggaatgccgg
     1621 ccgcagcgaa cacgtaaccg gggttcgcga tgtagacgcc gcgcacgggc acgttgaact
     1681 gccgcgcctg ctggtacgag agggtgttga gtaccgcatc gccgaactcg gcatacgcgg
     1741 caggcgtgat ggcatgcagg tcggtgaccg gcagcttcgc cgacacgctc ttcccacccc
     1801 gctcgagctc cacttgaacg tggcctccga cattggaatc caggacttca tccaggggct
     1861 cgaactgggt cacgtaatgg ccgttgaccc gcaccagcac gtccccgggc tggaggacgc
     1921 tctcgctggg cgatccgggc agcacctccg tgacgaccag catgcccgtg tattgcgggt
     1981 aggccttgcg cacgtttgct tcggtcgcgg ggttcaggcc gagtcgtttg agctcatcgt
     2041 agggggtgta gttgaacacc gcacacagtg tcccgcgtgt cacgggcttg ccctgctgga
     2101 tcagctcgag cgcccggcgc acgcggccca gcggcaggta aaagctcgaa gcagcgccgc
     2161 tggcgccgcc ggcattcaac gccaccacgc ggccgcggat gtcgatgacc ggcgagcccg
     2221 aggagccgcc ggaggtgcct gaggcggcct gcagatagaa cgtgttgaaa tcgttgtact
     2281 tggcgatgcc gtactccggc gcatcacggt cgaggcgtgc cagggtgccg gcgaggatcg
     2341 acagctgctc gccggcattg ttgccgacga cccggatctc ccggccgatc tgcgcgccct
     2401 ccggatacag cgcaagcgcc ttgggcttga tgaagtgcag cttggcagga tcgtagcgat
     2461 agaagccgaa atcgtgcacg ggatcgcgat acaccggata gagctgcacc tcttcgcggt
     2521 tgagaaaggt cgcttcggcg gtcacgggtc ccggcgtgac gacgtgccgg ttggtcagta
     2581 tgaggccgcg ctcggcatcc accacgaaac ctgtcgcctg tgcagtggag ttccactcgg
     2641 tatcgaacgc gcgagtcaga tccacgtcga tggagacgac gctgccggcg atccgctcga
     2701 gcgtcaccgc ccagtccggg ttgccctcca cggccgcggc ctgttgtgca acttgcgcgg
     2761 cctgctgtgc agcagccggg ggcggggtgg ggggcggttg caccggcaag ggctgcgccg
     2821 ggccctgctg ggccgggggg cccggctgct ggctgcccgc ttgcggcgga tcgcgcgcga
     2881 ggctcgcgga agtccaggct cccagcagag cgatccacac gagttgcatc atgagtgtcg
     2941 tgacaagcgt cgcgaccgat ctgccggatc gggtgaatcg agtcgatgca acggcgcgcg
     3001 gcgcgccgga cagaagctct gcgtacatca attgcctatt taacggccga gcagaatgag
     3061 cagccagacg gcgcccagca ggacaaagga catgagcact gcagccgagc cgatgtcctt
     3121 ggcgagcccg gcgagcgcgt gacgctcgaa accgatccgg tccacggtgg cttcgatcgc
     3181 actgttgatg agctccacta ccaggatcag cagcatcgga ccgatcagca gggcgcgctc
     3241 gacacccgag tgccccagcc acagacccat cggtatgacg agcactgcga tggcgagttc
     3301 ctggcgaaaa gccacttcct cgcggaaggc gccccgcagc ccgcgccagc tggctcccag
     3361 tgcccgcacg acgtggagtg cgccccggcg tttgtagcga tctacgaccg cgctgtcggc
     3421 gtccatctca gatccagttg cttggctgcc cgcacatcgt cgagccgccg caccggcatg
     3481 gtatgcggtg cgccggtcac gcgctcgggt tcctgctccg cctccttctg aatctgggcc
     3541 atggccgtca cgaaagcatc caggacgtcc ttgctctccg tttctgtcgg ctcgatcagc
     3601 agacattcgg gcaccagcag cgggaaatag gtggttgggg cgtggaatcc atggtcgagc
     3661 aggcgcttcg cgaa
//
LOCUS       DASRHN010000405         9671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1306289, whole genome shotgun sequence.
ACCESSION   DASRHN010000405 DASRHN010000000
VERSION     DASRHN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306289"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /gene="yidD"
                     /locus_tag="VFB37_16420"
     CDS             <1..169
                     /gene="yidD"
                     /locus_tag="VFB37_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZO24083.1"
                     /translation="SCSQYAIEAIRCFGVGRGSWLTLKRLGRCHPWHPGGFDPVPTEH
                     GAMSCRRPAHD"
     gene            162..1892
                     /gene="yidC"
                     /locus_tag="VFB37_16425"
     CDS             162..1892
                     /gene="yidC"
                     /locus_tag="VFB37_16425"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZO24084.1"
                     /translation="MTNNPRLLLWLALIAMLWLNFVAWNRDYGERPQPASSQPAGAGS
                     SAAGAPSSVDLANQVPQAQKAPSSSATGQPAQPASPPTESSVSSPPAPTTASTELGSG
                     VVHVQTDVLDMDISTRGGTIERVDLPKYPQVKGQAAPVRLENHDDPQSLYLLQSGLTG
                     PQPTPTQLSDFTSPRSDYRMGSAQELRVPLSWTNDQGVRVTKTYVFYRGKYEIGIEYN
                     IENHSDAPWTGAPYAQILRNDPRTKRSMFNVESYAFHGPAMWDGTKYRKLDTADKDDS
                     HLAIDVRDGWIAALQHHFVSAIVPPVDVPYHFTLAASGDQYRLAATGPAHTVAPGASA
                     TFGATLFIGPKLQGQLKATSPELARAADYGHLYMLAQPLFWLLDHVHAITGNWGVAII
                     FVTFLLKLLFYPLSEASGRSMAKMKQLGPRIKNLQETYKDDTEKRGRAMMELYKREKV
                     NPVAGCLPTVIQIPVFLAFYWVLLESVEMRQAPFAFWINDLSSRDPYFILPLIMMGAM
                     FVQSRLNPAPADPVQAKVFMFMPLVMSCMFAFFPAGLVLYWVTNTILGIAQQWNINRR
                     IAAAAAARKN"
     gene            2007..3359
                     /gene="mnmE"
                     /locus_tag="VFB37_16430"
     CDS             2007..3359
                     /gene="mnmE"
                     /locus_tag="VFB37_16430"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZO24085.1"
                     /translation="MSRIDTIVAGATPPGRGGIAIVRVSGPKVPEIAAVILGELPPAR
                     RATFARFQDAHHDTIDAGLALFFPSPHSYTGEHVLELQGHGGALVVEALVSRIVEIGG
                     RRALPGEFTQRAFLNDKLDLAQAEAIADLIDAGSRAAVRAAMRSLQGEFSAMVRGLTD
                     AIIDLRTYVEAAIDFPEEEIDFLADEELTTRFQSVGEHFQGVEQSATQGRLLRDGMTI
                     VIAGRPNVGKSSLLNRLAGYDAAIVTPIPGTTRDIVRERIDIDGMPLHVLDTAGLRQG
                     AGLVEAEGIRRATAEMVRADRVLFVMDLVEDPGGAAFREEAARMPADVPVTLIFNKCD
                     LAVGLPVADTITGPPRVTISAATGKGIDKLRDHLKQCMGYQTVDAGTVSARQRHLESL
                     KRASAHVQEAKRQLQENRAGELVAEELRGAQQALGEITGEFTTEDLLGRIFSSFCIGK
                     "
     gene            complement(3412..3981)
                     /locus_tag="VFB37_16435"
     CDS             complement(3412..3981)
                     /locus_tag="VFB37_16435"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HZO24086.1"
                     /translation="MKPTLALATLLALIPLAALQPAFAAETKPSEQAVQQLLEAMHTG
                     DAVKEASAHMDDMLRESMKDAGGHPLNAEQEKIRDETRAKVVALIRKQLDWSTILEPM
                     MVQSYRDTFTPEEVNALLKFYRSPIGKSVAEKLPAASQQTMRLMQTRMREMLPQIQEI
                     VRDSKARMQAAATAPGEPPQTGATPPPPH"
     gene            complement(4035..5459)
                     /locus_tag="VFB37_16440"
     CDS             complement(4035..5459)
                     /locus_tag="VFB37_16440"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZO24087.1"
                     /translation="MSDSGKSQPPPTSTTYMVGGNVTGLSGNGLMLELNGGSDIPVES
                     NGAFTFPGSLSSGTQYNVIIKDQPAVRREICTVTNGAGTVGMADIGNVSINCVVAVGF
                     VYVVDPNSQIGAYGITPGTGAPIPDGSFSIPTASVYQAMVAAPSGNFLYVLGQEPSQI
                     STFAIDPDQGALTPVNGLVDIGPGGATSMVMSPSGSFLFVESTDTLSESVQTFKVDAA
                     TGALTPGVILQRSISCIPGACAGAGRFVVGSDSRYLYRLDLDLANNTGSVTPYAIDPV
                     TGALTAGTAVSFAANAANTDGTSMAIDTLGRFLYVATSAGVPLMDTSATVATYMIDPK
                     SGALSPGSTSSVANGAFLMVPDPTGRYLYSIYWGGDQLISNILSLAVDPSSGALSQIG
                     PAVPIAVTTDVAACDPSGGFLFLGAGGGAPFTGNLGGNDLTSFTIATGGVSPGAVSLS
                     GEGMHIPVESGSTPPGGSALAIVE"
     gene            complement(5748..6536)
                     /locus_tag="VFB37_16445"
     CDS             complement(5748..6536)
                     /locus_tag="VFB37_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO24088.1"
                     /translation="MKAIIVDDEAQARKTLRERCATEQDIEVVGEYADAESALAALLE
                     RAPDVMFLDVQMGRISGMQLAQALEPDSAPLIVFVTGFDRYALRAFELNAADYLLKPF
                     DGQRFRSMLYRLRERHAARNCSRCQVAAATLVELQRLSQQRLEVKPRLLAETDGALHM
                     IDVAEIELVTADRNYVVLTVGKDTFHVRSTLIQAGESMQSQPMLQISRSCLMNINHVR
                     QVSRTARGDYVFVLDGGTTVASSERFRPRVRERIEQFVVRGTRD"
     gene            6659..7327
                     /locus_tag="VFB37_16450"
     CDS             6659..7327
                     /locus_tag="VFB37_16450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO24089.1"
                     /translation="MPEKIQQPRPRPGARENLLEASFVLIREQGYSATSVDELCAKAG
                     VSKGAFFHHFQSKDALALAALDRWSELSMALFQGAPYHKLRDPLDRLLGYLDFRKALV
                     KGEVAEFTCLAGTIVQEAYGTHAELREACDASISGQAATLEADIAEAMKLYGVRAKWS
                     AQSLALYTQAVLQGSFILAKAKDSPDVAVASIDHLRRYIELLFLTGEPSIPPTRRPAH
                     TKEK"
     gene            7331..7807
                     /locus_tag="VFB37_16455"
     CDS             7331..7807
                     /locus_tag="VFB37_16455"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO24090.1"
                     /translation="MRRSAMSRRTLLGAGACTLAAAATLPAIANAGAEPGLSSKNEEL
                     VRKYYKGWEIKDWPAFDRLLTEDFTFTSPMDAHINKSAFKKGCWDTQIPFIGHFDLEH
                     VVGKGEDAFVMYLGHTTNGKTFRNVEYLRLKGDKVEAVECYFGAQNSYTSAVAGHK"
     gene            7974..8357
                     /locus_tag="VFB37_16460"
     CDS             7974..8357
                     /locus_tag="VFB37_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24091.1"
                     /translation="MARIRGGVSLPQTVGAALETVLGEQVRRVRVVEHSLFVRLHGRA
                     VATTRRGRIYLRNSAAEFFEDPWLMLHEYWHVIGQWEPRKLTTGRYLREWLRHGYWNN
                     RFEVEARAFADAHVHELRILLAGAR"
     gene            complement(8387..9565)
                     /locus_tag="VFB37_16465"
     CDS             complement(8387..9565)
                     /locus_tag="VFB37_16465"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO24092.1"
                     /translation="MHAIFSVFRKEFRENLRDRRTVISALIFGPLFGPLLFGATLSMS
                     IEHGTAESDRIVTLAVSHAERAPNLVAYLQQYRVKVEQVSFDDAAARAAVKEHRRPML
                     LLIADDFGTRLSASEPAPLSLYVDSSYAANAKVVARIRNLLSQYGGLLARLRVLARGI
                     DPLVLSPIVVRDIDVSTPASRSVLALGTLSYLVLLTMLMGGMYLAIDATAGERERGSL
                     EPLLTVPVRREHLIYGKMLATCAYMTLSLSLTVTAFAIVLQFAGLERFGMSVNFGAGV
                     ALRIVVLCLPLVPLGAALMTIVAAFTRSYREAQTYLGLVLLVPTLPLVFAGVLGLRPS
                     APLMAIPSLSQHFLITSLLRDESLPASYVALSVGVTLAIAAVLVTIAGRLYRREALLG
                     "
BASE COUNT         1838 a         3048 c         3055 g         1730 t
ORIGIN      
        1 gtcgtgctct cagtacgcga tcgaggcgat ccgatgcttc ggcgtgggac gaggcagctg
       61 gctcaccctg aagcgcctgg gccgatgcca tccgtggcat ccgggcggct tcgatcccgt
      121 gcccacggaa cacggtgcga tgtcctgcag gcgtcctgcg catgactaac aatccgcgac
      181 tcctgctgtg gctggcactg atcgcgatgc tctggctgaa tttcgtcgcc tggaatcgcg
      241 actacggcga gcgcccgcag cccgcgtcca gtcagccggc tggcgccgga tcgagcgcag
      301 ccggtgcccc gagctcggtc gatctcgcca accaggtgcc gcaggcgcag aaggccccat
      361 ccagctccgc caccggccag cccgcacagc cggcctcgcc tcccaccgag agcagcgtgt
      421 cgtcgccgcc tgcgccgacg actgccagca cggagcttgg atcgggagtg gtccacgtcc
      481 agaccgatgt gctcgatatg gatatcagca ctcgcggggg cacgatcgag cgcgtcgatc
      541 tgccgaaata tccgcaggtc aagggtcaag ccgctcccgt gcggctcgag aaccacgacg
      601 atccgcaatc cctttacctg ctgcagtcgg ggctcaccgg gccccagccc accccgaccc
      661 aactgtccga tttcacgagc ccgcgcagcg attaccgcat gggaagtgcg caggagctgc
      721 gggtcccctt gtcctggacc aacgatcaag gcgtccgggt caccaagacg tacgtctttt
      781 accgcggcaa atatgaaatc ggcatcgagt acaacatcga gaatcactcg gatgcgccct
      841 ggaccggtgc tccctacgca cagatcctga gaaacgatcc gcgcaccaag cgctcgatgt
      901 tcaatgtcga gagctatgcc tttcacggtc ccgccatgtg ggacggcacc aagtatcgca
      961 agctggacac ggcggacaag gatgacagcc atctcgcgat cgatgtccgc gatggctgga
     1021 tagcagcgct gcagcaccat tttgtgagtg ccatcgtccc gccggtggac gtgccctacc
     1081 acttcacgtt ggccgcctcc ggggaccaat acaggctcgc ggcgaccggt ccggcccaca
     1141 ccgtggcccc cggcgcctcc gccactttcg gagccacgct gttcatcggg ccgaagctgc
     1201 aggggcagct caaggcgact tcgcccgagc ttgcgcgcgc ggccgactat gggcacctgt
     1261 acatgctcgc ccagccgctg ttctggctgc tcgaccacgt gcacgccatc acgggcaact
     1321 ggggggtcgc gatcatcttc gtgaccttcc tgctgaagct gctgttctac ccgctttccg
     1381 aggccagcgg ccgctcgatg gccaagatga agcagctcgg accgcgcatc aagaatctgc
     1441 aggagaccta caaggacgac accgagaagc gcggccgcgc catgatggag ctgtacaagc
     1501 gggagaaggt gaaccccgtc gccggatgtc tgcccacggt catccagatt ccggtctttc
     1561 tcgccttcta ctgggtgctg ctcgagagcg tcgagatgcg ccaggcgccg tttgcgttct
     1621 ggatcaatga cctttcctcg cgcgatccct acttcattct gccgctcatc atgatgggcg
     1681 ccatgttcgt ccagtccagg ctcaatcccg cacccgccga tcccgtgcag gccaaggtgt
     1741 tcatgttcat gccgctggtg atgtcctgca tgttcgcgtt cttcccggcg ggactggtgc
     1801 tgtactgggt gacgaacacc atcctgggca tcgcgcagca gtggaacatc aatcggcgga
     1861 tcgccgccgc cgcggcggcc agaaaaaact agcccttcgg ggcggactgg agaacccgag
     1921 gccgcttgtc ggccggcacg ggttttcgac cgtgcctcgc gccgctattc ttgagaactt
     1981 cggcacccgg cgaaacaggg ctgcgcgtga gcaggatcga taccatcgtg gcaggggcga
     2041 cgccgcctgg gcgcggcggg atcgccatcg tccgtgtctc gggaccaaaa gtccccgaga
     2101 ttgctgcggt gatcctcggc gagctgccgc cggccaggcg cgcgacattc gcccgattcc
     2161 aggacgccca ccacgacacg atcgatgcgg ggctcgcgct cttctttccc tcgccgcact
     2221 cctataccgg tgagcatgtc ctggagcttc agggtcacgg cggcgcgctg gtggtcgagg
     2281 ccctcgtgtc gcgaatcgtg gagattggcg gtcggcgcgc actgccgggt gagttcacgc
     2341 agcgggcttt tctcaacgac aagctcgacc tggcgcaggc cgaggccatc gcggacctga
     2401 tcgatgcggg ctctcgcgct gccgtgcgcg cagcgatgcg ttcccttcaa ggtgagttct
     2461 ctgcgatggt gcgcggactc accgatgcga tcatcgatct gcgaacctac gtcgaggccg
     2521 cgatcgattt ccccgaggag gagatcgatt tcctcgcgga cgaggagctc accacccgct
     2581 ttcagtcagt cggagagcac tttcagggag tcgagcagag tgcgacgcaa ggacggctgt
     2641 tacgcgatgg tatgacgatc gtcatcgccg ggcggcccaa tgtcggcaaa tccagcctgc
     2701 tgaaccgtct tgccggttat gacgcagcaa tcgtcactcc aatcccgggt acgacgcgcg
     2761 atatcgtgcg cgagcggatc gacatcgacg gaatgccgct gcatgtgttg gatacggccg
     2821 gcctgcgcca gggcgccggc ctggtcgagg ccgagggcat tcgccgagcc acggcagaga
     2881 tggtgcgggc cgatcgcgtg ctgttcgtaa tggacctggt ggaggatccg ggcggcgccg
     2941 cgtttcgaga ggaagctgcg cgcatgccgg ccgatgtgcc ggtcacactg atcttcaaca
     3001 aatgcgatct cgcggtgggg ctgccggtcg cagacaccat cactggcccc ccgcgagtca
     3061 cgatttctgc ggccaccggc aagggaatcg acaaactgcg ggatcacctc aaacaatgca
     3121 tgggctacca gactgtggac gccggcaccg tgtcggcgcg ccagcgacat ctggagtccc
     3181 tgaagcgcgc cagcgcccat gtgcaggaag ctaagcgtca gctccaggag aatcgcgcgg
     3241 gtgagctcgt cgccgaggag ctgcgaggcg cgcaacaggc tctcggcgaa atcaccggcg
     3301 agttcaccac ggaggatctg ctcgggcgga tcttctcgag cttttgtatc ggaaagtgat
     3361 cggcatcgga gctgacctcc gagaaatcgc cgcagcgatt ttgcccatga gctagtgcgg
     3421 cggcggcggc gtcgcgcctg tctgcggcgg ctcccccggt gcagtggcgg cggcctgcat
     3481 gcgcgccttg gagtccctca cgatctcctg gatctgcggc agcatctcgc gcatgcgcgt
     3541 ctgcatgagg cgcatcgtct gctggctggc ggccgggagc ttctcggcca cggacttgcc
     3601 gatcgggctc cggtagaact tgagaagcgc attcacctcc tccggggtga aggtgtcacg
     3661 gtacgactgg accatcatcg gctcgaggat ggtcgaccag tcgagctgct tcctgatgag
     3721 cgcaaccacc ttggcgcgcg tctcgtcacg gatcttctct tgctcggcat tgagtggatg
     3781 accgccggcg tccttcatcg actcgcgcag catgtcatcc atgtgcgctg aggcctcctt
     3841 gaccgcgtcc cccgtgtgca tcgcctcgag cagctgctgc accgcctgct ccgagggctt
     3901 ggtctcagca gcgaacgctg gctgcagcgc ggcgagcggg ataagcgcga gcagcgtcgc
     3961 cagagctaga gtgggtttca tacgagcctc cagccgtatc gtgtcgccat gagccatcat
     4021 ggcgcgatcg tgcgctactc gacaatcgca agcgcgctgc cgccgggcgg agtagatccg
     4081 gactcgaccg ggatatgcat cccctctccg ctcaacgaaa cggctccggg actgacgccg
     4141 ccagtcgcga tcgtgaagga agtcagatcg ttcccgccga gatttccggt gaaaggagct
     4201 cccccgccag cgccgagaaa aaggaagccg cctgaaggat cgcaagccgc cacatcagtc
     4261 gtcacggcga tcggaaccgc gggaccgatc tgcgacaatg cgccgctgga tggatccacg
     4321 gcgagggaga ggatgttgct gattaattga tcaccacccc agtagatgga gtagagatac
     4381 cggccggttg ggtccgggac catcaggaag gcaccatttg ccactgacga ggtcgagcct
     4441 ggcgatagcg ccccgctttt gggatcgatc atgtaggtgg ccacggtcgc agatgtgtcc
     4501 ataaggggca cgcccgctga ggtggccaca tacagaaacc tgcccagcgt atcgattgcc
     4561 atgctcgtgc cgtctgtgtt cgctgcgttg gcggcgaatg atacagcggt gcccgccgtc
     4621 aacgcaccgg tcacaggatc gatcgcgtag ggagtcacgc tgccagtgtt gttggccaga
     4681 tcgagatcca gacggtaaag atacctgctg tccgatccga cgacgaagcg gccagccccc
     4741 gcacaggctc cggggataca gcttattgaa cgctgcaaaa taacgcccgg cgttaaggcc
     4801 ccggtggccg cgtcaacctt gaaggtctgc acgctctccg aaagggtatc ggtgctttcg
     4861 acaaacagga aggaaccgct tggactcatg accatgcttg tagccccacc cggcccgata
     4921 tcaaccagtc cattgaccgg cgtcagtgcg ccttgatcgg ggtcgatcgc gaacgtcgaa
     4981 atctggctcg gttcctgtcc caggacgtag aggaagttgc cgctgggggc cgccaccatc
     5041 gcctggtata cactcgcggt tggaatcgag aaactgccat cagggatcgg cgcacctgta
     5101 ccgggggtga tgccgtaagc gcctatctgg ctgttcggat ccaccacgta aacaaatccc
     5161 acggcgacga cgcagttgat cgagacgttc ccgatatccg ccatcccaac cgtacctgct
     5221 ccatttgtta ctgtgcagat ctcacggcgc accgcgggtt ggtccttgat gatgacgttg
     5281 tattgagtac cgctcgaaag cgagccgggg aatgtgaatg ctccgttgct ttcgacggga
     5341 atgtcggacc ctccattgag ctcgagcatc aggccattgc cggagaggcc cgttacgttg
     5401 ccgccgacca tgtaagtggt gctggtcggt ggcggctggg atttacccga atcactcatg
     5461 ccttgacccg gatcactcat gccgccacaa gagctcagca acaggagtac aggtattacc
     5521 gtgaaggtct ggagtgtgcg agccatgaga ggactccctg aaagcttcca acttccgttt
     5581 tggtgcggcg caactggtac tggaatatgt tatgaaaccg aagcgcagct ctgcgagacc
     5641 ggcagcctga agaccgccta ccaagcctct gcttacacag taagccagcg cgttgctccc
     5701 cgcaacttcg gggaatgctc cctgtcctcc cggaatggca gccgctgtca gtccctcgtt
     5761 ccccgaacca cgaactgctc gatacgctcg cggaccctgg gacgaaatcg ttcgctgctc
     5821 gccaccgtcg ttccgccatc gagtacgaac acgtaatcgc cgcgcgcggt acggcttacc
     5881 tggcgaacat ggttgatgtt catgaggcag gagcggctga tctgcagcat cggctgtgat
     5941 tgcatggact ctccggcctg aatgagcgtg ctgcgcacgt ggaaggtgtc cttgcccacc
     6001 gtcagcacga cgtagttccg atcggcagtc acgagctcga tctcggcgac atcgatcatg
     6061 tgcagcgcgc catcggtctc ggcgagcagg cgcggcttga cctcgaggcg ctgctgcgac
     6121 agccgctgca actcgaccag cgttgccgcc gcaacctggc agcgcgagca gttgcgtgcc
     6181 gcatggcgct cacgcaagcg atacaacata ctgcggaagc gctgcccgtc gaaaggcttg
     6241 aggagatagt ccgcggcgtt caactcgaag gcccgcagcg cgtaccggtc gaacccggtc
     6301 acgaagacga tcagtggcgc ggaatccggc tcgagcgcct gggcgagctg cattcctgaa
     6361 atccgaccca tctgcacgtc aaggaacatc acgtcaggtg cccgttctaa cagggcggcg
     6421 agcgcactct cggcgtccgc atattctccg accacctcga tgtcctgctc ggtggcgcaa
     6481 cgctcgcgca aggtcttcct ggcctgcgct tcatcgtcga cgatgatcgc tttcattggc
     6541 gtctctcgac actcaagaac tcgagcgcgc gggcaggtgg atacgcaacc acggtctgtc
     6601 ggaaagtcct cggtcgggtg tattgacgct caaaccgact agtcggtatg ataccgcggt
     6661 gccagaaaaa atccagcagc cgcgcccgag gccaggggct cgcgagaatc tgcttgaagc
     6721 atctttcgtg ctcatccgcg agcaaggcta ctcggccacc tcggtcgacg agctctgcgc
     6781 gaaggcagga gtgagcaagg gtgcattctt ccatcacttc cagagcaaag atgcgctcgc
     6841 cttggccgcg ctggatcgct ggtccgagct ctcgatggcc ttgtttcagg gggcgcccta
     6901 ccacaagctc cgcgatccgc tggatcggct tctgggctac ctcgacttcc gcaaggctct
     6961 tgtgaagggg gaagtggcgg agttcacctg tctcgccggc acgatcgtgc aagaggctta
     7021 tggcacccat gccgagttgc gggaagcgtg cgatgcgtcg atcagcggac aggcggcaac
     7081 gctggaagcc gacattgccg aggcaatgaa gctttatggc gtgcgtgcaa aatggtcggc
     7141 gcagagcctg gcgctctaca cccaagcggt cctgcagggt tccttcatcc tcgcaaaggc
     7201 caaggacagt cccgacgtcg cggtggccag catcgatcat ctgcgccgct atattgaatt
     7261 gttgttcttg accggcgagc cttcgatacc acccacccga aggccggccc atacaaagga
     7321 gaagtgagtc atgagaaggt cagcgatgtc gcgccgaaca ttactggggg ctggagcgtg
     7381 cacactggcg gcagccgcca cccttccggc gattgccaat gcgggcgcag agccaggact
     7441 gagctcgaag aatgaagagc tcgtccggaa atactacaaa ggctgggaga tcaaggactg
     7501 gccggccttt gacaggctgc tgaccgagga cttcactttc acgagcccga tggacgctca
     7561 catcaacaag agcgccttca agaaaggatg ctgggatacc cagatcccct tcatcgggca
     7621 ctttgacctg gagcacgtgg tgggaaaggg cgaagatgcg tttgtgatgt atctcggtca
     7681 caccacaaac ggaaagacgt tccggaacgt ggagtatttg cggctcaaag gcgacaaagt
     7741 ggaggccgtc gagtgttact tcggcgcgca gaacagctat acatctgccg tggccgggca
     7801 taaatgacag cctgagcgct tcgctttgcc gtgattgctc ggggggatgg ggaaatgggg
     7861 gctgttggca ggtctctggc tatcctgatc acgctcgcca cggttgcttg caacacgatc
     7921 atcaactcga ccgcacacag gtcacgcgca gaaaatcggg tatcctgcgg cgcatggccc
     7981 ggatcagagg cggggtttcc cttccgcaga ctgttggtgc ggcgctcgag accgtgctgg
     8041 gcgagcaggt ccgccgggtg cgcgtggtcg agcattccct gttcgtgcgg ctgcatgggc
     8101 gcgcggtcgc aacgacccgg cgcggccgca tctatctgcg caacagcgcg gcggagttct
     8161 tcgaggatcc gtggctcatg ctgcacgagt actggcacgt gatcggccaa tgggaaccgc
     8221 gcaagctcac caccggccgc tatctgaggg aatggctccg tcacggctac tggaacaatc
     8281 gcttcgaggt cgaggcgcgc gcatttgcgg acgcgcacgt gcacgagcta cggatcctgc
     8341 tcgcgggtgc acgctgacag cggtacctgc cgaaacccgg cgggtctcac cccagcagag
     8401 cttcacgacg atagagccgg ccggcgatgg tcacgagcac cgcggcgatt gcgagtgtga
     8461 cacctactga gagggcgacg tacgaggccg gcagagactc atcacgcagc aggctcgtga
     8521 tcagaaagtg ttggctgagc gaaggtatcg ccatcagggg cgctgagggc cgcaaaccca
     8581 gaaccccggc gaagacgagc ggcagggtgg gcaccagcaa taccaggccc agataggtct
     8641 gcgcttcccg gtagctgcgg gtgaaggcgg ccacgatggt catgagagcg gctccgagcg
     8701 gcacgagtgg tagacacagc accacgattc gtaacgcaac gcccgccccg aaattcacac
     8761 tcatcccgaa ccgctcgagc cctgcaaact gcagcacgat cgcaaatgcg gtgaccgtga
     8821 gggacagcga caaggtcatg taggcgcagg tcgcgagcat cttgccgtag atcagatgct
     8881 cccggcgcac gggcaccgtg agcagtggct cgagcgagcc acgctcgcgc tcgcccgccg
     8941 tggcatcgat cgccaggtac atcccgccca tcaacatcgt caggaggaca agataactga
     9001 gcgttccaag ggcgagcacc gagcggctcg caggcgtgga gacatcgatg tcccgcacga
     9061 ctatgggtga gaggacaagc gggtcgatcc cgcgggccag cacccgcagg cgcgccagca
     9121 ggcccccgta ctggctcagc aggttacgga tgcgggccac caccttggca ttcgcggcgt
     9181 aggaggaatc cacatacagg ctcagcggcg ccggctcgga tgcgctcagc cgggtgccga
     9241 agtcatcggc aatgagcagc agcatgggcc ttcgatgctc cttcacggcc gctcgcgcag
     9301 cagcgtcatc gaagctgacc tgctccactt tgacccggta ctgctgcaga taggcgacca
     9361 gattgggcgc ccgctcggca tgcgagacgg cgagcgtcac gatgcggtcc gattcggccg
     9421 tgccatgctc gatactcatg gagagcgtgg cgccgaacag cagcgggccg aacagcggcc
     9481 cgaagatcaa cgcggagatc accgtccggc ggtcgcgcag gttctcgcgg aactccttgc
     9541 ggaacacgct gaagatggca tgcatcaggc gagtccttcg ctcgtgccga tgagatgcac
     9601 gaaggcatcc tcgagctcgt tctggcctgt ttgcgcgcgc agtgcatcag gcgtgccgct
     9661 ggctaccacg g
//
LOCUS       DASRHN010000406         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_972499, whole genome shotgun sequence.
ACCESSION   DASRHN010000406 DASRHN010000000
VERSION     DASRHN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972499"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VFB37_16470"
     CDS             <1..1229
                     /locus_tag="VFB37_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZO24093.1"
                     /translation="GAAAAAAAVASTAKAVAAGAPPPALSSITTSAQPITAQEREGRI
                     GKVQSLLQQQRIAAFLVESGSSLEYFTGVRWHRSERVTAAIIPASGRTVVVTPFFEQP
                     SVRETLQIDSDVRPWNEDENPFELIAAAARGDAKAGSSLAIEPTTRYYIIDGVSKASG
                     PPNLISGDQLVRACRMVKSPAELALMQAANNVTIAALREVHGRVQAGMQPSDLASMMD
                     EATRQLGGSSVEFSLVLLNEASAYPHGSHQSQTVHPGSVILMDCGCTVHGYQSDISRT
                     WVFGEPTKRQREVWDTVKRGQELALETAALGKPVGSIDKAVRDFYEQRGWRKNYGLPG
                     LSHRTGHGIGMDGHEAPYLVRNDTTPLAAGMCFSDEPGIYIPGEFGIRLEDCWYMTSN
                     GPKLFTALAKSIDQPI"
     gene            1279..2172
                     /locus_tag="VFB37_16475"
     CDS             1279..2172
                     /locus_tag="VFB37_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZO24094.1"
                     /translation="MSWRSWLAFAALGVIWGVPYFFIKLAVQEVSPFIVAWGRITLAA
                     AILMPIAWRRGALHSLAAHKGVVLAFAIAEFVIPFSAISIGERWIDSSVTGILIAVVP
                     LTITLISRFFGVHERLGTARLSGLGIGLLGVVALLGLGSISGVLGWIGVGCMLLATLG
                     YAIGPLIIQRHLRELDSIGPIAASLLLSSLILLWPALATFPHQMPSPLALWSIGVLGV
                     ACTALAMLLMFYLVGHAGASRASVITYINPAVASLLGVLVLHERLGVGGILAFGLILL
                     GCWLATRGKAGHAEAGSPASA"
     gene            complement(2376..3110)
                     /locus_tag="VFB37_16480"
     CDS             complement(2376..3110)
                     /locus_tag="VFB37_16480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO24095.1"
                     /translation="MKHALVTGASGGIGRAIALALAERGFSIVLHYRKDQKAAEETRR
                     RLPGDGHLIVQADLEDPAATERLWREACAERRVDTLVNNAGIFPNHPPLSTDFGGWTA
                     AWQRTLATNLTAVANLSYLASQTMVKEGGGRIVSISSRGAFRGEPNAPAYAASKAGLN
                     AFSQSMAKALAAAHVYVYVVAPGWVATDRVAAAIKDQAVLADQPLGRVATPEEVARVV
                     TFCALDAPGSMTGAILDVNGASYLRT"
     gene            complement(3336..3737)
                     /locus_tag="VFB37_16485"
                     /pseudo
     CDS             complement(3336..3737)
                     /locus_tag="VFB37_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
BASE COUNT          591 a         1250 c         1187 g          709 t
ORIGIN      
        1 tcggcgctgc cgctgccgcc gccgccgtcg cctcgaccgc gaaggctgtc gcagccgggg
       61 cgccgccgcc cgctctgagc tccattacca cttctgccca acccatcacg gctcaggagc
      121 gggagggccg catcggcaag gtgcagtccc tcctgcagca gcagcggatc gcggcctttc
      181 tggtcgaatc aggctccagt ctggagtatt tcaccggcgt gcgctggcac cgctccgagc
      241 gagtcaccgc agcgatcatc ccggcgagcg ggcgcacggt cgtcgtcact cctttcttcg
      301 aacagccctc cgtccgggag accttgcaga tcgacagcga cgttcgcccg tggaacgagg
      361 atgaaaaccc gttcgagctc attgccgcag ccgcgcgcgg cgatgctaag gcgggcagtt
      421 cccttgcgat cgagcccacc actcggtact acatcatcga cggcgtcagc aaggcatcgg
      481 gtccgcccaa cctcatttcc ggcgatcagc tcgtgcgtgc ctgccggatg gtgaaatcgc
      541 cggcagagct ggccctgatg caggccgcca acaacgtcac gatcgcagcg cttcgcgagg
      601 tgcacggccg ggttcaggcc ggcatgcagc cttcggacct ggcaagcatg atggatgagg
      661 cgacgcggca gcttggcggc agcagtgtgg aattctcgct ggtgctgctc aacgaggcga
      721 gcgcctatcc gcatggatcg caccagtcgc agaccgtgca tcctggctcg gtgatcctca
      781 tggactgcgg ctgcaccgtt cacggctatc aatcggacat ttccaggacc tgggttttcg
      841 gcgagcccac caagcgccag agggaggtat gggacaccgt aaagcgcgga caggagctcg
      901 ctctggagac cgccgctctg ggcaagcccg tgggctccat cgacaaggcc gtgcgcgact
      961 tctatgagca gcgcggctgg cgcaagaact acggcttgcc gggattgtcg catcgaaccg
     1021 gacacgggat cggcatggat ggccatgagg ccccgtacct ggtccgcaat gacacgacgc
     1081 cgctggcagc cgggatgtgc ttttccgacg agcctgggat ctacattcca ggtgagttcg
     1141 gcatccgtct cgaggactgc tggtacatga cctccaacgg cccgaagctg ttcaccgcgc
     1201 tggccaagtc gatcgatcag cccatctgag caccatgacc gaggtggcct cgagctgctt
     1261 gcaacaggat cgacaggcgt gagctggcga tcttggctgg ctttcgccgc gctgggcgtg
     1321 atctggggcg tgccctactt cttcatcaag ctggccgtgc aggaggtctc gccattcatc
     1381 gtggcgtggg gccgcatcac tctggctgct gcgatcctca tgccgattgc ctggcgccgg
     1441 ggcgctctgc attcgctcgc ggcccacaag ggcgtggtgc ttgcgttcgc gatcgccgag
     1501 ttcgtgattc ctttttcagc catctccatc ggcgagcggt ggatcgactc ctccgtgacc
     1561 gggatcctga tcgcagtggt gcccttgacg attaccctga tttcgcgctt cttcggggtg
     1621 catgagcgcc tgggaactgc gcgcctgagc ggtctcggca tcgggctgct cggagtggtg
     1681 gcgctacttg ggttgggaag catctccggc gtgttgggtt ggatcggtgt cgggtgcatg
     1741 ctgctcgcca ctctggggta tgcgatcggt ccgctcatca tccagcgtca cctgcgcgag
     1801 ctcgactcca tcgggcccat cgcggccagc ctgctgctct cgagcctgat cctgctgtgg
     1861 cccgcgctag cgacctttcc gcatcagatg ccctctccac tcgcgctgtg gtccatcggc
     1921 gtactcggag tcgcgtgcac cgcgcttgcc atgcttctga tgttctacct ggtaggccat
     1981 gccggcgcct cgcgcgcatc cgtcatcacg tacatcaatc cggcagtggc gtccctgctc
     2041 ggcgttctgg tgctgcacga acgcctcggg gtcggcggaa ttctggcctt cgggctcatt
     2101 ctgctgggct gctggctcgc aacccgcggc aaggccgggc atgccgaggc cggctcccct
     2161 gcctccgcct gatggaggcc tccccgcatc gggcggcccc gcgtcggacg gctcttacat
     2221 caggacgggc ggattggtga ttggcgccac ctgggagcag gtgcacactg cagccgtaga
     2281 ttagagatag tcctcgcgac gaagatcagt ctacttcgag ctcaaagggc cgcaggaagc
     2341 ggccctttgc ctatgcgggc gctccgatcg ggcgctcagg tgcgcagata cgaggcccca
     2401 ttcacatcga gtatcgctcc ggtcatgctg ccaggggcat ccagcgcgca gaaagtcacg
     2461 acccgggcca cttcctcggg agtcgccacg cggcccagcg gctgatcggc gagcacggcc
     2521 tggtccttga tcgccgccgc cacccgatcg gtggccaccc agcccggggc caccacgtag
     2581 acatacacat gagcagccgc cagcgccttc gccatcgact ggctgaatgc gttgagcccc
     2641 gccttgctgg ccgcgtaagc aggcgcattg ggctcgcccc tgaaagcgcc ccgcgaagag
     2701 atgctcacga tgcgtccccc accctccttc accatggtct gtgaagccag gtacgacagg
     2761 ttggccacag cggtcaggtt ggttgcgagc gtccgctgcc acgctgcggt ccaaccgcca
     2821 aaatccgtgc tcaggggcgg atgattgggg aagataccgg cattgttcac gagcgtatcg
     2881 acacggcgct ctgcacaggc ctctcgccac aggcgttccg tcgccgcggg atcctcgaga
     2941 tcggcctgca cgatgaggtg gccgtctccc ggcaggcgcc gccgggtctc ctccgccgcc
     3001 ttttggtcct ttcggtagtg caggacgatg ctgaagccgc gctcggcgag cgccagcgcg
     3061 atggctcgtc cgattccgcc ggaagcgccg gtgaccagag cgtgtttcat cgagcaagac
     3121 ctgcaggtga agggagcctg accgcggcac tatcccgcgt gttcacactg cattctactg
     3181 cggctgcggg cgccgcagtc gcccctcctg aactaatgcg ccggcacggg ctgcttgatg
     3241 ttgcgacgtc cgggacggcg cgtgcgcttg tcctccggca aatccgaggt cgccggtgca
     3301 ggcggttccg gatccatcat gggtcgctgc tgcgtcaggg caagtgtcag gacctcgtcg
     3361 atccacttga ccggcttgat ctcgagattg cccttgatgt tgtccggaat ctcgaccagg
     3421 tccttctgat tctcatccgg aatcaacacc gtcttgatac cgccgcgatg cgcggcgagc
     3481 agcttttcct tcaggccgcc gatgggcaac acctcgccgc gcagggtgat ctcaccggtc
     3541 atcgcgacat cggagcgcac gggaaccttg gacaaagccg acaccagtgc cgtgcacatg
     3601 ccgatgcccg cactcggacc atccttcggg gtcgccccct cgggaatatg caggtgcaca
     3661 tcgagtttct ggtagaactc tggctccagg cccaggacct gcgccctgct gcgcacgaca
     3721 gtcatcgcgg cctggat
//
LOCUS       DASRHN010000407         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_314648, whole genome shotgun sequence.
ACCESSION   DASRHN010000407 DASRHN010000000
VERSION     DASRHN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314648"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFB37_16490"
     CDS             complement(<1..816)
                     /locus_tag="VFB37_16490"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HZO24096.1"
                     /translation="MSTQPRPIQRTLMSMLLLTSIVVLLVSTVTSLTYEFLTYRHSAV
                     RALQTLGEVIADNSTAALAFENADDARATLAALKAEPHIIGAVLYDRQGALFAAYPQN
                     VQPEPLSGAPEPPAGYRFEHDHLVGLQPVVQNGRRLGTLYLKSDTRAIFDELRLHGLI
                     AALVIAASCLLAYFMSRSLQRRVSQPILSLAEIAQAVSERRDYSVRAAPAEGYELGKL
                     TEAFNHMLVEIQQQHVKLNVQLGNLQLLQQITRAIGERQDLASLFGIVLRSLEE"
     gene            complement(820..1356)
                     /locus_tag="VFB37_16495"
     CDS             complement(820..1356)
                     /locus_tag="VFB37_16495"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HZO24097.1"
                     /translation="MSLLRAAWLACLAAAMPVLGAAPGEYQVKAVFLFNFSQFVEWPA
                     QAFSAADAPFTLCVLGEDRFGSQLDAAVRGESVQGHPLIVKRYREASELGTCHMLFIG
                     DSETPQLEQILGSLDGNATLTVSDIEHSAERGTIIQFANENNRLRLRINVAAARSAGL
                     TISSKLLRPAEIVGPGRG"
     gene            complement(1344..3326)
                     /locus_tag="VFB37_16500"
     CDS             complement(1344..3326)
                     /locus_tag="VFB37_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO24098.1"
                     /translation="MGGVRRLRPVWALAGILAVSVAHADDAPVSTADLKKLSVEELMG
                     LEVTSVSRRPEKLSEVASAIQVITAQDIEQSGAQSIPEALRLADNLQVAQKNSHDWAI
                     TARGFNTDLANKLLVLVDGRSVYTPLFSGVFWDVQDYPLEDLDRIEVISGPGGTLWGA
                     NAVNGVINVITKSAQDTQGLYLDGGGGSHWEESFTARYGATLAPDVYLRVYGRYFDSN
                     SEVFANGEHAPDSWRQARAGFRLDAQPSTQDGLTVQGDFYNGSADQTTPNAAEFSGGN
                     LLGRWSRALAADSDMSLQAYVDRTHRAQGESPLVLAPAGTIKDDLDTYDVDFQYHFSA
                     AQRHQIVWGLGYRFTHDVVSNAPSLTFLPSVLNQSLYSGFLQDEIQLRPDLFFTAGTK
                     LEHNAYTGFEVEPNVRLHWNFTASQTLWSAISRAVRTPSRIDHDLAEPAPTTGLVVLE
                     GNPDFRSESVIAYEIGYRAQLNSALSTSVSTFYNSYGNIRSTAPSPNPTLPGLPFPLI
                     FRNDVEGHTYGMELSASYRVLEWWRLHAGYTLLQESLHVKPGAVDFNDAHNETADPQQ
                     QASLRFEWSLGSRMDLNAALRWVDSLPINNGPTVENVPSYLELNARLGWRPSERLELS
                     ISGENLLHAHHVEYGFPDPTRIEIERSVFGRVQCHF"
     gene            complement(3456..>4798)
                     /locus_tag="VFB37_16505"
     CDS             complement(3456..>4798)
                     /locus_tag="VFB37_16505"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZO24099.1"
                     /translation="QITYIIDHAQDSVLFMEAAFLPLLAPLRTKLASLRQIILLSDSA
                     DAAALLPEALFYEPWLAQGGGERFEWPAVAEETAATLCYTSGTTGHPKGVVYSHRSNV
                     LHALAINAADGFGLRTRDVMMPIVPMFHANAWGTIFAAPMAGAKLVLPGCRLDGAALW
                     QLLEEERVTVTAAVPTVWLGLLKYLDETGRNLPHLQRVLIGGAAVPRMMLERFERDFG
                     VEVIHAWGMTEMSPVGTFGTLKSADREKDEEVYIRRKLKQGRIPYTVELKVVDAAGAE
                     LPRDGQARGELKARGPAIVSRYFGEADAATDAQGWFATGDVATIDAEGYVQIVDRAKD
                     VIKSGGEWISSIELENTAMGHPQVAEAAVIAVPDEKWGERPLLLVLPKSGERLDPQSV
                     LAHLQGRVAKWWIPERVQIVAEIPHSGTGKIRKDLLRERFGRPVGVSAPTVSKP"
BASE COUNT          868 a         1578 c         1499 g          853 t
ORIGIN      
        1 ctcctccagg ctgcgcagca cgatgccgaa caggctggcc agatcctgcc gctcaccgat
       61 cgcccgcgtg atctgctgca ggagctgcag gttgccgagc tggacattga gcttcacatg
      121 ctgctgctgg atctccacca gcatgtgatt gaaggcttcg gtcagcttgc cgagctcata
      181 gccttcggct ggcgcggcac gcacggagta gtcgcgtcgc tcggacaccg cttgcgcgat
      241 ctcggcgagc gacagaatcg gctgcgagac ccggcgctgc aggctgcgcg acatgaagta
      301 ggcgagcagg catgaagcgg caatcacgag ggccgcgatg agcccgtgca ggcgcagctc
      361 gtcaaatatc gctcgcgtgt cggacttgag atacagcgtc cccaggcgcc ggccgttctg
      421 caccacgggc tgcaaaccca ccaggtgatc atgctcgaag cggtatccgg ccggcggctc
      481 cggggcgccg ctgagcggct ccggctgcac attctgtggg taggctgcaa agagcgcgcc
      541 ctgccggtcg tagagcacag cgccgatgat gtgcggttcg gctttgagtg ccgcaagcgt
      601 cgcccgcgca tcatctgcat tctcgaaggc cagggctgcg gtgctgttgt cggcaatcac
      661 ttcgccgaga gtctgcagcg cgcgcaccgc cgagtgccgg taagtgagga actcgtaggt
      721 cagtgaggtc accgtgctga ccagcagcac cacgatgctg gtcagaagga gcatggacat
      781 cagcgtccgt tggatcggtc gcggctgggt gctcatgcgc taacctcgtc ccgggccgac
      841 gatctcggcc gggcgcagca gcttggagct gatcgtgagc ccggcagagc gggcggcagc
      901 gacgttgatc cgcagccgca gccggttgtt ctcgttcgcg aactggatga tcgtgccccg
      961 ctcggccgaa tgctcgatat cgctcacggt gagcgtcgcg ttgccatcca gactcccgag
     1021 aatctgctcc agctgcggag tctcggaatc accgatgaac agcatgtggc aagtgcccag
     1081 ctcgctcgct tcgcgatagc gcttcacgat gaggggatgg ccctgcacgc tctcgccgcg
     1141 caccgccgca tcgagctgcg agccgaagcg atcctcaccg agcacgcaca gcgtgaaggg
     1201 cgcatccgcg gcgctgaacg cctgtgcggg ccactcgacg aactgggaga aattgaacag
     1261 gaagacggct ttgacctgat actcgcccgg agcggcgcca agcaccggca tggcggccgc
     1321 gaggcacgcg agccacgctg ctctcaaaag tgacattgca cgcgaccgaa gacgctgcgc
     1381 tcgatctcga tgcgcgtcgg gtccggaaat ccgtattcga catgatgggc gtgaagaagg
     1441 ttctcgcccg atatggacag ctccaggcgc tcactcggcc gccatccgag ccgggcattg
     1501 agctccaggt agctgggtac gttctcgacc gtgggtccgt tgttgatggg cagcgagtcc
     1561 acccaacgca gcgccgcatt caaatccatc cgggagccca ggctccactc gaagcgcagc
     1621 gaggcttgct gctgcgggtc ggcggtctcg ttgtgggcat cgttgaaatc gactgcgccg
     1681 ggcttcacgt gcaggctctc ctgcaaaaga gtatagcccg catgcagccg ccaccattcg
     1741 agcacccgat agctcgcgct cagctccatg ccgtatgtat gaccttccac gtcgttgcgg
     1801 aagatcagag gaaacggcaa tcccggcagc gttggattcg ggctgggcgc ggtgctgcgg
     1861 atgttcccgt aactgttata gaaggtcgag acggaggtgc tcagggccga attgagttgc
     1921 gcgcggtaac cgatctcgta ggcgatgacg ctctcggagc ggaaatccgg attgccctcc
     1981 agcaccacga ggcctgtggt tggtgccggc tcggcgagat cgtgatcgat ccgggaaggc
     2041 gtgcgcacgg cgcgcgagat cgccgaccac aacgtctggc tcgcggtgaa gttccagtgc
     2101 agacgcacgt tgggctcgac ctcgaagccg gtgtaagcgt tgtgctcgag cttcgtgccc
     2161 gcagtgaaga acagatccgg ccgcagctgg atctcatcct gcaggaaccc gctgtacagg
     2221 ctctgattca acacggatgg gagaaaggtg agcgagggtg cattgcttac cacgtcatgc
     2281 gtgaagcgat aacccaggcc ccacacgatc tgatgtcgct gcgcggcact gaagtggtac
     2341 tggaaatcga catcgtaggt gtccagatcg tccttgatgg tcccggcagg agcgagcacc
     2401 aggggagact cgccctgcgc ccgatgcgtc cggtccacgt acgcctgcag gctcatgtcg
     2461 gagtcggccg ccagcgcgcg cgaccagcgt cccagcagat tacccccgct gaattccgcc
     2521 gcgttcggcg tggtctgatc cgcgctgccg ttgtagaagt cgccctgcac ggtgagccca
     2581 tcctgcgtgc tcggctgcgc atcgaggcga aagcccgctc tggcctggcg ccaggaatcg
     2641 ggtgcgtgct cgccgttcgc aaagacttcc gagttgctgt cgaagtagcg cccatacacg
     2701 cgcagataca catccggggc gagcgtggcg ccgtagcgtg ccgtgaagga ctcctcccag
     2761 tgcgagccac ccccaccatc gaggtacaga ccctgcgtgt cctgggcact cttggtgatc
     2821 acgttgatca ccccgttcac ggcgttcgcg ccccacagcg tgcctccggg gccgctgatg
     2881 acctcgatgc gatccagatc ctcgagcgga tagtcctgca cgtcccagaa gacgcccgaa
     2941 aacagcggag tgtagacgct gcgcccgtca accagtacga gcagcttgtt cgccagatcc
     3001 gtgttgaatc cgcgcgcggt gatggcccag tcgtgcgaat ttttctgcgc cacctgcaga
     3061 ttgtcggcca gccgcaaggc ctcggggatg ctctgggcgc cggactgctc gatgtcctgt
     3121 gcggtgatga cctgtatggc cgatgcgacc tcggacagct tctcgggccg ccgggatacg
     3181 gaggtgacct cgagacccat caactcctcg acgctgagct tcttcagatc ggccgtcgag
     3241 acgggtgcgt catcggcatg cgccacagaa acggccagta tgcctgccag tgcccatacg
     3301 gggcgaagtc gccgtacgcc acccacgcaa ccctctccct gtgttcgccc agcgcctcac
     3361 gcgccgcgca ttctggattt accttaaccc gtgcgggtcg agcttattcg caagcagcct
     3421 cccggcgacg tgaaccgagc cacagtaccg cgcgctcacg gcttcgaaac ggtgggggct
     3481 gacacaccaa cggggcggcc aaagcgctcc cgcaacagat ccttgcggat ctttccggtg
     3541 ccgctatggg gaatctccgc gacgatctgc acccgctcgg gtatccacca cttcgcgacg
     3601 cgtccctgca gatgcgccag cacgctctgt ggatcgaggc gctcgcccga tttcggcagc
     3661 accagcaaca ggggcctctc gccccacttc tcatcgggca cggcgatgac cgcagcctcg
     3721 gccacctgtg ggtgacccat cgcagtattc tccagctcga tggagctgat ccactcccca
     3781 ccggacttga tcacgtcctt ggcccggtca acgatctgaa cgtagccctc cgcatcgatc
     3841 gtcgcgacat ctccggtggc aaaccaaccc tgagcatccg ttgcggcatc agcctcgccg
     3901 aaataccggg agacaatcgc cggaccgcgt gccttcagct cgccgcgcgc ctggccatcg
     3961 cgaggcagct cggcgccggc ggcatcgacg actttcagct cgaccgtgta cggaatgcgc
     4021 ccctgcttca gcttgcggcg gatatacact tcttcatctt tctcgcgatc cgcactcttg
     4081 agcgttccga aagtgccgac cgggctcatc tcggtcatgc cccaggcgtg aatcacctcg
     4141 acgccaaagt cgcgctcgaa gcgctcgagc atcatgcgcg gcaccgccgc acccccgatg
     4201 agcactcgct gcaggtgtgg cagattcctg ccggtctcgt cgaggtactt gagcaggccg
     4261 agccatacgg tcgggactgc cgcagtcacc gtcacccgct cctcttccag aagctgccag
     4321 agcgccgcgc catcgagccg gcatcccggc aacacgagct tggcccctgc catcggcgcg
     4381 gcgaagatcg tgccccacgc attcgcgtga aacattggca cgatcggcat catgacatcg
     4441 cgggtgcgta acccgaatcc gtcggccgca ttgatggcca gagcatgcaa gacgttggat
     4501 cgatgactgt acacaactcc tttaggatgg cccgtggttc cggaggtgta gcagagcgtc
     4561 gcggctgttt cctccgccac cgccggccac tcgaagcgct cccccccacc ctgcgcaagc
     4621 catggctcgt aaaagagcgc ctccggcaaa agagcggccg catcggccga gtcgctcaac
     4681 aggatgatct gccgaagact ggccagcttc gtgcgcagcg gtgccagcag cggtagaaag
     4741 gccgcctcca tgaagagcac gctgtcttgc gcatggtcga tgatgtaggt gatctgct
//
LOCUS       DASRHN010000408         9521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_148501, whole genome shotgun sequence.
ACCESSION   DASRHN010000408 DASRHN010000000
VERSION     DASRHN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148501"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFB37_16510"
     CDS             complement(<1..822)
                     /locus_tag="VFB37_16510"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZO24100.1"
                     /translation="MWARIWTFLDWCFFGPASAGPGVPARLVRGLRYPYAVVRDLWRG
                     AINLRAMSLVYTTLLSLIPLLAFSFAILKVFGARRDLEPIVYEFFRPVGGQAAQELTE
                     HVLQFAHRVSSGIVGSVGFALLAWTLLGTIKKVEDSFNFLWRVEQPRSFARRIAEYLT
                     LLIVGPVLLVAFVGLSHAAIASVPLQTMAKLPLLQLLHGIALALAPYVMVTAFFTGMY
                     MFIPNTRVRWRPALVGAAAGGVLWAAVGKMFTAFVVYSTRLTVVYAGFAFIVTALL"
     gene            853..1770
                     /locus_tag="VFB37_16515"
     CDS             853..1770
                     /locus_tag="VFB37_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZO24101.1"
                     /translation="MPAQSSTLAGAVAADRPELLGYSPRGMSAQFPQRIVCLTEETTE
                     TLYLLGEDWRIVGISGFTVRPPRARREKPRVSAFTSAKIDRIVALHPDLVLGFSDLQA
                     DIAAQLVRAGIEVHVFNHRSVEEILRMIRTLGGMIGQQEKTRALVERLAGGLQEVRER
                     SAGLEHRPRVYFEEWDAPQISGIRWVSELIAVAGGIDCFPELASQSLARDRIVQDPLE
                     VARRAPDIILGSWCGKRFRPDQVAARPGWDRIPAVRNGYVREIKSPLILQPGPAALTD
                     GVRAIQRIIEEWESGGPALMSQAGLHGDA"
     gene            complement(1772..2572)
                     /gene="djlA"
                     /locus_tag="VFB37_16520"
     CDS             complement(1772..2572)
                     /gene="djlA"
                     /locus_tag="VFB37_16520"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HZO24102.1"
                     /translation="MKFTGKVVGGAIGMLALGPIGAALGVLLGHQFDEYSSAPGNDAG
                     LQHDADASAIGDQFFRAAFRVMGHVAKADGRVSEQEIAAARAVMTDLRLTPAQVQSAI
                     EYFTAGKQPEFNLAAELAALAATCRGRPDLLRVFMEIQVRAALAGSNLEGPSRVLLGR
                     MASRLGVSMVELAQIEAVLRIRSGSFRQQPARQNSAIQLQQAYSVLECAPQASDEEVV
                     KAYRRQLSRHHPDKLKANGLPESMIEHAKQRTQQIIEAYEVIRERRGL"
     gene            2620..3525
                     /locus_tag="VFB37_16525"
     CDS             2620..3525
                     /locus_tag="VFB37_16525"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZO24103.1"
                     /translation="MPSPTVYRTALRGLEKIHEGKVRDIYAVDTQTMLIVTTDRLSAF
                     DVVLPDPIPDKGRVLNEISSFWFSRTADIVPNHLTDRPLESVITDANELALLAGRAVL
                     VRRLAPLPIEAVVRGYLIGSGWKDYQKSRSVSGIELPAGLRQAERLPEPLFTPATKAA
                     PGEHDENIAFAQVEQMVGAAVAKRVRQVALQLYNFAVEHARKRGIIIADTKFEFALDS
                     AGNLTLIDEALTPDSSRFWPADTYRVGASPPSFDKQFVRDYLETLDWNKKAPGPALPE
                     QIIARTSEKYREALKRLTAPVPTAT"
     gene            3552..4232
                     /gene="rpe"
                     /locus_tag="VFB37_16530"
     CDS             3552..4232
                     /gene="rpe"
                     /locus_tag="VFB37_16530"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZO24104.1"
                     /translation="MQTPWIAPSILSADFARLGEEVTAVLRAGADVVHFDVMDNHYVP
                     NLTIGPLVCEALRQYGIEAPIDVHLMIRPVDRIVPDFAAAGATFISFHPEATDHVDRT
                     IELIREHGCKPGLVFNPATPLDYLDYTLEKLDMVLIMSVNPGFGGQKFIPAALQKLAE
                     TRRRIDASGRAVRLEIDGGVKVDNIGAIARAGADTFVAGSAIFESDDYAKTIRAMHSH
                     IEEARRPG"
     gene            complement(4356..4991)
                     /gene="crp"
                     /locus_tag="VFB37_16535"
                     /pseudo
     CDS             complement(4356..4991)
                     /gene="crp"
                     /locus_tag="VFB37_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016506431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
     gene            complement(5111..5803)
                     /gene="coq7"
                     /locus_tag="VFB37_16540"
     CDS             complement(5111..5803)
                     /gene="coq7"
                     /locus_tag="VFB37_16540"
                     /inference="COORDINATES: protein motif:HMM:NF033656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HZO24105.1"
                     /translation="MDPLIGAADRALRALFAPAHASRPVPEAVEQQATPGTAPPGVGD
                     ALTGQAGPPAAAPISALADDRRRSAALMRVNHAGEIAAQALYHGQALASRSEATRELL
                     LRAAREESDHLAWCETRLRELGSRPSLLNPLWYAGSFAIGALAAVLGDRVSLGFVVET
                     ERQVEGHLDEHLTRLPEADTRSRAILQAMRTDEIAHGAAARSAGATDLPSPVSTLMRQ
                     TARVMTSTAYWI"
     gene            5992..6453
                     /gene="rplM"
                     /locus_tag="VFB37_16545"
     CDS             5992..6453
                     /gene="rplM"
                     /locus_tag="VFB37_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZO24106.1"
                     /translation="MNEHSNLIPTKTVFAKPGTIRGDWYLVDASDQTLGRLASQIAHR
                     LKGKHKADYSPHVDMGDHIVVVNAGKIRVTGRKLTDKIYYHHTGYIGGIKSIALEKLL
                     SEHPERAIEFAVKGMLPKNPLGRRMFRKLHVFAGAEHPHRAQQPRPLEIRK"
     gene            6509..6907
                     /gene="rpsI"
                     /locus_tag="VFB37_16550"
     CDS             6509..6907
                     /gene="rpsI"
                     /locus_tag="VFB37_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZO24107.1"
                     /translation="MPASPQSNYGTGRRKTATARVYLKPGSGRIMVNERSLDEFFGRE
                     TGRMIVRQPLEAVQLISKFDITVRVDGGGITGQAGAIRHGITRALIEYDESLRKPLRD
                     AGFVTRDAREVERKKVGHRKARRGPQYSKR"
     gene            6985..7058
                     /locus_tag="VFB37_16555"
     tRNA            6985..7058
                     /locus_tag="VFB37_16555"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7017..7019,aa:Gln,seq:ctg)
     gene            complement(7401..8063)
                     /gene="rpiA"
                     /locus_tag="VFB37_16560"
     CDS             complement(7401..8063)
                     /gene="rpiA"
                     /locus_tag="VFB37_16560"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HZO24108.1"
                     /translation="MSTDSEQAKRNAARAALDYLESGTVLGVGTGSTVNLFIEILASH
                     RDRVHAAVSSSNASTERLKAAGIQVLDLNIVTELGVYVDGADEATRARQLIKGGGGAL
                     TREKIVAAAAARFVCIADESKLVDRLGAFPLPIEVIPMARSLVTRTLQRRGARPMWRK
                     GFVTDNGNDILDVSDLKIDDPPGLERELNQIAGVVTVGLFAIRPADVLILGSSHGVEI
                     IS"
     gene            complement(8314..8778)
                     /locus_tag="VFB37_16565"
     CDS             complement(8314..8778)
                     /locus_tag="VFB37_16565"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZO24109.1"
                     /translation="MHYWLMKTEPGTFGIDHLAAAPRATTAWDGVRNFQARNMLRDGM
                     KLRDQAFLYHSSCDVPGIVAVMEIVREGYPDPTAFDRKHHHYDAQSDPAKPRWYMVDV
                     RLKKRLARIITLDELRKHAAKELKGFVLLRPGNRLSVMPVEPAHWRYVLSLE"
     gene            complement(8763..9419)
                     /locus_tag="VFB37_16570"
     CDS             complement(8763..9419)
                     /locus_tag="VFB37_16570"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZO24110.1"
                     /translation="MLLWFESNGSHRHLRVTLPDPTFAAARKSLRAALRARRRALDSS
                     ERTSSAQRVARNADGWLRLRPGWRIALYASLAEELDTAPLLAIARIRGCRIYLPRIDR
                     HRHARGMRFVEMTDRQRTNRLGICEPEGMRVIGARWLDVVFLPLVGFDARGVRLGAGG
                     GYYDRAFSFRRWRAAWHTPHLVGLAYAFQQVEAILPAAHDVLLDAVITEKGVIRCTTG
                     "
BASE COUNT         1710 a         3102 c         3086 g         1623 t
ORIGIN      
        1 cagcaacgcc gtcacgatga atgcgaatcc ggcgtacacc acggtgagtc gggtcgagta
       61 gaccacgaac gcggtaaaca tcttgccgac ggccgcccat agcacgcccc cggcggccgc
      121 cccgaccagg gcgggtcgcc agcgcacccg cgtgttggga atgaacatgt acatgccggt
      181 gaagaaggcg gtgaccatca cgtagggggc cagcgcgagg gcaattccgt gcagcagctg
      241 aagcagcggc agcttcgcca tcgtctggag cgggacgctc gcaatggcgg cgtgcgagag
      301 ccccacgaac gctaccagca ggaccggacc cacaatgagc aatgtcaggt attcggcgat
      361 acggcgcgcg aagctgcgcg gctgctccac gcgccataga aaattgaagc tgtcctccac
      421 cttcttgatc gtcccgagca aagtccaggc gagcagggcg aagcccaccg atccaacgat
      481 gccgctgctg acacgatgcg cgaactgcag aacgtgctcg gtgagctcct gagccgcctg
      541 gccgccgacg gggcggaaga actcgtacac gatcggctcg agatcccgac gcgcaccgaa
      601 aaccttgagg atggcgaagc tgaaagcgag cagcgggatc agcgacagca gtgtcgtgta
      661 caccaggctc atcgcgcgca ggttgatggc gccccgccac agatcgcgta ccactgcgta
      721 cgggtagcgc aggccgcgaa ccagccgggc cggcaccccg ggcccggccg aggccggacc
      781 gaagaagcac caatcgagaa aagtccagat gcgcgcccac atggcggagg gtttcctacg
      841 ctcgagacgg gtatgcccgc acagagtagc accctggctg gagccgtcgc cgctgacaga
      901 ccggagctgc tcggctacag tccgaggggt atgtcagcgc agtttccgca gcgcatcgtg
      961 tgcctgacgg aggagacgac cgagaccctc tacctgctgg gggaggactg gcggatcgtc
     1021 ggtatctcgg gcttcacagt cagaccgccc cgggcgcggc gcgagaagcc cagggtctcg
     1081 gcattcacga gcgccaaaat cgatcgcatc gtggccctgc acccggacct ggtgttgggc
     1141 ttctccgacc tgcaagcgga catcgcagcg cagctggtgc gcgccgggat cgaggtgcat
     1201 gtgttcaatc atcgcagcgt cgaggagatc ctgcgcatga tccgcacgct gggcggcatg
     1261 atcggccagc aagagaagac ccgcgcgctg gtggagcggc tggccggcgg tctccaggag
     1321 gtacgcgagc ggagcgccgg cctggaacac cggccgcgtg tgtatttcga agagtgggat
     1381 gcgccgcaga tctccggtat ccgctgggta tccgagctca ttgcggtggc ggggggcatc
     1441 gattgttttc cggagctcgc cagccaatct ctcgcccgcg atcgcatcgt ccaggaccct
     1501 ctggaggtag cgcgccgcgc gcccgacatc attctcggat cctggtgtgg caagcgcttt
     1561 cggcccgatc aggttgcggc acgcccgggc tgggatcgga ttccggcggt gcgcaacggc
     1621 tacgtgcgtg agatcaagtc gccgctgatt ctgcagccgg ggcccgccgc cctgaccgat
     1681 ggagtgcgcg ccattcagcg catcatcgag gaatgggaga gtggtgggcc cgcactgatg
     1741 agccaggccg ggttacacgg cgacgcttga actagagacc gcggcgctcg cgaatgacct
     1801 cataggcttc gatgatctgc tgagtgcgct gcttggcgtg ctcaatcatg gactccggca
     1861 gcccgttcgc cttgagcttg tcgggatggt gccggctcag ctggcgccgg tatgccttca
     1921 cgacctcctc gtcgctcgcc tgcggcgcgc attcgaggac actgtaggcc tgctggagct
     1981 gtatcgcgct gttctggcgc gccggttgct ggcgaaaact tccgctgcgt atgcgcagga
     2041 ctgcttcgat ctgcgcgagc tccaccatgg agacgccgag ccggcttgcc attcgcccga
     2101 gcagcacacg cgaggggcct tcgaggttgc tgccggcgag cgccgctcgc acctggatct
     2161 ccatgaacac gcgcagcagg tccggccttc cgcggcaggt ggcggcgagc gcagcgagct
     2221 ccgctgcgag gttgaattcg ggctgcttgc ctgcggtgaa gtactcgatc gcggactgca
     2281 cctgcgcagg cgtgagacgc agatccgtca tcactgcgcg ggcggcggcg atctcctgct
     2341 cgctgacacg accgtcagct ttcgcaacgt gccccatgac acgaaaggcg gcgcgaaaga
     2401 actgatcacc gatcgcgctc gcatccgcat cgtgctgcag gcccgcatca ttgcccggag
     2461 cgctggagta ctcatcgaac tggtgtccca ggagcacgcc gagcgcggcg ccgatcggcc
     2521 cgagcgccag catgccgatc gccccgccca ccactttgcc ggtgaatttc atgcgctgct
     2581 atagtaccaa gctcaccccg caccccggtc ctgccctgca tgccctcgcc aaccgtctac
     2641 cgcacagctt tgcgcggact cgagaagatt catgagggca aggtgcgcga tatctatgcc
     2701 gtcgatacgc agacgatgct gatcgtgacg accgatcgcc tgtccgcctt cgacgtcgtg
     2761 ctgccggacc ccattccgga caaggggcgg gtgctcaatg agatttcgag cttctggttc
     2821 tcgcgcacgg ccgacatcgt gccgaaccat ctcacggacc ggccgctcga gagcgtgatc
     2881 accgatgcca atgagctggc cctgctcgcg ggtcgcgccg tgctggtacg gcgtctggcg
     2941 ccgctgccga tcgaggcagt cgtgcggggc tatctgatcg gctcgggatg gaaggactac
     3001 cagaagagca gaagcgtatc ggggatcgaa ctcccggcag gcctgcggca ggcggagcgc
     3061 cttccggagc cgctgttcac acctgccacc aaggcggcgc ccggcgagca tgacgaaaac
     3121 atcgcctttg cgcaggttga gcagatggtc ggtgctgccg tcgcaaagcg ggtccggcaa
     3181 gttgctctcc agctctacaa cttcgcagtg gagcacgccc gcaagcgcgg catcatcatt
     3241 gccgacacca agttcgagtt cgccctggac tcggcgggca atctgacatt gatcgacgaa
     3301 gcgttgacgc cggattcgtc ccgtttctgg cccgccgaca cttaccgcgt cggcgcgagc
     3361 ccgccgagct tcgacaagca gttcgttcgc gactacctcg agaccctgga ctggaacaaa
     3421 aaggcaccag gcccggccct gcccgagcag atcattgcgc gcaccagtga gaagtaccgc
     3481 gaagccctca agcggctgac ggcgccggtg cccacggcga cctgagcgga cactgcaggt
     3541 acactgcact catgcaaacc ccctggattg cgccctcgat cctgtcggcc gattttgccc
     3601 ggctgggtga ggaggtgacc gcagtcctgc gcgccggcgc cgatgtcgtc catttcgatg
     3661 tgatggacaa tcactacgtg ccgaacctga cgatcggtcc gctggtctgc gaggccctgc
     3721 gccagtacgg catcgaggcg ccgatcgacg tccacctgat gatccggccc gtggaccgca
     3781 tcgtgcccga cttcgcagct gcgggagcga ccttcatctc attccatccc gaggcgaccg
     3841 atcacgtcga tcggaccatt gagctcatac gcgagcacgg ttgcaagccg ggcctggtat
     3901 tcaatccggc gacgccgctc gactatctcg actacaccct ggagaagctg gacatggtgc
     3961 tgatcatgtc cgtgaatccg gggttcgggg gtcagaagtt cattcccgcc gccctgcaga
     4021 agctggccga gacccggcgg cgcatcgatg cgtccggccg ggcagtgcgg ctcgagatcg
     4081 atgggggcgt gaaggtggac aacattggcg cgatcgcgcg ggcgggcgcg gacaccttcg
     4141 tcgcaggatc ggcgatcttc gaaagtgatg actacgcgaa gacgatccgc gcgatgcact
     4201 cccacatcga ggaagcgcgt cggccaggct agacggggtc agtcctcgtc gtcgtcatca
     4261 tcgtcatcat cgtcatccag cgcggccggg ctgctcgcag ccttcgctgc ggcgggcttc
     4321 gcaggcacga tgacgggacg cgtcttcatc ttcgggcgag cgttgaagac cacgatcgtc
     4381 ttgccggtcg cgcgcagcag cccctgttcc tccagcacct tcagcacacg cccagccatc
     4441 tcgcgtgagc agcccactag gcgtgagagc tcctgccggc tgaccttgat ctgcatgccc
     4501 tcggggtggg tcatggcgtc gggctcgccg cacaaatcca taatcgcgtg cgccacacgg
     4561 ccggtgacgt ccacgaaagc gaggtcgccg agcttgcggt tggtacgatc caggcgcgtg
     4621 gccagctgcg tggcgagctc gaagatgagg ccggggctct cggcggcgat ctggcgaaag
     4681 cgcgggtagg tcatctcggc cagctcgcac tgattgcggg ttcgcaccca ggcgctgcgg
     4741 gttggctgct cgtagaacag gcccatctcg ccgaagaact gtccccggtt gaggtaggct
     4801 agcaccatct cgttgccttc ctcgtcctcg atcatgacct cgaccgagcc gctgatgatg
     4861 tagtagagga catccggcag atcgcccgcg tggatcacta ccgtcttcga cggtacggtc
     4921 cggatccggc aataactcag gaagcggttg atagccggga tatcgctcaa cggtttgatg
     4981 ttttcttcca tcacggctcc ccgaatggcg ccattgcacg gcggcccgct cttttataca
     5041 taagcctgca gaactgcaag cgctgcgcag ccggcgcgct cgtttgtgga ttgcagcagc
     5101 cgccttcgcc tcaaatccag tacgccgtac ttgtcatgac gcgggcggtc tgtcgcatca
     5161 gggtgctcac cggcgatggc agatcagtgg cgccggccga tcgggccgct gcaccgtgcg
     5221 cgatctcatc cgtcctcatg gcctgaagta tcgcacggct gcgcgtgtct gcttccggca
     5281 gcctggtcag atgctcatcg aggtggcctt ccacctggcg ctcggtctcg accacgaatc
     5341 cgaggctcac gcgatcgccc agcaccgctg cgagcgcgcc gatggcgaac gagcctgcat
     5401 accacagagg gttgagcagg ctgggtcgcg acccgagctc ccgcagccgt gtctcgcacc
     5461 aggcgagatg atcggactcc tcccgggcag cgcgcagcag cagctcgcga gtggcttcgg
     5521 agcgggaggc caacgcctgg ccgtgataaa gcgcctgggc ggcgatctca ccggcgtgat
     5581 tgacgcgcat cagcgcggcg gaacgccggc gatcatcggc cagcgcactg atgggtgcgg
     5641 ccgccggcgg ccctgcctgc ccggtcaagg catcgcccac gcccggtggc gcagtgccag
     5701 gggtggcctg ctgttcgacg gcctccggca ccggccggga ggcgtgggcc ggggcgaaga
     5761 gggcccgcag cgcgcgatcg gcagccccta tgagggggtc caaaggatgt ccggagtcca
     5821 ttggcggcat tataagccca ggcggtgggg gcttgtgggg cgtggctttg cattgccgga
     5881 cccgcttgag tacacttccc cgcctttttt cggctccgac cgcgcacaag cgcaggcgcg
     5941 gccgacactc gcggtgcgat ccgccgcgcc ttgcatgcag ccgtgatccc catgaacgag
     6001 cactcgaacc tgattccgac caaaactgta tttgcgaagc ccggcacgat ccgcggcgac
     6061 tggtacctgg tcgatgccag cgaccagacc ctcgggcgtc tcgcctcgca gatcgcgcac
     6121 cgcctgaagg gcaagcacaa ggccgattac agccctcacg tggacatggg cgatcacatc
     6181 gtcgtggtga atgcgggcaa gatccgcgtc accggtcgca agctcacgga caagatctac
     6241 taccaccaca caggctatat cggcggcatc aagtcgatcg cgctggagaa gctgctgagc
     6301 gagcaccccg agcgggccat cgagttcgcc gtgaaaggga tgctgccgaa gaatccgctg
     6361 ggacggcgaa tgttccgcaa gctccatgtg tttgccggag ccgagcatcc gcatcgcgca
     6421 cagcagccgc gtccgctcga gatcaggaag taagcgcccg ccgcgactgc accccgtagc
     6481 gcccgcatca ttcgaggact ctcaatacat gcccgcctct ccgcaatcga actatggcac
     6541 cggccgccgc aagacggcga cggctcgcgt ctacctgaag cccggcagcg gccggatcat
     6601 ggtgaacgag cgctcgctcg atgagttctt cggacgcgag accgggcgca tgatcgtgcg
     6661 ccagccgctc gaggccgtgc agctcatctc caagttcgac atcacggtga gagtggatgg
     6721 cggcggcatc accggccagg ccggagcgat ccgccacggc atcacgcgcg cactgatcga
     6781 gtacgacgag agtctgcgca agccgctgcg cgatgcgggc ttcgtgacgc gcgatgctcg
     6841 cgaggtggag cgcaagaagg tcggccatcg caaggcccgc cgcggtccgc agtattcgaa
     6901 gcgttgaggt tcagccagcc ggggcccaag gcgtgccttt cgcccgcgca ctgaacgcag
     6961 ctgtcccggt tgccaatgca agagtggggg atcgtctagt ggtaggacaa cggactctga
     7021 ctccgtttac ctaggttcga atcctagtcc cccagccaaa catgcgagag gcccgccgct
     7081 ggcgggcctc ggcatttttg gcaacggaac tgggataacg accgccaggt cggcccgaac
     7141 gggcgcggcg caccgcgcag cggcgccgcg taatcctagt cccccagcca aaccacgcag
     7201 gcccaccaat ggtgggcctg tgtgtttgaa gagcaggccc gcgacgccgg ggtccccggc
     7261 aaatcgagcg tcttcacgat cggtgcgtgg ccgctcagcg cctcaagctc gattttccgg
     7321 ggtggtcccg cgggcctcgg catttttggc aacggaactg ggataacgac cgccaggtcg
     7381 gcccgaacgg gcgcggcgca tcagctgatg atctcaacgc cgtgcgaaga gcctaggatc
     7441 agaacgtccg ccggacgaat cgcgaacagg cccacggtca ccactccggc aatctggttc
     7501 agttcgcgct cgaggccggg aggatcgtcg atcttcagat cgctcacgtc gaggatgtcg
     7561 ttgccgttgt cagtgacgaa ccccttgcgc cacattggac gagcgcctcg acgctggaga
     7621 gtccgggtga ccagtgagcg cgccatcgga atgacttcga tcgggagcgg gaacgcgccc
     7681 agccgatcca cgagcttgct ctcatccgcg atgcagacga agcgggccgc ggccgctgcc
     7741 acgatcttct cccgcgtcag cgcgcctccg cctcccttga tcagctgacg cgcgcgggtc
     7801 gcctcatcgg cgccatccac gtataccccg agctcagtca cgatgttcag atccagcacc
     7861 tggataccgg ccgccttgag acgttcggtg gaggcgttgg aactggagac cgctgcgtgc
     7921 actcggtccc ggtgcgaggc gaggatttcg atgaacaggt tcaccgtcga tccggtaccg
     7981 acgcccagga cggtaccgct ctcgagataa tccagcgcgg ctcgcgccgc attccgcttt
     8041 gcctgctcgc tgtcggtgga cactttgccg cctccagaaa tgactgtgcc cgcttgcgcg
     8101 ggcacagtct cctcgaagtc cactcggcgg ccgcgcaagc ggccgccgag ttaggcctat
     8161 ttcttctttg cagctttctt cttggccttc ttctttgctt tcgccatgtt ggctctccag
     8221 ttaacgggtt agtccgaggt cggagtatat agatgcgaaa tgaaattaca agcgactgcg
     8281 aaatgtgcta ggcacttccc gcaacgaccc gactcactcc agagagagga cgtagcgcca
     8341 gtgagcgggc tcgacgggca tcactgacag gcgattgccc ggacgcagga gcacgaaccc
     8401 cttcagttcc ttcgctgcgt gcttgcgaag ctcatccagc gtgatgatgc gagcgaggcg
     8461 ctttttgaga cgtacgtcca ccatgtacca gcgcggcttc gcaggatcgc tctgcgcatc
     8521 gtagtgatga tgcttcctgt cgaaggccgt cgggtcgggg tagccctccc gcacgatctc
     8581 catgactgcg acgatacccg gcacatcgca gctcgagtga taaaggaaag cctggtcgcg
     8641 cagcttcatc ccgtcccgaa gcatgttgcg cgcctggaaa ttgcgcacgc cgtcccacgc
     8701 tgtcgtggcg cgcggcgctg cggctagatg atcgatgccg aatgtgccgg gctcggtctt
     8761 catcagccag tagtgcatcg tatgacccct ttctcggtga tcactgcatc gagcaataca
     8821 tcgtgagcgg ctggcaggat cgcctcgacc tgctggaatg cataggcgag gcccaccaga
     8881 tgcggcgtat gccacgctgc tcgccagcga cggaacgaga aggcgcgatc gtagtagccg
     8941 ccgccggcac ccagccttac accgcgggca tcgaagccga cgagcggcag gaacacgaca
     9001 tcgagccagc gggcgccgat gacccgcatg ccctcgggct cgcagatacc cagtcgattg
     9061 gttcgctgcc gatcggtcat ctcgacgaag cgcatgccgc gagcgtgccg gtggcgatcg
     9121 atccgcggca gatagatgcg gcaaccacgg atgcgcgcaa ttgccaggag cggcgcggta
     9181 tcgagctctt cggcaagtga ggcgtacagc gcaatgcgcc aacccggtcg gaggcggagc
     9241 cagccgtccg cattgcgagc gactcgttgt gcgctcgagg tccgctcgct gctatcgagg
     9301 gcgcgccgcc gggcacgcag cgctgcccgc aaggatttgc gggcagcggc gaaggtggga
     9361 tcgggtagcg tcacccgcag gtgccggtgc gaaccgttgc tctcgaacca gagcaacaag
     9421 tgggacgaac tgcagcaggt aaggctttcc gctcgaagcg gacttgcaca attctgcccg
     9481 caagtcctga tgtcccgggg tgtttcaatt agggtccgag g
//
LOCUS       DASRHN010000409         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2296143, whole genome shotgun sequence.
ACCESSION   DASRHN010000409 DASRHN010000000
VERSION     DASRHN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296143"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFB37_16575"
     CDS             complement(<1..1724)
                     /locus_tag="VFB37_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24111.1"
                     /translation="MASASNASRAETSSAAASSAGTGSGSNIRPAVDVIAITTRDDFL
                     LELGESLGGQASVRPVDSMPVALEHLSNSKRGQVLVIDTRDVSDIRSDIERAHLQAGH
                     AVVLVFTVPETEKQVGMAVKGTNVFAVLPIPIDKRKTGAVLEGAMADAVARKSMRGSE
                     RTNVSIEPFQAQVDSGSSSDSGGRSKMGLFVGVGIAVVAIAAGAYMFLGKSKDTGAPT
                     GAAPPAAGASTNIAPTLAVKPTEESAAPAVETSLVKGKVDDLLEKARLAMRERRYSEP
                     IGDNALLYYRSAAAADPSNGEASDGLQRVASVLASRFDEAMNGGRFDEASLALANFKA
                     AAPSDSRIAALEVRLITAQISKALADGNLDRVNQLIRQAQSSPAITADQINKWKAEAN
                     RRQEDAKVQRLAALITDRIRDGRLIDPADDSAKMYLQQLHDLAPTNATTQRLNRDLNA
                     AYMRKARESAIANHAPDVDRWLAEAKAGGVGAGEINSFQKELSNARQKAVTAEADRMA
                     GLVRERLRDGRLTDPAQDSAAYYINQIQGMDSSNANIAPLSRDLASKLVERARLAAQT
                     GKGGGVVD"
     gene            2072..>3074
                     /locus_tag="VFB37_16580"
     CDS             2072..>3074
                     /locus_tag="VFB37_16580"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZO24112.1"
                     /translation="MRVLRVVAVVVAGLLGLLIVALIAVLLFVDPNKYRGQIEQAARQ
                     HTDRELTIGGKLQLRFFPWLALSVGDARLSNRPGFGTEPFLTVRNASIGVAVLPLLGG
                     RLEVSRIRLEGVSLNLVSRGAQNNWQDLSESDESEQTQTAAKPVGPAPSKGAEGAEAS
                     RKPGTRGHLSVEGMDLSRFSVVYRDEVKKTTTEISNAELHMAALDVGSQRVAMGKLEL
                     EGTYLARGVASPASAEAGHSPEEPKPLSFALRTSALTLDGVTEILSPAKAELKVGDSA
                     LTISVSGEKMSSDPQVSGTVTIPNTSARKLLQSLGIAPPVTRDPQALAIVGLQTNFRL
                     "
BASE COUNT          549 a          962 c          977 g          586 t
ORIGIN      
        1 ctgtccacca ccccgccgcc cttgccggtc tgagccgcga gccgggctcg ctccaccagc
       61 ttgctggcga gatcgcgact caagggcgcg atattcgcat tgctgctgtc catcccctga
      121 atctggttga tgtaataagc agcactgtcc tgcgcagggt ctgtcagacg cccatcgcgc
      181 agacgctcgc gaacgaggcc cgccatccga tcggcctccg cggtgaccgc tttctgacgg
      241 gcattcgaga gctccttctg gaaggagttg atctcgcctg cgccgacacc gccggccttt
      301 gcctcggcca accatcggtc cacgtcaggc gcatggttgg cgatcgcaga ttcgcgtgcc
      361 ttgcgcatgt acgcggcgtt cagatcacga ttcagccgct gagtcgtggc gttcgtgggc
      421 gcgaggtcat gcagctgctg caagtacatc ttggcgctgt cgtccgcagg gtcgatcagc
      481 cgtccatcgc ggatacggtc cgtgatcagt gccgcgaggc gctgcacttt cgcatcttcc
      541 tgacggcgat tggcctcagc cttccacttg ttgatctggt ctgccgtaat ggcaggggag
      601 gattgcgcct ggcggatcag ctgattgacg cggtcgagat taccatccgc cagtgccttg
      661 gaaatctgcg ctgtgatcaa ccgaacttcg agcgctgcga tgcggctgtc gctgggagca
      721 gccgctttga aattggccag cgcgagactt gcttcatcga agcggccgcc attcatagcc
      781 tcgtcgaagc gggatgcgag cacgctcgcg acccgttgca gtccgtcgct ggcctcgccg
      841 tttgacgggt cggcggcagc cgcggagcgg taatacagca gggcgttatc gccgatcggc
      901 tcggaataac gacgctcccg catggcgagg cgggcttttt cgagcagatc atcgacctta
      961 cctttcacca gtgaggtctc gacagcgggg gcggcggatt cctctgtggg cttcacggcg
     1021 agagttggcg cgatgtttgt gctggcgccc gctgccgggg gcgctgcacc ggtcggagcg
     1081 ccggtgtcct tggacttgcc gagaaacatg tacgcgccgg ccgctatcgc cacgacggca
     1141 ataccaacac cgacgaacag acccatcttg ctcctgccgc ccgagtccga cgatgagcct
     1201 gaatccacct gagcctggaa cggctcgata ctgacgttcg tgcgctcgga gccgcgcatc
     1261 gacttacggg ccacggcatc cgccatcgcc ccttcgagaa ccgcgccggt cttgcgcttg
     1321 tcgatcggga tgggcagcac cgcgaatacg ttggtgccct tgacggccat gccgacctgc
     1381 ttctccgtct cgggaaccgt gaacaccaac actacggcat ggccggcctg cagatgagca
     1441 cgctcgatat ccgagcggat gtcgctgaca tcgcgcgtgt cgatgaccag cacttgcccg
     1501 cgcttcgagt tggagagatg ctccagcgcc accggcatcg agtccacggg tcgcacggat
     1561 gcctgtccgc ccagggactc accgagctcg agcaggaaat catcgcgggt cgtgatggcg
     1621 atgacatcga ccgctggccg gatgttggat ccggagccgg ttcccgcgga actggctgcg
     1681 gcgctgcttg tctcagcacg agaagcattc gatgcgcttg ccacagagtg tgtctcctgt
     1741 atcgcgggcg tccaggcccg cggaagtgcg acagtcctca tgcccctggg accgctagca
     1801 tgaggagcca cgtgaaattg gagcggtacg gaactgtcat ggcagcatag tttgctgctg
     1861 cagcaccgca atgccacagt tcacaataat gagagtgggg cgtctcttgc tgcagacgca
     1921 cggacaaacg tctagagcta cctcgcgttt tccgtaactc acgaccgagc tgggatggtg
     1981 attttgtgcg ctgcgcccct gttcccgcaa caagtgtcac ctatgggaga ctggataccg
     2041 atgcgccagg gacataagaa aggggtctag catgagggtg ctccgggtcg tcgctgtggt
     2101 ggtggcagga ctgttagggc tactgatcgt tgccctgata gccgtgctgc tgttcgtgga
     2161 tcccaataaa tatcgcggcc agatagagca ggccgcccgg cagcacacgg accgcgagct
     2221 caccatcggc ggcaagctgc agctgcgttt ctttccctgg ctggcgctgt cggtcggcga
     2281 cgcccggctc agcaaccgtc cggggttcgg cactgagccc ttcctgactg tgcgcaatgc
     2341 cagtatcggg gtggcggtcc tgcccctgct cggcggacgg ctcgaggtca gccggatccg
     2401 gctggagggc gtcagcctca acctggtgag tcgaggcgcg cagaacaact ggcaggacct
     2461 ttccgagtcc gacgagtcgg agcagacgca gaccgcggcc aagccggtgg gccccgcccc
     2521 atccaagggg gccgaagggg ctgaagcgtc ccgaaaaccc ggcactcgcg gccatctttc
     2581 cgtggaaggc atggatctca gcaggttttc tgtcgtctat cgcgacgagg tgaagaagac
     2641 gaccacggaa atcagcaacg ccgagctgca catggccgcc ctggacgtgg ggtcacagcg
     2701 agtcgcaatg gggaaactcg agctggaggg tacctacctg gcaagaggcg tggcatcccc
     2761 agcgtccgct gaagctgggc actcccctga ggagccgaag cctctctcat tcgcgttgcg
     2821 cacgtcggcc ctcacactgg atggcgtcac cgagattctc tcgcccgcaa aagccgaatt
     2881 gaaggtgggc gattcggctc tgacgatttc cgtcagtggt gaaaaaatga gcagcgaccc
     2941 ccaggtgagc ggtactgtca caatcccgaa tacatcggcg cggaagttgt tgcaatcgtt
     3001 gggtatagcg ccgccggtga cccgcgaccc gcaagcgctc gcgatagtcg gcctccagac
     3061 caactttcga ctga
//
LOCUS       DASRHN010000410         5615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2451165, whole genome shotgun sequence.
ACCESSION   DASRHN010000410 DASRHN010000000
VERSION     DASRHN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451165"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFB37_16585"
     CDS             complement(<1..910)
                     /locus_tag="VFB37_16585"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZO24113.1"
                     /translation="MQASLTSTSGRPAWALEVLGTAMVYCAVARLGLELAVQARQVSL
                     IWPATGVALACLVLRGRRLWPAILLGAFAANLLNDTPVLASLGIAVGNTLEAFLAATL
                     LQRAGFEPSLRRLHDVASLFLLAAFLCTLASATIGVLSLCLTGVTPWSSFTSLWSTWW
                     IGDAIGNIVTAPFLFVWADQVRNRSPLRRLGEAALLLGCMLLLSLMIFVDHGTTTPYP
                     VHYLIFPAVIWAALRLGQQATATLVFGAMSLTVIATLAGRGPFLTWDIGASLMQVALF
                     MAVVAVTGLFLGAATAERNRAERRRTE"
     gene            1040..2395
                     /locus_tag="VFB37_16590"
     CDS             1040..2395
                     /locus_tag="VFB37_16590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO24114.1"
                     /translation="MSTSAQGDSALPASRAATIIQRLVVVHPGELPALLASFATLFCM
                     FSSYTILRPIRDTMGVTSGLQSLPYLFWGTFIVMLVLQPVYGWLTSRLRRPVFLPWVY
                     AFFTANLVAFYVWFHVQQDHTWIARTYYVWVSVFNFFIVAVFWSLMADVFTREQAGRM
                     FGFIAAGASTGGLVGPLLASRLAVPLGTINLLLISSGLLAASLIFMTRVLHWHRDHGA
                     AARGAATDKPLGGSVLAAFTQVVRSPYLLGIALFVILLSWVSTFLYLQQQAFVAKVFT
                     SADERTRFFSDVDFWVQCCSLFTQLFLFGRLFRRVGLRVMLVSVPLLMTVGYALFALV
                     PTFAVLVGVLAIRRVAEYAITRPCRDSLFTVCAREEKYKAKSLIDTFIYRGGDATSAS
                     LYKLLTHGLGLGATGIGWAGAVIAGVWALLALALGRSQERAARLSLESAYVDPEESGR
                     A"
     gene            complement(2381..3832)
                     /locus_tag="VFB37_16595"
     CDS             complement(2381..3832)
                     /locus_tag="VFB37_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO24115.1"
                     /translation="MAKKHNVPSRLAVADRDTRYAEWLRHHLGVLCPDAAVNTLDHSE
                     FDHWSAKLTRRDCDVLILCASFGSSPEDPRAKGLELLRRLREHPAFPVVIAVAEEGNE
                     LTAVRALQLGAVDYLPKRLLTPDRLKTAVRLALRRIEKRVARRIARLARAAAAHPLEA
                     KSKSPELEHAQGEATSGAAPAALEAGSAADRGPTGASPSAASANVPGGDFMSADFIPG
                     YTIHATIGESEKAAVYVATSLARQQNVAVKLSKTLRDDTAGRQFLEREYTAIAAIHDP
                     CVVQIYDYGVHAGYEYLVMEYLPRGDLKSRMQKGLTEQEALRYVERIAAALQIVHQAG
                     LIHRDLKPPNIMLREDDEVALIDFGLARALDGSTPSTRTGVLRGSPYYMSPEQALGEP
                     LDTRSDFYSLGIMYYEMLTGKKPYTGGSAMDVLQQHVNAPVPQLPHALAQHEPLLGRL
                     LAKAREERFGSAAEILAAIGSLRNEATAQSSAA"
     gene            complement(3832..5442)
                     /locus_tag="VFB37_16600"
     CDS             complement(3832..5442)
                     /locus_tag="VFB37_16600"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZO24116.1"
                     /translation="MASLSLKHTTDEAPIAVDLPRGLGLRVESLVVLSREAALAQAVK
                     GLGSEHDVLTIDAEERLDEALLGRHTGVAVLDAAAATGPIEVLTERLRAQFPELVLIV
                     AGGHEHQSALARQITNGTVYRFLHKPVSEPRVKLFVEAAWRRHSAEQAEASDVRAATG
                     TYPRPGQPPSRIGLVALGIALSVLVALGVWYATRKPDAPQAVAAPETARATNDPQAQV
                     ASQIDRLLSAAETQLGAQHLEEAQNLIAQARALKPDHVRVAFLAARLAKERERAQLAQ
                     ARLAALAAQAHRLEQQKLAEQVTDDLAKAADRLRAGQIIEPAEDNARFFIDSARDLAP
                     GDADVRQAQSQLADRLVAEARTALSAGKAADAQHWIDAAANSGVATDTVASLTQDLQH
                     LQHPQPTVRAEAAPEPPASAVNVDTRVAAAGARVTEQKSAPTQAVAPPASEPVIAATL
                     HRTHYSAPTFPVSARERGVSGWVDIQFVVKTDGTVSDVVATGAEPVGMFEQAATDAVR
                     KWRYQPLIRDGQPVEQRARVRMTFALKD"
BASE COUNT          881 a         1846 c         1862 g         1026 t
ORIGIN      
        1 cctcggtgcg gcgccgctcg gcgcgattgc gctcggcggt cgccgcgccc aggaacagtc
       61 cggtgaccgc cacgaccgcc atgaagagtg ccacctgcat caggcttgca ccgatgtccc
      121 aggtcaggaa cggccctcgg ccggcgagtg tggcgatgac ggtgagcgac atcgccccga
      181 acaccagggt cgccgtagcc tgctgcccga gtcgcagggc cgcccagatc accgccggga
      241 agatcaggta gtggaccgga tagggcgtgg tcgtgccatg gtccacgaag atcatcaaac
      301 tcaacagcag catgcagccc agcagcagcg ccgcctcccc aagccgccgc agcggcgagc
      361 ggttgcggac ctggtcggcc cagacgaaca ggaatggggc ggtcacgatg ttgccgatgg
      421 catcgcctat ccaccacgtg gaccacagcg atgtgaaact gctccagggc gtgacgcccg
      481 tcaggcaaag gctgagcacg ccgatcgtgg cgctggcgag cgtgcacagg aaggcggcca
      541 gcagaaacag cgaggcgaca tcatgcagcc ggcgcagcga gggctcgaat ccggcgcgct
      601 gcagcagcgt ggccgcgagg aacgcttcga gcgtgtttcc gacggcgatg ccgagggagg
      661 cgagcaccgg ggtgtcattg agcagattgg cagcgaaagc acccagcagg atcgcgggcc
      721 atagccgccg tccacgcagc accaggcagg cgagcgcgac ccctgtcgcc ggccagatga
      781 gcgatacctg ccgcgcctgc accgccaact cgaggccgag cctggcgact gcgcagtaca
      841 ccatcgccgt cccgagcacc tcgagcgccc aggccggccg cccggaagtc gaagtgaggg
      901 acgcttgcat gcgtgagtct cctcgcccac atgggcggcg gatgcgaaag ctgcggcgag
      961 ccgatgttac gaagggccgg gttgcctgac aatgcgggcg gcaggttcct tacaatacgt
     1021 acagttcccg ggagcctgca tgagcacatc ggcccaagga gattctgcac tgcccgcttc
     1081 ccgtgccgcc acgatcatac agcggctggt cgtggtgcat cctggcgagc tgcctgcgct
     1141 gctggcctcg ttcgcgacgc tgttctgcat gttctccagc tacacgatcc tgaggcccat
     1201 ccgcgacaca atgggtgtca cctcggggtt gcagagcctt ccctacctat tttgggggac
     1261 gttcatcgtc atgctggttt tgcagccagt gtacggatgg ctcacctcac gcctgcgccg
     1321 gccggtcttc ctgccgtggg tgtacgcctt cttcaccgcc aacctggtgg ctttctacgt
     1381 ctggttccac gtgcagcagg accatacctg gattgcacgc acgtactacg tgtgggtgag
     1441 cgtattcaac ttcttcatcg tggcggtctt ctggagcctg atggcggatg tcttcacgcg
     1501 cgagcaggcg ggtcgcatgt tcggcttcat cgctgccggg gccagcaccg gcggcctggt
     1561 cggcccgctc ctggcgagcc gcctggcggt gccgctgggc acgatcaacc tgctgctcat
     1621 ttcctcggga ctgctggccg catcgctgat cttcatgacg cgcgtgctgc actggcatcg
     1681 ggaccacggc gccgcggcgc gtggcgccgc aacggataag cctctcggag gcagcgtact
     1741 ggctgccttc acgcaggtgg tgcgttcgcc ctatctgttg gggattgcgc tgttcgtgat
     1801 cctgctttcc tgggtgtcga cgtttctgta cctgcagcag caggccttcg tcgcaaaggt
     1861 ctttaccagc gctgatgagc gcacccgctt cttctccgac gtcgacttct gggtgcaatg
     1921 ctgctcgctg ttcacacagt tgttcctctt cggaagactc ttcagacgcg tcggcctgcg
     1981 cgtcatgctg gtttcagtgc cgttgctgat gactgtgggt tacgcgctgt tcgccctcgt
     2041 gccgaccttc gccgtgctcg taggtgtgct cgcgatccgg cgcgtggccg agtatgcgat
     2101 cacgcggccc tgccgcgatt cgctcttcac cgtctgcgcc cgcgaggaga agtacaaggc
     2161 caagagcctg atcgacacgt tcatctatcg cgggggcgat gccaccagtg cttcgctgta
     2221 caagctgctg acccacggct tggggctcgg tgcgacggga atcggttggg cgggtgcggt
     2281 cattgcaggg gtctgggccc tgctggccct ggcactcggt cgctcccagg aacgcgccgc
     2341 ccgcctgagt ctcgagtctg cctacgtgga tccggaggaa tcaggccgcg cttgattgcg
     2401 ccgtcgcctc gttgcgcagc gagccgatcg ccgccaggat ctcagccgca ctgccgaagc
     2461 gctcctcgcg cgccttggcc agcaatctgc cgagcagggg ctcgtgctgg gccagggcat
     2521 gcggcagttg cggcacgggg gcattcacgt gctgctgcag tacatccatg gccgagccgc
     2581 ccgtgtaggg cttctttccg gtgagcatct cgtaatacat gatgcccagg ctgtagaaat
     2641 ccgagcgcgt atcgagcggc tcaccgagcg cctgctccgg actcatgtag tagggcgagc
     2701 cccgcagcac gccggtacgc gtgctcgggg tgctgccgtc gagtgcgcgc gcgagcccga
     2761 aatcgatcaa ggctacctca tcgtcctcgc gcaacatgat gttgggcggt ttcaggtccc
     2821 tgtgaatcag gccggcctga tgcacgatct gcaaagcggc cgcgattcgc tcgacatatc
     2881 tcagcgcctc ctgctccgtg agccccttct gcatgcgtga cttcaggtcg ccgcgcggca
     2941 gatattccat gacgagatat tcatagcccg cgtggacgcc gtaatcgtag atctgcacga
     3001 cacaaggatc gtggatcgcc gcaatcgcgg tgtattcgcg ctcgagaaac tgacgtcccg
     3061 ccgtgtcatc gcgcagtgtc ttgctgagct tgaccgcaac gttctgctgg cgggcgaggc
     3121 tcgtcgcgac atacaccgca gccttttccg attcgccgat cgtggcgtga atcgtgtagc
     3181 ccgggatgaa gtccgccgac atgaagtctc cacccggcac gttcgccgag gccgcagagg
     3241 gtgatgcccc cgtggggcct ctatcggctg cggagcctgc ttcgagggcc gcaggcgctg
     3301 cgccgctcgt ggcctcgccc tgggcgtgct cgagttctgg ggatttgctc ttcgcttcca
     3361 acgggtgcgc ggcggccgct ctcgccaggc gtgcaatgcg gcgtgcgacc ctcttctcga
     3421 tgcgacgcaa agcgagccga acggccgtct tcaggcgatc cggtgtcagc aggcgcttgg
     3481 gtaggtagtc gaccgcgcca agctgcagcg cacgcacagc cgtgagctcg ttgccttcct
     3541 cggcaaccgc gatgaccacg gggaaagcgg gatgctcgcg cagcctgcgc agcagctcca
     3601 gccctttcgc ccgcggatcc tcggggctcg agccgaagga agcacacagg atcagcacat
     3661 cgcagtcgcg gcgggtcagc ttcgcactcc agtgatcaaa ctccgagtgg tcgagcgtgt
     3721 tgacagccgc gtccgggcac agcacgccca ggtgatgcct cagccattcg gcgtagcgtg
     3781 tatcgcggtc ggcaaccgcg agtctggaag gcacgttgtg cttcttggcc atcagtcttt
     3841 cagcgcgaat gtcatgcgca ctcgagcgcg ttgctcgacg ggttggccat cgcggatgag
     3901 aggttggtag cgccactttc gaacggcatc agtggctgcc tgttcgaaca tgccgaccgg
     3961 ctccgcgccc gtcgcgacta catcggacac ggtcccgtcg gtcttcacca cgaattggat
     4021 gtccacccag ccgctcacac cgcgctcgcg ggcgctgact ggaaaggtag gagccgagta
     4081 gtgggtgcga tgcagcgttg cggcaatgac gggctcgctc gcgggaggcg cgacagcctg
     4141 agtcggcgca gacttctgct ccgtcacccg ggcgccggca gctgcaactc gggtatcgac
     4201 gttcacggcg ctggcaggcg gttcaggcgc cgcctcagct cggacggtgg gttgcggatg
     4261 ctgcagatgt tgcaggtcct gcgtgagcga ggcgacggta tcggtcgcga cgcccgaatt
     4321 ggcagccgca tcgatccagt gctgagcatc tgcggctttg cccgcactca gcgcggtgcg
     4381 cgcctcggct acaagccggt cggccagctg actttgcgcc tgacgcacat cggcatctcc
     4441 gggagcgagg tctcgagccg aatcgatgaa gaagcgggca ttgtcttcgg cgggctcgat
     4501 gatctgcccg gcgcgcagcc gatcggcggc cttggcgaga tcgtccgtca cctgctcggc
     4561 gagcttctgc tgctcgagcc ggtgggcctg cgccgcgagc gcagccagac gagcctgcgc
     4621 cagctgcgcg cgttcgcgct ccttcgccag gcgcgctgcc agaaacgcga cccgcacatg
     4681 gtccggcttc aaggcgcgcg cctgcgcgat gagattctgc gcctcctcca gatgctgggc
     4741 gccaagctgc gtctcggccg ccgacaggag ccggtcgatc tgacttgcga cttgtgcttg
     4801 cggatcgttt gtcgcacgcg ccgtctcggg cgcggccact gcttgcgggg catcgggctt
     4861 gcgggtggcg taccacacgc ccaaagcgac gaggacgctc agggcaatgc cgagggcgac
     4921 caggccgatg cgcgagggcg gttggcccgg acgtgggtac gttccggtcg ccgcccgcac
     4981 atcgctcgcc tcggcttgtt cggctgagtg ccgccgccac gccgcctcga cgaacagctt
     5041 cacgcggggc tcggacacgg gcttgtggag gaaccgatag acggtcccgt tggtgatctg
     5101 ccgcgccagg gcgctctgat gctcgtgacc gcctgccacg atcagcacga gctctggaaa
     5161 ttgggcgcgc aacctttccg tcagcacctc gatcggacct gtcgcggcgg ctgcgtccag
     5221 cacagccacg ccggtatgcc ggccgagaag tgcctcatcc aggcgttctt ctgcatcgat
     5281 cgtgagcaca tcgtgctccg atccgaggcc cttgacggct tgtgcgagag cggcttcgcg
     5341 gctcaggacg accaggctct cgacgcgcag gcccagtcct cgcggcagat cgaccgcgat
     5401 cggtgcttcg tccgtcgtgt gcttgagaga gaggcttgcc aagggtccct cgttccacca
     5461 ccgcgctcag ggtcccggcc aaggcgccgg gcagtccacg gcagccgagg gccggcccaa
     5521 taactcagtt tagggcgcct acagccacgg cgacgatgac gcaggcatcg aatatttgct
     5581 gccggctggc gcggattctg tgaatcgcgt cactg
//
LOCUS       DASRHN010000411         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2131561, whole genome shotgun sequence.
ACCESSION   DASRHN010000411 DASRHN010000000
VERSION     DASRHN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131561"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFB37_16605"
     CDS             complement(<1..755)
                     /locus_tag="VFB37_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24117.1"
                     /translation="MRVALVTARAARGLDEDEPPLRMALQSAGCEAVTTEWDDPGVDW
                     ASYDLVLLRSAWDYAERLSEFLAWAQRTSALTPVQNPLPVVRWNTDKHYLAELSRSGI
                     AIVPSRFLEPGTDPVRELNEFLAAHEQAEIVIKPAVGAGSRDAQRHRRTHKDAPVAHA
                     RRLLEANRSVLLQPYLRRVEQDGETALMFFEGRFSHAIRKGPLLPAGGAATTTLFATE
                     TIQPRTPASDELKLAERIIAAIPHGALLYARVD"
     gene            complement(794..1606)
                     /locus_tag="VFB37_16610"
     CDS             complement(794..1606)
                     /locus_tag="VFB37_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO24118.1"
                     /translation="MDTAVKQAAAPPVRPGTVRWIGYKTIVIREYGRIMRIWGQTIVP
                     SVVAASLYFVIFGSLIGRRVGTMGGFDYMQYLAPGIIMMHVINNSYANVVSSFFGAKF
                     GKHVEEMLVSPLPNWIIVAGYATGGLVRGMLVGIAVTGIALLFTHLHVHHVLIIIAAV
                     VLTSLALSLGGFLNALFAKNFDQVNFVPVFVLNPLTYFGGVFYSVSLLPAWAQKVSFL
                     NPILYMVNAFRYGFLGVSDVNVGIAFALLIAAAVALFVVAVVLMERGTGTRE"
     gene            complement(1606..2550)
                     /locus_tag="VFB37_16615"
     CDS             complement(1606..2550)
                     /locus_tag="VFB37_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO24119.1"
                     /translation="MNALSVRGLTKTYKNGVQALKSIDLDVEQGDFFALLGPNGAGKT
                     TLIGIVTSLVNKSGGQISVFGHDIDRELEAAKACIGVVPQEINFNMFETPFTIVVNQA
                     GFYGIPRPVARQRAEKYLKQLQLWDKRDKMSRGLSGGMKRRLMIARALMHEPRLLILD
                     EPTAGVDIEIRRSMWDFLRETNARGTSIILTTHYLEEAETLCRNIAIIDDGRIVERDR
                     MSNLLRRLNTETFVLNLRDVLERAPMLEGYAAKLVDPHTLEVEISREQNLNELFRKLS
                     AAGIEVLSMRNKVNRLEEIFMRLVEGRTAAPAPAVGQA"
     gene            complement(2607..>3649)
                     /locus_tag="VFB37_16620"
     CDS             complement(2607..>3649)
                     /locus_tag="VFB37_16620"
                     /inference="COORDINATES: protein motif:HMM:NF016174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24120.1"
                     /translation="FISASYFELLRSYRRYGWIVLYLFGVSPVVCKSFLRGRHLELTD
                     LTGNTAYEPYATSLRMSDVGYRNRNQAGLHVSVNSLDEYVRDLTHAITTVHPPYEALG
                     IRDARGEYQQVNANVLQIENEYYSFIRPKRVARSGERPTKALRRAGVEYVEVRALDVS
                     AFDPVGVNQNKLRFLEAFLALCLMKSSPPIDDAEQAALDQNHVTVARRGREPGLTLWR
                     EGRAAPMRVWAQELLDSMTGICEVLDQGDPSRPYSQALAVQAAKLEDVTLTPSARLMA
                     ELQQTGESFFDLALRMSATHKAYFLELYPPNEERLADFAAQAQESLDRQAAIEAGDKG
                     TYEEYVARYFAG"
BASE COUNT          678 a         1178 c         1156 g          637 t
ORIGIN      
        1 aagtcgactc gtgcatagag gagcgctccg tgcggaatgg cggcgatgat tcgctcggcc
       61 agcttgagct catcggaggc gggagtccgc ggctgaatcg tctcggtcgc gaacagcgta
      121 gtcgtcgccg ctccgccggc cggcagcaac ggacccttgc gaatcgcgtg gctgaagcgt
      181 ccctcgaaga acatcagcgc ggtttcgcca tcctgctcga cacgacgcag atagggttgc
      241 agcagcacgc tgcggtttgc ctcgaggagt cgccgggcgt gggcgacggg cgcatccttg
      301 tgcgtgcgtc gatggcgctg ggcatcgcgc gagccggctc cgactgcggg cttgatgacg
      361 atctcggcct gctcgtgagc ggcgaggaac tcgttgagct cacggaccgg atccgtcccg
      421 ggctccagga agcggctcgg gacgatcgcg atcccgctcc tggaaagctc cgcgagatag
      481 tgcttgtcgg tgttccagcg cacgaccggc aagggattct ggactggcgt cagcgccgag
      541 gtccgctgcg cccaggcgag aaactcgctc agccgctcag cgtagtccca cgctgagcgc
      601 agcagcacga ggtcgtagga agcccagtcg accccggggt catcccactc cgtcgtgacg
      661 gcttcgcaac ctgccgactg cagcgccatt cgcagcggcg gctcgtcttc gtccagcccg
      721 cgcgccgcgc gcgccgtgac caacgcgact ctcacgggct gcgccccggt atccggccgt
      781 aggtcacgac cggctactcc cgtgtcccgg ttccacgctc catcaacacg acagccacca
      841 cgaacagcgc gactgcggcg gcaatcaaga gcgcgaaagc gatgccgacg ttcacgtcgg
      901 acacccccag gaagccgtaa cggaacgcat tcaccatgta gagaatcggg ttcaggaacg
      961 agaccttctg ggcccacgcc ggcagcaggc tcaccgaata aaacaccccg ccgaagtagg
     1021 tgagcggatt cagcacgaac accggcacga agttcacctg atcgaaattc ttcgcgaaca
     1081 gcgcgttgag gaagccgccg agggagagcg cgagcgaggt gagaaccacc gcggcgatga
     1141 tgatcaacac gtgatgaacg tgcagatgcg tgaacaggag cgcaatgccc gtgaccgcga
     1201 tgccgaccag cattcccctg acgaggcctc cggtcgcgta acccgccacg atgatccagt
     1261 tcggcagcgg tgagaccagc atctcctcga cgtgcttgcc aaacttggcg ccgaagaacg
     1321 aggagacgac gttggcatag gagttgttga tgacatgcat catgatgatg ccgggggcga
     1381 ggtactgcat gtaatcaaag ccgcccatcg tgcccacgcg ccggccgatg aggctgccga
     1441 agatgacgaa gtacagcgaa gctgcgacca ccgagggcac gatggtctgg ccccagatgc
     1501 gcatgatgcg cccgtactcg cggatgacga tcgttttgta gccgatccag cggaccgtgc
     1561 cgggcctcac cggcggcgcc gcggcctgct tgaccgccgt atccatcaag cctgtcctac
     1621 cgcaggggcg ggggccgccg tgcgtccctc gaccagccgc atgaagattt cctcgaggcg
     1681 attcaccttg ttgcgcatgc tcagcacttc aatccccgcc gccgagagtt tccggaacag
     1741 ctcgttcaga ttctgctccc gcgagatctc cacctccagc gtgtgcggat ccaccagctt
     1801 ggccgcatag ccttccagca tcggcgcgcg ctcgagcacg tcgcgcaggt tgagtacgaa
     1861 cgtctcggtg ttcaatcggc gcagcagatt gctcatccga tcgcgctcca cgatgcgccc
     1921 gtcatcgatg atcgcaatgt tccggcagag agtctcggcc tcctccagat aatgcgtcgt
     1981 gaggatgatc gacgtgccgc gcgcgttggt ctcgcggagg aagtcccaca tggagcgccg
     2041 gatctcgatg tccacgccgg ccgtcggctc atccaggatc agcaggcgtg gctcgtgcat
     2101 gagcgcacgc gcaatcatca ggcggcgttt catgccgccg gaaagaccgc gtgacatctt
     2161 gtcgcgcttg tcccagagct gcagctgctt caggtacttc tcggcgcgct gccgcgcgac
     2221 cggacgcggg ataccgtaga accccgcctg attcaccacg atggtgaagg gtgtctcgaa
     2281 catgttgaag ttgatttcct gcggtaccac accgatgcac gccttggcgg cctcgagctc
     2341 acggtcgatg tcgtgcccga agacgctgat ctggccccca ctcttgttga cgagcgaggt
     2401 gacgatgccg atgagggtcg tcttgccggc gccgttcgga ccgagcaagg cgaagaaatc
     2461 gccctgctcg acgtcgaggt cgatgctttt cagagcctga acgccgttct tgtaggtttt
     2521 cgtgaggccg cgtacggaaa gtgcattcat ggtggaagga tcgggtttca agcggcggtt
     2581 cgcaaggcgc ggcgcgatgc gccgcctcag ccggcgaagt aacgggccac atactcctcg
     2641 tacgtcccct tgtcgccggc ctcgatcgct gcctggcggt cgagcgattc ctgcgcctgg
     2701 gcagcgaagt ccgcgagccg ctcctcattg ggcgggtaca gctcgaggaa gtacgccttg
     2761 tgggtcgccg acatccgcag cgccagatca aagaaggact caccggtttg ctgcagctcg
     2821 gccatcaggc gcgcggaggg cgtgagcgtc acgtcctcga gcttggccgc ctggacggca
     2881 agcgcctgcg agtacgggcg cgagggatcg ccctgatcga gcacctcgca gatgcccgtc
     2941 atggaatcga gcagctcctg cgcccagacg cgcatcggtg cggcacggcc ttcacgccat
     3001 aacgtcagcc ccggctcgcg accccgtctg gcgacggtca catggttctg atcgagtgcc
     3061 gcctgctctg cgtcatcgat cggcggactg gacttcatca ggcaaagcgc cagaaaggcc
     3121 tcgaggaagc gcagcttgtt ctgattcacg ccgaccggat cgaaggcgct cacatccagc
     3181 gcccgcacct cgacgtattc gaccccggct ctccgcagag ccttggtggg gcgctcgccg
     3241 gaacgcgcca cgcgcttcgg gcggatgaag ctgtagtact cattctcgat ctgcagcaca
     3301 ttcgcattga cctgttggta ctcgccacgg gcatcgcgga tacccagcgc ctcatagggc
     3361 ggatgcaccg tcgtgatcgc gtgtgtcaga tcgcgcacgt attcatcgag actgttgacc
     3421 gagacatgca ggccggcctg gttacgattt cggtagccca cgtcgctcat gcgcagactc
     3481 gtcgcataag gctcatacgc ggtgtttccc gtaagatcgg tcagctccag atgccggcct
     3541 ctgaggaagg acttgcacac gaccggtgag acgccgaaca gatacagcac gatccagccg
     3601 tagcgccgat agctgcgcag cagctcgaag tagctcgcgg agatgaagt
//
LOCUS       DASRHN010000412         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1310709, whole genome shotgun sequence.
ACCESSION   DASRHN010000412 DASRHN010000000
VERSION     DASRHN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310709"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFB37_16625"
     CDS             <1..401
                     /locus_tag="VFB37_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZO24121.1"
                     /translation="QLLDAAGVQVAVISGRRSRMVALRCRELGVRHVMQDAQDKVAAF
                     AKLRARLRVPAGECACVGDDVLDVPLMGKVGLAFAVADAHPAALRAAHVVTSLPGGYG
                     AVREVCDYLVDARQGKSRPTRSSRRKPARR"
     gene            398..958
                     /gene="lptC"
                     /locus_tag="VFB37_16630"
     CDS             398..958
                     /gene="lptC"
                     /locus_tag="VFB37_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZO24122.1"
                     /translation="MIFRIFATVVVLAIIAASIWFGGPQRGAIAPTPVEASSTDLGYS
                     ARRAVLIETGPDGLPMYTVHADVIRERSVNDVEFTQVQMSFRDQAGQIWTARADHGEL
                     GQQTGSVELTGDVHVDGLLPGSPQNADLSTEKLAVDSRADVISTDDPVTLDWGGRKLS
                     SHGMVAALAQRRLLLESAVHGIFSAP"
     gene            936..1595
                     /gene="lptA"
                     /locus_tag="VFB37_16635"
     CDS             936..1595
                     /gene="lptA"
                     /locus_tag="VFB37_16635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HZO24123.1"
                     /translation="MASFRHLSPFIALLLLGLPGLPATAQGAAARNILPSPSKDQRKL
                     PISLDAASSEVDYKTNTVVFKDIVISQGDTKVQADHARATGLDFDNSRWTFEGNVRIN
                     GEQHGSLHSDQATVDFRNNRIARATIDGSPAEFEQKRADSDQVARGHAKEIVYDVNDA
                     TVRLSDDAWLSDGRTETSAPLLVYDIRKQQLQAASKPETDQRVHIKIDPRSKDEIHPK
                     P"
     gene            1592..2317
                     /gene="lptB"
                     /locus_tag="VFB37_16640"
     CDS             1592..2317
                     /gene="lptB"
                     /locus_tag="VFB37_16640"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZO24124.1"
                     /translation="MTSLRATGLAKSYKSRQVVQNLSVEVANGEVVGLLGPNGAGKTT
                     AFYMIVGLVPCDAGRIHLGEIDLTTLPMHRRAQLGLGYLPQEASVFRKLSVENNVLAI
                     LETRADLDRGAREQRLEELLEELRIGHVRRSLGLSLSGGERRRVEIARALAAEPRFML
                     LDEPFAGVDPLSVLDIQRIIRELTSRDIGVLITDHNVRETLGVCSRAYIVNQGTVIAA
                     GTAEEILANPQVREVYLGESFRL"
     gene            2398..>3097
                     /locus_tag="VFB37_16645"
     CDS             2398..>3097
                     /locus_tag="VFB37_16645"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24125.1"
                     /translation="MLKPTLQLKLGQQLTMTPQLQQAIRLLQLPALELQAHIRELLET
                     NVMLEPTEDTDGTSTLEAIEEPGTHAEPAERPRESNVEVVDEGWGEHNVGPAEGPWTS
                     DDEERQQEFADASGQSLQEHLLWQLELAKLEPREFAIARAIIDSISDDGYLIDPLEEI
                     ANTLRPEVECDAAEVEAVLAGVQALDPPGIGARSVGECIQLQLRQLDPATPGFNTAIQ
                     IAQHHLELVAERELS"
BASE COUNT          554 a          983 c         1030 g          530 t
ORIGIN      
        1 ttcagctgct ggatgcggcg ggtgtgcagg tcgcggtgat ctccgggcgg cgctcgagaa
       61 tggttgcgct tcgttgtcgc gagctgggcg ttcggcacgt gatgcaggat gcgcaggaca
      121 aggtggccgc attcgcgaag ctgcgcgcgc gcctgcgggt gcccgccggt gaatgcgcct
      181 gtgtcgggga cgatgtgttg gatgttcctt taatgggcaa agtggggctt gcattcgcgg
      241 tggcggatgc gcaccccgcc gcgctccgcg ccgcgcacgt cgtgacctcc ctgcccgggg
      301 gctatggggc cgtcagggag gtgtgcgact atctggtgga cgcgcgtcaa ggcaaatccc
      361 ggcccacgag gtcctccagg cgcaagccag cacgccgatg atctttcgaa tctttgcgac
      421 tgtcgtcgtc cttgccatca tcgcggcttc gatatggttc ggcgggccgc agcggggggc
      481 tatcgcgccg acaccggtgg aggcttccag cacggatctg ggctactccg cccgccgagc
      541 ggtgctgatc gagaccggtc ccgacggtct gcctatgtac actgtgcacg cggatgtcat
      601 tcgcgagcgc tcggtgaacg acgtggaatt tacgcaggtg cagatgtcct tccgtgatca
      661 ggcgggacag atctggaccg cccgcgccga tcacggggag ctcgggcagc aaaccggcag
      721 tgtcgagctg accggggacg tgcacgtcga tgggctgctg ccgggttcgc cgcagaacgc
      781 ggacctctcg accgagaagc tggccgtgga cagccgcgcc gatgtcatca gcaccgatga
      841 ccccgtgacg ctcgactggg gcgggcgcaa gctcagctcg cacggcatgg tcgcggccct
      901 cgcgcaacgc cgtctgcttc tggaatcggc tgttcatggc atcttttcgg caccttagtc
      961 cgttcatcgc gctgctgctg ctggggctgc caggactacc agcgactgcg cagggcgccg
     1021 cggcgcgcaa catcctgccg agcccgtcca aggatcagcg caagctgccg atctctctcg
     1081 atgcggcctc ctccgaggtg gactacaaga cgaacaccgt ggtcttcaag gacatcgtga
     1141 tctcgcaggg cgatacgaaa gtgcaggccg accatgcccg ggccaccggg ctcgacttcg
     1201 acaacagtcg ctggacattc gagggcaacg tgcgcatcaa cggtgagcag cacggcagcc
     1261 tgcattcgga tcaagccacc gtggattttc gcaacaaccg gatcgcccgg gcgacgatcg
     1321 atggcagccc ggcggaattc gagcagaagc gggcggattc cgaccaggtc gctcgcggcc
     1381 atgcgaagga gatcgtttac gacgtgaacg atgccaccgt gcgcctctcg gacgatgcct
     1441 ggctttcgga tgggcgcacg gaaacgagtg cccccctgct ggtctacgac atccgcaagc
     1501 agcagctgca ggccgcttcc aagccggaga ccgatcagcg cgtgcacatc aagatcgatc
     1561 cgcgctcgaa ggatgagatc caccccaagc catgacctca cttcgagcca cgggcctggc
     1621 aaagagctat aaatcgaggc aggtcgtcca gaacttatcc gtcgaagtcg ccaatggcga
     1681 ggtcgtgggc ctgctcggac ccaacggcgc gggcaagacg accgccttct acatgatcgt
     1741 cggactcgtg ccctgcgatg ccggtcggat ccacctgggt gagatcgacc tgacgacctt
     1801 gccgatgcac cggcgcgccc aactcggtct gggctatctg ccgcaggaag cctccgtctt
     1861 ccgcaagctt tcggtggaga acaacgtgct tgcgattctc gagacgcggg cggacctgga
     1921 tcgcggggct cgtgagcagc ggctcgagga gttgctcgag gagctgcgga tcggacacgt
     1981 gcgacgcagc ctcgggctgt cgctgtccgg cggtgagcgg cggcgtgtgg agatcgcccg
     2041 ggcgctggct gccgagccgc gcttcatgct cctggatgag ccctttgcag gcgtcgatcc
     2101 gctttcggtg ctcgatatcc agcggatcat ccgcgagctc accagccgcg acatcggggt
     2161 gctcattacg gaccacaacg tcagggagac attgggggtc tgcagccggg cctacatcgt
     2221 caatcagggc acggtaatcg ctgcgggaac tgcggaagaa atccttgcca acccccaagt
     2281 ccgtgaggtg tacttgggtg agtccttccg gctgtagaac cgtctagcgc ttgagcggca
     2341 acccgagaat tttcagatac ttatgatgga aaggcccctg ccgcctgccg agcagccatg
     2401 ctgaagccga ccctgcagct caagctgggt cagcagttga ccatgacccc ccagctgcag
     2461 caggcgattc gactcctgca gctgcccgcc ctcgagctcc aggcccacat ccgcgagctt
     2521 ctcgaaacga acgtgatgct ggagcccacg gaggataccg atgggacgag caccctcgag
     2581 gcgatcgagg aaccggggac ccatgccgag ccagccgaac gtccccgcga gagcaatgtc
     2641 gaggtcgtgg acgagggatg gggtgaacat aatgtggggc cggccgaggg gccctggacc
     2701 agcgatgacg aggaacggca gcaggagttc gccgatgcct ccggacagtc gctgcaggaa
     2761 cacctgctct ggcaactcga gctcgcgaag ctcgagccgc gcgagttcgc cattgcccgc
     2821 gccatcatcg attcgatcag tgacgacggc tacctgatcg atccgctgga ggagatcgcc
     2881 aacaccctgc ggcccgaggt cgagtgcgat gcggccgagg tcgaggcagt cctggccggg
     2941 gtccaggcgc tcgatccgcc cgggatcggc gcccgctcgg tcggagaatg catccagttg
     3001 cagcttcgcc agctcgaccc ggccacaccc ggattcaaca ccgcgatcca gattgcccag
     3061 catcatctgg agctggtggc ggagcgtgag ctttccc
//
LOCUS       DASRHN010000413        10752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_153332, whole genome shotgun sequence.
ACCESSION   DASRHN010000413 DASRHN010000000
VERSION     DASRHN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153332"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFB37_16650"
     CDS             <1..690
                     /locus_tag="VFB37_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO24126.1"
                     /translation="FLCSPGNPTGAVLGEDFLRRALELAERFDFIVAADECYTEIYLD
                     ERAPPPGLLPTAFAAGRQRFERCIVFHSLSKRSSVPGLRSGFVAGDPAVMARYQLYRT
                     YHGCAMPVPTQLASIVAWDEEQHVAANRHLYQQKFDRVLPILAQIWRVERPAGAFYLW
                     PDIGGDDEEFTRELFANTHISVLPGSYLARAHGSRPNPGVGRVRISLVPAVEQCVEAA
                     ERIRDFVASRR"
     gene            694..1521
                     /gene="dapD"
                     /locus_tag="VFB37_16655"
     CDS             694..1521
                     /gene="dapD"
                     /locus_tag="VFB37_16655"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:TIGR00965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZO24127.1"
                     /translation="MSTRALQNTIEEGFERRAQLTPGSLPFGLASALEEAIELLDSGR
                     ARVAEQRDGTWVVNQWLKKAVLLYFRTRDNVVLDAGFTRFYDKVPLKYAATAEADFKS
                     AGARVVPHAVVRKGAYIAPDVVLMPSYVNIGAYVGAGTMVDTWATVGSCAQIGRNVHL
                     SGGVGIGGVLEPLQAAPTIIEDECFIGARSEIVEGVVVEAGSVISMGVFIGQSTRIFD
                     RESGAVLQGRVPAGSVVVPGSLPSNDGRYSLSCAIIVKRVDAQTRAKTSLNELLRTT"
     gene            complement(1627..3369)
                     /locus_tag="VFB37_16660"
     CDS             complement(1627..3369)
                     /locus_tag="VFB37_16660"
                     /inference="COORDINATES: protein motif:HMM:NF002505.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HZO24128.1"
                     /translation="MTELARLPSLLTRLRLILFGHEDLASIVFWAALVGFCGALVSVL
                     FREGIHLLELLFTGERLGLVHAASDLVWWHRAIVPLFGGLLAGLVLHLTGRALTSPRA
                     VDYMEAVLVGDGRISSRATLSNSFSSLLTISSGGSIGREGPMVQLSAMLGSKLGLLAH
                     APIPRLRLMVACGGAAGIAAAYNAPISGALFVSEIVLGSMAMESFGPLVVASVTSSAT
                     IHQFLGYGPVFDVPHVVFVSNWELIFFVILGVALGHLAPPFLALLDFAKSRFVRLELP
                     RYWQLGLGGLIVGILSIWFPQVWGNGYSVVGNILHADLFGWWLLAVLAAKVLATSATV
                     GSGAVGGVFTPTLFIGCAVGALFGGVVHSLLPGITSVPAAYALIGMGGFLAATTHAPL
                     TSILMLFEMTADYQIVLPLMLACVSAHYAAKVYRRGESVYHASLNRALATSGDDWRVR
                     TVESLVEPATAVVPPTMTVKEMLEQLPGRPLERIYVIDAGEIVAWVSPREVIERIGRG
                     ELASDVLVSAVANPVTFTLTPDMSLCSALDGFLREQATVLPVTPGQWRNTLLGQVSRH
                     DLLLAIQDRLTYPK"
     gene            complement(3435..5000)
                     /locus_tag="VFB37_16665"
     CDS             complement(3435..5000)
                     /locus_tag="VFB37_16665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO24129.1"
                     /translation="MRRRLAHCARGLWGASLTLLSACAVGPNYHRPDEHVPDHYAAMT
                     APAIPDKAGETPVAQPVDVDMARWWKALHDAELESLVDRAIRANPDLQIALDRLQAAR
                     TYEIALIGSVLPDAQASAGAGRGTGSDLTRGRTAQSLISADNSSGLQHINEVAGFDSV
                     WELDIFGKYRREMQAARYDVKASLAQRNAVLVSVVADVVRAYIDLRGLQMRASVLHAS
                     IETLQESLRIVKIRYERGITNELDVTLATRELATLQAQVAPVDAQVKAAQYTIATLLG
                     EYPEALVAELTTPTLIPSAPAAVHSELPVQLLRRRPEIMEAEGQLAAATARIGVATAN
                     LFPQIAITGAIGFQRQGLGTTPLLGEHIWSLGPGAVWPLLDFGLLDSQVETADLQTRA
                     LLVNYKRTIQNAVKDVDTAWSAYAAQQVRLGRLGEALVASQRAVTLATERYERGLTDF
                     LNVIDAQRQNYDIEEQYTDAQVGVAEEFVALYRSFGGGWEDYQSLPPAHIPEPAIIAA
                     FHRVLAHNDPLKQ"
     gene            complement(4997..6340)
                     /locus_tag="VFB37_16670"
     CDS             complement(4997..6340)
                     /locus_tag="VFB37_16670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO24130.1"
                     /translation="MQPPDDPTVQPGQEDSQEARRTDFADDIAEHESGAVSMQFDPRT
                     GQRLKRGAVIFAIVLAVFFIAVWIDRYVKERGVRSAAQQASSAVPSVEVIRAKPVGAV
                     QRFSLPGQTAAWHSSTIYARVNGYVGKWFTDIGDKVQKGEVLALIETPELDAQLTAAR
                     AQLKAAEAQVQARTSEMQFAKSTYDRWRDSPKGVVSEQEREEKRADYEGAVARLKSAQ
                     ADVALDQARVDQYMALAKFKQVAAPFDGVITDRYIDIGNLVTAGSTSATTPLYVMTQN
                     DPMRVFADVPQSASAELMQGHVPVEIQTGGSSPGHPYVGKVARTSEAMNPQARTLRVE
                     VDVPNAEQGLVPGMYVNVVFDLQPKGLVQVPAAALIFRSSGPEVARIDPSGRVRFRGV
                     SIARDDGNIVELGSGVAPGDELVLNASSQISEGEIVKASRQEPTGATPASATARR"
     gene            complement(6327..9536)
                     /locus_tag="VFB37_16675"
     CDS             complement(6327..9536)
                     /locus_tag="VFB37_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO24131.1"
                     /translation="MNLVQYVLRRPYTVVASLILVGLLGIGEALRMPMDIFPEIDIPV
                     VSVVWTYAGMNAQDIQNRILTFHERQMASLVDDISRIEATSYQGVGVERVYLHEGADV
                     TRAISQLASSALLVLKYMPPNITPPLVIRYGATDVPIIQLSLSSDSLPDTKLNDLGQN
                     IIRPALAVVHGAEVPYPYGGKPRVIMADLDERALQSRGLTPADVSEALQKQNVILPSG
                     DVKIGSRDYMLTMNNSPDVIEAINSFPIKEVDGRVVFMRDVAHVHDGFQVQTNAVSVD
                     GVPGALMTIRKTGGVSTLAVIDGVRAALPDIKHMLPPGVSIKPIFDQSVFVKAALNSV
                     LMGGCMAAGLTALMILLFLGNWRLTLIILASIPLSILTAVLFMGAGGETLNTMTLGGF
                     ALAVGILVDNGTVVIENIERHAGLHEHLSDAIIHGAREVGVPTMLSTLCICIVFVPVF
                     LLQGTARYLFSPLSVSVIVSLLASLVLSFTMVPVLFKYLMGTRAHGMQPAQARMHDSA
                     SGLANAFWRIHVGFERGFQQFREQYRNALAWSLSQAKITVLAFAALMVVSLLLFPELG
                     RDFFPQVDAGQMRLHVRAPPGTRLEQTEVDFSHVEKAIRQLLGAGQIDVILDNIGLPY
                     SGINISLSDSATVGPMDGEILIALGKHHTPTAELTAMLRRELPRRFPGLQFFFQPADI
                     VDQVLNFGQPAPIDVRVSASDTRSAYALASRLSHLIAGVPGVVDAHVFQVPDAPALSL
                     DVDRAFATEVGMTQRDAANNVLVATNSSAQSSPNFWVDPRNGVSYPLVVQQPTYSFGS
                     AQDLQTMPVTTPTHGADQGQLLMNLTKLGRESLPMVTSQLNIRPVFDVHADVQGRDLY
                     STSQDIDRVIQANQPPPAKAMHVTLSGQVETMRESYSGLFSGIALAVVLIYLFLVINF
                     QSWIDPLIVLMAVPFALGGVMWMLYLSQTHLSVPALMGTLMCIGLTTANSILVVTFAN
                     QRMELGDSPLVAAATAGYTRLRPVLMTAGAMILGMVPMALGVGEGGEQNAPLARAVVG
                     GLLFATFATLVFVPTMYALLRRRPAPAGVDLARGANATA"
     gene            complement(9538..>10752)
                     /locus_tag="VFB37_16680"
     CDS             complement(9538..>10752)
                     /locus_tag="VFB37_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO24132.1"
                     /translation="PEANDRFMRITRTDGRVVYASGRPQDNTFDPARIPPPPLQRQGS
                     FVRRQQLDNGSLLIGAVSHAGNPGYIVEVGVSAQHTDATLRQVLVLLLIGLPIGVCVA
                     VGGGFILVRRALKPVDDIAHKAAEITQLNLSERLPVMHTGDELERLSISLNHMISRLE
                     DAIQTSKRFVADASHELRTPLTVLRGELEGLAQDPALKAQTRESLGSLLEEVDRLAEI
                     VESLLALSRLDAGEANTEWVRFDLAELAASTAEQMSLLAEDKRITVVCESSTEVTVEG
                     DRARMKQVIVNLLDNAIKYTPHGGRVLLRITRDATRAILEVVDNGVGIPAEALPHMFK
                     RFFRVDDSRARPQGGAGLGLSIVKSICAAHGAEVDVTSTPGKGSSFRIVQPLAEAAAG
                     PVFIAGHPVRRS"
BASE COUNT         1967 a         3513 c         3487 g         1785 t
ORIGIN      
        1 ttcctgtgca gccccggcaa tccaacaggc gccgtcctcg gggaggactt tctgcggcgc
       61 gctctggagc tggccgagcg attcgatttc atcgtcgcgg ccgatgagtg ctacacggaa
      121 atctatctgg atgagcgcgc cccgccgcca gggctgctgc caaccgcgtt cgccgccggc
      181 cgccagcgct tcgagcggtg catcgtgttt cacagtctct ccaaacgctc cagcgtgccg
      241 ggtttgcgct ccgggttcgt cgcaggtgac ccggcggtga tggcccgcta ccagctgtac
      301 cggacctacc acggctgcgc gatgccggtc cccacgcagc tcgcgagcat cgtcgcctgg
      361 gatgaggagc agcacgtcgc agccaaccgg catctgtatc agcagaaatt cgatcgcgtg
      421 ctgccgatcc tcgcgcagat ctggcgagtc gagcgcccgg caggcgcctt ctatctgtgg
      481 cccgacatcg gcggggatga cgaggagttc acgcgcgagc tctttgccaa cacgcacatc
      541 agcgtgctgc ctggcagcta tctggcccga gcacatggca gccggcccaa ccctggcgtg
      601 ggccgtgtac gcatctcgct ggtgcccgcg gtcgagcagt gcgtcgaggc ggccgagcgc
      661 atccgtgatt ttgttgcgag ccgacggtaa agcatgagca cgcgagccct gcagaacacg
      721 atcgaggagg gtttcgagcg gcgcgcgcag ctcacccccg gctccctgcc ctttggactg
      781 gcctctgcgc tggaagaggc cattgagctg ctggatagcg ggcgcgcgcg ggtcgccgag
      841 cagcgcgacg gcacctgggt cgtcaaccag tggctgaaga aggcggtgct cctgtatttc
      901 cgcacccggg acaatgttgt cctggatgcc ggcttcaccc gcttttacga caaggtacca
      961 ctcaaatacg cagccacggc cgaggcggat ttcaaatcgg cgggtgctcg ggtggtccca
     1021 cacgcggtgg ttcgcaaggg cgcatacatc gcaccggatg tcgtgctgat gccgagctac
     1081 gtgaatatcg gtgcatacgt cggcgcaggc accatggtcg atacgtgggc caccgtcggc
     1141 tcctgcgcgc agatcggacg caacgtgcac ctctcaggag gggttggtat cgggggcgtg
     1201 ctcgaaccgc tgcaggccgc acccaccatc atcgaggacg agtgcttcat cggcgcgcgt
     1261 tcggaaattg tcgaaggtgt ggtggtcgag gcaggctcgg tcatctcgat gggcgtattc
     1321 atcggtcaaa gcacgcgcat cttcgaccgt gagagcggcg cggtgcttca gggtcgcgtg
     1381 ccggccggct cggtggtcgt gcccggctcg cttccctcca acgatggccg ctatagcttg
     1441 tcctgcgcca tcatcgtcaa gcgcgtggac gcccaaacac gcgccaagac ctccctcaac
     1501 gagctgcttc gcaccacata aactcgcaca gcagcgcgtg aggtatccgt atggggggag
     1561 atccccttgc atctaagcat tctcccgggg accgaccccc atacgatatc tcacgcgctt
     1621 gctcacctac ttcgggtagg tcaatcggtc ctgaatcgcg aggagcaggt cgtgccgcga
     1681 gacctgtccg agcagggtat tgcgccattg cccgggagtg acgggcagca cggtcgcctg
     1741 ctcgcgcagg aagccatcca gggccgagca taacgacata tccggtgtca gggtgaacgt
     1801 gacaggattg gctacggccg ataccaacac gtcggacgca agctcccccc gcccgattct
     1861 ctcgatcact tcacgcggac tgacccacgc aacgatctct ccggcatcga tgacatagat
     1921 ccgctccagc ggccggcccg gcaattgctc gagcatctcc ttcacagtca tcgtcggtgg
     1981 gacgacggca gtggcgggct ccaccagaga ctcgaccgtg cggactcgcc agtcatcgcc
     2041 cgaggtcgcg agcgcccggt tgagggaggc gtgatagacc gactccccgc ggcgataaac
     2101 cttggccgcg tagtgagcgg acacgcaggc aagcatcaac ggcagcacga tctgatagtc
     2161 ggccgtcatc tcgaaaagca tgaggatcga agtcaatggc gcatgcgtcg tggcggcgag
     2221 gaacccgccc atgccaatga gcgcataagc ggcgggcacg gaggtgatcc cgggcagcag
     2281 gctgtggacc acgccgccga agagagcgcc caccgcacag ccgatgaaca gcgtgggcgt
     2341 aaacacgccg ccgaccgcac ctgagcccac ggtggccgag gtcgccagca ccttggcggc
     2401 cagtactgcc agcagccacc aaccgaacag atccgcgtgc agtatgttgc cgacgacgct
     2461 gtagccgttg ccccagactt gcgggaacca gatcgagagg atcccgacga tcaggcctcc
     2521 gagcccgagc tgccagtacc gcggcagctc gaggcgcacg aagcgcgact tggcgaaatc
     2581 gagcagcgcg aggaacggcg gcgcgagatg acccagagcc acgccgagga tcacgaagaa
     2641 gatcagctcc cagttcgaga cgaacaccac gtggggtacg tcgaacaccg gcccgtatcc
     2701 gaggaactgg tggatcgtgg cgctcgaggt cacggaggcg accacgagcg gcccgaagct
     2761 ctccatggcc atcgagccca gcacgatttc cgacacgaac agtgcgccgg agatgggcgc
     2821 gttgtacgca gcggcaatac ctgccgcgcc accgcaggcc accatcaggc gcaggcgcgg
     2881 aatgggcgcg tgcgccagta ggccgagctt ggatcccagc atcgcggaga gctgcaccat
     2941 cgggccttcc cggccgatcg agccgccgga ggagatggtg aggagcgagg aaaagctgtt
     3001 ggaaagggtt gcccgtgagc tgattctgcc atcaccgacc agcaccgcct ccatgtaatc
     3061 cacggctcga ggagaagtca gcgcgcggcc cgtgagatgc aacacgaggc cggccagcag
     3121 tccgccgaac agcggcacga tggcgcggtg ccaccagacc aggtccgacg ccgcatgcac
     3181 cagccccagt ctttcgcccg tgaaaagcaa ctcgaggaga tgaatgccct cacggaacag
     3241 cacgctcacc agggcgccgc agaagcccac cagagcggcc cagaaaacga tgctggccag
     3301 atcctcgtgg ccgaacagga tcagccgcag tcgcgtcagc agcgacggca gccgtgcgag
     3361 ttctgtcacg ggagtctgca ttcgccgtca gcgtaaccgg gcgcaggtcg cgattgccaa
     3421 taccttggcc gggctcactg tttcagcgga tcgttgtgcg ccagcacacg gtggaaggcg
     3481 gcgatgatcg caggctccgg gatgtgggcg ggcggcagcg actggtagtc ctcccatccg
     3541 ccgccgaagc tgcggtacag cgcgacgaac tcttccgcca ctccgacctg cgcatcggtg
     3601 tattgctctt ctatgtcgta gttctgccgc tgcgcatcga tcacattcag gaagtccgtg
     3661 agcccccgtt cgtagcgctc ggtcgcaagc gtcacggcgc gctggctggc cacgagggcc
     3721 tcgccgagcc tccccagccg aacctgctgg gcggcgtagg cgctccaggc cgtatccaca
     3781 tccttgacgg cgttctggat cgtgcgcttg taattgacca gcagcgcacg cgtctgaaga
     3841 tcggcggtct cgacctggga atccagcagc ccgaaatcca gcaacggcca gaccgcgccc
     3901 ggacccagcg accagatgtg ctcgcccagg agcggggtcg ttcccaggcc ctggcgctga
     3961 aatccaatcg ctcccgtgat cgcgatctgg gggaacaggt tcgccgtggc gacgccgatg
     4021 cgcgcggtag cggcggcgag ctggccttcc gcctccatga tctccggccg tctgcgcagc
     4081 agctgcaccg gcagctccga atgcacggcc gccggtgccg aggggatcaa cgtcggggtc
     4141 gtcagctccg cgaccagagc ttcgggatat tcgccgagca aggtcgcgat cgtgtactgg
     4201 gccgctttga cctgggcatc gacgggcgcc acctgagcct gcagagtcgc cagctcacgg
     4261 gtggccagcg tcacatccag ctcgttggtg ataccgcgct cgtagcggat cttcacgatc
     4321 cgaagcgatt cctgcagcgt ctcgatcgat gcgtgcagca cggatgcgcg catctgcagc
     4381 ccgcgcagat cgatgtaggc tctgaccacg tctgctacga ccgagaccag tacggcgttg
     4441 cgttgagcaa ggctcgcctt gacgtcataa cgtgcagcct gcatctcccg gcggtacttg
     4501 ccgaagatat ccagctccca gaccgagtcg aagccggcca cctcgttgat gtgttgcaga
     4561 ccgctcgagt tatcggccga gatcagcgac tgtgcggtcc ggccgcgtgt cagatcactg
     4621 ccggtaccgc gtccagcgcc cgcactggct tgcgcatcgg gcagcacgct gccgatcagg
     4681 gcgatctcat aggttcgcgc ggcctggagc cgatccaggg cgatctgcag atccggattc
     4741 gcacggatgg cccgatcgac gagtgactcc agctccgcat cgtggagtgc cttccaccag
     4801 cgcgccatat cgacatccac aggctgagcg acaggggttt cgccggcttt atcagggatt
     4861 gcaggcgccg tcatggcggc atagtgatcg ggcacgtgct catcggggcg atggtaatta
     4921 ggacccacgg cacaggctga gagcagcgtg agcgaagctc cccagagccc gcgggcgcaa
     4981 tgtgcgagcc gccttctcac cggcgcgccg tcgcagatgc gggggtggcg ccggtcggct
     5041 cctgccggct cgccttgacg atctcgcctt ccgagatctg gctgctcgca ttgagcacga
     5101 gctcatctcc gggggcgacg ccggaaccga gctcgacgat gttgccgtca tcgcgcgcaa
     5161 tgctcacccc ccgaaaccgc acccgacccg acggatctat ccgtgctacc tcaggaccgc
     5221 tgctgcggaa tatgagggcc gccgcaggaa cctgcaccag gcccttcggc tgcaggtcga
     5281 agacgacgtt gacatacatt cccgggacca ggccctgctc agcattgggc acatccactt
     5341 ccactctcag cgtacgtgcc tgtgggttca tcgcctcgga ggttcgcgct accttcccca
     5401 cgtacggatg ccctggggag gagccgccgg tctggatctc tacgggcacg tgtccctgca
     5461 tgagctcggc ggaagcgctc tgcgggacat ccgcgaacac gcgcatcgga tcattctgag
     5521 tcatgacgta gagcggcgtc gtcgcactgg tgctgcctgc cgtcacgaga ttgccgatgt
     5581 cgatgtaccg atcggtgatc acaccatcga atggggcggc gacctgtttg aacttcgcca
     5641 gcgccatgta ctgatccacc cgcgcctgat cgagggccac atccgcctgc gccgatttca
     5701 gccgcgccac ggcgccctca taatcggcgc gcttctcctc gcgctcctgc tccgagacga
     5761 cgcctttcgg ggagtcgcgc cagcggtcgt aggtggattt ggcgaactgc atttcgctcg
     5821 tgcgcgcctg cacctgcgcc tcggcagcct tgagctgcgc gcgcgccgcc gtcaattgcg
     5881 catcgagttc gggggtctcg atgagcgcca gcacctctcc cttctgaacc ttgtcgccga
     5941 tgtcggtgaa ccactttccg acgtaaccgt tcacacgggc gtagatcgtg ctgctgtgcc
     6001 aggcggcagt ctgacccggc agactgaagc gctggacggc cccgacaggc tttgcccgga
     6061 tgacttccac actggggacc gcggagctgg cttgctgagc ggcactgcgg actccgcgct
     6121 ccttcacgta gcgatcgatc cagaccgcga tgaagaacac ggcgagcacg atcgcgaata
     6181 tgacggcacc gcgcttcaga cgctgtccgg tccggggatc gaactgcatc gagaccgcac
     6241 ccgactcgtg ctcggcgatg tcatcggcaa aatccgtccg gcgcgcttcc tgagagtcct
     6301 cctggccggg ttgaaccgtc gggtcatcag gcggttgcat tggcacctcg cgctaggtcg
     6361 actccagccg gagccgggcg ccgccgcagc agcgcgtaca tggtgggaac gaaaacgagc
     6421 gtcgcgaaag tcgcaaacag cagtccgccc acgacggcgc gcgcgagcgg cgcgttctgc
     6481 tcgccgccct cgccgactcc gagcgccatc gggaccattc cgagaatcat cgcgccggcg
     6541 gtcatcagca ccggccgcaa tcgggtatag ccggcggttg cggccgcgac caaaggacta
     6601 tcgccgagct ccatgcgctg gttggcgaag gtcacgacca gaatgctgtt ggcggtggtg
     6661 aggccgatac acatgagcgt gcccatcagg gccgggacgc tgaggtgcgt ctggctcagg
     6721 tacagcatcc acatcacccc accgagcgca aacggcacgg ccatgagcac gatgagagga
     6781 tcgatccagc tctggaagtt gatcaccagg aacaggtaga tgagcaccac ggcgagggcg
     6841 ataccggaga acagccccga atagctctcg cgcatcgtct cgacctggcc gctcagggtc
     6901 acgtgcatcg ccttggcggg cggtggctgg ttcgcctgga tgacccgatc gatgtcctgc
     6961 gaagtcgagt agagatcccg tccctggacg tccgcgtgca catcgaacac cgggcggatg
     7021 ttgagttgtg aggtgaccat cggcaggctc tcgcgcccga gcttggtgag gttcatgagc
     7081 agctgaccct gatccgcgcc gtgcgtcggg gtggtgaccg gcatggtctg caggtcctgc
     7141 gccgatccga agctgtaggt cggctgctgc accaccagcg gatagctcac gccgttgcgc
     7201 ggatcgaccc agaagttcgg cgatgactgc gcgctcgagt tggtggcgac cagcacattg
     7261 ttggctgcat cgcgctgcgt catgcccacc tcggtggcaa aggcccgatc gacatcgagg
     7321 ctcagcgcag gcgcatcggg aacctggaag acgtgcgcgt ccacgacgcc cggaaccccc
     7381 gcgatgagat ggctcaggcg cgaagcgagc gcgtaggcgc tccgggtgtc ggaggccgac
     7441 acccggacgt cgatgggcgc gggctgtccg aaattcagca cctggtcgac gatgtccgcc
     7501 ggctggaaga agaactgcaa acccgggaag cggcgcggca actcacgtcg cagcatggcg
     7561 gtgagctccg ccgtgggcgt atggtgcttg cccagcgcaa tgagaatctc cccgtccatc
     7621 ggaccgacgg tggccgaatc gctcagtgag atgttgattc cgctataggg caggccgatg
     7681 ttgtcgagga tcacatcgat ctggccggct ccgaggagct gtcggatcgc cttctcgacg
     7741 tgcgaaaaat cgacctcggt ctgctcgagg cgcgtgccgg gcggcgcacg cacgtgcagg
     7801 cgcatctgcc cggcatccac ctgcgggaag aaatcgcgcc ccagctccgg aaacagcagc
     7861 agcgagacca ccatcagggc ggcgaaggcg agcaccgtga tcttcgcctg cgagagcgac
     7921 caggcgagcg cgttgcggta ctgctcgcga aattgctgga agccgcgctc gaagcccacg
     7981 tgaatccgcc agaaagcatt cgcgagcccg gaggcgctgt catgcatcct cgcctgggcc
     8041 ggctgcatgc cgtgtgcacg cgtgcccatc aggtatttga acagcactgg caccatcgtg
     8101 aaggagagca cgaggctggc caggagcgag acgatcaccg agaccgacag tggggagaag
     8161 agataccggg cggtgccctg cagcaggaac accgggacga acacgatgca gatgcacagg
     8221 gtcgagagca tggtgggcac cccgacctcg cgcgccccat ggatgatggc atcgctcaga
     8281 tgctcatgca gccccgcgtg ccgctcgatg ttctcgatca cgaccgtgcc gttgtccacc
     8341 aggatgccca ccgccagggc aaatcccccc agggtcatgg tgttgagcgt ctcgccgcct
     8401 gcgcccatga agagcacggc ggtcaggatc gaaagcggga tcgatgccag gatgatgagg
     8461 gtcaggcgcc agttgcccag gaacagcagg atcatgaggg cggtcagccc cgccgccatg
     8521 cagcccccca tgaggacact gttcagcgcc gccttcacga acaccgactg atcaaagatc
     8581 ggcttgatcg agacgccggg cggcagcatg tgcttgatgt cgggcagcgc agcgcgaaca
     8641 ccatcgatca ccgccagcgt cgatacgcct cccgtcttcc tgatcgtcat cagcgcgccc
     8701 ggaacaccat ccaccgacac cgcgttggtt tgcacctgga atccgtcgtg cacatgcgcg
     8761 acatcgcgca tgaacaccac gcgcccatcg acctccttga ttggaaacga attgatcgcc
     8821 tcgatcacat ccgggctgtt gttcatggtg agcatgtagt cccggctgcc gatcttcaca
     8881 tcccccgagg gcaggatgac gttctgcttc tgcagcgcct cactcacatc cgcgggagtg
     8941 aggccacgcg attgcagcgc gcgctcatcg agatcggcca tgatgacccg cggcttgccg
     9001 ccatatggat aaggcacctc ggcgccatgc acgacggcca gagcaggccg gatgatgttc
     9061 tgccccagat cgttgagctt ggtgtcgggc agggaatcgc tcgacaggct gagctggatg
     9121 atcggcacgt cggtggcgcc ataacggatc acgagcggcg gggtgatgtt cggcggcatg
     9181 tacttgagga cgagcagcgc gctgctcgcg agctgggaga tggcgcgtgt cacgtcggcg
     9241 ccctcgtgca ggtacacgcg ctcaacgccg acaccctgat aactcgtggc ctcgatgcgc
     9301 gagatgtcat cgaccagtga ggccatctgc cgctcgtgga acgtcaggat gcgattctga
     9361 atgtcctgag cattcatccc ggcgtaggtc cacaccacgc tgaccaccgg aatgtcgatc
     9421 tccgggaaga tgtccatggg catgcgcagc gcttcaccga tgccgagcaa tccaaccagg
     9481 atgagactgg ccacgacggt ataggggcgc cggagcacat attgaacgag gttcatgtca
     9541 gctccgccgc accgggtgac ccgcgatgaa cacgggcccc gcggcggcct cggccagcgg
     9601 ctgcacgatg cggaaagagc tgcccttgcc cggcgtgctc gtcacatcga cctcggcgcc
     9661 gtgcgcagcg cagatcgact tgacgatgga cagaccgagc cccgccccac cctgcgggcg
     9721 cgcacgcgag tcatccacgc ggaagaatcg cttgaacatg tgcggcagcg cctcagctgg
     9781 aatgccgacg ccgttgtcca cgacctcgag gatggcgcgg gtggcatcgc gcgtgattct
     9841 cagcagcacg cggcccccgt gaggggtgta cttgatggca ttgtcgagca ggttcacgat
     9901 gacctgcttc atgcgggcgc gatcgccctc gaccgtgacc tcggtcgagg actcgcaaac
     9961 caccgtgatg cgcttgtctt cggccagcag gctcatctgt tcggccgtgc tggcggcgag
    10021 ctcggccaga tcgaaccgca cccactccgt gttggcctcg cccgcatcga ggcgcgaaag
    10081 ggccagcagg ctttcgacga tctccgcgag gcgatcgacc tcctcgagca ggctgccgag
    10141 ggattcacgc gtttgcgcct tgagggccgg atcctgggcg aggccctcca gctcgccgcg
    10201 cagtacggtg agtggggtgc gcagctcgtg cgaggcatcg gccacgaagc gcttggaggt
    10261 ctggatggca tcctccaggc ggctgatcat gtgattgagg gaaatcgaca ggcgctccag
    10321 ctcgtccccg gtatgcatca ccggcagacg ctcgctcaga ttgagctggg tgatctccgc
    10381 ggccttgtgc gcgatgtcat ccaccggttt cagggcacga cgcaccagga tgaacccgcc
    10441 accgacggcg acgcacaccc cgatcggcag cccgatgagg agcagcacca gtacctggcg
    10501 cagcgtggca tccgtgtgct gcgcggatac tcccacctcg acgatgtagc ccgggttgcc
    10561 ggcatgcgaa accgcaccga tcagcagcga gccgttgtcg agctgctgcc tgcgtacgaa
    10621 ggagccctgc ctctgaagtg ggggcggcgg aatgcgcgcc ggatcgaaag tgttgtcctg
    10681 cggtcttccg gaggcgtaca cgacgcggcc atcggtgcgc gtgatgcgca tgaagcggtc
    10741 gttcgcctcg gg
//
LOCUS       DASRHN010000414         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2298996, whole genome shotgun sequence.
ACCESSION   DASRHN010000414 DASRHN010000000
VERSION     DASRHN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298996"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="VFB37_16685"
     CDS             <1..1770
                     /locus_tag="VFB37_16685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZO24133.1"
                     /translation="DQLYSKDLPLLAAALGRPARVALLKGRGNYLCRQRLAEAGPQNA
                     ELWEPEPASRGMLARIQKWARTTRVGDLSELRGLSDSHPVWAQVTSSRENCLGRACPE
                     YARCHLVAARREALEADIVVVNHHLLLADLALKEDGFGDILGACDAVVLDEAHQIPDL
                     ATQFFGAHLSSRRIENLLKEIRLELASAEDNSGSLIETAALEVEQAVQRISACLPAAP
                     GRISWTEVDSTLSRAVMQLACAVQRLQSVLAPLAAESPLALIAGRTQDLFSSLERIAA
                     TDELEGARTAETLQRGFALSLMPFDISRRFQALLGARACAWIFTSATLSLGEDFSHFT
                     GRLGLDEIDTLKIDSPFDYERQCVLYLPAGMPEPASPGYVTAVIETALPLIEAARGGA
                     FILFTSHRALAQGAALVRASWSATMPFRLFVQGEAPRERLLQDFRADGNAVLLGTTSF
                     WEGVDVKGHALRLVIIEKLPFASPDDPLVRARIDHLQATGGNAFRDYQLPEAALALKQ
                     GVGRLIRSEEDFGAVVICDPRMVGRGYGRVFVSALPAMSVTRDSQEVVRFLRRYASEA
                     APAAEVGSSAMRQSLSASGEPAV"
     gene            1778..2485
                     /gene="tsaB"
                     /locus_tag="VFB37_16690"
     CDS             1778..2485
                     /gene="tsaB"
                     /locus_tag="VFB37_16690"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZO24134.1"
                     /translation="MRILALDTATEACSCALLMSDRLLRRDTELRKGHTEHILPMVDE
                     LLREAAITLPMLDAIAFGRGPGSFTGVRLAASVAQGLAFGAGRPVIGISDLRAVAQGV
                     AGNNSSATRVLVCNDARMREVYWACFEKGLDGLMQLVGEEHVGPPGSVRLPQAWGESS
                     ARSGSAVQGAGRGFGAYEELRSAWLERLAGIDTARLPAASEIALLAAAELRAGHAVSP
                     EEALPVYLRDDVAKPGS"
BASE COUNT          364 a          803 c          873 g          454 t
ORIGIN      
        1 gatcaacttt attcgaagga cctgcccctg ctcgcggccg cgctgggccg accggcgcgg
       61 gtggccctgc tcaagggccg gggcaactac ctgtgtcgac agcgccttgc cgaggccggt
      121 ccgcagaatg cggagctgtg ggagcccgag cctgcctcgc gcggcatgct cgcccggatt
      181 cagaaatggg cgcgcacgac ccgggtcggg gatctgtcgg agctgcgcgg gctttcggac
      241 tcgcaccccg tgtgggccca ggtcacttca tcgcgagaga actgcctggg gcgagcctgt
      301 cccgaatacg ctcgctgcca tctggtggcc gcgcgccgcg aggcgctcga ggccgacatc
      361 gtggtcgtca accatcacct gctcctggca gacctggcgc tcaaggagga cggcttcggg
      421 gacatcctcg gcgcttgcga tgccgtggtg ctcgatgagg cgcatcagat ccccgatctc
      481 gccacgcagt tcttcggcgc ccacctcagc agtcgtcgaa tcgagaatct gttgaaagag
      541 atccggctcg agctggcctc ggccgaggac aactccggat cgctcatcga gacggctgca
      601 cttgaggtcg agcaggccgt gcagcgcatc tcagcctgtc tgccggcagc tccggggcgc
      661 atctcctgga ccgaggtgga ttccacgctg agccgcgcgg tgatgcagct cgcgtgtgcc
      721 gtacagaggc tgcaatccgt tctggccccg ctcgctgccg agagtcctct cgccctcatc
      781 gccgggcgga ctcaggatct gttctccagc ctggagcgca tcgccgccac cgatgagctc
      841 gagggtgcgc gcacggcgga gaccctgcag cgcggctttg cactgagtct catgcccttc
      901 gacatctctc ggcggtttca ggctcttctc ggggctcggg cttgcgcatg gatcttcacc
      961 tcggccacgt tgtcactcgg cgaggatttc agccacttca ccggccgact cgggctcgat
     1021 gagatcgaca cgttgaagat cgacagcccg ttcgactatg agcggcaatg cgtgttgtat
     1081 ctgccggccg ggatgcccga gccagccagc cccggatatg tgactgcggt gatcgagacc
     1141 gcgctgccgc tgatcgaggc cgcgcgcggc ggcgccttca tcctcttcac cagtcaccgg
     1201 gctctcgcac agggtgcggc cctggtgcgg gcgagttggt ccgcaaccat gccatttcgc
     1261 ctcttcgtgc agggtgaggc cccgcgcgag cggctgcttc aggacttccg cgcggatggc
     1321 aatgccgtgc tgctgggcac gacgagtttc tgggagggcg tggatgtcaa gggacatgcc
     1381 ttgcgcctcg tcatcatcga gaagcttccg ttcgcctcac ctgacgatcc actcgtgcgc
     1441 gcccggatcg atcatctgca ggccaccggt ggtaatgcgt tccgcgatta ccagttaccc
     1501 gaggcggcac tcgctctgaa acagggcgtg gggcgcctga tccgcagcga ggaggacttc
     1561 ggtgcggtcg tgatctgtga tcctcgcatg gtcggtcgtg gctacgggcg cgtgtttgtt
     1621 tctgcgctgc ccgcaatgtc cgtaacacgg gactcacagg aagtcgtgcg tttcctgcgg
     1681 cgatacgcgt cggaggcagc cccggcggcc gaagtgggct cctctgccat gcggcagagc
     1741 ctgagcgcct ccggcgagcc cgcggtctga cttggggatg aggattctgg cgctcgacac
     1801 ggccaccgaa gcgtgctcgt gcgcgctcct gatgtcggac aggcttctgc gccgggacac
     1861 ggagctgcgc aaaggccaca ccgagcatat cctcccaatg gtggacgaat tgctccgcga
     1921 ggcggcgatc acgctgccga tgctcgacgc catcgcattc gggcgtgggc caggctcctt
     1981 taccggtgtc cgcctcgcgg caagtgttgc gcaggggctc gcctttgggg ccggtcggcc
     2041 ggtcatcggg atctcggact tgcgcgcggt cgcgcagggt gtggcgggga ataacagctc
     2101 cgcaacccgg gtcctcgtct gcaacgatgc ccggatgcgc gaggtgtact gggcgtgctt
     2161 cgagaaggga ctcgacgggc tgatgcagct ggtgggagag gagcacgtcg ggccgcccgg
     2221 tagtgtccgg ctcccgcagg cctggggcga gtcgagcgcg cggtccggct cagcagtcca
     2281 gggtgccggg cgcggcttcg gcgcttacga ggagctgcgg agcgcgtggc tggagcgact
     2341 ggccgggatc gatacggctc gtctgccggc ggcgagcgag atcgcgctgt tagccgcggc
     2401 ggagctgcgg gcgggacatg cggtctcgcc agaagaggcg ctcccggtgt atctgcggga
     2461 tgacgtggcg aagcccggtt catgaggctg acac
//
LOCUS       DASRHN010000415         8047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1477694, whole genome shotgun sequence.
ACCESSION   DASRHN010000415 DASRHN010000000
VERSION     DASRHN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477694"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFB37_16695"
     CDS             <1..392
                     /locus_tag="VFB37_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZO24135.1"
                     /translation="AIEGFVAEKTGLINQVGRDTLPQLLALLARSRVLLAPDSGPVHM
                     ATMVGTPVIGLYAATNPARSGPYLSRQWCVDAYGRAAQRFCGVPAEKLRWTRKIELPG
                     VMDLIEVSEVLSRLDGLLAGPSPSRSG"
     gene            447..839
                     /locus_tag="VFB37_16700"
     CDS             447..839
                     /locus_tag="VFB37_16700"
                     /inference="COORDINATES: protein motif:HMM:NF039364.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HZO24136.1"
                     /translation="MNDVLVPVSPGELLDKITILRIKAVRVTDPAKVANIRLELGLLE
                     DIWRDSGCAQRISPGEERALHEVNERLWDIEDRIRAKEAVQTFDREFIDLARAVYLAN
                     DERAAIKKRVNIQLGSRIVEEKSYKPYR"
     gene            884..1081
                     /locus_tag="VFB37_16705"
     CDS             884..1081
                     /locus_tag="VFB37_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HZO24137.1"
                     /translation="MAAMTKPLIQPNAQNQYDVSPEDLPLSCPMPQMFLWNSHPRVYL
                     PIQESGWAKCPYCGAEYTLKG"
     gene            complement(1088..4009)
                     /gene="glnE"
                     /locus_tag="VFB37_16710"
     CDS             complement(1088..4009)
                     /gene="glnE"
                     /locus_tag="VFB37_16710"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HZO24138.1"
                     /translation="MELSGQDPAALATLSSTSVELQASVHSAIEALLAAPRAASALQS
                     APAAIRSSLPLVFASSEFVAQACVRDAQLLPDLIAHGDLQRSLSATQMAARAPASGDN
                     TASDSQLLADLRTWRRRELTRIAWRDLAGWASTEETLADLTSFADAVLQAACDFASRP
                     LIARYGNPRSPAGEIQPLVVIGMGKLGGAELNFSSDVDLVLLFPEQGETDGQRPIANE
                     EFFTRLGQGLIRLLETPTHDGIVLRVDMRLRPFGDSGPLVASFASFEDYLPRHGRDWE
                     RYAYVKARPITSAERYAEIQASAIRPFVYRRYLDYGVFESLREMKLLIAREVQRRELA
                     EHVKLGPGGIREIEFIVQAFQLIRGGRDRRLQNTSLLKTLAVLGEERILPAATVAELR
                     SAYLYLRRLENRLQMLNDAQVHQLPEEPLSRERIALAMGAPDWAALLAELNAHRARVS
                     DYFRVVVLSGAEAEAGALRIDLGRFWDSQAETAALSESLDCAGIVHSEEATRILLELR
                     ASSLVRRLDEAGRKRLQALLPILLAEIASAHAATPDVQLEVLKRILRIIEAIGQRSAY
                     FSLLQESAGARARLVELCGHGDFLAQQIAAYPLLLDELLDERLLSHLPGRAELARELA
                     LRMQQAQDEEPEHQVEALKQFQRAAIFRIAVADLTGRLPVMQVSDRLTEIAELILERA
                     MDLGWAQMTAQFGVPHCGEGPQRRPVRIAAAGYGKLGGIELGYSSDLDLVFLHDSRGE
                     RQETAAPRPIDNQVFFVRIAQRIVHLLTTHSAAGRLYEVDVRLRPSGKGGLLVTSIRA
                     FLEYQRKEAWTWEHQALLHARAVAGAPELRAEFERVRLDVLCNEVRRDTLRSEVRAMR
                     ERMRKELSKASPAQFDIKQDPGGIADIEFLAQYWALSWAQEHPAVALFSDTIRQLESV
                     ASADLIPQTTVDVLTNAYRGYRARTHRLSLEARPPIIATEEFACERAAVTRIWNETME
                     P"
     gene            complement(4000..4989)
                     /locus_tag="VFB37_16715"
     CDS             complement(4000..4989)
                     /locus_tag="VFB37_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24139.1"
                     /translation="MTAIVKSAMQAANRGSFAGSWPGRIVAVTLTLCALQACSKMSAA
                     QHGEMDWARAALERNDQLQVVASDPQARTFTVRLKDSDELKVLSLDQLVAGPTVAANN
                     TPKQSEPTSAAADQATSPAAAGANAATAPQPAQPPAQGQTASNDQSAQPASGSENGAS
                     AAEPSGATPAPTAGPHAEPASQAKAGSVLASGPGYTIKASGSQPAPGRAVTGQTSSRG
                     LPLERLHDPIICQGSRLLHIDNRNLQFDGDAVTAEDGCELHITNSHIQAKGIGVLARA
                     ASVHIENSEIDGDGGSIDASDGAQVYAEASRFKGLTRHMEASAFHDLGGNVWN"
     gene            complement(5420..7057)
                     /gene="groL"
                     /locus_tag="VFB37_16720"
     CDS             complement(5420..7057)
                     /gene="groL"
                     /locus_tag="VFB37_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZO24140.1"
                     /translation="MAAKEVRFSDDARQRMFKGVNVLANAVKATLGPKGRNAVLEKSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASNTSDEAGDGTTTATVLAQAIIRE
                     GLKAVASGRNPMDIKRGIDKGVASAVEELRKLSKPCKDSKAIAQVGTISANADESIGK
                     TIADAMEKVGKEGVITVEEGSGLDNELEVVEGMQFDRGYLSPYFINNAQNQTTELEKP
                     LILLADKKISNIREMLPLLEGVAKAGRPLLVIAEDVEGEALATLVVNNIRGILKVAAV
                     KAPGFGDRRKAMLQDIAVLTGGTVISDEVGLTLEKASLNDLGDAKKIVVEKENTTIID
                     GAGKQSDIKARIESIRQQAEEATSDYDKEKLQERVAKLSGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALLRAQKGLDKLEASNEDQQVGVRILARSIEEP
                     LRQIVDNAGEDAAVILNRVKEGKGAFGYNASTGTFGDMLEEGILDPTKVTRLALQNAA
                     SVAGLLLTTEVMIAEAPKEEEHSHGGMPPGGMGGMDM"
     gene            complement(7074..7367)
                     /gene="groES"
                     /locus_tag="VFB37_16725"
     CDS             complement(7074..7367)
                     /gene="groES"
                     /locus_tag="VFB37_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009684394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZO24141.1"
                     /translation="MKIRPLHDRVIVKRLEEERTSPGGIVIPDTAAEKPVQGKIVAVG
                     KGKILEDGQVRPLDVKIGDKILFGKYSGTEVRVDGEELVVMREEDVMAVIEGK"
     gene            7820..>8047
                     /locus_tag="VFB37_16730"
     CDS             7820..>8047
                     /locus_tag="VFB37_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24142.1"
                     /translation="MHRASIGHNLCRLGKVLAFLAATALLPAPPLLAAPGGPPSALQA
                     AMSPGTSQSGDNFLPADEAFRFDAVAEGPDRI"
BASE COUNT         1353 a         2628 c         2549 g         1517 t
ORIGIN      
        1 cggccatcga agggttcgtg gcggagaaaa caggtctcat caatcaggtg ggtcgggata
       61 cgctccctca gttgctcgcg ttgctcgcgc gctcgcgggt gctgctggca cccgactcgg
      121 ggccggtgca tatggcgacc atggtgggca cgcccgtgat cgggctctat gccgccacca
      181 atccggctcg cagcgggccg tacctgtcgc ggcagtggtg cgtggatgcc tacggacggg
      241 cagcgcagcg gttctgcgga gttccagccg agaagctgcg ctggacccgc aagatcgagc
      301 tcccgggcgt catggatctc atcgaggtca gtgaggtgct cagcaggctc gatgggctgc
      361 ttgccgggcc ctcgccctcg cgcagcgggt gagtcctgta actctcccac catgcttcgc
      421 gccgcagcct gctgctacga taggccatga acgacgtcct ggtgccggtt tccccgggcg
      481 agctgctcga caagatcacc attctgcgca tcaaggccgt gcgggtcacc gatccggcca
      541 aggtcgccaa catccggctg gagcttgggt tgctggagga tatctggcgt gacagtggct
      601 gtgcgcagcg gatctccccg ggcgaggagc gggcgctgca cgaagtcaac gagcggctgt
      661 gggacatcga ggaccggatt cgggccaagg aggctgtgca gaccttcgac cgcgagttca
      721 tcgatctggc gcgcgcggtg tatctggcga acgatgagcg agctgcgatc aaaaaacgcg
      781 tcaacatcca gcttggctca cgcatcgtcg aggagaagtc gtacaagccc tatcgctgag
      841 cgccccgtat aatgcccacg acatctctcg aagtccgtat cccatggccg cgatgaccaa
      901 gcccctgatc cagccgaacg cgcagaatca gtacgatgtc tcccccgagg atctacccct
      961 atcctgtccg atgccgcaga tgttcctgtg gaactcgcac cccagggtct acctccccat
     1021 ccaggaaagc ggctgggcga aatgccccta ctgcggcgcg gagtacacgc tcaaaggctg
     1081 atcgccctca gggctccatg gtctcgttcc agatgcgcgt caccgcggcg cgctcgcaag
     1141 caaactcctc cgtggcgatg attggcggcc gtgcctccag ggagaggcgg tgcgtgcggg
     1201 cgcgatatcc ccgataggca ttggtcagca catccaccgt cgtctgcgga atgagatcgg
     1261 ccgatgccac cgactcgagc tggcggatcg tgtccgagaa caacgccacc gccggatgct
     1321 cctgggccca gctcagagcc cagtactgag ccaggaattc gatgtcggca atgccgccgg
     1381 gatcctgctt gatgtcgaac tgcgcggggg atgctttgga cagctccttg cgcatgcgct
     1441 cgcgcatcgc tcgaacctcg ctccggagcg tgtcacggcg cacctcgttg cagagcacat
     1501 ccagccggac gcgttcgaat tcagcacgca gttcgggcgc gcccgctaca gcgcgtgcgt
     1561 gcagcaaggc ctgatgctcc cacgtccagg cttccttgcg ttgatactcg agaaaggcgc
     1621 gaatactggt gacgagcagc cctcctttgc cgctcgggcg aagacggaca tcgacctcgt
     1681 acaggcgccc ggctgcggag tgcgttgtca gcagatgcac gatgcgttgc gcgatccgca
     1741 cgaagaacac ctgattgtcg atcgggcgtg gggccgcggt ttcctgccgc tctccccgcg
     1801 agtcgtgcag gaagacgagg tccagatcgg acgagtagcc gagctcgatg cccccgagtt
     1861 tgccgtagcc ggcggccgcg atgcgcaccg ggcggcgctg cggtccctcc ccgcagtgag
     1921 gcactccaaa ctgagcggtc atttgtgccc acccgagatc catcgcccgt tcgaggatca
     1981 gctccgcaat ctccgtcagg cggtcgctga cctgcatgac cggtagacgc ccagtcagat
     2041 ccgccacggc gatgcgaaag atggcggccc gctggaactg tttcagcgcc tcgacctgat
     2101 gctccggctc ctcgtcctga gcctgctgca ttcgcagcgc gagctcgcgt gccagctccg
     2161 cgcggccggg caagtgcgac agcagccgct catcgagcag ctcatccagc agcaacgggt
     2221 aggcagcaat ctgctgcgcg agaaagtcgc catgtccaca aagctcgacc agccgggcac
     2281 gcgcgcccgc actttcctgc aggagcgaga agtaggccga gcgctggccg atggcctcga
     2341 tgatccgcag tattcgcttc agcacctcga gctgcacgtc gggggtagcg gcgtgagcgc
     2401 tggcaatctc cgccagcagg atgggaagca gggcctgcag gcgcttgcgg cccgcctcat
     2461 ccagtcggcg cacgagcgag gaggctcgca gctcgagcag aatccgggtc gcctcctcgg
     2521 aatgcacgat gcctgcgcag tccagcgact cggagagtgc tgccgtctcc gcctggctat
     2581 cccagaagcg tcccagatcg atcctcaggg ccccggcttc ggcctcggcg ccactgagca
     2641 ccaccacgcg aaaataatcg ctcacccgcg cgcgatgagc attcaactcc gcaagcagtg
     2701 ccgcccagtc gggggcgccc atcgcaagcg cgatccgctc gcgcgacaga ggctcctccg
     2761 ggagctggtg cacctgcgcg tcattgagca tctgcagccg gttctcgagg cggcgcaagt
     2821 acagataggc agaacgcagc tctgccacgg tggccgcggg caggatccgc tcctcgccga
     2881 gaacggcaag cgtcttcagc agcgaggtgt tctgcagccg gcgatccctg ccgccgcgga
     2941 tgagctgaaa tgcctgcacg atgaactcga tctcgcggat gccgccaggg cccagtttca
     3001 cgtgctcggc cagctcacgc cgctgcacct cgcgggcgat gagcagcttc atctcgcgca
     3061 ggctttcaaa cacgccgtag tccagatagc ggcgatagac gaacggccgg atcgcggagg
     3121 cctggatctc ggcgtagcgc tcagctgagg tgatcgggcg agccttcacg taggcgtaac
     3181 gctcccagtc gcgcccatgt cgcggcaggt aatcctcgaa agaggcgaag ctcgcgacca
     3241 gcggtccgct gtccccgaac ggccgcagcc gcatatccac ccgcagcacg attccatcgt
     3301 gagtcggtgt ctccagcagg cggatgagtc cctgtcccaa acgcgtgaag aactcttcgt
     3361 tggcgatcgg ccgctggcca tcggtctctc cctgctcggg aaacaacagc accaggtcaa
     3421 cgtccgatga gaaattgagc tcagcgccgc cgagcttgcc catgccgatg accacgagcg
     3481 gctggatctc tccggctggc gatcgggggt tgccatagcg cgctatcagt gggcgcgagg
     3541 cgaaatcaca tgcggcctgc aggaccgcat ccgcgaacga ggtcagatca gccagtgtct
     3601 cctcggttga ggcccagccg gcgaggtcgc gccaggcgat ccgggtcagc tcgcgacggc
     3661 gccaggtgcg cagatccgca aggagttggg aatcggaagc cgtgttatcc ccgctcgcag
     3721 gtgcgcgagc ggccatctgc gttgccgaca gagatcgttg cagatcgccg tgtgcgatca
     3781 gatccggaag caactgcgca tcgcgcacgc acgcctgcgc cacgaactcg ctggaggcaa
     3841 acacgagcgg cagcgagctg cggatggcgg ccggtgcgct ctgcagagcc gaggctgcgc
     3901 gcggggctgc gagcagcgcc tcgattgcgc tgtggacgct ggcctgaagc tcgacgctgg
     3961 tggaggaaag agtggccagt gccgcggggt cttgtccgct cagttccaga cgtttccgcc
     4021 cagatcatgg aaggccgaag cctccatgtg gcgcgtcagg cccttgaacc tggaggcttc
     4081 ggcatacacc tgggccccgt ctgaggcatc gatcgagccg ccgtcaccgt cgatctcgct
     4141 gttctcgatg tgaacgctgg cggcgcgcgc cagcacgcct atcccttttg cctggatgtg
     4201 cgagttggtg atgtggagct cgcagccgtc ctcggccgtc accgcatccc catcgaactg
     4261 cagattccgg ttatcgatgt gcaacagacg cgagccctgg cagatgatcg gatcgtggag
     4321 ccgctcgagc ggcaggccac gggagctcgt ctggccggtc acggcacgcc cgggcgcggg
     4381 ctgcgagccg ctcgccttga tcgtgtagcc cggacccgat gccagaacgc tgcccgcctt
     4441 ggcttgtgag gcgggctcgg cgtgaggtcc ggcagtaggg gcaggggttg cgcccgatgg
     4501 ttctgccgca ctcgcgccat tctcgctccc ggaggcaggc tgtgcgctct ggtcattcga
     4561 tgctgtttgc ccctgcgcgg ggggctgcgc tggttgcggt gccgtagcgg cgttggcgcc
     4621 ggcagcagca ggggaagttg cttggtccgc agcggctgat gtcggctcag actgcttcgg
     4681 tgtgttattg gcagcgaccg tgggaccggc cacgagctga tcgagcgaca gcactttcag
     4741 ctcgtcggaa tccttcagcc gcacggtgaa ggttcgggcc tgcggatcgg aggcgactac
     4801 ctgaagctgg tcgttgcgct cgagcgccgc acgcgcccag tccatttcac catgctgcgc
     4861 ggcgctcatt ttcgagcaag cctggagcgc gcaaagcgtc agcgtcacgg cgacgattcg
     4921 cccgggccag gaccccgcga atgagcctcg attcgccgcc tgcattgccg atttaacaat
     4981 agccgtcatg gtgctctcct gcggcgccca caggccagac gggcgctgca gacaacatac
     5041 cacgacaaca acaattgttg agcaatagcg caatcaccgc gccgttatag gggtttcgac
     5101 gccggcggcc ggttgttgtt ctaccggaca ctgcacacgg tccgtgccaa agggctacgc
     5161 gcggtgatcg ttggaagtgg gcgtggtgcg cagcgaagtt ttttgcggag gcgcgcgccg
     5221 cacttcgcgt cagttctcat tctcgctatc caaaaaagaa ccccgccgag gcggggtttc
     5281 aatattgcgc ggaatagggg gtcttgttcc gcgtctttcg gggcgtgccg cgccaaggtg
     5341 gcgccgcaca atcccttaac cgttgagaat tatcgtatcg atgttgtgtt acagaatcga
     5401 tatcccttta gagggggatc tacatgtcca ttccgcccat gccgcccggc ggcatgccgc
     5461 catggctatg ctcctcttcc ttcggcgcct cggcaatcat cacctcggtt gtcagcaaca
     5521 ggccggccac ggaagccgcg ttctgcaatg cgagacgagt caccttggta ggatcgagga
     5581 tgccctcttc cagcatgtcg ccaaacgttc ccgttgaggc gttgtagccg aaagcaccct
     5641 tgccttcctt gacgcggttg agaatcacag cggcgtcctc gccggcgttg tccacgatct
     5701 ggcgcagtgg ctcctcgatg gaacgtgcca gaatgcgaac acccacctgc tggtcctcgt
     5761 tggaggcctc cagcttgtcg agtcccttct gcgcacgcag cagcgcaacg ccgccgcccg
     5821 gtaccacgcc ctcttcgact gccgcacgag tcgcgtgcag ggcatcttcg acgcgggcct
     5881 tcttctcctt catctccacc tcggtcgccg caccaacctt gatgactgcg acgccaccgg
     5941 agagcttggc aacacgctcc tgcagcttct ccttgtcgta gtcggaagtg gcctcctcgg
     6001 cctgctggcg aatcgactcg atgcgcgcct tgatgtcgga ctgtttgccg gcgccgtcga
     6061 tgatggtcgt gttctccttc tcgacgacga tcttcttggc atcgcccaga tcattgagcg
     6121 aggccttctc gagcgtcaga ccgacctcat cggagatcac cgtgccgccg gtgaggaccg
     6181 cgatgtcctg cagcatggcc ttgcgacgat cgccgaagcc gggggccttg acggccgcaa
     6241 ccttcaggat gccgcggatg ttgttgacca ccagagtggc gagagcttcg ccctcgacat
     6301 cctccgcgat cacgagcagc ggccggcccg ccttggcgac gccttcgagc agcggcagca
     6361 tctcgcggat gttggagatc ttcttgtcag cgagcaggat cagcggcttc tcgagctccg
     6421 tcgtctggtt ctgcgcgttg ttgatgaagt acggggagag gtagccgcgg tcgaactgca
     6481 tgccttcgac cacttccagc tcgttgtcga gcccggagcc ttcctcgacc gtgatgacgc
     6541 cttccttgcc gaccttctcc atcgcatccg caatggtctt gccgatcgac tcatcggcgt
     6601 tcgcggagat cgtgccgacc tgcgcgatgg ccttggaatc cttgcacggc ttggagagct
     6661 tgcgcagctc ctcgaccgcg ctcgcgacgc ccttgtcgat gccgcgcttg atgtccatcg
     6721 ggttgcgtcc ggaggcaacc gccttcaggc cctcgcggat gatcgcctga gcgagcaccg
     6781 tggcggtggt cgtgccatcg ccagcctcat cggaggtgtt ggacgcgact tccttcacca
     6841 tctgcgcgcc catgttctcg aacttgtcct taagctcgat ttccttcgcg accgatacgc
     6901 catccttggt gatggtcggg gcgccgaagc tcttctcgag cacggcgtta cggcctttcg
     6961 ggccgagtgt cgccttgacg gcgttggcga ggacattcac gcctttgaac attcgctggc
     7021 gtgcgtcgtc gctgaatcta acttctttgg ctgccattat cgtgatcctt cggttacttg
     7081 ccttcaatca cggccataac gtcttcctct cgcatgacga ccaactcctc gccgtcgacc
     7141 cggacttccg tgccgctgta cttgccgaag aggatcttgt cgccgatctt gacgtcgaga
     7201 ggacggacct ggccgtcttc gagaatcttg cctttgccga ccgctacgat ctttccctgc
     7261 acgggctttt cggccgcggt atcgggaatc acgatgccgc ccggagaggt tcgctcctct
     7321 tccaggcgct tcacgatgac gcggtcatga agaggacgaa tcttcatgga tgtcgctcct
     7381 ttgaaactgc tgaaataaat ggattttttt gacccccggg cagcctgtta gcactctgcc
     7441 tgggcggctg ctaatgatag tgagcgaaga tccaatttca agcctgggca caactttttt
     7501 ttgcctcgag tgggggtccc ggagccggca ggtcgcgcag gcggacctcc cagaggcgcc
     7561 ggcgcgccgg aaacgcgggt cgcgccccga gcctcaccga tgcgcggtcc tcgagcgggc
     7621 cgcccttgcg ggcggggagc ggctcaatca gccggctgag ctgaacccgt gggctccgaa
     7681 gctctccaat ccagcgcggc acctgaaagc gctggcaggt gaggctttta gcgtcgaaaa
     7741 tgcgggccaa gtcacttcat ggggccggat gagcggaaat catcgggcag cgatgtctat
     7801 ctcacctgag cgagcgccta tgcaccgcgc ttccattgga cataacctct gccgcctggg
     7861 aaaggtgctg gccttcctgg ctgccaccgc gctgctcccg gcgcccccgc ttctcgccgc
     7921 tcctggcggt cctccctcgg cattgcaggc cgccatgagc ccggggacga gccagtcggg
     7981 agataatttc ctgcctgcgg acgaggcctt ccgcttcgat gccgtggcgg agggcccgga
     8041 ccggatc
//
LOCUS       DASRHN010000416        13102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1802063, whole genome shotgun sequence.
ACCESSION   DASRHN010000416 DASRHN010000000
VERSION     DASRHN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802063"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /gene="dnaE"
                     /locus_tag="VFB37_16735"
     CDS             complement(<1..1867)
                     /gene="dnaE"
                     /locus_tag="VFB37_16735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZO24143.1"
                     /translation="MLTGFVHLRLHTEYSLIDSVVRVPELIDAVAAAGMPAVALTDQN
                     NLFAMVKFYRAALAKGVKPLIGVDLRVREPGERQQGFRLTLLCQSQTGYRNVTRLLSR
                     AYLEGQQRGVPWVDPHWLTQESVQGLLALSCATQGDVGRALVNAREREAEQALERWLA
                     IFGDRFYIELQRLGHPEEEGYIAAAVSLASRCSVPLVATNDVRFLRAEDFESHEARVC
                     IHDGALLADPGRQRRYTAQQYLRTPQEMASLFSDVPEALANTVAIAQRCSLVLKLGEA
                     RLPQYPVPAGLSTEQFVREEASRGLEARLAGPSAAPVELYRERLRLELDVICQMGFAG
                     YFLIVADFIRWARENHIPVGPGRGSGAGSLVAYSLGITDLDPIHHDLLFERFLNPERV
                     SMPDFDVDFCMDGRDRVIDYVAQKYGRERVSQIITYGTMAAKAVVRDVGRVLGMSYGY
                     VDRIAKLIPFELGITLEDALAKEPELKRLYDSEDEVRNLIDLARSLEGLTRNAGMHAG
                     GVVIAPSVLTDFAPLYCDEAAGHVVTQFDKDDVEAAGLVKFDFLGLRTLTVIHSAVKT
                     INGIRAQRGEPPLEIGALPMDDPRTFALLKACSTTAVFQLESRGMKDLVRRLQPDR"
     gene            complement(1873..2535)
                     /locus_tag="VFB37_16740"
     CDS             complement(1873..2535)
                     /locus_tag="VFB37_16740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZO24144.1"
                     /translation="MRAQRVKRYAMKLLRARSRCGPMRCAGVDEAGRGPLAGPVVAAA
                     VILDPRRRIRGLADSKALTPEERARLAPIIRARALAWSVAWSDRDEIDCLNILGATFL
                     AMRRALLRLPVCPTHVQVDGNLLPRLDDLHLGCTAEAIVEGDACVAAISAASILAKTC
                     RDAMMEQLDACYPGFDLAAHKGYCTPGHLRALACREPSPLHRRSFQPIREHLLVLSPD
                     LD"
     gene            complement(2558..3829)
                     /gene="lpxB"
                     /locus_tag="VFB37_16745"
     CDS             complement(2558..3829)
                     /gene="lpxB"
                     /locus_tag="VFB37_16745"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZO24145.1"
                     /translation="MSELGAPVAPRPGLAAALASGPAGGPQSGAAAGRPQGTTAAPIS
                     IGLVAGESSGDQLGAALISALKALVPQVSCFGVGGPKMAAAGFEAWASSEELAVMGFT
                     EPLRHLPRLLRLRSMLIERFGAARPDAFVGIDAPAFNLGLAKRLKRQGMRTVQYVSPQ
                     VWAWRQGRVRSIGAACDLVLCLLPFETEFYVRHGVRALFVGHPLADQIPLSPDRAAAR
                     AALALDAQRQLIALLPGSRLAEVERLGSDFAAAASWLRARRPEFQFVAPMASARVRAV
                     FERQLARTARRPEILLLDGHAHTALAAADAAIVASGTATLETLLSGRPMVVAYRVSAL
                     TALLLRTLGLVKVPYFSQPNLLIGRARVPEFFQEQVSAEALGAALLRELEDPAHVEEL
                     RREFRAVHEALRLGGAQRAAAAILECARERR"
     gene            complement(3826..4596)
                     /gene="lpxA"
                     /locus_tag="VFB37_16750"
     CDS             complement(3826..4596)
                     /gene="lpxA"
                     /locus_tag="VFB37_16750"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZO24146.1"
                     /translation="MIDAHALISPQAQLAEDVTVGPFSVIGPDVQIGPRTVIGPHVVL
                     NGPTRIGADNRIFQFASIGDAPQDKKYKGERTSLEIGDRNVFRESCTVNRGTTHDRGV
                     TRIGNDNLFMAYSHVAHDCLVGSNVVFANCAALGGHVEIGDWVILGGLTAVHQFAKIG
                     AHAFLAGGAIVTRDVPPYVMVAGNPAEPHAVNAEGLKRRGFDEEQIRNVREAYRILYR
                     SDLRLTEALEKLRPIAESRSEIRAFVDFIGTSTRSIVR"
     gene            complement(4593..5048)
                     /gene="fabZ"
                     /locus_tag="VFB37_16755"
     CDS             complement(4593..5048)
                     /gene="fabZ"
                     /locus_tag="VFB37_16755"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZO24147.1"
                     /translation="MTDPIRLDINEVVRLLPHRYPFLLVDRVLECHPGKSIRALKNVT
                     YNEPYFPGHFPGRPLMPGVIIVEALAQAAGILAFVSAGVVPNHQTKFYFVGIDKARFR
                     KPVEPGDQLILFAQLERALKGIWRFSTVATVGDVEVAHADMMVAPETGK"
     gene            complement(5041..6078)
                     /gene="lpxD"
                     /locus_tag="VFB37_16760"
     CDS             complement(5041..6078)
                     /gene="lpxD"
                     /locus_tag="VFB37_16760"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZO24148.1"
                     /translation="MAVTLGELAVRFGCELRGNPDMRVERVGTLVNADDLCVAFLAED
                     RNRRELAATRAAVVVLGAAVAQFCPTAALICDNPRATFARIAALLYPDAPAKPGIHPS
                     ATVAPDARVDSSAHVGPHAVIGDRAVIGSHVFVGAHSVLENDVVLEEYVRLVAKVTLC
                     RGVRVGARTLIQPGAVIGADGFGFAPDRGRWLKVPQVGSVSIGPDVEIGANTTIDRGA
                     IEDTVIEEGAKLDNLIQIGHNVRVGAHTVIAGCTGISGSTIIGRRCIIGGAVGIAGHL
                     TICDGVTITGFAMVSRSLTSPGVYSSGIPIEESKVWRRQVARYRKLETYEKRLKQLER
                     AAGAAQDEGDD"
     gene            complement(6124..6693)
                     /locus_tag="VFB37_16765"
     CDS             complement(6124..6693)
                     /locus_tag="VFB37_16765"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZO24149.1"
                     /translation="MNLKSIIWVMAGVGALMASPAWTADLKIAVVDFGRLLEDSPQYK
                     VAQESLRTEFTPRQRELQNQQNSLKAKEDKLQKDGATMTPDQRATVEKELRDSYRELQ
                     RKQSEVQDDLTARRNEEMSRLQKTVIEQVRTYAKSQNFDLVLADGVIYTTPTIDITSQ
                     VLQTLQSHTGSTKPAATAPAPAPAKPQSH"
     gene            complement(6743..9115)
                     /gene="bamA"
                     /locus_tag="VFB37_16770"
     CDS             complement(6743..9115)
                     /gene="bamA"
                     /locus_tag="VFB37_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZO24150.1"
                     /translation="MSPPSAPPSESDFTVGDIRVEGLQRVSEGTVYNYLPVNIGDHLN
                     PTRIREAIRALYATGFFRDVQLRREGNTLIVVVLERPSIESFEISGNKDIKTEDLQKS
                     LRNVGLATGKTFDRSVLEDVTGYLTDQYFSRGKYGVRVDTHVEEETGNRVKIKIEIKE
                     GKRAKIREINLVGNRTYKPKEILDTFELHTPNWKSWYKQDDRYSRESLQGDLEKLRNF
                     YMDRGYANFQIDSTQVAIAPEKDDIFITVNLEEGDVFKISDVKLAGTFVVPKAELERL
                     VLVKPGQIFSHKLITSTQELIQNRLGADGYAFAKVDPVPTTNNTTHEVSLTFFIDPGN
                     RVYVRSITFSGSTRINDEVLRRELRQLEGGWLSNTSLERSKQRIQRLPYVKTVEYETT
                     PVTGAPDLVDVNYKLEEGPSAQLGGGIGYSETYKFQLNGSYTDANFLGTGQRISLDLN
                     GGAFSKVYNLSHTDPYTSVDGVSRTESLTYRDVTQFVSSSSNFSSKTLSAGLTWDYPL
                     TEYQYFRFGGTLDSAELLTNSLGSALQATQWVQQNGHSYTRLAHDDTTNNEFVFYGTN
                     FKSAELLTGWGIDTRNRTLFADRGMRQTISVSSTVPGSGVNYWVGDYSFLQYVPIRGP
                     WLLSFSFAVDYGQPFRGTTALPPYRNFFGGGPDTIRGYRESRLGPKDQFGNPYGGNLR
                     VVNRNELVLPIPAKWRQTARLSVFFDQGNVFSTTKALKFFGPDQVDPADYNFSLKDIK
                     QSVGVSVEWLAPLGLFRFSLGLPLNPTHYNGLDKWGDETEVFQFSIGQAF"
     gene            complement(9481..10863)
                     /gene="rseP"
                     /locus_tag="VFB37_16775"
     CDS             complement(9481..10863)
                     /gene="rseP"
                     /locus_tag="VFB37_16775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZO24151.1"
                     /translation="MSFAWHALWFVVAVSLLVTVHEFGHYWVARRLGFKVLRFSVGFG
                     KPLLRRTAGPDHTEYVVAAIPLGGYVKLLDEREGPVPPQDLDRSFTRRPPWQRIVVLL
                     AGPAFNIIFAILVLWGMFWANGVNDVRPVVGDVMQDSIAAHGGLRSGDEIVAVDGSHV
                     GGQLDVVFDLLDAVSSRGRVTLSVHGREGGDRVIALDMPDREERVRLTEPAALFKGLG
                     FQFWTPSVPAQVRQVVAGGPAERAGLKAGDRILSIDGRPVDFNHFVGNIEDNLGHTIV
                     IGYRRGGVENNARVVVAAERVDGKTIGRINLVVEPVPGLQIKYPPGMLTHVALSPPAA
                     LSRAAHEAWSMSALQGRLFWRMVLGQVSMKNLNGPLSIAEYAGDSAEAGIASFMSFLV
                     LISLSLGFLNLLPIPILDGGQIVFQLVEWLKGSPLSDRAQAFGQQIGIALLILLMGVA
                     LFNDIARQFS"
     gene            complement(10898..12067)
                     /gene="ispC"
                     /locus_tag="VFB37_16780"
     CDS             complement(10898..12067)
                     /gene="ispC"
                     /locus_tag="VFB37_16780"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZO24152.1"
                     /translation="MKGVVVLGSTGSVGENTLDVLGRHPERFRLVGIGANRNAARLAE
                     QVLKWRPAYAALADENAARELQQLLGQQAPGTRVLAGEQGLIELAGLREVDIVMAAIV
                     GAAGLRSTLAAARAGKRVLLANKESLVMAGPLMMAAVRSSGATLLPIDSEHNAIFQCL
                     PPQARTGEFPPGVRRILLTASGGPFRDVPREALESVTPEAACAHPNWVMGRKISVDSA
                     TLMNKGLELIEACFLFGVTPQDVEIVVHPQSIVHSLVEYLDGTVLAQLGSPDMRTPIA
                     HALAWPERIASGVEFLDLVRTARLDFRAPDYEKFHCLALAQAAARAGGLQPVILNAVN
                     EVAVQAFLERRLNFIEIAAVIEAVITEADGGEIGSLDDVLAADAQARQLAGERLQ"
     gene            complement(12064..12876)
                     /locus_tag="VFB37_16785"
     CDS             complement(12064..12876)
                     /locus_tag="VFB37_16785"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZO24153.1"
                     /translation="MSEGLRKRITTAIILAAVLFAVALWLPARWIAGVLTLLMLAGAW
                     EWSALLRFRSNALRAAYLAFLAAMMPVVWHVTATPEGRDIVLVSAVVWWIVALGWIAF
                     APQFVAPWSAALAGALALLPAWIALLRLRLDLPRGAEWMLFTLILVFAADIGAFFVGR
                     RFGRLRLAPTVSPGKTWEGVLGGALAGAVIAVLGSGWFRVPLQTFLPLCLAAVGFSIV
                     GDLTESLLKRFAGVKDSGTLFPGHGGVMDRIDSVTAAAPVLLLGLRVLGVAT"
     gene            complement(12873..>13102)
                     /locus_tag="VFB37_16790"
     CDS             complement(12873..>13102)
                     /locus_tag="VFB37_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HZO24154.1"
                     /translation="DLMIRTGGEQRISNFLLWQGAYAELFFTPALWPDFDLAELESAF
                     DFFQGRERRFGGTSEQLPAAQGVAAMKARET"
BASE COUNT         2395 a         4367 c         4214 g         2126 t
ORIGIN      
        1 accggtccgg ctgtaaccgg cgcaccagat ccttcatgcc gcgcgattcg agttggaaaa
       61 cggcggtggt gctgcaggcc ttcagcagtg caaacgtgcg gggatcgtcc atcggcagcg
      121 caccgatctc caggggcggc tcgccgcgct gcgcacggat tccgttgatg gtcttcaccg
      181 cggagtggat gactgtcagg gtccgcagac cgaggaaatc gaacttcacc agtcccgcgg
      241 cttccacatc gtccttgtcg aactgcgtga cgacatggcc tgccgcctcg tcacagtaga
      301 ggggagcgaa gtccgtgagc acggaaggcg cgatcacaac accgcccgcg tgcatgccgg
      361 cgttacgggt caggccctcg agtgagcggg cgagatcgat gaggttgcgg acctcgtcct
      421 cgctatcgta gaggcgcttg agctcgggct ccttcgcgag cgcatcctcg agcgtgatcc
      481 cgagctcgaa cggaatcagc ttcgcgatcc ggtcgacgta tccgtaactc atgccaagca
      541 cgcggccgac gtcgcggacc acggccttgg ccgccatcgt tccgtacgtg atgatctgcg
      601 aaacacgctc ccggccgtac ttctgcgcca catagtcgat gacgcgatcg cggccatcca
      661 tgcagaaatc aacatcgaag tcgggcatcg agacccgctc cggattgagg aagcgctcga
      721 acagcaaatc gtggtggatg ggatcgagat cggtgatgcc caggctgtag gcgaccagcg
      781 agccggcgcc cgagccgcgt cccgggccca ccggaatgtg gttctcgcgc gcccagcgga
      841 tgaaatccgc cacgatcagg aaatatcccg caaagcccat ctgacagatc acatcgagct
      901 cgagccggag ccgttcgcga tagagctcga cgggtgcggc ggacgggccg gcaaggcgcg
      961 cttcgagccc gcgggaagcc tcctccctca cgaactgttc ggtgctgaga ccggccggaa
     1021 ccggatattg cggcaggcgc gcctctccga gcttcagcac caggctgcaa cgctgcgcga
     1081 tggcaaccgt gttggccaag gcctccggca cgtccgagaa cagggacgcc atctcctgcg
     1141 gcgtgcgcag atactgctgt gcggtatagc ggcgctggcg gccgggatcg gccagcaggg
     1201 ctccatcgtg aatgcagacc cgcgcttcat gcgattcgaa gtcctcggca cgcaggaacc
     1261 gcacatcgtt cgtggccacc agcggaaccg agcagcggct cgccagggat acggcggccg
     1321 cgatgtagcc ctcctcttcc ggatgcccca gccgctgcag ctcgatatag aagcggtcgc
     1381 cgaaaatagc gagccagcgc tcgagcgcct gttccgcttc ccgctcgcgc gcattcacca
     1441 gcgcgcgacc cacatcgcct tgcgtcgcgc atgagagggc gagcagccct tggacgctct
     1501 cttgcgtgag ccagtgagga tccacccagg gcacgcccct ttgctgcccc tcgagatacg
     1561 ccctgctcag caaccgcgtc acattgcggt agccggtttg cgactgacac agcagtgtga
     1621 ggcgaaagcc ctgctgccgc tcgccgggct cgcgcacccg caggtccacg ccgatcagtg
     1681 gcttcacccc cttggcaagc gccgcacggt agaacttcac catggcgaac aggttgttct
     1741 gatcggtgag cgcaacggca ggcatgccgg cggcggctac ggcatcgatc agctcgggca
     1801 cacgcacgac gctgtcgatg agcgagtact cggtgtgcaa gcgcagatga acgaaacccg
     1861 tcagcatcgc agtcagtcca gatcgggact caacaccagc agatgctcgc gaatgggctg
     1921 gaacgagcgc cgatgcagtg gcgagggctc ccggcaagcc agcgcgcgca gatgtcccgg
     1981 ggtgcagtag cccttgtgcg cggcgagatc gaagcccgga tagcaggcgt cgagctgttc
     2041 catcatggca tcgcgacagg tcttcgccag gatggaggct gcactgatcg ccgccacaca
     2101 ggcatcgccc tccacgatcg cctcggcggt gcagcccagg tgcagatcat cgagtcgcgg
     2161 cagcagattg ccatccacct gcacgtgagt gggacacacg ggaaggcgca ggagggcacg
     2221 gcgcatggcg agaaatgtcg cacccaggat gttcaggcag tcgatctcgt cgcggtcgga
     2281 ccaggcgacg gaccaggcga gggcccgggc gcgaatgatc ggcgcgaggc gcgcgcgctc
     2341 ttcaggggtg agcgccttgg aatcggcgag gccccggatg cggcggcgcg ggtccagaat
     2401 cacagcggcg gcgaccacgg gacctgccag tggaccgcgc cctgcctcat ccactccggc
     2461 acagcgcatc ggcccgcacc tgctgcgcgc gcgaagcagc ttcatcgcgt agcgcttgac
     2521 gcgctgggcc ctcatgctct gcctctcaac acgtgcacta tcgtcgctcg cgcgcgcact
     2581 ccagaattgc tgccgcggcg cgctgcgcgc cacccagacg aagagcctcg tgcacggcgc
     2641 gaaattcgcg cctgagctcc tcgacatgcg ccggatcctc gagctcccgc agcagagccg
     2701 cgccgagcgc ttcggcgctc acctgctcct ggaagaattc gggcacccgg gcgcgtccaa
     2761 tcagaagatt cggttgcgag aagtatggca ccttgacgag cccgagtgtg cgcaacagaa
     2821 gtgccgtcag cgcgctcacc cggtacgcca cgaccatggg acgacccgag agcagcgtct
     2881 cgagggtcgc cgtgcccgag gcgacgatcg cggcatccgc ggccgccagt gcggtgtggg
     2941 cgtggccatc gagcagcagg atctcaggcc gcctcgcagt gcgcgccagc tgccgctcga
     3001 acactgcgcg cacgcgagcc gaagccatgg gcgcaacgaa ctggaactcc ggccggcgag
     3061 cgcgcagcca gctcgccgcg gcggcgaaat cactcccgag ccgctccact tcggcgagtc
     3121 gactgcccgg caggagcgcg atcagctgcc gttgcgcatc cagagccagg gcggctcgcg
     3181 ccgcggcacg atcgggcgag agcgggatct gatccgcgag tggatggccg acgaatagcg
     3241 cgcgcacccc gtggcgcacg tagaactccg tttcgaaagg cagcagacac agcaccagat
     3301 cacacgccgc accgatactt cgcacgcgcc cttgccgcca tgcccagacc tgcgggctca
     3361 cgtattgaac ggtacgcatc ccctgacgct tgagacgctt cgccaaaccg aggttgaatg
     3421 ccggggcgtc gatgccgacg aaggcatcgg ggcgcgccgc gccgaagcgc tcgatcagca
     3481 tcgagcgcag ccgcagcagc ctcggcaggt gccgcagcgg ctcggtgaag cccatcacgg
     3541 cgagctcttc ggaggacgcc caggcctcga agcctgcggc ggccattttg ggtcccccga
     3601 cgccgaagca gctcacctgc ggcaccagcg ctttcagtgc cgagatgagt gcagccccga
     3661 gctgatcccc ggaggattcc ccggcaacga gtccgatgga aatgggcgca gcggtcgtgc
     3721 cttgaggccg gccagccgcc gcacccgact gtgggccgcc cgccggaccc gacgccaggg
     3781 cggccgccag acccggccgc ggggcgacgg gcgcccccag ctcgctcatc gaacgatgct
     3841 gcgcgtggag gtgccgatga agtcgacgaa ggcgcggatc tcggaacggc tctccgcgat
     3901 cgggcgcaac ttctcgagtg cttcggtgag acgcaggtcc gagcggtaca ggatccgata
     3961 ggcctcccgt acgttgcgaa tctgctcctc atcgaagccg cgtcgcttga ggccctcggc
     4021 attgactgca tggggctcgg ccggattgcc ggcaaccatc acatacggcg ggacgtcgcg
     4081 ggtcacgatc gccccgccgg cgaggaaggc gtgcgcgcca attttggcga actggtgtac
     4141 ggccgtgagg ccgccgagaa tcacccagtc gccgatctcg acatggccgc ccagagcggc
     4201 gcagttggcg aacaccacgt tgctgcccac caagcagtcg tgggcgacat gcgagtaggc
     4261 catgaacaga ttgtcattgc cgatgcgtgt cacgcccctg tcgtgcgtcg tgccacgatt
     4321 gaccgtgcag ctctcgcgaa acacattcct gtcgccgatc tccagggagg tccgctcgcc
     4381 cttgtacttc ttgtcctggg gagcatcgcc gatggaggcg aactggaaga tccggttatc
     4441 cgcaccgata cgggtcggcc cgttgaggac cacgtggggg ccgatgacgg tacgcggccc
     4501 gatctgcacg tctggaccga tgacactgaa gggacctacg gtcacgtcct ctgccagctg
     4561 cgcttgtggg gagatgagtg cgtgcgcgtc gatcacttgc cggtctccgg cgccaccatc
     4621 atgtccgcgt gggccacctc aacatcgccg accgtggcga ccgtcgagaa gcgccagatg
     4681 cctttcaggg cgcgctcgag ctgggcgaag agaatcagct ggtctccggg ctcgaccggc
     4741 ttgcggaagc gcgccttgtc gatgccgacg aaatagaact tggtctggtg gttcggcacc
     4801 acgccggcgg agacgaaggc gagaatgccc gcagcctgcg cgagcgcctc cacgatgatc
     4861 actccgggca tgagcggccg ccccggaaag tgacccggga aatacggctc gttgtaggtc
     4921 acgttcttga gggcgcggat gcttttgccc ggatggcact cgaggacccg gtccacgagg
     4981 agaaaggggt accggtgcgg cagcagccgc acgacttcgt tgatatccag tcgtatcgga
     5041 tcagtcatca ccctcgtcct gcgcagcgcc ggcggcgcgc tcgagctgct tcagccgctt
     5101 ctcgtacgtc tcgagcttgc ggtaccgagc tacctgccgg cgccacactt tggattcttc
     5161 tatgggaatg ccgctggagt ataccccggg ggatgtcagc gaccgactga ccatggcgaa
     5221 acccgtgatc gtcacaccgt cgcagattgt caggtgaccg gcgattccga ccgccccccc
     5281 gatgatgcac cgccggccga tgatcgtgct gccggaaatt cccgtgcagc cggcgatcac
     5341 cgtgtgcgcc ccaactcgca cgttgtggcc gatctgaatc aggttgtcga gcttggcgcc
     5401 ctcctcgatg accgtatcct cgatcgcgcc ccggtcgatc gtcgtgttgg cgccgatctc
     5461 cacatccggc ccgatgctca cactgcccac ttgcggcacc ttgagccatc ggccgcgatc
     5521 gggggcaaat ccgaacccgt cggctccgat gaccgctccg ggctgaatta gcgtgcgcgc
     5581 cccgacacgc acgccccggc acagggtcac ctttgcaacc agccgcacgt attcttccag
     5641 aacgacatcg ttctcgagga ccgaatgcgc accgacgaaa acatgcgagc cgatgactgc
     5701 gcggtccccg atgaccgcgt gcggtccaac gtgcgcgctc gagtcaaccc gggcatcggg
     5761 cgcgacagtg gctgaggggt ggatgcccgg ctttgcgggt gcgtcggggt agagcagcgc
     5821 ggcaatccgg gcgaaggtcg cccgcgggtt gtcgcagatc agggcggcgg tcggacagaa
     5881 ctgcgcgacg gcggcaccca gcaccacgac agctgcgcgc gtggccgcga gctcccgtct
     5941 gttgcgatct tcagcaagga aggcgacgca cagatcgtcc gcgttgacga gggtcccgac
     6001 acgctcgacg cgcatgtcgg gattgccccg aagctcacag ccaaaacgga ccgccagctc
     6061 cccgagtgta acggccatac ctgggcgacc tgccgttgtt ggccgcgccg gacggctcga
     6121 gcgtcagtgc gactgaggtt tggctggcgc aggagccggc gcggtcgcgg cgggcttggt
     6181 tgagcccgtg tgcgactgca gcgtctgcag gacctgcgaa gtgatgtcga tcgtcggcgt
     6241 ggtgtagatc accccgtcgg cgagcaccag gtcgaaattc tgcgacttcg cataggtccg
     6301 cacctgctcg atcaccgtct tctgcaaccg ggacatctct tcgttgcggc gtgcggtcag
     6361 gtcatcctgg acctcactct gtttgcgctg cagctcgcgg tagctgtcgc gcagctcctt
     6421 ctccaccgtc gcgcgctggt ccggtgtcat cgtcgcgcca tccttctgca gcttgtcttc
     6481 cttggccttg aggctgttct gctggttctg cagctcgcgc tggcgcggtg tgaactcggt
     6541 gcggagcgac tcctgagcca ccttgtactg cggcgagtcc tccagcagac gcccgaaatc
     6601 cacgacggcg atcttcagat ccgccgtcca cgccggcgag gccatcagcg caccgacccc
     6661 cgccatgacc catatgatcg atttgagatt catcgcaacc ttcacggata acccacctgg
     6721 agtagatgcc gcctattgtg gcctaaaacg cctgtccgat ggagaactgg aagacttcgg
     6781 tctcatcgcc ccacttgtcc agcccgttgt aatgggtcgg attgagcggc agtcccaagc
     6841 tgaatcggaa cagccccagt ggcgccagcc actccaccga gacccctacc gactgcttga
     6901 tgtccttgag actgaaattg tagtcggcgg gatcgacctg atccggaccg aagaatttca
     6961 gggccttcgt ggtcgagaac acgttgccct ggtcgaagaa cacgctcagg cgggcggtct
     7021 ggcgccactt ggcggggatg ggcaggacca gctcgttgcg attgacgacc cgcaggttgc
     7081 cgccataggg gttaccgaac tgatccttgg gccccaggcg gctctcacgg tagccgcgga
     7141 tcgtgtccgg gccgccgccg aagaaattgc gatagggcgg cagcgccgtc gtgccccgga
     7201 aaggctggcc gtaatccacc gcgaagctga acgaaagaag ccaggggccc cggatcggca
     7261 cgtactgcag gaagctgtaa tcgcccaccc agtagttgac gccgcttccg ggtacggtcg
     7321 aggacacgga gatcgtctgg cgcatgccgc gatcggcgaa cagcgtgcgg ttgcgggtgt
     7381 cgatccccca gccggtgagc agctccgccg acttgaaatt ggtgccatag aagacgaatt
     7441 cgttgttcgt cgtgtcatcg tgggcgagac gcgtatacga atggccgttc tgctgcaccc
     7501 actgagtggc ctgcaacgcg ctgcccagcg aattggtcag cagctcggcg ctgtcgagcg
     7561 tgcccccgaa gcggaagtac tggtattccg tcagcgggta atcccaagtc agcccggcac
     7621 tcagagtctt cgaagagaag ttcgaggatg acgagacgaa ttgcgtgaca tcgcggtagg
     7681 tcagcgactc ggtccgggag acgccgtcaa cgctcgtgta cgggtcggta tgcgagaggt
     7741 tgtacacctt gctgaacgcg ccgccgttca gatccaggga aatgcgctgc cccgtgccga
     7801 ggaagttcgc atccgtgtag ctgccgttga gctggaactt ataagtctcc gagtagccga
     7861 tgccgccccc cagctgagcg ctcgggccct cctcgagctt gtagttcaca tccaccagat
     7921 ccggcgcgcc ggtgaccggc gtggtctcgt actcgaccgt cttcacatag ggcaggcgct
     7981 ggatgcgctg cttcgagcgc tccagggaag tgttggagag ccagccgccc tcgagctggc
     8041 gcagctcacg acgcaggacc tcgtcattga ttcgagtcga gcctgagaag gtgatgctgc
     8101 gcacgtacac gcgattgccc ggatcgatga agaaggtcag ggacacctcg tgtgtcgtgt
     8161 tgttcgtggt cgggaccgga tcgactttgg cgaaggcgta tccgtcggct cccaggcggt
     8221 tctgaatgag ctcctgggtg gaggtgatga gcttgtggct gaagatctgg ccgggcttca
     8281 ccagcaccag ccgctcgagc tcggccttcg gcaccacgaa ggtcccggca agcttcacgt
     8341 cggagatctt gaaaacatcc ccctcctcca ggttcacggt gatgaagatg tcgtccttct
     8401 cgggagcgat cgcgacctgg gtggagtcga tctggaaatt cgcatagccg cggtccatgt
     8461 agaagttgcg cagcttctcc agatcgccct gcagcgattc gcgcgagtag cggtcgtcct
     8521 gcttgtacca ggacttccag ttcggggtat gcagctcgaa ggtgtccagg atctccttgg
     8581 gcttgtaggt gcggttaccg accaggttga tttcgcggat cttggcgcgc ttgccctcct
     8641 tgatctcgat cttgatcttc acccggttgc cggtttcctc ctcgacgtga gtatcgaccc
     8701 gcacgccgta cttgccgcgg ctgaagtact ggtccgtcag gtagccggtc acatcctcga
     8761 ggaccgagcg gtcgaaggtc ttgccggtag cgagaccgac gttgcgcagc gacttctgca
     8821 gatcctcggt cttgatgtcc ttgttgccgc tgatctcgaa gctctcgatc gaaggccgct
     8881 cgagcactac gacgatcagg gtattgcctt cgcgccgcag ctggacgtcg cggaagaagc
     8941 cggtcgcata cagggcgcgg atggcctccc ggatacgggt cggattcaga tgatcgccga
     9001 tgttgacggg cagatagttg tagacggtgc cttcggagac tcgctgcagt ccctccacgc
     9061 ggatgtcgcc gacggtgaag tccgattcgc tcggcggagc cgagggagga gacaccggtg
     9121 caggggcggg cgcggctgcc ggggccggct gcgtggctgt tggggctccc tgcgaggagg
     9181 tcggggctgg cgctggctgg gcggccgccg gtgatggctg cggcggcgca ggcgcccgca
     9241 ccggcccctc cgtcgcggga gtgctcggtg cgggggttgc aggcgtttgc gcgtgcgcga
     9301 acgtgcacgg cgccagcagg gcaaccgtgg ccaggacggc gcgagccttc atgcttcaat
     9361 caccggtaac tttcttcttc gcgccgtgtg cgcctggatt ccaacatgta cccccgcgcc
     9421 gcccgccgac tcgcagcgca ggcgtggccg ccggccctcg tgtttcctcg accgagatcg
     9481 tcaactgaac tgacgcgcga tgtcgttgaa caacgcgaca cccatcaaca ggatgagcag
     9541 cgcgattccg atctgctgcc cgaaggcctg tgcccgatcc gacagcgggc tgcccttgag
     9601 ccactcgacg agctggaaga cgatctgacc gccatcgaga atgggaatgg gcaacaggtt
     9661 gagaaatccc agcgacagcg agatcagcac caggaagctc atgaacgagg caatgcccgc
     9721 ctcggccgaa tcgcccgcat actcggcgat ggacagcgga ccattcaggt tcttcatcga
     9781 gacctggccc agcaccatcc gccagaagag cctcccctgc agcgcgctca tgctccaggc
     9841 ttcgtgcgcg gcgcggctca aagccgcagg cggagaaagc gccacgtgag tgagcatgcc
     9901 gggcgggtac ttgatctgca ggcccggaac gggctcgacc accaggttga tgcggccaat
     9961 ggtcttgccg tccacgcgct cggcggcgac cacgacccgg gcattgttct cgacgccacc
    10021 gcggcgatag ccgatcacga tcgtgtggcc gaggttgtcc tcgatgttgc ccacgaagtg
    10081 attgaagtcc acgggccggc catcgatgct cagaatacgg tcgcccgctt tcagtccggc
    10141 acgctcggcc gggccaccgg cgaccacctg gcgtacctgc gccggaaccg agggcgtcca
    10201 gaactggaac cccagcccct tgaacagcgc cgcgggctcg gtgagccgca cgcgctcctc
    10261 acgatccggc atgtcgagcg cgatgacgcg gtcgccgccc tcgcgtccat gcaccgagag
    10321 cgtgacacgc ccgcgcgagc tcacggcatc cagcagatca aacaccacat cgagctggcc
    10381 gccgacgtgg gagccatcga cggcgacgat ctcatcaccg gagcgcagac cgccgtgcgc
    10441 ggcaatcgaa tcctgcatga catcgccgac gacgggtcgt acgtcattga cgccattcgc
    10501 ccagaacatg ccccacagca cgagaatggc gaaaatgatg ttgaaggcgg ggcctgcgag
    10561 gagcacgacg atccgctgcc aggggggcct gcgcgtgaag gagcgatcca ggtcctgggg
    10621 cggcaccggg ccttcgcgct catcgagaag cttgacgtag cccccgagcg gaatggccgc
    10681 cacgacatac tcggtgtgat cggggcctgc ggtccggcgc agcagcggct tgccgaagcc
    10741 gaccgagaag cgcagcacct tgaaaccgag ccgccgggcc acccagtagt gcccgaactc
    10801 gtgcaccgtg accagcaagc tcacggccac tacgaaccac agcgcgtgcc acgcgaaact
    10861 catttgtgag ccgccctcac caagctctcg ttacatttca ttgcaaccgc tcccctgcga
    10921 gctgtcgcgc ctgcgcatca gcggccagga cgtcatcgag actcccgatc tcaccgccgt
    10981 ccgcctcggt gatcaccgct tcaatgaccg ccgcgatctc gataaagttc aatcgccgct
    11041 cgaggaaagc ctgcaccgcc acttcattga cagcattgag aatcaccggc tgcagccccc
    11101 ccgcccgagc cgccgcctgc gccagcgcca gacaatggaa tttctcgtaa tcgggcgccc
    11161 gaaaatccag gcgcgcggtg cggaccagat cgaggaattc tacaccgctg gcgatgcgtt
    11221 ctggccacgc cagcgcgtga gcgatcggcg tgcgcatgtc cggcgagccg agctgcgcga
    11281 gcacggtgcc atcgagatac tcgaccagcg agtgcacgat gctctgcgga tgcaccacga
    11341 tctccacgtc ctggggtgtt acgccgaaga ggaaacatgc ctcgatgagc tcgagcccct
    11401 tgttcatcag cgtggccgaa tcgaccgaga tcttgcgtcc catgacccag ttgggatggg
    11461 cgcaagcggc ttccggggtc accgattcga gcgcctcgcg cggcacgtca cggaagggac
    11521 cgccggaagc ggtgagcaga atgcgccgca cacccggcgg gaactccccg gtccgggcct
    11581 gaggtggcag acactggaag atggcattgt gctcactgtc gatcggcagg agcgtcgccc
    11641 ctgaggatcg cacggcagcc atcatcaggg gacctgccat gaccaacgac tccttgttag
    11701 cgagcagcac acgcttgcct gcgcgcgcgg cggcgagagt cgagcgcaga cccgccgctc
    11761 cgacgatcgc cgccatcacg atgtccactt cgcgcagccc ggcgagctcg atcaatccct
    11821 gctcccccgc cagcacgcgg gtgcccggcg cctgctgtcc aagcagctgc tgcagctcac
    11881 gcgcggcatt ctcatcggcc agcgccgcgt aggcgggccg ccacttcagc acctgctcgg
    11941 ccagacgcgc cgcgttgcga ttcgctccga tgccgacgag cctgaagcgc tcgggatggc
    12001 gcccgagcac atcgagggtg ttctcgccaa ccgagccggt cgaaccgagc accacgacgc
    12061 ccttcatgtc gcgactccca gtacgcgcag acccagcagg agcaccgggg cggcggccgt
    12121 cacgctgtcg atgcgatcca tcacaccgcc atggcccgga aagagcgtgc cgctgtcctt
    12181 cactcccgca aaccgcttca agagactctc cgtcagatca ccgacgatgg agaagccgac
    12241 cgccgccaga cacaggggca ggaacgtctg cagcggcacg cggaaccatc cgctgcccag
    12301 cacggcgatc acggcgccag cgagtgcccc gcccagtacg ccttcccagg ttttgccagg
    12361 cgacaccgtg ggagccaggc gcaaccgacc aaaacgacgg ccgacgaaaa acgctccgat
    12421 gtcggcggca aaaaccagga tcaatgtaaa cagcatccac tccgcgccgc gcggcaggtc
    12481 cagccgcagc cgcagcagtg cgatccacgc cggcaacaga gccagcgcac ccgccaacgc
    12541 tgccgaccac ggcgccacga actgcggcgc aaacgcgatc caccccaatg ccacgatcca
    12601 ccacacgacc gcggagacga gcacgatatc ccgtccttcg ggcgtagcgg taacgtgcca
    12661 cacaacgggc atcatcgcag ccagaaacgc taggtaggcg gcacgtaacg cattggagcg
    12721 aaaacgcagc agcgccgacc actcccaagc accggcgagc atcagcagcg tcaggacccc
    12781 agcgatccag cgcgccggaa gccacagcgc gacggcgaag agaaccgcgg cgaggatgat
    12841 cgccgtcgta atgcgcttgc gcagaccctc gctcacgtct cgcgggcttt cattgccgcg
    12901 acgccttgcg cggcgggcaa ctgctcggag gtgcctccga agcgtcgctc tcggccctgg
    12961 aagaaatcga aggcggactc gagctcggcg agatcgaagt ccggccacaa agcgggagtg
    13021 aaaaacagct ccgcataggc tccctgccag agcaggaaat tgctgatgcg ctgctcaccc
    13081 ccggtgcgga tcatgagatc gg
//
LOCUS       DASRHN010000417         4777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_321136, whole genome shotgun sequence.
ACCESSION   DASRHN010000417 DASRHN010000000
VERSION     DASRHN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321136"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3996)
                     /locus_tag="VFB37_16795"
     CDS             complement(<1..3996)
                     /locus_tag="VFB37_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZO24155.1"
                     /translation="MPADRPRAGASAPRAVPVNRSPLSFAQQRLWFLSQIEGASEAYH
                     IFSAWRLLGRLNSGALRKALDRIIVRHEALRTTFAVVDAAPVQQVAPAPGCRFALVEE
                     DWRTRTDSAEALERLMRQESVRPFDLRTGPMIRGYLVRLTDMEHALLVTMHHIVSDGW
                     STGVLINEFCALYGAFCEGRPDPLPPLALQYPGYSLWQRRFLEGPRMQQHVEYWRKAL
                     AGAPTILELPADFPRPPRQQFAGSFAELRIDEALTVRLKEMSRKHGVTLYMTLLAAWA
                     LLSGRLSGQSDVIIGTPVANRERTELQPMIGLFVNTLALRLNVEGSQTVAQLLKQVRV
                     DTFAAFQHQDIPFEQVVDVVQPKRTLAHSPLFQVMFQWQALPGGALTLPELRVEPMRS
                     AEAMPASKFDMTLSLGEINGGIAGGIEYATALFAPETVAMYGRYLRALLEAMAADDTR
                     SLGHLPLLSAMERQRLVAQWNQSTAAAPTQRYVHELFEDQASATPDALAVVSAGRNLS
                     YRELNERASRLASYLRTHGVGPDDRVAICVERSPEMLISILGVLKAGGSYVPVDPGYP
                     ADRMAYMLEECAPRLILTQECVKQKLPPALPAVAIDLEWSEIESCLPFEGDRVTTGLM
                     PENLAYVIFTSGSTGRPKGTSVEHRGLSNLLQWYTGEMAGIDLNRVLIVSSFSFDLTQ
                     KNLLVPLLTGGQVHLAAEPFDPAAIARQIEAEGITAMNLTPSALYAILESCDARRLSS
                     LRKVVLGGEAITAVRLAEFARACPQVQIINNYGPTESTDVASVCTLPGADELARGPQI
                     PIGRALPNMRLYILDAHGEPVPTGVSGELYLGGIGVARGYLHRPELTAQRFVADAFSD
                     IPGARAYRTGDLARYRRDGQIEFQGRNDFQVKLRGYRIELGEIEAGLLAFEGIRQAVV
                     VAREDSTGDKRLVAYYTATPGGEAVTAEQLRTHLSSLLPAYMVPAAYVRLEQLPLTPS
                     GKLDRRSLPSPDASAYAAPEYEAPQGPMETTMAAIWGELLEVDRIGRHDDFFERGGHS
                     LLMLKLVMRLQQAFGLDVKITDVFEHPVLADLARALQNARGQRAILPPITRADRRQRL
                     PLTFAQQRLWFLAQMEGGSDAYNLSFGMRLEGQLNYAALRRALDRIIARHESLRTTFT
                     FEDGTPAQRIAPIEESRFHLVEHDLSRDPGWKMRVLQLEQEEARQSFDFVRGPLIRGL
                     LIRFSDRTHVLRISLHHVICDGWSLRVVRREIAALYDAFSNDQPDPLPELPIQHADFA
                     VWQRQWSGGDVLGRQGEYWKARLAGAPALLELPTDHPRPPRQDYAGSVAKVVLDEKLS
                     GAIRVLARRHDA"
     gene            complement(4032..>4777)
                     /locus_tag="VFB37_16800"
     CDS             complement(4032..>4777)
                     /locus_tag="VFB37_16800"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZO24156.1"
                     /translation="QAERCIQRASGYLGRLVHKAIAKPVKVSPMIAHLQAFAGQGYYE
                     QPSCLTGGYGVFHLNVSKARPNSETLQTLTAHETIPGHHLQAVYTYERSPSLPAFRSV
                     LPFDGYCEGWAVYAERLLAELGFYEGDPRGELGRLQSDMQRSVRLLLDTGIHHHGWGV
                     AQAAHTVREMVGDPEWLPEEIERVCIQPAQGAAYKCGELALLDLRERARTARGAEFDL
                     RTFHHEVLEIGPCPLPLLEQVIGGGGGAQ"
BASE COUNT          893 a         1611 c         1451 g          822 t
ORIGIN      
        1 agcgtcgtgc cggcgcgcga gcacccggat ggccccagag agtttctcat ccagaaccac
       61 cttagccacg ctgccggcat agtcctgtcg tggcggacgc ggatgatccg tcggcagctc
      121 cagcagcgcc ggtgcaccgg cgagccgtgc cttccagtac tcgccctgcc ggcccagcac
      181 gtcccctccg gaccactgcc gttgccacac cgcgaaatcg gcatgttgta tgggaagttc
      241 gggcagcggg tccggctgat cgttcgagaa cgcgtcgtac agcgcagcaa tctctctgcg
      301 caccacacga agcgaccagc cgtcgcagat cacatgatgg aggctgatcc ggagcacgtg
      361 ggtacgatcg ctgaatcgga tgagcaggcc gcgaatgagc gggccgcgca cgaaatcgaa
      421 gctctggcgc gcttcctcct gctccagctg cagcactcgc atcttccaac ccggatccct
      481 gctcagatcg tgctcgacca gatgaaagcg gctctcctcg atgggtgcga tgcgctgcgc
      541 cggcgtgccg tcctcgaagg tgaatgtcgt gcgcaacgac tcgtggcggg caatgatgcg
      601 gtccagggcg cgccgcaacg cggcataatt cagctgccct tccaggcgca tgccgaatga
      661 aaggttatag gcatcgctcc ccccttccat ttgcgccaga aaccacaggc gctgctgcgc
      721 gaaggtcaac ggcaggcgct gacggcgatc cgctcgcgtg atcggcggaa ggatggccct
      781 ttggccgcgc gcgttctgca gcgctctggc cagatcagcc agtaccggat gctcgaacac
      841 atcggtgatc ttcacatcca ggccgaaagc ctgctgcaag cgcatcacca gcttcagcat
      901 cagcaacgag tggccgccgc gttcgaagaa atcgtcgtgc cgcccgatgc gatcgacttc
      961 caggagctca ccccagatcg ccgccatggt ggtctccatc ggaccctgcg gcgcctcgta
     1021 ttcgggtgcc gcgtaggcgc ttgcatctgg agacggcagt gaccggcggt cgagtttgcc
     1081 gctgggggtc agcggcagct gctcgagtcg cacgtaggcc gcaggcacca tgtaggccgg
     1141 cagcaacgaa gacagatgcg ttcgcagctg ctccgctgtg acagcttcgc cgccgggggt
     1201 agcggtgtaa taggcgacca gtctcttgtc ccccgtgctg tcctcgcggg ctacgaccac
     1261 agcctgccgt ataccttcga acgccaggag gcctgcttcg atttccccca gttcgatccg
     1321 gtagccgcgc agcttcacct gaaaatcgtt gcgaccctgg aattcgatct ggccatctcg
     1381 ccggtagcgc gccagatccc cggtgcgata ggcccgcgcg cccggtatgt cactgaatgc
     1441 atcggcgacg aatcgctgcg ccgtcagctc gggacggtgc aggtagcccc gcgcgactcc
     1501 gattccgccg aggtacagtt ctcccgatac ccccgttggg accggctcgc catgagcatc
     1561 cagaatgtac aggcgcatgt tgggcagcgc gcgcccgatc ggaatctgcg gtccgcgggc
     1621 aagctcatct gcaccgggca gcgtgcatac ggatgccacg tccgtactct ctgtcggccc
     1681 gtagttgttg atgatctgta cctgaggaca tgctcgcgcg aactccgcca ggcgcacagc
     1741 agtgatggcc tccccgccga ggaccacctt tcgaaggctc gaaagccggc gcgcgtcgca
     1801 gctctccagg atcgcgtaga gcgcgctcgg agtgagattc atggcggtaa tcccctccgc
     1861 ctcgatctgc cgcgcgatgg cggcaggatc gaaaggctcc gccgccagat gaacctgacc
     1921 acccgtcagc aacggaacga gcaggttctt ctgtgtcaga tcgaaactga aggagctgac
     1981 gatcagcacc cggttcaggt caatgccggc catttccccg gtgtaccact gcaacagatt
     2041 gctcaggccc cgatgctcga cgctggtgcc cttgggccgt ccagtcgatc ccgaggtgaa
     2101 gatgacgtat gcgaggttct ccggcatcag acccgtcgtg acccgatcgc cttcgaaggg
     2161 aaggcagctc tcgatctcac tccattcgag gtcgatcgcc accgccggca gggcgggtgg
     2221 cagtttctgt ttgacgcatt cctgcgtgag gatcaggcgg ggcgcgcact cctcgagcat
     2281 gtacgccatc cggtccgcag ggtagccagg atccaccgga acgtaggagc ccccggcctt
     2341 gagcacgccc agaatggaaa tcaacatttc cgggctgcgc tcgacgcaga tcgccacacg
     2401 atcatccggc cccacgccgt gggttctcag gtaagacgcc aggcggctcg cccgctcatt
     2461 caactcccgg tagctgagat tcctgcccgc actcacgacc gctaaagcgt ctggcgtcgc
     2521 agacgcctga tcttcaaaca gctcgtgaac gtagcgttgg gtgggagcgg ctgcagtcga
     2581 ctggttccac tgtgccacca accgctgccg ttccatggcg ctcagcaacg gcaggtgacc
     2641 caggctccgc gtatcgtcgg ccgccatcgc ctccagcaac gcacgcagat agcggccgta
     2701 catggcgacc gtttccggcg caaacagagc ggtagcgtat tcgatgcctc ctgctatacc
     2761 cccgttgatt tcgcccagcg acagggtcat gtcgaacttc gaggcgggca tcgcctccgc
     2821 cgaccgcatc ggttccacac gcagctcagg tagtgtcagc gcgccccccg gcaaggcttg
     2881 ccactgaaac atcacctgga ataacgggct gtgagccaga gtgcgcttgg gttggacgac
     2941 gtcgacgacc tgctcgaacg gaatgtcctg gtgctgaaac gcggcgaagg tatcaacccg
     3001 cacctgtttc agcagttgcg ctacggtctg cgagccctcc acattcaggc gcaacgcgag
     3061 cgtgttgacg aagagcccga tcatgggctg caactcggtc cgctcccgat tggccacagg
     3121 cgtcccgatg atcacatccg actgaccgga aagacggccg gacaacaagg cccaagccgc
     3181 gagcaacgtc atatacaacg tgacgccgtg cttgcgactc atctccttga gtcggaccgt
     3241 cagcgcctca tcgatccgca gttccgcaaa actccctgca aactgctgcc gcgggggccg
     3301 cgggaaatcg gcaggtaatt ccaggatcgt cggcgccccc gccagcgcct tgcgccagta
     3361 ctccacatgc tgctgcatcc tcggcccttc gaggaagcgc cgctgccaaa gcgagtaccc
     3421 cggatactgc agcgccaggg gcggcaaggg atccggacga ccttcgcaga atgcgccata
     3481 caacgcgcag aactcgttga tcaggacacc cgtggaccag ccgtccgaga cgatgtgatg
     3541 catcgttacg agcagcgcat gctccatgtc cgtcagccgc accagatagc cgcgaatcat
     3601 cgggccggtc cgcaggtcga agggccggac cgattcctgc cgcatcaggc gctccagcgc
     3661 ctcggcgctg tcggtgcgag tacgccaatc ctcctcgacg agggcaaacc tgcaaccggg
     3721 cgcgggcgcg acttgctgca caggagccgc gtcgaccact gcaaaggtgg tgcgtaacgc
     3781 ctcgtgccgg acaatgatcc ggtcgagcgc cttgcgcagt gcgccgctgt tcaggcgccc
     3841 gagcagacgc caggcgctga agatgtgata agcctcactc gctccctcga tctgagagag
     3901 aaaccacagg cgctgctgag caaaagacaa cggactgcgg ttgacaggca cggcgcgagg
     3961 cgcactcgcg cccgcgcgcg ggcggtcggc aggcatggcc tgcgcttccc gtcgggtcgc
     4021 tccctcattg ctcattgcgc gccgcctccc ccaccgatga cctgctccag caacggcaag
     4081 gggcaggggc cgatctccaa cacttcatga tgaaacgtcc tcagatcgaa ctcagcgccg
     4141 cgggccgttc gggcgcgctc gcgcaagtcc agcagcgcca gctcgccgca cttgtaggcc
     4201 gctccctgcg cgggctgaat gcaaacccgc tcgatttcct ccggtagcca ctcgggatct
     4261 cccaccatct cgcgaacggt gtgcgctgcc tgcgcgacac cccaaccgtg atggtggatg
     4321 cccgtgtcga gcaacaggcg cacggagcgc tgcatgtcgc tttgcagccg cccgagctca
     4381 cctcgcggat cgccctcgta gaagccaagc tccgccagca acctctcggc gtagacagcc
     4441 caaccttcgc aataaccgtc gaacggcaga acggatcgga acgcgggcaa tgaaggggat
     4501 cgttcgtatg tgtagaccgc ttgcaggtga tgtccgggga tcgtctcgtg cgccgtcagg
     4561 gtctgcagcg tctcgctgtt tgggcgggcc ttgctgacat tgagatgaaa gactccgtag
     4621 ccaccagtca gacacgaggg ctgctcgtaa tacccctgcc ccgcgaacgc ctgaaggtga
     4681 gcaatcatcg gactcacctt gaccggcttc gcaatcgcct tgtgcacgag gcggccgaga
     4741 taaccggaag ccctctggat acaacgctcg gcctgct
//
LOCUS       DASRHN010000418         5195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_646360, whole genome shotgun sequence.
ACCESSION   DASRHN010000418 DASRHN010000000
VERSION     DASRHN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646360"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFB37_16805"
     CDS             complement(<1..656)
                     /locus_tag="VFB37_16805"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HZO24157.1"
                     /translation="MTGTYNSSLVLLSVLVAIVVSYTALRLAARVAHAHGSSARLWLA
                     GGSVAMGTGIWSMHFVGMLAFSLPIPLSYDVARTFGSLGIGIASSVFALTLASRPRQS
                     LVRLSSGALVMGAGICAMHYVGMSAVQIRPAITYEMRLVAASAAIAVLASFAALWLFA
                     HLGAGSSWRVRLVRAAAAFGMGLAISGMHYTAMAASRFASGSYCLNQGIGGAGVDSHW
                     L"
     gene            776..1465
                     /locus_tag="VFB37_16810"
     CDS             776..1465
                     /locus_tag="VFB37_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZO24158.1"
                     /translation="MRFRMIAVAACALVSLTGCGYNSLQRQDENVKANWAEVVNQYQR
                     RADLIPNLVRTVKGFAAQEQQVLIGVTEARAKVGSIQATPEILNNPEAFARYQAAQGQ
                     LSQALKSLFAVTEAYPQLKSDANFRDLQAQIEGTENRITVARNRYIDSVRDYNTTVRS
                     FPTNLTARLYDFQVKPNFSVANEQAISAPPEVDFGTPTRGQTVPPAGQGTPAGQGAPP
                     AGPPPPAQPAH"
     gene            1465..2349
                     /locus_tag="VFB37_16815"
     CDS             1465..2349
                     /locus_tag="VFB37_16815"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HZO24159.1"
                     /translation="MRGALIALLLAGCASAWGQDLQPVPRLSARVTDLTGTLTAEQQG
                     GLEEKLAAFEARKGVQIAVLVVPTTRPEDIEQFSIRVVDQWRLGRRNVDDGVLLLIAK
                     NDREVRIEVGKGLEGVLTDATANRIIEETLTPLFRQGDFSGGISAGLDRIMRVVDGEP
                     LPPPDRGWQSRSVPGIAHALPFLVIAVLFGSTLLRALLGRGLGAIVCGAGAGALVYLI
                     GQGLFLAVVAALMAFAWSLVAGFGGGSGWSSSPRRSGWGGGFGGGFGGGFGGLGGGGG
                     GFGGGGGGFNGGGASGRW"
     gene            2374..2853
                     /locus_tag="VFB37_16820"
     CDS             2374..2853
                     /locus_tag="VFB37_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZO24160.1"
                     /translation="MRHLFATRWRTRRLFSAPVLRAIEAAIHEAEGNHAGEIRFAVET
                     SLDLAQLWHDQAPRPRALQVFGQIGVWDTAENNGVLIYLLLADRVVEIIADRGIAGRV
                     PQSEWDAVCREMQSQYAVGRFQEGSVMGVQAVGRLLARHFPHRRGAAAELPDQPVLL"
     gene            2879..4234
                     /locus_tag="VFB37_16825"
     CDS             2879..4234
                     /locus_tag="VFB37_16825"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZO24161.1"
                     /translation="MPYPSLMKSSIRFARAAIAACFCLSVVACSLLGQPWTKRHAPPP
                     PPAPPPAPLEAAPVATQQFELEPNQDIVGEVQLTTTSKDDTLTDVARRFNVGYEEILR
                     ANPKVDPWLPGADREIVVPTQFILPNAPHEGIVINIAAMRLFYFPKHKSGEPQVVVTH
                     PIGIGKVGWATPEGVTHIIGRQKDPTWRVPVSVLKEHRENGEILQPVIGPGPDNPLGR
                     HAFYLQWPSYLIHGTNKPAGVGLRSSHGCIRLYPEDIAQLFDMVPIGTQVRVVNQPFV
                     FGWQNGQLYMQAFDVLEDDPRDWKKAQKKLIGRSVAATLQKQLKSHNEQVDWQVVSTL
                     SHEPRGIPVPISESDATVDQVLAAAPKVQNRVPEGSTWDGTTDLPMDEATFRQVISEM
                     EPPKPGAAPGAPGTKGDPALDRPKASEKTADPAPASPAATAPPEPPSSSTSHARGGPP
                     Q"
     gene            4609..>5195
                     /locus_tag="VFB37_16830"
     CDS             4609..>5195
                     /locus_tag="VFB37_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24162.1"
                     /translation="MSIDSHPAGLPDAGQDLAGHAITRVPTGIAAFVGRTLKGPVNRP
                     VAISSFADFQRVFGGLWQPSTLSYAVDHFFENGGRSAFVVRVANSARPPTITLPAGSA
                     SLRLIGLNPGSREFLRASVDYDAIADNDDEHFNLVVQRVRSAGSEMIEDQEIFRRLSV
                     REESSRHVTDILLESRLARVTGELPLHRPDRSPGGR"
BASE COUNT          898 a         1709 c         1743 g          845 t
ORIGIN      
        1 agccagtgac tgtcgacgcc cgcacccccg attccctgat tcaggcagta cgagcctgag
       61 gcaaaccggg aggcggccat cgccgtgtag tgcatcccgc tgatcgccag tcccatcccg
      121 aatgccgccg cggcccgcac cagccgcacc cgccaggaac tgccggctcc gaggtgagcg
      181 aacagccaca gagcggcgaa tgaggcaagg accgcaattg ctgcggaggc ggccaccagc
      241 cgcatctcat acgtgatcgc cggccggatc tgcacggccg acatgcccac gtagtgcatg
      301 gcgcagatcc ccgcgcccat caccagggca ccgctcgaga gccggaccag gctctggcgg
      361 gggcggctgg ccaaggtcaa ggcaaagacc gagctcgcga ttccgatccc gagggagccg
      421 aaagtccggg cgacgtcgta ggacaggggg attggcagcg agaaggccag catccccacg
      481 aagtgcatgg accagattcc ggtgcccatg gccaccgagc cgcctgcgag ccacagccga
      541 gcggacgagc catgggcgtg cgccacccgc gcagccagcc gcagagccgt gtacgagaca
      601 acgatggcga ccaggaccga gagaaggacg agactggagt tataggtgcc cgtcatgctg
      661 gtccggatat cggcccccgg gggcgccagc ttgaggactt tgggggggta tgtgattgaa
      721 taccgccccg gaggggcagg cgacccgcct gcccgggcga gcccgaggag atccgatgcg
      781 tttccggatg attgctgtgg cggcctgcgc cctcgtgagc ctcacgggct gcggctacaa
      841 ctccctgcag cgccaggatg agaacgtcaa ggcgaactgg gccgaagtcg tgaaccagta
      901 ccagcgccgc gccgacctca ttccgaatct ggtgcgcacc gtgaagggct ttgcggcaca
      961 ggagcagcag gtgctcatcg gcgtcaccga ggcgcgggcc aaggtggggt ccatccaggc
     1021 gacgccggag atcctgaaca atcccgaggc gtttgcgagg taccaggcgg cacaggggca
     1081 gctctcgcag gccctgaaga gcctgtttgc agtcaccgag gcctatccgc agctcaagtc
     1141 agatgcgaac ttccgcgatc tgcaggccca gatcgagggc actgaaaatc gcatcacggt
     1201 cgcgcgcaac cgctacatcg actcggtgcg tgactacaac acgaccgtgc ggtcgtttcc
     1261 aaccaacctc accgcgcgcc tctacgactt tcaggtgaag ccgaatttca gcgtggcgaa
     1321 cgagcaggcg atcagcgcgc cgccggaggt cgatttcggc acgcccaccc gcggccagac
     1381 tgttccgccc gcaggccagg gcacccctgc aggtcagggc gcgccacctg ccggaccgcc
     1441 gccacccgct cagcccgccc actgatgcga ggcgcgctca ttgcgctgct gctggccgga
     1501 tgcgcgagcg cgtggggtca ggatctgcag cccgtgcccc ggctctcggc ccgggtcacc
     1561 gatctcaccg gcacgctgac agccgagcag cagggcggcc tcgaggagaa gctcgctgcc
     1621 ttcgaggcac gcaagggtgt gcagatcgcg gtgctcgtgg tccctaccac gcggcccgag
     1681 gacatcgagc agttctcgat ccgcgtggtc gatcagtggc ggctcggccg ccgcaacgtc
     1741 gatgacggtg tgctgctgct gattgccaaa aatgaccgtg aggtgcgcat cgaggtgggc
     1801 aagggactcg aaggcgtgct gaccgatgcc acggccaatc gcatcatcga ggagactttg
     1861 accccgctgt tcagacaggg cgacttctct ggagggatca gcgcggggct cgaccggatc
     1921 atgcgcgtgg tggatggcga gccgcttccg ccgccggatc ggggctggca gagccggtcg
     1981 gtccccggca tcgctcacgc gctgcctttc ctcgtgatcg cggtgctttt cggctccact
     2041 ttgctgcgcg cattgcttgg gcggggactg ggtgcaatcg tctgtggcgc cggagccggc
     2101 gccctggtct atctcatcgg ccaggggctt ttcctggccg tcgtcgccgc gctgatggcg
     2161 tttgcctggt cactggtcgc cggtttcggg ggcggctccg ggtggtcgag ctctccgcgc
     2221 cgcagcgggt ggggaggcgg tttcgggggc ggtttcggtg gaggattcgg aggattgggc
     2281 gggggcggcg gaggcttcgg tggcgggggc ggcggattca acggcggcgg cgcctcgggc
     2341 cgctggtaag cagcgatgca gcctgcgcgc ctgatgcgac atcttttcgc cacccgctgg
     2401 cgaacgcgtc ggttgttcag tgcgccggtg ctgcgtgcga tcgaggccgc gattcacgag
     2461 gccgaaggga atcatgccgg tgagatccgc ttcgccgtcg aaacctcact ggatctggca
     2521 cagctgtggc acgatcaggc gccgcggccc cgggcgctgc aggtattcgg tcaaatcggc
     2581 gtgtgggaca ccgcggagaa caacggggtc ctcatctatc tgctgcttgc cgatcgggtg
     2641 gtggaaatca tcgcggatcg gggaatcgcc ggccgggttc cgcagtccga atgggatgcg
     2701 gtgtgccgcg aaatgcagtc tcagtacgcc gtgggacgct tccaggaagg gtcggtgatg
     2761 ggtgttcagg cggtgggtcg cctgctggcc cggcacttcc ctcatcgccg aggcgccgcc
     2821 gccgaactgc ccgatcagcc cgtgctgctg tgatccggtg ctcctcacga tatttacgat
     2881 gccctatcct tcgctcatga agtcatcaat ccgtttcgct cgtgccgcga ttgctgcctg
     2941 cttctgcctg tccgtcgtcg cgtgctccct gctgggccaa ccgtggacga agcgtcatgc
     3001 gccgcctccg ccgcccgcgc cgccaccggc gcccctcgaa gccgcgcccg tcgccactca
     3061 gcagttcgag ctcgagccca atcaggacat cgtgggagag gtgcagctca cgacgacgag
     3121 caaggatgac accctgaccg acgtcgcccg gcggttcaac gtggggtatg aggagatcct
     3181 gcgcgccaat cccaaggtgg atccctggct tccaggtgcg gaccgcgaaa tcgtcgtccc
     3241 cacgcaattc atcctgccga acgcgccgca cgaaggcatc gtgatcaaca tcgctgccat
     3301 gcggctgttc tattttccga agcacaagtc cggcgagcct caggttgtcg tgacgcaccc
     3361 catcgggatc ggcaaggtgg gttgggcgac ccccgagggg gtcacgcaca tcatcgggcg
     3421 ccagaaagat cccacctggc gcgtgccagt ctcggtgctc aaggagcatc gcgagaatgg
     3481 cgagatcctg cagccggtga tcggtcccgg gccggacaat ccgctcggcc ggcatgcctt
     3541 ctatctgcag tggccgagct acctgattca tgggaccaac aagcccgctg gcgtcggtct
     3601 gcgctcgagc catggctgca tacgcctgta cccggaggac atcgctcagc ttttcgacat
     3661 ggtcccgatc ggaacgcaag tgcgggtcgt aaaccagcct ttcgtcttcg gctggcagaa
     3721 cggacagctc tacatgcagg ccttcgatgt tctagaggat gacccgcgcg attggaagaa
     3781 agcgcagaag aagctgatcg gccgctccgt cgccgccacg ctgcagaagc agctcaagag
     3841 ccacaacgag caggtggact ggcaggttgt ttccacgctc tcgcatgagc ctcgcggcat
     3901 cccggtgccg atctccgaga gcgatgccac cgtggaccag gtgctcgccg cggcgcccaa
     3961 agtacagaac cgcgtgccgg agggctcgac atgggacggc acgacggacc tgccgatgga
     4021 tgaggcaacg ttccggcagg tgatttccga gatggagccg ccgaagcccg gcgcggcccc
     4081 gggagctccg ggcaccaagg gcgaccccgc gctcgaccgc cccaaggcca gcgagaagac
     4141 cgctgaccct gcacccgcat caccggccgc gacggcgccg cccgagccac cctcgagcag
     4201 cacctcgcac gctcggggtg gcccgcctca gtgaggctgc tggaccgagc cgatgcccgc
     4261 gttttcggag tggctccaga ttatgctcgc ggagatctcg cggaagcgcg aggacgagca
     4321 gcgagcgagc gaagaagaac aagtccgtaa gagcgaaaac ctcacgagcg atgcgccgca
     4381 ggcaccaccg actgctccgg agcaatgagc cgcgcacgct cgggacgctc gtgtccgcgt
     4441 atgctacagt ccgcggcgcc ttagataaca agcgggcaga agacgtctct cgccgccagt
     4501 agagttgagt tgtcggccgg catggcagcg atgctggcat caaggggata acaacatcgg
     4561 ggcgcatact cagccgctca cgcgcactcc tgaggggtgc tctgacgcat gagcattgac
     4621 agccatccgg ccggactccc ggatgcgggc caggatttgg cggggcacgc gatcactcgc
     4681 gtcccgaccg gcatcgccgc ctttgtaggt cgcacactca aaggcccggt gaatcgtccc
     4741 gtggccattt cgagtttcgc cgacttccag cgcgtcttcg gcggcctgtg gcagccttca
     4801 acactttcct acgccgtcga tcactttttc gagaacggtg gccgcagcgc cttcgtggtg
     4861 cgggtggcca actccgcgcg tccgccgacg atcacgctgc cagcgggctc cgcgagtctg
     4921 cgtctgatcg gcctcaatcc cggctcacgc gagttccttc gcgcctcggt cgattacgac
     4981 gcgatcgccg acaacgatga cgagcacttc aacctcgtcg tgcagcgggt ccggtcggcg
     5041 ggctcggaaa tgatcgagga tcaggagatc ttccggcgcc tgtcggtgcg ggaagagtcg
     5101 agccgtcacg tgactgacat cctgctcgag tccaggctgg ctcgcgtgac gggcgagctg
     5161 cccttgcaca gaccggaccg ctcgcccggt ggacg
//
LOCUS       DASRHN010000419        29815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1971538, whole genome shotgun sequence.
ACCESSION   DASRHN010000419 DASRHN010000000
VERSION     DASRHN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 29815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29815
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971538"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VFB37_16835"
     CDS             <1..1009
                     /locus_tag="VFB37_16835"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZO24163.1"
                     /translation="VAVVGATGLVGETMIEVLTERNFPVSELYPLASNRSLGKSVEFR
                     GRSYPVGELAEFDFSKADIGLFSAGAEVSREFAPQAAAAGCVVIDNTSEFRYEDDIPL
                     VVPEVNPQAIAQYTQRGIIANPNCSTIQLVLALKPLHDAVGIERIEVATYQSVSGAGR
                     EALEELAAQSIQLLSGKGPVTARVVAKQIAFNCVPQIDRFEDNGYTREEMKIVWETRK
                     ILDDPQILVNATAVRVPVFYGHSEVVHLQTRTKITAAQARARLSEAPGIEVMDERRAG
                     GYPTAATEAANRDTVYVGRIREDPSSERGLNLWIVADNVRKGAATNSVQIAEILARQH
                     M"
     gene            1120..4113
                     /locus_tag="VFB37_16840"
     CDS             1120..4113
                     /locus_tag="VFB37_16840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZO24164.1"
                     /translation="MVAKSLGRTLALLMALPSAAFAVGLGDIHLLSPLNAPLDAEIEL
                     LDATPEEMQSLKAQLASRDTFARYGLDWPVFLSSVQVHTVRTPDGKEMIKLNSTDAVT
                     EPFITLLVEVNWARGRVVREYTMLLDPPVYTPGQNAGANVPVATPTAGAGVREGSIAR
                     KSDTQTADDPAPATPTAPPSTSASKSAPHAQAVSSGAGDPASAGSTHLIQRGETLSAI
                     AASTAGSPANSPQARSWMLAIYQANPGAFDKNMNLMRSGAVLRIPDSAQVASISASEA
                     SGEIRRQYAAWRSTTPAAPAASASEPGRLRLVTPSQPGAGGAGNGAEVEALQGRVKDL
                     EGQLSDSKRLLELRNAELAKLQAQLAGKNAPAPTQPEAAPAPPAAAPSPPPSAAAPTP
                     PPVEQPAAKAETPAPAPVAQEPPPAAEPTPVPAKPVVAHKPTPASQSQGGSILDSLKD
                     FWWAIVLVLVALAAFIGLRAWRSRRQSEFDDSLGRLAVAGANSMDDGFGTGDTAPVRP
                     LSAASGEGAFLVEESGTHQRPRFPGAAPAAPSAKHVSTDETISSETAINLDQGDPLAE
                     ADFHMAYGLYDQAADLIRIAISREPARRDLKLKLLEVFFVWGNKDQFLQSARELANTR
                     DQAAPGEWEKIVIMGKQLAPEDPLFAGGGAVSGATAGGVDLDLEGGESRVDFDLLGEP
                     VAGQVPGSVDLDIGSALGEPADAGTDSATGATDRSFALQSNAATGTTRQMTQKINRDP
                     EPVLPEFTTETEGPTIEQPGLSTPQNPTIRQKVAMALKQGHSAEQTAELAIDDLGLDL
                     GALDTVDQPGLGASPDAPTLVAGMDDRSRKVIEEAQRRASAEDHDTAATAAFRLDDQD
                     LEAAFGARAANGHGETTAFDTSSTSRLSTLSSRDVDFDLSEGDAGHVAHGTNGAGLDL
                     DVGTATVPDAAFTSTQKLASDDLALPDLEPVTMSEVGTKLDLARAYMDMGDPEGARNI
                     LEEVLHEGSVAQKQEAQRLIESLPG"
     gene            4110..4919
                     /gene="truA"
                     /locus_tag="VFB37_16845"
     CDS             4110..4919
                     /gene="truA"
                     /locus_tag="VFB37_16845"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZO24165.1"
                     /translation="MRLAVGIEYDGAAYAGWQRQPGLHTIQAHLESALSSVAAEPIGL
                     TGAGRTDAGVHARWQVAHFETQSSRPMRGWLLGANTELPRDISILWATPVPAHFHARY
                     SAEARTYRYFILNRGARPALAAKRATWIHKSLDHERMAEAAGLLRGEHDFSAFRSSEC
                     QSRSPVRRLQELIVRREGEWVVIEATANAFLHHMMRNIVGLLTKVGRGDAPPAWAGEV
                     LAGRDRTRAAPTAPPDGLYLWAIRYPNAFALPEPGAGAASGARSAMILGPP"
     gene            4932..5798
                     /gene="accD"
                     /locus_tag="VFB37_16850"
     CDS             4932..5798
                     /gene="accD"
                     /locus_tag="VFB37_16850"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZO24166.1"
                     /translation="MSWFEKIMPSRIKTERRTRSVPEGLWIKCPACDAVLYRAELERN
                     LYVCPKCSHHMRIGARERLESFLDPGETEEIGTRISPEDPLKFRDSKRYKDRLTQAQK
                     ATGERDALIALSGKLQDLPIVACAFEFQFLGGSMGSVVGERFKRAVDRCIEKRQPLVC
                     FCSSGGARMQEALYSLLQMAKTAAALSRLAQAHLPFISVMTDPTTGGVSASLAMLGDL
                     NIAEPRALIGFAGPRVIQQTVRETLPEGFQRSEFLLEHGALDMIVDRRDMRDRIAAAL
                     RLLLKLPPAAAA"
     gene            5800..7098
                     /gene="folC"
                     /locus_tag="VFB37_16855"
     CDS             5800..7098
                     /gene="folC"
                     /locus_tag="VFB37_16855"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF008101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZO24167.1"
                     /translation="MRTLAEWLALQESVHPKAIDMGLARVSRVAQTLGVAQPACPVIT
                     VGGTNGKGSTVAHLEALLRAAGASTGMFTSPHFVRYNERIRIDGTEVQDDALLAAFER
                     IEQARGAITLTFFEYNTLAALLIFAERRVQVAILEVGLGGRLDATNLVDANVAIVCSI
                     GSDHRDWLGESLEQIGAEKAGIFRPGRPAVLSTAEVPASVYSIIEQLGARPVIAGRDY
                     RWELKGAAHRGEGTPAGADWSYQGIRLRAPDLPPSALAGSIQYRNAAAAIAAIESLES
                     RFVLSERSVQEGLRQVRLAGRFQVMQREGVEWILDIAHNEPAALVFAAQVRERRPPPS
                     GRTFAIVGILSDKDAPEIVAALEPLVSDWVLCGLPGPRGGSAADLARRVSVPLARVVQ
                     ADSVQEGCEKVCKAAKPGDRILVFGSVYTVGPVLQWLRIY"
     gene            7149..7799
                     /locus_tag="VFB37_16860"
     CDS             7149..7799
                     /locus_tag="VFB37_16860"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZO24168.1"
                     /translation="MKERLTGAVILVALIVLLVPELLTGPVRPSPSVGASSSSEEPPL
                     RSYTISLSDEARSKSQGASSSGPAMPQPGNTEPPGVAQPGESAQPGGSAQAGVSAQPM
                     TSALPSPPVSVNPAPTPPPAQKSSPPHNPKPAHTSAAGTGWVVQLGVFASRENAEHLA
                     QQMKSRGFKASVSESGGSSRRLYRVRIGPAPDRASAQTLQGKLRAAGHPGTLVPLS"
     gene            7911..8402
                     /locus_tag="VFB37_16865"
     CDS             7911..8402
                     /locus_tag="VFB37_16865"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZO24169.1"
                     /translation="MNATDYIVIAAVVISSFIGALRGFLREAISLGTWIIALFVAWHF
                     SDLIAPHLGGLMAGSAVRPWAARVIIVLVLLLLGSVIGVTLSHFVRLSIFSGMDRLLG
                     YVFGLVRGLVLFGVFVILAQTLRLDGERWWSHSLLMPYGESLANGLRVMMGAPRVPHS
                     HEV"
     gene            8437..9951
                     /gene="purF"
                     /locus_tag="VFB37_16870"
     CDS             8437..9951
                     /gene="purF"
                     /locus_tag="VFB37_16870"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZO24170.1"
                     /translation="MCGIVGIVGTSAVNQRLYDALTVLQHRGQDAAGIATAVDGELYT
                     RKGSGLVRDVFQQQHMLELRGNVGIGHVRYPTAGCDTASEAQPFYVNAPYGICLGHNG
                     NLTNAAELAEVLIREDRRHLNTTSDSEVLLNVFASELQRIGTPRVTPADVFAALAAVY
                     RRCRGGYAVVAMVIGHGILGFRDPHGIRPLVLGHRSTSRGPEWMLASESVALDMLSFK
                     FVRDIGPGEAVFIDELGRLHSQQLVPTVRHTPCIFEYVYFARPDSKIDNISVYRARMR
                     MGDRLADKILRERPEHDIDVVIPIPDTSRTSALQLAQRLGLKYREGFIKNRYIGRTFI
                     MPGQEQREKSVRGKLNAIDLEFRGKNVLLVDDSIVRGTTSAQIIELAREAGARKVYFA
                     SAAPPVRFPNVYGIDMPAVSELVAAHRSIEEVAHLIGADWLVYQDLEDLVAACRHDDA
                     RIVEFDTSCFSGEYVTGDVTPEYLERLQIERSDTAKTLRRGRRGKLKVIGQTAV"
     gene            9948..11165
                     /locus_tag="VFB37_16875"
     CDS             9948..11165
                     /locus_tag="VFB37_16875"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HZO24171.1"
                     /translation="MSSADPRRRLLLELFQAGVARVAGRGCVAGALRANGMQAGAVWI
                     AAIGKAAAEMALGAHDVLGAAITRVLVITKEGHVPPEARALPAVQIQESAHPVPDTRS
                     LEAGQKLLHWLRELPDSAQPLFLISGGASSLVEVLEVGVTLEQLGRLNAEGHASGIAI
                     AELNARRGRLSRIKGGRLAALLAGRPGRALFISDVPGDDPAVIGSGIMGPANAGPDHI
                     VRTIVASLEQAMEAACVSAEKMGLDVQRAPKRFDDDALRLAVRFSHELHLSPAQVCIW
                     GGESTVRLPERPGHGGRNQQLALAAARQLAGHAEDVLLAAGTDGTDGPTEDAGAIVDG
                     DTCARIAVAELDADDCLRRADAGTALAASGDLIHTGPTGTNVGDLVMGLKLSRQAAQS
                     IWQHRDARGARVL"
     gene            11172..12428
                     /locus_tag="VFB37_16880"
     CDS             11172..12428
                     /locus_tag="VFB37_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO24172.1"
                     /translation="MRLLLIEEDPRRCALIQHHLTSWKPDVGLVLHSPRLQGTLAPEF
                     LAQGFDAVLLSRLWAGGRGLDWVRDLASRTGFAPLVFLSEHSDDADSRRALELGAEAV
                     VGRDELDSQRFFEILETAAEQQTRARTARRKGAAGRESQSFGSAFIPGYRRVKQIAAG
                     PVSDLFLAESEAAGRLVVLKVARDQHQHQSDLDHTFRRFLQEYEIVQRIRHPSVVRLY
                     DLGVSDDHAWLVMEYFRAGDLRKRMRAGMRPRPALRTAIGIARALEAMHSAGVLHRDI
                     KPGNVMLREDGSLALIDFGMSKDTALDLDVTDSGMIFGTPHYMSPEQGHGEPIDGRSD
                     LYSLGVIVFEMLAREKPYAAENPMAIIYKHRKEPVPRLPPRFEALQPLIDRFLAKDPG
                     DRYANAREAAVALERAHRLLRGERTS"
     gene            12425..13039
                     /gene="miaE"
                     /locus_tag="VFB37_16885"
     CDS             12425..13039
                     /gene="miaE"
                     /locus_tag="VFB37_16885"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HZO24173.1"
                     /translation="MILLRPTPAAWLQSALERWREMLIDHASCEKKAASTALALMFTY
                     PEDEALSLALARLAREELRHFEQVQKMMRRLGVDFERQQPGRYALSLRSRLRTAEPGR
                     KLDLLLAGALIEARSAERFAMLSPHLHGPLADLYAQLERAEARHFEVYLELAQAVAPD
                     QWGARLAELAEWESELATAPDTLLRFHSGPIGADLTERCDRTRA"
     gene            complement(13008..13844)
                     /locus_tag="VFB37_16890"
     CDS             complement(13008..13844)
                     /locus_tag="VFB37_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24174.1"
                     /translation="MSTRKRSGALILLAWGALWAACLAGCASDRLVASAPGGVNLTGN
                     WKLDLNLSDDPDRLLDQDKSLSKPASSGRHHGHQNPGSDTGLPPMGTPDGPVPRFAGS
                     SAPLDARSFEADGISGPTARESSLSEQWTGGPSPAPAPGIEQSNPLPPSNTPSEADTS
                     ANRGRSVLARVLAAPSLLVITQDGPKVSIRSRMADGTGSADEFVAGTKAKVPYGKDST
                     AERESGWRGPVFVTTTKTNSGWREDDYALDEDGRLIVTSDIKAGHLKQLEIKHVYDRI
                     AP"
     gene            14029..14748
                     /locus_tag="VFB37_16895"
     CDS             14029..14748
                     /locus_tag="VFB37_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO24175.1"
                     /translation="MSDRPHLVIVDDDREIRTLLSQYLEKHDFRTTAVAEGKEMRRVM
                     ERSRVDLVVLDLMLPGEDGLAICRELRTRSQVPIIMLTARGEDVDRIVGLELGADDYV
                     PKPFNPRELLGRIRAVLRRAAHAPRDPSPDTVRSFTFNGWKLDTTTRTLTDAEGKEVA
                     LSGAEYRLLAVLLSSANRVLSRAQLTELLRGREADPFDRSIDVRVSRLRQILGDDARA
                     PQIIKTVYGEGYVVGVAVETH"
     gene            14773..16254
                     /locus_tag="VFB37_16900"
     CDS             14773..16254
                     /locus_tag="VFB37_16900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO24176.1"
                     /translation="MRLSLWPQSLFGRLLAASVLALLLAEAASLVLVARERERFVLQG
                     NVREWSRRIAEVTTMLQGLQGAERDAAAQRLTAGPLHFGRRADGVRLRSEADEDGEPP
                     RRGARDTRGSPSPGGRFDRSTASLPFVPFATQSEFEHTFAEQLQTTLGRDFRLSVSPT
                     TDPSRRAISITGTAFAENRDGPVELYDATVGFPDGYTAVFRVGRVSRGPPLPRGLFLN
                     LILLIVVMLVALFVTARSITHPLSELARAADRVGRDLRQPRLAERGARELRHAARAFN
                     TMQDRLQRYLDSRSRVLAAMSHDLKTPLTRLRLQVETLEDPVIQARFGKQLDEMESMV
                     HGALALFRGLDDNEAFAPIDMDALLRTLQSEFAEMNASVILQGRALRPISAKPQALRR
                     CLTNLIANAVKFGSRATIVIEDGQALILRIRDEGPGIPEEELERVFEPFYRLETSRNR
                     DTGGTGLGLSIARDVAQAHGGSLVLHNLPVRGLEAVLTLPRIS"
     gene            complement(16281..17003)
                     /locus_tag="VFB37_16905"
     CDS             complement(16281..17003)
                     /locus_tag="VFB37_16905"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZO24177.1"
                     /translation="MSRLFVSDVHLDAGTPDAVEQFLSFLRTQAAAAQALYILGDLFE
                     TWVGDDDADPAKERVRAGLRALTASGVGCFLIHGNRDFLLGHDFCRRTGARLLTDPTI
                     ADLEGERVLLTHGDTLCTDDHSYQELRSIVRSAEWQRRFLSLPLADRELLANEARAGS
                     REHILRTVPYIMDVNPDAVATAFRATGVRRIIHGHTHRPGVHDTSVDGKPAQRLVLGA
                     WYEQGSYLIHERGQYALRELPR"
     gene            complement(17022..17855)
                     /locus_tag="VFB37_16910"
     CDS             complement(17022..17855)
                     /locus_tag="VFB37_16910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO24178.1"
                     /translation="MLRDTALRRDFQLLRHMDVAIRTSIPAEAHGGSMRILPWYRPCT
                     PAATALLLFAGLLLQGPVRAADPPAQPQPPAAPAAAPQSPPQVKVTTSMGEFVIELMP
                     DRAPLTVANFLRYVHEGFYSGTLFHRVIANFVIQGGGHSATDYKLKPVHDAVVNESGN
                     GLQNKRGMVGLARGESPHSGNAQIYVNLVDNPDLDPLPTRWGYTVFGKVIQGMDVVDR
                     IGVTPTGAMGPFKSDAPLKPVVIEKIELIDAGSPTNGPVAAPAPTAVPPASPDTILSP
                     K"
     gene            17862..19301
                     /gene="gltX"
                     /locus_tag="VFB37_16915"
     CDS             17862..19301
                     /gene="gltX"
                     /locus_tag="VFB37_16915"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZO24179.1"
                     /translation="MSTMDTPAGESFGAGGPVTRFAPSPTGALHLGNARTALFNYLLA
                     RRGGGRFVLRIEDTDVQRSHEDHTQGLIQDLHWLGLEWDAGPDREDSLGPYRQSERTA
                     LYERYFAELEAAGAVYPCFCSALELEASRRAQLAAGRPPRYAGTCRELTPAEQASRRA
                     AGALASLRFRVPLGRRIEFTDFVHGPQSFLSDDIGDFVVRRTAGTAAFFFCNAVDDAR
                     MGVTQVLRGEDHLTNTPRQLMILEALSLSAPVYGHISLLVGNDGAPLSKRHGATSVRQ
                     YRDRGYLSEAMTNHLFRLGHSTAEHGFLTLEQMVRAFDVNHLGRAPSHFDEQQLNVWQ
                     KEGAHRLSRDAALRWLEPVLPQGIDERLRHEFVAAILPNLVLPEDARVWVEVVFGKPP
                     DLEAKDEQVVREAGRGYFSAAAAAAAASGNDLPAIAGAVRAATGRKGAALYMPLRVAL
                     TGRAHGPELAPLVRAMPAGQARERLARFA"
     gene            19333..20769
                     /gene="cysS"
                     /locus_tag="VFB37_16920"
     CDS             19333..20769
                     /gene="cysS"
                     /locus_tag="VFB37_16920"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZO24180.1"
                     /translation="MVPIRIHDSMTGEKQLLQPITPGHVRMYVCGMTVYDYLHIGHAR
                     MLTVFDLVSRYLRHRGYRVTFVRNITDIDDKIIKRAAEGNEPIEAVTERFIRAMHEDC
                     QALGLTSPEFEPRATQYLPGIIDMISRLCERGYAYSAPDGDVLYAVAKFEGYGRLSGK
                     RLADLRAGARVEVDEGKRDPLDFVLWKHAKPGEPFWESPWGRGRPGWHIECSAMSTAL
                     LGTHFDLHGGGMDLKFPHHENEIAQSCAATGDRFANLWMHNGFVNIDNEKMSKSLGNF
                     FTVREILPRLRHPEVLRYFLLSSHYRGPINYSLDQLDQADLALGRIYTALREVPPGPV
                     LASAHTAGFHAVMDDDLNTPEALAALQNLTRELNSAKAAGESDRAAALAAELRSLAGV
                     LGLAQLQPQDWFRRASPAAQGSVGGAGSGARAEAFSDSDIESRIEARNAARAARNWAE
                     SDRIRDELSRAGIVLEDKPGGRTTWRRA"
     gene            complement(20779..21780)
                     /locus_tag="VFB37_16925"
     CDS             complement(20779..21780)
                     /locus_tag="VFB37_16925"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZO24181.1"
                     /translation="MHITLSFTALAMLVALAVAFDFLNGFHDAANSIATVVSTRVLRP
                     YQAVAWAAFFNFVAFAIFKLSVAATIGKGILAPEFVDARVIASALAGAILWDLATWYY
                     GLPTSSSHALIGGMIGAGMARAGTAPLIMTGILKTVAFMVISPLLGVLLGAAFLVAIS
                     WICFHRSPYMVDRWFRRLQLLSSALYSIGHGSNDAQKTMGLIWLMLISAGMTSRDHLP
                     FWVVASCYAAMALGTLFGGWRIVRTMGQRITKLRPVGGFSAEMGGALTLFLASALGIP
                     VSTTHTITGAIVGVGAAQRVSAVRWGVAGDIVLAWIITLPAAALIAAGTWWLARLIG"
     gene            complement(21808..22452)
                     /locus_tag="VFB37_16930"
     CDS             complement(21808..22452)
                     /locus_tag="VFB37_16930"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZO24182.1"
                     /translation="MQFLSRLMPRERRFFELFDQHAALVVAAGAVLAELLRGFTHEAD
                     KRDARIAEILELEHRADKIAHETFGLLHRTFVTPLDRDDIHLLVSRLDDVLDVIQDAA
                     ESFQLYDVRQLTPQACELGDLVHECCLRLQKAVHLLSSTGESARTLELCTEIDGLESQ
                     ADRTMRAALSKLFREEGDVRQLIKLETVYQLLESATDRCEDVANIIQGIVLENA"
     gene            22666..22968
                     /locus_tag="VFB37_16935"
     CDS             22666..22968
                     /locus_tag="VFB37_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24183.1"
                     /translation="MHLRLRAPIVLAALAILAAGGCAAPEHRMNVAASRDDTRRADFE
                     RCRQEGRTDCDAILNAPVNSNTPPRGDSVREQERREAYDRCVARGGSDCEDLLHHD"
     gene            23099..23175
                     /locus_tag="VFB37_16940"
     tRNA            23099..23175
                     /locus_tag="VFB37_16940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23133..23135,aa:Pro,seq:tgg)
     gene            24169..25650
                     /locus_tag="VFB37_16945"
     CDS             24169..25650
                     /locus_tag="VFB37_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZO24184.1"
                     /translation="MSRTDTARLRELESLYAALNNSQAVIELDMKGTILAANDNYLKL
                     FGYTRAESLGQPYSLFVNPADREGPEYKALWDSLRSGRYQQGRFRRMGKNGREVWVRA
                     SFNPLIDRDGRPWKVIKLAAEITHIIVEGRALDYAVQESQEVIQSALKGTGDRRISLN
                     GKTGHLELLAAGVNELIDGVQKTVAETMEVVQRAVDGDLTSRVRVDDKSGHFRTLAVS
                     VNSMIEFMMDVVTTLTEASHEVQVSAEEISRGNANLGRRTAEQASSLEETASSMEEMT
                     TTVKKNAEHAVQASQLAEAAREQAERGNRIVGSAVAAMGEINTASKKIADIIGVIDEI
                     AFQTNLLALNAAVEAARAGEQGRGFAVVATEVRTLASRSAAAAKEIKRLIQDSVGKVD
                     EGTKLADESGAALSDIVRRVKSVTAVMTEIAGASREQASGIEQVNRAVTLMDNMTQQN
                     AAMVEEASAAVQRLIEQATALTRLIARYCKSESRPSALIPNVR"
     gene            25927..26000
                     /locus_tag="VFB37_16950"
     tRNA            25927..26000
                     /locus_tag="VFB37_16950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25961..25963,aa:Arg,seq:tct)
     gene            26150..26225
                     /locus_tag="VFB37_16955"
     tRNA            26150..26225
                     /locus_tag="VFB37_16955"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26183..26185,aa:His,seq:gtg)
     gene            26325..26400
                     /locus_tag="VFB37_16960"
     tRNA            26325..26400
                     /locus_tag="VFB37_16960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26358..26360,aa:Lys,seq:ctt)
     gene            complement(26438..27358)
                     /locus_tag="VFB37_16965"
     CDS             complement(26438..27358)
                     /locus_tag="VFB37_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24185.1"
                     /translation="MGKLACGLSIPGVFEKDGRYYKVVRNRWIALSRIDEGINSLYRA
                     LYELDPARPGTVGEMIAVYRAAGMDNLRPSTQARYGLMLTRLDKTFGKMRIGTLRPSH
                     VAMHLEKRRKRGRGAIAANREMAVLASVHNFGMRQGWLEANPCRGIARNTEKPRKRNV
                     TDAEFLEAFHASPEPFQDLIAAAYLSGCRQGDVVSWTRSGNLKPEGIVFVESKTGKPH
                     TVEWSDALRFFVRRAMARFPDTDHVFANRFGQPWSVWAINSQMRRLKVTWAFRDLRAK
                     AQTDSPHSVLGHGAAMEAVYRKMLRTKPVR"
     gene            complement(27546..28421)
                     /locus_tag="VFB37_16970"
     CDS             complement(27546..28421)
                     /locus_tag="VFB37_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24186.1"
                     /translation="MPAAYPHRKGVSRCNSCIILNSNSFGELMSLTLPAVIRHEISKL
                     PPTERDIVAQQLRHAPSPPIVYEEAVKQLASVRDIDTTKRVLLAAEGLALWGRLHEDN
                     RAIVEAQKLKAHALRQMFICAKMLGEPYKVLAERRIWSSKAKRGEELASFEDKSEIDR
                     LADEVSVSGRAEFLVGRIADESPLAKSVREANEKSWQEAQAKQRQADERAKQREKQEA
                     AKRDTPQQRFFDHALSPLIDVEQILEKVHEFVVTHASKANKKHVVERVTNIMELLDKI
                     DAACQPRSAGRPKRA"
     gene            complement(28510..28884)
                     /locus_tag="VFB37_16975"
     CDS             complement(28510..28884)
                     /locus_tag="VFB37_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24187.1"
                     /translation="MSARVRTECGLAYLSEAYTEMRGEVLDVKEYAAGYFIDGDVASR
                     DFLREIALLHREQLPEGRMTSLRRHRDALAELVGLITLVCIRGDLPDEIRDVLLSNWR
                     TVQACRLLAPVCLEPEPAEKAA"
     gene            complement(29032..29388)
                     /locus_tag="VFB37_16980"
     CDS             complement(29032..29388)
                     /locus_tag="VFB37_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24188.1"
                     /translation="MMFFNRKLHQMLSLILQELQNVNATVDSLSNEITQLTAAVTAEQ
                     TVNQSAITLINGFAAQLQAAVAAATAAGATPDQLAALNGLQTQITTSTAALAAAVSAN
                     TPPPPPPTPAVAPPSA"
     gene            complement(29385..29747)
                     /locus_tag="VFB37_16985"
     CDS             complement(29385..29747)
                     /locus_tag="VFB37_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24189.1"
                     /translation="MSLVNFSEAAREQRAEWLDMRELCAQWFLESEEAARDILRGIAH
                     EHRRQHSGAMPVVVRHRAVLSDLVGLVQLISAREDLPGEIRAQIQNNFRFTNALALLN
                     RQAEQFVHSDAPPIVDAA"
BASE COUNT         5073 a         9589 c         9871 g         5282 t
ORIGIN      
        1 ggtggcggtg gtgggtgcga ccgggctggt cggtgagacc atgatcgagg ttctcaccga
       61 gcggaacttc ccggtgtcgg aactgtatcc gctcgccagc aaccgctcgc tcggcaagtc
      121 ggtggagttc cgcggccgca gctacccggt gggcgagctg gcggagttcg atttctccaa
      181 agcggacatc ggactgttct ccgccggagc ggaagtttcc cgggagtttg cgcctcaggc
      241 tgcagcggcc ggttgtgtcg tgatcgacaa cacctccgag ttccgttacg aggatgacat
      301 tccgctggtc gtgcccgaag tgaatccgca ggcgatcgcg cagtacacgc agcgcggcat
      361 catcgccaat cccaactgct cgaccatcca gctcgtgctc gcactcaagc cgctgcacga
      421 tgcggtcggc atcgagcgga tcgaagttgc aacctatcag tcggtctccg gcgcgggacg
      481 cgaggcactc gaggagctcg ccgcccagtc cattcagctg ctgagcggca aggggccggt
      541 gaccgcccgc gtggtggcca agcagattgc cttcaactgc gttccgcaga tcgatcgctt
      601 cgaggacaac ggctacacgc gcgaggaaat gaagatcgtc tgggaaacgc gcaagatcct
      661 cgatgacccg caaatcctcg tgaacgccac ggccgtccgg gtaccggttt tctacggcca
      721 ttccgaggtg gtgcacctgc aaacccggac gaagatcaca gccgcgcagg cgcgcgcgcg
      781 gctctcagag gcaccgggga tcgaggtcat ggatgagcgg cgcgcagggg ggtaccccac
      841 ggccgctacc gaggcagcga accgcgacac agtttatgtg gggagaatac gtgaagatcc
      901 gtcctctgag aggggactta acttatggat agtcgcggac aacgtgcgca agggcgctgc
      961 cacgaacagc gtgcagatcg cagagatttt ggctcggcag catatgtaat gtatattgcc
     1021 gcccgttcgg tgggcccaca aaaggcggcc cactcggttg tttttacaag atatttcacc
     1081 cactgatgcc tgtcggggtg tgccattcgg ggtgtcgtaa tggtcgctaa atcgctcggc
     1141 cgcacactgg ctttgctgat ggccctgcca tcggccgcct ttgccgtcgg tctcggagac
     1201 attcatctcc tgtcgccgct caatgccccg ctcgatgcgg agatcgagct gctggatgcc
     1261 acgcccgagg aaatgcagag cctcaaggcc cagctggcct cgcgcgacac ctttgcccgt
     1321 tacggtctgg attggccggt attcctgagc agcgtccagg tgcacaccgt gcgcaccccg
     1381 gacggcaagg agatgatcaa gctcaattcg accgacgccg tcaccgagcc cttcatcacg
     1441 ctgctggtgg aggtcaactg ggcacgtggt cgagtggtgc gcgagtacac gatgctgttg
     1501 gacccgcctg tctacacccc tggacagaac gcgggcgcca acgtgccggt tgcgacgccc
     1561 accgccggcg ccggcgtgcg tgaaggctcc atcgcccgaa agagcgacac tcagaccgct
     1621 gacgatccgg cgcccgcgac gccaacggcc ccaccgagca cctcggcctc caagtcggcc
     1681 cctcacgcgc aggcggtgag cagcggtgcc ggggatccgg cctcagcagg ctcgacccat
     1741 ctcattcaac gcggcgagac gctttcggcg attgctgcgt ccacggctgg aagcccggcc
     1801 aattcgcccc aggcgcgctc ctggatgctg gcgatctatc aggccaatcc gggggccttc
     1861 gacaaaaaca tgaacctgat gcgctcaggt gccgtgctgc gcattccgga cagcgcacaa
     1921 gtcgcctcca tttccgccag cgaagcgagt ggcgagatcc gccggcagta cgcggcatgg
     1981 cgctccacga ctccggcagc tcctgcagcc tcggcttccg agccggggcg gctgcgcctc
     2041 gtgaccccga gccagccggg cgcaggcggc gcgggcaatg gcgcagaggt ggaagccctg
     2101 cagggccgcg tcaaggatct cgaaggacaa ctgagcgatt cgaagcggct tctggagctg
     2161 cgcaacgcgg agctggcgaa attgcaggcg caactcgccg gcaagaacgc tcccgcgcca
     2221 acgcaacccg aggctgctcc ggcgcccccg gcagcggctc cctcgccgcc gcccagcgct
     2281 gcggcaccga caccgccccc ggtcgagcag ccggcggcga aggctgaaac accagccccc
     2341 gcgcccgtcg cgcaggagcc gccgcccgcg gccgagccga cacccgtgcc ggcgaagccg
     2401 gtcgttgcgc ataagccgac tccggcttcg caatcgcagg gcggctcgat tctcgattcg
     2461 ctgaaggatt tctggtgggc gatcgtgctg gtgctcgtgg cactcgccgc gttcatcggc
     2521 ctgcgcgcct ggcgctcgcg ccgtcaatcg gagttcgatg actcgcttgg ccgcctcgcg
     2581 gtggctggcg ccaactccat ggatgacggc ttcggcaccg gtgacacggc tcccgtgcgc
     2641 ccgctgtcag cggccagtgg cgaaggcgcc ttcctggtcg aggagtcagg cacacatcag
     2701 cggccgcgat tcccgggcgc agctccggcg gctccctcgg ccaagcacgt ctccaccgac
     2761 gagacgatca gcagcgagac cgccatcaac ctcgaccagg gagacccgct ggccgaggcc
     2821 gatttccaca tggcctacgg tctgtatgac caggccgccg acctgatccg catcgcgatc
     2881 agccgtgagc cggcgcgccg cgatctgaag ctcaagctcc tcgaagtgtt cttcgtctgg
     2941 ggcaacaagg atcagttcct gcagagtgcg cgcgagctgg ccaacacccg tgaccaggcg
     3001 gccccgggcg agtgggaaaa gatcgtcatc atgggcaagc agcttgcccc cgaggacccg
     3061 ctgttcgccg gtggcggcgc agtcagcggc gccaccgccg gcggggtgga tctggacctc
     3121 gaaggcggcg agagccgcgt cgacttcgat ctgctcggcg agccggtcgc aggccaggtc
     3181 cctggatccg tcgatctcga tatcggctcc gctctcggcg agcccgccga tgccggcacc
     3241 gattccgcga cgggtgcgac tgaccgcagc ttcgcgttgc agagcaacgc cgcgaccggc
     3301 accacgcggc agatgaccca gaagatcaac cgggatccgg aaccggtgct ccccgagttc
     3361 accaccgaga ccgagggccc gacgatcgag cagccgggtc tgagcactcc gcagaacccg
     3421 accatccgcc agaaggtcgc gatggcgctc aagcaggggc attcggccga gcagaccgcc
     3481 gagctcgcca ttgacgatct cggcctggat ctcggcgcgc tcgacacggt cgaccagccg
     3541 ggcctcggag cttctcccga tgcgccgacg ctcgtcgccg gcatggacga tcgctcccgc
     3601 aaggtgatcg aggaagccca gcgccgcgcg tctgcggaag accacgatac ggcggcaacg
     3661 gcagccttcc gcctcgatga tcaggacctg gaggcggcct tcggtgctcg cgcagccaac
     3721 ggccatggcg aaacgactgc cttcgatact tcttccactt cgcgcctctc gaccctgagc
     3781 agccgcgacg ttgacttcga cctgagtgag ggcgatgcgg gccacgtcgc ccatggaacc
     3841 aacggcgccg ggctcgatct ggatgtcggc accgccacgg taccggatgc ggccttcacc
     3901 tcgacccaga agctggcctc cgacgatctt gctttgccgg atctcgagcc ggtgacgatg
     3961 agcgaggtcg gtaccaagct cgatctggcc cgggcctaca tggacatggg tgatccggaa
     4021 ggcgcgcgca acatcctcga ggaggtgctg catgagggct cggtggcgca gaagcaggag
     4081 gcgcagcgcc tgatcgagtc gctcccgggt tgaggctggc tgtcgggatc gagtacgacg
     4141 gagccgccta cgcgggctgg cagagacagc ccggcctgca cacgatccag gcgcatctcg
     4201 agagtgctct cagtagcgtt gcggccgagc ctatcgggct cacaggcgcc ggacggaccg
     4261 atgccggcgt gcatgcgcgc tggcaggtag cgcacttcga gacgcagtcc agccgcccga
     4321 tgcgaggttg gcttctgggc gcgaataccg agcttccgcg cgacatcagt atcctgtggg
     4381 cgactccggt gccggcgcac tttcatgcgc gctactccgc cgaggcacgc acctaccgct
     4441 acttcatcct caatcgcggc gcccgcccgg cgcttgcggc gaagcgggcc acctggatcc
     4501 acaagtcgct cgaccatgag cgcatggcgg aggccgccgg tctgctgcga ggcgagcacg
     4561 acttcagcgc attccgctcc tctgaatgcc agtcgcgctc gccggttcgc cgcctgcagg
     4621 agctgatcgt acggcgcgag ggcgagtggg tggtgatcga ggcgaccgcc aatgccttcc
     4681 tgcaccacat gatgcgcaac atcgtgggcc tgctcacgaa ggtgggccgt ggcgatgccc
     4741 cgcccgcctg ggcgggggaa gtactggcgg gtcgcgaccg cacgcgggca gcgccgaccg
     4801 caccgcccga tgggctgtac ctctgggcaa tccgctaccc gaatgccttt gctctccccg
     4861 agccgggagc gggggctgcc tcaggcgccc gatccgctat gatcctcgga ccaccctgaa
     4921 cggacactgc catgtcgtgg ttcgagaaga tcatgccctc gcgcatcaag accgagcggc
     4981 gcacgcgctc ggttcccgag ggcctctgga tcaaatgccc ggcctgcgat gcggtgctct
     5041 atcgagccga gctcgagcgc aatctgtatg tctgtcccaa gtgcagccat cacatgcgaa
     5101 tcggtgcccg cgagcgtctg gagagcttcc tcgaccccgg cgaaacggag gagatcggga
     5161 ccaggatctc gccggaagat ccgctgaagt tccgcgacag caagcgctac aaggatcggc
     5221 tcacgcaggc acagaaggcg acgggtgagc gcgatgcgct catcgccttg agcggcaagc
     5281 tgcaggatct gccgatcgtg gcctgtgcgt tcgaattcca gtttctcggc ggctccatgg
     5341 gatcggtggt cggcgagcgc ttcaaacgcg cggtcgatcg ctgtatcgag aagcgccagc
     5401 cgctggtgtg cttctgctcg agcggcgggg cgcgtatgca ggaggcgctg tattccctgt
     5461 tgcagatggc caagaccgcg gcggctctct cgcgtctggc gcaggctcat ctgcccttca
     5521 tctcggtgat gaccgatccg accaccgggg gggtctcggc cagcctggcc atgctcggcg
     5581 atctcaacat cgcagagccg cgcgcgctca tcggcttcgc cgggccccgt gtcattcagc
     5641 agacggtgcg tgagacgctg cccgaaggct tccagcgcag cgagttcctc ctcgagcatg
     5701 gggccctgga catgatcgtc gaccggcgcg acatgcgcga ccggatcgcc gccgcgttgc
     5761 ggctgctgct gaagctgcca ccggccgcag ctgcctgact tgcgcacgct ggcggaatgg
     5821 ctggctcttc aggagtcggt gcatccaaaa gccatcgaca tgggccttgc gcgtgtctcg
     5881 cgcgtcgccc agacattggg tgtggcccag ccggcttgcc ccgtcatcac agtggggggg
     5941 acaaacggca agggctccac ggtggcgcat ctcgaggcgc tcctgcgcgc cgccggggcg
     6001 agcaccggaa tgttcacctc cccgcatttc gtgcgctaca acgaacgaat tcgcatcgac
     6061 ggcacggaag tgcaggacga tgcgctgctg gcagcgttcg agcgcatcga gcaggcgcgc
     6121 ggtgcgatca cgctgacctt cttcgaatac aacacgctgg ccgcactgct gatcttcgcc
     6181 gagcggcgcg tgcaggtggc catactcgaa gtcggcttgg gcggccgcct ggatgcgacc
     6241 aatctggtgg atgcgaacgt cgccatcgtc tgctcgatcg gctcggacca ccgcgactgg
     6301 ctcggcgaga gcctcgagca gatcggtgcg gagaaggccg gcatcttccg tcccgggcgc
     6361 cccgcggtcc tgagcaccgc ggaagtcccc gccagcgtgt actcgatcat cgagcagctc
     6421 ggtgcgcggc ccgtcattgc gggccgcgac taccgttggg agctcaaggg tgccgctcac
     6481 cggggtgagg gcacgcctgc gggggcggat tggagctacc agggaatccg gctgcgtgcg
     6541 ccggatctgc cgccttcggc actggccggc tcgattcagt atcgcaatgc ggccgcggcg
     6601 atcgccgcca tcgaatcgct cgagtcccgc tttgtgctga gcgagcgaag cgtgcaggag
     6661 ggactgcgcc aggtgcggct cgcgggccgc ttccaggtga tgcagcgcga gggcgtggaa
     6721 tggattctcg atatcgccca caacgagcct gccgcgctcg tgtttgcagc acaggtgcgc
     6781 gagcgtcgcc ccccaccttc ggggcgcact tttgcgatcg tcggcatcct gagtgacaag
     6841 gacgcgccgg aaatcgtggc tgcgctcgag ccgctggtga gcgattgggt cctgtgcggg
     6901 ctgcccgggc cccgtggcgg ctcggcagcg gatctggctc gccgggtgag cgtgcccctg
     6961 gcgcgggttg tccaagcgga ctccgtacag gaaggctgcg aaaaggtgtg caaagccgcc
     7021 aaacccggag atcgcattct cgtgttcggc tccgtctaca ccgtaggtcc ggtgctgcag
     7081 tggcttcgga tatactgagg tgtcctcggc ttgattcacg cgatgacgcc cccattgtga
     7141 gccaactggt gaaagagcgg ctgaccggcg cggtcatcct ggtcgcgctc atcgtactgt
     7201 tggtgcctga gctgttgacg ggaccggtgc gtccctcgcc ttcggtcggc gcgagcagtt
     7261 cctccgagga gccgccatta cggtcctaca cgatcagcct gtcggacgaa gcccgctcga
     7321 aatcacaagg tgcgagcagc agcggaccgg cgatgcccca acccggcaac acagagccgc
     7381 cgggcgtcgc gcagcccggc gaatccgcgc agccgggcgg atccgcacag gcgggcgtct
     7441 ccgcgcagcc catgacgagc gcgctcccct ccccgccggt gagtgtgaat ccggcaccaa
     7501 caccgccgcc ggcacagaag tcatcgccgc cgcacaaccc caagcccgcg cacaccagcg
     7561 ccgccgggac cggatgggtg gtgcagctgg gcgttttcgc aagtcgcgag aacgccgagc
     7621 atctggcgca gcagatgaag agccggggct tcaaggcgtc cgtctcggag tcgggtggct
     7681 cgagccgccg gctctaccgg gtgaggatcg ggcccgcacc cgatcgcgcc tcggcccaga
     7741 cgctgcaggg caaactgcgg gcggccggtc accccggaac gctcgtcccg ctttcctagc
     7801 ccgtgacagc cgttcgcgca gcctgtccgc ccctcttgta caatccgcgc cgcgacaaag
     7861 gaccgtgacc gcaccaggtt ccatctcacc ggggttgaca ggctgcgatg atgaatgcaa
     7921 ccgactacat tgtgatcgcc gcggtggtca tctcgtcttt catcggggcg ctgcgcggct
     7981 ttctgcgcga agccatctcg ctcggcacct ggatcattgc gctgttcgtc gcgtggcact
     8041 tctcggatct catcgcgccg catctgggcg gcctgatggc cggctcggcg gtgcgaccct
     8101 gggccgcgcg tgtcattatc gtgctcgtgc tgctgctgct cggctcggtc atcggtgtga
     8161 ccctgtcgca tttcgtgcga ctgtcgatct tcagcggaat ggatcggctg ctgggttatg
     8221 tcttcgggct ggtgcgcggg ctggtgctct tcggcgtgtt cgtgattctc gcgcagacgc
     8281 tgcggctgga tggggagcgc tggtggagtc attccctgct gatgccgtac ggggaatcat
     8341 tggcaaacgg gctgcgggtg atgatgggcg ccccgcgcgt gccgcattcg cacgaggtct
     8401 gagcgccggc aagtcacaac gcgaggtcgg cgaagcatgt gtggaatcgt aggcatcgtc
     8461 gggacgagcg ccgtcaacca gcgcctctat gatgcgctga cggtcctgca gcatcggggg
     8521 caggacgctg cgggtatcgc cactgcggtg gatggcgagc tctacacgcg caagggcagc
     8581 ggtctggtgc gcgatgtctt ccagcagcag cacatgctag agctgcgggg caatgtcggg
     8641 atcggccatg tccgctatcc gaccgccggc tgcgataccg cctccgaggc gcagcccttc
     8701 tacgtgaatg cgccttacgg catctgcctc ggtcacaacg gcaatctcac caacgcggca
     8761 gagcttgccg aagtgctcat ccgggaagac cgccgccacc tcaacacgac ttccgactcg
     8821 gaagtgctcc tgaacgtgtt tgcttcggag ctgcagcgca tcggaacgcc gcgcgttacc
     8881 cccgcggacg tcttcgcggc cctggcggcg gtctatcggc gctgtcgagg cggttatgcc
     8941 gtggtggcga tggtcatcgg gcacggcatt ctcggctttc gcgatccaca tggcatccgc
     9001 ccgttggtgc ttgggcacag atccacctcg cgcggtccgg agtggatgct ggcttccgaa
     9061 agcgttgcgc tcgacatgct ctccttcaag ttcgtgcgcg acatcgggcc gggcgaggcc
     9121 gtgttcatcg acgagctcgg gcgcctgcat tcccagcagc tcgtgcccac cgtacggcac
     9181 accccctgta tcttcgaata tgtctatttc gcgcggccgg actcgaagat cgacaacatc
     9241 tcggtatatc gcgcgcgcat gcgcatgggc gatcggctcg ccgacaagat cctgcgcgag
     9301 cgccccgagc acgacatcga cgtcgtgatt ccgattccgg acaccagccg caccagcgca
     9361 ctgcagctcg cgcagcggct cggcctgaag tatcgcgaag gcttcatcaa gaatcgctac
     9421 atcggccgca ccttcatcat gccgggccag gagcagcgtg agaaatccgt ccgcggcaag
     9481 ctcaacgcga tcgacctgga gttccgcggc aagaacgtgc tgctggtcga tgactcgatc
     9541 gtgcgcggga ccacctccgc acagatcatc gagctggcgc gcgaggccgg cgcccgcaag
     9601 gtctatttcg cctccgcggc gcccccggta cgcttcccca atgtctatgg catcgatatg
     9661 ccggcggtga gcgagctcgt cgccgcccac cgcagcatcg aggaggtggc tcatctcatc
     9721 ggtgcggact ggctcgtcta tcaggacctc gaggacctgg tcgccgcctg caggcacgat
     9781 gatgcgcgga tcgtcgaatt cgacacgtcc tgcttctcgg gcgaatacgt caccggggat
     9841 gtgacgcccg aatatctcga gcgcctgcag atcgagcgtt cggatacggc caagacgctg
     9901 cgtcgcggcc gtcgcggcaa gctcaaagtc ataggccaga ctgccgtttg agctccgcag
     9961 atcctcgccg ccggctgctg ctcgagctct ttcaggcggg cgtggcgcga gtcgcgggtc
    10021 gcggttgtgt ggcgggcgct ttgcgcgcga acgggatgca ggccggcgcg gtctggatcg
    10081 cagccatcgg caaggccgcc gccgagatgg ctctcggcgc gcacgatgtc ctcggcgccg
    10141 ccatcacccg cgtgctggtg atcaccaagg aaggccatgt gccgccggag gcgcgggcgc
    10201 tgcctgcggt gcagattcag gagagcgctc acccggtgcc ggatacgcgc tcgctggaag
    10261 cggggcagaa gctattgcac tggctgcggg agttgcccga ctcggcccag cccctgtttc
    10321 tgatctccgg cggggcgtcg agcctggtcg aggtgctcga ggtgggggtt accctcgagc
    10381 agcttggaag gctcaacgcg gagggtcacg cgagcgggat tgcgatcgcc gagctcaatg
    10441 cgcgccgggg ccggctgtcg cgcatcaagg gtggacgact cgccgcgctg ctcgccggcc
    10501 gcccggggcg ggcccttttc atatcggacg tgcctgggga tgatccggcc gtgatcggct
    10561 cgggcatcat gggcccggcg aatgccggac ccgaccacat cgtgcgcacc attgtcgctt
    10621 cgctcgagca ggccatggaa gccgcctgtg tgagcgccga aaaaatgggt ctcgatgtcc
    10681 agcgtgcgcc gaagcgattc gatgatgatg cgctgcggct cgccgtgcgc ttttcccacg
    10741 agctgcactt gagccctgcg caggtgtgca tctggggcgg ggagtccacg gtgcgccttc
    10801 ccgagcggcc cggtcatggc ggtcgcaacc agcagctcgc cctcgccgcg gcacgccagc
    10861 tggccggtca tgccgaggac gtgctgctcg cagccggtac tgatggaacc gacgggccga
    10921 ccgaggatgc aggtgcgatc gtcgatggag acacctgtgc ccgcattgcg gtcgcggagt
    10981 tggatgccga tgattgcctg cggcgcgccg atgccggcac tgcgctggct gccagcggcg
    11041 acctgattca caccggcccg accggcacca acgtcggtga tctggtcatg ggcctgaaac
    11101 tctcgcggca agctgcccag agcatctggc agcaccgtga cgcccgcggc gcgcgcgtgc
    11161 tgtaatgact catgcgtctg ttgctgattg aagaggatcc gcgccgctgc gcgctgattc
    11221 aacatcatct gacaagctgg aagccggacg tgggactggt gctgcacagc ccacggttgc
    11281 aaggcacact cgcgcccgag ttcctggcgc agggtttcga tgcggtgctg ctgagtcgcc
    11341 tctgggcggg gggccgggga ctcgattggg tgcgcgatct cgccagccgc accggcttcg
    11401 ccccgctggt gttcctcagc gagcactccg acgatgccga ttcccgccgg gctctggagc
    11461 ttggggccga ggcggtcgtc gggcgcgatg agctcgacag ccagcggttt ttcgaaattc
    11521 tggagacggc cgcggagcag cagacgcgcg cccgcaccgc ccggcgcaag ggtgcggccg
    11581 ggcgcgaatc gcagagcttc ggcagtgctt tcattcccgg ctatcggcgc gtcaaacaga
    11641 tcgccgccgg cccggtgtcg gatctgtttc ttgccgagag cgaggcggct ggccgcctgg
    11701 tcgtgctgaa ggtggcgcgt gatcagcacc agcaccagag cgacctggat cacacctttc
    11761 gccgctttct gcaggaatac gagatcgtgc agcgcatccg gcatccgagc gtggtgcgcc
    11821 tttatgacct cggcgtgagc gatgatcatg cgtggctggt gatggagtat ttccgtgcgg
    11881 gcgatctgcg caagcgcatg cgcgcgggca tgcggccgcg accggcgttg cgcaccgcca
    11941 tcggcatcgc ccgggcgctg gaggctatgc actccgcggg cgtgttgcat cgggatatca
    12001 agcccggcaa tgtcatgttg cgcgaggacg gctcactcgc gctgatcgat ttcggcatgt
    12061 cgaaggatac ggcgctggat ctcgatgtca ccgactcggg catgatcttc ggtacgccgc
    12121 actacatgag tccggagcag gggcatggcg agcccatcga tgggcgcagc gacctgtaca
    12181 gcctcggcgt gatcgtcttc gagatgcttg cgcgcgagaa gccctatgcc gccgagaatc
    12241 cgatggcgat catctacaag caccgcaagg agccggtgcc acgtctgccg ccccgcttcg
    12301 aggccctgca gccgctcatc gaccgcttcc tggcgaagga tccgggcgat cgctacgcca
    12361 atgcgcgtga ggcggccgtg gccctggaga gggcgcaccg cttactgcga ggggagcgaa
    12421 cgagttgatc ctgcttcgcc cgaccccggc agcgtggctg cagagcgccc tcgagcgctg
    12481 gcgggagatg ctcatcgatc atgccagctg cgagaagaaa gcggcctcca cggcgcttgc
    12541 gctgatgttc acctatcctg aggatgaggc gctttcgctg gcgctggcgc gtctggctcg
    12601 agaggagctg cgtcacttcg agcaggtgca aaagatgatg cgcaggcttg gggtcgactt
    12661 cgaacgccag cagccgggtc gctatgccct ctctctacgc tcgcggttgc gcacggccga
    12721 accgggtcga aagctcgacc tgctgctggc aggggcgctc atcgaagcgc gttccgcgga
    12781 acgattcgca atgttgagtc cgcatctgca cggaccgctc gcggacctct acgcacagct
    12841 cgagagggcg gaggcgcgcc atttcgaggt gtatctcgag ctggcgcagg ccgtggcacc
    12901 ggatcagtgg ggcgcgcgcc tcgccgagct cgccgagtgg gagagcgagc ttgcgaccgc
    12961 gcccgacacg ctgttgcgtt ttcactccgg gccgatcgga gcggatctca cggagcgatg
    13021 cgatcgtaca cgtgcttgat ctcgagctgc ttcaggtgcc cggccttgat gtcgctcgtc
    13081 acgatcagcc ggccgtcctc gtccagcgcg tagtcgtcct cccgccagcc gctgttggtt
    13141 ttggtcgtgg tgacgaacac cggtccgcgc caacccgact cgcgctcggc ggtggagtcc
    13201 tttccgtagg gcaccttcgc cttggtgccg gcgacgaact catcggcgct gcccgtgcca
    13261 tcggccattc tcgagcggat gctcaccttg ggcccgtcct gggtgatcac gagcaggctc
    13321 ggagccgcca gcactcgagc cagcactgag cggccgcggt tcgccgaagt gtccgcctcg
    13381 ctcggcgtgt tgctcggcgg cagaggattc gactgttcga ttccgggggc aggcgccggg
    13441 ctcgggccac cggtccactg ctcactcagg gaggactcgc gcgcggtcgg accagagatc
    13501 ccgtcagcct caaaggagcg tgcatccagg ggcgcggagc tgcctgcgaa gcggggaact
    13561 ggaccgtcag gcgtgcccat cggtggcagg cccgtgtcgc ttccgggatt ctggtgtccg
    13621 tgatggcgtc cgctcgatgc cggcttgctc aggctcttgt cctggtccag cagccgatcc
    13681 ggatcatcac tcaggttgag atcgagcttc cagttgcccg tcaggttgac cccgccgggg
    13741 gcactcgcga ccaggcgatc actggcacag cctgcgaggc aggccgccca gagcgcaccc
    13801 caggccagca aaatgagtgc gcccgagcgc ttgcgtgtgg acatgctgtt cctcctggca
    13861 ggcgggccaa ctcccgcacg ccaagtgtgc cggcttcgac cgaacattgc tcggggagtt
    13921 ctgcggcgcg agattgctcg gggagtcctg cggcgcgagg tagaatccca gccggacagg
    13981 gaggtccaaa tctcctctct gcacagttcg ttacaacatc gaggcccgat gagcgatcga
    14041 ccccaccttg tcatcgtcga tgacgaccgc gagatccgca ccctgctcag ccagtacctg
    14101 gagaagcatg acttccgcac gaccgccgtg gccgaaggca aggaaatgcg ccgcgtgatg
    14161 gagcgttcgc gggtggatct cgtggtgctc gatctgatgt tgccgggcga ggatggactg
    14221 gccatctgcc gcgagctgcg cacccgctct caggtcccga tcatcatgct gacggcgcga
    14281 ggcgaggatg tcgatcgcat cgtcggtctg gagttgggcg cggatgacta cgtccccaag
    14341 ccgttcaatc cccgcgagct cctcgggcgc atccgtgcgg tgctcaggcg tgcggcccat
    14401 gcgccgcgcg atccgagccc cgacacggtt cgctccttca ctttcaacgg ctggaagctg
    14461 gacacgacaa cccgcacgtt gacggacgcg gagggcaaag aggtcgcact gagcggcgcc
    14521 gagtaccggt tgctcgcggt tctgctgtca tcggcgaatc gtgtgctcag ccgcgcgcag
    14581 ctcactgagt tgctgcgagg acgcgaagcc gaccccttcg atcgaagtat cgacgtgcgc
    14641 gtcagccgct tacgccagat tctgggcgat gacgcgcggg ctccccagat catcaagacc
    14701 gtgtatggcg aaggatacgt cgtcggcgtt gccgtcgaga cgcactgagc cgggcgcgat
    14761 ggagagttga ggatgcgcct gtcgctgtgg ccccagtcgc tttttgggcg gctgctggcc
    14821 gcctccgtgc tggcattgct gttggccgag gctgcgagcc tggtgctggt tgcgcgtgag
    14881 cgtgagcgct tcgtgctgca aggcaacgtg cgtgaatggt cccggcgcat cgcggaagtg
    14941 accacgatgc tgcaggggct gcagggggcc gagcgcgatg cggcggctca aaggctcacc
    15001 gcgggaccgc tgcatttcgg gcgccgggcg gacggcgtgc gcctgcgcag tgaagctgat
    15061 gaggatggcg agccgcctcg tcgcggggcc cgagatactc ggggctcccc ttcgcccggg
    15121 ggccgtttcg accgcagcac cgcgagtctg ccattcgtgc ccttcgccac ccagagcgaa
    15181 ttcgagcaca catttgccga acagctgcag acgaccctgg gccgggactt ccgcctttct
    15241 gtgagcccca cgacggatcc gtcgcggcgc gccatttcca tcacggggac cgcctttgcc
    15301 gagaatcgtg acggacccgt cgagttgtat gatgcgacag ttggatttcc ggacggctac
    15361 acggccgtgt tccgcgtggg gcgcgtctcg cggggtccgc cgctgcctcg gggcctgttc
    15421 ctcaacctga tcctgctgat cgtggtcatg ctggtggccc tgttcgtcac ggcccgcagc
    15481 atcacgcacc cgctctcgga gctggcccgg gccgccgatc gggtcggtcg ggacctgcga
    15541 cagccgaggc tcgccgaacg gggcgcacgc gagctgcgcc acgccgctcg cgctttcaac
    15601 acgatgcagg atcggttgca gcgctatctg gacagccgca gccgcgtgct cgccgccatg
    15661 tcgcacgacc tcaagacacc gctcacccgg ctcaggctgc aggtcgagac gctcgaggat
    15721 ccggtgatcc aggcgcgctt cggcaagcag ctcgatgaga tggagagcat ggtgcacggc
    15781 gccctcgctc tgtttcgtgg cctcgatgac aatgaggcgt tcgcgccgat cgacatggat
    15841 gcgctcctgc gcacccttca atcggagttt gccgagatga atgcgtcggt catcttgcag
    15901 gggcgagctt tgcgcccgat ttccgccaaa ccgcaggcgc tcaggcgttg cctgacgaat
    15961 ctcatcgcca atgccgtgaa gttcggttcg cgcgcgacga tcgtcatcga agacggccag
    16021 gctctcatcc tgcgcatccg cgatgaaggg ccgggcatcc ccgaggagga gctggagcgg
    16081 gtcttcgagc ccttctatcg attggagact tcccgcaatc gcgacacggg gggcacagga
    16141 ctgggcttga gcatcgcgcg agatgtggcg caggcacatg gcggctcgct cgtcctgcac
    16201 aacctgccgg tgcgcggatt ggaggcagtg ctcaccctgc ccagaatctc ctgagctcgc
    16261 actctggcgt cgcgcagagc tcagcgaggc agctcgcgga gcgcgtactg acctctctcg
    16321 tggatgaggt agcttccctg ctcgtaccag gcgcccaaca cgaggcgctg cgcaggcttg
    16381 ccatcgacgc tcgtgtcgtg cacgccgggt cgatgggtat gcccgtggat gatccggcgc
    16441 acgcccgtcg cgcgaaacgc agtggcgacg gcatcgggat tgacatccat gatgtagggc
    16501 accgtgcgca agatatgctc gcggctgccg gcgcgcgcct catttgctag cagctcgcgg
    16561 tctgccagcg gcaacgacag gaagcggcgt tgccactcgg cgctgcgcac gatactgcgc
    16621 agttcctgat acgaatgatc gtccgtgcag agcgtatcgc catgagtgag caggacgcgt
    16681 tcgccctcga ggtcggcaat ggtgggatcg gtgagcagcc gggcgccggt gcggcggcag
    16741 aaatcgtgac cgaggaggaa atcccggttg ccgtgaatca ggaagcagcc gacgccgctg
    16801 gcggtcaggg cgcgcagtcc ggcgcgcacg cgctccttgg ccgggtccgc atcgtcgtcg
    16861 cccacccagg tctcgaacag atcccccagg atgtacagcg cctgcgcagc agctgcctgc
    16921 gtgcgcagga agctcaggaa ctgctcaacc gcatccgggg tgcccgcatc cagatgcaca
    16981 tcggacacga acaagcggct catggcctcg cgccaggccc gtcacttggg ggagagaatg
    17041 gtatcgggcg aggcgggcgg gaccgcggtc ggagcaggcg ctgcgacggg cccgttcgtt
    17101 ggggagccgg catcgatcag ctcgattttt tcgatcacga ccggcttgag cggcgcatcg
    17161 gacttgaacg gtcccatggc gcccgtcggc gtcaccccga ttcgatcgac cacgtccatc
    17221 ccctggatga ccttgccgaa gacggtgtag ccccagcgcg tgggcagcgg atccaggtcc
    17281 gggttgtcca cgagattcac atagatctgc gcattgcccg aatgcgggct ctcgccccgc
    17341 gccagcccga ccatgccgcg cttgttctgc aggccgttgc cggactcatt gaccaccgca
    17401 tcgtgcacgg gcttgagctt gtagtccgtg gccgaatgcc cgccgccctg gatcacgaag
    17461 ttggcgatca cccggtgaaa cagcgtcccg gaatagaacc cctcgtgcac gtagcgcagg
    17521 aaattcgcga ccgtgagagg ggcgcggtcc ggcatcagct cgatgacgaa ttcccccatg
    17581 ctggtggtca ctttgacctg cggtggcgac tggggggcgg cggcaggcgc ggcgggtggc
    17641 tgcggctgcg caggcggatc ggcggcccgg acaggcccct gcagcaggag tccggcgaat
    17701 aacagcagag cggttgcagc cggggtacac ggcctatacc agggaagaat gcgcatcgag
    17761 cctccatgag cttcggcggg aatactagta cgaatggcca catccatgtg cctgagcagc
    17821 tggaaatcgc gcctgagcgc tgtgtcccgt aacatgccgc catgagcacg atggacactc
    17881 cggcgggcga gtcgttcggt gccggcggcc cggtgacgcg cttcgcaccg agcccgaccg
    17941 gcgcgttgca cctgggcaat gcgcgcaccg cgctgttcaa ttacctgctg gcgcggcgcg
    18001 ggggcgggcg attcgtgctg cgcatcgagg acacggacgt gcagcgcagt cacgaggatc
    18061 atacgcaggg gctcatccag gacctgcatt ggctcgggct cgagtgggac gcgggaccgg
    18121 atcgcgagga ctctctcggt ccctatcggc agtccgagcg tacggcactc tacgagcgct
    18181 acttcgccga gctggaagcc gccggtgcgg tctatccgtg cttctgctct gccctcgagc
    18241 tcgaagcctc gcgccgtgcg cagctcgccg ccgggcggcc accgcgttat gcgggcacgt
    18301 gccgcgagct cacgccggcc gagcaggcca gcaggcgagc cgcgggcgcg ctcgccagtc
    18361 tgcgctttcg cgttccgctc ggacgccgga tcgagttcac cgatttcgtc catgggccgc
    18421 agtcctttct ctccgatgac atcggagact tcgtcgtgcg ccgcacggcg ggtaccgcgg
    18481 ccttcttctt ctgcaacgcg gtggacgatg cgcgcatggg cgtcacgcaa gtgctgcgag
    18541 gtgaagatca cctgacgaat acgccccgcc agctgatgat tctcgaggcg ctcagcctgt
    18601 ctgcaccggt ctacggacac atctcgctgc tggtgggaaa tgatggcgcg ccgctctcga
    18661 agcgtcatgg ggcgaccagc gtacgccagt accgcgatcg cggttatctg tccgaagcga
    18721 tgaccaacca tctgttccgg ctcggacatt ccactgccga gcacggcttt ctcactctcg
    18781 agcagatggt gcgcgcattc gatgtcaacc atctcgggcg ggccccgtca catttcgatg
    18841 agcagcagct caatgtctgg cagaaggagg gtgcccatcg cctgagcaga gatgcagcct
    18901 tgcgctggct cgagccggtg ctgccgcagg gaatcgatga gcggctgcgc cacgaattcg
    18961 tcgccgcgat tctgcccaac ctggtgttac ccgaagatgc gcgcgtgtgg gtcgaggtgg
    19021 ttttcggcaa gcctcccgac ctcgaggcaa aggatgagca ggtggtgcgc gaggccggcc
    19081 ggggctactt ttccgcggcg gcagccgccg cggcggccag tggcaacgat ctgccggcca
    19141 tcgcgggtgc ggtgcgtgcc gccacaggca ggaagggggc ggcgctgtac atgccgttgc
    19201 gggttgcgct gacggggcgt gcgcatgggc ctgagctcgc accgctggtg agagccatgc
    19261 cggccggcca ggcgcgcgag cgcctggccc ggttcgcctg acgaggcggc ttttccaaca
    19321 atcgaaacgg tcatggtccc cattcgcatt cacgattcga tgacgggtga gaagcagctg
    19381 ctgcagccga tcacgcccgg gcacgtgcgg atgtatgtct gtggcatgac cgtgtacgac
    19441 tatctgcaca tcggtcatgc gcgaatgctc acggtgttcg acctcgtgag ccgctatctg
    19501 cgtcatcgtg gctatcgggt cactttcgtt cgcaacatca ccgacatcga tgacaagatc
    19561 atcaagcgcg ccgccgaggg caatgagccg atcgaggcgg tgaccgagcg cttcatccgg
    19621 gccatgcacg aggactgcca ggcgctcggg ctcaccagtc ccgagttcga gccccgggcc
    19681 acgcagtacc tgcccggtat catcgacatg atctcgcgac tctgcgagcg cgggtatgcc
    19741 tactcggcgc ccgatggcga tgtgctgtac gccgtggcga agttcgaagg ctacggccgg
    19801 ctctcgggca agaggctcgc ggatctgcgc gcgggcgccc gggtggaggt ggacgaaggc
    19861 aagcgcgatc cgctggattt cgtgctctgg aagcatgcca agcccggcga gcccttctgg
    19921 gagtcgccct ggggccgcgg ccgccccggc tggcacatcg aatgctccgc catgtccacc
    19981 gcactgctgg gcacgcactt cgatctgcac ggcggcggca tggacctgaa gtttccgcat
    20041 cacgaaaacg agatcgccca gtcgtgcgcg gccaccggcg accggtttgc caacctgtgg
    20101 atgcacaacg ggttcgtcaa catcgacaac gagaagatgt cgaagtctct gggcaacttc
    20161 tttaccgtgc gcgagatcct gccgcggctg cgccatccgg aagtgctgcg ctacttcctg
    20221 ctctcgagcc actaccgcgg cccgatcaac tattccctcg atcagctcga tcaggccgac
    20281 ctcgctctgg gccgcatcta caccgccctg cgagaggtgc ccccagggcc tgtcctcgcg
    20341 agtgctcaca ctgctggatt tcatgcggtg atggacgatg acctcaacac gccggaggcc
    20401 ctcgccgcgc tgcagaacct gactcgcgag ctgaactcgg ccaaggccgc cggcgagagc
    20461 gaccgggcag cggctctggc ggccgagctg cgctcgctcg caggcgtgct gggcctggcg
    20521 caacttcagc cgcaggactg gttccggcgt gcgagtcccg ctgcgcaggg gagcgttggc
    20581 ggcgccgggt ccggggctcg ggccgaagcc ttcagcgatt cggacatcga aagccgcatc
    20641 gaggcgcgca atgccgcgcg cgcggcccgg aattgggccg agtcggatcg gatccgggac
    20701 gagctctcgc gtgcggggat cgttctggag gacaagcctg gtgggcggac cacctggcgc
    20761 cgcgcctgag gagcggcgtt acccaatcag cctcgccagc caccacgtcc ccgcggcgat
    20821 cagtgcagcc gccgggagcg taatgatcca ggctaggacg atatccccgg caacacccca
    20881 gcggacggcc gaaacacgct gcgcggcgcc taccccgacg atcgcacccg tgatcgtatg
    20941 agtcgtggag accggtatcc ccaacgcgga agcgaggaac agtgtcagcg cccctcccat
    21001 ctcggcggag aagcctccta cgggcctgag cttggtgatg cgctgaccca tggttctgac
    21061 gatacgccac ccgccgaaga gcgtgccgag cgccatggcg gcataacaac tcgcgacgac
    21121 ccagaagggg aggtgatcgc gcgaggtcat tccggctgaa atcaacatga gccagatgag
    21181 gcccatggtc ttttgcgcat cgttgctgcc atggccgatg ctgtacaggg ccgaggacag
    21241 cagctgaagg cgacgaaacc accgatcgac catgtacggg gatcggtgga agcagatcca
    21301 cgagatggcc accagaaatg ccgctccgag cagcaccccg agcagcggtg agatgaccat
    21361 gaaggcgacc gtcttgagga tgcccgtcat gatgaggggc gccgtccccg cgcgcgccat
    21421 gcccgcgccg atcatgccgc cgatgagcgc gtgcgaggag ctcgtcggca gaccgtagta
    21481 ccaggtcgca agatcccaga ggatcgcccc tgcgagagcg ctcgcgatga cgcgcgcatc
    21541 cacgaactcc ggcgcgagaa tgcctttgcc gatggtggct gcgacgctca gcttgaagat
    21601 cgcaaaggcg acgaagttga agaacgccgc ccaggcgacc gcctggtaag gccgcaacac
    21661 gcgggtggag accaccgtgg cgatggagtt ggcggcgtcg tggaagccgt tcagaaaatc
    21721 gaacgccacg gcgagcgcga ccagcatggc cagggctgta aagctcagcg tgatatgcat
    21781 gccggagagt cgagtgcgct cgggcggtca ggcattctcg agaacgatgc cctgaatgat
    21841 gttggcgacg tcctcgcacc gatcggtcgc tgactcgagc agctggtaca cggtctcgag
    21901 cttgatgagc tggcggacgt cgccttcctc ccggaacagc ttcgagagcg ccgcccgcat
    21961 cgtccggtcg gcctgggact cgagcccgtc gatctcagta cacagctcca gggtgcgcgc
    22021 cgactccccc gtgctcgaca gcagatgaac ggctttctgc aggcgcaggc agcattcgtg
    22081 caccagatcg ccgagctcgc aggcctgggg cgtcagctga cggacgtcgt agagttgaaa
    22141 ggattcggcg gcgtcctgga tcacatcgag gacatcatcc aggcgcgaga ccagcagatg
    22201 gatgtcatcc cggtcgagtg gcgtgacgaa ggtccggtgc agcagcccga atgtctcgtg
    22261 agcgatcttg tcggctcgat gctcgagctc gaggatctct gcgattcgcg catcgcgctt
    22321 gtccgcttcg tgcgtgaacc ctctcagcag ctcggccagc acggcgccgg cagcgaccac
    22381 cagcgcggcg tgctggtcga agagctcgaa aaacctgcgc tcgcgcggca tcaacctgga
    22441 aaggaactgc accgtcgatc tcctcgttgg ctccctcgag cggcggacat cggcctgacc
    22501 gtagctgtag gaaggaatgt tacgccagcc acccgctgtt gacaggtcaa tccgagccgc
    22561 agcacggcag atcgaggggc ccgctcgcgg cccaggtgta ggtacggcaa ttgctgaatc
    22621 tgtagctctc accctaccgc cggccccgcc ggaaggagcc cgactatgca cctcaggttg
    22681 cgtgcaccga ttgttctcgc cgccttggcg atccttgctg caggaggctg cgccgctccc
    22741 gagcatcgca tgaacgtcgc cgcctcgcgg gatgataccc gtcgtgccga cttcgagcgt
    22801 tgccggcagg aagggcgtac cgactgcgat gccattctca atgcaccggt gaattccaac
    22861 acgccaccgc gtggtgactc ggtgcgcgag caggagcgac gtgaggccta tgaccgctgt
    22921 gtggcgcgag gcgggtcgga ctgcgaagat ctgctgcatc acgattgaca ccaggggcca
    22981 gcccccctga gcgaggccgg gacgcggggc gagcactcct gtggcccgcg gccctctggc
    23041 attgcgcttc gaaggcaacc tgctacgatg cccgccgcgc cacagccgtg gcggcggtcg
    23101 gggcgtagct cagcctggta gagcatctgc tttgggagca gagggtcgca ggttcaaatc
    23161 ctgtcgcccc gaccaacact cactcggtct ctctaccggt cgcatcggtg acttggcctc
    23221 agtgaatcgg cgcaacactt gcgtcgagaa tgtatacgtt tggtgctttt cactgctgcc
    23281 tctctgcggt cttgtgtccg gcacggaatc cgcggtgcga tacctgccgt gacttggagc
    23341 acttgggaca ccgccgcgtc cctcccgctc cagtcaaaca ccggcgctcc gcggccgcct
    23401 tactgcctcg gtttcccatc cttctgcaac ccagatctcg gtccgccgcc cgtgagtgtg
    23461 ttgtgcgccg cttccatctt ccacgcgcct gcatctttag tgcttgcctt cgggcatcgc
    23521 acgagtgtgc tcagaagcct gagcgccggt cgttcttgcg ccgcgagttg attctgcgtc
    23581 aggctgtgtg ttcgtgttag cgggcgggga tcggcgcacc tagggccaac cagtggcggg
    23641 tggattgaag ctgttcgagc gcgcatctgg cacctgagga gatgaagtta tcgccgcagt
    23701 tcgcaaagca aacgtgcaga tgacaagaat atcaacggta cccgtgacgt aaaggcggtc
    23761 tgcgaggcgc aggcacgcta gccgaccttc agcgttcccc tgaccggttg ctttctggca
    23821 ggtggcagcc cacaaggcgt gaaacccgcc gcgtgcgcag gcatttgtcg tgtcgcgtca
    23881 gataccaggt atttttggct tggcgcctgg aatgctgccg gggccccgga ccctggcgag
    23941 gaaagcgcta gcctgtcaaa ggtcagtgcc ccggtcccag cgatgggcgg gcgcgcgacg
    24001 cccgggcgac aatggtcccg agtcaagtgc ctcatccggc ttgcgaggat aagttggtgt
    24061 cctggggcct gcttttgggc caagatccag ccttaagagt ccttaacggg aactcgatca
    24121 gactgaggtc gttgcgatgg ggcgaacaaa atgaaatccg gtggctgggt gagccgtact
    24181 gataccgcac gcctccgtga gctggagtcc ctgtacgcgg cgctcaataa ctcgcaggcc
    24241 gtgatcgagc tggatatgaa gggcacgatt ctggccgcga acgataatta cttaaaacta
    24301 ttcggttata cgcgtgcgga atccctcggg cagccctact cgctgttcgt caatccagcc
    24361 gaccgcgagg ggcccgagta caaggccctc tgggacagcc tgcgctccgg ccggtatcag
    24421 caggggcggt tcaggcgcat gggcaagaac ggtcgtgagg tctgggtccg cgccagcttc
    24481 aatccgctca tcgatcggga tggcaggccc tggaaggtga tcaagctcgc ggcagaaatc
    24541 actcacatca tcgtcgaagg gcgcgcgctc gactacgccg tacaggagtc gcaggaagtg
    24601 attcagtccg ccctcaaggg cacgggggat cggcgaatca gtctgaacgg caagaccggc
    24661 catctcgagc tgctggccgc aggtgtcaac gagttgatcg acggcgttca aaagacagtt
    24721 gcggaaacga tggaggttgt gcagcgcgca gtcgacggtg atctgacttc acgcgtgaga
    24781 gtcgatgata agtcgggaca cttcagaact ctggcggtgt ccgtcaattc catgatcgag
    24841 ttcatgatgg acgtcgtcac tacgttgacc gaggcgtcgc atgaggtgca ggtgagcgcc
    24901 gaggaaattt cccgcggtaa cgccaacctg ggcaggcgta ccgcggagca ggcctccagc
    24961 ctggaggaga ccgcttcttc gatggaagag atgaccacaa cggtcaagaa gaacgcggag
    25021 catgcggtac aggccagtca attggcggaa gctgcgcgcg agcaggccga gaggggcaac
    25081 cgtatagtgg gctcggcggt tgcggcgatg ggagagatca acaccgcgag taagaagatt
    25141 gccgacatca ttggagtgat cgatgaaatc gcctttcaga ccaacctatt ggcgctgaac
    25201 gccgcggtgg aagcggcccg cgcgggcgaa cagggtcgcg gttttgccgt cgtggctacg
    25261 gaagtacgga ctctcgcctc gcggagtgcg gctgcggcca aggagatcaa gcgattgatc
    25321 caggactcgg tcggcaaagt ggacgaaggg acgaagctgg cagatgaatc cggggcggcc
    25381 ctcagcgata tcgtgaggcg cgtcaagagt gtgaccgccg tgatgacgga gatcgccggc
    25441 gccagtcgtg agcaggcatc gggtatcgag caggtgaata gagctgtcac cctgatggac
    25501 aacatgacgc agcagaacgc ggcgatggtc gaagaggctt ccgcagccgt gcagaggctg
    25561 atcgagcaag caactgccct gacaagattg attgcccggt attgcaaatc cgaatcgcgc
    25621 ccgagcgccc tgataccaaa tgtgaggtaa accgggagga cgtcgctgca cgctcagcga
    25681 gacgacgggg gcggtgcgca gctttctcat ctgtacacca gcaatgcgtt ggtgctggaa
    25741 cgtgtcgagt acccgacagg gactcgctag gagggagagc gcgccggggg cgcggacgca
    25801 ggtcacacgc cagcggcacc atcgcgcagt acgacggcac gcatctcctg attcgctatc
    25861 cctcactcat gtatggggag ctgtcggtct atatataatc cggccgcgat tcagttccga
    25921 ggccttgcgc ccgtagctca gctggataga gcaccggcct tctaagccgg tggtcggggg
    25981 ttcgagtccc tccgggcgcg cactggcggc aagtcgtata tactttcgcg ccccgtggga
    26041 ggggcccggt tgcggggcaa cgtggcaatg ccacgctgcc gtcaaggctg cttcgggcgg
    26101 aggcgccggg cagacataag tccttgattc gattcgggcc ttgacgatgg tggacgtagc
    26161 tcagttggta gagccccggg ttgtgatccc ggccgtcgtg ggttcgagtc ccatcgtcca
    26221 ccccatccat ggcgctcagc gggtaaacgt cacaccgttt ccccgcggca tgcgaagtga
    26281 tgtctgctca aagcgccgcg ggcgactttg agccaaatcg acgagggccg ttagctcagt
    26341 tggtagagca ggagactctt aatctcttgg tcgtaggttc gagtcctaca cggcccacca
    26401 cttttccctc taatagtcac gacccaccct ctaagtttta gcgcacaggt ttggtccgga
    26461 gcatcttccg atacaccgct tccatcgccg ccccgtggcc cagcactgag tgcggggagt
    26521 ccgtctgcgc tttcgcccgt aggtcccgga aagcccacgt caccttgagc cgccgcatct
    26581 ggctgttgat ggcccacaca ctccatggct gcccgaagcg attggcgaac acatgatcgg
    26641 tgtccgggaa tctcgccatg gcgcggcgca caaagaaccg cagcgcatcg gaccactcca
    26701 cggtgtgcgg tttccccgtc ttgctctcca cgaacacgat gccctcgggc ttcaagttgc
    26761 cggagcgcgt ccaggagacg acatcgcctt ggcggcagcc cgagaggtag gctgcggcga
    26821 tcaagtcctg gaacggctca ggggaggcgt ggaaagcctc taagaattcg gcgtctgtga
    26881 cgttgcgctt acggggtttc tcggtatttc gggcgatccc gcggcaaggg ttcgcctcga
    26941 gccagccctg gcgcatgccg aaattgtgca cggaggccag gacggccatt tcccggttcg
    27001 cagcaatcgc cccccggccg cgtttgcggc gcttctctaa gtgcatggcc acgtgggacg
    27061 gtcgcagggt gccgatccgc atcttcccga atgtcttgtc cagtcgggtg agcatgaggc
    27121 catagcgcgc ctgcgtagag gggcgcaggt tgtccatgcc ggcagcgcgg tacacggcga
    27181 tcatctctcc caccgttccc ggccttgcgg ggtcgagctc gtagagcgct cgatagaggc
    27241 tattgatccc ttcgtcgatg cgtgagagcg cgatccagcg attgcggaca accttgtaat
    27301 agcggccgtc tttctcgaag acgccgggaa ttgagagtcc gcaagcgagc tttcccatga
    27361 atagtcgggc tcgttctcgt tatcccggcc acgatacagt gcgtggttca gcgcttccac
    27421 tgtggtgcag gggcggccct tggcatcccg gaacgtcagt actcgatttc gcgcacacca
    27481 tgccgccacc gcacccacct tgcgggtttt cacgaaagag gccagggcgt cgaaatcgac
    27541 ggtgctcatg cgcgtttagg acgcccggca gagcgtggct ggcaagccgc atcgatctta
    27601 tccagcagtt ccatgatgtt cgtcacacgt tccactacgt gcttcttatt ggccttgctc
    27661 gcatgggtca ctacgaactc gtgaaccttt tcaagtattt gctctacgtc gatcagtgga
    27721 gataacgcgt gatcgaaaaa gcgctgttgc ggcgtatcgc gctttgcagc ctcctgtttc
    27781 tctcgttgct ttgcgcgctc gtccgcttga cgctgttttg cctgagcctc ctgccaggac
    27841 ttttcattag cttcgcgtac gcttttcgcg agcggcgatt cgtcagctat tcggcccacg
    27901 agaaattcgg cgcggcccga aacactgacc tcatcggcta ggcgatcaat ctcagattta
    27961 tcttcgaacg aagccagttc ttcgccgcgc ttagccttgc tgctccatat gcgccgctct
    28021 gccaatacct tgtacggctc cccaagcatc ttcgcgcaaa taaacatctg tcgaagagcg
    28081 tgggctttga gcttctgtgc ctcaacaatt gcgcgattgt cttcgtgaag acgtccccag
    28141 agcgcgagtc cctcggctgc caaaagaacg cgtttcgttg tgtcgatatc acggacggag
    28201 gccaattgct tcaccgcctc ttcataaact atcggcggcg acggagcatg acgtaattgc
    28261 tgagctacta tgtcgcgctc agttggcggc agtttcgaga tttcgtgccg gatcactgcg
    28321 ggaagagtta gcgacatgag ttctccaaat gaattgctgt tgagaatgat gcaggaattg
    28381 cacctgctga cgcctttccg gtggggatat gctgcaggca tccaatccac tccagggcat
    28441 cgcggcctag gaaaccgctc gtgtcactgt ccacgacgat cattcatttg ctctgttttg
    28501 cacaggtgct cacgccgcct tctccgctgg ctccggctcg aggcagactg gcgccagaag
    28561 tctgcatgcc tggaccgtgc gccagttcga cagcagcacg tcgcggatct catcgggcag
    28621 atcgccgcgt atgcacacga gcgtgatcag gcccaccagt tctgcgagtg cgtcccgatg
    28681 gcgccgcagg ctggtcatcc gcccttccgg cagctgctcg cggtgaagta gggctatctc
    28741 gcgcaggaaa tcgcggcttg ccacatcgcc gtcgatgaag tagccggcgg cgtactcctt
    28801 gacgtcgagc acctcgccgc gcatctcggt gtacgcctcg ctcagatagg caagcccgca
    28861 ctcggtgcgc acgcgcgcgc tcataacttc cccttgcaaa tccgacgcat caacttgcgc
    28921 ccctttctcc gtcgattgga tgcgatgatc caagcggccg ccctcgccac cacgagggga
    28981 aatcgtgtca gggaggacgt gacaggggcg gccatagcgt tactctgccg atcaggcaga
    29041 cggtggtgcg acagccggag tcggcggtgg cggtggcggc gtattcgcgc tgaccgcagc
    29101 ggccagggcg gcagtgctgg tcgtgatctg cgtttgtagg ccgttgagcg cggcgagctg
    29161 atcgggcgtg gcgccggcgg cagtcgccgc agccaccgcc gcttgcaact gcgcagcgaa
    29221 gccgttgatc agcgtaatcg cactctggtt gacggtctgc tcagccgtga cggcggcagt
    29281 cagttgggtg atttcattag aaagagaatc gacggtagcg ttcacgtttt ggagctcctg
    29341 taggatcaac gacaacatct gatgtaactt ccggttgaaa aacatcacgc ggcatccacg
    29401 atgggtggcg catcgctatg aacaaactgc tcagcctgtc ggttcagcaa tgcgagtgcg
    29461 ttcgtgaagc ggaaattgtt ctggatctgc gcgcggatct cgccgggcag gtcctcgcgc
    29521 gcgctgataa gttgcaccag gcccacgagg tccgagagca cggcccggtg gcgtaccaca
    29581 accggcatcg cgcctgagtg ctgccgacga tgctcgtggg ctatgccacg cagaatgtcg
    29641 cgcgcggctt cctcggattc gagaaaccat tgcgcgcaca actcgcgcat atccagccac
    29701 tcggcccgct gctcgcgggc ggcctcggag aagttgacaa gactcatgcg ccctccgcaa
    29761 gccgctccgg cggcagccaa gtgagcctcg ggagcacccc gtagagttcc gctac
//
LOCUS       DASRHN010000420        21092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1316163, whole genome shotgun sequence.
ACCESSION   DASRHN010000420 DASRHN010000000
VERSION     DASRHN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21092
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316163"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFB37_16990"
     CDS             complement(<1..512)
                     /locus_tag="VFB37_16990"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZO24190.1"
                     /translation="MARLNTRDYKKSHRRSVQLGRAREFAVGAFFGVVLGATAVYYVS
                     SRHQAQHDKTEAPRPDPHRQAPADPEAAATPGSGEKYDFYEMLPNFEVVVPEKDKEVK
                     RDLPTAAPVERPGVYVLQAGSYRNESDADRVRAQLAMQGIDAKVQRVAVDADVWHRVR
                     IGPITRLDEL"
     gene            complement(600..2357)
                     /gene="argS"
                     /locus_tag="VFB37_16995"
     CDS             complement(600..2357)
                     /gene="argS"
                     /locus_tag="VFB37_16995"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZO24191.1"
                     /translation="MKQQIEQLLQTAVNGLAGSVLPQGIEPAAISVERTRESQHGDFA
                     TNVALRLAKSAKRSPRELAQAIVAALPANALVSRAEVAGAGFINFFLSPQAYSQELAR
                     IHEGGDRYGESTLGAAQPVMVEFVSANPTGPLHVGHGRQAAYGATLSNILDAVGFRVS
                     REYYINDAGRQVDIFAVSVWLRYLELKGASLPFPANGYRGEYIRAIARYVCLQVGERF
                     QRPATAVLEHLPADEPAGDKEAYIDALIERARQLLGSDDFHRLVQLSLEQMLADIRTD
                     LEQFGVRFDRWYSERALSESGAIDRALERLRQQDRLYAKDGATWFRATEFGDEKDRVV
                     VRENGVRTYFASDIAYHLEKRERGFERLIDVLGADHHGYVARVRAGLVAMGEPGDSLE
                     VNLVQFVSLYRGGEKVAMGKREGQFVTLRELRAEVGNDACRFFYLMRSHEQPLDFDLE
                     LAKSRTNDNPVYYIQYAHARVASVMKQLASRGFAFDLAEGIASIDKLSKEHEQAVLGA
                     VTRYPEVLELAATSRAPHALVHYLRELANAFHTYYNAEQFIVADSKLRNGRLALVQAV
                     QQVIRNGLTLLGVTAPETM"
     gene            complement(2622..4844)
                     /locus_tag="VFB37_17000"
     CDS             complement(2622..4844)
                     /locus_tag="VFB37_17000"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZO24192.1"
                     /translation="MSVERVLRVAFDVPLRRLFDYLPPRAAPGGAEVALSEVPPGVRV
                     RAPFGGQRLIGVVMEVTPTSDLPPERLKSVIEILDPVPVLDSTALELLRWAAEYYHHP
                     LGEVTAAALPKALRLGARATAVQESWCATGEGHEALARGEPRRSPRQRALLRYLVDHE
                     TVSAEVLTEQLPGWRSAARALVARNWAGSLEISPSTPQAPLGVRESGPPLAEQQRIAV
                     ESVGNSLGSFGAFVLHGVTGSGKTEVYLRLVERVLARGERSLVLVPEIGLTPQLVGRF
                     RERFYAPMSVLHSGLTDHERLLAWREAHSGRARIVLGTRSAVFAPVGELGLIIVDEEH
                     DASFKQHEGGFRYSARDLAVLRAQKANVPVLLGSATPALETLNNVNSGRYARLTLPRR
                     AEQAQAPRMALIDLRTSPVRAGISTQAIQAVQRHLDADGQVLVFLNRRGYAPTLLCTA
                     CGWVAPCSECDARLTVHLSAGRLRCHHCGADAPLPERCPQCGFATRSVGQGTERIEES
                     LAALFPQVARVRLDRDTVRRRGELEEAMRRMASGEARILIGTQMVTKGHDFPNVTLVV
                     VLNADQGLFSTDFRAPERLAQTIVQVAGRAGRAARPGEVLIQTEFPEHPMLQSLLTEG
                     YDGFARTALAERAQAAWPPFSRLAALRTSAQTASDALAFLTDARKLTQPPSGVQVLGP
                     VPAAMARRAGRHHAQLLIESPERSALHRFLEHWLPEVEALPSSRRVRWALDVDPLELF
                     "
     gene            4980..5546
                     /locus_tag="VFB37_17005"
     CDS             4980..5546
                     /locus_tag="VFB37_17005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZO24193.1"
                     /translation="MKLSDSISSSQSRALNQFLAGVELKAFKIAQAALRNEDDALDAV
                     QDAMLQLARAYADKPAEEWKPLFYRILENRIRDMQRRRTVRGRVISWLPLRSNDEDEE
                     PDPIGQAPSVEPQPPRRLEIAEAMGALERALQELPRRQQQVFMLRTLEGLDVAETAAA
                     MGCSEGSVKTHYFRALQSLRAQLGEFWT"
     gene            5543..5950
                     /locus_tag="VFB37_17010"
     CDS             5543..5950
                     /locus_tag="VFB37_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24194.1"
                     /translation="MSRIEEQMPGELERRLKGLLEESVAHVDGRIRSRLNQARHAAIV
                     EASRPRRPALLRPFVLMPTAGALAAAALVAFVLWPHSPQVEIPMTAEAGHSMEDLDLL
                     ADGDGLDLVSSEESDGSFFEWAADQTDTNETST"
     gene            5971..6252
                     /locus_tag="VFB37_17015"
     CDS             5971..6252
                     /locus_tag="VFB37_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24195.1"
                     /translation="MLLALAALTPACPTVLADNKAPPPPTDVELLEFLGSVDSGADAQ
                     GADDGSWIDYLSQTDIGKAARPADPNGPARAKQVASGTQPPSAGDKKDE"
     gene            6245..6787
                     /locus_tag="VFB37_17020"
     CDS             6245..6787
                     /locus_tag="VFB37_17020"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HZO24196.1"
                     /translation="MNKRQTLLLTILFAAALPAFGQAVPPPPSVQGPSSPPAAQSVPW
                     SSLSPQQQQLLEKFGVNWNALAPERQQALARGSQRWLGMTPEQRGLAQQRFQRWHSLP
                     PDQRRALRERWEKFQSLPPKEQSAVRQGFRRFQQLPPDRRRMLREQWRSASPAQRQHM
                     VQQAREHRMQRMQPHPPPRR"
     gene            complement(6790..7752)
                     /locus_tag="VFB37_17025"
     CDS             complement(6790..7752)
                     /locus_tag="VFB37_17025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO24197.1"
                     /translation="MKTPGNLSPLAVALTLLCPLLTPAAAAPPKPAAAPTKTAETARK
                     PPTTASVIAASTASDWRPLDERHTLYMELPSGRVIIELAPQFAPHHVANIEALAREGY
                     YDGLAIVRAQDNYVVQWADPEGKRSVKNAQRTLAAEFERPSRGVPFTPLPDPDTYAPE
                     VGFSDGFPAAVDPKFGRAWLVHCYGMVGAGRDNDVDSGGGTELYAVIGQAPRHLDRNV
                     TLVGRVVQGMELLSTLPRGTGALGFYEKSEQRLPIKSIRVAADVPPAERTELEVLRTD
                     TPTFAAYVEARRNRREEWFKVPAGRVDVCNVPVPSRPRSGTPAS"
     gene            complement(7776..9173)
                     /locus_tag="VFB37_17030"
     CDS             complement(7776..9173)
                     /locus_tag="VFB37_17030"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZO24198.1"
                     /translation="MPADAAPLGANTLIQASYFVTAALFILGLKRMSSPVTARSGIWW
                     AGAGMLVATLVTFTFPGMSNYELIIPAIVVGSLLAWWSGKRVAMTNMPQMIALYNGMG
                     GGAAAAIAAVELYRGESQGYAISILAVVGGIIGAVSFSGSAIAFGKLQGLITRSIRFK
                     GQQIFNLVLLLVALIAGALVALHLNAAPAVVSAFFALALLLGVTMTLPIGGADMPVVI
                     SLYNALTGLAVAFEGFVLNNAAMIIAGTVVGSAGTLLTQLMARAMNRSLGNVLFSGFG
                     DTGGAPTGEVTGSLKPIEASDVGVMMAFAQKVIVVPGYGMAVAQAQHKIWELCQLLID
                     RGVTVKFAIHPVAGRMPGHMNVLLAEAGVPYDLISDLDEINSEFETADVALIIGANDV
                     VNPVARNDKSSPIYGMPILNADKARNVIVIKRGKGAGFSGIENALFYLDNTRMLYGDA
                     QKAGAELIQAIKAVG"
     gene            complement(9173..9490)
                     /locus_tag="VFB37_17035"
     CDS             complement(9173..9490)
                     /locus_tag="VFB37_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZO24199.1"
                     /translation="MTGIVALYIFMLAAFTGYEVISRVPVILHTPLMSGSNFVHGIVL
                     VGAMVALGGAHTPLEKTIGIVGVLLAAGNAVGGYVVTERMLEMFKSSGARGAKPGSSK
                     GAH"
     gene            complement(9595..10719)
                     /locus_tag="VFB37_17040"
     CDS             complement(9595..10719)
                     /locus_tag="VFB37_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZO24200.1"
                     /translation="MPVTIGALRESTPQETRVSLVPEVADKLTQTGARILMERGAGVR
                     AQFPDASFKSVTWADSAAAVLEQSEVVLTVQPLSLQQIQQLKKGAVVVGFMQAYSRRE
                     EVKALKERGITSFAMELVPRISRAQSMDALSSQAAIAGYKAVLIAANHLQKFLPMLTT
                     AAGTIRPSMVLVIGAGVAGLQAIATARRLGAVVEAYDVRSATREQVKSLGAKFVDTGV
                     SAEGTGGYARELTEDEKKKQQEVLDSRIAAADAVVTTASVPGRPAPKIISRAAVERMR
                     PGSVIVDVAAEQGGNCELTRAGEVVDHQSVKVIGAVNLPATLAYNASEMYARNLLNFL
                     KPALNKGELTIDWNDEVFAQSCLTHSGEIKHEATRKAIEG"
     gene            10848..11522
                     /locus_tag="VFB37_17045"
     CDS             10848..11522
                     /locus_tag="VFB37_17045"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZO24201.1"
                     /translation="MLGIDLDRADFGFQDDLRIVASPAARGLSQLVLRTDVAGMPLEW
                     IDYKEAARLYHQEQVAYTCGSPLYKLYGGINAASGLRTVLEVNSIVATVGHTGNPGNT
                     RHDYIPPLNNQTLFRRDAYLCMYCSMRFASRELSRDHIRPFSQGGLDVWTNVVSACRR
                     CNNQKASRTPEQARMQLIAVPFTPTYAEYIFLKGRRVLADQMEYLLAHFPRSSPLHDR
                     IQRWLS"
     gene            11617..12531
                     /locus_tag="VFB37_17050"
     CDS             11617..12531
                     /locus_tag="VFB37_17050"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HZO24202.1"
                     /translation="MIFLIDASVYVFRAYHSMLPDMVDRDGNPVHAVFGFARFVGDLI
                     ERVRPQYIAVAFDQRLSNSYRSRIYPPYKANREPQPFGLAAQFECCREVCRHLGLAVF
                     THREYEADDVIGTLACLMREEGIRSAFITRDKDLAQLVRDGDLFWDYGAREQFGYHDI
                     QRYYGVAPERFADYLALTGDCVDNIPGVPGVGPKTAAALMKEFASLDELYDGLGRIAQ
                     MPLRGAQSLEQRLREHRESVYLARQLTRITCDMELGVGVEGLRRRPPDMPALSKLYDR
                     LGFGPFLRRQGERLALLPGPSAPSRSLL"
     gene            complement(12523..13155)
                     /locus_tag="VFB37_17055"
     CDS             complement(12523..13155)
                     /locus_tag="VFB37_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24203.1"
                     /translation="MDHIQFKANQTAASYVADGLDAPTLEAFELHMMGCAECLGDVEI
                     WRAIRRDMHRAQPSGRSTPRKSRQPVFSDWRMAASLVGAGVVGAAGGWVGKTTQVTDL
                     DSTHTAVFSMADTGRAADCTLLHLATDTQSAVLRIPNLPRDQQLLALDSNRRELSSSQ
                     YSARTQPDGSRLLRLDAQVLRDGSVHLEARRPDGSREPIGCLTSAALESE"
     gene            complement(13164..13814)
                     /locus_tag="VFB37_17060"
     CDS             complement(13164..13814)
                     /locus_tag="VFB37_17060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO24204.1"
                     /translation="MTYSVAPPAELCREKPADGAEQGDMSLLEALAGRGSLQNQLYVR
                     YRRPLLQVFLHRRIARDAAEDLLQRTFLQAIKKIRTEGLDDPGNLGGYLYRTACKLAI
                     AYWRGELSHRHDSDAELLSSLKDGALSLEERLDQEQLAKCVRELVSHLPVQRDREVLE
                     RFYLHEEPRTAIRESLQLTDMQFNQVLWRARQRFGDILRRHGLAAGESETAQSTER"
     gene            complement(13811..15010)
                     /locus_tag="VFB37_17065"
     CDS             complement(13811..15010)
                     /locus_tag="VFB37_17065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZO24205.1"
                     /translation="MRRPRATTTLCCLLSSVVSASAQEPAGFPCRPLTCSTLLPSRVD
                     DVGTNDCAYRVGNLWWVDYVAGSLAWRDRKPTQPVIVAVFDDGAYVDHEELRNQLWTN
                     EAEADGKAGVDDDGNGYVDDVHGWDFVDDSPDVSPKGECRSRFSHGTLMASLVAAERN
                     NGVGIAAPGSDAARVMILRVVGCHSGVDDTASPQRLIRALDYATRMGARILSFSAHWN
                     ATTPELDAAFADIADRPDSPRAAIVVASVPNKGEPSAGYPAAYRFRRIVRAIPIGNED
                     TISPGTSPAPAGLNLGSPSACVIGAGGPPDQYRLEQGSSNSTAILAGLLAGIWADPRY
                     AKLNTDEFLARVVQGRMLKTPRRSKPGARITYREGVPLADACLLEGDGRTARVCHATV
                     PQREAHP"
     gene            complement(15272..15859)
                     /locus_tag="VFB37_17070"
     CDS             complement(15272..15859)
                     /locus_tag="VFB37_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24206.1"
                     /translation="MSLTQKATLSASIAGQCLVAAFAAAGPASAAQIPEYFFREWTVT
                     KNCTEAHAGLAAQVAAGLRFRISRDSAAADGSYRFQAEDGAQQQWAANWNGLRLEYRP
                     GTQMTSVPADFECIPGQESSSPFLAMSGYAQASEPYYEQQHWYGLATIHGQLEHVLIF
                     PRNMKGASSAIIVLASVTAPSTIQLDDDGVIHSSD"
     gene            complement(15974..19207)
                     /locus_tag="VFB37_17075"
     CDS             complement(15974..19207)
                     /locus_tag="VFB37_17075"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZO24207.1"
                     /translation="MRIPAPAPGTLQVQIDERGLTLVSLLDERPSTEATSPVERFGTI
                     VLLTSTLAGQLHTVRIRPHDSPEMQGEFCLRARLLPRDDPALAEPLAAFAQGGQATQK
                     QNWQAAFDAYLKAARGFDAVGLRVDAGQARQALAELAYRRFDRKRDAYALTAEALADY
                     GERADPITKGLLTGLRASALLAMPGTEPRAEGARVRELLAGARRYDSASPAGARELPR
                     LDIMTGFLEYTLDAPARAQAAFTQAAEHCRKTRDWGCYAVATQNLALLQESSNYDSAL
                     QAYTQALRLLPVGLEPRLRADISNNLGRLQGVVGLFSASQRSHEAAMHEYAQLGDCPG
                     VRRSMSRLGTLMTQLGVVGDAESYLQRAASFDCADLLSAAGTPQSGLTQPAASDLPIP
                     QRNSAGAATPHRSLCVHPIDAEGLASENRIVVFNSLLSLGNVLMMEGELSQAQRCFQT
                     ARLYAADSRTQVRLENASGAMLLERADAAGARAAFNQALHIADVARLSSTYEHRGAAR
                     LGMVKAALLAGDAGDAMRQSYQTLRSSVLRGDVDQTVTSLRLLAAAYRGSRPQEAART
                     LLTAADLIEAVPIDQLDGEKRATYLATQYSVFADLTDLFASQAETDRDLAWRAFSTSE
                     RGRARSLRYAVTQATRAAADSAAPPPTARYREMLREAAQLTHADTAEPAEGLVQQLDN
                     LAQHERGATQPLDRESLLRTLDELSATLVEYAAGTQDMFAFVVHDGAVQVIRLAGRRE
                     IATASAELLERLRDPETPANTVRQAATKLARLVLWPVHAALSGKRIVFVPDDALHTVP
                     FTVLPWSASDSDQLVLQHAEVTVLPSARFLLRRPDRGSHRDDAPRVKLIGDPVFGLTR
                     WRRECLNGGAEASPAPPATRSAVDWLDSLPRLPGSRSEVLSIAKLMQEARPGARVDLA
                     LGCAAVPEALRRGAESAELLHIATHARVDAQRPRLSALALTPEAGDEASSAFGLLDIL
                     GLKLNSSLVVLSACDTSRGRLLPGEGVLGPAQAFLEAGAAAVIASYWRVDDQATAAFM
                     QQFYRHLLLDRLPASRALRQAQLERASSSSSYAWAAFALYGWPDSSI"
     gene            19308..>21092
                     /gene="acs"
                     /locus_tag="VFB37_17080"
     CDS             19308..>21092
                     /gene="acs"
                     /locus_tag="VFB37_17080"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZO24208.1"
                     /translation="MTAGTLQVIRAKRGSDVRGHVHMWMAKWNQSAASFYAIRSAPAK
                     TVCAAASVRVGAAILSIPGAIRLPYAGPRPWCRSLEGNLMASRNIESVLLEERSFPPP
                     SGFAARAGLKAADVERMQKQAAADPSRFWADLARQELSWHQPFSVSLDDSHAPNYRWF
                     TDGRLNVSFNCLDVHLAERGEKTALVFEGEGGDSRRLSYRELHAEVCRFANALKAQGV
                     RHGDRVIIYMPLVPEVIVAMHACNRIGAIHSVVFGGFSAPALKDRVVDTEAKLVITAD
                     GGWRGGSVVELKAAADKALASGCPSVQRVIVLRRTGKSVPMDPERDRWWHDVIEGQPS
                     TCEPDWVEAEHPLFLLYTSGSTGKPKGIQHSSGGYLLGAKLTSRWVFDLRDDDVFWCT
                     ADVGWVTGHSYVAYGPLALGATVVLYEGSPTTPDPGRFWKICQTHAVTIFYTAPTAIR
                     ALMKLGEEWPQRYDLSKLRLLGTVGEPINPEAWMWYHRVIGRERCPIVDTWWQTETGG
                     ILISPLPGVTATKPGSCTKPLPGIEADIVDERGKPVTRPEAGGYLVIRKPWPFMLRTL
                     WRDNDRYVAAYWEKFNRRYYVAGDSARRD"
BASE COUNT         3689 a         6884 c         6894 g         3625 t
ORIGIN      
        1 ttcagctcat cgagccgggt gatcggacca atgcgtacgc gatgccaaac atccgcatcg
       61 acggcaacac gctgcacctt cgcatcgatg ccttgcatcg cgagctgggc acgcacccgg
      121 tccgcatccg actcgttgcg ataggagcct gcctgaagca cgtagacgcc gggccgctcg
      181 accggagccg ccgtgggcag atcacgcttc acctccttgt ccttctccgg cacgaccacc
      241 tcgaaattcg gcagcatctc gtagaagtcg tatttctctc cactgcccgg cgtcgcggcg
      301 gcctctgggt cggcgggcgc ctgtcggtgg ggatcagggc gaggggcttc ggtcttgtcg
      361 tgctgcgcct ggtggcgact gctcacgtag tagacggcgg ttgcgccgag cactacgccg
      421 aaaaaggcac ccacggcgaa ttcgcgtgcg cggccgagct gaaccgagcg cctgtgagat
      481 ttcttgtagt cccgcgtgtt gagcctggcc attgccgcgt agagcgtttt cgttcagtta
      541 tctaggctgc gctgttgcag atgacacgcg aggaccctcg cgcgctgacc tgaggcgatt
      601 cacatggtct ccggcgcagt gacaccgagc agagtaagac cattccggat cacctgctgg
      661 accgcctgga cgagtgcaag ccgtccgtta cgcagcttcg agtctgccac gatgaactgc
      721 tctgcgttgt agtacgtgtg gaaggcgttg gcgagctccc gcagataatg gaccagcgcg
      781 tgaggggcgc gagaggtcgc ggccagctcc agcacctccg gatagcgggt cacggcacca
      841 agcaccgcct gctcatgctc tttggagagt ttgtcgatgc tcgcgatacc ctccgcgaga
      901 tcgaaggcga agccacggct cgcgagctgc ttcatgacgc tcgcgacgcg ggcatgcgcg
      961 tactggatgt aatacaccgg gttgtcgttg gtgcgcgatt tcgccagctc gagatcgaag
     1021 tccagaggct gttcgtggct gcgcatcagg tagaagaacc ggcaggcgtc gttccctact
     1081 tcagcccgca gctcacgcag cgtcacgaat tgtccttcgc gcttgcccat ggcgaccttc
     1141 tcgccgccgc ggtacaggct gacgaactgc accagattca cctccaggga atcgcccggc
     1201 tcgcccatcg caacgagccc ggctcggaca cgcgccacat agccgtgatg gtcggcgccg
     1261 agcacgtcga tcagccgctc gaagccgcgc tcgcgctttt ccagatggta ggcaatgtct
     1321 gacgcgaaat acgtcctgac gccgttctcg cgtaccacga cccgatcctt ctcatcgccg
     1381 aactcagtgg cacggaacca ggtggcgcca tccttcgcgt acagacggtc ctgttggcgc
     1441 aggcgctcga gtgcgcggtc gatggcgccg ctctcgctca gagcgcgctc ggaataccat
     1501 cgatcgaagc gcacgccgaa ctgctccagg tcggtgcgaa tgtccgccag catctgctcc
     1561 agcgacagct gaacgaggcg atggaagtca tcgctaccca ggagctggcg ggcgcgctcg
     1621 atcagggcat caatgtaggc ttccttgtcg ccggcgggct cgtccgcggg aagatgttcg
     1681 agcaccgccg tggcgggccg ctgaaatcgt tcgcctacct ggaggcagac gtagcgggcg
     1741 atcgcacgga tatattcacc gcgatatcca tttgcgggaa acggcaggct cgcgcctttc
     1801 agctccagat agcgcagcca cacactgacc gcgaagatgt cgacctgtct gccggcatcg
     1861 ttgatgtagt actcccggct gacccgaaac cccaccgcat ccaggatgtt ggaaagcgtg
     1921 gcaccgtaag cggcctgacg gccatggccc acatgcagag gaccggtcgg attggcggag
     1981 acgaactcca ccatgaccgg ctgtgccgcc cccagtgtgc tctcgccata gcggtcgcct
     2041 ccttcgtgta tgcgcgcaag ctcctgagag taggcctggg ggctgaggaa gaagttgatg
     2101 aatccggcgc ccgccacttc cgcccgcgag acgagcgcgt tggccggcaa cgctgcaacg
     2161 atggcttgag ccagctcgcg cggactgcgc ttggcgcttt tggcgagccg caaggctacg
     2221 tttgtggcga agtcaccatg ctgcgattcg cgcgtccgtt ccacgctgat agcagccggc
     2281 tcgatacctt gggggaggac gctgcccgcc agcccattca ctgctgtctg caggagttgc
     2341 tcgatctgct gtttcaaagg cccgaaatgt ccgtccgcag aaaagggggc ggcagtttac
     2401 cgcaaggtcg cgcgcagccg cccgctgccg tcggggtccc cacgaaagcg agcgtcttgc
     2461 tgatcctcga gagccctcgc acgtgtcgat ctcgatttcg tggggtggcc tcgcagggcg
     2521 actttttccc tgcgctggcg caggccgcaa aagtcacgtc tttgcggccg cagtacaaac
     2581 cgatgtccga aaaacgccgc aggcgacttt cttcaatgaa atcagaatag ctccagggga
     2641 tccacgtcca gcgcccagcg gacccgtcgc gagctcggga gcgcctcaac ttccggcagc
     2701 cagtgctcca ggaatcgatg cagggcgctt cgctcgggac tctcgatcag cagctgagcg
     2761 tgatgccgac cggcgcgcct ggccatcgca gccggaacgg gtccgagtac ctgcactccg
     2821 gaaggaggct gtgtgagctt gcgggcgtcc gtcagaaacg cgagtgcgtc agaggctgtc
     2881 tgcgccgagg ttcgcaacgc cgccagccgg ctgaagggcg gccaggcggc ctgggcgcgc
     2941 tctgcgaggg cggtccgggc aaagccgtca tagccttccg tgagcagact ttgcaacatg
     3001 ggatgctcgg ggaattcggt ctggatgagc acctcacccg gccgcgccgc gcgccccgcg
     3061 cgaccggcaa cctgcacgat cgtctgggcc aggcgctcgg gtgcgcggaa gtcggtgctg
     3121 aacagcccct gatcggcatt caacaccacg accagcgtga cattcgggaa atcgtggccc
     3181 ttcgtcacca tctgcgtccc gatcagaatg cgcgcctcgc ccgaggccat ccggcgcatg
     3241 gcctcctcga gctcgccgcg cctgcgcacg gtatcccgat cgaggcggac tcgcgccacc
     3301 tgcggaaaca gggcggccaa agattcctcg atccgctcgg tgccctggcc aaccgaccgc
     3361 gtggcaaagc cgcactgtgg gcatcgctcc ggcagcggcg catcggctcc gcagtgatgg
     3421 cacctcagtc gcccggcgga cagatggacg gtcagtcgcg catcacactc cgaacaggga
     3481 gcaacccagc cgcaggccgt gcagaggagc gtcggcgcgt agccacgccg attgagaaag
     3541 acgaggacct gtccgtccgc atcgagatgc ctctgcactg cctggatcgc ctgggtcgag
     3601 ataccggcgc gcacgggact cgttcgcagg tctatcagag ccatgcgagg cgcctgcgcc
     3661 tgctccgcgc gccgcggcag ggtcagccgc gcatagcggc cggaattgac gttgttcaag
     3721 gtctccagcg caggcgtggc cgagccgagc agcaccggga cattggcctt ctgcgcccgc
     3781 agcacggcga gatcccgcgc cgaatagcgg aatccaccct catgctgctt gaacgaagcg
     3841 tcgtgctcct catcgacgat gatgaggccc agctcgccga ccggtgcgaa cacggccgaa
     3901 cgtgtcccca acacgatgcg agctcggcca ctgtgggcct ctctccaggc cagcaggcgc
     3961 tcgtggtcgg tgaggccgga atgcagcacg ctcatgggcg cgtaaaagcg ctcgcgaaag
     4021 cgtccaacga gttgaggagt caggccgatc tcgggcacca gcaccagcga gcgctcgccg
     4081 cgggccagca cacgctcgac gaggcgcagg tagacctccg tttttccgct gcctgtcacg
     4141 ccgtgcagga cgaaggcacc gaagcttcca agcgagttgc cgacactctc caccgctatg
     4201 cgctgttgct cggcgagcgg gggcccggac tcgcgaactc caaggggggc ctgcggcgta
     4261 ctgggggata tctcgagcga tccggcccag ttgcgcgcga ccagggcgcg agctgcggag
     4321 cgccatcccg ggagctgttc tgtgagaacc tccgcactga cagtctcgtg atccaccagg
     4381 taccggagca aggcgcgctg acggggcgag cgccgtggct cgcctctcgc caaggcttcg
     4441 tgaccttcgc cggtcgcgca ccacgattcc tgcaccgcgg ttgcgcgcgc cccgaggcga
     4501 agcgctttgg gcagggccgc cgctgtgact tcgcccagag gatggtggta gtactctgcg
     4561 gcccaccgga gcagctccag cgcggtggag tccagaacag gtacgggatc caggatctcg
     4621 atgacggatt tgagccgctc ggggggcagg tcggatgtgg gcgtgacttc catcacaacg
     4681 ccgatcagcc gctgtccgcc aaagggcgcc cgcacgcgga cgcccggtgg gacctcagat
     4741 agcgcaactt ccgccccgcc gggggcggca cgggggggca aataatcgaa cagacgccgc
     4801 agcggcacgt cgaaagcgac ccgaaggacg cgctcgacgc tcatgggtac ttgtaaatca
     4861 atgggttggg cgtgcagcag tgagtcaacc ggccgtggcg gctgcgcgtt atacatgcca
     4921 tgtccgggtt tgctacgctc gcgccgcctt atagcatgcc gtcggcatcc cacggcgacg
     4981 tgaaactgtc ggattcgatc tcaagcagcc aatcgcgtgc gctgaaccag tttctggccg
     5041 gcgtcgagct caaggctttc aagattgcgc aggcggcact gcgcaacgag gacgacgccc
     5101 tggatgccgt ccaggatgcg atgttgcagc ttgcgcgcgc gtacgctgac aagccggcgg
     5161 aagaatggaa gccgctcttc tatcgcatcc tcgagaatcg gatccgggac atgcagcgcc
     5221 gccggacggt acgaggccgc gtgatttcct ggctgccgct gcgcagcaac gacgaggacg
     5281 aagagcccga tccgatcggc caggcaccca gtgtcgagcc ccagccgccg cgccggctgg
     5341 agatcgccga ggcgatgggc gccctggagc gtgcactgca ggagctgccc cgccgacagc
     5401 agcaggtgtt catgctgcgt accctggaag gcctcgacgt ggccgaaacg gcggcggcca
     5461 tgggttgctc tgaaggcagc gtcaagacac attactttcg cgccctgcag agtctgcggg
     5521 cgcaactcgg agagttctgg acatgagccg gatcgaagaa caaatgccag gcgagctcga
     5581 gcgacggctg aagggcctcc tcgaagagag cgtggcgcat gttgacgggc gcatccgctc
     5641 gcgcctcaac caggcacgcc atgccgcgat cgttgaagca tcacggccgc gccggcctgc
     5701 attgctgcgt cccttcgtgt tgatgccgac cgcgggagcc ttggcagccg cggcgctcgt
     5761 cgccttcgtg ctgtggccgc attcccccca ggtcgaaata ccgatgacgg ctgaagccgg
     5821 ccattccatg gaggacctgg atctactggc cgatggcgat gggctcgatc tggtttcctc
     5881 ggaggagtcg gacggctcgt tcttcgagtg ggcggcggat cagaccgata ccaatgagac
     5941 gagcacctga tggcggggcc tggcgtccgc gtgctgctgg cgctggctgc attgacgccc
     6001 gcgtgcccga ccgtgctcgc agataacaag gcaccgcctc caccaaccga cgtcgagttg
     6061 ctggaatttc ttggcagcgt ggattcggga gcggatgcgc agggcgcgga tgatggaagc
     6121 tggatcgact acctctcgca aaccgatatc ggaaaggccg cccgcccggc cgatccgaac
     6181 ggccctgcgc gggcgaaaca ggtcgcctca ggcacccagc cgccgtcggc cggagacaag
     6241 aaagatgaat aagcggcaaa cgctcctcct gacgatcctg ttcgcggcgg cattaccggc
     6301 gttcgggcag gctgtgcccc ctccgccgag cgttcagggg ccttcgagcc cgccggcggc
     6361 gcagagcgtg ccctggtcca gcctctctcc gcagcagcag cagctgctcg agaagtttgg
     6421 tgtcaactgg aatgcgctcg ctccggagcg gcagcaggcc ctcgcgcgtg gcagtcagag
     6481 atggctcggc atgacgcccg agcagcgggg attggcgcag cagcgctttc agcgctggca
     6541 cagtctgccg ccggatcaac ggcgcgcgct gcgcgagcgc tgggaaaagt ttcagtcact
     6601 gcccccgaag gagcagtcgg cggtgcggca gggtttccgc cggtttcaac agctcccgcc
     6661 cgatagacgc aggatgctgc gcgagcaatg gcgaagcgcc tctccggcgc agcgccagca
     6721 catggtgcaa caggcgcggg agcacaggat gcagcggatg cagccgcatc cgccgccccg
     6781 tcgctgagct caggatgcgg gtgtgcccga acgcgggcgg ctggggacgg ggacgttgca
     6841 gacatcaacg cggcctgcgg gtaccttgaa ccattcctcg cgccgattgc gtcgcgcctc
     6901 cacgtaagcc gcaaacgtcg gtgtgtcggt ccgcaggact tcgagctcgg tgcgctccgc
     6961 cggcgggaca tcggccgcta cgcggatgga tttgatcggc aggcgttgct cggacttctc
     7021 atagaagccc agcgcacccg tgccgcgcgg caaagtggag agcagctcca tgccctggac
     7081 gacgcggccg acgagcgtga cgttccgatc caggtgacgc ggagcctggc cgatcacggc
     7141 atacagctca gtacctccgc cgctgtccac atcgttatcg cggcccgctc ccaccatgcc
     7201 gtagcaatgc accagccagg cccgaccgaa cttgggatcc acggccgcag gaaagccgtc
     7261 cgaaaagccg acttcggggg cgtaggtgtc ggggtccgga agaggcgtaa acggcacacc
     7321 ccgtgagggg cgctcgaact ccgccgccag ggtgcgctgc gcgttcttga ccgatcgctt
     7381 gccctcggga tcggcccatt gcacgacgta gttgtcctga gcgcgcacga tggcgagtcc
     7441 gtcgtagtag ccctcgcgtg caagagcttc gatattggcc acatgatgag gcgcgaactg
     7501 gggggccagc tcgatgatca cccgcccgga aggcaactcc atgtacagcg tgtgccgctc
     7561 atcaagcggc ctccaatccg aagctgtgga ggcggcgatc acgctcgccg tcgtgggcgg
     7621 cttgcgcgca gtctcggccg tcttcgtcgg ggcggccgcc ggcttcggcg gggccgcggc
     7681 tgctggagtc agaagcggac agagcagcgt cagtgccact gcgagcggcg agagattccc
     7741 tggggtcttc atgatcgggc ggcgctcctg ccggtttatc ccactgcttt gatcgcctga
     7801 atgagctccg cccccgcctt ctgcgcatcg ccgtacagca tgcgcgtgtt gtccagatag
     7861 aaaagcgcat tctcgatgcc ggagaagccg gcacccttgc cgcgcttgat cacgatcaca
     7921 ttgcgggcct tgtcggcatt caggatgggc atgccgtaaa tcggactcga tttgtcgttg
     7981 cgtgccaccg ggttcaccac gtcgttcgct ccaatgatca gcgccacgtc cgcggtctcg
     8041 aactcggagt tgatctcatc cagatccgag atgagatcgt aaggcacgcc cgcctcggcg
     8101 agcagcacat tcatatgccc cggcatgcgc ccggcgaccg gatgaatggc aaatttcacc
     8161 gtcacgccgc ggtcgatcag cagctggcac agctcccaga tcttgtgctg cgcctgcgcg
     8221 acggccatgc cgtaccccgg caccacgatc accttctgcg caaaggccat catgacgccg
     8281 acgtcggaag cttcgatggg cttcaggctt ccggtgacct caccggtggg tgcgccgcca
     8341 gtgtcgccga agcctgagaa cagcacgttg ccgagcgagc ggttcatggc tctcgccatc
     8401 aactgtgtca gcagcgtgcc ggcggagccg accaccgtgc cggcgatgat catcgccgca
     8461 ttgttgagca cgaatccttc gaaggccacc gccaggcccg tgagggcgtt gtagagcgag
     8521 atcacaacgg gcatgtcggc gccgccgatg ggcagagtca tcgtgacgcc gagcagcagc
     8581 gccaaggcga agaacgcgct gacgaccgcc ggtgctgcat tcagatgcaa ggcgaccagc
     8641 gcgcccgcga tcagggctac gagcagcagg acgagattga agatctgctg gcccttgaag
     8701 cggatgctgc gcgtgatgag gccctgcagc tttccgaagg ctattgcgct gccgctgaag
     8761 gacaccgcac cgatgattcc gcccaccacc gccagaatgg agatggcgta cccctggctc
     8821 tcgcctcgat acagctccac cgctgcaatg gccgcggcgg cgccgccgcc catgccgttg
     8881 tagagcgcga tcatctgcgg catgttggtc atggcgacac gcttgccgct ccaccatgcg
     8941 agcagcgagc ccacgacgat tgccgggatg atgagctcgt agttgctcat ccccgggaag
     9001 gtgaaggtga cgagcgtggc gaccagcatt ccagcccccg cccaccagat gccgctgcgc
     9061 gccgtgacgg gcgagctcat gcgcttgagg ccgagaatga acagcgcggc agtgacgaag
     9121 tagctcgcct ggatcagggt gttggcaccc aggggagcgg catccgctgg catcagtgcg
     9181 cccctttcga ggagccgggt ttggcgccgc gtgcgccgct cgacttgaac atctcgagca
     9241 tgcgctcggt gaccacgtaa ccgccgaccg cattgccggc ggccagcagc acgcccacga
     9301 tcccaatggt cttctccagc ggcgtatgcg ctcctcccag agcgaccatc gcgcccacga
     9361 gcactattcc gtgcacgaag ttcgatcctg acatcagcgg cgtgtgcaat atgaccggca
     9421 cgcgcgagat gacctcgtag ccggtgaagg ctgcgagcat gaagatgtag agcgcaacga
     9481 tacccgtcat gtgctgcttc tccccggtgt tggcatcaca gttcccccgc aacgagtgcg
     9541 cggcgtggcg ggctcgtccc gtcagccccg cgcagacctc gggccccggt gccctcagcc
     9601 ctcgatggcc ttgcgtgtcg cctcatgttt gatctcgccg gaatgcgtga ggcacgactg
     9661 cgcaaagacc tcgtcgttcc agtctatggt gagctcgccc ttgttgaggg caggcttcag
     9721 aaagttgagc aggttgcgcg cgtacatctc gcttgcgttg taggcgagcg tcgcgggcag
     9781 gttcaccgcg ccgatgactt tcacgctctg atggtccacg acttcaccgg cgcgtgtgag
     9841 ctcgcagttg ccgccctgct cggcggcgac atccacgatg acggagcccg gtcgcatccg
     9901 ctcgaccgcg gcgcgcgaaa tgatcttcgg ggccgggcgg ccgggcacgg aagcggtcgt
     9961 cacgaccgca tcggccgcgg cgatgcgcga gtccagaact tcctgctgct tcttcttttc
    10021 atcttcggtc agctcgcgcg cgtacccgcc cgtgccctcg gcgctgactc cggtgtcaac
    10081 gaactttgcg ccgagggact tcacctgctc tctggtggcc gagcgcacgt cgtacgcctc
    10141 gaccaccgcc ccgagccggc gtgcggtggc gatcgcctgc agaccggcga cccctgcccc
    10201 gatgaccagg accatggacg gacggatggt accggcagcc gtcgtgagca tgggcaggaa
    10261 cttctgcaga tgattggcgg cgatgaggac tgccttgtag cccgcaatcg ccgcctgaga
    10321 ggacagagcg tccatcgact gtgcgcgcga gatgcgcggg accagctcca tcgcgaagct
    10381 tgtgatgccg cgctccttca gcgccttgac ttcctcgcgg cgcgaatagg cctgcatgaa
    10441 cccgacgacc accgcgccct tcttgagctg ctggatctgc tgcagcgaga gcggctgcac
    10501 cgtcagcacc acctcggact gctcgagcac ggcggcagcc gagtccgccc aagtgacgct
    10561 cttgaacgag gcgtccggaa actgcgcccg cactccggcg ccccgctcca tgaggatgcg
    10621 ggcgcccgtc tgggtgagct tgtcggccac ctcgggaacc aggctgacgc gcgtttcctg
    10681 gggcgtgctc tcgcggagcg caccaatcgt gactggcatg cagggccctt ccggcgtgtt
    10741 tacgtgttct ttcgtggccg gctggccacg ctagcggaaa tcctcaagcg aattaagcgg
    10801 ttgcaacatt gaattctggc acaattctgc tttatcttac agcgcccatg cttggcatag
    10861 atctcgatcg agccgacttc ggcttccagg acgacctgcg catcgtcgct tctcccgcgg
    10921 cgcggggact ctcgcagctc gttttgcgta ccgatgtggc cggcatgcct ctcgagtgga
    10981 tcgactacaa ggaggccgca cggctctacc accaggaaca ggtagcctat acgtgcggct
    11041 cgccgctcta caagctctat ggcgggatca atgccgccag cggattgcgc accgtgctgg
    11101 aggtcaactc catcgtggcc accgttggac acaccggcaa tccgggcaac acccgtcacg
    11161 actacatccc gccgctcaac aatcagacgc tcttccggcg ggatgcgtat ttgtgcatgt
    11221 actgcagcat gcgatttgct tcgcgcgagc tctcgcgcga tcacatccga cccttcagcc
    11281 agggcggcct cgatgtgtgg accaacgtgg tgagcgcctg ccgacgctgt aacaatcaga
    11341 aggcctcccg cacccccgag caggcccgga tgcagctcat cgcggtacct ttcacgccaa
    11401 cctacgcgga gtacatcttc ctgaagggac ggcgcgtgct ggcggatcag atggagtatc
    11461 tgctggcgca ctttccgcgc tcgagtcctc tgcacgaccg tattcagcgc tggctgtcgt
    11521 gagccgggct cacctgagga gtcggcggca gccgggcgaa gccgcggtcg gggccgagtg
    11581 cgggtctgat cttcagggcg gcgccgaggt ccgaagttga tcttcctgat agacgcctcg
    11641 gtctacgtct tccgtgctta ccactccatg ctgcctgaca tggtcgatcg cgatggtaat
    11701 cccgtgcacg ccgtgtttgg ttttgcgcgc ttcgtcggcg acctgatcga gcgcgtgcgc
    11761 ccgcagtaca ttgccgtggc gttcgatcaa cggctcagca actcgtaccg cagccgcatc
    11821 tatccgccgt acaaggcgaa tcgtgagccg cagccgttcg gcctggccgc gcagttcgag
    11881 tgctgccgcg aggtctgccg ccatctcggg cttgccgtgt tcacgcatcg tgaatacgaa
    11941 gcagacgatg tcattggcac actggcctgt ctgatgcgcg aagagggaat ccgctcagcc
    12001 ttcatcacgc gcgacaagga cctggcgcag ctcgtgcgcg atggcgatct gttctgggat
    12061 tacggcgcac gtgagcagtt tggctaccat gacatccagc ggtactacgg cgtcgctccg
    12121 gaaagattcg ccgattacct ggcgctgacg ggtgattgtg tcgacaacat tcccggggtg
    12181 ccgggcgtcg ggccgaagac tgcagcggcg ctcatgaagg agttcgcgtc cctggacgag
    12241 ctctacgatg gtctgggacg catcgcgcag atgcctctgc gcggagccca atcgctcgag
    12301 cagcggctgc gcgagcatcg cgagtcggta taccttgccc ggcagctgac gcgcatcacc
    12361 tgcgacatgg agctgggtgt aggcgtcgaa ggcttgcgcc ggcgtcctcc ggacatgccg
    12421 gctctgtcca agctctacga tcgattgggt ttcgggccgt ttctgcgccg ccagggcgag
    12481 cggcttgcgt tgctacctgg gccgagcgct ccttcccgtt cgctactctg actcgagcgc
    12541 cgcgctcgtc aggcagccga tgggttcccg gctgccatcc ggacgacgcg cctcgaggtg
    12601 cacgcttccg tcgcgcaaca cctgcgcatc gaggcgcaga aggcggctcc catcaggctg
    12661 agtgcgcgcc gagtactgtg atgaggacag ctcgcgacgg ttcgaatcga gcgcaagcaa
    12721 ctgctgatcc cgcggcaggt taggtatcct cagaactgcg gactgtgtgt ccgttgcaag
    12781 atgcaacagc gtgcagtctg ctgcgcgtcc ggtgtcggcc atactgaaca ccgcggtgtg
    12841 cgtggaatcg aggtcggtca cctgtgtggt cttgccgacc catccgccgg ctgcacccac
    12901 cacgccggcc cccacgagcg aggcggccat gcgccagtcg gaaaaaaccg gctgccgcga
    12961 cttgcgcggc gtgctacgcc cggaaggctg agcccgatgc atatcccggc gaatggcgcg
    13021 ccagatctcg acatcaccca gacactcggc acagcccatc atgtgcagct cgaacgcctc
    13081 cagggtgggc gcatcgagcc catcggcgac gtagctcgcg gccgtctgat tcgccttgaa
    13141 ctgtatgtgg tccatggtac tccttacctc tcggtcgatt gcgcagtctc cgactcgcca
    13201 gctgcgagtc cgtgcctgcg caggatgtcg ccgaatcgct ggcgtgcgcg ccagaggacc
    13261 tgattgaact gcatgtccgt cagttgcagt gactcgcgga tggccgtccg gggctcttcg
    13321 tgaagataga accgctccag cacttcgcgg tcgcgctgta caggcagatg gctcaccagc
    13381 tcgcgaacac acttggccag ctgctcctga tcgagacgct cctcgagcga gagggctcca
    13441 tccttgagac tggaaagcaa ctccgcatcg ctgtcgtgcc ggtgtgaaag ctcaccgcgc
    13501 cagtaagcga tcgcgagctt gcatgccgtg cgatagagat agcctccaag attgcccggg
    13561 tcatccagac cctccgtacg gatcttcttg atcgcttgca aaaacgtgcg ctgcaacagg
    13621 tcctcggcgg catcgcgagc gatccgcctg tgcaggaata cctgcagcaa gggcctccga
    13681 tatctcacgt aaagttgatt ttggaggctg ccgcgtccgg caagcgcttc caggagtgac
    13741 atatcgccct gctcggcgcc atcagcgggc ttttcccgac aaagctcggc gggaggcgca
    13801 acgctatatg tcatggatgc gcttcccgtt gcggcactgt ggcgtggcaa acacgcgcgg
    13861 ttcggccgtc gccttccaac agacaagcgt cggccaacgg gaccccttct cggtaggtta
    13921 tccgcgctcc gggcttggaa cggcgtggag tcttcagcat gcgtccttgt acgaccctgg
    13981 ccaggaactc gtcggtattc aatttagcat agcgcgggtc cgcccagatg ccagccagaa
    14041 gcccggcgag gatggctgtg gaattggagc ttccctgttc cagccggtat tggtcgggcg
    14101 ggcccccggc cccgatcacg catgcactgg gggaacccag attcagccct gccggagccg
    14161 gactggtccc tggagagatc gtgtcctcat tgccgatcgg gatggcgcgc acgatccggc
    14221 ggaaccggta ggcggccgga tagcccgcgc tcggctcgcc cttgttgggc acgctggcca
    14281 cgacaatcgc cgccctcggc gaatccggac ggtccgcgat atcggcgaag gcggcatcca
    14341 gctcgggcgt cgttgcattc cagtgggcgc tgaaactcag gatgcgagcg cccatgcgcg
    14401 tggcatagtc cagcgcccgg atcagccgct gcggacttgc ggtgtcatcg acaccggaat
    14461 ggcaaccaac gacccgaaga atcatcaccc gggcggcatc ggacccgggt gccgcgatac
    14521 caacgccgtt gttgcgttcg gcggccacga ggctcgccat gagagtgccg tggctgaatc
    14581 gggagcggca ctcgcccttg ggcgacacat cagggctgtc gtctacgaaa tcccacccgt
    14641 gaacatcgtc gacataacca ttgccatcgt catcgacgcc cgccttgccg tcagcttcag
    14701 cctcgttggt ccacagctgg ttgcgcagct cttcgtgatc cacataggcg ccatcatcga
    14761 acacggcgac gatgacgggc tgtgtgggct tgcggtcacg ccaggccagg gagcccgcaa
    14821 catagtcgac ccaccacagg ttgccgaccc ggtatgcgca gtcgttcgtt ccgacatcgt
    14881 cgacccgcga gggcagcaga gtggagcagg tcaggggccg gcacggaaat ccggccggct
    14941 cctgcgcgct cgcggaaacc acgcttgaaa gaaggcagca cagggtcgtg gtggcgcggg
    15001 gacggcgcat gccacaattc taaacaagcg cgctcgaggg atccggcact ggatcccctc
    15061 gtcccgaggc gaatccggac ctgagtcacc aggtggccga gccggtcagc tcgacgcgca
    15121 gcccgcccgc ggaccgccct gcggcccggt ggaagcgcat tcgcacgagc ggcatcggcc
    15181 gcaggtaggc gtattccgca cgccgctgcc agcgacagac ggcggctgga ggcggtggcg
    15241 cgcccccgcc gccgtgcccg ggggagccaa gtcagtcgct gctgtggatc acaccgtcat
    15301 cgtcgagctg gatggtgctg ggcgctgtca cggaggcgag cacgatgatg gccgaggagg
    15361 cgcccttcat gttgcgcggg aagatcagca cgtgctcgag ctggccgtgg atcgtcgcca
    15421 ggccgtacca atgctgttgc tcgtagtagg gctcgctggc ctgggcgtag cccgacatgg
    15481 cgagaaacgg cgagctcgat tcctgtcccg gaatgcactc gaagtcggcg gggacgctgg
    15541 tcatttgtgt gccggggcga tattcgagcc gcagcccatt ccagttggcc gcccactgct
    15601 gctgggcgcc gtcctcggcc tgaaaacgat agctgccgtc ggctgccgcg gagtcccggg
    15661 aaatgcgaaa tcgcaacccg gcagcgacct gggcggcaag acccgcgtgt gcttccgtgc
    15721 agttcttggt caccgtccat tcccggaaga aatactcggg aatctgggca gcactggcgg
    15781 ggccagccgc ggcgaaggcg gcaaccagac actgtccggc gatggacgcc gacagggtcg
    15841 ctttttgagt cagggacatg gggatctcct cgggtgggcg gtgcgaatcc gctccagttc
    15901 tctgctgcga ggaggccgct tatctcagcc gtgcaggctg cttgccgccc ctgccaggcg
    15961 cgacacccga gagtcagatg ctgctgtcgg gccagccgta caaggcaaac gccgcccatg
    16021 cataagagga ggagctggag gcccgctcga gctgcgcctg tcgcagcgcg cgggaggccg
    16081 gcaatcgatc gagcagcaag tgccggtaga actgctgcat gaaagctgcg gtggcctgat
    16141 cgtccacacg ccaataactg gcgatgacgg ctgcagcgcc tgcctccaga aatgcctgtg
    16201 ccgggcccaa tacgccttca ccgggaagca gtcggccacg cgaagtgtcg caggcgctga
    16261 gcacgaccag gctcgaattc aacttcagac cgaggatgtc gagcagcccg aaggcggagg
    16321 aggcttcatc gcccgcttcg ggggtcaacg ccagagccga gaggcgcggg cgctgcgcat
    16381 cgacgcgtgc atgagttgca atgtggagca gctctgcact ctcggctccg cgccggaggg
    16441 cttccgggac tgctgcacaa cccagcgcca ggtcaacgcg agcgccaggg cgtgcctcct
    16501 gcatcagttt ggcgatcgag agaacttcgc tgcgactgcc agggagcctg ggaagagaat
    16561 ccagccaatc cacagcggag cgcgtggcgg gaggggcagg tgaggcttcg gccccgccat
    16621 tcaggcattc gcgtcgccag cgggtgaggc caaagacagg atccccgatc aacttgacgc
    16681 gtggggcgtc gtcgcgatgc gaaccccggt cgggcctgcg cagcagaaat cgcgcggagg
    16741 ggagtactgt gacctccgca tgctgaagca caagctggtc cgagtcagag gcggaccacg
    16801 gcagtacggt gaagggcacg gtgtgcagcg catcatcagg cacgaacacg atgcgcttgc
    16861 ctgagagtgc cgcgtgcacc ggccagagaa ccaaccgcgc gagcttcgta gccgcttggc
    16921 gcacggtgtt ggctggagtt tcaggatcgc gcaatcgctc gagcagctcg gcggatgccg
    16981 tcgcaatctc gcgccgcccg gcaagccgga tcacctggac tgcgccgtcg tgcaccacga
    17041 aggcaaacat gtcctgcgtc cccgctgcgt attccaccaa ggtggcactc agctcatcga
    17101 gtgtgcgcag cagcgactcg cgatcgagcg gctgagtggc gccgcgctcg tgctgtgcga
    17161 ggttgtcgag ctgctgcacg aggccctcag cgggctctgc ggtatcagca tgggtgagct
    17221 gtgcagcttc gcgcaacatc tccctgtaac gcgccgtcgg gggtggcgcc gcagagtctg
    17281 cggccgcccg cgtcgcctga gtcaccgcgt atcgcaggct tcgagcgcgg ccgcgttcgg
    17341 aggtggaaaa ggctctccac gcgaggtccc gatccgtttc ggcctgcgat gcaaacaggt
    17401 ccgtgagatc ggcaaacacg ctgtactgtg tggcgaggta ggtcgcgcgc ttctcgccat
    17461 cgagctgatc gatgggaacc gcctcgatga gatcggcggc cgtcagcaaa gtgcgggccg
    17521 cctcctgcgg ccgactgccc cggtacgccg cggcgagcag tcgcagggag gtcactgttt
    17581 gatcgacatc gccgcgcagc acactcgagc gcaaggtctg ataggactgt cgcatcgcgt
    17641 ccccggcatc tccggccagc agagccgctt tgaccatgcc aagacgcgcc gcgccacgat
    17701 gctcgtaggt ggatgacaac cgcgcgacat ccgcgatgtg caaggcctga ttgaaagccg
    17761 ctcgcgcacc ggcggcatcc gcgcgctcga gcagcatcgc cccgctggca ttttccaatc
    17821 ggacctgggt gcgcgagtcc gctgcataca accgagccgt ctggaagcag cgctgcgcct
    17881 gggagagctc tccctccatc atgagcacgt ttccgagcga cagcagcgag ttgaacacga
    17941 cgatcctgtt ctcactggcg aggccttctg catcgatggg atgcacgcac aagcttcgat
    18001 ggggcgtggc cgcgccagcg gagttgcgct gaggtatcgg gagatcggaa gcggcaggct
    18061 gcgtcaggcc cgactgcggc gtcccagccg ccgacagcag atcagcgcag tcgaatgagg
    18121 ccgcccgctg cagatagctc tcggcatcgc cgacgacacc gagctgtgtc atgagggtgc
    18181 ccagacgtga catgctgcga cgaacacccg ggcagtcacc gagctgcgca tactcgtgca
    18241 tcgctgcttc gtgcgagcgc tggctggcgc tgaagaggcc gacgaccccc tgcagccgtc
    18301 caaggttgtt cgagatatcg gctcgcaacc ggggctcgag cccgaccgga agcaatcgca
    18361 gagcctgcgt gtaggcctgc agcgccgaat catagttgct gctctcctgg agcaacgcga
    18421 gattctgggt ggcgactgcg taacaccccc agtcccgggt tttccgacag tgctcggccg
    18481 cctgggtaaa tgccgcttgc gcgcgggccg gcgcatccaa tgtgtattcg agaaagccgg
    18541 tcataatatc caggcgcggc agctcgcggg cgccggccgg gctcgcactg tcgtagcggc
    18601 gcgcgcctgc gagcaactcg cgcactctag ccccttccgc gcgcggctcg gtgccgggca
    18661 tcgctaacag ggcgctggcg cgcagccccg tgagcaatcc tttggtgatg ggatctgcgc
    18721 gctcgccgta gtcggccagg gcctcggccg tcagcgcata cgcatcacgc ttgcgatcga
    18781 aacgccgata ggccagctcc gcgagcgcct ggcgtgcctg ccccgcgtcg actctgagac
    18841 ccacggcgtc gaacccccgc gccgccttga ggtaggcatc gaaggccgcc tgccaattct
    18901 gtttttgagt ggcctggcca ccctgcgcga aagcggccag cggctccgcc agcgccggat
    18961 cgtcgcgcgg gagcaaacgc gcccgcagac aaaattcacc ctgcatctcc ggagagtcgt
    19021 gcggtcggat gcgcacggta tgcagctgac ctgcgagcgt gctcgtgagc aggacaatcg
    19081 tgccgaagcg ctcgacgggc gaggtcgcct cggtgctggg gcgctcatcc aggagactga
    19141 cgagcgtcag gccccgctcg tcgatctgca cctgcagcgt cccgggcgca ggtgccggga
    19201 tcctcatcgt gatctcgcgc cgtccattta ccggcagaca cccctcgctt acgagcctgc
    19261 tcgcggcgac atgcgaaccg cagccagccg aaaggacgct caacagcatg accgctggca
    19321 cgctgcaggt catccgagcc aagcgcggca gcgacgtgcg gggacatgtc cacatgtgga
    19381 tggcgaaatg gaatcagtcg gcagcgtcct tctacgccat ccgcagcgca cctgccaaga
    19441 cggtgtgtgc cgccgcgtcg gttcgcgttg gcgctgcgat actgtcgatc cccggggcca
    19501 ttcggctacc ctatgcgggc ccgcggccct ggtgtcgcag cctggagggg aatctcatgg
    19561 cgagcaggaa tatcgaatcg gtattgctgg aggagcgctc cttcccgccg ccgtccggat
    19621 tcgccgcacg cgccggtttg aaagccgccg atgtggagcg gatgcagaag caagccgcgg
    19681 cggatccctc gcgtttctgg gccgatctgg cgcgccagga gctctcgtgg caccaaccct
    19741 tcagcgtctc gctggatgac agccacgctc cgaactatcg ctggttcacg gacggacgat
    19801 tgaatgtctc tttcaattgc ctcgacgtcc atcttgcgga acgcggcgag aagacagcgc
    19861 ttgtgttcga aggcgaaggc ggcgacagcc ggcgcctgag ctaccgtgag ctgcatgccg
    19921 aagtctgccg tttcgccaac gcgctcaagg cacagggcgt acgccacggc gatcgagtca
    19981 tcatttacat gcctctggtc ccggaagtca tcgtggcgat gcacgcctgc aaccgcatcg
    20041 gcgcgatcca ttcggtggtg tttggtggct tctccgctcc ggcgttgaag gaccgcgtgg
    20101 tggatacgga ggccaagctg gtcatcacgg ccgatggcgg ttggcgaggc ggcagcgtgg
    20161 tggagctgaa ggccgcagcg gacaaagccc tggcttccgg ctgcccgagc gtgcagcgcg
    20221 tgatcgtgct gcgcaggaca ggcaagtccg tgccaatgga tccggaacgc gatcgctggt
    20281 ggcacgatgt gatcgagggc cagccctcga cctgcgagcc cgactgggtg gaggcggagc
    20341 atcccctgtt tctgctgtac acctccggct cgactggcaa gcccaagggc atccaacatt
    20401 cgagcggcgg ctatctgctg ggcgccaagc tgaccagcag atgggtattc gacctgcgcg
    20461 atgacgatgt gttctggtgc acggccgatg tcggttgggt gaccggacac agctacgtcg
    20521 cctacggtcc gctggcgctc ggcgccaccg tcgtgctgta tgaaggctca cccacgacac
    20581 cggatccggg aaggttctgg aaaatctgtc agacccacgc ggtcaccatt ttctacaccg
    20641 caccgacggc cattcgcgcg ctgatgaaac tcggcgaaga atggcctcag cgctacgacc
    20701 tctcgaagtt gcgcctgctc gggaccgttg gcgagccgat caatccggaa gcatggatgt
    20761 ggtaccaccg tgtcatcggc cgggagcgtt gcccgatagt ggacacctgg tggcagacgg
    20821 aaacgggcgg catcctgatc tcgccgctcc ccggagtgac ggcgacgaag cccggctcat
    20881 gcaccaaacc tctgccgggc atcgaagcgg acatcgtgga tgagcgaggc aagccggtga
    20941 cccgcccgga ggctggcggc tatctggtca tcagaaagcc ctggcctttc atgctgcgca
    21001 ctctctggcg cgacaacgat cgctatgtag cggcctactg ggagaagttc aacaggcggt
    21061 actacgtggc cggcgacagc gcccggcgcg ac
//
LOCUS       DASRHN010000421         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_648489, whole genome shotgun sequence.
ACCESSION   DASRHN010000421 DASRHN010000000
VERSION     DASRHN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648489"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..930)
                     /locus_tag="VFB37_17085"
     CDS             complement(49..930)
                     /locus_tag="VFB37_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO24209.1"
                     /translation="MSDAAVPAGELSRGLWREAGRRLSRNRLAVSAGIVIALITLLAV
                     AGPALSPYAYDSIDWQNLAVPPLSAASHWLGTDRLGRDLFVRTLAGVRISLLISLLAT
                     AISLVIGVLWGAIAGYAGGRTDALMMRFVDVLYSLPYLFIVIILTTVFSRGSLPMLYV
                     AIGAVGWLTIARMVRAQTLALKRREFIEAARATGLTPTAILIRHVIPNLIGPVAVYAT
                     LTIPQMILYEGFLSFLGLGVQEPLASLGSLINDGAQEMQSAPWMLLIPAAFLIVLLLC
                     FTLVGDALRDALDPRER"
     gene            complement(923..1846)
                     /locus_tag="VFB37_17090"
     CDS             complement(923..1846)
                     /locus_tag="VFB37_17090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO24210.1"
                     /translation="MLRYGLRRIAGILPTLLVIITASFFIIRLAPGGPFQQEQTLPPA
                     VRVNLDRAYGLDQPLPLQYLRYLRGLAHGDFGPSFKLRDFTVAELIAQGLPVSAALGL
                     SAMLLSIFVGVPLGAAAALRRHRPADHTVTGFTVVAMALPAFVVGPLLALIFGVYLHW
                     LPVAGWESPFPRYLVLPAVTLALPVIAYIARLTRASLLEVLRANFVRTARARGLGPWR
                     VLWRHALAPTLLPVVSYLGPAIAFVVTGSLVVETVFGLPGTGRFLVQGAINRDYSLVM
                     GMVIVYGTLLLGLNLLVDLLYGWLDPRVRHE"
     gene            2040..>2708
                     /locus_tag="VFB37_17095"
     CDS             2040..>2708
                     /locus_tag="VFB37_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZO24211.1"
                     /translation="MKRILVGIKRVVDYNVRIRVKPDGSGVLTDGVKMSINPFDEIAL
                     EEALRIKERGGAEEVIIATIGPADCQAQLRTGLAMGADRAVHVQADGVIEPLVAARAL
                     LKLIEREQPFLVIVGKQAIDDDNSQTGPMLAALWNRPQVTFASKLELADGKARVTREV
                     DAGLETLEVDLPALVTTDLRLNEPRYVKLPDIMKAKKKPLETLPLASLGVEARQALKL
                     VKFEP"
BASE COUNT          543 a          885 c          897 g          383 t
ORIGIN      
        1 tgcggggtct ggaagctcac ctcgagcctc tcgagctgca ggatgggttc agcgctcacg
       61 cggatccaac gcatcccgca gtgcatcccc caccagggtg aagcacagca ggagcacgat
      121 gaggaaggcc gccggaatca gcagcatcca cggcgccgac tgcatttcct gcgcgccatc
      181 gttgatcagg ctccccaggc tcgccagcgg ctcctgcacc cccagaccga gaaagctcag
      241 gaaaccttcg tagagaatca tctgcgggat cgtgagtgtt gcatacaccg caaccggccc
      301 gatcaagttg ggtatgacat ggcgtatcag gatcgccgtc ggcgtgagcc cggtggcgcg
      361 cgcagcctcg atgaattcgc ggcgtttgag agcgagcgtc tgggcacgca ccatacgcgc
      421 gatggtgagc caacccacgg caccgatcgc cacgtacagc atgggcaggc tgccccggct
      481 gaacaccgtg gtgaggatga tcacaatgaa caggtacggc agcgaataca ggacatccac
      541 gaaacgcatc atcagggcat cggtgcgccc gccggcgtaa ccggcgatcg ctccccagag
      601 caccccgatt accaggctga tcgccgtcgc gagcaggctg atcagcagcg agatccgcac
      661 gccggcgagc gtgcgcacga agagatcgcg cccgagacga tccgtgccga gccagtgcga
      721 ggccgccgag agcggcggga ccgccagatt ctgccagtcg atcgagtcgt aggcgtatgg
      781 gctcagtgcc ggccctgcga ccgccagcaa tgtgatgagc gcgatcacga tgccggccga
      841 gacggccagc cgattgcggc tcagtcgacg accggcctcg cgccacagac cccgagacag
      901 ctccccggcc gggacggcgg catcactcat gccgcacccg cggatcgagc cagccgtaga
      961 gcagatccac cagcagattc agccccagca acagcgtccc ataaacgatc accatgccca
     1021 tcaccaggct gtagtcgcgg ttgatcgcgc cctgaaccag gaagcggccg gtgccgggca
     1081 acccgaacac cgtctcgacg accagcgagc cggtcacgac aaaggcgatg gcgggtccga
     1141 ggtaactgac cacgggcagc agggtcggcg ccagggcgtg cctccacaga acgcgccagg
     1201 gtccgagccc gcgggcgcgc gccgttctga cgaagtttgc gcgcaagacc tcgagcaggc
     1261 tggctcgtgt caggcgcgcg atgtaggcga tgactggcag ggcgagcgtc acggcgggaa
     1321 ggaccagata acgcgggaag ggagattccc agccggccac gggcaaccag tgcaggtaca
     1381 cgccaaaaat caaagcgagc agcggcccga ccacgaatgc gggcagcgcc atcgccacca
     1441 ccgtgaagcc cgtgacggtg tgatcggcgg gccggtgacg cctcaacgcg gccgcggccc
     1501 ccagcggcac gcccacgaag atcgacagca gcattgcgct cagcccgagc gcggcgctca
     1561 ccggcaggcc ctgcgcgatc agctccgcca cggtgaagtc cctcagcttg aaggacggcc
     1621 cgaagtcccc gtgcgcaagc ccgcgcagat atctcagata ctgcaatggc aggggttggt
     1681 ccaacccgta cgccctgtcg agattgactc gcaccgccgg gggcagcgtc tgttcctgct
     1741 ggaaggggcc acccggggcg aggcggatga tgaagaacga tgcggtgatg atgacgagca
     1801 gtgtcgggag gattcctgca atgcgccgga gcccgtatcg aagcatcgag accgcctttc
     1861 ctgccaaagg taccgcagcg tatgtatacc gcgattcagc cggggaccga tatcggccgc
     1921 gcttcggcct gcgacttgcg cccgcccggg cggcccctgc ggccacatcg cggcctgccc
     1981 ccgctatact ggcggccctt tggcatctgg ccgcgcagcc tgttgcggag atcttctgaa
     2041 tgaagcgaat cctggtcggc atcaagcgtg ttgtggatta caacgtgcgc atccgcgtca
     2101 agccggacgg cagcggggtc ctcaccgacg gggtgaagat gagcatcaac cccttcgacg
     2161 agattgccct cgaggaagcc ctgcgcatca aggagcgggg gggtgccgaa gaagtgatca
     2221 tcgcaacgat cggccccgcc gactgtcagg cccagctgcg cacgggactc gcgatgggtg
     2281 ctgaccgtgc cgtgcacgtg caggccgacg gggttatcga gcccctggtc gccgcgcggg
     2341 cgttgctgaa gctgatcgag cgcgagcagc cgttcctcgt gatcgtcggc aagcaggcga
     2401 tcgatgacga caacagccag accgggccga tgctcgcggc gctgtggaat cggccgcagg
     2461 tcacgttcgc ttccaagctg gagcttgccg acggcaaggc acgcgtcacg cgcgaggtcg
     2521 atgcagggct ggaaaccctc gaggtggatc tgccggcact ggtgactacc gacctgcggc
     2581 tgaacgagcc gcgctatgtc aagctgcctg acatcatgaa agccaagaaa aagccattgg
     2641 aaacgctgcc attggccagc ctgggcgtgg aggcccgtca ggctttgaag ctggtgaagt
     2701 tcgaaccg
//
LOCUS       DASRHN010000422        12444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2138095, whole genome shotgun sequence.
ACCESSION   DASRHN010000422 DASRHN010000000
VERSION     DASRHN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138095"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFB37_17100"
     CDS             <1..240
                     /locus_tag="VFB37_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24212.1"
                     /translation="WTQRSDFFVTFDRTSNLDEAALTSLDASLAYNVTKWLAITAEAQ
                     NLTNSKVIEYSDVLSHPRAIYENGRVYFAGVKLRF"
     gene            complement(303..2000)
                     /locus_tag="VFB37_17105"
     CDS             complement(303..2000)
                     /locus_tag="VFB37_17105"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO24213.1"
                     /translation="MITTPHPLVLRAREALARGDLRVAETAAEERLKTAGRDINALEL
                     RALIQQRRGQFGQAARTLDTVIGIDPRADWAFNELVQLFLTHGRLTDADKVARAGLRA
                     NPRNAHVHNLFGTILSQMNDMPSGEWHFRKALELAGPHAAVLMNLALNLMRQGRTDEA
                     EGYFGQSHELAPQDMKTLAYWSTLHEARGDLARAAELLDRAEAVSSAEQVNLLRANFL
                     ARIGKPAEALGILDAAGALGGEAQLTRGRLHDRLGHYAAAWEDFVHGKAKLHKETPGS
                     DYRCEAVDALFERCKEFFTSDHVALLPRAKPRTGTPQPIFVMGFPRSGTTLVEQIIAS
                     HSAVLAGGELPFADDISKLTGSLFPGSERFPENLAHTWMADCHYAATLFRDYYLARAE
                     QYGLLRATAFFTDKMPFNEIYLPLLKMAFPEAKIVHLVRHPLDVCVSMLSNNMTHGFN
                     CGYRIEDITHHLAAVTALVTHYRSQLSLEVFTLRYEELVADQEGRTRALLDYLGLPFE
                     AACLRFHENRRYAPTPSYAQVTEKLNDRSVGRHRHYEQQLRPHAVRLKSVLSALGYD"
     gene            2194..4470
                     /locus_tag="VFB37_17110"
     CDS             2194..4470
                     /locus_tag="VFB37_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HZO24214.1"
                     /translation="MSAAPSVGAASSVDVIPLPAHVRPGIGRFTIASGTQLSVSGDAR
                     ALQIAEYFAQLVSKTRATGIEVVRATAGRDPAGTILFEIRPQSATADPESYSLDIAPE
                     RIVLAAADARGLLYGAVTLWQLCTARAARSDPIQIPAMSIEDAPRFRWRGLLLDSARH
                     FQSPDFILKLIDWMALHKLNVLQWHLTDDQGWRLQISRYPLLTSVGAWRVPAGAGAAG
                     DIDPGTGRPRLYGGFYTQQTVRKIVAHAALRNVTIVPEIDMPGHATAAIVAYPTLAST
                     PAPPKTVPSDWGVYPNLFNADEQTFSFLENVLSEVLELFPGQYVDVGGDEAIKDQWHT
                     SAHMQARMHELGVTNEQALQSYFMRRIEQWLHAHGRRLVGWDEILEGGLPPRATVMSW
                     RGLDGAVAAARLGHDVVLSPAPVLYLDNRQGSGAGEPPGRGQVVTLEQLYRFDPRPGA
                     LSSEEASHLLGVQANIWTEHIRTEERVQYMAFPRAAALSELGWSRPQDLSWEGFAARL
                     PDELGRYRALGIHASEDAFTVSIEARMAPGGRGVQVQLAKQAAPGEIRYTRDQSNPTQ
                     TATLYTGPLTLPVGGELRAAAFDAGGALTAVTARSLDAGSIARRSSQELKTCTSRLVL
                     NLEDDAPVEGPRAIFLVDILNPCWIYEAVDLSHVTAVKAAVGQVPFNFQLGSARDSIG
                     FDKPASADGELEVRVDGCEGEPIAVLPLTPARASPVVTTLPAARIAPRSGVHDLCFRF
                     AQQGLEPLWVLDWVEPVG"
     gene            4477..7002
                     /gene="ptsP"
                     /locus_tag="VFB37_17115"
     CDS             4477..7002
                     /gene="ptsP"
                     /locus_tag="VFB37_17115"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZO24215.1"
                     /translation="MDATSSRLTLVSPLQGWCAALEEAPDPVFAGRMLGDGVAIDPTG
                     NSLYAPCAGTLITVPASKHALTLQAENGIQVLLHIGIDTVALAGEGFELHVRAGQPVR
                     AGERLITFDLDLLARKARSLLTPVILTGGTDIVVSRRCVGRRVEVGEFLMEATGAPAA
                     VITAGSASSALEDSVVVQSEHGIHARPAALLAEAIRKLSAEVSIAVRGRTANARSPIA
                     LMSLGIRHGERIAIRASGADSAAALAAVKRTLEARLPEAARSTISAAGSDPPQKAPAE
                     GAAPEQRAREVTGVIASPGVAVGYAYQLARIDLEVAETAPDAARERFALENARAAVRT
                     ELQRTLDREQGAPRTIAEAHLGLLEDPELLERAAKLISLGKSAGFAWRAALREAAEGL
                     RGLEDSRLRERADDLLDLESRVLSMLSGSAAESPELPADSILMGDELLPSQLVALDAH
                     RLAGICVARGGPTSHVSLMAMAMGVPMLVAAGHRILQVATGTAVLLDAESGRLHIGLA
                     EQEIEAARRRIAGQRARHAAALAAAQAESHTADGVRIEVFANIGSTLDAQLALQNGAE
                     GCGLLRTEFLFLERQSAPDVREQAAQYQEIAQALGGRPLTIRTLDIGGDKPIPYLPLP
                     REDNPALGLRGIRTSLWQPELLRQQLQAVLQVRAAGRCRILLPMITDPAEIRAVRTML
                     LELTGSSGPSVPIAVGAMIETPASAVMADWIAREADFLSIGTNDLTQYTLAIDRGQAD
                     LAARLDGLHPAVLRLIAKTTEAARKHARPVAVCGGLASDPAAVPLLIGLGIHELSMVA
                     GVIPSMKARIARLRSDECASLAARALEQETPQAVRALMQEWAP"
     gene            6999..8762
                     /gene="nagE"
                     /locus_tag="VFB37_17120"
     CDS             6999..8762
                     /gene="nagE"
                     /locus_tag="VFB37_17120"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR01998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HZO24216.1"
                     /translation="MKQILPALQRIGGALMLPIAVLPIAGLLLRLGQPDLLNFPSMAA
                     AGDAIFANLGLLFAIGVAVGLARENHGAAGLASVVGYLVTTKGAEVLIAVPPEALAGM
                     AGTARELAAAAYKAHELAKLSVPAGILSGLIAGALYNRFSDIRLPSYLAFFGGRRFVP
                     IASGFAGLVLAAIFGWEWQSLENGMDALSRTVLHAGAFGLFAYGVLNRLLIVTGLHHI
                     INNIAWFLLGDYNGVTGDLKRFFAGDPSAGAFMAGFFPVMMFGLPAACLAMYHSARPQ
                     RRRAVAGLLGSIALTSILTGVTEPIEFTFLFLAPALYGVHALLTGLAFVIMNALHVRL
                     GFGFSAGLFDYLLNFSKATRPLLLIPVGLLYAVLYYTLFRLVIVKFDLRTPGREESEV
                     LTGPAEGPPEQAALAYLVALGGAENIVSLAACTTRLRLVVREQTAVDADTLMRLGARG
                     LVRPSSNALQVVVGTNADQLAGEMKRALGSARLRTGDAMANAGPVVSPAPSPVAAAAL
                     PATVLAELLAALGGRHNVRSVEEAASRLRINIVDGAALNRVALMSVGVRGIALPAPHC
                     VHVIIGPAAAQAGASLRQLLR"
     gene            8835..9695
                     /locus_tag="VFB37_17125"
     CDS             8835..9695
                     /locus_tag="VFB37_17125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZO24217.1"
                     /translation="MARAFWKGAISFGLVYIPVEMYSAAQGNTIDLDYLDKRDFSPIG
                     YQRYNKRTGKPVEWKNIVRGYQYQKGRYVALSEEDFRQANVKASQTIEITSFTEASSI
                     AAEYYETPYYLGAQKGGQKVYALLREALKSTGKVGIATVVVRTRQHLAVLSPQDNLLM
                     LNMLRFADELRSPAGLELEAPSAKTAGLKPGELSMAEKLIGEMSAPWKPAQYHDTYRE
                     DLMARIKEKIRNREAHTLTAPERAEKPQKSAEVIDLMEVLKQSLGQGGRRKPPSRAHA
                     KLGRRKSARA"
     gene            complement(9656..11194)
                     /gene="hutH"
                     /locus_tag="VFB37_17130"
     CDS             complement(9656..11194)
                     /gene="hutH"
                     /locus_tag="VFB37_17130"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZO24218.1"
                     /translation="MSRHTPLELGARPLTLAELRRIYREPIQVGIHERALAALREAHE
                     ATNRLAAADAPAYGINTGFGLLAQTRIPSSQRSLLQRNILLSHSAGVGPLLDDSVVRL
                     SMVLKLASLLQGFSGVSEQLARFLEKLINSGLHPCVPAQGSVGASGDLAPLAHLSLVT
                     IGMGALRRHGEIRPAAEALAQEGLSPLELGPKEGLALLNGTQVSTALALAGLFELEEA
                     FAAAVVSGALSVDALQGSDAPFDARIHALRGHPGQAQVAQVYRELLSGSSIRESHRTC
                     ERVQDPYSLRCQPQVMGACLDLITQSAETLLTEANAVTDNPLVFAASGEVLSGGNFHA
                     EPVAFAADILALACAEIGSLAERRIALLVDPKMSGLPPFLAQDSGVNSGFMMAQVTAA
                     ALVAENRMLCHPASIDSIPTSANQEDHVSMATHGARRFLGMTANLLQIIAIEFLSACQ
                     GIDFRRPLQTSSPLQQAHGALRAHVPFATQDRLLAQDIEVAAKVIRTPAVQALADFLL
                     PSFA"
     gene            complement(11191..>12444)
                     /gene="hutI"
                     /locus_tag="VFB37_17135"
     CDS             complement(11191..>12444)
                     /gene="hutI"
                     /locus_tag="VFB37_17135"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZO24219.1"
                     /translation="ASMGSTCPSSGPDAPMSDALTLWRNATLATCDEAMRIIERGALL
                     TRRGVIEWIGPEQALPSAPPPTEVQDLGGAWVTPGLIDCHTHLVFAGTRATEYAERLR
                     GRSYEEIARAGGGILSTMRAVRAASEQQLFDASVPRLRALLGEGVTTIEIKSGYGLTL
                     ADERKMLRVARSLERAFPVTVKTTLLAAHTLPPEYRGRADEYIRVICEEWLPALHAEE
                     LVDAVDVFCETIGFDLAQSERLFAAARGLGLPVKMHAEQLSNLGGSRMAAGHGALSCD
                     HLEFSSAQDAAALAAAGTVAVLLPVAYYCLGGGQRPPIEALRAARTRMAVATDCNPGS
                     APGTSLLLAMSMATRLFGLTAEEALLGVTRNAARALAEEHRGVLAVGKIADFVAWEVR
                     AVEELSYWIGFNPRRRLVRAGRISS"
BASE COUNT         2067 a         4045 c         4199 g         2133 t
ORIGIN      
        1 tggacgcagc ggtcggattt cttcgtgacc ttcgatcgca cctcgaatct ggatgaggcc
       61 gcgctcacct cgctcgatgc ttcgctggct tacaatgtca ccaagtggct ggcgatcacg
      121 gccgaggcgc agaacctgac caattcgaag gtgatcgaat actcggacgt tctctcgcat
      181 ccgcgcgcga tctacgagaa cggccgggtc tactttgccg gggtgaagct gaggttttga
      241 aatgtccaca agcggccggc cacggaggca agcttcgtgg ccggccgcga cggctcgagc
      301 ggctagtcgt atccgagcgc gctcagcacg ctcttcagcc gcaccgcatg cggcctgagc
      361 tgctgctcgt agtgccgatg gcggcccacg gagcggtcat tgagcttctc ggtgacctgg
      421 gcgtagctcg gagtcggcgc gtagcggcga ttctcatgaa agcgcaggca ggcagcctcg
      481 aaaggcaacc cgagatagtc gagcagggcg cgggtgcggc cttcctggtc ggcgacgagc
      541 tcctcgtagc gcagcgtgaa cacctccagc gacaactggc tccgatagtg ggtgacgaga
      601 gcggtcacgg cggccaggtg atgggtgatg tcctcgatcc ggtagccaca attgaagccg
      661 tgcgtcatat tgttggacag catcgagacg cagacatcca ggggatggcg gacgaggtgc
      721 acgatcttgg cctccggaaa ggccatcttc agcagcggca gatagatctc gttgaaaggc
      781 atcttgtcgg tgaagaaggc ggtcgcccgc aacaagccgt actgctcggc gcgcgcgagg
      841 taataatccc gaaacagggt cgcagcgtag tgacagtccg ccatccacgt gtgagccaga
      901 ttttccggga agcgttccga accgggaaag aggctgccgg tcagcttgct gatgtcgtcc
      961 gcaaagggca gctcgccccc ggccagcacg gccgagtggc tggcgatgat ctgttcgacg
     1021 agcgtggtgc ccgagcgggg gaaacccatg acgaagatcg gttggggcgt ccctgtgcgc
     1081 ggcttcgccc gcggcagcag cgccacatgg tcgctcgtaa agaactcttt gcagcgctca
     1141 aagagcgcat cgaccgcctc gcagcgatag tcggatccgg gcgtctcctt gtgcagcttg
     1201 gctttgccgt ggacgaaatc ctcccaggcg gcggcgtaat ggcccagccg atcgtgcagg
     1261 cggccacggg tgagctgggc ctcaccgccc agagctcccg cggcatcgag tatgcccagg
     1321 gcctcggccg gctttccgat tcgcgccagg aagttggccc gcagaaggtt gacctgctcc
     1381 gcggaggaga ccgcttctgc ccgatcgagc agctccgcgg cgcgcgccag atcgcctcgc
     1441 gcctcgtgca gagtggacca gtaggcgagt gtcttcatgt cctgcggggc gagctcgtga
     1501 gattgcccga agtagccttc ggcctcatcc gtacggccct gcctcatcag gttcaaagcc
     1561 agattcatca ggaccgcggc atgcgggccg gccagctcga gcgctttgcg gaagtgccat
     1621 tcgccggaag gcatatcgtt catctgcgag agaatcgtgc cgaacagatt gtgcacatgg
     1681 gcattgcgcg ggttggcgcg caagcctgca cgcgccactt tgtcggcatc ggtgagccgg
     1741 ccgtgcgtca ggaaaagctg cacgagctcg ttgaaggccc agtccgcgcg cggatcgatg
     1801 ccgatcacgg tgtccagcgt gcgtgcggcc tgcccgaact gaccacgccg ctgctggatg
     1861 agggcccgca gctcgagcgc gttgatatcc cgtccggcgg tcttgagccg ctcttcggcc
     1921 gccgtttccg cgacccgcag atcgccgcgc gccagcgctt cgcgcgcgcg gaggacgagg
     1981 ggatggggcg tggtgatcat tcgcgaaacc tcgagcggcc ttgccggggt gcaaaaagcg
     2041 ggagcgctat ggtgcccgct tcagagctgt ttgtttaggg ctgcgcacgt gccagcaaga
     2101 tcgaagaccc tcacactgat tgcgctcctg gtatgcgcct gtattgcagg ggtgccgacg
     2161 agcgccgccc ccagcatgag cgccgccccc agcatgagcg ctgctcccag cgtgggcgct
     2221 gcttccagcg tggatgtcat ccctctcccg gctcacgttc ggcccggcat cggacggttc
     2281 acgatcgcct ccggcacgca gctctcggtg tccggcgatg ctcgagccct gcagatcgct
     2341 gagtattttg cgcagcttgt gtccaagacg cgtgcgacgg ggattgaggt ggtgcgggca
     2401 acagccgggc gcgaccccgc cgggacgatc ctcttcgaaa tccggccgca gagcgcgact
     2461 gcagacccgg agagctactc cctggatatc gccccggagc ggatcgtgct cgccgctgcc
     2521 gatgcgcgcg gactgttgta cggggccgtg acgctctggc agctttgcac ggcccgcgcc
     2581 gcgcggtccg atccgatcca gatccccgcg atgagcatcg aggatgcgcc gcgctttcgc
     2641 tggcgcggcc tgttgctcga ttcggcgcgg cattttcagt cgcccgattt catcctgaag
     2701 ctgatcgact ggatggccct gcacaagctc aacgtgctgc agtggcatct gactgacgac
     2761 caggggtggc gcctgcagat cagcagatac ccgcttctga ccagcgtggg cgcctggcgc
     2821 gttccagccg gcgcgggcgc ggctggcgac atcgacccgg gaaccggccg gccgcgtctc
     2881 tacggcggct tttatacaca gcaaaccgtg cgcaagatcg ttgcgcatgc cgcacttcgc
     2941 aatgtcacca tcgtcccaga gatcgacatg ccggggcatg ccacggcggc aatcgttgcg
     3001 taccccacgc tggccagcac acctgcacca ccgaagacgg tgccctccga ctggggcgtc
     3061 tatccaaacc tgttcaatgc cgatgagcag accttctcct ttctggagaa tgtcctgtcc
     3121 gaggtcctcg agctgtttcc cggtcagtat gtcgatgtgg gaggtgacga ggccatcaag
     3181 gaccagtggc acacctcggc gcacatgcag gcgcgcatgc acgagctggg agtgacgaac
     3241 gaacaagccc tgcagagcta tttcatgcgg cggatcgagc aatggctgca tgcgcatggc
     3301 cggcggctcg tcggctggga cgagattctc gagggcggcc tgccgccgcg agcgacggtc
     3361 atgtcctggc gcggcctcga cggagcggtt gccgccgcac gcttgggtca tgacgtggtc
     3421 ctgtcgcctg cgcccgtcct ttatctggat aatcggcaag gaagcggggc aggagagccg
     3481 cctggccggg gacaggtcgt gaccctcgag cagctttatc gcttcgatcc gcgtcccggg
     3541 gcgctgtcct cggaggaagc ctcacacctc ctcggagtgc aggccaacat ctggaccgag
     3601 cacatccgca cggaagagcg cgtgcagtac atggcttttc cccgtgcggc cgccttgtcc
     3661 gagctcggct ggtcgcgccc gcaggatctg agctgggaag gatttgcggc gcgcctgccc
     3721 gatgagctcg ggaggtatcg agcactcggc atacatgcat ccgaggacgc tttcaccgtg
     3781 agcatcgagg cgcggatggc cccagggggc aggggtgtac aggtccaact cgccaagcag
     3841 gccgcgcccg gcgagattcg gtacacgcgc gatcaaagca atccgacgca aacagcgacc
     3901 ctctacacag gtccgctcac cctgccggtc ggaggcgagc tgcgcgcggc ggccttcgat
     3961 gctggcggcg cgctcacggc agtgaccgcg cgctcattgg atgcaggctc catcgcgcgg
     4021 cgctcgagcc aggagctcaa aacctgcacc agccggctcg tgttgaatct cgaggatgat
     4081 gcacccgtcg agggcccgcg ggcgatcttc ctggtagaca tcctgaaccc gtgttggatc
     4141 tacgaggcag tcgacctgtc gcacgtgacg gccgtgaagg cggcggttgg gcaggtgccc
     4201 ttcaactttc agctcgggtc ggcgcgtgat tcgatcggct tcgacaaacc ggcgagtgcc
     4261 gacggagagc tcgaagtgag ggtcgatggt tgtgagggcg agcccatagc ggttttgcct
     4321 ctaacgccgg cccgcgcgag ccccgtggtc accacgttgc ccgcggctcg gatcgccccg
     4381 cgatcagggg tgcatgacct gtgcttccgc tttgcgcagc aggggctgga gccgctctgg
     4441 gtgctcgatt gggtcgagcc tgtcggatag atgccgatgg atgcgacatc ctcgcggctc
     4501 acgctggttt ccccgctgca gggctggtgc gctgcgctgg aggaggcgcc tgaccccgtc
     4561 ttcgctggcc ggatgctcgg cgatggcgtc gcgatcgatc cgaccgggaa ctcgctgtat
     4621 gcgccctgtg ccggcacgct gatcacggtt ccggcctcga agcacgcgct cactctgcaa
     4681 gccgagaacg gcatccaggt cctgctgcac atcgggatcg ataccgtggc cctggccggt
     4741 gaagggttcg aattgcacgt gcgcgcgggg cagccggtga gggccggtga gcggttgatc
     4801 actttcgatc tcgatctgct cgcgcgcaag gcccgaagcc tgctcacgcc tgtgatcctg
     4861 acagggggca ctgacatcgt tgtcagccgc cgttgtgtgg gccgccgggt cgaggtgggt
     4921 gagttcctga tggaagctac gggggcgcct gctgcggtca tcacggccgg ttcggcaagc
     4981 tccgcgttgg aagattccgt ggtcgtgcag tcggagcacg gcattcatgc gaggccggcc
     5041 gcactgcttg cggaggcaat ccggaaactg tctgctgagg tgagcatcgc cgttcgcggc
     5101 cgaacggcga acgcgcgcag cccgatcgcg ctgatgtccc tgggaattcg ccatggggag
     5161 cggattgcga tccgcgcatc gggcgcggat tcggccgccg ccctggcggc ggtgaagcgc
     5221 acgttggaag cgcgactccc tgaggctgct cgctcgacga tcagcgccgc cggcagtgac
     5281 ccgccacaga aagctcctgc tgaaggcgcc gctcccgagc agcgggctcg tgaagttacc
     5341 ggcgtgatcg caagcccggg agtcgccgtc ggatatgcgt atcagctggc gcgtatcgat
     5401 ctggaggttg ccgagacagc accggacgca gcccgggagc ggttcgcgct cgagaacgcg
     5461 cgcgcggcag tgcgcacaga gttgcaacgc accctcgatc gcgagcaggg tgcgccgcgc
     5521 acgatcgccg aagcgcatct cgggttgctc gaggaccccg agctgctcga gcgcgcggca
     5581 aagctgatca gcctcggcaa gagtgcaggc ttcgcctggc gagccgcgct gcgcgaagcc
     5641 gcggaaggtc tgcgaggtct cgaggattcg cgcctgcgcg aacgcgccga cgatctcctc
     5701 gatctcgagt cgcgagtcct gtcgatgctg agtggatctg ccgccgagag ccccgagctc
     5761 cctgccgatt ccatcctcat gggggatgag ctgctgccct ctcaactggt cgcgctggat
     5821 gcgcatcggc tcgccggtat ctgcgtggcc cgaggtggac ccacttcgca cgtatcgttg
     5881 atggccatgg ccatgggggt accgatgctc gtggccgcgg ggcacaggat tttgcaggtc
     5941 gcgacgggga ctgcagttct gctggatgca gagtccggcc gcctgcacat tgggctcgcg
     6001 gagcaggaga tcgaggccgc gcgccggcgg attgccgggc agcgtgcacg gcatgcagcc
     6061 gcgttggcgg ccgcccaggc cgaatcccac acggccgatg gggtgcggat cgaagtattc
     6121 gccaacattg gatcgacgct cgatgcgcag ctcgcgctgc aaaacggtgc cgaaggttgt
     6181 ggcctgctgc gaacggagtt tctttttctg gagcggcagt ccgcgccgga cgtgcgtgag
     6241 caggccgcgc agtatcagga gatcgcgcaa gcccttggcg ggcggccact taccatccgc
     6301 accctcgaca tcggcgggga caaaccgatt ccctacctgc cgctgccgcg tgaggacaat
     6361 cccgccctcg ggttgcgcgg catccgcacg agcctgtggc agcccgagct gctgcgccag
     6421 cagctgcagg ccgtacttca ggtccgggcc gccggccgtt gtcgcattct gctgccgatg
     6481 atcacggatc ccgccgagat ccgtgcagtg cgcacgatgc tgctcgagct gacgggctcc
     6541 agcggcccga gtgtgccgat tgcagtcggt gcgatgatcg agacgcccgc atcggccgtg
     6601 atggccgact ggatcgcgcg cgaggcggat ttcctgtcca tcggcaccaa cgatctgacc
     6661 cagtacacct tggcgatcga ccgcggacag gcggatcttg ccgcccggct cgatggactg
     6721 catccggccg tcctgcgcct gatcgcgaaa acaaccgaag ccgcgcgcaa gcacgcccgt
     6781 cccgtggcag tctgcggtgg cctggcctcc gaccccgcag cggtcccgct cctcatcgga
     6841 ctgggcatcc acgagctctc gatggtggcc ggggtgatcc cttcgatgaa ggcacggatc
     6901 gcgagacttc gcagcgacga gtgtgcctcg ctcgcagcgc gggccctgga acaggaaacc
     6961 ccgcaggccg tgcgggcact catgcaggag tgggcgccat gaagcagatc ctgcccgcgc
     7021 tgcagcgcat tggcggcgct ctcatgctgc cgatcgccgt gttgccgatc gcgggcctgc
     7081 tgctgcgcct gggtcagccg gacctgctga actttccctc catggccgcg gcgggcgatg
     7141 ccatcttcgc gaacctcgga ttgctgtttg ccatcggcgt tgcggtcggg cttgcgcgtg
     7201 agaatcacgg ggcggccggt ctggccagcg tggtgggcta cctcgtgacc accaaggggg
     7261 ccgaggtgct gatcgccgta ccgcccgagg cgctcgccgg gatggccgga acggctcgcg
     7321 agcttgcggc cgcggcgtac aaggcgcacg agctggccaa gttgagtgtg ccggcgggaa
     7381 ttctctccgg cctcatcgct ggcgcactct acaaccgctt cagcgacatc cggctgccga
     7441 gttatctcgc cttcttcggc gggcgccgct tcgtgccgat cgccagcggc tttgccgggc
     7501 tggtgctcgc tgccattttc ggctgggagt ggcaaagcct cgagaacggc atggatgccc
     7561 tgagccggac cgtcctgcac gccggggcat tcgggctgtt tgcctatggc gtgctgaatc
     7621 gtctgctgat cgtgaccggg ttgcaccaca tcatcaacaa catcgcctgg ttcctgctgg
     7681 gcgattacaa cggcgtcacg ggcgatctca agcggttctt cgccggagac cccagcgcag
     7741 gcgccttcat ggccggcttc ttcccggtga tgatgttcgg cctgcctgcc gcttgcctgg
     7801 ccatgtacca tagcgcgcga ccccagcggc ggcgcgcggt cgcaggactg ctgggctcga
     7861 tcgcgttgac ctcgatcctg accggcgtga ccgaacccat cgagttcacc ttcctgttcc
     7921 tcgcgccggc cctctacggc gtgcacgctc tcttgacggg gctcgccttc gtcatcatga
     7981 acgccttgca cgtgcggctc ggattcggct tctcggccgg attgttcgat tacctgctca
     8041 acttcagcaa ggccacgcgt ccgctgctgc tgatcccggt cggtctgctc tacgcggtcc
     8101 tgtactacac cctgttccgg ctcgtcatcg tgaagtttga tcttcggact cccggccgcg
     8161 aagagagcga ggtgctcact ggccccgcgg aggggccccc tgagcaggcg gcactcgcct
     8221 atctcgtggc actcggcggc gccgaaaaca tcgtctcgct ggctgcatgc acgacgcgcc
     8281 tgcgcctggt cgtgagagag cagacggcgg tggatgcgga tacgctgatg cgattgggcg
     8341 cgcgcggcct cgtgcgaccc tcgtccaatg ctcttcaggt cgtcgtcggc acgaatgccg
     8401 atcagcttgc cggagagatg aagagagcgc tcggctctgc gcgtctgcga accggagatg
     8461 cgatggcaaa cgccggtccc gtggtcagcc ccgcgccgtc gccagtagcg gccgctgccc
     8521 tgcctgcgac cgtgctggcg gagctccttg cagcgctggg cggtcgccac aacgtgcgca
     8581 gcgtcgaaga agctgccagc cgcctgcgta tcaacatcgt ggacggcgcc gcgctgaatc
     8641 gggttgcgct gatgtctgtg ggggtgcgcg gcatcgccct tccagctccg cactgcgtgc
     8701 atgtcatcat cgggccggcc gctgcccagg cgggcgcctc gctacgccag ctgctgcgct
     8761 gaggaatgga acttgcccgc cggatgcggc gttcattcct gcgtcacgga accgagctca
     8821 cgagggtcac ccgtatggcc agagcattct ggaaaggcgc tatctcattc ggactggtct
     8881 acatcccggt ggagatgtac tccgctgcgc agggcaacac gatcgacctg gattatctgg
     8941 acaagcgcga tttctcgccc atcggctacc agcgctataa caagcgcacc ggcaagccgg
     9001 tggagtggaa gaacatcgtg cgcggctacc agtatcagaa aggacgctac gtcgcgctct
     9061 cggaggagga tttccgccag gcgaacgtca aggcctccca gacgatcgag atcacgagct
     9121 tcacggaagc gagcagcatc gcggccgagt actacgagac cccctactac ctcggggcac
     9181 agaaaggcgg tcagaaggtt tatgcgctgc tgcgcgaggc gctgaagagc accggcaagg
     9241 ttggcatcgc gaccgtggtg gtgcgcacgc gccagcatct cgctgtcctc tcaccccagg
     9301 acaacctgtt gatgctcaac atgctgcgat tcgcggacga gctgcgctcg cccgccggct
     9361 tagagctcga ggccccgagc gccaaaaccg ccggtctgaa gccgggcgag ctctcgatgg
     9421 ccgagaagct catcggggaa atgagcgcgc cctggaagcc ggcccaatat cacgatacct
     9481 accgcgagga tttgatggcg cgcatcaagg agaagatccg caaccgcgag gcgcacacgc
     9541 tgacggcgcc cgagcgcgcg gagaagccgc agaagagtgc ggaggtgatc gatctgatgg
     9601 aggtactgaa gcagagtctg ggacaaggcg gccggcgcaa gccgcccagc cgcgctcacg
     9661 cgaagctcgg caggaggaaa tccgcaagag cctgaaccgc gggggtgcga atgaccttgg
     9721 ccgccacctc gatgtcctgc gccagcagcc ggtcctgagt ggcaaacgga acgtgagccc
     9781 gcagcgcgcc gtgcgcctgc tgcagcggac tcgaggtctg caacggcctg cggaagtcga
     9841 ttccctgaca ggcggacagg aactcgatgg cgatgatctg cagcaggttg gctgtcatcc
     9901 cgaggaagcg acgggcccca tgagtcgcca tgctcacatg gtcctcctgg ttggctgagg
     9961 tcggaatgct gtcgatgctg gccggatgac agagcatgcg gttttcggcc accagggccg
    10021 cggccgtcac ctgcgccatc atgaaaccgg agttgacccc gctgtcctgc gccaggaacg
    10081 ggggcaaccc gctcatcttc ggatcgacca gcagcgcgat ccgccgctcg gcgagcgagc
    10141 cgatctcggc acaggcgagt gcgaggatgt cggcggcgaa ggccaccggc tcggcgtgaa
    10201 agttgcctcc cgaaagcacc tcgccgctcg cggcaaacac cagcggattg tcagtgactg
    10261 cgttggcctc cgtcagcagc gtctcggcgc tctgcgtgat caaatcgagg caggcgccca
    10321 tcacctgggg ctgacagcgc agcgaatacg gatcctggac gcgctcgcag gtgcggtgtg
    10381 actcgcgaat gctgctgccc gagagcagct cgcggtacac ctgtgccacc tgtgcctggc
    10441 ccgggtggcc ccgcaaagcg tgaatgcgcg catcgaaagg agcatcactt ccctgcaggg
    10501 catcgaccga gagcgccccg ctgaccaccg cggcggcgaa tgcttcctcg agctcgaaga
    10561 gcccggcaag agcgagcgcg gtggagacct gggtgccatt caaaagcgca agtccctcct
    10621 tggggccgag ctccagaggc gacaggcctt cctgagcgag cgcttcggct gcgggccgga
    10681 tctcgccgtg gcgtcgcagc gcgcccatgc cgatcgtgac cagcgacaga tgcgcaagcg
    10741 gcgccagatc tccggaagcg cccacggagc cctgcgcagg cacgcacggg tgaagccccg
    10801 agttgatgag cttctccagg aagcgggcca gctgctcact cactcctgag aatccctgca
    10861 gcaggctcgc gagcttcagc accatgctca gccgtacgac cgagtcatcc agcagcggcc
    10921 ccacaccggc gctgtgggaa aggaggatgt tcctttgcag caagctgcgc tgggaagagg
    10981 gaattcgcgt ctgcgccagc aatccgaagc cggtgttgat accgtaggcg ggcgcatcgg
    11041 ccgcggcgag gcggttcgtc gcctcgtgcg cttcgcgcag cgccgccagc gcgcgctcgt
    11101 ggatgccgac ctgaatcggc tcgcggtaga tgcgtctcag ctcggccaga gtgagcggcc
    11161 gggcgccaag ctccagaggc gtgtgtcggc tcatgagctg attctaccgg cccgcacgag
    11221 ccggcgccgc gggttgaaac cgatccagta gctgagctcc tccacggccc ggacctccca
    11281 ggcgacgaag tcggcgatct tgccgactgc caggaccccg cgatgctctt cggccagagc
    11341 tcgtgcggca ttgcgggtta ccccgagcaa tgcttcttct gcagtgagcc cgaacaaccg
    11401 tgtcgccatg ctcatcgcca gcagcaacga ggttcccggc gccgaacccg gattgcagtc
    11461 ggttgcaacg gccatgcggg tgcgcgcggc gcgcagggct tcgatcggtg gccgctggcc
    11521 gcctccgagg cagtaatagg ccaccggcag caacaccgcc acggtgccgg ccgcagcgag
    11581 ggctgccgcg tcttgggcgc tggagaattc caggtgatcg cacgaaaggg cgccatggcc
    11641 tgcggccatg cggctgccgc cgaggttgga gagctgttcg gcatgcatct tgacgggcag
    11701 accgaggccg cgggccgcgg cgaacaatcg ctcggactgc gcaagatcga agccgatcgt
    11761 ctcgcagaac acgtccaccg catcgaccag ttcttccgcg tgcagcgccg ggagccactc
    11821 ctcgcagatc acccggatgt actcatctgc gcgcccgcgg tattcgggtg gcagcgtgtg
    11881 cgccgcgagg agtgtcgtct tcaccgtaac gggaaaagca cgctccagcg agcgcgcgac
    11941 tcgcaacatc ttgcgctcat cggccagcgt gagcccgtac ccggacttga tctcgatcgt
    12001 cgtgacccct tcgccgagga gggcccgcag ccggggcacg ctcgcatcga agagctgctg
    12061 ctcggaggcc gcgcgaacgg cgcgcatcgt cgagagaatt ccaccccctg cgcgagcgat
    12121 ttcctcgtaa gagcggcccc gcaggcgttc ggcgtattcg gtggcgcgtg taccggcaaa
    12181 gaccaggtgg gtgtggcagt cgatcagacc gggtgtcacc cacgccccgc cgaggtcctg
    12241 tacttcggtc ggggggggtg ccgagggcag agcttgctcc ggaccgatcc actcgataac
    12301 gccgcggcgc gtcaacagcg cgccccgctc gatgatgcgc atcgcctcat cgcaggtggc
    12361 gagtgttgca tttcgccaga gtgtcagggc atcgctcatc ggcgcatcag gacccgagga
    12421 agggcaagtt gagcccatgc tcgc
//
LOCUS       DASRHN010000423        16854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1975070, whole genome shotgun sequence.
ACCESSION   DASRHN010000423 DASRHN010000000
VERSION     DASRHN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975070"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(643..882)
                     /locus_tag="VFB37_17140"
     CDS             complement(643..882)
                     /locus_tag="VFB37_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24220.1"
                     /translation="MDIIHASAVEPQSQSRGLTHNVVTFPRRTSGAFWLRPVAAQRLR
                     IRELFDSSLDIATVAGLVRLSPERVFEIIRESRQQ"
     gene            complement(1300..2505)
                     /locus_tag="VFB37_17145"
     CDS             complement(1300..2505)
                     /locus_tag="VFB37_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZO24221.1"
                     /translation="MLTDTDVRQAKASGEPYKLADGGGLYLFIAHSGRKTWRYDFRFL
                     RRRQTLTIGPYPEVSLKEARRRHRLARERLEAGENPATAKQREKAARNTAAANTLAAV
                     ANEWLEEVRPHRSHSWGVLIGGWWANHINPVLGSRPLDAVEPSDVLGLMRKIAAAGHP
                     RTAECVRWAISRVYGYAIRNLKTRVNPAREIQRAIQLPPTRHHPSLTAKELPDFLRKI
                     DGYQGRPETRIALKLLALTFVRGSELVGAAWSEIELDDKQWRIPPERMKMSEQHIVPL
                     SRQAIALLKELKPLSGHFPHLFPHLGDPKRAMSDETIREAFKTLGYGGKFTPHGIRST
                     ASTLLNEQGWRPDVIERQLAHAERDRIRAAYNHADYLDERRKMMQAWANYLDALSAEG
                     KVINIKSRA"
     gene            2831..3382
                     /locus_tag="VFB37_17150"
     CDS             2831..3382
                     /locus_tag="VFB37_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24222.1"
                     /translation="MRISDDRYSRERLCLDLALRFLRHEARTQTIRAWTGLTDDRIRN
                     LYRSYMSHSSRFVPRHRGKSPHQVAYFTRSLRVQWETAQLASLFSLLHVVPAQSGAEV
                     AGSLPGLMRGERLCYAFEIYRATIPSAQISFEHAVFLALALARGDQLRLGKCAGCGAL
                     MVVERFPIREKPCQDCISLATHG"
     gene            3481..4383
                     /gene="nudC"
                     /locus_tag="VFB37_17155"
     CDS             3481..4383
                     /gene="nudC"
                     /locus_tag="VFB37_17155"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HZO24223.1"
                     /translation="MEQGRPNFFAGPYADRRADVREEADWPAAARSDPSTLYIAARDT
                     TQLVYPAPQPHIAFLTREHPAVSQADPTQLVLLGWFKGVRSVLLELLPDQPFEAPAGA
                     SFEELRPLSPLLGADEAGLLAYARALTIWRERQRFCGVCGARTLPERAGHLIRCTNRS
                     TPHEFFPRIDPAIIVLVTDGERALLGRQATWPEGRYSTIAGFVEPGESLEDAVSREVL
                     EETGVPVQRPRYDSSQPWPFPSSLMIGFQAQAALNSPIRIGHELEDARWFTREEIRSE
                     GIPHVPPSHSISYRLISTWLESGQ"
     gene            4393..5175
                     /locus_tag="VFB37_17160"
     CDS             4393..5175
                     /locus_tag="VFB37_17160"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HZO24224.1"
                     /translation="MCLLVLAWEAHPRYRLIVAANRDEYHERPAAPLGKWALPDAILA
                     GRDLRAGGTWLGIDRGRRFGVVTNFRELQRPLPEAPSRGGLVPAYLRGTVGAGAYLAA
                     LQPTAQAYSGFNLLLADERSLWYASNRAPNFARALEPGVYGLSNEFLDTPWPKLQRVR
                     RRFDAWLAAPSPAPATELFEILADPTRAGAGEQLPRTGLSHEWEQVLSSAFVIHPQYG
                     TRCSTVLLLEDSGAFCIAERRFDSSGAVNGETALSVEAGSWP"
     gene            5281..7113
                     /locus_tag="VFB37_17165"
     CDS             5281..7113
                     /locus_tag="VFB37_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZO24225.1"
                     /translation="MAQRNSFTPARAYGRAASVLLCLIACPLARATGTVEIRGVNDAI
                     KANVLVYLSFERYKKTTDLSSDTLERLHERVEREVQAALRPFGYYEPRVHSDLRDLGH
                     GDWHVTIEIDPGPPVLVDRVDVQIHGPGATDPLFTRITQKLPIHPGDRLSHAAYDSLK
                     GELQRTAATYGYLDAKLTRSELAVDPPNHKAAIALEMQTGERYRFGATLIEQSVINDA
                     LVRRYLRYQQNEPFDFTELLRTQFALDDAQYFANLEVLPGEPDRQSHTVPVNIRADPS
                     RRNWYSIGGGYATDTGPRGTLSWENRRINSLGHRFSVIVQAAQVTKYQVQSNYIIPIG
                     DPAVEKLTLGGVVEQRQLADVNTHSEYLQPSITKVGGSWQYIYFVQAMRTTGESAAET
                     RTDQLLIPGIDIASVPKGYLGEPIFQHGLFVEIKGADGLVGSSARFAQLHLQAVRVFN
                     IHPRWHLLLRDEVGATAVSRFGDFPVVERFFAGGDNSVRGFAYNDLSPTEAVCLKDPQ
                     GNNILTPQGVCRTVTGAVKVGGKDVITGTVELIRDLPRNLGVATFFDYGNAFNSFGAP
                     ALEYSVGVGVRVRLPVLTLGVDIAQPISRPGAGPRLHINFSPKL"
     gene            7120..10839
                     /locus_tag="VFB37_17170"
     CDS             7120..10839
                     /locus_tag="VFB37_17170"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZO24226.1"
                     /translation="MRRLLLIVAGTLIFLAVAVPAAGLYYLTFTESGFRFLVNRIPRR
                     VGEVQLELADPSGTIAHGVRLGRLEVDHHLVHLRFDGIQGRVELLPLLLQTIRTRDGS
                     VDRISIQIKRRTRPPSRSAPFFLPRWLVVSAERAHVGSAVLTLPSGTRLEASDIHASG
                     IARHRSIRFFEAGLQSADAHIEATGQLKAADPLQIDANGRITWTQEGQPAWSVATIAK
                     GDLNVLSLTAHTVTPFRSDFTGEALDLTGHWHWLGNALVHDLDVTAWGGSPVLGLLSG
                     QLALKGDQDGFAAQGPVRSSGLKAGLFDTQFEGAYSQHVLSARHMDIVNRDSHAHVMG
                     HGTITVVRNGPALDLQGKWQDFRWPLTGRDVAFRSASGQFTVSGTWPYKVHLAGSAAA
                     QSLPPMPASVEGTLARDRFSFSSAELDLSDGHASLSGAVVWAPVQSWAVSGRATDINP
                     ARFRADLPGKLSFALNAEGRGFGERNDFAVEIRDLSGRLRGVTASGGGKFSRRGAAWQ
                     FDNVRVALGRTHLALDGSVNHVFDVKFGLSAQDLSLVSAESRGELEASGTLRGTLKEP
                     IIAATAHGRSIHHEGLALDDFDLAVDFDPTPQHQSKIAARLHNLVFRDRKLDELTFKL
                     DGTTSNYAVRLEAQALGLSASARASGPLVNTAWQGQLDALTVTGTESLHLELDRPVDL
                     LVSADALRADWLCLVGQPASLCAEAEWSASQWKATFTANELPLATLTAGVTQAVDYGG
                     RINLHAHVFGAGEEPPEGTMRLDLIDAQLAHRLSSGRIDHTKIGSGVIEINASRAAVH
                     ADIGLESGDIGTIKGAMDIQRNAEDWQKMPVKGELHAHTDQLDLVTLYVPEIDRAAGH
                     LDADMQIAGTLGTPFVNGTLKISEGEIDYYQVNLGMRQVAFDARLTDNGVDFSGSTRM
                     GSGIAKAKGRLEWRDSLPYGKVELEGTDLRVVDVPEAHIDAAPKLQFSINGRKIDVAG
                     AVNVPYAKIVPKDLSGAVRSSDDEVIVGEKQADPSKRFEVTSTITLGLGDHVSIDTTG
                     LTGRLTGNITVRSGYEPVTSATGELSIEQGKYTAYARKLDIQRGRLIFTGGPIGNPGI
                     DIRAIKEFPDVTAGVNVRGTLLQPRMSFFSDPSLTQAQIVSLILAGGSLETAQTRQTG
                     AGSELLAQGGAILAQQLGSRVGIEDVSLESDLTNETSLVLGRYLSPRLYVSYGIALTE
                     QLNVLKLRYSLGNRWTVKTEVGQARGADLVYTIER"
     gene            10958..11518
                     /locus_tag="VFB37_17175"
     CDS             10958..11518
                     /locus_tag="VFB37_17175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZO24227.1"
                     /translation="MKTALIVIDVQESFRHRPYWRTEELPSFIANVQSLIDACRERRI
                     PVLQVLHRELPDHPDNPFAASSGLVRTMPELKLAADAVFYKSVHSAMLGMAPDGGTLE
                     AWLRHHGVEEVLITGIRTEQCCETTARHASDLGFNVRYVTDATLTFPMQARTGRTYSP
                     LELRERTELVLDGRFAQIVSTAQALS"
     gene            11586..12566
                     /locus_tag="VFB37_17180"
     CDS             11586..12566
                     /locus_tag="VFB37_17180"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO24228.1"
                     /translation="MFFVVLPKLVLLDLAGPAEAFRLAGGISPDSYRLRFIGPRRNAR
                     AAIGLELTALEPLPTHLPREAILVITGLSGESMDFGDPDIQRVTAWLGSGVCSSALLV
                     CVCAGSVMAGKAGLLAGRECTTHHAHIEELRATATGSRVVDNRIFVEDGMVFTSAGVT
                     AGLDLALHIIGQQLGPRVASEVARELVVYLRRSGTDPALSPWVLHRNHLHPAVHRVQD
                     AVVRRPAASWTAAELSSVACTSPRNLARLFAEHARCSPLDYVQLIRVALARELVTQTA
                     LDLEDIAARAGFSSAQQLRRVWAKWQPGTPSGFRAAHGQSAAPELQTQSL"
     gene            complement(12554..13480)
                     /locus_tag="VFB37_17185"
     CDS             complement(12554..13480)
                     /locus_tag="VFB37_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZO24229.1"
                     /translation="MSPPMYSIAIHGGAGAMPRANLSAEREKHYLASLESALDAGYAV
                     LQRGDSSLDAVTTAVRILEDDPLFNAGRGAALTREGTAELDAAVMEGRQQRAGAVASV
                     RHVRNPIELARRVMEKSRHVLLVGPGAEEFALEEGMSLVPNLYFRTAERQRQLESEQR
                     GQVVSDLVPSTSGTVGAVAFDQHGTVAAATSTGGMTNKRSGRVGDSPIIGAGTFAKNG
                     VCAVSATGHGEYFIRAVAAHHIAAAVEYRGLSLRDAARELLHRILRALGGDGGVIGVD
                     REGHTVMEFSTEGMFRGARNSRGLRQIAIYND"
     gene            13558..>16854
                     /locus_tag="VFB37_17190"
     CDS             13558..>16854
                     /locus_tag="VFB37_17190"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HZO24230.1"
                     /translation="MPQRIRSILVANRGEIAIRVMRAATELGIRTVAIYSQEDRFSLH
                     RTKSDEAYLVGRDKGPIEAYLDIQDILRIAREADVDAIHPGYGFLSENPEFARACAAA
                     GIVFIGPTPETMSALGNKVSARNLAASAGVPVMPATPALPSDLQAALPLAHGIGYPVM
                     LKASWGGGGRGMRVVEDDAQLTEMLPVAQREARAAFGNGEIYLEKLVRRARHVEVQIM
                     GDRHGQLVHLFERDCTVQRRNQKVVERAPAVFLTETQRAQLCEAALSIGRAAHYVNAG
                     TVEFLQDAESGAFYFIEVNPRIQVEHTVTECATGIDLVKAQIRIAAGARIGTPESGVP
                     PQEQIRLSAHALQCRITAEDPENNFIPDYGKITAYRSPAGFGIRLDAGTAYTGAIITR
                     SYDSLLVKVTSWSASAEETIARMHRALWEFRVRGVVTNLRFLDQLITHPHFARGEYTT
                     RFIDSTPELFQWPRKRDRATRILTYIGETIVNGNAETRHRPRPAHFSMPRLPEARLGP
                     APPGTKQKLDELGPERFSKWMLAETRALITDTTMRDAHQSLLATRMRTLDMAAIAPYY
                     ASLLPGLFSVECWGGATFDVAMRFLREDPWQRLEQLRERMPNLLLQMLFRSANAVGYS
                     NYPDNVVRFFVAHAAKRGIDVFRIFDSLNWIENMRVSIDAVLESGKLCEAAICYTGNL
                     SDPHERKYTLDYYLELARELKAAGTHVLGIKDMGGLCRPHAACVLVQALKKEIGLPVH
                     FHTHDTSGIAAASVLAAIDAGADAVDCAIDSMSGLTSQPNMGSIVEALRHGPREPAID
                     STQLRLVSAYWEQARKLYGAFESDIRSGTSEVYVHGMPGGQYTNLREQARSLGIEAAR
                     WPEVAQTYADVNEMFGDLVKVTPTSKVVGDMTLLMLTSGLTREQVLDPDTDVAFPESV
                     VQLFRGDLGQPHGGLPVALQRKVLKGMAPITQRPGAFLPPADFAAERDRIQQKLPRAV
                     TDDDLAAYLMYPRVWMDYSRDRLQYGDVGILPTPVFFYGMEPGQEMSVDIERGKTLIV
                     RHLATSEVHQDGTRTVFFELNGQPRSVRVPDRSQVATRPPQRKIEPGNPRHVGAPMPG
                     TIASVTVIAGR"
BASE COUNT         2905 a         5477 c         5388 g         3084 t
ORIGIN      
        1 gcttgcgcca atagtccagc gtatcagctg gtttcccatc agcgcatcgc tcacggatcc
       61 gcgcatccaa agcccgccga cgcttgagcg accagacctt gacgagcggc aggtccggaa
      121 gcgcctgatg gtatgcggca atgatctccc gatgcagtgc tggcgcctct gccgtccgcc
      181 tccgcggacg agagtctccg aaggagactc cagattcttg agattgagat tgatgcgagc
      241 gctcagcgac tcgctcaccg tgtcgctcag cgactcgctc agcgactcgc tcagtgacag
      301 cgccacccca tcgcttggag ttggttgccg cagctccggt ttgtcggcgc tcgtgcaagc
      361 gaagcgcgcg ggatcggtgt tcttccaagc gcgagtttcg ccgcaaacca tctgagccga
      421 cagggaattt cccctcaacc ctcgcccagc cctttcgcca ctcagcgcgc gtggccccaa
      481 tcagctctcg aagctgcacg ggactctcag gcaggcctcc ttgctcccac tgcgcgtcga
      541 gaagctcgcg gtaaattgca cgcgagatga tcggccatcc tctggttgat gagagaaaat
      601 ctcgcggaaa ccagggcatc atgaacagat ttgccgaagc gctcactgct gccggctctc
      661 ccggatgatc tcgaacactc gttccgggga tagccggaca agcccggcga cggttgcgat
      721 gtccaggctg ctgtcgaaca gctcgcggat ccgcaggcgt tgggccgcaa ccggtcgcag
      781 ccagaatgct cccgaagttc ggcgcgggaa cgtgacaacg ttatgtgtta ggccgcgtga
      841 ctgcgattgc ggctcaactg ccgaggcgtg tatgatgtcc atgctgattg aacgtccatg
      901 ttagtgtgct gatcgagaga gcccgcggag tagtgcccgc gggctttcgc cgttttggag
      961 ccgaaaatca tgcggcgcgc gactcttggc gatttgctcg cggccgtgct tccatccaac
     1021 gaagcacttc gcccatccgc caagcacttg cgcggccggt caacttgagc ctcgccggga
     1081 atcgcccaag ctcttcgagc ctatagatcg tgctcttgct aagtcccgta atctggcgca
     1141 gctgcgccat ccgcaagaag cgctgcgaca tctcgtccga cattgctaac tccgtggacg
     1201 ctgccctgcg tccgctggag tctcaagtta ctaaagattt cgcctctgtg ttgcgcaaca
     1261 cgggcgtttt cgcgcgatat tgtcgcaaag cgaccgctat caagcgcgcg acttgatgtt
     1321 gatgaccttg ccttcggcgc tcagggcgtc gaggtaatta gcccacgcct gcatcatttt
     1381 tcggcgctca tcgaggtagt ccgcgtggtt gtaagccgcg cggatgcggt cgcgctcggc
     1441 atgggcgagt tgccgctcga tcacatccgg gcgccatcct tgctcgttca gtaacgtgct
     1501 ggcggtcgag cgtatgccgt ggggtgtgaa cttcccgccg tagccgagcg tcttgaaggc
     1561 ttcgcggatg gtctcgtccg acatcgcgcg cttaggatcg ccgaggtgcg ggaacaggtg
     1621 cggaaagtgc ccgctgagcg gcttgagctc cttcagcaac gcgatcgcct ggcgcgaaag
     1681 cggcacgatg tgttgctcgc tcatcttcat gcgctctgga ggtatgcgcc attgcttgtc
     1741 atccagctcg atctcgctcc aagccgcgcc gacgagctcg ctgccacgaa cgaacgtgag
     1801 tgccagcaac ttcaatgcga tgcgcgtctc tggccggccc tggtagccgt cgatctttct
     1861 gaggaagtcg ggtagttcct tggcggtcag gctcggatga tggcgggtgg ggggcaactg
     1921 gatcgcgcgc tggatctcgc gcgccgggtt cactcgcgtt ttgaggttgc ggatcgcgta
     1981 gccgtacacg cggctgatcg cccaccgcac acactcagcg gtgcgcggat ggcctgcggc
     2041 ggcgatcttg cgcatgagcc cgagtacatc ggacggctcg acggcatcga gcggtcggct
     2101 gcccagcacc gggttgatgt gattggccca ccagccgccg atcaggacgc cccacgaatg
     2161 cgagcggtgg ggccttactt cctcaagcca ctcgttcgcg actgccgcga gggtgttggc
     2221 agcagcggtg ttgcgtgcag ccttctcgcg ctgtttagcg gtcgctggat tttccccagc
     2281 ctcgaggcgc tctctagcaa gccggtgccg tcgccgtgct tccttcaggc tgacctcggg
     2341 gtacggaccg atcgtgagcg tctgacgtct gcggaggaag cgaaagtcgt atcgccaggt
     2401 cttgcgcccg ctgtgggcga tgaatagata caaaccgccc ccgtcagcca gtttataggg
     2461 ctcgccagaa gctttcgcct ggcggacatc tgtgtcagtg agcatcgaga tcgcctctct
     2521 gcggccgtcg tatggcggta tgcatgacgg tatcttgtca aattgacggt attcggtgcc
     2581 ccggggcgcg agcagtaccg tcagaactac cgtcgattat ggcgggctgc atcgggtctc
     2641 cgtgggacgt cgtgaggcat tccgcaccga gaataccagc aattgtgctt ggtattgaga
     2701 ctctacgcga cttcctgaaa ctccgcttgg tacagggtca aatatgacca aaaattaaag
     2761 attgagtttt gggatttttt tcccaaaact ggaatccgcc tgcgccaccg ccgccccaca
     2821 gggaaatgcc atgcgcatct ccgacgatcg ctacagccgt gagcggctgt gtctggatct
     2881 ggcattgagg ttcctgcgcc acgaggcccg cacccagacg atccgcgcct ggactggcct
     2941 caccgacgac aggatccgca atctgtaccg ctcgtacatg agccactcga gtcgcttcgt
     3001 gccgcgccat cgcggcaaat ctccgcacca ggtggcctat ttcacgcgct cgctgcgcgt
     3061 gcagtgggag accgcgcagc tcgccagcct gttcagcctg ttgcacgtcg tgcctgcgca
     3121 gtcaggcgcc gaggtggcgg gttcgctgcc ggggctgatg cgaggcgaga ggctctgcta
     3181 tgcgttcgag atctaccggg caaccattcc ttccgcgcag atctctttcg agcatgcggt
     3241 gttcctggcg ctggcgcttg cacgaggcga tcagctccga ctcggtaaat gcgccggctg
     3301 cggcgccctc atggtggtcg agcgatttcc gatccgtgag aagccttgcc aggactgcat
     3361 cagcttggcg acgcacggct gagcagccgc tgccccgctc gctgtcactc gccgggacga
     3421 gccacggccg ctcacagctg actgatagaa tgagcgcccg tcggcagttc agcggcgctc
     3481 atggagcagg gtcggcccaa tttcttcgcg ggtccatacg cggacaggcg tgccgatgtg
     3541 cgcgaggaag ccgactggcc ggcggccgcg cggtccgatc ccagcacact ctatatcgca
     3601 gcgcgagaca cgacccagtt ggtatatccg gctccgcagc cacacattgc gttcctgaca
     3661 cgtgagcatc cggccgtgag ccaggccgat ccgacgcagc tcgtgctgct tggatggttc
     3721 aagggtgtgc gctcggtcct gctggagctc ttgcccgatc agcccttcga agcacccgcc
     3781 ggcgcaagct tcgaagagct caggccgctc tcgccgctcc tcggcgcgga cgaggctgga
     3841 ctgctcgcct atgcgcgtgc cctgaccatc tggcgcgagc ggcaacgatt ctgtggagtc
     3901 tgcggcgcac ggacattgcc cgaacgcgcc ggccacctca ttcggtgcac caatcgcagc
     3961 accccgcatg agttcttccc gcgcatcgat ccggcgatca tcgttctggt cacggacggg
     4021 gagcgtgcgc tgctggggcg gcaagcgacc tggcccgaag gacgctattc cacgatcgcc
     4081 ggattcgtcg agcctggcga gagcctggaa gatgcggtgt cccgcgaagt gctcgaggag
     4141 accggtgtgc ccgtgcaacg cccccgatat gactcttcgc agccgtggcc cttcccttcc
     4201 tcgctgatga ttggttttca ggcgcaagcc gcactgaaca gcccgatccg gatcggccac
     4261 gagttggaag acgcccgctg gttcacacgg gaggagatcc gctccgaggg catccctcac
     4321 gtgccgccgt cgcactcgat ctcgtatcga ctcatctcca cctggctcga atccgggcag
     4381 taggctcgat ccatgtgcct gctcgtgctt gcgtgggaag ctcatccccg ttatcgcctg
     4441 atcgtcgcgg ccaaccggga tgagtaccac gagcgtcccg cggcccccct gggcaagtgg
     4501 gcgctcccgg atgccatcct ggccgggcgc gacctgcgag ccgggggcac ctggctgggc
     4561 atcgatcgcg ggcgtcgctt cggggtggtc acgaatttcc gcgagctgca gcggccactg
     4621 ccggaggcgc cctcgcgcgg cggcctggtg cccgcgtacc tgcgcggcac ggtcggcgcg
     4681 ggcgcttatc tggcagcgct ccagccgact gcgcaggcct actccggctt caacctcctg
     4741 ttggccgatg agcgctcgct ctggtacgcc tccaatcgcg ctccgaactt cgcacgagcg
     4801 ctagagccgg gggtatatgg gctctcgaat gagttcctcg ataccccatg gcccaagctg
     4861 cagcgcgtgc gccggcgctt cgatgcctgg ctggcagcgc cgagccccgc accggccacg
     4921 gagttgttcg agatcctggc cgatcccacc cgggccggcg caggcgagca gctgcctcgg
     4981 accgggctgt cccacgagtg ggagcaggtg ctgtcctctg cattcgtgat tcatccgcag
     5041 tacgggaccc gctgctccac ggtgctgctt ctggaagact ccggggcgtt ctgcattgcc
     5101 gagcgccgtt tcgattccag cggggctgtc aacggcgaga ctgcattgag cgttgaggcg
     5161 ggaagttggc cctgagccgg aatgagcgga tgctgcggcg gctgccctgt ggataggccc
     5221 cgggccacgc gcggcaataa actagaattc cacgattttc cgtgacgtcg cacgttctag
     5281 atggcgcagc gaaactcctt cacgccggct cgggcgtacg gccgggccgc ttcggtcttg
     5341 ctgtgcctga tcgcctgccc gctcgcgcgc gccaccggca ccgtggaaat ccgtggcgtg
     5401 aatgatgcaa tcaaggcaaa tgtactcgtc tacctgagtt tcgagcgtta caagaagacg
     5461 acagatctga gcagcgacac cctggaacgg ctgcatgagc gcgttgagcg cgaggttcag
     5521 gcggcgctgc gaccgttcgg ctactacgag cctagagtgc actcggacct gcgcgacctc
     5581 ggacatggcg actggcatgt cacgatcgaa atcgacccgg ggcctcccgt gctcgtcgac
     5641 cgggtggacg tgcagattca tggcccgggt gcaaccgatc cgctgttcac acgcatcacg
     5701 cagaaattgc ccattcatcc gggggatcgc ctcagccacg cggcgtacga cagcctcaag
     5761 ggcgagctgc agcgcacggc ggccacctat ggctacctcg atgcgaagct gacgcgcagc
     5821 gagcttgccg tcgatccccc gaatcacaaa gcagccatcg cgctcgaaat gcagaccgga
     5881 gagcgctatc gcttcggtgc cacactgatc gagcagagcg tcatcaatga tgctctcgtg
     5941 cgccgctacc tgcgctacca gcagaacgag cctttcgatt tcaccgagct gctgcgaacg
     6001 caattcgcac tggatgatgc gcagtatttt gccaatctcg aggtgctgcc cggggagccg
     6061 gaccgccaga gccacacggt accggtgaac atccgggcgg accccagccg gcgcaactgg
     6121 tactcgatcg gcggcggcta cgccaccgat accggtccgc gcgggacgtt gagctgggaa
     6181 aatcgccgca tcaactcact cggacatcgg ttcagcgtga tcgtccaggc tgcgcaggtc
     6241 acgaagtacc aggtgcagag caactacatc atcccgatcg gcgacccggc cgtggagaag
     6301 ctgacgctcg gtggagttgt ggagcaacgt cagctcgccg acgtgaacac ccatagcgag
     6361 tacctgcaac ccagtatcac caaagtgggc ggcagctggc agtacatcta cttcgtccag
     6421 gcgatgcgca ccacgggcga gtctgccgcc gaaacgcgca cggaccagct gctcatcccc
     6481 ggcatcgaca tcgcgtcggt tcccaaggga tatctcggcg agccgatttt tcagcacggg
     6541 ctgttcgtcg agatcaaggg agccgacggc ctcgtgggct cgagtgcccg atttgcgcag
     6601 ctccatctgc aggcggtgcg cgtgttcaat atccatccgc gctggcacct gctgctgcgc
     6661 gatgaggtcg gggcgaccgc ggtctcgcgc ttcggcgact tcccggtcgt ggagcgcttc
     6721 tttgccggcg gcgacaacag cgtgcggggt ttcgcctaca acgatctgtc accgaccgaa
     6781 gcggtgtgcc tgaaagatcc tcaggggaac aacatcctca cgcctcaggg cgtgtgccgg
     6841 acggtgaccg gtgccgtcaa ggttggcggc aaggatgtca tcacgggcac ggtggaactg
     6901 atccgcgatt tgccgcgcaa tctcggtgtc gcgaccttct tcgactatgg aaatgccttc
     6961 aacagcttcg gtgcgccggc attggagtac tctgtgggcg tgggtgttcg cgtgcggttg
     7021 ccggtgttga ctctcggggt ggatattgcg cagccgatct cccggccggg ggccggaccg
     7081 cggctgcata tcaacttctc tcccaagctg tgaagcgccg tgcgccgcct tctcctcatc
     7141 gttgccggga ccctgatctt tctcgccgtc gccgtgccgg cggccggcct gtactacctg
     7201 accttcaccg agagcgggtt tcgctttctc gtgaatcgca ttccccggcg cgtcggcgag
     7261 gtgcagctcg agctcgccga tcccagcggg acgattgcgc acggcgtccg cctcgggcgc
     7321 ctggaagtgg atcatcacct cgtgcatctg cgcttcgatg gcatccaggg gcgcgtggag
     7381 cttctgccgc tgctgcttca gaccatccga acccgggatg gctcggtgga tcgcatttcc
     7441 atccagatca agcgccggac gaggccgccc agccgctccg cgcctttctt tctgccgcgc
     7501 tggctggtcg tgagcgccga gcgcgcgcac gtcggaagcg cggtgctgac tctgccgagc
     7561 ggcactcgcc tggaggccag cgacatccac gcatcgggta tcgcgcggca ccggagcatc
     7621 cgcttctttg aggcagggct gcaaagcgcc gacgcgcaca tcgaggcgac cggacagctc
     7681 aaggccgccg accccctgca gatcgatgcg aacggccgaa tcacctggac tcaggagggt
     7741 cagcccgcct ggtccgtcgc taccatcgcc aagggtgacc tgaatgtcct gtcgctgacg
     7801 gctcatacgg tgacgccgtt ccgatcggac ttcacgggcg aggccctcga tctgaccggc
     7861 cactggcatt ggctcggtaa cgcgctcgtt catgacctgg atgtcactgc ctggggcggc
     7921 agcccggtgc tgggcctgct ttcggggcag ctggccctga agggtgacca ggacggattc
     7981 gccgcacagg gtccggtccg ctcttccgga ttgaaggcgg ggctcttcga tacgcagttc
     8041 gagggagcct actcgcagca tgtcctctcg gcgaggcaca tggacatcgt gaaccgggac
     8101 tcccatgccc acgtgatggg ccatggcacg atcaccgtgg tgagaaacgg tccggcactg
     8161 gacctgcagg ggaagtggca ggacttccgc tggccactga ccggtcggga tgtggcattc
     8221 cgcagcgcgt cgggccagtt taccgtgagc gggacctggc cctacaaggt gcatctggcc
     8281 gggagcgccg cggcgcagag cctgcccccc atgccggcga gtgtcgaggg cactctcgcc
     8341 cgagaccgtt tctcattcag ctcggcggag ctcgacctgt ccgacgggca tgccagcctc
     8401 agcggtgcgg tcgtctgggc gcccgtgcag agctgggccg tctccggacg cgcaaccgac
     8461 atcaaccccg cgcgctttcg agccgatctt ccgggcaagt tgagttttgc tctcaacgcg
     8521 gagggccgcg gattcgggga gcgcaacgac ttcgccgtcg agattcgcga cctcagcggc
     8581 cggcttcggg gtgtcacggc cagcggcggc ggtaaattct cccgccgcgg ggccgcctgg
     8641 cagttcgaca acgtgcgagt ggccctcggg cgcacccatc tcgcgctcga tggcagcgtg
     8701 aatcatgtct tcgacgtcaa gttcggcctc tcggcccagg atctgagcct ggtctcggcg
     8761 gagagccggg gcgagctcga ggcgagcgga acgctgcgcg gcacgctgaa ggaacccatc
     8821 atcgctgcga ccgctcacgg ccgaagcatt catcatgaag gcttggcact cgatgacttc
     8881 gatctcgcgg tggacttcga tccgacccca cagcatcagt cgaagatcgc cgcccggctg
     8941 cacaatctgg ttttccggga ccgcaagctc gacgagctga cgttcaagct ggatggcacg
     9001 acatccaact acgcggtgcg cctcgaggct caggcactcg gcctttccgc cagcgcgcgc
     9061 gccagcgggc ctcttgtcaa cactgcgtgg cagggccagc tggatgcgct cacggtgaca
     9121 ggaactgagt cgctgcacct ggagctcgat cgtccggtgg acctcctcgt ctcggcggat
     9181 gctttgcgag ccgactggtt gtgcctggtg ggccagccgg cgagcctgtg cgctgaagca
     9241 gaatggtcgg cttcgcagtg gaaggcgact ttcaccgcca atgagctgcc gctcgcaacc
     9301 ttgacggccg gcgtgacgca ggcggtggac tatggaggcc ggatcaacct gcatgcgcac
     9361 gtgtttggtg cgggagagga gccgccggag gggacgatgc gtctggatct gatcgatgcg
     9421 cagctggctc accgcctctc cagcggccgg atcgatcaca cgaagatcgg ctcgggagtg
     9481 atcgagatca acgcgagccg agccgcggtg cacgcggaca tcggcctcga gtccggggac
     9541 atcggcacga tcaaaggcgc gatggacatc cagcgtaatg ccgaggactg gcagaagatg
     9601 cccgtcaaag gtgagcttca cgcgcatacc gaccagctgg atctcgtgac gctgtacgtg
     9661 ccggagatcg atcgcgccgc gggccatctc gatgccgata tgcagatcgc cggtacactg
     9721 gggacgccct tcgtcaatgg cacgctgaag atctcggagg gcgagatcga ctactaccag
     9781 gtcaatctgg gcatgcgcca ggtcgcattc gatgcgcgcc tgacggacaa cggcgtcgat
     9841 ttcagcggtt ccacccgcat gggttcggga atcgccaagg ccaagggccg cctcgaatgg
     9901 cgcgactccc tcccttacgg caaggtggag ctcgaaggca ctgacctgcg ggtagtcgac
     9961 gttccggagg cgcatatcga tgccgcgccc aaactgcagt tcagcatcaa tggtcgcaag
    10021 atcgacgtgg ccggagcggt caacgtcccc tacgccaaga tcgtcccgaa ggatctctcc
    10081 ggagccgtcc gctcgtccga tgacgaagtg atcgtcggtg agaagcaggc ggatccatcg
    10141 aagcgcttcg aggtgacgag caccatcacg ctgggactgg gtgatcatgt cagcatcgac
    10201 acgaccggcc tgacgggccg cctcacgggc aacatcactg tgcgcagtgg ctatgagcca
    10261 gtcacgagcg ccacgggcga gctgtccatc gaacagggca agtacaccgc ctatgcgcgc
    10321 aagctcgata tccagcgcgg gcggctgatc ttcaccggcg gcccgatcgg caatcccggc
    10381 atcgatattc gtgcgatcaa ggagtttccc gacgtaaccg cgggcgtcaa cgtccgcggc
    10441 acgctgctgc agccgcgcat gagtttcttc tccgatccat ccttgaccca ggcgcagatc
    10501 gtctccctca tcctcgctgg cggctcgctc gagaccgccc agacgcgcca gaccggtgcc
    10561 ggcagcgagc tgctggcgca gggaggcgcc attcttgccc aacagctcgg ttcccgcgtc
    10621 gggatcgaag acgtcagcct cgagtccgat ctcaccaacg agacttcgct cgttctgggc
    10681 agatacctct cgccgcgact ttacgtgagc tacggcatcg cactcaccga gcagctcaac
    10741 gtgctcaaac tgcgttacag cctcggcaac cgctggaccg tgaagacgga agtgggtcag
    10801 gcccgtgggg ccgatctcgt gtacacgatc gagcgctgag ccgtccttgg cctgcggcca
    10861 ggctattgtc cgtttttgaa tggtgcctgt ccaatagtgc ggtagatcag ctgcgcaagc
    10921 tgggtcatga tgtggccaat tcaagccggg agcacgcatg aaaaccgccc tgatcgtgat
    10981 cgacgtgcag gagtcgttcc gtcaccgacc ctactggcgc acggaggagc tgccgtcctt
    11041 cattgccaac gtgcagtctt tgatcgacgc ctgcagggag cggcgcatcc ctgtgctgca
    11101 ggtgctgcat cgtgagctgc ccgaccatcc tgataatccg ttcgccgcct catcagggct
    11161 cgttcgaacg atgcccgagc tgaaactggc tgcggatgcc gttttctaca aaagcgtcca
    11221 ttcggcgatg ctcggaatgg cgcccgacgg cggcacgttg gaagcgtggc tgaggcatca
    11281 cggggtggag gaagtgctga tcactggcat ccgcaccgag cagtgctgcg agaccaccgc
    11341 aagacacgca tccgatctcg gcttcaacgt ccgatatgtc accgacgcga ccctgacttt
    11401 cccgatgcag gcacgcacag gacgaaccta cagcccgctg gagctccgcg agcgcaccga
    11461 gctcgtgctc gatggccgct tcgcgcagat cgtttcaact gcgcaggcgc tcagctgagc
    11521 ccggggcgca tcgcgatgtc ctcggacgag cgcacggtgc ggcaggagct ctgaccttgc
    11581 gcgacgtgtt cttcgtggtg ctgcccaagc tggttctgct ggacctggcg ggaccggccg
    11641 aagcatttcg gctggccggc ggcatttcgc ccgacagcta tcgcctgcgc ttcatcggcc
    11701 cgaggcgcaa tgctcgcgcg gcaataggct tggagctgac cgcgctcgag ccgctaccga
    11761 cgcacctgcc gcgcgaggcg atcctggtga tcaccgggct cagtggagaa tcaatggatt
    11821 tcggcgaccc cgatatccag cgcgtgaccg cctggctggg ctcgggcgtc tgctcctccg
    11881 ccctgctggt gtgtgtctgt gccggcagcg tgatggccgg caaagccggc ttgctggcgg
    11941 gacgggaatg tacgacacac catgcgcaca tcgaggagct gcgtgcgacg gcgacaggct
    12001 cccgggtagt cgataacaga atcttcgtcg aggatggaat ggtattcacc agcgccggcg
    12061 tgaccgcggg gctcgatctc gctctgcaca tcatcgggca gcaactgggg ccgcgcgtgg
    12121 cgtcagaggt ggcacgcgag ctggtcgtgt atctgcgcag atcggggacc gatcccgcgc
    12181 tgtcgccgtg ggtgttgcat cgaaaccatc tgcatccagc ggtccatcgg gttcaggacg
    12241 ccgtggtgcg gcgaccggcg gcttcctgga ctgccgctga gctgtcttcc gttgcctgca
    12301 cgagcccgcg caatctggcc cggcttttcg cagagcatgc ccgctgctcg ccgctcgact
    12361 acgtacagct catccgggtc gcgctggcgc gcgagctcgt gacgcagacc gcgctcgatc
    12421 tcgaggacat tgcagcgcgt gcaggattca gctcagctca gcaactgcga cgtgtgtggg
    12481 caaaatggca gccgggcacg cccagcggct tcagggccgc ccatgggcaa agcgcggcgc
    12541 ccgaactgca gactcaatcg ttatagatcg cgatctgccg cagcccgcgc gagttgcgcg
    12601 cgccacgaaa cattccctcg gtgctgaatt ccatgaccgt gtgaccttcc ctgtccacgc
    12661 cgatcacgcc gccatcgccg cccagcgcgc gcaggatccg atgcagcagc tcgcgcgcag
    12721 catcccggag cgacagaccg cggtattcga ctgccgcggc aatgtggtgg gccgcgactg
    12781 cgcgaatgaa atactcgcca tgaccggtcg cagagactgc acacacgcca ttcttggcaa
    12841 acgtacccgc gccgatgatg ggtgagtcgc cgacgcggcc gctgcgcttg ttggtcattc
    12901 cgccggtcga ggtggcggca gccaccgtgc cgtgctggtc gaatgccacg gcgccgaccg
    12961 tgcccgaggt actcggcacg agatcggaga cgacctgccc gcgctgctct gactcgagct
    13021 gacgctgccg ctcggccgtg cggaagtaca gattggggac gagcgacatg ccttcctcca
    13081 gcgcgaactc ctcggccccg gggcccacca gcagcacgtg tcgcgacttc tccatcacgc
    13141 gcctggccag ctcgatggga ttcctgacat gtcggaccga ggcgaccgct ccggcgcgct
    13201 gctgccttcc ctccattacc gcagcatcca gctcggccgt cccctcgcga gtgagcgccg
    13261 cgccacgccc cgcattgaac agcggatcat cctccaggat gcgaaccgcg gtggtgaccg
    13321 catcgaggct gctgtcgcca cgctgcagca cggcgtagcc ggcatcgagc gccgattcca
    13381 ggctggcgag gtaatgcttc tcgcgctcgg ccgacagatt cgcgcgcggc atggcgccgg
    13441 cgccgccatg aatcgcgatg gaatacatcg gtggcgacac gtgttccctc caattcgcta
    13501 tagtccgagc ggtcttcgca gctcgcgcaa cccgcgcatc ttagaggcaa accgctcatg
    13561 ccgcagcgga tccgcagcat cctggtcgcc aatcggggcg agatcgcgat ccgtgtcatg
    13621 cgtgccgcca cggagctcgg gatcagaacc gtggcgatct attctcagga ggatcgcttc
    13681 tcgctgcatc ggaccaagtc cgacgaggcc tatctcgtgg gacgcgacaa gggtcccatc
    13741 gaggcctacc tcgacataca ggacattctt cgcatcgccc gggaagcgga tgtcgatgcc
    13801 attcatcccg gttacggttt cctgtcggag aaccccgagt tcgcacgcgc gtgcgcagcc
    13861 gccggaattg tcttcatcgg gccaacgccc gaaaccatga gtgcgcttgg caacaaggta
    13921 tcggcccgca acctcgcagc ttcggcaggt gtgccggtaa tgcccgcgac acctgcactg
    13981 ccgtccgatc tgcaggcggc actgcctctg gctcatggca tcggctaccc cgtgatgctc
    14041 aaggcgagct ggggcggggg cggacgcggc atgcgcgtgg tggaagacga tgcccagctc
    14101 accgagatgc tgccggtcgc acagcgcgaa gcccgggccg ccttcggcaa tggcgagatc
    14161 tacctcgaga agctcgtgcg ccgcgcgcgg cacgtggaag ttcagatcat gggcgaccgc
    14221 catggccagc tcgtgcatct gttcgagcgg gactgcaccg ttcagcggcg caatcagaaa
    14281 gtggtggaac gcgcccccgc ggtgttcctc accgagaccc aacgggcaca gttgtgtgag
    14341 gccgcgctct cgatcggccg ggcagcccac tatgtcaatg ccggaacggt agagttcctt
    14401 caggacgcgg agagcggcgc gttctacttc atcgaggtca atccgcgcat ccaggtcgag
    14461 cacacggtga cggagtgcgc taccggtatc gatctggtga aagcccagat ccgcatcgcg
    14521 gccggtgcgc gtatcggcac gccggagagc ggcgtcccgc cgcaggagca gatccgcctc
    14581 agtgcgcacg cactccagtg ccgcatcacc gccgaggatc cggaaaacaa cttcattccg
    14641 gactacggca agatcaccgc gtatcgcagc cctgcgggct tcggcatccg gctggacgcc
    14701 ggcaccgcct acacgggcgc catcatcacc cgctcatatg attccctgct ggtgaaggtc
    14761 acttcgtggt ctgcgagtgc ggaggaaacg atcgcgcgca tgcaccgcgc cctctgggag
    14821 tttcgggtcc gcggcgtggt gaccaacctg cgcttcctgg atcagctcat cacgcatccg
    14881 cacttcgcgc gcggcgagta caccacgcga ttcatcgaca gtacccccga gctgttccag
    14941 tggccgcgca agcgggatcg cgccacgcgc atcctgacct acatcggcga gacgatcgtc
    15001 aacgggaatg ccgagacccg tcatcggccg cgaccggcgc acttcagcat gccgcgactt
    15061 ccggaggcga ggctcgggcc tgcgccgccc gggacgaagc agaagctcga tgagctcggt
    15121 cccgagaggt tctcgaagtg gatgctcgcc gagactcgcg ccctcatcac cgacaccacg
    15181 atgcgggatg cgcatcagtc gctgctcgcc acccggatga gaaccctcga catggcggca
    15241 attgcgcctt attacgcgag cctcctgccg ggtctgttct ccgtggaatg ctggggtggt
    15301 gcgaccttcg atgtggcgat gcgattcctg cgcgaggatc cgtggcagcg tctcgagcag
    15361 cttcgcgagc ggatgcccaa tctgctgttg cagatgctgt tccgctccgc taacgccgtc
    15421 ggctattcga actacccgga caatgtcgtc cggttcttcg tcgcgcacgc cgcaaagcgc
    15481 ggcatcgatg tgttcaggat attcgactcg ctcaactgga tcgaaaacat gcgcgtctcc
    15541 atcgacgccg tgctggagtc gggcaagctg tgcgaggccg cgatctgcta cacgggtaat
    15601 ctgagcgacc cgcacgagcg aaaatacaca ctcgattatt atcttgagct cgcacgcgag
    15661 ctcaaggccg ccggtactca cgtgctcggc atcaaggaca tgggtgggct gtgccggccg
    15721 cacgccgcgt gcgtgctcgt acaagcgttg aagaaggaaa tcggcttgcc cgtgcatttt
    15781 catacacacg acaccagtgg catcgcagct gcaagcgtgc tggccgcgat cgatgccggg
    15841 gcggatgccg tggactgcgc gatcgattcg atgagcgggc tcacttcgca gccgaacatg
    15901 ggatcgatcg tggaagcttt gcgccatgga cctcgggaac cggcgattga ttcgacgcag
    15961 ctgcgcctcg tgagtgcgta ttgggagcag gcacgcaagc tctatggcgc gttcgaaagc
    16021 gacatccgct cgggcacatc cgaggtgtac gtgcatggca tgcccggcgg ccagtacacc
    16081 aacctgcgcg agcaggcgag gtcgctcggg atcgaggcgg cacgctggcc ggaagtcgcc
    16141 cagacttacg cagacgtcaa cgagatgttc ggcgatctgg tgaaggtcac gcccacttcc
    16201 aaggtggtcg gagacatgac tctgctgatg ctcaccagcg ggctgacacg ggagcaggtg
    16261 ctcgatcctg acaccgacgt ggccttcccc gagtccgtcg tgcagttgtt tcgcggtgac
    16321 ctcggccagc cgcacggcgg gttgccggtc gcgttgcaac gaaaggttct caaagggatg
    16381 gcgccgatta cgcagcggcc aggggcgttt ctgccgccgg cggacttcgc cgccgaacgg
    16441 gaccgcatcc agcagaaact gccgcgtgcc gtcacggacg atgatctcgc cgcctacctc
    16501 atgtatccgc gtgtctggat ggattactcg cgcgatcgcc tgcaatacgg cgatgtcggc
    16561 attctgccca cgcccgtctt cttctacgga atggagcccg gacaggagat gagtgtggac
    16621 atcgagcggg gcaagacgct cattgtgcgg catctggcca cgagcgaggt gcaccaggat
    16681 ggcacacgca cggtgttctt cgagttgaat ggtcagccgc gctcggtgcg cgttccggac
    16741 cgcagtcagg tcgcgacacg accgccccag cgcaagatcg agcccggcaa cccccgacac
    16801 gtcggcgccc cgatgccggg tacgatcgct agcgtgacag tgatcgcggg ccgg
//
LOCUS       DASRHN010000424         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_650332, whole genome shotgun sequence.
ACCESSION   DASRHN010000424 DASRHN010000000
VERSION     DASRHN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650332"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFB37_17195"
     CDS             <1..364
                     /locus_tag="VFB37_17195"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="E3 component of pyruvate and 2-oxoglutarate
                     dehydrogenase complex; catalyzes the oxidation of
                     dihydrolipoamide to lipoamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZO24231.1"
                     /translation="YTDPEIAWVGLTETQAQTQNIQFRKGAFPWVANGRSLSLGREEG
                     FTKLLFDPDSHRLLGGGIVGTNAGDLISEVALAIETGADAADIGLTIHPHPTLSETVA
                     GAAEAVEGTLTDLYIPKK"
     gene            complement(384..1085)
                     /gene="nth"
                     /locus_tag="VFB37_17200"
     CDS             complement(384..1085)
                     /gene="nth"
                     /locus_tag="VFB37_17200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZO24232.1"
                     /translation="MTPARRRAIFRRLQAANPTPTTELEHASGFELLVAVMLSAHTTD
                     KSVNSATRKLFAVANTPAAIVKLGINGVKPYIRSVGLYNTKAKNLVGLARQILERHAG
                     EVPAERESLEALPGVGRKTASIILNMVFGQSEVGVDTHIFRVANRTGLAPGNTPREVE
                     DALVATTPEQYLKDAHHWLLLHGRYVCTARLPACPKCIIKDLCEYPDKTPPSAIKRSD
                     APVAHRQRKRAPRLM"
     gene            complement(1082..1666)
                     /locus_tag="VFB37_17205"
     CDS             complement(1082..1666)
                     /locus_tag="VFB37_17205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HZO24233.1"
                     /translation="MSISDDIDELLPQTQCTRCGYSGCRPYAEAIANGSAQINQCPPG
                     GSATIAALATLLQRRALPLDPAHGLEGPVLVAVIDEEACIGCARCLPPCPVDAIVGSR
                     RLMHSVLAEWCTGCELCVAPCPVDCIAMVPRTTGRQPEPVLNRQRYAAHLSRHEQFAA
                     RQAALLAQRKQSAGVQPSSSAQPQRWAQAATEDS"
     gene            complement(1672..>2792)
                     /gene="metG"
                     /locus_tag="VFB37_17210"
     CDS             complement(1672..>2792)
                     /gene="metG"
                     /locus_tag="VFB37_17210"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HZO24234.1"
                     /translation="FAGPPYKVPDRVYAHGFITVSGEKMSKSRGTGISPLRYLEIGMN
                     PEWLRYYLAAKLSANVEDLDFNPDDFLARVNSDLIGKYVNIASRAASFLTRYFGGELS
                     YAGDSAVLEREAQGLALAVGQHYEAREFGRAMREIMALADRVNRDFDAHQPWLLAKDP
                     QQHEELQDVCSRALQSFKVLSVILAPVLPRLSARVARELFGLERPFQWDDAAVLPRRI
                     NPYSHLMTRVEAKQLDALFDLTKETSPVIPPAPTAAKAAAPAASVPASVSIDEFRKID
                     LRVARIVNAENVAGADKLLKLTLDVGSDTRTVFAGIKGAYEPTALNGRLTVVVANLAP
                     RKMKFGVSEGMVLAASGEGPGLYLLSPDSGATPGMKIT"
BASE COUNT          496 a          870 c          918 g          508 t
ORIGIN      
        1 ttatacggac cctgagatcg cctgggtagg cctcacggag actcaggctc agacccagaa
       61 catccagttc cggaaggggg ctttcccctg ggttgccaac gggcgctcac tatcgctcgg
      121 tcgcgaggag ggattcacca agctgctgtt cgacccggac tcgcaccggt tgctgggcgg
      181 cggtatcgtc ggcaccaacg ccggcgatct gatcagcgaa gtggcgttag ccatcgagac
      241 aggcgccgac gcagcggata tcgggctcac gatccacccg catcccacat tgtccgagac
      301 ggtggcgggc gccgcagagg ctgtcgaggg cacgctcacg gatctgtaca tcccgaagaa
      361 gtaacagcgc gctgcgctcc caatcacatt agccgcggtg cacgcttgcg ctgacggtga
      421 gccaccggag cgtcagatct cttgatggcg ctcggcggag tcttgtcggg gtactcgcaa
      481 aggtctttga tgatgcactt cgggcatgca ggcaggcgag cggtgcagac gtatcgccca
      541 tggagcagca gccagtggtg cgcatccttg aggtactgct cgggagtagt ggcgacgagt
      601 gcgtcctcga cctcgcgggg agtattcccg ggagcgaggc ccgtgcgatt tgccacccgg
      661 aagatgtgag tatcgacgcc cacctcactc tgcccgaata ccatgttgag aataatgctg
      721 gcggtcttgc gtccaacgcc ggggagcgct tccagagact cccgctcagc gggcacctct
      781 cccgcgtggc gctcgagaat ctgccgggcc aaaccaacca ggttcttcgc tttcgtgttg
      841 tagagtccga cgctcctgat gtacggcttc accccgttga tacccagctt cacgatggct
      901 gccggcgtat ttgccacggc gaagagcttg cgcgttgcgc tgttgacgct cttatccgtc
      961 gtatgcgccg agagcatgac cgccacgaga agctcgaacc cgctcgcgtg ctcgagctcg
     1021 gtggttggag tgggattggc ggcctgaagg cgacggaaaa tggccctgcg cctggctggc
     1081 gtcacgagtc ctccgttgcg gcctgcgccc agcgctgcgg ctgggcactc gaggagggct
     1141 ggacaccggc tgactgcttg cgttgcgcca gcagagcggc ctggcgtgcc gcaaactgct
     1201 cgtgcctcga gagatgtgct gcatagcgct gtcggttgag aaccggttcg ggctgccttc
     1261 ccgttgtacg cggcaccatg gctatgcagt cgacaggaca aggggcgacg cacagctcac
     1321 atccggtaca ccattcagcc agcacgctat gcatcaggcg gcgtgaaccg acaatggcgt
     1381 ccacggggca gggtggcaga catcgtgcgc agccgatgca ggcctcctca tcgatcacgg
     1441 cgacgagtac cggcccttcg aggccgtggg ctggatcgag cggcagagcc cggcgctgca
     1501 gaagagtggc cagtgcggca atggtcgcgg agccgccggg agggcactgg ttgatctgcg
     1561 cagagccgtt tgcaatcgct tccgcgtacg gtcggcagcc gctgtacccg caacgggtac
     1621 attgagtctg gggcaacagc tcatcgatgt cgtcgctgat gctcatccgc gtcaggtgat
     1681 cttcatcccc ggtgtggcgc cggaatccgg tgagagcaga tacaaacccg gaccctcgcc
     1741 gcttgcggcc agcaccatgc cttccgagac gccgaacttc atcttgcggg gcgcgagatt
     1801 cgccaccacc accgtcagcc gcccgttcag cgctgtgggc tcataagcgc ctttgatccc
     1861 tgcgaacacg gtgcgtgtgt cggagcctac atccagtgtc agcttcagca gcttgtccgc
     1921 tccggccacg ttctcggcat tcacgatccg cgccacgcgc aggtcgatct ttctgaactc
     1981 atcgatggaa acgctggcag gaacactggc tgcaggggcg gcggccttgg cggcagtcgg
     2041 ggcaggaggg atcacgggtg aggtttcctt cgtcagatcg aagagcgcat cgagttgttt
     2101 ggcctcgaca cgggtcatga ggtgagagta cggattgatg cgccggggga gcacggcggc
     2161 atcgtcccac tgaaacggcc gctcgagccc gaaaagctcg cgcgcgaccc gggccgacaa
     2221 ccgtggcagc acgggcgcaa gaatcacgct cagcaccttg aacgactgca gggcacgcga
     2281 gcagacgtcc tgcagctcct cgtgctgctg cggatccttg gcgagcagcc agggctggtg
     2341 cgcgtcgaaa tcacgattga cccgatcggc gagtgccatg atctcgcgca tcgcccggcc
     2401 aaactcgcgt gcttcgtagt gttgccccac cgcgagtgcg aggccctgcg cttcccgttc
     2461 cagtacggcg gagtcgccgg cgtagctcag ctcgccgcca aagtaccggg tgaggaagct
     2521 cgccgcccgg ctggcgatgt tgacgtactt tccgatgaga tcgctgttga cgcgggccag
     2581 gaagtcgtcg ggattgaagt cgagatcctc gacattggcg ctcaacttcg ccgccaggta
     2641 gtagcgcagc cactcgggat tcatgccgat ctcgagatag cgcagcggac tgatgccggt
     2701 gccgcgcgac ttggacatct tctcgcccga gaccgtgatg aagccgtgag cgtagacccg
     2761 gtcgggcacc ttgtagggcg gacctgcgaa ac
//
LOCUS       DASRHN010000425         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_981653, whole genome shotgun sequence.
ACCESSION   DASRHN010000425 DASRHN010000000
VERSION     DASRHN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981653"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1900
                     /gene="ilvD"
                     /locus_tag="VFB37_17215"
     CDS             191..1900
                     /gene="ilvD"
                     /locus_tag="VFB37_17215"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZO24235.1"
                     /translation="MPSKQQKADPRASSRLVVDGLKQSPSRAMLRAVGFQDADFLKPQ
                     IGIASTWANVTPCNMHIAELAREAARGADAAGGKSVLFNTITVSDGISMGSPGMRYSL
                     VSREVIADSIETVVGAEGFDGFVAVGGCDKNMPGCAMAMARLNRPSIFVYGGTIRPGA
                     HRRDIISVFEAVGARAAGKISEAQLQEVERTAIPGPGSCGGMYTANTMASALEALGLS
                     LPGSSAQEAVSEAKLSDCRGAGEAVVQLVNSGIRPRDILTRKAFENAITVCIALGGST
                     NAVLHLLAIAHDARVRLRLDDFTRIGKRVPVLADVKPSGRYLMSELIAVGGIQPLMKM
                     LLEAGLLHGECLTVTGRSVAENLANTGMYLAEQRIVRPLSDPLKKDSHLVVLYGNVAP
                     EGAVAKITGKEGLSFSGRARVFDGEERATQAILADEVRAGDVVVIRHEGPKGGPGMRE
                     MLSPTSAIMGRGLGEKVALITDGRFSGGSHGFVVGHISPEAAVGGPIALLRNGDRLTI
                     DATRRAIDVALTAAELRRRRKAWKPRKPYATAGVLAKYAYLVGSASTGAVTAPKTARR
                     SIS"
     gene            2224..>2674
                     /locus_tag="VFB37_17220"
     CDS             2224..>2674
                     /locus_tag="VFB37_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24236.1"
                     /translation="MAAYVHDTQFFTRRTIVLFTIVALHVFLAWALATGLARRAIELV
                     APPLQTEIVEEVQKRDEPPPPPPPQLERPPVEVPPPDVNVQVPVDTQSTAIQDVTNKH
                     VEKPPPPPPPKAVNRVAGGPGKGFPNTEDYYPPASRRLNEQGVATVHV"
BASE COUNT          477 a          845 c          906 g          446 t
ORIGIN      
        1 gcctgtgtca acgcgccccc cgccgggccc tgcggtacgc tttgcaggat aaaggtcggc
       61 cccgccaggg ctgactgcct ggagatttgc aggcgcgccg cgccaaccgt cctcggccgc
      121 tgcagcgcgc gctgttcagt cgccgcgcaa atactttaac ctttaagtac cgagaggaga
      181 cttgtaggcg atgccatcga agcagcagaa agcggatccg cgtgcgagct cgcgtctcgt
      241 ggtggatgga ctgaagcagt ctccctcgcg ggcgatgctg cgagcggtgg gcttccagga
      301 tgcggacttt ctcaaaccgc agatcggtat tgcctccacc tgggcgaatg tcacgccctg
      361 caacatgcac attgccgagc ttgcgcggga ggcggcacga ggcgcggatg cggcgggcgg
      421 caagagcgtg ctgttcaaca ccatcaccgt ctccgatggg atctcgatgg gctcacccgg
      481 gatgcgctat tccctggtgt cgagagaggt gattgccgac tccatcgaga cggtcgtggg
      541 tgcggagggg ttcgacggtt tcgtggccgt ggggggctgt gacaagaata tgcccggctg
      601 cgccatggcc atggcacgcc tcaaccgtcc ctcgattttc gtatacggcg gcacgatccg
      661 cccgggcgcc caccggcgcg acatcatctc ggtattcgag gctgtcggtg cgcgcgcggc
      721 gggaaagatt tccgaggcac agctccagga agtggaacgg accgcgatcc cgggacccgg
      781 aagctgcggc ggcatgtata cggccaacac catggcctcg gccctcgagg cgctcgggct
      841 gtcgttgccg ggcagctcgg cgcaggaagc agtcagtgag gcgaagctgt cggactgccg
      901 cggggccggg gaggcggtcg tgcagctcgt gaactccggg attcgaccgc gcgacatcct
      961 gacccgcaag gccttcgaaa acgcgatcac cgtctgcatc gccctgggcg gctccaccaa
     1021 tgcggtgctg catctgctgg ccattgccca cgatgcgcgg gtccggctca ggctcgatga
     1081 cttcacgcgc atcggcaagc gcgtcccggt cctggccgat gtgaagccga gcggccgtta
     1141 tctcatgtcg gagttgattg ccgtcggggg catccagccg ctgatgaaga tgctgctgga
     1201 ggcgggcctg ctgcatggcg agtgtctgac ggtgaccgga cgcagtgtcg cggaaaacct
     1261 tgctaataca ggcatgtatc ttgctgaaca gcgaatcgtg cggccgctct cggaccctct
     1321 gaagaaggac agtcacctcg tggtgctgta cgggaacgtg gcgccggagg gcgcggtcgc
     1381 caagatcacc ggcaaggagg gactgagttt cagcgggcgt gcgcgggtgt tcgacggtga
     1441 ggagcgcgcc actcaggcca ttctcgccga tgaggtccgc gccggggatg tggtcgtcat
     1501 ccggcacgag gggcccaagg gcgggcccgg aatgcgcgag atgctgagcc cgacgagcgc
     1561 catcatgggc cgcgggctgg gagaaaaggt ggcgctcatc actgacggcc gcttttcggg
     1621 cggcagccat ggcttcgtcg ttggacatat ctcgccggag gccgcggtcg gaggtccgat
     1681 cgcgctgctg cgtaacggcg atcggctcac catcgatgca acgcgacgcg ccatcgatgt
     1741 ggcgctcacg gcggcggagc tgcgccgtcg gcgcaaagcc tggaaacccc gtaaacccta
     1801 tgcaaccgcc ggagtactgg ccaaatacgc atacctggtc gggagcgcct cgacgggtgc
     1861 cgtgaccgcg ccgaagactg ctcgccgcag tatttcttga gcttgggcgg agttgtcagt
     1921 gcaccgggca gaggttagag ttcagcaggc atccggcaat gccaatgtcg cgacgggtcg
     1981 ttgtctggca ggcgcccctc gtataggtga aacgagccag ggggattcac aagcaaagcg
     2041 cgcatcagcc cgagtgcccg agcgcgcacg tttatcaaaa acaagtgagc gacccggcct
     2101 gatcgccggg cagcccgagg ccggatccga caccttgcgg gtaaagagaa acccagctac
     2161 tttgcaccgc ttccgtttcg atccgattgg tccgtatcag taacagcgac gtcccatcaa
     2221 gtcatggccg cctacgtaca cgacacacag ttctttacgc gcagaaccat cgttctgttc
     2281 accatcgtcg cgctacatgt cttcctcgcc tgggcgctcg cgacggggct tgcgcgccgg
     2341 gccatcgagc tggtcgcgcc tccgttgcag accgagatcg tcgaggaagt gcagaagcgc
     2401 gacgagccgc cgccgccgcc gccgccacag ctcgagcgcc cacccgtcga ggtgccgccg
     2461 cccgatgtga acgtgcaggt cccggtggat acgcagtcga cggccattca ggacgtgacc
     2521 aacaagcacg tcgagaagcc gccgccacca ccgccaccga aggcagtcaa cagggtggcg
     2581 ggcggcccgg gcaagggctt cccgaatacc gaggactact atcccccggc ctcgcgccgc
     2641 ctcaacgagc agggcgtggc gaccgtgcac gtgt
//
LOCUS       DASRHN010000426         7239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2457501, whole genome shotgun sequence.
ACCESSION   DASRHN010000426 DASRHN010000000
VERSION     DASRHN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457501"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFB37_17225"
     CDS             complement(<1..396)
                     /locus_tag="VFB37_17225"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZO24237.1"
                     /translation="MAQTVGDFLVARLHAWGVRIIFGYPGDGINGVFGALNRAKGKIE
                     FIQVRHEEMAAFMASAYAKFTGDLGVCIATSGPGAAHLITGLYDARLDHMPVLAITGQ
                     QARAALGGHYQQEVDLPSLFKDVAGAFVQQ"
     gene            complement(564..2357)
                     /locus_tag="VFB37_17230"
     CDS             complement(564..2357)
                     /locus_tag="VFB37_17230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO24238.1"
                     /translation="MSEVWTKWQNQIINGTFPLRRFLGASDHSAVFLTEHKARGLADA
                     AIKLVPAHPAHSDAHLARWKTAAALSHAHLLRAFDSGRCQLGGHPFLFLVMEYAEENL
                     SQVLPRRPLTTDEVRGLLQPTLEALAYLHGQGLAHGQVKPPNILVVGDQVKLASDNIR
                     PLGHHSAAKQGKGTPYDPPEARSGTVSAAGDVWGLGVTMTEALTQSLPSWPEGSENVS
                     LPLTLNSKFVEWVRRCLSRDPSTRPTVAGLLARILVRQPDAPAEAAAAASASDKAPGR
                     PAAPMPSPSAAPGIAAPSVAKATTPAEGPRSSVTSPAAAPSPRITDAPTAAANLPRTA
                     GASIPAAAAALPDTPRTAPAPPAAVSPAKVSQPAGRTGPPERPAGPAAHAPASSIPPS
                     AAREPSAQRESMNASPPITPDDSPRRSSYLPRVLAIVVVFAVVWGALRFFARPATNAT
                     RAPATTSQADSQIATAPASPGDDARSASAPTAPPSSETAASSAPAAQPAAGATSPAVL
                     HEEVPNIPLSARSTIRGHIKISVRVTTDRAGNVVDEVLETPGPSHYFAKLVSSAARNW
                     KFAPLQGRDSQQWLLWFELTRDGATAQATPR"
     gene            complement(2632..4083)
                     /locus_tag="VFB37_17235"
     CDS             complement(2632..4083)
                     /locus_tag="VFB37_17235"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZO24239.1"
                     /translation="MRVRPGQMIAVVALAGSLMSAFGAEPSSSIRDLDTTGEAIPSGP
                     KDEAIARALRQISPKQIRHTIETLVSFHTRSTLSSMEHLPKGQGINAAANWIESELKK
                     YSQACQGCLEVKRDTFTQSPAERVPRPTTITNVYAVLRGSDPARAQYMYLVTGHYDSR
                     NSDTLDARGEAPGANDDASGVAVSLECARVLSQLRPAATIVFVAVAGEEQGLDGSRHL
                     AKLAKEQGWQLLGVLNNDIVGGNTTPGDRLQDKSVVRVYSEGIPALATAEQAKQLETL
                     GYAGDSASRELARAIVESAATYEGDLASGDSVTGGELYPLLEFRRDRFLRGGDHTAFN
                     QEGFAGVRFTEWREDFNHQHQNVRTEDGIEYGDLLKFVDFEYVARVAAVNAAALATLA
                     SAPPPPGNVRLLTKQLDNSTSLEWEGAPGNDTDAHYEIVWRALSSPTWQRFIASKSTS
                     AVVEVSKDNVIFGVRSVDSKGHRSIAVAPMPVR"
     gene            4127..5035
                     /locus_tag="VFB37_17240"
     CDS             4127..5035
                     /locus_tag="VFB37_17240"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HZO24240.1"
                     /translation="MGALQGSSAEDIYTLAVNQRSFDPCLREFIGRHERLFVLTGAGC
                     STESGIPDYRDAEGAWKRAQPVNYRAFMADELTRRRYWARSMVGWPRFGRARPNAAHH
                     ALACLERSGRITRLVTQNVDGLHQAAGCKTVIDLHGRLDGVLCVRCELRTRRAALQRQ
                     LLRRNPEWHGLRAALGPDGDADLERLDFSAFDVPACERCGGILKPDVVFFGESVPQDR
                     VRAAMNALEQADAVLIVGSSLMVYSGYRFAQRAFQAGKPVASVNLGRTRADDLIELKV
                     PQACASALAFLLREELPTSAAAAAVD"
     gene            5066..5602
                     /locus_tag="VFB37_17245"
     CDS             5066..5602
                     /locus_tag="VFB37_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24241.1"
                     /translation="MDESPTVAPAPTRKPIALILTLIVALAGCLIAVGYARWLAQQRL
                     SLQGQLVEQERRVLDLREQLTKSEVAVQEKDAALASRDVALAEATQPELPVRVSFRPA
                     WMGQGMVASFRNVGREQLTLMIEFRDPTAHASRDFALVVQPGATADVGHAEGWLVNSG
                     QSVRVSASGFKSITAFAP"
     gene            5705..6010
                     /locus_tag="VFB37_17250"
     CDS             5705..6010
                     /locus_tag="VFB37_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HZO24242.1"
                     /translation="MKLLPVILSSLLLVTATAYGANSQQQKMKDCNAQATSQSLTGDA
                     RKSFMKTCLSSAPAASDSKLNSQQLKMKQCNADAKAKGLTGADRKTFMSSCLKNPAS"
     gene            complement(6624..>7239)
                     /locus_tag="VFB37_17255"
     CDS             complement(6624..>7239)
                     /locus_tag="VFB37_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZO24243.1"
                     /translation="GFVRHTLGEELCDIWPGVASGVDSLATTRPYYRSSYEFLTRADR
                     PLSGLTLDDARLKRLTIGVQMVGADAMNTPPAHALARRGIVQNVRGYMLYGNYQTANP
                     ASAIVDAVAHGDVDVALVWGPFAGYFASRSPVPLRLEQVTPTLDGPQWPMVYDISVGV
                     RRGDPALRDRIQALLEKEKPAIDTILSEYHVPRPQSVRTAARSS"
BASE COUNT         1289 a         2295 c         2353 g         1302 t
ORIGIN      
        1 ctgctgcacg aaagcccctg ccacatcctt gaacaagctc ggcagatcga cctcctgctg
       61 atagtggccg ccgagcgcag cccgcgcctg ctgtccggtg atcgcgagca cgggcatgtg
      121 atcgagccgc gcatcgtaga ggccggtaat gaggtgggcc gcaccggggc ccgaagtggc
      181 gatgcagact cccagatctc cagtgaactt cgcgtaggcg gacgccatga aggccgccat
      241 ctcctcgtgc cgcacctgga tgaactcgat cttgcccttg gcgcgattca gcgcgccgaa
      301 cacaccgttg atgccatctc cggggtaacc gaagatgatc cgaacacccc acgcatgcag
      361 gcgcgccacc agaaaatcgc ccacggtctg tgccattgct catccctcgc cgcaagcgac
      421 ccgcctgcac tcctagtaac ttcgcgtgcg aggcgcagtt cccgcagcgg acgacagaaa
      481 cggggacggc gcgcgggcgg cccaagctgg agctgtacat gcaccggcga gcggcgcgag
      541 agggccatgg tgagggaacc cgactagcgc ggagtggcct gcgctgttgc gccatcccga
      601 gtgagctcga accacaacag ccactgctgg gagtcgcgac cttgaagcgg ggcgaacttc
      661 caattacgcg cagccgagct caccagcttg gcgaagtaat ggctgggacc gggcgtctcg
      721 agcacctcat ccaccacgtt tccagcgcga tccgtcgtca cacgaaccga gattttgatg
      781 tgaccgcgaa tcgtgctgcg cgcgctgagc gggatgttgg gaacttcttc gtgcaggacc
      841 gcgggagaag tcgcgcctgc cgcgggctgc gcagcagggg ctgaggaagc cgcggtctcg
      901 gaagaaggtg gggctgtcgg agcagaggcc gatcgcgcat cgtcgccggg cgatgcagga
      961 gccgtagcaa tctgggaatc ggcctgtgaa gtcgttgcag gcgcgcgtgt cgcattggtt
     1021 gcgggcctcg cgaagaaccg cagcgccccc cataccacgg caaacacgac gacgatcgcc
     1081 agaactcggg gaaggtagga cgatcgacgc ggtgaatcgt ccggcgtgat tggagggctc
     1141 gcgttcatcg actctcgctg cgccgatggt tcgcgcgcag ccgatggggg tattgaggat
     1201 gcgggagcgt gagccgcagg gcctgcaggc ctttccgggg ggcccgtccg gcccgctggc
     1261 tgagatacct ttgcgggaga cacggcggcg ggaggcgccg gcgccgtacg tggagtgtcc
     1321 ggaagcgccg ccgcagctgc ggggatggat gcgcccgcag ttctcggcaa gttggctgca
     1381 gcagttggcg catcggtaat acgcggcgag ggcgctgccg ccggagatgt caccgatgaa
     1441 cgcggtccct cggccggggt cgtcgctttc gcgactgacg gcgcggcgat accgggcgct
     1501 gcactgggcg agggcattgg ggcggctggg cgtccaggcg ccttgtcgga tgctgaagca
     1561 gctgccgcag cctccgccgg cgcatccggt tgccggacca gaatgcgggc cagaagtccg
     1621 gccaccgtgg gtcgagtgga cggatcacgg ctcaagcagc gcctcaccca ctcgacgaac
     1681 ttcgagttca acgtcaaggg tagcgacacg ttctccgatc cttccggcca ggacggcaag
     1741 ctctgcgtga gagcttcggt catcgttacg cccagccccc acacatcacc ggcagcggag
     1801 accgtgcctg agcgggcctc cggcggatcg tacggtgttc ccttgccttg tttcgcggcg
     1861 gagtggtggc ccagcggccg aatgttgtcg cttgcgagct tcacctgatc gccgaccacc
     1921 aggatgttcg gcggcttcac ctgtccgtgt gccagtccct ggccatgcag ataggccagt
     1981 gcctccaggg tcggctgcag cagcccacgc acctcatcgg tcgtcagcgg gcgccgcggc
     2041 aggacctggg agagattctc ttccgcgtac tccatcacca ggaacagaaa ggggtgccca
     2101 ccaagctgac accgacccga gtcgaaggcg cgcagcaggt gagcgtgtga aagcgcggcg
     2161 gcagtcttcc agcgtgcgag gtgcgcatcc gagtgcgcgg gatgagctgg gacgagcttg
     2221 atcgccgcgt ccgcaagccc ccgcgccttg tgctcggtca ggaatactgc actgtgatca
     2281 gaggcgccga ggaatcggcg taacggaaaa gtgccgttga tgatctgatt ctgccacttc
     2341 gtccagacct cgctcaccga tgcctccacg ctgatgagag cgacgcgccc ggaaggcacg
     2401 ttggtcggaa gatatcatca agagcgggcg aggtgtctct gccgcgagca cggcctgcat
     2461 ttcgcgatga gcacttctgc ggctccagag aatcaccgca agcagacgca gggcacctca
     2521 tcgacccgtt acggtgcgaa tttcaggcgt gatcgcaaga tatctatcct gtaatatcaa
     2581 tgtattatag gattaatgta tagtgcaatc tatatgcagt ttctgaccgt tctagcgaac
     2641 gggcatcggt gcaaccgcga tgctgcgatg gcccttcgaa tccaccgatc ggacgccgaa
     2701 gatcacattg tccttcgaga cctccacgac cgcgctcgtg ctcttggagg cgatgaagcg
     2761 ttgccaggtc ggcgagctca gtgcgcgcca cacgatctcg taatgggcat ccgtatcatt
     2821 gcccggtgcg ccctcccact cgaggctggt cgagttgtcc agctgtttgg tgagcagtcg
     2881 tacattgccc ggtggcggcg gtgccgaggc caatgtcgca agggccgcgg cattcacggc
     2941 cgcgacccgg gccacgtatt caaagtccac aaacttcagg aggtcgccgt actcgatgcc
     3001 gtcttccgtg cgcacgttct ggtgctgatg gttgaagtcc tcacgccatt cggtgaagcg
     3061 cactcccgca aagccttcct gattgaatgc cgtgtgatcg ccgccgcgca ggaaacggtc
     3121 gcgtcggaac tccagcagcg gatacaactc accgccggtg accgaatcac ctgaggccag
     3181 gtcgccctcg tacgtggcgg ccgactcgac gatggcgcgc gcaagctcgc gcgaagcaga
     3241 gtcccccgca tacccaaggg tttcgagttg cttggcctgc tcggcggtcg ccagggcagg
     3301 aatgccttcg gagtaaaccc tcacgacgga cttgtcctgc aggcgatccc ccggagtggt
     3361 gtttccgccg acgatgtcgt tgttgagcac gcccagaagt tgccagccct gctccttggc
     3421 gagcttggcg aggtggcgac tgccatcgag tccctgctcc tcgccggcca cggcaacgaa
     3481 cacgatcgtg gcggcgggtc tgagttgcga gagcactctg gcgcattcca agctgacggc
     3541 cacacccgag gcatcatcat tggcgcccgg ggcctcgccc cgagcatcca gcgtgtccga
     3601 gttacgtgag tcatagtgcc cggtcaccag atacatgtac tgcgcccgag cggggtcgga
     3661 cccgcgcaga accgcgtaca cgttggtgat ggtcgtggga cgcggaaccc gctcggccgg
     3721 gctttgagtg aaggtgtcgc gtttcacctc gaggcagccc tggcacgcct gggagtactt
     3781 cttgagctcg gattcgatcc agttcgcggc cgcattgatg ccctgaccct tgggcaggtg
     3841 ctccatgctg gagagtgtgc tgcgagtgtg gaaggacacc agcgtctcga tcgtgtgtcg
     3901 gatctgcttg ggagagatct gccgcaatgc gcgggcgatc gcctcatcct tgggcccgct
     3961 cgggatcgcc tcgccggtcg tgtccagatc gcgtatcgag ctgctcggct cggcaccgaa
     4021 tgcgctcatc agcgatccgg ccagggcgac cacggcgatc atctgaccgg gacgtacacg
     4081 catgcgagac tcctgcacga atggagcgcg caacatgagg ccactcatgg gagcattgca
     4141 agggtcctcg gctgaagata tctatactct cgcggtgaac cagcgttcct tcgatccgtg
     4201 tctgcgtgag ttcatcgggc gccatgagcg gctgttcgtc ctgacaggtg ccggttgcag
     4261 taccgaatcg gggatcccgg attatcgcga tgccgagggc gcgtggaagc gtgctcagcc
     4321 ggtgaactat cgcgcgttca tggcggatga attgacgcga cgccgttact gggcacgcag
     4381 catggtgggc tggcctcgtt tcgggcgagc aagacccaat gcagctcacc acgctctggc
     4441 ctgcctcgaa cgaagcggcc ggatcacgcg gttggtcacg caaaatgttg acggcctgca
     4501 ccaggccgcc ggctgcaaga ccgtgatcga cttgcatggc aggctggatg gggtgctttg
     4561 cgtgcgctgc gagcttcgca cccggcgcgc cgcattgcag cgccaactcc tgaggcgtaa
     4621 tcccgaatgg cacggcctgc gggcagcgct cggaccggac ggcgacgcgg acctcgagcg
     4681 gcttgatttc tccgctttcg atgtaccggc ctgtgagcgc tgcggcggaa ttctcaagcc
     4741 ggacgtggtc ttcttcggcg agagcgtgcc ccaggaccgg gtgagggcag cgatgaatgc
     4801 tctggagcag gcggatgccg tgctcatcgt cggctcctcg ctcatggtgt attccgggta
     4861 ccgcttcgcc cagcgcgcct tccaggcggg aaagcccgtc gccagtgtga acctcggtcg
     4921 cacccgggcc gatgatctca tcgagctcaa agtgccccag gcctgtgcca gcgccctggc
     4981 ttttctgctt cgggaggagc tgcctacctc cgctgcagcg gctgcggtag attgatgctc
     5041 tgcggactgg aagttctttg ccgctatgga cgagtcgccg actgtcgccc ccgcgcccac
     5101 caggaagccc attgcgctca tcctcacgct gatcgtggcg ctcgcaggat gcctcatcgc
     5161 cgtgggctat gcgcgctggc tcgcgcagca gcggctcagc cttcaggggc agctcgtcga
     5221 acaggagcgg cgcgtgctgg atttgcgcga acaacttacc aagtccgagg tcgccgtcca
     5281 ggagaaggac gctgcactgg cctcgcgcga tgttgcgttg gccgaagcaa cgcagcccga
     5341 gctgccggtg cgggtgtcgt ttcggccagc ctggatgggg cagggcatgg tcgcatcctt
     5401 ccgcaatgtg gggcgcgagc agctcaccct gatgatcgag tttcgcgatc cgaccgcgca
     5461 tgcctcgcgc gatttcgcgc tggtggtgca gcccggggcg accgcggacg tcggccacgc
     5521 ggaaggatgg ctggtcaact ccgggcagag cgtccgggtc tcggccagcg gcttcaagag
     5581 catcacagct tttgcgccct gagcggagat ttatgctggg gcggctgagc cgggctcacc
     5641 gttcagcttg cgctacggct gcaggagcat ttccctgcgc gatgcggcaa cgtacactgc
     5701 gcccatgaaa cttctgccag tcatcctctc cagcctgctg ctcgtgaccg ccaccgccta
     5761 cggggctaac tcacaacagc agaaaatgaa ggactgcaac gcgcaggcca catcgcagag
     5821 cctcacgggt gatgcacgca aatccttcat gaagacctgc ctgtcgagtg caccggcggc
     5881 ttcggattcc aagctcaact cccagcagct caagatgaaa cagtgcaatg cggatgccaa
     5941 ggccaaagga ctcacgggcg cggatcgcaa gaccttcatg agcagttgcc tcaagaaccc
     6001 cgcttcctga gccccgcttc cctcccccca gcgccgctct cggcgccgca tccgctaccc
     6061 caaccgcatc gcactgcgga cataatgagc cgcgcatcgg cctacggccg gttgctgagc
     6121 gcagcgcgca tctctacaat ttcagtgcgc tgctgtcgat caagaagaac caataatgca
     6181 cgctctgggc aggtacctga tcaggacgct tcatcacaga cggctcagtg attcgacggg
     6241 ggccgttgcg ggactggcga gcggcgtgtc cattctcgtt ggaatgatcg cagcccacta
     6301 tgcgccgcac ggaattcacc ggctggccgc ggtactgcac ttcgccagac aaccactgat
     6361 cgtcagaatg gcaccgatga tcgccggcgt cgccgccgct gtcgtcaccg ccgccgggat
     6421 catccggttt tactcatggt gcagggaacg ctacggagag gatgccccat gaccgttgga
     6481 cacgtgcgct ggtttcgaat caccaatcaa ttcttcgcct gcagccgttt tctgaaaggt
     6541 cagagtcgct ggggccccgt cgtcagaccg gtgcccagtc tttgccagcg gcagcgccga
     6601 gcgctcctga ggcgcaactg ccgctaactt gagcgcgctg cggtcctcac actctgtggc
     6661 cgggggacgt ggtactcgct caggatggtg tcgatggcag gtttctcttt ctcgagtaga
     6721 gcctggatac ggtcgcgcag ggccggatca ccgcgcctga cacccacaga gatgtcataa
     6781 accatgggcc attgcggtcc atcgagcgtc ggcgtcactt gctcgaggcg cagcggaacg
     6841 ggcgatcgac tggcgaagta gccggcgaac ggcccccaca ccagagccac atccacgtcg
     6901 ccgtgcgcca ccgcatcaac gatggccgag gccggattgg cagtttgata attgccgtag
     6961 agcatgtagc cccggacgtt ctggacgatg cctctgcgcg ccaaggcgtg cgcgggcggg
     7021 gtgttcatcg catctgcgcc gaccatctgt acgccgatcg tcagtcgctt gaggcgggcg
     7081 tcatccagtg tcaatccgct cagagggcgg tctgcccggg tcagaaactc ataggaagac
     7141 cgatagtagg gtcgcgtcgt ggcaagggaa tcaactccgg aggccacccc cggccagatg
     7201 tcacagagct cctcgcccag agtgtggcgc acgaagccg
//
LOCUS       DASRHN010000427         9894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_983584, whole genome shotgun sequence.
ACCESSION   DASRHN010000427 DASRHN010000000
VERSION     DASRHN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983584"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFB37_17260"
     CDS             <1..464
                     /locus_tag="VFB37_17260"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HZO24244.1"
                     /translation="ALVPLVAVLAITKGVETGLYRVLYAARRQTLYLIGLSTGTALIV
                     ILNIVLIPSLGLSGAVLAALISTTSVDIVCVAGLTKHFGPVFLPWALARLAIASAGTT
                     VLVLLLQHTALHPWLIAAIGCGIFPVAGVLLGLMPDPRHSHLFRQPDLAAH"
     gene            464..1711
                     /locus_tag="VFB37_17265"
     CDS             464..1711
                     /locus_tag="VFB37_17265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO24245.1"
                     /translation="MPNSTRPTPRVYWLTEEFSPPEMGGTGVMAAQLSRGIAERGIPT
                     VVITRQTRPPAARAEQIERVFVRRISPSGRMKGAGWKALPVMLMFLVRLMALLIAERR
                     RYDLVVASGMKIIPLAAVPICRWLRKGCIIRLESPFELVEPISAEALGTMGFAGRQLP
                     RVLRWFQHRALRGADRVVAISQEMETLVLQSGVAPERVRRIANGINMKKFHPVSEPER
                     RALRSQLGIPQDRTIVLFVGRLSRAKGVLMLIEAWPQMSGPHPELYLVIVGTGEGSWD
                     DCEEQVRGLVSSQGLEDRVVFVGRSEHADQYMQAADVYLFASEYEGFGLSVAEALSSA
                     LPSVLSAVGAAPELVDHGRNGFLFPAKNPLAMIEAFEECLAQRARWNEIGLAAREAVR
                     PYDLDRILQQYVELCRQAAPAAR"
     gene            1934..2764
                     /locus_tag="VFB37_17270"
     CDS             1934..2764
                     /locus_tag="VFB37_17270"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZO24246.1"
                     /translation="MGAKQLLLRYLSRKARPYLRTYGLLQTAKIFNKSAIVWEPGNQS
                     VLVLAPHMDDETIGCGGALAAHASRGSAVTVVFLTDCSRGGRLRGGEGGRESDPATPE
                     ELIERRRGEARRALAILGVQQMIFLGAPDGDLAPTPELVEQLHAILLDVRPSLIYLPC
                     FLEEHPDHRAVSDVLLAATESLPFEVQCLAYEVWTPLFPNCFVNIDGTMDAKRRAISE
                     YATQLADSDYLHTALGLNAYRSSALLGKSCSFAEAFLSVALSQYRHLYEDFRHAHAKT
                     "
     gene            2766..3344
                     /locus_tag="VFB37_17275"
     CDS             2766..3344
                     /locus_tag="VFB37_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZO24247.1"
                     /translation="MLSRRRFLAGAATLVVLVGAASWLRRSWRQPTARRLSSTERVTP
                     ALARVVVFLGALFGRELSEADQRDLSDRLAFAVAAEPRLAEDYEVLAAHLDRFGRKAG
                     ATEFLTASDAQRETVIDHVMRIGPPSLVERAWSHLVRSRKLYYQMRFETIPQLQWLYR
                     HSGVPWRARGYTRWQGIPGDWREYTRAGPPYP"
     gene            3341..4921
                     /locus_tag="VFB37_17280"
     CDS             3341..4921
                     /locus_tag="VFB37_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZO24248.1"
                     /translation="MISDKIYDLCIVGAGLAGGLLAAQATRKGHSVALLEAGSRFPFG
                     NRVGQLLHHQTLAGPRWPWLNEARDHYVDSSAASIGVGYALNSNRVKGVGGSTLHWDG
                     RINRLMPVDFAAATTNGLGVDWPITYAELEPWYSQADWELGVSGTWHRAMPPRSRDFP
                     MEGFPMSVDDRLWEPIAERLGIEVFATPWAINSRPFGGYSQCLAFAACQICPSGARYS
                     AERHIAIAEGTGRCELAPETVARRIDLGPSGKVKAVHATRLTGEQVEVRARVYVIAAH
                     AVESARLLLLSRVGNHSDQVGRNLMEHVYVDAGGFLPSRRFYPGRVGYEVTESLSYYR
                     AEEKGRRGGIKLEFTFDEDPLHESESRHLWGSRLAQYDRERFGHWIGLEAETEVQPNP
                     DSRVTLDGTQKDLFGDAVPNIRFAFSPVDARTQKEAGEILANLLHEVGATEVSQEPLG
                     ATSWGAHHMGTCRMSVDPDKGVVDRDCRVHGTSNLYVAGSSVFPTSGALQPSLTIAAL
                     SLRLAEHLQQVLSSPQAA"
     gene            complement(4863..6479)
                     /locus_tag="VFB37_17285"
     CDS             complement(4863..6479)
                     /locus_tag="VFB37_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24249.1"
                     /translation="MQFGILLDRPQLKRWQLNCLQRLLGLPQVQPVCLIQVGGPTTKL
                     SRGPAPSEIPADDGTLEAVLPLPAALEALSGAIPSGLDFVLSFASAPAAAALREQARW
                     GVWRYQFGDWTRLRGGPPGFWEVHYGDPESAVLLVRETPDPDAVVVLREGYVRTLAQS
                     VARNRAQLLSVAVHFAAQMCLDLQNGVVEHMSTATRRSGAPAHGDPTSLQRAICTLRI
                     LGRMAAEGYHALFTHTQWNVGIVEQPITAFVNGARPTSVRWLPAPKRNEFRADPFGVL
                     RDGHLTILLEDFSYISNRGTISATQPAAGTTVPVTIGPQPAVHLSYPYLIEVEGRLYC
                     MPEAHEAREVVLYEVKRFPDDWARVARFPIETVIVDATLFRHEDRWWIAGSEPGTKGA
                     SSELHLWYAPAIEGPWTAHPGNPVKIDIRSARPAGTPFYRDGQLYRPAQDCSRSYGGR
                     VSINHVLALTPTAFQEVVVATVEPAATGLFREGIHTLSSVGAITLIDGKRTLFVPEEF
                     QRNARHFLRQALRRLGVSSKPAAGVPPVAS"
     gene            complement(6485..7600)
                     /locus_tag="VFB37_17290"
     CDS             complement(6485..7600)
                     /locus_tag="VFB37_17290"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZO24250.1"
                     /translation="MRIAVMLRHYDQHGGGVRVYTRALLRQLIEHHPEHEYILLFNNP
                     ALTSGFRDRPQVREMVVPGGSVLLWDQVGVPRAVRKLGIDVLFNPKYSIPLSGRYPAS
                     WVCHGLDWYVMPWASRWVDRLSHRYLVPRYARRAAAIVAVSEITREHVHEFLRVPLDR
                     IHTVYTGIEAAYNHTVPEDARQAIRNKFNLPQRYVLYSGAVYPPKNFTRLIQAYAQVG
                     PALGVSLVIAGGENRFLSEQELKEPERQNLGEWVRWAGWVDPQTLPAFYQMAEALLLP
                     SLFESFGLPIVEAMASGCPVVTSNCYGTREIAGDAAILVEPRSVEAIAEGIQRALTDS
                     TLRQQLIAKGREHARPFTWERCAAQTLQVLERIAHPG"
     gene            7719..8834
                     /locus_tag="VFB37_17295"
     CDS             7719..8834
                     /locus_tag="VFB37_17295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZO24251.1"
                     /translation="MRRGLSLYLDLLRFLLALTVMLGHATEPGYIGRLSEVWTFPHYG
                     LTAVMGFFVLSGFVIAHVTQGSESRPTDYFAARAARMYSIVIPALLLTALLGVAGAFI
                     NPETYLHGPIHVGAHQPRRYFLTALFAQDFWIWRREMTPGINHPFWSLSYEVTYYVLF
                     GLTLVRRPRVAVLGGLALLCLAGPKIAALLPIWVLGVLAYRGTRRFTLPAPLCAVLMI
                     GTLCGLYVTGTYRGADDPVNRYTLDYLEGFLFAVNIIAAAGLAPWLERALGWCPRFIR
                     WLGMLTFALYLCHRPLMYFLSAVPMGAPGEWRQSAWLFGMTLAVVIGIAHLGEWFRGG
                     LRRSLTRLLPQIPLHRAPLAATPGCLPGSSSGTTAPR"
     gene            complement(8734..>9894)
                     /locus_tag="VFB37_17300"
     CDS             complement(8734..>9894)
                     /locus_tag="VFB37_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HZO24252.1"
                     /translation="GGLDSSLLVAMLAKRVGVKNLPTFTVGLPHPRFDEAPYARAVAQ
                     EYGTEHHERSLSPSLIALLPDLIYHLDEPSDPLSLCTYHVAELAQRYVKVVIGGDGGD
                     ELFGGYDRYYGNLYAGHYARVPAALRRHIFGPALSLIPEAGWYKSLGHQLRWLQRLSF
                     LSGGDRYAASLSYFYFDPAMRAALFTPEARAHLERADAEAAIRVPYDAAHGDPLDHML
                     YADSQVRLPDHPVMISDRMSMAHGLETRSPFMDHRLAEFAARLPSSMKMRGRSLRWAQ
                     RKLAARYLPREVLERPKQGFSSALPYLLQQEYRLLYRHFLPDSQLVAAGILQRGPIDE
                     LLRAHLAGRADHGNRLWLLINAELWYRMMIQGGSRESLRAELDAAGFAAAAA"
BASE COUNT         1634 a         3181 c         3227 g         1852 t
ORIGIN      
        1 ccgccctggt gcccctggtc gcggtgctcg ccatcaccaa gggagtcgaa accggtctgt
       61 atcgtgtttt gtatgcagcg cgacgccaga cgttgtatct catcggactg agcaccggca
      121 cggcgctcat cgtgatactc aacatcgtgt tgatcccctc gctcggcttg agcggcgcgg
      181 tgctggcggc gctgatcagc acgacaagtg ttgacattgt ctgtgtcgct ggcctgacga
      241 agcacttcgg tcccgtcttc ctgccgtggg ctctcgcgcg cctggcgatt gcgagtgccg
      301 gaacgactgt cctcgtgctg ctgttgcagc acacggctct gcatccttgg ctgatcgctg
      361 cgatcggctg cggcatcttc ccggtagccg gcgtgctgct cggcctgatg cccgacccgc
      421 ggcacagtca tttgttccgg caacctgacc tggcggcgca ctgatgccca attccacgag
      481 gccaacaccg cgcgtctact ggctgaccga agagttctct cctccggaga tgggcggcac
      541 cggagtcatg gcggcgcagc tgtcgcgcgg catcgcggag cggggtattc ccaccgtcgt
      601 cattacgcgc cagacccggc cgccggcggc aagggctgag cagatcgagc gcgtgttcgt
      661 gcgtcgcatc tcaccgagcg gccgcatgaa aggcgcgggc tggaaggcgc tgcccgtcat
      721 gctgatgttt ctcgtgcggc tgatggcgct gctcattgca gagaggcgcc gttatgacct
      781 cgtggtggcg tccgggatga agatcattcc gctcgcggcg gtcccgatct gccgctggct
      841 gcgcaagggc tgcatcatcc gcctcgagtc accgttcgag ctggtggaac cgatctccgc
      901 tgaggcgctc gggaccatgg gctttgctgg gcggcagctg ccgcgggtgt tacgttggtt
      961 tcaacaccgg gcgttgcgag gtgcggaccg cgtcgtcgcc atctcgcagg agatggaaac
     1021 gctcgtcctg caaagtgggg ttgcgccaga gcgggtccgc cgcatcgcca acggcatcaa
     1081 catgaagaag ttccacccgg tgtcggagcc ggagcgccgt gcgttgcgca gccagctcgg
     1141 cattccgcaa gatcgcacga tcgttctctt cgtcggtcgg ctgtcgcgcg ccaagggagt
     1201 gctgatgctc atcgaggcct ggccgcagat gtccggtcct catccggagc tctacctggt
     1261 gatcgtggga accggcgagg gctcctggga tgactgcgag gagcaggtac gagggctcgt
     1321 gagctcccag ggcctcgagg atcgcgtggt ttttgtcggg cggagcgagc atgcggacca
     1381 gtacatgcag gcggccgatg tgtacctctt tgcgagtgag tacgagggat ttgggctttc
     1441 ggttgccgaa gcgctctcca gcgcgctgcc ttcggtactc tcggcagtcg gcgccgcacc
     1501 ggagctggtc gatcatggcc gcaacggctt cctgtttccc gcgaagaatc cgctcgcgat
     1561 gattgaagcg tttgaggaat gtctcgcaca acgcgcgcgt tggaatgaga tcgggctggc
     1621 cgcgcgcgag gcggtgcgtc cgtacgatct cgatcgcatc ctgcagcagt acgtcgagct
     1681 ttgccgccag gctgctccag ctgcgcgttg atccaagtta gaagcgccgc tagctttcaa
     1741 caacctttcg tcgcagctgc ccgcgcgagt tcttcatgga tctttgcgcg ccactcgctc
     1801 atttatgttg agcgagcccg caatctttgt gaactgcgtg cacaatatgc acaaagcgat
     1861 tcactacact actccccaac accaagcaga gcgggcaaga gaaacccgcc ccggtacgca
     1921 gctcgcgagc ctcatgggcg ccaagcaact tcttctgaga tacctgagcc gtaaggcgcg
     1981 tccttatctg cgcacctacg gcttgttgca gaccgcaaag atcttcaaca agtctgcgat
     2041 cgtgtgggag ccgggcaacc agtccgtcct cgtgctggcc cctcacatgg atgatgagac
     2101 catcggctgc gggggcgccc tggccgcgca cgcatcgcgc ggcagcgccg tgacggtcgt
     2161 gttcctgacc gattgctccc gcggtggccg ccttcgcggc ggcgagggcg gccgtgaaag
     2221 cgatccggcc acgccggaag agctcatcga gcgacggcgc ggcgaggcgc ggcgggccct
     2281 cgcgatcctg ggtgtgcagc agatgatctt ccttggcgct ccggacggtg atctggcacc
     2341 gacgcccgag ctggttgagc agctgcacgc catcctgctg gacgtgcggc cctcgctcat
     2401 ctacctgccg tgctttctcg aagagcatcc cgatcatcgc gcggtcagcg atgttctgct
     2461 cgccgccacc gagagcctcc ccttcgaggt gcaatgcctg gcgtacgagg tctggactcc
     2521 cctctttccc aactgttttg tgaatatcga cggcacgatg gacgcgaaac gtcgcgcgat
     2581 ttcggaatac gccacgcagc tggcggattc ggactatctg cacaccgccc tggggctcaa
     2641 cgcctaccgc tcctcggcgc tgctgggcaa gtcctgcagc tttgccgagg cgtttctctc
     2701 tgtggcattg tcccagtaca ggcatctgta cgaggacttc cgccacgcgc acgccaagac
     2761 ctagcgtgct ctcccggcgt cgcttcctgg caggggccgc tacgctcgtg gtccttgtgg
     2821 gtgcggcaag ctggctgcgc cgaagctggc gccagcccac ggcgaggcgc ctctccagta
     2881 ccgagcgcgt gacgcccgct ctggcccgag tcgtggtgtt tctgggggcc cttttcgggc
     2941 gcgagctctc ggaagcggac cagcgagacc tttcggaccg gctcgccttc gcggtagccg
     3001 ccgaaccgcg cctcgccgag gattacgagg tgctcgccgc gcacctggac cgctttgggc
     3061 gcaaagccgg ggcgaccgag tttctgaccg cgagtgatgc gcagcgtgag acggtcatcg
     3121 atcacgtgat gagaatcggg ccgccctctt tggtggagcg tgcgtggtcc cacctagtgc
     3181 gcagccgcaa gctgtactac cagatgcgtt tcgagacgat tccccagctt caatggctct
     3241 accggcattc gggtgtgccc tggcgagccc gcggctacac ccgatggcag ggcataccgg
     3301 gagattggcg tgagtacacg cgcgccggtc cgccgtatcc atgatatcgg acaagattta
     3361 cgacctctgt atcgttggag cggggttagc gggtggcctg ctcgccgccc aagccacgcg
     3421 caaaggtcac tccgtagcac tcctcgaggc cggtagtcga ttcccattcg gcaaccgtgt
     3481 cggacagctt ctgcatcatc agacgctggc aggcccccgc tggccgtggc tcaacgaagc
     3541 ccgcgatcat tacgtggact cctccgcggc ctctatcggc gtgggctatg cgctgaactc
     3601 caatcgggtc aaaggcgtcg gcggatcgac gctgcactgg gacgggcgga tcaaccgcct
     3661 catgcccgtg gattttgccg cggccacgac caatgggctg ggcgtggact ggccgatcac
     3721 ctacgcggaa ctcgagccct ggtactcgca ggccgactgg gagctgggcg tctcgggcac
     3781 gtggcatcgg gcgatgcccc cgcgcagccg cgacttcccg atggaaggct tcccgatgtc
     3841 ggtagacgat cgcctctggg agccgatcgc cgagcggctc ggcatcgagg tgtttgcgac
     3901 accctgggcg atcaactcgc gcccctttgg cggctactcg cagtgccttg cgtttgcggc
     3961 gtgtcagatc tgtccgagcg gagcgcgtta cagtgccgag cggcatatcg ccattgccga
     4021 gggcactggg cggtgtgagc ttgcgcccga gacggttgcg cggcgcatcg atctcggccc
     4081 ctccggcaag gtgaaggccg ttcatgcaac gcggttgacg ggcgagcagg ttgaagtgcg
     4141 cgcacgggtt tatgtcattg ccgcgcacgc ggtggaatcc gcgcggctgc tgctgctgtc
     4201 cagggtcggt aaccactctg atcaggtcgg tcgcaatctg atggagcatg tctacgtcga
     4261 cgcgggcggc ttcctgccgt cgcgccgctt ttatcccggc cgggtgggct acgaagtcac
     4321 cgagtctctc tcgtactatc gcgccgagga gaagggccgg cgcggtggca tcaaactcga
     4381 gttcaccttc gatgaggatc cgctgcatga gagcgaatcc cgccacctct gggggtcgcg
     4441 cctcgcccag tacgatcgcg aaaggttcgg ccactggatc ggactcgagg ccgaaaccga
     4501 ggttcagccc aatcccgaca gtcgcgtcac cctggatggc acccagaagg atctgttcgg
     4561 agatgccgtc ccaaacatcc gcttcgcctt cagcccggtc gatgcccgca cacagaagga
     4621 agccggcgag attctcgcga acctgctgca tgaggtcgga gccaccgagg tctcgcagga
     4681 gcccctgggc gccacctcat ggggcgcgca ccacatggga acctgtcgca tgtcagtcga
     4741 tcccgataaa ggtgtcgttg atcgggattg ccgtgtccac ggcacgagca atctgtacgt
     4801 cgccggcagc agcgtgttcc ccacctcagg cgcgctgcaa ccgagcctga ccattgccgc
     4861 cctcagcttg cgactggcgg aacacctgca gcaggttttg agctcacccc aagccgcctg
     4921 agggcctgcc gcagaaagtg ccgcgcgttg cgctgaaatt cctcgggcac gaacagcgtc
     4981 cgtttgccgt cgatcagcgt gatcgcgccg acgctcgaga gagtatgaat gccctcgcga
     5041 aacagacccg tcgcggcggg ctcgaccgtt gcgacgacca cttcctgaaa agccgtcgga
     5101 gtcagggcca gcacgtggtt gatcgagacg cggccgccgt aactcctcga gcagtcctga
     5161 gcagggcggt agagctgccc atcacgatag aagggtgtcc cggcaggtct cgccgaacga
     5221 atgtcgatct tcacggggtt gcccggatgg gccgtccagg ggccctcgat tgctggcgca
     5281 taccataggt gcaactcgct gctcgcccct ttggtcccgg gctcggagcc cgcgatccac
     5341 cagcgatcct cgtgacggaa cagcgtcgca tcgacgatga ccgtctcgat cggaaagcgc
     5401 gccacgcgcg cccagtcatc cgggaaacgt ttcacttcgt agagcacgac ctcgcgcgcc
     5461 tcgtgcgcct caggcatgca atacagccgg ccctccacct cgatgagata cggatacgaa
     5521 aggtgaaccg caggctgcgg cccgatggtg accggcacgg tcgtgcccgc ggccggctgc
     5581 gtggcggaga tcgtcccgcg gttgctgatg tagctgaagt cctccagcag gatcgtcaga
     5641 tggccatcgc gcaggactcc gaagggatcg gcgcggaact cgtttctctt cggggccggc
     5701 agccagcgca cggaagtggg ccgcgcgccg ttcacgaagg ccgtgatggg ctgttcgacg
     5761 atgcccacgt tccattgcgt gtgcgtgaag agagcgtggt aaccctctgc ggccattctt
     5821 cccaggatcc ggagcgtgca gatcgcccgc tgcagcgatg tcggatcgcc atgcgctggg
     5881 gcgccgctgc gccgagtcgc ggttgacatg tgctcgacaa cgccgttctg cagatcgagg
     5941 cacatctgag cggcgaagtg cacggcgaca ctgaggagct gggcgcggtt cctggccaca
     6001 gactgcgcga gagttcgcac atagccttct cgcaacacga ccaccgcgtc cggatcaggc
     6061 gtttcgcgca ccaacaggac cgctgactcg ggatctccgt aatgcacctc ccagaatccg
     6121 ggcgggccgc cgcgcagtcg ggtccagtcg ccgaactggt atcgccacac tccccagcgt
     6181 gcctgctcgc ggagtgcggc cgcggcgggg gcgctcgcaa agctgaggac gaaatcgaga
     6241 ccggacggaa ttgctccgct cagtgcttcg agcgccgctg gcagcggcag taccgcctcg
     6301 agcgttccat cgtccgccgg gatctcactc ggtgccggcc ctcgactcaa cttcgtcgtc
     6361 gggccgccca cctggatgag gcagaccggc tgcacctgtg gcagccccag cagccgctgc
     6421 aggcaattga gctgccagcg cttcagttga ggacggtcaa gaaggattcc gaattgcatg
     6481 ctcatcaacc cgggtgcgcg atccgttcca ggacctgtag ggtttgtgcg gcgcaccttt
     6541 cccaggtgaa aggtcgggca tgctcgcggc ccttggcgat gagttgctga cgcagggttg
     6601 aatcggtcag ggcccgctgg atgccttcgg cgatcgcctc caccgagcgt ggctcgacga
     6661 ggattgccgc atcgccggcg atctcacgtg tgccgtagca gttggacgtg accacggggc
     6721 agcccgaggc catggcttcc acgatcggca ggccgaagga ctcgaacagt gagggcagca
     6781 gcagcgcttc ggccatctga tagaaggccg gcagggtctg cggatccacc cagcccgccc
     6841 agcgtaccca ctcgccgagg ttctgccgct ccggctcctt cagctcctgc tccgacagga
     6901 accggttctc gcctcctgcg atcacgagcg acactccgag cgcgggccca acttgcgcat
     6961 aggcctggat caggcgggtg aagttcttcg gcgggtagac tgcaccggaa tagagcacgt
     7021 agcgctgcgg gaggttgaac ttattgcgga tcgcctggcg tgcatcttcc ggcacggtgt
     7081 ggttgtaggc tgcctcgatc cccgtataca cggtgtgaat gcgatcgagc ggcacccgca
     7141 gaaactcgtg gacgtgctcg cgcgtgattt ccgacaccgc cacgatggcc gccgcacgtc
     7201 gtgcatagcg cggcaccaga tagcggtggc tcagccgatc gacccagcgg gaggcccacg
     7261 gcatcacata ccagtccagg ccgtgacaca cccaggaggc ggggtagcgc ccactcagtg
     7321 ggatggagta tttcggattg aacagtacgt cgatccccaa cttgcgcacc gctcggggaa
     7381 cccccacctg gtcccacagc agcaccgagc cgccgggcac gaccatctcc cgcacttgcg
     7441 gccggtcgcg aaagccactt gtcagggcag ggttgttgaa gaggaggatg tactcgtgct
     7501 cgggatggtg ctcgatgagc tgacgaagca gggcacgggt atacacgcgc accccgccgc
     7561 cgtgctggtc gtaatgccgc agcatgactg cgattcgcac tgccctcggc cctccttaag
     7621 aaaaatctac ttgccctgcg cgtgcgcgca gcgccaaatg ggcttgcgcc cccttactca
     7681 gctcgcgcag gttgcgaaaa caacaacagc ggcaccccat gcggcgtggt ctgtcacttt
     7741 acctggatct gctgcgtttc ctgctggctc tcacggtgat gctgggtcat gccaccgagc
     7801 ccggctatat cggccggctg tccgaggtct ggaccttccc gcactatggg cttacggccg
     7861 tgatgggatt cttcgtgctg tcgggtttcg tgatcgcgca cgtcacgcag gggagcgaga
     7921 gcaggccgac ggattatttc gctgcgcgcg ccgcacgcat gtattccatt gtcattccgg
     7981 ccctgctgct gacggcgctg ctcggagttg ccggagcatt catcaatccg gagacgtatt
     8041 tgcatggccc gattcacgtg ggggcgcatc aaccccgtcg gtattttctc acggcgctgt
     8101 ttgcgcagga cttctggatc tggcggcggg aaatgacacc cggcatcaat catcccttct
     8161 ggtcgctgag ctatgaggtg acctattacg tgctcttcgg cctgactctc gtgcggcgac
     8221 cgcgggttgc cgtgctgggg gggcttgcgc tcctgtgtct cgctgggccg aagatcgcag
     8281 cgttgctgcc catctgggtt ctcggtgtgc tcgcttaccg ggggacgcga cggttcacgc
     8341 tgcctgcacc actgtgcgca gtactgatga ttggaacgct ctgcggcctc tacgtcaccg
     8401 ggacctaccg tggggccgac gatccggtca accgctatac actggactac ctcgaagggt
     8461 tccttttcgc ggtcaacatc atcgccgcgg cggggctcgc tccgtggttg gaacgcgcac
     8521 tcggctggtg tccgcgcttc atccgctggc tcggcatgct gaccttcgcg ctttatctgt
     8581 gtcaccgtcc gttgatgtac tttctttcgg cggttccgat gggcgctcct ggggagtggc
     8641 gccagagtgc atggctgttc ggcatgacgc tcgccgtcgt gatcgggatc gctcatctgg
     8701 gagagtggtt tcgcggcggt ctgcgacgca gcctcacgcg gctgctgccg caaatcccgc
     8761 tgcatcgagc tccgctcgca gcgactcccg gctgcctccc tggatcatca tccggtacca
     8821 cagctccgcg ttgatcagca gccagaggcg gttgccatgg tcggcccggc ccgccagatg
     8881 ggcccgcagc agctcatcga tcggcccgcg ctgcaggatg cctgcggcca cgagctgcga
     8941 atccgggagg aaatgccggt agagcaaccg gtattcctgt tgcagcagat agggcagggc
     9001 cgaggagaag ccctgcttgg gccgctcgag gacctcacgc ggcaggtaac gcgcggccag
     9061 tttgcgttgt gcccaccgca gtgagcggcc gcgcatcttc atactcgagg gcaatcgggc
     9121 cgcgaattcc gccagtcgat gatccatgaa cgggctgcgt gtctcgagcc cgtgcgccat
     9181 cgacatccga tcgctgatca tgacggggtg atcgggcagc cggacctgac tgtccgcata
     9241 gagcatgtga tcgagcggat cgccgtgggc cgcatcgtac ggaacgcgaa tggcggcctc
     9301 ggcatcggcg cgctccaggt gcgcgcgcgc ttcgggcgtg aagagcgcgg cgcgcatcgc
     9361 gggatcgaag tagaagtagc tcaagctcgc cgcatagcgg tcgccgccgg acaggaacga
     9421 cagccgctgc agccagcgca gctgatggcc gagactcttg taccaaccgg cttcggggat
     9481 cagcgacagc gccggcccga agatgtgacg gcgcagcgcc gcgggcacgc gcgcgtaatg
     9541 gcctgcgtag aggttgccgt agtagcggtc ataaccgcca aacagctcat cgccgccatc
     9601 gcctccgatc acgactttca cgtatcgctg ggcgagctcg gcgacatggt aggtgcacag
     9661 tgagagcgga tccgagggct catccaggtg atagatgaga tcgggcagca gtgcgatcag
     9721 cgaaggggac aggctgcgct cgtgatgctc ggtaccgtat tcctgcgcca ccgctcgggc
     9781 atacggggcc tcgtcgaagc gcgggtgggg caggccgacg gtgaaggttg gaagattctt
     9841 cactccgacg cgcttggcga gcatcgccac gagcagactc gaatcgaggc cgcc
//
LOCUS       DASRHN010000428         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1809949, whole genome shotgun sequence.
ACCESSION   DASRHN010000428 DASRHN010000000
VERSION     DASRHN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809949"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFB37_17305"
     CDS             <1..369
                     /locus_tag="VFB37_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZO24253.1"
                     /translation="LERVICRVADLEPAGARGFTIGEGDWPLRGVVVRVRDEIRGYIN
                     RCPHAGHPLNLLPNRFLTPDGSLLLCASHGALFEKLTGLCIAGPCPGQSLQTIALKTI
                     DGFVMLADGVDAAALANAVR"
     gene            complement(500..811)
                     /locus_tag="VFB37_17310"
     CDS             complement(500..811)
                     /locus_tag="VFB37_17310"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HZO24254.1"
                     /translation="MTAPLPSIGEWYRHSGGELFEVVAFDEDDGTIEIQYFDGTVEEM
                     DTEDWEGQWADRALEAAEPPEDWSGSVDVEVGDDAGGGTDLSDEGGLRASSLDGIDLF
                     E"
     gene            1001..1633
                     /locus_tag="VFB37_17315"
     CDS             1001..1633
                     /locus_tag="VFB37_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZO24255.1"
                     /translation="MLEQPLKFFVVFFVVVEPVSLIPMFSELTMGATPAYKRRMALKA
                     VLISAFILVLFALCGASLLELMGISLEAFRIFGGLLLFLLALEMVFARESGTRTSTDE
                     QAESRRRADISVFPIAFPFIAGPGALATILLWFGPLHLPSQTALFIGLLVAVAAVLAI
                     SLALMWLSEPLMRVLGVTGANVAGRLSGVILGALAVQFVIDGLQHTFSSR"
     gene            complement(1680..2378)
                     /gene="trmB"
                     /locus_tag="VFB37_17320"
     CDS             complement(1680..2378)
                     /gene="trmB"
                     /locus_tag="VFB37_17320"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZO24256.1"
                     /translation="MSAQEPGADGIRSVRSYVVRGGRVTEAQERALRSLWPKYGLAFA
                     PMPLDLDVTFGRQAPRSVEIGFGNGDNLAALAAAHPERDYLGIEVHRPGVGRLLLTLE
                     EAGRGNVRLICHDAVEVLDRQIGSQSLDEVLILFPDPWPKKRHHKRRLLQGPFVELVA
                     DRLRVGGVLRAATDWRPYAEAALEVLSACPGLHNLSADNTFVPRPTERRPTRFEVRGE
                     RLGHEVWDLAFARQ"
     gene            complement(2403..2777)
                     /locus_tag="VFB37_17325"
     CDS             complement(2403..2777)
                     /locus_tag="VFB37_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZO24257.1"
                     /translation="MRLSKMPHTTPDQITEFEPDEVKELLEAGRILLIDVREPAEFAA
                     ERIQGALLYPLSTFDVTRLPPDEPRQVVFQCAAGGRSLTAARERLAHGQPGAHMAGGI
                     SEWKAMGLPTIRIDPRTGRAIQ"
     gene            2954..3517
                     /locus_tag="VFB37_17330"
     CDS             2954..3517
                     /locus_tag="VFB37_17330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO24258.1"
                     /translation="MPDRAPPTDQEARLQELLDRCAARDAGALQPLYELAAPLLFGCL
                     TRILRRRALAEEALQDVFVTIWQRAAQFSAARGKPMTWMISIARYRAIDLLRHERSAP
                     VLVRELPEQSSEDEPSHELGAWWSGSALLERCLALLTDRQRNCLELAFVGGNSHEDIA
                     RLTGNPLGTVKSWIRRALQSLRQCLEE"
     gene            3519..4196
                     /locus_tag="VFB37_17335"
     CDS             3519..4196
                     /locus_tag="VFB37_17335"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZO24259.1"
                     /translation="MTPRDKRLVDPLAAEYVLGTLRGPARRRFERWRASSALVAQRCE
                     FWEEQLISLAREVRPIQPPPHLWQGIRQRLNLPQPRWRPAYALAIAASVLIVIGLSLV
                     LYWRSIQPGPPSELATIAAPSGAAAWQVEVYSQRGQMIVRAGQLPARPANRDFELWAL
                     PQGGKPVSLGVLPVTGTVQHGLTGTQQEALAHATQIAVTVEPSGGSPTGQPSGTPVFI
                     APLRAIS"
     gene            complement(4271..>4859)
                     /locus_tag="VFB37_17340"
     CDS             complement(4271..>4859)
                     /locus_tag="VFB37_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZO24260.1"
                     /translation="PVNSLTEPGTPNPKSAPGFHAKASLTLPIGGGSDKLYLSTAGIV
                     QKQNYDVSGVAGDFSSRGIDFFAKLDVGGLSLFGYYYTGDGIGTTALFVLADDGLGHA
                     RKSDGYLVQATYTMGPVKLGVNYGSSRLDFGSAADGFNTPNLLDNNNKVTGGIYYSLT
                     KNLTLLGEISHVKTLAHNDFENTSNNFNVGSYLSF"
BASE COUNT          793 a         1592 c         1564 g          910 t
ORIGIN      
        1 ctcgagcgtg tcatctgtcg tgtggcggac cttgagccgg cgggggctcg tgggttcaca
       61 atcggcgagg gggactggcc gctgcgcggt gtcgtggtgc gcgtccgcga cgagattcgc
      121 ggctatatca atcggtgccc acatgccggg catcctctga atcttctgcc gaaccgtttc
      181 ctgacaccgg acggttcgct gctgctgtgc gcctcgcacg gcgcgctgtt cgaaaagctt
      241 acgggtttgt gcatcgcggg cccgtgcccg ggccagtctc tgcagacgat tgcgttgaag
      301 actatagacg gcttcgtgat gctggcggac ggggtggatg cggccgccct ggccaacgcg
      361 gtgcgctgac gattcccgag cgctgacgat tcccgagcgc tgacgactgg cgcgagaaat
      421 ccggatccca cgagcccgcc catcgacggg ccgctaggcg ggcggtcctc ttgtgctcgg
      481 agcgaattgc tgcgccgcgc tactcgaaca gatcgatgcc gtcgagtgaa ctcgcgcgca
      541 agccgccctc atccgagagg tcggtgcccc cgccggcgtc atccccgacc tcgacgtcca
      601 ccgagccaga ccagtcttcg ggaggctccg cggcctcgag tgcgcggtcg gcccactggc
      661 cctcccagtc ctcggtgtcc atctcttcga ccgtaccatc gaaatattgg atctcgatcg
      721 tgccatcgtc ctcatcgaac gccacgacct cgaacaactc accgccactg tggcgatacc
      781 actcgccgat gctgggaaga ggggccgtca tcttgcaacc tccggaaagc cgctccttcg
      841 ggcttcatgc cgagcacgcc cgggtgagcg cgtacgtcgc actgcttcgc gggcccgctg
      901 ggcgctgtta tgctcccggc gcgcgtactc tcagtgccgc tcgatgttgc accggacgtg
      961 tgacgctttc aagaagttat tttgctctca ccggggtgcc atgctcgagc agccgttgaa
     1021 gttcttcgtc gtattcttcg tggtcgtgga gccagtctcg ctgatcccga tgttcagcga
     1081 attgacaatg ggtgcgacgc cggcatacaa gcggaggatg gcactgaagg ccgtgctcat
     1141 ctcggccttc atcctggtgc tattcgcatt gtgcggcgcc tcgttgctgg agctcatggg
     1201 catcagcctc gaggcgttcc gcatcttcgg cggcttgctc ctattcctgc tcgcgctcga
     1261 gatggtgttt gcacgcgagt cgggcacgcg cacctcgaca gatgagcagg cggagagccg
     1321 ccgccgcgcc gacatctccg tgtttcccat cgcctttccc ttcattgcgg gccccggcgc
     1381 cctcgcaact attctgttgt ggttcggtcc actgcatctg ccctcacaga cggcactgtt
     1441 catcggcttg ctggtggccg ttgcggccgt gctggcgatc tcgcttgcgc tgatgtggct
     1501 ttccgagccg ctcatgcgcg tgctcggcgt cacgggtgcc aatgtcgcgg gccggctctc
     1561 cggtgtgatt cttggcgccc tggccgttca attcgtgatc gacggcttac agcacacgtt
     1621 ctcgagccgc tagctgcgcg ctgcaaccgc tgtcgttctc acgagttgat cgctggccgc
     1681 tactgccgtg cgaaggccag atcccagacc tcatggccca gccgctcgcc gcgtacctca
     1741 aaacgcgtgg ggcggcgctc ggtgggacgc ggaacgaaag tgttatccgc cgagaggtta
     1801 tgcagaccgg gacaggcgct caacacctcg agagcggcct cggcgtaggg acgccagtcg
     1861 gttgccgcgc gaagcacgcc tccgacacgg agccggtccg cgaccaactc gacgaacgga
     1921 ccctgcaaca gtcggcgctt gtggtggcgc ttcttcggcc acggatcggg aaagagaatg
     1981 agcacctcat cgaggctctg cgagccaatc tggcgatcca gaacttcgac cgcgtcgtgg
     2041 cagatgagcc gcacattgcc tctaccggct tcctccaggg tcaacagcaa tcgcccgact
     2101 ccaggacgat ggacctcgat ccccagatag tcccgctcgg ggtgcgctgc agcgagcgct
     2161 gccaggttgt caccgttgcc gaagccgatc tctacgctgc gcggagcttg gcggccgaaa
     2221 gtgacgtcca gatcaagcgg catgggggcg aatgcgaggc catacttcgg ccacagtgag
     2281 cgtagcgccc gctcctgcgc ctcggtgacg cgtccgcctc gcacgacata ggagcgaact
     2341 gagcggatcc catcggcacc cggttcctga gctgacatca cccctccccc gcgacggggt
     2401 gctcactgga tggcacggcc cgtgcgcggg tcgatgcgaa tggtcggaag ccccatcgcc
     2461 ttccactccg agatgccacc ggccatgtgg gcgcccggtt gaccgtgtgc cagccgctcg
     2521 cgtgcagcgg tcagcgagcg accgcccgcg gcacactgaa acacgacttg gcgcggctca
     2581 tccggaggaa gccgcgtgac atcgaaagtg gacaacggat agagcagggc gccctggatg
     2641 cgttctgcgg cgaactccgc cggctcgcgg acatcgatga gcaggatacg gcccgcttcc
     2701 agcagctcct tgacctcgtc gggctcgaat tcggtgatct gatcgggtgt ggtgtgaggc
     2761 atcttgctca atcgcatgtg ggcctgtcgg tcttgcaatt ctaccaaccg agtcccgcgc
     2821 ctgccgcatc cgaaagtgtg gctggaccgt attgccctga agctccggcc ctcgtctaag
     2881 gcaaaagcag cgaggcccga gactacacta caccgcatta caatgggtga atcaggccgt
     2941 acgagtgacg cacatgccgg accgcgcccc gccgaccgac caggaagcac gtctgcagga
     3001 gctgctcgat cgctgcgcgg cgcgggacgc aggtgctctg caaccgctct atgagctcgc
     3061 cgcaccgctc ctgttcggct gcctgactcg catcctgagg cgccgcgccc tggccgaaga
     3121 ggctctccag gatgtgttcg tcacgatctg gcagcgagcg gcccagttca gcgccgcgcg
     3181 cggcaagccg atgacgtgga tgatctccat cgcccgctat cgggccatcg atctgctacg
     3241 gcacgagcgt tctgcacccg tgctggtccg agagctaccc gaacagtcat cggaggatga
     3301 gccctctcat gagctgggcg cctggtggtc cggatcggca ctgctcgagc gttgcctggc
     3361 gctcctcacc gatcggcagc gcaattgtct ggagctcgcg ttcgtcgggg gcaactctca
     3421 cgaggacatc gcgcgtctga ccggaaatcc gctgggcacg gtcaagagct ggatccgacg
     3481 cgcactgcag agtctgcggc aatgtctgga ggaatgaggt gacgccccgg gacaaacgtc
     3541 tggtcgatcc acttgccgcc gagtacgtgc tcgggacact gcgtggaccg gcccgccggc
     3601 gcttcgagcg ctggcgcgca agctcggcgc tcgtcgcgca gcgctgcgag ttctgggagg
     3661 agcaactcat ttcgctcgca cgcgaggtgc gtccgatcca gcccccgccc cacctgtggc
     3721 agggcattcg gcagcgcttg aatctgcccc agccgcgctg gcgtccggct tatgcgcttg
     3781 cgatcgccgc cagcgtgttg atagtcatag gactgagtct cgttctgtac tggcgcagca
     3841 tccagccggg gccgccctcc gaattggcga ccatcgcggc accttccggt gccgccgcgt
     3901 ggcaagtcga ggtgtacagt cagcgcgggc agatgatcgt gcgcgccggt cagcttccag
     3961 cgcgaccggc gaatcgagac tttgagctgt gggcactgcc gcagggcggc aagcccgttt
     4021 cactcggtgt gctgcccgtg accggcaccg ttcaacatgg cctgaccgga acccagcaag
     4081 aggcgctggc gcacgcgacg cagattgccg tgacggtgga gcccagcggc ggctcaccga
     4141 ccgggcagcc gagcggcacg ccggtattca tcgcgccgtt gcgcgcaatc tcctgagaga
     4201 ggggcagatg tgacgcggcg cgctgcccgt tacggggcag cgcgccgctt caaagcacgc
     4261 cggaacgcga tcagaaggac agataggagc cgacgttgaa gttgttgctc gtgttctcga
     4321 agtcgttgtg cgccagcgtc ttgacgtggc tgatctcgcc gagcaaggtc aggttcttgg
     4381 tcagcgagta gtagatgccg cccgtgacct tgttgttgtt gtcgagcagg ttcggcgtat
     4441 tgaagccatc ggcggcactg ccgaagtcca gccggctgct gccgtagttt acccccagct
     4501 tgacgggacc catggtgtag gtcgcctgca cgagatagcc atcagacttg cgcgcgtgtc
     4561 cgagcccgtc gtccgccagc acaaacaatg cggtcgtgcc gatgccatcg cccgtgtagt
     4621 aatagccgaa cagcgacagg ccgcccacat cgagtttcgc gaagaagtcg atgccacggc
     4681 tggagaagtc accggctacg ccagacacgt cataattctg cttctgcacg atccccgctg
     4741 tcgagagata cagcttgtcg ctgcccccac cgatcggcag ggtcaagctc gccttggcgt
     4801 ggaagcccgg cgcactcttg gggttgggcg tgccgggctc ggtgagggag ttgaccgga
//
LOCUS       DASRHN010000429        11399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_652004, whole genome shotgun sequence.
ACCESSION   DASRHN010000429 DASRHN010000000
VERSION     DASRHN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11399
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652004"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /gene="lptD"
                     /locus_tag="VFB37_17345"
     CDS             <1..700
                     /gene="lptD"
                     /locus_tag="VFB37_17345"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HZO24261.1"
                     /translation="FRNNRYVGADRVGDANQVSLGITSRLLNASDGKQFLSATFGQIY
                     YFTNPRVVIPGEEVRTDNRSDFVAQIALTAFQDWSADMGLQWDPQTERSERSQVNIQY
                     KPSSQSVINVGYRYQRDVLEQAEISGAWPISHSWNVYVRGVYSIKDGEALERFAGFEY
                     RACCWRVRFGARRFISTHPVAPATTGSQDTGIWLQLELAGLASVGSASDAFLTEAIRG
                     YTPLESASQKIRSP"
     gene            726..2060
                     /locus_tag="VFB37_17350"
     CDS             726..2060
                     /locus_tag="VFB37_17350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO24262.1"
                     /translation="MQRSRSLTRLAAVLALGAQVLAFQGQGVVQAQTRELATRGVLLD
                     RVVAVVNDGVVLDSELNDQVASVTERLRAQKLELPPENVLRQQVLERLVLQEIQSQRA
                     AHAGLKVPDDALNNALTEVAQRNNIPLSQLPEALAQQGIDYASYREEMRKELTLGLLR
                     QRDVLQRISITPREIDQFLAKQANAPSEQNEYNVSHILIAVGQTATPEQIEQASKRAE
                     EVYQRAKGGEDFAKLAIAYSNSQTALDGGALGWRKGTELPTFLTDIIVKLKPGEVSQP
                     LRTPSGYHLVKVNDIRNEEKKAIVDQVHVRHILMKTNELADDATVRQKLEQIRQRILK
                     GEDFAGIASITSEDPGSASQGGDLGWTAPGTFVPEFEAVISKLKDNELSEPFHTQYGW
                     HIAQVLGHRQVDNTDEIKRHQALEAIRASKADEETELWLRRLRDEAYVETKL"
     gene            2082..3065
                     /gene="pdxA"
                     /locus_tag="VFB37_17355"
     CDS             2082..3065
                     /gene="pdxA"
                     /locus_tag="VFB37_17355"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZO24263.1"
                     /translation="MQPARIVLTSGEPAGIGPELCLALARRRLPCELVCVGDRELLAA
                     RSAQLGWPVKLQPYDPAASHAHEPDTLVVIHHPLAAISHPGRLDKRNSRYVLDLLDRA
                     VEGTLRGEFAAMSTAPVHKGIINDAGIAFTGHTEYLAERTRAARAVMLLAADSMRVAL
                     ATTHLPLKEVSGAITVESLCAITEILAADLHKWWGMQSPRIGMCGLNPHAGEGGYLGD
                     EEIRVIAPAIERLRARGIQVTGPLPADTAFVPAVLAGFDAVIAMYHDQGLPVIKHAGF
                     DRTVNVTLGLPILRTSVDHGTALDLAGTGRADPGSLVAAITLAIELSSRSR"
     gene            3065..3898
                     /gene="rsmA"
                     /locus_tag="VFB37_17360"
     CDS             3065..3898
                     /gene="rsmA"
                     /locus_tag="VFB37_17360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZO24264.1"
                     /translation="MPESPRCGHPPRRKRFGQHFLHDPAVISRIVQAVSPAPADHLVE
                     IGAGRGTLTRSLLQTRCSTLDAIEIDRDLLALLHHELAHERRLTLHAGDALRFDFGAL
                     SRERSGPLRIVGNLPYNISTPLLFHLLKHRGAISDLHIMLQREVVARIAARPGGPDYG
                     RLTVMLSPWLRAEPLFEVGPGAFQPPPAVWSAVARLTVHPEPAFAVSPHFGAVVAAAF
                     AHRRKTLRNALRGLVSAGQIEASGLDPGARPGTLPPEAFNALAQKIVRSDPGAEADRA
                     E"
     gene            3895..4347
                     /locus_tag="VFB37_17365"
     CDS             3895..4347
                     /locus_tag="VFB37_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZO24265.1"
                     /translation="MKGHDMSNYRRILLVVDLTEDSVMIGRRAQALAAAVGAEVELLH
                     VVEFIPVEPMGETLMPSVQIEDDLLDQARQRLAVLGTDLGVPAAACRVESGNVKSEIV
                     RIARERHVDLIVLGSRERHGLSILVNFTEDTILHAAPCDVLAVRVGRP"
     gene            4361..4741
                     /gene="apaG"
                     /locus_tag="VFB37_17370"
     CDS             4361..4741
                     /gene="apaG"
                     /locus_tag="VFB37_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HZO24266.1"
                     /translation="MDSSSPPIRIQVQTAYLVEQSDPSDHRYVFSYTITIRNEGEVPA
                     KLLRRHWVITDANGKVQEVRGDGVVGEQPHLKPGQGFRYSSGAVLETPVGVMQGSYQM
                     MTDEGTRFDAPIAAFRLAMPGILQ"
     gene            4738..5577
                     /locus_tag="VFB37_17375"
     CDS             4738..5577
                     /locus_tag="VFB37_17375"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HZO24267.1"
                     /translation="MTRYALGDVQGCHDELLALLERVGFSADRDQLWFVGDLVNRGPR
                     SLDVLRYVRSLGDNAVVVLGNHDLHLLAVAYGSRRKRRSDTLDDILAAPDRDALLDWL
                     ITRPLAHVEAPRAGGECDLMVHAGVVPQWSVGKTVELAVEVAASLRRDPNSVFQHMYG
                     DEPDRWTEELSGPERLRFTINVLTRLRVCTAQGRVDLKLKGKPPAADSPFRPWFDLEG
                     RLTRSARVVFGHWSALGFIQRAGVVGLDSGCVWGGGLTAFDLDAERPPVTLPCREYQS
                     PGE"
     gene            complement(5606..6076)
                     /locus_tag="VFB37_17380"
     CDS             complement(5606..6076)
                     /locus_tag="VFB37_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24268.1"
                     /translation="MTRSDRPAITAILAGLALTWAAGCSHLPRPHWPWHHQAAQGPQE
                     VHELDIIAAEGSSVNLPQYWKQNTLVVDLQQAGATGSLVMKPREHTLWPVRIALRVMP
                     GQFAALEVRAKQRVVLPITPSGTRPVDLELAPGVYVMKTPEIHVSWGPELAVTQ"
     gene            6227..6739
                     /locus_tag="VFB37_17385"
     CDS             6227..6739
                     /locus_tag="VFB37_17385"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HZO24269.1"
                     /translation="MQPLQAFNLRRWIDENRAELRPPVGNKRVFRDGDFVIMVVGGPN
                     ARKDYHVDPGEEFFYQLEGDMVLRTMQEGRPVDLPVREGEILLLPPLVPHSPQRPGNT
                     VGLVVERRRRPGELEGFQWYCERCGQLLHEEFLEVTDIEKLAPVFERFYGSQEQRTCS
                     NCGATMERAD"
     gene            6736..7554
                     /locus_tag="VFB37_17390"
     CDS             6736..7554
                     /locus_tag="VFB37_17390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO24270.1"
                     /translation="MRARIELEHTALNVDLSRPVSIAIDLDFSAQPRHFGAPVALTRP
                     WSVPGFSGSVAQGASCNCHMLTLIPHCNGTHTESVGHLTTDPLDAFRIVPPGLVPALL
                     LSVEPVDVQDGESVDPPSRAGDLRITRRALLKSWPRNLPFEPNGLILRTLPNGADKKQ
                     RDYTELTAAYLTSEAAQCLVERGVQHLVVDLPSIDRTHDEGRLTAHRIFFGLPPGSRA
                     LSEATRSRATITEFAYIPNGLADGAYLLEIQTPAIAGDAVPSRPVLYALESLNP"
     gene            7551..8831
                     /gene="kynU"
                     /locus_tag="VFB37_17395"
     CDS             7551..8831
                     /gene="kynU"
                     /locus_tag="VFB37_17395"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZO24271.1"
                     /translation="MSHEPTLEYACALDEQDPLRSFRGQFALPADASGHPLLYLCGHS
                     LGLMPLEARRVVEEELDRWARLGVLGHEAESRPWIPYHRNVTAGLAYLGGARPDEVIA
                     MNSLTVNLHLMLASFYRPLGRRVKILIEAGAFPSDRHAVASQLGWHGHDPDRTLIELA
                     PARGEDTVSHEAIEACLDEHGADIALVLWPGVQYRTGQAFDVERVVQAAHRAGCIAGI
                     DLAHAIGNVPLELHAWGVDFAVWCNYKYLNAGPGAIGGCFVHERHLQSLPTTRLQGWW
                     GHEASTRFRMEPQFEPEPGAAAWQISNPPILSTAPLLPSLDLFRQAGIERLRGKSRSL
                     TGLLEFLLAPLGPDVALITPCEPAARGCQLSLRIAGRDRRGRRILDRLAQLGAVCDWR
                     EPDTIRVAPVPLYNTHADVFRFSELLAQALREPA"
     gene            8877..10205
                     /locus_tag="VFB37_17400"
     CDS             8877..10205
                     /locus_tag="VFB37_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO24272.1"
                     /translation="MNVVGAGLAGALLGITLAHRGCSVALYERRSDPRAAQTERGRSI
                     NLALASRGIRALEHAGVMHRIRPLLIPMRGRMIHERNGRQTLQPYGQRPEEVIYSISR
                     AALNRVLIEEAAAHPAVTIRFLHVCLGADSAAGRLRLRDEASQREYTVGLAPSIAADG
                     AGSAVRSSLAASGQVRVGEEWLDHDYKELTLPALGGRPALDPHALHIWPRGGFMLIAL
                     PNTDGSFTGTLFLAQRGPRSFAALSDPQSVLELFREEFADALALMPDLAGEFQQHPAG
                     QLGTVHALPWHVGAEVLLLGDAAHAIVPFHGQGMNAAFEDCLALDTLWEQCADWQTLF
                     AEFETLRRPNAEAIGRMALENYTEMRDTVLDPDFVRRQAIALELERRFPDRFIPRYSM
                     VSFHPEISYAEALRRGEVQTGILLEVDRGSAAPDFALAQKLVQERLPLLR"
     gene            complement(10234..10845)
                     /locus_tag="VFB37_17405"
     CDS             complement(10234..10845)
                     /locus_tag="VFB37_17405"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZO24273.1"
                     /translation="MAAPSVRPDAKSQHPGSGVLPDALQGSDRAQHRGGDLKALISLV
                     VAMAGNGVIGRDNTLPWYLPADLKHFKTVTLGKPILMGRRTFESIGRALPGRTNLVLT
                     RNPAWRAEGVIKVHSLAEAFERIEGAAELAGIGGTEIFRMLLPLAVRIYLTRIHADIP
                     GDTVFPPLDPAQWVESDARFVAADDRNAYSMTFATLDRIGSPG"
     gene            complement(10734..>11399)
                     /locus_tag="VFB37_17410"
     CDS             complement(10734..>11399)
                     /locus_tag="VFB37_17410"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZO24274.1"
                     /translation="VFVPLTAMDSVFVAGNMTKVSTGGWIPLTLGALLFFVFWTWRSG
                     RLELRAALAKMAVPKQDLAKVIDGAQRVPGTGVFLASDRDLVPSALIRNIEHNRVVHE
                     RVIILNIEIAHTPRQDPVRRVEIEEILPGVYEITARFGFMETPDVSQALKSCRTRGLR
                     VFSEDSSFFVGRHMVRARPLPGWKGLQRRLFARMQSHSTQAAEFFRMPFRGVIVLNTA
                     VEI"
BASE COUNT         1925 a         3763 c         3683 g         2028 t
ORIGIN      
        1 gtttcgcaac aaccgctacg tgggggcgga tcgggtcggg gatgcaaacc aggtgagcct
       61 cggcatcacc agtcggctgc tgaatgcgag cgacggcaaa cagttcctct cggccacctt
      121 cggccagatc tactacttca ccaatccgcg cgtggtgatt cccggggagg aggtgcgcac
      181 cgacaaccgc tccgacttcg tggcccagat cgccctgacc gctttccagg actggagcgc
      241 ggatatgggg ttgcagtggg acccgcagac cgagcgcagc gagcgctccc aggtgaacat
      301 ccagtacaag ccctcgtcgc agtcggtcat caatgtcggc taccgctatc agcgtgatgt
      361 cctggagcag gcggagatct ccggcgcgtg gcccatcagc cattcctgga atgtctacgt
      421 gaggggggtt tactcgatca aggacggcga ggcactggag cgtttcgcgg gcttcgagta
      481 ccgtgcctgc tgctggcggg tccgtttcgg ggcccgccgc ttcatcagca cgcatccggt
      541 ggctccggcc accaccggct cccaggacac cggcatctgg ctgcaactgg aactcgcggg
      601 cctggccagt gtcggatccg cgtccgatgc cttcttgacg gaagccatcc ggggctatac
      661 acccctggaa agtgccagcc agaaaatccg gagcccttga gctccccggc agacaaggat
      721 catccatgca gcgttctcgt tctctgaccc ggcttgcagc cgtgctcgcc ctgggcgccc
      781 aggtcctggc ttttcagggc cagggagtcg tccaggccca gacgcgtgag ctggccaccc
      841 gtggggtatt gctggatcgg gtcgtggcgg tcgtcaatga cggggtggtg ctcgacagcg
      901 agctgaatga tcaggtcgcc tcggtcaccg aacgcctgcg cgcgcaaaag ctcgagctgc
      961 cgcccgaaaa cgtcctccgg cagcaggtcc tggaacgcct ggtcctgcag gagatccagt
     1021 ctcagcgcgc cgcccacgcg ggcctcaagg tacccgacga tgccctgaac aatgcgctga
     1081 ccgaagtcgc ccagcgcaac aacattccct tgtcccagct ccccgaggcg ctggcccagc
     1141 agggcatcga ttacgcgtcc taccgtgagg agatgcgcaa ggagctcacc ttgggcctgc
     1201 tccgacagcg tgacgttctg cagcgaatca gcatcacgcc tcgtgagatc gaccagttcc
     1261 tcgccaagca agccaatgcg ccctcggagc agaacgaata caacgtgtca cacatcctca
     1321 ttgcggtcgg tcaaaccgcg accccggagc agatcgagca ggcctccaag cgggctgagg
     1381 aggtctacca gcgggccaag gggggtgagg acttcgcgaa gctcgccatc gcctactcca
     1441 acagtcagac ggcgctcgat ggcggggcgc tcggctggcg caaggggacc gagctgccga
     1501 ctttcctcac cgacatcatt gtgaagctca agcctggtga ggtgagtcag ccgctccgca
     1561 ctccgagcgg ctatcacctg gtcaaggtca acgacattcg caacgaggaa aagaaagcca
     1621 tcgtcgatca ggtgcacgtc cggcatatcc tgatgaagac caacgagctg gcggatgatg
     1681 ccaccgtgcg ccagaagctc gagcagatcc gtcagcggat cctcaagggt gaggatttcg
     1741 ccggcatcgc ctcgatcacc tcggaggatc ctggctcggc ttcccagggc ggggacctcg
     1801 gctggaccgc tccgggcacc ttcgtgccgg aattcgaggc ggtcatctcc aagctcaagg
     1861 acaacgagct cagcgagcct tttcatacgc agtacggctg gcacatcgcc caggtgctgg
     1921 gccaccgcca ggtcgacaac accgacgaga tcaagcgcca ccaggctctc gaggcgatcc
     1981 gcgcgagcaa ggcggatgag gagaccgagc tctggctgcg ccggctgcgc gatgaggcct
     2041 acgtagagac gaagctctag caggtcgcgc gcggaggacg attgcaacct gcgcgcatcg
     2101 tactgacttc gggcgagccc gccgggatcg gccccgagct gtgtctcgcc ctggcacgac
     2161 gccgcctgcc gtgcgagctg gtgtgtgtcg gcgaccgcga gctgctcgct gcgagatccg
     2221 cacagctcgg ctggcccgtg aaactccaac catatgatcc ggctgcctcg catgcgcacg
     2281 agccggatac gctggtcgtc attcatcacc cgcttgccgc gatcagtcat ccgggtcggc
     2341 tcgacaagcg caattcccgt tatgtgctcg accttctcga tcgtgcggtg gaagggaccc
     2401 tgcgcggtga gtttgccgcc atgagcacgg cgcctgttca caagggcatc atcaatgatg
     2461 cggggattgc ctttaccggg cacaccgagt atctcgccga gcgcacccgg gctgcgcgcg
     2521 ccgtcatgtt gcttgcggcg gacagcatgc gagtcgcact tgccaccact catctgccac
     2581 tgaaggaagt cagcggcgcc atcacggtgg agtcgctctg cgcgatcacc gagatcctcg
     2641 ccgcggactt gcataaatgg tggggaatgc agtcgccgcg catcgggatg tgcggcctca
     2701 atccgcacgc gggcgaaggc ggttacctgg gtgatgagga gatccgggtc attgcgccgg
     2761 caatcgagcg cctgcgcgcc cgcggaatcc aggtgacggg gccgctgccc gccgacaccg
     2821 cgttcgtgcc ggccgttctg gccgggttcg atgccgtgat cgccatgtac catgaccagg
     2881 gtttgccggt catcaagcac gcgggattcg atcggacagt gaacgtgaca ttggggctgc
     2941 cgatcctgcg cacctcggta gatcacggca ccgcactcga ccttgccggc accggacgcg
     3001 ccgacccggg cagcctggtc gcggccatca cactggcgat cgagctcagc agccgatccc
     3061 gctgatgccc gaatccccac gttgcggaca cccgcctcgt cgcaaacgct tcggacagca
     3121 tttcctgcac gatcctgcgg tgatcagtcg catcgtccag gcagtgagcc cggctccagc
     3181 ggatcacctg gtggagatcg gtgccggacg cggcaccctc acgcgaagcc tgctgcaaac
     3241 aaggtgcagc acgctcgatg ccatcgagat cgatcgcgac ctcctggccc tgctgcacca
     3301 tgagctggcc catgagaggc gactgacact gcatgcgggg gatgcgctgc gtttcgattt
     3361 cggcgcgctc tcacgcgagc gcagcggccc tctgcgcatc gtgggcaacc tgccctacaa
     3421 catttccact cccttgctgt ttcacctgct gaagcaccgc ggcgcaatct ccgacctcca
     3481 catcatgctt caacgcgagg tggtggcgcg catcgcagcg cgcccgggcg ggccggacta
     3541 cggccgcctg accgtcatgc tctcaccctg gctgcgggcg gagcctctgt tcgaggtcgg
     3601 tccgggagcc ttccaaccgc cgcccgccgt ctggtcggcc gtcgccagac tcacggtgca
     3661 tcccgagccg gccttcgcag tgagcccgca cttcggagcc gtcgtggcgg ccgcatttgc
     3721 ccaccggcgc aagacgctgc gcaatgcgct ccgcggtctg gtcagcgccg ggcagatcga
     3781 agcgagcgga ctcgacccgg gtgcccgccc cggcacgctc cctcccgagg cattcaatgc
     3841 gctagcgcag aagattgtaa gatctgaccc gggggccgaa gccgaccgcg ctgaatgaag
     3901 ggtcacgaca tgtccaacta tcgccgcatc ctgctcgtgg tcgacctcac cgaggacagt
     3961 gtgatgattg gccggcgcgc gcaggcgctc gccgccgcgg tcggcgccga ggtggagctg
     4021 ctgcacgttg tggaattcat accggtggaa ccgatgggcg agaccctcat gccctccgtg
     4081 cagatcgagg atgatctgtt ggatcaggcc aggcagcggc tcgcggtgct cggcaccgat
     4141 ctgggcgtcc cggccgcggc ctgccgggtc gagtccggta acgtcaaatc ggaaatcgtg
     4201 cggatcgcgc gcgagcggca cgtggatctc atcgtgctcg gcagtcgcga gcgccatggt
     4261 ctgtcgattc tggtgaactt cacggaagat acgatactgc acgccgcgcc ctgcgatgtg
     4321 cttgcggtgc gcgtaggccg gccctgatcg gcgcagcgac atggattcca gctcaccgcc
     4381 gattcgtatc caggtgcaga cggcctacct cgtcgagcag tccgatccca gtgaccaccg
     4441 ctacgtcttt tcctacacca tcacgatccg caatgagggc gaggtgccgg ccaagctgct
     4501 gaggcgccac tgggtcatca cggatgcgaa cggcaaggtg caggaggtgc gcggcgacgg
     4561 cgtggtcggc gagcagccgc acctgaaacc gggacagggg tttcgctact ccagcggcgc
     4621 cgtgctggaa acgccggtcg gcgtcatgca gggcagctac cagatgatga ccgacgaagg
     4681 cacgcgattc gatgcgccca tcgccgcctt ccgtctggcc atgcccggta tcctgcagtg
     4741 actcgctatg ccctcggcga cgtccagggc tgtcatgatg agttgctcgc gctgctcgag
     4801 cgagtgggtt tctccgcgga ccgggatcag ctctggttcg tcggcgacct ggtgaatcgc
     4861 ggaccgcgct cgctggatgt gctgcgctac gtacgcagcc tcggcgacaa cgcagtcgtc
     4921 gtccttggca accacgatct acatctgctc gccgtcgcgt acgggagccg ccgcaagcgc
     4981 cgctccgaca cgctggacga tattctggca gccccggatc gagacgcgct gctcgactgg
     5041 ctcatcacgc gaccccttgc tcacgtcgag gcgcctcgag cgggcggcga atgcgatctc
     5101 atggtgcatg ccggagtggt cccgcagtgg agcgtcggca agacggtcga gctggccgtc
     5161 gaggtggcgg cgagcttgcg ccgcgatccg aactcagtct tccagcacat gtacggcgat
     5221 gagcccgatc gctggaccga ggagctgagc ggtccagagc ggctgcgatt cacgatcaac
     5281 gtgctgacgc gtctacgcgt ttgtacggca cagggtcgcg tcgacctcaa gctcaaaggc
     5341 aagcccccgg cggcggattc gccttttcgg ccgtggtttg acctggaggg acgtctcacg
     5401 cgttccgcgc gcgtcgtatt cggtcactgg tcggcgctgg gtttcatcca gcgtgccggt
     5461 gtcgtggggc ttgattcagg ttgtgtctgg ggcggcggcc ttacggcgtt cgatctcgat
     5521 gcggagcggc cgccggtgac gttgccgtgc cgcgagtatc agtcccccgg cgagtgagcc
     5581 gcggaccttc gcgaagagtt cacgctcact gcgttacggc cagctcaggg ccccagctga
     5641 catggatctc gggcgtcttc atgacgtaca cgcccggagc cagctccaga tccaccggcc
     5701 gggttcccga aggggtgatc ggcaacacca cccgctgctt ggcacggacc tccagcgctg
     5761 cgaattgtcc cggcatcacg cgcagggcaa tacgcaccgg ccacaaggta tgttcgcgcg
     5821 gcttcatgac gaggctgccc gttgcgcccg cctgctgcag atcgacgacc agcgtgttct
     5881 gcttccaata ttgcggcaga ttcaccgatg agccctctgc agcaatgatg tccagctcgt
     5941 gcacttcctg cgggccctgc gcggcctggt ggtgccaggg ccagtgaggc cgcggaaggt
     6001 ggctgcagcc ggccgcccat gtcagcgcca ggccagcgag gatggcggtg attgccggac
     6061 ggtcggatcg ggtcatggac agcgctcctc gggcagctcc gccagcagcc ggcggaacat
     6121 ggcgcatctt agcgccacgc ccgcgctgtc actcgcggaa agctcaattt cgtgaggcgc
     6181 gttttcggcg cgtaacatag gggtgtagcc aatcgaggac acgggcatgc aacctctcca
     6241 ggcgttcaac ctgcgccgct ggatcgacga aaaccgcgcc gagctgcgac ctcccgtggg
     6301 caacaagcgc gtgttccggg acggtgattt cgtgatcatg gtggtcggag gtcccaacgc
     6361 ccgcaaggat tatcacgtgg atccgggcga ggaattcttc taccagctcg agggcgacat
     6421 ggtgctcagg acgatgcagg aggggcgccc ggtggatctg cccgtgcggg agggcgagat
     6481 cctgctgttg ccgccattgg taccgcattc accgcagcgg cccggcaata ccgtcggcct
     6541 cgtggtcgag cgccgccggc gtcccggtga gctcgagggc tttcaatggt actgcgagcg
     6601 ttgtggccag cttctgcacg aggagtttct cgaggtcacg gacatcgaga agcttgcgcc
     6661 cgtgttcgag cgcttctacg gcagtcagga gcagcgcacc tgctcgaact gcggcgccac
     6721 gatggagcgc gccgattgag ggcgcgcatc gagctggagc acacggcgct caacgtggac
     6781 ctgtcacgcc ccgtgagcat cgcgatcgac ctggattttt cggcgcagcc gcgccacttc
     6841 ggcgccccgg tggccctcac gcgcccctgg tcggtgcccg gcttctccgg ctctgtggcg
     6901 caaggcgcga gctgcaactg ccacatgctg accctgatcc cacactgcaa cggaacgcat
     6961 accgagtcgg tcggtcatct gacgaccgat ccgctggatg cttttcggat cgtccctcca
     7021 ggtctcgtac cagcgctgct tctcagtgtc gagccggtcg acgtccagga tggcgaatca
     7081 gtagacccgc cctcgagagc cggcgatctg cgcatcaccc gccgtgccct gctcaagagc
     7141 tggcctcgga acctgccgtt cgagcccaac ggcctgattc tgcggacgct gcccaatggt
     7201 gcggacaaga agcagcgcga ctacacggag ctgaccgccg cctacctgac aagtgaggcc
     7261 gctcaatgcc tggtcgagcg cggcgtgcag catctggtcg tggatctgcc ctccatcgac
     7321 cgcactcatg atgagggaag gctcacggcg catcgcattt tcttcggtct gccgccgggc
     7381 tcacgtgcgc tgtcggaagc aacgcgctct cgagcaacga tcaccgagtt tgcgtacatt
     7441 ccgaacgggc tggcggacgg ggcgtatttg ttggaaatcc agacgccggc catagcgggt
     7501 gatgcggtac cgagccgccc ggtactctat gcgctcgaga gtctcaaccc atgagccacg
     7561 agccgacact cgagtacgcc tgcgctctcg atgagcaaga cccgttgcgc agcttccgtg
     7621 ggcagttcgc attgcctgcc gatgcctccg gccatcctct gctgtacctg tgcggccact
     7681 ctctcggatt gatgcccctg gaagcgcgcc gcgtggtcga ggaggagctc gatcgctggg
     7741 ctcgtttggg agtgctcggt cacgaagcgg agtcccggcc gtggatcccg tatcaccgca
     7801 atgtgacggc ggggcttgcg tacctgggcg gtgcgcggcc cgatgaagtg atcgccatga
     7861 actctctgac cgtgaatctg cacttgatgc tggcgagctt ctatcgtccg ctgggacgac
     7921 gcgtgaagat tctcatcgag gcgggggctt ttccatccga tcggcacgcg gtcgcttcac
     7981 agctgggttg gcatggtcac gatcccgatc gcacgctcat cgagctggcg cctgcgcgcg
     8041 gcgaagacac cgtctcacat gaggcgatcg aggcgtgcct ggatgagcac ggcgcggaca
     8101 tcgcgctcgt cctctggccc ggtgttcaat atcgtacggg ccaggctttc gatgtcgagc
     8161 gagtcgtaca ggccgcccat cgcgccggct gcattgccgg catagacctg gcccacgcga
     8221 tcggaaacgt gccgctcgaa ctgcacgcct ggggcgtcga tttcgcagtc tggtgcaact
     8281 ataagtatct gaacgccggc cccggagcca tcggcggctg cttcgtccac gaacgccact
     8341 tgcagagcct gccgaccacc cgcttgcagg gttggtgggg ccatgaggcc agcacacgtt
     8401 tccggatgga gccgcagttc gagcccgagc cgggcgcggc cgcctggcag atcagcaatc
     8461 ctcccatcct gtcgacggcg ccgctgttgc cctcgctgga tctgttccgg caggcaggca
     8521 tcgaacggtt gcgtgggaag tcccgctccc ttacgggctt gctggaattt ctgctcgccc
     8581 cactcggccc cgatgtcgct ctcatcactc catgcgagcc tgccgcacgc ggctgccagc
     8641 tctcgctccg gatcgccggc cgggatcgcc gcggccggcg catcctggat cggctcgcac
     8701 agctcggcgc ggtctgtgac tggcgcgagc ccgatacaat ccgcgttgca ccggttccgc
     8761 tgtacaacac tcatgccgat gtttttcgtt tcagcgagct gctcgcccaa gccctgcgcg
     8821 agcctgcctg accggacctg acggcacctg ctgtgcgaat catttcggac ccaaccgtga
     8881 acgtcgtcgg cgccggcctg gccggcgcgc tgctcggcat cacgctggct catcgcggct
     8941 gctccgtcgc tttatacgag cggcgcagcg atccgcgcgc ggcgcagacc gagcgtggcc
     9001 gctccatcaa tctcgccctc gcctcccgcg gcatccgtgc gctcgagcac gcgggagtca
     9061 tgcaccgcat ccggccgctg ttgataccga tgcgcggacg gatgattcac gagcgcaacg
     9121 ggcgccagac tctgcagccc tacggacagc ggcctgagga ggtcatctat tcgatcagcc
     9181 gggctgccct caaccgggtg ttgatcgagg aggctgcggc acatcctgcg gttaccattc
     9241 gtttcctgca cgtctgcctc ggcgccgact ctgcggcagg tcgcctgcga ttgcgcgacg
     9301 aagcctcgca gcgtgagtac acagtcgggc tcgcgccgag tatcgccgcc gacggcgccg
     9361 gctccgccgt gcgctccagt ctcgcggcct caggccaggt gcgggtcggc gaggaatggc
     9421 tggatcacga ctacaaggaa ctgacgctgc cagcgttggg tgggcgcccc gccctcgacc
     9481 ctcatgcgct gcacatctgg ccgcgcggcg ggttcatgct gatcgccttg cccaatacgg
     9541 atggcagttt caccggtact ctgtttctcg cccagcgcgg acctcgcagc tttgcagcgc
     9601 tcagtgaccc tcagtcggtc ctcgagctgt tccgcgagga gttcgcggac gccttggcgc
     9661 tgatgcccga tctggcagga gagtttcagc agcatccggc gggccaactg ggaacggtgc
     9721 acgcgctgcc ctggcatgtc ggtgcagagg tgctgctgct gggcgatgcg gcccatgcca
     9781 tcgtgccctt tcacgggcag gggatgaatg cagcattcga agattgcctg gcgctcgaca
     9841 cgctctggga gcaatgcgca gactggcaga ccctgttcgc agaattcgag actcttcgcc
     9901 gccccaacgc cgaggccatc ggccggatgg cgctggagaa ctacaccgag atgcgagaca
     9961 cggtgctcga tccggacttc gtgcgcaggc aggcgatcgc tctggagctg gagaggcgtt
    10021 tcccggatcg cttcattcca aggtattcga tggtctcctt ccacccggaa atctcgtacg
    10081 ccgaggctct gcgccgcggc gaggtgcaga cggggattct gctcgaggtg gaccgcggct
    10141 cagccgcgcc cgacttcgct ctggcgcaga agctggttca ggagcgcctg ccgctgctgc
    10201 ggtgaatgcg ggcagggtcg cgcgttcaga ttttcaacct ggagagccga tgcggtccag
    10261 ggtagcgaac gtcatggaat acgcgttgcg atcgtcggca gccacgaagc gcgcatcact
    10321 ctcgacccat tgagccggat cgagaggcgg aaataccgtg tcgccaggga tgtcggcgtg
    10381 tatgcgtgtc aggtagatcc ggactgccag cggcaggagc atccggaaga tctcggtccc
    10441 cccgattccc gccagctcgg cagcgccttc gatccgctcg aacgcttcgg caagtgagtg
    10501 aaccttgatg acgccttcgg cccgccacgc cgggttacgt gtcaggacca gattggtgcg
    10561 gcccgggagc gctctgccaa tcgactcgaa ggtgcgtcgg cccatgagaa tgggtttgcc
    10621 gagggtcact gtcttgaagt gtttcagatc cgccgggagg taccagggca aagtgttgtc
    10681 gcggccaatg acgccattgc cggccatcgc gacgaccagt gagatcagcg ccttcagatc
    10741 tccaccgcgg tgttgagcac gatcactccc ctgaagggca tccggaagaa ctccgctgcc
    10801 tgggtgctgt gactttgcat ccgggcgaac agacggcgct gcaatccttt ccagcccggc
    10861 agcgggcgcg cgcgaaccat gtgccgtccg acgaagaacg agctgtcctc ggagaacacg
    10921 cgcagcccgc gtgtgcggca cgacttcagc gcctggctga catccggcgt ctccatgaac
    10981 ccaaaacgcg ccgtgatctc gtacacgccg ggaaggatct cctcgatctc gacccggcgg
    11041 accgggtcct ggcgcggggt atgcgcgatc tcgatgttga ggatgatcac ccgctcgtgc
    11101 accacacggt tgtgctcgat gttgcgaatg agcgccgaag gcaccagatc gcgatcgctc
    11161 gcgaggaata ctccggtgcc cggcacgcgc tgcgcaccgt cgatcacttt cgcgaggtcc
    11221 tgcttgggca ctgccatctt cgccagcgcg gcgcgcaact ccaggcgacc gctgcgccat
    11281 gtccagaaga cgaagaacag gagcgctccg agtgtcagcg gaatccagcc accggtgctc
    11341 accttggtca tgttccccgc gacgaacacg gaatccatcg cggtgagcgg aacgaatac
//
LOCUS       DASRHN010000430        16474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_164317, whole genome shotgun sequence.
ACCESSION   DASRHN010000430 DASRHN010000000
VERSION     DASRHN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16474
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164317"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="kdsA"
                     /locus_tag="VFB37_17415"
     CDS             <1..639
                     /gene="kdsA"
                     /locus_tag="VFB37_17415"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR01362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZO24275.1"
                     /translation="GPGLASGLQVLEAVRRTLGVPVLTDVHEDTPLEEVAAVVDVLQT
                     PAFLCRQTNFILNAASQGKPVNIKKGQFLSPWEMANVVEKARSTGNSSIMVCERGFSF
                     GYNNLVSDMRSLAIMRDTGCPVVFDATHSVQLPGGQGSSSGGQREFIPVLARAAIASG
                     VAGLFMETHPDPAKALSDGPNAWPLDRMEPLLRTLKELDRVVKANPLEEALL"
     gene            636..1922
                     /gene="eno"
                     /locus_tag="VFB37_17420"
     CDS             636..1922
                     /gene="eno"
                     /locus_tag="VFB37_17420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZO24276.1"
                     /translation="MSRIVDIRGREIIDSRGNPTVEADVVLDSGAMGRAAVPSGASTG
                     TREAVELRDGDPKRYGGKGVLKAVEHVNSVLRRALLGYDARDQAGLDARIIELDGTES
                     KGRLGANALLAISLANAHAAAREAREPLYRYLAGDREPIMPVPMMNIINGGAHANNSL
                     DIQEFMILPVGAPSFREALRYGAEIFHALKKLLNGRGLSTAVGDEGGFAPDLGSNAQA
                     LEVILSAIDQAGYRAGRDVCLGLDVASSEFFKDGHYELESEQRRFSAAEFTQYLAGLA
                     DKYPIISIEDGMSESDWDGWGLLTRSLGQRIQLVGDDIFVTNTKILREGIERHVANAI
                     LIKPNQIGTLTETLAAIEMADKARYAAVISHRSGETEDSTIADIAVATAATQIKTGSL
                     SRSDRTAKYNQLLRIEAELGTVRYAGKSAFPVPLRS"
     gene            complement(1974..3014)
                     /locus_tag="VFB37_17425"
     CDS             complement(1974..3014)
                     /locus_tag="VFB37_17425"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HZO24277.1"
                     /translation="MGKLRAMKRARSSSTILALALVIAAAAAPDGSAAQSVPRAAEPA
                     EPAEPAEPAEEVVVQGKHEGPRMWRISKDNHVMWILGTISPLPKKMTWQSDSVEEVLQ
                     QTQEVLPAWPSIGVGANPFTALRLYFTWRRIQKSPDHMKLQAQLPPELYARFMALKAR
                     YAPRDSKLEELRPMLAGGRLLDEALGASGLTMHNEVQQTVLKLARKQGVRIHQTKLKV
                     DDPVDVLKDLGDTPKAGEIACLAAIVSRLETDMAPMQERARAWALGDVDTLRKLPHTQ
                     DDRIACLAAVSSSERIRNLVLRAEDDWLIEAEDTLRRNQATLAVQSMDRLLGDRGILA
                     TLSSKGYTVEGP"
     gene            3051..3371
                     /gene="ftsB"
                     /locus_tag="VFB37_17430"
     CDS             3051..3371
                     /gene="ftsB"
                     /locus_tag="VFB37_17430"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HZO24278.1"
                     /translation="MRWLAGALVVVVVLLQYRIWLSGDGLREVRRLESAVAGQRAENG
                     QLGERNRQLAAEVRDLKNGMAALEERARSDLGMIGENETFYQVVPPRPGSSGGESHTR
                     TAAR"
     gene            3404..4120
                     /gene="ispD"
                     /locus_tag="VFB37_17435"
     CDS             3404..4120
                     /gene="ispD"
                     /locus_tag="VFB37_17435"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZO24279.1"
                     /translation="MRYWLVMPGAGRGRRFGEALPKQYAELCGRTVIEWALTPFLTDS
                     RCAGAVVVIASDDAHWSKLAERVPRIGCAIGGTHRCESVRSGLAALEGRATQADWVLV
                     HDAARPCLAAQDLERLLERLQEHPVGGLLAVPAADTLKRTRGGHTVLETVDRSELWRA
                     LTPQMFRYGRLCKALDAAAAVGRVPTDEAQAIEWLGDAPLTVEGSSANIKVTTADDLL
                     IAAALLGARSIDCASGGQSR"
     gene            4117..4590
                     /gene="ispF"
                     /locus_tag="VFB37_17440"
     CDS             4117..4590
                     /gene="ispF"
                     /locus_tag="VFB37_17440"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZO24280.1"
                     /translation="MRVGSGIDVHAFGPGEFVMLGGVRVNHSRGVIAHSDGDVVLHAL
                     CDALLGAAGLGDIGQHFRDTDPRWRGADSRQFVQAVMQMLRARGLRVGNADITVLAEA
                     PKVGPVREQIREQLSQLLEIATEQVNVKATTTEHLGFLGRSEGIAAQAIVLLLDT"
     gene            4631..5713
                     /locus_tag="VFB37_17445"
     CDS             4631..5713
                     /locus_tag="VFB37_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HZO24281.1"
                     /translation="MLELWKQHALDPPRAHGAPLGCAILRRVAEDFLVEEDLGFAPAG
                     TGQHILLRIRKRNSNTQWVARELARACPCRPADVGYAGLKDRHAVAVQWFTVPRAARS
                     LDSWLALKTPEYEVLEAHAHTRKLPRGALAANRFEIRAREVTEEQGALAERMDRIARE
                     GVPNYFGPQRFGREGANLKRIAMPLHTLRPAERGFVLSAARSLIFNSVLAARVADQSW
                     GRLEPGDLANLEGRGSFFAIDSLDDLLRARCEHLDLHPTGPMWGAGEPGTSQRIGQLE
                     RQAAAALAPAADLVIGAGMRQERRSLRISVSDLRWSREGPDVSIGFRLGRGSFATTVL
                     RELFSLKVANREGATQAQFAEGSIEE"
     gene            complement(5714..6274)
                     /locus_tag="VFB37_17450"
     CDS             complement(5714..6274)
                     /locus_tag="VFB37_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZO24282.1"
                     /translation="MSHKPDEDEARLFREAVRDVKPLAREVSRPVIPVPGIPRPQPRA
                     RFARADRQAVLEESLYGEVADPELAGGEELVFRRPGVQPTIVRKLRRGYYRVQAELDL
                     HGLTVADAKQVLREFIASALEHHLRCVRIIHGKGLRSGHRGPVLKAAMAHVLRRTGHV
                     LAYVSARQVDGGTGAVYVLLAPWQRS"
     gene            6364..7122
                     /gene="surE"
                     /locus_tag="VFB37_17455"
     CDS             6364..7122
                     /gene="surE"
                     /locus_tag="VFB37_17455"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZO24283.1"
                     /translation="MRILVSNDDGFRSPGIRRLREALGTLAQVTVVAPDRNRSGASNS
                     LTLDVPLRVFEAEPGIYFVPGTPTDCVHLAISGLFDHAHDMVVSGVNDGPNLGDDVLY
                     SGTVAAAIEGRFLGLSAIAVSLCTAPESERHFEAAAEVARRLVAQLLRRPLERPLILN
                     VNVPDQPYAQLRGFRTTRLGFRHPSEAIVRAEDPHGRPVYWVGPAGPQQEAGPGTDFH
                     AVAEGYVSVTPLQIDLTRHAALPALASWLEGIDD"
     gene            7115..7780
                     /locus_tag="VFB37_17460"
     CDS             7115..7780
                     /locus_tag="VFB37_17460"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZO24284.1"
                     /translation="MTDVRLTGIGMTSARTRDRLVQRLREQGIANLAVLDRIRNVPRH
                     IFVDEALGSRAYEDTALPIGFGQTISQPYIVARMTEALLEGGDLHSVLEVGTGCGYQT
                     TVLAPLVDRLYTIERIEPLLLRAKERLKELGIRNVRFRHGDGRVGWKSHAPYDGILVA
                     AAPLTVPEGLIKQLKVGGRLLVPIGPEGEQQLLRLTRKEQRVERESLGAVAFVPLLGG
                     TQQ"
     gene            complement(8294..8662)
                     /locus_tag="VFB37_17465"
     CDS             complement(8294..8662)
                     /locus_tag="VFB37_17465"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HZO24285.1"
                     /translation="MKFTLEGAASNLIRSYSSEEIRVGNQSVRSSCIVTADALIRDWR
                     PRTLDELEPSDLEAVFALHPELALLGTGRRHQFAPAAIRSAFAAHSISVESMDLGAAC
                     RTFNILVQEERRVAALLFLE"
     gene            8677..9816
                     /locus_tag="VFB37_17470"
     CDS             8677..9816
                     /locus_tag="VFB37_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24286.1"
                     /translation="MRKPHAVYDTPARVEFRAAGRSHTVHEIALVFPDLYFPRELERD
                     DSPALALPGLEHITRFGQRSGLPQGWRPWLANWAGSPELASLPPALVAARIAACSTTP
                     TSVAGASLPGGAVWMATPVHLVASLTSLHLDRRSVLYLADSEELALCADFARTFQGSG
                     YHLTPLEGGGLLLFGAATSAVETCEPARAIGENVAERLPSGPGAAALRKLGAEIEMWL
                     HGHPLNEARLRRGELGITSLWLWGGGESGELARLREWTPAASARARVAFGSDASLRAL
                     WAHIDAKVQPLPPQLAQILSYPAEGSAVLVIEAGRMLHSNQNWMLPEALAEIDRQFLA
                     PAVQALQHRQVGVLEVLANDWRVSVRATDRLRLWRRARPGLSGLR"
     gene            9837..11561
                     /gene="recJ"
                     /locus_tag="VFB37_17475"
     CDS             9837..11561
                     /gene="recJ"
                     /locus_tag="VFB37_17475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZO24287.1"
                     /translation="MRRSFGGDCAELGPQLHPVLQRIYAGRGVRSPTELDTSLERLLP
                     VGSLEGIAAAVELLLRHRSGGRILVVGDFDADGATSTALVIRALRCWGFESVDFLVPN
                     RFEFGYGLTPEIVALAAQRSPTLIVTVDNGITSLEGVAAARTLGISVLITDHHLPGAQ
                     LPQADAIVNPNLPGSHFGSRALAGVGVAFYVMAALQRRLDELQLTPASAARAAEFLDL
                     VALGTIADLVPLDPNNRVLVLQGLKRIRAGRCVPGITALLEIAARRRADLDASDLAFA
                     VAPRLNAAGRIDDMSIGIQCLLAGQPDAARQLAERLDALNVERRTIEARMQMDALAAV
                     RRLTDPGPKSIHRCGVCLYDDSWHQGVVGLVAGRMADRLRRPVVAFALAADAQLRGSA
                     RSVRGIHVRDVLDAIATREPGLIGRFGGHAMAAGLTLERGNLDRFARCFDEEVERWAG
                     NCPAADAVETDGELSVWEIALETAHALRAGGPWGQAFPEPCFDGLFAIRNARVVADRH
                     LKMWVEVPRTGRSFDAIAFNHIEEGSEFTPPQGDVQLVYRLDINEYQGERRLQLLVDH
                     VMSGNQGP"
     gene            11620..12738
                     /gene="prfB"
                     /locus_tag="VFB37_17480"
     CDS             11620..12738
                     /gene="prfB"
                     /locus_tag="VFB37_17480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZO24288.1"
                     /translation="MPPKPLCLWKSISSNVSSRISGSGSALFGGFFEYDQKKERLEEV
                     GRELEDPAVWSDQGRAQELGRERARLAAAVQEIDRADKGIAEAAELLELAESEGDEAA
                     ARDIETDAGGLEADVRRLELKRMFRHEMDSHNAFLDIQAGAGGTEAQDWAQMLLRMYL
                     RWGAAHGFSSEVIDSNPGEVAGIKSATVEIKGDYAYGWLRTETGVHRLVRKSPFDSGN
                     RRHTSFASVFVSPEVDEDIDIEINPADLKTDVYRSSGAGGQHVNKTESAVRITHLPSG
                     IVVACQNERSQHKNRSTAMKMLKAKLYELEVNKRNAAARVLEDSKSDVSWGNQIRSYV
                     LDQSRIKDLRTGVEVGNTTAVLDGDLDQFMEASLKAGV"
     gene            12759..14267
                     /gene="lysS"
                     /locus_tag="VFB37_17485"
     CDS             12759..14267
                     /gene="lysS"
                     /locus_tag="VFB37_17485"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZO24289.1"
                     /translation="MSEMDKAQDENKLIAERRTKLAALREGAAAGGAYPNDFRRDSLA
                     GQLHDAFGERDAAWLEANPTRVQVGGRMLLKRPHGKVSFAQIADRTGQIQLFLQHDAL
                     GPAYEAFKGWDVGDIVGASGVLFRTKTGELSVRADSLRLIVKSLRPLPGKWHGLADTE
                     TRYRRRYVDLIVNETSRNVFRVRTRIIRFLRDYLDALDFMEVETPMMQPIPGGAAARP
                     FVTHHNALDLEMYLRIAPELYLKRLIVGGLERVYEINRNFRNEGLSTQHNPEFTMLEL
                     YLAYADYTQLMDWVEKAIRGMADAVHGSRQMQYQGRQYDLAAPFRRVTVEQAIVGCNP
                     GIDPLSLRDLTYLRKLCEKLGVPTGAQDGAGKLQMEIFDKTVEHTLLDPTFVHAYPAE
                     VSPLSRANDGDPFVTDRFEFFLAGREIANGFSELNDPEEQAARFRAQVARKERGDEEA
                     MFYDADYVRALEYGMPPAAGLGVGIDRLVMFFTDSPSIRDVILFPHLRPEAD"
     gene            complement(14279..15211)
                     /locus_tag="VFB37_17490"
     CDS             complement(14279..15211)
                     /locus_tag="VFB37_17490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24290.1"
                     /translation="MQSRELIALDELGIVRMRGPDVIRFLQGQLSSDVSQLSAERSLL
                     GGYHNPQGRAIALLRLLQLDAQDILAIVPRDLAATLVSRLARYILRAKVQITAELESW
                     RVGGCIGELAAPAGPSLRSARDAQTRLGEGVLAQVGWGSPRWLLVCPAGAAAPELGFD
                     RWAERDGSQDWLRLDIAEGVPQISAATSEQFVAQMLNLDAIGAIAFDKGCYTGQEVIA
                     RAHYRGRVKRRMQRFLSRTPCRLAPGDAGQLADGRSFKVVLAASRPEGYCEFLAVAAL
                     PEGASATPDADAPQSSEAAALSVEALPLPYALPD"
     gene            15413..15808
                     /gene="sdhC"
                     /locus_tag="VFB37_17495"
     CDS             15413..15808
                     /gene="sdhC"
                     /locus_tag="VFB37_17495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZO24291.1"
                     /translation="MRARPLSPHLSVYRMSRYTLVTSILNRFTGAGLSVGLLLFVYWL
                     MAVASGGRAYAQAQHVLAHGIFKVLYAALVIAVCYHLVAGIRHLVWDTGHGLERAQSQ
                     RSAWLVIAVSLVLIALVGFCLLSARGQGA"
     gene            15805..16194
                     /gene="sdhD"
                     /locus_tag="VFB37_17500"
     CDS             15805..16194
                     /gene="sdhD"
                     /locus_tag="VFB37_17500"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZO24292.1"
                     /translation="MSSLRSPLGRVAGLGAARGGVQHWWLQRLTSIALVPLAVWFLVC
                     LLRLPAFDHATVTGWMSQTWTAVLLILLLLVACWHSQIGIRVIVEDYVHGAGARTLTL
                     VIVTFAHTFIAFAGVFAILKVAFGAAR"
     gene            16191..>16474
                     /locus_tag="VFB37_17505"
     CDS             16191..>16474
                     /locus_tag="VFB37_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZO24293.1"
                     /translation="MSTYEFIDHTYDVIIVGAGGAGLRATLGLAEAGLSTACISKLFP
                     TRSHTVAAQGGISAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEFMCKEA"
BASE COUNT         2729 a         5322 c         5526 g         2897 t
ORIGIN      
        1 ggcccgggcc tggcgtcggg gctgcaagtg ctcgaggccg tgcggcgcac gctcggggtg
       61 ccggtgctta ccgatgtcca cgaggacacg ccgctcgagg aggtcgcagc cgtcgtcgat
      121 gtcctgcaga cgcccgcctt cctgtgccgc cagaccaact tcatcctgaa tgccgcttcc
      181 cagggcaagc cggtcaacat caagaaaggg cagttcctct caccctggga aatggcgaac
      241 gtggtcgaga aggcgcgctc caccggcaac tcgagcatca tggtctgcga gcggggattt
      301 tccttcggtt ataacaacct ggtctccgat atgcgctcgc tcgcgatcat gcgcgacacc
      361 ggctgtccgg tggtcttcga tgcgacgcac tcggtccaac tgcccggcgg gcagggctcc
      421 tcctcaggcg gccagcgtga gttcatcccc gttctcgcgc gcgctgcgat cgcctccggc
      481 gtcgcgggcc tgttcatgga gactcacccg gatccggcca aagcgctctc cgatgggccc
      541 aatgcctggc cgctggatcg catggagccg ctgctgagga cactcaagga gctggatcgc
      601 gtggtcaagg ccaaccctct ggaggaagcg ctgctatgag ccgaattgtc gacatccgcg
      661 ggcgcgagat catcgattcg cgtggcaacc ctacggtcga ggcggacgta gtgctcgact
      721 ccggcgccat gggccgcgcc gccgtgccct ccggcgcttc gaccggtaca cgcgaggcgg
      781 tcgagctgcg cgacggggat cccaagcgct atggtggcaa gggcgtgctg aaggcggtcg
      841 agcacgtgaa cagcgtgctc cggcgcgcgc tgctcggcta tgacgcacgc gatcaggcgg
      901 gcctggatgc gcgcataatc gagctcgacg gcaccgagag caaggggcgg ctcggagcca
      961 acgcgctgct ggcgatctcc cttgccaacg ctcacgccgc ggcgcgcgag gcgcgggagc
     1021 ccctgtatcg ctatctcgcc ggggaccgcg agccgatcat gcccgtgccc atgatgaaca
     1081 tcatcaatgg cggtgcgcat gccaacaaca gcctggacat ccaggagttc atgatcctgc
     1141 cggtgggcgc accgagcttc cgcgaggcgc tgcgctacgg ggcggagatc ttccatgccc
     1201 tgaaaaagct gctcaacggc cgcggactgt ccaccgctgt cggagacgag ggcggcttcg
     1261 cgcccgatct cggctcgaac gcgcaagctc tcgaggtgat cctgagcgcc atcgaccagg
     1321 ccggctatcg ggccggtcgg gatgtctgcc tggggctcga tgtcgccagc tctgaattct
     1381 tcaaggacgg gcactacgag ctggagtccg agcagcgccg cttcagcgcc gccgagttca
     1441 cgcaatatct cgcaggactg gccgacaagt acccgatcat cagcatcgag gacggcatga
     1501 gcgagagcga ctgggacggc tggggcctgc tcacgcgctc gctcggtcag cgcatccagc
     1561 tcgtcggcga tgatatcttc gtcaccaaca cgaagatcct gcgcgaaggc atcgagcggc
     1621 acgtcgccaa cgccatcctg atcaagccga atcagatcgg gacgctcacc gagacactcg
     1681 ccgccatcga gatggcggac aaggcgcgct atgccgcggt gatctcgcat cgctccgggg
     1741 agaccgagga cagcaccata gccgacatcg cggtggccac cgcggccacg cagatcaaga
     1801 cgggctcact gtcgcgctcg gatcgcacgg ccaagtacaa ccagctgctg cgcatcgagg
     1861 ccgagctcgg cacggttcgc tacgccggaa aaagcgcctt tccagtgccc ctccggagct
     1921 gaggtccttc caccagacct ggaggctcag ggtccttcac cagacctgag ggctcagggt
     1981 ccttccaccg tgtagccctt cgagctcagg gtcgcgagga ttccccgatc gccgagcagg
     2041 cggtccatgg actgcacggc gagcgttgcc tggttgcggc gcagggtgtc ctccgcctcg
     2101 atcagccagt cgtcctcggc ccgcagcacc aggttgcgaa tgcgctccga gctcgagacc
     2161 gcagccaggc aggcgatgcg atcgtcctgg gtgtgcggca gcttgcgcag cgtgtccaca
     2221 tcccccagcg cccacgctcg ggcacgctcc tgcatgggag ccatgtcggt ctccagccgg
     2281 ctgacgatcg cggcgaggca ggcgatctcc cccgccttgg gcgtatcccc aaggtccttc
     2341 agtacgtcca cggggtcgtc caccttgagc ttggtttgat ggatgcgcac cccctgcttg
     2401 cgcgcaagct tcaacaccgt ttgctgcact tcgttgtgca tcgtcaggcc ggaggctccg
     2461 agggcttcat ccagaagtct tccgccggcc agcatgggcc gcaactcctc gagcttgcta
     2521 tcgcgcgggg catagcgcgc cttgagcgcc atgaagcgcg catacagctc aggaggaagc
     2581 tgggcctgca gcttcatgtg atccggactt ttttgaatgc ggcgccaggt gaagtacaac
     2641 cgcagtgccg tgaacgggtt ggcgcccacg ccgatggagg gccaggccgg cagcacttcc
     2701 tgtgtctgct gcaacacctc ctcgacggag tcggattgcc aggtcatctt cttcggcagc
     2761 ggtgagatcg tgcccaggat ccacatcacg tggttgtcct tgctgatgcg ccacatccgg
     2821 ggtccttcgt gcttgccctg cacgacaact tcttcggcgg gctcggcggg ctcggcgggc
     2881 tcggcgggct cggcggcccg cggcacgctc tgagcagccg agccgtccgg tgcagcggcg
     2941 gccgcgatca ccagcgcgag cgccaggatc gtgctgcttg aacgtgcccg cttcatcgcc
     3001 cgaagttttc ccatgatcta gtgtgcatca agtgataggc tatcgcgctc atgagatggc
     3061 tcgctggcgc gttggtcgtg gtcgtggtgc tcctgcagta ccggatatgg ctctccggcg
     3121 atggcctgcg cgaggtgcgc cggctcgaga gcgctgttgc gggccagcga gccgagaacg
     3181 gccagctcgg cgagcgcaat cgacagctgg cggccgaagt gcgcgatctg aagaacggca
     3241 tggccgcact cgaggagcgc gcgcggagcg atctgggcat gattggagag aacgaaacct
     3301 tctaccaggt tgttcccccg cggcccggtt cctccggcgg cgaatcgcac accaggaccg
     3361 ccgcacggta gcccgcatcg agcccgatcg gaccgagagc tccatgcgtt actggctcgt
     3421 catgcccggc gccggaaggg gccggcgatt cggcgaggcc ttgcccaaac aatacgccga
     3481 gctgtgcggc cggaccgtga tcgaatgggc gctcactccg tttctcaccg acagccgctg
     3541 tgcgggtgcc gtagtcgtca ttgcctcgga cgatgctcac tggagcaaac tcgccgagcg
     3601 ggtaccccgg attggctgcg caatcggcgg gacgcatcgc tgcgagtcag tgcgcagtgg
     3661 actggctgca ctcgaggggc gcgcgacgca ggccgattgg gttctggtgc acgatgcggc
     3721 gcggccttgt cttgcagcgc aggatctcga gcgcctgctc gagcgtcttc aagagcatcc
     3781 tgtcggcggg ctgctcgccg tgcccgcagc agacacgttg aagcggaccc gtggcgggca
     3841 tactgtgctc gaaacagtgg atcgcagtga attgtggcgt gccttgaccc cgcagatgtt
     3901 ccgttacggc agactctgca aggcgctcga tgcggcggcc gccgttggtc gagtgcccac
     3961 cgatgaggcc caggcgatcg agtggctcgg agatgcgcct ctcactgtgg aaggctcctc
     4021 ggccaacatc aaggtcacca cggccgatga tctgctgatt gctgccgcgc tgctcggcgc
     4081 gcgcagcatc gattgtgcct ctggaggaca gtcccggtga gagtcggctc cggcatcgat
     4141 gtccatgcct tcgggcccgg tgaattcgtc atgctgggcg gagtccgggt gaatcactcg
     4201 cgcggcgtga ttgctcactc ggatggcgat gtcgtgttgc atgcactttg cgatgcattg
     4261 ctgggcgcgg cgggactggg ggacatcggc cagcatttcc gcgataccga tccgcgctgg
     4321 cgcggcgctg acagccgcca gttcgtgcaa gcggtgatgc agatgctgag ggctcgcgga
     4381 ctgcgggtcg gcaatgccga tatcacggtg ctggcggaag ctccgaaggt cggacccgtg
     4441 cgcgagcaga tccgcgagca gctttcgcag ctgctcgaga ttgcgaccga gcaggtcaat
     4501 gtgaaggcga cgaccaccga acacctgggc tttctggggc ggagcgaggg catcgctgcg
     4561 caggcgatcg tccttcttct ggatacctga gtccggcacg ccctcgctca cccggcggcc
     4621 tcacatctcg atgctcgagc tctggaagca gcatgcgctc gatcccccac gggctcacgg
     4681 cgcaccgctc ggctgtgcaa tcctgcgccg ggtggctgag gacttcctcg tcgaggagga
     4741 ccttggcttc gccccggcgg gcaccggcca gcacatcctg ctgcgcatcc gcaagcgcaa
     4801 ttccaacacc cagtgggtcg cccgcgagct ggcgcgagcc tgtccctgca ggccggcgga
     4861 tgtgggctat gctgggctga aggatcgaca cgcggtggcc gtgcaatggt tcaccgtgcc
     4921 gcgcgccgca cgctcgctgg actcttggct cgcgctcaag accccggagt acgaggtcct
     4981 ggaggctcat gctcatacgc gcaagctgcc gcgcggcgcg ctcgccgcga accgcttcga
     5041 gatccgcgca cgcgaggtga ccgaagagca gggcgcgctg gctgagcgga tggatcggat
     5101 cgcgcgcgag ggagtgccca attacttcgg gccgcaacgc ttcggtcgtg agggcgccaa
     5161 cctcaagcgc atcgcgatgc cgctgcatac gttgcgacct gccgagcgtg gcttcgtgct
     5221 ctccgcggcc cgcagcctga tcttcaactc cgtgctcgcg gcgcgggtcg cagaccagag
     5281 ctgggggcgg ctcgagccgg gcgatctggc gaacctggag ggacgtggga gcttcttcgc
     5341 catcgacagc ctcgatgacc tgttgcgggc gcgatgcgag cacctcgatc ttcacccgac
     5401 cgggccgatg tggggtgcgg gcgagcccgg gacctcccag cgcatcggtc agctggaacg
     5461 ccaggcggcc gctgcgctcg ccccggcagc cgatctcgtc atcggcgccg gcatgcgaca
     5521 ggagcgacgc agtctgcgga tctcggtgag cgacctgcgc tggagccgtg agggcccgga
     5581 cgtctccatc ggatttcggc tcggtcgcgg tagtttcgcc acgaccgtgc tgcgcgagct
     5641 tttcagtcta aaggtcgcca accgcgaggg tgcaacgcaa gcacagttcg ctgagggctc
     5701 gatcgaggag tgatcaagac ctctgccagg gcgcgagcag cacgtacacc gcgcctgtgc
     5761 cgccatcgac ctggcgcgcc gagacataag cgagcacatg gccggtccgg cgcagcacgt
     5821 gcgccatggc ggccttcaga accgggcccc gatgccctga gcgcaggccc ttgccatgga
     5881 tgatcctgac acagcgcaag tggtgctcga gtgcgctggc gatgaattcg cgcagcacct
     5941 gcttcgcatc ggccaccgtg aggccatgca ggtcgagctc ggcctggact cggtaatagc
     6001 cacgacgcag cttacgcacg atggtcggct gcacgccggg cctgcgaaag acgagctcct
     6061 cgccgccagc taactccggg tcggcgactt ccccgtacag gctttcttcg agcacggcct
     6121 gccggtcggc acgcgcgaac cgcgcccgtg gctgcggacg tgggataccg gggacgggaa
     6181 tcacgggacg ggacacctcc cgcgcgagcg gcttgacgtc ccgaacggcc tcccggaaca
     6241 ggcgtgcctc gtcctcatca ggcttatggc tcacgtttcc cccgcagcca ctttcagcaa
     6301 tataaccagt atagtgacgc gcctttcagg cgctccagtc ccacgcatgc gcacgataac
     6361 cccgtgagaa tcctggtcag taacgacgac ggcttccgct cgccgggtat tcgccgcctg
     6421 cgcgaagcgc tcggcacgct ggcacaggtc accgtggtcg cgcccgaccg caatcgcagc
     6481 ggggcgagca actccctcac gctcgatgtc ccgcttcgcg tgttcgaggc ggagccgggc
     6541 atctacttcg ttccagggac gccgaccgat tgtgtccatc tggccatctc cggactgttc
     6601 gaccacgcgc acgacatggt ggtttcagga gtgaacgacg gaccgaacct cggggatgac
     6661 gtgctgtact ccggaacggt ggcagcggcg atcgaaggcc gtttcctggg cctgtccgcg
     6721 atcgcggtct cgctgtgcac cgccccggaa agcgagcggc atttcgaggc ggcggcggaa
     6781 gtggcgcggc gactggtcgc ccagctgctg cgcaggcctc tcgagcggcc cctcatcctc
     6841 aacgtcaacg tgcccgatca gccgtatgcc cagctgaggg gctttcgcac cactcgcctg
     6901 ggtttccgcc acccctccga ggcgatcgtg cgcgccgagg atccccatgg ccggcccgtc
     6961 tattgggtcg gtccggccgg gccccagcag gaggccggac ccgggacgga ctttcatgcg
     7021 gtcgcggaag ggtatgtctc ggtgacgccc ctgcagatcg atctgacacg tcacgcggcc
     7081 ttgcccgccc tggcgagctg gctggaaggc attgatgact gacgtccgtc tcaccggcat
     7141 cggcatgacc tctgcccgca cccgggaccg tctcgtccag cgcctcagag agcagggcat
     7201 agccaacctc gccgtactcg accgcattcg caacgtgccc cgccatatct tcgtggacga
     7261 ggccctcggc agccgcgcgt acgaggacac ggctctgccc atcggattcg gccagacgat
     7321 ctcgcagccc tacatcgttg cccgcatgac cgaagcgctc ctggaggggg gcgatctgca
     7381 cagcgtgctg gaggtgggga caggatgcgg ctaccagacc acggtactcg cccccctggt
     7441 ggatcgcctc tacacgatcg agcggatcga gccgctgctg ctgcgagcca aggagcgcct
     7501 caaggagctc ggcatccgca atgtccgctt ccgtcacggc gatggcaggg tcggctggaa
     7561 atcgcatgcc ccctacgatg ggatcctggt agccgccgcg ccgctgaccg tgccggaggg
     7621 gctgatcaag cagctcaagg tgggaggccg gttgctcgtg ccgatcggac ctgaaggcga
     7681 gcagcagctg ctgcgcctga cacgcaagga gcagcgggtc gagcgggagt cgctcggcgc
     7741 cgtggcgttc gtgccgctgc tcgggggtac ccagcaatga gggcgcccca acgaattact
     7801 tacattaatg aaacgatgta aggatctgat ccgacaggtt gttatgactc ggatagcagg
     7861 ctcgcgatgc tccctctgaa cgcgccgacg ccggatttga cccccaatca gctgcttcgg
     7921 agcccttccg gcggcgcgcc gaggcctcgc gatgaggtgg ggcagcgctc ggggctccca
     7981 gccagcgagg aaaaattcct gcggctccaa agagcttgcg cgcttatctg tgacccgttt
     8041 aacatcattc acctgtcgcc tgatgacgac agccctcgac ggcggactta tgaagacaca
     8101 gggaggaggg gagtcgagag aaaagcaaca agtcggcgag aagaaaaaca ataacgggca
     8161 attgttcgag tctgtcagct tcgaatgatt gtttggttgg ttgagagctt tctcccccct
     8221 gtgtcttctt aagccgccgt cagcggcgcc ccccgggggg cgcctgcgcg cacccaacct
     8281 cgagcgcacg ctctcactcc aggaacaaca aggccgcgac gcgccgctcc tcctgcacca
     8341 ggatgttgaa tgtgcggcag gcggcgccca ggtccatgga ctccacgctg atcgagtgcg
     8401 cagcgaaggc tgagcgaatt gctgcaggcg caaactgatg ccgccgcccg gtgccgagca
     8461 gcgccagctc ggggtgcagc gcaaagaccg cctcgagatc gctcggctcg agctcatcga
     8521 gcgtgcgggg gcgccaatcg cggatgaggg catcggcagt cacgatgcag ctcgagcgta
     8581 cgctctgatt gccgacccgg atctcctcgc tcgaatatga gcggatcaag ttgctcgctg
     8641 caccttccag cgtgaacttc atcgcgagct gctctcatgc gcaaacctca tgctgtgtac
     8701 gatacgccag cccgcgtgga attccgcgcc gccgggcgaa gtcacaccgt gcacgagatc
     8761 gcgctcgtct tccccgatct gtactttccg cgcgagttgg aacgcgacga ctcgcccgca
     8821 ctggctctgc ctgggctcga acacatcacg cgcttcgggc agcgctccgg cctcccgcaa
     8881 ggctggcgtc cctggctggc gaactgggca ggcagccccg agcttgcgag cctcccgccg
     8941 gcgctcgtcg ccgcgcggat tgctgcgtgc tctacgaccc cgacctcggt cgccggtgcg
     9001 agccttcctg gcggcgcagt ctggatggca acgcccgtgc acctggtcgc cagtctcacg
     9061 agcctgcatc tcgatcggcg cagtgtgctg tacctggcgg actcggaaga gctcgcgctg
     9121 tgtgcggatt ttgcccggac gtttcagggc tccggctacc acctcacgcc gctcgagggc
     9181 ggcggattgt tgctgttcgg ggccgcaaca tctgccgtcg aaacttgcga gccggcgcgt
     9241 gcgatcggcg agaacgtggc ggagcgcttg ccgagcgggc caggcgccgc ggcgttgagg
     9301 aaactcggcg cggagatcga gatgtggctt catggtcatc cactgaatga ggcgcgtctg
     9361 cgtcgcggcg agctcggcat cacgtcgttg tggttgtggg gtggcgggga gagcggtgag
     9421 cttgctcgcc ttcgcgagtg gacgcccgct gcgtccgccc gtgcccgagt tgcctttgga
     9481 tcagacgcat ctttgcgagc gctgtgggcg catatcgacg caaaagttca gcctttgccg
     9541 ccacagcttg cgcagatctt gagctatcct gcagaaggaa gcgctgtgtt ggtcatcgaa
     9601 gcggggcgga tgctgcactc gaatcagaat tggatgctgc ccgaggcgct ggcggagatc
     9661 gatcgccagt tcctggcgcc tgcggtgcag gcgctgcagc atcgccaggt cggcgtgctc
     9721 gaggtgctgg cgaacgactg gcgcgtgagc gtgcgcgcca cggatcgctt gcggctgtgg
     9781 cggcgagctc gacctggcct ttcggggttg cggtgaagtc tgacggtttg cgagtcgtgc
     9841 gccgcagctt cggcggcgat tgcgcagagc tcggcccgca actgcatccg gtcctgcagc
     9901 gcatttacgc cggacggggg gtccgttcgc caacggagct ggacacctca ctggaaagac
     9961 tgctgcctgt ggggagcctg gaggggatcg ctgcggcggt ggagctgctg ctgcggcacc
    10021 gctcgggagg acggatcctg gtcgtcggtg atttcgatgc cgatggtgcg accagcacgg
    10081 ccctggtgat tcgcgcgctg cgctgctggg gcttcgagtc ggtggacttt ctggttccta
    10141 accggttcga gttcggctat ggcctgaccc cggaaatcgt cgcgctcgcg gcccagcgct
    10201 caccgacgct gatcgtcacg gtggacaacg gcatcacgag cctggaaggg gttgccgccg
    10261 cacgcacgct cggcatcagt gtgctgatca ccgatcatca tctgccgggt gcccagcttc
    10321 cgcaggccga tgccatcgtc aatccgaatc tgcccggcag tcatttcggc agccgcgcgc
    10381 tggccggggt cggcgttgcg ttctatgtca tggccgccct gcagcgccgt ctggatgagc
    10441 tgcagctcac tccagcatcg gccgcccggg ctgcagagtt cctcgatctg gtggccctcg
    10501 gcaccattgc cgatctcgtg ccactcgatc cgaacaatcg cgtgctggtg ctgcaggggc
    10561 tgaagcgcat tcgcgcaggc aggtgcgtgc ccggcatcac tgcgcttctg gagatcgccg
    10621 cgcggcggcg cgccgacctc gatgcatccg atctggcgtt cgcggtggcg ccgcgtctga
    10681 atgcggccgg acggatcgat gacatgagca tcgggatcca gtgtttgctg gccgggcagc
    10741 ccgatgcggc gcgccagctt gccgagcgtc tggatgcgct caacgtcgag cgcaggacga
    10801 tcgaagcacg catgcagatg gatgcgctcg ccgccgtgcg ccggctcacg gatccgggtc
    10861 cgaagtcgat ccatcgctgt ggggtctgtc tctacgacga ttcctggcac cagggcgtcg
    10921 tgggcctggt cgccggccgc atggcggatc ggctgaggcg cccggtcgtg gcctttgcac
    10981 tcgcagcgga tgcgcagctg cgcggctcag cccgctccgt gcgcggcatc cacgtgcgtg
    11041 atgtgctcga tgcgatcgca acgcgagagc ccgggctgat cggtcgcttt ggcggccacg
    11101 cgatggcagc cggtctgacg ctcgagcggg gcaatctcga tcgttttgcg cgctgcttcg
    11161 atgaggaggt tgaacgctgg gccggcaact gtccggcggc cgatgccgtc gagactgacg
    11221 gtgagctgtc ggtgtgggag attgcgctcg agaccgctca tgccctgcgt gccggcggtc
    11281 cttggggaca agcatttccc gagccttgct tcgacggtct gtttgccatc cgcaatgcgc
    11341 gcgtggtggc tgaccggcat ctgaagatgt gggtggaggt gccgcgcacc ggacgctcct
    11401 tcgatgcgat cgccttcaat cacatcgaag aaggcagcga gttcacgcct ccgcagggcg
    11461 atgtgcagct cgtctaccgt ttggacataa acgaatatca gggggagcgc cgtctgcagc
    11521 tcctggtgga ccacgttatg agtggaaatc agggcccctg acggttagga tcgctggacc
    11581 tcactgcaat cgcgccggca tgcctgagca gggctctgcg tgccgccgaa accgttgtgc
    11641 ctatggaagt caatcagctc aaacgtcagc tcaaggatct cggggagcgg atcggctctc
    11701 ttcgggggtt tctttgagta cgaccagaag aaggagcgtc tggaggaagt aggccgcgag
    11761 ctcgaggatc ctgcggtctg gtccgatcag gggcgcgccc aggaactcgg tcgggagcgg
    11821 gcgcgacttg cggctgcggt gcaggagatc gatcgagcgg acaagggcat cgccgaagcc
    11881 gcggagctgc tggagcttgc cgagagcgaa ggcgatgagg ctgccgcgcg cgatatcgag
    11941 acggatgccg gcggattgga agcggacgtg cgccgtctcg agctcaagcg catgtttcgc
    12001 cacgagatgg attctcacaa cgcctttctg gacatccagg caggtgcggg cgggaccgag
    12061 gcgcaggact gggcgcagat gcttctgcgg atgtacctgc gctggggcgc cgcgcacggc
    12121 ttcagcagcg aggtgatcga ttccaacccc ggtgaggtgg ccggcatcaa gagcgcgacc
    12181 gtggagatca agggagacta tgcctacggc tggctgcgga ccgagacggg cgtgcaccgc
    12241 ctggtgcgca agtcgccctt cgattcgggc aaccgccggc atacctcgtt cgcctcggtg
    12301 ttcgtatcgc cggaagtcga tgaggacatc gacatcgaga tcaatcctgc ggatctgaag
    12361 accgatgtct accgctcctc gggagccggc ggccagcacg tcaacaagac ggagtcggcg
    12421 gtgcgcatca cgcacttgcc gagcggcatc gtggtggcct gccagaacga gcgcagccag
    12481 cacaagaatc gctccacggc gatgaagatg ctgaaggcca aactgtacga gctcgaggtg
    12541 aacaagcgca acgcggcggc ccgggtcctg gaggattcca agtccgacgt gagctggggc
    12601 aatcagatcc gctcctatgt gctcgaccag tcgcgcatca aggatctgcg tacgggcgtc
    12661 gaggtcggca acaccacggc ggtgctcgat ggcgatctgg atcagttcat ggaagcttcc
    12721 ctgaaagcag gcgtgtaggg tctgcaggta ctaccggcat gagcgagatg gacaaggcgc
    12781 aggatgagaa caagctgatc gcggagcggc gcacgaagct tgcagcgctg cgcgagggcg
    12841 ctgcagccgg cggcgcatat cccaacgact tccggcgcga ctcgctggcc gggcaactgc
    12901 acgatgcgtt cggtgagcgc gacgccgcgt ggctcgaggc caacccgact cgcgtgcagg
    12961 tgggcgggcg catgctgctc aagcgcccgc atggcaaggt gagcttcgcg cagatcgccg
    13021 atcgcaccgg ccagatccag ctcttcctgc agcacgatgc cctggggccc gcttacgagg
    13081 ccttcaaggg ctgggatgtc ggcgacatcg tcggagcgag cggtgtgttg ttccgcacca
    13141 agaccggcga gctctcggtg cgggccgaca gcctgcggct catcgtgaag tccctgcggc
    13201 cgctgccggg caagtggcat ggccttgccg ataccgagac gcgttaccgc cggcggtacg
    13261 tggatctgat cgtcaacgag acgagtcgca acgtctttcg ggttcgcacg cggatcatcc
    13321 gtttcctgcg cgactatctc gatgcactcg atttcatgga agtcgagacc ccgatgatgc
    13381 agccgatccc gggcggcgcg gccgcgcggc ccttcgtcac gcatcacaac gcgctcgatc
    13441 tggagatgta tctgcggatc gcccccgagc tgtatctgaa gcggctgatc gtgggcgggc
    13501 tcgagcgggt gtatgagatc aatcgcaact tccgcaacga aggtctctcg acgcagcaca
    13561 atcccgagtt cacgatgctc gagctgtatc tcgcctatgc cgactacact cagctgatgg
    13621 actgggtcga gaaggcgatc cgcgggatgg cagatgccgt gcacggctcg cgacagatgc
    13681 agtaccaggg gcggcagtac gatctggccg cccccttccg acgcgtgacg gtggaacagg
    13741 ccatcgtcgg ctgcaacccc ggcatcgatc cgctctcgct gcgcgatctc acctatctgc
    13801 gcaagctttg cgagaagctt ggtgtgccca ccggtgcgca ggacggtgcc ggaaagctgc
    13861 agatggagat cttcgacaag acggtcgagc acacgctgct cgatccgacg ttcgtgcacg
    13921 cgtacccggc agaagtgtcg ccgctgtcac gcgccaatga cggcgatccg ttcgtgacgg
    13981 accgattcga attcttcctc gccgggcgtg agatcgccaa cgggttctcc gagctgaatg
    14041 acccggagga gcaggcggcg cgtttccggg cacaggtggc ccgcaaggag cggggagacg
    14101 aggaggcgat gttctacgac gccgactacg tgcgtgccct ggaatacgga atgcctccgg
    14161 cggcgggtct gggcgtaggg atcgatcgcc tggtgatgtt cttcaccgac tctccctcga
    14221 tccgcgacgt catcctgttc ccgcacctgc ggcccgaggc cgactgacag agacgcggtc
    14281 agtcgggcaa cgcatagggc aggggcaaag cctcgacgct cagtgccgcc gcttccgagc
    14341 tctgaggcgc atccgcatcg ggtgtagctg aggcaccctc cggcaacgca gccacggcca
    14401 ggaattcaca gtagccctcc ggccgcgacg cagcgagcac caccttgaag gagcggccgt
    14461 cggcgagctg tccggcatcc cccggtgcga gccgacatgg cgtgcgagac aggaagcgct
    14521 gcatgcgtcg cttcacgcga ccgcgataat gcgcgcgggc aatcacttcc tgcccggtgt
    14581 agcaaccctt gtcgaaggcg atggccccga tcgcatccag gttgagcatc tgggcgacaa
    14641 actgctcgga ggtcgcggca gagatttgtg gaacgccctc ggcgatgtcc aggcgcagcc
    14701 aatcctgact tccatcgcgc tccgcccatc gatcgaaacc caactccggc gcagcagccc
    14761 ctgcgggaca taccagcagc cagcgagggc tcccccaacc aacctgggcc agcacaccct
    14821 cgcctagtcg agtctgcgca tcccgtgccg accgcaagct cgggcccgct ggtgccgcaa
    14881 gctcgccgat gcagccaccc acgcgccagc tctcgagctc ggcggtgatc tgtaccttgg
    14941 cacgcaggat gtatcgcgcg aggcggctca cgagggtcgc agcaagatcg cgcgggacga
    15001 tggcgagaat gtcctgagca tcgagctgca gcagccgcag cagcgcaatg gcccggccct
    15061 ggggattgtg gtagccgccc agcagtgagc gctcggccga aagctgcgac acgtcgctgg
    15121 aaagctggcc ctgcaggaag cggatgacat cggggccgcg catgcgcacg attcccagct
    15181 catccagagc gatcagttcc cgactttgca tgctgccgtt ttacgttggt tttccagtgg
    15241 tttgcaatgg tcgcgcgagt cgaattctgg cagactggcg gggtatgcag cagcttgccg
    15301 atgctcagcc cggagaaacg ctccggcatc ttccgttccg ctcgcttccg cctcccgaaa
    15361 cgccaagcca agtggccccg ccagggttgc ggccgcgcag cgctcgtagc tgatgcgcgc
    15421 acgtcccttg tcaccccacc tgtcggtgta ccggatgagc cggtacaccc tggtcacttc
    15481 cattctcaat cgattcacgg gcgcggggct ctccgtcggt ctgctcctct tcgtctattg
    15541 gctgatggcg gtggccagcg gtgggcgcgc ttacgcgcaa gcccagcatg tacttgcgca
    15601 tggaattttc aaggtgctgt atgcagcgct cgtgatcgcg gtgtgctatc acctggtcgc
    15661 cggcatccgg catctggtct gggacacggg ccacgggctc gagcgcgcac agtcacagcg
    15721 aagcgcgtgg ctggtcatcg ccgtgagcct cgttctgatc gcgctcgtcg ggttctgcct
    15781 gctgtccgcc cggggacagg gcgcatgagc agcctgcgca gtcctctggg ccgagtggcc
    15841 ggactgggtg ctgcgcgcgg tggcgtgcag cactggtggc tgcagcgcct gacttcgatt
    15901 gcgctcgtgc cgctcgccgt ctggttcctg gtctgcctgc tgaggctacc ggcgtttgat
    15961 cacgcaaccg tgacaggatg gatgtcgcag acctggacag cggttctact gatccttctg
    16021 ctgctcgtcg cctgctggca ttcgcaaatc ggcatacggg tgatcgtcga ggactacgtg
    16081 catggcgcgg gcgcccggac actgacactc gtgatcgtca ctttcgctca taccttcata
    16141 gcgttcgccg gagtgtttgc gatcctcaag gtggccttcg gggcggcccg atgagcacct
    16201 acgagttcat cgaccacact tacgacgtca tcatcgtggg cgcaggcggt gccggtttgc
    16261 gcgcgacgct gggcctggcc gaagcggggc tgtccaccgc ctgcatctcc aagttgtttc
    16321 ccacgcgcag tcacacggtc gccgctcagg gcggcatcag cgccgcgctg ggcaacatgg
    16381 gcgaggatga ctggcgctgg cacatgtacg acaccgtgaa gggctccgac tggctcggcg
    16441 atcaggatgc gatcgagttc atgtgcaagg aggc
//
LOCUS       DASRHN010000431        18450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_652732, whole genome shotgun sequence.
ACCESSION   DASRHN010000431 DASRHN010000000
VERSION     DASRHN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652732"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFB37_17510"
     CDS             <1..654
                     /locus_tag="VFB37_17510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO24294.1"
                     /translation="TARGWAKGAVLILATGAYTDTLADSLRRTLILVRSAQVATVPLP
                     AQLRASILPEGQAASDTCHLLRYFRLDASGRLIMGSRGSFDAVPVEPAARHHYRAVAE
                     IYPQLEGIPYEYHWSGRVGLTRDHLPHLHEMAPGLLAALGYNGRGVAMATLMGQLLAR
                     RAQGEPAEELGFPFSAVKPIPLHRFSQLGTRMVVQYLRALDGIARLRVRLAMAAKTA"
     gene            671..1594
                     /locus_tag="VFB37_17515"
     CDS             671..1594
                     /locus_tag="VFB37_17515"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZO24295.1"
                     /translation="MSWPKIGLLAALVIVNIAFVIEWLRARRTHPMRDRPTVGDVLIG
                     FFTDFFDGLGIGSFAPTTALFKFRRRPADELIPGTLNVGHNASAFIETVVFVTAVAVE
                     PMLLTAMIASATIGAWLGAGVVSRLPRRAIQVFMGIALLSAATFFLINNLGALPAGGT
                     AMSLPGWRFVVAVAVNFVLGALMSVGIGAYAPCMVLLALLGLHPLGAFPIMMGTCGLV
                     QPVASLRFFKTGRFAWGPSLGLTIGGIVGVLIAVFIVKSLPLSALRWLVMFVVAYAAI
                     SMLYSAYSSRPAAGATTAAQTGAAPAQPGSL"
     gene            1602..2153
                     /locus_tag="VFB37_17520"
     CDS             1602..2153
                     /locus_tag="VFB37_17520"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HZO24296.1"
                     /translation="MISLAVDPAVAALAVTCLALLFAAAALHKLRDLRVFAEIFAAYE
                     ILPGLVRLHLTALVPALEAGVAAGLLLKSTRPAAAVLAVTLLLGYAASIGINLRRGRR
                     DLACGCGGPNERRPIAPWMIARNLLLAALAGLVLFPLTARSLELTDAVTISFGTLTCA
                     LIYLCLDTLLGRSGLQSAELRQT"
     gene            2153..2746
                     /gene="mauD"
                     /locus_tag="VFB37_17525"
     CDS             2153..2746
                     /gene="mauD"
                     /locus_tag="VFB37_17525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase accessory protein
                     MauD"
                     /protein_id="HZO24297.1"
                     /translation="MTALAISNIVLWVIVLALLVVVLALTRQVGVLHERISPAGALML
                     NRGLSVGEQAPALDVVDLEGRGLRIGAPRSDGLSTLLLFVSPSCPVCASLLPVLKSSR
                     KHEQSWLQIVLASDGEPAAHREFVRSHGLTGLPYVVSAPLGITYQVNRLPYAALLDES
                     GTLRARGLVNSREHLESLFEAKRLGVASLQEYFASRA"
     gene            3023..4456
                     /locus_tag="VFB37_17530"
     CDS             3023..4456
                     /locus_tag="VFB37_17530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZO24298.1"
                     /translation="MNGCAVGPNFHSPAPPATTHYTPGAQPEATVEAPVPAGAAQKFI
                     ADRDIPGDWWSLFQSEPLDGLVRQALHDSPTVEAAKAALRAAQDTYVAERGALLLPTA
                     DGQLAATRERVPGAAFGQPSLPASTFTLYTASVNVSYRLDLFGASRRQLEGLRAQADY
                     QRWELEAADLTLTGNVVTTAFDVASLRAQLAAVNDVVVSEGAQLKVIQRQFDVGGVSR
                     SDILSQQAQLAQAQAQVPALEKALAQAQHRLAVLAGRTPDDATLPDFTLDQLALPTEL
                     PVTVPAKLIRQRPDVQAAEALLHQASAQVGVATANLFPQLNLTGTIGSESLSSQGLLG
                     PGTGAWSAGAALLQPLFHGGELRYKRRAAIDSLDQAAANYRQAVLSAMQDVADTLRAL
                     EADARDLRAQVEAERTAADALAIAQKQFRLGGVSYLTVLNAQRLYSQSRLSRVQSQAT
                     RYADSAALFQALGGGWWNRKDTEGAGK"
     gene            4462..5619
                     /locus_tag="VFB37_17535"
     CDS             4462..5619
                     /locus_tag="VFB37_17535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO24299.1"
                     /translation="MRRRLIIVVALAGLLLVLLVGFNMVKQHFMSQARLQNASPPQTV
                     SATSARYSDWQPHVEAIGTLRATLGVDVTTEVTGLVRSLHFKSGDLAKEGEILVELNA
                     DADIAQLHALEAAADLSSTVYARDKLQYQDQAISKAQLDADAADLKNKRAMAAAQAAL
                     VAKKTLRAPFAGQLGITTVNPGQYLNTGDKVVTLQSVDPIYVDFKLPQQELARISTGQ
                     SVALTTDSYPGVIFAGKINALDPKIDASTRNFQAEATIANPEHRLLPGMFARVSVSYG
                     GTTRYLTLPQTAITYNPYGTTIFLAQHRAPEANQKPPKKGQPPPPTLVAQQVFVTLGP
                     TRGDQVAVLKGIKEGDMVVTSGQLKLNNGTPLLLDNRVAPADDPNPAPQEQ"
     gene            5629..8718
                     /locus_tag="VFB37_17540"
     CDS             5629..8718
                     /locus_tag="VFB37_17540"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO24300.1"
                     /translation="MSSFTDIFVKRPVLSLVVSLMILVLGVRAIFELSVSQYPETENA
                     VVTISTTYYGADAATVAGFITQPLEAAISQAQGIDYLSSNSSTGVSVIIATLRLNYDS
                     NRALTEINTKVASVRNQLPPQAQQPVLTVQVGQTIDAMYIGYYSDTLAPNGLTDYLYR
                     VVKPQLDALEGVQTAEIIGGRQYALRAWLDPARMAAHGVAANDVYAALSANNYLTAVG
                     NTKGQMVSVDLSASTDLHSVDEFKHLIVRQQNDAIVRLEDVATVVLGAETYDFSVAFN
                     GRRSVFLGIQVSPKANILDVATLVKKTLPGIASQLPSGVSQKLVYDATEFIHTSIVEV
                     IKTLVEALLIVTAVIYLFLGSVRAVIVPLVAMPLSLIGAFFLMLALGYSINLLTLLAL
                     VLAIGLVVDDAIIVVENVDRHMKEGQTPFEAALQGARELGGPIIAMTVVLVAAYLPIG
                     FQGGLTGALFTQFAFTLAGAVTVSAIIALTLSPMMCANFFRMEQEEGRLARFIDRRFT
                     SLRTRYQRLLSKGLDTWQVFVTLGVLLLAGTVYLFMTSATELAPQEDQGVVLYQAIGP
                     PDATLNQMQAYSQQLSTAARSLPEFLSSFQITGTPSVNQGFGGFLFKPWDQRSRSADE
                     LQKVLQDKWNHIAGAHIAAFQFPSLPGSQGLPLQFVITTTQPIQNLDEVANKVLEKAK
                     ASGMFWFVDSDLKLDKPQATLKVDRDLVADLGLTQQDVGNALGAALGGGYVNYFSLAG
                     RSYKVIPQVLQKDRLNPNQVLDYYIRAGDGTLIPASTVAHIETSTVPEAINHFQQLNS
                     ATIAGAPGVSQADALKFLRQALHEVAPSGYNVDYSGQSRQYVRGSSGFVVTMLFALVI
                     VYLALAAQFESFRDPAVILVSVPMALFGALLFINIGFATLNIYTQVGLVTLLGLISKH
                     GILIVQFANELQREGRTKLQAIIEAAGVRLRPILMTTAAMVLGVVPLVIASGAGAAGR
                     RAMGIVLFTGLSIGTLFTLFIVPAVYILIGADHAKDRLKRSKKDSREGHSAPPEALA"
     gene            complement(8803..9168)
                     /locus_tag="VFB37_17545"
     CDS             complement(8803..9168)
                     /locus_tag="VFB37_17545"
                     /inference="COORDINATES: protein motif:HMM:NF009508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HZO24301.1"
                     /translation="MKHFLLFYEVSENYLARRPEFRHAHLAKAWAAHRRGELLLGGAL
                     NDPLDGAVLLFAGEAREVAEEFARTDPYVINGLVSRWRVREWATVAGAGAANPVYADG
                     PDLGASRPNQASPPNRNPR"
     gene            complement(9174..10778)
                     /locus_tag="VFB37_17550"
     CDS             complement(9174..10778)
                     /locus_tag="VFB37_17550"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZO24302.1"
                     /translation="MTLETARQLLHNNVPPSQDLHDLDAMMRNPTELSSELPTVSHWI
                     GGRRAASNPARTGNVFNPARGAVAARVAFASKEEVNAAVAAAREAARGWASTPAPRRA
                     RVMFRFKELVEHAAGEIAHLISREHGKTVSDAHGELARGLEIVEFACGIPHLLKGEFS
                     ENVGTGVDTFSLRQPLGVVAGITPFNFPAMVPMWMFPIAIACGNTFVLKPSERDPSTS
                     VRLAELLKEAGLPDGVFNVIQGDKEAVDALLYHPDVAAISFVGSTPIARHIYLEGTSH
                     GKRVQALGGAKNHLVVMPDAELDLVTDALLGAAFGSAGERCMAISVAVAVGDETADRL
                     VAKLVPRIKGLRIGAPDDEQTEMGPLVTREHLDRVRGYIEAGVAEGASLVLDGRGTAL
                     PGAQEGFFLAGSLFDRVRPDMKIYREEIFGPVLVIVRVPHADAAIRLINSHTYANGTS
                     VFTRSGDAARAFATAIEVGMVGVNVPIPVPVAFYSFGGWRNSLFGDHHIYGEEGVRFY
                     TRLKCVTQRWPRTPGSDAEFTMPVMR"
     gene            10866..12581
                     /locus_tag="VFB37_17555"
     CDS             10866..12581
                     /locus_tag="VFB37_17555"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZO24303.1"
                     /translation="MNKRSVSRRAFVGFAGAGIATLIRSPAALAALQGEPRDPDLIVF
                     NAKVYTVDAKRPKAEAFAVKAGRFIAVGSTEDIKSLAGKSTQSFDAQQMTIVPGFIDC
                     HNHATGNMLLYDVLVGNPYEVEFVTISSIVEKLRARAQKTPAGFWVEGYFYDDTKVKD
                     GRQINIHDLDQVSRQHPVGVEHRGGHTHFFNSKAFELAGVTRATPDPPGGTFDRGPDG
                     ELNGRVTDNALAAFEKVGKRPAFSEAERRQRDRDGLAHISKQFVRYGLTSVHHEEGDF
                     AALQEIRARGQLLHRVSYEMDDGMLEAMLANGIETGFGDEWLRLGATSEHQVDGSFSE
                     RTMALSVPYPGSKSGYRGNLTETQATLDGWIERVWRAGIQPNCHANGDVAIDAVLRSL
                     QRAQERFPRADVRPKITHCTLINDDLLKRMKVLGVVPTPFTSYAYYNSDKFHFYGEAL
                     MRHCMAYRSFIDSGITAAAGSDFPPGPFAPLMGIQGMVTRTGWNGETWGANQRVTVAE
                     ALQINTLNGAYASHEEKSKGSITPGKLADFVVLAEDLNTVPQDHIKDVKVFRTVTEGA
                     TVYQA"
     gene            complement(12550..13614)
                     /locus_tag="VFB37_17560"
     CDS             complement(12550..13614)
                     /locus_tag="VFB37_17560"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZO24304.1"
                     /translation="MAILKSSCDLLLKAGVLAINALGFAQASAAAHPYPDHPVHLVVP
                     YPVGGGADHWGRLLAAGLAERLGQPIIVDNVPRKGGNEGTALVAHAPPDGYTLLLGSI
                     GPLVVHRYTYTSLPFDPERDFVPIALLESSPMLLVAAASVPVVSARELIDLARARPGS
                     LSYASNGIGSPEEVAGELFKARLNIDLHHIPYDGAGPARKAVLAGQAGLMFDPCKGAM
                     PGIRSGLQNPLAVAAANRLASLPRVPTFAEVGVTDYELRIWTGLLAPAGTPPAIVSRL
                     NRMIQEVLRSDDIKTEIAEEGGEVGATTPESFAAFIESERRRWSALVRESGVPRVDVR
                     AGALVDFRSELRPDTPWHPQ"
     gene            complement(13700..14959)
                     /locus_tag="VFB37_17565"
     CDS             complement(13700..14959)
                     /locus_tag="VFB37_17565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HZO24305.1"
                     /translation="MRTSIGLLGALCGALVSTVALSQTDPVLEWNAIMVSTTASQNPF
                     FQARSAAITQLAVFEAVNTIEKHFDPYLGTLTAPSGASPEAAAIAAAHDVLLYYFPIN
                     QGALDSAEGVSLARIPDGSAKQDGIGVGEAAAAAFIQLRSSDGSLPMQFYVPGSSDPG
                     QWQTTPSCPPAGGTLLQWPHLQPFAIVSTDQFRSAPQPALTSMRYAIDFDEVQRVGSA
                     DSSFRPQDRADVARFYAATPPVTVWNSAAAQVAVERGRSLSENARTLALMDMAISDAA
                     GSVFDTKYLDHFWRPETAIHASGNTSWVPYIKAPCFPAYGSAHASLSGAARKVLERIY
                     GRDHHMITLTNSTVPGVTLQYETFEQITHDINDARVFGGIHFRFDQVAGGEQGRRVGA
                     YVYEHTLRRAHGDGDHDGDDDGDRSKE"
     gene            15109..15660
                     /locus_tag="VFB37_17570"
     CDS             15109..15660
                     /locus_tag="VFB37_17570"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZO24306.1"
                     /translation="MGQLTDLLGRVQGGDAEARAALFAAAYTALHDLAQARLRRGGRH
                     TLLDTTGLVHESYLRFARAGELRAEDRRAFFAYASQVMRSIILNSVRERVALKRGAGQ
                     SAQASNPEESPDLVSEERVVLEVHEALKDLEKADPRLAQVAEMRYFGGYSEKEIAETL
                     EVTERTVQRDWEKARLILAAALR"
     gene            15738..>18450
                     /locus_tag="VFB37_17575"
     CDS             15738..>18450
                     /locus_tag="VFB37_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HZO24307.1"
                     /translation="MSRLLDEALPLDADGRRVWLAALPAEQRDLAQSLRDALLPGDVE
                     RAQIEKLASLPSLGAASQAEAPPTSGLHTGDRVGPYELIRPLGTGGMAEVWLARRADG
                     AFRREVALKLPMSSCPRPDLEQRFARERDILASLEHPHIARFYDAGVGSGGLPYLSME
                     YVEGRPLTEWCDAHRLGIRPRLELFLQMLEAVQYAHEKQVIHRDLKPSNVLVTESGQI
                     RLLDFGVARLLQADEPRTELTHVHGRALTPDYASPELLRGESVDARGDVYSLGVLLYE
                     LLTGVRPYRLTQAASIGLLEHAIVTVQVKKPSTQLEANAVVSRDTTPEKWARQLRGDL
                     DAIALKALAKEPAGRYPSAAALMEDLRRYLNARPVQALPARLTDRLRKFLRRNRVTVG
                     VTVTAVAIVVAAVTLAVVRQPGQGPSRLWLDPLARARVLRLTDFAGTEQAAAISRDGR
                     FAAFLAARNGPLDLWLTEIGTNHYRNLTEGRISQLRNPEIRSVGFSPDGSLVTFWARN
                     GDGSRAQDINVMAVPTAGGAVQPYLPEAAELDWSADARQVVFHTTAPGDPLFVRAATE
                     AKAHRIYVDAPGVHCHFLTWSPDGEFIYFVRGDPPSADWDIWRLRPSGAGLERLTSQH
                     AWVTYPVLLDARTLLYLATDADGSGPWLYVLDLVSKRSRRVSVGLERYTSLSANADRT
                     RILATVADSRSELWRAAIGNSGSPQATAERIAGVSQSASAPRIGPGYIAYVSSLGARR
                     GIWKYANRTATELWSDATADRVGALSISPDGRRIAFVVDRGGTTQLYAVDSDGRNPRL
                     VAQTLTLRGDLAWAPDSQSILSAIESDGEPRLARIFLDSSPPQPMVSEYSVDPAWSPD
                     GKYFVYSGAQVATIFPLRASAPDGRPYGMPGMILTIGARRVAFARNSG"
BASE COUNT         3178 a         5969 c         5909 g         3394 t
ORIGIN      
        1 acggcgcgag ggtgggcgaa gggggctgtg ctgattctgg ccacgggtgc ctacacggac
       61 acgctcgctg actcgttgcg tcggacgctg attctcgttc gttccgccca ggtggccacc
      121 gtgccgctgc ctgcacagtt gcgcgcctcg atcctgcccg agggacaggc agcttccgac
      181 acctgtcatc tgctgcgtta ctttcgcctg gacgccagcg gccgtctgat catgggctcg
      241 cgcgggtcgt tcgatgccgt ccctgtcgaa cctgccgcac gacatcacta ccgggcggtc
      301 gcggagatct atccgcagct cgaagggatc ccctacgagt atcattggag cggcagggtc
      361 ggcctgacgc gcgatcacct tccgcacctg cacgagatgg ccccaggcct cctggcggct
      421 ctcggctaca acggccgcgg cgtggcgatg gccacgttga tgggccagct gctcgcccgc
      481 cgggcacagg gggaacctgc cgaggagctc ggctttccct tcagcgccgt gaagcccatt
      541 ccgctgcatc ggttcagcca gctgggcacg cggatggtcg tgcagtactt gcgcgcgctc
      601 gatggcatcg cgcgcctgcg agtccgcctc gcgatggcag caaagaccgc atgatggccg
      661 gggaggcgac atgagctggc ccaagatcgg acttctcgcc gccctcgtga tcgtcaacat
      721 cgcgtttgtc atcgagtggc tccgtgcccg gcgcactcac ccgatgcgcg accgaccgac
      781 cgtcggtgac gtgctgatcg gtttcttcac ggatttcttc gacggcctcg gcatcggctc
      841 cttcgcgccc accacggcgc tgttcaaatt ccgccgccgc ccggccgatg agttgattcc
      901 cggcacgctc aatgtcggcc acaacgcctc ggcattcatc gagaccgtgg ttttcgtgac
      961 ggccgtggcc gtggagccga tgctgctcac ggcaatgatc gccagcgcga ccatcggcgc
     1021 gtggctcggc gccggggtcg tgagccggct gccgcggcgg gccatccaag tcttcatggg
     1081 cattgcgctg ctgagcgccg ccactttctt tctgatcaac aatctcggtg cgctgccggc
     1141 cggcgggacg gccatgtcgc tgccgggctg gcgattcgtg gtcgccgtgg ccgtcaactt
     1201 cgtgctcgga gccctgatga gcgtcggcat cggcgcgtac gccccatgca tggtcctgct
     1261 ggcgctgctg ggtctgcatc ccctgggtgc attccccatc atgatgggga cctgcgggct
     1321 ggttcagccg gtggcgagtc tgcgcttttt caagacgggt cgtttcgcgt gggggccttc
     1381 actcggtctc acgataggtg ggatcgtggg cgtactgatt gccgtcttca tcgtcaagtc
     1441 gctgccactg agtgccttgc gttggctggt catgttcgtg gtcgcctacg ctgcgatctc
     1501 gatgctctac tcggcctaca gcagcagacc ggcggcgggt gcgacaacgg ccgcgcaaac
     1561 cggcgccgcg ccggcccagc ccggtagtct atgatgcggt gatgatcagc cttgccgtcg
     1621 atccggccgt ggccgccctg gcagtcacgt gcctcgctct gctgtttgcc gccgcagcct
     1681 tgcacaaact gcgcgacctg cgcgtgttcg cggaaatctt tgccgcttac gagatcctgc
     1741 cgggcctcgt gcgattgcac ctgaccgcgc tcgtgccggc actcgaggcg ggcgtagcgg
     1801 ccggcctctt gctgaagagc acgcgccccg cggcggccgt cctcgctgtg accctcttgc
     1861 tcggctatgc ggcctcgatc ggcatcaacc tgcggcgggg acggcgcgat ctcgcctgcg
     1921 gctgcggcgg gcccaacgag cgtcgcccga tcgccccctg gatgatcgct cgcaacctgc
     1981 tgctcgccgc gctcgcgggg ctcgtcctgt tcccgctcac tgcacgctcg ctcgagctga
     2041 ccgatgccgt gaccatcagc ttcggaacgc tcacgtgtgc actcatctat ctgtgcctcg
     2101 acacgctgct cgggcgcagc ggcctgcaga gcgcagagtt gcggcagacc tgatgacagc
     2161 gctcgccatc tccaacatcg tactgtgggt gatcgtcctc gcgctgttgg tggtcgtgct
     2221 cgctctgacc cgccaggtgg gtgtgttaca tgagcgcatc tcaccggcgg gcgcactcat
     2281 gctcaaccgt ggcctttcgg tgggcgagca ggcacccgcg ctcgatgtgg tggacctgga
     2341 gggaaggggg ctgcgcatcg gcgctccacg aagcgatggc ttgagcacct tgctcctgtt
     2401 tgtctcgccg agctgtccgg tctgtgcctc gctgctgccg gtgctcaagt ccagccgcaa
     2461 gcacgagcag tcctggctcc agatcgtgct ggcgagcgat ggcgagcctg cggcgcatcg
     2521 cgagttcgtg cgcagccatg gattgacagg cttaccctac gtcgtgtcgg ccccgctcgg
     2581 catcacctac caggtgaaca ggcttcccta cgctgcgcta ctggatgagt cgggcacgct
     2641 tcgcgcgcgc ggacttgtca actcccggga gcatctggag agcctgttcg aggccaagcg
     2701 cctcggagtt gcctcgttgc aggagtattt cgcaagtcgc gcatgagtct ctctctttcg
     2761 agagaggctc gcccgtggcg ggattggagg atcggttccg ccagtatcag agcgccgcag
     2821 cgcacgttcg ggccgccgca gcttgattgc ggctgatcct gccagacgtt agagtaccgg
     2881 ccccttggcg gagttgatcg gcaccgccga tcccgtgccg cccctgccaa cacagatccc
     2941 ggacagctga gcagggcctt tgcaactctc aacgcacacg ccgcgaaaat cgattgttgg
     3001 cgccatcgcc gccctactgg gcgtgaacgg atgcgcggtg gggccgaatt tccactcgcc
     3061 ggcgcctccg gcaacgaccc actacacccc gggagcgcaa cccgaggcca ccgtggaggc
     3121 gcccgtgccg gcaggcgctg cgcagaagtt cattgcggat cgcgacatcc cgggcgactg
     3181 gtggagtttg tttcagtccg agccgctcga tggcctggtc cggcaggcct tgcacgacag
     3241 cccgactgtt gaggcggcca aggctgcgct gcgcgccgca caggacacct acgtcgctga
     3301 gcgcggcgca ctgctgctgc ccaccgccga cggccagctc gctgcgacgc gtgagcgcgt
     3361 gcccggcgcg gctttcggtc agccgtcgct gccggcaagc acgttcacgc tctacaccgc
     3421 ctccgtcaat gtctcgtacc ggctggacct cttcggcgct tcccggcggc agctcgaggg
     3481 actgcgtgcc caggcggact atcagcgatg ggagctcgag gcggccgacc tgacattgac
     3541 aggaaacgtg gtgacgacgg ccttcgatgt cgcatccctg cgcgcgcagc tcgcggccgt
     3601 caatgatgtc gtggtgagcg agggcgctca gctgaaggtg atccagcggc agttcgatgt
     3661 cggcggagtc tcgcgctcgg atattctctc gcagcaggcg cagctggcgc aggcccaggc
     3721 gcaggttcct gctctggaga aggcgcttgc gcaggcccaa catcgcctcg cggtgctcgc
     3781 cggacggacg cccgatgatg cgaccctgcc ggacttcaca ctcgatcaac tcgcgctgcc
     3841 aaccgagctg cccgtgacgg tgccggcgaa gttgatccgc cagcgtcccg acgtgcaggc
     3901 tgccgaagca ctgctgcatc aggcgagtgc acaggtcggt gtcgccaccg ccaatctgtt
     3961 tccgcagttg aatctgaccg gcacgatcgg atcggagtcg ctgagctccc agggattgct
     4021 ggggcccggc acgggcgcgt ggtccgctgg cgcagcgctg ctgcagccac tgtttcatgg
     4081 gggtgagctg cgctacaagc gtcgcgccgc cattgacagt ctcgatcagg ctgctgcgaa
     4141 ttaccgccag gccgtgctct cggcgatgca ggacgtcgca gacaccctgc gcgcgctgga
     4201 agcggacgcg cgtgatctga gggcgcaggt cgaggccgag cgaacggctg cggacgcgct
     4261 ggcgatcgcg cagaagcagt tccgcctcgg gggcgtcagt tatctcaccg tgctcaatgc
     4321 gcaacggctg tactcgcagt cgcgtctcag ccgcgtgcag tcgcaggcga cccggtacgc
     4381 ggattcagcg gccctgtttc aggcgctcgg cggtggttgg tggaatcgca aagacacaga
     4441 aggggccggg aagtgagcgt catgcgccgg aggttgatca tcgtggtggc ccttgcgggg
     4501 ctgctgctcg tgctgctggt gggcttcaac atggtcaagc agcacttcat gtctcaggcg
     4561 cgcctgcaga acgcctcgcc gccgcagacc gtgagtgcca cgagcgctcg atactcggac
     4621 tggcagcctc atgtggaggc gattggcacg ctgcgagcca cgctcggagt ggatgtgacc
     4681 accgaggtga cgggcctcgt gcgctcgctg cacttcaagt cgggagatct cgcgaaggag
     4741 ggcgagatcc tggtggagct gaacgccgac gcagatattg cgcagctgca cgcgctggaa
     4801 gcggccgccg atctctcctc gacggtttac gcgcgcgaca agctgcagta ccaggatcag
     4861 gccatcagca aggcgcagct cgatgccgat gcggccgatc tgaagaacaa gcgcgccatg
     4921 gcggcggcgc aggcggcgct cgtggccaag aagaccctgc gcgcaccctt tgccggtcaa
     4981 ctcggcatca ccaccgtcaa tcccgggcaa tacctcaaca cgggcgacaa ggtcgtgacg
     5041 ctgcagtcgg tggatccgat ctacgtcgat ttcaagctgc cgcagcagga gctggcccgc
     5101 atcagcaccg gacagagtgt ggctttgacg acggactctt atcccggcgt catcttcgcc
     5161 ggcaagatca acgccctcga cccgaagatc gatgcgagca cgcgtaattt ccaggcggag
     5221 gcgaccatcg ccaaccccga gcaccggctt ctgcccggga tgtttgcgcg cgtttccgtg
     5281 tcctacgggg gtaccacacg ctatttgacg cttccgcaga ctgcgatcac ctacaacccc
     5341 tacggcacca cgatcttcct tgcacagcac cgcgcgcctg aggccaacca gaagcccccc
     5401 aaaaaaggtc aaccgccacc gccgacgctg gtggcgcagc aggtgttcgt gaccctcggg
     5461 cccactcgcg gcgatcaggt ggccgtgctg aagggaatca aggagggcga catggtggtc
     5521 acgagcggcc agctcaagct caacaacggc acaccgctgc tgctggacaa cagggtcgcg
     5581 cccgccgacg accccaatcc tgcaccgcag gagcaatgag gcgctgcgat gagcagtttc
     5641 accgatatct tcgtcaagcg gccggtgctt tcgctggtcg tgagcctgat gatcctcgtt
     5701 ctcggggtcc gcgcgatatt cgagctgtcg gtcagtcagt atccggaaac cgagaatgcg
     5761 gtcgtcacca tctccaccac ctactatggc gccgatgcgg ccaccgttgc gggattcatc
     5821 acgcagccgc tcgaagctgc catctcccag gcgcagggca tcgactatct gtcttccaac
     5881 agcagtaccg gcgtgtcggt gatcatcgcc acgctgcgtc tgaactatga ttcgaaccgc
     5941 gcgttgacgg aaatcaacac caaggtcgcc tcggtccgca atcagctgcc tccgcaggcg
     6001 cagcagccgg tcctcaccgt gcaggtcggc cagaccatcg atgcgatgta tatcgggtac
     6061 tacagcgaca cgttggcgcc caatggcctg accgattatc tctaccgggt cgtaaaaccg
     6121 cagcttgacg ccctcgaagg cgtgcagacg gccgagatca tcggaggccg ccaatacgcg
     6181 ctgcgcgcct ggttggatcc ggcccgaatg gccgcccacg gcgtggcggc caacgatgtg
     6241 tatgcggcgc tgtcggcgaa caattatctg actgcggtgg gcaacaccaa ggggcagatg
     6301 gtgagcgtcg atctgtcggc gtccaccgat ctgcattcgg tcgatgaatt caagcacctg
     6361 atcgtgcgcc agcagaacga tgcgatcgtt cgcctggagg atgtggccac cgtcgtgctc
     6421 ggtgccgaga cctacgactt cagtgtcgcc ttcaacggcc ggcgctcggt attcctgggc
     6481 atccaggttt ccccgaaggc gaacatcctc gatgtcgcca ctttggtgaa gaagacgctt
     6541 cccgggattg cgagccagct cccgtccggg gtctcgcaga agctggtgta cgatgccacg
     6601 gaattcattc acacctcgat cgtggaggtc atcaagactc tggtcgaggc gctgctgatc
     6661 gtcacggcgg tcatttacct gttcctcggc agcgtgcgcg cggtgatcgt cccactcgtg
     6721 gcgatgcccc tgtcactcat cggcgcgttc tttctgatgc tggcgctggg ctattcgatc
     6781 aacctgctga cgctcctcgc cctggtgctc gccatcggcc tggtcgtgga tgatgcgatc
     6841 atcgtggttg agaacgtcga ccgacatatg aaggaggggc agacgccctt cgaggccgcg
     6901 ctgcagggcg cgcgcgagct cggcggcccg atcatcgcca tgaccgtcgt gctggttgcg
     6961 gcctatctgc cgatcggctt tcagggcggc ctcacgggag ccttgttcac gcaatttgcc
     7021 ttcacgctgg caggagccgt gaccgtctcg gcgatcatcg cgctcacgct ctctccgatg
     7081 atgtgcgcga acttcttcag gatggagcag gaagagggcc gcctggcgcg ctttatcgac
     7141 cgccggttca cgagcctgcg cacacggtat caacgtctgc tctcgaaagg actcgatacg
     7201 tggcaggtgt tcgtcaccct cggcgtgctg ctgctcgccg gcacggtata cctgttcatg
     7261 acttcggcaa ccgagctggc accgcaggag gatcaggggg tcgtgctcta tcaggcaatc
     7321 gggccgccgg atgccacgct caaccagatg caggcctatt cgcaacaact ttcgaccgcg
     7381 gccagaagcc tgccggaatt cctgtcctcg ttccagatca ccggaacgcc ctcggtgaac
     7441 cagggattcg gcgggttcct gttcaagccc tgggatcaac gctcgcgcag cgccgatgag
     7501 ctgcagaagg tcctgcagga caagtggaac catatcgccg gcgcccacat cgctgctttc
     7561 cagttcccct ctctgccggg ctcgcagggc ctgccgctgc agttcgtcat cacgacgacc
     7621 cagccgatcc agaacctgga cgaggtggct aacaaggtgc tcgagaaagc gaaggcgagc
     7681 ggcatgttct ggttcgtgga ctcggatttg aagctcgaca agcctcaggc gaccctgaag
     7741 gttgaccgcg acctcgtggc cgacctcggc ctcacccagc aggacgtcgg caatgcgctc
     7801 ggcgccgcgc tcggcggcgg ctacgtcaat tacttctcgc tcgccggccg ctcgtacaag
     7861 gtgatcccgc aggtgctgca gaaggatcgc ctgaacccga accaggtgct cgattactac
     7921 atccgcgcag gcgatggcac gctgataccc gccagcacgg tggcgcacat cgagaccagc
     7981 accgtgccgg aggccatcaa tcacttccag cagctcaact cggcgaccat cgccggcgca
     8041 cccggcgtct ctcaggcgga tgcactgaag tttctgcgcc aggcgctgca tgaggtcgcg
     8101 ccgagcggct acaacgtgga ctattccggc cagtcccgcc agtacgtccg cggctccagc
     8161 ggcttcgtgg tcacgatgct gttcgcgctc gtcatcgtgt atctggcgct ggcggcgcaa
     8221 ttcgaaagct tccgcgatcc ggcggtcatt ctggtgtcag taccgatggc cctgttcggc
     8281 gcgctcctgt tcatcaatat cggtttcgcg acgctgaaca tctacacgca ggtcggcctc
     8341 gtgaccctcc tcgggctgat cagcaagcat ggcatcctga tcgtgcagtt cgcgaacgag
     8401 ttgcagcgcg agggccggac gaagctgcag gcgatcatcg aagcggcggg ggtccggctg
     8461 cgcccgattc tcatgacgac cgccgcgatg gttctggggg tggtgccact cgtcatcgcc
     8521 tcgggtgcgg gcgcggcagg acggcgggcg atgggaatcg tgctcttcac cggtctgtcg
     8581 atcggcacgc tgttcacgct cttcatcgtg ccggccgtct atattctgat tggggccgat
     8641 cacgcgaagg accggctgaa gcggtccaag aaggactcgc gagagggcca ttccgcaccg
     8701 cccgaggcct tggcctgatc gaaggttgca gcggcctgcc gttatcgggt gccaccgcac
     8761 gggcctgcgc ggtgccgctg acatccgcgc gatcacgccg gtttacctgg ggttgcggtt
     8821 tggcggagag gcttggttgg ggcgacttgc gcccaagtcg ggcccgtcgg cgtacacagg
     8881 atttgcggca ccggcacctg ccaccgttgc ccactcgcgc acgcgccagc gacttaccag
     8941 gccattgatg acatacggat cggtccgggc aaactcctcc gccacctcgc gcgcctcgcc
     9001 cgcgaacagc agcacggcac catcgagcgg atcgttgagg gcaccgccca gcagcagctc
     9061 gccgcgacgg tgggccgccc aggccttcgc cagatgcgcg tggcgaaatt cgggccgccg
     9121 tgcgaggtaa ttctcgctca cttcgtagaa cagcaggaag tgtttcatga cggctagcgc
     9181 atcacgggca tggtgaactc ggcatcgctt cccggagtgc gcggccaacg ctgggtcacg
     9241 cacttcaatc tcgtgtagaa gcggacgcct tcctcgccat aaatgtggtg gtctccgaac
     9301 agcgagttgc gccagccgcc aaagctgtag aaggccacgg ggaccgggat gggaacattc
     9361 acgccgacca tgcccacctc gatcgcagtc gcgaacgcgc gggcggcatc accgctgcgc
     9421 gtgaacactg acgtcccgtt ggcgtaagtg tgcgagttga tcagccggat ggccgcatcg
     9481 gcgtggggaa cccttacgat gaccagcacc ggtccgaaga tttcttcgcg gtagattttc
     9541 atgtcgggcc gcacgcgatc gaacagcgac cccgcgagga aaaatccctc ctgcgctccg
     9601 ggcagtgcag tcccccgacc gtccagcacc aggctcgccc cttcggcaac gcccgcttcg
     9661 atgtagccgc gtacccgatc gagatgctca cgtgtgacca gcgggcccat ttccgtctgc
     9721 tcatcatcgg gggccccgat gcgcaggccc ttgatgcgcg ggacgagttt ggccaccagg
     9781 cggtccgccg tctcatctcc tactgcgacg gcgaccgaga tcgccatgca tcgctcgcca
     9841 gcagatccga atgcggcacc gagtaatgca tcggtcacga ggtcgagctc agcatcgggc
     9901 atgacgacaa ggtgattctt ggcgccgcca agggcctgta cacgcttacc gtgcgaggtt
     9961 ccctcgagat agatgtgtcg ggcgatcggt gtcgagccga cgaagctgat ggccgcaaca
    10021 tccgggtgat acagcagcgc atcgaccgcc tccttgtcac cctggatcac gttgaagacc
    10081 ccgtccggca gccccgcctc cttcagtaac tcggccagtc gtaccgaagt ggacgggtcc
    10141 cgttctgacg gtttcaacac gaaggtgttc ccacaggcga tggcaatggg aaacatccac
    10201 atgggcacca tcgccgggaa gttgaaaggc gtgatcccgg cgaccactcc cagtggttga
    10261 cgcaggctga aggtgtccac gccggtgccg acgttctcac tgaattcacc cttgagcagg
    10321 tgcggaatgc cgcaggcaaa ctcgacgatc tcgaggccgc gcgccagctc accatgcgca
    10381 tcgctcacgg tcttgccatg ctcgcgggag atcaggtgcg caatctcacc cgcagcgtgc
    10441 tccaccagct ccttgaatcg aaacatgacc cgcgcgcgcc gcggtgcggg cgtcgaggcc
    10501 caacctcgcg ctgcttcgcg cgccgctgcc actgcggcgt tcacttcctc cttggaggcg
    10561 aaagcgaccc gggcggcgac cgcaccccgc gcggggttga agacatttcc ggtccgtgcc
    10621 gggttgctcg ctgcgcggcg gccgccaatc cagtgggaga cggtaggcag ctcagaggag
    10681 agttcagtgg ggttgcgcat catggcgtcc agatcgtgca gatcctgaga tggtggaacg
    10741 ttattgtgca gcagctgccg cgccgtttcg agcgtcaacg agacgggtcg gcatggcagt
    10801 ggtcagaggg ccctcatcgc tttaggatgc acctcggttc gcgtggctcc ggggggaagc
    10861 cgatcatgaa caagcgcagc gtcagccgca gagccttcgt gggatttgcc ggtgccggca
    10921 tcgcgacgct gatacgatcg ccggccgcgc tggcggcctt gcaaggtgag ccgcgcgacc
    10981 cggacctgat cgttttcaac gccaaggtct acaccgtcga tgcgaaacgg cccaaagccg
    11041 aagccttcgc cgtcaaggcg ggaaggttca ttgccgtggg ctcgaccgaa gacatcaaat
    11101 cgctggccgg caagtcgacc cagagctttg atgcgcagca gatgacgatc gtcccgggat
    11161 tcattgattg ccacaatcat gccactggca acatgctcct ctacgatgtg ctggtcggta
    11221 atccctacga ggttgaattc gtcaccatct ccagcatcgt ggagaagctc agggctcgtg
    11281 cgcagaagac gccagccggt ttctgggtgg agggatattt ctacgatgac acgaaggtga
    11341 aggacgggcg ccagatcaac attcatgatc tggaccaggt ctcgcggcag catccggtcg
    11401 gcgtcgagca ccgcggtggg cacacacatt tcttcaacag caaggcgttc gagctcgccg
    11461 gcgtcacccg agcgactccg gatccgcccg ggggcacctt cgatcggggg cccgatggcg
    11521 agctcaatgg ccgcgtgacc gataatgcgc tcgcggcgtt cgagaaagtg ggcaagcgac
    11581 ccgctttcag tgaggccgag aggcggcagc gcgatcggga cggcctggct catatctcga
    11641 agcagttcgt tcgttacggg ctgaccagtg tgcatcacga ggagggcgac ttcgccgctc
    11701 tacaggagat tcgagcgcgc gggcagctgc tgcatcgggt gagttacgaa atggacgacg
    11761 gcatgctgga agcgatgctg gccaacggaa tcgagaccgg ctttggcgat gaatggctgc
    11821 gactcggggc cacttcggag catcaggtgg acggctcgtt ttccgagcgc acgatggcgc
    11881 tcagcgtgcc gtaccccgga tcaaaatcgg ggtaccgcgg taatctcacg gagacgcagg
    11941 cgactctcga cggctggatc gagcgtgtct ggcgtgcggg tatccagccg aactgtcacg
    12001 cgaatggcga tgtggcgatc gatgcggtgc tgcgctcgct gcagcgggcg caagagcgct
    12061 tcccgcgtgc ggatgtccga ccgaaaatca cacattgcac actgatcaat gatgatctgc
    12121 tcaagcggat gaaagtcctc ggtgtcgtac cgaccccctt caccagctac gcgtattaca
    12181 actccgacaa gttccacttt tacggtgagg ccctcatgcg gcactgcatg gcctaccgct
    12241 cgttcatcga cagcggaatc acggccgcgg ccggatcgga ctttccgcca ggcccgttcg
    12301 cacccttgat gggtattcag ggcatggtga ctcggaccgg ctggaacggt gaaacgtggg
    12361 gcgccaatca acgcgtcacg gtcgccgaag cactgcagat caacacactc aatggcgctt
    12421 acgcctcgca cgaggagaag agcaaaggct ccatcactcc cggcaagctt gccgatttcg
    12481 tggttctggc cgaggacctc aacacggtcc ctcaggatca catcaaggac gtgaaagtat
    12541 tccgtacggt cactgagggt gccacggtgt atcaggcctg agttccgacc ggaaatctac
    12601 gagcgcgccc gcgcggacat cgactctcgg cacgccgctt tcgcgaacga gcgcactcca
    12661 gcgcctgcgc tcggactcga tgaaggcggc aaagctctcc ggcgtcgttg ctccgacttc
    12721 accaccttcc tcggcgatct ctgtcttgat gtcatcagat cgcaagactt cttggatcat
    12781 gcgattcaat ctcgagacaa tcgccggcgg tgttccagcg ggcgcgagga gaccggtcca
    12841 gatgcgaagc tcgtaatcgg tgacaccgac ctctgcaaag gttggtactc gtggcaagct
    12901 cgcgagacga ttcgcggcag ccacggccag cgggttctgc agacccgagc gaatccccgg
    12961 catcgcgccc ttgcacggat cgaacatcaa gcccgcctgg cccgcaagaa ccgccttacg
    13021 ggccggtccg gcgccgtcat aggggatgtg atgcaggtcg atattcagcc gagccttgaa
    13081 cagctctccc gcgacctcct cggggctgcc gatgccgttg gatgcgtacg agagactgcc
    13141 gggccgagcg cgggccaggt cgatgagctc ccttgccgag acaactggaa ccgacgccgc
    13201 cgctaccagc agcatgggcg aggactccag cagcgcgatc ggcacaaagt ctcgctcagg
    13261 atcgaacggc agggatgtgt acgtgtatcg atgcaccacc agcggcccaa tcgagcctag
    13321 cagcaacgtg tacccatcgg gcggagcatg agccaccaat gcggtgccct cattgccgcc
    13381 cttgcgcggc acgttgtcga cgatgatcgg ctggcccagt cgctcggcta accccgccgc
    13441 cagcaggcgt ccccaatggt ccgccccccc gcccaccggg tacggtacga ccaggtggac
    13501 ggggtgatcg gggtagggat gcgcagctgc tgacgcttgc gcgaagccca gcgcgttgat
    13561 cgcaagcacc cccgctttga gcagcagatc gcacgatgac ttgagaatgg ccatttgttt
    13621 gctccgcccg cagcagcctc gcccatgcct acagtgaaat tggacgcctt ggttcgagga
    13681 ccagtggcat ttcccggcgt cattccttcg atcggtcgcc gtcatcgtca ccatcatggt
    13741 ctccatcacc gtgtgcgcgt cgcagtgtgt gctcatacac gtaggcgccc acccgccgcc
    13801 cctgttcgcc cccggcgacc tgatcgaagc ggaaatggat acccccgaag acacgcgcgt
    13861 cattgatgtc gtgggttatc tgctcgaagg tttcgtattg cagagtcaca cccggcaccg
    13921 tggagttagt cagcgtgatc atgtgatggt ctctgccata gattcgctcg agtaccttgc
    13981 gtgccgcacc actcaggctg gcgtgtgccg atccataggc cgggaaacag ggggccttga
    14041 tgtagggcac ccagctcgta ttaccgctcg catggatcgc ggtttccggc cgccagaaat
    14101 gatcgaggta cttggtgtcg aacaccgatc ccgccgcatc gctgatggcc atgtccatga
    14161 gcgccaatgt ccgagcgttc tcggacaacg aacgcccacg ctcgacagcc acctgcgcgg
    14221 cggctgagtt ccagacggtt accgggggtg tggctgcgta gaatcgagcc acgtccgcgc
    14281 gatcctgcgg ccggaatgag ctgtccgcgc tacccacgcg ctgcacttcg tcgaagtcga
    14341 tcgcataccg catgctggtc agcgccggct gtggggccga gcgaaactga tcggtactca
    14401 cgatcgcgaa cggctgcaaa tgaggccatt gcagcagggt gccgcccgcg ggcggacagc
    14461 tcggcgtcgt ttgccattga ccggggtcac tggagcccgg aacgtagaac tgcatcggca
    14521 gcgacccgtc gctgctgcgc agctggatga acgcggcggc ggccgcctca cctaccccga
    14581 tcccatcctg cttcgcgctg ccgtcgggaa tgcgggcaag cgatacgccc tccgccgaat
    14641 ccagagcgcc ctggttgatg ggaaagtagt acaggagcac gtcgtgtgcc gcggcgatgg
    14701 cggcggcttc cggcgatgct ccggacggcg cggtgagtgt gccaaggtac ggatcgaagt
    14761 gcttctcgat cgtgttcacc gcttcgaaca cggccagctg ggtaattgcc gcggatcggg
    14821 cctgaaagaa cggattctga ctggccgtcg tactgaccat gatcgcgttc cactcgagca
    14881 ccgggtcggt ctgcgaaagc gcgacggtcg agaccagcgc gccgcacagc gcgccgagca
    14941 gcccaattga agttctcatt tgaacaactc cccatgaaag ctacatggaa ggtagctctt
    15001 acctacccag acacgggcca aggtcagaac ccgacatctt tggttggtcc gcttgcacaa
    15061 aagcgctatc ttcagcccac gggatcgagc ccgatgcaag ggcgtgcgat gggtcagctc
    15121 accgatctcc tcggacgggt gcaaggcggc gacgccgagg cgcgtgccgc gttatttgct
    15181 gccgcctaca ccgcattgca cgatctggcc caggcgcgcc tgcggcgcgg gggacgccac
    15241 acccttctcg acaccaccgg cctcgtgcac gagtcgtatc tgcgcttcgc gcgcgccggc
    15301 gaacttcgag ccgaagatcg cagggctttc ttcgcctacg cctcgcaggt gatgcgttca
    15361 ataatcctca acagcgtacg cgaacgggtc gcactcaagc gcggtgctgg gcagtctgca
    15421 caggcatcca acccggagga atcgcccgat ctcgtgagcg aagagcgggt ggtgttggaa
    15481 gtgcatgagg cgctgaagga cttggaaaag gcggatcctc gtctggcgca agtggccgag
    15541 atgcgctact tcgggggtta cagtgagaag gaaattgccg aaacgctgga ggttaccgaa
    15601 cgcaccgttc agcgcgactg ggaaaaggcg cgcctcattc ttgcggcggc gttacgctga
    15661 gtgatgtggg ctccgattgc tgatcgatcc gtccacgcag ggggtgcatg ggactctcca
    15721 ttccccagat gagccggatg agccgcctcc tcgatgaggc gctgccgctc gatgcggacg
    15781 ggcggcgcgt ctggctcgcg gctttgccag cggagcagcg ggacctcgca cagtccttgc
    15841 gcgatgcgtt actgcccggg gatgtcgagc gcgcgcagat cgagaagctg gcgtcgctac
    15901 cgtcactcgg cgccgcgagc caggcggaag cgccccccac gagcggcctg cacaccggcg
    15961 accgcgttgg cccgtacgag ctgattcggc cgctgggcac gggcgggatg gccgaagtgt
    16021 ggctagcgcg gcgcgccgat ggcgccttcc ggcgcgaagt ggcgctgaag ttgcccatgt
    16081 cgagttgtcc gcggcccgat ctcgagcagc gcttcgcccg cgagcgcgac atcctggcga
    16141 gccttgagca tccacatatc gctcgtttct acgatgccgg agtgggcagc ggcgggctgc
    16201 cttatttatc catggagtac gttgagggcc ggcccttgac cgaatggtgt gacgcgcatc
    16261 gcctcgggat ccggccccgg ctcgagttgt ttctgcagat gctcgaagcg gtgcaatacg
    16321 cgcacgagaa gcaggtcatt catcgggatc tcaagccctc caacgtactg gtgaccgagt
    16381 caggccagat tcggttgctc gacttcggcg tggcgcgact cctgcaagcg gacgagcccc
    16441 gcaccgagct cacccacgtc catggtcggg cgctgactcc cgactatgcc agcccagagc
    16501 tgctgcgggg cgagtcggtg gatgctcgcg gcgacgtcta ttcgctgggt gttctgctgt
    16561 acgagctgct aaccggcgtc cggccttacc gactgacaca ggctgcttcg attggcttgc
    16621 tcgagcatgc gattgtcacg gtgcaggtga aaaagcccag cactcagctc gaggcgaacg
    16681 ccgttgtctc ccgggacacg acgcctgaaa aatgggctcg gcaactgcga ggcgatctgg
    16741 atgcgattgc cctcaaagcg ctcgccaagg agccggcggg acgctacccg agcgctgccg
    16801 cgctgatgga ggacttgagg cgatacctca acgccaggcc ggtccaggct ctcccagctc
    16861 gattgacgga tcggctgcgc aaattcctgc ggcgtaatcg ggtgacggtg ggggtaacgg
    16921 tgacggcggt cgccatcgta gttgccgcag tcacgcttgc agtggttagg caaccggggc
    16981 agggtcccag ccgactatgg ctcgatcccc tggcgcgggc gcgggtcctc cgactcaccg
    17041 attttgccgg taccgagcag gccgccgcca tctcgcgtga tggaaggttt gcagcgtttc
    17101 tggcggcccg caatggacct ctcgatctgt ggttgactga aatcgggacc aaccattatc
    17161 gcaacctgac tgagggaagg atttctcaac tccgaaatcc agaaattcgt tcggtgggct
    17221 tctcgcccga cggctcactg gtgacctttt gggcacgcaa tggcgacggc tcgcgggccc
    17281 aggacatcaa cgtcatggcc gtcccaacgg cggggggtgc ggtgcagcct tacttgccgg
    17341 aggccgccga gctcgactgg tccgccgacg ctagacaagt ggtcttccac acgacggcac
    17401 ccggggatcc gctattcgtg cgcgccgcaa cggaggcgaa ggcgcatcgg atttacgtcg
    17461 atgcgccggg cgttcactgc cacttcctca cctggtcccc agacggagag ttcatttatt
    17521 tcgtccgcgg cgatccgccc tctgccgact gggatatctg gcggctgcga ccctccggag
    17581 cgggactgga gcggctcacc tctcagcacg cttgggtgac ctatccagtg ctgctcgatg
    17641 cgcgaacact tctgtacctg gcgaccgacg ccgacgggtc gggcccgtgg ttgtatgtcc
    17701 tcgatttggt atcgaagcgc tcgcgccgtg tcagcgtcgg cttggagcga tatacgtccc
    17761 tctctgcgaa cgcggatcgc acccgcatct tggcgacggt cgccgactcc cgctcagagt
    17821 tatggcgcgc tgcgatcggc aacagcggat cgccgcaggc gacggccgaa cggatcgcgg
    17881 gagtgtccca gagtgcttcc gcgccacgca tcgggccagg ttacatcgcc tatgtgtcga
    17941 gcctcggcgc gcgacggggg atctggaagt acgcgaacag gacggccacc gagctttgga
    18001 gcgatgcgac cgctgatcgg gttggcgcgc tcagcatctc acccgatggg cgccgcattg
    18061 ccttcgtcgt ggacagagga gggacgacgc agctctacgc cgttgacagt gacggtcgga
    18121 accccagact cgtcgctcaa acgcttaccc tgcgagggga tctcgcctgg gctccggatt
    18181 ctcagtccat cctgagcgca atcgagagtg acggcgaacc gaggctcgcc cggatcttcc
    18241 tcgactcctc cccaccgcag ccgatggtct cggagtattc cgtcgatccc gcatggtcac
    18301 cggatggaaa gtacttcgtc tattccggtg cgcaggtggc cacgatattt cctctgcgag
    18361 cttcggcgcc tgacggaaga ccttacggca tgcctggtat gatcctgaca atcggggcgc
    18421 gacgggttgc gttcgcacgt aattccggtt
//
LOCUS       DASRHN010000432         4998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_164557, whole genome shotgun sequence.
ACCESSION   DASRHN010000432 DASRHN010000000
VERSION     DASRHN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164557"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /gene="hrpB"
                     /locus_tag="VFB37_17580"
     CDS             <1..2084
                     /gene="hrpB"
                     /locus_tag="VFB37_17580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HZO24308.1"
                     /translation="AVAQMLDDAPIITAAGKLFPVTTRYVGRSAPVLPAAAGPGTRDD
                     PAQLIASTVMRALREETGDLLVFLPGAREIRRVQGLLEAAHAGPDVRVLPLFGELSAD
                     EQETALAPAGPGMRKVVLATNIAETSLTIPGVRVVIDSGLVRRSLFDPVTGMSRLETQ
                     RISRASADQRQGRAGRLGPGVCYRIWSEPAQRSLAPFTAPEIVDADLVPLALELAHWG
                     ASNPAELCWLDEPPGAMLASARVLLETLGALDAAGRITAHGRRMAQVAVHPRLAHMLL
                     RALDLQALPLAADLAALLSARDLLSGADGARDADIRTRLDLFRGAEPARGAGWGAWRR
                     AHQAAGTLRRQVLAVCDRRGEDAGSSRAARGPADPDPGVLLAFAYPDRIGRRRPGAEN
                     RYTLANGRGAHFAEAQSLARQELIVALDLDDRERDARILLAAPVQRADIETHLADRLE
                     RGQSVEWNPREEAVLARRYVRLGAIVLEDSPLPEVPRAAAREAMLEGIRQLGIGALPW
                     TAEARNLQARIGFVQRNRNPAESWPVVSDARLAESLQDWLSAWLDAITRREQLSRLSM
                     TDILRGLLSWEQKRQLEELAPSHLSVPSGSRIPIDYVDDNAPAVSVRLQEVFGLQSTP
                     HICEGRVPITFKLLSPARRPVQVTRDLASFWRGAYAQVRKDLRGRYPRHYWPENPLEA
                     EPTRGVLRRRT"
     gene            complement(2021..4882)
                     /gene="ligD"
                     /locus_tag="VFB37_17585"
     CDS             complement(2021..4882)
                     /gene="ligD"
                     /locus_tag="VFB37_17585"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HZO24309.1"
                     /translation="MASRKLSKYRSKRNFSRTAEPSGDAAGVSRARRLRYVIQKHAAR
                     RLHYDLRLELDGVFKSWAVTRGPSVDPAEKRLAVEVEDHPLEYGDFEGTIPKGEYGGG
                     TVQLWDRGYWSPEGGQSAAEAIRAGNLKFTLDGERLQGSWVLVRMKNDRYGSGKRTNW
                     LLIKHRDEYARPADHDAILAEDRSVASGRPMAAIEAGKGRAPRPFMLADKPGHRTGAA
                     SVWHSNREADPAPKTKAAAPARKSARSSTRARKGALPQFVPPQLCKLVDRPPGGEGWG
                     HEIKLDGYRLQLRVQQARATLKTRKGLDWTRKFGAVPSAAQTLPDCLIDGEIVAVNER
                     GIPDFAALQAALAQGEGDKLVFFAFDLLYLEGEDLRDQPLAARKQALRTLLESQPKAL
                     QTHIRYLEHFETGGDAVLRSACRMELEGVVSKRLDAPYHSDRSGSWVKSKCRAGHEVV
                     IGGWSSDAGALRSLIVGVYRDGQLVPVGRVGTGFGREKLAPLMKRLKAVATADSPFHG
                     KVPVPGGRKINWVKPELVAEIEFAGWTEGGNVRQAAFKGLREDKPAREVRAEKPTLLP
                     LDEPQSMPSDTRTRPASRTNTSGHRRSGKSAQGSASKRSKPSSPAQAGAIRSAGSASG
                     SAGSATDNVLGVTISKPDKALWPDGGDGDPVTKLDLARYLASVGEWMLPHLSGRPCSI
                     IRAPDGIAGQRFFQRHAMPGMSNLVDLLRVPGDSKPYVQINRVEGLIAVAQTAGLELH
                     PGGCIPGKPEIPGRLVFDLDPAPDVEFEAVIEGALELRQRLSAVGLESFCKTTGGKGL
                     HVVTPLRAARGSELTWPLAKAFAREICSKMAEQAPDRYLLNMARSERQGRIFLEYLRN
                     DRLSTAVAPLSPRARPGAPVSMPLNWQQVRSGLDPMRFTIRTAPALLERGRPWREYSE
                     REGSLRAAIEQLARSGQRTQVRRRSTPRVGSASRGFSGQ"
BASE COUNT          757 a         1640 c         1701 g          900 t
ORIGIN      
        1 ccgcggtagc gcagatgctc gacgatgcac cgatcatcac cgcggcgggc aaactatttc
       61 ccgtcaccac gcggtacgtg gggcgcagcg caccggtgct gccggccgct gcaggtcccg
      121 gtacgcgcga cgacccagcg cagctgatcg catcgaccgt catgcgagcg ttgcgcgagg
      181 agacgggaga cttgctggta tttctgcccg gcgcgcgtga gattcgccgc gtgcagggcc
      241 tgctggaggc tgcgcatgcc ggtccggatg tgcgcgtgct gccgctgttt ggtgagctgt
      301 ccgcggacga gcaggagacc gcgctggcac cggcaggacc cggcatgcgc aaagtcgtgc
      361 tcgccaccaa catcgcggaa accagcctga cgatcccagg cgtgcgggtg gtcatcgatt
      421 cggggctggt ccgccgctcg ctgttcgatc cggtcaccgg catgagcaga ctcgagacac
      481 aacggatttc gcgtgcctcg gcggatcagc gccagggtcg ggccggacgg ctcgggccag
      541 gcgtctgcta tcggatctgg agcgagcccg cgcaacgaag tctcgctccc ttcactgcgc
      601 ccgagatcgt ggacgccgat ctcgtccccc ttgcactcga gctcgcacac tggggagcaa
      661 gcaatccggc tgagttgtgc tggctggatg agccgcccgg cgcaatgctc gcgagcgccc
      721 gggtgctgct cgagactctg ggcgcactcg atgctgccgg gcgcatcacg gcgcatggac
      781 gtcggatggc gcaggtggct gtgcaccctc ggctcgccca catgctgctc cgtgcgctgg
      841 accttcaggc gctgccactc gcggccgacc tcgcggcgct gctttccgca cgggatctgt
      901 tgagcggcgc ggacggggcc cgagatgccg acatacggac tcggcttgac ctgttccggg
      961 gggcggagcc tgcgagaggg gcgggttggg gggcgtggcg gcgtgctcat caggcggccg
     1021 gcactttgcg ccgccaggtg ttggcggtgt gcgatcgccg aggggaggac gcggggtcgt
     1081 cgcgggcggc gcgcggtccc gccgatcccg acccgggagt attactggcc tttgcatacc
     1141 ccgatcgcat cggccggcga cggcccggtg cggagaatcg gtacacgctt gctaatggcc
     1201 gtggcgcaca ttttgccgag gcgcagagtc tcgcccggca ggagctcatc gtcgcgctcg
     1261 acctggatga tcgcgagcgc gatgcccgga ttctgcttgc cgcacctgtg caacgggcgg
     1321 acatcgaaac tcacctggcc gaccggctcg agcggggaca gtcggtcgaa tggaatcctc
     1381 gcgaggaggc tgtgctcgcc cgccgctacg tgcgcctggg cgccatcgtg ctggaggaca
     1441 gcccattgcc ggaggttccg cgtgctgcgg cccgcgaagc catgctggaa ggcatccgcc
     1501 agctcggcat cggtgcgcta ccctggacgg ccgaagcgcg caacctgcag gcaaggatcg
     1561 ggttcgtaca gcgcaaccgc aatcctgcgg agtcctggcc agtggttagc gacgctcggc
     1621 ttgcagaatc cctgcaggac tggctctctg cctggctcga tgccatcaca cgccgcgagc
     1681 aactctcgcg tctgtcgatg acggacatac tgcgcgggct cttgtcgtgg gagcagaagc
     1741 gtcagctcga ggagcttgcg ccttcacatc tgtccgtgcc gagcggatcg cgcatcccga
     1801 ttgactatgt cgacgacaac gctccggcgg tatcggtgcg actgcaggag gtttttgggc
     1861 tgcagtccac acctcacatc tgcgagggcc gtgtgccgat cactttcaag ctgctctctc
     1921 ccgcgcggcg gccggtgcag gtcactcgtg acctcgcgag cttctggcgt ggagcctacg
     1981 cgcaggtgcg caaggatctg cgcggccgct acccgcgcca ctattggccc gagaatcctc
     2041 tcgaggccga gccgacgcgc ggggtactcc ggcgacggac ctgagtgcgc tggccgcttc
     2101 gagcgagttg ctcgatggcg gcgcgcaggg atccttcgcg ctcgctgtat tcgcgccagg
     2161 gtcggccgcg ctcgagaaga gcgggtgcgg tgcggatcgt gaagcgcatc ggatcgagac
     2221 cggagcgcac ctgctgccag ttgagcggca tggataccgg cgcaccgggc cgtgcgcgcg
     2281 gtgaaagggg ggcgactgcg gtcgacaggc ggtcattgcg cagatattcc aggaagatgc
     2341 ggccctggcg ctcgctgcgc gccatgttca gcaggtagcg atcgggcgcc tgctcggcca
     2401 tcttgctgca gatctcacgc gcgaaggctt tcgccagcgg ccaggtgagc tcgctgccgc
     2461 gggccgctct gaggggcgtg acgacatgca accctttgcc gccggtggtc ttgcaaaagc
     2521 tttcaaggcc gaccgctgag agcctttgac gcagctcgag cgcgccttcg atgaccgcct
     2581 cgaattcgac atccggtgcg gggtccagat cgaagacgag ccgtccgggg atttcgggtt
     2641 tgcccggaat gcaccctccg ggatgcaact ccagaccggc ggtctgggcg acggctatga
     2701 ggccttcgac ccggttgatc tggacataag gcttgctgtc gccgggcact cggagcaggt
     2761 cgaccagatt cgacatgcca ggcatggcgt gtcgctggaa gaagcgttgg cctgcgatgc
     2821 cgtcgggggc gcgaatgatg gagcaggggc gcccgctcaa gtgcggcagc atccactcgc
     2881 cgaccgaggc caggtaacgg gccagatcga gcttggtgac cggatcgccg tcaccgccat
     2941 ccggccagag cgccttgtcg ggtttggaga tcgtcacgcc gagcacgttg tctgtggccg
     3001 agcctgcaga ccctgaggcg gatccggcgg atcggatcgc gccagcctgc gcggggctgg
     3061 agggtttgct gcgcttgctt gcagagccct gcgcggattt tccagatctc cggtgccctg
     3121 aggtgttggt gcgggaggcc ggccgggtac gagtatcact gggcatgctt tgcggctcat
     3181 cgagtggcag cagcgtcggc ttctcagcac gaacctccct ggcgggctta tcctcgcgca
     3241 ggcctttgaa ggcggcctgg cgcacgttgc cgccttccgt ccagccggcg aactcgatct
     3301 ccgcgaccag ctcgggcttt acccagttga tctttctgcc gcccggcacc ggcaccttgc
     3361 cgtgaaacgg actgtcggcg gtggcgacgg ctttgagcct tttcatcaga ggagcgagct
     3421 tctcgcggcc gaagccggtt ccaactcggc ccaccggcac gagctgtcca tcgcgataga
     3481 ctccgacgat cagcgagcgc agcgcccctg cgtccgatga ccatcctccg atcacgactt
     3541 catgacccgc ccgacacttg gatttgaccc agctcccgct gcgatcggag tgatacggcg
     3601 catctagcct cttggacacg acgccctcga gctccatgcg gcacgccgag cgcagaactg
     3661 catcaccacc ggtctcgaaa tgctccagat agcggatgtg ggtctgcagc gcctttggct
     3721 gggattccag cagcgtgcgc agcgcctgct tgcgcgcggc gagtggctga tcccgcaggt
     3781 cctccccctc gagatagagc agatcgaagg cgaagaatac cagcttgtcg ccttcgccct
     3841 gcgcgagtgc cgcctgtagc gcggcaaaat cggggatgcc tctttcattg acggcgacga
     3901 tctcgccatc gatcagacag tcggggagag tctgcgcagc gctcggcacc gcgccaaact
     3961 tgcgagtcca gtccagaccc ttgcgagtct tcagcgtcgc ccgcgcctgc tgtacacgca
     4021 gctgcaagcg gtagccgtcg agcttgatct cgtgccccca gccctcgccc cccggaggtc
     4081 gatcgaccag cttgcacagc tgtggcggta cgaactgggg cagagcgcct ttgcgcgcac
     4141 gggttgagga tcgtgcgctc ttgcgagccg gcgcggccgc tttcgttttg ggagccgggt
     4201 cggcctccct gttggaatgc catacagagg cggcccctgt gcggtggccc ggtttgtcag
     4261 ccagcatgaa gggcctcggt gcgcggcctt tgccggcttc gatcgcggcc atgggccggc
     4321 cggaggccac ggagcggtct tccgcgagga ttgcatcgtg atccgcgggc cgggcgtact
     4381 catcacgatg cttgatcaat agccagttgg ttcgcttgcc gctcccatag cggtcgtttt
     4441 tcatgcgcac cagcacccag ctgccctgga ggcgctcacc gtccagggtg aacttcaggt
     4501 tgccggctcg gatcgcctcg gcagctgact gccccccctc ggggctccag tatccgcggt
     4561 cccaaagctg caccgtgccg ccgccgtact ctcctttggg aatggtgccc tcgaagtcgc
     4621 catattcgag gggatggtct tccacctcca ccgccaggcg tttctcggca ggatccacag
     4681 agggaccgcg cgtcacggcc caggacttga acactccgtc aagctcgagt ctcagatcgt
     4741 agtgcagccg tctggccgcg tgcttctgaa tgacatagcg aagcctgcgc gcgcgagaga
     4801 cacctgcagc gtccccgctc ggttcggccg tgcggctgaa attgcgcttc gagcggtact
     4861 tggagagttt gcggctggcc atgacgatcc cggagtgcgc gttgcggggc gacgaggcgc
     4921 aagagtcgcg cctgccgttc ccgtcatggc tctgacgctc gtgcgcccgc gcccttactt
     4981 cttgatcagg ccctccgc
//
LOCUS       DASRHN010000433         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1812035, whole genome shotgun sequence.
ACCESSION   DASRHN010000433 DASRHN010000000
VERSION     DASRHN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812035"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFB37_17590"
     CDS             <1..373
                     /locus_tag="VFB37_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24310.1"
                     /translation="ALHPVAGQGFNLGLRDAAMLAEVLAAARSATGARDDPGDAALLR
                     QFAEWRAGDRQGVVRFTDGLVRLFGSSRPGVGLLRNFGLLLFDLTPPAKGALARVSAG
                     FAGPSPRLARGLALRHTGGAP"
     gene            370..1608
                     /locus_tag="VFB37_17595"
     CDS             370..1608
                     /locus_tag="VFB37_17595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZO24311.1"
                     /translation="MTETVDVLVVGGGPVGACLAALLMRESAASSKPLSVMVLEPNRP
                     ALLEPGSAFDTRVVALSRASEHILAKAGAWSRIRGPRVNAYERMRIWHESISGTSADV
                     LVFDAAEAGEPDLGHIAESRLLQAALLDSYAESGGRIEPLGLRSVHVAEEQVNVRTEA
                     GAVNARLVVGADGVRSAVRDFVGLTADVSDYRQTAIVATIATERAHQNTAWQRFMRDG
                     TLALLPLADGSTSIVWSADDPLAAALLARPASDFEAELDRASDHALGATRLVSERSSF
                     ALKGLSAHHYVARRCALIGDAAHVVHPLAGQGVNLGLLDAAALAQLILEAARQREDPG
                     ALRVLRRYERWRKSEVAVMNAAIASFDRLLAHGTGPVSRIAQRGLGLVNRSAEIKRFF
                     IRRALGMSGELPRAAMTGGP"
     gene            1605..>2145
                     /locus_tag="VFB37_17600"
     CDS             1605..>2145
                     /locus_tag="VFB37_17600"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HZO24312.1"
                     /translation="MSLGALGLSIASEPQEPTLSPAQLIRATLTMGARTVGGWSKQEE
                     ALAAEVDASHCSLDRAIVEHLCERIRSGEDPLGDALISSRVRSDRRRTGTTYTPQEIV
                     GALLELVRRESNPERIVDPGAGSGRFLLQAARTFPAATLLGIELDPLAALIARANLAA
                     VGAAHRSRILNADYRRVDLR"
BASE COUNT          332 a          689 c          740 g          384 t
ORIGIN      
        1 ggctttgcac ccggttgcgg gtcagggctt caacctcggg ctgcgcgatg ccgccatgct
       61 ggcggaagtc ctcgcggcag cgcgcagcgc cacgggtgca agagatgacc caggtgatgc
      121 ggcgctgctg cgccagtttg cggagtggcg cgcgggggac cgccagggag ttgtgcgctt
      181 caccgacggt ctggtcaggc ttttcgggag ctcgcggccg ggcgtgggtc ttctacgcaa
      241 cttcggtctt ctgctctttg acctgacgcc gcccgccaag ggtgccctgg cacgagtgag
      301 tgccgggttt gctgggccga gcccgcgact cgcacgcgga ctggcactgc gacatacagg
      361 tggagcccca tgaccgaaac agtcgatgtg ctggtcgtgg ggggcgggcc ggtcggcgcg
      421 tgcctcgctg cattgctgat gcgtgaatcg gccgcatcat ccaaaccgct gagcgtcatg
      481 gttttggaac cgaacagacc ggcacttctc gagcccgggt ccgctttcga cactcgcgtc
      541 gtggcgcttt ctcgcgccag tgagcacatc ctcgcaaagg cgggagcgtg gtcacgtatc
      601 cggggaccgc gtgtgaacgc ctacgagcga atgcggatct ggcatgaaag tatctcgggc
      661 acttcagcgg acgtcctggt gttcgatgcc gccgaggccg gtgagcccga cctcggacat
      721 atcgccgaga gccggctgct gcaagcggcg cttctcgact catacgcgga gagcgggggt
      781 cgcatcgagc cgctcgggct gcgctcggtg cacgtggctg aggagcaggt caatgtaagg
      841 accgaggccg gcgcagtgaa tgcgcgactc gtcgtcggag ccgatggtgt ccgatcggcg
      901 gtacgcgatt ttgtcggtct cacggcggac gtgtccgatt atcggcagac ggctatcgtc
      961 gccacgatcg cgaccgagcg tgcgcatcaa aacaccgcgt ggcagcgatt catgcgtgac
     1021 ggcacgcttg cactgctgcc cctggcggat ggttccacct cgatcgtctg gtccgcggac
     1081 gatcccttgg cggccgcgct gcttgcgcgt ccagcatcgg atttcgaagc ggagctcgat
     1141 cgagcctccg accacgctct gggtgcaacc cggctggtga gcgagcgaag ctcgtttgcg
     1201 ctgaagggcc tgtctgcgca ccactatgtg gcccggcgct gcgccctgat cggggacgcg
     1261 gcgcacgttg tgcatccgct cgccggacag ggagtcaacc tcggacttct ggacgccgcg
     1321 gcactcgcgc aactgattct ggaggccgca cggcagcgcg aagatccggg ggcactgcgg
     1381 gttttgcggc gctacgagag gtggcgcaag agcgaagtcg ccgtcatgaa cgctgcgatc
     1441 gcctcattcg accgcctgct cgctcatggc acggggcctg tgtccaggat cgcgcagcgg
     1501 ggtctgggtt tggtcaatcg cagcgcggag atcaaacgtt tcttcatcag acgcgcgttg
     1561 ggcatgtcgg gggagttgcc gcgggccgcg atgacaggcg gcccatgagc ctcggagcac
     1621 tcgggctcag catcgcgagc gagccccagg agcccacact ttcccctgcc cagctgatcc
     1681 gtgcgaccct gaccatgggg gcccgcacgg tcggcggctg gtcaaagcag gaggaagcac
     1741 tagccgctga agtagacgca agccattgca gcctcgaccg ggcgattgtt gagcacctgt
     1801 gcgagcgcat acgctcgggt gaggacccgt tgggcgatgc cctcatttcc tcgcgggtgc
     1861 gctcggatcg gcgccgcacc ggcaccacct atacgccgca agagatcgta ggcgctctgc
     1921 tcgaactggt ccgccgggaa tccaaccccg agcgtatcgt ggaccccggt gcaggctctg
     1981 gccgctttct cctgcaggcc gcgcgaacgt ttccggccgc cacactcctg ggcatcgagc
     2041 tcgaccctct ggctgcattg atcgctcgcg ccaatctcgc cgcggtcggg gccgcgcatc
     2101 gctcccggat cctgaacgca gattatcgcc gcgtggatct gcgca
//
LOCUS       DASRHN010000434        15376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2461626, whole genome shotgun sequence.
ACCESSION   DASRHN010000434 DASRHN010000000
VERSION     DASRHN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15376
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461626"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VFB37_17605"
     CDS             <1..1598
                     /locus_tag="VFB37_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24313.1"
                     /translation="TTTYADPAVQAEIEKHYLAVSVDADSDPALTSRYGDWGWPATIV
                     LKADGTEIVKRRGYIPPAQMLSLLNAIVADPTPGPSIGAALQIADTGATSLGQKQRAA
                     LLGSFDNLYDETNAGWGSGHKYVDASSIEWSFAALDAGNQAAIRKIRHTLDANLNLID
                     PVWGGVYQYSDHIDWKSPHFEKLMSFQADDLRVYSDAYARWHRPSDLGAAQKIYGYMT
                     KFLSAPDGGFYVSQDADVSPKVTGHDFYSKDDAARRAIGMPRIDTHEYSRETGWAIRA
                     LCKYYDVTAEAQALERAKQAARWALSARGFPGGGFGHAAAKPEAGGPFLDDNVSMTQA
                     FVALYRSTGDREWLHDALATLDFMDAKLRDLRAGYVAAPPPSGGRGVFREPIRDSAQN
                     AELVSAASMLHHYTGAERPLQVAQHAMKYLSALAAADSDRLRPEILLADHELSVPPIH
                     ITVEGNKDDPAARALHAAALRYPSDYLQVDWWDRREGKLPDPAITYPVLPRAAAFACT
                     ANACSTPVFDPAELEGRVHAVLN"
     gene            1804..3252
                     /locus_tag="VFB37_17610"
     CDS             1804..3252
                     /locus_tag="VFB37_17610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO24314.1"
                     /translation="MLSILVCHSYFLRFDSKQVERGKPYPPLATLQAAALLREAGHEV
                     ALFDAMLAEGLAEYEDKLRAVRPQVVLLYEDNFNFLSKMCLAKMRSAACEMIASARRA
                     GARIIASGPDVTDAPEPYLQSGADFALLGEGLATLLELLPRLQSADGRPAEELSQGLS
                     GIATRDQGRVNRINGAKVLPTGTQQDEFAAWDLVDMEPYREMWLRTQGYFSLNMAASR
                     GCPFRCTWCAKPIWGNQYLQRSAAAVAREMVYLRRRFAPDHVWFADDIFGFRVDWVNE
                     FAAALTAGGGGVPFTIQTRADLVSERMAQALRAAGCQEAWLGAESGSQRVLDAMNKGT
                     TVNEIITARERLRAAGIRVGFFIQLGYLDEQLPDILATRALLETARPDDVGVSVSYPL
                     PGTKFHELVKAQLGDKTHWQDSNDLAMMFRGTYTSDFYRAVRNLLHEQVSLQRLDAEQ
                     HTAERFQVERRWQELLAREHQYRSPGAQAAAN"
     gene            3294..4532
                     /locus_tag="VFB37_17615"
     CDS             3294..4532
                     /locus_tag="VFB37_17615"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZO24315.1"
                     /translation="MTSTRFDIPPAGVVHTALRRTTETLARELAEPTATAPDWSEFEW
                     LIARATAAMHGVSSLLSGALRWQGPPGWASFLQNQRQHTLGRHARLLELLQRIDLHAR
                     RDGIALVALKGVELHALGLYAPGERPMGDIDLLVRAEDGPRAARLLVSMGFSESMPTW
                     KHRVFLPEHPSQPAALGEHVDNYLKVELHERVREALPLLPQEVTQWVFPPRPEAGLNR
                     YPSKASLMTHLLLHAAGAMASRSLRLLHLHDLAVLSERMAPQDWKELCAQSARDGGHW
                     WAAPPLWLTARYYGNAIPGRVLDELGAACPRLLMRFARRRALSDVSFSHLWISAFPGI
                     EWSQSAADLARYVMSRMRPSPEMLGLRRELVRTHLGSGASQWNRLSQARRLLRWLTSR
                     PARSETMHPVLMALAQARQI"
     gene            4529..5437
                     /locus_tag="VFB37_17620"
     CDS             4529..5437
                     /locus_tag="VFB37_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO24316.1"
                     /translation="MSAADITAPAASPPLVSSPAASTPAVPTGLLGDTPARDYTRKLQ
                     LFNKFAEPELRRAIDTLGLTPGMRVLDAGCGSGEALTWLAQQVGPSGEVLGIDLAIAH
                     INAARRFVAPRISVLQGDILSIPLAPASFDAIWCVNTINHLRDAIGGVRRLASLLRPG
                     GHLALGQSSLLADMYFAWDARLERLTNEAVRQYYRTRYGVGEGELAPVRSLVGLLRSA
                     PLQDVTSRTFVIERITPLKPADEAYLHEAIFLDTWGERLRPYLAAQDFSALSQLCDSG
                     ASHYALHRPDFHFLQTFTLAVGRVSS"
     gene            5434..6282
                     /locus_tag="VFB37_17625"
     CDS             5434..6282
                     /locus_tag="VFB37_17625"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO24317.1"
                     /translation="MSDSALSEHDRAAGTSPRLRDTRAAFDSVAADYDGPRGNNELIQ
                     DMRREMWRWLDATFAPGSRLLDLGCGTGLDAIRMARLGHRVTALDWSAQMVRRTAERA
                     ARENLSDRVQTLTIGAHELQRLPSSSEFDGAYSNLGPLNCVPDLTVVATECARLLKPG
                     GALVFTVIGRICPWEIMHYARRRQWARLRVRFARGVVPVGMNRHTIWTRYYQPRELYR
                     AFRPQFSLSLYRGLCVFAPPPYLTSVREKHPHWHERLWRLDRRCAGWPLLRAMGDHFL
                     IVLRRR"
     gene            6328..7140
                     /locus_tag="VFB37_17630"
     CDS             6328..7140
                     /locus_tag="VFB37_17630"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZO24318.1"
                     /translation="MLKALVRKTVGRRRIHSLQTLIRDSSIYTQEPAVTEPGAGKVVV
                     LAPHMDDETLGCGGTIARHAQAGAEVTVIFLTDGRYGGRSCPEATRPERERLQRELIE
                     TRKQEAQLAGQILGVRSLLFLDAEDTQLRRDQHVGARLRQILQQEKPDCVYLPFFLER
                     HVDHRAANEVLLAAVSGTALEFQCRGYEISTPLFPNCLVRIDTTLELKRQALSCYHSQ
                     LTDFDYVHAAIGLNAHRSMSLGGHCGRFAEAFHALPLREYRQFYHAVPVSLR"
     gene            7137..7952
                     /locus_tag="VFB37_17635"
     CDS             7137..7952
                     /locus_tag="VFB37_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO24319.1"
                     /translation="MTRLEPRSSTSGPVERDHVTGAEYVRQLSVRESDRRTRAAFQHL
                     ALGLVPPGGRVFDFGAGTGTDARFYAEHGLSVAAYDVDPRMQAFFAEYCQDFISSGQV
                     SLQGGGYRQFLAESAAPTGTDGAHLVTANFAPLNLIADLRELFERFHALTRPGGRVLA
                     SMLSPYFIGDLRYGWWWRNLGRLWREGSYAVQGTQGPVIRRRLSQLATQGLPYFTLQR
                     VFPGFAPTGSAHAAGLSPLDSGVDVHDGPGSAWRCAVGCLFMFLLYEKRSPAV"
     gene            complement(7957..8748)
                     /locus_tag="VFB37_17640"
     CDS             complement(7957..8748)
                     /locus_tag="VFB37_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24320.1"
                     /translation="MNGLRKAGKRAILGTLVSAEASLKAVGLARPSVLLLLGHMRSGS
                     TLLLHLLMTNPEIAALGERGVVYDSSADFARLALAARIRHGGPLRVLRYVADQVNHDE
                     LTPHASLLRHRRVKVLFLLRRPEPSLRSIVELYRAHYQQPWSTSRALEYYVGRVKSLT
                     RLGQSLSAQGNAALIRYESLTDAPQETLEALRVFLGLRRGFSPTYATYPFTGRHGDPG
                     PYIHAGRIIRERSATRIDLASSELERAAAAYDECTTALERFTLGV"
     gene            complement(8745..10880)
                     /locus_tag="VFB37_17645"
     CDS             complement(8745..10880)
                     /locus_tag="VFB37_17645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZO24321.1"
                     /translation="MSARNLPLALLPQAVSQAQAITPQSAGAVRGRSHAHAAIQYRAD
                     IDGLRGLAVGLVVAYHAFPLFRTGGFIGVDVFFVISGYVISQVILRALRAGTFSLSDF
                     YRRRVRRIVPALLLVLAACCLVGWYLMLPSELLWLGHSIKWCVPFLANLYFARTGGGY
                     FEPSAELNPLMHLWSLAVEEQFYIVWPLLLALAVKRGITMHFMGGVFALSLAFSIWSA
                     WHGTPHFYTPATRAWELAAGGILAAWQDLTPRAVIGRDAAKQAPTRLSLQAISVLGLM
                     LIVVGGVCWTTDRAIPGSWSLLPTAGAALLLATGPHTVSGGFLASRPMVLLGKISYPL
                     YLWHWPTLAFTLVVLGHHPSAGIASIEMGIAGLAAYGTYRLIESPIRYGRLGRKAVAP
                     LLAGLVLLTLAGMAFDRGWIPGRLSGSLVARWNQAVNDWHYSGETKDPQTGVGMLTVA
                     SQREHKTLFIGDSHIQQYRPRLQLLIDTYPDSARSAVLAGYPGCPPLPGIPRSWPDPK
                     CGGFFEFAIKQAGRADVDTVVFGAFWEHYFLGQYSPEGPVPHVYGLPASARVWLPLDS
                     PEARTALERFRKAISSLVSSGRRVFIVLSNPTSPQFDPRFPPGLRLALHLPRDLPRDV
                     TPQVDARAYEAFVAPLMDRLREIAAQTGARVLDPRSSLCAGMLCPAGDSGGFPLYLDS
                     NHLNGVNARERASFIDPTLFGPDIQPRRP"
     gene            complement(10867..12012)
                     /locus_tag="VFB37_17650"
     CDS             complement(10867..12012)
                     /locus_tag="VFB37_17650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO24322.1"
                     /translation="MKIALVVPGGVDRSGECRVIPALLALIARLSVRDEIHVFALAQE
                     PEPACWELLGARIHNVGAAHSRVRAIQAICAEHRSSPFHLVHSIWSGAPGLVAVAAAR
                     ILRLPSLIHVAGGELVALPEIGYGGRLGWKGRMREALVLRAATVVSAASDPMVAALAQ
                     LGISARRVPLGVDVKLWPPRAPVRRDPAKPARLLHVASLNRVKDQTTLLHALASLQHS
                     GVPFEMDIVGEDTLQGEIQAMAHQSGLAGSVRFHGFLPQRRLLPLLQDAHLLVMSSRH
                     EAGPLAVLEAAGQGVPTIGTAVGHIAEWAPDAAGAVPVGDWSALAGAITEMLADESLR
                     LRVAREALRRAVREDADFTARAFQALYTSVAAPGNAHRNVRGSGVCS"
     gene            complement(12009..13067)
                     /locus_tag="VFB37_17655"
     CDS             complement(12009..13067)
                     /locus_tag="VFB37_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24323.1"
                     /translation="MARNEHPGLAEPSADPFAERHRPRRREQFQLLGGRFRFETDSPQ
                     LLRIVREAYRGLPAHTLRNAGPRFSVRLALTGPHHRIDTQNEPPRIRAVSASGLLCGA
                     METASFVAIAPGQSSALVVVPREMLAFPYHVRYEMLEFAVYVLAARKQGLVPLHAACV
                     GYDGQGILLLGPSGSGKSTVSLHCLAEGLQFLAEDSVLVDPDGMLATGIANFLHARPD
                     SLKFLDARTRASLLGGSSIIRRRSGVEKLEIDLRRPRYRLASQPLRIRAVAFISARRA
                     GARALLAPLAQGRLMQRLAATQKYAAHQPGWARFVRQACRLPAFELRRGQHPHEAVEA
                     LMRVLQSSPDVVSKRAAR"
     gene            complement(13079..14887)
                     /locus_tag="VFB37_17660"
     CDS             complement(13079..14887)
                     /locus_tag="VFB37_17660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO24324.1"
                     /translation="MSLLSELWSILTPAQRRRMLAAQLVVLAMAFSTVTGIAAIAPFF
                     AVLGEPQLIDHNAVIHWLYLHLGLSSKRDFIVLLGSGFIAVVLVANLINAVGTLIMAH
                     LAMWIGNQLQTVLFNDYLSRPYSFHAATNSALLVKNIVYESVRVTHGILLNTLTLVAS
                     LVTAGFIIIACVLLNPTVTLATIAVLAAGYGLIYLSVRNRVLRCGQALSHLSTDQARV
                     VNEGFGAIKELTLRRARSYFSGRFERLSKELAAAAAHNQLIAQSPRYLMECVAAAGLV
                     GIALVLLGRSDGIGPWLGQLTFLAFAAYRLLPTLQQIFALIVKIRADRPALALIAPDL
                     RHAQTLRQASATAVPRPSLLDLQRRPRHEIRLEEVSFRYTGADSWALRGVSLCIPAQR
                     SVGIVGANGSGKSTLADLIAGLLVPSIGRILIDGAPLEDEQRAGWQAQVAYVPQSTFM
                     LDATIAENIALGTELADIDPARVREAARQAQLEELLGALPEGLLHKVGERGVRLSGGQ
                     RQRIGIARALYRQASVLLLDEATNALDGLTEKELMMTLRQLRGHYTSVLIAHRMSTVR
                     ACDLIIQLEHGRVIASGTYEELLRNSEAFRQMAGLR"
     gene            complement(14884..>15376)
                     /locus_tag="VFB37_17665"
     CDS             complement(14884..>15376)
                     /locus_tag="VFB37_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24325.1"
                     /translation="AQPSVVVSRELLERLGPFDESLLMCYDDELWLRLAAHSEIDGID
                     APLTLVRRHASHSGSDIIAWRDRRRVFEKALRTSGGGQLEPLLRKLRAQMSAGLARSQ
                     AAGGQRLDALTTLLTSLPHSLAYGGCWLGALRATAVAFAPQAARTLARHCRARLRALS
                     SLS"
BASE COUNT         2733 a         5053 c         5005 g         2585 t
ORIGIN      
        1 acacgacgac gtatgcagat ccggcggtgc aggcggagat cgagaagcat tacctggcgg
       61 tgagcgtaga tgccgacagc gatccggcgc tcaccagccg ctacggggac tggggctggc
      121 cggccaccat cgtgctcaag gccgatggca ccgagatcgt gaagcggcgc ggctacattc
      181 cgcccgccca gatgctttca ctgctcaacg cgatcgtggc cgatccgacc ccgggcccct
      241 ccatcggcgc ggcgctgcag attgccgaca ccggcgcgac cagcctcggt cagaagcagc
      301 gcgcagcact cctcggcagt ttcgacaacc tgtatgacga gaccaatgcc ggctggggca
      361 gcgggcacaa gtatgtggat gcgtcctcga tcgagtggtc gttcgcagcc ctcgatgccg
      421 gcaaccaggc ggcgatccgc aaaatccgcc acacgctcga tgccaacctc aatctgatcg
      481 accccgtatg gggcggtgtg taccagtact cggatcacat cgactggaag tcgccgcatt
      541 tcgagaagct gatgtcattc caggcggatg acttgcgcgt gtacagcgat gcctatgcgc
      601 gctggcatcg gccaagcgac ctgggtgctg cgcagaagat ctatgggtac atgacgaagt
      661 ttctgtccgc gcccgacggt ggtttctatg tctcgcagga cgcggacgtc tcgccgaagg
      721 tcacgggtca tgatttctac tcgaaggacg atgccgcacg gcgcgccatc ggcatgccgc
      781 gcatcgatac tcatgagtat tcccgcgaga ccggctgggc catccgggcg ctgtgcaagt
      841 actacgatgt caccgccgag gctcaggctc tggagcgcgc caaacaggcc gcccgctggg
      901 cactgagtgc ccgcggcttc cccggcggcg ggttcggcca cgctgcggcg aaaccggagg
      961 cgggaggccc gttcctcgat gacaacgtct cgatgactca ggcgttcgtc gccctgtatc
     1021 gctccaccgg cgatcgggaa tggctgcacg atgccctcgc cacactcgat ttcatggacg
     1081 cgaagctgcg cgatctccgc gccggctatg tcgccgcacc cccaccctcg ggcgggcgcg
     1141 gggtctttcg cgaaccgatt cgcgacagcg cacagaatgc ggagctcgtg agcgccgcca
     1201 gcatgctgca tcactacacg ggcgcggaac ggcccctgca ggtggctcag cacgcgatga
     1261 agtacctgag cgcactcgcc gcagccgaca gcgaccggct gcgccctgag atcctgctcg
     1321 ccgatcacga gctctccgtg ccgccgatcc acatcaccgt ggaaggcaac aaagacgacc
     1381 cggctgctcg ggccctgcat gctgccgccc ttcgctaccc gagcgactac ctgcaggtgg
     1441 actggtggga ccggcgcgaa ggcaagcttc ccgatcctgc catcacctat cccgtcctgc
     1501 cgcgggcggc cgcgttcgcg tgcaccgcca atgcctgctc cactcccgtg ttcgaccccg
     1561 ccgagctcga aggccgcgta cacgccgtcc tgaattgagg gactgcgttc ccgcgcagag
     1621 tctctgccga acgtcgcacc gcggaaactg cctcgggatg cttgaacccg gggaacgctt
     1681 cattgcactc acgcttcaag gcgggaattc ccgctcggcc ccgcctgcag tcgttccggg
     1741 aacgcggcgg gaggcccggg cgccggggaa cgccggactt ctccactgga gcggctgcgg
     1801 acaatgctct cgatcctggt ctgccactcg tattttctgc gattcgattc caagcaggtc
     1861 gagcgtggca agccttatcc cccgctcgcc acgctgcagg ccgctgccct gcttcgagag
     1921 gcgggacacg aggttgccct cttcgatgcg atgctggccg agggactcgc cgagtacgag
     1981 gacaagctgc gcgcggtgcg gccgcaggtg gtgctgctct atgaggacaa cttcaatttc
     2041 ctgagcaaga tgtgtctggc gaagatgcgc agcgccgctt gcgagatgat cgcgagcgcg
     2101 cggcgtgcgg gggcaaggat catcgcctcc ggaccggacg tgacggatgc tcccgagccc
     2161 tacctgcagt ccggcgccga ttttgccctg ctcggtgagg gactcgcgac gctcctcgag
     2221 ttgctgccgc gcctgcagag cgctgacggg cgcccagcgg aggagctcag ccagggcctg
     2281 tccggaatcg ccacccggga tcagggacga gtgaacagga tcaacggcgc caaggtgctt
     2341 cccaccggca cacagcagga cgagtttgct gcttgggatc tcgtggacat ggagccgtac
     2401 cgcgagatgt ggctgcggac acagggctat ttcagtctga acatggccgc ctcgcgcggc
     2461 tgtccgttcc gctgtacctg gtgcgcgaag ccgatctggg gaaatcagta tctgcagcgc
     2521 agcgccgcgg ccgtggcacg cgagatggtg tacctgcgcc gccggttcgc ccccgatcat
     2581 gtctggttcg cggacgatat cttcggattc cgggtggact gggtgaatga gttcgcggcg
     2641 gcgctgaccg ccggcggtgg cggcgttccc ttcacgatcc agacacgggc ggatctggtg
     2701 agcgagcgga tggcgcaggc gctgcgcgca gccggctgcc aggaagcctg gctgggcgca
     2761 gagagcggca gccagcgcgt gctggatgcg atgaacaagg ggacgaccgt gaacgagatc
     2821 atcacggcgc gcgagcggct cagggccgcc ggaatccgcg tcggattctt catccagctc
     2881 ggctacctcg atgagcagct acccgacatc ctcgctacac gtgcgctgct cgaaacggcg
     2941 cgcccggatg atgtcggagt cagcgtgtcc tacccgttgc ccggcacgaa attccacgag
     3001 ctcgtcaaag cccagttagg cgacaagacg cactggcagg acagcaatga cctcgcgatg
     3061 atgttccgag gcacctacac ctccgacttc tatcgcgcgg tgcgcaacct gctgcacgag
     3121 caggtctcct tgcagaggtt ggatgccgag cagcacacgg ccgagcgctt ccaggtggag
     3181 cgccgctggc aggagttgct ggctcgtgag catcagtatc gctccccagg cgctcaggcc
     3241 gcggcgaact gaagagcatc cgggcatcgc gcagtcagtc agggtccttc gcgatgacga
     3301 gcacgcgatt cgacattcct ccggctggag tcgtccatac cgctctgcgc cgcaccaccg
     3361 agacgctggc gagggaactg gctgagccga ccgccacggc acccgactgg tcggaatttg
     3421 aatggctgat tgcgcgcgcc accgctgcaa tgcacggcgt ctcctcgctg ctttcgggtg
     3481 cgctacggtg gcagggacct cccggatggg cgtcgtttct gcaaaaccag cggcaacaca
     3541 cgctcggccg acacgctcgg ctgctggagc ttctgcaacg gatcgaccta catgcccgac
     3601 gcgacggaat tgctctcgtg gcgctcaagg gtgtggaact gcatgcgttg ggcttgtacg
     3661 cgccgggcga gcggcccatg ggcgacatcg acctgctggt gcgggccgag gacggcccaa
     3721 gagcggcccg actgttggtc tcgatgggct tcagcgagag catgcccacc tggaagcatc
     3781 gcgttttcct gccagagcac ccatctcagc cggccgccct gggcgagcat gtcgacaact
     3841 atctcaaagt cgagctgcac gagagggtgc gcgaggcgct cccgctgctt ccgcaggaag
     3901 tgacgcagtg ggtgttcccg cctcggcccg aggctgggtt gaatcgctat ccctcgaagg
     3961 cctcgctgat gacgcatctg ctgctgcacg cggcaggcgc catggcctcc cgctcgctgc
     4021 gcctgctgca tctgcacgat ctcgccgtgc tgtcagagcg aatggccccg caggactgga
     4081 aagagctctg cgcgcaaagc gcccgggatg gcggtcactg gtgggccgcg ccgcccctct
     4141 ggctcaccgc gcgctattac ggcaacgcca ttccagggcg ggtcctcgat gagctcggcg
     4201 cggcgtgtcc gcggctgctg atgcgctttg cccgccggcg cgcgctctcg gatgtctcgt
     4261 tctcgcatct gtggatcagt gctttccccg gtatcgagtg gtcgcaatcc gccgccgatc
     4321 tggcgcgcta tgtcatgagc cgcatgcgcc caagcccgga gatgctcggg ctgcgccggg
     4381 agctggtccg aacgcacctg ggctcaggcg cgagccagtg gaaccggctc tcgcaggcgc
     4441 ggcgcctgct tcggtggctg acctcgcgac cggcgcggag cgagacgatg cacccggtgc
     4501 tgatggccct cgcgcaagca cgccagatat gagcgccgca gatataaccg cgccagcggc
     4561 gagcccgcca ctcgtaagct cgccagcggc gagcacgcca gcagtcccca ccgggctgct
     4621 cggggacacg ccagcgcgcg actacacgcg caagctccaa ctcttcaaca aattcgccga
     4681 gccggagctg cgccgggcga tcgacacgct tgggctgacg cccggaatgc gcgtgctcga
     4741 tgccggttgc ggcagcggcg aggccttgac gtggctcgcg cagcaggtgg gtccgagcgg
     4801 cgaggtcctc ggaatcgatc tcgcgattgc acacatcaac gccgcccgca ggttcgtggc
     4861 tcccaggatc tcggtattgc agggcgatat tctcagcatc ccgctcgcac ccgcgagctt
     4921 cgatgcgatc tggtgtgtca ataccatcaa ccatcttcgg gacgcgatcg ggggcgtacg
     4981 caggctggca tccctgctgc ggcccggggg ccacctggcg ctcggtcaga gctcgctgct
     5041 cgcggacatg tactttgcct gggacgcgcg gctcgagcgc ctgacgaacg aggctgtgcg
     5101 gcagtactat cggacgcggt atggggttgg ggagggtgag cttgcgccgg tgaggtcact
     5161 cgtcgggctg ctgcgcagcg cgccgctgca ggatgtcacc tccaggacct tcgtgatcga
     5221 aaggatcacc ccgctgaagc cggccgatga ggcatatctg cacgaggcaa tttttctgga
     5281 cacgtggggc gagcggttgc ggccttacct tgcggcgcag gatttctcgg ctctgtcgca
     5341 gctctgcgat tcgggggctt cgcactatgc cctgcaccgt cccgacttcc atttcctgca
     5401 gaccttcacc cttgctgtgg gacgcgtctc atcgtgagcg actcagcgct gtcggagcac
     5461 gatcgagccg cggggacttc gccccggctg cgggatacgc gggctgcctt cgatagcgtt
     5521 gctgccgact atgacgggcc tcgcggcaac aacgagctga tccaggatat gcgccgcgag
     5581 atgtggcgct ggctcgatgc gacgtttgcc ccaggcagcc gcctccttga tctcggctgc
     5641 ggcacggggc tcgatgcgat ccgcatggcg cggctcggcc atcgggtgac cgctctcgac
     5701 tggtcggcgc agatggtccg gcgcaccgcc gagcgggctg cccgcgagaa tttgagcgac
     5761 cgtgtccaga cactcacgat cggggctcac gagttgcagc ggctgccttc ctccagtgag
     5821 ttcgatggcg cctactccaa cctcggcccc ctgaattgtg taccggatct gaccgtggtg
     5881 gcgaccgagt gcgcgaggtt gttgaaaccg ggtggcgccc tggtgttcac ggtgatcggt
     5941 cggatctgcc catgggagat catgcattac gcacgtcgca ggcagtgggc acgactgcgg
     6001 gtccgcttcg cccgcggcgt ggtgcctgtt ggaatgaacc ggcataccat ctggacgcgt
     6061 tactaccaac ctcgggagct gtatcgagcc ttcaggccgc agttctccct gagcctttat
     6121 cgcggcctgt gcgtgtttgc tcccccgccc tacctgacat cggtgcgcga aaagcatccg
     6181 cactggcatg agcggctgtg gcgcctggat cggcgctgcg cggggtggcc gctgctgcgc
     6241 gcgatgggcg atcatttcct gatcgtcctg cgaaggcgct gagctggccg gggcgggact
     6301 gcgcgggctg ccttcacagt gaactccatg ctcaaagcac tggtgcgcaa gacggttgga
     6361 cgccgccgca tccacagcct gcagactctc atacgagact cgtccattta cacgcaagag
     6421 ccggcggtca ccgagcctgg tgcgggtaaa gtagtcgtgc tggcaccgca catggatgat
     6481 gagaccttgg gttgtggcgg taccatcgcc cggcatgcgc aggcgggcgc cgaagtgacg
     6541 gtgatctttc tgaccgatgg tcgttacggc gggcgctcct gcccggaagc gacgcgaccc
     6601 gagcgcgagc gtctgcagcg agagctgatc gagacgagaa agcaggaggc ccagcttgcc
     6661 ggacagatac tcggcgtgcg ctcgctgctg tttctcgacg cggaggacac gcaactgcgc
     6721 cgcgaccagc acgtcggggc gcggctgcgt cagatcctgc aacaggaaaa gccggattgt
     6781 gtgtacctgc ccttcttcct cgaacgccac gtcgatcacc gggccgccaa tgaggtactg
     6841 ctcgctgcgg tgtccggtac cgcgctcgag ttccagtgcc gcggttacga gatctccact
     6901 ccgctgtttc ccaattgtct cgttcgcatc gacaccacgt tggagctcaa gcgtcaagcg
     6961 ctctcgtgtt atcacagcca gctcacggat ttcgactacg tccatgcggc aatcggattg
     7021 aacgcgcacc gctccatgtc tctgggcggt cactgcggca gatttgccga ggccttccat
     7081 gctctgccgc ttcgcgaata ccggcagttc tatcacgcgg tgcccgtctc gctccgatga
     7141 cccgcctcga gccacgaagc agcacgtccg ggcccgtgga gcgcgatcat gtcacgggcg
     7201 ccgagtacgt caggcagctc tcagtgcggg agtcggaccg gcgcacccgt gcggcgtttc
     7261 agcatctggc gctcgggctg gtgccgccgg gtggccgggt ctttgatttc ggcgccggca
     7321 ccggcaccga cgcgcgtttc tatgcagagc atgggttgag cgtggccgct tacgacgtcg
     7381 atcctcggat gcaggcgttc ttcgccgagt actgccagga cttcatctcc agcgggcaag
     7441 tcagccttca aggcggcggc tatcgacagt tcctggccga atctgcagct cccaccggca
     7501 ctgacggcgc ccacctggtg accgcgaact ttgcaccgct caatctgatc gcggatctgc
     7561 gcgagctgtt cgagcgattt cacgcgctca cccggcccgg cggccgggtc ctggcgagca
     7621 tgctcagccc ctatttcatt ggtgacctgc gctatggatg gtggtggagg aatcttggcc
     7681 gcctgtggcg tgagggcagc tacgcggtgc aaggcactca gggtcctgtc atccgccgcc
     7741 ggctgtcaca gctcgccacg caaggccttc cctacttcac cttgcagcga gtgttcccgg
     7801 ggttcgcccc caccggttcg gctcacgccg ccggattgag tccattggac tcgggggttg
     7861 acgtacacga tggaccagga tcggcgtggc gctgtgccgt cggctgcctg ttcatgttcc
     7921 tgctctacga gaaacgaagc cccgcggtgt agtgcatcaa acaccgagcg tgaatcgttc
     7981 gagagctgtt gtgcattcat cgtaagccgc cgcagcccgt tccagctccg agctggccag
     8041 atcgatgcgc gtggcagacc tctcgcggat gattctgcct gcgtggatat agggcccagg
     8101 gtcgccgtgg cgcccggtga acgggtacgt ggcgtatgtc gggctgaaac cccggcgaag
     8161 cccgagaaac acgcggagcg cctcgagggt ttcctgcggc gcgtccgtca gggactcata
     8221 gcggatcagc gccgcgttgc cctgcgccga cagactttgc cccagtctcg tcagggattt
     8281 gacccgaccc acgtagtact cgagcgctcg agaggtcgac caaggctgct ggtaatgagc
     8341 gcggtacagc tcgacgatgc ttctcaaact gggctcaggg cgccgcaaaa gaaacaggac
     8401 cttgacacgc cgatgccgca gcaaactcgc gtgaggcgtg agctcatcat gattgacctg
     8461 atcggcgacg tagcgcagca ctcggagcgg gccgccgtgg cgtatccgcg ccgcaagcgc
     8521 cagccgagcg aaatcagcgc tcgaatcgta aacgacaccg cgctcgccga gcgcggcgat
     8581 ttcaggattg gtcatcagca ggtgcagcag cagggtcgag ccgctgcgca tatggcccag
     8641 gagcagcagc actgacgggc gcgccaaccc aactgccttc aggctggctt cggcgctgac
     8701 cagcgtgccc aggatggcgc gcttccccgc tttgcgcagg ccattcacgg gcgtcgaggc
     8761 tggatgtccg gcccgaaaag ggtcggatcg atgaatgacg cccgttcgcg cgcgttgacc
     8821 ccatttaggt gattggagtc cagatacagt gggaagccgc ccgagtctcc cgccggacag
     8881 agcatccccg cgcacaggct cgagcgagga tcgagcactc gcgccccggt ctgcgccgcg
     8941 atctcgcgca atcgatccat caagggtgca acgaacgcct cgtacgctcg cgcatccacc
     9001 tgcggggtta cgtcacgcgg cagatccctg ggcaaatgca gcgccaggcg cagcccgggt
     9061 ggaaagcggg gatcgaactg tggcgatgtg ggattggaga gcacgatgaa tacgcgccga
     9121 ccactcgaca ccaggctcga gatcgccttg cggaatctct cgagagcggt ccgggcctcg
     9181 ggcgagtcca aaggcagcca gacgcgagcg ctcgcgggca gaccgtagac gtggggcacc
     9241 gggccctcag gggagtactg gccgagaaag taatgctccc agaaggcgcc aaatacgacc
     9301 gtgtcgacgt cggcccgacc agcctgcttg atggcgaact cgaagaaccc gccgcatttg
     9361 ggatccggcc acgaacgggg gattcccggc aatggaggac atcccggata ccccgcaaga
     9421 actgccgacc gggcactgtc gggataggtg tcgatcagaa gttgcaggcg tggcctgtac
     9481 tgctggatgt gcgagtcgcc gataaataga gtcttgtgct cacgctgact cgccacggtg
     9541 agcatgccga ctccagtttg tggatccttg gtctcgccgg agtaatgcca gtcgttcacg
     9601 gcctgattcc atctcgcgac cagtgatccg gacaggcgcc ccgggatcca tccccgatcg
     9661 aaagccatgc cagccagcgt cagaagtacg aggcccgcga gcagcggtgc aacggccttg
     9721 cggccgagcc gcccgtagcg tatcggagac tcgatcagac gataggtccc gtaggcggcc
     9781 aggccggcga tgcccatctc gatcgaggcg atgccggcac tcgggtgatg gccgagaacg
     9841 acgagcgtga atgccagcgt gggccagtgc cacagataaa gggggtagct gatcttgccg
     9901 agcaggacca tggggcggct cgcaagaaac ccgccgctca cggtgtgcgg tcccgtcgcc
     9961 agcagcagcg cagcaccagc ggttggaagg aggctccagc tcccgggtat tgccctgtcg
    10021 gtggtccagc acactcctcc cacgacgatc aacatcagcc ccagcaccga gatcgcctgc
    10081 aaactgagcc gggtgggagc ctgcttcgcg gcatcgcgtc cgatcactgc gcgaggtgtc
    10141 aggtcctgcc aggcggccag gatcccaccg gccgccagct cccaagcccg cgtcgccgga
    10201 gtgtagaagt ggggcgtgcc atgccaggcg ctccagatgc tgaaggccag tgacagcgcg
    10261 aacacgcctc ccatgaagtg catcgtgatg ccgcgtttaa ccgccagtgc gagcagcagc
    10321 ggccacacga tatagaactg ctcctcgacg gccagcgacc acagatgcat gagcggattc
    10381 aactccgcgg acggctcgaa atagccgccg cccgttcttg cgaagtacag gttggcgagg
    10441 aatgggacgc accatttgat ggaatggccc aaccatagca gctcgctggg taacatcagg
    10501 taccagccga cgaggcagca tgcggccagg accagcagca gggccggaac gatgcgtcgc
    10561 acgcgccgcc ggtagaagtc gctcaagctg aatgtcccgg cccgcaatgc tctcaagatg
    10621 acctgactga tcacatagcc ggaaatgacg aagaagacat cgacgccgat gaagcctccg
    10681 gttcgaaaca ggggaaaggc gtgataggcg acgacgagcc cgaccgcgag cccacgcaag
    10741 ccatcgatgt ctgcccggta ctggatcgcc gcgtgcgcgt gagagcggcc ccgcacagcg
    10801 cccgctgact gcggtgtgat ggcttgcgcc tgcgatactg cttgcggcag cagtgccagt
    10861 ggcaagttac gagcagacac ccgatcccct gacatttcga tgtgcgttac ccggcgccgc
    10921 gacactggtg tacagggctt ggaaggctcg agcggtgaag tcggcatcct cgcgcacggc
    10981 tcgccgaagc gcctcccgcg caacccgcag ccgcagactc tcatccgcga gcatctcggt
    11041 gatcgcgcct gccagggcgg accaatctcc gacgggcacg gcacctgccg catcaggcgc
    11101 ccattcggcg atatgaccta cggcggtccc gatcgtaggc acaccctggc ctgcggcttc
    11161 gagcaccgca agagggcccg cctcgtgacg cgaggacatg accagcaggt gtgcgtcctg
    11221 caaaagcggg agcagtcgtc gctgcggcag aaagccgtgg aatcgcacac tgcccgccag
    11281 gcccgattgg tgagccatgg cctgaatttc accctgcagt gtgtcctcgc ccacgatgtc
    11341 catctcgaat ggcacgccgg agtgctgcag tgaagccagc gcatgcagca gggtcgtctg
    11401 gtccttgacg cgattcaggc tcgcaacgtg cagcagcctg gcgggcttgg ccgggtcacg
    11461 cctcacaggc gcacgtggtg gccagagctt cacatcgacc cccagcggca cacggcgcgc
    11521 gctgattccg agctgcgcca gtgccgccac catgggatcg ctggctgcgc tcacgaccgt
    11581 cgcggcccgc agtacgagcg cctccctcat gcgccccttc cagccgaggc gacctccata
    11641 gccgatctca ggaagggcca ccagctctcc gccggcgaca tgaatgaggc tcggcaggcg
    11701 cagaatcctc gccgccgcca ccgcgaccag gcccggcgca cccgaccata tcgagtgcac
    11761 cagatgaaat ggcgaggagc gatgttcggc gcagatcgcc tgaatcgccc gcacgcgcga
    11821 atgcgcggcg cctacgttgt gaatgcgcgc gccgagcagt tcccagcagg cgggctcagg
    11881 ctcctgcgcg agagcaaaga cgtgaatctc atcccgaacc gacagccggg cgatgagcgc
    11941 caggagcgct gggatgaccc ggcactctcc ggagcgatcc acaccgccgg ggaccactag
    12001 agctatcttc accgcgcggc tcgcttgctc acgacgtcgg gcgatgactg cagcactcgc
    12061 atcagcgcct caactgcctc atgcggatgt tgcccgcggc gcagctcaaa tgccggcaag
    12121 cgacacgcct gccggacaaa gcgagcccag cccggctgat gcgccgcata cttctgagtt
    12181 gcggcaagcc tttgcatcag ccgcccctgc gccagcggcg caagaagggc gcgcgcacca
    12241 gcgcgcctgg ccgagatgaa agccacggcg cgaatgcgca gcggctgcga ggcgagccga
    12301 tagcgtggtc ggcgcaggtc aatttcgagc ttttccacac cgctgcggcg gcggatgatc
    12361 gacgagccac caaggagcga tgcgcgcgtg cgggcatcaa gaaacttcag agagtcaggc
    12421 cgcgcgtgca gaaaatttgc gattccggtg gcgagcattc catccggatc caccagcaca
    12481 ctgtcctctg ccaggaactg caggccctcc gccagacagt gcagcgagac tgtggatttc
    12541 ccggacccgc tcggacccaa cagcaggata ccctggccgt cgtatccgac acaggcagca
    12601 tgcagaggga ccaggccctg tttgcgagcg gccaacacgt acaccgcaaa ttcgagcatc
    12661 tcgtagcgca cgtggtacgg gaaggcgagc atctctcgtg gcacgaccac cagcgccgag
    12721 ctctgccccg gtgcgattgc cacgaagctc gcggtttcca tggccccgca aagcagtccg
    12781 gatgcggaaa ccgctcgtat ccgcggcggc tcgttctgcg tatcgatcct gtggtgcgga
    12841 ccggtcaggg cgagtcgcac cgaaaagcgc gggcctgcgt tgcgtaacgt atgcgcaggg
    12901 agccctcgat acgcctctcg cacgatccgc agaagctgag ggctgtcggt ctcgaaacga
    12961 aaccttccgc cgagcagctg gaattgctcg cgccgccgtg ggcggtgccg ctctgcgaag
    13021 gggtctgcgg aaggttcagc cagccctggg tgctcgttgc gcgccacgtg agaagccctc
    13081 agcgaagacc cgccatctgc ctgaacgctt ccgagttccg aagcaactcc tcgtaggtcc
    13141 cgctggcgat gaccctgccg tgctccagtt ggatgatcag atcacaggcg cgcacggtgc
    13201 tcatgcgatg tgcgatgagg acgctcgtgt agtggcctcg cagctgacgc agggtcatca
    13261 tcagctcctt ttccgtcaac ccgtcgaggg cgttggtggc ctcatccagc aacagcacgg
    13321 aggcctgccg atacagtgct cgggcgatgc cgatccgctg cctctggccg ccgctcagcc
    13381 tgacacctcg ctcgcccact ttgtgcagca gtccctcagg cagggccccg agcagctcct
    13441 cgagttgtgc ctgacgcgcc gcctcgcgta cgcgtgccgg gtcgatgtcg gccagctcgg
    13501 tccccagagc gatgttttcc gcaatcgtgg catccagcat gaaggtgctt tgcggaacgt
    13561 aagcgacctg agcctgccag ccagcacgct gttcgtcctc gagcggcgcg ccgtcgatta
    13621 agatgcggcc gatgctgggg accaacagtc cggcaatgag gtcggcgagg gtgctcttgc
    13681 ccgaaccgtt cgcgccgacg attccgaccg accgctgcgc cggaatgcac agcgacacgc
    13741 cgcgcaaagc ccacgaatcc gcccccgtat atcggaatga cacctcttcc agccggatct
    13801 cgtgccgcgg gcgtctctgc aagtccagca gtgagggtcg cggtacggcc gtagcggacg
    13861 cttgccgcag ggtttgtgca tgccgcaagt cgggggcaat cagcgcgagc gccgggcgat
    13921 ccgcccggat cttcacgatc aaggcgaata tctgctgcaa cgtcggcagc aagcggtacg
    13981 ccgcaaatgc caggaacgtc agttgcccga gccagggtcc gatcccatcg ctgcgcccga
    14041 gcagcaccag ggcgatccca accaagcccg ccgctgcgac acattccatg agatagcgag
    14101 gactctgcgc gatcagctgg ttgtgagcgg ctgccgcggc gagctctttg ctgaggcgct
    14161 cgaagcggcc ggaaaagtag ctgcgcgctc gccgcagggt gagctccttg atcgcaccga
    14221 agccctcgtt gaccacccgc gcctggtcgg tgctcaggtg cgacagcgcc tgtccacagc
    14281 gcagcacgcg gttgcggaca ctcagataga tcaggccgta cccggcggcg agtacggcga
    14341 tggtcgccag cgtgactgtc ggattcaaca gtacgcacgc gatgatgatg aatccagcgg
    14401 tgacgaggct cgccaccagc gtgagcgtgt tcagcagtat cccgtgagtc actcgaacgc
    14461 tctcgtagac gatgttcttg acgagcagcg cgctgttggt cgccgcgtga aacgagtacg
    14521 gtcgcgagag gtagtcgttg aacaacaccg tttgcagctg attaccgatc cacatcgcca
    14581 ggtgcgccat gatcaaggta cccaccgcat tgatcagatt ggcaaccagg acgacggcaa
    14641 tgaagccgct gccgagcagc acgatgaagt cccgcttgct cgacaggccc aagtgcaggt
    14701 agagccagtg gatcacggca ttgtgatcga tcagttgggg ctcgccgagc accgcaaaga
    14761 agggagcgat cgccgcaatc cccgtgaccg tggaaaaggc catcgccagc actacgagct
    14821 gcgctgcgag catccgccgc cgttgggcgg gggtcaggat gctccagagc tccgacagca
    14881 ggctcatgag aggctcgaaa gagcgcgcag tctggctcgg caatggcgcg cgagcgtccg
    14941 cgcggcctgt ggagcaaatg caacggccgt cgcgcgcagc gcgccgagcc agcatcctcc
    15001 atatgccaag gagtgcggca ggctcgtcag cagggtcgtg agagcatcga gccgttggcc
    15061 tcccgccgcc tggcttcggg cgaggccagc cgacatctgc gcgcgcagtt tgcgcagcag
    15121 cggctcgagc tggccgccgc cgctggtgcg taacgccttc tcaaagacgc gcctgcgatc
    15181 gcgccaggcg atgatgtcgc tcccggagtg acttgcatgg cggcgcacta gcgtaagggg
    15241 cgcatcgatt ccgtcgatct cgctgtgagc ggccaggcgc agccaaagct cgtcgtcata
    15301 acacatgagc agcgactcat cgaacgggcc cagccgctcc agcaactccc ttgatacgac
    15361 cacactgggc tgagcg
//
LOCUS       DASRHN010000435         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_1150227, whole genome shotgun sequence.
ACCESSION   DASRHN010000435 DASRHN010000000
VERSION     DASRHN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150227"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="lpxC"
                     /locus_tag="VFB37_17670"
     CDS             <1..618
                     /gene="lpxC"
                     /locus_tag="VFB37_17670"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZO24326.1"
                     /translation="IMDGSAGPFVFLLQSAGIEEQREPKKFIRVKKRVRVDDGDKWAQ
                     FDPFAGFKVNFEIEFNHPIFKRRAQKASMDFSTTSFLKEVSRARTFGFMRDLETMRAR
                     NLALGGNLDNAIVLDDFRVLNEDGLRYEDEFVKHKILDAIGDLYLLGHSLIGEFSGHK
                     SGHALNNALLRMLLADTSTWEEVSFNSYADAPISYIEAPVLPGSG"
     gene            complement(624..992)
                     /locus_tag="VFB37_17675"
     CDS             complement(624..992)
                     /locus_tag="VFB37_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24327.1"
                     /translation="MPNKRHRDKRGGTRFPATDRSKSFRRAHSVKDLLGHLTPTLTRV
                     SDQLSTQTFWQTWLAGQLPAALCQRVSAVEESEGTLVIYSESAAWSARLRFAVQDLTP
                     AARAARRGILQISVRVLPKA"
     regulatory      1001..1084
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1208..2128
                     /locus_tag="VFB37_17680"
     CDS             1208..2128
                     /locus_tag="VFB37_17680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZO24328.1"
                     /translation="MNIIFFSKREGLARQLNLAHPLTLGVIGALVLGILGTAFALGVQ
                     LGQGSNGRMALGESARWTAILADQKRQIAELKESMAERVDAMAMRMGAVNAHVIRLDA
                     LGKRLTEMADIDSHEFDFDTEPPSGGPEADGGGVSAQIPDLSAKLKDLEQTVELRDSQ
                     LTALENVILARELKEETHPAGRPVSGGFISSYFGERMDPFSGEEAYHKGVDFAGTAGA
                     SVTAVAAGVVTWAGERAGYGKLVEINHGDGYVTRYGHNEKTLVAVGQTVKRGDPIALL
                     GSTGRSTGPHVHFEVIRNGRQVDPLSFVGR"
     gene            2333..>2662
                     /locus_tag="VFB37_17685"
     CDS             2333..>2662
                     /locus_tag="VFB37_17685"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24329.1"
                     /translation="MLHFLTRIFGSRNERLVRSYGRYVREANEFEPTIQALSDEALRA
                     RTDEFRARLKQGTPLNDLLPEAFAVVREAARRTLKMRHFDVQLVGGISLHEGKISEMR
                     TGEGKTLV"
BASE COUNT          499 a          825 c          870 g          468 t
ORIGIN      
        1 atcatggacg gcagcgcggg ccccttcgtc ttcctgctcc agtcggccgg catcgaggag
       61 cagcgggagc cgaagaagtt catccgcgtc aagaagcggg tgcgcgtcga cgacggggac
      121 aaatgggcgc agttcgatcc gttcgccggc ttcaaggtga atttcgagat cgagttcaat
      181 cacccgatct tcaagcgccg cgcgcagaag gcctcgatgg acttctcgac cacctccttc
      241 ctgaaggagg tcagccgcgc ccgcacgttc ggtttcatgc gcgacctcga gacgatgcgc
      301 gcgcgcaatc tcgcgctcgg cggcaatctc gacaatgcga tcgtgctcga tgactttcgg
      361 gtgttgaacg aggacggact gcgctacgag gacgagttcg tcaagcacaa gatcctcgat
      421 gcgatcggcg atctgtacct gctgggccac agcctcatcg gcgagttcag cggccacaag
      481 tccggccatg cgctcaacaa tgcgctgctg cgaatgttgc tggccgacac atcgacgtgg
      541 gaagaagtct ctttcaactc ctacgccgat gcaccgatct cctacatcga agcgcctgtt
      601 ctgcccggct caggctgagt ttctcaggcc tttggcagca cccgcacgct gatctggaga
      661 atgccgcggc gtgccgcgcg tgcggcaggc gtgagatcct gcaccgcaaa acgcagccgt
      721 gcagaccaag ccgccgattc cgagtagatc accagcgttc cctcggattc ctccactgcg
      781 gagacgcgct ggcataatgc ggccggcaac tgtcccgcca gccacgtttg ccagaatgtc
      841 tgtgtgctga gctgatccga gacccgtgtc aaagtaggag tcaggtgccc cagcaggtct
      901 ttgacagaat gggcgcggcg gaacgatttt gagcggtccg tcgccggaaa gcgggtaccg
      961 ccgcgcttgt ctcggtgtcg cttattgggc atattgaaat cgctgatttg ggcaaacccg
     1021 ttgaaaggcg gggacgcaaa gccaccggtc taaagcgagg agccatgaca gcggggttgc
     1081 cagcacagtg cacgctattc gaattaagtg aacgagaaca gggcgtgaca cgcccgggct
     1141 acggccgtgc ggtgatgcag ggcaagcccc tcgagacggg gtatcgcacg gatccggagc
     1201 gcgcggcatg aatattatct ttttttcgaa gcgagaaggg ctggcgcgcc agctcaacct
     1261 cgctcacccg ctgaccctcg gggtcatcgg cgccctcgtg ctgggtatcc tgggaacggc
     1321 tttcgcgctc ggggtgcagc tcgggcaggg cagcaacggt cggatggcgc tcggcgagtc
     1381 ggctcgctgg accgccattc tcgccgatca gaagcgccag atcgccgagc tcaaggagag
     1441 catggccgag cgggtcgatg ccatggcgat gcgcatgggc gcggtgaatg cccacgtaat
     1501 ccgcctcgat gcactcggca agcggctcac ggaaatggcc gacatcgaca gccacgaatt
     1561 cgacttcgac accgagccgc cgagcggcgg ccctgaggcg gacgggggcg gcgtgagtgc
     1621 ccagatcccg gatctctcgg ccaagctgaa agacctcgag cagaccgtcg agctgcgtga
     1681 ctcgcagctg accgcgctcg agaacgtcat tctcgcgcgc gagctgaaag aggagacgca
     1741 tccagccggg cggccggtct ccggaggctt catttcctcg tatttcggtg agcggatgga
     1801 ccccttctcg ggcgaggagg cctaccataa aggtgtggac ttcgccggga ctgcgggcgc
     1861 cagtgtcacc gcggtggctg caggtgtggt gacctgggca ggcgagcgcg ccggctacgg
     1921 caagctggtc gagatcaatc atggtgatgg ctatgtgacg cgctacggcc acaacgagaa
     1981 gacactcgtg gccgtaggcc agaccgtgaa gcgaggcgat ccgatcgcgc tgctgggctc
     2041 cacgggccgc tccaccggac cgcacgtcca tttcgaagtc atccgcaacg gccgtcaggt
     2101 cgatccgctg agcttcgtcg gccgctgagc gcggcgaagg cccttccgag gctgcccagc
     2161 agggccgcct gctctccgct ctcttgcctt gaaaatcctc ccgggcgaac caattgggcg
     2221 ccaggagcgt ctattgcgta gaatacgcgg ctttcaaaag ccccggtggg atgcgccagc
     2281 cgcgagataa cggcttgtcg cgccgggcat ttttcctatt caggacgtca agttgctgca
     2341 tttcctcacg cgcattttcg gcagccgcaa cgagcggctg gtccgaagct acggccgtta
     2401 cgtccgggag gccaacgagt tcgagccgac gatccaggct ctgagcgacg aggcgctgcg
     2461 ggcccgaacg gatgagttcc gggcccggct caagcagggt acgccgctca atgacctgct
     2521 ccccgaagcc ttcgccgtcg tgcgtgaggc ggcccgccgc acgctgaaga tgcgtcactt
     2581 cgacgtgcag ctcgtgggtg gcatctcgct gcacgaaggc aagatctcgg agatgaggac
     2641 cggcgagggt aagaccctcg tg
//
LOCUS       DASRHN010000436         5881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2462752, whole genome shotgun sequence.
ACCESSION   DASRHN010000436 DASRHN010000000
VERSION     DASRHN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462752"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFB37_17690"
     CDS             <1..622
                     /locus_tag="VFB37_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HZO24330.1"
                     /translation="QHHVGGYLKIAPEAIAEGPLSKMMKPGVGAYYRFRELFDRYSRE
                     AGKEQYLIPYFIAAHPGTSDEDMLELALWLKKNGYRADQVQAFLPSPMATATAMYHSG
                     RNPLRRITHDGEQVRVPKGLRIRRLHKAFLRYHDAANWPLLREALRRMGRADLIGSGR
                     HQLVPAFQPRNAGAEPAGRRGGSSGRASRPFRTQHTGLPRNPRGAR"
     gene            619..2070
                     /locus_tag="VFB37_17695"
     CDS             619..2070
                     /locus_tag="VFB37_17695"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HZO24331.1"
                     /translation="MNSRAARRWIGGALALAGPIAYGATGAPATEPQRGAPLATVLEV
                     NGAIGPATSRFVVHGIETAQHEGNRLVVLQIDTPGGLDTSMRDIIRAILASPIPVVGY
                     VAPPGARAASAGTYILYACHIAAMAPATNLGAATPVSIGGESAAPPEPERKPDSTDHD
                     KEASSGKKPSEGAAPKSEPPANQSAEPVPGSAMERKVVNDAVAYIRGLAELRGRNADW
                     AEQAVRGAASLSATAAVQQKVVDLMARDVPDLLNQLDGREIRVGNQLLKLQTRALAVT
                     YVKPDWRIRLLAVITHPTIAYGLLLIGIYGLLFEGYNPGAVFPGVVGAISLLIALFAF
                     QLLSVNIAGLALVAVGVGMIIAEFFFPAFGSLGLGGLVAFVVGSLILFDTDTPGMNIG
                     LPLIAGVATVGGLVIIATAWLAARSLRRPVVTGTQAMVGELAEVFEDFAGRGRVRYGG
                     EIWNARSPAETVRSGQTVRIVRVEGLTLWVEPL"
     gene            2105..2848
                     /locus_tag="VFB37_17700"
     CDS             2105..2848
                     /locus_tag="VFB37_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZO24332.1"
                     /translation="MPYGIVVIVLILLLIFSTFRVLNEYERGVMFTLGRFTGVKGPGV
                     VLVFPILQHMRKVDLRVIVLNVPKQDVITRDNVSVKVNAVVYFRVVDPGKAIIQVANP
                     FEATSQVAQTTLRSVLGQHEMDDLLAQRDKLNADVQRILDQNTEAWGIKVSNVEIKDV
                     DLDETMVRAMAKQAEAERTRRAKIIHAEGESQAAQTLVQAAQSLARQPSAMQLRYLQT
                     LADISHERTQIIVFPLPLDLIKPLLDPRT"
     gene            2848..3252
                     /locus_tag="VFB37_17705"
     CDS             2848..3252
                     /locus_tag="VFB37_17705"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZO24333.1"
                     /translation="MPIEPRALHGRWPLVAAGFLLAAATALGAMGAHALKSQLSPERL
                     AIYDTAVRYHFLHALGLLAIGILLRTLDSDLLRWSAIAIVAGIVLFSGSLYLLTFGLD
                     PGSARIVGLATPLGGLLLIAGWVVFAVAMLRL"
     gene            3271..5364
                     /locus_tag="VFB37_17710"
     CDS             3271..5364
                     /locus_tag="VFB37_17710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZO24334.1"
                     /translation="MYRRFAGSAPLLLALAACTHSPPAPTASTTESTSAAGAQTAEPT
                     PRHAQIGDWGLDLTARDPSVKPGDDFYRYAEGHWLDTTQIPADRTVWNAFSQLADKAQ
                     HDLRSIAESLPADAPEGSTQRKVGDYYRAYLNTDAIERAGLGPAQPALSAIAAARTYE
                     DLARLMGRRDLSLRAPLRLLITIDQKNPDRYIAAISQSGLGLPDRDYYLKNEAVFTDL
                     RAKYSAHIARMLELAGAKTAPTDASSILDIETRIARLHWPAAKRRERDLTYNLRTRAQ
                     LEQLARGIPWRALLASQGLDSRQAFIVRELDAVQGLAKLFRQIPVKRWRAYFQYHYLA
                     EVADVLPKAFDEEYFAFYGHALSGQPQQQERWKRAVDGLDADLGEAVGQLYVQRYFPP
                     AAKEQMLALVENLRAAYAVRIEHLSWMSPATKKVALEKLAAFHPKIGYPDHWRDYSAL
                     EIRAGDAFGNRTRARLFDWQRELKRIDQPTDRGEWGMTPQTVNAYYNPTFNEVVFPAA
                     ILQPPFFDPDADAAVNYGGAGASIGHEMGHGFDDQGSKSDARGVLRTWWQPEDEKAFH
                     TLVERLASQYDAYEPLPGLHVNGHLTLGENIGDLGGLSVALEAYHISLQGRPSRALDG
                     LAGDQRFFLSWAQVWRSLYRHEQLRKLVMSNPHSPSKFRVNGPMRNIDAWYAAFDVQP
                     GEKLYLAPEDRVHIW"
     gene            5371..>5881
                     /locus_tag="VFB37_17715"
     CDS             5371..>5881
                     /locus_tag="VFB37_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO24335.1"
                     /translation="MRSGFPIDEALPALSRALATHCSVVLQAPPGAGKSTVVPLALLE
                     QPWMLGKRIIMLEPRRLAARAVAQRMSWTLREDIGRTVGYRMRMDTRVSSQTRIEVVT
                     EGVLSRLLHADPALEGVGAVLFDEFHERSLQADLGLALVLDARSHLSPELKVLVMSAT
                     LDGAAVARML"
BASE COUNT         1028 a         1904 c         1906 g         1043 t
ORIGIN      
        1 gcagcaccat gtgggcggct atctgaagat cgcacccgag gcgatcgcgg agggcccgct
       61 ttccaagatg atgaagcccg gtgtgggcgc gtattaccgc ttcagagagc tgttcgaccg
      121 ctactcgcgt gaggccggca aagagcagta cctcattccg tatttcatcg ccgcgcatcc
      181 cggcacgagc gacgaggaca tgctggagct ggctctgtgg ctgaagaaaa acggctatcg
      241 tgcggaccag gtgcaggcct ttctgccctc gccgatggca acggccaccg cgatgtatca
      301 ttcgggaagg aatccgctgc ggcggatcac gcatgacggc gagcaggtcc gcgttcccaa
      361 gggcttgcga atacgacgcc tccacaaggc cttccttcgt tatcacgatg ccgccaactg
      421 gccgctgctg cgtgaggctt tgcggcggat gggtcgcgca gatctgatcg gcagcggacg
      481 acaccaactc gtccccgcct tccagccccg caacgccggt gctgaaccgg caggccggcg
      541 aggcggcagc tcgggccggg cgagtcgccc tttccgcacg caacacacgg gactgccgag
      601 aaatccgcgc ggtgctcgat gaacagccgc gcggcacgcc ggtggatcgg tggtgcgctc
      661 gccctcgcag ggccgatcgc ctatggcgca acgggcgcgc cagcgaccga accgcaaagg
      721 ggcgcaccgc tggcaaccgt gctcgaggtc aatggcgcga tcggtcctgc gacttcccga
      781 ttcgtcgtgc acggaatcga gacggctcaa catgagggca accggctcgt cgtactccag
      841 atcgacactc ccggcggcct ggacacctcg atgcgcgaca tcatccgagc gatcctggcg
      901 tcccccatcc ctgtcgtcgg ctacgttgct ccgccgggag cccgcgccgc cagcgcaggg
      961 acttacatcc tgtatgcctg ccacatcgct gcaatggccc cggcgaccaa cctcggcgcc
     1021 gctacgccgg tctccatagg aggcgaatcc gctgcgcctc ccgagcccga acgcaagccc
     1081 gactccaccg atcatgacaa ggaggcgagc tccggaaaaa agccgtccga aggcgcagcg
     1141 cccaagagcg aaccccccgc gaatcagtcc gctgagccgg ttcctggctc ggcgatggag
     1201 cgcaaggtcg tgaatgatgc cgtggcatac atccgcggac tcgcggagct gcgcggccgg
     1261 aatgcagact gggccgagca ggccgtgcgc ggtgctgcca gtctgtccgc cacggccgcc
     1321 gtgcagcaga aggtggtcga cctcatggca cgcgacgtgc ccgatctgct caatcagctc
     1381 gacggacgcg agatccgggt cggcaaccag ctccttaagc tgcagacccg cgctctggcg
     1441 gtaacctacg tcaagccgga ctggcgaatc cggctgctgg ccgtgatcac ccatccgacc
     1501 attgcctacg gactgctgct gatcggcatc tacggtctgc tgttcgaagg gtacaatccg
     1561 ggcgccgtgt tcccgggtgt cgtcggcgcg atctcgcttc tgatcgcgct gttcgcattc
     1621 cagcttctgt cggtgaacat cgcagggctg gcgctcgtgg cggtcggggt gggcatgatc
     1681 atcgcggagt tcttcttccc ggcattcggc tcactcgggc tggggggcct cgtggcgttc
     1741 gtcgtcggct cgctcatcct gttcgacacg gacacgcccg gcatgaacat cggcttgccg
     1801 ctcatcgcgg gtgtcgccac cgtgggcggc ctcgtgatca ttgcgacagc ctggcttgcc
     1861 gcgcgctcgc tgcggcggcc tgtcgtgacc ggcacgcagg ccatggtcgg cgagcttgcc
     1921 gaggtgttcg aggacttcgc cggcaggggg cgggtgcgct acgggggcga gatctggaac
     1981 gcacgcagcc ccgccgagac agtgcgatcg ggacagacgg tccggatcgt gagggtcgag
     2041 ggtttgacgt tgtgggtcga gcctctctga caggagaggt cgggacttcg gagcggagat
     2101 tgccatgccc tacggaatcg tagtcatcgt actcatcctg ctgctgatct tcagcacctt
     2161 ccgggtgctg aacgaatacg agcggggtgt catgttcacg ctcggccgct tcaccggtgt
     2221 gaagggcccg ggtgtcgtgt tggtattccc aatcctccag cacatgcgca aggtggatct
     2281 gcgcgtcatc gtgctgaacg tgccgaagca ggatgtcatc acccgggaca acgtatcggt
     2341 caaggtcaac gcggtggtgt actttcgcgt ggtagacccg ggcaaagcca tcatccaggt
     2401 cgccaatccg ttcgaggcca ccagtcaggt ggcgcagacc acgctccgct cggtcctcgg
     2461 tcagcatgag atggacgatc tgctggcgca gcgcgacaag ctgaatgcag acgtgcagag
     2521 aatactcgat cagaacacgg aggcctgggg catcaaagtg tccaatgtcg agatcaagga
     2581 tgttgatctc gatgaaacca tggtccgcgc catggccaag caggcggaag ccgagcgcac
     2641 gcgtcgcgcg aagatcattc acgcggaggg cgagagtcag gccgcccaga ccctggtgca
     2701 ggcggcgcag tccctggcgc gccagccgag cgcgatgcag ctgcggtatt tgcagacact
     2761 cgcggacatc tcgcacgaga ggacgcagat catcgtattt ccgctgcctc tcgatctcat
     2821 caagcctttg ctggatccaa ggacctgatg ccgatcgagc cgcgagctct gcacgggcgc
     2881 tggccgctcg tcgcggccgg cttcctgctc gccgccgcca ccgccctggg ggcgatgggc
     2941 gcgcacgcgc tgaaatccca gctctcgccc gagagactcg ccatctatga cactgcagtg
     3001 cgctaccact ttctgcatgc gctcggtctt ctggcaatcg gaatcctgct gcgaacgctc
     3061 gactcggacc tgctgcgctg gtcggcaatc gcgatcgtcg ccggcatcgt gctgttttcc
     3121 ggcagtctct atcttctgac cttcgggctc gatccgggat cggcgcgcat cgtcggactg
     3181 gccactccgc tcggcgggct gctcctcatc gcggggtggg ttgtgtttgc cgtggcaatg
     3241 ctgcggctgt aagtcacgga agggccgaac atgtacagac gattcgcggg gtctgctccc
     3301 ctcctgttgg ctctggccgc gtgcacgcat tcgccgcccg cgccgacggc atcgacgacg
     3361 gaaagcacct ctgcagccgg tgctcagacg gctgaaccga cccctcgcca tgcgcaaatc
     3421 ggtgactggg gcctcgatct caccgcgcgc gatccgtcag taaagccggg cgatgacttt
     3481 tatcgctacg cggaaggcca ttggctggac accacgcaga taccggcgga ccgcacggta
     3541 tggaatgcat tctcacagct tgccgacaag gcgcagcacg atctgcgttc gatagccgag
     3601 tccctcccgg cggatgcgcc ggagggcagc acgcaacgca aggtgggaga ttattatcgg
     3661 gcctatctga acactgatgc gatcgagcga gccggtcttg gaccggcgca gcctgctttg
     3721 agcgcgattg ctgcggcgcg cacctacgag gacctggcta ggctcatggg tcggcgcgac
     3781 ctgtccctgc gggcgccgct gcggctgctg atcacgatcg atcagaagaa tccggatcgt
     3841 tatatcgcgg ccatcagcca aagcggactg ggtctgcccg atcgcgacta ttacctcaag
     3901 aacgaggcgg tattcaccga tttgcgcgcg aagtactcgg cccacatcgc gcgcatgctc
     3961 gagctcgccg gcgcaaagac cgcgccaacc gacgccagct ccatcctgga catcgagacg
     4021 cgcatcgccc gactccactg gcccgcggcc aagcgccgcg agcgcgacct gacctataac
     4081 ctgcgcaccc gcgcccagct cgagcagctc gcgcgcggca ttccatggcg ggcgctgctt
     4141 gcgtcgcagg ggctcgactc gcgtcaagcg ttcatcgttc gcgaactgga cgccgtgcag
     4201 ggtcttgcaa aactgttccg gcaaattccg gtcaagcgct ggcgagccta cttccagtac
     4261 cactatctcg ccgaggttgc cgatgtcctg ccaaaggcat tcgatgagga atatttcgcc
     4321 ttctacgggc atgcgctcag cggtcagcct cagcagcagg agcgctggaa gcgtgctgtg
     4381 gacggcctcg acgcggacct cggcgaggcg gttggacagc tctatgtaca gcggtatttc
     4441 ccgcctgccg cgaaggagca gatgctggca ctcgtcgaga atctgcgcgc tgcctacgcc
     4501 gtacgcatcg agcatctgag ctggatgagc ccggctacca agaaagtggc actcgagaag
     4561 ctcgctgcct tccatccgaa gatcggctat ccggatcact ggcgggacta ctcggcgctg
     4621 gagatccgcg ccggcgatgc cttcggcaac cggacgcggg cacgcctctt cgattggcag
     4681 cgcgagctga aaaggatcga tcaacccacg gatcggggcg agtggggcat gacacctcag
     4741 acggtcaatg cttattacaa cccgaccttc aacgaagtcg tcttcccggc cgccattctg
     4801 caaccgccct tcttcgatcc ggacgccgac gccgcggtca actacggggg tgccggcgcc
     4861 tcgatcgggc acgagatggg ccatggtttc gacgatcagg ggtcgaagtc ggacgcgcgc
     4921 ggcgtgctgc gcacgtggtg gcaacccgag gacgaaaagg cctttcacac gttggtcgag
     4981 cggctggcct cccagtacga cgcgtatgag cctttgccag gactgcacgt gaacggtcat
     5041 ctgacgctgg gcgagaacat cggtgacctc ggagggctga gcgtcgcgct cgaggcctac
     5101 cacatttccc tgcagggcag gccgtcccgc gcgctcgatg ggctggccgg cgatcagcgc
     5161 ttcttcctct cgtgggccca ggtctggcgc tcgctgtatc gtcacgagca gctgcgcaag
     5221 ctagtgatga gcaacccgca cagcccgtcg aagttccgtg taaacggacc gatgcgcaac
     5281 atcgacgcct ggtatgcagc attcgacgtg caaccgggcg agaagctgta cctcgcgccc
     5341 gaggatcgtg tgcacatctg gtagggatcg gtgagatccg gattccccat cgatgaagca
     5401 ttgcctgcgc tcagccgcgc gctggcgaca cattgctcgg tcgtgctgca ggcgccccct
     5461 ggcgccggta aaagcaccgt cgtgccgctt gcgctgctcg agcagccctg gatgctcggt
     5521 aaacgcatca tcatgctcga gccgcggcgc ctggctgccc gagcggtcgc gcagaggatg
     5581 tcgtggacgc tgcgcgagga tatcggcagg acggttggtt accggatgag gatggacacg
     5641 cgtgtctcga gccagacgcg catcgaggtg gtcacggagg gcgtgctgag ccgactgctc
     5701 catgcggatc cggcactgga gggagtgggc gcggtcctgt ttgacgaatt tcacgagcgc
     5761 agcctgcaag ccgatcttgg actggccctc gtgctggatg cccgcagcca cctctctccg
     5821 gagctcaagg tactggtgat gtccgccacg ctggatggcg ccgcggtagc gcggatgctc
     5881 g
//
LOCUS       DASRHN010000437         8622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_658935, whole genome shotgun sequence.
ACCESSION   DASRHN010000437 DASRHN010000000
VERSION     DASRHN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658935"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFB37_17720"
     CDS             <1..743
                     /locus_tag="VFB37_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HZO24336.1"
                     /translation="FEIEREIAGSLRPGLDHQVAHARLQWWREECERCVSGRPAHPLT
                     RALLAAFADAPRAGELEGLTGLVDTATWDLAGATFESRRELTAYCGRWAAAIAQPIVR
                     HSTSAEVDWRTVGVALCELEQLIRVDREALAGHIRLPLDELQAAGVESEALAHPPWPP
                     PLAHLLAERHAKLRATLAQAVDGLAPEQQRSLRGLLVWARLCAKLSERAQRSLPAIPA
                     QRRRDSLAATWQAWDTARRAANGRLRLA"
     gene            753..1517
                     /locus_tag="VFB37_17725"
     CDS             753..1517
                     /locus_tag="VFB37_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HZO24337.1"
                     /translation="MSVSFDPREYEPAADELSGRIIAITGASSGIGRAVAMDCAAHGA
                     SVILIGRNAGKLEAVHSQIEARGAPEPTIALLDLEKALAADYDQLASAVVERFGRLDG
                     LLHNAGILGTLSPIEHHDVPTWCRVLHVNVTAAFALTQVLLPALRRSPDASILFTSSS
                     VGRHGRAYWGAYAVSKFATEGLVQVLAAELENTSSVRVNAVNPGKARTMMRRQAYPAE
                     DLNSLPPPETLTGPYIALLGPGSRGVTGMSFDCQRD"
     gene            complement(1483..2325)
                     /locus_tag="VFB37_17730"
     CDS             complement(1483..2325)
                     /locus_tag="VFB37_17730"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HZO24338.1"
                     /translation="MRPRSGPDAGSHRADRPGGPERLQKVLARAGLASRREVESWIRA
                     GRITINGELAALGVRVGPGDQVRLDGRLIRQRGAPETERIFLCHRSPGEPLQPPARRG
                     AGAAREGAPPEGSGTALLERLPKRAGRRFMAISPMPRIDGGLELVTSDGELASRLQRC
                     VKRLTSEFSVRVHGELSTRQLDSIVAGQLDSGVSLAVERCEPAGGEGSNRWYAFAALG
                     ASGKQVRQLFERQGALVSRVLRTQLGPLLLDPHLPRGHFRELTHEEREDLLASPADSR
                     SSSL"
     gene            complement(2338..3075)
                     /gene="scpB"
                     /locus_tag="VFB37_17735"
     CDS             complement(2338..3075)
                     /gene="scpB"
                     /locus_tag="VFB37_17735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZO24339.1"
                     /translation="MNESQVRNVVEAALLAAGKPLHPAELVELFDEGVRPTTAEVREA
                     LETLAEEYGNRGIELKETAAGFRIQVRRELANEISRLWPERPPRYSRALLETLALIAY
                     RQPITRAEIEAVRGVAVNPNIVKTIIERNWVRVVGHRDVPGRPELLGTTRDFLDYFGL
                     RTLDELPPLAELKALDDIKVQFDLPEAVVAQLVSGEPAAEPGPVPTTDLDADLTSSDE
                     DAGDDEELSAAAPDSSELVAGPRQLDD"
     gene            complement(3068..4078)
                     /locus_tag="VFB37_17740"
     CDS             complement(3068..4078)
                     /locus_tag="VFB37_17740"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZO24340.1"
                     /translation="MKLKIVVSNTPAAPTAPQPPAAAAEPSPQQVEMPFAVVNGEPVT
                     QMPRDLYIPPQALEIFLEAFEGPLDLLLYLIRRQNLNILDIPLAEITRQYMQYIELMT
                     ELQLELAGEYLVMAATLAEIKSRMLLPRSSLDPEGIEEDPRAELVRRLQEYERFKRAA
                     EGIDALPRLERDVWTTSAELRDRKVVRLLPQITLQEMLIAFKEVVVRAEMFAHHHIQR
                     ERLSVRERMGDILSTLEHSSFVEFARLFRAEEGRMGVTITFVAILELMREGLIDIVQA
                     EPFAPLHVRAAAPNRVLRIVADNEDRVAPEAVEASAGELVAEQELPVAEPPVSPAEDT
                     HE"
     gene            complement(4103..5317)
                     /locus_tag="VFB37_17745"
     CDS             complement(4103..5317)
                     /locus_tag="VFB37_17745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZO24341.1"
                     /translation="MSATAPARRVLSGMRPTGRLHLGNYHGALRNWVNLQYEYECFFF
                     VADWHMLTTGYEDTSQLQEQIREVLIDWLAAGLNPGVSTLFVQSHVPEHAELHLLLSM
                     VTPLGWLERVPSYKDQQEQLKEKDLATYGFLGYPLLQSADILMYRAAFVPVGEDQVAH
                     VEMTREIARRFNHLYGREPDFEAKAERAVKSLGGRNTTAYKQLRRKFQESGDVQALER
                     ARALVQSNNRITVADRERLLGFLEGTGVTILPEPQVLLTATPKVPGLDGRKMSKYYGN
                     TIGLREDPEVVTQKLRAMKTDPARVRRTDPGDPEKCPVWDLHRIYSDESTRKWVNEGC
                     RTAGIGCLDCKQPVIDRIVEEIGNMRRRAAEFEENPELVRGIVAEGAEKARDAARETL
                     DEVRRAMHVRAD"
     gene            complement(5379..6032)
                     /locus_tag="VFB37_17750"
     CDS             complement(5379..6032)
                     /locus_tag="VFB37_17750"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZO24342.1"
                     /translation="MPSEYFGLHPVNPQWRLVRRAAEIVSRGGVIAYPTDSCYALGCH
                     INDKNALERLRTIRRADRHHHFTLVCANLTDVGRYARLDTWQFRLLKVCTPGPYTFLL
                     HATRETPRRLQHERRRTIGVRIPAHPVPHMLLTELGEPLMSSTLMLPGDALPLTHGRD
                     IQERLEHEIDAVLDGGDCGIEPTTVVDLSSSPPVIVRAGKGSLAPLLGPGAARAPAL"
     gene            6108..6677
                     /locus_tag="VFB37_17755"
     CDS             6108..6677
                     /locus_tag="VFB37_17755"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HZO24343.1"
                     /translation="MQALLELAPLIAFFVAYRVWGLYVATAVLMGGMLVLVITDYLRL
                     RRIPAMHGLSTVLVLVFGTATLVLHNLRFIQWKPTVFFWLASLAFLASFWLGKQTLTQ
                     RLLGAALAGEDSKVSQGTWKRLNTLWVLFYALLGALNLLVAFNFSEHAWVDFKVIGLP
                     VVNFLFAGAQIAWLMRRAPSGSGEPSPQA"
     gene            6708..6980
                     /locus_tag="VFB37_17760"
     CDS             6708..6980
                     /locus_tag="VFB37_17760"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZO24344.1"
                     /translation="MASIDIEQLRTALERGLNPLSLEIIDDSRHHAGHAGAKEGGHFR
                     IRVIASAFAGRSQLERHRLVYAAVAPLMGHGVHALSIDAHAPEENT"
     gene            7010..7855
                     /locus_tag="VFB37_17765"
     CDS             7010..7855
                     /locus_tag="VFB37_17765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO24345.1"
                     /translation="MSYYRLLLVATALALVACQPKGASTAAAPAAADKSPEVASVNGT
                     VITRNFYDEYIKAVTGKSSGDLTPEQRNDALDRLIRMELVSQQAVKDGVDRTPDTAAM
                     LELTRLNVLQQAVSEQYLKDRKPTEQELRAEYETQLAGLSKQEYHAKHILVATEPFAQ
                     KVVQQLEKGAKFEDVAKRESMDASKDNGGDLGWFTPDRMVKPFADAVVALKPGEYTHT
                     PVHTDYGWHVIELVEVRDLQPPAFDNVRQRLEQVVQAKKLKAYTDELLKGAKVDKQLD
                     QKKSS"
     gene            8201..>8622
                     /locus_tag="VFB37_17770"
     CDS             8201..>8622
                     /locus_tag="VFB37_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO24346.1"
                     /translation="MNAKGKYSIWAAGALAALLAASTPAFAQNHGGGGGHGGGGGGHF
                     GGGGHASMGHFSGGGARMGGAHYAPRAGAYAGHGYAGAHVGSGFHGTTVSSAAWHGGS
                     RAGWAGGHAGWGGRAGWGRPGYGHGFHRGFWGGGYWHGG"
BASE COUNT         1429 a         2844 c         2854 g         1495 t
ORIGIN      
        1 ttttcgagat cgagcgggag atcgcaggca gcctgcgacc ggggctggat catcaggtcg
       61 cgcatgcccg actgcagtgg tggcgcgaag aatgcgagcg ctgtgtgtca ggcaggcccg
      121 cgcaccccct gacgcgcgcg ctgttggcag ctttcgcgga tgcccctcgc gcaggtgagc
      181 tcgagggcct gacggggctg gtcgacacgg cgacgtggga tcttgcgggt gccaccttcg
      241 agtcgcgccg cgagctcacg gcgtattgcg ggcgctgggc ggcagccatt gcgcagccca
      301 tcgtgcggca cagtacttcg gccgaggttg actggcggac ggtgggtgtt gcgctgtgcg
      361 agcttgagca gctcattcgg gtggatcgcg aggcactcgc ggggcacatc cgcctgccgc
      421 tcgatgagct gcaggccgca ggagtcgagt ccgaagccct cgcgcatccg ccctggccac
      481 caccattggc gcacctgctg gccgagcgtc atgccaagct gcgcgccacc ctcgcacagg
      541 ccgtggatgg cctggccccg gagcagcagc ggtcgctgcg cggcctgctc gtctgggcga
      601 ggttatgtgc gaagctctcc gaaagagctc agcgttcttt gccggcaatc cccgcgcagc
      661 ggcggcgcga ttcgctcgct gccacctggc aggcctggga caccgcgcgt cgggcggcaa
      721 acgggcgcct gcggcttgcg tgatggagag tcatgtctgt gtcgtttgat cctcgcgagt
      781 acgaaccggc cgccgacgag ctgagcggcc ggatcatcgc gatcaccgga gcaagcagtg
      841 gcatcgggcg cgcggtggcg atggactgtg cagcccacgg ggccagcgtc attctcatcg
      901 ggcgcaacgc gggaaaactg gaggccgtgc actcgcaaat cgaggcccgc ggagcaccgg
      961 aacccacgat tgccttgctc gatctggaaa aggcgctggc ggcggactac gaccagctgg
     1021 caagcgctgt ggtggagcgt ttcggacgcc tggacgggct actgcacaat gcggggatcc
     1081 tgggtaccct ttctcccatc gaacaccacg atgtgccgac gtggtgtcgc gtgctccacg
     1141 tcaatgtgac ggcagccttc gccctgacgc aggttctgct tccggcactg cggcgctccc
     1201 ccgatgcttc catcctgttc acctccagtt cggtgggtcg gcatggtcgt gcctattggg
     1261 gcgcctatgc cgtgtcgaag tttgcgaccg agggtctcgt gcaggtgctg gccgcggagc
     1321 tcgagaacac ctcctcggtt cgcgtcaatg ccgtcaatcc gggcaaggca cgcacgatga
     1381 tgcggcgcca ggcctacccg gcggaagatc tcaacagcct tccgccgccg gaaacgctga
     1441 cgggaccgta cattgcgctg ttgggtccgg gcagccgcgg cgtcacaggg atgagcttcg
     1501 actgtcagcg ggactagcca gaagatcttc ccgctcctcg tgagtcagct cgcggaagtg
     1561 tccgcgcggc agatgcggat ccagcagcag cggcccgagc tgtgtgcgca acacgcggct
     1621 cacgagagcg ccttgacgct cgaagagctg tcttacctgc ttgccactcg caccgagtgc
     1681 cgcgaaggcg taccagcggt tcgagccctc gccgccggcg ggctcacagc gctcgaccgc
     1741 gagactcacc ccgctatcga gctgccccgc cacaatgctg tcgagctgcc gtgttgaaag
     1801 ctcgccatgt acgcgcacgc tgaactcgct tgtcagtcgt ttgacgcagc gttgcagcct
     1861 ggaagccagc tcaccgtcgg aggtcaccag ctccagccct ccatcgatgc gcggcatcgg
     1921 actgatcgcc atgaaccgcc gccctgcccg tttcggcaat ctctccagca gcgcagtccc
     1981 ggagccctcg ggcggcgcgc cctcgcgagc ggcccccgcg ccgcgccgcg ccgggggctg
     2041 caaaggctcg ccgggtgagc ggtggcagag aaaaatccgc tccgtctcgg gggccccccg
     2101 ctggcggatc agtcgtccgt cgagccgcac ctgatctcca gggccgacgc gcacgccgag
     2161 tgcggcgagc tcgccgttga tggtgattcg accagcccgg atccagctct cgacttcgcg
     2221 ccgggaagcc agtccggcgc gcgcgagtac cttctgcagc cgctcgggtc cgccaggtcg
     2281 gtcagcgcga tgggagccgg cgtcgggccc ggaacgggga cgcatcggcg ctccgtgtca
     2341 gtcgtcgagc tgacgcggcc cggccacgag ctcgctcgaa tcgggcgctg ccgccgacag
     2401 ctcctcgtca tcgccggcat cctcgtcact gctcgtcaga tccgcgtcga ggtcagtcgt
     2461 ggggacggga cccggctcgg cggcgggctc gcctgagacc agctgcgcga cgaccgcctc
     2521 gggcaggtcg aactgcacct tgatgtcatc gagcgccttc agctccgcca gcggcggcag
     2581 ctcatccagc gtgcgcagcc cgaagtaatc caggaaatcg cgcgtcgtgc ccagaagctc
     2641 gggccggccc ggcacatccc gatggcccac gacgcgaacc cagttgcgct cgatgatcgt
     2701 cttcacgatg ttgggattga ccgcgactcc gcgcaccgcc tcgatctcgg cgcgcgtgat
     2761 cggctgtcga taggcgatca acgccagcgt ctcgagcagt gcgcgcgagt agcgcggggg
     2821 gcgctcgggc cagagccggg agatctcgtt cgccagctcc cggcggacct gaatgcggaa
     2881 tccggcggcg gtctccttga gctcgattcc gcggttgccg tactcctcag ccagagtctc
     2941 cagcgcctcg cgcacctcgg cagtcgtcgg gcgcacgccc tcatcgaaga gctcgaccag
     3001 ctcggccggg tgcagcggct tgccggccgc gagcagcgca gcctcgacta cattgcgcac
     3061 ttgcgactca ttcatgagtg tcctccgcgg gggaaacagg cggctctgcg accggcagct
     3121 cctgctcagc gaccagctcg ccggcactgg cttccacggc ctccggtgcg acccggtcct
     3181 cgttgtccgc tacgatgcgc agtacgcggt taggcgcagc tgcgcgcaca tgcagcggtg
     3241 caaaaggctc cgcctggacg atgtcgatca gaccttcgcg catcagctcg aggatggcga
     3301 cgaaggtgat cgtgaccccc atgcggccct cctcggcccg gaacaggcgc gcgaattcta
     3361 cgaaactcga atgctccagt gtcgacagga tgtcgcccat gcgctcgcgc accgacaggc
     3421 gctcgcgctg gatatggtga tgggcgaaca tctccgcgcg caccaccact tccttgaagg
     3481 cgatgagcat ctcctgaagg gtgatctgcg gcagcagtcg cacgaccttg cggtcgcgca
     3541 gctcggccga tgtcgtccag acatcccgct cgagccgtgg cagggcatcg ataccctccg
     3601 ccgcgcgctt gaatcgctcg tactcctgca ggcgccgcac gagctctgcg cgcgggtcct
     3661 cttcgatgcc ctcgggatcg agactggagc gcggcagcag cattcgcgat ttgatctcgg
     3721 cgagggtcgc tgccatgacc aggtactcgc cggcgagctc gagctgcagt tccgtcatca
     3781 gctcgatgta ctgcatgtac tggcgggtga tctcggcgag aggaatgtcc agaatgttga
     3841 gattctgccg ccggatcaga tacagcagca gatcgagggg gccctcgaag gcctcgagga
     3901 agatctccag cgcctggggc ggaatgtaga gatcccgcgg catctgcgtc acgggctcgc
     3961 cgttgacgac cgcgaagggc atctccacct gctgcggtga gggctccgcg gcggccgcgg
     4021 ggggttgggg cgccgtcggc gccgccggtg tgttggacac cacgatcttc aacttcaagt
     4081 gcgcgcctcc ggccgcactg gctcagtcag cccgcacgtg catcgcccgc cgtacctcat
     4141 cgagtgtctc ccgggcagca tcgcgtgcct tttccgcccc ctccgctacg ataccgcgca
     4201 cgagctccgg attctcctcg aattccgccg cgcgtcgccg catgttgccg atctcctcga
     4261 cgatccgatc gatcacgggc tgcttgcaat ccaggcagcc gatgccggcc gtgcgacagc
     4321 cttcgttgac ccacttgcgg gtcgactcat cggaatagat gcggtgcaga tcccacaccg
     4381 ggcacttctc gggatcgccc gggtccgtgc gccgcacccg tgccggatcg gtcttcatgg
     4441 cgcgcagctt ctgggtgacc acctcagggt cctcccgcag tccaatggtg ttgccatagt
     4501 acttggacat cttgcggcca tcgagacccg gaaccttcgg ggtggcggtg agcagcacct
     4561 gcggctcggg caggatcgtg acaccggtgc cctcgagaaa gccgagcagg cgctcacgat
     4621 cggcgacggt gatccggtta ttgctctgga cgagcgcgcg cgcccgctcg agagcctgca
     4681 cgtcgccgga ctcctggaac ttgcgccgca gctgcttgta ggccgtcgta ttgcggcccc
     4741 ccagactctt caccgctcgc tcggccttcg cctcgaaatc cggctcgcgt ccgtacagat
     4801 ggttgaagcg ccgcgcgatc tcacgagtca tctccacatg ggcgacctga tcctcaccca
     4861 ccggcacgaa cgcggcgcga tacatcagga tgtcggcgct ctgcagcagc ggatagccga
     4921 gaaaaccgta ggtggcgagg tccttctcct tgagctgctc ctgctggtcc ttgtagctgg
     4981 gcacgcgctc gagccagcca agcggggtca ccatcgacag cagcagatgc agctcggcgt
     5041 gctccggcac gtgcgactgc acgaacagtg tcgagacacc cggattcaaa cccgccgcga
     5101 gccagtcgat cagcacctcg cggatctgct cttgaagctg cgaagtatcc tcgtaccccg
     5161 tcgtgagcat gtgccagtcg gcgacgaaga agaagcactc gtactcatac tggaggttga
     5221 cccagttacg cagtgcaccg tggtagttgc ccagatgcag ccgcccggtg ggccgcatcc
     5281 ccgaaagcac tcgccgcgcc ggggcggtgg cgctcacttc attcctcgaa cgagactagg
     5341 caatggcgtc ctgaagctga ttgggagggg cggaccgatt atagcgcagg cgcccgagcg
     5401 gccccggggc cgagcagcgg agccaggctg cccttgcctg cgcgcacgat caccggcggc
     5461 gaggaggaaa gatcgaccac cgtcgtcggc tcgatcccgc agtcgccccc atcgagcact
     5521 gcatcgatct catgttcgag ccgttcctgg atgtcgcgcc catgagtcaa aggcagcgcg
     5581 tcacccggca gcatgagggt ggagctcatc agcggctcgc ccaactcggt cagcagcata
     5641 tgaggaaccg gatgcgccgg gatgcgcacc ccgatcgtgc gcctgcgctc atgttgcagc
     5701 cggcgcgggg tctcgcgcgt ggcgtgcagc aggaaggtgt agggaccggg tgtgcacact
     5761 ttcagcagac gaaactgcca ggtgtccaga cgggcatagc gccccacgtc ggtgaggttc
     5821 gcgcacacca gcgtgaagtg atggtggcgg tcggcgcgac ggatcgtacg cagccgctcg
     5881 agcgcattct tgtcattgat gtggcagccg agcgcatagc acgaatccgt cgggtaggcg
     5941 atgaccccgc cacggctgac gatctcggca gcccggcgca cgagccgcca ctgcggattg
     6001 acggggtgca ggccgaagta ttcgctgggc atggactgag taaaaccggc tatcatacgc
     6061 ggccctttag ccgcggttag caccttaagc cgccacattt tcaagtcatg caggctctgc
     6121 tggagttagc gcctctaatc gcgttcttcg tcgcttaccg ggtatggggg ctgtacgtgg
     6181 cgaccgccgt cctcatgggc ggcatgctcg tactggtcat caccgactat ctgcgcctgc
     6241 gccgcatccc tgcgatgcat ggactcagca ccgtcctggt gctggtcttc gggaccgcca
     6301 cgttggtgct gcacaacctg cgcttcatcc agtggaagcc gacagtgttc ttctggctcg
     6361 ccagcctcgc tttcctcgca agtttctggc tcggaaagca gaccttgacg cagcggctgt
     6421 tgggggcggc gcttgcgggg gaggactcca aggtgtccca gggcacctgg aaacggctga
     6481 atacgctgtg ggtgctcttc tacgcgttgc tgggcgctct caacctcctc gtggcattca
     6541 acttcagcga gcatgcgtgg gtggatttca aggtcatcgg gcttccggtg gtgaactttc
     6601 tgttcgccgg tgcccagatc gcctggctga tgcgccgcgc gccttcgggc tcaggtgagc
     6661 cctcccctca ggcctgaatg cacactgctt cgagggacgc gcttcgcatg gcctcaatcg
     6721 acatcgaaca gctgcgaact gcgctggagc gcggcctgaa tcccctgtcg ctcgagatca
     6781 tcgatgacag caggcaccac gccggacatg ccggggcgaa ggaaggggga cacttccgca
     6841 tccgcgtcat cgccagcgcc tttgcgggac gctcgcaact cgagcgccac aggctggtct
     6901 atgcagccgt cgcgccgctc atgggccatg gtgtccacgc cctcagcatc gatgcccatg
     6961 cacctgaaga aaacacttaa gtaccacgtc taactaactg agaatacaca tgagttatta
     7021 ccgacttctg ctcgtggcca ccgccctcgc cctggtcgct tgccagccca agggcgccag
     7081 caccgctgcc gctccggcag ctgcggacaa gtcacccgag gtggcttccg tcaacggcac
     7141 ggtgatcacg cgcaacttct acgatgagta catcaaagcc gtgaccggca aatcctcggg
     7201 cgatctcacg cccgagcagc gcaatgatgc tctcgatcgc ctgatccgga tggagctggt
     7261 gagtcaacag gcggtgaagg acggtgtcga tcgcacaccc gacaccgcgg cgatgctgga
     7321 gttgactcgt ctcaacgtgt tgcagcaggc ggtgtccgag cagtatctga aggatcgcaa
     7381 gccgaccgag caggagctgc gcgcagaata cgagacgcag ctcgccgggc tatccaagca
     7441 ggagtatcac gccaagcaca tcctggtcgc gacggagccc ttcgcccaga aggtcgtgca
     7501 gcagctggag aagggcgcaa agttcgaaga cgtcgccaag cgcgaatcga tggatgcctc
     7561 caaggacaat ggcggcgatc tcggctggtt cacgcccgat cggatggtca agccattcgc
     7621 cgatgcggtg gtggcgctca agccgggcga gtacacgcat acgccggtgc acactgacta
     7681 cggctggcat gtgatcgagc tggtcgaggt gcgggacctg caaccgcctg ctttcgataa
     7741 cgtgcgccag cggctcgagc aggtcgtgca ggccaagaag ctcaaggcct acaccgatga
     7801 gctgctgaag ggcgcgaagg tcgacaagca gctcgatcag aagaagagct cctgaggcga
     7861 cgggcccgcg gctgcttggc cgcgggctct gccggcccca gctcccggct cagctctacc
     7921 gatccgttca cgccgagccg cgctgatcgc tcagcgcgcg caacgcggcc ccaccgtctc
     7981 gcttgggccc gggggcgcaa ccccaatact tgttcccgac ggccgcgatt taaccgttta
     8041 tgtcagccag cgcggccggc gcgcgttttg ctggtagaac tcgcgaaatc ccacgctcag
     8101 gctgggaaac ttgaacgggg gtggattccg gaggtctaat ctgtcaggcc atggggccgt
     8161 tgcggacgct cgaaaagagc gttcgcgtcg gagtgaacat atgaacgcaa agggtaaata
     8221 ttcgatctgg gcagcgggtg ctctggccgc gcttctcgcc gccagcacgc cggcgtttgc
     8281 gcagaaccat ggcggtggcg ggggtcacgg cggtggtggt ggcggccact tcggtggtgg
     8341 cggtcacgcc tcgatgggcc acttctccgg aggtggggcc cggatgggcg gcgcgcatta
     8401 cgcgccccgg gcaggagcct acgcggggca tgggtacgct ggtgcgcatg tagggtccgg
     8461 attccacggc acaacggtca gctccgcggc ttggcatggc ggctcgcgcg cgggctgggc
     8521 cggtgggcat gccgggtggg gcggacgtgc gggctggggc cgaccgggat acggacatgg
     8581 ctttcaccgt ggattttggg gcggtggcta ctggcatggc gg
//
LOCUS       DASRHN010000438        11808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U10085
            k141_2469418, whole genome shotgun sequence.
ACCESSION   DASRHN010000438 DASRHN010000000
VERSION     DASRHN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963613
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,563
            CDSs (total)                      :: 3,528
            Genes (coding)                    :: 3,507
            CDSs (with protein)               :: 3,507
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469418"
                     /isolate="SMAG_U10085"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VFB37_17775"
     CDS             complement(<1..898)
                     /locus_tag="VFB37_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO24347.1"
                     /translation="MSTRSRSDGQSAPAAAAEAPILRIENVTRKFGDFVAVNDVSLEV
                     QRGEIFCLLGGSGSGKTTLLRMLAGFDTPTQGRIYIDGQEMSGIPPYERPVNMMFQSY
                     ALFPHMSVEKNVAFGLEQEHLGRDEVRRRVGEILAIVKMTPFMARKPHQLSGGQRQRV
                     ALARALVKRPKLLLLDEPLAALDRKLREHTQFELINIQEQLGVTFVVVTHDQEEAMTL
                     SSRMGVMDHGHIVQVGTPTDIYEFPATRFVADFIGSVNMFEGRLIEDEPDFVRIQCDD
                     LGGVVHVNHGISSAPGATVWVAV"
     gene            complement(915..2072)
                     /locus_tag="VFB37_17780"
     CDS             complement(915..2072)
                     /locus_tag="VFB37_17780"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZO24348.1"
                     /translation="MSSARAAISMKHWLLPLCLAAALAACGGGSQSTHRSGSSAGSQP
                     EEKVLNVYNWSDYIEPGVLEDFHKQTGIKINYDVFDTNEVLETKLLAGHTNYDVVVPS
                     GPFLERQIQAGVFQKLDKSKLPNLKNLDPQVLKQLEMYDPGNAYSVPYMWVTSGPGYN
                     VAKIHERMADAPLDSLRMLYDPAVVSRFADCGVSVLDAPSEVIGTVLIYLGKPGDSES
                     PQDLQAAENVLMSIRPYLRYVNSSRYIEDLANGETCLALGWSGDVKQARDRAHDAGKG
                     IEIRYSIPRNGTILNFDMLAIPADAPHPQNAHLFINFLLDAKIAARNSNLVKYANANL
                     ASQPFLDEAVKSDPNIYPPPQIRAKLVPEPPRSLTYQRLLTRAWTRFKTGK"
     gene            complement(2122..3459)
                     /locus_tag="VFB37_17785"
     CDS             complement(2122..3459)
                     /locus_tag="VFB37_17785"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZO24349.1"
                     /translation="MSEIQQFFRDHGISEVEAIIPDMAGIARGKIMPAAKFAEDEGMR
                     LPESVFLQTVTGDYPLETGEAMSPAEIDIVLKADPKTVRRVPWAAEPTAQVIHDCFYS
                     DGRRVSMAPRHVLRNIIEMYAQRGWEPVVAPELEFYLIEQNKDSDYPLRPPVGRSGRA
                     EPGRQSYSIAAVNEFDPLFDDIYQFCEDQDIEIDTLIHEDGPAQMEINLIHGYPLDLA
                     DQAFLFKRTAREAALRHKIYATFMAKPHAKEPGSAMHIHQSVVDTKTRRNIFSNGDGT
                     PSNLFFSHIAGLQKYMPAAMALFCANVNSYRRLTRYLSAPINVHWGYDNRTAGLRVPM
                     SSADARRVENRVGGADANPYIAIAASLACGYLGMVEGLQPTDPITGSAHDLPFGLPRS
                     LDEALRELRASEPLVRLLGEPFVSAFTIVKEAEYEVFLQVISSWEREHLLLNV"
     gene            complement(3521..4282)
                     /locus_tag="VFB37_17790"
     CDS             complement(3521..4282)
                     /locus_tag="VFB37_17790"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZO24350.1"
                     /translation="MAARPLIGIPADRHIIGPHPFHAVGEKYVRAVLDAAEGLPLLIP
                     ALADELGMDDLMRRLDGILFTGSPSNVEPLHYSGPPSEPGTLHDPARDATTLPLIRKA
                     VAGGIPVLGICRGFQEMNVAFGGSLHQKVHEVQGKLDHRDDGSLPLEVQYGPAHDVLL
                     EPAGVLRALAASDRVRVNSLHSQGIDRLGCGLAVEARAPDGLVEAFRVEGARRYALAV
                     QWHPEWKVMESQFSRAVFAAFGAACREYAHAMRGR"
     gene            4470..5765
                     /locus_tag="VFB37_17795"
     CDS             4470..5765
                     /locus_tag="VFB37_17795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO24351.1"
                     /translation="MAAARHVASYYAASSNAAPPRAPLGASVDCDVCVVGGGIAGCST
                     ALHLAERGYSVVLLEENLIGWGASGRSGAQAIHGVAAGQAKLERLIGSAAARLVWDVS
                     VEGLVLMRELIARHEIACDWVSGHLLTAIKPRHELELRHELEELHGRYGYPHVRYMPR
                     EEVRSLVATDRYLGGLYDAQSGHLHPLNYTLGLAAAAERSGTRIFEGTRALAFASDSN
                     TATARTRVRTPQGEVTCRYLVLCGNVYLRDTAPAIASKIMAVATYIVASERLGAERAR
                     KLILNNSAVHDMNWVLDYFRLSADHRLLFGGRVNYSGLSSFDAPTATRARMLRVFPQL
                     ADVGIEYAWGGEVDITLNRAPHFGRLAPNVYFLQGFSGHGIALTGIAGKLVALAIAGT
                     AERFDVFARIPHGNFPGGAALRRPTLVLAMLYYRLKDLL"
     gene            complement(5820..7982)
                     /gene="rlmKL"
                     /locus_tag="VFB37_17800"
     CDS             complement(5820..7982)
                     /gene="rlmKL"
                     /locus_tag="VFB37_17800"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HZO24352.1"
                     /translation="MRQFRASSPRGLADLLARELTIFGADNLRERSAGVTFSGDLRVA
                     YRACVGSRIANRIFLELAAFEARDAQEFYGAARELNWAAELAPDATLACEFTGKHPNI
                     THTHFGALKLKDAIVDAVRDVRGTRPDVALERPGLRVHAHANGTRITLSLDLSGESLH
                     RRGYRATAGEAPLKENVAAGMLLRAGWAEMAAEGASFLDPLCGSGTLVIEAAWIAARR
                     APNLGRQYFGFLGWRGHDPTLWSEVLQEARERVGTRLPQPAVIRGTDRDPGAIRDARA
                     NAARAGISELVQLEVGLLADARPPVSNGNGLLAVNPPYGVRLEDHDQALSLHRELGGV
                     LRGRFQGWQAAILIGAPQLGRELGLRASRTHVLWNGGIECRLLRLRVDPASEREPGRL
                     GRADSAVRDSPGARMFANRLAKNLKRLQSWAARSGISCYRLYDADMPEYAFAIDRYRT
                     IDPPMEWLYVQEYAAPAEIEPESVRRRRQEALSVLPEVTGVPRERIRVRTRRRMRGSD
                     QYGKVHEEGAFHVVEEGGLKFQVNFDEYLDTGLFLDHRLTRERLRSHARGKRFLNLFA
                     YTGTATAYAAAGGATASTSVDLSRTYLDWTRRNLTLNDVSGSQHELIQADCREWLQPG
                     SRGGARYELILLDPPTFSNSKRMQGILDIARDHPALIDACMSLLAPGGLLIFSTNAQR
                     FRLEPALGTRYRIRDISAATLPEDFERNPRIHHCFEIG"
     gene            complement(7997..9496)
                     /locus_tag="VFB37_17805"
     CDS             complement(7997..9496)
                     /locus_tag="VFB37_17805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZO24353.1"
                     /translation="MAAKPESISWHERARALEIHTKAFINGNYADAASGATFDTVNPG
                     TGKLLARVAAGDAEDIDRAVGAARTAFRKGVWANVAPAKRKQVLKRFAELILKNGEEL
                     ALLETLDMGKPIGVSLNVDIPSAARCIEWYAEAIDKIYDEVAPTGPGSLAIVTREPMG
                     VVGAIVPWNYPLLMAAWKIGPVLAAGNSLVLKPSEKSPLTAIRVAQIALEAGIPEGVF
                     NVVPGLGAAAGKALALHMDVDCIAFTGSTATGRSIMQYAGQSNMKRVSLECGGKSPNI
                     VMADYPDLDRAATAAAYAIFDNQGEVCSAGSRLLVQESVKDRVLEKVQAIGKSLQPGD
                     PLDTDTKLGAIVDDLQMKRVLGYIESGRRDGATVRFGGRRVREDSGGYFVEPTVFDSV
                     RPSMAIAREEIFGPVLSTITFKTVDEAIEIANDVIYGLVAAVWTRDVTTAHRTAKALR
                     AGTVFVNCYDADDITVPFGGFKQSGIGRDKSLHALEKYTELKTTWIDLS"
     gene            complement(9532..10074)
                     /locus_tag="VFB37_17810"
     CDS             complement(9532..10074)
                     /locus_tag="VFB37_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO24354.1"
                     /translation="MDVGSRLRGIRTTFGLSQRELARRAGVTNGLISLIEQNRVSPSV
                     SSLKKVLDGVPMSLAEFFTLDLSAPPQTFFTRDELVELGNTQVSLRLVAAQRPSRQLT
                     ILHERYAAGSGTGDEMIVHRGEEGGVVIRGRVELTVGGASRVLGPGEAYYFASQLPHR
                     FRNAGREPCEIISASTPPSF"
     gene            10276..11589
                     /locus_tag="VFB37_17815"
     CDS             10276..11589
                     /locus_tag="VFB37_17815"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZO24355.1"
                     /translation="MPSAADLEAHWMPFTPNRQFKARPRMFARASGMYYWTADGRQVL
                     DGVAGLWCVNAGHGRTEISEAVATQMRTMDYAPTFQMGHPAAFEAANALVAIAPAGLD
                     HVFFTNSGSESVDTALKIALAYHRVRGDAARTRLIGRERAYHGVGFGGISVGGISPNR
                     RMWSSALIPGVDHLGHTHDLSRNAFSRGEPAHGVQLADDLERIVALHDASTIAAVIVE
                     PIAGSTGVLIPPRGYLKRLREICDRHGILLILDEVITGFGRTGSAFASQEFGVTPDLV
                     TLAKGLTNGCIPMGAVLAKKAIYDAFMQGPEGSIELFHGYTYSAHPAACAAALAAVGI
                     YQREGLLTRARTLSDLWADAAHSLRGLPHVIDVRNYGLIAGIELDPIPGKLGSRAHEV
                     FLRCFERGLLIRQTGETLALSPPLIIERSHIDQIFTTVKEVLRTM"
BASE COUNT         2025 a         3872 c         3784 g         2127 t
ORIGIN      
        1 gcacggcgac ccagaccgtg gcgcccggcg ccgagctgat cccatggttg acgtgcacca
       61 cccccccgag gtcatcgcac tggatccgca cgaaatccgg ctcatcctcg atcagccgcc
      121 cttcgaacat gttcaccgag ccaatgaagt cggcgacgaa gcgcgtcgcc ggaaactcgt
      181 agatgtcggt cggcgtgccg acctggacga tatgcccgtg gtccatgaca cccatgcgcg
      241 aagagagggt catggcttcc tcctgatcgt gcgtcaccac cacgaaagtg accccgagct
      301 gctcctggat gttgatgagc tcgaactgcg tgtgctcgcg caacttgcgg tccagcgcgg
      361 ccaggggctc atcgagcagc agcagtttcg gccgcttcac cagcgctcgg gccagggcca
      421 cccgctggcg ctgcccgccc gagagctgat gcggcttgcg cgccatgaac ggggtcatct
      481 tcacgattgc gagaatctcg ccgacgcggc gccgcacctc atcgcgcccg aggtgctcct
      541 gttccaggcc gaaagccacg ttcttctcca cactcatatg ggggaagagc gcgtaggact
      601 ggaacatcat gttgaccggc cgctcgtagg gcgggatccc gctcatctcc tggccgtcga
      661 tgtagatccg gccctgcgtc ggggtgtcga atcccgccag catgcgcagc agcgtcgtct
      721 tgcccgagcc cgagccgccg agcaggcaga agatctctcc gcgctgcacc tccaggctca
      781 catcgttgac ggctacgaag tcgccgaatt ttcgcgtgac gttttcgatg cgaagaatgg
      841 gagcctcagc ggccgcggct ggggcactct gcccatcgct cctcgatctg gtgctcacgc
      901 agcagcggcc gatgtcactt gcccgtcttg aaccgggtcc atgcgcgcgt cagcagccgc
      961 tggtacgtca gggaccgagg cggctcgggc acgagcttcg cacggatctg cggcggcggg
     1021 taaatgttgg ggtcgctctt caccgcctcg tcgagaaaag gctgggatgc caggttcgca
     1081 ttggcgtatt tcaccaggtt cgagttgcgg gcggcgatct tcgcatcgag caggaaattg
     1141 atgaacagat gcgcattctg tggatggggc gcatcggcgg ggatggcaag catgtcgaag
     1201 ttcaggatgg tgccattgcg tggaatggag tatctgatct cgatgccctt gccggcatcg
     1261 tgcgcccggt cacgcgcctg cttgacatcg ccggaccaac cgagcgccag gcaggtctca
     1321 ccgttggcga ggtcctcgat gtagcgcgat gaattgacgt aacgcagata gggtcggatc
     1381 gacatcagca cattctcggc cgcctgcagg tcctgcggcg attcgctgtc gcccggttta
     1441 cccaggtaga tgagaaccgt gccgatgact tccgagggcg catcgaggac cgagactccg
     1501 caatcggcga acctggacac gacggcgggg tcatacagca tgcgcagact gtccagcggc
     1561 gcatcggcca tgcgctcgtg gatcttcgcc acgttatagc caggtcccga ggttacccac
     1621 atgtagggga cgctgtaggc attgcccgga tcgtacatct cgagctgctt gagcacctgc
     1681 ggatccagat tcttgaggtt gggcagcttg gatttgtcga gcttctggaa gacgcctgcc
     1741 tggatctgcc gctccagaaa cggcccggac gggaccacga cgtcgtagtt ggtatggccg
     1801 gcgaggagct tcgtctcgag cacctcattg gtgtcgaaca cgtcgtagtt gatcttgatg
     1861 cccgtctgct tgtggaaatc ctccagcacg ccgggctcga tgtagtcgga ccagttgtag
     1921 acgttgagga ccttctcttc cggctgtgat ccggccgagc tgcccgatcg gtgcgttgac
     1981 tggctgccgc cgccgcaggc agccagtgcc gctgccaggc acagcgggag cagccagtgc
     2041 ttcatggaga tcgccgcacg agcgctactc atctgcttca tctccaggtg aacggttgcc
     2101 cctcgcagct ttatatagca ttcacacgtt cagcagcaga tgctcgcgct cccaggagct
     2161 gatcacctgc aggaatacct cgtactcggc ctccttgacg atggtgaacg ccgagacgaa
     2221 cggctcgccg agcagtctca cgagcggctc ggaggcccgc agctcccgta gcgcctcatc
     2281 gagtgagcgg ggcagcccga acggcaggtc atgcgcgctg cccgtgatcg gatcggtggg
     2341 ctgcaggccc tccaccatgc ccaggtaacc acatgccagc gaagcggcaa tggcgatgta
     2401 gggattggcg tcggctccgc ctacacggtt ctccactcgg cgggcatcgg cactcgacat
     2461 cggtacgcgc aagccggccg tgcggttatc gtatccccag tgaacattga tcggtgccga
     2521 caggtaccgt gtcagccggc gatacgaatt cacattggca cagaagagcg ccatggccgc
     2581 aggcatgtac ttctgcagcc cggcgatgtg cgagaagaac aggttcgagg gcgtcccgtc
     2641 gccgttgctg aagatattgc ggcgcgtttt cgtgtccacc acgctctggt ggatgtgcat
     2701 ggcgctgccc ggctccttgg cgtgcggctt cgccatgaag gtggcgtaga tcttgtgtcg
     2761 caacgcggcc tcgcgtgcag tgcgcttgaa gagaaacgcc tgatcagcca ggtcgagcgg
     2821 atagccgtgg atgaggttga tctccatctg cgccggacca tcctcgtgaa tcagcgtatc
     2881 gatctcgatg tcctggtcct cgcagaactg gtagatgtca tcgaacagcg gatcgaactc
     2941 gttgacggcg gcgatgctat aggactggcg cccgggctcg gcgcgtccgg atcgacccac
     3001 cggcgggcgc agcggataat ccgaatcctt gttctgctcg atgagataga actccagctc
     3061 cggagccacg accggctccc agcccctttg ggcatacatc tcgatgatgt tgcgtagtac
     3121 gtgacggggt gccatggaga cccgccggcc atccgaatag aagcagtcgt ggatcacctg
     3181 tgcggtgggc tcggctgccc agggcactcg gcgaacggtc ttcggatcgg ccttgagcac
     3241 gatgtcgatc tcggctggac tcatcgcctc accggtctcg agcgggtagt cgcccgtgac
     3301 ggtctgcagg aagacgctct cgggcaggcg catgccctcg tcctccgcga acttcgccgc
     3361 cggcatgatc ttgccgcgcg cgatgcccgc catgtcggga atgatcgcct cgacctcgga
     3421 gatgccgtga tcgcgaaaga actgctgaat ctcgctcacc ataggaatga ccttatttca
     3481 tggccggggc ccgaagggag cccaccaggg gcgcccgact tcatcgaccc cgcatcgcgt
     3541 gcgcatattc gcggcaggca gcgccaaagg cggcgaacac agcgcgcgag aactgactct
     3601 ccatcacctt ccactcggga tgccactgga cggcgagcgc atagcgcctc gccccctcga
     3661 cccgaaaggc ctcgaccaga ccgtccggcg cccgtgcctc gaccgctaag ccgcagccca
     3721 gccggtcgat gccctgcgag tgcagagagt tgactcgcac gcgatcgctc gcagccagcg
     3781 cccggagcac tccggcaggt tcgagcagca cgtcgtgggc gggcccgtac tgcacttcca
     3841 ggggcaggct cccgtcatca cgatgatcga gcttgccttg cacctcgtga accttctgat
     3901 gcagcgagcc gccgaaagcg acgttcatct cctggaaacc acggcagatc cccagcaccg
     3961 gaatcccgcc tgcgacggcc ttgcggatca ggggcagcgt ggtggcatcc cttgccggat
     4021 cgtgcagtgt gccgggctcg ctcggcggcc ccgaatagtg cagcggctcc acgttggatg
     4081 ggctgccggt gaagaggatc ccgtcgagcc gacgcatgag atcgtccata ccgagctcat
     4141 cagccagcgc cgggatcagc agcggcagcc cctcggccgc atccagcacc gcgcgcacgt
     4201 atttctcgcc gaccgcatgg aacggatgcg ggccgatgat gtgacggtct gcaggtatac
     4261 cgatcagtgg gcgagcggcc atgacgcttc tgcaggcagc gtctatttta ctaaacagac
     4321 ccgcccggcg gcttctccaa agccgatatt agaacacagc tcggggtcgg gaatctgcct
     4381 gggaggattt gccccgattg tgtgcggctc gctatcctgc cggcgcgctg cgcccgtccc
     4441 ggcgcgtcta aatatcttaa acacctcgca tggctgccgc gcgccacgtt gcctcctatt
     4501 acgccgcatc gtcaaatgcg gcgcccccgc gtgccccgct aggcgcctct gtcgattgcg
     4561 acgtatgcgt agtcgggggt gggattgcag gctgctcgac ggcgctgcat ctggccgagc
     4621 gcggctactc cgtggttttg ctggaggaga atctcatcgg ctggggggcc tcgggtcgca
     4681 gcggagcgca ggccatccac ggggtggcag cgggtcaggc gaagctcgag cgactgatcg
     4741 gatccgcagc tgcccgcctg gtgtgggatg tcagcgtgga agggcttgtg ctgatgcgcg
     4801 agctgatcgc acgccacgag atcgcctgtg actgggtgag cgggcatctg ctgaccgcga
     4861 tcaagccccg gcacgagttg gagttgcgcc acgagctgga ggagttgcac ggcaggtacg
     4921 gctacccgca cgtgcgctac atgccgcgcg aggaggtccg ctcgctggtg gccaccgacc
     4981 gctacctcgg tgggctgtac gatgcccaga gcggtcacct gcaccctctc aactacactc
     5041 tgggacttgc tgccgccgcg gagcgcagcg ggacacggat cttcgaagga actcgcgcgc
     5101 ttgcgttcgc gagcgactcc aacactgcca ccgcgcgaac ccgggtgcgc acgccgcaag
     5161 gtgaggtcac gtgccgctac ctggtgctct gcggcaatgt ctacctgcga gataccgccc
     5221 cggcgatcgc atcgaagatc atggcggtcg ccacctacat cgtggcaagc gagcgcctgg
     5281 gtgccgagcg ggcccgcaag ctgatcctga acaactcggc cgtgcacgac atgaactggg
     5341 tgctggacta cttccggtta tcggccgatc accgcctgct ttttggaggg agggtgaact
     5401 actcggggct cagctcgttc gatgctccga cagccacgcg ggcccgcatg ctgcgcgtgt
     5461 ttccccagct cgcagatgtt ggcatcgagt atgcatgggg cggcgaggtg gacatcacac
     5521 tcaaccgtgc gccgcacttc ggccggctgg cacccaacgt ctattttctg cagggcttct
     5581 ccggtcatgg catcgccctc accggtatcg ccggcaagct ggtcgcgctg gcaatcgcgg
     5641 ggacggccga gcgcttcgac gtgtttgcac gcattcccca cggcaacttc cccggcgggg
     5701 ccgcgctgcg ccgcccgaca ctcgtgctgg cgatgctcta ctatcgcttg aaggatctgc
     5761 tgtgagcgct gcggggcgcg ctgccgagat gcgggcgggg cgtcccgggc gtaagcgcct
     5821 cagccgatct cgaaacaatg atggatgcgg ggattgcgct cgaaatcctc tggcagtgtc
     5881 gccgcggaga tgtcgcggat gcgatagcga gtgccgagcg cgggctcgag cctgaaacgc
     5941 tgcgcgttgg tcgagaagat gagcagcccg ccgggcgcca gcaggctcat gcaagcatcg
     6001 atcagggcag gatgatcgcg cgcgatgtcc agaatgccct gcatccgctt cgagttcgag
     6061 aaggtcggcg gatcgagcag gatcagctcg tagcgggcgc cgccgcggct gcccggttgc
     6121 agccattccc ggcagtccgc ctggatgagc tcgtgttggc tgcccgagac atcattgagc
     6181 gtcagattgc gccgggtcca gtcgaggtag gtgcgcgaaa gatctacgga agtgctcgcc
     6241 gtcgccccgc ccgctgccgc gtacgccgtc gccgtgccgg tgtaagcaaa caggttgaga
     6301 aagcgtttgc cacgtgcatg cgagcgcaga cgctcgcgcg tcaggcgatg atcgagaaac
     6361 aatccggtgt ccaggtactc atcgaaattg acctggaact tcaagccacc ttcctcgacc
     6421 acgtggaagg cgccctcctc gtggaccttc ccatactgat cgctgccgcg catccggcgg
     6481 cgggtgcgca cgcggatccg ctcgcggggc acgcccgtca cctccgggag caccgataag
     6541 gcttcctggc gtcgccggcg aacggactcc ggctcgattt cggccggggc cgcgtactcc
     6601 tgcacgtaca gccactccat cggcggatcg atcgttcggt aacgatcgat tgcgaaggcg
     6661 tactcgggca tgtccgcatc gtagagccgg taacaggaga tcccgctgcg cgcagcccag
     6721 ctctgcagtc gtttgaggtt cttggccagc cgattcgcga acatccgcgc acccggagaa
     6781 tcgcgaacgg cggagtcggc tcggccgagc ctgccgggct cgcgctcgct cgccgggtcg
     6841 acccgcagcc gaagcagccg gcactcgatt ccaccattcc acaggacgtg agtgcgggag
     6901 gcacgcaggc ccagctccct gccgagttgc ggggcgccga tcaaaatggc ggcctgccag
     6961 ccttgaaatc gcccgcgcag tacgcctccg agctcgcgat gcaggctcag agcctgatcg
     7021 tgatcctcca accgcacgcc ataaggcgga ttgacggcga gcagcccatt gccgttcgag
     7081 accggtggcc tcgcatctgc aagcaagccc acttcgagtt gcacgagctc gctgatgccc
     7141 gctcgagcgg cgttggcgcg tgcatcgcga atcgcgccgg gatcacgatc agtgcctcgg
     7201 atgaccgcgg gctgcggcaa ccgtgtcccg acccgctcac gagcttcctg caacacctcg
     7261 ctccagagtg tcggatcatg tccacgccag cccaggaagc caaaatattg gcggcccaga
     7321 ttcggcgccc gccgggcggc tatccatgcc gcttcgatca cgagggtacc cgatccgcac
     7381 aggggatcca ggaacgaggc gccttccgcg gccatttcgg cccacccggc gcgcagcagc
     7441 atgcctgccg ccacgttctc cttcagcggc gcctcgccgg ctgttgcgcg atagccccgc
     7501 cgatgcagac tctccccgga gagatccaga gacagcgtga tccgggtccc gtttgcatgg
     7561 gcgtgcaccc gaagcccggg tcgctcgagc gccacgtccg gacgcgtgcc gcgcacatcc
     7621 cgaacggcat ccacgatggc gtccttcagc ttgagcgccc caaagtgagt gtgcgtgatg
     7681 ttagggtgct tgcccgtgaa ctcgcaggcc agagtcgcat cgggcgcgag ctcagccgcc
     7741 cagttcagct cgcgcgccgc accgtagaac tcctgcgcgt cgcgtgcttc gaaggcggcg
     7801 agctcgagga agatgcggtt ggcgatgcgt gagccgacac aggcccgata ggcgacccgc
     7861 agatcgccgc tgaacgtcac tccggccgag cgctcgcgca ggttgtcggc gccgaagatc
     7921 gtgagctcgc gtgcgagcag atcggcgagc ccacgcggag agctcgccct gaactggcgc
     7981 accccgacgc cgtcactcag gacagatcga tccaggtcgt cttgagctcg gtgtacttct
     8041 ccagggcatg gagcgacttg tcgcggccga tgcccgactg cttgaagccg ccaaagggca
     8101 cggtgatatc gtcggcgtcg tagcagttca cgaatacggt tccggcgcgc aacgccttgg
     8161 cggttcggtg tgcagtggtc acatcacgcg tccacacggc agccaccaac ccgtagatca
     8221 catcgttcgc gatctctatc gcctcatcga cggtcttgaa ggtgatcgtg gagagcaccg
     8281 gtccgaagat ctcctcgcgg gcgatcgcca tgctcgggcg gactgaatcg aacaccgtcg
     8341 gctcgacgaa atagccgccg ctgtcctcgc gcacccgccg tccgccgaag cggacggtgg
     8401 ccccgtcccg gcggcctgac tcgatgtagc cgagcacccg cttcatctgc aggtcatcca
     8461 cgatcgcgcc gagtttcgta tcggtatcga gcggatcacc tggctgcaag ctcttgccaa
     8521 tggcctggac cttctcgagc acccggtcct tgacactctc ctgcaccagc aggcgggagc
     8581 ccgcggagca gacctcgccc tgattgtcga agattgcata cgcagcggcg gtggcggccc
     8641 gatcaagatc cggataatcg gccatgacga tgttgggaga cttgccgccg cactccagcg
     8701 acacgcgttt catgttcgac tggccggcat attgcatgat cgagcgaccg gttgccgtcg
     8761 agcccgtgaa ggcgatgcag tccacatcca tgtgcagcgc cagggccttg ccggcggccg
     8821 cccccagtcc cgggacaacg ttgaagaccc cctccgggat gccggcctcg agcgcaattt
     8881 gcgccacccg gatcgcggtc agcggagact tctccgaggg cttgagcacc agcgaattgc
     8941 ccgccgccag caccggcccg attttccagg ccgccatcag cagcggatag ttccagggca
     9001 cgatggcgcc gacgacgccc atcggctcgc gggtgacgat cgccagcgag ccgggtccgg
     9061 tcggcgccac ctcgtcatag atcttgtcga tcgcctcggc gtaccactcg atacagcgag
     9121 cggccgaggg aatatcgacg ttgagactga cgccgatggg cttgcccatg tcgagtgttt
     9181 cgagcagcgc aagctcctcg ccattcttca gaatcaactc ggcgaagcgc ttgagcacct
     9241 gtttgcgctt ggccggcgcg acattcgccc acactccctt gcggaatgcg gtgcgcgcag
     9301 caccgactgc gcggtcgatg tcctcggcgt cgccggcggc gacccgggcc agcagcttgc
     9361 cggtaccggg attgacggta tcgaacgttg cgcccgaggc cgcatcggca taattgccgt
     9421 tgatgaaggc tttcgtgtgg atctcgagcg cgcgcgcacg ctcgtgccag gagatggatt
     9481 caggtttggc agccatgggt ctctctcgtg tccaggttcg ttgtcggaat atcagaagct
     9541 cggaggcgtg ctggcactga tgatctcaca gggctcacgt cctgcattgc ggaagcggtg
     9601 cggcagctgg ctggcgaagt agtaggcttc gccggggccg agcacccggc tcgcgccccc
     9661 taccgtgagc tcgacgcgcc cccggatgac tacgccgcct tcttctccgc ggtgaacgat
     9721 catctcgtcc cccgtgccgg aaccggcggc atagcgctcg tgcagaatcg tgagctggcg
     9781 gcttggccgc tgcgcagcga ccagccggag cgaaacctgc gtattgccca gctcgaccag
     9841 ctcgtcccgg gtaaagaacg tctgcggtgg tgcactcaga tcgagggtga aaaactctgc
     9901 gagggacatg ggcaccccgt caagcacctt cttgagagag ctgaccgagg ggctgacgcg
     9961 attctgctcg atcagcgaga tcagtccgtt ggtgacgccg gcgcggcgcg ccagctcgcg
    10021 ctgcgagagc ccgaatgtgg tccggatccc gcgcagccgg ctgccaacgt cgatgctcat
    10081 gagaaggccc cgtctgcgcg agtgtttaag attatcgaca agtgctgccg aaatctggtc
    10141 cggatggccc tgattgtcaa atttccttat catcgccagc ctccccctcc tacaggcgaa
    10201 tgctccatgg cccccgcgac ttccgaatcg ctgtcccctg ccggcgcccg cgcagggact
    10261 gccccggact cccccttgcc ctccgcggcc gatctggagg cgcactggat gccgttcacc
    10321 ccgaaccggc agttcaaggc ccgcccccgc atgtttgcgc gggcgtccgg catgtactac
    10381 tggaccgccg acggacgaca ggtgctcgat ggtgtcgccg gtctatggtg tgtcaatgcc
    10441 ggacacggac gcacggagat cagcgaggcg gtcgccacgc agatgcggac gatggactac
    10501 gctccaacgt tccagatggg ccaccccgcc gccttcgagg ccgccaacgc cctggtcgcc
    10561 atcgccccgg ccgggctcga ccatgtgttc ttcaccaact cgggatcgga atcggtcgac
    10621 accgccctga agatcgccct ggcgtaccac cgcgtgcggg gcgatgcggc gcgcacgcga
    10681 ctgatcggcc gagagcgcgc ttatcacggc gtcggcttcg gcggcatctc ggtcggtgga
    10741 atctcaccaa accgcaggat gtggtcctct gcgctcattc ccggcgtcga tcacctcgga
    10801 cacacgcacg acctgtcgcg aaacgccttc tcgcgcggtg agccggcgca tggcgtgcaa
    10861 ctcgctgatg acctcgagcg gatcgttgcg ttacatgacg cctcgacgat tgctgcggtc
    10921 atcgtcgagc cgatcgccgg atccacgggt gtgctgatcc caccgcgcgg gtatctgaag
    10981 cggctgcgcg agatctgcga ccgccacggc attttgttga ttctcgatga ggtgatcacc
    11041 ggctttgggc gtaccgggtc cgcctttgcc tcacaggaat tcggtgtgac gcccgatctc
    11101 gtgactttgg caaaaggact cacgaacggc tgcatcccga tgggtgccgt gctcgcgaag
    11161 aaggcgatct acgatgcttt catgcagggg ccggagggct cgatcgagct gttccacggc
    11221 tacacctact ctgcgcatcc ggccgcgtgt gcggcagccc tggccgcggt cggcatctac
    11281 cagcgtgagg gcctgctcac tcgcgccaga acactgtcgg atctgtgggc ggacgccgcc
    11341 cattcattgc ggggcctgcc gcacgtcatc gacgtgcgca attacggatt gattgcgggc
    11401 atcgagctcg acccgattcc gggaaagctc ggcagccggg cgcacgaggt ctttctgaga
    11461 tgcttcgagc gtggactgct catccgtcag acgggcgaga ctttagcgct ctccccgccc
    11521 ctgatcatcg agcgctcgca cattgaccag atcttcacga ccgtgaagga agttctgcgc
    11581 acgatgtgag ggtgcggcgc gggacgcttg ctgttcggtc ccggcccgat cgcctccgcc
    11641 cctgaaaaac agccgaggcg cggcgtgcgc atcggctgtg agagctatcg tcgtgctctc
    11701 tcggattgac gtcaggcgtg aggttcctgg tggcggctat gcaggtgctt caaccgggac
    11761 aacacgatcc acgccaaagc ggcgatggtg accgcgcttc ccacgagc
//
